LOCUS       DBMDUA010000001        53675 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_599_length_53675_cov_3.703177, whole genome shotgun sequence.
ACCESSION   DBMDUA010000001 DBMDUA010000000
VERSION     DBMDUA010000001.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 53675)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 53675)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53675
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_599_length_53675_cov_3.703177"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(128..457)
                     /locus_tag="ACZPCL_00005"
                     /pseudo
     CDS             complement(128..457)
                     /locus_tag="ACZPCL_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            complement(450..1070)
                     /locus_tag="ACZPCL_00010"
     CDS             complement(450..1070)
                     /locus_tag="ACZPCL_00010"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1160912.1"
                     /translation="MKGIICIGRQYGSGGREVGEKLARKLGIPCYDKLLIKKAALESG
                     LSTDFISKTEESPINSIQFLSGNPYADIASIGNTFYSESQVTFNAEKEVIEKIAKQGP
                     CVIIGRCAASMIPKENRLSVFIYSDKESTVKRVMKRNQISEKEAVHRIKHMNRMRKHF
                     YEFYSETEWGQSESYDMMLSSSKLGIDGCVDVIIDGLRGMGEMENE"
     gene            1362..2267
                     /locus_tag="ACZPCL_00015"
     CDS             1362..2267
                     /locus_tag="ACZPCL_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1160913.1"
                     /translation="MDISYDYYRTFYYVAKYKSFTGAAEALLNSQPNITRTMKKLEKE
                     LGCTLFVRQRHGIALTPEGEKLYAHISVAFEHISYGEQEIARDKSLQSGIVTIAASEI
                     ALHCILLPTLKKFRNTYPGIHIRILTHSTPQAMDMLKRGLADFAIVTEPFEFISGFSF
                     RILRSIQEVPVCSSVFSLSTGTSLTDEQIKKYPIIGLGEKTSSFAFYSDYFNRRGGTF
                     APEIEAATADQILPMVQSDLGIGFVPTDFLEKENMENLIILNMEPPIPKRNICMIKRK
                     YESLSIAAAELEKMILNNNSNNKRN"
     gene            complement(2347..3168)
                     /locus_tag="ACZPCL_00020"
     CDS             complement(2347..3168)
                     /locus_tag="ACZPCL_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1160914.1"
                     /translation="MKKQYLAVAFLAVALGITACSSKKADTESTTAQEVESTEATSDE
                     EVEEDYYYGFVSLVEDNVITVSEDGGNTAKFDISDAEITGADEIGEGDEVNVGFAGEL
                     SSDVTKATSVEIMTSAAEVAREEEADQEDLVVEGTIESADDSTITLKTEEGTYTLNAL
                     IAQKVTKDGIKAGAEAEVTYYGDLEDPDDAAVATRIVTADAKDTEEAKKKTLTGTVAE
                     VEADHIVLDTADPENTLFSFVGTEGMFDGISVGDKVTVIYDGTLTDKTVAAQGVE"
     gene            complement(3295..4248)
                     /locus_tag="ACZPCL_00025"
     CDS             complement(3295..4248)
                     /locus_tag="ACZPCL_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1160915.1"
                     /translation="MKEKNRTWGKWYLKGLAFLATGIFTSMAAGLSVMAQTELTFTEK
                     LTIEEVRELSEPEKDYEDENGIRYELKHWELQEKEGEEITRTLEKRVEYPKVEAAEEV
                     PEIILADETDPGWILLSGNLYRKNVEITGKQWKEDFSVPVTFYSYGADEYELGDLVIP
                     AGEDQLLEKTAEAGDRILEDLGLSKDDYRITSLVWNGEPFSDENGQICREALALGKRL
                     LRDYEVLYEGEVKWKEPDYYELHVIYQLEEPAKMTEEETTMEIKEDHKEKNDSPVKRQ
                     GPFWYLVRTGLMITVAIGILGLFIGLILLVVMKRRSRKDRS"
     gene            complement(4502..5785)
                     /locus_tag="ACZPCL_00030"
     CDS             complement(4502..5785)
                     /locus_tag="ACZPCL_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR1160916.1"
                     /translation="MRKMKSRVLAVLMAAGMIFTAQGMTVLAGTTGIGAAKTASQTGG
                     PGEVKQDIGTVTVDDTQETSGETGQNTDTPLQVNYSVYFRNQGWSDPAADNQALSASS
                     ESWVTSMKANLINIPSGVQIGVRYKVNLSGTGWLDWKADGVENGGASAEKPLEAIAME
                     LTGSSAASYDLYYKVYQNGSWTDWAVNGATAGTEGAGLRVDGIKASITAKDAGAPAET
                     ASSTVDPSKPMIALTFDDGPRASVTNRILDSLSQYGGRATFFMVGTNVPHNGDVIRRM
                     VAQGCEVANHTNDHKYISKLSSDGIVSQVSAVNQKVAAVCGVSPVVMRPPGGYVDAHS
                     LSVLGSMGMPAIMWSIDTRDWQHRNAQRTINNVLSQVKDGDIILMHDIYDATADAAVV
                     LIPELTARGYQLVTVSELAAARGGMSAGHKYSQFR"
     gene            complement(5798..6328)
                     /locus_tag="ACZPCL_00035"
     CDS             complement(5798..6328)
                     /locus_tag="ACZPCL_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1160917.1"
                     /translation="MIKEAAEFADRAHQGVFRKGTEIPYITHPMETAAIVTAFTDEPE
                     MIAAALLHDVIEDAGVTREELEEKFGPRVAFLVDGESEDKSKSWVERKGATVERLKTA
                     TRDEKILALADKLSNIRSTARDYLVLGDEVWQRFNQKDKEMQGWYYKGVAEALKEFKG
                     HIYYEEYVMLCERVFG"
     gene            6546..7736
                     /locus_tag="ACZPCL_00040"
     CDS             6546..7736
                     /locus_tag="ACZPCL_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1160918.1"
                     /translation="MEHHSSYSAPRSSRGSEHTRPAASRPSQRRSRSPKKGSSFGHIL
                     LCYILPFLAVNLAIFFIVTATPKIEMTISDTTDYKTLDVSFKVHSLLPLKKMTTTLES
                     QPIEFQKEKGVYHAVLTNNGTLEVYVLSLNGMQAQDIEHIAVLDDTPPSFDEESVVTE
                     NGTLTITIEDSISGINYDSLYATDDAGTKILPDSYDKENGEVTFKLEEGSLTVYVEDM
                     AGNPNQASFSLSQSGIDTEDRESKFPTDGKNGMNGTTATETENETKASETKSSETKAS
                     ETKSSSTSSTKAETKAETKASSAAKSTTAAAKSTTAAAKSTTAAKSTTAAAKSTTAAA
                     KSTTAAAKSTTAAAKSTTAAAKSTTAAAKSTTAATTEAAKSTTAAAHEPSTAEAPATI
                     APLN"
     gene            complement(7843..8850)
                     /locus_tag="ACZPCL_00045"
     CDS             complement(7843..8850)
                     /locus_tag="ACZPCL_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR1160919.1"
                     /translation="MIKKKIFCLWAAALILSGCAGVFQGKEKETEPDFVLTYAENQAE
                     DYPTTQGADKFAELVKEKTEGKVEILVNAGGSLGDEKSVIEQLQFGGIDFARVSISTV
                     AEYIPKLNVLQMPYLYTGADHMWKVLEGPIGDDFLNSFDGSGLIALSWYDAGARSFYN
                     SIKPIEKLEDIKGMTIRVQESELMSDTVRALGAVPVPMIYGEVYSGLQTGKVDGAENN
                     WPSYESAGHYEVAKYFTVDEHSRVPEVQIASTYTWNKLPEEYREIIRECAQESAWYER
                     SQWKARESISRQRVETKGCVITQLSPEEKLRFQQAVMPLYEKYCAEYVDIIDEIVAEG
                     W"
     gene            complement(8887..9372)
                     /locus_tag="ACZPCL_00050"
     CDS             complement(8887..9372)
                     /locus_tag="ACZPCL_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HIR1160920.1"
                     /translation="MYQYRYKTTRGEYWKMSMYFIYHSMTGMVNLVFTAALIALTCAK
                     WESSGTVFRICMVLGCCLFPILQPLAVYLSAGKLASGSGEDTEIRLDDVGIQVKVGNT
                     YDKIPWRKMVRIAKLPGMAIIFTDPSHGYLLTDRVIGEDKATFYDWLMKKQSQIQVKS
                     K"
     gene            complement(9603..10898)
                     /locus_tag="ACZPCL_00055"
     CDS             complement(9603..10898)
                     /locus_tag="ACZPCL_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR1160921.1"
                     /translation="MDVNSMAILILLGSFFLMIMLRFPIAYAVALSSVFCLMAQGLPL
                     TTICQQMVKGISSFSLMAVPFFITMGCLMGSGGISEKLIALANACVGWMRGGMAMVNI
                     VASYFFGGISGSASADTASLGSILIPMMVDQGYDGDFSTAVTITSSCEGLLVPPSHNM
                     VIYATTAGGISVGSLFLAGYVPGALLAASLMVGSYIISVKRNYPKGDKFSMKNLLKQL
                     SVSFWALAAVLIVVFGVVGGVFTATESAAVAVIYSLIVSVFIYKGLDWKGVWRVLGEC
                     VDTLSIVLILIATSSIFGYCLTRLHVPDLAAQAIVGLTDNPILIALLLNLILLVLGCI
                     MDMAPIILIATPILLPIATSIGIDPIQFGIMVVLNCGIGLLTPPVGAVLFIGSAVAKI
                     PMEKVVKATLPFYLCMIITLLLITFVPGISLWLPSVFAH"
     gene            complement(10900..11421)
                     /locus_tag="ACZPCL_00060"
     CDS             complement(10900..11421)
                     /locus_tag="ACZPCL_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR1160922.1"
                     /translation="MPSIFEKIDKIRPLYDAVYKFVLFICKLLLVVDIAITSMSVAGR
                     YISFIPDPAWSEEVVLSCMAYMAVLSAALAIRRGAHIRMTALDSYLPKKLVKSLDILA
                     DVAVLVLAVIMLTVGWTYASGIGSKGTYVSMPGVSRFWMYLPVPLAGVAMIIFEIEAL
                     YNHIRSFYVKEEK"
     gene            complement(11513..12631)
                     /locus_tag="ACZPCL_00065"
     CDS             complement(11513..12631)
                     /locus_tag="ACZPCL_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR1160923.1"
                     /translation="MKKRWLSAALCTAMVAGSLAGCGLKSPDTATTAAATEAAKEEAT
                     TAADAKEETKEEAKAEAGEPVGPAVTLVMAEVNPLDTIVGQTDSAFKEKVEELSGGSI
                     TVDLQASGVLGSENDVLDTMLGGGGTIDMSRISAFALTSYGAEKSVLLSVPYTFQNRE
                     HFWKFADSDLAPEFLMEPHDNGLGVRGLFYGEEGFRHFFTVKPVNGLEDLKGMKLRVS
                     NDPIMNGMVAGLGANATVVSFNELYSALQTGVVDGAEQPIANYQSNAFPEVAPNLILD
                     GHTLGAIQVIITDDAWDKLTPEQQDVLTEAGKYASEFNRKISEEAENKVLDELKANGV
                     NVVEVEDKTPWQEACKDIIESSTKDNAELYQQILDMAK"
     gene            complement(12788..13594)
                     /locus_tag="ACZPCL_00070"
     CDS             complement(12788..13594)
                     /locus_tag="ACZPCL_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1160924.1"
                     /translation="MYRLLIADDEMIERKVLFKTLSQNLKGQCEIFQAENGREALEIY
                     EKEKIQIAILDIEMPGINGIQAAERIREKDKKCCIIFLTAFDEFAYAKKAITVRALDY
                     LLKPWDEKELMLVLEEAMRMAGEEKDFQKEKTEEVSEEEKRDDLEELAEDDAGENLRM
                     NKVTEIIRDYIRENYMYDLSVQDLADKMNYSEPYFCRLFKQSFGQNFTAYLTEYRVSM
                     AKKMLEEPTVNIKDIGKSVGYEDSNYFTKVFRRITGQSPTEYRNSVFREK"
     gene            complement(13569..15095)
                     /locus_tag="ACZPCL_00075"
     CDS             complement(13569..15095)
                     /locus_tag="ACZPCL_00075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1160925.1"
                     /translation="MRPSLRKLWMTISIKRKIGIFAAMVILIMALSASFNVWITNVSL
                     GSFGGILNDNSRCNDFQEALDQEIRAFEHYVRNADQESRDTYRVACVRTERCLWSLPS
                     EYRRIGKERSARTWNIRNAYEGYKVYRDDVAEGNKTGSEFVDELYYVYKMQEYLQGYT
                     GRLVQITMAEGTEEYQKEAAMFSNIPYIIVAISIALMIIAIWLTRILSNSVAKPIVSL
                     AASTRKIAANDFDEPDLIIENKDEMGELVTAFNVMKHSTKGYIATMKENNKMQELLHK
                     EELERSEMEKQLGSARLELLKSQINPHFLFNTLNMIACTAKLEDAVDTEKMILSLGNL
                     FRYNLKTREQTVTLDRELKIVNDYMYLQKMRFGSRIKYRLSLETDAADVIIPSLSLQP
                     LVENAVVHGLSKKETGGILHIRIWKKDRMLILSVADTGLGMSQERCEEVAAAMKEHRT
                     AKIGIGLGNIYKRVHMMYQKGDLRIYSREGKGTVIQLMIPQGEETQGEEVYVPTADRR
                     "
     gene            complement(15150..17918)
                     /gene="glgB"
                     /locus_tag="ACZPCL_00080"
     CDS             complement(15150..17918)
                     /gene="glgB"
                     /locus_tag="ACZPCL_00080"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR1160926.1"
                     /translation="MEQKLYDIMDWAGIEELTYSESADPHRMLGPHATEEGLLIQAFI
                     PTATAITVKLTGNGKKYPMELADEAGFYAVLIPRKTVTDYTLLVTYDNGTEEEIHDPY
                     SFGPQFTEDELKKFGAGIYYDIYEKMGAHPMTIDGVEGVYFAVWAPCAMRVSVVGDFN
                     LWDGRRHQMRKVGEGDASVFELFIPGLKAGCLYKYEIKTRAGLPMMKADPYGNYAELR
                     PNNASIVWDINNYQWKDKKWMDKRAASDTKDKPFNIYEVHLGSWMRKEIAKDENGEDI
                     IGSEFYNYRELAVKLAEYVKDMGYTHVELLPIMEHPLDASWGYQVTGYYAPTSRYGTP
                     DDFMYFMDYMHEQGIGVILDWVPAHFPRDAFGMACFDGTCVYEHADPRKGAHPHWGTL
                     IYNYGRPGVSNFLIANALFWAEKYHADGIRMDAVASMLYLDYGKNDGEWVANIYGGNE
                     NLEAVEFLKHLNTVFKGRKNGAVLIAEESTAWPMITGDPKDGGIGFDYKWNMGWMNDF
                     TNYMRCDPYFRKNNYGELTFSMLYAYSENFVLVFSHDEVVHGKGSMMGKMPGETLEAK
                     AENLRAAYGFMMSHPGKKLLFMGQDYGQIDEWNENASLEWDLLKYPLHKNMQTYVREL
                     NKLYQAHPALYEQDFDTEGFEWINCSYHEESMIMFLRRGKEETLLCVCNFDNMDHEKF
                     RLGVPFAGKYKEIFNSDAKEFGGEGRTNSRVKASKKIEWDERENSIEIHIPPMSFMVF
                     SCTPEEKKESPKRLTTKKAEPKKLATKKEEPKKLTTEKKEEPKKLATKKTEPKKLTAE
                     KKEEPKKLATAKTEPHKLETKKEEPKKLATAKTEAHKLETKKEEPKKLATAKEEPKKL
                     ETAKPEPVPVLAPADPIAEPVTTPASAVTKPEPAPSPAPAAPAPTSAAAKKAALRAAK
                     KRGRNKKR"
     gene            complement(17957..18421)
                     /locus_tag="ACZPCL_00085"
     CDS             complement(17957..18421)
                     /locus_tag="ACZPCL_00085"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HIR1160927.1"
                     /translation="MEFVPMTIKKQKNIALIAHDQKKHELMEWCKEHKSILEKHFLCG
                     TGTTARMITDYTGLPVKGYNSGPLGGDQQIGAKIVEGQIDFVIFFSDPLTAQPHDPDV
                     KALLRIAQVYDIPIANNKASADFMISSPLMDEEYEHDVINFRQNISERAKQL"
     gene            complement(18527..19423)
                     /locus_tag="ACZPCL_00090"
     CDS             complement(18527..19423)
                     /locus_tag="ACZPCL_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR1160928.1"
                     /translation="MRLVTYEVEHKGGLGVISRDGKWVYPLRSLDMDYKTMQELIEGI
                     SESEKQLLEYVSGQDPYKIRGAAPIEEVKFLAPIPNPKQDVICLGINYMAHAEESARY
                     KKEAFGGERPYAVYFSKRVNEAVANGEGIESHSDIVADLDYEVELAVIIGKEAKNVPE
                     EEVKNYIFGYTIINDVSARTLQTQHNQWYFGKSLDGFFPMGPCITTVEDIPYPPKLSI
                     QSRVNGELRQDSNTELLIFGIDHIVSELSKGMTLKPGTIIATGTPAGAGMGFTPPKFL
                     KPGDVVECTIEGIGTLTNPVVE"
     gene            complement(19550..22189)
                     /locus_tag="ACZPCL_00095"
     CDS             complement(19550..22189)
                     /locus_tag="ACZPCL_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR1160929.1"
                     /translation="MAQWYGLTQEEVLKRLGTNRTGLNEKEAERRLETYGENVLKEQE
                     EMPWWQVFLGQFKDLLVMILIGAAGVSMVTGDPESALVIFSVLLLNAILGTVEHQKAR
                     KSLESLKRLASPETLLIREGRVCTAPSSRVVPGDILVLETGGMVPADGRILQSCSLTC
                     NESSLTGEALNVEKREGKLPAGKTPLSPGDQSNMVFSGALITGGRGTAVVTETGMDTQ
                     IGTIASLMNRTVEKKTPLQASLDRFSGHLALGIMGICGVIFLISLYRREPVLDALMFA
                     VALAVAAIPEALGSIVTIVQAMGTQKMAKEHAVIKDLKAVESLGCVSVICSDKTGTLT
                     QNSLRAEAVCINGKVMDTGQLKKYSHHPDLSLFLRSVILANNASGEETGEKEDPLEQA
                     LLHMAEEAGKDPGLLRRQWRRISEIPFNSDKKYMGAICSKGGEKILFVKGAVDVLLSK
                     CSLAVNPSFQEEETASWGRPFSFSDKERILRQNGLWSKKGMRVLALACKPLKEEEIRD
                     AKAENMIFLGLAAMTDPPRPESKMAVADAKKAGIRTVMITGDHKETAAAIASEIGIFT
                     AGDLAVTGADLDQMSDQQLQERLEKISVYARVSPEHKIRIVEAWQKKGHITAMTGDGV
                     NDAPALKKADIGIAMGKAGTEVSRDAAAMILTDDNFATIIKAVANGRNVYRNIKNSIQ
                     FLLSGNMAGILCVLYTSIKALPLPFAPVHLLFINLLTDSLPAIAIGMEPADRDLLNQP
                     PRDPKEGILSFPFAGKILFQGFLIGAATMWAWEMGYSQGGEAIASTMAFSTLTLARLF
                     HGFNCRGRKSLLSLGIGSNLWCVMALFVGVVLMCAVLFVPQLQDVFAAGDLTGSQLLA
                     VVLGALAPTAVIQIYKMMSAGTA"
     gene            complement(22324..23475)
                     /locus_tag="ACZPCL_00100"
     CDS             complement(22324..23475)
                     /locus_tag="ACZPCL_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007035584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1160930.1"
                     /translation="MLNEAMERFIKEHEKEAYDLLLTIAKIPSPSNHEEKRAQFCCNW
                     LKEQGAEGVYIDEALNVVYPAALRDNVDVYMAHTDVVFPDETELPLKVENGRICCPGV
                     GDDTACLVCVLLAAKYTALMIKEGKLNQIRPETEPGLIFVCNSGEEGLGNLKGVKKIC
                     EDYGSRMSTFCTFDGSLEHLVDEAVGSNRYKVTVRTQGGHSFGNFGNDNAIEKLAGII
                     EKLYQIKVPEGGKTTYNVGTISGGTSVNTIAQEASMLYEFRSDRKVNLAYMEEKFEAA
                     VNEAKAAGLDITYEILGIRPCSGDVDKERQARLLERAKAAVETVTGICPGVGSGSTDC
                     NIPLSMGIPSVCVGCYSGAGAHTREEYVEEASLPLGYRVAFEMIFGGRS"
     gene            complement(23465..25408)
                     /locus_tag="ACZPCL_00105"
     CDS             complement(23465..25408)
                     /locus_tag="ACZPCL_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HIR1160931.1"
                     /translation="MKREYSIETNERKIYPLGVTKTPGGFDVAFVSEKSPALLLFEKG
                     SRKRMARLAFPKNARMGNVHFMTVKGDFTGLEYAFEEDGKEISDPFGTCFTGREKWGD
                     EKAAGQALHTPFEAVKEAFDWEDDKRPEIPANECIVYKIHPRGFSKHVSFKGESWKRG
                     TFGAVIDKIPYMKELGITTVEMMPPAEFDEIMEDAPGKVNYWGYCCGHSFAPKAAYAG
                     AAPGQKKDPVKEFKTLVKALHKEGLELVVELYFDGTQSPSYVVDVLRFWAAQYHVDGI
                     HLVGYAPLETIVKDPYLSDLKLWAKNWDEVRTATETDRGNRTKEEKKETGRRLSAYET
                     GFMLDMRSFLKGDEGMLNQVALHVKDNPDAVASINYMANTNGFTLMDMVSYDHKHNED
                     NGEENHDGTDYNQSWNCGAEGPVRKKKIIGLRKRQLKNALAMLFLSQGTPLLMAGDEF
                     GRTQKGNNNAYCQDNEISWLNWNLLDSHKGLYEFTKNLIAFRKKHSLFHRSTEPTLMD
                     HKSVGLPDVSFHGEKAWCPDFERYSRQLGVFYSASYGENTDGSEEPYFYTAYNMHWEP
                     HAFALPNLPSGYIWHQVLDTAEDSMNGFLPSGKERHLDDQKEALVLARSIQVFAGLKC
                     PEKKKTQSKGPKANRKEGTEHVK"
     gene            complement(25509..27386)
                     /locus_tag="ACZPCL_00110"
     CDS             complement(25509..27386)
                     /locus_tag="ACZPCL_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1160932.1"
                     /translation="MNLLTIEHLTKSYTERLLFNDTAFSINEGEKVGLIGINGTGKST
                     LLRIAAGLEEPDGGTVVKGRSLYIRYLPQIPEFTPGDTILDSIVRDNKNEPHFSSVEE
                     LKASAKTMLNRLEIEDFDAKVETLSGGQRKRVALASVLLSTADLLILDEPTNHLDSQM
                     ADWLEGYLKAFKGALLMITHDRYFLDSVTNRIVELDKGKLYSYQGGYEKYLELKAERL
                     SMAEAAERKRQSILRTEIAWMQRGARARSTKQKAHIQRYEQLRDQEGPEYDRNVQLES
                     IGSRLGRTTVELKDICKSYGDKVLMKDFTYIFLKNDRVGIIGQNGSGKSTLMKIIAGW
                     VQPDSGTVEIGQTVKLGYFSQENEAMDESLKVIDYIRNAAEYVKTKDGSISASQMLER
                     FLFPSSMQYTTIGKLSGGEKRRLYLLRILMEAPNVLILDEPTNDLDIQTLILLEDYLD
                     TFPGIVITVSHDRYFLDRVVNRIFAFEGNGVVQQYEGGFTDYQRAWNERHPQISGNGA
                     GAGNAGSGKASDKDIDEENAANAKAVNKNNWKETSGAQKKLRLSYKEQREWETIEGDI
                     AGLEEAIAALETEIEKSASNYTRLNELMKEKTEKEAQLEEKMDRWMYLTDLVEQIESQ
                     K"
     gene            complement(27519..29078)
                     /locus_tag="ACZPCL_00115"
     CDS             complement(27519..29078)
                     /locus_tag="ACZPCL_00115"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR1160933.1"
                     /translation="MMNDNEIIRDFFMSRLERDGKSETLLDYTTGIRYYYQDFEVRSR
                     KLANFLTDKAKIKKGDRVAICAGNCVQHIDMFYAIVRIGYIMTSYNVKLTERELSELI
                     LKEKPSVLLYEEEFKEKAAALKKRFSELAFISIGDKKQEGDLACYQEVLEYSDEDRPL
                     PEHQFEDIAMLVHTGGTTGIPKAAMISYRALYYNVINQVTAYGVNNRDVVYVCLPLFH
                     AAAWNSLLMPLLYVGARMVMTRKFDKKDVFQIMKQEHLTFLLGVPTIFQYMMEDPAFE
                     TADFSEIGRIRCGAAPASLEIMRAYWKKGVRFCNGYGMTEVGPGILAMPINSMSVKDI
                     EEKRLSVGKPMVYVKLRIVNEKGEDVPKGEKGELFIRSAAMFSGYWGDPEETELALQN
                     GWMHSGDIAQQDEEGYYYIVGRKKNMFISHGENIYPVEIENIMKECPGIHEVCVIGVP
                     DKRKGEVGKAIVVLREGSKATAEDIKEYCSKNLAKIKQPKYVEIVEEIPRNSVGKVLM
                     SKVHELYGNVD"
     gene            complement(29091..30509)
                     /locus_tag="ACZPCL_00120"
     CDS             complement(29091..30509)
                     /locus_tag="ACZPCL_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012686.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR1160934.1"
                     /translation="MIELFMVVAYFIITTAAGKIMNGKGDNSIQHFFVAKKHLGIGLT
                     VALMFGEMVAGSSTVGNAASAFKMGLSSVWTNWGMVLGVLVFVFCTAKFYRRAGHFGV
                     MSVPEAFAFRFDKRVRLVVLVIIMFVYGIIFSQQPIAAAALLSSMLGVNKAVLAWIVG
                     ILFAYMALHGLKGIAGMNVIHSLVMFAGMFVVSFLAVGKAGGLAYMMANTPAHYFTVT
                     YPDKITVAAQVLGAAFSMILSSTVVNCCLGAESLKTAKSGIVWSGILVIPFALMPALI
                     GIAGFVTMPDAQAGNIIYTMAESVHPVFSGIVSMAVLAAILSTGPGLLLSLSATLTRD
                     FYMLVRPQASEKEQIRFSKGSIVVMALVATGFGLTTSSILTQILGAFQIRSVAGVVLI
                     IALVWKQVSNRAAFWSILIGGILAGVWHFSGNPFGIQPFWAAVPASVVILTVLTMLEK
                     EKISPDYLAYYNKIKDVSDSEI"
     gene            complement(30543..31547)
                     /locus_tag="ACZPCL_00125"
     CDS             complement(30543..31547)
                     /locus_tag="ACZPCL_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR1160935.1"
                     /translation="MSDARNVWVFIEVVRGKIKGVSLELLGQGRKMADDLGEKLVAII
                     PGNEIEDFAKMAIHYGADEAIVVDQKELKDYSTDGYTKAMCTLIKKYNPAVLLIGATN
                     NGRDLGPRVSSRMQTGLTADCTELGVDSETRLVKWTRPAFGGNLMATILCPDHRPQIG
                     TVRPGVFKKPEEDTGRKGEIIHETVEFGPDEIRTRIVEVITEAGGADVNLEEAEIIVS
                     GGRGVGGPEGFEVLKELADEIGAQIGASRAAVDSGWISSLHQVGQTGKSVGPKIYIAC
                     GISGAIQHVAGMSSSDVIIAINKDPDAPIFNIADYGIVGDLFEIIPELTKRIRSSKAE
                     "
     gene            complement(31562..32353)
                     /locus_tag="ACZPCL_00130"
     CDS             complement(31562..32353)
                     /locus_tag="ACZPCL_00130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR1160936.1"
                     /translation="MKILVCVKQVPDTTEIKIDPVTNTLIRNGVPSIVNPFDKNALEA
                     ALQLKDKYGAEVTVLSMGPGQAAEALRECYAMGADNMVLVSDRAFGGADTLATSYTLS
                     AAIRALGEFDLILCGKQAIDGDTAQVGPEIAEHLGIPQVTYAADIELNDTTLTVTKEQ
                     EDCYEKIEVKLPALLTAVKSLNEPRYPNIRRKMQANRMEVRTLTAADLPELDPARLGL
                     KGSPTKVKKTFVPPVHSTGVRVEGKDAGEQAGNLLKLLSEAKITF"
     gene            complement(32391..33533)
                     /locus_tag="ACZPCL_00135"
     CDS             complement(32391..33533)
                     /locus_tag="ACZPCL_00135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR1160937.1"
                     /translation="MEWQLMTAEQKDITGLVRKFLENELAPLVQECDGVSRFPMEVFK
                     KMGEIGLWGMDIPAEYGGPGFDLQTLSLLREEFGRVDCGFAVSFGAATVGFKQVLAGG
                     SEEQKRAMAERLLNGEISAMCITEPQSGSDVAGTKTKAVRVGDEYIINGTKTFITNGG
                     LANVYVVVASTNPELGHKGLSCFIVERNTPGVSVGKEENKMGIRLSNTTDVIFQDVHV
                     PAENIIGKEGDGFKIVMKALEKSRPICVAPLIGTAQKALEIAAQYTRERCPYGKPIGK
                     LQGIQFKIADMEIKLQTARAMMMYAAKVVDEGKPISCLGSISKTYVSEAVNEIVDEAL
                     QILGGYGYSKDYPIEKMLRDVRVYRIFEGTNEVQRQIIGKTIIGKC"
     gene            complement(33566..34363)
                     /locus_tag="ACZPCL_00140"
     CDS             complement(33566..34363)
                     /locus_tag="ACZPCL_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012596.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR1160938.1"
                     /translation="MEVPELKYADFELKDGIGMITMNNPEKLNALTMEGAQSLTKILN
                     ACSDSPEVRVIVIRGAQGNFCGGGDVKGMKYKVDNNCCETRPGLRCNNDVILSIVNNA
                     KPVIAWLEGAVAGGGLSIALACDFSYAQLASKFVFAFVNIGFIPDMGSSLMLTKNLGA
                     AKAKELLMLGHRFTGEQAADWGIITAAAAPEDLEEMVMKTAKKLAKGPALAYERMKRL
                     VNRQLYQGLEDAMENEGEYQYQLCKSEDHAEAIHAFFEKRKPDFKGR"
     gene            complement(34392..35597)
                     /locus_tag="ACZPCL_00145"
     CDS             complement(34392..35597)
                     /locus_tag="ACZPCL_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014564.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR1160939.1"
                     /translation="MKMAGMLDDVKVLDFTTNAAGPVASAEMSDYGAQVIKIESMNGG
                     PERTYVPILEGKSLTHAWVNRGKKSITLNLKDPRAIEIVKALAKDADVLIESNRPGVM
                     KRLGLDYETLKEINPSLIYCSLTAFGQTGPYSQKPGFDMMGQALSGMMSITGEKGGQP
                     LKHGIALADYFGGFNVFASVLTALHYQRRTGKGQYIDVSLLQGMIHLNSTIDRLNDGV
                     LCKPNGNHHSGLCPFGCFNTDKGESVIICAPSAKAWEAVAKAMNRPDYLTDPEYNTIG
                     ARTKNMVKVIAEIEAWLSSFPSLKEACVRMDECHMAYCLVNNDQQVVDDPQVKHMGYI
                     VQAPTPDDIKQPTYQTRGCNAFFSDVPGEIHKAATLGQHNHEVLGGLGYSDEEIDQMM
                     KEFKEEMKK"
     gene            complement(35638..37050)
                     /locus_tag="ACZPCL_00150"
     CDS             complement(35638..37050)
                     /locus_tag="ACZPCL_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013132.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR1160940.1"
                     /translation="MSKKQIIGGIIGIAACLGLSVMAPPANLVVAEGLDPQKSMIGMG
                     LLVCALIWMIFRVLPEYIILLMFCCLCAATKSLTFAQAFSPFSGTNYWLLVGALVLGA
                     AVAHSGLLKRLTLLVMKAFPGTYLGQFLGITLSGVVISPLIPSGTARLAVAGPVTKEI
                     GHEMGLENQTKPMAGLFAALYTGFNCTSHVFLSASFMCYSMLKFLPEGYSDVTWTDWF
                     IWALPWSIVALGLTTIATYFYYKPTEKINLNKNFIANKIVELGTWNKGEKITMAVLAI
                     CLVLWMTERQHGISSAIVAMLGVCALMGLKVMGVKEFRAKVNWDILIYIGCMYEIGSA
                     LTTWGITKWIGGVLGPVLNPFLSNPVLFVLIFILLVYLTRFIVISWLGVMTVYSVLLA
                     PLAVQAGMHPFIPCFIAYVSVNVFFFDFQNLPYVSAIGIVGDMVDHNKNCVPYAIIWA
                     AANLVACLISVPYWKMIGML"
     gene            complement(37246..38205)
                     /locus_tag="ACZPCL_00155"
     CDS             complement(37246..38205)
                     /locus_tag="ACZPCL_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1160941.1"
                     /translation="MELLQLRYFCEVARRESVTKVAEELHVSQPSLSKTIKNLEQELG
                     VVLFDRVKKRIVLNEQGKEFYDKVSKGLNLIDSAVDGLSVTPDSENGTISLIVKAGQC
                     FFPPMYDLFCKLHPNIILDVANFSLMQRKLMTEYDFHISASMNTYENVEFEYLMREEI
                     VIVLNRSHPLAKKKLLHMSELREEPFIAGWRGSSSNTLMVSLCHAAGFVPKFCAYYSE
                     MERIFRAISENEGISIIPYDSLKDTLKEHEDLTYAFIADPGNYRLLKLCWPKKNFESR
                     RNQMFREYALNYFADLEKRLMEEKQCVFHNNCITHKEYILEYF"
     gene            complement(38260..39231)
                     /locus_tag="ACZPCL_00160"
     CDS             complement(38260..39231)
                     /locus_tag="ACZPCL_00160"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR1160942.1"
                     /translation="MFKTRFTELIGIEHPIMQGGMQHLGVPQLAAAVSNAGGLGTINI
                     TIYPEMDDFRAAVKKTKELTDKPFAVNISLAPGLNMGEKIHQYIDVCGEEGVKVIETA
                     GQNPSEFVPHIKEAGIKLIHKCPGVKYAKKAESFGADAVTIVGYEVAGHPGLDELGSF
                     ILINRVAESVSIPVLAAGGVADGKGLLAALALGAEGVVMGTRFVASTECTIHQNFKDL
                     IVNSKEIDTMTCQRSIKNMARYVKNEQSAKALEMEKAGAGLKELLPVISGALSKECYV
                     SGDTQHCVFAMGPAAGLINEVKPVKAIIDDMINEAEAVYKRLGTLQA"
     gene            complement(39458..40216)
                     /gene="xth"
                     /locus_tag="ACZPCL_00165"
     CDS             complement(39458..40216)
                     /gene="xth"
                     /locus_tag="ACZPCL_00165"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR1160943.1"
                     /translation="MKKLISWNVNGLRACVGKGFMDFFNEVDADVFCIQESKLQEGQI
                     DLDLPGYHQYWNYAEKKGYSGTAMFTKEEPISVSYGLGIEEHDHEGRVITAEFPEYYI
                     VTCYTPNSKDGLARLDYRMTWEDAFLAYLKKLEEKKPVIFCGDLNVAHKEIDLKNPKT
                     NRKNAGFTDEERGKFTDLLNAGFIDTWRYFYPDTEGIYSWWSYRFKAREKNAGWRIDY
                     FCVSESLKDELESAKIHTEVFGSDHCPVELVIKK"
     gene            complement(40355..40840)
                     /locus_tag="ACZPCL_00170"
     CDS             complement(40355..40840)
                     /locus_tag="ACZPCL_00170"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HIR1160944.1"
                     /translation="MEKIASFTIDHIKLQPGVYVSRKDKVGDSTVTTFDLRMTSPNEE
                     PVMNTAEMHTIEHLGATFLRNHKDFGDKTVYFGPMGCRTGFYLLLAGDYESKDILPLV
                     VEMYEFVRDFEGEVPGASPKDCGNYLDMNLGMAKYLAAKYLKVLTHIDEFPESRLVYP
                     E"
     gene            complement(41044..42681)
                     /locus_tag="ACZPCL_00175"
     CDS             complement(41044..42681)
                     /locus_tag="ACZPCL_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009219319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1160945.1"
                     /translation="MISANNISLRVGKKALFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGQLEPTSGEVAITPGQRLSFLQQDHFKYDEYTVLDTVIMGNARLYQIMKEKEAI
                     YMKEDFTDEDGIKAAELEGEFATMNGWEAESDAENLLNGLGIGTEFHYAQMSTLLGAQ
                     KVKVLLAQALFGNPDILLLDEPTNHLDLDAIAWLEEFLINFENTVIVVSHDRYFLNKV
                     CTQIADIDYGKIQLYAGNYDFWVESSQLIIKQMKEANRKKEEKIKELQEFIQRFSANA
                     SKSKQATSRKRALEKIQLDDIKPSSRKYPYIDFRPAREIGNEVLTVEHLSKTIDGEKI
                     LDDISFILGREDKVALVGPNERAKTVLFKILAGEMEPDEGSYKWGLTTTQSYFPKDNS
                     AEFANDDTIVEWLTQYSPEKDVTYVRGFLGRMLFAGEDGVKKVKVLSGGEKVRCMLSK
                     LMISGANVLMLDEPTDHLDMESIDALNRGMIKFPGVMIFSSRDHQIVQTTANRIMEII
                     NGKLIDKITTYDEYLESDEMARKRFTFTVTESDEEDD"
     gene            complement(42877..43557)
                     /locus_tag="ACZPCL_00180"
     CDS             complement(42877..43557)
                     /locus_tag="ACZPCL_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR1160946.1"
                     /translation="MKMVAEMITKIGIPSDAELFLRVIVASVLGYMIGYERKNRDKSA
                     GMRTHAIVALGAALMMVVSKYGFYDVEKVDSSRVAAQIVSGVGFLGAGVIFVRNNTVS
                     GLTTAAGLWTTAGVGMAVGAGKYGIGVCAGILVVVIQELLHRTSFLTAEPIRGFLKMT
                     CTDYENVIADIQMHFSEEKVKISNLKVGKGKNGTKIELEVVYPPLYDKNAMLLKLAAD
                     ERITGISG"
     gene            complement(43680..45059)
                     /locus_tag="ACZPCL_00185"
     CDS             complement(43680..45059)
                     /locus_tag="ACZPCL_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1160947.1"
                     /translation="MKILVIDDEPLIHISIEKLIQACSKEDEVLHAYNGHEMLKMLEE
                     HEIAMAYVDIKMPGPSGLEAIKLGKEISPDTRYYIMTGFNEFEYAKQAVKLKVEDYLM
                     KPLDKKTIQETITAVRLQQYLAGERRKNLFRTWLESALNGRNIGLGEYKGYFCGLLMI
                     TVDGENLVSDPIPALFQGYEDHVVSVFSDNSLKLLCFSEKGEGIQEIYRTLSARTFAE
                     GMTCFGTSVTRDMENLREDLGNLEELSCLRVIKGTGRFYYLSPLLDEEEEKVEFGRRC
                     LKWQEAWKSGDYNSFSSLSSWIGKKMEEKEWIGRSRKNIASFFSLVLKTPVSENMDPK
                     ELKNFLEQKSRSLLSSPSEDSRINSMIRFIEEHYREDISAADLSERFGLSASYISSLL
                     KTALGVRYSDYVAGLRLNHAKELLATTHMTVKDITVECGWYSQSYFTKLFIEREGCTP
                     VEYRKSFGK"
     gene            complement(45034..46764)
                     /locus_tag="ACZPCL_00190"
     CDS             complement(45034..46764)
                     /locus_tag="ACZPCL_00190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1160948.1"
                     /translation="MKQKKVQSLSRKLFVNFILSCLLPIVVVSFLVSYLFGTYQYQEI
                     RSRAENNTKLISAYMTKYLNDIDNLTKAPYYHSFFQSRTPFEDLTPYDQNKVGEEIGK
                     LLQLTTYSREDFGDMLVMSDGNVLYFNTSDWYQYLPTIHPLTGRSWYQSALDKNGRVA
                     IIPEKDMTGADEEEELDTGSFYISRKLNNMFLQDQENVIMLNLKTDDFENLFSSLTSK
                     APLIIAFTNDEDQLIYSNKPLTKTQTAGLKQQKVNYDHRNWLCFSQTLEHYPLTVHVL
                     LSASYVIDRMAIFLFMAAACCLLCSILGYAMFKKNNYWIERPTYHIKAVLGELQVGNL
                     NSRCEPLVIREFNEIGTSINAMAKQLQETIRNEYELKLAQKNLQLQALQSQIQPHFII
                     NTIYSFITLNQIGEQEALNDAFYSFAHLLRYVLSRSRETTLGAELDFLNDYCSLCLLR
                     FGNRLSYEINCSEEFKSLSMPKLLLQPLVENAVIHGIEPSEVPCKLFVEAERHGNSLY
                     ILIEDDGVGFKEEELNSPESIGIKNVENRISLWGPDIRLYIYRIDGRTIQVLIVPFKE
                     EKHEDTGDRR"
     gene            complement(46976..48310)
                     /locus_tag="ACZPCL_00195"
     CDS             complement(46976..48310)
                     /locus_tag="ACZPCL_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1160949.1"
                     /translation="MKRKVAGVLAAAMAAGMLAGCGSSAKPADTTAAKAEDTKAAEGS
                     TEAAAADNGDKITIEFWHCMGSSNGELIQQITDSFNESQDKIFVKAVHQGSYTDAGTK
                     MQAALAAGEAPVLAQMEIGMLGIFADADQLVDLQKFVDEENYDMSDFMPGLLDASYYD
                     GALVALPHSRSVPVLYYNKDRFKEAGLEEAPVTWDDLKADAKALTGNGSYGYSCPLDQ
                     WYYMSLVMNAGGTIFNETEDGIGFAGDPGTKPLYLWKEMIADGTMHVPSGQDYNSSEA
                     CRNAFAGGTAAMIQQSSAQLKGLEKTCEFEVGVGAVPQDTTLSYPAGGSNLLMFKGHS
                     EEEEKAGWEFLKYMTNTENAVRWANGTGYLPTRQSCIDSEEYKKMVEEDPNLQIIVDQ
                     VQYSTYKTPFIPQYQEAKEIFANEIQKCILEDNYTPEQAVSSMEERVAKLFK"
     gene            complement(48355..49200)
                     /locus_tag="ACZPCL_00200"
     CDS             complement(48355..49200)
                     /locus_tag="ACZPCL_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1160950.1"
                     /translation="MEESRGALLFQKVCHILNIILKVAVMVIIAFPFYWMVSTSLKDY
                     TEALQFPPTLFPSSLHWENYAHVFEIVPIGLYFRNSVVVSLCVLVLQYLIIVPAAYSF
                     ARHEYFGKNIFFGIVLLGLMIPQQITFLPVYLMFSRMKMLKTFIPLILPFISNPFGIF
                     MLRQYFMQIPQEVIEAARLDDADNRKIMFKVMMPMAKPALITIGLLSFISTWNNYFWP
                     LIMTSSETLRTLPVGIAALKSSETLQMWHIIMAGNVVLVLPILIVYMLASQKIRNSFV
                     YSGIK"
     gene            complement(49240..50142)
                     /locus_tag="ACZPCL_00205"
     CDS             complement(49240..50142)
                     /locus_tag="ACZPCL_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1160951.1"
                     /translation="MDKKKRSVSEKRDRMLGFVTAMPAMILLFVFTVYPVFYLIYYSM
                     FSGSLISAKKTFVGLDNYKELFGSETFRLVLVNTVVYTVLFVSLTMVLAVIIAVWLNG
                     SKHKKLNNMVEGFIFTPHVISMVSVSIVFLWLMDPDTGFLNMVITKLGFAKFPFLSSP
                     STSMFSLVLVMVWKSVGYYVLLIMAALQTVPSNIYEAAELDSTPKWRVFFKITLPMIS
                     PTILFTSVVASIASFKIFDSVNVMTQGGPVDSTNTLVYYIYSFAFRYGKPGMACAAGV
                     ILLILVCIMTYVQFGVGKKRVHYQ"
     gene            complement(50146..51201)
                     /locus_tag="ACZPCL_00210"
     CDS             complement(50146..51201)
                     /locus_tag="ACZPCL_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1160952.1"
                     /translation="MGAIQLKNVTKKFGDQMVIDDLNLSIEDGSFTVLVGPSGCGKST
                     TLRMITGLDEPTSGDIYIDGKKINDLTPGKRDIAMVFQNYALYPTMTVRENIEFGLEN
                     KKVPKEERKKRVQEICEVVGLTQYLDRKPATLSGGQRQRVALARAMVKQPKVFLMDEP
                     LSNLDAKLRGQMRVELIGLHKKLGTTFVYVTHDQVEAMSMADDIVLMKDGYIVQQSSP
                     RELYNNPNCVYAAQFIGTPQMNIVKDILPEGMQVGFRPEKVYLKEVEEEHVEISAKIA
                     TKEMLGSEIIYSLDSPAGKLMAKSDYEAEDESTLKLSIPVKNMYLFDKDGKRITLDDE
                     RRLMIHAGFAKLAGREV"
     gene            51505..52149
                     /locus_tag="ACZPCL_00215"
     CDS             51505..52149
                     /locus_tag="ACZPCL_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1160953.1"
                     /translation="MKKRHVYDLVILLAAGTIMMSACGKKPDAPADTTAPTQTESQTE
                     ASAEETTKPDETPSETQVPEDTESTKFYMLQGTVTKAEKDGSAFTLLADDGNEYTIAL
                     SDIRDVETEIEKNIQIAIACMKDASGDPEKAAYIVAFPEQEEWTISTDEGITTSNAMS
                     TFMIQTSDGKEIGFIKDNCPSDENALTSDSGDSVIVTYVTSDNANFPLEIRKGK"
     gene            complement(52320..53084)
                     /locus_tag="ACZPCL_00220"
     CDS             complement(52320..53084)
                     /locus_tag="ACZPCL_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1160954.1"
                     /translation="MKLLEINHVEKSFDDLNVLKDISLSVEDGEIVSIIGPSGSGKST
                     LLRCATMLETMDSGEIVYLGKKAVWSDENGKLNYADKKDLKEIQSQYGLVFQNFNLFP
                     HFSVLKNITDAPIHVQKRDKEEVYKEARELLKKMGLEDKENAYPCQLSGGQCQRVAIA
                     RALALKPKILFFDEPTSALDPELTGEVLKVIKSLADLHIAMVIVTHEMAFARDISHRV
                     IFMAGGVIVEEGAPEKVFASDNERTRSFLGRYGNLL"
     gene            complement(53115..53603)
                     /locus_tag="ACZPCL_00225"
                     /pseudo
     CDS             complement(53115..53603)
                     /locus_tag="ACZPCL_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568971.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
BASE COUNT        13764 a        13316 c        10417 g        16178 t
ORIGIN      
        1 cagatatggg cagaatccgg aaaatcggct ctgcccattg taactattca caaatcttat
       61 atcagttttt aacgtttaca caaaacttga aacggtctcg ttttaacaat gcttcggatc
      121 ccaggatacc gatcagcatc aggcacacgc atacaaaggc gctgaaaatg caggctgtat
      181 aaacagctgt tttcattccc ttaaaatctt ttgcgccaaa cagctttgat acaactacag
      241 agcatcccat attgcagcca aatgcaaagg caatgaagat caatgtaatc tcataactgt
      301 ttcctactgc cgccagggca ttttcaccga taaattttcc tgctaccagg ctgtctgcga
      361 tgttgtataa ctgctggaag ataatacttc caaacaacgg cagacaaaat tgccagagca
      421 ccttttcagg atttcctact gtcagatcct tattcatttt ccatttcccc cattcccctt
      481 aagccatcaa tgatcacatc cacgcagcca tcaattccaa gtttactgct ggataacatc
      541 atgtcatagc tttctgactg tccccattca gtctcggaat aaaactcata aaaatgtttt
      601 ctcattctgt tcatatgttt gatcctgtgg accgcttctt tttcactgat ctggtttcgc
      661 ttcataacac gcttgactgt actctcttta tctgaataaa taaatacaga cagtctgttc
      721 tccttcggga tcatggaagc agcacagcga ccaatgatca cacatggacc ctgttttgcg
      781 atcttttcga tcacttcttt ctctgcattg aatgtcacct ggctctctga atagaaagta
      841 ttgccaatgc ttgcaatatc cgcataagga tttccactta aaaactgaat actgttgatc
      901 ggtgactctt ctgttttgga aataaaatct gtgcttaacc cggactccag cgccgctttc
      961 ttgatcagca gcttatcgta acagggaatt cccagttttc ttgccagctt ttcaccgacc
     1021 tcacggccac cacttccata ttgtcttcca atacaaataa tgcccttcat gtgatccact
     1081 tctttcttat ttattttaat taatgttgtt gaatgaatat tgaatgttgt tttttgacgt
     1141 cggctgtatt ataatccgta acaagatata tgtaaaatat taaaaatgta atttagatat
     1201 gtgtttttga tataatgagc gccatgaaaa acaagcgccg ccaaaggcgg catttgtttt
     1261 tcacaagcca ttaacgaagg ttccgcctgc gaatgcaggc agtaaagcgc gtcagcgcgg
     1321 ggaacctgag tatacaagtg tatatatggg ggaaaaccaa aatggacatc agctacgatt
     1381 attaccgtac cttttactat gttgcaaaat acaaaagctt tacaggggct gcggaagcac
     1441 tgctgaacag tcagccaaac attacccgga ccatgaaaaa actggaaaaa gagctgggat
     1501 gtactttatt tgtacgccag agacacggta ttgcacttac tccggagggc gaaaagctgt
     1561 atgcccatat ttccgttgct tttgaacata tttcctatgg ggaacaagag attgccaggg
     1621 ataaaagcct gcagtccggt atcgttacca ttgcagccag cgagatcgcc ctccactgta
     1681 tccttcttcc caccctgaaa aaattccgca acacctatcc aggcatccac atccgtatcc
     1741 tgacccattc cactccccag gccatggata tgttaaaaag gggactggca gattttgcca
     1801 ttgtaacaga accttttgaa tttatatccg gattttcttt ccgtattctc agaagtatcc
     1861 aggaagtgcc tgtgtgcagc agtgtttttt ccctttctac cggaacgtcc cttacagacg
     1921 agcagatcaa aaaatatccc atcatcggtc ttggtgaaaa gacaagttcc tttgcctttt
     1981 actccgacta ttttaacaga aggggcggta cctttgcccc ggagatcgaa gcagcgactg
     2041 ctgaccagat cctgcctatg gtacagtctg accttgggat cggttttgtt ccaacggatt
     2101 ttctggaaaa agaaaatatg gaaaatctga tcattttaaa tatggaaccg ccgatcccca
     2161 aacgaaatat ctgtatgata aaaagaaagt atgagtccct cagcattgct gctgcagaac
     2221 tggagaaaat gatcctgaat aataatagta ataataagag aaattaaaaa cagtaccccg
     2281 gaaccatcct gatctagatg atcccgggat actgttttta taagttgttc tttttatttt
     2341 tgtagattat tctacgccct gtgctgcaac cgtcttatcg gtcagtgtac catcatagat
     2401 cactgttacc ttgtcaccaa cgctgatacc gtcaaacatt ccttctgttc ctacaaaaga
     2461 aaacagggta ttttccggat ctgcagtatc cagaacaata tgatctgcct caacttctgc
     2521 tactgtaccg gttaaagttt tcttctttgc ttcttctgta tcttttgcat cagctgttac
     2581 aatgcgggtc gctactgctg cgtcatctgg atcttccaga tcaccgtagt aagttacttc
     2641 tgcttctgcg ccagctttga taccgtcttt tgttaccttc tgggcgatca gtgcattcag
     2701 tgtataggtt ccttcttctg tctttaaggt aatggtgctg tcatctgcac tttcaatagt
     2761 tccttctact accagatctt cctgatctgc ctcttcttcc cttgcaacct cagctgcgga
     2821 agtcatgatc tctactgaag ttgcctttgt aacatcactg cttaattcac cggcaaaacc
     2881 tacgtttact tcatcgcctt cgccgatctc atctgcacct gttatctctg catctgaaat
     2941 atcaaatttt gcggtattac ctccatcttc agaaacagtg atcacattat cttctaccag
     3001 gctgacaaag ccatagtaat aatcttcttc tacttcttca tcagaagtcg cttctgtact
     3061 ttctacttcc tgtgctgtgg tagattctgt atctgctttt ttagaagaac aagcggtaat
     3121 tcccagtgca actgccagaa acgcaactgc taaatactgt tttttcataa aaatctctcc
     3181 tctctctgcc gcctgtggac taactttttg cggcacgata catcttacta cgaaagctgt
     3241 gtttttacta tgtatttttt atgaattttt ccttaaatgt gtttttgggg atttttaaga
     3301 tctgtctttt ctggatctcc ttttcatgac cactaaaagg atcagtccta tgaaaagccc
     3361 aagtataccg atcgccactg tgatcataag gccggtgcgg accagatacc agaagggtcc
     3421 ctgtcttttg accggactgt cattcttttc cttatgatct tcttttatct ccatggtcgt
     3481 ttcttcctct gtcatttttg caggttcttc cagctgatag atcacatgaa gttcataata
     3541 atccggctct ttccatttta cttccccttc atacagcact tcataatccc gtaaaagccg
     3601 ttttccaagt gccagcgcct ccctgcagat ctgcccgttt tcatctgaaa atggttctcc
     3661 gttccatact aaagaggtga tccggtaatc atctttagaa agtcccaggt cttctaagat
     3721 ccggtcacct gcctctgctg ttttttcaag aagctggtct tcccctgccg gtatcacaag
     3781 atctcccaat tcatattcat ctgccccata ggaataaaat gtaacaggta ctgaaaaatc
     3841 ttccttccac tgcttccctg tgatctctac attttttctg tataagtttc cggaaagcag
     3901 gatccatccg ggatctgttt catctgccag aatgatttca ggaacctcct ctgcagcttc
     3961 caccttggga tattccaccc gtttttccag ggtacgggta atctcttccc cttctttttc
     4021 ctgtaattcc cagtgtttta attcatagcg gatcccattt tcatcttcat aatctttttc
     4081 aggttctgac agttctctga cttcttcaat tgtaagtttc tctgtaaaag tcagctctgt
     4141 ctgggccatg acgctaagac ctgcggccat actggtaaaa attcctgtag caagaaatgc
     4201 cagccccttc aaataccatt ttccccatgt tctgtttttc tctttcatac cttctccttc
     4261 acaatattta cgtaagaccc tgtgttttgg tttctgtatt taattgtcaa ggttcggttt
     4321 tcaatggatt tttagtgatt tcatgaagcc tgttatagct gtgatatggg agatctgaat
     4381 gtctcctgaa tgtttcatgg agctgttaaa aacagctgta ggtttattat aaaaaaggga
     4441 ggggattttg tcaaatacat ctggcaaata tcccctctcc ttgatggatc atttttaatt
     4501 attaacggaa ctggctgtat ttatgtcctg ctgacattcc gcctctggct gctgccagtt
     4561 cgctgactgt aactaactga tatcctctgg ctgtcagttc cgggatcagt actacggctg
     4621 cgtctgcagt agcatcgtaa atatcatgca ttaaaataat atcgccatct tttacctggc
     4681 ttaaaacgtt attgatggtc ctctgtgcat ttctgtgctg ccagtctctt gtatcaatgg
     4741 accacatgat cgccggcatt cccatagaac caagaacaga aagggaatgg gcatctacat
     4801 aaccgcctgg cggacgcatt acaaccggtg atacgccgca gactgctgcg actttctggt
     4861 ttacagcact tacctggctt acaatgccgt cactgctcag tttggatatg tatttatggt
     4921 cattggtgtg atttgcaacc tcacagccct gggctaccat acggcggatc acatccccgt
     4981 tgtgtggaac attggttcct accataaaga atgtagctct tcctccatac tggcttaaac
     5041 tgtctaagat gcggtttgta acagaggctc ttggaccatc atcaaaagtc agggcgatca
     5101 tcggctttga cggatctact gttgaagaag ctgtttctgc aggcgctcct gcatcttttg
     5161 ctgtaataga tgccttgatg ccatcaactc tcagtcctgc gccctctgtt cccgctgttg
     5221 caccgtttac cgcccagtct gtccaggaac cgttctggta taccttatag tacagatcat
     5281 aggaagctgc cgaagaaccg gtcagttcca tggcaatggc ttccaacggc ttttctgctg
     5341 atgccccgcc gttttctacg ccgtctgctt tccagtcaag ccagccggta ccgcttaaat
     5401 ttactttgta acggacgccg atctgtactc ctgatggaat attgatcaga ttagctttca
     5461 tagatgtgac ccatgactct gatgaggctg acagtgcctg gttatctgct gccggatcgc
     5521 tccagccctg atttctgaaa tatacactgt agttgacctg aagtggggta tctgtattct
     5581 gtcctgtttc tccggaagtc tcctgtgtat catctacggt tacagtacct atatcctgct
     5641 ttacctctcc cggtcccccg gtctgggacg cagtctttgc cgcaccaata ccggtagttc
     5701 ctgccagtac tgtcattccc tgggcagtaa agatcatacc tgctgccata aggaccgcaa
     5761 gaaccctgct tttcattttt ctcattctct tctcctttta accgaatact ctttcacaca
     5821 gcatcacgta ttcttcataa taaatgtggc ctttaaattc tttcagagcc tctgccacgc
     5881 ctttgtaata ccagccttgc atctctttgt ctttctggtt gaaacgctgc cacacttcat
     5941 ctcccagaac aagataatct ctggctgtac tgcggatatt gcttaatttg tccgcaagag
     6001 ccaggatctt ttcatctctg gttgccgttt tcaagcgctc tacggttgca cctttacgtt
     6061 caacccagct ttttgattta tcttcgcttt ccccgtctac cagaaatgcc accctggggc
     6121 cgaacttttc ttccagttct tctcttgtaa ccccggcgtc ctctattaca tcgtgaagaa
     6181 gggccgcagc tatcatctcc ggttcatctg taaatgccgt tacgatggcc gcagtttcca
     6241 tcggatgcgt aatataaggg atctccgttc cctttctgaa aacgccctga tgagcccgat
     6301 ctgcaaattc agccgcctct tttatcatat tgtgctctac ctcttttccc ccatgttcag
     6361 ttggcctctc tgataccaga ttgctacgtg cttttgaccc tggtatcatc ataccatacc
     6421 cggacattat tttgaagttg tattttattg tcactttcgt aagactttct caatgaataa
     6481 ttccttgtaa gtaaactgtt tttctgctat gctatagaga gtttttaaca aaggaggaaa
     6541 tggtaatgga acaccattca tcatacagcg caccaagaag cagccgtggt tcagaacata
     6601 cccgccctgc tgcttcccgt ccaagtcaga gacgcagccg cagtccaaag aaaggcagca
     6661 gtttcggaca tattttacta tgttacatac ttccattttt agcagtcaat ctggcgatct
     6721 tttttattgt cacggctaca ccgaaaattg aaatgacaat cagcgatact accgattata
     6781 aaacgctgga tgtaagcttt aaggtccact ctcttcttcc tttaaagaag atgacaacga
     6841 ctctggagtc ccagccaatt gaattccaga aagaaaaagg cgtttatcat gcagtcctga
     6901 caaacaatgg tactctggaa gtctacgtat taagtttgaa cggtatgcag gcccaggata
     6961 tcgaacatat tgctgtactg gacgatacac cgccttcctt tgatgaagaa agtgttgtaa
     7021 cggaaaacgg tactcttacc ataacaatag aagacagtat ctccggcatt aattatgact
     7081 ccctctatgc taccgatgat gcaggaacca agatccttcc tgacagctac gataaggaaa
     7141 acggggaagt cacctttaaa ctggaagaag gttcccttac tgtctatgta gaagatatgg
     7201 caggaaaccc aaatcaggct tccttctctc tcagccagtc cggaattgat accgaagacc
     7261 gggaaagcaa gttcccgaca gacggaaaga atggtatgaa cggaactaca gcaacagaaa
     7321 ctgagaacga aaccaaagcc tctgaaacta agtcttccga gacaaaggct tctgagacaa
     7381 aatcttccag caccagcagt accaaagcag aaacaaaggc tgagacgaaa gcatcatctg
     7441 cagctaagtc tactactgca gctgctaaat ccactactgc ggctgccaaa tctactaccg
     7501 cagccaagtc caccactgca gctgctaagt ctactactgc ggctgctaag tccactaccg
     7561 cagcagcgaa gtccaccact gcagctgcta agtccactac cgcagcagcg aaatctacca
     7621 ctgcggctgc caagtccact actgcagcaa ccacagaagc agcgaaatct actactgcag
     7681 cagcacatga accatctaca gcggaagcac ctgctaccat tgctccgtta aattaacaaa
     7741 taataaacaa caaaaagctg accgatcttc ttatttttca aagaatttct gtcagctttt
     7801 atttttatga tactattcag tcatggccga actgcaagta ttttaccatc cttccgctac
     7861 gatctcatca ataatatcca cgtattctgc acagtatttt tcatataacg gcataactgc
     7921 ctgctggaac cgcagttttt cttccggtga aagctgggtg atcacacagc cttttgtttc
     7981 tacgcgctgt ctggatatag actctctcgc tttccactgg cttctttcat accatgctga
     8041 ctcctgggca cattcccgga tgatctccct gtattcttcc ggaagtttgt tccaggtata
     8101 agtagaagcg atctgaactt ccggcacacg gctgtgttca tctactgtaa aatattttgc
     8161 cacttcgtaa tgccctgcag actcatagga tggccagtta ttctctgctc cgtctacttt
     8221 tccagtctga agtccggaat atacttcccc atagatcata ggaaccggaa cagctcctaa
     8281 agcccggact gtatcactca tcagttccga ctcctgaaca cggatcgtca ttccttttat
     8341 atcttccagt ttttcaatgg gttttatgga attataaaaa cttctggcac ctgcatcata
     8401 ccatgaaagc gcaatcagac cggaaccatc aaaagaatta aggaaatcat ctccaatagg
     8461 accttccagt actttccaca tgtgatctgc gcctgtatac aaatagggca tctgaagtac
     8521 atttaacttg ggaatgtact ctgctaccgt agaaatggat actctggcaa aatcaatacc
     8581 accaaactgc aactgttcaa tgacagattt ttcatctccc aggcttccgc ctgcattgac
     8641 cagtatctca actttgcctt cggttttttc ttttaccagc tccgcaaact tatctgcacc
     8701 ctgggtcgtc ggataatctt ccgcctggtt ttccgcatag gtcagtacaa aatccggttc
     8761 cgtttccttt tcttttcctt gaaatacccc ggcacagccg cttaaaataa gcgcagctgc
     8821 ccacagacag aatatcttct tttttatcat tgtttcctcc tgatcttgtg actgctgcgt
     8881 ttccttttat tttgacttta cctggatctg agactgcttt ttcatcagcc agtcataaaa
     8941 agttgcttta tcttctccga tgacacggtc tgtaagtaaa tatccatggg atggatctgt
     9001 aaaaatgatg gccatccccg gcagctttgc gatccttacc atttttctcc acggaatctt
     9061 atcgtaagta tttcccactt ttacctggat cccaacatca tccagacgga tctctgtgtc
     9121 ctccccggaa ccggatgcca gttttcctgc actgagataa acagccagcg gctgaaggat
     9181 tggaaaaagg cagcagccta aaaccataca gatgcggaat acggttcccg aagactccca
     9241 ttttgcacaa gtaagagcta taagagctgc tgtaaaaaca aggtttacca tgccggtcat
     9301 ggaatggtag atgaaataca tggacatttt ccagtattca cctctggttg ttttataacg
     9361 gtattggtac atgaattggt cccccttgaa tgtgcgtata cactcacgaa ccccgcgcat
     9421 tcgcactcta ctgcctgctg acgcaggcgg ttcgttcgtt aatggcttga aaaaaacaaa
     9481 tgtctgcttc gcagccgctt gtttttccct gtgctcatta cattatagca taaaagggga
     9541 tgtccacgac acccccttta tttttatatg tactacactt ttatcaaact tctgtcattc
     9601 tatcaatgtg caaatacact tggaagccat aagctgattc ctggtacgaa ggtgatcagc
     9661 agcagagtaa tgatcatgca cagatagaat ggtaatgttg cttttacaac cttttccatc
     9721 gggatctttg caacagcaga accgatgaaa agtacagcac ctacaggagg tgtcagaagt
     9781 ccgataccac agttaaggac aaccatgata ccaaactgga tcgggtcaat accaatagat
     9841 gttgcgatcg gcagaaggat aggagttgcg atcaggatga tcggagccat atccataatg
     9901 cagcctaata ccagaaggat caggtttaat aacagagcga tcaggattgg gttatcagtc
     9961 agtccaacaa tagcctgagc tgccagatcc ggtacgtgaa gtcttgtaag gcagtaaccg
    10021 aagatactgg aggttgcgat caggatcagt acgatagaaa gagtatctac acactcgccc
    10081 aggactctcc aaacaccttt ccagtcaaga cctttgtaaa taaatacgct gacgatcaag
    10141 ctgtagataa ctgcaactgc tgcagactca gtagctgtga acacaccgcc tacaacgccg
    10201 aacactacga tcagaacggc tgctaatgcc cagaaggaaa cgcttaactg ctttaacagg
    10261 ttcttcatgc tgaacttgtc gccctttgga tagttgcgct ttacagaaat aatgtaagaa
    10321 ccaaccatca gggatgctgc taaaagagct cccggaacat aacccgcaag gaataagctt
    10381 cctacggaaa taccacctgc tgtggtagca taaataacca tgttgtggct aggtggaacc
    10441 agaagtccct cacaggaaga agtaatagta actgcagtag agaagtcacc gtcatatccc
    10501 tggtcaacca tcatagggat cagaatagat cctaaggaag cagtatctgc agatgcagaa
    10561 ccggaaatac caccaaagaa ataagatgca acgatgttta ccattgccat accgcctcgc
    10621 atccagccta cgcaggcatt tgccagagcg atcagttttt cagaaatacc accggaaccc
    10681 atcaggcatc ccattgtgat aaagaaaggt actgccatta agctgaaaga gctgatacct
    10741 tttaccatct gctgacagat ggtagtcaga ggcagcccct gggccatcag acagaataca
    10801 gaagaaagag ctactgcgta cgcaatcggg aaacgcagca tgatcataag gaagaagctt
    10861 cccagtaata taagaattgc catgctattt acatccatac tacttctcct cctttacata
    10921 aaagcttctg atatgattgt acagtgcttc gatctcgaaa atgatcatcg caacacctgc
    10981 taacggaacc ggcagataca tccagaaacg ggatactcca ggcatactta cataagtacc
    11041 ctttgaaccg ataccggaag cataagtcca gcctacagtc agcatgatga cagccagtac
    11101 taaaacagct acatcagcaa ggatatccag ggatttaacc agcttctttg gcagatagct
    11161 gtcaagagcg gtcatacgga tatgagcacc gcggcggatt gccagagctg cggaaagcac
    11221 ggccatataa gccatacagg aaagaactac ttcttcagac catgccggat ctggaataaa
    11281 tgagatataa cgtcctgcaa cggacatact ggtaatagcg atatcaacta caagcaaaag
    11341 cttgcagata aataatacaa atttatatac cgcatcatac aaaggacgga tcttatcgat
    11401 cttttcaaaa attgaaggca ttcttcagtc ccctttctta attaaaagcg caggagagac
    11461 gcgctcccct gcgcctaata caccatttcc ctatttaact ggctgactca gattattttg
    11521 ccatatcaag gatctgctga tacagttctg cgttatcctt tgtagaggac tcgataatgt
    11581 ccttgcaagc ttcctgccat ggagtcttat cttctacttc tacaacgttt acgccatttg
    11641 cctttaactc atccagaact ttgttctctg cttcttcaga gatcttacgg ttgaattcag
    11701 atgcatactt tccagcttcg gtcagaacat cctgctgctc tggagttaat ttatcccaag
    11761 cgtcatctgt gatgataacc tggatagcac ctaaggtatg tccatcaagg atcaggttag
    11821 gagcaacttc tgggaatgcg ttggactgat agtttgcgat tggctgctca gcaccgtcaa
    11881 caacgccggt ctgcagtgcg gagtacagtt cgttgaagga aacaacagtt gcgtttgcgc
    11941 caagtccagc taccataccg ttcatgatcg ggtcgttaga aacacgaagc ttcattccct
    12001 ttaagtcttc cagaccattt actggcttaa cagtgaagaa atgacggaaa ccttcttctc
    12061 cgtagaacag acctcttacg cccagtccgt tgtcatgcgg ttccattaag aactctggag
    12121 ctaaatcgct gtctgcgaac ttccagaaat gttcacggtt ctggaatgta taaggtacag
    12181 acagaagtac ggacttctct gcgccgtagc ttgtcagagc gaatgcggag atacgggaca
    12241 tatcaatggt tccgccacca ccaagcatgg tatccagaac gtcgttctca gaaccaagaa
    12301 caccacttgc ctgcaggtct acagtgatag atccgccgga cagctcttct actttctctt
    12361 tgaatgcgct gtcagtctgt ccaacgatag tatccagtgg attaacctct gccattacca
    12421 gagtaactgc tgggcctact ggctcgccag cttctgcttt agcttcttcc ttggtctctt
    12481 cttttgcatc tgctgctgtg gttgcttcct ccttagctgc ctcagtagct gctgctgtag
    12541 ttgctgtatc tggggacttt aatccgcatc ctgccagtga tccagctacc atagctgtgc
    12601 aaagtgctgc acttaaccat ctttttttca ttttgtttac cctcctgtta atgcgttttt
    12661 cacatattgt ttatgttttt ttacgttttc tttatgctga tttcattata agggtaaaat
    12721 cttgttctga ccatggtaaa tttttgataa tcctagtatg attttgacct ataccgtaat
    12781 tttttatcta tttttcacgg aatacactgt ttctgtactc tgtaggactc tgccctgtga
    12841 tccttctaaa cacttttgta aaataattag agtcttcgta tcctactgac tttcctatat
    12901 ccttaatatt gacggtaggt tcttctaaca tttttttcgc catggaaaca cggtattctg
    12961 taagatacgc tgtaaaattc tgtccaaaac tctgcttgaa aagccggcag aaataaggct
    13021 ctgaataatt catcttgtca gccagatcct gtacagaaag atcatacatg taattttcac
    13081 ggatatagtc ccgtatgatc tctgttactt tattcatgcg cagattttca cctgcatcat
    13141 cttctgccag ttcttccaga tcgtcccgtt tttcttcctc ggatacctct tctgtctttt
    13201 ctttctgaaa atctttttct tctcccgcca ttcgcatggc ttcttccagt accagcatca
    13261 attctttttc atcccagggt tttaaaagat aatctaacgc cctgactgtg atcgccttct
    13321 tggcataggc aaattcatca aaagctgtaa gaaagataat acagcatttt ttatccttct
    13381 cccttatcct ctctgctgcc tggattccgt tgatacctgg catttcaata tccagtatgg
    13441 cgatctggat cttttctttt tcatatattt ccagtgcttc tcttccgttt tctgcctgga
    13501 agatctcaca ctgccctttc aggttctggc ttaatgtttt aaaaagaacc tttctctcga
    13561 tcatttcatc atctgcgatc agcagtcggt acatatactt cttccccctg tgtttcttct
    13621 ccctgcggta tcatcaattg tatcactgtt ccttttcctt cccggctgta gatccgcaga
    13681 tctccctttt ggtacatcat atggacacgc ttataaatat ttccaagccc gatcccgatc
    13741 tttgccgtgc ggtgttcttt cattgctgca gcaacttctt cgcaccgctc ctgactcata
    13801 ccaaggccgg tatctgccac tgaaaggatc agcatacggt cctttttcca gatccggata
    13861 tggagaatac ctcccgtttc ttttttagaa agaccatgaa ccaccgcatt ttctacaaga
    13921 ggctgcaggg acagggacgg aatgatcacg tctgccgcat ccgtctccag gctcagacgg
    13981 tatttgatcc tgcttccaaa acgcattttc tgaagataca tatagtcatt tacgatcttt
    14041 aactcccggt ctaatgttac agtctgctcc cttgttttca ggttatagcg gaacagattt
    14101 cccagactta agatcatctt ttccgtatcc acagcatctt ccagttttgc ggtacatgcg
    14161 atcatattca gggtattaaa gagaaaatgg ggattgatct gactttttaa cagttccagc
    14221 cttgcgcttc ctaactgttt ttccatttca ctgcgctcta attcctcttt gtggagcagc
    14281 tcctgcattt tgttattttc tttcatggtg gcaatatagc ccttggtact gtgtttcatt
    14341 acattgaaag ctgtgaccaa ttctcccatt tcatctttat tttctatgat caggtccggc
    14401 tcatcaaagt cattggcagc tatctttctg gtacttgcag ccaaagatac aatgggtttt
    14461 gcaacggaat ttgaaagaat tcttgtcagc cagatcgcta tgatcataag tgcgatggaa
    14521 atggccacaa taatatacgg aatattagaa aacattgctg cttctttctg atactcttct
    14581 gttccttctg ccatggttat ctgcaccaga cggccggtat atccctggag gtactcctgc
    14641 attttgtata cgtaatacag ttcgtctaca aattcactgc ctgttttatt tccttccgct
    14701 acgtcatcac gataaacttt gtaaccttca taggcatttc ttatgttcca tgtcctggca
    14761 ctgcgctctt ttccaatcct tctgtactca gaaggcagtg accacaggca acgttctgtt
    14821 cttacacatg ccaccctgta agtatcccgg ctttcctggt ctgcatttcg cacataatgc
    14881 tcaaaagctc tgatttcctg atccagtgcc tcctgaaagt cattgcaacg ggagttgtca
    14941 ttcaaaattc ctccaaagct tcccagggat acgttggtga tccacacatt aaaagaagcg
    15001 gaaagagcca tgatcaaaat gaccatggct gcaaaaattc ctatttttct ttttatggag
    15061 atcgtcatcc atagctttct cagagaagga cgcatgataa cctcctgttg taattcttat
    15121 gtcagtttct ttatgtcttt tgtagctttt tagcgttttt tattacggcc tctttttttg
    15181 gctgctctga gtgctgcttt ttttgctgca gctgaggttg gagccggggc tgcgggtgct
    15241 ggagatggag ctggctctgg ttttgtaact gctgaagcag gagttgttac tggctctgca
    15301 atcggatctg caggtgctaa aactggtact ggttcaggct ttgctgtttc cagtttcttt
    15361 ggttcttctt ttgcagttgc cagctttttc ggctcttctt tttttgtttc cagcttgtga
    15421 gcttctgtct tggcagttgc taacttcttt ggctcttcct ttttagtctc cagtttgtgg
    15481 ggctctgttt tagcggtcgc aagctttttt ggctcttctt tcttctctgc cgtcagtttt
    15541 ttgggttctg ttttctttgt agctaacttt ttcggctctt cttttttctc cgtagtcagc
    15601 ttcttcggtt cttccttctt tgttgccagc ttctttggct ctgccttctt tgtggtcagt
    15661 ctcttcgggc tttccttctt ctcctctggt gtgcaggaga ataccataaa gctcattggc
    15721 gggatatgga tctcaatgga gttctctctt tcatcccatt ctatcttctt ggatgctttc
    15781 acacggctgt tggtacggcc ttcgccgcca aattcttttg catcactgtt gaagatctct
    15841 ttatattttc ctgcaaatgg tacgcccagg cggaatttct catgatccat attatcaaag
    15901 ttacatacac agagcagagt ttcttctttg cctctgcgca ggaacatgat catgctttcc
    15961 tcatggtagc tgcagttgat ccactcaaag ccttccgtat caaagtcctg ctcatacagt
    16021 gccggatggg cctgatacag cttgtttaac tcacggacat aggtctgcat gttcttgtgc
    16081 agcggatatt ttaacagatc ccattccagg gacgcatttt cattccactc atcgatctga
    16141 ccatagtcct gtcccataaa taacagcttc ttgcctggat gggacatcat aaagccatag
    16201 gcagcccgca ggttttcagc ctttgcctct aaagtctctc ccggcatctt acccatcata
    16261 gagccttttc cgtgaacgac ttcgtcatgg gagaatacca ggacaaagtt ttcactgtag
    16321 gcataaagca tggagaacgt caactcgcca tagttatttt tacggaaata tggatcacag
    16381 cgcatgtagt tggtgaaatc gttcatccag cccatattcc acttataatc aaaaccaatt
    16441 ccgccgtctt ttggatcacc ggtgatcatt ggccatgcag tagactcctc tgcgatcagc
    16501 accgcaccgt tctttctgcc cttgaataca gtatttaagt gctttaagaa ctctacagct
    16561 tccagatttt catttccgcc gtagatattt gccacccatt caccgtcatt tttaccgtaa
    16621 tccagataca gcatggaagc caccgcgtcc atacggatgc cgtctgcgtg gtacttctca
    16681 gcccagaaca gtgcgttggc aatgaggaag ttagatactc ccggacggcc gtagttatag
    16741 atcagtgtac cccagtgcgg atgagcgccc tttcttgggt ctgcatgttc atagacgcag
    16801 gttccgtcaa agcatgccat tccgaaagca tctctcggga aatgggccgg tacccagtct
    16861 aagatcacac caatgccctg ttcatgcata tagtccatga agtacatgaa atcatccggt
    16921 gttccatagc ggctggtagg agcataataa ccagttacct gatatcccca ggaagcatct
    16981 aaaggatgtt ccatgatcgg cagcagttct acatgggtat agcccatatc ttttacatac
    17041 tcagccagct ttacagccag ttccctgtaa ttgtagaact cggagccaat gatatcttcc
    17101 ccgttttcgt ccttggcaat ctcttttcgc atccaggaac ctaaatggac ttcataaata
    17161 ttgaaaggct tgtcctttgt atcagaagca gcccgcttgt ccatccattt tttatctttc
    17221 cactggtaat tgttgatatc ccatacaatg gaagcattat ttggacgcag ctctgcataa
    17281 tttccatatg ggtctgcctt catcataggc agtcctgctc tggtcttgat ctcatatttg
    17341 taaaggcatc ctgctttcag gcccggaatg aacagctcaa atacgctggc atctccttcg
    17401 cctactttgc gcatctggtg gcgtctgccg tcccataagt taaagtcacc tactacactg
    17461 acacgcatag cacatggagc ccatactgca aaataaacgc cttctactcc gtcaatggtc
    17521 attggatgag cacccatttt ctcataaatg tcgtagtaga tacctgcacc gaatttcttt
    17581 aattcatctt ctgtaaactg tggtccgaaa gaatagggat cgtgaatctc ttcttcggtt
    17641 ccattatcat aagtgacaag taaggtataa tctgtcactg tttttcttgg gatcagtaca
    17701 gcataaaatc ctgcttcatc tgccagctcc atagggtatt ttttcccatt tcctgtaagc
    17761 tttacagtga tcgctgttgc ggtaggtata aatgcctgta tcaataagcc ttcttctgtt
    17821 gcatgtggtc ctaacattct gtgaggatcg gctgactctg aataggtcag ttcctcaatc
    17881 cccgcccagt ccataatatc atataatttc tgttccatct gcgtgccccc ttttagcaaa
    17941 aacaatgttg tttctattac agctgtttcg cccgttctga aatattctgt ctgaaattga
    18001 tcacatcatg ctcatattct tcatccataa gaggtgatga gatcataaaa tctgcacttg
    18061 ccttattgtt tgcaatggga atatcgtaaa cctgtgcaat acgcagcagt gcctttacat
    18121 ccggatcatg aggctgtgct gtcaatgggt cggagaagaa gatcacgaaa tcgatctgtc
    18181 cttctacgat cttggcgccg atctgctggt cgcctcccaa tgggccgctg ttatatccct
    18241 ttaccggaag tccggtataa tcggtgatca ttcttgcagt tgttccggtt ccgcataaga
    18301 aatgtttctc cagaatggat ttatgttcct tgcaccattc cattaattca tgctttttct
    18361 gatcatgggc gatcagggcg atatttttct gcttcttgat agtcattggg acaaattcca
    18421 ttgtatgcat acctccttat gttgcctgtc tcttgtgttg ctgtcctaaa tatatagtat
    18481 acaattgctc atttatgatg atgccgccgt caggcagcat acatgtttat tcaactaccg
    18541 gattggtaag tgtgccgatc ccttcaatgg tacattctac tacatctcca ggttttaaga
    18601 attttggcgg tgtaaagccc attcctgcgc ctgccggtgt gccggtggca atgatggttc
    18661 caggttttaa tgtcatgcct tttgaaagct cactgacgat gtggtcaata ccaaagatca
    18721 gcagttctgt gttgctgtcc tgtctcagtt ccccgttgac acgggactgg atagaaagct
    18781 ttggcggata tgggatatct tccactgtgg tgatacatgg tcccattggg aaaaatccgt
    18841 ctaaactctt tccaaaatac cactggttat gctgggtctg aagggttctg gcactgacgt
    18901 cattgataat ggtgtaacca aagatgtaat tttttacctc ttcttccggt acattttttg
    18961 cttcttttcc aataataaca gccaattcta cctcataatc cagatcggct acaatgtctg
    19021 aatggctttc aatgccttcg ccgtttgcca ccgcctcgtt tacacgcttg gagaagtaaa
    19081 cggcataggg acgttcgcct ccaaatgcct ctttcttgta gcgggcagac tcctctgcgt
    19141 gagccatata gttgataccc aggcagatca catcctgttt cggatttggg atcggcgcca
    19201 gaaactttac ttcttcaata ggtgccgctc ctctgatctt ataagggtcc tgaccggaca
    19261 catattcaag cagctgtttt tctgactcgc tgatcccctc gatcagttcc tgcatggtct
    19321 tataatccat atccagggag cgaagaggat agacccattt tccgtctctg ctgatcacgc
    19381 ccagaccgcc tttatgttcc acttcatatg taacaagcct cataacagcc tccttatttc
    19441 atttagcaat ttcttaatgc ttactttatc acatttttcg gatattttcc accttgccac
    19501 aaatgtttta cataatctta gaaaatttag cggacaattc agacgtcatt taagcggttc
    19561 ctgcgctcat cattttgtag atctgaataa ctgccgttgg tgccagcgca cctaaaacca
    19621 ctgccagtaa ctgtgagcct gtcagatccc ctgcggcaaa tacatcctga agctgaggca
    19681 caaaaagcac tgcacacata aggactacac ctacaaacaa tgccataaca caccagagat
    19741 tgcttcctat tcccaatgaa agcaggcttt ttcttcccct gcagttaaaa ccatggaaca
    19801 gtcttgccag agtcagagtg gaaaatgcca tagtactggc aatggcttcc ccgccctggg
    19861 aatatcccat ttcccatgcc cacatggttg ctgcaccaat aagaaaaccc tggaataaga
    19921 tcttccctgc aaatggaaaa gaaaggatcc cttcttttgg gtctctggga ggctggttta
    19981 aaagatcccg gtctgccggt tccataccta tggcaatggc aggaagggag tctgtcagca
    20041 ggttgataaa cagcagatgc accggtgcaa aaggcaatgg aagcgccttg atagatgtat
    20101 acagtacaca aaggatccct gccatatttc cggacaacaa aaactggatg gaatttttaa
    20161 tattccgata tacatttctt ccatttgcca ctgccttgat aatcgtagca aaattgtcat
    20221 ctgttaagat catggcagcg gcatcccttg aaacttctgt gcctgctttt cccatagcga
    20281 tccctatgtc cgcctttttt aaggcaggag cgtcatttac cccgtctcct gtcatagctg
    20341 taatgtgccc ttttttctgc catgcttcca caatgcggat cttatgttcc ggagacaccc
    20401 tggcatatac ggagatcttt tccagtcttt cctgcagctg ctgatcgctc atctgatcca
    20461 gatctgctcc tgtaacagcc agatcaccag ctgtaaaaat accaatctcc gaggcgatcg
    20521 ctgccgcagt ctctttatga tccccggtga tcatgaccgt gcggatccct gcttttttag
    20581 catctgccac tgccatttta gactccggcc ttggtggatc tgtcatagca gccagaccca
    20641 gaaagatcat attttccgcc tttgcatccc tgatttcttc ctcttttaat ggtttacatg
    20701 ccagtgccag aacccgcatt ccctttttgg accatagacc gttctggcga aggatccgct
    20761 ctttatcaga aaaggaaaag ggacgtcccc aggaagcagt ctcttcttcc tgaaaagagg
    20821 gattgactgc cagactgcat ttagacagta aaacgtctac cgcccctttt acaaaaagga
    20881 tcttttctcc ccctttgctg catatggcac ccatatattt tttatcagag ttaaaaggga
    20941 tctccgaaat acgacgccac tgcctgcgaa gaagtccggg atcttttcct gcttcttctg
    21001 ccatatgtaa aagagcctgc tctaagggat cttctttttc cccggtctcc tctccggaag
    21061 cattatttgc aagaatgact gatcgcagga aaagagaaag atccgggtgg tgggaatatt
    21121 tttttaactg ccctgtatcc atgaccttcc catttatgca aacagcctct gcccgcagac
    21181 tgttttgggt cagtgtccct gttttatcag aacagatcac agatacacag cctaaacttt
    21241 ccactgcttt caggtccttt atgactgcat gctcttttgc cattttctgg gttcccattg
    21301 cctggacaat ggtcacaatg gaaccaagag cctccgggat ggctgcaacc gccagcgcta
    21361 ccgcaaacat cagggcatct aatacaggct ccctgcgata caggctgatc agaaagatca
    21421 caccgcagat ccccataatt cctaatgcca gatggccgga aaaccggtct aaactggcct
    21481 gcaaaggtgt tttcttttcc actgtgcggt tcatcaggga agcaatggtc ccgatctggg
    21541 tatccattcc tgtttccgtc actaccgcag ttccccttcc gccagtgatc agagcaccgg
    21601 aaaagaccat attgctctga tctcccggag ataaaggcgt ttttcctgcc ggcagttttc
    21661 cctctctttt ttccacattt aaagcttctc ctgtaagaga actttcgttg caggtaaggc
    21721 tgcaggactg aaggatccgg ccgtctgcag gtaccattcc tcctgtttcc aaaaccagga
    21781 tatcccctgg aaccaccctg gaagatggcg ccgtacaaac gcgtccttcc cggatcagaa
    21841 gtgtttccgg agaagcaagt cgctttaagc tttccaggga ttttcgtgct ttctgatgtt
    21901 ccacagtccc taaaatggca tttaacaaaa ggacggaaaa gatcacaaga gcgctttccg
    21961 gatctccggt aaccatggat acccccgcag ccccaatgag gatcatgacc agaagatctt
    22021 taaactgtcc taaaaatacc tgccaccagg gcatttcttc ctgttctttt aaaacatttt
    22081 ccccatatgt ttccagcctt ctttctgcct ctttctcatt tagcccggtc ctgttggtgc
    22141 caaggcgctt taaaacttct tcctgggtca gaccatacca ttgtgccatt cctgcctcct
    22201 atagactctg tatagacttc atatagaaaa aaatgaggat gccctgaccc gtctggcctt
    22261 tacagcatcc tcatctgaat gtatatgtat ttctctttta tctttatgca ggaaaaaaca
    22321 ggcttatgac ctgcctccga atatcatctc aaaagcaact ctgtatccta aaggaaggga
    22381 agcttcttct acatattcct ctcttgtatg agcgccggct ccactatagc agcccacaca
    22441 tacacttggg attcccatag aaagcgggat attgcagtct gtagaaccag aaccaactcc
    22501 agggcagatt cctgttactg tctctaccgc tgcttttgca cgttcaagaa ggcgtgcctg
    22561 acgttcttta tctacatcac cggagcatgg acggatgccc aggatctcat aagtaatatc
    22621 aagaccggct gcctttgcct catttactgc tgcctcaaat ttttcttcca tataagccag
    22681 atttactttt ctgtcagagc ggaactcgta tagcatggaa gcttcctggg caatagtatt
    22741 aacagaagta ccgccggaaa tagtacccac gttgtaagtg gtctttccgc cttccggcac
    22801 cttgatctga tacagctttt caatgatacc tgccagtttt tcaatggcat tatcattgcc
    22861 aaaattgcca aaagaatgtc cgccctgagt gcgtaccgtc actttataac ggttagatcc
    22921 cactgcttca tctaccagat gctccagact gccgtcgaag gtgcagaaag tgctcatacg
    22981 gctgccgtaa tcttcgcaga tctttttaac gcctttcagg tttccaagac cttcttctcc
    23041 tgaattgcat acaaagatca gtccaggttc tgtttccggc cggatctggt taagtttgcc
    23101 ttcttttatc ataagagcag tatattttgc tgccagaaga acacatacaa ggcaggcagt
    23161 atcatcacct actcctggac agcagatacg tccattttcc acctttaaag gaagttccgt
    23221 ctcatctgga aaaactacgt ctgtatgggc catataaaca tccacattat cccttaaagc
    23281 agctggataa actacattta atgcctcatc aatatagact ccctctgccc cctgttcctt
    23341 taaccagtta cagcaaaact gggctctttt ttcctcatga ttagatgggg aagggatctt
    23401 cgcaatggta agaagcaggt catatgcctc tttttcatgt tccttgataa aacgttccat
    23461 agcttcattt aacatgttct gttccttctt tccggttcgc tttcggacct ttgctttgtg
    23521 tttttttctt ttccgggcat ttaagtcctg caaatacctg gatggatctt gcaaggacaa
    23581 gagcctcttt ctggtcatcc agatgtcttt ccttacctga cggcaaaaag ccgttcatac
    23641 tgtcttcagc tgtatcaagg acctgatgcc agatatatcc tgacggcaga ttgggaagtg
    23701 caaatgcatg tggctcccag tgcatgttgt aagcagtata gaaataaggt tcctcgctgc
    23761 cgtctgtatt ttctccatag gaagcgctgt aaaatacgcc taactggcgg ctgtagcgct
    23821 caaagtccgg acaccatgcc ttttccccgt ggaaagacac atccggcagt cctactgact
    23881 tatgatccat taaagtaggt tctgtactgc ggtggaacag gctgtgcttt ttgcggaatg
    23941 cgatcaggtt tttcgtaaac tcataaagcc ccttgtgaga gtctaataaa ttccagttta
    24001 accaggagat ctcattgtcc tggcaataag cattattatt tcccttctgg gtacggccaa
    24061 attcatcacc agccataagt aacggggttc cctggcttaa gaaaagcatg gcaagagcat
    24121 ttttaagctg acgttttcgt aagccaatga tctttttctt tcgcaccggt ccttctgcgc
    24181 cacagttcca ggactggttg taatcggtac cgtcatggtt ttcctcgcca ttgtcctcgt
    24241 tgtgcttatg atcgtaagaa accatatcca ttaaagtaaa gccgttggta ttcgccatgt
    24301 aattaataga agccactgca tccggattgt cctttacatg aagagccacc tggtttaaca
    24361 taccttcatc gccctttaag aagctgcgca tatccagcat aaatcctgtt tcgtaagcag
    24421 ataaacggcg tccggtctct ttcttttctt ctttggttct gttgcccctg tctgtttccg
    24481 tcgcagttct cacttcatcc cagttcttcg cccaaagttt cagatcagaa agatatgggt
    24541 ctttcacaat agtttccaaa ggggcataac ctactaaatg gataccatct acatgatact
    24601 gggcagccca gaagcggagt acatctacca cataggaagg agactgtgtc ccgtcaaagt
    24661 ataattcaac gaccagctcc agaccttctt tatgtaatgc cttcaccaga gtcttaaact
    24721 cttttaccgg atcctttttc tgtcccggcg ctgcgcctgc ataggctgct tttggcgcaa
    24781 agctgtgtcc acagcagtac ccccagtaat tcacctttcc gggagcgtct tccatgatct
    24841 cgtcaaattc tgcaggcggc atcatttcta ctgtagtaat tcccagttct ttcatatagg
    24901 ggatcttatc aatgaccgcc ccaaaggttc cccttttcca gctttcccct ttaaatgaga
    24961 catgttttga aaaacctctc gggtgtatct tgtatacaat acactcattg gctgggatct
    25021 caggccgctt gtcatcttcc cagtcaaagg cttctttcac tgcttcaaaa ggtgtgtgaa
    25081 gtgcctgccc tgctgccttc tcgtctcccc atttttcccg tcctgtaaaa caggttccaa
    25141 aaggatcaga gatctctttt ccatcttcct caaaagcgta ttccaatccg gtaaaatccc
    25201 cctttaccgt catgaaatgc acattcccca tacgggcatt tttcggaaat gcaagtctgg
    25261 ccatccgttt tctgcttccc ttttcaaaaa gcagcaatgc cggacttttc tcagaaacaa
    25321 atgctacatc aaatcccccc ggagtctttg tcactcccag gggatagatt tttctctcat
    25381 ttgtctctat gctatactct cttttcatat attgccgcct ttcaatccca tttcctttat
    25441 ttgcactgtc aaatcccaaa tcctgctgcg cggcattgcc gcaaagaagc tgcctttcag
    25501 attttggcct atttctggct ttcaatctgt tccaccagat ccgtcagata catccaacgg
    25561 tccatttttt cctctaactg tgcctctttt tctgtctttt ctttcatcag ttcattgaga
    25621 cgggtataat tgctggcaga tttctcgatc tctgtttcca gggctgcaat ggcctcttcc
    25681 agacctgcaa tatccccttc aatggtctcc cattctctct gctctttgta gcttaaacgc
    25741 agctttttct gggcaccgga agtctctttc cagttatttt tgttcactgc ctttgcattt
    25801 gcagcgtttt cttcgtcaat atctttatcg gatgcctttc cagaacctgc atttccggct
    25861 ccagccccat ttccactgat ctgtggatga cgctcattcc atgcacgctg ataatcggta
    25921 aaaccaccct catactgctg caccacacca tttccctcaa aagcgaaaat gcggtttacc
    25981 acacggtcca ggaaataacg gtcatgggat acagtgatca caataccagg gaatgtatcc
    26041 agataatctt ccagtaagat cagcgtctgg atgtccaggt cattggtcgg ctcgtccagg
    26101 atcagtacat ttggtgcttc cataaggata cgcagaaggt acagacggcg tttttcacct
    26161 ccggacagct ttccaatggt tgtatactgc atactggaag ggaacaggaa acgctctaac
    26221 atctggctgg cgctgatgct gccgtctttt gtctttacgt actctgctgc attgcggata
    26281 tagtcgatca ccttcaggct ttcgtccata gcctcatttt cctgggagaa ataacccagc
    26341 tttacagtct ggccgatctc tactgtaccg gagtccggct gtacccagcc tgcaatgatc
    26401 ttcatcaaag tagactttcc gctgccgttc tgaccgatga tacctacacg gtcatttttc
    26461 aggaagatat aggtgaaatc tttcatcagc accttatcac catatgattt gcagatgtcc
    26521 ttcagctcta cggtagtacg tcccaaacgg ctgccaatgg actccagctg tacatttctg
    26581 tcatactccg gaccttcctg gtcacgaagc tgctcatacc tctggatatg ggctttctgc
    26641 ttggtagaac gggctctggc accacgctgc atccaggcga tctctgtgcg caggatggac
    26701 tggcgcttgc gctctgctgc ctctgccatg cttaaacgct ctgcctttaa ttccagatat
    26761 ttctcataac cgccctgata actgtacagt tttcccttgt ccagctctac aatacggttg
    26821 gtaacgctgt ccaggaaata acggtcatgg gtgatcatta aaagagcgcc cttaaatgcc
    26881 tttaaataac cttccagcca gtcagccatc tggctgtcca ggtggttggt aggctcgtcc
    26941 aggatcagca gatctgctgt acttaaaaga acgcttgcaa gagccacacg ttttctctgt
    27001 ccgccggaaa gagtctccac tttcgcgtca aaatcttcga tctccagacg gtttaacatg
    27061 gtcttggcag aagcctttaa ctcttccaca gagctgaaat gaggctcgtt tttattgtcc
    27121 cttacaatac tgtcaagaat ggtatctcca ggggtaaatt ccgggatctg gggcagatag
    27181 cggatataca ggctgcggcc ttttacaacc gtaccgccat ccggctcctc cagacctgca
    27241 gcgatccgca gcaaggtaga tttccctgtg ccgttgatac cgatgagacc cactttttct
    27301 ccttcgttga tagaaaaagc agtgtcgttg aacagcaggc gttctgtata ggattttgtt
    27361 aaatgttcga tggtaagtaa attcatattg ttcattttcc tcttattcat acatcttaga
    27421 aaagtataac acaaaggaaa aaagggcaca acaaaacacc ggatgttttt tatccacatc
    27481 cggtgctaag attttctgct tttttcacat atccgacatt aatctacatt tccatataat
    27541 tcatggacct tactcataag aacctttccc acactgttac gtggaatttc ttctacgatc
    27601 tccacatact taggctgttt gatctttgcc agatttttac tacagtattc ttttatatct
    27661 tctgctgtag ccttagagcc ttctcttaag actacaatgg cttttcctac ttcacctttt
    27721 cgcttatccg gtacaccgat cacacagact tcgtgaatac caggacactc cttcatgata
    27781 ttttcaatct ctaccggata aatattttcg ccatggctga taaacatgtt ctttttacgg
    27841 cctacaatgt aataatatcc ttcttcatcc tgctgggcaa tatcaccgga atgcatccag
    27901 ccattctgta atgccagttc tgtctcctcc gggtcgcccc agtagccgga aaacatggct
    27961 gcagagcgga taaacagttc tcctttttca ccctttggaa catcttcgcc tttttcattt
    28021 acaatgcgaa gcttcacata aaccattggt ttgcctacag aaaggcgttt ttcctcaata
    28081 tcctttacgc tcatggaatt aataggcatt gcaagaatgc caggtcctac ttctgtcatt
    28141 ccatatccat tgcaaaatcg aacacccttt ttccagtaag ctctcatgat ctccaaagaa
    28201 gcaggcgctg cgccacagcg gatacgtccg atctcagaaa aatcagctgt ttcaaatgcc
    28261 ggatcttcca tcatatactg gaagatagta ggaactccta acaggaatgt aagatgttcc
    28321 tgcttcatga tctggaatac atcctttttg tcaaattttc tggtcatgac cattctggca
    28381 cctacatata ataatggcat cagaaggctg ttccaggctg ctgcgtggaa taaaggcaga
    28441 catacataaa ctacatctct gttgttaacg ccataagctg tcacctgatt gatcacattg
    28501 taataaagag cccgatagga gatcatcgct gctttaggga tacctgtggt tccgcctgta
    28561 tgtacaagca ttgctatgtc ttcaaactga tgctcaggaa gagggcgatc ttcatcagaa
    28621 tactctaaga cctcctgata acaggccaga tctccttcct gctttttgtc tcctatggaa
    28681 ataaatgcta actctgagaa acgttttttc aaagcagctg ctttttcttt aaactcttcc
    28741 tcataaagca atacagacgg cttttctttt aagatcagct ctgaaagttc tctctctgtc
    28801 agctttacat tataagaagt cataatatat ccaatgcgga caatggcata gaacatatca
    28861 atatgctgca cacagtttcc ggcgcagatc gccactctat cccctttttt tattttcgcc
    28921 ttatctgtca ggaaattcgc aagttttctg gaacgtactt caaaatcctg ataataataa
    28981 cgaatgcctg tagtatagtc cagcaaagtt tcacttttgc cgtcccgttc taaccggctc
    29041 ataaaaaagt cccggatgat ctcattatcg ttcatcatgt atatccccct ttagatctca
    29101 ctgtcggaaa cgtctttgat cttgttataa taagccagat aatctggtga gatcttttct
    29161 ttttccaaca tagtcaatac cgttaatatt acaacacttg ccggtactgc agcccagaaa
    29221 ggctgtattc caaaaggatt tccgctaaag tgccatacac ctgctaaaat gccgccgatc
    29281 aggatggacc agaatgcagc ccggttgctt acctgcttcc ataccagtgc aatgatcagc
    29341 accacacctg ctacggaacg gatctggaat gctcctaata tctgtgtcag gatagaagaa
    29401 gtagtaagtc caaaacctgt ggctactaag gccattacaa caatggagcc cttagaaaac
    29461 cggatctgtt ctttttcact tgcctgaggc cgcaccagca tgtaaaagtc tcttgtcagt
    29521 gtagcagata atgataaaag aagtccgggg ccagtagaaa gaatggctgc taaaactgcc
    29581 atgctgacaa taccgctgaa taccgggtga acactttctg ccatggtata gatgatattt
    29641 ccagcctggg catcaggcat tgtcacaaaa cccgcaatac cgatcagagc cggcatcagt
    29701 gcaaatggaa taaccagaat accagaccag acaataccac tttttgctgt tttcaaagac
    29761 tctgctccta aacagcagtt tacaactgta gaagaaagga tcatggaaaa agcagctcct
    29821 aacacctggg ccgctaccgt tattttatcc ggataagtaa cggtaaaata atgagccggt
    29881 gtatttgcca tcatatatgc cagacctccg gcttttccta cagcaaggaa gctgactaca
    29941 aacattcctg caaacatcac aagagaatgg atcacattca ttcctgcaat gccttttaaa
    30001 ccatgaagag ccatataggc aaataaaata cctacgatcc aggctaaaac agccttattt
    30061 acacctaaca tggatgagag aagggcagcc gcggcaatgg gctgctggga aaatatgatc
    30121 ccatatacaa acatgatgat gactaacacc accagacgca ctctcttatc aaagcgaaag
    30181 gcaaatgcct ccggtacaga cattactcca aaatgacctg ctctgcgata aaatttcgcc
    30241 gtacagaaca caaataccaa aactcccagt accattcccc agttggtcca tacagaagac
    30301 aagcccattt taaaggcact ggcagcattt cctactgtag aacttcctgc aaccatttcc
    30361 ccaaacatta aagctacggt aagacctatt cccaaatgct tttttgctac aaaaaaatgc
    30421 tgtatggaat tgtccccttt tccattcatg atctttcccg cggctgtggt aatgataaaa
    30481 tacgcaacca ccataaataa ttctatcata tccctctccc atcttagctg atgcagtaag
    30541 gcttattcag ccttactgct ccgtatccgt tttgtaagtt ccgggattat ctcaaataag
    30601 tcccctacga tcccgtaatc tgcaatgtta aagatcggtg catctggatc tttattaata
    30661 gcaatgatca catccgaaga tgacatgcct gccacatgct ggatggcacc ggaaatacca
    30721 catgcaatat agatctttgg tccaacgctc tttccagtct gtccaacctg gtgtaaagaa
    30781 gagatccagc cggaatctac agctgctctg gaagcaccga tctgggcacc gatctcatct
    30841 gccagctctt ttaatacttc aaaaccttct ggtccgccaa cgcctcggcc acctgaaaca
    30901 atgatctctg cttcttccag gttcacatct gctccgccag cctctgtaat aacctctaca
    30961 atgcgggtac ggatctcatc tggtccaaat tccacggttt catggatgat ctcgccctta
    31021 cgtcctgtat cttcctctgg ctttttaaat acgcctggac gtacagtacc gatctgtggt
    31081 ctgtgatccg ggcagagaat ggttgccatc aggttaccac caaaagcagg tctggtccat
    31141 tttacaagac gggtctctga atctactccc agctctgtac agtctgcagt aagtcctgtc
    31201 tgcatacggc tggatactct tggacctaag tcacgtccat tattggtagc accgataagc
    31261 agcactgccg gattatactt cttaattaag gtacacattg cctttgtgta accatctgtg
    31321 gaataatcct ttaattcctt ctggtctact acaatggctt cgtctgcacc atagtggata
    31381 gccatttttg caaaatcttc gatctcattt cccgggataa tggcaactaa tttctctccc
    31441 aggtcatctg ccatttttct tccctgtcca agaagctcca ggctgactcc ttttattttt
    31501 cctctgacta cttctataaa tacccagaca tttctagcat cactcataag tctgtcccct
    31561 cctaaaatgt gatcttagct tcagacagca gctttaataa attaccggct tgctctcctg
    31621 catcttttcc ttctacacgt actcctgtag aatgaacagg tggaacaaaa gtctttttaa
    31681 ctttggtagg agaacccttt aagccaagtc ttgccgggtc taattcaggc aggtccgcag
    31741 ctgttaatgt acgcacttcc atacggtttg cctgcatttt cctgcggata tttggatatc
    31801 tgggctcatt taaggatttt acagctgtaa gaagggcagg aagttttacc tctatctttt
    31861 catagcagtc ttcctgctct tttgttacag ttaaagttgt gtcatttaat tcaatatctg
    31921 cagcataggt cacctgcggg atacctaaat gttctgcaat ttccgggcca acctgtgcgg
    31981 tatcgccgtc aattgcctgt tttccacata agatcagatc aaattcacct aatgcgcgga
    32041 tagcagcaga cagtgtatag ctggtagcca gtgtgtcagc accaccaaaa gcacggtcac
    32101 tgaccagtac catgttgtca gcacccattg cgtagcattc tcttaaagct tctgctgcct
    32161 ggcctggtcc catagaaagg acagttacct ctgccccata tttatctttt aactgcaaag
    32221 cagcttccag ggcattttta tcaaatggat ttacaatact tggaactcca ttacgaatca
    32281 atgtatttgt caccggatcg atctttattt ctgttgtatc cggaacctgt tttacacaaa
    32341 caagaatctt caatctgttc acctctcctt tgctctttat cagcttttta tcagcatttt
    32401 ccaataatgg tcttgccaat gatctgacgc tgtacctcat tggttccctc aaaaatacga
    32461 tatactctta catcacggag cattttttca attgggtaat ccttagaata tccatagccg
    32521 cctaagatct gaagtgcttc atctacgatc tcattcacag cctctgaaac ataggtctta
    32581 ctgatagagc ccaggcagga aataggcttt ccttcatcta ctacctttgc tgcatacatc
    32641 atcatagctc tggctgtctg aagcttgatc tccatatcag cgatcttaaa ctgaatgccc
    32701 tgcagctttc caatcggttt gccatatggg catctttctc tggtgtactg agctgcgatc
    32761 tccagtgcct tctgtgcagt accgatcagt ggagctacgc agataggacg ggatttctcc
    32821 agagccttca taacgatctt aaagccatcc ccttcttttc cgatgatatt ttcagctgga
    32881 acatgtacat cctggaaaat aacatctgtt gtattagaaa gacggatacc catcttgttt
    32941 tcttcttttc caacgctgac gcctggtgta ttacgttcaa cgataaagca gcttaaacct
    33001 ttgtgtccca gttccgggtt agtagaagct actactacat atacatttgc cagcccgccg
    33061 tttgtaataa aggttttggt accattgata atgtactcat ctcctacacg gactgccttg
    33121 gtcttggttc ctgctacatc agaaccagac tgaggttctg tgatacacat agcggagatc
    33181 tcaccattta acagtctttc tgccatagcc cgcttctgct cttcgcttcc gcctgcaagc
    33241 acctgcttaa atccaacagt ggctgcaccg aagcttactg caaaaccaca gtctacacgg
    33301 ccaaactctt cacgaagaag agataaagtc tgcagatcaa atcccggacc accgtactct
    33361 gccgggatat ccattcccca aagtccgatc tctcccattt ttttaaatac ttccattggg
    33421 aatctggaaa ctccatcaca ctcctgtacc agtggtgcca gttcattttc aaggaattta
    33481 cgaacaagcc ctgtaatatc tttctgctct gcagtcatta attgccattc catatatttt
    33541 cctccattat ctataagaga tgaagttatc ttcctttaaa atccggtttt ctcttttcaa
    33601 aaaaggcatg tattgcttct gcatgatctt ctgacttgca cagctgatac tggtattcac
    33661 cttcattttc catagcatct tccagtccct gatataactg tctgtttaca agacgtttca
    33721 tgcgttcata agcaagtgcc gggccttttg ccagcttttt cgcggtcttc atgaccattt
    33781 cttccagatc ttcaggagca gcggcggctg tgataatgcc ccagtctgcc gcctgttctc
    33841 ctgtaaaccg gtggcctaac atcagcagtt cttttgcctt tgctgctccc aggttttttg
    33901 taagcataag agaggaaccc atatccggaa taaagccaat gttcacaaat gcaaatacaa
    33961 acttggatgc cagctgtgca taggaaaaat cacatgcaag agcaatgctt aagccgccgc
    34021 ctgctactgc accttccagc catgctatta ccggctttgc gttatttacg atagaaagga
    34081 tcacatcatt gttgcatctt aaaccaggtc ttgtttcgca gcagttattg tcaactttat
    34141 atttcatgcc ctttacatca ccgccgccac agaaattccc ctgtgcgccg cggatcacaa
    34201 taacacggac ctccggtgaa tcactgcatg cgtttaatat ctttgtaaga gactgtgccc
    34261 cttccattgt aagagcgttc agtttttcag ggttgttcat ggtgatcatt ccgatcccat
    34321 cttttaactc aaaatccgca tactttaatt ccggtacttc cattcctgct cctcttttct
    34381 tctgttaagt attatttttt catttcttcc ttgaattctt tcatcatctg gtcgatctct
    34441 tcatcactgt agcctaatcc acctaagacc tcatggttat gctgtcctaa ggtagctgcc
    34501 ttgtggatct caccaggaac atcagagaag aatgcattgc agcctcttgt ctggtaagtt
    34561 ggctgcttaa tatcatcagg tgtaggtgcc tgtacgatat agcccatatg ttttacctga
    34621 ggatcatcta ctacctgctg gtcattatta accaggcaat atgccatgtg gcattcatcc
    34681 attctcacgc atgcttcttt taaggatggg aagctgctaa gccatgcttc gatctctgca
    34741 atgaccttaa ccatgttttt ggtgcgggca ccgatggtat tatactccgg atcagtaaga
    34801 taatccggtc tgttcattgc ctttgcaact gcttcccatg cttttgcaga tggtgcacag
    34861 ataataacgc tttctccctt atctgtattg aagcagccaa atgggcataa gccactgtgg
    34921 tggttaccgt ttggtttaca taacactccg tcatttaagc ggtcaatggt actgttcaaa
    34981 tggatcatgc cctgtaacag ggaaacatca atgtactggc cttttcctgt tcttctctga
    35041 taatgaagag cagtcagaac agaagcaaat acattaaatc caccaaagta atcagctaaa
    35101 gcaataccat gttttaatgg ctgtccaccc ttttcaccgg taatactcat cataccggaa
    35161 agtgcctgtc ccatcatatc aaatccaggt ttctgggaat aagggccggt ctgtccaaaa
    35221 gcagttaaag agcaatagat cagtgacgga ttgatctctt ttaaggtttc ataatccagt
    35281 cccagacgtt tcataacacc tggacgattg ctttcgataa gaacatctgc atcttttgct
    35341 aaagccttaa cgatttctat tgctcttgga tcttttaaat ttaaggtaat ggacttttta
    35401 cctctgttta cccatgcatg agtcagactc tttccttcca ggatcggtac ataggttctt
    35461 tccggtccac cgttcatcga ttcgatctta ataacctgtg cgccataatc agacatttcc
    35521 gcacttgcta caggaccagc cgcattggta gtaaaatcaa gaacttttac atcatctaac
    35581 attccagcca ttttcattta cctctctttt ctttctcttt taacgttcct catgctttta
    35641 cagcataccg atcatcttcc agtaaggaac actgataagg caggcaacca ggtttgcagc
    35701 tgcccagata attgcgtaag gtacgcagtt cttattgtga tctaccatat cgcctacaat
    35761 accgatagca gatacatatg gcaggttctg gaaatcaaag aagaatacgt ttacagatac
    35821 atatgcgata aagcatggga taaatggatg catacccgcc tgaactgcta aaggagcaag
    35881 gagtacggaa tatacagtca taacacctaa ccaggaaata acgataaatc ttgtaaggta
    35941 taccagtaaa atgaagatca gtacgaataa tacaggattg ctaaggaatg gatttaaaac
    36001 aggtcctaaa acgccgccga tccattttgt aataccccag gtggtcagtg cagaaccaat
    36061 ctcatacata cagccgatgt aaatcaggat atcccagttt acttttgcgc ggaactcttt
    36121 cacgcccata accttaaggc ccataagagc acatacacct aacatagcaa cgattgcaga
    36181 gctgatgcca tgctgacgtt cagtcatcca taataccaag cagattgcta aaactgccat
    36241 tgtgatcttc tcacccttgt tccaggttcc cagttcaacg atcttgtttg caataaagtt
    36301 tttgtttaaa ttaatctttt ctgttggttt gtagtagaaa tatgtagcaa tggtagtaag
    36361 tcctaaagca acgatagacc acggaagtgc ccagataaac cagtctgtcc atgtaacatc
    36421 agagtaacct tctggaagga acttcagcat ggagtaacac ataaaggatg cacttaagaa
    36481 tacatggctt gtacagttaa aaccagtata taaagctgca aatagacctg ccattggctt
    36541 tgtctggttt tccagaccca tttcatgtcc aatctccttg gtaacagggc ctgcaactgc
    36601 cagtcttgct gtaccacttg gaatcaatgg agaaataaca acaccagaaa gagtaatacc
    36661 taaaaactgc cccagatagg ttcccgggaa tgctttcata actaacaaag tcagtctctt
    36721 taacagtccg ctgtgagcta ctgctgcacc taataccaga gcgccaacta acagccaata
    36781 gtttgtgccg ctgaacgggc tgaatgcctg agcaaaagta agactcttgg tagctgcaca
    36841 taagcagcag aacatcagca gaatgatgta ctcaggaagt actcgaaaga tcatccagat
    36901 aagagcacat actaaaagtc ccattccgat catggacttc tgtgggtcta atccttctgc
    36961 aacaaccaga tttgccggtg gagccatcac actgagccct aagcatgctg cgataccaat
    37021 gattccccca ataatttgtt tcttcgacat aatgaacccc cttaatttgt taatttttta
    37081 actattaagt gtaaataata aaatggctgg ctttcaaccg attctctgcg ctgtggttta
    37141 tttttctgcc agtcatttca tctgacttaa ctataataaa aaaaagattg ataatctaat
    37201 accaatcttt ttatcctaaa tatagcttac aagttatgtt ttttcctaaa agtactcaag
    37261 tatatattcc ttatgcgtaa tacaattatt gtggaataca cattgcttct cttccatcag
    37321 gcgcttttcc agatctgcaa agtagtttaa agcatattcc ctgaacatct ggtttctgcg
    37381 gctttcaaag tttttcttcg gccagcataa ctttaacaga cggtaatttc ccggatctgc
    37441 aataaatgca taggtaagat cctcatgttc ctttaacgtg tcttttaatg aatcataagg
    37501 gatgatcgat ataccttcat tttctgatat ggccctgaaa atacgctcca tttcgctgta
    37561 ataagcacag aatttcggta caaagccagc tgcatgacat aagcttacca tcagggtgtt
    37621 gctgctggag cctctccagc ctgcaataaa cggctcttct cttaattctg acatatgcaa
    37681 aagctttttc tttgctaatg gatggctgcg gtttaataca atgacgattt cttcccgcat
    37741 aagatactca aattccacat tctcatatgt gttcatagac gcggaaatgt ggaaatcata
    37801 ttctgtcatc agttttctct gcatcaaaga aaaattagcc acatccaaaa tgatatttgg
    37861 atgcagcttg cagaataagt catacatagg tgggaaaaag cactgtcctg cctttacgat
    37921 cagggaaatg gtaccatttt cagaatccgg tgttacagaa agtccatcta ccgctgaatc
    37981 gatgagattt aagcctttgc tcaccttatc gtaaaattct tttccctgtt cgtttaagac
    38041 aatacgcttt tttacacggt caaacaggac tactcccagt tcctgttcca ggttttttat
    38101 ggttttagaa agagaaggct gcgatacatg caattcctca gccaccttgg taacactttc
    38161 tcttcttgca acttcacaaa aatatctcag ctgtaaaagc tccattcagt cttctccttc
    38221 tgttttccca tatttcccgg actgactggt tttaagggct tatgcctgta aagttcccag
    38281 tctcttataa acagcctctg cctcattgat catatcgtca ataatggcct taactggctt
    38341 tacttcgtta ataagacctg cagccggacc catagcaaat acgcagtgct gtgtatcgcc
    38401 agacacatag cattctttgc taagagcacc ggagataact ggtagcagtt ccttcagacc
    38461 tgcacctgct ttttccattt ccagtgcctt tgcagactgc tcatttttca cataacgggc
    38521 catattttta atacttctct ggcaagtcat ggtatcgatc tctttagaat taacgatcag
    38581 atccttgaag ttctggtgga tggtacactc agtagacgcc acaaaacgtg ttcccataac
    38641 tacgccttct gcgcctaatg ccagagccgc taacagtcct ttcccatctg ccacgccgcc
    38701 tgctgccagc accgggatag atacggattc tgctacgcgg ttgatcagga taaagcttcc
    38761 cagttcatct aaacctggat gtcctgctac ttcatagcct acgatagtaa cagcatcagc
    38821 tccaaagctt tctgcctttt tcgcatattt tacacctgga cacttgtgga tcagtttaat
    38881 gcccgcttct ttgatgtgag gaacaaattc agaaggattc tgtcctgctg tttcaataac
    38941 ctttacgcct tcttctccgc acacatcaat atactggtgg attttttctc ccatattcag
    39001 gccaggagcc agtgaaatgt tgactgcaaa cggcttatct gtcagttctt ttgtcttctt
    39061 tactgcagcg cggaaatcat ccatttccgg gtaaatggtg atattaatgg ttcccagtcc
    39121 gcctgcatta gatacagctg ctgccagctg tggtacacct aagtgctgca taccgccctg
    39181 cataattgga tgttcgatac cgatcaattc tgtaaatctt gttttaaaca taagatatac
    39241 tcctttcaac ttgaatgtcc tctcgaaatc tttcatgcgc agaaaaatct ccagcgaaca
    39301 tatgaccatc agcgcagatc atctgttgtg aatattataa caatgcagtc ggatcatgtc
    39361 taatatccag tttttatctc atgtatagct tacatgttat ggatcgttat tttcaagctt
    39421 cttatccatt ccttttcggg caattcatct tttgtctcta ttttttgatc actaattcta
    39481 ctgggcagtg gtcggaacca aacacttctg tatggatctt tgcgctctcc agctcatctt
    39541 ttaagctttc gctgacacag aaatagtcaa tacgccagcc tgcgttcttc tctcttgctt
    39601 taaaacggta ggaccaccag gagtaaattc cttctgtatc cggatagaag tagcgccagg
    39661 tatcaataaa accggcattt aaaaggtccg tgaacttgcc tctttcttca tcagtgaagc
    39721 ccgcattctt ccggttagtc tttggatttt tcaggtcgat ctctttgtgg gctacgttta
    39781 aatcaccgca gaagatcacc ggctttttct cttccagctt tttaagatat gctaaaaatg
    39841 catcttccca ggtcatacgg taatccagtc tggcaagacc atctttggag tttggagtat
    39901 agcaggtgac aatgtaatac tctgggaatt cagctgtgat gactctgcct tcatggtcat
    39961 gttcctcaat gccaaggcca taggaaacag agataggctc ttcttttgtg aacatggctg
    40021 tgccggaata gccttttttc tcagcatagt tccagtactg gtgatagccc ggcagatcca
    40081 gatcgatctg gccttcctgc agcttacttt cctgtataca gaatacatct gcatctacct
    40141 cattgaaaaa gtccataaag ccttttccca cgcaggcacg cagaccgttt acattccagg
    40201 atatgagttt tttcatgatt gtttctcctt ttgctgtatg gcaacgcccc cgccttatgg
    40261 cggggaaagc agttgtgctg ataacacatc cccgcataaa gcgggaaaac tgttgcactc
    40321 cactgccccg cgaagcgagg caaaatactc tcccttattc tggataaacc agtctggact
    40381 ccgggaactc atcaatatgg gtcagcacct tcagatactt tgcagccaga tattttgcca
    40441 ttcccaggtt catatccagg taattgccgc agtcttttgg agatgcgcct ggtacttccc
    40501 cttcaaagtc acggacaaat tcatacattt ctactactaa tggcagaatg tcttttgact
    40561 cataatcacc tgctaaaagc agataaaaac cggttctgca gcccatcggt ccaaagtata
    40621 cggtcttgtc tccaaaatcc ttgtggtttc ttaaaaaggt agcacccaga tgctcgatgg
    40681 tgtgcatttc tgcggtgttc ataacaggtt cttcgtttgg ggaagtcata cgcagatcaa
    40741 aggtggtaac ggtgctgtct cctactttgt cttttctgga tacgtaaact cccggctgta
    40801 atttaatatg gtcgatggta aaacttgcga tcttttccat ggttatttcc tcttttctct
    40861 ctttttcact tatatttccg ctactgcata ccttccccgc gctgcgggga aaacacccct
    40921 agaaatggca gaaaggcagg tcccagcact tcccggttcc tgccctcctt actcttatcg
    40981 ttctttatac tacgaccgcc ccaaaggggc gaccgttttt gatcctattt tttatccgaa
    41041 tgattagtca tcctcttcat cactttcagt tacagtgaag gtaaatctct tacgtgccat
    41101 ttcatcgctt tccagatact catcgtaggt agtgatctta tcaatcagct tgccgttgat
    41161 gatctccata atacggttgg cagtagtctg tacgatctgg tggtcacggg atgagaagat
    41221 catcacgcct gggaacttga tcataccacg gtttaatgcg tcaatagact ccatatctag
    41281 gtggtcagtc ggctcatcta acatcagtac atttgcgccg gagatcatca gcttggataa
    41341 catacaacgt actttctcac caccggataa taccttaacc ttcttaacac cgtcttcgcc
    41401 tgcaaacagc atacgtccta agaaaccacg tacataagtt acgtccttct caggagaata
    41461 ctgggtcagc cactcaacaa tggtgtcatc atttgcaaac tcagcggagt tatccttcgg
    41521 gaagtaagac tgggtggttg taagtcccca cttgtagctt ccttcatctg gctccatttc
    41581 acctgcaagg atcttgaata atacagtctt tgcacgctcg tttggtccaa ctaaagcaac
    41641 tttgtcctct cttccaagga taaaggagat atcgtctaag atcttctcac catcaatagt
    41701 cttgcttaag tgttctacag taagaacttc atttccgatc tcacgggccg gacggaagtc
    41761 gatatatgga tactttctgc tggatggctt gatgtcatct aactggatct tctctaaggc
    41821 tctcttacgg gaagtagcct gcttggactt agaagcattt gcagagaaac gctggataaa
    41881 ttcctgtaat tccttgatct tctcttcctt cttgcggttt gcttccttca tctgcttgat
    41941 gatcagctgg ctggactcaa cccagaagtc gtagttacca gcgtacagct ggatcttgcc
    42001 gtagtcgata tcagcgatct gtgtgcagac tttatttaag aaataacggt catgggatac
    42061 tacgataacg gtattctcaa agttgatcag gaactcctct aaccatgcga tggcatccag
    42121 atccaggtgg ttggtaggct cgtcaagaag caggatgtca ggatttccaa acagtgcctg
    42181 tgccagtaaa actttgacct tctgtgcacc taacagagtg ctcatctgtg catagtggaa
    42241 ttcagtacca atgccaagac catttaacag gttctctgcg tcagactcag cttcccagcc
    42301 gttcatggta gcgaactcac cttccagttc tgctgccttg ataccatctt catctgtgaa
    42361 gtcttccttc atgtagatgg cttccttttc cttcatgatc tggtacagtc ttgcatttcc
    42421 catgataaca gtatccagaa cggtgtactc atcatatttg aaatggtcct gctgtaagaa
    42481 ggaaagtctc tgtcccggag tgatggcaac ctcaccgctg gttggctcta actgtccgga
    42541 aaggatcttt aaaaatgtag actttccggc gccgtttgca ccgatcagtc cataacagtt
    42601 gccttctgta aacttgatat ttacatcttc aaacaatgct ttcttgccta cacggaggga
    42661 aatattattt gcacttatca tgtcatttac ctttcttata aataaatctg ttgtttttgt
    42721 ttccgctaaa agagggggca tttgctgtcc cctcttttct gtcttatgta cttacaggat
    42781 gacctgaaaa atacattctt cgctcaatat cagattgtac agggaaaaac cgcattttgc
    42841 aagggctttt tgcaggaaat cccctgtttt ccccatttaa ccgctgatgc ccgtaatacg
    42901 ttcatccgct gccagtttta acagcattgc attcttatca tataaaggcg gatatactac
    42961 ttccagctct atctttgtgc cgttctttcc ttttcccacc ttcagatttg aaatcttgac
    43021 cttttcttct gaaaaatgca tctggatatc cgcgatcaca ttttcataat ccgtacaggt
    43081 cattttaaga aagccgcgga tcggctccgc agtaagaaaa gaagttctgt gaaggagctc
    43141 ctggatcacc actaccagta taccggcgca gacaccaata ccatactttc ctgctcccac
    43201 tgccattccc acgccagctg tggtccaaag tcctgctgct gtagtcagac cgcttactgt
    43261 attatttcgc acaaagatca cacctgcacc caagaaacct acaccagata cgatctgtgc
    43321 cgccacacgg gaggaatcta ccttttctac atcgtaaaaa ccatacttgg atactaccat
    43381 cataagcgcc gcccccaacg ctacaatggc atgtgtcctc ataccggcgc ttttatccct
    43441 gttctttcgc tcatagccga tcatataccc caatacgctg gctacgatca ctcgcaaaaa
    43501 taactccgca tctgacggta tccctatttt tgttatcatt tctgctacca tcttcatgtc
    43561 cctcctgatc ctgtctctta tactgtgtag cttttatatg ctgatctttt cttctctctt
    43621 aataccagta tagcccatcc atatccacct ggttttgctc atttttcagg catctgtgct
    43681 cattttccaa atgatttcct gtattccacc ggcgtgcagc cttctctttc tataaacagc
    43741 tttgtaaaat aactctgact gtaccagcca cattctacgg taatatcctt taccgtcata
    43801 tgggtggttg ccagaagctc ttttgcatgg tttaagcgaa gcccagccac gtaatcactg
    43861 tagcgcacgc ccagcgctgt ttttaaaaga ctgctgatat aactggcaga cagaccgaac
    43921 cgctcggaaa gatctgccgc tgaaatatct tcccggtaat gttcctcaat aaaacggatc
    43981 atggaattga tcctggagtc ctcagaagga ctgcttaaaa ggcttctgga tttctgctcc
    44041 aggaagtttt tcagttcttt tggatccatg ttttctgaaa ccggagtttt taagaccaga
    44101 ctgaaaaaac tggcaatatt ttttcttgac cgcccgatcc attccttttc ctccatcttt
    44161 ttcccgatcc aggaagataa actggaaaag ctgttgtaat ctccgctctt ccatgcctcc
    44221 tgccacttta agcatctgcg gccgaactcc actttttctt cctcttcatc taaaagagga
    44281 ctgagataat aaaaacgccc ggttcctttt attacccgca ggcaactgag ttcttccaga
    44341 tttccaaggt cttccctcag gttttccatg tcccttgtaa cagaggtgcc aaaacaggtc
    44401 attccttctg caaatgtccg ggcagataaa gtcctgtaga tctcctgtat cccttctcct
    44461 ttttccgaaa aacagagaag tttcaaactg ttatcactga atacagaaac tacatgatcc
    44521 tcatatccct gaaacagagc cgggatcgga tcggaaacca ggttttctcc atccactgtg
    44581 atcatcagca gcccgcagaa ataaccctta tattccccca gtccgatatt tcttccattc
    44641 aaagcacttt ccagccaggt acggaacaga ttttttctcc gctctcctgc cagatactgc
    44701 tgcaggcgta ccgccgtaat ggtctcctgg atcgtctttt tatccaaagg cttcatcaga
    44761 taatcttcca ctttcagctt taccgcctgc ttggcatact caaattcatt gaagccggtc
    44821 atgatataat aacgggtatc cggagatatt tccttcccca gttttatggc ttccagtccg
    44881 gaaggccccg gcatcttaat atctacatat gccatggcga tctcatgttc ttccagcatt
    44941 tttaacatct catggccatt ataggcatga aggacctcgt cttctttgga acatgcctgg
    45001 atcagttttt ctatggaaat atggatcagc ggttcatcgt cgatcaccag tatcttcatg
    45061 tttttcctcc ttgaaaggaa cgatcagtac ctgaatggtt cgtccatcta tacggtaaat
    45121 ataaagccgg atatcaggtc cccacaggct gatcctgttt tctacatttt ttattccaat
    45181 ggactccggg gagtttaatt cttcctcttt aaatcccaca ccatcatcct caataagaat
    45241 gtacagactg tttccgtgac gttctgcctc tacaaacaat ttacatggaa cttcgctggg
    45301 ctctatgcca tggatcactg cattttccac cagaggctgc aataacagct ttggcatgga
    45361 aagggactta aactcttccg aacagttgat ctcataggaa agccggtttc caaaacgcag
    45421 caggcacagg gaacagtaat catttaaaaa gtccagttct gcccctaaag tagtctcccg
    45481 gctgcggctt aacacatacc gcagcagatg ggcaaaggag taaaaagcat catttaaagc
    45541 ctcctgctcc ccgatctggt ttaaggtaat aaagctgtaa atggtattga tgataaaatg
    45601 aggctggatc tgggactgga gcgcctgtaa ctgcaggttt ttctgggcca gcttcagctc
    45661 atattcattg cggatggtct cctggagctg ctttgccatg gcattaatag aagttccgat
    45721 ctcattaaac tcacggatca ccaatggctc acaccgtgaa tttaagttgc ccacctggag
    45781 ttctccaagg actgccttaa tatgataggt agggcgctcg atccagtaat tatttttctt
    45841 aaacatggca tatcccagga tggagcataa cagacagcag gctgctgcca taaacaggaa
    45901 aatggccata cggtctatca cataggacgc agacagcagt acatggaccg ttagaggata
    45961 atgctctaag gtctgtgaaa aacacagcca gtttctgtgg tcatagttta ctttctgctg
    46021 ctttaaacct gcagtctgag tctttgtaag aggcttattg gaatagatca gctgatcttc
    46081 gtcatttgta aacgcaatga tcagaggcgc cttgctggta aggctggaga acagattttc
    46141 aaaatcatct gtttttaagt tcagcatgat cacattttcc tgatcctgaa ggaacatgtt
    46201 gtttaatttt ctggatatgt aaaaacttcc tgtatccagt tcttcttctt catctgcccc
    46261 tgtcatatct ttttccggga taatagccac ccggccgttt ttgtcaagag ccgactgata
    46321 ccagctgcgg cctgtaagag ggtggatggt tggaagatac tgataccagt cagacgtgtt
    46381 aaaatacagc acatttccgt ctgacatgac taacatatcc ccaaaatctt ctctggaata
    46441 ggtagtcagc tgtaaaagtt ttccaatttc ttctcctacc ttgttctggt catagggtgt
    46501 caggtcctca aaaggcgtcc tggactgaaa aaaggaatga taataaggtg cttttgtcag
    46561 gttatcaata tcgttaagat attttgtcat gtatgccgag atcagtttcg tattattctc
    46621 cgcccggctg cgtatctcct gatactggta agttccaaac agataggaca ccagaaagct
    46681 gaccactaca attggaagga gacagctgag gataaaattt acaaataatt ttctggaaag
    46741 ggactgtact ttcttttgtt tcatatgtaa cctcttcgtc tgattaatat tgaattatag
    46801 cacaatttta ttgctggtaa aagtttaatt tttgtcaatc tttacaaaat ttttacttga
    46861 ctggaatttt tcttatatta taaattcccc agtatacaaa acacccggca aacctgttgg
    46921 atctgccggg tgtatgtctg cttcgcagcc ccttgttttt gactgcgcga cttacttatt
    46981 tgaataattt agctacacgc tcttccatgc tggaaactgc ctgctctggg gtgtagttgt
    47041 cttccaggat gcacttctgg atctcgtttg cgaagatttc ttttgcttcc tgatactgtg
    47101 ggataaatgg tgtcttatag gtgctgtact gtacctgatc tacgatgatc tgaagatttg
    47161 gatcttcttc taccatcttc ttgtattctt cagagtctat gcagctctgt ctggtaggaa
    47221 gatatccggt accatttgcc cagcgtactg cattttcggt gttggtcatg tactttaaga
    47281 actcccagcc agccttctct tcttcttcgc tgtggccctt gaacattaac aggttagaac
    47341 cacctgcagg ataagaaagt gtggtatcct gaggaactgc acctacacca acttcaaatt
    47401 cacaggtctt ctctaatccc tttaactgag cactgctctg ctggatcata gctgctgtgc
    47461 cgcctgcaaa tgcatttctg caagcctcag aagagttgta atcctgaccg gatggaacat
    47521 gcatggttcc gtctgcgatc atttctttcc acagatacag tggctttgtt ccaggatcgc
    47581 ctgcaaatcc gatgccgtct tctgtctcat tgaaaatggt tccgcctgcg ttcataacca
    47641 gtgacatgta ataccactga tccagtgggc aggaatatcc ataagaaccg tttccagtaa
    47701 gagccttagc atctgccttt aagtcatccc aggttacagg agcttcttcc agacctgctt
    47761 ctttgaaacg atccttgttg tagtacagta ccggaacgct gcgggaatga ggaagggcta
    47821 ctaatgctcc atcatagtag gaagcgtcta acagacctgg catgaaatcg ctcatatcgt
    47881 agttctcttc atctacgaac ttctgaaggt caactaactg atctgcgtca gcgaaaattc
    47941 ctaacatacc aatctccatc tgagctaaaa caggagcttc gccagctgct aaagcggcct
    48001 gcatcttagt acctgcgtct gtgtaagaac cctgatgtac tgcttttaca aagatcttgt
    48061 cctgggactc gttaaaggag tctgtgatct gctggatcag ttctccgttg gaagaaccca
    48121 tgcagtgcca gaactcgata gtgatcttgt caccgttatc tgctgcagct gcttctgtgc
    48181 ttccctctgc tgccttggtg tcctctgcct ttgctgctgt agtatcagcc ggttttgcgg
    48241 aactgccgca tccagctaac ataccagctg ccatagctgc tgccaatact cctgctactt
    48301 ttcttttcat tttctttcct cctgtgtttg tttaatatac tttttattta taatttactt
    48361 aataccggaa tatacaaagg aattacggat cttctggctt gccagcatgt atacgatcag
    48421 gatcggcagt actaaaacta cgtttcctgc catgataatg tgccacatct gtaaagtctc
    48481 agaagattta agggctgcga tacctacagg cagtgtacga agtgtctcac tggaggtcat
    48541 gatcaacggc cagaagtagt tgttccaggt agagatgaag gacagaaggc cgatggtgat
    48601 cagagccggt tttgccatag gcatcattac cttgaacatg atcttacggt tatctgcatc
    48661 gtccagtctt gcagcctcaa tgacttcctg agggatctgc ataaaatact ggcggagcat
    48721 gaaaatacca aatggattgg aaataaatgg taatattaac ggtataaatg tttttaacat
    48781 cttcatccgg ctgaacatca ggtaaaccgg aaggaaggtg atctgctgcg ggatcattaa
    48841 tcctaacagt acaattccaa aaaagatatt ttttccgaaa tattcatgtc ttgcaaagct
    48901 gtaagcagcc ggtacaatga tcagatactg gagaaccagc acacaaaggc tgactacaac
    48961 agagtttcta aaatataatc caatgggaac gatctcaaat acatgagcgt agttttccca
    49021 gtgaagactg gatgggaata gggtaggcgg gaactgcagc gcctcggtat agtcctttaa
    49081 tgacgtgctg accatccagt aaaatgggaa tgctatgatg accataacag ccacttttaa
    49141 aataatgttc aggatatggc atactttctg aaaaagaagt gcgcccctgc tttcttccat
    49201 gatttttcct ccttaattat atcgtaaact gtggaaattt tactgataat gtacacgttt
    49261 ctttcctaca ccgaactgaa cgtaggtcat gatgcatacc aggatcagta agatcacacc
    49321 tgccgcacag gccatacccg gttttccgta gcggaaagca aaggaataaa tgtagtaaac
    49381 cagtgtattg gtagagtcta ccggtccgcc ctgtgtcata acgttaacgg agtcaaagat
    49441 cttgaaagat gcaatggatg ccacaacaga ggtaaacagg atggtcggtg agatcattgg
    49501 aagagtgatc ttgaaaaaca ctctccactt tggagtggaa tccagttctg ctgcctcgta
    49561 aatattagaa ggaaccgtct gaagggctgc catgatcagc agtacatagt aaccaacgct
    49621 tttccatacc attaccagaa ccagtgaaaa catggaagtg gatgggctgg aaaggaatgg
    49681 gaattttgca aatcccagct ttgtgatgac catgtttaaa aatccggtat ccgggtccat
    49741 cagccataag aatacaatgg atacggaaac catggaaata acatgagggg taaagataaa
    49801 gccctctacc atgttgttta atttcttatg ttttgaaccg tttaaccata ctgcaatgat
    49861 cactgccagc accatggtca ggctgacgaa cagtactgta taaacaacgg tatttacaag
    49921 taccagccgg aatgtttctg aaccaaacag ttctttgtag ttgtccagtc ccacgaaggt
    49981 tttcttcgcg gagatcaggc ttccggaaaa catggaatag tagatcagat aaaataccgg
    50041 atatacagtg aagacaaaca gtaagatcat tgccggcata gcggtaacaa atcccagcat
    50101 gcggtcccgc ttttcgctga cgcttcgttt tttcttatcc actgcttaca cctcccggcc
    50161 tgcaagtttt gcaaatccag catggatcat cagtctgcgt tcgtcatcta aggtgatccg
    50221 ctttccatcc ttatcaaaca gatacatgtt ctttacagga atagacagtt ttaaggtgct
    50281 ttcgtcttct gcttcataat cagattttgc catcagcttt cctgccggac tgtctaagga
    50341 gtagatgatc tcagaaccaa gcatttcctt ggtagcgatc ttagcactta tctctacatg
    50401 ctcttcttct acttctttta agtatacttt ctccggacgg aaaccaacct gcattccttc
    50461 cggcagaatg tcttttacaa tgttcatctg aggagtgcct ataaactgtg ctgcatatac
    50521 gcagtttgga ttgttataca gttctcttgg gctggactgc tgtacaatgt agccatcttt
    50581 catcagtaca atatcatctg ccatagacat tgcttctacc tgatcatggg ttacatatac
    50641 gaaagtggtt cccagttttt tatgtaaacc gatcagctcc acacgcatct gtccacgcag
    50701 ctttgcatcc aggtttgaca gtggctcgtc cattaagaat acttttggct gctttaccat
    50761 ggctcttgca agtgcaactc tctgtctctg gccaccggaa agtgtagccg gtttacggtc
    50821 caggtactga gtcaggccta ctacctcgca gatctcttgg actcttttct ttctctcttc
    50881 ttttggcact ttcttgtttt ccagaccaaa ttcgatattt tcacggactg tcatggtcgg
    50941 atacagagca tagttctgga aaaccatggc aatgtctctt tttcctggtg ttaagtcgtt
    51001 gatcttcttt ccgtcaatgt aaatatctcc ggaagtcggt tcatcaagtc ctgtgatcat
    51061 acgcagtgtt gtggattttc cacagccgga cggtcctacc agaacagtaa agctgccgtc
    51121 ttctatgctt aagttcaaat catcaatgac catctggtca ccaaacttct ttgttacatt
    51181 tttcaactga atagccccca tgccatttcc tcctgttttt cttcgtttta tccttggttt
    51241 gatcttgttt tcagttattt tcgttatttg aacatttttc atgttccttt gttgtatcca
    51301 atataacatc tttgcttatt tttcttcttt gctctttttt tagaaagctt tgctgttttt
    51361 atgaacagag caaaaaataa acttaaagga atcttaattg aattttgggg gcgggaatgt
    51421 taccatggaa ttaacctgaa tattactcca tttactgagc ttacagatat ttatagatct
    51481 ttatagaaaa gaggaatcat cattatgaaa aaaagacatg tgtatgacct tgtaatactc
    51541 cttgcagccg gtacgattat gatgtccgcc tgcggcaaaa aaccagatgc gcctgctgat
    51601 acaacagcgc ccacacagac cgaaagccag actgaggctt ccgcagagga aaccaccaaa
    51661 ccagacgaaa ctccatccga aacacaggtc ccggaagata cagaaagcac aaaattctat
    51721 atgctccagg gaacggttac aaaagcagaa aaagacggct ctgcctttac cttactggcc
    51781 gatgacggaa atgaatatac catcgctctt tctgatatcc gcgatgtgga aacagagatt
    51841 gaaaagaata tccagattgc cattgcctgt atgaaagacg cctccggcga tcctgaaaaa
    51901 gcagcttata tcgtcgcttt cccggaacag gaagaatgga ccatttccac tgacgaaggc
    51961 atcaccacca gcaacgccat gagcaccttc atgatccaga caagtgatgg caaagagatc
    52021 ggatttatta aagataactg tccttccgat gaaaacgcgc ttaccagtga cagcggagac
    52081 tctgtgatcg ttacttatgt tacttctgat aatgcaaact tcccgttgga gatccggaag
    52141 gggaagtgat aaaatcaaaa atctttatat aaaataattt tagctgccag atgactattg
    52201 ctttacagtc accggcagct tattaaaata atctatataa ttgtctcttt tataactctt
    52261 ttttcgtact ttaacttatt ttaagttata atgcaatatt aaagttaaga tctcattccc
    52321 tacagcagat ttccatatct tcccaggaaa cttctggttc tttcattgtc agaagcaaat
    52381 accttttccg gtgctccttc ttctacgatc acaccacctg ccataaagat gacgcggtgg
    52441 gaaatatctc ttgcaaaggc catttcatgg gttacgatga ccatggctat gtgcagatct
    52501 gccagggact tgataacctt taatacttct cccgtcagct ccggatccag agcagatgtg
    52561 ggctcatcaa agaacaggat ctttggtttt aacgccagag ctcttgcaat ggctactcgc
    52621 tggcactgtc cgccggataa ctggcaggga taggcatttt ctttatcttc cagacccatt
    52681 tttttcagaa gttcccttgc ttctttgtaa acttcctctt tgtccctttt ctgtacatgg
    52741 atcggggcat ctgtaatgtt ctttaataca gaaaaatggg gaaacaggtt aaagttctgg
    52801 aataccaggc catactggct ctggatctct tttaaatctt tcttgtctgc atagtttaac
    52861 tttccgtttt catcgctcca taccgctttt ttacccagat aaacgatctc tccgctatcc
    52921 atagtctcca acatggtagc gcaacgcagc agagtggatt ttccggaacc ggaagggccg
    52981 atgatggaaa cgatctctcc atcttccaca cttaatgata tatccttaag aacattcagg
    53041 tcatcaaagg acttttccac atgattgatc tctaataatt tcattatcca caatcctcca
    53101 gtttttagta cacactaacg gtaataatcc agttttttct ccagccactc aaggaacatg
    53161 gctactacca ggttaaatac ataatagaaa ataccggctg caacaaaagg catcatgctt
    53221 acctgggaag aagccagtgc ttttgctgtt gtgaacattt ccataacact taaagtaaat
    53281 gccagggaag tatcctttac cagtgtgatc acctcattgg ttacagaagg aaggatcctt
    53341 ttgatcacct gaggaaggat gatcttgata aatgtctggg acttgctgta tcctaacagc
    53401 tttgctgcct catactgacc tacaggcata gactggatac cactgcgata gatctctgca
    53461 aaataagctg cgtagttgat cacaaatccg ataaatacag cccagaggcg atagctgttg
    53521 cctacacgca taccgaaaat atagtaaggt ccaaaataaa ctaccagcag ctgcagcatc
    53581 agcggagtac ctcgcatgat ggagcaaatt gaacaggtac acctaatttt tcagaaacag
    53641 catcgacaat ctgactcaat gaatatttag cattt
//
LOCUS       DBMDUA010000002        49549 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_656_length_49549_cov_4.110662, whole genome shotgun sequence.
ACCESSION   DBMDUA010000002 DBMDUA010000000
VERSION     DBMDUA010000002.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 49549)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 49549)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49549
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_656_length_49549_cov_4.110662"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            104..535
                     /locus_tag="ACZPCL_00230"
     CDS             104..535
                     /locus_tag="ACZPCL_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR1160955.1"
                     /translation="MSECFYCEDGEKRKSLMIEICKLNYSTIYLNRNQKYPGRCVVKL
                     NEHKTEYFQMTKEQRDGYFEELAATAKAIFELYHPDKINYATFGDLVPHVHVHVVPKY
                     KDGPDWGNPFDDTQGKKELTDAEYQKMVDELKETILKELNK"
     gene            555..1646
                     /locus_tag="ACZPCL_00235"
     CDS             555..1646
                     /locus_tag="ACZPCL_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015445.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR1160956.1"
                     /translation="MKKMLAAALAASMVMGLTACGGSSQPTETKAPENAKETEAAAEG
                     TTEAAAAGDEEKKTGSEMTWKLATDAAKDYPTTKALIKFADEVYEKSNGRIAVDVYES
                     SILGDEVSYMEQLQAGTVDVAKLSIGTLSGLYEDTQVFLLPFLFKNNTEMWKVLEGDV
                     GTTIKNGLNDYSIQGIGFTDCGSRCFYTTTEVKTIDDFKGLPIRVQNNQLMNSMVSNL
                     GANPVNVAANEVYSALQTGVCKGGENNPNIILSDSLYEVAPYVLMDNHTTTMDVICMN
                     LDLWESLSDEDKAIVDEAMADATAYDREIWDASIDESLQTLKDKGATIVEPDDATLDS
                     FREAMAPIYEEYSAKYATLLDAINTTLGK"
     gene            1683..2195
                     /locus_tag="ACZPCL_00240"
     CDS             1683..2195
                     /locus_tag="ACZPCL_00240"
                     /inference="COORDINATES: protein motif:HMM:NF016200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR1160957.1"
                     /translation="MKEKIENAIGLVYTVVEALCRLVLIFMVCTVTAQVISRALGGNI
                     KWCEEVMLILLDTMMFLLLPVGIKDDLHIRIEVFAKYFPRKVKVFLLYFSNAILLVVS
                     LCMIRYGFFLMEKTNSKFTITGLPRRYLYLVTVISGILCLVMVVAKFFGMFHTKSTDN
                     FLESKSELEN"
     gene            2234..3532
                     /locus_tag="ACZPCL_00245"
     CDS             2234..3532
                     /locus_tag="ACZPCL_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR1160958.1"
                     /translation="MDTNTIAMIILFATFMIFMVLRVPVTFSMLGACVLTALYLGPKY
                     VSSVFLRLGDGVQSFSFLAIPFFIFAGDIMAEGGISDRLVEVANVMVGRFRGGLAYVN
                     VIASTFFGGISGSPTADVSSLGPIEIGMMTKSGYDKDFSVATTVATATQALIIPPSHN
                     MIIYGMAAGGLSTGKLFLAGIGPGLMLCAAMCILVARITRKRGYPRGPKYTVRESLKI
                     VSDGFLGVMTIVIILLGVCTGLFTTTESAAIACVYALFVTLFIYKSIKVKDIWRIAKK
                     SLGTLAMILSIIAAANAFCYLMSLMQVPAKITQMLLTVSDNKYVVLILINILLLVLGC
                     FMDVAPLILIVTPILLPVVKALGMDPIHFGIVIMMNLAVGSLTPPVGTTLFAGCAVGD
                     IPIEKATKALLPYYAVMVVALLLVTYIPAFSMTLPNLLMH"
     gene            3551..5209
                     /gene="ilvD"
                     /locus_tag="ACZPCL_00250"
     CDS             3551..5209
                     /gene="ilvD"
                     /locus_tag="ACZPCL_00250"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR1160959.1"
                     /translation="MIREWNEESLDHRNALLYAMGISEEDAKRPVIGLVNSWNEMNPG
                     HFPFDKTVIQEMKDEIYKAGGLALELPVTGICDGICSNTPGDRYTLPARDLVSSEVEM
                     VAELNMLEGMVIMATCDKVVPGMLMGAFRVNIPTTMLTGGYMAAGCYEDRMLTLTHTK
                     QAYAAYVEGDMSREEYKAIVRHACPTPGACPFMGTANTMCAMAEILGFSPHGNASVRS
                     QSEKWHQMAREAARKVVEAVKEEKRPSDFVTQKSLENVVRYMMATGGSTNSLLHIPAL
                     ARQMGFDITPETFDAISREVPLISTIYPNHPVYTMEEFDRAGGLGAVVKEMVKAGKID
                     ADADGMFGTIRQKAELAENMDTDVIHPVAEPISEQGGLAVLHGNIGTDSAIVKFSAVA
                     ESAWIFDGPAKCYDSQDDAWHAILKDEIEAGDVVVIRYEGPKGSPGMPHMETFMAAVL
                     GKGLGEKLALVSDGRFSGATGGLAIGHVSPEAYEGGNLALIQDGDMIHIDIRARKLTV
                     DVSEEEFERRRKDWKPVEKPAMGWLKLYKNNCTSAHRGATIYWD"
     gene            complement(5312..5890)
                     /locus_tag="ACZPCL_00255"
     CDS             complement(5312..5890)
                     /locus_tag="ACZPCL_00255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1160960.1"
                     /translation="MTNPKATLHMANGADIVIELLPEAAPNTVNSFIYAASHGYLDHH
                     AIERIVPGNWVDVSYTAFGHKELQYLIPNEFALNPDVTPLDSHPGCVCMGGYGEAGLA
                     SCEFFFPLRDCPDHKGTYPVFGKVISGMNEIRRIAAVKTVPVDFPIPGVEVNKPVEPQ
                     VIETVELELYGETYPEPVRVENPKLPKCWPKL"
     gene            complement(5919..6995)
                     /locus_tag="ACZPCL_00260"
     CDS             complement(5919..6995)
                     /locus_tag="ACZPCL_00260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009533702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR1160961.1"
                     /translation="MNEARANRILAALKEQGVDQMLITDPMSICYLTGISMAPIERFY
                     ALLLKADGHHYYFLNHLFNVPGDVGVEKVWYSDTDPVPEIVAARLDKNAVLGVDKDLK
                     ARFLLPLMEMKAAAGFVNGSLAVDITRGVKDTEEQKKMRISSAINDKAMAKFKGLIHE
                     GVSEKEVADQMLQIYLDLGADGYSFEPLVAFGANAADPHHSPDDTVVKPGDCVLFDVG
                     CIKDGYCSDMTRTFYFKTVSDAHREVYETTKASNETAISKIRPGVPLYQLDAAARDLI
                     SAKGWGPNFNHRLGHFIGLSEHEFGDVSSANTWEAKPGMIFSIEPGIYLTGDTGVRVE
                     DLVLVTEDGVEVLNHYPKELQIIE"
     gene            complement(7146..7982)
                     /locus_tag="ACZPCL_00265"
     CDS             complement(7146..7982)
                     /locus_tag="ACZPCL_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1160962.1"
                     /translation="MIHSLKKLLKSNYLFTLGILICLGWILAAVLAPVVAPYDPIAQD
                     LSLRLKAPSAIHWFGTDNFGRDIFSRVIYGGRYSLLAGCLSVIIAGIIGTFYGALAGY
                     VGGWLDNVMMRFSEMILSFPSLILAMIINAVMGSNLFNTMFALVIVSWPTYARLMRSV
                     VLSVKENEYVNAAEALGASRMRILMKEIIPNSVSSVLIMATTDIGNKILMFSTLSFLG
                     LGSAPPTPEWGMMVSDGVDYFNKFWVAGFPGLAIFTMAVGANFIGDGLRDLLDPKLRT
                     QF"
     gene            complement(7985..9004)
                     /locus_tag="ACZPCL_00270"
     CDS             complement(7985..9004)
                     /locus_tag="ACZPCL_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1160963.1"
                     /translation="MGELKFIGKRVVYLAVMLFGVATLVFILTKMIPGDPVVANLSQR
                     ALNDPEIVAAYRAKHGLDRPLIVQYIYYIRDLLHFDLGTSIRTSRPVLDDLARCYPAT
                     LELALFSIVIASVFGILFGIISAIRRNSILDQIVRAISVTGVSIPAFWFALLMLYFFY
                     YKLKIFPGPGRLSNSFSAPASVTGMYVIDSLIEGNPAKAMDAVRHLILPSIVLAAFTM
                     GLITRTTRSNLLDALSTDYVRTAKAKGLNSICLILKHALGNALIPVMTVIGLGLGNLL
                     GGMVLVETIFKWPGVGQFAYESVMAADYPAIIGVALLIALNYMIINTVVDILYGIIDP
                     RVRCN"
     gene            complement(9195..10835)
                     /locus_tag="ACZPCL_00275"
     CDS             complement(9195..10835)
                     /locus_tag="ACZPCL_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1160964.1"
                     /translation="MKNKKLLAFALSMAVAAGCTACDGGQASTDTSAAGSSDSTQAES
                     SATGDNTVIVAMGSGFSTLDPGYVYEKYPQLIVNACYENLFKFYENNGTPQPCLADTY
                     EFSEDGLTLTVKLKDGLTFASGNKITSADVAFSINRTKNLKGNPSFICDTIESIETPD
                     DATVIFHLNQPDSAILSKLTYSSLAVLDSAVVKENGGTDAEDASSTDTAQSFLDNASA
                     GSGMYILTSYTPDEEVVLEKNPNYWGTSTNVDKYILKIQPDANTQMMTLSTGDIDVAL
                     NLTDDTLEELGSAENVEIVNGTTKMIGFVMMNMNEEYGGPVSNPDVQKAIRKALDYSG
                     IRMICGDGTLTPYSIIQDGFMGSKGTRPDDYTNIEEAKQLLADAGYPDGFDVDMTVSD
                     LDMEGILLTDLAQKVKDDLSQIGINVNITTEAWAAGYGDAYRNGTLGFTVMYWGVDYS
                     DPNVQLEFLPGGTVGQRAGWTAEMNPELAAMYQEAMEATDNDARTQVLENIQDAMYED
                     GPFIVIAQAPAHIAHSNRLDGVDIFDSYTIDLTEINVK"
     gene            complement(10924..11898)
                     /locus_tag="ACZPCL_00280"
     CDS             complement(10924..11898)
                     /locus_tag="ACZPCL_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1160965.1"
                     /translation="MADENKSKDVLVHADHVKVYFKGKDKKSGTVRAVDDISFDIFAG
                     ETFGVVGESGCGKSTLGRTLIRLIPATDGHVYLDGEDISGLKGKALKQMRKKAQIIFQ
                     DPSACLNPRRTVRQILMEPFQIHHMTGSMDVDARIEELLHLVGLDTYHLSRYPHELSG
                     GQKQRIGIARALALEPKLIICDEAVSALDVSVQAQVLNLLQELKERLGLTYFFISHNL
                     NVVYQVSDRVGVMYLGKMVEIAAYDQLYEKRYHPYTEALLSAIPQVDADDRTERIHLT
                     GEVPSPSDPPSGCPFHTRCPKACDICSKEIPQLKEIEKGHFVACHLYQ"
     gene            complement(11891..12904)
                     /locus_tag="ACZPCL_00285"
     CDS             complement(11891..12904)
                     /locus_tag="ACZPCL_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1160966.1"
                     /translation="MNQSEILLEIKDLCVEFKTMAGTVHAVDHLSYTLHRGEKLGIVG
                     ESGSGKSVSSLAMMQLIPNPPGKVTGGQILYNGKDLVKLSEREMQKIRGNEISMIFQE
                     PMTSLNPIIRCGKQIAESLRLHRGMKKKEAMEEAIKMMQAVGIANPQARAYEYPHQMS
                     GGMRQRVMIAMALACQPQILIADEPTTALDVTIQAQILDLIRDLNQEMNTSVVFITHD
                     LGVVSELCDTVIVMYNGHIVEKAPTADIFREPLHPYTQGLLSAIPRITKERKPLSTIE
                     GMVPNPVERIKGCRFWPRCPKACDRCRKEEPPVFSVGEDRQVRCWLYAQADNGSQEAV
                     TNG"
     gene            13211..15268
                     /locus_tag="ACZPCL_00290"
     CDS             13211..15268
                     /locus_tag="ACZPCL_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1160967.1"
                     /translation="MKRKTDRGRMKFRAFMVCVLSFLVVTGSACFLNREQEKEEKLKA
                     VYAAESTVSRVKSQLNRYLAESELVKNIIESGYDIDGEKFSILSQMMQDKQNVIEAHE
                     LAKDGIVSHIYPMKGNEEAMGLNMLEHPDRKKEANLAKTSGQYTIAGPFPLVQGGNGA
                     LLFDPVYVKDEAGEDTFWGFSILVLNWDHFMEEVETYKLEDASYQYLIWKNGTELGEK
                     LTIAQSEKFSFKDTLEVACDVPNDTWYFEIEPKAGWVPQSQIAFGILIAALVSGVLTV
                     GYWQYEMQRYKEALYAEKIERAAKRAEEASEAKTRFLFNMSHDIRTPMNAIIGFSDLL
                     EKHLDDKEKVHDYIKKIQLSGSFLLSLINYVLEMARIESGKATLRTEVGDAQELLGAL
                     NAVFEPAVEKKRLKYNCTLDVEHRFIICDVTKVREIVLNIISNSVKYTPEGGSVTVQI
                     KEIPWEKEGWTAYRILVEDTGIGMGAEYLPHIFEEFTRERTSTESKVVGAGLGLPIVK
                     ALIDLMGGTIQVESERGKGSKFEVILPFEIASEEEVKDSYVKKEEKLYNRSKEKRILL
                     AEDNELNAEIAITILEENGFKVERAEDGCKCVELFSEKPAGYYSTILMDIQMPNMDGY
                     TASRKIRGMEREDAKAIPIIALTANAFDEDRNKAFAAGMNGHIAKPIDVGRMVRSIGA
                     LVK"
     gene            complement(15415..16143)
                     /gene="nagB"
                     /locus_tag="ACZPCL_00295"
     CDS             complement(15415..16143)
                     /gene="nagB"
                     /locus_tag="ACZPCL_00295"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIR1160968.1"
                     /translation="MVIYKAKNYQDLSRKAANIISAQIIMKPDCVLGLATGSSPIGTY
                     KQLIEWYNKGDLDFAKVTTINLDEYKGLDPDNDQSYRYFMNTNLFDHVNIDKARTFVP
                     DGLEPDPQKACAAYNEIIRSHGGIDLQLLGLGRNGHIGFNEPGAAFEKETHCVDLTES
                     TIEANKRFFASEDDVPKQAYTMGIKNIMQAKKILLIAGGEEKAEALKNSLYGPITPSV
                     PASILQLHNDVTVIADEAALSLIM"
     gene            16361..16795
                     /locus_tag="ACZPCL_00300"
                     /pseudo
     CDS             16361..16795
                     /locus_tag="ACZPCL_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543534.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
     gene            16931..17479
                     /gene="spoVT"
                     /locus_tag="ACZPCL_00305"
     CDS             16931..17479
                     /gene="spoVT"
                     /locus_tag="ACZPCL_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HIR1160969.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRLREGTPLEIFTDREGEI
                     ILKKYSPMVELAAFAGQYAEAMSQATGLIVCITDRDQVIAVSGGARKELLQKAISPSL
                     EQVIGERQVVQSGKDEKSFVQLVDEELEGITAQVVAPIICEGDAIGAVAIMSREPRAR
                     FGEMEMKLAATACGFLGRQMES"
     gene            complement(17614..18888)
                     /locus_tag="ACZPCL_00310"
     CDS             complement(17614..18888)
                     /locus_tag="ACZPCL_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR1160970.1"
                     /translation="MSAGIILFAAFAVCLIVGVPVSISLGVASLCSVVFGGLSASSTV
                     IAQRIFGGLQSTSIMAIAFFVLAGNLMTKGGISRRIVNFANCLVGNVRGGMSLALVLA
                     CAFFAALSGSAPATVIAIGTMLYNDMVKLGYPGDRTAGLLVVSGGLGPIIPPSIIMVI
                     YCTLTGASVGNMFKQGMLIGIVIMLVLMAEVLFYAKKENWPKQAVKRSPSEIFKIFLD
                     AIPALMTPIIILGGIYSGMLTATESAAIAVVWAAIAGLFIYKELTVKELVPILKDSAK
                     SSAMILFIIAASTAFSWVFTFSGASQALVNAVISMHLNSALFCFVVAIILLIFGTFME
                     GTAIAVLLVPVLWPIAQSMGIDVIHFGMIVCISNVVGTMTPPVAVNIFSASTVSGLKM
                     GDIAKAEIPFLIGYVAVFFCCVFSETFCTFLI"
     gene            complement(18885..19385)
                     /locus_tag="ACZPCL_00315"
     CDS             complement(18885..19385)
                     /locus_tag="ACZPCL_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR1160971.1"
                     /translation="MKGIKKLLSYVTKVEYAIMIVAFVAMVTAYFVAVVNRNFIKASM
                     PWTEEVAMYSMIYMALLGTEVGLRDGTQVAVTAIIEKLHGITKKVVSIVEQIVLEIFT
                     AVMLKAGTALFLKQIQTGQTTPVLKIPMSAMYFSLVLTFGLILLIQTVILVEKIIDLK
                     NKEAEA"
     gene            complement(19779..21650)
                     /locus_tag="ACZPCL_00320"
     CDS             complement(19779..21650)
                     /locus_tag="ACZPCL_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR1160972.1"
                     /translation="MKQRTQKYLHAILALVLSLCLITGCSLFPNSAPKADKNTSQETI
                     AEEATENSSDDSASQPDEAALAAAREAFDQYTENLFKQEASQSLLTLHYTLADPESYG
                     ITDYDRTLGTASAEDTRKDLEEAKQIKADLEAMDTRLLSKDQLLTYTILSSYINTLLS
                     SDGLELYDQPLSTTLGIQSQLPILLSEYTFYRKQDVDDYLSLLSSIDTYYDSLIAFET
                     ERAQADLGMCDTVIDNIIRSCNAYLIDADHSFMAETFSSRLDEMGGLSDQEKADYKAK
                     NKKAIDEHFVPAYERLISGLESLKGTGSNDKGLYYYPEGRKYYEYLVNASTGTSYSDV
                     SALKQAINKQMSKELAAVNQLLSDDPSLGDKLDSSTFTLTEPKDILEDLKAQSQADFP
                     PVNDCNYTIKNVPKTLEGFLSPAFYLTVPIDRPEDNSIYINNQSTDAGSLYTTMAHEG
                     YPGHMYQTNYFNQHNTCNLRNVLDFQGYVEGWGTYAEYYAYTLDHGMEEQVGELLQHN
                     AAFTLALYALLDINIHYEGWDMDQVNDYLTMYFQIDDPSVISSIYYEVVENPSNYLSY
                     YTGYLEFLNMREEAQQRLGANFSLLEFNRFLLDIGPAPFSVIRNYFTQWLIQNGSSN"
     gene            21877..23856
                     /gene="metG"
                     /locus_tag="ACZPCL_00325"
     CDS             21877..23856
                     /gene="metG"
                     /locus_tag="ACZPCL_00325"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR1160973.1"
                     /translation="MCQDCKKKPYYITTAIAYASGKPHIGNTYEIVLADSIARYKRAQ
                     GYDVFFQTGTDEHGQKIEEKAEAAGITPKEFVDKAAGEIKRIWDLMNTSYDKFIRTTD
                     ADHEAQVQKIFKKLYDQGDIYKGAYEGLYCTPCESFWTPSQLVDGKCPDCGREVKPAK
                     EEAYFFRMSKYAPQLIEYINSHPEFIQPVSRKNEMMNNFLLPGLQDLCVSRTTFDWGI
                     PVTFDPKHVTYVWLDALTNYITGVGYDCDGNSTDQFKKYWPADLHLIGKDIIRFHTIY
                     WPIFLMALGVPLPKQVFGHPWLLQGDGKMSKSKGNVLYADTLVDFFGVDAVRYFVLHE
                     MPFENDGVISWELMVERMNSDLANILGNLVNRTISMSNKYFGGVVENKGVAEAVDEDL
                     KAVVLEEVKKVDAKMAQLRVADAITEIFGIFRRCNKYIDETTPWTLAKDEAKKDRLAT
                     VLYNLTEAITIGASLLESFMPETSEKILAQLNTKKRELDEMDTFGLYLSGNKVTDKPE
                     ILFARMDIKEVMAKVEEMRAAAAEAAKPAEEAAEDDGIDLEPKAEITYDDFAKLQFQI
                     GEIVKCEAVPKSKKLLCSQVKIGTKTRQIVSGIKAHYSPEEMVGKKVMVVTNLKPAKL
                     AGVVSEGMILCAEDAEGNLSLMVPEKKMPAGAEIC"
     gene            24351..25058
                     /locus_tag="ACZPCL_00330"
     CDS             24351..25058
                     /locus_tag="ACZPCL_00330"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease/transposase family protein"
                     /protein_id="HIR1160974.1"
                     /translation="MILNKSGQRAIRLDARATDISGRNFATEMQNDTEQDDMRRRARF
                     YQGLIDTPLLKSGRKTRYKHLPPTIITFITQKDIFGKGLAKYTFTEQCEEIRDLHLED
                     GTKKIFLNMTSKKGEPVLVSLLQYMKHTTLENSEILVKDERIAQLDEIVTEIKQSEEW
                     EAVRMSILSIGLERGMKKGVAWGIAKSILDILEETGPVSEKTREMILTETDAERLSAW
                     VKKAARSNSEEDFLSKI"
     gene            complement(25148..25786)
                     /locus_tag="ACZPCL_00335"
     CDS             complement(25148..25786)
                     /locus_tag="ACZPCL_00335"
                     /inference="COORDINATES: protein motif:HMM:NF040019.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczE/YyaS/YitT family protein"
                     /protein_id="HIR1160975.1"
                     /translation="MKFVKRIVIYLAGLFFVALGVAFSVNSNLGVSPVNSLPYVVSRI
                     LGKDLGTCVTVIFIGYILIQILLLRKAFRPLDLAQILCSFAFGYFVDLTETLLGSFCL
                     PGYPGRLVMLAISIFLVAFGICLYLDTDLIVMPMEGLTAAMNKTVFPKKAFHEVKVIL
                     DCTAVVVGIVLCFVFLGELQGIREGTVLCALLVGKVMKPMQKVLVPTVKRFC"
     gene            complement(25920..27605)
                     /locus_tag="ACZPCL_00340"
     CDS             complement(25920..27605)
                     /locus_tag="ACZPCL_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015355.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015711 - organic anion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HIR1160976.1"
                     /translation="MSLPKRIEITEVGPRDGWQNHKAMIIPTEVKAKYVKKMIDAGAD
                     KIEVTSFVNPKAVPQMANAKDLVALVQPYAKEKGCQTIGLALNKRGVDDAVAAGVDYV
                     GFGISVSEEHNKRNSNRKKINFDVCGLSFKHFVLFFAVVFASAYLGFMPTVTIYSNSA
                     GAFTATTFVGSVAFLMAVGGVFFWLGNTIPILNNYLGGACLLPLLGASLMNYLGLVPE
                     PLANGVRMVMKGGFQDMYVAMLLIGSVLVMDRKLILSATARYLPTIIGSQVFALGFCM
                     IGGAITGFGAKEGLFYIGAPCMSGGSAGAITTLPSLYSALSGQDMTGMAGQFLCYASI
                     ANILAVLMAAVGGAVTAKMSGWNGGGRILVSQSAEELKEEKRAGTSADYKKLGSGIFM
                     SLVIYLLGDILGKLPGTSVIAGLAWSIIIAIVIKCTGILSDDIADNCVYSMNFALKAL
                     LPMLVAGIGINSLKITDLTKFFSPGVFAVIFFGVLGAFIGAMIFAKLAGLFPYEAGVT
                     AGLCCCNIGGSGDLAVLTAADRMNLLAFASISTRIGGALMVVWLGFLYPLLMK"
     gene            27962..29158
                     /locus_tag="ACZPCL_00345"
     CDS             27962..29158
                     /locus_tag="ACZPCL_00345"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015418.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR1160977.1"
                     /translation="MRISLEQALDILMDHVTQGRTERKPLEQCLGLVLSQDVFALLDM
                     PPFSRSAQDGYALCSKDSVGVDAENPVKLKVTGKIYAGDYLDIQVRSGEAVRIMTGAM
                     IPAGADCVLRQEDTDEGENVVQIYKEVEPGGSICFKGEEYKKGHILLHAGTKIDAAAL
                     AVASGNGIMELPVYERVRAAVVSSGSEVVEPGTPLTPGKIYNTNTVYMKARLHQLGAQ
                     VMMSQTVGDDLEVMTGALKEAANQAELVITTGGVSVGQKDLTEEALLSIGAKILFHGI
                     AMKPGMPTLAAEKDGVLFIGLSGNPFSAAIPFEMFVREILSLKMGDPELKLRRETLTA
                     VTGFSKNSRRRRFLRGKAVGKEVWLPDQQANGQMRSMVGCNCLIEIPAGSGPVQAGDK
                     VEVLWL"
     regulatory      29267..29363
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            29565..30845
                     /locus_tag="ACZPCL_00350"
     CDS             29565..30845
                     /locus_tag="ACZPCL_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1160978.1"
                     /translation="MENKTFALKGNIVYTKDKNELCINEQSYLVCENGKVRGIYKELP
                     DELKNVPVEDMGDQLIIPGLTDLHVHAPQYSFRGLGMDMELLDWLETNTFPEEAKYKD
                     LEYAKKAYGIFAEQMRKSAATRACIFATIHKEATILLMDLMEESGLSTMIGKVNMDRN
                     SPDYLREPSAKDSEEATREWLKMVADKNYDHTRPILTPRFTPSCTDELMERLKGIQKD
                     FDLPMQSHLSENPGEIAWVKELCPWSEFYGDAYDHFGLFGGECPTIMAHCVYSGEEEI
                     ARMKERGVFIAHCPDSNTNLSSGVAPVRRYLEEGMNIGLGSDVAGGTSENLFAAMAQS
                     IQASKLRWRLLDASLKPLTIEEAFYMATKGGGAFFGKVGSFEPGYEFDAVVCDDSRLE
                     HPQPLTVKQRLERIIYLGDEREVAGKYVEGRKLF"
     gene            31246..32466
                     /locus_tag="ACZPCL_00355"
     CDS             31246..32466
                     /locus_tag="ACZPCL_00355"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR1160979.1"
                     /translation="MKYDFTTILDRKGKDSIAVEPFEADWIKWPGKTKEGFDIIPMWV
                     ADMNFPAVHTIPEAIIERTKHTTYGYFSPREEYFDAIIKWHKTRNGIEGLEPKHIGYE
                     NGVLGGVVSALNVLCSKGDSVLLHSPTYIGFTKSLTNNGYHIVHSPLVKDENNVYRMD
                     FEDMEKKIVENHIHAAIFCSPHNPTGRVWERWEIEKAMEIYKKHDVYVVSDEIWSDLL
                     LNGHKHITTQSVSEDAKQRTVALYAPSKTFNLAGLIGSYHIIYNDWLRDRILKESSLS
                     HYNSMNLLSMYALIGAYKLEGYEWVDELKEVLSGNVNYACDYIEKHFEGVNVSKPEGT
                     YMLFLDCTEWCKKHGKTITELQAAGIEVGVIWQDGVAFHGPCHIRMNLALPLSRVKEA
                     FDRLDKYVFNLQEA"
     gene            32472..33245
                     /locus_tag="ACZPCL_00360"
     CDS             32472..33245
                     /locus_tag="ACZPCL_00360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR1160980.1"
                     /translation="MIFDTHAHYDDEAFDEDREQLLASFKENGIDAVTNVAASLSSCK
                     TTLELARNHDFMYAALGVHPSESAELSDEGLKDIETWCTERQDGKVRAIGEIGLDYYW
                     EEPDKEIQKKWFVAQLDLARRLKLPVIIHSRDAAKDTLDIMKAEHGSDIGGVIHCFSY
                     TKEMAREYLDMGFFLGIGGVLTFKNARKLVETVEYAPLDRLVLETDCPYLSPVPNRGK
                     RNSSLNLTYVADQLAALKGVDRETVEKMTWDNARKLYRL"
     gene            33335..34198
                     /gene="rsmA"
                     /locus_tag="ACZPCL_00365"
     CDS             33335..34198
                     /gene="rsmA"
                     /locus_tag="ACZPCL_00365"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR1160981.1"
                     /translation="MNEKLSDPKKTIEVIQKYQFAFQKRFGQNFLIDAHVLEKIVSAA
                     GITKDDCVLEIGPGIGTMTQYLAESAGQVIAVEIDTNLLPILADTLKDYSNVKVINQD
                     ILKVDINELVKEYNNGRPIKVVANLPYYITTPIIMGLFESNVPIDNITVMVQKEVADR
                     MQVGPGSKDYGALSLAVQYYASPYIVANVPPNCFIPRPNVGSAVIRLTRYQEPPVQVK
                     DPKLMFKLIRASFNQRRKTLQNGLNNSPEISFSKEEITKAIESLGVSPSVRGEALSLE
                     QFAQLANYFVQ"
     gene            34232..35470
                     /locus_tag="ACZPCL_00370"
     CDS             34232..35470
                     /locus_tag="ACZPCL_00370"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: protein motif:HMM:NF017873.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HIR1160982.1"
                     /translation="MSDKKRWIRLAVAAGLGAAAGAWTYQIQKDKKAKNDARLAVVEA
                     AVVRSRDYGKQKAYIIGNSLSSLAAAVWLIKDCHFPGSSIMVYGENEEACGNAGPLVV
                     SRENCEDFLELCGKLPGSGEKVFTFPKTEGGERLGCADLPALWRLLCTEEERLDVLSV
                     EDWFSGTPHIFETDLWQLCQCVFGLKKDSNLAEFRRSLWELKGPFLFLSPEERQELIR
                     LLKQYLRRKGVLLLENTQVTDLELENGNDNGSGTGLAVKKLYVKRRLQEEETDREAFV
                     FEKIDLRSGDVCIMDNGSGSGKLWKKAADLHIALGEPEAFVGEEDKLQPLRPHHFTDR
                     PKTVPTGSRNLGFVGAFSRLEEGCCLTGNYAVASARSAVDELMHAGRRAAGTEEKRQS
                     RIRKWITVYKVVCSLYRADL"
     gene            35584..35982
                     /locus_tag="ACZPCL_00375"
     CDS             35584..35982
                     /locus_tag="ACZPCL_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1160983.1"
                     /translation="MLFIGGISQGRKLLNYARSILCGSCGSTSQCQVFMTYMYFSFFF
                     IPLFKWNKRFYVQMSCCSAIYELEPEIGKAILRGEEPAITSADLHLVQEGKYTRTWQE
                     GSGNPHKKCMRCGFETDEDYNYCPKCGGRI"
     gene            complement(36124..37059)
                     /locus_tag="ACZPCL_00380"
     CDS             complement(36124..37059)
                     /locus_tag="ACZPCL_00380"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009220445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR1160984.1"
                     /translation="MRLSDLKAGSNTKQTLLLPVASHYPIEMTVICGSCPGKTLVVTA
                     GVHGCEYVGIETLNRLKKELDPIDLSGRIILLPLVNPEGFYMGSKQIIPADGQNLNRT
                     FPGDPEGTFSSRLAKLLEETLYPEADFLIDLHGGDVNEALTPLVFFPTTVSDALAAKA
                     SAAAASLSIPYRVASTAKNGLYSWAAQCGIPALLAERGERGLWCEDEVTACKRNIFEL
                     MAHLKIRPFSDTDLHQEEIRQAVYEEAPKNGFWYPAVSHAGQKLKKGTLLGTLKDIYG
                     NEIFRCTAPFDGVVLYYTLSLGVKCKDPLIAFGEI"
     gene            complement(37076..37855)
                     /locus_tag="ACZPCL_00385"
     CDS             complement(37076..37855)
                     /locus_tag="ACZPCL_00385"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1160985.1"
                     /translation="MLEVNNVCVSFRSERQEKIFGTARNQVLFDVSLKVSQGTCLGIL
                     GESGSGKSTLGRVICGLLKPDTGEVKIQDVSVYASRNGRKNLQKRLSVVFQDYTTSAN
                     PRFRVKEIIAEGLAARERNQKIRLNRLEEINRLLELVGLNSSYANRYPHELSGGQLQR
                     VCIARAVACQPEIILFDEAISSLDAHTQIQIMDLLHDLKEKLGLTYIFITHDLTSVTY
                     LCDDVLFLYHGRITEHIPVSRIAETKDDYARKLLASIIEFN"
     gene            complement(37848..38678)
                     /locus_tag="ACZPCL_00390"
     CDS             complement(37848..38678)
                     /locus_tag="ACZPCL_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1160986.1"
                     /translation="MSAVLKVENLNVTLTYRKASKKLVEDVSFEVHPGECLGILGESG
                     SGKSMTVKSILGLLDKNFQVSGSAVFDGHDLLKESKEELRRLRGSRITMVLQNPMTCF
                     DPLYRIGNQMAETFAAHTAWNAQEIRTCCLKILDQMQILNGEEVLEKYPHQLSGGMLQ
                     RIMIGIAMALQPELLIADEPTTAIDAVTQYEILEEFVRIKKNKNTAMLFITHDLGAIS
                     KVADRILVMNSGQIVDSGDFEHIRNHANDPYTRLLIEKRSAVMCHYRDILHGKEISHA
                     "
     gene            complement(38678..39499)
                     /gene="opp1C"
                     /locus_tag="ACZPCL_00395"
     CDS             complement(38678..39499)
                     /gene="opp1C"
                     /locus_tag="ACZPCL_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt ABC transporter permease"
                     /protein_id="HIR1160987.1"
                     /translation="MLHRFLHNKTAVGTITIVLIVAFMGLFAPFIAPHDPYATNILNK
                     FAHFSKEYPLGTDNLGRCILSRMIYGIRPTLGLAVLTMLGTIGLGALMGLLAGYFRGI
                     TEELIMRIVDVMLSFPSQIMVFAVVALLGINVQNVILANVFIKWAWYARMIRTGVMQY
                     RDRNFVQFSRCVGTPERFILFRHLVPSIAADLAVLSSLDVGWAIINISTLSFLGLGVQ
                     APTPEWGAMLSEAKNVLTSNPAQMLVPGIAIVILVSAFNLMGDAIRDVLDPKEVL"
     gene            complement(39499..40440)
                     /gene="opp1B"
                     /locus_tag="ACZPCL_00400"
     CDS             complement(39499..40440)
                     /gene="opp1B"
                     /locus_tag="ACZPCL_00400"
                     /inference="COORDINATES: protein motif:HMM:NF045469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt ABC transporter permease"
                     /protein_id="HIR1160988.1"
                     /translation="MKPYIIKRILSAIPLLLIISFVCFVFINLIPSDPAEVALRVRQV
                     PIITEDAIAEVRSQLGLDKPYLVRYFTWVLDCLHGDFGISYVNPSRTVAGELLRCMPA
                     TLQLAGASLIMVILLSLPIGFLCAVYKDGWFDRIMRSLVFMTTAMPPYWVGLLLMWLI
                     SIKLDLLPTSGNGTIRHLILPAFTVSLSYISTYIRLIRNNMLENMKQDYVLYANVRGL
                     PQRSILVKHILKNSMHTCIVAIGMSIPQLISGTIVVENVFAWPGLGTLCISSIFNRDY
                     PVIQTYVLLIGVLFVMFNLLFDILQTVSDPRMRKESN"
     gene            complement(40549..42174)
                     /gene="nikA"
                     /locus_tag="ACZPCL_00405"
     CDS             complement(40549..42174)
                     /gene="nikA"
                     /locus_tag="ACZPCL_00405"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1160989.1"
                     /translation="MKKRWGTMLLAGLLGTALLSGCGSSASDSASSAAESNPSETTTT
                     AEGTKEDLIFVNYRDIRDLNPHLYAGEMYAQEMLYEGLVNITSDGFEGCLAESWDVSD
                     DGKVYTFHIRPGVTFSDGEKCDAYAIKANFDAILENKDRHTWLEMMHLLVGVDAPDEN
                     TFVIELSEPYYPLLTELGVTRPFAMISPKAMKNGSTKDGVEAYIGTGPYVLDEFVTDE
                     YAVFKANPNYWGEQPAIKTITVKVIPDNQTRILALEKGEIDMIFGKNMIDADAINQYL
                     DSDQFTVELSDPTSTRQIVLNTSSDILSEKDVRIALQHATNKQNISDGIFYGLEKPAD
                     TLFASTVPYCDIELEPYVYDLELAGQILDEAGWKMGSGNVREKDGQPLNIDLLYNSDS
                     VTEKSIAEYLQSEYQSIGVSLNIHGEEEQSYRDNMKAGNFDMVFNICWGTPYDPQSSL
                     AAMRAPVYGDYAAQLGLDDKAEIDDAITEILITTDENRRQELYTYVLTHLHEDAVYIP
                     LTYECNKAIYRSDMKGFHFTQTQYEVPFQDFSF"
     gene            complement(42330..43232)
                     /locus_tag="ACZPCL_00410"
     CDS             complement(42330..43232)
                     /locus_tag="ACZPCL_00410"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1160990.1"
                     /translation="MELRQLEYFMITCEKGSFNQAAECLYTTQPNVSKVISSLEKELG
                     RPLFERSSRGIHITPYGETVREYAQNILHNISLINSLTDHNQGKKFSLATYPSNMISR
                     LLTDFYKEMGNSYIVEHLEGTVEEISNRVAQGLSEIGIVYIAQKQLHQFQHILSHKKL
                     KFVPLDSKEACVYVGPKHPRYHDESIDFQDLSSLSFVRGVRDFFSVEHHLNHVSLGAI
                     STEQLHYAVYTNSDHLTVNLLLNTDICSLGINFLYDQYRQYDVRQLRINNCEPFLLIG
                     YICPDHAKLSKAAEWFICHFKEIL"
     regulatory      43449..43558
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            43741..45048
                     /gene="thiC"
                     /locus_tag="ACZPCL_00415"
     CDS             43741..45048
                     /gene="thiC"
                     /locus_tag="ACZPCL_00415"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR1160991.1"
                     /translation="MDYTTQMDAARKGIITKELQAVAEKEQMDTARLMQLVAEGKVAI
                     PANKNHKCLDPNGIGSMLKTKINVNLGTSRDCKDLDMELQKVNDAVKMGAESIMDLSS
                     WGDTQKFRRKLTAECPAIIGTVPIYDAVVYYHKALKDITTEEWLKIVEMHAQDGVDFM
                     TIHIGINRATAQRFKKNKRLMNIVSRGGSIIFAWMEMTGKENPYYEHFDEILDICQKY
                     DVTISLGDACRPGCLEDATDVSQIEELVTLGELTRRAWEKNVQVIVEGPGHMPLNQIA
                     ANMQIQQSICKGAPFYVLGPLVTDIAPGYDHITAAIGGAIAATYGAAFLCYVTPAEHL
                     RLPDLNDVKEGIIASKIAAHAADIAKGVKGASEWDRQMGMARKKLDWEKTFELSIDPE
                     KARNYRASAKPEKEDTCSMCGNFCAVKNMNRILDGEIVSIFDE"
     gene            45197..45841
                     /gene="thiE"
                     /locus_tag="ACZPCL_00420"
     CDS             45197..45841
                     /gene="thiE"
                     /locus_tag="ACZPCL_00420"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR1160992.1"
                     /translation="MKFDKSQLLIYAVTDRAWTGKMSLYEQTEAALKGGATMVQLREK
                     GLRDDNVEEYLEEAKKMRALTEKYNVPLLIDDNIKLAKACGADGVHVGQNDMDAGLAR
                     QLLGPDKILGVTAKTVEQALKAQSQGADYLGSGAVFGTSTKKDARPMTMEQLQAICAS
                     VKIPVVAIGGICLDNIEKLQGSGAAGAAIVSGIFAAEDIEGTTRALRQKMERII"
     gene            46226..>49549
                     /locus_tag="ACZPCL_00425"
     CDS             46226..>49549
                     /locus_tag="ACZPCL_00425"
                     /inference="COORDINATES: protein motif:HMM:NF019188.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoblobin-interacting domain-containing
                     protein"
                     /protein_id="HIR1160993.1"
                     /translation="MRKTGRTKALLAMFLAAASLQAGTAFGAQAATGSQLQAGTWVKE
                     DAGWKYRKPSGEMATGWVHTASGWYYLQPETGIMATGMLRINDRTYYFNQTPDAVEGK
                     MVTGWYQDAAGKWYFFSTTADSTEGAAVTGWQWVDGRSYYFEPAAGENTGKMFANGVT
                     PDGYKVNADGQWVDENGNIQVVPGKGFSSNPGKDTNSKTTVRGGGGGGSSHSSSGSTD
                     NGNNNANNGNTNNGNTNNGNTNNGNTNNGNTNNGNTNNGNTNNGNTNDSNSSNDNTNN
                     GNNSNNGNNQPDQNKAVFVKQDQTKLTELNSLGWWITVAFEDGYNAGNTKVYADGVDV
                     TAALSNVTDDGSICKLAVTKAPAVLTVSSNDDGAQVQTVSLDDTAADGIIYTGDSYLP
                     EKIMTHGPVSIWDYYLTNYADDGSVRYSPSKTTFALGEKPVAHPAYSPDAELSEDGKA
                     TVTIMFNYNTDDEKEWFDGINRLQLVEYNENKNTINSDLVFDRAKDVAHGSGRVGELT
                     IKSGQGNFTNNGRYYVRVESKAGTSALVPIHVVNAQAPVLKLQETAQSGVNLHFQVEN
                     MVYGIETPISRVVLEGPTETVELNEIDDWFLFSQDLFVLYNDNVDHLKYKGNYKLTVY
                     ADGFKATSVRFKVTNGENVPFKDEMGISQYSFDGVSTASTGSGGGSSSGESSSGSSAI
                     SANLVFDTDLLVNAELLEEAGRSTAESEAVVDWWYNIIKDAVFDRAEGKYYDWTDYVS
                     SVENKKYEDDQILSFEDYKRTGVTWSNSPATAKEVLEDGLLGEIQNNGDFARPAAGAF
                     TVEQNKEGENVVLKHENAQYLAAIKNVVINDSWTGLAKDKYTVDVEKGTLTLDQSLFT
                     PGEEYKIAVEADGYQMNAFKITYDQLLEEGLTLTVKYPDGKNEYASDKRTFGKIQGYY
                     ADAEVDVTGSEGGFLKYLTSVVLDEDDTLYTKGYEGSDAAYYEISADKKSLTIGNIKP
                     GTHTLTLRAKGYVDALTAQIVVAEGVDEPEVTFVPVVKSLEYKEAGFFMSPANYYITF
                     ENDTDTDAKKVLADYLDAVKSVTVGDVTYTKALLGLGYNNNEFAASVVDSGYGSINDC
                     LRMTVDTGFTGDTVEIVVEA"
BASE COUNT        14558 a        10071 c        12190 g        12730 t
ORIGIN      
        1 aaatagttga cattaatatt gacaacctat tgaaagatat gtatacttag tgatgcaaac
       61 aaatgttggc aatgttgaca atatataaaa aggagtcaag gttatgagcg aatgtttcta
      121 ttgtgaagac ggggaaaaga gaaagagttt aatgatcgag atctgcaagt taaactattc
      181 tacgatctac ttaaacagaa atcagaaata tccaggaaga tgtgttgtaa agctgaatga
      241 gcataagaca gaatacttcc agatgaccaa agaacagaga gatggatact ttgaagaact
      301 ggcagctaca gcaaaagcaa tctttgaact gtatcatcca gataagatca attatgcaac
      361 atttggggat ctggtacctc atgtacatgt acatgttgtt cctaaatata aagatggtcc
      421 agattggggt aatccatttg atgatacaca gggaaagaaa gaactgaccg atgctgaata
      481 tcagaagatg gtagatgaat taaaagaaac tatattaaag gaattaaata aataatttta
      541 ggaggatggc aaaaatgaaa aaaatgttag cagctgcttt ggcagcatct atggttatgg
      601 gacttacagc ttgtggcggt tcatcacagc cgacagaaac aaaggcacct gaaaatgcaa
      661 aagaaacaga ggcagcagca gagggaacaa cagaggctgc agcagccgga gatgaggaaa
      721 aaaagaccgg ttctgagatg acctggaagc tggctactga cgcagcaaaa gattatccta
      781 ctacaaaggc tctgatcaag tttgcggatg aagtttatga gaagagtaac ggacgtattg
      841 cagtagatgt ttacgaatct tctatcctgg gtgatgaggt ttcttatatg gagcagctcc
      901 aggcaggtac tgttgacgta gctaagttat caatcggtac attaagcgga ctgtatgaag
      961 atacacaggt attcttactg ccattcctgt ttaagaacaa cacagagatg tggaaagttc
     1021 tggaaggtga tgttggtact accattaaaa atggtctgaa tgattacagc attcagggaa
     1081 tcggtttcac agactgtgga agccgctgct tctatactac aacagaagta aagacaattg
     1141 atgatttcaa gggactgccg atccgtgtac agaataacca gttaatgaac tccatggtca
     1201 gcaacttagg tgctaaccct gtaaacgtag ctgcaaacga agtgtacagc gcactgcaga
     1261 ccggtgtatg taagggcggc gagaataacc ctaacatcat cctttccgac agcttatatg
     1321 aagttgctcc ttatgttctg atggacaacc atacaactac tatggacgtg atctgcatga
     1381 acctggatct gtgggagtcc ttaagtgatg aagataaggc aatcgtagac gaagcaatgg
     1441 cagatgcaac tgcatatgac agagaaatct gggatgcttc tattgatgag tctcttcaga
     1501 cattaaaaga caaaggtgca actattgtag aaccagatga cgcaacttta gattccttca
     1561 gagaggctat ggctcctatt tatgaagaat actctgcaaa atatgcaaca ctgttagatg
     1621 ccatcaatac aacattagga aaataataag ctggtttgat aattttaaag agggtatagg
     1681 cgatgaaaga aaaaattgaa aatgcgatcg ggcttgtata taccgtcgta gaagcgctct
     1741 gccgactggt gctgatcttt atggtatgta cagtaacagc gcaggtaatc agccgtgctc
     1801 ttggaggtaa tatcaagtgg tgcgaggagg tcatgctgat cctccttgac accatgatgt
     1861 tcttactgct tccggtgggc ataaaggatg atctgcatat tcgtattgaa gtatttgcga
     1921 aatattttcc aaggaaggta aaggtatttc ttctttattt cagcaatgca atactgttag
     1981 tagtatctct ttgcatgatc cgttatggat ttttcctgat ggaaaagaca aattctaaat
     2041 ttacgattac aggtcttcca agaagatatc tgtatctggt caccgttatc agtggaattc
     2101 tgtgcctggt catggttgtt gcaaagttct ttggtatgtt ccatacgaaa tccacagaca
     2161 atttcctgga atccaaaagc gagcttgaaa attaacaatg gtaaagtgat gatagaaaag
     2221 agggattaac actatggata caaatacaat agccatgatc atactctttg caacatttat
     2281 gatttttatg gttttgcgtg tacctgtaac attcagtatg ttgggtgcct gtgttctgac
     2341 cgccctttac ctgggaccga aatatgtatc atcggtattc ctgcgtttgg gtgacggcgt
     2401 acagtcattc tcatttttag caattccgtt ctttattttt gccggtgata ttatggcaga
     2461 aggcggtatt tcggaccgat tggtagaagt ggccaacgtt atggtaggtc gtttccgcgg
     2521 cggtcttgcc tatgtaaacg taatcgcttc aacctttttt ggtggtattt ccggttcacc
     2581 tacagcggac gtatcttcct taggaccgat cgagatcggt atgatgacaa aaagtggtta
     2641 tgacaaagac ttctctgttg caactacagt agcaactgct acacaggctc tgatcattcc
     2701 tccaagccac aacatgatca tttatggtat ggcagcaggc ggactttcta caggaaaact
     2761 gtttttggca ggtatcggac ctggacttat gttatgtgct gcaatgtgta tcctggtagc
     2821 acgtattacc agaaaaagag gttatccaag aggacctaag tatacagtcc gtgagtcttt
     2881 aaagattgtc agtgacggtt tcctgggtgt tatgaccatt gttatcattc ttcttggtgt
     2941 ttgtaccggt ttatttacaa ccacagaaag tgctgcaatc gcatgtgtat acgctttatt
     3001 tgttacctta tttatttaca agagcattaa agtaaaagat atctggagaa ttgcaaagaa
     3061 atccttggga accctggcta tgatcttaag tatcattgca gctgcaaatg cattctgcta
     3121 tctgatgagc cttatgcagg ttcctgcaaa gatcacacag atgttattaa cggtttctga
     3181 taacaagtat gtagttctga tcctgatcaa catcctgctg ctggtattag gctgcttcat
     3241 ggacgttgct cctctgatcc tgatcgtaac gccgatcctt ctgcctgtag taaaggcact
     3301 gggaatggat ccgatccatt ttggtatcgt tatcatgatg aacctggcag tcggttcttt
     3361 aacaccgcca gtaggaacta ccctgtttgc aggttgtgca gtaggtgata taccgatcga
     3421 aaaggctacg aaagcgctgc ttccatatta tgcagttatg gtagttgccc tgttgttagt
     3481 tacctacatc cctgcatttt caatgactct tccgaatctg ttaatgcact gataaagcaa
     3541 aggagaaggt atgataagag agtggaatga agaatcttta gatcatcgta atgcgttgct
     3601 gtatgcaatg ggaatctcag aagaagatgc aaaaagaccg gtgatcggtc tggtaaattc
     3661 atggaatgaa atgaatccgg gacattttcc ttttgataag acggtcatcc aggaaatgaa
     3721 ggatgagatc tataaagccg gggggcttgc tctcgaactt cctgttacag gtatctgtga
     3781 cgggatctgc tccaatacac caggagaccg gtatactctt cctgcaagag atctggtttc
     3841 cagtgaagtg gagatggttg cagaactgaa tatgttagag ggaatggtca ttatggctac
     3901 ctgtgacaag gtagtgcctg gtatgcttat gggagcattc agagtgaaca ttccaactac
     3961 aatgcttacc ggtggttata tggcagcagg ctgctatgag gaccgtatgc tgacactgac
     4021 ccacacaaag caggcttatg cagcttatgt ggaaggcgac atgagcagag aagaatacaa
     4081 agctattgtt cgccatgcat gtccaactcc gggagcatgt ccttttatgg gaactgccaa
     4141 tactatgtgt gccatggcag agatcctggg attttcccca catggaaatg cgtctgtaag
     4201 aagccagagt gagaaatggc atcagatggc aagagaagca gccagaaaag tagtggaggc
     4261 tgtgaaagaa gagaaacgcc caagtgattt tgtgacccag aagagtctgg agaatgtagt
     4321 ccgctatatg atggcgaccg gcggttctac caacagtttg ctgcatattc cggcactggc
     4381 aagacagatg ggatttgata ttacccctga gactttcgat gccatcagcc gtgaagtccc
     4441 gctgatcagt accatttacc caaatcatcc ggtatatacc atggaagaat ttgaccgtgc
     4501 aggcggactg ggtgctgttg taaaagaaat ggtaaaagct ggaaagattg atgcagacgc
     4561 agacggtatg tttggaacca tcagacagaa agcagaactg gctgaaaata tggatacaga
     4621 tgtgattcat ccagttgcag agccgatcag tgagcagggc ggtctggctg tccttcatgg
     4681 aaatattggt acagacagcg ccattgtaaa gtttagtgcg gtagcagaat ctgcatggat
     4741 ctttgatggt cctgctaaat gttatgattc acaggatgat gcatggcatg cgatcctgaa
     4801 agatgaaata gaagccggag atgtagttgt tatccgttat gagggaccaa aaggaagccc
     4861 tggcatgcct catatggaaa cctttatggc agctgtctta ggcaagggac tgggagaaaa
     4921 actggccctt gtatcagatg gaagattttc cggcgcaacc ggcggactgg ctatcggaca
     4981 tgtaagtccg gaagcttatg aaggtggaaa tctggctttg atccaggatg gagatatgat
     5041 ccatattgat atccgtgcca gaaagctgac agtagatgta agcgaggaag aatttgaacg
     5101 ccgcagaaaa gactggaagc cggtggaaaa gcctgctatg ggctggctga agctttacaa
     5161 gaataactgc acttctgctc acagaggcgc aaccatttac tgggattaat gttaataagt
     5221 ataatgtctg ataaataaaa acaggagcat ttaaacctaa cttcccttgg tttggatgct
     5281 cctgttttta tgatgtattt ttattgtagt tttataattt tggccagcac tttggcagct
     5341 tcgggttttc tacccggacc ggttccggat aagtttcacc gtaaagttct aattctacag
     5401 tttcaatgac ctgtggctct acaggtttat ttacttctac tcccgggatc gggaaatcta
     5461 cgggaacggt ctttacggct gcgatccggc ggatctcatt cataccggaa atgactttgc
     5521 caaataccgg ataggttccc ttatggtccg gacagtcacg cagggggaag aagaattcac
     5581 agcttgccag tcccgcttcc ccatatccgc ccatgcagac acagcctgga tgggagtcaa
     5641 gaggtgtaac atccgggttc agcgcaaatt catttgggat cagatactga agctccttat
     5701 ggccaaatgc cgtatagctt acatccaccc agttacccgg aacgatacgt tcgatggcgt
     5761 gatggtctaa gtagccgtgg gaagcagcgt agataaagct gtttactgtg tttggcgctg
     5821 cttcgggcag cagttctatt acaatatccg ctccgtttgc catgtggagt gtagcttttg
     5881 ggtttgtcat ggtgtgtcct cctgagatac tttactgctt attcaatgat ctgcaattcc
     5941 ttcggataat ggtttaatac ttccacacca tcttctgtaa ccagtaccag gtcttctacg
     6001 cggacaccgg tatcgccggt taagtagatc cctggttcaa tggagaagat catgcctggt
     6061 tttgcttccc atgtgttagc ggaggaaaca tcgccgaatt catgttcgga aaggccgata
     6121 aaatgtccaa ggcggtggtt gaagtttggt ccccagccct tggctgagat caggtctctg
     6181 gctgcagcgt caagctggta taacggtacg cctggacgga tctttgagat agcagtctcg
     6241 ttggatgctt tggtggtctc atatacttct ctgtgtgcat cgctgacagt cttgaaatag
     6301 aaggtacggg tcatgtcaga gcagtagccg tctttgatgc agcctacgtc aaagagaacg
     6361 cagtcaccag gttttacaac ggtatcgtct ggagaatgat gtgggtcagc ggcatttgcg
     6421 ccaaaggcaa ctaatggttc aaaggaataa ccgtctgcgc caagatctaa gtagatctga
     6481 agcatctggt cagctacttc tttttcagat acgccttcat ggatcaggcc tttgaattta
     6541 gccattgctt tatcgttgat agcagaggaa atacgcattt ttttctgctc ttcggtatct
     6601 tttacgcctc tggtgatatc aacggccaga gagccgttta cgaagcctgc agcggccttc
     6661 atttccataa gaggaagaag gaaacgtgcc tttaagtcct tgtctacgcc aagaacagca
     6721 tttttgtcca gacgggcagc gacgatctcc gggaccggat ctgtgtcaga ataccatact
     6781 ttttcaacac ctacatcacc aggcacattg aaaaggtggt ttaagaaata gtaatggtgt
     6841 ccgtcagctt ttaaaagcag tgcataaaaa cgctcgatag gagccatgga aatgcctgta
     6901 aggtagcaga tagacattgg atcggtaatg agcatctggt ctacgccctg ttcttttaag
     6961 gcagccagaa tgcggtttgc tcttgcttcg ttcatgataa tatttctcct ttatttaggt
     7021 tataaacata tgacgccggg gcttgcaaat agccttccga cgtcataata ctttactata
     7081 ataatgttgt caagcaacct tactttctgc atgcctgcac gcaggaaaga tgagaaagat
     7141 tgccattaaa actgagttct taattttgga tctaacaggt ctcttaaacc atcgccgatg
     7201 aaattggcgc caactgccat agtaaaaatg gcaagtcccg ggaaaccggc aacccagaat
     7261 ttattgaagt aatctacacc atcagaaacc atcatacccc attccggtgt tggcggagcg
     7321 gagccaagtc ctaagaagct taaggtggag aacatcagga tcttgtttcc gatgtctgtg
     7381 gtagccatga tcaggacgga gctgacgctg tttggaatga tctctttcat caggatacgc
     7441 atccgggaag cgccaagggc ttctgctgca ttgacatatt cgttttcctt tacgcttaat
     7501 acaacgcttc gcatcagacg tgcgtaggta ggccaggaaa cgattaccag ggcaaacatg
     7561 gtgttgaaca ggttagaacc cataactgcg ttgatgatca ttgccaggat cagggatggg
     7621 aaagacagga tcatttcgga aaaacgcatc atcacgttat ccagccatcc gcctacataa
     7681 ccggccagcg caccgtaaaa ggttccgata atacctgcaa tgataacaga cagacaccct
     7741 gcaagaaggg agtaacgtcc gccgtagatc acacggctga aaatgtcacg tccgaagttg
     7801 tctgtgccaa accagtggat ggcagaaggt gcctttaaac gcaaggacag atcctgtgcg
     7861 atagggtcat aaggagcaac aacaggtgct aaaacagctg ctaagatcca tccaagacag
     7921 atcaaaatac ccagtgtaaa caggtaatta gattttaata attttttcag ggaatggatc
     7981 atggttaatt gcacctcact cttgggtcga tgatgccgta gaggatatcg accactgtat
     8041 tgatgatcat ataatttaat gcgatcagca gtgccacgcc gatgatcgcc ggatagtcag
     8101 ctgccataac agactcatag gcaaactgtc ccacacctgg ccatttgaaa atggtttcta
     8161 ctaataccat accgccaagg aggtttccaa gtcccagacc gataacggtc ataaccggga
     8221 tcagggcatt tccaagggca tgttttaaga tcaggcagat actgtttaag ccttttgcct
     8281 ttgctgtgcg gacatagtct gtagacaggg cgtctaacag gttagaacgg gtggttctgg
     8341 tgatcaggcc catggtaaag gctgctaaaa cgatacttgg aaggatcagg tgacggacgg
     8401 catccatggc ttttgccgga tttccttcga tcaggctgtc gatcacatac attccggtaa
     8461 cggaagccgg agcagagaaa gaattgctta aacggcccgg tcccgggaat atttttaact
     8521 tatagtagaa gaagtacagc atcagcagtg caaaccagaa ggccggaatg ctgacacctg
     8581 ttacggaaat ggcccggacg atctgatcta agatactgtt gcggcggatg gcggaaatga
     8641 taccaaacag gatgccgaaa acagaagcga tcacgatgga aaacagggcc agttccagag
     8701 tggcgggata acatcttgcc agatcgtcca gtaccggacg gctggtacgg atagaggtcc
     8761 ccaggtcaaa gtgcagaaga tcacggatgt aataaatata ctgcacgatg agcgggcggt
     8821 ccagaccgtg ttttgcacgg taggccgcta cgatctccgg atcatttaag gctctctggc
     8881 ttaaatttgc aaccaccggg tcgccaggta tcatctttgt aaggatgaat accagggtgg
     8941 caacgccgaa cagcataact gccaaataaa ccacacgttt tccaataaat ttcaactctc
     9001 ccatttggat ctggtacctt tctaaactat ctgatgtaag ttgcctccgg cggcggtgcc
     9061 gcgggaaaac agcagatttt gatattttga gtgcccttgt tggggcaaga tcatgcttta
     9121 tgcccccaat gggacaaatg cagattttca tgtttcagat gcccccgcaa aggggcacct
     9181 ggatttttgt tttattactt tacgttgatc tcagtcagat caatagtgta ggagtcaaaa
     9241 atatctaccc catctaatct gttgctgtga gcaatatgag caggagcctg cgcaattacg
     9301 atgaatgggc cgtcttcata cattgcgtcc tgaatatttt ccagaacctg ggtgcgggca
     9361 tcattgtcag ttgcttccat tgcttcctgg tacattgcag ccagttccgg gttcatttca
     9421 gcagtccatc cggctctctg tcctacagta ccgcctggaa ggaattccag ctgtacgtta
     9481 ggatcgctgt aatctacgcc ccagtacata acagtaaagc caagagttcc gtttctgtaa
     9541 gcgtcgccgt agcctgctgc ccatgcttct gttgtgatat ttacattaat accgatctgg
     9601 gataagtcat cttttacctt ctgggccagg tcagttaaaa ggatgccttc catatccagg
     9661 tcagaaacag tcatatctac gtcaaagccg tccggataac cggcgtcagc taacagctgc
     9721 tttgcttctt cgatgttagt gtagtcatct gggcgtgttc ccttgcttcc cataaatccg
     9781 tcctgaatga tagaatatgg agttaaagtg ccgtcaccgc agatcatgcg gattccggag
     9841 taatctaaag ctttgcggat ggctttctgt acatctgggt tggaaacagg tccgccgtat
     9901 tcttcgttca tgttcatcat aacgaaaccg atcatctttg tggttccgtt tacgatctct
     9961 acattttcag cagatcccag ttcctctaag gtatcatctg tcagatttaa tgcaacatca
    10021 atatctccgg tagacagagt catcatctgg gtgttagcgt ctggctggat ctttaaaatg
    10081 tatttgtcta cgttagtaga agtgccccag tagttcgggt tcttttccag aacaacttcc
    10141 tcatcaggag tgtagctggt caggatgtac ataccgcttc ctgcacttgc attgtcaagg
    10201 aaactctgtg cagtatcagt ggaagaagca tcttctgcgt cagtaccgcc attttccttt
    10261 acaacagcgc tgtcaaggac agccagagaa ctgtaagtca gcttggaaag aatggcactg
    10321 tcaggctgat ttaagtggaa gattacagta gcatcatccg gggtttcaat gctttcaatg
    10381 gtatcgcaga tgaaagaagg atttcccttt aagttcttgg tacggttgat gctgaatgca
    10441 acgtcagcag aagtgatctt attgccgctt gcaaaagtca ggccgtcttt taacttaaca
    10501 gtcagtgtta agccgtcctc agaaaactcg taagtatctg ccagacaagg ctgtggtgtg
    10561 ccattatttt catagaattt aaacaggttt tcatagcagg catttacgat cagctgtggg
    10621 tacttttcgt aaacataacc tggatccaga gtggagaaac cggaacccat ggcaacgata
    10681 acggtattgt cgccggtggc actgctttct gcctgggtgc tgtcagaaga acctgctgca
    10741 gaggtgtctg tggatgcctg tccgccgtcg catgctgtgc agcctgctgc aactgccatg
    10801 gagagggcga aagctaagag ttttttgttt ttcataatta aatcctcctc ttaaaatagt
    10861 aaatattgat acagcttttg aaagctgcga taaagcaata ttttatgtgc tggttaaatt
    10921 catttactgg tataaatgac atgccacaaa atggcctttt tcgatctctt ttaactgcgg
    10981 gatctctttg gagcagatat cacaggcttt cggacagcgg gtgtggaacg ggcagccgga
    11041 aggcggatca gaaggacttg gaacttcacc tgttaagtgg atgcgttctg tcctgtcatc
    11101 tgcgtcaacc tgggggatag cagataaaag agcctctgta taaggatgat aacgtttctc
    11161 atacaactgg tcataagcgg cgatctccac catttttccc agatacataa ctcctacgcg
    11221 gtcgctgacc tggtaaacaa cgtttaagtt gtgggagatg aagaaataag tcagtcccag
    11281 gcgttccttt aattcctgga gcaggtttaa gacctgtgcc tggacggata cgtcaagggc
    11341 ggaaactgcc tcgtcacaga taataagctt tggctctaaa gccagcgctc ttgcaatacc
    11401 gatacgctgt ttctgtccgc cggaaagctc atgaggataa cggcttaaat gataggtgtc
    11461 aagacctact aaatgaagca gctcctcgat ccttgcatct acatccatgg aaccggtcat
    11521 atggtggatc tggaacggtt ccataaggat ctggcgcact gtacgtcttg gattaagaca
    11581 tgcggacgga tcctgaaaaa tgatctgggc tttcttacgc atctgtttta aggctttgcc
    11641 ttttaatccg gaaatatctt ccccgtctaa ataaacatgg ccgtctgtag ccgggatcag
    11701 gcggatcagg gtgcgtccca gggtactctt tccgcagccg gactcaccaa ctacaccaaa
    11761 ggtctcacct gcgaaaatat caaatgaaat atcgtctacg gcacgtactg tgccggattt
    11821 cttgtctttt cctttaaaat atacttttac atggtctgcg tggaccagta cgtctttgct
    11881 tttgttttca tcagccattg gttactgcct cctgacttcc attatctgcc tgtgcgtaga
    11941 gccagcagcg tacctgacgg tcttctccaa cagaaaatac cggtggttct tccttacggc
    12001 agcggtcgca ggcttttgga catcttggcc agaaacggca gcccttgatg cgctctaccg
    12061 ggtttggaac cataccttca atagtggata atggtttgcg ctctttggtg atccttggga
    12121 tagcgcttaa aagtccctgt gtgtaaggat gaagcggttc gcggaaaata tcagctgtag
    12181 gagccttttc tacaatgtgg ccgttataca taacgataac tgtatcgcaa agctcactta
    12241 caacaccaag gtcatgggta atgaacacaa cagaggtgtt catttcctgg ttcagatcac
    12301 ggatcaggtc taagatctgt gcctgaatgg tcacatccag agcggttgta ggctcatctg
    12361 cgatgaggat ctgtggctga caggccaggg ccatggcgat catcacacgc tggcgcatac
    12421 cgccggacat ctggtgggga tattcataag cacgggcctg tggattggcg ataccaactg
    12481 cctgcatcat cttaatggct tcttccatgg cttctttctt tttcatgcct ctgtgaaggc
    12541 gtaaagactc tgcgatctgt tttccgcagc ggataatagg atttaaagaa gtcataggtt
    12601 cctggaagat catggagatc tcgtttccgc ggatcttctg catttcccgt tcagaaagtt
    12661 ttaccagatc ctttccatta tataagatct gtccgccggt gactttgccc ggaggatttg
    12721 ggatcagctg catcatagcc agggaggaca cactttttcc gcttccgctt tcccctacga
    12781 tgcctaactt ctcaccgcgg tggagcgtat agcttaagtg gtctactgcg tgtaccgttc
    12841 cggccatggt cttgaattcc acacacagat cttttatttc aagtaatatt tctgactggt
    12901 tcatggtttt ctctcgtttc tacaggattt tattaataaa cgaacatatt atccgactac
    12961 tatgctggat gttagcaata cgtttgtcag tttaaagcga caaagtatca acgcaaaaaa
    13021 aaataactat atatagtaca gatacaattg ttaaggctta gaataccata ttttctaagg
    13081 aaattcaact ggggaaatga tgttcttttg atatttgttc atctattctt cattgaaaac
    13141 aaacgttagt ctggtataat ggtatcacat agttacaaac gaagaatatt taattttatg
    13201 aggtttacat atgaaacgaa aaacagaccg ggggagaatg aaatttcggg cgtttatggt
    13261 ctgcgttctt tcttttctgg tcgttacagg cagtgcctgt tttttaaacc gggaacagga
    13321 aaaggaagaa aaattaaagg ctgtttatgc agcggagtcc acagtaagca gggtaaaatc
    13381 ccagttgaac cggtatctgg cggagtcaga actggtgaaa aatatcatag agtcaggata
    13441 tgatattgat ggagaaaaat tttccatttt atcccagatg atgcaggata agcaaaatgt
    13501 gatcgaggcc catgaactgg caaaggacgg tattgtttcc catatctatc ccatgaaagg
    13561 gaatgaagag gccatggggt taaatatgct ggagcatccg gacagaaaga aagaggcaaa
    13621 tctggcaaaa acaagcggtc agtataccat tgcaggaccg tttccactgg tccagggcgg
    13681 aaacggcgca ctgctttttg atcctgttta tgtaaaagat gaagcagggg aagatacgtt
    13741 ttggggcttt tccattctgg tattaaactg ggatcatttt atggaagaag tggaaacata
    13801 taaactggaa gatgcgtcct atcagtatct catatggaaa aatggcacag aactgggaga
    13861 aaagctgacg attgcccaga gtgaaaaatt ttcctttaag gacacactgg aagtagcctg
    13921 tgatgtgcct aatgatacct ggtattttga gatcgagcca aaagccggat gggtaccgca
    13981 gtcacagatt gcttttggga tactgatcgc agcccttgtt tcaggagtgt tgacggttgg
    14041 ttactggcag tatgagatgc agcgttataa agaggcactg tatgcggaaa agatcgaacg
    14101 ggcagcaaaa cgggcggaag aagcaagtga agccaaaaca agatttctct ttaacatgag
    14161 tcatgatatc cggactccga tgaatgcgat catcggtttt tctgatcttt tagagaaaca
    14221 tctggatgat aaagaaaaag tccatgatta tattaagaag atccagttat ccggttcgtt
    14281 tttattatcc ctgatcaact atgttctgga aatggcgcgg atcgaaagcg gaaaggcgac
    14341 attacggaca gaagttgggg atgcccagga actgttaggg gcattaaatg cagtttttga
    14401 accggcagtg gagaagaagc ggctgaaata caactgtacg ttggatgtag aacacaggtt
    14461 tatcatctgt gatgtgacaa aagtgcggga gatcgtatta aacattatca gcaattctgt
    14521 taagtacacc ccggaaggcg gcagcgttac agtacagatc aaagagatcc catgggagaa
    14581 agagggatgg actgcatacc gtattctggt agaagatacg ggaattggca tgggtgcaga
    14641 atatctgcct catatttttg aagaatttac cagagagcgg accagtacgg aaagtaaggt
    14701 agtaggtgcc ggtctgggtc ttccgatcgt aaaagccctg atagatctta tgggtggaac
    14761 gatccaggta gaaagcgaaa ggggcaaagg cagcaagttt gaagttattt tgccttttga
    14821 gatcgcttct gaggaagagg tcaaagacag ttatgtaaag aaagaagaaa agctgtataa
    14881 cagatctaag gagaagcgca ttcttcttgc agaagataat gagctgaatg cggaaattgc
    14941 cataaccata ctggaagaaa atggatttaa agttgagcgg gcagaggacg gatgtaaatg
    15001 tgtggaactg ttttcagaaa agccggcagg ttattacagc accattttaa tggatatcca
    15061 gatgccaaat atggatggct atacagcttc aagaaagatc cgtggcatgg aacgggaaga
    15121 cgcaaaggcg atcccgatta ttgccctgac tgccaatgct tttgatgagg accggaataa
    15181 ggcgtttgct gcaggtatga acggacatat tgcgaaaccg attgatgtgg ggaggatggt
    15241 cagatccatt ggagcgttag taaaataagt gttagtgaga taaatgtcag cccaacagaa
    15301 aaggatattg tcaggcggta taaaaacagt ctggcaatat cctttttagt ttccatgcat
    15361 ttgcaagaag ctgccggtgg caggttctgt aaatgagggt gtaagttata tgagttacat
    15421 gatcaaactt aaagcggctt catctgcgat cacggttaca tcattgtgca gctgtaagat
    15481 agatgccgga acagatggtg taatagggcc atagagagag ttctttaagg cttctgcctt
    15541 ttcctcgccg cctgcgatca gcaggatctt ctttgcctgc atgatgtttt tgatgcccat
    15601 ggtataggcc tgttttggaa cgtcatcttc agaggcgaag aaacgtttat ttgcttcaat
    15661 ggtgctttct gttaagtcta cacagtgggt ctctttttca aaagcagcgc caggttcgtt
    15721 aaagccgata tgtccatttc ttccaagacc taaaagctgc agatcgatac cgccgtggga
    15781 gcggatgatc tcattatagg cagcgcaggc tttctgtgga tccggctcta agccgtctgg
    15841 aacgaaggtg cgggccttgt cgatatttac atggtcaaac aggttggtgt tcataaaata
    15901 gcggtagctc tggtcattgt ccgggtcaag tcctttgtac tcgtccaggt taatggtggt
    15961 gactttggca aagtccagat cccccttgtt gtaccattcg atcagctgtt tgtaagtgcc
    16021 aatcggtgaa gaaccggttg ctaaccccaa tacgcagtcc ggcttcataa tgatctgggc
    16081 ggaaatgatg tttgcagcct tgcggcttaa gtcctgatag tttttagctt tgtagataac
    16141 cataagtgtg tcccctttct ttgttgcttc tccttatgat accagtatac ctggtttggc
    16201 gggaaattca atagaaagga ggcaagaaaa aaactgacag ggatgtagaa gtatggtatg
    16261 ctaggaaaga gtggaccatc tgcgtcagca gacataaaaa acgtgccagc gcagggttca
    16321 taagtctata ataaccacca caacggagga ttaaatcatg gaaatcacag gacgcaatgt
    16381 aggaaagaca tgccgcaccc attataaagg tacttttaat gatggaaccc agtttgactc
    16441 ttcttatgac agaggcgaac cattagagtt cgtatgcggc gcaggccaga tgatcaaagg
    16501 ctttgacgca gcagtggctg atatggaagt aggagaggta aaggatatcc atctgatgcc
    16561 ggaagaagca tacggccagc caaatccgga cgcgatcttt gaactggaga tcgagcagct
    16621 tccaggttct gaggatctga ctgtaggcca gcaggtatat ctgaccaatc agatgggcca
    16681 gccattccct gtaaaagtta ctgcaaagac agagaagacc attacttttg acgcaaatca
    16741 tgaaatggca ggaaaagagc tgaactttaa gatcgagctg gtagaagtga aataataaaa
    16801 atttaatttc tggacggaag taaatataga aagcagcagt gtatatttta cccgcatttc
    16861 cacatactac ccataagaca cacaatatgg aaacagagat gttttgtggt atgcaggagg
    16921 aataaacgtt atgaaagcta caggaatcgt aagaaggatc gatgaccttg gacgtgtggt
    16981 gatcccaaaa gagatacgaa ggaccttgcg tttgcgggaa ggaacacctt tagagatatt
    17041 tacagaccgt gagggggaga tcattttaaa gaaatattcc cctatggtgg aactggctgc
    17101 ttttgccgga cagtatgcag aggccatgtc ccaggcaacg ggcctgatcg tgtgcatcac
    17161 ggaccgggat caggtgatcg cagtatccgg cggtgccaga aaagaactgc tgcaaaaagc
    17221 catcagccct tctctggagc aggtgatcgg tgaacgccag gtcgtgcaat ccggaaaaga
    17281 tgaaaaaagc tttgtccagc tggtagatga agaattagaa ggtattaccg cccaggtggt
    17341 agcccccatt atctgcgaag gcgatgctat cggcgccgta gccattatga gccgggagcc
    17401 aagagcgcgg tttggagaaa tggaaatgaa gctggctgcc actgcctgcg gatttttagg
    17461 acggcagatg gaaagctgaa acaaagaaat atggatcagt aataggaata ataggaacag
    17521 caatgcaaaa gcccccaggc aaaacctggg ggcttttggc aagagaaaaa agttaagtac
    17581 ctaatatgag aaagggcagt cagtcccttt tcatcagatt aaaaatgtac agaaggtttc
    17641 agagaatacg cagcagaaga agactgccac atatccaatg aggaatggga tctctgcttt
    17701 tgcaatatcg cccattttca gaccggatac ggtagaagcg gagaagatgt ttactgcaac
    17761 cggaggtgtc atggtaccaa ctacgttgga gatgcacacg atcatgccga agtggatcac
    17821 gtcaatgccc atactctggg caataggcca gaggaccggt actaaaagaa cggcaatcgc
    17881 ggttccttcc atgaatgtac cgaagatcag caggataatg gcaactacga agcagaacag
    17941 cgcggaattt aaatgcatgc tgataactgc gtttaccagt gcctgggaag cgcctgagaa
    18001 ggtaaatacc caggagaaag cggtagaagc tgcgatgatg aataagatca tggcagagct
    18061 tttcgcggaa tcttttaaaa tggggaccag ttcttttaca gtcagttctt tgtagatgaa
    18121 cagtccggca atggcagccc atacaactgc gatggcagca ctttctgttg ctgtcagcat
    18181 accggagtag atgccgccaa ggatgatgat gggggtcatc agcgccggga tggcatctaa
    18241 gaagatctta aagatctcag aagggctgcg ctttacagcc tgttttggcc agttttcctt
    18301 ttttgcgtaa aagagaactt ctgccattaa tacaagcatg atcacaatac cgatgagcat
    18361 gccctgttta aacatgtttc caacagaagc gcctgttaaa gtacagtaga tgaccatgat
    18421 gatagatggg gggatgatcg gtccaagacc accggataca actaacagtc cggctgtacg
    18481 gtcacctggg tagccaagct ttaccatatc attgtagagc atggtaccga tagcaataac
    18541 agttgcagga gcggaaccgg ataaggctgc gaagaatgcg caggctaaaa ccagtgccag
    18601 gctcattccg ccgcgcacat tgcctaccag gcagttggcg aaatttacga tacgacggga
    18661 gataccacct ttggtcatca gatttccggc gagtacgaag aaagcgatgg ccataatgga
    18721 agtagactgc agtccgccga agatacgctg tgcaataacg gtggaggaag cggacagacc
    18781 gccgaatact acggagcaaa gggaagccac tcccagggag atggaaaccg gaactcctac
    18841 aataagacat acagcaaacg ctgcgaaaag gatgatacct gcactcatgc ttctgcctcc
    18901 ttattcttta aatcaatgat cttttctacg aggatcacag tctggatcag taagatcagt
    18961 ccaaaggtaa gcactagtga gaaatacatg gcagacatag ggatctttaa tactggagtt
    19021 gtctggccag tctggatctg ttttaaaaat aaggcggtgc cagcctttaa cataacagcg
    19081 gtaaaaatct ctaatacgat ctgttcaaca atggagacca ctttttttgt gattccgtga
    19141 agcttttcaa taatagcggt aacagcaacc tgtgtgccgt cacgaagacc tacttctgtt
    19201 cctaacagtg ccatatagat catgctgtac atggctacct cttcagtcca tggcatggaa
    19261 gccttgataa agttgcggtt gacgactgct acaaagtagg cagttaccat ggcgacaaac
    19321 gcaacgatca taatggcata ttctactttt gtgacatagg acagcagctt tttaatacct
    19381 ttcatagttc cctctccaat ctcattttcc gacctctttc agagcatgtt ttcagttgtc
    19441 tgccagcaga tgattttgat gcaaacattt tgttgcagtt cagtcatgac atcttcttgt
    19501 ttccatgttc atgaaaacaa gaagcatggt ggttcaccat atgctgattt gtatgagaaa
    19561 gcgcttatgc aagcaggctt gcaacacact ttttcataca aaccagcgca tgactgaact
    19621 gtaacaaaat aataagatgt ctgatgtaat taaaatacca catcagacat cttacgccaa
    19681 tgtattatca gaaaattaac atgattttaa tatcaaaaag atgatattat atttattaac
    19741 gacattttaa caacaattaa tattgttttc aaacaaatct aattgctgct gccattttgt
    19801 atcagccact gtgtaaaata atttcggatc acactgaaag gtgccggacc tatatccagg
    19861 agaaagcggt taaattctaa cagggagaag ttggctccca gtctttgctg tgcttcttct
    19921 ctcatattta aaaattccag gtaaccagtg taataggaga ggtagttgga aggattttcc
    19981 actacttcat agtaaatgga agagatgaca gaaggatcat cgatctggaa gtacatagtc
    20041 agataatcgt ttacctggtc catatcccag ccctcgtagt ggatgttaat gtccaacagg
    20101 gcataaaggg caagtgtaaa ggcggcattg tgctgcaaaa gttcacctac ctgctcctcc
    20161 atgccatgat ccagtgtgta ggcgtagtat tccgcataag ttccccagcc ttctacatat
    20221 ccctgaaaat caaggacatt ccgcaggtta caggtgttat gctggttaaa gtaattggtc
    20281 tggtacatat gtcccggata accttcatgg gccatggtgg tgtagaggga gccggcatct
    20341 gtactctggt tattaatgta aatggaattg tcttccggcc ggtcaatagg tactgtaaga
    20401 tagaaagcag ggcttaaaaa gccttctaat gttttcggca catttttaat ggtatagtta
    20461 cagtcattta caggcgggaa atctgcctgg gactgggctt tcagatcctc taaaatgtcc
    20521 ttaggttccg tcagggtaaa ggtggaagag tccagtttat cgcctaaaga cggatcatcg
    20581 cttaacagct ggttcacggc tgccagttcc tttgacatct gcttgttaat ggcctgtttt
    20641 aaggcagaaa catcactgta ggaggttccg gtgctggcat ttaccagata ttcataatat
    20701 ttccggcctt ccgggtagta gtaaagaccc ttgtcattgg aacctgttcc ttttaaactc
    20761 tccagaccgc tgatcagccg ttcataggca ggtacgaaat gttcgtcgat ggctttttta
    20821 ttttttgcct tataatcggc tttttcctgg tctgaaagac cgcccatttc atctaaacgg
    20881 gaagaaaaag tctctgccat aaagctgtgg tctgcgtcaa taagataagc attgcaggag
    20941 cggatgatat tgtcgataac tgtatcacac atgccaagat ccgcctgggc ccgctctgtc
    21001 tcaaaggcaa tgagagagtc ataataggtg tcaatggatg acagaagaga caggtaatca
    21061 tctacatcct gtttcctgta aaaagtatat tcagataaaa ggatagggag ttgggactgg
    21121 atgccaaggg tggtggataa aggctggtca tacagctcta atccatcaga ggacagaaga
    21181 gtattaatgt aggaagaaag aatggtatag gtaagcagct ggtctttgga taaaagacgg
    21241 gtatccatag cttcaaggtc tgctttgatc tgttttgcct cttcaaggtc ctttcgtgta
    21301 tcttcagcgg aagcagttcc aagagtgcgg tcataatcgg taatgccgta actttccggg
    21361 tctgcaagtg tatagtgaag ggtaagaagg gactgggagg cttcctgttt aaacaggttt
    21421 tccgtgtact ggtcaaaggc ttcccgggct gcagccaggg ctgcttcgtc tggctggctg
    21481 gcggagtcgt cagaagagtt ttcagttgct tcttctgcaa tggtttcctg agaggtattt
    21541 ttgtctgctt tcggtgcaga atttggaaaa agtgagcagc cggtaataag gcacagggat
    21601 aacaccagtg caagaatggc atgcagatat ttttgtgttc tctgtttcat aaaaatctcc
    21661 tttttgggat acaatgatcg tccatgatac cggattgcta cgtgctttgc actctagcat
    21721 cggattttag accttttgac actggtgtca ttatatatta tatgtaggaa aattacaacg
    21781 tatgtggcat agggcgtctt gacgaaagcc ctgtaaacgc ttatacttac atcattagaa
    21841 tgccccaaaa gggggctgga aagagggatt taaaatatgt gtcaggattg taagaaaaaa
    21901 ccgtattata ttacaactgc catcgcatat gcttccggta agccgcatat cggcaacacc
    21961 tatgagatcg tgctggcaga cagtattgca agatataaga gagctcaggg atacgatgtg
    22021 ttcttccaga ccggtaccga tgagcacgga cagaagatcg aggagaaggc agaggctgca
    22081 ggcatcactc caaaggagtt tgtagataag gctgcaggcg agatcaagcg tatctgggat
    22141 ctgatgaata cttcttatga taaatttatc cgtaccacag atgctgacca cgaagcacag
    22201 gtacagaaga tctttaaaaa actctatgac cagggagata tctacaaagg tgcttatgag
    22261 ggactttact gtactccatg tgagtctttc tggactccat cccagttagt agacggcaag
    22321 tgccctgact gcggcagaga ggttaagccg gcaaaggaag aggcatattt cttccgtatg
    22381 agcaaatatg caccgcagct gatcgagtac attaattctc atccagagtt tatccagcct
    22441 gtttccagaa agaatgagat gatgaacaac ttccttcttc caggacttca ggatctgtgt
    22501 gtatccagaa ctacctttga ctggggtatt ccggtaacct ttgatccaaa gcatgttacc
    22561 tatgtatggc tggatgcgct gaccaactat attaccggtg tcggctatga ctgtgatgga
    22621 aacagcactg accagtttaa gaaatactgg cctgcagacc tgcacctgat cggtaaggac
    22681 attatccgtt tccatactat ttactggccg atcttcctga tggctttagg cgtaccgctt
    22741 ccaaagcagg tatttggtca tccatggctg cttcagggcg atggcaagat gagcaagtcc
    22801 aagggaaatg ttctctatgc agatactctg gtagatttct ttggcgtaga tgcagtacgt
    22861 tacttcgtgc ttcatgaaat gccatttgaa aatgacggcg ttatctcctg ggagctgatg
    22921 gtagagcgta tgaactctga cctggcaaat atccttggaa accttgtaaa ccgtaccatt
    22981 tccatgagca acaagtactt tggcggcgtt gtagaaaaca agggcgttgc agaagctgtt
    23041 gacgaagact taaaggctgt tgtattagag gaagtaaaga aggtagacgc aaagatggcc
    23101 cagctgcgtg tagcagatgc cattacagag atcttcggta tcttcagaag atgcaacaag
    23161 tacattgatg agacaactcc atggactctt gcaaaggacg aggctaagaa agaccgcctt
    23221 gcaacggttc tttacaacct gacagaggcg atcaccatcg gcgcttccct gttagagtca
    23281 tttatgcctg agacttctga gaagatcctg gctcagttaa atacaaagaa gagagagctg
    23341 gatgaaatgg ataccttcgg tctgtatctt tcaggaaata aggtaacaga caagcctgag
    23401 atcctctttg cacgtatgga tatcaaggaa gttatggcaa aagtagagga aatgcgcgca
    23461 gctgcagcag aagctgcaaa acctgcagaa gaggcagctg aggatgatgg cattgactta
    23521 gagccaaagg cagagatcac ctacgatgat tttgcaaaac tgcagttcca gatcggtgag
    23581 atcgttaagt gcgaagcagt accgaagtcc aaaaagcttc tctgctccca ggtaaagatc
    23641 ggaaccaaga cccgccagat cgtaagcggt atcaaggctc attactcacc agaggaaatg
    23701 gtaggaaaga aagttatggt agtaaccaac ttaaagcctg ctaaattagc cggagtcgta
    23761 tccgaaggca tgatcctgtg tgcagaagac gcagagggaa atctgtcctt aatggtaccg
    23821 gaaaagaaga tgccagccgg agctgagatc tgctaatgat cagtaaataa atgtatttca
    23881 tgtttgtaat agtagaaatt tgggcgcata taattgaaat ataaagagat aaagggagag
    23941 ggaaaatatt cccctccctt tttcgcgcat atactggaca ttttaagaga aaactgctat
    24001 tctgaatata agcaggattt caaatatatt ctaaatattc tatatttcta agctgtatcc
    24061 aaaacagcat cttatctgaa actctttgct taaatcacaa accaatgaaa caggcagaga
    24121 gtacagagaa aactttctgc cgactaacag aaaggacgat gcctatgtca aacagtgtat
    24181 gggaaccgga aataaccgat catgagatgt agctggaaaa tagggagttt aatctctgtg
    24241 attttgcctt atttttatct gttatgaaaa acaaaacagc acacagaaat gttttaagta
    24301 ttataacagg ggaaaaaaat ttaaaactga aggaagtata tgtagaagag gtgatcctga
    24361 ataagagcgg gcagcgggca atccgtttag acgcaagggc aacagacatt tcaggacgca
    24421 attttgcaac ggaaatgcaa aatgatacag agcaggatga tatgcggcgc agggcgagat
    24481 tttatcaggg actgatcgat acgcctttat taaaatcggg aagaaagacc cggtataaac
    24541 atctgccacc tacgatcatt acatttatca cccagaagga tatatttgga aaagggctgg
    24601 caaaatatac ttttacagag cagtgcgaag aaataaggga cttgcatctg gaagatggaa
    24661 ctaaaaagat atttttaaat atgaccagca aaaagggaga gcctgtatta gtaagcctgc
    24721 tgcagtatat gaaacatacg actttggaaa attctgaaat tttagtaaaa gatgagcgca
    24781 tagcgcaatt agatgagatt gtgacagaga taaagcaatc agaagaatgg gaggctgtaa
    24841 ggatgagcat tttatctatc ggtttggaac gtggaatgaa gaaaggagtg gcttggggaa
    24901 tagcgaaaag tattttggat atacttgaag aaactggtcc cgtatcggag aaaacccgtg
    24961 aaatgatctt gactgaaaca gatgcagagc gtctaagcgc atgggttaaa aaggcagcca
    25021 gatcgaattc ggaagaggat tttttaagta agatttgatg tgcctaaaag aaaaataagt
    25081 gacatagatt tacgcaaaac agccccacga aaggggctgt taagtttgaa gtatgtgttg
    25141 aaagttatca acaaaatctc tttacagtcg gaaccagcac tttctgcata ggtttcatca
    25201 cttttccaac taacagggcg cacagtacag ttccttcccg tattccctga agttctccaa
    25261 ggaaaacaaa acacaggaca atacctacaa caactgccgt acaatccagg atcactttta
    25321 cttcatgaaa ggctttttta ggaaaaacag ttttattcat ggctgcggta aggccttcca
    25381 taggcatgac gatcaggtcg gtgtccagat acaggcagat gccaaaagca acgaggaaaa
    25441 tactgatggc aagcattaca agccttccgg gatagccggg aaggcagaag cttccaagaa
    25501 gggtttctgt caggtcaacg aaatatccaa aggcaaagga gcagaggatc tgggcaagat
    25561 ccagagggcg gaaagccttt cttaaaagca ggatctggat caggatatag ccgataaaga
    25621 taacggtcac acaggttcca aggtcttttc caaggatccg gcttaccaca tagggcagtg
    25681 aattgaccgg agatacaccc agattggaat tgacggaaaa tgcaactccc agggcaacga
    25741 aaaacaatcc ggccagatag atcacaatac gttttacaaa tttcatatac aaaccacctt
    25801 ataaaataca gggccccaaa ctgtacccgg actggcacag atgggtccct gtttgtcagt
    25861 tactatgtaa tatttcagga gagaattatc agcaataagt tttaatggtt tccagcaggc
    25921 tatttcatta acagtggata caaaaatccc agccatacga ccatcaaagc accgccaatt
    25981 cgtgtagaga tagaggcaaa tgccagaagg ttcatacggt cagcagctgt taaaacagcc
    26041 agatccccgc ttccaccgat gttacagcag caaagacctg cagttacacc tgcttcgtaa
    26101 gggaacagcc ccgccagttt ggcaaatatc atggcaccga taaaagcacc caggacgcca
    26161 aagaagataa cggcaaagac accagggctg aaaaacttgg tcaggtcggt gatctttaag
    26221 gaattgatgc caattcctgc aaccagcata ggcaggaggg cttttaaggc aaaattcatg
    26281 gagtaaacac aattatcagc tatatcatca gaaagaatac ccgtacattt aattacaatc
    26341 gcgataataa tggaccatgc caggccggcg atcacgctgg ttcccggaag tttgcctaag
    26401 atatcaccta agaggtagat caccagtgac ataaaaatgc cacttcccag ttttttgtag
    26461 tcggcactgg tgccggcccg tttttcttct tttaattctt ctgcgctctg gcttaccagt
    26521 atccttccgc caccgttcca gccgctcatt ttagcagtta cagcgccgcc tacagctgcc
    26581 atgagcactg caaggatatt ggcgatagaa gcgtagcaga ggaattgtcc tgccataccg
    26641 gtcatgtcct gtccggataa ggcgctgtac agagaaggaa gggtagtaat agcacctgcg
    26701 gaaccgccgg acatacaggg agcgccgata tagaaaagac cttcttttgc tccaaaacca
    26761 gtaatggcac cgccgatcat acagaaacca agagcaaaga cctggcttcc gatgatggtt
    26821 ggcagatagc gggcggttgc gcttaagatc agtttccggt ccattacaag aacagatccg
    26881 ataaggagca tggcaacgta catatcctgg aaaccgccct tcatgaccat gcggacacca
    26941 tttgctaaag gttcaggtac cagtcccaga taattcatca aagaggcacc aaggagtggc
    27001 agaagacagg ctccgcctaa atagttattt aaaataggaa tggtatttcc aagccagaaa
    27061 aagacaccgc ctactgccat aagaaaggct acagacccga caaatgtagt tgcagtaaag
    27121 gcacctgccg aattggaata aatggtcaca gtaggcataa atcccagata ggcactggca
    27181 aaaacaacgg cgaaaaacag tacaaaatgc ttgaatgaaa gtccgcatac atcaaaattg
    27241 atctttttac ggttggaatt gcgtttatta tgttcttcgc tgacggaaat accgaaacca
    27301 acatagtcta caccggcagc taccgcatca tctacaccac gcttatttaa agcaaggccg
    27361 atggtttgac atcccttttc ttttgcataa ggctgtacca gggcaaccag gtccttggca
    27421 tttgccattt gtggaacggc ttttggatta acgaaggagg taacctcgat cttatcagcg
    27481 ccggcatcga tcattttttt cacatatttt gcttttacct ctgttgggat gatcatagct
    27541 ttatggttct gccagccgtc tcttgggcct acttctgtaa tttcaattct ttttggtaat
    27601 gacatatggg ttctcctctc tgtattggat atacgatcca gttcctgtta tcattaaaag
    27661 caaaaatgat gccaaattga gaaaaataag agaaaacgtg attgttccag tatatgtggg
    27721 cgtaagatga atgggaaaaa tggtagaaat gcaaactttt atttacatca gtttacattt
    27781 gccggcgagc tgcagataga tatgacgtat tttctgccat ccagaagaaa tgttaaattg
    27841 acaggggttt tatttcaggt ataatatttt gtgaaacgat atacaaagat ggaaatgtcg
    27901 aagaaatttg gcaggataca aagaaatttt agcagcataa gcaaacacag gaggaacgat
    27961 catgcgcatc agtttggaac aggcattgga tatccttatg gatcatgtaa cacagggaag
    28021 aacagaaaga aaacctttgg aacaatgtct ggggctggta ctttcgcagg atgtatttgc
    28081 gttacttgat atgcctcctt tcagtcggtc tgcccaggat ggatatgctt tatgttctaa
    28141 ggacagcgtg ggggtagatg cagagaaccc ggttaagtta aaagttacag gaaagattta
    28201 tgcaggagat tatctggata tacaggtcag atcaggagag gcggtccgca ttatgaccgg
    28261 cgctatgata cctgccggtg ctgactgtgt ccttcgccag gaagatacag atgaagggga
    28321 aaatgtagta cagatctata aagaagtaga gccgggaggc agcatttgtt ttaaaggaga
    28381 agagtacaaa aaaggtcata ttctgctcca tgcaggtact aagatcgatg cggctgctct
    28441 ggcagttgcc agtgggaatg gtattatgga gctgcctgtg tatgaacggg tgagagcggc
    28501 agttgtttct tccggcagtg aagtagtaga gccgggaaca cctttgacgc ctggaaaaat
    28561 atataatacg aatactgtat acatgaaggc acgtttacat cagttgggcg ctcaggtgat
    28621 gatgagtcag actgtgggag atgatctgga ggttatgact ggggctttaa aagaagcagc
    28681 caaccaggca gaactggtga tcactaccgg tggtgtttcc gtgggacaga aagatctgac
    28741 agaagaggca ttgctttcca taggagcaaa gatcctcttt catggtattg ccatgaaacc
    28801 gggaatgcct acgcttgcag ctgaaaaaga tggtgttttg tttatagggc tttctggcaa
    28861 tccattttct gcagcaattc catttgaaat gtttgtacgt gagatcttgt cattaaaaat
    28921 gggtgatcca gagctgaagc tgcgtcggga aactcttact gctgtaaccg gtttctcaaa
    28981 gaacagcagg agacgtcgtt ttttaagagg aaaagcggtc ggaaaagagg tctggcttcc
    29041 agaccagcag gcaaatgggc agatgcgcag catggtaggt tgtaactgcc tgatcgagat
    29101 accggcaggc agtgggccgg tacaggctgg ggataaggta gaagtgctgt ggctgtaaag
    29161 aatgcggaca ttttaaatgt aggggcattt tttgctgtga tgaatacttg aaagaatagg
    29221 ggacgctact agacatatcc actttctttg gctataataa aggtaacttc ataaagcaga
    29281 tttcatatgt atatcccgga gataggtccg gagtttctac caactgccgt aaatagttga
    29341 ctacatagta catgtagctg tgagtacctg gctatgtgtt gctgtgtgcg ggtttggtga
    29401 cagatgaaag tgaattttaa aggagtcccg cgcaggcggg gctctttttt cattgccatg
    29461 catctggaaa ggagggcaaa tacgcacacc aaacgcagac gggctgtttt gtgggtttat
    29521 gatctgatgt ttacaaatcc tgattgagat caaggaggaa acatatggaa aataaaacat
    29581 ttgctttaaa gggaaatatt gtatacacaa aagataaaaa tgaattatgc atcaatgagc
    29641 agagttatct tgtctgtgaa aatggaaagg tccgggggat ttataaagag cttccggacg
    29701 agttgaaaaa tgtccctgta gaagatatgg gcgatcagct gatcatcccg ggacttacag
    29761 atcttcatgt tcatgctcct cagtattcct tccggggtct gggaatggat atggagcttc
    29821 ttgactggct ggagaccaat acgtttccag aggaagcgaa atacaaagat ctggaatatg
    29881 cgaaaaaagc atacggcatc tttgcagagc agatgagaaa aagcgccgct acaagggcat
    29941 gtatctttgc aacgatccat aaagaagcga ccattttgct tatggatctg atggaagaaa
    30001 gcggtctttc cactatgatc ggaaaggtaa atatggaccg caacagcccg gattatctgc
    30061 gggagccgtc agcgaaagac tcagaagaag cgaccagaga gtggcttaaa atggtggctg
    30121 acaaaaatta cgaccataca agaccgatcc ttacgccacg gtttacacca agctgtaccg
    30181 atgaactgat ggagcggtta aaaggaattc agaaggattt tgacctgccg atgcagtccc
    30241 acctgtctga aaatccggga gagatcgcct gggtgaagga attatgcccc tggtctgaat
    30301 tttacgggga tgcttatgac cattttggac tgtttggagg agagtgtcct acgatcatgg
    30361 cccactgcgt ttattccggt gaggaagaga ttgcccgcat gaaagaaaga ggtgtattta
    30421 tcgcccactg tcctgactcc aataccaatc tttcttccgg tgtagcaccg gtgcgccggt
    30481 atctggaaga gggaatgaac ataggactgg gaagtgatgt ggcaggcgga acaagtgaga
    30541 acctgtttgc agctatggca cagtccatcc aggcatccaa gcttcgctgg agattgttag
    30601 atgcttcctt aaagccactg accattgaag aagcatttta tatggcaacc aagggcggcg
    30661 gagctttctt tggaaaggtg ggaagctttg agcctggcta tgaatttgat gcagtggtct
    30721 gcgatgacag ccgtctggaa catccccagc cgcttactgt aaagcagagg ctggagcgta
    30781 tcatttatct gggggatgag agagaagttg ccggaaaata tgtagaaggc agaaagctgt
    30841 tttaatcatg cgaaagtaag ttgggttcag ctatttgcat cgtataaaaa gcagtcggat
    30901 acaggaaaat acagcctgtt ccggctgctt ttcttgtaaa ccacccggct tcatgttatt
    30961 atgtatacaa cgatctgttg cagagcaaca tgatcctgta tccgtgtaac aggcttgcag
    31021 atataaaagg agtagatcga tgatgtattg atgtatttga tccatttggg atatcttgga
    31081 tatgatcagg atgcgcagac agcatttatc ccaaatgaag aaatacgcca ggagctgaca
    31141 gcagcaacaa ggcgcaatcc caagaacatc agtgtatcat tgagaaatta taataaaaat
    31201 attgcagata aaactgttct gagcagcaga aaggacgaaa atacaatgaa atacgatttc
    31261 acaacaattt tagaccgaaa gggaaaagac tcgattgcag tggaaccttt tgaggctgac
    31321 tggatcaaat ggccggggaa gacgaaggaa ggttttgata ttatcccaat gtgggtggca
    31381 gatatgaact ttccggctgt acatacgatc ccggaagcga ttattgagag aacaaaacat
    31441 actacctatg gttacttttc tccaagagaa gaatattttg acgctatcat caagtggcat
    31501 aagaccagaa atggcataga gggactggaa ccaaagcaca ttggatatga aaatggtgtt
    31561 ttaggcggtg tggtcagtgc attaaatgta ctttgctcga aaggtgacag tgtactgctt
    31621 cattctccca cttatatcgg ttttacaaag agcctgacca acaacggtta tcacattgtc
    31681 cacagcccgt tagtgaagga tgagaataat gtatatcgca tggattttga ggatatggaa
    31741 aagaaaatcg tggagaacca tatccatgca gctattttct gcagcccgca caacccaacc
    31801 ggccgtgtat gggagcgctg ggagatcgag aaggccatgg agatctacaa gaaacatgat
    31861 gtatatgtag tttctgatga gatctggtct gaccttctct taaatggaca taaacacatt
    31921 acaacccagt ctgtatcgga agatgcaaag cagaggaccg tagcacttta tgctccgtct
    31981 aaaaccttta acctggctgg tctgattggc agttatcata ttatctacaa tgactggtta
    32041 agggaccgca ttttaaaaga gtcctcttta agccattata acagcatgaa cctgttatct
    32101 atgtatgcat taattggtgc ctataagctg gaaggttatg aatgggtgga tgaattaaaa
    32161 gaggtcttaa gtggaaatgt aaactatgcc tgcgattaca ttgaaaagca ttttgaagga
    32221 gtcaatgttt ccaagccgga aggcacctac atgcttttcc tggactgcac agaatggtgc
    32281 aaaaaacatg gaaagactat tacagagctt caggcagcag gcattgaagt aggcgtgatc
    32341 tggcaggacg gagtagcttt ccacggccca tgtcatatcc gtatgaacct ggctctgccg
    32401 ctgtccaggg taaaagaggc ctttgaccgc ctggacaaat atgtatttaa tctgcaggag
    32461 gcataagcaa catgatattt gatacacatg cccattatga cgatgaagcc tttgacgagg
    32521 acagggagca gcttttagcc agttttaaag aaaatggcat tgacgctgtt acaaatgtag
    32581 ctgccagtct ttccagctgc aagactacac tggagcttgc cagaaaccat gatttcatgt
    32641 atgctgcatt aggcgtacat ccaagtgaaa gcgcagaact gtctgatgaa ggcttaaaag
    32701 atattgagac ctggtgcaca gaaagacagg acggaaaagt ccgtgccatt ggtgagatcg
    32761 gccttgatta ttactgggaa gagccggata aagagatcca gaaaaaatgg tttgtggccc
    32821 agttagatct ggcccgcagg ttaaagcttc cggtgatcat ccacagccgt gatgctgcaa
    32881 aagataccct ggatattatg aaggcagaac acggatcgga catcggtggt gtgatccatt
    32941 gcttttctta tacaaaggaa atggcaaggg agtacctgga tatgggattt ttccttggca
    33001 ttggcggcgt acttactttt aaaaatgcca gaaaactggt agaaaccgta gaatatgccc
    33061 ccttagaccg cctggtactg gaaaccgact gcccatatct gtcacctgtt ccaaaccggg
    33121 gaaagagaaa ttcttccctg aacctgacct atgtggcaga tcagctggca gctcttaagg
    33181 gggtggaccg ggaaacagtg gagaaaatga cctgggataa tgccagaaag ctttaccgtt
    33241 tatagatgca gatctgcccg tcactggcgg gtggaagcga aatgatataa aaataataga
    33301 aacagaaaga agacaaaagc aggtaaaatc gtaaatgaat gagaaattat cagatccgaa
    33361 aaaaacaatt gaagtcatcc agaaatatca gtttgcgttc cagaagcgct tcggccagaa
    33421 tttcctgatc gatgctcatg ttctggaaaa aattgtaagc gcagcaggta ttacaaagga
    33481 tgactgcgtt ctggaaattg gtccgggaat tggtaccatg acccagtatc tggcagaaag
    33541 tgccggacag gtgatcgcag tggagatcga caccaatctg ctgcctatcc ttgcagatac
    33601 attaaaagac tattccaatg taaaggtcat caaccaggat atcctgaaag tggatatcaa
    33661 tgaactggta aaggaataca acaacggcag accgattaaa gtagttgcca atctgccata
    33721 ttacattact acccctatta tcatgggact gtttgaaagc aatgtgccaa tcgacaatat
    33781 cacggttatg gtccagaaag aagtggcaga ccgtatgcag gtgggaccgg gatctaagga
    33841 ttacggcgcc ctgtctctgg cagtgcagta ctacgcaagc ccatatattg tggcaaatgt
    33901 gccgccaaac tgctttatcc caagaccaaa tgtaggttcc gcggtgatcc gtctgacccg
    33961 ctatcaggag ccgccggtac aggtaaaaga tccgaagctg atgttcaagc tgattcgcgc
    34021 atcctttaac cagagaagaa aaacactgca aaatggttta aataactctc cggagatctc
    34081 attctccaaa gaagagatca caaaggccat tgagagcctg ggcgtatcac cttctgtccg
    34141 tggagaagct ttaagcctgg agcagtttgc ccagttagct aattacttcg tgcagtaagt
    34201 gggctgttta taaaaagaaa ggcaaatgcc tatgtcagat aaaaaaagat ggatccggct
    34261 ggctgtggct gcaggtttag gcgcggcagc cggtgcatgg acttatcaga tacaaaaaga
    34321 taaaaaagcg aaaaatgatg ccagactggc ggttgtggaa gcagctgtag tgcgcagccg
    34381 agattatgga aagcagaagg cttatattat aggaaatagt ttaagctctc tggcggcagc
    34441 agtctggctt ataaaagact gccattttcc tggaagcagt attatggtgt atggggaaaa
    34501 tgaggaggca tgtggaaatg caggtcctct tgtagtaagc cgggaaaact gcgaagattt
    34561 tctggaactg tgtggaaaac tgccaggttc cggggaaaaa gtgtttacgt ttccaaaaac
    34621 agaaggtgga gaacgtctgg gatgtgcaga ccttccggca ctctggcggc tgttatgtac
    34681 agaagaggaa agactggatg ttttaagtgt tgaagactgg ttcagtggga cacctcatat
    34741 atttgagaca gatctgtggc agctgtgcca gtgtgtgttt ggcctgaaaa aagacagcaa
    34801 tctggcggaa tttcgcagaa gtttatggga attaaaagga ccgtttctgt ttctttcacc
    34861 ggaagaaaga caggagctga tccgtttgct gaaacagtat ctgcgccgca aaggcgtatt
    34921 gttgctggaa aacactcagg taacggatct ggagcttgaa aatggaaatg acaacggaag
    34981 cggaaccggt ctggcagtga aaaaactgta tgtaaaacgc agactgcagg aagaagagac
    35041 tgaccgggaa gcttttgtat ttgaaaagat agatctgaga tccggggatg tctgcattat
    35101 ggataatggc agcggttctg gaaaattatg gaaaaaggca gctgacctgc atattgctct
    35161 gggagaaccg gaagcctttg tgggcgaaga agataaactt cagccccttc ggcctcatca
    35221 ttttacagac cggcctaaaa cagttccaac tggaagcaga aatcttggct ttgtgggagc
    35281 attttcacgg ctggaagaag gctgctgcct tacaggaaac tatgctgtgg caagtgccag
    35341 aagtgcagtg gatgagctga tgcatgcagg aagacgggca gcaggaacag aagaaaagag
    35401 gcagtcccgg atcagaaaat ggatcacagt ttacaaggtg gtttgttcat tgtaccgtgc
    35461 tgacttataa tttttgtgta tggtcatgcc gccatcaggc ggtctgttag tttccatgcg
    35521 tttgaaagaa actgccagtg acagtttcta taaagtattt gtttttaaaa ggaggacagc
    35581 aatatgctgt ttatcggagg tatcagtcag ggccggaagc tgcttaacta cgcaagaagc
    35641 attctttgcg gaagctgtgg cagcaccagc cagtgtcagg tctttatgac ctatatgtat
    35701 ttcagttttt tctttatccc gctgtttaag tggaataagc gcttttatgt gcagatgtcc
    35761 tgttgcagcg ccatttacga gctggagccg gagatcggaa aagccatatt aagaggcgaa
    35821 gaaccggcga tcacatccgc cgaccttcac ctggtccagg aaggaaaata cacccgcacc
    35881 tggcaggaag gttccggaaa tccccacaag aagtgtatgc gctgcggatt tgagacagac
    35941 gaagattata actactgtcc gaagtgcggg ggacggatat aaggaacgct taaggaaaac
    36001 aagcgccgct ttaagcggca tttgttttcc ttgcgtcctt aacgaacaaa ccgtctgcga
    36061 tagcagacag tagagcgcgc cagcgcgtgt ttgtgagtga acccaacgcc tgcaaaataa
    36121 atattaaatc tccccaaatg cgatcaatgg atctttgcat tttacaccca gactcagggt
    36181 gtaataaagt acaacgccat caaatggagc tgtacatcgg aaaatttcat tcccataaat
    36241 atctttcagg gtgccaagaa gggtgccctt ttttaatttc tgacctgcat gtgagacagc
    36301 aggataccag aacccatttt ttggtgcctc ttcataaact gcctgacgga tctcttcctg
    36361 gtgaaggtca gtatctgaaa aaggtctgat cttaagatga gccattaact caaaaatatt
    36421 ccttttgcat gctgtgacct catcttcgca ccatagccct ctttctcccc gttctgctaa
    36481 aagtgccggg ataccgcact gagcagccca gctatagagt ccgttttttg cagtagaagc
    36541 gacccgatat ggaatcgaga gtgaggctgc agctgcagaa gcttttgcgg ccagtgcgtc
    36601 agaaacagtt gtgggaaaga agaccagggg agtaagagct tcgttcacat caccaccatg
    36661 gagatctatg agaaaatcag cttcaggata cagtgtttct tcaaggagct ttgcaagacg
    36721 gctggaaaaa gtaccctcag gatctccggg aaacgtacgg tttaaatttt gaccatctgc
    36781 agggattatc tgtttggacc ccatgtaaaa gccttctgga tttaccaggg gaagcaggat
    36841 gatacgccct gaaagatcga tggggtccag ttcctttttc agacggttca gggtttctat
    36901 gcctacatat tcacagccgt ggacacctgc ggtcactaca agggtttttc cgggacagct
    36961 tccgcagata acagtcattt ctatgggata gtggctggca actggcagta aaagggtttg
    37021 ctttgtattg ctgccggctt ttaagtcaga cagtctcata tttttcagat ctcctttagt
    37081 tgaattcgat aatagaagcc aaaagctttc tggcatagtc atcttttgtt tctgcaatac
    37141 gggatacagg aatgtgttca gtaatgcggc catgatacag aaaaagcaca tcatcgcaca
    37201 gataagtgac agaagtcagg tcatgggtaa tgaaaatata agtcagcccc agtttttctt
    37261 tcagatcatg gagcagatcc atgatctgaa tctgagtatg ggcatccaga gaagagatag
    37321 cttcgtcaaa aagaatgatc tctggctggc aggctacagc ccgggcaatg catacacgct
    37381 gcaactgtcc gccggacagc tcatggggat accggtttgc ataggaggaa ttcagcccaa
    37441 caagctctaa caggcggttg atctcttcca aacggttcag gcggatcttt tgatttcgtt
    37501 ctcttgcagc cagtccttct gcaatgatct cttttacacg gaaacgggga ttggcagaag
    37561 tggtgtaatc ctggaaaaca acacttaagc gtttttgaag gtttttccgg ccatttctgg
    37621 aggcataaac agatacatcc tggatcttta cttctccggt gtcaggcttt aaaagaccac
    37681 agatcacacg gccaagggta gatttcccac ttccggattc tcccagtatt cccagacagg
    37741 ttccctgtga gacttttaag gacacatcaa acaacacctg gtttctggca gttccgaaaa
    37801 tcttttcctg gcgttcactt cggaagctga cgcatacatt atttacttca agcatgggat
    37861 atctcctttc catgtaagat gtcacggtag tggcacataa cagcagaacg cttttctatc
    37921 agcaggcggg tgtagggatc gtttgcatga tttcggatat gctcaaagtc tccgctgtct
    37981 acgatctgtc cgctgttcat aacaaggatg cggtctgcta ctttggagat ggctcccagg
    38041 tcatgggtga tgaaaagcat ggcagtattt ttattctttt taatgcggac aaactcttcc
    38101 aggatctcgt attgagtgac ggcatcaatg gcagttgttg gttcatcagc aatgagaagt
    38161 tccggctgca atgccatggc aataccgatc ataatacgct ggagcatacc gccggagagc
    38221 tgatgaggat atttttccag aacttcttct ccatttaaga tctgcatctg gtccaggatc
    38281 ttaaggcaac aggtacgtat ttcctgtgca ttccatgcag tatgtgcggc gaaggtttca
    38341 gccatctgat ttccaatgcg gtataaagga tcaaaacagg tcatagggtt ttgaagtacc
    38401 atggtgatcc ggcttccacg gagcctgcga agttcttctt ttgactcttt taaaagatca
    38461 tggccatcaa aaacagcgct tccggaaacc tggaaatttt tatctaacag acccaggatg
    38521 gattttacag tcatgctttt tccgcttccg gactccccaa ggatccccag gcattctcct
    38581 gggtggactt caaagctcac atcttctacc agctttttgg aggctttacg atatgtcaga
    38641 gttacattca ggttttcaac ttttaaaacg gcagacatta cagcacctcc tttgggtcta
    38701 acacatcacg gatggcatca cccattaagt taaaggcaga aaccagaata acgatggcaa
    38761 taccgggaac cagcatttgg gccggattac tggtcaggac attttttgct tcgcttaaca
    38821 tagcacccca ttcaggagta ggagcctgta caccaagtcc caggaaggac aaagtggaaa
    38881 tattgataat ggcccagcct acatccagag aagacaggac agccagatca gctgcaatag
    38941 atggaaccag gtgtctgaag aggataaaac gttccggagt tcctacacag cgggaaaact
    39001 ggacaaagtt ccggtcacgg tactgcataa cgcccgttcg gatcatacgg gcataccagg
    39061 cccatttgat aaatacattt gccaggatca cgttctgtac gttaatgccc agcagcgcaa
    39121 ctacagcaaa aaccatgatc tgactgggga aagacagcat tacatccact atacgcatga
    39181 tcagttcttc cgtaatgcca cgaaaatacc cggctaaaag gcccatcagg gcgccgagac
    39241 cgatggtgcc taacatggtc agcacagcca ggccaagagt gggacggatt ccgtagatca
    39301 tgcgggagag aatacagcgc ccaagattat ctgtgccaag gggatattct ttgctgaaat
    39361 gggcaaattt gttaagaata ttggtggcat agggatcatg tggagcaata aatggggcaa
    39421 aaaggcccat gaatgccacg atcagtacaa tggttattgt accaacggca gttttattat
    39481 gaagaaaccg gtgtaacatt agttgctctc ctttcgcata cgggggtcag acacggtctg
    39541 caggatgtca aacagcagat tgaacataac aaacaggaca ccgatgagca gaacgtaagt
    39601 ctggatcacg ggataatccc ggttgaaaat ggaactgatg cacaaggttc ccagtcccgg
    39661 ccatgcaaat acattttcca ctacaatggt tcctgagatc agctgtggga tactcatgcc
    39721 aatggcaaca atacaggtat gcatggagtt tttcaggata tgttttacca gaatggatct
    39781 ctgcgggaga ccgcgtacat tggcataaag tacataatcc tgtttcatat tttcaagcat
    39841 attgttgcgg atcaggcgga tataggtgga aatatagctt aaggatacag taaaagccgg
    39901 caggatcaga tgcctgatgg taccgtttcc gctggtaggc aaaaggtcca gtttaatgga
    39961 gatcagccac atcaacagaa gtccgaccca gtaagggggc atggcagtgg tcataaatac
    40021 aaggctccgc atgatccggt caaaccagcc atctttatat acagcacaaa gaaagccgat
    40081 agggagactt aaaaggatca ccataataag ggaagcacct gcaagctgta aagtagcagg
    40141 catacagcgc agcagttctc cggcaacagt acggcttggg tttacataac tgatgccaaa
    40201 atcaccgtga agacagtcca atacccaggt aaagtaacgg accagataag gcttgtccag
    40261 cccaagctgg gaacggacct cagcaatggc atcttccgtg atgataggta cctggcgtac
    40321 acgtaaagct acttcagccg gatcagaagg gatcaggttg ataaaaacga agcatacaaa
    40381 ggaaatgatg agaaggagcg gtatggcaga aaggatccgc ttgataatat agggtttcaa
    40441 aataatacct cccgttgaaa aaataaatgt aataaaagga tccgactgga acaaagccgc
    40501 ctcagaggag acggctttat acacaagatt aggtttgttt tgccagattt agaaagagaa
    40561 atcctggaat ggaacctcgt actgagtctg ggtaaagtgg aagcctttca tatcagagcg
    40621 gtagattgcc ttattgcact cataggtcag tggaatataa acggcatctt cgtggagatg
    40681 ggtcagaaca taggtataaa gttcctggcg tctgttttca tctgtggtga tcaggatctc
    40741 ggtaatggca tcatcgattt ctgctttatc atccagacca agctgcgctg catagtcacc
    40801 atatacagga gcgcgcatgg cagcaagaga ggactgtgga tcgtatggtg taccccagca
    40861 gatattgaat accatatcaa aattacctgc tttcatatta tcacggtagg actgctcctc
    40921 ttccccgtgg atgtttaaag aaacaccgat ggactggtat tcgctctgaa gatactctgc
    40981 aatggatttt tcagtcacgc tgtcactatt atagagaaga tcaatattta acggctgtcc
    41041 atccttttca cggacattgc cggaacccat tttccagcct gcttcatcca aaatctgtcc
    41101 tgccagttcc aggtcgtaaa catatggttc tagttcaata tcacaatatg gaacagtgga
    41161 tgcaaacaga gtgtctgccg gtttttccag tccgtaaaaa ataccgtcag agatattctg
    41221 cttattagtt gcatgctgga gcgcaatacg tacatccttc tctgaaagaa tgtcagagga
    41281 agtattcaga acaatctgtc tggtagaggt tggatcagaa agttccacgg taaactgatc
    41341 gctgtccaga tactgattaa tggcatctgc gtcgatcata ttttttccga agatcatatc
    41401 aatctcacct ttttccagag ccaggatacg ggtctgatta tccgggatga ctttcacggt
    41461 gatggttttg atggcaggct gttcgcccca gtagttcgga tttgccttga atacagcata
    41521 ctcatcggta acaaattcat ccagcacata gggaccggta ccaatataag cttctacccc
    41581 atctttggtg ctgccgtttt tcatggcttt cggggagatc atggcaaatg gtcttgttac
    41641 accaagctct gttaaaaggg gataatatgg ttcggataat tcaatcacaa aagtgttttc
    41701 atcgggagca tctacaccta ccaacagatg catcatttcc agccaggtat ggcggtcttt
    41761 attttccagg attgcgtcaa agtttgcttt gatggcatat gcgtcacact tttcgccatc
    41821 agaaaatgtt acgcctggac gaatgtggaa tgtatatact ttaccgtcat cactgacatc
    41881 ccagctttct gccagacagc cttcaaaacc gtcagaggta atattgacca ggccctcata
    41941 taacatttcc tgggcgtaca tttcgccggc atataaatgt gggtttaaat cgcggatatc
    42001 gcggtagttt acgaagatca ggtcttcctt tgtaccttca gctgtggtgg tagtttctga
    42061 aggattggac tctgcagcag aagatgcaga gtcagaagcg gatgaaccgc agccgcttaa
    42121 taaggcggtg cctaagagac ctgccagaag cattgtgccc catctttttt tcatgataag
    42181 tttcctcctg ttgtaaaaag aacataatgt tgtttattca ttcgcctgag gcgaacggta
    42241 gaaagtataa catgggttag gcaaaactta ctactaatga aattggaggg gcttagttat
    42301 tccaaaatgt tataaccggg cgaaaaaact tataaaattt ccttaaaatg acagataaac
    42361 cattcagcag ctttacttaa ttttgcatga tccgggcaga tgtagccgat gaggagaaaa
    42421 ggctcacaat tattgatcct aagctggcga acatcatatt ggcggtactg gtcatagaga
    42481 aaattaatgc cgaggctgca tatatctgtg ttcagtaaca gattgacagt cagatgatca
    42541 ctgttggtat agactgcata gtggagctgc tctgtgctga tcgccccaag gctcacatgg
    42601 ttcaaatggt gctctacaga gaaaaaatcc cttacacccc gtacaaagga aagagaagaa
    42661 aggtcctgaa agtcaatact ttcatcatga tagcggggat gctttggacc aacgtaaaca
    42721 caggcttctt tgctgtcaag aggaacgaat ttcagctttt tgtgggacag gatatgctgg
    42781 aactggtgga gctgtttctg tgcaatataa acgatgccaa tttcagaaag tccctgggct
    42841 acccggttgc tgatctcttc caccgtgcct tctaaatgtt ctacaatgta ggaatttccc
    42901 atttctttat aaaaatcagt caacagacgg gagatcatat tgctgggata agtggccaga
    42961 ctgaattttt ttccctggtt gtgatcggtc agtgagttga tcaggcttat attgtgcagg
    43021 atattctggg catattcacg gaccgtttca ccataggggg taatgtggat cccccggctg
    43081 ctgcgctcaa aaagaggccg tcccagttct ttttccaggg aactgatcac tttactcaca
    43141 ttgggctgtg tggtataaag gcactctgca gcctggttaa agctgccttt ttcacatgtg
    43201 atcataaaat attccagctg acgtaattcc ataagatcct cctgaagtac aataaacagt
    43261 aagttaatgc taactactat aacagaaaaa aagacagtat acaacaaggc tgataaagca
    43321 acagccgaaa gaagcactta ttgagaaaaa atgccggtta ttccaaaggg gaatatcaat
    43381 gtatccggcg ccataaagtg gtgtgttttg ataaacaact tgacaatttt taccaataaa
    43441 tgttattctg aatacgaaca gggagcttgt gaagcaggct gagaggaagt aatcgaactt
    43501 cgacctattg tacctgatgt gggtaatgcc aacgtaggga aataatgtgt cgcatattat
    43561 atttgcgtat cgtgcgtata ttttgcggtc cgttccttat gtgggcggac cgtttttttt
    43621 gtttctgcgc aaatccagat gatccacata tgcgctagag atctgtgcag caaatgtgga
    43681 aaacgttccg tccaggtacc ccttctgtgg aaaaatatta ttatgtgaag gagaaagaaa
    43741 atggactaca caacacagat ggatgctgcc agaaaaggaa ttattacaaa ggagcttcag
    43801 gcagtagcag aaaaagagca gatggataca gcccggttaa tgcagctggt ggcagaggga
    43861 aaagtagcaa ttcctgcaaa caaaaaccac aaatgcctgg atccaaacgg tatcggctcc
    43921 atgttaaaga ccaagatcaa tgtaaacctg ggaacttcca gagactgcaa agacctggat
    43981 atggagcttc agaaagtaaa tgacgcagta aaaatgggcg cagagtccat tatggactta
    44041 agctcctggg gagataccca gaaattcaga agaaaactga ctgctgaatg cccggctatc
    44101 attggtactg taccgatcta tgacgcagtg gtatactatc ataaggcact gaaagatatc
    44161 accaccgaag agtggttaaa gatcgtggaa atgcatgcac aggacggtgt ggattttatg
    44221 accatccaca ttggtatcaa ccgtgcaact gcccagcgtt tcaagaaaaa taaacgcctt
    44281 atgaacattg tatccagagg cggttccatt atctttgcat ggatggagat gaccggaaag
    44341 gaaaatccat actatgagca ttttgatgag atcctggata tctgccagaa atacgatgtg
    44401 accatcagtt taggtgacgc ctgccgtccg ggatgcttag aagatgccac cgatgtttcc
    44461 cagatcgaag agctggtaac attaggagag ctgacaagaa gagcctggga gaaaaatgta
    44521 caggtgatcg tagaaggacc gggccatatg cctcttaacc agatcgcagc caatatgcag
    44581 atccagcaga gcatctgcaa aggcgctcct ttctatgtat taggaccttt ggttacagac
    44641 attgcacctg gatatgacca tattacagca gctattggag gtgccatcgc agcaacctac
    44701 ggcgcagcct ttttatgcta tgtaacaccg gctgaacatt tacgcctgcc ggatctgaat
    44761 gacgtaaaag aaggcatcat cgcttccaag atcgcagccc atgcagcaga tattgcaaaa
    44821 ggtgttaagg gcgcttccga gtgggaccgc cagatgggaa tggcaagaaa gaaactggac
    44881 tgggaaaaga cctttgagct ttccattgat ccggaaaagg cgagaaacta cagagcatct
    44941 gcgaagccgg aaaaagaaga tacctgctct atgtgcggca acttctgtgc tgtaaaaaat
    45001 atgaaccgca ttttagacgg cgagatcgta agtatttttg atgaatgaaa tgagcccaaa
    45061 agaaaaacaa gcggctgcga agcagacatt tgtttttctt gggccattaa cgaacaaaca
    45121 gtctgcgata gcagacagta gagcgcgtta gcgcgggttt gtgagtggat gatggatatg
    45181 agaaaaggag agaaccatga aatttgacaa aagccagttg ttgatctatg cagtaaccga
    45241 ccgggcctgg accgggaaaa tgagtcttta tgagcagaca gaagcagcct taaaaggcgg
    45301 cgccaccatg gtgcagcttc gtgaaaaagg actgcgggat gacaacgtgg aagaatatct
    45361 ggaagaggcg aagaaaatgc gcgctctcac agaaaaatac aatgttcctc tcctgatcga
    45421 tgacaatata aaactggcaa aagcctgtgg tgcagacggt gtccatgtag gccagaatga
    45481 catggatgcc ggtcttgcaa gacaacttct tggaccggat aagatcctgg gagtcacagc
    45541 caaaacggta gaacaggcat taaaagccca gtcacagggg gctgactatt tgggcagcgg
    45601 tgccgtattt ggcacatcaa ccaaaaaaga tgcccgccct atgacgatgg aacagctgca
    45661 ggcaatctgc gcatctgtaa agatcccggt agtagccatt ggcggtatct gcctggataa
    45721 tattgaaaaa ctgcagggaa gcggtgcagc aggagctgcc attgtatccg gtatttttgc
    45781 agcagaagat attgaaggga ctacccgagc ccttcgtcaa aaaatggaac gtattattta
    45841 gacgatgcca agtggttaca actgggatgt ccgtcataaa aagacatgat atttcaaaaa
    45901 atgagaaaaa ttcttgataa caaaggggac tgctataagc ggtccctttt ctatttgtcc
    45961 tgtttttaac aaattcagaa aaatccttta tttaagccaa ttctgcgtta ttctcaccaa
    46021 actataacaa taaactgata acaacttttt ttaatggaaa tttttccaat aacctgcacg
    46081 atttcttgtt gaaaaatttt atcaaaataa aactgcccct cataattgac gaaaaaattt
    46141 cagttagtgt atactaatgc ggcatgcgag ttagtatgca ttaactcaca tgttcagtaa
    46201 tctacaaaag aggagttttg tacttatgag aaaaacagga agaacaaaag cactgttagc
    46261 catgtttctt gcagctgcat ccttgcaggc aggaactgct tttggtgcac aggcagcaac
    46321 cgggtcacag ctgcaggcgg gaacctgggt aaaagaagat gcaggctgga aatatagaaa
    46381 gccttcagga gaaatggcaa ctggatgggt ccatactgca agcggctggt attatctgca
    46441 gcctgagaca ggtattatgg caaccggcat gctccgtatt aatgaccgta cttattattt
    46501 taatcagaca ccagatgctg tagaaggcaa aatggttacc ggatggtacc aggatgcagc
    46561 tggcaaatgg tatttcttca gcactactgc agacagcacc gaaggtgcag cagtaaccgg
    46621 atggcagtgg gtcgatggcc gcagctatta ttttgagccg gcagcaggag agaacactgg
    46681 aaaaatgttt gcaaatggtg taacaccaga tggctacaaa gtcaatgcag acggacagtg
    46741 ggtagatgaa aatggaaaca ttcaggtagt gcctggaaaa ggattttcaa gtaatcctgg
    46801 aaaagatacc aattctaaga ccactgtaag aggcggaggt ggcggtggaa gcagccacag
    46861 cagctccggc agtacagata acggaaataa taatgccaat aacggcaata caaataatgg
    46921 aaacaccaac aacggaaaca ccaacaacgg aaacaccaac aacggaaaca ccaacaacgg
    46981 aaacaccaac aacggaaaca ctaacaatgg aaataccaac gacagtaatt ccagcaacga
    47041 caatacaaac aatggcaaca attccaacaa tggaaataac cagccagacc agaataaagc
    47101 agtttttgta aagcaggatc agacaaaact gacagagtta aattcccttg gctggtggat
    47161 tacggtagca tttgaagatg gctataatgc aggcaataca aaggtctatg cagatggcgt
    47221 agatgtaact gcagctcttt caaatgtaac tgatgatgga agtatatgca aactggctgt
    47281 tacaaaggca ccggctgttc ttactgtaag cagcaatgat gacggggcac aggtacagac
    47341 cgtttcctta gatgatactg cagcagatgg tattatctac acaggagaca gctatcttcc
    47401 tgaaaagatc atgacacacg gaccggtatc catctgggat tactatctga ccaactatgc
    47461 agatgatggt tctgtaagat attcaccatc taagacaaca ttcgctcttg gagaaaagcc
    47521 ggtggctcat cctgcttatt caccggatgc agaattaagc gaagatggaa aagcaactgt
    47581 aaccattatg ttcaattaca ataccgacga tgagaaagag tggtttgacg gaattaaccg
    47641 ccttcagctg gtagagtaca atgaaaataa gaacaccatc aacagtgatc ttgtatttga
    47701 ccgtgcaaaa gatgtagctc atggaagtgg ccgcgtaggc gaactgacca ttaagagcgg
    47761 acagggcaat ttcacaaaca acggaagata ttatgtccgc gtagagtcca aggcaggtac
    47821 atctgctctt gttccgatcc atgtagtaaa tgcccaggca cctgttctga aattacagga
    47881 aacagcccag tctggcgtaa acctacattt ccaggttgaa aacatggtat acggaattga
    47941 gacaccgatc agccgtgtgg ttctggaagg accaactgag acggtagaat taaacgagat
    48001 cgatgactgg ttcctgttca gccaggatct gtttgtcctt tataatgata atgtagatca
    48061 tttaaaatat aagggcaatt acaagctgac tgtatatgca gacggattta aggcaacctc
    48121 tgtcagattt aaagtaacaa atggtgaaaa tgttccattc aaggatgaaa tgggaatttc
    48181 ccagtattca tttgatggcg tctccacagc atccacaggt tcaggcggag gcagcagctc
    48241 cggcgaaagc agttccggaa gcagcgctat ttctgcaaat ctggtatttg ataccgacct
    48301 gctggtaaat gcagaacttc tggaagaagc tggaagatct acagctgagt ctgaggcagt
    48361 tgtagactgg tggtacaaca ttatcaagga tgcagtattt gacagagcag aaggaaaata
    48421 ctatgactgg accgattatg tatccagcgt agaaaataag aaatacgaag acgatcagat
    48481 cctttccttt gaggattaca agagaaccgg tgtgacctgg agcaacagtc cggcaactgc
    48541 aaaggaagtt ctggaagatg gtcttttagg tgagatccag aataacggtg attttgcccg
    48601 cccggcagct ggtgcattca cagtagagca gaacaaggaa ggcgaaaatg ttgttctgaa
    48661 gcatgaaaat gcacagtatc tggctgctat caaaaatgtt gtgatcaatg acagctggac
    48721 cggacttgca aaagacaagt atacagtaga tgttgaaaag ggaactttaa ctctggatca
    48781 gagcctgttc acaccgggag aagaatacaa gatcgcagta gaagcagacg gataccagat
    48841 gaatgcattt aagattactt atgatcagct tctggaagag ggactgactc tgactgtgaa
    48901 gtatccagat ggcaaaaatg agtatgcatc tgataagaga acctttggca agatccaggg
    48961 atactatgca gacgcagaag tagatgtaac cggttctgaa ggcggtttct taaagtatct
    49021 gacctctgta gttttagatg aggatgatac cctttataca aagggctatg aaggaagcga
    49081 tgcagcatat tatgagatat ctgctgacaa gaagagcctg actatcggaa acatcaaacc
    49141 tggaacacat acactgactc ttcgcgcaaa gggatatgta gatgcgttaa cagcacagat
    49201 cgtagttgca gaaggtgttg acgagcctga ggttactttt gttccggttg taaagagcct
    49261 ggagtacaag gaagctggct tcttcatgtc tcctgccaac tattacatta ctttcgagaa
    49321 tgatacagat actgatgcta agaaggtgct ggcagattat cttgacgcag taaaatctgt
    49381 tacggttggt gatgtgactt atacaaaggc attacttgga cttggatata ataataatga
    49441 gttcgcagct tctgttgtag atagtggtta cggtagcatt aatgactgcc tgcgtatgac
    49501 tgttgatact ggatttacag gtgatacagt tgaaattgtt gttgaggca
//
LOCUS       DBMDUA010000003        42919 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_783_length_42919_cov_3.837055, whole genome shotgun sequence.
ACCESSION   DBMDUA010000003 DBMDUA010000000
VERSION     DBMDUA010000003.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 42919)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 42919)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42919
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_783_length_42919_cov_3.837055"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="ACZPCL_00430"
     CDS             complement(<1..188)
                     /locus_tag="ACZPCL_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1160994.1"
                     /translation="MGKMTIQQILMQLAGGMGTSIQIFAVTLIFSLPLGLFVALGRMS
                     KNKLLQAVIKVYISIMRGT"
     gene            complement(342..1217)
                     /locus_tag="ACZPCL_00435"
     CDS             complement(342..1217)
                     /locus_tag="ACZPCL_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1160995.1"
                     /translation="MKKRVLCAALASMMALSMTACSSGSTATTAAETKASEAETTTEA
                     ASSEAEETTAAAAGIEGGTLIVGFDQDFPPMGFLGDNNEYTGFDLELAQEVAKRLGLE
                     YQAQPIAWDAKDMELESGNIDCIWNGFTMTGREDDYTWTEPYMENTQVFVVRNDSGIE
                     GKDGLAGKVVECQADSSAEAALKEDPDLTATFGQLLTTADYNTAFMDLQQGSVDAIAM
                     DVIVAGYQIQQRKADFKILDDSLSAEEYGVGFKKGNTELRDKVQAALEDMAKDGTLKE
                     ISEKWFGEDVTTIGK"
     gene            complement(1509..2786)
                     /locus_tag="ACZPCL_00440"
     CDS             complement(1509..2786)
                     /locus_tag="ACZPCL_00440"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR1160996.1"
                     /translation="MLIKNGRVIDPAGKSERNADIVVKDGKIEKIYSEGILPAEAEAA
                     ADQVIDAKGLVVAPGLVDVHVHFRDPGLTYKEDIHTGAAAAAKGGFTTVVCMANTKPI
                     VDNEETLRYVLEEGKKTGIHVLSCAAISKGFEGKERTDMEGLLKAGAAGFTDDGIPLM
                     DGPFVKEAMEEAARLDTVLSFHEEDKTFIKQNGINHGKVSDQLGIYGSPALAEDSLVA
                     RDCMIALATGAKIDIQHISSGHSVEMVRLAKKMGAHVWAEVTPHHFTLTEDAMLEHGT
                     LAKMNPPLRTEWDRQMLIEGLKDGTIDMIATDHAPHSKEEKEKPLTEAPSGITGLETS
                     LALGITSLVKPGHLSLTELMEKMSLNPATLYKLDCGRMEEGAPADFVIFDENEKWVVD
                     SFASKATNSPFLGAELTGRVRYTICDGKVVYTL"
     gene            complement(2859..3575)
                     /locus_tag="ACZPCL_00445"
     CDS             complement(2859..3575)
                     /locus_tag="ACZPCL_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1160997.1"
                     /translation="MKVTYIHHSSFLVETDENYFLFDYFEGELPEFKEDKPLLVFASH
                     RHGDHFSPVIFELAGKHKKTGFVLSDDIWKRRVPEEFLGKTYFTGPGEELELTAPFTG
                     GSGVKIRAFKSTDEGVAFLIETEGKRIYHAGDLNNWVWAGEPEADNKRMSENFHKELE
                     KMKGIHIDVAFMLLDPRQEKDFYLGMDDFMKMVGADVVFPMHFWGDFEVTDRFKALEC
                     AKDYKNRIVKIHKKGESFLI"
     gene            complement(3618..4478)
                     /locus_tag="ACZPCL_00450"
     CDS             complement(3618..4478)
                     /locus_tag="ACZPCL_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1160998.1"
                     /translation="MNSKTKGILCIVTAAFCFAFMSLCVRLAGDLTSMQKILFRNLLA
                     FFIVGAGLVRKPEKLEIHKGDLKFLFLRSICGTVGIVCNFYAIDHLVLSDANMLNKLS
                     PFFTIIFSYFLLKERLTLFQGIAVVVAFMGALFIIKPTGNMTDPAALIGMLGGAGAGA
                     AYSFVRVLGKRGVNGKLIVMFFSAFTCVFALPFLIGHFAPMKWWQVAAMIAAGFGGAG
                     GQIFVTKAYFYAPAKEISVFDYSQIMFSALMGFLVLGQKPDMLSLCGYVIICTTAVCM
                     YFYNKNRRTL"
     gene            complement(4500..5873)
                     /locus_tag="ACZPCL_00455"
     CDS             complement(4500..5873)
                     /locus_tag="ACZPCL_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1160999.1"
                     /translation="MMMQKEKTVGKAKTVGTLFSGNDLKKLIFPLIIEQILAVSVGMV
                     DTMMVSSAGEAATSGVSLVDIINVLIINIFAAVATGGAVVASQYLGQKRKDRACQSAD
                     QLILVTGVVALVIMTLSILFRWQILNLIYKGVTEEVMKNAVIYLTISAISYPFLAVYN
                     SCAALFRSMGNSKISMQASIIMNVINVIGDALFIFGFHWSVAGAAGASLISRMTACFI
                     LMWRLRDPKLDIRIGTTKGVNMRMVKQILHIGIPNGIENSIFQLGRVLTVSIIAMFGT
                     TQIAANAIANNLDSMGVLPGTAMSLAIITVIGRCVGAGDFEQAEYYAKKMMKLTYVIN
                     GICCLVVILTMPVTLRLYGVSGETRHLGAVLALIHNGCAIFLWPAGFCLPNVMRAAND
                     VKFPMCVSIASMFIFRIGLSYVTGVAMGLGAIGVWIAMIIDWIVRISFFVTRFFSGKW
                     KTFYHGE"
     gene            complement(5870..7381)
                     /locus_tag="ACZPCL_00460"
     CDS             complement(5870..7381)
                     /locus_tag="ACZPCL_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1161000.1"
                     /translation="MNIKRAKEEIKDTIAAYLLKDEFGEYKIPAIRQRPVLLIGPPGV
                     GKTQIMEQIARECEIGLVAYTITHHTRQSAVGLPFIEHKIYGGKEYSVTEYTMSEIIA
                     SVYDRIRETGLKEGILFIDEINCVSETLAPTMLQFLQCKTFGSHKVPEGWIIVAAGNP
                     PEYNKSVREFDVVTLDRIKKIIIEPDLNVWKEYAYAQNIHPAIMSYLNIRPQYFCTVE
                     TTVDGKRFATPRGWEDLSQLIQVYETLGKKTDRDVVGQYLQHPKIAKDFANYLELYYK
                     YQDQYQVEEILSGTIREEVCDKLQKAPFDERMAVTGLLLAKLTDGFKELKRMNETMTL
                     LMAQLKQFKKELDGADVHALAPAMILESIGAELESIRIHKKESGLSDRRQDHIYRKVK
                     ELLEQYVQQMKARSLQEKEDSWNWLRQQFMEKSDAYEDKKTVCGERLEHAFDFMEAAF
                     ANGQELVIFVTGLNTGEASVEFLKEYNCERYYRYNKELLFDNQENEMKEVLGK"
     gene            complement(7429..8802)
                     /locus_tag="ACZPCL_00465"
     CDS             complement(7429..8802)
                     /locus_tag="ACZPCL_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR1161001.1"
                     /translation="MENPTRQRQLEELDQVELCTRILYQARNELYVNMHFLDVSLSSL
                     GFEADWNRNGMACDGAVIYYGPGFLLGLYKKGRAVVNRYYLHTLFHCLFCHLYTRKGR
                     EKKMWDLACDIAMESVLDGMYEKCIHVPQSPLRREMYLRLRRFLTGNKNTGASNEEER
                     KVVLTAERVYHALMEMELPKRRMEQLEAEFHVDDHDLWEQEPDPSAAMTRQNQWNDNR
                     ERMQTQMETMGSEEESENEQSLLDSIQVENEERYDYRQFLKKFAVLREEMQTDPDSFD
                     QAFYTYGLSLYGNMPLIEPLETREVQRIQQFVIVIDTSMSCSGELVQRFLEETYDVLS
                     QSETYFRKVQIHVIQCDDKVQSDVILTCADDLKAYMEHLQIKGYGGTDFRPAFEYVNT
                     LKVTGQLNGLKGLIYFTDGKGIYPVAAPTYDVAFVFIKERFSEEDFPAWAMRVVLDEE
                     TFNERRQ"
     gene            complement(8802..9209)
                     /locus_tag="ACZPCL_00470"
     CDS             complement(8802..9209)
                     /locus_tag="ACZPCL_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HIR1161002.1"
                     /translation="MELVLYYSTGDARMKKQEMMMKSVLVRMGVKIRNVAPDQVLESV
                     GYLAGVPGFEKREVSETLETEEKLPEITEPMLVMRDFTGRRIDTLLLNLRKAKVPKIN
                     LKAIVTEQNAGWSFYHLYEEIGEEHRLMNGGAQ"
     gene            complement(9237..9689)
                     /gene="dtd"
                     /locus_tag="ACZPCL_00475"
     CDS             complement(9237..9689)
                     /gene="dtd"
                     /locus_tag="ACZPCL_00475"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR1161003.1"
                     /translation="MRAVLQRVTRASVTVDGEVLGKIGKGFMILLGVENSDTEEITDK
                     MADKICKLRIFEDENGKTNLSLADVGGELLVISQFTLYADCKKGNRPSFVKAGAPDMA
                     EHLYEHFMERCRNYVDVVEKGQFGADMKVELLNDGPFTLMLESEQLFK"
     gene            complement(9702..11507)
                     /locus_tag="ACZPCL_00480"
     CDS             complement(9702..11507)
                     /locus_tag="ACZPCL_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR1161004.1"
                     /translation="MKLFLKLTDNVIKQNLRQLVLFTFLYRLVAGIFYVKTVNGILRF
                     SLHMAGYSYLTIGNLRAFLLHPFTIPFVIFILLLGMIFLLIETGAMITAYHSSIYLRK
                     ISAVSIFLGGLSKAKNELCRKNGKLLLAALGNYILMNCYFLVRILTRMKPVNFVLYEI
                     LHAAGTRMALVVGCVLLTVFSVPAMMVFFACMLEQKNFRDGVRESREILKGKWPRAVL
                     LLVVLNLFLILGLVLLYGAVMVIAAILVTLFAKAYTATAVMATVSYRIEWMILFLGSV
                     MAVVTDFGAMTVLYHQFGFTVPVPAKEYDFPKNREGYSRKRWFLTLTAVFSGIAAFMV
                     FDMVYNGNSPDQAILSQVEITAHRGSSKHAPENTMAAIEEAIGEMADYSEIDVQLSKD
                     GVPVVCHDLNLSRVAGVDVSLKNLTFDELEQLDVGSHFSSRFEGEKIPSLEEVLEACK
                     GRIRLNIELKSIGTKSPLPEVVTEMIKEMGMEDQCVITSVSLDYLKRVKEADPELKTG
                     YILAAAYGSYYKNEFVDFISIRSSLVTKKQIQAAHEYGKAIHVWTVNSRSEIEQMALM
                     GVDNIITDYPTRAKEVLYQPDVAKNLLTYIRQVMK"
     gene            complement(11510..12172)
                     /locus_tag="ACZPCL_00485"
     CDS             complement(11510..12172)
                     /locus_tag="ACZPCL_00485"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIR1161005.1"
                     /translation="MSVIPKTKDQVDQWRSVMFLYDSALKKLNTKIEILNNEFVNRYD
                     HNPIEHIKSRLKTADSIVKKLKKDGYEVTIENMMDHLSDIAGIRIICSFTSDIYQIAD
                     MIAAQGDITVLHVKDYIQNPKPNGYKSYHMVVTVPVYLADGPVQTKVEIQIRSVAMDF
                     WASLEHKIAYKFEGNAPEGLLRELKECADVVDMLDKKMFSLNEAITAYAKEHPETDPN
                     RE"
     gene            complement(12185..12949)
                     /locus_tag="ACZPCL_00490"
     CDS             complement(12185..12949)
                     /locus_tag="ACZPCL_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR1161006.1"
                     /translation="MNEEMNKQQEAEVKAEAPVETMEDYAAELEASYKAFDERHFELP
                     ETDDAEGEKWAEFAQMMADRTVLNVKIAEAVKGGVVAFVNEVRAFIPASQLSTEYVEK
                     LEDWQGKHVDAVIITVDPEKKRLVLSGREVEKEKKAAQRKERLAQFKAGDVVEGTVDN
                     IKDYGAFVKLADGIDGLLHISQISTQRIKHPGAVLKEGQTVKVKILSIADGKISLSMK
                     ALIEQETEEEEHETFDYKETAAATTGLGDLLKGLKL"
     gene            13504..14280
                     /locus_tag="ACZPCL_00495"
     CDS             13504..14280
                     /locus_tag="ACZPCL_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HIR1161007.1"
                     /translation="MKDILDLHTHTIASGHAYNTLYEMAQAAADKGLAVYGCSEHAPS
                     MPGTCHEFYFTNFKVLPRVIRGVPVLMGAELNILDFEGHVDLDEYYLKKIDYAVASLH
                     TPCIDNNGSVADYTRAYLNVLENPYVNIIGHPDDSRLPADWTEVAKAAARNHKLLEVN
                     NTSLNPLGARKGAKDNYKELLAACIRYGTSIIIDSDAHCEADVGNHSLAHAFLEEMHF
                     PEELIVNTSLEKAAAFIPKLKQLLSENEAFRASAERNTAL"
     gene            14277..15263
                     /locus_tag="ACZPCL_00500"
     CDS             14277..15263
                     /locus_tag="ACZPCL_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1161008.1"
                     /translation="MINFLNLEYFLVAAEELNFTRAAKRLYISQQSLSNHISNLEKEF
                     DVVLFNRTSPLTLTYAGQALKTRARELLDLRDETYKEISDIKDFSTGQLSIGVSHTRG
                     RFILPEILPTYQREFPGIELHLAEGNSSELANDLLHGNIDLMIDLLPFTAENVATVPI
                     CQEEILLVVPDEVIRKAYPDRFEEIKKSLYQSADVRLLENCPFVLLKKGNRVRTIADE
                     IFEDTQISPHIVLETENIETVLALSAQGMGITFYPRMFMSTRQNLNAYKDSLMGQAGL
                     NLFSLNYSRAHGTLAIGYHKGHYMSRATHEFIRIALEHIPSAAGLPSNSDAI"
     gene            15378..15797
                     /locus_tag="ACZPCL_00505"
     CDS             15378..15797
                     /locus_tag="ACZPCL_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR1161009.1"
                     /translation="MKTLKYSRQRESIKANLMSRHDHPTADALYASIREEFPNISLGT
                     VYRNLNLLVETGEILKLTCGNGPDHYDGNVTPHYHFVCRDCGQVYDMELDEMADLNTS
                     VQAAAPGKIDSHSILFYGRCYSCMEKEETENTLKKSS"
     gene            15979..16524
                     /locus_tag="ACZPCL_00510"
     CDS             15979..16524
                     /locus_tag="ACZPCL_00510"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HIR1161010.1"
                     /translation="MKKWVCTVCGYVYEGENPPEKCPQCGVPASKFKEQAADETSWAC
                     EHEVGVAQGSPEDIMADLRANFEGECSEVGMYLAMARVAHREGYPEIGLYWEKAAYEE
                     AEHAAKFAELLGEVVTPSTKKNLEMRVAAENGATAGKFDLAKRAKAANLDAIHDTVHE
                     MAKDEARHGRAFAGLLKRYFG"
     gene            complement(16615..17265)
                     /locus_tag="ACZPCL_00515"
     CDS             complement(16615..17265)
                     /locus_tag="ACZPCL_00515"
                     /inference="COORDINATES: protein motif:HMM:NF019349.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1161011.1"
                     /translation="MQKETLKERVYRGIYEAVTNGEYRPNDILTENQMIEKFGVSKSP
                     VREALVELCKDGVLINIPRMGYQVRAVTLKEIMDILEFRVDVEVGGLRKSFPVLTREN
                     INMLRMIAEKHNADEDRIVSNNWQRNYEFHTYLYSLNNNVYGHQALQQMIHQCSRYIS
                     QYFLSAWQRKNESNGRYHMAVVDALEKQNIEEACVYLEKDILAVKEEILALHSMNI"
     gene            complement(17348..18748)
                     /locus_tag="ACZPCL_00520"
     CDS             complement(17348..18748)
                     /locus_tag="ACZPCL_00520"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HIR1161012.1"
                     /translation="MEKEWIEKHIDDMVQDICKLSEIPSVSVKTEDPDMPFGQACLDA
                     LHAALDLGRSMGFETFNHENYCGTLLWKGESDTEIGFFGHADVVPAGNGWTCCEPFKP
                     VVKDGLLIGRGVSDNKGSFMTALYALKYLKEQGYKPKHSFRFFFGCDEEKGMADVQYY
                     AKNYKEPAFSIVPDVMFPVCNGEKGILEFDATRPVKSSKLVSFESGIMSNQVPAEAVA
                     VLTLTEEETSKVKEVVEAVGGTCEKQADGSVKVYVHGIPAHAAFPEGSESAEVKMAGL
                     LKKSGVLDEDAANLMDAICEMFGDYYGAGLNIPFEDEGSGKLTHVGGMCKLENGVFWQ
                     DVNIRYNVTAVYEVLMENITKTLKAHGFELTEAHDSAPCFTDPKSKEVQALMEVCNRN
                     LGMELEPYVMGGGTYSRKLKHAVGFGPGIPGKAKRFGTERGGAHQADEYIEIEHLKKA
                     FVIYVEAIQKLDEVVD"
     gene            complement(18818..19798)
                     /locus_tag="ACZPCL_00525"
     CDS             complement(18818..19798)
                     /locus_tag="ACZPCL_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1161013.1"
                     /translation="MKDNVILEVKNVEKFFYGNKKLFQKDRPVVKAVNDVSFTINEGE
                     TFGIVGESGCGKSTLGRTVIRLLNPTSGQVLYKGTDLATLSGEEMRKLRQEIQIIFQD
                     PYASLNPRMTVRELIQAPLDVYSHESEAEKLAKVKNIMAKVGLREEYMSKYPHEFSGG
                     QRQRIVIARALILEPKFIFCDEPVSALDVSVRAQVLNLMKDLQKEFNLTYMFISHDLS
                     VVRFLCDRIGVMYLGRMVEIADRQELYDNPMHPYTKALLSAIPVPDVDVKHEPMLLEG
                     DIPSPYNPPKGCLFHTRCKFAMECCKTEVPQMRQIGENHCVACHLYDDVK"
     gene            complement(19788..20777)
                     /locus_tag="ACZPCL_00530"
     CDS             complement(19788..20777)
                     /locus_tag="ACZPCL_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1161014.1"
                     /translation="MEPLLEVKNLKTSFKIGKKWVPAVEQVCFHVDPGETLGIVGESG
                     CGKSVTSMSIMRLLPKATTKIEADEIILNGKNIANYSDKEMAQVRGTGIGMIFQDSMT
                     SLNPVKTIGKQLDEAFIVHHSCDKNEAKNRSIEMLTKVGIPSPEKRYGEYPHQLSGGM
                     RQRVMIAMALAQNPSLIIADEPTTALDVTIQAQIMELMKEMKEKTGVSIMLITHDMGV
                     VAEMADKIMVMYAGMVIEYATAREIFKDPKHPYTKGLLASIPRKDKDIDRLYTIEGTV
                     PSLTSMPKGCRFCDRCTCAMEKCRNEQPPMYQFGERSVRCFLYEDKGGVSDER"
     gene            complement(20931..22595)
                     /locus_tag="ACZPCL_00535"
     CDS             complement(20931..22595)
                     /locus_tag="ACZPCL_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007036114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1161015.1"
                     /translation="MKKSNLLKKAVSVLLVASALAGCGSSASQTATTAAPAAAGTETS
                     EAATEAASSESSAEPGTITMAMVSAWDSIIPFDTTSSYSDAVLDLIYDKLVYLKQDGT
                     YEPRLADKWSMSDDNKVLTVHLNENAKWQDGEPVTADDVVFTMQVYNSPSAAVVRQNN
                     VSPFAGFKEGDDSLQVKAVDEHTVEMTCAEPTNMDFLFFIKFRDIYILPKHLLGDTPY
                     ADIRNNSYWEKPIGSGPCIYESQISGERMEFKANKDYYLKTPQWDRFVVKVVATPSLL
                     SGLMNDEIDILAGNVASLQLSDWDMAQQQQNLTCVSTESVGYQYMAINTEKDYLPPKV
                     RQAIDMAINRDAIVSGLLKGEGEPAFGPFATSHKYYDDVVNVGYNPEKAKELLAEAGW
                     DGSHELVFSVPTGNSIREQAAVIIQQNLQEIGIKTKIESADFATHLTKVREGNYDLGL
                     IGSGGSPDPSECVINFKPDHINNFSHLSDWTIYNTGAEGEHAFSFEDRKVNYDNYQEL
                     LVEQVPFCFLYFQNNLFAYNNRIGNVKDNQDYSQLNRDVWNWTIEK"
     gene            complement(22652..23518)
                     /locus_tag="ACZPCL_00540"
     CDS             complement(22652..23518)
                     /locus_tag="ACZPCL_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161016.1"
                     /translation="MAKENNRSSYFGDVVKKFLAHRLAMMGTIVLILEVLAVVLLPSI
                     MKLDPYSMDYMAFGGAPSAEHLLGCDDVGRDLFARLIFGGRVSLMVGICSALISLAIG
                     VPLGLFAGYFRGAVETVIMRCADIFMSFPNMILILVLASVLGQSVTTVTLVIGFLGWT
                     EFARLIYASVLSVREKDYVESAKAIGAKNGTILLRYVLPNSFAPVIISFTFRTAQAII
                     TESSLSFLGLGVQPPEASWGNILYYAQSISVLSTKLYMWVPAGVVLVLTVLSINFIGD
                     GIRDALDTKMKI"
     gene            complement(23535..24494)
                     /locus_tag="ACZPCL_00545"
     CDS             complement(23535..24494)
                     /locus_tag="ACZPCL_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161017.1"
                     /translation="MQMLRYIGKRILIAIPTFLGITVLVYVLSSMAPGTPLEMIFNDP
                     NATAEQMAAMKAKLGLDQPVMIQYFNWLVQLLHGNMGASYRTGHAVWADISERIGPTL
                     LLTMSSLVFSILIAIPLGIMSAYKPYTAWDYISSAFSFIGAAIPNFFAGLVFVYLLSV
                     VLKIFPSGGMYDTSGVRTLPMLLHHLFLPMLVLSIQQVGSLIRQTRGAMLDVLQEDYI
                     RTARAKGALEGRVLFKHALQNAFIPIITRIGMMIPFLVGGAVVTEQIFAWPGLGSLMV
                     LSINSRDYPVIMGITVFIAAAVLIGNMIVDLVYGLLDPKIRYS"
     gene            complement(24519..25496)
                     /locus_tag="ACZPCL_00550"
     CDS             complement(24519..25496)
                     /locus_tag="ACZPCL_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR1161018.1"
                     /translation="MTKRERVIAAIEGKKVDAIPSSFSLHFPKNQAVGDEAVAAHLKF
                     FKETDTDIVKVMNEHLVPYYGMIRTPKDYYELIPSFSRNTNIIEDQIEMTKKIMDGVD
                     KDAFTMGTLHGMCASGIHPLEKMGEGYNYDQVRQMQVDFLRWDEKKMLDSMERIADGM
                     CILAERYIKDAGVDSVYYAGLGAETRWYTDEEFAKWIKPFDLKIMKAIKDAGGYCFLH
                     MCKSGLNMKRYDEDYAALADVVNWGVYEAPMTLADGKKQFPGKTILGGLENRSGVLVD
                     GDEYDVRREVIKVVENFGREGFILGADCTLATEQDLKLVRAAVEQARSL"
     gene            complement(25830..28457)
                     /locus_tag="ACZPCL_00555"
     CDS             complement(25830..28457)
                     /locus_tag="ACZPCL_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR1161019.1"
                     /translation="MTEKNMWNLSPQEALKACQASEQGLTTEQAEKIRLEKGENVLLE
                     EKKKSAFQVFLDQFKDLMVVILIGAAIISMFSGNEESTIVIFAVLVMNAVLGTVQHMK
                     AEKSLESLKQLSAPDAKVIRDGRKQEIPAKDVVPGDILELEAGDMVAADGRILHNYSL
                     QVNESSLTGESTNVDKQEMALNGEAALADRTNMVYSGSLVTYGRATVLVTATGMNTEI
                     GKIAGLMNGAEERKTPLQKSLDQFSGKLAVLIMVVCALVFALCMFRKMSLLDALLFAV
                     ALAVAAIPEALTSIVTIVQAFGTQKMAKENAIIKKLKAVESLGCVSVICSDKTGTLTQ
                     NKMTVQQVYVDGKVIDGKELDLTDQTQRYLLYDAVLTNDSSIVDGKGIGDPTEYALLE
                     MFRKIQVLPDSYFGKMGMHEDALRKVMDRIEEVPFDSDRKLMSTKYRIHGVPTILTKG
                     AVDVLLDRCVSVRYGDGIRPMTEEEKEKIRLQNQHFSENGLRVLAFAYKESNEMLCAQ
                     AECGYVFLGLISMVDPPRPESVKAVAEAKQAGIKTVMITGDHKVTATAIAKQIGIFEE
                     GDLALTGMELDAMSEEELDQKIDKISVYARVSPENKIRIVEGWQRRGNIVSMTGDGVN
                     DAPALKKADIGVAMGITGTEVSKDAAAMILADDNFATIIKAVANGRNVYRNIKNAVLY
                     LLSGNTAAIFAVLYTSLMALPVPFAPVQLLFINLLTDSLPALAIGMEPADEGLLKEKP
                     RDPKEGLMTKDFMIQMAAQGVLIAAVVITAFYLGLRSSAAMASTMAFSALTLARLFHG
                     FNCRGKKSIFKLGFGTNPYSLYAFGAGALLLGLVIFIPGLHRLFQVQSMPVVSLLQVA
                     VLAFIPTLMIQMKRLICKK"
     gene            complement(28713..29594)
                     /locus_tag="ACZPCL_00560"
     CDS             complement(28713..29594)
                     /locus_tag="ACZPCL_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1161020.1"
                     /translation="METNTENWKDHLQKGGSLMIVILGNILYALAVKLFLMPAGLLTG
                     GTTGIGLALNRSFGIPVATFVLIFNVTMLLIGWKVMGRKFALTTVVSTFVYPIALGFF
                     EKVFGDLVLTQDLFLCTIFSGLGIGGSLGIVIRAGASTGGMDIPPLVLNHYFKIPVSV
                     SMYVFDFCILLAQAVGNPPEKVLYGLILVMTYTLVLDKLMLMGTTRTEVRVISERAQE
                     IREAILAEMDRGVTMLSGETGYLKQETQMIFSVISNREVPKIEKLIHTIDPESFMVVN
                     RVSEVSGRGFSMKKRYR"
     gene            complement(29770..31113)
                     /locus_tag="ACZPCL_00565"
     CDS             complement(29770..31113)
                     /locus_tag="ACZPCL_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1161021.1"
                     /translation="MGVQLDETEKRIVDLIEANRDEIIAFARDMYTHAELGYKEFKTA
                     EKFTRLMKKLNLPVKEHLAVTGVKAYLNEEKKDNVSLALIGELDALRIPEHKYANPET
                     QAAHCCGHHAQMAGVIGAAFALTDSKVKETLDGQVVFFAVPAEEYGEIEFKNQLTKEG
                     KIRYGGGKCELIRIGAFDDIDLDIVHHTEDEDICVGATSNNGFVSKVIRYKGVAAHAA
                     GAPYKGINALNAASLGLSALAYQRETFKDEDHVRVHPIITKGGNLVNVVPDEVVIETL
                     VRASNKDAIADAAFKTDRAFKAGAAAIGATCQITTAPGYLPTLKTPADEDVFAAAKIA
                     AATSEKEYTVVEKDISHHSGGSTDVGDVQHIHPVLTFNTGGKVNGLHTVDFDIVDEEL
                     AYIVTAKIFALSAYRLLRNGAVKAKEIVKNYHPVFTKEEYIQYMDSFLTYEEQKN"
     gene            complement(31234..31674)
                     /gene="rpiB"
                     /locus_tag="ACZPCL_00570"
     CDS             complement(31234..31674)
                     /gene="rpiB"
                     /locus_tag="ACZPCL_00570"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR1161022.1"
                     /translation="MKIAMGNDHTAIELKNIIKEFVESKGYEVIDLGTNSTESCDYPV
                     YGEKVGRAVASGEADLGIAICGTGVGISLAANKVKGIRACVCSEPYTAKLSRMHNNSN
                     VLAFGARVVGSELAKMITEAWLDAKFEGGRHERRVQMLMDIENR"
     gene            complement(31679..32791)
                     /gene="aroC"
                     /locus_tag="ACZPCL_00575"
     CDS             complement(31679..32791)
                     /gene="aroC"
                     /locus_tag="ACZPCL_00575"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR1161023.1"
                     /translation="MSGSVYGTIFKISTWGESHGAGVGVVVDGCPAGLPLNEEDIQKY
                     LDRRKPGQSKFTTARSEGDKAEILSGVFEGKTTGTPISIVIRNTDQRSRDYSNIMDVY
                     RPGHADYTFDEKYGFRDYRGGGRSSGRETIGRVAAGAVAAKLLESLGITVQAYTSAVG
                     PVKIDHSKMDLEEISNNRLYMPDKKAAAEAESYLEDMIQRKDSCGGVIECVVNGLPAG
                     VGEPVFDKLDAALGKAIMSIGAVKGFEIGDGFEAAASLGSQDNDCFIIGKDGRPCKVT
                     NHSGGTLGGMSDGSTLVMRAAFKPTPSISQIQKTVDRYGKEREIEIKGRHDPVIVPRA
                     VVVVEAMAALTVADLLLMSMTSRLDKIKKFFKKEEI"
     gene            complement(32796..33854)
                     /locus_tag="ACZPCL_00580"
     CDS             complement(32796..33854)
                     /locus_tag="ACZPCL_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HIR1161024.1"
                     /translation="MSGKYMAYVGSYSYTGKAKGITVFDVDVENGMFKERCEIEVDNS
                     SYVIASSDGKTLYSIADEGVVSFRILPNGSLARLNSAPIRGMRGCHLCTDEEDKYVFV
                     SGYHDGKTTVLSLNPDGSVGKIVDGVFDRGYGSVAERNFRPHVTCTRRMPDNRFVLSV
                     DNGIDQVKIYRFNDKEQRLVQVDAIRCELESAPRHFRYSKDGKFIYLMYELKKAIDVY
                     TYKTGDRAPVIEKIQTISTTSTKKPDNLTAACAMRMSVDQKYIYCTNAGENTISVYKR
                     DEETGLLTMICCLPISGAYPKDVAVFPDGRHIASVNHDSGTISFFKVDYEKGLLVMSG
                     RSIPVNEPNCCAIVKIGD"
     gene            34161..34694
                     /locus_tag="ACZPCL_00585"
     CDS             34161..34694
                     /locus_tag="ACZPCL_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1161025.1"
                     /translation="MDIGNKLKELRVLKGLTQEELADRSELSKGFISQLERNLTSPSI
                     TTLMDILQCLGTSIGEFFNEAPDEQIVFGKQDYFVKEDTEYKNEIKWIIPNAQKNTIE
                     PIYLTLQAGGSTCPDTPHEGEEFGYVLQGAVSIHLGNKTYKAKKGESFYYTADKTHFL
                     SSKNGATLIWVSSPPSF"
     gene            34758..35834
                     /locus_tag="ACZPCL_00590"
     CDS             34758..35834
                     /locus_tag="ACZPCL_00590"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1161026.1"
                     /translation="MSQPLIDLQHISKSFDGEMVLDDLNLSIKENSFVTLLGPSGCGK
                     STTLRILGGFTPPDKGNVIFDGQDITKLPPNKRQLNTVFQKYALFPHMTIAENIAFGL
                     KIKNKPKSYIDDKIKYALKLVNLSGFEKRDVISLSGGQQQRIAIARAIVNEPRVLLLD
                     EPLGALDLKLRQDMQYELIRLKNELGITFIYVTHDQEEALTMSDTIVVMNQGYIQQMG
                     SPEQIYNEPENAFVADFIGESNIVPGLMIHDELVEIFGAKFACVDKGFGNNKPVDVVI
                     RPEDIDLLKPEEGTLQGVVTHLIFKGVHYEMEVTTPDGFEWLVHSTDMFPVGQQVGLH
                     VDPFDIQIMNKPASEDEEALGVNE"
     gene            35827..36669
                     /locus_tag="ACZPCL_00595"
     CDS             35827..36669
                     /locus_tag="ACZPCL_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161027.1"
                     /translation="MNNDKKKSVAIGRALLSGPYLIWMIGFTIIPLALIFWYGITTKN
                     GNLTLANITAIMSPDHWKALWLSLGLSLISTVLCLVLAYPLAMILRGRGSSASGFIVF
                     IFILPMWMNFLLRTMAWQTLLERNGVINGILAWLHLPKQNLINTPAAIVLGMVYNFLP
                     FMVLPIYNVLAKIDDNTINAAKDLGANTLQTLFFIWLPLSLPGIISGITMVFVPSLTT
                     FVISDLLGGSKILLIGNVIEQEFTKGNNWHLGSGLSLVLMVFILISMAMIAKYDKNGE
                     GTAF"
     gene            36669..37460
                     /locus_tag="ACZPCL_00600"
     CDS             36669..37460
                     /locus_tag="ACZPCL_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161028.1"
                     /translation="MTKGKERFRKFISDFYLVIILIFLYAPIFTMMVLSFNQSKSRTH
                     WGGFTLSWYTQMFESRAIMYALYTTLLVAMISALVATVIGTVTAIAISHMKPVSKNIF
                     MSISNIPMLNADIVTGIALMLAFIAFGISLGFKTVLISHITFNIPYVILSVMPKLKQT
                     DRSTYEAALDLGASPAYAFFEVVFPDIMPGVLSGFLLAFTMSLDDFIITHFTKGAGIN
                     TLSTLIYSEVRRGIRPSMYALSTVIFVVVLVVLLIANFRKQPEKD"
     gene            37495..38592
                     /locus_tag="ACZPCL_00605"
     CDS             37495..38592
                     /locus_tag="ACZPCL_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1161029.1"
                     /translation="MRTFWAAAAAAVGSCVVSIAILGVPGAESGGGTLKVYNWGEYID
                     EDVITQFEDETGISVVYDVFETNEEMYPVVEAGGVKYDVVCPSDYMIQRMIDNNMLAE
                     LDYDNIPNAKQIGDDYWNMSKDFDPKNQYAVPYCWGTVGILYNKQMLEQLGVPKPTSW
                     TNLWDPKLSDEILMQNSIRDAFMIALKQKGYSLNTSNPEELAEARDMLIQQKPLVQAY
                     VIDQVRDKMINGEAAEGVIYSGEMLYIQEQIEELGLDYDLEYVVPEEGTTLWIDCWVV
                     PKNAENKEAAEKWINFLCRPDIAKKNFDYITYSTPNTGAYELSDEELQNNEALFPDMD
                     SPAMKNSEILRYLGDDTDNIYNEMWKEVKAQ"
     gene            38782..39180
                     /locus_tag="ACZPCL_00610"
     CDS             38782..39180
                     /locus_tag="ACZPCL_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR1161030.1"
                     /translation="MKIQESAENYLETIHMLSKHKPYVRSIDIANELSFSKPSVSVAM
                     KNLRENGYIHMDDQGHITLTQTGQDIADKMYERHIMLSNWLIYLGVDEKTAVEDACKI
                     EHVLSSQSFQAIKKHITQGNELPADMKEEE"
     gene            39254..39823
                     /locus_tag="ACZPCL_00615"
     CDS             39254..39823
                     /locus_tag="ACZPCL_00615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1161031.1"
                     /translation="MLKDLVKKCRSYRRFFQEVSIPYEDLKDMVDTARLTGCTKNCQG
                     LKFKLISTPEECAKVFPSLLWAGALKDWDGPADGERPSAYVLVLEDLSIGKNLMWDEG
                     IVSQTIMLSAVEKGYGGCMIGSCKRVEILEAVELDPQRYSLALVLALGKPKEEVRMVP
                     VGEDGSTTYYRDENQVHYVPKRALEDILV"
     gene            39893..40738
                     /locus_tag="ACZPCL_00620"
     CDS             39893..40738
                     /locus_tag="ACZPCL_00620"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1161032.1"
                     /translation="MMVKHISIEVTSKQTKKPVFGGPADLTAYILEPVDGILDKKRPA
                     VLLCPGGGYRTLSTREDQPIAMKYLAAGFHVFVLHYSLAPDVFPRALMELALAMKVIR
                     EHGDEWNVDVDRIAVSGFSAGGHLACCLGVFWDREWLYGALGVKPEMIRPDGMILCYP
                     VITSGEYCHKGSFECLMGAEASKKDEKLRRLLSLEYQVGPQVPKTFLWHTWTDQSVPV
                     ENSLLLLQALRKAGVNVEAHLYPVGPHGIALGTEETQGIDQKYLEPYCDSWMGLAIRW
                     IEKQL"
     gene            complement(40954..41592)
                     /locus_tag="ACZPCL_00625"
     CDS             complement(40954..41592)
                     /locus_tag="ACZPCL_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161033.1"
                     /translation="MSKSYHEIMEKIEVTDEMRTHILNNVSRQVSNTSHKVVTFPHLR
                     KMASIAACTALLLIGGTMMKNAFYPSSTDPNPELAGSVYGMEECKSLAELTKKAGFNV
                     KDISEADMPFKVMSTSYLWCWNEFAQIIYEGTDNSLTYRKTSGTDDISGDYNQYEQIL
                     DKVVNGVDITIKGNHDKYYLATWTTQDFSYSLSVDEGIDLETILNIIQNSME"
     gene            complement(41585..42220)
                     /locus_tag="ACZPCL_00630"
     CDS             complement(41585..42220)
                     /locus_tag="ACZPCL_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1161034.1"
                     /translation="MNSISNISILPEINSTSIWHTLVDNVLNGIAYLFAAILSTRARK
                     IETAPVGVLQNTSAIETVLDIYGNAILRLAYSYLHNMSDAEDILQDTLIKYMQSSPAF
                     LTESHKKAWLLHVAANLSKNKIDYNKIRETDELEETLVAEEREDLSFVWEAVKALPEK
                     YREVLHLYYQEGYPTKEISKILNRNESSIRSDLKRGREALKQVLKEAYDFE"
     gene            complement(42242..>42919)
                     /locus_tag="ACZPCL_00635"
     CDS             complement(42242..>42919)
                     /locus_tag="ACZPCL_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161035.1"
                     /translation="RKMSGLRMFLELRECKARSNSVSEGSKYLLTPTSYFIDTKGLIK
                     MRKLMLVLMCATTMTSMAACGAKEPVTPEVTKAVTETKTAEEQETVSETVGMPNPWTD
                     CKNLNEAEDNVGFSITVPEKIDGYTRTMIQTLDKEVIQVFYEKDDQSDSTILIRKGIG
                     SDDISGDYNTYNEKTQLDINKNQIMEQGNDGKVMLATWTDAGYTYSVSTPGLSESEME
                     NIIQQIE"
BASE COUNT        11137 a        10366 c         8687 g        12729 t
ORIGIN      
        1 gtacctcgca tgatggaaat atataccttg atcacagcct gaaggagttt attttttgac
       61 atacggccca gggctacaaa aaggcctaag ggcagtgaaa aaataagggt cactgcaaaa
      121 atctgtatac tggttcccat acctcctgcc agctgcataa ggatctgctg gatcgtcatt
      181 ttacccatat ctgtacctgc tttcaaaatg tttcttacgt gtttctttta tgtccgcata
      241 gtgcggacgt atggtagagt ctttatcata gaacaagggg gcgcagttgt caactgcact
      301 cccctaaaaa ttcattatat ctgtttcaga cctaaaactt attacttacc aatggtagta
      361 acgtcttctc cgaaccactt ttcagagatc tcttttaaag ttccgtcttt tgccatatct
      421 tccagagctg cctgaacttt atctcttaat tcggtattgc ctttcttaaa gcctacacca
      481 tactcttctg cggaaaggga gtcgtctaag atcttaaagt ctgcctttct ctgctggatc
      541 tgataacctg ctacgatcac gtccatggca atggcatcta cagaaccctg ctgcagatcc
      601 ataaatgcag tgttgtaatc agcagtagtt aaaagctgtc caaaggtagc tgtcagatcc
      661 gggtcttctt ttaatgctgc ttctgcggaa gagtctgcct ggcactctac tacttttcct
      721 gcaagaccat cttttccttc aataccggag tcatttctta ctacaaatac ctgggtgttt
      781 tccatatatg gctcggtcca ggtataatca tcttcacggc ctgtcatagt aaatccattc
      841 cagatacagt caatgtttcc agactccagt tccatatctt ttgcatccca tgcaattggc
      901 tgcgcctgat attccagacc cagacgcttt gcaacttcct gtgccagttc caggtcgaaa
      961 cctgtgtatt cattattgtc tcctaaaaat cccattggtg ggaaatcctg gtcaaaacca
     1021 acgatcagtg taccgccttc tattcctgcg gctgcagctg ttgtttcttc tgcttcagaa
     1081 gaagctgctt ctgttgttgt ctctgcctca gaagcctttg tctcagctgc tgtagtagca
     1141 gtgctgcctg atgaacaggc tgtcatggat aatgccatca tagatgcaag tgctgcacat
     1201 aatactctct tcttcataat aattttcctc ctcttatata aaatgtcctc tgagagaaca
     1261 ttaaattcac gaactttgcg cttacgaacc cgcactcacg aaccccgcac ttacgtgctc
     1321 tactgcctgc tgacgcaggc ggttcgttcg ttaatgtgct atcgttttga cacgctatca
     1381 tggtagcaca ctaaagtgtt tttgtaaaga gggaatttaa aaaaggtccg gccgacagat
     1441 gtcagtggac ctttttttcg cagatataag ttacgtgcag cgcctgacgg cgctgggtgt
     1501 gcagggcatt acagtgtata cacaaccttg ccatcgcaga tcgtatatct cactctgcct
     1561 gtcagctcag cccctaaaaa cggactgttc gttgccttgg acgcaaagct gtctaccacc
     1621 cacttttcat tttcatcaaa gataacaaaa tcagctggtg cgccttcttc catacggccg
     1681 cagtctaact tgtaaagggt tgccggatta aggctcattt tttccattaa ttctgttaaa
     1741 cttaaatgtc ccggttttac caggctggtg atgccaaggg caagggaagt ttccaggccg
     1801 gtgatgccgc ttggagcttc ggtaagaggc ttttcttttt cttctttgct gtgaggagca
     1861 tggtcagtag cgatcatatc aatggtaccg tcttttaagc cttcaatgag catctgacgg
     1921 tcccactctg tacgcagagg cgggttcatc ttagccaggg tgccatgttc taacattgca
     1981 tcttctgtca atgtaaagtg atgaggggtt acctcagccc atacatgagc ccccatcttc
     2041 tttgccagac ggaccatttc cacagaatga ccggaactga tatgctggat atcgatcttt
     2101 gcaccggtag ccagagcgat catacagtct cttgctacca gggagtcctc tgccagcgcc
     2161 ggagaaccgt aaattcccag ctggtcagac accttaccat ggttgatgcc gttctgcttg
     2221 ataaaagtct tatcttcctc atggaagctt aaaacagtat ccagtctcgc tgcctcttcc
     2281 attgcctctt ttacaaaagg tccgtccatt aacgggatac cgtcatctgt aaatcctgcg
     2341 gctcctgcct ttaacaggcc ttccatgtcc gttctttctt ttccttcaaa tcctttggag
     2401 atcgctgcgc agctgagaac atggatccct gttttctttc cttcttccag aacatagcgc
     2461 agagtttctt cgttatctac gattggcttt gtatttgcca tacacacaac ggtagtaaaa
     2521 ccgccttttg cagcagctgc tgctcctgtg tggatatcct ctttgtaggt cagtcccgga
     2581 tcacggaaat ggacatggac atctaccagt cccggtgcta ccacaagacc ttttgcatcg
     2641 atcacctggt ctgcagctgc ttctgcctct gctggtaata ttccttctga atatattttt
     2701 tcaatttttc catctttaac aacaatatca gcgtttcttt cgctctttcc tgccgggtcg
     2761 atcacccgtc cgtttttgat cagcagcatg ttcttgtcct ctcttcctta aaatgtattc
     2821 tcaaagtctt tcctgcacct gcttttgcag ccggttttct atatcaggaa actttctccc
     2881 tttttatgga tctttacgat ccggttctta tagtcttttg cacactccag tgccttaaac
     2941 cggtctgtta cttcaaaatc accccagaaa tgcataggga acaccacatc ggctcccacc
     3001 attttcataa agtcgtccat tcccagatag aaatcctttt cctgtctcgg atcaaggagc
     3061 ataaaagcca catctatatg aatgcccttc atcttctcca gctctttatg gaaattttca
     3121 ctcatccgct tattatctgc ttctggttct cctgcccata cccagttgtt cagatctcct
     3181 gcatgataga tccgctttcc ttctgtttcg atcagaaacg caacaccttc atctgtggat
     3241 ttaaaagccc ggatcttcac accggatcct cctgtaaaag gtgctgtcag ttccagttct
     3301 tctcctggac cggtaaaata ggtttttcca aggaattctt ccggcactct ccgcttccag
     3361 atatcatcag aaagtacaaa accagtcttt ttatgctttc ctgccagttc aaagatcacc
     3421 ggtgaaaaat gatctccatg ccggtggctg gcaaaaacca aaagaggctt gtcttcctta
     3481 aattccggga gttctccttc aaaatagtca aataaaaagt aattttcatc agtctctaca
     3541 aggaaactgc tgtggtggat ataagtaact ttcatgttct tccctccaga catcacaatg
     3601 ttctctctga gaagacatca caatgttctc cgattcttat tataaaaata catgcaaact
     3661 gctgtagtac agatgatcac atatccgcaa agactaagca tatccggctt ctgtcccagg
     3721 accagaaatc ccataagagc tgaaaacatg atctgcgaat agtcaaaaac agaaatttct
     3781 tttgccggag cataaaaata tgctttcgtc acaaagatct gaccgcctgc accgccaaat
     3841 ccggctgcga tcatagctgc aacctgccac catttcatag gagcaaaatg cccaataaga
     3901 aatggcagtg caaacacaca ggtaaaggct gagaaaaaca tgactatcag ctttccattc
     3961 acccctcttt ttcccagcac acgcacaaag ctataggctg caccggctcc cgcacctccc
     4021 agcattccaa taagcgctgc cgggtctgtc atatttcctg tgggtttgat gataaataat
     4081 gctcccataa atgctactac aactgcaatt ccctgaaaca gggtcagtct ttctttcaaa
     4141 agaaaataag aaaaaataat agtgaagaaa ggagacagct tatttaacat attggcatcg
     4201 gaaagcacca gatggtcaat ggcataaaaa ttgcatacaa taccaaccgt tccacagata
     4261 gaacgcagga acaaaaactt aagatccccc ttgtgtattt ccagcttttc cggcttgcgg
     4321 accaaacctg cgcctacaat aaaaaatgca agcaaattac ggaacaatat cttctgcata
     4381 gatgtcagat ctcccgccag ccggacgcac agcgacataa aagcaaaaca aaatgccgct
     4441 gtcacaatac ataaaatacc ttttgtcttg gaattcatat tttttctttt atcccccttt
     4501 tattccccat gataaaatgt tttccacttt ccactgaaaa accgtgtaac aaaaaagctg
     4561 atgcgaacga tccagtctat gatcatggcg atccacacac cgatcgctcc cagacccata
     4621 gccacacctg ttacatagct taaaccaata cggaaaataa acatagatgc aatggataca
     4681 cacatcggaa attttacatc attagccgca cgcattacat ttggaaggca gaaacctgcc
     4741 ggccataaaa agatagcaca gccattatgg atcagggcca gaacagctcc taaatgtctt
     4801 gtttcccctg aaactccata caaccgcaaa gtaacaggca tggtaaggat caccaccaga
     4861 cagcagattc cgttaatgac ataagtcagc ttcatcattt tcttcgcata atattctgcc
     4921 tgttcaaaat ccccggcacc gacacagcgt ccaattactg taatgatcgc cagagacata
     4981 gccgttcccg gaagaacgcc catactgtcc aggttatttg caatggcatt tgcagcgatc
     5041 tgtgtagttc caaacatggc gataatgctg actgtaagca cacggcccag ctggaagata
     5101 ctgttttcaa ttccatttgg gataccgata tgtaagatct gctttaccat gcgcatattc
     5161 acgcctttgg tggttccaat acggatatcc agcttaggat cccgcagtct ccacatcagg
     5221 ataaaacatg ctgtcatacg ggagatcagg gatgcacctg ccgcacctgc cacgctccag
     5281 tgaaagccaa agataaacag agcatctccg atcacattga tcacattcat gataatggat
     5341 gcctgcatgg atatcttaga gtttcccata gaccgaaaca gcgccgcaca ggagttatac
     5401 acagccagga agggatagga aatggcagag atggtcagat agatcactgc atttttcatt
     5461 acctcttctg taacgccctt ataaataagg tttaagatct gccagcgaaa caggatgctt
     5521 aaagtcatga tcaccagagc aaccacacct gttacaagga tcagctggtc tgctgactgg
     5581 caggccctat cttttcgctt ctgtcccaga tactgggaag caactaccgc accgccggtt
     5641 gccacagcag caaaaatatt gataataagc acattgataa tgtctactaa agaaacaccg
     5701 gaggtagcag cttctcctgc actggatacc atcatagtgt ctaccatacc aacggaaacc
     5761 gccagtatct gttcaatgat caacggaaag atcagctttt tcagatcatt tccggaaaaa
     5821 agagtcccca cagtttttgc tttccccact gttttctctt tttgcatcat cattttccca
     5881 gcacttcttt catttcattt tcctggttat caaacaggag ttctttgttg taacgatagt
     5941 agcgttcaca gttgtattcc tttaaaaatt ccacactggc ttcccctgta tttaaaccgg
     6001 ttacaaagat caccagttcc tggccatttg caaaggctgc ctccataaaa tcaaaggcat
     6061 gttccagccg ttccccgcag accgtctttt tatcctcata ggcatcgctt ttctccataa
     6121 actgctgccg cagccagttc cagctgtcct ccttttcctg taaggaccgg gccttcatct
     6181 gctgtacata ctgttctaag agttctttta ctttccggta aatgtgatcc tgcctgcggt
     6241 ctgaaagccc actttctttc ttgtggatgc ggatgctttc taactcagcg ccaatgcttt
     6301 ccaggatcat agcaggcgcc agggcatgaa catcagcacc atccagttcc ttcttaaact
     6361 gctttaactg ggccattaaa agagtcatag tctcattcat ccgctttaac tccttaaagc
     6421 cgtctgtcag ctttgcaaga agaagacctg ttactgccat acgttcatca aaaggtgcct
     6481 tctgcagctt gtcgcagact tcttcccgga tagtgcctga aaggatctct tccacctggt
     6541 actgatcctg atatttataa taaagttcca gataatttgc aaaatccttt gcaatcttgg
     6601 gatgctgtaa atactgccct actacatccc ggtctgtctt ctttcccagt gtctcatata
     6661 cctggatcag ctgggataga tcttcccagc ctcttggagt ggcaaaacgc ttgccgtcta
     6721 ccgtagtctc tacagtacag aaatactggg gacggatgtt cagataggac ataatagccg
     6781 ggtggatatt ctgtgcatag gcgtattctt tccacacatt taagtccggc tcaataatga
     6841 tctttttaat acggtccaat gtgaccacat caaattcccg gacggatttg ttgtattccg
     6901 gcgggtttcc cgctgctaca atgatccagc cttcaggaac cttatggctt ccaaaggtct
     6961 tacactgtaa aaactggagc atggtaggcg ccagtgtctc cgacacacag ttgatctcat
     7021 caataaaaag aattccttct tttaagccgg tctcccggat gcggtcatac acagaagcaa
     7081 tgatctcgct catggtatat tctgtaacag aatactcttt tccaccatat attttatgct
     7141 cgataaaggg aagacctact gcactttgcc gtgtatgatg ggtaatggta taagccacca
     7201 gtccgatctc acactccctg gcgatctgct ccatgatctg ggtctttcct actcctggag
     7261 ggccgatgag cagtaccggt ctctggcgga tcgccgggat cttatattct ccaaattcat
     7321 cttttaaaag ataggccgca atggtatctt ttatttcttc ttttgctcgt ttgatattca
     7381 ctggatctgt tcctttcttt atactcacga accccgtgct gacgcactct actgcctgcg
     7441 ctcattaaac gtttcttcat ccaacaccac ccgcatggcc caggcgggaa aatcttcttc
     7501 tgaaaacctc tccttaataa acacaaaggc cacatcataa gtgggcgctg ccaccggata
     7561 aatgcctttt ccgtctgtaa aataaataag cccctttaat ccatttaact gtcctgtaac
     7621 cttcagagta ttgacatact caaaagccgg tctgaaatcc gttcccccgt atcctttgat
     7681 ctgtaaatgt tccatatagg ctttcagatc atcagcacag gtaaggatca catctgactg
     7741 caccttatca tcacactgga tcacatggat ctgtaccttc cggaaatagg tttctgactg
     7801 ggaaagtaca tcataggttt cctctaaaaa ccgctgtacc agttccccgg aacaggacat
     7861 actggtgtca atgacaatga caaactgctg gatcctctgc acttccctgg tctccaaagg
     7921 ctcgatcagg ggcatatttc catacagaga aagaccgtaa gtgtaaaatg cctggtcaaa
     7981 ggagtctgga tctgtctgca tctcttccct taaaacagca aatttcttta aaaactgccg
     8041 gtaatcatac cgttcttcat tttccacctg aatggagtct aataagctct gttcattttc
     8101 tgactcttcc tcagacccca tagtctccat ctgggtctgc atccgctccc tgttgtcatt
     8161 ccactggttc tgacgggtca tagctgctga cggatccggt tcctgctccc agagatcatg
     8221 gtcatctaca tgaaactctg cttccaactg ctccatacgc cgttttggaa gttccatctc
     8281 cattaatgca tgatataccc gctctgctgt aagaactacc ttccgctctt cctcattgga
     8341 agcgccggta ttcttatttc ccgtaaggaa gcggcgcaga cgaagataca tttctctgcg
     8401 caaaggactc tggggaacat ggatgcattt ttcatacata ccgtccaaca cagactccat
     8461 ggcaatatca caggccagat cccacatttt tttctctctg ccctttcttg tatacagatg
     8521 acagaacaga caatggaaca acgtgtgcag ataataacgg tttaccactg ccctgccctt
     8581 tttgtaaaga cctaaaagaa atccgggacc ataatagatc accgcaccgt cgcaggccat
     8641 tccatttctg ttccagtctg cttcaaatcc aagactgctt aaagacacat ccaggaaatg
     8701 catatttaca tacagctcat ttctggcctg atataaaatg cgggtacata actccacctg
     8761 gtccagttct tctaattgcc tctgcctggt gggattttcc attactgtgc gcctccattc
     8821 atcagcctgt gctcttcccc aatttcctca tagagatgat aaaagctcca gcctgcattc
     8881 tgctctgtaa caatggcttt taaattgatc ttcggcacct ttgcctttct caggtttaaa
     8941 agaagggtgt cgatcctgcg gcctgtaaag tcacgcatca ccagcatcgg ttctgtgatc
     9001 tcaggcagtt tttcttctgt ttccagcgtt tctgatactt cccgcttttc aaatccgggc
     9061 acacctgcca gatatcccac actttccagt acctgatccg gggctacatt acggatcttc
     9121 acccccatgc gcaccagaac gcttttcatc atcatttcct gtttcttcat acgggcatct
     9181 cccgtactgt aatacaatac cagttccatg gctgtccttt ctgctgtccg ctttttctat
     9241 ttaaaaagct gctcactttc caacatcagt gtaaacgggc cgtcatttaa cagttctact
     9301 ttcatatctg cgccaaactg tcctttttcc actacatcca cgtaatttct gcaccgctcc
     9361 ataaaatgtt catataagtg ctctgccata tccggagcgc cggcttttac gaaacttggg
     9421 cggttgccct ttttgcagtc cgcatataag gtaaactggc tgatgaccaa cagttctcct
     9481 cctacgtctg ccagacttaa attggtcttt ccattttcat cttcaaaaat acgcagctta
     9541 cagatcttgt ctgccatttt gtcggtgatc tcttctgtat ctgaattttc tactcctaaa
     9601 aggatcataa atccttttcc aatttttccc aaaacttctc catctactgt gacacttgct
     9661 ctagtcactc tctgcagtac tgctctcact gtatcttcct cctatttcat cacctgcctg
     9721 atatatgtca gtaaattttt cgctacatcc ggctggtaca gcacttcttt tgcccttgta
     9781 ggataatcgg tgataatatt gtctacgccc atcagggcca tctgttcaat ttcagagcgg
     9841 ctgtttaccg tccatacgtg gatcgcttta ccatattcat gagccgcctg gatctgcttc
     9901 tttgtcacaa ggctggaccg gatgctgata aaatccacaa attcattttt ataataagaa
     9961 ccataggctg ctgccaggat ataacctgtt tttaactccg gatctgcttc cttcacccgt
    10021 ttcagataat ccagggaaac agaggtgatc acacactgat cttccattcc catttctttt
    10081 atcatttctg ttactacctc aggaagcggg cttttggtgc ctatgctttt taattcgata
    10141 tttaagcgga tacgaccttt gcaggcttct aagacttctt ctaaagaagg gatcttctct
    10201 ccttcaaacc gggaactgaa atggcttccc acatctaact gctctaactc atcaaaggta
    10261 aggtttttaa gagacacatc tacacctgcc acacgggaca ggttcagatc atgacagacc
    10321 accggcacgc cgtctttaga cagctgcaca tcgatctcgc tgtaatctgc catttcaccg
    10381 attgcttctt caatggctgc catggtattt tccggtgcgt gtttggagct tcccctgtgg
    10441 gctgtgatct ccacctggga caagatcgcc tgatccggcg aatttccatt atacaccata
    10501 tcaaaaacca tgaaagcggc aattcctgaa aatacagctg ttaacgtcaa aaaccagcgc
    10561 tttctggaat agccttccct gttttttgga aaatcatatt cctttgcagg aactggcact
    10621 gtgaaaccaa actgatgata caaaactgtc atggctccaa aatccgtcac aactgccatc
    10681 acagagccta aaaacaggat catccattcg atccggtaac tgaccgttgc cataactgcg
    10741 gtagctgtgt aagcctttgc aaaaagagtt accagtatgg cggcgatcac cataactgcc
    10801 ccataaagaa ggaccagccc caggatcaga aacaggttta atactaccag cagtaagacc
    10861 gccctgggcc attttccctt taagatctcc cggctttctc tgactccgtc cctgaaattt
    10921 ttctgttcca gcatacaggc gaaaaacacc atcatagccg ggacagaaaa tacagtcaga
    10981 agaacgcatc ccactaccag agccattctg gtccctgctg cgtgtaagat ctcatataaa
    11041 acaaagttta ccggcttcat acgggtcagg atccgtacca gaaaataaca gttcatcagg
    11101 atataatttc ccagagccgc taacagaagt ttcccgtttt ttctgcaaag ttcattcttt
    11161 gccttggaaa gtcctcccag gaaaatggat actgcactga tctttcttaa ataaatagaa
    11221 gaatgatacg ctgttatcat tgcccctgtt tcaataagaa gaaagatcat tccaagaaga
    11281 aggataaaga tcacaaaagg aatagtaaat ggatgcagca gaaaggccct cagattgcct
    11341 atggtaaggt aactgtaccc tgccatatgc agtgagaacc ttaaaatacc atttaccgtc
    11401 ttaacataga atatacctgc aaccagccgg tataagaaag taaataatac cagctggcgc
    11461 aggttctgtt tgatcacatt atctgttaat tttaaaaata atttcatatt tattcccgat
    11521 ttgggtctgt ttccggatgt tcttttgcat aggctgtaat tgcctcattt aaagaaaaca
    11581 tctttttatc cagcatatct accacatctg cacattcttt taattcccgc agcagtcctt
    11641 ccggcgcatt gccctcaaac ttataggcga tcttatgctc caagctggcc cagaaatcca
    11701 ttgctacgga acggatctgg atctccacct tggtctgtac aggtccgtca gcaagatata
    11761 caggaactgt aactaccata tggtaacttt tgtatccgtt gggctttgga ttttgtatat
    11821 agtcttttac atggagtacg gtaatatctc cctgggctgc gatcatatct gcgatctggt
    11881 aaatatcaga tgtaaaggag cagatgatac ggatccctgc aatatcgctt aaatgatcca
    11941 tcatattttc aatggtcact tcataaccgt cttttttcag cttctttaca atactgtctg
    12001 ctgttttcag cctggattta atatgttcga tcgggttgtg gtcatagcgg ttgacaaatt
    12061 cgttgttcag tatctcgatc tttgtattca gcttctttaa agccgagtca tacagaaaca
    12121 tcacggaccg ccactgatct acctgatctt ttgttttggg gattactgac atcccggccc
    12181 ctttctacag cttcagtcct tttaacagat ctcccagtcc tgttgttgca gctgctgttt
    12241 ctttataatc aaatgtttcg tgttcttctt cctcagtttc ctgttcgatt agtgccttca
    12301 tgctcaggct gatctttccg tctgcaatag aaaggatctt aacttttaca gtctgtcctt
    12361 cttttaagac tgcacctgga tgtttaatgc gctgggtgct gatctgggaa atgtgtaaca
    12421 gtccgtcaat accgtctgcc agctttacaa atgcgccgta gtccttaata ttatctacag
    12481 ttccttctac tacatcaccg gccttaaact gagccagacg ctctttacgc tgtgctgcct
    12541 tcttttcttt ttctacttca cggccggaaa gaactaatct cttcttttcc ggatcaaccg
    12601 tgatgatcac tgcatccaca tgttttccct gccagtcttc cagtttttca acatattctg
    12661 tagacagctg ggaagccggg ataaatgctc ttacttcatt tacaaaagca actacgccgc
    12721 cttttactgc ttcagcgatc tttacattta atacagtgcg gtctgccatc atctgtgcaa
    12781 actcagccca cttttcgcct tctgcatcat ccgtttccgg aagttcaaaa tgtctctcgt
    12841 caaatgcttt ataagatgct tccagttctg cagcatagtc ttccatggtc tctactggtg
    12901 cttctgcttt tacttctgct tcctgctgtt tgttcatttc ttcgttcatt gttttttcct
    12961 ccgctgttct attttctagc gtaaactcat gacccccgcg ctgacgcgct ccactgtctg
    13021 ctgacgcaga cggttcattc gttaatggtt cgtgaaaatc atcggacgcg tcactggcgc
    13081 ctcctcgata tgctaggcaa caaatgtctg cttcgcagcc gcttgttttt cccttcactc
    13141 attacattat aacctaccat agttacacat ttccatagtt ttcttgggaa aattgcgcca
    13201 gttttttctt tttttcgctc ttttctccct taatttgcta tgctatactt gctctagtat
    13261 ataatttcat aatttgatac cagggtcaaa agatctaaaa tccgacgcaa gcttgcttgc
    13321 aggaggattt ttgaacttgg gacccgccaa aaacatgagt aagcagccat tttttgaaga
    13381 aaaatgagcg aattacgaat gtttttagaa ttgcgagagt gcaaagcacg tagcaattcg
    13441 gtatcagagg ggtcaaaata ccttttgacc ccaacattat aatttaagag gtattttaca
    13501 tccatgaaag atattttaga ccttcacaca cataccattg ccagcggtca cgcttacaat
    13561 actttatatg aaatggcaca ggctgctgct gataagggac tggctgttta cggctgttca
    13621 gaacatgctc cgtctatgcc cggtacctgc catgaatttt attttactaa ctttaaagtc
    13681 cttccaaggg tgatccgtgg tgtcccggta cttatgggcg cggaactgaa cattctggac
    13741 tttgagggac atgtggatct ggatgaatac tatttaaaaa agatcgacta tgcagttgcc
    13801 agcctgcata ccccatgcat cgacaacaat ggttctgttg ccgactacac aagagcctat
    13861 ctgaatgtac tggaaaatcc ttatgtaaac attatcggac acccggatga ctcccggctg
    13921 cctgctgact ggaccgaagt ggctaaagca gctgccagaa accacaaatt actggaagta
    13981 aataatactt ccttaaatcc tttaggcgcc cgaaaaggtg caaaggacaa ttataaagaa
    14041 cttttagctg catgtatccg ctacggcacc tctatcatca ttgacagtga tgcccactgc
    14101 gaggcagacg taggaaatca cagtctggca catgcatttt tagaagaaat gcactttccg
    14161 gaagaactga tcgttaatac ttctttagaa aaggcggctg cttttatccc taaattaaaa
    14221 caactgcttt ctgaaaacga agctttccgc gcttccgcag aaaggaacac tgccctatga
    14281 tcaactttct gaacctggaa tattttctgg tggctgcgga ggaattaaac ttcacccgcg
    14341 ctgccaaacg gctttatatt tcacagcagt cactcagcaa ccacatttcc aacctggaaa
    14401 aagaatttga cgttgttttg ttcaaccgca cctctccatt gacactgacc tatgcaggac
    14461 aggcgttaaa aacaagggca agggaactgc tggacctgag agatgaaaca tataaggaaa
    14521 tttcagatat caaggacttt tccacaggac agctttccat tggcgtttcc cacaccagag
    14581 gccgctttat ccttccggag atccttccta cttatcagag ggaattcccg gggatcgaac
    14641 tgcacctggc agaaggaaat tccagtgagc tggcaaatga cctgcttcac ggaaacattg
    14701 acctgatgat cgacctgctt ccctttacag cagaaaatgt ggctactgtg cccatatgcc
    14761 aggaagagat ccttttagtt gtcccggacg aggtgatccg aaaagcctat cctgaccgtt
    14821 ttgaggagat caagaaaagt ctttaccagt ctgcagatgt gcgccttttg gaaaactgcc
    14881 cttttgttct gttaaaaaag ggaaaccggg tacgtaccat tgcagatgag atctttgaag
    14941 atacccagat ctctccccat attgttctgg aaacagaaaa tattgagacg gttctggccc
    15001 tcagtgccca gggaatgggg attaccttct acccccgtat gttcatgtcc accagacaga
    15061 atttaaacgc atataaagac tcccttatgg gacaggcagg actgaacctg ttttccttga
    15121 attattccag agcccacggc acccttgcta tcggctatca caaaggccac tatatgtccc
    15181 gtgccaccca cgaatttatc cgcattgcac tggaacatat cccttctgcc gcaggacttc
    15241 cgtcaaattc agatgcaatc tgatgacctg ggagcctgaa agaaaacgaa gatcaccctt
    15301 gactttcgtt ttcttttttg ctatattata acagtaatca ttatcattat taaatttcat
    15361 caacaaggga gacgcctatg aaaacattaa agtatagccg tcagagagaa tccatcaaag
    15421 ccaacctgat gagcagacac gatcatccga cggcagacgc cctatacgcc agtatccggg
    15481 aagaatttcc aaatatcagt cttggaactg tatatcgaaa tctgaacctg ctggtggaga
    15541 cgggtgagat cctgaaactg acctgtggaa acggcccgga ccattacgac ggaaacgtga
    15601 cgccacatta ccactttgta tgcagagact gcggacaggt ctatgatatg gaactggacg
    15661 aaatggctga tctgaacact tccgttcagg ctgctgcccc tggaaagatc gacagtcatt
    15721 ccattttatt ctatggaaga tgctactcat gcatggaaaa agaagagaca gagaatactc
    15781 ttaagaaaag cagctaaaaa acagtaaaaa aaagagaaat ttataaaagt aaatgaaaat
    15841 ttctcttgac aaacaagaca aaatgcatta tattaatatc agtaatcatt actattattg
    15901 attacaagag atcaaacgaa acaggaacta aagtttccga tcacattaaa tcaaaaaaac
    15961 gaaaaggaga atatcattat gaagaaatgg gtttgtacag tatgcggtta tgtttatgaa
    16021 ggagaaaatc cacctgaaaa atgtccacag tgtggcgtac cggcttccaa gttcaaagag
    16081 caggctgctg acgagaccag ctgggcatgc gagcacgaag taggtgttgc acagggttcc
    16141 ccagaagata ttatggctga tttaagagca aactttgaag gcgaatgttc agaagttggt
    16201 atgtatctgg ctatggctcg tgttgctcac agagaaggct atcctgagat cggtctgtac
    16261 tgggagaagg cagcttacga agaagcagag catgcagcta agtttgctga actgttaggc
    16321 gaagttgtta ccccttctac caagaagaac ctggaaatga gagttgctgc tgagaacggt
    16381 gctactgctg gtaagtttga cctggctaag agagcaaaag cagctaacct ggatgcgatc
    16441 catgacactg ttcatgagat ggcaaaagac gaagctcgtc acggaagagc attcgcaggt
    16501 ctgttaaaga gatactttgg ctaatctgat tttgcaatgc atttgaattc ttaaagaaaa
    16561 agaaccggct cacaacgaac cggttctttt tatatgtaca taaatgatat tcaattaaat
    16621 gttcatagaa tgcagtgcca ggatctcttc cttcactgcc agtatatctt tttccagata
    16681 cacacaggcc tcttcaatgt tctgtttttc cagagcatca acaacggcca tgtgataacg
    16741 tccattagac tcattttttc tctgccacgc agacaggaaa tactgtgaga tataacggga
    16801 acactggtgg atcatctgct gtaatgcctg atggccgtaa acattgttgt ttaatgaata
    16861 cagataggtg tgaaactcat aatttctctg ccagttgttt gatacgatcc ggtcctcatc
    16921 tgcattatgc ttttctgcga tcatccgaag catatttata ttttcccggg ttaaaacagg
    16981 aaagcttttg cgaaggcctc ctacttccac atcaactctg aattccagaa tatccatgat
    17041 ctctttcagc gtaactgccc gcacctgata gcccatacgc ggaatgttga tcaaaactcc
    17101 atctttacag agttctacca gtgcttcacg aacaggagac ttgctcacac caaatttttc
    17161 aatcatttga ttttcagtaa gaatatcatt cggtctatat tcgccgtttg tcacagcttc
    17221 gtaaatcccc cggtatactc tctcttttaa ggtttctttt tgcatacatt cccctaagct
    17281 acatatttag tagtgctttc gcacccaata tgctattttc tacttaaaaa ccgattttgt
    17341 caagttttta atcaaccact tcatccagtt tctggatcgc ttctacgtag atcacaaaag
    17401 ccttctttaa atgttcgatc tcaatatatt catctgcctg gtgagcaccg cctctttctg
    17461 taccaaagcg ttttgctttt ccaggaatgc caggtccaaa tccaactgca tgttttaact
    17521 ttctggaata agtaccgccg cccattacat atggctctaa ttccattccc agattgcggt
    17581 tgcatacttc cattaatgcc tgtacttcct tggatttagg atctgtaaag catggcgcgg
    17641 agtcatgggc ttctgtcagt tcaaagccat gtgcctttaa agttttcgtg atgttttcca
    17701 tcagtacttc ataaacagct gttacattat aacggatatt tacatcctgc cagaaaacac
    17761 cattttccag cttgcacata ccgcctacat gagtcagctt gccggaacct tcatcttcaa
    17821 aaggaatgtt cagtcctgct ccatagtaat caccaaacat ctcacagatg gcatccatca
    17881 ggtttgctgc atcttcatct aacacgcctg attttttcag cagtcccgcc atttttactt
    17941 ctgcgctttc agaaccttcc gggaaagctg catgagcagg aataccatgt acatatacct
    18001 tcacactgcc gtcagcctgc ttttcacagg tgccgccaac tgcttctaca acctctttta
    18061 cttttgaagt ctcttcttct gttaaagtaa gaactgcaac tgcttctgca ggaacctggt
    18121 tggacatgat accagactca aagcttacca gcttgctgct ctttaccgga cgggtagcat
    18181 caaattccag aattcctttc tcaccgttgc atacagggaa cataacgtcc ggaacaatgg
    18241 aaaatgccgg ctctttataa ttcttcgcat aatactgcac atcggccatt cccttttcct
    18301 cgtcacatcc aaagaaaaag cggaagctgt gttttggctt atagccctgt tctttcagat
    18361 attttaaagc ataaagggca gtcataaagg aacccttatt atcgcttacg cctcttccga
    18421 tgagaaggcc gtcttttaca actggcttaa atggttcgca gcatgtccag ccatttccgg
    18481 caggtactac atctgcatgg ccaaagaaac cgatctctgt atcactttcc cctttccaga
    18541 gaagagttcc gcagtaattt tcatggttaa atgtttcaaa tcccatggat cttcctaaat
    18601 ccagagctgc atgaagggca tctaagcatg cctgtccaaa aggcatatcc ggatcttctg
    18661 tttttacgct tacacttggg atctctgata atttgcatat atcctgaacc atatcatcaa
    18721 tgtgtttttc aatccattct ttttccattg cttttccctt tctttttata taaatttggt
    18781 atcaaaaaac aattgccgct tatagcggcg cttgttttta ttttacatca tcatataaat
    18841 ggcacgctac acagtgattt tctccgatct ggcgcatctg cggcacttcg gtcttgcagc
    18901 attccattgc aaatttacat cttgtgtgga acagacagcc ctttggcggg ttgtacgggc
    18961 ttgggatatc tccctctaac agcattggct catgctttac atctacatcc ggtaccggaa
    19021 tggcagaaag cagtgccttt gtgtatggat gcatcgggtt gtcatataat tcctgtctgt
    19081 ctgcgatctc aaccatacgt cctaaataca taactccaat gcggtcacat aagaaacgta
    19141 ccacacttaa gtcatgggaa atgaacatat aagttaagtt aaattctttc tgaaggtcct
    19201 tcatcaggtt taatacctgt gcacggacag atacgtccag agcagatacc ggctcatcac
    19261 agaaaatgaa cttcggctct aagatcagcg ctcttgcaat aacaattctc tgtctctgtc
    19321 caccggagaa ctcatgagga tatttactca tgtattcttc acgaagacct actttcgcca
    19381 tgatattctt tacttttgcc agtttttctg cttctgactc atgagaatat acatcaagag
    19441 gagcctggat cagctctctg actgtcattc ttgggtttaa ggatgcataa ggatcctgga
    19501 aaatgatctg gatctcctgg cgcagcttac gcatctcttc accggaaaga gttgccagat
    19561 ccgttccctt ataaaggacc tgtccactgg ttggatttaa aaggcggatg accgttcttc
    19621 ccagagtgga ttttccacat ccggactcac ctacgatacc gaatgtttca ccttcattaa
    19681 tggtaaaaga aacatcattg acagccttta caaccggtct gtctttctga aacagttttt
    19741 tatttccata aaaaaatttc tctacatttt ttacttccag gatcacatta tctttcatcg
    19801 cttacaccgc ctttatcttc atacaggaag cacctgacgc ttctctcacc aaactgatac
    19861 attggtggct gctcattccg acatttttcc attgcacagg tacagcggtc gcagaaacga
    19921 catccctttg gcatggaagt aagggaaggt acggttccct caatggtgta aagccggtca
    19981 atatctttgt cttttcgtgg aatagatgcc agaagtccct ttgtataagg atgttttgga
    20041 tctttaaaga tctctcttgc agttgcatat tcaatgacca tacctgcata catgaccatg
    20101 atcttgtctg ccatctctgc tacaacaccc atatcgtggg tgatcagcat aatggaaact
    20161 ccggtctttt ccttcatttc cttcatcagt tccatgatct gcgcctgaat ggtaacatcc
    20221 agtgcagtgg ttggctcatc ggcaatgata agagatggat tttgtgccag cgccatggcg
    20281 atcatcacac gctgacgcat accaccggaa agctgatgtg gatattctcc atatctcttt
    20341 tcaggagaag ggatacctac ttttgtcagc atttcaatac tgcggttttt tgcttcattt
    20401 ttatcacagc tgtgatgtac gataaatgct tcatctagct gttttccaat ggtctttacc
    20461 gggtttaagg aagtcatgga gtcctggaag atcataccga taccggttcc tctcacctgg
    20521 gccatttcct tatctgaata gtttgcaatg ttctttccgt tcaggatgat ctcatctgcc
    20581 tctatttttg tggttgcctt aggtaaaagg cgcatgatcg acatggaagt tacactctta
    20641 ccgcagccag attcgcctac gatgcccagt gtctctcccg gatctacatg aaagcatacc
    20701 tgttctacgg caggaaccca cttcttgcca attttaaagc tggttttcaa attcttcact
    20761 tctaataaag gttccatgtt catcctccat ttcgctacac gttctttcaa tctttttgtg
    20821 aacgcattac ccctcttctg tggtgctgca gaccggagca gcttttgcct tctgcttcgg
    20881 tctgcaccgc cacacaaggt ggcgggcaat gcctatatta taaacattta ttacttttcg
    20941 atggtccagt tccatacgtc tctgtttaac tgagagtaat cctggttgtc ttttacatta
    21001 ccaattctgt tgttgtaagc aaacaggttg ttctggaaat acaggaagca gaatggtacc
    21061 tgctctacca gcagttcctg ataattgtca tagttaactt ttctgtcttc aaagctgaat
    21121 gcatgctcgc cttctgcacc ggtgttgtaa atggtccagt cagacagatg agagaagttg
    21181 ttgatgtgat ctggcttgaa gttgattaca cactcagatg ggtcaggtga accgccggaa
    21241 ccgatcagac caaggtcata gtttccttct cttactttgg ttaagtgggt tgcgaagtca
    21301 gcagactcga tctttgtctt gatgccgatc tcctgcaggt tctgctggat gataactgca
    21361 gcctgctctc tgatgctgtt acctgtaggt acagagaata ctaattcgtg ggaaccatcc
    21421 cagccagctt ctgctaacag ctcttttgcc ttttctgggt tgtatcctac gtttactaca
    21481 tcatcatagt acttgtggct tgttgcaaat ggtccaaatg ccggctcgcc ttcgcccttt
    21541 aacagaccgg aaacgatcgc gtctctgttg atggccatgt caattgcctg tctaaccttt
    21601 ggtggcagat aatctttttc ggtattgatc gccatgtact ggtatcctac agactctgta
    21661 gatacgcagg tcaggttctg ctgctgctgt gccatatccc agtcagataa ctgaagggaa
    21721 gcaacgtttc cagccaggat gtcgatctca tcgttcatca gaccggacag aaggctaggt
    21781 gtagcaacaa cttttactac gaaacgatcc cactgaggag tctttaagta gtagtctttg
    21841 tttgccttga attccatacg ctcgccgctg atctggctct catagataca tggaccggaa
    21901 ccgattggtt tctcccagta gctgttgttt ctgatatcag catatggtgt atcgcctaac
    21961 agatgctttg gaaggatata gatatctctg aacttgataa aaaagaggaa atccatattg
    22021 gtaggctctg cacaggtcat ttctactgta tgctcgtcta ctgcctttac ctgaaggctg
    22081 tcatcgccct ctttaaatcc tgcgaatggg cttacgttgt tctgtcttac aacagctgcg
    22141 cttggggagt tgtaaacctg catggtgaat actacgtcat ctgcagttac aggctcgcca
    22201 tcctgccatt ttgcattttc attcaaatgt acagtcagca ccttgttgtc atcgctcatg
    22261 ctccacttat cagccagtct tggctcataa gtaccatcct gtttcaagta taccagcttg
    22321 tcatagatca gatccaaaac tgcatctgag taagaacttg tggtatcaaa tgggataatg
    22381 gagtcccatg cagaaaccat tgccatggta atggtgccag gttctgctga gctttctgag
    22441 ctggcagctt ctgtagctgc ttcgcttgtt tcagttccag ctgctgccgg agccgctgtg
    22501 gtagctgtct gtgatgcaga gctgccgcaa cctgccagtg cagatgctac taataacact
    22561 gacacagcct tctttaaaag gttacttttt ttcatgtttt tcctcctttt cgtaagatgt
    22621 cctctcagag agggcataaa actatatagc gttaaatctt catcttggta tcaagagcat
    22681 cacggatgcc gtcaccaatg aagttaatgc tcaatacagt aaggaccagt acaacaccgg
    22741 cgggtaccca catatacagc tttgtggaca ggacactgat ggactgtgca taatacagaa
    22801 tgtttcccca ggaagcttcc ggaggctgta cgccaagtcc taagaaactt aaggatgact
    22861 ctgtaatgat cgcctgagcg gttctgaatg taaaggaaat gataaccggt gcaaatgagt
    22921 ttggcagaac gtaccgaagt aagatcgtgc cgttctttgc accaattgct tttgctgatt
    22981 ctacgtaatc cttttccctg acagacaata cgcttgcata gatcagtctg gcaaactctg
    23041 tccagccaag aaatccgatt actaatgtaa cagttgttac ggactgtcct aaaacactgg
    23101 ctaaaaccag gatcaggatc atgttaggga atgacatgaa gatgtctgca catctcatga
    23161 taacggtttc cactgcgcct ctgaaatatc cggcaaacaa tcccaacgga acaccgatgg
    23221 ccaaactgat cagtgcggaa cagataccaa ccatcagtga tacacggcca ccaaagataa
    23281 gtctggcaaa taagtctctt cctacgtcat cacagcctaa cagatgttca gctgaaggag
    23341 caccgccaaa tgccatgtag tccatggaat aaggatctaa cttcatgatc gatggaagta
    23401 agacaactgc cagtacttcc aggatcagta cgatagtgcc catcatagcc agtctgtggg
    23461 ccaggaattt tttgactacg tccccgaaat aggagcttct gttattttct ttcgccatta
    23521 cgtctactcc tctcctatga ataacggatc ttcgggtcta agagaccata aacaagatct
    23581 acgatcatgt taccgatcag tacagctgct gcaatgaaaa cagtgatacc catgatcacc
    23641 ggataatcac gggagttgat ggaaagtacc atcaggcttc caagtcccgg ccatgcaaag
    23701 atctgttctg taacaacagc gccgcctact aagaatggga tcatcatacc aatacgggtg
    23761 atgatcggga taaatgcgtt ctgaagagca tgtttaaaaa gcactctgcc ttctaacgcg
    23821 ccttttgctc ttgcagtacg gatgtaatct tcctgaagta catcaagcat agcgcctcgg
    23881 gtctgacgga tcagacttcc tacctgctgg atggaaagga ccagcattgg aaggaatagg
    23941 tgatgtaaca gcatcggaag ggttctgaca cctgatgtat cgtacatacc tccacttggg
    24001 aagatcttca atactacaga aaggagatat acaaatacca gtcctgcaaa gaagtttggg
    24061 atggctgctc cgataaagga aaacgcagag gaaatgtagt cccaggctgt ataaggcttg
    24121 tatgcggaca tgattcccag aggaattgcg atcaggatgg agaacaccag agaggacatg
    24181 gtaaggagca gtgtcggtcc gatacgctcg gagatatctg cccaaacggc gtgtcctgtt
    24241 cggtaagatg cacccatatt tccatgcagg agctgtacca gccagttaaa atactgtatc
    24301 atgaccggct ggtccagtcc cagctttgct ttcatcgctg ccatctgttc tgctgtggca
    24361 ttaggatcat tgaagatcat ttccagaggt gtaccaggag ccattgaaga aagcacatat
    24421 actaaaaccg tgatccccaa aaaagtaggt attgcaatta atattctttt tcctatatat
    24481 ctgagcattt gcatccctct tttcttaaga tctgtaaatt ataagcttct tgcctgctct
    24541 actgctgctc ttaccagctt cagatcctgc tcagttgcca gtgtgcagtc tgcgccaagg
    24601 ataaatcctt ctctgccgaa gttctcaaca accttgataa cttctcttct tacgtcgtac
    24661 tcgtcaccat ctaccagaac accgcttctg ttctctaatc cgccaaggat ggtctttccc
    24721 gggaactgct tcttgccgtc tgccagagtc attggtgctt cgtatacgcc ccagtttaca
    24781 acatctgcca gtgcagcgta atcttcatcg tatctcttca tgttcaggcc gctcttgcac
    24841 atatgtaaga agcagtagcc gcctgcatct ttgattgcct tcatgatctt taagtcaaat
    24901 ggtttgatcc attttgcgaa ttcttcatca gtgtaccatc tggtctctgc gcctaaacct
    24961 gcatagtata cagagtcaac gcctgcgtcc ttgatgtatc tttcagccag gatgcacata
    25021 ccatctgcaa ttctttccat agagtctaac atctttttct catcccaacg gaggaagtct
    25081 acctgcatct gacgaacctg atcgtagttg tagccttcac ccatcttctc taatggatgg
    25141 ataccggaag cacacatacc atgtaaagta cccatggtga aagcgtcctt atcaactccg
    25201 tccatgatct tcttggtcat ttcgatctga tcttcgatga tgttggtgtt tctgctgaag
    25261 gatgggatca gttcatagta atcctttggt gtgcggatca ttccatagta aggaaccaga
    25321 tgctcattca taacctttac gatatcagta tcggtctctt tgaagaattt cagatgagct
    25381 gctactgcct catcacctac tgcctggttc tttgggaagt gcagagagaa tgaagacggg
    25441 atggcatcta ctttctttcc ttcgattgct gcaattactc tttcgcgctt tgtcatttta
    25501 ttaatcctcc tataatattc tttttaatat tatctttaat attattacaa ccattgtagt
    25561 ccttatccgg cccaatgtca acaagtttct ttatactttg cacaacttat tttttgtaag
    25621 cactttcttt ttgctcattt cgcacaaaac ttataaaacc tgccgctgga agctttttcc
    25681 agatgcatga caacataaaa gagcagctaa cttaatagct gccctttata ttacctactt
    25741 ttccagtagg tttcaagttg tttttgtgtt ttttacaaat ttttacattt ccgatagatc
    25801 tgctctgtat ttacaattgt ttttgcacat cattttttgc agatcagccg tttcatctgg
    25861 atcataagag tcgggatgaa cgctaaaact gccacctgga gcagagatac caccggcata
    25921 ctctgtacct ggaacagacg gtgaagtcca ggaatgaaaa tcaccagtcc taaaagcaga
    25981 gcacctgctc cgaaagcgta aagactgtaa ggattggtgc caaagcccag tttaaaaatg
    26041 gatttctttc ccctgcagtt aaatccgtgg aacagtcttg ccagtgttaa agcggaaaat
    26101 gccatggtag atgccattgc tgcgctactt cttaatccca gataaaaagc agttatgact
    26161 actgctgcga tcaacacacc ctgggctgcc atctggatca tgaaatcttt tgtcatcagc
    26221 ccttctttcg gatctctcgg cttttctttt aaaaggcctt catctgccgg ttccatgcca
    26281 atggcaaggg ccggaaggga gtcagtcaga aggttgataa acagcaactg caccggagcg
    26341 aaaggcaccg gtaaagccat aagggaagta tataatacag caaagatagc agctgtattg
    26401 ccggaaagaa gatacagcac tgcattttta atatttctgt atacatttct tccatttgcc
    26461 acagctttaa tgatcgtagc aaagttatca tctgccagga tcatggcagc agcgtccttg
    26521 gaaacctctg ttcctgtaat tcccatggca acaccaatat ctgctttttt cagtgccggg
    26581 gcatcattca caccgtctcc tgtcatggaa acgatatttc cccgtctctg ccagccttct
    26641 acaatgcgga tcttattttc cggtgaaact ctggcgtaaa cggatatttt atcaatcttc
    26701 tgatccagtt cttcttcact catggcatct aattccatac cagtaagtgc cagatcccct
    26761 tcctcaaaaa tgccaatctg ctttgcaatg gctgttgccg ttaccttatg gtcgcctgtg
    26821 atcataactg ttttaatgcc tgcctgcttt gcctctgcca ctgcctttac agactccggt
    26881 cttggaggat ctaccatgga gatcagtccc aggaatacat atccacattc tgcctgggcg
    26941 cagagcattt cattggactc cttataggca aaggccagaa cacggagacc attttctgaa
    27001 aagtgctggt tctgcaggcg gatcttttct ttttcttcct ctgtcatagg acggatccca
    27061 tcaccatagc gcacagatac gcagcggtct aacagaacat ctaccgcacc ttttgtaagg
    27121 atcgtaggca ccccgtggat ccggtatttt gtactcatca gtttccggtc agagtcaaaa
    27181 gggacctctt ctatacggtc cattaccttt cttaacgcat cctcatgcat tcccattttt
    27241 ccaaaatagc tgtccggaag gacctggatc ttacgaaaca tctccaaaag agcgtattct
    27301 gtcggatcac caattccttt gccgtctaca atgctggaat cattggtcag tactgcatca
    27361 taaagaagat aacgctgtgt ctggtctgta agatctaact ctttcccgtc gatcaccttt
    27421 ccatctacat aaacctgctg taccgtcatc ttattctggg tcagtgttcc tgttttgtca
    27481 gaacagatca cagatacaca gcctaagctt tccactgcct ttaatttctt gataatggca
    27541 ttttcttttg ccattttctg ggttccaaat gcctggacaa tggtaacgat ggaagtcagt
    27601 gcttccggga tcgctgctac tgccagggct actgcaaata acagtgcatc cagaaggctc
    27661 attttccgga acatgcaaag ggcaaacacc agggcacaga ctaccatgat caggactgcc
    27721 agttttccac tgaactgatc caggcttttc tgtaacggtg ttttccgttc ttctgccccg
    27781 ttcatcagcc ctgctatttt accgatctcc gtattcatac ctgttgctgt aacaagaaca
    27841 gtggctcttc cgtaagttac taggctgccg gaatagacca tattggtgcg gtctgccagc
    27901 gctgcctctc catttagtgc catctcctgc ttatctacat tagtagactc tcccgtaagg
    27961 gagctttcat tgacctgcag ggaataatta tgaaggatcc ttccgtctgc agccaccatg
    28021 tccccagcct ctaattccag gatatccccc ggaactacat cttttgccgg tatttcctgc
    28081 tttctgccat ctcttataac cttggcatcc ggcgcagaca gctgttttaa actctctaag
    28141 gacttttctg ccttcatatg ctgcacagtt ccaaggaccg cattcatcac aaggactgca
    28201 aaaataacaa tggtgctttc ttcatttcca gaaaacatgg atatgatagc tgcaccgatc
    28261 aggatcacta ccatcagatc cttaaactgg tctaaaaaga cctgaaaagc acttttcttt
    28321 ttttcctcta agagtacatt ttcccctttt tccagacgga tcttttctgc ctgttctgta
    28381 gtcagtccct gttctgatgc ctgacaggct tttagcgcct cctgcggtga caggttccac
    28441 atatttttct ctgtcataat gttctctcct ttgttttagt atgatcggaa atgttccaga
    28501 aaaacaaaag agcgggactt ttattgcacc aaaaatatgc aataaaagtc tcgctccata
    28561 tacatgtgta ctgcccggct gcaggcctaa tgtatcttca ttatcctgcc gcgtgatgac
    28621 gaccagcaca acacagaaaa atctgtgtga actactccct gatatctaaa cttaaaataa
    28681 cacagcagac tgcaaaatgt caacggtgaa atttatctgt accgcttttt catggaaaat
    28741 ccacggccgc ttacttcgct gacacggttt actaccataa agctttccgg atcaatggtg
    28801 tggatcagct tttcgatctt tggaacctcc ctgttggaga ttacactgaa gatcatctgg
    28861 gtctcctgtt ttaaatagcc ggtctcgccg gaaagcatgg taacgccgcg gtccatttcc
    28921 gctaaaatag cttcccttat ctcctgggca cgttcactga tgactcttac ttctgtacgg
    28981 gtggttccca taagcatcag tttatctaac accagtgtat atgtcattac caggatcagg
    29041 ccatacagta ctttctccgg tggatttccc actgcctggg ccagtaaaat gcagaaatca
    29101 aacacataca tggaaacgga aacaggaatt ttaaagtaat ggttcaacac cagcggcggg
    29161 atatccattc ctcctgtgga agcccctgca cggatcacaa tgcctaaaga accgccaatt
    29221 cccaatccgg aaaagatggt acataaaaac agatcctggg taagcaccag atctccaaaa
    29281 actttctcaa aaaatcccag tgcaatcgga tatacgaagg tacttacaac ggttgtcaga
    29341 gcaaattttc gtcccatgac cttccagccg atgagaagca tggttacatt aaatataagt
    29401 acaaaagttg ctaccggtat tccaaagctc ctgtttaatg ccagaccgat acctgtcgtc
    29461 ccccctgtaa gcaggcccgc aggcattaaa aacagcttta cagccagggc atacagtata
    29521 tttcccagta taacaatcat cagcgagccg cctttttgta aatggtcttt ccagttttct
    29581 gtatttgttt ccattttgtt ttcccctctc tcacccgtcc aatattcggg actttcatca
    29641 taaaaaaaca cgcctgtcag ggaattttcc ctaacagacg tggatcaagg cttctgccta
    29701 atacaatttt cagtataaag aacggaggtc attctgtcaa gcaggtttca ttgcttcatc
    29761 atttttccgt cagtttttct gttcctcata agttaaaaag gagtccatat actgaatata
    29821 ctcttctttt gtaaataccg gatgataatt cttaacgatc tcctttgcct ttacagcccc
    29881 gtttctaagc agtcggtatg cagacagggc gaagatcttg gcagtgacaa tataagccag
    29941 ttcttcatct acaatatcaa agtctactgt atgaagtcca tttacttttc cgcctgtatt
    30001 aaaagtaagc acaggatgga tgtgctgtac atctcctaca tccgtagaac cgccagaatg
    30061 atggctgatg tctttctcca ctacggtata ctccttttca ctggtggctg ctgcgatctt
    30121 agctgccgca aatacatctt catctgccgg tgtttttaaa gttggaagat atcctggggc
    30181 tgttgtgatc tggcaggtgg ctccaatggc agctgcacct gccttaaatg cacggtcggt
    30241 cttaaaggct gcgtctgcaa tggcgtcttt atttgaggca cgcaccaggg tttcgatcac
    30301 tacctcatcc ggaactacat tgaccagatt gccgcctttt gtaatgatgg gatggactct
    30361 tacatggtct tcatccttaa atgtttctct ttgataggct aaggctgaaa gaccaagaga
    30421 agccgcattt aaagcattaa ttcccttata tggcgcacct gctgcatggg cagccactcc
    30481 tttatagcgg atgaccttgg aaacaaaacc gttattagaa gtagcaccta cgcagatatc
    30541 ctcatcttct gtgtgatgta cgatatccag gtcaatatca tcaaaagcgc caatgcggat
    30601 caactcgcat tttccaccgc catagcggat ctttccctct tttgtcagct ggtttttaaa
    30661 ttcgatctcc ccatattcct ctgcaggaac agcaaaaaat accacctgac cgtctaaggt
    30721 ctccttaact ttcgaatctg taagagcgaa agccgccccg atcacacctg ccatctgggc
    30781 atgatgaccg cagcagtggg ctgcctgggt ttccggattt gcgtatttat gttccggaat
    30841 gcgaagagcg tctaattccc cgatcagcgc cagacttaca ttgtcctttt tctcttcgtt
    30901 taaatatgcc tttacgcctg taactgccag atgctctttt acagggagat ttaacttttt
    30961 catcaaccgg gtaaattttt ccgcagtctt aaattccttg tatccaagct ccgcatgtgt
    31021 atacatatct cttgcaaaag ctatgatctc atctctgttt gcctcgatca gatccacaat
    31081 acgtttctct gtttcgtcta actgtactcc cattttgcct tcctccttgt tttccccata
    31141 caacctgtcc atataatttc aggcgctccg aaactattgg tccgaaacgc ctgaaactgc
    31201 cttacagact gcgtacatcc gcagatctgt tacttatctg ttttcaatat ccattaacat
    31261 ctgaacacgg cgctcatgac gtccgccttc aaactttgca tccagccatg cttctgtgat
    31321 catcttagct aactcgctgc ctaccactct tgcgccaaat gcaaggacat tggagttgtt
    31381 gtgcattctg gaaagctttg cagtatatgg ctcgctgcat acgcaggcgc ggatgccttt
    31441 taccttgttt gctgctaaag agatacctac gccggtaccg cagatagcaa tacccagatc
    31501 agcttcaccg gaagcaactg cgcgtcctac tttttcaccg taaaccggat aatcgcagct
    31561 ttctgtgctg ttggttccaa ggtcgatcac ttcgtatccc ttgctctcaa caaattcctt
    31621 gatgatgttc ttcagctcaa tggctgtatg gtcatttccc attgcgatct tcataatact
    31681 aaatctcctc tttcttaaaa aactttttta ttttatctaa tctggatgtc atggacatca
    31741 aaagcagatc tgccactgta agagcagcca ttgcttccac tactaccaca gctcttggaa
    31801 cgatcaccgg gtcatggcgg cccttgatct cgatctcccg ctcttttcca tagcggtcta
    31861 ctgttttctg tatctgtgaa atagacggtg ttggcttaaa tgccgcccgc atcaccaacg
    31921 tacttccatc actcatgccc cctaaggtgc cgccggaatg attggtgact ttacatggtc
    31981 ttccgtcttt tcctataata aagcagtcgt tatcctgaga gcctaaagag gcggctgctt
    32041 caaagccatc tccgatttca aagcctttta cagcgccgat ggacataatg gcttttccca
    32101 gcgcagcatc cagcttatca aaaacaggtt ctcctacgcc tgcaggaagt ccgtttacaa
    32161 cacactcgat cacaccgccg caggagtctt tcctctgtat catatcttcc agataactct
    32221 ctgcctctgc tgccgctttt ttatccggca tatacaggcg gttattggaa atttcttcca
    32281 gatccatctt agaatggtcg atctttacag gtcccactgc actggtgtat gcctgaaccg
    32341 taattccaag gctttcaagg agctttgctg ctacagcacc tgctgccact cttccaatgg
    32401 tctctcttcc ggaagaacgt ccgccgcccc ggtaatcacg aaagccatac ttttcatcaa
    32461 aggtataatc tgcatggccc ggacggtata catccatgat attgctgtaa tcacgggagc
    32521 gctggtctgt gttgcggatc acaatagaga ttggtgttcc tgttgttttt ccctcaaaaa
    32581 caccggataa gatctctgcc ttatctcctt cgcttctggc tgtggtaaat ttgctctgtc
    32641 ccggttttct ccggtctaaa tatttctgga tatcttcttc atttaaagga agtcctgctg
    32701 gacagccgtc cactacaacg ccaacgcctg ccccatggga ctctccccag gtacttatct
    32761 taaaaatcgt tccgtatact gaacctgaca tgttattaat ctcctatttt tacaatggca
    32821 cagcagttag gttcatttac aggaatactt cttccactca taaccagaag gcctttttca
    32881 taatctactt taaagaaact tatggtgccg ctgtcatggt ttacagaagc aatatgtctt
    32941 ccatccggaa atacagctac atcctttgga tatgcgccgc tgatcggcag acagcatatc
    33001 atggtcagaa ggcctgtctc ctcatctctc ttgtaaacac taatggtatt ttctcctgca
    33061 ttggtgcaat atatgtattt ctggtcaaca gacattctca tggcgcaggc agcagtcagg
    33121 ttgtccggct tcttggtaga agtggtggaa atggtctgga tcttttcgat cacaggtgct
    33181 ctgtctccag tcttgtaggt gtatacatca attgctttct ttaattcata catcagataa
    33241 atgaattttc cgtctttgct gtaacggaaa tgtcttggcg cagactctaa ctcacagcgg
    33301 atggcgtcta cctggactaa tctctgttct ttatcattaa aacggtagat tttaacctgg
    33361 tcaataccat tatctacact gagtacaaag cggttgtcag gcattcttct ggtgcaggtc
    33421 acatgcgggc ggaagttacg ctctgccaca ctgccgtatc ctctgtcaaa tacaccgtct
    33481 acgatctttc ctacagaacc gtccgggttc agacttaata ccgtagtctt tccatcgtga
    33541 taaccggaaa cgaatacata tttatcctct tcatcggtac aaaggtggca gcctctcatt
    33601 ccacggatcg gggcactgtt taaacgtgcc aggcttccgt ttggaaggat acggaaagat
    33661 acaacgcctt catctgcaat ggaatacagt gtctttccgt cactggatgc aattacataa
    33721 gaagaattgt ccacttcgat ctcgcagcgc tctttaaaca ttccattttc tacatctaca
    33781 tcaaatacgg tgattccctt tgcttttccc gtataggaat aggaacccac atacgccata
    33841 tacttaccgc tcatacagct tacctccctg gatattgtct gtgtttttcc actcacggac
    33901 acgcaccaat gtgctctgtt gtctgctgct gcagacggtt ccgttcgcgc ttatttttcc
    33961 atgcgttcat cataccatac atttaattat ttgttaacct gtaagtttca gaaaaggtat
    34021 tgacactatt ctcatttaat gtataatgga actcggcttt gtttaataat actaaacttt
    34081 ttgcttattt ttatattcag ttagtatttg taaactttgt gtataagaaa tgttacttac
    34141 aggtaaaaca ggaggatatc atggatatcg gcaacaaact gaaagaactg cgtgttttaa
    34201 aaggactgac gcaggaggaa ctggcggacc gttccgagct ttccaaggga tttatttccc
    34261 agctggagcg caatcttacc tccccttcca tcaccaccct tatggacatc ctgcagtgcc
    34321 ttggtaccag tatcggagag tttttcaacg aggctcctga tgaacagatc gtattcggaa
    34381 aacaggacta ttttgttaag gaagacaccg aatacaaaaa cgagatcaaa tggatcattc
    34441 ccaatgccca gaaaaatacc atagagccca tttacctgac cttacaggcc ggtggctcta
    34501 cctgtccgga cacacctcat gaaggagaag aatttggcta cgtccttcag ggagctgtgt
    34561 ctatccatct gggaaataag acttataaag ccaaaaaagg ggaatccttt tattatacag
    34621 ctgacaaaac ccatttcctg tccagtaaaa acggggcaac cctgatctgg gtcagctcac
    34681 caccaagctt ttagtatgtc ctcccgaaac tttgggagaa cggatcacca cacctacgaa
    34741 aaaggagaac tgcgcacatg agccagccat tgattgattt acagcatatt tcaaaaagct
    34801 ttgacgggga aatggtctta gatgacctga acctttctat aaaagaaaac agcttcgtga
    34861 ccttactggg tcccagcggc tgcggaaaat ccactactct gcgtatttta ggcggtttta
    34921 ccccgcctga taagggaaac gttatttttg acggtcagga cattaccaag ctgccgccaa
    34981 acaagcgcca gttaaacact gtattccaga aatacgctct gttcccacat atgaccattg
    35041 cggaaaacat tgctttcggc ttaaagatca aaaacaagcc aaagagctac attgacgata
    35101 agatcaaata tgccttaaag ctggtaaacc tgtccggttt tgaaaaaagg gatgttatct
    35161 ctctttccgg cggtcagcag cagcgtatcg ccattgcaag agccattgta aatgagccgc
    35221 gtgtcctgct ccttgacgag cctttaggcg ctctggacct gaagctgcgc caggacatgc
    35281 agtatgagct gatccgactg aaaaatgagt taggcattac ctttatctat gttacccatg
    35341 accaggaaga agcacttact atgtctgata ccatcgtagt tatgaaccag ggctatatcc
    35401 agcagatggg ttccccggag cagatctaca atgagccgga aaatgctttc gttgccgact
    35461 ttatcggtga gagcaacatt gtacctggcc ttatgatcca tgatgaactg gtcgagatct
    35521 tcggagcaaa attcgcctgt gttgacaagg gctttggaaa caacaaacct gtagacgttg
    35581 tgatccgccc tgaggatatt gacctgttaa agccggaaga aggcacactt cagggtgttg
    35641 tcacccacct gatctttaag ggcgttcact atgaaatgga agtgactacc ccggatggtt
    35701 ttgagtggct ggtacactcc accgatatgt tcccagttgg ccagcaggta ggacttcatg
    35761 tagatccttt cgacattcag atcatgaata agcctgcttc tgaagatgag gaggcactgg
    35821 gagtcaatga ataatgataa aaaaaaatcc gtggctattg gacgggcact gctctccggt
    35881 ccttatctga tctggatgat cggttttacc atcattccgc tggcacttat tttctggtac
    35941 ggcattacaa ctaaaaacgg taacctgaca ctggcaaaca tcacagctat catgagcccg
    36001 gatcactgga aagccttatg gctgtccctt ggactgtctc ttatcagtac agtcctctgt
    36061 ctggtcctgg catatcctct tgccatgatc cttcgcggaa gaggcagctc tgccagcggc
    36121 tttattgtat ttatcttcat ccttcctatg tggatgaact tcctgctgcg cactatggca
    36181 tggcagacat tacttgaaag aaatggtgtg atcaacggta tcctggcatg gcttcacctg
    36241 ccaaagcaga acctgatcaa caccccggca gccattgtcc ttggaatggt atacaacttc
    36301 cttccattca tggtactgcc tatctacaat gtacttgcaa aaattgacga caacaccatc
    36361 aatgccgcaa aggacttagg tgccaatact ttacagactc tgttctttat ctggctgcct
    36421 ttaagccttc caggtattat cagcggtatt accatggtat tcgtgccttc ccttaccaca
    36481 ttcgttatct ccgacctgtt aggcggaagc aagatcctgc tgatcggtaa tgtaattgaa
    36541 caggagttta caaagggaaa taactggcac ttgggaagcg gtctgtctct ggtcttaatg
    36601 gtatttatcc tgatcagcat ggccatgatc gcaaaatacg ataaaaacgg agaagggacg
    36661 gcattctgat gacaaaaggc aaagaacggt ttcggaagtt tatttccgac ttttatttag
    36721 tgattatcct tatcttcctt tatgccccga tttttactat gatggtcctt tcttttaatc
    36781 agtcaaaatc aaggacccac tggggcggct ttactttaag ctggtataca cagatgtttg
    36841 aaagccgggc gatcatgtat gcattataca cgactctgtt agtagcaatg atctccgcac
    36901 tggtggcaac tgttattgga actgtaacag cgatcgctat cagccatatg aaacctgttt
    36961 ccaaaaatat ttttatgagc atctccaaca ttcctatgtt aaatgcagat atcgtaacag
    37021 gtatcgcact gatgctggca tttatcgctt ttggcatttc cctgggattt aagacagttc
    37081 ttatttccca tatcacattt aacatccctt atgttatttt aagtgttatg ccaaaattaa
    37141 agcagacaga ccgctctact tatgaagcag ctttagacct tggcgcttct ccggcttatg
    37201 ccttttttga ggtagttttc ccggatatca tgccaggtgt actttccggt ttccttctgg
    37261 catttaccat gtcactggat gatttcatta tcacccactt tacaaagggc gccggcatca
    37321 atactctgtc taccctgatc tacagtgaag tacgccgcgg tatccgtcct tccatgtatg
    37381 ccttaagcac tgttatcttt gtggtggtgc tggtggtact gcttattgca aatttccgta
    37441 aacagccgga aaaagactaa tatcagagac tggaggatct aagctatcat gaaaatgcgt
    37501 acattctggg ctgctgctgc agctgctgta ggctcctgcg tggtatcgat cgctatttta
    37561 ggagttcccg gggcagaaag cggcggcggt accttaaaag tatataactg gggcgaatat
    37621 attgacgagg atgttattac ccagtttgaa gatgaaaccg gtatttctgt tgtctatgac
    37681 gtttttgaaa caaacgaaga aatgtatcct gttgtggaag caggcggcgt taaatatgat
    37741 gtagtatgtc catctgacta catgatccaa cgaatgatcg acaacaatat gttagccgaa
    37801 ctggattatg ataatatccc caacgcaaag cagatcgggg atgattactg gaacatgagt
    37861 aaggattttg acccgaaaaa ccagtatgca gttccttact gctggggtac tgtaggtatt
    37921 ctttataata agcagatgtt agagcagtta ggggttccga agccaaccag ctggactaat
    37981 ctgtgggatc cgaaactttc tgatgagatc ctgatgcaga acagtatccg cgatgcattt
    38041 atgatcgcat taaagcagaa aggctattct ttaaatactt ccaacccgga agaactagct
    38101 gaagcaaggg atatgctgat ccagcagaaa cctctggtac aggcttatgt tattgaccag
    38161 gtaagagata aaatgatcaa cggtgaagct gcagaaggcg ttatttactc tggcgaaatg
    38221 ctttacatcc aggaacagat tgaagaactt ggtctggatt atgacctgga atatgtagtt
    38281 ccggaagagg gaaccactct ctggatcgac tgctgggttg ttccgaaaaa tgcagaaaat
    38341 aaagaagcgg ctgaaaaatg gatcaatttc ttatgccgtc ctgatattgc aaagaagaat
    38401 tttgactaca tcacctattc tactccaaat acaggcgcat atgagctttc tgatgaagaa
    38461 ctgcagaata atgaagccct gttcccggat atggactctc cggcaatgaa aaacagtgag
    38521 atcctgcgct atcttggtga tgatacggat aatatttata atgaaatgtg gaaagaagtt
    38581 aaggcacagt aactttcatg ccaaaattta tttttcaggg aattttatca ataaggattt
    38641 attgcaattt ccattacttg taaatatgat ttttctatga taaaattatt gtaattagtg
    38701 ggagcgtgtc tgaaaatttt tgtcacgatt ttcggatgcg ctctacggct actttgagtt
    38761 ctaatgaaag ggcgtgttgt tgtgaaaata caggaatctg ctgaaaatta tctggagacc
    38821 atacatatgc tcagcaagca taaaccttat gtgcgttcca ttgatattgc aaatgaatta
    38881 agcttttcca aaccaagtgt cagcgttgcc atgaagaacc tgcgtgaaaa tggctatatc
    38941 cacatggatg accagggcca catcactctg acccagactg gtcaggatat tgctgataag
    39001 atgtatgagc gtcatattat gctgtctaac tggctgatct acctgggcgt ggacgaaaaa
    39061 accgctgtgg aagatgcctg caagatcgag catgttctca gctcccagag tttccaggct
    39121 attaagaaac atattactca gggaaatgag cttcctgctg atatgaagga agaagagtaa
    39181 tcagcaaagg cgccaccggc gcctctttga tatgccaaac aataaatttt tgagctatga
    39241 aagagggatt gttatgttaa aggatcttgt gaagaaatgt cgttcttacc gccgtttttt
    39301 tcaggaggtt tctattcctt atgaagacct gaaggatatg gtggatacag cccgtctgac
    39361 cggttgtaca aagaactgcc aggggcttaa gtttaagctg atctctacac ctgaagagtg
    39421 tgcaaaagta ttcccgtctt tgttatgggc cggtgcctta aaggactggg acggacctgc
    39481 agatggagaa cgtccaagtg cttatgtgct ggtacttgag gatctttcta ttggaaaaaa
    39541 tctgatgtgg gatgagggca ttgtttccca gacgatcatg ctttctgctg ttgagaaggg
    39601 atatggcggc tgtatgatcg gaagctgcaa acgtgttgag atcttagaag ctgttgagct
    39661 tgatccacag cgttatagtc ttgcgctggt gctggctctt ggtaagccga aggaagaagt
    39721 tcgtatggtg ccggttggcg aggatggcag tacaacttat taccgggatg agaatcaggt
    39781 gcattatgtg ccgaaacggg cgttggaaga tattctggtt taggatgttt gaaaatttag
    39841 gcggtcacag ggcgtggccg tctttttgca ttttgggaag gagatttctg atatgatggt
    39901 taagcatatt tctattgaag ttacttctaa acagactaaa aagcctgttt tcggtggtcc
    39961 ggcggatctt actgcttata ttttggaacc ggtggatggt attctggata aaaagaggcc
    40021 ggctgtgctg ctttgcccag gcggcggata ccgtacactt tctaccagag aagaccagcc
    40081 catcgctatg aagtatctgg ctgctggttt tcatgttttt gtgcttcatt acagtctggc
    40141 gccggatgtg tttcctcggg ctttgatgga gctggctctt gctatgaagg tgatacggga
    40201 gcatggggat gagtggaatg tggatgtgga caggatcgct gtttcgggct tttctgcagg
    40261 cgggcatctg gcatgctgtc tgggcgtgtt ctgggatagg gaatggttgt atggggcttt
    40321 gggggtaaag ccggaaatga tacggccgga tgggatgatc ctttgttatc cggttattac
    40381 ttctggagaa tattgtcata aagggtcatt tgaatgtctt atgggagctg aggcttctaa
    40441 gaaggatgaa aaacttcgca ggcttctgtc cttagagtat caggtgggtc ctcaggttcc
    40501 aaaaactttt ttatggcata cctggacgga ccagtcagtg ccggtagaga acagcctgtt
    40561 gcttttacag gctttaagaa aggccggagt aaatgtggag gcgcatttgt atccggtggg
    40621 gcctcatggg atcgcgcttg ggacagaaga aacccaggga atcgatcaga aatatctgga
    40681 gccttattgt gatagttgga tggggctagc gatacgctgg attgagaagc agctgtgaga
    40741 atgtgaatgc tgagaaattg gcagtgtgag gttttaagtt gtgaagaaaa cggcctaaca
    40801 tttcacttat cattgtccac tggctttcga catgcacctg ttcgcctgca tcggactacg
    40861 tccgctgcat tcggacagct gcgccgggga tgtggacaat cgataagtga aatacggcct
    40921 gtgtatacaa ggagtaaaaa ttaaagaagc tttttattcc atagagtttt ggatgatgtt
    40981 taatattgtt tccagatcaa tgccttcatc aacagaaagg gagtatgaaa agtcttgtgt
    41041 tgtccaggtt gcaagataat atttatcatg attgcctttt attgtaatat caacaccgtt
    41101 tacaacttta tccaatatct gttcatactg gttgtaatca ccggagatgt cgtctgttcc
    41161 acttgttttt ctgtaagtca gggaattatc ggtcccctca tatatgatct gggcaaattc
    41221 gttccagcac cagaggtaag aagtagacat aactttaaat ggcatatctg cttcagagat
    41281 gtcttttaca ttgaaaccgg cttttttggt cagttctgca agggacttac attcttccat
    41341 accgtatacg cttcctgcca gttctggatt gggatcggtg gatgatgggt aaaaggcatt
    41401 tttcatcatg gttccgccta tgagcaaaag tgcggtgcag gcagcaatgg atgccatttt
    41461 ccgcaagtgt gggaatgtga ccaccttgtg cgaagtattg gatacttgtc tggatacatt
    41521 attcagtata tgagtacgca tttcatcggt aacttctatt ttttccatga tctcatgata
    41581 agatttactc aaaatcatac gcctccttta atacttgttt taaggcttct ctgcctcgtt
    41641 tcagatcgga ccggatgctg ctttcgttgc ggttcaggat cttactgatc tctttggtgg
    41701 gataaccttc ctgatagtag aggtgaagca cttctctgta tttttctggc aatgctttta
    41761 ctgcttccca tacaaaggac agatcttctc tttcttctgc caccaatgtt tcttccagtt
    41821 cgtctgtttc ccggatcttg ttataatcta ttttgttttt gcttaaatta gctgctacat
    41881 gaagaagcca tgctttttta tggctttcgg taaggaacgc aggagatgat tgcatatatt
    41941 tgatcagggt atcctgcagg atgtcctcgg catcactcat gttatggaga taggagtatg
    42001 caagccgcag gattgcatta ccatagatat ccaatactgt ttctatagct gatgtatttt
    42061 gcaaaacacc cacaggcgcc gtttctatct ttcttgccct ggttgacaat atggctgcaa
    42121 acagatatgc aataccatta agcacattat ctaccaatgt atgccatatg gacgtgctgt
    42181 ttatttccgg taaaatactg atgttgctga tactattcat cgtttggttc ctttatcctc
    42241 atcactctat ctgctggatg atattttcca tttcactctc actcagtccc ggagtgctta
    42301 cagagtacgt atagcctgca tctgtccatg ttgccagcat aacttttccg tcattgccct
    42361 gttccattat ctggttttta tttatatcca actgtgtttt ctcgttgtat gtgttgtaat
    42421 caccgctgat gtcatcagaa ccaattcctt ttctaataag aatagtgcta tctgactgat
    42481 cgtctttttc gtaaaatacc tggatgactt ctttgtctaa tgtctgaatc atggtccgtg
    42541 tataaccatc gatcttttct ggaactgtga ttgaaaatcc aacattatct tctgcctcgt
    42601 tcagattttt acagtctgtc catggatttg gcatacccac agtttcacta accgtttctt
    42661 gttcttctgc tgttttggtt tctgttactg cttttgttac ctctggcgtt acgggttctt
    42721 tggctccaca tgctgccata gaagtcattg ttgttgcaca cattaaaact aacattaatt
    42781 ttctcatttt tataagtcct ttcgtatcta taaaatatga tgttggggtc aaaaggtatt
    42841 ttgacccctc tgataccgaa ttgctacgtg ctttgcactc tcgcaattct aaaaacattc
    42901 gtaatccgct catttttct
//
LOCUS       DBMDUA010000004        40367 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_840_length_40367_cov_4.201560, whole genome shotgun sequence.
ACCESSION   DBMDUA010000004 DBMDUA010000000
VERSION     DBMDUA010000004.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 40367)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 40367)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40367
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_840_length_40367_cov_4.201560"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2008
                     /locus_tag="ACZPCL_00640"
     CDS             <1..2008
                     /locus_tag="ACZPCL_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1161036.1"
                     /translation="LQWPIFLSVLMVLLTAAVGAVSLRAGIIVSFFTFIYIGIALWIY
                     FSRRKGILAGLIAFSQVYDKSKSKFLEEMLVPYCVADIAGRILWMNQEMEAILGDDKA
                     TIKNITQMFPDVTKEMLATGGETVSIHSSFKDKKYRVDMKEIHTEEAQAAAADCGLDE
                     GNSLVTAIYLIDETQMLLYKQQINDQKLVAGLIYLDNYDEALESVEEVRRSLLTALID
                     RKISKYISSMNGIVKSIEKDKYFFVIKQQYVAKMQDERFSILEDVKTVNIGNDMAVTL
                     SIGIGMNGESYAQNYDYARTSIDMALGRGGDQAVVKDSDKILYYGGKAQQMEKTTRVK
                     ARVKAHALKELMDNKDRLLIMGHRMADIDAFGAAVGIYRIAMSMNKKANIVLNDVTSS
                     VRPMMERFQNNPEYPDDMFLNGAKAAELVDAGTMLVVVDVNRPSITDEPSLLRLIRTI
                     VVLDHHRTSSEVIDNAVLSYVEPYASSACEMVAEVLQYIADGIKVRPCEADAMYAGIV
                     IDTQNFTNQTGVRTFEAAAFLRRSGADVTRVRKLFRENMVDYQAKAEAVRAAEVYMDA
                     FAISVCPAEGLDSPTIIGAQAANELLEIKGIKASVVLTDYNNTIYFSARSIDEVNVQV
                     MMEKLGGGGHRTIAGAQMQGVTIEEAKERLKAVIRQMMDEGDVS"
     gene            2083..2529
                     /gene="rplI"
                     /locus_tag="ACZPCL_00645"
     CDS             2083..2529
                     /gene="rplI"
                     /locus_tag="ACZPCL_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR1161037.1"
                     /translation="MEIVLLEDVKALGKKGQVVKVNDGYARNFILPKKLGVEATSKNL
                     NDLKLQKANDAKIAAEQLAAAKELAEKLNNASVTVSIKAGEGGRAFGAVSSKEIEKAA
                     MDQLGLEIDKKKIVLNTPIKAIGSFEVSVKLHKEVTAKLAVKVVEA"
     gene            2579..3937
                     /gene="dnaB"
                     /locus_tag="ACZPCL_00650"
     CDS             2579..3937
                     /gene="dnaB"
                     /locus_tag="ACZPCL_00650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR1161038.1"
                     /translation="MEETLIKRVLPHSIEAEQSVIGSMLMDREAVIAASEIITGSDFY
                     QQQYGIMFDAMVELFNEGKPVDLVTLQDRLKEKDVPPEVSSLDFVRDIITIVPTSANV
                     KSYATIVSEKAVLRRLIKTTEEIANTCYAGKEPLENILADTEKSIFDLLQNKGGQEFV
                     PIKQVAINVLEKIEDAYKNQGTVTGIPSGFIDLDYKLSGFQPSDFILIAARPSMGKTA
                     FVLNVVDYVSVRKNLPCMVFSLEMSKEQLVNRMLSMESNVDSQKLRTGTLTDSDWDAV
                     VEGIGIIGNSKLIIDDTPGISISELRSKCRKMKLEYGLSIVIIDYLQLMSGSRKGGND
                     NRQQEISDISRSLKALARELHAPVIALSQLSRACETRTDHRPMLSDLRESGAIEQDAD
                     VVMFLYRDDYYNKDTEHPNEAEVIIAKQRNGPIGTVNLIWKPEYTKFVNAVRPQGGNQ
                     EG"
     gene            4089..4772
                     /locus_tag="ACZPCL_00655"
     CDS             4089..4772
                     /locus_tag="ACZPCL_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1161039.1"
                     /translation="MDEVHYLISDASRKVDVEAHVLRYWEEELELAIPRNEMGHRYYT
                     DFHIRLFKQVKNLKEKGYQLKAIKHALEQIKKDGKLEGELTDEILEQDMDAALKELKE
                     EEKEENTAMVSTSADGSPLPVSAEEKMAQFQQMMNLIIGRALEVNNEKLSQDISCLVK
                     QRLAGELELLLQESGQKEEERFRLLDETIRSFQKAGQEEAAATKVPFFKRKRFGRGAK
                     KLFHREKRS"
     gene            5039..6649
                     /locus_tag="ACZPCL_00660"
     CDS             5039..6649
                     /locus_tag="ACZPCL_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR1161040.1"
                     /translation="MNEKYTYYLRYACISSALAAALALGGCSSGERGVATQNEAVAAN
                     AQDGQAAVSDGTDETIAPLGMDGQILPGLDDVELPEDAQAAPQYLREGVRHEIVKKLQ
                     QRLMDLGFMDNDEPTDYFGQVTLTAVKHFQRQNELPQDGIVGDTTWNELMADDAKHYA
                     VSKGTQGDDIQKIQQRLYELGYLASADQVTGNFDDATETAVLKLQGVNGLAEDGKVGQ
                     QTYNLMYSDDIKANMLAYGEKSDVVLACQQRLKDLGYLTTTPDGTYGQDTVIAVKQFQ
                     ARNDQVVDGYLGPSTRIVLNSSDAKPNGLMIGEQGDSVTRVQQLLSKYGYLPSSNVTG
                     YYGEATEDAVKNFQSRNGLSADGKVGVQTMAKLTSDNVKKPAPTTAASTKSNKSNSSS
                     NKNNSSSKGNSGGNTSSPAPRPASGAGVSALISVASSKLGSPYVWGAKGPSSFDCSGF
                     VYWCLKQVGVSQSYMTSSGWRSAGRYTKVSNFNDIQAGDIVVVRGHVGIAAGGGTVID
                     ASSSNGKVVHRGLSSWWRSNFVCAWRIF"
     gene            6756..7451
                     /locus_tag="ACZPCL_00665"
     CDS             6756..7451
                     /locus_tag="ACZPCL_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1161041.1"
                     /translation="MQTILVCDDDKQIVEAIDIYLTGEGFKVIKTYDGYEALEYLETQ
                     SADLLILDVMMPGLDGIRTTLKVRETSSIPIIILSAKSEDADKILGLNIGADDYITKP
                     FNPLELVARVKSQLRRYTQLGNMNQQAEGQVYKCGGLQINDDNKEVTVDGDPIKLTPI
                     EYNILLLLVKNAGKVFSIDEIYEKIWNEEAIGADNTVAVHIRHIREKIEINPREPKYL
                     KVVWGVGYKIEKQ"
     gene            7459..9969
                     /locus_tag="ACZPCL_00670"
     CDS             7459..9969
                     /locus_tag="ACZPCL_00670"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1161042.1"
                     /translation="MEKRAFGLRKWKVLLSVFHVIFLVITLAGISAMYLNGGYGKGIR
                     WIYDETYEDSDAFCSQLGIDISNIFTYIGYKDMFETNGVLDMHKPIVRIIDGPGVDED
                     MTLDQIVRYAKIRGYYLGEDFTVQGSPMAMDDDDMEITVEYKSAKTDLADVDGLETRM
                     TKEEMALDILDRLGKYYTIYYNFISNNTNVRFRILYKSNDGREVVYTNVPSMSLDELR
                     GFGKYLFIPGNTIRMESNLATIPDNAASLLETWNPFDNDQNYMVVSVDTTYPYSDNYA
                     EEAGEYKSARSTFIMGMGGVIVGIAGFFATLVTLVLLSGHEDGADGKIRLFPIDRIYT
                     ETCVLLWAVVTAVILYFCKRLGMMVIGLFASEEHLAYWSKLLKYVIIYGCSILCGFSL
                     LRRYKADTLWKKSIAKKVVKASKDYIAKASFVAGIGLCYLLFLGANGVMLWGILFLFA
                     YIDEKLTFRILFYVCAVLFVGLDGWVFHKLFRKAEQKDLLNNAITCIAKGDTGFRLDT
                     KKLTGKERTVGDHINNIGSGLDTALQEKVKSERLKADLITNVSHDIKTPLTSIINYVD
                     LLKRQKIQDPKIASYLEVLDQKSQRLKTLTEDLVEASKASSGNLKLEMADIDLVELVQ
                     QTNGEFEERFEQRHLKIISDFPDGMIIIRADGRRLWRVLENLYTNAFKYAQEGSRVYV
                     DVASVDGKAIFTMKNISEKPLNISPDELTERFVRGDVARTTEGSGLGLSIARSLTQLQ
                     KGEFVITIDGDLFKAQVIFPQVRQETRAEMRLERAAEEKQAEEQSGEKMSGEMPVGEN
                     LLESAPYNWDVMVENDKNLTAKEEILIQNGKREHKPEK"
     gene            complement(10039..10641)
                     /locus_tag="ACZPCL_00675"
     CDS             complement(10039..10641)
                     /locus_tag="ACZPCL_00675"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR1161043.1"
                     /translation="MEQNNSSDLLLVIDMQNVYTKGQEWACKGIEHASASILRLLNSQ
                     KTKQVIFTQYLATEHPDGVWKEYNKVNAAVNSDPWLNEMMPEFLPWVKTYPVFTKSVY
                     SSFAIPEVVKAAKKARHLVISGVVAECCVLSTVLSAIDAGCKVIYLTDAVAGLSEVSR
                     KETENIISYFAPLHTVLMTTEEYLLSDTPKSHLCYKVSGH"
     gene            10920..11654
                     /locus_tag="ACZPCL_00680"
     CDS             10920..11654
                     /locus_tag="ACZPCL_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HIR1161044.1"
                     /translation="MGENKMPHIQLPENLGIRYAVLPGDPARAERAAGYLKDAKLLTF
                     NREYKSYSGFWNGVPVLVMSTGMGGPSMAIAVEELQKIGVEAAVRIGSCGALQPEVRV
                     GDLVMVEAAVRDEGTSLGYAPLSYPAVADHRLLTACEESAKEAGCRFHTGIARSHDCL
                     YRDDNPVIYEEWAKKGVLASDMETAALFVVGRIRGMRTASILNVVAACKGNVAENIGR
                     YVDGETLTAQGEEYEILTAFAALTRQ"
     gene            11713..12315
                     /locus_tag="ACZPCL_00685"
     CDS             11713..12315
                     /locus_tag="ACZPCL_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1161045.1"
                     /translation="MKNLFKTKFDVAVIVLIPACIGINYLGKLFASVLKLPLWLDSIG
                     TCIGGCLGGPIIGAICGAANNLIYGFSAGDNITLIYALTSLGIGAAAGIMARLGFMKS
                     FPKAIITACVAGFVAVAISTPLNVLFWGGTTGNVWGDAVFAATQAANMPVFLGSLLDE
                     VVVDVPDKLITLIMVFFILKGLPNKLTTLYEVNSEIETLD"
     gene            12341..13114
                     /locus_tag="ACZPCL_00690"
     CDS             12341..13114
                     /locus_tag="ACZPCL_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR1161046.1"
                     /translation="MKSISLFVDNGTYLTKVHPFTKLMYIAAAISIPLISGRLWMFGV
                     FIAVSLLVLASGKILKKTLPLIAFSFTILLTIFLIHGLFNQKNVNVLFQVVGLKFYRE
                     GIFYALHIGLNILNMLLSFAVFVLSTKPSEFVDELEKRGFSPRFGYIVTSVFQIIPQM
                     MGTMNTIMDAQRSRGLETEGNLRTRGKAFLPLISPVVMSSLINTRERAIALEVRGFGA
                     GKKKTWLTERTRHKGDREITIFLGVCILAAVVWRIVGCL"
     gene            13105..14919
                     /locus_tag="ACZPCL_00695"
     CDS             13105..14919
                     /locus_tag="ACZPCL_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1161047.1"
                     /translation="MSVITVENLRYRYPHTKELALDGLDFSVEKGEFIGIIGENGAGK
                     STLSQAIMGLVPQFYKGAYGGMVTVDGIEAGKTPVAQLCGHVGLVFQNPFNQLSGAKD
                     NVYEEVAFGMQNLGVPAEEMKKRVEEALKLLDIWQYRDRNPFDLSGGQMQRVAIASVL
                     VMRPDVMILDEPTSQLDPEGSDEVFRAVETLTGSGITILMIEQKIEKLAAYCDRILLL
                     HKGKQIAFDTPQKVFSMPDLNDYGIQAPAFTRICKAEQVTLADGTYPVTVKEAAEVLR
                     EKRLGVYEHANAEAGVQKAVGASAAGTGQVQAKNAETGARSGQSGTCRKAENVASVDE
                     QFRIEKLDFSYLADIPVLEDLNMKLDQRPTAIIGQNGAGKTTLVKLLKGLLKPVSGSI
                     YFHGEDISGKTVAMLAGSVGYVFQNPDDQIFKYNVMDEVLFGPLNIGMDPEQAKKEAA
                     WALELTGLSGKEKENPYDLELYERKMTAIASVLAMDTDVLILDEPTIAQDWKGRQIIG
                     SIIRSLSGRGKLVIAILHDMDFVAENFERVIIMAHGQVLADGTAKEVFAQEEVLKKAR
                     LQKPYVMQLSEALGYEKSYLTVEELLKDRMSLCAAIKL"
     gene            complement(15119..15868)
                     /locus_tag="ACZPCL_00700"
     CDS             complement(15119..15868)
                     /locus_tag="ACZPCL_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR1161048.1"
                     /translation="MAKEMIFSSAKKTVYRDEDTAIKEFCEGFPKAEVLNEALCNARV
                     ETIEALNVPKILGVTVTDGKWSIIKEYVPGKTLEQLMDENPDKLDEYMSQMVDLQLLI
                     HSQACPLLNKLKEKTIRSLKSVEQLDESTRYELLTRLDGMPKHTKLCHGDFNPSNIIV
                     GDDGKLYVIDWVHASQGNASADVARTYLLLSLKDKKKAELYMDLFCEKTGTEKRYVQG
                     WLPIVAAAQLAYKRPEEKDLLESWINVFEYQ"
     gene            complement(16104..18107)
                     /gene="glgB"
                     /locus_tag="ACZPCL_00705"
     CDS             complement(16104..18107)
                     /gene="glgB"
                     /locus_tag="ACZPCL_00705"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR1161049.1"
                     /translation="MRKQAGQTKKNTTTAAHKTTRRSAGTKTAAKSTPSKKASANTGL
                     ISDLDCYLFGAGTHYEIYKKLGAHPMTYKGKKGIYFGVWAPHAQAVHLVGDFNGWNPE
                     ANPMTKVGQSGIWEYFNAGMQVGELYKFAITTDTGKILYKADPFAFSAEYRPGTASVT
                     ADISGFSWNDSDWISKRSQADMTKQPISIYEVHLGSWKKKDREEKDGYYTYVEAAHEL
                     AAYVKEMGYTHVELMGIAEHPYDGSWGYQVTGYFAPTSRYGSPKDFMYFVNYLHKNGI
                     GVILDWVPAHFPKDAHGLADFDGHPLFEYEDPRKGEHPDWGTKVFDYGKNEVKDFLIS
                     NALYWVEEYHVDGLRVDAVASMLYLDYGREPGQWVPNKNGGNQNLEAIEFFKHLNTVV
                     QGRNHGVAMIAEESTAWPKVTDHPENDGLGFTFKWNMGWMHDFLEYMKLDPYFRKYNH
                     NRMTFSLTYFTSENYILVLSHDEVVHLKCSMINKMPGLGEDKFSNLKAGYTFMLGHPG
                     KKLLFMGQDFGQWHEWDEKVSLDWYLAEEESHKKLQEYVRDLLHIYKKYPCLYQLDND
                     WNGFQWINANDGDRSIFSFIRKDETGKKNLLFVVNFTPVDRPDYRVGVPKRCKLSLLL
                     DNVHGAYKPSQCPTYTSVKSECDGQPYSISYPLGGYGTAIFRF"
     gene            complement(18115..19473)
                     /locus_tag="ACZPCL_00710"
     CDS             complement(18115..19473)
                     /locus_tag="ACZPCL_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR1161050.1"
                     /translation="MSTWYEQAIFYHMYPLGITGAPKINVQTEVTDRFKELDQWIPHI
                     RSLGCNAIYIGPLFESSSHGYDTRDYKLVDRRLGTNDDFREFTKLCHENGIKVVVDGV
                     FNHTGREFFAFKDIQEKKWDSPYKDWYKGVNFGWQSPLGDPFGYEAWQGHFELPCLNL
                     YNQQVKDYLFDVIRFWVDEFDIDGIRLDCANILDFNFMKEMRAKTASMKPDFWLMGEV
                     IHGDYSRWVNPEMLHSVTNYELHKSIYSGFNDHNFFEIAHNVRRLEAIGRQLYTFLDN
                     HDEDRIASKLKVKEHLYPVYTCLMTLPGIPSIYYGGEWGTEGKRTSTCDDDLRPCIPA
                     EKIPELNCELTDFISRLGKIHEENEELHTGKYQELLLTNRQYAYARWGNNSLIITALN
                     NDDNEATLNIPVPIQAQTAVDLLNSDNETCENNVLPVQNGRLEIKLKGNWGAVLKIKG
                     EN"
     gene            complement(19478..19723)
                     /locus_tag="ACZPCL_00715"
     CDS             complement(19478..19723)
                     /locus_tag="ACZPCL_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2508 family protein"
                     /protein_id="HIR1161051.1"
                     /translation="MPGFLKAIREAAAAAPVNQDLVFTRRKLWDDIERTLWALETAQN
                     HFEQATEPSLIDCYIYEWNAAQLRYQFLLRKFKNMED"
     gene            complement(19803..20153)
                     /locus_tag="ACZPCL_00720"
     CDS             complement(19803..20153)
                     /locus_tag="ACZPCL_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR1161052.1"
                     /translation="MTTVQDLQKQFHCCMPLFIALGDKVRLSIVELLTEAAVRSEDPQ
                     GTIDFEGHAMNVNEITRRTSLSRPAISHHLKILKDAGLVGVRQDGTANYYYLTLRDST
                     RSLMELGYRLEEFM"
     gene            20410..20991
                     /locus_tag="ACZPCL_00725"
     CDS             20410..20991
                     /locus_tag="ACZPCL_00725"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HIR1161053.1"
                     /translation="MRNSVYGSLSYLSQSVFWGTLLLAAWSDWKYYRIPNRLIIFGIV
                     SGIMSLVFQAADLEAGSKIPWLLLALSGFLVRGVAVLVLGIFLHSLGMIGAADLKLVA
                     LLAAWLGFSETIKILCVGFILGAVWSLQKMLRYGSAFQRFLYLSAYIRQILSNKKIEK
                     YYDPVRDGTECVIPLGTCFCMATVLVFLEKWIF"
     gene            21003..21992
                     /locus_tag="ACZPCL_00730"
     CDS             21003..21992
                     /locus_tag="ACZPCL_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR1161054.1"
                     /translation="MIKVMVVYDEDPLYAGRLAEYVNQKETFPFQAMAFSDLEKLKAY
                     GRDHEIAILLVGERVREEAKEIKAGLKMLLCDGEFVSQEEASVYKFQSGDCILQEVMA
                     CYCTVPPEPGLALIGKRALIMGVYSPIGRCGKTSFSLTLAHMLGKSQGVLFISLEEYS
                     GFSKLVCGGYEQDLSDVFYLYRQGDFNWLKLKSLILSHGNVDYIPPAAYGEDLDQAQP
                     EEIAGLLKQIGTESGYERIVVDMGHMGKGSLELFAACDVVYMPVLDDPVSTAKLEDFE
                     TYLKEAGHKGMEEKIQKLHLPEIKTGIRRGGYMEQLLWSEMGDYVRKLLEGGY"
     gene            22065..23210
                     /locus_tag="ACZPCL_00735"
     CDS             22065..23210
                     /locus_tag="ACZPCL_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HIR1161055.1"
                     /translation="MGQLKDQIYLEDEQLYEQIDTAIRQKGKETYLPLKERLWLRNSL
                     YDSFRRLDILQELLDDKDVTEIMINGAGKIFIEKKGNMVLWNRRFEKTEQLEDIIQQI
                     VGKVNRIVNVSSPIADARLEDGSRVHIVLPPVALDGPVVTIRKFPDPITIEKLIRFQA
                     LTEEAAQFLEQLVKAKYNLFVCGGTNSGKTTFLNALSSFIPETERVITIEDSAELQIT
                     HVPNLVRLETRNANTEGEGEITISQLIKASLRMNPSRIIVGEVRGAEALDMLQAMNTG
                     HPGSLSTGHGNSPQDMLSRLEVMVLMGAELPLDAIRSQIASALDIMVHLGRLKDGSRK
                     VLSIVEIGGYKNGKVEQKPLFVYDREEKRLKKTGELKAREKLADAGD"
     gene            23197..24018
                     /locus_tag="ACZPCL_00740"
     CDS             23197..24018
                     /locus_tag="ACZPCL_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR1161056.1"
                     /translation="MQEINRKRWSRSSGREKQKEDLNGRARPPDYREYQLSQKEWMQY
                     LIMGAGAAGLCAYTFYRSVLAFLFLLPVAFLYPVYKKKDLKKERIRRLTLQFKEGIMV
                     LASFLSAGYSLENSLSLSVKELEGLYGKTGMITEEFSYMEAGVRMNRPVELLLADMGR
                     RSGIQDVDQFARVFAAAKRSGGELTDIISQTAGIIRDKIQVQEEIHTLTAARVFEQKI
                     MNGIPFGIILYIDFTSPGFFHVMYETWLGRIFMTACLGIYVGAVFLAKKILDIPL"
     gene            24032..25303
                     /locus_tag="ACZPCL_00745"
     CDS             24032..25303
                     /locus_tag="ACZPCL_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007035992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR1161057.1"
                     /translation="MKETKRKFYKKICKWKKQILCVGAGIFLGIAAWLAQGMDPVIEA
                     GNVIQRPDIDDGQVDQELYVKGLVDQEKEVVLKLPVSARQYTKEEAYQAYEEILKQLP
                     EWIKGDNLSLEEVRQDLELPAYWQGAGVHLSWQSSDPELVESDGTVHTWEFETGDEAI
                     DQWPVILKVRMTDGNWPEEYEIPIKVRPPLYTEEERTIQEFTSLLTSEEEVQKHQDQV
                     TLPSVYKDREISYSTAREPVFLQMCFLGAVAAVFISLKEKSDKKKAEEERKDQLLMDY
                     SEVLSRLIIFLGAGMSIRTAWDRIAEDYKMAVKEGRRGLRYVYEEMYVTSSQLKSGES
                     EARAFAEFGNRCGLQQYRKFSGLLEQNRKNGSKNLRETLRLEMADAFEQRKHQAKRMG
                     EKAGTKLLIPLFLLLAVVMAMITVPAWIAFG"
     gene            25387..25560
                     /locus_tag="ACZPCL_00750"
     CDS             25387..25560
                     /locus_tag="ACZPCL_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp1 family type IVb pilin"
                     /protein_id="HIR1161058.1"
                     /translation="MYRKIRDFALDETGVGVIELVLILVVLVSLVIVFKKQINTLLEN
                     IFKQINTKSKEVY"
     gene            25560..27488
                     /locus_tag="ACZPCL_00755"
     CDS             25560..27488
                     /locus_tag="ACZPCL_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5702 domain-containing protein"
                     /protein_id="HIR1161059.1"
                     /translation="MREKGEVTVFLAMILVMIMTLLLVMAESARTAGQRLYLRVASNS
                     AMESLMAQYHRSLWNEYRILGLETDSKGLLEEEFKGFLEPYMKAKNWYPLKTEDAVVK
                     DMAVLTEGKGSCMEQEILDYMKYGLAGILWESMTEGEAKEVLGDIKNAASVNSVSDLY
                     EEHSKEAVALEKALEKLNARLEQQKTHWEEGLDCLESLNGEGMIRKCEAVIKDLKAVP
                     GLVEAYEKKADQMERALKKSREKFVSEEDLKESSRGPLTKEIRSYETYISQDGERRNM
                     IRGLTEKSRENIRFMEQLIEEAEEVIQYIDDWEPSDEEDDLDEEELWEPVIRHMSRYP
                     VLTLGVSFGVKDKEKEGWLEKIKSMSGKGILKIVLPPDCQVSEKRLPLLQAPSALSKN
                     DTAHFQGISSLMDRLTVSEYGVRYFSHFEKIKDKDNFYELEYILYGKKTDRENLEASV
                     LKLVSVRQGLNMIHILSDGKKRQEAETLAAAITGGGSLLPLTAVVTFFVMGVWALAEA
                     MADARSLLDGGKVPLMKTGSDWKLSLENVLKTGAEGRLPDLEKTDPENEMGLDLKGYL
                     RLFLFVSYGTQPLFRMMDIMQINIRREQPDFLLEQCVCMVDMEAVFCGKPVFFSSGPW
                     KTAGKSRFAVTGSYLSVP"
     gene            27801..28547
                     /locus_tag="ACZPCL_00760"
     CDS             27801..28547
                     /locus_tag="ACZPCL_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161060.1"
                     /translation="MILIMPMILMDRQRQIQAAVESVGEEISQYAYVSYLYKSGKGET
                     LDVSGTNEREGSQLLEKVTAAYAVESVKRKISEQWVRDLSFYGTQITEDEMLQIIMKY
                     KMPLPFSLFGQDSVDVETVCSRRLWTGADGERGKKKAETGEQDERMVYIGKSSTRYHL
                     QSTCHYLFHNLTQIHSDDLVSIRNQDGKKYKPCSACKSSENAGPYYVLPYGTSYHSRE
                     DCRSLTAYVQEVPLRQVEYLGPCSYCAGGR"
     gene            28570..29061
                     /locus_tag="ACZPCL_00765"
     CDS             28570..29061
                     /locus_tag="ACZPCL_00765"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR1161061.1"
                     /translation="MNMKMGIEAALRICFLLFLLSASWQDLKKKTIKKSTFQIWGTVG
                     VILQAAHIFSQIFMGTEKMEMQVIVQEVTGLMFAALLGILLLSLSTATEEAMGKGDGC
                     FFLVAGIFLGFWKNMLLLSGGLFLCFPAAVYLMIKGRKARNTESIPFLPFVFPVGLGV
                     LFL"
     gene            29058..29495
                     /locus_tag="ACZPCL_00770"
     CDS             29058..29495
                     /locus_tag="ACZPCL_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161062.1"
                     /translation="MNKRGETVLKDERKKRKSIETEWKGSLTVEASCVMAVVLFSLAA
                     LIGKAGQIHDETAAAMVLHEGVEKCRHEKSIRSEDAEAFCKRNAGLMLRYTDLSVSIQ
                     EKGAKKMGKVKGGDWEKQIEMKEFRPEEFMRMVTGITGGTNEN"
     gene            29485..30927
                     /locus_tag="ACZPCL_00775"
     CDS             29485..30927
                     /locus_tag="ACZPCL_00775"
                     /inference="COORDINATES: protein motif:HMM:NF039422.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6382 domain-containing protein"
                     /protein_id="HIR1161063.1"
                     /translation="MRIEYIREMKRSYMTVTGEGAGESYETGMLAKNQISGLLKMKIK
                     YMDGVPVYCYDITSRQPVSRLFESRPAGENQVRSFFLQLYETLDRMEAYLLGDGGILL
                     DPELIYADPETFQMGFCVVPGRKEDFNSQLSLFLQYLMKNIDHRDRECVVLTYGLYQT
                     SMKDNYGMEDMMALLSKEEAKDLKTEKDLLMERKGEGKTETRPEIKTEKEDMAEESSL
                     YGLENDAQEERELFSEERTFANNGWSLWKKLLLLFLTAVMAAGGTWFLYGWSVLRKMW
                     VYGAAGFGIAILGTVLLHMMRGKGKGLLPGKYGGTGAKEPENSWQVFYEETEEAQNAE
                     KAEEIRQGSAPEKIPGYVPAREKTAERQQEKQLEEPQEECQTMLLTVKEEPDHMLVSA
                     GEENEDIAVGYVPFVIGKHTGLADYILNKSTVSRFHVRITEEDGQYFLTDLNSTNGTK
                     VNGRLLAANEKVVLSQGDEVWIAEEKYLWR"
     gene            30894..31097
                     /locus_tag="ACZPCL_00780"
     CDS             30894..31097
                     /locus_tag="ACZPCL_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161064.1"
                     /translation="MDRRGKVPLEIEKVIKRQEVTETDMERGRFFGDIAEMRLTREKN
                     YASIINKKSAYRHRRGGIKNGRR"
     gene            31084..31503
                     /locus_tag="ACZPCL_00785"
     CDS             31084..31503
                     /locus_tag="ACZPCL_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR1161065.1"
                     /translation="MAEDNCIFCKIARGVIPSTTVYEDEDFRVILDLGPASKGHSLIL
                     PKKHFANVCELDAETAAKVLPLGAKIGAAMKKGLGCAGFNLVQNNGEAAGQTVMHFHM
                     HVIPRYEGGPANIANWVPGTASAEELTEAAEKIKGCL"
     gene            31510..32058
                     /locus_tag="ACZPCL_00790"
     CDS             31510..32058
                     /locus_tag="ACZPCL_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1161066.1"
                     /translation="MIKRMDEEAKASFEEMYLTYQDTLRRLAYAYDIPVDDIDDIIQD
                     TFVSYARYDYSLKQPEEGKKILLGRILRSRCMDFHRQKKKRYCEDLNGETFAAEEYRM
                     QLHQPGLVDVFSGKERCKAILDEVDRMPENWQKVAELKLIEGRPTSEVCDQLSISEKA
                     CYSRVSRIRKYIKSLLKREDWP"
     gene            complement(32128..33198)
                     /locus_tag="ACZPCL_00795"
     CDS             complement(32128..33198)
                     /locus_tag="ACZPCL_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR1161067.1"
                     /translation="MKKIILTGGGTAGHVTPNLALLPSLKKAGYEIRYIGSYQGIERK
                     LIEGAGIPYDGISSGKLRRYFDLKNFSDPFRVAKGYFEALRLMKRYKPDVVFSKGGFV
                     AVPVVLAAKHYKVPVIIHESDMTPGLANKICIPSAAKVCCNFPETLKYLPKDKAVLTG
                     SPIRAELLEGDRKAGLSYAHLTEDKPVLLIIGGSLGSVAVNTAVRKILPQLLNTFQVI
                     HICGKGNLDESLIGTEGYVQYEYVDAPLKHLFAAADVVISRAGANSICELLALRKPNL
                     LIPLSAAASRGDQILNADSFARQGFSKVLKEEELSDETLSTAVFDLYKNRETYINAME
                     KSSLSNAVETITSLIESCVSHS"
     gene            33336..34112
                     /locus_tag="ACZPCL_00800"
     CDS             33336..34112
                     /locus_tag="ACZPCL_00800"
                     /inference="COORDINATES: protein motif:HMM:NF025643.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HIR1161068.1"
                     /translation="MNDWMGNQNKKREKLTDSDILKQFEQAEKDTRFLPINPPPKDEF
                     DQIWSRIQAEQALFSEISNTTNTKSSRKTRRRPVRKALVVGLAAAIMVIGGCFVAMGT
                     KSYFYRDGRSVNQGVVYNNDSGALTVRSEEEAYEKIAEELGVKTLKLGYMPEGMRFEN
                     VIIDNGYGRLQYNYKNSKVLFVQTKSDDKTSMVHSSDAREMNEIWNQGLHMNISIYGE
                     IIAKNRKRYEASFSCNGVYYYLFSEMQQDQFEEMLKKVHF"
     gene            34128..34649
                     /locus_tag="ACZPCL_00805"
     CDS             34128..34649
                     /locus_tag="ACZPCL_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161069.1"
                     /translation="MKKVIRLLVSVLAIVNIFVGTAFAGIPSPEIYTSVNQESKLCDK
                     NISYSTGTAKSYARRGRFFVSADVAIKDEGNGNIGASAYAYLSKPVTELYVSMCLDRY
                     NANIDAWEQVDYVDFEYTLEEYPDGIEDPIIHVTFENQKRGYYYRIRGAFSAGDDDWY
                     EGFGPTTDGIWIE"
     gene            35061..35489
                     /locus_tag="ACZPCL_00810"
     CDS             35061..35489
                     /locus_tag="ACZPCL_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR1161070.1"
                     /translation="MRDYLNRLRQQFQRFMIGRYGMDQFGRFLSGVLLALIIFNLLIP
                     AALPSRILWALEWIVLIILYARMFSKNINKRYQENEAFLRRQFYVSEWWKKWKFRYQE
                     SRKYHIYRCPNCKQKVRVPRGRGRISIHCPKCGTDFIKKS"
     gene            35738..36676
                     /locus_tag="ACZPCL_00815"
     CDS             35738..36676
                     /locus_tag="ACZPCL_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1161071.1"
                     /translation="MKKNGMKVMAMIGSAVMAAGMLAGCGNSGAAATTAAPATTAEQT
                     TEAKAEETTKEAATEAKSADADLSGSISMVGSTSMEKFANALSEAFMEKYPKVTVTAE
                     FVGSGAGIEAVSNGTADIGNSSRNLKDEEKAKGVAENIVAIDGIAVVVDPANTVEDLT
                     KDQLTSIYDGTVTNWKDVGGNDAPIVVVGREAGSGTRGAFEELLKLEDACKYSNELDS
                     TGAVMAKVASTPGSIGYVSLDVLDDTVKALKLDGAEPTEENIKAGKYFLSRPFVMATK
                     GEISEQSDLVKALFDFIYSDEGSELVKSVGLITADK"
     gene            36756..37688
                     /gene="pstC"
                     /locus_tag="ACZPCL_00820"
     CDS             36756..37688
                     /gene="pstC"
                     /locus_tag="ACZPCL_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR1161072.1"
                     /translation="MTQETFSVRSHHGSKSTVERAAEAIFTACGFFAVLAVASITLYM
                     IFSGTPALFKVGILDILFGTLWQPAAATPSFGILYVILTSIVGTFLAILIGVPVGVMT
                     AVFLAEVAPKKLANVVRPAVELLAGIPSVIYGLLGILILNPLMYKMELAVFKGSSTHQ
                     YTGGANLISAVLVLALMILPTVINISESALRAVPGHLKSASLALGATKIQTIFQVILP
                     AAKSGIITAVVLGTGRAIGEAMAITLVSGSSVNFPLPFNSVRFLTTAIVAEMGYASGL
                     HREVLFTIGLVLFAFIMIINIGLNSILKKGEKTK"
     gene            37685..38587
                     /gene="pstA"
                     /locus_tag="ACZPCL_00825"
     CDS             37685..38587
                     /gene="pstA"
                     /locus_tag="ACZPCL_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR1161073.1"
                     /translation="MTGEAAIRINENSVIRESIYGKRIRSTDVILTGIIYGCASLSML
                     LLIGILGYTFVRGVPHVTWSFLSTVSSATKGTFGILGNIINTLYIVVITLLIATPIGV
                     GSAVYLNEYAKPGKIVRLIVFTTETLSGIPSIIFGLFGMVFFGNTLGLGYSILTGSLT
                     LTLMILPLITRTTQEALKTVPDSYRHGALGIGATKWYMIRTILLPSAMPGILTGVILA
                     IGRIVGESAALLFTAGSGYYLPKNLFGKVLESGGTLTIQLYLFMQKAKYNEAFGVAVV
                     LLVIVLAINALAKYLSHRFNVETR"
     gene            38619..39380
                     /gene="pstB"
                     /locus_tag="ACZPCL_00830"
     CDS             38619..39380
                     /gene="pstB"
                     /locus_tag="ACZPCL_00830"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR1161074.1"
                     /translation="MENKVKISTQNLNLYYGENHALKNINLDLYENKITAFIGPSGCG
                     KSTYLKTLNRMNDLVPNVKIEGTVLLDGEDIYDPQVDTTLLRKKVGMVFQQPNPFPMS
                     IYDNVAYGPRIHGIKNKAQLDEIVERSLKGAALWDEVSDRLKKSALGLSGGQQQRLCI
                     ARALAVEPEVLLMDEPTSALDPISTLKIEDLMDDLKSKYTVAIVTHNMQQATRIADYT
                     AFFLVGEVVEYATTDELFTMPKDKRTEDYITGRFG"
     gene            39460..40116
                     /gene="phoU"
                     /locus_tag="ACZPCL_00835"
     CDS             39460..40116
                     /gene="phoU"
                     /locus_tag="ACZPCL_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR1161075.1"
                     /translation="MRITYEHELEELNKCLRDMAMMVEKAIEQTFVAFEDQNYTMAED
                     VIKGDRNVNDMERAIESRCLSLILRQQPVARDLRQISTALKVVTDLERIGDHASDIAE
                     LILRIKSDHAYHIVQHLPAMAASAQKMVHDAVEAFIKQDVEEAEEIIRRDDEVDALFN
                     QVKSDVVELLKSAPSHADQGIDLLMIAKYLERIGDHAVNVCEWTQFCKTGALKNVRIM
                     "
BASE COUNT        11847 a         7662 c        10360 g        10498 t
ORIGIN      
        1 tcttcagtgg cccatttttt tatcggtcct gatggtcctg ctgacagctg ctgtgggggc
       61 agtttcatta agagcaggca ttatagtttc attttttact tttatctata ttggaatagc
      121 gttatggatc tacttttcca gaagaaaggg cattcttgcg ggactgatcg cattttcaca
      181 ggtctacgac aagagcaaaa gtaaatttct ggaagaaatg ctggtgcctt actgtgtggc
      241 ggatatagca ggtcgtattt tgtggatgaa ccaggaaatg gaagccattc ttggggatga
      301 taaagcaacc atcaaaaaca ttacccagat gtttccggat gtcacaaagg aaatgcttgc
      361 caccggtgga gaaactgtaa gtatccacag ctcttttaaa gataagaagt accgtgtgga
      421 catgaaagag atccatacag aagaggctca ggcagcggca gctgactgtg gactggatga
      481 gggaaattcc ctggtaacag ccatttatct gatcgatgag actcagatgc tgttatataa
      541 gcagcagatc aatgaccaga aactggtagc cggtcttatt tatctggaca attatgatga
      601 ggcgctggaa agcgttgagg aagtacgccg ttctctttta acagctttga ttgatcgaaa
      661 gatcagcaag tatatttctt ccatgaacgg tattgtaaaa agtattgaga aggataaata
      721 tttctttgtg atcaagcagc agtatgttgc aaagatgcag gatgagcgtt tttccattct
      781 ggaagatgta aaaacagtca atatcggtaa tgacatggca gtcactttaa gtatcggtat
      841 tggtatgaac ggtgaaagtt atgcacagaa ttatgattat gcaagaactt ccatcgatat
      901 ggcacttggc cgcggcggtg accaggcagt tgtaaaggac tccgataaga tcctttatta
      961 cggcggaaaa gcccagcaga tggaaaagac cacccgtgta aaggcccgtg tgaaggcaca
     1021 tgctttaaaa gagctgatgg ataataaaga ccgtctgttg atcatgggac acaggatggc
     1081 agatattgat gctttcggtg cggcagttgg tatttaccgt attgccatgt ccatgaataa
     1141 aaaggcaaat atcgtactca atgatgtaac ttcttctgta cgtcctatga tggaacgttt
     1201 ccagaacaat ccggagtacc cggatgatat gttcttaaac ggcgccaagg ctgcggagct
     1261 ggtggatgca ggtactatgc tggtggttgt ggacgtaaac cgcccaagta ttaccgatga
     1321 accgagcctg ctgcgtctga tccggaccat tgtggttctg gaccatcacc gtaccagcag
     1381 cgaagtgatc gacaatgcgg ttctttctta tgtagagcca tatgcttctt ctgcctgtga
     1441 aatggttgcg gaagtattgc agtatattgc agacggcatt aaagtaaggc cctgtgaggc
     1501 agatgctatg tatgcaggta tcgtgatcga tacccagaat tttaccaacc agacaggcgt
     1561 gcgtaccttt gaggcggcag cattccttag aagaagcggc gcggatgtaa caagagtgcg
     1621 caagctgttc cgtgaaaata tggtagatta tcaggcgaag gcagaggcag taagggctgc
     1681 agaggtttat atggacgcat ttgctatcag cgtctgcccg gcagaagggt tagacagccc
     1741 gacgatcatc ggtgcccagg cggctaatga gcttcttgag atcaagggca ttaaggcttc
     1801 tgtggtactt actgattaca ataataccat ttatttcagt gcccggtcta ttgatgaggt
     1861 caatgttcag gttatgatgg aaaaactggg tggcggcggt cacaggacta ttgcaggtgc
     1921 ccagatgcag ggagttacca ttgaagaagc caaggagcgc ctgaaagcgg ttatccgtca
     1981 gatgatggat gaaggtgatg tgtcttaaca gacagtatcg aagatattca gagatataag
     2041 atatagagat attcagatga tatagaaagg aagttttgta aaatggaaat cgtattatta
     2101 gaagatgtaa aggcattagg taaaaaagga caggttgtaa aggtaaatga cggttacgca
     2161 cgcaacttca tccttccaaa gaaattaggt gtagaagcaa cctccaagaa cttaaatgac
     2221 ttaaagcttc agaaagcaaa tgatgcaaag atcgcagcgg agcagctggc agcagctaag
     2281 gaactggctg agaagttaaa caatgcttct gtgactgttt ctatcaaggc tggcgaaggc
     2341 ggaagagctt tcggtgctgt ttcttctaag gagattgaga aggcagctat ggatcagtta
     2401 ggtctggaga tcgacaagaa gaagatcgta ttaaacacac cgatcaaggc aatcggaagc
     2461 tttgaggtat ctgtaaagct tcacaaagaa gtaactgcaa aactggcagt taaggtagta
     2521 gaagcataaa gataacacag tccgggaaca ctacccggaa atgtaaggag ataagtttat
     2581 ggaagagacc ctgatcaagc gggtgcttcc tcacagtata gaggcggaac agtctgttat
     2641 aggttctatg ttaatggaca gggaagcggt aatcgctgca tcggagatca tcacgggcag
     2701 cgatttttat cagcagcagt acgggatcat gtttgatgcc atggtggaac tgttcaatga
     2761 aggaaagccg gtagacctgg tcacacttca ggaccgttta aaggagaagg atgttccgcc
     2821 ggaagtcagc agcctggatt ttgtccggga tatcattacc attgtgccta catcagccaa
     2881 tgttaaatcc tatgctacga ttgtcagtga gaaagcagtt ctgcgccggc ttataaagac
     2941 aacagaagag atcgcaaata cctgttatgc aggaaaagag ccactggaaa atattctggc
     3001 ggatacggaa aaatctattt ttgacctgct ccagaacaag ggcggacagg aatttgtacc
     3061 catcaagcag gttgccatca atgttctgga aaagatcgag gatgcatata aaaaccaggg
     3121 aacggttacc ggtatccctt ccggatttat tgatctggat tataaactgt ccggtttcca
     3181 gccttccgat tttatcctga tcgcagcccg tccttctatg ggtaaaacgg cttttgtctt
     3241 aaatgtagtt gactatgttt ctgtgcgtaa gaacctgccc tgtatggtat tcagtctgga
     3301 aatgtcaaag gaacagctgg taaaccgtat gctttccatg gagtccaatg tagactccca
     3361 gaagctgcgt acaggtacgc tgacagactc tgactgggat gcggttgtgg aaggtatcgg
     3421 cattatcggt aattccaagc tgatcattga tgatacaccg ggtatttcca tttctgagct
     3481 gcgttccaag tgcaggaaaa tgaagctgga atatggctta agtattgtta tcattgacta
     3541 cttacagttg atgagcggaa gccgcaaagg gggaaatgac aaccgtcagc aggaaatttc
     3601 cgatatttcc cgttctttaa aggcactggc aagagaactt catgccccgg tcattgccct
     3661 gtcccagctg tcccgtgcct gtgagacacg tacagaccac agacctatgc tttccgacct
     3721 tcgagagtcc ggagccatcg agcaggatgc ggacgttgtt atgttcttat atcgtgatga
     3781 ttattataat aaagatacgg aacatccaaa tgaggccgaa gttattatcg ccaaacagcg
     3841 aaacggccct atcggtactg taaacctgat ctggaaaccg gagtacacca agtttgtaaa
     3901 tgcagtgcgt cctcagggtg gaaatcagga aggataatgt atatgatctg gcacttgtat
     3961 cagttgatgc aggtgccatt tatattttta gtcataatag tcattctcaa cgcatacatt
     4021 gataataaac agcggcaaaa gggctgcagt taatcagaat gatacgtgtt gtgaaggaga
     4081 gaatggctat ggatgaagta cattatctta tatcggatgc atccagaaag gtggatgtgg
     4141 aagcccatgt attaaggtac tgggaggagg agttagaact tgcgatccca cggaatgaaa
     4201 tgggacacag gtattatacg gattttcata tccggctgtt taagcaggtt aaaaatctga
     4261 aggaaaaagg gtatcagtta aaggcgatca aacatgcttt agaacagata aaaaaggacg
     4321 gaaagctgga aggcgaactg acagatgaga ttttagagca ggatatggat gctgctttaa
     4381 aagaacttaa ggaagaagag aaagaggaaa atacagctat ggtaagcaca tctgcagatg
     4441 gaagtccgct gcctgtaagc gcagaagaga aaatggccca gtttcagcag atgatgaacc
     4501 tgatcattgg acgggctctt gaggtaaaca atgaaaaact gagtcaggat atcagctgcc
     4561 tggtaaaaca gcggctggca ggtgaattgg aacttcttct tcaggagtcc ggccagaagg
     4621 aagaggaacg ttttcgtctc ctggatgaaa ccatccgttc cttccagaaa gcaggacagg
     4681 aggaagctgc tgcaacaaaa gtgccatttt ttaaaagaaa acgctttgga cggggggcta
     4741 agaagctttt tcacagggaa aagagaagtt aaagaaaata agaaaaataa taaaaacaaa
     4801 gaaaaagaag gacaagccag ggagcgctgt tatggaagct gtttctggtt tgtccttttt
     4861 tgcgattttc ttaagactgt gaaaaatatc gaaaaatagt aggatatgtg ctataatctg
     4921 ataagcgcaa gcaggcagta gagcgcgcaa gcgcgtggtc ctgagtgaaa ctagtggaag
     4981 cgaaatcttc agggattgga tttacagaag caacaaacaa caagcgagga tacatttcat
     5041 gaatgaaaaa tatacatatt atctgcgata tgcctgcata tccagtgcgc tggcggcagc
     5101 gttggcgtta ggaggctgtt cttcagggga aagaggagtt gcaactcaga atgaggctgt
     5161 agctgcaaat gcccaggacg gacaggcagc agtttcagat ggaacagatg agaccatagc
     5221 acctttagga atggatgggc agatccttcc tggactggat gatgtagaat tgccggaaga
     5281 tgcacaggca gcgcctcagt atttaagaga aggtgtacgc catgagatcg taaagaagtt
     5341 acagcagcga ttaatggact tgggctttat ggataacgat gaacctactg actattttgg
     5401 tcaggtcact cttacagcag tgaaacattt ccagagacag aatgaactgc ctcaggatgg
     5461 tattgtaggt gataccacct ggaatgaact gatggcagat gatgccaaac attacgcagt
     5521 atcaaaggga acccaggggg atgatatcca gaagatccag cagcgattat acgaactggg
     5581 ctatctggct tctgcagacc aggtaacagg caactttgac gatgccacag aaaccgctgt
     5641 tttaaaactc cagggcgtca atggccttgc agaagacggc aaggtaggcc agcagaccta
     5701 taacctgatg tacagcgatg acattaaggc aaatatgctt gcatatggcg aaaagagcga
     5761 tgtggtatta gcatgccagc agcgtttaaa ggatctgggc tatctgacta ctacaccgga
     5821 cggtacctac ggtcaggaca ctgtgatcgc agttaagcag ttccaggcaa gaaacgacca
     5881 ggtagtagat ggttacttag gaccatccac acgtattgta ttaaacagtt ctgacgcaaa
     5941 gccaaacggc ctgatgatcg gcgaacaggg tgattctgtt accagagtac agcagctgtt
     6001 aagcaagtac ggctatctgc cgtcttccaa tgtaaccggc tactatggtg aagcaacaga
     6061 agacgcggta aagaacttcc agtcacgaaa cggcctgtct gcagacggta aagtaggcgt
     6121 acagaccatg gcaaagctga ccagtgataa tgttaagaag ccggcgccta ctacagcagc
     6181 gtcaactaag agtaataaat caaatagcag cagcaataag aataatagct caagtaaggg
     6241 caacagcggt ggaaatacca gctcaccggc tccgagacca gcttccggtg caggtgtttc
     6301 agctctgata tccgtagcat cttccaaact gggaagtcca tatgtatggg gtgcaaaggg
     6361 accaagctcc tttgactgtt ccggttttgt atattggtgc ttaaagcagg taggcgtgag
     6421 ccagagttat atgacttctt ccggctggag aagcgcagga cgttatacaa aggtaagcaa
     6481 tttcaatgat atccaggcag gagatatcgt agtagtaaga ggccatgtag gtatcgctgc
     6541 aggcggcgga acagtcatcg atgcttcttc cagtaacggt aaagttgtgc acagaggttt
     6601 aagttcctgg tggagaagca actttgtctg cgcttggcgt atcttttaaa ttaagttgtc
     6661 ccgcgcctgt cggcgcggac gcaggtggta caaattgcag caaatttgcg cccggccggg
     6721 cgcagaagga tatatcaata aaaggaggga catttatgca gacgattctg gtatgtgatg
     6781 acgataaaca gattgtggaa gccattgaca tttatcttac aggagaaggc tttaaagtta
     6841 taaaaacata tgacggttat gaggctctgg aatatctgga gacccagtcc gcagatcttt
     6901 tgatcttaga tgtaatgatg ccgggactgg atggcatccg cactacttta aaggtacgtg
     6961 agaccagcag tatcccgatc atcattcttt ccgcaaagtc agaagatgca gataagatct
     7021 taggtcttaa tattggtgca gatgattata ttacaaagcc atttaacccg ctggaactgg
     7081 ttgcaagagt taaatcccag ctgcgccgtt atacacagct tggaaacatg aaccagcagg
     7141 cggaaggaca ggtctacaag tgtggcggtc ttcagatcaa tgatgataac aaagaagtta
     7201 cagttgatgg agatccgatt aagctgactc ctattgaata caatatcctc ctgcttctgg
     7261 taaagaacgc aggaaaggta ttttccattg atgagatcta cgaaaagatc tggaatgagg
     7321 aagccattgg cgcggataac acagtagctg tccatatccg ccacatccgt gaaaagatcg
     7381 agatcaaccc aagagagcca aaatatctga aggtagtatg gggcgtaggc tataagatcg
     7441 agaaacagta ggtggcctat ggaaaaaaga gcgtttggtc tgagaaaatg gaaggtgctt
     7501 ctgtcagtgt ttcatgtgat atttcttgtt atcacactgg cagggatcag cgccatgtac
     7561 ttaaacggcg ggtatggaaa agggatccgc tggatctatg atgagactta tgaagactca
     7621 gatgccttct gttcccagct tggcatagat atcagcaata tttttaccta tatcggatat
     7681 aaggatatgt ttgagaccaa tggtgtcctg gatatgcaca aacccattgt gcgtattata
     7741 gacggtcccg gtgtagatga ggatatgaca ctggatcaga tcgtccgcta tgccaagatc
     7801 cggggatatt atctgggaga agattttaca gtacagggat ctcctatggc catggatgat
     7861 gatgacatgg agataacggt agagtataaa tccgcaaaaa cggacctggc agatgttgat
     7921 ggcctggaga ccaggatgac caaagaggaa atggcactgg acatcctgga ccggttggga
     7981 aaatattata ccatttatta taatttcatc agcaataaca caaatgtccg tttccgtatc
     8041 ctctataaaa gcaatgatgg aagagaagtg gtttatacca atgttccttc tatgtcttta
     8101 gatgagctgc ggggatttgg aaaatatctg ttcattcccg gaaataccat tcgtatggaa
     8161 agcaatcttg caacgatccc ggacaatgcg gcttctcttc tggaaacatg gaatcccttt
     8221 gacaatgacc agaattatat ggtggtctct gtggatacta cctatcctta ttctgataat
     8281 tatgcagagg aagccgggga atataaaagt gccagatcca cgtttatcat gggaatgggc
     8341 ggtgtgatcg taggaattgc aggatttttt gctacactgg taacattggt ccttttatcc
     8401 ggccatgaag atggagcaga tggcaagatc cgtcttttcc ccatcgacag gatctataca
     8461 gaaacctgtg tccttttgtg ggcggtggtg acagctgtga tcttgtactt ctgtaaaaga
     8521 ctggggatga tggtgatcgg cttatttgcc tccgaagaac acctggcata ctggagtaaa
     8581 ctgctgaaat atgtgatcat ctacgggtgc agtattctat gcggtttcag tcttttaaga
     8641 agatacaagg cagataccct gtggaaaaag agtattgcaa aaaaggtagt aaaggcttct
     8701 aaggattata ttgcaaaagc cagttttgta gccgggatcg gactttgcta tcttctgttt
     8761 ttaggagcca atggcgtaat gctgtggggc attttattcc tgtttgccta tatagatgag
     8821 aaactgactt tccgtattct cttttatgta tgtgctgttt tatttgttgg tctggatgga
     8881 tgggtattcc ataagctgtt caggaaagcg gagcagaagg atcttttaaa taatgcgatc
     8941 acctgtattg caaaaggcga taccggtttc cgcctggata cgaaaaagct gacaggaaaa
     9001 gagcggacag tgggagacca cattaataac attggaagcg gcttggacac ggctcttcag
     9061 gagaaggtaa agagcgaacg cctgaaagcg gatctgatca ccaatgtgtc ccatgatatt
     9121 aaaacgcctc ttacgtccat tatcaactat gtggatctgt taaaacgcca gaagatacag
     9181 gatcctaaga tcgcttccta tctggaagtt ttggaccaga aatcccagcg tttaaaaaca
     9241 ctgacggaag atctggtgga agcttccaag gcaagttccg gaaacctgaa gctggagatg
     9301 gcagatattg acctggtgga gctggtacag cagaccaatg gtgaatttga agagcgtttt
     9361 gaacagcgcc atttaaagat catctctgat ttcccagatg gtatgatcat tattagagca
     9421 gatgggcgca gattatggcg tgttctggaa aatctttata ccaatgcttt taaatatgcc
     9481 caggaaggca gccgggtata tgtggatgtg gcttctgtag atggaaaagc cattttcact
     9541 atgaaaaata tttcggaaaa gccgttaaat atcagcccgg atgaactgac agaacgtttt
     9601 gtcagaggag atgtggcgcg gaccacagaa ggcagcggat taggactttc cattgcccgg
     9661 agcctgaccc agctgcagaa aggtgagttt gtgatcacca ttgacggcga tctgttcaag
     9721 gctcaggtta ttttcccgca agtgcgccag gagaccaggg cggaaatgcg tctggaacgg
     9781 gctgcggaag agaagcaggc agaagaacag tccggggaga aaatgtccgg ggaaatgcct
     9841 gtgggagaga acctgctgga aagtgcccca tataactggg atgtaatggt agaaaatgac
     9901 aagaatttaa cagcaaaaga ggaaatcctg atccagaatg gaaaacggga gcataaaccg
     9961 gaaaaataaa tagaaaatgt aaggaagatc ctaaaaagag acaggcccac aaggaacctg
    10021 tcttttttag attcggattt aatgaccgga aactttatag cagaggtgtg acttaggagt
    10081 atcagagagc aggtattctt ctgtagtcat gagtactgtg tggagcggag caaagtaaga
    10141 gataatgttt tcggtttctt tgcgggaaac ttcactgagt ccggctacgg catcggtcag
    10201 gtaaatgact ttacagccgg cgtcaatggc agaaagcaca gtagagagta cgcagcattc
    10261 tgctactacg ccggagatga caagatgccg ggcttttttt gcggctttta caacctcggg
    10321 gatggcaaag gaggaataga cacttttggt gaataccggg tatgttttta cccatggaag
    10381 gaattccggc atcatttcat tgagccatgg atcagagttt acagccgcat taactttatt
    10441 atattctttc cagacaccgt ccgggtgttc tgtagccaga tattgggtaa agatgacctg
    10501 ctttgttttt tgactgttta atagacggag gatggaggca gatgcatgtt caatgccttt
    10561 acaggcccat tcctgtcctt ttgtataaac attctgcatg tcaatgacta acagcaggtc
    10621 tgatgaattg ttttgttcca ttctgaaaat cctccgggag aacagtatta atgtattttc
    10681 agtatagttt tacacttaaa attttacaag caaaacaata tgttaattta ttaacaataa
    10741 gactctgaat tggctcattt cattgacttt gtgtaaggat aatgttatct ttatcgtaaa
    10801 ttgttgttaa attcataaat gggtgtacat tttcctgatt tagaaggaac aacatgaaaa
    10861 aatctggtgg cagatataca aggtacaaag tgtcatacag ttgggaagga gattgtgaaa
    10921 tgggagaaaa taaaatgccg catatccagc ttccggagaa tctgggaatc cggtatgcag
    10981 tgctgccggg agatccggcc cgggcagagc gggcagcagg ttatttaaaa gatgcaaagc
    11041 tcttaacctt caaccgggag tataaaagct acagcggttt ctggaatggt gtgccggttc
    11101 tggtcatgtc caccggaatg ggtggtccgt ctatggcaat tgctgtggaa gaactgcaaa
    11161 agatcggtgt agaagcggct gtgcggatcg gaagctgcgg tgcgctgcag ccggaggtca
    11221 gagtcggaga tctggttatg gtagaagccg ctgtacgtga tgaaggaact tcacttggat
    11281 acgcgccact tagttatccg gctgttgcag accacaggct gcttaccgcc tgtgaggaga
    11341 gcgcaaagga ggccggctgc cggttccata ccggcattgc cagaagtcat gactgcttgt
    11401 accgggatga caatccggtt atttatgagg aatgggcaaa gaaaggcgtg ctggcgtctg
    11461 acatggaaac tgcggcactt tttgtggttg gcaggatacg tggtatgagg acagcatcta
    11521 ttttaaatgt agtggcagcc tgcaagggca atgtagctga aaacatcgga cgatacgtgg
    11581 atggtgagac attgactgct cagggcgagg aatatgagat cttgacagcg tttgcagcgt
    11641 taacaagaca gtaatgcagc aaaaaataca gcttctgaaa ctggagcgat caaatgggaa
    11701 aggatgtatg ttatgaagaa tttattcaaa actaaatttg atgtggcagt aatcgtattg
    11761 attccggcct gcatcggtat taactatctt ggaaagctgt ttgccagcgt gttaaagctt
    11821 ccgttgtggc tggattccat tggaacctgt atcggtggat gcctgggtgg tcccattatc
    11881 ggagcaatct gcggagcagc taataacctg atctacggat tttccgcagg tgataatatt
    11941 acgttgattt atgcactgac cagtcttggt attggcgcgg cggcaggaat catggcacgt
    12001 ctgggcttta tgaagtcctt cccgaaggct atcatcaccg cctgtgtggc aggttttgtg
    12061 gctgtagcta tttcaactcc gttgaatgtt ctgttctggg gcggcactac tggtaacgtg
    12121 tggggtgatg ctgtatttgc agcaacccag gcagctaata tgccggtatt cttgggttct
    12181 ttattagatg aggtcgtagt ggatgtaccg gacaagctga tcacgctgat catggtattc
    12241 tttatcttaa agggtcttcc aaataaactg actactctgt atgaggtaaa ttctgagatt
    12301 gagactctgg actgatcaag aaaaggaaat ataacagaac atgaaaagta ttagcctttt
    12361 tgtagataac gggacttacc tgaccaaggt gcatcccttc accaaattga tgtacatagc
    12421 cgccgccatc agtattccgc tgatttccgg gcggctgtgg atgtttggcg tgtttattgc
    12481 ggtcagcctt cttgtgctgg ccagtggcaa aattttaaaa aagacccttc cgctgattgc
    12541 tttttccttt acgatcttgc tgaccatttt tctgatccat ggattgttca accagaaaaa
    12601 cgtcaatgtg ctgtttcagg tcgtcggatt gaaattttac cgggaaggca tattttatgc
    12661 tcttcatatt ggtctgaata tcctaaatat gctgttgtct tttgcagtat ttgtgctttc
    12721 cacaaagccc tctgaatttg ttgatgagct ggaaaaacgg ggattttccc cacggttcgg
    12781 ctacattgtg acctcagtat tccagatcat cccgcagatg atgggaacga tgaatactat
    12841 catggatgcc cagcgcagcc gcggactgga gacagaagga aatttgagaa cccgtgggaa
    12901 ggcatttctg cccctgatct cccctgtggt catgagttcc ctgatcaata ccagagagcg
    12961 tgccattgcc ctggaggtgc gtggttttgg cgcaggaaag aaaaaaacct ggctgacaga
    13021 gcggacacgc cataaaggag accgtgagat cactatcttt ttaggtgtct gcattttggc
    13081 ggctgtggta tggaggattg taggatgtct gtgataaccg ttgaaaattt gcgataccgt
    13141 tatccccaca cgaaggaact ggcgttggat ggactggact tttcggtgga aaagggagag
    13201 tttattggca tcatcgggga aaacggagct ggaaagagta cgttaagtca ggccattatg
    13261 gggctggtgc cgcagtttta taaaggagct tacggcggaa tggtcacggt agatggcatt
    13321 gaggcaggca agactccggt ggcacagctg tgcggccatg tgggactggt attccagaac
    13381 ccctttaacc agctttccgg cgcaaaggat aacgtatacg aggaagtggc atttggcatg
    13441 cagaatctgg gtgtgccggc agaagaaatg aaaaaacggg tagaggaagc cttaaagctt
    13501 ctggatatct ggcagtaccg ggatcgcaat ccatttgatc tgtcaggcgg ccagatgcag
    13561 cgcgtggcta ttgccagtgt gctggttatg cggccagatg tgatgatcct ggatgagcct
    13621 acttctcagc tggatccaga gggaagtgac gaggtattta gggcagtgga aaccctgacc
    13681 ggctctggta tcaccattct gatgatcgag cagaagattg agaagctggc ggcctactgt
    13741 gaccgcattc tgcttctgca caaaggaaag cagattgcat tcgatacgcc gcagaaggta
    13801 ttttctatgc cggatttaaa cgattatggg atccaggcgc cggcatttac ccgcatttgt
    13861 aaagcagaac aggtgactct ggcagatgga acgtatccgg tgacagtgaa agaggcggct
    13921 gaagtgctga gagagaagcg gctaggtgta tatgagcatg cgaatgcgga agcaggtgtg
    13981 caaaaagcag tgggagcgag tgcggcaggc accggacagg tacaggcaaa gaacgctgaa
    14041 acaggcgcaa ggtccgggca gtctgggact tgcaggaagg ctgaaaacgt tgcttctgtg
    14101 gatgagcagt tccggattga aaaactggat ttctcttatc tggcagatat tccggtttta
    14161 gaggatctga acatgaagct ggatcagcgt cctaccgcca tcatcggcca gaatggagca
    14221 ggaaagacca cgctggtcaa acttttaaag ggtttgttaa aaccggtttc aggttccatt
    14281 tatttccacg gtgaggacat ttcaggcaag acagtggcta tgctggcagg aagtgtaggt
    14341 tatgtattcc agaatccgga tgaccagatc ttcaagtaca atgttatgga tgaggtcctg
    14401 ttcggcccgt taaatatcgg catggatccg gaacaggcga agaaagaggc agcctgggcg
    14461 ttggaactga caggactgtc tggcaaggag aaagaaaatc cctacgatct ggaactttat
    14521 gagcggaaga tgacagccat tgcctcagta cttgctatgg acaccgatgt actgatcctg
    14581 gatgagccga caattgccca ggactggaaa gggcggcaga tcattggcag cattatccgc
    14641 agcctttctg ggcggggcaa gctggtcata gctattcttc acgatatgga ctttgtggca
    14701 gagaattttg aacgggttat catcatggcc catggtcagg tgctggcaga tggaactgca
    14761 aaagaagtat ttgcccagga agaagtgctg aagaaggcac gcctgcagaa gccatatgtg
    14821 atgcagctta gcgaggcact tggatatgag aagagctatc tgacggtgga ggaacttctg
    14881 aaagatcgga tgtctttgtg tgctgcaatt aagctttgaa aaaattccat tacaaaaact
    14941 cctgcctaaa gcaggggttt ttgtatttgg acatagaaaa acagcgaata aacaaaatgt
    15001 tacccactgc aaaaggaatt cttaattaaa aagacaggct cgcagggggc ctgtcttttt
    15061 ctgaccgtaa gtttgcagtc cgctgatcgg cggtcctgcc taaatgtgta aatagatttt
    15121 actgatactc aaatacattg atccagctct ctaacagatc tttctcttcc ggacgcttat
    15181 aagccagctg tgcggctgct acgattggaa gccagccctg tacatagcgt ttttctgtac
    15241 ctgtcttttc acagaacaga tccatgtaca gttctgcttt ctttttgtcc tttaagctga
    15301 gcagaagata ggttctggcc acgtcggcac ttgcattacc ctgggaagca tgtacccagt
    15361 caatgacata gagctttccg tcatcaccaa caatgatgtt ggatggattg aaatcgccgt
    15421 ggcagagttt ggtgtgtttt ggcattccgt ccagtctggt caaaagctca tagcgtgtgc
    15481 tttcatccag ctgttccacg gattttaagg aacggatggt cttttctttc agtttgttga
    15541 gcagcgggca agcctggctg tggatcaaca gctgcagatc taccatctgg gacatgtact
    15601 cgtccagctt gtccggattt tcatccatca gctgttctaa ggtcttacct ggtacatatt
    15661 ccttgataat agaccatttt ccatcagtta ccgttacccc caaaatcttt ggaacattga
    15721 gcgcttcaat ggtctccact cttgcattac ataatgcctc gtttaaaacc tcagctttcg
    15781 gaaatccttc gcagaattcc ttaatggcag tatcttcgtc acggtagacg gtcttttttg
    15841 cactactgaa aatcatttct tttgccataa tcatactctc cttccacaca ttatatacgg
    15901 tactgctgcg gtgtgaactg ccgcatcgat tattatttta ctacaggaac tgttaaaagt
    15961 acagcagaaa ttgataaaaa attaacgaaa tttatttttt atcaggaact ggaaaagtgg
    16021 gtggacgcgc cggggggcgc gtctcaggta cacaaactgt gaaaaccgga tccggacaaa
    16081 atctggatca aggtttatgg aaattagaac cggaaaatcg ccgttccata tccacctaac
    16141 ggataagaaa tagaatatgg ctgtccgtca cattcactct ttacggatgt ataagtaggg
    16201 cactggctgg gcttataagc accgtgaaca ttatccagaa ggagtgacag cttgcagcgt
    16261 ttcggtacac ctacacggta atccgggcga tccactggtg tgaagtttac tacaaacagc
    16321 aggtttttct ttcctgtctc atccttgcgg ataaagctga aaatgctgcg gtccccgtca
    16381 tttgcattga tccactggaa gccgttccag tcgttgtcaa gctggtacag acaaggatat
    16441 ttcttataaa tatgaagaag atcacggaca tattcctgaa gctttttatg gctttcttct
    16501 tcagctaaat accagtcaag gctgactttt tcatcccatt catgccactg gccgaagtcc
    16561 tgtcccataa ataaaagctt ctttccggga tgacctaaca taaaggtata accggccttt
    16621 aagttggaga acttgtcctc tccaagccca ggcatcttat tgatcatgga acacttcaga
    16681 tgtactactt catcatggga gagtactaaa atataatttt cactggtaaa ataggtcagg
    16741 ctgaaggtca tgcggttgtg gttgtatttg cggaaatacg ggtccaattt catgtattcc
    16801 aggaaatcat gcatccagcc catgttccat ttaaaggtaa agccaagtcc gtcattttcc
    16861 gggtggtcgg tgactttcgg ccatgcagta gactcttctg cgatcatggc aacaccgtgg
    16921 ttgcggccct ggacaacggt atttaagtgt ttgaagaatt caatggcttc caagttctgg
    16981 tttccgccgt ttttatttgg cacccactgg cccggttccc ggccgtagtc cagatacagc
    17041 atagaagcaa cagcgtctac acgcaggccg tctacatggt attcttctac ccagtagaga
    17101 gcgttgctga taaggaagtc ttttacttca tttttgccgt aatcaaagac ttttgtgccc
    17161 cagtccgggt gctcgccttt gcgtggatcc tcgtattcaa aaagaggatg tccgtcaaaa
    17221 tcagccagtc catgggcatc tttcgggaaa tgggccggaa cccagtcaag gataacgccg
    17281 atgccatttt tatgtaaata atttacaaaa tacatgaagt cttttggact tccgtaacgg
    17341 gatgtagggg caaaataacc ggtcacctga tatccccagg agccgtcata tggatgctcc
    17401 gcaataccca taagttctac atgggtatag cccatttctt ttacataggc agccagctca
    17461 tgagccgctt ccacataggt ataatatccg tctttttctt cccggtcttt cttcttccag
    17521 gaacctaagt ggacttcata aatgctgatg ggctgtttgg tcatatctgc ctgggaacgc
    17581 tttgagatcc agtcactgtc attccaggaa aatccgctga tatctgcggt aacggaagca
    17641 gtacccggac ggtattctgc gctgaaggca aatggatcag ctttgtaaag gatctttcct
    17701 gtatcggtgg taatggcgaa tttataaagc tctcctacct gcatacctgc attaaaatac
    17761 tcccagatac cggattgtcc tactttggtc ataggattgg cttccgggtt ccagccgttg
    17821 aaatcgccta ccaggtggac tgcctgtgca tgtggtgccc atacgccaaa ataaatgccc
    17881 tttttgcctt tataggtcat aggatgagcg cccagctttt tatagatctc gtagtgagta
    17941 cctgcgccga ataaatagca gtccaggtcg gagatcaggc cggtgttggc ggaagctttt
    18001 ttagaaggtg tgctttttgc tgcagttttt gttccagcgc ttcttctggt ggttttatga
    18061 gctgcggtag ttgtattttt ctttgtctgg cctgcctgct ttctcattgt catattaatt
    18121 ttccccctta attttaagga cagcacccca gttgcccttt aatttaattt ccagtctgcc
    18181 gttttgtaca ggcagaacgt tattttcaca ggtttcatta tcgctgttta aaagatccac
    18241 tgctgtctgt gcctggatcg gaaccgggat gtttaatgtg gcttcattgt catcattatt
    18301 taaagcagtg atgatcaggc tgttatttcc ccatcttgcg taggcatact gcctgttggt
    18361 aagaagcagt tcctggtatt ttcctgtgtg cagttcttca ttttcttcat ggatctttcc
    18421 aagtctggaa atgaaatcgg taagctcgca gtttaattcc ggtatttttt cagccggaat
    18481 gcagggacgc aggtcatcat cacaggtaga agtacgtttt ccttcggtgc cccattcacc
    18541 gccgtagtaa atagacggaa ttcccggaag agtcattaaa caggtgtata caggatacag
    18601 atgctctttt acctttaatt tgctggcaat gcggtcttcg tcgtggttgt caaggaaggt
    18661 ataaagctgt cttccaatgg cttccagacg gcggacatta tgtgcgatct caaagaagtt
    18721 gtggtcatta aagccggagt aaatgctctt gtgcagctcg tagttcgtaa cagaatggag
    18781 catttccggg ttcacccagc ggctgtaatc accgtggatg acttcaccca taagccagaa
    18841 atccggtttc atggaagcgg tctttgcacg catttccttc atgaaattaa agtccaggat
    18901 attggcgcag tctaagcgga tgccgtcaat gtcaaattcg tccacccaga aacggatcac
    18961 atcaaaaaga taatctttta cctgctggtt ataaagattt aaacaaggca gttcaaaatg
    19021 tccctgccat gcttcatagc caaatggatc ccctaaagga ctctgccagc cgaaatttac
    19081 ccctttgtac cagtctttgt aaggggagtc ccatttcttt tcctggatat ctttaaaagc
    19141 gaaaaactca cggcctgtgt ggttgaaaac accgtcaact accactttaa tgccattttc
    19201 atggcaaagc tttgtaaatt cgcggaaatc atcgttagtt ccaagcctac ggtcaaccag
    19261 tttgtaatct ctggtgtcat atccatgact ggatgattca aaaagaggac ctatgtagat
    19321 ggcattgcag cctaaagaac ggatatgtgg gatccactga tccagttctt taaaccggtc
    19381 tgtcacctct gtctgcacgt ttatctttgg agctccggtt atccccaaag gatacatatg
    19441 ataaaagata gcttgttcat accaggtact catgttgtca gtcctccatg tttttgaatt
    19501 ttctcagtaa aaactgatag cgcagctgcg cggcattcca ttcgtaaata tagcagtcga
    19561 tcagcgacgg ctctgtggcc tgctcaaaat ggttctgggc tgtttccagg gcccagaggg
    19621 tgcgctcaat atcatcccac agtttccgtc ttgtaaaaac cagatcctgg tttacaggtg
    19681 ctgcggctgc agcttcgcga atggctttta aaaatcccgg cataaatctt catctcccaa
    19741 cttctgactg gtttactttg gcatcaatta gtatatctgg gaaaaaaaga attatgcatg
    19801 gattacatga actcctccag tctgtatccc agctccataa gagaccgggt ggagtcacgt
    19861 aaggtgagat agtagtaatt ggctgtcccg tcctgacgta cacctaccag acccgcatct
    19921 tttaaaattt tcagatgatg ggatatggca ggtcgggaaa ggctggtgcg gcgtgtgatc
    19981 tcgtttacat tcatggcatg accttcaaag tcgatggttc cctgtgggtc ctctgatctc
    20041 acggctgctt ctgtaagaag ttccacgatg gaaagccgga ccttatctcc caaggcaata
    20101 aaaaggggca tgcagcaatg aaattgcttc tgcagatcct gtaccgttgt cataaccgtc
    20161 ccctcctttg gtttaaattt ttaaactaat agtattttac cattacaggg catcggggtc
    20221 aatagaaaaa tacctcttga caggaggggg aaagtacggc ataataaggt cacattcttt
    20281 gaaaaatcta tttttcattt ttaattctgt aatttttagt gcgatttttc agcacaggat
    20341 ccagttgtga gtttaatcag atacagggat aagtgggtca ttttgtgagt ttatgttttg
    20401 gtttgggagg tgagaaattc ggtttatggg agtttaagtt atttaagtca gagtgtattc
    20461 tgggggacat tgcttttggc agcatggtct gactggaaat attacaggat ccctaacagg
    20521 cttattattt ttgggatagt gtccgggatc atgtcactgg tttttcaggc tgcggatttg
    20581 gaagcaggga gtaagatccc atggctgctg ctggcactat ccggctttct ggtccgggga
    20641 gtggctgtgc tggttttggg gatattttta cacagcctgg gcatgatagg agccgcagat
    20701 ctgaagcttg tggcgcttct tgcagcctgg ctgggctttt cggaaacaat aaaaatttta
    20761 tgtgtaggat ttatcttagg ggctgtctgg tcccttcaaa aaatgttgcg atacggcagc
    20821 gcttttcaac gatttcttta tttgtcagcc tatatcaggc agatactcag caataaaaaa
    20881 atagaaaaat attatgatcc ggtccgggat ggaacagaat gtgtgatccc tttgggaacc
    20941 tgcttttgta tggcaactgt tctggtcttt ctggaaaaat ggatctttta ggaggagaag
    21001 acatgataaa agtaatggtc gtatatgacg aagatccttt gtatgcaggg cgtttggccg
    21061 aatatgtaaa ccagaaggag acatttccct ttcaggcaat ggcattttca gacctggaaa
    21121 aactgaaagc ctatggcagg gaccatgaga ttgccattct tttagtggga gaaagagtaa
    21181 gagaggaagc aaaggagata aaagccggat taaaaatgct tctctgtgac ggggagtttg
    21241 tgtcacagga ggaagcctcg gtttataagt tccagtccgg tgactgtatc ttacaggaag
    21301 tgatggcctg ttactgtacc gttcccccgg agccgggact ggcacttata ggaaaaaggg
    21361 cactgatcat gggagtttat tcacccatag gacgctgcgg aaaaacatct ttttctttaa
    21421 cactggccca tatgcttgga aagagccagg gcgttctttt tatcagctta gaagaatatt
    21481 ccggtttttc aaagctggtc tgcggtggat atgaacagga tctttccgat gtgttttatc
    21541 tgtacaggca gggagatttt aactggctga aattaaaatc cctgatcctg tcccatggaa
    21601 atgtagatta tatcccacct gccgcttatg gggaagattt agaccaggcc cagccggaag
    21661 agatcgcagg tcttttaaaa cagatcggga cagaaagcgg ttatgaacgg atcgttgtgg
    21721 atatgggaca catgggaaaa gggtctttgg agctgtttgc agcctgtgat gttgtgtaca
    21781 tgccggtgtt agatgatcct gtatccactg cgaagttaga ggattttgaa acctatctga
    21841 aagaagcagg ccataaaggc atggaagaaa agatacagaa actgcatctg ccggaaataa
    21901 aaacagggat ccggcggggc ggctatatgg agcagcttct ctggagcgaa atgggcgatt
    21961 atgtgcgaaa acttttagaa gggggatatt aaaaattgga aggggaacag ggcagtttaa
    22021 acggaaggcg ggaagtgatc cggggagagt taagaagaaa tgtcatgggg cagttaaaag
    22081 atcagatcta tttagaggat gaacagcttt atgaacagat cgatacagcg atccggcaaa
    22141 aggggaaaga aacttatctg cctttaaagg aacggctgtg gctgcgaaac agtctgtatg
    22201 acagtttccg tcggctggat atcctccagg aacttttaga tgataaagat gtaaccgaga
    22261 tcatgatcaa cggggcggga aagatcttta tagagaaaaa gggaaacatg gtcttatgga
    22321 accgccggtt tgaaaaaaca gagcagttag aggacattat ccagcagatc gtaggaaagg
    22381 taaaccggat cgtcaatgta tcttctccca ttgcagatgc caggctggag gacggatcca
    22441 gagtccacat tgtactgccg cctgttgcac ttgacggtcc ggtggtgacc atccgtaaat
    22501 tcccggatcc cattaccatt gagaagctga tccgtttcca ggcactgaca gaggaagcag
    22561 cccagttttt agagcagctg gtaaaagcga aatataacct gtttgtctgt ggcggtacaa
    22621 actccgggaa aacaactttt ttaaatgctc tttcctcttt tataccggaa acagagcgtg
    22681 tgatcaccat tgaagactct gcagagctgc agatcaccca tgttcccaat ctggtgcggt
    22741 tagagacccg caatgccaat acagaaggag aaggtgagat caccatcagt cagctgatca
    22801 aagcctcttt gcgtatgaac ccctccagga tcattgtagg ggaagtaaga ggggcagagg
    22861 ccttagatat gcttcaggct atgaatacag gccatcccgg ttctttatct accggtcacg
    22921 gcaacagtcc ccaggatatg ctttcaaggt tggaagtaat ggtgcttatg ggagcagagc
    22981 tgcctttaga tgcgatccgc agccagatcg cgtcggccct tgatattatg gttcatttgg
    23041 gaagattaaa agacgggagc agaaaagtat tatcgattgt agaaataggg ggatataaaa
    23101 atggaaaagt ggaacagaag ccactttttg tctatgacag agaggaaaaa agacttaaga
    23161 aaacagggga acttaaggcg agggaaaaac ttgctgatgc aggagattaa cagaaaaagg
    23221 tggagcaggt cttctggcag ggaaaagcag aaagaggatt taaacggcag ggcaagaccg
    23281 ccggattaca gggaatatca attatcccaa aaagaatgga tgcaatatct gatcatggga
    23341 gccggggcag caggactttg tgcttataca ttttatcgca gtgtacttgc atttttattt
    23401 cttcttccag tggcttttct gtatccggtc tataagaaaa aggatctgaa aaaagaacgg
    23461 atccgcaggc tgacactgca gtttaaagag gggatcatgg ttttggcatc tttcttaagc
    23521 gccggttatt ctctggaaaa cagtctttcg ttaagtgtaa aagaattgga aggcctttat
    23581 ggaaaaacag ggatgatcac agaagagttt tcctatatgg aagcaggggt aaggatgaac
    23641 cgtccggtgg aactgctttt agctgatatg ggaaggcgca gcggtattca ggatgtggat
    23701 cagtttgccc gggtatttgc agcggcaaaa cgaagcggag gggaactgac ggatattata
    23761 agccagacgg ccgggatcat ccgtgacaag atacaggtac aggaggagat ccatacactg
    23821 acagctgcca gggtatttga acaaaagatc atgaacggaa ttccttttgg gatcatattg
    23881 tatatcgatt ttacatcacc gggctttttt catgttatgt atgaaacctg gctgggtcgt
    23941 atttttatga cagcgtgcct ggggatatat gtgggagcag tatttctggc aaagaagatc
    24001 ctggatatcc cattataagg cagatgaatg tatgaaagaa acgaagagaa aattttataa
    24061 aaagatctgt aagtggaaaa agcagatttt atgtgtggga gccggtattt ttctgggaat
    24121 tgctgcatgg ctggcacagg gcatggatcc tgtaatagaa gcaggaaatg tgatccaaag
    24181 accggatatc gatgatggac aggtggacca ggaactgtat gtaaaggggc ttgtggatca
    24241 ggagaaggaa gtggttttaa aactgcctgt ttctgcaagg cagtatacaa aagaagaagc
    24301 atatcaggca tatgaggaaa tcttaaaaca gcttccggaa tggatcaaag gggacaacct
    24361 ttcactggaa gaggtaaggc aggatctgga gctgcctgca tactggcagg gagcaggagt
    24421 gcatcttagc tggcagtcat cggatccgga actggtggaa agtgatggaa ccgtgcatac
    24481 ctgggagttt gaaacagggg atgaggctat cgatcagtgg ccggtcatct taaaagtgag
    24541 gatgacagat ggaaactggc cggaagaata tgagattccc attaaagtca ggcctccatt
    24601 gtatacagaa gaagaacgta ccatacagga atttacatca cttctcacat cagaggaaga
    24661 ggtacagaaa catcaggatc aggttacact gccctctgta tacaaagaca gggagatttc
    24721 ctacagcaca gccagggaac ctgtatttct tcaaatgtgc tttctggggg cagttgcagc
    24781 ggtatttata agcctgaagg aaaaaagcga caaaaagaaa gcagaagagg aacggaaaga
    24841 ccagctgctt atggattatt ccgaggtgct gtcacggctg atcattttcc taggggcggg
    24901 catgagtata cgcactgcct gggaccggat cgcagaggat tataaaatgg ctgtgaaaga
    24961 aggacgcaga gggctgcgtt atgtatatga agaaatgtat gttaccagca gtcagctaaa
    25021 aagcggggag tcagaagcac gtgcttttgc ggaatttgga aacagatgcg ggctgcagca
    25081 gtacaggaag ttttccgggc ttttagagca gaatcgaaaa aacggctcta aaaatttaag
    25141 ggaaacactg cggttagaaa tggcagatgc ctttgagcag cgtaagcacc aggcaaaacg
    25201 tatgggagaa aaagcaggta caaaattgtt gatcccgctg tttcttctcc tggctgtggt
    25261 gatggcgatg atcacggttc cggcatggat cgcgtttggt taaaaggagg atatgagatg
    25321 gaaaagcaaa gagataatca tatagaacag aatgtaacgg aacaacatac aacagagcag
    25381 ggcggaattt atagaaagat cagggatttt gcgctggatg aaacaggtgt gggggtgata
    25441 gaacttgtgc tcatcctggt ggtcctggta agtcttgtga tcgtttttaa gaagcagatc
    25501 aacacacttt tagaaaatat cttcaagcag atcaatacaa aatcaaaaga ggtttattga
    25561 tgagagaaaa gggagaagtt accgtcttcc ttgcgatgat cctggtgatg atcatgacac
    25621 ttttgctggt tatggcagag tcagcccgta cagcggggca gcggctgtat ctccgagtag
    25681 cttcaaattc agcaatggag tctttgatgg cacagtatca cagaagcctt tggaatgaat
    25741 acaggatcct tggactggaa acggacagta aaggcctttt agaggaagaa tttaagggct
    25801 ttctggaacc ttatatgaag gcaaaaaact ggtatccgtt gaaaacagag gatgcagttg
    25861 taaaagacat ggcagtgctg acagagggaa aaggcagctg tatggagcag gagatccttg
    25921 attatatgaa atacgggctg gcaggtatac tctgggagtc tatgacagaa ggggaagcca
    25981 aagaagttct gggggatata aaaaatgcgg ccagtgtgaa cagtgtgtca gatctgtatg
    26041 aagaacacag caaagaagca gttgcccttg aaaaagccct tgaaaaatta aatgccagac
    26101 tggaacagca gaaaacacac tgggaagaag gactggactg cctggaaagt ttaaacggcg
    26161 aaggcatgat ccgaaaatgt gaagctgtga tcaaagacct gaaggcagtt ccgggactgg
    26221 tggaagctta tgaaaaaaag gcagaccaga tggaacgcgc ccttaaaaaa agcagggaga
    26281 aatttgtatc cgaggaagat ctaaaagagt cttcccgggg acctttaaca aaagagatcc
    26341 ggtcttatga aacctacatt tcccaggacg gagaacgaag aaacatgatc cggggactta
    26401 cagaaaaaag cagggaaaat atccggttta tggaacagct gatagaagag gcagaagaag
    26461 tgatccagta tattgacgac tgggaacctt ccgatgaaga ggatgatctg gatgaagaag
    26521 aactgtggga accggtgatc cgccatatga gccggtatcc ggttctgaca ttgggagttt
    26581 cttttggagt aaaagacaaa gaaaaagagg gatggttaga gaaaatcaaa tcaatgagtg
    26641 gaaaaggcat tttgaagata gtattaccgc cagactgcca ggtttcagaa aaaagactgc
    26701 ctcttttaca ggcaccgtct gctctttcaa aaaatgacac tgcacatttt caggggattt
    26761 ccagcttgat ggatcgttta acagtatctg agtatggggt gcggtacttt tcccattttg
    26821 aaaaaataaa agacaaggat aatttttacg aacttgaata tattttatat gggaagaaaa
    26881 cggacaggga aaacctggag gcatctgttt taaaactggt ttctgtacgc cagggattga
    26941 atatgatcca tatattatca gatggaaaaa aacgtcagga agcagaaacg ctggcagcgg
    27001 ctataacagg aggtggtagt ttgcttccgc tgacagcagt ggtaactttt tttgttatgg
    27061 gtgtgtgggc tttggcagag gcaatggcag atgcgagaag ccttttagac ggtggaaaag
    27121 tacctcttat gaagaccggc tctgactgga aactgagcct ggaaaatgtt ttgaaaacag
    27181 gagcagaagg aagacttccg gatcttgaaa aaacggatcc tgaaaatgaa atgggactgg
    27241 atctgaaagg atatctcagg ctgtttttat ttgtaagtta tggtacgcag cctttattcc
    27301 ggatgatgga tataatgcag atcaatatcc gcagggaaca gccggacttt ttgttagaac
    27361 aatgtgtctg tatggtggat atggaagctg tattttgtgg aaaacctgta tttttttctt
    27421 caggtccgtg gaaaactgcc ggaaagagcc gctttgctgt aacgggaagt tatctttctg
    27481 ttccctgaat gacaatgtat ccggacatat tccgggagaa tattacaaaa gaaaggaggc
    27541 ctgtgatgat gccctttttc aggagcaaat cttattctaa ttatttaaca cgacactcca
    27601 tttataaatc aaaaaaattt ttcgcacctc tccaagtacg aacccataaa tatgtagcac
    27661 ttttaaaaca gaaaaaattt tccggaaaaa gggcattatc atgggtctcc tttggaggac
    27721 ggagaaacat tacagaggaa aaaagaggaa gtcttacatt agaagctgcc ctttgttttc
    27781 cgctggtggt atttttctgc gtgatcctga tcatgcctat gatcctgatg gacaggcaaa
    27841 ggcagatcca ggcagcagtc gagtctgtgg gagaagagat cagccagtat gcctatgttt
    27901 cttatcttta taaatccgga aagggagaaa cattagatgt cagtggcaca aacgagcggg
    27961 aaggtagtca gcttcttgaa aaagtaacag ctgcatatgc tgttgagtca gtgaaaagaa
    28021 agatttcgga gcaatgggtc cgggatctta gtttttatgg gacgcagatc acggaagatg
    28081 aaatgcttca gatcattatg aaatataaaa tgccactgcc attttccctg tttggccagg
    28141 atagtgtgga tgtggaaacg gtttgcagcc gcaggttgtg gacaggggca gatggagaaa
    28201 gaggaaaaaa gaaggcagaa accggggaac aggatgaaag aatggtttat ataggaaagt
    28261 cctctacccg gtatcatttg caaagcacct gccattatct ttttcacaac ctgacccaga
    28321 tccattcaga tgatcttgta agtatacgca atcaggatgg aaaaaaatat aaaccctgct
    28381 ctgcctgtaa aagcagtgaa aatgccggtc cgtattatgt ccttccctat gggaccagtt
    28441 atcacagcag ggaagattgc cggtcgctta ctgcatatgt acaggaagtg cccttgcggc
    28501 aggtggaata tttaggcccc tgctcgtact gtgcgggcgg aaggtaaaaa tgaaaaggag
    28561 aatacaaaaa tgaatatgaa aatggggata gaagcagcac tgaggatctg ttttttatta
    28621 tttttactgt cagcttcctg gcaggatcta aagaaaaaga ctattaaaaa aagtacgttc
    28681 cagatatggg gaacggtagg ggtcattctt caggcagcac atattttcag ccagattttt
    28741 atgggaacag aaaaaatgga aatgcaggtg atcgtacagg aagttacggg ccttatgttt
    28801 gcagctttat tgggaatttt gttattaagc ctttctactg ctacggaaga agctatggga
    28861 aaaggagatg gctgtttctt tctggtagca gggatcttcc ttggtttctg gaaaaatatg
    28921 ctgctgttat ccggcggtct gtttttatgt ttcccggcag ctgtttatct gatgataaag
    28981 ggaagaaaag cgagaaatac agaaagcatc ccgttcttgc catttgtgtt tccggtggga
    29041 ctgggggtcc tgtttttatg aataaaagag gggaaactgt attaaaagat gaaagaaaaa
    29101 agcgaaaatc catagaaaca gaatggaagg gaagccttac agtagaggct tcctgtgtta
    29161 tggctgtggt gctgttttcg ctggcagccc ttataggaaa agcagggcag atccatgatg
    29221 aaacggcagc agctatggtg cttcacgaag gtgtagagaa atgccgacat gaaaaaagta
    29281 taagatcaga agatgcagaa gcattttgca aaaggaatgc agggctgatg ctgcggtata
    29341 cggatctttc tgtttccata caggaaaagg gagcaaaaaa gatgggaaaa gtgaaaggag
    29401 gggactggga aaaacagatt gagatgaaag aattcagacc ggaagaattt atgcggatgg
    29461 tcacagggat aaccgggggt acaaatgaga attgaatata tacgggaaat gaagcgcagc
    29521 tatatgacag taacagggga aggagcgggg gaaagctatg aaaccggcat gctggctaaa
    29581 aaccagatct ccggtcttct gaaaatgaag ataaaataca tggatggagt acctgtatat
    29641 tgttatgata ttacttcccg ccagcctgta agccggctgt ttgaaagccg gcctgcaggt
    29701 gaaaaccagg tgagaagttt ttttctgcag ctttatgaaa cattagaccg gatggaagcg
    29761 tatcttttag gtgatggagg gatcctttta gatccggaac tgatctacgc agatccggaa
    29821 acctttcaga tgggcttttg tgtggtgcct ggaagaaagg aagattttaa cagccagttg
    29881 tctttattcc tgcaatatct tatgaagaac atagatcaca gggacaggga atgtgtggtt
    29941 cttacctatg gactttacca gaccagtatg aaagataatt atgggatgga agatatgatg
    30001 gcacttcttt ctaaggaaga ggcaaaagat ttaaagacgg aaaaagattt gcttatggaa
    30061 cggaaaggag aaggaaaaac agaaacaaga ccagaaataa aaacagagaa agaagatatg
    30121 gcagaagagt catctttgta tgggcttgaa aatgatgcac aggaggaacg ggagctattt
    30181 tctgaagaaa gaacgttcgc aaataatgga tggagcctgt ggaagaaact gttacttctt
    30241 tttttaactg cggttatggc agcaggcggt acctggtttc tgtatggctg gtctgtatta
    30301 agaaaaatgt gggtctatgg ggctgcaggg tttggaattg caatattagg aaccgtttta
    30361 ttgcatatga tgcgaggaaa aggcaagggt ttgttgcctg gaaaatatgg aggaaccggc
    30421 gcaaaagagc cggaaaattc ctggcaggta ttttatgaag agacagaaga agcgcagaat
    30481 gcagagaaag cagaagaaat aagacaggga tcagcgccgg aaaaaatacc gggatatgta
    30541 cctgcaagag aaaagacagc agaaagacag caggaaaaac aattagagga acctcaggaa
    30601 gaatgtcaga ccatgctgct tactgtaaaa gaggagccgg atcatatgct ggtatcggca
    30661 ggagaagaaa atgaagatat tgcagtggga tatgttccct ttgtgatagg aaaacataca
    30721 gggctggcgg actatatttt aaataaaagt acagtaagcc gttttcatgt aaggatcaca
    30781 gaggaggatg ggcagtattt tcttacagat ctgaattcca caaatggaac aaaggtcaat
    30841 ggaaggctgc tggcagccaa cgaaaaagtg gtgctcagtc agggggatga agtgtggatc
    30901 gcagaggaaa agtacctttg gagatagaaa aagtcataaa acgacaggag gtgacagaaa
    30961 cagacatgga gaggggacgt ttttttggag atattgcaga aatgcgctta acaagggaaa
    31021 aaaattatgc tagtattata aacaagaaaa gcgcttatcg acacagaaga ggaggaatta
    31081 aaaatggcag aagataattg tattttttgt aagattgcaa ggggggtgat tccttctaca
    31141 actgtttatg aggacgagga tttccgtgtg atcctggatc tgggtccggc atcaaaagga
    31201 cattcactga tcctgcctaa aaagcatttt gccaatgtat gtgagctgga tgcagagaca
    31261 gcggcaaagg tgcttccact gggagcgaag ataggagctg ctatgaagaa ggggttaggc
    31321 tgcgccggat ttaacctggt tcagaataac ggtgaagcag caggacaaac tgtaatgcat
    31381 ttccatatgc atgtgatccc gcgttatgag ggcggtcctg ccaatattgc caactgggta
    31441 ccgggtacag catccgcaga agaattaacg gaagcagcag agaagatcaa aggctgtctg
    31501 tagtcactta tgatcaaacg tatggatgaa gaagcaaaag cgagttttga agaaatgtat
    31561 ctgacctatc aggatacctt aagacgtctt gcttatgctt acgatatacc agtggatgat
    31621 attgacgata tcattcagga cacatttgtt tcctatgcaa ggtatgatta ttccctgaaa
    31681 caaccggaag aggggaagaa gatcttgctt ggcaggatcc ttagaagccg ttgtatggat
    31741 tttcacagac agaaaaagaa acgatattgt gaagatctga atggagaaac gtttgcagcc
    31801 gaagagtaca ggatgcagct gcatcagcct gggttagtag acgttttctc cggaaaagag
    31861 cggtgcaaag cgatcctgga cgaagtggac cgtatgccgg aaaactggca gaaagttgcg
    31921 gagttgaaat taatagaagg acggccaacc agtgaagtct gtgatcagct ttctatttca
    31981 gagaaagcct gctattcacg ggtcagccgt atcagaaagt acataaaatc acttttgaaa
    32041 cgggaagact ggccctagac aggaccatgt tctcccgaaa caaagggaaa tccgttgctg
    32101 gccatgcaat gcatgaacag taacaattta agaatgggaa acgcaggact ctatcaaaga
    32161 ggtaatggtt tctaccgcat tgctgagact gcttttttcc attgcattga tataggtttc
    32221 cctgtttttg taaagatcaa acacagcagt gcttaaggtt tcatctgaaa gttcttcttc
    32281 ttttaagact ttgctgaagc cctggcgtgc gaaggaatct gcatttaaga tctggtcacc
    32341 gcggctggca gcagcagaaa gcgggatcag aaggttgggc ttacgtaaag ccagaagttc
    32401 gcagatggaa ttagcacctg cacgggagat gacgacatca gcagcagcaa acagatgctt
    32461 taaaggtgca tctacatatt catactgtac atatccttcg gtaccgatca ggctttcatc
    32521 cagattgcct tttccgcaga tatgtattac ctggaatgta tttaaaagct gtggaaggat
    32581 ctttcgtacc gctgtgttta cagcaacaga tccaaggctt ccgccgatga tcagaaggac
    32641 aggtttatct tctgttaaat gagcgtagga caggcccgct ttgcggtcac cttctaaaag
    32701 ttctgcacgg ataggagaac ctgtaagaac ggctttgtct tttggaagat atttcagtgt
    32761 ttccgggaaa ttgcagcaaa cttttgcggc agatggaata cagatcttgt ttgcaaggcc
    32821 gggagtcata tcggattcat ggataataac gggaacctta taatgttttg cagccagaac
    32881 gactggtaca gcaacaaaac cacccttgga gaatacaaca tctggtttat aacgcttcat
    32941 aaggcgaagt gcctcaaaat agccttttgc tacacggaaa ggatcagaaa aattcttcag
    33001 atcaaagtat ctgcgcagct tgcctgagga aatgccgtcg taaggaatgc cggcaccttc
    33061 gatcagtttg cgttcgatac cctggtaaga accaatataa cggatttcat agcctgcttt
    33121 ctttaaagaa ggaagaagag caaggttggg agtaacatga ccggcagtac cgccgccggt
    33181 taaaatgatt tttttcataa aaacctcctg agggaaaaac atctactaat atagtaagca
    33241 ttaactgcaa aaagtcaacc ctaaaccgag agggaactta aggtatttga caactgaggt
    33301 gtaaagtggg aaaaacagga agattgaact tggaaatgaa tgactggatg ggaaatcaaa
    33361 ataaaaagag ggaaaaactg actgacagtg atattttaaa gcaatttgaa caggcagaaa
    33421 aagatacccg ttttttgcct atcaatccgc cgccaaagga tgagtttgac cagatctggt
    33481 cacggatcca ggcggagcag gctcttttct cagagatttc caatacaacg aataccaaat
    33541 catcccgtaa aacccgcagg cgccctgtaa gaaaagcact ggttgtgggg ctggcagcag
    33601 caattatggt tattggcgga tgctttgtgg ctatgggaac gaaatcttat ttttatcggg
    33661 atggaagaag tgtaaatcag ggagtagttt ataataatga ctctggagct ttgacggttc
    33721 gtagtgaaga agaggcatat gagaagatag cggaagaatt aggggttaag acgctgaagt
    33781 tggggtatat gccggagggg atgaggtttg aaaatgtaat tatagataat ggttatggaa
    33841 gattacaata taattataaa aacagtaagg tactatttgt tcaaactaaa tcagatgata
    33901 aaacgtctat ggtgcattct tcagatgcaa gagaaatgaa tgagatatgg aaccaaggac
    33961 tccatatgaa tatatcaatt tacggtgaga ttattgctaa gaatagaaaa aggtatgaag
    34021 cgtcgttttc gtgtaatggc gtatattatt atctttttag tgaaatgcaa caagatcaat
    34081 ttgaggagat gttaaagaaa gttcattttt aagagatagg agattgtatg aaaaaagtaa
    34141 tacggttgtt ggtgagtgtt ttagcaatag ttaacatatt tgtaggaaca gcatttgcgg
    34201 gaattccgtc gccagaaatc tatacttctg taaaccaaga gtcaaaacta tgtgataaaa
    34261 atatttcata tagtacggga acagcaaagt cttatgcaag aagaggaaga ttttttgtat
    34321 cagctgatgt tgctataaaa gatgaaggaa atggaaatat tggagcatca gcatatgcat
    34381 atttaagtaa acctgtaact gaattatatg tttcaatgtg tctggataga tacaatgcaa
    34441 atatagatgc atgggaacag gttgattatg tggattttga atatacatta gaagaatatc
    34501 ctgatggtat tgaagatcct attatccatg ttacttttga aaaccagaaa cgtggttatt
    34561 attaccgcat cagaggagct ttctccgcag gtgatgatga ttggtatgag ggcttcggac
    34621 caaccaccga tggcatctgg atcgaataaa ccatccaacg caatttaatc caacaataac
    34681 ttacaactaa aataagtaac ataacttaga aaagcagctt cccccaaagg caattcgata
    34741 cacgcaattc cctggcagaa gctgctttct aacatttaag gtaaaactgt cagtggcagt
    34801 tctgcttaat atcatgtata ataactcgat gtaggcaaaa tgattgctct gataccgaat
    34861 tgctacgtgc tttgcactct cgcaattcta aaaacattcg taatccgttc atttttcttc
    34921 aaaaaatggc ttcttactca tgtttttggc gggccccaag gtcaaaactc cttctgcaag
    34981 caagcttgcg tcggatttcc gaccttttga ccctggtatc ataatattac atttcataaa
    35041 cattgaggag aaatataact atgcgtgatt atttaaacag actcagacag cagtttcagc
    35101 gctttatgat cggaagatat ggaatggacc agtttgggcg gtttctgtcc ggtgtgcttt
    35161 tagcgttgat catctttaat ttactgatcc cggcggctct gccttctaga atattatggg
    35221 cactggaatg gatcgtgctc attatattgt atgcaagaat gttttccaaa aacattaata
    35281 agcgttatca ggaaaatgaa gcctttttaa gacggcagtt ttatgtatca gaatggtgga
    35341 agaagtggaa attccgctat caggaaagca gaaagtatca tatttaccgt tgtccgaact
    35401 gcaaacagaa agtgcgtgtt ccaagaggaa gaggacggat cagtatccac tgcccgaaat
    35461 gcgggactga ttttatcaag aaaagttaaa aaagactgta tgacaagacc ggctgtatgg
    35521 aagctggtct ttttttgcga ttttatagcg catttttgta cgtgcaatct gaccggttta
    35581 cttaaatttt acaaaaccta taccatacct tgctttcagc tattgtaaaa cttttgtata
    35641 ctccacaatg taagaacaag aaaacaacaa agaaaaatat aaataccgca aaacaataca
    35701 aacataaagc ggtacaaaag tggaggaaaa gataattatg aaaaaaaatg gcatgaaagt
    35761 aatggcaatg atcggttctg cagtaatggc agcaggtatg cttgcaggat gtggaaactc
    35821 cggtgcagca gctaccacag cagctccagc aactacagca gagcagacaa ctgaagcaaa
    35881 ggcagaagaa acaacaaaag aagcagcaac agaagcaaag agcgcagatg cagatttaag
    35941 cggatccatc agcatggtag gttccacttc tatggagaag ttcgcaaatg cattaagcga
    36001 agcatttatg gagaaatatc caaaggtaac agtaaccgct gagttcgtag gttccggcgc
    36061 tggtatcgag gcagtttcta acggaacagc agatatcggt aactcttcca gaaacttaaa
    36121 agatgaggaa aaagcaaagg gagttgcaga aaacatcgta gcaatcgatg gtatcgcagt
    36181 tgtagttgat cctgcaaaca cagtagaaga tctgaccaag gatcagctga ccagcattta
    36241 tgacggaaca gtaaccaact ggaaagatgt aggcggaaat gatgctccaa tcgtagtagt
    36301 aggacgtgag gcaggttccg gtacaagagg tgctttcgag gaactgttaa agctggaaga
    36361 tgcctgcaag tacagcaatg aattagacag caccggcgca gtaatggcaa aggtagcatc
    36421 taccccaggt tccatcggat atgtttccct ggatgtatta gatgacactg taaaggcatt
    36481 aaaattagac ggcgctgagc caaccgaaga gaacatcaag gcaggaaagt acttcttaag
    36541 ccgtccattc gtaatggcta ccaagggcga gatcagcgag cagagcgatc ttgtaaaggc
    36601 actgtttgac ttcatctact ctgacgaagg ttctgaactt gtaaaatctg taggtctgat
    36661 cacagctgac aaataatcag tgaacagata acacgaaaaa gaaataaaga cgataaaaag
    36721 tgtgagtcat acaaaacgaa aggagactgt ctgcaatgac acaggaaaca ttttccgtcc
    36781 gttcccatca tggcagcaag tctacagtag aaagagctgc agaagcgatc ttcaccgcat
    36841 gcggcttttt cgctgttctg gctgttgctt ccattacatt atatatgata ttcagcggta
    36901 caccggcgct gtttaaagta ggcatcttgg atatcctttt tggtacctta tggcagccgg
    36961 ctgctgctac accaagtttt ggtatcctgt atgttatcct tacatccatt gtgggaacct
    37021 ttctggcgat cctgatcggt gtgccggttg gcgtgatgac agcagttttc ctggcagaag
    37081 tagcgccgaa aaaactggcc aatgtagtcc gtccggcagt agagctgtta gcaggtatcc
    37141 cgtctgttat ctacggtctg ttaggtatcc tgatcttaaa tcctctgatg tataaaatgg
    37201 aactggctgt atttaaaggt tcttctactc atcagtatac cggcggcgca aacctgatct
    37261 ccgcagtgct ggtactggcg ctgatgatcc tgccaaccgt catcaacatc agtgagtctg
    37321 ctttaagagc tgttccgggt catttaaaaa gcgcttccct tgcattaggc gctacaaaga
    37381 tccagactat tttccaggtg atccttcctg cagcaaaatc cggtattatc acagccgttg
    37441 ttttaggaac aggaagagcc atcggcgagg caatggctat tacactggta tccggaagtt
    37501 ccgtaaactt cccccttcca tttaactccg tccgctttct gactactgct attgtagcag
    37561 aaatgggata tgcatccgga ctgcacagag aagtattatt taccatcggc cttgtattat
    37621 ttgcatttat catgatcatc aacattggtt taaacagtat tctgaagaag ggagagaaaa
    37681 caaaatgaca ggtgaagccg ctatccgtat caatgaaaac tccgtgatcc gggaaagcat
    37741 ttatggaaaa cgcatccgca gcacagatgt gatcttaacc ggtattatct acggatgtgc
    37801 cagtctctcc atgctccttc tcatcggaat tttaggttat acctttgtaa ggggtgtccc
    37861 acatgtgacc tggtcctttt taagcactgt ttccagcgct acaaagggaa cctttggtat
    37921 ccttggaaat attatcaaca ctctttatat tgtagtgatc actttgctga tcgcaactcc
    37981 tattggtgta ggttctgcgg tttacttaaa tgagtatgca aaaccgggaa agatcgtccg
    38041 tctgatcgta tttacaacag aaacactttc cggtattcct tctattatat ttggtctttt
    38101 cggaatggta tttttcggaa atacactggg actgggctac tccattctta ccggttccct
    38161 gacactgact ttaatgatcc ttccactgat cacaagaacc acccaggaag cattaaagac
    38221 cgttcctgac agttaccgtc acggtgcact gggaattggt gctactaagt ggtatatgat
    38281 ccgcaccatc cttcttccaa gcgctatgcc tggtatcctt acaggtgtta tcctggcgat
    38341 cggacgtatc gtaggtgagt ctgcagcttt actgtttact gcaggaagcg gctattacct
    38401 tccaaagaac ctgtttggca aggtacttga gtcaggtggt acactgacta tccagctgta
    38461 tctgtttatg cagaaggcaa aatacaatga agctttcggc gtagcagttg ttcttctggt
    38521 gatcgtactg gcgatcaacg cactggcaaa atatctgtcc caccggttta atgtagagac
    38581 aagataaacg tgaaagacgc gaaagggaaa tgagtataat ggaaaataaa gtaaagatct
    38641 ccacacagaa cctgaacctg tactatggag aaaatcatgc actgaaaaat attaatctgg
    38701 acctttatga aaataagatc actgcattta tcggaccatc cggatgtgga aaatctactt
    38761 atttaaaaac attaaaccgt atgaatgacc tggttccaaa tgtaaagatc gagggaaccg
    38821 tgcttcttga cggggaagat atttatgatc cgcaggtaga caccacactt cttcgtaaaa
    38881 aagtgggaat ggtgttccag cagccaaatc catttccgat gagtatttat gataatgtgg
    38941 cttacggccc aagaattcac ggtatcaaga acaaggcaca gttagatgag atcgtagagc
    39001 gcagtttaaa gggcgcagct ttatgggatg aggtttctga ccgtttaaag aaatctgcac
    39061 tgggactttc cggtggtcag cagcagcgtc tttgtattgc aagagcgctg gcagtagagc
    39121 cggaggttct gttaatggat gagcctacat ctgcactgga cccgatttct accttaaaga
    39181 tcgaggacct gatggacgat ctgaagagca agtatacggt tgctattgtt acccacaaca
    39241 tgcagcaggc aacccgtatt gcagattata cagcattttt cctggtaggc gaggtagtgg
    39301 aatatgctac cacagatgaa ctgtttacca tgcctaagga taagagaacg gaagattata
    39361 ttaccggacg ttttggttga tggaatgtga tcctacggta aaatgtgaga atgtagagtt
    39421 gcgaaaatgt tggaaaagca acggaaaggg gcataaggga tgcgcattac ttatgaacat
    39481 gaattagagg agttgaataa atgcctgcgt gatatggcca tgatggtgga aaaagccatt
    39541 gagcagactt tcgtggcatt tgaagaccag aattatacca tggcagagga tgttattaaa
    39601 ggagaccgga atgtaaatga catggaacgt gccattgagt cccgttgtct gtccctgatc
    39661 cttcgccagc agccggtagc aagggatctg cgccagatct ccactgctct taaggtggta
    39721 acggatctgg agcgtattgg agatcatgca tctgatattg cggagctgat cctgcggatt
    39781 aagagtgatc atgcatacca cattgtacag catcttcctg ctatggcagc gtcagcccag
    39841 aaaatggtcc atgatgcagt agaagcattt attaagcagg acgtggaaga ggcagaggag
    39901 atcatccgca gggatgatga ggtggatgcg ttgtttaatc aggtaaaatc ggatgtggtg
    39961 gagctgttaa aatctgcgcc gtcccatgca gaccagggga tcgacctgtt gatgattgcc
    40021 aagtacttag agcgtattgg ggatcatgcg gtaaatgtat gcgaatggac ccagttctgt
    40081 aagaccggtg cgctgaaaaa tgtgcgtatt atgtaagggg atttagatgt aaacacgaaa
    40141 gagtccgcag gccgggctct ttttggtgcg tttttatgca tgatattcaa aaatataatg
    40201 cggtattttg cacatatagc cataaaatgt ggcaaagtaa tgcggaaatt tgcttgacat
    40261 gtgcatggtc tgcgatataa tacaagtata aaatgatacc ggggtaaaaa cacgtagcaa
    40321 tccggtatcg gtgggaagca aaatgtaaga ggaggactat ttcatga
//
LOCUS       DBMDUA010000005        39246 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_870_length_39246_cov_4.429046, whole genome shotgun sequence.
ACCESSION   DBMDUA010000005 DBMDUA010000000
VERSION     DBMDUA010000005.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 39246)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 39246)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39246
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_870_length_39246_cov_4.429046"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..85
                     /locus_tag="ACZPCL_00840"
     rRNA            <1..85
                     /locus_tag="ACZPCL_00840"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            176..249
                     /locus_tag="ACZPCL_00845"
     tRNA            176..249
                     /locus_tag="ACZPCL_00845"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:210..212,aa:Asp,seq:gtc)
     gene            281..353
                     /locus_tag="ACZPCL_00850"
     tRNA            281..353
                     /locus_tag="ACZPCL_00850"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:314..316,aa:Val,seq:tac)
     gene            396..477
                     /locus_tag="ACZPCL_00855"
     tRNA            396..477
                     /locus_tag="ACZPCL_00855"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:430..432,aa:Tyr,seq:gta)
     gene            530..603
                     /locus_tag="ACZPCL_00860"
     tRNA            530..603
                     /locus_tag="ACZPCL_00860"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:564..566,aa:Met,seq:cat)
     gene            631..703
                     /locus_tag="ACZPCL_00865"
     tRNA            631..703
                     /locus_tag="ACZPCL_00865"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:664..666,aa:Phe,seq:gaa)
     gene            721..793
                     /locus_tag="ACZPCL_00870"
     tRNA            721..793
                     /locus_tag="ACZPCL_00870"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:754..756,aa:Lys,seq:ttt)
     gene            900..2732
                     /locus_tag="ACZPCL_00875"
     CDS             900..2732
                     /locus_tag="ACZPCL_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HIR1161076.1"
                     /translation="MRRRKKDSGLKTALLLIVAIIFMIMAISVIVIVFGKIHSDYEKL
                     DMTRASEKQTLEISAAETKETDQLGWEETEDGWKYKIDEKTYAVDQWLEIKGFLYHFD
                     DKGIMATGQWKGDGQIFTCHDVKGYLKNIEPDPNYVPEDTGEDMDSFIRTNAFWCYLD
                     SEDTGLFKTILYKKTVDNKVKPLGNEKNPEKATKNSLRAYGDYVYYLPKVAESKRSSL
                     SEEEKERCDVLVRMIPGQNTKEIIAENVDGYLVLDETIYYAQEGKIHTATSGTETELS
                     EGGYQVKIDGDACYLVNALGKPVTSAGSAGITVEDRVYKVDENGKISYVKKNPVAADD
                     SSYELQGTGSQMQLVRRSSSGSKTIARADYGVQSYCIVDNSIFFSAYVEKTADGKWYS
                     RIYKTDLNGKEKKVVSGIFPGAVFNLYYFESQGEIYGEYHPKIWENAYGVVVNVKQDG
                     TIYAVKDTSERTGKKVTGNDMIEILALQDGKMIGYWHDCNWSRTGGITSVLWSLPVSL
                     DVSAKTRVETVEEIKEEETGTSVEETTTTTAEKIVPVTPTHSSVSPVETGKVHPSVNP
                     TEVVIGTDAPGKRVEETTVATAPIAPATTAAETTAEEIKIVPLS"
     gene            complement(2762..3004)
                     /locus_tag="ACZPCL_00880"
     CDS             complement(2762..3004)
                     /locus_tag="ACZPCL_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161077.1"
                     /translation="MRTVEFKMERPGLVSAGDKVKIIEREGTSSYSYIIDPAVAMSGC
                     FAARDRIKSEYGIVKDVRENSRGFYVVVDFDEEDVH"
     gene            complement(3036..3446)
                     /locus_tag="ACZPCL_00885"
     CDS             complement(3036..3446)
                     /locus_tag="ACZPCL_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HIR1161078.1"
                     /translation="MKIVIIDGQGGRMGKAVIEQLKSSYPELELYAIGTNSIATSAML
                     KAGASYGATGENPVIVNARNADLIIGPIGIVMADSLMGEITAAMATAVSSSSAYKILI
                     PVNRCNHFIAGCQGGSLSDYIRQVCEEVGKHLSI"
     gene            3570..3707
                     /locus_tag="ACZPCL_00890"
     CDS             3570..3707
                     /locus_tag="ACZPCL_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161079.1"
                     /translation="MYLDKNGLNVIIKVSRNAKGIEGTSNLMKADREQPVGGKAHVGD
                     R"
     gene            3917..5671
                     /gene="thrS"
                     /locus_tag="ACZPCL_00895"
     CDS             3917..5671
                     /gene="thrS"
                     /locus_tag="ACZPCL_00895"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR1161080.1"
                     /translation="MKIILPDGSVEEAKDELRAIRHTASHVLAQAVKRLYPETKLAIG
                     PAIDDGFYYDFDREGGFTPDDLEKLEAEMKKIVKENLALKPFVLPRDEAIKFMQEKGE
                     PYKVELIEDLPEGETISFYQQGDFVDLCAGPHILYTKGIKAFKLTSIAGAYWRGSEKN
                     KMLTRIYGTAFAKKEDLEAYLTMMEEAKKRDHRKLGKELGLFMFAEEGPGFPFFLPKG
                     MTLKNTLIDYWREIHLREGYQEVSTPVILSRKLWETSGHWDHYKENMYTTVIDDEDYA
                     IKPMNCPGGMLVYKSQPRSYRDLPLRVGELGLVHRHEKSGQLHGLMRVRCFTQDDAHI
                     FMTQDQITDEIKGVTRLINEVYSQFGFDYFVELSTRPEDSMGSDEDWEMATNGLRNAL
                     DEMGLKYIVNEGDGAFYGPKIDFHLRDSIGRTWQCGTIQLDFQLPQRFEAEYVAEDGT
                     KKRPIMIHRVCFGSIERFIGILIEHFAGKFPVWLAPVQVKVIPVSEKSMEYAQGVYDQ
                     LKAAGIRTELDRKDEKVGYKIRQAQLEKVPFMLVLGEKEAAEGKITVRSRDKGDLGVA
                     DLSTFIADVKKMTETREK"
     gene            5847..6413
                     /locus_tag="ACZPCL_00900"
     CDS             5847..6413
                     /locus_tag="ACZPCL_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR1161081.1"
                     /translation="MKIAVIGYTGSGKSVLAAKLGKILGCPVLHLDKLQFEAGWKERK
                     EAEGNRLCEQFLEENKERGWVIDGNYEKFCQKRRMKEADLIIFMDYPRWICLWQALRR
                     YYRYKGHTRKSMADGCIEKMDKEFILWILKNGRSREKKEDYKRVGAQYPDKFIQVRNR
                     RQLRATVIRLLQLQNEKGLYAGTGSRKR"
     gene            6410..7894
                     /locus_tag="ACZPCL_00905"
     CDS             6410..7894
                     /locus_tag="ACZPCL_00905"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1161082.1"
                     /translation="MKKEELLIERLKKYRRSDMYPFHMPGHKRAEGIKLSFPDPFSVD
                     ITEIDGFDNLHHPEGILKESMKWASSLYGSDHTWYLVNGSTCGLLSAISAAVPHRGKI
                     LVSRNCHKAVYHGIYLNHLEAVYVYPQSVPGLGIQGGILPEDVENALKKDPDIQAVLM
                     VSPTYDGIVSDVKAIAEIVHKAGLPLIVDEAHGAHFAYGDAFPKSALELGADAVIQSV
                     HKTLPSLTQTALLHVKNNRPDGGCYLDVKKVERYLQIYQSSSPSYVLMASIENSIFLM
                     DQYRKEGKVAEFEAALLKIRKKLGKMKPLRLVERDLRGTAGIFDVDISKIVVSVRGSG
                     LTGEMLSKILREKYHLEMEMCGADYVTAIAAIGDSKKGLKRLKKALLEIDKELEKNGN
                     ICNDDPDENVYSRHAKQVMPVFKAMDGEKEAVPLKESCGRICGEFAYIYPPGIPLLAP
                     GEQITEEILKTLQDYIEKNLPVQGLEDTDLVTIQVMALSGGRSV"
     gene            7920..8489
                     /locus_tag="ACZPCL_00910"
                     /pseudo
     CDS             7920..8489
                     /locus_tag="ACZPCL_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584582.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
     gene            8517..9383
                     /locus_tag="ACZPCL_00915"
     CDS             8517..9383
                     /locus_tag="ACZPCL_00915"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1161083.1"
                     /translation="MLKKIGFIGVGIMGKSMVRNLMKAGYEVSIYTRTKSKVEDVIAE
                     GAVWCDTVADCSKGRDVVITIVGYPKDVEEVYFGENGILENADKGTYVIDMTTTSPKL
                     DQQIYEEAKKRGLHGLDAPVTGGDSGAKAGTLTILVGGDKEDFDTCLPVFEAMGKAIN
                     YEGKSGNGQHTKMCNQIAIAGALAGACEAMVYAKNAGLDVDVMLKSISTGAAGSAQMN
                     NVASKAAKDDYAPGFFLKHFIKDMSLADEEASERGTKLDVLEDVLGICKKLEEEGMGD
                     LGTQALIKHYKW"
     gene            9818..10945
                     /locus_tag="ACZPCL_00920"
     CDS             9818..10945
                     /locus_tag="ACZPCL_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1161084.1"
                     /translation="MKKRFAAASLAAMMVLSMTACGGGNTATTAAPAETKAEETTAAE
                     AKEESKEEAKESEATAEKAPEDYSGTVVVYSPHDADPLNAGVNLFMEKYPNVKVEVVA
                     AGTGELCNRIAAESANPIADVLWGGGADSLAAFKDYFAPYVCANDDVIAEEYKDPDDK
                     WIGESPLPMVLFYNKDLIEKDGMTIPETWEDLTKPEWKGKIAYCLPSKSGSAYTQLCT
                     MILGHGGKEDGWDFIKKLYDNLDGKIVDSSGKCHKMVADGEFYVGLTLEKAAVQYKDD
                     PSVGFVYPKDGTSAVPDGVALVKGCPNEENAKLFIDFVTSKECQTEQSENWGRRPVRS
                     DMDVADGLAKLSDLVLVDYDFDWAANEKESIIEHFNDIMVD"
     gene            11115..12194
                     /locus_tag="ACZPCL_00925"
     CDS             11115..12194
                     /locus_tag="ACZPCL_00925"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1161085.1"
                     /translation="MSHAVIIKNAVKKYGDFTALNGVSLDIKQGEFFTLLGPSGCGKT
                     TLLRMIAGFNTVDGGEICFDDRVINNLEAHKRDIGMVFQNYAIFPHLTVAENVAYGLK
                     AKKCPKDQIPGKVEEALELVQIGKLKDRKPNELSGGQQQRVALARAFVIEPGVLLMDE
                     PLSNLDAKLRVQMRTVIKKLQRRLGITTIYVTHDQEEALAISDRIAVMKEGNIMQVGT
                     PEEIYKKPENTFVAGFIGVSNFVECDVKGDDPNQAVLDIKGECSITCTLKKPFTGKGI
                     ISARPEQLFFDDKEGLPGTIIISTFLGDFIEYEILLDNGQTVQLNEYTKDVHELRPDG
                     QKVKVNFDIRAVGVYDAATQEVISW"
     gene            12188..13840
                     /locus_tag="ACZPCL_00930"
     CDS             12188..13840
                     /locus_tag="ACZPCL_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161086.1"
                     /translation="MVGSGKKLDFWFWVKVAVVGFMLVFLIYPFCTLITRSFFSTKVD
                     GFTFENYIRFFTKKYYYSALGRSIFVSVVTTITTLVVGVPIAYVMSRYNVWGKQFIHI
                     FIIMSLMSPPFIGAYSWIMLFGRSGLVTNMFSQFGIHLPSIYGKLGIILVFTFKLFPY
                     VYLYTSGAMGSIDSSLEEAAENLGSNKLRRLWTITLPVVLPSIAAGAIMVFMTSLADF
                     GTPMLIGEGYMVLPVLVYNEYMSEIGGNAHLASALSVVVVFCSTTVLLVQKHFVTKKN
                     YVMTAMRPPKEEELHGVKRFLATFPVMLVTFIGILPQIVVIVSSFIKSDFTGFKKGFS
                     LGSYITIFNRLWTNIRNTFVFSTIAIIFIIIIGMLISYIVVRQKGFAGQLMDLLIMFP
                     FVIPGAVLGISLIVAFNKEPVILTGTAVIMIIAFVVRKLPYTVRSGSAFLQQMDPSVE
                     EASISLGVSPMQTFIKVTARLMAPGVLSGAILSWITCINELSSSVMLYGGKTSTISVA
                     IYTEVVRNSFGTAAALASILTVSTVISLLIFLKVSKGKVSIV"
     gene            13934..15373
                     /locus_tag="ACZPCL_00935"
     CDS             13934..15373
                     /locus_tag="ACZPCL_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HIR1161087.1"
                     /translation="MVKFGTGGWRAIIGEEFTKENIQKLTLAMCLKMKAEHKDTKSVV
                     IGYDRRFLSKEAVIWACQILGKEGIHVQFVNRSAPTPLLMFYVMKHELDYGMMVTASH
                     NPAIYNGIKVFTYGGRDADEIQTKDIEKWLLEAEKLYTPCHEEDGQMPPPSYTELVKQ
                     GIVEEINPMNEYLDNILSVINVDAIRKRDLRVAVDPMYGVSLTALSTILAVARCTVET
                     INAQHDTLFGGKMPAPAEDTLKNLQNYVLDRYCDIGIATDGDADRLGVIDDKGHYLHA
                     NQILVMLYYYLLKYKGWRGDAVRNLATTHMLDKVAESFGQKCYEVPVGFKYISAKMQE
                     TDAIIGGESSGGLTVKGHIHGKDGIYAASLLVEMIAVTGKSLSEIVKDIEDQYGETHM
                     EERSYRFTAEEKARIQKVLLEEQKLPELPFEVSRVSYQDGCKVYFKNGGWVSARFSGT
                     EPLLRIFCEMEKEEDAVRACVVFERFLGL"
     gene            15614..16543
                     /locus_tag="ACZPCL_00940"
     CDS             15614..16543
                     /locus_tag="ACZPCL_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024921.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR1161088.1"
                     /translation="MAGTVLTGCSRADDLVQEQTETENYALTVFTAQERKEWEPVIKE
                     VEERTGWNVRVETGSAKEMLSQLEKEEADWDVAFGVSADALEEGKEYWQTGKDAWTGY
                     SAVSFVILYNTNVVTYRELPTGWDSLLEPRWKGRVAVPDPLKSDMAASVLTEAAKNSE
                     KEEFLDLLAENMQYQMPETFKEVEQGISDGRYSIGVTSEEAAKAMLAAGQDVDYIDPE
                     GGECFFEDGTAIRKGTPNLDASEAFVTYTTCDDTKWLLRTQLLRQPAEDVAAENDRSD
                     EELKQRYSRQQEVLNIWKLIMEEKTGPAHEQEQ"
     gene            16564..18294
                     /locus_tag="ACZPCL_00945"
     CDS             16564..18294
                     /locus_tag="ACZPCL_00945"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing sensor histidine kinase"
                     /protein_id="HIR1161089.1"
                     /translation="MKILNKLSFHKKIFLACLLTALVPLLCSSVVMIRLFTAALDRQN
                     LDEGRTQITKAEARLEAVFEKCEETCKTIATDKKTARIMIEKSEADHQREMYLSLYQA
                     TQETSGYARFSIYDSGGRLCYTTDTEGKEKDLPVFWGLLRKASRTDDIVYYRTDPDLS
                     ITDGDILLQGARPLYTEGGAKTGYIVFDFTRENLDDLLGAEVSSGDVLLLLDTHKRTV
                     YCSGQDKSQVQTGDMIEKILENRKMSDAGHDREQYLVSHGGKTGFYFLLCRQAPMSQP
                     AVQTMWTVSLCLSALGLFLCLAVSGVLTGSIARPVQTLDEAMEKVKKGDLSIRIHTQS
                     NDEMGRLTRSFNQMTEDLGRYLEDKVQRQKDLNETTLKLYQTQLNPHFLYNTLDTIKW
                     EARIRQVPRIAVLAENLAIILRKSISSKPFIPLREELETIDSYVEVQKIRFTGRFLCE
                     KEIPDQLEECLVPKMILQPLVENAIIHGLEGCENGYICIYAGREGDVLNISITDDGRG
                     MSPEILAWINSPEPEKRDGHLGLYNIMRILKLYYGNEYGLKAEVTEDGTTVTLRLPDV
                     RSGSFGAERP"
     gene            18322..19092
                     /locus_tag="ACZPCL_00950"
     CDS             18322..19092
                     /locus_tag="ACZPCL_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1161090.1"
                     /translation="MYKVVVVEDEEIARKGIIFTINWDALNCMIAGEAANGEEGAEVI
                     RRLSPDIIVTDLKMPRMDGVEMIAKLREQGNRAKFIILTAYGDFKYAQSAVKLGVSDY
                     LLKPLKDGDLEQAVTRIIDQLEEGDRLRQKEEEETPIFRFNADRKAKNKYVEQAIKQI
                     REHYKEDINISTVAEQLQISEGYLSRVFKKETDYTFTTYLSYYRMKVAMELLKEGNLK
                     VYEVADAAGYSDTAYFSAQFKKIVGIAPSEYQDRVRGH"
     gene            19256..20245
                     /gene="holB"
                     /locus_tag="ACZPCL_00955"
     CDS             19256..20245
                     /gene="holB"
                     /locus_tag="ACZPCL_00955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIR1161091.1"
                     /translation="MLEFKDILGHEQIKEHFQNAAATGKVSHAYILSGEEGMGKKTLA
                     NAFAMTLLCEEGGKEPCMQCHACKQVLSGNHPDLIYVTHEKPASMGVDDIREQINDTI
                     MVRPYSSQYKIYIVDEAQKMTVQAQNALLKTIEEPPAYAVIMLLTTNQDAFLQTILSR
                     CVQLKLKPLKDSVVKTYLEEKLQVKDIQAEIYSAFARGNLGKAIHLAQSEDFKMMYEE
                     ILKLLKEIKEMDISQLLDVIRKLKDDNTDIKECLDFMQMWYRDILMYKTTKDLNLLIF
                     KDEFSAVKKAASLSSYEGLEQILGAIDKARVRLDANVNMELVMELLLLTMKEN"
     gene            20295..21212
                     /locus_tag="ACZPCL_00960"
     CDS             20295..21212
                     /locus_tag="ACZPCL_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIR1161092.1"
                     /translation="MLTVIGVRFRAAGKIYYFDPADRQIKIGDHVIVETARGIEYGYV
                     VLGNREVDETKVIPPLKPVIRMATDEDRAIEAKNKEKEKEAFKICQEKIKKHNLEMKL
                     IDAEYTFDNNKVLFYFTADGRIDFRELVKDLASVFKTRIELRQVGVRDETKIMGGIGI
                     CGRPLCCHSYLSEFIPVSIKMAKEQNLSLNPTKISGVCGRLMCCLKNEEETYEVLNSK
                     LPGIGDTVTTADGLRGEVHSVNVLRQTVKVIVVVDKDEKEIREYKVDQLKFKPRRKKG
                     KGGEKQDEAELKKLEALEKREGKSRLNDK"
     gene            21217..21978
                     /locus_tag="ACZPCL_00965"
     CDS             21217..21978
                     /locus_tag="ACZPCL_00965"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIR1161093.1"
                     /translation="MEHSWRQEGNILLRDDERIDDLQRNHYGIIQRKGTFCFGMDAVL
                     LSGFAVVKPGEKALDLGTGTGIIPILLEAKTEGRHFTGLEIQEESADMARRSVKYNHL
                     EEKVEIVTGDIKEAGSRFALASFDVVTSNPPYMNDAHGIKNPGDAKAIARHEVLCTLD
                     DVVREGTRVLKPGGRFYMVHRPRRLIEILQTMKQHGLEPKRMKFVHPYKDREANMVLI
                     EAVRGGGALMKVEAPVIVFDENGEYSQEIRTTYGY"
     gene            22003..22848
                     /gene="rsmI"
                     /locus_tag="ACZPCL_00970"
     CDS             22003..22848
                     /gene="rsmI"
                     /locus_tag="ACZPCL_00970"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR1161094.1"
                     /translation="MTGKLYLCATPIGNLEDITYRVLKTLKEADLIAAEDTRHSIKLL
                     NHFDIKTPMTSYHEYNKVEKAKYLVEKMAEGTNIALITDAGTPGISDPGEELVRQCYE
                     AGIPVTSLPGPAACITALTMSGLPTRRFCFEAFLPAEKSDKKERQRILEELKKETRTM
                     IVYEAPHHLVATLEDLYGALGNRKITICRELTKKFESAFQTTFEEALAHYETEEPKGE
                     CVIVIAGKPVEEIREEEIRAWEEMPIEEHMEHYLSQGMDKKEAMKAVAKDRGISKRDV
                     YQQLL"
     gene            complement(23007..23270)
                     /locus_tag="ACZPCL_00975"
     CDS             complement(23007..23270)
                     /locus_tag="ACZPCL_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR1161095.1"
                     /translation="MKTTGVIRKLDELGRITLPIELRRSLNLDVKDALEITVQDDCII
                     LKKAANTDIFTGGTEDLVEYEGKKVSKASILALAKLAGLKLAE"
     gene            23552..24514
                     /locus_tag="ACZPCL_00980"
     CDS             23552..24514
                     /locus_tag="ACZPCL_00980"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR1161096.1"
                     /translation="MTTKIIGTGSYVPAQIVTNDDLSKIVETNDQWIRSRTGIGERRI
                     ATDDSTSDMAAKAAARAIEQAGIDPEEIDLIILATSSPDYCFPNGACEVQAKVGAVNA
                     ACFDISSACTGFVFALSTAHAYISSGLYKTALVIGADLLSKLVDWTDRSTCVLFGDGA
                     GAAVVTAAENGIIGLNMHSNGAKGGVLTCGSRSTGNFLLGKKPELGYMTMEGQEVFKF
                     AVKQVPECIKEVLEETGNTAEDIDQFIIHQANYRIIESIAKRLKVDLEKFPVNMEHYG
                     NTSGASVPILLDELNRAGKLKNGDKLVLAGFGAGLTWGAVLLEW"
     gene            24616..24849
                     /gene="acpP"
                     /locus_tag="ACZPCL_00985"
     CDS             24616..24849
                     /gene="acpP"
                     /locus_tag="ACZPCL_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1161097.1"
                     /translation="MLEKMKELIADQLSVDADSITEASSFKDDLGADSLDLFELVMAL
                     EDEYSVEIPAEDLENLATVGDVMNYLKNKGVEA"
     gene            25008..25934
                     /gene="fabK"
                     /locus_tag="ACZPCL_00990"
     CDS             25008..25934
                     /gene="fabK"
                     /locus_tag="ACZPCL_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HIR1161098.1"
                     /translation="MNTRITEMLGIEYPIIQGGMAWVAEHNLAAAVSEAGGLGLIGGA
                     NAPGEVVREEIRKARELTKKPFGVNVMLLSPHADDVAKVVVEEGIKVVTTGAGNPEKY
                     MDMWKAAGVKVIPVVASVALARRMEKYGADAVVAEGMESGGHIGEETTMTLVPQVADA
                     VSIPVIAAGGIGDGRGIAAAFMLGAEAVQIGTRFVVSKESIVHENYKERIIKAKDIDS
                     TVTGRTHGHPVRCLRNQMTREYIKLEQEGKSFEELEYLTLGTLRKAVQEGDVKNGTVM
                     AGQIAGLISKEQTCKEMIEEMMGQAEKLLGRC"
     gene            25973..26899
                     /gene="fabD"
                     /locus_tag="ACZPCL_00995"
     CDS             25973..26899
                     /gene="fabD"
                     /locus_tag="ACZPCL_00995"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR1161099.1"
                     /translation="MSKIAFIFPGQGAQTCGMGKDFYEQTETGRKVFDKATELLGFSV
                     PELCFEPNDRLDITEYTQAAMVTTSIAMMKVLEENGIKPDVAAGLSLGEYCALAAAGV
                     MSEEDAITTVRQRGIFMQEEVPAGEGAMAAILALDAAVIEEVTADIDGVWIANYNCPG
                     QIVISGKKAAVEEACEKLKAAGAKRAIMLNVSGPFHSGMLVGAGEKLGKVLENVEIHK
                     PVIPYAANVTAQYVTEAEPVKDLLVKQVSSSVKWQQCVETMLADGVDTFIEIGPGKTL
                     AGFMRKIDRSVKTLNVEKLEDIEKVVEALKEA"
     gene            26913..27653
                     /gene="fabG"
                     /locus_tag="ACZPCL_01000"
     CDS             26913..27653
                     /gene="fabG"
                     /locus_tag="ACZPCL_01000"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR1161100.1"
                     /translation="MLEGKIALVTGASRGIGRQIAKTLAAKGAFVIVNYNGSAAKAEE
                     VVKEIQAAGGNGQAVQCNVSDFESCKEMLDAVVKEHGRLDILVNNAGITRDNLLMKMS
                     EEDFDAVIQTNLKGVFNCTRHIARQMLKQKSGRIINISSVSGVLGNAGQANYCAAKAG
                     VIGLTKSAAKELASRGITVNAVAPGFIKTEMTDVLKDDIKKAIMENIPMKAFGETEDI
                     ANAVAFLASEEARYITGQVISVDGGMAM"
     gene            27718..28956
                     /gene="fabF"
                     /locus_tag="ACZPCL_01005"
     CDS             27718..28956
                     /gene="fabF"
                     /locus_tag="ACZPCL_01005"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR1161101.1"
                     /translation="MNTRVVVTGMGAITPIGNSVEAFWTAVKSNTVGIAPITHFDTAD
                     FKCKLAAEVKDFDPKQYMDPKTARRMETFCQFAVAAAKEALETSGIDMEKEDPFRVGV
                     SVGSGIGSLQALERNEKRLLEKGPGRVEPLLVPLMISNMAAGNVSIQFGLKGKCINVV
                     TACATGTHSIGEAFRSIQHGEADVMVAGGTEASITPIGVAGFTSLTALNTTDDVKKAS
                     IPFDQDRNGFVMGEGAGIVVLESLEHAQARGAKILAEVVGYGATCDAYHITSPAEDGS
                     GAAKAMEFAMKDAGITPEQLDYVNAHGTSTHHNDLFETKAIKLALGDHANKVKINSTK
                     SMIGHLLGAAGGVEFITCVKSIEDGYVHPTVGLENPGEGCDLDYTMHEGVSMDVNYAI
                     SNSLGFGGHNASLIVKKFTA"
     gene            28974..29471
                     /gene="accB"
                     /locus_tag="ACZPCL_01010"
     CDS             28974..29471
                     /gene="accB"
                     /locus_tag="ACZPCL_01010"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR1161102.1"
                     /translation="MNMDEILTLVQAVSDSKLTSFKLDQGDMHISLKKEKEEAKIITV
                     GAPGAVAAVPAPSAAPVVTAASAGVSTEAPAAAPEAAESHDIASDKVITSPLVGTFYS
                     SSSPDAESFVKEGDTVKKGQVLGIIEAMKLMNEIESEYDGVIEAILVNNEEVVEYGQP
                     LFRIK"
     gene            29552..29977
                     /gene="fabZ"
                     /locus_tag="ACZPCL_01015"
     CDS             29552..29977
                     /gene="fabZ"
                     /locus_tag="ACZPCL_01015"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR1161103.1"
                     /translation="MLMGIKEIQEIIPHRHPFLLVDCIEELEPGVKAVGYKSVTYDEP
                     YFRGHFPQEPVMPGVLIVEALAQTGAVAILSQENFKGKTAYFGGLNNVKFKHKVVPGD
                     RLRLECEIIKQKGPVGVGKATATVDGKIAVVGELTFMIG"
     gene            30005..31357
                     /locus_tag="ACZPCL_01020"
     CDS             30005..31357
                     /locus_tag="ACZPCL_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR1161104.1"
                     /translation="MFNKILIANRGEIAVRIIRACREMGIRTVAVYSEADKDCLHTLL
                     ADEAICIGPAPSSQSYLNMERILSATVAMKADAIHPGFGFLSENARFAKLCQQCNITF
                     IGPSAEIINRMGNKSEARNTMMQAGVPVVPGSKEPVYTAEDGLAMAKEIGFPVMIKAS
                     SGGGGKGMRISRSEEDFTEHFNAAQLESVKGFSDDTMYIEKYIEKPRHVEFQIMGDKF
                     GHVVHLGERDCSIQRRHQKVMEESPCEVISPELRKKMGEVAVKAAKAVNYENAGTIEF
                     LLDKDKNFYFMEMNTRIQVEHPVTEMVSGIDLIKEQIRVAAGEPLSVSQEDIQIKGHA
                     IECRINAENPKKHFMPCPGRITNVHIPGGNGVRMDTHIYNDYKVPANYDSMLMKLIVY
                     DKDRASAIAKMRSALGEVIIEGIETNIDFQYEILENEAFQKGDTDTGFIEKYFPDYIK
                     "
     gene            31445..33169
                     /locus_tag="ACZPCL_01025"
     CDS             31445..33169
                     /locus_tag="ACZPCL_01025"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR1161105.1"
                     /translation="MLRNMFRKTYTKIDTKYRPVSHEGEEPSIPEGLWRKCNKCGQPI
                     YVEDVKNNYYVCPKCGGYFRVHAYRRIEMLTDVGTFEEWNKEMPFSNPLDFPNYEKKV
                     EAAREKTNLNEAIVIGKAKIDGNPAVVGVCDARFIMSSMGHVVGEKITEAVERATKEK
                     LPVILFACSGGARMQEGIVSLMQMAKTSAALKRHHEAGQLFISVLTDPTTGGVTASFA
                     MLGDIIIAEPGALIGFAGPRVIEQTIGQKLPEGFQRAEFLLEHGFVDMILPRQDQKHV
                     IGRILYMHRKHDMDVEENGVKTDIPVNTLAAAQSQENTEKSAWDTVLLSRKADRPTAL
                     DYITAVFDEFMEFHGDRCFKDDGAIVGGIAMFHGMPVTVIGQQKGKNTKDNIRRNFGM
                     PSPDGYRKALRLMKQAETFGRPIICFVDTPGAFCGLEAEERGQGEAIARNLFEMSDLK
                     VPVLSIVIGEGGSGGALAMAVANEVWMMENAIYSILSPEGFASILYKDSKKAPEAARV
                     MKVTAADLKELGLIERIIPEEEPANTDTLYRIAGYMDRSMVKFMKKYQDMSGEELAEH
                     RYERFRRM"
     gene            33169..34341
                     /locus_tag="ACZPCL_01030"
     CDS             33169..34341
                     /locus_tag="ACZPCL_01030"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR1161106.1"
                     /translation="MSGIKALKIGDLVLQRPVIQGGMGVGISLHKLAGAVAASGGMGL
                     ISTAQIGFREPDFKTNFVEANLRAIRREMQKAREIAPKGAIGFNIMVATKHYDLWVKE
                     AIKSGADAIVSGAGLPVRLPEYAQAAYEEMKAGIADAKENCEEFCKQAVKKVKLAPIV
                     SSAKSAQVICRLWDRKYKQVPDFVVVEGPLAGGHLGFSREELAEYGADTKDVPNTYNQ
                     EAYDKEVRSIMEVVKTYEEKYQKHIPVVTAGGIYTHEDVKHQFELGAEGVQVATRFVT
                     TEECDAPDAYKQAYINAKKEDIVITQSPVGMPGRAILNPFLQQIKDGVRPAIKTCFQC
                     LEHCDIKTIPYCITKALVNAAEGDEDNALLFCGSNAYRAEKIEKVDDVMKELVGEL"
     gene            34582..35034
                     /locus_tag="ACZPCL_01035"
     CDS             34582..35034
                     /locus_tag="ACZPCL_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161107.1"
                     /translation="MKKRFAITVLALALTAGSAMTSFAAGFTGTGKGVKYQWGDGAYC
                     TNNWVQYKNHWFYFGDDQLMRTGWIQKDGTWYYAADTGELQGGIMKINGNVYYFDTST
                     CKMVTGNYSYNGGTHEFTENGTTDGGPYVYNEWNSNGIIKRGTKFGVR"
     gene            35180..36001
                     /locus_tag="ACZPCL_01040"
     CDS             35180..36001
                     /locus_tag="ACZPCL_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024803.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1161108.1"
                     /translation="MAENRTEIKQREYLIGDMSEIVGVSRDTLRFYEKKGIIKARKKE
                     NGYRYYLEEDLFRLMVILYKRKMNDSLEEIEGYIKGENSVEYMKKHLQTRIKQEEMKI
                     HYHQQAIARMRLATKDIQMAEQYGGKCTWKNFPVAYQLGVCDSLHASLQKWFSLSSET
                     GGLDMTYFYTEFSIKEGQMQEQGTKLFLYQEVGPFISSDFHLEQYPQTAPVPCIYTIL
                     ESENIYPDEETACQMVRWGKEQGAEPTGVLYSNNMTSFLGEDSNIYFLELYMPVK"
     gene            36353..36982
                     /locus_tag="ACZPCL_01045"
     CDS             36353..36982
                     /locus_tag="ACZPCL_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HIR1161109.1"
                     /translation="MLNYIWSGMLFLGIIWGTFHGRLAQVTEGALNAAGEAVTLSITM
                     LGIVVFWNGILEIGSRAGLVEQLSEKMRPALKFLFPDLPPDCEAARQICVNFIANILG
                     LGWAATPAGLKAMKELEKVEEERRKEKVAKRTIHAVSKGTASNEMCTFLIMNISSLQL
                     IPVNIIAYRSQYHSTSPAAVTGPCFAATMVSTLAAVIFCKIMDHLRNPT"
     gene            complement(37044..38384)
                     /locus_tag="ACZPCL_01050"
     CDS             complement(37044..38384)
                     /locus_tag="ACZPCL_01050"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR1161110.1"
                     /translation="MSKVWEFFENMNEAVYASDVDTYELLYLNKAARDLFQFKDESDY
                     KGKNCYEILQQCSSPCAMCTNNRLKPGVFYEWSRFNPLVRRSYLLKDTMVIDDGRRVR
                     IEMAIDLDIHELAKRSFSEFTDNEALINEGLRCALAEETPEQSIDTLLQYLGQMFQSD
                     RVYIFEKNKHGNFDNTYEWCASKVSPQKNILRNIPPETMGTWIPTFRKGESIIIRNVD
                     EIVSYDPTVYETLIPQNIARLIVSPIYRDREVIGFYGIDNPPLDRMDHIAFMLQLLGH
                     FINSMLRRRDLVGKLETLSYHDQLTGAKNRHAMNEKLASLANGGSLGIIYSDVMGLKQ
                     INDDFGHQAGDEALIYACENLKAHFPDKCVYRIGGDEFLVLIPGITENLFQSMIRSML
                     VDMAKSSVHLALGSVWVDDCTGKVEKSLSEADARMYEDKRAYYAKNRQLDRRRH"
     gene            38563..>39246
                     /locus_tag="ACZPCL_01055"
     CDS             38563..>39246
                     /locus_tag="ACZPCL_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HIR1161111.1"
                     /translation="MRQNGNKIWHIKNKRSLKLAAAMLAVSLAFPMAAQAKAWDFENG
                     SYVDASGTPIEGAVAKGITVTKYQNRANRENGIDWGKVAADGIGFAMIRIGYYKDKDP
                     YFDRNITEAAAHGIKTGVFFYTQALDAQTAADEAEYVLQVIKDFPVSYPVAYDVESNY
                     LLEQGLSSAQITENVNAFCKVIADAGYCPIVYANNDWLTNHLNTAEIPYDIWYARYGT
                     INSYPNRTIW"
BASE COUNT        12110 a         7559 c        10049 g         9528 t
ORIGIN      
        1 gaagacgacg aggtagatag gtcagaggtg gaagtgtggt aacacatgga gctgactgat
       61 actaatcggt cgagggcttg accaataagg tttagggaaa aacaacaaag gttgtaagta
      121 ttcggaagat caacagttca atatgtggtt ttgaaggtat gtaaatgcct ttaatggccc
      181 ggtggctcag ctggttagag cgccgccctg tcacggcgga ggtcgacggt tcgatcccgt
      241 tccgggtcgt ttctttttca atttgataaa gaatacagtt ggggtcttag ctcagctggg
      301 agagcatctg ccttacaagc agagggtcac aggttcgagc cctgtaggcc ccattctaag
      361 tataatttat cagaaaaaga gtataatggt tatatgggcg aattcccgag tggccaaagg
      421 ggacagactg taaatctgct ggcactgcct tcggtggttc gaatccacct tcgcccagtt
      481 aaatacttat tgacaaatga aacgtaagta tttataataa tttaggtaac gcggggtgga
      541 gcagtctgga agctcgtcgg gctcataacc cgaaggtcgt aggttcaaat cctgcccccg
      601 caatttttat gccttaggca taaggaaaaa gcccagatag ctcagttggt agagcagagg
      661 actgaaaatc ctcgtgtcgt tggttcgatt ccgactttgg gcatttaagt aatttaataa
      721 gggcgtgtag ctcagtcggt agagcacttg acttttaatc aagttgtccg gggttcgaat
      781 ccccgcacgc tcatctttga aaagtggcgg aaaatcttag aaattgagat tttccgcttt
      841 tttcgtttta tttattttat catatcacga agttattatg aataagagga tggaatgaga
      901 tgagaagaag aaagaaggac tctggtttaa aaacagcgct gttgctgatc gtggccatta
      961 tttttatgat catggccatc agtgttatag tgatcgtttt tggaaagatc cattcagatt
     1021 atgaaaaact ggatatgacc agggcaagtg aaaaacagac actggaaata tcggcagctg
     1081 agacaaaaga aacggatcag ctgggctggg aggaaacaga ggacggctgg aagtataaga
     1141 tcgatgaaaa gacctatgcc gtggatcagt ggctggaaat aaaaggattt ttgtatcatt
     1201 ttgatgacaa ggggatcatg gcaactggtc agtggaaagg agatggccag atctttacct
     1261 gccatgatgt aaaaggatac ctgaaaaata ttgagccgga tcccaactat gtaccggaag
     1321 atacagggga agacatggac agttttatca ggaccaatgc tttctggtgc tatctggaca
     1381 gtgaagatac aggacttttt aagaccattc tgtataagaa aacggtagac aataaggtaa
     1441 aaccacttgg caatgaaaag aacccggaga aagccacgaa aaactcttta agagcttatg
     1501 gagactatgt gtattatctc ccaaaggtgg cagaaagcaa acgatcttct ctttcagaag
     1561 aggagaagga acggtgtgat gtactggtac gtatgatacc gggacagaat acaaaagaga
     1621 tcattgctga aaatgtagac ggatatcttg tattggatga aactatttac tatgcccagg
     1681 aggggaagat ccatacagcc acttccggta cggagacaga actttctgaa ggtggatatc
     1741 aggtaaagat tgacggagat gcatgttatc ttgtaaatgc acttggaaag ccggtaacat
     1801 ctgctggaag cgcaggtatt accgtagaag atcgtgtata caaggttgat gaaaatggta
     1861 agatcagtta tgtaaagaaa aatccggttg ctgcagatga cagttcttac gaacttcagg
     1921 gaacaggatc acagatgcag cttgtacgac ggagttcttc tggaagcaaa actattgcaa
     1981 gagcagatta tggtgtacaa agttactgca ttgtggataa ttccattttt ttcagcgctt
     2041 atgtggaaaa gactgcagat ggaaaatggt acagccgtat ttataaaacg gatctgaatg
     2101 gcaaagagaa aaaggtagtc agcggtattt ttccaggagc tgtttttaat ctctactatt
     2161 ttgaaagcca gggagaaata tatggagagt atcatccgaa gatctgggaa aatgcgtatg
     2221 gtgtagtggt caatgtaaag caggatggta ctatttatgc agtaaaagac acttcggaaa
     2281 gaacgggaaa gaaagttaca ggaaatgata tgatcgagat cctggcactt caggatggga
     2341 agatgatcgg ttattggcat gactgcaact ggagcagaac tggtgggatc acaagtgtac
     2401 tttggagttt accggtttcc ctggatgttt cggcaaaaac gagagttgaa acagtggaag
     2461 agatcaaaga agaggaaaca gggaccagcg tagaagaaac tactacaaca actgcagaaa
     2521 aaatcgttcc tgtaacgccg acacatagtt cggtgtcacc tgtagagaca ggaaaggtgc
     2581 atccgtccgt aaatccaaca gaagttgtga tcggaacaga tgcacctgga aaacgagtag
     2641 aagagactac ggtagcgacc gcgcctattg caccggcgac aacggctgct gaaacaacag
     2701 cagaagaaat taagatcgtg ccgttatcat aaaaagcacc ggaagcaagt ttggagaaaa
     2761 tttaatgaac atcttcctca tcaaaatcaa ctacaacgta aaatccccgt gaattttcac
     2821 ggacatcttt tacaatgcca tattcggatt taatgcggtc tctggcggcg aagcagccgg
     2881 acatagctac agcaggatca ataatgtagc tgtagctgct ggttccttcc cgttcgatga
     2941 tctttacttt gtcaccggcg cttacaaggc cggggcgttc cattttaaat tcaacggttc
     3001 tcatgataag atcaagcctt ctttctttta gatgtttaaa tggataaatg tttgcccact
     3061 tcttcacaga cctggcggat atagtcactt aaactgccgc cctgacagcc tgcgataaaa
     3121 tggttgcagc gatttacggg gataagtatt ttgtaggcgc tgctggagct tacagcggtt
     3181 gccatagcag cggtgatctc ccccatcagg gagtctgcca taacgatacc aataggcccg
     3241 atgatcaggt ctgcgtttct ggcattgacg atcacaggat tttcgccggt ggctccataa
     3301 gaggcaccgg cttttaacat ggcagaggtt gcaatgctgt tggtgccaat ggcgtaaagt
     3361 tcgagttcgg gatagctgct ttttaactgt tcgatcacag cttttcccat tcggccgccc
     3421 tggccatcta tgataacgat cttcataggt tgtcttcctc ctttataatc tcttttatgg
     3481 attacggttg ttgttctcgg aaagtggaga aatgataaat cagagtatat cttatcagaa
     3541 atacagcgga aagggaataa gaggattgat tgtatcttga caaaaatggc ttaaatgtta
     3601 taatcaaggt tagcagaaat gcaaaaggca ttgaaggaac cagtaatctg atgaaagcag
     3661 acagagagca gccggttggt ggaaaggcgc atgtgggaga cagatgaata ccagtcctga
     3721 gccgtatcag ggaacttgaa gaagggaaat gataccgtac cccgcgttaa gggatgaatg
     3781 agtgaaaatc aaggtggaac cgtgtatatg cacccttgga catagagatg tgtccagggg
     3841 ttttttaata cctgaaaacc ttataggaca tatccaaaag atctaaaaac gcgttttaaa
     3901 gcgaaaggag tttaccatga agattatttt accagacgga agtgttgaag aagcaaaaga
     3961 cgaattaaga gccatccgcc acactgcttc ccatgtactg gcacaggcag taaagagact
     4021 gtatccagag accaagctgg caatcggacc ggctattgat gacggatttt actatgactt
     4081 tgacagagaa ggcggattta ccccggatga tctggaaaag ttagaggctg agatgaagaa
     4141 gatcgttaag gagaacctgg cattaaagcc attcgttctg cctagagacg aagcaattaa
     4201 atttatgcag gaaaagggcg agccttacaa ggtagagctg atcgaagatc ttccagaagg
     4261 cgagacaatc agcttctatc agcagggcga tttcgtagac ctgtgcgcag gaccacatat
     4321 cctgtataca aagggcatca aggcatttaa gctgaccagc attgcaggtg cttactggag
     4381 aggtagcgag aagaacaaga tgctgaccag aatttatggt actgcatttg caaagaaaga
     4441 agacctggaa gcttacttaa ccatgatgga agaggctaag aagcgtgacc acagaaaatt
     4501 aggcaaggaa ttaggactgt tcatgtttgc agaagaggga cctggtttcc cattcttcct
     4561 tccaaagggc atgaccttaa agaataccct gatcgactac tggcgtgaga tccacttaag
     4621 agaaggctat caggaagttt ctactcctgt tatcttaagc cgtaagctgt gggagacctc
     4681 cggtcactgg gatcattata aagagaacat gtatacaacc gttattgatg acgaagacta
     4741 tgctattaag ccaatgaact gtccaggcgg tatgctggta tacaagtccc agcctcgttc
     4801 ctaccgcgac ctgccattaa gagttggtga gttaggtctg gtacatcgtc atgagaagag
     4861 cggacagctt cacggtctga tgcgtgtacg ctgcttcacc caggatgatg cacatatctt
     4921 catgacccag gatcagatca ctgacgagat caagggcgtt acaagactga tcaatgaagt
     4981 ttactctcag tttggatttg attatttcgt agagctttct accagaccag aagattccat
     5041 gggatctgat gaagattggg agatggcaac aaacggcctg agaaatgcac tggatgaaat
     5101 gggcttaaag tacattgtaa atgaaggcga cggcgcattc tacggaccta agatcgactt
     5161 ccacttaaga gactctatcg gacgtacctg gcagtgtggt accattcagc ttgacttcca
     5221 gcttccacag cgttttgagg ctgagtatgt agcagaagat ggtactaaga agcgtcctat
     5281 tatgatccac cgtgtatgct tcggttctat cgagcgtttc atcggtatcc tgatcgagca
     5341 tttcgctggc aagttcccag tatggctggc tcctgttcag gttaaggtta ttcctgtatc
     5401 agaaaagtcc atggaatatg cccagggtgt gtatgatcag ttaaaagcag ctggtatccg
     5461 tacagagtta gaccgtaagg atgagaaggt tggatacaag atccgtcagg ctcagttaga
     5521 gaaagttccg ttcatgctgg tattaggtga gaaggaagca gcagaaggca agatcactgt
     5581 aagaagccgt gacaagggcg accttggtgt tgctgatctt agcacattta tcgcagatgt
     5641 taagaaaatg actgaaacaa gagaaaaata atgttacagt tcagccatga catcttcttg
     5701 tttccatatt catgaaaaca agaactgcaa caaaataata aataagctgt tttaggcagc
     5761 caatcccctg cagagacttt ataacaggta taaggccctt gcaggggatt ttttcaatag
     5821 tgctttaaca ttcggggaag gaaaaaatga aaattgcagt gatcggatat accggctccg
     5881 gtaaatcggt gctggcagca aaactgggca agatccttgg ctgtccggtg ctccatttgg
     5941 acaaacttca gtttgaagca ggctggaagg agcgaaagga agcagaaggg aaccggcttt
     6001 gtgagcagtt tttagaggaa aataaagagc gtggctgggt cattgacgga aattacgaaa
     6061 aattctgtca gaaaaggcgg atgaaagaag cagacctgat catttttatg gattatccaa
     6121 gatggatctg tctgtggcag gcgctacgcc gttattacag atacaaaggc cataccagaa
     6181 aaagcatggc agatggctgt atagaaaaga tggataaaga atttatctta tggatcctga
     6241 aaaatggacg cagccgggaa aagaaggaag attataaacg ggtgggggca cagtatccgg
     6301 ataaattcat ccaggtgaga aaccgccgtc agctgcgtgc tacagtgatc cgccttttgc
     6361 agctgcaaaa tgaaaaaggg ctttacgcgg gaaccgggag cagaaaaagg tgaaaaaaga
     6421 ggaacttctc attgaacgtt taaaaaagta ccggagatcg gatatgtatc cctttcatat
     6481 gccgggacat aagcgggcag aaggtataaa actgtccttt ccagatccat tttctgtgga
     6541 tataacagaa attgatggtt ttgacaatct ccatcatcca gaaggaattt taaaagagtc
     6601 catgaaatgg gcatccagcc tttacggttc ggaccacaca tggtatcttg taaatggaag
     6661 cacctgcggg ctgctgtcgg ccatcagcgc agccgttccc cacaggggaa agatattagt
     6721 cagccggaac tgccataagg cggtgtatca tgggatctat ttaaaccatc tggaagcagt
     6781 ttatgtttat ccacagtcgg ttcctggatt ggggatacag ggcggaattt taccggaaga
     6841 tgtggaaaac gcattaaaaa aggatcctga tatccaggca gtactgatgg tttcgcctac
     6901 gtatgacggg atcgtttctg atgtgaaggc aattgcagaa atcgtccata aagcgggtct
     6961 tcccctgatc gtagatgagg ctcatggcgc tcattttgct tatggagatg cgtttccaaa
     7021 gtcagccctt gaactgggag cagatgcggt gatccagagt gtgcataaaa cactgccaag
     7081 tcttacccag acagcgctgc tccatgtgaa aaataacagg ccggatggtg gctgctatct
     7141 ggatgtgaaa aaggtagagc gttatctgca gatctatcag agcagcagtc cttcttatgt
     7201 gcttatggcg tccattgaga acagcatttt tctcatggat cagtatagaa aagaaggtaa
     7261 agtggctgaa tttgaagctg ctttgcttaa gatacgaaaa aaactgggaa aaatgaagcc
     7321 cctgcgcctg gtagaacggg acctgagggg aacagcaggg atttttgatg tggatatttc
     7381 caagatcgtt gtctcagtaa gaggtagcgg gcttaccgga gaaatgcttt ccaagatctt
     7441 aagggaaaaa tatcatctgg aaatggaaat gtgcggtgct gattatgtaa cagccattgc
     7501 agccattgga gacagcaaaa aggggctgaa acgtctgaaa aaggcattac ttgaaattga
     7561 caaagaactg gaaaaaaatg gaaatatctg caatgatgat cctgatgaaa atgtttacag
     7621 ccgtcatgca aagcaggtga tgcctgtttt taaagcaatg gacggggaaa aggaagcagt
     7681 gcctttaaaa gaaagctgcg gccgcatctg cggggaattt gcctatattt atccgccggg
     7741 tatcccgctg ttagcgccgg gagagcagat cacagaagaa atactgaaaa cattacagga
     7801 ttatatagaa aaaaatttgc cggtccaggg actggaagat acagaccttg tcaccataca
     7861 ggtaatggca ttatcaggtg gcaggtccgt ttaaaaagaa agagaaagat ggaaacataa
     7921 tgggaaagat ttattatgta atgggaaaaa gtgcctcagg taaggacacc atttacaaac
     7981 gtctggtaaa ggaactgcca gaacttggaa cagtccgcat gtacaccacc aggcccatcc
     8041 gtgatgggga gaccaatggg gtggagtaca ttttcaccga tgagaagcag cttcaggcta
     8101 tgaaggatgc aggtaaagtg atcgaatgcc gcacttatga taccatttac gggccgtgga
     8161 gttattttac ggcagatgac gggcagatcg atctgggatc cggcagttat ctgatgatgg
     8221 gaacattgga gtcttatgaa aagctctgca gttattatgg ggcagaggtc atggtccctt
     8281 tatatatcca tgtggaagac ggtgaacgtc tgcagcgggc attaaacaga gaaaaaacgc
     8341 aaaaagtgcc aaagtatgcg gaaatgtgcc gaaggttctt agctgatgaa aaggactttg
     8401 caaaagaacg tctggatcag tgcgggatca gaaaacaata tgaaaatacc gggcttgaac
     8461 cgtgtataga agaaatcata aaggacattt tgtgtaacga aggaaaggag aaacagatgt
     8521 taaagaaaat tggatttatt ggtgttggca ttatgggaaa atccatggtg cgcaatctga
     8581 tgaaggctgg ttatgaggtt tctatttata ccagaacaaa gtcaaaggtg gaggatgtga
     8641 tcgcagaggg cgctgtatgg tgtgatacag tggcagactg ctccaaaggc agagatgtgg
     8701 tcattaccat tgttggatat cctaaggatg tagaggaagt atatttcggg gaaaatggta
     8761 tccttgaaaa tgcagataaa ggcacttatg tgatcgacat gaccactaca agcccgaagt
     8821 tggatcagca gatctatgag gaagcaaaga agcgggggct tcatggctta gatgcccctg
     8881 taaccggcgg tgacagcggt gcaaaggcag gaacactgac aattttagta ggcggcgata
     8941 aagaggactt tgatacctgt ctgcctgtat ttgaggcaat gggaaaggct atcaactacg
     9001 aaggaaagtc cggaaacgga cagcacacca agatgtgtaa tcagatcgcc attgctggtg
     9061 ctttagcagg tgcgtgtgaa gctatggtct atgcaaagaa tgcaggactg gacgtagatg
     9121 taatgcttaa gtctatttct accggtgcag caggaagcgc ccagatgaat aatgtagctt
     9181 ccaaggcagc aaaagatgac tatgcaccgg gctttttctt aaagcatttt atcaaggata
     9241 tgagccttgc agatgaggaa gcatccgaaa gaggaaccaa attagatgtg ttagaggatg
     9301 ttcttggaat ttgtaaaaaa ctggaagagg aaggcatggg agatctggga acccaggcgc
     9361 tgatcaagca ttataagtgg taaaatatgg ttgagctgtc tgtgcagaac aaggtaatat
     9421 aaggtaaaaa tattgtaaca gaaaaaatcg acagatgaaa acaggaccgt tgtaaataga
     9481 cgtagtaata gtaagaagtt agctgcgttt atgacaggac ggtcctgttt tattgctggt
     9541 gtatgtatat tatgcacata taaatataaa atacgacata aaaactatac aaaagttcag
     9601 aataacacaa caaaagttag aataacaaat ggaaaaatgc ataataaaat tatataataa
     9661 caacatcagg agtgaaaagt tacggtacat gctatatgat ctgtaacgaa aactccaaag
     9721 aacgtgagaa gcagttttca gactgcaaaa tttatttaag tcccggtgag accgggaacg
     9781 atgtaccggg agggtacata gaaaaggaga ggtgtatatg aaaaaaagat ttgcagcagc
     9841 gtcactggca gccatgatgg tactgagtat gaccgcatgt ggaggcggaa acaccgctac
     9901 taccgcagca ccggcagaga ccaaggcaga ggaaacaaca gcagcagaag ctaaggaaga
     9961 gtctaaagaa gaagcaaaag agtctgaggc aactgctgag aaagctccgg aagattactc
    10021 cggtaccgta gttgtatatt ctcctcatga tgcagatccg ttaaatgcag gtgtgaacct
    10081 gttcatggag aagtatccaa atgttaaggt agaagtagta gcagccggaa ccggcgagct
    10141 gtgcaaccgt attgcagctg agtctgctaa cccgatcgct gatgtactct ggggcggcgg
    10201 tgctgactcc ctggcagcat ttaaggatta cttcgcacca tatgtttgtg caaatgatga
    10261 tgtaattgca gaagagtaca aagacccgga tgacaagtgg atcggtgaaa gcccactgcc
    10321 aatggtactg ttctacaata aagatctgat cgaaaaagac ggaatgacca ttccggagac
    10381 ctgggaagat ctgacaaagc ctgagtggaa gggcaaaatt gcatactgcc tgccatctaa
    10441 atccggttct gcttacaccc agttatgtac tatgatcctg ggtcatggtg gcaaggaaga
    10501 tggatgggat ttcatcaaga aactgtatga caacctggat ggaaagatcg ttgactcttc
    10561 aggtaagtgc cacaagatgg tagctgacgg tgagttctat gtaggtctta ctcttgagaa
    10621 agcagctgtt cagtacaagg atgatccttc tgttggattt gtatatccaa aggatggaac
    10681 cagtgcagtt ccggatggtg tagctcttgt taagggatgc ccgaatgaag aaaatgcaaa
    10741 actgttcatc gacttcgtta cctctaaaga gtgccagaca gagcagagcg agaactgggg
    10801 acgtcgtcct gtaagaagtg atatggacgt tgcagatgga ctggcaaagc tgagcgacct
    10861 ggtattagtt gattatgact ttgactgggc tgcaaatgaa aaagagtcca ttattgaaca
    10921 cttcaatgat attatggtag attaatcttt ctgaaatgaa agtaacggga aacaaataat
    10981 agaaagactg cctggccctg gcgggcgggc agtctttcgc atatataagg aatgtaaaaa
    11041 gtgggagtgt cttccagaaa agcggttgca gaaagatttt gagaggacat tgaacaaaaa
    11101 ggaggaccag atatatgagc catgcagtga tcattaaaaa tgccgtgaaa aaatacggtg
    11161 attttacagc attaaacgga gtaagcctgg atattaaaca gggagaattt tttacccttt
    11221 tagggccgtc cggatgcgga aaaaccaccc ttctgcgtat gatcgcagga tttaatacag
    11281 tagacggagg agagatctgt tttgatgacc gggtgatcaa caatctggag gctcataaaa
    11341 gagacatcgg tatggtcttc cagaactatg cgattttccc acatctgaca gtggctgaaa
    11401 atgtagcata cggcctgaaa gcgaaaaagt gtccaaagga tcagatccct ggaaaagtag
    11461 aagaggccct ggaattggtg cagatcggca agttaaagga cagaaaacca aatgaacttt
    11521 ccggcggaca gcagcagcga gtcgctttag caagagcttt cgttattgaa cctggtgttc
    11581 tgttgatgga cgaaccgctg tctaacctgg atgcaaagct gcgtgtgcag atgagaacgg
    11641 ttattaaaaa gctgcagcgc agactgggaa ttactacgat ttatgtaacc catgaccagg
    11701 aagaggcact ggctatttcc gaccggatcg ctgttatgaa ggaaggaaat attatgcagg
    11761 tgggaactcc tgaagagatt tataaaaagc cggaaaatac ctttgtagcc ggatttatcg
    11821 gtgtttccaa ctttgtagag tgtgatgtta agggagatga cccaaatcag gcagtgctgg
    11881 atattaaggg agagtgttct attacctgta ccttaaagaa gccatttacc ggtaagggaa
    11941 ttatttccgc aagaccggag cagctgttct ttgacgataa agagggactg ccgggaacca
    12001 ttatcatttc cactttctta ggtgacttca ttgaatatga gatcctgttg gataatggac
    12061 agaccgtaca gttaaatgag tatacaaaag atgtacatga acttcgcccg gatggacaaa
    12121 aggtaaaggt aaactttgac atccgcgccg taggtgtata tgatgcagcg acccaggagg
    12181 tgatctcatg gtaggttccg gaaagaagtt agacttctgg ttctgggtga aagtggcagt
    12241 tgtgggattt atgctggtat ttctgatcta tcccttctgt accctgatca cccgaagctt
    12301 tttctcaacc aaggtagacg gctttacctt tgagaactat atccgatttt tcacaaagaa
    12361 atattattat tccgcattgg gaagaagtat ttttgtatct gtggtaacaa cgatcaccac
    12421 actggttgta ggtgtgccta ttgcctatgt aatgtcccgt tataatgtat ggggcaagca
    12481 gttcatccat atttttatca tcatgagcct tatgagcccg ccatttatcg gcgcctacag
    12541 ctggatcatg ttgtttggac gttctggtct ggtgacaaat atgttttccc agtttggtat
    12601 ccatcttcca agtatttacg gaaaactggg tatcatcctg gtatttactt ttaagctgtt
    12661 cccttatgtt tatctgtata cctccggtgc tatgggaagc atcgactcca gtctggaaga
    12721 ggctgcagag aacctgggaa gtaacaagct tcgcagactg tggaccatta cactgccggt
    12781 agtccttccg tctattgcag ccggtgccat tatggtattt atgaccagtc tggcagactt
    12841 tggtactcca atgctcatcg gcgaaggtta tatggtactt cctgttcttg tatataatga
    12901 gtacatgagt gagatcggcg gaaatgccca cctggcaagt gctctttctg tagtagtagt
    12961 attctgttct actaccgtcc ttctggtgca gaagcatttt gttacaaaga aaaactatgt
    13021 aatgactgcc atgcgtccgc caaaggaaga ggaacttcac ggagtaaagc gtttcctggc
    13081 aacattcccg gttatgctgg ttacatttat cggtatcctt cctcagatcg tagttatcgt
    13141 cagcagcttt atcaagagcg actttacagg atttaagaag ggattcagcc tgggaagcta
    13201 tattacgatt ttcaaccgtc tgtggaccaa tatccgcaat acctttgtat tttcaaccat
    13261 tgccattata tttatcatca ttattggtat gctgatctcc tatatcgttg tgcgccagaa
    13321 gggctttgca ggacagttaa tggatctgct gatcatgttc ccattcgtaa ttccaggcgc
    13381 agttcttggt atcagcctga tcgtggcatt taacaaggaa cctgtaatcc ttacaggaac
    13441 cgcagtgatc atgatcattg cctttgttgt acgaaaactg ccttatacgg tacgttccgg
    13501 cagtgctttc ttacagcaga tggatccaag cgtagaagaa gcgtctatca gtcttggagt
    13561 atcacctatg cagacattta tcaaggtgac agcaagatta atggcacctg gcgtattatc
    13621 cggtgcgatc ttaagctgga tcacctgtat caatgagctg tcttccagtg ttatgcttta
    13681 cggcggcaag accagtacca tttctgtagc tatttataca gaggttgtaa gaaacagctt
    13741 tggtacagcg gcagcgctgg catctatttt gaccgtaagt acagttattt ctctgcttat
    13801 cttcttaaag gtaagtaagg gtaaggtatc gatcgtttaa tttgtttcat actgcaacat
    13861 gaaaacagtg cctgcgcctc tggcgcaggc tttgtaagtc tatgtatatt gaaaagatgg
    13921 gaggaacaca caaatggtaa aatttggaac tggcggctgg cgtgcgatca tcggagaaga
    13981 gtttaccaag gaaaatatac agaagctgac cttagccatg tgcctgaaaa tgaaggcaga
    14041 gcataaggac acaaaaagtg tggtcattgg ttatgaccgc cgtttccttt ccaaagaggc
    14101 tgtgatctgg gcatgtcaga tccttggaaa agaaggtatc catgtacagt ttgtaaaccg
    14161 cagcgcacca acgcctctgt taatgtttta tgtgatgaaa catgaactgg actatggaat
    14221 gatggtcaca gccagccata atccggcgat ctacaatggt atcaaggtgt ttacatatgg
    14281 cggaagggat gcagatgaga tccagacaaa ggacattgaa aaatggctgt tggaagcaga
    14341 aaaactgtat acaccatgtc atgaagagga tgggcagatg ccgccgccaa gttatacaga
    14401 actggtaaaa cagggcattg tagaagaaat caatccaatg aatgaatatc tggataatat
    14461 cttatctgtg atcaatgtag atgcgatcag aaagagagat ttaagggtag cagttgaccc
    14521 catgtatggt gtcagcctga cggctttaag caccattctg gcagttgcaa gatgtacggt
    14581 agagaccatt aatgcccagc atgatacgct gtttggaggc aagatgccgg cacctgcgga
    14641 agataccctt aagaacctgc agaattatgt gctggaccgc tactgcgata ttggtattgc
    14701 gacagacggt gacgcggacc ggttaggtgt tattgacgat aagggacatt atcttcacgc
    14761 caaccagatc ttagttatgc tgtattatta tcttctgaaa tataagggtt ggcggggcga
    14821 tgcagtacga aacctggcta ccacccatat gttggacaag gtggctgaaa gctttggcca
    14881 gaagtgttat gaagtgcctg taggctttaa gtatatttct gcaaagatgc aggagacaga
    14941 tgccattatt ggcggtgagt cttccggtgg cctgactgta aaaggtcata tccatggaaa
    15001 agacggtatc tatgcagcaa gccttctggt agaaatgatc gcagtgaccg gtaagagcct
    15061 ttctgagatc gtaaaggata ttgaggacca gtacggtgag actcatatgg aagagcgcag
    15121 ctaccgtttt acagcagagg aaaaggcacg tatacagaaa gtgctgttag aagagcagaa
    15181 actgccggaa ctgccatttg aggtatcccg tgtttcttat caggacggct gcaaggtcta
    15241 ctttaaaaat ggtggctggg taagcgccag attttccggt acagagccgt tacttcgtat
    15301 cttctgtgaa atggaaaaag aggaagatgc agtccgggcc tgcgtagtgt ttgagaggtt
    15361 tttggggctg taaaagtgtg gaaatggaac tgtttttctg gtatactgat ggaacgaaca
    15421 aaccgtccgc attagcggac agtagagcgc gtcagcgcgt gtttgcgagt ctaaaaaata
    15481 caaggggcac cgcagcatgt atacaggaaa agcagacaga aatgcagtat ataaaacaca
    15541 taaatttttc aacagggaat gcaaaaaagg gcgttccctg ttctttttat cggtcatatc
    15601 tgcagctgca atattggctg gaacagttct tacaggctgc agccgggctg atgatctggt
    15661 gcaggaacag acagaaacgg agaactatgc cctgaccgta tttactgccc aggaaaggaa
    15721 agaatgggag ccggtgatca aggaagttga ggagcgtacc ggctggaatg taagggtgga
    15781 aacaggctct gcaaaggaga tgctttccca gctagaaaaa gaggaagcag actgggatgt
    15841 ggcctttggg gtcagtgcgg acgccttaga ggaaggcaaa gagtactggc agacaggcaa
    15901 agatgcctgg accggatatt cagcggtatc ttttgttatt ttatataata ccaatgtagt
    15961 gacctacaga gagttgccta caggatggga cagcttgtta gaaccacggt ggaaaggccg
    16021 tgtggcagtg ccggatcccc ttaaatcgga tatggctgct tctgttctca cagaggcggc
    16081 aaaaaacagc gaaaaagaag aatttctgga cctcttagcg gaaaatatgc agtatcagat
    16141 gccggagacc tttaaagagg tggaacaggg catttctgac ggccgctatt ctattggcgt
    16201 tacctcagag gaggcagcga aagcgatgct ggctgcaggt caggacgtag attacattga
    16261 tccggaagga ggagaatgtt tctttgaaga cggaactgcg atccggaaag gaaccccaaa
    16321 tctggatgct tctgaagcat ttgtaaccta cactacctgt gatgatacaa aatggctttt
    16381 acggacccag cttttacgcc aaccggcaga agatgtggct gcagagaatg accggtcaga
    16441 tgaagagctg aaacagagat attccagaca gcaggaagtt ttaaatatct ggaagcttat
    16501 tatggaagaa aagactggtc cagcccatga acaggaacag taataaagaa ggcagggaaa
    16561 aagatgaaga tattaaataa gctttcattt cataaaaaga tctttcttgc ctgtctgctg
    16621 acggcactgg tgcctttatt gtgctccagt gtggttatga tccggctgtt tacggcagca
    16681 cttgaccggc agaatttaga tgaggggcgg actcagatca caaaggcaga ggcgcgattg
    16741 gaagcagttt ttgaaaaatg cgaggaaacc tgcaagacca ttgccacgga taaaaagaca
    16801 gcccggatta tgatcgaaaa aagtgaagca gatcaccaga gggagatgta cttatcctta
    16861 tatcaggcca cccaggaaac ctcaggatat gcccggtttt ctatttatga ttcaggaggc
    16921 cgtctgtgct atacgacgga tacagagggg aaggaaaaag acctgcctgt attctggggg
    16981 cttttaagaa aggcttccag aacggatgat attgtatatt acaggactga tccggatctt
    17041 tctattacgg acggggacat tctgcttcag ggtgcaagac cgctgtatac agaaggcggt
    17101 gcaaaaaccg gctatattgt ttttgatttt acaagggaaa acctggatga tcttttaggg
    17161 gcagaggtgt catctgggga tgtgctgctg cttttagata cccataaacg gacggtgtac
    17221 tgctctggac aggacaagag ccaggttcag acaggtgaca tgatcgaaaa gatactggaa
    17281 aacaggaaaa tgtccgatgc aggccatgac agggaacagt atctggtaag ccatggagga
    17341 aaaactggct tttattttct cttatgccgc caggcgccta tgagccagcc agctgtgcag
    17401 actatgtgga ccgtcagcct atgcctgtca gcgttgggat tattcctctg cctggctgta
    17461 tcaggcgtac ttacaggcag tattgcccgc ccggtacaga cattagatga agctatggaa
    17521 aaagtgaaaa agggtgatct ttctatccgt atccataccc agtccaatga tgaaatggga
    17581 cgtcttaccc gcagctttaa ccagatgaca gaagatctgg gccggtatct ggaagataag
    17641 gtgcagaggc agaaggatct gaatgaaaca acattaaaat tataccagac ccagttaaat
    17701 ccccattttc tctataacac gctggacacc atcaaatggg aagcacggat ccgccaggtg
    17761 ccacggatcg cagtgctggc ggaaaatctg gcgatcatat tgcgaaaaag catttccagt
    17821 aagccgttta tcccgttaag agaggaattg gagaccattg acagctatgt agaagtgcag
    17881 aagatccgct ttacaggccg gttcttgtgt gaaaaggaga taccggacca gttagaggag
    17941 tgcctggtgc cgaaaatgat cttacagccg ttggttgaaa atgctattat tcatgggctg
    18001 gaaggctgtg aaaatggata tatctgtatt tatgcaggcc gtgaaggaga tgttttaaat
    18061 atttccatta ccgatgacgg ccgtggcatg agcccggaga tattggcctg gatcaacagt
    18121 ccggaaccgg agaagcggga cggacatctg gggctttata atattatgag gatattaaaa
    18181 ttatattatg gaaatgaata tggcctgaag gcagaagtga cagaagacgg aacaactgtc
    18241 actttgagac tgccggatgt tcgctcggga tcttttggtg ccgaaagacc ctgagaggac
    18301 atcttagaaa ggagggggac gatgtataaa gtagtagtgg tagaagatga agagattgcc
    18361 agaaaaggca ttatatttac cattaactgg gatgcattaa actgtatgat cgccggggaa
    18421 gctgccaatg gggaagaagg ggcagaggtg atccgccgcc tttcaccgga tattatcgtt
    18481 acagacctga aaatgccccg tatggacggc gtggaaatga tcgcaaagct gcgggaacag
    18541 ggaaacaggg cgaaatttat tatcctgacg gcttatgggg attttaaata tgcccagagt
    18601 gcggtgaagc tgggagtcag cgattatctg ttaaagccat taaaggacgg tgatttggag
    18661 caggcagtga cccggatcat cgaccagtta gaagaaggag accggctccg gcagaaagaa
    18721 gaggaagaaa cgcctatttt ccggtttaat gcagacagaa aagcaaaaaa taagtatgta
    18781 gagcaggcga tcaaacagat ccgtgaacat tataaagaag atatcaatat cagtacagtg
    18841 gcagagcagc tgcagatcag cgaaggctat ttaagccgtg tgttcaaaaa agagacagat
    18901 tatacattta ccacctatct ttcttattac cgcatgaaag tggcaatgga actgttaaaa
    18961 gagggaaatt taaaggtata tgaagtggca gatgctgcgg gctattcaga tacagcctat
    19021 ttcagcgccc agtttaagaa aatagtgggg atcgcgcctt ctgagtatca ggatcgggta
    19081 agagggcact aggcggctgg tcatggagcc tgtgaatggt aattgcactg gagatttttg
    19141 cacctggaaa aagtgtgtac aggaaaaagt aattatgtta tcatagtgat aagatcgtga
    19201 gagcacaaag tgcagagcat ccggtaacaa acagggtact gggggaggtg gccacatgct
    19261 tgaatttaaa gacatcttag gacatgaaca gatcaaagaa cattttcaga atgcggcagc
    19321 aacggggaaa gtttcccacg cttatattct aagcggcgaa gaaggaatgg gaaaaaagac
    19381 ccttgcaaat gcctttgcca tgactcttct ctgtgaagaa ggaggcaaag agccctgtat
    19441 gcagtgtcat gcctgcaagc aggtcctgtc aggaaaccac ccggatctga tctatgtaac
    19501 acatgaaaaa ccggccagta tgggtgtaga tgatatccgt gagcagatca atgataccat
    19561 tatggtgcgt ccttacagca gccagtataa gatctatatt gtagatgaag cgcagaaaat
    19621 gacggtgcag gcccagaatg ctcttttaaa gactatagaa gagccaccgg cctatgcagt
    19681 gatcatgctt ttgactacaa accaggatgc atttttacag accattcttt cacggtgtgt
    19741 gcagctgaaa ctgaaaccat taaaagactc tgttgtgaag acttatctgg aagaaaagct
    19801 tcaggtaaag gacattcagg cagaaatata cagtgcattt gcaagaggaa atctgggcaa
    19861 agcaattcat ctggcccagt cagaggactt taagatgatg tatgaagaga ttttaaagct
    19921 gttaaaggaa atcaaggaaa tggatatttc ccagcttttg gatgtgatcc gcaaattaaa
    19981 agatgacaat acggatatta aggaatgtct ggattttatg cagatgtggt accgtgatat
    20041 cctgatgtat aaaactacca aagatttaaa ccttctcatt ttcaaggatg agttttccgc
    20101 agtgaagaag gcggcgtctt taagcagtta tgaagggctg gaacagatct taggggccat
    20161 tgacaaggcc agagtccgtt tagatgccaa tgtaaatatg gaactggtca tggagctttt
    20221 acttctgacc atgaaagaga attaatagaa ttgtatttta agggtgtttt aggaaaggaa
    20281 attaagtaag agaaatgtta acagtaatcg gagtgcgttt tcgggctgcc ggaaagatct
    20341 actattttga tcctgcagac cgtcagataa aaattgggga ccatgtgatc gtagaaactg
    20401 ccagaggtat tgaatacggt tacgtagttc ttggaaacag agaagtagat gagacaaagg
    20461 taataccacc tcttaagccg gtgatccgta tggcaacgga tgaggaccgt gccattgagg
    20521 caaaaaacaa agagaaagaa aaagaagcat ttaagatctg ccaggaaaag atcaaaaagc
    20581 acaacctgga aatgaagctg atcgatgcgg aatatacctt tgacaataat aaggtgctgt
    20641 tttactttac agcagacgga agaattgact tcagagagct ggtaaaggac cttgcaagtg
    20701 tatttaagac ccgtatagag ctgcgccagg taggtgtgag agacgagacc aagatcatgg
    20761 gtggtatcgg catctgcgga agaccgctgt gctgccattc ttatctgtct gagttcatcc
    20821 cggtttctat taaaatggca aaggagcaga acctgtcctt aaatccaact aagatttccg
    20881 gcgtgtgcgg aagactgatg tgctgcctga aaaatgagga agagacctac gaggtattaa
    20941 acagcaaact gccgggaatt ggtgatacag ttaccactgc agacggcctg aggggagaag
    21001 tacacagtgt aaatgtgctg cgccagactg taaaggtgat cgtagtagta gataaagacg
    21061 aaaaagagat ccgtgaatac aaggtagacc agttaaagtt taaacccaga agaaagaagg
    21121 gcaaaggcgg cgaaaagcag gacgaagcag agttaaagaa gctggaagca ctggaaaagc
    21181 gggaaggaaa gtcaaggttg aatgataaat agtgaaatgg aacacagctg gagacaggaa
    21241 ggaaatatcc ttttaaggga tgatgagcgg atcgatgatc ttcagagaaa tcattacggc
    21301 atcatccaga gaaaaggcac cttctgtttt gggatggatg cggtgctgct gtccggtttt
    21361 gcagtggtaa agccaggaga aaaggcgctg gatctgggaa caggaaccgg tattatcccc
    21421 attcttttag aagcaaaaac agaaggaagg cactttaccg gccttgagat ccaggaagag
    21481 tctgcggata tggccaggag aagtgtgaaa tacaatcacc tggaagaaaa ggtggagatc
    21541 gtaacaggag atatcaagga ggccggaagc aggtttgcac tggcttcttt tgatgtagtc
    21601 accagcaatc caccttatat gaatgatgcc cacggcatta aaaacccggg agatgccaag
    21661 gccattgccc gccatgaggt gctttgcacc ttagatgatg tggtaagaga aggcacaaga
    21721 gtgttaaaac cagggggacg gttttatatg gttcaccgcc caagaagact gatcgagatc
    21781 ttacagacta tgaagcagca tggtttagag cctaaacgga tgaaatttgt acatccctat
    21841 aaggaccggg aagccaatat ggtgctgatt gaggcagtaa gaggcggcgg agccctgatg
    21901 aaggtagaag cgccggttat tgtatttgat gagaacgggg agtattcaca ggagatacgg
    21961 actacttacg ggtattaaaa agcgcttgcg gaggaaaggt acatgacagg aaaattatat
    22021 ctgtgtgcaa cacccatcgg taatctggaa gacattacct accgggtgtt aaaaacatta
    22081 aaagaggcag atctgatcgc agcggaagat acaagacaca gtatcaagct gttaaatcat
    22141 tttgacataa aaacgcctat gacaagttat catgagtata ataaggtgga aaaggcaaaa
    22201 tatctggtag agaaaatggc agagggcacc aacattgccc tgatcaccga cgcgggaaca
    22261 ccgggaattt ctgatccggg agaagaactg gtaagacagt gctatgaagc aggtattccg
    22321 gtcacttccc ttccaggacc tgcagcctgt atcaccgccc ttaccatgtc cgggcttcct
    22381 accagaaggt tctgctttga ggcatttctg ccagcagaaa aaagtgataa aaaagagcgc
    22441 cagcggatcc tggaagaact gaaaaaagag accaggacca tgatcgtcta tgaagcgcct
    22501 catcatctgg tggcaacttt agaggacctg tacggggcac tgggaaaccg gaagatcacc
    22561 atctgcagag aacttaccaa gaagtttgaa agtgccttcc agacaacctt tgaagaagct
    22621 cttgcccact atgaaaccga agagccaaaa ggcgagtgtg tcatcgttat cgccggaaag
    22681 ccggtagaag agatccggga agaagagatc cgcgcctggg aagagatgcc tattgaagag
    22741 catatggagc attatctgtc acagggaatg gataaaaaag aagccatgaa ggcagttgca
    22801 aaggacagag gaatatccaa gagagatgtg tatcagcagc tgctgtaata aaatgtgcgt
    22861 gatgctgaaa tattcgctca tgttttgtgg gattgagcca gatattcgct catgttttgt
    22921 gtaatgaatt tagaagtaat taaaaaaact gccgtaagac cacggatttc atggtcttgc
    22981 ggcagtttta attgttttta tgtaatttac tcagctaact tcaaaccggc cagctttgcc
    23041 agcgccagaa tggatgcctt agaaactttc tttccttcat attcaaccag atcttctgtt
    23101 cccccggtga agatatctgt gtttgctgct ttttttagga tgatgcagtc atcctggact
    23161 gtgatctcaa gggcgtcttt tacgtccagg ttcaggcttc ttctcagctc gataggaagg
    23221 gtgatccgtc caagctcgtc cagttttctt ataacccctg ttgttttcat aatgaaatac
    23281 ctcctgtttt gttgtacttt gcgaacccca taccgctgcg atccctaact taagggacat
    23341 catgcattgt ttacaatgta tagttaaaaa gtagcaaatt ttggaaatta agtcaatgat
    23401 aaagggggga ttataaaaga atacaggaaa atactgaaca gaaacgaaaa aacagtggat
    23461 taaatattaa aaaaatattt cttgacaaat agcatctctt cctgtagtat tactttgaag
    23521 ttcaaagtat ttctgcggaa aggtaagaaa aatgactaca aaaattatag gaactggttc
    23581 ctatgtaccg gctcagatcg taacaaatga tgacctgtca aagatcgtcg aaaccaatga
    23641 ccagtggatc cgcagccgca cggggatcgg agagagaagg atcgcaacag acgacagtac
    23701 atcagatatg gcggcgaaag cagctgcgag agccattgag caggcgggaa ttgatccaga
    23761 ggagatcgac ctgatcatcc tggctacttc atcaccggat tactgcttcc caaatggagc
    23821 ctgcgaggta caggcgaaag ttggcgcagt gaatgcagcc tgctttgata tcagttctgc
    23881 atgtactggt ttcgtgtttg ccttaagcac tgcacatgcc tatatttctt ccggtcttta
    23941 taaaacagcc cttgtgatcg gcgctgacct gttaagcaag ttagtagact ggacagacag
    24001 aagcacctgc gttctctttg gagacggagc aggagcagca gtggtaacag cagctgaaaa
    24061 tggtatcatc ggtctgaaca tgcattccaa cggagccaaa ggcggggtac tgacctgtgg
    24121 aagccgttct acgggaaact tcttattagg aaagaaaccg gagcttggct acatgaccat
    24181 ggaaggacag gaagtgttca aatttgctgt aaagcaggtt ccagagtgta tcaaagaagt
    24241 tttggaagaa accggaaata cagcagaaga tattgaccag ttcatcatcc accaggcaaa
    24301 ttaccgcatc attgagtcca ttgcaaaacg cctgaaagtg gatctggaga aattcccggt
    24361 gaacatggaa cactatggaa atacatccgg cgcgtccgta cctattttac tggacgagtt
    24421 aaacagagcc ggaaagctta aaaacggaga caagctggtg ttagccggat ttggcgcagg
    24481 tcttacctgg ggagctgttt tactggagtg gtaaacagac agtaataaat aacgtaatat
    24541 acacgcagtg gctttgccgc agcggataag ttaaataaaa atataaattt taatcagaaa
    24601 aaggagaaca atatcatgtt agagaagatg aaagaactta ttgcagatca gttaagcgta
    24661 gatgcagaca gcatcacaga ggcttcctct tttaaggacg atttaggagc agactccctg
    24721 gatctgttcg agctggttat ggctttagag gatgagtatt ccgtagagat ccctgcagag
    24781 gacttagaga acctggcaac tgtaggcgat gttatgaact acttaaagaa caaaggcgtt
    24841 gaggcttaat tataagtaga catgctgcca ggcggcggca atgaaagtca gctgctccgc
    24901 gaaaaagcgg agtatacatc aatagcaaca gggggacggc gtgcttacta tggaagcacg
    24961 ctgtttctac aaggctacgg gaaaatcccg gaattttgga ggaaagaatg aatacaagaa
    25021 ttacagaaat gttaggaatc gaatatccca tcattcaggg aggaatggca tgggtggcag
    25081 agcacaacct ggctgcagcc gtatctgagg caggcggtct gggactgatc ggcggcgcaa
    25141 acgctcctgg cgaagtagtc agagaagaga tcagaaaggc aagagagctg acaaagaagc
    25201 cttttggtgt caatgttatg ctgttaagcc cacatgcaga tgacgtagca aaagtagttg
    25261 tagaagaagg catcaaggta gttaccacag gtgccggaaa ccctgaaaaa tatatggata
    25321 tgtggaaggc cgcaggcgtt aaggtcattc cagttgttgc atctgttgct ctggcacgcc
    25381 gtatggaaaa atatggagct gacgctgttg ttgcagaggg aatggagtcc ggcggacata
    25441 tcggcgaaga gactaccatg actttagtac cacaggttgc tgacgctgtt tctatcccgg
    25501 tgatcgcagc aggcggtatt ggtgacggaa gaggcattgc agctgcattt atgttaggtg
    25561 cagaggcagt acagatcggt acccgcttcg ttgtttccaa agagtctatt gtacatgaaa
    25621 attacaagga gcgtatcatt aaggcaaaag atatcgactc cactgttaca ggaagaaccc
    25681 atggacatcc ggtacgctgt ctgagaaacc agatgaccag agaatacatc aaattagagc
    25741 aggaaggaaa gtcctttgag gaactggaat acctgacttt aggaacttta cgcaaggcag
    25801 ttcaggaagg cgatgtaaag aacggaaccg ttatggctgg tcagatcgca ggtcttattt
    25861 ccaaagagca gacatgtaag gaaatgatcg aagagatgat gggtcaggca gagaaacttt
    25921 taggtcgttg ttaggtactt tttgaaccgc ttttaaacag ggagtaatga atatgagtaa
    25981 gatcgcattt atcttcccag gtcagggcgc acagacctgc ggcatgggaa aagattttta
    26041 tgagcagaca gaaacaggcc gcaaggtatt tgacaaagcc acagagcttt taggcttctc
    26101 tgttccggag ctttgctttg agccgaatga cagactggat attacagaat atacacaggc
    26161 agctatggtc accaccagca ttgccatgat gaaggtttta gaagaaaatg gcataaaacc
    26221 agacgtagca gctggtttaa gccttggcga gtactgtgca ctggcagcag caggggttat
    26281 gagcgaagaa gatgccatta caacggtacg tcagagaggc atcttcatgc aggaagaagt
    26341 tcctgctgga gaaggcgcta tggcagccat cctggcatta gatgcagcag tgatcgaaga
    26401 agtaactgct gatatagacg gtgtatggat cgccaattac aactgtccgg gacagatcgt
    26461 tatttccggt aagaaagcag cagtagaaga agcctgtgag aagttaaagg cagcaggtgc
    26521 aaaacgtgcc attatgttaa atgtaagcgg tccattccat tcaggaatgt tagttggcgc
    26581 aggcgagaag ctgggaaagg ttcttgaaaa tgtagagatc cacaagccgg ttatcccata
    26641 tgcagctaat gtgactgccc agtatgtaac agaggcagaa cctgtaaaag atcttttagt
    26701 aaagcaggtt tcttcttctg taaaatggca gcagtgtgtg gaaaccatgc tggcagacgg
    26761 tgtggatact ttcattgaga tcggaccagg aaagacactg gctggcttta tgagaaagat
    26821 cgaccgcagc gtaaaaacat taaacgttga gaagttagag gatatagaaa aggtagtaga
    26881 agctttaaaa gaggcgtaga aggagaataa acatgctgga aggaaagatt gcactggtta
    26941 ctggcgcaag ccgcggaatt ggacgccaga tcgctaaaac attagcggca aaaggcgcat
    27001 ttgtgatcgt aaattataat ggttctgctg caaaggcaga ggaagtagta aaagaaatcc
    27061 aggcagccgg aggaaacgga caggcagttc agtgcaatgt ttccgacttt gaaagctgca
    27121 aggaaatgtt agatgcagta gtgaaagagc atggacgtct ggatatcctg gtaaataacg
    27181 caggtatcac aagagacaac ctgttaatga aaatgtctga ggaagacttt gacgcagtga
    27241 tccagaccaa cttaaagggc gtgttcaact gcacccgtca cattgcaaga cagatgttaa
    27301 agcagaagag cggccgcatc atcaacattt cttctgtatc cggtgtatta ggaaatgcag
    27361 gacaggcaaa ttactgtgca gctaaggccg gcgtcatcgg actgaccaag agtgcagcaa
    27421 aagagctggc aagccgcggt attactgtaa atgcagttgc accaggattt attaagacag
    27481 aaatgacaga tgtgttaaaa gatgatatca agaaagcaat tatggaaaac attccgatga
    27541 aagcttttgg tgagacggaa gatattgcaa atgcagttgc gtttttagca tctgaggaag
    27601 cacgctatat taccggtcag gtaatatctg ttgatggagg catggctatg tagtaaaata
    27661 gctttcgctg tttcgttaca ccgccaatca ggcaaaaata gaacaggagt caaatatatg
    27721 aatacaagag tagtagtaac aggtatgggc gctattaccc caattggaaa tagtgtagag
    27781 gctttttgga ctgcagtcaa gtcaaataca gtaggcattg caccgatcac tcattttgat
    27841 acagctgatt ttaaatgcaa gctggcagca gaagtaaaag attttgatcc aaagcagtat
    27901 atggatccga aaactgcacg tcgtatggaa acattctgcc agtttgcagt agcagcggca
    27961 aaggaagctc tggaaacatc aggcattgac atggaaaaag aagatccttt ccgtgttggc
    28021 gtaagcgtag gatccggtat cggttcttta caggcgttag agcgtaatga aaagagactg
    28081 ttagaaaaag ggcctggcag agtagagcca ttattagtac ctttaatgat cagcaacatg
    28141 gcagcaggaa atgtttctat ccagtttggt cttaagggaa aatgcatcaa tgtagtaact
    28201 gcatgtgcaa ctggtaccca ttccatcggc gaagcattcc gttccatcca gcatggtgag
    28261 gcagatgtaa tggtagcagg cggtacagaa gcaagtatta ctccaattgg tgtggctggc
    28321 tttacctcct taactgcatt aaacaccacc gatgatgtaa agaaagcatc tatcccattt
    28381 gaccaggaca gaaatggttt cgttatgggc gaaggcgccg gtatcgttgt attagagtcc
    28441 ttagagcacg cacaggcaag aggggctaag atcctggcag aagtagttgg gtacggtgca
    28501 acctgtgacg cataccacat tacttcccct gcagaagatg gaagcggcgc tgcaaaggca
    28561 atggaatttg ccatgaagga tgcaggaatt accccagagc agcttgatta cgtaaatgct
    28621 catggaacca gcacccatca caatgactta ttcgagacaa aggctattaa actggcttta
    28681 ggcgatcatg caaataaagt aaagatcaac tccaccaagt ccatgatcgg ccacttatta
    28741 ggcgcagcag gcggtgtaga gttcattacc tgtgtaaaga gcattgaaga cggctatgta
    28801 catccaacgg taggtctgga aaatccaggt gaaggctgcg acttagatta caccatgcat
    28861 gagggcgtat ctatggatgt aaattatgcc atcagcaact ctttaggctt tggcggacat
    28921 aatgccagcc tgatcgtgaa gaaattcact gcataaggaa ggagaacgtg gacatgaata
    28981 tggatgaaat cctgacactg gttcaggctg tatctgattc aaagctgacc agctttaaac
    29041 tggaccaggg cgatatgcat atttccctta agaaagagaa agaagaagca aagatcatca
    29101 ccgttggcgc acctggcgct gtagcagcag ttccggctcc aagtgcagca cctgtagtca
    29161 ctgcagcttc cgcaggagta agcacagaag cacctgcagc tgcaccggaa gcagcagaaa
    29221 gccatgacat tgcttctgat aaagtgatca cctccccatt agtaggaacc ttctacagtt
    29281 cttcttcccc ggatgcagaa agcttcgtaa aagagggaga tactgtgaaa aagggacagg
    29341 tacttggcat tattgaagca atgaagctga tgaacgagat tgaaagcgaa tatgacggcg
    29401 ttattgaagc gatccttgta aataatgagg aagtagtaga gtacggacag ccattattcc
    29461 gtattaaata agatgtcctc ctggaacctt tccaaataaa aaaggttccg ggagaacatt
    29521 ggttccgaga gaaaatagga gggcaacaac catgttaatg ggtattaaag aaatccagga
    29581 gatcattccg cacagacatc catttctcct ggtagactgt attgaagaat tagagccggg
    29641 cgtaaaggca gttggctaca aatctgttac ttatgatgag ccatatttca gaggccattt
    29701 cccgcaggaa ccggttatgc cgggcgttct gatcgtggaa gcactggctc agaccggcgc
    29761 tgtagcgatt ctttcccagg agaatttcaa gggaaagact gcatattttg gcggcttaaa
    29821 taacgtaaaa ttcaagcaca aagtagttcc gggagaccgt ttaagactgg aatgtgagat
    29881 catcaagcag aagggaccgg ttggcgtagg aaaagcaact gctacggtag acggcaagat
    29941 cgcagttgtt ggcgaactga cctttatgat cggatagata atactggtcc ataggagatt
    30001 tgaaatgttt aataagatat tgatcgcaaa cagaggtgag atcgcagtcc gcatcatcag
    30061 agcctgccgt gaaatgggca tccgcaccgt tgcagtttac tctgaggctg ataaggattg
    30121 tcttcacact ctcctggcag atgaagccat ctgtatcggc ccggctcctt ccagccagag
    30181 ctatctgaat atggagcgta ttctctccgc aactgttgca atgaaggcag atgccattca
    30241 tccgggattt ggatttttat cagaaaatgc ccgttttgca aaactttgcc agcagtgcaa
    30301 cattaccttt atcggaccgt ctgctgagat catcaaccgc atgggaaata agtcagaagc
    30361 cagaaatacc atgatgcagg ccggagttcc tgttgttcct ggaagcaaag agcctgtata
    30421 cacagcagaa gatggacttg ccatggcaaa agagatcggt tttccggtca tgatcaaggc
    30481 ttcttccggc ggtggcggaa agggaatgcg tatttccaga agcgaagagg actttacaga
    30541 gcattttaat gcagctcagt tagagtctgt aaagggattt tccgatgata ccatgtatat
    30601 tgaaaaatat attgaaaaac cacgtcatgt ggaatttcag atcatgggtg acaaatttgg
    30661 ccatgttgtt cacttgggag aacgtgactg ctccatccag cgccgtcatc agaaggtaat
    30721 ggaagagtcc ccttgtgagg tcatttcccc tgaacttcgt aaaaaaatgg gtgaagttgc
    30781 agtaaaggca gccaaagccg tgaattacga aaatgcagga accattgagt ttttgttgga
    30841 taaagacaaa aatttctatt ttatggaaat gaacacccgt atccaggtag agcatcctgt
    30901 aacagagatg gtttccggca tcgatctgat caaagagcag atccgtgttg ctgcaggaga
    30961 gccattaagt gtgtcacagg aagacatcca gatcaaggga catgccattg aatgccgtat
    31021 taatgcggaa aacccgaaaa agcactttat gccatgtccg ggccgcatta ccaacgtgca
    31081 tatcccaggt ggaaatggtg tccgcatgga tacacacatt tacaatgatt ataaagtacc
    31141 tgccaactat gactccatgc ttatgaaact gatcgtttac gataaagaca gggcatctgc
    31201 cattgcaaaa atgcgcagtg ctttaggcga ggtcatcata gaaggcattg aaacaaatat
    31261 tgattttcag tatgagatcc tggaaaatga ggcttttcag aagggtgata cagacaccgg
    31321 atttattgaa aagtatttcc cggactacat aaaatgattt taatctggaa aaatcctccg
    31381 cgaccaggtc gcggacggat atttatgatc ctgaataagg ataatgcaga aaggaagttg
    31441 tcgtatgctg cgcaacatgt tcagaaaaac gtatacaaaa atagatacca aatacagacc
    31501 ggtaagccat gaaggagaag aaccatccat cccggaaggc ttatggcgca aatgtaataa
    31561 atgcgggcag ccgatctatg tggaagacgt aaagaacaat tactatgtat gccccaaatg
    31621 cggcggctac ttccgtgttc atgcttaccg caggatcgaa atgcttaccg atgtaggaac
    31681 ttttgaggaa tggaataaag aaatgccatt ttccaatcct ctggatttcc caaattacga
    31741 aaaaaaagta gaagcagcca gagaaaaaac aaatctgaat gaagccattg ttattggaaa
    31801 ggcaaagatc gacggaaatc ctgctgttgt aggtgtctgt gacgcccgct ttatcatgag
    31861 cagtatgggc catgtagttg gggagaagat cactgaggca gtagaacgtg ctaccaaaga
    31921 aaaacttccg gtgatcctgt ttgcctgctc cggcggtgcc agaatgcagg aaggcattgt
    31981 ttccctgatg cagatggcaa agacttccgc agccttaaag cgtcaccatg aagcaggaca
    32041 gctgtttatc agtgttctca ctgatccaac caccggcggt gtgacagcaa gttttgccat
    32101 gttaggtgat attattattg cggaaccagg tgcattgatc ggctttgcag gcccaagagt
    32161 cattgagcag accattggac agaagctgcc ggaaggcttc cagagagcgg aattcctgtt
    32221 agaacatgga tttgtagaca tgatccttcc acgtcaggac cagaagcatg tgatcggacg
    32281 gatcctttac atgcatagaa aacatgatat ggatgtggaa gagaacgggg ttaagacaga
    32341 tatacctgta aataccctgg cagctgcaca gtctcaggaa aacacagaaa agagtgcctg
    32401 ggatacagtc cttctttcca gaaaggcaga cagacccacc gctcttgact atatcaccgc
    32461 tgtttttgat gaatttatgg aattccatgg agaccgctgc tttaaagatg acggagccat
    32521 tgttggcggt attgccatgt tccacggcat gccggtgact gtcatcggac agcagaaggg
    32581 aaagaatacc aaggacaata tccgtcgcaa cttcggtatg ccgtctccag acggctaccg
    32641 caaggcgctg cgcttaatga agcaggcaga aaccttcgga cgccccatta tctgctttgt
    32701 agatacaccg ggagctttct gcggcctgga agcagaggaa agaggtcagg gagaagccat
    32761 tgcaagaaat ctgtttgaaa tgtcggattt aaaagtaccg gtgctttcta tcgtcatcgg
    32821 cgaaggcgga agcggcggcg cgctggctat ggcagtagca aatgaagtat ggatgatgga
    32881 aaatgccatt tattccattt tatcaccgga aggctttgcg tctatccttt ataaggacag
    32941 taaaaaggcg ccggaagcag ccagagtcat gaaagtgacc gctgctgatt taaaggaact
    33001 gggactgatc gagcggatca taccggagga agaaccggca aatacagata ctctgtaccg
    33061 cattgcaggt tatatggatc ggtctatggt gaaatttatg aagaagtatc aggatatgtc
    33121 aggagaagag ctggcagagc acagatacga aagatttagg agaatgtaat gagtgggatc
    33181 aaagctttaa aaataggaga tctggtactg caaagaccag tgatccaggg cggtatgggc
    33241 gtaggcatca gccttcataa actggcggga gctgtagcag cttccggcgg catgggactg
    33301 atctccacag cacagatcgg tttcagggaa cctgatttta agaccaactt tgtagaggca
    33361 aatctgcgcg ctatcaggcg ggagatgcag aaagccagag agattgcgcc aaaaggagct
    33421 atcggtttta atattatggt agccaccaag cactatgacc tgtgggtcaa ggaagccatt
    33481 aaatccggcg cagatgccat tgtatccggc gcaggtcttc cggtacgtct tccggaatat
    33541 gcacaggctg cttacgaaga gatgaaagcc ggtattgcag acgcaaaaga aaactgcgaa
    33601 gaattttgca agcaggcagt aaaaaaagta aagcttgccc ccatcgtatc ttccgcaaag
    33661 tctgcacagg ttatctgcag actttgggac agaaaataca agcaggttcc tgattttgta
    33721 gtagtagaag gacctttagc cggtggtcat ctgggctttt ccagagaaga actggctgag
    33781 tacggcgcag acaccaaaga tgtgccaaat acctataacc aggaagccta cgataaagaa
    33841 gtccgctcca ttatggaagt ggtaaagact tacgaagaaa aataccagaa acatatcccg
    33901 gtagtaactg ccgggggaat ttatacccat gaagatgtaa aacaccagtt tgaactgggg
    33961 gcagaaggcg tacaggtggc aactcgtttt gtcacgacag aagaatgtga cgctccggat
    34021 gcttacaaac aggcttacat caacgcaaaa aaagaagata tcgttatcac ccagagcccg
    34081 gtaggaatgc caggacgtgc catcttaaat ccgtttttac agcagatcaa agacggcgtc
    34141 cgtccggcca tcaaaacctg cttccagtgt ctggaacact gcgatatcaa aaccattcct
    34201 tactgcatca caaaggcatt ggtaaatgca gcagaaggag acgaagacaa cgcccttctc
    34261 ttctgcggca gcaacgctta cagagccgaa aagattgaaa aagtagatga tgtgatgaaa
    34321 gaactggtgg gagaattgta acgcatgaac cgcctgcgta agcagacaga taagcatgtt
    34381 ggtacgagtt cgcgagtccc cccaaaaata ccggtcatac ccataaaaag gtatggccgt
    34441 tttttcttac aaacccttac aaaatcctta cttgccttga ccttggagta acttcaaggt
    34501 gtatgctttc tgcataaaac aaaaggacat gttccatgag ggggacgttg tcatatataa
    34561 aaacgcagga ggcatttaat tatgaagaaa cgttttgcta ttacagtact tgcgctggct
    34621 ctgacggcag gaagcgctat gacatctttt gcagcaggtt ttacaggtac aggaaaaggc
    34681 gtaaagtatc agtggggtga tggagcttac tgcaccaata actgggtaca gtataaaaat
    34741 cactggtttt attttggcga tgaccagtta atgagaaccg gttggatcca gaaggacggc
    34801 acctggtatt atgcggctga taccggagaa cttcagggtg gtattatgaa gatcaacgga
    34861 aatgtttatt attttgatac cagtacctgc aaaatggtaa cgggcaacta cagctataat
    34921 ggcggaactc atgaatttac agaaaacggt accacagatg gcggaccata tgtttacaat
    34981 gaatggaaca gcaacggtat catcaaacgg ggaaccaaat ttggagtaag ataaatatcc
    35041 agataaaact ttacagtttc ctttcgattt tctgcgtccc attgaccagc tcgcaaaatc
    35101 cggataagat aacattcata aacacattcg gaagaaatgt tgatcgtgag aaaatgggaa
    35161 aacaggagac tggaaaggca tggcagaaaa cagaacggaa ataaaacagc gagagtatct
    35221 cataggcgat atgtccgaaa ttgtgggcgt aagccgggat actctccgtt tttatgaaaa
    35281 aaaagggatc ataaaggcga gaaaaaagga aaacggatac cgttattatc tggaagaaga
    35341 cctgttcaga ctgatggtta tcctatataa gagaaaaatg aatgacagcc tggaagaaat
    35401 cgaaggatac ataaaaggcg agaattctgt agaatatatg aaaaaacatc tgcagacccg
    35461 tataaaacag gaagagatga agatccacta tcatcagcag gccattgccc gtatgcggct
    35521 tgccacaaaa gatattcaaa tggcagagca atacggtgga aaatgtacct ggaaaaattt
    35581 tccggtagcc tatcagttag gagtttgcga cagtctccac gcaagtctgc aaaaatggtt
    35641 ttccctgtca tctgaaacag gcgggttgga catgacttat ttctataccg aattttccat
    35701 aaaagaaggg cagatgcagg agcagggcac caagctgttt ctgtatcagg aagtaggtcc
    35761 ctttatcagc agtgacttcc atctggaaca atatccgcag actgcacccg ttccctgtat
    35821 ttatacgatc ctggagtctg aaaatattta tcccgatgaa gaaacagcct gccagatggt
    35881 ccgctgggga aaggaacagg gagcagagcc aacaggagtt ctgtattcca ataatatgac
    35941 ttcgttttta ggagaagaca gcaacattta tttcctggaa ctgtatatgc cggtaaaata
    36001 aaaacagtaa aacggcaaaa caagaaaaag caggaaaaaa caaggaaaag cccaaaagga
    36061 aaccgcaaaa tctgccaaaa tgtggataaa aaatccacaa aatttccggt ttccacataa
    36121 aaatccatat ttccccacag tttccccatg ctttacacac actataaaca gagttatccc
    36181 caaagttatc cacattatcc accgacaaac acaagatatg tggtaaaaag aagagaaaat
    36241 acaagattta gattgtggat aactttttcc accataagaa tgtgtatttc tctgtgtgaa
    36301 agagcataaa atgtgtataa atgggcgtat aggcaataca ggaaaaagga atatgttaaa
    36361 ttatatctgg agcggaatgc tttttctggg gatcatatgg ggcacgtttc acggaaggct
    36421 ggctcaggta acagaaggag ccttaaacgc agcaggggaa gccgtgacct tgagtatcac
    36481 catgctgggg atcgtagttt tctggaacgg gatcttagag atcggaagca gagccgggct
    36541 ggtagaacag ctttcagaaa aaatgagacc ggctttgaaa ttcctctttc cggatctgcc
    36601 gccagactgt gaagccgcca gacagatctg tgtaaatttc attgcaaata tcctgggact
    36661 tggctgggcg gcgacacctg caggcttaaa agccatgaaa gaactggaaa aggtagaaga
    36721 agagcgcaga aaagagaaag tggcgaaaag gacaattcac gcagtctcga aaggcactgc
    36781 cagtaatgaa atgtgtacct ttctgatcat gaatatttca tcccttcagc tgatcccggt
    36841 gaatatcatt gcatacagaa gccagtatca cagtacatcc ccggcagccg tcaccggtcc
    36901 ctgttttgca gcaacaatgg taagtacact ggcagcggtc attttctgta agatcatgga
    36961 tcatctccgt aaccccacat agatcttccc caaaatgctc acctcatttc gggtatatcc
    37021 taataaaaaa ggagacacca taatcagtgt ctccttcggt ctaactgccg gttcttggca
    37081 taataagcac gtttatcttc atacattctg gcgtctgcct cagacagcga tttttctact
    37141 tttccggtgc agtcatccac ccagacagaa ccaagtgcca ggtgaaccga acttttagcc
    37201 atgtcaacta acatagaacg gatcatagac tgaaaaagat tttctgtaat acccggtatc
    37261 agtaccagaa attcatcacc gccaatacgg taaacacatt tatccggaaa atgagccttt
    37321 aaattttcac aggcatagat caacgcctca tcacccgcct ggtgtccaaa atcatcgttg
    37381 atctgcttta agcccattac atcactgtaa ataatgccaa gacttccacc gtttgcaaga
    37441 gaagccagct tttcgttcat ggcatgacgg tttttcgcac cggtaagctg gtcatggtag
    37501 cttaatgttt ccagtttccc aaccaggtct ctgcgccgca gcatggaatt gataaaatgt
    37561 ccaaggagct ggagcataaa agcaatatga tccatacggt caagtggcgg attgtcaata
    37621 ccatagaaac cgattacttc ccggtcacgg taaatagggc tgacaataag gcgcgcaatg
    37681 ttttgcggta tcagtgtttc atatacagta gggtcataag atacgatctc atccacattc
    37741 cggatgataa tactttcacc tttccggaag gtaggaatcc aggtacccat ggtttccggc
    37801 gggatattgc gtaaaatatt tttctgaggg gaaaccttag aagcacacca ttcataagta
    37861 ttgtcaaaat tgccatgttt atttttctca aaaatataaa cacggtcgct ttggaacatc
    37921 tgccccagat actgtaataa tgtatcaata gactgttccg gtgtttcttc tgccagtgca
    37981 cagcgaagtc cctcattgat cagtgcttca ttgtctgtaa attcagaaaa agaacgttta
    38041 gccagttcat gaatatccag gtcaattgcc atttcaatac ggactctgcg tccatcgtca
    38101 attaccatgg tatctttcag cagataagaa cggcgcacca gcgggttaaa acgggaccat
    38161 tcataaaaca caccgggttt taagcggttg ttggtgcaca tggcacaggg ggaagaacat
    38221 tgctgcagga tctcatagca gttttttcct ttataatcag actcatcctt aaactgaaac
    38281 agatctctgg cagctttatt caaatagagc agttcgtatg tatccacatc agaggcataa
    38341 acagcctcat tcatattttc aaaaaattcc caaactttag acataaaaaa tcccccgtgt
    38401 tgcataactg tatttattat agctgactgt ttagaagaat ggtaggagaa cttttagact
    38461 ttcttcgaca aaagttaata tttttataat gggataatgt tataatcaaa gaatgagagt
    38521 aaaacttaaa aatgcaaagc agagaaagga agaaaataat aaatgagaca gaacgggaat
    38581 aaaatctggc atataaaaaa taagagatct ttaaaattag cggcagctat gcttgcagtt
    38641 tcgctggctt ttcctatggc agcccaggca aaagcatggg actttgaaaa tggtagttat
    38701 gtagatgctt ccggcactcc cattgaaggg gcagtagcaa aaggtatcac cgtgacaaaa
    38761 tatcagaacc gtgcaaaccg ggaaaatggc attgactggg gaaaagtagc tgcagatggc
    38821 atcggttttg ccatgatccg tattggttat tataaggata aagatccgta ttttgaccgg
    38881 aatataacgg aagcagcagc tcatggtata aaaacaggtg ttttcttcta tacacaggcg
    38941 ttggatgcac agacggcagc agatgaagca gaatatgtgc tccaggttat caaggatttt
    39001 ccggtatcct atccggttgc ctatgatgtg gagtccaatt acctgctgga acagggctta
    39061 agttccgccc agatcacgga aaatgtcaat gccttctgta aagtgatcgc agatgcaggt
    39121 tactgtccca tcgtatatgc aaataatgac tggctcacta atcacttaaa tacagcagaa
    39181 atcccatacg atatctggta cgcccgctat ggcaccatta acagctatcc aaaccgtacc
    39241 atctgg
//
LOCUS       DBMDUA010000006        38194 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_898_length_38194_cov_3.507022, whole genome shotgun sequence.
ACCESSION   DBMDUA010000006 DBMDUA010000000
VERSION     DBMDUA010000006.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 38194)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 38194)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38194
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_898_length_38194_cov_3.507022"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            411..1247
                     /locus_tag="ACZPCL_01060"
     CDS             411..1247
                     /locus_tag="ACZPCL_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013577.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1161112.1"
                     /translation="MLQELYEQYKDTFSKSDHKLIQYLLRNEEQLQYLTSEELSAHTG
                     ISPATVSRFWKKIGFQNLKELKIKQRIQDTATPSSRLTSALKRFEETASLTEDLKMHF
                     GNNTAKTLDRMDPALPARAADLLLQAQHVYIYAPDASCGLASIFCYRARRLGIQPVLL
                     EGGSAIYEYMVNITGNDLVLLFSFSRLLGETRILLDHCNKINCPVILFTDLFATQEEM
                     PSRLTFYCYRGEPNEYHSMTVPLLVLDLIILNLMQASKNSLTSSHYLEKLRAEYAQII
                     RR"
     gene            1349..2029
                     /locus_tag="ACZPCL_01065"
     CDS             1349..2029
                     /locus_tag="ACZPCL_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HIR1161113.1"
                     /translation="MKKPLAVLITAFSLSLGLTPAFTGISGNLIIEAEAHQGRTDANG
                     GHHDYKNKSGLGSYHYHCGGNPAHLHPNGVCPYAGGSSGNSGSAGGSSSKNPSSSGNQ
                     NQTETAAAQVSTGWQKDDHGWWYRLSADTYYADGIADIDGATYLFNSDGYMLTGWQQL
                     NGHWYYFNASGAMVTGGLTIDGKYYYFDSDGVLDETDYDAVDEPGDETSDDDTEYDYD
                     YDYDLEEY"
     gene            complement(2268..3191)
                     /locus_tag="ACZPCL_01070"
     CDS             complement(2268..3191)
                     /locus_tag="ACZPCL_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HIR1161114.1"
                     /translation="MSKKTLEELVFHDDFMFAAVMMDAENCRCFLERVLEIQIERVEI
                     STEHGFFFNPECKSIRMDVFAKDENRTHYDIEMQLVKKDSLEKRSRYYHSQMDVEMLE
                     KGKSYGELADTYVIFICNFDPLGQKKCRYTIRRYCEETGNVFGDGVCTVFLNTNGKNR
                     EEIPKELVALLDFVKADLAGSEADYEDALVKRLQESMRQIKRSRKMGERYMMFEQYMK
                     EYVQEHADEIREEAWAEGLAEGHAEGLQKGRAEGLQEGRAEGLQKGRAEATTRLNKLY
                     SCLLTSDRADDLQRALKDPVYLEQLLQEFGF"
     gene            3422..4378
                     /locus_tag="ACZPCL_01075"
     CDS             3422..4378
                     /locus_tag="ACZPCL_01075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1161115.1"
                     /translation="MKFAVTIVTYNRLELLKECIQQVLSQTVPFSYICIVDNHSTDGT
                     SEYLDQLAAGTETVFPNAGKPEFHILHLPENIGGAGGFAKGLEDLGKTDCDWILIIDD
                     DAMIAADYIEQLQKAILKTNYLAYSGTVTTAGAIDTTHRRFIQCPRLMFYKPVPEEEY
                     AKASFEYDISTFCGLLVNADMVRKIGLPKTEYFIWFDDTEYCLRFHDQSRILNVTTAV
                     LNHKTAPVGSAPVISWKNYYGFRNAIDIGKTYSKNPHLYMAYIWLNHKAHIFLDTFGL
                     LLGNQKEMRRYRIKVYKDVLKNRHKKPDGISPDYLPGSGYKS"
     gene            4392..6212
                     /locus_tag="ACZPCL_01080"
     CDS             4392..6212
                     /locus_tag="ACZPCL_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1161116.1"
                     /translation="MQAIILAAGMGKRLKQLTQNNTKCMVKVNGVPLINRTLNQLEQH
                     NLSRIVIVTGYEGQKLREHIEGLHIKTPVVFIDNPVYDRTNNIYSLFLAKDYLLKEDT
                     LLLESDIIFEDSVLNSLTDDPRDSLALAAKYESWMDGTCLVLSDQDDIEAMIPGKKFR
                     FSDTEHYYKTVNLYKFSQEFSRTHYVPFLEAYTKALGNNEYYEQVLKVLVTLDDPGIK
                     AKRLDENQRWYEIDDIQDLNIAESIFAPEKERTHLIQKRFGGYWRYPKMLDFCYLVNS
                     YYPPQKMMDEIKHNFDVLARQYPSGMAVNSLLAARNFSLRQDQIVVGNGAAELIKALI
                     ERVKGNLGVIRPAFEEYANRCDKEKVIAFVPQTEDFSYNENDVMNFFEDKNLDCLVLV
                     NPDNPTGNYISREGMLKLARWTKKKGIRFLADESFVDFAEETEPTLLAEELLDENPHM
                     IVVKSISKSYGIPGLRLGVAASGDTDLIGQLKKDVAIWNINSFGEFYMQIEEKYRKDY
                     ASALDKLRTERARFAEKLGSLPGVRIFPSQANYLMVELTGENVKATEITELLLTRYNL
                     FIKDLSSKISRDDRQFLRIAIRNEEEDDVLVKALEEILTH"
     gene            complement(6365..7594)
                     /locus_tag="ACZPCL_01085"
     CDS             complement(6365..7594)
                     /locus_tag="ACZPCL_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1161117.1"
                     /translation="MRSKWDIREILQKNAVTVMFVVLCIIGLICSGQTVSYVMYELFG
                     RLSRNAFIVLALIIPIVAGMGINFAITIGAMAAQIAALWVIEWGISGLPGFLVAMLMT
                     MPIAAFFGFLIGKLMNKMKGQEMIGGLILGYFANGLYQLLFLFIFGNLIPLKAPGLVI
                     KGSTGVANTIDLSTDRGFKYALDGLLRVSFSTAVLIICGIIAVGAMVLFARKKMEKKK
                     AFTYGGICVAAIVVAQLPFVKNIFSMVTVPMVTFFVVGLLCIFNNALMKTKIGQQFRA
                     VGQNRTVANASGINVDRVRVIAIVISTVLAGWGQLIFVQNMGSFQTYGAHEQVGLYAG
                     AAILVGGASIKKATNGQALLGCILFHLLFIVAPSAGKNLFGDAAIGEYFRVFISYGVI
                     ALALVMYAWGDYKKKKN"
     gene            complement(7587..9452)
                     /locus_tag="ACZPCL_01090"
     CDS             complement(7587..9452)
                     /locus_tag="ACZPCL_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1161118.1"
                     /translation="MTKNKIQRLLAILCAVAAAFCMVLGFINMPKQNGAAGQEILRTM
                     RIQTLLSATGDSVVESYVAIAKQEAQAAAKASGGGMAAIREAVEKAETETRAKYENGA
                     QSDLSGIDVTGLSAAVDVYTEAVKAYAEVEAQARAAYEEAHYAEAEAALEKKHEEMSA
                     AGEEIPEDDEVVVDMSGFEATPEMLEKQEEAKATYANVGAELKKIYPVLTDEALETLE
                     ATVEGILYQSGDSFATQYDRYVEQSNIKETNEGRIIRHADDLVYLGFALLVLTVLLLF
                     NSWLVAKLGIPRVIIGVFFILLCFMTLWYDLSLSTLLSNTVVRMGMNAIMVLAMVPGI
                     QCGISLNLGLPIGLVAGLIGGLLTIEFGIPGWGGFLFAIVAGSALAAVCGYLYALMLN
                     RLKGSEMSVTTYVGFSIVSLMCIAWLVLPFKSLKLRWPLGTGLRNTIGLDGTNFRHIL
                     NDFLAFQIGDFTIPTGLLLFMALSCFIVWMFSRSCTGQAMQAVGNNPRFAESVGINVD
                     KMRITGTVFSTVLGAVGILVYSQSYGFMQLYTAPRQMGFIAASAILIGGASTSRCKIS
                     HVLIGTFLFQGVLTLGMPVANVLVPGSTISETLRILISNGIILYALTKSGGESRA"
     gene            complement(9449..11065)
                     /locus_tag="ACZPCL_01095"
     CDS             complement(9449..11065)
                     /locus_tag="ACZPCL_01095"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1161119.1"
                     /translation="MNTEKEYVLEMKDITKHYGENTVLNGVSLSVKKGEIHALLGENG
                     AGKSTLMNILFGMPVIHSTGGFEGKVLLDGEETHIHSPYDAMEKGIGMVHQEFMLLPG
                     FTITENIKLNREITNPNPVSRLLGKNLESLDMKAMAFDARKALDDVGMNLDEKTLVHG
                     MPVGYMQFIEIAREIDKTGVKLLVFDEPTAVLTESEAAQLISVMKKISESGIAIIFIT
                     HRLDEVMAASNNITILRDGKLAATRETAKTSIREIAELMVGRKGEAMVEAAPRPVPSE
                     KVAMSIHDLHVDMPGEQVNSLSVDIYEGEIFGIGGLAGQGKLGIPNGIMGLYDAEGKV
                     ELFGQELNFKDTRSVLSAGVAMVSEDRKGVGLLLDRSIEDNIVFNAMQIKGEYLKKFG
                     PFTQIDSKKIRETAEEYIKSLDIRCFGPTQHSGTLSGGNQQKVCIAKALCMSPKFLFV
                     SEPTRGIDIGAKKLVLETLVRLNRELGMTVVIVSSELQELKSVSDRIAIVSEGKLVDI
                     LSPQAPDADYGLAMSGIKPSEHIEKGGTEE"
     gene            complement(11155..12393)
                     /locus_tag="ACZPCL_01100"
     CDS             complement(11155..12393)
                     /locus_tag="ACZPCL_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024095.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HIR1161120.1"
                     /translation="MKSKKMMALVTAAAMAFGLAGCGGGNTAATTAAPAEKAEEKTTA
                     AEAVTEATEAAKSEGSDNWKVAILTGTVSQGEEEFRAAEKALATYGPEHIVTATYPDN
                     FMSEMETTVSQIVSFASDPDVKAIVMCQAVPGAKAGFDKVREMGRDDILLIAGTPQED
                     PAVIAAASDLVLYADEAAQGDTIMEKCADWGIDVFVHYSFPRHMAMELIVARHELLKK
                     NADALGIEMVDVTAPDPTAEAGLSASQQFILEDVPQQLKKYEGKKVAFFTTNCGMQPS
                     LQTACLDEPNAYYPQPCCPSPYHAFPATLGLELAIGGDDEDALKQIAAKLKEHDAVGR
                     FSTWASPVAMTIIAVGVDYAKGYITGEITDRNDADKLVEMFNSKVEGAKINNYTNAEG
                     ETFDNYYTILLAPVDFNDYL"
     gene            complement(12874..13980)
                     /gene="glf"
                     /locus_tag="ACZPCL_01105"
     CDS             complement(12874..13980)
                     /gene="glf"
                     /locus_tag="ACZPCL_01105"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HIR1161121.1"
                     /translation="MKKYDYILVGSGLYSGVFAWFAKQKGKKCLVLEKRDHIGGNVYC
                     ENTEGIHVHKYGAHIFHTSNKEVWQFVNSLAEFNRYTNSPVANYKGEMYNMPFNMNTF
                     SKMWNISTPAEAKKIIEEQKKEITGEPKNLEEQAISLVGREIYEKLVKGYTEKQWGRD
                     CTALPAFIIKRLPVRYTYDNNYFNDLYQGIPIGGYNVIIDRLFEGCDIETGVDYLEKK
                     EYYDGLGEKIVYTGTIDAYYKYQFGKLEYRSLRFESEVLDEENHQGVAVVNYTDRETP
                     YTRIIEHKHFEFGTQPKTVITREYPVTWQEGMEPYYPVNDEKNQALYQKYAKLAEKEE
                     HVIFGGRLGEYKYYDMDKVIASAMACAKEELNML"
     gene            complement(14081..14905)
                     /locus_tag="ACZPCL_01110"
     CDS             complement(14081..14905)
                     /locus_tag="ACZPCL_01110"
                     /inference="COORDINATES: protein motif:HMM:NF017655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HIR1161122.1"
                     /translation="MKNKMEYAYAGGASADTGSTFGLSGAALKWIAMISMLIDHIGAV
                     VVEYGLYYQGGAECFQKLLENGNGQWLYVLQRGMRMLGRPAFLIYAFLLVEGFYHTHS
                     RRRYALQMFLFALISELPFDLAAANRWFYPGYQNVFFEFLIGLAVLWGMEQAQFREKT
                     PLAVEAGKIFAVALGCMAAVLIQSDYDYIGIIIIAAFYEFHYSRAGRSVAAAVLGGLE
                     TLDLNYGVGALAAIPIWFYNGKRGKQKFKYVFYWFYPVHLTVLVLIRAGFLGIFPW"
     gene            complement(14895..15707)
                     /locus_tag="ACZPCL_01115"
     CDS             complement(14895..15707)
                     /locus_tag="ACZPCL_01115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1161123.1"
                     /translation="MKEYKIIFSDIDGTLLNNENQVPEETIEELRRLDSQGVPFVLVS
                     ARMPKGMRPIREELGIHAPMVCYSGGLVLDPDDQEIYSCLLDLDLACEIKSLVAKEFP
                     EVVCGIYGGEHWVVDDIRDPWVIQEQGITHLIPETGDIREIFGKEGGIHKFLFMGDEE
                     AVIRTENRLKERYPQLSIARSKPVYLEVMNGNVCKSAGVKIVCEKYDIPVEQSLAFGD
                     GQNDLDMLNAAGAGFAMANAPEEVKAAAPFVTPVDNEHQGLLAVLRKYFDEK"
     gene            complement(15758..16351)
                     /locus_tag="ACZPCL_01120"
     CDS             complement(15758..16351)
                     /locus_tag="ACZPCL_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR1161124.1"
                     /translation="MHELKVARSQALTTREMAQIGLFTALIAAGAYIKINIPVQPFPM
                     HFTMQFLFVLLAGFLLGARRGAICVGIYLAVGLSGIPVFAAGGGPAYLIRPTFGFLLG
                     FVFAAWITGKLSSLRPGAGTAWDLFSAFWGMMAYYVSGMVYFYGISNYVIHMPISWKL
                     VVINCFLITVVEDFLLCVMAAFLAKRIRALAGDMFQN"
     gene            complement(16567..18234)
                     /gene="leuA"
                     /locus_tag="ACZPCL_01125"
     CDS             complement(16567..18234)
                     /gene="leuA"
                     /locus_tag="ACZPCL_01125"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR1161125.1"
                     /translation="MLNYKRYKRVPVVNFPERTWPNKEIEKAPVWCSVDLRDGNQALV
                     EPMVVEEKVEMFNLLVKLGFKEIEVGFPAASQIEYDFLRTLVDRKLIPDDVEVQVLVQ
                     CREHLIKRTFEALKGIKKAIVHIYNSTSTLQRDVVFHKDMDEIKDIAIVGTKMVQRYA
                     AECDTKIRLEYSPESFTGTELDFALDICTAVQETWGATKENPIIINLPSTVEMNTPNV
                     YADQIEWMNTHFKNRESIILSVHPHNDRGEGVASTELALLAGADRVEGTLFGNGERTG
                     NVDILTVAYNMFSQGINPELNIENVREIAEVYERCTKMQIPPRHPYAGKLVFTAFSGS
                     HQDAINKGMQALHERNSQYWEVPYLPIDPSDIGRQYEPIVRINSQSGKGGVAFVMDTF
                     YGFKLPKGMHKEFADVIQAISEKQGEVAPEQIMEEFKKDYLDRKEPMHFRKCRITDTE
                     TADGEFATLVKLTYTDHGIEKYFDGIGNGPIDAVQRGLEEALGIQIKVLDYTEHALAS
                     GSGAQAASYIHLVDQSTGKATYGVGISSNITRASIRGIFSAVNRLFY"
     gene            complement(18656..20008)
                     /gene="glmM"
                     /locus_tag="ACZPCL_01130"
     CDS             complement(18656..20008)
                     /gene="glmM"
                     /locus_tag="ACZPCL_01130"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR1161126.1"
                     /translation="MGKYFGTDGFRGEANVNLTVEHAFKVGRFLGWYYGQKKPDERCR
                     IVIGKDTRRSSYMFEYSLVAGLTASGADVFLLHVTTTPSVSYVVRTEGFNCGIMISAS
                     HNPYYDNGIKVINEKGEKLEESVIVEIEKYLDGEMGEIPLAKKDKIGRTVDFAAGRNR
                     YIGYLISIATRSFKNMKVALDCANGSASAIAKNVFDALGAETHVISNEPNGLNINTDC
                     GSTHIEHLQKFVVEEGCDVGFAYDGDADRCIAVDEKGNVVDGDGIMYVCGKYMKEQGS
                     LFHNTVVTTIMSNFGLYKAFDREGISYEKTAVGDKYVYENMAATGNCLGGEQSGHIIF
                     SKHATTGDGILTSLKVMEVILEKKQTLAKLASEYEVYPQVLKNVKVKDKKAAQDDEKV
                     QAEVAKVTESLGSNGRILLRQSGTEPVVRVMVEASDLETCEKYVDQVIKVMEEQGHCL
                     "
     gene            complement(20088..20996)
                     /gene="rfbD"
                     /locus_tag="ACZPCL_01135"
     CDS             complement(20088..20996)
                     /gene="rfbD"
                     /locus_tag="ACZPCL_01135"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR1161127.1"
                     /translation="MVRCTLRVHNKSEGSFMRVLVTGVKGQLGHDVMDELAAKGIEGI
                     GVDVEEMDITDAAACEKVITEAKPDAVIHCAAYTAVDAAEDNVELCRKVNAEGTRNIA
                     KVCKALDIKMMYISTDYVFNGEGLRPWEPDDHREPLNVYGLTKYEGEIAVEQNLEKYF
                     IVRIAWVFGVNGKNFIKTMLRLGQERGAVSVVNDQIGSPTYTYDLSKLLVAMIQTDKY
                     GRYHATNEGLCSWYEFACEIFRQAGMDEVKVTPVESSAFPVKAKRPSNSRLNKDKLTE
                     NGFERLPAWQDALGRYLEELKKNDMF"
     gene            complement(21057..22079)
                     /gene="rfbB"
                     /locus_tag="ACZPCL_01140"
     CDS             complement(21057..22079)
                     /gene="rfbB"
                     /locus_tag="ACZPCL_01140"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR1161128.1"
                     /translation="MKIIVTGGAGFIGSNFVHHMVNKYPDYEIINLDLLTYAGNLENL
                     KPVENKPNYRFVKGDIADRKFIFDLFEKEKPDVVVNFAAESHVDRSITDPEAFVRTNV
                     MGTTTLLDACRTYGIKRYHQVSTDEVYGDLPLDRPDLFFTETTPLHTSSPYSSSKASA
                     DLFVLAYHRTYGMPVTVSRCSNNYGPYHFPEKLIPLIISRALADEELPVYGTGENVRD
                     WLHVSDHCEAIDLIIHKGRVGEVYNIGGHNERTNLEVVKTILKALNKPESLIKFVTDR
                     PGHDRRYAIDPTKIETELGWKPKYNFDTGIQQTIQWYLDNQEWWKNILSGEYSNYFDK
                     MYGNRL"
     gene            complement(22254..23153)
                     /gene="rfbA"
                     /locus_tag="ACZPCL_01145"
     CDS             complement(22254..23153)
                     /gene="rfbA"
                     /locus_tag="ACZPCL_01145"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIR1161129.1"
                     /translation="MKGIILAGGSGTRLYPLTKVTSKQLLPIYDKPMIYYPLSVLMNA
                     GIRDILIISTPVDTPRFEALLGDGKQFGINLSYAVQPSPDGLAQAFIIGEEFINGDSV
                     AMVLGDNIFHGQGLNKRLKAAANKGKGATVFGYYVDDPERFGIVEFDANGKAISIEEK
                     PEKPKSNYCVTGLYFYDNRVVEYAKNLKPSARGELEITDLNRIYLENGDLDVILLGQG
                     FTWLDTGTHESLVEATNFVKTVETHQHRKIACLEEIAYLNGWITKEQVLETYEVLKKN
                     QYGKYLKDVLDGKYVDKLHNREF"
     gene            23400..25139
                     /locus_tag="ACZPCL_01150"
     CDS             23400..25139
                     /locus_tag="ACZPCL_01150"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HIR1161130.1"
                     /translation="MLLMNKTLLKLARGLWLWILAIAGVGFLTLVGTTALAEIIAGFL
                     GTLFQPQAILNTAWSAIRAALAAAVFTFLAQLLKGLLEYRTAAKAREGMRKMIFSKVL
                     ELDAGGIEKIGPVSAITASVDAVEQMQTYFSTYLPSLIYSVIAPIYLFFHLKNISMPV
                     AVLLLVVSLFLLPINNAFRSRIEDIRKIYWRSLDDMTGYYMDSLRGLTTLKLFDRDRE
                     HSRILGEKADILNKNINCFMKINFTSFLVTEALIYAALVIALVDSCVRITKGSITISQ
                     ALIVLMLSYSYFSSAQQLMSASHSALTAISAAGKVEEILDTDTTRPFDPSLSEDKEHF
                     NGIRMEHVSYGYEGRARALQDVSLTIPKGSVVALVGLSGCGKSTTASLLMRFCDAAAG
                     HIYMDGKDYHSMKPEELRKHIAMVPQQVNLFSGTIRENLLLADPDADDQTLMEAVKEA
                     GLLKFVQSLEKGLNSDVGNAGASLSGGQRQKIGIARALLSKSEYMIFDEATSSVDPES
                     EKEIWETIGHLAHTRTLIIISHRMSSVRGADCIYVLKDGRVAQQGDHETLMAQDGLYH
                     ELVIRQQAMEVAE"
     gene            25136..26800
                     /gene="cydC"
                     /locus_tag="ACZPCL_01155"
     CDS             25136..26800
                     /gene="cydC"
                     /locus_tag="ACZPCL_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0050456 - L-cystine reductase (NADH)
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HIR1161131.1"
                     /translation="MKRKFSYSMKKMNRRLLAIGRPIRNYIAISTVASVLGALSHMGL
                     MGFGALWLLAAAGFCDGMMTYAALTVTCAVLIAVCRYLEGLFSHLGAYGILAKMRVHL
                     FDAIDRISPAYMIGRETGDIMNIAVADIETLEYFFAHTIGPMFTVILLPVTTVVLAWF
                     VNPLYALVLIPIYIMISVVLPLGALIAGRGIGMRYREELGDLKSKILESVYSIRDIQI
                     FGAGNRKMEDVMKTNSQVNKAALGLTLHRQTIASFPNFFIYLARILILVCAGYLASKG
                     IDNPVGTIAISFAATASLSSSFSLTFVVTSLLEAYGAAERIFKIEDTLPETEEPEHAV
                     TCGEIQTIEFKDVTFAYPGTERKILEHFNYVIHKGDQIGIAGESGAGKSTLLRLLLRF
                     YAPTEGQILINGIPLEQISFRELHKRIAFLEQDTYLFDMTIAENIGIAKPGASMEEIK
                     DAAKRAGIAEFIHTLPDGYDTDMGQMSARLSGGERQRIGIARILLRNPDICLMDEPSS
                     ALDALHEKELLHTLQTAYAGKTLLLISHRGSTLTGCSRILRAEDLK"
     gene            complement(26945..27307)
                     /locus_tag="ACZPCL_01160"
     CDS             complement(26945..27307)
                     /locus_tag="ACZPCL_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR1161132.1"
                     /translation="MVNAIVILILILIAIFAIRGTIKHMKGESPCCGGGGASEIKEKP
                     SKLERPVIGEKIVQISGMTCEHCVNRVMRALNKIDGVSANVSLKGKRAVVSYDREIDV
                     NVLKKAVEDAGYTVESIS"
     gene            complement(27347..27493)
                     /locus_tag="ACZPCL_01165"
     CDS             complement(27347..27493)
                     /locus_tag="ACZPCL_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161133.1"
                     /translation="MGNSSGSMLYMVVLLIIIYVVGMGIYQGVKFIQKKREEAKDKKF
                     REEE"
     gene            complement(27568..27786)
                     /locus_tag="ACZPCL_01170"
     CDS             complement(27568..27786)
                     /locus_tag="ACZPCL_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161134.1"
                     /translation="MIANVIILLLVFGYCGLVIYWKRTGVLKDSCSSCGSGNGGCGGG
                     CGGCSGHCSSCGSAHAHHMDSHKAAKKH"
     gene            complement(27946..30129)
                     /gene="feoB"
                     /locus_tag="ACZPCL_01175"
     CDS             complement(27946..30129)
                     /gene="feoB"
                     /locus_tag="ACZPCL_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR1161135.1"
                     /translation="MAIKIALAGNPNCGKTTMFNALTGANQYVGNWPGVTVEKKEGKL
                     KSKSQSEEVIVTDLPGIYSLSPYTLEEVVSRDYLLKDDPDVIVNLVDATNIERNLYLT
                     TQMIETGIPVVIALNMADLLAKRGIKIDIERLSMLLDCPIVETSALKQTGLDKLIDEA
                     VKVAKKKEADLPKEIFSKELEAAVAEVEGVLPADITENKKRWYAVKFLENDSKVVEST
                     PLSASGKSIVENARKAIEEAHDDDMESIVTDERYKFIQKVVGTAVKKSGEKLTVSDKI
                     DQIVTNRFLGIPIFMLVMWIVYYISVTTIGTFVTDWTNDTFVGAIQEVVGGFLENAGA
                     SDLILSLVVDGIIGGLGAVLGFVPQMAILFLFLSILEDCGYMVRIAFVMDRVFRHFGL
                     SGKSFIPLLISSGCGIPGIMASKTIEQDNDRRLTIMTATFIPCGAKLPVIALMGGVMA
                     GYVTGWDVAGMMAPIMYFVGIVAVLVAAIMLKKTKPFSGKPAPFVMELPQYHIPSAKT
                     VLMHVWERLKGFIIKAGTILFLACVVMWLLSTFGFADGSFGIVEDSADSLMAIIGGAI
                     APLFAPLGWGEWQPVAAAISGFSAKEGIVSTMGVLANVAGDVEDAETVAEGVGMWFPT
                     ALAGFSFLLFNLLDSPCLAAISTMAQQMQDRKWFWFAILFQNVFAYAVTLCVYQIGSF
                     LFYGSFGAGTVVAFVVLLFMLYMLFRPDPYKDQKTYSKRSVQSAS"
     gene            complement(30197..30415)
                     /locus_tag="ACZPCL_01180"
     CDS             complement(30197..30415)
                     /locus_tag="ACZPCL_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1161136.1"
                     /translation="MTLGDAAVGSTVVVTKITGDGAYKRRIMDMGITKGSELYIRKVA
                     PLGDPVEITVRGYELSVRKNDAQCVEVK"
     gene            complement(30485..30694)
                     /locus_tag="ACZPCL_01185"
     CDS             complement(30485..30694)
                     /locus_tag="ACZPCL_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1161137.1"
                     /translation="MPLTMASIGEANTIRKVGGNEETKRFLENLGFVAGAEITVVSAI
                     GGNVIVNIKDSRVAINQDMARHIMV"
     gene            30984..31898
                     /locus_tag="ACZPCL_01190"
     CDS             30984..31898
                     /locus_tag="ACZPCL_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1161138.1"
                     /translation="MTLQQMRYFIGAAESGSISEAAKRSFTAQSSISGAIKEVENTYG
                     ITAFVRDSKGVRLTRSGEELLIEFKWILNRLDYLDEKFNGSKNRPLGLSVSSQHHICG
                     MNSFVSIINSLNTGAGPYHCGFHECRTSEVMDRVERGLDDLGIVFVTEYSKGQMMQEL
                     RNRGIIFNHITYQTSHVYICQNHPLAGSREIDIEDLIQYPFITYDRSSDTNSAYTDLI
                     VPYHQLNRVIQVSDRAASYSVMRYCQGFAVGSGYRPFDDNYSDIVSIPIKNGRRLEIG
                     WIVRQKYTLSDTASRFVDLLMQIESVVL"
     gene            32030..32845
                     /locus_tag="ACZPCL_01195"
     CDS             32030..32845
                     /locus_tag="ACZPCL_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HIR1161139.1"
                     /translation="MEKLKTVINKYWKVYVVALILATVCDLIGTLKFNIGIGTLTLFP
                     MVFATIFGGLLGPDAFKLFKTEESKLGGSLVLVALAPFMAKMGISAGGNLAKLVAVGP
                     ALLLQEFGNLGTILISLPLALLLGLKKEAIGACYSINRDSNLGLTTDIFGPDAPETRG
                     TFAVYIVGSVIGTVFISILASIVASWHIFDPIALGMACGVGSGSMMTAAAGTLGEIYP
                     QQAESIMIMGGASDMLTGITGIYMGTFVALPLTKKLYAILEPKIGHKNNKEAK"
     gene            32845..33306
                     /locus_tag="ACZPCL_01200"
     CDS             32845..33306
                     /locus_tag="ACZPCL_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161140.1"
                     /translation="MVRKYFLQQLGLLIMVSVIMVLTNILGYKMPVGDSIAGILVLCA
                     IALFGLTVSKFMSQFIKLPSMMYVSLTGLLLACPISPVKDFVIGVTSQVAFMAPTTAL
                     GAFAGISLGKDLKDFAKMGWKLVLITLFVITGTFIFSALVADVILKLTGAI"
     gene            33327..34664
                     /locus_tag="ACZPCL_01205"
     CDS             33327..34664
                     /locus_tag="ACZPCL_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1161141.1"
                     /translation="MEKYDILIKNCQVLNPDMTVSSTCSVGIKDSWIKKIGPTDELMA
                     DSSASEIIDGKGKLVMPGMVDGHTHTCQQLLRGRVADEYPMVWTRFLVPFESNLQPED
                     SYISGLLACLEMIKSGTTAFADSGGVHMDRVADAVIESGMRAAIAKSTMDMGNAITGA
                     MKETANEAIENTKKLYQKYQGAGDGRVDIWFAIRQVMTCSRELIAMVGESAKEFHTGI
                     HAHLCEHKDEVSFCLQNYQKRPAEFLEEMGILGPNLLTAHNVMLSDHDIALMAERGVK
                     MIHCPRANLANHGFPKAPQILEAGASLGLGCDGAAPSNLDIFDEMKVLRYAMMAYWGI
                     PSFNPVVMTCPTLLKMASQGGANALGHGDILGSVEEGKKADLILLNIDQPHITPSQNL
                     VNTIVDAANGHDVTDSIINGKIVMKNREVLTLDEERIRFEAEKHMNDIIKRAY"
     gene            complement(34769..35725)
                     /locus_tag="ACZPCL_01210"
     CDS             complement(34769..35725)
                     /locus_tag="ACZPCL_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR1161142.1"
                     /translation="MKIVVLDGYTENPGDLSWDEMKKYGELTVYDRTLAEDAVKRIGN
                     AEIVFTNKTPITKETMDACPDLKFISVLATGYNVIDVNYAKEKGIVVSNIPAYGTDCV
                     GQFAIALLLEICHRIGHHDKAVKEGRWENAPDWCFWDYPLIELAGKTLGIIGFGRIGQ
                     KTGTIAKAMGMNVLAYDSHPNDKGRAIAEYVELDEIFTKSDVISLHCPLFPSTEGIIN
                     KDNIARMKDGVIIINNSRGQLIVEKDLADALNSGKVYAAGLDVVSTEPIKSDNPLLKA
                     KNCIITPHISWAPKEARQRIMDMSVENLKQFLAGDPVNVVNW"
     gene            complement(35780..36910)
                     /locus_tag="ACZPCL_01215"
     CDS             complement(35780..36910)
                     /locus_tag="ACZPCL_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1161143.1"
                     /translation="MYRILLVDDDQNIVDGLGSIIKQNFENIFVISSACDGSDALKRL
                     TEDYYHVIISDIRMPKLDGISLLELISKNQIPSVVIMLSGYDDYTYIRNALRLGAYDY
                     LLKPVNIQNFVSMLRLLIPRLKDSSVKLQAEEISCVRPETQGDYFDIPAKKPYYTKED
                     LEKEMEHLSAGLLSMDEKGTKDKIQEIFSHLSQEAVTKEQFKQVFSAFLYTLMQKNTA
                     LIPIIAGYKLSDNDLAAQIKNQPHISQLRQKMEQILLLYMEQLKKQQEKKEEYVVKKA
                     QEYMKKHCSEQLLLSDIAAQFHLHPNYFSSLFKKQLDMTVRDYILQLRMTKAKELMKD
                     SSLKLLDIALAVGYEDAAHFNRAFKNVTGVSPSMYRDGIKKQ"
     gene            complement(36885..>38194)
                     /locus_tag="ACZPCL_01220"
     CDS             complement(36885..>38194)
                     /locus_tag="ACZPCL_01220"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1161144.1"
                     /translation="SLCIGRGILDLNTIKIVGYCVAGIDTSYLENYFEQNRQTPNQRF
                     AVYKDGKLLYGNIEQEESYQGFVKENLKKTERKENYQSRKLKHSDKNSCVYNVIGHAN
                     GYTLVLQTPLSDIFGNMGRIQMLNIIPIGMILGGIIFMIFQMVGRILKALNRLIEACN
                     HFELGHINKVSKEGLPEEFQTLVSSFNIMSKRIDLLIHEVFVKQQEKQETELQLLRTQ
                     INPHYLYNTLEIMHMKAYMCQDYETATMAELLGQNLQYGLRNTTKEVPLEEEIHQLNI
                     YRAILAYQYNERIQFNICIDRELYSCHVLKLVFQPIVENAVIHGITDSHQILHIDILG
                     YRDEDRLYIQVSDDGCGMSEEQLEMLRHDIDSPSSNSIGLRNVCRRISLNYGPEYKVS
                     IESAEGMGTMIMLGFPWKDNMSDDMEEERTKKEEQDVPDPISR"
BASE COUNT        10250 a         9142 c         7734 g        11068 t
ORIGIN      
        1 tttaccataa taatcttttt aaaaaaaata atcactaata attcaacatt caacattaaa
       61 tgatcgtaat ccaaacgctc tcctcccgtt ctctcgctgc gcccaaagtt cgattccatc
      121 aaatccattg tcatataccc gctggaacat ttcttccaga ccggcattcc aaagcagagt
      181 cgatgaaaca aatatctgtt tcatatccat gcccctttcc tgttgaactg tttgcattat
      241 acggtctgga atgtaaaatt caaaagcagg gaaatgtaaa atatgagtaa tatttttcat
      301 ttattataat tatgaaattt tatttgcatt tatgatcacc aaatgataaa ataactcata
      361 tagatgatca ttttcagcca ttcagatcaa aaaaggagtt gtattctcag atgcttcagg
      421 aattatatga acaatataaa gatacatttt caaaatcgga ccataaactg atacagtatc
      481 ttctgcgaaa cgaagaacag cttcagtatc tcacttcaga agaactttct gcacacacag
      541 gcatcagtcc tgccacggtt tcaagatttt ggaaaaaaat aggatttcag aatttaaaag
      601 aactgaaaat aaagcagcgg atccaggata ctgctactcc cagttcccgc cttacttctg
      661 cactgaagcg ttttgaagaa accgcttccc ttacagaaga cctgaaaatg cattttggaa
      721 ataatacagc aaaaacgctg gaccgtatgg atccagcact tccagcccgt gcagcagacc
      781 ttttactgca ggcacagcat gtttatatct atgccccgga tgcttcctgc ggactggcat
      841 ccattttttg ttatcgcgca agacgattgg ggatccagcc tgttctctta gaaggtggat
      901 ctgccattta tgaatatatg gtcaacatta ccggcaatga tctggtcctt cttttcagtt
      961 tctcacgtct tttaggtgaa acacggatcc ttttagatca ctgtaacaag ataaactgcc
     1021 cggttatcct ttttacagac ctttttgcga ctcaggaaga aatgccgtcc agactgactt
     1081 tttactgcta tcgcggcgaa ccaaatgaat atcattctat gacggttcct ttactggtcc
     1141 ttgatctgat cattttaaat ctgatgcagg caagcaaaaa cagtctcacc tccagccatt
     1201 atctggagaa actgcgggcc gaatatgcac agatcatacg gagatgacct cagccattga
     1261 caaatcaccg tttttgtgcc tattctatac ttataatgta accataataa atacagatcc
     1321 acaaatgatc ataaggggga gtacaactat gaaaaagcca ttggctgttc ttatcactgc
     1381 tttcagtctt tctttgggac ttacgcctgc atttaccgga atatccggaa atctgattat
     1441 agaggctgag gcacatcaag gccggactga tgcaaacggc ggacatcatg attataaaaa
     1501 caaaagcggt cttggaagct atcactatca ctgcggaggc aatcctgctc atctccatcc
     1561 aaacggggtc tgcccttatg caggcggttc ttctggtaat tccggctcag ctggtggttc
     1621 ttcctccaaa aatccgtctt cttccggaaa tcaaaatcaa acagaaacag cagccgctca
     1681 ggtttctact ggttggcaga aagatgatca tggctggtgg taccgccttt ctgcagatac
     1741 ctactatgct gacggtatag ctgacatcga cggcgccacc tatctattta acagtgacgg
     1801 ctatatgctt accggttggc agcagttaaa tggacactgg tactatttta acgcaagcgg
     1861 tgccatggta actggcggac tgaccataga tgggaaatat tactactttg actctgacgg
     1921 cgtactggac gagacagatt acgatgccgt agatgaacca ggagacgaaa catcagatga
     1981 tgacacagaa tatgactacg attatgatta cgacttagaa gaatattaac aggcaaagca
     2041 caaactgtcg caggttcatc tcaaacagat gttcctacgg cagttttcac tttttacata
     2101 aaagcatata ggattcctct gtacctcttt tgaaattcag tcgaattgac tacgatataa
     2161 gatacttaat agaaatagat tctgccattc aggcaaaacg gctcacatga tatgagccgg
     2221 acttttttat tcatagaatt tataatcatt gatctttatt ttggccatta aaaaccaaac
     2281 tcctgcaata actgttccag atatacagga tccttcagtg cacgctgcag atcatctgca
     2341 cgatccgatg tcagaaggca ggaatataat ttattcagtc tggtagtagc ttctgcacgg
     2401 cctttctgta agccttctgc acgaccttcc tgtaagcctt ctgcacgacc tttctgtaag
     2461 ccttctgcat ggccttctgc caatccttct gcccacgctt cttcccggat ctcatctgca
     2521 tgttcctgaa catactcttt catatactgt tcaaacatca tatagcgttc ccccatcttc
     2581 cggcttcttt tgatctggcg catggactcc tgaagccgtt tgaccaatgc atcttcataa
     2641 tcagcttcac ttccagccag atcagcctta acaaaatcaa gtaatgctac cagttccttt
     2701 ggtatttcct cacgattttt tccatttgta ttaagaaaaa cagtgcagac tccgtcacca
     2761 aatacatttc ccgtttcttc acagtatctg cggatggtat aacggcattt tttctgcccc
     2821 aggggatcaa agttacaaat gaaaatgaca tatgtgtctg ccagctctcc ataacttttc
     2881 cctttttcca gcatttccac atccatctga ctgtggtaat aacggctgcg tttttccaga
     2941 ctgtcttttt tcaccagctg catctcaata tcataatgcg tcctgttttc atcttttgca
     3001 aatacgtcca tgcgaatact cttacattcc ggattgaaaa agaaaccatg ttctgtgctg
     3061 atctcaactc tttcaatctg gatctccaac acacgttcca ggaaacatcg gcagttttct
     3121 gcatccatca taacagctgc aaacataaaa tcgtcatgaa ataccagttc ttccagtgtt
     3181 tttttactca ttttattcct ccatattaca gaggaatcct atactggtag attatcataa
     3241 ttttcctgta catgcaatgg aattcccaag aaaaattatc cccctccatt aaataatggt
     3301 acggttgtac ccccaaaaat ctttcaataa gtttttttga cttttccata taaattgctt
     3361 atgctatact gtagtgagcg tgcaaacaca cacattgaac acggcgcgaa aggagcctat
     3421 cgtgaaattc gcagttacaa tcgttactta caaccgactg gaattattaa aagaatgcat
     3481 acagcaggtt ctgtcccaga cagttccctt ttcttatatt tgtattgtag acaaccacag
     3541 tacagatggt acttcagaat atctggacca gctggcggct ggaactgaaa ccgttttccc
     3601 aaacgccgga aaaccggagt ttcacatcct ccatcttccg gaaaatatcg gcggggccgg
     3661 cggctttgca aagggactgg aagatcttgg aaaaacagac tgtgactgga tcctgatcat
     3721 tgatgatgat gccatgatcg cagcagatta tatagaacag cttcagaaag ccattttaaa
     3781 aacaaactac ctggcttatt ccggcactgt aactactgcc ggagccattg ataccaccca
     3841 cagacgtttc atccagtgtc caagactgat gttttataag ccggtaccgg aagaggaata
     3901 tgccaaagct tcttttgaat acgatatcag caccttctgc ggcctgttgg taaacgctga
     3961 catggtacga aagatcggcc ttccaaaaac agaatatttc atctggttcg atgatacaga
     4021 atactgtctg cggttccatg accagtcacg gatcttaaat gtgactacag ctgttttaaa
     4081 ccacaaaaca gcccccgttg gttccgcccc ggtcatcagc tggaaaaatt actatggttt
     4141 ccgcaatgcc attgacatag gaaagaccta ttccaaaaat ccacacctgt atatggcata
     4201 tatctggctg aaccataaag cccacatttt cttggatacc ttcgggcttc tgcttggaaa
     4261 ccagaaagaa atgcgccgtt accgcatcaa agtctacaaa gatgtcctta aaaaccgcca
     4321 taaaaaacca gacggaatat ccccggatta cctgcctgga agcggataca aatcataaag
     4381 gagaaaaacg catgcaagca atcatcctgg ctgccggaat gggaaaacgt ttaaagcagc
     4441 tgacacagaa taatacaaaa tgtatggtaa aggtaaacgg tgttcccctg atcaaccgca
     4501 ccttaaacca gttagaacag cataacctgt cccggatcgt tattgttact ggatatgaag
     4561 gccaaaaact tcgagaacat attgaaggtc tgcatatcaa aacaccggtt gtctttattg
     4621 acaacccggt ctatgaccgc accaacaata tttactccct tttcctggca aaagactatt
     4681 tattaaaaga agacactctt cttttagagt ctgatatcat ctttgaggac tctgtattaa
     4741 acagccttac tgatgaccca agagacagtc tggcactggc tgccaaatat gagtcatgga
     4801 tggacggaac ctgtctggtc ctctctgacc aggacgatat cgaagccatg atcccaggca
     4861 aaaagttccg tttttccgat accgaacatt attacaagac cgtaaacctt tataaattca
     4921 gccaggaatt ttccaggaca cactatgttc cttttctgga agcttataca aaagcacttg
     4981 gaaataatga atactatgaa caggtattga aggtcctggt aactttagat gatccaggca
     5041 tcaaggcaaa acgtctggat gagaaccagc gctggtatga aattgacgat atccaggatc
     5101 tgaacattgc tgagtccatt ttcgcaccgg aaaaagagcg cactcacctg atccagaagc
     5161 gtttcggcgg ctactggcgc tatccaaaaa tgctggattt ctgctatctg gtaaacagtt
     5221 attatccgcc gcagaaaatg atggatgaga tcaaacataa ttttgatgtc cttgcaagac
     5281 agtatccatc cggaatggct gtaaactctc tcctggccgc cagaaacttc tccctccgcc
     5341 aggatcagat cgtagtagga aatggcgctg cagaactgat caaagccctt atagagcgtg
     5401 taaaaggcaa tctgggcgtg atccgtcctg cctttgaaga atatgcaaac cgctgtgaca
     5461 aagaaaaggt cattgcattc gttcctcaga cagaagattt ctcctataat gaaaatgatg
     5521 tgatgaactt ctttgaagat aagaaccttg actgcctggt attagttaat ccggacaatc
     5581 caacaggcaa ttatatctcc cgtgaaggta tgttaaaact agcccgctgg acaaagaaaa
     5641 aaggcatccg cttccttgca gatgagtctt tcgtagactt cgcagaagaa acagaaccta
     5701 cccttttagc ggaagaactg ttagatgaaa atccacatat gatcgtggta aagagcattt
     5761 ccaagtccta cggtatcccg ggactgcgct taggtgtcgc tgccagcggc gatacggacc
     5821 tgatcggaca gctgaaaaaa gatgttgcca tctggaatat caattccttt ggtgagtttt
     5881 atatgcagat cgaagagaaa tacagaaaag actatgcctc tgctttagac aagcttcgca
     5941 cagagcgggc ccgttttgcg gaaaaacttg gcagtctccc cggtgtccgc atcttcccat
     6001 cccaggcaaa ttacctgatg gtggaactca caggcgaaaa tgtcaaagcc acagagataa
     6061 cagagcttct tttaacccgc tataacctgt ttatcaaaga cctgtcctcc aagatctcca
     6121 gagatgaccg ccagttttta cggatcgcca tccggaatga agaggaagat gatgtgctgg
     6181 taaaagcact tgaagaaatt ttaactcact gatctgaaca ataacaatat aaaaccactt
     6241 ttaatcagct aacaatcagc ggacgcgcca ctggcgcctc ctcgatatgc ttggcaacaa
     6301 aaaagaccgc ccggacctgt tcattatcca gtccagacgg tcttttcgtt gtatttacaa
     6361 cctattaatt ctttttcttc ttatagtctc cccatgcata cataaccagt gccagagcga
     6421 taactccgta ggaaatgaat acacggaagt actcaccgat cgcagcgtca ccaaacaggt
     6481 tctttcctgc ggatggagct acgatgaaaa gtaagtggaa caggatacag ccaagaagcg
     6541 cctgtccgtt ggttgccttt ttaatggatg caccacctac cagaatggct gcacctgcgt
     6601 acagacccac ctgctcatgg gcaccgtaag tctggaagga acccatgttc tgaacgaaga
     6661 tcagctgtcc ccagccagcc agtacggtag agatcacgat agcgatcacg cgaacgcggt
     6721 ccacattgat accggaagca tttgcaacgg tgcggttctg tccaacagca cggaactgct
     6781 ggccgatctt tgttttcatc agggcattgt taaagataca taacagtcct acaacaaaga
     6841 aggtaaccat cggtacagtg accatggaga atatattctt tacaaatgga agctgtgcta
     6901 ctacgatagc agccacgcag atgcctccat aagtaaatgc ttttttcttc tccattttct
     6961 tccttgcaaa caggaccatg gcgcccactg caataatacc gcagataata agaacagctg
     7021 tgctgaagga aactcgcagc agaccatcta aagcatattt aaaaccacgg tctgtagaaa
     7081 ggtcgatggt atttgcaaca ccggtagaac ccttgatcac cagacccgga gcctttaacg
     7141 ggatcaggtt gccgaagata aacaggaaca ataactgata taatccgttt gcaaagtaac
     7201 ctaagatcaa accaccgatc atttcctggc ccttcatctt gttcatcagt ttaccaatga
     7261 ggaaaccgaa gaaagctgcg atgggcatag tcataagcat ggcaaccaga aatcctggaa
     7321 gaccgctgat gccccattcg atcacccata aagcggcgat ctgtgctgcc atggcaccaa
     7381 tggtgatggc aaagttgata cccataccgg caacgatcgg aataataagg gccagtacaa
     7441 taaatgcgtt tcggcttaaa cggccgaata attcatacat tacataagaa actgtctgtc
     7501 cggagcagat gagtccgatg atacataata ccacgaacat gacggtaact gcatttttct
     7561 gcaggatttc acgaatatcc catttactac gcacgagact cacctcctga ctttgtaagt
     7621 gcgtacagaa tgattccatt ggagatcagg atacgcagtg tttcggaaat agtggaacca
     7681 ggtactaata cgtttgcaac cggcattccc agtgttaaaa caccctggaa caggaaggta
     7741 ccgatgagaa catggctgat cttgcaccgg cttgtagaag caccgccgat aaggatagca
     7801 gatgctgcga taaagcccat ctggcggggt gctgtataca gctgcataaa gccgtagctc
     7861 tgggaatata ccagaattcc caccgcgcca agtacggttg aaaatacagt acctgtaata
     7921 cgcatcttgt caacattgat acctacagac tctgcaaaac gaggattgtt tcctactgcc
     7981 tgcatcgcct gaccggtaca gctgcgtgaa aacatccata caataaagca gctaagtgcc
     8041 ataaataaca gcagtcctgt cggaatggta aaatcgccga tctggaatgc caggaaatca
     8101 tttaagatat ggcggaagtt ggttccgtcc agaccaatgg tatttctaag accggttcct
     8161 aatggccagc gaagcttcag ggacttaaac ggcagtacca gccatgcaat acacattaaa
     8221 gatacgatgg aaaaaccaac gtaggtggta acagacattt cagaaccttt taagcggttt
     8281 aacataagtg catacagata accgcagaca gctgccagag cggaacctgc aacaatagcg
     8341 aagaggaaac ctccccatcc tggaatacca aactcaatgg ttaaaagacc accgatcaga
     8401 cctgcaacca gaccaatggg aagacccaga ttcaggctga taccgcactg gataccagga
     8461 accattgcca gaaccatgat ggcattcatt cccattcgta caactgtatt acttaacaga
     8521 gtagaaagag aaagatcata ccataaggtc ataaagcaaa gtaagataaa gaatacaccg
     8581 atgatcactc ttggaatacc aagttttgca accagccagc tgttaaacag taaaagtaca
     8641 gtcaatacta aaagggcaaa gcctaaataa accaggtcat ctgcgtgacg gatgatccgt
     8701 ccttcatttg tctctttaat attactctgt tcaacgtaac ggtcatactg agtggcaaaa
     8761 ctgtcaccgg actgataaag gatgccttct actgtcgctt ccagtgtttc taatgcttca
     8821 tccgtcaata ccggatagat ctttttcagc tctgcgccta catttgcata agttgccttt
     8881 gcttcttcct gcttttcaag catttcagga gttgcctcaa aaccggacat atcaactact
     8941 acttcatcat cttccgggat ctcttctcct gctgctgaca tttcttcatg tttcttttct
     9001 aaagctgcct ctgcctctgc ataatgtgcc tcttcataag cagcgcgggc ctgagcctcc
     9061 acctctgcat aggcctttac agcctctgta tatacgtcaa cagctgcaga aagaccggtt
     9121 acatcaatac cggaaagatc actctgggcg ccattttcgt attttgctct tgtttctgtt
     9181 tctgcctttt ctactgcttc acggatagct gccataccgc cgccggatgc cttggctgcc
     9241 gcctgtgctt cctgtttcgc aatggctacg taactttcca ctacgctgtc gccggtagca
     9301 ctgagcagtg tctggatacg catggtacga aggatctcct gacctgccgc cccattctgc
     9361 ttaggcatat ttataaatcc taataccata cagaaggctg ccgctactgc acaaagaatg
     9421 gcaaggagtc tctggatctt atttttcgtc attcttctgt tccccctttc tctatatgtt
     9481 cggacggttt aatgccggac atggccagac cataatctgc gtctggtgcc tgtggagata
     9541 agatgtctac cagtttgcct tctgaaacaa tggcaatacg atcagaaaca ctcttaagct
     9601 cctgaagttc tgaggaaaca atgacaacgg tcattcccag ttccctgttt aaacgcacca
     9661 gagtctctaa caccaacttt ttcgcgccga tgtcgatacc tcgggtaggt tcggaaacaa
     9721 acaggaattt cggagacatg cacagcgcct tggcaataca taccttctgc tggttaccac
     9781 cggataaagt accggaatgc tgggtcggtc caaagcagcg gatgtccagg ctctttatgt
     9841 attcttctgc ggtttcacgg atctttttgc tgtcgatctg ggtaaatggt ccgaatttct
     9901 tcaaatattc ccctttgatc tgcatggcgt taaatacgat attgtcttca atggaacgat
     9961 ctaataaaag gcccacacct ttacggtctt cagaaaccat ggcaacacct gcgcttaaaa
    10021 cagatctggt atctttaaag tttaattcct gtccgaacag ttctaccttt ccctctgcat
    10081 cataaagtcc catgatacca ttggggatac ccagttttcc ctgacctgcc agaccgccga
    10141 taccgaagat ctcgccctcg taaatatcca cagataagct atttacctgc tcaccgggca
    10201 tatctacatg aagatcatgg atgctcatag ccactttttc acttggaacc ggtcttgggg
    10261 ctgcctctac cattgcttcc ccttttcggc ctaccatcag ttccgcgatc tcacggatac
    10321 tggtttttgc agtttctctt gttgctgcca gctttccgtc tcgcaggatg gtaatgttat
    10381 tagatgcggc cattacctcg tccagacgat gggtaataaa gataatggca ataccgcttt
    10441 cagagatctt cttcataacg gaaataagct gggctgcctc actctccgta agaacagcag
    10501 tgggttcatc aaacaccaga agttttacac ctgttttatc gatctctctt gcgatctcga
    10561 taaactgcat atatccaacc ggcattccat ggaccagcgt tttttcatct aaattcattc
    10621 ctacgtcatc cagagcctta cgggcatcaa aagccatagc cttcatatcc aggctttcca
    10681 ggttctttcc cagaaggcgg cttaccggat tgggatttgt gatctcacga tttaatttga
    10741 tgttttccgt aatagtaaat cccggaagaa gcataaattc ctggtggacc ataccaatgc
    10801 ccttttccat ggcgtcataa ggagaatgga tatgtgtttc ttccccatct aaaaggacct
    10861 ttccctcaaa accgcctgtg ctgtggatga ccggcatacc aaacaggata ttcatcagag
    10921 tagatttacc agctccattt tctcctaaca gtgcatggat ctcgcctttt ttcacggaaa
    10981 gagacacgcc atttaatacg gtattttcac cataatgctt ggtaatgtct ttcatctcaa
    11041 gaacatattc tttttctgtg ttcattgaaa ttcccctttc tgtcagttat cattttgctg
    11101 aaaaaggccc acccgaaatc aatagggtga gccttttctc agatttcaag tttcttataa
    11161 gtagtcgtta aaatcaaccg gagcaagcag aatggtatag taattatcaa aagtttcacc
    11221 ttctgcattg gtataattat tgatctttgc cccttcaact ttagagttaa acatttcaac
    11281 cagcttatca gcatcattgc ggtctgtgat ctcgcctgtg atatatccct ttgcataatc
    11341 aacaccaact gcaataatgg tcatagcaac cggggatgcc catgtagaga aacgtcctac
    11401 tgcgtcatgc tcctttaatt tagctgcgat ctgctttaat gcgtcttcgt catcaccgcc
    11461 aattgcaagt tccagtccta acgtagctgg aaatgcgtga tatggacttg ggcagcaagg
    11521 ctgtggataa taagcattag gctcatctaa gcaagcggtc tgtaaagaag gctgcatacc
    11581 acagtttgtt gtgaagaaag caactttctt gccttcatat ttcttcagct gctgtggaac
    11641 atcttccagg atgaactgct gggaagcgga aagtccagcc tctgcagttg ggtctggagc
    11701 agtaacgtct accatttcga tgccaagcgc atctgcgttc ttcttaagaa gttcgtggcg
    11761 tgctacgatc agctccattg ccatatggcg tgggaaggag taatgtacga atacatcaat
    11821 accccagtca gcgcattttt ccataatggt atcgccctgt gctgcttcat ctgcatataa
    11881 aacaagatca gatgctgctg cgataacagc cgggtcttcc tgaggagtac cagcgatcag
    11941 gaggatgtca tctcttccca tctcgcgaac cttatcaaaa ccagcctttg caccaggaac
    12001 tgcctgacac ataacgatcg cttttacatc aggatcagat gcgaaagata cgatctggga
    12061 tacggtagtc tccatctctg acataaagtt gtccggataa gttgctgtaa cgatgtgctc
    12121 cggaccatat gtagctaatg ctttttctgc tgcacggaac tcttcctcgc cctgtgaaac
    12181 agtaccggtt aaaattgcta ctttccagtt atcactgcct tctgactttg cagcctctgt
    12241 tgcttctgta acagcttctg ctgcggtagt tttttcttct gccttctcag caggtgctgc
    12301 tgttgtagct gcggtatttc caccgccaca tcctgccaga ccaaatgcca tcgctgctgc
    12361 tgtaacaaga gccatcattt ttttgctttt cataattcgt cctccttaat ttatagatgt
    12421 ccagaccatc tacccccctg catgttttca acatggatca tgctgtcctc atggccggcg
    12481 gcatacaagt tttattttgt acgtctacgg tctacacttg tgtgctagtg taacacaccc
    12541 ctgttaaaaa agcaagtttt tttgttgtga atttgtaatt ttttaccatt tatttttgta
    12601 atccatttat atttttatgc tttaaagtca caaattatac aaaaaatctt taatctgtca
    12661 ttttatagct cgtcattttg ctgcattttt cgtctgtaaa tcccctgtat caacagggtt
    12721 tttggaaatt tctggaaaat aatggcgccg gtagttccaa cggaatttcc cggtacacaa
    12781 aaaagctact gcaaaatggt ctctttccac tttgcagcag ctttatctta ctcaggctca
    12841 tatttctgtc cgtcaggcat gacattcccc cgtttacagc atattcagtt cttcctttgc
    12901 acatgccatg gcagaagcga tcaccttgtc catgtcgtaa tacttatatt ctcctaaacg
    12961 tccgccaaaa ataacatgtt cttctttttc agccagtttt gcatacttct gatacagtgc
    13021 ctggtttttc tcatcattga ccggataata aggttccata ccttcctgcc aggtaacagg
    13081 atattctctg gtaataaccg tctttggctg ggtaccgaat tcaaagtgct tgtgctcaat
    13141 gatgcgggtg tacggagtct cgcggtccgt gtagtttact acagcaacgc cctggtggtt
    13201 ttcctcatcc agaacctcag actcaaaacg caggctgcgg tattccagct tgccaaactg
    13261 atacttataa taagcatcaa tggttcctgt atacacgatc ttttccccta aaccatcgta
    13321 atattccttc ttttccagat aatctacgcc ggtttcaata tcgcagcctt caaagagacg
    13381 gtcaatgatc acattgtaac cgccaatagg aattccctgg tacagatcat tgaaatagtt
    13441 attatcatag gtatagcgca caggaagtct cttgatgata aaagccggaa gtgctgtgca
    13501 gtctcttccc cactgttttt ctgtgtagcc ttttaccagt ttttcataga tctcgcggcc
    13561 taccaggctg atggcctgct cttctaagtt cttcggctcg ccggtgatct cttttttctg
    13621 ctcttcaatg atctttttcg cctctgccgg agtggaaata ttccacatct tgctgaaggt
    13681 gttcatgtta aaaggcatgt tgtacatttc acctttgtaa ttggcaaccg gactgttggt
    13741 ataacgatta aattctgcca gtgaatttac aaactgccat acttctttgt tgcttgtatg
    13801 gaaaatgtgg gcaccgtatt tgtgtacatg gataccttct gtattttcac agtacacatt
    13861 tccgccaata tgatccctct tttccaggac caggcacttc tttcccttct gcttcgcaaa
    13921 ccaggcaaag acaccggagt acagtccgct tcctactaat atatagtcat attttttcat
    13981 ttttatgctc cttttttata ccacgacata atccgagtat accgcatctg gcatcatcag
    14041 gcaatcaaca gattttcccc ttatgtgcag actttttata ttaccacgga aaaatcccca
    14101 gaaatccggc tctgatcaac accagaacgg tcagatgcac cggataaaac cagtaaaaca
    14161 catatttaaa cttctgtttt ccccgttttc cattataaaa ccagatgggt attgctgcca
    14221 gggcaccaac tccatagttc agatccagtg tttccaggcc gccaaggact gccgctgcca
    14281 cactgcgccc tgccctgctg taatgaaact cataaaaagc tgcaataatg atgatcccga
    14341 tataatcata atctgactga atcagcactg ctgccataca gcccaaagct actgcaaata
    14401 tctttcccgc ttccactgca aggggagttt tttcccgaaa ctgtgcctgt tccatgcccc
    14461 ataaaacagc cagtcctatg agaaattcaa aaaatacatt ctggtacccc ggataaaacc
    14521 agcggttggc tgccgccagg tcaaaaggca gttctgatat gagggcaaac agaaacatct
    14581 gcagggcgta acgcctccgg ctgtgtgtat ggtaaaagcc ctctaccaga agaaaagcat
    14641 agatcaggaa tgcaggccgt cccagcatac gcattccccg ttgaagcaca tacagccact
    14701 ggccatttcc attttccaga agtttttgaa aacattctgc tccgccctga taatacaacc
    14761 catattccac gaccactgcc ccaatatggt caatgagcat ggagatcatg gcgatccatt
    14821 taagcgctgc gccacttaag ccaaaggtac ttcctgtatc tgcagaagca cctcccgcat
    14881 aggcatactc cattttattt ttcatcaaaa tatttcctca acacagctaa aagcccctga
    14941 tgttcattgt caaccggagt tacgaaagga gccgccgctt ttacttcttc cggcgcattg
    15001 gccatggcaa aaccggcacc tgctgcattt aacatatcaa ggtcattctg cccgtcccca
    15061 aaagccaggg actgctccac aggaatgtca tatttttcac atacaatctt cactcctgct
    15121 gatttacaca cgtttccgtt cattacttcc agataaaccg gcttggatct tgcaatggaa
    15181 agctgtggat accgttcttt taagcggttt tctgtacgga tcaccgcttc ttcatccccc
    15241 ataaacagaa acttatgaat gccaccctct tttccaaaaa tctcccttat atctccggtt
    15301 tccggtatca gatgggtaat tccctgttcc tggatcaccc acggatccct gatatcgtcc
    15361 actacccagt gttccccacc gtatatgccg cagactacct ccggaaactc ttttgcaacc
    15421 agacttttga tctcacatgc caggtccaga tccagcaggc agctgtatat ttcctgatca
    15481 tccggatcca gaaccagacc gccgctgtag cacaccatag gagcatggat ccccagttct
    15541 tcccggatgg ggcgcattcc ttttggcata cgggctgata ccagtacaaa aggaactccc
    15601 tgactgtcca gacggcgcag ttcctctata gtctcctctg gaacctggtt ttcattgttt
    15661 aaaagagtgc cgtcaatatc gctgaaaatg atcttatatt ctttcaagtt gtttaacccc
    15721 cgcatttata atgtaacatt tgagctgaaa ccagtttcta attctggaac atatctcctg
    15781 ccagcgcccg gatccttttt gccagaaatg ccgccataac gcagagaaga aaatcttcca
    15841 ccactgtgat cagaaaacag ttgatcacca ccagtttcca tgagattggc atatggatca
    15901 cataattact gatcccataa aaatacacca ttcctgaaac ataataagcc atcatccccc
    15961 agaaggcact gaacagatcc caggcagtac ctgctcccgg gcgaagggag gacagctttc
    16021 ctgtgatcca ggcagcaaat acaaagccca gtaaaaatcc gaatgttgga cggatcagat
    16081 aagcaggacc gccacctgcc gcaaacaccg gaataccgga aaggcctacc gccagataaa
    16141 tccccacaca gattgcacct ctgcgggctc caagcaaaaa tcctgctaac aggacaaata
    16201 aaaactgcat ggtaaaatgc atgggaaagg gctgcaccgg tatattgatc ttaatatagg
    16261 cccctgctgc gatcagagct gtaaacagac cgatctgcgc catttccctg gttgtcagcg
    16321 cctgagaacg ggccactttt aattcatgca taaatgattt tcctccgtaa tgtatgatat
    16381 cctgaaacat aaaacattgt aacattgacc ataacctaag tcaacgaagg agttcactct
    16441 ggctctgcgt tataacaaaa aaggatattg gcacgtacaa aaaagcgcat tgccataaat
    16501 gacaacacgc tttttgattt cacatttatt tttatttcat accggggatc cggcatttac
    16561 cattcattaa taaaacagtc tgttcacagc actgaagata ccgcggatag atgctctggt
    16621 aatattggag ctgatgccta cgccgtaagt tgcttttcca gtactctggt ctactaagtg
    16681 gatgtaggaa gcagcctgtg cgccggaacc ggatgccagt gcatgctcgg tgtagtctaa
    16741 taccttgatc tggatgccca gtgcctcttc taagcctctc tgaacagcat caatcggtcc
    16801 gtttccaatg ccatcgaagt acttctcaat accatgatca gtgtaggtaa gtttaaccag
    16861 tgttgcaaac tcgccgtctg cagtctctgt atcggtaata cggcacttgc ggaagtgcat
    16921 tggttctttg cggtccagat aatccttctt gaactcttcc atgatctgct ctggagcaac
    16981 ttcaccctgc ttttcggaaa tagcctggat gacatctgca aactccttgt gcatgccctt
    17041 tggaagcttg aagccataga aagtatccat aacaaaggct acgccgccct ttccggactg
    17101 gctgttgata cgaacgattg gctcgtactg tcttccaata tcgcttggat cgataggcag
    17161 atacggaact tcccagtact ggctgtttct ctcatgaaga gcctgcatac ctttgttgat
    17221 cgcatcctga tgagaaccgg agaatgcggt aaataccagc tttccagcgt atggatgtct
    17281 tggcgggatc tgcatcttgg tgcagcgctc gtaaacttct gcgatctcac ggacattttc
    17341 aatatttaac tctgggttga taccctggga gaacatattg taagcaacgg ttaaaatatc
    17401 tacattacct gtacgctcac catttccgaa cagggttccc tctacacggt cagcgcctgc
    17461 aagcagtgct aattcagtag aagcaacacc ttcgcctctg tcattgtgtg gatgcacact
    17521 taagataatg ctttctctgt tcttaaagtg tgtattcatc cattcgatct ggtctgcgta
    17581 tacgtttgga gtgttcatct caacagtaga tggaaggttg atgatgatcg ggttctcctt
    17641 ggtagcgccc caggtttcct gaactgcagt acagatatca agagcaaaat ccagctctgt
    17701 gccggtaaag ctctctggag aatactccag acggatcttg gtatcacatt ctgctgcgta
    17761 tctctgtacc atcttggtac ctacaatagc gatatccttg atctcatcca tatctttgtg
    17821 gaaaacaaca tctctctgaa gagtagatgt ggaattgtaa atatgtacaa tggctttctt
    17881 gatgcccttc aatgcctcaa aagttctctt gatcagatgc tcacggcact gtactaatac
    17941 ctggacttct acatcatccg ggatcagctt gcggtcaacc agggtacgaa gaaaatcata
    18001 ttcgatctgg gaagctgcag ggaaaccaac ctcgatctcc ttaaagccca gctttactaa
    18061 aaggttgaac atctctacct tttcttctac taccattggt tctaccagtg cctggtttcc
    18121 gtcacgaagg tcaacgctgc accatacagg agccttttcg atttccttat ttggccaggt
    18181 acgctccggg aaattaacta caggtactct tttatatctc ttataattta acataataac
    18241 tatttcctcc ttgtgtctat ctctgtcttt tttgttacat gctacttgct gttgtgaccg
    18301 gcatttaaac ggttctgctt tgtatttttt aatctcaagc ccttccgggc agtcgctcga
    18361 tcacgccgta acggcagacg ataaacagca gtacttttac tgattcagcc ccacgtttca
    18421 tcattccttt tcttaataaa atcgtataca gcatgggatt tgtttaaaaa agctccggag
    18481 caacattgtc cccggagcct ctgatacacc agcctcattg ctgcaaatga taaaaaatat
    18541 atattacagt ataacatata ccctttattc ttacaagcaa tgagatttca ttttactatt
    18601 cgttctggtt atggataata ttcctgatca acgtttgcag gcgcattttt tcgccttata
    18661 aacaatgccc ctgctcttcc attactttaa tcacttggtc aacgtatttt tcacaggtct
    18721 caagatcaga tgcctctacc ataacacgga caaccggttc tgtaccagac tgacgaagaa
    18781 ggatacgtcc attgcttcct aagctttctg ttactttggc aacctctgcc tggacttttt
    18841 catcatcctg ggctgctttt ttgtctttta cctttacgtt cttaagaacc tgcggataaa
    18901 cttcatattc agatgccagt tttgcaagag tctgcttctt ctccaggatc acttccatta
    18961 ctttcaggga agtaaggatg ccgtctccgg tcgttgcatg tttactgaaa atgatatggc
    19021 cggactgttc gccgcctaag cagttgccgg tagcagccat attttcataa acatatttat
    19081 cgcctacagc tgtcttttca taggaaatgc cttcccggtc aaatgccttg taaagaccga
    19141 agtttgacat aatggtagta acaactgtgt tgtggaacag gcttccctgc tccttcatgt
    19201 acttgccgca gacatacata atgccgtctc cgtctactac atttcccttt tcatctacag
    19261 cgatgcagcg gtcagcatct ccgtcgtaag caaagcctac atcacagcct tcttctacta
    19321 caaatttctg taaatgttca atgtgggtgg aaccgcagtc tgtgttgatg tttaagccat
    19381 ttggctcatt gctgatcaca tgtgtctcag cgccaagggc atcaaataca tttttagcaa
    19441 tcgcagaagc acttccgttt gcacagtcta aagccacttt catgttcttg aaagaacggg
    19501 tggcaataga gatcagatag ccgatgtaac ggtttctgcc tgctgcaaag tctaccgtgc
    19561 gtccgatctt gtctttctta gctaacggga tctcgcccat ttcgccgtct aagtatttct
    19621 cgatctctac gatcacagat tcttccagct tttcgccctt ttcgttgata accttgatac
    19681 cgttatcata gtatgggttg tggctggcag agatcatgat accgcagtta aagccctctg
    19741 tacgtactac ataagaaacg cttggtgtgg tagttacgtg aagcagaaat acatcagcgc
    19801 cggatgcggt cagacctgct actaaagaat actcaaacat gtagctgctg cgtctggtat
    19861 cttttccgat aacgatacgg catctttcat ctggcttttt ctgtccgtag taccagccta
    19921 agaaacggcc tactttaaag gcatgttcaa cggttaagtt tacatttgct tccccgcgaa
    19981 agccgtctgt tccaaaatat tttcccattt tttatgatcc ttcctatctg cacctgtatc
    20041 tggccgatca ctgcctgatg cagaagtcaa ttttattctg aaccggatta aaacatatcg
    20101 ttcttcttca gttcctctaa atagcgtccc aaagcgtcct gccatgcagg aagtctctca
    20161 aagccgtttt ctgtcagttt atccttattt aagcggctgt tagatggacg ttttgcctta
    20221 acagggaatg cagatgattc taccggtgtt acctttactt catccatacc agcctgacgg
    20281 aagatctcgc aggcaaattc ataccagctg caaagtcctt cattggttgc atgatatctg
    20341 ccgtacttgt cggtctggat catggcaact aacagcttag atagatcgta agtataagtt
    20401 ggggaaccaa tctggtcatt tacaacgctg acagcgcctc tctcttggcc taaacggagc
    20461 atggtcttaa taaagttctt tccatttaca ccaaataccc atgcaatacg gacaataaag
    20521 tacttttcca ggttctgctc tactgcgatc tcgccttcgt acttggtcag accatataca
    20581 tttaatggct ctctgtggtc atccggctcc catgggcgca gaccttcgcc gttaaataca
    20641 tagtctgtac tgatgtacat catcttaatg tctaaggctt tgcagacttt tgcaatgttt
    20701 ctggttcctt ctgcattgac cttgcggcac agttccacat tgtcttcagc agcatctact
    20761 gcagtatatg ctgcacagtg gatcactgca tccggttttg cctcagtaat tactttttcg
    20821 caggctgcgg cgtctgtgat atccatttct tctacatcta cgccaattcc ttcgatgccc
    20881 ttagctgcca gttcgtccat tacgtcatgt ccaagctgtc cttttacacc tgttaccaga
    20941 actctcataa atgatccctc acttttatta tgtactctga gagtacatct tactatacac
    21001 gtcaatggct ggcggaaatt tccgtcagcc acagttactt gttacttatc gttcacttac
    21061 agacggtttc cgtacatttt atcgaagtaa ttgctgtact caccgcttaa aatatttttc
    21121 caccattcct ggttgtccag ataccactgg atggtctgct gaattcctgt atcaaagttg
    21181 tattttggct tccagcctaa ttctgtctcg atcttggttg ggtcgatggc ataacggcgg
    21241 tcatggcccg ggcggtctgt tacaaacttg atcaggcttt ccggcttgtt taatgccttt
    21301 aagatagtct ttacaacttc cagattggtt ctctcgttgt gtccgccaat gttgtaaact
    21361 tcacctacac ggcccttgtg gatgatcagg tcgattgctt cgcagtgatc ggatacatga
    21421 agccagtcac gtacattttc accggttccg tatacaggaa gctcctcatc tgctaatgca
    21481 cggctgatga tcagcgggat cagcttctca gggaaatgat atgggccata gttgttggag
    21541 cagcgggata cagttacagg cattccatag gttctgtgat atgccagtac aaacaggtct
    21601 gcacttgcct tggatgaaga atatgggctg gaggtgtgca gtggtgttgt ctctgtaaag
    21661 aacaggtctg ggcggtctaa tggcagatct ccataaacct catcggtaga tacctgatga
    21721 taacgcttaa tgccataggt acggcatgca tccagtaagg tagtggttcc cattacattg
    21781 gtgcgcacaa atgcctccgg atcggtgatg gaacggtcta catggctttc tgcagcgaag
    21841 tttactacta cgtctggttt ttctttctca aataaatcga agatgaactt tctgtcagcg
    21901 atatcgcctt ttacaaagcg gtagtttggt ttattctcta ctggctttaa gttttccagg
    21961 tttcctgcat aggttaacag atccaggttg atgatctcat agtctgggta tttgtttacc
    22021 atatgatgta caaagttgct tccgataaat ccggcgccgc cggttacgat gatcttcata
    22081 atgtaaatgt ttcctcctaa aatagctcta tgcgagggac atcttcttgc tgcggcaagc
    22141 cgcaacaaga agcatcggat atccatccga tgtaaaattc aagccataag cggacttatg
    22201 agcaagctca acatccgctt atttcttgaa ttttgcgccc tatagcttgt tcgttagaat
    22261 tcgcggttat gcagcttgtc tacatacttt ccgtctaaaa cgtctttcag gtacttgccg
    22321 tactggttct tctttaaaac ttcgtaggtc tctaatacct gctctttggt gatccagccg
    22381 tttaagtatg cgatctcttc caggcatgcg atctttctgt gctgatgggt ctctaccgtc
    22441 tttacgaagt tggttgcttc tactaaggac tcatgggttc ctgtatccag ccaggtaaag
    22501 ccctgtccca gtaagataac atccagatcg ccattttcca gatagatacg gttcaggtct
    22561 gtgatctcta actcgcctct tgcagatggc tttaagttct ttgcatactc tacaacgcgg
    22621 ttatcataga aataaagtcc tgttacgcag taattggact tcggcttttc aggcttctct
    22681 tcaatagaga tcgctttgcc gtttgcgtca aattcaacga taccgaaacg ctccggatcg
    22741 tctacatagt aaccgaatac agttgcgcct tttcccttat tggcagcagc ttttaatctc
    22801 ttgtttaagc cctgtccatg gaaaatatta tcacctaata ccatggcaac ggagtcacca
    22861 ttgataaact cctcgccaat gataaatgcc tgagccagtc cgtccgggct tggctgtaca
    22921 gcatagctta agttgatacc gaactgcttt ccatcaccta acagagcctc aaaacgtggg
    22981 gtgtcaactg gtgtggagat gattaagata tccctgatac ctgcattcat caacacagat
    23041 aacgggtaat agatcatagg tttgtcataa ataggaagca gctgcttcga tgttactttg
    23101 gttaaagggt aaagtctggt gccgcttccg ccggcaagta taatcccttt cattttttcc
    23161 tccttgcagg tccctgtaac cgtattgctt cttattttaa tgcagactac ggccgaacca
    23221 ctggttattc tttgttcaat ataccatact atgcgccatt tttcaaatcc tcagggaaat
    23281 atttcgctat aattcatgaa atattaatat acgatttttt tcaatagggg attagggatt
    23341 gaaaaaagtc tttttattta ataagatagc agtaaaacat acaaaattgg gggaaaacta
    23401 tgcttttaat gaacaaaact cttttaaagc ttgcccgggg actatggctt tggatcttag
    23461 ccatagcagg ggtgggcttt cttacgttgg taggtaccac ggcgctggct gagatcattg
    23521 ccggtttcct tggtactctt tttcagcctc aggcgatctt aaatactgcc tggagcgcca
    23581 tccgggccgc gctggctgca gctgtattta cctttcttgc ccagctctta aaaggactgt
    23641 tagaatacag gaccgctgca aaggcaagag aaggcatgcg gaaaatgatc ttttccaaag
    23701 ttctggaatt agacgcagga ggcatcgaaa agatcggtcc tgtctctgct atcacagcct
    23761 ctgtagatgc cgtggaacag atgcagacct atttcagtac ctaccttcca agtctgatct
    23821 acagtgtgat cgcgcccatt tatctgttct ttcatttaaa gaacatttcc atgcctgtgg
    23881 ctgttttatt attagtagta tcactttttc tgcttcctat taataatgca ttccgcagca
    23941 ggatcgaaga tatccgcaaa atctactggc gttcactgga tgatatgacc ggctactata
    24001 tggacagcct gcggggactt actactttaa aactgtttga cagagacagg gaacattccc
    24061 gtatcctggg ggaaaaggca gatatattaa acaaaaatat caactgtttt atgaagatca
    24121 attttacctc tttcctggta acagaagcgc tgatctatgc tgccctggta atcgcccttg
    24181 tagactcctg tgtacgtatt acaaagggtt ccattaccat ttcacaggca ctgatcgttt
    24241 taatgctttc ttacagctat ttttcttctg cacagcagct gatgagcgct tcccacagtg
    24301 ctttaaccgc tatttccgca gcaggtaaag tggaggagat ccttgataca gatacaacac
    24361 gcccctttga cccgtcactg tcggaagata aggaacattt taatgggatc cgtatggaac
    24421 atgtatccta tggttatgaa ggacgggcca gagccttaca ggatgtttct cttacaatcc
    24481 ccaaaggttc tgttgtcgct ttagttggac tttcgggctg tggaaaatcc actactgctt
    24541 ctcttttaat gcgtttttgt gacgcggcag ccggacatat ttacatggac ggcaaagatt
    24601 atcacagtat gaaaccggag gaactgagaa agcacattgc catggttccc cagcaggtaa
    24661 acctgttttc cgggactatc cgggaaaatc tgctccttgc tgacccggat gcagatgacc
    24721 agactttaat ggaggctgta aaagaagcgg gacttttaaa atttgtacag tcactggaaa
    24781 aaggactgaa ttctgatgtt ggaaatgcag gtgcttccct ctccggcgga cagcgccaga
    24841 agatcggtat tgcaagagcc cttttatcaa agtctgaata catgatcttt gatgaggcta
    24901 cttccagcgt tgatccggaa agtgaaaaag agatctggga gaccatcggc cacctggccc
    24961 atacccggac ccttatcatc atctcccata gaatgtcatc ggtacgtggc gctgactgca
    25021 tttacgttct aaaagacggc agggtggcac agcagggcga tcatgaaact ttaatggccc
    25081 aggatggctt atatcatgaa ctggtaatac gccagcaggc aatggaggtg gcagaatgaa
    25141 acgaaaattc agttattcaa tgaagaaaat gaaccgccgg cttctggcca ttggaagacc
    25201 gatccggaac tatattgcca tttcaactgt tgccagtgta ctgggagcac tctcccatat
    25261 gggactgatg ggatttggcg ctttatggct tttggctgcg gctggttttt gtgatggaat
    25321 gatgacttat gcagccctta ctgttacctg tgctgttttg atcgcagtat gccgctatct
    25381 ggaaggttta ttctcccatt taggtgcata cggcatcctt gcgaaaatga gagtccatct
    25441 ttttgacgcc atcgaccgta tttctcctgc ttatatgatc ggacgggaaa ccggtgatat
    25501 tatgaatatc gctgtggcag atattgaaac tcttgaatat ttctttgcac atactatcgg
    25561 acctatgttt acagtcattt tactgccggt aacaacagtg gttctggcat ggtttgtaaa
    25621 tccgctgtat gcactggtgc tgatcccaat ctatattatg atcagcgttg tactgcctct
    25681 tggggcactg atcgccggac ggggcatcgg tatgcgctac agggaagagt taggtgatct
    25741 gaaatcgaag atcctggaaa gtgtatacag tatccgggat atccagatct ttggtgcagg
    25801 aaaccggaaa atggaagatg taatgaagac aaacagccag gtaaataagg ctgctttagg
    25861 acttaccctt caccgccaga ccattgcctc tttccctaac tttttcatct accttgcaag
    25921 gatactgatc cttgtatgcg ccggatatct tgcatcaaaa ggcatcgata acccggtagg
    25981 aaccattgct atttcctttg ctgcaacagc ttctctatcc tcttctttca gccttacttt
    26041 tgtggtaaca agcctgttag aggcatacgg cgctgcagaa cgtattttta agatcgagga
    26101 tacattgcct gagactgagg aaccggaaca tgcggttacc tgcggcgaga tccagaccat
    26161 tgaatttaaa gatgtgactt tcgcttatcc aggtacagaa cgaaagatcc tggaacattt
    26221 taattatgtc atacacaaag gggaccagat cggtattgcc ggtgagtccg gtgccggaaa
    26281 atccacactg ctgcggcttc ttctgcggtt ctatgctcca acagagggtc agatcctgat
    26341 caacggtatt ccactggaac agatcagctt cagggaactg cataagcgga tcgcatttct
    26401 ggaacaggat acttatctgt tcgacatgac cattgcagaa aatataggga tcgcaaaacc
    26461 cggtgcttct atggaagaga tcaaagatgc tgccaaacgg gccggaattg cggaatttat
    26521 ccataccttg ccagatggat atgataccga catggggcag atgagcgcaa ggctttccgg
    26581 cggtgaacgc cagaggatcg gcattgcaag aatattgctc cgtaacccgg atatctgcct
    26641 gatggacgag ccatccagtg ctttagatgc ccttcatgaa aaggaactgc tccatacgct
    26701 gcagactgcc tatgcaggaa agacactgct gctgatctcc cacaggggaa gtacgctgac
    26761 aggatgcagc aggattttga gagctgaaga tctgaaataa agtcttcatt atcgacatgc
    26821 cccaaataca gctttccccc gccattggcg ggagattgtg catataggac attcaacaaa
    26881 aagaccagct gtaccagaca gatatgtccg tccacaactg gtctttttat aatccattat
    26941 aaacttatga aatagactct actgtataac ctgcatcttc cactgctttt ttcagcacat
    27001 ttacatcgat ctcacggtca taggaaacaa ccgctctctt gccttttaag ctgacatttg
    27061 cagatacacc gtcgatcttg tttaaagcac gcattacacg gtttacacag tgctcgcagg
    27121 tcattccgga gatctggacg atcttttcac cgatcacagg tctctctaac ttgctcggtt
    27181 tttccttgat ctcgctggct ccgccgccac cgcagcatgg gctttcgcct ttcatatgct
    27241 taatggtgcc tctgatagca aagatggcga tcaggatcaa tataaggatc acaattgcat
    27301 taaccatttc taagtccctc tatttctgta acaggtatgc cactgtttac tcttcttcgc
    27361 ggaatttctt atcttttgct tcttcccgct ttttctggat aaattttact ccctggtaga
    27421 tccccattcc cactacatag atgataatga gaagaactac catatagagc atggaaccgg
    27481 atgaatttcc cattttccgc tccttttcta acattcagtt tatgtcacat gtgctgtcct
    27541 gcctgcagac gcaggcagcg caccggatca atgtttctta gctgccttat ggctgtccat
    27601 atgatgtgca tgggcactgc cgcagctgct gcaatgtccg ctgcagccac cgcatccacc
    27661 gccgcagccg ccgtttccgc ttccacagct gctgcagctg tcttttaata cgcctgtgcg
    27721 cttccagtag atcaccagac cgcagtagcc aaatacaagg agcaatataa ttacatttgc
    27781 tatcataaaa ttcccttctt tcttaatgga aacatgaaac aatggatatc tgccatattc
    27841 attttaccac aatcatgcag ttttaaaaag gggaccggat gtttccaccc ggccccttta
    27901 ttatgccggt ttaatatacc gacgcttgtt tttcttctgc gctcattatg acgcggactg
    27961 tacagaacgc ttggaatagg tcttctgatc cttatatgga tctggacgga acagcatata
    28021 cagcatgaac agtagcacta cgaatgccac aactgtgcct gcaccgaagg aaccatagaa
    28081 taagaagctg ccgatctggt atacgcaaag agtaacagcg taagcaaata cgttctggaa
    28141 caggatcgca aaccagaacc actttctatc ctgcatctgc tgggccattg tagaaatagc
    28201 tgcaagacaa ggagagtcaa ggaggttaaa cagcaggaag ctgaatccgg ctaaagcggt
    28261 tgggaaccac attccaacac cttctgcaac agtttctgca tcttcaacgt caccagcaac
    28321 atttgcaagt acgcccatgg tagaaacgat accttccttt gcactgaaac cggagattgc
    28381 agctgcaacc ggctgccact cgccccagcc aagaggagca aagagaggcg cgatcgcacc
    28441 accaatgata gccataaggc tgtctgcgga atcttcaaca atgccaaagg aaccatcagc
    28501 aaatccaaaa gtagaaagca gccacattac tacgcaggca aggaacagga tagtaccagc
    28561 tttgatgata aagcctttca gtctttccca aacatgcatc agaacagtct tagcagatgg
    28621 gatatggtac tgtggaagct ccataacgaa tggagcaggt tttccggaga atggttttgt
    28681 tttctttaac atgatagctg ctacaagaac agctacgata cctacaaagt acatgatagg
    28741 agccatcatt ccggctacat cccagccagt tacatagcca gccataacgc cgcccattaa
    28801 agcgattaca ggaagcttgg caccacatgg gataaaggtt gctgtcatga ttgtaagtct
    28861 tctgtcgtta tcctgctcga tggtcttgga agccatgata cctgggatac cacaaccgga
    28921 ggagatcagt aacgggatga agctcttacc ggacagaccg aagtgacgga atacacggtc
    28981 cataacgaat gcgatacgta ccatgtaacc gcagtcttcc aggatggaca ggaacaggaa
    29041 caggatcgcc atctgaggaa cgaaaccaag tactgctccc agaccgccga tgataccatc
    29101 aacaaccagg cttaagatca gatcgcttgc gccggcattc tcaaggaaac cgccaacaac
    29161 ttcctgaata gcacctacaa atgtatcgtt ggtccagtca gttacgaatg taccgatggt
    29221 agttacggag atgtagtata cgatccacat taccagcatg aagatcggga tacctaagaa
    29281 acggttggta acgatctggt cgatcttatc agatacggtc agcttctcgc cgctcttctt
    29341 aacagcagtc ccaactacct tctggatgaa tttgtaacgc tcatcagtta caatactttc
    29401 catatcgtca tcatgagctt cttcgatggc ttttcttgcg ttctcaacaa tagacttgcc
    29461 ggaagcagaa agaggggtgc tctcaactac cttgctgtca ttttcaagaa acttaacagc
    29521 ataccatctc ttcttgttct cagtgatgtc tgccggcagt acgccttcaa cctcagctac
    29581 tgctgcttct aattccttag aaaagatctc cttaggaaga tcagcttctt tcttcttagc
    29641 aaccttaact gcttcgtcga tcagcttatc aagaccggtc tgcttcaatg cagatgtttc
    29701 aacgatcgga cagtcaagaa gcatggaaag acgctcaatg tcgatcttaa tacctctctt
    29761 ggcaagcaga tctgccatat ttaatgcgat cactaccgga ataccggttt cgatcatctg
    29821 ggtggtcaga tataagttac gctcaatgtt tgttgcgtca accaggttta caataacgtc
    29881 cggatcatcc tttaacagat aatcacggct gacaacttcc tccaaagtat aaggagacag
    29941 tgaataaata cccggaaggt cagtaacgat cacttcttcg ctctggctct tgctttttaa
    30001 ttttccctct tttttctcta ccgtaacacc aggccagtta ccaacatact gattggcacc
    30061 tgttaatgca ttaaacatag ttgtttttcc gcagtttggg tttccggcaa gcgcgatctt
    30121 aattgccatt tttcatttac ctccatgagt ccattgcaaa ggactctttt taattctcac
    30181 ggtgtgttgt gtccgtttat tttacttcta cacactgagc atcatttttt cttacagaaa
    30241 gctcataacc tcttactgta atttcaacag gatctcctaa aggagcaact tttctaatat
    30301 aaagctcact tccttttgtg atccccatgt ccataatacg acgtttgtaa gcgccatcac
    30361 ctgtgatctt agtcactact acagtgcttc caactgctgc atcacctaaa gtcattgcta
    30421 tgtctccttt cttctctttg tcatgttcat ttatggctat atcaccctta ggcaacatgt
    30481 ccatttaaac catgatatgt cttgccatat cctgatttat tgcaactctt gaatccttaa
    30541 tattgacaat aacatttcca ccgatggcag atacaactgt gatctctgct ccggcaacaa
    30601 atcccaggtt ttcaagaaaa cgctttgttt cttcatttcc gcctactttg cggatggtat
    30661 ttgcttctcc gatcgaagcc attgttaaag gcatcttctt cactcctttt ttcaagttag
    30721 tttttcctat tcaaggttag tcattgctac cccgatcatc ttagttagaa tacgctaatt
    30781 taagttagtt gtcaataacg cagactattt atatgctgat aatatttctc aataaggtgc
    30841 atttttttta tttttgctgt ataattcata aagatgttgg gggcaaaagg tattttgacc
    30901 cctctgatac cgaattgcta cgtgctttgc actcccgcaa ttcttcataa ataaactgcc
    30961 gtcagccaca aaggagagct tctatgacat tacagcaaat gcgatatttt attggtgctg
    31021 ctgaatccgg ttcgatcagc gaagccgcta aacgatcttt tactgcccag tccagcattt
    31081 ccggtgctat taaagaagtg gaaaatactt atgggatcac tgcttttgta agggacagca
    31141 aaggggtccg ccttaccaga agcggagaag agcttctgat cgaatttaaa tggatattaa
    31201 accgactgga ttatctggat gaaaaattca atggttccaa aaatcgtcct ttaggtttat
    31261 ctgtttcctc ccagcatcat atctgcggta tgaattcttt tgtatcgatc atcaacagcc
    31321 tgaatacagg tgccggacct tatcactgcg gttttcacga atgccgcaca tctgaagtta
    31381 tggaccgggt agaacgcggt ctggatgatc ttggcatcgt ttttgttaca gaatacagca
    31441 agggccagat gatgcaggag ttaaggaaca gaggcatcat tttcaatcat attacctatc
    31501 agacttccca tgtatacata tgccaaaacc accctcttgc tggcagccgt gaaatcgaca
    31561 tagaagatct gatccagtat ccttttatca cctatgaccg gtcttcagat acgaattctg
    31621 cttatactga cctgatcgtc ccttatcatc agttaaacag ggtgatccag gtttctgacc
    31681 gggctgcttc ttattctgtt atgcgttact gtcagggatt tgctgtcgga agcggctatc
    31741 ggccgtttga tgataactac agcgatatcg tttctattcc tattaaaaac gggcggcggc
    31801 ttgaaatagg atggatcgta cgccaaaaat atactctttc tgatacagcc agccgttttg
    31861 tggatctttt aatgcagatt gaatcagttg tcctgtgacc ttaaagccgg ttttcgtaaa
    31921 aagcgatgga aatccatcgc tttttgacgt attcgcagaa aacgcttcct gtgttatgtt
    31981 taaatcacaa aaataattcc tgcacagaca gaagaaaggg gtatcttgta tggaaaagct
    32041 gaaaactgtt attaacaagt actggaaggt ttatgttgta gccttgatcc ttgctacggt
    32101 atgcgacctg atcggtacac ttaagttcaa tattggaatt ggtacactta ccttatttcc
    32161 aatggtattt gccacgatct ttggaggcct tttaggaccg gacgccttta agcttttcaa
    32221 aacagaagaa tcaaagcttg gaggaagcct tgttttagta gcccttgcac cttttatggc
    32281 aaaaatggga atctcagcag gtggaaatct tgcaaaactg gttgctgtag gacctgcgct
    32341 tctgctgcaa gaatttggta atctgggaac catccttatt tcacttcctc ttgcactgct
    32401 gcttggtctg aaaaaagaag ctatcggtgc ctgctattcc atcaaccgag actccaacct
    32461 tggattaacc actgatatct ttggaccgga tgccccggaa acccgcggaa cctttgctgt
    32521 atatattgtt ggttctgtta ttggtaccgt ttttatcagt atcctggcca gcatcgtagc
    32581 ttcctggcat atttttgatc ctattgccct tggcatggct tgtggtgtag gaagcggatc
    32641 aatgatgact gccgctgccg gaacattagg tgagatctac ccgcagcagg ctgaaagtat
    32701 tatgatcatg ggcggcgcca gtgatatgct tactggtatc accggaattt atatgggaac
    32761 attcgtagct cttcctctta ctaaaaaact gtatgcaata ctggaaccta aaatcggtca
    32821 caaaaacaat aaggaggcta aataatggtc agaaaatact ttttacagca gcttggactg
    32881 cttatcatgg tttctgttat catggtactc acaaatatac tgggctacaa aatgccggta
    32941 ggtgattcta ttgccgggat cctggtatta tgcgctattg ctctcttcgg acttacagta
    33001 agtaaattta tgagccagtt tataaagctt ccatctatga tgtatgtatc tcttaccgga
    33061 ctgcttcttg catgccctat ctcaccggta aaagactttg tgatcggtgt tacatcccag
    33121 gttgcattta tggctcctac cactgcatta ggcgcatttg ccgggatctc acttggaaaa
    33181 gacctgaagg attttgctaa aatgggatgg aaactggtgc tcatcacctt atttgtaatc
    33241 accggaacat ttattttctc tgccttagta gcagatgtta tcttaaaact tacaggtgcc
    33301 atttaacatg agcgaggtaa atttatatgg aaaaatatga tattttaatt aagaactgtc
    33361 aggttttaaa ccctgatatg acggtttcat ctacctgttc tgtaggaatt aaagacagct
    33421 ggatcaaaaa gatcggtcct acagatgaat tgatggctga cagctctgcc agtgaaataa
    33481 tcgatggcaa aggaaagctt gtcatgcccg gcatggtaga tggccataca catacctgcc
    33541 agcagctttt aagagggcgt gttgctgatg aataccctat ggtatggacc cgttttctgg
    33601 ttccttttga aagcaatctc cagccagaag attcttatat cagcggcctt ctggcatgtc
    33661 tggaaatgat caaaagcgga acaactgctt ttgctgattc cggcggtgta catatggacc
    33721 gggttgcaga tgcagtcatt gaatctggca tgagagctgc cattgcaaaa tcaaccatgg
    33781 atatgggaaa tgccattact ggtgcaatga aagagacagc taatgaggct atcgaaaaca
    33841 caaagaaact gtatcagaaa tatcaggggg ctggcgatgg ccgtgttgat atctggtttg
    33901 ctattcgtca ggtaatgacc tgttccaggg aactgatcgc catggtaggt gaatctgcta
    33961 aagaattcca taccggcatt catgcccatc tgtgtgaaca taaagacgag gtcagcttct
    34021 gcctgcagaa ttaccaaaaa cgccctgcag aatttctgga agaaatgggc attcttggtc
    34081 ctaatcttct gactgcccac aatgtaatgc tgtcagatca cgatattgcg ctgatggcag
    34141 aacgtggtgt gaaaatgatc cattgcccaa gagccaacct ggcaaaccac ggttttccaa
    34201 aagctcccca gattctggaa gccggtgcaa gccttggctt aggctgcgat ggtgctgcac
    34261 cttccaatct ggatattttt gacgaaatga aggttcttcg ctatgctatg atggcttact
    34321 ggggaattcc atcttttaat cctgtagtca tgacctgtcc tacactttta aagatggcct
    34381 cccagggcgg tgcaaatgct cttggacacg gtgatatcct tggatccgtt gaagaaggca
    34441 agaaagctga cctgatcctg ttaaatattg accagcctca tatcactcca agccagaatc
    34501 tggtaaatac cattgtagat gctgcaaacg gacatgatgt aaccgattct atcatcaacg
    34561 gcaagatcgt aatgaaaaac cgtgaggtcc ttactctgga tgaagagcgt atccgctttg
    34621 aagctgaaaa gcatatgaat gatattatta aacgggcata ctaagatata aaaaatgacg
    34681 gcactgtttc cagtttggga agcggtccgt catttttaca gcattttttg atcatatata
    34741 aggctacttt acattttttc tttttttatt accagtttac cacattcacc ggatctccag
    34801 ctaaaaactg tttaagattt tccacagaca tatccatgat cctctgcctt gcttcctttg
    34861 gcgcccagga aatatgaggg gtgatgatac agtttttagc tttcagaaga ggattgtcac
    34921 tcttaatagg ctctgtactt accacatcca gtcctgctgc atatactttt ccactgttta
    34981 atgcatctgc cagatctttt tccacaatca gctgtccacg ggagttatta atgatgataa
    35041 ctccatcttt catcctggca atattatctt tattgataat gccttctgta gacgggaaca
    35101 atgggcaatg aagcgagatc acatctgatt ttgtaaatat ctcatccagt tccacatact
    35161 cagcaatagc acgtccttta tcatttggat gggaatcata agccagtaca ttcataccca
    35221 ttgcctttgc tatggttcct gttttctgtc caatacggcc aaaaccaata atccccagtg
    35281 tttttcctgc cagttcgatc agcggatagt cccagaagca ccagtctgga gcattttccc
    35341 atctgccctc ttttacagcc ttatcatgat gtccgatgcg atggcagatc tctaaaagaa
    35401 gagcaatggc aaactgtcct acacagtccg ttccataagc aggaatattg ctgacaacga
    35461 ttcctttttc ttttgcataa ttaacatcga tcacattgta gcctgttgca aggacactga
    35521 taaacttaag atccgggcat gcatccatgg tttcttttgt gatcggagtc ttatttgtaa
    35581 acacaatttc tgcattgcca atacgcttca ctgcatcctc tgccaatgtt ctgtcataaa
    35641 ccgtcaattc tccgtatttt ttcatctcat cccaggaaag atctcccgga ttttctgtat
    35701 atccatcaag tacaactatt ttcatctgca tttctccttt gtatgtaatg tacttacaaa
    35761 aactttttat actatagtct tattgtttct ttatcccatc ccgatacatc gacggtgaaa
    35821 ccccggtgac attcttaaat gcccggttaa agtgggcggc atcttcgtag cctactgcca
    35881 gggcaatgtc taaaagcttc agggacgagt ctttcatcag ttccttggcc tttgtcatgc
    35941 gaagctgaag tatgtaatct ctcactgtca tatccagctg ctttttaaac aaactgctga
    36001 agtaattggg gtggagatgg aactgggcag caatgtctga aaggagaagc tgttcagaac
    36061 agtgcttttt catgtattcc tgtgcctttt ttaccacata ttcttctttc ttttcctgct
    36121 gttttttcag ctgttccata taaagcagca ggatctgttc cattttctgt cttaactgac
    36181 tgatgtgggg ctggttttta atctgggcag ccaggtcatt atctgacagc ttgtaacctg
    36241 ctataatagg gatcagggct gtatttttct gcattaatgt gtagagaaat gcggaaaata
    36301 cctgcttaaa ctgttcttta gtcacagctt cctgggataa atgggaaaag atttcctgga
    36361 tcttatcttt tgtgcccttt tcgtccatgg acaaaagccc ggctgaaaga tgttccattt
    36421 ctttttccag atcttctttt gtataataag gttttttggc cggaatatca aaataatccc
    36481 cctgtgtttc cggacgcaca cagcttatct cttctgcctg cagctttacg gaactgtctt
    36541 ttaaccgcgg gatcagcaat ctgagcatgg acacaaaatt ctgtatattc acaggcttta
    36601 acagataatc ataagccccc agcctcaggg cattgcggat ataggtataa tcatcatagc
    36661 cactgagcat gatcaccaca ctgggaatct ggtttttgct gatcagctcc agaagactga
    36721 tgccatccag ttttggcata cggatatccg aaatgatcac atgatagtaa tcttctgtaa
    36781 gacgttttaa agcatcactt ccatcgcagg cactggatat aacaaagata ttttcaaaat
    36841 tttgctttat aatactccca agaccatcta caatgttctg gtcatcatct actaatagga
    36901 tccggtacat cctgttcctc cttcttcgtc cgctcttctt ccatatcatc actcatgtta
    36961 tctttccatg gaaaaccaag catgatcata gttcccattc cttcagcact ttcaatggat
    37021 accttatact ccggaccata gtttaagctg atccgcctgc atacattacg gagtccgatg
    37081 ctgttgctgg aaggactgtc tatatcatgg cgcagcatct ctaactgttc ttcactcata
    37141 ccacatccat catcggaaac ctggatatat aggcggtcct cgtctctata tcccaaaata
    37201 tcaatatgca ggatctgatg actgtctgta ataccatgga tcactgcatt ttccacaata
    37261 ggctggaata ccagctttaa aacatggcag gaatacagct ccctgtcaat gcagatgtta
    37321 aactggatcc gttcattgta ctggtatgca agaatagccc ggtagatatt taactgatgg
    37381 atctcttctt ccaaaggcac ttcttttgtg gtattccgaa ggccatactg aagattctgc
    37441 cctaacagtt ctgccatggt agctgtttca taatcctggc acatataagc cttcatgtgc
    37501 atgatctcca aagtattata cagataatgg ggattgatct gggtccttaa aagctgcagt
    37561 tctgtttcct gtttttcctg ttgctttaca aatacctcat ggatcaacag atcaatacgt
    37621 tttgacatta tattaaaaga ggataccaga gtctgaaatt cctctggaag tccttctttt
    37681 gaaactttat ttatgtgacc cagttcaaaa tgattgcagg cttcgatcag acggtttaat
    37741 gcctttaaga tccggcctac catctgaaag atcataaata tgattccacc taagatcata
    37801 cctatgggaa tgatatttag catctggatc cttcccatat ttccaaaaat gtcagacaga
    37861 ggagtctgta ataccagagt gtagccatta gcgtggccga tcacattgta tacacaggag
    37921 tttttatcac tgtgctttaa ttttctgctc tggtaatttt cttttcgttc tgtttttttc
    37981 agattttctt ttacaaatcc ctgatagctt tcttcctgtt ctatatttcc gtacaaaagc
    38041 ttcccgtctt tatataccgc aaatctctga ttcggtgtct gacggttctg ctcaaaataa
    38101 ttctccagat aagaagtatc tattcctgcc acgcaatagc ctacgatctt gatcgtattc
    38161 aggtccagaa tgcctctgcc aatacacaat gaac
//
LOCUS       DBMDUA010000007        33893 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1027_length_33893_cov_3.413056, whole genome shotgun sequence.
ACCESSION   DBMDUA010000007 DBMDUA010000000
VERSION     DBMDUA010000007.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 33893)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 33893)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33893
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1027_length_33893_cov_3.413056"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(492..1862)
                     /locus_tag="ACZPCL_01225"
     CDS             complement(492..1862)
                     /locus_tag="ACZPCL_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HIR1161145.1"
                     /translation="MRKKMKAAWFLAALLSAGLAAFPAMAAQGWAQENSSWVYYDSNG
                     NKVYNEWKKGADGQWRYLNGEGYMAVNAWADSDYYVDSNGIMVTDKWLKTTSDGGEEE
                     WYYFGSSGKTIDDTWKKINDKWYHFDGNGRMETGWILDDMYYTGDDGVMRTGWQKLYP
                     PDNYEEDSNKVIPGAEGGTLDYGEDGQYWYYFGTNGKKYVPSDGNDNGDYGTRKIDGI
                     YYCFDDDGILQMGWKNVRGSSDSIKDYMYFGSDGKAKIGWYSIEPPEDLEGYDGDVEW
                     FYFANNGKPKAADSDHLTTQDLTKISGKTYLFNKRGNPVYGLKKVYIGSGDSNWTAFY
                     FGTKTQSCVQRGKMKVEEDDGNKTDFYFSENGRGYTGVKDNYLYYKGRLQKATDGQKY
                     VCYKIDSSNYVVNSSGKVMKNSTVKTSDGVKFKTSSSGSLSTADDDNDVSSYFTEPEE
                     PICTED"
     gene            2150..3010
                     /locus_tag="ACZPCL_01230"
     CDS             2150..3010
                     /locus_tag="ACZPCL_01230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1161146.1"
                     /translation="MWLADGWKDYEVIDCSKGEKLERWGDYLLVRPDPQVIWDTPKKH
                     KGWKKMNGHYHRSSKGGGEWEFFDLPEQWTINYKELTFNLKPFSFKHTGLFPEQAANW
                     DWFGDKIRKAGRPVKVLNLFAYTGGATLAAAEAGASVTHVDASKGMVTWAKENAHSSG
                     LSDAPIRWIVDDCVKFVEREIRRGNHYDAIIMDPPSYGRGPKGEIWKIEDAIHPLVKL
                     CVQLLSDDPLFFLINSYTTGLAPSVLSYMMGVEIVPKFGGTVKAEEVGLPVSQNGLVL
                     PCGASGRWEK"
     gene            complement(3050..3949)
                     /locus_tag="ACZPCL_01235"
     CDS             complement(3050..3949)
                     /locus_tag="ACZPCL_01235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR1161147.1"
                     /translation="MNRARMEIHCDEAALKGITGKGVGVAVLDTGIYLHEDLKGKIQG
                     FKDFVHGKKLPYDDNGHGTHIAAMIAGSGLASGGLLKGIAPGSSITAIKVLDHRGNGY
                     AADVLMGLRWIREHRKEYGIRIINISVGSLTRREMTENSALVKGVNAAWDDGLVVVVA
                     AGNHGPAPGTVTTPGISRKVITVGCSDDDREVEVMGSRMVDYSGRGPTNACVCKPDLV
                     APGCGIISCCNEPGRYFIKSGTSMSTPLVSGAIALLLEKYPSMSNKDVKLRIRERAVD
                     LGLPHNQQGWGKLDVKRLLADEI"
     gene            complement(4282..4899)
                     /locus_tag="ACZPCL_01240"
     CDS             complement(4282..4899)
                     /locus_tag="ACZPCL_01240"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1161148.1"
                     /translation="MIRNIVFDMGKVLVDYSGMPVCRHFTDNEALAKRVCTAVFDSPE
                     WVLLDMGVITEEDGIKKMISRLDTEEEKELAVKSFDHWHEYNLVQKPEMGDLVRDLKA
                     KGYGIYLCSNASVRMLTCYKEVLPAVECFDGILFSAEVLCMKPQKEMYGHFFERFGLK
                     PEECYFIDDLPNNIAGAKACGMDGYCFADGDVEKLKRTLYESVLS"
     gene            complement(5018..5296)
                     /gene="yajC"
                     /locus_tag="ACZPCL_01245"
     CDS             complement(5018..5296)
                     /gene="yajC"
                     /locus_tag="ACZPCL_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR1161149.1"
                     /translation="MQGMGFIVIYIVFFAALIYFTSIRPQKKERQKQQEMLSSVAVGD
                     TILTTSGFYGVIIDMTDDTVIVEFGSNKNCRIPMQKAAIVQVEKPETK"
     gene            complement(5370..6500)
                     /gene="tgt"
                     /locus_tag="ACZPCL_01250"
     CDS             complement(5370..6500)
                     /gene="tgt"
                     /locus_tag="ACZPCL_01250"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR1161150.1"
                     /translation="MKYQVITKDGRAKRAQMETVHGTIQTPVFMNVGTVGAIKGAVST
                     DDLRQIGTQVELSNTYHLHVRTGDKLIKEYGGLHQFMNWDKPILTDSGGFQVFSLTDL
                     RKIKEEGVYFRSHIDGHKIFMGPEESMQIQSNLGSTIAMAFDECPPSHADYDYIRNSV
                     DRTTRWLKRCKTEMDRLNSLPDTINKEQLLFGINQGGVVDEIRIQHAETIKELDLDGY
                     AIGGLAVGETHEEMYHILDVTVPHLPEDKPVYLMGVGTPANILEAVERGVDFFDCVYP
                     SRNGRHGHVYTNHGKLNLFNQKYELDHSPIEEGCQCPACRSYSRAYIRHLLKAKEMLG
                     MRLCTLHNLYFYNKMMEEIRDAIEEHRFAEYKAAKLAGMEGK"
     gene            complement(6504..7904)
                     /gene="scfB"
                     /locus_tag="ACZPCL_01255"
     CDS             complement(6504..7904)
                     /gene="scfB"
                     /locus_tag="ACZPCL_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HIR1161151.1"
                     /translation="MIHQYINNGYHIVLDVNSGSVHVVDPVVYDAIAAVSEQVPEMDK
                     PEKLSEEVTANVHKKLDETYGSEMVCEALEDIQELIDTEQLLTRDIYRDFVLDFKKRK
                     TVVKALCLHIAHDCNLACRYCFAEEGEYHGRRALMSYEVGKKALDFLVANSGNREHLE
                     VDFFGGEPLLNWKVVKQLVEYGRSLEEANHKKFRFTLTTNGVLLNDEIMEFCNREMSN
                     VVLSLDGRREINDKMRPFRNGSGSYDMIVPKFQKFAESRGQKNYYVRGTFTRNNLDFA
                     DDVIHYADLGFQQMSMEPVVADPAEDYAIREEDIPQILKEYDKLALEYIKRKKEGRGF
                     NFFHFMLDLTAGPCVAKRLAGCGSGTEYLAVTPWGDLYPCHQFVGNEKFLLGNVDTGI
                     VNTETRDEFKLCNVYAKETCKDCFARFYCSGGCSANAYNFSGSINGAYEIGCEMQKKR
                     IECAIMIKAALADEEA"
     gene            complement(8029..8175)
                     /gene="scfA"
                     /locus_tag="ACZPCL_01260"
     CDS             complement(8029..8175)
                     /gene="scfA"
                     /locus_tag="ACZPCL_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HIR1161152.1"
                     /translation="MKHVKTLNANTLKDTMKKGGCGECQTSCQSACKTSCTVGNQSCE
                     NSAR"
     gene            8307..8669
                     /locus_tag="ACZPCL_01265"
     CDS             8307..8669
                     /locus_tag="ACZPCL_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HIR1161153.1"
                     /translation="METKVIKPLLRSLFISYALSALFLGALAFALYKLRLGESLVNLL
                     IFAVYFAACFAGGFFTGRKVRNRRFFWGLLSGMFYFLILFAASWAMDPRASIQAARSL
                     MVLGVCAFGGTLGGMLSF"
     gene            complement(8659..9129)
                     /locus_tag="ACZPCL_01270"
     CDS             complement(8659..9129)
                     /locus_tag="ACZPCL_01270"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR1161154.1"
                     /translation="MILPWLTGGIAALDLFIKDEIEREGEEEFPRDLPGTKGLIRLHK
                     SYNSGFPFGFLKERPELVKGIPLAVTSAAAGALGAFMTKKGCLAQKIGLSMVLGGAIS
                     NLYDRLVKGYVVDYFSIEFKRLKKVIFNLGDIFVFLGSLVFLIGELLEDIDLKN"
     gene            9281..11068
                     /locus_tag="ACZPCL_01275"
     CDS             9281..11068
                     /locus_tag="ACZPCL_01275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR1161155.1"
                     /translation="MAECILNPASQDVADFIYRFGSLQNNLRDPEQFPCVDYVSNDYA
                     VFYTELENALPLSLERQPYYSIPSLFTLLDDSAMEAAGIVSALQTPALSNMGSGVIIG
                     IVDTGIDYTSPVFRKSDGTTRILGLWDQTLPEDPSVLPPGVPEYYPMGGASYGTEFTH
                     EEINEALTLEDPFSLVPSKDTDGHGTFLAGLAAGTAFPLQNFTGAAPMADLAIVKLKP
                     AKKYLRDYYLISEDTPAFQENDIMMGIKYLRVIADRFRRPLVTLLGLGTNYGSHTGTS
                     PLSQVTQNYGGFFGIATVIAAGNETGLAHHYAGRFSADTSFEDVELRVGEEEGKRGFI
                     LELWSSAADLYTVGFVSPGGERISRIPILSNNETRIPFLLESTVITVSYQLIEAGSGS
                     QLVSMRFERPSPGIWTIRVYNTQFLTGEYHMWLPVQGFISDETVFLKPDPSNTITVPG
                     NSRLPITTGAYNHRNNSIYIHSSRGYTSRDYVKPDLAAPGVEILGPSVRISAAFRPGL
                     SSSVAAGTAPAFSRRTGTSAAAAITAGAVANLLSWGIVMGNYPTMSEASIKSYLIRGA
                     ARNPSLTYPNKEFGYGTLDLLQTFLRLRE"
     gene            complement(11092..12471)
                     /gene="clpX"
                     /locus_tag="ACZPCL_01280"
     CDS             complement(11092..12471)
                     /gene="clpX"
                     /locus_tag="ACZPCL_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR1161156.1"
                     /translation="MENGMTNGSQDDNKNNDNYEKICYMCRRPESKAGPMISMPGGMC
                     LCHDCMQKAFDTVTKGGMDFSKMPNMPYMNMNFSDLSQMPPVNEIPQSQKLKKKKEPE
                     KQLTMKDIPAPHVIKGKLDEYVIGQDKAKKVMAVAVYNHYKRAFLGGSDPDGVVIEKS
                     NILMIGPTGSGKTYLVKTLAKLLDVPLAIADATSLTEAGYIGDDIESVVSKLLAAAGN
                     DVKRAEKGIIFIDEIDKIAKKKQTNTRDVSGESVQQELLKLLEGSTVEVPVGSNQKNA
                     MTPMATVNTDNILFICGGAFPDLENIIKERLTKKSSMGFGAALKDSYDNDPDLLSHVT
                     NEDLRAFGMIPEFLGRLPVNVTLKGLDKDMMIRILKEPKNAILRQYEKLLALDEVKLV
                     FEEDALSWIAEEALKRGTGARALRAILEEFMLDIMYEIPKDPNIGSVVVTRPYLEKKG
                     GPLIQVREG"
     gene            complement(12499..13884)
                     /locus_tag="ACZPCL_01285"
     CDS             complement(12499..13884)
                     /locus_tag="ACZPCL_01285"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR1161157.1"
                     /translation="MRKNNLLKKTAWIVLSAVLFGTVAGTVMTGIQIASSGMVSQFFA
                     IVASDEDTVKAEGEALPPPEPFPGEKIPETFPQAADIPAPSTDVSEIVEEAMPTVVAV
                     ASTAVYQTPDYGYSWFFGGGSQSYEVPSSGSGIIIGKNDTELLIVTNNHVVKDTVSLK
                     ITFVDDTAVDAAVKGTDADTDLAVISIPIDQIPQETQDKIAVARLGDSDSLKVGQGVI
                     AIGNALGYGQSVTVGYVSALNREVQTSDGNTRVLLQTDAAINPGNSGGALLNMKGEVI
                     GINAAKYSSTEVEGIGYAIPVSGVQEILDELMNRKTRSEVEESRRGYLGIQGTTVDEE
                     TAATFDMPKGVYVYKILEDGAAAGSELREKDIITKLDGMTVKSMQELQKFLKGYEMGE
                     TIELLVQRQEEGRYKEQQIQITLAGLPEGEGQQEKQDNREQPGETAPSRREDSNPWSF
                     PGNGFPWGRMW"
     gene            complement(13988..15613)
                     /locus_tag="ACZPCL_01290"
                     /pseudo
     CDS             complement(13988..15613)
                     /locus_tag="ACZPCL_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(15763..17181)
                     /locus_tag="ACZPCL_01295"
     CDS             complement(15763..17181)
                     /locus_tag="ACZPCL_01295"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HIR1161158.1"
                     /translation="MIKDNQKSLNHVQVLLDAVVTAVAYILAWFIIVSGKVVPLRGAT
                     LEPGPYFLALIFIVPVYLILNGCFHLYVPKRIQGRRVEFANICKANVIGLMLFTLILF
                     GGRSFIVHLGYFSTRMLLVFFVLNILLLAGERLAIRLFLRSLRTNGYNQKHVLLIGYS
                     RAAEGFIDRVTLNPEWGYHIQGILDDHKKAGYVYKKVQVLGPISHLETFLAANTLDEI
                     VITLSIGEYANLEQIVAACEKSGVHTKFIPDYNNIIPTIPYMEDLQGLPVIHIRHVPL
                     TSVFNATIKRMVDICGALFGIILFSPLMLLTAALIKLTSPGPIIYSQERIGLHNRPFK
                     MFKFRSMEVQDPNKEKNQWTTPHDPRVTPVGRFIRKTSIDEMPQFFNILIGDMSLVGP
                     RPERPLFVEKFKEEIPRYMIKHQVRPGLTGWAQVNGYRGDTSITKRIEHDLYYIENWS
                     LGFDFKIMFLTIFKGFINKNAY"
     gene            complement(17310..18296)
                     /locus_tag="ACZPCL_01300"
     CDS             complement(17310..18296)
                     /locus_tag="ACZPCL_01300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1161159.1"
                     /translation="MSNKAKVTVVIPNYNGLKFMDPCFKALDLQLCRDFDILVVDNGS
                     TDGSVEWLKEHEIPSIFLEKNTGFSGAVNVGIKASKTPYVILLNNDTEPDCHYIGEMI
                     KAIERSPEIFSVSSKMIQLYDHSKMDDAGDMYTLLGWAFQRGVGRPATGYNRSCPVFS
                     ACAGAAIYRREVFETIGYFDEMHFAYLEDIDVGYRAKIYGYKNVYCPSAMVYHVGSGT
                     SGSRYNSFKVRLAARNNIYLNYKNMPWPQLLLNALPLGLGILVKYGFFKKIGFGKDYL
                     DGLKEGVKTAKLCKKVKYNRDHLKHYAAIEWELCTGTLLYIREFLARQAAKR"
     gene            complement(18289..20514)
                     /locus_tag="ACZPCL_01305"
     CDS             complement(18289..20514)
                     /locus_tag="ACZPCL_01305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1161160.1"
                     /translation="MKYKVDVVRIRENSITLNGWALGKTPESKVTFRVEDEHHQPVKC
                     KMVSTRRDDVSQIYFKKVIDKEFGFDIQFPYERGKSYWLLIRCDGRQAKIKYNEELIT
                     KRASVAHKRMEKIKDLMNMETVHVALDFWKENGLRALIKKSCHKIQGLDNDYDYGEWY
                     ELTKPTEEELKAQRETHFEKEPLFSVVIPVYKTPERYLKEMLDSILDQTYGNWEVCIA
                     DGSPRGQDVEKVLKKYAQKDSRIRYEILGGNRGISGNTNAALAMAKGEYVILADHDDT
                     IPPQAFYEVAKAINKHPDCDVLYSDEDKLDMDGKALFDPHFKPDFNPDLLTSVNYICH
                     LFVVKKELLDQVGGFRQEFDGAQDYDFIFRCTEQAKEIVHIPQVLYHWRCHQDSTASN
                     PESKMYAFEAGSRAIMAHYERMGIPAERVEKGVDYGIYHTTFKIKGEPLISIIIPNKD
                     HSKDLDVCVRSILEKSTYRNLEFIIVENNSTEPETFAYYKKMEEKHPSFHVVTWKEGF
                     NYSAINNFGASFAKGEYLLLLNNDTELLEPDSIREMLGFCQREEVGIAGARLLYADDT
                     IQHAGVVIGFGGIAGHTFIGLHKAENSYFHRAMCAQDYSAVTAACLMTKKSVFEAVGG
                     LSTELAVAFNDIDYCMKVRALGKLVVYAPYATLYHYESKSRGLEDTPEKVARFNREVA
                     IFIKKWPDIIKNGDPYYNPNLTLRKSNFALRDLLKEKIGEPYDLSVYDKFAPEEKKSD
                     E"
     gene            complement(20504..21481)
                     /locus_tag="ACZPCL_01310"
     CDS             complement(20504..21481)
                     /locus_tag="ACZPCL_01310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1161161.1"
                     /translation="MKKISVLIPCYNEVENAGPISKAVTDILEKELPQYDYELVFIDN
                     DSTDGTRDVIRGLCKENPRIKAIFNARNFGQFNSPYYGMLQVTGDCVIEMVADFQDPV
                     EMIPKYVHEWEKGYKIVIGIKTSSKENKFMYWLRTCYYKTIKKMSDVEQIEHFTGSGL
                     YDREFIEILRKLDDPTPFLRGIVAELGYKRKEIPYEQPKRRAGKTHNNFYRLYDAAML
                     SVTSYTKEGLRLATIFGGVCAAGSMIIALIYLVMKLIWWDRFPAGMAPMLIGMLFLGS
                     VQLFFIGFLGEYIMSINQRVMKRPLVIEEERLNFDQKDENVKDGKANEV"
     gene            complement(21478..22434)
                     /locus_tag="ACZPCL_01315"
     CDS             complement(21478..22434)
                     /locus_tag="ACZPCL_01315"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR1161162.1"
                     /translation="MRIVITGATGFIGSNLAKLFLKKGAEVFVLVRPESTHLDVLPKD
                     EKLHVVSCGLSNVMDCVSYIKEADAFFHLAWGGVNRQEIDSPEVQAKNVAGSLDCIRA
                     AKALNCRMFMDAGSRVEYGLTDGFMQEDIECHPVNQYGKAKWEFYQKAVPLCEELGLH
                     YVHLRFFSVYGTGDHPWSIISTLVRELPAGNKVSLSACRHQWNFMYIDDAVEAVYELY
                     EQLPQSADAGVKKNGLVSTIVNIASKDTRVLKDFVEEIHSIAGGAGELEYGTFVQAKE
                     GALSIKPDISRLTLLTSGWQERFTFRQGIEIMMKKEKENKNL"
     gene            complement(22446..23579)
                     /locus_tag="ACZPCL_01320"
     CDS             complement(22446..23579)
                     /locus_tag="ACZPCL_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1161163.1"
                     /translation="MKRCIACGAPLWETPLLTLDNMPASAQHMPDAEHVGADKGLTLD
                     LCQCSGCGLVQFDCEPVDYYRDVIRAGGYSTTMAELRRKQYKHLIETYHLEGKRFIEV
                     GCGQGEFLQVLKEFPVEVHGIEHDPALVELAKSKGIDVVQGFTETEDTVFPGGLYDVF
                     LSFNFLEHQPDPVTMLRAIWNNLEDDAMGLITVPSFEYIMEHSSYYELIRDHIAYYTF
                     DTLTSLMERSGFDVVEASMINRDTLSLIVRKRPQVETENLLECYVNLREEMNTYMKYL
                     RAWHKTVCIWGASHQGFTLAATTCLGENAEYIIDSAPFKHGKYAPASHLPIVPPEYFE
                     AHPVDAIVITAPGYTDEIAGIIRKRFGDQVEIRAMRSNHLEMV"
     gene            complement(23581..24552)
                     /locus_tag="ACZPCL_01325"
     CDS             complement(23581..24552)
                     /locus_tag="ACZPCL_01325"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR1161164.1"
                     /translation="MTVIVTGATSFIGRAVVKALLEEGCRVVAVSRPASAGAGKLQEL
                     FEDLKKQAGAEKKVFGSLELCFCELGEIEKLEKIYKKEELQAKAWLHLGWDGAGSDKR
                     KDVKLQEKNIGYALQALHTAAALGCKRFLFSGSQAEYGICNEWMKEEQECHPVSEYGK
                     DKVLVYQQATKAAKELGVDYIHTRIFSVYGPGDHPWSLIETCIRTFLANGHMEMGPCT
                     QQWNFLYVQEAAQILVKLLLGKVPAGVYNVAGEDTRPLKSYIEEVYELCGRKGSYEFG
                     YRPPNAEGCVSLMPDLTKLKKAIDFHQQVSFKEGILETIKEAKKENI"
     gene            complement(24606..26612)
                     /locus_tag="ACZPCL_01330"
     CDS             complement(24606..26612)
                     /locus_tag="ACZPCL_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR1161165.1"
                     /translation="MRMKVSNYISEMLVKAGIHQAFMVTGGGAMHLDDALGHQEGLHC
                     IYDHHEQACAIAAEAYARIHNEMAALCVTTGPGGTNAITGVLGAWLDSIPMLVLSGQV
                     RYDTTARWSGTGIRAMGDQEFDICKAIDCMTKYSEMVIDPLRIRYCLEKALYLAKSGR
                     RGPSWLDIPLNVQGAYVETDELIGFDPVDYENGGNGWAEKKNVPAIPEDEAGEGEKRQ
                     VLPKKVTEEIAKAIIEKIRQAKRPVINAGNGIRLAGAHETFLKVAEKLGIPVVTGWDS
                     EDCIWDEHPLYVGRGGNMGDRPGNFAIQNSDLVLSIGSRLSIRQVGYNYKTWARAAYV
                     IVNDIDEEELKKPSVHVDMPVHADAADLLSVLDQCLDQVLEAEKDTLPDSLSEPGKKQ
                     VFTYGEGLKGMTWNETCAMWKKKYPVVQKKHWEQGEDKAANVYAAVQAISSRLKEGQI
                     TVVGNGSACVVGGHAQIIKKGQRFISNSAVASMGYDLPAAIGIWAASRKDGAYSMGAA
                     REGEDVILITGDGSIQMNLQELQTIIHHKMGIKIFIINNGGYHSIRQTQKNFFGEPLV
                     GVGYDSGDLSFPDMEKLSAAYGYPYVRAEHNGQLAEAVEKTLAMEGPVICEIIVSTDQ
                     NFEPKSSAKRLPDGTLVSPPLEDMAPFLPDEEMDENMIIPRIKG"
     gene            26881..28263
                     /locus_tag="ACZPCL_01335"
     CDS             26881..28263
                     /locus_tag="ACZPCL_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HIR1161166.1"
                     /translation="MRKQTTFLAVLSTAVFTASMAFPAYAKAAGWTEENGNWYYYDSY
                     GDPLTDTWKKSGNDWYYLNADGVRAYSEQIDEYYVNEEGKRVTYQWVTMDNEDYWSED
                     DAPEFLHYYYGKDGKALTSTWASIDGNWYYFNEDSIMETGSIQVDGYNYYLGEDGSRK
                     TGWVLLEEETDDPEDLNAWYYFDSNGRRIENEIDKKIDGSYYTFEEGKMQTGWYKLPV
                     TTENTAASDNTQAEDSAETATASQATAADQEDNSVSLPAISGYKYYDEDGKRASGWRT
                     IEGVENISEDGEYFRFYFKNGAPYYGKEDLEIFTIDSKKYAFNTAGEMQTGKQSIRLS
                     DGTAANYYFDEEGIMKTGKQTIYDEDLGETQNWLFHAEGSKKGQGYHGIKDNTLYVNG
                     LRQEADKDLRFAPVSLDDTRYLVNVNGAVQKAGSTSKSASRPELGSGYKDFKDENDMI
                     WTVNTEGIIQ"
     gene            28443..28880
                     /locus_tag="ACZPCL_01340"
     CDS             28443..28880
                     /locus_tag="ACZPCL_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161167.1"
                     /translation="MRELAVFYCPKCGYYAYYQTSRHPQCPRCCHPDVMHMARMYYKE
                     FMDMGCRERDDYLAQEIMKDNPSFLKRINAPHKRFNSREIIAEMNIEIMRLDTENKIL
                     SDTVKWMHDTIWEMIREQKGYTDQSAAQIINSLQDDKSTHTDH"
     gene            complement(28915..29751)
                     /locus_tag="ACZPCL_01345"
     CDS             complement(28915..29751)
                     /locus_tag="ACZPCL_01345"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR1161168.1"
                     /translation="MILKIKKRKKEAVRGGVVFLMAACICLGQAAPVLALDAAWSQKV
                     EITAHRGDSIKALENTIPAFEKAIESGADWIELDVKETRDNVPVVFHDSSLKRLMGQD
                     GTIREMTLEEIKKLHPAASMGPGYNNVKIPTLEETLDTCKGRIKLNIEIKEDGTESSD
                     FTERVIQLIRDKGMTDQCMITSFCYTVLEKVKAVEPSLKTGYIRSKDIEDFSQYPAAD
                     QYMLSIELIRKDRVDQIHSLGKKVTAWTVNDLYSLRKCESAQVDDMITDDPGGLLKLD
                     DQ"
     gene            29847..30161
                     /locus_tag="ACZPCL_01350"
     CDS             29847..30161
                     /locus_tag="ACZPCL_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161169.1"
                     /translation="MSINELLNDLKETYSFSSVTCSQKPDDAMTDFVFYLTQDQITKV
                     IEKASRLNSIVESCANMISICEPELKDTLMATTLRCVGANELHIRTCDSMIKMLIQSL
                     FD"
     gene            30317..31246
                     /locus_tag="ACZPCL_01355"
     CDS             30317..31246
                     /locus_tag="ACZPCL_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161170.1"
                     /translation="MQKKHFRILVAAACLCAGLSFSAYGEETISSVPLSFSWDGAPKG
                     GEAVGTLTATTTSQSFIVEGSEYLKDDDTWDYGEWPCAEIELSAAEGYRFSTPNRRTF
                     SLSGCNVQYKSSEIDSDGSTLILQVYLSRIGGQLPKITDLSWSGKNAVWDNIEGCDGY
                     EVCLKKDGKTLVTVTSKGTSYDFSDYINNAGSYVFTVRAVSTYGSQNSSWSAGSEPYI
                     LSVEDAWYSDNGNWKKTGNKWRFVYKDESYPACSWREIKEKWYYFDGNGYMVTNCYVK
                     ALGENLYYWISEDGVWDENETTATPNRARYLVY"
     gene            complement(31373..32290)
                     /locus_tag="ACZPCL_01360"
     CDS             complement(31373..32290)
                     /locus_tag="ACZPCL_01360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1161171.1"
                     /translation="MVGIVILNYNSWADTVACVDSIHEAEKKLEFRIYLVDNCSPVRP
                     EPGVLESLDKRGVIFIQNERNAGYSAGNNIGIKRALEDGCEEVLISNSDVRYETGSIS
                     ELQTYLQDHPDVGIVGPKIFLRDGSIQKECMMRKTGIREKYLLRTRFYLFFPRLNNSY
                     WGREHDYESEVFQVYAVMGCCFMFSRKCALDVTPLDETPFLYEEELILGISMEKKGWK
                     TVYDPNSVIHHLHGNSTEKVKAFAYTCNICSEIYFCRQYLNMKKWQILPLYWYRSVLY
                     GLKMLRYQDFRKYVGEYVKRTGLELKKIL"
     gene            complement(32292..33185)
                     /locus_tag="ACZPCL_01365"
     CDS             complement(32292..33185)
                     /locus_tag="ACZPCL_01365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1161172.1"
                     /translation="MAEPRYGEERKQMGSIVGDRPKISVIMGVYNGADRVDEAIASIR
                     VQTFTDWEFIICDDASTDNTWEKLVEWSKIDSRIVPIRNEKNMKLAATLNKCLEHASG
                     SYIARMDDDDYSYPWRLMAQNDFLDVNTYYAFVSGQVNGLTKEFGIIEDYWHRKEFPE
                     KKDFLKGSQFIHPATMFRKGPLMKAGGYRIAPETRRMEDYDLFMRLYAMGYIGCNLQR
                     PMLRYTVDFDKTTYAHRIDEVKVRYQGFKALGLLPWGFIYVIRPLIVGLIPHDTLMEI
                     KLEDNGVDLRKYRRRHRRRRK"
     gene            complement(33307..33786)
                     /locus_tag="ACZPCL_01370"
     CDS             complement(33307..33786)
                     /locus_tag="ACZPCL_01370"
                     /inference="COORDINATES: protein motif:HMM:NF019623.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease domain-containing protein"
                     /protein_id="HIR1161173.1"
                     /translation="MDQYQWQFSNTEELQFFWKAVEEGNTEGIEKYLNRVLSNSVSVF
                     DTKARNEEKESSYHNLLVGILTGNADWLIKSNIEAGEGFADIVAETDDPDSGIIVELK
                     YTKEFERMEQARQKAIAQIKNQRYYEYLLNDDRRNILFYGITFCKKRCKVIVERINM"
BASE COUNT         8770 a         8584 c         6602 g         9937 t
ORIGIN      
        1 ctcattactt gacttgccat aaaaaaagtc gcctcctttt cgcactttat aaaccaagta
       61 cgacaagcgg tgactgtaca cgtttccgtg tgtgtcctca cagaactaca cactgattct
      121 gccctaaatt gcagtttctt taattggtac agtgacttgt cgccagtttc ctaacttgac
      181 attcgctcca cggaaaacct gccttttgta aggcagcaac ctcacgtttc tttgctatca
      241 tgtcacagct gttctaagat aacatatatt ccagagaaat gcaagcattt ttttgtattt
      301 ttctaaataa tttttataag gggttttatt aaaaaaggaa acaggctttt taccacctgt
      361 ttccctttat tctgccctgt tctttctata attaatacat cagtaataca ttaataccgg
      421 tactgttaat ccctgcatca gcacatcctg atctgatcct atttattata gaagaaacag
      481 atatcatttt atcagtcctc tgtacagatc ggttcttccg gctctgtgaa ataagagctt
      541 acatcgttat catcatctgc tgtacttaaa gagccgctgg agcttgtttt aaacttcact
      601 ccgtcactgg tctttacagt agagtttttc ataacctttc cggaagaatt tactacatag
      661 ttgctgctgt cgatcttata gcatacatac ttctgaccat ctgtagcttt ctgaagcctt
      721 cctttataat agaggtaatt atcttttaca ccggtatagc cgcggccgtt ttcactgaag
      781 tagaaatctg tcttgtttcc atcatcttct tctactttca tctttccacg ctgtacacag
      841 ctctgtgtct tggttccaaa atagaacgcg gtccagttgc tgtcaccgga tccgatatag
      901 accttcttaa gtccatagac cggattgcca cgcttattaa ataagtaggt ctttccactg
      961 atcttggtca gatcctgtgt ggtaaggtga tctgagtctg cagcttttgg ctttccatta
     1021 tttgcaaagt agaaccattc tacatctcca tcataacctt ctaagtcttc cggcggttct
     1081 atggaatacc aaccgatctt tgcctttccg tcagagccaa agtacatata gtctttgatc
     1141 gagtcagaac ttcctcttac attcttccag cccatctgta aaatgccgtc atcatcaaag
     1201 caatagtaaa tgccatcgat ctttctggtg ccataatctc cgttgtcgtt gccgtcactt
     1261 ggaacatatt tcttgccgtt tgttccaaaa tagtaccaat actggccatc ttcgccgtaa
     1321 tctaatgtac cgccctcagc tcccgggatc actttgttgg agtcttcctc gtaattatcc
     1381 ggcggataca gtttttgcca gccggtacgc atcacaccgt catctccggt ataatacata
     1441 tcgtctaaga tccagcctgt ttccatacgg ccgtttccgt caaaatgata ccacttatca
     1501 ttgatcttct tccaggtgtc atctatggtc tttccgctgc ttccaaaata gtaccactct
     1561 tcttctccgc cgtcagaagt agtctttaac catttgtccg taaccataat gccattgctg
     1621 tctacataat aatcagagtc tgcccatgca tttacagcca tatatccttc tccgttcaga
     1681 tatctccact ggccgtcagc tccctttttc cattcattat atactttatt tccattgctg
     1741 tcatagtaga cccagctgct gttttcctgt gcccatccct gtgctgccat agccgggaac
     1801 gctgccagcc ctgctgatag taacgctgcc agaaaccagg ctgctttcat ttttttcctc
     1861 atatgccctg ttcctctctt cttctgcttt caccccgaaa ctgctgtaaa tatacagttt
     1921 gcgctttata actattgtac caagagtttt cttatagttc aaccagatta atctttcaat
     1981 tctattacaa gaatatgtca ctggtactca aatctcttga cggaccctat taaaatagac
     2041 tatactcaat tggagtccta tgatacaaaa ccgtactata acatccgccc tgggcggaat
     2101 acaggatatc atatatatta taaatagaag aaacgaggtt atcaaattta tgtggcttgc
     2161 cgacggttgg aaagattatg aagtaattga ctgttccaaa ggtgaaaaat tagagcgctg
     2221 gggagattat cttctggtgc gtccggatcc tcaggtgatc tgggataccc caaagaaaca
     2281 caaaggctgg aagaagatga acggacatta tcatcgcagc agcaaaggcg gcggtgagtg
     2341 ggaatttttt gatctgccgg agcagtggac gatcaattat aaagaactta cctttaattt
     2401 aaagcctttc agttttaagc ataccggtct tttcccggaa caggctgcaa actgggactg
     2461 gtttggtgat aagattcgca aagcaggacg ccctgtaaag gtattaaacc tgtttgccta
     2521 taccggaggt gctacactgg cagctgccga agcaggagcc agcgtgaccc acgtagatgc
     2581 ttctaagggt atggttacct gggcaaagga aaatgcccat tcttccggcc tgtctgatgc
     2641 tccgatccgc tggatcgtag atgactgtgt caaattcgta gagcgcgaga tccgccgtgg
     2701 aaatcactac gatgccatta tcatggatcc tccatcttat ggccgcggac caaagggcga
     2761 gatctggaag atcgaagatg ccattcaccc attagtaaag ctgtgtgtac agctgctttc
     2821 tgatgatccg ctgttcttct tgatcaactc ttataccacc ggtcttgcgc catctgttct
     2881 ttcctatatg atgggcgttg agatcgtacc aaaattcggc ggaactgtaa aagcggagga
     2941 agtaggtctt cctgtttccc agaacggtct ggtgcttccg tgtggcgctt ccggacgttg
     3001 ggagaaataa aatacgatat tctttgcagg ctgctgacag atgataagat catatttcat
     3061 ctgccagcag tctttttacg tccagttttc cccagccctg ctggttatgg ggaagtccca
     3121 gatcgactgc ccgctccctg atccgcagtt taacgtcttt attactcata gacggatatt
     3181 tttccaaaag cagggcaata gcaccggata caagaggcgt agacatgctt gtacctgatt
     3241 ttataaaata ccggcccggt tcattacagc agctgatgat cccgcagccc ggtgctacca
     3301 gatctggctt gcacacacag gcattggtag gacctctgcc ggaataatcc accattctgc
     3361 ttcccatcac ctctacctcc cggtcatcat cagaacagcc tactgtgatc actttccggc
     3421 tgatccccgg cgtagtcaca gttcccggag caggaccatg atttcctgca gccaccacca
     3481 ctaccaagcc gtcatcccag gcagcattta ccccttttac caaggcggaa ttttccgtca
     3541 tttcccgtct ggtaagggat cctacagaaa tatttatgat ccgtatgcca tattctttcc
     3601 tgtgttcccg gatccaccta agtcccatta aaacatctgc cgcatatcca tttcctctgt
     3661 gatctaaaac cttgatcgct gtaatactgc ttcccggtgc gatccccttt aaaaggccgc
     3721 ctgaggcaag accgctgcct gcgatcatag ctgcaatatg ggttccatga ccattatcat
     3781 cataaggaag cttttttcca tggacaaagt ccttaaatcc ctgtatcttt cctttcaggt
     3841 cttcatgaag gtagatgcct gtatctaaaa ctgcaacacc gacgcccttt ccggtaattc
     3901 cctttaaagc cgcctcatca cagtgaattt ccattcttgc ccgattcaca attaaaaaat
     3961 cccttttctt atactgtatg cgcagaaaaa agctccgtac ctgtcagtac ggagctttct
     4021 tctaaggatt tcggattttc aaaaagggtt gaaggatagt tcttaaactt tcgtgcattt
     4081 cttatgtaac taaagtatat catgcaatcg ccggaaatgt ttgaatgttc tttgaataga
     4141 ttattaagaa agtcttaaga ttgcagtaag agtcccgtac agcgactgag gatacaggca
     4201 agcttacttg catggataca cagtcattgg acgggctgag gcgaaatgag cacgtgcgta
     4261 gcaatccact cttactatga attacgaaag gacagattcg taaagtgtgc gttttaactt
     4321 ttccacatcc ccgtctgcaa agcagtaacc gtccataccg caggctttcg ctcctgcaat
     4381 attatttggc aggtcatcaa taaaatagca ctcttccggt ttcagaccga accgttcaaa
     4441 gaaatgacca tacatttctt tctgtggttt catgcacaga acttctgcgg aaaacaggat
     4501 cccgtcaaag cattctactg ccggcagcac ctctttgtag caggtaagca tacgtacaga
     4561 tgcattggag cataaataga tgccgtagcc ttttgctttc agatcccgga ccagatctcc
     4621 catctccggc ttctggacca gattatactc atgccagtgg tcgaaagact tcacagccag
     4681 ttctttttct tcttctgtat ccagacggct gatcatcttc ttaataccat cttcctctgt
     4741 gatcactccc atatccaaaa gaacccattc cggtgaatca aacacagccg tgcagactct
     4801 ctttgccagt gcctcattgt ctgtaaaatg acggcatacc ggcataccag aataatccac
     4861 cagaactttt cccatatcaa atacaatatt cctgatcaaa ataaccccgc ctttcttatt
     4921 atgcaaaaat gccggacgtt ctcacctgtg cagaccggct gccaagctcc aaacctccga
     4981 catttttaat gttcattttc tttgtaaaaa accagtctta ttttgtctct ggcttctcta
     5041 cctgaacgat cgcagctttc tgcattggga tacggcagtt tttgttgctg ccgaactcaa
     5101 cgatcacggt atcatcggtc atatcaatga tcacaccgta aaaaccgctg gtagtaagaa
     5161 tggtatctcc tacagcaacg ctgcttaaca tctcctgctg tttctgtctt tccttcttct
     5221 gtggacggat tgaagtaaaa tagatcagag ctgcaaaaaa tacgatatag ataactataa
     5281 agcccattcc ctgcatggtt tttcctcctt cacatctgct gccgcctcgc cgcagcagtt
     5341 atagtttttt gtttacccgc ttctacctgc tatttgcctt ccataccggc aagtttagct
     5401 gccttgtatt ctgcgaaacg gtgttcttcg atggcgtcgc ggatctcctc catcatttta
     5461 ttataaaaat acaaattatg caaggtacat aaccgcattc ccagcatttc ttttgccttt
     5521 aacagatgac ggatataggc tctgctgtag gaacggcagg ccggacactg gcagccttct
     5581 tcaatcgggc tgtggtccag ttcatatttc tggttaaaca gatttaattt gccatggttg
     5641 gtgtatacat gaccatgacg gccgtttctg gatgggtaaa cacagtcaaa gaaatctacc
     5701 ccacgctcta ctgcctctaa gatattggca ggtgttccta cccccataag atatacaggc
     5761 ttatcttccg gcagatgagg taccgtcaca tccaggatgt gatacatttc ctcatgtgtc
     5821 tcacctacag ccagaccacc gatagcatag ccgtccaggt ccagctcttt aatggtttct
     5881 gcatgctgga tacggatctc atctacaaca ccgccctggt tgatgccaaa caacagctgt
     5941 tctttgttaa tggtgtccgg aagggaattc aaacgatcca tttctgtctt acagcgcttt
     6001 aaccagcgtg tagtgcggtc tacggaattg cggatataat cataatctgc atggcttggc
     6061 gggcactcgt caaaagccat ggcaatggtg gaaccaagat ttgactggat ctgcatactt
     6121 tcttccggtc ccatgaagat cttatgtccg tcaatgtggg agcggaagta tacgccttct
     6181 tccttgatct tgcgcagatc tgtaagggaa aatacctgga aaccgccgga gtctgtaagg
     6241 atcggcttat cccagttcat aaactgatgc agtccgccat actccttgat cagcttgtcg
     6301 ccggtacgca catgaaggtg gtaggtattg gaaagttcta cctgagtacc gatctggcgc
     6361 aggtcatcgg tagatacagc gcctttaata gcgcctacag ttcctacatt catgaaaacc
     6421 ggtgtctgga tggttccatg tacagtttcc atctgggcac gtttcgcccg tccatctttg
     6481 gttattacct gatatttcat atattatgcc tcttcgtctg ccaaagcagc cttgatcatg
     6541 atggcacatt cgatcctctt tttctgcatc tcgcagccga tctcatatgc gccgttaata
     6601 gaaccactga aattataggc atttgcacta catccaccgc tgcagtaaaa tcttgcaaag
     6661 cagtccttgc atgtctcttt tgcatataca ttgcaaagct taaactcatc acgggtctct
     6721 gtatttacaa tacctgtgtc tacatttcca agaaggaatt tttcattgcc tacaaactgg
     6781 tggcatggat acagatcgcc ccatggggta actgccagat actcagtacc ggaaccgcag
     6841 ccagccagac gttttgcaac acatggacct gctgttaaat ccagcataaa gtggaagaag
     6901 ttaaaacctc tgccctcttt tttgcgcttg atgtattcta atgccagttt gtcatattct
     6961 tttaagatct gaggaatgtc ctcttcacgg atggcataat cctctgcagg atcagctact
     7021 accggctcca tggacatctg ctggaagcca agatctgcat aatggatcac atcatctgca
     7081 aaatccaggt tatttcttgt gaaagtacct cttacatagt aattcttctg tcctctgctc
     7141 tcagcaaact tctggaactt cggaacgatc atatcatagc tgccgctgcc gtttctgaac
     7201 ggacgcatct tgtcattgat ctcacggcgg ccatccaggc ttaatactac attgctcatc
     7261 tcacggttgc agaattccat gatctcatcg tttaagagaa cgccgttggt ggttaatgta
     7321 aaacgaaact tcttgtggtt tgcctcttct aaggagcggc cgtattcaac cagctgtttt
     7381 actaccttcc agtttaaaag aggctctccg ccgaagaaat ccacttccag atgctctctg
     7441 tttccggaat tagctaccag gaaatccagt gctttcttac caacttcata gctcattaaa
     7501 gcacggcggc catgatactc tccttcttcc gcaaagcagt aacgacaggc aaggttgcag
     7561 tcgtgtgcaa tatgcagaca gagcgctttt acaacggtct tgcgcttctt aaaatctaaa
     7621 acaaagtcac gataaatgtc acgggtaaga agctgttctg tatcgatcag ctcctgaata
     7681 tcttccagtg cttcacagac catttcactt ccataggtct cgtctaattt tttatgaaca
     7741 tttgcagtta cttcttcaga aagcttctca ggcttgtcca tttccggaac ctgttctgaa
     7801 actgctgcga tggcgtcata aaccaccgga tctactacat ggacggaacc gctgttcaca
     7861 tccagaacaa tatgataacc attattaata tactgatgaa tcaaaattaa aatctctctt
     7921 tctaaacgta ataggccttt caggccaatc caaatcacat gggaaagccc ctataatctc
     7981 tgtgaccata ggggctttgc acacttaatc cgttaccgac ctataccatt agcgggcaga
     8041 gttttcgcag ctctggttac caacggtaca ggaagtctta catgcagact ggcaggaagt
     8101 ctggcactcg ccacatccgc ccttcttcat ggtatccttt aaggtattgg catttaaagt
     8161 ctttacatgc ttcattgtac agtacctcct ttttttatat gcctcaaaag gcacatatga
     8221 ataaactcca taaaattata acataggtta tgttggcttg caatggtgaa tatacttgac
     8281 tgtaaacata ttttagggag atgtctatgg aaactaaagt gataaaaccg ctgctgcgct
     8341 ctctttttat ctcctatgcc ctttctgccc tctttttagg ggcactggct tttgcccttt
     8401 acaaactgcg cctgggagaa agtctggtaa acctgctgat ctttgctgtg tattttgcag
     8461 cctgtttcgc aggtggattt ttcacaggaa gaaaagtcag aaaccggcgt tttttctggg
     8521 gactgctgtc aggaatgttt tattttctca tcctttttgc tgcttcctgg gctatggatc
     8581 cccgggcctc tatccaggct gccagatctc tcatggtatt aggcgtgtgt gcctttggcg
     8641 gaacccttgg gggaatgctc agtttttaag gtctatatcc tccaacagtt ccccgatcag
     8701 aaataccagg ctgcctaaaa atacaaaaat atctcccaga ttaaagatca cctttttcag
     8761 ccttttaaat tcaatggaga aataatccac tacatagcct tttaccagac ggtcataaag
     8821 attgctgata gcacctccaa ggaccatgga aaggccgatc ttctgggcca ggcagccctt
     8881 ttttgtcata aacgcgccta atgcgcctgc tgcagcagat gtaactgcca aggggatccc
     8941 ttttaccagt tccgggcgtt cttttaaaaa gccaaaagga aatccggaat tataactttt
     9001 atgaaggcgt atcagcccct ttgtgccggg aaggtccctt ggaaattctt cttccccttc
     9061 ccgctctatt tcgtctttta taaacagatc cagggcagca atgccgccag taagccacgg
     9121 taagatcata ttatacatcc atcctttctg gtacatggaa caggttcgta cttttttcat
     9181 gagtccattt tacaatatta tcccgtttcc gtccagatat gcaggtaact tccggtgatt
     9241 ttcccatata ctgataacaa gcattttacc ggtggttttt atggctgaat gtatcttaaa
     9301 tcctgcttcc caggatgtcg ctgatttcat ttaccgtttt ggatccctgc agaataatct
     9361 ccgggacccg gagcagtttc cctgtgtgga ttatgtaagc aatgattatg ctgtttttta
     9421 tacagagctt gaaaacgccc ttcccctttc tctggaacgt cagccctact acagcattcc
     9481 ctctcttttt accctgttgg acgactccgc catggaagct gccgggatcg tttccgccct
     9541 tcagacacct gcactgagca atatgggaag cggggtgatc atcggtattg tagatacagg
     9601 gatcgactat acaagtcctg tttttcgtaa aagtgacgga acgacccgga tccttggtct
     9661 gtgggatcag acccttcctg aggatccttc tgttttaccg cccggtgtac cggaatatta
     9721 tcccatgggc ggtgcctcct atggaacgga attcactcat gaagagatca acgaagcttt
     9781 aaccttagag gatccttttt ccctggtacc ttccaaagat acagatggcc acggcacttt
     9841 cctggcaggt cttgcagccg gaactgcttt ccctctccag aacttcacag gcgctgctcc
     9901 catggcagat cttgccattg taaaattaaa gcctgcaaag aaatatctgc gggactatta
     9961 tctgatttcc gaagataccc ctgctttcca ggaaaatgac attatgatgg gaattaaata
    10021 cctgcgggtc atcgctgacc ggttcagacg ccccttagtc acccttttag gacttggaac
    10081 caattacggc agccataccg gtacctcccc cttaagccag gtaacgcaga attatggcgg
    10141 ctttttcggg atcgccactg taatcgcagc aggaaatgaa acaggactcg cccaccatta
    10201 cgcaggaagg ttttccgctg acacttcttt tgaagatgtg gaacttcggg taggagaaga
    10261 agaagggaaa cgtggcttta tcctggaact gtggtcttct gctgccgatc tgtatacagt
    10321 aggctttgta tctcccggcg gagaacggat cagccgtatc cccattttat ccaataatga
    10381 gacccgtatc ccttttcttt tagagtctac ggtcatcacg gtaagttatc agctgatcga
    10441 ggcaggaagc ggaagccagc ttgtatccat gcgctttgag cgtccttccc cggggatctg
    10501 gaccattcgt gtatacaata cacagtttct tacgggagaa taccacatgt ggctgccggt
    10561 tcagggtttt atctccgatg aaactgtatt tttaaaaccg gatccttcaa acacgatcac
    10621 ggttcccggc aattcccgcc tgcctattac aacaggggcc tacaatcaca gaaacaacag
    10681 tatctacatc cattccagcc gtggctacac cagcagagat tatgtaaagc cggatctggc
    10741 tgctccggga gtggagatcc tggggccatc ggtgcggatc tcagctgctt ttcgccctgg
    10801 gctttcatcc agtgtagcag ccggtactgc tcctgccttc tcccgccgca ccggtaccag
    10861 cgctgcagcc gccatcactg caggagcagt tgccaatctt ttaagctggg ggatcgttat
    10921 gggaaactac cctactatga gcgaggcttc tattaaaagt tatctgatcc ggggcgcagc
    10981 cagaaatccg tcccttacct atcctaataa agaatttggg tatggaaccc tggatctgct
    11041 gcagacattt ttacggctgc gtgagtgatc gaattccata cccaattcat tttatccttc
    11101 tcttacctgg atcaggggac ctcccttttt ctccagataa ggccttgtga ccaccacaga
    11161 cccgatattc gggtcttttg gtatctcata catgatatca agcataaact cttccagaat
    11221 ggctctgagg gctcttgcgc ctgttccacg ctttaaggct tcctctgcta tccaggaaag
    11281 tgcgtcttct tcaaatacaa gttttacctc atccagtgcc agaagttttt catactgacg
    11341 caggatggca tttttcggtt cctttaagat ccggatcatc atatccttat ccagtccctt
    11401 taaggtaaca tttacaggca gacgtcctaa aaattccggg atcattccaa atgccctcag
    11461 atcttcattg gtcacatggc ttaaaagatc cggatcattg tcatagctgt cttttaaggc
    11521 agctccaaat cccatggagg atttctttgt aaggcgctcc ttgatgatat tttccagatc
    11581 cgggaaagca cctccacaga taaacaggat attatctgta tttacagtag ccataggagt
    11641 cattgcattt ttctgattgg atcccaccgg aacttctacg gtactgcctt ctaacagctt
    11701 taaaagttcc tgctgaacgg actcgccgct tacatccctt gtattggtct gttttttctt
    11761 tgcgatctta tcgatctcat caataaagat aatgcctttt tctgcccgtt ttacatcatt
    11821 tcctgcagct gctaacagct tggaaacaac gctttcaata tcatcgccaa tataaccggc
    11881 ttcagtcagg gatgtggcat ctgcaatagc taacggcaca tcaagaagct ttgccagcgt
    11941 ctttaccaga taggtcttgc cgcttcctgt agggccgatc attaaaatgt tggatttttc
    12001 gatcacaaca ccgtctgggt cggaaccacc taaaaaggcg cgcttataat gattataaac
    12061 agctactgcc atgaccttct ttgccttgtc ctggccgatc acatattcat ccagcttgcc
    12121 ttttataacg tgaggcgccg gaatatcttt catagtcagc tgcttttccg gttctttttt
    12181 ctttttcagt ttctggctct gagggatctc attcactggc ggcatctggc ttaagtcact
    12241 gaaattcatg ttcatataag gcatattggg catttttgaa aaatccatgc ctcccttagt
    12301 cactgtatca aaagccttct gcatacagtc atggcacaga cacatgcctc ccggcattga
    12361 gatcattggt cctgccttgc tctccggtct gcggcacata tagcagatct tctcataatt
    12421 atcgttattt ttattatcat cctgggatcc atttgtcatc ccgttttcca tattcttatc
    12481 gtccactgtc ttttctcctt accacattct tccccatggg aagccatttc ccggaaaact
    12541 ccagggatta ctgtcttctc ttctggaagg tgctgtttcc ccgggctgct ccctgttatc
    12601 ctgtttctcc tgttgtcctt caccttccgg cagtcctgcc agcgtaatct ggatctgctg
    12661 ttccttatac cggccttctt cctggcgctg caccagcagt tcaatggtct ctcccatttc
    12721 atagcctttc aggaatttct gcagttcctg catactcttt acagtcatgc catccagctt
    12781 ggtgataata tctttctcac gaagttcaga gccagccgca gccccgtctt ccagtatctt
    12841 gtatacataa acgcccttcg gcatatcaaa ggttgccgct gtttcttcat ctactgtagt
    12901 tccctgaatc cccagatatc cccgtctgct ttcctctact tcagaacggg tcttacggtt
    12961 catcagttca tctaaaatct cctggactcc tgaaacagga atggcatagc caatcccttc
    13021 tacttctgtg gaggaatatt tagccgcatt gatgccgatc acttcccctt tcatgttcaa
    13081 aagggcgcca ccactgtttc ccggattgat ggctgcatct gtctgaagca ggactcttgt
    13141 atttccgtcg gaagtctgta cttcccggtt taaggcgctt acatatccca ctgtaacgga
    13201 ctggccatac ccaagagcat ttccaatggc gatcacgccc tgtcctactt ttaagctgtc
    13261 tgagtcccca agccttgcaa cggctatctt atcctgggtt tcctgtggga tctgatctat
    13321 cgggatggaa ataactgcca gatctgtatc tgcatctgtc ccctttaccg cagcatccac
    13381 agctgtatca tctacaaatg tgatctttaa tgatactgtg tccttcacta catgattatt
    13441 ggtcacgatc agcagttccg tatcattttt accaatgatg atcccggaac cactgcttgg
    13501 cacctcataa gactggcttc cgcctccgaa aaaccagctg tatccgtaat ccggagtctg
    13561 gtaaacggct gtgctggcaa ctgccacaac cgttggcatt gcctcttcta cgatctctga
    13621 aacatccgtt gacggtgccg gaatgtctgc tgcctgtgga aatgtttccg gtatcttttc
    13681 tcccggaaat ggttctggag gcggcagtgc ttctccctcc gcctttactg tatcctcatc
    13741 tgacgcaacg atcgcaaaaa actgggaaac cattccggaa gacgcaatct ggatcccggt
    13801 cataaccgtt cctgccacgg ttccaaaaag gactgcggaa agaacgatcc aggctgtctt
    13861 tttcaacaaa ttgtttttcc tcatatatga tctgttctcc ttccaaaaag agatcccgca
    13921 aaagaggaat tctccctgct ttcacgaaat ctctttctta aactttggtg ttatctttta
    13981 atgcttatta agctgccggt gagatcatct ctcccggacc gcctacattc tcttctttga
    14041 tgctgtttcc aggattgaag gatgaagctg ctgttgtttc actgcttccc ggtccatatg
    14101 gttttgtagc agcctctgta gttgccgctt ctgtggtacg ggaaggtgac agcgtaccgc
    14161 ttggaaccgt aggctttaag ctttctgtgg agtctcccgg atgggctgcc tttgtagtct
    14221 ctcccggatg ggcctgactg ctttcacctg tatgagaact ctgggtcgtt tcagaagaat
    14281 gtttactggt tggtggtata taatcttccg gaggatcgat cacatttcca tcttcatctg
    14341 tttcatactc aaaatcagtt tcaccgtcaa taatactctc ttttgtggaa gaagtacttt
    14401 cattgttctt ttcagaagaa gaactgcttt ctgagccgtc cgctgttgtc ttgtcttctt
    14461 tctttgcctc tgtagtctta ggaatataac cgccatctgt accaacatca cctaccggct
    14521 ttccttcttt atacagacct gtatcagaag caatctttgc gctgatcttc tttaccatat
    14581 cacttggggt gtaattttcg tcaccaaaca ggaacttgtg aagagaaacc acattgctct
    14641 ctaaggtctg cggaatgaca cagtcaccct ttttacccat attggcatca cttcttgcct
    14701 cagggaagcc ggcggtatca ccgatatgat aacgggtcag gttctttgca gccgggatca
    14761 catcatctaa tgtgatactg cttaagatct gtgggaatac cacttccatt acattattaa
    14821 caacagaaaa gtttgcctgt cttaatttat caaagcttaa ctggatgatc tttctctgac
    14881 gttctgttct tgcaaagtct gtatccatct ttcttaagcg tgcataggca actgcctgga
    14941 taccgtctaa atggttcggg cctgcgctct ttaactgata agagccaaca cctgtagact
    15001 ctactgtttc tgtaatatag gcattgatat aataaaactc cgccttgctc agttccacat
    15061 ctacaccgcc caggatatta atggcatctg ctactgcttt ccagttaaat gtagcataat
    15121 cattgatctc cagatccagg tttctgttta aggcttctac tgcctgtttc gggccgcctc
    15181 tgaagtaagc ctggttgatc ttgttgtaac catttttatc atctacattc agatacgtat
    15241 cacggaaaag gctgaccagt ttgatctccc ctgtttcctg attgatattg cagatgatga
    15301 taacatcgga catatttcct ttgcccacag aactgtcacg gctgtccaca ccaaaaagag
    15361 ctacggtcca gtatcccttc atagcctgct ctttttccac agagatataa ggatttgtca
    15421 tatcagactt attaaactct gtatccgtct gcatcaggga catcatacgc tttccatacc
    15481 agcctgcgcc gatcagtgcc agtgcaatga tctctgccac aataaaaatg ataatggtcc
    15541 ttaagcggct tttcccggac ctggctgccg tccttgcagt ccggctgcct cttcctgtac
    15601 tgcctgttct gccggtaagt ccggaagaag cgtatttcct tccgtctttt ctgcggctgc
    15661 gccctctttt taactcgtca tccgtttcaa attcattatc aaagtcgtga ttcatgtacg
    15721 tttcctcatt gtagtgataa cagcagtcag accgctgtca ttctagtatg cgtttttatt
    15781 aataaagcct ttgaatatcg tcaggaacat gatcttaaaa tcaaatccaa ggctccagtt
    15841 ttcaatgtaa tacaggtcgt gctcgatcct ctttgtaata gaagtatctc ccctgtaacc
    15901 attgacctgc gcccagcctg taagacctgg tcttacctgg tgcttgatca tataacgtgg
    15961 gatctcttct ttaaactttt ctacaaaaag aggccgctcc ggccttggac caaccaggct
    16021 catatctcca atgagaatgt taaaaaactg aggcatctca tcaatactgg tctttcgtat
    16081 aaaacggcct accggagtaa cacggggatc atgaggggtt gtccactggt tcttttcttt
    16141 atttggatcc tgtacttcca tggaacggaa tttaaacatt ttaaaaggac ggttgtgcag
    16201 tccgatcctc tcctggctgt aaatgatcgg acctggagaa gtaagtttga tcaatgctgc
    16261 tgtcagcagc ataagaggcg aaaacaggat gattccaaac agtgcgccac agatatctac
    16321 catacgcttt atagttgcat taaatacgct ggtaagaggt acatggcgga tatggatcac
    16381 cggaagtccc tgaagatctt ccatataagg gatcgtaggg ataatgttat tgtaatccgg
    16441 aataaatttg gtatgcacac ctgacttttc acaggccgca acgatctgtt ccagatttgc
    16501 atattcaccg atacttaaag taataacgat ctcgtctaag gtattcgctg caagaaaggt
    16561 ctccaaatgg gagatcggtc ctaaaacctg gactttcttg taaacataac ctgctttttt
    16621 atggtcatcc aggataccct ggatatgata gccccattcc ggatttaaag tcacacggtc
    16681 aataaaacct tctgccgcac ggctgtaacc gattaagagc acatgcttct ggttgtaacc
    16741 attggtacga agagaacgta agaacaggcg gatcgccagt cgttctcctg ccagaagaag
    16801 tatatttaaa acaaaaaaca ccaggagcat tctggtggag aaatacccca gatgtacaat
    16861 aaagcttctt ccgccgaaaa ggatcagcgt aaaaagcatc aggccgatca catttgcctt
    16921 acagatattg gcaaattcaa cccttcttcc ctggatgcgc tttggtacat acagatgaaa
    16981 acagccattt aagatcagat aaacaggaac aataaatata agtgccagaa aataaggtcc
    17041 cggctccaga gttgctcctc ttaaaggcac tacctttccg ctgacgataa taaaccacgc
    17101 cagtatatag gctactgctg ttaccactgc atccagaaga acctgaacat ggttcaggct
    17161 tttctggtta tcttttatca ttgtatatca ccttttgtta agctgttcac tcattttccc
    17221 ccgccggaga aaacggcttc ttcattcaaa acattataca tgatacaatc ccattattcc
    17281 ataacttttc tcttaaaata taataaactt caccgttttg ccgcctgacg ggccagaaat
    17341 tcccggatat agaggagagt tccggtgcat aattcccact caatggcagc ataatgtttc
    17401 aggtggtctc tgttatattt tactttttta cacagctttg ctgtcttcac tccctctttc
    17461 agaccgtcca gataatcttt tccaaacccg atcttcttaa aaaatccata ttttaccagg
    17521 atccccagtc ccagcggaag agcatttaaa agcaactggg gccatggcat atttttatag
    17581 ttcagataaa tattgtttct tgctgccaga cggaccttaa atgaattgta acgggatcca
    17641 ctggtaccac ttcccacgtg gtataccatg gcgcttgggc agtaaacatt tttataacca
    17701 taaatcttcg ccctgtaacc cacatcaata tcttccagat aagcaaaatg catctcgtca
    17761 aaataaccga tggtctcaaa cacttccctg cgatagatgg cagcacctgc acaggcagaa
    17821 aaaacagggc agctgcggtt gtatcctgta gccggacgtc caacgccccg ttggaatgcc
    17881 cagcctaaca gtgtatacat atctcctgca tcatccattt tgctgtggtc atacagctgg
    17941 atcatcttgc tgcttacaga aaagatctcc ggtgaacgtt caatggcctt gatcatctct
    18001 ccaatatagt ggcagtccgg ttctgtatca ttgtttaaaa ggatcacata aggggtcttt
    18061 gaggctttga tccccacatt tacagcccct gaaaaaccgg tattcttctc aaggaagatg
    18121 cttggtatct catgttcctt cagccattct acgcttccgt ctgtagaacc attatccact
    18181 accaggatat caaaatcacg gcaaagctgc agatccagag ccttaaaaca gggatccata
    18241 aacttaagac cattataatt gggaatgacc accgttactt tcgccttatt actcatcact
    18301 ctttttctcc tcaggtgcaa acttatcata aactgacagg tcgtaaggtt caccgatctt
    18361 ttcttttaac agatcacgca gggcaaaatt ggactttctt aacgtcaggt tcgggttgta
    18421 gtaaggatca ccatttttaa tgatgtccgg ccatttctta atgaaaatag ctacttccct
    18481 gttaaaccgc gccaccttct caggcgtatc ttccagtccc ctggatttgg actcataatg
    18541 gtaaagagtt gcataagggg catatactac cagttttccc agagcgcgga ctttcatgca
    18601 gtaatcaata tcattaaagg caactgccag ttccgtactt aaacctccca cagcctcaaa
    18661 cacagacttc tttgtcataa ggcaggcagc tgtaacagca ctgtaatcct gggcacacat
    18721 tgcacggtgg aaataactgt tttccgcttt gtgaagaccg ataaaggtat gtccagcaat
    18781 accgccgaaa ccaataacaa cacctgcatg ctgaatggta tcatctgcat acagaagcct
    18841 tgctcccgca ataccaacct cttctctctg gcagaaccca agcatttcac gaatgctgtc
    18901 aggctctaac agttccgtat cattattaag aagcagcagg tactctccct ttgcaaagga
    18961 tgcgccaaaa ttgttgatgg cagaataatt aaagccttct ttccaggtaa ctacatggaa
    19021 ggacggatgt ttttcttcca tctttttata ataagcaaag gtctccggct ctgtactgtt
    19081 attttccaca atgataaatt ccaggttccg gtaagtagac ttctctaaaa tagaccggac
    19141 acacacatcc agatccttgc tgtgatcctt atttggaata ataatggaga tcaggggctc
    19201 tcctttgatc ttaaaggtag tatggtaaat accgtaatct acgccctttt ccacacgttc
    19261 tgccgggatg cccatacgct cataatgggc catgatcgct ctggaaccag cctcaaaagc
    19321 atacatcttg ctttccggat tgctggcagt agagtcctga tggcagcgcc agtgataaag
    19381 tacctgtggg atatggacga tctcttttgc ctgctcagtg cagcggaaga taaagtcata
    19441 atcctgggct ccgtcaaatt cctggcggaa gccgcctacc tgatctaata actctttttt
    19501 caccacgaac aaatggcaga tataattcac actggtaagc aggtccgggt taaaatccgg
    19561 cttaaaatga ggatcaaata aagcctttcc atccatatcc agcttgtctt catctgaata
    19621 cagcacatca cagtccggat gcttattgat agcctttgcc acctcataaa atgcctgagg
    19681 cggaatggta tcatcatggt ctgccaggat cacatattcc ccctttgcca tagcaagagc
    19741 tgcattggta tttccggaaa tgcccctgtt tccccccaag atctcataac ggatccggga
    19801 gtctttctgt gcatatttct ttaatacctt ttccacatcc tggcctctgg ggcttccatc
    19861 ggcaatacat acttcccagt ttccataagt ctggtccaga atggagtcta acatctcttt
    19921 cagatagcgc tccggtgttt tgtataccgg tatgactacg gaaaataaag gttctttttc
    19981 aaaatgtgtt tccctctggg ccttcagttc ttcctctgtc ggcttggtca gctcatacca
    20041 ctcaccataa tcgtaatcat tatccagtcc ctggatctta tggcaggatt ttttaataag
    20101 ggcacggaga ccattttcct tccagaaatc aagagctaca tggaccgttt ccatgttcat
    20161 cagatctttg atcttttcca tgcgcttgtg ggcaacactg gctctcttgg tgatcagttc
    20221 ttcattatat ttgatctttg cctgtctgcc atcacagcgg atcagcagcc agtaactttt
    20281 tcctctctca tatggaaact ggatatcaaa gccaaattcc ttatcgatca ctttcttaaa
    20341 atagatctgg cttacatcat ctcttctggt agaaaccatt ttgcacttca ccggctggtg
    20401 atgttcatct tctacccgga aagtgacctt gctctccggg gtctttccca gagcccatcc
    20461 gttcagtgta atggaatttt cacggatacg gaccacatcc actttatact tcatttgcct
    20521 taccgtcctt tacattttca tctttctggt caaagttcag ccgttcttct tcgatcacaa
    20581 gaggacgctt catcacgcgc tgattgatac tcataatgta ctctcccaga aagccgataa
    20641 agaataactg tacagagcct aaaaacaaca ttccaatgag cataggcgcc atacctgcag
    20701 gaaaacgatc ccaccagatc agcttcatca ccagatagat cagggcaatg atcatacttc
    20761 ctgctgcaca gacaccgccg aagatcgttg ccaggcgcag tccctctttt gtataagaag
    20821 taacgcttaa catggcagcg tcatacagac ggtagaaatt gttgtgtgtc tttcctgcac
    20881 ggcgctttgg ctgctcataa gggatctctt tgcgcttata gccaagttcc gctacgatac
    20941 cccgcaaaaa cggtgtggga tcatctaact tgcgcaggat ctcaataaat tccctgtcat
    21001 acagaccgga acctgtaaaa tgttcgatct gttctacatc agacattttc ttaatggtct
    21061 tgtagtagca ggtgcgcagc cagtacataa acttattttc cttgctactg gtcttaatgc
    21121 cgataacgat cttatagccc ttttcccatt catggacata cttggggatc atctcaaccg
    21181 gatcctggaa atctgctacc atctcaataa cgcagtctcc tgttacctgg agcatgccat
    21241 aatacggaga gttaaactga ccgaaatttc ttgcattaaa aatagccttg atccttggat
    21301 tttccttaca cagtccacgg atcacatccc gtgttccgtc tgtggagtca ttatcaataa
    21361 atacaagttc ataatcatac tgaggcagtt ccttctctaa aatatcggtt actgccttgc
    21421 tgatggggcc tgcattttcc acctcgttat aacagggaat caatacactg atctttttca
    21481 taggtttttg ttctctttct cttttttcat cataatttca atgccctgac ggaaggtaaa
    21541 tctttcctgc cagccactgg taagcagtgt aagacgtgaa atatccggct taatagaaag
    21601 ggcaccttct tttgcctgga caaaagtccc gtactccaat tcaccggcac cacctgcaat
    21661 ggaatggatc tcctctacaa agtcctttaa cactctcgta tctttgctgg caatgtttac
    21721 aatggtgctt acaagaccat ttttcttaac ccctgcatca gcagactggg gcagttgttc
    21781 atacaactca tacactgcct ccacagcatc atcaatatac ataaagttcc actgatgtct
    21841 gcatgcgctt aaagaaacct tgtttcctgc tggaagttcc cgcacaagag tggaaatgat
    21901 agaccaggga tggtctcctg ttccatatac actgaaaaag cgcagatgca cataatggag
    21961 tcccagttct tcacataaag gcactgcctt ctggtaaaat tcccattttg cctttccata
    22021 ctggttcacc ggatgacatt ctatatcttc ctgcataaag ccatctgtaa gaccatactc
    22081 cactctggaa cctgcatcca taaacatacg gcagtttaat gctttcgccg cccggataca
    22141 atccagggag ccagctacat ttttcgcctg aacctcaggt gagtcgatct cctgacggtt
    22201 cacaccgccc catgccaggt ggaaaaaggc gtccgcttct ttaatataag aaacgcagtc
    22261 cattacattg cttaaaccgc aggaaaccac atgaagtttt tcatctttcg gaagtacatc
    22321 caggtgcgtg gactccgggc gcaccagcac aaaaacctcc gcacctttct ttaaaaacag
    22381 ttttgccaga ttacttccaa taaatccggt agctcctgtt ataacgatcc tcatctgatt
    22441 tcctcctaga ccatttccag gtgattggag cgcatggcac ggatctctac ctgatcccca
    22501 aaacgctttc ttatgatccc tgcgatctca tctgtatagc cgggagctgt gatcacaatg
    22561 gcatctacag gatgggcttc aaaatactcc ggcggtacga tgggaaggtg ggaagcaggt
    22621 gcatattttc catgtttaaa cggcgccgaa tcgatgatat attctgcatt ttctcccaga
    22681 caggtagttg ctgccagtgt aaagccctga tggctggctc cccatatgca cacagtctta
    22741 tgccatgccc tcagatactt catatacgta ttcatctctt cccgcaggtt tacgtagcac
    22801 tccagcaggt tttctgtttc cacctgtgga cgtttgcgca cgatcaggga aagagtatcc
    22861 cggttgatca tagaagcttc tactacatca aatccgcttc tctccatcag ggaagtcagt
    22921 gtatcaaagg tataatacgc aatatggtca cggatcagtt cataataact gctgtgctcc
    22981 ataatgtact caaaacttgg cactgtaata agtcccatgg catcatcttc cagattattc
    23041 cagatagcac gcagcatagt caccggatcc ggctgatgtt ccaggaaatt aaaggataaa
    23101 aatacatcgt aaagtccacc tgggaacacc gtatcttctg tctctgtaaa gccctgtact
    23161 acatcaatgc ccttgctttt tgcaagttct accagagccg gatcatgttc tattccatga
    23221 acctctaccg gaaattcctt taaaacctgt aaaaactcgc cctggccaca gccaacttca
    23281 ataaaacgct ttccttccag atgataggtt tcgatgagat gtttgtactg ctttctgcga
    23341 agttctgcca tagtcgtaga atatccaccg gcacggatca catcccggta ataatccaca
    23401 ggttcacagt caaactgcac cagaccgcag ccgctgcact ggcaaagatc caaagtcagt
    23461 cccttatcag ctcctacatg ttctgcatct ggcatgtgct gcgcagacgc cggcatatta
    23521 tctaatgtaa gaaggggtgt ctcccagaga ggagccccgc aggcaataca tcgtttcatt
    23581 ttatatgttc tcctttttag cctcttttat ggtctctaag attccctctt taaaagaaac
    23641 ctgctggtga aaatcaatgg ctttttttaa ttttgtaaga tccggcatca aggaaacaca
    23701 gccttccgca ttcggcggcc ggtatccaaa ttcatagctt ccttttctgc cgcaaagctc
    23761 ataaacctct tcaatatagc tttttaacgg tcttgtatct tctcctgcca cattatacac
    23821 gcctgcagga actttgccaa gaagaagttt taccaggatc tgtgccgctt cctgcacata
    23881 cagaaagttc cactgctggg tacagggacc catttccata tgcccgttag ctaagaaagt
    23941 ccggatacag gtctcgatca aagaccatgg atgatcccct ggaccataca cgctgaaaat
    24001 acgggtatgg atgtagtcta ccccaagttc ttttgccgct ttggttgcct gctgatatac
    24061 caggactttg tcctttccat actcggatac aggatggcac tcctgttctt ctttcatcca
    24121 ttcattacag atcccgtatt ctgcctggga accggaaaac aaaaagcgtt tgcatccaag
    24181 agccgctgct gtgtgaagag cctgcagggc ataaccaatg ttcttttcct gaagctttac
    24241 atctttgcgc ttgtcacttc cagcgccgtc ccagcctaaa tgaagccatg cctttgcctg
    24301 caactcttct tttttgtaaa ttttttccag tttttcaatt tctcccagtt cacaaaaaca
    24361 aagttccagg ctgccgaaaa cctttttttc tgccccggcc tgctttttca ggtcctcaaa
    24421 aagttcctgc agctttcctg ccccggctga tgcagggcgt gatactgcca ccaccctgca
    24481 tccttcttct aaaagtgcct ttacaaccgc tcttccaata aaactggtag ctcctgtaac
    24541 gatcacggtc ataggattac tgatcctttc tatctttatg atgataccgc caacagacgg
    24601 aatatttatc ctttgatcct tggaatgatc atgttctcgt ccatttcctc atctggaagg
    24661 aatggtgcca tatcttccaa tggcggagaa accaaagttc catccggcaa ccgttttgcg
    24721 gaagacttcg gttcaaagtt ctggtctgta gatacaatga tctcacagat caccggtcct
    24781 tccatggcaa gggtcttttc tactgcctct gccagctgtc cgttatgttc tgctcttacg
    24841 taaggataac cataagcagc agacaatttt tccatgtccg ggaagcttaa gtcaccactg
    24901 tcataaccaa cacctactaa tggctcccca aagaaattct tctgggtctg acggatggaa
    24961 tgataaccgc cattgttaat aataaagatc ttaataccca ttttgtgatg gatgatagtc
    25021 tgcagctcct gaagattcat ctggatactt ccatctcccg taatcaggat cacatcttcc
    25081 ccttcacggg cagcgcccat gctgtacgcg ccgtcctttc tggaagcagc ccagataccg
    25141 atggctgccg gaaggtcata tcccatggaa gccactgctg agttggagat aaatctctgt
    25201 ccctttttaa tgatctgggc atgtccgcct actacgcagg cagagccatt tccaactact
    25261 gtgatctgtc cttcttttaa gcggctgctg atggcctgaa cagctgcata tacattagct
    25321 gccttgtctt cgccctgctc ccagtgtttt ttctgtacca ccggatattt tttcttccac
    25381 atagcgcagg tctcattcca tgtcatccct ttaagacctt cgccgtaagt aaatacctgt
    25441 ttctttccag gttcacttaa actgtccgga agtgtatctt tttctgcctc taatacctga
    25501 tccagacact gatccaatac tgacagcaga tctgctgcat ctgcatggac cggcatatct
    25561 acatgaaccg atggcttctt taattcctct tcatcaatat catttacgat cacataagcg
    25621 gctctggccc aggttttata gttatatcct acctgacgga tacttaaacg gcttccaatg
    25681 gaaagcacca ggtcactgtt ctggatagca aaatttcccg gacggtcacc catatttcca
    25741 ccgcggccta catataacgg atgttcatcc cagatacagt cctcactgtc ccagcctgta
    25801 acaaccggaa tacccagttt ttctgccact tttaagaaag tctcatgggc acctgccaga
    25861 cggatgccat ttcctgcatt gatcaccggg cgttttgcct gacggatctt ctcaatgatc
    25921 gcttttgcaa tctcttctgt taccttcttt ggaagaacct ggcgtttttc accttctccg
    25981 gcctcatctt ccgggatagc cggtacattt ttcttctctg cccagccatt tcctccgttt
    26041 tcataatcta ccgggtcaaa tccgatcagc tcatcggtct ccacatacgc accctggaca
    26101 tttaaaggaa tatccagcca gcttggacca cggcggccgg actttgccag atataatgct
    26161 ttttccaggc aatagcggat acgtaaagga tcaatgacca tttctgaata cttggtcata
    26221 cagtcaatgg ccttacaaat atcaaattcc tgatcaccca tagcacggat ccctgtaccg
    26281 gaccaccttg ctgtggtatc ataacgcacc tgtccggaaa gtaccagcat tggaatggag
    26341 tccagccatg cacctaaaac accggtaatg gcattggttc ctccaggacc tgtggtcacg
    26401 cagagagctg ccatctcatt atggatcctg gcatatgcct ctgctgcaat ggcgcaggcc
    26461 tgctcatggt gatcatagat acagtgaagg ccttcctggt gtcccaatgc atcatccaaa
    26521 tgcatggcac cgccgcctgt aaccataaat gcctggtgga tcccagcttt tactaacatc
    26581 tcagaaatat agtttgaaac tttcatcctc ataatacgtt ttctccttta agtcgtttac
    26641 gcatcaatct atctgaaaac aagcatacca caaaatgctt aaaaaaccta ctgtcatttt
    26701 ccttaagggg ctcctggata cgcccatttc caggtgtgga aaccatgccc agacttacca
    26761 gtaaccttca ctggaaaggc ggctttatcc tggttataat gacctgcgta atgcaaacaa
    26821 cggatcaacg gccactaaca aaacacattt tcaaaacatc aaaaggagag aatttatatt
    26881 atgagaaaac agacaacatt cctggctgta ttatccactg cagtatttac tgcatccatg
    26941 gcttttccgg catatgcaaa agcagccgga tggacggaag aaaacggaaa ctggtattac
    27001 tacgactcct acggagatcc ccttacagat acatggaaga aatccggaaa tgactggtat
    27061 tacttaaacg ctgacggcgt tcgcgcttat tccgaacaga tcgacgaata ctatgtaaac
    27121 gaagaaggaa aacgtgtcac ctaccagtgg gtcactatgg acaacgaaga ttactggtcc
    27181 gaagatgacg ctcctgaatt ccttcattac tattatggaa aagatggcaa agccttaact
    27241 tccacctggg cttccattga cggaaactgg tactacttca acgaagacag catcatggag
    27301 accggctcca tccaggtaga cggctataac tattatcttg gcgaagacgg aagcagaaag
    27361 accggctggg tccttttaga agaagaaaca gatgatccgg aagatttgaa tgcctggtat
    27421 tattttgact ccaacggacg ccggatcgaa aatgagatcg acaaaaagat cgacggttcc
    27481 tactatacct ttgaggaagg aaaaatgcag actggctggt acaagcttcc tgtaaccact
    27541 gagaacactg ctgcatccga caatactcag gcagaagact ctgcagaaac tgcaactgct
    27601 tcccaggcaa ctgccgcaga tcaggaagat aactctgtat cccttccggc gatctccggc
    27661 tataaatact atgatgaaga cggaaaacgt gcttccggct ggcgtaccat tgaaggcgtg
    27721 gaaaatatca gcgaagacgg tgaatatttc cgtttctact tcaagaacgg tgctccttac
    27781 tacggaaaag aggacctgga gatcttcacc attgactcca aaaaatatgc cttcaacaca
    27841 gcaggtgaaa tgcagaccgg caagcagtcc atcaggctct ctgacggtac agctgccaat
    27901 tactactttg acgaagaagg tattatgaaa accgggaaac agaccattta tgatgaggac
    27961 cttggggaga cccagaactg gctcttccac gcagaaggca gtaaaaaagg acagggctac
    28021 catggtatca aagataacac cctgtatgta aatggccttc gccaggaagc agacaaagat
    28081 ttacgttttg cccctgtttc cttagatgat acacgctatc tggtaaatgt aaacggtgct
    28141 gtacagaagg caggttccac ctccaagtct gcttccagac cagaacttgg cagcggatac
    28201 aaagatttta aagacgaaaa cgatatgatc tggactgtaa acacagaagg catcatccag
    28261 taagatcatc cccaaaaacg gcgctccttc cccatccgtt cacaaaaagg agcgcccttt
    28321 tttgtgcatt ttgccgaatt tctgattttt ttaaaaacag gcggtatatt ttggagattt
    28381 tcgcgttatt tataatgtaa agtatttttc atcaatcatt ctacataaaa gggggatttg
    28441 tgatgagaga actcgccgtt ttttactgtc cgaaatgcgg atactatgct tactatcaga
    28501 cttcgcgcca tccacagtgt cccagatgct gccatccgga tgtaatgcat atggcgcgta
    28561 tgtattacaa agaatttatg gatatgggat gtcgtgagcg ggatgactac ctggctcagg
    28621 agatcatgaa ggacaaccct tctttcttaa aacggatcaa tgctcctcac aagcgtttta
    28681 acagccgtga gattattgca gaaatgaata tcgagatcat gcgtctggac acagaaaaca
    28741 agatcttaag cgacactgtg aaatggatgc acgacaccat ctgggagatg atccgtgaac
    28801 agaagggcta taccgaccaa agtgccgccc agatcataaa cagtctgcag gatgacaaat
    28861 ccacccatac agaccattag aaaaaataca tgaaaacgat ttttataccg atgatcactg
    28921 atcatctaac tttaataagc ctcccggatc atctgtgatc atatcatcta cctgagcgct
    28981 ttcacacttg cgcagggagt acagatcatt gaccgtccag gctgtgacct tctttcccag
    29041 gctgtggatc tgatccaccc ggtctttccg gatcagctca atactgagca tatactgatc
    29101 tgcagccgga tattggctga aatcttctat atcctttgac cggatatatc cagtcttgag
    29161 ggatggttct acggctttta ccttttccaa aacagtatag cagaacgatg tgatcataca
    29221 ctggtctgtc attcccttat cccggatcag ctgtatgaca cgttctgtaa aatcactgct
    29281 ttccgttccg tcctctttta tttcaatatt cagttttatc ctgcctttgc aggtatccag
    29341 cgtttcctcc agagtcggga tctttacatt attatatcct ggtcccatac tggcggcagg
    29401 atgaagtttt ttgatctctt ccagagtcat ttcccggatt gttccatcct gtcccatgag
    29461 acgctttaaa ctgctgtcat gaaataccac cggaacatta tccctggtct cctttacatc
    29521 cagttcgatc cagtctgctc cgctttctat ggccttttca aaagcaggga tcgtattttc
    29581 cagtgctttt atagagtcac ctctgtgggc agtgatctct actttttggc tccatgctgc
    29641 atccaaagcc aaaacaggcg ccgcctgtcc caggcaaata caggcagcca tgagaaatac
    29701 aacgcctccc ctgactgcct ctttttttct tttttttatt ttcaaaatca taaatatcac
    29761 ttcctgaaac aaattttcac ttgaaatgtc tcctctggtg gcatatcata gtacataaag
    29821 ccacatttac aaaagaggag taaaaaatga gtatcaacga attgcttaac gatttaaaag
    29881 aaacctattc tttttcaagt gtaacctgct cacagaaacc agacgatgct atgactgact
    29941 tcgtattcta cctgactcag gaccagatca caaaagtcat cgaaaaagcc agccgcttaa
    30001 attccatcgt ggaatcctgt gcaaacatga tcagcatctg cgaaccggaa ttaaaagata
    30061 ctttaatggc aaccaccctt cgctgtgtag gagcaaacga acttcacatc cgcacctgcg
    30121 attccatgat caaaatgctg atccagtctt tattcgactg atagtatagc acgtattttt
    30181 ctaaaaatac gcatcttaaa aaagctttca taaaactgta aatatttttt tagacatccc
    30241 ctttcaaata aggtacaata taggtataac aattacacag atcagttata tcacgtatcg
    30301 gaaaggggat ctttttatgc agaaaaaaca ttttcgtatt cttgtcgccg cagcctgcct
    30361 gtgtgcagga ctatcatttt ccgcctatgg agaagaaacc atttcctctg tacctctctc
    30421 attttcctgg gatggggcac caaaaggtgg ggaggctgtt ggaacgctta ctgccaccac
    30481 taccagccag tcttttattg tagaaggcag tgaatatctg aaagacgatg acacctggga
    30541 ttatggggaa tggccctgcg ctgaaattga actttccgct gctgaaggtt atcgtttctc
    30601 cactccaaac cgacgtacct tctctctgtc cggctgcaat gtccagtata aaagctcaga
    30661 gattgacagc gacggaagca cactgatcct tcaggtttat ttatcccgca tcggcggcca
    30721 gctccctaag atcacagatc tgtcctggag cggaaaaaat gccgtatggg acaatatcga
    30781 aggatgtgac ggctatgaag tctgcctgaa aaaagacgga aaaacccttg taactgtcac
    30841 ctccaaagga acctcctatg atttttccga ctatatcaac aatgccggct cctatgtctt
    30901 tacggtccgc gccgtaagca cctacggcag ccagaacagc agctggtctg ccggttctga
    30961 gccttacatc ctttctgtag aggacgcatg gtacagcgac aatggaaact ggaaaaaaac
    31021 tggaaataaa tggcgttttg tatacaaaga tgagtcctac cctgcctgca gctggcggga
    31081 gatcaaagaa aaatggtatt actttgacgg aaacggctac atggtaacca actgctacgt
    31141 caaagcactt ggagaaaacc tgtactactg gatcagcgaa gatggtgtat gggatgaaaa
    31201 tgaaaccacc gcaacgccaa accgggcaag gtatctggtt tattgacctg ttgttatttt
    31261 gtaaaatcca ggtggatctg gtatctgatc cggtcaatat cttatacttt tgaaaaagca
    31321 gccgccaagg aatatatgtt ccttagtggc tacttttcct aattttcttg gatcacaata
    31381 tctttttcaa ttccagtcct gtacgtttca catattcccc aacatacttt ctgaaatcct
    31441 ggtatctaag cattttcagc ccataaagta ctgaccggta ccagtacagt ggcagtatct
    31501 gccatttttt catgtttaga tactgtctgc aaaaatagat ctcactgcaa atattgcagg
    31561 tataagcaaa tgcttttacc ttctctgtac tgtttccgtg aaggtgatgg atcacactgt
    31621 tgggatcata caccgttttc cagccttttt tctccatgga aattccaagt atcagttctt
    31681 cctcatataa aaatggtgtt tcatctaacg gagtcacatc cagtgcacat ttcctgctaa
    31741 acataaagca gcaccccata actgcatata cctgaaaaac ttccgactca taatcatgct
    31801 ctcttcccca ataactatta tttaacctgg ggaaaaacag ataaaaacgg gttctgagca
    31861 aatatttttc tctgattccc gtttttctca tcatacattc tttctgtata cttccatcac
    31921 gcagaaaaat cttcggtcct acaattccca catctgggtg atcctgtaaa tacgtttgca
    31981 gttctgatat acttcctgtt tcatagcgca catcagaatt gctgatcagt acttcttcac
    32041 agccatcctc aagtgccctt tttatcccaa tgttatttcc agccgaataa cctgcattcc
    32101 gttcattctg gataaaaata actccccttt tatccaggct ttccaacaca ccaggttcag
    32161 gacgcaccgg tgaacagtta tctaccagat aaatacgaaa ctccaatttc ttttctgctt
    32221 catgtatact gtctacacac gcaaccgtat ccgcccagct gttataattt aaaatgacaa
    32281 ttccaaccat atcatttcct tctgcgtcta tgtctccgtc tatactttct caggtctacg
    32341 ccattatctt ccagttttat ttccattaaa gtatcatgtg ggatcaaacc aacgatcagc
    32401 ggacggatca catatataaa tccccatgga aggagtccca atgctttaaa tccctgatag
    32461 cggactttca cctcatcaat acgatgcgca taggttgtct tatcaaaatc caccgtataa
    32521 cgcagcatag gtctttgaag attacagcca atatatccca ttgcataaag acgcataaaa
    32581 aggtcataat cttccattct gcgggtttcc ggagcgatcc tgtatccgcc ggctttcata
    32641 agcggacctt tcctaaacat cgttgccgga tgtataaact gagagccctt taaaaaatct
    32701 ttcttttcag gaaattcttt tctgtgccag taatcttcaa taatcccaaa ctctttcgta
    32761 agtccattta cctggcctga tacaaaggca taataagtat tcacatccag aaaatcattc
    32821 tgtgccatca gacgccacgg ataactgtaa tcatcatcat ccatacgagc tatataactt
    32881 ccagaagcat gctccagaca tttattcaaa gtagctgcca gtttcatatt cttctcatta
    32941 cggattggaa cgatcctgga atcgatcttg ctccactcaa ccagcttctc ccaggtatta
    33001 tctgtagacg catcatcaca tataataaac tcccagtcag taaaagtctg cacacgaata
    33061 gacgctattg cctcgtccac tctgtccgca ccattataaa cacccataat aacagatatc
    33121 ttaggccggt cccctactat acttcccatt tgtttacgtt cttcaccgta acgcggttct
    33181 gccatattat atacctcgta atactttcat tttatatatt tcattataag aaaggatttc
    33241 tctgccggca acttcttttc tgtgatttta tagagtattt tcatggatct caagaagatt
    33301 tcacagttac atgtttattc tttcaacaat aaccttacat ctctttttgc aaaatgttat
    33361 tccataaaat aaaatatttc gtctgtcatc attcagtaaa tactcataat atctttgatt
    33421 tttgatctga gcaatcgctt tctgacgtgc ctgttccatt ctctcaaatt cttttgtgta
    33481 tttcagttcc acaataatgc ctgaatccgg atcatctgtt tctgcaacaa tatcagcaaa
    33541 tccctcacct gcttcaatat ttgattttat caaccaatcc gcatttccgg taagtattcc
    33601 aaccagcaga ttatgatacg aactttcttt ctcctcattt ctggctttgg tatcaaatac
    33661 actgacggag ttacttaata ccctgttaag atatttttct attccttccg tatttccttc
    33721 ttcaaccgct ttccagaaga attgcagctc ctccgtattt gaaaattgcc actggtattg
    33781 atccaaaaag cttccggacg ggcaccaggg tcatccttga tcctgtcaac atagttgatc
    33841 acatcccatg gacaatatat atccacattt ccgaaacgat aaccatcata cca
//
LOCUS       DBMDUA010000008        33083 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1046_length_33083_cov_3.620975, whole genome shotgun sequence.
ACCESSION   DBMDUA010000008 DBMDUA010000000
VERSION     DBMDUA010000008.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 33083)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 33083)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33083
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1046_length_33083_cov_3.620975"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="ACZPCL_01375"
     CDS             <1..387
                     /locus_tag="ACZPCL_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR1161174.1"
                     /translation="GTGLELMGARQNMIIAARAAGVQCFDTVYTNLDDMEGFRHDVET
                     IHLMGFDGKSIINPRQINIVHEIFTPTQKDIIFAEKVVKEIDEKKKLGIGVFTVDGKM
                     IDIAFYDGAKRTIELAKASGVYKGDL"
     gene            389..1945
                     /gene="citF"
                     /locus_tag="ACZPCL_01380"
     CDS             389..1945
                     /gene="citF"
                     /locus_tag="ACZPCL_01380"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008755116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HIR1161175.1"
                     /translation="MINAVGRDIPEEILEMTGKEVFQGNHYRDGYVYKKDGPYTKCVV
                     NNTQSKLVANIHDVLIKCGIKDGMTLGFHHHFRDGDYIVNMVMEEVHKMGIKDITICA
                     SSLGKAHDPIVPYIEDGTITNIQSSGVRGKIGEAISAGKLKGLAIMRSHGGRVRAIES
                     GETRIDIAFIGTPTCDDYGNCRGIGGKSDCGVLSYAMVDGDYADKVVAITDCLVPFPN
                     FPAHISMTKVDYVVVVDAIGDPKKIATGAAKPTTDMRKLMMADYCTQFVVNSPYFKDG
                     FSYQTGVGGASIASTISLAKIMKERNIRMRFGVGGLTKPMCDLLINNQVDCLLDTQDF
                     DLAAVESVKNLKHFRISAGEYADPFNKGAVVNKLDFVILAALEVDVNFNCNVVVGSDG
                     VITGAQGGHPDTAAGAKCTIVIAPLLQGRIPAICSEVTTVTTPGESIDVVITDYGIAI
                     NPRRQDLIEAMKGVDLPFKTIEELRDIAYSIVGEPEKVQFGDRVVGIIEARDGTIMDV
                     VRQVKPYEFA"
     gene            complement(2096..3817)
                     /locus_tag="ACZPCL_01385"
     CDS             complement(2096..3817)
                     /locus_tag="ACZPCL_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1161176.1"
                     /translation="MKESVKKLEALMEIGGIKKDICFLVISGIALIISIFKLIPLPFD
                     AAWVAIILCGIPIIMEAVIGLITEFDIKADVLVSLALIASVFIDEDFAAGEVAFIMQL
                     GALLEELTVAKARAGIEKLVRLTPQTARLVKDGTETIVAATDVAVDDILRVLPGETIP
                     VDGVILSGNTSVNQAVMTGESLPVDKTVGDEVSSGTVNQFGTFEMKATKVGEDSSIQR
                     MIRLVQSADAGKAKIVGLADRFATWIVVIALSAAALTWLFTGQIIRAVTILVVFCPCA
                     LVLATPTAIMAAIGNATKHGFLVREGDALEKLAKVSKITFDKTGTLTCGTPKVVTACS
                     AHPGYSSEDIYRFCAGAEQHSEHPLGKAVLGCFKQKYGAVPAVYDFHMIPGRGVSALI
                     EGKMILAGNMEMMTENRISFNQDITEKAEGFLAQGCTVIYVAFDGILAGFLALSDTVR
                     MESTSMISRLHSLNVEPVLLTGDHENAAASIAMGGVGSDIAVDAADIALVDDEVKEIP
                     HLVALSKRMMTTIKVNMTFSMALNFLAITLAITGKLNPVVGALVHNAGSVLVITNSAL
                     LLKWKLK"
     gene            complement(4068..4343)
                     /locus_tag="ACZPCL_01390"
     CDS             complement(4068..4343)
                     /locus_tag="ACZPCL_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006597816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HIR1161177.1"
                     /translation="MRQCMDSDNLHRRLKKIIGQVQAIDRMIDEDIPCEEILSQINAA
                     KSALNGCGKVVLEGHIKHCVRDGIEHGDADKTIESFTKAVERFANMQ"
     gene            4564..5613
                     /gene="hydE"
                     /locus_tag="ACZPCL_01395"
     CDS             4564..5613
                     /gene="hydE"
                     /locus_tag="ACZPCL_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HIR1161178.1"
                     /translation="MKRSLELAEKLIHQSYLEKEEYMELLGSYQDKETVVCLGQEAAR
                     LREKYYGNKVYMRGLIEFTNFCKNNCYYCGIRRDNKNASRYRLTEEEILDCCKNGYEL
                     GFRTFVLQGGEDPYFTDEKIVDIVKKIRNSYPDCAITLSIGEKTKESYRLYKEAGADR
                     YLLRHETANEDHYRHLHPEQMSLANRKQCLRNLKELGYQTGAGFMVGAPGQTLECLAE
                     DLIFLKELNPEMVGIGPFIPHHDTKFADEPAGSVELTLFLLSVLRIILPKCLLPATTA
                     LGTADPKGREKGLLAGANVVMPNLSPVKDRKKYELYDNKICTSDEAAECRVCLKNRVK
                     SVGFELVEDRGDVAL"
     gene            5674..6213
                     /locus_tag="ACZPCL_01400"
     CDS             5674..6213
                     /locus_tag="ACZPCL_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR1161179.1"
                     /translation="MGHFYFVRHGQTVWNVEGKICGATDIALTQLGHEQAIETGKKIL
                     EEHIQADEILYSPLMRAADTAKHISEITGIPVREELRLKEQNFGIWESTPRGGEDFKK
                     AKQSFACSYETGESMLRLAQRIYNLLDEIKSQSDKKTYILVAHNGIARVVQSYFHDMT
                     NEEFAAYGIKNCEIKRYDF"
     gene            complement(6383..7429)
                     /locus_tag="ACZPCL_01405"
     CDS             complement(6383..7429)
                     /locus_tag="ACZPCL_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR1161180.1"
                     /translation="MSETTEFKDVYEAIASVSPNRQNILYTVLEEDAFGEKAFFSDDK
                     LVWESTKGGFFSQNQEEVGKLHDSGQATAAGKKLFCDCLGGEKRLVICGGGHVSMPVI
                     QLGAMMEFTVTVLEDRPLYANNARRAGAHQVICDSFENGLDQIEGSKDTYFVILTRGH
                     RYDRVCLERIAKMPHAYIGMIGSRKRVAKVKEVLIENGTDPEVLNQVYTPIGLNIGAE
                     TPEEIGVAIMAEIIQVKNQKLRSCGFPTEVSRFLNNHSPERLPAILTTIVARHGSAPR
                     SVGTKMLVLQDGTIIGTIGGGCMEAAVVRRALEMIHDKDQSPILYHGDLTGQDAEDDG
                     MVCGGVVDILMERV"
     gene            7896..8450
                     /locus_tag="ACZPCL_01410"
     CDS             7896..8450
                     /locus_tag="ACZPCL_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1161181.1"
                     /translation="MITDETIYQQYLRGETNAADLLVEKYADMLIFYINGYIKDVHEA
                     EDLMIEAFSQIFAKERPITGEGSFKAYLYKTARNLALRHSKKHRHIFLWIDDLDFELP
                     DKTLAETKVFRNERDRQLYAALDKLKPEYREAVYLIYLEEMSYRNAAAVMSKSEHQIK
                     NLVYRGKQSLKAILEQEGFEYADE"
     gene            8437..8787
                     /locus_tag="ACZPCL_01415"
     CDS             8437..8787
                     /locus_tag="ACZPCL_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HIR1161182.1"
                     /translation="MRTNEERSRLIHKRTAEIKREKQNRIQRGLDAVCIAVCLLLVTG
                     IGAWMPELMTEPAAGNIVHSSGAASMIGSHAALGYIVMGLLAFLLGVCVTVLLYRLRR
                     RNERRSREEKEDEL"
     gene            8777..9388
                     /locus_tag="ACZPCL_01420"
     CDS             8777..9388
                     /locus_tag="ACZPCL_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161183.1"
                     /translation="MNFELIDNSFQITILGFCTIAALFLALRYKSRSLLILALAYACF
                     CMGTTYYILYLVIMGKVPQVFYVAEISWLAAWLFYLSVQILRIERIGYRFSWFAGSAA
                     VLVAVVAFADHAFGPSYFFSALFALVAGVNMYLSVFGMQSVQPYRSTDGLMAGCIVLQ
                     VLLYLVSDFIQDYTCFNLYFAVDITLTLSMVALLPFTLREVGK"
     gene            9385..10026
                     /locus_tag="ACZPCL_01425"
     CDS             9385..10026
                     /locus_tag="ACZPCL_01425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005348701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR1161184.1"
                     /translation="MTYIENIFLCLAIPMILSLFFIKGRSRSYTLFVTIGMGICLLSA
                     YVNSFFMWLYGVDGTVAAIEITPVCEELLKLLPLLFYFLIFEPEPRELPMVAIAIAAG
                     FATFENACYLTENGAANFSFLLIRGISAGGLHILCGILSGFGISYVFRHRWLSVTGTA
                     GILGACTGFHGIYNLLITADGGWKTAGYLFPSVLILCLFIAKILLPKFTISLK"
     gene            10178..10489
                     /locus_tag="ACZPCL_01430"
     CDS             10178..10489
                     /locus_tag="ACZPCL_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161185.1"
                     /translation="MNGITEIRMRKIQSRTRQYRRRYEWREISCLTVCSLFLLAGIGI
                     LLGHIQEPGISSVAEGYGAVLLRNGADLYVTIGIASFTAGVVMTIICIRWRKKKWPYI
                     G"
     gene            10505..14710
                     /locus_tag="ACZPCL_01435"
     CDS             10505..14710
                     /locus_tag="ACZPCL_01435"
                     /inference="COORDINATES: protein motif:HMM:NF040368.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1161186.1"
                     /translation="MNMKKRLNRMLSTLLICSMVMTLMPTTVFATNRKDAVMEEYAGG
                     LCEHHPEHDDECGYSEGIAGTACSHEHTDDCYKEVINCTHRHDEDCYPDEIIWDDEAT
                     PSDADRWEPEECPHMCEEESECISQKLNCRHKHDDECGYSPATRGTPCEFDCEICGVD
                     DDMKTATPSNAKAVTVAGVQAMIDALPDVEDVRKGNAEKVKAQLDAIDDAKAELSDEK
                     IDELDLFRYMEVAAALEQLLYGVATQSNAVMLADYDGPAIQLGTGGISGPTEEEEEGK
                     GSYYVPNSYVYFGVNSGNSSTPIKWRVLDADTANDGTTSGMFLLSEYLLASDVQFNSN
                     YSKGNAYQGSDAQTWCSSFVGNTSNFSTAEQAVMFGVAKTDSGESGLYSIPWGESRLT
                     ANDKLFFLSVRELADYVGNYCDAPGLAATNTAQSAGVWWLRSPDSGIGYYTGTVYDDG
                     EVVNSLVNHDWAARPAFNLNSDSILFTSAADGGKTDAAVNGNLTEVGTGSAEWKLTLK
                     DTSRSFSASASSTLVRVGENLTVTYSGAGTGKNEYVSAMLADNSGNILYYGRIAQNSA
                     NGTASVEIPSDLTAGTYTLKVFSEQYNGDYKTDYASEFQNITLTVNGKVSEQFNLTPG
                     TTYWFDLSSAGIPGSTNNALPDTSRHYVPFTYVGSLNAYVLKPASENVLTSSEEASQV
                     TDKDAKYGYTYKHSLFIADYTMTHTISWKILNDNKLIFGTDFQNNRVAYTLRAPSAGH
                     TYAYGDNGGSTPDNNEWDTILKKANQSTQTNVSDYIKNWEGIWSYGQDTANTSAFRQF
                     RTIRGRMISAFSCNMEHKAASLPKYGFRPILEILDADTLGYDGLKTIVLDLNDGKIGD
                     TTGTVNIVVKNSESFTAPESSGLSSPIGKTDTDFCWQGSDGNSYIPGADVPAGVTSLT
                     AQWTPMTYTVMLETNGGTIADGKDVISYTYGEGATLPTANDMTREGYTFDGWYADSSF
                     SSAPVMEISNTDIGNKEFYAKWNANIYAVTLNPNGGTIASGKDITSYTYGNGATLPTS
                     NDMTREGYTFEGWYADSLFSGAPVTEINSTDMGNKEFYAKWTRNTTPIIPGDTVNYIV
                     EHYKAGNNGYMLEETEKLGDKIGANVTAEPKIYTGYTYNPDVAGSVTSGTLKKISSAA
                     DIVTLKLYYDLTVYNVTVENDGNGSASAAPASATMGGKITLTVTPNSGYRFKEWQIVS
                     GNVTISDDTFTMPAGNVTVKALFERKSSSGRDSSSDRDTSSSAIRKDPIRGWTSSDRG
                     IITGAANSIANDGYSHWMQDDHGWWLRFADNSYPKGQKRGTSGTAYVWELINGSWWAF
                     DENGYAKIGWLRDDTFGGWFYIDPERGMQTGWVRLGGAWYYFHQVSDGRKGIMYAGRK
                     TPDGYYVDENGAWMAKKNKSAGI"
     gene            15176..16675
                     /locus_tag="ACZPCL_01440"
     CDS             15176..16675
                     /locus_tag="ACZPCL_01440"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HIR1161187.1"
                     /translation="MALLNYDVLKKSGYDGYILEDAPVKVLQFGEGNFLRAFVDYWFD
                     LANEKAGWNGKCCLVQPIAPGLSKMINEQEGLYTLYLRGSENGKKVDDKRVISSVANC
                     LNPYEKADYDKMMEVAASDDLQIIVSNTTEAGIQYDPSCKKDDCPPASFPGKLTQVLY
                     HRFQAGKPGIIMLACELIDNNGKELLKCVNQYIDQWELGADFKKYVNEDCIFCGSLVD
                     RIVPGRIRDAEEVKALDAKNGYEDPLTDVGEVFGVWVIEGDEKLNDVLPFKKAGLLDK
                     VFVTPDMSPYKKRKVRILNGAHTGFVLGAYLAGENIVRDCMNDETIKGFMNKMLYDEV
                     IPTLPLDKNDLLNFAAAVSDRFNNPFVNHELMSISLNSTSKWKARNMPSFLEYIKEKG
                     ALPECLTMSLAAYIAFYSNDIQSLTDAGLVCKRPAGNEYTVSDDRWVLEFYNDHKDAS
                     AEELVHAVLTNEKMWDQDLTQIEGLEAKVAADLKLIREKGAKAAYASCL"
     gene            complement(16780..17718)
                     /locus_tag="ACZPCL_01445"
     CDS             complement(16780..17718)
                     /locus_tag="ACZPCL_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1161188.1"
                     /translation="MEETYYEMDLPRENIHLRCFAHSIMNYRYHWHPDEYELSIVLKG
                     NQEYCRGTETHSLFEDDLILTAPGNGHASMRQQPDTRALVLHFPSNALKLLVKKGYIY
                     QFPSCLSNENNRYEERFCRIRFYASQIWQSLECGGTYAQLSAKANLELLLITLFTEFD
                     PQQINLISEEDKRRATIHLLLSYVEEHYAEKLSLEDLADYAQYNRTYVSTLFKQMVGI
                     NFHEYLTRVRFQHALNDLTYTRDNLTDIALKNGFPDLKTLTARFRSTFQRTPAQYRAA
                     LKPDEVLYDKVRQFLDPQDMVLRKKLGEYAKTGIIK"
     gene            18165..19460
                     /locus_tag="ACZPCL_01450"
     CDS             18165..19460
                     /locus_tag="ACZPCL_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR1161189.1"
                     /translation="MNTQMMICMVIFAATLVSYMMNKIPMWLTAMLSLTALYFTGCID
                     ATGALAGFSNANTLLMATMFIVAAGFRRTSLVDGMCDAIMKLTRGSFLTAYFGYILIA
                     VLLTNFIASPMVVYAIVSPLLAALCDNTGHSRTQYMFPVMVVCVACCGILPLSTAIQQ
                     AGQFTGFMETYGFTDMSIRAVDFTIGTWPILIIVPLWALFIGPKFTPKTPVVPIEALE
                     KRNAGKQEQKLSPVVDKIGIAIFFGTILCLIFSSQLHLATWSVALTGSLLMVLFGVVD
                     QKAALRDIPWDMLMLFVGALALGTALTNTGAGDMIGNTLASAVGGTHNNYVLGALFFL
                     IPFVLTQFMLNRSVSAVFVPICLLTSSALGANPVGLVLLVNAGSLTAFLTPMATPAVP
                     MCMADGGYDLRAIFKSGALITLILPFIYIFYTMTVFPAF"
     gene            19479..20381
                     /locus_tag="ACZPCL_01455"
     CDS             19479..20381
                     /locus_tag="ACZPCL_01455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1161190.1"
                     /translation="MAMTQEEKIEIADMVRSRDRFGPVSEEELAALPGKETELWIRTK
                     NGKTVHVFEERPENLPEQAALLLNFHGGGFLKGRTDRDRRYCCWAMEQLNCLVWDIDY
                     SLAPENPFPFAAEEAYEIVSYAFEHSTELGIDQEKIILAGHSAGGNLVASVLIKNEET
                     KELHPCCALMEYFPTDNTVDPVERLSEELKKDPFWVKRAETEKMYTDFYVGDADPSDP
                     LCSPAFAKDQALAAFPDCLIISAGEDSLRDDTEAFGMRLAKAGVCVTMQRITEAMHGF
                     TTNRTPGWERALDRQYKFFQEHLR"
     gene            20424..21569
                     /locus_tag="ACZPCL_01460"
     CDS             20424..21569
                     /locus_tag="ACZPCL_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR1161191.1"
                     /translation="MKITDVKLRFAKHYLFVQIYTDAGITGLGEAGNWGYLQATAAAI
                     EKFADYLIGKDPFRIEDFNQNFLRSVYFRGSVIMSAISAIDIALWDIKGKALGVPVYE
                     LLGGKTREKVRVYASVMHLTEDNNELAKQYQQLQEMGFTAAKIFCNGPVSAPDGKGEF
                     YSSRIEREVEKVRVCREAVGNDFDFVLEVHRGMTLPEAVAFGRAVEPYRPMILEDPVP
                     PDNVDTMAEVASKVGVPIATGERFIDLREFEVLMARHACQYVRPDVCAVGGITTSKKI
                     CALAEAHDVLVIPHNPLGPVSTAACLQICASIPNLGIQELPGFCLNGAEDKMVKEPLR
                     FENGCMLIPEAPGIGVELADDAEGLYPANERGSNAARRAFDGSVKDW"
     gene            complement(21810..22592)
                     /locus_tag="ACZPCL_01465"
     CDS             complement(21810..22592)
                     /locus_tag="ACZPCL_01465"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR1161192.1"
                     /translation="MYRRTKCHFVHYITQYKKKSRQKAAASIRTLQHGTKSGQLFWKT
                     GAVFASSDCIEIHIHGKGGHASTPFLAINPILIAAEIINSLQGLVSRESTYNVPAVLT
                     VTGITAGNGAYNIIPNSATIVGGLRTQSLEVRSHLLKRMEEISKQCAAMYGATAEMKV
                     PSGCPPVINDPTMTADFITAAKKVFPAEDILELDHSSMGGEDSSYFFTEVPGCYAFLY
                     NSIPSDDGKEYPHHNGRFCIDDSVLYLASAVFAQAVADQICK"
     gene            22643..23959
                     /locus_tag="ACZPCL_01470"
     CDS             22643..23959
                     /locus_tag="ACZPCL_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1161193.1"
                     /translation="MYHDLTKGNISRSLLLFALPMMAGNLLQQFYNIADTLIVGRVLG
                     RDALAAVGSAYTLMTFLTSIFLGLSLGSGALFSIYKGRGDEKTLKSSVFHAFVLIMAV
                     TVVLNILVYLGLDGILAFLKVPEEVWQGMRQYLLIIFAGLIATSLYNFFACLLRAMGN
                     STVPLVFLAVSTVINIVLDLLFVAVWSMGIGGAALATIIAQYISGLGITVFALRCCPQ
                     VIPKKSELKWNGGILKEIVNLSSMTCIQQSVMNFGILMVQGLVNSFGPVVMAAFAAAV
                     KIDSFAYLPVQDFGNAYSTFIAQNYGAGNRERIKKGTKQAFALSILFCIFISALVFIF
                     AGQLMGIFVSKAETEVIACGIKYLRVEGAFYWGIGCLFLFYGYYRAVKRAGMSVVLTV
                     ISLGMRVLLAYVLSAKVGEVGIWAAIPIGWVLADITGLVCMLRSKL"
     gene            24014..25624
                     /locus_tag="ACZPCL_01475"
     CDS             24014..25624
                     /locus_tag="ACZPCL_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161194.1"
                     /translation="MDKNEDRTIKNKVREKIQGVSMLPFGLIAGFAGEKEIRIIELAE
                     NGFTFRTAEKIADPELFRVCFYHYPKLEYEQVELHSYTLECSGEYEFYYEYTLYLDHI
                     KNKDIMWDSHFIDDNDSAEFIEYRKQVQYLLGWYDRYIRMKLLGDSEELASFMTAYPE
                     GGDVVTARDFEEQKKAWFFRETKEIHRDEKEVEAEKAKKTVQDAKQKLKTGLEVGSES
                     PVSEYALELDNPWLYEEYLSKDLTTFMENYWKRNYLSGHWLSDHFPQRLYIGNSFCHL
                     LFPKEDQLFQLLEKARKESLQVTLTFSYIREFMLENTGQLMEKLENWCEENKVTLEIQ
                     VNDWAMAEMLKGKVLFVPVLGILLNKRRKDPRLHYKTETMAQIGAETESKFLEENSLN
                     ADFYRKYLEKEYGIVRYEWESCGYDMALPPGKNSLHLPFYQTNTSQYCTLYAKCMTGE
                     RGKQKLAEECPKFCKDYAFLYPDHLMMIGRYNSLFALDSRIFEDGSGSGTGTEIGTKI
                     KTEEILKKWKKAGVDRLVVNLLDPGVKR"
     gene            25666..26766
                     /locus_tag="ACZPCL_01480"
     CDS             25666..26766
                     /locus_tag="ACZPCL_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HIR1161195.1"
                     /translation="MKIVAGLGSIDEYIPYVKAGADEFFCGYVPYSWTEKYGTLLPLN
                     RREVLCSNVQLGAFSELEILASMVKKYEKPVHLTFNSLYYIPEQYLEIANIILQCMSI
                     GFQSFIIADPALLLYLRQQRIDCDIHLSGETAEVNSEMLKTFEKRNLKRLIFHRKNSI
                     EDMGSIISKAGQTTEFEAFALNEMCQFTGAFCNSLHCDEMCHLCLVPYELGRIRECVS
                     AENRLKTSPLPVKLQENEMKNIRSAENVDEPEDDSYLCGQTGCGLCALYRLGKAGVTH
                     LKLVGRGNYTDYMERDIRNLRKALEILKDVLDMEKTGNIPAGLKAEETYISQMKKELF
                     GSAGKCSGMCYYMNVLSFCKNNRSGEHIKRRK"
     gene            26769..27812
                     /locus_tag="ACZPCL_01485"
     CDS             26769..27812
                     /locus_tag="ACZPCL_01485"
                     /inference="COORDINATES: protein motif:HMM:NF021778.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HIR1161196.1"
                     /translation="MRYAYVGCQTTKERNARGEGLKTYEIQPDGSWKCLQCLETEPNP
                     SFQTLDGEGKYLYSVHGDITKVSSYRILKDHTLEHINTIDIGGRNPVDITVDRNNKHV
                     IVATLQGGTIYTIDRKNDGSLGEVIAEFTYEGKEPGKVSTIHQCLWDKKKNFLFACAQ
                     SRVNGYGQMRALRYDHETGQFTQTAQFLSRTWDEPRHAAVHPNNLWVYMCEEKGNKVL
                     FFRFDDENGTFEALQELTTVPETVTGYSDASEVMVDPSGKFVLVSNRYTDSIAVYRID
                     PLTGYMRNVGFYPCLGKTPRFFCFGDNGKCYVANEDSDTIIEFDFDNMTGALIPTLNI
                     VHTGSPVCILFAD"
     gene            complement(27880..28440)
                     /locus_tag="ACZPCL_01490"
     CDS             complement(27880..28440)
                     /locus_tag="ACZPCL_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013282056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR1161197.1"
                     /translation="MRKNFGAKPYTYPQPVFMIATYDENGTPDVMNAAWGGISEGNEL
                     SMCISEGHKTTKNLLARKAFTVSMADAPHMTACDYVGIVSANKVPDKFEKAGFHAVKS
                     EFVDAPLIEELAVAVECRLKSYDAKTCRLVGEIVNVSVDEKVLDENGNVDIAKAQPIT
                     FDPFNNKYVVLGDVVGNAFTDGKKLM"
     gene            complement(28497..28871)
                     /locus_tag="ACZPCL_01495"
     CDS             complement(28497..28871)
                     /locus_tag="ACZPCL_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1161198.1"
                     /translation="MTIKEVSQKYNISSDTLRYYERVGMIPPVTRTAGGIRDYSESDL
                     GWVELALCMRGAGLPVEAMIEYVKLCQQGDSTIEARMLLLKEQMETLLEQKAQIESTI
                     KRLDFKISRYEEAVKTGKLTWE"
     gene            complement(28999..29856)
                     /locus_tag="ACZPCL_01500"
     CDS             complement(28999..29856)
                     /locus_tag="ACZPCL_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1161199.1"
                     /translation="MNHSNNHITELIKETLILTAAMAIVAGAVYFFLLPSHASVSSIS
                     GLGIILSNFVPLPLSAITMILNVLLLIIGFFTCGKEFGAKTVYTSIVLPLFLALFERI
                     FPNIGSLTDSQELDVLCYILTVSIGLSILFNRNASSGGLDIVAKIMNKYLHMDLGKAM
                     SLSGMCVALSAALVYDKKTVVLSVLGTYFNGIILDHFIFNHNVKRRVCIITKKEEELR
                     HFIINDLHSGATIYESIGAYNMEKRNEIITIVDKSEYQKLMNYINREDPHAFITVYTV
                     SDIRYQPKK"
     gene            30025..30516
                     /locus_tag="ACZPCL_01505"
     CDS             30025..30516
                     /locus_tag="ACZPCL_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR1161200.1"
                     /translation="MNIQVRAYTENDLPDMIRIWNEVVEDGVAFPQEEFLTEETGKSF
                     FAEQTYTAVAEDAETGNVYGLYILHPNNIGRCGHICNASYAVSRDSRGIHIGEKLVSD
                     CLKQGKEHGFGVLQFNAVVANNIHARHLYERLGFIQLGVIPKGFRMKDGHFEDICPYY
                     HEL"
     gene            30764..32992
                     /locus_tag="ACZPCL_01510"
     CDS             30764..32992
                     /locus_tag="ACZPCL_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HIR1161201.1"
                     /translation="MVETSKRLKGAIDMHIHSAQMYGREKTMTWKILKAHNHSNTMED
                     LQLVFDALVSPDNNYVSILQTARASGLKKFPVPYVLSNCHNSLCAVGGTINEDDHVFG
                     LGNAVKYGGIFVPPYKAVIHQYMREMMAGCGKMILGSDSHTRYGVLGTMGIGEGGGEI
                     AKQLLKKTYDIPYPKVIAVKLTGAPKPGVGPQDVALALIAATFKNGFNKNKVLEFIGD
                     GIDNLSVEYRMGIDVMTTESAALSSIWCTDEKVHEYLKLHGREEEYKEMMPEAGACYD
                     SLIEIDLSQVECMIALPYHPSNAIPISYFKEHMEELLKEVEEEGRKIKGDNGKPFSIM
                     SHIHDGGFYVDQALVSGCSGGMFENISAMADILDGYSISGDQCSLGVNPSSMPIMAEM
                     MDQGIAKQLMLSGATIRPAICGPCFGVTDVPADNQVSIRHTTRNYPNREGSKPGKGQM
                     AAAFLMDARSIAATVRNGGRLTAATELEVEYTDRKAGFDRSIYEKQVYNNYGKEKRST
                     ELKMGPNIADWPEMFPLKKHLLLKTVGVYEGSLTTDELVPSGDASSYRSNPEKLAEFT
                     LCSRDPEYVKRAMAVRETARQQEAGQKLSDQEMDGLLEQIAAKYGADPKEISIGSLLA
                     GVKIGDGSSREQAASSQKVLGGWADLAEEYSTKRYRSNLINWGILPLITKEPLVVKNG
                     DVILIQDIETVLKDGREELTAYLLDEKTGETKKEIPCSLGRLTAEERKILLSGSLINH
                     YK"
BASE COUNT         9508 a         6703 c         8153 g         8719 t
ORIGIN      
        1 ggtaccggtc ttgaactgat gggtgcacgc cagaacatga tcatcgcagc ccgtgcagct
       61 ggtgtacagt gttttgatac tgtatacaca aacctggatg atatggaagg tttccgtcac
      121 gacgtagaga ccatccattt aatgggattt gatggaaaat ccattatcaa cccacgtcag
      181 atcaacattg tacatgagat cttcacacca acccagaagg atatcatttt tgctgagaaa
      241 gtagttaagg agatcgacga aaagaagaaa ctgggtatcg gcgtattcac tgtagacggc
      301 aagatgatcg acattgcatt ctatgacggc gcaaagagaa ccatcgaact tgccaaggca
      361 tcaggcgtat ataaggggga tctgtaaaat gataaatgca gttggaagag atataccaga
      421 agagatcctg gagatgaccg gtaaagaggt attccaggga aatcattaca gggacggata
      481 tgtatacaaa aaggacggcc cgtatacaaa atgtgtggta aacaatacac agagcaagct
      541 ggttgccaac atccatgatg tgctgataaa atgcggcatc aaggacggca tgactttagg
      601 cttccatcat catttccgtg atggtgacta tatcgtaaat atggtcatgg aagaagttca
      661 taagatgggt atcaaggata tcaccatctg cgcaagttct cttggtaagg cacatgaccc
      721 gatcgttcct tatatcgaag acggaaccat taccaatatc cagtcctccg gtgtccgcgg
      781 taagatcggt gaagcaatct ctgcaggtaa attaaaggga cttgcaatca tgcgttccca
      841 cggtggccgt gtaagagcga tcgagagcgg tgagacaaga attgatattg catttatcgg
      901 aacaccaacc tgtgatgact atggcaactg ccgtggtatc ggtggtaaga gtgactgtgg
      961 tgtgctttcc tatgctatgg tggatggtga ttatgcagat aaggtagtag ctattaccga
     1021 ctgcctggta ccattcccga acttcccggc acatatttcc atgaccaagg ttgactatgt
     1081 ggtagtagta gacgctatcg gtgatccgaa gaagatcgct accggcgcag caaagccgac
     1141 tacggatatg agaaagctga tgatggctga ctactgtacc cagttcgtag taaacagccc
     1201 atatttcaag gacggattct cttaccagac cggtgtaggc ggtgcgtcta tcgcatccac
     1261 catttccctg gctaagatca tgaaggaaag aaatatccgc atgcgttttg gcgtaggcgg
     1321 cctgacaaag ccaatgtgcg acctgctgat caacaaccag gtagactgcc tgcttgatac
     1381 tcaggacttt gaccttgcag ctgtagagtc tgtaaagaac ttaaagcatt tccgtatttc
     1441 tgccggtgag tacgcggacc catttaacaa gggtgctgtt gtaaataagc tggatttcgt
     1501 aatcctggca gcactggaag tagacgtaaa ctttaactgt aacgtagttg taggttctga
     1561 cggtgtgatc accggtgcac agggcggaca tcctgataca gcagcaggag ctaagtgtac
     1621 tatcgtaatc gctccactgc ttcagggaag gatcccggct atctgcagcg aagttaccac
     1681 agtaaccact cctggagaga gcattgacgt agttatcacg gattacggta ttgccatcaa
     1741 cccaagaaga caggatctga tcgaggcaat gaagggtgta gacctgccat ttaagaccat
     1801 tgaagaactg cgtgacatcg catactctat cgtaggcgaa ccggagaagg ttcagtttgg
     1861 agacagagta gtaggtatca tcgaagcccg cgatggcacg atcatggacg ttgtacgcca
     1921 ggtcaagcca tatgagtttg cttagtcagc aacgaacaaa ccgcctgcgt aagcaggcag
     1981 tgaagcgcgt aagcgcgggt ttgtgagtct gagaagtttg tgagtctgca aaatttataa
     2041 gtctgaaaaa aaagtccatg ccgctgccgc actggcagcc tgcatggact tttttctact
     2101 tcaatttcca cttcaaaagc aatgctgaat tggtaataac caatacagat cctgcattat
     2161 gcaccagcgc tcccacaacc ggatttaact ttcctgtaat agccagtgtg atagccagga
     2221 aattcagtgc catggaaaaa gtcatattga ccttaatggt agtcatcatg cgcttggaaa
     2281 gagctaccag atgagggatt tcttttacct cgtcatccac cagcgcaata tctgctgcat
     2341 ctaccgcaat atcacttccc acaccgccca tggcaataga agcagctgca ttttcatggt
     2401 ctcctgtaag aaggacaggt tctacattca gactgtgaag ccggctgatc atagaggtac
     2461 tttccatacg cactgtatca gaaagagcca ggaaaccagc cagaatgccg tcaaaagcca
     2521 catagatcac agtacagccc tgggccagga agccttctgc tttttctgta atatcctgat
     2581 tgaaagagat cctattttca gtcatcattt ccatgtttcc agccagtatc attttgcctt
     2641 ctattaaagc agaaacacca cggccgggga tcatatgaaa atcatacact gcagggacgg
     2701 cgccgtattt ctgtttaaaa cagccaagaa ccgcttttcc aagaggatgt tccgagtgct
     2761 gttctgctcc ggcacagaag cggtaaatat cttcactgga gtagcctgga tgagcgctgc
     2821 aggcggtaac tacctttgga gtgccgcagg ttaaagtacc tgttttatca aaggtgatct
     2881 tagaaacttt tgccagtttt tccagagcat ccccttcacg gaccaaaaat ccgtgctttg
     2941 tggcatttcc aatggcagcc ataatggcag taggagtggc caggaccaga gcgcaggggc
     3001 agaaaacaac cagaatagta acggctcgga tgatctgacc ggtaaacagc caggtaagag
     3061 cagcagcgct taatgcaatt accacgatcc aggttgcaaa acggtcagcc agaccaacga
     3121 tctttgcctt tcctgcatct gctgactgca ccagacggat catgcgctgg atagagctgt
     3181 cttcgccaac cttagtagcc ttcatttcaa aggtaccaaa ctggttcact gtaccggaag
     3241 aaacttcatc tccaacagtc ttgtctacag gaagagactc accggtcata acagcctggt
     3301 ttaccgatgt atttcctgaa aggatcacac cgtctacagg aatggtctca ccgggaagga
     3361 cgcggagaat atcgtctaca gctacatccg ttgcagcaac tatggtttct gtgccatcct
     3421 tgaccagcct tgcagtctgt ggtgtcaggc gcaccagctt ttcaattccg gcccgggctt
     3481 ttgcaacagt cagttcctct aacaaagcac ctaactgcat gataaatgcc acttcaccgg
     3541 cagcaaaatc ttcatcgata aagacagagg cgatcagcgc cagagataca agaacatcgg
     3601 ctttgatgtc aaattctgtg atcagaccaa tgacagcttc catgataatg ggaataccac
     3661 acagaataat ggcaacccag gctgcgtcaa atggcagcgg gatcagttta aaaatactga
     3721 tgatcagtgc aataccggaa atgaccagga aacagatgtc tttctttata ccgccgatct
     3781 ccataagagc ttccagcttt ttaacagact ctttcataat ggatccttct ttctgcatta
     3841 ttgcaaatgt tatgtgagaa tgcatatgac ttttatacct atgggggtat acctaacata
     3901 tctgcattat acctataggg gtatatgttg tcaagaagca attataaaag gatccaaaga
     3961 gtaaggctct tttttgcaca caaaaaagcc agccctgaat ggactgactt tggatattag
     4021 tatatgatgt tagattgcgt tgaattttca atctttgaac actgacgtca ttgcatgttg
     4081 gcaaagcgct ccactgcctt tgtaaagctt tcaatggtct tatctgcgtc accatgttcg
     4141 atgccgtccc ggacacagtg tttaatgtgg ccttccaata ccactttacc gcagccgttt
     4201 aaagcggatt ttgcagcatt gatctgggaa aggatctctt cgcagggaat atcttcatcg
     4261 atcatacggt caatagcctg gacctgtccg atgatctttt ttaaacgtct gtgcagatta
     4321 tctgaatcca tgcactgtct catagtattt tacctcctta tccgcggatg aacctttgga
     4381 gtataagtaa gtgtaaccaa attttggcgg tttgtcaatg aagggaagaa ttgacaggaa
     4441 gtgttgtttc agagtaaact tgcaggagat gcattttact atgaaggctg gcggcagctg
     4501 tgagaacgct gactgtaagg agcagccagc tttcaggttt tgaaagaacg aggaagacag
     4561 aatatgaaac gatcactgga actagcagaa aagctgatcc accagagtta tctggaaaaa
     4621 gaagaatata tggaattatt aggcagttat caggataagg aaactgtggt ctgtctgggg
     4681 caggaggcag cgagacttcg tgaaaaatat tatggaaata aagtatacat gcgtggtctt
     4741 atcgaattta ccaatttctg taaaaacaac tgctattact gcggtatccg ccgggataat
     4801 aaaaacgcca gccgttatcg tcttacagaa gaagagattt tagactgctg caaaaatgga
     4861 tatgaactgg gctttcgtac ctttgtactc cagggaggag aagatcctta ttttacagat
     4921 gaaaagattg tggatatcgt taagaagatc cgtaacagtt atccagactg tgccatcact
     4981 ctttccattg gagaaaaaac caaggaaagc taccgccttt acaaagaagc aggagcagac
     5041 cggtatctgc tgcgtcatga aactgcaaat gaagaccatt accgccatct acatccggaa
     5101 cagatgagtc ttgcgaacag gaagcagtgt ctgcggaatt taaaagaatt gggctatcag
     5161 accggtgccg gttttatggt aggtgcgccg ggacagacgc tggagtgcct ggcagaggac
     5221 ctgatctttt taaaagaatt aaatccggaa atggtgggga tcggcccatt tatcccccat
     5281 catgatacca aatttgctga tgagcctgca ggaagcgtgg aattgacgct gtttttatta
     5341 tcggttcttc gtatcatcct tccaaaatgt ctgcttccgg caacaacagc tcttggaact
     5401 gcagatccaa agggaagaga aaagggactg ctggcaggtg caaacgtagt aatgcctaat
     5461 ttatcccctg taaaggaccg caagaaatat gaactgtatg acaataaaat ctgtaccagt
     5521 gatgaagcgg ctgagtgcag agtctgtctt aagaaccggg tgaagtcagt tggatttgaa
     5581 ctggtggaag acaggggaga tgtggcgtta tagtgtttac gccgcaaaag gtggaaaagt
     5641 atgttatgaa tttagtagga ggaaattaat ataatgggac atttttattt tgtaagacat
     5701 ggacagactg tatggaatgt ggaaggaaag atctgtgggg caacggatat tgcactgaca
     5761 cagcttggtc atgaacaggc aatagaaaca ggaaagaaga tattagaaga gcatatccag
     5821 gcagacgaga ttctgtattc ccctttgatg cgggctgcag atacagcaaa acacatttct
     5881 gagatcacag gcattccggt ccgtgaagaa ttacggttaa aggagcagaa ttttggtata
     5941 tgggagtcaa caccaagagg tggtgaagac tttaagaagg ccaaacagtc ctttgcgtgc
     6001 agctatgaaa ccggtgagtc tatgcttcgt ctggcacaga gaatttataa tcttttagat
     6061 gagatcaaga gtcagtcaga taagaaaaca tatatcctgg tagctcacaa tggcattgcc
     6121 cgagtggtgc agtcctattt ccatgatatg acaaatgaag aatttgcagc ttatgggatc
     6181 aaaaactgtg agataaaaag atatgatttt taaatcagga aacgcgtcac gggcgcattc
     6241 tccatatttt caatatgtat ggcaataaaa gtcccctcat cagcggttta cagagtaata
     6301 aacagctgat gaggggattt gtcataaatg actgtgtccg gtgtggaaat gcgtcagtta
     6361 tcttgaacgt ggtatatcag gatcacaccc gctccatcaa aatatcaacc actccgccgc
     6421 agaccatacc atcatcctct gcgtcctgtc ctgtaagatc cccgtgataa aggataggag
     6481 actgatcctt atcatggatc atttccaacg cgcggcgcac aaccgctgct tccatacagc
     6541 cgccgccaat ggtgccgata atggttccgt cctgaaggac taacattttg gtgccaacgc
     6601 ttcgcggagc agagccgtgg cgggctacga tggtagtcag gatagccgga agccgctcag
     6661 gagagtgatt atttaaaaag cgggatactt ctgttgggaa gccgcagctg cgtagcttct
     6721 ggttctttac ctgaatgatc tctgccataa tggcaacgcc gatctcttct ggtgtttccg
     6781 caccgatatt taagccgata ggcgtataga cctggtttaa aacttccgga tcagtgccgt
     6841 tttcaatcaa aacttccttt acttttgcca cacgtttgcg gctgccgatc ataccaatat
     6901 aggcgtgagg cattttggca atccgttcca gacagacccg atcatatctg tggcctctgg
     6961 taaggatgac aaaataggta tctttggaac cttcgatctg gtcaagacca ttttcaaagg
     7021 agtcgcagat cacctggtgg gcaccggctc ttctggcatt gttggcatac agaggacggt
     7081 cctctaaaac agtcactgta aattccatca tggcgccaag ctggatcacc ggcatggaaa
     7141 catgaccgcc tccgcagatc accaggcgct tttcaccacc taagcagtcg cagaacagtt
     7201 tctttccggc ggctgtagcc tgtccgctgt catggagttt tccaacttct tcctggttct
     7261 gggagaagaa cccacctttt gtgctttccc agaccagttt atcatctgaa aagaacgctt
     7321 tttccccgaa ggcgtcttct tctaaaactg tatagagaat gttctgcctg ttgggactga
     7381 cacttgctat agcttcgtat acatctttaa attctgttgt ttctgacatt ttaatccccc
     7441 gttttttaat gtactcttgg agtacatatc gttttgtcta tctgcagtat gctgatggaa
     7501 tatctgcaca gatattggaa ctcgttatta tatattttta ataacgcctg ctaaatatta
     7561 ttcattatag catcttccgg gaacaggggc aagcaacatt ccagtttctt cacttgaaaa
     7621 tgtgctgaca ttcgcatata ctaggtgcaa cagatttcag ataggatatt tttcataagg
     7681 ggacattgtc cttgctcatg tttataaagc aggtatgatg aacttgatca tatctttcag
     7741 aaaggcgatg tacgaatgaa tggctatgta tctgtaaaag aagcttcaca caagtgggaa
     7801 attccagaac atgggaccaa ttatgggaaa tcccgggaaa atcagaaaaa acgtttgatc
     7861 cgtgccagta gaaatcggat gggaggcaga caggtgtgat taccgatgaa actatatatc
     7921 agcaatatct gcgtggagaa accaatgcgg ctgatctgct ggtggaaaaa tacgctgata
     7981 tgctgatctt ttacataaac ggttatatca aggatgttca tgaggctgag gacctgatga
     8041 tagaagcctt ttcccagata tttgcaaaag agcgtcctat tacgggagag ggcagcttca
     8101 aggcatatct ttacaaaaca gcccgcaacc ttgccctgcg gcatagcaaa aaacaccgcc
     8161 acatcttcct gtggattgat gacctggatt ttgaactgcc agataaaacc ctggctgaga
     8221 ccaaagtctt tcggaatgag cgggatcggc agctctatgc ggcactggat aaactgaaac
     8281 cggaataccg ggaagcagta tacctgattt atctggagga aatgagctac cggaacgcgg
     8341 cagcggttat gtctaaaagt gagcatcaaa tcaaaaatct tgtgtaccgc ggcaagcaga
     8401 gtttaaaggc aattttggag caggagggat ttgaatatgc ggacgaatga agaacgttcc
     8461 aggttgatcc acaaacggac agcagaaata aaacgggaaa agcaaaacag aatacagcgg
     8521 gggcttgatg cggtctgcat agcagtttgt ctgcttttag ttacaggtat tggggcatgg
     8581 atgccggaac tgatgacaga acctgcggca ggaaacattg tccattcttc gggagcggca
     8641 agtatgatcg gcagtcatgc tgctcttggc tatattgtca tgggcctgct tgcttttctc
     8701 cttggtgtat gcgtaaccgt gctgctttac cgtctgcggc gcagaaatga gcgcaggagc
     8761 agagaggaaa aagaagatga actttgaact gattgacaat agttttcaga taaccatatt
     8821 gggattttgc accattgcag ccctttttct ggctttgcgg tacaaaagca ggagtttgct
     8881 gatcctcgct ttggcatatg cctgtttctg catgggaacg acatactata tcctgtatct
     8941 ggtcattatg ggaaaagtgc cgcaggtttt ctatgtagct gagatttcct ggctggccgc
     9001 atggcttttc tatctgtccg tgcagatcct gcggatagag aggatcggat accgcttctc
     9061 gtggtttgca ggcagcgcgg cggtgcttgt cgcagtggtc gcttttgccg accatgcctt
     9121 tgggccttcc tatttctttt ccgcgctgtt tgcactcgta gcgggagtga acatgtatct
     9181 ttcggtgttt ggcatgcaaa gtgttcagcc atatcgaagc acggatggtc tgatggctgg
     9241 ctgcatcgtt ctgcaggtac ttctgtatct ggtttccgat tttatacagg attatacctg
     9301 ctttaacctt tattttgccg tggacattac actgacacta tccatggtag cattgctgcc
     9361 atttactctg cgggaggtgg gaaaatgact tatatcgaaa atatctttct ctgcctcgcc
     9421 ataccgatga tactgtcgct gttctttata aaaggacggt ccagaagcta taccctgttt
     9481 gtgaccattg gaatggggat ttgtctgttg agtgcctatg tcaacagctt ttttatgtgg
     9541 ctatacggcg tggatggaac agttgccgct attgagatca cgcctgtatg tgaagaatta
     9601 ttaaagctgc ttccgttgtt attttacttt ttgatttttg agccggagcc gagagaactg
     9661 cctatggtgg caattgccat tgccgcaggt tttgcaacct ttgaaaatgc atgctacctg
     9721 acggaaaacg gtgcggctaa cttcagcttc ctgctgatcc gggggatttc tgcgggcggg
     9781 ctgcatatcc tttgcgggat cctttccgga ttcggcatct cctatgtctt tcgccaccgc
     9841 tggctgtcag ttaccggaac tgccggaatt ttaggtgcct gcacaggttt tcatggaatt
     9901 tataacctgc tgataacagc cgatggtgga tggaaaacgg cagggtatct ctttccctct
     9961 gttttgatcc tctgcctttt catagcaaaa atccttcttc ctaagttcac catatcttta
    10021 aaataatacg aacaaggcac attgagccgt cccgttttgg ggcggcttaa aattttttca
    10081 aaaatttttg atttttagtg agaaaatttt aaatttcgtg tcttttagta agtagagggg
    10141 tgtgtaaagc atctacaata agggaaggag tgtgacgatg aacggcataa ctgaaatccg
    10201 aatgcgaaaa atacagtcca ggacacggca ataccggcgg cgatatgaat ggcgtgagat
    10261 ctcctgtctg acagtatgca gcctgtttct tttagccgga ataggaattt tgcttgggca
    10321 catacaggaa ccggggatat cttctgtggc agaaggctac ggcgcggttc tgctgcgaaa
    10381 cggagcagat ctgtatgtga ccattggcat cgcctctttt acagccggtg tggtgatgac
    10441 gatcatctgt atccgttgga ggaagaaaaa atggccatac atcggataag gaagaaagtg
    10501 aggaatgaat atgaaaaagc gattgaacag aatgctcagt accctgctga tatgcagcat
    10561 ggtgatgacg ctgatgccca caaccgtttt tgcgacgaac cgtaaggatg ccgttatgga
    10621 agaatacgca ggcggcttgt gcgaacatca tccggaacat gatgatgaat gtggttactc
    10681 tgaaggaata gcaggaacag cctgcagtca tgagcacaca gacgattgct ataaggaggt
    10741 gatcaattgt acccacaggc atgacgagga ttgttacccg gatgaaatta tatgggatga
    10801 tgaggccact ccatccgatg cggaccggtg ggaaccggaa gagtgtcccc atatgtgcga
    10861 ggaggaaagc gagtgtattt cacagaaatt gaactgccgg cataaacatg atgatgaatg
    10921 tggctattcc cccgctacca ggggaacacc ctgcgaattt gactgcgaga tatgtggtgt
    10981 ggatgatgac atgaaaacag ctaccccgtc taatgccaaa gctgtcactg tggcgggcgt
    11041 gcaggctatg atcgacgcac tgccggatgt ggaagatgtc aggaaaggta atgcagaaaa
    11101 agtaaaggcc cagctggatg ccattgacga tgcaaaagcg gagctttctg acgagaaaat
    11161 agatgaactt gatctcttcc gttacatgga agttgctgcc gcattggaac agctgctgta
    11221 tggagtagcg acccagtcta atgctgttat gctggcggat tatgacggcc ctgccatcca
    11281 gcttggtaca ggtgggatca gtggaccgac agaagaagag gaagagggca agggcagcta
    11341 ttatgtcccc aacagctacg tttatttcgg tgtaaacagc ggaaatagca gcacgcctat
    11401 taagtggcgg gtactggatg cggacacggc caacgatgga acaacaagcg gaatgttcct
    11461 gctgtcggag tatttgttgg cgtctgacgt acaatttaac agtaactata gcaagggtaa
    11521 tgcctatcaa ggcagcgacg cacagacttg gtgcagcagc tttgtaggca atacaagcaa
    11581 tttttccacg gcagagcagg ccgttatgtt tggtgttgca aagaccgaca gtggcgaaag
    11641 tggtttgtat tctattcctt ggggcgaaag tagattaact gcaaacgaca agctattttt
    11701 cctgtctgtg cgggaactgg cggactatgt gggaaattat tgtgatgcac caggactggc
    11761 ggcaacaaac accgcgcaga gtgcgggcgt gtggtggctc cgttctcctg actccggcat
    11821 tggttactat acgggcacgg tctacgacga cggtgaagtg gtcaatagcc tcgtgaacca
    11881 cgactgggcg gctcgtcccg cttttaactt aaattcggat tctatcctct tcacatctgc
    11941 cgctgacggc ggaaaaacgg atgctgcggt aaacggtaat ctgaccgaag ttggcacagg
    12001 cagcgcggaa tggaaactga cgttgaaaga tacaagccgc agtttcagcg cttctgcgag
    12061 cagcacattg gtaagggtag gtgaaaacct gaccgttact tactccggcg cgggtacggg
    12121 caagaacgaa tatgtttccg ctatgctcgc agacaacagc ggcaatatcc tctattacgg
    12181 gcgcatcgca cagaacagtg caaatggcac ggcaagcgtg gagattccgt ccgaccttac
    12241 agcaggaacc tataccctga aagtgttcag cgagcagtat aatggcgatt ataagaccga
    12301 ctacgccagt gaattccaga atattacgct gactgttaat gggaaagtca gtgagcagtt
    12361 caatcttacg cctggcacga cctactggtt tgacttatcc agtgcgggga ttccgggttc
    12421 aacaaataac gcattgccgg atacatcccg acactatgtg ccttttactt atgtagggag
    12481 tctcaacgct tatgtgctga aacctgcatc tgaaaatgtg ctgacctcct ccgaggaagc
    12541 ttcccaagtc acagataaag atgcaaagta cggatatact tacaagcaca gtctgttcat
    12601 tgccgactat actatgaccc ataccataag ctggaaaatt ctaaatgata acaagctgat
    12661 ttttggcaca gactttcaga acaaccgtgt ggcatacaca ctgcgtgcgc catcagcagg
    12721 acacacctat gcttatggtg ataatggcgg cagtacccca gacaacaacg agtgggacac
    12781 aattttgaag aaagccaatc aaagtactca aaccaatgta agcgattata tcaaaaattg
    12841 ggaaggcatt tggtcttatg ggcaggacac tgcgaataca tctgccttca ggcagtttcg
    12901 tacaatacgc ggacgtatga tatctgcgtt ctcctgtaat atggaacata aagcagcatc
    12961 gctcccaaaa tacggtttcc gtcctattct tgaaattctg gacgctgata cgttgggcta
    13021 tgacggactg aaaaccatag tgcttgactt aaatgatggg aaaattggcg ataccacagg
    13081 cacggtaaat atcgttgtga aaaacagtga gagcttcacg gctcccgaaa gttccggcct
    13141 gtccagtcca atcgggaaaa cggatactga tttctgctgg cagggcagcg atgggaattc
    13201 ttatattcct ggtgcagatg ttcctgcagg tgtaacttcc ctgacggcgc aatggacacc
    13261 tatgacttat accgtaatgc tggaaacaaa cggtggtacg attgctgacg gaaaagatgt
    13321 gataagctat acctatggcg aaggagcaac cttgccgaca gcaaatgata tgacccgaga
    13381 gggctataca tttgatggtt ggtatgcgga cagcagtttt tccagcgctc ctgttatgga
    13441 gatcagcaac actgacatag gaaacaaaga gttctatgca aagtggaatg cgaacatcta
    13501 cgcagtaacg ctgaacccca acggtggaac gattgccagt ggaaaggaca taacaagcta
    13561 tacctacggc aatggtgcaa ccttgcctac atcaaatgat atgacccgcg agggctatac
    13621 atttgaaggt tggtatgcag acagcctatt ttccggcgct cctgttacgg agatcaacag
    13681 caccgacatg ggcaacaagg agttctatgc aaagtggacg cggaacacca cgccaattat
    13741 ccccggcgat acggtaaact atattgtgga gcattacaag gccggtaaca atggttatat
    13801 gctggaagaa acagagaaac ttggagacaa aatcggcgcg aatgttaccg ccgagccgaa
    13861 aatctacaca ggctacactt ataacccgga tgtagcggga agtgttacca gtggtacatt
    13921 aaaaaagatc agcagtgcgg cagatattgt gaccttgaaa ctctactatg acctgactgt
    13981 ttacaacgtg actgtagaaa acgatgggaa cggatctgca tctgctgctc cggcttctgc
    14041 aacgatgggc gggaaaatca cgctgaccgt aactccaaac agtggctatc gctttaagga
    14101 atggcagatt gtttccggca acgtgacgat cagtgatgat acgtttacca tgccagcagg
    14161 taatgtgact gtgaaagctc tttttgaaag aaaatcgagc agtggcagag attcttcctc
    14221 tgaccgtgat acatcctctt ccgcaatcag aaaagatccg atcaggggat ggaccagcag
    14281 tgatcgcggc atcattaccg gcgcagccaa tagcattgcc aatgacggtt acagtcactg
    14341 gatgcaggat gatcatggct ggtggctccg ttttgcggat aacagctatc cgaaaggcca
    14401 aaagcgtggt acgtccggca ctgcatatgt ctgggaactt atcaatggca gttggtgggc
    14461 atttgacgag aatggttatg ctaaaatcgg ctggctgagg gatgatacat ttggcggctg
    14521 gttctatatc gatccggaac gaggtatgca gactggatgg gttcggcttg gtggtgcctg
    14581 gtattatttc caccaggtat ctgatggtag aaagggcatt atgtatgccg gacggaaaac
    14641 accagacgga tattatgtag acgaaaatgg cgcgtggatg gcaaagaaaa ataaatcagc
    14701 cggaatataa aatggtcaga ggtgcgcatg aaaagagtta atatcatata gggcacgact
    14761 tatagtaata tattcctgga atattttcat gaagcattaa ataatttatt tgacgaacac
    14821 acattatttt tatatgaatt aatgtgtgtt cgttattttt aattaacatt tttcatatct
    14881 catacaaaaa atgaagatga tcatttgaaa cacagatata aaggcatttc gaaaggaaaa
    14941 tccccggcat tttccaggaa aaacagcaga aaatgtaagc gtttacaaaa tttatgtaag
    15001 tatttacaat tttgtatttg aaaagtagaa aaagtgtggt atactgcaat tcagagagga
    15061 catttccggg gtttcggaaa ggaaatggtt tccggaaaat tccggaagag tccagaaagc
    15121 gaaacaggaa acaatatcag ggaatgatcc gtataggaaa ggagaatatt aaattatggc
    15181 acttctgaat tatgatgtat taaagaaatc cggttacgat ggctatatcc tggaggatgc
    15241 tccggtgaag gttttacagt ttggtgaggg aaacttcctt cgcgcattcg tagattactg
    15301 gtttgacctt gcaaacgaga aagccggatg gaacggcaaa tgctgcctgg tacagccaat
    15361 cgcaccaggt ctttcaaaga tgatcaatga gcaggaaggt ctgtacactc tgtacctgcg
    15421 tggaagcgaa aacggcaaga aggtagatga caagcgtgtg atctccagcg ttgctaactg
    15481 cttaaatcca tatgagaaag ctgattacga taagatgatg gaagttgctg caagcgacga
    15541 tctgcagatc atcgtttcca acacaactga ggctggtatc cagtatgatc catcctgtaa
    15601 gaaggatgac tgcccaccgg caagcttccc gggaaagctg acccaggttc tgtatcatcg
    15661 tttccaggct ggtaagccag gaattatcat gctggcttgc gagctgatcg acaacaacgg
    15721 taaagaactg ttaaagtgcg taaaccagta cattgatcag tgggaactgg gcgctgactt
    15781 caagaagtat gtaaatgaag actgcatttt ctgtggttcc ttagtagacc gtatcgttcc
    15841 aggacgtatc cgtgacgcag aagaagttaa ggctctggat gcaaagaacg gctacgaaga
    15901 tcctctgacc gacgttggtg aagtattcgg cgtttgggta atcgaaggcg atgagaagtt
    15961 aaatgacgta ctgccattta agaaggctgg tctgttagac aaggtattcg ttactccgga
    16021 tatgagccca tacaagaaga gaaaagttcg tatcttaaac ggcgctcata caggtttcgt
    16081 tctgggtgct tacctggctg gcgagaacat tgttcgtgac tgcatgaacg atgagaccat
    16141 caagggcttc atgaacaaga tgctttacga tgaagttatc ccaaccctgc cactggacaa
    16201 gaacgatctg ttaaactttg cagcagctgt atctgatcgt ttcaacaacc catttgtaaa
    16261 ccatgaactg atgagcattt ccttaaactc cacctctaag tggaaagcaa gaaatatgcc
    16321 ttccttcctg gaatacatca aggaaaaagg tgctctgcca gagtgcctga ccatgtcact
    16381 ggctgcatac atcgcattct acagcaatga tatccagtct cttacagatg caggtctggt
    16441 ttgcaagcgt ccggctggca acgagtacac tgtaagtgat gaccgttggg tactggaatt
    16501 ctacaacgat cacaaggatg cttctgcaga agaactggta cacgcagttc tgaccaatga
    16561 aaagatgtgg gatcaggatc tgacccagat cgaaggtctg gaagctaagg ttgcagctga
    16621 cctgaagctg atccgtgaaa aaggcgctaa ggctgcatat gctagctgcc tgtaaaatgg
    16681 aagaatatag gtcttatttg atataaaatc tatacattat ttaagaaatg aaggggctgt
    16741 ccgaaaatgg gcagcccttt ttgtaaggat ttcagaatac tattttatta ttccggtttt
    16801 tgcgtattcc cccagttttt ttctcagcac catatcctgt ggatctaaga actgccgcac
    16861 tttatcatag agcacttcat ctggttttaa cgccgcccgg tactgggcgg gagtgcgctg
    16921 gaaggtggaa cggaaacggg cagtgagagt ctttagatca ggaaagccat tttttaatgc
    16981 aatgtccgtc aggttatccc tggtgtaggt caggtcattt aaggcatgct gaaatcttac
    17041 tcttgtaaga tattcgtgga aattgatacc taccatttgt ttaaatagag ttgaaacata
    17101 ggtccggttg tactgggcgt agtctgccag atcctccagg gataattttt ctgcataatg
    17161 ttcctctaca taactgagga gaagatggat cgtagcgcgt cgtttgtctt cttcagagat
    17221 cagattgatc tgttggggat caaattctgt aaaaagtgtg ataaggagca gctccaggtt
    17281 tgcttttgca cttaactggg cataggtacc gccacattct agtgactgcc agatctggct
    17341 ggcataaaag cggatgcggc agaaacgttc ttcgtaacgg ttgttttcat tggaaagaca
    17401 ggagggaaac tggtaaatat atcccttttt aactaaaagt tttaatgcat ttgacggaaa
    17461 atgcaggacc agagcccggg tgtcaggctg ctggcgcatg gaggcgtggc cgttgcctgg
    17521 tgctgtcagg ataaggtcat cttcaaaaag agagtgtgtt tctgtaccac ggcagtattc
    17581 ctggttgcct ttaagaacga tgctcagctc atattcatcc gggtgccagt gataacggta
    17641 attcataatg ctgtgtgcaa agcagcgcag gtggatattt tcacggggca gatccatttc
    17701 ataataagtt tcttccatag gggctccttt ttattctcta aaattttatt tttcaaaatc
    17761 caaacatttt ccctgttgta aaatttagaa aagatacgat ccagatttta aacctgattt
    17821 cagaatactg tttaatgtgg cctgtgtcaa cgattgtttt tccttaaata caaaagaaaa
    17881 accttggaaa ctggcagaat gcacaagaat caggacaaaa aaatgacgaa ataaacaggg
    17941 gaaatatttg gattttttac ggtaaaagac agtagggact gagggcagaa gaggaaaatg
    18001 gaaaacagag tgcagtaaaa agctgcgaat attcacagcg acaaaaaatt cacattctgt
    18061 taggatgagc ataacaacaa tgagccccgg cccgggcagc taaaagctta tggagttttc
    18121 agactataat ttaataatga tgtaataatg gaagggaaaa gaatatgaat actcaaatga
    18181 tgatctgtat ggtgatcttt gcagctactc tggtcagcta tatgatgaat aagataccaa
    18241 tgtggctaac agcaatgctc tctctgactg cactgtattt tacaggctgc attgatgcaa
    18301 ctggcgcact ggctggtttt tcaaatgcta atacgctgct tatggcaact atgtttatag
    18361 tagcggcagg tttccgccgc acatctctgg tagatggaat gtgtgatgct attatgaaat
    18421 tgacaagagg aagttttctc acagcctatt ttggatatat cctgattgct gtattactga
    18481 ctaactttat tgccagtccg atggttgtat atgcgatcgt aagtcccctg ctggcagctc
    18541 tttgtgacaa cactggtcac agccgtaccc agtacatgtt cccggttatg gttgtatgtg
    18601 tggcatgctg cggcatcctg ccgctatcta ctgctatcca gcaggcagga cagtttaccg
    18661 gatttatgga aacctacgga tttactgata tgagtatccg tgctgtagat tttaccattg
    18721 gtacctggcc gatccttatt attgttcctc tctgggcatt atttattgga ccgaaattca
    18781 cacctaaaac accggtagtt cctattgaag ccctggaaaa gagaaatgct ggaaaacagg
    18841 agcagaaact cagtccggtt gtagacaaga tcggaatcgc aattttcttt ggtacgatct
    18901 tatgcctcat tttctccagc cagttacatc ttgctacatg gtctgttgca ttgaccggaa
    18961 gccttcttat ggtattattt ggtgttgtag accagaaagc tgcgctgcgc gacattccgt
    19021 gggatatgct gatgttgttt gtaggcgcac tggctctggg aacagcactg acaaataccg
    19081 gtgcaggcga tatgatcgga aatacccttg catcagctgt tggtggaaca cataacaatt
    19141 atgttcttgg cgcactgttt ttcctgatcc cgtttgtact tactcagttt atgctgaacc
    19201 gttctgtatc tgcagtattt gttccaatct gtctgcttac aagttctgcg ctgggcgcaa
    19261 accctgttgg actggtactg ttagttaatg caggttccct gactgcattt ttaacaccta
    19321 tggcaactcc ggctgttcct atgtgtatgg cagatggagg ttatgactta agggcaattt
    19381 ttaagagcgg tgccttgatc acattgatcc tgccatttat ttatatcttt tatactatga
    19441 ccgtatttcc ggcattttaa agagaagaag gagtggatat ggctatgacc caggaagaaa
    19501 aaatcgaaat tgcggatatg gtacgcagcc gtgatcggtt tggtccggta agcgaagaag
    19561 aacttgcagc gctgccggga aaagaaacag aactttggat ccgcacaaaa aacggaaaaa
    19621 cggtgcatgt ttttgaagaa cgtccagaaa atttaccaga acaggctgca ctgctgttaa
    19681 attttcatgg cggtggattt ctgaaaggcc gtacagaccg ggaccgcagg tattgctgct
    19741 gggccatgga acaactaaat tgcctggtat gggatattga ctacagcctt gcaccggaaa
    19801 atccatttcc ttttgcggca gaggaagctt atgagatcgt aagttatgca tttgaacata
    19861 gtacagaact tggaattgac caggaaaaga tcattcttgc cggacatagt gcaggaggga
    19921 atttagtagc atctgttctg atcaaaaatg aggagacgaa agaactgcat ccttgttgtg
    19981 cattaatgga atatttccct acagataata ccgtagatcc agtagaaaga ttgtcagaag
    20041 aactgaagaa agatccattt tgggtaaaac gtgcggagac agaaaaaatg tacactgatt
    20101 tttatgtagg agatgcagat ccatcagatc ctttgtgttc acctgcattt gccaaagatc
    20161 aggcattggc cgcgtttcca gactgtctga tcatatcagc cggggaagac agcctcagag
    20221 atgatacaga agcatttgga atgcgtctgg caaaagccgg agtctgtgtg acaatgcagc
    20281 ggatcacaga ggctatgcat ggatttacta caaaccggac acctggctgg gaacgggcac
    20341 tggatcgtca gtacaaattt tttcaggagc atttaagata gaacaaatgt caaaagaaaa
    20401 gatattatat gaggagtaag acaatgaaga ttacagatgt aaagctgcgt tttgcaaagc
    20461 attacctgtt tgtacagata tatactgatg ccgggatcac aggtcttggc gaggctggta
    20521 actgggggta tttacaggcc acagcagcag ccattgaaaa gtttgcagat tacctgatcg
    20581 gaaaagatcc gttccgtatt gaggatttta accagaattt cctgcgttcc gtgtatttcc
    20641 gcggttctgt tattatgagc gctatttctg ctattgatat tgcactgtgg gatattaagg
    20701 gtaaggcact tggagttcca gtttatgagc ttttaggcgg aaagacccgc gaaaaagtcc
    20761 gcgtttatgc atctgttatg catctcacag aagataacaa tgagcttgca aagcagtatc
    20821 agcagttgca ggaaatgggc tttacagctg caaaaatctt ctgcaacggt ccggtcagcg
    20881 caccagatgg aaaaggggaa ttttattcca gccgtattga acgtgaagta gaaaaggtac
    20941 gtgtctgccg tgaagcagtt ggaaatgatt ttgactttgt actggaagtg catcgtggca
    21001 tgactctgcc ggaggcagtt gcatttggac gtgcagtaga gccttaccgt cctatgatct
    21061 tagaagatcc ggttccgccg gataatgtag ataccatggc agaggttgca tccaaagtgg
    21121 gcgttcccat tgccactgga gagcgcttta tcgacctgcg tgaatttgag gtattaatgg
    21181 cacgtcacgc ctgccagtat gtccgcccgg atgtatgtgc agtaggcggt atcactacca
    21241 gcaagaagat ctgcgcgctg gcagaggctc atgacgtcct tgtgatcccc cacaatccac
    21301 tgggacctgt atccacagca gcatgcctgc agatctgtgc ctccattcca aatcttggca
    21361 tccaggaact gcctggtttc tgcttaaacg gtgcagaaga taagatggta aaagaaccgc
    21421 tgcgctttga aaatggatgc atgctgatcc cggaagcacc tggtattggt gtggaactgg
    21481 cagatgatgc agaaggactg tatccggcca atgagcgtgg cagcaatgca gcccgcaggg
    21541 cctttgacgg gtctgtaaaa gactggtgat aacataatat gataccaggg tcaaaaggtc
    21601 agaaatccga cgcaagcttg cttgcagtag gatttttgaa cttgggaccc gccaaaacat
    21661 gagtaagaag ccattttttg aagaaaaatg agcggattac gaatgttttt aggattgcgg
    21721 gagtgcaatg cacgtagcaa tccggtatca gaggggacaa atacggctgc aatgggaata
    21781 attctcgttg cagcctgttt taataaagtt catttacaga tctggtctgc tactgcctgt
    21841 gcaaacacag cgctggcaag atacagaacg gagtcgtcaa tgcagaaacg gccattatgg
    21901 tgtggatatt cctttccatc atcagaagga atggaattat acagaaacgc ataacagcca
    21961 ggcacttctg taaagaagta ggaagagtcc tctccaccca tggaagaatg atccagttcc
    22021 aggatatcct ctgccgggaa gacttttttt gctgctgtaa tgaaatcggc agtcatagtt
    22081 gggtcattga tcaccggagg gcagccggac ggaactttca tttcagcagt tgcgccgtac
    22141 atggcagcac actgtttact gatctcttcc atacgtttta acaggtggct gcgcacttca
    22201 agactctggg tacgaagacc gcctacaata gtggcagaat ttgggataat gttataagct
    22261 ccatttccgg cagtaatacc ggtgactgtt aatacagcag gtacattata ggtggattct
    22321 ctggatacca gtccctgaag gctgtttatt atttcagctg cgatcaaaat aggattgatg
    22381 gccagaaacg gggtggaagc atgtccgcct tttccgtgaa tatggatctc aatacagtcg
    22441 ctggaagcaa aaacagcgcc ggttttccag aaaagctgcc cggattttgt gccgtgttga
    22501 agggtccgga tggaagcagc tgctttctgt ctggactttt ttttatattg tgttatataa
    22561 tggacaaagt gacattttgt ccggcggtac atctgtgttg gcagaagccg ggtgcagttt
    22621 tatgcaaaag aggtaatatt ttatgtatca cgatctaaca aaggggaaca tttcccgttc
    22681 ccttctttta tttgctctgc ctatgatggc aggaaatctg ctccagcagt tttacaatat
    22741 tgcagacact ttgatcgtag gccgggtgct gggaagagat gcgttggcgg cagttggttc
    22801 tgcttataca ctgatgactt ttcttacgtc tatctttctg gggttaagtt taggttccgg
    22861 ggcgctgttt tctatttaca agggcagagg ggatgaaaaa acattaaaat cctctgtatt
    22921 tcatgccttt gtgctgatca tggcagtaac agtagtctta aatatcctgg tctatctggg
    22981 actggatggc attctggcat ttttaaaggt gccggaagag gtatggcagg gaatgcgcca
    23041 gtatctgttg atcatctttg caggcctgat cgcaacatcc ctttataact tctttgcctg
    23101 tctgctgcgg gcaatgggaa attctacggt gccgctggtg tttttggcag tttctacggt
    23161 tattaacatt gtactggatc tgctgtttgt ggccgtgtgg tctatgggga tcggcggtgc
    23221 ggcactggct accattatcg cacagtacat ttcgggactt ggtatcacgg ttttcgccct
    23281 tcgctgctgt ccgcaggtga tccccaaaaa gagtgagctt aaatggaatg gcggcatttt
    23341 aaaagagatc gtcaacctgt cttctatgac ctgtatccag cagtcggtaa tgaatttcgg
    23401 catccttatg gtccagggac tggtaaacag ctttggcccg gtagtcatgg cagcttttgc
    23461 ggcagcggtg aagattgact cctttgccta tctgccggtt caggattttg gtaatgctta
    23521 ttctaccttt attgcgcaga actatggagc aggaaacaga gagcgtatca aaaagggcac
    23581 aaaacaggct tttgcactga gtattctgtt ctgtatcttt atctctgccc tggtatttat
    23641 ctttgcagga cagttaatgg gcattttcgt aagcaaggca gaaacagagg ttattgcctg
    23701 tggtattaaa tatctgcggg tagaaggtgc attttactgg ggaattggct gtctgttcct
    23761 gttctacggt tattaccgtg ctgtaaagcg ggcgggaatg tccgtagtcc ttacggttat
    23821 ttctttagga atgcgtgtac ttttagctta tgtgttgtct gcgaaagtag gagaagtagg
    23881 tatctgggcg gcgatcccca tcggttgggt actggcagat attacaggac tggtttgtat
    23941 gttaagatcg aaactttaag acatgttgaa aaggcaaagt ccaggtggtt tggaataacg
    24001 gaggcatggt tgtttggata aaaatgagga cagaacgatc aaaaacaagg tcagagaaaa
    24061 gatacaggga gtgtccatgc tcccttttgg actgatcgcc ggttttgccg gtgaaaaaga
    24121 aattcgtatc atagagcttg cggaaaacgg ttttaccttc cgcacagcag aaaaaatagc
    24181 agacccggaa ttatttcgag tctgctttta tcattatccg aaactggagt atgagcaggt
    24241 agaactgcat tcttatacac tggaatgcag cggagaatat gaattttatt atgagtatac
    24301 tctatatctg gatcatataa aaaataaaga tattatgtgg gacagtcatt ttatagatga
    24361 taatgatagt gcagaattta tagaataccg caaacaggta cagtatcttt tagggtggta
    24421 tgaccggtat atccggatga agttactggg agacagtgaa gaactggcaa gttttatgac
    24481 agcgtacccg gaaggcgggg atgtggtcac tgccagggac tttgaagagc agaaaaaagc
    24541 ctggtttttc agagagacga aagaaattca cagggatgaa aaagaagtag aagcagaaaa
    24601 agcaaagaaa acagtacagg atgcaaaaca gaaattgaaa actggattgg aggttggctc
    24661 agaaagtccg gtatctgagt atgcattgga gttggataat ccctggcttt acgaggaata
    24721 tttatccaag gatcttacaa ccttcatgga aaattactgg aagcgaaatt acctgtccgg
    24781 gcactggctt tccgatcact ttccacagcg tctttacatc ggaaattcct tctgccacct
    24841 gctttttcca aaggaagacc agttgtttca gcttcttgaa aaagccagaa aagagagctt
    24901 acaggtaact ttaacatttt cctatatccg cgaatttatg ttagaaaaca caggtcagct
    24961 gatggaaaag ctggaaaatt ggtgcgaaga aaataaggtt actttggaga tacaggtaaa
    25021 tgactgggca atggcagaaa tgttaaaggg gaaagttttg tttgttccgg tacttggaat
    25081 tttattaaat aagcggcgga aagatccaag actgcactat aaaacagaaa caatggcgca
    25141 gatcggggca gagacggaaa gtaagttcct tgaagaaaac agcttaaatg ccgattttta
    25201 caggaaatat ctggaaaaag aatacggtat tgtaaggtat gagtgggaaa gctgcggcta
    25261 tgatatggct ctgccaccag gaaaaaacag cctgcatctt cctttttacc agaccaatac
    25321 ctcccagtac tgcacattgt atgcaaagtg tatgacagga gagcggggaa agcagaagct
    25381 ggcagaggaa tgtccgaagt tttgtaaaga ctatgcattt ttgtatccgg atcatttgat
    25441 gatgatcggc agatataatt cgttatttgc actggacagt cgtatttttg aggatggttc
    25501 tggttcagga acaggaactg aaattggaac aaaaataaaa acagaagaaa tattgaaaaa
    25561 atggaaaaaa gcgggtgtgg atcgtctggt agtgaacctg cttgatccag gtgttaaaag
    25621 ataaaaaaat aaaagtacat gatcaggaaa ggagtgaaaa cgcagatgaa gatcgtagcc
    25681 ggacttggtt ctatagatga atatatcccc tatgtaaaag ccggggcaga cgaatttttc
    25741 tgtggctacg tcccttattc atggacggaa aaatatggga cacttctgcc tcttaaccgc
    25801 agggaagtcc tttgttccaa tgtacagtta ggcgcgttca gtgaactgga aatactggct
    25861 tctatggtaa aaaaatacga aaaaccagtc cacctaacct ttaactcttt atactacatt
    25921 ccagaacaat acctggaaat tgctaatatc attctacagt gtatgtctat cggctttcaa
    25981 agctttatca tcgcagatcc ggcgctgctt ttatatctgc gccagcagag gatagattgt
    26041 gatattcacc tgagtggtga aacagcagag gtcaacagcg aaatgctaaa gacttttgaa
    26101 aaacggaacc tgaagcgtct gatctttcac agaaaaaaca gtatagaaga tatgggatcc
    26161 attatcagca aagcaggaca gaccacagaa tttgaagcct ttgcactaaa tgaaatgtgt
    26221 cagtttaccg gtgcattctg caattctctc cactgcgatg aaatgtgcca tctttgtctg
    26281 gttccctatg aattggggcg gatcagagaa tgtgtatcag cagaaaacag attgaaaaca
    26341 agcccattgc cagtgaaact ccaggaaaat gaaatgaaaa acattagatc agcagaaaat
    26401 gtggatgaac cggaagatga cagctatctt tgtggacaga ctggctgtgg tctctgcgct
    26461 ttataccggt taggaaaagc tggtgttact catttgaaac tggtaggaag aggcaattac
    26521 acagattata tggaacgtga cattagaaat cttcgaaagg cactggaaat attaaaagat
    26581 gtcctagata tggaaaaaac tggaaacatt ccagccggat taaaagcgga agaaacatat
    26641 atctctcaaa tgaaaaagga actgtttggt tcagctggaa aatgtagtgg aatgtgttat
    26701 tatatgaatg tgctatcctt ttgcaaaaac aacagaagtg gtgaacatat aaaacggagg
    26761 aaataatcat gcgctatgct tatgtaggat gccagacaac gaaagagaga aatgcccggg
    26821 gagaaggtct taaaacttat gaaatccagc cagatggcag ctggaaatgc ctgcagtgtc
    26881 tggaaacaga gccaaacccg tcttttcaga ctctggatgg cgaaggaaaa tatctttaca
    26941 gtgttcatgg ggatattacg aaggtttcca gttaccgtat tttaaaagat catacattag
    27001 agcatataaa taccattgat attggcggca gaaatcctgt ggatataaca gtggaccgca
    27061 acaataaaca tgtaatcgtt gccactttac agggaggtac catttacacc attgaccgga
    27121 agaatgacgg ttccctggga gaagtgatcg cagaatttac ctatgagggc aaggaaccgg
    27181 gaaaggtatc aaccattcat cagtgtcttt gggataaaaa gaagaacttt ttatttgcct
    27241 gtgcccagag ccgtgtaaat ggttatggac agatgcgggc cttacgttat gatcatgaga
    27301 ccggacagtt tactcagacg gctcagtttt tatccagaac atgggatgag ccccgtcacg
    27361 cagcagtgca cccaaataac ctctgggtgt atatgtgtga ggagaaggga aataaagttc
    27421 ttttcttccg ttttgatgat gaaaatggta cctttgaggc gttgcaggag ctgaccactg
    27481 tgccggaaac agtgactgga tattccgatg ccagcgaggt catggttgac ccttcaggca
    27541 aatttgttct tgtatccaac cgttatacag acagtattgc agtctaccgt atcgacccgc
    27601 tgaccggtta tatgcgaaac gtaggatttt acccatgcct gggaaaaact ccacggttct
    27661 tctgttttgg agacaatgga aagtgctacg ttgcaaatga ggacagtgat accattatag
    27721 aatttgattt tgacaatatg accggggcac tgatcccaac attgaatatt gtgcataccg
    27781 gcagcccggt gtgtattctg tttgcagatt aaacaaaaac tccccctgta tctttgacgt
    27841 acagagggag tttttacact tttacggacg taatttgtat tacatcagtt ttttaccatc
    27901 agtaaaggca tttccaacaa catcacctaa aacaacgtac ttgttgttaa atgggtcaaa
    27961 ggtgatcggc tgtgcttttg cgatgtctac atttccgttt tcatctaata ctttctcatc
    28021 aacactgaca ttgacgatct cacctaccag acggcaggtt ttggcatcat agcttttcag
    28081 tctgcattct acagcaactg ccagctcttc aataagagga gcatcaacaa actccgattt
    28141 tacagcatgg aaaccggctt tttcaaattt atcaggtact ttgtttgcag aaacaatgcc
    28201 tacgtagtcg caggctgtca tatgaggcgc atctgccatg cttacagtaa aggctttgcg
    28261 ggctaaaagg ttctttgtgg ttttgtggcc ttcgctgatg cacatggaaa gttcatttcc
    28321 ttcactgatg ccgccccagg ctgcgttcat cacatctgga gtgccgtttt catcgtaagt
    28381 tgcgatcata aatactggct gtggataggt gtatggtttt gctccaaaat tctttctcat
    28441 gataaattct ccttattatg atagtgtagt agattttaaa atagttccaa attcgtttat
    28501 tcccaggtaa gttttcctgt ctttactgct tcctcatagc gggagatctt gaaatccagc
    28561 cgcttgatgg tgctttcaat ctgtgctttc tgctctaata aggtttccat ctgctccttt
    28621 aaaagaagca ttctggcttc gatggtggaa tctccctgct ggcacagttt tacatattcg
    28681 atcatggctt ctacaggaag tccggcgcca cgcatacaca gggccagttc tacccagcca
    28741 agatcagact cgctataatc gcggatgcct ccggcagtcc gggtcacagg tgggatcatt
    28801 cccacacgct cgtagtagcg cagtgtatca ctggaaatat tgtatttctg gcttacttct
    28861 ttgatggtca cggtttcaca ctcctttttc ataaaagtat agttttctgg tctgaaaaca
    28921 ttatacaacc tggagctgac tctaagtcaa gatatacagg caaagtggaa tgttaattgc
    28981 cgcaaagcag aggcaaaatt acttcttagg ctgataccgt atatcagata ctgtatatac
    29041 agtaataaat gcatgtgggt cttcacgatt gatataattc atcagtttct ggtactccga
    29101 tttgtctaca atagtaatga tctcattgcg cttttccata ttgtaggcgc cgatcgactc
    29161 atagatcgta gcgccgctgt gcaggtcatt gatgataaaa tggcgcagtt cttcttcttt
    29221 ttttgtgata atgcagaccc ggcgctttac attgtggtta aagataaaat gatccaggat
    29281 gatgccgtta aaataagttc ccagtacact taaaacaacg gttttcttgt catacaccaa
    29341 agctgctgaa agggcaacgc acatgccgga taaggacatg gccttcccaa gatccatgtg
    29401 cagatatttg ttcatgatct tggcaacgat atccagtccg ccggaagaag catttcggtt
    29461 aaacaggatg cttaaaccga tactgactgt taaaatatag cataaaacat ccagttcctg
    29521 actgtcagtt aaggagccga tgttcgggaa gatcctttca aaaagggcca ggaaaagggg
    29581 aagtacaata ctggtgtata cggttttagc cccgaattct tttccgcagg tgaaaaatcc
    29641 gatgatcagt aaaagtacat ttaagatcat ggtgatagcg gaaaggggca gaggtacaaa
    29701 gttagataag ataataccaa gaccggagat actgcttacg gaagcatggc ttggtaaaag
    29761 aaagaagtac acagctccgg ctacgatggc catagcggct gtaaggatca gtgtttcttt
    29821 gatcagttct gtaatatggt tgtttgaatg gttcatgaca cacctcttta gagatatatt
    29881 tggttatggt tcagctatgg ttgaacggta actgtatttg caacattcat tttaccatag
    29941 cttgattgtt ttagaaagtg gggatatact tttagcagaa agggtttcac ttacaaaaat
    30001 cattttgcga aaagagaaag gaatatgaac attcaggtaa gagcatatac agaaaatgac
    30061 cttccggata tgatccgaat ctggaacgaa gtagtagaag acggtgtggc atttccacag
    30121 gaagaatttc ttacagaaga gacaggaaaa agtttttttg ctgagcagac atacacagca
    30181 gtagcagagg atgcagaaac aggaaacgta tacggtcttt acatccttca tccaaacaat
    30241 atcggccgat gcggtcacat ctgcaatgcc agctatgcag taagccggga ctccaggggg
    30301 attcatatcg gtgaaaaact ggtaagcgac tgcctgaaac agggaaagga acatggcttt
    30361 ggcgtactgc agtttaatgc ggtagttgcc aataatatcc atgcaagaca tttgtatgag
    30421 cgtctgggat ttatccagtt aggtgtgata ccaaagggat ttcgcatgaa agatggacat
    30481 tttgaagata tttgtccata ttatcacgaa ttataagaag acagaaataa agcaaaataa
    30541 agaaaaacaa aaggaaaata taaaagaaaa gataagagcc agagcgcagg atcaatgaca
    30601 agacctgtgc tctggctttt cgtatttaaa aatcccccac tttccagaga gttacatggg
    30661 gcgtaacttt aatgaaattg tggaatatgc gggatgggcg tatgataatg ataacaaatt
    30721 tgttgaattt ttatcatgta cggtacagaa aaaggggtaa aaaatggtgg aaacaagtaa
    30781 gaggctgaaa ggtgccattg atatgcacat ccattcagcc cagatgtacg gcagagaaaa
    30841 aacaatgacc tggaagatct taaaagctca taaccatagt aatacaatgg aagatctcca
    30901 gctggtcttt gacgctttag tatctccaga taataactat gtaagcatcc ttcagactgc
    30961 cagagccagc ggcttaaaga aatttccagt tccctatgta ttgtctaact gccacaacag
    31021 cctttgcgct gttggcggca ccattaatga ggatgatcat gtatttggac tgggaaatgc
    31081 tgtaaagtac ggcggtatct ttgtgccccc atataaggca gtgatccatc agtatatgcg
    31141 tgaaatgatg gcaggctgtg gaaaaatgat ccttggttct gacagccata cccgctatgg
    31201 cgtactggga acaatgggca ttggcgaagg cggcggtgag atcgcaaagc agcttttaaa
    31261 gaagacttac gatatcccat atccaaaggt catagcagta aaactgaccg gtgcaccaaa
    31321 gccgggagta ggtcctcagg atgtggcact ggcactgatc gcagctacct ttaaaaatgg
    31381 atttaacaaa aataaagtcc tggaatttat aggagacggc attgataacc tgtctgtgga
    31441 atacagaatg ggtattgatg taatgaccac agaaagtgcg gctctttcca gcatctggtg
    31501 tacagatgaa aaagtccatg aatatttaaa gcttcatgga agagaagaag agtacaaaga
    31561 aatgatgcca gaagcagggg catgttatga cagtttaatt gagatcgact taagccaggt
    31621 agaatgtatg atcgctcttc cttatcatcc aagcaacgca attcctatct cttattttaa
    31681 agagcatatg gaagaactgt taaaagaagt ggaagaggaa ggccggaaga tcaaaggaga
    31741 caatggaaag ccgttttcta ttatgtccca tatacatgac ggcggttttt atgtggatca
    31801 ggctctggtc agcggctgca gcggcggtat gtttgagaat atttccgcta tggcagatat
    31861 tttagacgga tattccattt ctggtgatca gtgcagtctg ggcgtaaacc cgtccagtat
    31921 gcctattatg gcagaaatga tggatcaggg cattgcaaaa cagctgatgt taagtggagc
    31981 gaccatccgt ccggctatct gcggtccctg ctttggcgta acagatgtcc ctgcagataa
    32041 tcaggtaagt atccgccaca ccacaagaaa ttatccaaac agagaaggct ccaaacctgg
    32101 aaaaggccag atggcagcag cttttcttat ggatgcccgt tccattgcag caacggtgcg
    32161 aaacggtgga agactgactg cagctacgga actggaggta gaatacacag acaggaaagc
    32221 tggttttgac cgttccattt atgaaaaaca ggtctacaac aattacggca aagaaaagcg
    32281 gtcaacagaa ttaaaaatgg gaccgaatat tgcagactgg ccggaaatgt ttcctctaaa
    32341 gaaacacctg cttttaaaga cagttggcgt atatgaaggt tctcttacaa ccgatgagct
    32401 ggtgccttca ggagatgctt cttcctatcg ttccaatccg gaaaaactgg cagaatttac
    32461 tctctgcagc agagacccgg agtatgtaaa gcgggctatg gcagtacgag aaacggcaag
    32521 acagcaggaa gcaggacaga agctttctga ccaggaaatg gatggtctgc tggagcagat
    32581 cgctgcaaaa tacggcgcag atccaaagga gatctccata ggaagtcttc ttgcaggagt
    32641 aaaaatagga gatggttctt ccagagaaca ggcggccagc agccagaaag tattaggggg
    32701 ctgggcagat ctggcagagg aatactccac caaacggtac cgctccaatc tgatcaactg
    32761 gggtatactg cctctgatca caaaagagcc tctggtggtg aaaaacgggg atgttatctt
    32821 gatccaggat attgaaaccg ttcttaaaga tggcagagaa gaactgactg cttatctgtt
    32881 agatgaaaaa acaggtgaga caaaaaaaga gataccgtgt tccttaggac ggcttacagc
    32941 tgaagaacgt aagatccttc tttcaggctc cttgataaac cattataaat aaactatact
    33001 ataactaaat atgcagaaaa tgcagtaaaa acatccaaaa ggggtgcctt ctatgacaaa
    33061 tgaagatctg gaaaaagtga tcg
//
LOCUS       DBMDUA010000009        30483 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1124_length_30483_cov_2.685439, whole genome shotgun sequence.
ACCESSION   DBMDUA010000009 DBMDUA010000000
VERSION     DBMDUA010000009.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 30483)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 30483)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30483
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1124_length_30483_cov_2.685439"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="ACZPCL_01515"
     CDS             complement(<1..620)
                     /locus_tag="ACZPCL_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161202.1"
                     /translation="MKWIKETKGKRQAAIGAVFLVVFLSLVMALSCAEFYKRYKEQVI
                     HTEESQLLTIAGIIGNNLDMFLNEQLEQIDLFYSSENGLLGEKMQMNRKISYFLDKNE
                     KLYNWIRLICPDGRQILYKAGTEPFYEAAEAEHTPAGHATNAQITGKKISEETGWYEM
                     YIEKQVEANGAVYDLVLSMDLEQLYRQIVEPVKIGKGGYSIVKDQDT"
     gene            complement(627..1313)
                     /locus_tag="ACZPCL_01520"
     CDS             complement(627..1313)
                     /locus_tag="ACZPCL_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1161203.1"
                     /translation="MNTEEILMVDDERAIRLAVRTALTREGMHVTEAADGSTALDLLK
                     QKHFDLVILDVMMENVSGYQVLQTMRAWGDHTPVMMLSGKSDEMDQVLGLGFGADSYL
                     TKPFHTSVLIQTAKALIRRNQIYSQASEARIHKGPFAVDTLKMECLKNGVSLNFTARE
                     QALFRFLLEHPGQVFTKAQLYSQVWNEAVVDDNTITVYIKRIRSKIEENPKEPKYLKT
                     IRGIGYMLEV"
     gene            complement(1473..2774)
                     /locus_tag="ACZPCL_01525"
     CDS             complement(1473..2774)
                     /locus_tag="ACZPCL_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0046942 - carboxylic acid transport
                     [Evidence IEA];
                     GO_process: GO:1905039 - carboxylic acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HIR1161204.1"
                     /translation="MAAAIGFLMIIVIVALLLKGKMSPIVVLAIVPTISALILGFVPT
                     DILGFIADGIKTTTSNGILFIFSVIYFGVLSDTGLFDVIVNWLVKKAGNNVIAVTVVT
                     AMIATIAHLDGTTAVTVLITIPAMYPVYKRMNIDARILLCITGACMGVMNLLPWGGPT
                     ARAATVLQMDATELWHMLIPLQIVGLVMNSVIAVLLGMYAVKKGAGAGKGVEVETNEK
                     EKAETEALKKSRGYLIFNFLLTVAVIAALSTGVGKSYVIFMLGLCVLLAVNYPDQKIQ
                     DKLVKKHAPAALIISATLFAAGAMVGIFDGTGMLEAMANAIMAVIPAAMGKYIHIIFG
                     ILALPFGLCVGTDAYFYGIMPLVMQVGETYGVASLNTALTMLIGKNLALMVSPLVPAT
                     YLAIGLTGTELKDHMKFSIPPYYVVSLIMLVLGVILGIISL"
     gene            2958..5462
                     /locus_tag="ACZPCL_01530"
     CDS             2958..5462
                     /locus_tag="ACZPCL_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009216407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR1161205.1"
                     /translation="MSENKTFKSNIQKIQIRGARVHNLKNINVDVPLNQIVAIAGVSG
                     SGKSSLALGVLYAEGSRRYLESLSTYTRRRMTQASKASVDEILHVPAALALHQRPGVP
                     GIRSTFGTGTELLNSLRLMYSRLASHCCPNGHYLAPTLAVAAGKELVCPECGIHFFAP
                     SAEELAFNSQGACRTCNGTGTVRTVDLNTLVPDDSLTIDEGAVAPWNTLMWSLMTDVC
                     REMGVRTDVPFKELTEKEKDIVFHGPAEKKHIFYHAKNSNQAGELDFTYFNAVYTVEN
                     ALSKVKDEKGMKRVEKFLKEDACPDCHGSRLNERARAPKLQGITLDKACEMTLSQLIT
                     WVDNVPSSLPAEMVPMAESICDSFQTAAKRLMELGLGYLSLDRAASTLSTGERQRMQL
                     ARAVRNRTTGVLYVLDEPSIGLHPVNIVGLTGVMDDLIADGNSVILVDHDTQILSHAD
                     WLIEMGPEAGAKGGQVIAEGTISDIVENKVSRIGAFLKPGYAKRLRPVIQENDLFEHG
                     AISLSTGAIHTVKPLKVNIPKGRLTVVTGVSGSGKTTLILESLVPGIEAAIAKTPLPS
                     HVLSVDPAGISQVKLIDATPIGINVRSTAATYANVHDELRKVFAKTADAKNMGYKAGD
                     FSYNTGKLRCPVCDGTGQVSLDVQFLPDVDIPCPECRGSRYSKEASLIKYTNKKGQSV
                     SLPELMDMDIHTALEACDDLKLVKQRLTVLKELGLGYLTLGEETPSLSGGEAQRLKLA
                     SEMGKLQNDSLFIFDEPTIGLHPLDVQTLLKVFQTLIENEATVVVIEHDLDVIRNADY
                     IIDMGPGGGADGGRIVACETPEQLAKDPNSQTGRYL"
     gene            5670..6692
                     /gene="aroF"
                     /locus_tag="ACZPCL_01535"
     CDS             5670..6692
                     /gene="aroF"
                     /locus_tag="ACZPCL_01535"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR1161206.1"
                     /translation="MIIIMKPQASKEAVEKVTSLIESKGLQAHLSEGSQVTIVGVVGD
                     KSRLAGSNIEMSEGVDKVVAVTESYKLVNKKFHPDDSIIPVGNTHIGPGTMTVMAGPC
                     AIESKEQLLSTAFAVKKAGATFLRGGAYKPRTSPYSFQGLEEEGLKYMKEAREATGLN
                     VICEVTSAHAIEAAVKYVDMLQIGARNMQNFELLKEAGKSGMPVLLKRGLCATIDEWL
                     NAAEYIISEGNPNIVLCERGIRTYETSTRNTLDISAIPVIRQKSHLPVIVDPSHATGV
                     RAYVEPLAKAAVAVGADGLMIEVHPDPAKALSDGPQSLTFDAFDKLMADLKPYVDLAG
                     RTFEAR"
     gene            6975..8126
                     /locus_tag="ACZPCL_01540"
     CDS             6975..8126
                     /locus_tag="ACZPCL_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0033730 - arogenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - L-tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HIR1161207.1"
                     /translation="MEKFTMNDTTIAFIGLGLIGGSLAKGIKRARPDIRIMAYMRTRS
                     KLEQAKKDGIVDVILDGIDEQLKECDLIFLCTPVEYNAQYLSKIRSYLKPGALITDVG
                     STKTDIHEEILRQGLTDCFVGGHPMAGSEKTGYENATDHLLENAYYIVTPPEGLDQSG
                     SETGNIPPKYAENANRIIAVAQTVGAIPLVLDYREHDKVVAAISHLPHLVASSLVNLV
                     KDADSPAGTMKRVAAGGFKDITRIASSSPEMWEQICMTNVEPIADMLERYIGSLQDIL
                     SEIKGHQGDAIYKLFEKSRDYRNTITDRAKGSVEPSYAFSVDVADEVGAISTIAVILA
                     AKGISIKNIGINNNRERGEGALKIEFYDETSMTAAWKRLEYYKYEMVRL"
     gene            8152..9429
                     /gene="aroA"
                     /locus_tag="ACZPCL_01545"
     CDS             8152..9429
                     /gene="aroA"
                     /locus_tag="ACZPCL_01545"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR1161208.1"
                     /translation="MEFKKSGPLKGEITVPGDKSISHRAVMFGSLAKGTTEITGFLQG
                     ADCLSTISCFERMGVAIENRGDIVLVHGNGLHGLKKPETILDCGNSGTTTRLISGILS
                     AQNFDVTLTGDASIQKRPMKRIMEPLSLMGADIVSVNGNGCAPLAIHGKALHGIHYTS
                     KVASAQVKSSILLAGLYAEGPTSVTEPYVSRNHSEIMLNLFGAHVTTKDTTATIEPAS
                     ELHSVKVNVPGDISSAAYFIAAGLMVPGSEILIKNVGINPTRDGILHVCRAMGADLTL
                     LNVKEDEGEPVADLLVRASSLHGTEIGGSIIPTLIDELPMIAAMACFAEGTTVIRDAA
                     ELKVKESNRIAVMVENLSAMGADVTETEDGMIIRGGRPLHGAVIESHLDHRIAMTFAV
                     TALCAEGIIQINGAECVNISYPQFYQDLENLFS"
     gene            complement(9518..10039)
                     /locus_tag="ACZPCL_01550"
     CDS             complement(9518..10039)
                     /locus_tag="ACZPCL_01550"
                     /inference="COORDINATES: protein motif:HMM:NF025559.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HIR1161209.1"
                     /translation="MLFRQQEELLDTLIRNTENKTKDQMKTLTQTILPRIALYKVLNQ
                     SGLSQEDVYRHMRNYMLDIVGTEKHLSMVKMERVPGFYFLYSNIFLRVVRRTNLWDSE
                     QAHGKDYFDVTMKKCLWHTACLENGCEKLCPLFCDVDNVTYGGLKKLDFSRTKTLGYG
                     GDCCDFHFFRKRS"
     gene            complement(10008..10157)
                     /locus_tag="ACZPCL_01555"
     CDS             complement(10008..10157)
                     /locus_tag="ACZPCL_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161210.1"
                     /translation="MDLEITENDKRKRHKKDENKTAKADQCIFSQPVWEQQRRYAVSS
                     TGGTA"
     gene            complement(10158..10691)
                     /locus_tag="ACZPCL_01560"
     CDS             complement(10158..10691)
                     /locus_tag="ACZPCL_01560"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR1161211.1"
                     /translation="MIYTCTYPSPLGNILLAADEVGLTGLWFERQKYFANTLPKETIS
                     QETPVLIQAKKWLDVYFSGKEPDFTPALHPAGSPFRQAVWQILLQIPHGQTMTYGEIA
                     KKMAQMQDAPHMSAQAVGGAVGHNEVSIIIPCHRVVGTNGSLTGYAGGIEKKIALLEL
                     EHTDMSGFFIPKKGTAL"
     gene            complement(10964..12448)
                     /locus_tag="ACZPCL_01565"
     CDS             complement(10964..12448)
                     /locus_tag="ACZPCL_01565"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR1161212.1"
                     /translation="MEKAKLQERYQLFIGGQWKDASAHETFKTICPADGSVLAECAQA
                     TKEDVDEAVKAAWEAFKTWKHTTVAERAAVLNKIAAIIDENKEHLAMVESMDNGKPIR
                     ETLAVDIPMAADHFRYFAGCIQAEEGSANILGKDTLSLILREPIGVVGQIVPWNFPFL
                     MAAWKLAPVLASGCCTVFKTSSTTPLSVLEFARLVQDVIPAGVFNVITGSGSKSGQYM
                     LDHPGFRKLAFTGSTEVGRNVALAAAQKLIPATLELGGKSANIFFEDCDWEMAMDGLQ
                     MGILFNQGQVCCAGSRVFVQESIYNRFVEEAVKRFNKVKVGLPWEEETQMGSQIDRRQ
                     MEKILKYVEIGKEEGAKVLCGGVQAKEGELEKGCFLRPTLLGDVTNDMRVAQEEIFGP
                     VACIIKFKTEEEVLDMANDSAYGLGGAVWTRDINRALRVARGVETGRMWVNTYNAIPE
                     GAPFGGCKASGIGRETHKVILEHYTQIKNIMINMAEAPTGFYPA"
     gene            complement(12548..13699)
                     /locus_tag="ACZPCL_01570"
     CDS             complement(12548..13699)
                     /locus_tag="ACZPCL_01570"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1161213.1"
                     /translation="MGTTYYFLPTRNLFGEDSVKETGSLMQSLGGKKTLIVTDAFLAK
                     QGMARMVQDILKDSGVESVVFGGVEPNPKDVNVEAGLKVFKENGCDSIISLGGGSSHD
                     CAKGIGIVASNGGNIRDYEGVDKSQKPMCPMVAINTTAGTASEITRFCIITDTSRHVK
                     MAIVDWRVTPQIAINDPKLMVGMPPSLTAATGMDALTHAIEAYVSTNANPLTDAAATM
                     AITMITRYLPKAVANGEYMKARDKMAYGQYLAGIAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAILLPHVEEFNLIGNANRFRDIAKAMGENIDGLSTMEAARKAIAAIRQLSEQV
                     GIPQNLRSLGVKEEDYEIMAENAMKDVCQLTNPRKATKEQVIGIFKAAY"
     gene            complement(13862..15259)
                     /gene="pdxR"
                     /locus_tag="ACZPCL_01575"
     CDS             complement(13862..15259)
                     /gene="pdxR"
                     /locus_tag="ACZPCL_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070279 - vitamin B6 binding [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:2000142 - regulation of DNA-templated
                     transcription initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MocR-like pyridoxine biosynthesis transcription
                     factor PdxR"
                     /protein_id="HIR1161214.1"
                     /translation="MLHYELEKKKGSSLYEELYQKLKEDILRGRILPGTKLPSKRELA
                     RDNGISVKTVLSAYEQLVVEGYLYSKEKKGYFVAAVEAMPEYKPVVAEYPQLYREDQW
                     FADFTANNTVYDKFPFSMWRKVMREVLSEYDKELVKRGHFLGVRQLRETIADYLYRTR
                     GMTISPECIVIGASIEYLYSRLIKLLPADVVYAVENPGYKKIPVIYEEYGLKWKSIAM
                     DDGGIHMKSLQDSGADIIHVSPEHHYPLGTVMSASRRQELLSWASEKEERYIIEDDYD
                     CEFRYRSRSIPALGSMDSHESVIYMNTFSKTLTPAIRISYMVLPEKLMRRYIDTANFY
                     CNTASTCEQYALARFIENGYFERHLSRLKKFYHAEGERLKRILKQSVLLPAVSVDGGE
                     SGTHLLVKVDTSMTDTEIEEAAENMGIHISCLSRFCSEKNPEYDHILVLNYSDMDESR
                     LTEAVRRLGNIFIQW"
     gene            15359..16150
                     /locus_tag="ACZPCL_01580"
     CDS             15359..16150
                     /locus_tag="ACZPCL_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1161215.1"
                     /translation="MGVYAITGGSGGIGLRVISQLEKQGHETINIDWKTGDIQADLSI
                     PEGRQKVIDELHELHPEGLDGLILCAGVPGSCHDLRLILSLNFFGTISIIKGAYDLLE
                     KKGGSCVATVSNAISQGDLRMDLADILNNNNEDELRILDLVSNLDENDLLTGNRLYVA
                     SKYALARWVRRHSASYAANGVRINAVAPGNVNTSMTATMSVDEKTALNALPIPTKYGK
                     ETLMEPDEIASAINFLISKEARGVNGIIMFVDGGTDALLNSEKVY"
     gene            16187..16522
                     /locus_tag="ACZPCL_01585"
     CDS             16187..16522
                     /locus_tag="ACZPCL_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2-like protein"
                     /protein_id="HIR1161216.1"
                     /translation="MEPVIQKYLTALETKDFEALAELFTKNGHYCDYCANGTSQHEFH
                     LYGKEAISMFFRNKFLFCQYSILSPTILNGSQAEFIASYGGYQVMAIASLQQLTADGH
                     IRRLTVRPK"
     gene            16565..17155
                     /locus_tag="ACZPCL_01590"
     CDS             16565..17155
                     /locus_tag="ACZPCL_01590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1161217.1"
                     /translation="MIKEIESRRSIRRYKDIPVKEELIEEILKAGMLAPSSKNRQPWH
                     FIVTTGSAKEQVLKAMEAGLKREEKQPFLPESAPYLDGARATLKIMKKAPVIIFITDP
                     LASPMDQPLAVDERVSEICNAQSVGAAIENMTLAATELGLGSLWICNTFFAQEELNHW
                     LNADGQLYAALAVGYADEAPAPRPRRKAELTVEWRK"
     gene            complement(17167..17940)
                     /locus_tag="ACZPCL_01595"
     CDS             complement(17167..17940)
                     /locus_tag="ACZPCL_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR1161218.1"
                     /translation="MDILTGILIPFIGTTAGSACVFFLRGELKPGVQKAFLGFASGVM
                     VAASVWSLLIPAMEMSDGLGRLAFLPAVTGFLLGMAFLLFLDMVVPHLHMDTDQPEGA
                     KSNWKKSTMLVLAVTLHNIPEGMAVGVAFAGMLAKSESITLAGAMALSLGIAIQNFPE
                     GAVISLPLKEAAGQKKAFVYGMLSGIVEPIGAGLMLLLAEKLEPFMPYFLSFAAGAML
                     YVVVEELVPEASEGEHSNIGTIGFAIGFALMMVLDVALG"
     gene            complement(18017..18655)
                     /locus_tag="ACZPCL_01600"
     CDS             complement(18017..18655)
                     /locus_tag="ACZPCL_01600"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1161219.1"
                     /translation="MSDKHYVITIERQYGSGGRITGMRLAEELGIHYYDEEILKMTSE
                     TSAIGEQYFRLADEKAGNNLLYRTVTNMKPELSAPVKDGHRLTSPENLFRFQSEVIRK
                     LAESENCIIIGRCGNYVLQDQLDDVVRIFVYADTVTRVRRVMDVDKVDEEEALRRMKK
                     IDKERTEYHKYFTGRNWMDMENYDLPINASRIDYDQMIKLIKDYLKLRGMID"
     misc_binding    18838..19086
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     assembly_gap    19009..19018
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19161..20252
                     /locus_tag="ACZPCL_01605"
     CDS             19161..20252
                     /locus_tag="ACZPCL_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1161220.1"
                     /translation="MKKSMAFLLTAAMGAALLTGCGSSSSTATTAADTTAAPSSEAAS
                     SEEASSEAENTADADGSYTIGIGQFAEHGSLDNCREGFLEGLAAEGIEEGKNLTVLYQ
                     NAQADGGTSAQIANTFAGKNVDLMCGIATPMAQAEYSVAMKSDIPVIFTAVTDPVAAE
                     LANADGTPIGEITGTSDKLPVEQQLKMIREILPDAKTIGIMYTTSEVNSESAIAEYKE
                     LAPNYDFEIVDSGISSSADIPLAADTLIGKVDCITNLTDNTVVASLPVILSKASAKNI
                     PVFGSEIEQVKIGCLAAMGLDYVELGKQTGEMAAKVLKGEAKASELNFETIKDAAFYG
                     NTKVAENLGITLPESLTGNAAELFDSITE"
     gene            20286..21227
                     /locus_tag="ACZPCL_01610"
     CDS             20286..21227
                     /locus_tag="ACZPCL_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161221.1"
                     /translation="MTTIIFGILEEGLVYAIMALGVYITYKILDFPDLSVDGTFPLGA
                     AVTAAGIANGLPFIGTISPVAALFISFTVGALAGCITGLIHVKLKVRDLLSGIIVMTA
                     LYSINLRIAGKANLPIFSKETIFSNSFLSAHVPEAASPFIVTIILFVIVMICKVLLDA
                     YLQTRSGYLLRAVGDNDVLVTSLAKDKGLVKIVGLAIANGFAALAGSVYCQQKGFFEI
                     SIGTGTMVIGLANVIIGTQLLKRVGFIRSTTAVIIGSIVYKACVSIALLLNDLHIAGL
                     DLSIPVTASDLKLITSVLFLIILVVSPSGGKKVKSHA"
     gene            21220..21993
                     /locus_tag="ACZPCL_01615"
     CDS             21220..21993
                     /locus_tag="ACZPCL_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1161222.1"
                     /translation="MLELKHIHKYYNAGTVNEMCLFNDFNLTIEDHQFVSVVGSNGSG
                     KTSLLNIICGSIPLDDGRIIIGGEDITKMPEYKRQRRIGRVYQNPSMGTCPSMTIAEN
                     MALADNKGKAFNLRPGTNKQRLEFYRESLRSLGLGLEDKMDVKVGVLSGGQRQAMALL
                     MSTMTPIEFLILDEHTAALDPKTAETIMELTNKVVREKNLTTIMVTHNLRFAVEYGDR
                     LLMMHQGHAVIDKAGAEKDATTIDHILKKFNEISIECGN"
     gene            complement(22046..22780)
                     /locus_tag="ACZPCL_01620"
     CDS             complement(22046..22780)
                     /locus_tag="ACZPCL_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161223.1"
                     /translation="MLRKYHLYAAVLCSAAVLGLGGCSNKVDAATTLPLVNESRPVVV
                     ESEEPTSSAEETTVVETVSGTIESAEEQPEQAEDSVCLFGPATQMEDGRLSIDSQADQ
                     GYQGEVILNVSQESTYVLDAVSGLPIELSDIKDGDTIYAYIGPAMTMSLPPMTNATMI
                     FANVPADFKVPDYVTVKSVVTDAASSQSVLTAMDGTEYTLADDCGIVPYLTRNIVTLD
                     DLTQGRKAVVWSDGENTATRIMVFAE"
     gene            complement(23095..24129)
                     /locus_tag="ACZPCL_01625"
     CDS             complement(23095..24129)
                     /locus_tag="ACZPCL_01625"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HIR1161224.1"
                     /translation="MGEIKGRTFDLLTLGQLLLRLSPPDNDRLSRGDTFVKQVGGAEL
                     NVAVGASLLGLHTGVISKLPSHDIGSFMKGKIRSFGVSDDFFMYDHSPSARVGIYYYE
                     NGAYPRKPKVIYDRMNSSFFTLDIDEIPEEVYTASKCFHTTGITLALSEKIRETTIEM
                     IKRFKEAGTLVSFDVNFRGNLWTGDEARECIERILPYVDIFFCSEDTARLTFLKTGTA
                     KEMMKSFTEEYPISIVASTQRIVHSPKRHTFGSVIYDAARDTFYEEEPYRDIEVVDRI
                     GSGDAYISGALYGLLSSGGDCQKAVAFGNATAAVKNTIPGDLPSSNLEEIETIIKAHN
                     QTGPQSEMAR"
     gene            complement(24278..25309)
                     /locus_tag="ACZPCL_01630"
     CDS             complement(24278..25309)
                     /locus_tag="ACZPCL_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1161225.1"
                     /translation="MAKIVTMGEIMLRLSTPNNEKFIQADEFDVNYGGGEANVAVSLA
                     NYGHDVQFVTAVPANPIGECAVAALRKYNVGTKYIARSGERLGIYYLETGSAMRASNV
                     VYDRAHSSISTATADEFNFDEIFADADWFHFTGITPAVSDAAIELTEAALKAAKAHGV
                     TVSVDLNFRKKLWSSEKAQKVMTNLMQYVDVCIGNEEDAEKVLGFKPGNTDVTSGELE
                     LAGYVDIFNQMADKFGFKYIISSLRESHSASNNDWSACIMDGQTREFYHSRKYHIAPI
                     VDRVGGGDSFAGGLICGLADGKDMKAALEFAVAASALKHTIPGDFNLVTRAEVETLAG
                     GDGSGRVQR"
     gene            complement(25389..26039)
                     /locus_tag="ACZPCL_01635"
     CDS             complement(25389..26039)
                     /locus_tag="ACZPCL_01635"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HIR1161226.1"
                     /translation="MKKEHVLARLKEDCLVAVVRAKNYEQGEKVVDAIIEGGINFIEL
                     TMTMDDGDPVGFIKKMADKYKDNDKIVIGAGTVLDPETARACILAGANYIVSPGLNVE
                     TIKLCNRYRVPMLPGVMTPSEAITAMEAGCDVIKIFPGNIVGPAAISSFKGPLPQGEF
                     MPSGGVDVDNVDKWIHAGAYAVGTGSSLTKGAKTGDFAAVTAKAQEFVKAVAAARA"
     gene            complement(26173..26931)
                     /locus_tag="ACZPCL_01640"
     CDS             complement(26173..26931)
                     /locus_tag="ACZPCL_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR1161227.1"
                     /translation="MKLNRTTLRTVDILKMVSKKPDGITLDEICEKLELPKTSAYDII
                     TTLAHTGMVHVVKGQKQRYTIGLTAYRVGINYTNNLDFIGTIDPVLKAFAKEVGKTVF
                     FGVRSDDSIVYICKFEPENPIITTATVGTKNPLYCTSLGKAIMANMEQEERDALADRI
                     NFRPHTQRTIQDKTHFIEELDRVRENGYAFDARELEEHMECVGSPVFGPDGNVIGAIS
                     VSSLYKPTEDYDALGRLVCEKAKALSRLLGFLGK"
     gene            complement(27146..28630)
                     /locus_tag="ACZPCL_01645"
     CDS             complement(27146..28630)
                     /locus_tag="ACZPCL_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR1161228.1"
                     /translation="MQDIVKINQNDNVAVALRPLNEGETLDVAGEKVTLVQDIPQGHK
                     FALRAIAQGEEVVKYGFRIGYAKEAIEKGAWVHVHNVKTALGDVLSYEYKPEGSKDVE
                     PSGHVYFDGYRRADGKVGIRNEIWIVPTVGCVNSIAKALETAAKKFVGGNVEDIIAFQ
                     HPYGCSQMGDDQENTRKVLADMIHHPNAAGVLVLGLGCENSNIPVLMDYIGEYDEQRV
                     KFLQCQDVEDEMETAMGILEELATYANAFSREKIDASELIIGMKCGGSDGLSGITANP
                     VVGAFSDLLVSKGGTTILTEVPEMFGAETLLMNRCATPELFDKTVALINNFKNYFTSH
                     NQTIYENPSPGNKKGGISTLEDKSLGCTQKSGSSLVKGVLEYAEPVKVKGLNLLSAPG
                     NDLVAATALAVSGAHMVLFTTGRGTPFASPVPTVKISSNSKLAGHKSNWIDFNAGQMV
                     EDKTKEELAQDLLNYVLEVASGRKVKSEAAGFHDMAIFKQGVTL"
     gene            28870..29874
                     /locus_tag="ACZPCL_01650"
     CDS             28870..29874
                     /locus_tag="ACZPCL_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1161229.1"
                     /translation="MNIYDVSERAGVSIATVSRVLNGNPNVSERTRKKVLAVMDELGY
                     TPNVFARGLGLDTMQTIGIMCSDSSDPYLAEAIYYLERELRSHNYDSILCCTGYDLDV
                     KQKYFNLLRSKRVDAIILAGSKFVEMRSRDNNYIIEAAADGLPVMLVNGYLEGKNIYS
                     TVCDDYASMYDATLQLIHSGHSRILYLYTSVSYSGVNKMEGYKAALKENRIPVEDSMI
                     HQCSKSFENSRDLLLSLKEEGLKFDAVLASDDSLAVGAVKYAHTAGISMPEELSIIGY
                     NNSLLARCTDPEITSIDSKVEALCTTTVNTLMGVFSGANVPNRTTIASALIKRKTTNF
                     "
     gene            29904..>30483
                     /locus_tag="ACZPCL_01655"
     CDS             29904..>30483
                     /locus_tag="ACZPCL_01655"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HIR1161230.1"
                     /translation="MKQFLDKDFLLSTDMAKTIYHDYAENMPILDYHCHINPQEIYED
                     RKFENITQVWLGGDHYKWRQMRSNGVDEKYITGDGTDREKFQAWAETMPKLIGNPLYH
                     WSHLELRRYFGYEGYLNGDTAEEVWNLCNAKLQEDSMTVRNLIKQSNVTLICTTDDPV
                     DSLEWHKKLAEDTTFDVQVLPAWRPDKAMNVEK"
BASE COUNT         8189 a         7311 c         6352 g         8621 t
ORIGIN      
        1 gtatcctggt ccttaacaat agagtatccg ccctttccga tctttacagg ctctacgatc
       61 tgacggtaca gctgttccag atccatagaa agaacaagat cataaacagc tccatttgct
      121 tcaacctgct tttctatgta catttcatac cagcctgttt cttctgatat cttttttccc
      181 gtgatctgtg cattcgtggc atgtcccgcc ggagtatgtt ctgcctcagc agcttcgtaa
      241 aaaggctctg tacctgcttt ataaaggatc tgcctgccat caggacatat cagacggatc
      301 cagttataca atttctcatt tttatccaga aaataggata ttttccggtt catctgcatt
      361 ttttccccta aaagcccatt ttctgatgag taaaaaaggt cgatctgctc cagctgttca
      421 ttaaggaaca tgtcaagatt atttcctatg atcccggcta tggtaagcag ctggctttcc
      481 tctgtatgga ttacctgctc tttataacgc ttataaaatt ctgcacagga taatgccata
      541 accagtgaca gaaacactac cagaaaaaca gcgcctattg ctgcctgtct ttttcctttt
      601 gtttccttga tccatttcat tttttatcag acctccagca tatatccgat tccccgtatt
      661 gtttttaaat atttaggttc ttttggattt tcctcgatct tgcttctgat ccgcttaata
      721 taaaccgtga tcgtattatc atctaccact gcctcattcc acacctggct gtaaagctgt
      781 gcctttgtga atacctgtcc cggatgctct aacagaaagc ggaatagcgc ctgttccctt
      841 gctgtaaaat tcagggagac tccgtttttt aaacactcca ttttcaaagt gtccacagca
      901 aagggacctt tatggatcct tgcttccgat gcctgactgt atatctggtt tctccggatc
      961 aatgcttttg ctgtctgtat cagcacagaa gtatgaaatg gttttgtcag ataactgtct
     1021 gccccgaatc ccagccccag cacctggtcc atctcatctg actttccaga aagcatcatc
     1081 accggtgtat gatctcccca agcccgcata gtctgtaata cctgatagcc gctgacattt
     1141 tccatcatca catccaggat caccagatcg aaatgtttct gttttagtaa atctaacgct
     1201 gtactgccat cagctgcttc tgttacatgc attccttccc tggtcaaagc cgtccgtaca
     1261 gccagacgga ttgctctttc atcatctacc atcaatattt cttccgtatt catgttcatt
     1321 tccccattct attttttgct ctttcaagcg catttgtacc tataaaagag tatagcaaat
     1381 atataaaaat aagaccataa ccggctggct ttttctcaca gccggttatg gtcttttgtg
     1441 ttcttactat gcttttatgg tcttactgtc tcttataaac ttatgatccc taaaattact
     1501 cccagtacta acatgatcag gcttaccaca taatagggcg gaatgctgaa tttcatatga
     1561 tcctttaatt ctgttcctgt caatccgata gccagataag tagccgggac aaggggactt
     1621 accattagcg ccagattctt tccgatcagc atggtaagtg ctgtgttcag ggatgcaacc
     1681 ccgtatgttt cgccaacctg catgaccaga ggcatgatgc cgtaaaaata tgcatcggtt
     1741 cccacacaaa ggccaaaagg aagggcaagg ataccaaaaa taatgtggat gtattttccc
     1801 atagctgcag gaatgaccgc catgatcgca tttgccattg cttccagcat tcctgttcca
     1861 tcaaaaatac ctaccatagc gcctgccgca aacaaggttg ctgaaatgat cagtgctgcc
     1921 ggagcatgct tctttaccag tttatcctgg atcttctgat ccgggtagtt tactgccagt
     1981 aaaacacaaa gtcctaacat aaagatcaca taggattttc ctacaccagt tgataatgct
     2041 gcgatcacag caaccgtaag aaggaaatta aagatcagat atcctctgga ttttttcagt
     2101 gcttctgttt ctgccttttc tttttcattt gtctcaactt ctacaccttt tcctgcaccg
     2161 gcaccctttt ttaccgcgta cattccaagc aaaacggcga tcacactatt catgaccaga
     2221 ccaacaatct gaagcgggat cagcatatgc cacaactctg ttgcgtccat ctgaagtaca
     2281 gttgccgccc ttgctgtagg tccgccccat ggaagcagat tcatgacacc catacaggct
     2341 ccggtaatac acagtaagat ccgggcatca atattcattc tcttgtatac tggatacatt
     2401 gccgggatcg taatcagaac tgttacagcg gtggttccat ccaaatgggc aatggttgcg
     2461 atcattgcag ttaccacagt aactgcgatc acattatttc cagccttttt tacaagccag
     2521 tttacgatca cgtcaaacaa tcctgtatca gacaaaaccc caaaataaat aacagaaaaa
     2581 ataaacaaaa taccattact ggtagttgtc ttaatgccat ctgcaataaa tcccagaatg
     2641 tctgtaggaa caaaccccag gatcagggct gagatggttg ggacgatagc caatactaca
     2701 ataggtgaca ttttcccctt taaaagaagg gctacgatca cgatgatcat taaaaatcca
     2761 attgctgctg ccataatttt ttcctccttc atttgtattt tattttatac atacaaaatt
     2821 aaatgatcat gactctaaaa tgacagtttt gtcataattg aattacggtt ccgtcatgtg
     2881 acggtctgct tttcttgcaa ttccttacgg agattgttaa aatagttgca gaataatgag
     2941 aaaggtatgc acattttatg tctgaaaata aaacctttaa aagcaatata cagaaaatac
     3001 agatccgcgg cgcccgggtg cataatctta aaaatatcaa tgtagacgta cccttaaacc
     3061 agatcgtagc cattgccggt gtctccggct ccggaaagtc ctctctggct ttaggcgtac
     3121 tatatgcaga gggctccaga cgctatctgg agtctctttc tacctataca agaaggcgca
     3181 tgacccaggc atccaaagcc agtgtggatg agatcttaca tgttcccgct gctctcgccc
     3241 tgcatcagcg tcccggcgtt cccggcatcc gaagcacctt tggaaccggt acagaacttt
     3301 taaacagcct gcgcctgatg tattcccgtc tggcttccca ctgctgtcca aatggacatt
     3361 atctggctcc cacactggca gtggctgccg gaaaagaact ggtctgccct gaatgcggca
     3421 tccacttttt tgctccttct gcagaggaac tggcatttaa cagccaggga gcctgccgca
     3481 cctgcaatgg aacgggaacg gttcgcaccg tagacttaaa taccctggtg ccagatgact
     3541 ccttaaccat tgacgaaggg gctgtggctc cctggaatac tcttatgtgg tctttgatga
     3601 ccgatgtatg ccgggaaatg ggtgtgcgca ccgatgtgcc ttttaaggaa ttaacagaaa
     3661 aggaaaaaga catcgttttc catggacctg ctgagaaaaa acatattttc tatcacgcca
     3721 aaaactccaa tcaggcagga gaactggatt ttacctactt taacgctgtc tacaccgtag
     3781 aaaatgctct ttccaaagta aaagatgaaa aaggtatgaa gcgggtggag aagtttttaa
     3841 aagaagatgc atgcccggac tgccatggtt cccgtttaaa tgaacgggcc agagcgccga
     3901 aattacaggg gatcactctt gataaagcct gtgaaatgac cttatcccag ctgatcacat
     3961 gggtggacaa tgttcccagt tctctcccag cggaaatggt gccaatggca gaaagcatct
     4021 gcgactcctt ccagacagct gcaaaacggc ttatggagct gggacttggc tatctctcct
     4081 tagaccgggc tgcttctact ctttctacag gggagcgcca gagaatgcag ttagccagag
     4141 ccgtgagaaa ccgaaccaca ggtgtcctct atgtactgga cgagccgtcc attggactcc
     4201 acccggtaaa tatcgttgga ctgacagggg ttatggatga tctgatcgct gacggaaatt
     4261 ccgttatttt agtagaccat gacacacaga tcttatccca tgcagactgg ctcattgaaa
     4321 tggggccgga agcaggcgca aaagggggac aggtgatcgc agaagggact atttctgata
     4381 ttgtggaaaa taaagtatcc cgcatcggtg cttttctaaa gccgggatac gccaaacgcc
     4441 tgcgtccggt gatccaggaa aatgacctgt ttgaacatgg ggccatctcc ctttctaccg
     4501 gtgccatcca tacagtaaaa ccacttaagg taaatatccc caaaggccgt cttaccgttg
     4561 taaccggtgt ttccggttct ggaaagacca ctcttattct ggaaagcctt gttcccggca
     4621 tagaggcagc tattgcaaaa acgcctctgc cttcccatgt cctttctgtg gacccggcag
     4681 gtatttccca ggtaaaactg atcgatgcaa ctcctattgg catcaatgtc cgctccactg
     4741 ctgctactta tgccaatgtc cacgatgaac tgcgaaaagt atttgcaaaa acggcagatg
     4801 ccaaaaacat gggatataaa gctggtgatt tttcctataa tacaggaaag cttcgctgcc
     4861 ccgtatgtga cggcaccggc caggtcagcc tggacgtaca gtttctgccc gatgtggaca
     4921 ttccatgccc ggagtgcaga ggttccagat attccaaaga ggcatctctt attaaataca
     4981 cgaataaaaa agggcaaagt gtttctctcc cggaacttat ggacatggac atccatacag
     5041 cccttgaagc ctgcgatgac ctcaaactgg taaaacagcg gctcactgtg ttaaaagagc
     5101 ttggacttgg atacctgact ttaggagaag aaacacccag tctttccggc ggtgaggccc
     5161 agagactgaa attggcaagc gaaatgggca agctgcaaaa tgactccctg tttatctttg
     5221 atgaacctac cattgggctg catcctctgg atgtgcagac tctccttaag gtatttcaga
     5281 ccctgatcga aaatgaagca actgttgtgg tcattgaaca tgacctggat gtgatccgca
     5341 atgctgacta tattattgat atgggacctg gaggcggagc agacggcggc cgtatcgttg
     5401 cctgcgagac accggaacag cttgcaaaag atccaaacag ccagaccggc cgctatttat
     5461 agcatgagtt acataaaagc gtaaaagttt aaagcattaa agtgttgact tttacgcttt
     5521 tatgtattaa actgtctgca agttaagaga tacctggatt gaaaaagcac atcatgctca
     5581 ccgcaatccg atatctggta aaaaggggca tctaacatac agagccgata tacaagaaac
     5641 cattatgcgg ataaggagta taacagacta tgatcattat tatgaaacca caggcttcca
     5701 aagaagctgt tgaaaaagta acttccctca ttgagtcaaa aggacttcag gcccatcttt
     5761 ctgaaggttc ccaggtcacg atcgttggtg ttgtaggtga taagagcaga cttgccggaa
     5821 gcaatattga aatgagcgaa ggtgttgaca aggtcgttgc tgtcacagag tcttacaaac
     5881 tggtcaacaa aaaattccac ccggatgact ccattattcc tgtaggcaac acccacatcg
     5941 gaccaggaac catgactgtt atggcaggtc cctgcgctat tgagtcaaag gaacagctgc
     6001 tttccactgc tttcgctgta aagaaagctg gggctacctt ccttcgcggc ggtgcttaca
     6061 agccccgtac ttccccatac tctttccagg gactggaaga agaaggctta aaatatatga
     6121 aagaagccag agaagcaacc ggactgaacg tgatctgtga agttaccagc gcccatgcca
     6181 ttgaagctgc tgttaaatat gtagacatgc tccagatcgg cgcaagaaat atgcagaact
     6241 tcgagctgtt aaaagaagcc ggaaaatcag gaatgcctgt attgttaaaa cgcggcttat
     6301 gtgctaccat agatgagtgg ctgaatgcag ctgaatacat tatttcagaa ggaaacccaa
     6361 acatcgtttt atgtgagcgt ggtatccgca cctacgaaac ttctaccaga aacaccctgg
     6421 atatcagtgc gattcctgtg atccgccaga agagtcatct tccagtgatc gtagatccaa
     6481 gccatgcaac cggcgtaaga gcctatgtag aacctcttgc aaaggctgca gttgcagtag
     6541 gtgccgacgg actgatgatc gaggttcatc cagatccggc aaaagccctt tctgacggtc
     6601 ctcagtctct tacctttgat gcatttgaca aattaatggc tgacttaaag ccatatgtgg
     6661 atctggctgg cagaactttt gaagccagat aaccagatag tcacctatca ttcacagccc
     6721 aaacacctga aaacctttat gttctctcgg aatctttctg tgcttgattt tgtgagaaga
     6781 ttttttgtca gttgtgccca aatttctgag gcgtacgcgg tgcgtacgtt gatgaaattc
     6841 gggtgctact ggcggaaaat cggccacaaa agcaggtgca ggaaagactc cgagaggaca
     6901 tctatctttg tgaaggtttt caggtgattc tgtatctccg atagaacatt ctatatgtaa
     6961 ggagcgattt taaaatggaa aaatttacca tgaatgatac aaccattgcc tttatcggcc
     7021 ttggccttat cggcggttcc ttagcaaagg gcattaaaag agcccgtccg gatatccgca
     7081 tcatggccta tatgcgcacc cgttccaagc tggagcaggc aaaaaaagac ggcattgtag
     7141 atgttatttt agacggcatt gacgaacagt taaaagaatg tgacctgatc tttttatgta
     7201 caccggtgga atacaatgca cagtatctgt ctaagatccg ctcctattta aaaccgggag
     7261 ctttgatcac ggatgtagga agcaccaaaa cggacatcca cgaagagatc ctgcgccagg
     7321 gccttacaga ctgctttgta ggcggtcatc ctatggccgg ttctgaaaaa acaggatacg
     7381 aaaacgcaac ggatcatctc cttgaaaatg cttattatat tgttacaccg ccggaaggcc
     7441 ttgaccagag cggcagcgaa accggtaata ttccaccaaa atatgccgaa aatgccaacc
     7501 gtattatcgc agtggcccag accgtaggtg ctatcccact ggtacttgac tacagggaac
     7561 atgataaagt ggtggctgcc atcagtcatc tgccccacct ggttgcttcc agccttgtaa
     7621 atctggtaaa agatgccgac tcacctgccg gaaccatgaa acgggtagct gcaggcggtt
     7681 ttaaggatat tacccgtatt gcgtcttcct ctcctgaaat gtgggagcag atctgtatga
     7741 ccaatgtgga acctattgca gatatgttag aacgttatat cggttccctt caggacatcc
     7801 tttctgaaat aaaaggacat cagggagacg cgatttataa actgtttgaa aaatccagag
     7861 actaccgaaa taccattacc gaccgggcca agggttctgt ggaaccgtct tatgcctttt
     7921 ctgtagatgt ggccgatgag gtaggtgcga tctccaccat cgcagttatt ctggcagcca
     7981 aaggcatcag tattaaaaat attggtatca ataacaacag agaacggggc gaaggtgcct
     8041 taaagatcga attttatgat gaaacatcta tgaccgcagc ctggaaacgg ctggaatatt
     8101 ataaatacga aatggtaaga ctgtaacgtc tacctgtctg ggaggaaaca tatggaattt
     8161 aaaaaatcag gcccattaaa gggagaaatc acggttccgg gagataaatc tatttcccac
     8221 cgtgccgtga tgttcggctc tcttgcaaag ggaaccaccg agatcacagg atttttacag
     8281 ggagctgact gtctgtctac catctcctgt tttgaacgta tgggcgttgc cattgaaaat
     8341 agaggggata tcgtcctggt acatggaaat ggccttcacg gtcttaaaaa gccggagacc
     8401 atactggact gcggaaacag cggcactact acccgcctga tctccggtat tttatctgca
     8461 cagaactttg acgtaacttt aacaggtgac gcttccatcc agaaacgtcc catgaagcgt
     8521 attatggaac cactgtccct tatgggcgca gatattgtca gtgtaaatgg aaatggctgc
     8581 gcgcctctgg cgatccacgg aaaagccctt cacggcatcc actacacctc caaagtagcc
     8641 tctgcccagg ttaaatcttc tattttatta gcaggacttt acgcagaggg acctacctct
     8701 gttacagagc cctatgtatc cagaaatcac tctgaaatca tgttaaatct gttcggcgcc
     8761 catgtgacca caaaagatac cactgctact attgaaccgg ccagtgaact gcacagtgta
     8821 aaggtaaatg taccaggaga catttcttct gctgcctact ttattgcagc aggacttatg
     8881 gtacccggct ctgagatcct gatcaaaaat gtaggcatca atcctaccag ggacggcatc
     8941 cttcatgtat gcagggctat gggtgcagac cttactttat taaatgtaaa agaagacgaa
     9001 ggggaaccgg ttgctgatct tttagtccgc gcttcttccc ttcatggaac agagatcggc
     9061 ggcagcatta tcccgactct tattgacgaa ctgcctatga tcgctgctat ggcctgcttt
     9121 gcagagggaa cgaccgtgat ccgggatgca gcagaattaa aggtaaaaga gtccaaccga
     9181 attgctgtta tggtggaaaa tttaagtgct atgggagctg acgtaacaga aacagaggac
     9241 ggcatgatca tccgcggcgg cagacctctt cacggtgctg tgatcgaaag ccacttagac
     9301 caccgcattg ccatgacctt tgcagtgaca gctctctgcg cagagggcat tatacagatc
     9361 aatggggcag aatgtgtgaa tatttcttat ccacagtttt accaagatct tgagaacttg
     9421 ttttcataaa tacggaaaca agatgaatag caacaaaagg cgtatgagtc cataccagga
     9481 tccacacgcc ttttatatgc tcatttttca gtcccgatca ggatcttttc ctgaaaaaat
     9541 gaaaatcaca gcagtctccc ccgtaaccta atgttttagt acgggaaaag tccagttttt
     9601 tcagaccacc atatgttaca ttatcaacat cacaaaacag gggacaaagc ttttcacagc
     9661 cattttctaa acaggctgta tgccacaggc attttttcat tgtcacatca aaataatcct
     9721 ttccatgggc ctgctcactg tcccacagat tcgttcttcg cacaacacga aggaaaatat
     9781 tgctgtaaag aaaataaaat cccggcaccc gttccatttt taccatggac aaatgctttt
     9841 ctgttccaac gatatccagc atatagtttc gcatatgcct gtagacatcc tcctgtgaca
     9901 gaccactttg gttcagtacc ttgtacaggg ctatacgggg caaaattgtc tgagtcagcg
     9961 ttttcatctg atcctttgtc ttattttctg tatttctgat cagtgtatca agcagttcct
    10021 cctgttgacg aaacagcata tctcctctgc tgttcccaaa caggttgact aaaaatgcat
    10081 tgatctgcct ttgctgtttt attttcatcc tttttgtgcc tttttctttt atcattttct
    10141 gtgatctcaa gatccattta aagggcagtc cccttctttg gaataaagaa cccgctcata
    10201 tctgtatgtt ccagttctaa cagcgcgatc ttcttttcaa ttcccccggc atatccggta
    10261 agacttccat tggttcctac aacacgatga caggggatga taatggaaac ttcgttatgt
    10321 cctactgctc cgcctacagc ctgtgcagac atatgaggcg catcctgcat ctgtgccatc
    10381 ttttttgcaa tctctccata tgtcatagtc tgtccatgag ggatctgcag taagatctgc
    10441 catacagcct ggcgaaacgg ggaacctgca ggatgaagtg caggagtaaa atccggctct
    10501 ttccctgaaa aatatacatc cagccatttt ttcgcctgga tcagaacggg tgtctcctgt
    10561 gaaatggttt cttttggaag tgtatttgca aagtattttt gtctttcaaa ccataaacca
    10621 gtcagtccaa cctcatctgc cgccagtaaa atatttccca gtggggacgg atatgtacat
    10681 gtataaatca tattaccact tcctttccca tcaactttta catttaagtt tttcgcttac
    10741 acagactctt ttggactcat tatacctcat ttttatctat ccgtctatag ttttatcgaa
    10801 tgtatgttcg ttttgctttt atcttaaact ttccacctga ctctttttgc ataaaaaaac
    10861 gagcagcaaa actggattta cgcttgtttg ctactcgttc aggaattatt atatttctca
    10921 actcaaaaaa tgtcaaaagg atggaatcat tttttattta atcttatgcc ggataaaatc
    10981 cagtaggcgc ttctgccata ttgatcataa tgttcttaat ctgtgtataa tgctccagaa
    11041 tgaccttatg tgtctctctt ccgatgccgg atgctttgca gccgccaaat ggagcacctt
    11101 ccgggatcgc attgtaggtg ttgacccaca tgcggcctgt ttccacgcct cttgccacac
    11161 gaagagcccg gttgatatct ctggtccaaa cagcaccgcc taagccgtag gcactgtcat
    11221 ttgccatgtc aaggacttct tcctctgttt taaacttgat aatgcaggca accggtccaa
    11281 agatctcttc ctgggctacg cgcatatcgt tagtcacatc tcctaataag gtcggacgca
    11341 ggaagcagcc tttttccagt tctccttctt tcgcctgtac accgccgcaa agcacctttg
    11401 cgccttcttc ctttccaatt tccacatatt ttaagatctt ttccatctgt ctgcggtcga
    11461 tctgacttcc catctgggtc tcttcttccc atggaaggcc tacttttact ttattaaagc
    11521 gttttacagc ctcttctaca aaacggttat aaatactttc ctggacaaag actctggaac
    11581 ctgcgcagca aacctggccc tggttaaaaa ggatgcccat ctgaagtccg tccattgcca
    11641 tttcccagtc acagtcttca aagaagatat tggcagattt tcctcccagc tccagtgttg
    11701 caggaatcag tttctgagct gctgccaaag ctacatttcg tcctacttct gtggaaccgg
    11761 taaaagcaag ttttctaaat cccggatgat ctaacatata ctggccggat ttagagccag
    11821 aaccggtgat cacgttaaat acgcctgccg ggatcacatc ctggaccagt cttgcaaatt
    11881 ccagaacact taaaggtgtc gtactggagg tcttaaatac agtacagcag ccgctggcaa
    11941 gaacaggagc cagcttccat gctgccatta aaaatgggaa attccatgga acgatctgac
    12001 ctactacgcc gataggttct cttaagatca ggcttaaggt gtctttgcca agaatattgg
    12061 cacttccctc ttctgcctgg atgcagcctg caaagtagcg gaaatggtca gctgccatag
    12121 ggatatctac tgccagtgtc tcacggatgg gcttgccgtt gtccatggac tctaccatag
    12181 ccagatgttc cttattttca tcaatgatgg cagcgatctt atttaaaacg gcggctcttt
    12241 ctgcaactgt agtatgcttc catgttttaa aagcttccca tgctgctttt actgcctcat
    12301 ctacatcttc ctttgttgcc tgggcacact ctgctaatac gcttccgtct gccgggcaga
    12361 ttgttttaaa tgtttcatgg gcagaagcat ctttccactg acctccgata aataactgat
    12421 aacgttcctg taactttgcc ttttccatat tattttccgc ctttcttatg caatttcttt
    12481 gcagtctttc atgccttttg ccactggacc atttccattt ccattttcat aaagcccgca
    12541 gatcttatta ataagcagcc ttgaaaattc cgattacctg ttctttggta gcctttctcg
    12601 ggttggtcag ctggcatacg tccttcattg cattttcagc catgatctca tagtcttctt
    12661 cttttactcc aagacttctc aggttctgtg ggatacctac ctgctcgctg agctggcgga
    12721 tggctgcaat ggcttttctg gctgcttcca tagtagaaag tccgtcaata ttttcgccca
    12781 ttgcctttgc aatatctctg aagcggtttg catttccgat caggttaaac tcttctacat
    12841 gaggaaggag aatggcgttg cacacaccat gaggcaggtt gtagaaacca cctaactgat
    12901 gggccatcgc atgaacataa cctaaggaag cattgttaaa agcgataccg gccaggtact
    12961 gtccgtatgc cattttgtct cttgccttca tgtattctcc atttgcaact gcctttggca
    13021 gataccgtgt gatcatggta atagccatgg tagcagctgc atctgtcagt ggatttgcat
    13081 tggtagaaac ataagcttca atagcatggg tcaaagcatc cattccggta gctgctgtca
    13141 gtgacggtgg catgcctacc atcagcttgg gatcattgat ggcgatctgt ggtgtcactc
    13201 tccagtctac gatcgccatt tttacatgac ggctggtatc tgtaatgata cagaagcggg
    13261 tgatctcaga agcagttcct gctgtggtgt tgatggcaac catagggcac ataggctttt
    13321 gtgacttgtc cacgccctca taatcccgga tgtttcctcc attggaagct acgatgccga
    13381 taccctttgc acagtcgtgg gaagaaccac cgccaagaga gataatagag tcgcatccat
    13441 tttccttgaa taccttaaga ccggcttcta cgtttacatc cttcgggttt ggctctaccc
    13501 cgccaaatac aacgctttca acaccagagt cctttaagat atcctgaacc atccgggcca
    13561 ttccctgttt tgccagaaat gcatctgtta caatgagagt tttctttcct cccaggctct
    13621 gcattaagga acctgtttcc tttacggaat cttctccaaa taagtttctt gtaggcagaa
    13681 aataataagt tgttcccatg tgtccgtacc tcgtttcttt cttatttttt tcactcattt
    13741 tcctacaggg agtttttatt ttggaacact ttattcctcc ctgtgtgtcg gtaggctcat
    13801 ggtaacactg atctgatatc tttaaaatat ccaaaataaa actattaaag gatatcagat
    13861 tttaccactg gataaaaata ttacccagtc ttctcaccgc ttccgtcagg cggctttcat
    13921 ccatatcaga ataatttaac actaaaatat gatcatattc cgggttcttc tcactgcaga
    13981 accgggacag acaggaaata tggataccca tattttccgc tgcctcttca atctccgtat
    14041 ctgtcatgga agtatctact ttcaccagca ggtgggtgcc gctttctcct ccgtccacgc
    14101 tgactgccgg cagtagcacc gattgtttta agatacgctt taaacgttct ccttctgcat
    14161 gataaaactt cttaagacgg cttaaatggc gctcaaaata gccattttca ataaatcttg
    14221 ccagagcata ctgctcacag gtagaggctg tattgcagta aaaattggcg gtatcaatgt
    14281 agcggcgcat cagcttctcc ggaaggacca tatagctgat acggatcgcc ggtgtcaggg
    14341 tcttgctgaa ggtgttcata tagatcacgc tttcgtggga gtccatgctc ccaagtgcgg
    14401 gaatagaccg ggaacgatag cggaattcac agtcataatc atcctctatg atatagcgtt
    14461 cttctttttc tgaagcccag cttaaaagct cctgacgcct ggatgcagac ataactgttc
    14521 ccaatggata atggtgctcc ggggatacat ggataatgtc tgccccgctg tcttgcaggc
    14581 ttttcatatg gattccccca tcgtccatgg ctatactctt ccacttcagc ccatattctt
    14641 catagatcac agggattttc ttatatcccg ggttttccac agcatagact acgtctgcag
    14701 gcagaagctt gatcagacgg ctatacaggt attcaatact ggctccgatc acaatgcatt
    14761 caggagaaat ggtcattcca cgggtgcggt ataagtaatc cgcaatggtc tcccgcagct
    14821 ggcggactcc cagaaaatga cctcttttta ccagttcttt gtcatactct gacagcacct
    14881 ccctcatgac ctttctccac atggaaaatg gaaatttatc atacactgtg ttgttggctg
    14941 taaaatccgc aaaccattga tcttccctgt aaagttgggg atattctgcc actacaggtt
    15001 tatactccgg catagcttcc actgccgcca caaaatagcc ttttttctct ttggaataaa
    15061 gatagccttc taccaccaac tgttcgtagg cgcttaaaac tgtctttacg ctgatgccat
    15121 tatccctggc cagctccctt ttggaaggca gcttggtccc cggcaggatc cgccctctta
    15181 aaatgtcttc tttcagtttc tgatacagtt cttcatagag agaactgccc tttttcttct
    15241 ctaactcata atgcagcatt tcatctggta tccttttttt tcactattct gggtattaca
    15301 ttggtatcaa tttcgattat aatagaaaga aaattatttg taaaccggag gatcacttat
    15361 gggagtctat gcaattaccg gcggttccgg tgggatcggc ctgcgtgtga tcagtcaatt
    15421 agaaaaacag ggacatgaga ctatcaatat cgactggaaa acaggagata tccaggccga
    15481 cctttctatt ccagaaggac ggcagaaagt aattgatgaa ttacacgagc tgcatccaga
    15541 aggtttagac ggtctgatct tatgcgccgg cgttcccggt tcctgtcacg atctgcgcct
    15601 gatcctttct ttaaactttt tcggaacgat ttctattata aaaggggcct acgatctttt
    15661 agagaaaaaa ggcggcagct gcgtagccac tgtttccaat gctatctccc agggagacct
    15721 gcgcatggac ctggcggata ttttaaataa taataatgaa gatgaacttc gcatcctaga
    15781 tctggtttcc aatttagatg aaaatgacct tcttacaggt aaccgcctgt acgtggcttc
    15841 caagtatgcc ttagccagat gggtccgccg ccattctgcc tcttatgccg caaatggggt
    15901 tcgcatcaac gcagtggcac ctggaaacgt aaacacatcc atgactgcca ctatgtctgt
    15961 ggatgaaaag acggcattaa atgcccttcc tattcctacc aaatatggca aagagactct
    16021 tatggaaccg gatgagatcg cctctgccat taatttcctt atttcaaaag aagcaagagg
    16081 cgtcaacggc attatcatgt ttgtggacgg cggcacagac gcccttctca attcagaaaa
    16141 agtatattaa atatggatca atatttttta tagaaaggaa actttcatgg aacctgttat
    16201 ccagaaatat ctcacagctc ttgaaacaaa agattttgaa gccctggcag aattatttac
    16261 caaaaacggc cactactgtg attactgcgc caatggcaca tcccagcacg aatttcattt
    16321 atatggaaaa gaagccattt ccatgttttt ccggaataag tttctcttct gtcagtattc
    16381 catattatcg cccacgatcc tgaacggctc acaggcagaa tttatcgcct cctacggcgg
    16441 ttaccaggtc atggctattg ccagcttaca gcagctgact gcagacggac atatccgccg
    16501 actgaccgtg cgaccgaaat aatacaatct cacaacatcc tatacaatga aaggatctgt
    16561 actcatgata aaagaaatag aaagcagacg gagcatccgc cgctataaag acattcctgt
    16621 aaaagaagaa ttaatagaag aaatattaaa agccggcatg ctggctcctt cctccaaaaa
    16681 ccggcagcca tggcatttta tcgtaacaac aggctctgca aaggaacagg tccttaaagc
    16741 catggaagca ggactgaaac gggaagaaaa acagcctttt ctcccagaaa gtgcccctta
    16801 tttagatggt gccagggcaa cccttaagat catgaaaaaa gcacctgtta ttatttttat
    16861 cacagaccct ctggcctctc ctatggatca gcctctggct gttgatgaac gtgtgtcaga
    16921 aatatgcaat gcccagtccg tgggagctgc cattgaaaac atgacactgg ctgccacaga
    16981 actgggcctt ggaagtcttt ggatctgtaa tacgtttttt gctcaggaag aattaaatca
    17041 ctggttaaat gctgacggcc agctctatgc tgccttggct gtaggctacg cagatgaagc
    17101 acctgctcca agaccaagac gaaaagcaga actgactgtg gagtggcgaa agtaacacaa
    17161 cctgcattat cccaacgcca catcaaggac catcatcaat gcaaacccaa tggcaaatcc
    17221 gatggtacca atattggaat gttccccttc tgatgcttcc ggcaccagtt cttccactac
    17281 tacgtaaagc atggcacctg ctgcaaagga aaggaaatat ggcataaagg gctccagttt
    17341 ttctgccaat aacagcataa gaccggcacc gatgggctct acaataccgg aaagcatacc
    17401 gtagacaaag gcttttttct gccctgccgc ttcttttaaa ggaagggaga taaccgctcc
    17461 ttccgggaaa ttctggatcg caattcccag tgacaatgcc atagcgcctg ccagtgtaat
    17521 ggactcactt tttgccagca tacctgcaaa agccacacca actgccattc cttccggtat
    17581 gttatgtaag gtcactgcaa gaaccagcat agtgctcttt ttccaattgc tttttgcgcc
    17641 ttccggctga tccgtatcca tatgaagatg aggaactacc atatccagaa acagcaaaaa
    17701 cgccattcct aaaaggaagc cagttactgc cggaagaaaa gccagtcttc caagtccatc
    17761 actcatttcc atagccggga tcagaaggga ccacacagaa gcagccacca tcactcccga
    17821 agcaaagccc agaaatgctt tctgcactcc gggctttaat tctccccgca ggaagaacac
    17881 gcaggcgctg cctgctgtgg ttcctataaa agggatcagt attcctgtta aaatatccat
    17941 ctgctacctc atgttgctac attgctgccc catccatagg cggatctacc atctctaaag
    18001 agcatcgtca taacgatcag tcgatcattc cccgaagttt cagataatct ttgatcagtt
    18061 tgatcatctg atcataatca atacggctgg cattaatagg aaggtcataa ttttccatat
    18121 ccatccagtt tctgccagtg aaatatttat gatactctgt gcgctcctta tcgatcttct
    18181 tcatgcggcg cagtgcttct tcttcgtcta ccttgtctac gtccataact ctgcggactc
    18241 tggtaaccgt atctgcatag acgaaaatgc ggactacatc atctaactga tcctgaagaa
    18301 cataattgcc acagcgtcca atgataatgc agttttcaga ctctgccagc ttgcggatga
    18361 cctcagactg gaagcggaac agattttccg gtgatgtaag cctatgtcca tcctttacag
    18421 gtgcggaaag ctccggcttc atattggtaa ctgtacgata taataagtta ttccctgctt
    18481 tttcatctgc aagacggaag tactgttctc caatagcact ggtctctgat gtcattttca
    18541 ggatctcttc atcataataa tgaataccca actcttctgc aaggcgcata cctgtaatac
    18601 gtccgccgct tccgtactga cgctcgattg tgatcacata atgtttatcg ctcatatgac
    18661 cgacctccct tgttttgcta ccttaagtat agcacatttt tacctagccg gacactgtat
    18721 tatctgaaat ttactgaaat ttttcttcaa atttctcata acaacaggtg caacggatac
    18781 agctttgcaa aaaacagttg acaaaacccg ccctatgtat taagatacgg attaacagct
    18841 aaaccgttga gaaagaggag taagcttcca atgcattagc acagagagcc gcaggtggtg
    18901 ggattgcggt ataaggcagg cagctgaatg gacttttgag ggcagtcttg aaatatttca
    18961 gtagggactg acggactctg cacccgttac atttgcagca catatgttnn nnnnnnnnat
    19021 gttagtatgt gaaaagtgga ctttcagtca atttgagtgg caccgcggat catattcgtc
    19081 tcaatcacaa tcgtattgtg gttgggactt tttttattcc aaaatattat ttcaaatttt
    19141 attttcagga ggtaagtatt atgaaaaaat ctatggcatt ccttttaacc gcagcaatgg
    19201 gcgcagcttt attaacaggc tgcggaagct cctcttctac tgctactaca gcagcagata
    19261 ccacagcagc cccttcttct gaggcagcat cctccgagga agcttccagc gaagcagaaa
    19321 acaccgctga tgcagacggc tcctacacca tcggtatcgg ccagtttgca gagcacggct
    19381 ctcttgacaa ctgccgtgaa ggtttcttag aaggccttgc agcagaaggc attgaagaag
    19441 gaaagaactt aaccgttctt tatcagaatg cacaggctga cggcggtact tccgcacaga
    19501 tcgccaatac atttgcagga aagaacgtag atttaatgtg tggtatcgca actcctatgg
    19561 ctcaggctga atatagcgtg gctatgaaat ctgatattcc tgttatcttc accgcagtta
    19621 ctgacccagt tgcagccgaa ctggcaaatg cagacggaac cccaatcggc gagatcacag
    19681 gtaccagcga taagcttcct gtagagcagc agttaaagat gatccgcgag atcttacctg
    19741 atgcaaagac catcggtatc atgtacacca ccagcgaagt aaactctgag tctgcgattg
    19801 cagaatacaa ggaactggct ccaaattatg actttgagat cgtagactcc ggtatctcct
    19861 cttctgcaga tattcctctg gcagcagata ctttaattgg aaaggttgac tgtattacaa
    19921 atctgaccga taatacagta gtagcatctc ttcctgttat cttaagcaag gcttccgcaa
    19981 agaacattcc tgtattcgga agtgagatcg agcaggtaaa gatcggctgc ctggctgcta
    20041 tgggtcttga ttatgtagaa cttggcaagc agaccggtga aatggctgca aaggtattaa
    20101 agggcgaagc aaaagcaagt gaattaaact ttgagaccat taaggatgct gcattctacg
    20161 gcaacaccaa agtagctgaa aaccttggca tcactcttcc tgagtcttta acaggaaatg
    20221 ctgctgaact ctttgacagc attacagagt aattttagta ggaacacaag tattggagga
    20281 acatcatgac aacgatcatt ttcggtattc tggaagaagg ccttgtatat gccatcatgg
    20341 ctctgggcgt ttacatcact tataagatcc tggacttccc ggatctgtct gtagacggca
    20401 ccttcccact tggcgctgca gtgactgcag caggcattgc aaacgggctt cctttcattg
    20461 gtaccatttc acctgtagca gcattattca tttcctttac agtgggtgct ttagccggct
    20521 gtatcaccgg tctcatccac gtaaagttga aagtacgtga cctgctctcc ggtatcattg
    20581 tcatgacggc cctctattcc attaatttaa ggatcgcagg aaaagcaaac cttcctattt
    20641 ttagtaagga aaccatcttt tccaattcct tcctttccgc tcatgtgccg gaagctgcat
    20701 ccccatttat cgtgaccatt atcctgtttg tgatcgttat gatctgcaag gtgttattag
    20761 acgcttactt acagacacgt tccggatacc tgcttcgtgc agttggtgac aatgacgtac
    20821 tggtcacttc tcttgcaaaa gacaaaggcc ttgtaaagat cgtaggtctt gctattgcaa
    20881 acggttttgc agctcttgca ggaagcgttt actgccagca gaaaggcttc tttgagatct
    20941 ccatcggtac tggtaccatg gtcatcggcc ttgcaaatgt gatcatcgga acccagctgt
    21001 taaaaagagt tggatttatc cgctctacta ctgctgttat catcggttcc attgtataca
    21061 aagcctgcgt atccattgcg ctgctgttaa atgacctgca cattgcagga ctggaccttt
    21121 ccattcctgt cactgcatcc gacttgaagc tgatcacttc tgtattattc ctgatcatcc
    21181 ttgtagtaag cccttccggc ggaaagaagg taaagagcca tgcttgaatt aaaacacatc
    21241 cataaatatt acaacgcagg tacagtaaat gaaatgtgcc tgtttaatga ttttaactta
    21301 accattgaag accaccagtt tgtatccgta gtaggaagca atggttccgg taaaacttct
    21361 cttttaaata tcatctgcgg aagtatcccc ttagatgacg gccgtattat catcggtggc
    21421 gaagacatta ccaaaatgcc ggaatacaag cgtcagcgcc gcattggacg cgtttaccag
    21481 aacccgtcta tgggaacctg cccttctatg accattgcag agaacatggc tttagcagat
    21541 aataagggca aagccttcaa tctccgtcct ggaaccaaca aacagcgcct ggaattctac
    21601 cgcgaatctc tccgctccct tggccttgga ctggaagata aaatggacgt aaaagtaggc
    21661 gttctctccg gcggacagcg ccaggcaatg gcacttttaa tgtccactat gactcctatt
    21721 gaattcctga tcctggatga acacactgct gctctggatc caaaaactgc ggaaacgatc
    21781 atggaactga ccaacaaagt agtccgtgaa aagaacctga ctaccattat ggtcacccac
    21841 aatctccgct ttgctgtgga atatggtgac agactgttaa tgatgcacca gggacatgca
    21901 gtgatcgata aagcaggcgc agaaaaagac gctactacca ttgatcatat ccttaagaaa
    21961 tttaatgaga tcagtattga gtgtggaaac tagatagcaa agggacgcgc caaccggtgc
    22021 gtcccttaat tctttcattc aatttttatt ctgcaaatac catgatccgt gttgctgtat
    22081 tctctccatc agaccatacc actgcttttc tgccctgagt caggtcatct aacgtaacaa
    22141 tatttctggt aagatacggt acgatcccac agtcatctgc cagtgtatat tctgtgccgt
    22201 ccatagcagt cagtacagac tgggaagaag ctgcatctgt cactactgat ttaactgtta
    22261 catagtccgg aaccttaaaa tctgccggaa cattggcaaa gatcatcgtc gcattggtca
    22321 ttggcggaag gctcatggtc attgccggtc cgatgtaagc gtagatggtg tctccgtctt
    22381 taatatcaga aagttctatt ggaagaccgg aaactgcgtc taatacataa gtagactcct
    22441 gggaaacgtt taagatcact tctccctgat atccctgatc tgcctgactg tcaatggata
    22501 atctgccatc ttccatctgg gtagccggtc caaacaggca tacagagtct tctgcctgct
    22561 ctggctgctc ttctgcactt tcgatcgtgc cgcttacagt ttctacaacg gtagtctctt
    22621 ctgcagaaga agtaggttct tcagactcta ccaccactgg tcttgactca tttacaagag
    22681 gcaatgtggt tgccgcatcc actttattgg agcatccgcc aaggcctaaa actgctgcgc
    22741 tgcataatac tgctgcgtat aaatgatatt ttctcaatat gatttctcct tttctataac
    22801 aaagatcatt tctttaactg tgctctaata ctcaggttcc cgtgctgacg cgctccactg
    22861 cctgctgacg caggcggaac cttcgttaat gacttgagaa aaacaaatgt ctgctgacgc
    22921 agccgcttgt ttttctctgc gttcattata tactatattc cccttacatc caataaagct
    22981 tttcttaagt cttttttaaa tctctacaac aaaacaggag ccttcccata taattacagg
    23041 aaagctcctt tttttatata caatatagtt ttgaaacctt ttaacaggct tttattatct
    23101 tgccatctca ctctgtggtc cggtctgatt atgggccttg ataatggtct cgatttcttc
    23161 caggttagag gatggaagat ctccaggaat ggtgttcttt acagctgctg tggcgtttcc
    23221 aaaagcaact gccttctggc agtcaccacc gctgcttaac aggccgtaga gagcgccgga
    23281 aatataagca tcaccactac cgatacggtc tactacttca atgtcacgat atggctcttc
    23341 ttcgtagaaa gtatctcttg ctgcatcata gatcacagaa ccaaaggtat ggcgtttcgg
    23401 gctgtgtacg atacgctggg tggaagctac gatagagatc ggatattctt ctgtaaagct
    23461 cttcatcatt tcttttgctg taccagtctt taagaaggtc agtcttgcag tatcttcaga
    23521 gcagaagaag atatctacat aaggaaggat acgctcaatg cattctcttg cttcatcacc
    23581 cgtccacaag tttccacgga agtttacatc aaaagaaacc agggttcctg cttctttgaa
    23641 acgtttgatc atctcaatgg tagtctctct gatcttctca ctaagagcca gagtaatacc
    23701 ggtggtatgg aaacacttgg aagctgtgta tacttcctcc gggatctcat caatatccag
    23761 agtaaagaaa gaactgttca tacggtcata aataaccttt ggttttcttg ggtatgcacc
    23821 gttttcatag taataaatac ctacacgggc tgatggagaa tgatcataca taaaaaagtc
    23881 atcactgaca ccaaaggaac ggatcttacc tttcataaag ctgccgatat catgtgacgg
    23941 aagtttggag ataacaccgg tatgaaggcc taacagagac gcaccaactg ctacgtttaa
    24001 ttctgcaccg cctacctgtt ttacaaatgt atcacctctg gaaagacggt cattgtctgg
    24061 tggtgacagt cttaaaagaa gctgacctaa tgtcagcagg tcaaatgttc ttccttttat
    24121 ctctcccatt ttcatatcct cctgaagttt aaaaaatttt cataaacgta ttattaaaga
    24181 aaatttctaa agaaaaggcc tgtctgaaaa tacattccag gaacgctatt tccaaacagg
    24241 cctttcggtc ttaattttta actcttaaga agagtaatta tctctgtaca cgacctgaac
    24301 cgtcgccgcc tgctaaggtt tcaacttctg cacgggttac caggttgaag tctccaggga
    24361 tggtgtgctt taatgcggaa gctgctactg cgaattccag agctgccttc atatccttgc
    24421 catcagccag accgcagatc agaccacctg cgaaggagtc gccgccgcct acacggtcaa
    24481 cgattggagc gatgtggtac tttctggagt ggtagaattc acgagtctgt ccgtccatga
    24541 tgcaagcaga ccagtcattg ttggaagcgc tgtggctttc acgtaaggag ctgatgatgt
    24601 acttaaatcc gaacttgtca gccatctgat taaagatgtc aacgtatcca gctaattcca
    24661 gttctccgga agtaacatca gtgttgcctg gcttgaagcc aagaaccttc tctgcatcct
    24721 cttcgttacc gatgcaaaca tcaacatact gcatcaggtt ggtcataacc ttctgagcct
    24781 tctcggaaga ccacagtttc ttacggaagt ttaagtctac ggaaacagtt acgccgtgag
    24841 ccttagctgc ctttaaagct gcctcagtca gttcgattgc tgcatcgctg acagctggag
    24901 tgataccggt gaagtggaac cagtcagcat cagcaaagat ctcatcgaag ttgaactcat
    24961 ctgcagttgc agtagagata gagctgtgtg cacggtcata aactacgttg gaagctctca
    25021 ttgcagaacc ggtctctaag tagtaaattc ccagtctctc accgcttctt gcgatgtact
    25081 ttgtacctac attgtatttt cttaaagctg ctactgcgca ctcaccgatt gggttagctg
    25141 gtactgcagt aacgaactgt acgtcatgac catagttagc taaagataca gctacgttag
    25201 cctcgccgcc gccgtagttt acatcaaact catctgcctg gataaatttt tcattatttg
    25261 gggtagataa tcttaacatg atctcgccca tggtaacaat ctttgccatt ttaatattct
    25321 ccttaagata tgtgaaaatt gatattacag ttcagcgcat ggctgaactg taagaaattt
    25381 attaacaatt atgctcttgc tgctgctaca gccttaacga attcctgagc ctttgcagtt
    25441 acagctgcga aatcaccagt cttagcgccc tttgtaaggc tgcttccggt accaactgcg
    25501 tatgcaccag catggatcca cttatcaacg ttgtctacgt caacaccgcc ggatggcatg
    25561 aactcaccct gtggcagagg tcccttgaag gagctgatag ctgcaggtcc tacgatattt
    25621 cctgggaaga tcttgattac atcgcatcca gcttccatag cggtaatagc ttcgcttgga
    25681 gtcataacgc ctggcagcat tggtactctg taacggttgc aaagcttgat ggtctctaca
    25741 tttaagcctg ggcttacgat gtagttagca cctgctaaga tgcaagctct tgcagtctct
    25801 ggatccagaa cggtaccagc accgatcacg atcttgtcat tgtctttata cttgtcagcc
    25861 atcttcttga tgaagcctac tggatcaccg tcatccatag tcatggtcag ctcgataaag
    25921 ttgatgccac cttcaataat agcatctact accttctcac cctgctcata attctttgca
    25981 cgtactacag caaccaagca gtcttctttc agtcttgcta aaacatgttc ttttttcatt
    26041 tttcttctcc ttctttcgat gaggttcgta aatacgaatt aatttcatat ttacgattta
    26101 tacttaaatt ttaatagtta ccaactgatt tgtcaacagc aaaacagata aattattatc
    26161 ttttatcctg ttttatttac ccaaaaatcc caaaagtctt gaaagcgctt ttgccttctc
    26221 gcaaaccagc cttcctaagg cgtcataatc ctctgtaggc ttgtaaagac tggacacact
    26281 gatggccccg atcacattcc catccgggcc gaaaacaggg gaacctacac attccatatg
    26341 ttcctccaac tcccttgcgt caaaggcgta tccattttcc cggacccgat ccagttcttc
    26401 tatgaaatga gtcttgtcct gaatggtacg ctgggtatgg ggacggaaat tgatccggtc
    26461 tgctaaggca tcccgctctt cctgctccat atttgccata atggcctttc caagggaagt
    26521 acagtacaat gggttcttgg ttcctacagt cgctgttgtg ataatgggat tttccggctc
    26581 aaatttacag atgtaaacaa tagagtcatc tgaacgcact ccaaagaata cggtctttcc
    26641 cacttctttc gcaaaagctt ttaatacagg atcaatggta ccgataaaat ccaggttatt
    26701 tgtataatta attcctacac gataagcagt aagtccgatg gtataacgct gcttctgccc
    26761 ctttaccaca tggaccatac ctgtatgtgc cagtgttgtg ataatgtcat aggcgctggt
    26821 ctttggaagt tctaacttct cacagatctc atccagtgta ataccatccg gctttttgga
    26881 aaccattttc agtatatcca ctgtgcgcag cgttgtccgg tttaatttca tttatgttcc
    26941 tcctgcatca ggttctgtcg atcatatatg tcttttgtat ttggtcgtat atatgtttct
    27001 tatacgcata tacgattttc ttcttttagt atagcgcatc ctgaaacaga ttacaagatg
    27061 cgcttttgtt ttcctggcac agtcagatca aattccgttg ccggtcatgc aacgcatgaa
    27121 ccgtaactgt tttgttcact aatacttaca gagttacacc ctgtttgaag attgccatat
    27181 catgaaaacc ggctgcttct gatttaacct tgcgtccgga agcaacttcc aatacatagt
    27241 ttaacagatc ctgtgccagt tcttctttgg tcttatcctc taccatctgt cccgcattga
    27301 aatcgatcca gttggacttg tgtcctgcaa gcttggagtt actggagatc tttacagttg
    27361 gaacagggga agcaaacgga gtgccgcggc cggtagtaaa caataccata tgagcgccgg
    27421 atactgccag cgcagttgct gctaccaggt catttccagg tgcgcttaag aggtttaatc
    27481 ccttaacctt aactggctcc gcatactcta atacaccctt tactaaagaa cttcctgact
    27541 tctgggtgca tcctaaggat ttatcctcca gagtagagat accgcccttc ttgttacctg
    27601 gggatgggtt ctcataaata gtctggttgt ggctggtaaa gtagttcttg aagttgttga
    27661 tcagagctac ggtcttgtca aaaagctccg gagttgcgca gcggttcatt aacagagtct
    27721 ctgcaccaaa catttccgga acctctgtta aaatagtagt tccgcccttg cttactaaca
    27781 gatcagagaa agcaccaact acagggtttg cagtgatacc ggaaagtccg tcagaaccac
    27841 cgcacttcat accgatgatc agttcgcttg cgtcgatctt ctctctggag aatgcgtttg
    27901 cataggttgc cagttcttcc aggataccca tagcggtctc catctcatct tctacatcct
    27961 ggcactgtaa gaatttaaca cgctgctcat catactcgcc aatgtaatcc attaatacag
    28021 gaatattgct gttctcgcag ccaagtccta ataccagaac acctgctgca tttggatgat
    28081 ggatcatgtc tgccagaact tttctggtgt tttcctggtc atcacccatc tgggaacatc
    28141 catatggatg ctggaatgca atgatatctt ctacatttcc gcctacaaac ttctttgcag
    28201 cagtctcaag agcctttgca atagagttta cacagccaac agttggaacg atccagatct
    28261 cattacggat accgactttt ccgtctgctc tgcggtagcc gtcaaagtat acatgcccag
    28321 atggctctac gtccttgctt ccttctggtt tatactcata agaaagaaca tcgcctaaag
    28381 cggtctttac attatgaaca tgtacccatg cgcctttttc aatagcttct tttgcgtagc
    28441 cgatacggaa accgtattta actacctctt caccctgtgc aatggcacga agggcaaatt
    28501 tatgtccctg tgggatatcc tggaccagag ttaccttttc tcctgctaca tccagggtct
    28561 ctccttcatt taaaggacga agagcaacgg caacgttatc attctgattg atcttgacaa
    28621 tgtcctgcat ggtctttaat ctcctatctt tgtttttgta ctcaggaaac cttcccaaaa
    28681 taaaatctgt tgcaaaagat tccggaatac atttgattta ttttaactac agatcacttt
    28741 gtaagtgctt acaatcattc tacctatttt ctacacagaa gtcaatagaa aactgttgca
    28801 atctttatta gtagtatgta taatagactt agcgtgtaag cgcttacaga atgtgaggtg
    28861 gattactgaa tgaatatata tgacgtttct gagcgcgcag gcgtttccat tgctacggtt
    28921 tcccgtgtac tcaacggcaa tcccaacgta agtgaacgca cccgcaagaa ggttctggct
    28981 gtaatggatg agttgggtta tacccccaat gtttttgcca ggggactggg acttgatacc
    29041 atgcagacca tcggtatcat gtgttctgac tcttctgatc cttatcttgc tgaggcgatc
    29101 tactatctgg agcgggaact gcgcagtcat aattatgatt ctatcctctg ctgtaccggt
    29161 tacgacctgg atgtaaaaca gaagtatttt aatctgcttc gctccaagcg cgtggatgct
    29221 attatcctgg caggttccaa atttgtggaa atgcgttccc gggataataa ttatattata
    29281 gaagctgccg cagacggact tcctgtaatg ttagtaaacg gttatctgga aggaaagaat
    29341 atctacagta ctgtctgtga tgattatgcc tctatgtatg atgccacatt acagctgatc
    29401 cattcaggac acagccgtat cctttatctt tacaccagcg tttcctacag cggtgttaat
    29461 aagatggagg gctacaaagc agcattgaaa gaaaatcgta tcccggttga ggactccatg
    29521 attcatcaat gttccaagag ttttgaaaat tccagggatc ttcttttaag cctgaaagaa
    29581 gaagggctga aatttgacgc agttttggct tctgacgatt cactggccgt aggagccgta
    29641 aaatatgccc atacagccgg tatttccatg ccggaagagc ttagtattat cgggtataat
    29701 aattctctcc tggcacgttg tacagatccg gagatcacat ccattgacag caaggtagaa
    29761 gcactttgca ccactactgt caatacttta atgggggttt tcagtggcgc taatgtccca
    29821 aaccggacaa ccatcgcctc tgctttaata aaaagaaaaa ccacaaactt ttaaattcca
    29881 attttaaagg aggatttacc actatgaaac agtttttgga caaggacttc ttattatcca
    29941 cagacatggc aaaaacaatc tatcatgatt acgcagaaaa tatgccgatc ttagactatc
    30001 actgccatat caacccacag gagatctatg aagaccgtaa gtttgaaaac attacccagg
    30061 tatggctggg cggcgaccat tacaaatggc gtcagatgcg ttctaacggt gttgatgaga
    30121 aatacattac cggcgacggc acagacagag agaaattcca ggcatgggct gagacaatgc
    30181 ctaaactgat cggcaaccca ctgtatcact ggagccactt agagttaaga agatactttg
    30241 gatacgaagg ctacttaaac ggcgatactg ctgaggaagt atggaacctg tgcaacgcta
    30301 agttacagga agactccatg actgtccgta acctgatcaa gcagtctaac gttactctga
    30361 tctgcaccac tgatgatcct gttgactctc tggaatggca caagaaactg gctgaagata
    30421 ctacttttga tgtacaggtt ctgccagcat ggcgtccaga caaggctatg aacgtagaga
    30481 agc
//
LOCUS       DBMDUA010000010        29383 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1160_length_29383_cov_3.946862, whole genome shotgun sequence.
ACCESSION   DBMDUA010000010 DBMDUA010000000
VERSION     DBMDUA010000010.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 29383)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 29383)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29383
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1160_length_29383_cov_3.946862"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(258..1298)
                     /locus_tag="ACZPCL_01660"
     CDS             complement(258..1298)
                     /locus_tag="ACZPCL_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotS"
                     /protein_id="HIR1161231.1"
                     /translation="MNERYQEVLEQYEVEILSVRKGRDAWICETDKGLKLLREYKGTV
                     RRLEFEDQVLECLDTRGSLRADQYVRNQEGALLSKAGDGTGYIMKEWFSDRECNIRDS
                     YEIRQGISRLAMLHGQLRQIPVREEWNMGSICIESLEKEQERHVREMKRARNFIRGKR
                     GKTEFELCVIDSFDHFFDQAKEAVRQMNSLFPENGITDRLEAAELAPEGSGENQGYLC
                     HGDLDQHHVLMGNGYTAIIEYNRMHLGVQAEDLYRLMRKVMEKHSWDCDLGISMLDSY
                     ERVRPMDKKERKCLYYMFLFPEKYWKQLNFYYNTNKAWIPAKSTDKLKSLKNQEGARG
                     RFLSRLEAVLMS"
     gene            complement(1485..2003)
                     /locus_tag="ACZPCL_01665"
     CDS             complement(1485..2003)
                     /locus_tag="ACZPCL_01665"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIR1161232.1"
                     /translation="MMNVVLLEPEMPSNTGNIGRTCVATNTRLHLIEPLGFKLNEKAL
                     KRAGLDYWDKLDVHVYSDYQDFLEKNPDALGKMYFATTKAHKVYSEVDYEPDCYLMFG
                     KESAGIPEEILVENEDHCVRIPMWGEIRSLNLSNSVAVVLYEALRHNDFEKMALEGQL
                     HHMKWKDGWEQK"
     gene            complement(2008..3180)
                     /locus_tag="ACZPCL_01670"
     CDS             complement(2008..3180)
                     /locus_tag="ACZPCL_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1161233.1"
                     /translation="MRNPLSDKIVSIPPSGIRKFFDIVNEMEGAISLGVGEPDFDTPW
                     HIREEGIYSLEKGRTFYTSNAGLKQLKDEICNYLKRRFEVSYDPDTEIMVTVGGSEAI
                     DVAMRAMLNPGDEVLIPQPSYVSYLPCVTLADGVPVIIELEEKDQFKLTPEKLLEKIT
                     DKTKILVLPFPNNPTGAIMEKHELEEIAKIVIEKDLFVVTDEIYSELTYQRKHATIAA
                     FPGMKERTVLINGFSKSYAMTGWRLGYAAAPDLILKQMLKIHQYAIMCAPTTSQYAAV
                     SALKNGDPDVEMMRESYDQRRRFLLNAFREMGIDCFEPLGAFYTFPNISRFGMTSEEF
                     ATKLLMEEKVAVVPGTAFGACGEGFVRISYAYSLKSLKEALSRIERFVKRHDGKAE"
     gene            complement(3177..3659)
                     /locus_tag="ACZPCL_01675"
     CDS             complement(3177..3659)
                     /locus_tag="ACZPCL_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR1161234.1"
                     /translation="MREKILAVIEKNSRIDIKDLAVLLGESEIAVANEIAEMEKEHII
                     CGYHTLINWDNTSEEKVVALIEVKVTPQRGMGFDKIAERIYQYNEVNAVYLMSGSFDF
                     TVFIEGKTMRQVAQFVSDKLAPMESVLSTATHFVLKKYKDHGTIISEPVQDERMLITP
                     "
     gene            complement(3707..4312)
                     /locus_tag="ACZPCL_01680"
     CDS             complement(3707..4312)
                     /locus_tag="ACZPCL_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HIR1161235.1"
                     /translation="MRTAGQGPGGFEKRDGFIKPGQDLVVAGFAGLAGTRQIFQKKRE
                     KLEGHFAPSFLRCLEEKDSYNVKEWLENELKQKNCPVTAWEYAGEGGILTAVWNLSGI
                     YNVGVDIDLRMIPMKQAVVEVCEIFEVNPYRLFSENCVILACDRGGQMVRSLSAQGVP
                     AAVIGSAEKGIARVIRHGEETAGYLERPRADELSRIDGNTI"
     gene            complement(4309..5886)
                     /locus_tag="ACZPCL_01685"
     CDS             complement(4309..5886)
                     /locus_tag="ACZPCL_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR1161236.1"
                     /translation="MGNRTLKAGLFAALAAGALWVLPMSAWAAPVFPEGISADGESLA
                     GKTWEDALHTAEEKVKDQAGISVALTIEDKKAETTAGELGFHWSNQDEAEKELKSYVG
                     GSLIRQYMNKKDLEKAPVDVSIKTAADPDKIRDFVDTHCDGVLAQPQDASIRRENGAF
                     VITESVLGKVVDADATASALDTAFEGLKDSNGEISVQAVIIEEQPAITSDDLKTIEDV
                     LGTCTTDFSSSGAARSTNLAVGAGKINGRVLMPGEVISGYECLQPFTLENGYKTAAAY
                     ENGQVVDSIGGGVCQIATTLYDAALQAEMEIVQRQNHSMIVTYVKPSMDAAIAGTYKD
                     IKIKNNYSTPVYIEGYTSGKKLTFTLYGKETRPSNRQVEYVSETIGTTNPGEPQMITD
                     KSLAPGAKVKVQSAHTGYKSRLWKVVTVDGVEQERTLLNEDTYNASKAIYRAGPAHAA
                     PAPVPEQTAPATTPETAENHTPETAQTEPAQTEAEHKAVTGENGGPGVVPTTAAQPAG
                     DNAGAESPASPAQEENP"
     gene            complement(6062..6784)
                     /locus_tag="ACZPCL_01690"
     CDS             complement(6062..6784)
                     /locus_tag="ACZPCL_01690"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR1161237.1"
                     /translation="MTDSYIALDLETTGLEPKKEKITEIAALKVEHGQITGRFVTLVN
                     PKRPLTERVVELTGITDDMLKDAPVIEDIIGEVLEFIGNLPLLGHNIRFDYSFLKQAA
                     VNQKMEFECEAVDTLKLCRKLMPEQEKKNLGNACTYYKIEQPLAHRAEADALSAHFLY
                     QKLVALHGEEQPELFVTTPLNCRMKRQQPATKRQKEYLQDLLKCHRIDITVQLDDISR
                     SDASRLIDKIILQYGRVKPQNK"
     gene            complement(6840..7520)
                     /gene="nth"
                     /locus_tag="ACZPCL_01695"
     CDS             complement(6840..7520)
                     /gene="nth"
                     /locus_tag="ACZPCL_01695"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR1161238.1"
                     /translation="MIKMTEKLKERISHILSVLDKEYGTDFICYLNYETPWQLLIAVI
                     LSAQCTDARVNIVTADLFKKYPTLEDFAAADLKELEQDIHSIGFYHMKAKNIIQCAKR
                     LVEEYGGIVPERLEDLLTLAGVGRKTANVIRGNIYHVPSIVVDTHVKRISRKLGLTKE
                     TEPEKIEADLMKVLPEDHWILWNIHIITLGRTICTARKPDCGNCFLKQWCPACEVPAV
                     FKETEKID"
     gene            7663..8511
                     /locus_tag="ACZPCL_01700"
     CDS             7663..8511
                     /locus_tag="ACZPCL_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1161239.1"
                     /translation="MQSKKDLYQIGEVARLFHLSISLLRHYDKTGLVTPEYTDPDTGY
                     RYYSIRQFECLNTIRYLRVLDMPLEEIGDFLHNRDTGRIQELLSKQLVQVHKKQQELA
                     VMEHQIKNRLNQIQGALSAHPGEIFLEKKPPLKLACMKKQLNPENYLDLEYSIREMEQ
                     GNSPASIFLGKVGVGLFYESLKAHRFKPYDFVFIILEEDDPIKGQVIHIPESTWAIVR
                     FHGSHEQAPGFYEKLLAYFKDEGFAPESFALEMTLIDYGLTHDQSQFITEIQIPLNPD
                     CPGIIA"
     gene            complement(8479..10248)
                     /locus_tag="ACZPCL_01705"
     CDS             complement(8479..10248)
                     /locus_tag="ACZPCL_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR1161240.1"
                     /translation="MKVLLAAINAKYIHSNLGIYSLKTYGEKMLKEWGLAEQAEISLA
                     EYTINHQMEQILQDIYKRKPDVIGFSCYIWNISYVKMILADIKKVLPDVKIWAGGPEV
                     SYHGEAFLKEEPAVDLVMMGEGEITFAHFLKALLEGEDLKQVPGLMVRNADGTFTNTG
                     FRQVMDMSQIPFPYAFMDMKELEHRIIYYESSRGCPFSCAYCLSSIDKKLRFRSLDLV
                     LPELEWFLQAKVPQVKFVDRTFNCKKSYAMAIWQYIRDHDNGVTNFHFEIAADLLDKD
                     ELDLLSTMRPGLVQLEIGVQSTNEKTLEAIRRKTDIEEIREITETINSWHNIHQHLDL
                     IAGLPWEDLESFKKSFNDVYEMEPEQLQLGFLKVLKGSYMEELIPDCDLLYSAAPPYE
                     VLCTKWLSYGDVLELKDIEEMTEVHYNSRQFTCTLKELEKEFDTPYEMFSFMAGYYNK
                     NHLFGISHSRIARYEILWKIIQERLEKNGKCETQAMAENVNISEKLELYRDLLMTDLY
                     LRENVKSRPTFARDLSSSKDFVREFFQKEEKTPEHLSGYEGYDSRQMAKMAHLEPLRD
                     GTYLLFDYKKRDPLSYNARTVRV"
     gene            complement(10250..10744)
                     /locus_tag="ACZPCL_01710"
     CDS             complement(10250..10744)
                     /locus_tag="ACZPCL_01710"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIR1161241.1"
                     /translation="MSDKRVDYITWDEYFMGVATLSGRRSKDPSTQVGACIVSQDNKI
                     LSMGYNGFPKGCSDDEFPWGKEHELDDPYNAKYFYSTHSELNAILNYRGGSLEGSKLY
                     VTLFPCNECAKAIIQAGIKTIIYGGDKYAHTPAVKASKRMLNAAGVRYYQYQPTGRKI
                     EIEV"
     gene            complement(10947..11324)
                     /locus_tag="ACZPCL_01715"
     CDS             complement(10947..11324)
                     /locus_tag="ACZPCL_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HIR1161242.1"
                     /translation="MTREFTCIICPNGCEISADYEIQADQSVVIRSLEGATCKRGAEY
                     VKQELTDPKRNIATSVLVEGGELPLASVRLTAPIPKARIFDAMDEIKKCRLKAPVKAG
                     TVVIKGIVGLDADVIVTKNVAKA"
     gene            complement(11321..12580)
                     /locus_tag="ACZPCL_01720"
     CDS             complement(11321..12580)
                     /locus_tag="ACZPCL_01720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1161243.1"
                     /translation="MEHKTEMWEIEQADAVIVGGGPAGLAAAVRLYENGITDILILER
                     EKQLGGILRQCIHDGFGLARFKTTLSGPEYAQKFIDLANEKKIRYLTDATVLEISEDK
                     VITVATPDGLKQWKAKAVILAMGCRERTRGALGIPGERPTGVFTAGVAQAYMNLYNRM
                     PAKEVVILGSGDIGMIMARRLTLEGAHVKAVFEIQPYPSGLPRNIEQCLNDYNIPLYL
                     SHTVTEIHGDKRLTGVTVSKVDEHMRPIPGTEEEYSCDTLILSVGLIPENELSLDAGV
                     TLDARTKGATVDEYFQTDRPGIFAAGNVLHVHDLVDFVSMEAEKLADSAARYIKEGKL
                     PACEIQVKTDKNINHTVPQRISGTEDCCLSLRVNRPFKDCALVVSQNGKEIARKKMKK
                     ALPAEMIHIDLKAEKLENKGDLEVKVE"
     gene            complement(12589..14046)
                     /locus_tag="ACZPCL_01725"
     CDS             complement(12589..14046)
                     /locus_tag="ACZPCL_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1161244.1"
                     /translation="MKEMYDVLIIGGGVVGSAMAREMSRYRLKIGVLEKNLDVCNETS
                     GRNSGVVHGGFAYDRGSLKAKLCVEGNKMMGDLAKELDFHFIRCGKVLVGNTEEDMET
                     LKRTMEQGKDNGATNLELIDEKRLHELVPAVVGKFAMFSHNSGIVDPFGYTIALAENA
                     HANGVEYHFGCEVTAIRREDDDTYEITTTKGNFKTRWVVNCAGLGCGKISDMLGITGY
                     RIIGSKGDYIILDKRTGPLLPMPVYPVPSNTYMGIHVTNTTDGNVIIGPNADMVTDFT
                     YYGVPQKNMDYLAESASDLWPCIHKADYIRNYSGILPKWVDEKGVIQDFKIEIKDEIA
                     PHALNLVGIESPGLTAAVPIARYAIELMKERETLEENPDFNPVRKGIRHFAQCTKEEQ
                     AELIKENPDYGEVICRCEKVTKAEILQAIHNPLGVDTMTGVKYRTRSMMGRCQGGYCQ
                     MRIEQLIEQELGKKETEVQYEREGSNVLFGKVREV"
     gene            complement(14327..14758)
                     /locus_tag="ACZPCL_01730"
                     /pseudo
     CDS             complement(14327..14758)
                     /locus_tag="ACZPCL_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588440.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(14933..16015)
                     /locus_tag="ACZPCL_01735"
     CDS             complement(14933..16015)
                     /locus_tag="ACZPCL_01735"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR1161245.1"
                     /translation="MERTKGLKEYIQTKEYRESLGLSGQVTETYTMLAQGEYNRNYTF
                     THPITGQKLVLRINFGSQMHLKDQILYEYRALKLLENSGRTPKAIYADGSCKNLPYGV
                     MVMEFLPGHSLDYKTELFSAAGCLADIHSVKMPETHSLIQPKEPLKAILEECEAMVKT
                     YMESELGDEQKKRRIRSLLDQGWKAVESLETDIPYHCCINTELNSTNFLVNDRNVDGR
                     RINENSVGEKTGDCIKDNEKKAYLVDWEKPLYGDPAQDLGHFLAPTTTFWKTDVILDQ
                     DRIDAFLDTYIEKVNGRFDTTGLKERTYIYIPVTCLRGIIWCAMAWVQYQQPDKEIFN
                     QSTFDKLEVYLSDEFLSRIENIWNRF"
     gene            complement(16030..17364)
                     /locus_tag="ACZPCL_01740"
     CDS             complement(16030..17364)
                     /locus_tag="ACZPCL_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR1161246.1"
                     /translation="MKQALILFTRIPVPGKTKTRLMPYFSPKQCAKLHTCFLKDISEQ
                     CRKVKADCFVCYAPEDGDVKELKDILGKQKEYFPQAGESLGQRMYHSLEYVLGLGYDV
                     CLLMGTDIPEVSSPDLEKAFQVLKEKDAVFGRTADGGYYLVGMKRPIPEAFGLKQYGH
                     SHVFEDTLKELSDAGINVGFTTKHQDMDTPEDLQCYRQRMRQDRRLQNSHTGRYLAAN
                     VKISVIIPVYNEAKTIEAMKKQLYPYRSRCEILFVDGGSTDGTPEMIGPDFKLLRSEK
                     GRANQMNLGAEESHGDILFFLHCDSELPPRPLEEIRRVMKDHSAGCFGIAFHSGNFFM
                     FTCRVISNHRIKDRKVMFGDQGIFIDRKLFLQAGKFPVIPVMEDYQFSLTLKEMGVRL
                     GMAKKRIYTSDRRFPKGTIPKLKLMWKMNRLRKMYRDHVPIEKIDRLYRDVR"
     gene            complement(17365..17793)
                     /locus_tag="ACZPCL_01745"
     CDS             complement(17365..17793)
                     /locus_tag="ACZPCL_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HIR1161247.1"
                     /translation="MTKEEVCQLFMAGIDCSQVVTGACAEKMGMTKEQARKMSACFGG
                     GMMCGETCGAVTGALMVLGMAFGHSEENDGDQKGIMAGKVAEFKKRFLEKYPSDMCRE
                     ILGHDISKPGEMDKVLEKGLMFDLCPQIVLDVLSILEELV"
     gene            complement(17798..18502)
                     /locus_tag="ACZPCL_01750"
     CDS             complement(17798..18502)
                     /locus_tag="ACZPCL_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015544912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR1161248.1"
                     /translation="MKKNKNKWIEKVILIVCVVAAIAVYMAVPSVHQGMNCVFAMFAT
                     GDFTVLRDFVASYGAYAAALSFLLMILQSIIAPLPAFLITFANANLFGWWKGAILSWS
                     SAMAGAAICFYIARILGRDVAEKLTSRSGLAQIDTFFEKYGKNTILICRLLPFVSFDI
                     VSYAAGLTSMSFSSFFIATGIGQLPATIVYSYVGGMLTGGAKLFVTGLLIIFALSALI
                     FMMRSIHMEHMKKKRG"
     gene            complement(18517..19473)
                     /locus_tag="ACZPCL_01755"
     CDS             complement(18517..19473)
                     /locus_tag="ACZPCL_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009214787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR1161249.1"
                     /translation="MKESVTGCTHCGVCTENCEFLKKYDLTIGDTEKLSKMAYHCFLC
                     GKCSKVCPQGIDGREIVLQIRRHRVKEAGGRIPEKGYGMLLWEKEDYKFRRYTGTGKT
                     ALFFGCNFPSFYPETTRYLGKLLAEKADAFSVFDCCGKSIAELGLEEKETVILERLNK
                     KLLEAGVREVVMVCPNCYAFLKDKLSVPVISIYEKLQELGLGNRIMEEQNIFLPCPDR
                     EKRELLKQIRPFLTAEPKILSSANCCGLGGCAALKEPELAAQMAKSAGSIQNTSVYCA
                     SCAGNLTRAGGKNIKHLLVQILGREEVPDVRHSLWNRVRAGR"
     gene            complement(19473..20138)
                     /locus_tag="ACZPCL_01760"
     CDS             complement(19473..20138)
                     /locus_tag="ACZPCL_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR1161250.1"
                     /translation="MILLFLIGKYSGWSGRLLNGDLTQALQHITVENLPAAIFIYLVV
                     TAVGSVAFALPGISFAILAGLLFGPVLGTICCTVAATLGAVAAFLLGRYFLKDTVGAA
                     ARKNPYLRRLLFDETGKKQIWILMITRLVPLFPYNLQNFAYGVTDMPLKTYAVGTFLF
                     MLPGTAMYTFGTAGLADPSIRKACFLGAAILAVAVTTAGVLLKKKVLAEQEKTDLKNG
                     DEM"
     gene            complement(20360..21037)
                     /locus_tag="ACZPCL_01765"
     CDS             complement(20360..21037)
                     /locus_tag="ACZPCL_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR1161251.1"
                     /translation="MKYTEKQTLAEKTAQNLLEMIQEKGYGAGDKLPTEAELVETLGV
                     GRNTVREALRILMSRNIVTIRQGSGTFISDKNGVADDPLGFFMMDDRRQLTEDLLQAR
                     LILEPALAALAAQNGSEEEIKELEYILKELEGLIRDKKDYSEKDSQFHAQIATCSHNR
                     VMTNLIPVITEGVRVFADSVEETEYEQTLLSHRRIFEAIRDGRGEDARQEMYFHLMYN
                     HNRYRKE"
     gene            complement(21065..22174)
                     /locus_tag="ACZPCL_01770"
     CDS             complement(21065..22174)
                     /locus_tag="ACZPCL_01770"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR1161252.1"
                     /translation="METKRIKISDTKNINDEELKEAAQILRDGGLVAFPTETVYGLGG
                     NALDEKAAGKIYAAKGRPSDNPLIAHVSCAAEVTPLVKYIPEAGKKLMDAFWPGPLTI
                     IFPKSDIVPYGTTGGLDTVAVRMPVDPVANRLIALAGVPVAAPSANTSGRPSPTTADH
                     VWQDLNGKIEMIIDGGPVGIGVESTIVDVSGDVPAVLRPGAITMEMLKDVLGEVTIDP
                     AILGPMAAGVRPKAPGMKYKHYAPKADLTLVEPANVAEARKTGEAVAMTQEQVNAMVE
                     TVWKLAKEKIDAGYKVGIICTDESRSHYPEGEVRSIGARKSQASVAHNLYALLREFDD
                     LKVDYIYSESFSQDHLGQAIMNRLSKAAGYQIYKV"
     gene            complement(22350..22754)
                     /gene="atpC"
                     /locus_tag="ACZPCL_01775"
     CDS             complement(22350..22754)
                     /gene="atpC"
                     /locus_tag="ACZPCL_01775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009536610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR1161253.1"
                     /translation="MTTFHLQIATMDGNVFDGQVQSVSCRTIHGDLAILARHMNYCTA
                     IGMGTAHVVLEDGTERKAACIGGMLTMMNGECRLIPTTWEWSEDIDVARAEEAKKRAE
                     DKLSQSGMDDKEVKLAEAKLHRALVRIGTVGK"
     gene            complement(22772..24169)
                     /gene="atpD"
                     /locus_tag="ACZPCL_01780"
     CDS             complement(22772..24169)
                     /gene="atpD"
                     /locus_tag="ACZPCL_01780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR1161254.1"
                     /translation="MTEKHIGKVVQVTGPVLDIRYKEGELPALLNAIEIDINGHKLIA
                     EVAQQIGDDVVRCIAMSSTDGLVRGTDAVDTGSPITVPVGDECLGRVFNLLGDPVDNK
                     PAPEAKERWAIHRQAPSYEEQTPATEILETGIKVVDLICPYAKGGKIGLFGGAGVGKT
                     VLIMELIHNVATAHGGLSVFTGVGERTREGNDLYGEMQESGVIDKTALVYGQMNEPPG
                     ARMRVALSGLTMAEYFRDVKNQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTL
                     ATEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFTHLDATTVLSRDIASQG
                     IYPAVDPLDSTSRILSPETVGEEHYQVARSVQRVLQRYKELQDIIAIMGMDELSEEDK
                     LTVSRARKVQRFLSQSFSVAEQFTGMKGQYVPLKETIRGFKKILDGECDDIPESCFLF
                     AGTIDDVYEKAKQQQ"
     gene            complement(24185..25042)
                     /gene="atpG"
                     /locus_tag="ACZPCL_01785"
     CDS             complement(24185..25042)
                     /gene="atpG"
                     /locus_tag="ACZPCL_01785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009216299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR1161255.1"
                     /translation="MAANMKAVKLRMKSIQSTMQITKAMELVASSKLRKAKERADASR
                     PYFHTLENTLKQIAAGNTDLSSVYTKKSKSEKWCYVVIAGDRGLAGGYNSNLFKEMES
                     ETAGKDYCVLPIGKKAVEYYKRRGIELVTEDFAEAGDISVSDCFEIAALLCRAFKAGE
                     FGHAALGYTNFVSMLSQKPDVSSMLPLEDLSGEEKDMKQIHSLILYEPDSETVFDAIV
                     PEYLAGMVYGAMCESVASELAARRTAMDAASKNAGEMIEQLSLYYNRARQASITQEIT
                     EIVAGAEEP"
     gene            complement(25119..26639)
                     /gene="atpA"
                     /locus_tag="ACZPCL_01790"
     CDS             complement(25119..26639)
                     /gene="atpA"
                     /locus_tag="ACZPCL_01790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR1161256.1"
                     /translation="MQLKPEEISKVIRSQIKYYQNAIEQSETGTVLIVGDGIARVSGL
                     TNCMSGELLEFEDGTFGMAQNLEENSVSVVLFGSDQGISEGQTVKRTGKVVSVPVGEA
                     MIGRVVNAIGQPIDGAGPIETTEYRPVESPAPGICDRQPVKEPLQTGIKAIDSMIPIG
                     RGQRELVIGDRQTGKTTIATDTILNQKGKDVICIYVAIGQKRSTVASLVAELQAGGAM
                     DYTIVVAATASETSPLQYIAPYSGCAMGEYFMNKGKHVLIIYDDLSKHAVAYRALSLL
                     IRRPPGREAYPGDVFYLHSRLLERAAKLSDEKGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLETELFHSGIMPAVNPGISVSRVGGNAQIKAMKKVAGTLKLVYSQYREL
                     QSFAQFGSDLDADTKARLAQGARIVEVLKQGRSTPVPVEKQVAILYAVVKGVLTDVAV
                     EDIQAYEEGLYTFLDSDAQGVAAMEGIRTTGKLEGETEEKLKAALADYTDRFLKTR"
     gene            complement(26654..27193)
                     /gene="atpH"
                     /locus_tag="ACZPCL_01795"
     CDS             complement(26654..27193)
                     /gene="atpH"
                     /locus_tag="ACZPCL_01795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR1161257.1"
                     /translation="MTKTAKIYGDSLYDLAAEEKLTEEILPQMEQVAVLFKENPDYLT
                     LLSEPSIPKTERVGLLDKAFEGQLSLYLLNFLKLLCESGMLREYRDSCRQFKARYNED
                     HNIAEAVVTTAVALTEAQAQALKERLEKISGKTVLLTQKTDSTVLGGIRVELEGKQLD
                     GTVQERLSSLKKKVTEIIV"
     gene            complement(27190..27696)
                     /gene="atpF"
                     /locus_tag="ACZPCL_01800"
     CDS             complement(27190..27696)
                     /gene="atpF"
                     /locus_tag="ACZPCL_01800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR1161258.1"
                     /translation="MRETQDLVTIIPWTFIAQICNLFIQCWLIKKFLFKPVCAIIEKR
                     RALADSQIQDAKKAKEEADAMKADYEAEMAKAKETANSILQNAQKDAAARSEAMIQEA
                     QTQAAGIKAKAEADIAQEKKKAVNDIKNEIGGIAMDIAGKVIEREISEEDHKKLIDEF
                     IENVGEAS"
     gene            complement(27793..28074)
                     /gene="atpE"
                     /locus_tag="ACZPCL_01805"
     CDS             complement(27793..28074)
                     /gene="atpE"
                     /locus_tag="ACZPCL_01805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009428481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR1161259.1"
                     /translation="MTDFQLLAKGIALAGCAIGAGAALIAGIGPGIGEGNAVAKALEA
                     IGRQPECKGDVTSTMLLGCAIAETTGIYGFVTGLLLIFVAPGTFMNYLS"
     gene            complement(28140..28901)
                     /locus_tag="ACZPCL_01810"
     CDS             complement(28140..28901)
                     /locus_tag="ACZPCL_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009216294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR1161260.1"
                     /translation="MNFNISGAAVYFRIPTGIPILGDFLISETLVVSWIVMILITGLC
                     IWLTRDLKVENISKRQAVAEMLVEQAQKFVRNNTGGTKFDNLIPFVAALFTTSVVSNL
                     ISLTGLRSPTADLSTEAAWAVVVFIMITSNKIKAGGILGYLKGFTTPIPVMTPFNVLS
                     ELATPVSMACRHFGNILSGVVINGLIYAALAVATSALLGWLPGALGQVLGSIPFLDVG
                     VPAILSVYFDWFSGFMQAFIFSMLTIMYIANAAEG"
     gene            complement(28924..29346)
                     /locus_tag="ACZPCL_01815"
     CDS             complement(28924..29346)
                     /locus_tag="ACZPCL_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HIR1161261.1"
                     /translation="MKIQPAVKKETGHIACWSGAGTILMIVAFAVCHKLFPDAVPFDY
                     KVVLGGVIGAAVSVGNFFIMGLTVQKIASGEQSDEQAYKSMKISYRYRSMAQLLWIVL
                     AMVLPFVNAAAGIIPLFFPSLSIKLINILGIIKAKPNL"
BASE COUNT         7054 a         7502 c         5989 g         8838 t
ORIGIN      
        1 aacggttatt tattataacc ggttgttttt catttgtcaa caactttttt aatttatttt
       61 tttgtttctt tcgaaacatt tgctgctctc tcaagcgcga aggctataat agcaaatcac
      121 aaagcgtttg tcaacaactt ttttaataat tttaaatttt tttaaaagta taaaaagttt
      181 aaacaataat aaaaagtcaa caataaaaat gccccgaatt ttaaggactt ttcagggcat
      241 ttcatacata tgatcctcta actcattaat acggcttcca gcctactcaa aaaccgtcct
      301 ctggcacctt cctggttttt aaggctcttt aacttatccg tgctcttcgc cgggatccat
      361 gctttattgg tattataata aaaattcagc tgcttccagt acttttccgg aaacaggaac
      421 atatagtaaa ggcattttcg ctctttctta tccatagggc gaacccgttc ataggagtct
      481 aacatggata tgccaagatc acagtcccag ctgtgttttt ccattacttt tcgcataagc
      541 cggtacaggt cttctgcctg gactcccagg tgcatgcggt tatattcaat aatcgctgtg
      601 taaccatttc ccataagtac atgatgctgg tccaggtcac cgtgacagag atagccctga
      661 ttttccccgg atccttccgg cgccagttcc gcggcttcca gcctgtcagt gatcccgttt
      721 tctggaaaca ggctgttcat ctgccttacg gcttcttttg cctggtcaaa gaaatgatca
      781 aagctgtcta ttacacaaag ttcaaattct gtttttcccc gtttgccccg gataaaattc
      841 ctggcacgct tcatttccct tacatggcgt tcctgctctt tttccaggct ttctatacag
      901 atcgacccca tattccattc ttctcttaca gggatctgac gcagctggcc atggagcatg
      961 gcaagccggg aaatcccctg acggatctca tagctgtccc tgatattaca ttccctgtca
     1021 gaaaaccatt ctttcataat ataacctgtt ccatccccgg ctttgcttaa aagagctcct
     1081 tcctggttac gtacatactg atctgcccgg agacttcccc tggtatccag gcattccaat
     1141 acctgatctt caaattccag tctgcgcaca gtccccttat attcccgcaa aagcttcagt
     1201 cccttatcgg tttcacagat ccaggcgtcc ctgccttttc gcacagacag gatctccact
     1261 tcatattgtt ctaaaacctc ctggtatctt tcattcattt taaccctccc accaagttca
     1321 aacctctgac cacatttaca gttttccatg atcttcactt aaactgtctt ttactgcttt
     1381 tacagactat gtagacaaag gcctgattat gcgggagttt ttgcccttaa atgtttacag
     1441 acacaaaaag tccccctgca aacgcaggag gacaattggt tcatttattt ctgttcccag
     1501 ccatctttcc acttcatgtg gtgaagctgg ccttctaatg ccattttttc aaaatcatta
     1561 tggcgcaggg cctcgtaaag caccactgcc acggaatttg acaggtttaa ggaacggatc
     1621 tcgccccaca tagggatgcg gacgcagtga tcttcatttt ccactaagat ctcttccggg
     1681 ataccggcgc tttcttttcc aaacataaga tagcagtccg gctcgtagtc tacttctgag
     1741 tagactttat gtgcctttgt ggtagcaaaa tacatttttc ccagggcatc cgggttcttc
     1801 tccagaaaat cctggtaatc gctgtataca tgtacatcta atttatccca gtaatccaga
     1861 ccggctcttt ttaaagcttt ttcatttaac ttaaagccca gaggctcgat caggtggagt
     1921 ctggtgttgg tagccacaca ggtacggccg atatttcctg tattagaggg catttccggc
     1981 tctaaaagta caacattcat cataatctta ttctgccttt ccatcatggc gctttacaaa
     2041 gcgctcaata cggcttaacg cctctttcag gctctttaag gagtacgcat aggaaatacg
     2101 tacaaagcct tctccgcagg cgccaaaggc cgttccagga actacagcta ccttttcttc
     2161 catcagaagc ttggtagcaa attcctcaga agtcatacca aaacggctga tatttgggaa
     2221 tgtatagaat gcgcctaatg gctcaaagca gtcgattccc atttcacgga atgcatttaa
     2281 taaaaatctg cggcgctggt cgtaggactc acgcatcatc tctacatccg gatcaccatt
     2341 tttcagtgcg gaaactgctg catactggct ggtggtaggc gcgcacataa tagcatactg
     2401 gtggatcttt aacatctgct ttaagatcag gtctggcgct gccgcatatc caagacgcca
     2461 gccggtcata gcataggact tggaaaaacc gttgatcagg acggtacgct ctttcatgcc
     2521 tggaaatgct gcaatagtag cgtgttttct ctggtaagtc aattcagaat agatctcgtc
     2581 tgttactaca aacaggtctt tttcaatgac gatctttgcg atctcttcca gttcatgttt
     2641 ttccataata gcgccggttg ggttattagg gaatggaagg accaggatct tagtcttgtc
     2701 agtgatcttt tccagcagct tctcaggagt cagcttaaac tggtcttttt cctccaattc
     2761 aatgatcacc ggaacaccat ctgccagagt aacacatgga agataggaaa cataacttgg
     2821 ctgtgggatc agtacttcat ctcccggatt taacatagca cgcatggcaa cgtcgatggc
     2881 ttcacttccg cccactgtta ccataatttc tgtatccgga tcatagctta cttcaaaacg
     2941 acgctttaag taattacaga tctcatcctt taactgtttt aaacctgcat tggaagtata
     3001 gaaggtacgg cctttttcaa gggagtagat gccttcttcc ctgatatgcc agggagtgtc
     3061 aaaatccggc tcgcctacac ctaaggaaat agcgccttcc atttcattta caatatcaaa
     3121 aaatttacgg atcccggagg gcgggatact tacgattttg tcagataagg ggtttctcat
     3181 ggtgtgatca gcatcctttc atcctggacc ggctcactga taatggtgcc gtggtcctta
     3241 tattttttca gtacaaagtg ggttgctgta cttaatacag actccatagg agctaatttg
     3301 tcagatacaa actgtgctac ctggcgcatg gtctttcctt cgataaatac ggtaaagtca
     3361 aaagatccgc tcatcagata tacagcgttt acttcattat actggtagat gcgctctgcg
     3421 atcttgtcaa agcccatacc tctctgagga gttactttta cttcgatcag ggctactact
     3481 ttttcttcac tggtgttatc ccaattaatg agagtgtggt atccgcagat aatgtgctct
     3541 ttttccatct cggcgatctc gttggccact gcgatctcac tttcaccaag taataccgcc
     3601 aggtctttga tatcgatcct gctgttcttt tcgatcaccg ccagaatttt ttccctcatg
     3661 ataacttcct ccaatattaa aatcacaata ttaaaatcat atattcttat atggtattgc
     3721 cgtcaatacg gctcagttca tcggctcttg gccgctctaa atatccggct gtttcctctc
     3781 cgtggcgtat aacacgggca attccctttt ctgcgctgcc gatcactgct gcagggaccc
     3841 cctgtgcgct gaggcttcgc accatctggc cgcctctgtc acaggctaag atcacgcagt
     3901 tttcactgaa aagacggtaa ggattgacct caaagatctc acatacttcc actactgcct
     3961 gtttcatagg gatcatgcgc agatcaatgt ctacgcctac gttataaata ccggataaat
     4021 tccatactgc cgtgagaata ccgccttctc ctgcgtattc ccaggctgta accggacaat
     4081 ttttctgctt taattcattt tcaagccact cttttacgtt gtagctgtct ttttcttcca
     4141 gacatctaag gaaagaaggc gcaaaatgcc cttccagttt ttcccttttt ttctggaaga
     4201 tctgtctggt tccggcaaga ccggcaaaac cagccactac cagatcctga cctggcttta
     4261 taaagccatc tcttttttca aatccgcccg gaccctgtcc tgctgttctc atggattttc
     4321 ctcctgtgcg ggactggccg ggctttccgc accagcatta tcccccgcgg gctgcgctgc
     4381 agtggtggga acaactcccg gtccgccatt ttctcctgta actgctttat gttccgcctc
     4441 tgtctgagcc ggttctgtct gggcagtttc aggtgtatga ttttctgctg tttcaggtgt
     4501 agtggcaggt gctgtctgct ctggtaccgg tgcaggtgcc gcatgggccg gtcctgcacg
     4561 gtaaatggct ttggaagcat tgtaagtatc ctcatttaaa agggtacgct cctgttccac
     4621 tccgtctacg gtcactacct tccagagtct ggacttatat ccggtatggg cagactgtac
     4681 tttcaccttt gcacctggtg caaggctctt atccgtgatc atctgcggct ctcctggatt
     4741 agtcgtgcct atggtctctg aaacatattc tacctgtctg ttagaaggtc tggtctcttt
     4801 tccgtagaga gtaaaagtca gcttctttcc agatgtatat ccttcgatat atacaggtgt
     4861 gctgtaatta ttcttaattt taatatcttt ataagtgcct gcaatggctg catccataga
     4921 aggctttaca taagttacga tcatggaatg gttctgtctc tgaacgatct ccatttccgc
     4981 ctgcagcgcc gcatcataca gtgtagtagc gatctggcag acaccaccgc cgatactgtc
     5041 tactacctga ccattttcat aggctgccgc tgtcttgtag ccattttcaa gagtaaacgg
     5101 ctgaaggcat tcgtaaccag agatcacctc tcccggcata agcacacggc cgttgatctt
     5161 tcccgcacca actgcaaggt tggtggaacg tgccgcgccg ctgctggaaa agtcagtggt
     5221 acaggttcct aaaacatctt ctatggtctt aagatcatcg gaagtaatag ctggctgctc
     5281 ttctatgatc actgcctgga cagagatctc accattagag tcttttaatc cctcaaaagc
     5341 tgtatctaaa gcggaagccg tggcatctgc atccacgact tttcctaaaa cagactctgt
     5401 gatcacaaat gcgccgtttt ctctgcggat agacgcatcc tgaggctgtg ccagtactcc
     5461 gtcgcaatgg gtatctacaa aatctcttat cttatccgga tcggctgctg tcttgatgga
     5521 aacatccact ggtgcctttt ccagatcctt tttgttcata tactgacgga tcaggcttcc
     5581 gcctacatag ctttttagct ctttttcagc ttcatcctga ttgctccagt gaaagcccag
     5641 ctctccggct gtagtctctg cctttttgtc ctcaatggtt aaggcaacgg atatacccgc
     5701 ctgatctttc actttttctt ctgctgtatg cagggcatct tcccaggttt ttccagccag
     5761 actttccccg tctgcgctga taccttcagg aaataccggt gccgcccatg cactcatggg
     5821 aagcacccat aaagccccgg cagccagtgc tgcaaaaagg cctgctttta atgtcctgtt
     5881 tcccataaat tctcctaatc ttttaccgtt ttaatcagtg ctgcatttaa gcagcattta
     5941 attaatacat cagtgcgctc agtgccattg gtacgatcat ggcaagtacc agaattacaa
     6001 cgataacagt agaaatgatc ttctttactt tagggtctct catatttaac attttatcac
     6061 ctcatttatt ctgtggcttc acccgtccgt actggaggat gatcttgtcg atcagtctgg
     6121 aagcgtcgct ccggcttata tcgtctaact gcacagttat gtctattcta tgacatttta
     6181 ataaatcctg caagtattct ttttgccttt tcgttgccgg ttgctgccgc ttcatccggc
     6241 agtttaaagg ggttgtcaca aagagttccg gctgttcttc tccatgaagg gcaaccagct
     6301 tctgatacag gaaatgggcg gataatgcat cggcctctgc ccggtgcgcc aggggctgtt
     6361 cgatcttata ataggtacag gcatttccca gatttttctt ttcctgctcc ggcatcagtt
     6421 tccggcacaa tttcagtgta tccactgcct cacattcaaa ttccattttc tggttcactg
     6481 cagcctgctt taagaaactg tagtcaaagc ggatgttgtg gcctaaaaga ggcaggtttc
     6541 cgataaattc cagcacttcc cctataatat cctcgattac aggagcatct tttaacatgt
     6601 catcggtaat gcctgttaat tccacgaccc tttctgtcag cggccgtttt ggatttacca
     6661 gagttacgaa acggcctgtg atctgtccat gctctacttt tagagctgcg atctccgtta
     6721 ttttttcttt tttcggctcc agacctgtgg tttctaagtc cagggcaatg tatgagtcag
     6781 tcatttcgac gtattccttt ctattatgtc agaagttttg tgattttaca ttttcctgtt
     6841 taatctattt tttctgtttc tttgaaaact gccggcactt cacaggcggg acaccactgt
     6901 tttaaaaagc agttcccaca gtccggtttc ctggctgtac agatcgtccg tcccagtgtg
     6961 atgatatgga tattccagag gatccagtga tcttctggca gcaccttcat aaggtccgct
     7021 tcgatctttt ccggctctgt ttcttttgta aggcccaact tcctggaaat acgctttaca
     7081 tgggtatcta ctacaatact tggcacatgg taaatatttc cccggatcac gttggctgtt
     7141 ttccggccta cacctgccag tgtaagaaga tcctccagtc tttccggcac aatgccccca
     7201 tattcttcca ccagccgttt cgcacactgg ataatgtttt tggctttcat atgataaaat
     7261 ccaatggaat ggatgtcctg ttccagttct tttagatcag ccgctgcaaa atcttccagg
     7321 gtcggatatt tcttaaaaag atcggctgtt acaatattca ctcttgcatc tgtacactgg
     7381 gcgcttaaga tcaccgctat aagcagctgc catggggttt cgtagtttaa ataacagatg
     7441 aaatcagtgc cgtattcttt atctaagact gataatatat gagagatccg ttcttttaat
     7501 ttttctgtca ttttgatcat atttgtttct atccctttat cctaatttca tttcaagttt
     7561 gcttcacatc ttaatttgca ttttacaatt caatcgcctg tgtctataat aaaggaaaat
     7621 gtggaaaagg taaatgtttg agaaggtaaa agaggataaa acatgcagtc aaagaaagat
     7681 ttataccaga tcggcgaagt ggcccgtctg tttcatttaa gtatcagcct tctgcgccac
     7741 tatgacaaga cggggctggt aacgccggag tatacggacc cggacaccgg ataccggtat
     7801 tacagcatcc gtcagtttga atgtttaaat acaatacgct acctgagggt cttggacatg
     7861 ccactggaag aaatcgggga ttttctccac aacagggata ccgggcggat ccaggagctt
     7921 ttgtcgaagc agctggtgca ggtgcataaa aagcagcagg agctggctgt gatggaacat
     7981 cagataaaaa accgtttaaa ccagatccag ggggctcttt ccgcccatcc gggggagatt
     8041 tttttagaaa agaagccgcc tcttaagctg gcctgcatga aaaagcagct gaatccggaa
     8101 aattatctgg atctggagta ttctatccgg gaaatggaac agggaaacag ccccgcttct
     8161 attttccttg gaaaagtagg agtagggctg ttttatgagt ctttaaaggc acatcgcttt
     8221 aaaccttatg attttgtttt tatcattctg gaagaggatg atcctatcaa agggcaggtc
     8281 attcacatcc cggaaagcac ctgggccatt gtccgttttc atggaagcca tgaacaggcg
     8341 cctggatttt atgaaaagct tctggcctat tttaaggacg agggatttgc ccctgaaagc
     8401 tttgcactgg aaatgaccct catcgactac ggactcaccc atgaccagtc ccagtttatc
     8461 acagagatac aaattccctt aaacccggac tgtccgggca ttatagctta atggatcccg
     8521 ctttttgtag tcaaacagca gataggttcc atctcttaat ggctctaaat gggccatttt
     8581 ggccatctgt ctggagtcgt agccttcata gccagacaga tgctccggag ttttctcttc
     8641 tttctggaaa aattcacgga caaagtcttt ggaactgctt aaatctcttg caaaggttgg
     8701 gcggctcttt acattttcac gaagatacag gtcggtcatt agcaggtcac ggtataattc
     8761 taatttttca gaaatgttaa cattttctgc cattgcctga gtttcacatt ttccattttt
     8821 ttccaatctc tcctgtatta tcttccagag gatctcatat ctggcaatgc ggctgtggct
     8881 gataccaaac aaatgatttt tgttgtaata acctgccata aaggaaaaca tttcataagg
     8941 agtatcaaat tccttttcca gttcttttaa agtacaggta aactgacggc tgttgtaatg
     9001 aacttccgtc atttcttcga tatcttttaa ttccagcacg tccccatagg aaagccattt
     9061 ggtacacagc acctcataag gcggcgcggc actgtaaagc aggtcacagt ccgggatcag
     9121 ttcttccata taagagcctt ttaatacctt taaaaagcct aactgcagct gctccggctc
     9181 catttcatat acatcattaa aggacttttt aaagctttcc agatcttccc atggaagtcc
     9241 tgcgatcaga tccagatgct ggtggatgtt gtgccagctg ttgatggttt ctgtgatctc
     9301 acgtatctct tcgatgtcgg ttttgcgccg gatggcttcc aatgttttct cattggtaga
     9361 ctgaacgccg atctccagct gcaccagtcc cgggcgcatg gtggaaagca gatccagctc
     9421 gtccttatct aacaaatccg ctgcaatctc aaaatggaaa ttggtaacgc cgttatcatg
     9481 gtccctgata tactgccaga tagccattgc ataggacttt ttacagttaa aggttctgtc
     9541 tacgaatttt acctggggca ctttcgcctg aaggaaccac tccagttccg gcagtaccag
     9601 atccaggctg cgaaaacgca gtttcttatc aatggaagac agacagtagg cacaggaaaa
     9661 aggacatcct ctgctgcttt cgtagtagat aatgcggtgt tccagttctt tcatatccat
     9721 aaaagcatag ggaaatggga tctggctcat gtccattacc tggcgaaaac ctgtatttgt
     9781 aaatgtgcca tcagcatttc gcaccataag accgggaacc tgttttaaat cttctccttc
     9841 aaggagtgcc tttagaaaat gggcgaaggt gatctctcct tcccccatca taacaaggtc
     9901 cactgccggt tcttctttca aaaaggcttc cccatgatag gaaacttccg ggcctcctgc
     9961 ccatattttc acatctggaa gcaccttttt tatgtcagcc agtatcattt tcacatagga
    10021 aatgttccag atataacagg aaaatccaat gacatccggc ttacgcttat aaatatcctg
    10081 aaggatctgc tccatctggt ggttaatggt gtattctgcc agagagatct ctgcctgctc
    10141 tgccagtccc cattctttta acatcttttc cccataagtc ttcaggctgt agatacccag
    10201 attggagtgg atatattttg cattgatcgc ggctaaaaga accttcattt tacacctcga
    10261 tctcgatctt gcggcctgta ggctgatact gataataacg gactcctgct gcatttaaca
    10321 tgcgcttgga tgcttttaca gctggtgtat gagcatattt atccccaccg tagataatgg
    10381 tcttaatacc cgcctggata atggccttgg cgcactcatt acacgggaaa agagttacat
    10441 acaatttgct cccttccaga cttcctcctc tgtaatttaa aatggcattt aactcactgt
    10501 gggtggaata gaaatatttt gcattgtacg gatcgtccag ttcatgttcc ttgccccatg
    10561 gaaattcatc atcggagcag ccctttggga agccattgta gcccatggaa aggatcttgt
    10621 tgtcctggct tacgatacat gcccccacct gggtgcttgg gtctttggaa cgtctgccgg
    10681 aaagagtagc aactcccata aaatattcat cccatgtaat ataatcaaca cgtttatctg
    10741 acattgtatt tctccttctt gcatcaattt caactatgat accggattgc tgcgtgcttc
    10801 gcactctcgc aattcttaaa aatatttata attatcatat aatagacgac cttaaaatgc
    10861 aagaaggagc agccggatca gctgctcctg tgtatagaat tactgctatt cctgcattac
    10921 actgcaggta atgtaacgac cgctatttat gccttggcca cattcttggt cacgatcacg
    10981 tctgcatcca gacctactat tcccttgatc acaacggtac ctgctttcac aggcgctttc
    11041 aggcggcatt ttttgatctc atccattgca tcgaaaatac gcgccttggg gatcggggca
    11101 gtaaggcgta cactggcaag aggcagttct cctccttcta ccagtacgga ggttgcaatg
    11161 tttctcttcg gatctgtcag ctcctgtttt acatactctg ctcctctttt gcaggtggcg
    11221 ccctctaatg aacggatcac tacggactga tctgcctgta tctcataatc tgcgcttatt
    11281 tcacagccat ttggacaaat gatgcatgta aattctcttg tcattctacc ttcacctcca
    11341 gatctccttt attttccagt ttttctgctt ttaaatctat atggatcatc tctgccggaa
    11401 gggctttttt catctttttg cgtgcaattt cttttccatt ctggcttaca actaaagcac
    11461 aatccttaaa tggccggttc acacgcaggg acagacaaca gtcttctgtt ccggagatcc
    11521 tctgtggaac cgtatgattg atatttttat cagtctttac ctggatctca catgcaggca
    11581 gttttccctc tttgatatac cgggctgcgg agtctgccag cttttctgct tccatggata
    11641 caaaatccac cagatcgtgc acatgaagga catttcctgc tgcgaaaatt cccggccggt
    11701 ccgtctggaa atattcatct actgtagcgc cttttgtcct tgcatccagt gttactcctg
    11761 catccaggga aagctcattt tccgggatca gaccaacaga taagatcagt gtatcacagc
    11821 tgtattcctc ttccgttccc gggattggcc tcatatgttc atctactttt gaaacagtta
    11881 cgccggtcag acgcttatct ccgtggatct ctgtcactgt atggcttaaa tacaatggga
    11941 tattgtaatc atttaagcac tgctcaatat tccttggaag gccgcttgga tatggctgga
    12001 tctcaaaaac tgcctttaca tgggcacctt ccaaggtcag tctgcgggcc atgatcattc
    12061 caatatcacc ggaacctaag atcaccactt cttttgcagg catacggtta taaaggttca
    12121 tatatgcctg ggcaactcct gctgtaaaca cgccagttgg ccgctctccc ggtatgccaa
    12181 gtgcccctct ggtgcgctcc cgacatccca ttgcaaggat cacagctttg gctttccact
    12241 gtttcagacc atcaggagtg gctactgtga tcactttatc ctctgaaatc tccagaacag
    12301 ttgcatcagt aagataacgg atcttctttt cgtttgccag gtcaatgaat ttctgggcat
    12361 attccggtcc gctgagtgta gttttaaaac gggccagtcc aaaaccatca tggatacact
    12421 gacgtaaaat gccgcctaac tgtttttcac gttccaggat cagaatatct gtgatcccgt
    12481 tttcatacag gcgtacagcc gctgccagac ctgccgggcc gccgcctacg atcactgcat
    12541 ctgcctgttc tatttcccac atttctgttt tatgttccat gttcacagtc acacctcccg
    12601 tacctttcca aataagacat tagaaccttc ccgctcatac tgcacttctg tctctttttt
    12661 gccaagttcc tgttcgatca gctgttcaat acgcatctgg cagtagcccc cctgacaccg
    12721 tcccatcatg gatcgggtcc tgtatttcac acctgtcata gtatctacgc ctaatggatt
    12781 gtgaattgcc tgaaggatct ctgcttttgt caccttttcg cagcggcaga tcacttcgcc
    12841 gtaatccggg ttctctttga tcagctctgc ctgctcttct ttggtgcact gggcgaaatg
    12901 gcggattcct tttctcaccg ggttgaaatc cggattttcc tctaatgttt ctctttcctt
    12961 catcagttca atggcgtatc tggcaatggg cactgctgct gtcagtcccg gtgactcgat
    13021 acctaccagg tttaatgcat gaggcgcgat ctcgtctttg atctcgatct taaaatcctg
    13081 gatcacaccc ttttcatcta cccattttgg aaggatacca gaataattgc ggatataatc
    13141 tgccttatgg atacatggcc acaggtcaga agcactttct gccagataat ccatattttt
    13201 ctgaggcaca ccgtaataag taaaatctgt taccatatct gcgttagggc cgatgatcac
    13261 atttccgtct gtggtatttg tcacatgaat tcccatatag gtgttgcttg gaaccggata
    13321 aacaggcata ggaagaagcg gtccggtgcg cttatccagg ataatataat ctcccttgga
    13381 accgatgatg cggtagccgg tgatccccag catatcagag atcttgccgc agccaaggcc
    13441 tgcgcagttt actacccagc gggtcttaaa gtttcccttg gttgtggtta tttcataagt
    13501 atcatcatct tctctccgga ttgcagtcac ttcacagcca aaatgatact ccacaccgtt
    13561 tgcatgggca ttttctgcca gggcaatggt atagccaaag ggatctacga taccgctgtt
    13621 atgggaaaac atggcaaatt ttcctactac tgcaggaacc agctcatgaa ggcgtttttc
    13681 atcgatcagc tccaggttag tagctccgtt gtcttttccc tgctccatgg tacgctttaa
    13741 ggtctccata tcttcctcgg tatttcctac cagcacctta ccgcagcgga taaaatggaa
    13801 atccagctct tttgccagat ctcccatcat cttatttcct tctacacaaa gttttgcctt
    13861 taagcttcct ctgtcatagg cgaaaccacc gtggacaaca ccggaatttc gaccgctggt
    13921 ctcattgcat acgtccagat ttttctccag cacaccgatc tttaaacggt accgggacat
    13981 ttctctggcc atggcacttc ccactacgcc accaccgatg atcagaacat catacatctc
    14041 cttcattttc ctgcctcctg tgttcagcat tttattttgc tttaaattca tcccatgaat
    14101 atattttata tttatcatat atgacaatat aagtcaatgt atttctgatt ttctttgtaa
    14161 atttatagga taaattcaat ctatagtcaa caaatggatc tctgatacgt tctcagtata
    14221 tattgcagca aaaaagcagc cattacccat ttttgagtaa ggctgcttct tgctgatatt
    14281 ttcgctgaat tttactctta aagataaatt tggcagatca cagatattat tctgtaacca
    14341 gagctgctgc tacatctttg ccctttgcac cgtctttgat gatgaacagt tcgttggtat
    14401 caaaaccagc gtccttcata acagcgatcg cggtctttgc acacttgtta ccgctgtagc
    14461 agagaatgta tacaggcttg gaagaatcca tggtctgtgc cagttcgtac attgccttct
    14521 gtgcatctgc gctctcaaaa tcttttaatc cgcactgcag ggatcccttt aaatgtccct
    14581 gtgcataggt gtcatcatca cgtacatcta agaactgaac gtcatcgctg cctactgctg
    14641 caactgcgtc tttgccatct acatactgcc agtcaatgtc ttcttctgta cggtttccgc
    14701 cgaaagttac ctctgctgca gcctcagact cttctgcctt tgcctctgct gctgtggttt
    14761 cagctgctgt agtctcagct gcagttgttg ctgctgtggt ctgtgtagat gcagaattgc
    14821 cacatccggt tgacataacg actgctgccg ctgctgctaa aaataaaact ctccactgtt
    14881 tcttcataat aaagttcctc cttgtgaata aaaaaatata tttatttata gtttaaaacc
    14941 ggttccagat gttctcgatc cggcttaaaa actcatcaga aagatatact tccagtttat
    15001 caaaggtcga ctggttgaat atttctttat ctggctgctg atactgtacc catgccatag
    15061 cgcaccagat gatcccacgc aggcaggtga ccggaatata aatataggtc cgctccttta
    15121 agcctgtagt atcaaaacgg ccgtttactt tttctatata tgtatctaaa aaggcatcta
    15181 tccggtcctg gtccaggatc acatctgttt tccagaaggt agtcgtaggc gccagaaaat
    15241 gtcccagatc ctgggcggga tctccatata atggtttttc ccagtctacc agatatgctt
    15301 ttttttcatt gtcttttatg cagtcacccg ttttttcacc aacactattc tcattgatcc
    15361 ttctgccatc aacattcctg tcattaacaa gaaaattggt agaattcaac tctgtgttga
    15421 tacagcagtg atagggaata tccgtttcta aactttctac tgccttccag ccctggtcta
    15481 acaggctgcg tatcctgcgt tttttctgct catcccccag ttctgactcc atataggtct
    15541 ttaccatggc ttcacactct tccagtatgg cttttaaagg ttcttttggc tggatcagcg
    15601 aatgggtttc cggcattttt acactgtgta tatctgccag acagcctgcg gcagaaaaca
    15661 gttctgtttt ataatccaga ctatgtcctg gcagaaattc catgaccata acaccatagg
    15721 gaagattttt gcagcttccg tctgcataaa tagcttttgg cgtacgcccg gagttttcca
    15781 acagcttcaa tgccctgtat tcatatagga tctggtcttt taaatgcatc tggctgccaa
    15841 aattgatccg cagcaccagc ttctgtcccg ttatgggatg agtaaaggta taattgcggt
    15901 tgtattctcc ctgggcaagc atggtatagg tttctgtcac ttgtccagac agccccaggc
    15961 tttcccggta ctcttttgtt tgaatgtatt cttttaatcc tttcgtccgt tccatgacct
    16021 gcggctcacc taccttacat cccggtataa ccggtcgatc ttttctattg gaacatgatc
    16081 ccggtacatt tttcttaacc ggttcatttt ccacattaat tttaatttgg gtatcgtacc
    16141 ttttgggaag cggcgatccg atgtatagat ccgctttttc gccattccca gccggactcc
    16201 catttctttt aatgtcaatg aaaactggta atcttccata accgggatca ccggaaattt
    16261 tcctgcctgt aaaaacagct tcctgtcaat aaagatcccc tgatccccaa acatcacctt
    16321 ccgatccttg atcctgtgat tggaaatgac ccggcaggta aacataaaga aattcccgga
    16381 atgaaaggcg atcccaaaac agcccgcgct gtggtctttc atcacccggc ggatctcttc
    16441 taatggtctt gggggcagtt cactgtcaca gtgaaggaaa aacaggatat ctccatggct
    16501 ttcctctgct cccagattca tctgattggc ccgccctttt tcagaacgga gcagtttaaa
    16561 atccggtccg atcatttccg gcgtcccgtc tgtgcttcct ccgtctacaa aaaggatctc
    16621 acagcgggag cggtagggat acagctgctt tttcatagct tctatggtct ttgcctcgtt
    16681 atagaccggg atgatcacag atatttttac atttgctgcc agataccggc ctgtatggct
    16741 gttctgaagt ctcctgtcct gacgcatccg ctgtctgtaa cattgaaggt cctccggcgt
    16801 atccatatcc tgatgtttcg ttgtaaatcc aacatttatt cctgcatctg aaagttcttt
    16861 taaagtatct tcaaacacat ggctgtgacc atattgcttt aagccaaagg cctcaggtat
    16921 gggccgcttc ataccaacca gataatagcc gccgtctgcc gttcttccaa aaacagcgtc
    16981 tttttctttc aatacctgaa aagccttttc cagatccggg ctgctgactt ctggaatatc
    17041 tgtccccata agaaggcaaa cgtcatatcc cagccccaga acatattcca gactgtgata
    17101 catcctctgc cccagggact ctcctgcctg tggaaaatat tctttctgct tcccaaggat
    17161 atccttaagt tccttcacat ccccgtcttc aggtgcatag cacacaaaac aatctgcttt
    17221 tacttttctg cactgttcac tgatatcttt cagaaaacag gtatgaagct ttgcgcactg
    17281 ctttggtgaa aaatagggca tcaaccgggt ttttgtcttt cccggtaccg gaatccttgt
    17341 aaaaaggatc agcgcctgtt tcatttacac cagctcctct aaaatagaca gcacatccag
    17401 aacaatctgc ggacagagat caaacataag gcctttttcc agcaccttat ccatttcccc
    17461 cggttttgaa atatcatgtc ccaggatctc acggcacata tcggaaggat atttttccag
    17521 aaaacgcttc ttaaattcag ccacttttcc tgccatgatg cctttctgat caccatcatt
    17581 ttcctcgctg tgtccaaatg ccattccaag gaccattaac gcgccggtca cagcgccgca
    17641 ggtctccccg cacatcattc cgcctccgaa acaggctgac attttccttg cctgttcttt
    17701 ggtcattccc attttttctg cacatgcgcc ggtcactacc tgagaacagt caataccggc
    17761 cataaacaac tgacagactt cttcttttgt catgttctta tcctcttttc tttttcatat
    17821 gttccatatg gatgctgcgc atcataaaga tcagcgcgga cagggcaaaa atgatgagaa
    17881 gccctgtaac aaataactta gctccgccgg taagcatgcc gcctacatag gaataaacaa
    17941 tggttgcagg cagctgtccg atcccagtgg caataaagaa ggaggaaaag gacatagatg
    18001 taagtcctgc tgcgtagctt acaatatcaa aagatacaaa aggcagcaga cggcagatca
    18061 ggatcgtatt ttttccgtat ttttcaaaaa atgtatcgat ctgtgccaga ccggaacggc
    18121 tggtcagctt ttcagccaca tcacgaccta agattcttgc aatgtagaaa cagattgctg
    18181 cgcctgccat agcactggac caggataaaa tagctccctt ccaccagcca aataagtttg
    18241 cattggcaaa ggtaataaga aatgcaggca atggcgcgat aatggactgc aggatcatta
    18301 acagaaaaga taatgccgct gcataggctc cataggacgc tacaaaatcg cgaagcacgg
    18361 taaaatcacc ggttgcaaac atggcaaaaa cacaattcat gccctggtgt acggacggta
    18421 ctgccatgta tacagctata gctgctacca cgcaaacgat caggatcacc ttttcgatcc
    18481 atttattttt attcttcttc atactttctc cctcttctat cttcctgcac gaacccggtt
    18541 ccacagagaa tgccttacat ccggcacttc ttctcttcca aggatctgta ccagcagatg
    18601 ttttatattt tttccgccgg ctcttgtgag atttccggca caggaggcac aatacacgct
    18661 tgtattctgt atactgcctg ctgattttgc catctgagct gcaagttccg gttctttcaa
    18721 tgctgcgcag cctccaagac cacagcagtt tgcacttgaa aggatcttcg gttctgctgt
    18781 cagaaatggc cggatctgtt ttaaaagttc tctcttttca cggtccggac atggaaggaa
    18841 aatattctgc tcttccatga tcctgtttcc cagccccagt tcctgtaatt tttcataaat
    18901 gctgatcacc ggaacagaaa gtttgtcttt caaaaaagca taacagtttg gacagaccat
    18961 aaccacttct cttacccccg cttccaggag ctttttattt aagcgttcca ggatcactgt
    19021 ttctttttct tccagtccca gctccgctat cgacttccca cagcagtcaa atacagaaaa
    19081 tgcatctgct ttttctgcca gcagtttccc cagataacgg gtagtctccg gataaaaaga
    19141 aggaaagtta cagccgaaaa acaatgctgt ttttcccgtt cctgtgtatc tgcggaattt
    19201 ataatcttct ttctcccaca ggagcatccc ataacccttt tcaggtatcc tgccgcctgc
    19261 ttcttttacc cggtgtcttc ggatttggag tactatttcc cgtccatcaa tcccctgggg
    19321 acagactttg gaacattttc cacagaggaa acagtgatat gccatcttgg aaagtttttc
    19381 tgtatctccg atggttaggt catatttctt taaaaactca cagttttctg tgcatacgcc
    19441 gcagtgggta cagcctgtga ccgactcttt catcacatct catctccatt tttcagatcc
    19501 gttttttcct gttctgcaag gacttttttc tttaacagga cacctgctgt tgttactgcc
    19561 accgctaata tagcggcccc aaggaaacag gcttttctta tacttggatc tgccagacct
    19621 gctgttccaa atgtatacat ggcagttccc ggaagcataa aaagaaaagt tccaaccgca
    19681 taggttttaa gtggcatgtc tgttacccca taggcaaagt tttgcagatt atagggaaac
    19741 aaaggaacca gccgggtgat catcaggatc cagatctgtt ttttccctgt ttcatcaaac
    19801 agtaagcggc gcagatatgg attttttctg gcagctgccc ctactgtatc tttcagaaaa
    19861 tatctgccta agagaaaagc tgcaactgct cccaaagtag ctgcaactgt acagcagatc
    19921 gtccccaaca ccggaccaaa cagcaatccc gcaaggattg caaaactgat ccccggcaaa
    19981 gcgaaagcta cacttcccac tgcggtaacc accagatata tgaaaatggc agcaggcaga
    20041 ttttctactg ttatatgctg taatgcctgg gtaagatccc catttaacag ccgcccggac
    20101 cagccagaat attttccaat caggaacagt aagatcatcc ctactgcaaa tacatatatt
    20161 tttcttttac ctgctttttg tttctgcact gtatttctcc tgtatacaac gtacaactat
    20221 tttaccatta attcccatta atgtcctatt gatatatttg gtgaatatat gctatttatt
    20281 gatattttct ataaatatat ttataatcag acaacaaaaa acgcgccatt ggcgcgcttt
    20341 ttctatacat ggtcatctct tattcttttc tgtaccggtt gtggttatac atcaaatgaa
    20401 aatacatttc ctgtcttgca tcttctcccc gtccatcacg gatggcttca aaaatgcgcc
    20461 tgtgggacaa taaagtctgc tcatattctg tttcttccac agagtccgca aaaacacgta
    20521 ctccctctgt gatcaccggg atcagattgg tcataacacg gttgtgggaa caggttgcga
    20581 tctgggcatg gaactgggag tctttttcgg aataatcctt tttatcccgg atcagtcctt
    20641 ccagttcttt taaaatgtat tccagttctt tgatttcttc ttctgaacca ttctgggctg
    20701 caagcgctgc aagtgccggt tccaggatca gtcttgcctg gagcagatct tcggtcagct
    20761 ggcgtctgtc atccatcata aagaagccta acggatcatc tgccaccccg tttttgtcgg
    20821 aaataaaggt tcctgagccc tgacggatgg taacgatatt ccgggacata aggatacgca
    20881 gggcctcccg cactgtgttt cgcccgaccc ccagtgtttc caccagttcc gcctctgtag
    20941 gtagtttatc tcccgctccg taacctttct cctggatcat ttccaaaagg ttctgggctg
    21001 ttttttctgc taaggtctgt ttttctgtat atttcatttc catagatcct gtttttcttt
    21061 cttctcagac tttatagatc tggtatccgg ctgctttgga cagacggttc ataatggcct
    21121 gtcctaaatg atcctgactg aagctttcgg aataaatata gtctactttt aaatcatcaa
    21181 attcacgaag gagggcatac aggttatggg ctacagatgc ctggctcttt ctggcaccga
    21241 tgctgcgtac ctccccctcc ggataatggg atcttgactc atcggtacag atgatgccta
    21301 ctttgtatcc tgcatctatt ttttcttttg ccagtttcca cacagtttcc accatagcgt
    21361 ttacctgctc ctgtgtcatg gcaacggctt cccctgtttt tctggcttct gccacatttg
    21421 caggttccac cagagtcaga tctgcctttg gagcataatg tttgtacttc ataccaggtg
    21481 cctttggacg tacaccggct gccatcggtc ctaagatagc cgggtcaatg gtcacttccc
    21541 ctaaaacatc ttttaacatt tccatggtaa tagcacctgg acgtaaaact gcaggaacat
    21601 caccggagac atctacaatc gtagactcca caccgatccc aacaggacca ccgtcaatga
    21661 tcatctcaat ctttccgttt aaatcctgcc atacatgatc agcagtagta gggcttggac
    21721 ggccggaagt attggcgctt ggcgctgcta ccggcacacc agccagtgcg atcagacggt
    21781 ttgcaaccgg atcaactggc atacgcactg ctactgtatc cagtccacct gtggttccat
    21841 aaggtacaat atcactcttt gggaagataa tagtcaacgg acctggccag aacgcatcca
    21901 ttaatttctt tcctgcttcc gggatatatt ttaccagtgg tgtaacttcc gctgcacagg
    21961 atacgtgagc gatcagaggg ttgtcggaag gtctgccttt tgcagcgtag atctttcctg
    22021 ccgctttctc atccaaggca tttcccccta aaccatatac agtttctgtt ggaaatgcaa
    22081 caagacctcc gtcacgtaag atctgggcag cttcttttaa ttcttcatca tttatatttt
    22141 tggtatcact tattttaatt cgtttggttt ccatagtgaa tttctccgtt tatgtgtgct
    22201 ttttgcttat agactcaggt ttcctgcgct gacgcaggca aaacccacct tcagagtata
    22261 gcatatttcc aaaagaaaaa acagtcgtga gaacactaac attctcacga ctgcctaaaa
    22321 tcagcctgaa gccttttatt ttaaatcttt tatttcccta cagttccaat acggactaac
    22381 gcacggtgaa gctttgcttc tgccagcttt acttctttgt catccatacc ggactggctc
    22441 agtttatctt ctgccctctt ttttgcttct tcagcacggg ctacgtcgat gtcttcgctc
    22501 cactcccagg tagttgggat cagacggcat tcgccgttca tcatggtcag cattccgccg
    22561 atgcaggctg ctttcctttc tgttccgtcc tctaatacta catgggctgt tcccattcca
    22621 atggccgtgc agtaattcat atggcgggcc agaatggcaa ggtctccgtg gatggtacgg
    22681 caggatacgc tctgtacctg gccgtcaaat acatttccgt ccatggtcgc gatctgtaaa
    22741 tggaaggtcg tcatatacct gttcctccct tctactgctg ctgttttgct ttttcgtata
    22801 catcatcaat ggtgcccgca aacaggaagc agctctccgg gatgtcatcg cattcgccgt
    22861 ctaagatctt cttaaatccg cggatagtct ctttcagtgg cacgtactgg cctttcatac
    22921 ctgtgaactg ctctgctacg gagaagctct gggataaaaa tctctgtacc ttacgtgctc
    22981 tggatacggt cagcttatct tcttcagaaa gttcgtccat acccatgatg gcgatgatat
    23041 cctgcagttc cttataacgc tgcagcactc tctgtacgga acgtgctacc tgataatgct
    23101 cttcgcctac tgtctcaggg gacagaatac ggctggtaga atccagagga tctactgcag
    23161 ggtaaatacc ctggctggca atatcacgag aaagtacggt ggttgcatcc agatgggtga
    23221 atgtggttgc aggagccggg tcagttaagt catccgcagg tacatataca gcctgtacgg
    23281 atgtgatgga tcccttcttg gtagaagtga tacgctcctg taaagcaccc atttctgttg
    23341 ccagtgttgg ctggtaacct actgcggaag gcatacggcc aaggagcgcg gaaacctcag
    23401 aaccagcctg ggtgaaacgg aagatgttat caatgaacag aagcacgtcc tgatttttaa
    23461 catcacggaa gtactctgcc atggtaaggc ctgaaagggc aactctcata cgtgcgcccg
    23521 gcggctcgtt catctgacca tatactaagg cagttttatc gataacgccg ctttcctgca
    23581 tttctccgta aaggtcgttt ccttcacggg tacgctctcc tacaccggtg aatacggaca
    23641 gaccgccgtg tgcagttgct acgttgtgga tcagctccat gataaggact gtcttaccta
    23701 cacctgcacc tccgaacaga ccgatcttac caccctttgc ataagggcag atcaggtcta
    23761 cgaccttgat acctgtttcc aggatctcag ttgcaggtgt ctgctcttcg taagacggag
    23821 cctggcggtg gatcgcccag cgctcttttg cttccggagc aggtttattg tctacaggat
    23881 cgcctaacag gttaaataca cgtcccaggc actcatcgcc tacaggtacg gtaatagggc
    23941 ttcctgtatc tactgcgtct gtaccacgca caagtccatc tgtagaactc atggcgatgc
    24001 agcgcactac gtcatcaccg atctgctgcg caacttctgc gatcagctta tgaccgttga
    24061 tgtcgatctc aatcgcgttc agcagtgcag gcagttcccc ttccttgtac cggatatcca
    24121 gtaccggtcc ggtaacctgc accacctttc caatgtgttt ctctgtcata tatctatctc
    24181 ctctttaagg ctcctctgca ccagctacga tctcagtgat ctcctgggtt atgctggcct
    24241 gacgggcacg gttgtaatac aggctgagct gttcgatcat ctctcctgca ttcttggatg
    24301 cggcatccat tgctgtcctt cgcgctgcca gctcactggc aacagactcg cacatggcac
    24361 catataccat accagccaga tactccggca cgatggcgtc aaacaccgtt tcgctgtccg
    24421 gctcatataa gatcagggaa tggatctgtt tcatatcctt ttcttcccct gaaaggtctt
    24481 ctaacggaag catggatgat acatccggct tctgggaaag catggagaca aagttggtat
    24541 agcccagggc tgcatgaccg aattcaccgg ccttgaatgc cctgcacagt aaggctgcga
    24601 tctcaaagca gtcagataca gagatatctc ctgcttctgc gaagtcttcc gttaccagtt
    24661 ctatgcccct gcgcttgtag tattccactg ctttctttcc gattggaagt acgcagtagt
    24721 ctttcccggc agtttcgctc tccatttcct taaacaggtt ggaattgtat ccacctgcca
    24781 gtcctctgtc acctgcgatc actacatagc accatttttc acttttgctc ttctttgtat
    24841 acacggaaga aaggtctgtg ttgcctgcag cgatctgttt cagtgtgttt tccagagtgt
    24901 ggaaataagg ccggcttgcg tctgcccgtt cctttgcctt acgcagcttg gaggatgcca
    24961 ccagttccat tgctttggtg atctgcatgg tgctctgtat gctcttcata cggagcttca
    25021 cggctttcat atttgcagcc atgtgttccc tccttataat tcatatcatc agcccccgcg
    25081 cccccaaaat gaaggcagca ggggacccga tatcactctt atctggtctt taagaaccgg
    25141 tcggtatagt cagctaaagc agcttttaac ttctcttctg tctcgccttc cagttttccg
    25201 gtggtacgga taccttccat agcagctacg ccctgtgcgt cactgtcaag gaatgtataa
    25261 agtccttctt catatgcctg gatatcttct actgcaacat cagtcaggac accctttaca
    25321 acggcataca ggatagcaac ctgcttctct acaggtacag gtgtacttct tccctgcttt
    25381 aagacttcca cgatacgtgc accctgtgcc agacgtgcct tggtatctgc atccaggtca
    25441 gaaccgaact gtgcgaagct ctgtaactct ctgtactggg agtataccag tttcagggtt
    25501 ccggcaacct tcttcattgc cttgatctgt gcgttaccac caactcgtga tacggagata
    25561 cccgggttta cagctggcat gataccggag tggaacagct ctgtctccag gaagatctgt
    25621 ccgtcggtaa tggaaataac gttggtaggg atgtatgcag atacgtcacc tgcctgtgtc
    25681 tcgatgatcg gaagggctgt aagggaaccg cctccctttt catcagaaag ctttgctgca
    25741 cgctccagta aacgggagtg caggtagaat acgtcaccag gataagcctc acggcccggt
    25801 ggacggcgga tcagaaggga aagtgcacgg tatgcaaccg catgcttact taagtcatca
    25861 tagatgatca gtacgtgttt tcccttgttc ataaagtact cgcccattgc acagcctgag
    25921 taaggtgcaa tgtactgtaa tgggcttgtc tcagatgcag ttgcagcaac aacgatggta
    25981 taatccatag cgccgcctgc ctgcagttca gctaccaggc tggcaacagt agaacgtttc
    26041 tgtccgatag caacataaat acagataacg tctttgccct tctggtttaa aatggtatca
    26101 gtagcgatgg tggtcttacc agtctgacgg tcgccgatga ccagctctcg ctgtcctcta
    26161 ccgattggga tcatggagtc gattgccttg ataccagtct gtaatggttc tttaaccggc
    26221 tgtctgtcgc agatgccggg tgccggtgat tctaccggtc tgtattcggt tgtctcaata
    26281 gggcctgcgc catcgattgg ctggccgatc gcatttacaa cacgtccgat cattgcctcg
    26341 cctacaggta cgcttactac ttttcctgta cgcttaacag tctgtccttc actgatcccc
    26401 tgatcagaac cgaacagaac gacggaaacg ctgttttctt ccaggttctg agccattccg
    26461 aaggtgccat cttcaaactc cagcagctct cctgacatac agttggtcag gccgcttact
    26521 ctggcaatac catcacctac gatcagaacg gttcctgttt cgctctgctc aatagcattc
    26581 tggtaatatt tgatctggct tcggatgact ttggatatct cttccggttt taattgcatt
    26641 ccattcacat cctttatacg atgatttccg taactttttt cttcagactt gacagacgct
    26701 cctgtacggt gccgtcaagc tgctttcctt ctaactccac acggataccg ccaagtacgg
    26761 tgctgtccgt tttctgtgta agaaggacgg tcttgccgct gattttttcc agtctttctt
    26821 ttaatgcctg tgcctgtgct tctgtaaggg ctactgctgt agtgaccact gcttccgcaa
    26881 tgttgtggtc ctcgttgtag cgcgccttaa actgtctgca gctgtcacgg tattcccgca
    26941 gcatgccgct ttcgcataac agctttaaga agtttaacag atacagggaa agctgtcctt
    27001 caaaagcctt gtccaacagc ccgacacgtt ctgttttcgg gatggacggc tcagaaagca
    27061 gggtcaggta atccgggttc tccttaaaga gaactgccac ctgttccatc tgcggaagga
    27121 tctcttccgt cagcttttcc tctgcggcaa ggtcatagag actgtccccg tagatctttg
    27181 cggtcttggt catgatgcct cacctacatt ctcaataaac tcgtcgatca gcttcttatg
    27241 gtcttcttct gagatctcgc gttcaattac cttgccagca atatccatgg caatgccacc
    27301 gatctcattt ttaatgtcat tgactgcttt cttcttctcc tgggcaatat cagcctcagc
    27361 ttttgcctta attccggctg cctgtgtctg agcttcctgg atcatggctt cactgcgtgc
    27421 tgcagcgtcc ttctgggcat tctggagaat ggaatttgca gtttcttttg cttttgccat
    27481 ttcagcctca taatcagcct tcatggcatc cgcttcttcc tttgcctttt tcgcatcctg
    27541 gatctgagaa tcagccaatg ctcttctctt ctcgatgatc gcacatacag gcttgaacaa
    27601 gaacttcttg atcagccagc actgaatgaa caggttgcag atctgggcaa tgaaagtcca
    27661 cggtatgatc gttactaaat cctgtgtttc ccgcatcttt ctaccctcct gtctgtggaa
    27721 accgccttct gtttttcaga agtcttcaca tattatatgc agactttgaa acagtaagtg
    27781 tttctgtaaa aattaggaca ggtagttcat gaaagtacca ggtgctacga agatcagcag
    27841 cagaccggta acgaaaccgt aaataccagt agtctctgcg atcgcacaac caagtaacat
    27901 tgtactggtt acatcgccct tgcactctgg ctggcgtccg attgcttcca gtgcctttgc
    27961 aactgcgtta ccttcaccaa taccaggtcc gatacctgcg atcagagcag caccagcgcc
    28021 gatagcacaa cctgctaaag caataccttt tgctaataac tgaaaatctg tcataataaa
    28081 tttcctccta gaataatgtg aataatatct ttgataagat tttcctactg cgtgtttttt
    28141 taaccttctg ccgcatttgc aatatacatg atcgtcagca tactgaaaat gaatgcctgc
    28201 atgaaaccgg agaaccagtc aaagtagaca gacagaatag ccggaacacc cacatcaagg
    28261 aatgggatgc ttcctaagac ctgtccaagc gcacctggca gccagccaag aagcgctgat
    28321 gttgctaccg ccaaagctgc atagatcagc ccgttgataa caacgccaga aagaatattt
    28381 ccaaagtgac ggcacgccat acttaccggt gttgccagct cagacagaac attgaaaggt
    28441 gtcatgactg ggatcggggt ggtaaatccc tttaagtatc ccaggatgcc gcctgccttg
    28501 atcttattgg aagtgatcat aatgaacact accactgccc aggctgcttc tgtggaaaga
    28561 tcagccgtcg ggcttctaag accggtaagg ctgataagat tggaaactac actggtcgta
    28621 aacaaagctg ccacaaatgg gatcagatta tcaaactttg ttccgccggt attatttctt
    28681 acgaacttct gtgcctgttc taccagcatc tcggccactg cctgccgttt ggatatattc
    28741 tctaccttca ggtccctggt gagccagata cacagtcccg taataagtat cattacgatc
    28801 cagctgacta ccagtgtttc ggatatcagg aaatcgccaa ggatgggtat ccctgtagga
    28861 atcctgaaat aaaccgcagc acctgaaata ttaaaattca tatccgcatt ttcatctcct
    28921 ttcttataaa tttggctttg cttttattat tcccagaatg ttaataagct taatgctcag
    28981 actggggaaa aacagcggta taatgcccgc cgcagcatta acaaatggta ataccattgc
    29041 cagaacgatc cataacaact gggccatgga acgatatctg taacttattt tcatactttt
    29101 gtaggcctgt tcatcactct gttccccgga tgcgatcttc tgcaccgtaa gtcccataat
    29161 aaaaaagttt cctacagaaa cagctgcacc aatgacaccg ccaagaacta ccttgtaatc
    29221 aaatggtact gcatctggaa aaagtttatg gcagactgca aaagcaacga tcataaggat
    29281 cgtacctgca ccactccagc atgcgatatg tcctgtttcc ttctttactg ccggttggat
    29341 cttcatattt ctacctccct gctgcacaca tatgtgtctt cag
//
LOCUS       DBMDUA010000011        27878 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1221_length_27878_cov_4.709652, whole genome shotgun sequence.
ACCESSION   DBMDUA010000011 DBMDUA010000000
VERSION     DBMDUA010000011.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 27878)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 27878)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27878
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1221_length_27878_cov_4.709652"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="ACZPCL_01820"
     CDS             complement(<1..319)
                     /locus_tag="ACZPCL_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HIR1161262.1"
                     /translation="MTYDLNITFDDNLVTGNNTIDTQHKELIDRIRNFVVACQNGDSK
                     VKAIKMLDYLDEYTDFHFKEEEELQEKAGYPERGQHHEKHEEFKKSIQELYEYLQDYE
                     GPTD"
     gene            complement(402..566)
                     /gene="rd"
                     /locus_tag="ACZPCL_01825"
     CDS             complement(402..566)
                     /gene="rd"
                     /locus_tag="ACZPCL_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0072592 - oxygen metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HIR1161263.1"
                     /translation="MKKFECEPCGYIYDPAVGDPDGGIAPGTAFEDIPDDWVCPICGL
                     GKDVFIVVEG"
     gene            913..1863
                     /locus_tag="ACZPCL_01830"
     CDS             913..1863
                     /locus_tag="ACZPCL_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR1161264.1"
                     /translation="MNHKTVVFNEAKMNFDGNLDFSVLSSEVVTYSDTTPDQFLERVA
                     DAEILVTKEMPVSREQILALPDSVKLICEAGTGYNNLDLDAAREKGITVCNIPAYSSE
                     RVAHTAIMMILNLSSTMQTQMAMLAKGNHDNFTKNLQVPHVEVNGKTLGIIGAGNIGR
                     TVMKIAKALDMNILVYTRTPREEEENVHYTDLETVLKNSDYVSLHCPLTPATRHLINA
                     DTLVLMKPTAFLINTSRGALIDEPALIKALQEHKIAGAGLDVQETEPPVEDNPLYTLD
                     NVILTPHMGWKGLETRQRLLSILKGNIDGFINGKPVNVVS"
     gene            1911..2954
                     /locus_tag="ACZPCL_01835"
     CDS             1911..2954
                     /locus_tag="ACZPCL_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIR1161265.1"
                     /translation="MNTHLSFACDYMEGTHPAILQKLVETNLEKTGGYGLDPYCDAAK
                     EKIKAACGCPEGEVFFLVGGTQTNATVIDALLKSYQGVIAAEIGHVSVHEAGAIEADG
                     HKVLTLPHDNGKISADQVRGLLDGFYNDANHDHMVMPGMVYISHPTEYGTLYTLEELT
                     ELSNVCHENHIPLYMDGARLAYALACSENQLTLADIARLCDIFYIGGTKCGALFGEAV
                     VIPKPGLIPHFFTIIKQHGALLAKGRLLGIQFDTLFTDSLYEKVGAPAIQAADQIRKT
                     LKEQNFSLCFGSPTNQVFCIVDDALMTRLEKQVEFGFWEKYDDTHTVIRFATSWATTK
                     EDVDALCNLLCNL"
     gene            complement(3351..4175)
                     /locus_tag="ACZPCL_01840"
     CDS             complement(3351..4175)
                     /locus_tag="ACZPCL_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1161266.1"
                     /translation="MIDQAGILMAQGSGIACERIQGVNDNMSQSHYHEYYEIYYLEAG
                     ERFHMVEDKLYKMEAGEFIIFPPYVMHHSYGAENMPFKRVLLYFSQEEILWPSILSEL
                     REEGGIYKVGIRERQEIHRAMELILKEQKNPGAYHEEYARGVLNMLLLLIAREERPEK
                     APERKSRIGEVIRYIHSHYQEEISLEMLAQMFYVSPYYLCREFKKNTNSTIIRYINVT
                     RIMNAQRKFMETSKNITDISKETGFSNLTHFNRVFKSVTGMSPSQYRKQFRDNVKS"
     gene            4484..6985
                     /locus_tag="ACZPCL_01845"
     CDS             4484..6985
                     /locus_tag="ACZPCL_01845"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HIR1161267.1"
                     /translation="MKVCMNATTVKQKENYFSVATNCTEIRIFFLTDYIIRIRAGFDG
                     DFAEESYSLVTTAWEDRMDPFLKDYRKKIQTADAVLEDGTDQAVIQGKELKVVIEKDP
                     FRICVYDKEGTMIHADIVDLAYREDSNHRRIHTSQIEADDCFFGFGEKGGEFNKAQKY
                     MVLSPGDSMGYDPKETDSLYKHIPFYIKLNQTTKKATGYFYHNTWECDFNMGREKRNY
                     WHRYSSYSTNGGDIDLFLISGPSVRQVVERYTDLTGKSAMLPKYALGYLGSSMYYPEL
                     DADCDDAIAEFIDTTKEEGIPVDGFQLSSGYCAIETEQGIKRCVFTWNKKRFKDPKDF
                     FAQMKKRGICVSPNVKPGILLIHPKLDEMKAKGMFVKASETEEPGIGTWWGGKGMFVD
                     FTSQETRNNWKEMLKENVLDYGTSSIWNDNCEYDSMVDKDCRCSFEGKGGTIGQLKSV
                     MSNIMCHITNEAIHETFDNTRPYVVCRSGHAGIQRYAQTWAGDNLTCWESLKYNIATI
                     LGMGLSGVANQGCDIGGFYGPAPEAELLVRWIQNGIFQPRFSIHSTNIDNTVTEPWMY
                     SNCTEYIRTAIKLRYRLFPYLYSLMERAHETGLPIMEPMCSAFQNDPKCYEEGIDFMF
                     GDSLLVANVVEKGATTRKIYLPEGNKFYDFYTRTPYDGGQTIEIPVDLGSIPLFVKGG
                     SIIPMATNQMKNLMTEQPNGISILCAPDCDGSFVLYEDDGVSMDYEKGCYLKTFISMA
                     AGERTVLTFKQEGSYTTSVETMSLDMIHREKAPYWVTVDGKEIPHFLHRRKFEETDCG
                     WYYSQTLKSVLVKYPNPKKDYQVVVSFEQFDLIGM"
     gene            7008..8030
                     /locus_tag="ACZPCL_01850"
     CDS             7008..8030
                     /locus_tag="ACZPCL_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR1161268.1"
                     /translation="MKKLAAIIAAVGLSLCSLTGCAQSGVKNSAQATAENPMILTLAH
                     GLSETHTVHIAMTEFADKVRERTNGRIQIQIFPNGQLGSENENMEQLMAGVISMTKVS
                     APGLATYNESYHTFGLPYIFDSTEDFYHVMDSDQMHDFFLSSEDDGFVTLTYYTSGAR
                     SFYTKNKAIRKPEDLKGLKIRVQDMKSQTDMMKALGGIPVAMSYGDVYTSLQTGIIDG
                     TENNETALTTGKHGEICKVYSTDQHAMIPDVMVMSAKVWKEISPEDQQIILEAAREST
                     ESHKIAWDTAIDQAIEEAKNTMGVEFVNDVDKEAFREATSGMINDYCTQYPGVKKLLD
                     IIDSVE"
     gene            8043..8546
                     /locus_tag="ACZPCL_01855"
     CDS             8043..8546
                     /locus_tag="ACZPCL_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR1161269.1"
                     /translation="MREFLALIKKWMTRLLAGIATVLLSIMTLLVLYQVFTRYILNSP
                     AAFTEELVRYFLIWTGFIGAAYAFITREHMCLVLVRDSLSPSGKRILMTVIDILILLF
                     AIFVITIGGFKLALSAQKVFSALLGIPRSLVYAMAPISGLFIILAQIINIYEDVTGIA
                     VEGGNES"
     gene            8543..9844
                     /locus_tag="ACZPCL_01860"
     CDS             8543..9844
                     /locus_tag="ACZPCL_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR1161270.1"
                     /translation="MSIGLTTVVLFAVFGILLFLSCPISVSIIIASIVTAISSLSWDQ
                     ITFITMQKMNSGVESFSLLAIPLFILAGNIMNNGGIARRLVNFSKLFVGWIPGSLAQA
                     NIVGNMLFGALSGSSVAAASAMGGCIYPIQKDEGYDPAFATAVNIASAPTGLLIPPTS
                     AFIVYSTVAGGVSISTLFMAGYIPGILMGVGSMVVAFIYAKKHKYPTSGKTPMKEAIK
                     VTLDAIPSLLLIIIVIGGIVGGIFTATEGAGICVLYCLILSICYRSITVKSFMKILVS
                     SACTSGIILFLISASSAMSFVMAYSGIPAAISTGIMSISTNKYVILLLMNIILMVVGM
                     FMDITPAILIFTPIFLPIAQSLGMTDIQFGVMLIFNMCLGNITPPVGSVLFVGCGISK
                     ISIEEVTKTLLPYFAVLFLLLLAVTYIPALSLGVPKLMGLI"
     gene            10082..10975
                     /locus_tag="ACZPCL_01865"
     CDS             10082..10975
                     /locus_tag="ACZPCL_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3881 family protein"
                     /protein_id="HIR1161271.1"
                     /translation="MHKFMRTIGFGGCDSDLEMDKIMRKLAKTASKTAVLERNDHPTL
                     YDLRAELAPGMGIAMIGQMTEKGTFKREYAFPYVTGSDISSTAECSIQRHAERETYAG
                     LLDEYRVGISLIFYMTNSIEYRSRRSKRLPVLAKNICLSGLASQGKILLPVKKTPKQA
                     EDLKQAARKRGSLLEAARRGDEQAIETLTVEDIDLYSMVTKRIIKEDMYSIIETCFMP
                     SGIECDQYSIIGNILSVNLVANDLTNEEIYRLRIECNDMIFTIAINKADLLGEPAPGR
                     RFKGQIWMQGIADFGVNDMLY"
     gene            complement(11017..11961)
                     /locus_tag="ACZPCL_01870"
     CDS             complement(11017..11961)
                     /locus_tag="ACZPCL_01870"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1161272.1"
                     /translation="MKVVFDMDTGVDDSLALIYALNRPAYEIQGIGCVYGNVESWLAA
                     ENTMKILDLVNAPDIPVVIGANEPLTGKWAGRVSIIHGENGLGNVELPKSERKPFDKG
                     IEEFYLETAERLQGEMVLITVGPLTNIARTIKKYPQFVKLVKHMYMMGGTLSMRGNVS
                     PVAEANVACDPVAADYVFQSGMKITAVGLDVTMKVRFKRAYLDWIQTHCRPELKRAAE
                     YLGKAFEWYFMGNQDRNYCMDDSPLHDPLAVMCASVPGLVLTQTRKARVECEGQYCRG
                     MIVTDMREHPFEAETVEFAVEVDEKKAIGELLAAFWEK"
     gene            complement(11961..12662)
                     /gene="deoD"
                     /locus_tag="ACZPCL_01875"
     CDS             complement(11961..12662)
                     /gene="deoD"
                     /locus_tag="ACZPCL_01875"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR1161273.1"
                     /translation="MKTFHIEAEEGQIAKTVLMPGDPMRAKYIAEHYLTDVICYSQVR
                     GMNGYTGYYNGKKVSVQGSGMGIPSMGIYSYELFNTFGVDQIIRVGTCGAVNRDVEVG
                     SLILAMGCCSNSNFASQYHLPGIFAPLADYGLLESAVNQARILEAPFYVGNVYSSDVF
                     YEDLAGEKGQWEKMGVLAQEMETMSLYCTAARAGKKALTMLTVGSSIHSSKALTNEER
                     EKNLDTMIRCALEVA"
     gene            complement(12766..13527)
                     /locus_tag="ACZPCL_01880"
     CDS             complement(12766..13527)
                     /locus_tag="ACZPCL_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1161274.1"
                     /translation="MKENVIKEKNVKIPLYVTVYETISEWLKEGKYKPGDKLPGENIL
                     AEQLNVSRGTLRQAMLLLQEDGFIVNQQGRGNIVLSAQRPAKAGLEKIENPLVDFCLQ
                     PVDKVETTVGFQPATQKHQEVLKLRASSIVAVINITYYSGDTVVGFAMVYMPYEVLDK
                     SSVDLENPDTVYQFYSKLLTSGGLYSDTKLRLGNARERLAGILNIPEGEPFLILEEEL
                     YSEYDTPVLSQKLFFTPDQHELHINRRNDRNIQKH"
     gene            complement(13591..14496)
                     /locus_tag="ACZPCL_01885"
     CDS             complement(13591..14496)
                     /locus_tag="ACZPCL_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161275.1"
                     /translation="MGMINNFLALTLQLSVPIILGALCGTIAERGGVVMLGVEGMMLI
                     GSFAGAVGSYFTGSALAGAVISVVLGAVMGWIYGLFCLKWKAQQSVVGVGVNLFASGI
                     TAVMLKAIWNTEGMSGSVPSISNITVPGLCKVPVLGAFFSEQSPYLYLTLLITLGVWV
                     VFYKTKYGLRYRAIGDQPFAVQTAGVDVNRYRYVAMMTAGAIAGLGGSYLSISQNNLF
                     VIDMVAGRGFMGLAANIFGGWQPLGSLGAGMVFAVAQAARFYLTGQAIPSQFVQMLPY
                     VVTLVVLVVVGKRVKGPEALGKLVD"
     gene            complement(14498..15532)
                     /locus_tag="ACZPCL_01890"
     CDS             complement(14498..15532)
                     /locus_tag="ACZPCL_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161276.1"
                     /translation="MKWISSKQAAELGKSLAAIVLALVIGALFILVAGESPATAYSSL
                     LKGALGTPQAVANTVSKSIPLAFTGLAVALGSRCGMLNIGAEGQLHAGAMAATLTGLT
                     LSFLPAPILLVVSIAAGIAAGMLVGSVPGIFKAKFQTNEVIVAIMLNYVCTLFTSWLV
                     NGPFKVPGSTAQTEMLPENIWFARLLPKTQLTWALFLLLATAVLMYIFLWKTSVGYQL
                     RAVGANASAAKTAGIPVNFYLIMAMTLSGGIAALAGVTEIFGKYHRFIEGFSPSFGFT
                     GIAVAILGKNHPAGVLLTSLLFGVLEMGSLRMSRETAVSTNMVTVIQSLVILFVAAPE
                     LIRWSRKRRG"
     gene            complement(15513..17033)
                     /locus_tag="ACZPCL_01895"
     CDS             complement(15513..17033)
                     /locus_tag="ACZPCL_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1161277.1"
                     /translation="MDAIRMMGIVKCFGPVRANDGIDLIVKQQEIHCLLGENGTGKST
                     LMNILFGLYHPDAGEIYINEKKASITNPNDAYALGIGMVHQHFMLVNQLTVLENIILG
                     KESGGFLLNRKESRKKVEELVERFGFRLPLDEKVENLSVGMKQRVEILKTLYRGADII
                     ILDEPTAVLTPQEVEELFKILRQLKKNGKTIIFITHKLNETMSLSDRITVIRKGKVVF
                     NCDTCDTNEKELATQMVGRQVESIVTKAEDPHGPVVLELKNVRLHKRAQETVSIQVHA
                     GEIFGIAGVDGNGQQELEEMIVGNRKTEEGTISMNGKEVQNLSVKERKAMGLGYIPSD
                     RHKDAMVPDFSITENFLLGYQEDPKYCKKGFIDYKALEEDAKVQVEQFEIKIAGVSQH
                     IGQLSGGNQQKVILGRETSHDPSFMLVAQPVRGLDIGAIERVHKTLLQLKKEGKAILL
                     ISAELSEVMNLSDRIAVLYEGEMSAQFKAGQYTKEEIGLFMAGKKQEDVANEMDQQ"
     gene            complement(17122..18234)
                     /locus_tag="ACZPCL_01900"
     CDS             complement(17122..18234)
                     /locus_tag="ACZPCL_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR1161278.1"
                     /translation="MRKNIFTKATMRRAAAFGLAGCMAAASLTGCGSSKTESTTAANA
                     ETKVEAEGESKAEAADGSDIHVGIIFTEAGLSGNSFNDLALEGVKKAADDYGITYDEV
                     EPKSVSDEEIIQDEMASSGEYDLIICVGAEQVDALTNVATTYPDQKFALLDANSDLDN
                     VASYSCKEQEGAFLAGALAVLAKQEKIDDKLNDGKVIGFIGGVDNPLINHFAAGYQAG
                     AQYIDPDMQVLIDYAGSFNDPTTAKTIANTFVEKGADVIFHASGASGMGMFQAAEEKG
                     FVAIGVNLNQNEIAPDYIMASMLKKLDSCAYHAITSVVEGTYTGENQVLGLSDGGVDV
                     TTEKSNIQVSDSIKEQLDDLKAKVISGEIEVPYELK"
     gene            18700..18776
                     /locus_tag="ACZPCL_01905"
     tRNA            18700..18776
                     /locus_tag="ACZPCL_01905"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18734..18736,aa:Arg,seq:cct)
     gene            complement(19125..20516)
                     /locus_tag="ACZPCL_01910"
     CDS             complement(19125..20516)
                     /locus_tag="ACZPCL_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1161279.1"
                     /translation="MASIVKRKSKYSVVYDYTDENGKRRQRWETFSTNAEAKKRKKQI
                     EYEQDSGTFFIPTAKTLNDLLDEYMSIYGVNTWAMSTYESRRGLARNYITPIIGDMLL
                     SDITPRMMDKYYRDLLSVKTVSVNNRKPTSEYLTPHTVREIHKLLRSAFNQAVRWELI
                     SRNPVLNATLPKEEHKERDIWTAETLSKAMEVCDDPILSLALNLAFSCSLRIGEMLGL
                     TWDCIDIAPQSIENGSAYIFVNKELQRVTRGALDDLSDKGVIKKFPPCIASTHTALVL
                     KEPKTKTSIRRVYLPKTVAYMLVERKKEIDELMDLFGDEYIDNNLVFCSSNGRPMESQ
                     VINRAFNKLIKENGLPHVVFHSLRHSSITYKLKLNGGDMKSVQGDSGHAQVKMVADVY
                     SHIIDEDRCINAQRLEEAFYSSKTPDPVEDTEPKTADTAVTESDESDAAKILELLKNP
                     ETAALLKQLAKAL"
     gene            complement(20494..20931)
                     /locus_tag="ACZPCL_01915"
     CDS             complement(20494..20931)
                     /locus_tag="ACZPCL_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1161280.1"
                     /translation="MQKQRVKTSMSVPEMGKMLGLGKVESYWLVKKNYFKTIQVAGRM
                     RVMLDSFEDWYAGQFHYKKVDGTPPGEKWRHTTMSVPEMADLLGLKSGTAYDLVKRGY
                     FETTLIDRRIRIITSSFEAWYQKQTHYVKISERSNENGIYREA"
     gene            complement(21036..21263)
                     /locus_tag="ACZPCL_01920"
     CDS             complement(21036..21263)
                     /locus_tag="ACZPCL_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1161281.1"
                     /translation="MFEARIAELNRFNEQNPVSYDKRTYTVDEIQDILGISRPTAYNL
                     VKQGVFHSVRVGGHIRISKKSFDDWLDHADE"
     gene            complement(21294..21674)
                     /locus_tag="ACZPCL_01925"
     CDS             complement(21294..21674)
                     /locus_tag="ACZPCL_01925"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR1161282.1"
                     /translation="MKEDWIYKRGDLYYANLNPYFGSEQGGTRPVLVLQNNVGNFFCP
                     TLIVAPLTSKWIKKKELPTHYALESVPELGLKSVVLLEQIKTIDKRRVLSYIGRVSRE
                     EMRAIDDALQVSLDIHIPEEMEAP"
     gene            complement(22162..22710)
                     /locus_tag="ACZPCL_01930"
     CDS             complement(22162..22710)
                     /locus_tag="ACZPCL_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006059623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1161283.1"
                     /translation="MTRVFIWKNNSPQEWEEISFSAFSKARRNGCFTGRFFVETVKMF
                     RDEDDRIIMECSRKDFEKYQQEDRHSRYLQEHEKSRSIFPASHVGDRDGTEEGYQDTD
                     LFVDESVDTAEQAIQNLLLEDLHQALLKLSPAERDFILSYYEMKIPNATCLAQRYGIT
                     RQAADKRLKKIEEKIKKLVAIF"
     gene            complement(23011..23547)
                     /locus_tag="ACZPCL_01935"
     CDS             complement(23011..23547)
                     /locus_tag="ACZPCL_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161284.1"
                     /translation="MRKESTTIQKCNSRLTALGLNSDAAFHRSKLILKIYRDVVWVLS
                     ERAEELQETAWIMGEQDIESGLCYLENFAPDIELQAFEEKVCCLVQNQMLVNIIDRAL
                     LRLKRYPDRGELYYEILTKQFIYRFNSTEKELLEELNIERSVFYDRKREAIYLFSVCL
                     FGYSIPEVLEELPRLNPD"
     gene            complement(23652..24266)
                     /locus_tag="ACZPCL_01940"
     CDS             complement(23652..24266)
                     /locus_tag="ACZPCL_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIR1161285.1"
                     /translation="MSIPQIRTEAIEAVGRKILMEYDPSLLSGQPCPVPIETIIETKF
                     DLILEFHTLRKNPKILGETIFDDGAVVLYDQIQRQYRMIAVRAGTILIDERLCDPSKL
                     GRLRFTCAHELAHWVLHKKLYSGTGDVAAYNGNVSSDESHGIIERQADTLASALLMPL
                     PQIKKCFYRLKIGRTDEQLIAEMANIFEVSKQAMQIRLKSRNLI"
     gene            complement(24278..24646)
                     /locus_tag="ACZPCL_01945"
     CDS             complement(24278..24646)
                     /locus_tag="ACZPCL_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1161286.1"
                     /translation="MGNITFGSFIAEKRKAHKFNLRDTAKHLNIAYGYLCDIEQSRRP
                     APNGDFVERISAFLNLDKSEHELLLDLAAKSRNTVSADLPDYIMEKDIVRAALRVAKE
                     VDATDEEWQTFMKMLKERKR"
     gene            complement(24778..25740)
                     /locus_tag="ACZPCL_01950"
     CDS             complement(24778..25740)
                     /locus_tag="ACZPCL_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1161287.1"
                     /translation="MQNSTNMRILELLRFLYERTDENHPATVSDIIAHLNGKGIQAVR
                     QTVYADTNALIDAGIDIVVVKSTQNQYFMGNRLFEYPELKMLTDAVASSKIISAKKSE
                     ELVQKLCRLTSLHQAKQLQKFAALSSRVKPHNEKVYYIIDNIQTAIGNHQQIRFQYYE
                     YTQEKKKILKHDGYYYVVNPYALEWKNDHYYLIGFSLKHQKIAHFRVDRLTSVENLET
                     YFMPIEGFDVASYTNKMVDMFTSESSKEVTLLCENELMRVIIDHYGEDAAVDRYDDTH
                     FTAKIEVNPSGTFYGWIFKFKGKIKILSPKECITEMQQIAQEFI"
     gene            complement(25769..25909)
                     /locus_tag="ACZPCL_01955"
     CDS             complement(25769..25909)
                     /locus_tag="ACZPCL_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161288.1"
                     /translation="MNGVVLLVGGLLLVAVIKLIIDRNWIGLLLCAVALLLVFGAGHH
                     TK"
     gene            complement(25910..26788)
                     /locus_tag="ACZPCL_01960"
     CDS             complement(25910..26788)
                     /locus_tag="ACZPCL_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4261 domain-containing protein"
                     /protein_id="HIR1161289.1"
                     /translation="MSNKAFQQNLDDKKGPQPGGPYLIQMLFKEPVEMPDKEKMTAIM
                     EKHIGSTECFCYDKKMAGFAALDHVAEFQDGKCPVQLMVMKCDKFKGKGFDAFLMSQM
                     WDCQEDRDRIFRECKYQVVATDMLTAALPALERANLDADFLEALAELYPTCEAFYFQN
                     CGKLFLAEDVRSHQIEGPDRFIRFGVNVRFFNIEGTEDMLIDTVGMSTLFLPDLQYHF
                     HGMDPNWVVNHAYNAASYILEHDNPIEDGETIDGVADGQMCREIQWKCQYEDALIQPP
                     RGVLDINMGNYASGGR"
     gene            complement(26785..27660)
                     /locus_tag="ACZPCL_01965"
     CDS             complement(26785..27660)
                     /locus_tag="ACZPCL_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spondin"
                     /protein_id="HIR1161290.1"
                     /translation="MEQKRPADIIQELLDYLWNGLGLEEKGWKRLKKGDFKKKMKNGL
                     TYQIWFDRSRYNYIDYEIGHGNVEVGFSCIIKQGDDYLYSFRIEPTTGGSFFRMLTED
                     LRLNTGLLDTFLPLVKANYLDFIDRFEADPVEALQPVCAPFTEAEDYSWFIYVREQMV
                     ERYGTAEQMEEYRRQAELRGTPGHKAKNWMGSMLFHLSHANDVDQAWASSRTREELDQ
                     VVEPFVQAKRQTGQWTQEDEAGYQLYRQETDPKKRTFRVWYLIANPRGLPKEFVQKEL
                     EFRWKLFPEKKEEPK"
     gene            complement(27687..>27878)
                     /locus_tag="ACZPCL_01970"
     CDS             complement(27687..>27878)
                     /locus_tag="ACZPCL_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161291.1"
                     /translation="LGWADTEGYPVDGTVDSDGQYSKADIPEHTQYDTQSLAKFAFSM
                     FWRAMRFAEEQQVPILLDY"
BASE COUNT         7355 a         6542 c         5832 g         8149 t
ORIGIN      
        1 tgtctgtagg tccttcataa tcctgaagat attcatacag ctcctgaatg gacttcttaa
       61 actcttcatg cttctcatga tgctgtccgc gttccggata accggctttt tcctgaagct
      121 cttcctcttc tttgaaatgg aagtcagtgt actcatccag ataatctaac attttaatgg
      181 ctttcacctt gctgtcacca ttctgacagg ctactacgaa atttcggata cggtcgatca
      241 gttctttgtg ctgtgtatca atggtattat ttccggttac taaattgtca tcaaaagtaa
      301 tgtttaaatc gtaagtcata gtctggcctc catttctttt atttgctgtg tttagcttat
      361 ctttctcttg tgtgtttcaa tgtctcagtg cttttccctg cttaaccttc aacaactatg
      421 aaaacatctt ttcctaatcc acaaatcgga catacccagt catccgggat atcttcaaaa
      481 gcagttccag gagcgatacc tccgtccgga tctcctactg ccgggtcgta aatatatcca
      541 catggttcgc attcaaattt cttcatagtc aattctcctt ttcgattttt atttatttct
      601 gttatcagat attccaccgg atcttccggt gttttgtttt tattttgttg ctttgttttg
      661 tatgtctgta ttatacatga taattcttct cttgtcaata ttagtaatca ttattatttt
      721 tattttattt aattttttct tcagtctttg cgcaaaacca aatttgctag tataatgaag
      781 cgtagtgaaa aataagcggc tgcgaagcag acatttattt tttacaagcc attaacgaat
      841 gaaccgtctg cgcaagcaga cagcgaagcg cctcaacaga ccgcaaatat agagcgtaaa
      901 ggagaatgat ccatgaacca caaaaccgtt gttttcaacg aagcaaaaat gaactttgac
      961 ggaaatcttg atttttccgt tctttcttca gaagtagtca cctacagtga caccacacct
     1021 gatcagtttt tagagcgcgt ggcagatgca gaaatccttg ttactaaaga aatgcctgta
     1081 agccgtgaac agatcctggc gctgccggac agcgtgaagc tgatctgcga agcgggaacc
     1141 ggttataaca acctggatct ggatgcagcg cgggaaaagg gaattactgt atgcaatatc
     1201 ccggcttaca gttcggagcg tgtagcacac actgccatta tgatgatctt aaatttaagt
     1261 tccaccatgc agacccagat ggctatgctg gcaaagggaa accatgacaa ttttaccaaa
     1321 aatctccagg ttcctcatgt ggaagtaaac ggaaaaactc tgggtatcat cggcgccgga
     1381 aatattggac gcactgtgat gaagattgca aaagcattgg atatgaacat tcttgtgtat
     1441 acaagaactc ctagagaaga ggaggaaaat gtacattata ctgatctgga aacggtactg
     1501 aaaaacagcg actatgtttc ccttcactgc ccgctgacac cagctaccag acatctgatc
     1561 aatgctgata ctctggtgct gatgaagcca actgctttcc tgatcaatac atccagaggc
     1621 gctctgattg atgaaccggc actgatcaaa gctttacagg agcacaagat cgcaggtgcc
     1681 ggactggatg tacaggaaac agaaccgcct gtagaagaca atccgttata cacactggat
     1741 aatgtgatcc ttacacctca catgggctgg aagggcctgg agacccgaca gagactttta
     1801 agcatcttaa agggaaatat tgatggattt atcaacggaa agcctgtaaa tgtggtttca
     1861 taaaaccggt cattttatat ttacattaac gaagggataa taaaaatact atgaacacac
     1921 atttatcttt tgcctgtgac tatatggaag ggactcatcc ggccattctt cagaaacttg
     1981 tagagacaaa tcttgaaaaa acaggtggat atggactgga tccatattgt gacgctgcca
     2041 aagaaaagat caaagcggcc tgtggctgtc cggaaggaga agttttcttt ctggtaggcg
     2101 gcacccagac caatgctact gtaatcgatg cactgcttaa aagctatcag ggtgtgatcg
     2161 cagcagagat cggacatgta agcgtccacg aagccggtgc tattgaggca gacggacaca
     2221 aggtactcac tctgccacat gacaatggaa agatcagcgc ggaccaggtt cgcggacttt
     2281 tagacggctt ctataatgat gccaaccacg atcacatggt catgccggga atggtatata
     2341 tttcccatcc aacggaatac ggcacccttt acaccttaga agagctgact gagctcagca
     2401 atgtatgcca cgaaaaccac attcctcttt atatggatgg ggcccgtctg gcttatgcac
     2461 tagcatgcag cgaaaaccag ctgacactgg ccgatattgc ccgtttatgc gatattttct
     2521 atatcggcgg aacaaaatgc ggcgcactgt ttggcgaagc tgtggtcatt ccgaaaccag
     2581 gtctgatccc gcatttcttt accatcatca aacagcacgg ggcattactt gccaaaggtc
     2641 gtcttctggg gatccagttt gataccttat ttacagactc tctctatgaa aaagtaggcg
     2701 caccggcgat ccaggctgct gaccagatac gaaagacttt aaaagagcag aattttagcc
     2761 tctgcttcgg ttcacctacc aaccaggtat tctgtattgt agatgacgct cttatgaccc
     2821 gtctggaaaa acaggttgaa tttggtttct gggagaaata tgatgatacc catacggtga
     2881 tccgttttgc caccagctgg gcgactacaa aagaagatgt ggatgcatta tgtaatctgt
     2941 tatgcaattt ataaaaaagg ggagattagg ttgagccggc aaggctgccc atttcgtcaa
     3001 ccccgccggc tgtgtaatgt tcgatcggag tcttccatgc acctgccttt gtggctgatt
     3061 ttccgccaac tgcgtccaga ttttatcaac gtacgcaccg cgtacgcctc aaaaatctga
     3121 acacagatga caaaaaatcc tctcacaaaa tcaggcacat aaagattcca atcgaacata
     3181 tttttctcta tagacagata cctctcactc tgctgaacgg gtatctgtct ttctctatgt
     3241 aaaaagctct gactgcttgt ttatgtaaaa gagtatacag aaaagttgtg tcctaagttt
     3301 gaagcagtca taaagtattt ctaaaaaggt ttaagaaaat atgaggaaaa ttatgatttt
     3361 acattatccc tgaactgctt tcggtactga gaagggctca taccagtcac agatttaaat
     3421 accctgttaa aatgagtcag gtttgaaaag cctgtttctt tacttatgtc tgtaatattc
     3481 ttacttgttt ccataaattt acgctgtgca ttcataatac gtgttacatt aatatagcgg
     3541 atgatcgtac tattggtatt ctttttaaat tcgcggcaca gataatatgg gctgacataa
     3601 aacatctgtg ccagcatttc aagactgatc tcttcctgat aatggctgtg gatataacgg
     3661 atcacctcac cgatcctgct ttttctttca ggcgcttttt ccggtctttc ttcccttgcg
     3721 atcaacaata acagcatatt taataccccg cgggcatatt cttcgtgata tgcccctgga
     3781 ttcttctgtt cttttaggat aagctccatt gcccggtgga tctcctggcg ttcccttata
     3841 cccactttat aaatgccgcc ttcctctctt aattcagaaa ggatggaggg ccacaatatt
     3901 tcctcctgtg aaaaatacag cagtactctt ttaaacggca tattctccgc tccataggaa
     3961 tgatgcatta cataaggagg aaaaattata aattctccag cttccatttt ataaagcttg
     4021 tcttctacca tatgaaaacg ttctccggct tccagataat aaatctcata atattcatga
     4081 taatgagact gggacatatt gtcatttact ccctgaatcc gctcacaggc tattccactc
     4141 ccctgggcca tcaggattcc tgcctgatct atcatattcc atcctcctgt ctgtgcaata
     4201 ttgttttcct atatctattt atatggttat tttttaatga agttgtgttt attgtataca
     4261 atttttatgt acttataaca atttttcttt ttgttaaatg tcctttttct cgtacattat
     4321 agcaagatat gtaaaatttc cacctgtaat atgcaatttt tgtctagttc ttatcttgtt
     4381 attttattat catttcaatg caaaacaaca aataaatatc ttagaaaatc atcatatcac
     4441 aaaacaacca taaagttaat tatgtttaag gaggccaaga ttcatgaaag tatgcatgaa
     4501 tgcgacaact gtaaaacaga aggaaaatta cttttctgta gctaccaact gcacagagat
     4561 ccggattttc ttcctcaccg attatatcat ccgtatcaga gccggatttg acggtgattt
     4621 tgcagaagaa tcctacagtc ttgttacaac tgcctgggaa gacagaatgg atccgttttt
     4681 aaaggactac cgtaaaaaaa tccagaccgc tgacgctgtt ctggaagatg gaacagatca
     4741 ggccgtgatc cagggaaaag aactgaaagt agttatagaa aaagatcctt tccgcatctg
     4801 tgtttacgat aaagaaggaa ccatgatcca tgctgatatt gtagatcttg cttaccgtga
     4861 agacagcaac caccgcagga tccataccag ccagattgaa gcagatgatt gtttctttgg
     4921 ttttggagaa aaaggcggtg aatttaacaa agcccagaaa tacatggttt taagcccggg
     4981 tgattccatg ggatatgacc ccaaagaaac cgattctctt tataagcaca taccatttta
     5041 tattaagtta aatcagacta ccaaaaaagc aaccggatat ttctatcaca atacctggga
     5101 atgtgacttt aacatgggac gtgaaaagcg caattactgg caccgttaca gcagctactc
     5161 caccaacggc ggagatattg accttttcct gatctcagga ccttcagtcc gccaggttgt
     5221 agaacgctat accgatctta ccggaaagtc agccatgctt cctaaatacg ctttaggata
     5281 tcttggctcc tccatgtatt atccagaact ggatgctgac tgcgatgatg ctattgcaga
     5341 gttcatcgac actaccaaag aagagggaat tcctgtggat ggtttccagc tttcttccgg
     5401 ttactgtgcc attgaaacag aacagggcat caaacgctgc gtatttacat ggaataaaaa
     5461 acgcttcaaa gatccaaaag acttttttgc ccagatgaaa aaacgtggca tctgtgtatc
     5521 accaaatgta aagccgggaa ttcttctgat ccatccaaaa ctggatgaaa tgaaagcaaa
     5581 aggcatgttt gtaaaagcca gtgaaacaga agaacctggt atcggcacct ggtggggcgg
     5641 aaaaggcatg tttgtagact ttaccagcca ggaaacccgc aataactgga aggaaatgtt
     5701 aaaagagaat gtcctggatt atggtacaag ttccatatgg aatgacaact gcgaatatga
     5761 cagcatggtt gataaagact gccgttgctc ttttgaagga aaaggtggaa ccattggcca
     5821 gttaaagtct gttatgtcca atattatgtg ccacatcacc aatgaagcga tacatgaaac
     5881 ctttgacaat acccgccctt acgttgtatg ccgttccggt catgcaggca tccagcgcta
     5941 tgctcagacc tgggccggtg ataacttaac ctgttgggaa tctttaaaat acaatattgc
     6001 cacgatcctt ggtatgggat tatctggtgt tgcaaaccag ggctgcgata ttggcggctt
     6061 ctacggacct gctcctgaag cagagctgtt agtcagatgg atccagaatg gtattttcca
     6121 gcctcgtttc tctatccatt ccacgaatat tgacaacacg gttacagaac cttggatgta
     6181 cagcaactgc acagaatata tcagaaccgc tatcaaactc cgttaccgcc tgttcccata
     6241 tctgtattcc cttatggaac gtgctcatga aaccggactt cctattatgg agcctatgtg
     6301 cagtgctttc caaaatgacc cgaagtgcta tgaagaaggc attgacttca tgtttggtga
     6361 ctcactgctg gtagcaaatg tagtagaaaa aggcgccaca acacgtaaga tctacctgcc
     6421 ggaaggaaat aaattttatg atttctatac aaggactcct tacgatggtg gacagacgat
     6481 tgaaataccg gtagaccttg gaagtatccc tctgtttgta aaaggcggca gtatcattcc
     6541 aatggcaacg aaccagatga aaaatcttat gaccgaacag ccaaacggaa tttctattct
     6601 ttgcgcgcct gactgtgatg gttcctttgt cctgtatgaa gatgacggtg tttctatgga
     6661 ttatgaaaag ggctgctatt taaaaacttt catttccatg gctgctgggg agcgcaccgt
     6721 tctcaccttt aaacaggaag gttcctatac tacctctgta gaaaccatgt ctctggacat
     6781 gatccacaga gaaaaagctc catattgggt aacggtagat ggaaaggaaa ttccacattt
     6841 tcttcataga agaaagtttg aggagactga ctgcggttgg tattacagcc agactttaaa
     6901 gtctgtactg gtaaaatatc ctaatccgaa aaaggattac caggttgtcg tttcctttga
     6961 gcagttcgat ctgatcggta tgtaagaaag aaaggggata aaataaaatg aaaaaactgg
     7021 cagcaattat agccgctgta ggactgtctc tgtgcagtct tacaggctgt gctcaaagcg
     7081 gagttaaaaa cagtgcacaa gccactgctg aaaatccaat gatacttaca cttgcccacg
     7141 gtcttagtga aactcataca gtccatattg caatgactga gtttgcagat aaggtaaggg
     7201 aacgtacaaa tggaagaatc cagatccaga tcttcccaaa cggacagctg ggatctgaaa
     7261 atgaaaatat ggagcagctg atggcaggcg ttatctccat gacaaaggta tccgcacctg
     7321 gtcttgctac ttataatgaa tcctaccata ccttcggcct tccatatata tttgacagca
     7381 ccgaagattt ttaccacgta atggattctg accagatgca tgatttcttc ctttcatcgg
     7441 aagatgacgg ctttgtaacg ctgacttatt atacttccgg tgcccgctct ttttacacaa
     7501 aaaataaagc gatccgtaaa ccagaagatt taaaaggctt aaagatccgt gtccaggata
     7561 tgaagtccca gacagatatg atgaaagctt taggcggtat tccagtagct atgtcctatg
     7621 gtgatgttta tacttcttta cagaccggta ttattgacgg aacggaaaat aatgaaaccg
     7681 ctcttaccac aggtaagcac ggtgagatct gcaaagtata ttcaacagat cagcatgcca
     7741 tgatcccgga tgttatggtc atgagtgcca aagtatggaa agagatcagt cctgaagacc
     7801 agcagatcat ccttgaagca gccagagaat ctactgaaag tcacaaaatt gcctgggata
     7861 ctgcaattga ccaggcgatt gaagaagcca agaacacaat gggagttgaa tttgtcaatg
     7921 atgtagacaa agaagccttc cgtgaagcta caagcggcat gatcaatgat tactgtacac
     7981 agtacccagg agttaaaaag cttcttgata ttattgattc agtagaataa gggaggttcg
     8041 tcatgagaga atttttagca ctgatcaaaa aatggatgac ccgcctgtta gccggcatcg
     8101 ctactgtcct tctatccatt atgacactgc tggttttata tcaggtattt acacgttata
     8161 ttttaaacag ccctgctgct tttacagaag aactggtccg ttattttctg atctggaccg
     8221 gcttcatcgg agctgcctat gccttcatca ccagggagca catgtgcctt gtactggtac
     8281 gtgacagctt aagtccttct ggaaaacgta ttttaatgac ggttattgat attctgatcc
     8341 tgctttttgc tatttttgtt atcaccattg gtggatttaa actggcttta agtgcgcaga
     8401 aggtattctc tgccctttta ggtattccaa gaagccttgt atacgctatg gctcctatat
     8461 caggattatt tatcattctg gctcagatca ttaatattta tgaagatgta accggaattg
     8521 cagttgaagg agggaatgaa tcatgagtat cggacttaca accgttgtct tgtttgcagt
     8581 tttcggaatt ttgctgtttt taagctgtcc gatttctgta agtattatta ttgcatctat
     8641 tgtcactgcc atatcctctc tttcatggga tcagatcacc tttatcacca tgcagaagat
     8701 gaacagcggt gtagaaagct tctcccttct tgccattcct ctgtttatcc tggctggaaa
     8761 catcatgaat aatggtggta ttgcaagacg tctggtaaac ttttcaaaac tgtttgtagg
     8821 ctggatccca ggttcactgg ctcaagctaa tattgtggga aatatgcttt tcggagcact
     8881 ttcaggctcc tccgttgccg ctgcatctgc tatgggtggc tgtatctatc ccattcagaa
     8941 agatgaagga tatgatcctg cgtttgcaac tgccgttaat atcgcatctg ctcctaccgg
     9001 cctcctgatc ccacctacca gtgcgtttat cgtatattcc actgtagcag gcggtgtatc
     9061 catttcaacc ttatttatgg ctggctacat tcctggtatc cttatgggtg ttggttccat
     9121 ggtagttgcc tttatttacg caaaaaagca taaataccct acttccggaa aaacacctat
     9181 gaaagaggcg atcaaggtaa ccttagatgc tattccaagc ctgcttctta tcatcattgt
     9241 tatcggcggt attgttggcg gtatttttac cgctacagaa ggtgctggca tttgtgttct
     9301 ttactgcctg atcctctcta tctgttaccg tagtatcact gtaaaatctt ttatgaagat
     9361 ccttgtaagc agtgcttgta ccagtggtat tatcctgttc ctgatctccg catcctctgc
     9421 tatgtctttc gtaatggcat actctggaat tccggcagct atttccaccg gtattatgtc
     9481 catctcaacg aataaatatg tgatccttct tctgatgaat attattttga tggtagttgg
     9541 tatgtttatg gacattacac ctgcaatcct tattttcaca ccaatcttcc tgcctatcgc
     9601 ccagagcctt ggcatgacgg atatccagtt tggtgttatg ctgatcttta atatgtgtct
     9661 tggaaacatc acacctcctg ttggttctgt tcttttcgta ggctgcggaa tcagcaagat
     9721 cagtattgaa gaagtaacaa aaaccctgct tccgtacttt gcggtactgt tccttctgct
     9781 tttagcagtt acctatattc ctgcattatc attaggcgta ccgaagttaa tggggctgat
     9841 ctgatccttt caaaatcatt tccggggctg tttaaaacac agcccctttt ttatatttct
     9901 aagattttat atcaaggtgt ttccattgtt aatactgtta ccgttccgtc atgccgtctt
     9961 cttgtttctt taaaaaatct ctggtaatct aacataaagt aggctataat aaagcgaaaa
    10021 acatttagga aacaataaat aaccatagac atagaaacct tagggaaaga gggtgaagga
    10081 aatgcacaaa tttatgcgca ccattggatt tggcggctgc gacagtgatc tggaaatgga
    10141 taagatcatg cgtaagcttg caaagacagc atcaaaaaca gctgtattag aaagaaatga
    10201 ccatcccaca ctttatgatc tgcgggcgga actggcaccg ggaatgggaa ttgccatgat
    10261 cgggcagatg acagaaaaag ggacttttaa gcgggaatat gcgttccctt atgtaactgg
    10321 cagtgatatt tcatctactg ctgaatgctc catccagcgc catgcggagc gggaaacata
    10381 tgccggtctt ttagatgaat accgcgtagg catttccctt atcttttata tgaccaattc
    10441 cattgaatac aggagccgcc gcagtaagcg tcttccagtg ctggcaaaaa atatctgcct
    10501 ctccggactt gcttcccagg gaaagatcct gctgccggta aaaaagactc ccaaacaggc
    10561 ggaagacttg aaacaggctg ccagaaagcg tggaagcctc ttagaagccg cacgccgggg
    10621 cgatgaacaa gccattgaaa cactgaccgt agaggatatc gacctgtatt ccatggtcac
    10681 caagcgcatt ataaaagagg atatgtactc cattatcgag acctgcttca tgccaagcgg
    10741 tatcgaatgt gaccagtatt ccattatcgg aaatatctta agcgtcaatc tggtggcaaa
    10801 tgatctcaca aacgaagaga tctaccgcct gcgtatagaa tgcaatgata tgatctttac
    10861 aattgccatt aacaaagccg atctcttagg cgaaccggca ccaggcagac gttttaaagg
    10921 tcagatctgg atgcagggaa ttgctgattt cggtgtcaat gatatgttgt attaaactta
    10981 aaataccctc gcaaccgcgg gggtattttg cactttctat ttctcccaga aagctgcaag
    11041 cagctctcca atcgctttct tttcatctac ttccactgca aactccacag tctctgcttc
    11101 aaatggatgt tctctcatat ctgtaacgat cattcccctg caatactggc cttcgcactc
    11161 tactctggct ttgcgggtct gtgtcagtac cagtcccggt acagaggcac acatgacagc
    11221 taaaggatca tgaagagggc tgtcatccat acagtagttt ctgtcctggt ttcccatgaa
    11281 ataccattca aaggcctttc caagatactc tgcagcccgc tttaactccg gtctgcaatg
    11341 tgtctggatc cagtccagat aggcgcgttt aaagcgcacc ttcatggtca cgtccagtcc
    11401 aacagctgtg atcttcattc cagactggaa aacatagtca gctgccaccg ggtcacaggc
    11461 tacatttgcc tctgccaccg gtgatacatt tccacgcatg gaaagtgttc cacccatcat
    11521 gtacatatgt tttaccagtt ttacaaactg gggatatttt ttaatggttc ttgcaatgtt
    11581 ggtaagaggt cctactgtga tcaggaccat ttctccctgt aaacgttctg cggtttccag
    11641 atagaattct tcaattcctt tatcgaatgg cttacgctca ctttttggaa gttctacatt
    11701 tccaagtccg ttttctccat ggataataga cacgcgcccg gcccattttc ctgtaagagg
    11761 ttcattggca ccgataacta ccgggatatc cggcgcattt accaaatcca ggatcttcat
    11821 ggtattttct gctgccagcc atgactccac atttccgtaa acacagccaa tgccctgtat
    11881 ctcataagcg ggtctgttta atgcgtagat aagcgcaagg gagtcatcta cgcctgtatc
    11941 catatcaaaa acgactttca ttaagctacc tccaatgcac accggatcat ggtgtccaga
    12001 tttttctctc tttcctcatt tgtaagggct ttgctgctgt ggatgctgct tcctacagtc
    12061 agcatggtga gcgctttctt tcctgctctg gctgcggtac agtataagga catagtctcc
    12121 atttcctgtg ccagaacgcc cattttctcc cactggccct tttcgcctgc cagatcctca
    12181 taaaatacat cggaactgta tacatttccc acatagaaag gcgcttccag gattcttgcc
    12241 tggttgaccg ctgactctaa cagaccgtaa tcagccagag gtgcaaatat tcccggcaga
    12301 tgatactggg aagcaaaatt ggaattgctg cagcagccca tagccaggat caggcttcct
    12361 acttccacgt cacggtttac agccccgcag gtgcccacgc ggatgatctg atccacgcca
    12421 aaggtattaa acagttcgta ggaatagatg cccatagacg ggatacccat tccgcttccc
    12481 tgtactgaaa ctttctttcc attgtaataa ccggtatatc cgttcatccc acgcacctgg
    12541 ctgtagcaga tcacatccgt cagatagtgt tctgcaatgt attttgcgcg cataggatct
    12601 cctggcataa gaacggtctt tgcgatctgg ccctcttctg cctctatatg aaatgtcttc
    12661 attttttact gctcccatct gtttttatgt atatgtccct tatctttggg ctcataccta
    12721 tccgttgggg cagttttatt ttctggtcaa attccccaac gatgattaat gtttttgaat
    12781 gtttcggtca tttctgcggt taatatgcag ttcatgctgg tccggggtaa agaacagttt
    12841 ctgggaaagg accggggtat catattcgga ataaagctct tcttccagaa ttaaaaatgg
    12901 ctctccttcc gggatattta aaatacctgc cagacgctct ctggcatttc ccagacgcag
    12961 ctttgtatcg ctgtaaaggc cgccggaagt cagcagtttg gaataaaact ggtatactgt
    13021 atccggattc tctaaatcta cactgctctt gtctaatacc tcatatggca tatataccat
    13081 ggcaaaacca acgactgtat caccgctgta gtatgtaata ttgatcacag ccacaatgct
    13141 ggaagcccga agcttcaata cctcctggtg cttctgtgtg gccggctgga agcctacggt
    13201 ggtctctacc ttatctactg gctgcaggca gaaatccacc agcggatttt cgatcttttc
    13261 cagtcctgct tttgccggac gctgggcgga aagtacaata tttcctcttc cctgctgatt
    13321 gacgataaac ccgtcttcct gaagaaggag catagcctga cgcagtgttc ccctgctgac
    13381 atttaactgt tctgccagta tattttcgcc tggaagctta tctcccggct tatattttcc
    13441 ctcttttaac cattcgctga tggtctcata aacagttaca tacaggggga tctttacatt
    13501 tttttcttta ataacgttct ctttcactgg cggtttaact cctttatgtc taattcatgt
    13561 tcatatctgc aattataact aagtatagaa ctaatccacc agtttgccaa gtgcttccgg
    13621 tcccttcaca cgctttccaa ctactaccag gaccactaat gttaccacat atggcagcat
    13681 ctgtacgaac tgggacggga tcgcctgacc ggtaagatag aacctggctg cctgtgctac
    13741 cgcgaatacc attcctgcgc ctaaggagcc aagtggctgc cagccgccga aaatatttgc
    13801 cgcaagaccc atgaaaccgc ggcctgcaac catgtcgata acgaacaggt tattctggga
    13861 aatggacaga taggaaccgc ccagtcctgc aatggcgcct gctgtcatca ttgccacata
    13921 acggtaacgg tttacgtcca caccggctgt ctgcactgca aatggctgat cgccgatggc
    13981 acggtaacgc agaccatatt ttgtcttgta aaataccacc cacacgccca aagtaataag
    14041 cagtgtcagg tacagataag gagactgttc agagaaaaat gctcctaaaa caggcacttt
    14101 acacaatccc ggaacagtaa tgttagagat agacggtaca gaaccgctca ttccctctgt
    14161 attccagatt gcctttaaca taacggctgt aatgccgctt gcaaacaggt ttacaccgac
    14221 accaactaca gactgctggg ctttccattt cagacagaac aggccataga tccagcccat
    14281 tactgcacct aaaacaacgg aaatcacagc tcctgccagt gcagaaccag taaaataaga
    14341 accaacggca ccggcaaagg aaccgatgag catcatgcct tcaacgccca gcataactac
    14401 gccgccgcgc tctgcaatgg tgccgcataa agcacctaaa ataatgggaa cagataactg
    14461 caatgtaagg gcaagaaaat tattgatcat tcccatgtta acccctcctc tttctgctcc
    14521 agcggatcag ctcaggagca gctacaaaca agatgaccag actctggatc accgttacca
    14581 tgttagtgga aacagcggtt tcacggctca tacgcaggga acccatttcc aggacaccaa
    14641 aaagtaaaga cgttaaaaga acacctgccg ggtggttctt tccaagaatg gcaaccgcaa
    14701 taccggtgaa accaaaggaa ggagaaaatc cttcaataaa acgatgatat tttccgaaga
    14761 tctctgtaac accggctaag gcagcgatac cgccgcttaa ggtcatagcc atgatcagat
    14821 agaaatttac aggaataccc gctgttttcg ctgcagatgc attggcacct acggctctta
    14881 actggtatcc tacggatgtt ttccagagga aaatgtacat aagtacggct gttgctaaca
    14941 gtaagaacag cgcccaggtc agttgggtct ttggaagcag tcttgcaaac cagatgttct
    15001 ccggaagcat ttctgtctgt gcagtagaac ccggtacctt aaacggtccg tttaccagcc
    15061 atgatgtaaa taaagtacag acataattta acatgatggc tacaatgact tcgtttgtct
    15121 ggaattttgc cttaaagata cccggcacac ttcctactaa cattccggct gcaataccgg
    15181 ctgcgatact tacaaccaaa agaatcggcg ccggaaggaa actcagtgtc agaccggtca
    15241 gtgtagctgc catggcacct gcatgaagct ggccttctgc accgatattt aacataccgc
    15301 agcgagaacc taaggctact gccagtccgg taaacgctaa cgggatactt ttacttactg
    15361 tattggctac tgcctgaggt gttccaagag cacctttcaa cagggaagaa taggcagttg
    15421 ccggtgactc tccagccaca aggataaaca aggctccgat cacaagagcc aaaacgattg
    15481 ctgcaaggga ttttcccagt tctgcagcct gtttactgct gatccatttc atttgccaca
    15541 tcctcctgtt tttttcctgc cataaataaa ccgatctctt ctttggtgta ctgtccggcc
    15601 ttaaactgtg cgctcatttc tccttcatag agaacagcga tacggtcgct taagttcatg
    15661 acttcagaca gctccgcaga gataagaaga atggcttttc cttccttttt cagctgaagc
    15721 agtgttttgt ggacacgctc aatagcaccg atatccagac cacgtaccgg ctgtgcaacc
    15781 agcataaagg acggatcatg gcttgtctca cggcctaaaa tgaccttctg ctgatttccg
    15841 ccggaaagct gtccaatgtg ctggcttacg cctgcgatct tgatttcaaa ctgctctacc
    15901 tggactttgg catcctcttc cagtgccttg taatcgataa agcctttttt gcagtatttg
    15961 ggatcttcct gataacctaa caggaagttt tctgtaatag aaaaatcagg aaccatagcg
    16021 tctttgtgac ggtcagatgg gatatagccc agtcccattg ccttacgctc ttttacagac
    16081 aggttctgga cttcttttcc gttcatggaa atagttcctt cttctgtctt tctgtttcca
    16141 acgatcattt cttccagttc ctgctggccg tttccatcta caccggcaat accgaagatc
    16201 tctcctgcat gtacctggat gctgacagtc tcctgggcgc gcttgtgcag acgcacattt
    16261 ttaagttcca ggactaccgg accatgagga tcttctgcct tggtaacaat agactcaacc
    16321 tgacggccta ccatctgggt tgccagttct ttttcgtttg tatcacaggt atcgcagtta
    16381 aatacaacct ttccttttcg tataacagtg atacggtcag ataaagacat ggtctcgttt
    16441 aacttgtggg taatgaaaat aatggttttt ccatttttct ttaactggcg gaggatctta
    16501 aacagttcct caacctcctg aggtgtaaga acggctgttg gctcgtccag gataatgata
    16561 tctgcgccgc ggtacagtgt ttttaagatc tctacacgct gcttcatacc tacagaaagg
    16621 ttttccactt tttcatccaa tggaagccgg aaaccgaagc gctctaccag ttcttctact
    16681 tttttgcggc tttcttttct gtttaataaa aatccgccgg actcttttcc taaaataata
    16741 ttttctaaaa cagtcagctg gttcaccagc ataaagtgct ggtggaccat gccgatccct
    16801 aaagcataag catcatttgg atttgtgatc gatgccttct tttcattaat atagatctct
    16861 cctgcatccg ggtgataaag gccgaacagg atgttcatca gggtactttt accagtacca
    16921 ttttcaccca gcagacagtg gatctcctgc tgctttacga tcaggtcgat accgtcattt
    16981 gcccgcaccg gcccgaaaca ttttacgatc cccatcattc tgatcgcatc cataaaaatt
    17041 aaacctcccg caattatgcg ttttgctcac tttcattcat ggtaacatca ccgtcaggtg
    17101 acatgtgtat ctcattcctt attacttcag ttcgtaaggt acttcgatct ctccggaaat
    17161 aacttttgcc tttaagtcat ctaactgctc tttgatgctg tcagaaacct ggatattgct
    17221 cttttcagtg gtaacatcaa caccgccgtc gcttaaacca agaacctggt tctcaccggt
    17281 ataggtgcct tcaacaacgc tggtaattgc atggtatgcg caggaatcta acttctttaa
    17341 catacttgcc atgatgtaat ctggagcgat ctcattctgg tttaagttta cgccgatggc
    17401 tacaaagcct ttttcttctg ctgcctggaa catacccatt cctgatgcgc cggaagcatg
    17461 gaagatcacg tctgcgccct tttcaacgaa ggtatttgca atggtctttg cagtggtagg
    17521 atcgttgaag ctgcctgcat agtcgatcag aacctgcata tccgggtcaa tgtactgtgc
    17581 gccggcctga taacctgctg caaagtggtt gatcagtggg ttgtctacgc cgccgatgaa
    17641 gccgatcact ttaccgtcgt ttaatttatc gtcgattttt tcctgctttg ctaaaacagc
    17701 cagagcgcct gctaagaatg cgccttcctg ctctttacag gaataagaag ctacattgtc
    17761 taaatcggag tttgcgtcta agagagcgaa tttctgatct ggataagtag ttgctacgtt
    17821 ggtcagcgca tctacctgtt cagcgcctac gcagatgatc aggtcatatt cgccggaaga
    17881 agccatttcg tcctggatga tctcttcgtc agatacggat tttggctcaa cttcatcgta
    17941 ggtgatgccg taatcatcag cagccttctt aacgccctct aaagccaggt cgttgaagga
    18001 gttgccgctt aaaccagcct cagtaaagat gatgcctacg tgaatgtcgc ttccgtctgc
    18061 tgcttctgcc ttgctttcgc cttctgcctc gactttggtc tctgcgtttg ctgctgtagt
    18121 gctctctgtc ttagaagagc cgcatcctgt taaagatgct gctgccatac atcctgccag
    18181 tccgaaagct gccgcacgtc tcatggttgc tttggtgaaa atgttctttc tcattgtttt
    18241 ttgtcctccc tttggatcat taaaaataat aaattctaca tgtttttcat tctatctgta
    18301 catgtatatc aagttgttca acaagttgtt tcaaatgtag catatttaat aatacttgta
    18361 aatatgtggt tgacaagctt ttatattttc gttaatccta tacaatttat ctgcattgtt
    18421 tttgtgcaac tttaattatc attttgggaa aattacccag aaatacaaag gacttgcatt
    18481 tttcatggaa aaagggtaga atatttaaag attgtgtttc tgtagctcag taggatagag
    18541 cgttcgcctc ctaagcggtt gttcaatcct cactatttac aatttcattt tctatacgcg
    18601 ccaatagctc agctggatag agcgtccccc tcctaaggtt gtgctctacc agctgcctta
    18661 cggggaaaag tgatcgttgc cagtctcaat ttaatataag tctctgtagc tcagcaggat
    18721 agagcgttcg cctcctaagc gaaaggccgt gggttcgaat cccgccaggg acgccagcaa
    18781 acacgccgaa aacctttgat ttatcagggt tttcggcttt tcttatttct gcggcttttg
    18841 tggctttttt gcaaaaaacc ttggtttgct aacatttgct aacgagattt ggccttttgc
    18901 taagaaaggc ggtaagtcag aggttcgatt tttaccaaag gaacttgcaa acatttgtta
    18961 gcagaaatta gaggaagttg caaacaaaat cgcgaatttg ctaacatcgc tcaaaaatgc
    19021 tttcagaacc ttgctaacaa aaaaagtgtt tggtcactgt gcaaagtggt tcaaacgaag
    19081 aaaacctctc acaaagtgag aggcgttctt ctgagggagg gactttataa agcttttgcc
    19141 agctgcttga gcagtgcggc agtttcggga tttttaagca gttccagtat cttcgctgca
    19201 tcactttcat cactttcagt gacggcagta tccgcagttt taggctctgt atcttcaaca
    19261 gggtcaggag tcttagagga ataaaaagct tcctccaatc gctgagcgtt tatgcaccga
    19321 tcctcgtcga tgatgtgaga atagacatcc gcaaccattt taacctgagc atgaccagag
    19381 tcaccctgga ctgatttcat atcgccgcca ttgagtttca gcttgtaggt gatgctggag
    19441 tggcgaagag aatggaacac aacatgaggc agtccgtttt ctttgataag tttattgaaa
    19501 gcacggttaa tcacctgact ttccatcgga cggccattgc tggagcaaaa gaccaggtta
    19561 ttgtcgatgt attcatctcc aaacagatcc atcagctcat caatttcttt ttttctctct
    19621 accagcatat aggcaactgt tttcggcagg tacacgcggc gaatactggt tttggtttta
    19681 ggctctttca ggaccagagc agtatgggta ctggctatgc aaggtggaaa cttcttaata
    19741 acacctttgt cgctcaaatc gtccaatgca cctcgtgtaa cccgttgcag ctctttgttg
    19801 acgaatatat aagctgaacc attttctatg ctctgtggtg caatatcaat gcagtcccag
    19861 gtaaggccca gcatttcgcc aatgcgtaaa gagcaggaga acgccagatt taaggcaagg
    19921 gagaggatag gatcatcaca gacttccata gccttgctca gcgtttcggc agtccatata
    19981 tcccgttctt tatgttcttc tttgggaagt gtcgcattca ggacagggtt acggctgatt
    20041 agttcccaac gcaccgcctg attaaaggca ctgcgaagta gcttgtgaat ttctcttact
    20101 gtatgcgggg tcagatattc gcttgtgggt ttgcggttgt tgacgcttac cgttttcaca
    20161 gagagcagat cacggtaata cttatccatc atccttggag tgatgtcgga tagtaacata
    20221 tctccgataa taggagttat gtagtttctc gccagaccac ggcggctttc atatgttgac
    20281 attgcccagg tatttacacc atagatggac atatattcat cgagcagatc gttcagggtc
    20341 tttgccgtag gaataaagaa ggttccagag tcctgctcat actcaatttg cttttttcgt
    20401 ttttttgctt ctgcatttgt gctgaaggtt tcccaacgct gtctgcgttt tccgttttca
    20461 tctgtgtaat catacaccac agaatatttg cttttacgct tcacgataga tgccattttc
    20521 attgctcctt tctgagattt tcacataatg agtttgcttt tgataccagg cttcaaaact
    20581 gctggtaatg atacgaattc ttcgatcaat caaggtcgtc tcaaaatagc cccttttaac
    20641 aaggtcataa gctgtgccag atttcagccc caaaagatcc gccatttccg gaactgacat
    20701 cgtagtatgc ctccatttct ctccgggcgg tgtaccatcc acttttttat agtggaactg
    20761 gccggcatac caatcttcaa agctatccag cataactctc attcgtccgg ccacttgaat
    20821 tgttttgaaa tagttctttt taaccaacca gtaggattct actttaccaa gcccaagcat
    20881 tttgcccatc tctggtacag acatactggt ctttactctt tgtttttgca tggcttttgc
    20941 ctcctctata aagtttttct tgctatggcc tcattgtaag agaaagcaga aaaaaagtta
    21001 aggatgtcga tttggagatc ggcatccttg acaaatcatt catctgcgtg atccagccag
    21061 tcatcaaagc tctttttgga aatgcggata tgaccgccga ctctgacgct gtggaataca
    21121 ccctgtttta ccagattata tgctgtggga cgactgatac ccaaaatgtc ctgaatctca
    21181 tcgaccgtat aggttctttt gtcatagctg acaggatttt gttcattaaa gcggttcaat
    21241 tccgcaattc ttgcttcaaa cataaaaacg cctcctttat ccggtaagtg gccttacgga
    21301 gcctccattt cctccggaat atgaatatct aaactaactt gcagagcatc atcaatcgct
    21361 ctcatttctt cgcgagatac gcgcccaatg tacgataaaa cacgcctttt atcaatcgtt
    21421 ttaatttgct ccagcagaac aacagatttc agtccaagct ctggaacgct ctccagtgca
    21481 tagtgtgtgg gcagctcctt tttcttaatc catttactgg tgagtggagc tacgatcaaa
    21541 gtggggcaga aaaaattccc cacattgttt tgaagaacca ggacaggacg ggtgccgcct
    21601 tgctctgatc caaaataagg gttcaaattg gcataatata aatccccacg cttataaatc
    21661 caatcttctt tcatgttcat cctcctatgt tttgagattt gattatgtaa gaaggaggct
    21721 ctgcaaatta cagagtctcc ttcaatttag cgcctgtttt atcccttact tgtccacgac
    21781 accccaggga tttgggtaat cattaagccg caggtggctg gcctgcttct caggagtttc
    21841 acctccactt cgtttggtct tacccatggc ctgcgacggt tattatcacg ctcggactat
    21901 ggctgtaaga gagctttcct ccttgttatg ccatgaccgg atgccatccg atgtattcca
    21961 gcctgatata tttctgataa accgaaaact catctataca gcaaaaatgg gttggcctgt
    22021 tatgcgagtg gcagctctca gaaaatatac cgctgatgta gcggctatta cgcccaaaga
    22081 ggatcaaact taatgattcg tatatgaggt tgtcaaggtg caagtgaaag gatttccctc
    22141 tcacttgcta ctgccacttt tttagaaaat cgcaaccaac ttttttattt tttcttcaat
    22201 ttttttcaat cgcttatctg cggcttgccg agtaatacca tatcgttggg ctaaacaagt
    22261 tgcatttggt attttcattt cataatagga caatataaaa tctcgttctg ccggactcag
    22321 tttcaataga gcttggtgta aatcctcaag aagtaaattc tgaatagctt gttccgcagt
    22381 atcaacagat tcatccacaa acaaatctgt atcctgataa ccttcctctg tgccgtcacg
    22441 atctcccaca tgagaagccg ggaatataga gcgacttttc tcatgttcct ggagatagcg
    22501 gctgtggcgg tcctcctgtt ggtatttttc aaaatctttg cgagaacatt ccatgataat
    22561 gcggtcatct tcatcacgga acattttgac tgtttcaaca aaaaagcgcc cagtaaagca
    22621 accattgcgg cgtgccttac tgaacgctga aaaactgatt tcctcccatt cctgtgggct
    22681 gttatttttc caaatgaata ctctcgtcat atcctttcct ccgttcaaat tcttggtagt
    22741 cagcgaattt gaacggtgtc atggatattt tgctatgtac gcctcccagc gcataccggc
    22801 ctcctaatac cgggcaaaga aaaaggccgg aagtgttttc acctccagcc atgttcctgc
    22861 cgccatattg cagcaggaat gaaaaaagcc cggttaatgc cgggccttat gtagaaaagt
    22921 attcaattta gcgcattcct gccactataa caagtatagc aggtcggaag tcggaaggga
    22981 gtagggatta ggtcggggat ttgtcgggat ttagtcggga tttagtcggg gcagctcctc
    23041 caggacttct ggaatagaat atccaaacaa gcaaacagaa aataaataga tggcttctcg
    23101 ttttcggtca taaaagacac tgcgctctat atttagttct tccagcaatt ctttctctgt
    23161 actgttgaac cgatagataa attgcttagt caaaatttca tagtaaagtt ctcctcgatc
    23221 cggataccgt ttcagacgaa ggagtgcacg gtcaataata ttgaccagca tttgattttg
    23281 aacaaggcag caaacttttt cttcaaaggc ttgcagttca atatctggtg caaaattttc
    23341 aagatagcag aggccagact ctatatcttg ttctcccata atccaagcag tttcttgtaa
    23401 ttcttctgcc cgttcactca gaacccagac tacatcacgg taaatcttta gaatcagctt
    23461 acttctatga aacgcagcgt cgctgttaag ccccaaagct gttagcctgc tgttacattt
    23521 ttggattgtt gtgctttctt ttctcataga acatccctcc ttgttcgcaa tattgcgaac
    23581 atattaggta caaaatagca tagcagagga gcgtgagaga gggcagtgcc tctctctatt
    23641 cccccatttg gttatatcag gtttctggat tttagacgaa tctgcattgc ttgcttggaa
    23701 acctcaaaaa tatttgccat ttcagcaatg agctgttcat ctgtccggcc tattttaagc
    23761 cggtaaaaac attttttgat ctgcggtaaa ggcatcagca gagcagaagc cagagtatcc
    23821 gcctgacgct caattatgcc atgactttcg tcggaagaaa catttccgtt ataagcagca
    23881 acatctccag taccggagta caacttctta tgtagtaccc aatgagccag ctcatgggca
    23941 caggtaaaac ggagccgccc taattttgac ggatcacaaa gccgttcatc aattaagatt
    24001 gtaccggctc ttactgcaat cattcgatac tgtctctgta tctggtcata taagacaaca
    24061 gcaccgtcat cgaaaattgt ttcccctaat atcttggggt tttttcttag tgtatgaaac
    24121 tccaggatta gatcaaactt tgtctcgata atggtttcga ttggcacagg acatggttgc
    24181 ccggatagca gagagggatc atattccatc aggatttttc gccctactgc ctcgattgct
    24241 tctgtacgga tttgtggaat tgacaaagct ctccccccta tcgtttacgc tcctttagca
    24301 ttttcataaa tgtctgccat tcttcatcgg tagcatctac ttcttttgcc acacgcaagg
    24361 ctgcccgaac aatatctttt tccatgatat agtctggcaa atcagcagat actgtattgc
    24421 gtgattttgc agccagatcc aaaagcaact catgttctga tttatccaaa ttcagaaagg
    24481 cagagatgcg ttccacaaaa tctccattgg gtgcaggacg gcggctttgc tcaatatcac
    24541 acagataacc gtaagcaata ttcaaatgct tggctgtatc gcgtaaattg aatttgtgcg
    24601 ccttgcgttt ctcggctata aatgatccaa aagttatatt gcccacagca ttcacctctc
    24661 atttgttcga cgaactgcga acatcttgct tatattgtat atcgaacatc tgttcacttc
    24721 aaggataaaa atttcatttg aaaagaggtg ttaaaaatca ataacacctc ttttgcgcta
    24781 tataaattcc tgagcgattt gctgcatttc cgtaatgcac tctttgggag agagaatttt
    24841 tattttccct ttgaatttga atatccagcc ataaaaagtt ccactggggt tcacttcaat
    24901 tttggctgtg aagtgtgtat catcatacct atcaacagca gcatcttctc cataatgatc
    24961 tatgattact ctcatcaatt cgttttcaca tagaagggtt acttccttcg atgactccga
    25021 tgtaaacata tcaaccattt tatttgtata ggaggctaca tcaaatccct ctatcggcat
    25081 aaagtaagtt tccagatttt ctacacttgt tagccgatct acacggaagt gagcaatttt
    25141 ctgatgcttc agagaaaatc caatcaaata gtaatggtca tttttccatt caagcgcata
    25201 aggatttacg acataataat atccatcatg ctttaagatt ttcttttttt cttgagtata
    25261 ttcatagtat tggaaccgaa tttgctgatg gtttccaatc gctgtttgga tattgtcaat
    25321 gatgtagtaa accttttcat tatgcggttt gacacggctg gataaagcag caaatttttg
    25381 aagctgcttt gcctgatgta gactggtcag acggcataat ttctgcacca gctcctcaga
    25441 cttcttggca gaaatgatct tcgaggatgc tactgcgtct gtcagcattt tcagttctgg
    25501 atactcaaac aggcggtttc ccataaaata ttggttttgg gtactcttaa ccaccacaat
    25561 atcaatcccc gcatcgatca gcgcattggt atctgcgtaa acagtctgcc gcactgcctg
    25621 aattcctttt ccattcagat gggcaatgat gtcagatact gtggcgggat ggttttcatc
    25681 ggtccgctca tagagaaacc ggagtaattc cagtatccgc atatttgttg aattctgcat
    25741 acttcacctc ctcgattctt aaaatggcct attttgtatg gtgtccagcc ccaaagacca
    25801 gaagcagggc gacagcacag agaagcaggc cgatccaatt tcgatctata atcagcttga
    25861 tcaccgccac aagcagaagt ccgcctacca ggagcacaac gccattcatt cagcgtcctc
    25921 ccgaagcata gtttcccatg ttgatgtcca gcacccctct gggcggctgg atcaaggcat
    25981 cttcatattg gcacttccac tggatctccc ggcacatctg gccatccgcc acgccgtcta
    26041 tggtttcccc atcctcaatg gggttatcat gctccagaat ataggacgct gcattatagg
    26101 cgtggtttac cacccagttg gggtccatgc cgtggaagtg gtactggaga tccggcaaaa
    26161 acagggtgct catgcccacc gtgtcgatga gcatatcctc cgtgccctcg atgttgaaga
    26221 aacggacatt gacgccaaag cggatgaacc gatccggccc ctcaatctga tgggagcgca
    26281 catcctctgc cagaaacagc ttgccgcagt tctggaaata gaacgcctca caggtggggt
    26341 acagctctgc cagagcctcc agaaagtcgg catccaggtt ggcacgctcc agcgctggaa
    26401 gcgccgcagt cagcatatcg gtggccacca cctgatattt gcactcccgg aagatccggt
    26461 cacggtcctc ctggcagtcc cacatctggc tcatcagaaa ggcatcaaag cctttgccct
    26521 tgaacttgtc gcatttcatc accatcagct gtaccgggca cttgccgtcc tgaaattccg
    26581 cgacatgatc cagggcggca aagccggcca ttttcttatc ataacagaag cactctgttg
    26641 atccgatgtg cttctccatt atggcagtca ttttttcttt atccggcatt tctaccggct
    26701 ctttgaacag catctgaatg agatagggac ctccgggctg ggggcctttt ttgtcgtcca
    26761 gattttgctg gaaagcttta ttgctcattt gggttcctcc ttcttttctg ggaacagttt
    26821 ccaccgaaac tccagctctt tctggacaaa ctctttcggc aggccgcggg ggttggcgat
    26881 gagataccat acccgaaagg tgcgcttctt cggatctgtt tcctgccggt agagctgata
    26941 gcccgcctca tcctcctgtg tccactgtcc tgtctgccgc ttggcctgca caaagggttc
    27001 caccacctgg tccagttcct ctctggtgcg gcttgaggcc caggcttgat ccacatcatt
    27061 ggcgtgggag aggtggaaca acatcgaacc catccagttc tttgccttgt gtccaggcgt
    27121 tccgcgcaat tccgcctgac ggcggtactc ctccatctgc tccgccgttc cgtaccgttc
    27181 caccatctgc tcccgcacat agatgaacca gctgtagtcc tccgcctcgg tgaagggggc
    27241 gcagaccggc tgtaatgcct ccacggggtc tgcctcgaag cggtcgatga agtcgaggta
    27301 gttggctttg accaggggca gaaaggtgtc cagcagcccg gtgttcagcc gcaggtcctc
    27361 tgtcagcatc cgaaagaatg aaccgcctgt tgtgggttca attctgaaag agtagaggta
    27421 gtcatctccc tgctttatga tacagctgaa gcccacctcc acattcccat ggccgatttc
    27481 gtagtctatg taattgtagc ggctccggtc aaaccagatc tgataggtca gcccgttttt
    27541 cattttcttt ttgaagtcgc cctttttcag acgcttccag cccttttcct ccaggcccag
    27601 gccgttccac aggtagtcca gaagttcctg aattatatct gccggccgtt tttgttccac
    27661 ggtctccacc ccctgacgaa ttctctttaa tagtcaagca ggataggcac ctgctgctcc
    27721 tcggcaaacc gcatggcccg ccagaacatg gagaaggcaa acttggcaag ggattgagtg
    27781 tcatactggg tgtgttccgg gatgtctgcc ttgctgtact gtccatctga gtctacggtt
    27841 ccgtctaccg ggtatccctc cgtgtctgcc cagccaag
//
LOCUS       DBMDUA010000012        27121 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1253_length_27121_cov_4.439193, whole genome shotgun sequence.
ACCESSION   DBMDUA010000012 DBMDUA010000000
VERSION     DBMDUA010000012.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 27121)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 27121)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27121
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1253_length_27121_cov_4.439193"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="ACZPCL_01975"
     CDS             <1..251
                     /locus_tag="ACZPCL_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161292.1"
                     /translation="PLPATQAEPSLIAQEKQMPLLAALVFLLDSLYHELKVWTMYCKH
                     SVKFFKKNIKKIVDKKRMIRYNFRCVQENAPLAQLVEQ"
     gene            222..294
                     /locus_tag="ACZPCL_01980"
     tRNA            222..294
                     /locus_tag="ACZPCL_01980"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:255..257,aa:Lys,seq:ctt)
     gene            complement(734..1396)
                     /gene="trmB"
                     /locus_tag="ACZPCL_01985"
     CDS             complement(734..1396)
                     /gene="trmB"
                     /locus_tag="ACZPCL_01985"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR1161293.1"
                     /translation="MRLRHIRGAEETIATSPYVIQEPELHKGSWNQVFGNDNPIQIEV
                     GMGKGRFIMELAQQNPDINYIGIERYSSVLLRGLQKRAQLELNNIYFMCIDAKNMADY
                     FAPGEVDKIYLNFSDPWPKDRHAKRRLTSPDFMAVYDKILAADGMVEFKTDNKGLFDY
                     SLEAIPEAGWTITAHTFDLHHDPVMCAGNVMTEYEEKFSSMGNPICKLIAMREPKENE
                     EN"
     gene            1736..2959
                     /locus_tag="ACZPCL_01990"
     CDS             1736..2959
                     /locus_tag="ACZPCL_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR1161294.1"
                     /translation="MSNFERTTSWQQIQQGVKEAERLIVSKDYNLVMVKARQTLECMV
                     RCLAEKACLVDGDLADTIDQLYEGHWIDKTTKDDYHTIRILGNKAVHENDNTAYNANQ
                     AYQLLTKQVYAFSHDFQVAKTFTVPVTDTRPVRNARTGATTGSSGAGSAFYGQTGGAR
                     PRTGTGAGQTAASRAQSSVRSQTSPRSQTSARTSNGTGNGPRSGAELQGLSSRSGANG
                     SRRTAGSNSSTGSRNRNSQSTNRSYSGSSRSGRTRRRKHRPSPVAMVLKFLIPILVIV
                     VLVVAIHTLVPGKKKADPTQPETMSTEMTSAETEAPTPEETQPPADVYVVTGNKVNVR
                     TEPSTNGRILVQLEGGTEVTYVKRYNNDWTVINYDGQEAYVSSQYIAKQEAETTAETQ
                     AEAETAAEGETAANQ"
     gene            3197..3508
                     /locus_tag="ACZPCL_01995"
     CDS             3197..3508
                     /locus_tag="ACZPCL_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR1161295.1"
                     /translation="MDTVINRLSDIEKAAVSVMDGASERKKQMAREMEEKTAAFDAQL
                     EKENQDRISQIRSKMEEELQQELRQQSEDAKATMKRLEATYENRHEEYAQALFKNMIK
                     E"
     gene            3513..4532
                     /locus_tag="ACZPCL_02000"
     CDS             3513..4532
                     /locus_tag="ACZPCL_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HIR1161296.1"
                     /translation="MGSLISYSGISTKVKAMERFRIRPEQFEAMADLGSVPEAVQFLR
                     SNPSYEKAFSKASEDELHRGKIEQLLNLSKYHDFAKLYRFANVGQRRFLDLYFMHYEI
                     DILKTCLRNAAGNRESAQDLTGFKEFFDKHSDMDLVQLSQCRSIEDVIRGLKGSAYYE
                     PLEKLLEQGKATLPACETAMDMLYFKTIWKIKDKYLSKAEAKMLAQCFGTKMDMLNFQ
                     WICRAKKYYNLSEGDIYALIIPIQYHVTKKEIQTMAQAENMEQVYGVIKNSWYKCQNI
                     EELMDQGLGNAAKELTDKIYELTSRKEPYSAAVLNSYLYFKEREIEQIITTIEKIRYG
                     MTART"
     gene            4548..6476
                     /locus_tag="ACZPCL_02005"
     CDS             4548..6476
                     /locus_tag="ACZPCL_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HIR1161297.1"
                     /translation="MIEKMKFLSITGPKADIDRVVDTYLSRYEIHLENALSELKTVKD
                     LRPYIETNPYKEKLNIARELAEKIGGQLPEVSETELPTQEEAAKMVDDLNSRLKELND
                     KKEELQAQIQTLKRSKDQVAPFTELNYSVKEILGFQFIKFRFGRISREYFDKFNSYVY
                     ETIDTVMFKCLEDAEYVWIVYFVPEKLADKIDAIYASMHFERCFLPDEYEGTPMEAEH
                     VLDDRIKALEAEKQEVEGKILQTLDENKKELAAARTRLERFSVNFDVRKMAACTKHGD
                     HTFYILCGWMSETDAKKFQKEIESDADTFCIMEDNHNNIMSTPPTKMKNPGLFKPFEM
                     YVEMYGLPSYNEIDPTILIGITYSILFGFMFGDAGQGLCLLIGGFLLYKFKKMRLAGI
                     ISCCGFFSTIFGVLFGSVFGFEDIIDAVWLRPQEAMTDLPFIGKLNTVFVVAIAIGMG
                     IILLCMILNIINSVKEHNTEKTWFDTNGAAGLVFYFALATVIVLYMSGNALPATIVLT
                     VLFIIPLLLMFFKEPLSAMVEKKAQKMEGGVGMFITQGVFELFEVLLSYFSNTLSFVR
                     VGAFAVSHAAMMQVVLMLAGAEAGSPNWAVVIGGNLFVCGMEGLIVGIQVLRLEYYEL
                     FSRFYRGSGRAFEPYGKH"
     gene            6507..6941
                     /locus_tag="ACZPCL_02010"
     CDS             6507..6941
                     /locus_tag="ACZPCL_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HIR1161298.1"
                     /translation="MSLLVKMTLSAALVLSIGVPFGAFAAGEKTKGRYKTALGVNVFL
                     FFGTMIAAAAFMFSGNAFAAEETAAAASNSAAGMGYLSAALATGLSCLGGGIAVSAAA
                     SAALGAISEDSSILGKSLIFVGLAEGVCLYGLIISFMILGKL"
     gene            6943..7251
                     /locus_tag="ACZPCL_02015"
     CDS             6943..7251
                     /locus_tag="ACZPCL_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HIR1161299.1"
                     /translation="MKMYLISDNIDTWTGMRLAGVDGAVVHERDELKRELDKVLADKS
                     IGIILLTEKFGREFPEIIDNVKLERKLPLIVEIPDRHGTGRKPDFITSYVNEAIGLKL
                     "
     gene            7268..7852
                     /locus_tag="ACZPCL_02020"
     CDS             7268..7852
                     /locus_tag="ACZPCL_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HIR1161300.1"
                     /translation="MTTEEKLKHFQEICMTDAREKSAKILDDYAKTLDKAYEEHTEDA
                     RKRAKMQEEAETEKLGRERNKKLSIGQLDLRREVSRRQEELKDKLFVEVRDKLANFME
                     TREYLDLLEKQIGAAKKVAGDEAMIVYMDPSDEDKARRLAMHHNVTVKISEYSFDGGI
                     RAVIPSKHILIDSSFKTKLEEARHEFKFDLGGKQ"
     gene            7853..9619
                     /locus_tag="ACZPCL_02025"
     CDS             7853..9619
                     /locus_tag="ACZPCL_02025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIR1161301.1"
                     /translation="MAKTGVIYGINGPVVSLAGDPGFQMNEMVYVGKENLVGEVIGLT
                     SEKTTVQVYEETSGLKPGEVVTGTGAPVSVTLAPGILDNIFDGIERPLSKIAERSGYY
                     ISRGISVDSLDTSKKWKTHMTVKKGDRVLPGTIIAEVPETRAIIHKIMVPPDTEGYVL
                     DVVEDGEYTINEPLLTLQLLDGTEKKLTMTQKWPIRVPRPSAKRYPATRPLITGQRIL
                     DTMFPLAKGGTAAIPGGFGTGKTMTQHQIAKWSDADVIIYIGCGERGNEMTQVLEEFS
                     ELIDPKSGNPLMDRTTLIANTSNMPVAAREASLYAGVTLAEYYRDMGYHVAIMADSTS
                     RWAEALRELSGRLEEMPAEEGFPAYLASRLSSFYERAGMMRNLNGTEGSVTIIGAVSP
                     QGGDFSEPVTQNTKRFVRCFWGLDKNLSYARHFPAIHWLTSYSEYVNDLSSWYIDNVD
                     KNFVSDRNRLMALLTQESSLMEIVKLIGADVLPDDQKLVLEIARVIRVGFLQQNAFHK
                     DDTSVPLTKQFKMMETILYLYKKSKALIAMGMPMSVLKEDKIFDKIISIKYDVPNDRL
                     DMFDDYKKQIDAFYEHVLERNA"
     gene            9632..11005
                     /locus_tag="ACZPCL_02030"
     CDS             9632..11005
                     /locus_tag="ACZPCL_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HIR1161302.1"
                     /translation="MAVEYLGLSEINGPLVVLEGVQGAAYDEIVEMTVGGTTKKIGRI
                     IEIYGDKAIIQVFEGTDGMALRNTHTRLTGHPMEIAVSEEMLGRTFNGIGQPIDGLGD
                     IISDIRMDINGQPLNPVSREYPRNYIRTGISAIDGLTTLIRGQKLPIFSGNGLPHDQL
                     AAQIVQQASLGDNSDEKFAIVFGAMGVKHDVADFFRRTFEESGVSEHVAMFINLANDP
                     VVERLITPKIALTLAEYLAYEKGMHILVILTDMTSYAEALREVSSSKGEIPSRKGFPG
                     YLYSDLASLYERAGIVAGRNGSVTQIPILTMPNDDITHPIPDLTGYITEGQIVLDRQL
                     NSQSVYPPISVLPSLSRLMKDGIGEGYTRKDHQDVANQLFSCYAKVGDARALASVIGE
                     DELSAIDKKYLEFGKAFEEHFIGQAHHENRSIIETLEIGWKLLRMLPREELDRIDTKV
                     LDQYYEE"
     gene            11017..11634
                     /locus_tag="ACZPCL_02035"
     CDS             11017..11634
                     /locus_tag="ACZPCL_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIR1161303.1"
                     /translation="MDPNTFPTKGNLIAAKNSLKLATMGYGLMDKKRNILIRELMGLI
                     DEAKGIQSEIDTVFKAAYEALQSANIELGINYVQEIGMSVPVDDTVKIKARSIMGTEI
                     PLVQHQEKPMELAYGFNNTSEALDIARERFERVKELTIKLSMVENSAYRLANSIKKTQ
                     KRANALKNITIPHYEELSKSISNALEEKEREEFTRLKVIKRMKTG"
     gene            11701..12081
                     /locus_tag="ACZPCL_02040"
     CDS             11701..12081
                     /locus_tag="ACZPCL_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HIR1161304.1"
                     /translation="MYTFKDLVEITAKLRAEDGCPWDREQTFESLRKYMREEAEEAVQ
                     AVDKNDMENLCEELGDVLFQVMLNSQIAKEKGLFSIDDVIGGICEKMVRRHPWVFGGE
                     KINSTEEGEALWARIKMQEKAKKP"
     gene            12183..12458
                     /locus_tag="ACZPCL_02045"
     CDS             12183..12458
                     /locus_tag="ACZPCL_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR1161305.1"
                     /translation="MNKTELIAAVAEKAEISKKDAEKAVKAFTDAVAEELAKGGKVQL
                     VGFGNFEVSERPAREGRNPRTGETMTIAASKTPKFKPGKALKDEINK"
     gene            12541..12780
                     /locus_tag="ACZPCL_02050"
     CDS             12541..12780
                     /locus_tag="ACZPCL_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR1161306.1"
                     /translation="MRLDKYLKVSRIIKRRTVANEACDSGRVMLNDKVARASAEVKVG
                     DVIEIAFGTKAVKVKVTSVQDAIRKEDAKEMFEYL"
     gene            12972..13628
                     /locus_tag="ACZPCL_02055"
     CDS             12972..13628
                     /locus_tag="ACZPCL_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1161307.1"
                     /translation="MARNTERDEREATRRKEQLMKAGFKLFSENGIENVSLQKVADAA
                     DVGVATMYKYYQTKVKLVVAISGKIWGDIWKSVLAQNGPDFFENFTAYEYIEFYLDMI
                     IRLYREKPEMLRFSNNYKNFIYHEGIEEEPLGEHLDVLEPLRAMYHKLYEQAKVDHSI
                     RTDLSEQELFTTVAIAMLAVAERYAQGIVWANDHKTDHTQELKYLKEMLLMWCKGKTE
                     "
     gene            13728..14117
                     /locus_tag="ACZPCL_02060"
     CDS             13728..14117
                     /locus_tag="ACZPCL_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161308.1"
                     /translation="MNTLFTSRLNGIFILNTLLELHKPVTVSELAGIINHRYDHVTAP
                     RKMVSADTVKRFLNDFYQTTSGTDYCSIRLFCFKKNRYGSLVPDHDLENVTLATYVAA
                     CLELNPSMNVRDGRGLKSNSVVHFHTA"
     gene            complement(14079..15968)
                     /locus_tag="ACZPCL_02065"
     CDS             complement(14079..15968)
                     /locus_tag="ACZPCL_02065"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012234.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1161309.1"
                     /translation="MSEKKNLIVPKLQKQNLGKVNPQKAEYLYISKEFCKEGLSQIIK
                     DGGVQFKEIQKFVKDHQEPQYILVHASKLEEGYLAISLLAGIYNEIHSRREWEEGAEP
                     DIQWSDSWQCLPIIPFKEADDYYNWQENDFSSFGNSFLASQKTNDTFVPYWAENEDKP
                     VCISMENISFFRCDPIIDTLNNFRSNEQVYVLILHEENDFDSFNSFGGFGGFDTFESN
                     EDKNLNQIILSLTAEELEIPKTSSVSLYYENIWKQMVTNSGCKLERKFPVHDFLLDIE
                     KMNKEFSYDFLDKILKYALRNKEHGILRKKDFQFMERFAGKRGPAEQKTETRSAIRCL
                     REDLIGMDQVKEQVMETVQVMKFNRLRKEMGMKKSSYHNVHMMLGAPGTAKTTVAKLM
                     GKIMEEEKLLPGSQFTCVNGAELKGMYVGHSAPKTKALFEENDIIVIDEAYSLTGDRS
                     EPDSFSREAIAQLVIELEEHAEDKLVIFAGYGGTDVDEKNNKMKEFIDANPGIKSRIN
                     STFYFPSYTAPEMAEIFKKHAEMSGYELPENWKEPITAYFSTRVNDENFGNGREARAL
                     FEKVSVQMAKRIMGDANGGLQNSINEKAIKQCRISDIEGAIRRAREENKQISGRVKVH
                     NRIGF"
     gene            16173..17336
                     /locus_tag="ACZPCL_02070"
     CDS             16173..17336
                     /locus_tag="ACZPCL_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024676.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1161310.1"
                     /translation="MASRYNQLTMLYIFNILKLDASPKKPYSAPMVYRRLKELGIDLD
                     RKTVFNHMKILTDQMLSADQDEESFFKQYLCSRLFRCRKEEGKEKSRENGRKPGNESS
                     YRICLPEEEIPENSVLYYYIEDEMSPEELELLQSTVAINQYLSAEKTREILADLEHKK
                     PFDCREQSLYTAFADPRLKNQATDIFASLSVIRDSIRKKKKLRILYGRYDRNLELAPT
                     SEDARTIEPYAVMSSNGYYYLIAGNPKYPDGLTNFRIDRILQIQMLDEPRKPLPSALL
                     PYFRDAAHEVFQASEYRNDHPVMYSDETVRIHLSCDPVILNNLVDDFGWSIQTRDIKD
                     PDHPDWVHVTAKASLMGAAVFCTHHCRHCKVISPQKLREKVVENLKAGLALYE"
     gene            17440..17727
                     /gene="yabP"
                     /locus_tag="ACZPCL_02075"
     CDS             17440..17727
                     /gene="yabP"
                     /locus_tag="ACZPCL_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HIR1161311.1"
                     /translation="MEEKISSGRLHRLVLSDRHTGSVTGVNDVVSFDENEIVLDTDMG
                     LLTVRGKELHVKRLTLEKGELDLEGQVDSLNYSSNAALKRSGDSFLNRLFK"
     gene            17743..18075
                     /gene="yabQ"
                     /locus_tag="ACZPCL_02080"
     CDS             17743..18075
                     /gene="yabQ"
                     /locus_tag="ACZPCL_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HIR1161312.1"
                     /translation="MSMQIRFEAWLLLLSLLVGCWLMLVYDLLRVLRLMIKHSSFVMG
                     IEDFFYWIYAGTATFMLLYELNDGGVRMYAVAGVFAGMILYDKIVSRFLFAALKKAGK
                     WFKIKRNR"
     gene            18095..18412
                     /locus_tag="ACZPCL_02085"
     CDS             18095..18412
                     /locus_tag="ACZPCL_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator"
                     /protein_id="HIR1161313.1"
                     /translation="MSSYGRSKRVKREKWANRMAVLGVTMVVLCLAVAVNAKGSTLKK
                     SDLDYQQKEKQLEEQVREEEERTKKLEEYKVYVKTKQFAEEVAKEKLGLVNPDEILLK
                     PNQ"
     gene            18513..19940
                     /locus_tag="ACZPCL_02090"
     CDS             18513..19940
                     /locus_tag="ACZPCL_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR1161314.1"
                     /translation="MLRRLGGIFVLSGKNENGINYYTARKLKDMAQSLEQLAKAFDEG
                     AGETMNLTKDDGLAAMQTCAALVCGNCGGCSLYADSEKEDSYYLYYLLRIFEQNGRIG
                     TEDMPELFKKDCRRKRDYLEQLNRSFTRASMNLSWKNRFLESRDVVISQFRELAVILE
                     EFSRQIDEAKNITEEYKGAVKKAFRRYHILVDSMLLLEYESGRREIYLTAKTTNGRCV
                     TSKDAAELMESVFPDTTWLPAKDSRSIITRRYETVRFEEKGDYYLTWGAAGVPKQGER
                     YSGDNYSFCERGSCQVMFSLCDGMGSGAVAGQESRRIVELLEALLEAGFAPRSAFKLV
                     NTVLLLAGGEQHPAALDAGCIDLYTGALEVFKLGAAPTFVMGEEGIQVLKAGQVPAGA
                     LGQAEPVLLSQKLWDGDRLVMVTDGVLDALPGEDKEQVMGQFLESLEEMPPQDMAERI
                     LEFALSFVPGARDDMTVLTAQVWKR"
     gene            20093..21469
                     /gene="tilS"
                     /locus_tag="ACZPCL_02095"
     CDS             20093..21469
                     /gene="tilS"
                     /locus_tag="ACZPCL_02095"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR1161315.1"
                     /translation="MEKLEYTVRQTICRYHMLQPGDQVVIALSGGADSVCLLSLLADM
                     REEMGLKLRSLHVHHGLRGEEADRDGDFSIKISEALGVPCHMIKAEVKKYASQTGMSE
                     EEAGRILRYKALEEEAGAWEGENGQTVKIAVAHHSRDQAETILLNLFRGSGLGGLKGI
                     PYVRDRIIRPMLDVSKEDIIPYLEKKRLKWVNDSSNDTDHYARNRVRSHILPAIKEEI
                     NPGAEAGIRRAGYLAGMADVYLKKQADQWVQKYVKEEEHTWMIPEGPFKEADPVIRLY
                     SVMELLKTAAGCAKDLGLVHAEQVEKLVDLQTGRKVSLPYGLEARKVYGGISLQAVKK
                     EPLEERIKNPLPELSFEVFSYEKAMEFPKKTYTKWFDCDKIKGTPVVRTRQTGDYITL
                     ENGSRKPLRRFMIDEKIPADKRDEIALLADGDHIMWIIGRRISSYYKIGPDTRRVLQA
                     KVSQSTLS"
     gene            21487..22032
                     /gene="hpt"
                     /locus_tag="ACZPCL_02100"
     CDS             21487..22032
                     /gene="hpt"
                     /locus_tag="ACZPCL_02100"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR1161316.1"
                     /translation="MADKIRVLLTEEEVDKRINEVAEQINKDYAGKSVHLICILKGGV
                     FFTCELAKRLNMPVSMDFMSVSSYGAGTVSSGVVRIIKDLDEPLEGKNVLIVEDIIDS
                     GRTLAYLIEVLKQRGPKDIHLCTLLDKPERRVKKQVKVDYTCFTIPDEFVVGYGLDYD
                     QKYRNLPYIGVVEQDQEETQE"
     gene            22064..23905
                     /gene="ftsH"
                     /locus_tag="ACZPCL_02105"
     CDS             22064..23905
                     /gene="ftsH"
                     /locus_tag="ACZPCL_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1161317.1"
                     /translation="MKQQQTFRGFMFIILMLILLMLALRFPYARQAQTVTAQEFEQIL
                     GSGNVAEAVISPNPQTPSGYVTVTLANGQTVRQNVSDVKDAEQLLKDNSIDYSMEDVP
                     QENTLLTVVMPFMLSIVVVVVIVTMMNRGAAGGGSNARMMNFGRSRAKLSRDSKVNFT
                     NVAGLEEEKEELEEVVDFLKNPQKYTSVGARIPKGMLLVGPPGTGKTLLAKAVAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDLFEEAKKNAPCIVFIDEIDAVARRRGTGMGGGH
                     DEREQTLNQLLVEMDGFGVNEGIIVMAATNRVDILDPAILRPGRFDRKVAVGRPDVKG
                     REEILKVHSKEKPLSEDVDLRRVAQTTAGFTGADLENLMNEAAIISARDNRRFIRQAD
                     IDKAFVKVGIGAEKKSRVISEKDKKITAYHEAGHAILFHVLPDVGPVHTVSIIPTGVG
                     AAGYTMPLPEKDEMFNTKGKMLQNIMVDLGGRIAEEIIFKDVTTGASQDIKQASKLAR
                     AMVTQYGMSDRVGMIQYGSDEDEVFIGRDLAHTKSYGNEIADVIDEEVKRIVDECYTK
                     AKNIILEHEDVLHSCAALLIEKEKIGQEEFEALFKNHEPVMHNDGEM"
     gene            24202..26388
                     /locus_tag="ACZPCL_02110"
     CDS             24202..26388
                     /locus_tag="ACZPCL_02110"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIR1161318.1"
                     /translation="MDLIINIKEEKPVKNNIQINRDALFSAETQDYRMPAEPDIHDKV
                     VVRMRTAKDDVDHVYYIEGKKETEMKKTVSDTLFDYYEHTMTAGEEQILYHFKIVKGT
                     EVCQYNRLGVSDQDTDQFDFKITPGFHTPDWAKGAVMYQIFVDRFCNGDPTNDVQSCE
                     YVYIGKPVYQVKDWSRNPSTMDVGCFYGGDLQGVWDKLDYIKSLGVEVIYLNPVFVSP
                     SNHKYDCQDYEHIDPHFGKIVKDGGSMVDQNAADNTKSERYVVRSADRENLEASDAFF
                     AEFVKEVHNRGMKVILDGVFNHCGSFNKWLDAEQIYERSGDYEPGAYISKDSPYHSFF
                     HFHDNSDKAWPYNRTYDGWWGHDTLPKLNYEGSDKLVEYILNVAKKWVSPPYSVDGWR
                     LDVAADLGHTAEYNHAFWKKFRKAVKEANPQALILAEHYGDPSGWLQGDEWDSIMNYD
                     AFMEPVTWFLTGMEKHSDSYNGGLYGNGQSFFDAMAYHMSRMQTNTVFTAMNQLSNHD
                     HSRFLTRTNQVVGRIGSMGPERASENVKKCVMREAVMIQMTWPGAPTLYYGDEAGVCG
                     WTDPDSRRTYPWGREDLELMEFHRYMAGIHKRLPALRKGAVKPLFAANQQIAYGRMWD
                     RYQTVTVISNLPQPSAIEIPVWQLGITDEDIMGRPIITTEDGYNAGILFYHVKDGILK
                     VRMPAYSGAVFASHPEDFYPVLPSRFVEEGEEEAREKEQLEKAAEA"
     gene            complement(26535..26620)
                     /locus_tag="ACZPCL_02115"
     tRNA            complement(26535..26620)
                     /locus_tag="ACZPCL_02115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26584..26586),aa:Leu,seq:taa)
     gene            complement(26700..26781)
                     /locus_tag="ACZPCL_02120"
     tRNA            complement(26700..26781)
                     /locus_tag="ACZPCL_02120"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26745..26747),aa:Tyr,seq:gta)
BASE COUNT         7964 a         5281 c         6965 g         6911 t
ORIGIN      
        1 ctccactgcc tgctacgcag gcggaacctt cgttaatagc ccaagaaaaa caaatgccgc
       61 tcttggcggc gcttgttttt ctcttggact cattatatca tgaattgaaa gtgtggacaa
      121 tgtattgtaa acattcggtg aaatttttta aaaaaaatat taaaaaaatt gttgacaaga
      181 agaggatgat acgctataat tttcgttgtg ttcaagagaa cgcgccttta gctcagttgg
      241 tagagcagta gactcttaat ctatttgtcc agggttcgag tccctgaagg cgcattgaaa
      301 ggtctgattt tgcagatatt ggaggctgcg gggtcggact tttttgttgt ataaaattag
      361 atgaacaggc gcaatgagat tgtggctgga tcattatttc ttatggcagt gtttatggct
      421 gggacaggga ccgaggcatt tatttacctc gcgaaaagct ttgcaaagct ttaaagcccg
      481 tttgtcccag tcttttttgt cgcaggtggc tttgcagagc tgtcggatca gtttgggtgg
      541 gagtttcatg gaaatgaggc tccttttttg ttttgttatt tctatatata atatgtagga
      601 aaatgaaatg tggataaaag aagggaaaaa gagataatat gtggatgaat gtggataaaa
      661 tgagataaat ggatttaaaa tgcaaagatc ccggcggatt ggtatgccgg gacctgggtg
      721 catatacggt ggtttaattt tcttcgtttt ctttaggctc tctcatagcg atcagtttgc
      781 agataggatt tcccatagag gaaaactttt cttcatattc tgtcattaca tttccggcac
      841 acatgaccgg atcgtggtgc agatcgaagg tgtgggcggt aatggtccag ccagcttcag
      901 ggattgcttc cagggagtaa tcaaaaaggc ccttattgtc ggtcttaaat tctaccatac
      961 cgtcagctgc caggatcttg tcatatacgg ccataaaatc cggggaagtc agacggcgct
     1021 ttgcatggcg gtcttttggc catggatcgg agaagttaag gtagatcttg tctacttctc
     1081 ccggtgcaaa gtagtctgcc atgtttttgg catcgatgca catgaaataa atattgttta
     1141 attctaactg ggcacgtttt tgaagtcctc ttaaaagtac gctggaatag cgttcaatgc
     1201 cgatgtagtt gatatcaggg ttctgctgtg ccagttccat gataaaacgt ccttttccca
     1261 tacctacttc gatctggatg gggttgtcgt tgccaaaaac ctggttccag ctgcctttgt
     1321 gaagctctgg ttcctggatc acataggggc tggtagcaat agtctcttct gccccgcgta
     1381 tatgtcttaa acgcatggat attgtcctct ttcttaatat ggtgaaaagt atcttccgca
     1441 actgcggaaa atacggatat atggtgaaaa tatatctgct agtaactata tcatatagtg
     1501 cttagtgatt gcaaggtgaa ggaaaattta gtatacttac gttagcagat aaaagagcag
     1561 tcgggagtga aaacaagggt gttgcaagct tgcttgcata agcgcttgct ttcgtttccg
     1621 aatattcggg cagcgcccga agcttcgtgt tttgccaaaa acacgaagac tgctcttttt
     1681 aagtttatga ataagtgatt tcggggggaa aattacatac aaaggaggaa gggcgatgtc
     1741 aaactttgaa cgtactacca gctggcagca gatccagcag ggggtgaagg aagcggagcg
     1801 cctgatcgtc agtaaagatt acaatcttgt tatggtgaag gccagacaga cgttggagtg
     1861 tatggtccga tgtctggctg agaaggcgtg tcttgtagat ggagatctgg cagataccat
     1921 agatcagctg tatgagggac attggatcga caagacgacc aaggatgatt atcataccat
     1981 ccgtattctg ggaaataagg cggtccatga aaacgataat acggcctata atgcaaatca
     2041 ggcatatcag cttctaacca aacaggttta tgcgttttct catgattttc aggtggcaaa
     2101 gacctttaca gtaccggtaa cagatacaag accggtgcga aatgccagaa caggtgcaac
     2161 cactggcagt tcaggtgcag gaagtgcatt ctatggtcag acaggcgggg caaggccgcg
     2221 tacaggtacc ggtgcagggc agactgcagc ttcaagagca cagtcatctg taagaagtca
     2281 gacatccccg agatcccaga catcagccag aacctctaac ggtacaggaa atggaccgcg
     2341 ctccggtgca gaactgcagg gactttccag tcgttccgga gcaaacggaa gccggagaac
     2401 ggcaggaagc aatagcagca caggcagcag aaacagaaat tcccaaagta ccaaccgctc
     2461 ctacagcgga tcttcccgca gcggaaggac tcgcagaaga aaacacagac catcacctgt
     2521 ggcaatggta ttaaaattcc tgatcccgat ccttgtgatc gtagtcctgg tagtagcgat
     2581 ccacactctg gtgccgggaa agaagaaagc agatccgacc cagccggaga ccatgtccac
     2641 agaaatgaca tcagcagaga cagaagcacc gactccggaa gagacccagc caccggctga
     2701 tgtatatgta gttacaggaa ataaagtaaa tgtacgtaca gaaccgtcta ccaatggccg
     2761 tattttagta cagctggaag gcggaacaga agttacgtat gtaaagcgtt ataataatga
     2821 ctggactgtg atcaattatg atggtcagga agcttatgtt tccagccagt atatagcaaa
     2881 acaggaagca gaaacaactg cagagaccca ggcagaagca gaaacagccg ctgaaggcga
     2941 gactgcagca aaccagtagt gtcgctggtt tggtcagtgt atggctgagt tattgagaac
     3001 tttgtgcaaa acaggagaat gagacccata aaaaggtccc attctccttg tttttgtgca
     3061 taacgcttaa aaatttcgtt aaatttttaa tttatgtaca atttcgctct gttaattttt
     3121 gaaaaaagta gtatgataaa aaaatgcaaa aagcaaattg cattaaaaaa agaacaaaga
     3181 aagggaggac tgtgacatgg atacagtgat caaccggctg tctgatattg agaaagccgc
     3241 cgtttctgtg atggatggtg ccagtgagcg aaaaaagcag atggcccgtg aaatggaaga
     3301 gaaaacggcg gcttttgatg cacagctgga aaaagaaaac caggatcgca tttcccagat
     3361 acgcagcaaa atggaagagg aattacagca ggagttaaga caacaatcag aggatgccaa
     3421 agctacgatg aagcgcctgg aggccaccta cgaaaaccgg catgaggaat atgcgcaggc
     3481 tctctttaaa aatatgataa aggagtgaga acatgggaag cctgatcagt tacagcggca
     3541 tttccaccaa ggtgaaggct atggagcgtt tcaggatccg cccggagcaa tttgaggcaa
     3601 tggcagacct gggatctgtg cctgaggcgg ttcagttctt aaggagtaat ccctcttatg
     3661 aaaaggcatt ttccaaagct tctgaggatg agcttcaccg cggaaagatc gagcagctgt
     3721 taaacttaag caaataccat gattttgcaa agctgtaccg gtttgcaaat gtagggcaga
     3781 ggcgttttct ggacctttat ttcatgcact atgagatcga tatattaaaa acctgtctca
     3841 gaaatgcagc gggaaacaga gaaagtgccc aggatcttac ggggtttaag gaattttttg
     3901 ataaacattc ggatatggat ctggtgcagc tgtcccagtg caggagcata gaagatgtga
     3961 tccgcgggtt aaaaggttct gcttactatg agccattaga aaagctttta gaacagggaa
     4021 aagcaacgct tccggcctgt gaaacggcta tggatatgct ttatttcaag actatctgga
     4081 agataaagga taaatatctt tcaaaggcgg aggcaaaaat gctggcccag tgctttggca
     4141 cgaaaatgga tatgttgaat tttcagtgga tctgcagggc gaaaaaatat tataacctgt
     4201 cggaaggtga tatttatgcg ctgatcattc cgatccagta tcatgttacc aaaaaagaga
     4261 tccagaccat ggctcaggcg gaaaacatgg aacaggttta cggggtgatc aaaaacagct
     4321 ggtacaaatg ccagaatata gaagagctga tggatcaggg ccttggaaat gcggcaaagg
     4381 agcttacaga taagatctat gagcttacca gcaggaaaga gccttattca gcagcagtat
     4441 taaactctta tctttatttt aaagaaagag agatcgagca gatcataact accatcgaaa
     4501 agatacgtta tgggatgaca gcaaggacat aagggggtta atgtgaagtg atagagaaga
     4561 tgaagttctt aagcattacc ggccccaagg cagacattga ccgggtggtg gatacctatc
     4621 tttccagata tgagatccat ctggaaaatg ccctgtcaga gttaaagacg gtaaaagact
     4681 taagacctta tattgagacc aatccatata aggaaaaatt gaatattgcc agagaactgg
     4741 cagaaaagat cggcggacag ctgccagagg tttcagagac ggaacttcct acacaggaag
     4801 aagcagcaaa gatggtagat gatctgaaca gccggttaaa ggaattaaat gataagaaag
     4861 aagagcttca ggcgcagatc cagactttaa agcgctctaa agaccaggta gcaccattta
     4921 cagagttaaa ttacagcgta aaagagatcc tgggcttcca gttcatcaag ttccgctttg
     4981 gccgtatttc cagggaatac tttgacaagt tcaacagcta tgtgtacgag accattgaca
     5041 cggtgatgtt taaatgcctg gaagatgcgg aatatgtctg gatcgtctat ttcgtgccgg
     5101 agaagctggc agacaagatc gatgccattt acgcgtccat gcattttgaa cggtgtttcc
     5161 tgccggatga gtatgaaggt actccgatgg aagcggaaca tgtgttagat gacaggatca
     5221 aggcgttaga ggctgaaaaa caggaagtgg aaggaaagat ccttcagaca ctggatgaaa
     5281 ataaaaaaga gctggcagca gcccgtacca gactggagcg cttctccgtc aactttgatg
     5341 tgcgtaaaat ggcagcctgc accaagcatg gagatcatac tttctacatt ctctgcggct
     5401 ggatgagcga aacagacgca aagaagttcc agaaagagat cgagtctgac gcagatactt
     5461 tctgcatcat ggaagataac cacaacaaca ttatgagcac tccgcctacg aaaatgaaaa
     5521 atccggggct gtttaagcca tttgaaatgt atgtggagat gtatggtctg ccttcttata
     5581 atgagatcga tcctacgatc ctcatcggta tcacctattc tattttattt ggatttatgt
     5641 ttggtgatgc gggacaggga ctctgccttc tcattggtgg atttttactg tataagttca
     5701 aaaaaatgcg tctggcaggc attatttcct gctgtggctt cttctctacc atattcggcg
     5761 tgctgttcgg cagtgtgttc ggatttgagg atatcattga cgcagtgtgg ttaagacctc
     5821 aggaagccat gacagatctt ccatttattg gaaaattaaa cactgtattt gtagttgcta
     5881 ttgccatcgg tatgggcatt atcctgttat gcatgatctt aaatatcatc aacagcgtaa
     5941 aggagcacaa cacagagaaa acatggtttg acaccaacgg cgctgcggga ctggtatttt
     6001 actttgcact ggcaactgtc attgtcctgt atatgagcgg aaatgcgctt ccggcgacta
     6061 ttgtactgac tgtattattt attatcccgt tactgcttat gttctttaag gaaccgttgt
     6121 cagcaatggt agagaagaag gcacagaaaa tggaaggcgg cgtgggaatg ttcattaccc
     6181 agggcgtatt tgaactgttt gaagtgcttt taagctattt ttccaatacc ctttctttcg
     6241 tccgtgtagg cgctttcgca gtgagccatg cagccatgat gcaggtggtt ttaatgcttg
     6301 caggcgcaga ggccggaagt cctaactggg cggttgttat cgggggaaac ctgtttgtat
     6361 gcggtatgga aggcctgatc gtaggtatcc aggtacttcg tctggaatat tatgagctgt
     6421 tcagccggtt ctacagagga agcggacgag catttgagcc gtatggcaag cattagcaga
     6481 atgaagagaa aaaggagaaa tcaatcatga gtttattagt taaaatgaca ttaagcgcag
     6541 cattagtatt aagcatcggt gtaccttttg gagcatttgc agcaggagaa aagacaaagg
     6601 gacgttataa aacagctctt ggcgtaaacg tatttttatt cttcggtacc atgatcgcag
     6661 cagcagcttt catgttttca ggaaatgcat ttgcagcaga ggaaacagca gcagccgctt
     6721 ctaacagcgc agccggaatg ggctatctgt cagcggctct tgcaaccggt ctttcctgtt
     6781 taggcggtgg tattgcagta tctgcagcag caagtgcagc actgggcgct atcagtgaag
     6841 acagctctat tcttggtaaa tcccttatct tcgtaggtct tgccgaaggt gtgtgcctgt
     6901 atggtctgat catttccttt atgatcttag gtaagcttta atatgaaaat gtatctcatc
     6961 agcgataaca tcgacacctg gaccggcatg cgcctggcag gtgttgacgg ggctgtagta
     7021 catgagaggg acgaactgaa acgtgagctg gataaggttc tggccgataa atccattggt
     7081 attatcctgt tgactgaaaa gtttggcagg gaatttccgg agatcatcga caatgtaaag
     7141 ctggagcgga agctgccgct gatcgtagag atcccggacc gccacggaac cggccgcaag
     7201 ccggacttta tcacctctta tgtaaacgag gccattggac tgaaactata gaaagcaggt
     7261 gaagagcttg acaacagagg aaaagctgaa gcattttcag gagatctgta tgacagatgc
     7321 aagggaaaaa agtgcaaaaa tactggacga ttacgcaaaa actcttgata aggcctatga
     7381 ggagcataca gaggatgcca ggaagcgggc gaaaatgcag gaagaggctg agacggaaaa
     7441 gttagggcgt gagcgcaaca aaaagctttc catcggacag ctggacttaa gaagagaagt
     7501 aagccgcaga caggaagaat taaaggataa actgtttgta gaagtgagag ataaactggc
     7561 gaatttcatg gaaaccagag agtatctgga tctgttggaa aaacagatcg gggcagctaa
     7621 aaaggttgcc ggtgatgagg ccatgatcgt gtatatggat ccttcagatg aggataaggc
     7681 ccgcagactg gccatgcacc acaatgtaac agtgaagatc agtgaatatt cctttgacgg
     7741 cggtatccgc gcagtgatcc cgtccaaaca tatcctgatc gacagttcct ttaagacaaa
     7801 actggaagaa gcgcgccatg aatttaagtt tgacttggga ggtaagcagt aaatggctaa
     7861 gacaggtgtg atctacggta tcaacggacc tgtagtttct ctggccggtg atcccggatt
     7921 tcagatgaac gaaatggtgt acgttggtaa ggaaaatctg gtaggcgaag tcattgggct
     7981 tacctcagaa aaaacaacgg tccaggtgta tgaggagacc agcggtttaa agcctggtga
     8041 ggtggtaaca ggaacaggag caccggtttc tgttacactg gcccctggta ttttagataa
     8101 catttttgat ggtattgagc gtccgttaag caagatcgca gagcgcagcg gctactatat
     8161 cagccgtggc atcagcgtag attctctgga tacttccaaa aaatggaaaa cacatatgac
     8221 agtaaagaaa ggcgaccggg tgcttccggg aaccattatc gcagaggttc ctgagacaag
     8281 ggctattatc cataagataa tggtgccgcc ggatacagag ggctatgtat tagacgtagt
     8341 ggaagatggg gaatatacca ttaatgagcc tcttcttacg ctgcagttac ttgatggaac
     8401 agaaaagaag ctgaccatga cccagaaatg gcctatccgt gtgccgagac cttctgcaaa
     8461 gcgttatccg gcaaccagac ctctgatcac cggacagcgt atcctggata ccatgttccc
     8521 actggcaaag ggaggtacag cggcaattcc aggaggtttt ggtacaggaa agaccatgac
     8581 ccagcatcag atcgcaaaat ggtctgatgc ggatgttatt atctacatcg gctgtggtga
     8641 gcgtggaaat gaaatgaccc aggttctgga agaattttca gagctgatcg atccaaagag
     8701 cggaaatccc ctgatggaca gaactacatt gatcgcaaat acctccaaca tgcctgttgc
     8761 agcccgtgag gcaagtctgt atgccggtgt aacattggca gaatattaca gggatatggg
     8821 atatcatgta gcaatcatgg cagactctac ttcccgttgg gctgaggcgc tacgtgagct
     8881 ttccggccgt ctggaagaga tgccggcaga ggaaggtttc ccggcttatc tggcatcccg
     8941 tctgtcttct ttctacgaga gagcaggcat gatgcgcaat ttaaatggta cagaaggttc
     9001 cgttaccatt atcggtgcgg tttcccctca gggcggtgat ttctctgagc ctgttaccca
     9061 gaataccaaa cgttttgtac gctgcttctg gggactggat aagaaccttt cttatgcaag
     9121 gcatttcccg gcgatccact ggctgaccag ttattcagag tatgtaaatg acctgtcatc
     9181 ctggtatatt gataacgtag acaagaactt tgtaagtgac agaaaccgcc tgatggcgtt
     9241 attgacccag gaaagcagtt tgatggagat cgtaaagctg atcggtgcag atgttctccc
     9301 tgatgaccag aaactggtct tagagatcgc aagggtcatc cgtgtaggtt tccttcagca
     9361 gaatgcattc cacaaggatg atacttccgt accccttacc aagcagttta agatgatgga
     9421 gaccatttta tatctgtaca agaagtcaaa ggcactgatc gccatgggaa tgcctatgtc
     9481 cgtgttaaaa gaggacaaga tctttgataa gatcatttct attaagtatg atgtgccaaa
     9541 tgaccgtttg gacatgtttg atgattacaa gaaacagatt gacgcgttct atgagcatgt
     9601 gctggagcgc aacgcataag gaggacttaa aatggcagtt gaatatttag gtttaagtga
     9661 gatcaatggt cctctggtag tgttagaggg cgttcagggc gcagcttatg atgaaatcgt
     9721 ggaaatgacc gttggcggca ctacaaagaa gatcggacgt attattgaga tctacgggga
     9781 taaagccatt atccaggtat ttgagggaac agacggtatg gcccttagaa atacccatac
     9841 aaggctgaca ggacatccta tggagatcgc cgtttcagag gaaatgttag gacgtacctt
     9901 taatggtatc ggacagccta ttgacggact gggggatatt atttctgata tccgcatgga
     9961 cattaacgga cagcccttaa acccggtatc ccgtgagtat ccaagaaact acatccgtac
    10021 cggaatttcc gccattgacg gactgactac actgatccgc ggtcagaagc ttcctatttt
    10081 ctcaggaaat ggccttcctc atgaccagct ggcggcccag atcgtgcagc aggcatcatt
    10141 aggtgataat tctgatgaaa agtttgccat cgtttttggc gctatgggcg taaagcatga
    10201 tgtggctgat ttcttccgcc gtacctttga ggaaagcggt gtttccgaac atgtagccat
    10261 gtttatcaat ctggcaaatg acccggtagt agagaggctt atcacgccaa agatcgcatt
    10321 gacactggca gagtatctgg cttatgaaaa gggaatgcac atcctggtca ttttaacaga
    10381 tatgacttct tatgcagagg cattgcgtga ggtttcttcc tctaagggcg agattccttc
    10441 acgaaaaggc ttcccaggtt atctgtacag tgatctggca tccttatatg aaagagccgg
    10501 tatcgtagca ggaagaaatg gatctgttac ccagatcccg atcttaacta tgccaaatga
    10561 tgatattaca cacccgatcc cggacctgac cggttatatt acagagggac agatcgttct
    10621 ggaccgtcag ttaaacagcc agtctgttta tccgccgatc agcgtactcc catctctttc
    10681 acgactgatg aaggacggta tcggtgaagg atatacaaga aaagaccacc aggatgtggc
    10741 aaaccagctg ttttcctgct atgccaaggt aggtgacgcc agggcgctgg catctgttat
    10801 cggtgaagat gagctgtctg ccattgacaa gaagtatctg gagtttggta aagcatttga
    10861 ggagcatttt attggccagg cacatcatga gaaccgctcc atcatagaga ctctggagat
    10921 cggctggaaa cttttacgta tgcttccaag agaggaatta gaccgtattg ataccaaggt
    10981 tctggatcaa tactacgagg agtgaggtga gcaggaatgg atccaaatac atttcccaca
    11041 aaaggtaatc tgatcgctgc gaaaaactcc ctgaaactgg caactatggg ctacggatta
    11101 atggataaga agagaaatat cctgatccgt gagcttatgg ggctgatcga tgaggcaaag
    11161 gggatacagt ctgagatcga cactgtgttt aaggctgctt atgaagcact gcagagcgcc
    11221 aatattgagc tgggcatcaa ttatgtccag gagatcggaa tgtccgttcc tgtggacgat
    11281 acggtaaaga tcaaggcaag aagtatcatg ggaacggaaa tccctctggt gcagcatcag
    11341 gaaaagccta tggagctggc ttatgggttt aataatacct cggaggcgtt agacattgcc
    11401 agagagcggt ttgaacgggt aaaggagctg accattaagc tgtctatggt ggaaaactct
    11461 gcataccggc tggcaaattc tattaagaag actcagaagc gtgccaatgc gttgaagaac
    11521 attaccattc ctcattatga agagctgtcc aagtctattt ccaatgctct ggaagagaag
    11581 gaaagggaag aatttacccg tctgaaagtc attaagagga tgaagaccgg gtgatggaaa
    11641 taataaaatg gagaagctga caaagcgcct gaatggattg aaataggggg atactgtatc
    11701 atgtatacat ttaaagatct ggtagagatc accgcgaaat tacgggcaga agatggctgt
    11761 ccctgggacc gggaacagac ctttgaaagc ctgcgaaaat atatgcggga agaggcagag
    11821 gaagcagttc aggcagtgga taaaaatgac atggaaaacc tttgtgaaga actgggagat
    11881 gtgctctttc aggtgatgtt aaacagccag atcgcaaagg aaaaagggct gttttctata
    11941 gatgatgtga taggtgggat ctgcgaaaaa atggtgcgca gacacccctg ggtattcggt
    12001 ggtgaaaaga taaattcgac cgaagaagga gaggctctct gggcccggat aaaaatgcag
    12061 gaaaaggcca aaaaaccttg acaaattata gtaaaacaga tataaatgat agggtatcac
    12121 agacaagaac gaaaacggtc cgaacctgtt ttttttcgga ttcggtttag gaggaaaatt
    12181 tgatgaacaa gacagagtta atcgcagcag tagctgagaa ggcagaaatt tctaaaaagg
    12241 atgcagaaaa ggcagttaag gcttttactg atgcagtagc agaagaactg gcaaagggcg
    12301 gaaaggttca gttagttgga tttggtaact ttgaagttag cgaaagacca gcaagagagg
    12361 gaagaaaccc aagaaccggc gagactatga ccatcgctgc ttccaagaca cctaagttca
    12421 aaccaggtaa ggctttaaag gacgagatca acaaataatt gattttataa gaaaattcgg
    12481 taatcccctc cgtgaacatt tgcatcatgg aggggatttt gtattaaaag gagcgacttt
    12541 atgagattag ataaatacct gaaagtatcc cgtatcatca agagaagaac cgtggccaat
    12601 gaagcctgtg acagcggtcg ggttatgctt aatgacaaag tagcaagagc cagtgcagag
    12661 gttaaagtag gtgatgtgat cgaaattgcc tttggtacca aggctgtgaa agtgaaggtt
    12721 acaagcgtac aggatgccat ccgcaaggaa gatgccaaag agatgtttga gtatctgtaa
    12781 aaacagacca ataaatagac ttcgtcaggg aattctgtga aagttacagg atcctctggc
    12841 gatttttagt tgcctgaaaa aattttttaa tctataatac aaatagagtg tcaactcagt
    12901 tgtgtagcaa aactttaaaa tgtgtttctg atctgaaagg ttgggaatgc attaaaaaca
    12961 tggagaacga tatggcaaga aatacggaac gtgacgagag agaagccacg cgcagaaaag
    13021 aacagctgat gaaagctgga tttaaactgt tttcagagaa tgggatcgaa aatgtaagtt
    13081 tgcagaaggt ggcagatgca gcggatgtag gggttgccac tatgtacaaa tattaccaga
    13141 caaaggtaaa actggtagta gccatcagtg gaaagatatg gggggatatc tggaaaagtg
    13201 tgctggccca gaatggaccg gatttctttg aaaactttac agcttatgag tatattgaat
    13261 tttatctgga catgatcatc cggttataca gggaaaaacc ggaaatgctc cggttcagca
    13321 ataattataa gaactttatt taccatgaag gaattgaaga agagccttta ggggaacacc
    13381 tggacgtgtt ggaaccttta agagccatgt atcacaagct ttatgaacag gcaaaagtgg
    13441 atcacagtat ccgcaccgat ctttcagagc aggagctttt cacaactgta gccattgcca
    13501 tgctggcggt ggcagagcgc tatgcccagg ggatcgtatg ggccaatgat cataaaacgg
    13561 accataccca ggagttaaaa tatctgaaag aaatgcttct tatgtggtgt aagggaaaaa
    13621 cggaataaaa gaagacagga atagaagatg ggatctggtt tggggaaatt tttccccagc
    13681 ttctttgcta tgatctggta acagagatag caaaggagga ttttcttatg aatacactgt
    13741 ttaccagccg tttaaatggg atctttattt taaacacatt gttggagctg cataagccgg
    13801 ttacagtgtc tgaactggca gggatcatta accatcgcta tgatcatgta acagctcccc
    13861 ggaaaatggt ttctgcagat acggtaaagc ggtttctcaa cgatttttac cagaccactt
    13921 ccgggaccga ttactgcagc atccggcttt tctgttttaa gaaaaaccgt tatggcagtc
    13981 tggtcccgga tcatgatctg gaaaatgtga ctttagctac ttatgtagct gcctgtctgg
    14041 aacttaatcc ttcgatgaac gtgcgagacg gaaggggatt aaaatccaat tctgttgtgc
    14101 actttcacac ggcctgagat ctgtttgttt tcttcccttg cccgccggat cgcgccttca
    14161 atgtcgctga tccggcattg ttttatggct ttttcgttaa tggaattttg aagacctccg
    14221 tttgcatcgc ccatgatccg ttttgccatc tggacagaaa ctttttcaaa taaggctctg
    14281 gcttcacgac cgtttccaaa gttttcatca tttacacggg ttgagaaata agcagtgatc
    14341 ggttctttcc agttttccgg cagttcgtat ccactcattt ccgcatgttt cttaaagatc
    14401 tccgccatct ccggggctgt ataggacgga aaatagaaag tggagttaat acggctcttg
    14461 ataccggggt tggcatcgat aaattctttc atcttgttgt ttttttcatc tacatccgtt
    14521 ccgccatatc cggcaaagat caccagttta tcttctgcat gttcttctaa ctcgatcact
    14581 aactgggcaa tggcttctct gctgaaggag tccggttcgc ttcggtcacc ggtgagagaa
    14641 taagcttcat caataacgat aatgtcattt tcctcaaata aagctttggt tttcggggcg
    14701 gaatggccta catacatgcc ctttaattca gcaccgttta cgcaggtgaa ttgacttcca
    14761 ggcagaagtt tttcttcttc catgatcttt cccattaatt tagctactgt ggttttggca
    14821 gttccgggag caccaagcat catgtgaacg ttgtggtaac tgctcttttt catgcccatt
    14881 tccttgcgca gacggttgaa tttcatgacc tggacggttt ccatgacctg ttcttttacc
    14941 tggtccatgc cgatcagatc ttctcttaaa cagcggatgg cagaacgggt ctctgtcttc
    15001 tgctctgcag gtccccgttt gcctgcaaaa cgttccataa actgaaaatc cttttttcgc
    15061 aggataccat gttccttgtt ccgcagggca tattttagga tcttatctaa aaagtcatag
    15121 gaaaactctt tattcatttt ttcaatatcc agtaaaaaat catggaccgg aaatttacgt
    15181 tccagcttgc agccgctgtt tgtgaccatt tgtttccaga tattttcata gtagagagag
    15241 acagagcttg tttttggtat ttccagctct tctgcggtaa ggcttaatat gatctggttc
    15301 aggtttttat cctcatttga ctcaaaggtg tcaaatccgc caaatcctcc aaaagagtta
    15361 aaggagtcga agtcattttc ttcgtgaagg atcaggacgt agacctgttc attgctgcgg
    15421 aagttattta aggtatcaat gatcgggtca cacctgaaaa aactgatatt ctccatagag
    15481 atacagacag gtttatcctc attttctgcc cagtaaggaa caaaggtgtc atttgtcttc
    15541 tggctggcca gaaaggagtt tccaaaagag gagaagtcat tttcctgcca gttgtaatag
    15601 tcatcggctt ctttaaatgg aataatgggg aggcattgcc agctgtctga ccactggatg
    15661 tcaggctctg ccccttcttc ccactctcta cgactgtgaa tctcattgta gatacctgct
    15721 aaaaggctga tagccaggta tccttcctcc agtttggaag cgtgtaccag gatatactgt
    15781 ggttcctggt gatcctttac aaatttctgg atttctttaa actgcactcc cccgtctttt
    15841 atgatctgtg aaagtccttc tttacagaac tctttggata tatataaata ctctgctttc
    15901 tggggattga ctttgcccag attttgtttt tgaagttttg gtacgatcag gttctttttt
    15961 tcgctcatag gatcagctcc tttatctgta tctgttttca ggtttttaag gttgttgtct
    16021 tgttgatcac gcccttacta tagcggatca ggctatgcaa aattgcctga ttttgaaaaa
    16081 atttattttt tcctgtatac ttgtgaaaga aaatggaaat gagagtacat aaaataagat
    16141 tacataaaaa tgcaaaagga gatcacagac atatggcttc ccggtacaat cagctgacta
    16201 tgctttatat ttttaatatt ctgaaactgg atgcttctcc gaaaaagccc tattcggcac
    16261 ctatggtcta tcgccgttta aaggaactgg ggatcgatct ggaccggaag acggttttta
    16321 atcatatgaa gatccttaca gaccagatgc tgtctgcgga ccaggatgaa gagtcttttt
    16381 tcaaacagta tctgtgcagc aggctgtttc gctgccggaa agaggaaggg aaagaaaaaa
    16441 gcagggaaaa tggcagaaag cccggaaatg aaagcagcta ccggatctgt ctgccggaag
    16501 aggaaatccc ggaaaacagt gttttatatt attatataga agatgaaatg tcaccggagg
    16561 agctggaact gcttcaaagt accgttgcta ttaaccagta cctttcagcg gaaaaaacaa
    16621 gggagatcct ggctgatctg gagcataaaa agccttttga ctgcagggaa cagtctcttt
    16681 atacagcatt tgcggacccc aggctgaaaa accaggcaac ggatattttc gccagtttgt
    16741 cagtaatacg ggacagtatc cgcaagaaga aaaaactccg gatcctatat ggacggtatg
    16801 acagaaatct ggaactggcg cctaccagtg aagatgcacg gaccattgaa ccttatgcag
    16861 tgatgtcaag taacggttat tattatctca tagcaggaaa tcccaaatat cccgatggac
    16921 tgaccaattt ccgcattgac cgtattttac agatccagat gttggatgag cccagaaaac
    16981 cattgccgag cgcccttctt ccctatttcc gggatgcagc ccacgaagtt tttcaggcca
    17041 gcgagtaccg caatgaccat ccggtcatgt attcagacga aacagtgcgg atccatttaa
    17101 gctgtgatcc ggtgatctta aataatctgg tggatgattt tggctggagc atccagacac
    17161 gggatataaa agatccggac catccagact gggtccatgt aactgcaaaa gcaagcctga
    17221 tgggggcagc tgttttctgt acccatcact gtaggcactg caaggtgatc agccctcaaa
    17281 aactgcggga aaaagtggtt gaaaacctga aagcagggct ggctctttac gaataaaaac
    17341 ttaaaagtca tagaatgtgt ttccacccca tatatttaac tgatacatac tctgaaagct
    17401 cagagcaggt taatgagtga atatatgggg gtaattttta tggaagaaaa aatatcatcc
    17461 ggcaggcttc accggctggt gctttcggac aggcatacag gttctgtaac aggagtcaat
    17521 gatgtggtgt cttttgatga aaatgagatc gtgctggata cggatatggg ccttttgact
    17581 gtcaggggaa aggaacttca tgtaaagcgg cttacactgg aaaaggggga gctggatctg
    17641 gaaggacagg tggacagttt gaattacagt tccaatgcag cactgaaacg gtcgggagac
    17701 tcatttctta accgtctttt taaataatcc tggaggtgat ttatgagcat gcagatccgt
    17761 tttgaagcct ggctgctact tttgagcctg ttggtgggct gctggctgat gctggtgtat
    17821 gacctgctgc gggtattgcg gcttatgata aaacacagct cttttgtaat ggggatagaa
    17881 gacttttttt actggatcta tgcggggaca gccactttta tgctgctgta tgagctaaat
    17941 gacggcggag tcaggatgta tgcagttgcg ggcgtctttg cggggatgat cctttatgat
    18001 aaaattgtca gtcggtttct ctttgcagcc ttgaaaaagg ctggtaaatg gtttaaaata
    18061 aaaagaaata gataaaaaag atggtgactg atacatgagc tcctacggaa gatcaaaacg
    18121 cgtaaagcgg gaaaaatggg caaacagaat ggctgtactg ggcgttacaa tggtagttct
    18181 ctgtcttgca gttgctgtaa atgccaaggg ctcaactttg aagaagtctg acctggatta
    18241 tcagcaaaag gagaagcagc tggaagaaca ggtacgggaa gaagaagaac ggacgaaaaa
    18301 gctggaagag tacaaggtat atgtaaagac aaagcagttt gcagaagaag ttgcaaagga
    18361 gaaactgggt ctggtaaatc cggatgagat ccttttaaag cccaatcagt aaaatgagtt
    18421 tcggaataaa tgaccggcag aaaatgtcga aacattttct ggaagaaaca tgaaaatatg
    18481 acagatattt ccctcctggg tgcgtatact ggatgttgcg cagattagga gggatttttg
    18541 tgttgtcagg caaaaatgaa aatgggatca attattatac agccaggaaa ttaaaagata
    18601 tggcacagtc attggaacag ctggcaaagg cttttgatga aggtgccggt gagacaatga
    18661 accttacaaa ggatgatggt ctggctgcca tgcagacctg cgctgcactg gtctgcggta
    18721 actgtggcgg atgcagcctt tatgcagaca gtgaaaaaga agacagttat tatctgtatt
    18781 atcttttgcg tatatttgaa cagaacgggc ggatcggcac agaggatatg ccggagcttt
    18841 ttaagaagga ctgccgcagg aaacgggatt acttagagca gttaaaccgc agttttacca
    18901 gggcttccat gaatttgtcc tggaagaacc gctttctgga aagcagggat gtggtcatat
    18961 cccagttccg tgagctggca gtgatactgg aagaattttc caggcagatc gatgaagcaa
    19021 aaaatattac agaggaatat aaaggagcag ttaaaaaagc atttcgcagg tatcatatcc
    19081 tggtggacag tatgcttttg ctggaatatg aaagcgggcg cagggagatc tatcttacag
    19141 caaagaccac aaacggacgc tgtgttactt ccaaggatgc ggcagagctg atggagagcg
    19201 tttttcctga taccacctgg cttccggcaa aggacagtcg cagtatcatc acacgcaggt
    19261 atgaaacagt gcggtttgaa gaaaaaggag attattatct tacctggggt gctgcgggag
    19321 ttcccaaaca gggagaacgc tactcgggcg ataattacag tttttgtgag cgaggcagct
    19381 gtcaggtaat gttcagcctg tgtgacggaa tgggaagcgg ggcagtggca ggacaggaaa
    19441 gccggcggat cgtggaactt ttagaggcgc ttttggaagc cggttttgcc cccagatccg
    19501 cttttaaact ggtaaataca gtactccttt tagcaggcgg agaacaacac ccggcagcgt
    19561 tggacgcagg ctgtattgat ctgtatacag gcgccctgga agtattcaaa ctgggggctg
    19621 ctcctacttt tgtaatggga gaagaaggca tacaggtgtt aaaagcaggc caggtaccgg
    19681 caggcgcttt agggcaggca gaaccggtcc ttctttcaca gaaattatgg gacggggaca
    19741 ggcttgttat ggtgacggac ggtgttttag acgcccttcc gggagaagat aaggaacagg
    19801 tcatggggca gtttttagaa agtctggagg aaatgccgcc tcaggatatg gcagagcgga
    19861 tcctggagtt tgccctttct tttgttcccg gggcaaggga tgatatgaca gttcttactg
    19921 cgcaggtgtg gaaacggtaa tattgcatat gcaatgcaag aacagcggca ggataaggtt
    19981 gtgtaacaaa aataaagagc cccttgcgta tggggaagga tgtgggtata gtaagagtgg
    20041 acatgatctt taagggtcat gtaagcaaga cagaaacgga aatgaacata ctatggagaa
    20101 actggagtat acagtcaggc agacgatctg ccggtatcat atgctgcagc ccggtgatca
    20161 ggtggtcatc gcgctgtccg gaggggcaga ctctgtctgc cttttaagtc tgcttgcgga
    20221 tatgagagag gaaatggggc tgaaactgag gagtcttcat gtccatcacg gactgcgggg
    20281 agaagaagcg gaccgtgatg gggatttttc cataaagatc agtgaagctt tgggtgtccc
    20341 ctgtcatatg ataaaagcag aggtaaaaaa atatgcatcc cagacgggga tgtcggaaga
    20401 agaagccgga aggatcctgc gttataaagc cctggaggaa gaagcagggg cctgggaagg
    20461 agaaaacggt cagacagtaa agatcgcagt ggctcatcac agcagggatc aggcggagac
    20521 catactttta aacctgtttc ggggtagcgg tcttggcggt ttaaaaggga tcccctatgt
    20581 gagagatcgt attatacgcc ctatgttgga tgtttccaaa gaagatatca tcccttatct
    20641 tgaaaaaaaa cggttaaaat gggtcaatga ttccagtaat gacacagacc attatgccag
    20701 aaaccgtgtc cgttcccaca ttcttccggc gatcaaagaa gaaatcaatc cgggagcaga
    20761 ggcaggaata cgccgtgccg ggtatctggc aggaatggca gatgtgtatc tgaaaaaaca
    20821 ggcagatcag tgggtgcaaa aatatgtaaa agaggaagag catacctgga tgatcccgga
    20881 aggacctttt aaagaggcag atccggtgat ccgcctttac agtgttatgg aacttttaaa
    20941 aacagcagcc gggtgtgcaa aggacctggg actggtccat gcagaacagg tagaaaaact
    21001 tgttgacctt cagaccgggc ggaaggtgtc tcttccttat ggactggaag ctcgaaaagt
    21061 atacggcggg atcagcctcc aggcagtaaa aaaggagcct ttggaggaaa gaataaaaaa
    21121 tccccttccg gagctttctt ttgaggtttt ttcttatgaa aaagcaatgg aatttcccaa
    21181 aaagacgtat acgaaatggt tcgactgtga taaaataaag ggtacgcccg ttgtcagaac
    21241 caggcagacc ggggattata tcaccctgga aaatggcagt cgcaaaccat taagacgctt
    21301 tatgatcgac gaaaaaatac ctgcagataa aagggatgaa atagccctgt tagcagatgg
    21361 ggaccatatc atgtggatca taggcaggcg gatcagcagt tattataaaa taggccctga
    21421 tacacggcgt gtattacagg cgaaagtttc ccaaagtaca ttatcataga aaacggagga
    21481 taaatcatgg cagacaaaat tcgtgtatta ttaacagaag aagaagtaga taagcggatc
    21541 aatgaagtag cagagcagat caacaaagat tatgcaggca agagcgttca cctgatctgt
    21601 attttaaagg gtggtgtatt ttttacctgt gaactggcaa agagactgaa catgccggtt
    21661 tccatggact ttatgtccgt atccagttac ggcgcaggca ctgtgtccag cggtgtggta
    21721 aggatcatca aggacttaga tgaaccgtta gagggaaaga atgtcctgat cgtagaagat
    21781 atcattgatt ccggccgtac cctggcttac ctgatcgaag tattaaagca gagaggaccg
    21841 aaggatatcc atctttgtac ccttcttgac aagccggagc gccgtgtaaa gaagcaggta
    21901 aaagtagatt acacatgctt taccattccg gatgagtttg tagtaggtta tggactggat
    21961 tatgaccaga aataccgcaa ccttccttat atcggagtgg tagagcagga tcaggaagag
    22021 acacaggaat aattccgggt cctaaaatta aggaggcaat tcgttgaaac agcaacagac
    22081 attcagaggt tttatgttta ttatcctgat gctgatcctt cttatgcttg cgctgcgttt
    22141 tccttatgca aggcaggcac agacggtaac agcccaggaa tttgagcaga tcttgggaag
    22201 cggaaacgtg gcagaggcag tgatcagccc aaatccccag acaccaagcg gatatgtgac
    22261 agttacactg gcaaatggcc agacagtccg ccagaacgta tctgacgtaa aagatgcaga
    22321 gcagctttta aaggacaata gcattgatta ttccatggag gatgtacctc aggagaatac
    22381 ccttcttact gtggtaatgc ctttcatgct ttccatcgta gtggtggtag tgatcgttac
    22441 catgatgaac cgtggagcag caggcggcgg ttccaatgcc agaatgatga actttggcag
    22501 aagccgtgca aaactcagcc gtgacagcaa ggtgaatttc accaatgtag ctggtctgga
    22561 agaggaaaaa gaggagctgg aagaagtagt ggatttctta aaaaatcctc agaaatacac
    22621 cagcgtaggt gcacgtatcc ctaagggaat gcttttagta ggccctccag gaaccggtaa
    22681 gacccttctt gcaaaggcag tagccggaga agcgggagtt ccattttttt ccatttccgg
    22741 ttctgatttc gtagaaatgt tcgtaggtgt gggcgcttcc cgtgtccgcg acctgtttga
    22801 agaagcaaag aaaaatgcac catgtatcgt tttcattgat gagatcgatg cagtggcccg
    22861 tcgcagaggt accggaatgg gcggcggaca tgatgagaga gagcagacat taaaccagtt
    22921 actggttgag atggatggtt tcggtgtcaa tgaaggcatt atcgttatgg ctgctactaa
    22981 ccgtgtggat atccttgatc cggcgatcct tcgtccggga cgttttgacc gtaaggtggc
    23041 tgtaggccgc ccggatgtaa agggaagaga agagatctta aaggtacatt ccaaagaaaa
    23101 gcctttaagt gaagatgtag accttcgcag agtagcccag acaacagctg gatttacagg
    23161 tgcagatttg gaaaacctga tgaatgaggc agcaatcatt tccgcaagag acaaccgccg
    23221 ctttatccgc caggcagata ttgacaaggc atttgtaaag gttggcatcg gtgctgagaa
    23281 gaagagccgt gtgatctctg agaaagacaa gaagatcact gcttaccatg aggcaggcca
    23341 tgcgatcctg ttccatgtgc ttcctgatgt aggtcctgtg cataccgttt ccattattcc
    23401 taccggtgtt ggcgcagctg gttataccat gccgcttccg gaaaaggatg aaatgtttaa
    23461 tacaaagggt aagatgcttc agaacattat ggtagacctg ggcggacgta ttgcagagga
    23521 gatcatcttt aaagatgtga ccaccggtgc ttcccaggat atcaagcagg caagcaagct
    23581 tgcaagagcc atggtcaccc agtatggtat gtctgatcgt gtgggaatga tccagtatgg
    23641 cagtgatgag gacgaagtat ttatcggccg tgatctggca cacaccaagt cctacggaaa
    23701 cgagatcgca gatgtgatcg acgaagaagt aaagcggatc gtagatgaat gctacaccaa
    23761 ggcgaaaaat atcattctgg aacatgaaga tgtacttcat tcctgtgcag cccttctcat
    23821 cgaaaaagag aagatcggac aggaagaatt cgaggctttg tttaaaaatc acgaacctgt
    23881 catgcacaat gatggtgaga tgtaaaaata cagcaaaaag cagctgttaa aattgacagc
    23941 gaaaaaccgt gaaaaaaata tatctttgtg cacacttttg tggggagtta tgctattata
    24001 atagatgtaa ccccccaata cattatatag tttttgctac accccataag aaacgaaata
    24061 ccttctcctg aaaagcctgg ctaccccgcc aggcttttcg catatctgga tatgtggaaa
    24121 aaaatggaaa ataaagagaa gaaagttaat aaccggtata ccggtgtgtt gcatatttga
    24181 aaaaattgat ttaaaataat tatggattta ataataaata taaaagagga aaaacctgtg
    24241 aaaaataata ttcagataaa cagggatgcc ctgttttcag cagaaactca ggattaccgt
    24301 atgcctgcag agccggatat tcatgataaa gttgtagtga gaatgcgcac ggcaaaggat
    24361 gatgtggatc atgtttatta tatagaggga aagaaagaga cagagatgaa aaaaactgtc
    24421 tcagatactc tttttgatta ttatgagcac acaatgacag caggagagga acagatcctg
    24481 tatcatttta aaatagtaaa gggaacagaa gtctgtcagt ataaccgtct gggagtttct
    24541 gatcaggaca cggaccagtt tgattttaag atcacacctg gttttcacac tccggactgg
    24601 gcaaaaggtg ccgtgatgta ccagatcttt gtagaccgct tctgcaatgg agatccgacc
    24661 aatgatgtac agtcctgtga gtatgtctat attggcaaac cggtttacca ggtaaaagac
    24721 tggagcagaa atccttctac tatggacgta ggctgttttt acggcggcga ccttcagggc
    24781 gtgtgggaca aactggacta tatcaagagc ctgggcgtag aagttattta tttaaacccg
    24841 gtattcgtat ccccgtccaa ccataaatac gactgccagg attatgaaca tattgatcct
    24901 cattttggaa agatcgtaaa agacggcggt tccatggtgg atcagaatgc tgcggataac
    24961 acaaaatcag agcggtatgt agtgcgctcc gcagaccgtg aaaacctgga agccagtgat
    25021 gccttttttg cggaattcgt aaaagaagta cacaaccgcg gcatgaaggt gatcttagac
    25081 ggtgtgttca atcactgcgg atcctttaat aaatggctgg atgcagagca gatctatgag
    25141 cgttccggtg attatgagcc aggggcctat atttccaagg acagccctta tcacagcttt
    25201 ttccattttc atgacaacag tgataaagca tggccttaca accgcaccta tgacggctgg
    25261 tgggggcatg ataccctgcc aaagctgaat tatgaaggct ctgataaatt agttgagtat
    25321 attttaaatg tggcaaaaaa atgggtgtct ccgccttaca gcgtagatgg ctggcgtctg
    25381 gatgtagcgg cagatctggg acatacagca gaatataatc acgctttctg gaaaaaattc
    25441 agaaaggcgg taaaagaggc aaatccccag gcactgatcc ttgcagagca ttacggcgac
    25501 ccgtccggct ggcttcaggg agatgagtgg gactctatta tgaactatga tgcctttatg
    25561 gagccggtca catggttcct gacaggtatg gaaaagcaca gtgacagcta taatggcggt
    25621 ctttacggaa atggacagtc tttctttgat gccatggctt atcatatgag ccggatgcag
    25681 accaacacgg tgtttacagc catgaaccag ctgtcaaatc acgaccactc ccgcttcctt
    25741 acaaggacca accaggtagt aggccgtatc ggaagtatgg gaccggaacg tgcttctgaa
    25801 aatgtaaaga aatgtgttat gagagaggcg gtcatgattc agatgacctg gccgggtgct
    25861 cctacactgt attacggcga tgaggccggt gtgtgcggct ggaccgatcc ggacagcaga
    25921 agaacttatc catggggaag agaagacctg gaattaatgg aattccaccg ttatatggcc
    25981 ggtatccaca aacgtcttcc tgcactgcga aaaggcgctg taaagccatt atttgcggca
    26041 aaccagcaga ttgcctatgg ccgtatgtgg gacagatacc agacagtgac agttatttcc
    26101 aatttgccac aaccgtctgc gatagaaatt ccggtatggc agttaggcat cacagatgaa
    26161 gatattatgg gacgtcctat tataacaaca gaggacggct acaatgcagg aattttattc
    26221 taccatgtaa aagacggtat tttgaaagta agaatgcctg catacagcgg tgcggtcttt
    26281 gcttcccatc cggaagattt ttatccggtg cttccatctc gttttgtaga agagggagaa
    26341 gaagaggcaa gggaaaaaga gcagcttgag aaagctgcgg aagcataaaa agatataaaa
    26401 gagcatataa aagaagcaga ctccggtttt gctgggagtc tgcttctttt gcagttattg
    26461 tggcaagacg aaacgaaatt tgccacgctg tttacaaaaa aagaacagaa ccaatcaggt
    26521 tctgttccaa aagctgccgt agaccggaat cgaaccggta cgggtatcac tacccacggg
    26581 attttaagtc ccgggcgtct gccagttccg ccactacggc atattctttt ttcatacata
    26641 cctattgctg aaatcaggcg catacgtgca agcactgtcg ctcttagcag gtatgtaaat
    26701 gggcgaaggt ggattcgaac caccgaaggc agtgccagca gatttacagt ctgtcccctt
    26761 tggccactcg ggaattcgcc catggctttt tcaagcagta atgatgatac catagaaaaa
    26821 aggaaaaatc aatacttttt ttgaaaaaaa tttacggaat tctttgactg ataatgttta
    26881 tcatggacga atggccctgc atatggtata attcataaga taggcgtatt ccattcattt
    26941 ttgggtacga ttggaggaag actatggatg agaaaaggaa ggtgcggggg aacttccgca
    27001 tttatcttca gtggcccatt tttttatcgg tcctgatggt cctgctgaca gctgctgtgg
    27061 gggcagtttc attaagagca ggcattatag tttcattttt tactcttatc tatattggaa
    27121 t
//
LOCUS       DBMDUA010000013        26546 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1286_length_26546_cov_3.474154, whole genome shotgun sequence.
ACCESSION   DBMDUA010000013 DBMDUA010000000
VERSION     DBMDUA010000013.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 26546)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 26546)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26546
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1286_length_26546_cov_3.474154"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..92)
                     /locus_tag="ACZPCL_02125"
     CDS             complement(<1..92)
                     /locus_tag="ACZPCL_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HIR1161319.1"
                     /translation="MPDINILVVDDEKEIADLIEIYLVSDGYKV"
     gene            complement(289..2103)
                     /locus_tag="ACZPCL_02130"
     CDS             complement(289..2103)
                     /locus_tag="ACZPCL_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161320.1"
                     /translation="MKKQKRYKICRGLFFLVFLMEISGRLAFWGMAAEKESAETQTGA
                     AGAYYEEDAFTVLLETEYAEKKVSLKEAFHWCPEYLQGLRVKEVELSPGLYLTEEDPG
                     DFTGERANGQFRVCLEINDSGKELLTEDGCPGNWWIQEEETVDLNMILEFSKALTDSE
                     RERYIDVTLGNDQVTLKRRIWLVCKVIPVSFSGSGILPGSCYQLRSAETEKNKKAVID
                     EASSFTAFYNVRSFIPGNYDSRKIVWKKDGMEEGLPVGTSIIMTERSEERSTTDFWYY
                     KVQEKVSRIDLNKFTRMSGDEEYSYDMESTEYNSLDYQFMADFEESEEKAEEGLYQLG
                     FIASDSSEEVKVCQMELEVQVVPGKTYDLEIRESEEREETPKVTVTCKTEDPTVIITC
                     KAEDPREEIAQKEKTFALVLTGTGETELPEDARLKVGKREYRTSGKDSFILPLGDMQN
                     ENSNWKLTLLSDLFPEEEKEYHFTAQLYQTDPFTESMPLKGKAVTEPRELIFKKAEKV
                     RTALKITGVRISDSEGWRKGQSFNVQVLNMPPEGQITVTAYEGLTGWQRSDAWNVSME
                     EGKISNACASKGTFRLIFEIKNAAGEVVCLAPYYLIVK"
     gene            complement(2140..3699)
                     /locus_tag="ACZPCL_02135"
     CDS             complement(2140..3699)
                     /locus_tag="ACZPCL_02135"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR1161321.1"
                     /translation="MKKWIRRFVMTILGLVLGTGMYMICAERVLKDPFPMPFGIGFAN
                     VVSGSMEPALKKGTLLLVRKKADAEIGDIVVYQSGESIIVHRIISAEGDQVITKGDAN
                     ETEDIPIKRSQIKGVVMFKIPEIGKGIQWLQTLARYSGADHKQESAKVAAFEHGEQIL
                     QPFSRAGKTGDLQPGTDRVYALTVTNEKNGNVSETRQNYSIQVTTAGTLPLTYVLKRN
                     NTELGRFSEMVTENYLFQTADMWFEAGKKEGHAYELEVIWSEDKKDVLWADVPDFIKI
                     QVHMEQAETIASTLVVPVLARYHHSSTYIYEMAAVPFYFTSDRLTEEGNEYQLSPETE
                     ALSIELRNDQDGNRWSDTPVHYIYQVKKDGEEQEEKGGEGILYPQQGAGSSERIIIQD
                     MAPGVYDIEVQSLSPFEKTLKGTFVILEKENDIFCVKEDQQGSFCVQLIVSVKDYEGN
                     IKISWPEGLIPDSTQAVFEDVKTRPEDLYEAGELTLWMEPYTSRTFRFFKTDAETDYT
                     WDTKLNAEAAD"
     gene            complement(3696..4205)
                     /locus_tag="ACZPCL_02140"
     CDS             complement(3696..4205)
                     /locus_tag="ACZPCL_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161322.1"
                     /translation="MPGVFFWFILLFFRHRIYVTGFIKAAAGGTLKWSGKEEKIAVDG
                     RFVLEKVPFGKQKWEVLMPDGTAYQLVWKLERKKKEPVSFVFRENSPFKIRVGKQIRA
                     IELVLTLKESTFLPDTSYRAVIDKKQKVYIPEGVIPPEEDGSNRTPAGLKVDKNGKFS
                     IDEQRGMDK"
     gene            complement(4317..5330)
                     /locus_tag="ACZPCL_02145"
     CDS             complement(4317..5330)
                     /locus_tag="ACZPCL_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161323.1"
                     /translation="MKKNKRMRTASGLMIAALLTTSMISGTFAKYTTADAAQDSARAA
                     KWGVNLGISGSLYGDAYAGADSNTIVSNTDSSVTVKSSAAGTDVIAPGTRNSNSGLHI
                     TLNGTPEVSTRVYGTIKTQNIYLKKGEYGVMVEAHDVKAEDFANGGNEYYTETGGAYE
                     KAKGYTQGTGVTYYTLENVANVESNYYPVVYSGAKVTGSKTKDSLSEIGQNIANLLAD
                     SQPVTGSAGTGAEQGQTIYTVETDAAKIYAPGEDLADQLNNLSSTNITWEWEFEGDDG
                     ADTILGALIAGNSKVVVKDQDSNSYNKISVNEGTGLAAADNTILASVKTSFSIELTAD
                     QVD"
     gene            complement(5327..5917)
                     /locus_tag="ACZPCL_02150"
     CDS             complement(5327..5917)
                     /locus_tag="ACZPCL_02150"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR1161324.1"
                     /translation="MEKEWVSKMGTFVGTLLCILILPVVVINCTLLFKSHINRERVPN
                     IAGTFPMVVLTDSMYPEFQAGDLIFCHTKSASDVRPGDVIAFFDPAGNGKSIVTHRVE
                     SITTDENGSLAWVTKGDANNVKDNAVVPAKNLAGVYRRRIQGLGNAVVFMQTSKGMMV
                     CVVFPILLFVMYDAVRRNRYEKNRIYAMECERRNQV"
     gene            complement(5898..6647)
                     /locus_tag="ACZPCL_02155"
     CDS             complement(5898..6647)
                     /locus_tag="ACZPCL_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR1161325.1"
                     /translation="MWKIAVADTAEDHLTLLKDALKAYSQETHRVFSVTSYTDLTVLE
                     KDMAQGEHFDLLILDLVMNGQRSVEAVRRIREKEDGTPLIFLTANREYAVESYEMNAL
                     DYMIKPLKKERLVKRLDQLFKRKNPRFSVRTQGDYSYYGYDELVYFEAREHRVYGRRK
                     DGREFKCSQNLGRIEEQLREDPRFYRCHRGYIVNLDYVEQIREVMLLKGDLRVPYRLR
                     NKRKIKEDYQRYVTENICFLKEGKGSGKRMG"
     gene            complement(6902..9040)
                     /locus_tag="ACZPCL_02160"
     CDS             complement(6902..9040)
                     /locus_tag="ACZPCL_02160"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HIR1161326.1"
                     /translation="MAEKNDYVNRELSWIEFNYRVLSEARDKSTPLFDRLKFLSITAS
                     NLDEFYMVRVASLKDMVHAKYAKKDIAGLTAKEQLDKISEKTHQLVDLQYSTYNRSLV
                     PALKQNGLRIVMEHEDLTEDEAAFADEYFHKNVYPVLTPMAFDSSRPFPLIRNKTLNI
                     AALLKKKNEKEAELEFAMVQVPSVIPRIVELPEEKDENGETQRVVILLEEIIERNMQS
                     LFLNYDIVASHPFRIMRNADFSLDEEEAVDLLEEIQKQLKKRQWGEAIRLEIESNVDK
                     QLLKILKKELSISSEDIFQISGPLDLTFLMKMYGLDGFDHLKTPKYTPQPVPELMNED
                     NIFTNIRKGDILLHHPYQSFDPVVNFVRSAARDPEVLAIKQTLYRVSGNSPIIAALAE
                     AADNGKQVSVLVELKARFDEENNINWAKMLEKAGCHVIYGLVGLKTHSKITLVVRMEE
                     DGIRRYFHLGTGNYNDSTAKLYTDCGILTCDPQIGEDATAVFNMLSGYSEPRAWNKLS
                     VAPLWLRHRFLRMINRETEHARAGREAHIMAKMNSLCDKEIISALYDASCAGVKIELI
                     VRGICCLKAGVPGLSENISVRSIVGNFLEHSRIFYFFNDASPEVYMGSADWMPRNLDR
                     RVEILFPVEDENLKEKVIHILKLELEDNVKAHLLQPDGSYEKVDKRGKVLVNSQDQFC
                     EEAIEMAKACQESKDPAVTRIFQPIESSNE"
     gene            complement(9042..10607)
                     /locus_tag="ACZPCL_02165"
     CDS             complement(9042..10607)
                     /locus_tag="ACZPCL_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR1161327.1"
                     /translation="MAVRMFAAIDVGSFALELGIYEISDKTGIRNVEHIRHVIALGSD
                     TFSEGKISYDLVDEMCRVLEKFTETMKMYRVKDYRAYATTAMREAKNSRIILDQIRVR
                     TGLEVHIISNSEQRFISYKAVASKGGEFNQIIQKGTAIVDVGFGSAQISLFDKDSLVA
                     TQNLPLGSLRLRSQLAKIPASVDGHRLHIEEIVDNELITFRKMYLRDRQITNLIGIGD
                     NILYLMRRLGIKGGENRVDSAAMHAFYDKLSQMTIDQIEENFGVNSEYAALLLPAATV
                     YTRILDITGAEMFWIPAIGICDGIAAEYASDKKLIRFDHNFENDILAAARNMAKKYKC
                     HASHNQVLEQYAMNIFDSTRRFHGLGDRERLLLQIAVTLHACGKFISMKNSNECGYTL
                     IMSTEIIGLSHLEREIIANVVRYNIRDFDYDMMQLETEAGQDLAGVSDRTAITILIAK
                     LTAILRLANSMDKTHKSKLIDCRMAVREGQLVITTGYKGDLALESASFEQKAAFFEEI
                     FGIRPVLKQKRRV"
     gene            complement(10804..12071)
                     /locus_tag="ACZPCL_02170"
                     /pseudo
     CDS             complement(10804..12071)
                     /locus_tag="ACZPCL_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(12106..13260)
                     /gene="pheA"
                     /locus_tag="ACZPCL_02175"
     CDS             complement(12106..13260)
                     /gene="pheA"
                     /locus_tag="ACZPCL_02175"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR1161328.1"
                     /translation="MENLDLQEIRKKLDGIDNQLVELFEKRMALCSDVAEFKIQTGKK
                     VYDGERERQKLDSVMAMADSEFNKKGVYELFSQIMTISRKLQYGLLVRHGQALETGFT
                     MVDDLKKEGVRIAYQGVEGSYGHGAALQYFGQDADVYHVKSMEDAMVEVEEGRADYAV
                     LPIENSSAGAVSDNYDLLVKHNVYIVGETELAVNHALLGLPGATLEDIRQVYSHPQAL
                     MQCFQYLNSHRQWRQVSMENTAVSAKKVLEDADVTQAAVASEIAGKLYGLKVLQPSIN
                     YNKNNVTRFIILAKDPVYCKGANKISICFECAHKSGTLYNMLGNLIYNGVNMLMIESR
                     PIPGRSWEYRFFVDVEGTLSDPAVQNALKGISEEAATLRVLGNYEAQTDK"
     gene            complement(13324..14073)
                     /locus_tag="ACZPCL_02180"
     CDS             complement(13324..14073)
                     /locus_tag="ACZPCL_02180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1161329.1"
                     /translation="MIRLILVVLAVVLYLIFSIPVLSYLKNLEKKDPKAAAKKSLDLV
                     RWILGIVRDLAGITYEVRGLEHIPSDRAVLYVGNHRSYFDIVMGYTTVPAPTGFIAKK
                     EMIGIPLLSQWMVQMNCLFLDRSNIKEGLKTILTGIEKVKGGISMWIFPEGTRNKSED
                     PADLMEFHEGSLKIAEKSGCPVIPVAITGTDDVFECHIPWIRKSHVIIEYGEPIYLKG
                     LPLDKKKFPGAYTREIISEMIKKQQQERAGK"
     gene            complement(14139..14603)
                     /locus_tag="ACZPCL_02185"
     CDS             complement(14139..14603)
                     /locus_tag="ACZPCL_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1161330.1"
                     /translation="MKRQAAYLSILKDFFRCGIAGWCLEILFTSVEAWMRGDVKMMGT
                     TSVIMFPIYAAGAILPDIVKWCDRWLSGLPDFLKADREEISPVALKLRHGLIYMVLIY
                     TGEYIAGSFLMAKGLCPWDYSQWPDQIHGVIRLAFIPFWFGTGLLFESLCTH"
     gene            complement(14624..15070)
                     /locus_tag="ACZPCL_02190"
     CDS             complement(14624..15070)
                     /locus_tag="ACZPCL_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR1161331.1"
                     /translation="MLKRIKKSGGFSLIELLAVLAILSILMAVAVPATAGYIKSARRA
                     AAYTESQIAADAVQLYLYDEKEAGRLTVGKLHKLMNLNLSDPENVLADYISGGQKNSR
                     IVSVDADLKTGQLKKLIYENKFVKVSLTVDEDGTRNMEEDTMDKAD"
     gene            complement(15110..15472)
                     /locus_tag="ACZPCL_02195"
     CDS             complement(15110..15472)
                     /locus_tag="ACZPCL_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR1161332.1"
                     /translation="MKNRKWKEKRGFTLVELICVIAIMGILMAVAVPSYSCIQEKSAK
                     QVAISNARANYMQGKAQQEMVDAGVLADSETEAYNYDASEDKATWEGEIGKKSYKAEY
                     SGKTGEGYILSDDNGTDR"
     gene            complement(15511..17160)
                     /locus_tag="ACZPCL_02200"
     CDS             complement(15511..17160)
                     /locus_tag="ACZPCL_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR1161333.1"
                     /translation="MKITIEEMLVERDIISRQQLERVKEIQKSHEDKSLTDMLLLLGY
                     TTEDVIMNCFSDRDDMEIIHLEERTADPAAIEMLPESFIVNNEIVPVCFEKGDLLVAC
                     AFPVDPDILEEAATISGMTIRPVLTPADQIQKMLSGMETITEEKKKAAETGKTAEKVE
                     SAPAVRLVNTLIESAYKRNASDIHIEPGKEFLTIRFRIDGDLCMYTKMEMSYHRPVVT
                     RLKLMGEMDIAEKRLPQDGKYRYEKEEMATDLRISTLPSVYGEKVVLRLLGNDRDSSL
                     IDVRRLGMDEKQEEIFGRMLKAPFGIILVTGPTGSGKSTTLYAALSQLAEKKINCVTV
                     EDPVEKLINGVTQVQVNAKAGLTFATALRSILRQDPDVIMVGEIRDEETADIGVRAAV
                     TGHLVLSTLHTNDCVSAVYRLQNMGIPFYMIAAATTGVVAQRLVKVLCPHCRRKIKAG
                     QQTKRLLYEVSGKEAEEVWEAVGCEECLNTGYSHRRAIYEMFELNETTKNMIRDGETA
                     EKIRRYQRERGFLSLQEQAAQMVMNGEMDMEEAEKVIYSAD"
     gene            17229..17699
                     /locus_tag="ACZPCL_02205"
     CDS             17229..17699
                     /locus_tag="ACZPCL_02205"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR1161334.1"
                     /translation="MAAGIFFLFILLLIIAAWTDLKEGYIPDSVSILIFLLSFCSLFI
                     RSGPGLLLRIEGAVLCGGLLEGIRLISRGGIGMGDVKLMTACGFFLGITTGAVSLVFA
                     YILAGMFCLPLLIFKKVSLKTRLPMAPFFTVSILIFLFFEERIFTWYLGLWGIL"
     gene            complement(17682..18293)
                     /locus_tag="ACZPCL_02210"
     CDS             complement(17682..18293)
                     /locus_tag="ACZPCL_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161335.1"
                     /translation="MKTRIKTDRFHGDGREGGSLLYVIFFITLLSVFACGFMAVSRYH
                     IKMALKNRAYMEARLTTHMIHNSFCEAVSKGESDALRLLWNQYEGDLEDEEAEENENE
                     DIPETEGAGADGITKLDADEIQDTNERQDADERQDTDLDLEDREYITEGTGSYTDEKG
                     KPCEVRIRLKLEPVEEKAYVDTWTKISGFSMHLEGEVPLQDTP"
     gene            complement(18256..18756)
                     /locus_tag="ACZPCL_02215"
     CDS             complement(18256..18756)
                     /locus_tag="ACZPCL_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PulJ/GspJ family protein"
                     /protein_id="HIR1161336.1"
                     /translation="MRNRDGFTLLELVAALSLSAVIGIWVAGFFGSQMHEYKKQTQIL
                     KTEELADRVCFQITSRLDHAFLFRVDPKESSVLYYCEGSGELGEQWEQISEEDLGLGA
                     EKEYEVRLDFSDTQSQKAVSEITVLYKGEKVYSRTIRLSSVYGYLEDGEDEKGENENK
                     DGSLSW"
     gene            complement(18753..19067)
                     /locus_tag="ACZPCL_02220"
     CDS             complement(18753..19067)
                     /locus_tag="ACZPCL_02220"
                     /inference="COORDINATES: protein motif:HMM:NF019575.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR1161337.1"
                     /translation="MTSEKQAGFSLLEVIVSTAILFSVAGAFLSMTAANGVLLTREHS
                     LMQNVYELSELAEEGQGEWTGEELEIIFEGEEEDASEIFGKYIVTSEGGDSMTYYKRG
                     FQ"
     gene            complement(19057..19578)
                     /locus_tag="ACZPCL_02225"
     CDS             complement(19057..19578)
                     /locus_tag="ACZPCL_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161338.1"
                     /translation="MRLSKRERFLLAVLTLLLLWTGFYKFWIEPETDLLFANLQEINR
                     LEEQIKRGGPQGKQNLEQTAEKLEQAGNGQIYFADMSPEKMDRKLLQLAGETGIQLSG
                     VETGETKEMDTAGYVYTPLTLEFVCTDREQAAGFVEKINQEGAGMWIKSLQLEKDETA
                     MKGTMEVVYCHDQ"
     gene            complement(19565..20953)
                     /locus_tag="ACZPCL_02230"
     CDS             complement(19565..20953)
                     /locus_tag="ACZPCL_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161339.1"
                     /translation="MKHYIYLTEDKLYYIRKENRARGYSFMGSRTITGPETVTGAMES
                     LADIYNLKGRAVVLAIEASVKIHFMSLPASSKKTALQMAEKQLLVLGEEGERLLAADF
                     MPNNGQGMLKGTVYTVSEAWIRAIETGALITRFRIHSILPGPALLASFLEPSDYGMNK
                     GTVIFVHLADQSIVFYQMENGHCRHLEHSSLKPGMFAYLGEEKALMEEVAVLIRRFQT
                     RMKQERREFQIQGVLIQCECLKAPRSDAGVLAKMLGIPCMALGKNGIWKRQALAGELK
                     NLLYRNTGEFMEGESFYEKITEAGFPLGPVCASLVAFAVIVLSALGGQFYTAGKLERL
                     DRISADQQGNDVTQERRDLEGKISELAKLTAEKRELAGQITLDPAVFSCLEQALLPEM
                     DLETIEYQAEDASIHLKLSTSKPEQVSLFCEKIEENSGFSLQQSHWQQQEDKVSAALV
                     FTIGKGAKDEAQ"
     gene            complement(20965..22155)
                     /locus_tag="ACZPCL_02235"
     CDS             complement(20965..22155)
                     /locus_tag="ACZPCL_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR1161340.1"
                     /translation="MALYRYNARDRENRKYKGVKEAGSEAELVKALLEDGLYCCSLKN
                     EERTASMKHFTVPLKLLPPLCSQVSSMLAAGVPQAEILKTACKTAANREMKALLGELE
                     EKVHKGQTLFEAMESMHGCFPKLLVYMVQTGETSGTLDHILEKMSSYYEKEVEMAGKV
                     RIAMIYPCILFFASIGASAFLLTSVLPQFRVMLAEYELPAITRFMMKAGAYLQDNWLL
                     YVCFLPLLLLFMMALFAVPWLRLRRDQMILYIPVISGLMKTIYTSRFASAFSVLYGSG
                     TGILECMDITGHVMGNTWIEKKLIEAAVGLQKGESLSQALSRQRIFHPVFLSMVAAAE
                     ESGHLEAVLKQAGIYYEKAAEQSAVRMVTLLEPCMILIMAAVVGSIVLSIMLPVFSMY
                     TAML"
     gene            complement(22232..22411)
                     /locus_tag="ACZPCL_02240"
     CDS             complement(22232..22411)
                     /locus_tag="ACZPCL_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4250 domain-containing protein"
                     /protein_id="HIR1161341.1"
                     /translation="MSAIPKDPMILLSYINTQLRDNYSSLEELCQAMDLDEDQIKKTL
                     SDIGFEYEPEQNCFR"
     gene            complement(22650..25241)
                     /gene="clpB"
                     /locus_tag="ACZPCL_02245"
     CDS             complement(22650..25241)
                     /gene="clpB"
                     /locus_tag="ACZPCL_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR1161342.1"
                     /translation="MNINKFTQKSMEAVQNCEKLAYEYGNQQIDQPHLLYSLLTLDDS
                     LIMKLITKMGIQGDMFKNEAKQAIEKLTKVSGGGQLYISNDLNKVLINAEDEAKAMGD
                     EYVSVEHLFLALLKQPSKEMKDLFKTYGITREKFLQALSTVRGNQRVVSDNPEATYDT
                     LQKYGYDLVERARDQKLDPVIGRDSEIRNVVRILSRKTKNNPVLIGEPGVGKTAVVEG
                     LAQRIVRGDVPDGLKDKKLFALDMGSLVAGAKYRGEFEERLKAVLEEVKKSEGQIILF
                     IDELHTIVGAGKTEGSMDAGNMLKPMLARGELHCIGATTLNEYRQYIEKDAALERRFQ
                     PVMVAEPTVEDTISILRGLKERYEVYHGVKIADSALVSAAVLSDRYISDRFLPDKAID
                     LVDEACAMIKTELDSMPAELDELSRKIMQMEIEEAALKKETDRLSKERLEDLQKELAE
                     LHDKFAAGKAQWQNEKASVDKLSSLREEIEAVNRQIQDAQQKYDLNKAAELQYGKLPQ
                     LQKELEAEEEKVKNEDLSLVHESVTEDEIARIVSKWTGIPVAKLTESERSKTLHLDEV
                     LHKRVVGQDEAVEKVTDAIIRSKAGIKDPTKPIGSFLFLGPTGVGKTELAKALAESLF
                     DDESNMVRIDMSEYMEKHSVARLIGAPPGYVGYDEGGQLTEAVRRKPYSVVLFDEVEK
                     AHPDVFNVLLQVLDDGRITDSQGRTVDFKNTIIILTSNIGSQYLLEGIDENGNIREEA
                     RDAVMGELRAHFRPEFLNRLDEMILFKPLTKDNISRIVDLCVADVNKRLADRELKLRL
                     TDAAKVFITDHGYDPVYGARPLKRYLQKNVETLAARIILADGVGEGSVIVIDVDPQGD
                     HLTAKAE"
     gene            complement(25429..26478)
                     /locus_tag="ACZPCL_02250"
     CDS             complement(25429..26478)
                     /locus_tag="ACZPCL_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1161343.1"
                     /translation="MEVYTNFRILPLKLIRTPDEVFADSKLYLDIYAWGLPFVFYYNI
                     ATGIFSALGDSKTPFYFLAASSTSNIAVDIWFVKGFNMGVAGVAWATFLCQGISCILA
                     MVVIFRRLAKIEGKEKAPFFDKKILMQIVVIAVPSTLQQSFISLGNIVIQSIINGFGA
                     PVMAGYSAAVKLNNLVITSFTTLGNGISNYAAQNLGARKMPRIKEGFRAGVKMVWMLS
                     LPLFLLYFFGGNIVLKLFMDEPTELAMHTGIIYLKILSPFYFVVSAKLVADGILRGAG
                     LMKNFMVATFTDLILRVVLAFVFSKTALGATGIWCAWPVGWCVATTLSIMFYRRGPWK
                     NGQTAAESSVEMNVD"
BASE COUNT         6476 a         6714 c         5040 g         8316 t
ORIGIN      
        1 aatactttat atccatcact gaccagatag atctcgatca ggtccgcaat ctctttttca
       61 tcatctacaa caagaatatt aatatcgggc atttgattgt cctcctcttt ggttgttttt
      121 tccctttcat ccctttcatt ataccatata catatgccta cttccttact tttttcttga
      181 aaaattatgg caaaaaagga actgtgcagt aaatttcccc tgactgcagc agttccctgt
      241 ttttttcttt ctttttactg aatatagcgt tctattttca ggcggtactc attttacgat
      301 caaataataa ggtgccaaac agacaacttc tccggcagca ttttttattt caaatataag
      361 gcggaaagtt ccttttgatg cacatgcatt tgagatcttt ccttcttcca ttgacacatt
      421 ccaggcatcg gatctctgcc agcctgtgag gccttcatag gcagttactg ttatctgacc
      481 ttccggaggc atatttaata cctgaacatt aaaagactgt ccctttcgcc atccctcact
      541 gtctgatatc ctgacaccgg tgatctttaa ggcagtcctg actttttctg cctttttaaa
      601 aataagctct cttggttctg ttactgcttt tccctttaaa ggcatgcttt ctgtgaatgg
      661 atctgtctgg tacagctgcg ctgtaaaatg gtactctttt tcttcttccg gaaacaggtc
      721 agaaagtaaa gtaagtttcc agttggaatt ttcattttgc atatcaccaa gcggcaggat
      781 aaaactgtct tttccgctgg tcctgtattc tctttttccc acttttaacc gggcatcttc
      841 gggaagttcg gtctctcctg tgccggtcag taccaaagcg aacgtttttt ctttttgcgc
      901 aatctcttcc cggggatctt ctgctttgca ggttattata accgttggat cttctgtttt
      961 gcaggttact gtaacttttg gtgtttcctc tctctcctcg ctttctctta tttcaagatc
     1021 atatgttttt cccggcacca cctgcacttc cagttccatc tggcaaactt tcacttcttc
     1081 tgagctgtct gatgctatga aacccagttg atataaacct tcttctgctt tctcttctga
     1141 ctcttcaaaa tctgccatga attgatagtc caaagagtta tactctgtac tttccatatc
     1201 ataactgtat tcctcatcac cactcatacg ggtaaattta ttcaggtcaa tcctgctgac
     1261 tttctcctgc accttgtaat accagaaatc tgttgtgctt ctctcttctg accgctctgt
     1321 cataatgatc gaagtcccca caggcaatcc ttcttccatc ccgtcttttt tccagacgat
     1381 cttccggctg tcatagtttc cgggaataaa agaacgcaca ttgtaaaatg ctgtaaaaga
     1441 tgaagcctca tctataactg cttttttatt tttctctgtc tctgcagatc ttaactggta
     1501 acaggagcct gggagtattc cagagccgga aaaactgacc ggtatcacct tacagaccag
     1561 ccagatcctg cgtttaaggg taacctggtc gtttcccaga gtcacatcaa tgtaccgctc
     1621 tctttcactg tctgtaaggg cttttgaaaa ctccaggatc atattcagat ctaccgtttc
     1681 ctcttcctgt atccaccagt ttccgggaca gccatcttct gtaagcagtt cttttccact
     1741 gtcattaatc tctaaacaaa cccgaaactg tccgttggct ctttctcctg taaaatcccc
     1801 gggatcttct tctgttaaat aaagccccgg ggagagttct acctctttta ccctaaggcc
     1861 ctgtaagtat tccggacacc agtgaaatgc ttcttttaaa gaaacttttt tctctgcata
     1921 ctctgtttcc agaagcactg taaaagcatc ctcttcataa tatgctcctg ctgcccccgt
     1981 ctgcgtttct gctgactctt tttctgctgc cattccccaa aaagccagcc ttccactaat
     2041 ctccataaga aataccagaa aaaacaatcc tctgcagatt ttatatctct tctgcttttt
     2101 cataatccca tatccctttt taatgtgccc taagacatct taatctgctg cctctgcatt
     2161 taacttcgta tcccatgtat aatcggtctc tgcatctgtt ttaaaaaaac gaaaggttct
     2221 ggacgtataa ggttccatcc acaaagtcag ctcccctgcc tcatacagat cttccggcct
     2281 ggtctttaca tcctcaaaaa ccgcctgggt actgtccggt atcagtccct ccggccagga
     2341 aatctttata ttcccctcgt aatcttttac agaaacaatc agctgtacac aaaaactgcc
     2401 ctgttggtcc tcttttacgc agaaaatatc gttttccttt tccagtatca caaaggttcc
     2461 ctttaatgtt ttttcaaaag gagacaacga ctgtacctct atgtcataaa ctcccggagc
     2521 catatcctga attatgatac gctcagaact tcctgccccc tgctgtggat acaaaatacc
     2581 ttctccgcct ttctcttcct gttcttcccc atcttttttc acctgataaa tgtaatggac
     2641 gggcgtatca gaccatctgt ttccgtcctg atcattgcga agctctatag aaagcgcctc
     2701 tgtttccggt gacaactggt attcatttcc ttcttctgtc aaccggtcac tggtaaaata
     2761 aaaaggaact gcagccattt cgtaaatata ggtactgctg tgatgatacc gagcaagaac
     2821 gggtactaca agagttgatg ctatagtttc tgcctgttcc atatgtacct gtatttttat
     2881 aaaatccgga acatccgccc ataaaacatc tttcttatct tccgaccata ttacttccag
     2941 ttcatatgca tggccctctt tttttcctgc ttcaaaccac atatcagccg tctgaaacag
     3001 ataattttct gtgaccattt ctgaaaaacg tcccagctca gtattatttc tttttaatac
     3061 atatgtaagg ggcagcgttc cggctgttgt aacctgaatg gaatagtttt gtctggtctc
     3121 acttacattt ccattttttt catttgtcac tgtaagggcg tataccctgt ctgttcccgg
     3181 ctgcaaatca cctgttttcc ctgctctgct aaagggctgc aggatctgtt ccccatgttc
     3241 aaatgcagcc acttttgcgc tttcctgctt atgatctgca ccgctgtatc tggcaagggt
     3301 ctgcagccac tgtatccctt ttccaatttc cgggatctta aacattacca cacctttgat
     3361 ctgagaccgt ttaatgggaa tgtcctctgt ttcattggca tccccttttg taatgacctg
     3421 atccccttct gcggaaatga tcctgtgtac gattatggac tcacctgact ggtagactac
     3481 aatgtccccg atttctgcat ctgccttttt tcttactaaa agcagagttc ctttttttaa
     3541 agcaggttcc atacttcctg aaacaacatt agcaaagccg ataccaaaag gcatgggaaa
     3601 gggatctttt aatacacgtt ctgcacagat catgtacatc cctgtgccta ataccagtcc
     3661 cagaattgtc attacaaacc tgcggatcca ttttttcatt tatccatgcc tctctgttca
     3721 tcaatgctaa attttccgtt tttgtctacc ttcagtcctg ccggtgtccg gttgcttccg
     3781 tcttcttccg gcgggatcac accctctggt atgtatacct tctgcttttt atcaataacc
     3841 gcccggtatg aagtgtccgg aaggaatgta ctttctttta aagtcagcac cagttctatt
     3901 gcccggatct gttttcccac ccggatctta aacggactgt tttcccggaa aacaaatgaa
     3961 acaggttctt ttttctttcg ttccagtttc caaaccagct gataagcggt accatccggc
     4021 ataagaactt cccacttctg ttttccaaag ggaacttttt ccaaaacaaa acgtccatcc
     4081 acagcaattt tttcttcttt tccgctccat ttcagagttc cgcctgctgc cgcttttata
     4141 aatccggtaa cgtagatcct gtgtctgaaa aataaaagga tgaaccagaa gaatacacct
     4201 ggcaaaaaca ggtttctcca ttggaaagcc gggtcagatc tttcgcagac agcagctgta
     4261 taactttctt ttatccagat atacagtttc aggatccgtc tatacagaac atataatcag
     4321 tctacctggt cagcagttaa ttctatggag aagctggttt ttacggaggc aagtatggta
     4381 ttatctgcag cagccagacc agttccttca ttcacagaaa ttttattgta ggaatttgag
     4441 tcctgatcct tcaccacaac cttactgttc cctgcgatca gcgcccccag gatcgtatcc
     4501 gctccgtcat ctccttcaaa ctcccactcc caggtaatat tggtactgga gagattgttt
     4561 aattgatctg ccagatcttc tcccggtgcg tagatctttg cagcatctgt ttctactgta
     4621 tagatcgtct gtccctgctc tgcaccggtt cctgcactgc cggtaacagg ctgggagtct
     4681 gccagcagat tagcaatatt ctgtccgatc tcactgaggc tgtcttttgt ctttgagcca
     4741 gttacttttg caccgctgta aactacggga tagtaattgg actcaacatt agctacattc
     4801 tctagtgtat aatatgtgac tccggtccct tgcgtatacc ccttcgcctt ttcatacgca
     4861 ccacctgtct ccgtatagta ttcattcccc ccatttgcaa aatcctctgc ctttacatca
     4921 tgggcttcca ccatcacccc atattcacct ttcttcagat aaatattctg tgtcttgatg
     4981 gttccgtata ctctggtgct tacttccggg gttccgttta atgtaatgtg aagtccactg
     5041 ttagaattcc tggttccggg ggctatcaca tcggttccgg cagcagaact tttaaccgta
     5101 acggaactgt ccgtattgga gacaatggtg ttggagtctg ctcctgcgta agcatcccca
     5161 taaagacttc cgctgatccc cagatttacg ccccattttg ctgcccttgc gctgtcctgg
     5221 gctgcatctg ctgtggtgta ttttgcgaat gttccgctga tcatactggt ggttaaaaga
     5281 gctgcgatca taagtcctga tgctgtacgc atccttttgt ttttcttcat acctgatttc
     5341 tcctttcaca ttccatggca tatattctgt ttttttcata tcggtttctg cggactgcat
     5401 catacataac aaacagaagg atcgggaaca caacacagac catcattcct tttgacgtct
     5461 gcataaagac cactgcattt cccagtccct ggatccgcct gcggtataca ccggccagat
     5521 tttttgccgg cactacagca ttgtccttta cattattggc atcccctttt gtaacccatg
     5581 ccagacttcc attttcatct gttgtaattg actccactct gtgggtgaca atgctttttc
     5641 cgtttcctgc aggatcaaaa aatgcgatca catcccctgg ccttacatcc gaggcagatt
     5701 ttgtatggca aaaaatcagg tcccctgcct gaaattccgg atacatggaa tctgtcagta
     5761 ccaccattgg gaaagtacct gcaatattcg gcacccgctc cctgtttata tggcttttaa
     5821 acaacagtgt gcagtttata actacaaccg gcaggatcag gatacacaaa agcgtcccca
     5881 caaaagttcc catcttgcta acccattctt tttccactgc cctttccctc ctttaaaaaa
     5941 cagatatttt ctgttacata ccgctggtaa tcttccttta tttttcgttt gtttctgagc
     6001 ctgtagggaa cacggagatc tccttttaac aacattactt ctctgatctg ttctacgtaa
     6061 tccagattta cgatatagcc tctgtggcag cggtaaaaac ggggatcttc tctcagctgc
     6121 tcttcgatcc tgccaagatt ttgggaacat ttaaactccc ttccgtcttt tctcctgcca
     6181 tatacccggt gctccctggc ttcaaaatac accagttcat cataaccgta atagctgtaa
     6241 tctccctgtg tacggactga aaatctggga ttttttcttt tgaaaagctg gtctaaccgc
     6301 ttcaccaggc gctccttttt caaaggtttt atcatgtaat ccagtgcgtt catttcataa
     6361 ctttccacag cgtattccct gtttgcagtg agaaatataa gaggggttcc atcctccttt
     6421 tcccggatcc gccggactgc ttcaacgctc ctttgcccgt tcatcacaag atccaggatc
     6481 agaaggtcaa aatgttcccc ctgagccatg tctttttcca gaactgtaag atctgtataa
     6541 gaagtcacag aaaataccct gtgggtttcc tgggaatacg cttttagggc atcttttaaa
     6601 agcgttaaat ggtcctctgc cgtgtcagca actgctattt tccacatagg cacagattct
     6661 ccttttacat tttgtagctt tgctttattt tgtttaaact ggttgggatc aaaaaagact
     6721 ccggatagaa aagaaatatt taaaaaagat caaaataaaa gatactggta tcgtaccata
     6781 actatttttc tttgcaacca ctttttccta aaatgcagaa attctttcct aaacagcaga
     6841 ttgcaattaa aaaagtcaaa aaaaagaagg actgcgtaaa cagcccttct cagattaaat
     6901 tttattcatt actgctttcg atcggctgga agatcctggt cactgccggg tcttttgact
     6961 cctggcaggc ctttgccatt tcgatggctt cttcgcagaa ctgatcctgg gaatttacaa
     7021 ggacctttcc acgcttgtcc actttctcgt agcttccatc cggctggaga agatgtgctt
     7081 ttacattgtc ttccagttcc agtttcagga tatggatcac cttttctttc aggttttcat
     7141 cttctaccgg gaacaggatc tccacacggc gatccaagtt tcttggcatc cagtctgcac
     7201 tgcccatata aacttccgga cttgcgtcat taaagaaata gaaaatacgg ctgtgttcca
     7261 ggaaatttcc tacgatggag cgcacggaaa tattttcgct aagaccggga actcctgctt
     7321 tcaggcagca gataccacgg acgatcagct ctatttttac ccctgcacag gaagcgtcat
     7381 aaagggcgga aatgatctcc ttatcgcaaa gggagttcat ttttgccata atgtgagctt
     7441 ctcttcctgc tcttgcatgc tctgtctcac ggttgatcat gcgcaggaaa cgatggcgca
     7501 gccataaagg agctactgac agcttgttcc atgctcttgg ctctgaatag ccggaaagca
     7561 tattaaatac agcggttgcg tcttcaccga tctggggatc gcaggttaag ataccgcagt
     7621 ctgtatacag ttttgcagtg gagtcattgt agtttccggt tcccaggtgg aaataacggc
     7681 ggataccgtc ttcttccata cggacaacca gagtgatctt actgtgggtc tttaagccca
     7741 ccagaccata gataacatgg cagccagctt tctctaacat ctttgcccag ttgatattat
     7801 tttcctcatc aaaacgggct ttcagctcta ccagaacaga aacctgcttg ccgttgtctg
     7861 ctgcctctgc cagtgccgca atgatcggtg aatttccgct gacacggtat aaggtctgct
     7921 tgatggcaag gacttccgga tctcttgctg cactgcggac aaaattaacg accgggtcaa
     7981 agctctggta tggatggtgg agcaggatat cgcctttgcg gatattggtg aagatattgt
     8041 cctcattcat cagttccgga acaggctgcg gtgtgtattt cggagtcttt aaatggtcaa
     8101 acccatcaag accatacatt ttcattaaaa aggtcagatc cagtggtccg gatatctgga
     8161 agatatcttc ggagctgatg gaaagttcct tctttaagat ctttaaaagc tgtttatcca
     8221 cattggactc gatctcaagg cggatggctt cgccccactg acgcttcttt aactgcttct
     8281 ggatctcttc cagaaggtct actgcttctt cttcatctaa tgagaaatct gcatttcgca
     8341 tgatacggaa aggatgggat gccacaatgt cataatttaa gaataatgac tgcatgttcc
     8401 gctcaatgat ctcctctaac aggatgacca ctctctgggt ttctccgttc tcatcctttt
     8461 cttccggcag ctctacaatc cttgggataa cagatggaac ctgtaccatg gcaaattcca
     8521 gctctgcctc tttttcattc ttctttttca aaagagcggc aatgtttaat gttttattgc
     8581 ggatcagagg aaacggtctg gatgagtcaa aggccattgg tgtaaggacc ggatatacat
     8641 ttttgtggaa atattcatct gcaaaagcag cctcatcttc ggtaaggtct tcatgttcca
     8701 tgacgatgcg cagaccattc tgctttaaag caggtaccag ggagcggttg taggtggaat
     8761 actgcagatc taccagctgg tgagtctttt cactgatctt gtccagctgc tcttttgcag
     8821 taagtcctgc aatatctttt ttggcatatt tcgcatggac catgtctttt aaagaagcta
     8881 cacggaccat gtaaaactca tccagattag aagctgtaat gcttaaaaat ttcaggcggt
     8941 caaacaaagg ggtactttta tctcttgctt cgcttaacac acggtagtta aactcgatcc
     9001 agcttagttc gcggtttaca taatcatttt tctcagccat tttacactct cctcttctgc
     9061 ttcagcacag gacgtattcc aaatatttct tcaaagaaag ctgccttctg ttcaaaagag
     9121 gcactctcta aggccagatc acccttataa ccggtagtga tcaccagctg gccttctctt
     9181 actgccatac ggcagtcgat cagctttgac ttatgggtct tgtccataga gttggcaaga
     9241 cgcaggattg ctgtcagctt tgcgatcaga atggtaatgg ccgtccggtc agatactcct
     9301 gccagatcct gtcctgcttc tgtttccagc tgcatcatat cataatcgaa atcacggatg
     9361 ttataacgca ctacattggc aatgatctca cgctctaaat ggcttaaacc aatgatctct
     9421 gtagacatga tcagagtata gccacattca ttggagtttt tcatgctgat aaactttccg
     9481 caggcatgaa gggtaactgc gatctgaagc agcagacgtt ctctgtcccc taatccgtga
     9541 aatctcctgg tgctgtcaaa aatattcatg gcatactgct ccaacacctg attatggctg
     9601 gcatgacact tatatttttt cgccatgttt ctggctgccg caagaatatc attttcaaag
     9661 ttatggtcaa agcggatcag ctttttatcg ctggcatatt ccgctgcaat accatcacat
     9721 atgccgatcg ccgggatcca gaacatttcc gcacctgtta tatctaaaat ccttgtatac
     9781 acagttgctg ccggaagaag aagggctgca tattcactgt ttacaccgaa attttcctca
     9841 atctggtcaa tggtcatctg ggagagttta tcgtaaaaag catgcatggc agctgagtct
     9901 accctgtttt ccccgccttt tatacccaga cggcgcataa gatataaaat attgtcaccg
     9961 ataccgatca gattggtaat ctggcggtct cttaagtaca ttttccggaa tgtgatcagt
    10021 tcgttatcca cgatctcttc aatatgaagt ctgtggccgt ccacactggc aggtattttg
    10081 gctaactggc tgcgcagacg caggctgcca agaggcaggt tctgggtagc caccagagag
    10141 tctttatcga aaagggagat ctgtgcactg ccaaaaccta catccacgat ggctgtaccc
    10201 ttctggatga tctggttaaa ctcaccgcct ttggaggcaa cggccttgta gctgataaag
    10261 cgctgttccg agttgctgat aatgtgcact tccagccctg tgcgcacccg gatctggtcc
    10321 aggatgatcc ggctgttttt cgcttcacgc atggcagtag tggcataggc acgatagtcc
    10381 ttgacccggt acattttcat ggtttccgta aatttttcca atacccggca catttcatct
    10441 accaggtcat aacttatttt cccttcactg aaggtatcgc ttcccaaagc gatcacatga
    10501 cggatatgct caacatttct tatccctgtt ttatctgaga tctcatagat ccctaactcc
    10561 agtgcaaagg aacctacatc gatcgctgca aacatcctaa ctgccacttt tttcctcctt
    10621 acgagagaac tgtaatgttc ttttaaaaaa ttccccaaaa cggggaattt gaaaatagtt
    10681 ctgttttata catctgtata cagtattcta cctcaattta acctgcctta ccctcatatg
    10741 ggagtaaagg ctttgtaaat tttatgtaaa atcgcataac tacacccatt attagtccca
    10801 ttcttacttt ctgcctaaca gatgagtaat aaactgggaa gcagtacgtc cggacagtcc
    10861 gccgtggctg atttcccact tgtttgcctc taaaagcagt tcttcttccg gcatatccag
    10921 atggtatttt tctgccagag cctttacaat gccctggaac tggaacttgt ccggagaacc
    10981 atagtagatg gtcacaccga aacgggctgc taaagacagc ttctcctgaa cggtatcatt
    11041 gttgtgcaga tcatcatcta attctctctt gtcagagaat ttctcacgga tcagatgacg
    11101 gcggttggag gttgcataaa taagcacatt cttcggacgt tttccaagtc cgccctctaa
    11161 gatcgctttc agatatttat attccagctc atactcttca aaagaaaggt catccatata
    11221 gataataaac ttgtagttac ggtctttgat ctgctctaag acttcagaaa gtgccttaaa
    11281 ctgatgcttg tacacttcaa tcatacgcag tccctgtcat aatattcatt ggcaatggct
    11341 ttgatgctgg aagatttacc ggttcccgca tcaccgaaca gcaggacatt atttgcttct
    11401 tttccgctga taaaggcttc tgtattctcg atcagtttct ttttctgcat ttcatatcct
    11461 acaatgtctt taaagtaaat atgctctaca ttgatgatcg gatcaatgat cactttgtgt
    11521 tcttcttctt tttcgataat gcggaatgcc ttgtttaaac caaattttcc aacaccatat
    11581 tcgccgtaaa atgcagccat atggctctgg aattcatttt catcttctga ctgctctaaa
    11641 aggactgcca gatctacgat gcggtcacgg atccgtctgt taaatacacg gctctcctgt
    11701 ctggatgggc gatagtgggc aatttctttc cacatagctg ccggttccag tgtttccaga
    11761 tcataatcaa ataatgcctt aaaaatgcgg taatcatttt ttgccagcat atccagggtg
    11821 cctcccgcat ttccgcggat ctcgcaggtg gtagtgtagg cattttcatt gtctgcaagg
    11881 cagagcgcta agaagcagtg ccataaattg ccctcaaaac catattctac tgccagttcc
    11941 accagttctc ctgcacaggc aaacgggtct gctttctgtc cttcttttcc cagtcccata
    12001 aggcctgcca tatcctcaaa aagatcttca tgttttaaat ttctatagat cacaagttca
    12061 ctgatattca tgtttctgtc ctctttctgt attttggatc atatcctatt tatctgtctg
    12121 tgcctcataa tttcccagaa cccttaaagt agctgcttcc tcggaaatgc cttttaaagc
    12181 attctggact gcaggatcac ttaaggtacc ttccacatct acaaagaaac ggtattccca
    12241 gcttcttccc gggatcggtc tggattcgat catcagcata ttaacaccat tgtagatcag
    12301 gtttcctaac atattgtaaa gagtaccgct cttgtgggca cattcaaaac agatactgat
    12361 cttgttggct cccttacagt ataccggatc ttttgccaga atgataaagc gggtcacatt
    12421 atttttattg taattaatgg atggctggag cactttcaga ccatacagct ttccggcgat
    12481 ctcgctggct acagctgcct gggtcacatc tgcatcctct aataccttct ttgcggaaac
    12541 agctgtgttt tccatactta cctggcgcca ctgtctgtgg ctgtttaaat actggaaaca
    12601 ctgcatcagt gcctgaggat gggaatatac ctgacggatg tcctctaaag ttgctcccgg
    12661 aagtcctaaa agggcgtgat ttacggcaag ttctgtctct cctactatgt atacattatg
    12721 ctttaccaga agatcgtaat tgtcgctgac tgcccctgcg gaagaatttt caatagggag
    12781 taccgcatag tccgctcttc cctcttctac ttccaccata gcatcttcca tggattttac
    12841 atgatataca tctgcgtcct gtccaaaata ctgaagggcc gccccatgtc cataggaacc
    12901 ttccactccc tgataggcaa tgcgcacgcc ttcttttttc aggtcatcta ccatggtaaa
    12961 tccggtctca agggcctgtc catgacgtac taaaagacca tactgcagtt tgcggctgat
    13021 ggtcataatc tgtgaaaaca gttcataaac gcctttttta ttaaactcgc tgtctgccat
    13081 agccatcaca gagtctaatt tctgccgttc ccgctcccca tcgtagactt tctttcctgt
    13141 ctgaatttta aattcagcta catcggaaca gagcgccatt cttttttcaa acagttctac
    13201 taactggttg tcgatcccat ccagtttttt ccgtatttcc tgaagatcca gattttccat
    13261 aattttccat cctcgcttat gtcttttgct tatgtctttt cttttaccgc ttatgttgct
    13321 tctttatttt ccagctcttt cctgctgctg tttttttatc atctcactga tgatctctct
    13381 ggtataggcc ccggggaatt tctttttatc caaaggaagc ccctttaaat aaataggctc
    13441 tccgtattca atgatcacat ggcttttccg gatccatggg atatgacatt caaatacatc
    13501 atctgtacct gtgatcgcta cggggataac cgggcagccg cttttttctg cgatctttaa
    13561 acttccctca tggaattcca tcagatctgc aggatcttcg cttttatttc tggttccttc
    13621 cgggaagatc cacatggaaa tgccgccttt tactttctca attcctgtaa ggatcgtttt
    13681 tagaccttct tttatattgc tgcggtccaa aaacaggcag ttcatctgca ccatccactg
    13741 gcttaacagc ggaataccga tcatctcttt tttggcaata aaaccggtag gcgccggaac
    13801 tgtagtgtaa cccattacaa tgtcaaaata gcttctgtgg tttccaacat aaagtactgc
    13861 acggtcggaa gggatatgtt ccagtccccg gacctcataa gtgatccctg ccaggtccct
    13921 tacgatcccc agaatccacc gcaccaggtc aagacttttc tttgcagctg cctttggatc
    13981 ctttttttcc aggttcttta aataagacag caccggaatt gaaaagatca gatacagcac
    14041 cactgctaac accaccaaaa tcaaacgtat catatttttc tcctttgtca gtttaaactg
    14101 ttactattat ataaaaagac ctgcctaaat acaagtcatc aatgggtaca gagtgactca
    14161 aaaagcaggc cggtcccaaa ccagaagggg ataaatgcaa gacgaatgac tccgtggatc
    14221 tgatccggcc actgggaata atcccaggga cagagtcctt tggccatcag aaaagaaccg
    14281 gctatatatt ctccggtata gataagcacc atatagatca gtccatggcg cagttttaaa
    14341 gccacagggg atatttcttc cctgtcagct tttaaaaaat ctggaagtcc gcttaaccat
    14401 cggtcgcacc attttacaat atccggaaga atggcaccgg ccgcataaat gggaaacatg
    14461 ataacagagg tagtccccat cattttcaca tctcccctca tccacgcttc cacagatgta
    14521 aagaggatct ctaagcacca tccggcgatc ccgcagcgga aaaaatcttt taaaatagat
    14581 aaataagcag cttgtctttt catggcaata gtctgcccgt gtttcagtct gccttatcca
    14641 tggtatcttc ttccatattt cttgtgccat cttcatccac tgtaagactg acttttacaa
    14701 atttattttc atagatcagt ttctttaact gtcctgtttt cagatctgcg tccacagata
    14761 cgatcctgga gtttttctga ccaccggaaa tataatctgc cagaacattt tccgggtcac
    14821 ttaagtttaa attcattaat ttgtgaagct tgcctactgt taagcgccct gcttcttttt
    14881 catcatacag atacagctgt actgcgtctg ccgcgatctg ggactctgta taggcagccg
    14941 cccttctggc acttttgata taacctgcag tggccgggac cgctactgcc ataagaatag
    15001 aaaggatcgc aaggacagcc agcaattcga tcagggaaaa tccccctgac ttttttattc
    15061 tcttcaacat ttttcttttt cctgctttcc ccaaaaacct ggactgctgt taccggtcag
    15121 ttccgttatc atctgagaga atgtagcctt cacctgtttt tccggagtat tctgctttgt
    15181 aactcttctt gccgatctca ccttcccagg ttgccttgtc ttcactggca tcatagttat
    15241 aagcttctgt ttctgaatct gcaagcacac cggcatctac catttcctgc tgcgcttttc
    15301 cctgcatata gttggctctt gcattggaaa tagctacctg tttggcagat ttctcctgga
    15361 tacagctgta agaaggtact gccacagcca ttaaaatgcc catgatcgcg atcacgcaga
    15421 tcagttccac taaggtaaat cctctttttt ctttccactt tctgttcttc atacattgcc
    15481 tcccttgtat cttaacattc tcttggaatt ttagtctgca gaatagatca ctttctctgc
    15541 ttcttccata tccatttcac cgttcattac catctgggct gcctgttcct ggagagaaag
    15601 gaagcctctt tccctctggt agcggcggat cttttctgct gtttctccat cacggatcat
    15661 attttttgtt gtctcgttca gctcaaacat ttcatagatc gcccgcctgt ggctgtatcc
    15721 tgtattcaga cactcttcac agccaactgc ctcccagact tcctctgctt cctttcctga
    15781 aacctcatac aacagccgtt ttgtctgctg tccggctttt atcttccgcc tgcagtgagg
    15841 acagagcact tttaccagtc gttgcgccac tacccctgtg gtggcagcag cgatcatata
    15901 aaagggaatc cccatatttt gcagacggta gacagcagag acacagtcgt ttgtatgcag
    15961 cgttgacagc accagatgac ctgtaacagc ggctcttacg ccaatatctg ctgtttcttc
    16021 atctcgtatt tctcccacca tgatcacatc cggatcctgt cttaaaatag aacggagggc
    16081 cgttgcaaaa gtaagtcccg ccttggcatt tacctgaacc tgggtaacac catttatcag
    16141 cttttccacc gggtcttcaa cagttacaca gttaatcttc ttttctgcca actggcttaa
    16201 ggctgcataa agggtggtag attttccgct tcctgtaggt cctgttacaa gaatgatgcc
    16261 aaagggcgct tttaacattc tgccaaatat ttcttcctgc ttttcatcca ttcccagacg
    16321 gcgcacatct atcagggaac tgtccctgtc atttcccagc agtcgcaata ctactttttc
    16381 cccgtaaacg gaaggaaggg tggaaattcg cagatctgtt gccatctctt ctttttcata
    16441 gcggtacttt ccatcctggg gaagtctttt ttctgcaatg tccatttctc ccataagttt
    16501 cagcctggta accaccggcc ggtgatagga catttccatc tttgtataca tacacagatc
    16561 tccgtcaata cgaaaacgga tggtcagaaa ttcttttccc ggctctatgt ggatatcact
    16621 ggcatttctc ttatatgcag attcgatcag cgtattgacc aggcggactg caggagcgct
    16681 ttccactttt tctgctgttt ttccggtttc agctgctttt ttcttctctt ctgttatagt
    16741 ttccatcccc gacagcatct tctggatctg gtctgcaggt gtcagcaccg gccggatcgt
    16801 cataccggag atcgtcgccg cttcctctaa aatatccgga tctacaggaa aggcgcaggc
    16861 aactaaaaga tctccttttt caaaacatac aggtacgatc tcattgttca caataaagga
    16921 ctccggaagc atctctatgg cagccggatc tgcggtccgt tcctctaaat ggatgatctc
    16981 catgtcatcc cggtcgctga aacagttcat gatcacatct tctgttgtat atcccaaaag
    17041 aagcaacata tctgtcaggc ttttatcttc atgacttttc tggatctctt ttacccgttc
    17101 tagctgctgt ctggaaataa tatcccgttc caccagcatt tcttcaatgg tgatcttcat
    17161 ggtctggttg ctcctttctt ttccagatgt tatacttttt tatagatgat ttttcaggag
    17221 gtggcaatat ggctgcagga attttctttc tgtttattct tcttcttatc attgctgcat
    17281 ggacagacct gaaagaaggt tatatccctg actctgtttc catcctgatc tttttgctgt
    17341 ctttctgttc tcttttcatc cggtcaggtc ccggacttct attgcgtata gaaggcgctg
    17401 ttctctgcgg cgggcttttg gaagggatcc gtcttatcag ccgcggtggt attggtatgg
    17461 gtgatgtaaa actgatgact gcctgtggct tctttcttgg aatcacaacc ggagcggtct
    17521 cccttgtttt tgcctatata ctggcaggta tgttctgtct gccccttctc atttttaaga
    17581 aagtctccct gaaaacaaga cttcccatgg caccattctt tactgtgtcc attttgatct
    17641 ttctcttttt tgaagaaagg atctttacct ggtatctggg cctatggggt atcctgtaaa
    17701 ggaacttctc cttctaaatg catggaaaaa ccggatattt tcgtccatgt atccacatag
    17761 gctttttctt ccacaggttc taatttcagg cggatccgca cttcgcaggg tttgcctttt
    17821 tcatcggtat aacttccggt tccttctgtt atgtattccc ggtcttctaa atccaggtct
    17881 gtatcctgtc tttcgtctgc atcctgtctt tcatttgtat cttgtatttc atctgcatcc
    17941 agtttcgtta tcccgtctgc cccggcacct tctgtttctg gtatatcttc gttttcattt
    18001 tcttctgctt cctcatcctc cagatctcct tcatactggt tccacaaaag cctaagggca
    18061 tcactttctc cctttgaaac tgcttcgcag aaagaattat ggatcatatg ggtagtcagg
    18121 cgcgcttcca tgtatgcccg gtttttcaga gccattttta tatgatatct ggacactgcc
    18181 ataaaaccac aggcaaatac agaaagcagt gtaataaaaa agatcacata cagcagactt
    18241 ccgccttccc ggccatcacc atgaaagcga tccgtcttta ttctcgtttt cacctttctc
    18301 atcctctcca tcctccaaat atccataaac agaagaaagc cggatcgtcc gggagtatac
    18361 tttctctcct ttataaagga ctgttatctc agaaactgcc ttttgagact gtgtatcaga
    18421 aaaatccagc cggacctcat actctttctc agcacccaat cctaagtctt cttctgatat
    18481 ctgctcccat tgttctccta attcaccgga tccctcgcag taatacaaaa cagaactttc
    18541 tttggggtcc acccggaaaa ggaaggcatg atccagtctg gaagtgatct gaaaacagac
    18601 acggtctgct aattcctctg tcttaaggat ctgcgtctgc tttttgtatt catgcatctg
    18661 actgccgaaa aagccggcta cccagatccc tatgaccgca ctgagtgaaa gagcagctac
    18721 aagttccagc aatgtaaatc catctctgtt tctcattgga atcccctttt gtaataagtc
    18781 atagagtctc ctccttcact ggtcacaatg tattttccaa atatttcgct tgcgtcttcc
    18841 tcttctcctt caaagatgat ctctaattct tctccggtcc attctccctg tccttcttct
    18901 gccagttcag acagttcgta tacattttgc atcagggaat gttccctggt aagaaggact
    18961 ccgtttgcag cagtcatgga aagaaaggct cctgctacag aaaagaggat ggctgtgctt
    19021 acgatcacct ctaaaagaga aaagcctgcc tgtttttcac tggtcatgac aataaaccac
    19081 ctccatggtc cctttcatcg cagtttcatc tttttctaac tgcaacgact ttatccacat
    19141 accggcgcct tcctggttga tcttttccac aaatccggct gcctgttccc tgtctgtaca
    19201 cacaaattcc agtgtaagag gtgtatacac ataacctgct gtatccatct cttttgtttc
    19261 ccctgtttcc actccggaaa gttgtatccc agtttctcct gcaagctgca aaagctttct
    19321 gtccattttt tccggactca tatctgcaaa atagatctgt ccgtttccgg cttgttccag
    19381 tttttctgcc gtctgttcca ggttctgttt tccctgggga ccgccccgtt tgatctgttc
    19441 ttccagcctg ttgatctcct gtaagttggc aaacaacaga tctgtttccg gctcgatcca
    19501 gaatttataa aaaccggtcc acaacagtaa cagcgtcaga actgccagta aaaaacgttc
    19561 ccgcttactg agcctcatct ttggccccct ttcctattgt aaagaccagg gcagcgctta
    19621 ctttatcttc ctgttgctgc cagtgactct gctgtaaaga aaatccggaa ttttcttcta
    19681 ttttttcgca aaacagagaa acctgctccg gcttactggt gcttaatttt agatgtatgc
    19741 tggcatcctc tgcctgatat tctatggttt ccaagtccat ttccggcaaa agcgcctgct
    19801 ccagacagga aaataccgcc ggatccagtg tgatctgacc ggcaagttct cttttttctg
    19861 ctgtcagttt tgccagttca ctgatctttc cttccagatc ccttcgttcc tgtgtaacat
    19921 cattaccctg ttggtctgca ctgatccggt ccagacgttc cagctttccg gctgtataaa
    19981 actgaccgcc cagggcactc agaacgatga ctgcaaatgc taccagactg gcgcacaccg
    20041 gacctaaagg aaaacctgcc tctgttatct tttcatagaa gctttctcct tccataaatt
    20101 cacctgtatt ccggtaaagc agatttttca gttctcccgc taatgcctgc cgtttccata
    20161 tcccattttt tcccagagcc atacagggga tccccagcat ttttgcaaga actcctgcgt
    20221 ctgatctggg tgctttcaaa cactcacact ggatcaggac tccctgtatc tgaaattccc
    20281 gtctttcctg tttcatcctg gtctgaaaac gacggatcag aaccgccact tcttccatta
    20341 acgccttttc ttctcccaga taggcaaaca tccctggttt taaactggaa tgttccagat
    20401 gacggcagtg tccattttcc atctggtaaa aaacaatgga ctggtctgca agatggacaa
    20461 agatcaccgt tcccttattc attccataat cggaaggttc cagaaaagag gccaacagcg
    20521 caggtcccgg caaaatggaa tggatacgga aacgtgtgat cagggctccc gtttcaatgg
    20581 cccggatcca tgcttctgat actgtgtaca cagttccttt taacatgccc tgaccattat
    20641 ttggcataaa atcagctgca agaaggcgtt ctccttcctc tcccaggacc agcagctgtt
    20701 tttccgccat ctgcaaagcc gttttcttag aagaagccgg aagactcata aaatggatct
    20761 ttacagaggc ttctatagcc aggacgaccg ctcttccttt cagattataa atatctgcca
    20821 gtgattccat ggcaccagtt acagtttctg gtccggttat ggtgcgggaa cccataaaac
    20881 tgtagcctct ggctctattt tcttttcgga tgtaataaag tttatcttct gtaagataaa
    20941 tataatgttt catcgcatcc tcctttataa cattgctgta tacatactga ataccggcag
    21001 cataatggat aaaacaatac ttcccaccac tgctgccatg atcaggatca tacatggctc
    21061 cagcagtgtt accattctca ctgcagactg ttctgctgct ttttcataat agatcccggc
    21121 ctgttttaaa acagcttcca gatgtccgga ctcttctgcc gcagccacca tggaaagaaa
    21181 taccggatga aagattctct gacggcttaa tgcctgggaa agactctctc ctttttgcaa
    21241 gcctacggca gcctctatca gcttcttttc gatccatgta tttcccatta catgtcccgt
    21301 tatatccata cactctaaaa ttcccgttcc gcttccataa agaacagaga acgccgaagc
    21361 aaacctggat gtataaatgg tcttcataag ccctgagatc acagggatgt aaaggatcat
    21421 ctggtctctt cgcagacgca gccatggaac tgcaaaaaga gccatcataa acaaaagcaa
    21481 caaaggcagg aaacagacat acaaaagcca gttatcctga agataggccc ctgctttcat
    21541 cataaaacgg gtaatagccg gaagttcata ttctgccagc ataactctga actgtggaag
    21601 aaccgatgta agcagaaagg cggacgctcc tatggatgca aagaaaagga tacagggata
    21661 gatcatcgct atccgtacct ttcctgccat ttctacttct ttttcataat aactggacat
    21721 tttctctaaa atatgatcca gggttccact ggtctctcct gtctggacca tatacacaag
    21781 aagttttgga aaacagccat gcatggactc cattgcctca aaaagagtct gtcctttgtg
    21841 gactttttct tccagttcac caagaagcgc cttcatttcc ctgtttgccg ctgttttaca
    21901 ggctgttttt aagatctccg cctgaggaac acctgccgcc agcatggaac tgacctggct
    21961 gcacaaagga ggaagcagct ttaacggaac tgtaaaatgc ttcatggagg cagttctttc
    22021 ttcatttttc aaagaacagc agtacaggcc gtcttctaac agagctttta ccagttctgc
    22081 ttcacttcca gcttctttaa cgcctttata ctttctgttt tccctgtctc tggcattgta
    22141 tctgtaaaga gccatttttc ctcctttctg aagttaaaaa gcaaaaaccg gctttacctg
    22201 atcatgcttg ctcagataaa accggctctt tttagcgaaa acaattttgt tcaggctcat
    22261 actcaaaacc tatgtcagac agagtctttt tgatctggtc ttcatccaga tccatagcct
    22321 gacacaattc ttctaaggaa ctgtagttat ctcttaactg ggtatttata tagcttaaca
    22381 aaatcatagg atctttgggt attgcactca taggatcttt tcctttcttg ttaacataat
    22441 tattacaaaa aattataaca tctgttactt tccatttcaa ctgtaatctg tgcatttagg
    22501 tcctgaaaat ccgcctgttt tcttgcttta ttcactttat aatgcttata ttataggtaa
    22561 aataatgtgt attaaagcaa aaaaacaggt ctgctgcaag catttctgtt tgcagcagac
    22621 ccgggtcatc gatcagaata aagaaaccat tattctgcct tggcagtcag gtgatctccc
    22681 tgtggatcta catcgatcac gatcacggag ccttctccta ctccatctgc aaggatgata
    22741 cgtgcagcca gagtttccac attcttctga aggtaacgct tcagaggacg ggcaccatat
    22801 acaggatcat agccatgatc tgtgataaat accttggcag catctgtcag acgcagcttc
    22861 aactctctgt cagccagacg tttgttcaca tctgctacgc aaaggtctac gatacggctg
    22921 atattgtcct tggtcagagg cttaaacagg atcatttcat ccagacggtt taaaaactcc
    22981 ggtctgaagt gggctctcaa ctctcccata accgcatctc ttgcttcttc acggatattt
    23041 ccgttttcat caattccctc taacagatac tgggaaccaa tgttggaagt aaggatgata
    23101 atggtgttct taaagtccac ggtacggccc tgggagtcgg tgatacggcc atcatccagt
    23161 acctgtaaca gtacattaaa tacatccgga tgtgcctttt ctacctcatc aaagaggaca
    23221 acactgtatg gtttacggcg tactgcttct gtcagctgac caccttcatc atagccaaca
    23281 tagcctggag gcgctccgat cagacgagcc accgaatgtt tctccatgta ctcactcata
    23341 tcaatacgca ccatattgga ctcatcgtca aacagtgact ccgccagtgc ctttgcaagt
    23401 tctgttttac ctacaccggt aggtcctaag aacaggaaag aaccaattgg ctttgtagga
    23461 tctttgatac cggccttgga acgtataatg gcatctgtaa ccttttctac tgcctcgtcc
    23521 tgtcctacta cacgtttgtg aaggacttca tccagatgta aggtcttgct gcgctcactt
    23581 tctgtcagtt tcgcaaccgg gataccggtc cacttggata cgatacgggc gatctcatct
    23641 tcggttacac tttcatgtac caggctgagg tcttcatttt taaccttttc ttcctcagct
    23701 tccagttctt tctgtaactg aggcagttta ccgtactgca gctctgcagc cttgttcagg
    23761 tcgtatttct gctgtgcatc ctggatctgg cggttcacag cttcaatctc ctcacggaga
    23821 ctggataatt tatctacact ggctttttca ttctgccact gggctttgcc tgcagcaaat
    23881 ttatcatgaa gttctgccag ttctttctgt aagtcttcca gacgttcttt gctcagtctg
    23941 tccgtctctt tcttaagagc cgcttcctcg atctccatct gcatgatctt acgtgacagt
    24001 tcatccagct ctgccggcat actgtcaagc tctgtcttga tcatggcaca tgcctcatct
    24061 accaggtcaa tagccttatc tggaaggaaa cggtcggata tataacggtc ggaaagcact
    24121 gccgcgctta caagcgcaga gtctgcgatc tttacgccgt ggtaaacttc gtagcgctct
    24181 tttaaaccac gaagaatgga aatagtatct tcaactgtag gctctgctac cataacaggc
    24241 tggaaacgac gttccagagc agcatctttt tcaatgtact gacgatactc atttaaggta
    24301 gttgcaccga tacagtgaag ttcgcctctt gcaagcatag gctttaacat gtttccggcg
    24361 tccatagagc cttctgtctt acctgcgcct acaatggtgt gcagctcatc gatgaacagg
    24421 atgatctgtc cttcgctctt ctttacttct tccagaacag ctttcagacg ttcctcaaat
    24481 tcacctctat atttagcacc ggctaccaga gaacccatat ccagggcaaa cagcttctta
    24541 tctttcaggc cgtcaggaac atcaccacgg actattcgct gcgccagacc ttctacaacg
    24601 gctgttttac caacaccagg ttcaccgatc agcaccggat tatttttggt ctttcgtgaa
    24661 aggatacgga ctacgttacg gatctcgctg tcacggccga tgaccggatc cagcttctga
    24721 tctctggcac gttctaccag atcgtaacca tatttctgta atgtatcgta agttgcctcc
    24781 gggttgtcgc ttaccactct ctgattgcct cttacagtgg aaagtgcctg aaggaacttt
    24841 tctctggtaa taccataggt cttaaacagg tccttcattt ccttagaagg ctgttttaac
    24901 agagccagga aaagatgttc tacggaaaca tattcatctc ccatggcctt ggcttcatct
    24961 tctgcattga tcaggacttt gtttaagtca ttgctgatat acagctgtcc gccaccgctt
    25021 accttggtca gtttctctat ggcctgcttt gcctcgtttt taaacatgtc gccctgaatg
    25081 cccatctttg tgatcagctt catgatcagg ctgtcatcta acgtcagcag gctgtacagc
    25141 agatgcggct ggtcgatctg ctggtttcca tattcatatg ccagcttttc gcagttctga
    25201 actgcttcca ttgatttctg tgtaaattta ttgatattca tatgcccact cccctttccg
    25261 gaacagtgtg tttgttatct tgttttttgt tctatgcgta ctataacact tgttgttagc
    25321 actgtcaaga gtcgagtgct aattttttgt aaaaagtttt tttattaaaa aatcccccgt
    25381 ctatttcata agacgagggg taatattata agttgatctg tttttatttt aatctacatt
    25441 catttctaca ctgctttcgg ctgcagtctg cccatttttc cacggtcctc ttctgtagaa
    25501 cataatggat aatgttgttg caacacacca gcctaccggc catgcgcacc agatacctgt
    25561 tgctcccagt gctgtcttag agaatacaaa ggcaagtaca acacgaagga tcaggtctgt
    25621 aaaggttgct accataaagt tcttcatcag tccggcacct cttaagatac catctgctac
    25681 cagctttgca gaaactacaa agtagaacgg agaaaggatc tttaagtaaa tgatacctgt
    25741 atgcattgcc agttctgtag gctcgtccat aaagagcttt aagacaatgt ttccgccgaa
    25801 gaagtacagt aaaaacaggg gcagacttaa catccatacc attttaacac cggcacggaa
    25861 gccttctttg atacgtggca tttttcttgc gcccaggttc tgtgctgcgt agttggaaat
    25921 accatttcca agtgtggtga aagaagtgat gaccaggtta tttaacttga cagccgctga
    25981 gtaacctgcc ataacagggg ctccaaatcc gttgatgata ctctggatca caatgtttcc
    26041 aagagagata aaactctgct gtaaggtact tggcactgcg atcactacga tctgcattaa
    26101 gatcttttta tcgaaaaatg gtgctttttc ttttccttcg atctttgcaa gtctgcggaa
    26161 gataactacc attgcaagga tacagctgat accctgacag aggaaggttg cccatgcaac
    26221 acctgcaaca cccatgttaa atccttttac aaaccagata tctactgcga tattggaagt
    26281 agaggaagca gccaggaaat agaacggtgt cttggagtct cccagtgcag agaaaatacc
    26341 ggttgcaata ttataataga acacgaacgg caggccccat gcataaatat ccaggtacag
    26401 tttggagtct gcaaatactt catccggagt acggatcagt tttaacggga gtatcctaaa
    26461 gtttgtgtaa acctccaaac tgatgtaaga taaaattact cagtttggag gttattttta
    26521 tggcaagaag aaaagacagc ccacaa
//
LOCUS       DBMDUA010000014        26056 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1302_length_26056_cov_2.252668, whole genome shotgun sequence.
ACCESSION   DBMDUA010000014 DBMDUA010000000
VERSION     DBMDUA010000014.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 26056)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 26056)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26056
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1302_length_26056_cov_2.252668"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="ACZPCL_02255"
     CDS             <1..289
                     /locus_tag="ACZPCL_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HIR1161344.1"
                     /translation="RHEPSANDKMMYDWIIYNGYHPVIIATKLDKINRSQIQKNVKIV
                     RQGLGMEKDGIIIPFSAETKQGRDEIWRLIEDTMADVNENTAAEAEEKTEE"
     gene            complement(440..958)
                     /locus_tag="ACZPCL_02260"
     CDS             complement(440..958)
                     /locus_tag="ACZPCL_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HIR1161345.1"
                     /translation="MLTEPMTLYKLMNLYLLKQVNFPLTNAQLTSFFTEHDYTTYFTL
                     QQALNELEDAGLIHKEASHSSTRYDITKEGEETLNFFGKNISPAIINDMDEYLKENKF
                     RLREEVGTTADFYKGTNQDYIVHCEVRENKAVLIGLDLSVPDKEQAEAVCGAWRRKSQ
                     EIYSHVMHTLLS"
     gene            1060..1221
                     /locus_tag="ACZPCL_02265"
     CDS             1060..1221
                     /locus_tag="ACZPCL_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161346.1"
                     /translation="MLSWGLGAMCQWQMFSTNRSEAKMRECKAYSNPAFEKLTDKYAR
                     VHIDTGGSV"
     gene            1218..4793
                     /locus_tag="ACZPCL_02270"
     CDS             1218..4793
                     /locus_tag="ACZPCL_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5717 family protein"
                     /protein_id="HIR1161347.1"
                     /translation="MRERINRLARGIIDSSVPELVIKPERIEGVLSPGESVRGEFSVQ
                     SGNDLYIKGLVYSGNERVKLESEAFGGLRNHIIYNIDTRYLEDGDTVKGSFYLVTNGG
                     EKEIPYSLRIQTGNRTEELGSLKTPRDFAALAKKNWELALRLFEYQDFTEAPFMQDVQ
                     ARTIYEGLKGRNGRNNLLEEFLVALHVKEAVSLRTDGEKILLNAPETITEGVIGLTAS
                     GWGYVKIQVEADGDFLAVEKNAVTTREFDQGNCQIHYQILPQYLHVGRNFGSVRIRSL
                     RQEFVFPVEVIKKNVQGADGREKKTADKASVCEFSRLRLKQEFTDEGDFSVNSGLLKK
                     TENLRARYPEDIAVRLWRTEQLFKAGRKDAYTAALAEIGDPVLRCRDTRPEIYCFYQY
                     LELLNEPSSEEKKAALARYIRKLLNENRKTDGWLLNLLVKTDRTCLQNPLELYEQMRS
                     MFGYGCRSPFLYGLACRLLKMHPDLFVKMGEFEIQVFLGGLKNGWLDQNLAVKAALSL
                     SEVKHYRKILERLLVNLYRSYPEMQVLEALCGLLIRGEKKDRSAFGWYEKALKAHINL
                     TRLYEYFLYSLPDDYGHALPKEVLLYFSYDKSLDQANKSVLYKNILQYVNPSTELYQV
                     YVREMEQFAMEQLLAGRMNGCLAVIYEHMIYTDMIDPRAAGILPKILCSSRVKCDDPR
                     MKYVIVRCEELKEEEAFLLEHGEAYVPAVSKNTVLFFQDAYGGRYLNISHRKLPVMDR
                     KELLDRCYEVYPDHPILKLRACEAVLKKGVEKEEEALLLEDTLSQLKLNPVFEKKLLH
                     LVTEYYCKKAVSKEEEERCAFLIQLDKDLLEPSMRRKICEIFIDCNYLREAYNMLLTY
                     EIEDVDPEKLLKLCTRMILQQLFDQDDRLLELSWRVFEAGKADSVILDYLCEHFNGTT
                     SQMYEILIQAVGEHVETYDLEERLLAQMLFTGSCDQIDSVFDLYMKRKRTRESMVKAY
                     FTQKCIQYFLEEKEIDSQIFQYLKNAVGADPGKEKIPTIYLLALTRYYAGQAEITADE
                     KKQLQIMVNILLESEMVFPYMKDLGRFVSIPEDIMDKTMIEYRGDKNVQPWIEMRILP
                     QEDHFTREEMKRVYQGIYVKKLTLFKGELLEYQIYERGEKGAVLVKEGRTCCDPERAG
                     RENSRFACLNQMSEAVAKQDAEGLLGAMEDYVKRSAALGILFPVN"
     gene            4863..6086
                     /locus_tag="ACZPCL_02275"
     CDS             4863..6086
                     /locus_tag="ACZPCL_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HIR1161348.1"
                     /translation="MEGLVIGLDLNDDYSQISCNEKEKSWTVPTVICRRKEKETWLIG
                     EEAYAATLLGEGVIVDKLLKMVRKEGTSTIGGICYTGTDLLKIFLKKLLDCPFKEFHT
                     DQVEQLVITMHGTDAKVTDCLMYCADYLNIPRDRVHVISHTESFAYYVLSQRKELWSN
                     QVGLFELSEDRLCYYEMKVQRGMRRNMVQAEAQNQEEAFDLDILDSPSGSKLADQILC
                     SCGEKLLENKLFSTVFLTGKGFERQDWATGFMKLACNRRRVFVENVLFSKGAAIKARD
                     YMVQGTEFPYVFVCEGRLKAEVALKVMRAGKEIMLAVASYGDNWYESKSSLELILDGQ
                     KEIEFLISHLDSRKKRAVSVPLTGFPDRPEKTTRIRMNIGFTDENTMAVVIRDMGFGE
                     LFPASDAVIRQEVIL"
     gene            6083..6898
                     /locus_tag="ACZPCL_02280"
     CDS             6083..6898
                     /locus_tag="ACZPCL_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161349.1"
                     /translation="MSLLLCRREKVKHPFYIEVLGIHIYSSQELCYVIYHHPLLVMND
                     FVDASLFFFVKNELGLGFLAGRMEKLVEAGGRPEEALYLFLSECDYYTEKEIQKFKQV
                     TASYRNLSQNAYGKAVADYLFSQKQYGKALQKYEKLTEEGERKKGEEAFWSQVYQNLG
                     AACAQMFQFSRAYKAYDTAYGLKEEDQILEKIYFLTCFAPGLSVDESYEALFKPEWKE
                     EWKGKLSQAETDAKQAASVRNLRALWKQDPEGQLEKAKKLISKWKSEYRKQEA"
     gene            6901..7371
                     /locus_tag="ACZPCL_02285"
     CDS             6901..7371
                     /locus_tag="ACZPCL_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR1161350.1"
                     /translation="MAFQAAKEFLEKRGFGDRIKEFDVSSATVELAAVAVGCEPAHIA
                     KTLSFLTGEGPVLIVAAGDAKIDNHKYKSFFGVKAKMLTVEQVSELIGHEVGGVCPYG
                     IKEGVKVYLDESLRRFDVVYPACGSSNSAVKLSIPDLEKSCDFEQWVDVCKLPQ"
     gene            8020..10662
                     /locus_tag="ACZPCL_02290"
     CDS             8020..10662
                     /locus_tag="ACZPCL_02290"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR1161351.1"
                     /translation="MKELEKTYNPAEIEDRLYDKWLKGKYFHAEVNRSKKPFTIVMPP
                     PNITGQLHMGHALDNTMQDILIRYKRMQGYEALWQPGTDHAAIATEVKVINKLKEEGI
                     SKADLGREGFLKECWKWRDEYGTRIVKQLHKLGSSADWDRERFTMDEGCSDAVLEVFI
                     KLYEKGYIYKGSRIVNWCPVCKTSISDAEVEHVEQDGFFWHINYPVVGEPGRFVEIAT
                     TRPETLLGDTTVAVNPDDDRYKDIVGKMLQLPLTDRQIPVIADEYVDKEFGTGCVKIT
                     PAHDPNDFEVGKRHNLEEIVILNDDATINVPGPYFGMDRYEARKAMVADLDKMGLLVK
                     VVPHSHNVGTHDRCKTTVEPMVKQQWFVKMDEMAKPAIEALKSGQLKFVPESFGKTYL
                     HWLENIRDWCISRQLWWGHRIPAYYCQDCGDVVVAKEAPTVCPKCGKNHFKQDEDTLD
                     TWFSSALWPFSTLGWPNKTEDLDYFYPTDVLVTGYDIIFFWVIRMVFSGIEQTGKCPF
                     NTVLIHGLVRDSQGRKMSKSLGNGIDPLEVIDKYGADALRMTLITGNAPGNDMRFYWE
                     RVENSRNFANKVWNASRFIMMNIEKAADVSGVTEKDLTLADKWILSKVNALTKDVTEN
                     LDKYELGIALQKVYDFIWEEFCDWYIEMVKPRLYNEADETKAAVIWTLKNVLIQALKL
                     LHPFMPFITEEIFCNLQEEEDTIMLSNWPVYKEEWAFEAEHEATETIKEAVRAIRNVR
                     TSMNVPPSKKATVYVVSEDDAVLKIFENSRSFFASLGYAGEVILQKDKAGIGEDAVSA
                     VIHKAVIYMPFAELVDIEKEVQRLKTEEKRLEGELKRSHAMLGNEKFVSKAPQAKIDE
                     EKAKLEKYTQMMAQVKERLAQLEK"
     gene            10790..>11693
                     /locus_tag="ACZPCL_02295"
     CDS             10790..>11693
                     /locus_tag="ACZPCL_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1161352.1"
                     /translation="MKLKNALILLLTATIWGVAFVAQSVGMDYVSPFTFNGVRSIIGG
                     LVLIPVILVLDRKKKAEGKDYKAPGDRKTLILGGICCGVALCVASNFQQFGVMYTSVG
                     KVGFITACYIIIVPILSLFLGKKCSPAIAGAVVLAVAGLYLLCMSGSDHGLQKGDFLI
                     MICSFIFSIHIMIIDYFSPAVDGVKMSCIQFLVSGTISLICMTIFEEPHIAQILAAWK
                     PILYAGVMSCGMGYTLQIVGQKGVNPASASLILSLESSISVLAGWLILGQKLTGREIL
                     GCVLMFSAIVLAQVPDVILSRKTGE"
     assembly_gap    11694..11703
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11755..13059
                     /locus_tag="ACZPCL_02300"
     CDS             11755..13059
                     /locus_tag="ACZPCL_02300"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR1161353.1"
                     /translation="MEAEAYLNRLPMWANKKNSLKDIRIFLDQMGGPDRKIPAIHVAG
                     TNGKGSVCAFMTSVLKKAGLRTGTFISPHLVEIRERFLINGEPAEKNAFEEAFETVKK
                     QAEHMVEEGFCHPTFFEFLFYMAMVLFAREKVDVMVIETGLGGRLDATNVLESPKVCV
                     ITSISRDHMQYLGNTIPQIAGEKAGIIKMGVQVVFDDTDKDASAVIGAKAKEMKAVSL
                     PVSKLLYDTEDSSEGILLKEKGEKGLRLSIPFEAPYQAQNAEIAVTALRILRDQKGIF
                     TGITDAVICEGISAARWPGRMEKASENVYLDGAHNPGGIAAFIQAAGKIQKRTGKKAW
                     LLFAAVADKEYETMAKELCEGLNWAGIGVVHMNSDRGLSAGRLSDVFKTYASCQVVSY
                     EDTEEAMEQMQNRAGDDLLFCAGSLYLIGELKVQLKKGEKIQ"
     gene            13056..13202
                     /locus_tag="ACZPCL_02305"
     CDS             13056..13202
                     /locus_tag="ACZPCL_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161354.1"
                     /translation="MIDFEEEIERFKPSLDVEAVEDAIVKSDLTDMTDIMMELIKERD
                     GNKR"
     gene            13202..14506
                     /locus_tag="ACZPCL_02310"
     CDS             13202..14506
                     /locus_tag="ACZPCL_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1161355.1"
                     /translation="MDIAAKNRQIANSYYNLGLEKAKVRDLSGAAQCLKKSLHFSKYQ
                     TDARNLLGLIYYENGEVADALVQWVISLNLQPENNLADHYLDEIQRKPGQLETESQNV
                     KTFNQALWHAQNGSDDLAILQLARVVAAKPHFVKAHLLLALLYMRREDYNKAGRSLFK
                     ILQIDKSNPKALYYMSLVKQNTGRADAEKRKMIKAFSHRQMQDDDVIIPNTYKENTGL
                     STVFHIIIGLIMGLVAFYVLVLPARESSLNRVRDDELIGYMQQLNNANQQNDSLQQEN
                     DDLKAQKDEVQKKLDELTTGNTSVLAQYQSLIWVLQYYRTGDLVTAAKTFADANFDMI
                     EDENIQGIISNIRQDMAGNIYQSLVQQGLQLWNGGDKTQAMDYFQASLKIQPDNPEAM
                     FYVGRLYQENGDTENANAMFDKIVGEHGDSEYAQRAATARGY"
     gene            14722..15066
                     /locus_tag="ACZPCL_02315"
     CDS             14722..15066
                     /locus_tag="ACZPCL_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR1161356.1"
                     /translation="MKQAKITYEALGDTLLIHMPKELDHHNCRDLKRDTDLMLDENYI
                     NRIIFDFGKTEFMDSSGIGVLLNRYKQMAASRGTVTYYGAGPRVVRILEMGGLVRLIR
                     GFKDRESALGNL"
     gene            15077..15538
                     /gene="spoIIAB"
                     /locus_tag="ACZPCL_02320"
     CDS             15077..15538
                     /gene="spoIIAB"
                     /locus_tag="ACZPCL_02320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042174 - negative regulation of
                     sporulation resulting in formation of a cellular spore
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HIR1161357.1"
                     /translation="MEKVEGQKEQTRQKEHVRLEFESNSRNEEFARVVAAVFMSRLDP
                     TLEETQDVKTAVSEAVTNAIIHGYGKEGGEIFLDMTADPAEKTLTVCVTDTGAGIQDV
                     KKAMEPLYTTDTTGERSGMGFSFMEAFMDEVTVESEPGKGTAVTMKKHIGR"
     gene            15544..16263
                     /locus_tag="ACZPCL_02325"
     CDS             15544..16263
                     /locus_tag="ACZPCL_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HIR1161358.1"
                     /translation="MDETLKLIELAHQGDKAARDRLVTDNMGLIWSIVKRFAGRGYEQ
                     EDLFQIGSIGLIKAIDKFDLNYQVRFSTYAVPMISGEIRRFLRDDGMIKVSRSLKELA
                     GKVFLVKEELSDCYGRDPTVEEIAGKLGVSSEEVAASLEAGAQVESIYRPLSATEGEK
                     GVLMDKLEEKSEAQEQLLNRLTLEKVMKDLAPQEKELILRRYFYNQTQMETAKALGVS
                     QVQVSRLEKKCLKELRKRFEG"
     gene            16334..16570
                     /locus_tag="ACZPCL_02330"
     CDS             16334..16570
                     /locus_tag="ACZPCL_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161359.1"
                     /translation="MKMKSVKAVLIFLLLLAGMVTGYLLWSMREPEYEKEGMLVENEV
                     TAYYNMTETGIPAFAAAPASLLIEENGEGWSENL"
     gene            16567..17214
                     /locus_tag="ACZPCL_02335"
     CDS             16567..17214
                     /locus_tag="ACZPCL_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AA"
                     /protein_id="HIR1161360.1"
                     /translation="MSQTGSSQSQTVYIQLNQITELRRRDVFLKDVGEVTCRDKVILS
                     KCRALKVKSIPEKEARRYVMSALEVVRMLEALDPSIEVNNLGESDFIIAYKPPKASEV
                     CWQWIKTVFVCLVSFFGAAFAIMTFNNDVNVGDVLSQVYFQVMGRQPDGFGLMEAGYS
                     VGLAIGILVFFNHIAVKKLNTDPTPLEVEMRLYEENICKTLIDQAGRKEKEIDVD"
     gene            17204..17641
                     /locus_tag="ACZPCL_02340"
     CDS             17204..17641
                     /locus_tag="ACZPCL_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AB"
                     /protein_id="HIR1161361.1"
                     /translation="MWIKNIFLLFFGLCSGGIIAAGVYAFLAIIGVFVRMIGKTKTKK
                     NIMLYENMIILGGVLGNIMELYKVRLPLGEVFGQIFLLTGGVSVGIFTGCLAMSLAET
                     LKALPTFSRRIRLSVGLPWLIIAIAAGKCGGSLLYFWRRMAAG"
     gene            17687..18133
                     /locus_tag="ACZPCL_02345"
     CDS             17687..18133
                     /locus_tag="ACZPCL_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVA/SpoVAEb family sporulation membrane
                     protein"
                     /protein_id="HIR1161362.1"
                     /translation="MEIDKKNYADYIKEITPVHNLWASMGKAFITGGLICTLGQLIMN
                     RVTNTWGLEAEAAGAWTSIILVFLSALCTGLNIYQKLVKFGGAGALVPITGFANSVAA
                     PAVEFRSEGQVFGIGVKIFTIAGPVILYGVGVSWILGMGYWVLQKM"
     gene            18222..20876
                     /gene="recD2"
                     /locus_tag="ACZPCL_02350"
     CDS             18222..20876
                     /gene="recD2"
                     /locus_tag="ACZPCL_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009214376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIR1161363.1"
                     /translation="MEHLRCVVERITYQNEENGYTVLKCAVKNYSDLVTVVGTMPDTH
                     VGSVLSLDGFWKMNARYGRQFCAERFEETLPATVYGIEKYLGSGLVKGIGPVFARKIV
                     EKFGENTLDIIDETPEELIKVPGIGKVRAERIKTSWKDQKEIKNIMLFLQSNEVSTSH
                     ATKIYKTYGSESISIVKENPYRLADDIWGIGFKTADSIAEKMGIDKTKFVRLRSGIFY
                     TLNKLAENGHCYGEREQLVKKAKELLEVEEGEIEVTLDEMLRTKDVIQEEEAIYLPPY
                     YFSESGCAKRLLELLLTGRKSVIDAEKTLEKVVSQSQITYDEIQLEAVRTAISSKVMI
                     LTGGPGTGKTTTTLGIISAYKMAGYKILLAAPTGRAAKRMAEATGMEAKTIHRLLEYK
                     PPEGYQKNGDKPLEGDVLILDECSMVDIMLMYNLLKAIPEHMSLVLVGDIDQLPSVGA
                     GNVLRDMIESGRIPVVRLTRIFRQAQGSRIIMNAHRINKGEAIDMRGGRNSDFFFAEK
                     QTNEETVATLVQYCKENLPRYYHVDPIQDIQVLTPMQRGECGAVNLNQILQEAMNPTD
                     ICLRRGGTQYRLNDKVMQIRNDYDKEVFNGDIGTICKVDTEERELTVDFDGRKVVYDV
                     TELDELSLAYAVTIHKAQGSEYPIVVMPFTMSHFVMLQRNLLYTGVTRAKKILVLIGE
                     RKAIFYAIRNETTAGRNTKLAKRLQPESEESRAVAAALAKKLVQGEETVGADGTALQK
                     PNSAQKEKMIVYKNSIQPSMVSETPAAYEGNLWKRLSQSKFRSSFTLKANDRHYVIEK
                     GMDTVRSHATDFIRDRLAPAEPKNDGKQTPMRGHPVFIGQHATGTCCRSCLEKWHHIP
                     KGRELTETEQKYVVDVIMEWIKRQMQTL"
     gene            20774..21466
                     /locus_tag="ACZPCL_02355"
     CDS             20774..21466
                     /locus_tag="ACZPCL_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024700.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1161364.1"
                     /translation="MASHSKGKRTDRNRAKICGRCDHGVDQKADADLIRELYEMVTNL
                     DVLQLSDGEKCTLALFGDIARRLAIANPSLDDPLQGDGVVLIDELELHMHTSWQRKVI
                     SMLKKTFPNIQFIITTHSPQILGEVDHDFNVYALSRNGSDIQVVQTTGMFGMDSNSIL
                     EDKMETDSVSAIIKQKTEEMYSLLELKDFDGAENIADEIDELTQNRNIDTVRARVIIR
                     RGRYKNNDLSNP"
     gene            complement(21515..21658)
                     /locus_tag="ACZPCL_02360"
     CDS             complement(21515..21658)
                     /locus_tag="ACZPCL_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161365.1"
                     /translation="METLNLKPIEKAKIDCARKLFHNFSSADVVYDSVDSYQHLLDIM
                     NKL"
     gene            complement(21630..21959)
                     /locus_tag="ACZPCL_02365"
     CDS             complement(21630..21959)
                     /locus_tag="ACZPCL_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161366.1"
                     /translation="MAEMKFKFTIQPYQTEAVDSVVGVFAGQPFNDHFTYRRDVEIKR
                     DVTNWMPSDEELYMGFANAKVQLDYGQLLDNIHRIQARNNIKLSDSLKSGGLGVCSHG
                     NIKFEAY"
     gene            complement(21959..22492)
                     /locus_tag="ACZPCL_02370"
     CDS             complement(21959..22492)
                     /locus_tag="ACZPCL_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161367.1"
                     /translation="MVQLPETIQNAQYSNICEIGEERIRRAGKKIKEENPLTTTNLDI
                     GFRVFKIDSSNMEDVYYRPADYNQGQMELFADNIKPDRTPEDLLFQVMLDLGILLSSD
                     IQETEIAGKKVFSVADGYLIACFDKDVTEETVKAIAQKQPVYAVFRDSSMASDSVATN
                     FEQIFETYSPRTQRKVL"
     gene            22874..25126
                     /gene="pflB"
                     /locus_tag="ACZPCL_02375"
     CDS             22874..25126
                     /gene="pflB"
                     /locus_tag="ACZPCL_02375"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HIR1161368.1"
                     /translation="MFNYEQWDGFEGRLWKEEINTRDFIQKNYTPYDGDASFLAGPTE
                     ATDKLWGILQGLQKEERAKGGVLDMDTDIVSGITSHGPGYISEETKDLEQIVGLQTDK
                     PLKRAFMPYGGIRMAEEACKNYGYEPSPELHKIFTEYCRTHNQGVFDAYTPEMKKVRH
                     NKIITGLPDTYGRGRIVGDYRRVALYGIDRLMEEKANDFANCGDGTMTDDVIRLREEI
                     SRQYQALKDMKKMAASYGYDISQPAKTAKEAVQWLYFGYLAAVKTQNGAAMSVGRIST
                     FLDIYIKRDMDKGILTESQAQELIDHMVMKFRMVKFARIPSYNQLFSGDPVWATLEVG
                     GIGVDGRSMVTKTDYRFLHTLENMGPSPEPNMTVLYSSALPENFKKYAAKISIDTSSV
                     QYENDDVMKPEWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAMNGGRDMKSKE
                     QVGPEYTPITSEYLDYDEVIRKYEVMMDWLVGVYVNTLNLIQYMHDKYFYEAAEMALI
                     DTDVRRTFATGIAGFSHVVDSLSAIKYAKVKTVRDETGLIVDFVTEGDFPRYGNDDDR
                     ADEIAVWLLKTFLEKLKRRHTYRNSEPTTSILTITSNVVYGKATGSMPDGRRAGEPLS
                     PGANPSYGAEQNGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERVDNLVQV
                     LDGYFDQGAHHLNVNVFGKEKLIDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQMD
                     VISRTCHATM"
     gene            25301..>26056
                     /gene="pflA"
                     /locus_tag="ACZPCL_02380"
     CDS             25301..>26056
                     /gene="pflA"
                     /locus_tag="ACZPCL_02380"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HIR1161369.1"
                     /translation="METFGLVDGPGVRFVVFLQGCRMRCRYCHNPETWKLEGGTPWTA
                     DKLFSHVYRYKSYWNKKGQPNGGITVSGGEPLLQIDFVTEFFKKAKEKGVHTALDTAG
                     NPFTLEEPFISKFEELMKVTDLVILDFKEIDNEKHKKLTGCPNDNIVQMAKWLSDHGK
                     DMWLRHVLVPDLTDDEEGLVRTRDLIRELKTVKRVEVLPYHTLGLFKWQKLGIPYTLD
                     GVRTPTAEEVKNFVLILEVWEEKKGQLVKEVNGF"
BASE COUNT         7901 a         4976 c         6606 g         6563 t
ORIGIN      
        1 ccgtcatgaa ccatctgcca acgacaagat gatgtatgac tggatcatct ataacggcta
       61 tcatccggtg atcattgcta ccaaattaga caagatcaac cgcagccaga tccagaaaaa
      121 tgtaaagatc gtccgtcagg gtcttggaat ggaaaaagat gggatcatta tcccgttttc
      181 tgctgaaaca aagcagggaa gagatgagat ctggaggctg atcgaagata ccatggcaga
      241 tgtaaatgaa aatacagctg cagaggcaga agagaaaaca gaagaataat taagggaaca
      301 tatataaaaa atgtgctctg acacaaaaac acgaccggga gaaaaactct caggtcgtgt
      361 tttttgatga tttattacga ttgactgcca gtatatcgca ggtgcggcgg tcatggatga
      421 tcacagtgtt tctcaaaatt taagacagca gggtatgcat cacatgagaa taaatctcct
      481 ggcttttacg tctccaggcg ccgcatacag cttccgcctg ctctttgtca ggaacagaaa
      541 ggtccagccc gatcaggact gccttatttt cacgtacttc acagtggacg atgtagtcct
      601 ggttggtacc tttgtaaaag tcggcggtgg tacctacctc ttcacgcagg cggaatttat
      661 tttcctttaa atattcatcc atgtcattga taatggcagg agaaatattt ttgccaaaga
      721 agtttaaggt ttcttcccct tctttggtaa tatcatagcg ggtgctggag tggctggctt
      781 ctttgtggat cagcccggca tcctctaatt catttaaggc ctgctgcagt gtgaaataag
      841 tagtatagtc gtgttctgta aaaaagctgg tcagctgtgc attggtaaga ggaaaattca
      901 cctgttttaa cagatacagg ttcatcagtt tatataaagt cataggttcc gtaagcatta
      961 tttcacactc cgttaaaaga tttctgaaca ttcttatcta tcataacaag tttgcttctt
     1021 taaatcaaga cagatttcac ttttaaaaca gaaggaattg tgttatcatg ggggttaggt
     1081 gctatgtgcc agtggcaaat gttcagcacc aatcgaagcg aagcaaaaat gcgggagtgc
     1141 aaagcttaca gcaatccggc atttgagaaa ctgacagata aatatgcgag agtacatata
     1201 gatacaggtg gtagtgtatg agagaacgga tcaaccgcct ggcccgcggg atcattgaca
     1261 gcagcgtgcc agagctggtg ataaaaccag agaggatcga aggggtcctt tcaccgggag
     1321 agtccgtgcg gggagaattt tccgttcaaa gcggaaatga cctgtatatt aaagggctgg
     1381 tgtattccgg aaatgagaga gtaaaattag aaagcgaagc atttggtgga cttcgcaatc
     1441 acattatata taacatcgac acccgatact tagaggatgg agatacagtt aagggatctt
     1501 tttacctggt cacaaacgga ggcgagaaag agattcctta ttctttgcgc atacagacag
     1561 gaaacaggac agaggaactg ggaagcttaa aaaccccaag ggattttgca gctctggcaa
     1621 agaaaaactg ggaactggcc ctgcgcctgt ttgaatatca ggattttaca gaagcgcctt
     1681 ttatgcagga tgtgcaggca aggacgatct atgagggttt aaagggaaga aacggcagaa
     1741 ataatctgtt agaggaattt ttagtagctc ttcatgtaaa ggaagcggtt tccctgcgta
     1801 cagacgggga aaagatcctg ttaaatgcac cggaaaccat tacagaaggt gtgatcgggc
     1861 ttacagccag tggctggggc tatgtaaaaa tacaggtaga agcagacggg gattttcttg
     1921 ctgtggaaaa aaatgcagtg accacccgtg agtttgacca gggcaactgc cagatccatt
     1981 accagatcct tccacaatat cttcatgtcg gaagaaactt tggttctgtg cgcatccgtt
     2041 ccctgcgcca ggaatttgtt ttccctgtag aagtaataaa gaaaaatgtt cagggagcag
     2101 atggaagaga gaaaaaaaca gcagataagg catctgtctg tgaattttcc cgtctgcgcc
     2161 tgaaacagga atttacagat gagggagatt tttctgtaaa cagcgggtta ttaaagaaaa
     2221 cagaaaatct ccgtgccaga tatccggaag atatagctgt acgtttgtgg cggacagaac
     2281 agctttttaa agcaggaaga aaagatgcgt atacagcggc tcttgcagag atcggagatc
     2341 ctgttttaag atgcagggat acaagaccgg aaatctactg cttttatcag tatttagagc
     2401 tgttaaatga gccttcttct gaagagaaaa aggcagcact ggcccgctac atacgtaagc
     2461 tcttaaatga aaacaggaaa acggatggct ggcttcttaa cctgttagta aaaacagaca
     2521 ggacctgcct gcaaaatccg ctggagcttt atgaacagat gcgctccatg ttcggatatg
     2581 gctgcagaag cccgttttta tatggactgg cctgccgcct tcttaagatg catccggatc
     2641 tgtttgtaaa aatgggagaa tttgagatcc aggtcttcct tggtggatta aaaaatggct
     2701 ggctggacca gaacctggct gtaaaagctg ctttatccct ttcagaggta aagcattaca
     2761 ggaagatcct ggaaagactg ttagtaaatt tatacagatc ctatccggaa atgcaggtat
     2821 tagaggcatt atgcggtctt ctgatccggg gagaaaagaa agacagatcc gcctttggct
     2881 ggtatgagaa ggccttaaaa gcccatatca atcttacaag gctttatgaa tatttccttt
     2941 attctctgcc ggatgattat ggacatgccc ttccaaaaga agtgctattg tatttctctt
     3001 atgataaatc tttagaccag gccaataaaa gtgtgctgta taaaaacatt ttacagtatg
     3061 taaacccttc tacggaactg tatcaggtct atgtgcggga aatggagcag tttgccatgg
     3121 aacagctgct tgcaggcaga atgaacggct gtctggctgt gatctatgag cacatgatct
     3181 atacagatat gatcgatccc agggctgcag ggatcctgcc aaagatcctt tgttccagcc
     3241 gcgtaaaatg tgatgatccg agaatgaaat atgtgatcgt gcgctgtgag gaattaaaag
     3301 aggaagaggc atttttatta gaacatgggg aagcttatgt tccggctgtt tctaagaata
     3361 cagtcctgtt tttccaggat gcatatgggg gaagatatct gaatataagc caccggaaac
     3421 tgccggtcat ggaccggaaa gagctccttg accgctgtta tgaggtatat ccggatcatc
     3481 ctatcttaaa gcttcgcgcc tgtgaggctg tgttaaaaaa aggcgtggaa aaagaggagg
     3541 aagcccttct tttagaagat accttaagcc agttaaagct gaatcctgta tttgagaaaa
     3601 agcttcttca tctggtaaca gaatattact gtaaaaaggc tgtttccaag gaagaagaag
     3661 agcggtgcgc tttcctgatc cagttagata aggacctgct ggagccgtcc atgcgaagaa
     3721 agatctgtga gatctttata gactgtaatt atttaagaga agcatataat atgcttttaa
     3781 cttatgaaat agaagatgtg gatccggaga agctgttaaa gctttgtacc cgtatgatct
     3841 tacagcagct gtttgaccag gatgaccgtt tactggaact gtcctggaga gtgtttgagg
     3901 ctggaaaagc ggacagcgtg atcttagatt atctctgtga acattttaac gggaccactt
     3961 cccagatgta tgagatcctg atccaggcag taggggaaca tgtggaaacc tatgatctgg
     4021 aagaacgtct gttagctcag atgttgttta caggcagctg tgaccagatt gattctgtat
     4081 ttgaccttta catgaagcgc aagcgcacca gggaaagtat ggtaaaagca tattttaccc
     4141 agaagtgtat ccagtatttc ctggaagaaa aggagatcga cagtcagatc ttccagtacc
     4201 tgaaaaatgc agtgggcgcg gatcctggca aggaaaagat ccctacgatc tatctgctgg
     4261 ctcttacccg ttactatgca ggacaggcag agatcacggc agatgagaag aaacagctgc
     4321 agatcatggt aaatattctg ttagagtcag aaatggtatt tccatatatg aaggatctgg
     4381 gacggtttgt ttctatcccg gaagacatta tggataagac catgatcgag taccggggag
     4441 acaaaaatgt acagccctgg atcgaaatgc gtatccttcc ccaggaggac cattttacca
     4501 gggaagagat gaaacgggta tatcagggca tttatgtaaa gaaactgacc ctgtttaagg
     4561 gagaactttt agaataccag atctatgaac ggggcgaaaa gggcgctgtt cttgtaaaag
     4621 aaggccgtac ctgctgtgac ccggaaagag ccggaagaga aaacagccgc tttgcctgct
     4681 taaaccagat gtcagaggca gtggcaaagc aggatgcaga agggctttta ggggcaatgg
     4741 aagattatgt aaaacgatca gctgccctgg ggatcctatt tcctgtgaac tgacgtgttt
     4801 ttgaaatggg atccaaaaag atttcaaacg tacatatgaa ctacaacgga ggaaacaaat
     4861 agatggaagg acttgtgatc gggttagatc tgaatgacga ttattcccag atcagctgca
     4921 atgaaaaaga aaagtcctgg acagttccta cagtgatctg ccgcagaaaa gagaaagaaa
     4981 cctggctcat aggagaagaa gcctatgcag ccactctttt aggcgaaggc gtgatcgtag
     5041 acaagctgct taaaatggtc cgcaaggagg gaacttctac tataggaggg atctgctata
     5101 caggtacgga tctattaaag atatttttga aaaagctgtt agactgtcct tttaaggaat
     5161 tccatacaga ccaggtggag cagctggtga tcaccatgca cggaacagac gcaaaagtaa
     5221 cagactgcct gatgtactgt gcagattatc tgaacattcc cagggaccgt gtccatgtga
     5281 tcagtcatac agaaagtttt gcttactatg tgctcagtca gcgcaaagag ctgtggagta
     5341 accaggtagg tttatttgag ttatcagaag accggctctg ctattatgaa atgaaggtac
     5401 aaaggggcat gcgccgcaac atggtccagg cagaagcaca aaaccaggaa gaagcctttg
     5461 acttggatat ccttgactcc ccttccggaa gcaagctggc agatcagatc ctttgttcct
     5521 gtggggaaaa acttctggaa aataagttgt tttcaaccgt attccttaca ggaaaaggct
     5581 ttgaacgtca ggactgggca accggtttta tgaaacttgc ctgcaaccgg agaagggtgt
     5641 ttgtggaaaa tgtgctgttt tccaagggag cagcgatcaa ggcaagggac tatatggttc
     5701 agggaacgga atttccttat gtgtttgtct gtgaaggtag attaaaggca gaagtagccc
     5761 tgaaagtcat gcgtgcagga aaagagatca tgctggcagt tgcctcctat ggggataact
     5821 ggtatgagtc aaaaagttct ctggaactga tcttagatgg acaaaaagag atcgaatttc
     5881 ttatttccca tctggactcc cggaaaaaga gagccgtttc cgtgcccctt acaggttttc
     5941 ctgaccggcc ggaaaagact accaggatcc ggatgaatat tggatttaca gatgaaaata
     6001 ccatggcggt ggtgatccgg gatatgggct ttggagagct gttcccggca tctgatgcag
     6061 tgatcagaca agaggtaatc ttatgagttt attattgtgc agaagggaaa aagtaaaaca
     6121 tcccttttat attgaagttc tggggatcca tatatattct tcccaggaac tttgctatgt
     6181 gatctaccat catccccttc ttgtgatgaa tgattttgta gatgcctctt tgtttttctt
     6241 tgtgaaaaat gagctgggac ttggctttct tgcaggacgc atggaaaagc tggtggaagc
     6301 cggcggccgc ccggaagagg ctttgtacct gttcctttct gaatgtgatt actatacaga
     6361 aaaagagata cagaaattta agcaggtaac ggcttcttac agaaaccttt ctcaaaatgc
     6421 ttatggaaag gcagtggcag attatctttt ttcccagaaa cagtatggaa aagcactgca
     6481 gaagtatgaa aaactgaccg aggaagggga acgcaaaaaa ggagaagagg cattctggtc
     6541 ccaggtctat cagaacttag gggctgcctg tgcccagatg ttccagtttt ccagggcata
     6601 taaggcatat gatacagcct atggattaaa ggaagaggat cagatcctgg aaaagatcta
     6661 ttttcttacc tgttttgctc cgggactttc cgttgatgaa agctatgagg ctctttttaa
     6721 gccagaatgg aaggaagaat ggaaagggaa actgtcccag gcagaaacag atgccaaaca
     6781 ggcagcaagt gtgagaaatt taagagccct gtggaaacag gatcctgaag gacagttaga
     6841 aaaggcaaaa aaactgatca gcaaatggaa aagtgagtac aggaaacagg aggcataatc
     6901 atggcatttc aggcagcaaa ggaatttctg gaaaaaagag gatttggaga caggatcaaa
     6961 gagtttgatg tttccagtgc aacggtagag ctggcagcag tggcagtagg ctgtgaaccg
     7021 gcccacattg caaaaacatt atctttttta acaggagaag gaccggtcct catcgtggca
     7081 gcaggagatg cgaagatcga caatcacaaa tataaatcct ttttcggtgt aaaagcaaaa
     7141 atgctgaccg tagagcaggt gtctgaactg atcggccatg aggttggggg tgtatgccct
     7201 tacggtatca aagagggcgt aaaggtgtat ttagatgagt ctttaagacg ctttgatgta
     7261 gtttatcctg catgtggaag cagcaatagt gctgtaaaac tgtccatccc ggatctggaa
     7321 aagtcctgtg actttgaaca gtgggtggat gtgtgcaagc tgcctcagta aagatacatc
     7381 tataataact ttgctcttga caaatccgta aaaaatattt atgcttgaaa acagcagtat
     7441 aaaaaatggt gatgaacaag aggagtacat ttttaagacc ctcagagaaa gcggctcact
     7501 ggctgaaagg ccgcttggga atacttgaaa atggaaggta gcttgtgagc cggacaggtg
     7561 agaaagatac attagcctgt tccgttggcc cgcgttaagg gagcgagtac gaaaggcttt
     7621 aaaatatata taagatatgt ttcaggtttt cgtattccat gagatgaccg gatatggatg
     7681 atatatgtgt tgtacatatc ggttggatca aaggtggtac cgcgatacag tcgccctttg
     7741 cttcccctat gggatgcaga gggctttttt gctgtttcaa atggatacaa cctctgccag
     7801 gaggtttact gcaaagagaa aagctgtact tattaatgtt ctctcggaat ctttttgtat
     7861 ttgattttgt gagaagattt tttgtcagtt gtgcccaaat ttctgaggcg tacgcggtgc
     7921 gtacgttgat gaaattcggg cacagctggc ggaaaatcgg ccacaaaggc aggtgcagga
     7981 aagactccga gaggacatat tataaaaagg agaattcaga tgaaggaact ggaaaagacc
     8041 tacaatccgg cggaaattga agaccgcctc tatgacaaat ggctgaaggg aaaatatttc
     8101 cacgcagaag taaacagaag taagaaacca tttaccattg ttatgccgcc gccaaatatt
     8161 acaggccagc tgcatatggg acatgcatta gataacacta tgcaggatat cctgatccgc
     8221 tataagagaa tgcagggata cgaagcatta tggcagccag gcactgacca tgcagccatt
     8281 gcaacggaag ttaaggttat taacaaactg aaagaagaag ggatcagcaa ggctgacctg
     8341 ggaagagaag gcttcttaaa ggaatgctgg aagtggagag atgaatacgg cacccgtatt
     8401 gtaaagcagc ttcacaaatt aggctcttcc gctgactggg acagagagcg tttcactatg
     8461 gacgaaggct gttctgacgc agttttggaa gtttttatca aattatatga aaaaggatat
     8521 atttataaag gttcccgtat tgtaaactgg tgccctgtat gcaagacttc tatttctgat
     8581 gcagaagtag agcatgtaga gcaggacggt ttcttctggc acatcaacta cccggtagta
     8641 ggagagccag gacgcttcgt agagatcgca acaaccagac ctgagactct cttaggcgat
     8701 actacagttg ctgtaaaccc ggatgatgac cgttacaagg atattgttgg aaagatgctg
     8761 cagctgccat taaccgaccg tcagatcccg gttattgcag atgagtatgt agataaggag
     8821 tttggtaccg gctgcgttaa gatcacacct gctcatgacc ccaatgactt tgaggttgga
     8881 aagcgccaca acttagagga gatcgttatc ttaaatgatg acgctaccat caacgtacct
     8941 ggaccatact ttggcatgga tcgttatgag gcaagaaagg ccatggtagc agacttagac
     9001 aagatgggcc tgttagtaaa ggttgttcct cattcccaca atgtaggtac ccatgaccgc
     9061 tgcaagacca cagtagagcc tatggtaaaa cagcagtggt ttgtaaaaat ggatgaaatg
     9121 gctaaacctg ccattgaagc attaaagagc ggccagttaa aattcgttcc ggaaagtttt
     9181 ggaaagactt atcttcactg gttagagaac atccgtgact ggtgtatttc cagacagtta
     9241 tggtggggcc acagaattcc tgcttattac tgccaggact gcggtgatgt agttgttgca
     9301 aaagaagctc caactgtatg cccgaagtgc ggtaagaacc atttcaagca ggatgaagat
     9361 actctggata cctggttctc ctctgcatta tggccattct ccactcttgg ctggccaaat
     9421 aagacagaag atctggatta cttctaccca actgatgtac tggtaaccgg atacgatatt
     9481 atcttcttct gggtaatccg tatggtattc tccggtattg aacagaccgg caaatgccca
     9541 tttaacacag tactgatcca tggtctggta agagactccc agggacgtaa gatgagtaaa
     9601 tcccttggaa atggtatcga tcctctggaa gttattgaca agtacggcgc agacgctctg
     9661 cgtatgaccc tgatcacagg aaatgcacct ggaaacgata tgcgtttcta ctgggagcgt
     9721 gtagaaaaca gccgtaactt tgcaaataag gtatggaacg cttcccgttt cattatgatg
     9781 aatattgaaa aggcagcaga tgtttccggt gttactgaaa aagacctgac actggcagat
     9841 aagtggatcc tttccaaggt aaatgccctg actaaggatg taactgaaaa tctggacaag
     9901 tatgaattag gtattgctct tcagaaggta tatgacttta tctgggaaga gttctgcgac
     9961 tggtacattg agatggtgaa gccaagactg tacaatgaag cagatgagac caaggctgca
    10021 gttatctgga ccttaaagaa cgtactgatc caggcattaa agctgctgca tccatttatg
    10081 ccatttatca ctgaggagat cttctgcaac ttacaggaag aggaagatac cattatgctt
    10141 tctaactggc ctgtatacaa ggaagagtgg gcatttgagg cagagcatga ggcaactgag
    10201 accatcaaag aggcagtaag agcaatccga aatgtacgta cttccatgaa cgttccgcca
    10261 agcaagaagg caactgttta tgtagtatct gaggatgatg ctgtattaaa gatctttgag
    10321 aacagccgca gcttcttcgc atccttagga tatgccggtg aagtcatcct tcagaaggat
    10381 aaggcaggta ttggcgagga cgcagtttct gctgtgatcc acaaggcagt gatctacatg
    10441 ccatttgcag agttagtaga tattgaaaaa gaagttcagc gcttaaagac agaagaaaaa
    10501 cgtctggaag gcgaattaaa gcgttctcat gctatgcttg gaaatgagaa gttcgtaagc
    10561 aaggctcctc aggccaagat cgacgaagag aaggcaaaac tggagaaata cacccagatg
    10621 atggcgcagg taaaagagcg tctggcacag ctggagaaat aatcaaatgt gcgaaagcac
    10681 gtaaaataaa aacggcccgg atggcagaac ctacatgccg tctggaccgt tttgccaaac
    10741 agggcgaaca aaaggctgaa aagcttgtat gaaaaaggaa aaacaaacga tgaaactgaa
    10801 aaatgctctg atattacttt taactgctac gatctggggt gttgcctttg ttgcccagag
    10861 cgtaggcatg gattatgtga gcccttttac ttttaatggt gtgagaagca tcatcggcgg
    10921 tcttgtgctg atcccggtga tccttgtttt agatcgcaaa aagaaggcag aaggaaagga
    10981 ttataaggca cctggtgaca gaaaaacact gatcttaggc gggatctgct gtggcgttgc
    11041 tctttgtgtg gcatctaatt tccagcagtt tggtgttatg tatacaagcg tgggaaaagt
    11101 tggctttatc acagcctgct atattatcat tgtgccgatc ctcagcctgt ttcttggcaa
    11161 aaagtgcagt ccggccatcg ccggggcagt agtccttgca gttgcaggtc tgtatttact
    11221 ttgtatgtca ggaagtgatc atggacttca gaaaggtgat ttcctgatca tgatctgctc
    11281 ttttatcttc tccatacata ttatgatcat cgactatttc tcaccggcag tagacggagt
    11341 gaaaatgtcc tgcatccagt ttctggtaag cggaactatt tccctgatct gtatgacgat
    11401 ctttgaagag cctcatatcg cccagatcct tgctgcatgg aaaccgatcc tttatgctgg
    11461 tgttatgtcc tgtggtatgg gatatacctt acagatcgta ggacaaaaag gcgtcaatcc
    11521 agccagcgct tccctgatct taagtctgga gtccagtatt tccgtacttg caggctggct
    11581 gatcttagga cagaagctga caggaagaga gatccttggc tgcgttctta tgttctcagc
    11641 gattgtgctg gcacaggttc cggatgttat tttaagtcga aaaaccgggg aaannnnnnn
    11701 nnnagagttg ataaccggaa aaaatttgta taatataaag aggaaaggaa acagatggaa
    11761 gcagaagcat atttgaaccg gcttcccatg tgggccaaca agaaaaattc cttaaaggat
    11821 atccgtatat ttttagatca gatgggcgga ccggaccgta agatccctgc tatccacgta
    11881 gcaggtacca acggaaaggg ctctgtctgc gcgtttatga cctctgtatt aaaaaaggcc
    11941 ggtctgcgta caggtacttt tatttcccct catctggtgg agatccggga acgttttctt
    12001 ataaacgggg aaccggcaga gaaaaatgcc tttgaagagg cctttgaaac agtaaaaaaa
    12061 caggcggaac atatggtaga agaaggcttt tgccatccta ctttttttga gttccttttt
    12121 tatatggcaa tggtactgtt tgcccgtgaa aaagtagatg taatggtgat cgaaacaggt
    12181 cttggaggac gtctggacgc gaccaatgtg ttagagtctc ccaaagtttg cgtgatcacc
    12241 tccatcagca gggatcatat gcagtatctt ggaaacacga tcccacagat cgcaggggaa
    12301 aaagcgggga tcataaaaat gggcgttcag gtagtatttg atgatacaga taaagacgcc
    12361 agcgctgtga tcggtgcaaa agcaaaggaa atgaaggcag tttccctgcc tgtgtcaaag
    12421 ctgctttatg atacagaaga cagctcagag ggtattttac ttaaagaaaa gggagaaaaa
    12481 gggctcaggc tttctattcc ttttgaggca ccttaccagg cacagaatgc agagatcgcc
    12541 gtaacggctc ttcgcatttt acgggatcag aaaggtattt ttacaggtat aacagatgca
    12601 gtgatctgtg aagggatcag tgcagccaga tggccgggaa gaatggaaaa agcttcagaa
    12661 aatgtttatc tggacggcgc ccacaatcct gggggaattg ctgcttttat ccaggcagca
    12721 ggaaagatcc agaaaagaac cggtaaaaaa gcctggcttt tatttgctgc tgtggcagat
    12781 aaagaatatg aaaccatggc aaaagaactt tgtgaaggtc ttaactgggc aggaatcggt
    12841 gtggtgcata tgaacagtga cagagggctg tcagcagggc ggctttctga tgtttttaaa
    12901 acatacgcct cctgccaggt cgtttcttat gaggatacag aagaagcaat ggaacagatg
    12961 caaaacagag caggagatga tctgcttttc tgtgcaggtt ccctgtacct gatcggagaa
    13021 ttaaaggtac agcttaagaa aggagaaaag atacagtgat cgattttgaa gaagaaattg
    13081 agcgtttcaa accaagcctt gatgtagagg cagtagaaga tgccattgta aaaagcgacc
    13141 ttacggatat gacagacatt atgatggaac tgatcaagga aagagacgga aataaacgat
    13201 gatggatatt gcagcaaaga acagacagat agccaacagc tattacaatc tgggtctgga
    13261 aaaggcaaag gtgcgggacc tttccggagc agcacagtgc ctgaaaaaaa gcctgcattt
    13321 ttctaaatat cagacagatg ccagaaatct tttgggtctg atctattatg aaaatgggga
    13381 agtggcagat gcattagtcc agtgggtcat cagcttaaac ctgcagccgg aaaataatct
    13441 ggcagatcat tatttagatg agatccagag aaaaccggga cagttagaaa cagaaagcca
    13501 gaatgtgaaa acatttaacc aggcgctgtg gcatgcacaa aacggcagtg atgacctggc
    13561 gatcctgcag ctggcccgtg tggtggcagc caagcctcat tttgtaaaag ctcatcttct
    13621 tctggctctt ttgtatatgc gcagggaaga ttacaataag gcaggacgtt ccctttttaa
    13681 gatcctgcag atcgacaaaa gcaatcccaa agccctgtat tacatgtctt tggtaaagca
    13741 gaatacaggc cgggcagatg cagaaaagcg gaagatgatc aaggcatttt cccacagaca
    13801 gatgcaggat gatgatgtta tcatcccaaa tacctacaag gaaaataccg gtctttccac
    13861 tgtgtttcat attatcattg gtctgatcat gggactggtt gccttttatg tactggtcct
    13921 tcctgcaagg gagagcagtt taaaccgtgt cagggatgat gaactgattg gctatatgca
    13981 gcagcttaat aatgcaaacc agcaaaatga cagcttgcag caggaaaatg atgatctgaa
    14041 agcccagaaa gacgaggtac agaaaaaact ggacgagctt acaacaggaa ataccagtgt
    14101 cctggcccag taccagagcc tgatctgggt gctccagtat taccgcaccg gtgatctggt
    14161 aaccgcagca aagacctttg cagacgcaaa ctttgatatg atcgaagatg aaaatatcca
    14221 ggggatcata agcaacatcc gtcaggatat ggcaggcaac atctatcaga gtctggtaca
    14281 gcagggactg cagctgtgga atggcggtga caagacacag gcaatggatt acttccaggc
    14341 cagcttaaag atccagccgg ataacccgga agccatgttt tatgtaggcc gtctgtatca
    14401 ggaaaatgga gatactgaaa atgccaatgc catgtttgat aagatcgttg gagaacatgg
    14461 agactctgag tacgcccaga gggcagcaac ggcaagaggg tattaaaatg tgtaaagttc
    14521 tccggttggg aaaaccgggg tcagtaattg aatattgaaa ttatacagca gagaaaagaa
    14581 taacaacctt ttctctgttt ttttggcttt acatacggat aacgcgctga ctgcgcgtat
    14641 gccagactta tatgtccggg agtttcgtct gccggtccgg cggacatccc ataataatat
    14701 tataaggagg aactgggaac catgaaacag gcaaaaatta catacgaagc attgggagat
    14761 acgttactga tccatatgcc aaaagagttg gatcaccata actgcaggga cttaaaaagg
    14821 gatacggacc tgatgttaga tgaaaactat atcaaccgca ttatcttcga ttttggaaaa
    14881 acagaattta tggactcttc cgggattggg gtcttattaa accgttataa gcagatggca
    14941 gccagcagag ggactgttac ttattacgga gcaggcccga gagtggtccg tatccttgaa
    15001 atgggcgggc tggtgcgtct gatccggggg tttaaggacc gtgaaagtgc tctgggaaat
    15061 ttataaaagc aggaggatgg aaaaggtgga aggacagaag gaacagacca gacagaaaga
    15121 acatgtaaga ctggaatttg aaagcaattc ccgtaatgaa gagtttgcca gggtggtggc
    15181 agctgtattt atgagccgct tagatcccac attggaggag acccaggatg taaaaacagc
    15241 agtgtcagag gcagtcacca atgccattat ccatggttat ggaaaagaag gtggagagat
    15301 atttttagat atgacggctg atccagctga aaaaacactg accgtctgtg tgacagatac
    15361 aggagccggt atccaggatg taaaaaaggc catggaaccc ttatatacaa cggataccac
    15421 cggggaacgt tccggcatgg gattttcctt tatggaagcg tttatggatg aagtgaccgt
    15481 ggagtcggaa ccgggaaaag ggacagcagt gaccatgaaa aaacatattg gaaggtaagc
    15541 tttatggatg agaccctgaa attgatcgaa ttagctcacc agggagataa agcagccagg
    15601 gacaggctgg taacagacaa tatgggactg atatggagca ttgtaaaacg ctttgcaggt
    15661 cgtggatatg aacaggaaga tctgtttcag atcggaagta tcggtctgat caaggcaatc
    15721 gataaatttg acctgaatta ccaggtgagg ttttctacct atgcagtacc aatgatatca
    15781 ggtgaaatcc ggagattttt gcgggatgac ggcatgatta aggtaagccg ttctttaaaa
    15841 gaactggcag gaaaagtatt tcttgtaaaa gaagaattat cggactgtta tggacgggat
    15901 ccaaccgtag aagagattgc agggaagctt ggggtaagca gtgaagaagt agccgcttcc
    15961 ctggaggcag gcgcccaggt ggagtctatt tacaggcctt taagcgccac agaaggagaa
    16021 aaaggtgttt taatggacaa gttagaagaa aaaagtgagg cccaggaaca gcttttaaac
    16081 aggcttaccc tggaaaaagt catgaaagat ctggcacccc aggaaaaaga actgatcctg
    16141 cgccggtatt tttataatca gacccagatg gaaactgcaa aagcgttagg ggtatcccag
    16201 gtccaggttt cccgcctgga aaaaaagtgt ttaaaagaac tgcgaaagcg ttttgaagga
    16261 taaaataaac ggataaaaac gaataaaaat gaaaaaaagg cacattctag ctgtgaaagg
    16321 atgtgagttt cagatgaaaa tgaaaagcgt aaaagcagtt cttatttttc ttcttctttt
    16381 agcaggtatg gtcacagggt atctgttgtg gagtatgagg gaaccggagt atgaaaaaga
    16441 agggatgctg gtggaaaatg aggtaacggc gtattataac atgacagaaa ccggaattcc
    16501 ggcttttgcg gcagcgccgg ccagtctgct gatagaagaa aatggcgaag ggtggagtga
    16561 aaacctatga gccagacagg cagcagccag agtcagaccg tctatataca gttaaaccag
    16621 atcacggagc tccgccgcag ggatgtattt ttaaaggatg taggggaagt gacctgccgg
    16681 gataaggtga tcttaagtaa atgcagggca ttaaaagtaa agagtatccc tgaaaaagaa
    16741 gcaaggcgtt atgtaatgag cgcgctggaa gtagtgcgga tgttagaggc attggatccg
    16801 tccattgagg taaacaacct gggagagtcg gattttatca tcgcctataa gccgccaaag
    16861 gcttcggaag tttgctggca gtggataaaa acagtctttg tgtgtctggt cagctttttc
    16921 ggggcagcat ttgctattat gacctttaat aatgatgtaa atgtagggga tgtactcagt
    16981 caggtctact ttcaggtcat gggacggcag ccagatggtt ttgggctgat ggaagcagga
    17041 tacagtgtgg gacttgctat tgggattttg gtatttttca accatattgc agtgaaaaag
    17101 ctgaataccg atcccacacc tttggaagtg gaaatgcgcc tttatgaaga aaatatctgt
    17161 aagaccctga tcgaccaggc aggtagaaag gagaaagaga tcgatgtgga ttaaaaatat
    17221 ctttctcctt tttttcggcc tttgttccgg cgggatcatt gcagccggcg tttatgcatt
    17281 tctggccatc atcggtgttt ttgtgcggat gatcgggaaa acgaagacaa agaaaaatat
    17341 catgctctat gaaaatatga tcattctcgg gggagtattg ggaaatatca tggagcttta
    17401 taaggtaaga ctgcccttag gcgaagtgtt tgggcagatc tttcttctta cagggggcgt
    17461 cagcgtggga atatttacag gctgtcttgc catgtctctg gcagaaacct taaaagcact
    17521 tcccacattt tcaaggcgga tccggctgtc tgtggggctt ccatggctga tcattgctat
    17581 tgctgcaggg aaatgtggcg gatcgctgct gtatttctgg aggcggatgg cagcaggatg
    17641 agaatgaaaa aagtggtttc aacatggtca tagaaaggaa aagaacatgg agatcgacaa
    17701 gaaaaactat gcggactaca ttaaagagat caccccggtc cataatcttt gggcttctat
    17761 gggaaaagca tttataacag ggggactgat atgcactctg gggcagttga ttatgaaccg
    17821 ggtgacaaat acctgggggc tggaggcaga ggcggcaggt gcctggactt ccattatcct
    17881 ggtatttttg agcgccttat gtaccggtct taacatttac cagaaactgg taaaattcgg
    17941 cggagccgga gcccttgtac ccatcaccgg ctttgctaat tccgtagctg ctcccgcagt
    18001 ggaattccgc tccgaaggcc aggttttcgg tatcggtgta aagatattta ccatcgccgg
    18061 gcctgtgatc ctgtatggcg tgggagtaag ctggatattg ggaatgggat actgggtttt
    18121 gcagaaaatg tgagtcgcat agacggctca ttttttgtgt gttatactat tgattattaa
    18181 ctatcataaa agtcagaaga gaaggataag gagagaaata catggagcat ctgcgctgcg
    18241 tggtggagcg tattacatat caaaatgagg aaaatggtta tacagtttta aaatgcgcag
    18301 tgaaaaacta tagtgatctg gtaacagtag ttggcactat gccggatacc catgtgggct
    18361 ctgtcctgtc tttagacggc ttctggaaaa tgaacgccag atacggcagg cagttttgcg
    18421 cagagcgctt tgaggaaacg cttcctgcca ctgtatatgg tattgaaaaa tatcttgggt
    18481 ccggtcttgt caaagggatc gggcctgttt ttgcaagaaa gatcgtggaa aaattcggtg
    18541 aaaatacgct ggatattatc gatgagacac cggaagaact gatcaaagtt cccggcattg
    18601 gaaaagtgcg ggcagaacgg atcaaaacca gctggaagga ccagaaagag atcaaaaata
    18661 ttatgctgtt ccttcaaagc aacgaagtca gcacttccca tgctaccaag atctataaaa
    18721 cctatggaag tgaaagcatt tccattgtaa aagaaaatcc ataccgcctg gcagatgata
    18781 tctggggcat tggatttaaa acagccgatt ccattgcgga gaaaatgggt attgacaaga
    18841 caaaatttgt ccgtctgcgc agtggtattt tttatacctt aaataaactg gcggaaaatg
    18901 gtcactgtta tggggaacgg gagcagctgg taaaaaaggc aaaagaactt ctggaagtag
    18961 aagaaggaga aattgaagtt accttagatg agatgcttcg caccaaggac gtgatccagg
    19021 aggaagaagc catttatctg ccgccttatt atttttcaga aagcggttgc gcaaagcggc
    19081 tgctggaact tttgcttacc ggacgaaaat ctgtgattga tgcagaaaaa acactggaaa
    19141 aagtggtatc tcagtcgcag atcacctacg atgagatcca gttagaagca gtaagaacag
    19201 ctatttcttc caaagtcatg atactcaccg gcggtccggg aaccggaaaa accactacca
    19261 cactgggtat tatatctgcc tataagatgg ccggttataa gatcctttta gcagctccaa
    19321 cagggcgggc ggcaaagcgt atggcagagg caacaggtat ggaagcaaag accattcacc
    19381 gccttttgga atacaagcca ccggaaggat accagaaaaa tggggacaag cctttagagg
    19441 gagatgtgct tatcctggat gaatgttcca tggtggatat catgcttatg tacaacctgt
    19501 taaaagcaat tccggaacat atgtctctgg ttttagttgg tgacattgat cagcttccaa
    19561 gtgtaggcgc cggaaatgtg ctccgtgata tgatcgagtc cgggcggatc ccggtggtgc
    19621 gtcttaccag gatcttccgt caggcccagg gaagccgtat tatcatgaac gcccatagga
    19681 taaataaagg ggaagctatt gatatgcggg gcggcagaaa ttccgacttt ttctttgcgg
    19741 aaaagcagac aaacgaagaa actgtggcaa cgctggttca gtattgcaaa gaaaacctcc
    19801 cacggtatta ccatgtagat cctattcagg atatccaggt gctgacgcct atgcaaagag
    19861 gagagtgcgg cgcagtcaat ctaaaccaga tacttcagga agccatgaat cctacagata
    19921 tctgcctgcg ccggggtggt acccagtatc ggttgaatga taaagtaatg cagatcagaa
    19981 acgattatga caaagaagta tttaatgggg atattgggac tatctgcaaa gtggatacag
    20041 aagaacggga actgacggta gattttgatg ggcgaaaggt agtctatgat gtgacggaac
    20101 tggatgagtt gtcattagct tatgcagtca ccatccacaa agcccaggga agcgaatatc
    20161 ccattgtagt catgcccttt accatgagtc actttgtaat gctgcaacga aatcttctct
    20221 atacaggtgt tacaagggcg aagaagatac tggtattgat cggggagcgg aaagccattt
    20281 tttatgccat ccgcaatgaa acaacagcag ggagaaatac aaagctggcg aagcggctac
    20341 agccggaaag tgaagagtcg cgggctgtgg cagcggcact ggcaaaaaaa ctggtccagg
    20401 gagaagaaac tgtgggggct gacggaacag ctttgcaaaa accaaattca gcacaaaaag
    20461 aaaaaatgat cgtatacaaa aacagtatcc agccgtctat ggtatcggaa acaccggccg
    20521 cctacgaagg caacctctgg aaacgccttt cccagtcgaa attccgaagc agtttcacat
    20581 taaaggctaa tgacaggcat tatgtaatag aaaaaggtat ggatacagtg cgaagccatg
    20641 ccacagattt tatccgtgac cggttggcac ctgcagagcc gaaaaacgac ggaaaacaga
    20701 cgccaatgag aggtcatcct gtatttatcg ggcaacacgc taccggtacc tgctgccgca
    20761 gttgtctgga aaaatggcat cacattccaa agggaagaga actgacagaa acagagcaaa
    20821 aatatgtggt agatgtgatc atggagtgga tcaaaaggca gatgcagacc ttataagaga
    20881 gttgtatgaa atggttacaa atctggatgt tttgcagctt tcagatggag aaaaatgcac
    20941 actggcttta tttggagata ttgcacgacg cttggcgatt gcaaatccgt cattggatga
    21001 tccacttcag ggagatggtg tggttctaat cgatgaattg gaacttcata tgcacacttc
    21061 ctggcaacgg aaagttattt ccatgttaaa gaaaacattt cctaatattc agtttatcat
    21121 tactactcat tcacctcaga tcctggggga ggtagatcac gattttaatg tttatgcttt
    21181 atccaggaac ggatcggata ttcaggtggt acaaacaacg gggatgtttg gtatggattc
    21241 caattctatt ttggaagaca aaatggaaac agattcggta agtgcgatca ttaaacaaaa
    21301 aacagaggaa atgtattcct tgcttgaatt gaaagacttt gatggtgcgg aaaatattgc
    21361 agatgagatt gacgaattga ctcagaacag aaatatagat acagttcgtg caagggtaat
    21421 tatcagaaga ggtagataca agaataacga tttatctaat ccgtaacgat gaacagaata
    21481 tttcgttatg cgggtgtgca ttttttatgc gattttatag cttgttcata atatctaaca
    21541 ggtgctgata gctgtccaca ctgtcgtaaa ccacatctgc agaggagaaa ttgtgaaaca
    21601 gctttctggc acagtcgatt ttcgcttttt caataggctt caaatttaat gtttccatgg
    21661 gagcatacac ccagccctcc ggatttcaga gaatcggaga gcttaatatt attgcgagcc
    21721 tggatacggt gaatattatc caaaagctga ccgtaatcga gctgtacctt agcgttggca
    21781 aagcccatgt acagttcttc atcagaaggc atccagtttg tcacatctcg cttgatttca
    21841 acatcgcggc ggtaggtgaa gtggtcgttg aaaggctgac cagcaaatac acctaccacg
    21901 ctgtccacag cctcggtctg gtaaggctgt atggtaaatt tgaatttcat ctcagccatt
    21961 acagcacctt cctttgcgtt ctcggactat aggtttcaaa tatctgctca aagtttgtgg
    22021 ctacgctgtc actcgccata ctgctgtcac ggaatacggc ataaactggc tgcttctgcg
    22081 caatggcttt gacggtttct tctgtaacat ccttgtcaaa gcaggcaatc agataaccat
    22141 ccgctacaga gaacaccttc ttgccagcaa tttctgtttc ctgaatgtca gaggagagca
    22201 aaataccaag gtcgagcatt acctggaaga gcagatcttc tggtgtacga tccggtttga
    22261 tattgtcagc gaacagttcc atctgtccct gattgtaatc ggcagggcgg tagtagacat
    22321 cttccatatt actgctgtct attttgaaaa cgcggaagcc gatatcaaga ttagtagtgg
    22381 taagcggatt ttcttccttg atcttctttc cagcacgacg gatacgctct tcaccaattt
    22441 cacaaatatt actatattgt gcattttgaa tagtttctgg tagttgaacc ataataaatt
    22501 ttcttgaact gttatcggtt aggttccagg atttgccaac aataagtgct gaacgtgatt
    22561 tttatttgag ttagatacat tatttaaaaa gaatgagaat ccacaatatg gatgaatgtc
    22621 atttttaggt ttaaagaaat tactggaaga gggaacttga taatggctaa aattgcacat
    22681 tttcccaaac aaaatcaccc ttaaaacccc gcataaactc tgattgtatg caccaaaaaa
    22741 caaacattgt tttttcttta acacaagata aaatttatca aaaaatcact tgcctttctc
    22801 cgaaaatatg atatcattcg tggtgagtta agaaaaacta acaatcgcgg aatacaaaaa
    22861 caggaggaag gaaatgttta actacgaaca gtgggacgga tttgaaggtc gtctctggaa
    22921 agaagagatc aatacaagag atttcattca gaagaactat acaccatatg acggtgatgc
    22981 atcattctta gcaggaccta ccgaagctac ggataagctt tggggaattc tgcagggact
    23041 tcagaaggaa gagcgcgcta agggcggcgt tttagatatg gatactgata tcgtatctgg
    23101 tattacttct catggacctg gatatattag tgaagagaca aaggacttag agcagatcgt
    23161 tggtctgcag acagacaagc cattaaagag agcattcatg ccttacggtg gtatccgtat
    23221 ggcagaggaa gcttgcaaga actatggcta cgagccaagc ccagagcttc acaagatctt
    23281 cactgagtac tgcagaaccc ataaccaggg cgtatttgat gcttacactc cagagatgaa
    23341 gaaagttcgt cacaacaaga tcattaccgg acttccagat acttatggcc gtggacgtat
    23401 cgtaggtgac taccgccgtg tagctctgta cggtatcgac cgcctgatgg aagagaaggc
    23461 aaatgatttt gctaactgcg gtgacggaac tatgactgat gacgtgatcc gtttaagaga
    23521 agagatctcc agacagtatc aggcattaaa ggatatgaag aagatggcag catcttacgg
    23581 atacgatatt tcccagcctg ctaagactgc taaggaagct gttcagtggt tatacttcgg
    23641 atacttagct gcagttaaga cccagaacgg tgctgcaatg agcgttggtc gtatctctac
    23701 tttccttgat atctacatca agagagatat ggataagggc atcttaactg agtcccaggc
    23761 tcaggagctg atcgaccaca tggtaatgaa gttccgtatg gttaagtttg caagaatccc
    23821 atcttacaac cagctgttct ccggcgaccc ggtttgggct actctggaag ttggtggtat
    23881 cggcgtagac ggacgttcta tggtaactaa gaccgactat cgtttcctgc acaccttaga
    23941 gaacatggga ccatccccag agccaaacat gactgttctt tattcttctg cactgccaga
    24001 gaacttcaag aagtatgcag ctaagatctc tattgataca agctctgtac agtatgagaa
    24061 cgatgacgta atgaagccag agtggggcga tgattactcc atctgctgct gcgtatctgc
    24121 tacccagacc ggtaaggaaa tgcagttctt cggtgcccgc gcaaacctgg ctaagtgctt
    24181 actgtacgct atgaacggtg gacgtgatat gaagtccaaa gagcaggttg gtccagagta
    24241 cacaccaatt acttctgaat accttgatta tgatgaagtg atccgtaagt acgaggtaat
    24301 gatggattgg ctggtaggcg tatatgtaaa taccttaaac ctgatccagt atatgcatga
    24361 caagtacttc tatgaggctg ctgaaatggc tctgatcgat accgatgtca gacgtacatt
    24421 cgctaccggt attgctggtt tctctcatgt agttgactcc ttaagtgcta ttaagtacgc
    24481 aaaggttaag actgttcgtg atgagactgg cctgatcgtt gacttcgtaa ctgagggaga
    24541 cttcccaaga tacggtaatg acgatgacag agcagatgag attgctgtat ggctgttaaa
    24601 gaccttcctt gagaagttaa agagacgtca cacctacaga aattctgagc ctacaacttc
    24661 tatcctgacc attacttcca acgtagtata tggtaaggct accggaagca tgccagatgg
    24721 cagaagagca ggcgagccac tgtccccagg tgcaaaccca agctacggcg cagagcagaa
    24781 cggcttactg gcttccttaa actctgtagc taagctgcct tacgagtggg ctctggatgg
    24841 tatctccaat acccagacta tcaacccaga tgctttaggc cacagcgaag aagaaagagt
    24901 agataacctg gtacaggtat tagacggcta cttcgatcag ggcgctcatc acctgaatgt
    24961 aaacgtattt ggtaaggaga agctgatcga tgctatggag catccggaga aggaagagta
    25021 tgctaacttc actatccgtg tatctggata cgctgttaag ttcatcgact taacaagaga
    25081 gcagcagatg gacgttatct ccagaacctg ccatgcaacc atgtaattta atacaggctg
    25141 catttaatgt aactgagaaa taaaatagca ttccgctatt gatcaaagat ccatataatg
    25201 atgtaacggt tcaggacatt atgttctgga ccgttataaa tatttatgga atgcattttc
    25261 agaaaataag gagaaagacg tggaaggatt tgttcattct atggagacct ttggactggt
    25321 tgacggtcca ggtgtccgtt tcgttgtatt tcttcagggc tgccgcatgc ggtgccgtta
    25381 ctgccataac ccggagacat ggaaattaga gggaggtact ccctggactg cagataaatt
    25441 attttcccat gtataccggt ataagagcta ctggaataaa aaaggacagc caaacggcgg
    25501 tattaccgtc agcggaggag aacctcttct ccagatcgac tttgttacag agttttttaa
    25561 gaaagccaag gaaaaaggcg ttcacacagc tctggataca gcaggcaatc cttttacact
    25621 ggaagaacca tttatcagca aatttgaaga actgatgaaa gtaacggatc tggtgatcct
    25681 tgattttaaa gagatcgaca acgaaaaaca caaaaaatta accggatgtc caaacgataa
    25741 tatcgtacag atggcaaaat ggctgtcaga ccacggaaaa gatatgtggc tgcgccatgt
    25801 acttgtgccg gatctgaccg atgacgaaga aggactggta agaacccgcg atttgatccg
    25861 ggaattaaag acagtcaaac gtgtagaggt gcttccatat catacactgg gactgtttaa
    25921 atggcagaaa ctgggaattc cttatacttt ggatggtgtg agaacaccta ctgcagaaga
    25981 agtaaagaat ttcgtgttga tcttggaagt gtgggaagag aaaaaggggc aattggtaaa
    26041 agaggttaat ggtttc
//
LOCUS       DBMDUA010000015        25419 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1327_length_25419_cov_4.043605, whole genome shotgun sequence.
ACCESSION   DBMDUA010000015 DBMDUA010000000
VERSION     DBMDUA010000015.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 25419)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25419)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25419
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1327_length_25419_cov_4.043605"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(104..487)
                     /gene="gloA2"
                     /locus_tag="ACZPCL_02385"
     CDS             complement(104..487)
                     /gene="gloA2"
                     /locus_tag="ACZPCL_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMU1112c/YaeR family gloxylase I-like
                     metalloprotein"
                     /protein_id="HIR1161370.1"
                     /translation="MILSKIHHIAIIVSNYETAKDFYVNKLNFPIIRENYRPERNDWK
                     LDLRVNEYTELEIFAEPDPPKRVNRPEACGLRHLAFCVESVEQTVKELAEVGIDCEPI
                     RVDAYTGKKMTFFQDPDGLPLELHE"
     gene            complement(946..2394)
                     /locus_tag="ACZPCL_02390"
     CDS             complement(946..2394)
                     /locus_tag="ACZPCL_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024832.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HIR1161371.1"
                     /translation="MKSQTSIKKNFIYQMIYQILIIILPFVTSPYIARTIGAEGLGTY
                     SYSYSIAYYFVLFSMLGLANYGNRAVAQCRDNQKNLNETFSNIVTLHIAFSFICCVVY
                     ILYVFFLANEKLYAGIQFTYVLSGLFDVSWFYFGIEKFKLTVTRNIIIKILNVVCVFV
                     FVRNANDLWKYCAIMAVGMLISQISLWFPLNKFVAFQKPTWKKMKPHIEPLLILFIPA
                     IAVSLYKYMDKIMIGAMSNKTQLGFYENAEKVINIPLTIITSFGTVMLPKMSNLVASH
                     NREKTNQYISLSMKYVMCLSFALAFGLAGIGKVFAPVFWGQEFMLSGVLIMGLSITIP
                     FMSFANIIRTQFLIPSEKDKEYLSSVIGGAVVNLIANTLLISKIGAIGATIGTILAEI
                     FVCVIQTLSVRKELPVLRYIKSIWMFAVFGVAMFVGVFYIGNALENKVSTLFLQIICG
                     MLIYGTFCVIYFVHTKDEMILKMLRGIKGKIR"
     gene            complement(2453..2644)
                     /locus_tag="ACZPCL_02395"
                     /pseudo
     CDS             complement(2453..2644)
                     /locus_tag="ACZPCL_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576470.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
     gene            complement(2665..2826)
                     /locus_tag="ACZPCL_02400"
     CDS             complement(2665..2826)
                     /locus_tag="ACZPCL_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161372.1"
                     /translation="MLKFLLEDNCSIVVRPSGTEPKLKTYISVSAENKEAAEKVEAAI
                     RKSAEEYLK"
     gene            complement(2886..3728)
                     /locus_tag="ACZPCL_02405"
                     /pseudo
     CDS             complement(2886..3728)
                     /locus_tag="ACZPCL_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014877.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
     gene            complement(3737..4984)
                     /locus_tag="ACZPCL_02410"
     CDS             complement(3737..4984)
                     /locus_tag="ACZPCL_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR1161373.1"
                     /translation="MKNYKIAVAGTGYVGLSIATLLSQHHKVTAVDIVPEKVEMINNR
                     KSPIQDDYIEKYLAEKDLNLTATLDAKAAYSDADFVVIAAPTNYDSQTQHFDTSAVEA
                     VIKLVMESNPNAIMVIKSTIPVGYTASAREKFNSKNIIFSPEFLRESKALYDNLYPSR
                     IIVGTDLNDERLVEAAHEFAGLLQEGAIKENIDTLFMGFTEAEAVKLFANTYLALRVA
                     YFNELDTYAESKGLNTQQIIDGVCLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYAD
                     VPENLIEAIVESNRTRKDFIADRVLKLAGYYGYDAENEFDEDREKAVIIGVYRLTMKS
                     NSDNFRQSSIQGVMKRIKAKGATVIIYEPTLKDGETFFGSVVVNDLAKFKEMSHAIIA
                     NRYDKCLDDVKEKVYTRDIFQRD"
     gene            5376..6026
                     /locus_tag="ACZPCL_02415"
     CDS             5376..6026
                     /locus_tag="ACZPCL_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161374.1"
                     /translation="MGEDENKKRERRHQEDLERLRGFRPIDDTFMRGLFKENIPLAEL
                     VLRIITGKPDLVLLKCETQADMKRVTGARSVCLDAYATDSTGKKYDIEVQRSDNGADP
                     HRARYHSSMLDIEYRDNSDIGRLMHDFNCTNASDMYFDLLAEKTRYLKENPKGVNEMC
                     KVMEDLRNESYAEGREEQAKSTAIKLNQKGLPIEDIADSIGFSIETVKNWLTPKAV"
     gene            6062..7309
                     /locus_tag="ACZPCL_02420"
     CDS             6062..7309
                     /locus_tag="ACZPCL_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR1161375.1"
                     /translation="MKNYRITVAGTGYVGLSIAVLLSQHHKVTAVDVVPEKVEMINHR
                     KTPIQDEYIEKYLAEKELDLTATLDAREAYKDADFVVIATPTNYDSRTQHFDTSAVEA
                     VIKLVLEYNPDAIMVIKSTIPVGYTASIRKKFGSKNIIFSPEFLRESKALYDNLYPSR
                     IIVGTDLKDPHLTEAAHIFAGLLQEGAIKEDISTLFMGFTEAEAVKLFANTYLALRVS
                     YFNELDTYAESKGLNTRQIIDGVCLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYAD
                     IPENLIEAIVESNRTRKDFIADRVLKLAGYYDYDEKFEFSADKEKEVTIGVYRLTMKS
                     NSDNFRQSSIQGVMKRIKAKGAKVVLYEPALKDGETFFGSRVVNDLQKFKDMSQAIIA
                     NRYDKCLDDVAEKVYTRDIFRRD"
     gene            7438..7896
                     /locus_tag="ACZPCL_02425"
     CDS             7438..7896
                     /locus_tag="ACZPCL_02425"
                     /inference="COORDINATES: protein motif:HMM:NF016763.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR1161376.1"
                     /translation="MVSFTFTYRVYKREKYPLQNSAIWTLFLCYWLVLIQTAFFSRES
                     GSRKQISLILFETWGHSVQAHAYFIENIFMFIPFGILAPVLFKRMKKMYWCVLAGFLM
                     SCTIEISQLLTQRGYMQIDDVLTNTAGAMAGWLIWKIVTLLTGFLKSEFT"
     gene            7956..9284
                     /locus_tag="ACZPCL_02430"
     CDS             7956..9284
                     /locus_tag="ACZPCL_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1161377.1"
                     /translation="MDNDYLIKKPPLQALFLFALPMIIGNLFQQTYTMVDSAIVGRYV
                     SEQALAAVGASYALTNIFICVAIGGGIGASVIVSRYFGAKEYGRMKTAVFTALLSFLG
                     ISLVLGIAGLILGKQIMIWLNTPADCLDMASEYLRIYFLGLPFLFMYNVLSAMFNALG
                     KSRIPLYFLIFSSIFNVILDIIMVTKMDMGVAGVAWATLIAQGISAVLSFGVLLKNLK
                     GLEGKQKTLFDKMELAEMTRIAIPSILQQSTVSIGMMLVQSVVNGFGSEALAGFSAAM
                     RIESICVVPMSAIGNAVSSFTAQNIGAERKDRVVMGLHAASKMVIVCSVVICFFLEVF
                     SHSMIMLFLGNEGTQIALFTGIGYLTFMGWFFCFIGFKMAVDGLLRGAGDMKMFTVAN
                     LVNLGIRVIIAVTCAPRFGIAMVWYAVPVGWFANWIISYAEYRTGKWKTL"
     gene            9342..10742
                     /locus_tag="ACZPCL_02435"
     CDS             9342..10742
                     /locus_tag="ACZPCL_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161378.1"
                     /translation="MERYLKFREEYSRFFYRGYDVEETLNELKITYTFEIEGLSVFTP
                     TWTFPKNGNPEAKWMEDPLMEKMIFSLGMVELVSYWKITCSPQVVIEAGSLSEEQILW
                     WKDLYFNGLGEFFYVNKITEADPEGFMEIVCPLSEEESAANSYEKKQDTAGAEQGVLV
                     PVGGGKDSAVTLELLKGADVPVFAYIINPRGATIHTTESAKLTADHVISVHRTLDKRM
                     LQLNKEGFLNGHTPFSALVAFSSVIAARMAGLSYIALSNESSANESTVQGSTVNHQYS
                     KSFKFEEDFHQYQTDHLPGSAYYFSMLRPLSEFQIAKYFAKQKQYHKIFRSCNAGSKT
                     DSWCGHCPKCLFVYLILSPFLSREEVKEIFGTDMLNDPDMQPTLEQLVGIQEEKPFEC
                     VGSRDEINTAIVMTIDNLERSGKELPYLLKYYKTTGLYEAYKAKGDQFSSYFDKQNLV
                     PTPWEKLVEKNCKDEA"
     gene            10747..11094
                     /locus_tag="ACZPCL_02440"
     CDS             10747..11094
                     /locus_tag="ACZPCL_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161379.1"
                     /translation="MMIERIRPWVQGKKVLILGFGREGKSTWNVLKKLDCCSFVDVAD
                     MAEPKEKPEGIRNWIAGPDYQKCLNEYDVVFKSPGVVLEKPVQSYSSQILSQTELFFQ
                     CFRDQIIGITGVP"
     assembly_gap    11202..11211
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11212..12294
                     /gene="murD"
                     /locus_tag="ACZPCL_02445"
     CDS             <11212..12294
                     /gene="murD"
                     /locus_tag="ACZPCL_02445"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR1161380.1"
                     /translation="PFAKGKSTITTLIYHLLKEAGMDTILVGNIGIPVFDHMEEVGED
                     TKIVCELSCHQLEYMTVSPHIGILTNLHEEHLDHYGTLEKYIQAKRHIYLNQKEEDVM
                     ICNVQCLPEPGTCKSKLIAAKPSFEEPVFQILGVPGQGAVSEEMVNIWKKMEVIQEND
                     TTEARYDGHSIEIPVSQIRLLGQHNYFDIGIAWAVCRLLNIRDEVFLEGLKTYEPLPH
                     RLQFLGEKNGIKYYDDSISTICETTIQALNTLKDVDAVLIGGMDRGIDYSELIRFLSG
                     HPVPYIILMEATGKRIFEEIRQDYPDFQKMERLILTDHLEDAVKEAQKLTRPGHSCLM
                     SPAAASYGIFKNFEERGEVFARLVLK"
     gene            12400..12861
                     /locus_tag="ACZPCL_02450"
     CDS             12400..12861
                     /locus_tag="ACZPCL_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1161381.1"
                     /translation="MKGNNVGTYDTLNDVLVNLFRDINSIEENAITSQEYKDLTNNDM
                     HVINAIGVGTTKNMSSIAKELSVTVGTLTISMNSLVKKGYVTRCRSTQDRRVVHISLS
                     EKGVKAYYHHKHFHEQMIQGVVEVLTEEELQALIKALTKLNSWFRSFGKDE"
     gene            12877..13428
                     /locus_tag="ACZPCL_02455"
     CDS             12877..13428
                     /locus_tag="ACZPCL_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HIR1161382.1"
                     /translation="MKKWLLGLLTAMMAVSLAACTPTTEKKQETTTTAAAAKQEQIEA
                     QTTGGASDKVPDPNVAPVAIISVYHKGDGDSLVQDMDSLDNDELDPQALVSKMTEYGI
                     FTEGTQVLNFNITGEGDVKKAVLDLNKVENDEGVSDSVFLVEIGNTFIENFELSSLTL
                     KENGADLAGATDLTYVADYENVD"
     gene            14271..14714
                     /locus_tag="ACZPCL_02460"
                     /pseudo
     CDS             14271..14714
                     /locus_tag="ACZPCL_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577112.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6128 domain-containing protein"
     gene            14837..15424
                     /gene="tadA"
                     /locus_tag="ACZPCL_02465"
     CDS             14837..15424
                     /gene="tadA"
                     /locus_tag="ACZPCL_02465"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR1161383.1"
                     /translation="MDEAAQKGLVTRQRRWEARNEKRRQKALEEEAKAARIKAEDEKY
                     MKEAIKQAKKAAKIGDVPIGCVLVKDGEIIARGYNRRNADKTVLSHAEVTSIKRACKK
                     EGDWRLEDCTLYVTLEPCPMCAGAIVQARIPRVVIGSMNSKAGCAGSVLNLLKEPGFN
                     HQAQVVTGILEEECSEMMSSFFTELRKKKRLPETP"
     gene            complement(15381..16757)
                     /locus_tag="ACZPCL_02470"
     CDS             complement(15381..16757)
                     /locus_tag="ACZPCL_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1161384.1"
                     /translation="MSHTEKKHDQYYEMANAPISHIIPKLAAPTIVSMLISSIYNMAD
                     TFFVSQLGTSASGAVGVIFSAMSIIQALAFMIGMGSGNYMTRSLGAGKRELAEQIVSI
                     AFFTGIIMGLVITVSATFNIHRVVLLLGATETIAPYAEAYAHYIFIAAPFMICSFIMN
                     NLLRFQGKASFAMVGITVGGLLNIALDPLFIFTLDMGTAGAALATGLSQFISFCILLW
                     MCNHQDSCISISIRKFRPKLAIYREVLYGGIPSLGRQGIASVATILMNFMCQPYGDAA
                     IAAMSIVSRFMFFINSVIIGFGQGFQPVCSYCFGAGLYDRVKKACWFCIRVSTIILLV
                     LSVISFPAARSIITLFRRNDPTVIEIGTLALRLQLITLPLQGAVIMGNMMPQSIGYGF
                     RATLVSTARQGIFLIPLLTIMTYTIGLLGIQCAQPLADIATFILSLFVIRGIFKEFPE
                     DASSFAAQ"
     gene            16890..17151
                     /gene="ffs"
                     /locus_tag="ACZPCL_02475"
     ncRNA           16890..17151
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACZPCL_02475"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            17434..18513
                     /locus_tag="ACZPCL_02480"
     CDS             17434..18513
                     /locus_tag="ACZPCL_02480"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1161385.1"
                     /translation="MRRIGILTSGGDCQALNATMRGVAKSLYNMLDDVEIIGFEDGYK
                     GLMYADYQIMKPADFSGILTQGGTILGTSRQPFKQMRVPDENGLDKVEAMKHTYKKLK
                     LSCLVVLGGNGSQKTANMLSEEGLNVVSLPKTIDNDLWGTDMTFGFQSAVEVATNAID
                     CIHTTAASHGRVFIVEVMGHKVGWLTLYAGLAGGADIILLPEVPYDIQVVCDAITKRT
                     KAGKRFSILAVAEGAISKEDAAMSKKELKEKKKNGVVYPSVAYEIGAKIQEVTGSEVR
                     VTVPGHMQRGGEPCPYDRVLATRLGAAAAELIINEEYGYMAALKNNEITKVPLSEVAG
                     KLKTVDPNCSMIKEAKRIGISFGDQ"
     gene            18532..20217
                     /gene="dnaX"
                     /locus_tag="ACZPCL_02485"
     CDS             18532..20217
                     /gene="dnaX"
                     /locus_tag="ACZPCL_02485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR1161386.1"
                     /translation="MSYTALYRKWRPVSFEDVKGQDPIVQTLKNQITSERIGHAYLFC
                     GTRGTGKTSIAKIYARAVNCEHPVDGSPCNQCPSCKSILAGTSMNVVEIDAASNNGVE
                     NIRDIREQVQYPPTEGKYRVYIIDEVHMLSIGAFNALLKTLEEPPSYVIFILATTEVH
                     KIPITILSRCQRYDFKRISLETIAGRLRQLTEEEHIEVEDKALMYVAKAADGSLRDAL
                     SLLDQCVAFHYGKLLTYENVLDVLGAVDSGVFSSMFNAVIEGRTKDCITQLEEIVIQG
                     RELGQFVTDFIWYLRNLLLVQSADDAEGLLDMSEENLKQLKEDSTKTDGNTLMRYIRI
                     FSELSNQLRYASQKRVMVEVALIKLTRPSMEPDLDSVLQRLKQLEAAVEDLEAGGFRP
                     GTMVTAPMGAVPVTAGVQPQISGTQGQMAVPVQGGPTGNPPETAGAAGITPPNQVSLP
                     PAQLEDLNLIRNEWAKIIRSAGGSARACFRDTVVEPGGEGCLTIVFLDSMSYDMGRRP
                     TVIGQLEQLVQANYGKSIYFKTRLAGRGERLDTIYVTKEDLEDKIHMDITYED"
     gene            20260..20616
                     /locus_tag="ACZPCL_02490"
     CDS             20260..20616
                     /locus_tag="ACZPCL_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR1161387.1"
                     /translation="MAKRGGFPGGMPGNMNNLMKQAQRMQRQMEETTKELEEKEYTAA
                     AGGGAVTVTVSGKKEVVSIKLSEEVVDPDDIEMLQDLIVAATNEAFRQMEAANSEAMS
                     KLTGGLGGALGGGFPF"
     gene            20616..21212
                     /gene="recR"
                     /locus_tag="ACZPCL_02495"
     CDS             20616..21212
                     /gene="recR"
                     /locus_tag="ACZPCL_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR1161388.1"
                     /translation="MDYYSSQITKLIEELSKLPGVGAKSAQRLAFHIINMPKEQVDEL
                     AGAMTGARNNVRYCKECFTLTDKEICPICSSDKRNHKVIMVVENTRDLAAYEKTGKYN
                     GVYHVLHGAISPMLGIGPNDIKLKELMHRLQSDVEEVIIATNSSLEGETTAMYISKLI
                     KPTGIKVTRIASGVPVGGDLEYIDEVTLLRALEGRTEL"
     gene            21247..23376
                     /locus_tag="ACZPCL_02500"
     CDS             21247..23376
                     /locus_tag="ACZPCL_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1161389.1"
                     /translation="MDKYEFNIKVEQIKKLVNKSDYETAMKIADTIDWRRVRNVNILS
                     MVAGIYEKNGEFQEAKDILLLAFERAPIGKRLLYKLADLALKEGNVEEAEDYYKEFCN
                     LAADDPRQYILRYMILKAKGAPVEQLIHTLEQYCNAELDEKWMYELAELYNQAGMEQL
                     CVMTCDKIMLMFGLGKYVDKAMELKLQYAPLNQYQMDLVENKEKYEAKLKAVEQEYKK
                     GTSIVQPIENTDRRQLDREEEPVEDTAEDETVYAEDEYTEDYREEPAVTIQDDDITEE
                     EPVSEEPEEELAEEPEETYETEEPETEDYEAGESEAEESEEEPEADEEQAVYETAAAD
                     EEIRASIHEARAQENLAREMSKLSAAAEEEDLPEDEMGQTRALTDIRELRKGPVYTGS
                     NHLMIEAEVPEKGLALAVEALRKIHKETGAKNQAAKISGEKLNRRGVFALADKLTGKD
                     LIIEQAGDMNESILQELNQLMARDETGMNVVLIDTARRLEELHRVYPGLAKRFECIGT
                     SDSIKAKKAAAPVPSESGKGSLAKAPEVVEKKVQPAPKPVAAPKEAPAPKTAATKAVV
                     TSKAAAPKPAAKPAAQPEKKAAAPAKKRPVQAPAVKEPARMAETEQPEHSEQEMDIDE
                     FAKYACQYASKIDCSISGKSMLALYERIEIMEEDGIALTREEAENLIEEAADRAENPS
                     FFKRLTGIFSSKYDKDGLLILKEEHFL"
     gene            23373..24212
                     /locus_tag="ACZPCL_02505"
     CDS             23373..24212
                     /locus_tag="ACZPCL_02505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1161390.1"
                     /translation="MRNIKLIALDLDGTLLNMEKKVPQGNYQALKECEKAGIQIVPAT
                     GRGVGGIPPMIRELPGANYAITTNGAVVADLKNNKAIKTCGLSNEMIQRILNIAKKYH
                     SATDPFIDGRAITEPASIDHMDEFGLSPEMQKLIRDTREVVPNVMEYVKTTGAEAEKV
                     NIFMADLEEREVLRKELMAIPELSISSSMYNNLEVNAKGADKGSALLWLADYLGIDRE
                     ETMSFGDGENDIPMIQAAGIGVAMENALDTVKAAADMITLKNDEDGVAAAIRKIIFGS
                     NEK"
     gene            24209..24925
                     /locus_tag="ACZPCL_02510"
     CDS             24209..24925
                     /locus_tag="ACZPCL_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR1161391.1"
                     /translation="MMTEHWLSIAAGVFLVGMMLYGHYRGLLRQCVSLGALVLTIITV
                     KVATPYVTDFVKANPVIRENAAHFIMEVSGWQPPEESESILPAAQRMAIEELKLPQSV
                     KDVLLENNNSEFYRLLGVDQFGEYISTCLSDMLINTLGSVLVFAASYIVIHLVIRWLN
                     LLSRLPIICGLNQMAGGIVGLAEGFLLLWLAGFILSFFTETPMGQMLEAQVNSSIWLS
                     LIYRYNLMNVILGSIINGIL"
BASE COUNT         7740 a         4949 c         6081 g         6639 t
ORIGIN      
        1 acttcatcca gaggttgcaa tagcataaag cactattttg ttagctgaaa tattgaattt
       61 tcaaggtgcg aagccctgtt taggtgtggg aagttttagt ttattattca tgcagttcca
      121 atggcagacc gtccggatcc tggaagaagg tcattttctt gcctgtgtaa gcatctacac
      181 gtattggctc acaatcaata ccaacttctg ccagttcttt taccgtctgt tcaacactct
      241 ccacgcaaaa agccagatgg cgaaggccac atgcctccgg acgattcaca cgctttggcg
      301 gatccggttc tgcgaagatt tccaattctg tatactcatt cactcgcaaa tccagtttcc
      361 agtcgttgcg ttccggacgg tagttttcac ggataattgg aaaatttagt ttgtttacat
      421 agaaatcttt ggcagtttcg tagttggaaa cgatgatggc aatgtggtgg attttggata
      481 ggatcatttt taggggctcc tttagaggta tttttgttgt gaactgtatg atggggttaa
      541 aatatgccgt gtggacatca gaaaagcagg ttgtccatac ggctatgttt tgaatatttt
      601 cttatttcta taattactaa acttaccata tatggaatta cacaatgtgc aagaaaatta
      661 gaaggttgct tttttatact gcttccagag gaagtttagc cattgcttct ttaaactgtt
      721 caactgtcag ctcttcccat cggctttcac ctctctcttt cattgctgat aggagtacaa
      781 ataatcctac tatattttca tgttatcata aagctaacct tttatcaagc agcctacctg
      841 tgaacgtttt cagcttcaga tcaaagtgtt cgcagttata tgattgtcga aagtcctggt
      901 ttggttcgtt ccaacaatat gcgtgaaata agcccgttcc agatattatc tgatttttcc
      961 ttttatcccc cttagcattt ttaaaatcat ctcatctttt gtgtggacaa aatatatcac
     1021 gcaaaatgtt ccatatatca gcattccaca gatgatctgc aggaacaatg ttgacacttt
     1081 gttttccaga gcatttccga tatagaacac ccctacaaac atagccactc caaaaacagc
     1141 aaacatccat atacttttta tatatcgtag taccgggagt tcctttctaa cgctcaacgt
     1201 ctggatcaca caaacaaaaa tctcggcaag tattgtacca atcgttgctc caattgctcc
     1261 aatttttgaa atcagtagcg tgttcgcaat tagattaact actgccccac caataacaga
     1321 agaaagatat tctttatcct tttctgatgg aatcaaaaac tgtgtccgaa tgatatttgc
     1381 aaacgacata aaaggaatcg taatcgaaag acccataatc agcacacctg aaagcatgaa
     1441 ttcctgcccc cagaaaacag gggcaaaaac ctttccaatt ccagccaaac caaatgccag
     1501 tgcaaaggac aaacacatca catatttcat tgacaacgat atatactgat ttgtcttttc
     1561 cctattgtga gaagccacca gatttgacat ttttggaagc attactgtcc caaatgacgt
     1621 aatgattgta agtgggatat taattacttt ttccgcattt tcataaaacc caagttgagt
     1681 cttattactc atcgcaccaa tcatgatttt atccatatat ttatataaac taactgcaat
     1741 ggctgggata aataagatca aaagcggttc gatatgcggt ttcatttttt tccacgtcgg
     1801 cttttgaaat gcaacaaatt tatttaaagg gaaccataaa gaaatttggc ttatcaacat
     1861 gccgacagcc ataattgcac aatacttcca taggtcattc gcatttctaa caaaaacaaa
     1921 tacgcaaaca acattcaata tttttataat gatatttctt gttactgtca atttaaattt
     1981 ttctattcca aaatagaacc agctcacatc aaataagccg ctcagcacat aagtgaactg
     2041 aattccggca tacagttttt cgttggccag aaaaaacaca tacaaaatat acaccacaca
     2101 gcaaatgaaa gaaaaagcta tatgtaatgt cacaatatta gaaaatgttt cgttcaggtt
     2161 tttctgatta tctctgcatt gtgcaactgc ccgattccca tagtttgcaa ggccaagcat
     2221 ggaaaaaagc acaaaataat atgcaattga atagctgtat gaatatgttc caagtccttc
     2281 agcaccaatt gttcttgcaa tataaggcga tgttacaaaa ggaagtataa taatcagaat
     2341 ctgatagatc atctgataga taaaattctt tttaatactg gtttgacttt tcattttatc
     2401 tttctctctt tacgataaat acttttcatt ttctattgtc tccaacgatt gcctgtgatt
     2461 tgctcgttgc aatcgtgatc tttgcatcag catccaccgt ggtgatctgg cgatacacac
     2521 gctgcaccat agactcatac tcatcattat tcttgaacag cttgatgaac tgcttgctac
     2581 gcacatcatt agaaagaggc caaagacgct tgcctgaacc accagaaagt aaaataatat
     2641 tcatatgttt ttctcctctg ctctttactt caagtactct tcagcactct tgcgaatcgc
     2701 cgcttccacc ttttcggcag cttctttatt ctctgcacta accgagatat atgttttgag
     2761 cttcggctcg gtaccggaag gacgcaccac gatggagcag ttatcttcca gcaagaactt
     2821 gagcacatcg gacttcggca gaccatccag accgggtgca taatccaaca gcttgcctct
     2881 ttgatctccg ggttgggata cgggcaggtc gggaagttgc cgtccggctg ctcctgctct
     2941 ttgaccaccg tgatgttggt gtagcccatt tctttcaaag tgcgggtcac aggcttcaag
     3001 ccggtaccat tcagcgggct atacacgatg gctacatttt tattaacttc ttcgccaaag
     3061 agaacagact ggctcttgac ctgctccacg aatgcggtgt agacctcgtc cggaatgtac
     3121 tggatgctgc tgtttgccac acctgtttca aagtcgctgg tcttgacgtc tgcaaaaatg
     3181 tctagcttct caatctcggc aaggatctcc gttgcagctt cggtagtgat ctggcagccg
     3241 tcagcaccgt agaccttata accattgtac ttgctgggat tgtgagatgc ggtcaccatg
     3301 acaccggccg aggtgtgcag ataacgggtc gcaaaagaca cggtcggaac cggcatcagg
     3361 acgggccaga tatttacctg gacaccattc gctgcaaaca cgcctgcggc gacttttgca
     3421 aacacatcac tcttgatacg gctgtcataa ccgattgcca cagacggctc tgtgaagttc
     3481 tttttcagat aatcagccag accctgagaa gcctttgcca cggtatagat gttcatgcgg
     3541 ttagtacctg caccaattac accacgcaga ccacccgtac caaacgcaag gtcacggtaa
     3601 aatgcatctt caatttttgt atcatccagc gttttcagtt cggcgacaac atctgcatct
     3661 gtggttgcgt tttccaacca acactcgtat tcctttttaa tatccacaat aatctcctca
     3721 tcttccactt atgttcttaa tcacgctgga aaatatctct cgtataaact ttttctttca
     3781 catcatccag acacttgtca tagcgatttg cgatgatggc atggctcatt tccttgaact
     3841 tagccagatc attaaccact actgatccaa agaaagtctc accgtctttc agggtcggct
     3901 cgtaaatgat taccgttgct cccttggcct taatacgctt catgacaccc tgaatggagc
     3961 tctggcggaa gttgtcgctg ttgctcttca tggtcaaacg gtacacaccg atgatgactg
     4021 ccttttccct gtcttcatca aactcatttt ctgcatcata gccgtagtag ccagccagct
     4081 ttaggacacg atcagcgatg aaatctttac gagttctatt gctctcaaca attgcttcga
     4141 tcaggttctc cggcacatct gcatagttag ccagcagctg cttggtatcc ttgggcagac
     4201 agtagccgcc gtaaccgaag gacggattgt tgtagtggct gccgatgcga ggatcaaggc
     4261 agacaccatc gatgatctgc tgagtgttca gacccttgct ctctgcatag gtgtccagct
     4321 cattgaagta ggcaacgcgc agagccagat atgtattggc gaacaactta acagcctctg
     4381 cctcggtgaa gcccatgaac agggtgtcga tgttctcctt gatagcgcct tcctgcagca
     4441 gaccagcaaa ctcatgagca gcctcaacta aacgctcatc atttaagtca gtacccacga
     4501 taatacggct tggatacagg ttgtcataca gtgctttgga ttcgcgcaga aattctggac
     4561 tgaagatgat attcttgctg ttgaattttt ctcttgcact tgcggtgtag cctacaggaa
     4621 tcgtagattt aatgaccata atggcattag gattggactc cataactagc ttgatcacag
     4681 cctcaactgc agaggtatca aaatgctggg tttgggaatc ataattcgta ggtgcagcga
     4741 ttacaacaaa gtccgcatcg ctatatgcag cttttgcgtc tagagttgca gttaaattca
     4801 agtctttttc tgctaaatac ttttcgatat aatcatcctg gatcggagac ttacgattat
     4861 tgatcatctc tactttttct ggaacgatgt ccacagcagt gactttatga tgctgggaaa
     4921 gaagtgtggc aattgacaga ccaacataac cggttcctgc gacagcaatc ttgtagtttt
     4981 tcataatatt tactccttcg gtcctttagg acctcattct ccctaaaggt atatttttct
     5041 tgttgagtag acatttaatg gtgtgtacat caaataggaa gaaaatgata agaaaatgta
     5101 taaaatcatt ggagtctgtg tgaaagtaat aatgaagaag cgggtgagaa tacaggttgg
     5161 gatttatcag tagagaagac ggcatacagt gtctgagttt gttaacttgc tattttgcca
     5221 attagagagt aaaatcaata catacatgac gaaacccatt tattgtcctt ttacgaagta
     5281 aaaacggccg tttaggtaaa accacttgtt atggaaatgc gcatatgcta gtatgagaaa
     5341 accattattt ataaaggtaa aagggggatg ataaaatggg tgaagatgaa aataagaaaa
     5401 gggaacgaag gcatcaggag gatctggagc ggttaagagg ttttcggccg attgatgata
     5461 cgttcatgcg agggcttttt aaagagaata tacctcttgc agaactggtt cttcgtatta
     5521 ttacaggcaa accggattta gttttgctta aatgtgaaac acaggcggat atgaaacgtg
     5581 taaccggtgc cagatctgtt tgtctggatg cttatgcaac tgacagcaca ggcaaaaaat
     5641 atgatattga ggttcaaaga tcagacaatg gtgctgatcc acatcgtgcc aggtatcatt
     5701 ccagtatgct ggatatagaa tatcgtgata attcggatat tggcaggctg atgcatgatt
     5761 ttaattgtac caatgcatct gatatgtatt ttgatctatt ggcagagaaa actcgttatt
     5821 taaaagaaaa tccgaaggga gtgaacgaaa tgtgtaaggt tatggaagat ttaagaaatg
     5881 agagttatgc agagggacgt gaagaacagg ccaaatccac cgccatcaaa ctgaaccaaa
     5941 aaggccttcc catcgaagat atagcagatt ccattggttt cagcatcgaa actgttaaaa
     6001 actggctcac accaaaggca gtttaaattc ttcaaaacta ttatacgagg aagtaacagc
     6061 tatgaaaaac tacaggatca cagttgcagg aacgggatat gtggggctgt ccattgcggt
     6121 attgctttca cagcatcata aggtgacagc agtggatgtt gtgcctgaaa aggttgagat
     6181 gatcaatcat cgaaaaaccc cgatccagga tgagtatatt gaaaaatatc tggcagaaaa
     6241 ggagttagat ctgactgcca ctctggatgc cagagaagcc tacaaggatg cggattttgt
     6301 agtgatcgca acgcctacca attatgacag ccgcacacag cattttgata catcagcagt
     6361 ggaagctgtc ataaaactgg ttttggagta taatccagat gcgattatgg tgatcaaatc
     6421 taccattcct gtaggttata cagcttccat caggaagaag tttggcagca aaaacatcat
     6481 tttcagcccg gaatttttac gcgagtctaa agcactttac gataacctgt atccgtcacg
     6541 tatcattgtc ggtacagatt taaaagatcc acatctgaca gaagcggccc atatttttgc
     6601 gggtttgctt caggaaggag ccatcaaaga ggacatttcc accttattta tgggctttac
     6661 ggaagcagag gctgttaaac tttttgctaa tacctacctg gcattgcgtg tcagttactt
     6721 caatgagtta gatacctacg cagaaagcaa ggggttaaat acccgccaga tcattgatgg
     6781 tgtctgttta gacccccgta ttggcagtca ctacaacaac ccgtcttttg gttacggtgg
     6841 ctattgtctt cctaaggata caaaacaact gctggctaac tatgcagaca taccggaaaa
     6901 tctgatcgaa gcaatagtgg agagcaaccg gacccgcaag gattttattg cggaccgtgt
     6961 tctgaaactg gcaggctatt atgattatga tgaaaaattt gagttttcag cggataaaga
     7021 aaaagaggta acaataggtg tttatcgtct gaccatgaag agcaacagtg acaacttccg
     7081 ccagagttcg atccagggag ttatgaaacg gatcaaagca aaaggtgcga aagtagtgct
     7141 ctatgagccc gcattaaagg atggggaaac attttttgga agccgggttg tcaatgatct
     7201 tcagaaattc aaggatatga gccaggcgat cattgccaac cgctatgata agtgtctgga
     7261 tgatgtagca gaaaaagtat atacaagaga tattttccgc agagattaaa aggtttattg
     7321 cgtttgttta aagaagggag ttgataccgt atcagtaaat atgctgagat tatcctaacc
     7381 gatttcaaac agccggtctt ttatataata ccggctttct tgttgagcct tgtatttgtg
     7441 gtatcgttta catttacata tagggtttac aaaagggaaa aatacccact tcaaaactcc
     7501 gcaatatgga ccttgtttct ttgttactgg ctggttctga tccagactgc tttcttttcc
     7561 agggagtcgg gcagcaggaa gcagatcagt ctgatattgt ttgaaacctg gggacacagt
     7621 gtgcaggctc atgcttattt tatagagaac atttttatgt ttattccctt tggaattttg
     7681 gcgccggttt tatttaaacg aatgaagaag atgtactggt gtgtacttgc tggctttttg
     7741 atgtcctgta cgattgaaat atcccagctg cttacacaga gaggatatat gcagattgat
     7801 gatgtgctta ccaatacagc aggggcgatg gcgggatggc tgatctggaa gatcgtcacg
     7861 cttctgacag gctttttgaa gtccgaattc acttgaataa accctgactc tcggatatac
     7921 tagtacagaa agcgagaccg gaggttatta aaaaaatgga taatgattat ctgattaaaa
     7981 aaccgccgtt acaggcgtta tttttatttg cgcttcctat gataatagga aacctgtttc
     8041 agcagacgta tacaatggtg gactctgcca ttgtaggacg ttatgtcagt gagcaggccc
     8101 tggcagcagt aggagcgtct tatgctctta cgaatatttt tatctgcgtt gccatcggcg
     8161 gtggaattgg cgcgtcggtg atcgtaagcc ggtattttgg cgcgaaggaa tatggacgga
     8221 tgaagacggc tgtgtttaca gctcttcttt cttttcttgg catcagtctg gtcctgggaa
     8281 tagcaggact gatccttgga aagcagatca tgatatggtt gaataccccg gcagactgtc
     8341 tggatatggc gtcagagtat ctgcggatct atttcctggg gctgccgttt ttgtttatgt
     8401 ataatgtact ttctgctatg tttaatgcac tgggaaaatc caggatccct ctgtattttc
     8461 tgatcttttc ttccatattt aatgtgatcc ttgacattat catggtaacg aaaatggata
     8521 tgggagtggc cggtgttgcc tgggcaacgc tgatcgccca gggaatatct gcggttcttt
     8581 cctttggtgt tctccttaaa aatctgaagg gactggaagg aaagcagaag accttgtttg
     8641 acaaaatgga actggcggaa atgacgcgta ttgccatccc gtctattctt cagcagtcta
     8701 cggtttctat tggtatgatg ttagtacagt ctgtagttaa tggttttggc tctgaagccc
     8761 tggctggatt ttccgcagcc atgcgtattg agtcaatctg cgttgtacct atgtctgcta
     8821 ttggaaatgc tgtatcttct tttacggccc agaacattgg cgcagaaaga aaagaccgtg
     8881 ttgttatggg gcttcacgct gcaagcaaaa tggtcattgt ctgctccgta gtgatctgct
     8941 tcttcctgga agtgttcagc cattctatga tcatgctgtt ccttggaaat gaaggaaccc
     9001 agatcgcgct ttttacagga attggttatc ttacgtttat gggctggttt ttctgtttca
     9061 ttgggtttaa aatggcagtg gacggcctgc ttagaggtgc cggtgatatg aaaatgttca
     9121 cagttgcaaa cctggtaaac ctggggatcc gtgtgataat tgctgtcacc tgtgcgccaa
     9181 gatttggcat tgccatggtc tggtatgcag ttcctgtagg ctggtttgca aactggatta
     9241 tttcctatgc agagtatcgc acaggaaaat ggaaaacact gtagtgagtg caaaaacgga
     9301 actttaagaa actgttatac actatatgaa agaggtacaa tatggaacgt tacctgaaat
     9361 tcagagaaga atattcccgt ttcttttaca gaggttatga tgtagaagaa acattaaatg
     9421 aattaaagat cacatataca tttgaaatag aaggtctttc tgtttttaca cctacctgga
     9481 catttccaaa aaatggaaat ccagaagcaa aatggatgga agatcccctt atggaaaaaa
     9541 tgatcttctc cctgggaatg gtagagttgg tcagctactg gaagatcact tgctctccac
     9601 aggtggtgat cgaggcagga agtttatcag aagagcagat cctctggtgg aaagatctgt
     9661 attttaatgg tcttggcgaa tttttctatg taaataagat cacagaggca gatccggaag
     9721 gatttatgga gatcgtctgc ccattaagtg aagaagagtc tgcggcaaac agttacgaaa
     9781 agaagcagga cacagcaggc gccgaacagg gcgttcttgt tcctgtagga ggcggaaaag
     9841 actccgcagt taccttagag ttattaaaag gtgcagatgt accggtattt gcctacatta
     9901 tcaatccaag aggggcaacc atccatacca cagagtctgc gaaactgaca gcagaccatg
     9961 tgatcagtgt acaccgtaca ctggataaga gaatgcttca gttaaataag gaaggtttct
    10021 taaatggtca tactccattt tcggcgttag ttgcattttc cagtgtgatc gctgcaagaa
    10081 tggcaggtct ttcttatatt gccctgtcaa atgagtccag cgccaatgag agtacggttc
    10141 agggaagtac tgtaaaccat cagtattcca agagttttaa gtttgaggaa gatttccatc
    10201 agtatcagac cgatcatctt ccaggcagcg cctattattt cagcatgctg cgtcctttaa
    10261 gtgagttcca gatcgcaaaa tattttgcaa agcagaagca gtaccataag atattcagaa
    10321 gctgcaacgc aggaagcaag acagacagct ggtgcggcca ttgtccgaaa tgcctttttg
    10381 tatatctgat cctttctcca ttcctttcca gagaggaagt aaaggagatt ttcggaacag
    10441 atatgttaaa tgatccggat atgcagccaa ccttagaaca gttagtagga attcaggagg
    10501 aaaagccttt tgaatgtgtg ggaagcaggg atgagatcaa cacagctatt gttatgacta
    10561 tagataatct ggaaagatct ggaaaagagc ttccttatct tttaaaatat tacaagacca
    10621 caggcctgta tgaagcttac aaggcaaagg gagatcagtt ctcttcttat tttgataaac
    10681 aaaatttggt tccgactcca tgggagaagc tggtagagaa aaactgtaaa gatgaggcat
    10741 aaaactatga tgatcgaaag aataagaccc tgggtccagg ggaaaaaggt tctgatcctt
    10801 ggatttggca gagagggaaa atctacctgg aatgtactga aaaagctgga ttgctgctct
    10861 ttcgtagatg tggcagatat ggcagaaccg aaggaaaagc cggaggggat cagaaactgg
    10921 atcgccggac cggattatca gaaatgttta aatgaatatg atgtggtatt taaaagtcct
    10981 ggcgttgtat tagaaaagcc ggttcagtct tattccagtc agatcctgtc ccagacagaa
    11041 ctgtttttcc agtgtttcag ggatcagatc atcggaatta caggcgtgcc ttgatagatt
    11101 taaaacaggt atgggggata aatataagga tcatattttt tcagaggaag aaattcaaga
    11161 aatcattcgg ggcttttttg agcagaatag agagctgcgt annnnnnnnn nccatttgca
    11221 aagggaaaaa gtacgatcac aaccctgatc tatcatctgt taaaggaagc tgggatggat
    11281 acgatcctgg taggaaatat cggtattcct gtgtttgatc atatggaaga agtgggagag
    11341 gacacaaaga tcgtatgtga actttcctgt caccagctgg aatatatgac tgtttctccg
    11401 catattggaa tccttaccaa tctccatgag gaacatctgg atcactatgg aacactggag
    11461 aaatatatac aggcaaaacg ccatatttat ttaaaccaga aggaagaaga tgtgatgatc
    11521 tgcaatgtac agtgtctgcc tgaaccgggt acctgcaagt cgaaactgat cgcagcaaag
    11581 ccttcttttg aagaaccggt atttcagatt ttgggagtac ctggtcaggg agctgtttca
    11641 gaagaaatgg tcaacatctg gaaaaaaatg gaagttatac aggaaaatga tactacagag
    11701 gccagatatg acggccattc cattgaaatt cctgtatccc agatccgcct tttaggtcag
    11761 cataattatt ttgatattgg aatcgcctgg gcagtatgcc gccttttaaa tatcagggac
    11821 gaggtgttct tagaaggact taaaacttat gaaccgctgc cacaccgcct gcagtttttg
    11881 ggagaaaaga atgggattaa atactatgat gactccattt ctaccatttg tgagacaacg
    11941 atccaggctt taaatacttt aaaagatgtg gacgccgttc ttattggtgg tatggaccgg
    12001 gggatcgact acagtgagct gatccgtttt ctgtccggtc atccggtacc gtatattata
    12061 ttaatggaag caacaggaaa acgcattttt gaggagatcc ggcaggatta tccggatttc
    12121 cagaaaatgg aacgtctgat cttaactgat cacttagaag atgcagtaaa agaagcgcaa
    12181 aaacttacaa gaccgggcca cagctgttta atgtctccgg cagctgccag ctatggtatc
    12241 ttcaagaatt ttgaagaacg tggggaagtt tttgcccgtc tggtattaaa ataagccgga
    12301 aaacatagaa aaatcacatt tttttaagaa aatataagga ttttaggact tgtgtaccag
    12361 aataaaataa gatacaatct gtaagaagct tttggaaacg tgaaagggaa taatgtggga
    12421 acctacgata cgctgaatga tgtacttgta aatctgtttc gcgatatcaa cagtatagaa
    12481 gaaaatgcga taaccagtca ggaatataaa gatctgacca ataacgatat gcatgtgatc
    12541 aatgccatcg gggtcgggac aacgaaaaat atgtcatcta ttgcaaagga attgtcagtg
    12601 acggttggta cactgacaat ttccatgaac agccttgtga aaaaaggtta tgtgactcgc
    12661 tgccgcagta cgcaggatcg ccgggtggtt catatttctc tttcggaaaa aggtgtcaaa
    12721 gcttattatc atcacaaaca tttccatgaa cagatgatac agggagtggt tgaggtgttg
    12781 acggaagaag aattgcaggc tttgatcaag gcactgacaa aactgaattc ctggttcaga
    12841 agttttggca aggatgaata aggaggcaac atcagaatga aaaaatggtt attaggttta
    12901 cttacagcta tgatggcagt ttcactggct gcatgtacac caacaactga aaagaaacag
    12961 gaaacaacta caactgcagc tgcagcgaag caggagcaga ttgaagcaca gactaccggc
    13021 ggagccagcg ataaggtacc ggatccaaat gtagcacctg tagcgatcat ttccgtatat
    13081 cacaagggtg atggtgacag cctggttcag gatatggatt ctttggacaa tgatgaactg
    13141 gatccacagg ctctggtaag taagatgacc gagtatggta tttttacaga aggcacacag
    13201 gttttaaatt ttaacatcac cggagaaggc gatgttaaga aggcagtatt agacctgaac
    13261 aaagtggaaa atgatgaagg cgtttctgac agcgtatttc tggtagaaat tggaaatacc
    13321 tttattgaaa actttgagtt atccagcctg actctcaagg aaaatggagc agacctggca
    13381 ggagctacag atcttactta tgtagctgat tatgagaatg tggattaata atgattataa
    13441 atgatcgtaa taatggatca tataaagaca ccctgaaaga caagatctgg tttttcaggg
    13501 tgtttttgtg tttacagaca gacatacttt tatacacaca ctgcatacaa taagtactga
    13561 cgtagtaagt cagaactggc aaaaatgggc tgtgggaagt gaggagtgga agtatggctg
    13621 gttatcgcag gttgatatcg tatatttatg catatgaagg agaagaaaaa gggaaaaaca
    13681 ttggtttcgc gaagctggaa gccaggaacg gacagtgccg tctgagcata aatgtaaaga
    13741 agatctatgc agggggcaat gatatgggag tatatttgct gtcttctggt caggagatct
    13801 ttttaggaaa tatttttatc cgcaatggca gcggggattt caggacctgc gttaatgtgg
    13861 aaaatgtaat gggaacaggc tgttctatgg acagctgtat cggtctggtg atccatgaaa
    13921 agggagagga ctggaggatc tacaaaactg tctgggagga tgcagtagcc cagactgcag
    13981 aactggaatt tgcagaggtt acttctgaaa agaggggaga tctcgatgag cttttgaaaa
    14041 agcacattga agagctgaac caggagatcg accaggcggc agaggaagca gccagggagc
    14101 ttgaaaagca gacattggaa caggaagcag aagtgcagga aacggaggct caggaagcag
    14161 gggctcaaga agcagaaacg caggacgttg agacacggga aacaatagaa gctcctatac
    14221 catctgcacc agccccacaa ccccagacca ggtccaaaac atctcaacca gaagagatcc
    14281 ccttaggcaa cccagaagaa ttagaacgct tagatcagga agaagaaaga tgggggccgg
    14341 acgagctgtg gaacaggttt cggaaacatt atcctaaaat gcaggccttt gacagtccgg
    14401 gaactgtaga gattttgtcc atccggccgc aggatatcgg ccttcttcca agggagaact
    14461 ggggatacgg aaataacagt tttcttcttc atggttatta caattacaga tatcttattt
    14521 ttgcccgtgt gggagatgaa aaacatgcca gatccaggta catattaggg gttccgggac
    14581 attattacag caatgaaaaa tatatggcat ccatgttcgg ctttcctcat tttgtgctgg
    14641 caaagaccca gccggcccag gacggccgtt ttggctactg gtatgcagat gtaagaatgg
    14701 aaaatgtaga gtaggcgttt ctcatttttc agtgtatact aaaacctgtg gaataataaa
    14761 tatacagctc tgataccgaa ttgctacgtg ctttgcactt tcgcaattct aaaaacatga
    14821 aagggataaa agatctatgg atgaagcagc acagaaaggt cttgtcacca gacagaggcg
    14881 ctgggaagcg agaaatgaaa aacgccgtca gaaagcgttg gaagaggaag cgaaagctgc
    14941 aagaataaag gcagaagacg aaaaatacat gaaggaagcc attaaacagg ccaaaaaagc
    15001 agccaaaata ggagatgttc ccattggctg tgttcttgta aaagatgggg agatcattgc
    15061 aagaggttat aaccggagaa atgcggataa aacagtcctc tcccatgcag aggtcacttc
    15121 tataaaaaga gcctgcaaaa aagaaggaga ctggcgcttg gaagactgca ctctttatgt
    15181 gactctggag ccatgcccta tgtgtgcagg tgccattgta caggcaagga tccccagagt
    15241 ggtcatcgga tctatgaaca gcaaggcggg ctgtgccggt tctgtcctca atttattaaa
    15301 agagcctggc tttaatcacc aggctcaggt cgttacaggt atattagaag aagaatgttc
    15361 tgaaatgatg tcaagctttt ttactgagct gcgaaagaag aagcgtcttc cggaaactcc
    15421 ttaaaaatac cccggatcac aaacagggac aatataaaag tggcaatgtc tgccagtggc
    15481 tgggcacact ggatacctaa aagtcctatg gtataagtca tgatcgtaag cagcgggatc
    15541 aggaaaattc cctgacgggc tgtagatacc aaagtagccc tgaaaccgta tccaatagac
    15601 tgaggcatca tatttcccat gatcacagct ccctggagag gcagggtgat caactgcagt
    15661 ctgagagcca gcgttccgat ttcaataaca gtcgggtcat tgcgtcggaa tagtgtgata
    15721 atagagcggg ctgccgggaa actgatcacg ctgagtacca gaaggataat ggtgcttaca
    15781 cggatgcaga accagcaggc tttcttcacg cggtcgtaaa gtccggcgcc gaagcagtag
    15841 ctgcataccg gctgaaagcc ctggccaaag ccgataatga cagagttgat gaaaaacata
    15901 aaacggctga caatggacat ggccgcaatc gcagcgtcac cataaggctg acacataaag
    15961 ttcatcagga tcgtagccac actggcaatt ccctgacgtc ctaaagatgg gatgcctcca
    16021 tagaggacct cgcgatagat cgccagtttc gggcggaatt tgcggatgct gatggaaatg
    16081 cagctgtcct gatgattgca catccataaa aggatacaga aactgataaa ctgggaaagt
    16141 ccggtagcta aagcagcacc tgcagttccc atgtccagag taaagataaa cagcggatcc
    16201 agtgcaatat ttaaaagacc gccgactgta atgccaacca tggcaaaaga agcttttccc
    16261 tggaaacgca gcaggttgtt cataataaaa gagcagatca taaagggtgc tgcaatgaaa
    16321 atatagtggg cataggcttc tgcataaggt gcaatggttt ctgttgcgcc taaaagaaga
    16381 accacccggt ggatgttaaa ggtggcagaa acagtgatca ccagtcccat gatgatacct
    16441 gtaaaaaagg caatagaaac gatctgttct gccagttctc tttttccggc accaagggaa
    16501 cgggtcatat agttgccgct tcccattccg atcataaatg ccagtgcctg tataatggac
    16561 atggcagaaa agataacgcc tacagcaccg gaggcgctgg ttcccagctg acttacaaaa
    16621 aatgtgtcag ccatgttgta aatagagctg atcagcatac tgacaatagt aggcgccgca
    16681 agctttggaa tgatatggct gatgggggcg tttgccattt cgtaatactg atcatgcttt
    16741 ttttcagtat gactcatata aatttctcct taattttttt gttcgttctg aaatttatta
    16801 taacagagga aaaaatgaag tacaagtgag ccttgacaaa tgctggtctc acaggtatac
    16861 tggataagca taaatttgtc ataaagtttc cacgcagccg gggagatagc ggtgccctgt
    16921 atctgcaatc cgctccagca gaggtgatgc cttgcctcgg atcggtcatt gcggagctgc
    16981 cttatgtaag tggcgttgac gtttgggtct tacgcaacgg gaacctgtga accgtgtcag
    17041 ggcaggaatg cagcagcact aagcaggaac tctcgtgtgc cgtaaggcag cctggaccga
    17101 gttaactgca tgagtaacgt ccgtgatgcc ggtacaaagc aaggcgcgtg gattaaatat
    17161 ataattgata acgaatgacc cgtctgcgtc agcagacagt ggagcgcgat agcgtggggg
    17221 tcatgagtga ctacaagcac aaaaataagc aagcctgagg actgtcataa aagaaatatg
    17281 gcagatttcc ccaggctttt ttgtgtcagg acacattctt tttaacttgc cccttgaaaa
    17341 tctttgcgtt ataattgcaa aataggtata gtagtatcat tcttatgggc aacaacgaaa
    17401 aatcctgatt tctgattatg gaggaaatgc aatatgagaa gaattggtat cttaacaagt
    17461 ggtggtgact gccaggcttt aaacgctaca atgcgaggcg tggcaaaatc tttgtacaat
    17521 atgttagatg atgtggagat catcggattt gaagacggat ataaaggtct tatgtatgca
    17581 gattatcaga ttatgaaacc agcggatttt tccggtatcc ttactcaggg cggaaccatt
    17641 cttggaacat cccgtcagcc atttaagcag atgcgggtcc cggatgaaaa tggccttgat
    17701 aaggtagaag caatgaaaca tacctataaa aaactgaaat taagctgcct ggtagtcttg
    17761 ggcggaaatg gaagccagaa aactgccaat atgctcagtg aagaaggctt aaatgtagta
    17821 tcccttccaa agaccattga taatgacctc tggggaaccg atatgacctt cggatttcag
    17881 agtgcagtgg aagtggctac caatgccatt gactgtatcc atacaacagc agcttcccac
    17941 ggaagagtct ttattgtaga agttatggga cataaagtag gttggcttac tttgtatgca
    18001 ggtcttgcag gcggagcaga tatcattctt cttccggaag ttccatatga tatccaggta
    18061 gtatgtgatg cgatcacaaa gcgtaccaaa gcaggaaagc gtttttccat tcttgcagtg
    18121 gcagagggag ctatttccaa agaagacgcc gctatgtcca agaaagaatt aaaagaaaag
    18181 aagaaaaacg gcgtagttta tccatctgta gcttatgaga tcggcgccaa gatccaggaa
    18241 gtgacaggaa gtgaagtacg tgtcactgtt ccgggccata tgcaaagagg cggggaacca
    18301 tgcccttatg accgtgtgct ggcaaccaga ctgggagcag ctgcggcaga gcttattata
    18361 aacgaagaat atggctatat ggcagctttg aaaaataatg agatcactaa agtaccatta
    18421 tccgaagtag ctggaaaatt aaaaacagta gatcctaact gttctatgat caaagaagca
    18481 aaacggatcg gtatcagctt tggtgaccag taaaaaataa ggagtaagaa aatgtcgtat
    18541 acggcattat atagaaaatg gcgtcctgtc tcttttgaag atgtaaaggg acaggaccct
    18601 attgtccaga ccttaaaaaa ccagatcaca tctgaacgta ttggtcatgc ctatctgttc
    18661 tgcggaacca gaggtacagg taagaccagt atagcgaaga tctatgccag ggctgtaaac
    18721 tgcgaacatc cggtagatgg aagcccctgt aaccaatgtc cttcctgcaa gtccattctt
    18781 gcaggaactt ctatgaatgt agtagagatc gatgcagcat ccaacaatgg tgtggaaaac
    18841 atccgtgaca tcagggaaca ggtacagtac ccgcctacag agggaaaata cagggtttac
    18901 atcatcgatg aggtccatat gttatccatc ggtgcattta atgccctttt aaagactctg
    18961 gaagagccgc cgtcctatgt aatatttatt cttgcgacca cagaagtcca taagatccct
    19021 attaccattc tgtcccggtg ccagagatat gattttaaac gtatttccct ggagactata
    19081 gctggcagac tgcgtcagct gactgaggaa gagcatatag aggtggaaga taaggctctt
    19141 atgtatgttg ccaaggcagc agatggctcc cttcgagatg cgttaagcct tttggaccag
    19201 tgtgtggcat ttcactatgg aaaactgctg acttatgaaa atgtactgga cgttctcgga
    19261 gcagtagact ccggtgtatt tagcagtatg tttaatgcag tgatcgaagg acgtaccaaa
    19321 gactgtatta cccagttaga ggagatcgtg atccagggcc gtgaattagg tcagtttgta
    19381 acggatttta tctggtatct gagaaatctg ctgctggtac agagcgcaga tgatgcagaa
    19441 ggtcttctgg atatgtcaga agaaaactta aaacagttaa aagaagactc tacaaagaca
    19501 gatggaaata cgctgatgcg ttatatccgt attttctcag aactttccaa tcagctgcgc
    19561 tatgccagcc agaagcgggt tatggtggaa gtggctttga tcaaacttac cagaccgtct
    19621 atggaaccgg acctggactc tgttctccaa cgtttaaaac agttagaagc agctgtagaa
    19681 gatctggaag caggaggttt cagacctggg accatggtca cggctcccat gggggcagtc
    19741 cctgtaactg caggtgtaca gcctcagata tcaggaacac agggacagat ggcagttcca
    19801 gtccaggggg gtcctacagg aaatccgcct gaaacagctg gcgcagccgg tattacgcct
    19861 ccaaatcagg tcagccttcc accggcccag ttagaagatc tgaacctgat tcgcaatgaa
    19921 tgggcaaaga tcatacgaag tgctggaggc agcgcaaggg catgtttcag ggatacagtg
    19981 gtagaacctg gcggagaagg ctgtcttacc attgtatttc ttgactctat gagctatgat
    20041 atgggaaggc gtcctacggt gatcggccag ttagaacagc tggtccaggc taattatgga
    20101 aaatctattt attttaagac ccggttagcc ggtcgcggag agcgcctgga cacaatatac
    20161 gtgacaaaag aagatctgga agataagatc catatggata ttacttacga agattaaaac
    20221 gaatttattg aatgaagaga tgatgaggag gaacacacca tggcaaaacg tggcggattc
    20281 ccaggcggta tgccgggaaa tatgaacaat cttatgaagc aggcacagcg tatgcagcgc
    20341 cagatggaag agacaacaaa agaactggaa gaaaaagaat acacagcagc tgctggcggc
    20401 ggcgctgtta cagtaactgt ttccggtaaa aaggaagtag tttctattaa gttatctgaa
    20461 gaagtagtag atcctgatga tattgaaatg ttacaggatc tgatcgttgc agctaccaat
    20521 gaagcattcc gtcagatgga agctgcaaac agcgaagcta tgtccaagtt aacaggtgga
    20581 ctgggcggtg ctttaggcgg aggctttccg ttctaatgga ctactatagc agtcagatca
    20641 ccaagctgat cgaagagtta tcaaagcttc cgggagtggg ggcaaagtct gctcagcgcc
    20701 ttgcatttca cattatcaat atgccgaaag aacaggtgga tgagctggct ggtgctatga
    20761 caggcgcccg gaataatgtg cgttactgca aggagtgttt tacactgaca gataaagaga
    20821 tctgtccgat ctgcagcagt gacaaacgaa accataaggt cattatggta gtggaaaata
    20881 caagagatct ggcagcttat gagaagaccg ggaagtacaa tggggtgtac catgtgcttc
    20941 acggtgccat ttctccgatg ttggggatcg gacccaatga tatcaaatta aaagaactga
    21001 tgcaccgtct tcagtctgat gtggaagaag tgatcattgc cacaaattcc agcctggaag
    21061 gtgaaactac agctatgtat atcagcaaac tgatcaaacc tacaggcatc aaagtgacca
    21121 ggatcgccag cggcgtgcca gttggcggag atctggaata tatagatgag gtgactcttt
    21181 taagggcact ggaaggaaga acagagctgt agaagctgga agaaaaggca cagggagatg
    21241 cataagatgg ataaatatga gtttaatatt aaagttgagc agataaaaaa gctggtcaat
    21301 aaaagtgatt atgagacagc catgaagatt gcggatacca ttgactggag aagggtccgt
    21361 aatgtaaata tcctgtctat ggtcgcaggc atttatgaga aaaacggaga gttccaggaa
    21421 gcaaaagata ttctgcttct ggcgtttgag agagctccta ttggcaaacg cctgctttat
    21481 aagctggcag atctggcttt aaaagaaggc aatgttgagg aagcagaaga ttattacaaa
    21541 gaattctgca acctggcagc agatgatcca aggcagtata ttttaagata tatgatctta
    21601 aaggcaaaag gtgcgcctgt agaacagctg atccataccc tggagcagta ctgcaacgca
    21661 gaactggatg aaaagtggat gtatgagctg gctgaactgt acaaccaggc aggaatggag
    21721 cagctttgcg tcatgacctg cgataagatc atgcttatgt ttggtcttgg aaaatatgta
    21781 gataaagcca tggagttaaa gcttcagtat gcacctttaa accagtatca gatggatctg
    21841 gtagaaaata aagagaagta tgaagcaaaa ttaaaggcag tagagcagga atacaaaaaa
    21901 ggtacttcta ttgtacagcc gattgaaaat acagaccgcc gtcagttaga cagggaagaa
    21961 gagccggtgg aagatacagc agaagatgag actgtttatg ctgaggatga atacaccgaa
    22021 gattaccggg aagaaccggc tgttaccatt caggatgatg atataacaga ggaagaaccg
    22081 gtttctgagg agcctgaaga agaactggca gaagagcctg aggagactta cgagacagaa
    22141 gaacctgaaa cagaagatta tgaggcaggg gagtctgaag cggaagagtc tgaagaggag
    22201 ccggaagcgg atgaagagca ggctgtatat gaaacagcag cagcggatga agaaatccgt
    22261 gccagcatcc atgaagcccg tgcgcaggaa aatctggcaa gagaaatgtc aaaattatct
    22321 gcagcagcag aggaagaaga tctgccggaa gatgaaatgg gacagaccag agcgcttaca
    22381 gatatccgtg agctgagaaa aggtcctgtt tatacaggtt ccaaccattt gatgatcgag
    22441 gcagaggttc ctgaaaaggg acttgcgctg gcagtggagg cactgcgtaa aatacataaa
    22501 gaaacaggcg caaagaacca ggcagcaaag atctccggcg aaaaattaaa ccgcagaggt
    22561 gtatttgcgc tggcagataa gctgacagga aaagacctga tcattgagca ggccggtgat
    22621 atgaatgaaa gcatccttca ggaactgaac cagttgatgg caagggatga gacgggtatg
    22681 aatgtggtcc tgatcgatac agcaaggcgc ctggaagaac tgcaccgtgt atatccggga
    22741 ctggcaaagc gctttgagtg cattggaact tctgacagca taaaagcgaa gaaagcagca
    22801 gctcctgtac catctgagtc tggaaaagga tctctggcaa aggctcctga agttgttgag
    22861 aagaaagtac agccagctcc aaagccagtg gcagcaccaa aagaggctcc ggctccaaaa
    22921 acggcggcaa cgaaggcagt tgtaacttcg aaggcagctg cgccaaagcc tgcagccaaa
    22981 ccagcagcac agccggaaaa gaaggcagct gcaccagcta aaaagcgtcc ggttcaggca
    23041 ccggctgtaa aagaaccagc tcgtatggca gagacagaac agccagagca cagcgagcag
    23101 gaaatggaca ttgatgagtt cgcaaaatat gcctgccagt atgcgagcaa gatcgactgc
    23161 agtatttccg gaaagagtat gttagctctt tacgagcgca ttgagatcat ggaagaagat
    23221 ggcattgccc tgaccagaga agaagctgaa aaccttattg aagaggcagc tgacagagca
    23281 gaaaatccat ccttctttaa gagacttaca ggtattttct cttctaaata cgataaggat
    23341 ggcctcctta tactgaagga ggagcatttc ttatgagaaa tataaaatta atagctctgg
    23401 acttggacgg aacccttctt aacatggaaa agaaggttcc tcagggaaat taccaggctt
    23461 taaaagaatg tgaaaaagca ggaatccaga tcgtgccggc aaccggaaga ggcgtaggcg
    23521 gtattccgcc tatgatccgg gaacttccag gggcaaacta cgccatcacc accaatggag
    23581 ctgttgttgc agatcttaaa aataataaag cgatcaagac ctgtggactt tctaacgaaa
    23641 tgatacagcg tatcttaaat attgctaaaa agtatcactc tgccacagat ccttttatag
    23701 atggaagagc tattacagag ccggcatcca ttgatcatat ggatgaattt gggctcagcc
    23761 cggaaatgca gaaactgatc cgggatacca gggaagttgt tccaaatgta atggaatatg
    23821 taaagacaac aggagcagag gcagaaaagg tcaatatttt tatggcggac ttagaggaaa
    23881 gagaagttct tcgtaaggag ttaatggcga tccctgagct gtctatcagc tcttctatgt
    23941 acaataatct ggaggttaat gcaaagggcg ctgacaaggg ctccgctctt ttatggcttg
    24001 cagactatct tggcattgac cgggaagaaa ccatgtcatt tggagacggg gaaaatgata
    24061 tccctatgat ccaggcagcc ggaattggcg tagcaatgga aaatgccctg gatacagtaa
    24121 aggcagcagc agatatgatc actcttaaaa atgatgaaga tggcgtagca gctgcaatca
    24181 gaaaaatcat atttggatca aatgaaaaat gatgacggaa cattggctat caatcgcagc
    24241 aggtgtgttt ttagtgggaa tgatgttata tggtcattac cgcggcctgc tgcgtcagtg
    24301 tgtttctctt ggagcgctgg tactgacgat cattaccgtg aaagtagcaa caccgtatgt
    24361 cacagatttt gtaaaggcaa atcctgtgat ccgggaaaat gcggctcatt ttattatgga
    24421 agtatccgga tggcagccgc cggaggagag cgaaagcatt cttccggcgg cccagcgtat
    24481 ggcgatcgaa gaactgaaac tcccacaatc ggtaaaagat gttcttttgg aaaataataa
    24541 cagtgaattt tacaggcttt tgggagtaga tcagtttgga gagtatatca gtacctgcct
    24601 ttcagatatg ctgatcaata cccttggttc tgttctggta tttgcagcca gttatatagt
    24661 gatccatttg gtgatccgct ggctgaacct gttgtccaga ctgcctatta tatgtggctt
    24721 aaatcagatg gcaggaggga tcgtagggct tgcagaagga tttctgcttt tgtggcttgc
    24781 aggatttatt ctcagctttt ttacggagac tccaatgggg cagatgctgg aagcgcaggt
    24841 aaacagcagt atctggcttt cactgatcta cagatataat cttatgaatg tgattctggg
    24901 tagtatcatc aatggtattt tatagtacaa acatactgaa aatgaaatag atttaattta
    24961 gcatataaaa tatgcaataa caacaaatca ctgggaagtg catttattca gtgcagatcc
    25021 ggtgatttgt tttatatttc aatctgttat caggacctga aaaacaagga aaatcctgga
    25081 aaaacaggta atgtatagag cgggaaaaaa ttgtgtaaaa aaatactaca aatcctcttg
    25141 acaatagctg acggagatgt tatattatag ttccattgcg aaagcagtgg ctcaaataaa
    25201 aaagaatttg agaaaaataa aaaagttgtt gacaaaagca atgatacatg ataagatgat
    25261 tgagttactg atgagcaaca aaaaataaaa taaaaaaagt tgttgacaaa cttaaacaac
    25321 tgtgatagaa taatcgagct gttgaaaatg agacaacaac aaagaacctt gataattgaa
    25381 gattaaacag tatgtaaaac cctgaaaatt ctaaaataa
//
LOCUS       DBMDUA010000016        25083 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1349_length_25083_cov_3.740974, whole genome shotgun sequence.
ACCESSION   DBMDUA010000016 DBMDUA010000000
VERSION     DBMDUA010000016.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 25083)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25083)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25083
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1349_length_25083_cov_3.740974"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="ACZPCL_02515"
     CDS             complement(<1..1221)
                     /locus_tag="ACZPCL_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIR1161392.1"
                     /translation="MKEVSFQESKFVEKFTIFASKMGNEIHLRSLRDAFATIMPLYIL
                     AGLAVLINNTVFVWLLSGDTLTGAQYWGTVITNGTLNISSLLIAPVIGYCLSRNKGFD
                     NPMAAAIVSLAALVVMMPNVVSVTPLEGGESVAVSGALTFGNVGTQAMFAGVIIGLIA
                     TELFVWISGIKKLQIDLGDSIPPSVGKSFNVLIPFILLLSGFGLISAVLNNVFDTNLI
                     QMITTLIQEPLRTVTTGLVGCVILYSLGNFLWLLGIHQSVIYSSILEPLLIVNMTQNM
                     AAYAAGEAIPNIINVALVPGFGMMGGSGSTICLLIATFLVGRNKATRGVAKMAFVPGL
                     FNINEPVIFGYPIVYNISLIIPFILTPALGIIIAYAATAMHFMNECVVYIPWTTPPLL
                     SGYLATAGDWRAILV"
     gene            complement(1218..2618)
                     /locus_tag="ACZPCL_02520"
     CDS             complement(1218..2618)
                     /locus_tag="ACZPCL_02520"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIR1161393.1"
                     /translation="MELKIPENFFVGAAMSGPQTEGAYKTGGRLESIWDTWSDLSISD
                     FYNKVGSYVGNNFYEKYEDDIKLLKSLHMDSFRTSMQWSRLLTKDGKLNQEGAQFYHK
                     VCKCAKENQIELFMNLYHFDMPTYLFERGGWESRDVVEAYAAYARAAFREFGSEIRYW
                     FTFNEPIVEPDQRYRNGFWYPFIKDAKRGMQVQYHLSLAHSLAVWEFRKAKEEGYVRE
                     DAKIGLINCFAPPYTREDPTPEDLEALRMEDGINNRWWLDLVAEGHLPEDVLSTLEEK
                     GLAPERRPGDEEILKQGKVDWLGFNYYHPSRIQAPKEKTDENGYPKFSDPYVWPEAKM
                     NIYRGWEIYPKGIYDFGMKMKNEYPDLKFFVSENGMGVEHEDRFRDASGEIQDDYRIE
                     FITEHLQWIFKSIEDGAKCLGYHYWGVIDNWSWNNAFKNRYGMIEVDLMGNYSRKLKK
                     SARWMKGLLEEREAKV"
     gene            complement(2856..3575)
                     /locus_tag="ACZPCL_02525"
     CDS             complement(2856..3575)
                     /locus_tag="ACZPCL_02525"
                     /inference="COORDINATES: protein motif:HMM:NF019322.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1161394.1"
                     /translation="MYKYEMIAEEIQKKIKDGTYPPDTRLPQEMEMCKIYDASRITIR
                     EAMELLVAKGLITKRRGAGTFVKAVAPGMTDQVGFSKSGQFSGFSKDQAGRKVSSDVH
                     EFGLKKASKEVAEKLQVAENAFVCYICRTRLVDGKPYVVEYTYMPTDFITGITEDVVK
                     SSIYSYLENDLGLKIQSAHRIARADMPTEQEKKWLDIGDQVLPVLEVEQVAYLDDGRI
                     FEYSISRHRADRFELKSVSIH"
     gene            complement(3678..4319)
                     /locus_tag="ACZPCL_02530"
     CDS             complement(3678..4319)
                     /locus_tag="ACZPCL_02530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1161395.1"
                     /translation="MNIMIFADQESKLLYEYYDPEQMKDIDLIISCGDLEPEYLTFFA
                     TLCHAPLLYVKGNHDTKYEYKPPEGCICIDDDIFVYKGVRILGLGGSMEYIPDSPNQY
                     TEKMMRKRIHKLWWKLWRHKGFDILVTHAPAYKLNDMEDLPHRGFKAFLDLMDKYHPK
                     YFLHGHVHANYGSGFKRKDVYGTTRVINGYEFYVIEYPGEPVVPAEKTEDTNA"
     gene            complement(4320..6272)
                     /locus_tag="ACZPCL_02535"
     CDS             complement(4320..6272)
                     /locus_tag="ACZPCL_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1161396.1"
                     /translation="MSDTEYGAALKLGKKQYQDAVAKGEYPYLPVLDDVLSYTDIVST
                     VSLGTMDIPLAKLVGTKTAERSNSFANNFMPLLPERSEFGIKWLNLYNHQVEDGIQDP
                     IVAYEFMNRFYVQEGNKRVSVMKYLGAYSIPGTVTRLIPKRTDDLENRLYYEFLDFYK
                     VSSNCDVWFSKEGRYKELLKLMGMKPDQVWEEEDRVYFRSAYGRFAKAFSMAHGDRLK
                     LTEGDAFLVYIEVYGYDNVKGQTEREMYRALMRIWEEIQLANRGNKIELIQDPQEVEE
                     DKKPAILKWFLPQEEIPSGLKVGFIYGRTAETSRWIYGHELGRMYLEQAFPGKLTTMV
                     VDQADTEEAVAEAIEKEAKAGCTIIFTITSRMLGQSVRSAALYPDIKFYNCSINMSYS
                     SVSNYYARMYEAKFLMGAIAAALSREDRLGYIAEQPTYGMLADINAFALGARMVNPYV
                     EVHLEWARRKEAQHTEDILHEKGIHYISGHDMINPDHPSREYGLYRKNEDGTVTNLAM
                     PVWHWGKFYEQIIRLAFKSTEEIEAMKGKKAVNYWWGMSADVIDVICSENMPNGTRRL
                     IEFLKNSIRAGSFHPFDGLIYAQDGSAKCTQGKSLNAEEIITMNWLAQNVVGYIPKIG
                     EMNERAQELMQLQGIKATEQTEMENE"
     gene            6454..6714
                     /locus_tag="ACZPCL_02540"
     CDS             6454..6714
                     /locus_tag="ACZPCL_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0065007 - biological regulation [Evidence
                     IEA];
                     GO_process: GO:0065009 - regulation of molecular function
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM1266 family iron-only hydrogenase system
                     putative regulator"
                     /protein_id="HIR1161397.1"
                     /translation="MEDRLAVISCIISDPQSVSVMNEKLHLHASMIIARLGVPCREYG
                     VSVVSVVLHGTQNEISSFAGDLGKVKGVKVKSIQVPVNSTSD"
     gene            complement(6730..10260)
                     /gene="mfd"
                     /locus_tag="ACZPCL_02545"
     CDS             complement(6730..10260)
                     /gene="mfd"
                     /locus_tag="ACZPCL_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR1161398.1"
                     /translation="MENPLLELQEYENLQEALKKAKGPVQATGTLDSQKVHLMYWLGK
                     DGGFPWKLVVTYDDQRAREIYDDFRSFTENVWLYPAKDLLFYSADIHGNLMTRQRIAV
                     WRHLMEEKEGVVVTTLDGLMDHLLPLVFLKQQAVTVENGQIMDLDEWKERLTALGYER
                     TAQVDGMGQFSIRGGIVDIFPLTEEVPVRIELWDDEVDSIRSFDPESQRSVEQLEKIT
                     IYPSAETILTKKQLEAGIERLEKEEKEYEAALRAKHQIEEASRIRGIIKELTEGIRQG
                     WKRDGLDAYIRYFCPETVSFLDYFPAGDTLICLDEPARLKEKGETVEQEFRESMIHRL
                     EKGYLLPGQTELLYAGTQVLAKLQSSYTVMLTGLDQKLPGMKVKEKFSFDVRNVNSYQ
                     NSFDMLISDLTRWKKEGYRVVLLSASRTRASRLASDLREYDLRAYCPDGTGEEHKAAP
                     GEILVTYGNIHRGFEYPLIKYVIITEGDMFGTEKKKKRRKKSNYQGKAIQSFSELAVG
                     DYVVHEEHGLGIYKGIEKVERDKVIKDYIKIEYGDGGNLYLPATRLESIQKYAGADAK
                     KPKLNKLGGTEWNKTKTRVKGAVQEIARDLVKLYAARQAQNGYQYGGDTVWQKEFEEL
                     FPYEETDDQLDAIEAVKKDMESRKIMDRLICGDVGYGKTEIALRAAFKAVQDSKQVVY
                     LVPTTILAQQHYNTFVQRMKDFPVRIDMLSRFCTPSQQKKTLEDLRKGMVDIVIGTHR
                     VLSKDLKFKNLGLLIIDEEQRFGVAHKEKIKQLKENVDVMTLTATPIPRTLHMSLAGI
                     RDMSVLEEPPVDRTPIQTYVMEYNEEMVREAINRELARKGQVYYVYNRVTDIDEVAAK
                     IQKLVPDAVVTFAHGQMHEHQLERIMTDFINGEIDVLVSTTIIETGLDIPNANTMIIH
                     DADRLGLSQLYQLRGRVGRSNRTSFAFLMYKRDKLLKEEAEKRLQAIREFTELGSGIK
                     IAMRDLEIRGAGNVLGAEQHGHMEAVGYDLYCKMLNQAVLALKGEETEEESYATSVEC
                     DIDAYIPAAYIKNEYQKLDIYKRISAIETEDEYMDMQDELLDRFGDIPKSVENLLVIA
                     RVRALAHKCYVTEVLVKEKEVKLTMYQKAKINVGGIPDLVNSYGGALKLVPGEVPVFH
                     YVERKPKGITIEDMLFKAEEILKGLRKLRM"
     gene            complement(10348..10914)
                     /gene="pth"
                     /locus_tag="ACZPCL_02550"
     CDS             complement(10348..10914)
                     /gene="pth"
                     /locus_tag="ACZPCL_02550"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR1161399.1"
                     /translation="MYIIAGLGNPTREYEKTRHNVGFDTIDVLADKLNTSVDEKKFKG
                     LYGRGIIAGEKVILLKPQTFMNLSGESVREAADFYKVDPEHIIVIYDDISLDVGQLRI
                     RKKGSAGGHNGIKNIIAHLGTQEFPRIKVGVGDKPPRMDLADYVLSRFSKEDREKMEQ
                     AFKDAAEAVEVMIAEGPDAAMNQFNGHK"
     gene            complement(11073..11729)
                     /locus_tag="ACZPCL_02555"
     CDS             complement(11073..11729)
                     /locus_tag="ACZPCL_02555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR1161400.1"
                     /translation="MYFAVTTGIRGYDMYKDAIAQVSLKDKVSEIRSKESYTVFSDLP
                     ETYTDAVLSVEDHRFYGHIGIDPIAITRAMVNDVKAGRFVEGGSTITQQLAKNLYFTQ
                     EKKMERKVAEVFMAFALERDYSKNEILELYVNTIYFGNGYYCIKDASEGYFGKEPEDL
                     TDYESTLLAGVPNAPSKYSPTVNLELAEKRQEQVVERLVACGLFTKERALETLAGSEM
                     "
     gene            complement(12098..14425)
                     /locus_tag="ACZPCL_02560"
     CDS             complement(12098..14425)
                     /locus_tag="ACZPCL_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR1161401.1"
                     /translation="MATIKDIAKKAGVAQGTVSNVLNGKGNVSSDKIRRVMEAARQLG
                     YVPNERAALLRKGTNDCLALIMPDSRARQYEDFYFSFKDYAQEHGYLVSRHLTNENSP
                     ESEATAFSEAKVKQVKGIACISAVAGTASEAAIYKNNGIFGEMEPAQNNGSDIPVLFV
                     DRKTGFPSDFIGFDYEKAGQAMAEKALQAGYRSICLLTGNPDYSSEKDFCRGFLTMME
                     GSTCQVIQIWTDSFRKYQNIMQIFNGTLPQAFFISNYGFAESAKDMCTTFYGKEEGSP
                     DIYTVAPLFTIPENDFIKYEMNYRQLGKRAAKMLIRKAEESGAEENTAQESATEENTA
                     SEKIPDNVGTEGHANWMIPGDGFQNWFVNIRKPESTQPLNIVTLDSPEAYIMKTFSRL
                     FIQKTGIDVNVCILSYDEIYEAFNSLDETSRFDILRLDVTWLSWFAQKLLRPLSEIDE
                     NIENSLGQYVEGVPEHYCRVHGQIYALPVTPSVQILYYRKDLFESPICKRTYFEQFHE
                     ELQPPKTFEEYNRIAAFFTRDLTPSSPVPYGSTITLGSTGVAGSEFLARLFAIQENLY
                     GADGQIHLDSLQCQQALAELVQLRRCTSPEYCGWWTQTARRFAEGNFAMSILYSNYAS
                     DLSAHSSHVVGNVGYSMMPGNNPVLGGGSLGVSKYCKRPKDALSFIKWMCSEPLCSAS
                     ALLGSTSPCRRTYDNYEVLHNYPWLKLTRHCFPIAKGNRLPAELSVPFDERKFLSILG
                     LAVKNAYTGIVTPKEALSDAQMQFSNYFHTSLEKR"
     gene            14777..16156
                     /locus_tag="ACZPCL_02565"
     CDS             14777..16156
                     /locus_tag="ACZPCL_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009429728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1161402.1"
                     /translation="MKKKLVLASSACLVASAMALGGCSGGSKPAETSAAAEKSEAAAD
                     ATTAAAEGGSSDAMHLSFYYPVNVGGDAAKLIEKICADFNAENPDIVVDPVYTGNYDD
                     TVTKIQTAIQGGTPPDVFVSLATQRFTMASTGMAMPLDDLIAADGDEGKAYIDDFLSG
                     FMEDSYVDGKIYSIPFQRSTEILFYNKDAFKEVGLDPEKAPATWDELVEDAKLLTNEN
                     RYGVGIALNSGSAQWTFTGFCLQNSKNGENLMAEDGKSVMFDTPENVEALQFWLDLQN
                     KYEVMAPGIVQWTDLPTQFLAGEVAMIYHTTGNLANISKNATFDFGTAFLPGNARQAA
                     PTGGGNFYISNGISEDRVQAAWKFIKFATEPERAAQWALDTGYVATRQSCFDLDIMKE
                     YYDKLPQAKIAYEQITISKPELTTYNAAEIWRVLNDNIQSAVTGDATAEEALSAAQEQ
                     ATEVLSEYQ"
     gene            16267..17163
                     /locus_tag="ACZPCL_02570"
     CDS             16267..17163
                     /locus_tag="ACZPCL_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1161403.1"
                     /translation="MKTHTKKQRELRNTLIGWLLVMPSLIFMAAFTVWPVFRSIWLSL
                     TKYKLGMSAPEFIGLKNYISLAGSSLFWKVMGNTLFFALITIVPSMVVGLFLATLVNR
                     KSRLIGFVRTSYFYPVVMPMIAIASIWMFIYMAKNGLLDQMLISMGLQPMNILSSKKT
                     VLPAMAFMYVWKEAGYLMVFFLSGIQSISTDVNEAARIDGANSWTIFRKITLPLLAPT
                     FLFVSTIALTNSFKLVDHVVIMTEGAPNNASTLLLYYIYQQGFTNFNYGVSSALTTVM
                     LLLLMVVALPRFLSQDKKIQYN"
     gene            17179..18000
                     /locus_tag="ACZPCL_02575"
     CDS             17179..18000
                     /locus_tag="ACZPCL_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1161404.1"
                     /translation="MQKKHLIGAGITAFSVILGLLWLIPLIWLIGTAFSVPSFHMTLF
                     PTTSFTLDNIKYVWNAIPFGTYYINTLTLVVCTFAVQFFTSTLAAYALAVLDFKGQAL
                     VFAVIFMQIIIPNDVLITPNFMTLSDLKLIDTKIGMMLPFYGSAMAIFLLRQHFKSIP
                     KALAEAAKIDGANTWQTIWGVYMPCAKPAYLSFAVISVSYHWNNYLWPLIVTNSPTNR
                     TLTVGLAIFAKSKEANMQWANVCAAAFIIIVPLLLAFLVAQKQFMSSFVSAGIKE"
     gene            18049..18741
                     /locus_tag="ACZPCL_02580"
     CDS             18049..18741
                     /locus_tag="ACZPCL_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024118.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1161405.1"
                     /translation="MELNFTGKGSAFYPPFKNTGAYMLSGKALYLIDCGETMFDVLYH
                     KLDLASIEDVYVILTHMHADHVGSLGTLISYFYCLFNKAIHVVYPQETIKQLLTLEGI
                     TPLGYHYHETLPENPAGLTAVPVEVKHALDMKCYGYVLSDGTETIYYSGDSSELPEDI
                     RTRFLAGEIGRIYHDTSTHDSPNPSHCYYGKMEKWIPREERHRVYCMHLDSPCEDMLK
                     EKGFSIVEVVEG"
     gene            complement(19167..19430)
                     /locus_tag="ACZPCL_02585"
     CDS             complement(19167..19430)
                     /locus_tag="ACZPCL_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161406.1"
                     /translation="MKGENPFTLTFGQKPVEFISRTDQIGKIINTFDMENPSNMVYMI
                     AGVRGSGKTVSLAVIGDHYNAKEDWIVLRLSVDMDLISDTWIS"
     gene            complement(19646..20146)
                     /locus_tag="ACZPCL_02590"
     CDS             complement(19646..20146)
                     /locus_tag="ACZPCL_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIR1161407.1"
                     /translation="MNKQYYQSRTGFITQSALIAALYVVLTMIVAPIAFGPIQFRVSE
                     VLCVLPYFLTSAVPGVTIGCFLANLLCGAASLDVVFGSIATLIGAVGSYYLGKKNKWL
                     VCVPPILSNTIIVPWVLRYAYGSTDLIPYAMLTVGIGEILAIGVLGNILLVTLERHYK
                     AVLFAK"
     gene            complement(20561..21940)
                     /locus_tag="ACZPCL_02595"
     CDS             complement(20561..21940)
                     /locus_tag="ACZPCL_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1161408.1"
                     /translation="MKYDVIIVGAGPGGIYSAYELMKKCSDKKIAVFDSGYSLKKRHC
                     PIDGEKVKACIKCKSCSIMSGFGGAGAFSDGKYNITNDFGGTLYEYIGKKAATELMQY
                     VDEINMDHGGEGTKMYSTAGTNLKKVCLQNRLKLLNASVRHLGTDINLVVLGQLYDEL
                     KEHVDFYFESPVSHIECLENGGYRVWSNDQSYDCGKCIVSVGRSGSKWMEHICKELDI
                     PTKSNRVDIGVRVELPAVLFSHLTDELYESKIVYRTEKFEDNVRTFCMNPKGIVVNEN
                     TNGIVTVNGHSYEDPEKQTECTNFALLVSKHFSEPFKDSNGYGESIARLSNMLGGGVI
                     VQRFGDLIRGRRSTPKRIEENTFVPTLSATPGDLSLVLPKRILDGIIEMIYALDKIAP
                     GTANDDTLLYGVEVKFYNMEVDVDENLESRHKGLYIIGDGSGVTHSLSHASASGVYVA
                     RKIAENLEG"
     gene            complement(22072..25053)
                     /locus_tag="ACZPCL_02600"
     CDS             complement(22072..25053)
                     /locus_tag="ACZPCL_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012873.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HIR1161409.1"
                     /translation="MPEKEVYLNILPIHHVFCINGDVFTVMRYGSVLCLNRDMARLAA
                     HIQLFQPTVIRMVPMVAKALYNKIAILSRQDTGRSMNSIKQEVLGKRLHKIISGGGYL
                     APELAANFNRLGISIAQGYGMSECSPKISSPDWSRPDKVASVGKIVDRCQVRIVDGEI
                     QVKSPSVMMGYYKEPDKTAEAITEDGWLCTGDLGYVDEENFLYLTGRKKNLIILSNGE
                     NVAPEQLENLFVDDRLIEDILVYGEDDMIAAEIYPNFKYADAAGIDHIEETVAQIIKK
                     HNEGLPSYKKIARFHLRDIPFAKTSSKKIIRSQYFSQKKQEAEEQVNVHKPENDLQQK
                     IYDAVSAVLGHSRFGIDTDLYSAGLDSLGSVLLLTDLYEKLQLSMTLNDLLEHGSVLK
                     LEALYKERAAEGKKEFEVRDIYPLTNLQLYFAYVMRGNTTANLPFLFKLDPHVNVYLL
                     KTAVERMFDVHPELKCVIQLHEGAYKNFRKDDRKVDIPLITLSDAQWEETRKGLLRPY
                     MYTENEPLYHAGIYMTESANYLFLDIAHIMGDGMTMNVLFEDINAIYAGKQVEKEKYT
                     FYEYILDEKERDAKGLRQENEAYFADLMKGFKIGKSILARCDSHDLEQGEDGVLKEHF
                     KGINRGRLLSFCRRNGISENVVFLTAFNYCIGLFSNEKDTVGTSIHSGRTDSRWNRLA
                     GPLFLTYYFRCTQKPDESVKELLQTSGKQIMKTMGCYISNLHADEMFFQYQGDILNID
                     QIGGWPVKREKVQLDALPFHLQVMWDEKGYYYELRYWKNRFDTQLLKLFMKVMEQIVA
                     AMEEEGQAANLKNRLSVDLFPLHYHIEAGKVNEAAGCELIPGVDENEQVKAYVLDESC
                     RKQPFGAWGKLYIMDHPTLGWSDKITNPYGPGMLYQTGREARIMPDGRLEFMEQGGRT
                     VMREGLTGRKFLDLYKLEQTLESADGVTKAEAYVRYGEGNKLVLQADVWGVSEADTER
                     LRAYVREHCGEALVPECISCF"
BASE COUNT         6505 a         6340 c         4843 g         7395 t
ORIGIN      
        1 tacaaggata gcacgccagt cacctgctgt tgcaagatat ccgcttaaca gaggcggtgt
       61 ggtccatgga atatatacca cgcattcatt cataaaatgc attgcagttg ctgcatatgc
      121 aataataata cctaatgcag gagttaaaat aaatggaatg atcagggaaa tattgtatac
      181 aatgggatat ccgaagataa ccggttcatt aatgttaaat aatcccggaa cgaatgccat
      241 tttggcaacg cctcttgttg ctttgttgcg accaactaag aaggtagcga tcagaaggca
      301 gatggtagat ccggaacctc ccatcatacc aaatccaggt accaaagcta cattgataat
      361 gttgggaatc gcttcacctg ctgcataagc agccatattc tgtgtcatat ttacgatcag
      421 aagcggctcc aggatggaac tgtagataac agactgatga atacctaaca gccacaagaa
      481 atttcccagg ctgtaaagga tcacgcagcc aaccaggccg gttgtaacgg tacgcagtgg
      541 ttcctggatc agagtggtta tcatctggat caggtttgta tcaaatacat tgttaagaac
      601 tgcagatata agaccaaaac cggataacag caggatgaac gggatcagaa cgttaaatga
      661 ttttccaaca gacggcggga tgctgtctcc caggtcgatc tgcagctttt tgatccctga
      721 gatccataca aaaagttctg ttgcgatcag gccgatgata acgcccgcaa acatcgcctg
      781 tgttcccaca tttccaaaag ttaaggcgcc ggatactgct acgctctcgc ctccttctaa
      841 tggggttacg ctgactacat ttggcatcat aactaccagg gctgccagtg aaacaatcgc
      901 cgctgccatg ggattatcaa agcctttgtt tcttgaaaga caataaccga tcacaggtgc
      961 aatcagcagg gagctgatat ttaaagttcc gtttgtgatc acagtgcccc agtactgggc
     1021 tcctgtaaga gtatcgccgg ataacagcca cacaaatacc gtattgttga tcagcactgc
     1081 cagacctgcc aggatataca gcggcataat ggtagcaaat gcatcacgca gggaccgtaa
     1141 atggatctca tttcccattt ttgatgcgaa gatcgtaaat ttctctacaa actttgactc
     1201 ctgaaatgat acctctttca tacttttgcc tccctttctt ctagcagtcc cttcatccag
     1261 cgggctgact ttttaagttt tctggaataa ttccccataa gatctacttc tatcatacca
     1321 tatctgtttt taaatgcatt attccaggac cagttatcga tcactcccca gtagtgatat
     1381 cccagacact ttgcaccgtc ttcaatggat ttaaagatcc actggagatg ttctgtaata
     1441 aattcgatcc tgtaatcatc ctggatctca ccggatgcat ccctgaaacg gtcttcatgt
     1501 tctactccca ttccattttc agaaacaaag aacttaagat ccggatattc atttttcatt
     1561 ttcattccaa aatcataaat accctttgga tagatctccc atcctctgta tatattcatc
     1621 tttgcttctg gccatacata tggatcagaa aatttagggt aaccgttttc atctgtcttc
     1681 tctttcggtg cctggatcct ggatggatga taataattaa agcccagcca gtctactttt
     1741 ccctgcttta atatctcttc atctcccggc cttctttccg gagccagacc tttttcttcc
     1801 aatgtagaaa gaacgtcctc tggcagatgt ccttctgcca ccagatccag ccaccaccgg
     1861 ttgttgatcc catcctccat ccgcaaagct tccagatctt ctggcgtagg atcttctctt
     1921 gtataaggcg gcgcaaaaca gttgatcagg ccgatctttg catcctctct tacatagccc
     1981 tcttcttttg cctttctgaa ttcccataca gccagactgt gggcaaggga cagatgatac
     2041 tgaacctgca ttcctctttt tgcatccttt ataaaaggat accaaaaacc gttgcggtag
     2101 cgctgatctg gttctacgat gggttcgttg aaagtaaacc agtaccggat ctctgaacca
     2161 aactcacgaa aagcggctct ggcataggct gcataagctt ccaccacatc cctgctttcc
     2221 catccgcctc tttcaaacag ataggtcggc atatcaaaat gatacaggtt cataaaaagt
     2281 tcgatctgat tttctttcgc acatttacat accttatggt aaaactgtgc tccttcctga
     2341 ttgagctttc catcttttgt aagaagtctg ctccactgca tggaagtacg gaaagaatcc
     2401 atatgaaggc ttttcagaag tttaatgtca tcctcatatt tctcataaaa attgtttcct
     2461 acatagcttc ccactttatt gtagaaatca gaaattgaaa gatctgacca ggtatcccag
     2521 atactttcca gacggcctcc tgttttataa gcgccttctg tctgaggacc tgacattgct
     2581 gcgcctacaa aaaagttttc cggaatttta agctccatgc ccatacccct ttctttttta
     2641 tatgtcattt tctcgttttg ttatgtttaa ttatttattt gttatgacat ttgttattat
     2701 ataatatttt ccaagaattg caagggtatt ttttaagttt ttaagcgaaa ttttaaaagt
     2761 gccagtttgc tgtttgcagc atactgacac tttgcacaat tactttttat atttttattt
     2821 gttttgcaca ttgattttga tatttttctg aaaatttaat gaatacttac gcttttaagt
     2881 tcaaatcgat ctgccctgtg acgggaaata gagtactcaa aaatacgtcc atcatccaga
     2941 tatgctacct gttccacttc caggacaggc aggacctgat cacctatatc cagccacttt
     3001 ttttcctgtt ctgtaggcat gtctgccctg gcgatccgat gggcactttg tatctttaag
     3061 cccagatcat tttccaaata ggaataaata gaacttttta ctacatcttc cgtaatacct
     3121 gtaataaaat ccgtaggcat gtaggtgtat tctactacat aaggtttccc atccaccaat
     3181 ctggtacggc atatatagca gacaaaagca ttttctgcca cctgcagttt ttctgccact
     3241 tctttacttg cttttttcag tccaaattca tggacatccg aagatacctt ccgtcctgcc
     3301 tggtctttac taaatccact gaactgaccg gattttgaaa agcccacctg gtccgtcatg
     3361 cccggagcca cggcttttac aaaggttcct gcaccccgtc gttttgtgat cagacctttt
     3421 gctaccagaa gctccattgc ctctcttatg gtaatacggc tggcatcata gatcttacac
     3481 atttccattt cctgtggcag tcttgtatcc ggcgggtaag ttccatcttt aattttcttc
     3541 tgtatctctt ccgcgatcat ctcatattta tacatgatat ataaactcct cttcctgtct
     3601 cgtcacgtat gtcatatcat tttataaata aattataact taccaattat aaaattacaa
     3661 ggaaaccact ttttatttta tgcatttgta tcctctgttt tttcagcagg cactaccggt
     3721 tctcccggat attcgattac ataaaattca tatccattga tcaccctggt agtaccatat
     3781 acatcttttc gtttgaaacc gcttccatag ttagcatgaa catgaccatg taaaaagtac
     3841 tttggatgat atttatccat caggtctaaa aatgccttaa atccgcgatg gggcagatct
     3901 tccatatcat ttaacttata tgccggggca tgggtcacaa ggatatcaaa gcctttgtgg
     3961 cgccacagct tccaccaaag tttatgaata cgtttcctca tcatcttctc tgtgtactga
     4021 ttaggtgagt ccggtatata ttccatggaa cctcccagtc ccagaatacg gactcccttg
     4081 tatacaaaaa tatcatcatc aatacaaatg cagccttccg gcggtttgta ttcatatttt
     4141 gtatcatgat ttccctttac atacaataaa ggggcatggc acagagttgc aaaaaaagta
     4201 agatattcag gttcaagatc tccacaggaa atgatcagat caatatcttt catctgctcc
     4261 gggtcatagt attcatacaa cagctttgat tcctgatcag caaatatcat tatgttcatt
     4321 tattcattct ccatctctgt ctgctccgtt gccttaatgc cctgaagctg catcagttcc
     4381 tgggcgcgct cattcatttc cccgatcttt ggaatatagc caactacatt ttgcgccagc
     4441 cagttcatag taatgatctc ctctgcattt aagctcttgc cctgggtgca tttggcgctt
     4501 ccatcctggg catagatcag tccgtcaaat ggatggaagc tgccggcacg aatggaattt
     4561 ttcaaaaact cgatcagccg cctggtacca tttggcatat tttcagaaca gatcacatcg
     4621 atcacatccg ctgacatacc ccaccagtag tttactgctt tcttgccttt catggcttcg
     4681 atctcttctg tactcttaaa tgcaagacgg atgatctgct catagaattt accccagtgc
     4741 cataccggca tagcaaggtt tgtcactgtt ccatcttcat ttttccgata cagaccatat
     4801 tccctggacg gatggtccgg attgatcatg tcatggccgg aaatataatg gatccctttt
     4861 tcatgtaaaa tatcttctgt atgctgcgcc tctttccttc tggcccattc cagatgtact
     4921 tccacataag gattaaccat tcttgcacca agggcaaagg cattgatatc agctaacatt
     4981 ccataggtag gctgctctgc aatatatccc agcctgtctt ccctggacag tgctgccgcg
     5041 atcgctccca taaggaattt tgcctcatac attcttgcat agtaattgct tacggaagaa
     5101 taggacatat taatggaaca gttgtaaaat ttaatatccg gataaagagc cgccgaacgg
     5161 acgctctgtc ctaacatcct ggaagtaata gtaaaaataa tggtacagcc ggcttttgcc
     5221 tctttttcaa tagcctccgc caccgcttcc tctgtatctg cctgatctac taccatagtc
     5281 gtcagttttc cggggaatgc ctgttccaga tacattcttc ccagttcatg gccatagatc
     5341 caccgggaag tttctgctgt tcttccatag ataaatccca ctttcagacc gcttggtatc
     5401 tcttcctgag gcaggaacca ttttaagatg gccggttttt tatcttcctc tacttcctgg
     5461 ggatcttgga tcagttcgat cttatttcct ctgttggcca gctggatctc ttcccagata
     5521 cgcatcaacg cccggtacat ttctctttct gtctggcctt ttacattgtc atatccataa
     5581 acttcaatat aaaccagaaa ggcatctccc tctgtaagct ttaagcggtc tccatgagcc
     5641 atggaaaagg ctttggcaaa gcgtccatag gcagatctga aatatacacg gtcctcttct
     5701 tcccacacct gatccggctt cattcccatc agtttcagaa gttctttgta tcttccttcc
     5761 ttgctgaacc acacatcaca gttagaagaa accttataaa aatccagaaa ttcataatac
     5821 agacggtttt ccagatcatc cgtcctttta gggatcagac gggtcaccgt tccgggaata
     5881 ctgtaagcac ccaggtattt cataacactg actctcttat ttccctcctg gacatagaac
     5941 cggttcataa attcataggc cacaatagga tcctgaattc cgtcctctac ctgatgattg
     6001 taaagattta accatttgat cccaaattca gaccgttccg gcaaaagagg cataaagttg
     6061 ttggcaaagg aattggaccg ttccgcagtc ttggttccca ccagttttgc cagcggaatg
     6121 tccatcgttc caaggcttac tgtggataca atatccgtat aggataaaac atcatccaac
     6181 accggcagat acgggtattc tccctttgcc actgcatcct gatattgttt tttaccaagt
     6241 tttaacgctg ctccatattc tgtatccgac atagctacct ccccgtttcc ctgatgtata
     6301 ttcatgcatt atagtaagtt atttttgctt atgctcatta tagcctcagg actactgttg
     6361 cgcaagacac ttaccattta tttacagtta aacatgtgtt atactttttt aagaagcacc
     6421 gcaacatgta caaaccgaaa ggagttccca cccatggaag accgtttagc tgttatcagc
     6481 tgcattataa gtgacccaca atctgtttcc gttatgaatg aaaaacttca tctccacgcc
     6541 tctatgatca ttgccagact gggagttccc tgtagggaat acggtgtatc cgtagtcagt
     6601 gttgtgctcc atggtaccca gaatgagatc agctcctttg ccggagacct tggaaaggta
     6661 aaaggggtaa aggtaaaatc cattcaggtg ccggtaaaca gtacatctga ttaaaaaagc
     6721 tgcggactct cacatccgca gctttcttaa tccctttaat atctcttctg ccttaaacag
     6781 catatcttca atggtaatgc ccttaggctt tctctccaca tagtggaaca caggcacttc
     6841 tcccggtacc agttttaagg cgccgccata ggaatttacc agatccggta ttcctcctac
     6901 gttgatcttg gccttctggt acatggtcag ctttacttct ttttctttta ctaaaacctc
     6961 ggtcacatag cacttatggg ccagcgcacg gactctggcg atgaccagaa gattttccac
     7021 actttttggt atatccccaa agcggtccag cagctcatcc tgcatatcca tgtattcatc
     7081 ttctgtctca atggcagaga tcctcttata aatatccagt ttctgatact catttttaat
     7141 ataagcagcc ggaatatagg catcaatgtc acattccaca ctggtagcat aggactcttc
     7201 ctctgtttcc tcgcccttta atgccagaac cgcctgattt aacattttac agtaaaggtc
     7261 atatcccact gcttccatat gtccatgctg ctctgctcca agaacatttc cggcaccccg
     7321 gatctctagg tcacgcatgg cgatcttgat accggagcct aattctgtaa actcacggat
     7381 agcctgaaga cgtttttccg cctcttcttt taaaagctta tcccgcttat acattaaaaa
     7441 tgcaaaggat gtgcggttgg aacggcctac acggccgcga agctggtaca actgggaaag
     7501 tcccagtctg tctgcatcat ggatgatcat ggtgttggca tttggaatat caagacctgt
     7561 ctcaataatg gtagtggata ctaaaacatc gatctcccca ttgataaagt ccgtcatgat
     7621 ccgctctaac tgatgctcat gcatctgacc gtgggcgaag gtaactactg catccggcac
     7681 cagtttctgt atctttgcag ccacttcatc aatatccgtc acacggttgt atacataata
     7741 cacctggcct tttcgcgcca gctccctgtt aatagcttcc cggaccattt cctcgttgta
     7801 ttccattaca taagtctgga taggtgtacg gtctaccggc ggctcttcca gtacgctcat
     7861 gtcacggatc cctgcaaggc tcatatgcag tgttcttgga ataggggttg cagtcagagt
     7921 cataacgtct acattttctt ttaactgctt gatcttctct ttatgggcta cgccaaaacg
     7981 ctgctcctca tcgatgatca gaagccccag atttttaaac tttaaatcct tagaaagcac
     8041 tctgtgagta ccgatcacaa tgtccaccat gccttttcgc aagtcttcca gcgttttttt
     8101 ctgctgggac ggggtgcaga aacggcttaa catgtcaatg cgcaccggaa aatccttcat
     8161 tctctggaca aaggtattgt aatgctgctg ggccagaatg gtagtaggca ccagatatac
     8221 cacttgcttg ctgtcctgaa ctgccttaaa agcagcccga agggcgatct ccgtcttacc
     8281 gtaaccaaca tcaccacaga tcagccggtc catgatcttt cggctttcca tgtccttttt
     8341 cactgcctca atggcatcta actggtcatc tgtttcttca tatggaaaca gttcctcaaa
     8401 ttccttctgc cacacagtat ctcccccgta ctggtagcca ttttgcgcct gacgggctgc
     8461 atacagcttt accaggtccc ttgcgatctc ctggactgca ccttttacac gggtcttggt
     8521 cttgttccac tccgtaccgc ctaacttgtt cagtttcggc tttttggcat ctgctccggc
     8581 atatttctgg atgctttcca gtctggtggc aggaagatac agattgcctc catctccata
     8641 ctcgatctta atatagtcct taatgacctt gtcccgctct actttttcaa ttcctttgta
     8701 aatgccaaga ccatgttcct catggactac ataatctcct actgccagct cagagaaact
     8761 ctgaatagct tttccctggt aattgctctt tttccgccgt tttttcttct tttccgtgcc
     8821 aaacatgtca ccttctgtaa tgatgacata tttgatcagg ggatattcaa aacccctgtg
     8881 gatatttccg taggtaacca ggatctcacc tggcgccgct ttatgttctt cccctgttcc
     8941 gtccggacaa tacgcccgca gatcatattc acgcaggtca ctggctaaac ggctggctct
     9001 tgtcctggaa gcagacagca agactacccg gtaaccttct tttttccacc gggtcagatc
     9061 gcttataagc atgtcaaagc tgttctgata ggagttgacg tttcgcacat caaaactgaa
     9121 tttttccttt accttcatgc ccggcagctt ctggtcaaga ccggtaagca ttacagtata
     9181 agaactttga agctttgcaa ggacctgggt gcctgcatag agaagctctg tctgccccgg
     9241 cagcagatag cccttttcca gtcggtggat catgctttca cgaaattcct gctctaccgt
     9301 ctcgcctttt tcctttaatc ttgccggttc atccagacag atcagagtat ctcctgccgg
     9361 gaaatagtcc agaaatgaca cagtctccgg gcagaaatac cggatatagg catccagtcc
     9421 atcccgtttc cagccttgcc ggatcccttc tgtcagttct ttgataatac cccggatacg
     9481 ggatgcttct tcaatctgat gtttggccct taaggctgct tcatattctt tttcttcctt
     9541 ttccagacgt tcaatccctg cttctaactg cttttttgtt aaaatggtct cggctgaagg
     9601 ataaatggta attttttcca gctgttccac agaccgctgg ctttccgggt cgaaactgcg
     9661 gatggagtcc acctcatcat cccacagttc aatacgcacc ggcacttcct ctgtcagcgg
     9721 gaaaatgtcc acgataccgc cacggatgga aaactggccc atgccgtcta cctgggctgt
     9781 acgctcatac cccagtgctg taaggcgttc tttccattca tccagatcca tgatctggcc
     9841 gttttccaca gtcaccgcct gctgctttaa aaataccagc ggcagcagat gatccataag
     9901 gccgtctaac gtagtgacta ctacgccttc cttctcttcc atcaggtgcc gccacactgc
     9961 aatgcgctgg cgggtcatca ggtttccgtg gatatccgca ctgtaaaaca gcagatcctt
    10021 tgccgggtaa agccatacgt tttccgtaaa gctgcgaaaa tcatcgtaga tctccctggc
    10081 tctctggtca tcataagtca ccaccagttt ccagggaaaa cccccgtctt ttcccagcca
    10141 gtacatcagg tgtaccttct gggaatcaag ggttcccgtt gcctggaccg gtccttttgc
    10201 cttttttaac gcttcctgta gattttcata ttcctgtaat tccaataacg gattttccat
    10261 gttttcccct tatatcctgt tccattatca tttcagtctg tgttgtatct atttcaacgt
    10321 ttctgtgttc aattatgcac aagatatcta tttgtgtcca ttaaactggt tcatagccgc
    10381 atctggtccc tctgcgatca tgacttccac tgcctctgcc gcatccttaa atgcctgttc
    10441 cattttctca cggtcttcct tagagaaacg gctgagtaca taatctgcca gatccattct
    10501 ggggggcttg tcacctacgc ctaccttaat tctcggaaac tcctgggttc ccagatgggc
    10561 aatgatattt ttgataccat tatgaccgcc tgcactgcct ttcttgcgga tacgcagctg
    10621 acctacatcc agactgatat catcatatat aacaatgata tgctctgggt ccactttata
    10681 gaaatctgcc gcttctctta cgctctcacc gcttaaattc ataaaagtct gaggctttaa
    10741 aaggatcacc ttttcccctg caatgatgcc ccttccataa agtcccttaa atttcttctc
    10801 atccacagat gtatttaact tgtccgccag cacatcaatg gtatcaaatc ccacattatg
    10861 tcttgtcttc tcatactccc ttgtagggtt tcccaatcct gctatgatat acataaatat
    10921 tttcctttcc aaaacacgcc tgaaagcgct agattccacc ctataggggg tatctaacgc
    10981 tcattttaat tagtcgttca cttattctag cagatatagc tttcttttac aagccgtgat
    11041 atttcacatc tctttacatc ctattttcat acttacattt cacttccggc cagcgtctcc
    11101 aatgcccgct cttttgtaaa cagtccgcag gctaccagac gttctaccac ctgctcctgg
    11161 cgtttttccg ccagctccag attgacagtg ggggaatatt tagacggtgc attagggacg
    11221 cctgcaagaa gtgtgctttc gtaatctgtc aggtcttccg gctcttttcc gaaatagcct
    11281 tcgctggcat ctttgataca ataatatccg tttccgaaat aaatggtatt tacataaagc
    11341 tctaaaatct catttttact gtaatcccgc tccagtgcaa aagccataaa tacctctgct
    11401 accttacgct ccatcttctt ttcctgggtg aaatacagat tttttgccag ctgctgggta
    11461 atggtactgc cgccttctac aaagcgtccg gcctttacat cattgaccat ggctcttgta
    11521 atggcaatgg gatcaatacc aatatgtccg taaaatcggt gatcttccac agagaggact
    11581 gcatccgtat acgtttccgg aagatcagaa aatacagtat agctttcctt ggaccgtatc
    11641 tcgcttacct tatctttcag gctgacctgg gcaatggcat ccttatacat atcatagccg
    11701 cggataccgg tagtcactgc aaaatacata gccactgcac agagcacagc tgttactcct
    11761 ttaagccagt taaacgctgc acacttcttt ttcccggtgt ttcctgctat attgtatttt
    11821 ccattgtctt ttgtcctcat ctgctcacct tctctcctgt gctatatact caggcccacg
    11881 caattatgca agcgggtttc tctcctatgc tcagacccac gcgcttatgc gctctactgc
    11941 ctgctatcgc aggcggggtc ttcgttaacc gctcgtgaaa aacaaatgtc tgcttcgcag
    12001 ccgcttgttt ttctcttcac tggttatagt atagcacatc agtataagca agtctttgag
    12061 gctcccttaa agaactctga ctaaaatctg actatttcta acgtttttct aaagaagtat
    12121 ggaaataatt tgaaaactgc atctgggcat cggaaagggc ctcttttggg gtcacaattc
    12181 cggtataagc attcttaact gccagtccca ggatgctcaa aaacttacgc tcatcaaagg
    12241 gtacagaaag ctccgccgga agccggttcc cttttgcaat ggggaagcag tgtctggtaa
    12301 gcttcagcca tggatagtta tgtaaaacct cataattatc ataggtcctg cggcatgggg
    12361 atgtacttcc cagtaaagcc gacgctgaac aaagcggctc actgcacatc catttaataa
    12421 aagaaagggc atccttcggc cgcttacaat atttagaaac acctaaactg ccgccgccaa
    12481 gcaccgggtt atttcccggc atcatagagt accctacatt tcctactaca tgggaactgt
    12541 gagccgacag gtcactggca tagttgctgt acaaaatgga catggcaaaa tttccttctg
    12601 caaaccgcct tgcagtctgg gtccaccagc cacagtactc cggcgatgta caacgccgca
    12661 gctgtactaa ctccgcaaga gcctgctggc actgcaggga gtctaaatgg atctgtccgt
    12721 ccgcaccgta caggttttcc tgaatggcaa ataatcttgc cagaaactca gaaccggcca
    12781 caccggtaga gcccagtgta atcgtcgatc cataaggaac cggtgatgag ggtgtcagat
    12841 ctcttgtgaa aaatgcagcg atcctgttat attcctcaaa tgtctttggc ggctgcagtt
    12901 cttcatggaa ctgctcaaaa taggtccgct tgcagatagg gctttcaaac aggtccttcc
    12961 tataataaag gatctgcacg ctgggcgtaa ccggcagcgc atagatctga ccatgtaccc
    13021 ggcagtaatg ctccggaact ccttccacgt actgtcccag gctgttttca atattttcat
    13081 cgatctctga caatggccgg agaagcttct gggcaaacca ggaaagccag gtcacatcca
    13141 gtcgcaggat atcaaaacgg gaggtttcat ctaacgagtt aaatgcctca tagatttcat
    13201 cataagagag aatgcacaca tttacatcaa ttcccgtttt ctggataaac agtctggaaa
    13261 acgttttcat aatataagct tccgggctgt ccagcgttac aatatttaat ggctgggtac
    13321 tttccggctt tcggatattc acgaaccagt tctgaaatcc gtctcccggg atcatccaat
    13381 tggcatgtcc ctctgtcccg acattatccg gtattttttc tgatgccgta ttttcttccg
    13441 ttgcactttc ctgtgctgtg ttttcttccg cgccggactc ctctgccttt ctgatcagca
    13501 tctttgccgc cctttttcct agctggcggt aattcatttc atatttaata aagtcatttt
    13561 ccggaatggt aaacagcggc gccaccgtat agatatccgg tgagccctct tcttttccat
    13621 aaaaagtggt acacatatcc ttggcacttt ctgcaaatcc ataattggat ataaagaatg
    13681 cctggggcag tgttccgtta aatatctgca tgatattctg gtattttcta aatgagtctg
    13741 tccagatctg gatcacctgg caggtagaac cttccatcat ggtcagaaag cccctgcaaa
    13801 aatccttttc actggaataa tccggatttc ctgtaagcag gcagatgctg cgataccccg
    13861 cctgcagtgc tttctctgcc atagcctgtc ccgctttttc ataatcaaac ccaataaaat
    13921 cagaagggaa acctgttttc cggtctacaa aaagtaccgg aatatccgag ccattattct
    13981 gggccggttc catttcccca aagatcccat tatttttata gatcgccgcc tcgctggcag
    14041 ttcccgctac tgcggaaatg caggcgatcc cctttacctg cttcactttt gcctctgaaa
    14101 atgcagtcgc ttcactttcc ggtgaatttt cattggtcag atggcgggat acaagataac
    14161 catgttcctg ggcatagtct ttaaaactga aataaaaatc ttcatactgc cttgcccggg
    14221 aatccggcat gatcagagcc aggcagtcat tggttccctt gcgcagcaaa gctgctcttt
    14281 catttggaac atagcccaat tgccttgcag cttccattac ccggcgtatt ttgtcactgc
    14341 ttacgttccc tttaccattt aatacattgg aaaccgttcc ctgggccacg ccggccttct
    14401 ttgcaatatc tttaatggtt gccatatgtg ttcctcctga tatacggtgc tggtgtggtc
    14461 tgcattttac tgtcaggtcc actctatgac cggaccttcg ttgctgctct cacttgtatt
    14521 aactataaca tatatcttaa gaaatcacaa ttctgcccca gaaattgaac cttgttcaat
    14581 cgtattccct gtaaagaaaa tgtaaaaaat gtatagcata ggtaaaattg ttaaagtaac
    14641 atgtaaattc aaaatattta ttgtgcaaaa aatcaaaata atatttgaca cgtttcaata
    14701 atgatgctat agtgaactta agagcaaatc acagacctta aaaagtctat caatgcgaac
    14761 aaaaaggagg agtatcatga aaaagaaact cgtacttgca tcatctgcct gtttagtagc
    14821 ctctgctatg gcattaggcg gctgctccgg cggttccaaa ccagccgaaa ccagcgcagc
    14881 agctgaaaaa tctgaggcag cagccgatgc aaccactgca gcagctgaag gcggttcatc
    14941 tgacgcaatg cacctgtcat tctactaccc agtaaacgta ggcggcgatg ctgcaaagct
    15001 gattgagaag atctgcgcag acttcaatgc tgaaaatcct gacatcgttg tagatcctgt
    15061 ttataccgga aactacgatg ataccgttac aaagatccag actgctatcc agggaggcac
    15121 accaccagat gtattcgtaa gccttgctac ccagcgtttc acaatggctt ccaccggtat
    15181 ggcaatgcca ttagatgacc tgatcgcagc agacggagac gaaggaaaag cttacatcga
    15241 cgatttctta agcggcttca tggaggactc ctacgtagac ggcaagatct actccatccc
    15301 attccagaga agtacagaga tccttttcta taataaggat gcattcaagg aagttggcct
    15361 tgatcctgag aaagcacctg caacctggga cgaactggtt gaagacgcaa aactgctcac
    15421 caacgaaaac cgttacggtg ttggtatcgc attaaactcc ggttctgcac agtggacctt
    15481 cacaggcttc tgcttacaga acagcaagaa tggtgagaac ctgatggctg aagacggaaa
    15541 atccgttatg ttcgatactc cagagaacgt agaagcactt cagttctggc ttgaccttca
    15601 gaacaagtac gaagtaatgg ctcctggcat cgtacagtgg accgatcttc ctactcagtt
    15661 cttagctggc gaagtagcta tgatctatca caccacagga aacctggcaa acatcagcaa
    15721 gaacgctacc tttgatttcg gtaccgcatt ccttccagga aatgcacgtc aggcagctcc
    15781 taccggcggc ggaaacttct acatctccaa tggtatctct gaagaccgtg tacaggcagc
    15841 ttggaagttc atcaagttcg ctactgagcc agagcgcgca gcacagtggg cactggatac
    15901 aggttatgta gcaaccagac agtcctgctt tgatctggac attatgaaag aatactacga
    15961 caaactgcct caggctaaga tcgcttacga gcagatcacg atctccaagc cagagcttac
    16021 tacttacaac gcagcagaga tctggagagt cttaaatgac aatatccagt ctgcagtaac
    16081 tggtgacgca actgctgaag aagcattaag cgcagctcag gaacaggcaa cagaagttct
    16141 gtctgagtat cagtaggata aaatgatact tttgaaatga agttggtgct gtcgtgcttc
    16201 acggcagcgc caatttgttt catattttta ctgttctctc gaaatctgat aaatggggaa
    16261 taaaagatga aaacacatac aaaaaagcag cgtgaactgc gaaacactct cataggatgg
    16321 ctgttggtaa tgccttccct gatctttatg gcagccttta ctgtatggcc ggtctttcgg
    16381 agcatctggc tcagccttac aaaatataag ctcggtatga gcgcaccgga atttatcggt
    16441 ctgaaaaact atatcagtct ggccggatct tctcttttct ggaaagtaat gggaaatacg
    16501 ctgttttttg cgttgatcac cattgttccc agtatggtcg ttggcctgtt tttagcaacc
    16561 ttagtcaacc gtaaaagccg tctcatcggt tttgtccgta cttcctattt ctatccggta
    16621 gttatgccta tgatcgctat tgcaagtatc tggatgttta tttacatggc aaaaaacggc
    16681 ttacttgacc agatgctcat cagcatgggc cttcagccta tgaatatctt atccagcaag
    16741 aagaccgttc ttccggctat ggcctttatg tacgtctgga aagaagccgg atacctgatg
    16801 gtattcttcc tttccggtat ccagagtatt tccaccgatg taaatgaggc agcccgcatt
    16861 gacggcgcta actcctggac cattttccgc aagatcactt tacctcttct tgcaccaacc
    16921 ttcctgtttg tatccaccat tgctctgacc aacagcttca agctggtgga ccatgtagtc
    16981 attatgacag agggcgctcc aaacaacgct tccacactgc ttctgtacta catttaccag
    17041 cagggattta caaacttcaa ctacggcgta tcttctgcac tgacaacggt tatgctgctt
    17101 ctgctcatgg tagtagcact tcctcgtttc ttaagccagg acaagaagat ccagtacaac
    17161 taaaggaggc cttacaccat gcagaaaaaa catttgatcg gtgccgggat caccgcattt
    17221 tccgttattt taggcctgct ttggctgatc ccacttatct ggctgatcgg aaccgcattt
    17281 tccgttccat ccttccatat gacactgttc ccgacaacat cgttcacact ggataatatt
    17341 aaatatgtat ggaatgccat tccatttggc acctattaca tcaacacact gacactggta
    17401 gtatgcacct ttgccgtaca gttctttact tccaccctgg ctgcctatgc actggcagtt
    17461 ctggatttca agggacaggc gctggtattt gctgtgatct ttatgcagat cattatccca
    17521 aatgacgttt tgatcacacc aaacttcatg actctgtctg atctgaagct catcgacacc
    17581 aagatcggta tgatgcttcc attctacggc agtgccatgg ctatcttctt actccgtcag
    17641 cactttaaat ccattccaaa ggcactggct gaggcagcaa agatcgacgg tgccaacacc
    17701 tggcagacca tctggggtgt atacatgcca tgtgcaaaac cggcatatct ttcctttgcc
    17761 gttatctctg tcagctacca ctggaacaac tacctgtggc ctctgatcgt gaccaactct
    17821 cctaccaacc gtacactgac cgtaggactt gctattttcg caaaatccaa agaggctaac
    17881 atgcagtggg ccaatgtgtg tgctgcagcc tttatcatca ttgttccgct tcttctggcc
    17941 ttcttagtcg ctcaaaaaca gtttatgagc agctttgtaa gcgccggtat caaggaataa
    18001 aatatcatgg ctgaactgta acaataatac ccattaagga ggaaaggcat ggaattaaat
    18061 tttaccggaa aaggcagcgc tttttatcct ccctttaaga atacgggagc atacatgctt
    18121 tccggaaaag cgctttatct tattgactgc ggagagacca tgtttgatgt gctttatcac
    18181 aaactggacc tggcttccat tgaagatgtt tatgttatcc tgactcatat gcatgccgac
    18241 cacgtaggaa gtcttggaac cctgatctcc tatttttact gtctctttaa caaagccatt
    18301 catgtagttt atccacagga gaccattaag cagcttctta cattagaagg gatcacacct
    18361 ttaggctacc attaccatga aacacttccc gaaaacccgg ccggactgac tgcagttccc
    18421 gtggaagtga aacatgcact ggatatgaaa tgctacggct atgttttaag tgatggaaca
    18481 gaaactattt actacagcgg cgactcctca gaactgccgg aagatatcag aacccgcttc
    18541 cttgcaggag agatcggccg catctaccac gacaccagca cccacgattc accaaatcca
    18601 tcccactgct actacggcaa gatggagaaa tggatccccc gggaagaacg ccacagagta
    18661 tactgtatgc accttgacag tccctgtgag gatatgttga aagagaaggg ctttagtatt
    18721 gtggaagttg tagaaggata aatccacaaa cgcagagacg ttaaaaaggc ggtccggttt
    18781 cgataaccgg atcgcctttt cgtatactct ctcctcagtt caaaatacag caaaatcccc
    18841 tggattgtta aaacattcag atagtcactt cacaatacat attgatatac tcttcaaatc
    18901 ttggcaacgc caaagtcagt ttcccatgtc ttgaaccatc aactacacct ctctccataa
    18961 gacgtttccg atacgtattt aaaagttgcg gtgatactcc caaactttcc cgtatgtcac
    19021 ctgttttatt tactccctga gaaataatat aaacgatttt gcggtccagt tctgataact
    19081 cactccatat tttttcataa gcataaaccg tcagttcttc atcaaatttt ggtaaaatat
    19141 cgtctatagt cccatcattt tcccatttaa gatatccaag tatctgaaat cagatccata
    19201 tctacactta aacgcaatac gatccagtct tcctttgcat tatagtgatc tcctatcact
    19261 gcaagagaaa ctgtcttccc agaacctcga accccggcga tcatataaac catattagac
    19321 ggattttcca tatcaaaggt atttatgatc tttccaatct gatctgtacg ggaaataaat
    19381 tctaccggct tctgcccaaa agtcagtgta aatggatttt ctcctttcat acgtattcct
    19441 ccacatctat gtttatttgt atttatttat gtttatttgt atttttcatg tttatttatg
    19501 tttattttaa tttataaata tattataccc acattaatgc aaaaaacaag gtctgcattc
    19561 aaaaagatcc ccctggcata ttcaaaatac gccagaggga tctttctcct tcacaaatga
    19621 tacattttac taatattttc caatattact ttgcaaacag cactgcttta taatgacgtt
    19681 ccagtgtcac taacaggata tttccaagta caccgatagc caggatctct ccgatcccta
    19741 cagtcagcat ggcatatggg atcaggtctg tagaaccata tgcatagcgc agtacccatg
    19801 gaacaataat ggtgttggaa aggatcggtg gaacacatac cagccatttg ttcttctttc
    19861 caagatagta agaaccaact gctccaatca gagttgcaat gcttccgaac actacatcca
    19921 gtgatgctgc accgcagagc aggttagcaa gaaagcagcc aatggtaacg cctggaactg
    19981 cactggttaa gaagtaaggc agcacgcata atacttctga gactctgaac tggatcggac
    20041 caaaagcgat cggcgcaacg atcatggtca aaaccacata aagggctgcg atcagcgcac
    20101 tctgggtaat aaagccagtt cttgattggt aatactgttt gttcattttt caattctcct
    20161 ttagttttgt tttacgagtg gaaggtcacg aacactcgat tcattttacg ccgggaatcg
    20221 gcgaaagcct tataagacct tggtttttca ggctttgcaa cgagtagaag tatagcacag
    20281 caaaaacgca aaatcaaggt cacattttga tcatctttcc aagatataaa agggggctgc
    20341 cttagaatcc catcatgctg agtaccccat aacatccaat ggcgatgagc gtaaataaag
    20401 tttcaataat ggctctggct taatccttta tcttttgctt actctgcatt aaatacttat
    20461 tttctgaacc tttcattgaa acaaaaaatc tccggcaatg gagatactat attcatacct
    20521 gcactgtcgg agatttaaat atactgctag tttatcttat ttatccttcc aggttctctg
    20581 cgatcttacg tgctacatac acgccgcttg cagatgcatg ggaaagggaa tgggtcacgc
    20641 cgctgccatc accgatgata taaagtcctt tgtgacggct ttccaggttc tcgtctacat
    20701 ctacttccat gttgtagaac ttaacttcta caccataaag caaagtgtca tcatttgcag
    20761 ttcccggtgc gatcttatcc agggcataga tcatttcaat gatgccatct aagatacgct
    20821 ttggaagaac aaggcttaag tcgccaggtg ttgcagaaag agtaggcaca aaggtatttt
    20881 cctcaatacg ctttggagtg cttcgtcttc cgcggatcag gtcaccgaaa cgctgtacga
    20941 taacaccacc gcctaacata ttagatagac gggcaatgct ttcaccatag ccgttgctgt
    21001 ccttaaacgg ctcagaaaag tgcttggaaa ctaacagcgc aaagttagtg cattctgtct
    21061 gcttttccgg atcctcgtag ctgtggccat ttacagtaac gatgccattg gtattttcat
    21121 ttacaacgat tcccttgggg ttcatacaga aggtacgtac attatcctca aacttctctg
    21181 tacggtatac gatcttgctt tcgtacagct cgtctgttaa atgggagaac agcactgcgg
    21241 gaagctctac gcgcacgcca atatccacac gattggactt ggtgggaata tccagttcct
    21301 tacagatatg ctccatccac ttgctgccgc ttctgcctac agatacgata catttcccgc
    21361 agtcataaga ctggtcattg ctccatactc tgtatccccc attttccagg cactcaatat
    21421 ggcttacagg ggactcgaaa tagaagtcca catgctcttt taactcatca tataactggc
    21481 ctaatacaac caggttaata tctgttccca gatgacggac agaagcattt aacagcttca
    21541 gacggttctg taaacaaact ttctttaaat tagttccggc tgtagagtac atcttagtgc
    21601 cttcgccgcc gtgatccatg ttgatctcat ctacatactg catcagctca gtagcagcct
    21661 ttttgccgat atattcatat aaagtaccac cgaaatcatt ggtaatgtta tatttaccat
    21721 cagagaatgc gccagcgccg ccgaaaccgc tcataataga gcaggactta cacttaatgc
    21781 aggctttcac cttttcaccg tcgattgggc agtgacgctt ctttaatgaa tatccggaat
    21841 caaatacagc aatcttctta tcgctgcact tcttcatcag ctcataagca gaataaattc
    21901 cgccaggacc ggcccccaca ataattacat catatttcat tctcaaaact cctttttacg
    21961 gtaaacatat tgatttataa cgtttcgctc ccattcccat aaaaatgggc tacctttcta
    22021 ttctaccgtg ttttgagaaa aactacaata acaaaatccc cttaaaaccg cttaaaaaca
    22081 ggaaatacac tcaggaacca gggcttcccc gcagtgttcc cttacataag cccttaagcg
    22141 ctctgtatca gcttcagaaa cgccccatac atctgcctgg agtaccagtt tattgccttc
    22201 gccgtagcgg acataggcct ctgcctttgt aacaccgtct gcgctctcta aagtctgctc
    22261 cagcttataa agatctaaga actttctgcc ggtaagacct tcccgcatca cagtgcgtcc
    22321 gccctgttcc ataaattcca ggcggccgtc cggcatgata cgtgcttccc ttcctgtctg
    22381 gtacagcatt cccggtccat aaggattggt gatcttatca ctccatccaa gggtaggatg
    22441 atccatgata tacagcttgc cccaggctcc aaatggctgt ttgcggcagc tttcatccag
    22501 tacatatgcc tttacctgtt cattttcatc cactcccggg atcagctcac agccggctgc
    22561 ttcgttgact tttcctgctt ctatatgata atgcagcggg aacagatcta cagaaagacg
    22621 gtttttcagg tttgccgcct gtccttcctc ttccatagct gccacgatct gctccataac
    22681 tttcataaac agcttcaaaa gttgtgtgtc aaaccgattc ttccagtaac gcagctcata
    22741 ataatatccc ttttcatccc acataacctg taaatggaat ggcagagcat ctaactgcac
    22801 cttttcacgt ttcaccggcc agccgccgat ctgatcgata tttaaaatat ctccctggta
    22861 ctggaagaac atttcatctg catgaaggtt ggaaatatag cagcccatgg tcttcatgat
    22921 ctgctttccg gaggtctgga gcagttcctt tacactttca tccggcttct gggtacagcg
    22981 gaaatagtag gtaaggaata atggtcctgc cagtcggttc cagcggctgt cagttcttcc
    23041 gctgtggatg ctggtgccta ccgtatcttt ttcattgctg aacagaccaa tgcagtaatt
    23101 gaatgcagtt aagaacacta cattttcaga aatgccgttt ctgcggcaga aggataacag
    23161 tctgccccgg ttaatcccct taaaatgttc ctttaaaacg ccgtcttctc cctgttccag
    23221 gtcatgactg tcacatcttg caaggatact ctttccgatc ttaaagccct tcatcagatc
    23281 tgcaaaataa gcttcatttt cctgtctgag ccctttggca tcccgctctt tttcatctaa
    23341 aatatactcg taaaatgtat atttctcctt ttccacctgc tttcctgcat aaatagcatt
    23401 gatatcctca aacagcacat tcatagtcat gccgtctccc ataatatggg caatatctaa
    23461 aaacaggtaa ttggcggact ctgtcatata aatgcccgca tggtaaagag gctcattttc
    23521 tgtatacata tatgggcgta aaagtccctt tctggtctct tcccactggg catcgcttaa
    23581 ggtgatcaga ggaatatcta ccttgcggtc atcctttcta aaattcttgt atgccccttc
    23641 atgaagctgg atcacgcatt ttaattccgg atgcacatca aacattctct ctactgctgt
    23701 ctttaacagg tacacattta catgtggatc cagtttaaat aaaaatggca ggttggctgt
    23761 tgtatttccc cgcattacat aggcaaaata cagctgtaaa ttggtcagtg gataaatgtc
    23821 acggacctca aactctttct ttccctcagc agcacgctct ttataaagag cttccagttt
    23881 taaaacagaa ccatgctcta acaggtcatt taaggtcatg gaaagctgca gtttttcata
    23941 caggtctgta agaagcagca cacttcccaa agaatccagt cctgcgctgt aaaggtcggt
    24001 atcaatgcca aaacggctgt ggcctaacac ggcagatact gcatcataga tcttctgctg
    24061 gagatcattt tccggtttat gtacattgac ctgctcctct gcctcctgct tcttctggct
    24121 gaaatactgg ctgcggataa tctttttgga agaagttttc gcaaatggaa tgtcacgcag
    24181 atggaaacgg gcaatcttct tataggaagg aagtccttca ttgtgctttt taatgatctg
    24241 agccactgtt tcctcaatgt ggtcaatgcc tgcagcgtct gcatacttaa agtttggata
    24301 aatctccgct gcgatcatgt catcttcccc atataccagg atatcctcaa tcagccgatc
    24361 gtccacaaac aggttctcca actgctccgg tgccacattt tcaccattgc ttaagatgat
    24421 caggtttttc tttctgccgg taagatacag gaaattctcc tcatctacat agcccaggtc
    24481 acctgtacaa agccagccgt cctctgtgat cgcctctgct gtcttatccg gctccttata
    24541 atatcccatc ataacagaag ggctttttac ctggatctct ccatctacga tacgcacctg
    24601 gcaccgatct acgatctttc ctacagatgc cactttatcc ggtctgctcc agtccgggga
    24661 tgatattttt ggagagcact ccgacatgcc gtatccctgt gcaatggaaa tacccagacg
    24721 gttgaaatta gcagccagct ccggcgccag ataaccgccg ccgctgatga tcttgtgcag
    24781 gcgctttccc agcacctcct gtttaatgga attcatagaa cgacctgtgt cctgtctgga
    24841 aagaatagcg atcttattgt aaagggcttt tgccaccata ggcaccatac ggatcacagt
    24901 aggctggaac agctggatat gggctgccag acgggccatg tcccggttca gacacaaaac
    24961 gcttccataa cgcatgaccg taaatacatc accgttgatg cagaatacat ggtggatggg
    25021 aaggatattt aagtaaactt ctttttcagg cagtctatgt tcagtgagtc atggtcatgt
    25081 tcg
//
LOCUS       DBMDUA010000017        24901 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1358_length_24901_cov_3.735081, whole genome shotgun sequence.
ACCESSION   DBMDUA010000017 DBMDUA010000000
VERSION     DBMDUA010000017.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 24901)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24901)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24901
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1358_length_24901_cov_3.735081"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="ACZPCL_02605"
     CDS             <1..1428
                     /locus_tag="ACZPCL_02605"
                     /inference="COORDINATES: protein motif:HMM:NF016901.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1161410.1"
                     /translation="EQINLYSSEPMVRIEKSICYSCISYEDLVSFIRNLDQKDYLSSS
                     DERIRVMITAIFFQNYVNSSKLYEQWGLSLTTKKQDTALLRQFLTRYGLELVTKKKKG
                     LSIQGDELQLRFLVIDILHPLFEFTSENKVLARFANTPLEKQTYELATEYLSCSFQEA
                     TEILSRFLSETGWSLNYPSKKFLLLFICIMKTRTVKDTSAYSYKLPLAPLNIHFTDSP
                     RQNRLYNVALSMLNFAQPLKLPFDPRLWQATENFTEQIVSELPSPFPVQDEFLRELYA
                     YFYREINLDHFHCTFVDKTVEDTKGHFPVLYEQLKRYSIIFKAAYGFTFLDEHLSTLT
                     LIFQKHILRNQTVSRNRKRIIVMTSINFERISFFLEQLREHISFQWISTLNINEIHQL
                     EQLEYDYIFCFSTRIYNILHNRKLPVIRMNFFVKDTDIDHLLEKGFSPQTHRFLTSSF
                     VVEAAGKSEGELTRFLREQYGDYFV"
     gene            1474..2058
                     /locus_tag="ACZPCL_02610"
     CDS             1474..2058
                     /locus_tag="ACZPCL_02610"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR1161411.1"
                     /translation="MNRPLDLQKKELRKILKEKRNALDPDTCRSSDTAIFKAITSWDV
                     YQKAETVFCFVGTEDEINTRPVLEDLLKRGKQVGVPKCISKGIMEVYKIRSFDDLEPG
                     KYGILEPKEGCEKISPKAIHLALIPCLSCSMKGERLGYGGGYYDRYLQQITGTQAVLC
                     RTALMCDEIPMEAHDRTIDFVICEAGVLNTKNNA"
     gene            2169..2804
                     /locus_tag="ACZPCL_02615"
     CDS             2169..2804
                     /locus_tag="ACZPCL_02615"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1161412.1"
                     /translation="MKDGKRLVITIGRQFGSGGCEVGTKLAQELGLDFYDKNILRMNS
                     DESGIKESYYYMADEKAGNKLLYKIIKSLTPEKGAPSFGSDLTSADNLFRFQSEVIRK
                     LAAEEDCIIMGRCADYVLEGMEGLVRVFIYADMDFREKRTIEKEYYAAKDAKKNIKRI
                     DRERRDYFRYYTGKEWEFPENYDLMINTAAIGTDGAVETIKNYLKVRGFEI"
     gene            2888..3760
                     /locus_tag="ACZPCL_02620"
                     /pseudo
     CDS             2888..3760
                     /locus_tag="ACZPCL_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271977.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
     gene            complement(3878..4645)
                     /locus_tag="ACZPCL_02625"
     CDS             complement(3878..4645)
                     /locus_tag="ACZPCL_02625"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF013152.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HIR1161413.1"
                     /translation="MKISDNGLNIIKKFEGCRLTAYQDTVGVWTIGYGTTNADKAITG
                     TTICQGLRISQETADEWLRQSVDKKYGPKVDKYSAYNWTQPEFDALVSFAYNIGSIDG
                     LTAKGTRTRSEIAAKILEYNKAGGKVLSGLIRRRQEERKLFLTPVTVKTGWQQENGGR
                     RFYLKDGSGKYVSNDWHKDGELWYWFNGAGMMVHDTWYQYKGSWYYLGSDGAMLKGLQ
                     TINGKWYYLDQDGRMATDPVTLTPDQDGALQYPGLVK"
     gene            complement(5059..5322)
                     /locus_tag="ACZPCL_02630"
     CDS             complement(5059..5322)
                     /locus_tag="ACZPCL_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR1161414.1"
                     /translation="MDFGIGSVTAITAICYLGGMACKATTKVKDEVIPVVCGLTGGIL
                     GVAGMYLMPEFPATDVINAAAIGIVSGLAATGAHQVIKQASKK"
     gene            complement(5325..5666)
                     /locus_tag="ACZPCL_02635"
     CDS             complement(5325..5666)
                     /locus_tag="ACZPCL_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161415.1"
                     /translation="MESIMQYISVHWVSWVFGIISVLLSGAYHKLSKQLKAERARTNA
                     INAGVLALLHDRLYQACTFYLKRKYCTLEDRDNLEYMFRPYKALGGNGTGEDLYNRCL
                     ALPYEPAEQEV"
     gene            complement(5670..5849)
                     /locus_tag="ACZPCL_02640"
     CDS             complement(5670..5849)
                     /locus_tag="ACZPCL_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1375 family protein"
                     /protein_id="HIR1161416.1"
                     /translation="MLRLLLFLLLRKEVETMAIIYATLIVKGKKTYAQVPEKIKPQVK
                     QVLIDLECEDLITEE"
     gene            complement(5842..5943)
                     /locus_tag="ACZPCL_02645"
     CDS             complement(5842..5943)
                     /locus_tag="ACZPCL_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1375 family protein"
                     /protein_id="HIR1161417.1"
                     /translation="MARVYADLIRKGKINIEDVPVKIRAEVEALLNA"
     gene            complement(5943..6371)
                     /locus_tag="ACZPCL_02650"
     CDS             complement(5943..6371)
                     /locus_tag="ACZPCL_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161418.1"
                     /translation="MESIKIGTQSYELVADGYQLQQDGGCIIFQPGEQTFEEVETVMS
                     GVKSILLLDENGDTMASRTDLVYAGRMTRQKDYVIRVEKNQTGTDSEGNAVYEYKDIT
                     GTVLIAEFRLPDLREKYAALEEEVTNTQLAIVELYEGGEA"
     gene            complement(6763..7332)
                     /locus_tag="ACZPCL_02655"
     CDS             complement(6763..7332)
                     /locus_tag="ACZPCL_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161419.1"
                     /translation="MKLSSFTAYVKQLWKNKPDTSTPLSAERLTHMEEGIKGNSDAIE
                     AIAAAVVSQIVNDPDKIASMAALYSVNQKIGDVSKLPDSAADVVTAIAKQNSNLDSGY
                     FKIKVKTTTIVLIIEEFTFTNGVATKTLQSIFGSIPTYASGICQTKVEDSGVYNFTAV
                     KDGNNLKIATAGSTFSGKKWVTMIIFGTA"
     gene            complement(7329..7553)
                     /locus_tag="ACZPCL_02660"
     CDS             complement(7329..7553)
                     /locus_tag="ACZPCL_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161420.1"
                     /translation="MADSIYIQSVSITPNPVTAGGKIKIEVEIYTLCPQTTLYPATTL
                     YPGEDLFTLHPDTDLYPSKDIYPTEGGIET"
     gene            complement(7553..7819)
                     /locus_tag="ACZPCL_02665"
     CDS             complement(7553..7819)
                     /locus_tag="ACZPCL_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161421.1"
                     /translation="MQRVHFYVGENKSIGLRIHARDQAPFTIRDATWELKGNYETEAQ
                     GECEINGDVIRAMIAPQKRVTYRLYFTYKVAEEILMECIEVVAE"
     gene            complement(7795..8124)
                     /locus_tag="ACZPCL_02670"
     CDS             complement(7795..8124)
                     /locus_tag="ACZPCL_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HIR1161422.1"
                     /translation="MVKRVFGRVDGCEVELNRSEGDWWNVPVPFDTDGEYVVEILAED
                     EAGNQAYIAKMLFVVNTALLCAHVEPVPYYGQLLETEWEAELVAPQIYTELLVEGGKR
                     CNGSISM"
     gene            complement(8137..9051)
                     /locus_tag="ACZPCL_02675"
     CDS             complement(8137..9051)
                     /locus_tag="ACZPCL_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR1161423.1"
                     /translation="MAVKTVQAIINGVTTTLTLNSSTGKYEATITAPSKSSYTINDGH
                     YYPVTVKATDVAGNTTTKTDSDTTLGVSLKLKVKEKVAPAITITSPTAGSYITNNKPT
                     IKWKVTDADSGVNPATIGITIDSGSKVTGDSITKTAVTGGYECTYTPTTALADGSHTI
                     KIDASDFDGNAATQKTVTFKIDTVPPTLSITAPADKLVTNKTAVTVTGTTNDATSSPV
                     TVTVKLNSGTAETVTVGSDGTFSKALTLVTGTNTITVVAKDSAGKTTTVTRTVTVDTT
                     APVIKSVTINPNPVDCGKTYVISVEVTD"
     gene            complement(9051..9203)
                     /locus_tag="ACZPCL_02680"
     CDS             complement(9051..9203)
                     /locus_tag="ACZPCL_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161424.1"
                     /translation="MYNLSEEQRATLLYIFDHLIVTGPDQAALLSNAAVVVRGLEKTD
                     KKKEIK"
     gene            complement(9196..11295)
                     /locus_tag="ACZPCL_02685"
     CDS             complement(9196..11295)
                     /locus_tag="ACZPCL_02685"
                     /inference="COORDINATES: protein motif:HMM:NF025253.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HIR1161425.1"
                     /translation="MLNVDERLKELYRADSTDKQLILDFYHKGEDEPYLRLSSSNIKA
                     ETMELDEALSSNENLEFGSCEASQFKITLLNVTEVVKEARMEVYQILEGIWPEAGLFP
                     GDDIYPNGYRMPLGVYIIKSAEKETDRKYLDIVGLDQMSLFDVNVAQWYNNLSFPMTL
                     KEFRSSLCQYVGVTEKVPSYLPNDSILIEKTMSVEELSGRDTLIACEQMNGVFGHFDR
                     EGILQHIALQPNYILAPAENLYPSDELYPLVPGEMNEQVYDETISQNLYKSCVFEDYT
                     VKAIEAVQIRQEEEDIGAIYGTGNCLVVEGNFLLYGKGADELQQIAAGIYGMVSSRPY
                     VPYECNLLKGLPYLELGDAGLIKSEEGTIVSYIIKRTMKGIHALQDTYSATGEEIRKE
                     EQGTNADIIRLKGKAAYLKKNVDEVSANLVDLEKRTEAKLTITAEQIAAEVKRASAAE
                     GELSSQITMTAENIKLMVKKGEVSAQLSIESGGIDIKGNRFSWTSTYSSLTADGKLTV
                     VEGLFKGSINVGDGQFTVDQNGKVLAKNIEIGTTATGATIYGETVLASRFTCRDTFSV
                     DCYASMADIGANTIGCDKLRANTIIGTIDEYSDRRLKENIHKVDTGTALQIIKQLQPV
                     SYNMKRTDHEGIGFIAQDVRRICRKQGLNLPLYGHSGRYFTIPYTNYIPLLVAAMQSQ
                     QEEIDRLKSLIRKEAHV"
     gene            complement(11307..11717)
                     /locus_tag="ACZPCL_02690"
     CDS             complement(11307..11717)
                     /locus_tag="ACZPCL_02690"
                     /inference="COORDINATES: protein motif:HMM:NF042749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6711 family protein"
                     /protein_id="HIR1161426.1"
                     /translation="MAFKGWLIKFGNTVLPNKYLEKYKSTPNQRLELDASRDATALLH
                     RQTSPNYKTSLTVPIRKLYLGEKIVVKAIIDAGIVSGGERERKVSVTYWNDEEMDYKS
                     GVFYIADIEYTISHVNERKLDMVYEPFDIQLTEY"
     gene            complement(11730..14786)
                     /locus_tag="ACZPCL_02695"
     CDS             complement(11730..14786)
                     /locus_tag="ACZPCL_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR1161427.1"
                     /translation="MAADGSLKFDTKIDTSGLEKGTGTLEKAFDRLTKAVDRLSDNIS
                     SAFNNVGQAAETAAAQSSKAAEGIDQVTESAKQAERQTKSLQEQMDAIKVDRGEYQEQ
                     EPTPARRVKVDDPSAYGYDPAAIEFIDKYVSGEEEAEKATNEFAQKISELKQELKDLE
                     SQGMYFGDEKYDEAYLKLEKVKQALKDYKQELTNPTPDAVIFPADSLQGKIDRLKKEL
                     KDLESQGKSFGDALYDSTYKALNQAQSELNTYKKNLTTPVKIPVQFDADSFEGQKEAL
                     RSKLLGMEQQGISLGDADYDQTYVQLQQVIQAENEYKKSLLNADAGQKKAKASADKLR
                     DSVNGAGKAAKNSGKGMQLLGRISRMMMMRFVMQAVMAVMSATKEGFQNLARYSGSAN
                     QTLSGLSSSLLYLKNSLAAGFAPILSVAVPAITALIDAIAQALAWIGQLVAALTGKST
                     FVKAKKTQEDYAKSLKKTGSAAKDAKNSLAAFDKLNVLSQNNAGSGGGGSGTDPSQMF
                     EMVAVSSSLSKALDALKKKWSDLSSLFTKGFKVGLGDTTSRFATIQKGLQSIKESLSD
                     IFSDPRVQAAASTWGNKMVYDLGVIAGAVASVGITLAANLVGGTAKYLEEAQERIKQY
                     IIDMFDITGDIADIVANFSAAFAEVFSVFADENGQTFTANLIGFFSNSFMGLTEVFVK
                     LGRDLLNALLTPLTNNTAGFKQAFDGLLGVAAQIMGDLKDLFTDAFDQINQTYDEHVA
                     PMFDAFTEGLTEIHKSALEAFETYILPALQKVADKFTEVKSQYLQPFIKSFVELFGNV
                     ADTLTVLWNQVLQPLLNWIVQSFAPLIGAAIENVGGFFTALLAAVSTAAQGVTDALNG
                     ILEFIQGVFTGDMEKALNGIKDIFKSVFNGIISTVEVAINHIVEGLNGISFDVPDWVP
                     LAGGQHFGFNVSSMKLPRLATGTVVPRQAGEFAAILGDNNREAEVVSPLSTIKQALLE
                     ALKEAGAGLGGDIQLMINLDGKVVYENVVKRNRLARKQTGKNPLLV"
     gene            complement(14834..15400)
                     /locus_tag="ACZPCL_02700"
     CDS             complement(14834..15400)
                     /locus_tag="ACZPCL_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp15 family bacteriophage protein"
                     /protein_id="HIR1161428.1"
                     /translation="MIRLGQLPVILMVGEKEWKIRTDYRDILVIMQAFNDPELTVEEA
                     YIVMCKILYEDWPDMPSELYEESAKKALWFLDCGQEDEEDIMPVKVMDWEQDEPILFP
                     AINQVAGCEVRSVPHVHWWTFVGYFMEIREGIFSTVLGIRQKMAKGKHLEKWEREFRR
                     NNKKICDLKKRYTKEEQEEIDYWNKLLG"
     gene            complement(15397..15843)
                     /locus_tag="ACZPCL_02705"
     CDS             complement(15397..15843)
                     /locus_tag="ACZPCL_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161429.1"
                     /translation="MESLKFDEGYKEYMVNEDPDRVIRINPSDLNMWQRCMDEMKKLE
                     QVKDELQGNVKLLEDGTVDPMDENASIEWKKAEQGVKDCFNAIFNADVYDILFNGQSP
                     FSPVKGGKLLFESVMDGLMPIIKKNMKAGVEASNKRIQKYTVGYTK"
     gene            complement(15855..16373)
                     /locus_tag="ACZPCL_02710"
     CDS             complement(15855..16373)
                     /locus_tag="ACZPCL_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161430.1"
                     /translation="MSDKTPVAGQKIKRKFMGNFIDSALPGTKETAYVRLGKDLEEYN
                     VEMNANVDTKNNILGETSVTLDSYQPQATADPFYAVVGDPMFERLQGIIDERQTLDDL
                     KTTVVEVHLWESASGSAGSYVAYCEDAIIEVSSYGGDTTGYQIPFNVHHTGNRVKGTF
                     ALETKKFTPDSE"
     gene            complement(16374..16829)
                     /locus_tag="ACZPCL_02715"
     CDS             complement(16374..16829)
                     /locus_tag="ACZPCL_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161431.1"
                     /translation="MTIIEYMRQKLTEYPKISEFLTGDDIHIDFTEPDPINYGLSSNG
                     DTLVKEDILGNQVRRHNFVMYAVGQSFTDYSRLANSNFLLELAYWLEQLPEEEGIEVN
                     EGDEVKQATFLKATTANAMSMGLMGDTVDQGVMYQLQIYAQYKVESEEF"
     gene            complement(16826..17251)
                     /locus_tag="ACZPCL_02720"
     CDS             complement(16826..17251)
                     /locus_tag="ACZPCL_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HIR1161432.1"
                     /translation="MKIVIKMLSTAEMRRRHKVAENGPVQAAIDSECMRYMSDYMPRR
                     QAGELKHLMVAATTIGSGQINTPGPFAHYLYEGILYVSPTTGSAWAKKNEIKVPTGKE
                     LTYAGAPMRGKKWFERMKADHKDDILQAAQAVLSRGGRI"
     gene            complement(17263..17643)
                     /locus_tag="ACZPCL_02725"
     CDS             complement(17263..17643)
                     /locus_tag="ACZPCL_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6751 family protein"
                     /protein_id="HIR1161433.1"
                     /translation="MFTNADVTLYLYHKCGKDMRYIRIPVEDVYWEDVRQSTFLKTGQ
                     RDAASVLLVIPLESITEPLTFTQGRDLAVKGIVEDEIDCSSQEVMSKSLAALKASHGY
                     VTVTTVDGRLYGSEAVQHYELSCK"
     gene            complement(17643..18014)
                     /locus_tag="ACZPCL_02730"
     CDS             complement(17643..18014)
                     /locus_tag="ACZPCL_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HIR1161434.1"
                     /translation="MVYADEEFYKNEYLLGRKAAISSGFPFYARQASQIMDQYTFGRL
                     TIMEDVPEEAKLCCCELAEVICRQEKTEKNASGKTSEKIGTYSASYGSSQELTEAAIR
                     KQNQIVKKWLENTGLCYQGVC"
     gene            complement(18138..19229)
                     /locus_tag="ACZPCL_02735"
     CDS             complement(18138..19229)
                     /locus_tag="ACZPCL_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIR1161435.1"
                     /translation="MPVHITSRSDAEAIIREQVVSTIFQDAPKQSVFMSMARKLPNMT
                     SDQTRIRVLDFLPTAYWVDGDTGMKQTSRQAWDNVYLDAAELAVIVPIPEAVLDDAEF
                     DIFGEITPRVNEAIGQRVDSAIIFGVNRPRVWQNDIITLARQAGNNVAPGSTPDYYNL
                     LLGENGVISKVEEDGYMATGALAAMGMRAKLRGIKATDGTPIFKSDMQGSTNYALDGA
                     PMYFPQNGAYDNSIAQLIVGDFKQAVYAIRQDVTVKILDQGVIQDPITKDIVYNLAQQ
                     DMVALRIVFRMGWALPNPATRMDEDRVGCPFAYLEPATPVTTQTVTFTVKDNAGSPEA
                     IEGAIVDVNGSRLKTNASGEAVFNLRPGT"
     gene            complement(19245..19778)
                     /locus_tag="ACZPCL_02740"
     CDS             complement(19245..19778)
                     /locus_tag="ACZPCL_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HIR1161436.1"
                     /translation="MKRKFLEDMGLSKEQVDNIMAENGRDIEAIKTERDNYKTQLDTA
                     QTTLKSFEGVNVQDLQGKVIKLTADLAAKDAEYQKQIADRDFNDLLKTTAEGYKPRDL
                     KAVMPFMDVEKLKASKNQEADIKTALEAVKKDNAYLFQDTQIPKVVSYTPGSGGKGTE
                     DTKTRANEALRSIFGRE"
     gene            complement(19887..20048)
                     /locus_tag="ACZPCL_02745"
     CDS             complement(19887..20048)
                     /locus_tag="ACZPCL_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161437.1"
                     /translation="MKEGDFMGLISWIRRRFFRKKECCHHYRKHWSRASGPYGGYVRR
                     CTKCNKIEQ"
     gene            complement(20245..20424)
                     /locus_tag="ACZPCL_02750"
     CDS             complement(20245..20424)
                     /locus_tag="ACZPCL_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161438.1"
                     /translation="MEDDRFVKCPLVDEMIEDIDCIENVDAVDGVLKMDKVPERFKEK
                     ADWKGICKNCKWHSY"
     gene            complement(20414..21964)
                     /locus_tag="ACZPCL_02755"
     CDS             complement(20414..21964)
                     /locus_tag="ACZPCL_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor capsid protein"
                     /protein_id="HIR1161439.1"
                     /translation="MTPEELEKLPKPLERTMTALEMDIMLEVVNRIRECSQITPVTDW
                     LLNRMTAIGMSKKRIKEILREGVKTAGIDIDEIYETAARSDYVRNSEIYKAAGMDAIP
                     YEDNDWLKQVVQAVKDQTTDSLRPMENITKTTGFNVPMGNGKKVFTPMSEYLERSLDE
                     AVMKITTGAKTYSQAIGDVIDEMTSSGVRVVDYASGRSDRIEVAVRRAVMTGIAQMTD
                     KVNEHNAKELGTDYWEVEWHLGARNMGTGYMNHQSWQGKVYSSAEMRTVCGLGEMLGF
                     AGINCYHIRFPFIPGISKRKYTDEWLVEQNRKENEKKSFHGKEYDTYAALQYQRKLER
                     TIRKQKQDIKLLEKARADKDDLTAARCRKRLTEKTYVEFSKAMGLRQQRERLKVGEAN
                     PTKEELEAIEKRKRIAIIKSELKELGLRGKINLEPSKVDFEKLSFDSEHINDERQHEV
                     TFDEAKGFIRRASFSETVWKGQFERYYSEDGAAYVRTSDAFIRTAFKREEYSDNILKA
                     LEIVRHGR"
     gene            complement(21970..23349)
                     /locus_tag="ACZPCL_02760"
     CDS             complement(21970..23349)
                     /locus_tag="ACZPCL_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIR1161440.1"
                     /translation="MKFSKMLAAITQVLNQDSDTQVDVCMTSEMARRIELWTAMYEDN
                     APWVDRKKVKSAQLPAAIASEVARLVTLEMKSEITGGSSAAYLNDQYQRKVLKSIRRY
                     VEYGCAKGGLILKPYVTKTGLAIQYVQADCFFPLAFDDSGQIQQCVFTEQFRKGQKIY
                     TRLEVHTLQGEQIRITNRAFVATNDYSLGSEISVNSMDRWSELMPEAVLEGADRLLFG
                     YFKVPLANANDTGSPLGVSVYSRAVELIKEGDRRYSNICWEYEGTQLAVHVATSMLKY
                     NHDLDKFEYPGGQDRLYRNVEYNTGASDKPFIDTFSPEIRDTALFNGFNNQLKLIEFA
                     CCLAYGTLSDPQNVDKTATEIKTSKQRSYTFVSDTQLALQTALEDLVYAMNFWASLYG
                     LVPPGNDYQVSFDWDDSIVVDAEADRQTDRADVAMGVMSLAEYRSKWYGETLEEAQKN
                     LPEPANVEE"
     gene            complement(23361..24617)
                     /locus_tag="ACZPCL_02765"
     CDS             complement(23361..24617)
                     /locus_tag="ACZPCL_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit domain-containing
                     protein"
                     /protein_id="HIR1161441.1"
                     /translation="MPFSDKQQEFFRNANHRWNIKVGATRSGKTYMDYYVIPKRIRAR
                     AEKEGLVAILGVSKGTIQRNIIEPLQRIWGTKLVGDINSQNICPMFGEDVYCLGAEKV
                     SQVSKIRGSSLKYCYGDEVVDWNQDVFNMLKSRLDKPYSCFDGACNPDAPQHWFKKFL
                     DSDADIYCQKYEIFDNPFLSRVFVDELCKEYKGTVLYDRYIRGLWVAAEGSVYKLMCD
                     ATSSGGINPYAIYEKPKSLLQINIGVDFGGSGSGHAFVATAYSRAYQSITALASERHM
                     SVNGSIDPDKLGELFVDFCLKIINLYGFITVVYCDSAEQTLIAGMRTAVRKAGLGWIR
                     IENALKTTINDRIRFMQRMLGQHRFFYVKDQCQSLEDALTTALWDEKKSLVEDVRLDD
                     GTSDIDTLDAFEYTFERDISRFIRYE"
     gene            complement(24619..>24901)
                     /locus_tag="ACZPCL_02770"
     CDS             complement(24619..>24901)
                     /locus_tag="ACZPCL_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HIR1161442.1"
                     /translation="EKDGNVIGERWEVQQAWDKQGKFLQAQARAQSELRSLIKQYDEL
                     LHKRWDLASDEQKARIAQIKAQTDKLKGTDNEEELSRLDQVLSEIKGVV"
BASE COUNT         6348 a         6295 c         4705 g         7553 t
ORIGIN      
        1 gaacagatca atctttactc cagtgaacct atggtccgga tcgaaaaaag tatctgctac
       61 agctgcatca gctatgaaga cctggtttcc tttatccgca acctggacca aaaggattat
      121 ctcagctcct ctgatgaacg gatccgggtg atgatcactg ccatcttttt tcaaaattat
      181 gtaaactcat ccaagcttta tgaacaatgg ggactttccc ttactacgaa aaaacaggac
      241 acagcactgc tgcggcaatt tttaacgcgc tatggcctgg aactggtgac aaaaaagaaa
      301 aaagggcttt ctattcaggg cgatgagctc cagctgcgtt ttctggtcat tgacatcctc
      361 catcctctgt ttgaatttac atcagaaaat aaggtacttg cccgttttgc aaatacgcct
      421 ctggagaagc agacctatga gctggcaacg gaatatctat cctgcagttt tcaggaagca
      481 accgaaattc tttcccgttt tctctcagaa acaggctggt ccctaaatta tccctccaaa
      541 aaattcctgc tgctttttat ctgtattatg aaaacacgga ctgtaaaaga tacatctgcc
      601 tactcttaca aactgccttt ggctccttta aacatccatt ttacagactc tccccggcag
      661 aaccggcttt ataatgttgc cttaagtatg ttaaatttcg cccagccttt aaaacttcct
      721 tttgatccaa ggctctggca ggcaacggaa aatttcacag aacagatcgt aagcgaactt
      781 ccttctccct ttccggtcca ggatgaattt ttaagggaac tatatgccta cttctaccgt
      841 gagatcaatc tggaccattt ccactgcact tttgtagata agaccgtaga ggatacaaaa
      901 ggacattttc ctgttttata tgagcagtta aagcgttaca gtattatttt taaagctgcc
      961 tatggcttta cgtttctgga tgaacattta tccacgttga cccttatttt ccagaaacat
     1021 attttaagaa accagaccgt ttccagaaac agaaaacgga tcattgttat gaccagcatt
     1081 aactttgaac gtatcagctt ctttttagag caactgcggg aacatatctc ctttcagtgg
     1141 atcagcacct taaatatcaa cgagatccac cagttggaac agctggaata cgattatatt
     1201 ttctgctttt ccacccgtat ctacaacatt cttcacaacc ggaaactccc tgtgatccgt
     1261 atgaactttt ttgtaaagga tacagacatt gaccacctgc tggaaaaagg attttctcct
     1321 cagacccacc gtttcctgac cagcagcttt gttgtggaag cagccggaaa atcggaagga
     1381 gaactgaccc gatttttacg ggaacagtat ggcgattatt ttgtataact gaatttcatt
     1441 ttgaaaaaat tctattctga aaggaaacca actatgaaca gaccattaga cttgcagaaa
     1501 aaagaactcc ggaaaatatt aaaagaaaaa aggaacgctc ttgatccgga tacctgccgt
     1561 tccagtgata ctgccatttt taaagccatc acctcctggg atgtatacca aaaagctgaa
     1621 actgttttct gttttgtagg cacagaagat gagatcaata ccaggcctgt actggaagat
     1681 cttctaaaaa gaggaaaaca ggtgggtgta ccgaaatgta tcagcaaagg cattatggaa
     1741 gtatataaga tccgttcttt tgatgactta gaacctggaa aatacggaat tttggagcca
     1801 aaagaaggct gtgaaaaaat ctcaccgaaa gccatccatc tggcactgat cccctgcctt
     1861 tcctgcagca tgaaaggaga aagattggga tacggcggcg gatattatga ccggtattta
     1921 caacagatca ccggtaccca ggcagtcctc tgccgcactg cccttatgtg tgatgagatc
     1981 ccaatggagg cccacgaccg gaccattgat tttgtaatat gtgaagcagg ggtattaaat
     2041 acaaaaaata acgcctaagt tttcttaaga atattagttt tttgtcaaat taacagaaaa
     2101 acacgttttt tgcctcttcc atatgctata ctaaatgcat ctaatacaat aaaatgggag
     2161 gcaggcatat gaaagacgga aaaagactgg tcattaccat cggaagacaa tttggcagcg
     2221 gcggctgcga agtaggaacc aaactggccc aggaacttgg actggatttt tatgacaaga
     2281 acattctgcg catgaactca gatgaaagcg gcatcaagga aagctattac tatatggctg
     2341 acgaaaaagc cggaaataag ctgctttata agatcatcaa gagcctgact cctgaaaaag
     2401 gagctccctc ttttggttct gacctgacat ctgcggacaa cctgttccgc ttccagtcag
     2461 aggtgatccg caaactggca gctgaagaag actgcattat catgggtcgc tgcgcagatt
     2521 acgttttaga gggcatggaa ggtctggtac gtgtatttat ctatgcggat atggatttca
     2581 gggaaaaacg caccattgaa aaagaatatt acgcagcaaa agatgcaaag aaaaatatca
     2641 agcgcatcga cagggagcgc cgtgattact tccgctatta tacaggaaaa gaatgggaat
     2701 ttccggaaaa ttacgacctg atgatcaata cagcagccat tggaacagac ggtgctgtgg
     2761 aaaccattaa aaattatctg aaagtccgtg gatttgagat ttaaaaaata cagataaaaa
     2821 agctgtcata aaataaaagt gccttctcat atactgaaaa cgacggtata aaaagaaggc
     2881 attttttatg gcaaatggac aggattttat atccagaggc aatttacaga tccaggtgat
     2941 caacatccgg aataattttc ccatacaaaa cgcaaaagtt tccatcagct ccaaaggaga
     3001 accggaacgt gtgctggaac agctgactac agactcttct ggacagacgg aaaacatttc
     3061 tcttcctgcg cctcctgagg aatacagttt agagcctggt atctaccagc cttattctga
     3121 atatgatgtg ctggtggaag cagaaggctt taaaccctta aatatctccg gcaccgagat
     3181 tcttgcagga acacaggcca tccagcctgc agcacttaca ggtgatgaag gcggcacacc
     3241 tcctgaagac ccagtagtca tcccagacca cactttattt ggaaattatc ccccaaagat
     3301 tgctgaagcc gaagtaaaac cggtaaacga aagcggtgag atcgtattaa gccgtgtggt
     3361 ggtcccacag actattgtgg tccacgacgg agcgcctaca gactccaccg ccaaggatta
     3421 ctatgtacca tacagagatt atattaaaaa tgtggcttcc agcgaaatct actccacctg
     3481 gccccaaagt accattactg ccaatgtact ggctatcatg tcctttacct taaatcgtgt
     3541 atacacggag tggtacagaa accagggcta tgattttacc attacctctt ccactgcctt
     3601 tgaccataaa tggatctacg gcaggaatat ctttgaaagc atctcccagg tagtcgatga
     3661 gatctttgac agcttccttt caagacccgg tgtacgccag cccatattga cccagtactg
     3721 tgacggacgg caggttcagt gtccacggtg gatgaccaga ttgtaagtgc gtatagggga
     3781 tttttcctta gattttggca gaatttttct atattttcat cataaatcag gtgttgaaat
     3841 ttggcaagat ataaccgatg atcagatgtg tgattttcta ttttacaagg cctggatact
     3901 gtaaggcgcc gtcctgatcc ggtgtcaatg ttaccggatc cgttgccatg cggccgtctt
     3961 gatccaggta gtaccacttg ccgttaatcg tctgcaggcc tttaagcata gcaccatcag
     4021 atccaaggta ataccaagac cctttgtact ggtaccaggt gtcatggacc atcatgccgg
     4081 caccgttgaa ccaataccag agttctccat ccttgtgcca gtcattagaa acatattttc
     4141 ctgagccgtc ttttaaatag aagcgccggc ctccattttc ctgctgccaa cctgttttta
     4201 ctgtaacggg agttaaaaag agcttcctct cttcctgacg tctgcggatg aggccagata
     4261 atacctttcc gccagcttta ttatactcca ggattttggc tgctatctca gaacgggtac
     4321 gagtaccttt agccgtcaat ccgtcaatac ttccgatgtt atacgcaaaa gataccaagg
     4381 catcaaactc tggctgcgtc cagttgtaag cactatactt gtccactttt ggaccatact
     4441 ttttatcaac agactggcgc aaccattcat ctgccgtttc ctggctgatc cgcagacctt
     4501 ggcagattgt tgttccggtg attgctttgt ctgcgtttgt ggtgccgtat ccgatggtcc
     4561 agacacctac ggtatcctga taagccgtta gtcgacaacc ttcaaacttt tttatgatat
     4621 ttaatccatt atctgatatt ttcatagcat ttcctttctt ccaaatggta tcttgaaaag
     4681 attttctgca tatgctataa tgccgttagg caaaaagaaa taagtttcca tgttggacac
     4741 aaaagcgaaa gcccctgagt ggtcgagact caggggcttt cttccttttt aagcagttag
     4801 gcatctgcag gctggttacc gactatttgc cgccgtccaa ccatttgatg atgtagtggc
     4861 aaactacacc agccgcaaca gcgacaataa aagaaataag attttccatg tcagacaccc
     4921 cctttcctta ccgggtatag gggcggtaac ctagacatta taacatatgc atgattttta
     4981 ttctactgat ttattgcgac gtcgcaaatg gcagccatga cccggactgc ctgcgggaga
     5041 tagtcggatc acctccttct acttcttgct tgcctgtttg atgacctggt gcgctccggt
     5101 tgcggccaga ccggatacaa taccaatagc tgcagcgttg atcacatctg tggctggaaa
     5161 ctctggcata agatacatgc cagctactcc caggatgccg ccggtcaatc cgcatactac
     5221 cgggataacc tcatccttga ccttagtggt tgccttacat gccatgccgc ccaggtaaca
     5281 gattgctgtg atcgctgtta cgcttccaat tccaaaatcc atatctatac ctcctgttct
     5341 gccggctcat atggtaaagc cagacatctg ttataaagat cttctccggt tccatttcca
     5401 cccaatgctt tatatggcct gaacatatac tccagattgt ctctgtcttc caaggtacaa
     5461 tattttcttt ttaaataaaa tgtacatgcc tggtaaagac ggtcatggag gagtgccaga
     5521 actcctgcat tgatagcatt tgttctggca cgttctgcct ttaactgttt ggataattta
     5581 tgatatgctc cagaaagcaa tacagagatg atcccaaaca cccatgaaac ccaatgcaca
     5641 gatatgtact gcatgattga ttccatgact tattcctccg tgatcagatc ttcgcactcc
     5701 agatcgatca agacctgctt tacctgtggc ttgatctttt ctggtacctg tgcgtaggtt
     5761 ttctttccct taacaataag ggttgcataa atgattgcca tagtctccac ctccttcctc
     5821 agtaataaaa agagcagcag cctaagcatt taataatgcc tcaacttctg ctctgatctt
     5881 cacaggcaca tcttcaatat taattttccc tttgcggatc agatctgcat atactcttgc
     5941 cattatgcct cacctccttc ataaagctct acaattgcca gctgggtatt tgtcacttct
     6001 tcctctaatg ctgcatattt ttcacgcaag tccggaagac gaaactctgc aatcagaacg
     6061 gtacctgtta tatctttata ctcatacact gcattgccct ctgaatcagt acctgtctgg
     6121 tttttctcaa ctctaattac ataatccttc tgtctggtca tgcgaccagc atataccaaa
     6181 tctgtcctgg atgccattgt gtccccattt tcatctaaaa gcaaaataga tttaacgcct
     6241 gacatcacag tttcaacttc ttcaaaggtc tgctctcctg gctgaaagat aatacatcct
     6301 ccatcctgct gcagctgata tccgtcagct actaattcat aactctgagt tccgattttg
     6361 atagattcca taatgttcta tctcctttct gatttgattt tgtgtataat aaaaggccct
     6421 tttaggcctt ggattgttgt ttctatgaaa ttggcttagt tttatagtaa tttagaaact
     6481 cttggaacaa acaaactcaa cacagataat catatttcca aggtagtctt gaatgctaat
     6541 aataatggag taacgtttac gattgatggc gttccttata cttcaccaat aatggttgcg
     6601 ggaaagagca ttactagaat ggaaattgat acaacattat ctaaattaac ccttgttttc
     6661 ttttatgatg gacaaatcat aaaacggtat gtatccttgt cagcatctta atttatcagt
     6721 tgcagagact tgtattccaa tagcaaacaa tctttttgta gattaagccg taccaaaaat
     6781 tatcatagtt acccattttt ttccggaaaa tgtagaacca gctgttgcaa tttttaaatt
     6841 atttccgtct tttactgctg taaaattgta aacaccgcta tcttcaactt ttgtttgaca
     6901 tataccgcta gcatatgtag gaatgcttcc aaaaatagat tgaagtgtct tagttgctac
     6961 tccatttgta aaggtaaact cttcgataat taaaacaatt gttgtagttt taacttttat
     7021 tttaaaatat cccgaatcta aattactatt ttgtttagca atcgcagtca ccacatccgc
     7081 tgcactgtct ggcaatttcg atacatcacc gatcttctga tttacagaat acaacgctgc
     7141 catactggca atcttatccg gatcattaac gatctggctt actactgctg ctgcaatggc
     7201 ttcaatggca tcactgtttc cttttattcc ttcctccata tgtgtcagtc tttccgctga
     7261 taatggcgta ctggtatcag gcttattttt ccaaagctgt tttacataag ccgtaaagct
     7321 gcttaatttc atgtttctat tcctccttca gttggataaa tatcttttga tggatacagg
     7381 tctgtatctg gatgcagggt aaacagatcc tctccaggat aaagcgtggt tgccggatat
     7441 aaggttgtct gtggacacag ggtataaatt tcaacctcga tctttatctt cccgcctgca
     7501 gtcaccgggt ttggcgtgat cgatactgat tggatataaa tactgtctgc cattattcag
     7561 ccaccacctc tatacattcc atcaaaatct cttctgctac tttataggtg aaataaaggc
     7621 gataggtcac acgtttttgc ggagctatca tggcacggat cacatcccca ttgatctcac
     7681 attcaccctg ggcttcggtt tcatagttgc ctttaagttc ccaggtggca tcccggatcg
     7741 tgaagggagc ctgatctctt gcgtgtatcc gaagtccaat gcttttattt tcacctacat
     7801 agaaatggac ccgttgcatc tttttcctcc ttccactaac agttccgtat aaatctgcgg
     7861 tgctacaagt tcagcctccc attccgtttc aagaagctgt ccatagtaag gaaccggctc
     7921 cacatgagca cacagcaatg ctgtattgac tacaaacaac atctttgcta tatatgcctg
     7981 attaccagcc tcatcctctg ccaggatctc caccacatat tccccgtccg tatcaaaggg
     8041 gaccggtaca ttccaccagt ccccttctga tcggttcagc tcgacttcac agccgtctac
     8101 acggccaaaa acacgtttga ccattatagc ctcctattag tcggtaacct ccacgctgat
     8161 cacataggtc ttgccacagt ctactgggtt tggattgatc gttacagact tgatcacagg
     8221 cgctgttgta tctactgtta cggttctggt aacggttgta gtcttacctg cagagtcttt
     8281 cgcaacaact gtaatggtat ttgtaccagt aaccagtgtc agtgccttac taaaggtacc
     8341 atcagatccg actgttactg tttccgcagt accactgttc agcttgactg ttactgtaac
     8401 agggctggaa gttgcgtcat tggtagtacc ggtaacagta accgcagtct tgttggtaac
     8461 cagtttatct gccggagctg tgatagaaag tgtaggtggt acagtatcga tcttgaatgt
     8521 aacggtcttc tgtgtagccg catttccatc aaagtcagat gcatcgatct taattgtatg
     8581 gctgccatct gccagagctg tagttggagt ataagtacac tcatatccac ctgtaacagc
     8641 tgttttagta atgctgtcac ctgtaacctt gctgccactg tcaatggtaa taccaatagt
     8701 tgcaggatta acaccagaat ccgcatctgt tactttccac ttaatagttg gcttattgtt
     8761 ggtgatataa gatcctgctg ttggactggt aatggtaata gccggtgcaa ctttctcttt
     8821 tacttttaat ttcagactga ctcccaaggt cgtatctgaa tccgtctttg tcgttgtatt
     8881 tccagccaca tccgttgcct tgaccgttac cggataataa tgtccgtcat tgatggtata
     8941 actggactta gatggagctg tgatcgtagc ttcatatttt cctgtgctgg aatttaatgt
     9001 taatgtagtt gttacgccat taatgatggc ctgtactgtt tttactgcca ttattttatc
     9061 tccttctttt tatctgtttt ttctaaccca cgtacaacaa ccgctgcatt actgagcagc
     9121 gccgcctgat ccgggccggt tacaataaga tgatcaaaaa tatagagcaa cgttgcccgc
     9181 tgctcctctg ataagttata catgtgcttc ctttctgatc aggcttttca gcctgtcaat
     9241 ttcctcctgc tggctctgca tagcagctac cagcagggga atgtaattgg tatacgggat
     9301 ggtaaaatat cttccactat gtccatacag aggaaggttt aacccctgtt ttctgcatat
     9361 cctgcggaca tcctgagcaa taaaacctat accttcatgg tctgtccgtt tcatattgta
     9421 ggataccggc tggagctgtt tgatgatctg aagtgctgtt cctgtatcta ctttatggat
     9481 attttccttt aatcttcggt ctgagtactc atctattgtc ccaatgatcg tattggctct
     9541 gagcttgtca cacccaatgg tattggcgcc tatatccgcc atggacgcat aacaatctac
     9601 agaaaaagta tccctgcatg taaatctgga tgcaagtacg gtttcaccat aaatggtagc
     9661 acctgtagca gtggtaccga tctctatatt ttttgcaagg actttaccat tctgatctac
     9721 cgtaaactga ccatcaccaa cattgatact tcccttgaac agaccttcca ctacggtcag
     9781 tttcccgtct gcagtcaagg aagaataggt ggacgtccag ctaaagcgat tgcctttaat
     9841 atcaatgccg ccactttcaa tggataattg agccgatact tctccctttt taaccatcag
     9901 ttttatgttc tctgccgtca tcgtgatctg agaagataat tctccttctg cagcagacgc
     9961 tcgttttact tctgcggcaa tctgttctgc agtgatcgtt agttttgcct ctgtacgttt
    10021 ttctaggtcc accaggtttg ctgatacttc atctacattt ttctttaaat atgcagcctt
    10081 gccttttaag cggataatgt ccgcgttggt cccctgctct tcctttcgta tttcttcacc
    10141 tgttgcgctg taggtatcct gtaaggcatg gatccctttc atagtcctct taatgatgta
    10201 agaaacaatt gtcccttctt ccgacttgat caagccggca tctcctagtt ctaaatatgg
    10261 caagcctttt agcagattac attcgtaagg aacataaggc cgactgctca ccattccata
    10321 aattcccgct gcgatctgct gtaattcatc tgcgcccttg ccgtatagca ggaaattacc
    10381 ttctactacc agacaattac cggttccata gatcgcacca atatcctctt cttcctggcg
    10441 gatttgtact gcttcaatgg cttttaccgt gtaatcttca aaaacacagg atttatacag
    10501 attttgtgaa attgtttcat catagacctg ctcattcatt tcacctggaa caaggggata
    10561 taattcatct gacggataca gattttcagc tggtgccaaa atataattcg gttgcaatgc
    10621 aatatgctgt aaaatccctt cccggtcaaa atggccaaac acaccattca tctgttcaca
    10681 tgcaatcaaa gtatcccgac cagacaattc ttctacactc attgtctttt caatcaaaat
    10741 actgtcattt ggaagataag atggaacctt ttccgtcaca ccaacgtact gacataaact
    10801 ggatctgaat tctttcaatg tcattggaaa cgaaagattg ttataccact gtgccacatt
    10861 tacatcaaac agagacatct gatccagacc tacaatatcc agatattttc tatctgtttc
    10921 tttttctgcg gatttaatga tatatactcc taaaggcatc cgatatccat tgggatatat
    10981 gtcatcacct ggaaacaggc ctgcttccgg ccatattccc tccaggatct gataaacttc
    11041 cattctggct tcttttacaa cttccgtaac attaagaagt gtaattttaa actgtgatgc
    11101 ttcgcagctt ccaaattcca gattttcatt actggacaat gcctcatcca gttccattgt
    11161 ttccgctttt atgttgctgc ttgaaagacg caggtaaggt tcatcttctc ctttgtgata
    11221 aaagtccagg atcagctgtt tatctgtact gtctgcccga tacaattctt ttaatcgttc
    11281 atccacattt aacataagct gcctccctaa tattctgtca gctggatatc aaatggttca
    11341 tagaccatat ccagttttct ctcattcaca tgggaaatcg tatattcaat atctgcgata
    11401 taaaatactc ctgacttata atccatttcc tcatcgttcc aatacgtgac agataccttt
    11461 ctttcccgct ctcctcctga cacgattccc gcatctatga tcgctttaac gactatcttt
    11521 tcacccagat acagtttccg gatcggtact gtcaggctcg tcttataatt cggcgatgtc
    11581 tgacggtgga gcaatgccgt tgcatcacgg gatgcatcca gttccagtct ctgattaggt
    11641 gtacttttat atttctccag atatttgttc ggaaggacgg tatttccaaa ttttataagc
    11701 catcctttaa aagccatagt ccttcctcct tacacaagca gcggattttt tcctgtctgc
    11761 tttcttgcca accggttacg ttttaccaca ttttcgtaca ctacctttcc atccagattg
    11821 atcatcagct gaatgtctcc acccagtcca gcacctgcct ctttcaaggc ttccagcaat
    11881 gcctgcttga tcgtagacaa tggagagacc acttctgctt cccgattgtt atctccaagg
    11941 atcgccgcaa attctccggc ctgtcttgga acaacggttc ctgtagccag tctaggaagt
    12001 ttcattgatg agacattaaa tccaaaatgc tgaccaccag caaggggcac ccagtccggt
    12061 acatcaaaac tgataccatt taaaccttcc acaatatgat tgatcgcaac ttctactgtg
    12121 gaaatgatcc cgttaaaaac actcttaaat atatctttta taccatttaa ggccttttcc
    12181 atatctccgg taaatacgcc ctgtataaac tccaggatgc cgtttaaagc gtctgttaca
    12241 ccttgggctg cagtgcttac tgctgcaaga agcgcagtaa aaaatcctcc aacattttca
    12301 atggctgcac caataagcgg ggcaaaactc tgaacgatcc agtttaaaag cggctgcagt
    12361 acctggttcc ataagacggt tagggtatct gcaacgtttc cgaataattc tacaaaactt
    12421 ttaataaatg gctgcaaata ttggcttttg acttctgtaa atttatctgc cactttctga
    12481 agtgccggca atatgtatgt ttcaaatgct tccagagcgc ttttatggat ttctgtaagt
    12541 ccctctgtaa atgcgtcaaa cataggtgct acatgctcat catatgtctg gtttatctga
    12601 tcaaacgcat ctgtaaacag atctttaaga tctcccatta tctgagcagc aactcccaga
    12661 agcccgtcaa atgcctgttt aaaaccggcc gtattatttg taagaggcgt cagcagggcg
    12721 tttaaaagat cccgcccaag cttgacaaac acttctgtca aacccatgaa ggagttggaa
    12781 aaaaagccga tcagatttgc agtaaatgtc tgcccgtttt catctgcaaa gacactgaat
    12841 acttctgcga aagctgcgga aaaatttgcc acaatatctg ctatgtcccc ggttatatca
    12901 aacatgtcta tgatatattg cttgatccgt tcctgagctt cttccagata ctttgcagtg
    12961 ccaccgacca ggtttgctgc cagcgtaata ccaactgaag caacagcccc tgcaatcacg
    13021 cctaaatcat agaccatttt atttccccag gtacttgctg cagcctggac tcgcggatct
    13081 gaaaaaatat cagacaggct ttccttaata ctctgaagcc ctttctggat cgtagcgaac
    13141 cgggaagtag tatctcctaa tcccacctta aatcctttgg tgaatagact agacagatca
    13201 ctccatttct tttttaatgc atccagggct tttgacagtg agctggatac tgccaccatt
    13261 tcaaacatct gggatggatc cgttccggaa ccgccgcctc cgctacctgc attattttga
    13321 cttaagacat ttaatttatc aaaagctgcc agactattct ttgcatcttt tgctgcgctt
    13381 cctgtctttt taagactttt tgcataatct tcctgggtct tcttagcctt cacaaaggtg
    13441 gatttcccag ttaatgccgc taccagttgc ccgatccagg ccagtgcctg ggctatcgca
    13501 tcaatcaatg cagtgattgc cggtaccgca acacttaaaa tgggagcaaa accggctgcc
    13561 aggctgttct ttaaatacaa taaagaagag gacagaccgg ataatgtctg gtttgcgctt
    13621 ccggaatatc ttgcaaggtt ttgaaagcct tcctttgtcg cgctcatcac tgccattacc
    13681 gcctgcatga caaaccgcat catcatcatg cgactgatcc ggcctaagag ctgcatccct
    13741 ttccctgagt ttttcgccgc tttaccagcg ccatttacag agtcccttaa tttatccgca
    13801 gatgcttttg cctttttctg tccggcatct gcatttaaaa gagatttttt atattcattc
    13861 tctgcctgga tcacctgctg cagttgaaca taggtctgat cataatctgc atctccaagt
    13921 gatattccct gctgctccat tcccagtagt ttacttctga gtgcttcctt ctgtccttca
    13981 aaagagtctg cgtcaaactg aaccgggatt ttaacaggag tggtaaggtt ctttttataa
    14041 gtgttcaatt cggactgtgc ctggtttaaa gctttatagg tactgtcata caaggcatca
    14101 ccaaagcttt ttccctggct ttccaggtct ttgagttctt tctttaaacg atcaatcttc
    14161 ccctgcaggg agtccgccgg aaaaataact gcgtctggcg ttggattagt cagttcctgc
    14221 ttataatctt ttaaagcctg cttgactttt tccaacttca gataagcctc atcatatttt
    14281 tcgtcaccaa agtacatccc ctggctttcc aggtctttga gttcttgctt caattcgctg
    14341 atcttctgtg caaattcatt ggttgccttt tccgcttcct cttctccgga tacatactta
    14401 tcaataaatt ctattgccgc aggatcataa ccatatgctg aaggatcgtc tacctttacc
    14461 cgtctggcag gtgtcggctc ctgttcctgg tattccccac gatctacttt gatggcatcc
    14521 atctgttcct gcaggctttt ggtctgtctt tctgcctgtt tagctgactc tgttacctga
    14581 tcaattccct cagctgcctt ggaagactgg gctgctgctg tttctgccgc ctggcccaca
    14641 ttgttaaatg cactggaaat attgtcagaa agcctgtcaa ctgctttagt gagtctgtca
    14701 aaagcttttt ccagtgttcc ggttcctttt tccagtccag aggtatcaat ttttgtatca
    14761 aatttcagac tgccatcagc cgccatatcc tcacctcttt tccaggcata aaaataagac
    14821 gctgtcacgc gcctcagcct aataacttat tccaatagtc aatttcttcc tgttcttctt
    14881 tcgtataccg tttcttaagg tcacagattt ttttattgtt tctgcgaaac tctctttccc
    14941 atttttccag gtgcttacct tttgccattt tctgccggat ccccagtacg gtggaaaaga
    15001 taccttcccg gatttccatg aaatatccca caaaggtcca ccagtgaaca tgtggaacag
    15061 atcgtacttc acatccggcc acctggttaa tagccggaaa caggatcggc tcatcctgct
    15121 cccagtccat caccttgacc ggcattatat cctcttcatc ttcctggccg cagtccagga
    15181 accagagcgc ctttttagct gactcttcat aaagttcact tggcatatcc ggccagtctt
    15241 catacaggat cttacacatg actatgtaag cttcttctac tgtaagctcc ggatcattaa
    15301 atgcctgcat gatcaccagg atatctctgt agtccgttcg gatcttccat tccttttcac
    15361 ctaccataag aatgacagga agctggccta accggatcat tttgtgtagc cgactgtgta
    15421 cttctggatc cgcttattgc ttgcctctac cccggctttc atatttttct taatgatcgg
    15481 catcagacca tccataacag actcaaacag gagctttccg ccttttactg gcgaaaatgg
    15541 ggactggcca ttaaacagta tgtcataaac gtctgcattg aaaatagcat taaaacagtc
    15601 ttttacgccc tgctctgctt tcttccactc aatacttgca ttttcgtcca tcggatctac
    15661 ggtaccatct tccagaagct ttacattacc ctgaagttca tcttttacct gttccaactt
    15721 cttcatttca tccatgcagc gttgccacat gttcagatca gaaggattga tccggatcac
    15781 acggtccgga tcctcattta ccatatattc tttataacct tcatcaaact taaggctttc
    15841 catatacagc ctccttattc actgtcgggt gtaaatttct ttgtttccag ggcaaaagtt
    15901 ccttttaccc ggttacctgt atgatgtacg ttaaatggga tctgataacc ggtggtatct
    15961 ccgccatagc ttgacacctc aatgatcgca tcttcgcagt atgccacata agatcctgct
    16021 gatccggatg ccgactccca aagatgtact tcaacaacgg tagtcttcaa gtcatccagg
    16081 gtctgacgct catcaatgat cccctgaagc cgttcaaaca taggatcacc cacaaccgca
    16141 taaaacggat ctgcagtagc ctgtggctgg tagctgtcca atgtaacgga tgtttcaccc
    16201 agaatattat tcttggtatc cacattggca ttcatttcca cgttatactc ttccaggtct
    16261 tttcctaaac ggacatacgc tgtttccttt gtgcccggaa gggcagagtc aataaagtta
    16321 cccatgaatt ttcttttgat cttctgtcct gcaacaggtg tcttatcaga catttaaaat
    16381 tcctcacttt ctactttgta ctgggcgtag atctgcagct gatacataac accctgatca
    16441 acggtatctc ccatcagccc catgctcata gcattcgctg tggttgcttt taaaaatgta
    16501 gcctgtttga cttcatcacc ctcatttact tctattcctt cctcttccgg aagctgctcc
    16561 agccaatagg ccagttccag aaggaagttg ctgtttgcca gacggctgta atcggtaaat
    16621 gactgcccca ctgcgtacat aacaaagtta tgcctgcgaa cctgattacc caggatatct
    16681 tctttcacca gggtatctcc attgcttgac aggccataat taatcggatc cggttctgta
    16741 aaatcgatat ggatatcatc accggtcaga aactcggata tcttgggata ttccgttagt
    16801 ttctgacgca tatattcaat gatcgtcata tcctgcctcc tctgctaagc actgcctgcg
    16861 ccgcctgaag gatatcatcc ttatgatctg ctttcatacg ttcaaaccat ttctttccgc
    16921 gcataggagc accagcataa gtcagctctt ttcctgttgg taccttgatc tcatttttct
    16981 tcgcccaggc acttccagtt gttggtgaca cataaaggat gccttcatac agataatgag
    17041 caaaaggacc gggagtattg atctgaccag aaccgattgt agtggcagcc accatcaggt
    17101 gtttcagctc gccggcctga cgccttggca tataatcact catatagcgc atacattcac
    17161 tgtcgattgc tgcttgcaca ggtccatttt cagcaacttt atgtctacgc cgcatttctg
    17221 ccgtagaaag catttttata actattttca tggttacacc tcctacttac aggacagctc
    17281 ataatgctgg accgcttcac taccatacaa ccgcccatcc acagttgtaa ctgtcacata
    17341 tccatgactt gctttcaacg ctgccagtga cttcgacatg acttcttggc tactgcagtc
    17401 tatttcatct tcaacaatgc cttttacggc cagatctctg ccctgggtaa atgtcagcgg
    17461 ctctgtgata ctttccaatg ggataaccaa aagtacagaa gcagcatcac gctggcctgt
    17521 ttttagaaaa gtagactgcc tcacatcttc ccaataaaca tcttcaaccg gtatccggat
    17581 gtatcgcata tccttgccgc atttgtggta taaatacaag gttacatctg cattagtaaa
    17641 catcagcaca ccccctgata gcataaaccg gtattttcca gccatttctt aacgatctgg
    17701 ttttgcttcc ttatggccgc ttctgttaat tcctgcgaag atccatagga agccgaataa
    17761 gttccgatct tttcagaagt ctttccggat gcattttttt ccgtcttttc ctgccgacag
    17821 ataacctctg ccagttcaca gcagcataat tttgcttctt ccggaacatc ttccataatc
    17881 gtcagccgtc cgaatgtata ctgatccatg atctggcttg cctgtctggc ataaaaagga
    17941 aaaccggagc tgatggccgc tttccttcca agaagatatt catttttata aaactcttca
    18001 tctgcataaa ccatcagctt ttttcctttc ttttatcacg catttttgat aagggttata
    18061 tccttcgtta ctgcagatgc aaccaccgtt acggtctcag tgatctgact gtatccggtc
    18121 tttttgatct ttgccggcta tgtaccaggt cgcaggttaa atactgcttc gcctgacgca
    18181 ttggtcttta atctggatcc atttacatct acaatagcac cttcaattgc ttccggactt
    18241 cctgcgttat cctttacagt aaaggttaca gtctgagtag ttaccggtgt tgccggttcc
    18301 agataagcaa acgggcagcc tacacggtcc tcatccattc tggttgccgg atttggaaga
    18361 gcccaaccca tacgaaatac aatacgcaga gctaccatat cctgctgagc caggttataa
    18421 acgatatcct tagtgatcgg atcctggata actccctggt caaggatctt tacagtaacg
    18481 tcctgacgaa ttgcatatac cgcctgctta aaatcaccta cgattagttg agcaatgctg
    18541 ttatcataag caccgttctg cgggaaatac ataggcgcac cgtccagtgc gtaattagtg
    18601 gatccctgca tgtcgctctt aaaaatcggt gttccgtccg ttgccttgat gcctcttaac
    18661 tttgctctca tacccatggc tgccagagcg ccagttgcca tgtaaccgtc ctcttcaact
    18721 ttggagatca cgccattctc tcccagaagc aggttgtaat aatccggagt agatccaggt
    18781 gctacgttgt tgcctgcctg acgtgccagg gtgatgatat cgttctgcca tactctcggg
    18841 cgatttacac cgaagatgat cgcagagtct actctctggc cgattgcttc atttactctt
    18901 ggagtgatct caccaaaaat atcaaactcc gcatcatcta atactgcctc tgggatcggc
    18961 acgataacag ccagctcagc tgcatccaga tatacgttat cccaagcctg gcggctggtc
    19021 tgtttcatac cagtgtcacc atccacccag tacgcagttg ggaggaaatc caatacacgg
    19081 atcctggtct ggtcactggt catgtttggc agctttcgtg ccatgctcat aaatacggac
    19141 tgcttcggtg cgtcctgaaa aatggtagat actacctgtt cgcggatgat tgcctccgca
    19201 tcagatctgc ttgtaatatg tactggcata aatcaattac ctccttattc tcttccaaaa
    19261 atacttctta aggcttcatt tgcccttgtc tttgtgtcct cagttccttt accgcccgat
    19321 ccaggagtat aagaaaccac tttgggtatc tgtgtgtcct gaaacaaata ggcattgtct
    19381 ttcttgactg cctccagagc agttttaata tccgcttcct ggttcttgct ggctttcagc
    19441 ttctctacat ccatgaatgg cattaccgct ttcagatcac ggggcttgta cccttctgca
    19501 gtagtcttta aaagatcatt aaagtcacga tccgcaatct gcttctggta ctccgcatcc
    19561 ttggctgcca gatctgcggt cagcttgatg acttttccct gcaagtcctg aacattgacg
    19621 ccctcaaagc ttttcagggt tgtctgggct gtatccaact gagtcttata gttatctctt
    19681 tctgtcttta tggcttcaat atccctgcca ttctcagcca taatgttgtc cacctgttcc
    19741 tttgacaggc ccatgtcctc taaaaatttt ctcttcattt tcttcctttc ccactacgct
    19801 ttttacgggg tcgcttccct tgtgttggta gttttacgtc attccggaca attttctgca
    19861 taaaaataac acgcatctct gcgtgcctac tgctcgatct tattacattt ggtacaccgt
    19921 cttacatagc cgccataagg accggaagcc cggctccagt gcttgcggta gtggtggcag
    19981 cattccttct ttctgaagaa cctccgcctg atccacgata taagtcccat aaaatcacct
    20041 tctttcattt gcgacatcgc aattattctt aaaaatgggt acaaaaatac caccggcctg
    20101 ctgactggtg gtattcgtaa tttttatata tgctaatcca aataactatc tggcaggcgt
    20161 cacctctcct gcatctcttt tgacccatag ggtgcgtggt tgcaacgtat tttaccacct
    20221 caaacagtca tctgggtaaa gcctttaata actatgccat ttacaatttt tgcaaattcc
    20281 tttccaatca gctttttcct taaaccgctc aggtactttg tccatcttta acacaccgtc
    20341 aactgcatct acattttcga tacaatcaat atcctcaatc atttcatcca caagaggaca
    20401 ttttacgaat ctatcatctt ccatgtctca ctatctccaa tgctttcaaa atgttgtcag
    20461 aatactcttc ccgcttaaat gcagtacgga taaatgcatc cgatgttctt acgtatgcag
    20521 ctccatcttc actgtagtac cgctcgaact gccccttcca gacggtctct gaaaatgatg
    20581 cccggcggat aaaacctttt gcttcgtcaa aagttacttc atgctgccgt tcatcgttaa
    20641 tgtgttcgct gtcaaagctg agtttttcaa aatccacttt gctcggttca agattaatct
    20701 ttccccggag acccaattcc tttaactctg attttattat agcaattctc ttccgttttt
    20761 caatagcttc cagttcttcc ttggtcggat tagcctcgcc aaccttcaac cgctccctct
    20821 gctgccgcag gcccatagcc tttgaaaatt ccacataggt cttttctgtt aatcgcttcc
    20881 tgcatctggc agcagtcagg tcatctttat cagctctggc cttttccagg agcttaatgt
    20941 cctgtttctg cttccggatc gtgcgttcca gcttccgctg atactgcagc gccgcatatg
    21001 tatcatactc tttaccatga aaggacttct tttcattctc tttcctgttc tgctccacaa
    21061 gccactcatc tgtgtattta cgttttgaaa taccaggaat aaaaggaaaa cggatgtggt
    21121 agcagttgat cccagcaaat cccagcatct ctcccaaacc acagacagta cgcatttcag
    21181 cagagctata tactttgccc tgccagcttt ggtggttcat gtatcctgtt cccatattac
    21241 gggctcctaa atgccattct acttcccagt agtccgttcc cagctccttt gcattatgct
    21301 cattcacctt atcggtcatc tgtgcaatgc cggtcataac ggctctcctg accgccacct
    21361 caatacggtc cgaccttcct gatgcataat caaccacccg aacgccactg gatgtcattt
    21421 catcgatcac atcaccgatt gcctggctgt atgtcttagc gccagtagtg atcttcatca
    21481 cggcttcatc taagctgcgt tccagatact cagacatagg agtaaatact tttttcccgt
    21541 ttcccattgg cacattaaaa ccggttgtct ttgtgatatt ctccatgggt ctaagactat
    21601 ccgttgtctg atcctttact gcctgcacca cctgttttag ccagtcatta tcctcatacg
    21661 ggatcgcatc catgcctgca gctttgtaga tctcactatt tctcacataa tcagatcttg
    21721 ctgcagtttc atagatctca tcaatatcga tccctgcagt ttttacacct tcccgcagga
    21781 tctctttgat ccgcttctta ctcatgccta tggcagtcat acgattaagc agccaatctg
    21841 tcaccggcgt gatctgggag cattcccgga tccggtttac gacctctaac atgatatcca
    21901 tttccaatgc tgtcatggta cgttctaatg gcttcggcaa cttttcaagt tcttccggcg
    21961 tcaatcagat cactcctcta cgtttgctgg ttcaggaagg ttcttctggg cctcttccag
    22021 tgtctcccca taccacttgc ttctgtactc tgccaagctc attacaccca tggcaacatc
    22081 cgcacgatcc gtctgacggt ctgcctctgc atccactaca atgctgtcat cccagtcaaa
    22141 ggaaacctga tagtcattcc ccggcggtac cagaccgtac aaggaagccc agaaattcat
    22201 ggcatatacc aaatcttcca gagcagtctg taaagcaagc tgtgtatctg acacaaaggt
    22261 ataggagcgc tgcttgctgg tcttgatctc cgttgctgtc ttatccacat tctgaggatc
    22321 cgaaagcgtt ccataagcca gacagcaggc aaactctatc aacttcaact gattattaaa
    22381 tccattgaat aatgctgtat cccggatctc cggagaaaat gtgtctataa aaggcttgtc
    22441 tgatgcaccg gtgttatact ccacgttacg gtataacctg tcctggccgc ctgggtactc
    22501 aaacttatcc aggtcatggt tatacttaag cattgaagtt gccacatgca ccgccagctg
    22561 tgttccttca tactcccagc agatattgga atagcgcctg tccccctctt tgatcagctc
    22621 taccgctcta gagtatacag atacacccaa cggacttccc gtatcattcg catttgcaag
    22681 aggcactttg aaatacccaa acagaagccg atcagcaccc tccagcactg cttccggcat
    22741 taattcagac cacctatcca ttgaatttac actgatctcg cttccaagac tgtagtcatt
    22801 ggttgcaaca aaggccctgt ttgtgatccg gatctgttct ccctgcagcg tatggacttc
    22861 taatctggta tagatcttct gtccttttcg gaactgctct gtaaacacac actgttggat
    22921 ctggccggag tcatcaaaag caagaggaaa gaaacagtct gcctgtacat actggattgc
    22981 aagacctgtt tttgtaacat acggctttaa tattaagccg cctttcgcac atccgtactc
    23041 cacatacctg cggatacttt tcagcacttt tctctgatac tggtcattca gataagcagc
    23101 ggaagaacct cctgttatct ccgacttcat ttctagggta acaagtcttg caacctcaga
    23161 ggcaatagcc gccggcagct gcgcactttt cacttttttc cggtccaccc atggggcatt
    23221 atcttcatac atagccgtcc atagctctat cctccgggcc atttcagaag tcatgcagac
    23281 atcaacctgt gtatccgagt cctgatttaa aacctgcgtg atcgcagcca gcattttaga
    23341 aaatttcatt gttatcacct ctattcatac cggataaacc ggctgatgtc ccgctcaaat
    23401 gtgtactcaa aagcatccag cgtatcaata tcactggtac cgtcatccag tcgcacatct
    23461 tccacaagac ttttcttctc gtcccacaac gccgttgtca gggcatcttc caggctttga
    23521 cactggtctt ttacatagaa aaagcggtgc tgaccaagca tccgctgcat aaaacgtatt
    23581 ctgtcattga tcgttgtctt caaagcattc tcgatccgga tccagccaag ccccgccttc
    23641 ctgaccgctg tcctcatacc tgcgatcagc gtctgctctg cactatcaca atagacaacg
    23701 gtaataaatc catacaggtt gatgatcttc aagcaaaagt ctacaaacaa ttctcccaac
    23761 ttatccggat caatactgcc atttacgctc atatggcgct cactggcaag tgccgtaata
    23821 ctttgataag ccctagaata tgcagtagca acaaatgcat gtcctgatcc tgaaccacca
    23881 aagtcaacac caatattgat ctgtaaaagg cttttaggct tctcatagat tgcatatggg
    23941 ttaatgcctc cactggatgt cgcatcacac atcaacttat acacagaacc ttccgcagct
    24001 acccacaggc cacggatgta ccggtcatac aagaccgttc ctttatattc cttgcaaagc
    24061 tcatctacaa atacccggct taaaaatgga ttatcaaaaa tctcatactt ctggcagtaa
    24121 atgtccgcat cagagtccag gaacttttta aaccagtgct gtggggcatc cgggttacag
    24181 gctccgtcaa agcaggaata aggcttatcc agacgggatt taagcatgtt aaagacatcc
    24241 tggttccagt ctacaacctc atcaccgtaa caatatttta atgaagagcc tcggatttta
    24301 gatacctggc tgactttctc agcgcccagg cagtaaacat cttccccaaa catggggcag
    24361 atgttctggg aattgatatc acctaccagc ttggttcccc agatacgctg taatggttca
    24421 atgatgtttc gctggatcgt gcctttagaa acgcccagga tcgccaccag cccttctttt
    24481 tcggctctgg cacggattct cttagggatc acataatagt ccatataggt ctttccggaa
    24541 cgtgtcgcac caaccttaat attccatcgg tggtttgcat ttcggaaaaa ctcctgctgt
    24601 ttatcagaaa atggcatact acacaacccc ttttatctcg ctaagaacct ggtccagacg
    24661 gctcagctcc tcttcattgt cggtaccctt aagtttgtcc gtctgggcct tgatctgagc
    24721 tatgcgggcc ttctgctcat cactggcaag atcccagcgc ttatgcagca actcgtcata
    24781 ctgcttaatg agactgcgaa gctccgactg tgccctggcc tgagcctgta ggaatttccc
    24841 ctgcttgtcc caagcctgct gcacctccca gcgctctccg atcacgttgc cgtccttctc
    24901 c
//
LOCUS       DBMDUA010000018        23489 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1431_length_23489_cov_2.848854, whole genome shotgun sequence.
ACCESSION   DBMDUA010000018 DBMDUA010000000
VERSION     DBMDUA010000018.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 23489)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 23489)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23489
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1431_length_23489_cov_2.848854"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="ACZPCL_02775"
     CDS             <1..500
                     /locus_tag="ACZPCL_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024945.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1161443.1"
                     /translation="LKDGNNLAATVLEENNIAYQQALTAVRYGTMLNPDKGIYFYSHY
                     YIYEMLDEYKKRYALEDMYIQKLKELKNPSEEHYDNLSLLRNYLLTERSISSTAKIMH
                     MHRNSVIYRLGKIQEALGFDLNDPDVRLRVLISFKILELISGHIEPLPCIDGQQGSES
                     FNFYE"
     gene            complement(514..1517)
                     /locus_tag="ACZPCL_02780"
                     /pseudo
     CDS             complement(514..1517)
                     /locus_tag="ACZPCL_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(1532..2338)
                     /locus_tag="ACZPCL_02785"
     CDS             complement(1532..2338)
                     /locus_tag="ACZPCL_02785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR1161444.1"
                     /translation="MNILVCIKQVPDTTEIKIDPVKHTLIREGVPSIVNPFDACALEM
                     AVRLKEKEGGKVIVISMGPAQAKNALKECLAVGADQAFLVTDRAFGGSDTLATSYILS
                     RAIAAVEAKTGEKFDLIFCGKQAIDGDTAQVGPEIAEHMDLPHVTLATAVEMADGKLH
                     VTRDTDEGHDLVEVSLPALVTVTKTTYDPRYPTIKSKMAANRAVIEEITNSDPDMVCE
                     PERLGLNGSPTKVKKTFTPVQTKDAVILNEETGSESAVKLAALLSEAKII"
     gene            complement(2385..3164)
                     /locus_tag="ACZPCL_02790"
     CDS             complement(2385..3164)
                     /locus_tag="ACZPCL_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012596.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR1161445.1"
                     /translation="MEYQNFKLEIDEQGIAVFTANRPEKMNALNDLSWAEMNQFFTWA
                     DKADEVKVIIVTGAGEKAFIAGADLNSLKTKKSTDCLGGAGQKALDLIQKCSKPVIAA
                     VNGYAFGGGCETAIACDFRVVSEDALFALPETGLGLLPGAGGTQRLARLIGLGRAQDV
                     ILLGRKIGGPEAVQIGLATKCVPKTDLMAEAKKMASKLIAKGPVAIRIAKRVVQASMS
                     SSQDVGELLEMLALSTLCSTEDKLEGVSSFLEKRTPEYKGK"
     gene            3398..4561
                     /locus_tag="ACZPCL_02795"
     CDS             3398..4561
                     /locus_tag="ACZPCL_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014564.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR1161446.1"
                     /translation="MKPLEGITVVELSTYIAAPSCGRILATQGARVIKVESPSGDVER
                     KFGPTLFCPANDEENPIYDTLNGGKDHLMLDLKKPEDMERFHKLLAKADVFVTNNRLQ
                     ALKKMRLDYDSLKERYPKLVYAILLGYGEKGPKVNLPGFDAIAMFATGGLIQDMMVDA
                     PGSYPVYLPMGFGDLVCGTALAGAIGTALYGRTQSGKGDYVSISLYGAAMWMFSVMST
                     GTQFGYKWPRGRYQGGPMGVPYKTKDGRWILPVVNEYERYWRPFCQAVGAEEIMDDPR
                     YCTKQATFDPKNREDCIRYFEDCFSKLNADEVLPKLEAIDIVVSELAHFKDNHTSEQA
                     LVNGFMSPITYPNGDQITLAMPPIKMGCVEQPHAEKAHKIGEDNEKLFKEFDL"
     gene            4612..5400
                     /locus_tag="ACZPCL_02800"
     CDS             4612..5400
                     /locus_tag="ACZPCL_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012596.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR1161447.1"
                     /translation="MDYQYILVDVKDRIATVTINRPEYSNAFARESYYEIADAMNTLG
                     ARDDVGVIVITGAGKHFSAGGDIKRFKMLVDTKTYLKAEDIEAVSAMPISIRNCPKPV
                     IAMINGVATGAGLSCALACDFRIVQPSSKMIMAFVNMGLCGDNESIYTLTRLLGPDKA
                     ELMMMTGDAYKGEQCVKIGLATVLAEDGKLAETTYALAAKLAAKSSTAHASQKRLIRK
                     YFYGDMEKFAKDEGMEIAANSRQPDFTEAVNAFIEKRMPVYNQK"
     gene            complement(5482..7902)
                     /gene="pheT"
                     /locus_tag="ACZPCL_02805"
     CDS             complement(5482..7902)
                     /gene="pheT"
                     /locus_tag="ACZPCL_02805"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR1161448.1"
                     /translation="MLVPLSWLNDYVDINVSADELEKKLFDCGFEVEEKWQVGKDISG
                     IVVGYVESCEPIPETHLHVCQVNVGGPELLQICCGADNVKAGGKFPVAMIGAQVYATA
                     KDHVTIEGVVTIKKGKLRGYESYGMLCSGTELGLNEDLYPGAGYNGLLVLPEDAEIGA
                     DVKEITGLNDWIFDVSITANRPDCQSIFGLAREVAAALGQPLKMPALDYTETEAEKTG
                     FDVTVEAPDLCPRYIAHYVYDVKLAQSPAWMRRRLALVGNNSISNIVDITNYIMRELG
                     QPLHAFDCDYLEGNAICVRRATEGEKIVTLDEKEFTLNTNNLVICDGKKPVALAGIMG
                     GLNSEIRDTTTEVMFEAAKFARDNIRKSSRALGQSSDASQRYAKGVDEYATEMAMKRA
                     LHLVEELGAGKVSKTHKNVNTGNSLEPKTFKTSIKKVNGVLGITVPDEDILRILKGLD
                     FDPEINGDELTLHFPAYREDMEDYPDVAEEVIRMYGYDHINSTFLPTAKVTIGGTNLR
                     QKTILKVKRALCSVGAFEGMHYSFFSPADLDEMEFPEDALERKAIRIMNPINEDLSLM
                     RTTLAPQMIHAMGRNQKRGIFEGRIFEIGNAFIPKSLPLTEYPEERETLCIGAFGKDE
                     SFFTLKGMAETVADVLHLTFTYERTEKPFLHPYRTAAILCEGEVVGYLGQVKYEIMKE
                     EAMREDAYVLEINMKALEKWYGKAPVFEPLPKFAEEKRDLALVMNKDITCGQVEECIY
                     QACSFVKDVTLFDVYEGQQIAEDKKSMAFTVLFTPKEEAFQADTVDGYVKKILKQLKK
                     AYEIELRS"
     gene            complement(7932..8975)
                     /gene="pheS"
                     /locus_tag="ACZPCL_02810"
     CDS             complement(7932..8975)
                     /gene="pheS"
                     /locus_tag="ACZPCL_02810"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR1161449.1"
                     /translation="MQDLKELNEVIAGIKDEICKGAETLTTSRSVYEFKKTFLDNKQG
                     RIGLLMKEMRNVPNDQKAEFGKTINGAKEWANQFFDELDQKLKEKEQQQRYESEKIDI
                     TMPPKKIRYGNLHPVTTVRNQLTDIFASMGFEVYEGTEIETDYYNFTALNTPKDHPAR
                     DMQDTFYLSPEFLLRTQTSAGQVHVMEAKKPPIKVVSPGKVFRSDDDATHSPMFSQME
                     GLVVDEGITLCDLKGMLDMFVQRIFGESTRTRLRPSYFPFTEPSVEVDVSCFQCGGKG
                     CRLCKGTGWIEVLGAGVVNKKVLENCGIDSEKYSGFAFGLGLERIAMLKYGINNIKML
                     FESDLRVLDQIDD"
     misc_binding    complement(9158..9400)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9617..10261
                     /locus_tag="ACZPCL_02815"
     CDS             9617..10261
                     /locus_tag="ACZPCL_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR1161450.1"
                     /translation="MKKQKEKHILTNQQGFTLAELLVVVAIIGILVAISVPVFAAQLE
                     RSREATDMANVRVAYAEVLTAVNSGDVNESRIVPLKQKEDGWQTPRKVMIGGITENDT
                     NNWIGNPTAKGSCKVYYDPAIGPVLQWSREFSNIHNPFNNSGVLDYIHKTYGSNVNLE
                     IDSGCTNSKMVKLIQPFIDEDKNRSSLMKTGTWAYMGSVSDTGSDHKNQDRYWF"
     gene            10313..10579
                     /locus_tag="ACZPCL_02820"
     CDS             10313..10579
                     /locus_tag="ACZPCL_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161451.1"
                     /translation="MIVSTADGKFYISNSQTTTRTSNGMTYNVIIKSTSLAGSKKQQI
                     EAYKKYIPSDPKDCFDNLNDAYAAYEKLVKSNPDYQKYENTLPY"
     gene            complement(10602..11495)
                     /locus_tag="ACZPCL_02825"
     CDS             complement(10602..11495)
                     /locus_tag="ACZPCL_02825"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1161452.1"
                     /translation="MEAKKLEILMTAVDLGSFSKASEVVGYTQSGLTHLVNSLEREIG
                     FPLIIRSHNGISLTEQGKELVPAIRQFLQANANLENQIQTITRQQTETIRIAAYASMA
                     MFWMPEILYRFRRICPKVDVDLRMVDNALEPFELLQEGKTDVIFASRQSYRNCEWIPL
                     YYELLYAILPKDYPLNGRKEFPLKEFEGQDFLMPYGRFDIDVHAAFDRAGVKAKEQAV
                     YVDDETVIRMVGKGLGISMMSEFMIRGNTDDVCCVPVTPESIRELGMGMKKETEPSDS
                     VQKLKECVLQYVKELKERKKA"
     gene            11716..13083
                     /gene="brnQ"
                     /locus_tag="ACZPCL_02830"
     CDS             11716..13083
                     /gene="brnQ"
                     /locus_tag="ACZPCL_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HIR1161453.1"
                     /translation="MNKKHLSLHQRLLVAATLFGMFFGAGNLIFPVHLGQLAGRNLLP
                     AMAGFIIAAVGIPILGVAAIGNTHSDGLQALASKVSRRYSYFFTCLLYLTIGPCFAIP
                     RCATTSFTTGIQPVLGSQISERTALLVFSAIFFALVLFFSLKPGNITLWIGKVINPLF
                     LFFLAILILSALLHPGTAVSAAQPDPSYETGAMFHALSEGYGTMDAIAGLAFGIVVVN
                     VIRQMGVDDDTAIAKEVLHSGILAGVLMALIYMLTILMGAQSLGLFAVSENGGIALSQ
                     ISSHYLGRAGLVILAVTITFACLKTSIGLVTSCSETFVQMFPDKLSYKAWAIIFTLFS
                     FIVSNMGLSTIIRYSVPVLMLIYPPAIALILLALLGKHFHHDQKVYVWVTALTWVASL
                     FDFFKTLPEQVQILFHLDKAVTLAQTFLPLFNLNLGWILPAMVGLIIGLGCKRNEKRR
                     RQALA"
     gene            complement(13132..17724)
                     /locus_tag="ACZPCL_02835"
     CDS             complement(13132..17724)
                     /locus_tag="ACZPCL_02835"
                     /inference="COORDINATES: protein motif:HMM:NF019575.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR1161454.1"
                     /translation="MERTTYMQKKRKGGFTLAEFLVVIAITTILAGVSFVAAIHYQSR
                     LRRLEMDQTAKEIFLAAQNRLSLEKAGGSLERLLAEYPDPEDPDSEDKLGLTLSSGSE
                     EKQGLYYILYQPGDEGNNDTEDIRQRLLPFGSIDETVRTDGSYAIGYNPKEGTVREVW
                     YSDKYVFQVTDLESEELAQAALSADKREKYHGEAIGYYNGESMTDPEVIPDPKLKKPT
                     LKIFNGDVLYAEVDDYMSPTAQYTMRLWVEGMSSGAKGWIDLMDFSQRQRVISIGTSG
                     VSYVILDDITWEGFRFAGLSQDLKRSDGGKEGKLIPGEDLRVYAEVSNSNGSVISEIK
                     ECNSIFQDITENKVLVSSIRHLENLDYRISGFKPEDSGEAIGLTPAEDGSNGYVVSQQ
                     EDLSWTDFRTNVVNQIHISHGDNRVVREADKLSVCYATGSGTYTSLKYTQPGCYAPVN
                     PQFVMSYEGNTHKINDLLVNVKGDTLAGGCFGNVTKDLYVKDLKLVRPDITSETSAGA
                     LVGFGINIPDRYGDPVKPLNISVENVFVQYPKITATGEKDAASDTEVDAGALVGAFSG
                     TELTISKTMAADTYRTKVAADDAEAEKDLDTAVEATYRIRSQYGVAGGLAGSVSGKLT
                     VSGCVASVYVDGYDFAGGLVGKVVDNKGDQPARIENSYVGGHTSAGKFLVHPTPAEEN
                     FDTTQGRYNVISRDTIAGGLAAILPSGSQVERTYVSASVYMHSDVYGTVPAASETDTK
                     MNKANEKQDEAAFVTVYGALNTGSSGKAASDASFRYCYSSSMVNGARSVCYSDTLKNY
                     FEENSQAISKKAFPYDKTLTSTYPMPTVVQLIQADPTTLNMIQEDQNSDSTQPKQIPK
                     FARIHIGDWMEPEKEEPEETGMQVNNGNRLWVDYVLDIPDDTGTGTNQEPLQVSFSIK
                     GQSNGNTVYYLVKFYPDLSKIMYIEEIDASKVELAYNEKNTWGWPASWTDIDKLSTKR
                     IEVTETGEKQIKVRLYLDNKAFVRSGFKYLYWQDNYIKAGDNLVIQASDKHAIPDGTD
                     PIDDKNNSYFDKLIYDETTGTYTAKVANSRHLLNLNFYDKNDFNITNVEQTDNILWTD
                     DPSVTANTEAYCKELSEAYPGVDVKIYDGWSPGNGFTNPGSFKAIDNTTIRSYDGGGH
                     TIAGLRILPPLSGNESTALFAKNDQLTMKNLNIKDPYIQGGAYGAAVLIDTAGEINDY
                     SDVRDGTYLDLENIRVYGDDIKLQGWGVGGIAVNVGVQKVTMKNVHVYGKNVLIGGAS
                     TGSNYGAGGLVGKIKAKELEVTNCSFSGYLSGKHFQHGAGGLIGNLDLSGYVKGPDKE
                     IPLIQNCYVAGRNNDYPDMTAIGDDDQFHAPYNISGYSNVGGLIGEGKGCLKIENSFS
                     MANIFSYMRGNEGSAGGLVGRYDNSSDLTVDTCYFGGKVSRVMQGAGNDPVIGYLIGR
                     TNKTGSGTSETTDATITNCVYRAWDTNDDPIGNIWGSVSGIKSVLDQFRLRATDTQND
                     NTITYDENLKNQKYPYKIWTKKPDNQTEPYRGDWLN"
     gene            complement(17802..18422)
                     /locus_tag="ACZPCL_02840"
     CDS             complement(17802..18422)
                     /locus_tag="ACZPCL_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PulJ/GspJ family protein"
                     /protein_id="HIR1161455.1"
                     /translation="MKRNGNMMRAYRKIKCHLRSQAGMTLTEMLAAVLILSMTATAIG
                     GGVAVVKEAYKKTTQKAEAQQVLATTAELITDVLSQAQEVRTGGTSGPEFFNGENGIW
                     MRLGAVPYQEADGTQEENTNKAGICKVFIADNGQETRVPLLSDGAMAKRFYTDFNVDQ
                     YSYEDGCFTVKDINVYYKADAKRSDKVPMAHLDQLTVHAVNLEGLN"
     gene            complement(18456..18830)
                     /locus_tag="ACZPCL_02845"
     CDS             complement(18456..18830)
                     /locus_tag="ACZPCL_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161456.1"
                     /translation="MKEHVDRLFGKLRSSKGETLAELLVSVLVIVLGLTMFATAMMSS
                     KKMLVKGDAVMQAYYTQRNILEGEETKNERNGRLILEQNGTGTDLGRSAGNEGAGKYR
                     IDLYSDQKGTKTGQYFRYSRKN"
     gene            complement(18838..19650)
                     /locus_tag="ACZPCL_02850"
     CDS             complement(18838..19650)
                     /locus_tag="ACZPCL_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161457.1"
                     /translation="MVVTAGKRLKSENGASLVATLLFFVLCGVAASMILAAASASAGK
                     MQQVPVTDQKRFAVESGAAFLRDELSDSKTAVKITDVRVVDSREEEPDYSEEYVYTGG
                     ETLDDDSILGSCIKQVYTSLAEEDGQNGSFSEQEGNAQNGSSPETAEQDFTFSVQLTQ
                     TDGTQTSIKDLDQLQTAAHLTMDPQYNITVDISDTQTGDDHPENRCERKLTVAAKVHV
                     DEMEEEEEHEETNENGDVISEWTITTTTRITQIYWERGMIEKTHPDTGVSGG"
     gene            complement(19654..20349)
                     /locus_tag="ACZPCL_02855"
     CDS             complement(19654..20349)
                     /locus_tag="ACZPCL_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161458.1"
                     /translation="MKSLMSYVLTKKEKAAIVVLLLLILLTGWYVGIYSPIQERIEAA
                     DTTDLEDAIAMEQVKSARIKSMQAEIQENKDADAPVVPSYNNFKEELEELNRIHGQTY
                     DFLFTFNEPEVNGTNIRRSVTVNCSAEDYDAAVEMMREILQGPYRSLIYNISMDSVSA
                     VDADFEPNIKEGRVELTFNMNYFETTEGAESLEGLQVEETQAASGGGLANSDMSNLQR
                     SDLETAAEAAFGE"
     gene            complement(20346..20981)
                     /locus_tag="ACZPCL_02860"
     CDS             complement(20346..20981)
                     /locus_tag="ACZPCL_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161459.1"
                     /translation="MMDLKKIAKMQIGGPKYPDKKRINLYQREVKKGAVAGQIVTLAA
                     FLVFLYGFSQVGIVMPMERADRAEAAYQRMEAQLEVMKQANSIMPEVKTEYAHYGNSW
                     RNDQEAVMPDRLVMLDVLKNTIFPICKTIETVRVTDDQMDLECTLERGTILKSLIKEI
                     EEDGAVRYVTASLEETEDEDLLDISKRAEDKVVRAQVTVYFYAPGESEDLS"
     gene            complement(20993..21961)
                     /gene="pilM"
                     /locus_tag="ACZPCL_02865"
     CDS             complement(20993..21961)
                     /gene="pilM"
                     /locus_tag="ACZPCL_02865"
                     /inference="COORDINATES: protein motif:HMM:NF022554.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HIR1161460.1"
                     /translation="MKNCLGIEIGHYRVKIAYMEHGELQTYVSERLETENQTDMKVYA
                     ELIKDVLKENNIRCKNAVFVLRQDDTYVKRCSVPLMTVDQLKLNLPFEFHDYIGDELE
                     QYQFDYGVIERTEDQLDLIAACCRKDLCQQFLKLAKMAKLKLIGLVPGVIGLERILEH
                     MNEGEEKKDFAVLDLGTKALRMHFFRKGIYDTTRTMEPGCEEIEQIRRGDKEQLVQLN
                     VDDDDTFWGEKGEKTLDEALSNRYQTIAVQAMRVLNFYSFNNSDNTIDSLYYCGGGAR
                     YKGLIDTLSDTVGIPVKSIGEILPELDAKKMPEWIDSPQTVGVLLV"
     gene            complement(21977..22753)
                     /locus_tag="ACZPCL_02870"
     CDS             complement(21977..22753)
                     /locus_tag="ACZPCL_02870"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR1161461.1"
                     /translation="MLHISFGITAYILFITFILGCCLGSFADCMAVRLVSGQSVLSGR
                     SHCDSCGHVLGVLDLFPVLSWLLLKGKCRYCGAKIPPESFITELIAGIGCCLVVYRYD
                     LSVMSLQGILLGLILLIVSLTDLHSWIIPDRLHVAGVLVFLATTVFMPDPLHNILRGL
                     LLGIALGGGMLVLSLLFDRLTGKESLGGGDIKLFFMTGLYLGSAWEILFYLILSCLLG
                     LGFATLRKTQKLPFGPSIAAACFIMIVYGDVMTRWYTGLF"
     gene            complement(22783..23343)
                     /locus_tag="ACZPCL_02875"
     CDS             complement(22783..23343)
                     /locus_tag="ACZPCL_02875"
                     /inference="COORDINATES: protein motif:HMM:NF019575.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR1161462.1"
                     /translation="MNMKKIKEKKGFTLAELLVVVAIVGILVAISIPVFTAQRMKAVI
                     ATNKANIRAAKAAATAEFYSNSDLLDVHNGNTLTTAAYFIYDVKEGKLSDVIKVKDKN
                     YNGAQYNGKSCNTLGKELSNKAINGEVLDQIIVFIGNAENENDKYHNNPSAIQTAPYY
                     TDDNEIGYKGGNDNPFGPDQGSSTAG"
BASE COUNT         6059 a         5899 c         4542 g         6989 t
ORIGIN      
        1 tcttgaaaga cggtaataat ttggccgcta cggtattgga agaaaataat attgcttatc
       61 agcaggcact tacagcagtc cgctacggca ccatgttaaa cccagataag ggcatttact
      121 tctattccca ttattatatt tatgaaatgc tggatgaata taagaaacgt tatgcattag
      181 aagatatgta tatccagaaa ctgaaggaac tgaaaaatcc ttcagaagag cattatgaca
      241 atctttccct tttacgcaac tatctgctca cggagcgcag tatttcttct actgcaaaga
      301 tcatgcacat gcacagaaac agcgtgatct accgtctggg aaagatccag gaagcattag
      361 gctttgactt aaatgacccg gatgtaaggc ttcgtgttct gatctccttt aagatcctgg
      421 aactgataag tggccatata gagccactgc cctgcatcga tggtcagcag ggcagtgagt
      481 cttttaactt ttatgaataa tagagagtat ggattatgct tttaatttcc tgatctctgc
      541 tgtcagtgtt ggcagtacat caaagagatt tcctacaata ccgtagtcag cagctttgaa
      601 gataggtgct tccggtcttt attgatagca acgatgcagt cagatccgga catacctgct
      661 aaatgctgga tggcacctga gataccacat gcaatgtaaa gctttggtcc tacagtctta
      721 cctgtctggc ctacctgatg tgcatgtccg atccatccgg catcaactgc tgcacgggat
      781 gcgcctacgg ctccgcctaa tacttctgcc aactcttcgc aaagcttaaa gttttcagga
      841 ccgcccattc cgcggccgcc tgctacgatc acttctgcat cttccagatt taccacacca
      901 gctgctgctt ccctgatgga ttctaacagc ttagtacgga tcttttcagc tggaacagtc
      961 acttcttctg taatgacttc cacttctttt ccctctaccg gtgtcttctt aaatacgcct
     1021 gggcgtacag taccgatctg aggtcttgta ttcgggcaca tgatcactgc catcaagttt
     1081 ccgccaaatg ccggtcttgt ccatgctacg tttccgcttt ctgcatcata atccagactg
     1141 gtacagtcag ctgtcagacc tgtacgcaga cggcaggaca gtcttggagc aaaatcacgg
     1201 ccattgttgg tagcaccgat caggatggca cttggctgat atttttcaac taaactggtc
     1261 attgcatagg tatatgcgtc aaagctgtat ttctcatatt cgccaccgga tacactgatg
     1321 atctggtcag gtccatatcc ctttatctgc tcaattgctt ccgcattgtc cttgccaaat
     1381 acaacggcaa ctaacttctc accggatttt tccgctaaca tacggcccgg atttaacagc
     1441 tccagaccta cactttttgc ttttccttca tctgtttcaa tgaataccca gatattttta
     1501 tttgtaaact cgctcatagt cctcacacct ctcagataat cttggcttcg ctcagtaaag
     1561 cagccagttt tacagcagat tcactgccgg tctcctcatt taagatcacc gcatcctttg
     1621 tctggacagg tgtaaatgtc tttttaacct tggtaggaga accatttaag cccagtctct
     1681 ccggctcgca taccatatcc ggatcactgt tggtaatctc ttcgatcact gctctgtttg
     1741 cagccatttt actcttaatg gttggataac gtggatcata agtggtcttg gtcactgtaa
     1801 ccagtgcagg taaagaaacc tctaccaaat catgtccttc gtctgtatct cttgtaacat
     1861 gaagctttcc atctgccatt tctactgctg tagctaaggt tacatgaggc agatccatgt
     1921 gctctgcgat ctcaggtcca acctgtgctg tatcaccgtc aatagcctgt tttccacaga
     1981 agatcagatc aaatttctca cctgtcttag cttctactgc tgcaatagcc ctgcttaaaa
     2041 tatagctggt agccagtgta tcagaaccgc caaatgctct gtcagttact aaaaatgcct
     2101 gatctgcgcc tactgccaga cactccttta atgcattctt tgcctgagcc gggcccatgc
     2161 tgataacgat gacttttccg ccttcttttt cctttaaacg tactgccatt tccagtgcgc
     2221 acgcgtcaaa aggattaacg atacttggaa cgccttcacg gatcaatgtg tgcttaacag
     2281 gatcgatctt gatctctgtt gtatccggca cctgcttaat acaaaccaaa atattcatac
     2341 tttccacctc tgttttataa gataatgatg tttattaact tgctttattt gcccttatac
     2401 tcaggagttc tcttctctaa aaatgaactt acgccttcta atttatcttc tgtactgcac
     2461 agagtgctga gagctaacat ttcaagaagt tctcccacat cctggctgga agacatggat
     2521 gcctgtacca cacgtttcgc aatgcggatg gcaaccggtc cctttgcgat cagcttagaa
     2581 gccattttct tagcttctgc catcagatct gtctttggca cacacttagt ggcaagtccg
     2641 atctgtactg cttccggtcc accgatcttg cgtcctaaaa ggatcacatc ctgggctctt
     2701 cccagtccga tcagtcttgc aagtctctgg gttccgccgg ctcccggtaa aagtcccaat
     2761 cctgtttcag gaagtgcaaa taatgcatct tctgatacaa cacggaaatc gcaggcaatc
     2821 gctgtttcac agccgccgcc aaatgcatat ccatttactg ccgcgatcac tggtttggag
     2881 catttctgga tcagatccag tgctttctgt cctgcgccgc ctaagcagtc tgtagatttc
     2941 tttgttttca ggctattaag atctgcacct gctataaatg ccttctctcc tgcgcctgtt
     3001 acgataatta cctttacttc atcggcctta tccgcccatg tgaaaaactg gttcatctct
     3061 gcccatgaca gatcatttaa agcgttcatc ttctccggcc ggttggcagt aaacactgcg
     3121 ataccctgct cgtcgatctc cagcttaaaa ttctggtatt ccataaaata tgaaacctcc
     3181 cttcatatct gctgttataa tattaactat ttttcggttc tatttcatct tcatattttc
     3241 caaaaataaa tttcttcccg cagtcctgat ttgtactttt taaccttaat ttcccaatac
     3301 cgttttgtta aaatataaac tatcaaagat gggattatta tatatctaca gccttccggc
     3361 tgtagatcca taaaaaacag aggaggtatt tatcattatg aaaccattgg aagggattac
     3421 cgtagtagag ctttctactt atattgcagc cccctcatgc ggacgtattt tagcaacaca
     3481 gggcgcacgg gtcatcaaag tagagtcacc atcaggggat gtggaacgta aattcggtcc
     3541 tactttattc tgtcctgcaa acgatgagga aaaccccatc tatgacacct taaacggcgg
     3601 aaaagatcac ctgatgctgg atcttaagaa accggaagat atggaacgct tccataaatt
     3661 attagcaaaa gcggatgtat ttgttaccaa caaccgtctt caggccttaa agaaaatgag
     3721 actggactac gattccttaa aagagcgcta tccaaaactg gtctatgcca tcctgttagg
     3781 ctatggcgaa aagggtccaa aagtcaactt accaggcttt gatgccattg ccatgtttgc
     3841 aactggtggt ctgatccagg atatgatggt agatgctcct ggaagctatc ctgtatacct
     3901 gccaatgggc tttggtgacc tggtatgcgg aactgcttta gcaggcgcta tcggaactgc
     3961 attatatgga aggactcaga gcgggaaagg tgattatgta tccatttccc tgtacggcgc
     4021 agctatgtgg atgttcagcg tcatgtctac cggtacccag tttggctaca agtggccaag
     4081 aggacgttat cagggcggac ctatgggcgt tccttataag acaaaagatg gccgctggat
     4141 ccttcctgtt gtcaatgaat atgaacgcta ctggagacca ttctgccagg cagttggcgc
     4201 cgaggaaatc atggacgatc caagatactg caccaagcag gcaacctttg atccgaagaa
     4261 ccgtgaagac tgcatccgct attttgaaga ctgcttctct aagttaaatg cagatgaagt
     4321 tcttccaaaa ctggaagcca tcgacatcgt tgtcagcgaa ctggctcatt ttaaggataa
     4381 ccacactagt gaacaggctc ttgtaaatgg ctttatgagt cctatcacct acccaaacgg
     4441 cgaccagatc acactggcaa tgccgccgat caaaatgggt tgcgtagaac agccgcatgc
     4501 tgaaaaagca cacaagattg gtgaagacaa tgaaaagctc tttaaagagt tcgatttata
     4561 atattttcgt atagcatttt cgcataatca ttttatggaa aggaaactat catggattat
     4621 cagtacatat tagtagacgt aaaagaccgt atcgctaccg ttaccatcaa ccgaccggaa
     4681 tacagcaacg catttgcaag agaatcctat tatgagattg cagatgccat gaatacctta
     4741 ggtgcaagag acgatgtagg cgttatcgtt atcacaggcg caggaaaaca tttctctgca
     4801 ggtggcgata tcaaacgttt taaaatgtta gtagatacaa agacctactt aaaagcagaa
     4861 gatatcgaag cagtcagcgc tatgccaata tccatccgca actgcccgaa gcctgtgatc
     4921 gctatgataa acggtgtcgc aaccggcgcc ggattaagct gcgcccttgc ctgcgatttc
     4981 cgtatcgtac aaccatccag taaaatgatc atggctttcg taaatatggg actttgcggt
     5041 gataatgaaa gcatctacac tctcacacgt ttattagggc ctgacaaagc agaactgatg
     5101 atgatgaccg gcgatgccta taaaggcgaa cagtgtgtaa agatcggcct tgcaactgtt
     5161 ttggcagaag atggaaaact ggcagagacc acttatgcac tggccgcaaa actggctgca
     5221 aaatcatcca ctgcccatgc cagccagaaa cgcctgatcc gcaagtactt ctacggtgac
     5281 atggaaaaat ttgcaaaaga cgaaggtatg gaaatagctg ccaattcccg ccagcctgat
     5341 ttcacagaag ctgttaatgc ttttattgaa aagagaatgc ctgtttacaa tcagaaataa
     5401 atcaagatat caaaaaagcg ccccctgcat ttgcaggagg cgttttttgc tacgcagccg
     5461 cttgtttttt ggctacgctc attaacttct caactcaatc tcataagcct tctttaactg
     5521 ctttaagatc ttctttacat atccatctac tgtatcagcc tggaatgctt cttcctttgg
     5581 tgtgaacagg acggtgaatg ccatggactt cttgtcctct gcgatctgct ggccttcgta
     5641 tacgtcaaag agtgttacgt ccttcacaaa ggagcaagcc tggtagatgc actcttctac
     5701 ctgtccgcag gtgatgtcct tgttcattac cagagccagg tcacgcttct cttctgcgaa
     5761 ttttggaagt ggttcaaata caggtgcctt gccgtaccat ttctccagag ccttcatatt
     5821 gatctccaga acataggcat cttcacgcat tgcctcttcc ttcatgatct cgtacttaac
     5881 ctgtcccaga taaccaacta cttcgccttc gcaaaggata gcagcagttc tgtatgggtg
     5941 caggaatggc ttttcggttc tttcataagt aaaggtaaga tgaagcacat cagctactgt
     6001 ctctgccata ccctttaagg tgaagaagga ctcatcctta ccaaaagcac cgatacacag
     6061 tgtctcacgc tcttccggat actcggtcag aggcagggac tttggaataa atgcatttcc
     6121 gatctcgaaa atacgtccct caaagatacc tctcttctgg ttgcggccca ttgcatggat
     6181 catctgagga gccagagtgg ttctcatcaa ggaaagatcc tcgttgatcg ggttcatgat
     6241 gcggattgcc ttgcgctcaa gggcatcttc cgggaattcc atttcatcta aatcagccgg
     6301 tgagaagaag gagtaatgca ttccctcaaa tgcgcctaca ctgcagagag ctcttttaac
     6361 ctttaaaatg gtcttctgac gcagatttgt tccaccgatg gttacctttg cagttggaag
     6421 gaaggtagaa ttaatatggt cataaccata catacggatc acttcctctg caacatccgg
     6481 gtaatcttcc atatcttcac ggtatgcagg gaaatgtaag gtcagttcgt ctccgttgat
     6541 ctcaggatca aagtccagac ctttcaggat gcgaaggata tcttcgtccg gaacagtgat
     6601 acctaataca ccatttacct tcttgatgct ggtcttgaaa gtctttggct ccaaggaatt
     6661 tcctgtattt acgttcttgt gggtcttaga aacctttcct gctcccagct cttctaccag
     6721 gtgcagagca cgcttcatag ccatttctgt tgcatactcg tcaacgccct ttgcatatct
     6781 ctggcttgcg tcagaagact gtcctaaggc acgggagctc ttgcggatgt tgtcacgggc
     6841 aaacttagct gcctcaaaca ttacctctgt agtagtatcg cggatctcag agtttaaacc
     6901 acccatgata cctgcaagtg caactggctt cttgccgtca cagatcacca ggttatttgt
     6961 atttaaggta aattccttct catccagagt aacgatcttt tcgccctctg ttgcacgtct
     7021 tacgcagatg gcattgcctt ccagataatc acagtcaaat gcgtgcagcg gctgtcctaa
     7081 ctcacgcata atataattgg tgatatctac gatattggaa atagaattat ttccaaccag
     7141 agccagacgt cttctcatcc atgcaggtga ctgagccagc tttacatcat atacatagtg
     7201 tgcaatgtaa cgtgggcaca ggtccggtgc ctctacagtt acatcaaatc ccgtcttttc
     7261 tgcttctgtc tcagtataat ctaaagcagg catcttaagt ggctgtccta aagctgcagc
     7321 tacttctctt gccagaccaa agatactctg gcagtccgga cggtttgcag taatggaaac
     7381 atcaaagatc cagtcattta aaccggtgat ctcctttaca tctgcaccga tctcagcatc
     7441 ctccggcagt accagaaggc cgttgtagcc ggcgcctgga tacaggtctt cgtttaagcc
     7501 aagctctgtt ccggagcaaa gcataccata ggactcatag cctcttaatt tgcccttctt
     7561 aatcgtaact acgccttcaa tggtaacatg gtcttttgca gtagcgtata cctgggcacc
     7621 gatcattgct acagggaact tgccgcctgc ttttacattg tcagcaccac agcagatctg
     7681 taataattcc ggtccgccta cattgacctg gcatacatga aggtgggtct ccgggatcgg
     7741 ctcacaggac tcaacataac caactacgat gccgctgatg tcctttccta cctgccattt
     7801 ttcttctact tcaaatccac agtcaaacag ctttttctct aattcgtctg cggaaacatt
     7861 aatatctaca taatcattta accagcttaa cggtacaagc atttctaatc ctcctgtcct
     7921 gatcgaacct attagtcatc gatctgatct aacacacgca gatcagactc aaacagcatc
     7981 ttaatgttgt tgatgccgta ttttaacatt gcgatacgct ccagaccaag gccgaaagca
     8041 aaaccgctgt acttctcgga atcgatgccg cagttttcaa gcaccttctt atttacaaca
     8101 cctgcgccaa gaacctctat ccagccggtt cccttgcaca gtctgcatcc cttaccgccg
     8161 cactggaagc agcttacgtc aacctctaca gacggctctg taaatgggaa gtaggaagga
     8221 cggagtctgg ttctggtaga ctcgccgaag atcctctgaa caaacatgtc taacattccc
     8281 tttaaatcac agagagtaat gccttcatct acaacaaggc cttccatctg actgaacatt
     8341 ggtgaatgag tagcgtcatc atcagaacgg aataccttac ctggggaaac aaccttgatc
     8401 ggaggtttct ttgcttccat tacatgaacc tgtcctgcgg atgtctgggt acgcagtaag
     8461 aactctgggg acagatagaa tgtatcctgc atatcacggg ccggatgatc ctttggtgta
     8521 tttaatgcag taaagttgta ataatctgtc tcgatctccg ttccttcgta tacttcaaat
     8581 cccatggaag cgaagatgtc agtcagctga ttgcggaccg tagttaccgg atgaaggttt
     8641 ccataacgga tcttctttgg cggcatggtg atatcgatct tttcagactc gtaacgctgc
     8701 tgctgctctt tttcctttaa tttctggtcc agttcatcga aaaactggtt tgcccattcc
     8761 tttgcaccgt taatagtctt accaaactca gccttctggt cgtttggcac attgcgcatc
     8821 tctttcatca gaagaccgat gcggccctgc ttgttgtcta agaaggtctt tttgaattca
     8881 taaacacttc tggaagtggt cagagtttct gcacccttgc agatctcatc tttgatccca
     8941 gcgatcactt cattcagttc ttttaaatcc tgcatgattt ctctcctttt ctttcttata
     9001 tactccctgc atctttcatg taaaaaatac tgagagcaca tttattgctg gtggtttttc
     9061 ctcagttcca ttcacaactt tgtgcttcgc actctactgc ctgctatgca ggcgggttat
     9121 tcgttaatgg ctaaaaaaat ccccctgcgc atacacgcag agggacgatc atttaccgtg
     9181 gtaccaccca gtttcccgca gccagatcat ataagatctt tcctgcagac actttacggt
     9241 atgtaacgta caccagacgg cactgcctac tatttaacct tcagcaatgc agctcccgtg
     9301 ggaaattcgg atatctgtct gaacggaaaa aggctcacag ccgacgacct tttctctctg
     9361 accggaaaca gtatcttaaa aacacgatct tcgcttttgt tcttcatttt atgatttcag
     9421 gcatgtctgt ctgtataaga cattcatagt ctgtaaatta ttctaggctt tttaataaga
     9481 aaagtcaagt tttttttcat ttctggcgat cagctgtata cttaaaaaaa aagaagtgtt
     9541 aaaatagaat taatgtaaac agcgttggaa tgacataatt taccgaaata tacagaatac
     9601 ataagggaag atatttatga aaaagcaaaa agagaaacac atactgacaa atcaacaagg
     9661 gtttacatta gccgaattgc tggtggttgt tgcaattatc ggaattcttg tcgcaatttc
     9721 tgttccggtc tttgcagcac agctggaaag aagcagggaa gcaacagata tggccaatgt
     9781 ccgggttgct tatgccgaag tactaactgc cgtaaattcc ggtgatgtaa atgaaagcag
     9841 gatcgtcccc ctgaagcaga aggaagatgg ctggcaaact ccacgtaaag ttatgatcgg
     9901 aggtatcacg gaaaacgata caaataactg gatcggaaat cccaccgcta aaggttcttg
     9961 caaagtatac tatgatccgg ctattggacc cgtacttcaa tggagcaggg aattcagtaa
    10021 tatacataat ccttttaaca acagcggcgt actggattac atccataaaa catatggttc
    10081 aaacgtcaat cttgaaattg attccggctg tacaaattct aaaatggtaa aattgataca
    10141 accattcatt gatgaagata aaaacagaag cagcctgatg aaaacaggaa cctgggctta
    10201 tatgggaagc gtttctgata caggcagtga tcataaaaat caggaccgct attggttctg
    10261 aacttctgta gatataaata agaatggttt ccaggccgga gaaacagtcc ctatgatcgt
    10321 cagcacggca gatggaaaat tttatatttc caattcccag acaactacaa gaacctcaaa
    10381 cggaatgacc tataatgtga tcataaaaag tacctcttta gccggaagta aaaaacaaca
    10441 aatagaagca tacaaaaaat acattccatc tgaccctaaa gactgctttg ataatttgaa
    10501 tgatgcgtac gcagcgtatg aaaaattagt caaatccaat ccggattatc agaaatatga
    10561 aaatacactt ccatattgat atggggcttg actgtaaaaa gtcaagcctt ttttcgttcc
    10621 tttaactctt tcacatactg gagaacacat tctttcagct tctgcacact atcggaaggc
    10681 tccgtttctt tcttcattcc cattcccagt tccctgatgc tttccggtgt gaccggcaca
    10741 cagcacacat catctgtatt cccccggatc ataaattccg acatcatgga aatgccaagt
    10801 cctttcccca ccatacggat cacggtttca tcatctacat acacagcctg ctctttcgcc
    10861 tttacacctg ccctgtcaaa agcagcgtga acatcaatat caaaacgtcc ataaggcata
    10921 agaaaatctt gcccctcaaa ctcttttaaa ggaaattctt ttcgtccatt taagggataa
    10981 tccttcggca aaatggcata cagcaattca taataaaggg gaatccattc acagttacgg
    11041 taactttgtc tgctggcaaa gatcacatct gtctttccct cctgtaaaag ttcaaaaggc
    11101 tccagggcat tatccaccat ccgcaggtct acatccactt ttgggcagat cctgcgaaaa
    11161 cggtacaata tttccggcat ccagaacatg gccatactgg cataggcggc aatccggata
    11221 gtttccgtct gttgcctggt aatagtctgg atctgatttt ccagattggc atttgcctga
    11281 aggaactgcc ggatagccgg caccagctcc tttccctgtt ctgtcaggga aatgccattg
    11341 tgacttcgta tgatcagagg aaatccaatc tcccgctcca ggctgtttac cagatgggta
    11401 agtcctgact gggtatatcc aaccacttcc gatgccttag aaaaacttcc cagatctacg
    11461 gcagtcatta agatctctaa tttttttgct tccataaata cactcccatg ctttcctttc
    11521 tcctgttttt caggcatttc tgctgtcagt cacagttttc gcctgttcac agagtattac
    11581 actttgttat agttctatta taaacatgga tttcacaaac aacaagaacc aacgtaaaat
    11641 gtaaagtaat aaatgatcta tataactatt caaaactacc acttataaat atgtaaaacg
    11701 aggtgatcca cgatcatgaa taaaaaacat ctttcccttc atcagcgcct gttagtagct
    11761 gctactttat ttggtatgtt cttcggcgcc ggaaacctga tctttcctgt ccatcttgga
    11821 cagcttgcag gacggaacct gctcccggct atggccggct ttatcattgc agctgtagga
    11881 attcccattc ttggggttgc agccattgga aatacacatt ctgacgggct gcaggcactt
    11941 gcttccaaag taagcagacg ttacagttac tttttcacct gtctgcttta tctgaccatt
    12001 ggaccatgtt ttgctattcc aagatgcgct actacttcct ttacaacggg gatccagcct
    12061 gttcttggaa gccagatctc agaaagaaca gcactccttg tcttttctgc catctttttt
    12121 gccctggtcc tctttttctc tttaaaaccg ggcaatatca ccctctggat cggaaaagta
    12181 attaacccac tgtttttgtt ctttctggca attttgatcc tttctgccct gcttcatcca
    12241 ggtaccgctg tatctgcggc ccagccggat ccttcctatg aaacaggtgc tatgtttcat
    12301 gctctgtcag aaggctacgg aaccatggat gccattgcag gtcttgcttt cggtatcgta
    12361 gtagtaaatg tgatccgcca gatgggagtg gatgatgata cagccattgc aaaagaagta
    12421 cttcactccg gtatccttgc aggtgtttta atggctctga tctatatgct gaccattctt
    12481 atgggcgcac agtctttggg actgttcgct gtttctgaaa atggcggtat cgcattatcc
    12541 cagatctcca gccattatct tggaagagcc ggacttgtga tcctggctgt aactattact
    12601 tttgcctgcc tgaaaacttc catcggactg gtaaccagct gttccgaaac ctttgtgcaa
    12661 atgttcccgg ataagctttc ctacaaagca tgggctatta tttttacatt attttccttt
    12721 attgtatcca acatggggct ttccactatc atccgatact ctgtaccggt cctgatgctg
    12781 atctaccctc cggctattgc cctgatcctg ctggcactgc ttggaaagca tttccaccac
    12841 gaccagaaag tttatgtatg ggtcaccgcc cttacctggg tagcttccct gttcgacttt
    12901 ttcaagactc tcccggaaca ggttcagata ctgttccacc tggataaagc agtaacactg
    12961 gcccagactt tccttcctct ctttaatctc aatcttggat ggatccttcc ggcaatggtt
    13021 gggctgatca ttgggctggg atgtaagaga aatgaaaaaa gaagacggca ggcacttgcg
    13081 taatgcctgc catttatttc acatctctat ttaattttcc gatatcgtta attaattgag
    13141 ccagtcaccg cggtatggtt ctgtctgatt atccggcttt ttcgtccata ttttatatgg
    13201 atatttctgg tttttcagat tttcgtcgta tgtaatggta ttgtcatttt gggtgtcggt
    13261 tgcacgaaga cggaattggt ctaggactga cttaataccg cttacagaac cccatatatt
    13321 accaattgga tcatcgttgg tatcccaggc tctatacaca cagttggtaa tggttgcatc
    13381 agttgtttcg ctggtaccgc ttccggtttt gttggtgcga ccgatgagat agccaatgac
    13441 tggatcattt ccagctccct gcattactct gctgaccttt cctccaaaat aacaggtatc
    13501 aacggtcaga tctgaggaat tatcatatct gccaacaagt ccaccagcac ttccttcatt
    13561 acctctcata taactgaata tatttgccat actaaagctg ttttcaatct tcaaacaacc
    13621 ttttccttct ccgatcaaac cacctacgtt actgtaacca gaaatattat aaggggcatg
    13681 aaattgatca tcatctccga ttgcagtcat atctggataa tcattatttc ttcctgccac
    13741 atagcaattt tggatcaatg gaatttcctt atctggtcct tttacatatc cagacagatc
    13801 cagattaccg atcaagccgc ctgctccatg ctgaaaatgt tttccactga gataaccgga
    13861 aaatgaacag tttgtaacct ctaattcctt cgcctttatt tttcctacaa gaccacctgc
    13921 tccataattt gatcccgttg aggcaccacc gatcaataca ttttttccat atacatgaac
    13981 atttttcatg gtgactttct gtacgcctac atttactgca attccgccaa ctccccatcc
    14041 ttgcagtttt atatcatcac cataaacgcg gatattctcc agatccagat aagtgccatc
    14101 tctgacatca ctataatcgt taatctctcc agctgtatct atgagcactg ctgcaccata
    14161 ggcgcctccc tggatatatg gatctttgat attcagattt ttcattgtca actgatcatt
    14221 tttagcaaaa agagccgtac tttcattacc agacaatggc ggtaagatcc gcaatcccgc
    14281 tatggtatgt ccacctccat cataggaacg aatagttgta ttatcaatag ctttaaaact
    14341 tcccggattt gtaaaaccat ttcctggact ccatccatca tagatcttaa catccacacc
    14401 gggataagct tccgaaagtt ctttacaata tgcttccgtg tttgcagtaa ccgatggatc
    14461 atccgtccac aaaatattat ccgtctgctc tacatttgta atattaaaat catttttatc
    14521 atagaaattc aaattcagca gatggcggga attagcaacc tttgcagtat aagtacctgt
    14581 ggtttcatca tagatcagtt tgtcaaaata actgttattt ttatcatcta tcggatctgt
    14641 accatccggg atcgcatgct tatcacttgc ctgtattaca agattgtctc ctgctttgat
    14701 ataattatcc tgccagtata aatacttgaa ccctgaacgg acaaacgctt tattatccag
    14761 atataacctt acttttatct gtttttctcc cgtttctgtt acttcaatcc ttttcgttga
    14821 taatttatca atatctgtcc acgatgccgg ccaaccccaa gtattttttt cattatatgc
    14881 caactcaacc ttagacgcat ctatctcctc tatatacatt atcttgctta aatccggata
    14941 gaatttcaca agataataga ctgtatttcc attgctctgt cctttaatgg aaaaacttac
    15001 ctgcagcggc tcctgatttg ttcccgttcc agtatcgtct ggaatgtcaa gcacgtaatc
    15061 tacccatagt ctattaccgt tattcacttg cattcctgtc tcctccggct cttccttctc
    15121 cggttccatc cagtcgccga tgtggatgcg ggcaaacttt gggatctgtt taggctgggt
    15181 ggagtcgctg ttttggtctt cctggatcat gtttagggtg gttggatctg cctggatcag
    15241 ctggactaca gttggcatcg gataggtact ggtcagtgtt ttatcgtaag gaaatgcttt
    15301 cttggatatt gcctgagaat tttcctcaaa ataatttttc agtgtatccg aataacatac
    15361 agaccttgct ccgtttacca tggatgagct gtagcaatac cggaagctgg catctgatgc
    15421 agccttgcca ctgcttcccg tattcaatgc gccataaacc gttacaaaag cggcttcatc
    15481 ctgcttctcg ttcgccttgt tcattttagt atcggtctca gaagcagccg gtacagtgcc
    15541 ataaacatca gaatgcatat aaacggatgc tgatacatag gtacgttcca cctggctacc
    15601 acttggcagg atcgcagcca gaccacctgc aatggtatct ctggaaataa cattataacg
    15661 tccctgtgtt gtatcaaagt tttcttctgc cggagtcgga tggaccagga attttcctgc
    15721 tgatgtatga ccgcctacgt aactgttttc gatccgtgcc ggctgatctc ctttattatc
    15781 aaccactttt cctaccaggc cgcctgcaaa gtcatatcca tctacataaa cagacgcaac
    15841 acaaccggaa acagtcagtt ttccagatac agaacctgcc agtccccctg caacaccata
    15901 ctgggaccga attctgtatg ttgcttcaac tgccgtatcc agatctttct cagcttctgc
    15961 atcatccgct gctacttttg tgcggtaggt atccgctgcc atggtcttac tgatggtaag
    16021 ttctgttccg ctaaaagctc ccaccagggc tcccgcatct acttctgtgt cactggcagc
    16081 atccttctcc cctgtcgccg tgatcttcgg atactgtaca aaaacattct ctacagatat
    16141 gtttaacggt ttaacaggat caccatatct gtcaggtata tttataccaa atcccaccag
    16201 cgctcctgca ctggtttctg aggttatatc cggccgcacc agcttcaggt cttttacata
    16261 cagatctttt gtaacatttc caaagcatcc gcctgccagg gtatctccct tcacattcac
    16321 cagaagatca tttattttat gcgtatttcc ctcataagac atgacaaact gtgggtttac
    16381 cggagcatag cagccgggct gcgtatattt aagggaggta taagttccgc ttcccgttgc
    16441 ataacaaaca gaaagtttat ctgcttccct tactacccgg ttatctccat ggctaatatg
    16501 aatttgattt acaacatttg ttctgaaatc tgtccaggaa agatcctcct gctgggaaac
    16561 cacataccca ttggatccat cttctgccgg ggtcaatccg atggcttccc cactgtcctc
    16621 aggcttaaaa ccggagatcc ggtaatccag gttttccaga tgccggatac tggaaacaag
    16681 caccttattt tccgttatat cctggaaaat actgttgcac tctttgattt cactgatcac
    16741 acttccgttg ctgtttgaaa cctccgcata gacccgtaga tcttctcccg ggatcagctt
    16801 tccttccttt cctccgtctg aacgtttcag gtcctggctc agaccggcaa aacggaaccc
    16861 ctcccatgtg atatcatcta agatcacata agagactcct gaggttccaa tggatatcac
    16921 tctctgtctc tggctgaaat ccatcaggtc aatccagcct ttggcgccgc tggacatgcc
    16981 ttctacccac aatctcattg tatactgggc agtgggagac atataatcat ctacttccgc
    17041 atagagaaca tccccgttaa atatcttcaa tgttggtttc tttaacttgg gatccggaat
    17101 cacttccggg tctgtcatgg actccccgtt ataatatcca atggcttctc catgatactt
    17161 ctctctctta tctgcagata atgcagcctg ggccagttct tcactttcca ggtctgtcac
    17221 ctgaaataca tacttgtctg agtaccatac ttcccttacg gttccctctt tcgggttata
    17281 accaatcgca taacttccat ctgtacggac ggtttcatca atggaaccaa agggaagcag
    17341 acgctgtctg atatcttctg tgtcattgtt tccctcatct cccggctgat acagaatata
    17401 atacaatccc tgtttttcct ctgatcctga ggataaagta agccccagct tatcctccga
    17461 gtcaggatcc tccggatccg gatattctgc cagaagccgt tccagacttc caccggcctt
    17521 ttccaggctc aaccggttct gggctgccag aaaaatctct tttgcagtct ggtccatttc
    17581 cagacgtctt aaacggcttt gataatggat agctgcaaca aaagaaacac cggcaaggat
    17641 cgtggtgatc gctatgacca ccagaaattc cgccagtgta aaaccgcctt ttcttttttt
    17701 ctgcatatag gttgtgcgtt ccatttctct atcctccgca gaatatccag tctttcttac
    17761 tgcttcattt atgcttcatc tcagctgcag ttaaactgta actaatttaa tccttccaga
    17821 tttacagcgt gtactgtcag ctgatccagg tgcgccattg gcaccttgtc tgaccgcttt
    17881 gcatctgcct tataatatac attaatatct tttactgtaa aacagccatc ttcatagctg
    17941 tactggtcta cattaaaatc cgtataaaac cgttttgcca tagcaccgtc tgaaagaagg
    18001 ggcactctgg tttcctgtcc gttatcggca ataaaaacct tgcagatccc ggctttattc
    18061 gtattttctt cctgtgtccc gtcggcttcc tgataaggca ctgccccaag gcgcatccag
    18121 atcccgtttt ccccgttaaa aaactccggg ccactggttc cgcccgtccg cacttcctgt
    18181 gcctgggata aaacatcagt gatcagttct gccgtagtgg caaggacctg ctgggcttct
    18241 gccttctgtg tggtcttctt atatgcttct tttactacag ccacaccgcc gccaatagct
    18301 gtggcagtca tggaaaggat cagtacagcc gctaacattt ctgtcagcgt cataccggcc
    18361 tgtgatctca gatgacattt tatttttctg tatgccctca tcatattccc attccttttc
    18421 atgttcctgc tccaataaat acttcttatt acttcttaat ttttcctgct atagcggaaa
    18481 tactgtcctg tttttgtacc tttctggtca gaatacagat cgatcctgta ctttcctgcg
    18541 ccctcgtttc cggcagaacg ccccaaatcc gttcctgtac cattttgttc caggatcagc
    18601 ctgccgttgc gttcattctt tgtctcttcc ccttccagga tattcctctg ggtgtaataa
    18661 gcctgcatca cagcatcgcc ttttaccagc atctttttag aactcatcat tgccgtagca
    18721 aacatggtaa gtcccagaac gatcacaaga acagatacca gaagttccgc cagcgtctca
    18781 cctttagagg atctcagctt tccaaacagt ctgtctacat gctccttcat cctactatca
    18841 gcctcctgac acaccggtat ccggatgtgt tttttctatc attccccgct cccagtaaat
    18901 ctgggtaata cgtgtggtag tggtgatcgt ccactccgaa atcacatccc cgttctcatt
    18961 tgtttcttcg tgttcttctt cctcttccat ttcatctaca tggactttcg ctgcaacagt
    19021 cagcttccgt tcacaccggt tttccggatg gtcatccccg gtctgtgtat cagaaatatc
    19081 aactgtaata ttgtactggg gatccatagt cagatgagcc gctgtctgaa gctggtccag
    19141 atcctttata ctggtctggg ttccatctgt ctgggtaagc tggacagaaa aggtaaaatc
    19201 ctgttctgca gtttcaggag atgagccatt ctgtgcattt ccctcctgct cagagaagga
    19261 tccgttttgt ccatcttcct ccgctaaaga agtatatacc tgtttaatac agctgcctaa
    19321 aatactgtca tcatccaaag tttccccgcc agtgtaaaca tattcctcag aataatccgg
    19381 ctcttcttcc ctgctgtcta ccactcttac atctgtgatc ttcacagcgg tcttactgtc
    19441 acttaattca tcccgcaaaa aagctgcccc ggactccact gcaaaccgtt tctggtctgt
    19501 tacaggtacc tgctgcattt tgcccgcaga cgcggaagct gccgcaagga tcatagacgc
    19561 ggctacgccg cacagcacaa aaaacaggag agttgccacc agactggcgc cattttcgct
    19621 ttttaaccgt ttacctgctg ttactaccat ggtttattcc ccgaatgctg cttctgctgc
    19681 tgtttccagg tcagagcgct gcagatttga catatcagaa tttgccagac caccgccgga
    19741 agctgcctgt gtctcttcta cctgcaggcc ttccaggctt tcagcccctt ctgttgtttc
    19801 aaaataattc atattaaagg tcagttcaac acgaccctct ttgatatttg gctcaaaatc
    19861 cgcatccacc gcagaaacac tgtccatgct gatgttgtag atcaggctgc ggtaaggtcc
    19921 ctgtaagatt tccctcatca tttccacagc agcatcgtaa tcttctgccg aacagttgac
    19981 ggtaacactt cggcggatat tggttccatt tacctccggc tcattaaatg taaaaagaaa
    20041 atcataagtc tggccgtgga tccggtttaa ttcttccagt tcttctttaa aattgttata
    20101 agaaggcacc accggagcat ctgcatcctt attttcctgg atctcagcct gcatggattt
    20161 gatccttgct gactttacct gctccattgc aattgcatct tccagatctg tggtatccgc
    20221 tgcttcgatc cgttcctgaa tgggagaata aatccccaca taccagccgg tcagcaggat
    20281 cagcagcagc aaaacaacga tagctgcctt ttcttttttt gtcagaacat aactcattaa
    20341 ggatttcatg ataagtcctc actctctccc ggcgcataaa aataaacagt cacctgtgcc
    20401 cggaccactt tatcttctgc ccgtttagaa atatccagca ggtcttcatc ctctgtttct
    20461 tccagagacg ctgttacata gcgcacagca ccatcctctt cgatctcctt gatcaggctt
    20521 tttaaaatcg ttccccgttc cagggtacat tcaagatcca tctgatcatc cgtaactctg
    20581 actgtttcaa tggttttgca tataggaaaa atagtatttt tcagtacatc taacattacc
    20641 agacggtccg gcatcactgc ttcctgatca tttctccagg aatttccata gtgggcatat
    20701 tctgttttta cttctggcat aatgctgttg gcctgcttca taacttccag ctgggcttcc
    20761 atacgctgat aagcagcctc cgcacggtcc gctctttcca taggcattac aatacccacc
    20821 tgggaaaaac cgtacaggaa tactaaaaat gccgccaatg taacaatctg ccctgcaacg
    20881 gcaccttttt ttacttcacg ctgatacaga ttgatccttt ttttgtccgg atatttcgga
    20941 ccaccgatct gcattttagc gatctttttt aaatccatca cggtcttcct ccttatacaa
    21001 gtaatactcc tactgtctga ggactgtcga tccattccgg catcttcttt gcgtctaatt
    21061 ccggaagtat ttctccaata ctttttacag gaattcccac tgtatcactt aaagtatcga
    21121 tcagtccctt gtatctggca ccgccgccgc agtaataaag actgtctatg gtattatcac
    21181 tgttgttgaa actgtagaag ttaagtacac gcatggcctg taccgcaatg gtctgatacc
    21241 ggttacttaa agcttcatcc agagtttttt cacctttttc gccccagaat gtatcatcat
    21301 catctacatt taactgtacc agctgttcct tatctccccg tcggatctgc tcgatctctt
    21361 cacaaccagg ctccatggta cgggtggtat catagattcc cttgcggaag aaatgcatac
    21421 gcagggcctt agttcccaga tccaggactg caaagtcttt cttttcttca ccctcgttca
    21481 tatgctccag gatacgttcc agaccgatca caccggggac cagaccaatc agctttaatt
    21541 ttgccatttt tgcaagtttc aaaaactgct gacacaggtc ttttctgcag caggcagcga
    21601 tcaggtccag ctgatcctct gtcctctcga tcacaccata atcaaactgg tactgctcca
    21661 gctcatctcc aatataatcg tggaattcaa atggcaggtt cagctttaac tgatccactg
    21721 tcataagagg aacactgcac cgctttacat aggtatcatc ctggcgcagt acgaacactg
    21781 cgttcttgca gcgaatatta ttttctttta aaacgtcctt tataagctcc gcgtatactt
    21841 tcatatccgt ctgattttca gtctccagac gctcggaaac ataggtctgc agttcgccat
    21901 gttccatata tgcgattttt acacggtaat gtccaatttc aatacccaaa caatttttca
    21961 tatattatac tccgctttaa aatagccccg tataccaccg ggtcatcacg tctccatata
    22021 cgatcataat aaaacatgct gccgcaatag acggaccaaa gggaagtttc tgggtttttc
    22081 tgagagtagc aaaaccaagt cccaaaaggc agctcaaaat aaggtaaaat aaaatttccc
    22141 aggctgaccc aaggtacaac ccggtcataa aaaataattt aatatctcct ccgcccaggc
    22201 tttcttttcc tgtgaggcgg tcaaaaagga gggataatac aagcatgccg ccgcctaagg
    22261 caattcctaa cagcaatccc ctgaggatat tgtgtaatgg atccggcatg aaaacagttg
    22321 tagctaaaaa tacaagaaca cctgccacat gaagacggtc aggaatgatc caggagtgca
    22381 ggtctgtaag actgacgatc agcaggatca atcccagaag gatcccctgt agactcataa
    22441 cagacaggtc ataccggtat actaccagac agcagccaat ccctgctatc agctccgtga
    22501 taaaggactc cggcgggatc tttgcgccgc agtaacggca ttttcccttt aaaagcagcc
    22561 aggaaagaac agggaacaga tccagaaccc ctaaaacatg gccgcagctg tcgcagtggg
    22621 aacgaccgga aagaacagac tgaccggata ccaggcgcac cgccatacag tctgcaaagc
    22681 ttcccaggca gcagccaagg ataaatgtaa tgaaaagaat ataggctgta atgccgaaac
    22741 tgatgtgaag catattgtaa atctcctgtt tataccaata gatcaaccag cagtagatga
    22801 accctggtct ggaccaaatg gattatcgtt tccacccttg tatccaattt cattatcatc
    22861 tgtataatag ggagctgtct gaattgctga aggattgttg tgatacttat cattttcgtt
    22921 ttctgcattt cctatgaaaa caatgatctg atccaatact tccccattta tagccttatt
    22981 actaagctct tttccaaggg tgttgcagct ttttccattg tactgggctc cattgtagtt
    23041 cttatccttc accttaatta catcactcag ctttccttct tttacatcat aaataaaata
    23101 ggctgccgta gtaagggtat tgccgttatg tacatctaaa agatctgaat tgctgtaaaa
    23161 ttctgccgtt gcagcagctt ttgcggcacg gatattcgct ttatttgttg caatgaccgc
    23221 tttcatcctc tgggctgtaa aaacaggaat actgatagct actagaatac ctacaatggc
    23281 tacaactacc aaaagttctg ccagtgtaaa gccttttttc tctttgatct ttttcatatt
    23341 cataatttca ccgccttggt acaacttttt ctctccggtt tcttatacaa gaaaatggat
    23401 gggggaaacc ccatccattt tctccacgta tgtggtttct ggtgttttga aagattttca
    23461 aaaattattt tgactcagtt gttgatgat
//
LOCUS       DBMDUA010000019        22887 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1464_length_22887_cov_2.798297, whole genome shotgun sequence.
ACCESSION   DBMDUA010000019 DBMDUA010000000
VERSION     DBMDUA010000019.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 22887)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22887)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22887
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1464_length_22887_cov_2.798297"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="ACZPCL_02880"
     CDS             complement(<1..634)
                     /locus_tag="ACZPCL_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1161463.1"
                     /translation="MDLRVLRYFLTVAKEQSFTKAAKQLHITQPTLSRQLAALEAELG
                     VTLFDRGGHGITLTNEGILLKRRALEMVDLEDKIASEFKGSDGIVEGTITVGCGEFMA
                     VEIFARICKIYKEKYPMVRFAVHTGTADTVFEMMNRGLVDIGLFLEPVNTEGLDYIRI
                     QGSDHWIVGIKPDDPLAEKEYITKEDLIDLPLILPERLTVQSELANWFGKD"
     gene            757..1476
                     /locus_tag="ACZPCL_02885"
     CDS             757..1476
                     /locus_tag="ACZPCL_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008750755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR1161464.1"
                     /translation="MQNKRQVITKAFKCAFPHTIPIFAGFCFLGMTYGIYMNVSGFRF
                     WYPMLMSLTIFGGSLEFLAVSMLLAPFAPVQAFIMALMIQARHLFYGIAMLDKFKGMG
                     WKKPYLIFGMCDETFSINYTADIPADVDRSWFYFFTTLLNHFYWFSGATIGGLVGSLL
                     KFNTDGLDFVMTAMFTVIFLEQWLKEKHHISEWIGLGASIGCLLLFGADNFLIPTMIC
                     ILVALTILRKTLEQRKEVDAA"
     gene            1473..1799
                     /locus_tag="ACZPCL_02890"
     CDS             1473..1799
                     /locus_tag="ACZPCL_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter permease"
                     /protein_id="HIR1161465.1"
                     /translation="MTLSQQIITIAMCVLGTMLTRFLPFAVFSSKRPTPPYIQYLGKV
                     LPGAIFSMLVVYCLRHVSLLQGSHGLPELIAILLTVGLHLWKRQMLLSIAGGTVCYML
                     LIQFIF"
     gene            complement(1898..2665)
                     /locus_tag="ACZPCL_02895"
     CDS             complement(1898..2665)
                     /locus_tag="ACZPCL_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR1161466.1"
                     /translation="MKKVVQTAGRNALGTFAPDFAHYNDDVLFGENWNNQDIDLKTRS
                     IITVVALMASGITDSSLKFHLQNAKAHGVTQKEIAAVITHVAFYAGWPKGWAVFNLAK
                     EVWADNEGDLPYEDEAMRAHAKEMVFPIGAPNDGFAQYFSGRSFLAPVSTSQVGIFNV
                     TFEPGCRNNWHIHHAKSGGGQILVCVAGRGYYQEEGKEAVEMKPGDCINIPAEVKHWH
                     GAAPDSWFSHLAVEVPGVEGSNEWCEPVSDEEYGKLK"
     gene            complement(2703..3173)
                     /locus_tag="ACZPCL_02900"
     CDS             complement(2703..3173)
                     /locus_tag="ACZPCL_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HIR1161467.1"
                     /translation="MKKTLAAYFSASGTTRKVAKMIAEAGDCALYEITPKELYTNDDL
                     NWMNKKSRSSMEMSDKKIRPQITDTDAHIEEYDTIILGFPIWWYVAPTIVNTFLEKYD
                     FSGKKIVLFATSGGSGFGNTVKELKPSAPGAEIVEGKLLNRANKQEIEKWVKSL"
     gene            complement(3346..3939)
                     /locus_tag="ACZPCL_02905"
     CDS             complement(3346..3939)
                     /locus_tag="ACZPCL_02905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HIR1161468.1"
                     /translation="MTEWEKAQNGYLYDANYDKEIIEARTRCADLCYEFNNCRPSDTE
                     KQQKLLGQILGRMGADPVITAPFYCDYGFNVSIGKNFYTNHNVTILDCAKVTFGDNVF
                     IAPNCVFSTAGHAIDSEQRSRGLEIALPITVGDNVWIGTNVSVLLGVTIGSNTIIGAG
                     SVVNKDIPDGVIAAGNPCKVIRKITDADKKKYPVFEG"
     gene            complement(3951..4406)
                     /locus_tag="ACZPCL_02910"
     CDS             complement(3951..4406)
                     /locus_tag="ACZPCL_02910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1161469.1"
                     /translation="MKIYEVKERTELLMEQLLKVWEESVRATHQFLSEKEIEKIKEYV
                     PQALAGVQSLVIAVNGSGEPAAFMGIEDHRLEMLFLSQKERGKGLGKQLLLYGIQNYG
                     IEELTVNEQNPQAVGFYEHMGFETYKRTDMDEEGNPYPLLYMKRNDERV"
     gene            complement(4393..4935)
                     /locus_tag="ACZPCL_02915"
     CDS             complement(4393..4935)
                     /locus_tag="ACZPCL_02915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1161470.1"
                     /translation="MSFLDLLKHRRSIRQYKEEAVSKEALEAIVEAGLLSPSGRNIRP
                     WELIVVQKKETLEKMSECRVGSAKMLAGASVAIVVIADPEKTDVWTEDCSIVMSNMHN
                     MAHDLGLGSCWIQGRLRQAPDGRTTEEYLRDILGYPKEYCLEAVLSIGVPAVHPEGRK
                     LSEVPEHKVHFEMYGKYENI"
     gene            complement(4966..5940)
                     /locus_tag="ACZPCL_02920"
     CDS             complement(4966..5940)
                     /locus_tag="ACZPCL_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR1161471.1"
                     /translation="MREIHWAVLGTGVIANEMATALEKNGKKLYAVGNRTYEKAVKFG
                     EKYGIKKVYTDFNEMFTDPEVDVVYITTPHNTHIQFMKKAIENGKHILVEKSITLNSE
                     ELDEALELAKEKGVIVAEAMTIFHMPVYKELKKILESGKLGKVNLITMNFGSFKEYNM
                     ENRFFNRNLAGGAMLDIGVYALSFIRWFMDSKPDQLLSQMKAAPTGVDEQAGLLLTNK
                     EGQMATVMLSLHSKQPKRGMVSCEKGYIEIMEYPRAWEAKIVDAESGETQVIKAGVHE
                     DALAYELSDMEKAIAGDVSCMYPDYTKDVMDLMTEFRKSWHFTYPEEE"
     gene            complement(5983..7146)
                     /locus_tag="ACZPCL_02925"
     CDS             complement(5983..7146)
                     /locus_tag="ACZPCL_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1161472.1"
                     /translation="MERNTTFTEGKILQPLILFAMPVLFALFLQAMYGAVDLLVVGKF
                     ASSADVSAVSTGSQIMMTLTNLISSFAMGTTVLLGQLIGCGKKEEGGKAVGTAMVMFA
                     GIALVMTVLLVCFAPQISSIMHAPKEAFDKTAAYVRICGCGMIVIVAYNLIGCIFRGI
                     GDSKTPLFTVAIACVCNIAGDLVLCAGFHMGTAGAACATVFAQVVSVVVSFGFIRKKQ
                     LPFVFKKENVRIHKTLLKKMAGLGAPIALQDLLVSISFLIILAIVNSMGVTASAGVGV
                     AEKVCAFIMLISSAFMQSMSAFVAQNYGAGRMDRAKKALYNGIGQTKFVMLQGIAGAF
                     GVRVPVSYLMSIRPDTSLFKIGLATPMSSVVQLLLCLGFMVILNRRARKGEIR"
     gene            complement(7328..7693)
                     /locus_tag="ACZPCL_02930"
     CDS             complement(7328..7693)
                     /locus_tag="ACZPCL_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR1161473.1"
                     /translation="MIRKDNVLEVEHLGGGKGTAYVHHIVSKEELLGHGRLYAKVVLP
                     PGASVGWHQHVHDTEPYYILKGEADFIDNDKSVTKVGPGDCCIIEVGQYHSIENNSDS
                     DVEFMALIYNEPGYLNDRD"
     gene            complement(7845..8738)
                     /locus_tag="ACZPCL_02935"
     CDS             complement(7845..8738)
                     /locus_tag="ACZPCL_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1161474.1"
                     /translation="MTSNFEYYRIFYYVAKYGNLTRAASALNTSQPAVTRTIHKLEDD
                     LGCRLFIRSKMGMELTAEGQQFFEYVSAGCAQFFKGESDLKDRLSLEKGTISISATET
                     ALHCYLFQAMEEFSRKYPKVHFQILNNSTRDSIQAVRDGKVDMAVVSSLPLHMGDPLK
                     MKVLRSYREILIGGPRFEQLKGRKCSLRELSDLPWISLTSEAISRNFLNSYFLQYGLT
                     FSPDLELATTDLILPAVRHNLGIGFIPEEIAAEDLKAGVVFAIDLEEAFPERNVLMIR
                     DSEYPLSVAAKAFQKFAQERE"
     gene            8998..11391
                     /locus_tag="ACZPCL_02940"
     CDS             8998..11391
                     /locus_tag="ACZPCL_02940"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HIR1161475.1"
                     /translation="MENNTGTCLEKQPLCEEMLTKMNAYWRAANYLSAGQLYLLDNPL
                     LKKPLSLDQIKKKIVGHWGTVPGQNFVYVHCNRVIKRYDLDMILLSGPGHGGNFLIAN
                     TYLDGSYSEIYPNISQDEEGMQKMFKQFSFPCGVPSHCAPETPGSINEGGELGYSIAH
                     AFGAVFDNPDLIATVVVGDGEAETGPLATSWQSNKFLNPVTDGAVLPILHLNGYKISN
                     PTIFSRIPHDEIEDFFKGCGWKPYFVEGDDPMTMHRKMAETMDTVIEEIKAIQKHARE
                     TGDAERPKWPMIVLRTPKGWTGPKVVDGQKIEGSFRAHQVPISMENPEEHLKLLNDWL
                     RSYHPEELFDEKGRLIPELAELAPKGNARLGANPHANGGLLLKDLRLPDFRTYGIPVE
                     PGKTKAQDMIELGGYIRDIFRLNEDNKNFRIFGPDESMSNRLYRVFEAENRDWNGELK
                     DDDDKLARGGRIMDSMLSEHMCEGWLEGYLLTGRHGFFASYEAFIRIVDSMAAQHAKW
                     LKVCNQLSWRQPIASLNFILSSNVWQQDHNGYTHQDPGFLDHIANKKADVVRMYLPPD
                     TNCLLSCFDHCIRSKNYVNAIVASKHPSCQWLSMEQAVKHCTQGIGIWEWASNDCGEE
                     PDLVMACCGDTPTLETMAAVTILRDELPELKIRVVNVVDLFKLESNTKHPHGLSDAEY
                     DAIFTKDTPVIFAFHGYPTLIHELTYGRNNHNVSVHGYQEEGTITTPFDMRVQNQLDR
                     FDLVKDAIMHLPQLGNRGSFLIQKMNDKLVEHKQYIAEYGQDLEEIRNWQWHTPEDK"
     gene            complement(11630..12262)
                     /locus_tag="ACZPCL_02945"
     CDS             complement(11630..12262)
                     /locus_tag="ACZPCL_02945"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR1161476.1"
                     /translation="MTRVKLCGMMRPEDIQAVNEIKPEYIGFVFAKKSRRYVSKEQAK
                     MLKEMLDPAVKAVGVFVNEPVENVADLLNEGIVDMAQLHGVEDEAYICRLRELTGRGE
                     DTFIIKAVQVKSEADVKAGEQSGADLVLFDAGAGDGKVFNWKLLENVKRPYFLAGGLD
                     PENVRDAVERLRPFGVDVSSGIESFGVKDALKMAKFVAAVRKGSGSNAFP"
     gene            complement(12308..13096)
                     /gene="trpC"
                     /locus_tag="ACZPCL_02950"
     CDS             complement(12308..13096)
                     /gene="trpC"
                     /locus_tag="ACZPCL_02950"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR1161477.1"
                     /translation="MTILDQLADHARERVAYGKEKVAADEMRRRAFDLGREGRLGSFA
                     FEKALKKLGISFICECKKASPSKGLIAPDFPYLKIAKEYEAAGADCISVLTEPKWFLG
                     SDAYLREIAESVGIPCLRKDFTVDEYMIYEAKVLGASAVLLICSILSKEQLKEYRCIC
                     DELGLSALVEAHDEAEVEMAVEAGARMIGVNNRNLKDFSVDTENSRRLRSMIPAEVLF
                     VSESGVRDENDVRKLREIGADAVLIGEALMRAEDKKAKLAALRG"
     gene            complement(13098..14123)
                     /gene="trpD"
                     /locus_tag="ACZPCL_02955"
     CDS             complement(13098..14123)
                     /gene="trpD"
                     /locus_tag="ACZPCL_02955"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR1161478.1"
                     /translation="MIKEAIVKIVNKEDLSYEEAYTVMNEIMSGETTPTQNAAFLAAL
                     STKSARAETTDEIAGCAAAMRDHATKVETDMDLFEIVGTGGDNAGSFNISTTAALVAA
                     AGGVKVAKHGNRAASSKCGTADCLEALGVNIEQSPDLCRKLLKEVGMCFFFAQKYHTS
                     MKYVGAIRKELGFRTVFNILGPLTNPGTPTMQLLGVYDEYLVEPLARVLVSLGIKRGM
                     VVYGQDKLDEISLSAPTTICEIRDGWYRTSVITPEEFGFERCTKDDLKGGTPAENAEI
                     TRSILRGEKGHKRNAVLMNAGAALYIAGEAEDMKSGIALAAELIDSGKALDTLEKLIE
                     VSNGEAE"
     gene            complement(14239..14805)
                     /locus_tag="ACZPCL_02960"
     CDS             complement(14239..14805)
                     /locus_tag="ACZPCL_02960"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009663271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR1161479.1"
                     /translation="MILLIDNYDSFSYNLYQLIGEINPDIKVIRNDEMTVEEIRQLKP
                     ERIILSPGPGRPEDAGVIVDVAKTLGKEIPVLGVCLGHQAICAAFGATVTYAKELMHG
                     KQSEVTFDTESLLFKGCPKKAPVARYHSLAADPETMPDVLKVTAVTDDGEIMAVQHVE
                     YPIYGVQFHPESIMTPDGKGMIQNFLEH"
     gene            complement(14802..16268)
                     /gene="trpE"
                     /locus_tag="ACZPCL_02965"
     CDS             complement(14802..16268)
                     /gene="trpE"
                     /locus_tag="ACZPCL_02965"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR1161480.1"
                     /translation="MKFFPELEEVKKIAADGKYRVLPVSCEMLSDFTTPIETMKILKN
                     VSTHCYMLESAMANDKWGRYTFMGYEPKMEITCRDGEMKAGSFSVKIEDPSKFLRQIL
                     ADYKSPRFDYLPPFTGGLVGYFSYDYLGYSEPAVRCKVEDTENFKDVDLMLFDKVIAF
                     NHVRQKIILMVNMSLDDVEVGYNKAVMELKQLAELLKKGEKKQEPGGKLTGEVTPLFN
                     REQYCAMAEKAKHYIREGDIFQIVLSNRLSAPFEGSLLNTYRVLRTLNPSPYMFYFSG
                     TDVEVAGASPETLVKLEDGVLHTFPLAGTRPRGKTDEEDKALEEELLADKKELAEHNM
                     LVDLGRNDLGKISKFGSVKVEKLHSIERYSHVMHIGSTVRGEIDEKHDALDAIEAVLP
                     AGTLSGAPKIRACQLIGELENNKRGIYGGAIGYIDFTGNMDTCIAIRIAYRKNGKVFV
                     RSGAGIVADSDPKKEFEECLNKARAALKALEMAQEEEL"
     gene            complement(16687..18159)
                     /locus_tag="ACZPCL_02970"
     CDS             complement(16687..18159)
                     /locus_tag="ACZPCL_02970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR1161481.1"
                     /translation="MKKKTTYYKHYTMVTGLFILSMILGIMIFFGYVQSSVGKNSRQT
                     MMNNVSRQSQHLRTILSIHYQYLNEIAEEMAKEEELFSQINLDTLVTIHNKTDLDRVA
                     LIETDGTAHYDTGDVKNVSHRRYFNEAVNGQQTLSDPIESSVDKEVRVVLGVPVLKNG
                     EIAGILGGSYNVTALSHMMFDDLFGGNGYSLITDKDGNVITYDRVYSEQEQEVFSGNI
                     FDYFDNCQIRTAAIQDIKTDFAAGEEGIREFRFSAGRKPFYYIAYTPLGMNDWMISYI
                     VPVRAAEASYDFIRDYEIIFIIVFGMLIAAMVLFIVYKNRQEKARLLKRAQRDALTGL
                     YNKKTTQELIERSLEEGEAESCNGLLIMDVDYFKQVNDTYGHIVGDKVLKTFGHLLAR
                     QFREHDIAGRIGGDEFMVLIRNINNGSIAENRVKKLIDEVRSLEIPEMDGNGITISVG
                     LAFSPEHGTTFMELYRHADTALYQVKQGGKNGFSVYEKAN"
     gene            complement(18387..19637)
                     /locus_tag="ACZPCL_02975"
     CDS             complement(18387..19637)
                     /locus_tag="ACZPCL_02975"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR1161482.1"
                     /translation="MALAVLLVVGVSAGIYITGRKVIRTEQSSGTGNGAPGITDGEKA
                     SKPDLKKVSSKMQMIQSIIDQVFLYDEDATEVEEYIYRGMMAGLNDPYSVYYTAEDYK
                     AIQESTDGSYSGIGAMISQNKTTGLCTVVKVFEGSPALEAGMKPGDIIYKVGDTLVAG
                     ESLDVLVSNYIKGKEGTDVQITVYRAESDTYEDMTITRRKIEVPTVESKMLADNTGYV
                     AVSEFDAVTVEQFEKAIDELENQGMKQMIIDLRSKPGGMLDSAVAMVDYILPDDRKDF
                     EKGEGKTLIVYTADKNGKGDTYTAADGHEVDVPIAILVNENSASASEVFTGALKDYKK
                     AVVVGTTSYGKGIVQNLIPLGDGSAIKITTAHYYTPSGFDLHGKGITPDVEVELDEKL
                     KSQSVVTPEEDNQVQAAMEALKEN"
     gene            complement(19832..21037)
                     /locus_tag="ACZPCL_02980"
     CDS             complement(19832..21037)
                     /locus_tag="ACZPCL_02980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR1161483.1"
                     /translation="MKNRKNKFLALGLGVVLGLSCVFSSYATSADLKDAKKKVSSLQE
                     EKKKVQDTLKQLEGLKSDAAAYVKELDKKLTSLDSELSQLEKDISAKEDQIENTKAEL
                     EEAKAVEKEQYASMKLRIQYMYENGNVSFADSIMTSKNASQLLNRAEYASQIATYDRK
                     MLKEYQATREKINQKEQDLETEHSNLLALQESTEAKQASVKELMSSKQKELTSYNSQI
                     GAAQSSIDQYNADIKAQENQMKAIEAEMKRKEEEARKKAEAQKKTYNVTNLGNISFKW
                     PCPSSGRITSGFGGRKSPTKGASSNHQGIDISASTGASIVAAADGTVVIATYSYSAGN
                     YIMIDHGGGVSTVYMHCSQLLASQGQKVKKGQTIARVGSTGYSTGSHLHFGVRSGGSY
                     VNPSKYVSP"
     gene            complement(21052..21954)
                     /gene="ftsX"
                     /locus_tag="ACZPCL_02985"
     CDS             complement(21052..21954)
                     /gene="ftsX"
                     /locus_tag="ACZPCL_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIR1161484.1"
                     /translation="MSTFLYCLKQGVINICRNIWFSLASVATISACIFLFCLFFAMVA
                     NVQHGARAAEETVGISVFFDEGMDEEQIRSIGSQIQSWSEVREARYISAEEAWESFKS
                     EYFEGMEELAEGFEADNPLANSSSYEIYLKDISNQDMIVKRLEGMEGVRKVRYSSALV
                     AGFTSAGKMIGALSAVIIGVLLAVAIFLISNTISVAAAFRRRENEIMRYIGATNFMIR
                     APFIVEGLLLGLFGAVIPLAGMTILYKRGLVYLNTNYQMITGLFELLPLKQIFPVMAG
                     AAMALGVGIGAIVSFLTIQRHLRV"
     gene            complement(21992..22666)
                     /gene="ftsE"
                     /locus_tag="ACZPCL_02990"
     CDS             complement(21992..22666)
                     /gene="ftsE"
                     /locus_tag="ACZPCL_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR1161485.1"
                     /translation="MIEISHVSKSYGSGSKAIRDLSLTIDDGEFVFITGRSGSGKSTL
                     IKLLMKELDPTKGRIVVNDMDLGKMPRRYVPKYRRKLGVVFQDFRLLKDRTVFENVAF
                     AQRVIGVPSRTIRETVPQMLRLVGLSSKYKAYPGQLSGGEQQRVAIARALINKPEVLL
                     ADEPTGNLDSFNTHEIMNLLDEINQRGTTVIVVTHSQEMVAEMNKRVITMDRGEVIRD
                     EGGTRR"
     gene            complement(22705..>22887)
                     /locus_tag="ACZPCL_02995"
     CDS             complement(22705..>22887)
                     /locus_tag="ACZPCL_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HIR1161486.1"
                     /translation="SLNVSETSRQLYIHRNTLVYRLDKLQKSTGLDLRVFEDAITFKI
                     ALMVAKYMKYMESLDY"
BASE COUNT         5959 a         6178 c         4130 g         6620 t
ORIGIN      
        1 aatcctttcc aaaccaattg gcaagctcac tttggactgt cagccgttcc ggaaggatca
       61 aaggcagatc tatcagatcc tcttttgtaa tatattcttt ctctgcaagg ggatcatccg
      121 gctttattcc tacgatccaa tgatcacttc cctggatccg gatgtaatcc agaccttctg
      181 tattcacagg ttctaaaaac aggccgatat ccaccagtcc acggttcatc atttcaaaaa
      241 cagtatctgc ggtacctgta tgcacggcaa aacggaccat aggatacttt tctttataga
      301 tcttgcatat ccgggcaaag atctccacag ccataaattc cccacagcct acggtgatcg
      361 tcccttctac gatcccgtca ctgcctttaa attcactggc gatcttatct tccagatcca
      421 ccatttccag cgcacggcgt ttaagaagga tcccctcatt ggtaagagtg atcccatgtc
      481 cgccccggtc aaaaagagtc actcccagtt ctgcctccaa agcagccagc tgcctggaaa
      541 gtgtaggctg ggtaatatgc agctgtttgg cagctttggt aaaactttgt tcctttgcca
      601 ctgtcaggaa atagcgcaat actcttaaat ccatataaac tcctcttttt atttcttttg
      661 ccttcgccca ttttttaata cctacaatta tatcattgaa tgttttcata aacaatgtta
      721 ctcttgatca ggcggatttt aatacaggag gtttttatgc aaaacaaaag acaagttata
      781 acaaaggcat tcaaatgtgc atttcctcat acaataccga tttttgccgg cttttgcttt
      841 cttgggatga catatggtat ttacatgaat gtatccggct tccgtttttg gtatcccatg
      901 ttaatgagcc tgactatttt cggcggcagt ctggaatttt tagcagtcag catgctgctt
      961 gcgccatttg ccccggtaca ggcatttatc atggcattga tgatccaggc acgccatctg
     1021 ttttacggaa ttgccatgct ggacaagttt aaaggaatgg gctggaaaaa accttacctg
     1081 atttttggaa tgtgtgatga aacattcagt atcaactata cagcggacat acctgctgat
     1141 gttgaccgca gctggtttta tttctttacc acacttctca accattttta ctggttcagc
     1201 ggagcgacca tcggcgggct tgtaggcagt ttgctaaaat ttaacaccga tgggctggat
     1261 tttgtcatga cagcaatgtt tacagttatc ttcctggaac aatggctgaa agaaaaacac
     1321 cacatcagcg agtggatcgg ccttggggca agtatcggct gcctgctgct cttcggcgca
     1381 gataattttc tgatccctac catgatctgt attttagttg ccttaacaat tcttagaaaa
     1441 accctggaac agagaaaaga ggtggatgca gcatgacttt atcacaacag atcattacta
     1501 ttgctatgtg tgttctcgga actatgctga cacgttttct gccatttgca gtcttcagtt
     1561 ccaaacgtcc aacgccgcca tacattcaat atttaggaaa agtactgcct ggtgcgatct
     1621 tttccatgct ggtggtctac tgcctgcgac atgtttcctt actgcagggc agtcatgggt
     1681 taccggagct gatcgcaatt cttctgacag ttggacttca cctttggaaa agacaaatgt
     1741 tgttgtctat tgcaggcggt acggtttgct atatgcttct tatccagttt atcttttaag
     1801 aaaaacgcgc atatgaaatg ccttaattat ttcagaaata accaaggccg gagatccaca
     1861 gcggatttcc ggccttactt tttgtttctt ttatcgttta tttcagttta ccatattctt
     1921 catcagaaac aggctcgcac cactcattag agccctcaac acctggtact tcaacagcca
     1981 gatggctgaa ccagctgtcc ggtgctgcgc catgccagtg ctttacttct gcaggaatat
     2041 tgatgcagtc gcctggtttc atttcaacgg cttcttttcc ctcttcctga taatatccac
     2101 ggcctgcgac acagactaag atctggccgc caccgctctt tgcgtgatgg atatgccagt
     2161 tgttgcggca gcctggctca aaagtcacat taaaaatacc aacctggctg gtagatacag
     2221 gtgccaggaa acttcttccg gagaaatact gggcaaaacc gtcatttggc gcaccgatgg
     2281 ggaataccat ttcttttgca tgggctctca ttgcctcatc ttcgtatgga aggtcgcctt
     2341 cattatctgc ccatacttcc tttgccagat taaatacagc ccagcccttt ggccagcctg
     2401 cgtaaaaagc aacatgggtg ataacggctg cgatctcttt ctgggttacg ccatgagcct
     2461 ttgcattctg taaatggaat tttaaagaag agtctgtgat accggatgcc attaaagcta
     2521 ctacggttat gatacttctt gttttcagat caatgtcctg attattccag ttttctccga
     2581 aaaggacatc atcattataa tgtgcaaaat caggagcaaa agttcctaat gcatttcttc
     2641 ctgctgtctg aactactttt ttcatctttc tgtctctcct tatctgtatg ataaactttt
     2701 aattataagg actttaccca cttttcaatt tcctgcttgt tggctctgtt taaaagcttt
     2761 ccttctacga tctcagctcc cggtgcagat ggctttaatt cctttactgt attgccaaaa
     2821 ccgcttcctc cggatgttgc aaagagcacg atcttcttac cggaaaaatc atatttctcc
     2881 aggaatgtat ttacaatggt aggtgccaca taccaccaga tcggaaatcc caggataatg
     2941 gtatcatatt cttcaatatg agcatctgta tctgtgatct gtggacggat cttcttatcg
     3001 ctcatctcca tgctgcttct ggatttctta ttcatccagt ttaaatcgtc atttgtatat
     3061 aattcctttg gtgtgatctc atacagagca caatctcctg cttctgcgat catctttgct
     3121 acttttctgg ttgttccgct tgcactgaaa tatgctgcta atgttttttt catggctaaa
     3181 acctcctact gttgcttaac aatgaaaaat aataaaatga aaaatgtgca ggacctttta
     3241 ctttcatcct gcacatttat tatataactc ttttttctat tatgtctaat acttatatta
     3301 catggttttt tatgcttaaa aagcattatc ctgaaacaaa ctcatctatc cctcaaacac
     3361 aggatatttt ttcttatcag catctgttat tttgcggatc accttacagg ggttgcctgc
     3421 tgcgatcaca ccatccggaa tgtccttatt cacaacactt cctgcgccaa tgatcgtatt
     3481 acttccaatg gttactccca gcagcaccga tacattggtt ccgatccaga cattatctcc
     3541 tactgtaatt ggcagggcaa tttccagtcc cctacttctc tgttcactgt caatagcatg
     3601 tccggctgta gaaaatacgc agttgggcgc aataaataca ttatccccaa aagtaacttt
     3661 tgcacagtca agtatggtaa cattatggtt cgtataaaaa ttttttccta tagaaacatt
     3721 aaaaccgtaa tcacagtaaa agggagctgt gatcaccgga tctgctccca ttcttccaag
     3781 gatctgtccc agtaatttct gctgtttttc agtatctgat ggcctgcagt tattaaactc
     3841 atagcacaga tccgcacagc gtgttcttgc ttctatgatc tccttatcgt aatttgcatc
     3901 atacagatag ccattttgcg ccttttccca ctcagtcatt gctctgtctc ctataccctt
     3961 tcatcatttc tcttcatata caaaagggga tagggatttc cttcctcatc catatctgtc
     4021 cttttgtatg tttcaaagcc catatgctca taaaagccaa ctgcctgagg attttgctca
     4081 tttacagtca gttcctctat cccgtaattc tggatcccgt ataaaagcaa ttgttttcca
     4141 aggccttttc ctctttcttt ttgggaaagg aacaacattt ccaggcggtg gtcttctatc
     4201 cccataaatg cagctggctc acctgaacca ttgaccgcaa taaccaggct ctgtacaccg
     4261 gctaatgcct gtgggacata ctcttttatc ttctcaattt ctttttcaga aagaaattga
     4321 tgagttgccc tcaccgactc ttcccacacc tttaaaagct gttccatcaa caattctgtg
     4381 cgctctttta cttcatatat tttcatattt accatacatt tcaaaatgta ccttatgttc
     4441 tggaacttca gataacttac ggccttccgg atgcactgcc ggtacaccga tcgataagac
     4501 cgcctccaga caatattcct ttggataacc taaaatatct cttagatatt cttccgttgt
     4561 tcttccatca ggtgcctgtc ttaaacgtcc ctggatccag cagcttccaa gccccaggtc
     4621 gtgggccatg ttgtgcatgt tggacataac aatggaacaa tcctctgtcc atacgtctgt
     4681 cttttccgga tctgcgatca caacgatcgc aacagacgcg cctgccagca tttttgcaga
     4741 acccacgcgg cactcagaca ttttttcaag tgtttctttc ttctgtacta cgatcagttc
     4801 ccatggacgg atatttcgtc cggatggaga aagcagacct gcctcaacga tggcctccag
     4861 tgcctcctta gaaactgctt cctctttata ctgtctgata ctccgtctat gctttaaaag
     4921 atccaaaaaa ctcattaatg caacctcctg taaacaattt atctgttatt cttcctcagg
     4981 atacgtaaaa tgccaggact ttctaaactc cgtcatcaga tccattacat ccttcgtata
     5041 gtccggatac atacaggaaa catccccggc aatcgccttt tccatatcag aaagctcata
     5101 agccagtgca tcctcatgaa ctccagcctt tatcacctgg gtttctccgc tttctgcgtc
     5161 cacgatcttc gcctcccagg cccttggata ctccatgatc tcaatatagc ccttttcgca
     5221 ggataccatg cctctctttg gctgcttgga gtgcagggac agcatcacgg tagccatctg
     5281 tccttcctta ttggtaagaa gcagccccgc ctgctcatct acccctgtag gcgccgcctt
     5341 catctgggac aataactgat ccggcttgga gtccataaac caccggataa aagaaagagc
     5401 atacacgccg atatccagca tggcaccccc agccaggttc cggttaaaga aacggttttc
     5461 catgttatat tctttaaaac ttccaaaatt catggtgatc agatttactt ttcccagctt
     5521 tccggactcc aaaatcttct tcaattcttt atagaccggc atatggaaaa tggtcatggc
     5581 ttcggcaaca ataaccccct tctcctttgc cagctccaaa gcttcatcca gttcctcact
     5641 atttaaagta atagatttct ccaccaggat atgtttccca ttttcaatgg cctttttcat
     5701 aaactggata tgagtattat gaggcgttgt aatatagacc acatccactt ccggatccgt
     5761 gaacatttca ttaaaatccg tatacacctt tttgatcccg tatttttctc caaactttac
     5821 agccttttcg taggtcctgt ttcctactgc gtaaagcttt ttgccatttt tttccagtgc
     5881 cgttgccatt tcattggcga tcacacctgt tcccagtaca gcccagtgaa tttctctcat
     5941 cctgcgctct cctttcctgc gaaaatattt tgccagtctt ccttatcgga tctctccttt
     6001 tctggctctc ctgttcagta taaccataaa tccaaggcaa agcaatagct gcacaacaga
     6061 tgacataggc gttgccaatc cgattttaaa taaagaagta tccggtcgga tgctcatgag
     6121 ataagataca ggcacacgaa ctccaaaagc cccggcaatt ccctgtaaca taacaaattt
     6181 ggtctgtccg atcccattat aaagggcctt ctttgcccgg tccatccttc ctgcgccata
     6241 attctgagcc acaaaagcag acatagactg cataaaagca gatgaaataa gcatgataaa
     6301 agcacaaact ttctcagcca ctcccacacc tgcagaagcg gtcactccca tggaatttac
     6361 gatcgccagg ataataagaa acgaaatact taccagaaga tcctgtaatg cgatcggcgc
     6421 tccaaggccc gccattttct taagtaacgt cttgtggatc cgcacattct ccttcttaaa
     6481 cacaaagggc agctgtttct ttctgataaa tccaaaagac acgaccacgc tgactacctg
     6541 tgcaaaaaca gtagcacagg ccgctcctgc agttcccata tgaaaaccgg cacacagcac
     6601 cagatctcct gcaatattac aaacacaggc gatcgcaact gtaaaaagcg gcgttttgga
     6661 gtccccgatc cccctgaaaa tacaaccgat caggttataa gcaacaataa cgatcatacc
     6721 gcagccgcat atccttacat aagcagccgt cttgtcaaaa gcctcctttg gagcatgcat
     6781 aatactgctg atctgaggtg caaaacacac cagaaggaca gtcatgacca atgcaatacc
     6841 agcaaacatc accatggccg ttcccactgc ctttccccct tcttccttct ttccgcagcc
     6901 gatcagctgt cctaaaagca ccgtggttcc catagcaaaa ctgctgatca gattggtcaa
     6961 agtcatcatg atctgagaac ctgtagacac cgccgataca tccgcagaag acgcaaactt
     7021 acccacaacc aggagatcca cagcgccgta catagcctgc aaaaacagcg caaacaacac
     7081 tggcatggca aacaaaataa gaggctgtaa gatcttccct tctgtaaatg tcgtatttct
     7141 ctccattgta cattttctcc tttttcacat taaaaaagcc ggataagaag caggcatctc
     7201 agcccgccat cttatccagc tattgtttct aaagaacaac ttgccctccg atgaacgttc
     7261 cacatatact ataaaaattt tcaaacatca actgccagat ccacatcttt ggctcaaatc
     7321 atttctatta atctctgtca ttcagataac ctggttcatt ataaataaga gccataaact
     7381 ctacatcaga gtcagaatta ttctcaatgc tgtgatactg tcccacttca ataatacagc
     7441 agtcaccagg gccaacctta gtcacagact tgtcattatc aataaaatct gcttcaccct
     7501 ttaaaatata atatggctca gtgtcatgta catgctgatg ccagcctaca cttgcacctg
     7561 gaggaagaac taccttagca tacagtctgc cgtgtcctaa cagctcctcc ttagatacaa
     7621 tatgatgaac atacgctgtt cccttacctc cgcctaaatg ttccacttcc agaacgttgt
     7681 ctttacgaat catggtcatg ccctcctgtt gaatataatc ctgaagcaat ttgaaacact
     7741 ttactgctta tattttctca ttatactcca tttctctcat ttttccaaga acaggacgcg
     7801 atttttcttt cgcgaatgtt tcattttgca gcaaccatct gctcttattc cctttcctgc
     7861 gcaaacttct gaaatgcctt ggctgccaca cttaaaggat actcgctgtc ccgtatcata
     7921 agaacattcc tttccggaaa tgcctcttcc agatcaatgg caaatacaac acctgctttt
     7981 aagtcttccg ctgcaatctc ctccggtata aaaccaattc ccagattatg acgcactgcc
     8041 ggaaggatca gatccgtagt ggccagttcc agatccggtg aaaatgtcag gccatactgc
     8101 aaaaaatagg aattcaggaa attacggctg atcgcctcgg aagtaaggct gatccacggc
     8161 agatctgaaa gctcccgcaa agaacatttt cgtcccttta actgctcaaa tctggggcct
     8221 ccgatcaaaa tctcccgata gctgcgaagc actttcattt tcaatggatc ccccatatgc
     8281 aaaggaagag acgaaaccac tgccatatcc accttaccat cacgaactgc ctggatcgag
     8341 tccctggtac tgttatttaa gatctgaaaa tggaccttcg ggtacttccg gctaaactcc
     8401 tccattgcct gaaacagata acaatgaagg gctgtctctg tggcactgat cgagatcgta
     8461 cccttttcca ggctcagcct gtccttcaga tcactttccc ctttaaaaaa ctgggcgcag
     8521 cctgctgaaa catactcaaa aaactgctgt ccctctgccg tcagctccat ccccatcttg
     8581 ctgcggataa ataaccggca ccccagatca tcctccagct tatgtatggt ccttgttacc
     8641 gccggctgac tggtattaag ggcagatgca gccctggtca gatttccata ctttgcaaca
     8701 taataaaaaa tcctgtagta ttcaaaatta gatgtcataa ctaatgtccc ttctatctag
     8761 ctagcttcga ctcacggaca cgcgctaacg cgctcatctg tctgctgacg cagacggtcc
     8821 gttcgtgaat gacgcaagaa aaacaaatgt cgccttgcag gcgccgcttg tttttctttt
     8881 gcgcctttcc ataccattct gatatgaata taatacattt ttgctatttt acaacccttt
     8941 ttattcgtgg catattatat gtgaaaaaaa taacgtgcat ttgaaagggg caacatcatg
     9001 gaaaacaaca ccggcacatg tcttgaaaag cagcctttat gtgaggaaat gcttacaaaa
     9061 atgaacgcat actggcgggc agccaactac ctgtctgcag gacagcttta cctgttagac
     9121 aatccgcttt taaaaaagcc attatccctt gaccagatca agaaaaagat cgtaggtcac
     9181 tggggaactg tacctggaca gaactttgtc tatgtacatt gtaaccgtgt gatcaaacgt
     9241 tatgacctgg acatgatcct tttatccgga cctggacacg gcggcaactt cctgatcgcc
     9301 aatacctatc tggatggaag ctacagcgag atctatccaa acatcagcca ggacgaagaa
     9361 ggaatgcaga aaatgttcaa gcagttctcc ttcccatgcg gcgtgccaag ccactgtgct
     9421 cctgaaactc caggttctat caacgaaggc ggcgaattag gttactccat tgcccacgct
     9481 ttcggtgctg tattcgataa cccggatctg atcgctaccg ttgtagtagg tgacggcgaa
     9541 gcagaaaccg gcccactggc tacttcctgg cagtccaata aatttttaaa ccctgtaaca
     9601 gacggcgctg ttcttcctat ccttcactta aacggataca agatcagcaa cccgaccatc
     9661 ttctcccgta tcccacatga tgagattgaa gatttcttca aaggctgtgg ctggaaacca
     9721 tacttcgtag aaggcgacga tccaatgacc atgcaccgca agatggccga aaccatggat
     9781 accgttatcg aagagatcaa agccatccag aaacatgccc gtgaaacagg agatgcagag
     9841 cgtccaaagt ggcctatgat cgttcttcgt acaccaaagg gctggaccgg accaaaggta
     9901 gtagacggcc agaagatcga aggttccttc cgtgctcatc aggtgccgat ctccatggaa
     9961 aatccggaag aacacttaaa actgttaaac gactggttaa gaagctacca tccagaagaa
    10021 ctgtttgacg aaaaaggccg tctgatcccg gaattagccg aactggctcc aaagggaaat
    10081 gcaagactgg gcgcaaaccc acatgcaaac ggcggacttc tcttaaaaga cctgcgtctg
    10141 ccagatttca gaacctacgg aattcctgta gaacctggaa agaccaaagc ccaggatatg
    10201 atcgaactgg gcggatacat ccgtgacatc ttccgcttaa atgaggacaa caaaaacttc
    10261 cgtatcttcg gacccgacga aagcatgtcc aaccgtcttt accgcgtatt tgaggcagaa
    10321 aaccgtgact ggaacggtga attaaaagat gacgatgaca aactggcaag aggcggacgt
    10381 atcatggaca gtatgctcag tgagcatatg tgtgagggat ggctggaagg ctacctgctt
    10441 acaggacgtc acggtttctt tgcaagctac gaagcattta tccgtattgt tgactccatg
    10501 gctgcacagc atgcaaaatg gttaaaagta tgcaaccagc tttcctggag acagccgatt
    10561 gcttccttaa acttcatcct gtcctccaat gtatggcagc aggaccacaa tggttacacc
    10621 catcaggatc caggcttctt agatcacatt gcaaacaaaa aagcagacgt agtccgcatg
    10681 tatctgccac cagataccaa ctgcctgctc tcctgctttg accactgcat ccgcagcaaa
    10741 aactatgtaa acgccattgt tgcatccaaa catccaagct gccagtggct gtccatggaa
    10801 caggccgtaa agcactgcac ccagggtatc ggcatctggg aatgggcaag caacgactgc
    10861 ggcgaagaac cagatctggt catggcatgc tgcggtgata caccaaccct ggaaacaatg
    10921 gctgctgtca ccattctccg tgacgaactg ccggaattaa agatccgtgt tgtcaatgta
    10981 gtagacctgt tcaagctgga gtccaacacc aagcatccac acggcttaag cgatgccgaa
    11041 tatgacgcga tcttcaccaa agatactcca gtcattttcg cattccacgg ctatccaacc
    11101 ctgatccacg aactcaccta cggacgcaac aaccacaacg tatccgttca cggctatcag
    11161 gaagaaggta ccatcaccac tccattcgat atgcgtgtcc agaaccagtt agaccgtttc
    11221 gaccttgtaa aagacgccat catgcatctt ccacagttag gaaacagagg ttccttcctg
    11281 atccagaaga tgaatgacaa actggtagaa cacaaacagt acattgccga atatggccag
    11341 gatctggaag agatccgcaa ctggcagtgg cacactccag aagacaagta aaacaagtat
    11401 ataaaaatac cgcctgggca gcaatccaat ccttcgctgt cccagacggt attttaataa
    11461 tcacacttat ttttcagtta ttccttgtat acacaggccg tcatcttact tgtcaattgt
    11521 ccacatcttg agcgcagctg tccgaatgta gcggacgtag tccgatacag gcgaacaggt
    11581 gcatgtcgaa agccagtgga caatgacaag taatatgtaa ggccgtttct tacggaaaag
    11641 cgttgctccc ggaacccttc ctcacggctg ccacaaactt cgccattttc aaagcatctt
    11701 tcaccccaaa gctttcaatc ccagaactca catccactcc aaatggtcgc agcctttcca
    11761 ccgcatccct cacattctcc ggatccagcc caccagccag aaaataaggc cgtttcacat
    11821 tttccagtaa tttccagtta aacaccttcc catcaccggc gccggcatca aacaacacaa
    11881 gatccgcccc ggactgctct cccgctttca catccgcttc cgacttcacc tgaaccgcct
    11941 ttataataaa cgtatcttca cctctgccag taagttccct cagccgacaa atatacgcct
    12001 catcttccac tccatgaagc tgcgccatat caacgatccc ttcattcaaa agatcagcca
    12061 cattttccac cggctcattc acaaacacac ctacagcctt caccgccgga tccagcattt
    12121 ccttaagcat tttcgcctgc tccttagaaa catatctgcg actcttcttt gcaaacacga
    12181 acccaatata ctccggcttg atctcattca ccgcctggat atcctccggc cgcatcattc
    12241 cgcacaactt cactctggtc atctgtttat actcccgctt tgtcaacaca catacttatt
    12301 ccgttttcta acctctcagc gccgccaatt tcgccttctt atcctcagcc cgcatcagtg
    12361 cttctccaat aagaactgca tctgccccaa tctcccgaag ctttctcaca tcattctcat
    12421 cccttacgcc actttctgat acaaaaagca cctctgccgg tatcatgctg cgaagccgtc
    12481 tactgttttc cgtatccaca gaaaaatcct tcaaattccg gttgttcact ccgatcattc
    12541 tggcgcctgc ttcaacggcc atttccacct ctgcctcatc atgagcttcc accagagcag
    12601 aaagtcccaa ttcatcacag atacacctgt attcctttaa ctgctccttt gacaaaatag
    12661 aacagatcaa aagcacagcc gaagctccca gaaccttcgc ctcataaatc atatattcat
    12721 caactgtaaa atccttcctc aagcagggaa ttcccacact ctcagcaatc tctctcaaat
    12781 aagcatcact tcccaaaaac cactttggct ccgtcagcac cgaaatgcag tccgctcccg
    12841 ctgcttcata ctcctttgcg atctttaaat aaggaaaatc cggtgcgatc agtcccttag
    12901 aaggagaagc cttcttacac tcacaaataa acgagatccc aagcttctta agagccttct
    12961 caaatgcaaa actgcccagc ctgccctctc ttccaagatc aaaggcccgc cttctcatct
    13021 catccgcagc cactttctct ttcccatatg ccacccgctc cctggcatga tccgccagct
    13081 gatccaaaat cgtcatatta ttccgcctct ccgttactca cttcaatcag tttctccagt
    13141 gtatccagtg cctttccaga atcgatcagt tctgctgcca gggcaatacc ggacttcata
    13201 tcctctgctt cccctgcaat atacaaagca gcacctgcat tcatcagaac agcatttctc
    13261 ttatgaccct tttcacctct taaaatagac cgtgtgatct ctgcattttc tgccggtgtg
    13321 ccacccttaa ggtcatcctt tgtacaccgt tcaaagccaa actcctcagg agtaataaca
    13381 gacgttctgt accatccatc tctgatctcg caaatggtag tcggcgcact taaagagatc
    13441 tcatccaact tatcctgacc ataaacaacc ataccacgct taattcccag acttaccaga
    13501 actcttgcca gaggctcaac cagatattca tcatatacac ctaacagctg catggtcggt
    13561 gttcccggat tggtcagcgg tcctaaaata ttaaatacgg ttctaaatcc cagctctttt
    13621 cggatcgcgc caacatattt catggaagta tgatatttct gtgcaaagaa gaagcacatt
    13681 cccacttctt ttaataattt tctgcaaaga tccggactct gctcaatatt aactcccagc
    13741 gcctctaaac agtccgctgt tccgcacttg gaagaagccg ctctgtttcc gtgttttgcc
    13801 acttttacac cgcctgctgc tgcaaccagg gccgctgtag tagaaatatt aaagcttcct
    13861 gcattatcac cgccagtacc aacgatctca aacagatcca tgtctgtttc cactttggta
    13921 gcatgatctc tcatagctgc tgcgcagcct gcgatctcat ctgtcgtctc tgctctggca
    13981 ctttttgtag aaagagctgc cagaaatgcc gcattctggg taggtgtagt ctctccgctc
    14041 atgatctcat tcatcacggt atatgcttct tcataactta aatcctcttt atttacgatc
    14101 tttacaatag cctctttgat catcgttctt acctcctgat attctgctca ttttaaaaat
    14161 ttcaaaccgt tcaaaacatt ctgtactctc ttcccgctct cgtcatcccc ctgctgacgc
    14221 aggaggaaag ctgaacgatc aatgttccag gaaattctgt atcatccctt tcccatccgg
    14281 tgtcataatg gactccggat gaaactgtac tccatagatc ggatattcca catgctgtac
    14341 tgccatgatc tccccgtcat ctgtcactgc tgttaccttc agcacatccg gcattgtctc
    14401 cggatccgct gccagtgaat gataccttgc caccggcgcc ttctttgggc atcctttaaa
    14461 cagcaacgac tctgtatcaa acgtcacctc cgactgtttt ccatgcatca gctccttcgc
    14521 ataagtaact gtagctccaa aagcagcgca gatggcctga tggcccaggc agacaccaag
    14581 aaccggtatc tcttttccca acgtttttgc cacatccacg atcacccctg cgtcctccgg
    14641 tcttccaggt cccggtgaaa ggatgatccg ttccggtttc aactgccgta tctcttccac
    14701 tgtcatttca tcattgcgaa tcaccttaat atccgggttg atctcaccga tcagctgata
    14761 aagattatag gaaaaactgt cataattatc gattaataag atcataattc ttcctcctgt
    14821 gccatttcca gtgcctttaa tgcggctctt gccttattta aacactcttc aaactccttc
    14881 tttggatctg agtctgctac gataccggcg ccgcttctta caaatacctt gccatttttc
    14941 ctatatgcaa tacggatggc gatacaggta tccatatttc ctgtaaaatc aatatatcca
    15001 atggctcctc cgtaaatgcc acgtttatta ttttccagtt ccccgatcag ctggcaggca
    15061 cggatctttg gcgcaccgga taaggttcct gccggaagca cagcctcaat ggcatccagt
    15121 gcgtcatgct tctcatcaat ctctccccgc actgtggaac caatatgcat tacatgggaa
    15181 taccgctcaa tggaatgaag tttctctact tttacagaac caaacttact gatctttccc
    15241 aggtcatttc ttcccagatc cactaacatg ttgtgttctg ccagttcctt cttatctgct
    15301 aaaagctcct cttccaatgc cttatcttcc tcatccgtct ttcctctcgg tcttgttcct
    15361 gccagtggga atgtatggag cacgccatct tccagtttta ccagtgtctc cggtgaagca
    15421 cctgccactt ctacatctgt tcctgaaaaa taaaacatat aaggagacgg gttcaatgtt
    15481 ctcagtaccc ggtaagtatt taataaactt ccctcaaaag gagcacttaa gcggttggaa
    15541 agaacgatct gaaaaatgtc tccttcccgg atataatgct ttgccttttc cgccatggca
    15601 caatactgtt ccctgttaaa taaaggtgta acttctcctg tcagcttacc ccccggttcc
    15661 tgctttttct ctcctttttt cagaagttct gccagctgct ttaactccat tacagccttg
    15721 ttatatccaa cttccacatc atccaaagac atgttcacca ttaaaatgat cttctgtctt
    15781 acgtgattaa aagcgatcac cttgtcaaaa agcatcaggt ccacatcttt gaaattttcc
    15841 gtatcttcca ccttgcaccg cactgccggt tcgctgtatc ccagatagtc ataggaaaaa
    15901 tatcccacca gaccaccggt aaatggcgga aggtagtcaa atcttggact tttatagtcc
    15961 gctaatatct gtcttaaaaa tttagaagga tcctctatct ttacggaaaa actgcctgct
    16021 ttcatttccc cgtccctgca ggtgatctcc attttcggct catatcccat aaatgtgtac
    16081 cggccccatt tatcatttgc catggctgac tccagcatgt agcaatgggt agaaacattt
    16141 ttaaggatct tcatggtttc aataggtgtg gtaaaatcag aaagcatttc acagcttaca
    16201 ggcagtacac ggtattttcc atctgctgcg atctttttaa cttcctcaag ttccggaaaa
    16261 aacttcatag aatgacctcc ttatgcacaa ttgggataaa caaaaaacgc ccctgacaga
    16321 atactactac tctgtcaagg acgaatacaa tttctctaaa atatgatccg cggtgccacc
    16381 ttgattcacg gtataagccg agctcttagc aggatacaac tatatccccg gcaaatgacg
    16441 tctgcctgca acgtcgcaga atactcagca tatgcaccag acaatatccg gaacttcgct
    16501 tttgactgcg ccctcagcgg tccatttgac aatgtgtttc ttacctgaat tccaccacct
    16561 caggctctct gtgaaggcat catagccgtt atctccgctt catcggtttg aagtattaaa
    16621 ttggttggat tatagcatag ctttttttgt aagtcaatat gtctatttaa aaaattttgt
    16681 caacttttaa tttgcctttt catacacact gaacccgttt ttgccgccct gcttcacctg
    16741 atacaatgca gtatctgcat gacggtacag ttccataaac gttgttccgt gttccggtga
    16801 aaatgccaga ccaacgctga tggtaatgcc atttccatcc atctccggta tctcaaggct
    16861 tctcacctcg tcgatcagct ttttcaccct gttttccgca atacttccat tatttatatt
    16921 ccggatcagg accataaatt catcaccgcc aatacgccct gcaatatcgt gctccctgaa
    16981 ctgtcttgca agaagatgtc cgaatgtctt taatacctta tcacctacaa tatggccata
    17041 ggtgtcattg acctgtttaa aatagtccac atccataatg agaaggccgt tacagctttc
    17101 tgcctcccct tcttctaatg acctttcgat cagctcctgc gttgtttttt tattgtaaag
    17161 ccctgtcagt gcatctctct gtgcccgttt taacagtctc gccttttcct gccggttttt
    17221 ataaacaata aaaaggacca tggctgctat caacattcca aagacaatga taaaaatgat
    17281 ctcataatcc ctgataaagt cataggaagc ttccgctgcc ctcacaggaa caatataact
    17341 gatcatccag tcattcatcc caagaggggt ataggcaata taataaaagg gctttctccc
    17401 ggcagaaaag cggaattcac gaattccctc ttctccagcc gcaaaatctg tcttaatgtc
    17461 ctggattgca gctgtcctga tctgacaatt atcaaaataa tcaaatatat tgccggaaaa
    17521 tacctcctgt tcctgctctg aatacactct gtcataagtg atcacgtttc catctttatc
    17581 tgtgatcagg ctgtagccat ttcccccaaa caggtcatca aacatcatgt gacttaaagc
    17641 cgtaacatta taagaaccgc ctaaaatacc ggcaatctcc ccattcttta ataccgggac
    17701 tcccaggacc actcttactt ctttatcaac actgctctct attgggtcac ttaaggtctg
    17761 ctgcccgttt acagcctcat taaaatatct gcggtgggat acatttttca catccccggt
    17821 atcataatga gccgtaccgt ctgtttcgat cagtgccacc cggtccagat ctgttttatt
    17881 gtggatcgta accagagtat ccagattgat ctgagaaaac agttcttctt cctttgccat
    17941 ctcttccgcg atctcattca gatactggta atgaatgctt aagattgtcc gtaaatgctg
    18001 actctggcgt gaaacattgt tcatcatggt ctgcctggaa tttttcccta cagaagactg
    18061 tacgtagcca aaaaatatca tgatccccag gatcattgac aaaataaata atcccgtaac
    18121 catcgtgtaa tgcttataat aagttgtttt ctttttcatg cctttatcca cccccccccc
    18181 aacccctcaa aaaaaaaacg tcaatacttg tgcatatcgt acactatcta cacacctgat
    18241 tatagcaaat acacgacaga aaagctacct gatttatgcc tgtttactta ctatttatat
    18301 acgatttcca tacgttttat ctacaaaaaa gccaaggtct gcataacatc tgactgctat
    18361 gctgcctcag ctttttcaca tactctttaa ttttctttca gtgcttccat tgccgcctgc
    18421 acctggttat cttcctcagg tgtaactacg gactgactct ttaatttttc atctaactct
    18481 acttccacat ccggggtaat tccctttcca tgaaggtcaa aaccgcttgg tgtatagtaa
    18541 tgagccgtgg tgatcttaat ggcggatccg tcacctaagg ggatcaggtt ctgcacaatg
    18601 cccttgccat agctggtggt tcccacaact acagctttct tgtaatcctt taaagcccct
    18661 gtaaatacct cagaagcgct ggcagagttc tcattgacca ggatcgcaat aggaacatct
    18721 acttcgtgtc cgtcagcagc tgtgtaggtg tctccctttc catttttatc tgctgtatac
    18781 acgatcaggg tctttccttc gcccttttca aagtctttcc tgtcatctgg taaaatgtaa
    18841 tctaccatgg caacagcgct gtctaacata ccacctggct tgctgcgaag atcaatgatc
    18901 atttgcttca ttccctggtt ttccagttca tcaatggctt tttcaaactg ctccactgta
    18961 acagcgtcaa attcacttac tgccacataa cctgtattgt ccgcaagcat ctttgactct
    19021 acagtaggaa cttcgatctt acgtctggta atggtcatat cctcataggt atcagactct
    19081 gccctgtata cggtgatctg tacatctgta ccttccttac ccttgatata attacttacc
    19141 agtacatcca ggctttcccc tgctaccaga gtatccccta ctttgtatat gatatccccg
    19201 ggtttcatac cagcctccag agccggtgaa ccttcaaata cctttacaac cgtacacagc
    19261 cccgtcgttt tattctggct gatcatagct ccgatcccgg aatagcttcc gtctgtgctt
    19321 tcctggatcg ctttatagtc ctctgctgta taatacacgg aatatgggtc atttaaacct
    19381 gccatcattc ctctgtaaat atactcttcc acctctgtgg catcctcatc gtataaaaat
    19441 acctggtcga taatgctctg tatcatctgc atttttgagg ataccttttt cagatcaggt
    19501 ttgcttgcct tttcgccgtc cgtgataccc ggagcaccat ttcctgtacc ggaagactgc
    19561 tctgtacgga tcacttttct gcctgtaata taaataccgg cagatacacc tactaccaaa
    19621 agaacggcca atgccatgat cagggcacct acaaggaccc ccgcccagaa gcggccgcct
    19681 tttcgccctt tctttttatc tatgctgtct gagatctctt ctctgtcctg ttcttccact
    19741 gctccgctct cctatctgcc tgtgcctttt tcttattcac agcctatgct gttttttcct
    19801 gcaaaatgcc cttcaaagga ctgtcccttt cctatgggct tacatattta cttggattta
    19861 cataacttcc accggaacgg actccaaaat gtaaatgaga accggtagaa tatcctgtag
    19921 agcccactct tgcaatggtc tgtccctttt tcaccttctg tccctggctt gcaagaagct
    19981 gggagcagtg catgtaaacg gtgctgacgc cgccgccatg gtcgatcata atataatttc
    20041 ccgctgaata actgtatgta gcgatcacta ccgtaccatc ggcagctgcc acaatggaag
    20101 ctcctgtact ggccgaaata tcaataccct ggtgattgct ggacgcgccc ttggtaggcg
    20161 actttcttcc gccaaaaccg gaggtgatac gtccgctgga aggacatggc catttaaagc
    20221 taatattccc aagattagtt acgttatacg tctttttctg ggcctccgct ttctttctgg
    20281 cctcttcttc ctttcgcttc atctctgcct caatggcctt catctggttt tcctgggctt
    20341 tgatatccgc attatactgg tcaatactac tctgagccgc accgatctgg ctgttgtaag
    20401 aggtcagttc cttctgcttg gaactcatca gctcctttac agaagcctgt tttgcttccg
    20461 tagactcctg aagtgctaac agattggaat gttctgtttc cagatcctgc tctttctgat
    20521 ttatcttttc cctggtagcc tgatattcct ttaacatttt cctgtcataa gtagcgatct
    20581 ggctggcata ctctgcacgg tttaacagct gggaagcgtt tttagaggtc ataatgctgt
    20641 ccgcaaagct gacatttcca ttttcataca tatactggat acgcagtttc atagaagcat
    20701 actgctcctt ttccactgct tttgcctctt ctaactccgc ttttgtattc tcgatctgat
    20761 cttcctttgc ggaaatatct ttctctaact ggctcagttc ggagtctaag gaagtcaact
    20821 tcttatccag ttcctttaca taagcagctg catctgattt cagtccttct aactgtttta
    20881 aggtatcctg gaccttcttt ttttcctcct gaagagatga aactttcttc tttgcatcct
    20941 tcagatccgc actggtagca taagaagaaa atacacagct aaggccaaga acgaccccca
    21001 gtccaagagc cagaaatttg ttttttcggt tcttcatctg ttccttcctc tctacacacg
    21061 cagatgtctc tgtatggtca gaaagcttac aatagctccg atcccaacgc ccaaagccat
    21121 agctgcacct gccattacag gaaagatctg ttttaaaggc agcagttcaa aaagtcctgt
    21181 gatcatctgg taattggtat tcagatacac cagtcctctt ttataaagaa tggtcattcc
    21241 agccagaggg atcactgctc caaagagccc taaaagcagg ccttctacaa taaaaggtgc
    21301 ccggatcata aaattcgtag cgcctatata gcgcatgatc tcattttccc tccgtctgaa
    21361 ggcagctgcc acagatatgg tattgctgat caggaaaata gcaactgcta acagcacacc
    21421 aatgatcaca gcagataaag cgccgatcat ctttcctgcg cttgtaaagc ctgccaccaa
    21481 agcgctggaa tagcggactt ttcgcacgcc ttccatacct tctaaccgct ttacgatcat
    21541 atcctgatta gaaatatctt ttaaatagat ctcataagag gaggaatttg ccagaggatt
    21601 atctgcctca aaaccttctg ccagctcttc cattccctca aaatattcac ttttaaagga
    21661 ctcccatgct tcctctgcgg atatatacct tgcttcccgc acttcgctcc agctttgtat
    21721 ctgtgatcca atggacctga tctgttcttc atccattcct tcatcaaaaa acacagagat
    21781 ccctactgtc tcttccgcag ccctggctcc gtgctgtaca ttggccacca tggcaaaaaa
    21841 cagacaaaat aaaaaaatac atgcagaaat ggttgccaca gacgccaggg aaaaccatat
    21901 attcctgcat atattaataa cgccctgttt caggcaatat aaaaatgtac tcatttactt
    21961 cttatgcctt tccggcggtt ccaggccgtt tctatctcct ggttccgccc tcatcccgga
    22021 tgacttcccc gcggtccatg gtgatgaccc gtttgttcat ctccgcaacc atctcctgac
    22081 tgtgggttac tacgatcacc gttgttcccc tctggttgat ctcatccaaa aggttcatga
    22141 tctcatgggt attgaagctg tccaggtttc ctgttggctc atccgccagt aaaacttccg
    22201 gcttgttgat caatgccctt gcaatagcca ctctctgctg ttcgccgcca gacaactgac
    22261 ccgggtatgc cttatattta gaagaaagac ctaccaggcg cagcatctgg ggaaccgtct
    22321 cgcggatggt cctggatgga acaccgatca ctctctgggc aaaagccaca ttttcaaata
    22381 ccgtccggtc ctttaaaagg cgaaaatcct gaaaaactac ccctaacttt cttctgtatt
    22441 tgggaacata tctgcggggc atcttcccaa gatccatatc attgactaca atacgcccct
    22501 tagtaggatc aagctccttc atcaggagct tgatcaatgt actctttccg gaaccgctgc
    22561 ggcctgtgat aaacacaaat tccccgtcat caatggttaa agacaggtca cggatggcct
    22621 tgctgccgct tccgtaagat ttacttacat gacttatttc tatcattttc taacttacct
    22681 cttacgtgcc agaaccaccg gaaattagta gtccaggctt tccatgtact tcatgtactt
    22741 ggcaaccata agagcgatct tgaaggtgat agcatcctca aagacacgaa ggtcaagacc
    22801 ggtactcttc tgcagcttgt ccagacggta aaccagggta tttctgtgga tatacagctg
    22861 tctggaagtt tcggatacat ttaagct
//
LOCUS       DBMDUA010000020        22818 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1470_length_22818_cov_2.911608, whole genome shotgun sequence.
ACCESSION   DBMDUA010000020 DBMDUA010000000
VERSION     DBMDUA010000020.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 22818)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22818)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22818
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1470_length_22818_cov_2.911608"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            173..1561
                     /locus_tag="ACZPCL_03000"
     CDS             173..1561
                     /locus_tag="ACZPCL_03000"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR1161487.1"
                     /translation="MEKTMEKIVALAKNRGFVYPGSEIYGGLANTWDYGNLGVELKNN
                     VKKAWWQKFVQESPYNVGVDCAILMNSQTWVASGHLGGFSDPLMDCKQCKERFRADKL
                     IEDYNDEHGIEIEGSVDGWSQEQMKQYIEDKHICCPSCGAHDFTDIRQFNLMFKTFQG
                     VTEDAKNTVYLRPETAQGIFVNFKNVQRTSRKKVPFGIGQIGKSFRNEITPGNFTFRT
                     REFEQMELEFFCKPGTDLEWFAYWKQYCIDWLQKLGIKPDEMRARDHSPEELCFYSKA
                     TTDLEFQFPFGWGELWGIADRTDYDLTQHQTVSGQDMTYFDDESKEKYIPYVIEPSLG
                     ADRVTLAFLCAAYDEEEIGEGDVRTVLHFHPALAPVKVGVLPLSKKLNEGAEKVYAEL
                     SKYFNCEFDDRGNIGKRYRRQDEIGTPFCVTYDFDSEEDHAVTVRDRDTMEQVRIPIA
                     ELKDYFADKFMF"
     gene            1736..3232
                     /locus_tag="ACZPCL_03005"
     CDS             1736..3232
                     /locus_tag="ACZPCL_03005"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1161488.1"
                     /translation="MKLKTRLAVAFMIITIVPMLLIFVSFFAINDHQARSFTKEYGLT
                     EQVDLLNGNSTQVFNRLTRRVQQVIREELDKKPELYEDSSHLDEVNAELQKHYAYLVV
                     RKGTDIIYFGDADEENKTQLCEQLPEFDILQGDLEGGIYLDGESQHLVKQMDFQFPDK
                     TEGSVFIISNVDDLLPEVKSMIREMLFLGVMILLIAGITLTFWVYQSILSPLNKLQEA
                     TKKIRDGNLEFTLDVDADDEIGHLCQDFEEMRMRLKESAEEKVQYDKESKELISNISH
                     DLKTPITAIKGYVEGIMDGVASSPEKLDKYIRTIYNKANDMDRLIDELTFYSKIDTNK
                     IPYTFSKINVSQYFRDCVEEVGLDMEARGIELGYFNYVDEDVVVIADAEQMKRVINNI
                     ISNSVKYLDKKKGIINIRIKDDGDFIQVEIEDNGKGIAAKDLPNIFDRFYRTDSSRNS
                     SQGGSGIGLSIVRKIIEDHGGRIWATSKEGIGTEVHFVLRKYQEVVQE"
     gene            3229..3918
                     /locus_tag="ACZPCL_03010"
     CDS             3229..3918
                     /locus_tag="ACZPCL_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1161489.1"
                     /translation="MSKILIVEDEESIAELEKDYLELSGFEVEIENNGTDGLERALKE
                     EYDLLILDLMLPGTDGFEICRRVREVKNTPIIMVSAKKEDIDKIRGLGLGADDYITKP
                     FSPSEMVARVKAHMARYERLVGSGQPANEIIEIRGLKIDKTARRVWVNGEEKTFTTKE
                     FDLLSFLAQNPNHVFTKEELFSKIWDMESIGDIATVTVHIKKIREKIEFNTAKPQYIE
                     TIWGVGYRFKI"
     gene            4333..6957
                     /gene="ppdK"
                     /locus_tag="ACZPCL_03015"
     CDS             4333..6957
                     /gene="ppdK"
                     /locus_tag="ACZPCL_03015"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR1161490.1"
                     /translation="MATKWVYLFSEGNANMRNLLGGKGANLAEMTNLGLPVPQGFTIT
                     TEACTQYYEDGRKINDEIMGQIMEYIGKMEEITGKKFGDKENPLLVSVRSGARASMPG
                     MMDTILNLGLNEEVVEVLAKKSGNPRWAWDCYRRFIQMFSDVVMEVGKKYFEELIDKM
                     KAEKGVKQDVELTAEDLHDLAEQFKAEYKEKIGKDFPTDPKEQLMEAIKAVFRSWDNP
                     RANVYRRDNDIPYSWGTAVNVQMMAFGNMGETSGTGVAFTRDPATGEKHLMGEFLMNA
                     QGEDVVAGVRTPQKIDQLKEVMPEVYEQFVGICHTLEDHYRDMQDMEFTIEDRKLYML
                     QTRNGKRTAKAALKIACDLVDEGMIDEKKAVKMIDPRNLDTLLHPQFDAAALKKAEPV
                     AKALAASPGAACGKIVFTAEDAKTWKAKGEKVVLVRLETSPEDIEGMKAAQGILTVRG
                     GMTSHAAVVARGMGTCCVSGCSDIIMDEANKKFVLAGKEYHEGDWLSIDGSTGKIYDG
                     IIPTVDATIAGEFGRIMAWADKYRRLQVRTNADTPRDAAKARELGAEGIGLCRTEHMF
                     FEGNRIDAFREMICSETVEEREAALEKILPEQQGDFEKLYEALEGNPVTIRFLDPPLH
                     EFVPTEEEDIKKLADAQGKTVEQIKAICDALHEFNPMMGHRGCRLAVTYPEIAKMQTK
                     AVIRAAINVKKAHADWNVCPEIMIPLICDTKELKYVKKIVVETADAEIAAAGVDLKYE
                     VGTMIEIPRAALTADEIAKDAEFFCFGTNDLTQMTYGFSRDDAGKFLNAYYDAKIFEN
                     DPFAKLDQTGVGKLMEMAIKLGKATRPELHVGICGEHGGDPSSVEFCHKIGLDYVSCS
                     PFRVPIARLAAAQAALAD"
     gene            7225..7959
                     /locus_tag="ACZPCL_03020"
     CDS             7225..7959
                     /locus_tag="ACZPCL_03020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1161491.1"
                     /translation="MKKWMKICLMAAASAMLLAGCGTKDNTAETTAVETTAKAEDTKE
                     TETEKAEETTRAAASETAETTEAAEENSELDLAAAAGKHHVEITVKDYGTINVELDGD
                     AAPITVANFLDLAGNGFYDGLTFHRIISGFMIQGGDPLGTGMGGSEREIKGEFDSNGV
                     KNPLSHTRGAISMARAQRKDSASSQFFIVHEDSTFLDGEYACFGYVTDGMDVVDAICE
                     DTKVEDNNGTVAKENQPVIESIKVID"
     gene            8004..9437
                     /locus_tag="ACZPCL_03025"
     CDS             8004..9437
                     /locus_tag="ACZPCL_03025"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR1161492.1"
                     /translation="MRKNVGKKKLLLTVLSALTLSLAATIGAFADESDTSKLVSGTKI
                     NGVGVGGLTPDEAKARIEGFYAGEYTLNIKEKGGKEESIKGSDIGYQVTVSGSIQNIL
                     DSQNATGRVAGPSGENTHTMEVSAQYNEEALNNKISNLSCISGGSIVTTKDASISAYE
                     EGKEFTIVPAVQGNDVDPEKTKQVITAVVRAGSRELSLEETGCYRTVGVWESDENLKA
                     LCAAMNSRRTKQLRYVFGDASEVLSGETMASWITGSSNGQVTLDQEKVAAFVANLAAT
                     YDTAGKTRTFTGVTGAEYQLTGPYGWKIDQAGEIAALTELIQSGSAWQDGDSADREPV
                     YSQSAVSRTGGDWGNTYVQVDLGGQHVYMVKDGTVVWDAPCVTGNVSKDYTTPAGIYS
                     LTYKEKDRILRGKKLDNGKYEYESHVDYWMPFNGGIGLHDASWRSKFGGTIYQTSGSH
                     GCVNLPPASAATLYDLLYKGIPVICTP"
     gene            complement(9675..10940)
                     /locus_tag="ACZPCL_03030"
     CDS             complement(9675..10940)
                     /locus_tag="ACZPCL_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HIR1161493.1"
                     /translation="MMPSSSEQRPTIHVQTLGGFSIRTNGCQINDNSNQSKKPWALLE
                     YLVIFQKKDINPAELIRIIWADDPGVNPNGALKTLMFRSRKLLEPLGISPQKLLVQQR
                     GTYAWTQDYPTVLDIDEFESVCNRALSNSLDPEENLSLCMEGLSIYKGDFLPKSEYES
                     WVIPISAYYHSLYQKLVYKTIQLLLDKQAYGQITSICQTAIGIEPFDEEFHYHLVYSL
                     YKDGHTSQALEEYSNTLDLFYNEFSISPTEHFKELYNIIRNSERGINTNLDSIQTSLN
                     EESSGGAFYCEYPIFRDLFQLERRSIQRTGDSIYLCLLTVTGIDGKTPKSMILQKAME
                     HLNTSIMASLRCSDVFTRYSISQYLILLPTVTIEKGEMVLKRIISNFRKLYNRKDITV
                     EIRLQPVLPWKNTPLGETLNYEELTEEEP"
     gene            11139..12008
                     /locus_tag="ACZPCL_03035"
     CDS             11139..12008
                     /locus_tag="ACZPCL_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161494.1"
                     /translation="MSVAKTGAIPNSYRQIHIAVDDFENRILKGRINHESKEYGIVFY
                     SLSEMVFALEELFEEIHYPMKSVDHRNFSKKEYLDLKEESREVRIEGLKSETRGKLAD
                     FCLRVQYRYYASWQGQIEQIGTNAKRHFDSFMELMDFLDQQLGEGVTRLPYGLGKKMC
                     EVTVNNYEGHVMSGDVSHPAVEDRRAFYNEFELREKIAEMIDPLPEQEKEPAVIISRS
                     FRVENGNYSPATFVVRVLFRRNSTWQGTVGWKEKRCQVSFRSFMELLLLMHEAVARSG
                     QWEKEPVKKTHTA"
     gene            12048..12950
                     /gene="srtB"
                     /locus_tag="ACZPCL_03040"
     CDS             12048..12950
                     /gene="srtB"
                     /locus_tag="ACZPCL_03040"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HIR1161495.1"
                     /translation="MEDSKKSGRDTLWKILVCVALLVFLVSGGLTLRSYLADKRAQEE
                     YERLAREAAQTTQETITIEETEKQELETEPEKEEYQSPYDFDTLKKENPDTIGWIHIP
                     DTKISYPIVQGTDNDFYLKHDFNGEASVAGSIYLDYESEGDFEGRNNILYGHNMKNGS
                     MFKDIVRYKDPSYFKEHQYFSIYTPEREIRLKAVSCYYGEAKPIVRKTRFKSQEAFDE
                     FVKEMIAPCSYAETVEYPVKALYTFVTCSYEINDARTFLFAVEADEQGNEITADEEYQ
                     QKMTDLMIKKAEEAEAVSKEAGKK"
     gene            13233..14156
                     /locus_tag="ACZPCL_03045"
     CDS             13233..14156
                     /locus_tag="ACZPCL_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1161496.1"
                     /translation="MKRNVMRTMSLAFAGIMAAGALTACGGNNTAATTAAPAEQTSAA
                     GESAAEEATAASGEKKVLKVAMECAYAPYNWTQPDDSNGAVPINDSNEYAYGYDVMMA
                     KKICDELGYDLQIVRLDWDSLIPAVSTGQVDCVIAGQSITTERLQAVDFTEPYYYATI
                     VTLVKNGSKYADAKSVADLAGATCTSQQSTIWYDTCLPQIQDANILAATASAPDMLMS
                     LNADKCDLVVTDQPTGKGALVAYPDFKLLEFGGGEDDFQVTDEDINIGISLKKGNTEL
                     KEAIDSVLSKMTKDDFSAMMDEAISVQPLAN"
     gene            14182..14973
                     /locus_tag="ACZPCL_03050"
     CDS             14182..14973
                     /locus_tag="ACZPCL_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1161497.1"
                     /translation="MPTDFLGRVMVVFNRYGMSMLQGAGVSLKIALVGTLVGCLIGFA
                     VGIVQTIPSEKGDNPLKKGALWIIRLILNAYVEFFRGTPMMAQAMFIYYGLMPLLGIN
                     STITQAAYFILSINTGAYMAETVRGGILSIDPGQTEGAKSIGMTHVQTMIYVILPQAL
                     RNLMPQIGNNLIINIKDSCVLSVIGVAELLYKTKAAAGALYMNFETYTITMIMYFIMT
                     FTCSRLLRLWEKKMDGADNFDLATTDTLAYTSGMYRYPDDKKEEQ"
     gene            14973..15716
                     /locus_tag="ACZPCL_03055"
     CDS             14973..15716
                     /locus_tag="ACZPCL_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1161498.1"
                     /translation="MSEKILEIRHLSKTFGTNLVLKDIDFTVNSKDVTCIIGASGSGK
                     STLLRCLNLLETPTTGEILYHGNNIADAKVNAPKYRSRVGMVFQSFNLFNNMTVLENC
                     MVGQVKVLKKGKEESKQKAMYYLEKVGMAPYINAKPRQLSGGQKQRVAIARALAMEPE
                     VLLFDEPTSALDPQMVGEVLEVMRKLAKEGLTMIIVTHEMAFARDVSTHVVYMANGVI
                     VEEGTPADIFANPQREQTMEFLGRFMHEK"
     gene            complement(15847..16692)
                     /locus_tag="ACZPCL_03060"
     CDS             complement(15847..16692)
                     /locus_tag="ACZPCL_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1161499.1"
                     /translation="MKEKRLAGAALYHIIIFAIGIIMVYPLIWMVMSSFKETNTIFTT
                     ASSLIPEHFVTDNYANGWKGFAKISFSTFFKNSIFISVIATIGTIASSAVVAYGFARF
                     KFRGKGILFSAMLLSMMLPAQVLMIPQYLWYQKLGWVGSFLPLTVPYFFAIQGFFVYL
                     LSNFIGGIPIELDEAAKIDGCSYWGIFTRIMIPLIKPAVVTGCIFSFMWRWDDFLSAL
                     LYINKTAMYPVSLALKLFCDPGSSSDYGAMFAMASLSILPSVLIFIFFQKYLVEGIST
                     SGLKG"
     gene            complement(16695..17588)
                     /locus_tag="ACZPCL_03065"
     CDS             complement(16695..17588)
                     /locus_tag="ACZPCL_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1161500.1"
                     /translation="MKTIRSLFNREDTAGIMFTLPFTLGFLFFMIVPMGISLYYSFCK
                     YNILSPPVFTGLDNFKNMIADGTFLQTIKVTFFFAFVSVPLKLIFALLVAMLLLKNTK
                     MSGIYRAAYYLPSIIGGSVAVAILWKRMFAMDGVVNKLLGSIGIKTSISWLGDTKTAI
                     WILILLAVWQFGSSMLIFLSSLKQIPTSLYEAAMVDGANPVSRFFKITLPLLTPTIFF
                     NLVMQMINGFLAFTQCYIITQGKPMNSTLLYTVYMYQQSFEFYNTGYGAAMAWVMLLM
                     IGLITLFLFATKRFWVYEGGL"
     gene            complement(17718..19088)
                     /locus_tag="ACZPCL_03070"
     CDS             complement(17718..19088)
                     /locus_tag="ACZPCL_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1161501.1"
                     /translation="MKKKLAVLLTAALAAASLTACGGSSSSGSSDTSAADTNTSQSAQ
                     SDGGSSDSSSLVMAWWGNQTRNERTQKILDMYSEENPGVTIDGQFSEFNDYWNKLATA
                     AAGHSMPDIVQMDYKYLQQYVNNNLLVDLTPYIEDGTIDTASCNQDVLNSGKVGDGLY
                     ALCNGINAPALLYNKTLLDENGITVKDNMTMDEFIALSREIEEKTGYKTNLCYNQNEQ
                     FLEYFLRADDIVLYQDGKMGGESAEPYVNFFKLYEDGIKDGWVVDPSVFAERTIGSVE
                     QDPLVYGSSPETMSWCSFSYSNQLTAIKNAAPEGTEIGITTWPSADPEKSDYLKPSQF
                     FAISADSKNPAEAAKILNYITNSADCNNVLLAERGIPLSSDVAEAISSNLDESSQQVI
                     DFIYNVVDPHSSQVNPPSADGSSEVNDLINKLEEQVCYGQLSAEDAGAQLFEQGNSIM
                     ASKASK"
     gene            19314..21095
                     /locus_tag="ACZPCL_03075"
     CDS             19314..21095
                     /locus_tag="ACZPCL_03075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing sensor histidine kinase"
                     /protein_id="HIR1161502.1"
                     /translation="MKKNIRILIGRFRHWSLKYKILTIVLFNSVTMLLCAVFGYRLYT
                     NAYNKLLFDSMASRLSVTSAQISQKMENAEYISNLILGSSSIQDCLSDLPSPEDAPAI
                     TEYNRTINNALTEYATLFRSNGIAYAAVYSKNGEYVNCTNWVTLKRTPGFLLDAAIQN
                     GYEKEGAVTWTHATRQPYMLLSRNVRQIRNMSLEPLGNMVIGLDLEQVVSEAVRFASQ
                     YKDQKVVIANQSGSLIYASSSLTDQRAEELITDPFTTYKIVSYDGHKYFAVKGTLPHY
                     QYSYVTLAPFDEVAGALTVSMQTTFMIFLVGILFIWYLSSRLIRGIIRQFDALIQKMK
                     LFTQNELELEPEEEDYGSQPNEIGALHHQFHQMALRIQNLVKVNYVNEILAKDAQLKA
                     LRSQISPHFLYNTLETINWRAKAMGNEVISQMVESLGNLLRVSLSSQKTLVTLAYELE
                     LVDSYITIQKIRFEERLEFKVITDEKAKEGMIPPLTIQPLVENAIHYGMEEMTEVCHI
                     YLQAEVKEGVLTIQVRNEGSNFEEELLEKLKNKSRSPHGFGIGLLNIDQRIRLLFGEE
                     YGLTLSNEHDFAVATIKMPFRKEEETC"
     gene            21089..21211
                     /locus_tag="ACZPCL_03080"
     CDS             21089..21211
                     /locus_tag="ACZPCL_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161503.1"
                     /translation="MLKLLIADDERIIRETIFNIIDWKKHDIEVIGLCKNGIEA"
     gene            21215..22594
                     /locus_tag="ACZPCL_03085"
     CDS             21215..22594
                     /locus_tag="ACZPCL_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1161504.1"
                     /translation="MILDESPDIVLTDIRMPGMGGLELIRKIRQTDLQIQFIILSGYG
                     EFEYAKEAMSYGIRHYLLKPCNETQILECIEQCRKDHEKQARERKMLQQQFVLYDGMF
                     HSVLSSIINDCLDEKMTLEEIISPYEQYLDFHFAAYRLFYVYYLELQGLPSFLQQLKA
                     YMEEKMPQTILYGMYVKNTLVLFMQNAGKACDDLHVFLQQLSPEGQKITTEIREENYS
                     SLKTLLTQVTGKLLRYSMIYYINHFHITAICNYNWVIDQIRVLYQQIQEGNREKTDQA
                     VELLNSIDNIDFLKQLAGNLFLKMASDFQTFSTVDLAQWLTKMEKEEDLDPLRTMISD
                     KFRDLVSLKEGEETVSPMVSQIYVYVEQHLQDSALTLKWIAENYLFMNVDYVSKKFYK
                     ETGQKFSQYLTKMRIGKAKELIAKNPEEPVKNVAEQVGCGNNPQYFSQLFKKQTGMTP
                     TDYITSLGH"
BASE COUNT         6916 a         4380 c         5671 g         5851 t
ORIGIN      
        1 cttcatccag aggttgcaat agcataaagc actattttgt tagctgaaat attgaatttt
       61 caaggtgcga agccctgttt aggtgtggga agttttagta agtaaaaatg gcagtttgca
      121 gatgagtggt ccacggatct gcagaatgct tgtaaagaga gaggatactg atatggaaaa
      181 gacaatggaa aaaatcgtag ctcttgcaaa aaacagaggt tttgtatatc caggttctga
      241 gatctatggc ggtcttgcaa atacctggga ttacggtaat ctgggcgttg agctgaagaa
      301 caacgtaaag aaagcctggt ggcagaaatt cgtacaggaa agcccttata acgtaggtgt
      361 tgactgtgcc atcttaatga actcccagac atgggtagcc agcggacatc tgggcggttt
      421 ctccgatcct ctgatggact gtaagcagtg taaagaacgt ttccgcgctg acaagctgat
      481 cgaagattat aacgatgagc atggcattga gatcgaaggt tctgtagacg gctggtccca
      541 ggaacagatg aagcagtaca tcgaagacaa gcatatctgc tgcccaagct gtggtgctca
      601 cgactttaca gatatccgtc agttcaacct gatgttcaag acattccagg gtgtaaccga
      661 ggatgctaag aatactgtat accttcgtcc agagactgct cagggtattt tcgtaaactt
      721 caagaatgta cagagaactt ccagaaagaa agttccattt ggtatcggac agatcggtaa
      781 gtccttccgt aacgagatca cacctggtaa ctttactttc cgtaccagag agtttgagca
      841 gatggagctt gaattcttct gcaaaccagg tactgacctt gagtggtttg cttactggaa
      901 acagtactgc atcgactggc tgcagaaatt aggcattaag ccagatgaaa tgcgtgcaag
      961 agaccattct ccagaagaac tgtgcttcta cagcaaggct actactgacc tggaattcca
     1021 gttcccattt ggctggggcg agctttgggg tattgcagac agaactgact atgatctgac
     1081 ccagcatcag actgtatccg gacaggatat gacttacttt gatgatgaga gcaaggaaaa
     1141 atatatccca tatgtaatcg agccttcact gggtgctgac cgtgtaacct tagcattcct
     1201 gtgtgcagca tatgacgaag aagagatcgg agaaggtgat gtaagaactg tccttcactt
     1261 ccatccggct ctggcacctg taaaggtagg cgttctgcca ctgtccaaga agttaaacga
     1321 aggagcagaa aaggtatacg ctgaactgag caagtacttc aactgcgaat tcgatgacag
     1381 aggaaacatc ggtaagcgtt acagaagaca ggatgagatc ggtactccat tctgcgtaac
     1441 ctatgacttt gactctgaag aagaccatgc agtaactgtc cgtgacagag acaccatgga
     1501 gcaggttcgt atcccgatcg ctgagttaaa ggattacttt gctgataagt ttatgttcta
     1561 attgagattt aagttaaact ttaaattaaa aatgcccgtt ttcctgtttt ggggaagcgg
     1621 gcattttgct atgtatattg agaacgcgcc agtgacgcgt tcgctgattt tagaatgatt
     1681 ttataaatga tagtgaggaa gtatgatata atgagtccat tggaggtcct tacttatgaa
     1741 actaaagacc cgcttagcgg ttgcatttat gatcatcacc attgttccca tgcttcttat
     1801 ttttgtgtcc ttttttgcca tcaatgatca tcaggcacgt tcctttacaa aagagtatgg
     1861 tctgacggaa caggtggatc ttttaaatgg taattctacc caggtattta accgtctgac
     1921 ccgccgggta cagcaggtga tccgggaaga actggataaa aagcctgaat tatatgaaga
     1981 cagcagtcat ttagatgagg tcaatgcgga acttcagaag cattatgctt atctggtagt
     2041 gcgaaaggga accgacatca tttattttgg tgatgcagac gaggaaaata agacccagct
     2101 gtgtgagcag ctgccggaat ttgatatcct tcagggagat ttagagggcg gtatctactt
     2161 agacggcgag agccagcatc tggtaaagca gatggatttt cagttcccgg ataagacaga
     2221 aggaagcgta tttattattt ctaatgtaga tgatctgctg ccggaagtaa aatccatgat
     2281 ccgggaaatg ctctttttag gtgttatgat ccttctgatc gccggaatta ccctgacttt
     2341 ctgggtgtac cagtctattt taagcccctt aaacaagctt caggaagcta ccaagaagat
     2401 ccgtgacggc aatctggaat ttaccctgga tgtggacgca gatgatgaaa tcggccatct
     2461 ttgccaggac tttgaagaaa tgcgtatgcg cttaaaagaa agtgcagagg aaaaagtcca
     2521 gtatgacaaa gaaagcaaag agctgatcag caatatttcc catgacttaa agacaccgat
     2581 cacagctatt aagggctatg tggaaggcat tatggacgga gtggcatctt caccggaaaa
     2641 gctggataaa tacatcagga ccatttataa taaagctaat gacatggacc gcctcatcga
     2701 tgagctgaca ttctattcca agatcgatac taacaagatc ccatatacat tctccaagat
     2761 caatgtttcc cagtatttca gggactgcgt ggaagaagtg ggactggata tggaagccag
     2821 aggtattgaa ctgggatatt ttaactatgt agatgaagac gtagtggtca ttgccgatgc
     2881 agagcagatg aagcgtgtga tcaacaatat catcagcaat tctgtgaaat atctggacaa
     2941 gaaaaaaggt attatcaata tccgcatcaa ggatgacggg gatttcatcc aggtggagat
     3001 cgaggacaat ggaaaaggaa ttgcagccaa agatctgcca aatatctttg accggttcta
     3061 ccgcaccgac tcttccagaa attcttccca gggcggaagc ggtatcggtc tgtccattgt
     3121 gcgaaagatc attgaggatc acggtggccg tatctgggct accagtaaag agggaatcgg
     3181 aacagaggtg cactttgttt taagaaaata tcaggaggtt gtgcaggaat gagtaaaata
     3241 ctgatcgtag aagatgagga aagcattgca gagctggaaa aggattatct ggagctgtca
     3301 ggctttgaag tagaaataga aaataatggt acagacggtt tagaacgggc attaaaagag
     3361 gaatatgacc tgctgatcct ggatctgatg cttccgggaa ccgatggttt tgagatctgc
     3421 cgccgggtac gtgaggtgaa aaatacccct attatcatgg tttccgcaaa gaaagaagat
     3481 attgacaaga tccgcggact gggactggga gctgacgatt acatcaccaa gccattcagt
     3541 ccaagtgaaa tggtagccag agtaaaagcc catatggcaa gatatgagcg cctggtagga
     3601 agcggccagc cagcaaatga gatcatcgag atccgcggtc ttaagatcga caagactgca
     3661 agaagagtgt gggtaaatgg ggaagaaaag acctttacaa ccaaggaatt tgacctgctt
     3721 tccttcctgg cacaaaatcc caaccatgta tttacaaaag aagagctttt ctccaaaatc
     3781 tgggatatgg aatccattgg agatattgca actgtaacag tgcatattaa aaagattcga
     3841 gagaagattg agtttaatac cgcaaagcct cagtatattg agactatctg gggagtgggg
     3901 tatcggttta agatataatg agtccaagag aaaaacaagc gccgctaaaa gcggcatttg
     3961 tttttcttgg accattaaca agcgttccgt ctgcgtcagc aggcggaact tttttctttc
     4021 cagacacatc aaagaagcgt ctggggcacg aataaatgct tttgtataca atgcacattc
     4081 gcaaatacga accaacaacg gatatgtgaa aatgacagaa agtggaagcg cttacactgg
     4141 tttttacagt aaaattaaca aattttttca aagttttgcc tccaaaaggg tttactgaaa
     4201 aaaactttgt gatataataa acacgttgga cggacgaagg gagtcattgg ttccgcccta
     4261 agttaagcta agtcggcaaa cgtcaggtga aaggggcctg gcggactgct atatcagagg
     4321 aggaaaagac ttatggcaac aaagtgggtt tacttgttca gtgaaggcaa tgcaaacatg
     4381 agaaatcttt taggaggtaa aggtgcaaac ctggctgaga tgacaaatct tggtttaccg
     4441 gttcctcagg gatttacaat tacaacagaa gcttgtacac agtactatga agatggcaga
     4501 aagatcaatg acgagatcat gggccagatc atggaatata tcggaaagat ggaagagatc
     4561 accggcaaga agtttggtga taaggaaaat ccacttctgg tttccgttcg ttccggtgct
     4621 agagcatcca tgcctggtat gatggatacc atcctgaact taggtctaaa tgaagaagta
     4681 gtagaggttc tggctaagaa gtccggcaat ccacgttggg cttgggactg ctacagaaga
     4741 tttatccaga tgttctccga tgttgttatg gaagtcggaa agaaatactt tgaagagctc
     4801 atcgacaaga tgaaagctga aaaaggcgtt aagcaggacg ttgaactgac tgcagaagat
     4861 ctgcatgatc tggctgaaca gttcaaggct gaatataaag agaagatcgg aaaagacttc
     4921 ccaactgatc ctaaggagca gttaatggaa gcaatcaagg ctgtattccg ttcatgggat
     4981 aacccaagag caaatgttta ccgtcgtgac aacgatatcc catattcctg gggtactgca
     5041 gtaaacgtac agatgatggc atttggtaac atgggtgaga cctccggtac tggtgttgca
     5101 tttacccgtg acccagcaac tggtgagaag cacttaatgg gcgagttcct gatgaatgca
     5161 cagggcgaag acgtagtagc tggtgtgcgt acacctcaga agatcgacca gttaaaggaa
     5221 gttatgccag aagtttatga gcagttcgta ggtatctgcc atactctgga agaccactat
     5281 agagatatgc aggatatgga gttcaccatt gaggacagaa agctgtacat gttacagacc
     5341 cgtaatggta agagaactgc aaaggctgca ttaaagatcg cctgcgactt agttgacgaa
     5401 ggcatgatcg atgagaagaa agcagttaag atgatcgatc cacgtaacct ggatacactg
     5461 cttcatcctc agtttgacgc tgctgcactt aagaaggctg agcctgtagc taaggcactg
     5521 gctgcttcac caggtgctgc ctgcggtaag atcgtattta cagcagaaga tgcaaagact
     5581 tggaaggcaa aaggcgagaa ggtagttctg gttcgtctgg agacctctcc tgaagatatt
     5641 gaaggtatga aggctgctca gggtatcctg actgtccgcg gtggtatgac cagccatgca
     5701 gcagtagttg cacgtggtat gggtacctgc tgtgtatccg gctgctccga tatcattatg
     5761 gacgaagcga acaagaagtt cgttctggct ggaaaagaat accatgaagg tgactggctg
     5821 tctattgatg gttctaccgg taagatctac gatggcatta tcccaactgt agatgctacc
     5881 atcgctggtg agttcggaag aatcatggca tgggctgaca aatatagaag attacaggtt
     5941 cgtaccaatg cagatactcc tagagatgct gcaaaggcta gagagcttgg cgcagaaggt
     6001 atcggtcttt gccgtacaga gcatatgttc tttgaaggca acagaattga tgcattccgt
     6061 gagatgatct gctctgaaac agtagaagaa agagaagcag ctctggagaa gatcttacct
     6121 gagcagcagg gagatttcga gaagctgtac gaagctctgg aaggcaaccc agttaccatc
     6181 cgtttcctgg atccgcctct gcatgagttc gttcctactg aggaagaaga tattaagaaa
     6241 ctggctgacg cacagggcaa gactgttgaa cagatcaaag ctatctgcga tgcacttcat
     6301 gagttcaacc caatgatggg acatcgtggc tgccgtctgg ctgttaccta tcctgagatc
     6361 gctaagatgc agacaaaggc tgttatccgt gcagctatca atgttaagaa ggctcatgca
     6421 gactggaacg tttgcccaga gatcatgatc cctctgatct gcgataccaa ggaattaaag
     6481 tatgttaaga agatcgttgt tgagaccgct gatgctgaga tcgcagcagc tggtgttgac
     6541 ctgaaatacg aagttggtac tatgatcgag atcccaagag cagctttaac tgctgatgag
     6601 atcgctaagg acgctgagtt cttctgcttc ggtactaacg acttaaccca gatgacttac
     6661 ggattctccc gtgatgatgc tggcaagttc ttaaacgctt actatgatgc taagatcttc
     6721 gagaatgatc catttgcaaa actggatcag actggcgtag gcaagctgat ggaaatggct
     6781 attaagttag gaaaggctac acgtcctgaa cttcatgtag gtatctgcgg cgagcacggc
     6841 ggtgacccaa gctccgtaga attctgccat aagatcggac tggactatgt atcctgctca
     6901 ccattccgtg taccgatcgc tcgtttagca gcagcacagg cagctctggc agactaattg
     6961 cagagattta aaacaggcta atcgattaaa aattgttata taagggataa aagtgacagc
     7021 gctgaaggaa acttcggcgc tgttgctttt ttgtatcttt cttaataaat cttacggaag
     7081 tttgatattt ttgtgaaacg ctttactgta tctggggaaa atgctatgat gaaaacaacg
     7141 agtgccccgc ctgccaaagc aggcatatcg cgtacaggca tgagtaaagc ttatgatagc
     7201 agaagaaagg aattgaaatt aagaatgaag aaatggatga agatctgcct gatggcagca
     7261 gcatctgcca tgttattagc aggctgcggt acaaaagata atacagcaga gacaacagca
     7321 gttgaaacaa ctgcaaaggc agaagataca aaagaaactg agacagaaaa agcagaagag
     7381 acaacccggg ctgcagcaag tgaaacagca gaaactacag aggcagcaga ggaaaacagt
     7441 gaactggatc tggcagcagc tgcgggaaag caccatgtag agatcactgt aaaagattac
     7501 ggcaccatta atgtagaatt agacggggat gcagcaccga tcacagttgc aaacttttta
     7561 gatctggctg gaaacggatt ttatgacgga cttaccttcc accgcatcat cagtggattt
     7621 atgatccagg gcggtgaccc attaggaaca ggtatgggcg gttccgagcg tgagatcaaa
     7681 ggcgaatttg actccaatgg cgtgaaaaat ccattatccc atacaagagg agctatttct
     7741 atggcaagag cccagcgtaa ggacagcgca agctcccagt tctttatcgt acatgaggac
     7801 agtaccttct tagacggtga atatgcctgc tttggttatg taacggacgg tatggatgtg
     7861 gttgacgcga tctgtgaaga tacaaaggta gaagacaaca acggaacagt tgcaaaagaa
     7921 aaccagccgg tgatcgaaag cattaaggtg atcgactgac aggggagttt acaggtcagg
     7981 tgctcaaaaa aggagtatgg agtatgagaa aaaatgtagg aaaaaagaaa ctgcttttaa
     8041 cggttctgtc agcactgaca ttgtcattag ctgcaaccat aggtgcattt gcagatgaaa
     8101 gtgatacctc aaagctggta tccggcacca agatcaatgg cgtaggtgta ggtggattga
     8161 cgccagatga agcaaaagca aggatcgaag gcttttatgc tggggaatat acactgaata
     8221 tcaaggaaaa aggcggaaaa gaagagagca tcaaaggcag cgatattggc taccaggtga
     8281 ctgtttccgg cagtatccag aatattttgg acagtcagaa tgccaccggt cgtgtagcag
     8341 gaccttccgg tgaaaatacg catacaatgg aagtttctgc ccagtataac gaagaggcgt
     8401 taaacaataa gatcagcaat ctttcctgca tcagtggcgg aagtattgtg acaacaaaag
     8461 atgcttccat ctctgcttat gaagaaggaa aagaatttac catcgttccg gctgtacagg
     8521 gaaatgatgt ggacccggaa aagacaaaac aggtgatcac agctgttgta agagccggaa
     8581 gcagggaact ttccctggaa gaaaccggct gttaccgtac cgttggtgtc tgggaaagtg
     8641 atgaaaacct gaaggcttta tgtgcagcta tgaacagccg caggaccaaa cagctgcggt
     8701 atgtatttgg agatgcgtca gaagtccttt ccggcgagac catggccagc tggatcacag
     8761 gaagcagcaa tggacaggta actctggatc aggaaaaagt cgcagcattt gtggcaaatc
     8821 tggcggcaac ttacgataca gcaggaaaga cacgtacctt tacaggggta acaggagcag
     8881 aataccagct tacaggccct tatggatgga agattgacca ggcaggcgag atcgcagccc
     8941 ttactgagct gatccagagt ggttcggcat ggcaggatgg tgacagtgca gacagggagc
     9001 ctgtatacag ccagagtgca gtttccagaa caggtggtga ctggggcaat acatatgtac
     9061 aggtggatct tggcggacag catgtataca tggtaaaaga tggtaccgtt gtctgggatg
     9121 cgccctgtgt aacaggaaat gtgtcaaagg attatacaac accggcaggt atctacagtc
     9181 tgacttataa agaaaaagac cgtatcctgc gtggaaagaa gctggataac ggcaaatatg
     9241 aatacgaaag ccatgtagat tactggatgc catttaacgg aggcatcgga cttcatgatg
     9301 cttcctggag aagcaaattc ggtggaacca tttatcagac cagcggcagc catggctgcg
     9361 taaatctgcc accggccagt gcagcaactt tatatgatct gctttacaag ggaataccgg
     9421 tgatctgcac gccataaata aagagatgac gggagcagaa accataaaaa agaatgtgcc
     9481 ttggcacatt ctcgcgccga gcgcggtcgc ttgcgacaaa cttcctcatc gcgcgagtgc
     9541 gcatcgttgg caggtcgtgc ctttttgtgt actagggagt tccaacggaa tttcctagta
     9601 cataaaataa aaacagatgg aaccgacctg gctccatctg ttttttaaat gttttttgtt
     9661 tacttacgga aattctaggg ttcttcttct gtcagttctt cgtaatttaa ggtttcgcct
     9721 aaaggcgtat ttttccaggg aagaaccggt tgcaaacgga tctcaactgt aatgtccttc
     9781 cggttgtaaa gcttgcggaa gttgctgata atacgtttta agaccatttc tcctttttca
     9841 atggttacag taggcagtaa gatcaggtac tggctgatac tgtaacgggt gaatacatca
     9901 ctgcagcgca gggaagccat aatggaagta tttaagtgtt ccattgcttt ttgaaggatc
     9961 atgctctttg gagtttttcc gtcgatccct gtaactgtca gcaggcagag ataaatagag
    10021 tctccggtac gctggataga acggcgttct aactggaaca gatccctgaa aataggatat
    10081 tcacagtaga aagccccgcc gctgctttcc tcgtttaagg aagtctggat agagtcaagg
    10141 tttgtattga tgccacgttc actgttacgg atgatattat acagctcctt aaaatgttcc
    10201 gttggtgaaa tggaaaattc attgtagaac aggtctaagg tattgctata ttcttcaagt
    10261 gcctggctgg tatggccgtc cttatataag gaatagacca ggtgataatg aaattcctca
    10321 tcaaaaggct cgatcccaat agcagtctgg cagatggaag tgatctgtcc gtaagcctgt
    10381 ttgtccaaaa gcagctgaat ggtcttgtaa accagcttct ggtacagaga gtgataataa
    10441 gcactgatgg gaatgaccca tgattcgtat tctgattttg gaaggaaatc acccttgtaa
    10501 atagaaagac cctccataca cagggacaga ttttcttccg gatcaaggga gttgcttaaa
    10561 gcccggttgc atacagactc aaattcatca atatctaaaa ccgtaggata atcctgggtc
    10621 caggcatatg ttccccgctg ctggactaac agtttctgcg gggagatccc taacggctct
    10681 aacagtttac gggaacggaa cataagggtt tttaatgctc cgtttggatt gacaccggga
    10741 tcatcggccc agatgatacg gatcagttca gcagggttga tatccttttt ctggaatata
    10801 acaagatact ctaataatgc ccagggcttt tttgactgat tgctgttgtc attgatctgg
    10861 cagccgtttg tgcggataga aaaaccgcca agggtctgta catggatcgt tggtctctgt
    10921 tcagaagaag atggcataat ggcaggtctc ctttatatat catttttata tcgtgcattt
    10981 gaatttatac aataagtata actatacgct atggaaatgt ccagtttatt aagacgaaaa
    11041 aacgaaaaaa ttaagtattt tgtaaccgat ttgtaaccgg aaactgctat actgaaaata
    11101 gtataaaaca gaataataaa cagaaagtgg gtgaaaaaat gtcagtagca aagactggag
    11161 caataccgaa ttcttatcgt cagatccata ttgcagtgga tgattttgaa aacaggatat
    11221 taaaaggaag gatcaatcat gaaagtaagg agtatggcat tgttttttac agcctttctg
    11281 aaatggtgtt tgctttagag gagctgtttg aggagatcca ttaccctatg aaaagtgtgg
    11341 accaccgcaa tttcagcaaa aaagaatatc tggatctgaa agaggaaagc agggaagtaa
    11401 gaatagaagg attaaagtct gaaacaagag gaaaacttgc ggatttttgc ctgcgggtcc
    11461 agtatcgtta ttatgcttcc tggcaggggc agatcgaaca gatcggaacg aacgccaaaa
    11521 gacattttga cagctttatg gaactgatgg actttttaga ccagcagtta ggggaaggtg
    11581 taacaagact tccctatgga cttggcaaaa agatgtgtga agtcactgta aacaattatg
    11641 aaggtcatgt gatgagcggg gatgtgtccc atccggcagt agaagacaga cgggcttttt
    11701 ataatgaatt tgagctgagg gaaaagatcg cggaaatgat cgatcctctt ccggaacagg
    11761 aaaaagaacc ggcagtgatc atttcacgca gtttccgtgt ggaaaatgga aattatagtc
    11821 cggcaacctt tgtagtccgg gtgctgtttc gcaggaattc tacctggcag ggaacggttg
    11881 gctggaagga aaaacgctgc caggtaagct tcagaagctt tatggaactg ctccttttaa
    11941 tgcatgaggc tgtggcaaga tccggacagt gggagaaaga gcctgtaaag aagacacata
    12001 cagcatgatc ttttaaggaa aacatcttgg aaaggacaga atgatgtatg gaagacagta
    12061 aaaaaagcgg cagagatact ctgtggaaaa tactggtctg cgttgcactt ctggtatttt
    12121 tagtaagcgg cggcctgact ttgcgcagtt atcttgcaga taaaagagcc caggaagagt
    12181 atgaacgcct tgcaagggaa gcagcccaga cgacccagga gaccattaca atagaagaaa
    12241 cagaaaaaca ggaactggag acagaacctg aaaaagagga ataccagtca ccctatgatt
    12301 ttgatacatt aaaaaaggaa aatcctgata ccattggctg gatccatatt ccagatacaa
    12361 agatcagtta tcctattgta cagggaacag ataatgattt ttatctgaaa catgatttta
    12421 acggggaagc aagtgtagcc ggaagtattt atctggacta cgaaagcgaa ggtgactttg
    12481 agggaagaaa taatatcctt tacggccaca atatgaagaa cggaagcatg tttaaagata
    12541 ttgtccgcta taaagaccct tcttatttta aagagcatca gtatttttct atttatactc
    12601 ctgaacggga gatacgttta aaggcggtat cctgctatta tggagaggca aagcccattg
    12661 tgcgcaagac ccgttttaag agtcaggaag cttttgatga gtttgtaaaa gagatgatcg
    12721 ccccttgctc ctatgcggaa accgtagaat acccggtgaa agcgctgtat acatttgtaa
    12781 cctgcagcta tgagatcaat gatgcaagaa ctttcttgtt cgctgtggag gcagatgaac
    12841 agggaaatga gatcacggcg gatgaagagt atcagcagaa aatgacagac ctgatgatta
    12901 aaaaggcaga ggaagcagag gcagtttcta aggaagctgg gaaaaagtag gaaaatatag
    12961 tggaaaatat ggcctgttaa aataaaaatt actattggaa aagcggagca gttatgctat
    13021 actaagcggg aaaatgctga tttatataaa aatcagcagt caggaaggct gtttcgcgaa
    13081 agaagccaag gaaaacgaag aagtcatacc tggtaacagg tatggctttc atttatggtg
    13141 atgttaccgc caggtgacat gtctgtttgt tataaggaca ggtatttttt gataacagac
    13201 tttcgaaata caggcaaaag gagagaacaa ttatgaaaag aaatgtaatg agaacaatga
    13261 gcctggcatt tgctggaata atggcagcag gtgcattaac agcatgtggc ggaaataata
    13321 cagcagctac tacagcagct ccggcagagc agacaagcgc agcaggagaa agcgcagctg
    13381 aagaggctac agcagcttcc ggcgaaaaga aggttttaaa ggtagctatg gagtgtgcat
    13441 acgcaccata caactggaca cagccagatg attccaacgg cgctgtacca attaatgaca
    13501 gcaacgaata tgcttatgga tacgatgtta tgatggcaaa gaagatctgt gatgagttag
    13561 gatatgacct gcagatcgtt cgtcttgact gggattcact gatccctgct gtaagcactg
    13621 gacaggttga ctgcgttatt gccggtcagt ccattactac agagcgtctt caggcagttg
    13681 attttactga gccatattac tatgcaacca tcgttaccct ggttaagaac ggcagtaagt
    13741 acgctgacgc taagagcgta gctgacctgg ctggtgcaac ctgtacttcc cagcagagca
    13801 ccatctggta tgatacctgc cttccacaga tccaggatgc aaatatctta gctgcaacag
    13861 caagtgcacc agatatgctg atgtccttaa atgctgacaa gtgtgatttg gttgtaactg
    13921 accagccaac cggtaagggt gctttagttg cttatccaga tttcaagctt cttgaatttg
    13981 gcggcggcga agatgatttc caggtaactg acgaggatat caacattggt atttccttaa
    14041 agaagggcaa tactgagtta aaggaagcta ttgacagtgt tctgtccaag atgacaaaag
    14101 atgatttctc tgcaatgatg gatgaggcaa tttccgtaca gcctctggca aactaattga
    14161 agagataaaa aggagaaagt tatgccaaca gattttttgg gccgtgtaat ggtcgtattc
    14221 aaccgctacg gcatgtctat gctgcagggt gcaggcgtca gcttaaagat cgctttagta
    14281 ggtacgctgg taggatgcct catcggcttt gccgtaggta ttgtccagac cattccttct
    14341 gaaaagggag acaatccttt aaagaaggga gctttgtgga tcatacggct gatcttaaat
    14401 gcttatgtgg aatttttcag gggtacacct atgatggccc aggctatgtt tatctactat
    14461 ggccttatgc ctttgctggg aattaattct actattaccc aggctgctta ttttatctta
    14521 tctattaata caggtgccta tatggcagag actgtacgag gcggtatcct ttctattgac
    14581 ccgggacaga ctgaaggagc aaaatccatt ggtatgaccc atgttcagac tatgatctac
    14641 gttatcctgc ctcaggctct gcgtaacctg atgcctcaga tcggcaacaa cctgatcatc
    14701 aatatcaagg acagctgcgt gctttctgtt attggtgtgg cagaactgct gtataagaca
    14761 aaggcagctg caggtgccct ttacatgaac tttgagacct ataccattac catgatcatg
    14821 tattttatca tgacatttac ctgctccaga ctgctgcgtc tgtgggagaa gaagatggat
    14881 ggtgcagaca attttgacct ggcaaccaca gatacactgg cttataccag tggtatgtac
    14941 cgttatccgg atgataagaa ggaggagcag taatgagcga aaagattttg gagatccgcc
    15001 atttaagcaa gacctttggc accaatctgg tcttaaagga tattgatttt acagtaaaca
    15061 gcaaggatgt tacctgtatc atcggagctt ccggttccgg taagtccacc ttgcttcgct
    15121 gcttaaactt actggaaaca ccgactaccg gcgagatttt atatcatgga aataacattg
    15181 ccgatgcaaa ggtaaatgca ccgaaatacc gttccagagt tggtatggta ttccagagct
    15241 ttaacctgtt caacaacatg accgttcttg aaaactgcat ggtgggacag gtcaaggtgt
    15301 taaagaaggg taaggaagag tcaaagcaga aggccatgta ttacctggaa aaagttggca
    15361 tggctcctta tatcaatgca aaaccaagac agctttccgg cggtcagaaa cagcgtgtag
    15421 ccattgcaag agccctggct atggagcctg aggtactgct gtttgatgag cccacatccg
    15481 cgctggatcc ccagatggtt ggagaggttc tggaagttat gcgcaagctt gcaaaagaag
    15541 gtctgaccat gatcattgtt actcatgaga tggcatttgc aagagatgta tctacccatg
    15601 ttgtatacat ggcaaacggt gtcatcgtag aagagggaac accggctgat atttttgcaa
    15661 atcctcaaag agagcagacg atggaattcc ttggaagatt tatgcatgag aaataaaaag
    15721 aatgagaaaa aagcctatgc aggctgcctg aatggcagct gcatgggctt ttttatgtat
    15781 tatacatatc ccgaatgcgc caacagcgta tttgctggtt gaaatggaaa tgcttatctg
    15841 tttggtttaa cccttcagac cagaggtact aattccttct accaggtatt tctggaagaa
    15901 aatgaagatc agtacggatg gaaggatgga aaggcttgcc attgcgaaca tagcgccgta
    15961 gtcggaagaa gaacctgggt cgcagaacag ttttaatgcc aggctgaccg gatacatggc
    16021 ggttttgttg atataaagca gggcggaaag gaaatcatcc catctccaca taaaggagaa
    16081 gatgcagccg gttacaactg ccggtttgat cagagggatc atgatccttg taaagatgcc
    16141 ccagtaggaa cagccgtcga tctttgctgc ttcgtccagt tcgatcggaa taccgccgat
    16201 gaagttggaa agcaggtata cgaaaaatcc ctggatcgcg aagaagtatg ggacagtcag
    16261 gggaaggaaa gaaccgaccc agcctaattt ctggtaccac agatactgcg ggatcataag
    16321 aacctgtgca ggaagcatca tggataacag cattgcggaa aataaaattc cttttccacg
    16381 gaacttaaat cttgcgaaac cgtaggctac aacagcagag gaagcaatgg ttccaatggt
    16441 agcgatcacg gagatgaaga tagagttttt aaagaaagtg gagaaagaga tctttgcaaa
    16501 tcctttccag ccgtttgcgt agttatccgt tacaaaatgt tccgggatca gggaactggc
    16561 ggttgtgaaa atggtgttgg tctccttaaa ggagctcata accatccaga tcagaggata
    16621 gaccatgatg ataccaatgg caaatatgat gatgtggtag agcgcagctc ctgccagacg
    16681 tttttccttc atgtctacag tcctccttca tatacccaga accgcttggt tgcaaacagg
    16741 aacagggtga tcagtccgat catcagcagc atgacccaag ccatggcagc gccataacca
    16801 gtgttgtaaa actcaaatga ctgctgatac atataaacgg tgtaaagcaa agtggagttc
    16861 attggcttac cctgtgtaat gatgtagcac tgggtaaatg ccagaaatcc gttgatcatc
    16921 tgcataacca ggttaaagaa aatagttggt gtcagcagag gaagtgtgat cttaaagaaa
    16981 cgggatactg ggttggcacc gtctaccatg gctgcctcat aaagagaagt cgggatctgc
    17041 tttaaagaag ataagaagat cagcatggaa gaaccaaact gccatacagc taacaggatc
    17101 aggatccaga tggctgtttt tgtatcacct aaccaggaaa tactggtttt aataccaata
    17161 gaacctaaaa gtttatttac aacaccgtcc atagcgaaca tacgcttcca aaggatagcg
    17221 actgctacag aacctccgat aatggaaggc agatagtaag ctgctctgta aataccggac
    17281 attttggtgt tttttaacag gagcattgca accagtaaag cgaagatcag ctttaaagga
    17341 acagatacaa aagcaaagaa gaaagtaact ttgatggtct gtaaaaaagt accatcagcg
    17401 atcatatttt taaagttatc aagacctgta aaaacaggcg gggacaaaat attatatttg
    17461 caaaaggaat aataaaggga aatgcccatg ggaacgatca taaagaagag aaagcccagg
    17521 gtaaagggaa gagtaaacat gatgcctgct gtatcttccc ggttaaacaa gctgcgaatg
    17581 gttttcatgt caaacctcct gtttttggat gtcttgggat aaaagatagt ttacaatggt
    17641 tcagggcggg aggaccgccc tgaattataa aaggatatgg taattaaaaa gagagtaatc
    17701 aataaaagta gatacgttta cttggaagct ttggaagcca taatggagtt gccctgctca
    17761 aagagctgtg cgcctgcatc ttctgcggac agctgtccgt agcatacctg ctcttccagt
    17821 ttattgatca gatcgtttac ttcactggaa ccatcagcac ttggtggatt tacctgtgaa
    17881 gagtgaggat ctacaacatt gtagatgaag tcaattacct gctggctgct ttcatccagg
    17941 ttagaagaaa tagcttctgc tacatcactg gacagcggga ttccacgttc agcaagaagt
    18001 acattgttgc agtctgcaga gtttgtgatg tagtttaaga tctttgctgc ttctgcagga
    18061 tttttggaat cagcagagat agcgaagaac tggcttggct ttaagtagtc agatttttca
    18121 ggatctgcag atggccaggt ggtgatacca atctcagtgc cttccggagc tgcgttcttg
    18181 atcgcagtta actggttgga gtaggagaaa gagcaccagg acatggtctc aggactggaa
    18241 ccatatacta atggatcctg ttcaacagaa ccgatggtac gctctgcaaa tacagaagga
    18301 tcaacgaccc agccatcttt aataccatct tcatacagtt tgaagaagtt tacatatggt
    18361 tcagcggact ctccgcccat ttttccatcc tggtacagaa caatgtcatc tgcacgaagg
    18421 aagtactcta agaactgctc gttctggttg tagcaaaggt tggtcttgta accggttttt
    18481 tcttcgattt ccctggaaag agcgatgaac tcatccatgg tcatgttgtc ttttacagtg
    18541 ataccgtttt catcaagtaa agtcttgttg tataacagag ctggtgcatt gataccattg
    18601 cacagtgcat ataagccatc tccaacctta ccggagttta aaacatcctg gttgcagctt
    18661 gctgtatcaa tggttccgtc ctcaatgtaa ggagtaagat caactaacag attgttgttt
    18721 acatactgct gtaaatattt gtagtccatc tgaacgatgt ctggcatgga atgtcctgct
    18781 gctgcggttg ccagtttgtt ccagtagtcg ttgaattcgg agaactgtcc gtcaatggta
    18841 acaccaggat tttcttcgct gtacatgtcc aggatcttct gggtacgttc atttctggtc
    18901 tgatttcccc accatgccat aaccaggctg gaggaatcac tgctgccgcc atctgactgt
    18961 gcagactggg aagtgtttgt atcagctgcg ctggtgtcag agctgcctga ggaagaagat
    19021 ccgccgcagg cggttaaaga tgcagcggca agagctgcag taagaaggac tgctaatttc
    19081 tttttcataa tattgtaacc tccttgaaat tggatccttt cgtggtccga aaggatatct
    19141 gcttgtttat gttccacatt atagcgcata atttttttgt tcttaatagt taaaacggag
    19201 cagttcgttc aaaaaaccga cttctttttt gtgcagaatt gaattatttt tgaaaaaaag
    19261 tataatgtga atgtcaggag atatacaata attaaggaag aaacgggaaa tatatgaaaa
    19321 aaaatatacg catattaatt ggccggttca ggcactggtc tttaaaatat aagatcctta
    19381 ccatagtgct ttttaacagt gtgaccatgc tgttatgtgc tgtgtttggg taccggctgt
    19441 atacaaatgc ctataataag cttctttttg actctatggc atccagactt tccgtgacat
    19501 cagctcagat ttcccagaaa atggaaaatg cagaatatat ttctaatctg atccttggtt
    19561 cttcttctat tcaggattgt ctgtcagatc ttccgtcacc ggaagacgcg ccggcgatca
    19621 cagaatataa ccgtaccatt aataatgccc ttacagaata cgcaactctt ttccggtcca
    19681 atggcattgc ttatgcagct gtttacagca aaaacgggga atatgtgaac tgtaccaact
    19741 gggtcacgtt aaagcggaca cccggttttt tgttagatgc agcaatacag aacggttatg
    19801 agaaagaggg agcagttacc tggacccatg ctaccaggca gccttatatg cttctcagca
    19861 ggaatgtacg tcagatccgg aacatgtctt tggagccttt gggaaatatg gtgatcggcc
    19921 tggacctgga acaggtagta tctgaggcag tccgttttgc ttcccagtat aaggatcaga
    19981 aagtggtgat cgccaatcag agcggaagcc tgatctatgc ttcctcttct ttaacggatc
    20041 agagggcgga ggagctgatt acagatcctt ttactaccta taagatcgtt tcttatgatg
    20101 gtcataaata ttttgccgtg aaaggaacgc ttccccatta tcagtacagt tatgtgacac
    20161 tggcgccctt tgatgaagtt gcaggcgcgc ttaccgtttc catgcagact acatttatga
    20221 tctttctggt tgggattttg tttatctggt atctttccag tcgtctgatc cgggggatca
    20281 tccgccagtt tgatgccctg atccagaaaa tgaaactgtt tacccagaat gagctggaac
    20341 tggaaccgga ggaagaggat tacggatcac agcctaatga gatcggcgct ttgcaccatc
    20401 agtttcatca gatggcatta aggatccaaa atctggtaaa ggtcaattat gtaaatgaga
    20461 tcctggcaaa ggatgcgcag ttaaaggcgc tgcgttccca gatcagcccc cattttctgt
    20521 ataatacact ggaaacgatt aactggagag cgaaagccat gggaaatgaa gtgatctccc
    20581 agatggtaga gtctctggga aatcttttaa gagtttccct ttccagccag aagaccctgg
    20641 taacactggc ctatgaactg gaactggtag actcttatat caccatccag aagatccggt
    20701 ttgaggaacg gctggagttt aaggtgatca cagatgagaa agcaaaagag ggaatgatcc
    20761 cgcctcttac cattcagcct ctggtagaaa atgccattca ctatggcatg gaagaaatga
    20821 cggaggtgtg tcatatttat ttgcaggcag aggtaaaaga gggagttctt acgatccagg
    20881 tgagaaatga aggttccaat tttgaagagg aattgctgga aaaattaaaa aataaaagca
    20941 gaagccctca tgggtttgga attggtctgt taaatatcga ccagaggatc cggcttttat
    21001 ttggggaaga gtatggactt acattatcaa atgaacatga ttttgcagta gcaacaatca
    21061 aaatgccgtt tcggaaggaa gaagagacat gctgaaatta ctgattgcag atgatgaacg
    21121 tattatacga gagacgatct ttaatattat tgactggaaa aaacatgaca ttgaagtgat
    21181 cggactttgt aaaaatggga tcgaagccta ggatatgatc ctggatgagt caccggatat
    21241 cgtacttacg gatatccgta tgccaggtat gggaggcttg gaactgatac gcaaaatccg
    21301 tcagactgac ctgcagatcc agtttattat tttatccggt tatggggagt ttgagtatgc
    21361 caaggaagcc atgagctatg gtatccgcca ttatctttta aaaccatgta atgaaacaca
    21421 gatcctggag tgtattgaac agtgccggaa agatcatgaa aaacaggcca gagaacggaa
    21481 aatgctccag cagcagttcg ttctctatga tggaatgttc cacagtgtac tttccagtat
    21541 catcaatgac tgtctggatg aaaaaatgac gctggaggag atcatttctc cctatgaaca
    21601 gtatctggat tttcattttg cagcctaccg tctgttttat gtgtattatc tggaattaca
    21661 gggtctgccg tcttttttac agcagttgaa agcatatatg gaagaaaaaa tgccccagac
    21721 cattctgtat ggaatgtatg taaaaaatac cctggtgctg tttatgcaga atgcaggtaa
    21781 agcctgtgac gatcttcatg tgtttttgca gcagctttca ccggaggggc agaagatcac
    21841 aacagagatc cgggaggaga attattcttc tttaaagaca cttcttaccc aggtaacagg
    21901 aaaacttctg cgttacagca tgatctatta cataaaccat tttcatatta cagctatctg
    21961 taattataac tgggtcattg atcagatccg tgttctgtat cagcagatcc aggagggcaa
    22021 ccgggaaaag acggatcagg cggtggaact gttaaacagt attgataata tagacttttt
    22081 aaagcagctg gcaggaaatc tatttttgaa aatggcttcg gattttcaga cgttttccac
    22141 tgtagatctg gcccagtggc tgactaagat ggaaaaagaa gaagatctgg atcctttaag
    22201 aacgatgatc tctgacaaat tccgggacct ggtaagttta aaagaaggag aagagacagt
    22261 ttcacctatg gtatcccaga tctatgtcta tgtggagcag caccttcagg attcggccct
    22321 tacgctaaaa tggatcgctg aaaattattt atttatgaat gtagattatg taagcaagaa
    22381 attttacaaa gaaaccggac agaaattttc ccagtatctt acaaaaatgc gtattggaaa
    22441 ggccaaggaa ctgatcgcaa aaaatccgga ggaaccggta aaaaatgtag cggagcaggt
    22501 aggctgtgga aacaatcccc agtatttttc acagctgttt aaaaagcaga cgggaatgac
    22561 gcctacggat tatattactt ctttgggaca ctaagcagtt tataaatgga aaaaaccgta
    22621 aaattgtgaa aacacggaaa tgtggcagaa cgacacggtt tttcggattg actattttgg
    22681 ataaaaatgg aaaataaaat catatgattt atgatcttat tttaaggagg caaaggcaaa
    22741 atgcgaatcg tctatcgggg caaggacggc agaacgggcc gggaccggat ttcgagtgca
    22801 aaaggtcggt tgtctgcc
//
LOCUS       DBMDUA010000021        22440 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1495_length_22440_cov_3.442097, whole genome shotgun sequence.
ACCESSION   DBMDUA010000021 DBMDUA010000000
VERSION     DBMDUA010000021.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 22440)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22440)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22440
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1495_length_22440_cov_3.442097"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>339
                     /locus_tag="ACZPCL_03090"
     CDS             <1..>339
                     /locus_tag="ACZPCL_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1161505.1"
                     /translation="QSGIGLNKMEDEFGYPGTARIMLKDVTLYEAKQYKDKMEDVDGV
                     DQILWCDSTVNIYSGEDFIHQEDIEDYYKDGYAVMDVTFKEGNTAKSTSQAIDELKAI
                     TGDKGCFTGMA"
     assembly_gap    340..349
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <350..2112
                     /locus_tag="ACZPCL_03095"
     CDS             <350..2112
                     /locus_tag="ACZPCL_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1161506.1"
                     /translation="KVAAAVSVVEAVERQGTIREHRQIQQVVNLQLILTVAVIMIFTI
                     LCITTTAWSEPFLFLLVMGVAILLNRGTNIFIGRVSFLTNNVAMVLQLATSMDYSIFL
                     LDAFTREKKKGLSDEEAMVDAVDAAINSIFASSLTTIAGFVALMFMKFSIGFDMGLVL
                     AKGIVFSLLTVVLFMPAMIFRFAKWNEKTAHRPFLPDFHKMGRGIYKIRNIALVIMIL
                     VVPFAYTAQGMNSFLFGNSAVGVSEGTQVYADEQTINEKFGRSNMLVAIYPNTSAITE
                     KAMSDEIEDLEYVKSVTSMANTLPEGVPEDFLPYSITSQLHTDTTSRMLIYIRTKSES
                     DKAFEYTNDIRDIVKKYYPEDSYVAGETPSTQDIKTTITADNARVNVLSLISVFVVVM
                     FSFQSVLVPIIVMIPIEAAIYINMAVPYLVGETLVYMGYIIVSSIQLGATVDYSILLT
                     NNYMACRKTMKKKEAVVEALAMSCSSVFTSGTILILAGYIVYMISSTAAIGGLGHLIG
                     RGALFSVCLVLTILPALLVLCDGIITSNEMDRFKKYLKRRHEKRKALVKSGIGAVKKK
                     ASAALARRGSQTAEVDGNEI"
     gene            2096..4366
                     /locus_tag="ACZPCL_03100"
     CDS             2096..4366
                     /locus_tag="ACZPCL_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR1161507.1"
                     /translation="MEMRFRKRVTASVLTAALLCTSAGSVPVYGAPAGADIDETMYVN
                     LDYYGKTTKVNVVKGVNLNGLGEITDYGNYINVENMSTSDAPVLGDGSVTWNLPEGQK
                     GRFYYKCTMDNEQVVLPWDFDVSYKLNGVPTDGDKLAGANGLIEINVKATPNDNADLY
                     YRNNMMLMVTVPVDMSKCYSVDADGAQIQSLGSTTAAVFSALPGEEGDYTVRIGTDSF
                     ETTGVIMAMAPGTIDDLNHIKDLKEAKDTWKDAGDALYDSLEQMAKSVESMRDGINQV
                     QSGVSSAESARQKWSANKDSILAGNDQTLESLTALSQQLETLVPHLQTAKDAAETVHN
                     SMGDIVNTMGDMQEPLRKMYNRLRNISTTSQSLGEQLNDVREDMAWLIQNNAQFQVQT
                     TTILEALPGLIEDLQDYDAEALDLYDEALNDTRSTVDDQDAGTKTGSSADAAKEDKSS
                     EAVSDATDNVSDTGENTAEANSSSDTADVAGGSDATDTTDDTDSADAADTSDTQTLSR
                     AHIEKHDVPLVAASSSVDLVTLYQMLTKIDKDSREFTQVASNLMDNVGDAAEYGADLT
                     DSMDMMIEDLTALHDSLDMYYPDLQASLDDLSELVDRTTDAMNKGVSTMTIVQNTLKA
                     TSGDMDEAAKNSLRGSMELLDKSLSILDSTTGVRTAGRTMKDVMDEQLDKFDTDNRFL
                     FIDPSEDKVSFTSDKNPAPKTLQVVLRTDEISLDDENHKETDAETEKANEGPLKRMWN
                     VLVQMWQAIISIFKNR"
     gene            4523..5089
                     /locus_tag="ACZPCL_03105"
     CDS             4523..5089
                     /locus_tag="ACZPCL_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HIR1161508.1"
                     /translation="MEPAGKQEKTKYKLAASMKECMKQMPVDKITVKNIVEGCGVARQ
                     TFYRNFLDKYDLINWYFDKLVLQSFEQIGMGHTVGESLTQKFEFIVNEKVFFTEAFRS
                     DDRNSLKEHDFELILQFYQDLIGRKTSRPLGEDLQFLLEMYCRGSVYMTVKWVLTGMK
                     DSPAKMSKKLVEAMPPRLAAVFDELKLL"
     gene            5367..6515
                     /gene="fucO"
                     /locus_tag="ACZPCL_03110"
     CDS             5367..6515
                     /gene="fucO"
                     /locus_tag="ACZPCL_03110"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HIR1161509.1"
                     /translation="MANRIVLNETSYHGAGAIAEIANEAKTRAFKKAFVCSDPDLIKF
                     GVTKKVTDVLDNAGLAYEIYSNIKPNPTIENVQTGVAAFKKSGADYLIAIGGGSSMDT
                     AKAIGIIIANPEFEDVRSLEGVAPTKKPSIPIIAVPTTAGTAAEVTINYVITDVEKKR
                     KFVCVDTHDIPVIAVVDPDMMSSMPKGLTASTGMDALTHAIEGYITKGAWEMTDMFHL
                     KAIELISKNLRGACENTKEGREGMALGQYIAGMGFSNVGLGIVHSMAHALGAVYDTPH
                     GVANAILLPTVMEYNAPCTGTKYKDIAIAMGVEGVENMSQEEYRKAAVDAVKKLSADV
                     GIPADLKAIVKAEDVDFLAQSAMDDACRPGNPKDPTFEDIKNLYLSLM"
     gene            6923..8590
                     /locus_tag="ACZPCL_03115"
     CDS             6923..8590
                     /locus_tag="ACZPCL_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR1161510.1"
                     /translation="MGVELLLGLLGGLALFLYGMQMMSNNLEAAAGNKMKQILEKLTT
                     NRFMGILVGAGITALIQSSSATTVMTVGFVNSGLMSLNQAVWIIMGANIGTTITGQLI
                     ALDVGALAPLIAFIGVVLVVFVKNKKLQHIGGIVAGVGVIFIGMGMMSDAMLPLRDEP
                     EFIQLMTSFKNPLLGVMAGALFTAVIQSSAASIGILQALAVSGVIGLHSAVFVLFGQN
                     IGTCITAVLASVGTNRNAKRTTAIHLMFNVVGTVIFVVLCMVTPFVDWMITTNPANPA
                     AQIANVHTIFNIATTVILFPFGPMLVNIAKKILPDTQVKHVMDADQWFEGLMASKHHL
                     GVSTIAISQIHEEIRDMLAIAASNVSDSFKAMEQGPGENGIQEIADREEEIDLSNVRL
                     SKKISKVLVLDQTPQDIEALNKMYSILGNIERIGDHAMNLAEYAQTIQEKNLNFSDYA
                     KNEFKVMDESCALGMELLRKAAAGDSDFTLEKIQAIEQKIDDITDNFRQNQIDRMREG
                     HCNVESSILYSEMLTDYERIGDHMLNIGEAYDAIQWNSDKAAALAEE"
     gene            8815..9696
                     /gene="pdxS"
                     /locus_tag="ACZPCL_03120"
     CDS             8815..9696
                     /gene="pdxS"
                     /locus_tag="ACZPCL_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005345616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HIR1161511.1"
                     /translation="MNTNTERYELNKQLAQMLKGGVIMDVTTPEQAKIAEEAGACAVM
                     ALERIPADIRAAGGVSRMSDPKMIKGIQEAVSIPVMAKCRIGHFVEAQILEAIEIDYI
                     DESEVLSPADDVFHIDKRKFKVPFVCGAKDLGEALRRINEGASMIRTKGEPGTGDVVQ
                     AVRHMRMMNQEIAKIVSMREDELFEEAKVLRVPYDLVEYVHKNGRLPVVNFAAGGVAT
                     PADAALMMQLGAEGVFVGSGIFKSGDPKKRAAAIVKAVTNYTDQKMLAELSEDLGEAM
                     VGINEQEIKLLMAERGK"
     gene            9698..10270
                     /gene="pdxT"
                     /locus_tag="ACZPCL_03125"
     CDS             9698..10270
                     /gene="pdxT"
                     /locus_tag="ACZPCL_03125"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HIR1161512.1"
                     /translation="MKIAVLAVQGAFIEHEKKLEKLGAQCIELRKKENLEEDFDGLVL
                     PGGESTVQGKLLKELGMFDALKEKIEAGMPVLATCAGLILLAKDLTNDDHRYFATLPV
                     SVRRNAYGRQLGSFHTTEEFEGIGKVPMTFIRAPFVEKAEEGVKVLAKADGNIVAVQY
                     KNQIGMSFHPELDEDDRIHQLFLDTVKKNK"
     gene            10299..10424
                     /locus_tag="ACZPCL_03130"
     CDS             10299..10424
                     /locus_tag="ACZPCL_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161513.1"
                     /translation="MPKTKWNLNTIYISERLQESLRPISRSASSQMSRPAISSST"
     gene            10606..10845
                     /locus_tag="ACZPCL_03135"
     CDS             10606..10845
                     /locus_tag="ACZPCL_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HIR1161514.1"
                     /translation="MKRRYLKDVTTLPDYILQVDFVSGSRLLLDMKPYLDKIRFLSLA
                     DKNVWSSVVTNGIFVRFGDVELSHDEILSMAEQEH"
     gene            10876..11625
                     /locus_tag="ACZPCL_03140"
     CDS             10876..11625
                     /locus_tag="ACZPCL_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161515.1"
                     /translation="MKKWKSKLFPVITLAVCLGLAACGGGAESEGSSDDQEDYEETEN
                     SAPESFEGMVKDPDITAVYDMYWGTWIGEDDSELIVGLNGSGEDRFEWYDEDDNLMAS
                     GYIQLVPEYEADYFYNEHDGWAHHVWRDEDGALHIDSFGVFTKKPLGRDVSESDCEEL
                     AGVWFLDGEADAASVIEIEKDGSWSLSERPDGDGDPAEVDSGILQVNERGDDQYFAVS
                     EQFDDVFYPITLAEPGAMYWGYTNEYYERSE"
     gene            11600..11950
                     /locus_tag="ACZPCL_03145"
     CDS             11600..11950
                     /locus_tag="ACZPCL_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161516.1"
                     /translation="MNTMKEVNDQQKEMALKKKIYLLPVPALLLCLYLAACSSLVDTR
                     SFGSALPDGLEPVTDMAFLDGTWSIAGTTKLYFDSSGGYYIYRGCNGLGGRGEFSEVD
                     GRPMIEFNGRRHPY"
     gene            12164..12607
                     /locus_tag="ACZPCL_03150"
     CDS             12164..12607
                     /locus_tag="ACZPCL_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161517.1"
                     /translation="MGGALPFTNMQNLRTENNEDGTYRYEDFTQDGQILVVNTVEPTR
                     FVPEVQDLEDYLTACAFALSDTDTYELLSAEENEEYTENLTYPVYIVTYTAGENEDTR
                     KWTVFAMDTDRYTYLYGFCEAVDTEEDMDEIYQSIFSQLYLSDEN"
     gene            12621..13496
                     /locus_tag="ACZPCL_03155"
     CDS             12621..13496
                     /locus_tag="ACZPCL_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161518.1"
                     /translation="MKKKMPYVLAGILLMISLVCMACGSTQEATALPDDANESIQDEE
                     DEDLQNDPNDGLGGDLNEDTVNDFSGFEGIWLGEANNDYDYMEFDSEGNWTLYLSGEV
                     MDEGYLRYEPEWEAIYAYSNRDDSGSRIAMEEGQLYSAAYGYFNPGKGMEYLWYKDGG
                     EFTEDDEPVAYVENGSGGSGNQNAHPDSYWSWDSDLCQRNVSEFEGVWYYDGDLSAEM
                     YVVIDGNGDWSYYQRAPGAEAAEMDHGIFTYSTDETSTYYADSTVYDGVSYKVFDFDS
                     DMLIWGDEGAYYRME"
     gene            13516..14655
                     /locus_tag="ACZPCL_03160"
     CDS             13516..14655
                     /locus_tag="ACZPCL_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161519.1"
                     /translation="MKKRQIKRFFLTVPVVLGISLVACGASGDVAGDDWRTSGVVAGS
                     GTITHDGESDVLVSFIDEYGDNLSTITIGEEDQDTAEETDPAMNLEDYVGLWEYQGEN
                     RWLRIHDDSTWEFVNDQDDVIESGTLWVEENGITLHFDGSGDTLQLDLTISGDLMDLT
                     NGGSLFPVEAIQSSVPYFTRNGLEMNAEVELGTFLLANGASSYSGEGNGYRRSDCYWE
                     VTKKADDIHNGIRDLHFDAICYIPESAISTAANGTVVVSSELYDAYTGMWLTAGSTFN
                     NSSRGDNYYLHTVSWRGNSYLIEFTYSTDWNFQVGDWGAVLTKSYSVYMPADYDGLVF
                     AAQAEPDTYQESARKMQLDSISPEACVLDLDTLEPYSSLYFSLCD"
     gene            14667..15647
                     /locus_tag="ACZPCL_03165"
     CDS             14667..15647
                     /locus_tag="ACZPCL_03165"
                     /inference="COORDINATES: protein motif:HMM:NF022779.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HIR1161520.1"
                     /translation="MKKKNRASVSSKPIKMTDAELSKKLGHYQAMSVIWFWVGVVGAV
                     SGAIFYFVLQNKALKSLLVAVLFFGGIFCASFFSGGAQKKLKVLMQEQMGDFFRAEWE
                     KAFGPDIHTQEMRIDEPFMRAHHLLDGKWEECTVENFHEGDHGGIHFSAANVQLDHVY
                     QRGCSHDGYETCRKMVFKGIVLRCRTWVPVPSPVLANVRTEDSPCGVLTGNNIFDCSF
                     CVTANSKQDAACRLTPRFIDFLTKFNRDVEGQILSFCWEGKIFSLVLETDFGIATIAS
                     SVDLSDLDAARRSYIRSLKELGSVLDRLIEGPALTDVVEREEYGRTENDR"
     gene            15622..16008
                     /locus_tag="ACZPCL_03170"
     CDS             15622..16008
                     /locus_tag="ACZPCL_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161521.1"
                     /translation="MAEQKMTDEQLSQELHKRNGGRAGGTLLSLFGGTMLFSLCSQSV
                     AAAAGTQTGSSITVGTGNLCEHHPEHDDECGCSEGTTGTACSHEHTDDCYTEVLKCVH
                     KHDDECGYSPATRGTPCEFAWDGKEK"
     gene            16041..16565
                     /locus_tag="ACZPCL_03175"
     CDS             16041..16565
                     /locus_tag="ACZPCL_03175"
                     /inference="COORDINATES: protein motif:HMM:NF023580.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HIR1161522.1"
                     /translation="MKRVKILVCGFSLAIALVALPFPYIDDITSGLGFSIAAVLFAWL
                     GILERRGAKNGYKRDVRLYSASTMMKVVHVEESEYDRWEDQPDGSSKLRREKVYLPTY
                     EYSVNGKTYRYLSFQSVSSKRDVGKQVVGYYKPDRPDLITENRPRKPVFGGFLYFYGA
                     AICLFFGIVTFTGM"
     gene            17143..17379
                     /locus_tag="ACZPCL_03180"
     CDS             17143..17379
                     /locus_tag="ACZPCL_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR1161523.1"
                     /translation="MITATATAMQNNFGRYLNLVMSGQEIIVTKNGKEVGRFIPKEAA
                     VSYLTDSLTGILKEKNDLAKIKEEDLKAKYGIVD"
     gene            17405..17770
                     /locus_tag="ACZPCL_03185"
     CDS             17405..17770
                     /locus_tag="ACZPCL_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR1161524.1"
                     /translation="MNRQEFVTDSSMIWKLCETEQAKGYISVLTYANIMYIMRKQLTP
                     EKIEEVFHKLNLIFEFTDFSPMILEKSVNMKWPDFEDAVQSATAEAIHADYIVTRNIK
                     DFVKSKVVAFTPGELLARL"
     gene            complement(17901..18557)
                     /locus_tag="ACZPCL_03190"
     CDS             complement(17901..18557)
                     /locus_tag="ACZPCL_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HIR1161525.1"
                     /translation="MGNSISIFFLIEAVGTIAFASSGAMVAIRQNLDLLGIIVLGVTT
                     AVGGGMMRDILLGIVPPSLFTNPIYTIMAFITVMVLFIVIRMNQHFLEGDCIVTYEKL
                     MNIFDAIGLAAFTVTGIDTAVMAGYGDYHFLSVFLGVLTGVGGGLLRDIMARQTPYIL
                     KKHVYACASIAGGICYSFLLSSPVNNDIAMITSAALVIAIRLLATHYCWNLPRALKQS
                     "
     gene            complement(18579..19586)
                     /gene="asnA"
                     /locus_tag="ACZPCL_03195"
     CDS             complement(18579..19586)
                     /gene="asnA"
                     /locus_tag="ACZPCL_03195"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HIR1161526.1"
                     /translation="MELIIPEHYDPRLDIRETQDAIKYIRDTFQKEMGREMHLERISA
                     PLFVEKSSGLNDNLNGVERPVSFDMAAIPGETMEVVHSLAKWKRMALKEYGFQPGEGL
                     YTNMNAIRRDEELDNLHSCYVDQWDWEKVINKEDRNEKTLKDTVRLIFKIIKHMQHEV
                     WYKYPQAVNQLPDDIFFITTSELEAMYPDKTPKERENLITKEHGCVFLMQIGDKLASG
                     KPHDGRAPDYDDWKLNGDILFWYPTLNCALEISSMGIRVDEVSLREQLEKSGCMDRME
                     LPYHKMLLNGELPYTIGGGIGQSRLCMLLLDRAHIGEVQASIWPKEMRDTCKKHKIYL
                     L"
     gene            19844..21037
                     /locus_tag="ACZPCL_03200"
     CDS             19844..21037
                     /locus_tag="ACZPCL_03200"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR1161527.1"
                     /translation="MKKFKRIFVIVMDSFGAGHAKDGAAYGDDGADTMGHIAEKHDNW
                     KIPNLTRLGLANLHPLKGVTPAEHPLGYQLVLNEKSCGKDTMTGHWEMMGIYTTKPFV
                     TFTDHGFPPELIHELEERTGHKIIGNKSASGTEILDELAEEEIATGHMIVYTSADSVL
                     QICGNEETFGLDELYRCCEIARELTLKDEWRVGRVIARPYVGKKKGEFKRTSNRHDYA
                     LKPTGKTALDALKEKGYDVISVGKIRDIFDGEGITEAYKSKSSVHGMEQTIDIAKNED
                     FTGLCFINLVDFDALWGHRRNPEGYAKEVENFDVKLGELLQDLKEDDLLMITADHGND
                     PTWTGTDHTREQVPLLVYSPSLKEGGKLPETDTFGVIGATVADNFEVKMPEGTIGTSI
                     LEQFK"
     gene            21204..21866
                     /locus_tag="ACZPCL_03205"
     CDS             21204..21866
                     /locus_tag="ACZPCL_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR1161528.1"
                     /translation="MENTKAEVREKIKEAQKVLIGIGKEWALRDDEKDIRFRHLSDPA
                     QSDLKAAYEELYQLIQDKDYYIVTTLTDGAIYEMPFDKNRIVAPCGNIHWRQCSKACT
                     KDIWEEGEVPDDICPHCKAPLTGNTLKAETYIEEGYLPRWKDYMKWQTGTINRSLVIL
                     ELGEGFSTPTVMRWPFEKIIYFNQKSKLYRINENFYQLPKEAEERGVSVHENSVRWML
                     EK"
     gene            21927..>22440
                     /gene="hisH"
                     /locus_tag="ACZPCL_03210"
     CDS             21927..>22440
                     /gene="hisH"
                     /locus_tag="ACZPCL_03210"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR1161529.1"
                     /translation="MIAVIDYDAGNLKSVEKALIFLGETPVITRDREELLAADKVILP
                     GVGAFGDAMDRLHQYGLVDVIKEIVKKGIPFLGICLGLQLMFESSEESPGVEGLGLLP
                     GKILKIPETPGMKVPHMGWNSLKIDPNSRLFKGIPDGSYVYFVHSYYLKAGSEDIVAA
                     TTEYGTHIHAA"
BASE COUNT         6661 a         4363 c         5726 g         5680 t
ORIGIN      
        1 cagtcaggta tcggtttaaa taagatggaa gatgagtttg gctatcccgg aacagcccgt
       61 attatgctaa aggatgtgac gctatacgaa gccaagcaat acaaagataa aatggaagat
      121 gtagacggtg tggatcagat cctctggtgt gacagcaccg taaatatcta ctcaggtgaa
      181 gattttatcc atcaggaaga cattgaagat tattacaaag atggctacgc agtcatggat
      241 gtaactttta aagaggggaa tacagcaaaa agcacttccc aagccattga tgagttaaaa
      301 gccatcacag gagataaagg ctgctttaca ggaatggcan nnnnnnnnnt taaagttgca
      361 gctgccgtca gcgtggtaga ggctgtcgag cggcagggca cgataagaga acatcggcaa
      421 atccagcagg tcgtcaattt gcagctgatc ttaacagtag cagttatcat gatctttacc
      481 atcttatgta tcacaaccac tgcctggtca gagccgtttc tgttcttact ggtcatgggt
      541 gtggccattc tgttaaaccg tggtaccaac atttttatag gaagagtttc cttcctgacc
      601 aacaacgtgg caatggtgct ccagttggca acttccatgg actactccat tttcctgtta
      661 gatgcattta ccagagaaaa gaaaaaaggg ctttctgatg aagaagccat ggtagatgcg
      721 gtagatgcgg ccattaattc tattttcgcc agcagcctta ctactatagc aggtttcgtg
      781 gccctgatgt tcatgaaatt cagtattggt tttgatatgg gtctggtact ggcaaaagga
      841 attgtattca gtcttttaac agttgtactt ttcatgccgg ccatgatctt ccgctttgcg
      901 aaatggaatg aaaagacggc acatcgtccg ttcctgccgg atttccataa aatgggaaga
      961 ggcatttaca agatccgtaa tattgcactg gtgatcatga tcctggtagt tccttttgct
     1021 tatactgccc agggaatgaa cagtttctta ttcggaaaca gtgcagtagg tgtcagcgaa
     1081 ggaacccagg tctatgcaga tgagcagacc attaatgaga aatttggccg cagcaatatg
     1141 ctggttgcca tttatccaaa tacatcagct attacagaaa aggcaatgtc tgatgagatc
     1201 gaagatctgg aatatgtaaa aagcgtaaca tccatggcaa atacactgcc ggaaggtgta
     1261 ccggaagatt tccttcccta ttccattacc agccagctgc atacagatac cacaagccgt
     1321 atgctgatct acataaggac caagtcagaa agtgacaagg cttttgagta taccaatgat
     1381 atccgtgata ttgtgaagaa atattacccg gaagactctt atgtggcagg tgaaactcct
     1441 tctacgcaag atattaagac tacgatcaca gcggataatg caagagttaa tgtgctgtcc
     1501 ctgatcagtg tatttgtggt agtcatgttc agtttccagt ctgtactggt gcccatcatt
     1561 gttatgatcc ctattgaggc agctatttat atcaatatgg cggtgcctta tctggttggt
     1621 gagacgctgg tttatatggg ctatattatc gtaagcagta tccagctggg ggctacagta
     1681 gactattcga tcctgcttac taataactat atggcctgca gaaagaccat gaagaagaag
     1741 gaagctgtgg tggaggcact ggccatgtcc tgttcctcag tatttacttc gggaactatc
     1801 ctgatactgg caggttacat tgtatatatg atctccagta cggcggctat aggcggactg
     1861 ggacatctga tcggaagagg tgcattgttt agcgtgtgtc tggtacttac cattttgccg
     1921 gcactgctag tcctctgtga cggtataatc acaagtaatg aaatggaccg ctttaagaag
     1981 tatttaaaac gaaggcatga aaagaggaaa gccttagtaa aatccggaat tggggcagtt
     2041 aagaaaaaag cgtcagcagc cctggcaaga agaggcagtc agacagcgga ggtagatgga
     2101 aatgagattt agaaaaagag tgacagcttc tgttttaacg gcagcattgc tttgcacatc
     2161 agcaggcagc gttcctgtat atggcgcacc ggcaggtgca gatatagatg agaccatgta
     2221 tgtgaacctg gattactacg gaaaaacaac aaaggtaaac gtagtaaaag gggtaaattt
     2281 aaatggcctt ggtgagatca ctgattatgg aaattacatc aatgtagaaa atatgagtac
     2341 cagtgatgct ccggttttag gagatggaag tgtaacctgg aacctgccgg aaggtcagaa
     2401 gggccgtttc tattataagt gtaccatgga caatgaacag gtggtacttc catgggattt
     2461 tgatgtgtcc tataagttaa acggtgttcc aacagatggt gacaaactgg caggtgcaaa
     2521 cggcctgatc gagatcaatg tgaaggctac gccaaatgat aatgctgatc tgtattaccg
     2581 taacaacatg atgttaatgg tgacagttcc tgtagatatg tctaaatgct acagtgtaga
     2641 cgcagatggc gcccagatcc agagccttgg atccacaaca gcagctgtat tttccgccct
     2701 tccgggggaa gaaggagatt atactgtccg tattggtaca gacagttttg aaactaccgg
     2761 tgtgatcatg gcaatggcac ctggcactat tgatgacctg aaccatatta aggacctgaa
     2821 agaagcaaag gatacatgga aagacgcagg agatgcttta tatgacagtc tggagcagat
     2881 ggctaaatca gtggagtcca tgcgtgacgg catcaaccag gtacagtccg gtgtttcttc
     2941 tgctgaaagc gcaagacaga aatggagtgc caataaagac agtattttag ctggaaatga
     3001 tcagactctg gagtcactga cagcgctgtc acagcagctg gaaactctgg tcccacattt
     3061 gcagactgct aaggatgcgg cagagaccgt gcataacagc atgggtgata ttgtaaatac
     3121 catgggagat atgcaggaac ctctgcgcaa gatgtataac cgtctgcgca atatcagcac
     3181 cacttcccag tctttagggg aacagttaaa tgatgtaaga gaagatatgg catggctgat
     3241 ccagaacaat gcccagttcc aggtacagac cactactatt ttggaggcgc ttccgggact
     3301 gatcgaagac cttcaggact atgatgcaga agcactggat ctgtatgacg aagctctgaa
     3361 tgatacaaga agtacagtgg atgatcagga tgccggtaca aagacaggca gcagcgcaga
     3421 tgcagcaaaa gaagataaga gcagcgaagc tgtttctgat gccacagata atgtttctga
     3481 tacaggggaa aatacagcag aggcaaatag cagttcggat acagcagatg ttgcaggtgg
     3541 ttcagatgcc acagacacaa cagatgatac agacagcgca gatgcagcag ataccagtga
     3601 tacacagaca ctcagccggg cgcatattga aaaacatgat gtgcctctgg tagcagcttc
     3661 atccagtgtg gatcttgtga ccctttatca gatgttaaca aagatcgata aggacagccg
     3721 tgaatttact caggttgcat ccaatctgat ggataatgta ggagatgcag cagaatatgg
     3781 tgcagatctg acagacagca tggacatgat gatcgaagat ctgacagctc ttcatgactc
     3841 cctggatatg tattatccgg atctgcaggc atccttagat gacctttctg agctggtaga
     3901 ccgcaccaca gatgccatga acaaaggtgt cagcactatg accattgtgc agaacacatt
     3961 aaaggcaacc agcggtgata tggatgaggc agcaaagaac agtttaagag gcagtatgga
     4021 actgcttgat aagagcctca gcatcttaga cagtactaca ggtgtgagaa cagcaggccg
     4081 taccatgaaa gatgttatgg atgagcagct ggataaattc gacaccgata accgcttcct
     4141 gttcattgat ccgtcagaag ataaggtttc ctttacttct gataagaacc cggcccctaa
     4201 gaccttacag gtagtccttc gtacagatga gatcagtctg gatgatgaaa accataagga
     4261 aaccgatgca gaaacagaga aggcaaatga aggcccgtta aagagaatgt ggaatgttct
     4321 ggtacagatg tggcaggcca tcatctctat tttcaagaac agatagttta aaaacagata
     4381 gtcacagccc catgtttctg gtatgataga aataagggtt ggaaaaaata cagaccctcg
     4441 gtatgtgaaa tggcatgccg ggggccttgt gctgaaattc tgtccgtggg cagattacat
     4501 agctgtatag tgggaggaaa acatggaacc ggcaggaaag caggaaaaaa cgaaatacaa
     4561 actggcagca tccatgaaag aatgcatgaa gcagatgccg gtggataaga taactgtaaa
     4621 aaatattgtg gaaggctgcg gcgttgcaag acagaccttt taccggaatt ttttagataa
     4681 atatgacctg atcaactggt attttgacaa actggtgctc cagtcctttg agcagatcgg
     4741 catgggacat acagtaggag aaagcctgac acagaaattt gaatttatcg tcaatgaaaa
     4801 agtgttcttt acagaggcat ttcgcagtga cgaccgcaac tccttaaaag aacatgattt
     4861 tgaactgatc ttacagtttt atcaggatct gatcggacga aagaccagca gacccttggg
     4921 agaagatctg cagttccttt tggaaatgta ttgcaggggt tctgtgtata tgacagtaaa
     4981 atgggtcctt acaggaatga aagactcacc ggcgaaaatg tcaaagaagc tggtggaagc
     5041 tatgcctcca agactggctg ctgtatttga tgagctgaaa ctgctgtaaa aacggaaaac
     5101 tttgttatat ataacacaaa acgataatat cgatatacgg ataatataag aatgtatacg
     5161 tgatcattta tgtgtgattt ttataataaa acttatataa aacaaaaaat attctcaaaa
     5221 agtgacagac aagtaagttt gtcactttta tttttgttaa cgtgttgtta taatattaac
     5281 gaaacaaagg aaaacaacat gaaatacaaa actgctcagg atcttgtatc cagagagtat
     5341 attttcataa aaaaggagaa tgtatcatgg caaacagaat cgtattaaat gaaacttctt
     5401 atcatggcgc aggcgctatc gctgagatcg ctaacgaagc aaaaacaaga gctttcaaga
     5461 aggctttcgt atgttcagat ccagatctga tcaaatttgg cgtaacaaag aaagtgactg
     5521 atgtactgga caatgcaggt cttgcttatg agatctactc caatattaaa ccaaacccaa
     5581 ccatcgaaaa cgtacagacc ggtgtagcag catttaagaa atccggtgct gattatctga
     5641 tcgcaatcgg cggtggttct tctatggata cagcaaaggc aatcggcatc atcattgcta
     5701 acccagaatt tgaagatgta agaagcctgg aaggcgtagc tccaaccaag aagccatcta
     5761 tcccgatcat cgcagttcca actacagcag gaaccgcagc agaagttact atcaactacg
     5821 ttatcactga cgtagaaaag aaacgtaaat tcgtttgtgt agatacacac gatatccctg
     5881 taatcgcagt tgttgacccg gatatgatgt cctctatgcc aaagggactg accgcatcta
     5941 ccggtatgga cgctttaaca catgcaatcg aaggatacat caccaagggc gcatgggaga
     6001 tgaccgatat gttccactta aaggcaatcg agctgatctc caagaactta agaggcgctt
     6061 gcgagaatac aaaagaaggc cgtgaaggaa tggctttagg acagtacatc gcaggtatgg
     6121 gcttctccaa cgtaggttta ggaattgttc attccatggc acatgcttta ggtgctgttt
     6181 atgatactcc tcatggtgtt gcaaatgcaa tcctgcttcc aactgttatg gaatacaacg
     6241 caccatgcac aggaaccaag tacaaagata tcgctatcgc aatgggcgtt gaaggcgtag
     6301 agaatatgag ccaggaagaa taccgcaagg cagctgtaga tgcagttaag aagctgtccg
     6361 ctgatgttgg tatcccagct gacttaaagg ctatcgttaa ggcagaagat gtagacttcc
     6421 ttgcacagtc cgcaatggat gatgcgtgcc gtccaggcaa tccaaaggat ccgacctttg
     6481 aggatattaa gaacttatat ctgtctctga tgtaagggca gaagagaaaa acaagcgacc
     6541 gtcccgtttt cggggcggtc atttgttttt cttctgccct taacgaatgg cccgcctgcg
     6601 ccagcaggca gtggagcgcg tcagcgcgtg actgtgagtg gaccatatgc accccaccgc
     6661 cctgctcggg gcggtctttt tttgcttcaa aactgttaaa atatattatc gatatatgga
     6721 taatatagcg taattttaca aacaatttac ctgaacttta cataaccgtc caacaatccg
     6781 tattgacgac tgagaaaata tcccgtataa tgagcaagtg taaactcaaa gtaaaaatca
     6841 agtaaagaac aggaaaaaac atatattatc aatatataga taatgtaaaa ataccctgtt
     6901 cattaggaaa aaggagtaaa aaatgggagt tgaattgctt ttgggccttc tgggaggtct
     6961 ggcgctgttc ctgtatggaa tgcagatgat gagcaacaat ctggaagcag ctgccggaaa
     7021 caagatgaag cagatcctgg aaaagctgac caccaaccgc tttatgggaa tcctggtagg
     7081 cgcaggcatc acagcactga tccagtcatc ttcagctaca acggttatga ccgtaggctt
     7141 tgtaaactca ggtcttatgt cacttaacca ggctgtatgg atcattatgg gtgctaatat
     7201 cggtaccacc attaccggac agctgatcgc attagacgta ggcgctttag caccgctgat
     7261 cgcatttatc ggtgttgtac tggtggtttt tgtaaagaat aaaaagttgc agcacatcgg
     7321 cggcattgtt gccggagtag gcgttatctt catcggtatg ggaatgatga gcgatgcaat
     7381 gcttcctctt cgtgatgaac cggaatttat ccagctgatg accagcttta aaaacccgct
     7441 gttaggtgtt atggcaggtg cgctgtttac tgcagtgatc cagtcttccg cagcttctat
     7501 cggcatctta caggcactgg ctgtaagcgg cgtgataggc cttcacagcg ctgtgttcgt
     7561 actgttcggt cagaatatcg gtacttgtat cactgctgtt ctggcttctg tgggaaccaa
     7621 cagaaatgca aaacgtacta cagccatcca tctgatgttc aacgtagtcg gtacagttat
     7681 ctttgtggta ctgtgtatgg taacaccatt cgtagactgg atgattacca ctaacccggc
     7741 aaatcctgct gcacagatcg caaatgtgca taccatcttt aatattgcca caactgtaat
     7801 attattccca tttgggccga tgttagtaaa tattgcaaag aagatccttc cggataccca
     7861 ggtaaagcat gtgatggatg cagaccagtg gtttgaaggc ctgatggctt ccaagcatca
     7921 tctgggtgtt tctaccattg ccatcagcca gatccatgaa gaaatccggg atatgctggc
     7981 tatagctgct tccaatgttt ctgacagctt taaggccatg gaacagggac cgggtgaaaa
     8041 tggtatccag gagatcgcag acagagaaga ggagatcgat ctttccaatg tacgtctgtc
     8101 aaagaagatt tccaaggttc ttgttttaga ccagacacct caggatattg aagcattaaa
     8161 taagatgtac agtattttag gaaatatcga gcgaattggc gatcatgcaa tgaacctggc
     8221 agaatatgcc cagaccattc aggaaaagaa cctgaacttc tctgattatg caaaaaatga
     8281 atttaaagtc atggacgaaa gctgtgcatt aggtatggag cttctgagaa aggcagcagc
     8341 cggagacagt gactttaccc tggagaagat ccaggcgatc gagcagaaga tcgatgatat
     8401 caccgacaac ttccgtcaga accagatcga ccgtatgcgc gaaggccact gtaatgtaga
     8461 gtcctctatc ctgtattctg aaatgcttac agattatgag cgtattggtg accatatgtt
     8521 aaacattggt gaagcatatg atgcaatcca gtggaacagt gataaagcgg ctgctcttgc
     8581 agaagaataa acgtatccaa taaagaaaaa taataacaaa tatcccaagc tgtatttact
     8641 gaacaaccgg tagatgcagc ttaatttata tgcaaactgg actggttaaa aatatgataa
     8701 ttggctatta taaaaagtcc acatatacgt tatactctag tcaatccaaa acgtttcggg
     8761 ccaatcaata aaatatttca aaaatatatc agataagacc aggaggtttg aaagatgaat
     8821 acaaatacag agagatacga attaaataag cagctggcac agatgttaaa gggcggcgtg
     8881 atcatggacg ttaccacacc ggagcaggca aagatcgcag aagaagcagg cgcatgtgcg
     8941 gttatggctt tagagcgtat tcctgcagat atccgcgcag caggcggcgt atcccgtatg
     9001 agtgacccga agatgatcaa gggcatccag gaggcagttt ccattcctgt tatggcaaaa
     9061 tgccgcattg gacattttgt agaggcacag attttagaag ccattgagat cgactacatt
     9121 gatgaaagtg aagtattatc ccctgcagat gatgtattcc atattgataa gagaaaattc
     9181 aaagttccgt ttgtatgcgg cgcaaaggac ttaggagaag cattaagaag gattaacgaa
     9241 ggcgcttcca tgatccgtac aaaaggcgaa cctggaaccg gtgatgttgt ccaggcagta
     9301 cgtcatatgc gcatgatgaa ccaggagatc gcaaagatcg tatccatgag agaagatgaa
     9361 ctgtttgaag aggcaaaagt attaagagtg ccttatgacc tggtagaata tgtacataaa
     9421 aacggccgtc ttccagttgt aaactttgca gcaggcggtg ttgcaacacc tgcagacgca
     9481 gctcttatga tgcagttagg agcagaaggc gtattcgtag gttccggtat ctttaaatca
     9541 ggagatccaa agaagcgtgc agcagccatt gtaaaggcag ttaccaatta tacagaccag
     9601 aagatgttag cagagctttc tgaggactta ggagaagcca tggtaggcat caatgagcag
     9661 gagatcaagc ttttaatggc agaaagagga aaataagatg aagatagccg ttttagcagt
     9721 tcagggcgct ttcattgagc atgagaaaaa gcttgaaaag ctgggagccc agtgtatcga
     9781 actgcgaaaa aaagaaaacc tggaagagga ttttgacggt ctggtacttc ccggcggtga
     9841 gagtacggtt cagggaaagc tgttaaaaga acttggcatg tttgatgctt taaaagaaaa
     9901 aatagaagcg gggatgccgg tacttgctac ctgtgcagga ctgatccttc tggcaaaaga
     9961 ccttaccaat gatgaccacc gttattttgc tacacttcct gtttccgtaa gaagaaatgc
    10021 ttatggaaga cagcttggca gctttcacac aacagaagaa tttgaaggca ttggaaaagt
    10081 gcctatgacc tttatccggg cgccttttgt ggaaaaagca gaggaaggcg taaaagtgct
    10141 tgcaaaagca gatggaaata ttgtagctgt acagtataaa aatcagattg gaatgtcctt
    10201 tcatccggaa ctggatgaag atgaccgtat ccaccagttg tttttggaca cagtaaaaaa
    10261 gaataaataa gacgatgaaa taaaaatggg gtgtgcatat gccaaaaaca aaatggaatc
    10321 tgaataccat atacatatcg gaaagactgc aggagagcct gcggcccatc tcccgcagcg
    10381 ccagcagcca gatgagccgt cctgccatca gcagcagtac ataaatcaga tttattccaa
    10441 acttcatatt gagggcgata ccagaacaaa aaggagtcag cttgtagagc tgttcttacc
    10501 aaatccctaa cctttagtta atggttatct cataggatcc accgtacaat atcagtacgg
    10561 tggatttttt aatatcttta tgacacggca gaaaggaggg tactcttgaa acggagatat
    10621 ctaaaggatg ttacaacgct gcccgattat attttgcagg tcgattttgt atcgggaagc
    10681 cggcttttgc tggacatgaa gccttatctg gataagatcc gttttctttc gcttgcggat
    10741 aagaacgtgt ggagtagtgt tgtgaccaac ggtatctttg tccgcttcgg agatgtggag
    10801 ctgagccacg atgaaatatt gtccatggca gaacaggaac actaacgcag ctttgcgaaa
    10861 agggaggaat gcaggatgaa aaaatggaaa agtaagttat ttcccgtaat cacactggca
    10921 gtgtgtctgg gccttgctgc ctgcggtgga ggggctgaaa gcgaaggcag cagcgatgac
    10981 caggaagact acgaagagac tgaaaacagt gctccggagt cttttgaagg aatggtgaag
    11041 gatccggata tcacggcagt ttatgacatg tactggggta cctggatcgg cgaagatgac
    11101 agcgaactga tcgtgggttt aaacggcagt ggggaagacc gctttgaatg gtacgacgag
    11161 gatgacaacc tgatggccag cggttatatc cagcttgtgc cggagtatga agcagactat
    11221 ttctacaatg aacatgatgg ttgggctcat catgtctggc gggatgagga tggggcactt
    11281 catatcgact ccttcggtgt gtttaccaag aaacctctgg ggagagatgt aagtgaaagt
    11341 gactgtgagg aattggccgg ggtctggttt ttagatggag aggcagatgc ggccagtgtg
    11401 atcgagatcg aaaaggatgg cagttggagc ctttcagagc gcccggatgg tgacggggat
    11461 ccggctgaag tggattcagg aatactccag gtaaatgagc ggggcgatga tcagtacttt
    11521 gccgtttctg agcagtttga cgatgtgttt tatccgatca ccttagcgga accgggagct
    11581 atgtactggg gatatacaaa tgaatactat gaaagaagtg aatgaccagc agaaagagat
    11641 ggccttaaaa aagaaaatat atttgcttcc tgttccggcc ttgctcctgt gtttgtatct
    11701 ggcagcctgc agcagcttgg ttgatacaag atccttcggc tccgctttac cggacggctt
    11761 ggagccggtc acagatatgg ctttccttga tggtacctgg agcattgccg gaaccacaaa
    11821 gctgtatttt gacagcagcg gcggctatta tatctatcgc ggatgtaatg gccttggcgg
    11881 ccgcggagag ttttcagaag tggacggcag acccatgatc gagtttaacg ggagaagaca
    11941 tccgtattag agaaacggat gggaaagact ggggagaaaa ccgtctgaaa acagcactgg
    12001 ctgagggcga aagcgctaag atcgatctgg gaaaatatac gatggcagaa ctgggcagag
    12061 gttttcaggt tcagttttac ggcgaggatg aaaagccggt caatccgaac tatgatcctg
    12121 atactgcgac gatctgatcc cgagtgaacg cattcctgtt ttgatgggag gagcgctgcc
    12181 ctttaccaac atgcaaaacc ttcgcacgga aaataatgaa gatggaacat accgctatga
    12241 ggatttcacg caggatggcc agatccttgt ggtaaatacg gtagagccga cccggttcgt
    12301 tccggaggtc caggatctgg aagattatct gacagcctgc gcttttgcct taagtgatac
    12361 ggatacctat gaactgttat ctgcagaaga aaatgaggaa tataccgaga acttaacata
    12421 cccggtgtat atcgtaacgt ataccgcagg tgagaatgag gatacccgga aatggacggt
    12481 atttgctatg gatacggacc gttataccta tctttacggg ttctgtgagg cggtggatac
    12541 ggaagaagat atggatgaga tttaccaaag tattttttcg cagctgtatc tgagtgatga
    12601 aaattaggga ggggtgacac atgaagaaaa aaatgccgta tgttctggcg ggaatattat
    12661 tgatgatcag tttggtgtgt atggcctgcg gcagcacaca ggaggcaact gcattaccgg
    12721 atgacgcaaa tgaaagcatt caggatgagg aagatgagga tctgcagaat gatccgaacg
    12781 atggcctggg cggtgatctg aatgaggaca cagtaaatga tttcagcggc tttgagggta
    12841 tctggctggg agaagcgaac aacgactacg attacatgga gtttgatagc gaggggaact
    12901 ggacccttta cttaagcgga gaggtcatgg atgaagggta tcttcgttat gagccggagt
    12961 gggaagctat ttatgcctac agcaaccggg atgactccgg cagccggatc gctatggaag
    13021 aagggcagct ttacagcgca gcctatggtt actttaaccc gggcaagggt atggagtatc
    13081 tctggtataa agatggagga gagtttacgg aagatgatga gccggttgcc tatgtggaaa
    13141 acggatcggg tggaagtgga aatcagaacg cacatccgga ttcctactgg agctgggatt
    13201 cggatctttg ccagcggaat gtttctgaat ttgagggcgt ctggtattat gacggcgatc
    13261 tctcggcaga gatgtatgtc gtgattgatg gaaatggaga ctggagttat taccagcgcg
    13321 ctccgggtgc tgaggctgca gaaatggatc atggcatatt cacttattcc acggatgaaa
    13381 caagtaccta ttatgcggat tctaccgtat atgatggcgt atcctacaag gtattcgact
    13441 ttgacagcga tatgctcata tggggagatg aaggcgctta ttaccggatg gagtgatgga
    13501 aaggaggagc agagtatgaa gaaacgacag ataaagcgat ttttccttac tgtgcctgta
    13561 gtgcttggta ttagcctagt ggcctgcggt gcttccggcg acgtggcggg tgatgactgg
    13621 cggaccagcg gtgtggtggc cggcagcgga acgattaccc acgatgggga aagtgatgtt
    13681 ctggtcagct tcattgatga atatggagat aatctttcca ccattacgat cggggaggaa
    13741 gaccaggata ctgctgagga aacggatccg gctatgaacc tggaagacta tgtggggctc
    13801 tgggaatatc agggggagaa ccgctggctg cgcatccacg atgattccac ctgggaattt
    13861 gtaaatgacc aggatgatgt gattgaatcg ggtacgcttt gggtggaaga aaacggaatt
    13921 acgcttcact ttgatggaag cggtgacacc ttacagcttg atcttacgat cagtggcgat
    13981 ctgatggatc ttaccaatgg cggaagtctg ttcccggtag aagcgatcca gtccagcgtg
    14041 ccttatttta ccagaaatgg cctggaaatg aatgccgaag tggaacttgg tacattcctg
    14101 ttagcaaatg gcgcaagcag ctattccggg gaaggcaatg gctaccgccg gagtgactgc
    14161 tattgggaag ttacgaaaaa ggcggatgat atccataacg gcatccgtga tctgcatttt
    14221 gacgcgatct gctatattcc ggaaagtgca atttcaactg ctgcgaatgg gacggtcgtt
    14281 gtgtccagcg aactgtacga tgcctataca ggcatgtggc tgacagcggg atcgacgttt
    14341 aacaacagca gtcgcggcga caactattat cttcacaccg tcagctggag aggaaacagc
    14401 tatctgatcg aattcaccta ttccacagac tggaatttcc aggtaggtga ctggggtgcg
    14461 gtcctgacta agagttattc cgtctatatg ccggccgact acgatggcct tgtgttcgcg
    14521 gctcaggcgg aaccggatac ttaccaggaa agtgccagaa agatgcagct ggacagcatt
    14581 tctccggaag cctgtgttct ggatcttgat accttagaac cgtacagcag tctgtatttc
    14641 agcctttgcg attaaggggg agttttatga agaaaaaaaa tagagcttcc gtttcctcca
    14701 aaccgataaa gatgacagat gcagagctgt caaaaaagct gggacattat caggcaatgt
    14761 ccgtcatatg gttctgggtg ggagtggttg gcgcagtgag cggtgccatt ttctattttg
    14821 tgttgcagaa taaggcactc aaatcgctcc ttgtggcagt gctgtttttt ggcggcattt
    14881 tctgcgcgtc ctttttcagc ggtggggcac agaaaaaact gaaagtcctg atgcaggagc
    14941 agatgggaga ctttttccgc gcagagtggg aaaaggcttt cgggccggat atccacaccc
    15001 aggaaatgcg cattgatgaa ccgttcatga gggcgcatca tctgctggac ggcaaatggg
    15061 aggagtgtac cgtggagaac ttccacgagg gcgatcatgg cggcatacat ttttctgccg
    15121 ctaatgtaca gctggatcat gtgtaccaga gaggatgttc gcatgatggc tatgaaacct
    15181 gcaggaaaat ggtctttaag ggcatcgtcc ttcgctgcag gacatgggtt ccggttccct
    15241 cgcccgttct tgctaatgtg cggacggagg atagtccatg cggtgtattg acaggcaata
    15301 atatctttga ctgcagcttc tgcgtcacgg caaacagcaa gcaggatgcc gcgtgtcgtc
    15361 ttaccccgcg gttcatcgat tttttgacga agtttaatcg ggatgtggaa gggcagatct
    15421 tgagtttttg ctgggaggga aagatctttt ccctggtatt ggaaacggat ttcggtattg
    15481 caactatagc gagcagcgta gacctgagtg acctggatgc ggcgcgtcga agctacatcc
    15541 gatctttaaa ggaactgggc agcgtgctgg accgcctgat cgagggcccc gctttaaccg
    15601 acgtggtgga aagggaagaa tatggcagaa cagaaaatga cagatgagca gttatcacag
    15661 gaactgcata agcgcaatgg cggcagggct ggcggaacgc ttctttcgct gtttggcgga
    15721 accatgttgt tttcgctctg ctctcaatcg gtagctgccg cagccggcac acaaacagga
    15781 agcagcataa ccgtaggtac aggcaatctg tgtgaacatc acccggagca tgacgatgag
    15841 tgcggctgct ctgaaggaac aacaggaaca gcctgcagtc acgagcacac agacgattgc
    15901 tatacggaag tgctcaagtg cgtccataaa catgatgatg aatgtggcta ttcccccgct
    15961 accaggggaa caccatgcga atttgcatgg gatggaaaag aaaaataaat cagccggaat
    16021 ataaatggtc ggaggtgtgt atgaaaagag ttaaaatcct ggtgtgtggg tttagccttg
    16081 cgattgcact ggtagcgttg ccgttcccgt atatagacga tatcacatcg ggcctcggct
    16141 tcagcattgc tgcagtgctt tttgcctggc ttggtattct ggagaggaga ggagcaaaaa
    16201 atggttacaa acgtgatgtg cggttatatt cagccagtac catgatgaaa gtggtgcatg
    16261 tggaagagtc tgaatatgac aggtgggagg atcagccgga tggaagttca aagctgcgcc
    16321 gcgaaaaggt atatcttcca acttacgaat attcggtgaa tggaaaaaca taccggtacc
    16381 tcagttttca gtcggtttcc agtaaacgag atgtgggaaa gcaggtggtt ggatattata
    16441 agccagaccg gccggatctc atcacggaaa accgcccaag aaagccagtg tttggtggat
    16501 tcttatattt ctacggtgct gcgatctgtc tgtttttcgg cattgtgaca tttactggaa
    16561 tgtgatgacc agatgttgat atcatatagg gcgcgactta tagtaatata ttcctgtaat
    16621 attttaatga agcattaaac aacttttttg acgaacgcac attattttgt tgtaatttaa
    16681 tgtgtgggag gacgattttt accttttgca gcgtaagtac ggacgtggaa tctattccca
    16741 cgataccgca ctgtacctgc tgggttattc ggaccgtatc ccggcgaaat atacgatgac
    16801 ctttccgaaa ggctacaatg cgccgtctct gaagctatga aacgctatgc ggcctccaaa
    16861 gacaaaaata ttcacaaact gatgaagtac gcagatcagc ttcgcgtgaa gccggtaggg
    16921 atctcatatg gatggaaact cttgcacgct ttaacgtaaa gtatcgacat ttgccatcag
    16981 aaagatttgc tgatgaatat gcaattcggg aattatcgca atacctgtaa aatatgcgga
    17041 tgcggcagcc tttatcgatg gtcgtatgaa ttttggcata acgcttgata tattagctaa
    17101 tttaacatat aatagaatta gctaataaag agaggtgatt ttatgattac tgctaccgca
    17161 acagcaatgc agaataattt tggtcgttac ctgaaccttg tgatgtctgg acaggaaatt
    17221 atcgtaacaa agaatggaaa agaagttgga aggtttattc caaaagaggc tgctgtatct
    17281 tacctgacag attctcttac aggaattcta aaagaaaaaa atgacctggc taagattaaa
    17341 gaggaggatt tgaaagcaaa gtatggtatt gttgattgat gccaatatac tactggatgt
    17401 tctgatgaat cgccaggaat ttgtaactga ttcttccatg atttggaaac tctgtgaaac
    17461 tgaacaggca aaaggatata tttccgtgct gacctatgca aatataatgt atataatgag
    17521 aaaacagtta acaccagaaa agattgagga agtatttcat aagctgaatt tgatttttga
    17581 gtttacagat tttagtccga tgattttgga aaaatccgtg aatatgaaat ggccagactt
    17641 cgaagatgcg gtacaaagtg caactgcgga agcgattcat gctgactata tagtaacgcg
    17701 aaatataaaa gattttgtga aaagcaaggt tgttgcattt acaccgggcg aattgttggc
    17761 aagattataa aaatattgat aaaatagagc aagggcggaa agatgcgtcc ttgcttttct
    17821 aatcctttgt gatatgtgtg ttccacagaa gccctattta agtttggagc tggaatttca
    17881 tatttttgtg gaaaatgaga tcaggactgc tttagcgctc ttggcaggtt ccagcaataa
    17941 tgagtagcca aaagccggat ggcaatgacc aaagcagcgc ttgtgatcat ggcaatatca
    18001 ttattcacag ggctggaaag caggaaggaa taacagatcc cacctgcaat ggaagcacat
    18061 gcataaacat gttttttcag gatataaggg gtctgccttg ccataatatc ccttaaaagt
    18121 ccgccgccta caccggtaag cacacctaag aaaacggaga gaaaatgata atccccgtat
    18181 cctgccatta cagccgtatc aatcccggtt actgtaaaag cagccagtcc aatggcatcg
    18241 aagatattca tcagcttttc ataggtgacg atacagtcac cttccaggaa atgctggttc
    18301 atacggatga cgataaaaag gaccatgaca gtaataaatg ccatgatcgt gtagatcggg
    18361 tttgtaaaga ggctgggagg aacgatccct aaaaggatgt ccctcatcat accgcctcca
    18421 actgccgttg taacacctaa tacaatgatc cccaaaagat caaggttctg ccggatcgcc
    18481 accattgcgc cggaggacgc aaaggcaatg gtacctacag cctcgatgag aaagaagatc
    18541 gaaatagaat tccccataaa tgatcggctc tcctgtgatt acagtaagta aattttgtgc
    18601 ttcttgcagg tgtcacgcat ttctttaggc cagatacttg cctgtacttc tccgatgtgg
    18661 gctctgtcaa gtaaaagcat gcacagacgg gactgtccga taccgccgcc gatggtatat
    18721 ggaagctcgc cgtttaaaag cattttgtgg tatggaagtt ccatacggtc catgcagccg
    18781 gatttttcaa gctgttctct taaggaaact tcatcaacac ggatacccat actggagatc
    18841 tcaagagcgc agtttaaggt tggataccag aaaagaatat caccgtttaa tttccagtca
    18901 tcatagtccg gtgcgcggcc gtcatgcggc tttccgcttg ccagtttgtc accgatctgc
    18961 attaagaata cgcagccgtg ttctttggtg atcaggtttt cacgttcctt tggtgttttg
    19021 tccggataca ttgcttccag ttcgctggtg gtgataaaga aaatatcatc tggaagctgg
    19081 tttacagcct gtggatattt gtaccatact tcatgctgca tgtgcttaat gatcttaaag
    19141 atcaggcgta cagtatcctt taaggtcttt tcattgcggt cttctttgtt gatgactttc
    19201 tcccagtccc actggtctac gtagcaggag tggagattgt ccagttcctc atctctgcgg
    19261 atcgcgttca tgttggtgta aagaccttca cctggctgga aaccatattc ttttaatgcc
    19321 atacgtttcc actttgcaag ggagtgtact acttccatag tctcaccggg gatagcagcc
    19381 atatcaaaag acacagggcg ttctacgccg tttaagttgt cgtttaaacc gctgctcttt
    19441 tctacgaaca gaggtgcgga aatacgctcc agatgcattt cgcgtcccat ttctttctga
    19501 aaggtatcgc ggatatattt aatggcatcc tgggtctcac gtatatccag acggggatca
    19561 tagtgttccg gtataatcag ttccattggt attttcctcc tgagaaatgt tatgtactcc
    19621 cggaaaatgg gagaaacata caatacgtac atggtaacat tattacaaag aagcttcaag
    19681 tgaaatttga taataatggc aaaggtttta tgaaaatttg ttcctgtaaa aagtgccata
    19741 tggcattttt ttatttttcc tttttgtgta tactgatgtt agagacagaa ataaagcaga
    19801 aaatgtataa aacatgggaa atcaatggga ggaagtataa acgatgaaga agttcaaacg
    19861 tatttttgtg atcgtaatgg attcttttgg cgcaggccat gcaaaagacg gtgctgccta
    19921 tggggatgac ggggcagata ccatgggcca tattgcagaa aaacatgata actggaagat
    19981 cccaaatctg accagactgg gacttgcaaa tctccatcct ttaaagggag taacaccggc
    20041 agaacatcct ctgggctatc agctggtatt aaacgaaaaa agctgtggta aagataccat
    20101 gacaggacac tgggaaatga tgggaattta taccacaaag ccatttgtta cctttacgga
    20161 tcatggtttt ccgccggaac tgatccatga gctggaagag cgcaccggac ataagatcat
    20221 tggaaataag agcgccagcg gtacagagat cctggatgag ctggcagagg aagagattgc
    20281 aaccggtcac atgatcgtgt atacatccgc tgactccgta cttcagatct gcggaaatga
    20341 agagactttt ggactggatg aactttaccg ctgctgtgag attgcaaggg aactgacctt
    20401 aaaagatgag tggagagtag gccgcgttat tgcaagacct tatgtaggaa agaaaaaggg
    20461 tgagtttaag cgtaccagca accgtcatga ctatgcttta aaaccaacag gaaagactgc
    20521 cttagatgca ttaaaagaaa agggatatga tgtgatctct gtaggaaaga tccgtgatat
    20581 ttttgacggc gaaggcatta cagaggcgta taagtcaaag agctcggttc atggtatgga
    20641 gcagaccatt gatattgcaa aaaatgaaga ctttacaggt ttatgcttta tcaacctggt
    20701 tgactttgac gcgctgtggg gccacagaag aaatccggaa ggttatgcaa aagaagtgga
    20761 aaactttgat gtgaagctgg gtgaactgtt acaggatctg aaagaagatg acctgttaat
    20821 gatcactgca gatcatggaa atgacccgac ctggaccggt acagaccata caagagagca
    20881 ggtaccgctt ctggtttatt ccccgtcttt aaaagaaggc ggaaaattgc cggaaacaga
    20941 taccttcggt gtgatcggcg ctacagttgc agataacttt gaagtgaaaa tgccggaagg
    21001 aaccatcggt acttctattt tagagcagtt taaataaaga ttataaatgt tttgaagcag
    21061 gcagagggga attctgcctg cttttttgaa attatctttt ttatgcgggc gtttgccggt
    21121 gtttataaac agtttaattg aaatgaaagg ctgtctgtgg tattatgttg attgacataa
    21181 tttgcaggaa ggcagaaaaa tccatggaaa atacaaaagc agaggtcaga gaaaagataa
    21241 aagaggctca gaaagtcctg atcgggatcg ggaaagaatg ggcgctgcgg gatgatgaaa
    21301 aagatatccg tttccgtcat ttaagcgatc cggcccagtc tgatctgaag gcagcttatg
    21361 aggaacttta tcagctgatc caggataaag attattatat tgttactact ttaacagatg
    21421 gagccattta cgaaatgcct tttgataaaa acaggatcgt agctccctgt ggaaatatcc
    21481 actggagaca gtgctccaaa gcctgcacaa aggatatctg ggaagaagga gaagtaccgg
    21541 atgatatctg tccacactgc aaggcgcctc ttaccggaaa tactttaaag gcagaaacat
    21601 atattgaaga aggctatctt ccaagatgga aagattatat gaaatggcag accggtacca
    21661 ttaaccgttc tctggtaatc ctggaactgg gagagggatt ttctacgcct acggtaatgc
    21721 gctggccttt tgagaagatc atttatttta atcagaaatc aaaattatac cgtatcaatg
    21781 aaaactttta ccagcttcca aaagaggcag aggaaagagg cgtttctgtc catgaaaatt
    21841 cagttcgctg gatgcttgaa aaataaaaaa cggaaaatgg cagataaact gcatatacag
    21901 acaaaagacg aaagagagga acgactatga tcgctgtaat agactatgac gcaggaaatt
    21961 taaaaagcgt agaaaaagcg ctgatctttc ttggagaaac accagtcatc accagagaca
    22021 gagaagaact tttagctgct gacaaagtga tccttccagg cgtaggtgcc tttggggatg
    22081 ccatggaccg tctgcatcag tatggactgg tggatgtgat aaaagagatt gtaaaaaaag
    22141 ggattccttt tcttggcatc tgcctgggac tgcagctgat gtttgaaagc agtgaagaaa
    22201 gtccgggagt agaaggtctt ggacttctac cgggaaagat ccttaagata ccggagacgc
    22261 cgggaatgaa agttcctcat atgggatgga attctttaaa gatcgatccc aactccagac
    22321 tgtttaaggg aattccagat ggaagctatg tatattttgt ccattcctat tatctgaaag
    22381 caggatctga agatatagtg gcagcaacaa cagagtatgg aacccatatc catgcagcgg
//
LOCUS       DBMDUA010000022        21031 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1573_length_21031_cov_2.962255, whole genome shotgun sequence.
ACCESSION   DBMDUA010000022 DBMDUA010000000
VERSION     DBMDUA010000022.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 21031)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21031)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21031
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1573_length_21031_cov_2.962255"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(44..1135)
                     /locus_tag="ACZPCL_03215"
     CDS             complement(44..1135)
                     /locus_tag="ACZPCL_03215"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HIR1161530.1"
                     /translation="MKEDNVLQVYYEEKRVGILALTADHKAAFQYDEKWLENGFSISP
                     FSLPLKKQVFLPTKDYFSGLFGVFADSLPDNWGRLLLDRLIRAHKQAPDELTVLDRLA
                     IVGKSGMGALTYYPEKSFSEQTGNLDLDELAEECQKILNTEYSDKLDELYRLGGTSGG
                     TRPKIMTTINGEEWIIKFPAHVDGKDTGKMEYDYSCCARQCGIVMSETRLFPSDKCKG
                     YFGTKRFDRRPDGKRLHMLTAAALLELDFEQPSLDYHSLMKLTKILTRDNAEDMENMF
                     RRMCFNVFAHNRDDHSKNFTYLYDDKEDKWHLSPAYDLTYSNTYYGEHTTTVDGNGRD
                     PGRKELLAVGIAAVHSAHDRERYARGGDG"
     gene            complement(1122..1400)
                     /locus_tag="ACZPCL_03220"
     CDS             complement(1122..1400)
                     /locus_tag="ACZPCL_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1161531.1"
                     /translation="MEQLIWETAEELDQKLAQRVRNIRKRRLISQEKLASLSGVSYGS
                     IKRFEATGQISLISLTKIAMALDIADELRNIFTQVPYRDIQEVINERR"
     gene            complement(1615..2469)
                     /locus_tag="ACZPCL_03225"
     CDS             complement(1615..2469)
                     /locus_tag="ACZPCL_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008753830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1161532.1"
                     /translation="MKHTVTLRNGVQVPALGLGTWFLGENKAARAREMESLRTGISAG
                     MTLIDTAEMYGNGKAESLIKEVISDKKRDDLFLVSKVLPNNAGKHRLEQALDNSMKRM
                     GVEYLDLYLYHWRGNYPLEETVAELDRVKKAGKIKEWGVSNFDIDDMEELWQIPEGRN
                     CLVNQVLYHTGSRGIEYSLLPWMREHDVALMSYCPLAQAGTLREGILNNPVLKEIAKK
                     YNATVEQVMLAWNIRDGHTIAIPRSGRAEHTLLNAQADQIQLTEEDYKAIDQAYPPPV
                     RKEYLDIQ"
     gene            complement(2471..3370)
                     /locus_tag="ACZPCL_03230"
     CDS             complement(2471..3370)
                     /locus_tag="ACZPCL_03230"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR1161533.1"
                     /translation="MNTDFIKGVIVPIITVIDEEEKINEAAMRAQVDYVINGGLHGIL
                     AFGSNGEFYQIEEEEMERGFKIMVDQAAGRVPVYFGIGAINTKKCVRLAKMAVANGAA
                     GISILQPMFLKPNFNELFLHFKTIAEAVPEIPVLLYNNPGRVGYTLSADLVEKLAHEV
                     KNIVGMKDTSGDITQTEEFIRRNADVGFKVFGGKDTLLYASLCIGAVGGVCTAGNFMP
                     ELIVDIYNKYVAGDLKGSLAAQYKLNPVRLAMDGASFPVAAKDMAIMRGQAAGKPYLP
                     NLPTPEGASYDKMKKEMEKAGLI"
     gene            complement(3399..4292)
                     /gene="garR"
                     /locus_tag="ACZPCL_03235"
     CDS             complement(3399..4292)
                     /gene="garR"
                     /locus_tag="ACZPCL_03235"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HIR1161534.1"
                     /translation="MKVGFIGLGIMGKPMAKNLLKAGYQVCVNDFHKEAVEELVAAGA
                     TAGANNAEVAKGVDVVITMVPNSPNVRAALLGENGVAEGADKDTVVIDMSSIDPVESK
                     KIAAELKERTGMEMLDCPVSGGEPKAIDGTLSIMCGGKKEVFDKYTDLLKAMGSSVVY
                     VGEIGSGNVAKLANQMVVAANIGICAEAMTFAKKMGTDPELVYQAIRGGLAGSTVMDA
                     KVPMMLAGNYKPGFRIDLHIKDLNNALNASHAINMPVPMTAHMMEVMQELKNHDEGSC
                     DHDDIIRYYERMTGVSIVKEK"
     gene            complement(4337..5377)
                     /locus_tag="ACZPCL_03240"
     CDS             complement(4337..5377)
                     /locus_tag="ACZPCL_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HIR1161535.1"
                     /translation="MGKFAPEQGCIELYDQEGACAHLSVGPGFITDILPMILNGEEHS
                     YNEWRNSLYWKIRNAGFQSEKAVEVGQLDLMMLDILAQRANKPLHRFMGATKDWAQAY
                     KGGGSLLMEDDELVEDMVRYVEEGYTTVKFKVGSNDGTDMERDLRRIEKVRKAVGDKI
                     GVAVDCNQRWDVDSAYKFAKLCEPYHLAWLEEPIHSHNMNGIRRLKEMGVKIPIAFGE
                     SMRIYYEYETYIEKGVDHVQPSVGRMTRMDDLIRIRDLAREKGVKFTSGGRIYLNAIF
                     GCLYNEDEWIEYHEPISRPVGAYTLFQPEEKNGRFYCQPDLPGNPQRLDIAKLEKDGL
                     MESREIYYPKNW"
     gene            complement(5522..6898)
                     /locus_tag="ACZPCL_03245"
     CDS             complement(5522..6898)
                     /locus_tag="ACZPCL_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1161536.1"
                     /translation="MKKNVKKLGALLLAGAMAVSTVGCMPTSKPQESTGEQNAQASDA
                     TQGTEEPAAPEGLEVDTQTPITLRLNWWGGDSRNQATLAAIDKFQEKYPNIKVAAEYE
                     AFNGHEEKIALGIKSGNAADVMQLDWSWVSTYSPKGDNFYDLNKVSNILDLDNYTEGD
                     KSVFTINGKLNAIPISNTGRVFCWNKTTFDKIGVEIPTTLDELLAAGKAFEAYDDSYY
                     PLVTKELDRAFLMVYYLQCKYGKDWVKDGALQYSQEEIAEGFDFLKNLEDNHVIPTLQ
                     KVAGDGADLIDTNANWIDGHYAGIFLYDTSIVKHAEAVKDGEFVIGDYIKMGDYHGGF
                     IKVNQAFGISATTEHPAEAAALIQFLVAEEDGVKTLGDTRGVPANKAGLSMLDLSGSQ
                     VAEANAKVMEWSSFQMDPTFERNSFTGADGTFFNALQLSSYGESDSMSCAKIVEDGVA
                     NELSSTGL"
     gene            complement(6920..7843)
                     /locus_tag="ACZPCL_03250"
     CDS             complement(6920..7843)
                     /locus_tag="ACZPCL_03250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1161537.1"
                     /translation="MRKGRIIDSHAHLVQYIAGTGAGGELRSIGNGMAVYANGQTVRM
                     IPKEFGGDQVTPEHLIQVMDKNGVEKAVLLQGNFYGYQNFYTHLAVKKYPDRFAGAGA
                     YDPFSRDKDGIRRYLFEELGFKIEKFEVSTGSGLMAIHPELKLDGPVMDEACSYACDH
                     GHVFVIDIGKCGSDSWQVPELCNLVKRYPEMKFVACHLLAPSNKDKDRLRWALEQLQL
                     PNLWFDLSSVVHNCRPDEYPYPKAIEFIQLAKEMVGAEKLMFGTDFPSALKEDVYGHY
                     VGYIADSTVFTDQEKEQIFYENAYGVYFNHH"
     gene            complement(7840..8688)
                     /locus_tag="ACZPCL_03255"
     CDS             complement(7840..8688)
                     /locus_tag="ACZPCL_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1161538.1"
                     /translation="MSRETRNKVNMVWRYVILLAVGVVMVYPLVWMIGASFMTNEQIF
                     SSVSCIPRFPTLSGYKKGFEGYQGMTLLHFMVNTYKFVIPKVLFTVASATLTAYGFAR
                     FNFIGKRFSFAVLLSTLFLPQVVLNVPQYILFDQVGWLDSYLPIIIPSLLAGDTYFVY
                     MLIQFLRGIPKELEEAAEIDGCGIMQRLWYVIVPMLKPSIVSCALFQFMWASNDFMGP
                     LIYVNTVAKYPVSIFLRMSMDADVGFEWNRVLAMSLIAIVPSLIVFFTAQDSFVEGIA
                     AGGVKG"
     gene            complement(8694..9584)
                     /locus_tag="ACZPCL_03260"
     CDS             complement(8694..9584)
                     /locus_tag="ACZPCL_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1161539.1"
                     /translation="MKFKEFRKKNVGLLLILPWWIGFAIFKLYPFISSLIYSFHDYNL
                     FRTATFSGLSNYKKILGDPLIMKAFIQTFKYAFLTVPLELIFALFIAYILNFKIKGVN
                     FFRTAYYIPSILGGSVSIAVLWRFLFKTEGLVNMIASRFGIQPFNWLGDPGGAFWVIV
                     FLKIWQFGSPMVIFLAALKGVSKDLYEAASIDGAGKWRQFFSVTIPLITPVIFYNFVT
                     QLCNKFQEFNGPFIVTQGGPLRSTTLVSLLVYNNAFKSYEMGMASAIAWLLFLIIMTL
                     TGVAFFSQKYWVYYSDEDGR"
     gene            9906..11810
                     /locus_tag="ACZPCL_03265"
     CDS             9906..11810
                     /locus_tag="ACZPCL_03265"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1161540.1"
                     /translation="MTTRQFYYLTVIADLGNLSAAAQKLQISQPALSKFLTEYEASLG
                     FQIFLRYHRHLAPTSVGRCVIEYAHKIVSEQNRLSQSLRTIINRDHACIRLGTTPNRG
                     SVIYSRIYQTFMDRYPDISLSLTERYSNEQTEAVLKGQVDLTIGSGKSSDKVTDIPVA
                     HEELLVALPAAHPLASSDKVRLSDLKDTPFVLQGPRHNIRILAEELFKEAGFEPVITF
                     ESDNVIIVDSMLHHAAGAGFVSKKYAVPCNELVFLPLDPPVYQETHIRFPLGHKLTEP
                     EQYLAFLLIRDIQSNPEQRFEIIPSKEVNDLLQAADPDMSSKNDPASIQGGHAAASSV
                     QGYSELNLNPQLLRYVIAIVDEKSLTKAAEKFYLTQPTLSRYLHGMEKMLGARLFTRE
                     HNKLLPTNAGKVFVNFARNILQIEEEMSEHIKTYQQGHDGGIYLQCDPSLLHIIQTQV
                     IPSFKKQHPDIQLNVSTNSREQIQESLLNASADYGVYFSCEEEHPILDSRILDEDELV
                     YCSKHDLLSDPLEHTKIMTVPKGNPLYAEQKRLFQQLYLTFPATSCEAAPSILFFLEK
                     NGIGNTILPSKTMANNPDDRHFLTPAPGYFLIMAHHPIRTMPPVTRDLETLLYDTFET
                     YFEHFAELFS"
     gene            complement(11759..12160)
                     /locus_tag="ACZPCL_03270"
     CDS             complement(11759..12160)
                     /locus_tag="ACZPCL_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014766.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161541.1"
                     /translation="MFIETDTELTGIGEAATFGSSMDAMEFVVEKQLKSLLLDEDPTK
                     IEFLHQKMMWNNWANGRQGMVMGAISGIDVALWDILGKVANLPVCKLLGQVSDKVQGY
                     AAAFLIAMHLHFFHIYENNSAKCSKYVSNVS"
     gene            complement(12150..12398)
                     /locus_tag="ACZPCL_03275"
     CDS             complement(12150..12398)
                     /locus_tag="ACZPCL_03275"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR1161542.1"
                     /translation="MIPTSIGLIQYGVATGTSISDLFMAGFLPGIFICLVLCVVAHFL
                     CKKQGFEPSERAATEEKVQAFKDAILALLMPVIISYLH"
     gene            complement(12359..12760)
                     /gene="dctP"
                     /locus_tag="ACZPCL_03280"
                     /pseudo
     CDS             complement(12359..12760)
                     /gene="dctP"
                     /locus_tag="ACZPCL_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015445.6"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
     gene            12784..13095
                     /locus_tag="ACZPCL_03285"
     CDS             12784..13095
                     /locus_tag="ACZPCL_03285"
                     /inference="COORDINATES: protein motif:HMM:NF019349.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HIR1161543.1"
                     /translation="MTFEARLLMEPYIIENYSKYIDMDKLLDLEEQTKKILSSDILEQ
                     KDAVVFCNIDDALHRTIADACKNKYLNMNLSQIYDQNIRIRILGEQPNNSFKGCCKTD
                     K"
     gene            complement(13258..14148)
                     /locus_tag="ACZPCL_03290"
     CDS             complement(13258..14148)
                     /locus_tag="ACZPCL_03290"
                     /inference="COORDINATES: protein motif:HMM:NF016852.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HIR1161544.1"
                     /translation="MLREKPEFYKEEVRCGYTVNEKTKRVWAVQLEMLDEVERICKAN
                     GLKYFADSGTLIGAIRHEGYIPWDDDIDLVMLREDYDKFVQIAPKWLKEDLVLQTVYT
                     EKNYLRGHAQIRNSKTCGCNEEDKKAGYNCGIFIDIFPLDGMPKTEKERKNWAKKIQF
                     YWTVLYTWYRFDYYENATVFGKLLHGIGMLLHVPMVKAYKGYEKLCTRFKGKDTEYVC
                     DTVFIRYLEKNTWKREWFKEAVYMPFENRKIPVPVGYDGRLTAEYGDYMKPAKAPTMH
                     GGLILDPDVPYTEYLKKENK"
     gene            complement(14208..15692)
                     /locus_tag="ACZPCL_03295"
     CDS             complement(14208..15692)
                     /locus_tag="ACZPCL_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014051.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR1161545.1"
                     /translation="MEYREELMAKAPSVRKNYIYNLIYQVMTLITPFITTPYISRVLG
                     ADGTGVQSYTNSVVQYFAILAALGTASYGQREIARHRDEIKIRSRLFWEIEVLCMATT
                     AACLIIWLFVIGFAREYRPYYVVLTMTLLAVAFDISWFYGGLEQYSLIVLRNTAVKLV
                     GIAMLFLFIREKEDLLLYVALTAATGLLGNVSMWGYLKGQVEKPVLKELRPLRHLKET
                     LVYFIPTIATSVYTILDKTMLGWFSGENKSQNGYYEYATGFVNMAKILIMSFNAVMSA
                     RMSYLFGTGRMEEIHKRFQDSLDFVLLMAMPIMLGLAGIAAQFIPWFLGNGYLPVTFL
                     MYVCSPLVLVVGLSDCIGSQILTPGGKRAQSGKVIVAGAMVNACLNFLLIPHFAALGA
                     AAASVAAECFITSLYLWLGRDFIKLKMLVRYGWRRLVAAVIMLGAVVWTGQFLEQVRG
                     LGPITTFIQIGAGAMVYFLVLLVMRDPFLYQYLGKIYRRLTGQP"
     gene            complement(15834..16589)
                     /locus_tag="ACZPCL_03300"
     CDS             complement(15834..16589)
                     /locus_tag="ACZPCL_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008755204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HIR1161546.1"
                     /translation="MKLLFDLHTHTVASGHAFSTLKENIEEAAAKGLKAMGTSDHYSA
                     MPGSAQPIYFTNFKAIKEKILGVRIFTGMEANIIDLEGKLDAEEAVLKKMDYVIASLH
                     VPCVKAGTREENTNALIGAMKNPYVKIIGHPDDDRFPLDYEKLVKAAAKEKVALEVNN
                     SSFSPRTGRLNADKNLPVMLSFCKKYKVPVIVDSDAHIFYDIGNLDRAKEMLEACEFP
                     KKLILNTKLKGLSYVLNLEREETREKYDHMMED"
     gene            complement(16607..17638)
                     /locus_tag="ACZPCL_03305"
     CDS             complement(16607..17638)
                     /locus_tag="ACZPCL_03305"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1161547.1"
                     /translation="MSLYLEHITQRFGQTLALNDIDLEIRNGEFLAILGPSGCGKTTL
                     LRAIGGFAKPTEGKIRMDDVIYSGNGKMVPVEQRDLGMVFQSFALWPNMTVRQHVEFP
                     MKSPKQKHLTEAQKKEAVDAAIENMGLKPYEDRYPGELSGGQRQRVSLARAIVGKPSI
                     LLMDEPLSALDAELKISMRRVIQDVHRMTGATIIYVTHDQSEALAMADRILIMKNGKI
                     EQVGTPEEIYLHPQTEFAASFVSKSNFVRGQWNDSYFKVMDQSLIYDGPEVAQAFKNH
                     GLFPVRPEQFHIVKEGKGIPATVTNRQYSGREIHYSLKCGEDTFTVYAGSREQYEPGE
                     QVKLVYQAS"
     gene            complement(17583..19313)
                     /locus_tag="ACZPCL_03310"
     CDS             complement(17583..19313)
                     /locus_tag="ACZPCL_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161548.1"
                     /translation="MKHKKVLGSTGLQLFYLGVFLVFIAPIVRLLFMSLKGSDGYGLS
                     NYQVLLQEKRTREAIFNTILIAASSTMIAIVAGSGLALAAAYTNIKRKRLLELLVLLP
                     FIIPSYIITLSWSGFLSKKGIVNQILGAVGIGPVNIYSIGGILLVIGICNIPVVYMSV
                     IHMLRRIPRDLEWASRACGFGLSYTLVHVDLVQVLPAIIAGGLLAFLSAIDNFSVPAF
                     LGISSGIPVLSTYIYEKAIGFGPDAFPLAAALSVLLSVIAVGGTLMETALVGKNGKSE
                     SIKEDYSVRIELEGPKRRLLEWGLLGTLGVLNLVPILVMIKSAFLKTYGLKMTLDNLS
                     LKNFAFVFTNRGVLAAVRNSILLALVTCGVCIVVGTAAAYMKVRRNSKGAVTMEKCAS
                     LTYAIPGIVLSLAMIFHWVEPLPGIRPGVYGTIWILVIAYITRYLILQIKESTTALMS
                     VNPELEEAVRACGRGKRALWQQVLLPMLVRPILSGSFLIFVSSLTELTLSSMLASAGT
                     KTIGLTIFNFQQSGDYNLSAAMSAVIVVMVLTGYCLVALKPAEKKKRGKEHESLFRAY
                     NPEVRANTGA"
     gene            complement(19349..20440)
                     /locus_tag="ACZPCL_03315"
     CDS             complement(19349..20440)
                     /locus_tag="ACZPCL_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024808.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1161549.1"
                     /translation="MNKKMALILTTAAVMGLAGCSGQTDKTETTAAPAKTTAEPATQA
                     KAETTEASTEAEKKDVSGKITLYTSQPEEDAQKLIDGFNKECPDVTVDVFRSGTEEVV
                     SKVLAEQTAGAVQADVLLVADSVTFETLKEQDMLLPYESPELKGIPEEYIDKDHMYTG
                     TKVITTGIAYNTDLVKDAPKGFADLTGEAFKDAVIMPSPLYSGAAAYNLGVLSRNETL
                     GWDFFQGLKDNGITVDKGNGAIQKAVVAGEKSCGILVDYMANRSKNDGAPVEFVYPEE
                     GSPAITEPIGVLKDSRNQDAAEAFVDFVLSDEGQELAASIGYTPVKEGVAAPEGLKSI
                     DQIKTISADTKELYSSRDADKEKFSEIFQ"
     gene            complement(20443..>21031)
                     /locus_tag="ACZPCL_03320"
     CDS             complement(20443..>21031)
                     /locus_tag="ACZPCL_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR1161550.1"
                     /translation="YSWDLNLASMNQGIREMSIKQVEASMRLAAELEAMELTVHPGHM
                     TLPGRREESMKILQDSLEQIGRLSDRIGVDVSLEIMEKIPKEFVTDAQSMEEAAGSRK
                     EQFFYTLDIAHCDSEEEPFHILDQMKRISKIHISNRKGKCYHTPLENGDFSFKEMLPQ
                     LLAYGLPLVTEGYDDSRDFHTFTENTAFLKTNGGL"
BASE COUNT         5573 a         5242 c         3895 g         6321 t
ORIGIN      
        1 tttgtcccgg gcttgctcgg catcctggtg gagcctgacg gtttcagcca tcgcctcctc
       61 ttgcatacct ttctctgtca tgcgctgaat gtactgccgc aattccaact gccagaagtt
      121 cttttcttcc cggatctctt ccatttccat caaccgtagt tgtatgttct ccataatagg
      181 tattgctgta ggtaagatca tacgcaggac ttaaatgcca tttatcttcc ttgtcatcat
      241 ataaatatgt gaaattcttt gaatgatcgt cccggttatg ggcaaataca ttaaaacaca
      301 tcctacgaaa catattttcc atatcttctg cgttatctct tgtcagtatt tttgtcagct
      361 tcatcaaact atgataatcc agacttggct gttcaaaatc taactctaaa agtgccgccg
      421 ctgtcagcat atggagcctt tttccatctg gtcttcggtc aaatcttttt gttccaaaat
      481 atcctttgca cttgtcagaa ggaaataatc ttgtttcact cataacaata ccacattgtc
      541 tggcacagca ggaataatca tactccattt ttcctgtatc tttcccatct acgtgggcag
      601 gaaattttat gatccattct tccccattga tcgtagtcat gatctttggt cttgttccgc
      661 cactggtgcc acccagacgg tacagttcat ccaacttatc tgaatattct gtattcaata
      721 ttttctgaca ttcttctgcc agttcatcta aatccaaatt ccctgtctgc tcagaaaaac
      781 tcttttcagg ataataagtc agcgctccca ttcctgactt tccaacgatt gccaatcgat
      841 ctaaaactgt cagttcgtct ggagcctgct tatgtgcccg gatcaaccgg tctaacaaca
      901 atcttcccca attatccgga agactgtctg caaacactcc aaaaagacct gaaaaataat
      961 cttttgttgg taaaaatacc tgttttttca gtggaagtga aaaaggactg atagaaaaac
     1021 cattttccag ccatttttca tcatactgaa aagccgcttt atgatccgct gtcagcgcta
     1081 gaattcccac acgcttttcc tcatagtata cctggagcac attatcttct ttcatttata
     1141 acctcctgga tatctctgta cggtacctgc gtaaaaatat ttctcagttc atctgctata
     1201 tccaaagcca tagcaatctt cgtcaaagat atcaatgaga tctgtcctgt cgcttcaaat
     1261 ctcttgatcg aaccatagct tacaccgctt aaagatgcta atttctcctg ggagatcaag
     1321 cggcgtttcc tgatattacg cactctctgc gcaagtttct gatccagttc ttctgctgtt
     1381 tcccatataa gctgttccat agacacccca tttatctttc tgctaatatt ttatccaaaa
     1441 tattagtttt atattaaaaa cagataatat tttatctatt tcattttatc acttattttt
     1501 gttgcataca atatattttt cacctaatta ctgataagca acaaaaaaga caactgcgtt
     1561 gctaaaaacg tcagttgtcc ttttatgatc ttgttcaaat attaatgcga aattttactg
     1621 tatatccaga tattctttcc ttactggcgg cggatatgcc tggtcgatag ctttataatc
     1681 ttcttctgtc agctggatct gatctgcctg tgcattaaga agtgtatgct ccgcacggcc
     1741 gcttcttgga atggcaatgg tatgtccgtc ccggatattc caggcaagca tgacctgctc
     1801 tacggtggca ttatactttt tagcgatctc ttttaaaacc gggttgttta agatgccctc
     1861 tcttaaagtc ccagcctgtg ccagaggaca ataggacatc aaggccacat catgctctct
     1921 catccacggc agaaggctgt attcaatgcc tctggagcct gtatgataaa gaacctggtt
     1981 taccaggcag tttctgcctt ccgggatctg ccacagttct tccatatcat caatatcaaa
     2041 gttggaaacg ccccattctt tgatctttcc tgcttttttc acacgatcca gctctgctac
     2101 ggtttcttcc agaggatagt ttcctctcca gtgatacaga taaagatcca ggtattccac
     2161 gcccatccgc ttcatggaat tatctagcgc ctgttccagt cggtgtttcc ccgcattgtt
     2221 tggcagaact ttagatacca gaaaaagatc atcccgtttt ttatcagaaa tgacctcttt
     2281 tataagactt tccgcttttc catttccata catttctgca gtatcgatca gcgtcatacc
     2341 agcagaaatg ccagtgcgca gactttccat ttcccgcgct cttgctgctt tattttcccc
     2401 aaggaaccag gttccaagcc ctaaggccgg aacctgcact ccgtttctta atgtaactgt
     2461 atgtttcata ttagatcaga cctgcttttt ccatttcttt tttcatctta tcataagacg
     2521 caccttctgg tgttggaaga tttggtaaat atggttttcc agctgcctgt ccacgcatga
     2581 tagccatatc ctttgcagct accgggaagc ttgcaccatc cattgccaga cgaactggat
     2641 ttaacttata ctgggctgcc agggaacctt tcagatctcc ggccacgtat ttattgtaaa
     2701 tatctacgat cagctccggc ataaagtttc cggctgtaca cacaccacct acagctccaa
     2761 tacataagct ggcatataac agtgtgtcct ttccgccaaa taccttaaaa cctacatctg
     2821 catttctgcg gataaattcc tctgtctggg taatgtcacc ggaggtatct ttcataccta
     2881 cgatattttt cacctcatgg gccagttttt ctaccagatc tgcacttaag gtataaccaa
     2941 ctcttccagg attattatac aacagaaccg gaatttccgg aactgcctct gcaattgttt
     3001 taaaatgcag gaacaattcg ttaaaattgg gttttaagaa cataggttgt aaaatggaga
     3061 tgcctgctgc accgtttgct actgccattt ttgcaaggcg gacgcatttt ttcgtattga
     3121 tcgcgccgat tccaaagtaa acaggaactc ttcccgctgc ctggtctacc atgatcttaa
     3181 atccacgttc catttcctct tcttcgatct ggtagaactc accgttggag ccaaaagcaa
     3241 ggataccgtg aaggccgcca ttaatcacat agtctacctg tgcccgcata gctgcttcat
     3301 tgattttttc ctcttcgtca ataactgtaa taataggaac gataacgccc ttgataaaat
     3361 ctgtattcat aacatttccc tcttagatca ataaagaatt atttttcttt tacaatagaa
     3421 acaccggtca ttctctcata gtaacggata atgtcatcat ggtcgcagct gccttcgtca
     3481 tggttcttta attcctgcat tacttccatc atatgtgcgg tcattggtac tggcatattg
     3541 atagcgtggg aagcatttaa tgcattgttt aaatccttga tgtgcaggtc aattctgaat
     3601 ccaggcttgt aatttccagc taacatcata ggcacctttg cgtccataac agtagaacca
     3661 gcaaggccgc cacggatggc ctgatatacc agttctggat cggttcccat tttctttgca
     3721 aaggtcattg cctctgcaca gataccgata ttggctgcaa cgaccatctg gttagccagt
     3781 tttgctacgt ttccggagcc gatctcacct acatatacaa cagaacttcc cattgccttt
     3841 aataagtcag tgtacttgtc aaatacctct ttcttaccac cacacatgat agataaggtt
     3901 ccatcaattg cctttggctc tccgccggaa accgggcagt ctaacatttc catgccagtt
     3961 ctttctttta actcagctgc aatcttcttg gactctactg gatcgatgga agacatgtcg
     4021 atgactacgg tatctttatc tgctccctct gcaacaccat tttcacctaa taaagcagct
     4081 cttacatttg gagagtttgg caccatggta atgactacat ctacgccctt tgcaacttct
     4141 gcgttatttg cacctgctgt agcacctgct gctaccagct cttctactgc ttctttgtgg
     4201 aagtcattca cacaaacctg ataacctgcc tttaaaaggt tctttgccat tggctttccc
     4261 ataattccta aaccaataaa tcctactttc attttttatt ctctcccgtt attaaacttg
     4321 aatttttcga atttacttac cagtttttcg gataatagat ctctctgctc tccataagcc
     4381 catctttttc cagcttcgca atatccagac gctgtggatt accaggaaga tctggttggc
     4441 agtagaagcg gccgttcttt tcctcaggct ggaacagggt ataagcacct accgggcggc
     4501 tgatcggctc atggtattcg atccactcat cttcgttgta taaacaacca aaaatggcat
     4561 tcagataaat tcttccgccg gatgtgaatt taactccttt ttcacgggcc agatcacgga
     4621 tcctgataag atcatccatt ctggtcattc tgcctacaga cggctgaacg tgatctacgc
     4681 ctttttcaat ataagtttca tattcataat agatcctcat ggactctcca aaagcgattg
     4741 gaattttcac tcccatttct tttaacctgc ggatcccgtt catgttatgg gaatggatcg
     4801 gttcctccag ccatgccagg tgatatggtt cacacagctt cgcaaacttg tatgcactgt
     4861 ccacatccca tctctggttg cagtcaactg ctacaccgat tttgtctccc actgccttac
     4921 ggaccttttc gattctgcga agatcccgct ccatatcggt accgtcatta cttcccactt
     4981 taaatttaac agtggtgtag ccttcctcca cataccggac catgtcttct accagctcat
     5041 catcctccat aagaagggaa ccgccgcctt tataagcctg ggcccagtct tttgtagctc
     5101 ccataaatct gtgaagaggc ttattggctc tctgagccag gatatccagc atcatcaggt
     5161 ctaactgtcc cacttccaca gccttctcgc tttgaaatcc tgcgtttctt atcttccagt
     5221 aaagactgtt tctccactca ttgtagctat gctcttctcc gtttaagatc ataggaagaa
     5281 tgtccgtaat aaatccagga cctactgaca gatgagcgca tgctccttcc tggtcataca
     5341 gttcaatgca gccctgttcc ggtgcgaact ttcccattcc tccggttccg tctttaaaag
     5401 gctttggtag gggaatttta tggaaatgat agtatacggc ttttgtaaat tttattttat
     5461 cctcaaaaga aaaagtaaac atcttcattg cctccgttta tcttttttat ttttatagat
     5521 cttataatcc tgtggaagac agctcatttg ctacaccgtc ttctacgatc tttgcacagg
     5581 acatggaatc actttcccca tagcttgaaa gctgaagagc attaaagaaa gtaccatctg
     5641 cgcctgtaaa ggagtttctt tcaaaggttg gatccatctg gaaactgctc cattccatta
     5701 cctttgcatt tgcctctgct acctggcttc cgctcagatc cagcatagaa agtccagcct
     5761 tgttagcagg aacaccacgg gtatctccca gtgtctttac accatcttct tcggctacca
     5821 ggaactggat caaagcagct gcctctgccg gatgctctgt ggttgcgctg ataccaaatg
     5881 cctggtttac cttgataaag ccgccgtggt aatcacccat cttaatgtaa tcaccgatca
     5941 caaactcgcc gtcttttact gcttctgcat gttttacaat ggaagtatca taaaggaaaa
     6001 tgcctgcata atgtccatcg atccagtttg cgtttgtatc gatcaggtca gcgccgtctc
     6061 ctgctacctt ctgtaaagta ggaattacgt ggttgtcctc cagattctta agaaaatcaa
     6121 atccttctgc gatttcttcc tggctgtact gcaaagcgcc atcctttacc cagtctttgc
     6181 catatttaca ctgcaggtaa taaaccataa gaaaagcacg atccagttcc tttgtaacca
     6241 gcggatagta ggagtcatca tatgcctcaa atgcttttcc tgctgctaac agttcatcta
     6301 atgtagtagg gatctccaca ccgatcttat caaaagtggt cttgttccag cagaacactc
     6361 ttcctgtatt gctgatgggg atggcgttca gcttgccgtt gatggtaaat acggatttat
     6421 ccccctctgt ataattgtcc agatccagga tattggatac cttgttcaga tcatagaagt
     6481 tgtccccttt tggtgagtag gtggacaccc agctccagtc taactgcatc acatctgctg
     6541 cattgcctga tttgatgccc aatgcgatct tttcctcatg gccgttaaat gcctcgtatt
     6601 cagctgctac cttaatattt gggtatttct cctggaattt atcgattgct gccagcgtag
     6661 cctgatttct ggaatctccg ccccaccagt tcagtctcag tgtaatagga gtctgagtat
     6721 caacctcaag tccttccggt gctgcaggtt cttccgttcc ctgtgtcgcg tcactggcct
     6781 gcgcattctg ttcccctgtg gactcctgcg gtttagatgt aggcatgcag cccactgtgc
     6841 tgacagccat tgctcctgcc agaagtaatg cccctaattt ttttacattt ttcttcattt
     6901 ttgttttctc ccttctgcat caatgatgat taaaatacac tccataagca ttttcataaa
     6961 aaatctgttc tttttcctga tctgtgaaca cggtcgagtc agctatatag ccaacataat
     7021 gaccatatac atcctctttc agtgccgatg gaaaatctgt gccaaacatc agcttttctg
     7081 ctccaaccat ctccttagcc aactggataa actcaatggc cttgggatat ggatactcat
     7141 ccggcctgca gttatggacc acacttgaca gatcaaacca gagatttggc agctgtaact
     7201 gttctaaggc ccagcgcagc ctgtctttat ccttgtttga tggagccagc agatggcagg
     7261 ccacaaattt catctccggg tatcttttaa ccagattgca aagttccgga acctgccagc
     7321 tgtcacttcc acattttcca atatcaatga caaatacatg accatgatca caggcatagg
     7381 aacaggcttc atccatcaca ggaccatcca gtttcagttc aggatggata gccatcaggc
     7441 cggagcctgt gcttacctca aatttttcta tcttaaaccc cagttcttca aacaggtacc
     7501 ttcgtatacc atctttatct ctggaaaatg gatcataggc accggctcca gcaaaccggt
     7561 ccgggtactt tttaactgcc agatgggtgt agaaattctg atatccatag aaattgccct
     7621 ggagcagaac tgccttttct accccatttt tatccattac ctgtataaga tgttccggcg
     7681 tcacctgatc cccgccaaac tccttcggta tcatccttac ggtctgacca ttggcataga
     7741 cagccattcc gtttcctatg ctgcgcagct ctccacctgc gcccgttccg gcaatatact
     7801 ggaccagatg agcatgactg tcaatgatcc tgccctttct catcccttta cacctcctgc
     7861 tgcaattcct tctacaaagg aatcctgtgc tgtaaagaat acgatcagtg aaggaacaat
     7921 agcgatcagt gacatggcta atactctgtt ccattcaaat cctacgtctg cgtccatgga
     7981 catacgaagg aaaatggata ccggatattt agctactgta ttaacataaa taagtggtcc
     8041 cataaaatca ttggatgccc acataaactg gaacaatgca caggaaacaa tggaaggttt
     8101 cagcattgga acgatcacgt accagagtct ctgcataatt ccgcatccat cgatctcagc
     8161 tgcttcctcc agttcttttg gtattccacg aaggaactgg atcaacatgt atacaaagta
     8221 ggtatctcct gccaacaggg aaggaataat aataggaaga taagagtcta accatcctac
     8281 ctggtcaaag agtatgtact gaggtacgtt taatactacc tgaggcagaa acagtgtgga
     8341 cagcaatact gcaaaagaaa aacgtttgcc aataaagtta aaccgtgcaa atccataagc
     8401 agtcagtgta gcggaagcta cggtaaaaag tacttttgga attacaaact tataagtgtt
     8461 caccatgaaa tgaaggagtg tcattccctg gtatccttca aagccttttt tgtaaccact
     8521 taaggttgga aatcttggta tacagcttac gctggaaaat atctgttcat tggtcataaa
     8581 agaagctccg atcatccaga ccagggggta aaccatgaca actcccactg ccagaaggat
     8641 gacatatctc cagaccatat ttactttatt acgtgtttca cgagacattc gatctacctc
     8701 ccatcctcgt cagaataata tacccagtac ttctggctga agaacgctac acctgtcagt
     8761 gtcatgatga tcaggaacag aagccaggca atggcacttg ccatacccat ttcataactc
     8821 ttaaaggcat tgttatatac cagaagagat accaatgtag tggaacgaag aggtcctccc
     8881 tgagttacga taaacggacc attgaattcc tggaacttgt tgcacagctg ggtaacgaaa
     8941 ttatagaaaa taaccggtgt gataagtgga atggttacac tgaaaaactg tctccatttt
     9001 cctgctccgt caatggatgc tgcctcatac agatcctttg acactccctt taaggctgca
     9061 aggaagatca ccatcgggga accaaactgc cagatcttta aaaatacaat gacccagaaa
     9121 gctccgcctg gatcacccag ccagttaaag ggctggatac caaacctgga tgcgatcatg
     9181 tttacaagtc cctctgtctt aaacaaaaat ctccacaata ccgcaatgga aacggatcca
     9241 cccagaatag aagggatgta ataagcagtt ctgaaaaagt tcacgccctt gatcttaaaa
     9301 ttcaggatat aagcgataaa caaagcaaag atcaattcca gtggaacggt aagaaaggca
     9361 tatttaaagg tctgtataaa tgccttcatg atcaatgggt cgcccagtat ttttttgtag
     9421 ttgcttaagc ctgaaaaagt agctgtcctg aaaaggttgt aatcatggaa actgtagatc
     9481 aatgatgata taaagggata cagtttaaag attgcaaatc cgatccacca cggcaggatc
     9541 agcaacagtc ccacattctt ctttctgaat tctttgaatt tcattttatc gcctcctcca
     9601 tgttttcccg ttcttgaata ccatgttttc ccaccaactt tttcttcatg ttttccctct
     9661 cctttttcat atcattttaa atataatatt attacgttcc cgcgttggct tttttagcaa
     9721 ttttagctta tttccatgcc atacgtcttt atattttgta ttatatactt gtatttctca
     9781 ttttacaaag ctataataat tatacttatc atggcatttt agttatagta aattccagtt
     9841 ttttacattc catggatttt atttgtaata tattaaagaa gcaataagaa agggaccgat
     9901 cttatatgac aacacgacaa ttttattacc ttaccgttat agcggatctt ggaaatcttt
     9961 ccgctgcagc ccagaaactg cagatctccc agcctgcctt aagcaaattt cttacggaat
    10021 atgaagcttc ccttggcttc cagatctttc tccgttatca ccgacatctg gcacctacct
    10081 ctgtaggccg ctgcgtgatc gaatacgcac ataagatcgt cagtgagcag aacagacttt
    10141 cccagtcctt aagaacgatc atcaacaggg atcatgcctg tatccgcctt ggaactactc
    10201 ccaaccgtgg ttctgtgatc tacagccgga tctatcagac gtttatggat cgttacccgg
    10261 atatttccct gtctctcaca gaacgatact ccaatgaaca gacagaagcg gttctgaaag
    10321 gccaagtgga tcttaccatc ggctccggaa aatcttctga taaagtaacc gatatccccg
    10381 ttgcgcatga ggaactcctg gttgcccttc ctgcggctca tcctctggca tcttccgata
    10441 aggtccgtct ttctgacctg aaagatacgc cgttcgtact ccaggggcca cgtcacaata
    10501 tccgcattct tgcagaagag ctttttaaag aagccggatt tgaacctgta attacctttg
    10561 agtccgataa tgtgatcatt gttgactcca tgctccacca tgctgccgga gcaggctttg
    10621 tatcaaaaaa atacgccgtt ccctgtaacg aactggtttt tctcccctta gatccgcctg
    10681 tataccaaga aacccatatc cgctttcctt tgggacataa actgacagaa ccggaacagt
    10741 accttgcgtt tcttctgatc cgtgatatcc agtccaatcc agaacagcgt tttgagatca
    10801 ttccttcaaa agaagtaaat gatctgctcc aggctgctga tcctgatatg agcagtaaaa
    10861 acgatcctgc ttccatacag ggcggacatg cagctgcatc ctctgtacag gggtattctg
    10921 agttaaactt aaatccccag cttcttcgtt atgtgatcgc cattgtagat gaaaaaagcc
    10981 tgaccaaagc cgctgaaaag ttttatctga cccagcctac tctttcccgt tatcttcacg
    11041 gcatggaaaa aatgctgggt gccagattat ttaccagaga acacaacaaa ttacttccaa
    11101 ccaatgccgg aaaggtattt gtaaactttg cccgcaacat cctacagatc gaggaagaaa
    11161 tgtcagaaca catcaaaacc tatcagcagg gacatgacgg tggtatctat ctgcaatgtg
    11221 accccagtct gctacacatc atccagacac aggtcattcc cagttttaag aaacagcacc
    11281 cggatatcca gctgaatgtg agcaccaaca gccgtgagca gatccaggag tcacttttaa
    11341 atgcatctgc tgattatggg gtctattttt cctgcgaaga agaacatcct attttagaca
    11401 gccgcattct ggatgaagat gaacttgtgt attgttccaa acatgatctc ctttctgatc
    11461 ccctggagca tactaaaatt atgacggttc caaaaggaaa tcctctttac gctgaacaga
    11521 aacgcctgtt tcagcagtta tatctgactt tccctgcaac ttcctgtgaa gcagctccat
    11581 ccattctttt ttttctggaa aaaaatggaa ttggaaacac catattgcct tctaaaacaa
    11641 tggcaaataa cccggatgac cgccattttc ttacaccggc cccgggatat tttctcatca
    11701 tggcccatca tcccatccgc accatgcctc cggtgaccag ggatttagaa actctgcttt
    11761 atgatacatt tgaaacatat tttgagcatt tcgcggagtt attttcataa atatggaaaa
    11821 aatgtagatg catggcaatt aaaaaggcag ctgcatatcc ctggacttta tcgcttacct
    11881 gtccaagcaa cttgcacaca ggaaggtttg ctacctttcc aaggatatcc cagagagcca
    11941 catcaatgcc gctgatggca cccatgacca ttccctgacg gccatttgcc cagttgttcc
    12001 acatcatctt ctgatggaga aattcgatct ttgtgggatc ttcatctaaa agtaatgatt
    12061 ttaactgttt ttctactaca aattccattg catccatgga acttccaaag gttgctgctt
    12121 ctccgatccc tgtaagttct gtatctgttt caatgaagat aagaaatgat caccggcatc
    12181 agcagtgcca ggatggcatc cttaaatgcc tgaacttttt cctctgtggc agctctttcg
    12241 cttggctcaa atccctgttt tttacataag aagtgggcaa ccacacataa caccagacag
    12301 atgaaaattc ccggaaggaa acctgccata aacagatcac tgatagaagt tcctgtcgct
    12361 acaccgtact ggatgagacc gatacttgtg ggaatgataa caccctggtc tgcagtgctg
    12421 taaacagttc accaaaactc atggacactg catcgatccc tagctctcca agagagtcaa
    12481 cacagacgga actttccagc attctcatcc ggatgccctt tgcgtcttcc ggatgaacga
    12541 tgggtctgct ggaattggtc atgcttctaa aaccaagatc aaaggttcca taggtctcaa
    12601 ttccctattt ttccagtcct ttaaaaattt cctgtcctgt ttctccgtcc agtaccttaa
    12661 aagcttcttc tctggagtca aacagatata gaaagttaaa tacgtccata tccgggtcaa
    12721 atccgcttaa agttgctgca actgcaactg tgatgtcaat ggttccaaat ggaagagatc
    12781 accatgactt ttgaagccag actgctcatg gaaccctata ttattgaaaa ttacagtaaa
    12841 tatattgata tggataaact tcttgactta gaagaacaga caaaaaagat cttaagcagt
    12901 gatatcttag agcagaagga tgctgttgtc ttctgcaata ttgatgatgc cttacaccgc
    12961 accattgccg atgcctgtaa aaacaaatac ctgaacatga acttatccca gatctatgac
    13021 cagaacatcc gtatccgcat cttaggcgaa caaccgaata attcttttaa aggctgttgt
    13081 aaaacagata aataattatc cattctgcaa cagccctttt atctttatat atgctaaacc
    13141 agataattat ttggcaggcg cacgcttctc ctgcatctct caggtttccc ttgtctgtac
    13201 cagcggcgtg tggatcgtgc gaaattttcc acctcaaata atcatctggg taaagcctta
    13261 tttattttct tttttcaaat actccgtata gggcacatcc ggatccagga tcagtccccc
    13321 atgcatagtc ggggctttgg ccggtttcat ataatcacca tattcggcgg tgagtctgcc
    13381 gtcatagcct acaggaacag gtatcttacg gttttcaaag ggcatgtaaa cagcttcttt
    13441 gaaccactcc cgcttccagg tgtttttttc cagatagcgg ataaatacag tgtcgcagac
    13501 atattccgta tctttgcctt tgaagcgggt acacagtttt tcataaccct tatatgcctt
    13561 gaccatggga acatggagaa gcattccaat gccatggaga agttttccaa aaaccgttgc
    13621 attttcataa taatcaaagc ggtaccaggt ataaagcacg gtccaataaa actggatctt
    13681 tttggcccag ttttttcttt ctttttccgt tttcggcata ccgtccaatg gaaaaatatc
    13741 aataaaaatg ccgcagttat atcccgcctt tttatcttct tcattgcagc cgcaggtctt
    13801 gctgtttcgg atctgagcgt ggccgcgaag ataatttttc tctgtataca cagtctgaag
    13861 caccagatct tctttcagcc atttcggggc aatctgcaca aatttatcat aatcttcccg
    13921 cagcatgacc aggtcaatat catcatccca tggaatatat ccttcatggc ggatagcacc
    13981 gatcaaggtt ccgctgtcag caaaatattt aagcccattg gctttgcaga tgcgctccac
    14041 ttcatctaac atttccagct gcaccgccca tacacgcttc gttttttcat ttactgtgta
    14101 tccgcagcgt acttcctctt tataaaattc cggcttttct ctcagcatat tcaaatgatc
    14161 cttccaaact gtttttatct cacattacat ggcattcctg tgatttttta tggctgtccc
    14221 gtcagccgcc tgtatatttt ccctaaatac tgatacagga acggatcacg cattaccaga
    14281 agcaccagga aatacaccat tgcacctgca ccgatctgaa taaaagtggt aataggtccc
    14341 aggccccgga cctgttccaa aaactggccc gtccacacca cagctcccag cataataaca
    14401 gccgccacaa gacgcctcca gccataacgc accagcattt tcagctttat aaaatctctt
    14461 cccagccaca gataaaggga ggtgataaaa cattctgctg ctactgacgc tgctgctgct
    14521 cccaacgctg caaaatgagg gatcagaagg aaatttaaac aggcattgac catagctcct
    14581 gctacgatca ctttgccgct ctgggccctt tttccaccag gcgttaaaat ctggctgccg
    14641 atgcagtcac ttaaacctac caccagcacc agaggactgc atacatacat taaaaatgtc
    14701 accggcagat acccgtttcc caaaaaccag ggaataaact gggctgcgat ccccgccaga
    14761 cccagcataa taggcattgc cattaaaagg acaaaatcca gtgagtcctg gaaacgctta
    14821 tggatctctt ccatccgtcc agttccaaat aaataggaca tacgggcgga cataactgca
    14881 ttaaaagaca tgatcagtat tttcgccata ttcacaaaac cggtggcata ttcataataa
    14941 ccgttctgtg acttattttc cccggaaaac cagccaagca tagtcttatc caaaatggta
    15001 tagacggaag tggcgatggt aggtataaag taaaccagag tctcttttaa atggcgcaaa
    15061 ggacgcagtt cttttaaaac cggcttttcc acctgcccct ttaaatatcc ccacatggaa
    15121 acatttccca gaagtccggt tgccgcagtc aatgccacat acagcagaag atcttctttt
    15181 tcccggataa ataaaaacag catggcaatg cccacaagct tcactgccgt atttcgcaaa
    15241 acgatcaggg aatactgctc cagcccccca taaaaccagg aaatatcaaa agcaactgcc
    15301 agcagtgtca tcgtcaatac cacataataa ggcctgtatt ccctggcaaa gccgatcaca
    15361 aacagccaga taataagaca tgctgcggtc gttgccatac aaagcacctc gatctcccag
    15421 aaaagccggc tgcgtatctt tatctcatct ctgtgtcttg cgatctccct ctgcccataa
    15481 gaagccgttc ccagagccgc cagtatggca aaatactgga ctacggaatt ggtataactc
    15541 tgtactcccg ttccatctgc ccccagaacg cgggaaatat acggggtcgt gataaatggg
    15601 gtgatcagcg tcatcacctg gtaaattaaa ttgtatatat agtttttccg aacactgggt
    15661 gcttttgcca taagctcttc tctatactcc atttatattt atgttctctt aaaatctttt
    15721 ttgtctcttg gctcattata cccatggaaa cctttttaag caaggcaaaa atccgcaaca
    15781 atgccagtac cggcattatt gcggattttc cagataaatc tgtctgcaat gttctaatct
    15841 tccatcatat gatcgtattt ctctcttgtt tcttcccgtt ccagattgag cacataagaa
    15901 agacctttta atttcgtatt caggatcagt tttttcggaa attcgcaagc ctctaacatt
    15961 tcctttgccc gatccaggtt tcctatatcg taaaatatgt gagcatcact gtccacgatc
    16021 accggcacct tgtatttttt acagaaagac agcataaccg gaagattttt gtctgcattc
    16081 agtctgccgg tacggggaga aaaagagctg ttgtttactt ccagcgccac tttttctttt
    16141 gctgccgctt ttaccagttt ttcataatcc agaggaaaac ggtcatcatc tggatgtccg
    16201 atgatcttca catatggatt tttcatagct ccgatcaatg cattggtatt ttcctctctg
    16261 gttcctgctt ttacacaggg aacatgaaga ctggcgatca catagtccat tttctttaaa
    16321 acagcttctt ccgcatccag ttttccttcc agatctatga tattggcttc cataccggta
    16381 aaaatacgga ctcccagtat tttctctttg atcgccttaa aattagtaaa ataaataggc
    16441 tgcgcagatc ccggcatggc actgtagtga tctgatgttc ccatggcttt taatcctttg
    16501 gccgctgcct cttctatatt ttcttttaat gtgctaaatg catgaccact ggccactgta
    16561 tgtgtgtgaa ggtcaaataa cagcttcatt ttttattcct cttttcttat gatgcctggt
    16621 aaaccagctt tacctgttct cccggctcat actgttctct gcttcctgcg tataccgtaa
    16681 atgtatcttc cccgcatttt aaggaataat ggatctccct gccgctgtac tgcctgttgg
    16741 ttacagttgc cggaatacct tttccttctt ttacaatgtg aaactgctcc ggacgtacag
    16801 gaaacagacc atggttttta aaagcctgtg ctacttccgg tccatcatat ataagggact
    16861 gatccattac tttaaaatag gaatcattcc attgtcctct gacaaaatta gatttgctta
    16921 caaaggacgc tgcaaattca gtctgtggat gcagatagat ctcctcaggt gttcctacct
    16981 gttctatctt tccatttttc ataatgagga tacgatctgc catggcaaga gcctcgctct
    17041 ggtcatgggt cacataaatg atggtcgccc cggtcatacg gtgaacatcc tggatcaccc
    17101 ggcgcatact gatctttaat tctgcatcca aagcacttaa aggttcatcc attaaaagaa
    17161 tggatggctt tcccacaatg gctcttgcaa gagagactct ctgcctttgt ccaccggaaa
    17221 gttctcccgg ataccggtct tcataaggct ttaatcccat attttcaatg gctgcatcta
    17281 cggcttcttt tttctgtgcc tctgtcaggt gtttctgctt tggactcttc attggaaatt
    17341 ctacatgctg gcgcactgtc atattgggcc acaaagcaaa ggactgaaat accattccca
    17401 ggtcgcgctg ctctaccgga accatttttc catttccgga atagatcaca tcatccatcc
    17461 gtatctttcc ctctgtaggt tttgcaaatc cgccgatcgc ccgcaaaaga gtggtctttc
    17521 cacagccgga aggtcctaaa atagccagaa actccccatt tcgtatttcc agatcaatgt
    17581 cattaagcgc cagtgtttgc ccgaacctct gggttatatg ctctaaataa agactcatgt
    17641 tcttttcctc tcttcttttt ttctgccggt tttaaagcca ccagacagta acctgtaaga
    17701 accattacaa cgatcacagc tgacatagct gccgacagat tgtaatctcc agactgctgg
    17761 aagttaaaaa tagtaagtcc tatggtcttt gttcctgcac ttgcaagcat agatgacaaa
    17821 gtaagttctg taagtgatga tacaaagatc aaaaaggaac cggataaaat aggacgtacc
    17881 agcataggaa gcaatacctg ctgccagaga gctcttttgc ctctgccaca ggcacgtact
    17941 gcttcttcca gctccgggtt cacagacata agagctgtgg tgctctcttt gatctgaagg
    18001 atcagatatc tggttatata agcgatcacc aggatccata tggttccgta tactcccgga
    18061 cggatcccgg gaagcggctc tacccagtgg aagatcattg ccagagaaag aacgatacct
    18121 ggaatggcat aggtcagaga agcacatttt tccatggtca cagccccttt actgtttctt
    18181 cttactttca tataggctgc tgcagttccc acaacaatac agactccaca ggtcacaagt
    18241 gccagaagga tactgtttct gacagcggca agaacacctc tgtttgtaaa tacaaaagca
    18301 aaatttttaa gggacagatt atccaacgtc attttcaggc catatgtttt taaaaatgcg
    18361 ctttttatca ttaccagaat gggaaccaga tttaatacac ctaatgttcc caacagcccc
    18421 cattctaata gcctccgttt tggtccttcc agctcaatac gtaccgaata atcttcttta
    18481 atgctttctg attttccatt ttttcccact aatgctgttt ccatcaatgt accgcctact
    18541 gcgatcacag ataaaagaac agaaagagca gctgccagag ggaaagcgtc tggtccaaat
    18601 ccaattgctt tttcataaat ataggtgctg agcaccggga tgccagaaga gatacctaaa
    18661 aatgccggca cagagaagtt atcaatggct gataaaaacg ccagaagccc gcctgcaatg
    18721 atcgcaggaa gcacctgcac cagatccaca tggaccagtg tataagaaag accaaatccg
    18781 caggctctgg aagcccattc caggtctctt ggaatacgcc gaagcatatg gatcacgctc
    18841 atgtatacaa cagggatgtt gcagataccg atcaccagta aaataccgcc aatggaataa
    18901 atattcaccg gaccgatccc cactgcccca aggatctggt ttacgatccc ttttttactt
    18961 aaaaatccgc tccaggaaag agtaataata taagagggga taatgaaagg aagcagcacc
    19021 agtaattcta aaagcctttt tctttttata ttggtataag ctgctgccag agccagtccg
    19081 cttcctgcca cgatggcgat catggtagag gaagcagcga tcaggatcgt attaaagatc
    19141 gcttctctgg tccgtttttc ctgtaacagg acctgataat tagaaagtcc ataaccatcg
    19201 cttcctttca ggctcataaa cagcaaacgg acgatcggcg ctataaatac caggaagact
    19261 cccaggtaaa acagctgcag acctgtactg cctaagactt ttttatgttt cataacaatg
    19321 ctgccccttt ctgcctgtat acctgctgtt actggaaaat ctcagaaaat ttctctttat
    19381 ctgcgtcacg gctgctgtaa agttcttttg tatcagcact gatggtcttg atctgatcaa
    19441 tggattttaa tccttctggt gctgctacac cctcttttac aggtgtataa ccaatagaag
    19501 ctgccagttc ctgaccttca tcagaaagaa cgaaatctac aaatgcttct gctgcatcct
    19561 ggttcctgga gtcttttaac acaccaattg gttcggtaat ggctggtgaa ccttcttccg
    19621 gataaacaaa ctctaccggg gcaccatcat ttttagaacg gtttgccatg taatcgacca
    19681 ggataccgca ggacttttct cctgccacta ctgccttctg tattgctccg tttcctttat
    19741 ctactgtaat tccgttatcc tttaatccct ggaagaaatc ccagcctaat gtttcattgc
    19801 ggctgagtac ccccagattg taagctgccg caccggaata caacgggctt ggcatgatca
    19861 ctgcatcttt aaatgcctct cctgtcagat ctgcaaatcc ctttggcgca tcttttacca
    19921 gatctgtgtt gtatgcaata cctgttgtga tcacctttgt tcctgtatac atatggtctt
    19981 tatcaatgta ttcttccgga atgcctttca gttccggtga ctcataaggt aaaagcatat
    20041 cctgttcttt taaagtttca aatgtaacac tgtctgctac taaaagcaca tctgcctgaa
    20101 cagcacctgc tgtctgctct gccaaaacct tgctgactac ttcttctgtt ccggaacgga
    20161 atacatctac tgttacatcc gggcactctt tgttaaagcc atcgatcagc ttctgtgcat
    20221 cttcctccgg ctgggaagta tataatgtga tctttccgga aacatctttc ttttctgcct
    20281 ctgtggaagc ttctgtcgtc tctgcctttg cctgtgtagc cggttctgct gtggtcttgg
    20341 caggtgctgc tgttgtttct gttttatcag tctgtccgct gcatcctgca agtcccataa
    20401 ctgctgctgt tgttaagatt aatgccattt tcttattcat ttttaaagtc ctccgtttgt
    20461 ttttaaaaaa gctgtatttt ctgtaaatgt atgaaaatcc ctgctgtcat catagccttc
    20521 tgttaccaga gggagtccat atgctaaaag ttgtggaagc atttctttaa atgaaaaatc
    20581 accgttttct aaaggagtat ggtaacattt tcctttccgg ttgctgatat gtatcttgga
    20641 gatccgtttc atctggtcta atatgtgaaa tggttcttct tcgctgtcac aatgtgctat
    20701 atccagagta taaaaaaact gttcttttct gcttcctgct gcttcttcca tactctgtgc
    20761 atctgttaca aattcttttg ggatcttttc catgatctct aaagaaacat ctactccgat
    20821 ccggtcagaa agccttccga tctgttctaa cgagtcctga agaattttca tgctctcttc
    20881 tcttcgtccc ggaagtgtca tatgtcccgg atgaactgtc agttccattg cttctaactc
    20941 tgctgccagc ctcatagatg cttccacctg ttttatggac atttcccgga tcccctgatt
    21001 catggaagcc agatttaaat cccagctgta g
//
LOCUS       DBMDUA010000023        20717 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1593_length_20717_cov_3.141977, whole genome shotgun sequence.
ACCESSION   DBMDUA010000023 DBMDUA010000000
VERSION     DBMDUA010000023.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 20717)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20717)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20717
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1593_length_20717_cov_3.141977"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            35..952
                     /locus_tag="ACZPCL_03325"
     CDS             35..952
                     /locus_tag="ACZPCL_03325"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1161551.1"
                     /translation="MTLSDYIIFTTVSELKNMSLASDKLYLTRSAISHAISRIEREIG
                     FPLFIKNTHGIELTDNGAALLPLAYAVIQNHQHFNEKIAAINGLASGNVRLGTCSSIC
                     INWIPELVNNFRHHYPDIQIHIFAGINNAQIVKKLEQNEIDIGISSYYSCDTISSSAI
                     SSTVIYEDEMVCVANSDFHTSTPGIITAEELQNSAFIISDQDYGVESRSVLEKLHLNA
                     NSTITATDDAGLVAMASAGLGFCILGRLVLKGTTSPVNVYSFHPRQFRHIALLQKKNV
                     ALSPAAEIFQKHIISYASSYPNPAIPFNF"
     gene            1077..1880
                     /locus_tag="ACZPCL_03330"
     CDS             1077..1880
                     /locus_tag="ACZPCL_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR1161552.1"
                     /translation="MQIKQENDGHAIIDQLTGEEVLTCAINIGEQLLMSGAEISRVED
                     TIRRICAAYDIRQSHIFSIASCIIVTLETKDHKWITQTRRILSYGTDMWKLDRLNNLS
                     RLICSTKPPLERINREYALILNGPVYPPVVQCLIYAMTAGAFAIFFGGNLLDGFSAMF
                     VGALIRITLYAFTAIKMKAIFSNILCSMISGMVCILTHYIGLGHHVEMIMIGNIMLLI
                     PGVLMTNSFRDFISGDMISGLLHFSEAIITAICIAAGFIFSKILLGGIL"
     gene            1877..2329
                     /locus_tag="ACZPCL_03335"
     CDS             1877..2329
                     /locus_tag="ACZPCL_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR1161553.1"
                     /translation="MNQELISVISAFFGSLGFSIIYNIRGLRIWIPAIGGAVFWAVYL
                     IFLHFINNEFLGFFFVAILITIYSEIWARILKTPSTVILMPTVIPLIPGGSLYYAMDA
                     ALRHDMPQFFLKAQAAVGLAVALAAGIMAVTSMQHLISALIKMIKPTP"
     gene            complement(2366..3544)
                     /locus_tag="ACZPCL_03340"
     CDS             complement(2366..3544)
                     /locus_tag="ACZPCL_03340"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR1161554.1"
                     /translation="MWNECKDLQEYIVGLRRDLHQIPELGLHLPETAAYVTAELDKIG
                     IPYICSKKDSGIIATIKGGKPGKTICLRADMDALPIKEETGLPFASRHEGFMHACGHD
                     THTAMLLGAGKVLWAHKDEIAGTVRLMFQTSEEQSRGAEVMIENGGVEGADAVFGTHI
                     GTIIDKAIPTGTVVVTPGCCMAAFDKFVIRVNGRGCHGSTPEKGIDPVNIAAHIVIAL
                     QAITTRELNATKPLVLTIGKIQGGSQYNVIPNEVVIEGTIRTLEEEVRQFAAKRIGEI
                     AQATAAVFGGNVDYEMIWGAPPVINDDKMAALAAKAAKTVAGEDHVITEVKAPNMGGE
                     DFAYYLEKLPGAFMFLSSSNPAKGTDVSHHNPKFDVDEDVLWEGSAVFVAIVEEFLNK
                     "
     gene            complement(3547..4275)
                     /locus_tag="ACZPCL_03345"
     CDS             complement(3547..4275)
                     /locus_tag="ACZPCL_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161555.1"
                     /translation="MKDYMAEWKRNSIRIGAPTCIMAAFTAFIPVLYLCSRYGCWPKL
                     ETVLAAWALTALSFGAFYIVEPISYYAALGMSGTYLGFLSGNIGNMRVPCAALALDVT
                     DSKSGTIQAEVVSTMAICGSIITNLIATTGAVLVGSAVVAVLPAFLNSALKNYAAAAI
                     FGGTFGNFAVKYPKVAVFGLLIPTVLKLFVPVPAWLVIVCAVFGTLGMARLFYVSDKK
                     KDGNADDTAKAEKINETPVGEEIA"
     gene            complement(4292..4999)
                     /locus_tag="ACZPCL_03350"
     CDS             complement(4292..4999)
                     /locus_tag="ACZPCL_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007036304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HIR1161556.1"
                     /translation="MDYLAIANAPIFWALCGITVVISLAQALLFMRQAKRAAVEVNLE
                     QGLANKAFKIGLISAIGPACGVFIVMVGLMASIGGPMAWLRLSIIGAAATELSAATMG
                     AQAAGVEFGGNGYTLTVMAVSWFAMALNGAGWLLVSGTVTPALEKLRGKLSGGDAKWL
                     AVLSGACSLGIFGYLNANEIKKGLGSTIACLAGALSMVAIMKLIVPKHPKLAEYSLGI
                     AMVIGMFFAVMHDLAVA"
     gene            5272..6894
                     /locus_tag="ACZPCL_03355"
     CDS             5272..6894
                     /locus_tag="ACZPCL_03355"
                     /inference="COORDINATES: protein motif:HMM:NF037653.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HIR1161557.1"
                     /translation="MTISDFLKLPNFTDLRLLAGKYGLLSEVTNVTVVDTPDGTNWLN
                     GGELVITTAYMLHEEKDLLEFLRTLSSKKAAGVGIKENRYLTEIPESARKLADELHLP
                     LISVPEAYPFVDIINPVLTQIINQQSFLLTQANMIHKEFLSLAINNNSVPEILMTLRR
                     FIGIPCAFMDTHFKNIFFSDEDSPLMRQLQDMDMENISSEFLNQYDNYAVANKNESFG
                     YLLFEKGRLDTGNESSAQIALEYASIVLILHSQVRIANQQMAEKYKASFLEDLLLNNV
                     KADIEIHNRARLYGWDFTNGGLAAVVDINNIKKYFIDRLDSNTNRMLEEATELIFRNS
                     IHEMHQTFPQAKYFRQSDLIVFIISVPKDSRELLADQLEQTFKRLQSQLSHVSPFTIT
                     LGIGQYYENIREISKSYSEARVAINLGYSLQWFDRILFYNRLGLYRLLAPVMNSPESE
                     ELCLQYIKPLEDYDKKYHGELLSTLQEILQCGWNLKESAEKLYIHYNSIKYRYSKICK
                     VLNLDLSDHDNRSLVEIAMKLYRLKHYKKENQ"
     gene            complement(6955..7392)
                     /locus_tag="ACZPCL_03360"
     CDS             complement(6955..7392)
                     /locus_tag="ACZPCL_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR1161558.1"
                     /translation="MAELYIKDGIGIFDNKAVNADGKAAGDDLVFSIYGEHSAYTATL
                     AERMLGGGAVKWYRDGTVYNPTPTGYTQIGPGSQRYEEGSGAVPLSSDEEAGSADIEE
                     RKDEEMAIQTLPKTGRSKDYTMFAWLFSGLILLAMVILKRKVK"
     gene            complement(7446..7931)
                     /locus_tag="ACZPCL_03365"
     CDS             complement(7446..7931)
                     /locus_tag="ACZPCL_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161559.1"
                     /translation="MTGGAIQNNTGHRGAAVNIYSDDRDKRAIFTLEDDGLITRNTGI
                     SANGNEAGGAVFVEDNAAFHMIGGTVSANKASKGGGICIVDSALQRGTAEYGTEFLMD
                     GGVISGNRAGTGGGIYSFSNGSFLRSGKIINNTATYSGGGLYSEGKALMVTALPIYII
                     P"
     gene            complement(7968..9245)
                     /locus_tag="ACZPCL_03370"
     CDS             complement(7968..9245)
                     /locus_tag="ACZPCL_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HIR1161560.1"
                     /translation="MDDSKDDPKDDSKDDQNGPKDDQDDPDNSDASGNDQEDQKPDQD
                     ETPDENTDSADSSDNSGKDDNGNSGNKDSLSDKNISPNPEKNGKNKEMSLLTVDYEDV
                     KMEIDGITYTFDLDPDDHTATLKDISNPAEASEIEIPEQVTYDEDEAEDNEYTVTILR
                     WGPFAGSRQNVSALMIPDTVEKCDVYFSRFSNLTEITIPGSVKKFGGNFQNMDKLETI
                     TVEEGVQEISGNSMVSNCSSLETIDLPESLKYITGPAAFASAEKLKNISLPDGVEIKK
                     ASGLFSDDISLTSITLPDSVASIPQSAFSGCSALEEVTAKGQITEIGQQAFYKDTALA
                     KIPDSVTSIADHAFEYTSIEHLILGSGLESIKNDAFSNIGTLETVTIRNSSDAVDISK
                     AGFSGNAVITYTEKSISDSVGSAISDEAGAPTL"
     gene            9955..10347
                     /locus_tag="ACZPCL_03375"
     CDS             9955..10347
                     /locus_tag="ACZPCL_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161561.1"
                     /translation="MKSVYKIPEKIKGLSDKRKRRSIPLFNIIMPSLLFLMLQYESFH
                     TVFSAPESMGKRLKNCIHGKIPKIDAVRELLTQIDPDEIREIHEQTIDIIKRNRVFRE
                     GTIGGICCSRNRWSGIVQQHKKVLFGLP"
     gene            10401..10532
                     /locus_tag="ACZPCL_03380"
     CDS             10401..10532
                     /locus_tag="ACZPCL_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161562.1"
                     /translation="MTVGKVPHVILGQEMLKPKDGAEKDEGELTGGKRGRLPVEKRI"
     gene            11147..11611
                     /locus_tag="ACZPCL_03385"
     CDS             11147..11611
                     /locus_tag="ACZPCL_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HIR1161563.1"
                     /translation="MTEKKAKRWAGELLLVWMVLYNWYRFDYYNEKSILGTVLHGCGK
                     LLHIPMKRAYGFYEKLCKRYDEKPTRFVCDTVFIRYLEKNTWERSWFDAVVYLPFENR
                     IIPAPTGFDGRLRTEYWDYMKPSKAPTMHGGLILEPDVSYVEYLKEKMPRQK"
     gene            complement(11632..12993)
                     /locus_tag="ACZPCL_03390"
     CDS             complement(11632..12993)
                     /locus_tag="ACZPCL_03390"
                     /inference="COORDINATES: protein motif:HMM:NF045042.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase family protein"
                     /protein_id="HIR1161564.1"
                     /translation="MEIQIEEALNLNLGSPALQYKVMPAKLGNGRKGFVAFYSACYDV
                     DVHYSMFVVPSDTLHGIAFDEHGEVLWKKELGIWPSAALYCFTLIDMDQDGIDEIYIV
                     NNSDPIHPLWVEAYVLERMDVLTGNVTGQWPWPNYGGIQKSMWYFRNHVFGGYDHGQP
                     VLVTAQGTYADMYFQAWNPDMTSKWTLSIGENAPGARGSHSFPVVDINNDGCDEVLWG
                     ERCLSLHDGSELFCIEKDTWNGHSDMCQPVFDYDSNRWLIYINRESNEQIGPRIGMYD
                     TSGQPIWTHVDWGHIHKGWVGRIGKEGRLLANGMRITGQSKDAFGRYYTGITEFVYDA
                     MTGAPISLPYSLFDTAPIDINGDGLHEILRNVAAGDTELIDRDGNRICHLGGKVAMNT
                     KILNFPGEQVMLYYPDGTIRIFRDKNAVDSETAKKRYQLPFYLRNQYARNIENNICML
                     GGI"
     gene            complement(13021..14424)
                     /locus_tag="ACZPCL_03395"
     CDS             complement(13021..14424)
                     /locus_tag="ACZPCL_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024808.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1161565.1"
                     /translation="MRKRKATRYLGFTLTATALCCLSACGSSSSSTPAKDTTATKEET
                     SSSAQNSTAETASEKEAKTADEKITLTFWDENAGEKRTPYYEELIKRFNDSQDRIVVT
                     YEGIPSSSAKEKYDVAIATNATPDIGHCNAAWGSNWLAQECLVDLDSYFDAWDEKGDI
                     NENYLRLNRELDPENRLFMITDTVSTPLMWVRTDLLKEKGLDVPKTWDDFFTTAEKMT
                     DASKGVYGFSFRGDSSSCVELQHAMYAYSGIEDYFDENGKCTINAPEHVEFVTRYAAL
                     YNKYTAESDITNGYKEMVAAFDGGSAAMIFHNTGSAAEHASALQPDQYVAVSFPESLT
                     GTRSYTAGSQGGFCIFNTCEHPDEAWEFIKFMLSEDSNSYWNKSIGQIPMNNKVLKED
                     WVSKSQSTMEALNTLTASNCHLVSQPKYLPDYSTIMNTNNPANFQAMLLGEMSVQSFL
                     DNWAEQMNEAYAEYQAQ"
     gene            complement(14509..15348)
                     /locus_tag="ACZPCL_03400"
     CDS             complement(14509..15348)
                     /locus_tag="ACZPCL_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008717034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1161566.1"
                     /translation="MKTISKKRRELTCIYLILLAAVIWTLFPFYWTFITSLKSSAEIA
                     AKPITYWPVHVITENYSNAWKTGGFSRYFLNSLKVSLISTVFTVLFATMDGYALARYK
                     YKGKNAFLLILLCTQFFPTSMLIIPLFKIYNMTHLINNHMSLILTYTAFHIPFNAALM
                     RSFISGIPATLEEAARVDGCNRLQAVTHILMPLLLPGIAAVSSYSFISSWNEYLYALM
                     FMSSDSKYTIPVGLSMTIGEYSINYGQLCAGSVIALLPILIMFAYVQKYMVSGLSSGA
                     VKG"
     gene            complement(15356..16258)
                     /locus_tag="ACZPCL_03405"
     CDS             complement(15356..16258)
                     /locus_tag="ACZPCL_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1161567.1"
                     /translation="MKTYFPNHKRPVTLIPYMLMLPTILLLCWLLVYPVFNVFYTSFW
                     QEMLSRPKANAFIGLKNYHKILSDPVFFEALGNSFRWVIVEVGLQLVLGLGLSLILKR
                     KFRGCGIFRSILFLPWALSGVLVSMLWSMMYYENVGVLNDLFQRLGIIQKAIAWTGNK
                     HTAFWSLIIAELWRGIPFFSIMLLASLQSVPDEMYEACRVDGGNALQSFFYITIPHIK
                     ETLVLTTLLRVIWEFNSVDLILNLTGGGPVHKTTTLSVYLANTARKDGNFGYGSALAV
                     ISFLILLIFAATYLKLSKFGNEEE"
     gene            16425..18146
                     /locus_tag="ACZPCL_03410"
     CDS             16425..18146
                     /locus_tag="ACZPCL_03410"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1161568.1"
                     /translation="MKKRFKEMSLRSIIMILCSSFVSLIIVTSATFYITGISHINNLV
                     KQNAANLTIQAAENMDKRFRKVKDSFFSSIMESYYFLKMGQNIMENRQPISAKHYAEL
                     ARKIQDYVNQNRQDVSVVILMLSNNSIVLTASGEEEFYRCYQPEYDAFYRKYEDGKLN
                     WIYSGDAAGFMGNSGEIPHLGMIQMLGSRDSDIHGFIYIGMSDKGIARELGSYHITNS
                     NSLALMKENTFFLTKDLSAGTADIDLKENYVVSEHLKEVPLDVVAIIPKNELFIDRNN
                     FQILVFGESVVSVLFCFLLFMVITKVLSAPVEELVRQLDSVENLTQLESARAVGGKEF
                     RKITEAINHLYQRIQILITSLKEENQYRKDAELKILYAQINPHFLYNTLDSIGQLCEM
                     GESESACQMVYELADFYRIGVSKGADFITLEEEIRHVTAYLSILQTRFEDFHFQIEVP
                     EELAGTYVPKIILQPLAENAVYHGIHSGNGDGNIRITVKREGEDLFLEISDNGNGIEE
                     ELLGKIRRSLQLDEVKAEQDAKIYGLKNVQQRIVLQYGAQYGISIFSMLEQGTRVLIK
                     LPYLTQI"
     gene            18162..>18949
                     /locus_tag="ACZPCL_03415"
     CDS             18162..>18949
                     /locus_tag="ACZPCL_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1161569.1"
                     /translation="MTKILFVDDDAIARRNIRTKIDWKSYNWELIYTAKDAVDALDFM
                     KQNQPDIILSDIKMPIMDGIQMAMVARNYYPDIKYIFISGYKEFEYAKQALKLNAVDY
                     LNKPLETKQLVQVLREAERLSKEAKRNNDILRDKYPFLQRHYLSQLMYQHFREIDAAV
                     FKAFDINLSDGYGIAGYLQICTDENSAGTDRAEKLGRYLSSIYEGSFFISMGVNQIFL
                     IYTCSNVENEASFKVRIEKMEWQAQQYMKKGETLVFHYGTVIRNL"
     assembly_gap    18950..18959
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18960..19336
                     /locus_tag="ACZPCL_03420"
     CDS             <18960..19336
                     /locus_tag="ACZPCL_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1161570.1"
                     /translation="NELYVSYESILKKIDMDTNDLLAKVKSYLNQNYFVEDLSLTQIA
                     EYFHVNHCYLTSIFKEKYGINLYDYLIQTRMKKAGELVCSTSKKVYEIAEEVGYKNSR
                     YFSVVFKKYFGCTVLEYRKQHT"
     gene            complement(19438..19704)
                     /locus_tag="ACZPCL_03425"
     CDS             complement(19438..19704)
                     /locus_tag="ACZPCL_03425"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR1161571.1"
                     /translation="MIHVIYETQNPYTLFPSTYNWLGENRSHGCICFTTADSRWICEH
                     CPLKTTVNVYESPVPSPFDHPAIKTLISMDQYYDPTDMNLPENQ"
     gene            19773..20444
                     /locus_tag="ACZPCL_03430"
     CDS             19773..20444
                     /locus_tag="ACZPCL_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161572.1"
                     /translation="MTEQSFTAEQLEQAARAILIDHKYSISDIKDQYKQQIYEALIKL
                     SPAFALSYIPESERTPYALKYFSQNQIIEDLCDDAIQRNGDCLAFILNRYKTPDRCLT
                     AIGNAGYEILDYIPDKYKTDESFMLEEIARDEYSPLKLDQSIWDKPKHFWIQAINKNP
                     LSIVMVPKQYKDIHMSTIAVAMNAEALAYIPKDGWTKERSNPMNNFQVSSILIRDFAF
                     STVYL"
BASE COUNT         5974 a         4408 c         4455 g         5870 t
ORIGIN      
        1 aggaattata atttttaaca gggaggggat tactatgaca ttatctgatt acattatttt
       61 tacaactgtt tccgaactga aaaatatgag cctggcttct gataagcttt acctgaccag
      121 gtctgctatc agccatgcca tttcccgcat agaacgtgag ataggctttc cattattcat
      181 aaaaaataca catgggatcg aacttacaga caacggagct gcccttcttc cccttgcata
      241 tgctgttata caaaaccacc agcatttcaa tgaaaaaatc gctgcgatca atggtctggc
      301 ctctggcaat gtccgccttg gaacctgctc cagtatctgt attaactgga tccctgaact
      361 tgtgaataat ttccggcatc attatcctga catacagatc catatatttg caggtattaa
      421 taatgcccag atcgtaaaaa aactggagca aaatgagatc gatattggta ttagttctta
      481 ctactcttgt gatactatca gttcctctgc catcagttct accgtcatct atgaagatga
      541 aatggtttgt gtggcaaatt ctgattttca cacaagtacg cccgggatca ttactgcaga
      601 ggaactgcaa aattctgctt tcattatcag cgatcaggac tatggtgtag aatcccgcag
      661 tgttcttgaa aaacttcact taaatgcaaa ttccactatt acagccactg acgatgccgg
      721 actggttgca atggcatctg cgggtttagg attttgtatt ttaggacgcc tggtattaaa
      781 aggaacaacc tccccggtaa atgtctactc ttttcatcca cgacaattcc gccatattgc
      841 tttgctgcaa aagaagaatg tagccctttc tcctgctgca gaaattttcc agaaacatat
      901 tataagctat gcctcttcct atccaaatcc ggcaattcca ttcaactttt gatgaaaaat
      961 gttcaatggt cctgtatctg acaggtactt gtaaaaaaat cacctgctcc gtataatgaa
     1021 tgtaaagcaa ttaccaacag atcgttcctg agatcttggc aaaaaggggt agcaaaatgc
     1081 agataaaaca agaaaatgac ggacacgcca ttattgatca gctgactgga gaagaagtat
     1141 tgacctgcgc cattaatatt ggagaacaac ttttaatgag tggtgcagaa atcagccgtg
     1201 tagaagatac gatacgcagg atctgtgcag cttacgatat caggcaaagc catatatttt
     1261 ccatcgcatc ctgcatcatc gtgacattag aaacaaaaga tcataaatgg atcacccaga
     1321 cccggcggat attaagctat ggaacagata tgtggaagct ggaccggtta aataatcttt
     1381 cccggctgat atgttcaaca aaaccaccat tagaaaggat caaccgggaa tatgcactta
     1441 tattaaacgg gccggtctat cctcctgtgg tacaatgcct tatctacgca atgacagccg
     1501 gtgcatttgc gatcttcttt ggcggaaatc tattggacgg tttttctgcc atgtttgtgg
     1561 gggcactgat ccggataaca ttgtacgcat ttaccgcaat aaaaatgaaa gccatttttt
     1621 ctaatatcct gtgttccatg atctccggta tggtatgtat ccttacccac tatatcggtc
     1681 tcggacatca tgtagaaatg atcatgatcg gaaatatcat gcttttaatt cccggtgtcc
     1741 tgatgacaaa ctctttccgt gatttcatca gtggcgatat gatcagcgga cttttacact
     1801 tttcagaagc gatcatcact gccatctgca ttgctgctgg atttattttt tcaaaaatac
     1861 tgcttggagg tatcttatga atcaggaact tatctctgtc atttcagcat ttttcggttc
     1921 tctgggattt tctatcatct acaacatcag aggactgcgg atctggatcc cggcaattgg
     1981 aggtgctgtt ttctgggctg tgtatctgat attccttcat tttataaata atgaattttt
     2041 aggttttttc tttgtggcaa tcctgatcac aatttattca gaaatatggg caagaatatt
     2101 aaaaacacct tccaccgtta ttttaatgcc cacagttatt ccactgatcc ccggcggatc
     2161 actatactat gcaatggatg ccgctctccg gcatgatatg ccacaatttt tcttaaaagc
     2221 ccaggctgct gtcggacttg ctgtggcact tgcagcaggt atcatggccg taacctccat
     2281 gcagcattta atatcggcgc ttataaaaat gattaaacca accccgtgac cggaaaatta
     2341 ctggaaaacc ttcaaaaaaa ctatcctatt tattcaaaaa ctcttccaca atggctacaa
     2401 acactgcaga accttcccaa agtacatctt catccacatc aaacttcgga ttgtggtggg
     2461 aaacatctgt tcccttggct gggtttgagg aacttaagaa catgaatgct cctggcagct
     2521 tttccagata gtaagcgaag tcttcacctc ccatgtttgg agcttttact tcggtgatca
     2581 catggtcttc gcctgcaaca gtctttgccg cttttgctgc cagagctgcc atcttgtcat
     2641 cgttgatcac aggcggtgcg ccccagatca tctcataatc tacatttccg ccaaatacag
     2701 cagccgttgc ctgggcgatc tctccgatac gctttgctgc aaactggcgt acttcctctt
     2761 ccagggtacg gatggttcct tcgatcacaa cctcatttgg aataacatta tactggcttc
     2821 caccctggat ctttcctatg gtcagtacca gtggctttgt tgcatttaat tcccgggtgg
     2881 taatagcctg gagagcgatc acaatgtgcg ctgcaatatt caccggatca ataccctttt
     2941 caggtgtaga accatggcag cctctgccat ttaccctgat cacaaactta tcaaaagcag
     3001 ccatgcagca gcctggcgtt actacaacgg ttcctgttgg gattgcttta tcgataatgg
     3061 ttccaatatg ggtgccaaat acagcgtctg caccttctac tccgccattt tcaatcatta
     3121 cttccgcacc tctggactgt tcctcagaag tctggaacat aagacgtacc gtacctgcga
     3181 tctcatcctt atgagcccac agaacttttc ctgcacctaa cagcatagct gtatgggtat
     3241 catggccgca ggcatgcata aaaccttcat gtctggaagc aaatggaagt cctgtctctt
     3301 ctttgatagg aagagcatcc atatctgcgc gcaggcagat ggtctttccc ggctttccgc
     3361 ctttaatagt agcaataatc ccgctgtcct ttttagagca tatataaggg atacctatct
     3421 tatccagctc tgctgttaca taggcagctg tttccggcag atgcaagcca agctccggga
     3481 tctgatgcag atctcttctc aatcctacga tatattcctg taaatcttta cattcattcc
     3541 acatagttat gcaatctcct ctcccactgg ggtctcattt attttttctg cttttgcagt
     3601 gtcatctgca tttccatctt ttttcttgtc acttacatag aacagacgtg ccattccaag
     3661 agtgccaaat acggcacata cgatcaccag ccatgcaggt accggtacaa acagcttcaa
     3721 tacagttggg atcagcaggc caaatacggc tactttcggg tattttactg cgaagtttcc
     3781 aaaagttccg ccgaaaattg cagctgctgc atagtttttt aatgcagagt ttaaaaatgc
     3841 aggaagaaca gctacgactg cagaaccaac taaaacagcg cctgtggttg cgatcaggtt
     3901 tgtgataatg gaaccgcaga tcgccatggt ggaaactact tctgcctgga tggttccgct
     3961 cttgctgtca gttacatcca gtgccagtgc tgcacatgga acacgcatgt ttccaatgtt
     4021 cccggaaagg aaacctaagt atgtaccact cataccaaga gctgcatagt aggaaatcgg
     4081 ctcaacaata tagaatgcgc caaaggataa tgctgtcagt gcccatgctg ccaggactgt
     4141 ttcaagcttt ggccagcagc catagcggct gcacaggtat aataccggga taaatgcagt
     4201 gaacgcagcc atgatacagg ttggtgcgcc aatgcggata ctgtttctct tccattctgc
     4261 catataatct ttcataatct gtcttcccct cctatgctac tgccaggtcg tgcattactg
     4321 caaagaacat tccaatgacc attgcgatac ctaaggaata ttcagctaat tttggatgct
     4381 ttggcacgat cagtttcatg atggctacca tgcttaatgc acctgccaga caggcaatgg
     4441 tgcttccaag accttttttg atctcatttg catttaagta accgaaaata ccaagggaac
     4501 atgctcctga taaaacagcc agccactttg cgtcgccgcc gcttaacttg cctcttaatt
     4561 tctctaatgc cggtgtaact gtaccgctga ctaacagcca tccagcacca tttaacgcca
     4621 ttgcaaacca ggatactgcc ataacggtca gtgtgtatcc gtttcctcca aattcaacac
     4681 ctgctgcctg agcacccatg gttgctgcag aaagctctgt agctgccgcg ccgataatgg
     4741 aaagacgaag ccatgccatt ggtccgccaa tagatgccat aaggccaacc ataacaataa
     4801 atacgccaca ggccgggccg atggctgaga tcagaccgat cttaaaagcc ttgtttgcca
     4861 gtccctgttc cagatttacc tcaaccgctg ctcttttcgc ctgacgcata aacagaagtg
     4921 cctgtgccag agagatcaca actgtgatac cgcacagtgc ccagaagata ggggcatttg
     4981 caattgccag atagtccata gattttcctc ctctaatatc cttcaacctt tttcactggt
     5041 catcttgtat gttgtggacc attatagcat tgagattttt ggaaatcatt gtacttgaag
     5101 gacaacaatt ttcaattaga attgtgtgag gtgtttttta ttgtatgtga gggatagttt
     5161 ggggcttgtt ttatttgtat ctgaaggaca atagatagtg cattcggggt gtatctttgc
     5221 tataataaaa gggtgaagcc atataacata tacaacagaa aggaccttcc catgaccata
     5281 tccgattttt taaaactccc taatttcact gacctgcgcc tgttagcagg caaatacggg
     5341 cttttaagtg aagtgaccaa tgttactgta gtcgatacac cagacggcac caactggtta
     5401 aacggcgggg aactggtgat cactacggca tatatgcttc atgaggaaaa ggaccttttg
     5461 gagtttcttc gcactttaag cagtaaaaaa gctgcaggcg tgggtattaa agagaaccgc
     5521 tatcttacag agataccgga aagcgccaga aaactggcag atgaactgca cctgcctctg
     5581 atctctgttc cggaagctta tccatttgtg gatattatca atccggtact gacccagatc
     5641 ataaaccagc agtcattctt actgacccag gcgaatatga tccataagga atttttaagc
     5701 cttgccatca acaataacag cgtaccggag atcttaatga cactgcgtcg ttttattggt
     5761 attccctgtg cctttatgga tacccatttt aaaaatatct tcttttctga tgaagactct
     5821 cctcttatgc gtcagcttca ggatatggat atggaaaata tttccagtga atttttaaat
     5881 cagtatgaca actacgcggt tgccaacaaa aatgaatcct ttggctatct tctttttgaa
     5941 aaaggtcgcc tggataccgg aaatgaaagc agcgcacaga ttgctttaga atatgccagc
     6001 atcgtcctca tccttcacag ccaggtgcgt attgccaatc agcagatggc agaaaaatac
     6061 aaagcttctt tcctggaaga tcttctgtta aacaatgtaa aagcagatat cgagatccac
     6121 aaccgtgcaa gactgtatgg ctgggacttt acaaacggcg gtctggctgc agtggttgac
     6181 atcaataaca ttaaaaagta ctttattgac cgtttagact ccaacaccaa ccggatgctg
     6241 gaggaagcaa cagaactgat cttccgcaat tccatccacg aaatgcacca gactttccca
     6301 caggccaaat atttccgtca aagtgatctg atcgtattca ttatttctgt accaaaagac
     6361 agcagggaac ttcttgcaga ccagctagag cagaccttta aacggctaca gagccagtta
     6421 tcccatgtaa gcccctttac cattacctta gggatcggac agtattatga aaatatccgt
     6481 gaaatatcaa aaagctactc tgaagccaga gtagccatta atcttggata ttctttgcag
     6541 tggtttgacc gcattttatt ttataaccgt ttaggacttt accgccttct tgccccggtc
     6601 atgaacagcc cggagtcaga ggaactttgc cttcagtaca ttaagccatt ggaagattat
     6661 gataagaaat accatgggga actgctttcc accttacaag agatcttaca gtgcggctgg
     6721 aatctgaaag aatccgctga aaagctgtat atccactaca attccattaa ataccggtat
     6781 tccaagatct gcaaggtatt aaaccttgac ctgtctgacc atgataaccg gtcactggta
     6841 gaaattgcca tgaagctgta ccggctgaaa cattataaaa aggagaacca gtaaagccag
     6901 atgtgtattt atgaccggca taagaacatc ctgccataca gagcagcaaa acagttactt
     6961 tacttttctt ttcaggatca ccatagctaa gaggatcaat ccactaaata gccatgcaaa
     7021 catcgtataa tcctttgatc gccctgtttt aggaagagtc tgaattgcca tctcttcatc
     7081 ttttctttct tctatatctg ctgatccggc ctcttcatct gatgataatg gcactgcgcc
     7141 tgatccttct tcatatcgct gtgatccggg accgatctgc gtataaccgg ttggggtcgg
     7201 attatataca gttccatccc gataccattt cacagcccca ccgccaagca tacgttcagc
     7261 tagggttgct gtatatgcac tgtgttctcc ataaatgcta aatacaagat catctcctgc
     7321 tgcttttcca tctgcgttca ctgctttatt atcaaagatt ccaataccgt ctttgatata
     7381 gagttcagcc ataccagttg cacaaaacca gagaccgcct ccctgctcag ctgtattttc
     7441 agtgactacg gtattatata aatgggcagt gctgtaacca tcaatgcttt tccctctgaa
     7501 tacagtccgc ctccagaata tgttgctgtg ttatttataa tctttccact gcgcagaaaa
     7561 ctgccgttag aaaaactgta aattcctccg cctgtccctg ctctgtttcc ggaaataaca
     7621 cctccatcca taaggaactc tgttccatat tctgctgtgc ctctctgaag tgcagagtct
     7681 acaatacaaa tacctccgcc cttagatgct ttattagcgg atacggttcc gcctatcata
     7741 tgaaatgctg cattatcctc aacgaataca gctccgccag cttcattccc gtttgcagag
     7801 attcctgtat tccgggtgat cagtccgtca tcttccaatg taaatattgc ccgtttatcc
     7861 ctgtcatcac tataaatatt gacagctgcc cctctgtgtc cggtgttatt ctgtattgct
     7921 ccaccagtca taattccacc atatgcagct gcctcattca caacgtccta taatgttggt
     7981 gccccggctt catcggaaat tgcagatcct acagaatcag aaatagattt ttccgtgtat
     8041 gttataactg catttcctga aaatccagct ttggaaatat caactgcatc tgaactattc
     8101 cggatcgtaa ctgtctccaa cgtgccaata ttagaaaaag catcattttt aatactctcc
     8161 agtccgcttc ccaggatcag gtgttcaatg gatgtatact caaacgcatg atcggcaatt
     8221 gaagttacac tatccgggat ctttgccagg gcagtatctt tataaaaggc ctgctggcca
     8281 atttccgtaa tctgtccttt tgctgtcacc tcttccagcg cactacatcc ggaaaatgcc
     8341 gattgcggaa tagatgctac tgaatcagga agtgttatag atgttaaaga aatatcatca
     8401 ctaaataatc ctgatgcctt tttgatctct acaccatccg gaagagaaat atttttcagt
     8461 ttctctgctg aagcaaatgc agctggtccg gttatatatt ttaaactttc cggaagatca
     8521 atggtctcca gagaagaaca atttgagacc atagagttac cggaaatttc ctgtacacct
     8581 tcttcaacgg tgatagtttc aagcttgtcc atattttgaa aattaccacc aaatttcttg
     8641 acggaacctg gaatggtgat ttctgttaaa ttagaaaagc gtgaaaagta tacatcacat
     8701 ttttcaactg tatcagggat catcaatgca ctgacatttt gtctcgaacc tgcaaaaggt
     8761 ccccatctta atattgtaac tgtatactca ttatcttctg cctcatcctc atcataagta
     8821 acctgttctg gaatttctat ttcagaagct tcggccgggt tacttatatc ttttaatgtg
     8881 gcagtatggt catccggatc aagatcaaat gtgtaagtaa taccatcgat ctccatttta
     8941 acatcttcat aatctacagt cagaagtgac atttctttat tctttccatt tttctctgga
     9001 ttgggagaaa tgttcttatc gcttaaagaa tctttgttgc cagaatttcc gttatcatcc
     9061 tttcctgagt tatctgatga atctgcagag tccgtatttt catccggagt ttcatcctga
     9121 tccggcttct gatcctcctg gtcatttcca ctggcatcac tgttatcggg atcatcctga
     9181 tcatccttcg gaccattctg gtcatcttta gaatcatctt ttggatcatc ttttgaatca
     9241 tccatgccgt cgtctttgct tggatttcca gaaggatcac cttctaaatc taaatttcca
     9301 gaatctggat cttcttttcc atagtcatct gtatccgtct tctgatcttc atcttcctga
     9361 ccgggatttt catttggatc ttcattttga ttttcttgag tagatgttat ctctcgattg
     9421 ttaccccatc aaatctatag caaatacaga attaacaggc atagatacaa gcaaactcgt
     9481 agataaaatg catgataata tttttttatt tttcatgatt tccctttcct atccgcaatt
     9541 atacataaaa atagatcaat cccacctgaa caagaaagga cgatcatatt ctcattatat
     9601 aatatacgct ttttttaaca ctaaacagcg atgccgccta atggtgacac cgccatctgt
     9661 gatgttttaa ttcactgata tttttcaatt atcatacctt attgataaac atttgtcaac
     9721 taacccctcc ccatcctatt ctcaggtatt tcgcttttaa gataaaaagt tccccgtttt
     9781 gccagtatcc atctggcaga acggagaatt ctcatattga ataaaaagtt actgtttttt
     9841 ctgcataagc aatcaaaaag gacacctttt attgtataat tatgttaccc acaaaacaac
     9901 acgaaagaag ggggccttaa tgaaaaatta gcattaaaga aactgctaac ttatatgaaa
     9961 agtgtatata aaattccgga gaaaatcaag gggttatcgg acaaaagaaa aagaagatcg
    10021 attccattgt ttaatatcat catgccgtca ctgctcttcc tgatgctgca gtatgagagt
    10081 tttcataccg ttttttctgc gcctgaaagt atgggaaaac gattgaaaaa ctgcatacat
    10141 ggaaagatac ccaaaattga tgcagtccgt gaacttctta cccagataga cccagatgag
    10201 atccgtgaga tccacgagca gacgatcgat atcataaaac gcaaccgtgt atttcgtgaa
    10261 gggactatag gggggatatg ttgtagccgg aatcgatgga gtggaattgt tcaacagcac
    10321 aaaaaagtcc tgttcggact gccttaaccg gaaaaaccgt gcaggtgaaa cggaataatt
    10381 tccaccggag cgtggtctgt atgacagtag gaaaagtgcc acatgtgatc ctaggccagg
    10441 aaatgttaaa accgaaggac ggagcggaaa aagatgaagg cgaactgaca ggaggaaaaa
    10501 gaggtcgttt acctgtggaa aaaagaatat agaattctgg gacctttcaa gatttgagat
    10561 agataatagt ccccataagc ttcgtatgat ccgatatcat gaaagctgga aagaaaacga
    10621 acaatggatg tggctggtaa caactctgaa acaagggaat tctcgggttt tatggaagat
    10681 gatgaaccga agatgggata tagaagagaa cggatttcac cagttgaaaa cgtattatca
    10741 cgcaaaacat tgctattgcc atacagcaac agaagtaata tttgatctga tgatcatcgg
    10801 cttcaacatg agagaattat acttgtatcg ccgtattcaa agatttaaag gaagtgggat
    10861 caatcgaaaa agtatgaacc ggaagttttg tgatgagtta ttgttggaaa aagtaaaaag
    10921 tatcttgtat gaaaagggca gatagtctgt cagtgggaaa ataaaaacag gcgctttgaa
    10981 atgagataag tttagctaag ggggctttgt gcgcattcag ctattcaggt agcaaaagaa
    11041 aaaatgagtg tgggaggggc actatttata aatatacagt gctaaaagcg aaatccctgc
    11101 aactgtggaa tttttattga catttttccg ctggacaata tgccggatga cagaaaaaaa
    11161 ggctaaaaga tgggccggag agctactgct tgtctggatg gtgctttaca actggtatcg
    11221 ctttgactat tataatgaaa aatcgatact gggaacggta ctgcatggct gtggaaagct
    11281 gcttcatatt cccatgaaaa gagcatacgg cttttacgag aagctgtgca aacgctacga
    11341 tgaaaagccc acccgctttg tctgcgatac ggtgtttata cggtatctgg agaaaaatac
    11401 atgggagcgg agctggtttg atgcggtagt gtatctgccc tttgaaaacc ggattattcc
    11461 ggccccgaca ggctttgacg gcaggctgcg gacggagtac tgggattaca tgaaaccgtc
    11521 aaaggcaccc accatgcatg gagggctgat tctggagcct gatgtgtcct atgtggagta
    11581 tctgaaagaa aaaatgccgc ggcagaaata agttctgccg cagcgcaata atcagattcc
    11641 tccgagcata cagatgttgt tttcaatatt tcgtgcatat tgattgcgaa gataaaaggg
    11701 aagctggtaa cgtttttttg cagtttcgga atcaacagcg tttttatcgc gaaaaatgcg
    11761 gatagttccg tcgggatagt aaagcataac ctgttcaccg ggaaaattca gaattttggt
    11821 attcatggcc acttttccgc ccagatggca aatccggttt ccatcacgat caattagctc
    11881 cgtatcccct gctgccacat tccgcaggat ttcatgcagt ccgtctccgt ttatatcgat
    11941 gggagcagtg tcaaacagac tgtaaggaag agaaatgggg gcccctgtca tggcatcgta
    12001 gacaaattct gtaattccgg tataataacg gccgaaagca tccttggatt gtcctgtaat
    12061 gcgcattcca ttggccagaa gccggccttc ctttccgatg cgtccaaccc agcctttgtg
    12121 aatatgcccc cagtctacat gggtccagat gggctgtccg ctggtgtcgt acattccgat
    12181 tcgcggccca atctgctcat tcgattcgcg gttgatatag ataagccaac ggttgctgtc
    12241 ataatcaaac accggctgac acatatcgga atgaccgttc catgtgtctt tttcgatgca
    12301 gaataattcc gaaccatcat gaagggagag gcatcgctcg ccccacagta cctcgtcaca
    12361 gccgtcattg ttgatatcta caacaggaaa ggaatggctg cctctggctc cgggcgcatt
    12421 ttccccaatg gacagagtcc acttggaggt catatcggga ttccaggcct gaaagtacat
    12481 gtctgcgtaa gtgccctggg cagttacaag aacaggctga ccatggtcat agcccccgaa
    12541 aacatgattt cggaaatacc acatggattt ttggattccg ccatagttgg gccacggcca
    12601 ctggccggtt acatttcctg taagcacgtc catgcgttcc agcacataag cttccaccca
    12661 gagaggatga atggggtcag aattatttac aatataaatt tcatcaatcc catcctgatc
    12721 catatcaatc agtgtaaaac agtacagtgc tgcgctgggc cagattccca gttccttttt
    12781 ccaaaggact tctccgtgct catcaaatgc aatgccatgc agtgtgtcag aaggaacaac
    12841 aaacatgctg taatgcacat cgacatcgta gcaggcggaa taaaaagcga caaatccctt
    12901 tcggccgttt cctaatttgg caggcattac tttatattgc agtgcaggag agcctaagtt
    12961 taaatttaaa gcttcttcaa tttgaatttc catattttaa cctttacatt aagcgatagt
    13021 ttattgtgcc tggtattcag cgtaagcctc attcatctgt tccgcccagt tatcaagaaa
    13081 actctgaaca gacatttctc ctaaaagcat agcctggaag ttagcaggat tgttggtatt
    13141 cataatggtt gaataatccg gcagatactt tggctgtgaa accaggtggc agttgcttgc
    13201 agtaagtgtg tttaaagctt ccatggtgga ctgtgattta gatacccagt cctccttcag
    13261 caccttgttg ttcatcggta tctggccgat ggacttgttc cagtagctgt tggaatcttc
    13321 cgagagcata aatttgatga attcccaggc ttcatccgga tgttcgcagg tattgaaaat
    13381 acagaaaccg ccctggcttc cggctgtgta ggagcgggtt ccggtcagag attcggggaa
    13441 ggaaacggca acgtactggt ctggctgaag agcagaggcg tgttcggctg cggaaccggt
    13501 attgtggaat atcattgctg cagagccgcc gtcaaatgcc gctaccattt ccttgtagcc
    13561 gttggtaata tcactttccg cagtatactt gttataaaga gcggcatagc gggttacaaa
    13621 ctcaacgtgt tccggtgcgt tgatggtgca ttttccattt tcatcaaaat aatcttcaat
    13681 gccggaatat gcatacatgg catgctggag ctccacgcag ctgctggaat caccgcggaa
    13741 agaaaagccg tagacaccct tggatgcatc ggtcattttt tcggcggttg taaagaagtc
    13801 atcccatgtt ttcggtacgt caagtccttt ttcttttaat aagtctgttc tgacccacat
    13861 taaaggggta gatacggtat cggtaatcat aaaaagccgg ttttccgggt caagctcacg
    13921 attcaggcgg agataatttt cattgatgtc gcctttttcg tcccaggcat caaagtaaga
    13981 atccagatct accaggcatt cctgggcaag ccagttggag ccccaggcag cgttgcagtg
    14041 gccgatatca ggagtggcat tggtagcaat ggctacatcg tatttctctt tggcagagga
    14101 ggaggggatt ccttcgtagg ttacgacaat ccgatcctgc gagtcattga accgctttat
    14161 cagctcttcg tagtaaggcg ttcttttttc gcctgcattt tcgtcccaga aggttaaggt
    14221 aatcttttcg tcggcagttt ttgcttcttt ttcgctagct gtctcagcag tgctgttttg
    14281 ggcagagctg gaagtctcct ctttggtagc ggttgtatcc tttgccggag tggaggaaga
    14341 agatgaacca caggccgaaa gacagcagag agctgttgcg gtcagggtaa atccaaggta
    14401 acgggttgct tttctttttc tcataatgtc ctcttttctg cgctgctttc ttttgttggg
    14461 gcacttctga ttataccgca gcgctgatat aatggaaatg ctttatggtt atcctttgac
    14521 ggctccggaa cttaatccgg aaaccatgta cttctgaacg taggcaaaca taatcaggat
    14581 gggaagcagt gcaataacac ttccggcaca gagctgcccg taattaatgc tgtattcacc
    14641 gatggtcatg ctgagtccca cgggaatggt gtatttgctg tcggaactca taaacatcag
    14701 cgcgtagaga tattcattcc agctgctgat aaaggagtag gaggagacag cagcgattcc
    14761 ggggagaagc agcggcatca gaatgtgtgt taccgcctga agacggttgc atccgtccac
    14821 tctggcagcc tcctccagcg tggcagggat tccgcttata aagctccgca tgagagctgc
    14881 attaaatgga atgtggaatg cagtgtaagt aaggattagg gacatgtgat tgttaatcag
    14941 gtgggtcatg ttgtaaattt taaacagagg aataatcagc atggaggttg gaaaaaactg
    15001 agtgcacaac agaatcagca aaaatgcgtt tttccctttg tatttatagc gtgccagtgc
    15061 ataaccgtcc atggttgcaa aaaggacggt aaaaacggtg ctgataaggg aaaccttcag
    15121 gctgtttaaa aaatagcggg aaaagccacc ggttttccag gcattggagt agttttccgt
    15181 aataacatgg actggccagt aagtaattgg cttggctgca atttccgcac tgcttttcaa
    15241 agaagttata aaggtccagt aaaatggaaa aagtgtccag ataactgcgg caagcaggat
    15301 aagataaatg caggtaagtt cacggcgttt ttttgaaatt gttttcatgg cttatctatt
    15361 cctcctcatt tccaaattta ctcagcttta gataagtagc tgcaaaaatc agcagtatca
    15421 ggaaggaaat aactgccaat gcgcttccat aaccgaaatt gccgtctttg cgggcggtat
    15481 ttgccaggta tacagacaaa gtggtggttt tgtggacagg gccgccgccg gtcagattga
    15541 gaattaagtc tacgctgttg aattcccaga tgacacgaag taaagtggta aggacaaggg
    15601 tttctttaat atgtggtatt gtgatgtaaa agaaactctg cagggcattt ccgccgtcta
    15661 cgcggcaggc ttcgtacatt tcatccggaa cggattgcag tgaggcgaga agcataatgg
    15721 agaaaaaagg aatgccgcgc catagctctg cgataatcag agaccagaag gcagtatgtt
    15781 tgttccctgt ccaggcaatg gctttctgta taattcccag ccgctgaaaa agatcgttta
    15841 aaactcccac attttcatag tacatcatgg accataacat agagacaagt acgccggata
    15901 atgcccatgg aagaaaaagg atactgcgga agatgccgca gccccggaat ttccgcttca
    15961 gtatcagaga gagaccgaga cccagaacaa gctgaagtcc tacttctacg atgacccagc
    16021 gaaatgagtt gccaagggct tcaaagaaaa ccgggtcgga aagaatttta tggtaattct
    16081 ttaatccgat aaaggcattt gctttgggac gggaaagcat ttcctgccaa aagctggtat
    16141 agaacacatt gaaaaccgga taaacaagaa gccagcataa aagaagaatg gttggcagca
    16201 ttaacatata aggaataagt gtaacagggc gcttgtgatt aggaaaatat gttttcacag
    16261 gtaaatacct cctttgtgat atatattaaa acaaatttta tggaaaatat aaattcaaaa
    16321 tctttgtgaa aatgttcggt atttttggct tttgcaagat tcaaatggac agatagccat
    16381 aaaagaagga taatgactat ataagcggga aaaggagaag gcggatgaaa aagaggttta
    16441 aggaaatgtc gctgcgttct attatcatga tattatgttc atcctttgtg tcactgataa
    16501 ttgttacgtc ggccactttt tatattacag gcatcagcca cattaataat ctggtgaagc
    16561 agaacgctgc aaatcttacg attcaggcag cagaaaatat ggacaagcgt tttcggaagg
    16621 taaaggattc gttcttcagc agtattatgg aatcctacta ttttctgaaa atgggtcaga
    16681 atattatgga aaacaggcag ccgatttcgg caaagcatta tgcggagctg gcaagaaaaa
    16741 tccaggatta tgtaaatcag aacaggcagg atgtcagcgt tgttattctt atgctctcca
    16801 ataattccat tgttctgact gcatcgggcg aagaagaatt ttaccggtgt tatcagccgg
    16861 aatacgatgc tttttacagg aaatatgagg acggaaagct gaactggatt tacagcggtg
    16921 acgcggcagg ctttatgggc aattccgggg agataccaca tcttgggatg attcaaatgc
    16981 tgggaagcag agattctgat atacatggtt tcatatacat tggcatgtca gataagggaa
    17041 ttgcccgtga attaggcagc taccatatta caaacagcaa tagtctggca ctgatgaaag
    17101 aaaatacatt ttttctgaca aaagatttat cagcaggcac tgcggatatt gatttaaagg
    17161 agaactatgt tgtaagtgaa catttgaaag aggttcccct ggacgttgtt gcaattatac
    17221 cgaaaaatga attgtttata gacagaaata attttcagat cctggtgttt ggagaatcag
    17281 tggtatcggt actgttctgc ttccttttat ttatggtgat taccaaggta ttgtcggcac
    17341 cggtggagga gctcgtccgg cagctggatt cagtcgaaaa cctgacacag ttagagtcag
    17401 cccgcgctgt gggaggaaaa gaatttagaa agattacgga ggccattaac catctgtatc
    17461 agagaataca gatactgata acgtccttaa aggaagaaaa ccagtaccgg aaagatgcag
    17521 aattaaagat tctgtatgca cagattaatc ctcatttcct gtacaataca ttggacagta
    17581 tcggccagct ttgcgaaatg ggagaaagtg aatcggcctg tcaaatggtt tatgaactgg
    17641 cggattttta tcgaattggg gtaagcaaag gtgcggattt cattacattg gaggaagaaa
    17701 tccggcatgt aacagcctat ttatccattc ttcagactcg ttttgaggat ttccattttc
    17761 agatcgaagt accagaagag cttgcgggca catacgttcc gaaaatcata ttgcagccgt
    17821 tggctgaaaa tgcggtttat catggtattc acagcggaaa tggggatgga aacattcgta
    17881 taaccgtgaa aagagagggc gaagatttat ttctggagat ttcagataat ggaaatggaa
    17941 ttgaggagga gcttctggga aaaataaggc gttcccttca gctggatgag gtaaaagcgg
    18001 agcaggatgc aaaaatatat ggcttaaaga atgtacagca gcggattgtg ctgcaatatg
    18061 gtgcgcagta cgggatttcc attttcagca tgctggagca ggggaccagg gttttgatta
    18121 aattgccata tttgacgcag atatagaagg gatagacaga gatgacaaaa atattgtttg
    18181 tggatgatga tgccattgcc aggcggaata tacggacaaa aattgactgg aagtcctata
    18241 actgggagct gatttatacg gcaaaggatg cagtggatgc attggatttt atgaaacaga
    18301 atcagccgga tattattctt tccgacatta aaatgcctat tatggacgga atacagatgg
    18361 caatggtggc gagaaactat tatccggata ttaaatacat tttcatatcg ggttataagg
    18421 aatttgaata tgcaaagcag gcgttgaaat taaatgcggt ggattatctg aataagccgc
    18481 tggaaacaaa gcagctggtg caggttttga gagaggcgga gcggctatcg aaagaggcaa
    18541 aacgaaataa tgatatttta agggataagt atccgtttct gcagcgccat tatctgtcac
    18601 aactcatgta tcagcatttc agggaaattg atgcagcagt atttaaagcc tttgacatta
    18661 atttgtccga tggctatgga attgcgggat atttacagat ttgtacggat gaaaatagtg
    18721 caggaacaga cagggcagaa aaactgggac gctatctgtc ttccatatat gaggggagtt
    18781 tctttataag tatgggagta aaccagattt ttctgattta tacctgttcc aatgtggaga
    18841 atgaggcaag ctttaaagtg cggatagaaa agatggaatg gcaggcacag caatatatga
    18901 aaaaaggaga aacactggtt tttcattacg gaacagtgat acgaaatctn nnnnnnnnnt
    18961 aaatgagctg tatgtgtcct atgaaagtat actgaaaaaa atagatatgg ataccaatga
    19021 tttactggca aaagtaaagt cctatctgaa tcagaattat tttgtggagg atttaagcct
    19081 gacgcaaatt gccgaatatt ttcatgtgaa ccattgctat ctgaccagta tttttaagga
    19141 aaagtatgga atcaatctct acgattatct gattcagacc agaatgaaaa aggcaggaga
    19201 gcttgtttgt tccacaagta aaaaggttta tgaaatagcg gaggaagttg gctataaaaa
    19261 cagcaggtat ttcagcgtag tatttaaaaa atattttggc tgtaccgtgc tggaatacag
    19321 aaaacagcat acataagaga aagccctgac agcgatttgg gatatcccca gttccctgtc
    19381 agggcttttt aaactgcgat ttgcgtggca gctatggcca caggctatgt aaaaaattta
    19441 ctgattctcc ggcaggttca tatctgttgg gtcataatac tgatccatgg aaatcagagt
    19501 cttgatggcc gggtggtcaa atgggcttgg caccggagat tcgtatacgt ttacggttgt
    19561 ctttaacggg cagtgctcgc aaatccaacg ggagtctgct gtggtgaagc agatgcagcc
    19621 gtgggagcgg ttttcgccca gccagttgta ggtggacggg aacagagtgt aagggttctg
    19681 cgtctcgtaa ataacgtgta tcatcccaaa tcagatttta ttttaaaaaa caacctccat
    19741 actatagaca tatcagaagg aggacaaaca ccatgacaga acaatcattt acagccgagc
    19801 agttagagca ggcagccaga gctattctaa tcgatcacaa atacagcatc tcagacatca
    19861 aagaccagta taaacagcaa atctatgaag cattaattaa actctcacca gcctttgcat
    19921 taagctacat accagaatca gaaagaaccc catacgctct taaatatttt tcgcagaatc
    19981 agattataga agatttatgt gatgatgcaa ttcagagaaa cggggattgc ttagcattta
    20041 tcctaaatcg atataaaaca ccagatagat gcctcacagc catcggcaat gcaggctatg
    20101 aaattttaga ctacattcca gataagtaca aaacagacga gtcttttatg ctggaagaaa
    20161 ttgcaagaga cgagtacagc cctcttaagc tcgatcaaag catctgggat aaaccaaagc
    20221 acttttggat acaggctatc aacaaaaatc cattatccat agttatggtc ccgaagcaat
    20281 acaaagacat ccacatgtct acaatagcag tagctatgaa cgctgaagct ttagcatata
    20341 tcccgaaaga tggctggaca aaagaaagga gcaatcccat gaacaatttt caagtatctt
    20401 ctatattaat cagggatttc gcttttagca ctgtatattt ataaatagtg ttcctcctgc
    20461 acgcattttc tcttttgttt cctgaatagc tgaatacgca caaaatcccc cttagctaaa
    20521 cttagctcat ttcaaagcgc ctgtttttat tttcccactg acagactatc cgcccttttc
    20581 atacaagata ctttttactt ttttcatctt gttcttttgt ccgataaccc cttgattttc
    20641 tccgggattt tatatacact tttcatataa gttagcagtt tctttaatgc taatttttca
    20701 ttaaggcccc cttcttt
//
LOCUS       DBMDUA010000024        20709 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1595_length_20709_cov_2.962927, whole genome shotgun sequence.
ACCESSION   DBMDUA010000024 DBMDUA010000000
VERSION     DBMDUA010000024.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 20709)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20709)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20709
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1595_length_20709_cov_2.962927"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="ACZPCL_03435"
     CDS             <1..381
                     /locus_tag="ACZPCL_03435"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF014622.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009229 - thiamine diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR1161573.1"
                     /translation="TLILHRFPEVVHHLGYDKLHLPLEMLKTIGRPEGLTLLGTSIHS
                     VEDAKEAERLGADYVFAGNIFETDCKKGLPGRGLEFLENVCKEVDIPVYAIGGITAEK
                     MPQILGTGAAGGCMMSGFMQLKEK"
     gene            complement(419..1303)
                     /gene="thiM"
                     /locus_tag="ACZPCL_03440"
     CDS             complement(419..1303)
                     /gene="thiM"
                     /locus_tag="ACZPCL_03440"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF006830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HIR1161574.1"
                     /translation="MDNRNGNINKAQLLSCLKQVRDQLHDQAPLIHCITHPIVVNDCA
                     NAVLALGGRPIMAEHPAEVADISASAAALTVSLGNITDARAESMLLAGKAIKRMKDAE
                     NAKRASVIDLVGITCSPFRMELAKRFIKECKPAVIKGNGSEIRAIAGAAFHGTGVDVS
                     AADAVTAKDPDTVHSMAHIARKLAEETGAVILVTGEVDIIASPNKDTTYGIYNGSPNM
                     AKVTGTGCMLTCITGTYLAVTDALTACILSAITLDCAGECANAAKGLGTYHIELINQL
                     SVMTEDQITQLSNIQRLL"
     gene            complement(1401..2117)
                     /gene="thiW"
                     /locus_tag="ACZPCL_03445"
     CDS             complement(1401..2117)
                     /gene="thiW"
                     /locus_tag="ACZPCL_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HIR1161575.1"
                     /translation="MKNSKTHVTKLVFLAMMVALGVVISPILRVEGMCPMAHFINVTC
                     AVMLGPWYAFVCALAIGIIRMVCMGIPPLALTGAVFGAFLSGMLYRLSKGKLVCAFIG
                     EVIGTGIIGAIVSYPVMTLIWGRTGLTWFFYVPSFIAGTLIGGSLAFIFLKHLQKAHM
                     LSTFQTALGSQVYNNTDTVVNDSLGIAFLGFIGYLASTVAVKQFVAEPGPVAGSIKYI
                     VLLAFVAMAVVYWNTNKKHA"
     gene            2570..4255
                     /locus_tag="ACZPCL_03450"
     CDS             2570..4255
                     /locus_tag="ACZPCL_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5722 domain-containing protein"
                     /protein_id="HIR1161576.1"
                     /translation="MKNYKRSGAAGFFCAAALFLGTGVLALGTSAFNALAAEVSGQIT
                     SCKITDDKQNVEIALNSSGSTEGTDGKVYVFEQPTYQDDLGSRSDYLTSANASGATTV
                     TVPFNKGDGSDCLYSKFVLAVKEDGTYKAVSEPHYITNPEIVAKNTEAFKEPLTKKGL
                     NIELNMLDDAFDLGVKYVTTNIAVSRLMGSGIDFQYEGKTYHFNKGIVEDYDKVISAY
                     SGKGMVVNAILLNDWSDTTSNLFIPGVQKTSDAYYYMFNATNEAGFEQLKAISAFLAD
                     HYSGKNANYGKVSNWIIGNEIENQEWNYMGPMDLTNYVKTYEKAFRVCYTAIKSTNAN
                     DRVYLSLSYNWMNEMDGQLKYGGKEIIDSFNSIANNQGQMEWGLAYHPYPCPLIDPVF
                     WDDAQATGLVKNDFNSPVINFANLNVLTDYFCQEALKTPSGHVRHIILTEQGFTAYSP
                     TRGDVPELQAAAFAYSYYLVDSNPYIDAYTLSRQVDAPSEAKDGLKLGLWECDMSKPN
                     LIEATKRRKIWQVFRDIDKKNSTLEASEFAKSLIGINKWSDVVPNFKWKNLEK"
     gene            4430..5641
                     /locus_tag="ACZPCL_03455"
     CDS             4430..5641
                     /locus_tag="ACZPCL_03455"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR1161577.1"
                     /translation="MGLIVQKFGGTSVADTERLRNVARIITDTYKAGNQVVVVLSAQG
                     DTTDDLIEKAKEINPEGSNREMDMLLSTGEQISVALCAMAIEALGYPVVSLTGWQAGI
                     LTDTAAKNARIKKIDTERLEAELDQKRIVIVTGFQGVDRNQNITTLGRGGSDTSAVAL
                     AAALEADLCQIYTDVDGVYTADPRHVKGARKLDEVTYNEMLELATLGAQVLHNRSVEL
                     AKKYNVKLEVLSSFTGHPGTKVKGVAKRMEKTAVSSVAKDKDIARIALVGVPNEVGTS
                     FKVFSLLAQNHINVDIILQGIGHEEGKDICFTVAEGDLKKAAGLLESHKAELRFARLE
                     TNADIAKVSVVGSGMINNPGVAAKLFEALYDAHININMISTSEIKISVLVDKKDADRA
                     VQAVHDKFFAF"
     gene            5799..6428
                     /locus_tag="ACZPCL_03460"
     CDS             5799..6428
                     /locus_tag="ACZPCL_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HIR1161578.1"
                     /translation="MYTCAKCNVLACGSQDPAVREKMPKNCPMREKEKMDGAYEKYVL
                     EENHEFYVSCSEIEGEGYCQWPRLKETVEFCKKMGYKKIGLAFCRGLRKEARVVADLF
                     TAQGFDVVSVICKTGGFPKESVGIPKEKKVNADAFEPMCNPIAQAEFLNSQETEFNIA
                     LGLCVGHDSMFYKYSKQMVTTLVAKDRVLAHNPCGAIYCAEGYFKKRLQ"
     gene            6497..7102
                     /locus_tag="ACZPCL_03465"
     CDS             6497..7102
                     /locus_tag="ACZPCL_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012610.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1161579.1"
                     /translation="MEYIGNKKKQGSIVELLRDEILAGNIPDGSEMTQNELAESLGVS
                     RMPVREALILLEYQGLIDRLPNNHVRVASLTEDYFRHILKTGCRLELEFLQNLEEEER
                     SSLPDRELNFHREIWRNYPYSLSRKNLETITEIYLYFVISRRNPEGVWGNVREKTEEE
                     ILLSKSIENWKAGKTEEGAGQLEEYFEKMAVKLAAIHKGRD"
     gene            7109..7750
                     /locus_tag="ACZPCL_03470"
     CDS             7109..7750
                     /locus_tag="ACZPCL_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1161580.1"
                     /translation="MVTLKPIKLLPARERVASALRKAIISKQIGEGEVITLEATAQQL
                     GVSVTPVREAFQILARDGLIDLKQNKGATVLGLNETTIREHYEIRAALESAACVLCCQ
                     RKADLSAIRNCLEMADGALEKKDSSSYSNYNQSFHYEIWTAAGNEKLKTMLSEMWNGL
                     SMGLKSTEEEYARNSQEEHRQILAALESGDPKQAGEKMVFHIIRSMNDVLTRY"
     gene            8064..9329
                     /locus_tag="ACZPCL_03475"
     CDS             8064..9329
                     /locus_tag="ACZPCL_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR1161581.1"
                     /translation="MSPSTITILFLLFAIVMFVMEKIPLGVTSMIVCVGLVVTGVLDA
                     KTAFAGFIDSNVILFVAMFIVGGAFFETGMANKIGGIVTKFAKSERMLIAAVMIIVGV
                     MSGVLSNTGTAAVLIPVVIGIAAKSGYSRSRLLMPLVFAAAMGGNLSLIGAPGNMIAQ
                     SALSEIGLGFGFFEYAIVGLPILICGTLFYTTLGMKLLPDRQPKDDGAFDTTADYSNV
                     PAWKQKLSLVILLLTLLAMIFEKKIGIKLYVSGCMGALALIITGVISEKQALNSIDLK
                     TIFLFGGTLSLAAALEKTGAGEMVAEKVMHLLGENPSPYVLTFAVFMLCCIMTNFMSN
                     TATTALLVPICVSIAQGMGADPRAVLMACVIGGSCAYATPIGMPANTMVLGAGGYTFL
                     DYTKSGLPLIVIATIVSMIILPIAFPFFP"
     gene            9420..10319
                     /gene="ttdA"
                     /locus_tag="ACZPCL_03480"
     CDS             9420..10319
                     /gene="ttdA"
                     /locus_tag="ACZPCL_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HIR1161582.1"
                     /translation="MSRTEQVEELTNYMAHFVAHIGKKLPDDIIAKLDELAEKEDSPL
                     SKVIYQTMKRNQDLAVKLNRPSCQDTGVLQFWVKCGTKFPLIDDLEVLLKEAVVRATV
                     DAPLRHNSVETFDEYNTGKNVGKGTPTVFWDIVPNSDKCEIYAYMAGGGCTLPGKAMV
                     LMPGAGYEGVTRFVLDVMTSYGLNACPPLLVGVGVATSVETAALLSKKALMRPLGSHN
                     ENERAASMEKLLEDGINEIGLGPQGMGGKYSVMGVHIENTARHPSTIGVAVNVGCWSH
                     RRGHIIFDKDLNYTITTHSGVTL"
     gene            10319..10945
                     /gene="ttdB"
                     /locus_tag="ACZPCL_03485"
     CDS             10319..10945
                     /gene="ttdB"
                     /locus_tag="ACZPCL_03485"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HIR1161583.1"
                     /translation="MLEIKDGKKILTTPISDEDLKDIHIGDIIYLNGNLTTCRDVAHR
                     RLVEEHRPLPVDVRNNAIFHAGPIIRPLGDDKFEMVSVGPTTSMRMEKFEKEFVEQTG
                     VKVIVGKGGMGPNTEYACKNFKAIHCVFPAGNAVVAATEVEEIVRAEWRDLGMPETLW
                     NCRVKEFGPLIVSIDTEGRNLFEENKVVFNERKEKVYEEICKHVSFIK"
     gene            11196..12647
                     /locus_tag="ACZPCL_03490"
     CDS             11196..12647
                     /locus_tag="ACZPCL_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR1161584.1"
                     /translation="MSNSAQTSASSRIAALLDENSFVEVGAYIAARNTDFNMTEQETP
                     ADGVVTGYGTIGGCLVYVYSQDAAVLGGSMGEMHAKKICNIYSMAMKMGAPVIGLVDC
                     AGLRLQEATDALDGFGKLYLSQTMASGVIPQIMAIFGTCGGGMAVSAGMADFTFMEDT
                     SAKLFVNSPNALEGNYKAKCDTSSAKFQAEESGLVDFTGDEASVLSQIRTLVSILPSN
                     NEEDLSEVDCTDDLNRMCAGIEGYTGDTAAALQMISDNNFFMEVKKNYGASMVTGFIR
                     LNGSTVGCVANRSEVYENGEKVQTLEAALCGKGCEKATDFINFCDAFSIPVLTLVNVN
                     GYRATMCSERKIAKLAAKLTYAYADATVPKVTVVVKNALGSAGLTMGSKSLGADVVYA
                     WPNAVIGTMDPAEAVKIMYAKEIEAADDAVAMIKEKTAEYTAMQSGAVAAAKRGYVDD
                     IIKAEETRQRVIAAFEMLYTKSEDRPSKKHGTV"
     gene            12665..13480
                     /locus_tag="ACZPCL_03495"
     CDS             12665..13480
                     /locus_tag="ACZPCL_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HIR1161585.1"
                     /translation="MRRFVKRMAAAVLLSACLVSLSGCSSNQDTAETDSTISMDGTPV
                     DDSMAQSIMLSAAQTLGVPKDQLIVQKTLAESTGDTTTAAIYEAQLEVREDMGELKNV
                     NMDEGSVVLLADGSYTVVIPVDFTEGTKKYVMNINMATQQIQAEFTDMSAGVVEDTSM
                     GALLKTATVYTIIGIGTVFLVLIFISILISCFKYIHAWEESKKKAAAPAPTPKAAAPA
                     VKPVAAAPAVTGPDLSDDAELVAVMTAAIAAYEGSATSNGLVVRSIRRVGLGR"
     gene            13542..13922
                     /locus_tag="ACZPCL_03500"
     CDS             13542..13922
                     /locus_tag="ACZPCL_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR1161586.1"
                     /translation="MKTYTITVNGKAYAVTVEEGAAAAGAPVAAAPVAAPAAPAPAAA
                     PAPAAAPAGAAGSVQVTAPMPGKVVAVKASVGQAVKKGEVVLVLEAMKMENDIVAPED
                     GTIASINVANGDAVESGAVLATLN"
     gene            13973..15118
                     /locus_tag="ACZPCL_03505"
     CDS             13973..15118
                     /locus_tag="ACZPCL_03505"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HIR1161587.1"
                     /translation="MDFGNLVSNLLGQMAFFHLSAGNYIMIGVALVFLYLAIAKGFEP
                     LLLIPIAFGMLLVNIYPDIMYSPEQTSNGVGGLLYYFFTLDEWSILPSLIFMGVGAMT
                     DFGPLIANPISFLMGAAAQLGIYSAYFFAILLGFSGKEAACISIIGGADGPTSLFLCS
                     KLGQTQIMGPIAVAAYSYMALVPIIQPPFMKLLTTDKERKIKMSQLRSVSKLEKILFP
                     IIVTIVVCLILPSTAPLVGMLMLGNLFRECGVVKQLQETASNAMMYIVVILLGTSVGA
                     TTSAEAFLNATTIKIVILGLVAFCVGTIGGVLLGKILCKVTGGKINPLIGSAGVSAVP
                     MAARVSQKVGAEYDPTNFLLMHAMGPNVAGVIGTAVAAGTFMAIFGV"
     gene            15183..16595
                     /locus_tag="ACZPCL_03510"
     CDS             15183..16595
                     /locus_tag="ACZPCL_03510"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HIR1161588.1"
                     /translation="MAEIEKKPVKIVETVLRDAHQSLLATRMTTEQMLPIVDKMDKVG
                     YHAVECWGGATFDACLRFLKEDPWERLRKLRDGFKNTKLQMLFRGQNILGYNHYADDV
                     VEYFVQKSIANGIDIIRIFDCLNDIRNLETAVKATKKEKGHAQIALCYTLGDAYTLDY
                     WRDIAKRIEDMGADSLCIKDMAGLLTPYKATELVKALKEGTTLPIDLHTHYTSGVASM
                     TYMKAVEAGCDIIDCAMSPLALGTSQPATEVMVETFRGTPYDTGYDQKLLGEIAAHFQ
                     PIREEALKTGLLNPKVLGVNIKTLQYQVPGGMLSNLVSQLKEAGQEDKYQQVLEEIPR
                     VRKDFGEPPLVTPSSQIVGTQAVLNVLMGERYKMVPKESKKIMLGEFGQTVKPFNPEV
                     QKKIIGDETPITCRPADLIAPQMPQFEKECAQWKQQDEDVLSYALFPNVAKEFFQYRE
                     AQQKKVDATVADKENKAYPV"
     gene            16749..18068
                     /locus_tag="ACZPCL_03515"
     CDS             16749..18068
                     /locus_tag="ACZPCL_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1161589.1"
                     /translation="MLKVLIADDEEKICQLIIKLINWEEMGLKIAATASNGIEALEQA
                     KICKPEILITDIRMPGIDGMELIRKVKEKLPDTEIIIISGYRHFEYAQTAIRYGVRNY
                     LLKPIKKEELRDTLQKIADIYREKNEQLNFEERVRLALKNDAGKLRTSFISRMIYGDR
                     ERENIISLKELNNQYHFHFREGVFQVAAIKFDHVNHNDSCISFLADKAAGLALQYLEP
                     VCFDHEVYAEFSTFYLLLNFGEEESKNVRKNVRQMLDELKAQESILNGMSVTIGMGSM
                     VNTAGSIKKSFLDAVFMIKQRLLLGTGRMLEMDEAQQRKNSDFINSEAFYRFNREMER
                     AVEALNVVETRRTIEGLRDVLKNYPGITGYEILQMTKEACNHYLFCMKSRGIRVDSEA
                     KFVEEFSKDAGECADIEELFRLLGGTIGRSLQKASEAKKAEDNRPIR"
     gene            18081..18368
                     /locus_tag="ACZPCL_03520"
     CDS             18081..18368
                     /locus_tag="ACZPCL_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012392.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1161590.1"
                     /translation="MEENYSRSLTLEEVSEMAGFAPGYFSTLFKKETGVTFLEFLQSV
                     RMDAAKKLLVSGNEGMNVICEKVGYADVKYFTKCFIKYTGLKPGEYRKIYS"
     gene            18479..19375
                     /locus_tag="ACZPCL_03525"
     CDS             18479..19375
                     /locus_tag="ACZPCL_03525"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1161591.1"
                     /translation="MKWFILFLGVLYVILLYAAYQLIYRPLKVNSRIKAAFARGDSYE
                     DIYHMPYLLNEEEQNMLRRFYALLDKSELITVSKKKSEYLALQNQINPHFLYNTLEGI
                     RAEALIAGVDSIAEMTEALATYFRYTISQVNNLVTLEEELANVENYYYIQQFRFGSKL
                     DLRIRYDCEDEEEALMCQLPKLTLQPIVENSIYHGLERKIGTGHLDIKVTVTDSHLII
                     MISDDGVGIEKGQVKVMNEQLRALRLEEENNGETGKKGGIAVKNVNNRIKLLFGEEYG
                     IYIYSQPDVGTDVEITLPVVRN"
     gene            19409..>20709
                     /locus_tag="ACZPCL_03530"
     CDS             19409..>20709
                     /locus_tag="ACZPCL_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1161592.1"
                     /translation="MKQEILRFENVTCKKDGAVYLDNFSFYMTAGEIVGLLSVNDRGI
                     KELIELMAKNHPIDAGRVYMGGKLVNSYLNQEETSNRIYQIDQKSSLINDLSITDNLF
                     VMRPGFKKQVINEWVLREQADRVLKQLSMDLDLNKRVERLTTMERIMVEIAKAYLMGS
                     KLLVVLYPEKLIGQADFPQFHQLLRKMQSMGISTLYFCYHHWIMFKICDRIALFSRGR
                     IKKLFEPEEFREEAIAPYIYYFKENTVFKPKVDRGYMLELKNITDGDIHNLSLGIKPG
                     ECITLLDSGDQIMDRLTGILAGEYVDYEGDIFCSHKKMEKKVKSSLDRGMIVLDDNPA
                     QSFLFSEMSYLENLTFLLDRKLKKSILKQSYINSVRSEYYGEAGDVIDTKYIDSLSLK
                     EKYGLIYNKISLFHPKILVVKKPFAYGVRHHVGALGPGHWK"
BASE COUNT         6232 a         3993 c         5134 g         5350 t
ORIGIN      
        1 acccttattt tgcaccgttt tccagaggtg gtacatcatt tgggatatga taagctgcat
       61 ctgcctttgg aaatgcttaa aactatagga cggccggaag gacttacact tcttggtact
      121 tcgatccatt ctgtagaaga tgcgaaagaa gcagaacgtt taggtgcaga ctatgtattt
      181 gcaggaaata tctttgagac agactgcaag aaaggacttc cgggaagagg tctggaattt
      241 cttgaaaatg tatgtaaaga agtggatatt ccggtatatg ccatcggcgg gatcacagca
      301 gaaaaaatgc cccagatcct tgggaccggg gcagcaggtg gttgtatgat gtctggattt
      361 atgcagttga aggaaaaata aaacgttgtt tttgcaacga aattaagagc tgcctgattt
      421 atagtaatcg ctgtatgttg gataactgtg tgatctgatc ttcggtcatg acggacagct
      481 ggttgatcag ttctatatga taagtaccaa gaccttttgc agcattggca cattcaccgg
      541 cacagtctaa tgtgatcgca gagaggatgc aggcagtgag agcatctgtc acagccagat
      601 aagtacctgt gatgcaggtg agcatgcagc cggtgccggt gacttttgcc atattgggag
      661 aaccattgta gatgccgtat gtggtatctt tatttgggga agcaatgata tctacttcac
      721 cggtaacaag gatcacagcg cctgtctctt ctgccagctt tctggcaata tgggccatag
      781 aatgtacggt atccggatct ttggcagtta cagcgtcagc tgcacttaca tctacgccag
      841 tgccatgaaa ggctgcgcct gcgattgcac gtatctcaga gccattgcct ttgatcacag
      901 ctggcttaca ttctttaata aaacgttttg caagttccat gcggaaaggg ctgcatgtga
      961 tccctaccag gtcgatcaca gaggctcttt ttgcattttc agcatctttc attcttttga
     1021 tggcttttcc tgccagaagc atggactcag cccttgcatc tgtaatattt ccaaggctga
     1081 cggtaagggc agctgcgctg gcagaaatgt ccgccacttc tgcaggatgt tctgccataa
     1141 tagggcgtcc tccaagggcc agaactgcgt tggcacagtc gttgaccaca atgggatgtg
     1201 tgatgcagtg gatcagcggt gcctggtcat gaagttggtc acgaacctgt tttaagcagg
     1261 aaaggagctg agctttattt atattaccat tccgattgtc catgagatga tctccttata
     1321 ataaaaacgc tgcagcagaa ccatgcaggc ctgttacagc gtttattttg caatggatat
     1381 tttttgcaca tgtatgcaca tcaggcatgt ttcttgtttg tattccagta taccactgcc
     1441 attgcaacaa aggcaaggag aacaatatat ttgatgcttc ctgcaaccgg tccaggttca
     1501 gcaacgaact gttttacggc aactgtggaa gcaagataac cgataaagcc taaaaatgca
     1561 attcccagag agtcattcac aaccgtatct gtattattgt agacctgaga gcctaaagca
     1621 gtctggaatg tggataacat atgggctttc tgtaaatgct ttaagaaaat gaaagcaagg
     1681 ctgcctccga tcagtgtacc tgcaataaag gatggcacat agaagaacca ggtaagacct
     1741 gtacgtcccc agatcagtgt cataacagga taagaaacaa tagcgccgat gatgccggtt
     1801 ccaatgactt caccgatgaa cgcacatacc agtttgcctt tggaaaggcg gtagagcata
     1861 ccggaaagaa aagcgccaaa tactgcaccg gtcagagcca gaggcgggat acccatacat
     1921 accatacgga tgatgccaat agccagagcg cacacgaaag cataccaggg accaagcata
     1981 accgcacagg ttacattaat aaaatgggcc ataggacaca ttccctctac acgaaggatc
     2041 ggggagatca cgactcccag agctaccatc atggcaagaa agacaagttt cgttacatgt
     2101 gtttttgaat ttttcatatc gtttacctct cctcattctg gctgtatgtg gaatggggtg
     2161 ttttttaata aaggccttat gaaaatggca ataaaaaaac gcatccacac ggcgcgtttg
     2221 gcaaatctct gttaaaaaat agacatagcg atggcgccgt tttacagctg taattggcgg
     2281 tcgatgctca ctagcatcct ctcaatccga aacacggagt cccatcgctt ctcttttgta
     2341 cgcagtgtca gggcgctccc cctcacaccg tctcagcccg aaagcagata caaaaggtct
     2401 aaaaacatta taaacggaga gaaaaaaatg tcaagagcaa aaccagccta tgactgaaca
     2461 gtaacctttt aaattaagat aatgtaataa aaacgtaatt gaaaatataa gttagattat
     2521 gtatgatgta ggttgtaaaa aatctgacag aatatgggag gcagaaagaa tgaaaaacta
     2581 taaaagatca ggagctgcag gtttcttttg tgcagcggct ctgtttcttg gaacgggtgt
     2641 tcttgccctg gggaccagtg catttaatgc cctggctgca gaagtttccg gacagatcac
     2701 atcctgtaag ataaccgatg ataaacagaa cgttgagatc gctttaaaca gcagtggcag
     2761 tacagaagga accgatggca aggtgtatgt gttcgagcag cctacctacc aggatgatct
     2821 gggaagccgc agcgactatc ttacttcggc aaatgcatcc ggcgccacta ccgttaccgt
     2881 tccttttaac aaaggagatg gttccgactg cctttacagc aaatttgtgc tggcagtaaa
     2941 ggaagatggt acttacaagg cagtcagcga acctcattat attaccaatc ctgagatcgt
     3001 ggcaaagaat acagaagctt ttaaggaacc tttgacaaaa aagggcttaa acattgagct
     3061 gaacatgttg gatgatgcct ttgacctggg tgtaaagtat gtgaccacca atatcgcagt
     3121 ttccagatta atgggatccg gtattgattt ccagtatgag ggcaagactt atcattttaa
     3181 caaaggcatt gtagaagatt atgataaggt catcagcgct tattccggaa aaggaatggt
     3241 tgtaaatgcc atcctcttaa atgactggag cgatacgacc tccaatctgt ttattccagg
     3301 ggtacagaaa acttccgatg catattatta catgtttaat gccaccaatg aggctggttt
     3361 tgagcagtta aaggcaattt ccgcattcct ggcagatcat tacagcggca agaatgccaa
     3421 ttacggaaaa gtttcaaact ggatcattgg aaatgagatc gaaaaccagg aatggaacta
     3481 catggggcct atggatctga ccaattatgt gaagacctac gaaaaggcat tccgtgtatg
     3541 ctacacagca attaagagta ccaatgcaaa tgacagagta tatctttccc ttagttataa
     3601 ctggatgaat gaaatggatg gccagttaaa atacggcgga aaagagatca ttgacagttt
     3661 taactccatt gccaataacc agggacagat ggaatgggga ctggcttatc atccatatcc
     3721 atgtccgctg atcgatcctg tattttggga tgacgcccag gcaacaggac tggttaaaaa
     3781 tgactttaat tcaccggtca tcaactttgc caacttaaat gtgctgactg attatttctg
     3841 ccaggaggca ttaaagacac cgtccggtca tgtgcgccac attatcctta cagaacaggg
     3901 ctttacagct tacagtccga ccaggggaga tgttccggag cttcaggcag cagcctttgc
     3961 ttattcctat tatctggtag acagcaaccc atatattgat gcttatacct taagccgtca
     4021 ggtagatgct ccttcagagg caaaagatgg actgaaatta ggcctgtggg agtgcgatat
     4081 gagcaagcca aatctgatcg aagctacaaa gaggagaaag atctggcagg tattcaggga
     4141 tattgacaaa aagaactcta ctttagaggc aagtgaattt gcaaaatccc tgatcggtat
     4201 caacaagtgg tccgatgttg tacctaactt taaatggaaa aatctggaaa agtaaataaa
     4261 ggttgatcaa aacactgcag gagaaaaatt acttctgcag tgttttgtat aaagacaaat
     4321 aatgttatat gtactggaca agcggacaaa aaaaggctaa aataagaact gactttttag
     4381 aaaaatgaag ctgctttaag cagaaataga tacaggaagg aaaatgaaaa tggggttgat
     4441 cgtacagaaa tttggcggta cctctgtagc agatacagag cgtcttcgca acgtagcaag
     4501 gatcatcact gatacgtaca aagcaggaaa tcaggtagtg gttgtgcttt ctgctcaggg
     4561 tgacactaca gatgatctga ttgaaaaagc aaaagaaatt aatccggaag gttccaaccg
     4621 ggagatggat atgctgttat ccacaggaga acagatctcc gttgcacttt gtgccatggc
     4681 tattgaggca ttggggtatc cggtggtttc tcttaccggc tggcaggcag ggatacttac
     4741 agatacggca gcaaagaatg caaggatcaa aaagatcgat acagagcgtc tggaggcaga
     4801 gctggaccag aagcggattg tgatcgttac cggtttccag ggagtagaca gaaaccagaa
     4861 catcactaca ctgggaagag gcggttctga tacttctgca gttgcactgg cagcagcact
     4921 ggaagcagac ctgtgccaga tctacacaga tgtagatggt gtatacacag cagacccaag
     4981 acatgtaaag ggcgcaagaa agctggatga agttacttat aatgagatgc tggaactggc
     5041 aactttggga gcacaggtgc ttcacaaccg ttctgtagaa ctggcaaaga aatataatgt
     5101 aaaactggaa gtattatcca gctttaccgg tcatccaggt acaaaagtga aaggggtagc
     5161 aaaacgcatg gaaaagacag cagtcagcag cgtagcaaaa gataaagata ttgcaaggat
     5221 cgcactggta ggagtgccaa atgaagtagg aacttctttc aaggtattct ctttactggc
     5281 acagaaccac attaatgtag atattatcct tcagggcatt ggccatgaag aaggaaagga
     5341 tatctgcttc acagtagcag aaggagatct taagaaggca gcagggctgt tagagtccca
     5401 caaggctgaa ctgcgttttg caagactgga gacaaacgca gatattgcca aggtttccgt
     5461 agtgggttcc ggtatgatca acaatccggg tgttgcagca aagctgtttg aggcactgta
     5521 tgatgcccac attaacatca atatgatctc taccagtgag atcaagatct ctgtattggt
     5581 agataagaag gatgcagacc gggctgttca ggcagttcat gataagtttt ttgcatttta
     5641 aataaaaata aaagcaatag agcagacaaa ccggatcaac ggttatgtct gctctttact
     5701 tttatgcaaa aaatggatac aatattaaat atgattgtgg gagcgcaaag cgcggaacaa
     5761 tccggtatca gaaaaaaata aaagtcaggg gagaaactat gtatacttgt gcaaaatgta
     5821 atgtattagc atgtggatcg caggatccgg ctgtaagaga gaaaatgcca aaaaactgtc
     5881 ctatgcggga aaaagaaaaa atggatgggg cttatgagaa atatgtctta gaggaaaatc
     5941 atgaatttta tgtaagttgt tctgagatcg aaggggaagg ctattgccaa tggccgagat
     6001 taaaagaaac agtggaattt tgtaaaaaaa tgggttataa aaaaatagga ctggcatttt
     6061 gcaggggact gcgaaaagag gcgcgtgttg tggcagatct ttttacagcc cagggctttg
     6121 atgtggtatc tgtgatctgt aagaccggtg gttttccaaa ggagtcggta ggaattccga
     6181 aagaaaagaa ggtcaatgca gatgcatttg aaccaatgtg caacccaatt gcccaggccg
     6241 aatttttaaa ttcccaggaa acagagttta acattgcgct gggactttgc gtaggacatg
     6301 actctatgtt ttacaaatat tccaaacaaa tggtgactac actggtggca aaggaccgtg
     6361 tgctggcaca caatccatgc ggtgcgatct actgtgcgga aggatatttt aagaagagat
     6421 tacagtagca gtttatgcca gaatatggtt ttggtggatc tggatcttgg agacttgaaa
     6481 agtgaggaca gactaaatgg aatatattgg aaataagaaa aagcagggca gtattgtgga
     6541 acttctgcgg gatgagatat tggcgggaaa tatacctgat ggcagtgaga tgacacagaa
     6601 tgagctggca gaaagtctgg gagtatcccg gatgccggta agagaagctc tgatcttgtt
     6661 ggagtatcag ggattgatcg atcgtctgcc taataatcat gtgcgggtgg catctctcac
     6721 agaagactac ttccgccata ttttaaaaac aggctgtaga ttagaacttg aatttttaca
     6781 gaatttagag gaagaagaac gaagcagtct tcctgatagg gaattgaatt ttcacaggga
     6841 gatctggcgc aactaccctt acagtctttc cagaaaaaat ctggagacga taacggagat
     6901 ctacctttat tttgttatat ccagacggaa cccggaaggt gtctggggaa atgtccggga
     6961 gaaaacagag gaagagatct tattaagtaa gagtatagaa aactggaagg ctggaaaaac
     7021 agaagaagga gccggacagt tagaggaata ttttgaaaaa atggcagtga aactggctgc
     7081 tatccataag gggagggatt aaagagttat ggttacatta aaaccaatta aattgctgcc
     7141 tgcaagggaa cgggtggctt ctgcactgcg aaaagccatt atctcaaagc agatcggtga
     7201 aggagaagtt attacactgg aagcaactgc ccagcagtta ggtgtatccg ttacaccggt
     7261 gcgtgaggca tttcagatac tggcaagaga tggcctcatt gacttaaaac agaacaaggg
     7321 cgcaactgtc cttggtttaa atgaaacaac cataagagag cactatgaaa taagagcggc
     7381 gcttgaaagt gcggcctgtg tgctctgctg tcagagaaaa gcagatcttt ctgctataag
     7441 aaactgcctg gaaatggcag atggggcact ggagaaaaaa gattccagca gttattctaa
     7501 ctataaccag tccttccact atgagatctg gacagcagca ggaaatgaaa agttaaaaac
     7561 aatgctgtct gaaatgtgga acggtctttc tatgggctta aagtccacag aagaagaata
     7621 cgccagaaat tcccaggaag agcaccgaca gatcctggca gcccttgaaa gcggggaccc
     7681 gaagcaggcg ggggagaaga tggtatttca tattatccga agcatgaatg atgtactgac
     7741 aagatactag taaaaagata tgacttcccc gctggcagcg gggatttgtt atatatgcac
     7801 aataaaatat caaaaagaat atgcagaacg ctgaattggt aaattactat tgacaattac
     7861 agcgttctgt tctattataa aaacatgaaa tggatacatt attaaatata aaatatcaaa
     7921 tataagatat aaaataaaaa taatggtcca ggacagcagc tgcaaaacat aagtgttctg
     7981 actggcaggc gggacaccgg ttacaaagta ccttaaaaaa tgaataaggt ttatacagtt
     8041 atataaaacc gaagggggag tttatgagtc catcaactat aaccatcctg tttctcttgt
     8101 ttgcaattgt aatgtttgta atggagaaga tcccgctggg ggtaacatcc atgatcgttt
     8161 gtgtaggtct ggttgttact ggggttctgg atgcgaagac ggcatttgcc ggttttattg
     8221 acagcaacgt aattctcttt gtggcaatgt ttattgtagg cggtgcattt tttgaaacgg
     8281 gtatggccaa caagatcggc ggtattgtta caaaatttgc caaatccgaa aggatgctga
     8341 tcgcagcagt tatgatcatc gtaggtgtga tgagcggcgt tctttcaaac actggtacag
     8401 cagcagtact gatcccggtt gtgatcggta ttgcagcaaa atccggttac tcccgttcca
     8461 gactgctgat gcctttggta tttgcggcag ctatgggcgg caacctttca ctgatcggag
     8521 cgccgggcaa tatgatcgcc cagagtgctt tatcagagat cggacttggc tttggatttt
     8581 ttgaatatgc cattgtagga cttccgatcc tgatctgtgg aacacttttt tataccacac
     8641 ttggtatgaa gcttttaccg gacaggcagc caaaagatga cggggcgttt gataccacag
     8701 ctgattacag caatgtaccg gcctggaagc agaagctttc actggtgatc ctgttgctta
     8761 ccttactggc tatgatcttt gaaaagaaga tcggcatcaa gctgtatgtt tccggatgta
     8821 tgggagctct ggcactgatc attaccggtg ttatctctga gaaacaggca ttaaattcca
     8881 ttgatttaaa gaccattttc ttattcggtg gaaccctttc actggcagca gcccttgaaa
     8941 aaacaggtgc cggtgagatg gtagcagaga aggtaatgca cctgttaggt gaaaatcctt
     9001 caccttatgt tttaactttt gcagtattta tgctttgctg tattatgaca aactttatgt
     9061 caaatacagc aactacagca cttttagttc ccatctgtgt ttccattgca cagggaatgg
     9121 gagcagatcc aagagccgta cttatggcct gcgttatcgg tggctcctgt gcttacgcaa
     9181 cacccatcgg aatgccggca aacaccatgg tacttggagc aggcggttac actttcttag
     9241 actatacaaa gtcaggactg ccgcttatcg tgattgcgac catagtcagc atgatcattt
     9301 taccgattgc atttccattc ttcccataag ttgttttatg ggaacatgaa ataaaagcaa
     9361 taaaacaaac attacatatt attaagacaa ttcaaaacat attataagga ggcttaaaca
     9421 tgagcagaac agaacaggta gaggagctta ccaattatat ggcgcacttt gttgcccata
     9481 tcggtaagaa actgccagat gacattattg ccaagttaga tgaactggca gaaaaggaag
     9541 acagcccatt atcaaaagta atttatcaga ccatgaagcg caatcaggat ctggcagtaa
     9601 aattaaaccg tccttcctgt caggatacag gtgtccttca gttctgggta aagtgcggaa
     9661 ccaaattccc actgatcgat gatctggaag tactgttaaa agaggctgtt gtaagagcaa
     9721 cagtagacgc gcctcttcgc cacaacagtg tagagacttt tgatgagtac aacacaggca
     9781 agaacgttgg caagggaact cctactgtat tctgggatat tgttccaaac agcgacaaat
     9841 gtgagatcta cgcatacatg gcaggcggcg gctgcaccct tcctggaaaa gctatggtat
     9901 taatgccagg cgcaggatac gaaggtgtta cccgtttcgt actggatgtt atgacctcct
     9961 atggtttaaa tgcatgtcct ccactgttag ttggtgttgg tgttgcaact tccgtagaga
    10021 cagcagctct tctttccaag aaagccctca tgcgtccttt aggaagccac aatgaaaatg
    10081 agagagctgc aagtatggaa aaacttctgg aagacggaat taacgagatc ggccttggac
    10141 cgcagggtat gggcggaaag tactctgtta tgggcgtaca tattgaaaat acggcaagac
    10201 atccatctac cattggcgta gcagtaaacg taggatgctg gtcccatcgc cgcggccaca
    10261 ttattttcga taaggacctt aactatacaa ttacaactca ttcgggggtg actttataat
    10321 gttagagatc aaagatggaa agaaaatttt aacaacacca atcagtgacg aagatttaaa
    10381 agatatccat atcggtgaca ttatttactt aaatggtaac ttaacaacct gccgtgacgt
    10441 agctcaccgc cgtctggtag aagaacacag accactgcct gtagatgtaa gaaacaatgc
    10501 cattttccac gcaggcccta tcatccgtcc tttaggagat gacaagtttg aaatggtttc
    10561 cgtaggaccg actaccagta tgcgtatgga aaagtttgag aaggaattcg tagagcagac
    10621 cggcgtaaaa gtgatcgtag gaaaaggcgg tatgggacca aacaccgagt acgcatgcaa
    10681 gaactttaaa gccatccact gtgtattccc tgccggaaac gcagtagtag cagcaactga
    10741 agtagaagag atcgtccgtg cagagtggcg tgacctggga atgcctgaga ccttatggaa
    10801 ctgccgtgtt aaggaattcg gaccactgat cgtatccatt gatacagagg gaagaaacct
    10861 gtttgaggaa aataaggttg tatttaatga acggaaagaa aaagtttacg aagagatctg
    10921 caagcatgta agctttatca agtaatgtaa tgagtgcgca ataaaaacct gccatctctc
    10981 ctgcggcgta gtgaaaacga ctcgcggcag gagcggtggc attttttgcc ttaaaatttg
    11041 ttaataaacc atttttaata aaaggaaaca cttgccattt gacaaaaata tcactataat
    11101 ataaacggca atatttctgt attttataag aaagcgctat ttttatgtgc tttccgctaa
    11161 atactgtaaa cttaagaatg atggaggact ggaaaatgag taattcagca caaacttcag
    11221 caagcagccg tattgcagcc ctgcttgatg agaacagttt tgttgaagtt ggagcctata
    11281 ttgcggctag aaatactgac tttaacatga ctgagcagga aacaccggct gacggtgtag
    11341 ttaccggtta cggtaccatc ggcggatgcc tggtatatgt atacagccag gatgctgctg
    11401 tactgggcgg ttccatgggt gagatgcatg caaagaagat ctgcaacatc tactctatgg
    11461 caatgaagat gggagcacct gttattggtc tggtagactg tgcaggactg cgtcttcagg
    11521 aagcaactga cgcactggat ggttttggaa agctgtattt aagccagaca atggcatctg
    11581 gagtaattcc tcagattatg gcaattttcg gtacctgcgg cggcggtatg gctgtatctg
    11641 caggtatggc agatttcacc ttcatggaag atacttctgc aaaactgttt gttaactccc
    11701 caaatgccct ggagggcaat tacaaggcaa aatgtgatac ttcttccgct aagttccagg
    11761 cagaagagtc tggtctggtt gatttcacag gtgatgaagc atctgtatta tcacagatcc
    11821 gtacactggt atctatcctg ccttctaaca atgaagaaga tctgtctgaa gttgactgca
    11881 cagacgattt aaaccgtatg tgtgctggta tcgaaggata taccggagat actgctgcag
    11941 ctttacagat gatctctgat aataacttct ttatggaagt taagaagaac tacggcgctt
    12001 ctatggtaac cggatttatc cgcttaaacg gaagcacagt aggatgtgta gctaaccgca
    12061 gcgaagttta cgaaaacggt gagaaggtac agaccttaga ggcagctctt tgcggaaaag
    12121 gctgtgagaa ggcaactgat ttcatcaatt tctgtgatgc attcagcatt cctgttctga
    12181 ctttagtaaa tgtaaacggc taccgtgcaa ctatgtgcag cgaaagaaag atcgctaaat
    12241 tagcagctaa gctgacctac gcatacgctg atgcaactgt accgaaggta actgtcgttg
    12301 ttaagaatgc actgggaagt gcaggtctta ccatgggcag caagagcctg ggcgcagacg
    12361 ttgtttatgc ttggccaaat gcagtgatcg gaactatgga tccggcagaa gctgttaaga
    12421 tcatgtatgc aaaagagatc gaagctgcag atgacgcagt tgcaatgatc aaagagaaaa
    12481 ccgctgaata taccgctatg cagtccggcg ctgttgctgc tgcaaagaga ggttatgtag
    12541 atgacatcat caaggcagaa gagacccgtc agagagtgat cgccgctttt gagatgctgt
    12601 acacaaaaag tgaggaccgt ccttccaaga agcatggcac ggtttaaaga gaggtgacat
    12661 gtatatgaga cgatttgtaa agagaatggc agcagcagtt cttctgtccg cctgcctggt
    12721 aagcctttct ggctgctcct ctaaccagga tacagcagag actgattcca ccatcagcat
    12781 ggacggaact cctgtagatg attcaatggc tcagtccatt atgctgtctg cagcacagac
    12841 tttaggcgta ccaaaggatc agctgatcgt tcagaaaact ctggcagagt caacaggcga
    12901 tacgactaca gcagctattt atgaggctca gctggaagtt cgcgaggata tgggcgaatt
    12961 aaagaatgta aacatggatg aaggttctgt tgttcttctg gcagatggca gctatacggt
    13021 tgtgatcccg gttgacttta cagagggaac caagaaatat gtgatgaaca tcaatatggc
    13081 tacccagcag atccaggctg agtttacaga tatgtccgca ggcgttgtag aagatacttc
    13141 tatgggtgct ctgttaaaga cagctactgt atacaccatt attggtatcg gcactgtatt
    13201 ccttgtttta attttcatca gtatcctgat ctcatgcttc aaatacatcc atgcatggga
    13261 agaaagcaag aagaaagcag cagcacctgc tcctacacca aaggcagcag cacctgctgt
    13321 taagccagta gcagcagcac cggcagtaac cggtcctgat ctttctgatg atgcagaatt
    13381 agtagctgtt atgactgctg ctattgcagc atatgaaggc agcgctacat ctaacggtct
    13441 ggtagtacgt tctatccgcc gtgtaggtct gggaagataa tcactgcaca ttcaggttag
    13501 aaaaccagtg tgcttataca acaagtagga ggaaacaaac tatgaagaca tataccatta
    13561 cagtaaatgg aaaagcatat gcagtaaccg ttgaagaagg cgcagcagca gctggcgcac
    13621 cagtagcagc agctccagtt gcagctccag cagcacctgc accagcagca gctccagctc
    13681 cggcagcagc accagcaggc gcagcaggtt ccgttcaggt taccgctcca atgccaggta
    13741 aggtagtagc agttaaggct tctgtaggac aggctgtaaa gaagggcgaa gttgtcctgg
    13801 ttctggaagc tatgaagatg gagaacgata tcgttgctcc agaagatggt acaattgcca
    13861 gcattaatgt agcaaacggc gatgcagttg agtccggcgc tgttttagct actctgaact
    13921 agtcaacaag atggatccct taatttaagg attccattat ggaggtaaca ttatggattt
    13981 tggcaatttg gtatcaaacc tgcttggcca gatggcgttt ttccatctgt cagcaggaaa
    14041 ctacattatg atcggtgtag cgctggtctt cttatacctg gctattgcta agggatttga
    14101 gccgctgctg ttaattccga tcgcatttgg tatgctgctt gtaaatatct atccggacat
    14161 catgtattct ccggaacaga cttcaaacgg cgtaggcggt ctgctgtatt acttcttcac
    14221 tctggatgag tggagtatcc ttccgtccct gatcttcatg ggcgtaggtg cgatgacaga
    14281 cttcggtccg ctgatcgcaa acccgatcag tttcctgatg ggtgcagcag ctcagttagg
    14341 tatttactct gcatatttct ttgcaatctt attaggattt tcaggaaaag aagcagcttg
    14401 tatctccatt atcggtggtg cagacggccc tacttccctg ttcctttgca gtaagttagg
    14461 acagacccag atcatgggac cgatcgctgt tgcagcttat tcttatatgg ctctggttcc
    14521 gatcatccag ccgccattta tgaagcttct tactactgat aaggagcgta agatcaaaat
    14581 gtcccagctt cgttctgtat ccaagctgga gaagatctta ttcccgatca tcgttactat
    14641 cgttgtgtgc ctgatcctgc catctactgc tccgttagta ggtatgctga tgttaggtaa
    14701 cctgttccgt gagtgcggtg ttgtaaaaca gcttcaggag actgcttcta atgcaatgat
    14761 gtacatcgtt gttatcctgc ttggtacttc tgtaggtgca accaccagtg cagaggcatt
    14821 cttaaatgca actactatca agatcgttat tttaggtctg gtagcattct gtgtgggaac
    14881 catcggaggc gttctcttag gaaagatctt atgcaaggta actggcggaa agatcaaccc
    14941 actgatcggt tctgcaggtg tatccgcagt tcctatggca gcccgtgtat cccagaaggt
    15001 tggtgctgaa tatgatccta ccaacttcct gctgatgcat gcaatgggac caaacgttgc
    15061 aggtgttatc ggtactgcag tagcagccgg tacctttatg gcaattttcg gtgtataaaa
    15121 accaacattt actgcacccg gtgctgtttg cagaccgggt gcctataagg aggatttaaa
    15181 ctatggcaga gatagagaag aagccagtaa agatcgtgga aaccgtcctt cgtgacgctc
    15241 atcagtcttt acttgctacc agaatgacta cagagcagat gctgccgatc gtagacaaga
    15301 tggataaagt tggctaccac gcagtagagt gctggggagg cgctacattt gatgcctgcc
    15361 tgcgtttctt aaaagaagat ccatgggagc gtttaagaaa gttaagagac ggctttaaga
    15421 atacaaagct gcagatgctg ttccgtggac agaatattct gggatacaac cactacgcag
    15481 atgatgtagt tgagtatttc gtgcagaagt ctattgcaaa tggtattgat atcattcgta
    15541 ttttcgactg cttaaacgat atccgtaacc tggagactgc tgtaaaggca acaaagaagg
    15601 aaaaaggaca tgctcagatc gcactgtgct acaccttagg tgatgcttat actctggatt
    15661 actggagaga cattgctaag agaattgaag acatgggagc tgattccctg tgtatcaagg
    15721 atatggcagg tctgttgact ccatacaagg ctactgagtt agttaaggcc ctgaaggaag
    15781 gaaccacact tccgatcgac ctgcacaccc actatacttc cggtgttgct tccatgacct
    15841 atatgaaggc tgtagaagca ggctgtgata ttatcgactg tgcaatgtct ccactggcac
    15901 ttggtaccag ccagccagca actgaagtta tggtagagac cttccgcggt actccatacg
    15961 atacaggata cgatcagaag cttttaggcg agatcgctgc tcacttccag ccgatccgcg
    16021 aggaagcatt aaagaccggt ctgttaaatc caaaggtatt aggcgtaaat atcaagactc
    16081 tgcagtatca ggtacctggc ggtatgcttt ccaacctggt tagccagtta aaggaagctg
    16141 gacaggaaga caagtatcag caggttctgg aggagatccc gagagtacgt aaagactttg
    16201 gtgagccacc tctggttaca ccttcttccc agatcgttgg tacccaggca gttcttaacg
    16261 tattaatggg cgagcgctac aagatggttc caaaggaatc caagaagatc atgcttggtg
    16321 aatttggcca gactgttaag ccatttaatc cagaagtaca gaaaaagatc atcggtgatg
    16381 agactccaat tacctgcaga cctgcagatt taattgcacc tcagatgcct cagttcgaga
    16441 aggaatgcgc tcagtggaag cagcaggatg aggacgtatt aagctacgcc ctgttcccaa
    16501 atgttgccaa ggagttcttc cagtacagag aagctcagca gaagaaagta gatgctactg
    16561 tagctgataa agagaataag gcttatccag tgtaattttg gtatatccgg aataagataa
    16621 gtatggaacg cgtccctttg tggggcgcgt tttttgtgtt agttttttgc cgctgcatct
    16681 ggcgtaaatc tgttggagat ggtacactga tataccagaa gatgttttgt ccaatgagga
    16741 gggggaacat gctgaaagta ttgatcgcag atgatgaaga aaagatctgt cagctgatca
    16801 taaagctgat caactgggaa gaaatggggt taaaaatagc ggcaactgct tctaatggta
    16861 ttgaggcatt agagcaggca aaaatctgta aaccggagat tctgatcaca gacatacgga
    16921 tgcctggaat tgatgggatg gaactgatta ggaaagtaaa agaaaaactg ccggatacag
    16981 agatcatcat aatcagtgga tacaggcatt ttgaatatgc ccagacggcg atccgttatg
    17041 gtgtgagaaa ttatctctta aaacctatta aaaaagagga attacgggat accttacaaa
    17101 agatcgcaga tatttacagg gagaaaaatg aacagttaaa ttttgaagaa agagtccgcc
    17161 ttgccttaaa aaatgatgca ggaaagctgc gtaccagttt tatttcccgt atgatctacg
    17221 gagaccggga gcgggaaaat attatcagcc tgaaagaact gaacaatcag tatcatttcc
    17281 atttccgtga gggagtgttc caggttgcag ccatcaaatt tgaccatgta aaccacaatg
    17341 acagctgtat ttccttcctg gcagacaagg cagcaggcct ggcgttacag tatctggaac
    17401 cggtatgttt tgaccatgag gtttatgcag agttttctac cttttatctt ctgctgaatt
    17461 tcggggaaga agagagtaaa aatgtgcgga aaaatgtgcg ccagatgctg gatgaactga
    17521 aagcccagga aagtatctta aatggaatga gtgttaccat aggaatggga agcatggtaa
    17581 acacagcggg cagtatcaag aagtcttttc tggacgctgt atttatgata aagcagaggc
    17641 ttcttttagg aaccgggcgg atgctggaaa tggatgaagc acaacagaga aaaaactctg
    17701 attttataaa cagtgaggca ttttaccgtt ttaacaggga aatggaacgg gcagtcgaag
    17761 ctttaaatgt agtggagaca aggcggacta ttgaaggtct gcgggatgta ttaaagaact
    17821 atccggggat caccggttat gagatcctgc agatgactaa ggaggcctgc aatcattatt
    17881 tgttttgcat gaagagcagg gggatccgtg tggacagtga agctaaattt gtggaagaat
    17941 tcagtaaaga cgcaggggaa tgtgcagata tagaagaact gttccgcctt ttaggtggga
    18001 cgataggaag atctctgcaa aaagcttctg aagcaaagaa ggcagaagat aaccggccca
    18061 tacggtaggc aaagcagtac atagaagaaa attacagccg ctcgcttacc ttagaagaag
    18121 taagtgagat ggctggattt gctccgggat atttcagcac gctttttaag aaagaaacag
    18181 gggtaacttt tctggaattt ttgcagtcag tgcgtatgga tgcagcgaaa aaactgctgg
    18241 tttccggaaa tgagggaatg aatgtgatct gcgaaaaggt aggctatgca gatgtgaaat
    18301 atttcacgaa atgttttata aaatataccg gactgaagcc gggagagtac aggaagatct
    18361 attcatgaat aaagaaagtt atctttttga ccgtatacgt tggggcgcag cgattttttt
    18421 gatttttcat atcagtctgt tgtttatcct gttccttcag gattggaaga cggagaaaat
    18481 gaagtggttt attctgtttt taggagtgct ttatgttatc ctgctttatg ccgcttatca
    18541 gctgatctac cggcctttaa aagtgaacag ccggataaag gcggcttttg ccagagggga
    18601 cagttatgag gatatttacc atatgcctta tcttttaaat gaggaagagc agaatatgct
    18661 gcgtcgtttt tatgccctgt tagacaaaag cgagctgatc actgtatcta agaaaaaatc
    18721 ggaatatctg gcattacaga atcagatcaa tccccatttt ctttataata ctctggaagg
    18781 catccgggca gaggcgctga tcgcaggggt ggattccatt gcggagatga cggaggcact
    18841 ggcaacttac ttccgttata ccatttccca ggtaaataac ctggtcacat tggaagaaga
    18901 actggcaaat gtggaaaatt attattatat ccaacagttt cgttttggca gcaagctgga
    18961 tctgaggatc cgctatgact gtgaggatga agaggaagcg cttatgtgcc agcttcccaa
    19021 gctgactttg cagccgatcg tggaaaattc catttatcat ggactggagc gcaagattgg
    19081 aacaggtcat ctggatatta aggtcactgt gacagacagc catctgatca ttatgatctc
    19141 agatgatggt gtaggcattg aaaaaggtca ggtgaaggtt atgaatgaac agctgagagc
    19201 cctgagactg gaggaggaaa acaacgggga aaccggtaaa aaaggcggaa ttgcagttaa
    19261 aaatgtaaat aaccgtatta agcttttgtt tggggaagaa tatggcattt atatctacag
    19321 ccagccggat gtgggaacgg atgtggagat cacacttccg gttgtaagga attgatgtaa
    19381 cgaacatgct gataaacagg aggcagctat gaaacaggaa atacttcgtt ttgaaaatgt
    19441 gacctgcaaa aaagacgggg ctgtttattt ggataatttc agtttttata tgactgcagg
    19501 tgagatcgta gggttgcttt ctgtcaatga ccgggggatc aaagaactga tcgaactgat
    19561 ggcaaagaac catcctattg atgcaggaag agtgtatatg ggtggcaaac tggtaaacag
    19621 ttatttaaat caggaggaaa catctaaccg tatttaccag atcgaccaga aaagctctct
    19681 gatcaatgat ctgtctataa cggataacct gtttgttatg cgcccaggat ttaagaaaca
    19741 ggtgatcaat gaatgggttt taagagaaca ggcagacaga gtcttaaaac agctttccat
    19801 ggatctggat ctgaataaaa gggtggaaag gcttactacc atggagcgga tcatggtgga
    19861 gatcgccaag gcctatttaa tgggaagcaa gcttttagtg gtgctgtatc cggaaaaact
    19921 gatcggtcag gcagatttcc cgcagtttca ccagctttta agaaaaatgc agagcatggg
    19981 gatctctacg ctgtatttct gctatcatca ctggatcatg tttaagatct gcgacaggat
    20041 cgccctgttt tccagaggaa ggataaaaaa attatttgaa ccagaggaat tcagggaaga
    20101 ggctatagct ccctatattt attattttaa agaaaataca gtatttaagc cgaaagtgga
    20161 tcggggatat atgctggagc taaaaaacat tacagatggg gacatacata acctgagtct
    20221 gggaattaag cctggggaat gcattacgct tttggattca ggtgaccaga tcatggaccg
    20281 gctgaccggg atccttgcag gggaatacgt ggactatgag ggagatattt tctgctcaca
    20341 caaaaaaatg gaaaaaaaag taaaaagcag cctggaccgg ggaatgatcg ttttagatga
    20401 taatccagcg cagtctttcc ttttttcgga aatgtcctat ctggaaaacc ttaccttttt
    20461 actggaccgt aaattaaaga aaagcatttt aaagcagtca tatattaaca gtgtgcgcag
    20521 tgaatattat ggggaagcag gagatgttat tgacacaaaa tatattgatt ccctttcttt
    20581 aaaggaaaaa tatgggctga tctataataa aataagcctg ttccatccta agatcctggt
    20641 ggtaaaaaag ccttttgctt atggggttag gcaccatgtg ggcgctctgg ggccagggca
    20701 ttggaaaga
//
LOCUS       DBMDUA010000025        20653 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1599_length_20653_cov_3.621837, whole genome shotgun sequence.
ACCESSION   DBMDUA010000025 DBMDUA010000000
VERSION     DBMDUA010000025.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 20653)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20653)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20653
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1599_length_20653_cov_3.621837"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="ACZPCL_03535"
     CDS             complement(<1..361)
                     /locus_tag="ACZPCL_03535"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR1161593.1"
                     /translation="MMELPVTRMSRIVMPASTNDAGNLYGGRLLDWMDEVSGIAAKRF
                     AHSEVMTVGIESIRFMRPMPQGSFIDINAEVVHVGNTSMKIKITVMMDQDPEEEQVQT
                     AEAFFTFVAVDENGKPHK"
     gene            complement(392..1480)
                     /locus_tag="ACZPCL_03540"
     CDS             complement(392..1480)
                     /locus_tag="ACZPCL_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012407.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR1161594.1"
                     /translation="MKLEAAVIQGDGVGPEMMEEAVRVLQAVCRRFGHHLKLYPVLAC
                     SQAIEAGKEPLPQESLKICTQVPAVLFGNSGLKKYQDWPLDKRPEGALMILRKVLQVS
                     TNIRPVRMYPSLKCFSPLKAERLEQGMDVAFVRDIAGGVFCSAKVQGNGDGGREAYEY
                     EYYNETIVRKTAYTAFKLAKSRKNKVTNLDKSNVLGSSRLWRQTVQQVSEDFPDVELE
                     HLYIDNAAMELIRNPGRFDVFVTSNLFGDIISDEGTELTGTPYLYPSAELSNTEQGIY
                     TPNQLHYPDESVIGKDMVNPIGMIAAAAQMLRLSFGLEAEAGAIEEAIRQVLAANIAT
                     ADIWHKGKILVGTKEMGKRIADEIEAVS"
     gene            complement(1517..2173)
                     /locus_tag="ACZPCL_03545"
     CDS             complement(1517..2173)
                     /locus_tag="ACZPCL_03545"
                     /inference="COORDINATES: protein motif:HMM:NF040019.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczE/YyaS/YitT family protein"
                     /protein_id="HIR1161595.1"
                     /translation="MLMVKKALIYVTGLLFMAFGVAFSVNSSLGVSPVNSLPYVISRI
                     TGLDLGNCVIGIFAFYILVQILLLRKKFRPIDLTQLIFSTIFGRFVDVAKKVVGDFAI
                     PTYPGQLVMLAISIVFVAIGVCLYMDVQLVNMPMEGMTHAVNRVFFPDKPFHDIKVIM
                     DCLTVVIGIVLSFVFLGKLDGIREGTVICALLVGKLMKPMQKVLKPAVEKICGIKGSR
                     "
     gene            complement(2167..3270)
                     /gene="buk"
                     /locus_tag="ACZPCL_03550"
     CDS             complement(2167..3270)
                     /gene="buk"
                     /locus_tag="ACZPCL_03550"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HIR1161596.1"
                     /translation="MKNYKVFAINPGSTSTKIAMFEGEKEIFSRNISHDAEKLKEFKE
                     ISDQLPYRKEMILDVLKEEGQTLEGVDAFSARGGGLVNVEGGVFAIGDKLLEHARTCY
                     TVKHPATLGAQLADAFSKEFGGAAFVVNPPDVDEFQDVARVTGLKGVYRESRIHALNQ
                     KEIGIRFAAKQGKKYEDMNLIICHIGGGISVTAHRMGKMVDSNDIANGDGPMAPTRCG
                     QLPVKDVVTMCYSGKYTEKEMREKITKTGGLVDHLETSDAREVTAMIENGNKYAKLIY
                     DSMIYEIGKTAGSMAAVLHGKVDGIVLTGGISHDKYVVEKLTEMLSFIAPITVMAGEF
                     EMEALAAGAIRVLSGEEEAKTYTGIPVWSGFTC"
     gene            complement(3372..4715)
                     /locus_tag="ACZPCL_03555"
     CDS             complement(3372..4715)
                     /locus_tag="ACZPCL_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015355.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HIR1161597.1"
                     /translation="MEKKLNFKVCGLPAKYFIPFFIIVMATVYLGFMPVVKIYSNDAG
                     KYMATSFIMTIAYLMAVGGLFFWLGNTIPIVNNYLGGACLLPLIGASFLNYVGLVPQE
                     LVNGVKVLMGGGFQDAYIAMLLVGSILVMDRKVLLGATARYMPTILGSQVFALGFCML
                     AGLVTGYGIPEALFDIGAPCMSGGSGGAMTTLPALYSSLSGTDMTPMAGKFLCYASIA
                     NVLAVVLAAIAGSVTAKMKGLNGNGEILAAKSAQVQKEEAQEQKRPGTSADYVALGSG
                     IFMSFCLYLLGNILGHIPGFSVIPGLAWTIILGIIIKFTGILPDQFADNCVYSMNFAL
                     KALLPMLIAGIGINSLKFSTLAEYFSPGAFVVIFLAVFGAMLGAMIFGKLSGLWAYEA
                     GVTAGLCCCNIGGSGDLAVLSAANRMNLLAFASISTRIGGALMVIWIGLLYPLFH"
     gene            complement(4730..5623)
                     /locus_tag="ACZPCL_03560"
     CDS             complement(4730..5623)
                     /locus_tag="ACZPCL_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013665.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HIR1161598.1"
                     /translation="MIKNFEQLKEMLKAMPVKRKVAVVPAQDEHTLEAISHAYRDGMV
                     EPVLIGDEPKIREILAQIGTDADKMTIIHVEDPTEAIQKAADMARDGEVDCIMKGKTE
                     TGALMKVLVNRERGIRKNDTMSLLAFMESPNYHKVFAITDVGLLTYPSKDQKKAAIQN
                     AVEAFHALGVEQPKVAILAAVEKVNPKMKETVEAAEIKEEGVEGCIIEGPISYDLAMD
                     PASAPIKGYVSPVAGDADLLVVPDIVSGNIAAKTITVIGGGRTGGVVLGAKVPVLLVS
                     RAANADDKYMSIVIAALVGSR"
     gene            complement(5702..7510)
                     /locus_tag="ACZPCL_03565"
     CDS             complement(5702..7510)
                     /locus_tag="ACZPCL_03565"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HIR1161599.1"
                     /translation="MEKKFSGKIWKFGNDIDTDTIIPGKRGTIPDRNEMKKYAFELLK
                     PEFGSTVQPGDILVAGTNFGCGSSREQAATVLSYNGVRCIIAKSFARIFFRNAFNSGI
                     LLLTCDQIQDVCEGGDIVTVDVDAQTVSVNGKTFKVGAVPENLYNIVANGGLIEDTKK
                     RLAAGNVKMDIKPLSMEQCRKKGYTMVEKILKKNAGKEHVAPGDIVITKPDMFMIHDI
                     YTTYLLETMKDIGADKIDDPDKVTIVWDHCMPTAVAKNDYDHYEAGLELAKTYGIKKL
                     HIGEGICHTIMHEAKYAKPGEIATATDSHTTTYGGAGNFCSGIGTAEMAAALITGELW
                     FKVPEAIKIVLNGHLRDGVMSKDVILRILGDIKADGGQYKSLEFTGPAAHEMSMEQRF
                     TVANMALEAGAKCGLFEADEKTAEYYGMPLEDIDWVCVDDEAKYEKVLTYDVSELEPQ
                     LSCPQGVDNVHPISEVKGTKMDEVYLGSCTNGSIEDMEAAAKILKGRKVAKGLRFIVV
                     PATNTIFKQAIERGYIKTFIEAGAVICHPCCGLCCGMPYGLMTDDERILLTANRNFIG
                     RQGTKKTLGYLSSPTVAAATAVMGVVTDPADLPALA"
     gene            complement(7521..8642)
                     /locus_tag="ACZPCL_03570"
     CDS             complement(7521..8642)
                     /locus_tag="ACZPCL_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012550.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR1161600.1"
                     /translation="MSTFVERLLGGKAGTVVTVEPDWIVINDGVSHAAVEEISAVAKP
                     EKVMVIYDHDVPTGRPEAAAILRKNLAFAEKYGCPYIQAEGVGYQYMLNEVVKPGQII
                     VGGGSHGSIFGSIGALGINVSIPELARAAETDRYSIIVPETVYVNLEGSLKEGVTVMD
                     AALAFLAEDHESNRKAVEVYAPSFDAHEKAVFCSMACITGAFTASITEEKQSAGLTLN
                     LATVEPMVMLPCGDRNDQKKAGIASRASKAGMELNAGQIGGYTGGTIEELRKAASMLD
                     GHKLALGFRLSICPATSRDYLQAAEEGILTKFIDFGAQINAAGDHSVVIQGPGAMGHK
                     ERLVTTGLYTFTGSMGCDDAEVYTGSVETVMNASFAKQM"
     gene            complement(8660..12223)
                     /gene="nifJ"
                     /locus_tag="ACZPCL_03575"
     CDS             complement(8660..12223)
                     /gene="nifJ"
                     /locus_tag="ACZPCL_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR1161601.1"
                     /translation="MSKVSRAMDGNEAAAYASYAFTEVAAIYPITPSSPMAELVDKWS
                     ANGMKNMFGQQVKLVEMQSECGAVSAVHGALDGGVLATSYTASQGLMLMIPTMYRIAG
                     QLKPGVIHVASRNVATHAISINAEHSDVMACRQTGYAMLASSSPQEAMDLGAVAHLAA
                     IKGHVPFLHFFDGFRTSHEIQKVECLDYEDLKKLVDWKELEKFRENALNPEHPVLRTT
                     GQYSDTYFQSREACNTYYDALPDIVADYMNEISKITGRDYKPFNYYGAPDAERVIVVM
                     GSASGVLCETVDYLNAKGEKVGFIDAHLYRPFSAKYFLSQLPETVKMITVGDRTKEPG
                     AAGEPLYEDVCSVILESGRNIKVLACRYGLASKDYTPDQVIAQFDNMKAAEPKNHYTV
                     GINDDVTHLSLPVGPEVNTVPEGTISCKFWGLGSDGTVGANKNTIKIIGDHTDLNVQA
                     YFEYDGKKSGGVTRSHLRFGKKPINSTYLVNHADFIACHNKSYLDRYDILADAKEGAN
                     VLITCDWKGEELEKHLTPAFKKAAALKKIHLYTIDSTAIAAELGLGSRTNTILQAAFF
                     KITGIIPIEEAVGYMKKAILKSYGRKGEKIVNMNYAAVDKGVEMVEEAEVPARWADIE
                     IPEEKIDENLPKWIRKIQMPVNAMKGDNIPVSDFTDLPDGTMPQGTSKYEKRGIAVNI
                     PVWDSEKCIQCNMCSYVCPHSCIRPFLVNEDEAAKAPETFTTTAVKGKGAEGLRFRLQ
                     VSALDCTGCGSCANVCPSKEKAITMKPLDNNLNEADNWEYAMKLAPKANPFGTTTVKG
                     SQFEQPLLEFPGSCPGCGETPYAKLITQLFGDRMYVATATGCSQVWSTSFPSFPYTTN
                     HKGQGPAVGGSLFENNAEYGMGIWLSVEQQREKLAKHVEELAEKTDDAKIKETAAAWL
                     ASYNDKSSRETGEALKNAILESGLLSENADLVAANVPGDNVKALAEAVRENFDHLTKK
                     SVWFFGGDGWAYDIGYGGLDHVLASGADVNVMVFDTEIYSNTGGQASKATPTGAVAQF
                     AAAGKRRKKKDLGMMAMSYGDVYVAQVAMGANPAQFLKAVTEAEAHKGPSLIIAYAPC
                     ISHGLRCGMSDVQGEIKRAVEVGYWQLYRNNPELIKEGKNPFILDSKEPQGDFREFLR
                     GEVRFSSLERTFPEAADALFERTESEAKARYARYKELAEKN"
     gene            12651..13661
                     /locus_tag="ACZPCL_03580"
     CDS             12651..13661
                     /locus_tag="ACZPCL_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1161602.1"
                     /translation="MATIKDVAKQAGVSVTTVSIIINGKAEERKISAATQERVAEAMR
                     DLGYQPNLSARRLRSQENERPVIAFFWPLDYRVSILASFLNFIQIEIAESGFDCEMMI
                     QTYENDKLEQYGTTFLKNGYSGAIIGACSAKDQQWLEGICPRMPVILINRESEHFSTV
                     CTDNDEVGLMAATKIRQKGYTEAAIFASRHSYFATSQRVQAFISSCERLGIETDEKYI
                     LKGSSTRNGGYEIGKQYAALKEPPKMIFCDSDAIALGALRAFHEEGISVPGDAELLSI
                     GMLDPEAASYYVPSLSVVEMPNKEIGQQVLRLMRKKVLNNDISSEHVKVHAKLMLRES
                     FS"
     gene            14176..15588
                     /locus_tag="ACZPCL_03585"
     CDS             14176..15588
                     /locus_tag="ACZPCL_03585"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HIR1161603.1"
                     /translation="MFWNKKKAPELSAPSGIGAADFAGAGTPLKFNSVDFRDGQQSLF
                     ATRLTTADMIPLLEQMDAVGYDSMEMWGGATFDVAVRFLKDDPWERVRTFKKYVKKTP
                     LKMVLRGQNLVGYKAYPDDVVEAFIAQAAEAGIDVFLTFDALQDLRNCETAFKAIKKA
                     GKKIEGSVQYNVSPFHTTEVFVQNALEQEQMGASLMHVEDMAGLMTPEAAYELITALK
                     KALKIPVHLHCHCTGGMAEMAYWEAIRAGVDGLDVCVSSMSMGPSLPPIESYLAALKG
                     TSRDPKIDLGQFADINKKFAELRRKYADFGTKLVGVDVGCLQHQIPGGMLSNLESQLT
                     AMKLYDRLPEVLEEATRVRADMGYPPLATPSSQMCGAQATTNVLTGNRYAMVSRELKD
                     YCRGLYGRPPGPIDPTLLEKALGDEKPAFIRPGDQLAPGMDTARAEVGSLARTEEDIV
                     TYALFPGYAPDFLKAKYSLQ"
     gene            15692..16090
                     /locus_tag="ACZPCL_03590"
     CDS             15692..16090
                     /locus_tag="ACZPCL_03590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014664.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR1161604.1"
                     /translation="MKQTDEAKLERSREILEALKEARGGKLLDSHRVMGNDPNLINMF
                     YQQYVNCNKKDISIPKKYRELIVMAVGIATSTETTMKVHGKLALENGATIDEIFEVIR
                     ILFFTCGVTKLLPALETLGDLFEAIDLEEK"
     gene            16239..17591
                     /locus_tag="ACZPCL_03595"
     CDS             16239..17591
                     /locus_tag="ACZPCL_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1161605.1"
                     /translation="MKTDSAQIQYDKMTKTPVNKLVLQLGLPTTVSMLVTSVYNMADT
                     FFVSQLGTSVSGATGVVFALMAIINAFGFMFGHGAGSNISRKLGAHDVESARKFASTS
                     FFLSLLCGALICILGFLFHAPFMRLLGSTDSILPHAIEYSTWILIAAPAMASSCVMNN
                     ILRYEGKAFFAMLGLASGGILNIFGDMFLIFVLKLGVRGAGISTAISQYISMAILISP
                     FLRGKVQSRLNIRYVTRQFSDVSNILATGFPSLMRQGLNSVSVMVLNRFAGPYGDAAI
                     AAMSIVTRIINFLFCVALGIGQGFQPVSAFNYGAGKYKRVRNAFWFSVKSSFVVMSIS
                     AFIGITCSAPIVRMFRDDPAVLAIGIPALRIQCIALFFMPLSLYGNMLFQSIGKNGRA
                     TFLSMIRSGLIFIPVLCIMNTILGLTGIQISQTISDIISASITLPMVVKFFKTMPKDK
                     "
     gene            complement(17856..19352)
                     /locus_tag="ACZPCL_03600"
     CDS             complement(17856..19352)
                     /locus_tag="ACZPCL_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014073.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR1161606.1"
                     /translation="MLEMLSIVDLRFLLMVLLGSVAGLFVGAIPGLSVSMATALLVSI
                     TYTWDHSDALAMIMGVYVVGVFSGAVSAILINIPGAPSSVVTTLDGYPMSKKGQSYKA
                     LFTATIYSFVGSVFGFIALGLLAEPVAAIAIKFTKMDYFLLALFGLVTVGSVSTKNYA
                     KGLVSVMLGLIISMVGLDPLMGTKRLTLGIQNLAGGISTVPALVGFFGFAEVLSVIYS
                     LDQEQNVLAMEKTKVSLKEVLKHWKLSIYTSTIGMLVGALPGAGGPVASFIAYNEAKR
                     IVKEPEVPFGEGAVEGIMASESSNNACIGGALIPMLTLAVPGDAVTAIILSVFYVHGL
                     QPGPMFIRKNPEAFQAILIAGIIACFALLVLGLLVAPRISKVITIPKNVMIPVVTSLC
                     VIGSFACNNRLFDVALMFLFGVIGFLMRRFDYPVAPLILAMVLGQMMDSNFRQAVSLA
                     SSADHPILFTFGHWQTVLLLLCIAWVLLGNIPWTKPYVEKVRGVFRRK"
     gene            complement(19372..19893)
                     /locus_tag="ACZPCL_03605"
     CDS             complement(19372..19893)
                     /locus_tag="ACZPCL_03605"
                     /inference="COORDINATES: protein motif:HMM:NF018979.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR1161607.1"
                     /translation="MKNKVSSLIAEGILFCGSGILLLSYSLISYAKAFNKSWSQSPYL
                     FPALVGAALIGLSIWIMGQGVLAMKRAGNGITLKEKTDSKGKMARVLGVLVLCGLYYL
                     VLGAVSIPRVTIGILSFSITISIFEVATVIFLIAMMWFMKVRKVAVLVLVPIGTSLFL
                     SIAFRTCLHVMLP"
     gene            complement(19961..>20653)
                     /locus_tag="ACZPCL_03610"
     CDS             complement(19961..>20653)
                     /locus_tag="ACZPCL_03610"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR1161608.1"
                     /translation="YSDFTVISPLVGDPKVLVVGKDSKYNTLEELLDDIKANPGKITM
                     SHSGPGGSGHNQGLVMKELGYDVAMTSFDSGNDALLGVIGGQVDFTNPNISTLGGYIE
                     SGDVKPLAVFSDKRMEAYPDIPAFTESVPEAEKYLNIPYTLLSFVVNNDTPQDVVDVL
                     KAASQKVFADPEWTDFVKQKAADPVYEEYTDDASIQKFYDNWKSVICWLQYDNGVAEK
                     SPEDFGIARVAE"
BASE COUNT         5606 a         5261 c         4083 g         5703 t
ORIGIN      
        1 ctttatgagg cttaccattt tcatctacag ctacaaaggt aaagaatgct tctgctgtct
       61 ggacctgttc ttcttccggg tcctgatcca tcataactgt gatcttgatc ttcatggaag
      121 tgtttcctac atggacaact tctgcattga tatcaataaa gcttccctga ggcataggac
      181 gcataaaacg aatgctttca atccctactg tcataacttc cgaatgtgca aagcgctttg
      241 ctgcaattcc ggatacttcg tccatccagt caagcagtct tcctccataa agatttcctg
      301 catcattggt tgaagccggc attacgatcc gtgacattct tgtaactggt aattccatca
      361 tattttctcc tttattatga aatatacttc tttaggaaac tgcctcgatc tcatctgcga
      421 tccgttttcc catctctttt gttcctacca ggatctttcc tttatgccag atatctgcag
      481 ttgcaatatt ggccgctaaa acctggcgga tggcttcttc tatggctccg gcctctgcct
      541 ctaagccaaa ggaaaggcgc agcatctggg ctgcagccgc aatcatgcca atgggattga
      601 ccatatcttt tccgatcaca gactcatctg gataatgaag ctggttcggt gtgtaaatcc
      661 cctgctctgt attacttaat tccgcagagg gataaagata gggcgttccg gtcagttctg
      721 ttccttcatc agatattata tcaccaaaca gattggaggt aacaaaaacg tcaaaccgac
      781 ctggatttcg gatcagttcc atggctgcat tatcaatata taaatgttcc agttccacat
      841 ctggaaaatc ctcggaaacc tgctgtacag tctgacgcca gagacgggaa ctgccaagaa
      901 catttgactt gtccagattg gtaactttgt tctttcggct ttttgccagt ttaaaggcag
      961 tataggcagt cttccttaca attgtttcat tataatactc atattcataa gcttcccgac
     1021 cgccgtcccc atttccctgg actttggcag agcagaaaac tccccctgca atatcacgga
     1081 caaaagcaac atccattccc tgctccagac gttctgcttt taagggagaa aaacatttta
     1141 aagaaggata catacgcaca ggacggatat tggtacttac ctgcagtacc tttctcagta
     1201 tcataagagc gccttccgga cgcttatcca acggccagtc ctggtatttt ttcagacctg
     1261 aatttccaaa taagacagcc ggaacctggg tacagatttt taaactttcc tgtggaagag
     1321 gttcttttcc tgcttcaatg gcctgagagc aggctaaaac aggatacagc tttaaatgat
     1381 ggccgaaccg gcggcagact gcctgaagca cccgtactgc ctcttccatc atctccggtc
     1441 ctacaccatc tccctgtatt actgctgctt ccagtttcat attgcatcac accttctctc
     1501 ttcattcttt tccatatcaa cgacttcctt ttatcccaca gatcttctct actgccggtt
     1561 ttaacacctt ctgcatcggc ttcatcagct ttcctaccag caatgcgcat atcacggttc
     1621 cttcccggat gccatccagt tttccaagaa atacaaaaga aaggacaata ccgatcacaa
     1681 cggtcagaca atccatgatg accttaatat catgaaaagg cttgtccggg aagaatacac
     1741 ggtttactgc atgggtcatt ccttccatag gcatatttac cagctgcaca tccatataca
     1801 ggcatacgcc tattgctaca aaaacgatac tgatcgccag cataaccagc tgccccggat
     1861 aggtgggaat ggcaaaatct cccaccactt tctttgcaac atctacaaaa cggccaaaaa
     1921 tcgtggaaaa tatcagctgc gtcaaatcta tgggacggaa tttttttctt aacaataata
     1981 tctgcaccag aatataaaaa gcaaaaatgc cgatcacgca gtttccaaga tccagtcctg
     2041 taatgcggct gatcacatag ggaagggagt ttaccggtga aactcccaga gaagaattaa
     2101 cagaaaatgc cactccaaag gccatgaata aaagtccggt cacatagatc agggcctttt
     2161 ttaccatcag catgtaaatc cactccatac tggaatacct gtataagtct tcgcctcttc
     2221 ttccccgctt aatacacgga tcgcacctgc tgccagtgct tccatttcaa actcgcctgc
     2281 cataacagtg atcggcgcaa taaaggaaag catttctgtc agtttttcta ctacatactt
     2341 gtcatgggag atgccgcctg ttaatacgat cccgtccacc tttccatgaa ggactgctgc
     2401 catagaacct gctgtctttc cgatctcata gatcatggaa tcgtaaatta atttcgcata
     2461 cttattccca ttttcgatca tggcagttac ttctcttgca tcagaagttt ccagatgatc
     2521 taccaggcca ccggtctttg tgatcttctc acgcatctct ttttctgtat atttgccgga
     2581 atagcacatg gtcacaacat cctttacagg aagctggccg cagcgggtgg gggccattgg
     2641 gccgtcacca tttgcaatat cattggaatc aaccattttt cccattctgt gggcagttac
     2701 agaaataccg ccgccaatat gacagatgat caggttcata tcttcatatt tctttccctg
     2761 ctttgctgca aaacggatac cgatctcctt ctggtttaat gcatggatac ggctctctct
     2821 gtatacaccc tttaagccgg ttacacgggc tacatcctgg aactcatcta catctggtgg
     2881 atttactaca aaagcagctc ccccgaattc tttagaaaat gcatctgcca gctgtgcgcc
     2941 taaggttgct ggatgcttta ctgtatagca ggttcttgca tgttcaagaa gcttgtcacc
     3001 aatagcgaaa acgccgcctt ctacatttac caggccgccg cctcttgcgg aaaatgcgtc
     3061 tacgccttct aaggtttgtc cttcttcttt caggacgtct aatatcattt cctttctata
     3121 tggcagctgg tcgctgatct ccttaaattc ttttaatttt tctgcatcat ggcttatatt
     3181 tctggaaaaa atttcctttt ctccctcaaa catagcaatc tttgtggaag tagagccggg
     3241 gttgatagca aatactttat agtttttcat aatatatttc tcctgttatc taatatcttt
     3301 aaaggggttt tctaaaaaag cacgctgaca tagcgccaac gtgccttgtt ttttgttgca
     3361 atgtgcctgc cttaatggaa cagtggatat aacagtccga tccagataac catcagtgcg
     3421 ccgccgatac gggtagaaat agaagcgaat gccagcaggt tcatacggtt tgctgcagat
     3481 aaaactgcca ggtcaccgga accaccgatg ttgcagcagc aaagacctgc tgtaacgcct
     3541 gcctcgtatg cccacagacc ggagagcttt ccgaagatca ttgcgcctaa cattgcacca
     3601 aatactgcta agaagataac aacgaatgcg cctgggctga agtattctgc cagagtggag
     3661 aacttcaggg agttgatacc gatacctgcg atcagcattg gaagcagtgc ttttaatgca
     3721 aagttcatgg agtatacaca attgtcagca aactggtctg gaagaatacc ggtaaatttg
     3781 atgataatgc caaggataat ggtccatgcc agtccaggga tcacgctgaa gcctggaatg
     3841 tggccaagga tattacccag taagtacaga cagaagctca taaaaatacc agaacctaat
     3901 gctacataat cagcagaagt accaggtctc ttctgttcct gtgcttcctc tttctgtacc
     3961 tgggcactct ttgcagcaag gatctcgccg ttaccattta agcccttcat ttttgcagtt
     4021 acagaacctg cgattgctgc taatacaaca gccagtacgt ttgcgataga tgcgtagcaa
     4081 aggaattttc ctgccattgg agtcatatct gtaccggaaa gactggaata cagagctggc
     4141 agagtggtca tagcaccgcc ggaaccaccg gacatacaag gagcaccgat atcaaacagt
     4201 gcctccggaa taccatagcc tgtaaccaga cctgctaaca tacagaagcc taatgcaaat
     4261 acctggcttc caaggatagt cggcatgtat cttgcagttg cgccaagtaa aaccttacgg
     4321 tccataacaa ggatggagcc aactaacagc atggcaatat atgcatcctg gaaaccgccg
     4381 cccataagga ccttaactcc gtttaccagt tcctgaggaa ccagaccaac gtaatttaag
     4441 aaagaagcac cgatcagagg cagaagacat gcaccgccta agtagttatt tacgatcggg
     4501 atggtatttc caagccagaa gaacagaccg ccaactgcca tcagataagc gatggtcatg
     4561 ataaagctgg ttgccatgta cttgcctgca tcattggaat agatcttaac aaccggcata
     4621 aagccaaggt aaacagttgc cattacaatg ataaagaatg gaatgaaata ctttgcagga
     4681 agcccacaca ccttaaaatt taattttttc tccattgttt ttccccctat tatctgctgc
     4741 ccactaacgc cgcaatgacg atggacatat atttatcatc tgcatttgct gcacgggata
     4801 ccaacaggac cggaaccttt gctcccagta ccacgccgcc ggttcttccg ccaccaatta
     4861 cagtgatggt ctttgctgca atatttccgc ttacaatatc cggaacaact aaaagatctg
     4921 catctcctgc aacaggactt acatagcctt taattggtgc ggaagcagga tccatagcca
     4981 ggtcatagct gatcgggcct tcgatgatgc agccttctac gccttcttcc ttgatctcag
     5041 cagcttctac ggtttccttc atcttaggat ttactttttc tactgctgca aggatagcta
     5101 cctttggctg ttctacgcct aatgcatgga atgcttctac tgcattctgg atagctgctt
     5161 tcttctgatc tttggaagga taagtaagaa ggcctacatc agtaatagcg aataccttgt
     5221 ggtaattcgg gctttccatg aaagcaagta aactcatggt atcgttcttt ctgatccctc
     5281 tttcacggtt taccagtacc ttcatcaggg ctcctgtctc tgtttttccc ttcataatgc
     5341 agtctacttc accgtctctt gccatatcag cagccttctg gatggcttcc gtaggatctt
     5401 ccacatggat aatagtcatc ttatctgcat ctgtgccgat ctgagccagg atctcacgga
     5461 tctttggctc atcaccgatc agaaccggct caaccatgcc gtccctataa gcatgactga
     5521 tcgcttccag tgtatgttcg tcctgagccg gtacaactgc aactttgcgc tttaccggca
     5581 tagcttttaa catttctttc agctgttcaa aattctttat cattgatttt cctcctgttt
     5641 atagatgttc tcccaggatc agatacagaa agatcctaag agaacatctg accgcttatc
     5701 tttaagccag tgctggcaga tcagccggat ctgttacaac acccataact gcggttgctg
     5761 ctgctaccgt aggacttgac agataaccta aggttttctt ggttccctga cgtccgatga
     5821 agttacggtt tgcagtcagc agaatccttt catcatctgt catcagacca tatggcatac
     5881 cacagcaaag tccgcagcat ggatgacaga tcactgcacc tgcctcaata aaagtcttaa
     5941 tatatcctct ttcaatggcc tgtttgaaaa tggtattagt tgcaggaaca acgataaaac
     6001 gcagtccttt tgccacttta cggcctttta agatctttgc agctgcttcc atatcttcaa
     6061 tacttccgtt ggtgcagctt cccagatata cctcatccat tttggtgcct tttacttcac
     6121 tgatggggtg aacattgtca acgccctgtg gacaggaaag ctgaggttcc agttcgctta
     6181 catcataggt aagtaccttt tcatactttg cttcgtcatc tacgcatacc cagtcgatat
     6241 cttctaatgg cattccgtag tactctgctg tcttttcgtc agcctcaaac agaccgcact
     6301 ttgcgcctgc ttccagagcc atgtttgcaa ctgtaaaacg ctgttccatg ctcatttcat
     6361 gggctgcagg accggtaaat tccagagatt tgtactgtcc gccgtcggcc ttgatatcac
     6421 ctaagatacg aaggataacg tccttactca taacaccgtc tcttaaatgg ccgtttaata
     6481 cgatcttgat agcctctgga accttgaacc aaagttctcc ggtaataaga gctgctgcca
     6541 tttctgctgt accaatacca gagcagaagt ttccggcacc gccataagta gttgtatggc
     6601 tgtcggttgc agttgcgatt tcgcctggct ttgcatactt tgcttcatgc ataatggtgt
     6661 ggcagatgcc ttctccaatg tgaagcttct taattccata ggtttttgcc agttccagac
     6721 cagcttcata atggtcatag tcatttttag caacggcagt tggcatacag tgatcccaga
     6781 cgatggttac cttatctgga tcgtcgatct tgtctgcacc aatgtctttc atggtttcta
     6841 acagataagt agtataaata tcatggatca tgaacatatc tggctttgtg atcacgatat
     6901 ctccaggagc cacatgttct tttcctgcat ttttctttaa tatcttttct accatggtgt
     6961 agcctttttt acggcactgt tccatggaaa gaggcttgat atccattttc acatttccag
     7021 ctgcaagacg cttctttgta tcttcgatca gtccgccgtt tgctacaatg ttatacagat
     7081 tttcaggaac tgcaccaacc ttgaaggtct ttccatttac actgaccgtc tgtgcgtcta
     7141 catctaccgt aacaatatct ccgccttcac acacgtcctg gatctgatca caggtaagaa
     7201 gaaggatgcc gctgttaaat gcatttctga agaagattct tgcaaaactc tttgcgatga
     7261 tgcaacggac accattgtaa gaaagaacgg tagctgcctg ctcacggctg gaaccacagc
     7321 caaagttggt tcctgctacc aggatgtctc ctggctgaac ggttgaacca aattccggct
     7381 ttaacagctc aaatgcatat tttttcatct cgttacgatc cgggatagta cctctttttc
     7441 cagggataat ggtatctgta tcaatatcat ttccgaattt ccagattttt ccactgaact
     7501 tcttttccat agctgtctcc ttacatctgc ttcgcaaagg aagcgttcat tactgtttca
     7561 acagagcctg tataaacttc tgcgtcatcg cagcccatag aacctgtaaa ggtgtacagt
     7621 ccggtggtta ccagacgttc cttatgtccc atagctccag gtccctggat cacaacgctg
     7681 tggtcccctg ctgcgttgat ctgtgctccg aaatcaatga actttgtaag aatgccttcc
     7741 tctgcagcct gaagataatc acggctggtt gccgggcaga tgcttaaacg gaagcctaat
     7801 gccagcttgt gtccatctaa catagaagca gcttttcgta actcctcaat agttccgcct
     7861 gtatagccgc cgatctggcc tgcatttaat tccatgccag ctttagaagc tctagaagca
     7921 attccagcct tcttctggtc atttctatca ccacatggaa gcatgaccat tggctctaca
     7981 gttgccagat ttaaggttaa accagcagac tgtttttctt ctgtaatgga agcagtaaat
     8041 gcccctgtaa tgcatgccat gctgcagaat actgctttct catgtgcatc aaaggatggt
     8101 gcataaactt ccactgcctt gcggtttgac tcatgatctt ctgccaggaa tgcaagtgct
     8161 gcgtccataa cagttacgcc ttcttttaag ctgccttcca ggtttacata aactgtttcc
     8221 ggaacaatga tgctgtaacg gtctgtttca gctgctcttg ccagctcagg aatgcttacg
     8281 ttgatgccca gagcaccgat gcttccgaaa atacttccgt gggaaccgcc gcctacaatg
     8341 atctggcccg gttttaccac ttcatttaac atatactgat atccaacacc ttctgcctgg
     8401 atataaggac atccgtactt ttcagcaaat gccaggttct ttcttaaaat agctgctgct
     8461 tccgggcgtc cggttggtac atcatggtca tagatgacca ttaccttttc aggttttgct
     8521 acggcagaga tttcctctac cgcagcatga gatacaccat cattgataac gatccagtca
     8581 ggttctactg ttacaactgt accggctttt cccccaagta accgttctac aaatgtactc
     8641 ataatcttct tctcctgttt tagttctttt cagcaagttc tttgtatctt gcgtaacgtg
     8701 cctttgcttc gctttctgta cgctcaaaca gtgcatcggc tgcctctggg aaggtacgct
     8761 ccagagatga gaaacgaacc tcgcctctta agaactctct aaaatcgccc tgaggttcct
     8821 tggaatccag gataaatgga ttcttgcctt ccttgatcag ttctgggttg tttctgtata
     8881 actgccagta accaacttca actgcacgct tgatctcgcc ctgaacatca ctcattccgc
     8941 agcgcagtcc gtggctgata catggagcat aggcaatgat cagagatggt cccttgtgag
     9001 cttctgcttc tgttactgcc tttaagaact gtgcaggatt tgcacccata gctacctgtg
     9061 ctacatatac atcgccgtag ctcattgcca tcatgcctaa gtctttcttc tttctgcgct
     9121 taccagctgc cgcaaactgc gcaactgcac cggtaggagt tgccttagat gcctgaccgc
     9181 ctgtattgga gtagatctcg gtatcgaata ccattacatt tacgtctgcg ccggaagcta
     9241 atacatggtc taagccgccg taaccgatat cgtatgccca gccgtcaccg ccgaagaacc
     9301 atacggattt cttggtcaga tggtcaaagt tttcacgaac tgcctcagcc agtgccttta
     9361 cattatctcc aggaacattt gctgcaacca gatctgcatt ttcacttaat aaaccgcttt
     9421 ccaggatggc attctttaat gcttcgcctg tttcacggga gctcttgtca ttgtaagatg
     9481 ccagccaagc tgctgctgtc tctttgatct tggcatcatc tgtcttttca gcaagttcct
     9541 ctacatgctt agccagcttt tctctctgct gttcaacaga aagccagata cccataccat
     9601 actcagcgtt gttctcaaat aaggagccgc ctactgcagg tccctgtccc ttgtggttgg
     9661 tggtatatgg gaaggaaggg aagctggtgg accatacctg ggagcagcct gttgcagttg
     9721 ctacatacat acggtcacca aacagctgtg tgatcagctt tgcataagga gtttcgccgc
     9781 atcctgggca ggagcctggg aactctaaca gtggctgctc aaactggctg cccttaacag
     9841 ttgttgttcc aaacggattt gcctttggag ctaacttcat tgcatattcc cagttatctg
     9901 cttcatttaa gttgttgtct aatggcttca tggtaatagc cttttcctta gacgggcata
     9961 catttgcaca gcttccgcag cctgtacagt caagagcgga tacctgtaaa cggaaacgta
    10021 agccttctgc gcctttgccc ttaacagcag ttgttgtaaa ggtttcagga gcctttgctg
    10081 cctcatcctc atttacaagg aatggacgga tacaggaatg agggcagacg taggaacaca
    10141 tattacactg gatacatttc tcgctgtccc aaacaggaat attaactgca atacctctct
    10201 tttcgtactt ggaagtaccc tgaggcatgg ttccgtctgg cagatcggtg aaatcgctga
    10261 caggaatatt atcgcccttc atagcattta caggcatctg gatcttgcgg atccactttg
    10321 gcaggttctc atcgatcttc tcttctggaa tttcgatatc agcccatctt gcaggtactt
    10381 ctgcctcttc taccatttct acgcccttat caacagctgc gtagttcatg ttgacgatct
    10441 tttcgccctt tcttccgtag gactttaaga tagctttctt catatatcca actgcttctt
    10501 cgatcgggat aatacctgtg atcttgaaga atgcagcctg taaaatggta ttggtcctgc
    10561 ttcccagtcc cagttctgca gcaattgcag tggaatcaat ggtatacaga tggatcttct
    10621 ttaatgctgc tgctttctta aatgcaggag ttaaatgctt ttctaactct tcgcccttcc
    10681 agtcgcaggt gatcagcaca tttgcgcctt cctttgcatc tgccaggata tcatagcggt
    10741 ccagatagga cttattgtgg caggcaatga aatctgcgtg gtttacaagg taggtagagt
    10801 taatcggctt cttgccaaaa cgtaagtggg aacgggtaac accaccggac ttcttgccgt
    10861 catactcaaa gtaagcctga acatttaagt ctgtgtggtc accgatgatc ttaatggtgt
    10921 tcttatttgc accaacggta ccgtcagaac caaggcccca gaacttgcag ctgatggttc
    10981 cttccggaac agtatttact tctggtccta caggcagtga cagatgggtc acatcgtcat
    11041 taatacctac ggtataatgg ttctttggct ctgctgcctt catattgtca aactgtgcga
    11101 taacctggtc tggagtataa tccttgcttg ccagaccata acgacatgct aaaaccttga
    11161 tatttctgcc tgactccagg atcacagaac atacatcctc gtacagcggc tcgcctgctg
    11221 cacccggctc cttggtacgg tcgccaacag tgatcatctt tacagtctca ggaagctggc
    11281 ttaagaaata ctttgctgag aatgggcggt acagatgagc atcaatgaaa ccaacctttt
    11341 cgccctttgc atttaagtaa tctacagttt cgcaaagaac gccgcttgca gatcccatta
    11401 ctacgatcac acgctctgca tccggagcac catagtagtt aaatggctta taatctcttc
    11461 ctgtgatctt gctgatctcg ttcatgtaat ctgcaacgat atccggcagt gcatcataat
    11521 aggtattgca tgcttcacgg ctctggaaat atgtatcaga atactgacct gtggttctta
    11581 aaacaggatg ctctggattt aatgcattct cacggaattt ttccagttcc ttccagtcaa
    11641 ccagtttctt taaatcttcg taatccagac attctacttt ctggatctca tgggaagtac
    11701 ggaaaccatc aaagaaatga aggaaaggta cgtggccttt aatagctgcc agatgagcaa
    11761 ctgcacctaa atccattgct tcctgtggtg aagaggatgc taacattgca tatccggtct
    11821 gacgacaggc cataacatcg gaatgctctg cgttaataga aatcgcatgg gttgccacgt
    11881 ttctggatgc tacatggata acacctggct ttaactgacc tgcaatacgg tacatggtcg
    11941 ggatcattaa cataagtccc tgggatgctg tataggaagt tgccagtaca ccgccgtcta
    12001 atgcaccgtg tactgcggaa acagctccgc attcagactg catctcaacc agctttacct
    12061 gctggccgaa catgttcttc attccatttg ctgaccactt gtcaaccagt tcagccattg
    12121 gagaagatgg tgtgatcgga tagatcgctg ctacttcggt aaaagcgtag gaagcgtaag
    12181 ctgctgcttc gtttccgtcc attgctctgc ttactttact cataatctgt actttccttt
    12241 ctttgtgggg ttgatcgttt ttaattagtt atcatttttc atctctgtca gatctctctt
    12301 ccccagaaat cggacagccg gcgaaatgcc ttgcttttgc ggatatgtac ttgtgcatct
    12361 ttgtatctct tgtattgcat ttatttagta gtaacatgta catatctcat ttagtttaac
    12421 gtgttagtaa aacgtttatc cttgtcacaa ataatagttt atcgtttgat gtttgtcaag
    12481 tgttttgata aaaatacaat attctttgtc agttttatat agtttaacgt attacttatt
    12541 gtgcattttt tatggagggg atatatcctt attctttcct gcttgacata atgtgtgaat
    12601 ctggcttaag attgatttta gtaaacaaaa aaaggtataa aggtgaatat atggcaacta
    12661 taaaagatgt ggcaaaacag gccggtgtat ccgttacaac cgtgtccatt atcataaacg
    12721 gaaaagcaga agaaagaaaa atctccgctg ctacccagga acgggtggcc gaagcaatgc
    12781 gggatttagg ctatcagcca aacttaagcg caagacgtct gcgctcccag gaaaatgaac
    12841 gccctgtgat cgcatttttc tggccattgg actatcgtgt ttccattctg gcttctttct
    12901 taaatttcat tcagatagaa attgccgaat ctggctttga ctgtgaaatg atgatacaga
    12961 cttatgaaaa tgataaactg gaacaatatg gtaccacttt cttaaaaaat ggctacagcg
    13021 gcgctattat cggtgcatgc tctgccaaag accagcagtg gcttgagggc atctgccccc
    13081 gtatgccagt gatactgatc aaccgtgagt ccgaacattt ttccactgta tgcaccgata
    13141 atgacgaagt cggcctgatg gcagccacaa agatccgcca gaaaggttat acagaagcag
    13201 ctatctttgc atccagacac tcctactttg ctaccagtca gcgtgtccag gcatttataa
    13261 gttcctgcga acgtctggga attgaaacag acgaaaaata cattctgaag ggatccagta
    13321 cacgaaacgg cggttatgag atcggcaaac aatacgctgc ccttaaggaa ccaccaaaaa
    13381 tgatcttctg tgactccgat gccattgccc tgggtgcctt aagagctttc catgaagaag
    13441 ggatctctgt tccgggagat gcagaacttc tctccattgg tatgttggat ccggaagctg
    13501 cttcctacta tgtcccatcc cttagtgtag tagaaatgcc aaacaaagag atcggtcagc
    13561 aagttctgcg cctgatgaga aaaaaagttt taaacaatga tatatcatca gagcatgtga
    13621 aagtacatgc gaaactgatg ctgagagaga gtttttcata atccattcgt catctattat
    13681 tttttgcagg agctgtaaat atcagctcct gtctttttat ctcattacta tgttatagtt
    13741 tccacaacac gtaatcttct ttttcacgtt ttttcatttt ccctcttgac aacctcattc
    13801 ttttttagta ttcttgtttt aacgttttag tttaacgttt gacttaagta ttacatatcc
    13861 tacatgattc aatctttgca ggtttctgac atgcatactt ccccttttgc atctgccagt
    13921 acctgccgtt ctctggccag ctttcagtac ctggtccctt ccggattctt tttttgattt
    13981 cctgccggaa cctgccattt tccaggtact gtaaactgac cagagaacga tctccaaaaa
    14041 atcaagaaaa tccaacattc cccgacaggt tttctctttg atccacctgt caacagcaaa
    14101 caactataat aaataatcaa aggagcatgt caccttgcgg tgacatcctt attacgaaat
    14161 gaaaggtgtg gtattttgtt ctggaacaaa aaaaaggctc ctgaactttc tgctccttcc
    14221 ggaatcggag cagcagactt tgcaggcgca ggaactcctc ttaaattcaa cagtgtagat
    14281 ttccgcgacg gacagcagtc tctttttgca acccgtctta ccactgcaga tatgatcccg
    14341 ctcttagagc agatggacgc agttggatat gactccatgg aaatgtgggg cggcgctacc
    14401 ttcgatgtag cagtccgttt cctgaaagac gatccatggg agcgcgtaag aacctttaaa
    14461 aaatacgtaa agaaaacccc attaaaaatg gtactccgcg gccagaacct ggttggctac
    14521 aaagcatatc cagatgacgt agtagaagcc ttcatcgcac aggcagcaga agccggaatc
    14581 gatgtattcc ttaccttcga tgcccttcag gacctgagaa actgcgaaac cgcattcaaa
    14641 gccatcaaaa aagcaggcaa aaagatcgaa ggttccgttc agtataatgt aagcccattc
    14701 catacaacag aagtattcgt ccagaatgcc ttagaacagg aacagatggg tgcatccctg
    14761 atgcacgtag aagatatggc cggacttatg acaccggaag ctgcctacga actgatcacc
    14821 gctttgaaaa aagcccttaa gatcccggta catcttcact gccactgcac aggcggtatg
    14881 gcagaaatgg cttactggga agccatccgc gccggtgtag acggactgga tgtatgtgtt
    14941 tcctctatgt ccatgggacc atcccttccg ccaatcgaaa gctacctggc agctttaaag
    15001 ggaaccagca gagatccgaa gatcgatctt ggacagttcg ctgatatcaa caaaaaattt
    15061 gctgaacttc gccgcaaata tgcagatttc ggcaccaaac tggtaggtgt agacgttggc
    15121 tgtctccagc atcagatccc aggcggtatg ctctccaacc tggaaagcca gcttacagcc
    15181 atgaaactgt atgaccgtct cccagaagta ttagaagaag caacccgtgt ccgtgctgat
    15241 atgggatatc cgcctcttgc caccccatcc agccagatgt gcggtgccca ggcaaccaca
    15301 aacgtattaa ccggcaaccg ctacgcaatg gtcagccgtg aattaaaaga ctactgccgc
    15361 ggtctctacg gacgcccacc gggccctatt gatccaaccc ttcttgaaaa ggcccttgga
    15421 gatgaaaaac cggcattcat ccgtcctggt gaccagttag ctccaggaat ggacacagca
    15481 agagccgaag tcggctcact tgcccgcaca gaagaagaca tagtcaccta tgccctcttc
    15541 ccaggctatg ccccagattt tctgaaagct aaatacagct tgcagtaaat cccattttat
    15601 tttatccact cacgaacacg cactaacgcg ctccactgcc tgctgacgca ggcggtatgt
    15661 ttatcaataa taagtgaaag aaggtattac gttgaaacag acagacgaag ctaaacttga
    15721 gcgttccaga gagatcctgg aagctttaaa agaagcccgc ggaggaaaac ttttagactc
    15781 ccaccgcgtt atgggaaatg acccgaacct gatcaatatg ttctatcagc aatatgtaaa
    15841 ctgcaacaaa aaagacatct ctattcctaa aaagtacaga gaactgatcg tcatggcagt
    15901 cggaattgca acctccacag agaccactat gaaagtccat ggaaaactgg ctctggaaaa
    15961 cggcgctacc attgatgaga tctttgaagt tatccgcatc ctgttcttca cctgcggcgt
    16021 aacaaaactg cttccagcac tggaaaccct cggagatctt ttcgaagcaa tcgacctgga
    16081 agaaaaataa atataaacac cgctatgtaa aaacaaagta aacttttttt aactgaacag
    16141 tataaaaacg cttttcaaaa ggcctttcta tgataaaata aatcacagga gggcctttta
    16201 ttctatttcc cacacttata tataaaggta tacatattat gaaaacagat agtgcgcaga
    16261 tacaatatga taaaatgaca aaaacccccg tcaacaaact ggtactgcag cttggactgc
    16321 caaccacagt gagtatgctt gtgaccagtg tatacaatat ggcagatacc ttctttgtaa
    16381 gccagctcgg aaccagtgtc agcggtgcta caggagtcgt gttcgccctg atggctatta
    16441 tcaatgcctt cggcttcatg ttcggacatg gcgcaggcag caatatcagc cgtaaactgg
    16501 gagcgcatga cgtagaaagc gcccgcaaat tcgcatccac cagcttcttc ttatcattat
    16561 tatgtggcgc cctcatttgc atccttggct ttctcttcca cgctcccttc atgcgtctcc
    16621 ttggaagcac tgactccatc cttcctcacg cgatcgaata tagcacctgg atccttattg
    16681 cagcaccagc catggcatcc agctgcgtta tgaataacat tctccgctac gaaggaaaag
    16741 ccttctttgc catgctaggt cttgcctccg gcggtatttt aaacatcttc ggagacatgt
    16801 tcctgatctt cgtcctaaaa ctgggagtcc gtggcgctgg tatctccacc gctatttccc
    16861 agtacatcag catggccatc ctcatcagcc cattccttcg cggaaaagtc caaagccgcc
    16921 taaacatccg ctacgtcacc cgacagttca gcgatgtgtc caacatcctg gcaaccggct
    16981 ttccaagcct gatgcgccag ggacttaaca gcgtttccgt tatggtatta aaccgctttg
    17041 caggccctta cggtgatgcc gctatcgcag ccatgagcat tgtcaccagg atcataaact
    17101 tcctcttctg tgtagcactg ggcataggac aaggcttcca gccagtcagc gccttcaact
    17161 acggcgcagg aaaatacaaa cgtgtccgta atgctttctg gttcagcgta aaaagcagct
    17221 tcgtagtaat gagcatttcc gcctttatcg gaataacctg ctctgccccc atcgtgcgca
    17281 tgttccgtga tgacccggca gtccttgcca tcggtattcc cgccctgcgt atccagtgca
    17341 tcgccctctt cttcatgcca ctttccctct atggaaacat gctcttccag agcattggaa
    17401 agaatggccg tgctaccttc ctgtccatga tcagaagcgg actcattttc attcccgtcc
    17461 tctgtatcat gaacactatc cttggactca ccggaattca gatatcccag accatctctg
    17521 atatcatatc cgcttctatc acacttccga tggtcgtaaa attctttaaa accatgccta
    17581 aagacaaata aaatttccat caaaaaacct ctcggactgc gtccgagagg ttggcaacac
    17641 actagctgaa aagaggatga ggtacatgag aagcatccat ttcagaaaaa agacctgttt
    17701 tgtgagtata gcgtcaggtc tgtgatacac tgtctgaaca tgcgcagcat gtgagtttgt
    17761 atcacagacc tgacaaaaag cgacggagca aaacaggtct tttttcgccg ggatgcgctt
    17821 atgtacctca tcctcttttc tttcgaatat gtgccctatt tcctacgaaa cacccctcta
    17881 accttctcca cataaggctt cgtccaagga atatttccca aaagcaccca ggcaatacaa
    17941 agcagcagca acaccgtctg ccaatgccca aacgtaaaca aaatcggatg atctgcagaa
    18001 gaagccaatg ataccgcctg tctaaagtta gaatccatca tctgtcccaa aaccatagcc
    18061 aggatcagcg gtgcaaccgg ataatcaaac cttctcatca aaaatccgat cactccaaac
    18121 aagaacatca aagccacatc aaacaaccgg ttattacatg caaaagatcc gatcacacaa
    18181 agactggtca caaccgggat catcacattc ttcggtatcg tgatcacctt actgatcctc
    18241 ggcgctacca acaatccaag taccaacagc gcgaagcagg caataatccc cgcgatcaaa
    18301 atagcctgaa acgcctccgg atttttcctg ataaacatag gtcccggctg cagtccatga
    18361 acatagaata cagacaaaat gatcgcagta accgcatcac caggcacagc cagcgtcagc
    18421 atcggaataa gcgcaccacc aatacatgca ttattagaag actcactggc cataatacct
    18481 tccacagcac cttcaccaaa tggcacctcc ggttccttca caatacgctt cgcctcatta
    18541 taagcaataa acgaagccac cggaccaccg gcaccaggaa gcgcaccaac cagcatacca
    18601 atcgtcgaag tataaataga cagtttccaa tgtttaagca cttccttcaa actaaccttc
    18661 gtcttctcca tagccagaac attctgttcc tgatcaagac tataaataac actcaacacc
    18721 tcagcaaatc caaaaaagcc aaccagcgcc ggtaccgtac tgataccacc tgctaaattc
    18781 tggatcccca aagtcagtct cttcgtaccc attaacggat caagtcccac catgctgatg
    18841 atcagtccca gcatgacgct gaccaatccc tttgcataat tcttcgtgct gacagatcca
    18901 actgtcacca gtccaaacaa agccaacagg aaataatcca ttttcgtaaa cttaatagca
    18961 atcgccgcaa ccggctcagc caacagtccc aaagcaataa atccaaaaac actacccacg
    19021 aaagaataga tcgtagccgt aaaaagcgcc ttataagact gtcccttctt agacatagga
    19081 taaccatcca aagtagtaac aactgaagac ggcgcaccag gaatattgat caaaatagca
    19141 gaaacagcac ctgagaatac acccaccaca taaacgccca tgatcatagc caaagcgtca
    19201 gaatgatccc aggtataagt aatagaaaca agcagcgctg tagccatgga tacagaaagt
    19261 cccggtatag cacctacaaa aagacctgcc acagatccca aaaggaccat aagaagaaag
    19321 cgcagatcca cgatcgataa catttccaac atctgttttt cctccttaaa cttatggcag
    19381 cattacatgt aaacaagtcc taaatgcaat actcaaaaac aatgatgtcc caataggaac
    19441 aagtacaagc acagccactt ttctcacctt cataaaccac atcatagcga tcagaaaaat
    19501 aaccgtagca acctcaaaaa tagagatcgt aatactaaaa ctcaagattc cgatcgtaac
    19561 ccgcggaatt gaaaccgctc ccagaaccag ataataaagt ccacaaagca ccagcactcc
    19621 caaaactctt gccatctttc ccttactatc tgtcttttct ttcaaagtaa ttccattccc
    19681 ggctctcttc atagccagaa ctccctgtcc catgatccag atagaaagcc cgatcagtgc
    19741 tgcaccaacc aatgccggaa aaagatacgg agactggctc caagatttat taaacgcctt
    19801 tgcataggat ataagagaat acgagagcag caaaatgccg ctcccgcaaa ataagatacc
    19861 ctctgcaatc agtgaagaaa ctttgttttt cattgatcaa tcctcaatta actctcaaca
    19921 tttccagatt acagttccac catatgctga actgtaacta tcactctgca actcttgcaa
    19981 taccaaaatc ctcagggctc ttctcagcta cgccgttgtc atactgtaac cagcagataa
    20041 cagacttcca gttatcatag aacttctgga tagaagcatc atctgtatac tcttcataaa
    20101 caggatcagc tgctttctgc ttaacaaaat cagtccactc aggatcagca aataccttct
    20161 gggatgctgc ctttaataca tcaaccacat cctgtggagt atcattatta acaacaaagc
    20221 ttaacagagt ataaggaata tttaaatact tctcagcttc cggtacagac tcagtaaatg
    20281 caggaatgtc aggataagct tccattctct tatcagagaa aacagctaaa ggcttaacat
    20341 cgccactttc aatatagccg cctaaagtag agatattagg gttagtaaaa tcaacctgtc
    20401 cgccaattac gcctaacaga gcatcattac cggagtcaaa actggtcata gccacatcat
    20461 aaccaagctc cttcataaca agtccctggt tgtgtccgct tccgccagga ccagagtgag
    20521 acatagtgat ctttccagga tttgccttaa tatcatccag taactcttcc agagtgttgt
    20581 acttggagtc tttaccaaca accagtacct taggatctcc tactaatgga gaaataacag
    20641 taaaatcaga ata
//
LOCUS       DBMDUA010000026        20566 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1604_length_20566_cov_2.633814, whole genome shotgun sequence.
ACCESSION   DBMDUA010000026 DBMDUA010000000
VERSION     DBMDUA010000026.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 20566)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20566)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20566
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1604_length_20566_cov_2.633814"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="ACZPCL_03615"
     CDS             <1..604
                     /locus_tag="ACZPCL_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR1161609.1"
                     /translation="LYLLEQKMPIDLLILPAGGKGGEIYGQLEQLQTEAGGKTYYMHQ
                     GDKIKAGEMEFTCIFEKETEEERNAHSLVLCSHYKDLHILFTGDMGISEETELLDLAE
                     ENGSIQQEHLEHVQILKTAHHGSKGSSSPAFLEAMPLKAAFISYGKDNSYGHPSGQVT
                     EEMKKQNISLYETGGKGAWTLESKARNVIIKRTVKSRGEN"
     gene            700..1308
                     /locus_tag="ACZPCL_03620"
     CDS             700..1308
                     /locus_tag="ACZPCL_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR1161610.1"
                     /translation="MSEKNENVKIQVASVEDAEELLAIYAPYVEKTAITFEYEVPSVE
                     EFKGRIAHVLQRFPYLKAVEDGKILGYAYVSPFKERAAYGWSVETSIYVDENVKHKGV
                     GRKLHEVLEAVLKEQGILNMEACIGYPEVEDEYLDKNSVNFHAHMGYRMIGEFKKCGY
                     KFDRWYNMVWMEYIIGEHQAHMTAPESFPQIRDRLKEKYGLE"
     gene            1480..2970
                     /locus_tag="ACZPCL_03625"
     CDS             1480..2970
                     /locus_tag="ACZPCL_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1161611.1"
                     /translation="MPVFDFNDEPKEENKAPECTYTVIRSNETTASPEHPILVLDNSN
                     RKWEHHSWGVFTNPIRKTAFEFQVEGGGTVSADILKVDARFVSLLKWLGESHINVRLS
                     GSNEADGYAVYKIREIAFGGGTKLSAEDGFLQYMIERLQASSAPAEEIREEDADEDGD
                     DMKLTSLQSITDFMTCAGRTLPDNIRLWARRNLAVANSHEVSPEERRHAQRALSIMMN
                     IQWKSNYFEAIDPDEARRILDEELYGMERVKQRIIETIIQINRTHTLPAYGILLIGPA
                     GTGKSQIAYAVARILKLPWTTLDMSSINDPEQLTGSSRVYANAKPGIIMEAFSLSGQS
                     NLVFIINELDKAASGKGNGNPADVLLTLLDNLGFTDNYMECMVPTAGVYPIATANDRS
                     LISAPLMSRFAVIDIPDYTEDEKKIIFTKFALPKILKRMSMKEDECVILPEALDEVVR
                     HYSDTTGIRDLEQAAEHIAANALYQIEVNHVDQVVFDAKMVRELLG"
     gene            2983..3741
                     /locus_tag="ACZPCL_03630"
     CDS             2983..3741
                     /locus_tag="ACZPCL_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004462115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161612.1"
                     /translation="MKHVKDKMIPAVLILVLLVIWEAAVDLLHIPLYVLPSPLEVVKA
                     LFTEGTELFSHGLTTVGEGLLGILIASALSLVLGISMDWFPAVRKGLYPILVVTQTVP
                     MIVMAPILIIYMGFGMAPKILTVVLMCFFPVAVSFADGLQRVDEEYVHLVRSYGADRW
                     KAYCLVKIPAALPELFSGLKVAATYSISGAVVGEWLGSQKGLGYYLLRVKNSYMLDKV
                     FACVVVIILLSLAMNGLIRVIAKVSMPYERNKRS"
     gene            3822..4892
                     /locus_tag="ACZPCL_03635"
     CDS             3822..4892
                     /locus_tag="ACZPCL_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1161613.1"
                     /translation="MKKSWKKASAIFMAAALAAGLAGCGASNNNNTATTAAGKSAAES
                     KVNGENSPEADSKKTDVTVILDYVANTNHTGMYVALDKGYYEEAGLNVNIVEPTEGAT
                     ATLVAVGKGDFGISYQEDVTVALASKDALPIKAIAAIIQHNTSGFVTYADKDIHSPKD
                     FEGKTYAGWGGPGEEAVLKAVMTQAGADPSKLNRIISDGSGFEALKDKVDIEWFFEGW
                     DNVKCKLNDFPINYMELRQLDERLDYYTPVIIANNDTLENRPELVKSFLAATEKGYKY
                     AIENPDESAQILHKYAPDYSMDLLKTSQEYLAGKYMEDTDRWGEMKDSVWDNYTDFMT
                     EYGVIDHAIPAADCYTNEFLPE"
     gene            4940..5686
                     /locus_tag="ACZPCL_03640"
     CDS             4940..5686
                     /locus_tag="ACZPCL_03640"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1161614.1"
                     /translation="MTKKLEVSDLVFDYENRKILNHLDFSVNEGEFVSILGPSGCGKS
                     TLLKLLTGVLKAEKGHILVDGQEIKGVTEHFAYMPQNDLLFPWKTILENVCLYGRIHG
                     SADGMKEQARKQMEAFGLKGYEDEYPSSLSGGMRQRAAFLRTLLCHADILLLDEPFGA
                     LDVITRGEMQDWLLSVRKQMNRTVLLVTHDMDEAIYLSDRILVLDAKKGKVRQEFTVK
                     EEKRNREWLYEQGALRREIYKEIMEGKVNV"
     gene            5683..6492
                     /locus_tag="ACZPCL_03645"
     CDS             5683..6492
                     /locus_tag="ACZPCL_03645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR1161615.1"
                     /translation="MIQLTDAHAHIKNEKQAMERITLGIPTMACAGTPGEMQELEKFG
                     RLQGAEKILIPACGLHPWYSDKWEPEEMFSLMEKVPVIGEIGMDSVWCDVPLDRQRKA
                     LEKQLQFACEIKKPVVLHTKGQEKEIARIISHYPNTYLVHWFSGDTGLEDYLKLDCYF
                     SIGPDVLWNPAVQKVAEQMPENRILIETDGMEAVDWAYEEGKKQGFLKEKKENMMPET
                     SLFETLKTTAGLRNCEPEALAEAVYANFYRFISVHMVVWRSDKLLKTGLVN"
     gene            6627..7172
                     /locus_tag="ACZPCL_03650"
     CDS             6627..7172
                     /locus_tag="ACZPCL_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1161616.1"
                     /translation="MSMNTKQAAAAAKSGYSTKELVLDAMFIALTYVFTAFVNVRLPI
                     AANGGLIHLGNVPLFICAILLGKRTGMLAGAFGMGLFDLLSGWTAWAPFTFVIVGAMG
                     YVVGAMTEGHRSTARDAAAIAVACVIKVVGYYIAEVILYHNLFAPVASIPGNLVQIGT
                     AAVIVLICVEQLRKVLRPILK"
     gene            7204..8337
                     /locus_tag="ACZPCL_03655"
     CDS             7204..8337
                     /locus_tag="ACZPCL_03655"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR1161617.1"
                     /translation="MYDIMTPGPTQVRENVRQARALACTNPDLDADFYDFYKETCEEI
                     SELLYTKNETLILDGEGILGLEAACATLTEKGDRVLVMDNGEYGKGFAGFVTMYGGEP
                     VLYSTDYRNAFDVKALEEYLEKDHDFKYAALVHCDTPSGMLNDVSKLCPLLKKYGILT
                     LVDSVSAMFGEEMRVDDYRIDLLCGGSQKAVSAPPGLSFVTISDDAYKAMRSRKTPVA
                     SFYANILAFDGYYEKQWFPYTMPISDIYGLRQAFDNIKNDPGMLERHAKIGEAVRGAV
                     VEAGLELYQETGFSNTVTVFNVPEGTTDTAILTAMKEKCNIMLAGSFGAFAGKVIRIG
                     HMGENAYPEKVEAVMAGLDRVFADLGITLKCSLKEAYDKRMNG"
     gene            complement(8371..8568)
                     /locus_tag="ACZPCL_03660"
     CDS             complement(8371..8568)
                     /locus_tag="ACZPCL_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HIR1161618.1"
                     /translation="MKNTALDATALTLSIIGAVNWGLIGLFKFDLVAFLFGSMTLLSR
                     IIYTIVGICGIYLISFYAKKE"
     gene            8789..10156
                     /locus_tag="ACZPCL_03665"
     CDS             8789..10156
                     /locus_tag="ACZPCL_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1161619.1"
                     /translation="MEQDMTRGNPLSVIFMFTLPLAVGNIFQQLYNMADTIIVGRFVG
                     ADALAAVGSTGTIMFLLNGFAQGITAGFSVLTAQRFGAGKKEGVKESVTNGVLLSIIG
                     AILFTVLSCGIMNPLLHLMNTPDDIFDKAYTYIILISLGMIANVFYNLLSSYLRAVGN
                     SKAPLFFLVFSACLNVGLDLFFIVVLKTGVAGAAWATNLSQGVSAVLCAGYIFLKVPA
                     LVPEKKHWRLTRRDTRYQLQMGIPMALQFAITSSGTMIMQSAINLFGSEAVAAYTAAC
                     KVHSLLTQGMVAMGQTMAVYSGQNYGKGDAKRIRDGVKAALLIDVIYSVVSAVLVSLL
                     LGVTLGLFFSGDVDLTVMMPWARTYITICTLFYIPLSTIFIFRNTMQGCGYGFLPMMG
                     GVVELVARLSVAMIAMRVHSYALSCFCDPAAWLSAGLFTGVSYLFVMKKIRASLEPKS
                     VTVQP"
     gene            10251..11081
                     /locus_tag="ACZPCL_03670"
     CDS             10251..11081
                     /locus_tag="ACZPCL_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024803.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1161620.1"
                     /translation="MDLSIGQMARINHISIQTLRLYDKMGLLKPVAVDAGSGYRYYSI
                     LQCAKLDLIGSLKQCGLSLKEIGSYFENGNIESLEGELRNTLDQIGTEMRRLSEQKEA
                     VKRTLESFEQYRVAPPAGTITLEYIPRRSIIVLDSGNNYFEEGLEFYEHSLLQLKHAL
                     ADRNVEHIYYRNPGTIWRKDKVMKREFVSTEVYVRADAERFRPDEITEIPAGMYQCIY
                     CSSFDTEKELACKLLDQVEKNGYTIAGDYLCETIVETPIFEKDERGMFLRLQVPVNVR
                     "
     gene            11227..12267
                     /locus_tag="ACZPCL_03675"
     CDS             11227..12267
                     /locus_tag="ACZPCL_03675"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent amine dehydrogenase family
                     protein"
                     /protein_id="HIR1161621.1"
                     /translation="MNKERIRVVQYGCGKMSKYTLRYLYEKGADIVGAIDVNPAVVGM
                     DVGQFCGLGFDLGVKIRNDADAVLDECQPDIAIVTLFSFVDDCYEHYAKCLSRGINVI
                     TTCEEATYPWTTSAACTNKLDVLAKENGCTIVGAGMEDIFWVHLIGTVAGGCHDIKKI
                     EGAVSYNVEDYGLALAKAHGCNLTPEEFEKTIAHPEVLEPSYVWNSNEALCNLMGWTI
                     KSQTQKCVPYFSDKEVYSETMGLTIPVGRCIGMSAVVTTETFQGPVIETQCIGKVYGP
                     DDGDMCDWKIKGEPDVTFYVQKPDTPVHTCATIVNRIPDVIMAPPGFITVDKLDSVEY
                     RTYPMHLYVDEY"
     gene            12482..13459
                     /locus_tag="ACZPCL_03680"
     CDS             12482..13459
                     /locus_tag="ACZPCL_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009535591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR1161622.1"
                     /translation="MWFWLALIALLCWSGSDLFSKIGCRGNDKYAHLKMVIAVGIIMG
                     IHALYEIFVGGVEVTWHVILTYLPVSLLYISSMTLGYIGLRYIELSISSPICNSSGAL
                     VAVLCFITGTLDDGIEGSMRLAVMGAVALVCIGVIGLGVVESREDDELRAERQKASNY
                     KYAKSWLALALPVAYCLLDAAGTFADTLVLDTLAGEVEAAGLFATAEECSSYAASVSN
                     CAYELTFLFAAVCCIVYVGFIKKQKFAFSQEGPKYFGAICETAGQFAYIFALSDTAHA
                     AISAPIISAYCVASVVWSRIFLKEKLSWKHYAMILLVIIGIVMLGVFDI"
     gene            13569..14189
                     /locus_tag="ACZPCL_03685"
     CDS             13569..14189
                     /locus_tag="ACZPCL_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HIR1161623.1"
                     /translation="MLQDKWIEFAVELQSLAQAGLAYGKDVYDLERYTRIREIAAEMI
                     ACKSDIPLEKVKNLFCNETGYQTPKVDTRAAVFKEGKILLVHEKNGTWSLPGGWCDVN
                     QSVAENTVKETLEEAGLNVKPVRMIAVQDRNKHNDPPYAYGIVKVFILCEVISGAFTE
                     NIETTEIRYFGKEEIPENLALEKTNKEQIQMCFKANEAKHWETQFD"
     gene            14220..14924
                     /locus_tag="ACZPCL_03690"
     CDS             14220..14924
                     /locus_tag="ACZPCL_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009861031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR1161624.1"
                     /translation="MTDIQKRLFELQDHAYGDFHSGLIPELPREYFIGVRVPVLKKFV
                     KEYKKEAESETFLKQLPHTYYEEYMVHILLLGEIKDYDTCMKVVEEFLPYIDNWAICD
                     GLSPKVFKNHKPELLEKIKQWIPSEHTYICRFGIEMLMRWFLDEDFKPEYLKMPASVR
                     SDEYYVNMMIAWFFATALAKQWDATIPYLQDPVLEPWTHNKTIQKARESFRITPEQKE
                     YLKTLKVGCKKSIKRP"
     gene            15017..15922
                     /locus_tag="ACZPCL_03695"
     CDS             15017..15922
                     /locus_tag="ACZPCL_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1161625.1"
                     /translation="MTLQQLTYIKMTAECGNITEAAEKLFISQPSLSTAIHNLEKEMG
                     VTIFVRSKKGVVLTREGEELLSYAGMLLEQVDIMKEHFGVGKARMPKFSVSCQHYSFA
                     VNAFVDVVQTYDAAKYDFILRETQTGEIIDDVANGKSELGVIYLSEHNEEVLTKLIKK
                     NGLIFEELFTAEPHVFISNVHPLAVKAKIQLEDLKAYPYLTYEQGERNSFYYAEEFLS
                     MLDMPKNIQVRDRATMFNLIIGLNGFTVCSGVISEELNGSQIIARPLDMDVQMRIGLV
                     KKKNILFSRYADTYIEALKKYVSID"
     gene            16061..17182
                     /locus_tag="ACZPCL_03700"
     CDS             16061..17182
                     /locus_tag="ACZPCL_03700"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HIR1161626.1"
                     /translation="MADLKTPFRYDYVGSFLRPEALKQARKNFEEGKITKEELTAVED
                     DCIRDLVGKIKKLGYHVITDGEFRRSTWHLDFMWGFQGIEHRKTVEGNTTFDAEAAMI
                     DDTYMVGKISVKNHPFVEHFKFVKALEDENTVAKQTIPSPGQFYAYFTGAELLGTTLD
                     IYGSEEAFAKDVAGAYVEFVNEIYAAGCRNLQFDDCVWGGMVNPKLAVALTGRKGDAL
                     EAYKKLLLQLNNEVAAQAPADLVINTHVCRGNYHSTFFSSGAYDGVADLLFGEENVNA
                     YYLEYDDERSGGFAPLAKVSGDKKVVLGLVTTKSPVLEKKEDVIARIHEAAKHVPLDR
                     LYLSPQCGFASCEIGNKLTEEEQWAKLKLVKEIAEEVWR"
     gene            17408..18934
                     /locus_tag="ACZPCL_03705"
     CDS             17408..18934
                     /locus_tag="ACZPCL_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR1161627.1"
                     /translation="MGSDKIYIAIDLKSFYASVEAVDLGLDPLTANLVVADPSRTEKT
                     ICLAVSPSLKAYGISGRARLFEVVQKVKQANSLRRSGLPQRKFTGRSCDAKMLERDPR
                     LEIDYVIAPPQMAHYMEVSTKIYHIYLKYVAPEDIHVYSIDEVFMDVTEYLGTYRMTA
                     RELAMKMIQDVLSETKITATAGIAPNLYLCKVAMDIVAKHVAADQDGVRIAELDEMSY
                     RQMLWDHRPLTDFWRVGKGYARKLEENGIYTMGDIDRCSLGRSGDFYNEDLLFRLFGI
                     NAELLIDHAWGYEPCTIADIKAYKPQDKSIGSGQVLHCPCDFNQAKLVLKEMADMLAM
                     DLVDKGLIASQIVLTVGYDIENLTDPVRRKAYKGAVTTDQYGRKIPKHAHGTIHLKLP
                     TSSARLIMQGAMELYDGIVDKNLLIRRLYITAEHAMEEKKVQKQETYEQLSLFTDYEA
                     VEKEREKEVKNLEKEKRLQQAMLDIKKKYGKNAILKGMNLEEGATAIERNRQIGGHKA
                     "
     gene            18991..19326
                     /locus_tag="ACZPCL_03710"
     CDS             18991..19326
                     /locus_tag="ACZPCL_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HIR1161628.1"
                     /translation="MSRENRAAQFAPFAALTGFEGAIKETARVTGEKIELDETQKTFL
                     DEKLKVLLREKTPALFTYFQKDEQKEGGAYITVSGIVKKTDAYTHKIVLEDGTGICVE
                     DILEIRKLL"
     gene            complement(19333..20475)
                     /locus_tag="ACZPCL_03715"
     CDS             complement(19333..20475)
                     /locus_tag="ACZPCL_03715"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88/105 protein"
                     /protein_id="HIR1161629.1"
                     /translation="MKILDRYIDQLLEKSSPSRPIWNIEKIRQGAKPSWNYMDGCMIN
                     AILELYHITQKEEYLDFADRFIDYFVEEDGSIHSYDPKEHNLDNVNTGKTLFDLYDLT
                     GKEKYRLAIDLVYSQLKTQPRTSTGNFWHKEIYPNQIWLDGLYMAQPFYMQYEVTYNN
                     STNCKDCYQQFVNVYNLMRDLRNGLYYHAYDDSRTSFWCDKVTGLSDNFWLRALGWYA
                     MALIDTMEVMPEETLGSEKKEMNRIYKELIDSMLPYQDEETGMWYQVVNRGGIHPNYL
                     ETSGSAIFAYAIMKSVRLGFLDGSYFAYGKKAFEGICRTYLSEENGELQLGGICLVAG
                     LGNKEMREGTFDYYMREPIVKNEAKGVAPLILAYIELMYQHQEAEV"
BASE COUNT         6128 a         3889 c         5180 g         5369 t
ORIGIN      
        1 tttatattta ttagagcaga aaatgcccat agatttatta atattgcctg ctgggggaaa
       61 agggggagag atctacggtc aattggaaca gctgcagaca gaagcagggg gaaaaacata
      121 ttacatgcac cagggagata agataaaggc cggagaaatg gaatttacct gtatttttga
      181 gaaggagaca gaagaagaac gtaacgccca ttccctggtt ttatgctccc actataaaga
      241 tctgcatatt ttatttaccg gtgatatggg aatatcagaa gagacagaac ttttagatct
      301 ggcagaagaa aatggcagca tccagcagga acatttggaa catgtacaga tcctgaaaac
      361 ggcccaccac ggttccaaag gatcttccag tcctgccttt ttggaagcaa tgcctttaaa
      421 agctgctttt atttcctacg gtaaagacaa ttcctatgga catccatccg ggcaggtcac
      481 ggaggagatg aagaaacaaa acatttccct ttatgaaacc ggcggaaagg gggcatggac
      541 attagaaagt aaagcgagaa acgttattat aaaaagaacc gtgaaaagca ggggagaaaa
      601 ttaaaataaa aggttttcaa gtaagaaaag aagtggtatt gttattaaca aaatttgcag
      661 ccccatatgc cagggacttg aatacaggag gccagaataa tgagtgagaa aaatgaaaat
      721 gtaaagatcc aggtggcatc agtggaagat gcagaggagc tgcttgcaat ttatgctcct
      781 tatgtagaaa agacagcgat cacttttgag tatgaggttc cgtccgtaga agaatttaaa
      841 ggacggattg cccatgtatt acagcgtttc ccatatctga aagcagtgga agatggtaag
      901 atccttggat atgcttatgt aagtcctttt aaagagcggg ctgcttacgg ctggtcagtg
      961 gaaacatcta tttatgtaga tgaaaatgtg aagcacaaag gggttggaag aaagcttcat
     1021 gaggtcctgg aagctgtctt aaaagagcag ggcatcctga atatggaagc ctgcatcggt
     1081 tatccggaag tggaagacga atacctggat aaaaacagtg tcaatttcca cgcacacatg
     1141 ggataccgca tgataggcga gttcaaaaaa tgtggataca aatttgaccg ctggtacaac
     1201 atggtatgga tggagtacat cattggcgag catcaggccc atatgacagc accggaaagc
     1261 ttcccgcaga tccgggacag actgaaagaa aaatatggtt tagagtagag ttgtaaatgc
     1321 gttgtaagcg tccttcgggg cgcttttgct ttgtctgggg aaattttatt taaagcagtt
     1381 tttggaaaaa agaagtgttg ttgatacaat aaatccgtgg taaacttgga atgagttttt
     1441 tgaaaatggt agtaaatatt taactgcgag gagaaaaaaa tgccagtttt tgattttaat
     1501 gatgaaccga aagaagaaaa taaagcaccg gaatgcacct acactgtgat ccgttccaat
     1561 gaaacaaccg cttctccaga acatccgatc ctggttctgg acaacagtaa tagaaaatgg
     1621 gagcaccatt cctggggtgt atttacaaat cccatccgta aaactgcttt tgaatttcag
     1681 gtagaaggcg gcggcactgt cagcgctgat atcttaaaag tagatgcccg ctttgtaagc
     1741 ctgcttaaat ggctgggcga gagccatatc aatgtacgcc tttccggctc caatgaggcg
     1801 gacgggtatg ctgtatacaa gatccgtgag atcgcttttg gcggcggcac caagctttcc
     1861 gcagaggacg gatttttaca gtacatgatc gagcgccttc aggccagcag tgcgcctgca
     1921 gaggagatcc gggaagagga tgcagatgaa gacggcgatg atatgaagct gaccagttta
     1981 cagagtatta ccgactttat gacctgcgcg ggaaggaccc ttcctgacaa tatccgtctt
     2041 tgggcaagaa gaaacctggc agttgccaat tcccatgagg tttcaccgga agaacgccgt
     2101 cacgcacaga gagctttatc cattatgatg aacatccagt ggaagagcaa ttattttgag
     2161 gccattgacc cagacgaggc aagacggatc ctggatgaag aattatacgg aatggaacgt
     2221 gtaaaacagc gtatcatcga gaccattatc cagatcaacc gtactcatac actgcctgct
     2281 tacggtatcc ttttgatcgg acctgcagga actggaaagt cccagatcgc ctatgcagta
     2341 gcaaggatcc tgaagctgcc gtggactact ctggatatga gttctattaa tgatccggaa
     2401 cagctgacag gaagctcccg tgtatatgca aacgcaaaac caggtatcat catggaggca
     2461 ttttctctgt ccggtcagtc caatcttgta tttattatca atgagttaga taaggctgct
     2521 tccggcaaag gaaatggaaa tccggcagat gtgcttttaa cattgcttga caatcttgga
     2581 tttacagata actacatgga gtgtatggtt cctactgcag gtgtatatcc gatcgcaaca
     2641 gccaatgacc gttctcttat cagcgctccg ttaatgtcac gttttgcagt gatcgatatt
     2701 ccggattata cagaagatga aaagaagatc attttcacaa aatttgccct tccaaagata
     2761 ttaaagagaa tgagcatgaa ggaagatgaa tgtgtgatcc ttccggaagc tttagatgag
     2821 gtagtccgcc actattctga tactaccggt atccgtgatc tggagcaggc agcagagcac
     2881 attgcagcca atgccctgta tcagatcgaa gtaaatcatg tggatcaggt tgtatttgac
     2941 gcaaaaatgg ttcgggaact gttaggttaa gggacaactc acatgaaaca tgtaaaagat
     3001 aaaatgatac cggcagtgct tattctggtg ctgctggtga tctgggaggc agcagtggac
     3061 ctgctgcaca tccctttata tgtacttcca tcacctctgg aagtggtaaa agccctgttc
     3121 acggaaggaa cagagctttt tagccatggc ctgactacag taggagaggg acttttggga
     3181 attttgatcg cgtcagcttt gtcactggtg cttggaattt ccatggactg gttcccggct
     3241 gtgcgaaagg gactctatcc tattctggta gtgacccaga cggttcccat gatcgttatg
     3301 gctccgatcc tgatcattta tatgggattt ggaatggcac cgaagatcct tacggtagtt
     3361 cttatgtgtt tctttccagt ggcagtcagt tttgcagacg gactgcagcg ggtagacgaa
     3421 gaatatgtac atctggtgcg ctcctatggg gcagaccggt ggaaagctta ttgcctggta
     3481 aagataccgg cagcgctgcc ggaactgttt tccggactga aagtggcagc gacctacagc
     3541 atcagcggtg ctgtagtagg agagtggctt ggttcccaga agggacttgg ctattacctg
     3601 ttaagggtaa agaacagcta tatgctggat aaggtgtttg cctgcgtggt ggtcattatc
     3661 ctgttaagtc tggctatgaa cgggctgatc cgggtgatcg ctaaggtgtc tatgccttat
     3721 gagagaaata agagatcgtg agtttcccgc cgcaggcggg gagcaggtgt accatttcct
     3781 gcaatcaaca gagaaacaga cagaaaatga gaggaaatat tatgaaaaaa tcttggaaaa
     3841 aagccagtgc tatttttatg gcagcagctc ttgcagccgg acttgcaggc tgcggcgcgt
     3901 ctaacaataa caatacagct acaacagctg caggcaaaag cgcagcagaa agcaaagtaa
     3961 atggtgaaaa cagcccagag gcagatagca aaaaaacaga tgtgactgtt atcttagatt
     4021 atgttgcaaa caccaaccac accggtatgt atgtggctct ggacaaaggc tattatgagg
     4081 aagctggact taatgtaaac attgtagagc cgacagaagg cgctactgcc acactggttg
     4141 cagtaggaaa aggtgatttt ggcatcagtt atcaggaaga tgtaaccgtt gccttagcgt
     4201 ccaaagacgc ccttccgatc aaggccatcg cagccatcat ccagcacaat acctccggtt
     4261 ttgtaactta tgcagacaag gatatccact ctccaaagga ttttgaggga aagacctatg
     4321 caggctgggg cggacctggt gaagaggctg ttttaaaagc tgttatgaca caggcaggtg
     4381 cggatccttc caaactgaac cgtattatct ctgacggttc cggttttgaa gcactgaaag
     4441 acaaagtaga tattgagtgg ttttttgaag gctgggataa tgtaaagtgc aagctgaatg
     4501 atttcccgat caactacatg gagctgcgcc agttagatga gcgtctggat tactatactc
     4561 cggtgatcat tgcaaataat gatacgttag aaaaccgccc ggaactggta aaaagcttcc
     4621 tggcagcaac agaaaaaggc tataaatatg ccattgaaaa cccggacgaa agtgcccaga
     4681 tccttcacaa atatgccccg gactattcta tggatctgtt aaaaacatcc caggaatatc
     4741 tggctggaaa gtatatggaa gataccgacc gctggggtga gatgaaggac agcgtatggg
     4801 ataactatac ggactttatg acagaatacg gcgtcattga ccatgccatt cctgctgcag
     4861 actgctatac caacgaattt cttccagaat aaaagcgtat tacttataaa agagtatcac
     4921 ttataaagga gtgccactta tgacaaaaaa gctggaggtc tcagaccttg tatttgacta
     4981 tgaaaaccgg aaaatactga accacctgga tttttctgta aatgaagggg agtttgtcag
     5041 tatcctggga ccttccggct gcggtaaatc cactctttta aagctgctta ccggagtttt
     5101 aaaggcagag aaaggtcata ttttagtgga cggacaggag ataaaaggcg ttacagagca
     5161 ttttgcctat atgccacaga atgatctttt attcccctgg aagaccattt tagaaaatgt
     5221 gtgcctttat ggccggatcc atggaagcgc tgatgggatg aaggaacagg caagaaagca
     5281 gatggaagcc tttggtttaa agggttatga agatgaatat ccatcttcct tatccggcgg
     5341 aatgcgccag agagcagcat ttttacgcac ccttttgtgc catgcggata tcctgctttt
     5401 agatgagcct tttggcgctt tggatgtgat taccagagga gagatgcagg actggctttt
     5461 aagtgtgcga aaacagatga accgtacagt tcttcttgtg acccacgata tggatgaggc
     5521 catctatctt tccgaccgta ttctggtact ggatgcgaaa aaaggcaagg tacggcagga
     5581 atttacagta aaagaagaaa aacgaaacag ggagtggctg tatgaacagg gcgctctgcg
     5641 cagggagatc tataaagaga tcatggaagg taaggtaaat gtatgataca gcttacagat
     5701 gcccatgcac atataaagaa tgaaaaacag gccatggaac ggataacctt agggatccct
     5761 accatggcct gtgccggtac accgggagaa atgcaagaac tggaaaaatt tggaaggctg
     5821 cagggagcag aaaagatcct gatccctgcc tgcggccttc acccatggta cagtgacaaa
     5881 tgggaaccgg aagagatgtt ttccttaatg gaaaaagtgc ctgtaatagg cgagatcgga
     5941 atggacagtg tgtggtgtga tgtgccactt gaccggcaga ggaaggcttt ggaaaaacag
     6001 ctgcagtttg cctgtgagat caaaaagccg gtagtccttc acaccaaagg ccaggaaaag
     6061 gagatcgccc gtatcatatc ccattatcca aacacctatc tggtccactg gttttccggg
     6121 gatacaggtc ttgaagatta tctgaaactg gactgctatt tttccattgg accggatgtt
     6181 ttgtggaacc cggcagtaca gaaggtggca gagcagatgc cggaaaacag gatccttata
     6241 gaaacagatg gaatggaggc tgtggactgg gcttatgaag aaggaaagaa acagggattt
     6301 cttaaagaga aaaaagaaaa catgatgccg gaaacttctc tgtttgagac tctgaaaaca
     6361 acggcaggac ttagaaactg tgaaccggaa gcgctggcag aggcggtgta cgccaatttt
     6421 tatcgtttta tatccgttca tatggtcgta tggcgcagtg ataaattact aaaaactgga
     6481 ctggttaatt aattgaaaac tggatgtttg aaaaaaacca attatcattt atccttgtgt
     6541 taagctaata aagcaaatac aaaaagaaag cctgatgcta ttctccctgg gccccgagaa
     6601 cccgtcagga agagaggaaa aggatgatga gtatgaacac aaaacaggca gctgcggctg
     6661 caaaaagcgg atacagcaca aaagaacttg ttttagacgc aatgtttatt gcactgacct
     6721 atgtatttac agcatttgta aatgttagac tgcctatcgc tgccaatggt ggactgatcc
     6781 atttgggaaa tgtgccttta tttatctgtg ccattctatt gggcaagcgc accggtatgt
     6841 tagcaggcgc tttcggtatg ggactgtttg atctgttatc cggctggact gcatgggcac
     6901 catttacatt tgtgatcgta ggtgctatgg gttatgtagt tggtgctatg acagaaggac
     6961 accgcagcac agcaagagat gcggcagcga tcgctgtagc ctgtgtgatc aaagtagttg
     7021 gttactatat tgcagaagtg atcctgtatc acaacctgtt tgctccggtt gcttctattc
     7081 ctggaaacct ggtgcagatc ggtacagcag ctgtgatcgt tctgatctgt gtagagcagc
     7141 ttcgcaaggt acttcgtcct attttaaaat aagacagaaa aataagagaa aggaaccatg
     7201 atcatgtacg atatcatgac acccggccca acacaggtaa gggaaaatgt acgtcaggcg
     7261 agagcgcttg cctgcaccaa tccagatctg gatgcagatt tttatgattt ttataaggag
     7321 acctgtgaag agatcagcga actcctttat acaaaaaatg agaccctgat cctggacgga
     7381 gaaggcattt tgggactgga agcagcctgt gccactctca cggaaaaagg agaccgggtg
     7441 ttagtaatgg acaatggaga atatggaaaa ggtttcgccg gtttcgtgac tatgtacggc
     7501 ggagaaccag tcctgtattc cacagattac cgcaatgctt ttgatgtaaa agcgctggaa
     7561 gaatatctgg aaaaggatca cgattttaag tatgcagcct tagtacactg tgacactcca
     7621 agcggtatgt taaatgatgt ttcaaagctt tgtccattat taaagaaata tggtatcctg
     7681 accctggtag actccgtttc cgccatgttc ggtgaagaaa tgcgtgtgga tgattaccgg
     7741 atcgatcttc tgtgcggcgg ttcccagaag gcagtttccg cacctccagg attaagtttt
     7801 gttaccatca gtgatgatgc ttataaggca atgcgttcca gaaaaacacc ggttgcgtct
     7861 ttctatgcaa atatcctggc atttgacggc tactatgaga agcagtggtt cccatatact
     7921 atgccgatca gtgatatcta tggtctgcgt caggcctttg ataatattaa aaatgatccg
     7981 ggcatgttag agcgccacgc aaagatcggt gaagcagtaa gaggagcggt agtggaagca
     8041 ggactggaat tataccagga aaccggtttt tccaatacag tcactgtatt taatgttccg
     8101 gaaggaacca cagatacagc catccttact gcaatgaagg aaaaatgcaa cattatgtta
     8161 gcaggttctt tcggtgcttt tgcaggaaag gtgatccgta tcggtcatat gggtgaaaat
     8221 gcatatcctg aaaaagtaga agctgtgatg gccggactgg acagagtatt tgcagatctg
     8281 ggaattacat taaaatgcag cctgaaagag gcttatgata agagaatgaa tggataatgg
     8341 tttaaaagaa cggggggctg agatgccccc ttattctttt ttggcataaa agctgatgag
     8401 ataaataccg catatgccta ctatggtata gatgatccgt gacagcaatg tcatgcttcc
     8461 aaaaagaaat gcaacaaggt caaacttaaa aaggccgatc agcccccagt ttacagctcc
     8521 gataatggat aaggtaagag ctgtagcgtc cagtgccgta tttttcatgg cagtttacct
     8581 ccttatacat agccagtatg gaccggagac agttaaaata tacatgatct tatgggatca
     8641 ttgaaaaatc acctgccaaa aaatctgtga aagagtaaaa aattttcaat gacaattaac
     8701 ggacagtgtg ctatgctatg ttgcgcggaa cctgagtccg cgtttattta tgatctgttt
     8761 tgatataaga taagaaaggt agaaaattat ggaacaggat atgaccaggg gcaatccact
     8821 gtcagttatt tttatgttta cattgcctct tgcagttgga aatattttcc agcagcttta
     8881 taatatggcg gatacgatca tcgtaggccg ctttgtggga gcagatgcct tagcggcagt
     8941 tggctccaca ggaaccatta tgtttttgct caacggtttt gcacagggca ttacagccgg
     9001 cttttccgtt cttacggccc agcggtttgg tgcgggaaaa aaggaagggg taaaagaaag
     9061 cgtgaccaac ggcgttcttt tatctattat aggtgccatt ctttttaccg ttttaagctg
     9121 tgggatcatg aacccgctgc tgcatctgat gaatacgccg gatgatattt ttgataaggc
     9181 atatacctat atcattttga tcagcctggg aatgatcgcc aatgtatttt ataacttatt
     9241 gtcctcttat ttaagagcag ttggaaacag caaggcacct ttatttttcc ttgttttttc
     9301 agcctgctta aatgtaggtc tggatctgtt ttttattgta gtcctgaaaa cgggagtggc
     9361 gggagcagcc tgggctacca atctgtcaca gggagtatcg gctgttttgt gtgcaggata
     9421 catttttttg aaagtgcctg ccctggtgcc ggagaaaaag cactggcgtc ttaccagaag
     9481 agatacccgc tatcagctgc agatgggtat tcccatggct cttcagtttg caattacttc
     9541 ttccggaacc atgatcatgc agtcagctat caatcttttc ggttcagagg cagtagccgc
     9601 ctatactgca gcctgcaagg tacatagttt gctgacacag ggaatggtgg ctatgggaca
     9661 gaccatggca gtttacagcg gccagaacta cggaaaagga gatgcaaaga ggatccggga
     9721 cggtgttaag gcagcattgc tgatcgatgt gatctactct gttgtatcag ccgtgctggt
     9781 atccctgctc ttaggtgtga cactgggcct gttcttctca ggagatgtgg accttacggt
     9841 tatgatgccc tgggcaagga cttatattac catctgtaca ttgttttata ttcctcttag
     9901 tacgatattt attttcagaa ataccatgca gggatgcggc tatggtttcc ttccaatgat
     9961 gggaggtgta gtggaactgg tagccagatt gagtgtggct atgatcgcca tgcgtgttca
    10021 cagctatgcg ttgtcctgtt tctgcgatcc ggctgcatgg ctcagtgcag gtttgtttac
    10081 aggtgtgtct tatctttttg taatgaagaa gatcagggca tcactggaac cgaagtcagt
    10141 tacggttcag ccatgacaga acggtaaaac tctatagtaa gtatagatag gtatagacaa
    10201 aaactgaggc atttttacaa aaaaatttgg ggtgtacggg ggtgaaaatt atggatctga
    10261 gtattggtca gatggccagg atcaaccaca tcagcatcca gactttaaga ctgtatgata
    10321 aaatggggct gctaaagccg gtagcagtag atgcaggatc cggatacagg tattacagta
    10381 tccttcagtg tgccaagctg gatctgatag gcagcctgaa gcagtgcggc ctcagcctga
    10441 aagagatcgg gtcatacttt gaaaacggga atattgaaag cctggagggg gaacttcgca
    10501 ataccttaga ccagatcggt acagagatga gacggctgtc agaacagaaa gaggcagtaa
    10561 aacgcacgct ggagtctttt gaacagtacc gtgtggctcc gcctgcaggt accattaccc
    10621 tggaatatat ccccagacgc agtattatcg tgctggacag cgggaataat tactttgaag
    10681 aagggctgga attttatgaa cacagccttt tgcagttaaa acatgctctg gcagatcgga
    10741 atgtggagca tatttattac cgcaatccag ggaccatctg gcgcaaagat aaggtgatga
    10801 agcgggaatt tgtttccacg gaagtttatg tccgggcaga tgcggagagg ttccgtcctg
    10861 atgagattac agagatcccg gcgggaatgt atcaatgcat ttactgcagc agttttgata
    10921 cagaaaagga gctggcatgt aagcttcttg atcaggtgga aaagaacgga tataccattg
    10981 caggggatta tctctgtgag actattgtgg aaacacctat ttttgagaag gatgaaagag
    11041 gaatgttttt gcggcttcag gtgcctgtaa atgtgaggta agagaaaaaa ttgtcctaaa
    11101 tctgcccttg actctctact tagtataggc tctataatga aagtacaaag aaggttaatt
    11161 aattaacaag caaatcaatg ttgccaatgt tattattcat gtacatcatt acaaggaggt
    11221 tacactatga ataaagagag aatcagagtg gttcagtacg gatgcggaaa gatgtcaaaa
    11281 tacacactgc gttatcttta tgagaaaggc gcagatattg taggcgcgat cgatgtaaat
    11341 cctgctgtag taggcatgga cgttggacag ttctgcggac tgggatttga tctgggcgta
    11401 aagatccgta acgatgcaga cgcagtattg gatgagtgcc agccggacat tgccattgta
    11461 accttattca gttttgtgga tgactgctat gagcattatg caaaatgttt aagcagaggc
    11521 atcaatgtaa ttaccacctg cgaggaagca acctatccat ggaccacttc cgcagcatgc
    11581 accaataaat tagacgttct tgcaaaagaa aacggctgta ccatcgttgg agcaggtatg
    11641 gaagatatct tctgggtaca tctgatcgga accgtagcag gcggctgcca tgatattaag
    11701 aagatcgaag gcgctgtaag ctacaatgta gaagattatg gtcttgcact tgcaaaagct
    11761 cacggctgca acctgacacc ggaagaattt gaaaagacca ttgctcatcc ggaagttctg
    11821 gaaccatctt atgtatggaa ctccaacgaa gcgctctgca acctgatggg atggaccatt
    11881 aagagccaga cccagaagtg cgttccatat ttctccgata aagaagttta ttcagagacc
    11941 atgggactga ccattccggt aggacgctgc attggtatga gcgcagtggt tacgaccgag
    12001 actttccagg gaccagtgat cgagacacag tgtatcggta aagtttacgg accagatgac
    12061 ggcgatatgt gtgactggaa gatcaagggc gagccggatg ttaccttcta tgtacagaaa
    12121 ccggatacac ctgtccacac ctgcgcaacc atcgtaaacc gtatcccgga tgtaattatg
    12181 gcacctccag gatttatcac cgtagacaaa ttagacagcg tagaatacag aacttatcca
    12241 atgcatctgt atgtagatga atattaataa tagtaataca agtgttttta agcttaagcg
    12301 gccgcagtga tgcggccgtt tttctgcttc tatttggaaa tatctgggat taatgataaa
    12361 acttggcacc agaaagggtt gatcgccctt gcgaaaaagt aagttcggat atataataag
    12421 caagtctgtc cagatgggaa aaactggaag ataagaaaaa tgcaaaggaa tttagaaaaa
    12481 tatgtggttt tggcttgctc ttattgcgct cctgtgctgg agcggatccg acctgttttc
    12541 aaagattggc tgtcgcggca atgacaaata tgcccattta aaaatggtca ttgctgtagg
    12601 tattatcatg ggtatccatg cactgtacga gatcttcgta ggcggtgtgg aagttacctg
    12661 gcacgtaata cttacttatt taccggtatc cctgttatac atttcctcta tgacactggg
    12721 atatatcggc cttcgttaca ttgagttatc tatttcttca ccgatctgca actcttccgg
    12781 tgccctggta gcagttttat gctttatcac aggtacactg gatgatggaa ttgaaggttc
    12841 catgcgtctg gctgttatgg gcgctgttgc ccttgtatgt atcggcgtca tcggacttgg
    12901 cgtcgttgag tcaagagaag atgatgagct gcgtgcagaa cgtcagaaag catccaatta
    12961 caaatatgca aaatcctggc tggctctggc acttcccgtt gcgtactgcc tgttagatgc
    13021 agcaggtaca tttgcagata ccttagttct tgatacactg gctggtgaag tggaagcagc
    13081 aggattgttt gcaacagcag aggagtgctc ttcctatgca gcttctgtat ccaactgtgc
    13141 ttatgaactg actttccttt ttgcagctgt ctgctgtatc gtatatgtag gctttatcaa
    13201 aaagcagaag tttgcattca gccaggaagg accgaaatac ttcggtgcaa tctgcgagac
    13261 tgctggtcag tttgcttaca tttttgcatt atctgatact gcacatgctg ctatttctgc
    13321 gccgatcatt tccgcatact gcgtggcatc tgttgtctgg agccgcattt tcttaaagga
    13381 aaagctgtcc tggaagcatt atgcaatgat cctgctggtt atcattggta ttgtaatgct
    13441 tggtgtgttt gatatttaga ataaaacgag agatgtcttt ttacaattta catttcagat
    13501 caaaggctgc tgtagaaata cggcagcttt tttgatatga taaaaggagc agaaaagggg
    13561 aattatatat gcttcaggat aaatggatcg aatttgcagt agaacttcaa agccttgcac
    13621 aggcgggact ggcttatgga aaagatgtgt acgatctgga acggtatacc aggatacggg
    13681 agatcgcagc ggagatgatc gcatgcaaaa gcgatattcc gctggaaaaa gtaaaaaatc
    13741 tgttttgcaa cgaaacaggt tatcagactc caaaagtaga tacaagggcg gctgttttta
    13801 aagagggaaa gatccttctg gtccatgaga aaaatgggac ctggtcactg ccgggaggat
    13861 ggtgtgatgt aaatcagtcc gtggctgaaa atacagtgaa agaaacattg gaagaagcag
    13921 ggctgaatgt aaagccggtg cgtatgatcg cagttcagga caggaacaag cacaatgatc
    13981 cgccttatgc ttatgggatc gtgaaagtat ttattttgtg tgaagtgatc agtggggcat
    14041 ttacggaaaa tattgaaaca acagagatcc ggtattttgg taaagaggaa attcctgaaa
    14101 acctggctct ggaaaagacg aataaagaac agatccaaat gtgttttaag gcaaatgaag
    14161 cgaagcactg ggaaacgcag tttgattaaa ttattaaaat aaaaggataa aaagctgata
    14221 tgacggatat tcagaaacga ttatttgaat tacaggatca tgcatatggg gatttccaca
    14281 gcgggctgat cccggaactg ccaagagagt attttatcgg ggtccgtgtg ccggttctga
    14341 aaaaatttgt aaaagagtat aaaaaagagg cagagtcaga aacattttta aagcagctgc
    14401 ctcataccta ttatgaagaa tacatggtcc atatcctttt gctgggggag atcaaagact
    14461 atgatacctg tatgaaggta gtggaagaat ttcttcccta tattgacaac tgggctatct
    14521 gtgacggcct ttctcctaag gtatttaaaa accacaaacc ggaattgtta gaaaagatca
    14581 aacagtggat cccttctgag catacgtata tctgccggtt tggcattgaa atgctgatgc
    14641 gctggttctt agatgaagat tttaagccgg aatatctgaa aatgccggct tctgtccggt
    14701 cagatgaata ctatgtgaat atgatgatcg cctggttctt tgcaaccgct cttgcaaagc
    14761 agtgggatgc aaccattcct tatctgcagg atccggtttt agaaccgtgg acccacaata
    14821 agaccatcca gaaggcaagg gaaagtttcc gcattacgcc ggagcagaag gaatatttaa
    14881 agactctgaa agtagggtgc aaaaagagca tcaaacggcc ataaaacagt catcaaacag
    14941 tcatcaaaca gcgtaaaaat cctgaaagtt gggaaaggca gaaggaaaca taaaaaaggg
    15001 caaaaggagt acatctatga cactgcagca gctgacctac ataaaaatga cggccgaatg
    15061 tggaaatatc acagaagcgg cagaaaaact ctttatatcc cagccaagcc tcagcacagc
    15121 gatccataac ctggaaaaag aaatgggagt gactattttt gtcaggtcca aaaagggtgt
    15181 agtccttacc agggaagggg aagaactgct gtcttatgcg ggaatgctgt tggaacaggt
    15241 ggatattatg aaggagcatt tcggcgtcgg aaaagcgcgg atgcccaagt tttctgtttc
    15301 ctgccagcat tattcctttg cagtcaatgc ctttgtagat gtggtccaga cctatgatgc
    15361 ggcaaaatat gattttatcc tcagggaaac acagaccggt gagatcattg atgatgtggc
    15421 aaatggaaaa agtgagctgg gagtgatcta tctgtctgaa cacaatgaag aagtgctgac
    15481 gaagctgatc aaaaagaatg gcctgatctt tgaagaatta tttacagcag agccgcatgt
    15541 atttatcagc aatgtacatc ctctggcggt gaaagcgaag atacagttag aggatctgaa
    15601 ggcgtatcca tatcttactt acgaacaggg ggagcggaat tctttttatt atgcagaaga
    15661 gtttttaagc atgctggaca tgcctaaaaa cattcaggta agagacaggg ctaccatgtt
    15721 taatctgatc attggtttaa atggttttac tgtgtgttcc ggagtaataa gcgaggaact
    15781 aaatggctcc cagatcattg caagaccgct tgacatggat gttcagatgc ggatcggtct
    15841 ggtgaaaaag aaaaatatac tgttcagccg ttatgcagat acttatattg aagcactgaa
    15901 aaaatatgta tccatagatt gaaactatgg ataacataaa atataaagta ttatcccaaa
    15961 atgaaaacta ctgttaaact atcctcagaa aaaaacagac gggaaccaga taacaactgg
    16021 ttgattgatc tataaaattg tgaaaacgga ggataagaca gtggcagatt taaaaacacc
    16081 atttcgttat gattatgtag gaagcttctt aagaccggaa gcattaaagc aggcaagaaa
    16141 gaattttgaa gaaggaaaga tcacaaaaga agaactgaca gcagtagaag atgactgcat
    16201 ccgtgatctg gttggaaaga tcaaaaagct gggatatcat gtgatcacag acggtgagtt
    16261 ccgcagatcc acctggcatc tggattttat gtggggattt cagggcattg agcatagaaa
    16321 gacagtagaa ggaaatacga ctttcgatgc agaggcagct atgatcgacg atacctacat
    16381 ggtaggcaag atctctgtaa agaaccaccc gtttgtagag cattttaaat ttgtaaaagc
    16441 attagaagat gaaaatacag tagcaaagca gactattccg tctccaggac agttttatgc
    16501 ttactttaca ggtgccgagc ttcttggaac tacactggat atctatggaa gtgaagaagc
    16561 ttttgcaaaa gatgtggcag gggcatatgt ggaatttgtc aatgagatct acgcagcagg
    16621 ctgccgcaac cttcagtttg atgactgtgt atggggcggt atggttaatc caaagctggc
    16681 agttgcattg accggaagaa agggagatgc gttggaggca tacaagaaac tgctccttca
    16741 gttaaacaat gaagttgcag ctcaggcacc ggcggatctg gtgatcaata cccatgtatg
    16801 ccgcggaaac tatcattcca catttttcag ttccggtgca tacgacggag tagccgacct
    16861 gctttttggc gaggaaaatg taaatgccta ctatctggaa tacgatgatg aaagatccgg
    16921 cggttttgct cctttagcaa aagtttccgg cgacaaaaaa gtagttctgg gccttgtaac
    16981 caccaaatcc ccggtattag aaaagaaaga agacgtgatc gcaaggatcc atgaggctgc
    17041 aaaacacgtg cctttagacc gcttatactt aagcccacag tgcggtttcg cctcctgtga
    17101 gatcggaaac aagcttacag aagaagagca gtgggcaaag ttaaaattgg tgaaagaaat
    17161 tgctgaagag gtgtggagat agaaataagc aaatgacttt aatgatattc tgaaaattct
    17221 aatattcgct ggtgccgtat ctggtgtggc tgacttatgt gggatatctg aatttcatga
    17281 tcagtatttt gaattgaagt gagaatgagt aagttaagaa tagataagaa caaatgtttg
    17341 cagcgttcat ggacaggaaa aatggcgtat ggtatactaa acagtaactg gtcatggagg
    17401 tttaaacatg ggttctgata agatttatat tgctattgat ctgaaatctt tttatgcttc
    17461 cgtagaagca gtggatctcg ggctggatcc gcttactgct aatctggtgg tggcggatcc
    17521 ttcaaggacg gaaaagacca tctgtctggc ggtatcccct tctttgaagg cttacgggat
    17581 ctctgggcgg gcgagattat ttgaagtggt gcagaaggtg aagcaggcta atagtctgcg
    17641 aagaagcgga ttgccacagc gaaagtttac agggagatct tgtgatgcga aaatgcttga
    17701 aagggatccg cggcttgaga ttgattatgt aatcgcgccc cctcagatgg ctcactatat
    17761 ggaagtgagt acaaagatct atcatattta tttaaagtat gtggctccgg aggatatcca
    17821 tgtatattcc attgatgaag tatttatgga tgtgacagag tatctgggca cttacaggat
    17881 gacagcccgg gaactggcga tgaaaatgat ccaggatgtg ttaagtgaaa caaagatcac
    17941 tgccactgcg gggatcgccc ctaatctgta cctttgcaag gttgccatgg acattgtggc
    18001 aaaacatgtg gctgcggatc aggacggagt gcggatcgca gagctggatg aaatgtctta
    18061 tcgccagatg ctatgggatc acaggccgct tacggatttc tggagagtag gaaaaggtta
    18121 tgccagaaaa ctggaagaaa atggaattta tacaatgggg gatattgaca ggtgttctct
    18181 gggacgttct ggggattttt ataatgagga tctgttgttt cgactgtttg ggatcaatgc
    18241 ggaacttctc attgatcatg catggggata tgagccatgt accattgcag atataaaggc
    18301 gtacaagcct caggataaga gcattggctc ggggcaggta ttgcattgtc cctgtgattt
    18361 taaccaggca aagctggttt taaaggaaat ggcggatatg ctggcaatgg acctggtgga
    18421 taaagggctt atagccagcc agatcgttct gacggtgggg tatgatattg aaaatctgac
    18481 ggatccggtg agaagaaaag cgtataaagg ggcagtgact acggatcagt atggcaggaa
    18541 aatacccaag catgcccacg gaaccattca tttaaaactg ccgacttctt ctgcaagact
    18601 gatcatgcag ggggctatgg agctgtatga tggtattgta gataaaaatc tgctgatccg
    18661 caggctttat attacggcag aacatgctat ggaagagaag aaggtacaaa aacaggagac
    18721 ttatgagcag ttaagccttt ttacagacta tgaagcagtg gagaaggagc gagaaaagga
    18781 agtaaagaac ctggagaagg agaaaaggtt gcagcaggct atgttggata ttaagaagaa
    18841 gtatgggaaa aatgcgatcc ttaaggggat gaatttggag gaaggagcaa cggctataga
    18901 gcgcaaccgg cagatcggag ggcataaggc gtgaaaaagt atgaggatat cattggtctg
    18961 tcaagaccgg tttcaaaaaa gcatcctcct atgtcaaggg agaaccgggc ggcacagttt
    19021 gcgccttttg cggctcttac gggatttgaa ggggctatta aggagactgc cagggtgaca
    19081 ggggagaaga tcgaactgga tgagacgcag aaaacgtttt tggatgaaaa acttaaggta
    19141 ctgctgcgcg aaaaaactcc cgccctcttt acgtattttc aaaaagatga acaaaaagag
    19201 gggggagctt atataactgt ttcagggatc gtgaaaaaaa cggatgctta tacccataag
    19261 atcgtcttag aagatggtac cgggatctgc gtggaagata tcctggagat acggaaactt
    19321 ctctgatccg gcttatactt cagcttcctg gtgctggtac atcagttcaa tgtatgcaag
    19381 gatcagcggt gcaacgcctt tcgcctcatt tttaacgatc ggttcgcgca tataataatc
    19441 aaaggttcct tcccgcattt ctttatttcc aagtcctgcc acaagacaga tcccgcccag
    19501 ttgcagttct ccgttttctt cagaaaggta agtgcgacag atcccttcaa atgctttctt
    19561 tccataggca aaataggatc cgtccaggaa accgagacgt acactcttca tgatcgcata
    19621 tgcaaaaatt gcggaaccgg atgtttcaag atagttggga tgaattccac cccggtttac
    19681 gacctgatac cacattcctg tttcttcatc ctgataggga agcatggaat caataagttc
    19741 cttgtagatc cgattcattt ctttcttttc gctgccaaga gtttcttctg gcattacttc
    19801 cattgtatcg atcagagcca tggcatacca gcccaatgca cgcagccaga agttgtcaga
    19861 taatcctgtg actttatcac accagaaaga cgttctggag tcatcataag catggtaata
    19921 caggccgttt ctcagatcac gcatcagatt gtacacattg acgaactgct gatagcagtc
    19981 tttgcaatta gtactgttgt tgtaagtaac ttcatattgc atataaaatg gctgtgccat
    20041 atagagtcca tccagccaga tctggtttgg ataaatttct ttgtgccaga aattaccagt
    20101 tgaagttcgg ggctgggtct ttaactggct gtataccagg tcaatagcga ggcggtattt
    20161 ttcttttcca gtcagatcat acagatcaaa gagggttttt ccagtattaa cattgtcaag
    20221 gttgtgttct ttaggatcat aagaatggat ggaaccatct tcttctacaa aatagtcaat
    20281 aaaccggtct gcaaaatcaa gatattcttc tttctgtgta atatgataaa gttccaaaat
    20341 agcattgatc atacagccat ccatataatt ccaggaaggt tttgcacctt gacggatttt
    20401 ttcaatattc cagatcggac gggacggact gcttttttct aaaagctggt caatgtagcg
    20461 gtcaagaatt ttcatagcaa tatagatttc ctttctgaat tgtacaaagt aaaaaagtat
    20521 acagtaaaaa ctgaaaacgt ttgcaactac tttgatttta acataa
//
LOCUS       DBMDUA010000027        20378 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1622_length_20378_cov_3.874538, whole genome shotgun sequence.
ACCESSION   DBMDUA010000027 DBMDUA010000000
VERSION     DBMDUA010000027.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 20378)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20378)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20378
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1622_length_20378_cov_3.874538"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="ACZPCL_03720"
     CDS             <1..721
                     /locus_tag="ACZPCL_03720"
                     /inference="COORDINATES: protein motif:HMM:NF019824.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HIR1161630.1"
                     /translation="PKNQAMFEDLGFYTILKTDRVLFMENKKTGFTDFMEKVKAESPE
                     DALKEGKVIGAIVANCNPFTLGHRYLIETALKQCDYLHLFVLSDKRTFYSAAQRYEMV
                     KEGVKDLDRVILHHTSDYIISAATFPTYFMKEKTEAGKANCRLDLELFGKRIAPELHI
                     TKRFVGTEPNCAVTDCYNVTMKEVLPGLGIEVTEIQRKEQEGTAISASQVRQAVKEGK
                     TEEIRGLVPESTWKHIAGERS"
     gene            718..1386
                     /locus_tag="ACZPCL_03725"
     CDS             718..1386
                     /locus_tag="ACZPCL_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009532919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR1161631.1"
                     /translation="MTKEERTLVIIDRLKKEYPDAGCTLDYNQAWKLLVSVRLAAQCT
                     DARVNIVVEDLYKKFPDVNSLADAPVEEIEAIVKPCGLGHSKARDIKACMTRLKEDYN
                     GNVPDDFNALLKLPGVGRKSANLIMGDVFGKPAIVTDTHCIRLVNRMGLVDGIKEPKK
                     VEMALWKLVPPEEGSDFCHRLVYHGRAVCSARTKPDCEHCCVKDVCVTACGVGTFGTE
                     SAVR"
     gene            1745..2773
                     /locus_tag="ACZPCL_03730"
     CDS             1745..2773
                     /locus_tag="ACZPCL_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006907124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR1161632.1"
                     /translation="MKGIMKKAVFLAAAGMTALALSGCSAGSGGKRIVRISHAQSETH
                     PEHLGLLAFKEYVEERLGDKYEVQIFPNELLGSAQKAIELTQTGAIDFVVAGTANLET
                     FASVYEIFSMPYLFDSEEVYKSVMEDSDYMENVYESTDEAGFRVVTWYNAGTRNFYAK
                     TPINTPDDLKGKKIRVQQSPASVAMANAFGAAAAPMGFGEVYTAIQQGVIDGAENNEL
                     ALTNNKHGEVAKYYTYNKHQMVPDMLVANLKFLNSLSPEEYQVFKEAAQKSTEVELEE
                     WDKSIDEAKKIATDDMGVEFIDVDVNAFKQKVLPLHEKMLNENPKIRDLYEHIQEANE
                     AAAGKEGK"
     gene            2773..3276
                     /locus_tag="ACZPCL_03735"
     CDS             2773..3276
                     /locus_tag="ACZPCL_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR1161633.1"
                     /translation="MENMRKVRKTMMKVLGYLIIFLFMMMTAVGSYQIITRYFFNRPS
                     TVSEELLTYSFTWMSLLASAYVFGKRDHMRMGFMADKLTGNFRKYLEIIIDLLSFSFA
                     AVVMVYGGCSITKLTMIQTTASLRVPMGYIYVMVPVSGILIMIFAAMNAVEMFHHEFK
                     EAGEVRV"
     gene            3273..4580
                     /locus_tag="ACZPCL_03740"
     CDS             3273..4580
                     /locus_tag="ACZPCL_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR1161634.1"
                     /translation="MTIAGLCGLIIFVLLAVMLIAGVPIAVALAVSSICAILPVLNMG
                     ASVLTGAQRIFSGISVFSLLAIPFFILAGNLMNKGGIAIRLINFAKLFTGNIPGALAH
                     TNAVANMLFGAISGSGTAAASAMGSIIGPIEEEEGYDRDFSAAANIATAPTGLLIPPS
                     NVMITFSLVSGGTSVAALFMAGYIPGILWGLACMVVIYVFAKKKGYRSTKKYTASEKI
                     KVFFQAIPCLFMIIVVIGGIISGIFTATEGSVVAVVYSMVLAIFFYKSIKPSELPKIF
                     LDSAEMTGIIIFLIGVSSIMSWVMAFTGIPTAVSTAMLGISNNRYVILFIINILLLIV
                     GTFMDMTPACLIFTPIFLPICQALGMNTIHFGIMMIFNLCIGTITPPVGTTLFVGVKV
                     GKTRIEQVIQPLLYYFGAIFIVLMLVSYIPALSLWLPGLLGYV"
     gene            4965..5951
                     /locus_tag="ACZPCL_03745"
     CDS             4965..5951
                     /locus_tag="ACZPCL_03745"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HIR1161635.1"
                     /translation="MTIRIGILGYGNLGKGVECAIKHNPDMELKAVFTRRDPSTLSIL
                     TEGAKVYSVNDAPAMKDEIDVLIICGGSATDLPKQTPEMAKYFNVIDSFDTHARIPEH
                     FEAVDKAAKESGHIGLISVGWDPGMFSLNRLYANAILPGGKDYTFWGKGVSQGHSDAI
                     RRIAGVKDARQYTIPVESALEAVRSGSEPELTTRQKHTRECFVVAEEGADLKKIEEEI
                     VTMPNYFADYDTTVHFISEEELLRNHAGIPHGGFVIRTGKTGWNNENSHVIEYSLKLD
                     SNPEFTSSVLAAYARAAYRLRKEGQTGCKTVFDIAPAYLAAEDGAYLRKHML"
     gene            5962..7974
                     /locus_tag="ACZPCL_03750"
     CDS             5962..7974
                     /locus_tag="ACZPCL_03750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR1161636.1"
                     /translation="MTYVQRTICPYDCPTSCGLLAETDGKKILRVKGDKTHPANKGLI
                     CGKMQHYEESINSEKRILTPLRRTGKKGSGQFMPITWEEAGNEIAERFLNICREDGPQ
                     AILPAVYSGVMGQIQRKCGEAFFNRLGACSLVLTLCASAKGAGYSAVAGKTGCLDPRE
                     LENSDFFLIWGSNIMATRLQILPLLARKRKEGKQVILIEACGRDSASLCDKAVYIRPG
                     TDGALALAMMHVLWREHLEDEDFLRNQADGYEQFLPEIRKATPEWAQGITGIPAEVIE
                     ELAHVYGKAKAPAIVLGSGLSRYGNGGMTVRLITILSAFTGAWKRPGGGICGCDHNLG
                     DFVDTKRITRPDLRKEPARIVNINQLSSALNAEGKDKIRAFYVYAGNPVNSVCNQTGL
                     LKGVAREDLFTVVHERFMTDTALYADIILPATFSVEQTDCFKPYGYGTFAVANKIIDP
                     PGQCKSNWDTFCFLAGKLGFEEDYFKQTAEDALEDLLSHPGKDLARLSEEQWQFLREG
                     GTISADFADHCDFKTPSGKFMIVNKSLKDPVPHYIPCYDGTYPFHLISVPDSHTLNSI
                     FLERDSLVENRGPMSLLLHPQDGAELGIQDGDPVTAWNDLAEVEFKAVFTDQIARKTA
                     AASGVYSSSITGQRFQVNALNHERLSDIGEATTLNDNTINIKRSTF"
     gene            7971..9272
                     /locus_tag="ACZPCL_03755"
     CDS             7971..9272
                     /locus_tag="ACZPCL_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HIR1161637.1"
                     /translation="MKQYFVPFKELRSLTRGEKFAVLSLIPLYFIVIGLFLQPINEII
                     PGIIRIVREPDVLITDYFAVGGIGAALINAGLTTLLSIALLYFSGKEMDGHTITSSCL
                     MFGFSLFGKNIVNIWAIMAGVVLYAKYHGTSLKRYLYVGLYGTSLSPIMTQMMHIGKF
                     PAPVCLMLSLLLGLAIGFVLPPLCTHVHYAHQGYSLYNVGFGAGIIATVVVSLAKSFG
                     IKVESRLIWSVGNNTLFAVLLMSLFGCMIASAVAVRGKTILESYRRILKTSGIGGTDY
                     LKDEGGATTVFNMGVNGLFATLFVLAVKGDLNGPTICGIFTIVGFSSTGKHLRNIAPI
                     MFGVYLASFTKTWAINQPSPILALLFSTTLAPVAGRFGPVAGIIAGYLHSSVALNVGI
                     VYGGMNLYNNGFAGGIVAIFLVPVVQSISDRRARARGELSL"
     gene            9342..9926
                     /locus_tag="ACZPCL_03760"
     CDS             9342..9926
                     /locus_tag="ACZPCL_03760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1161638.1"
                     /translation="MDERLKKQLDFALEIDKEKNILRQTHLSGHGRNENDAEHAWHMA
                     IMAYLLREYSNEPVDITRVMLMCLIHDVVEIDAGDTYAYDEEGKKTQKAREEAAKERI
                     YSLLPEDQKEELAAIFDEFEESKTPESKFAHAMDNLQPLMLNNSNDGGDWREHGVSAK
                     QVYGRQSRTKEGSEKLYEVTDQIIKKHREKGNLK"
     gene            9923..10411
                     /locus_tag="ACZPCL_03765"
     CDS             9923..10411
                     /locus_tag="ACZPCL_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1161639.1"
                     /translation="MKLAVIYDSKTNNTAAVAGYMAEAMNSLEGVEAKTFLYSEVDTE
                     FVKKCKGVVFGCPTYAAGPTSDFYTWMEKNAGGLNLAGKLGGVFATEQYIHGGADLTM
                     NALLIHLLVYGMMIYSGGASCGKPVIHMGPVEVSPKKEDFKELFEIFGKRFATQALKI
                     NG"
     gene            10441..>10952
                     /locus_tag="ACZPCL_03770"
     CDS             10441..>10952
                     /locus_tag="ACZPCL_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HIR1161640.1"
                     /translation="MKTAGNNRKTTSNRVALYGLLIALAFVLSYVETLFPVYLGAPGV
                     KLGLANLVTVIALYGLGVKEAFAINVVRVLLSGFTFGNMSSILFGMAGAVLSLFLMAV
                     CKKLRLFDMTGISIIGGVAHNIGQFLVAAFVTKTFGVFSYLPVLLIAGTVAGALIGLL
                     GGIILKRISR"
     assembly_gap    10953..10962
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11285..12118)
                     /locus_tag="ACZPCL_03775"
     CDS             complement(11285..12118)
                     /locus_tag="ACZPCL_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HIR1161641.1"
                     /translation="MAVNYAEKFSQTIDEQLSKDSLTDKAVNKDYDFDGVNKVNVYSV
                     ETVPLNDYDINAKSNRYGTPVELGNDVQTLELTQDKSFTFSIDRKNATDTMMAMSAAK
                     ALARELKEVVTPTIDRYRIAKYTAEALAAHTKEETLTTETAYSAFLEGSMVLFDAKVP
                     TEGRIAFVTSAYYKAIKLDKNFVSSGDKGQEIAVTGAVGTIDKTTIIVAPADYFVKGT
                     NFIICHPMAMTSPVKLADYKIHENPQGINGWLVEGRIYYDAFVLNNKKAAIYLSKQAA
                     K"
     gene            complement(12134..12721)
                     /locus_tag="ACZPCL_03780"
     CDS             complement(12134..12721)
                     /locus_tag="ACZPCL_03780"
                     /inference="COORDINATES: protein motif:HMM:NF025623.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HIR1161642.1"
                     /translation="MGFIDQIMGAMGMEAAQTVTEEPTSNADPQRTEETKTDEVKEKA
                     EKIQDLDDGTDQGEGASSEEKTYSRDELNDILNEKRKEWEEERLANLSKDEQISQLKK
                     ELFKANMKDQINNRLTEEGIPVKAAELIQYTDEAGTLKSLETVISTIKELVSDGITER
                     LRGKTPEGLGKSASTGFWGKPENDFETAFVKAMKN"
     gene            complement(12727..12897)
                     /locus_tag="ACZPCL_03785"
     CDS             complement(12727..12897)
                     /locus_tag="ACZPCL_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161643.1"
                     /translation="MIQQYENELARIRGQIRSMEQTGEADTDRRKYRQLKQIRSELQS
                     SIRQMKQYTKKG"
     gene            complement(12917..13207)
                     /locus_tag="ACZPCL_03790"
     CDS             complement(12917..13207)
                     /locus_tag="ACZPCL_03790"
                     /inference="COORDINATES: protein motif:HMM:NF020345.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HIR1161644.1"
                     /translation="MGKTPEELLNELSEADTALLGEDCQALIRAIGLEGFKKLIQEFS
                     GVQLYIPKQEKVCRYLRDKKIRQEFDGSNHKTLAKKYGVTEAWCRKIIASEF"
     gene            complement(13233..13508)
                     /locus_tag="ACZPCL_03795"
     CDS             complement(13233..13508)
                     /locus_tag="ACZPCL_03795"
                     /inference="COORDINATES: protein motif:HMM:NF020345.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HIR1161645.1"
                     /translation="MGASIRESQIRLLTPDQKEMAECIGFEAYSKLAAYYGGSRVYVA
                     KLASLELAERDSRIIRDYKAGKTSTAELAARYGVSPRRIREIIKKQQ"
     gene            complement(13593..14168)
                     /locus_tag="ACZPCL_03800"
     CDS             complement(13593..14168)
                     /locus_tag="ACZPCL_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161646.1"
                     /translation="MKKNTETAKIPMINQVLKDAALGHLAECGLPQSLISGVDCSSYE
                     RMEETLLEVKNCFYSSVRKNIEDTYGPSCRAYTPDDEYKHLMEYKERDYWKMFDNMDC
                     HTESNKYIANLYCVIGDHVKLGEFGKENAKLFEKERLLEKILETGVDEELLEKLKAYW
                     ELQGEIRGEVEREGFICGFRTAYHLLNECRS"
     gene            complement(14438..15346)
                     /locus_tag="ACZPCL_03805"
     CDS             complement(14438..15346)
                     /locus_tag="ACZPCL_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024793.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1161647.1"
                     /translation="MEKKEFREEFSQFMKQTGKSQKQVSKETGLSTSVISQFLDGKYT
                     GDNGKVQDILNKFLSISKDRLNSVSGTTYYPDLYNTKEVLYACWYAHKYNDIALVAGD
                     AGAGKTTALEHYRDSNAGVTMVTVNTCTTSATSVLKLIADKIGQATDGRRSAVMQELI
                     SQLSGSNRLIIIDEADRLSLQALQAIRDLNDQAHVGIILSGNDKIYKQMLTGQKGREF
                     DQIRTRIIVRKRVENNYTVEEMQHIFPGVPESCLAFLIQMANLESLRTAIKLYEIALE
                     YARISKEAVTLKMLKDTQKQLFGRMY"
     gene            complement(15357..17150)
                     /locus_tag="ACZPCL_03810"
     CDS             complement(15357..17150)
                     /locus_tag="ACZPCL_03810"
                     /inference="COORDINATES: protein motif:HMM:NF020606.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1161648.1"
                     /translation="MTWLKVKEAARLLDLTDSAIKKAIKKNKYEFRHVEGIGRGGVQI
                     EIALESLPQEVQDKYNNIQREHTHNDMMQFTGKQREEANFKALIVENYQKAQKSPDDY
                     IKDFNERNPDSVITKSQLFRWQRKYKNGDVAGLIDTRGGHNRGKTDIPPDAWEYFYAL
                     YMTLQKRSIEICWEYTKTEYPEIPSVSAFERKVKTIPEPVIIFYREGEKAFNDSLVCM
                     ERSKEDIHSNDIWFSDHHLMDVAVLNNRGRVFRPWLTAFFDARSNKVIAFIIRDKSAD
                     ATVIKQCLRKGMEEFGVPKELYFDNGKDYREKSFNKDFPLSLTKQLGINQIYATPYHG
                     QAKPIERFWETLEDRFCKFFPTYLGKDAKKRPEDMRVTFEKLKDKCPTMEQFTDLLGK
                     HMNKYNNTPSGGIDMDGKCPDQVYFENLAVKNEVHDKSILRILCGTFDERTVQKNGVQ
                     YQGRFYYHTDLIAHQGEKVIINYDPSNMDELNVFDENMRAICKAAAKVRTPFRHTTQE
                     QIREAMKEKKNARKLVEQYAPTRDLDVMAIVARNQLVEKQFTEGEHTKTIENINPVLS
                     ENSKILEESTKQRRTSREENLLGKLMEASNQ"
     gene            complement(17163..17402)
                     /locus_tag="ACZPCL_03815"
     CDS             complement(17163..17402)
                     /locus_tag="ACZPCL_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1161649.1"
                     /translation="MPRPTNMKTKTRFGVLICNQLSKMGKNQKDLAQESGLSPFYVSE
                     ILNGKHNPSVRAVYNISKAIGISIEELTKTLLDES"
     gene            17532..18137
                     /locus_tag="ACZPCL_03820"
     CDS             17532..18137
                     /locus_tag="ACZPCL_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024252.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1161650.1"
                     /translation="MEHIGERIKIIRKMYNIKQKEFAERVSVSASYISKVESGKEVPT
                     DMLLKLIALDFKISLDWLKDGIGEIKESSKGFDTITDEGLNSKYQTMKDFLEKQIVEQ
                     TGDNLKNIVQAYSHFVSLVTMPGLNNDNQSRYLEALHGVIDILEQITYSSYMLKSVSD
                     NNYKALLQHKTKVDTGISKIDDDIKKALSCYLTQYGKDLTL"
     gene            18841..19680
                     /locus_tag="ACZPCL_03825"
     CDS             18841..19680
                     /locus_tag="ACZPCL_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02452 family protein"
                     /protein_id="HIR1161651.1"
                     /translation="MSSVENVEIFEDTKRLCETNGKIKDTLARSVKNQKLILERLELS
                     AVDKARFTDEAKIIVSTKRTFEAAAGYAGQKVAVHNFASATNPGGGVTRGSSAQEECL
                     CRCSDLYFCLSVPEMMKGFYYPHRNAKNPINNADIIYTPDVTVFKTDTNKPKLMDEKD
                     WYEVDVITCAAPNLRERPSNRFNQGNGDRAVKVSGRELLEIHKKRLTRILDVAVLNCD
                     EVVILGAFGCGAFQNKPEVVARAAKEVIADYMHAFKTIEFAVYCPPRDDTNFKVFKRV
                     MGV"
     gene            19702..20340
                     /locus_tag="ACZPCL_03830"
     CDS             19702..20340
                     /locus_tag="ACZPCL_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161652.1"
                     /translation="MGENYVYMDDDEMMELQDMLDDMLSEESFSMNALDGATVYADPS
                     PDYLLRVLCTPVLDRCFCLTMWGDRSYEHILHDIKDGTYQGLTMDVFEKKRLEWVKEI
                     KNTEDPILRVEKALRYAREVEVWETKKHFLDLVAKDKTTMVCAEVCSNMIKKGYSLTE
                     IAEIVPRTTKADIYGISLMIKRPLELTQKEYKAVEKEYRKTGEPAVLKKVFG"
BASE COUNT         5492 a         4275 c         4816 g         5785 t
ORIGIN      
        1 accgaaaaat caggctatgt ttgaggacct gggtttttat actattttaa agacagacag
       61 agtcttattt atggaaaata aaaagaccgg gtttacggat tttatggaaa aagtaaaggc
      121 agaaagtccg gaagatgcct taaaagaagg aaaagtcatt ggtgccattg tggcaaactg
      181 taatccattt acattgggac accgttatct gattgaaaca gcattaaagc agtgcgatta
      241 tcttcatttg ttcgtgcttt cagataagcg taccttttac agtgctgccc agcgctatga
      301 aatggtaaaa gaaggagtga aagatttgga ccgtgtgatc ctccatcata cgtctgatta
      361 tattatttcc gcagctacct tcccgactta ttttatgaag gaaaagacag aagcaggaaa
      421 ggcaaactgc cgtctggatc tggaattatt tggaaaaagg atcgcaccag aactgcatat
      481 tacaaagcgt tttgtaggaa ctgagccaaa ctgtgctgtt acggactgtt ataatgtgac
      541 tatgaaagag gtccttccgg gattgggaat tgaagtaaca gagatccaaa gaaaagaaca
      601 ggaaggaacc gccatcagcg cttcccaggt gcgtcaggca gtaaaggaag gcaaaacaga
      661 agagatccgg ggcctggtac cggaaagtac ctggaagcat atagcaggag agagatcatg
      721 acaaaagaag aacggaccct tgttatcata gaccgtctga aaaaagaata tcccgatgca
      781 ggctgcacct tagactacaa tcaggcctgg aaactgcttg tcagcgtccg tttagcagcc
      841 cagtgtacag acgccagagt caatatcgta gtggaagacc tgtataaaaa gtttccagat
      901 gtaaattccc tggcagatgc accggtggaa gagattgaag ctattgtaaa gccatgtggc
      961 ctgggacaca gcaaggcaag ggatatcaaa gcctgtatga cccggttaaa agaagattat
     1021 aacgggaatg ttcctgatga ttttaacgct cttttaaagc tgccgggagt aggcagaaaa
     1081 agcgccaatc tgatcatggg agatgtgttt ggaaaacctg ccattgtaac agatacccat
     1141 tgtatccgtc tggtaaaccg tatgggattg gtagatggca tcaaagagcc gaaaaaggtg
     1201 gaaatggccc tttggaagtt agttccgccg gaagaaggaa gcgacttctg ccatcgtctt
     1261 gtgtaccatg gaagagccgt gtgcagcgca aggacgaagc cggactgtga acattgctgc
     1321 gtaaaggacg tgtgtgttac tgcgtgtggg gtaggcactt ttggaaccga aagtgccgta
     1381 cgataaagta atggttgtga gaatttgccg tatttaaatg cggagccacg gcaaattctg
     1441 ttgctggtca tgcaacgcat gaacagtaac acgtgtgtga aaaagtgggc gtagaaggct
     1501 gaaaaatgcc ctgttaagaa gcaataaaaa atacgctaaa aagctgttaa tacaatttaa
     1561 taaaaagaca aaatagtctt gtagaaggca aaaattgtcc agagaaatcc ctctcttttt
     1621 ttattatact tagtgcataa gttccaagaa gattgatgcc gggaaagctg ctgttggcag
     1681 atatgacagg taaatgatct gagatgagaa caaaatccag tataaaaaag agagggatat
     1741 ttttatgaaa gggatcatga aaaaagcggt tttccttgca gctgccggta tgacggcgct
     1801 ggcgctttcc ggatgttcag cgggcagtgg cggaaagcgg atcgtgcgta tttcccatgc
     1861 gcagtctgaa acacatccgg agcacctggg gcttttagca tttaaggaat atgtggaaga
     1921 acgactggga gataaatacg aggtacagat ctttccaaat gaattgttag gatcagcaca
     1981 gaaagccatt gagctgacac agacaggtgc cattgatttc gttgtggcag gtactgccaa
     2041 tctggaaact tttgccagtg tatacgagat cttcagtatg ccgtatctgt ttgactccga
     2101 agaggtttac aagtccgtaa tggaagactc cgattacatg gaaaatgtat acgagtccac
     2161 agacgaggca ggtttccgtg tggttacctg gtacaatgca ggaaccagaa acttttatgc
     2221 gaaaacacct attaatacac cggatgatct gaaaggcaaa aagatccgtg tacagcagag
     2281 tcctgccagc gttgccatgg caaatgcttt tggtgcagca gcagctccca tgggctttgg
     2341 tgaggtttat acagccattc agcaaggcgt gatcgacgga gcagagaaca acgagctggc
     2401 gctgaccaac aataagcacg gcgaagttgc aaaatattat acatacaaca aacatcagat
     2461 ggtgccggat atgctggttg caaacctgaa attcttaaac agcttaagcc cggaagaata
     2521 tcaggtattt aaagaggcag cccaaaagtc cacagaagta gagctggaag agtgggataa
     2581 gagcattgat gaggcaaaaa agatcgcaac tgatgatatg ggagtggaat ttatcgatgt
     2641 ggatgtcaat gcatttaaac agaaggtact tcctctccac gaaaagatgt taaatgaaaa
     2701 tccaaagatc cgtgatcttt atgaacatat ccaggaagca aatgaggcag cggccggaaa
     2761 ggaaggcaag taatggaaaa tatgcgaaaa gtaagaaaaa ccatgatgaa agtgctgggc
     2821 tatctgatca ttttcctgtt tatgatgatg acagcagtgg gaagctacca gatcatcaca
     2881 agatatttct ttaaccggcc aagtacagta tcagaagagc tgctgaccta ttcctttacc
     2941 tggatgtccc ttctggcgtc tgcctatgtt ttcggcaaga gagaccatat gagaatggga
     3001 tttatggcag ataaactgac cggaaatttc agaaaatatc tggagatcat cattgatctg
     3061 ttatccttta gctttgcggc agttgttatg gtatacggcg gctgttctat tacaaaactg
     3121 actatgatcc agactacagc atctttgcgt gtgccaatgg ggtatatata cgtgatggtg
     3181 cctgtaagcg gtatccttat tatgattttt gcggctatga acgcagtgga aatgttccat
     3241 catgagttca aagaagccgg ggaggtgaga gtatgacaat cgcaggactt tgcggactga
     3301 ttatatttgt gttgctggcg gtcatgctga ttgcaggcgt acctattgct gtcgcattag
     3361 ctgtttcttc tatttgcgcg atcttaccgg ttttaaatat gggtgcaagc gtactgaccg
     3421 gtgcccagag aattttctcc ggtatctccg tattcagcct tcttgccatt cctttcttta
     3481 tcctggcagg aaacctgatg aataaaggcg gtatcgctat ccgtcttatc aattttgcaa
     3541 agctttttac aggcaatatc ccaggtgcat tagcccacac caatgcagtg gcaaatatgc
     3601 tgttcggtgc catttccggt tccggtacag cggcagcttc tgctatgggt tccattattg
     3661 gacctattga agaggaagaa ggttatgacc gtgatttctc tgcagcagcc aacattgcaa
     3721 ctgctcctac aggactttta attcctccaa gtaatgttat gatcacattt tccctggtca
     3781 gtggaggaac ctctgtagca gctcttttca tggcaggtta tatcccgggt atcctctggg
     3841 gccttgcttg tatggtagtt atctacgttt tcgcaaagaa aaaaggctat cgcagcacaa
     3901 agaaatatac tgcttctgaa aagatcaaag tatttttcca ggcgatccca tgcctgttta
     3961 tgatcattgt tgttatcggc ggcattatct ccggtatatt tactgctaca gaaggaagtg
     4021 tagttgcagt tgtatacagc atggtactgg caattttctt ctacaaatcc attaagcctt
     4081 ctgaactgcc gaagatcttc cttgacagtg cggaaatgac gggtatcatc attttcctga
     4141 tcggcgtatc cagtattatg agctgggtca tggcatttac aggtatccca actgcagtaa
     4201 gtactgctat gcttggtatc agcaataacc gttacgtgat cctgtttatt atcaatatcc
     4261 tgctgctgat cgtaggtact tttatggaca tgacaccggc gtgcctgatc tttaccccga
     4321 tctttttgcc gatctgtcag gcactgggaa tgaacaccat tcatttcggt atcatgatga
     4381 tctttaacct gtgtatcgga accattacac cgccagttgg aactacactg tttgtaggtg
     4441 taaaagtagg aaaaacaagg atcgagcagg tgatccagcc attgctttat tactttggag
     4501 ccatctttat cgtactgatg ttagtttcct acattccagc gctgtcactt tggctgccgg
     4561 gactgctggg atatgtataa aaaataggag atctgcctgc tgaggcaggt ggaaaggtgc
     4621 agcagaacag gcaaggcgca taagaccccg gaaattttat accccggggt ctttttttgt
     4681 tgccaacttt ttttgcatat agtataattt ctgatgatac caggggacaa agtttggaag
     4741 accgacgcaa gcttgcttgc aggaggactt gcaaaattgg gacccgccaa aaacatgagt
     4801 aagaagccat ttttgttaaa aaatgagcgg attacgaatg ttttctagga ttgcgggagc
     4861 acacagtgcg gagcaatccg gtatcatcaa aaagaccgaa ttacagggga cgcaaggcat
     4921 ttagcaatat gcagtggccc tggtgacaga aaggaagaac gaacatgaca atcaggatcg
     4981 gtattttagg ttatggtaat ctgggaaaag gagtagagtg tgccattaag cacaatccag
     5041 atatggaact gaaggcagta tttacaagaa gagatccttc caccctttcc attctcacag
     5101 aaggcgcaaa agtttattca gtgaatgacg cacctgcaat gaaagatgag atcgatgttc
     5161 tgatcatctg cggcggcagc gctacagatc ttccaaaaca gactccagaa atggcaaaat
     5221 atttcaatgt aattgacagc tttgatacac acgcaagaat tccggaacat tttgaggcag
     5281 tagataaggc agcaaaggaa agcggccata tcggactgat ctccgtaggc tgggatccag
     5341 gtatgttctc cttaaaccgt ctgtatgcaa atgctatcct tccaggcgga aaagattata
     5401 cattctgggg caagggcgta agccagggac attctgacgc catcagacgg atcgcaggcg
     5461 ttaaggatgc aagacagtat accattccgg tagaaagcgc tttagaggca gtaagaagcg
     5521 gcagtgagcc ggaactgact accagacaga aacataccag agagtgcttt gtagtggcag
     5581 aagaaggcgc agatcttaag aagatcgaag aagagatcgt taccatgcca aactactttg
     5641 ccgattatga tactactgta cactttatca gcgaagagga gctgttaaga aaccatgcag
     5701 gtatcccaca tggcggcttc gtgatccgta caggaaagac cggctggaac aatgaaaatt
     5761 ctcatgtaat tgaatacagc ttaaaacttg actccaaccc tgaatttact tcttctgtac
     5821 tggctgctta tgcaagagct gcataccgtt taagaaaaga aggacagact ggatgcaaga
     5881 ctgtatttga tattgcacca gcttatctgg cagcagaaga cggagcatat ttaagaaagc
     5941 acatgctgta ggagaaatac tatgacttat gttcagagaa ccatttgccc ttatgactgt
     6001 ccaacatcct gtggactgtt agcagaaacc gacggaaaga agatcttacg tgtcaaaggt
     6061 gataagaccc acccggccaa taaaggtctg atctgtggga agatgcagca ttatgaggag
     6121 tccataaaca gtgaaaaaag gatccttacg cctcttcgcc gcaccggcaa aaaaggcagc
     6181 gggcagttta tgcccattac ctgggaagaa gcaggaaatg agatcgcaga gcgttttctg
     6241 aacatatgca gggaggatgg cccccaggct atccttcccg ctgtctattc tggagtcatg
     6301 ggccagatcc agagaaagtg cggggaagca ttttttaacc gtttaggtgc ctgttccctg
     6361 gtactgactc tctgtgcatc tgctaaaggt gccggttaca gtgccgttgc cggaaaaacc
     6421 ggctgtctgg atccgagaga actggaaaac agcgatttct ttctcatctg gggaagcaat
     6481 atcatggcta ccaggctgca gatcttacct cttcttgcca gaaaacggaa agaaggaaaa
     6541 caggtgatcc tgatcgaagc ctgtggcaga gacagtgcct ctctctgcga caaagcggtc
     6601 tatatccgtc ctggaacaga cggggcttta gctcttgcca tgatgcatgt tctctggcgg
     6661 gagcatttag aagatgagga tttcttaagg aaccaggcgg atggctatga gcagttttta
     6721 ccggagatcc ggaaagctac gcctgaatgg gcacagggca ttacggggat ccctgctgag
     6781 gtgatcgaag aactggcaca tgtttacgga aaagcaaaag cgcctgctat tgtactggga
     6841 agcggtcttt cccgttacgg aaacggtgga atgaccgtgc ggctgatcac tatcctttct
     6901 gcttttacag gagcctggaa gcggccaggc ggtggcatct gcggatgcga ccacaatctg
     6961 ggtgattttg ttgatacaaa gcggatcaca agaccggatc tcagaaaaga accggcacgg
     7021 atcgtaaata tcaaccagct ttcatctgct ttaaatgcag aaggaaaaga taagatcagg
     7081 gctttttatg tatatgcagg aaatccggtg aattctgtat gcaaccagac ggggctttta
     7141 aagggagttg cgagggaaga cctgtttaca gtggttcatg agcggtttat gacagataca
     7201 gctctttatg cggacattat cttaccggcc actttttctg tggagcagac agactgcttt
     7261 aagccatacg gatatggtac ttttgcagtg gctaataaaa tcattgatcc gccgggacag
     7321 tgtaagagca actgggatac cttctgtttt ctggccggga agttgggatt tgaggaagat
     7381 tactttaaac agacagcaga agatgccctg gaggatctgc tttcccatcc cggaaaggat
     7441 ctggcgcggc tgtcagaaga acagtggcag tttttaagag aaggcggcac gatttctgca
     7501 gattttgcgg atcactgtga ttttaaaaca ccttccggga agtttatgat cgtaaataag
     7561 tcgttgaagg atcctgttcc ccactatata ccgtgttacg acggtacata tccattccat
     7621 ctgatctcag tgcctgacag tcatacctta aattctattt ttttggagcg tgacagtctg
     7681 gtggaaaaca gaggccccat gtccctgttg ctccacccac aggacggagc agaactgggg
     7741 atccaggatg gagatccggt tacagcctgg aatgacctgg cagaggtaga atttaaggct
     7801 gtgtttacag atcagatcgc cagaaaaaca gcagcagcat ccggcgtgta cagctcctct
     7861 attaccgggc agcgttttca ggtaaatgcc ttaaatcatg aacgcctgtc tgatataggt
     7921 gaagctacga cactgaatga taacaccatt aacataaaaa ggagtacctt ttgaaacagt
     7981 attttgtacc atttaaagaa ttacgttccc tgacaagagg agaaaaattt gcagtattgt
     8041 ccctgatccc gttatatttc attgtcattg gcctgtttct tcagccaatc aacgagatca
     8101 ttccgggaat tatacggatc gtgcgggagc cggacgtact gatcacagac tattttgcag
     8161 taggaggaat tggagctgcc ctgatcaatg cggggcttac aactctgctc agcattgccc
     8221 ttctctattt ttcgggaaag gagatggacg gtcataccat tacttccagc tgtctgatgt
     8281 tcggattttc tctttttgga aagaacattg tcaacatctg ggccattatg gcaggtgttg
     8341 ttttgtatgc gaaatatcat ggtacttctt taaaacgtta tctgtatgta ggtctgtacg
     8401 gcaccagtct ttcccctatt atgacacaga tgatgcatat cggaaaattt cctgcgccgg
     8461 tctgtcttat gctgtcactg cttttgggac tggctatagg ctttgtgctg ccgcctttgt
     8521 gtacccatgt gcactacgcc catcagggat attccctgta taacgtagga tttggcgcag
     8581 gtattattgc aacggtagta gtatctcttg ctaaatcttt cgggatcaaa gtagaatccc
     8641 gcctgatctg gtctgttgga aataataccc tgtttgctgt tttacttatg tctttatttg
     8701 gctgtatgat cgcatcagca gtggcagtgc ggggaaagac gatcctggag tcttatagac
     8761 gtatcctgaa aacatcaggg attggtggaa ccgattatct gaaagatgaa ggcggagcta
     8821 ctactgtatt taatatgggt gtcaatggcc tgtttgccac tctgttcgtt ctggcagtaa
     8881 aaggggatct gaacggtcct acgatctgtg gtatttttac gatcgttggt ttcagctcca
     8941 caggaaagca tttaagaaat atcgccccta tcatgtttgg ggtctatctt gcaagcttta
     9001 ccaaaacatg ggcgattaac cagccatccc ctatccttgc gctgttattt tccactacac
     9061 tggcgcctgt agcaggaagg tttggcccgg tggcaggcat tattgcaggt tatcttcatt
     9121 cttctgttgc tttaaacgta ggaattgtat acggaggcat gaatttgtat aacaatggtt
     9181 ttgccggcgg tattgttgca atctttttag tacctgtggt acagtcgatc agtgacagaa
     9241 gagcaagagc gagaggcgag ctgtccctgt agtgttctgc ctgctgatgg gaataaaaat
     9301 tccaaaaaac atgaaatatg ggaaagaagg aaattgacta tatggatgaa cgtttgaaaa
     9361 aacagctgga ttttgccctg gagatcgata aggaaaaaaa tattctccgc cagactcatt
     9421 tatccggtca cggaagaaat gaaaatgacg cagaacatgc gtggcatatg gcgatcatgg
     9481 cctatctttt acgggagtat tccaatgagc ctgttgatat taccagagtc atgcttatgt
     9541 gtctgatcca tgatgtagtg gagatcgacg ccggggatac ttatgcctat gatgaagaag
     9601 gcaagaagac ccagaaggca agggaagagg cggcaaaaga acggatctat tccctgcttc
     9661 ctgaggatca gaaagaggaa ctggcagcta tttttgacga atttgaggaa agtaagacac
     9721 cggagtcaaa atttgcccat gcaatggaca atcttcagcc attgatgctt aacaacagca
     9781 atgacggagg ggactggaga gaacacggtg tcagcgcaaa gcaggtttac ggccgccaga
     9841 gccgcacaaa agaaggttct gaaaaactgt atgaggttac agatcagatc ataaaaaaac
     9901 atagggagaa ggggaattta aaatgaaatt agcagttatc tatgactcaa aaacaaataa
     9961 tacagcggca gtagcaggct atatggcaga ggcaatgaac agccttgaag gggtagaggc
    10021 aaaaacattc ctgtattcag aagtagatac agagtttgta aaaaaatgta aaggcgttgt
    10081 gtttggctgt cctacttatg cagctggtcc tacatctgat ttttatacct ggatggagaa
    10141 aaatgcaggc ggtctgaatt tggccggaaa attaggcggt gtatttgcta cagaacagta
    10201 catccatggc ggtgctgacc tgactatgaa cgcgctgctc atccacctgt tagtatatgg
    10261 aatgatgatc tattccggcg gagcttcctg tggcaaacca gtgatccaca tgggaccggt
    10321 agaagtcagc ccaaagaaag aagatttcaa agaactgttt gagatctttg gcaaacgttt
    10381 tgcaacacag gcgttaaaga tcaacggata aacctattat caaaaaggaa actacgaaaa
    10441 atgaaaacag caggaaacaa cagaaaaacc acatcaaacc gagtggccct ttatggtctt
    10501 cttatagctc tggcctttgt attaagctat gttgaaacat tattccctgt ttatctggga
    10561 gcgccgggcg tgaagctggg gttagccaat ctggtaacag tcattgccct gtatggcctg
    10621 ggagtgaaag aagcctttgc cattaatgta gtgcgtgtat tgttatccgg ctttacattt
    10681 ggaaatatgt cgtcgatcct gtttggtatg gcaggagcag ttttaagtct gtttcttatg
    10741 gctgtatgca aaaagctccg cctttttgat atgaccggta tcagtatcat aggcggagtt
    10801 gcccacaata tcggccagtt tctggtggct gcatttgtga caaaaacatt tggcgtattt
    10861 tcctatcttc cggtactgct gatcgcagga accgtggctg gagcactgat cggactttta
    10921 ggcgggatca ttttaaaaag gatcagcagg atnnnnnnnn nnaaaaaaaa aaaaaaactg
    10981 tttataaatt attaaaataa aaaagattat ttttttaaac ttaaagagta tcctgaaggt
    11041 aatggacatg atacaggagg ttttagagaa tggattttat gatcttagtg tcgcgcttcg
    11101 cgcaaaacgt aactgagctt cgcgcaaaac gtaactgaac cgttacagag ccctgttttt
    11161 tatgcaaagt tttatacatt tttttgcatt tggcacgaaa aaagatttac tttttaaaag
    11221 atttaaaccg ccctcaaacg aatttgaaag gcggtttaaa tcgtgtttaa tccatattta
    11281 acggctattt tgctgcctgc tttgacaggt aaatagctgc ttttttatta ttcagcacaa
    11341 aagcatcata gtaaattctg ccctctacaa gccagccgtt aattccctgt ggattctcgt
    11401 ggatcttgta atccgcaagc tttaccgggg aagtcatagc cattggatgg cagattataa
    11461 agtttgtacc ttttacaaag taatctgccg gggctacaat gatcgtagtc ttatcaatag
    11521 ttcccacagc cccagtaacg gcaatttcct ggcctttatc cccggaggat acgaaattct
    11581 tgtctaactt gattgctttg tagtaggcgg acgttacaaa ggcgatacgg ccttcggtgg
    11641 gcactttagc atcaaacagc accatggagc cttccagaaa tgcactgtat gctgtttctg
    11701 tggtaagtgt ctcctctttg gtatgtgctg ctaacgcttc ggccgtgtat ttcgcgatcc
    11761 tgtagcggtc gatcgtagga gttaccacct cttttaattc ccgcgccagt gctttggctg
    11821 cgctcattgc catcatggtg tctgttgcgt ttttacggtc aatgctgaaa gtgaaagact
    11881 tgtcctgtgt cagttccagt gtctgcacat cattcccaag ctccaccggg gttccgtatc
    11941 ggttgctttt tgcattgata tcataatcat ttaaaggcac tgtttcaaca gaatagacat
    12001 tgaccttgtt tactccatca aaatcataat ctttgttgac cgctttatct gtaagagaat
    12061 ccttacttaa ctgttcgtcg attgtctgag aaaatttctc tgcgtagttt actgccatcg
    12121 ttttacctcc ctgttagttt ttcattgctt tgacaaatgc agtttcaaaa tcattttccg
    12181 gttttcccca gaacccggta gaagctgatt ttccaagccc ttccggggtc tttccacgaa
    12241 gtctttccgt gattccatcg gataccagtt ccttaatggt ggaaatgacc gtttccaggc
    12301 ttttaagtgt accggcttca tctgtatact ggatcagctc cgcagccttt acggggatgc
    12361 cttcctccgt caggcggtta ttgatctggt ctttcatatt tgccttgaaa agttcctttt
    12421 tcaactggct gatctgttca tccttcgaga gattggcgag gcgttcctct tcccattctt
    12481 ttctcttttc gttcagaatg tcgtttaact catcccggga ataagttttt tcctcgctgg
    12541 aagctccttc accctgatcg gttccatcat ccaaatcctg gattttttca gctttttctt
    12601 ttacctcatc tgtcttggtt tcctctgtgc gttgcggatc cgcgttgctc gtaggttcct
    12661 ctgttactgt ctgggctgct tccatgccca tggctcccat gatctgatca ataaatccca
    12721 tgctcatcat cctttctttg tatactgctt catttggcgg atactggact gtaattctga
    12781 acggatctgc tttaactgac ggtactttct ccggtctgtg tccgcttctc cggtttgttc
    12841 catgcttctg atctgtcccc ggatcctggc taattcgttt tcgtactgct gaatcattaa
    12901 aaacaggctc atggctctaa aactcgcttg caatgatctt gcggcaccag gcttctgtaa
    12961 caccatactt tttcgccagt gtcttgtggt tgcttccgtc aaactcctgg cggatcttct
    13021 tatccctgag atagcggcat actttttcct gtttggggat atacagctgt actccagaaa
    13081 attcctgtat cagctttttg aaaccttcca gcccgatggc tcggatcagt gcctggcagt
    13141 cttcacccaa aagggctgta tccgcttcac taagttcatt taataattcc tccggtgttt
    13201 ttcccattgt gtagttcctc cacatccttg ttttactgct gctttttgat gatttcccgg
    13261 atacgcctgg ggcttacgcc gtaccttgcc gcaagttctg ccgttgacgt tttcccggct
    13321 ttgtaatctc tgatgatccg gctgtcacgt tccgcaagct ccaatgatgc cagttttgca
    13381 acataaacac ggcttccgcc atagtaggcg gccagcttgc tgtatgcttc aaacccaatg
    13441 cattccgcca tttccttctg atccggtgtt aaaaggcgga tctgtgattc cctaatagaa
    13501 gcgcccatct gtccatccct ccattctgga taatataaca atggggaacc gctttgacag
    13561 acaaaacagt tccccttact tttcgcgccg ctttatgacc tgcattcatt taagaggtgg
    13621 taagcggtgc ggaatccaca aataaaccct tcacgttcaa cttcgccgcg gatctctccc
    13681 tgcagttccc agtaggcttt taatttttcc agcagctcct catccacgcc tgtttccagg
    13741 atcttctcta aaagccgctc cttttcaaag agcttcgcgt tctcttttcc aaattcaccc
    13801 agctttacat ggtctccgat gacgcagtaa aggtttgcaa tatacttgtt gctttctgtg
    13861 tggcagtcca tgttgtcgaa cattttccag taatcccgtt ctttatattc catcaaatgc
    13921 ttatactcgt catccggagt ataggcccgg caggatgggc cgtaagtgtc ttcgatattc
    13981 tttcttacgg aagaatagaa gcagttcttc acttccagca gcgtctcctc catccgctca
    14041 tagctggagc agtccacgcc agagatcaga ctctggggaa gcccgcattc tgccagatgt
    14101 ccaagcgcgg catctttcag cacctggtta atcatgggga tcttagcagt ttctgtattt
    14161 ttcttcatgt tcatatcctc ctttggcttt atatcccttt taaaaccgtt catatccctt
    14221 ttaaaaccgt ttaaagcatt cgtggtaagg aagtctttat atatcgctga aaacggattc
    14281 ctgcgcccat ctgcgtctca tggtttggga acggctgcaa aaaatgattg caacaccggg
    14341 aacggcctgc tatactatct gtgaacactt gtgttccggc ttatgccgga tggaatagtc
    14401 agtcttgcag ggctggctat ttcttttttg cccttcatca gtacatcctc ccaaagagct
    14461 gcttctgtgt atctttcagc atctttaatg tgacggcttc cttactgata cgggcatatt
    14521 ccagggcaat ctcataaagc ttgatcgctg tacgcagact ttcaagattt gccatctgga
    14581 tcagaaaggc aaggcagctt tccggcactc ctggaaaaat atgctgcatc tcctcaacgg
    14641 tatagttgtt ttctacacgc ttcctaacaa tgattctcgt ccggatctgg tcaaattccc
    14701 ggcctttctg gcctgtaagc atttgcttat agatcttatc attgccagaa agaatgatcc
    14761 ccacatgtgc ctggtcgttt aagtcccgga ttgcctgtaa agcctgtaaa gacaagcggt
    14821 cggcctcatc aatgatgatc agacggttgc ttccggaaag ctgagagata agttcctgca
    14881 ttaccgcgct gcgccttccg tctgttgcct gtccgatctt atccgcgatc agctttaata
    14941 cagacgttgc ggatgtggtg caggtattta ctgttaccat ggtcacgcct gcgttgctgt
    15001 ccctgtagtg ttccagcgcg gtggttttcc cggctccggc atcccctgca acaagggcaa
    15061 tatcgttata tttatgggca taccagcagg catataaaac ttcttttgtg ttgtaaagat
    15121 caggatagta cgttgttcct gaaacgctgt ttaaacggtc tttactgatg cttaaaaact
    15181 tgtttaaaat gtcctgcacc ttcccgttat ccccggtgta tttcccatcc aggaactggg
    15241 agatgacaga agtagaaagc ccggtctctt tgctgacctg tttctgtgac ttccctgtct
    15301 gcttcataaa ctgggaaaat tcttcccgaa attctttctt ttccatgtgt tcctccttac
    15361 tggttgcttg cttccataag ttttcctaaa aggttctcct cgcggctcgt gcgtctctgc
    15421 tttgttgatt cctccaggat tttgctgttt tctgaaagaa cagggttaat gttctcaata
    15481 gtctttgtat gttcgccttc tgtgaactgc ttttctacaa gctggttgcg ggcaacgatt
    15541 gccataacgt ccagatccct ggtcggtgca tactgttcga ccagctttct ggcattcttt
    15601 ttttctttca ttgcttcccg gatctgttcc tgggtagtgt gacggaacgg ggttcttacc
    15661 tttgcggctg ccttgcagat cgcacgcatg ttttcatcaa aaacatttaa ttcatccatg
    15721 ttggacgggt cataattgat gataactttc tctccctggt gggcgataag atccgtatgg
    15781 tagtaaaatc ttccctggta ctgtacgccg tttttctgta cggttctttc gtcaaaggtt
    15841 ccgcagagga tccgcaggat tgacttgtca tggacttcat tctttacggc caggttttca
    15901 aaatatacct gatccggaca ttttccgtcc atgtcgattc cgccagatgg tgtattattg
    15961 tacttgttca tgtgtttccc aagcagatct gtgaactgct ccatggtagg acatttatct
    16021 ttcagcttct caaaggttac ccgcatatcc tccggtcgtt tcttcgcgtc ttttcccaga
    16081 taggtgggga aaaacttaca gaaacggtct tccagggttt cccagaaccg ctcgatgggc
    16141 tttgcctgtc catggtatgg tgtcgcgtaa atctgattga ttcccaactg tttggtaagg
    16201 gaaagaggga aatctttatt gaatgatttc tcgcggtaat cttttccgtt atcgaaataa
    16261 agttctttag gaactccaaa ttcttccatt cctttccgga ggcactgctt tatcacggtt
    16321 gcgtctgcgg atttatcccg gataatgaaa gcaataacct tgttggaacg tgcatcaaag
    16381 aaagcagtga gccatgggcg gaacacccgg ccgcgattgt ttaaaactgc tacatccatt
    16441 aaatggtggt cagaaaacca gatgtcattg gagtggatgt cttccttgct tcgttccata
    16501 catacaaggg aatcattgaa cgctttttcg ccttccctgt aaaagatgat cacgggttcc
    16561 ggaattgttt tgactttccg ttcaaaggca gatacggaag gaatctccgg atattccgtc
    16621 tttgtgtatt cccaacagat ctctatactg cgtttttgca aagtcatata cagcgcgtag
    16681 aaatattccc atgcgtccgg tgggatgtcc gtctttccac ggttatgccc tccgcgtgtg
    16741 tcgatcagcc cggcaacatc gccgttctta tacttgcgct gccagcggaa aagctgggac
    16801 tttgtgatca cactgtccgg gtttcgctca ttaaaatctt ttatgtagtc atccggggat
    16861 ttttgcgctt tctgatagtt ttctacgatc aatgccttaa agttggcttc ctcccgctgt
    16921 ttcccggtga actgcatcat gtcattatga gtatgttccc tttgaatgtt gttgtatttg
    16981 tcctgtactt cctgcggcaa cgactccagg gcgatttcga tttgaacacc gccccggcca
    17041 attccttcga catggcggaa ttcatattta tttttcttaa tcgctttttt aatagcacta
    17101 tctgttaaat ctaaaagcct agctgcttct tttactttca gccatgtcaa agttctcacc
    17161 gcctagcttt catcaagtaa ggttttagta agttcttcaa tgctgattcc aatcgctttc
    17221 gaaatgttgt acactgccct tacagatggg ttatgtttac cattaagaat ctcgctcaca
    17281 tagaacggtg agagaccaga ttcttgagca agatctttct gatttttccc cattttgcta
    17341 agctggttac atataagcac tccgaagcgt gtttttgttt tcatattagt aggtctcggc
    17401 atttctccac ctccttaacc gttgaaattt tttttcttct ttgttatatt tattgtgact
    17461 atctgtatta tagttgagta tactcaactt gtcaatacaa aagttgatta tactcatttt
    17521 aggaggttgc catggaacat ataggagaac gaataaagat aataagaaaa atgtataata
    17581 tcaagcagaa agaatttgcg gagcgcgttt ccgtatcagc atcatatatt agcaaagtcg
    17641 aatctggcaa agaggttcct acagatatgc ttttaaaatt gattgctttg gattttaaga
    17701 tttctcttga ttggttaaag gatggcatcg gagaaataaa ggagagtagc aaaggatttg
    17761 atactataac agatgaagga ttaaattcta aatatcagac catgaaagac tttcttgaaa
    17821 agcaaatcgt agagcagact ggagacaatc taaaaaatat tgttcaagcc tattcgcatt
    17881 ttgtcagcct tgtcactatg cctggcttga ataacgataa ccaatcgaga tatttggaag
    17941 ctcttcatgg tgtaattgat atactggagc aaatcacata tagttcgtat atgttgaagt
    18001 ccgtatccga taacaattat aaagctcttt tacaacataa gacaaaggtt gatactggga
    18061 tcagcaaaat agatgatgat attaaaaaag ctttatcctg ctatctcaca cagtacggaa
    18121 aagacttaac tttataggca tattttaaaa tagcatattt atacatattt tatgaatatt
    18181 tatacgtaac aaagttcctt tctttccctt agtccatttt taagaaatgg catccaaaaa
    18241 gtccgaaaaa tactgattta aacgtatttt tcggactttg tttaaattgc tatgaattcc
    18301 cttagtcagt tatttttgaa tggttagttg cgttttgttc ccttagtcaa caattacgtt
    18361 gttttaaatg cttttaagga gaaagccgca taaaataagg atttttgggg atttttaaag
    18421 tcttttaagg ttttttaagg gttcatgaat cccccaaaga ctaagggaat agttacattt
    18481 tactcacagg acaacaataa atccgacagc cgctatgaga agtatatgat ttactttttc
    18541 acaagtccgg caagtaaata ccccggcaaa tacctgcata caacctacgg aagcattcag
    18601 gtggatgatg gagcattgac aatcagaaca aagaacagtg tctatgaatt tgagctggat
    18661 gcatcatgcg tttcgaaagc ggacatgatt ttattgctgc atgcagtaaa tgagtatttc
    18721 cgtgatgatg gaatgtaagg cagacgatgg ttgccaggcg gctgcgggag caatttcgca
    18781 gctgttttta cagttccaga cagtatcgga atgaacggaa gaggaaggaa tgggaaacat
    18841 atgagcagtg ttgagaatgt ggaaattttt gaagatacca aaaggctgtg cgagacaaac
    18901 ggaaagatta aggacaccct tgcgcggtca gtgaaaaatc agaagctgat tctggagaga
    18961 ttggaactgt ctgctgtgga taaggcgcgt ttcacagacg aagcaaagat tatcgtatcc
    19021 acaaagcgga ctttcgaggc ggctgcaggt tatgcaggtc agaaggtggc ggtacataac
    19081 tttgcctctg caaccaatcc gggcggcggc gtgacaaggg gatccagcgc acaggaggaa
    19141 tgtctgtgtc ggtgttccga cctgtatttc tgcctgagcg taccggaaat gatgaagggg
    19201 ttctattatc ctcatcggaa tgcaaagaat ccgatcaaca atgcggatat catctatacg
    19261 ccggatgtga ccgtatttaa gacagatacg aacaagccga agctgatgga tgaaaaagac
    19321 tggtatgaag tggatgtgat cacctgtgcg gcaccgaacc tgcgggaacg gccgagcaac
    19381 cggttcaacc agggtaacgg tgaccgggcg gtaaaagtca gtggccggga actcctggaa
    19441 atccataaga agaggctgac caggattctg gatgttgcag tactgaactg tgacgaagtg
    19501 gtaatcctgg gtgctttcgg atgcggagcg ttccagaata agccggaagt ggtggcaaga
    19561 gctgccaaag aagtgattgc ggattatatg cacgcattta aaaccattga atttgcggtc
    19621 tattgcccgc cgcgggatga tacgaacttc aaagtgttta agcgggtgat gggagtgtaa
    19681 agcatcgaaa ggagacaagc gatgggagaa aactatgtat atatggatga cgatgagatg
    19741 atggaattgc aggatatgct tgatgatatg ctttccgagg agagtttttc aatgaatgcc
    19801 cttgatggcg caaccgtgta tgcggatccg tccccggatt atctgctccg ggtattgtgt
    19861 acaccggttc tggatcgctg cttttgtctg accatgtggg gtgacagatc atatgaacac
    19921 atactgcatg atatcaaaga cggcacgtat cagggactta ccatggatgt ttttgagaag
    19981 aaacgcttgg aatgggtgaa ggaaatcaag aatacggaag accccattct ccgtgtcgag
    20041 aaagcattac ggtatgcccg tgaagttgag gtctgggaga caaagaaaca ctttctggat
    20101 ctggttgcga aagacaagac cacgatggta tgcgcggaag tgtgctccaa tatgatcaag
    20161 aaaggctatt ccctgacgga aattgcggag attgttcctc ggaccaccaa agcggatatc
    20221 tacgggattt cccttatgat aaaaagaccg ctggaactga cgcagaaaga atataaagcg
    20281 gttgaaaaag aatacagaaa gacgggagaa cctgccgtat taaagaaggt gttcggataa
    20341 ccattggaaa ggatatagag gttatatttc cccgccaa
//
LOCUS       DBMDUA010000028        20369 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1623_length_20369_cov_2.963736, whole genome shotgun sequence.
ACCESSION   DBMDUA010000028 DBMDUA010000000
VERSION     DBMDUA010000028.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 20369)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20369)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20369
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1623_length_20369_cov_2.963736"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            182..802
                     /locus_tag="ACZPCL_03835"
     CDS             182..802
                     /locus_tag="ACZPCL_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4317 family protein"
                     /protein_id="HIR1161653.1"
                     /translation="MINRDDMLELTRRMTPARASVGRIAGAYFDEEGYVDGTFNTNFL
                     KLSASERTRNLNMAKTVLISKTNEQLKEYRITREMGKPGSVWQLLDGVKESEMKNDAY
                     MDLFYEVLGEKYHPGYPFSCFMYYGQYDVPVKGTDKEWMEGSEEVYSYLLCVISPLTG
                     EYEPGAPEFGFLYPAFKDRSTGWEFVNVYEKLPERVHREWMEWLFK"
     gene            862..1659
                     /locus_tag="ACZPCL_03840"
     CDS             862..1659
                     /locus_tag="ACZPCL_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033735 - aspartate dehydrogenase
                     [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase domain-containing
                     protein"
                     /protein_id="HIR1161654.1"
                     /translation="MTKVTTKKKLAVLGNGFLAGIIVGAYNKGLLEEYELTGILGRTK
                     EKTEALAEKGGCRPCSTLEELLEDKPDYVAEAASVKAVQDYGMKILSGGADMILLSIG
                     ALADETFYREISECAKENGRKIHIASGAIGGFDVLRTISLMGQAQTSFRTKKGPKSLS
                     GTPLFEESLMTDKEEKQVFHGSAKEAISILPTKVNVAIAASLASSGPENVDMSIFSVP
                     EMVGDDHKITAQIPGVKAELDIYSSTSAIAGWSVVAVLRNLVSPIVF"
     gene            complement(1733..2743)
                     /locus_tag="ACZPCL_03845"
     CDS             complement(1733..2743)
                     /locus_tag="ACZPCL_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HIR1161655.1"
                     /translation="MALFESYERRIDKINSVLNSYGIASIEEAEKITKDAGLDVYAQV
                     KKIQPICFENACWAYTVGAAIAIKKGCKRAADAAAAIGEGLQAFCIPGSVADQRKVGL
                     GHGNLGKMLLEEDTDCFCFLAGHESFAAAEGAIGIAEKANKVRQKPLRVILNGLGKDA
                     AQIISRINGFTYVETEMDYYTGEVKELWRKSYSDGLRAKVNCYGANDVTEGVAIMWKE
                     GVDVSITGNSTNPTRFQHPVAGTYKKECIEKGKKYFSVASGGGTGRTLHPDNMAAGPA
                     SYGMTDTMGRMHSDAQFAGSSSVPAHVEMMGLIGMGNNPMVGATVAVAVSIEEAAKAG
                     KF"
     gene            complement(2771..3463)
                     /locus_tag="ACZPCL_03850"
     CDS             complement(2771..3463)
                     /locus_tag="ACZPCL_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR1161656.1"
                     /translation="MIYSQEVEEMCKVAQGVHHGAAPIPEEAKWVKSKEIKDISGLTH
                     GVGWCAPQQGACKLTLNVKEGIIQEALVETIGCSGMTHSAAMAAEILPGLTVLEALNT
                     DLVCDAINTAMRELFLQIVYGRTQSAFSEDGLPVGAGLEDLGKGLRSQVGTMYGTLKK
                     GPRYLEMAEGYVTGIALDADDQIIGYQFVNLGKMTDFIKKGDDPTTAWEKAKGQYGRV
                     ADAVKIVDPRKE"
     gene            4228..4737
                     /locus_tag="ACZPCL_03855"
     CDS             4228..4737
                     /locus_tag="ACZPCL_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HIR1161657.1"
                     /translation="MALLETWRNLAYGDGLDDKKREELWSGYFQIEKGIYEQILSQPE
                     QVITGTVKELAEKYNTEILIMTGFLDGINESLKGYENPIDTMEEDTEVKIEIDPEKLY
                     YNMVEAKATWLYELPQWDAILTPEKRKELYKNQKASGTVRKAKKIFPNDPCPCGSGKK
                     YKKCCGKNA"
     gene            4846..5595
                     /locus_tag="ACZPCL_03860"
     CDS             4846..5595
                     /locus_tag="ACZPCL_03860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIR1161658.1"
                     /translation="MEAYTSFAQVYDLFQDNIPYGEWADYLKSLLNEYGVKDGLVLDL
                     GCGTGSITELLAKAGYDMIGVDNSEDMLEIAMDKRSRSGLDILYLLQDMREFELYGTV
                     KAAVSICDSMNYILEKEDLVQVFKLVNNYLDPSGIFIFDMNTEYKYTHLLADGTFAEN
                     REESSFIWENFYDEEERINEYDLTLFIKEGELFRKFEETHYQRCYSLDEVKEAAKEAG
                     MDFVAAYDAFTRESVKDDSERIYLVFRERGK"
     gene            5690..6571
                     /gene="hslO"
                     /locus_tag="ACZPCL_03865"
     CDS             5690..6571
                     /gene="hslO"
                     /locus_tag="ACZPCL_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR1161659.1"
                     /translation="MADYVIRATAAEGQIRAFAATTRDLTETARQAHNTSPVATAALG
                     RLMTAAVMMGYDMKGADDLLTIKIQGDGPIGGLVVTADSHGEVKGYAFNPGVMLPPND
                     KGKLDVGGALGVGVLSVIKDIGLKEPYVGQTILVSGEIAEDLTYYYATSEQTPSSVAL
                     GVLMNKDNTVRQAGGFIIQLMPGASDAVISALEEKIKEIHSITTLLDVGNTPETILQY
                     ILGDLGLEINEKLPAKFTCNCNKSRIEKALISVGKKDIQEMIDDGKPIEVNCHFCNKN
                     YTFDVEELKEILEKARR"
     gene            complement(6698..6895)
                     /locus_tag="ACZPCL_03870"
     CDS             complement(6698..6895)
                     /locus_tag="ACZPCL_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HIR1161660.1"
                     /translation="MKGTVKWFNNQKGYGFISDEQGNDVFVHYSGLNMEGFKSLDEGA
                     EVEFDVVNGAKGPQATNVTKL"
     gene            7135..7680
                     /locus_tag="ACZPCL_03875"
     CDS             7135..7680
                     /locus_tag="ACZPCL_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5662 family protein"
                     /protein_id="HIR1161661.1"
                     /translation="MKLVNIVKHFCTITRHRLLVCKHCFKIGLYWQGLTHDLSKYSPE
                     EFWTGVRYYQGDRSPNAAEREAIGFSKAWLHHKGRNKHHFEYWIDVSLNKEEGLVGNK
                     MPVNYVAEMLCDRIAACEVYRGKNYTSAAPMEYYEFTKKYITIHPETRALLEKLLTIL
                     KDGGEESAYAYIRKLLKKGSY"
     gene            complement(7737..8165)
                     /locus_tag="ACZPCL_03880"
     CDS             complement(7737..8165)
                     /locus_tag="ACZPCL_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HIR1161662.1"
                     /translation="MSEITKESRADRAIACHNKGYNCAQSVAFAFLDKVDIDPETLFK
                     VTEGLGLGMGGMEGTCGAISAAAVLAGLKISTADLEKPNSKGTSYKASKACIAAFKEK
                     NSTIVCRELKGVDTGKVLRACPDCIRDAVAIIEENLFPEE"
     gene            complement(8187..8969)
                     /locus_tag="ACZPCL_03885"
     CDS             complement(8187..8969)
                     /locus_tag="ACZPCL_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM164 family acyltransferase"
                     /protein_id="HIR1161663.1"
                     /translation="MAEAFFQATAWTMDTPKPYGAFHLVLTVLGVSLSIFLARKFSFP
                     ERKAGPDTILFLCGLFLGLTELYKQGFIYYIENGRTYDWWYFPFQLCSVPMYLGLFIP
                     MVPARIRNVFYLFIQDFGLLGGIMALAEPSGLMHPYWTMTVHGFLWHFNLIFMGLICA
                     FYNLKTETPKSYLHTIPLFLFCTAIATAVNVLSHPYGNADMFYISPYYPNGQIVFHQI
                     SLVIGTFAGNCLYLASVLLGGFIIHISCLEIGQHYFTKISKF"
     gene            9128..9556
                     /locus_tag="ACZPCL_03890"
     CDS             9128..9556
                     /locus_tag="ACZPCL_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1161664.1"
                     /translation="MAGIVTALLSGALMSVQGVFNTGVTKQTSVWVASGWVQATALIA
                     CVVIWFFTGRPPVRGLFSVSPIYLLSGGIIGAVITYTVIRSMQSLGPAQTALLIVVAQ
                     IITAYVIQLFGWFGTEKMPFEWHKLLGAAVAVAGIIIFQR"
     gene            9670..10365
                     /locus_tag="ACZPCL_03895"
     CDS             9670..10365
                     /locus_tag="ACZPCL_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1161665.1"
                     /translation="MKRTLSLKKKKQIAAALAAAVFVCGGCTGNLKEEQDEILLETVA
                     ESSENLEHADDAEQSEAGETLPETAEPQETAETRLGSTIYVHVCGHVKYPGVYQMQEG
                     SRVYEAIDKAGGMLEEGAGDAMNMAAVLEDGMRIMVPDLAQAAQMIEQGAVFDPGSSA
                     SVSDRKSSGKVNLNRADVKELMTLTGIGQSRAEAIIRYREEIGSFERIEDVMKVSGIK
                     ESAFNKIKDDITV"
     gene            10444..11148
                     /locus_tag="ACZPCL_03900"
     CDS             10444..11148
                     /locus_tag="ACZPCL_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1161666.1"
                     /translation="MARVLVVDDEKLIVKGIRFSLEQDGYEVDCAYDGEEAIEMAKKT
                     EYDIVLLDVMLPKHDGFEVCQAIREFSDMPVIMLTAKGSDMDKILGLEYGADDYISKP
                     FNILEVKARIRAIIRRSMKAKRQKKEEQANTYIGSGDLKMDTEGRRVFIGEKEINLTA
                     KEFDLLELLVRNPNKVYSREALLNYVWGNKAMDSGDVRTVDVHVRRLREKIEPSPSDP
                     KFVHTKWGVGYYFQAK"
     gene            11175..11654
                     /gene="rlmH"
                     /locus_tag="ACZPCL_03905"
     CDS             11175..11654
                     /gene="rlmH"
                     /locus_tag="ACZPCL_03905"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR1161667.1"
                     /translation="MKITLVTVGKIKERFFEDAIAEYSKRLGRYCKLEIIQVADEKTP
                     DGASENVERQIREKEGQRILAQIKDGAYVLALAIEGKMLSSEELAGKIQKLGVEGKGH
                     LVFVIGGSLGLSKEVMDRADYALSFSKMTFPHQLMRVILLEQIYRGFKIIAGEPYHK"
     gene            11859..12881
                     /gene="spoIIIAA"
                     /locus_tag="ACZPCL_03910"
     CDS             11859..12881
                     /gene="spoIIIAA"
                     /locus_tag="ACZPCL_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HIR1161668.1"
                     /translation="MAAKTEKGLKQEIVNLFPVRLRQILEALPLDFARLEEIRLRCGQ
                     PILFRIAGKEMGITGSGDLTELGSSGKLENWEKLEKIREKELKDTLEIMGGFSLYAAE
                     EELRQGFFTVRGGHRIGVAGRVILKDRQITGLKAASFLNVRVAHEIKGCADQVLPFLY
                     VNGKFVSTLIVSPPGCGKTTLLRDLIRQVSDGSSQKAGKCSFAGVNVGVADERSELGA
                     CYQGVPQNDLGMRTDVLDGCPKSEGMLMLIRSMAPAVVAVDEIGGKEDIKAVEYVRNC
                     GCALAATAHGNSLEELKERAGFRELLEEKTFERLVFLSRKRECRGKVEVVYDSGFNRL
                     YSGRTF"
     gene            12899..13429
                     /locus_tag="ACZPCL_03915"
     CDS             12899..13429
                     /locus_tag="ACZPCL_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HIR1161669.1"
                     /translation="MFLKWTGGLLILFAGSGMGVWLAWGYKKRLSTLENLRRMIYCLK
                     GEIVYSHAPLEEAFERIGKREKGVLGELFTQTAEKIGCHNGETFSKIWEQTIEEMEKN
                     GKPLFLEKEDREKLLSLGGQLGYLDTQMQERTLLLYLEQLELSVSRLRGEIREKCRLS
                     TVLGVMGSLFLVIVML"
     gene            13445..13639
                     /gene="spoIIIAC"
                     /locus_tag="ACZPCL_03920"
     CDS             13445..13639
                     /gene="spoIIIAC"
                     /locus_tag="ACZPCL_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HIR1161670.1"
                     /translation="MDVNLIFRIAAVGILVSVICQVLKHSGRDEQAFLTSLAGLVMVL
                     FWMIPYIYDLFETMKQLFLL"
     gene            13653..14039
                     /locus_tag="ACZPCL_03925"
     CDS             13653..14039
                     /locus_tag="ACZPCL_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAC/SpoIIIAD family protein"
                     /protein_id="HIR1161671.1"
                     /translation="MNMLTLGAAGIAAVLLAVWMKGVKAEYGIFVVIAAQLFFLFYGT
                     GRLGEILDTVRQLEQYIKIRPVYLITLLKMTGITYSAEFASGICKDAGYASLGKQIEI
                     FGKLTILAVSMPVLLALFGTLDQILT"
     gene            14052..15233
                     /locus_tag="ACZPCL_03930"
     CDS             14052..15233
                     /locus_tag="ACZPCL_03930"
                     /inference="COORDINATES: protein motif:HMM:NF021088.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HIR1161672.1"
                     /translation="MRKQANRWLVAVFLLFFIFSGIHMTAFGEEPAKDPLAQLEEEMG
                     IGEEIQRIQEYLDGLRPEGRTVSLTDLMQVIIQGNMKEIVNTAGKLLEDALFSQAKEG
                     QALLVQVAVLGLTGAVFSHAASAFKGSQIPETAFYMIYLLIFTCLAGSFLSSIQITAQ
                     VLDQILEFVRLLMPTYFISVAFAGGSTSAAAFYEIALGAAWAVQWLCRQVFLSIVRVY
                     GLLVLGDHMMEEPFFSKMTELMEKIVSWGLRSILGLTMGLQILQSLVLPYADSAGRSA
                     IMKFAEMIPGLGQATGAAARLVFGSSVLIKNSLGAAAVLILAVITLVPVVKLAVLMVM
                     YQGAAALLQPVCDKRIISCIQGMAAGHGLLLRITLYSLFLFILVIAITCAGTNVTYLA
                     A"
     gene            15290..16015
                     /locus_tag="ACZPCL_03935"
     CDS             15290..16015
                     /locus_tag="ACZPCL_03935"
                     /inference="COORDINATES: protein motif:HMM:NF021121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HIR1161673.1"
                     /translation="MGEILLEWARNIIFFMVFLSVISHLLADASYEKYIRFFAGIVLI
                     LITVSPLKGGLDFQEKAGRFFEEFSFFQEKEQAGKALSKADQERMGAFLAEYKKEAEA
                     RIRETMQAEGVICSQAEVEIEKDEKSGEYGKIRKIILHLKEEKMTSPQDEETKMVKEK
                     AGEEVSVKVEIPPVITKEKQERPGTESKQEQQLKRKVAQYYGLEEACIEIWWEDDQGE
                     MAVSASVRSDPDDPVYAISGKQQ"
     gene            15930..16547
                     /locus_tag="ACZPCL_03940"
     CDS             15930..16547
                     /locus_tag="ACZPCL_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007036943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HIR1161674.1"
                     /translation="MTREKWLFLLLLGVILMILSMPFPGSSSKKTGQTKGENVSLWEK
                     NSSGDGQQTEENIVQEPVQAASAKPAQDYEKELEDRISQLLKSVEGVGKVDVMVVLSS
                     SGEKVFRTDRSENTSTTKEKDASGGERDAVSSQLEENTVFSQSAGQNTGNGPLIEKEL
                     YPQISGIVISAQGGDLPSVQAEISQAMEALFGLEPHKIKVLKRVE"
     gene            16563..17366
                     /locus_tag="ACZPCL_03945"
     CDS             16563..17366
                     /locus_tag="ACZPCL_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HIR1161675.1"
                     /translation="MKIRNMKQVGERVKEIKMKRLFRRNQIIITTLAVMIAAAGYLNY
                     AGKRDLEAGGQVADMGSLELSDEDLMAENLAAQSETVQDILSLDNDPNDTEVAEAQTE
                     GTDLAQAGSENGEQSGMADTEEQTTGNDAPAGYENPGEAVLTSGMSVADYIAGVQLSR
                     EQIRAKNKETLMSLINSTSIDEAAKQQAIQDMIRLTEISEKENAAETLLMAKGFSDPV
                     VSITDDKVDVVIHASSITDPERAQIEDIVKRKAEVGADQIIITLLNMAD"
     gene            17449..17829
                     /locus_tag="ACZPCL_03950"
     CDS             17449..17829
                     /locus_tag="ACZPCL_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR1161676.1"
                     /translation="MAEESRSTHKLYEKDKIGEVQIADEVVAIIAGLAATEVEGVDSM
                     AGNITNELVGKLGMKNLSKGVKVDVTEEHVSVDLSLNIRYGYNIPAVSEQVQEKVSSA
                     IENMTGLTVLDVNIKIAGVNMEES"
     gene            17976..18467
                     /gene="nusB"
                     /locus_tag="ACZPCL_03955"
     CDS             17976..18467
                     /gene="nusB"
                     /locus_tag="ACZPCL_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR1161677.1"
                     /translation="MTRRELREHCFKMLFSVDFYTTGEEAKAQMQQYFQSPEEDDTAE
                     DGSLQVVHKVAAGEKDREYLSTRTADVVDKIEEIDAKIDEVAAGWKTKRMGKVELTIL
                     RLAVYEMLYDDTIPEKVSVNEAVELAKKFGGNESPAFVNGVLAKFIPKEEKKEESKED
                     QEA"
     gene            18468..19751
                     /gene="xseA"
                     /locus_tag="ACZPCL_03960"
     CDS             18468..19751
                     /gene="xseA"
                     /locus_tag="ACZPCL_03960"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR1161678.1"
                     /translation="MGSVYTVSQVTSYIKNMFTQDFALNRVSIKGEVSNCKYHTSGHI
                     YFTLKDGGAQIAAVMFAGQRKGLDFTLAEGQEVIVSGTVDVYEKDGRYQLYAKEIKRE
                     GRGDLFLRFEKLRDELEEMGMFDGCYKQPIPKYATRIGIVTASTGAAIRDIMNITSRR
                     NPYVQLILYPALVQGEKAKYSIVQGIKTLDQMGLDVLIVGRGGGSIEDLWAFNEEMVA
                     RAIFECHTPVISAVGHETDVTIADYVADLRAPTPSAAAELAVFDYKQFEETVAAYKLL
                     LDKAAKRKLERARFRLEQCRMQLKLKDPGRMVNDRRQRLADYEEILDRKIKEALKESK
                     RLLEKQEERLNNSMREKLNLQKHRLALDASQLEGLSPLKKLSAGYGFVQKESGEAIRS
                     VKQVTAGDPVRIYVADGYAKALVTDTKNSRPGRKG"
     gene            19754..19987
                     /gene="xseB"
                     /locus_tag="ACZPCL_03965"
     CDS             19754..19987
                     /gene="xseB"
                     /locus_tag="ACZPCL_03965"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR1161679.1"
                     /translation="MAGRKKAEEAAPEKTAGIEENFARLEEIIKKLETGENSLEETFA
                     GYEAGMKLVKSLSSQIDKVEKQIQILSEGEDNE"
     gene            19980..>20369
                     /locus_tag="ACZPCL_03970"
     CDS             19980..>20369
                     /locus_tag="ACZPCL_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR1161680.1"
                     /translation="MSDNTEFRKELEERTKQINEQIESYLPEEKGHQKTIFEAMNYSV
                     KAGGKRLRPLFMQEICRLFTGEVQPVVVPFMAAIEMIHTSSLVHDDLPCMDDDMMRRG
                     QASTWAEYGEDMGVLAGGPHGPCHEGGG"
BASE COUNT         6224 a         3789 c         5343 g         5013 t
ORIGIN      
        1 agcagaaaaa tccagctttt tttacagtgc ctgcgccagg tgcttttaga aatggaacag
       61 gtggagtttg agatttacat atagaagaat atattaaaga atatattaaa gatattcgtg
      121 atccgcttat ttttctttga aaattggtgt ggtggatata aaacaggagg atgtaaagcg
      181 catgataaac agagatgaca tgctggagct gaccaggcgt atgacaccgg ccagagcttc
      241 cgtaggccgg atcgcagggg cctattttga tgaagaaggt tatgtggatg gtacgtttaa
      301 tacgaatttt ctgaagctgt cagccagtga gcgtaccaga aatttaaata tggcgaaaac
      361 ggtgctgatc tctaagacaa atgagcagtt gaaggaatac agaattacaa gagagatggg
      421 aaagccggga agtgtatggc agctgcttga tggcgtaaaa gagtcggaaa tgaaaaatga
      481 tgcctatatg gacctgttct atgaagtact gggagagaaa tatcatccgg gatacccgtt
      541 ttcctgtttt atgtactacg gtcagtatga tgtgccggta aagggaacgg ataaggagtg
      601 gatggaaggc tctgaggagg tttattcata tctgttgtgc gttatcagtc cgttgacagg
      661 tgaatatgag cctggggcac cggagtttgg atttttgtat ccggctttta aggaccggag
      721 tacaggctgg gagtttgtaa atgtgtatga gaagctgccg gaacgggtac accgggaatg
      781 gatggaatgg ctgtttaaat aaaaaacaat aataaataaa ataatgagaa atgaagtttg
      841 tgttaaaagg agaaggggaa aatgacaaag gttacaacaa agaaaaaact ggctgtttta
      901 ggaaatggtt ttctggcggg gatcattgta ggggcttata ataagggact tttagaggaa
      961 tatgagctga ccggtatatt gggacggaca aaggaaaaga cagaagcact ggcagaaaaa
     1021 ggcggctgcc gtccatgcag cactttagaa gaactgctgg aagacaagcc ggattatgtg
     1081 gcagaggcag cttctgttaa ggctgtccag gattatggta tgaaaatatt gtccggcggg
     1141 gcagatatga tcttgttatc tattggtgcg ctggcagatg aaacatttta cagagagatc
     1201 agtgaatgtg caaaagaaaa tggaagaaag atccacattg cttccggtgc catcggcggt
     1261 tttgatgtgc tgcggaccat ctctcttatg ggacaggcac agacttcttt ccgtacaaaa
     1321 aagggcccga aatccctttc cggaacccct ctgtttgaag aaagccttat gacagataaa
     1381 gaagaaaagc aggtatttca cggaagcgca aaggaagcca tttctattct gcctactaag
     1441 gtaaatgtag caattgcagc atctttagca tcttctggcc cggaaaatgt agatatgagc
     1501 attttcagtg tcccggaaat ggtaggagat gaccacaaga ttacagccca gataccggga
     1561 gtaaaggcag agctggatat ttattccagt accagtgcta ttgcgggatg gagtgttgtg
     1621 gcagtgctgc gaaatctggt ttcaccgatc gtattctaaa taacaggaat agaaaaaggg
     1681 aagctgacat tgcatcagct tcctttttaa aaaccttatt ttaagccatt tcttagaact
     1741 taccagcctt tgcagcttcc tcgatggaaa cagcaactgc aacagtagca cctaccattg
     1801 ggttgttacc cataccgatc agacccatca tttctacgtg agcaggaacg gaagaggaac
     1861 cagcgaactg tgcgtcagag tgcatacgtc ccatggtatc ggtcataccg taggaagcag
     1921 gacctgcagc catgttgtct gggtgaagag tacgtcctgt accgccgccg gaagctacgg
     1981 agaagtactt cttgcctttt tcgatgcatt ccttcttgta ggtaccagct acaggatgct
     2041 ggaatctggt tgggttggtg gagttaccgg tgatggaaac gtcaacgcct tccttccaca
     2101 tgatagcaac accttctgtt acgtcgttag cgccgtagca gttaaccttt gctcttaagc
     2161 cgtcagagta ggactttctc cacagttctt ttacttcgcc tgtgtagtaa tccatctcag
     2221 tttcaacata tgtaaatccg ttgatacggg agatgatctg agcagcgtct tttcctaaac
     2281 cgtttaagat aacgcgcaga ggtttctgac gaactttgtt agccttttca gcgataccga
     2341 tcgcgccttc agcagctgcg aaagactcat gtcctgctaa gaagcagaag cagtcagtat
     2401 cttcttccag aagcatcttg cccagattac catgtcctaa accaacctta cgctggtcag
     2461 caacagaacc tggaatacag aatgcctgaa gaccttcgcc gattgctgca gcagcgtcag
     2521 cagctctctt gcagcccttc ttgatagcga tagcagcgcc tacagtgtaa gcccagcaag
     2581 cgttctcgaa gcaaataggc tggatcttct taacctgagc ataaacgtcc aggcctgcat
     2641 cttttgtgat cttctcagct tcttcgatag aagcaatgcc atagctgttt aatacagaat
     2701 tgatcttatc aattcttctc tcatatgatt caaataatgc catcttctta atcctcctaa
     2761 ttggtcctca tcattcttta cgtgggtcaa cgatcttaac agcgtcagca acacggccgt
     2821 actgtccttt agccttttcc catgcagtag taggatcatc acccttcttg atgaaatcgg
     2881 tcatcttgcc aaggtttacg aactggtatc cgatgatctg atcatcagcg tctaatgcaa
     2941 taccggttac atagccttct gccatttcca gataacgtgg acctttctta agagttccgt
     3001 acatagtacc aacctgagaa cgcagtccct ttccaaggtc ttccagacca gcacctacag
     3061 gaagtccgtc ttcagagaat gcactctgag ttcttccgta aacgatctgt aagaacaact
     3121 ctctcatagc ggtattgatc gcatcacata caaggtcagt atttaatgct tccagaacag
     3181 ttaaacctgg aaggatctca gctgccatag ctgcggagtg agtcatacct gaacatccga
     3241 tggtctctac cagagcctcc tggatgatac cttcttttac gttcagggtc agcttacagg
     3301 caccctgctg tggagcacac cagcctacac cgtgtgtcag accggaaatg tctttgatct
     3361 cctttgattt tacccatttt gcttcttctg ggattggagc agcgccatga tgaacgccct
     3421 gtgcaacttt gcacatttct tctacttcct gtgaataaat catgtgttaa aactcctttc
     3481 aaagatactt gtatgattgg ttgtgtctgt aggaacagac atgtccgttt gtttaaaccg
     3541 gatgcctttg ttaaaattat cacggcacac ctatgttcat tttctcacat aatatgacat
     3601 tttacaagca aaattttctc atgtgagaaa caagtttttc atgtaaatgt actaaaaaaa
     3661 atacaggata acaggaaaat gggtataaaa aagactgcta taaagtgaaa aattcatttt
     3721 acagcagttc tttatctcca gccggagcgt gttcccggga taatcagtgt gagaccggac
     3781 acctccgata cgggcgaaac cgcactcctc ccagaactgg cgacttcctt attataaaac
     3841 tctaaataaa aaaatacaag ggtggaataa cctgggaaag ggaacgtaag agaagtagtg
     3901 gaaataagat cgtggaaaat gaagaaatgt gtaaaaatgt ctgtaaaaag ggggatggcc
     3961 cggagtactc tgcgtagcag gtacttccgg gcccgcgtca tctctgactg gtttaaaata
     4021 taccatgacc cggtttaaaa tgcaatggaa gtcatagaaa cgtaatgaat tgtaatattt
     4081 cttacaaaaa acgatagaaa gagttataaa agatagatga aaaatatttg aaaaatctga
     4141 ggttttgtta tatactacat attacgatag cggcagtatg tctttagcga tgtatccgct
     4201 aaatatacaa ataaggatgg ttttactatg gcattattag aaacatggag aaatctggca
     4261 tatggtgacg gcctggatga taaaaaaaga gaagagctgt ggtccggata tttccagatc
     4321 gagaagggaa tttatgagca gatcctttcc cagcctgagc aggtaatcac aggtactgta
     4381 aaggaactgg ctgagaagta taacactgag atcctgatca tgaccggatt tttagatggt
     4441 atcaatgaaa gcttaaaggg atatgagaac ccaatcgata ctatggaaga agataccgaa
     4501 gttaagatcg agatcgatcc tgagaagctg tactacaaca tggtagaagc taaggctacc
     4561 tggctgtatg aacttccaca gtgggatgcg atcctgactc cagaaaagcg taaagaactg
     4621 tacaagaacc agaaggcttc cggtactgtt cgcaaggcaa agaagatctt cccaaatgat
     4681 ccgtgtccat gtggaagcgg caagaagtac aaaaaatgct gcggtaagaa cgcttaagca
     4741 atctgaaatg tgcgcctgtc cggactcttc cgggcaggta ttttaatgtt acagtccaga
     4801 tcctgctggt tcggagaaac tgtaatattt taatgggaga agaaaatgga agcatataca
     4861 agctttgcgc aggtctatga tctgtttcag gataatattc cttatggaga atgggcagat
     4921 tatttaaaaa gcctgttaaa tgaatatgga gtaaaagacg gactggttct ggatctgggc
     4981 tgcggcactg gaagcatcac tgagctttta gcaaaggccg gatatgacat gatcggcgtg
     5041 gacaattccg aggacatgct ggagatcgcc atggataagc ggagcagatc gggactggat
     5101 atcctgtatc ttttacagga tatgcgggaa tttgagcttt acggtacagt aaaagcggca
     5161 gtgagtatct gcgattccat gaactatatc ctggaaaaag aggacctggt acaggtattt
     5221 aaactggtaa ataattatct ggatccgtca ggtatcttta tttttgacat gaatacagaa
     5281 tataagtaca cccatctttt agcagatggt acttttgcgg aaaacagaga agaaagcagc
     5341 tttatctggg agaatttcta tgatgaagag gagcggatca atgaatatga cctgacttta
     5401 tttattaaag aaggggagct gttccgcaag tttgaggaga ctcattatca gagatgctat
     5461 tctttggatg aggtgaaaga ggcagcaaaa gaggcgggaa tggattttgt ggccgcctat
     5521 gatgcgttta ccagggaatc tgtaaaggat gacagtgaaa gaatttatct tgttttccgc
     5581 gagcgtggga aataagaagg accattaacg agcagtcagc ctgcgtaagc agacagtaaa
     5641 ctgcgatagc acgggactgc gggtataaaa ataacaagga gagaacaata tggcagatta
     5701 tgtgatcaga gccactgcgg cagaaggtca gatccgtgcc tttgcggcaa ctaccagaga
     5761 tctgactgag actgcaagac aggctcacaa tacaagtccg gtagcaacag cagcgctggg
     5821 aagattaatg acagcagctg tgatgatggg ctatgacatg aagggagcag atgaccttct
     5881 taccataaag atccagggcg atggccctat tggtggtctg gttgttaccg ctgactctca
     5941 tggagaagta aaaggttatg cttttaatcc gggggtcatg cttccgccaa atgataaagg
     6001 caaactggat gtaggcggtg ccttaggcgt aggcgtttta agcgtgatca aggatatcgg
     6061 cttaaaagag ccttatgtgg gacagaccat tctggtatca ggagagatcg cagaggattt
     6121 aacatattat tatgcaacct ctgaacagac cccttcttct gtggcactgg gcgttctgat
     6181 gaataaggat aatacggtcc gtcaggcagg cggatttatc atccagctga tgccaggtgc
     6241 ttctgatgca gttatctctg ctctggaaga gaaaatcaaa gagatccact ctattactac
     6301 attattagat gtgggaaata caccggagac aatcttacag tacattttag gagacttagg
     6361 actggaaatc aatgaaaagc ttcctgctaa gtttacctgc aattgcaata agagccggat
     6421 cgagaaggcg ctgatcagcg ttggaaagaa agacatccag gaaatgatcg atgacggtaa
     6481 gccgatcgag gtaaactgcc atttctgcaa taaaaattat acctttgatg tagaggaatt
     6541 aaaagagatc ctggaaaagg caagaagata aaatacacac aaaaggagcc agtcatgcga
     6601 ctggctcctt tgtaatcatt tgaaattcac gtataactta cttcaactat ctatcaattt
     6661 gatatatgtt tgaaaaaggt acactgttgt tcattgatta taatttggtt acgttagtag
     6721 cctgaggtcc cttagcacca tttactacgt cgaactcaac ttctgcgcct tcgtctaaag
     6781 acttgaagcc ttccatgttc agacctgagt agtgtacgaa tacatcgttg ccctgctcgt
     6841 cagaaatgaa tccgtaaccc ttctggttgt tgaaccactt aactgtacct ttcattacga
     6901 tacctccaaa aaatataaaa aaatgttgag ttgcaagtaa tttcgcaact gtgattagaa
     6961 tatcacaaat taagggcggt gtcaaacctt ttgtgcctaa gaaaataagt attgagagga
     7021 agaaaaagtt ctggtacaat aggcaaagaa aaaaatttaa caatgtttaa ttgagagttt
     7081 ttttgagtta gagaggattt agaaatgtct gaaaatgaaa tgaaaatgcc gaaaataaag
     7141 ctggtcaata ttgtaaaaca tttctgtacc attaccagac accgtctgtt agtgtgtaag
     7201 cactgcttta agatcggatt atactggcag ggactgacac atgacctgtc aaagtacagt
     7261 ccggaagagt tctggacagg agtccgttat taccaggggg accggagccc taatgcggca
     7321 gagcgtgagg cgattggttt ttccaaagcc tggcttcatc acaagggacg gaacaagcac
     7381 cattttgaat actggatcga tgtttcttta aataaagaag aaggtctggt gggaaataag
     7441 atgccggtga actatgtggc agagatgctc tgcgaccgga tcgcagcctg cgaggtgtac
     7501 cgtgggaaga attacaccag cgcagcacct atggaatact atgaatttac aaagaaatac
     7561 attaccattc acccggagac cagggcgctt ttggaaaagc tccttacgat tttaaaggat
     7621 ggaggggaag agagcgctta tgcttatatc cgtaagctgt taaaaaaggg aagctattaa
     7681 aacaggcttc cctttttcgt gcttattttg ttgtgtttta agctgttttg gactgcttac
     7741 tcttctggaa acaggttctc ttcgataatg gcaaccgcat cacggatgca gtccggacat
     7801 gcgcggagga cttttccggt gtctacgccc tttaattcac ggcatacgat ggtggagttt
     7861 ttctccttga aagctgcgat gcaggctttg gatgccttat aggaagttcc cttggagttt
     7921 ggcttttcca ggtctgcagt gctgatcttt aaaccggcta aaacggctgc tgcggagata
     7981 gcgccacagg ttccttccat accgcccatt ccaaggccaa ggccctcagt tactttaaac
     8041 agagtctccg ggtcaatatc taccttatct aaaaatgcga aagctacaga ctgtgcgcag
     8101 ttgtaacctt tgttgtgaca ggcaatggct ctgtcggctc ttgactcttt tgtgatctct
     8161 gacataatca aattctcctt ttaaaattaa aatttggaaa tttttgtaaa ataatgttgt
     8221 cctatttcca ggcagcttat atgaatgata aaaccgccaa gaagaacact ggcaaggtaa
     8281 agacagtttc ctgcaaatgt tccgatcacc agggagatct ggtgaaagac gatctgcccg
     8341 tttggataat agggactgat gtaaaacata tccgcattgc cgtaaggatg ggataataca
     8401 tttacagctg tggcaatggc agtacagaaa agaaacaacg ggatcgtatg cagatagctt
     8461 tttggcgttt ctgttttcag gttataaaat gcacagataa gccccataaa aataaggttg
     8521 aaatgccata aaaagccgtg gacggtcatg gtccagtaag gatgcatcag tccgctgggt
     8581 tcagccagtg ccatgatccc gccaagaaga ccgaaatcct ggataaaaag gtaaaagaca
     8641 tttcttattc gggcaggaac catgggaatg aaaagtccaa ggtacatggg aacactgcac
     8701 agctggaatg gaaaatacca ccagtcatag gttctgccat tttctatata atagataaat
     8761 ccctgtttat ataattctgt cagccctaaa aataagccgc ataaaaaaag aatggtatcc
     8821 ggtccggctt ttctttccgg aaaagagaac ttccgggcaa gaaaaatgct taaggataca
     8881 ccaaggacgg tcaggaccag atgaaaagct ccatagggct taggagtatc catggtccag
     8941 gcggttgcct gaaaaaaagc ttcagccata taaaaaccac cttctgaaat tgtatatctc
     9001 gttaaagatt atacaacatt caggtgagat tgtccatgaa gggagaataa aaagagggaa
     9061 gcctgcgcag tctaagaaag agaaaaaaga ttggatacag tatgggaaaa ttccaggagg
     9121 gcaaaaaatg gcagggatag taacagcgct tttatccggt gcattgatga gcgtacaggg
     9181 cgtatttaat acaggtgtga caaaacagac cagcgtgtgg gtggcttccg gctgggtcca
     9241 ggcaacagcc ttgattgcct gtgttgttat atggtttttt actgggagac cgccggtgcg
     9301 gggactcttt tcggtttctc ccatctacct tctttccggc ggtatcatag gggcggtgat
     9361 cacctataca gtgatccgca gcatgcagtc tttaggaccg gcacagactg cccttctcat
     9421 cgtagtggca cagatcatca cagcatatgt gatccagctg tttggatggt ttggcacaga
     9481 aaaaatgcct tttgaatggc ataaactttt aggagcagct gttgctgtgg cagggatcat
     9541 tatttttcag agataaagcg aaaagtcaaa aaaagacggg aaaatgttag aattaccgct
     9601 tgtatttaaa ttgcctttcc gatagaatag taatagcagg ctgagatggt attccatgga
     9661 ggaatatcgt tgaagagaac attatctttg aagaagaaaa agcagatagc tgcggccctg
     9721 gctgcagctg tgtttgtctg tggcggttgc acaggaaatc tgaaagaaga acaggatgag
     9781 atcctgcttg aaaccgtggc tgaaagcagt gaaaacctgg aacatgcaga tgatgcagaa
     9841 cagtcagaag caggagaaac attaccggaa acagctgaac cccaggaaac agcagaaaca
     9901 agattgggat ctacaatcta cgttcatgta tgcggtcacg taaaatatcc aggcgtttac
     9961 cagatgcagg aaggcagcag agtctatgaa gccattgata aggcaggtgg aatgttggaa
    10021 gaaggtgccg gtgatgccat gaatatggcg gctgtgctgg aagatggtat gaggatcatg
    10081 gtaccggatc tggcccaggc agcccagatg atagaacagg gcgctgtttt tgatcctggt
    10141 tcatctgcaa gtgtttctga ccggaaaagt tccggaaagg tgaatttaaa cagggccgat
    10201 gtgaaagaac tgatgacatt aactgggatc ggccagtcac gggcagaggc gatcatacgt
    10261 tacagggaag aaattggcag ctttgagaga atcgaagatg tgatgaaagt gtccgggatt
    10321 aaggaaagcg cttttaataa gatcaaagat gatattactg tatagttgat agagcggttg
    10381 ctggcttgcg gtgcatggac agtgacgata aagagataga tatagataaa ggggagctga
    10441 aaaatggcac gggtattagt agtagatgat gaaaaactga tcgtaaaagg gatccggttc
    10501 agtctggaac aggatggcta tgaggtagac tgcgcctatg acggagaaga agccattgaa
    10561 atggcaaaaa agacagaata tgatattgta ctgttagatg taatgcttcc caagcacgat
    10621 ggatttgaag tgtgtcaggc gatccgtgaa ttttcagata tgccggtgat catgctgact
    10681 gcaaagggca gtgatatgga taagatcctg ggactggaat acggggcaga tgattatatc
    10741 agcaagccct ttaatatcct ggaagtaaag gcaaggatcc gtgccattat ccgtcgcagc
    10801 atgaaggcaa aacgccagaa aaaggaagaa caggccaata cttacattgg ttccggcgac
    10861 ctgaagatgg acacagaagg aagaagagtg tttatcggtg aaaaagagat caatcttaca
    10921 gcaaaagaat ttgatctgtt ggaactttta gtgcgcaatc caaataaagt atacagccgt
    10981 gaagccctcc ttaattatgt atggggaaat aaggctatgg acagcggaga tgtaagaact
    11041 gtggatgttc atgtaagacg gcttcgtgaa aagatcgaac caagtccaag tgaccctaag
    11101 tttgtacata caaaatgggg cgttggttat tatttccagg cgaaataagg aaaaacagaa
    11161 ggaaaagaaa gtcagtgaag attacattag ttacagtagg aaaaataaaa gaacgttttt
    11221 ttgaggatgc cattgctgaa tacagtaagc gtttgggaag atattgcaag ctggagatca
    11281 ttcaggtggc agatgaaaag acgccggatg gggccagtga aaatgtggag cggcagatcc
    11341 gggaaaaaga ggggcagagg atcctggccc agatcaaaga cggtgcttat gtgctggcac
    11401 tggcaataga aggaaaaatg ctttccagtg aggaactggc aggaaagata caaaaactgg
    11461 gagtagaagg caaaggccat ctggtatttg tcatcggtgg atccctggga ctttccaaag
    11521 aggtgatgga ccgggcagat tatgccctca gtttctcaaa aatgacattt ccgcaccagc
    11581 tgatgcgggt gatacttttg gagcagatct acagaggatt taagatcata gcaggagaac
    11641 cctatcacaa gtaaaagaat atgttaccta aaggtgacag caccataaaa aggcagttgc
    11701 tacagtgaaa tagtaaaatt cactttggca gctgcctttt tgtgcaatga tgaggcagtt
    11761 tggcgcgatt caatgcaagc gatttattta cctttgttcc agacagcatg tcataaaatc
    11821 atgttgtcct catatactgg agttagaggt acagaaaaat ggcagcaaaa acagaaaagg
    11881 gattaaagca ggagatcgta aatttgtttc ctgtaaggct cagacagata ttagaagcac
    11941 ttcccctgga ttttgcccgg ttagaagaga tccgcctgcg ctgcggtcag cccattctct
    12001 ttcgcatagc aggaaaggaa atgggaatta cgggttctgg agatctgaca gaactgggat
    12061 cctcaggaaa actggaaaac tgggaaaaac tggaaaaaat aagggaaaaa gaattaaaag
    12121 ataccctgga gatcatggga ggtttctccc tgtatgcagc agaagaagag ctgcgccagg
    12181 gcttttttac agtccggggt ggtcaccgga tcggggttgc agggagagtg atattaaaag
    12241 acaggcagat cacgggactt aaagcagcat catttttaaa tgtgagagtg gcccatgaga
    12301 taaaaggatg tgcagatcag gttcttccat ttttatatgt aaatggaaaa tttgtgagta
    12361 cattgattgt ttctccacct ggatgtggaa aaacaacact gctcagggat ctgatccgtc
    12421 aggtttctga tggaagcagc cagaaagcag gaaaatgcag ctttgccggt gtaaacgtag
    12481 gggtggcaga tgagcggtca gaactgggag cctgttatca gggcgttcca cagaatgatc
    12541 tggggatgcg taccgatgtg ctggacggat gcccgaaaag cgagggaatg ctgatgctga
    12601 tccggtccat ggcgccggct gttgtggcag tagatgagat cggcggaaaa gaagatatca
    12661 aagcagtaga atatgtaaga aactgcggct gcgccctggc agcaacggct catggaaatt
    12721 ccctggaaga gttaaaagaa agagccggtt ttcgggaact tttagaggaa aaaacttttg
    12781 agcgtctggt gtttttaagc agaaagcggg aatgccgggg aaaagtggaa gtggtttatg
    12841 acagtggttt taaccggttg tattccggga gaacatttta acagaaagga ggatttctat
    12901 gtttctaaaa tggacaggcg ggctgctgat cctttttgca ggttcgggaa tgggcgtatg
    12961 gctggcatgg ggatataaaa agcggttgag cactttagag aacctgagaa ggatgatcta
    13021 ttgcctgaaa ggggagatcg tttacagtca tgcaccttta gaggaagcct ttgagcgcat
    13081 cggcaaacgg gaaaaaggtg ttttgggaga actttttaca cagactgcag aaaagatcgg
    13141 ctgtcataac ggagagacat tttccaaaat ctgggaacag acaatagaag aaatggaaaa
    13201 aaatggaaaa ccactgtttc tggaaaaaga ggacagggaa aaactccttt ctttaggggg
    13261 acagctgggc tatctggata cccagatgca ggagaggacg ttacttttgt atttggaaca
    13321 gctggaactt tctgtttcaa gactccgcgg agagatccgg gaaaaatgcc ggctcagtac
    13381 agtcttaggg gtgatgggaa gcctgttttt agtgattgta atgctttagg ggtagaacgt
    13441 aattatggat gtaaatctca ttttcaggat agcagcagtg gggatcctgg tttctgtgat
    13501 ctgccaggta ctaaaacaca gcggaagaga tgaacaggca tttcttacca gtctggcggg
    13561 gctggtcatg gtgctgttct ggatgatccc gtacatatat gatctgtttg aaaccatgaa
    13621 gcagctgttt ttgctgtaag ggaggaatgg ctatgaatat gctgactttg ggggcagccg
    13681 ggatcgcggc ggtccttctg gctgtgtgga tgaagggagt aaaggcagag tatggtattt
    13741 ttgtggtgat cgcagcccag cttttcttcc ttttttatgg tacagggcgg ctgggagaga
    13801 tcctggatac agtcaggcag ttggagcagt acataaagat ccggccggtg tatctcataa
    13861 cacttttaaa gatgaccggt attacctaca gtgcagaatt tgcctccggg atctgcaaag
    13921 acgccggata tgcctctttg ggaaagcaga tcgaaatatt tgggaaactg acgatcctgg
    13981 cagtgagtat gccggtcctt ctggcattat ttggcacact ggaccagata cttacatagg
    14041 ggatctgaaa aatgagaaaa caggcaaaca ggtggctggt ggcagtattt ctgctttttt
    14101 ttattttttc aggcattcat atgacggctt ttggggaaga acctgcaaag gatcccctgg
    14161 cgcagttgga agaagaaatg gggatcgggg aagagatcca gaggatccag gaatatctgg
    14221 atgggctgcg gccagaggga agaacggtct cccttacaga tctgatgcag gtcattattc
    14281 agggaaacat gaaagaaatc gtaaatacag cagggaaact gctggaagat gccctgtttt
    14341 cccaggcaaa ggagggacag gcccttttag tacaggtagc ggttttaggg ctgaccgggg
    14401 cggtattttc ccatgcagct tccgctttta aaggcagcca gatcccggaa actgcttttt
    14461 acatgatcta tctgctgatt tttacctgtc tggcaggaag ttttctgtcc agtatccaga
    14521 tcacagccca ggtattagac cagatcctgg agtttgtccg tctgcttatg ccgacctatt
    14581 ttattagcgt ggcctttgca gggggaagta ccagtgctgc tgctttttat gagatcgccc
    14641 tgggagctgc ctgggcggtg cagtggctgt gccgtcaggt atttctgtcc attgtgcgtg
    14701 tgtatggcct gctggtactg ggagatcaca tgatggaaga gccatttttc agcaaaatga
    14761 cggagttaat ggaaaaaatc gtatcctggg gattaagaag tatcctggga ctgacaatgg
    14821 gacttcagat ccttcagtca ctggtacttc cctatgcgga cagtgcaggc agatctgcga
    14881 tcatgaaatt tgcagaaatg ataccggggt taggacaggc aaccggtgca gcggcaagac
    14941 tggtgtttgg aagcagtgta ctgatcaaaa acagtctggg agcagcggcg gtcctgatcc
    15001 tggcagtgat cacgttggta ccggttgtga agctggctgt tttaatggtc atgtatcagg
    15061 gtgcagcggc tcttttgcag cctgtttgtg acaaaaggat catttcctgt atccagggga
    15121 tggcggcagg ccatggactg cttttaagga tcacccttta ttcccttttc ctgtttatcc
    15181 tggtcatagc cataacctgc gcaggcacca atgtcactta tctggctgcg taacaaacag
    15241 taagcctgaa atagtaagcc tgaaacataa aaacaggcag aggaaaggta tgggagagat
    15301 tttattagaa tgggcaagga atattatatt ttttatggta ttcctgtctg tgatcagtca
    15361 tttgctggca gatgcgtctt atgaaaaata tatccgtttt tttgcaggga tcgtgctgat
    15421 cctgatcaca gtcagcccct taaaaggcgg tctggacttt caggagaaag caggaagatt
    15481 ttttgaagaa ttttcttttt ttcaggaaaa ggaacaggcg ggaaaagcac tttcaaaggc
    15541 agaccaggaa agaatgggtg catttcttgc ggaatataaa aaagaagcgg aagcgcggat
    15601 ccgggaaacc atgcaggcgg aaggcgttat ctgtagccag gcagaggtgg aaatagaaaa
    15661 agatgaaaaa agcggggaat acggaaagat caggaagatc attttacatt taaaagagga
    15721 aaaaatgaca tcaccacagg acgaagaaac gaaaatggta aaagaaaaag caggggaaga
    15781 agtatctgta aaagtggaaa ttccccctgt tataaccaaa gaaaagcagg aaagaccagg
    15841 cactgagtcc aaacaggagc agcagttaaa aagaaaggtg gcgcagtatt atggactgga
    15901 agaagcttgt atcgaaattt ggtgggaaga tgaccaggga gaaatggctg tttctgcttc
    15961 tgttaggagt gatcctgatg atcctgtcta tgccatttcc gggaagcagc agtaaaaaga
    16021 caggtcagac gaaaggggaa aatgtttcac tctgggagaa aaacagttcc ggtgacggac
    16081 agcagacaga ggaaaacata gttcaggaac ctgtacaggc agcatcggca aaaccggccc
    16141 aggattatga aaaggagctg gaagatcgga tcagccagct tttaaagtca gtggaaggag
    16201 tgggaaaagt agacgtaatg gtagttttaa gttcttccgg tgaaaaggtt ttccgcacag
    16261 accggtctga aaataccagt accaccaaag aaaaagacgc ttccggcgga gaaagggatg
    16321 cagtgagcag ccagttagaa gaaaataccg ttttttccca gtctgcaggc cagaatacag
    16381 gaaacggacc tttgatcgaa aaagaactgt atccgcagat ctccggtatc gtgatcagtg
    16441 cccagggagg tgatctgccg tcggtccagg ctgaaatttc ccaggctatg gaagcattat
    16501 ttggactgga accgcataaa ataaaagtgt taaagcgggt ggaataaaaa ggagtgcggc
    16561 atatgaagat cagaaatatg aagcaggttg gcgagcgggt aaaagagata aagatgaagc
    16621 gtctgtttcg gcgaaaccag atcattatca ctacattggc tgtgatgatc gctgctgccg
    16681 ggtatctgaa ttatgcagga aaaagagatc tggaagcagg gggacaggtt gcggatatgg
    16741 gaagcctgga gctttcagat gaagatctga tggcagaaaa tctggcagca cagtcagaga
    16801 cagtccagga cattttaagt ctggacaatg atccaaatga tacagaggta gctgaggcac
    16861 agacagaagg aacggatctg gcccaggccg gcagtgagaa cggtgaacag tcaggtatgg
    16921 cggatacaga ggagcagacc acgggaaatg atgcgcctgc aggctatgaa aatccggggg
    16981 aagcagtcct taccagcggt atgagcgtag cagattacat agcaggagtg cagcttagca
    17041 gggaacagat ccgggctaaa aacaaggaaa ccttaatgag cctgatcaac agcaccagca
    17101 ttgatgaagc agccaagcag caggcgatcc aggatatgat ccgccttacg gaaatatcgg
    17161 aaaaggaaaa cgccgcagaa acgctgctga tggcaaaagg attttcagat cctgtagtca
    17221 gtatcacaga tgataaagtg gatgtggtga tccatgcttc ctctattaca gaccctgaaa
    17281 gagcccagat cgaggacatt gtaaaacgta aagcagaagt aggagccgat cagatcatca
    17341 ttaccctgtt aaatatggca gactaaaaag aactatacat atcctgcgcg atttggtata
    17401 ataatgagta acagttttat gatcacacag agcaggaggt aagggatttt ggcagaagaa
    17461 agtcgcagta cacataaatt atatgaaaaa gacaagatcg gtgaagtaca gatcgcagat
    17521 gaagtggtag cgatcattgc aggtcttgct gctaccgaag tagaaggcgt agattccatg
    17581 gcaggaaata tcaccaacga actggttgga aagcttggca tgaagaattt aagcaagggt
    17641 gtaaaggtgg atgtgacaga agaacacgta tctgtggatc tgtctttaaa catccgttat
    17701 ggttataata tccctgcagt cagcgagcag gttcaggaaa aggtaagttc cgccattgaa
    17761 aatatgaccg gtcttactgt tcttgatgta aacattaaga tcgctggcgt gaatatggaa
    17821 gaaagctaaa agcatttacg aaagtgcgcc ctgcaggtat ggaaggaggc ttctttactt
    17881 gcagggcctt ttgtacattt tttgttgttt ttatgcaccg cctttggtat aatcacaggt
    17941 agtggaaaag aattaagaga aagaggaaaa atgaaatgac cagaagagag ttgagagaac
    18001 attgttttaa aatgctgttc tctgtggatt tttatacaac cggtgaagaa gccaaagccc
    18061 agatgcagca gtattttcag tctccagagg aggatgatac agcagaagac ggcagccttc
    18121 aggtagtgca taaagtagca gccggagaaa aagacagaga atatctcagt acaagaactg
    18181 ctgatgtagt ggataaaata gaagagatcg atgccaagat cgacgaagta gcagctggct
    18241 ggaagacaaa gcgtatggga aaggtagaac ttaccatcct gcgtcttgcc gtatatgaga
    18301 tgctttatga cgataccatt cctgagaagg tgtctgtcaa tgaggcagta gagctggcaa
    18361 agaagtttgg cggaaatgag tcccctgcgt ttgtaaacgg cgttcttgca aaattcattc
    18421 caaaagaaga aaagaaagaa gaaagcaagg aagatcagga agcataaatg ggaagcgtgt
    18481 ataccgtatc tcaggtgaca tcttatatta aaaatatgtt cacccaggat tttgcgctga
    18541 acagagtctc tataaaagga gaagtgtcca actgcaaata tcatacatcc ggccatattt
    18601 attttacatt aaaagacggc ggcgcacaga ttgccgccgt catgtttgca ggacagagaa
    18661 agggcctgga tttcaccctg gcagaaggcc aggaagtgat cgtatcaggc actgtggatg
    18721 tatatgagaa agacgggcgt taccagctct atgcaaagga gatcaaaagg gaaggcagag
    18781 gagatctgtt ccttcgtttt gaaaaactga gggacgagct ggaagaaatg ggtatgtttg
    18841 atggctgcta taagcagccc atccctaaat atgccacccg gatcgggatc gttacagcgt
    18901 ccacaggcgc ggccatccgg gatatcatga atattacatc cagaagaaat ccctatgtac
    18961 agctgatcct ttatcctgct cttgttcagg gggagaaggc aaaatacagc attgtccagg
    19021 gaataaaaac actggatcag atgggactgg atgttctgat cgtaggacgg ggcggcggtt
    19081 ccatagaaga cctgtgggca tttaatgaag aaatggttgc aagggccata tttgaatgtc
    19141 atactccggt gatctccgct gtaggacatg agacagatgt gaccattgca gattatgtgg
    19201 cagacctgcg ggctcctacg ccatcggcgg cggcagagct ggcagttttt gattataaac
    19261 agtttgaaga aacggtagct gcctataaat tgcttttgga taaggctgca aaacgaaaac
    19321 tggaacgggc ccgtttccgc ctggaacagt gccggatgca gctgaaatta aaggatccgg
    19381 gccgtatggt caatgacaga cggcagcgtc tggcagatta tgaggagata ttagaccgga
    19441 agatcaaaga ggcgttaaaa gaaagcaaaa gactgttaga aaagcaggaa gaacgtctga
    19501 acaattccat gagggaaaag ctgaatttac agaagcaccg tctggcatta gatgccagcc
    19561 agttagaagg tttatctccg ttaaagaagt taagcgccgg atacggtttc gtacaaaaag
    19621 agtcaggaga agccataaga tctgtaaagc aggtaacagc cggtgatccg gtccgtattt
    19681 atgtagcaga tggttatgca aaggcgcttg tgacggatac gaaaaacagc agaccgggga
    19741 gaaagggata aatatggcag gaagaaagaa agcagaagaa gcagctccgg aaaaaacagc
    19801 cgggatcgag gaaaatttcg cccgactgga agagatcata aaaaaactgg aaacagggga
    19861 aaattcactg gaagagacct ttgcagggta tgaagcagga atgaaacttg taaagtcttt
    19921 aagcagccag atcgataaag tagaaaaaca gatccaaata ttgagtgagg gtgaagacaa
    19981 tgagtgataa tacagaattc agaaaagaac tggaagagag aacaaaacag atcaatgagc
    20041 agatcgaaag ctatctgccg gaagaaaagg gccatcagaa aaccattttt gaggctatga
    20101 actacagtgt aaaagctggt ggcaagcgtc tgcgtcctct gtttatgcag gagatctgca
    20161 gattatttac aggagaagta cagcctgtag ttgtaccatt tatggcagcc attgagatga
    20221 tccatacttc ttccctggta catgatgatc ttccatgtat ggatgatgat atgatgcgca
    20281 ggggacaggc aagtacctgg gctgaatacg gcgaagatat gggcgttctg gcaggagggc
    20341 cacacggccc atgccacgaa ggaggaggc
//
LOCUS       DBMDUA010000029        19814 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1673_length_19814_cov_3.007914, whole genome shotgun sequence.
ACCESSION   DBMDUA010000029 DBMDUA010000000
VERSION     DBMDUA010000029.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 19814)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19814)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19814
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1673_length_19814_cov_3.007914"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            333..515
                     /locus_tag="ACZPCL_03975"
     CDS             333..515
                     /locus_tag="ACZPCL_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161681.1"
                     /translation="MAKNDYSNDYSNRYSNQMDDCSNMNQENKAKNKTENKTENTTRT
                     TSKNNMKNNSRNKTEY"
     gene            724..1035
                     /locus_tag="ACZPCL_03980"
     CDS             724..1035
                     /locus_tag="ACZPCL_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR1161682.1"
                     /translation="MTGFPMLPYWEFDRWIEIGRVRRIIDLREPWLYEEERIRGSENI
                     PYDEFWDHMGEINYGEMTVFLCERGAKSMVICRDLWRMGYEVADLAGGMVNYRGKYID
                     R"
     gene            1137..1691
                     /gene="yfcE"
                     /locus_tag="ACZPCL_03985"
     CDS             1137..1691
                     /gene="yfcE"
                     /locus_tag="ACZPCL_03985"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HIR1161683.1"
                     /translation="MKILFASDIHGSAYYCRRLLDIYKETKASRMVILGDILYHGPRN
                     DLPKEYAPKEVIAMLNPLKDQIYAVRGNCDTEVDQMVLQFPILADYALLVLDGKTFYA
                     THGHVFNQDHLPPMQDGDILVHGHTHLLKAEKVETECGTITVLNPGSVSIPKGGNPNT
                     YAILENGVFTIYSLDGEVVKELKL"
     gene            1778..2947
                     /locus_tag="ACZPCL_03990"
     CDS             1778..2947
                     /locus_tag="ACZPCL_03990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR1161684.1"
                     /translation="MKQTYELIAPCHFGLEAVLKKEILDLGYEISQVEDGRVTFIGDD
                     EAICRANVFLRTAERILLKAGSFRAETFEELFQGTKAIAWEEFIPEDGKFWVAKASSI
                     KSKLFSPSDIQSIMKKAMVERMKEHYHVSWFPEDGAKFPLRVFLYKDIVTIGIDTSGD
                     SLHKRGYRTLTSKAPITETLAAALIMLTPWNKDRILVDPFCGSGTFPIEAAMMAANMA
                     PGMNRSFLSEDWKNIIKRKYWYEAMDEANDLLDDTVKVDIQGYDIDGDIVKAARANAQ
                     SAGVDHMIHFQQRPVNALSHPKKYGFIISNPPYGERIEEKKNLPALYTEIGERFAALD
                     AWSMYLITSYEDAEKYIGRKADKNRKIYNGMLKTYFYQFMGPRPPKRSQENRNVD"
     gene            2961..4478
                     /locus_tag="ACZPCL_03995"
     CDS             2961..4478
                     /locus_tag="ACZPCL_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HIR1161685.1"
                     /translation="MQLKKAVSTFAGTALSGILCAVLLTGCGTLQNGEMPLPAESETS
                     RSNDQTEEKTKGEDAEDGEITGSTEKTDDTEEPKTSGTGKTVPEIWNREWPSNVDLPS
                     SYDYREHERAPKIGNQGSLGTCWAFASLTALESTLLPKQKETFSVDHMSMHNHFLLGQ
                     DEGGEYTMSMAYLLSWEGPVWESEDPYGDGFSPDGLKAREHVQEIQILPSKDYEAIKK
                     AVYLEGGVQSSLYTSMRDYESESVYYNRDTNSYCYIGTEKPNHDSVIVGWDDDYPKEN
                     FNMELEGDGAFICTNSWGENFGDQGYFYVSYYDTNIGVHNIVYTGIEPTDNYDNIYQS
                     DLCGWVGQIGYGEDTAWFANAYKTKTKEELAAAGFYATGAGTEYKIYAARNLPDRDST
                     GKEKLHQDHMMEMALNKRILLAEGKMEYTGYYTIPFNEKIVLDQGEKFAIIVEIKTPD
                     TVHPVAIEYDAGDGIAQVDLTDGEGYLSHNGSLWEHVEETQKCNLCLKVYTHTEK"
     gene            4509..5114
                     /gene="rdgB"
                     /locus_tag="ACZPCL_04000"
     CDS             4509..5114
                     /gene="rdgB"
                     /locus_tag="ACZPCL_04000"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR1161686.1"
                     /translation="MEKKIIFATGNAGKMREIRAILSDLGLPVLSMKEAGVDLDIVED
                     GKTFAENAKIKAMAVWKQTGGIVLADDSGLAVDYIGGEPGVYSARYMGEDTSYEIKNR
                     NIIERLSGAEGKERSARFVCNIAAVLEDGRVIQREAYMEGLIAKEPAGNGGFGYDPIL
                     YLPEFGKTSAELTMEEKNQISHRGKALEAMKKALKEEEAGE"
     gene            5111..5608
                     /locus_tag="ACZPCL_04005"
     CDS             5111..5608
                     /locus_tag="ACZPCL_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HIR1161687.1"
                     /translation="MKILIVSDTHRKDENLKWVIRKTKPFDMLIHLGDAEGSEYEIMK
                     WVDKDCDLEMIMGNNDFFSQLEREKEIMIGKYKVLLTHGHYYNVSTGPAYLKQEARER
                     GFDIVMFGHTHRPYFDIDKSGEKELITLNPGSLSYPRQEGHRPSYMLMKIDDNGEARF
                     EQHYL"
     gene            5797..5871
                     /locus_tag="ACZPCL_04010"
     tRNA            5797..5871
                     /locus_tag="ACZPCL_04010"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5832..5834,aa:Pro,seq:tgg)
     gene            5900..5970
                     /locus_tag="ACZPCL_04015"
     tRNA            5900..5970
                     /locus_tag="ACZPCL_04015"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5932..5934,aa:Gly,seq:tcc)
     regulatory      6043..6161
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6384..7025
                     /locus_tag="ACZPCL_04020"
     CDS             6384..7025
                     /locus_tag="ACZPCL_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032217 - riboflavin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0032218 - riboflavin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1161688.1"
                     /translation="MMERKLLNTKNVVLMGMFGALAAVLMLFEVPLPFLAPSFYGLDF
                     AEVPMLVGTFALGPAAGIVMQIVKILIKLILKPTSTGFVGEFANVVMSCALILPAGFI
                     YRFKKSKTGAVAGMAVGTVLMAVVGVVMNALVMIPFYSNFMPIDTIIKAGAAVNPAVS
                     SVWTLAIFCVGPFNLVKGALTSMITAVIYKRISVLIHGASGAYGVKKSRLKKA"
     gene            7140..7213
                     /locus_tag="ACZPCL_04025"
     tRNA            7140..7213
                     /locus_tag="ACZPCL_04025"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7174..7176,aa:Arg,seq:tct)
     gene            7246..7319
                     /locus_tag="ACZPCL_04030"
     tRNA            7246..7319
                     /locus_tag="ACZPCL_04030"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7280..7282,aa:His,seq:gtg)
     gene            7362..7434
                     /locus_tag="ACZPCL_04035"
     tRNA            7362..7434
                     /locus_tag="ACZPCL_04035"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7395..7397,aa:Gln,seq:ttg)
     gene            7524..7847
                     /locus_tag="ACZPCL_04040"
     CDS             7524..7847
                     /locus_tag="ACZPCL_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161689.1"
                     /translation="MEIFGFNFKSKAEKEKEARDYMERVFPGGEKEREQVRQELTKRL
                     PEKDEMDLLLCYVHIRDRMTGTGLSFEQVAGEVEKLQWIPDMDQKSLDAIREVMEQVC
                     AEHKK"
     gene            8020..8093
                     /locus_tag="ACZPCL_04045"
     tRNA            8020..8093
                     /locus_tag="ACZPCL_04045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8054..8056,aa:Arg,seq:acg)
     gene            8191..9912
                     /gene="recJ"
                     /locus_tag="ACZPCL_04050"
     CDS             8191..9912
                     /gene="recJ"
                     /locus_tag="ACZPCL_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR1161690.1"
                     /translation="MTKAVWMLHAKKADFNGLAARFHISPITARIIRNRDITDIDEFN
                     KYLNGSLKDLYEPRLLPDMEKAISILKEKIAAGVRIRIVGDYDIDGVCSTCILYKGLS
                     RVGASVDYVIPERIKDGYGINEHIIEKAAADGIHMILTCDNGIAAIDQIAEAKELGMT
                     VVITDHHDIQVDEVSETEIMPSADAIVNPKRKDSKYPFSEICGAMVAYKLIQVLFEEY
                     GIDRQEWLDMLELAAIATVGDVMKLKDENRIVVKEGLKKLANTKIQGLVSLMEKNTLD
                     PEHISAYHVGFVLGPCLNASGRLKTAQMAMELLLAENKAQADELADALKALNDERKDM
                     TKDGTDAAIEQVETELKDDKVLVVFLPDCHESLAGIVAGRIREYYNKPVFVLTRAEGC
                     AKGSGRSIEAYHMFHALVEVKDLLLKFGGHPMAAGFSLEEANIEEFRRRLNENAAKRL
                     TEDDFIPRVWIDVPMPFEYITESLIRELELLEPFGQGNEKPQFALKSLKIRSARVFGR
                     NRNVVKLSLMNERGFSMDGVVFTEGDLFMEEMGNSQSMDIIYYPTINEYNGNRSLQLV
                     VKDWKFH"
     gene            10122..11369
                     /gene="glyA"
                     /locus_tag="ACZPCL_04055"
     CDS             10122..11369
                     /gene="glyA"
                     /locus_tag="ACZPCL_04055"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR1161691.1"
                     /translation="MVNTVMDFITSYDKEVGEAIQAECGRQRRNLELIASENIVSEPV
                     MMAMGTVLTNKYAEGYPGKRYYGGCQYVDVLETIAIERAKKLFGCDYANVQPHSGAQA
                     NMAVFIAMLKPGDTVMGMNLDHGGHLTHGSPVNFSGLYFNIVPYGVDDEGFIDYDELE
                     RKAKEAKPKLIVAGASAYARTIDFKRFREIADEVGAYLMVDMAHIAGLVAAGEHPSPI
                     PYADVVTTTTHKTLRGPRGGLILANKEAAEKFNFNKAIFPGTQGGPLEHVIAAKAVCL
                     GEALKPEFKEYAHQVVLNAKALADALQKQGFKILTGGTDNHLMLLDLRGMDISGKELQ
                     NRCDEVFITLNKNTVPNDPRSPFVTSGVRIGTPAVTTRGLKEEDMPKIAECIWLAATD
                     FENKKEYIKAEVTKLCDKYPLYE"
     gene            complement(11475..15734)
                     /locus_tag="ACZPCL_04060"
     CDS             complement(11475..15734)
                     /locus_tag="ACZPCL_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008753632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HIR1161692.1"
                     /translation="MDQTTTYYTLGIDIGSTTVKVALLDQDLHIIFSDYERHYANIQE
                     TLAGLLKKALNQTGPIDIIPVITGSGGLTLSSHLHVPFVQEVVAVASALQDYAPQTDV
                     AIELGGEDAKIIYFTGGIDQRMNGICAGGTGSFIDQMASLLQTDAAGLNTYAKDYKAI
                     YPIAARCGVFAKSDIQPLINDGATREDLAASIFQAVVNQTISGLACGKPIRGNVAFLG
                     GPLHFLPELRKAFVRTLHLTPEQTIAPDHSHLFAAVGAAMNPKEGQEPVAITDMIDRL
                     SNGIKMDFEVKRMDPLFKDQAEYDAFIEDHGHNHVRSGNLSTYEGNCYLGIDAGSTTT
                     KVALVGEDGTLLYRFYDNNNGSPLATAIRAVTEIKEQLPETAHIAFSCSTGYGEALLK
                     AALMLEEGEVETISHYYAAAFFEPDVDCILDIGGQDMKCIKIKDGTVDSVQLNEACSS
                     GCGSFIETFAKSLNYSVKDFAKEALFAKNPTDLGTRCTVFMNSNVKQAQKEGATVADI
                     SAGLAYSVIKNALFKVIKITNASDLGKHVVVQGGTFYNDAVLRSFEKISGCRAVRPDI
                     AGIMGAFGAALIAKERFHMDGEKETTMLPLDKIIALQYTTSMTRCKGCNNHCVLTINQ
                     FGSGRHYISGNRCERGLGLAKAKKEIPNLFDYKYHRMFDYEPLPLDQADRGVVGIPRV
                     LNMYENFPFWAVFFKKLRYHVTLSPQSTRQIYELGIESIPSESECYPAKLVHGHISWL
                     IHQGVKFIFYPCIPYERNESPEAGNHYNCPMVTSYAENIKNNVEELETEHVDFMNPFM
                     AFTNEKILTDGLVREFVKKFQIPEAEIRMAAHAGWEELLASRSDMEKKGEETLAWMKE
                     HGKRGIVLAGRPYHVDPEIHHGIPELIASYGFAVLTEDSVSHMGKVERPLVVTDQWMY
                     HSRLYEAASFVKTRDDLDLIQLNSFGCGLDAVTTDQVAEILTKSGKIYTVLKIDEVNN
                     LGAARIRIRSLIAALRVRDQKHYERKVVSSAYNRVLFTKEMKKDYTLLCPQMSPIHFD
                     LIEPAIRAFGYNMEILQNHNRNAVDVGLQYVNNDACYPSLIVIGQIMDALLSGKYDLN
                     RTAVFMSQTGGGCRASNYIGFIRRALEKAGMPQVPVISVNANGMETNPGFSITLPMMT
                     KAMQAVVYGDIFMRVLYATRPYEAVPGSANALHEKWKKICIKALSKRQPAMMEFAKNV
                     RGIIRDFDNLERLDIKKPKVGIVGEILVKFSPLANNHVVELLESEGAETVMPDLLDFL
                     LYCFYNSNFKAEHLGMKKSTARLCNAGIALLEFFRKTARKELAASKHFTPPSEIRDLA
                     EMAKGFVSLGNQTGEGWFLTGEMLELIHSGVKNIICTQPFGCLPNHIVGKGVIKELRK
                     NYPESNIIAVDYDPGASEVNQLNRIKLMLATAQKNLKAENA"
     gene            16032..16973
                     /locus_tag="ACZPCL_04065"
     CDS             16032..16973
                     /locus_tag="ACZPCL_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIR1161693.1"
                     /translation="MIRIFKTEDGAMHEKEEMQPGCWIALTNPTASEILEIADACQID
                     PDHLKAPLDEEERSRIEAEDEYTLILVDIPSIEERNGKDWFVTIPMAIITTKDVLITV
                     CLEETPILTAFMDGRVRDFHTFMKTRFILQILYKNATQYLQYLRIIDKKSEVIENKLH
                     QSQKNEELIELLELQKSLVYFTTSLRSNEVVLEKLLRIDKIKKYPEDTDLLEDVIVEN
                     KQAMEMTSIYNGILSGTMDTFASVISNNLNIVMKFLATVTIVMSIPTMVASFYGMNVR
                     ASGMPFAEHPYGFGIVLFFTLVLTLIVAYIFNKKDLF"
     gene            17009..17881
                     /locus_tag="ACZPCL_04070"
     CDS             17009..17881
                     /locus_tag="ACZPCL_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161694.1"
                     /translation="MGKFSNNWKRRNGNFLGFRSIIDIVCALEILGVVLFLVAPQFVM
                     NYLILNPAAILHGQIWRIVTFLVYPPAITGSDAIMFVLMNALGIYCIRAFGTIVEQVW
                     GRFRFNCYIIGGVLLHSVVAIGIYLVTGLSLPLFPTYLVYSFFFVFALMFPDMQVLFM
                     MVLPLKASWIAVAEAGIYIYSFITGGLIEKIEIVLSLILIGVFFGWMTFAGPSGFKQK
                     KRSQEFQKTTQKMKVVKRGHKCAVCGRTDKDSPGMEFRYCSKCEGNYEYCMDHLYTHK
                     HVKKEDADNSNSND"
     gene            18010..19464
                     /gene="pyk"
                     /locus_tag="ACZPCL_04075"
     CDS             18010..19464
                     /gene="pyk"
                     /locus_tag="ACZPCL_04075"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR1161695.1"
                     /translation="MEEFENMKKTKIVCTMGPNTNDKNIMLELAKNGMDVARFNFSHG
                     DYAEHQSRLEILKEVRKELDRPVAALLDTKGPEIRTGVLKDGKKVSLKEGQTFTLTTR
                     EIVGDETITHINYSGLNEDVAAGNKILIDDGLIELEVVKVDGTEIVCTVINGGELGEK
                     KGVNVPNVKIKLPALTEKDKEDIRFGIRQGFDFIAASFVRTADCIREIKEMLDAEGSA
                     IKVIAKIENAEGIENLDEIIAVADGIMVARGDMGVEIPAQEVPHIQKEIIRKCNEACK
                     TVITATQMLDSMIRNPRPTRAEVTDVANAVYDGTDAVMLSGETAMGKYPIDALKMMVS
                     IVEETEAYLDYSAYRRRKVTEENMKNISNAVCSASVSTAHDIGADYIIAPSITGFTSM
                     MLSKWRPAARIIGMSPSTTTVRQMMLQWGVTPVWSRRAESTDELIENSLEELKSGNVI
                     NSGDLAVITAGIVTYARRHEAATATNIMRVVSVD"
BASE COUNT         5925 a         3919 c         4830 g         5140 t
ORIGIN      
        1 catgttgcct cacattagtt ataatataaa tacagggaaa atgcaagtaa attcccagga
       61 aaaagggggg aattacgtta cagttcagcc atgacatctt cttgtttcca tattcatgaa
      121 aacaagaagc ttggtggtcc caccatatgc tgatttggat gaaaaagtgc ttatgcaagc
      181 aggcttgcaa cacactttct cattcaaacc agcgcatggc tgaactgtaa aataaaattc
      241 tgcaaaagcc acgtataaac catcaggata cagccataat aaagctgtaa cttgaagtta
      301 atataaaaaa atatcttttt ggaggtattg aaatggcaaa gaatgactac agcaatgatt
      361 acagtaaccg ctacagcaac cagatggatg actgcagcaa catgaaccag gagaacaagg
      421 ctaaaaacaa gaccgaaaat aaaaccgaga acacaaccag gaccacatct aagaataaca
      481 tgaagaataa tagcagaaat aaaactgaat attaaatccc tggatctgta agtactggca
      541 agagtacaga taccagaaag aaaacggtca ttgaaaggaa cagactttca gtgaccgttt
      601 tttatgtact gctcccagat tgtacccacc agacaggacg ggcatatatt gataatagga
      661 agacttgtct ataacgtttt gaaatagttc gtatattcgc atatctggaa agaaggtgga
      721 aatatgactg gttttccaat gcttccttac tgggaatttg accggtggat agagatcggg
      781 cgggtacggc ggatcattga cttaagggaa ccatggctgt atgaagaaga acggatccgt
      841 ggaagtgaaa atattcccta tgatgagttc tgggatcata tgggggagat aaattatgga
      901 gaaatgaccg tattcttgtg tgaaagaggc gccaaaagca tggtcatctg ccgggacctg
      961 tggcgcatgg gatatgaggt ggcagacctt gcaggcggaa tggtgaatta cagaggaaaa
     1021 tacattgaca gatagtagtt tggggctgta agataatggg gcaggagaaa aacaagcgcc
     1081 gctgtaaggc ggcatttgtt tttcttgcac cattaacgaa tggaaggaga aatttcatga
     1141 aaattttatt cgcatccgat atccacggtt ctgcgtacta ttgccgcaga ctgttagaca
     1201 tttataaaga aacaaaagca tccagaatgg tcattttagg agacatcctt tatcatggcc
     1261 cgagaaatga tcttccaaag gagtatgcac caaaagaagt cattgccatg ttaaatccat
     1321 taaaggacca gatctacgct gttcgtggca actgtgatac agaagtagat cagatggtac
     1381 ttcagttccc gatcctggca gattatgcgc tgttagtatt agacggaaag acattttacg
     1441 caacccatgg acacgtattt aatcaggatc atctgccgcc tatgcaggat ggagatatcc
     1501 tggtacatgg acatacccat ctgctgaaag cagaaaaggt ggaaacagaa tgtggaacca
     1561 ttaccgtatt aaatccgggt tccgtttcta ttcctaaggg cggaaatcca aatacttatg
     1621 cgatcctgga aaatggtgta tttaccattt attccctgga cggcgaagta gtaaaagaac
     1681 tgaagctgta aatgctgaac ggtaacgtta catttataat gtgaaactga aaacgaaact
     1741 taaaatggaa tgaatttaga actgtgagga cgaaggattg aaacagacct atgaactgat
     1801 cgcaccctgt cattttgggc tggaagctgt attaaaaaaa gagatactgg acctggggta
     1861 tgagatctcc caggtagaag atggaagagt tacttttatt ggcgatgatg aagcaatctg
     1921 ccgtgccaat gtatttttac gcactgcaga gcgtatcctg ttaaaagcag gcagtttccg
     1981 tgcagagacc tttgaggaac tgtttcaggg aaccaaagct attgcctggg aagaatttat
     2041 cccggaagat ggaaaattct gggttgcaaa ggcttcttct attaaaagca agctgttcag
     2101 cccttccgat atccagtcca ttatgaaaaa ggccatggta gagcggatga aagagcatta
     2161 ccatgtatcc tggttcccag aggacggggc aaaattcccg ttgcgtgtgt tcttatataa
     2221 ggatattgtt accatcggca tagataccag tggtgactcc cttcataaaa gaggctaccg
     2281 tactcttacc agcaaggcgc ctattacaga aacactggca gcagccctga tcatgcttac
     2341 tccatggaac aaggaccgta ttctggtaga tccattctgc ggaagcggta ctttcccgat
     2401 tgaggcagcc atgatggcag ccaatatggc accaggtatg aaccgttcct tcttatctga
     2461 ggactggaag aatattatta agagaaaata ttggtatgaa gccatggacg aagcaaacga
     2521 cctgttagat gatacagtaa aagtagatat ccagggctat gatattgacg gggatattgt
     2581 gaaagcagcc agagccaatg cccagtctgc aggggtggac cacatgatcc atttccagca
     2641 gcgtccggta aacgctctca gccatccgaa gaaatatggt tttatcattt ccaacccgcc
     2701 ttatggcgag cgtattgaag agaaaaagaa cctcccggct ctgtatacag agatcggtga
     2761 gcgttttgca gcattggatg cctggtccat gtatctgatc acctcttatg aggatgcaga
     2821 gaaatacatt ggccgcaagg cagacaaaaa ccgcaagatc tacaatggta tgttaaaaac
     2881 ctatttctat cagttcatgg gtcccagacc gccaaaacgc agtcaggaga accgaaacgt
     2941 tgattaaaaa aagaataaaa atgcagttaa aaaaagccgt ttccacgttt gcgggaacgg
     3001 ctttgtctgg aatattgtgc gcagtccttc ttacaggctg cggtacgttg caaaatgggg
     3061 aaatgcctct tcctgcagaa agtgagactt ccagatccaa tgaccagacc gaagaaaaaa
     3121 caaaggggga agatgccgaa gacggggaaa taactggcag tacagaaaag acagatgata
     3181 cagaggagcc aaagacgtcc ggaactggga aaacagtacc tgagatctgg aaccgggaat
     3241 ggccttccaa tgtagatctg ccttcttctt atgactacag agagcatgaa agagcaccaa
     3301 aaatcggaaa tcagggctct ttaggcacct gctgggcctt tgcttccctt actgctttag
     3361 agtctaccct gctgcccaaa cagaaagaga cgttttcagt ggatcacatg tccatgcaca
     3421 atcattttct cctgggacag gatgaggggg gagaatacac catgtctatg gcgtatctgc
     3481 tctcctggga aggccctgta tgggagtcgg aagatcctta tggagatggt ttctcgccag
     3541 atggtttaaa agccagggaa catgtccagg agatacagat actgccgtca aaagattacg
     3601 aagcgatcaa aaaagcagtt tatctggaag gcggtgtgca aagttccctt tatacgtcca
     3661 tgcgggatta tgaaagcgag tctgtttatt acaatcgtga taccaattcc tattgttata
     3721 tcggtacgga aaagcccaac catgattctg tgatcgtagg ctgggatgat gattatccaa
     3781 aagaaaactt caatatggag ctggaagggg acggcgcttt tatctgtacc aacagctggg
     3841 gcgagaactt tggtgaccag ggctattttt acgtgtccta ttatgatacg aatatcgggg
     3901 tccataatat tgtatacacg ggaattgagc ctacagacaa ttatgacaac atttatcaaa
     3961 gtgatctgtg tggatgggta ggacagatcg gatatgggga agacacggcc tggtttgcca
     4021 atgcttacaa aacaaaaacc aaggaagaac tggcagcagc cggtttttat gcaacagggg
     4081 ctggcacaga gtataaaatc tatgcagcca gaaatctgcc ggacagggac agtacaggaa
     4141 aagaaaaact tcatcaggat catatgatgg aaatggcttt aaataagcgg atattgttgg
     4201 cagaaggaaa aatggaatat accggttatt atacgatccc ttttaatgaa aaaatagtat
     4261 tggaccaggg ggaaaaattt gctataatag tggaaataaa aacaccggat actgttcatc
     4321 cagtggccat tgagtatgat gcaggagatg gcattgccca ggtggacctg acagacggtg
     4381 aaggttattt aagccacaac ggttcgctct gggaacatgt agaagagacc cagaagtgca
     4441 atctgtgcct gaaagtatat actcatacag aaaaatagaa gctgatatcc atatgaaaag
     4501 gtgataacat ggaaaagaaa atcatatttg caactggaaa tgcaggaaaa atgcgggaga
     4561 tccgcgcaat cctttctgat ctgggacttc cggtgctttc tatgaaggaa gcaggagttg
     4621 atctggatat tgtggaggat ggaaaaacat ttgctgaaaa tgcaaagatc aaggcaatgg
     4681 cagtgtggaa gcagacaggc ggtatcgttt tagcagatga ttccgggctg gcagtagatt
     4741 atattggcgg tgaaccggga gtttattctg ccagatatat gggggaagac acctcttatg
     4801 agatcaaaaa ccgcaatatc attgaacgcc tctccggtgc agagggaaaa gaacgcagtg
     4861 cccgttttgt ctgtaatatt gccgctgtct tagaggatgg aagagtgatc cagcgagaag
     4921 cctatatgga aggtctgatc gcaaaggaac cggctggaaa tggtggtttc ggttatgatc
     4981 ctattctgta tcttccggaa tttggaaaaa catcagcgga acttaccatg gaagaaaaga
     5041 accagatcag tcacagagga aaagcactgg aagctatgaa aaaggcttta aaggaagagg
     5101 aagcaggaga atgaaaatat tgatcgtcag tgatacccac agaaaagatg aaaatttaaa
     5161 atgggtgatc cgtaaaacaa agccttttga tatgctgatc catttgggag atgcggaagg
     5221 cagtgaatat gagatcatga aatgggtaga taaggactgt gacctggaaa tgatcatggg
     5281 aaacaatgac tttttctccc agttagagag ggaaaaagag atcatgatcg gaaaatacaa
     5341 ggtacttctg acccatggac attattataa tgtatcaaca ggcccggctt atctgaaaca
     5401 ggaagcaaga gagcggggat ttgatattgt tatgttcggt catacccaca gaccttattt
     5461 tgacattgat aaatccggtg aaaaagagct tattacctta aatccgggca gcctctctta
     5521 tccaaggcag gaaggccaca gaccgtctta tatgctgatg aagatcgatg ataatgggga
     5581 agccagattt gagcagcatt atttatagtt acagttcagc caattcagca tacggtggat
     5641 ccgccaggct tcttgttttc atggatcatg gaaacaagaa gatgccaggg ctgaactgta
     5701 aacgatttgt gaaatacttg aaaaaactct taaaaaaaac ttataaaaaa gtgttgacaa
     5761 aattataaag gtcatatata ataatctacg ttgcgacggg gtgtggctca gcttggctag
     5821 agcgcctggt ttgggaccag gaggtcgcag gttcgaatcc tgtcaccccg acttatacaa
     5881 aagaatagat atatgatatg caggtgtagt tcaatggtag aacactagcc ttccaagcta
     5941 gatacgtggg ttcgattccc atcacctgct ttttgtgtgc gtacattttc ccgccaaacc
     6001 ctattgacgg cgaaaataga agatagtata ctgatactca gataagttct tcggggcagg
     6061 gcgaaattcc ctaccggcgg tatagtccgc gaaccatgtt atatggccga tccggtgaaa
     6121 ttccggaacc gacagtaaag tctggatgag agaagaagat gcgactgttt tggtcctgat
     6181 gtagttcatg aaaagaaata cataggagaa acagaaaata tcagtaaatc cccggatagc
     6241 ttgatcggct atccggtttt ttatgctttt ctttttgaac tgatatgaaa gcgcatattc
     6301 ctcagaagat gaacttacga aaacgggctt tttcagatat ctgaaagaac caaaaagtat
     6361 atttcataac agggaggaac gtaatgatgg aaagaaaatt actgaacacg aaaaatgtag
     6421 tgcttatggg catgtttggt gcgttagcag cagttcttat gctttttgaa gtgccgctgc
     6481 catttttggc accaagcttt tatggacttg attttgcaga agtgcctatg ctggttggaa
     6541 cctttgcttt aggaccggca gccggtattg tgatgcagat cgtaaagatc ctgatcaaac
     6601 tgatcttaaa gccaacttct acaggttttg taggtgaatt tgccaatgta gttatgagct
     6661 gtgccctgat ccttccggca ggctttatct accgctttaa aaagagcaag accggtgctg
     6721 ttgcaggaat ggcagtagga acggttctta tggctgttgt gggcgttgta atgaatgctc
     6781 tggtcatgat cccgttttat tccaacttta tgcctatcga taccattatc aaagcaggag
     6841 cagctgtcaa tccggcagtc agcagcgtat ggacactggc tattttctgt gtgggaccat
     6901 ttaacctggt aaaaggtgcc cttacttcta tgatcactgc agttatctat aagcggatca
     6961 gtgttctgat ccatggagct tccggcgctt atggagttaa aaagagccgt ttaaaaaagg
     7021 catgaaaaag gctctgaaaa ttaatttgaa aaaaattaaa aaaatttgaa aaaagtgctt
     7081 gcatttatga tagctctgtg ataatatata tctcgttggt gcggtaagcg ctgacgaatg
     7141 agtctgtagc tcagttggat agagcaacgg ccttctaagc cgtgggtcgg gggttcgaat
     7201 cccttcaggc tcattaggca aaagcctaat aaaaaaatat tgatggtggg tatggcgaag
     7261 ttggttatcg cgccagattg tggctctgga ggccgagggt tcgaatccct ctactcaccc
     7321 taatcctctt tctggagcag gaagaggatt ttttaatatc ttgggctgtc gccaagcggt
     7381 aaaggcacag gactttgact cctgcatacg agggttcgaa tcccgccagc ccagttgact
     7441 atagcagtcg gatttctgaa aaagcaggag tctggctact ttttgtttgc agagaatata
     7501 tatgtggaga gacaggggag aaaatggaaa tatttggatt taatttcaag agcaaagcag
     7561 aaaaggaaaa agaggcaagg gattatatgg aacgggtatt tcctggcggt gaaaaagagc
     7621 gggaacaggt tcggcaggaa ctgacaaaaa gattaccgga aaaagacgaa atggacttgt
     7681 tattatgtta tgtccatatc agggaccgga tgactgggac agggctttct tttgagcagg
     7741 ttgcaggcga agtagaaaag ctgcaatgga tcccggatat ggatcagaag tctcttgatg
     7801 cgatccggga agtgatggaa caggtctgtg cagaacataa aaagtagtaa aattctttta
     7861 aaggcagaaa aagaaaacgg caaaaatcca gtaaatatgc gggtttgaca ggcttgaaaa
     7921 aaatatttaa atttctttta aaaaagtgtt gacaaaaacc atatcattta ttataataat
     7981 ctacgttgct gaggaacaca gcaagcaaca aaaagaaatg cgtcagtagc tcagttggat
     8041 agagcatacg gctacggacc gtagtgtcgg gagttcgaat cttctctggc gcatgtgaaa
     8101 agcagattct tcgtgagtct gctttttttg ttgtcatata atcgcaaata cactgaaaaa
     8161 acgatcgatc aacagaggat ggagaaaaga atgacaaaag cagtctggat gcttcatgca
     8221 aaaaaagcag attttaatgg tctggcggca agatttcata tcagtcccat tactgcccgt
     8281 attatcagaa acagggatat tacagatata gatgaattta ataaatattt aaacggaagt
     8341 ttaaaggatc tgtatgagcc aaggctgttg ccggatatgg aaaaggccat ttctatatta
     8401 aaagaaaaga ttgctgccgg tgtcaggatc cggatcgtgg gagattatga tattgatggt
     8461 gtgtgttcca cctgcattct ttacaaaggc ctcagccgtg taggtgcaag cgttgattat
     8521 gtaataccgg agcgcattaa agatggttat ggtattaatg aacatattat tgaaaaggcg
     8581 gctgcagacg gcatccacat gatacttacc tgtgataatg gaattgctgc tattgaccag
     8641 atcgcagaag caaaagaact gggaatgacg gtagtgataa cggatcacca tgatattcag
     8701 gtagatgagg tttcggaaac ggagatcatg ccttctgcag acgccattgt caatcccaag
     8761 agaaaagaca gtaaatatcc gttcagcgag atctgcggcg ctatggtggc ttataagctg
     8821 atccaggtac tttttgagga atatgggatt gaccgccagg aatggctgga tatgctggag
     8881 ctggcggcga ttgctacagt aggcgatgta atgaagctta aggatgagaa ccgcattgta
     8941 gtaaaagagg gacttaaaaa gctggctaat acaaagatcc agggtcttgt aagcctgatg
     9001 gagaaaaata cactggatcc ggagcatata agcgcctatc acgttggctt tgtgctgggc
     9061 ccctgtctga atgccagcgg ccgcttaaaa acggcccaga tggctatgga actgcttctg
     9121 gcagaaaata aggcccaggc agatgaactg gcagatgcgt taaaggcatt aaatgacgag
     9181 cgcaaggaca tgaccaagga tgggacagat gctgccatag aacaggtgga gacagagctt
     9241 aaggatgata aggtgctggt agtctttctt ccggactgtc atgagtccct ggcaggcatt
     9301 gtggcgggca ggatccgcga gtattataat aagcctgtat ttgtccttac aagggcagaa
     9361 ggctgtgcaa aaggctcagg acgttctata gaggcatacc acatgtttca cgctctggta
     9421 gaggtaaaag accttctgtt aaagtttggc ggccatccta tggcagcagg attttcactg
     9481 gaagaggcaa atatcgaaga atttcgcagg cggttaaatg aaaatgctgc aaaacggctt
     9541 acagaggacg attttatccc aagagtatgg atcgatgttc ccatgccgtt tgaatatata
     9601 acagagagcc tgatccggga attagagctt ttagagcctt ttggacaggg aaatgaaaag
     9661 cctcagtttg ccttgaaatc cctgaaaata cgaagtgcca gagtgtttgg ccgtaaccgg
     9721 aatgtagtga aattatccct gatgaatgaa cggggctttt ccatggacgg cgtggtattt
     9781 acagaaggtg acttatttat ggaagaaatg ggaaacagcc agagcatgga tattatctat
     9841 taccctacaa taaatgaata taatggaaac cgcagtctgc agctggtagt gaaggactgg
     9901 aagttccact gaggagatac ctctggtacg tgagtgccag gggtgttttt gcaggaagaa
     9961 atatgagtct gtatattttt tgccaaaatc gtaaaatgta tgcaaatgaa tacttgaaat
    10021 ttacatgact atggggtata atcgcagaaa acaccatatc tggataaata aactgacaaa
    10081 atacgatggg tatgggcaaa tactgaaagg aaggtagaac aatggtaaat acagttatgg
    10141 actttatcac ttcctatgat aaggaagtag gagaagcaat tcaggcagag tgcggccgcc
    10201 agagaagaaa tctggagctg atcgcctctg agaacattgt ttcagaacct gtaatgatgg
    10261 ctatgggaac tgtactgacc aataagtacg cagaaggtta cccgggtaag cgttattacg
    10321 gcggctgcca gtatgtagac gtactggaaa ccatcgccat tgaacgtgcc aagaagctgt
    10381 ttggctgcga ttacgcaaac gtacagccac actccggtgc acaggcaaat atggcagtat
    10441 ttatcgctat gttaaaacca ggagatacgg ttatgggtat gaacctggat catggcggtc
    10501 atctgacaca cgggagtcct gtaaacttct caggactgta ttttaacatt gttccttatg
    10561 gagtagatga cgaaggcttt atcgattatg atgagctgga gagaaaagca aaagaggcaa
    10621 agccaaagct gatcgtggca ggtgccagcg cttatgcaag aaccattgat tttaagcgtt
    10681 tccgtgagat tgctgacgag gtaggtgctt atctgatggt agatatggcc cacattgcag
    10741 gtcttgtagc agctggagag catccaagcc caatcccata cgcagatgtt gttacaacta
    10801 ccacccataa gactttaaga ggacctagag gcggtctgat cttagcgaat aaggaagctg
    10861 cagaaaagtt caactttaat aaggctattt tcccaggaac ccagggcggc ccattagagc
    10921 atgtgatcgc agcaaaggca gtatgcctgg gcgaagcatt aaaacctgaa tttaaggaat
    10981 atgcacatca ggtagtatta aatgcaaaag cactggcaga cgcactgcag aagcagggct
    11041 ttaagatcct tacaggcggc actgacaacc atctgatgct gttagacctg cgtggtatgg
    11101 atatttccgg taaagagctg cagaaccgct gtgacgaggt atttattacc ttaaacaaga
    11161 acacagtacc aaacgatcca agaagcccat ttgtaacttc cggtgtccgc attggtacac
    11221 ctgctgttac caccagaggc ttaaaggaag aggatatgcc aaagattgca gagtgcatct
    11281 ggctggcagc tactgacttt gagaataaga aagagtatat taaggcagag gttactaagc
    11341 tttgcgataa gtatccgctg tacgagtaaa gaaatacata gaaataacga tttaagatga
    11401 aaaacgccgg aaaatgttgg ttttacaaca tacatttccc ggtgtttttt gttgaaatat
    11461 ttcaaatgcg tttattaagc attctcagcc ttcagatttt tctgcgccgt agccagcatc
    11521 agtttaatac ggttcaactg gtttacttca ctggcgcctg ggtcgtaatc tacggcaatg
    11581 atgttggact caggatagtt ctttcgcagt tccttgatca caccttttcc tacaatgtgg
    11641 tttggcaggc agccaaatgg ctgggtgcag ataatgtttt taacaccact gtggatcagc
    11701 tctaacatct cgccggtcaa aaaccagcct tcacccgtct ggttgcctaa ggatacaaag
    11761 ccttttgcca tttctgccag atcacggatc tcagaaggcg gtgtaaaatg tttgctggcg
    11821 gccagctctt ttcttgcagt ctttctgaag aactctaaca gggcgatacc tgcattacaa
    11881 agccttgcag tggatttctt cattcccagg tgttctgcct tgaaattgct gttgtagaag
    11941 cagtaaagaa ggaaatccaa caggtcaggc attacagttt ctgcaccttc tgactccaga
    12001 agctctacta catggttgtt agccagagga gagaatttaa caaggatctc acctacgata
    12061 ccaaccttcg gcttcttaat atcaagacgc tccagattat caaaatcacg gataatgccg
    12121 cggacattct tagcaaattc catcatagca ggctgtctct tagaaagagc cttaatacag
    12181 atctttttcc atttctcatg aagtgcattg gcacttccag gaactgcctc atatggtctg
    12241 gttgcataaa ggacacgcat gaaaatgtca ccgtatacaa ctgcctgcat ggccttggtc
    12301 atcattggca gtgtaatgga gaaacctggg ttagtttcca tgccatttgc attgacagaa
    12361 ataaccggaa cctgaggcat accggctttc tccagtgccc ggcggataaa tccgatatag
    12421 ttggaggcac ggcagccgcc gccggtctgg ctcataaaga cagccgtgcg gtttaagtcg
    12481 tatttgccgg ataacagtgc gtccatgatc tgaccgataa cgatcaggga tggatagcag
    12541 gcatcattat ttacgtactg aagacctaca tctacggcat tgcggttgtg gttctgcagg
    12601 atctccatgt tatagccgaa agcgcggatt gccggctcga tcaggtcaaa gtggatcggt
    12661 gacatctgcg ggcataacag agtgtaatcc ttcttcattt ccttggtaaa cagcacacgg
    12721 ttgtaagcgc tggaaaccac tttccgctca taatgcttct ggtcacggac acgaagagcc
    12781 gcgatcaggg aacggatacg gatacgggct gctcccaggt tatttacctc gtcgatcttt
    12841 aatacagtat agatctttcc ggactttgtt aagatctcag caacctggtc tgtggtaaca
    12901 gcgtccagac cgcagccaaa ggagtttaac tggatcagat ccaggtcatc tcttgttttt
    12961 acaaagcttg cggcctcata aagacgggaa tgatacatcc actggtcggt gacaactaaa
    13021 ggtcgttcca ctttgcccat atgggaaaca gagtcctctg taagtacggc aaagccgtag
    13081 gaagcgatca gttccgggat accgtggtgg atctccgggt ctacatggta aggtcttccg
    13141 gccagcacaa tgccccgttt accatgttct ttcatccagg caagtgtttc ctcgcctttt
    13201 ttctccatat cagaacggga tgccagcagt tcttcccagc cagcgtgggc tgccatacgg
    13261 atctctgctt caggtatctg gaattttttt acaaattccc gtaccaggcc gtctgttaag
    13321 atcttttcgt ttgtaaatgc catgaatggg ttcatgaaat ctacatgctc tgtctctaat
    13381 tcttctacgt tatttttaat attttccgca taggaagtga ccattggaca gttataatgg
    13441 tttcctgcct cagggctttc attgcgctca taagggatac atggatagaa gataaacttc
    13501 acaccctgat ggatgagcca tgaaatatgg ccatgtacca gctttgccgg ataacactca
    13561 gactcacttg gaatggactc aatgcccagt tcgtagatct gtctggtgga ctgtggggaa
    13621 agtgttacgt ggtagcgcag ctttttaaag aaaacagccc agaatgggaa gttttcatac
    13681 atatttaaaa ctcttgggat acccactacg ccgcggtctg cctggtctaa aggcagcggt
    13741 tcatagtcaa acatccggtg atatttgtaa tcaaacaggt ttggtatctc ttttttcgct
    13801 tttgcaagtc caagaccacg ctcacagcgg tttccggaaa tataatggcg tccgcttcca
    13861 aactggttga tggttaaaac acagtggttg ttgcagcctt tgcaccgggt catagaagtg
    13921 gtgtactgca gtgcgatgat cttatcaaga ggcagcatgg tggtctcttt ttcaccatcc
    13981 atatggaaac gctcttttgc gatcagtgct gcaccgaaag cgcccataat accggcaatg
    14041 tccggacgga cagcacgaca gcctgaaatc ttctcaaagc tgcgaaggac agcatcatta
    14101 tagaaggtac cgccctggac aactacatgt tttccaaggt cagaagcgtt tgtgatctta
    14161 attaccttaa ataaagcgtt tttaataaca gaataagcca gacctgcaga gatatcagcg
    14221 actgtagcgc cttccttctg ggcctgcttt acattggagt tcataaatac agtacagcgg
    14281 gtaccaaggt ctgtaggatt tttggcaaat aatgcttctt ttgcgaaatc ttttacactg
    14341 taatttaagc tttttgcgaa agtttcaatg aaagagccgc agccggaaga acatgcctcg
    14401 tttaactgta cgctgtctac ggttccgtct ttgatcttaa tgcatttcat atcctgaccg
    14461 ccgatgtcca ggatgcagtc tacatccggc tcaaagaaag cagcagcgta gtagtgggaa
    14521 atagtctcta cttctccctc ctctaacata agagcagcct ttaaaagggc ttcgccgtat
    14581 ccggtagagc aggagaaagc aatatgggca gtttctggaa gctgttcttt gatctctgtc
    14641 actgcgcgga tggcagtagc cagaggactt ccattattat tatcataaaa acgatataat
    14701 aatgtgccgt cttctccaac aagagccact ttggtggtag tggaaccggc gtcaataccc
    14761 agatagcagt tgccttcgta agtagaaaga ttgccggaac gcacgtggtt gtggccgtgg
    14821 tcttcgataa aggcgtcata ttctgcctga tccttaaaca gaggatccat acgctttact
    14881 tcaaaatcca ttttaatgcc attggaaagc cggtcgatca tgtcggtaat agctaccggc
    14941 tcctggcctt cctttgggtt catggcagcg cctacagctg caaacaggtg ggagtgatcc
    15001 ggtgcaatgg tctgctccgg agtcagatgc agcgtgcgaa caaaggcttt tctcagttca
    15061 ggaaggaaat gcaaaggtcc tcctaaaaag gcaacatttc cacggatggg cttaccacag
    15121 gcaagaccgc tgatggtctg gtttactact gcctggaaaa tagaggcagc cagatcctct
    15181 ctggtagcgc cgtcgttgat cagcggctgg atatccgatt tcgcgaaaac accgcagcgg
    15241 gctgcaattg ggtagatagc cttgtaatct tttgcatatg tattcagtcc agcagcatcg
    15301 gtctgcaaaa gggatgccat ctggtcaatg aaagacccgg ttcctcctgc gcagataccg
    15361 ttcatacgct ggtcgatgcc ccctgtaaag taaatgatct tggcatcttc gccgcctaac
    15421 tcaatggcta cgtcggtctg tggcgcatag tcctggagag cagatgcaac tgctacaact
    15481 tcctggacaa aaggaacgtg taaatgagat gaaagtgtaa ggccgccaga gccggtgatc
    15541 accgggataa tatcaatggg gccggtctgg tttaaggctt tctttaaaag gcctgccaga
    15601 gtttcctgaa tattggcata atgacgctca taatcggaga atataatatg aagatcctga
    15661 tccagtaatg caactttgac agttgttgaa ccgatatcga tccccagggt gtaataagtt
    15721 gtagtctgat ccatagtata tggggatagc cccttacctc cttcgtgatt tgatgtactt
    15781 tcaagactct gagagtacat cctatagctt atgaaacgta attgtttgtt agaacatcat
    15841 gtctcttact gcaattccgg gcgaaaagcc caaaaaagca cattacagtc taacacaata
    15901 ccttttactt ggcaattgtt aaagggggtt aagaatttgt gaagaattga aaaaatatga
    15961 aacgatttga caaaaaccgg gccagtcact ataatgagta gggtgaaaat tcaaaatggg
    16021 ggagatttcg catgatacgg attttcaaga cagaagacgg tgcaatgcac gaaaaagagg
    16081 agatgcagcc aggctgctgg atcgccctga ccaatccaac tgcatcagag atcttagaga
    16141 ttgctgatgc ctgccagatc gatccggacc acctgaaagc acctctggat gaggaagagc
    16201 gctcacgtat tgaagcagaa gatgaatata cgctgatatt ggtggatatt ccttctatag
    16261 aagaaagaaa cgggaaagac tggtttgtga ccattcctat ggcgatcatt accaccaagg
    16321 atgtgctgat aaccgtctgc ctggaggaga ccccgatcct tactgccttt atggacggaa
    16381 gagtcagaga tttccacaca tttatgaaaa caaggtttat cttacagatc ctttataaaa
    16441 atgcaaccca gtatctgcag tacctgcgca ttatcgataa aaagagtgaa gtcattgaaa
    16501 acaaacttca ccagtcccag aaaaatgagg aactgatcga actgttagaa cttcagaaat
    16561 cactggtgta ctttaccacc tcccttcgtt ccaacgaagt ggtgctggag aagttattaa
    16621 ggattgacaa gatcaagaag tacccggaag atactgatct tctggaagat gtaatcgtag
    16681 agaataagca ggctatggag atgaccagca tttacaatgg cattttaagt ggtaccatgg
    16741 atacctttgc atctgttatt tcaaacaact taaacattgt tatgaaattc ctggcaaccg
    16801 ttaccattgt tatgtccatt ccaactatgg tggcaagttt ttacggtatg aacgtaagag
    16861 ccagcggaat gccttttgca gagcatcctt atggatttgg catcgtgctt ttctttacac
    16921 tggtattgac actgatcgtt gcttatatat ttaataagaa agatctgttt taaaatcagt
    16981 taaatcagtt taaaaaagga gtttttgaat gggaaaattt tccaataact ggaaacgccg
    17041 gaacggaaat ttcctggggt tcagaagtat cattgatatt gtatgcgccc tggagatcct
    17101 tggagtggtc cttttcctgg tagcaccgca gtttgtgatg aattatctga tcttaaaccc
    17161 ggcggctatc cttcacggtc agatctggcg tattgttaca tttttagtgt atccaccggc
    17221 cattacaggt tctgatgcca ttatgtttgt gctgatgaac gcccttggca tttactgtat
    17281 ccgtgctttt ggtaccattg tggagcaggt atggggaaga tttcgcttta actgctacat
    17341 tatcggaggt gtgctccttc attcggttgt tgccattggc atttacctgg taacagggct
    17401 ttcactgcca ctgttcccaa cttatttggt ttattccttc ttctttgtat ttgccctgat
    17461 gtttccggat atgcaggtac tgtttatgat ggtactgcca ttaaaggcaa gctggatcgc
    17521 agtggcagag gcaggaattt atatttattc ctttattaca ggcggactga tcgagaagat
    17581 cgagatcgtt ttaagtctga tccttattgg cgtattcttt ggctggatga cctttgcagg
    17641 tccttccggt tttaagcaga agaaacgctc ccaggaattc cagaagacca cccagaaaat
    17701 gaaagtggta aagagaggac acaagtgtgc agtctgcgga cgcacagata aagacagtcc
    17761 tggtatggaa ttccgttatt gttccaaatg tgaaggcaat tatgaatatt gcatggatca
    17821 tttgtacaca cataagcatg tcaaaaaaga ggatgctgat aacagtaaca gtaatgatta
    17881 atgcgaccgg catgctctgc gcttttgcac tgcatatccc cacttggcct gacacattta
    17941 ttcctgtgcc aagggcataa aaaggcagta aaagagcgaa tatactacta accccaattc
    18001 attttattta tggaggaatt tgaaaacatg aagaaaacta agatcgtctg cactatgggc
    18061 cccaacacaa atgacaagaa cattatgctg gaactggcta aaaatggaat ggacgtagct
    18121 cgttttaact tctcccacgg tgattatgca gagcatcaga gccgcctgga gattttaaaa
    18181 gaggttagaa aagaactgga ccgtccggta gcagctcttc ttgatacaaa gggaccggag
    18241 atccgtaccg gcgtattaaa agacggaaag aaagtttcct taaaggaagg acagaccttt
    18301 actttaacta ccagagagat cgttggtgat gagactatta cccatatcaa ctactctggc
    18361 ctgaatgaag atgttgcagc tggaaataag atcctcatcg atgacggcct gatcgagctg
    18421 gaagtagtaa aggtagacgg cactgagatc gtttgtaccg ttatcaacgg cggtgagtta
    18481 ggcgagaaga agggtgtaaa tgttcctaac gtaaagatca aacttccggc actgactgaa
    18541 aaagataaag aagatatccg ttttggtatc agacagggct ttgattttat cgcagcttct
    18601 tttgtacgta cagctgactg tatccgtgag atcaaggaaa tgctggatgc agaaggttct
    18661 gcaatcaagg taatcgctaa gatcgagaat gcagaaggta ttgaaaacct ggatgagatc
    18721 attgcagtag cagacggtat catggtagca agaggtgaca tgggtgttga gattccggct
    18781 caggaagttc ctcatatcca gaaagagatc atccgtaagt gtaatgaggc ttgtaagact
    18841 gttatcactg caacccagat gttagactcc atgatccgca acccaagacc taccagagct
    18901 gaggttactg acgttgccaa cgctgtttat gacggtactg acgcagtaat gctttccggt
    18961 gagactgcta tgggtaaata tccgattgac gcattaaaga tgatggtatc tatcgttgaa
    19021 gagacggaag cttacttaga ctacagcgca taccgccgtc gtaaagtaac tgaggagaat
    19081 atgaagaata tctccaatgc agtttgctcc gcttctgttt ctaccgcaca tgacatcggt
    19141 gctgattaca tcattgctcc aagtattaca ggatttacat ccatgatgct ttccaagtgg
    19201 agaccggcag cacgtatcat cggtatgtcc ccaagcacta ctactgtccg tcagatgatg
    19261 cttcagtggg gtgtaacacc tgtatggtcc cgtcgtgcag agagtacgga tgagctgatc
    19321 gaaaactctt tagaagagtt aaagagcggc aatgtgatca attctggtga tctggccgtt
    19381 attaccgcag gtatcgttac ctatgcaaga cgccatgagg cagcaacagc taccaatatc
    19441 atgcgtgtgg taagtgtgga ctagctgaat gctaccagat tgctttgcaa tcttcaaaac
    19501 attcattaaa aataatacta gacaaagaag tctgtctggg agtacaatta tccgcaaagg
    19561 aaatggtact cccttacagg cttcttttct tttacggagg ttatcctgta aaattaaaag
    19621 gaactgatat atatgcatag ataatgcata aaaataccat tgcgtaaagc aaagatcccc
    19681 cacagggaaa ggagataaga ttatggaaaa gaaaccattt gttacactgg cgcaggcaga
    19741 agagatcgcc aagacctatt caaccccatt ttatctgtat gatgaaaaag gtatcctgtg
    19801 caagctgaag catc
//
LOCUS       DBMDUA010000030        19757 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1675_length_19757_cov_2.959554, whole genome shotgun sequence.
ACCESSION   DBMDUA010000030 DBMDUA010000000
VERSION     DBMDUA010000030.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 19757)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19757)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19757
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1675_length_19757_cov_2.959554"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="ACZPCL_04080"
     CDS             complement(<1..1333)
                     /locus_tag="ACZPCL_04080"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purple acid phosphatase family protein"
                     /protein_id="HIR1161696.1"
                     /translation="MKKRRIAAGLLAGVLVIGGQQVWAATTHYNDSSVTGGSEQWQKW
                     VADWNQTATDFTKVSLTPGGAASELNFAWYSEGNEPTPVVYFGTDKDDLKACSGTASS
                     VDPSLTGGKPYSYNYVTVNGLKENTTYYYSVEKSGVRTPAEVYKTGSFENVKILYVGD
                     PQVGASKGQPQGEGKLSADSGAANTAARNDGFAWDRTLDAALAQNPDLNFVISAGDQV
                     NKTGKPKEEEYAAYLSADALKSLPVATTIGNHDSLNKDYSYHFNNPNPTDLGMTEAGG
                     DYYYSYGPGLFIVLNTNNYNVAEHEQAIQKAVANFPDTKWRIVTIHQDIYGSGLDHSD
                     TDGMILRTQLTPVFDKYDIDVVLQGHDHTYSRSKILYGDGKSHGSYEFRLDETGLDYD
                     WDHAYNNNTGESITLNPLSFDTAAVEAKDAFTADNKCYTIESSGKSTPPRLV"
     gene            complement(1546..3030)
                     /locus_tag="ACZPCL_04085"
     CDS             complement(1546..3030)
                     /locus_tag="ACZPCL_04085"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HIR1161697.1"
                     /translation="MPCTTILVGKKASYDGSTMIARNDDAGGNHFTAKKYVIVHPEEQ
                     PKVYKSVISHVEIPLPEHPMRYSAAPNAIPGQGIWAACGVNEKNVAMTATETITSNPR
                     VLGADPMVVYQPAEDGKAEVPGGIGEEDIVVLVLPYINSAREGVKRLGSLLEQYGTYE
                     KNGIAFQDANEIWWLETIGGHHWIARRVPDDSYVVMPNQLGLDVFDLEDALFEQKEYM
                     CSADMREFIEENHLDLSFDDCFNPRDAFGSHEDSDHVYNTPRAWFGLRYFNPHTMKWE
                     GEDADYTPESDDLPWCMVPEKKITVEDVKYVLSSHFQGTPYDPYAKHSDPVYRNKYRA
                     IGINRTDLLSLIQIRPYMPEEFQVIQWVAFASNPFNVLVPFYPQVSKTPEYMANTTAE
                     VSTENFYWASRMIAAMADAAYAKTSIYIERYRLAVGSKAHALIIKGDKAQQAAGTPEE
                     KEKIREEINEEMAAMLKKETGNTLNKVLFELSSSMKNAFSRSDA"
     gene            complement(3009..3407)
                     /locus_tag="ACZPCL_04090"
     CDS             complement(3009..3407)
                     /locus_tag="ACZPCL_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013703.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1161698.1"
                     /translation="MSAGLLLRFALPLFIGNLLQQFYNLADTSIAGHMLGDQALAEIG
                     ATAALYSLITNFAFGMNNGLALTVSRNFGAGNEKKMKQAVCWMTILSIGTALVLSGGF
                     LLFQKILLELLKIPEENMTGGMTLCHVQLY"
     gene            complement(3686..4795)
                     /locus_tag="ACZPCL_04095"
     CDS             complement(3686..4795)
                     /locus_tag="ACZPCL_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1161699.1"
                     /translation="MMAEERSSSYICAEDISVGYHNLPLIQKINLHVNRGEILTLIGP
                     NGAGKSTILKSLIGQLKLVKGAVWLDGTSMGTMKEKEIALRMSVMMTGRVKTERMTCE
                     EVVSMGRYPYTGKMGILTEKDWQIVRESLELVQGLELAEKDFEAVSDGQRQRVLLARA
                     ICQEPQVIVLDEPTAFLDIRHKLELLYLLKNMVREKKVAVLMSLHELDLAQKISDHII
                     CVKGDNSIGRFGTPEEIFSGDYIKELFQIEKGTYLTDYGSVELEAVKGEPKVFVIGGN
                     GTGIPVYRQLQKEGIPFAAGILWENDREYPVAEALASVVIREKAFESVSDATFETAVK
                     VLKNCEKVICCMENFGTYQEPCRQLWKIAKDLGKI"
     gene            complement(4800..5822)
                     /locus_tag="ACZPCL_04100"
     CDS             complement(4800..5822)
                     /locus_tag="ACZPCL_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR1161700.1"
                     /translation="MTGTAKKRIHIAGGFLLLVVALVLLFFWNIYSGSVSVEPKDVLR
                     LLFQGPDESTQARILWDIRFPRVLAAMLLGGALSVSGFLLQTFFANPIAGPFVLGISS
                     GAKLMTALVMIFLLGRGLVAGSVVLILASFIGAMLSMGFVILIANKVNNMSILVICGI
                     MVSYICSAVTDLVVTFADDSNIVNLHNWSLGSFSGITWENISVMAVVIFFCMICAMLM
                     AKPAGAYQLGEIYAASMGVNIKAFRLALILVSSILSACVTAFAGPVSFVGIAVPQLVK
                     SLFKTTKPIIMIPACFLGGAVFCSFCDRLARTLFSPVELSISTVTAVFGAPVVIYMMI
                     QTRGRR"
     gene            complement(5855..7111)
                     /locus_tag="ACZPCL_04105"
     CDS             complement(5855..7111)
                     /locus_tag="ACZPCL_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1161701.1"
                     /translation="MKKQIKKKLLKGFLLGTLTVCVLGMCAGCGQKTENIENTVTSPT
                     AQEQTGKTGQKSLSWSELTQTGSMDLSYADQFSVTYYGEENYALVIIGEDEKFLVVPE
                     GEPVPEDLPEDITPLLQPLTNMYLAATSAMDFFCHLDAVDQITLSGTDRSGWYLEEPK
                     KALEEGTMEYAGKYSAPDYERIVDKSCSLAIESTMIYHCPQVKEQLENLGVPVLVERS
                     SYEADPLGRMEWIKLYGVLTGKEQLAEELFEKEIKELENVSVQADEGQEHSDQTNQGK
                     TVAFFYITSKGSANVRKPGDYVAKMITMAGGTYVPQSAEEEEENALSTMNMEMEAFYA
                     KAKDADYIIYNSTIDGELTTMDEFLAKSPLLADFKAVKEGHVWCTGKNLFQETMGLGN
                     MIQDIHRMLTEETPDEGQMHYIHQLK"
     gene            complement(7142..8071)
                     /locus_tag="ACZPCL_04110"
     CDS             complement(7142..8071)
                     /locus_tag="ACZPCL_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161702.1"
                     /translation="MKIRKYILCAGIVGLVGMSSGCGQKTDSGATSQAVSEQADTKEN
                     AETSGAAGAETEKETAPTVQVVTDDMVPVEADALKDGVYPVEVDSSSSMFHITEAELT
                     VADGKMSAKLTLSGSGYGKVFMGTGEEAEKAGEGDYIQAEESADGKNVFEIPVEALDK
                     GIDCAAYSKKKETWYDRTLVVRASSLPMEAYKEGKMKTVESLGLEDGTYTVEVKLEGG
                     SGRTTVETPTQLKIQDGKATAVITWGSSHYDYMKVGEEKYEPVNTEGNSSFEIPVTVF
                     DQKQKVIADTTAMSVPHEIDYTLIFDSESIQPQ"
     gene            complement(8216..9256)
                     /locus_tag="ACZPCL_04115"
     CDS             complement(8216..9256)
                     /locus_tag="ACZPCL_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HIR1161703.1"
                     /translation="MKLKKAMLLFAAAAVAAGTVAGCSGSAKETTAASVAETNAEETK
                     EETTAEETQKEKTAAEEEDEENYDTGDAALDNTRNQDEIGEKELLVVSFGTSYNDSRR
                     LTIGAIEDAIEKAEPDFSVRRGFTSQIIIDHVKKRDDVTIDNVTEALDRAVNNGVKTL
                     VVQPTHLMNGLEYTDLVNEIAENADSFEKVVVGEPLLTSDDDFKAVIQAITDATKEYD
                     DGETAICFMGHGTEADSNQVYAKMQDMLTEEGFEHYYVGTVEATPSLEDVVAKVKEGS
                     YKKVVLEPLMIVAGDHANNDMAGDEEGSWKTTFEEAGYEVTCLVRGLGELEPIQQLFV
                     EHAKAAVDSLAK"
     regulatory      complement(9448..9686)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(9791..10732)
                     /locus_tag="ACZPCL_04120"
     CDS             complement(9791..10732)
                     /locus_tag="ACZPCL_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HIR1161704.1"
                     /translation="MKELLFMKPVFKEAVWGGRKLRDDFGYEIPSDSTGECWGIGAHK
                     NGDCEIAEGTYKGEHLSKLWEEHPELFGNEDGKYGKEFPLLIKIIDAKSDLSIQVHPD
                     NAYAKEHENGSLGKTECWYILDSEPGTQIVIGHHAKDKEELMQMVEEKRWKDLIREVP
                     VKKGDFYQIDPGCVHAIKGGTVILETQQSSDITYRVYDYDRKWNGKLRELHVKQSLDV
                     IKTPFQPAKDQRSITHTEGADLEHLETCPYYTVEKYDIHGTWDHVFEAGFVNASVIEG
                     EGSINGRKIKKGDHFIIPADFGTCSFEGNLSLICSWV"
     gene            10943..11836
                     /locus_tag="ACZPCL_04125"
     CDS             10943..11836
                     /locus_tag="ACZPCL_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1161705.1"
                     /translation="MELLQLRYFLVAAQYQHMTKAAEHLQIAQPALSQAIHRLEAELG
                     VPLFERKNRSIELNDEGKFLQKRLIPILSSLDRLPEELRKGKEAATHTVHLRLLSASA
                     LITNRIIAYRSLRPDVNFQLYQIPGQEDDFDVCVTARRADTAPKENDDATVMLEEDLY
                     LAVPSHSPYGSRESIRLTDTKDADYICLGGSKPIRQITNSYFMEAGFTPHIIFESDTT
                     ESVRNLIAAGMGIGFWPACSWGPLTTNFGGSPGHFPVKLLPITDQKCRRQIILTRSEK
                     GKKSPIVADFCDFLVEHNNWL"
     gene            complement(11909..12337)
                     /gene="atpC"
                     /locus_tag="ACZPCL_04130"
     CDS             complement(11909..12337)
                     /gene="atpC"
                     /locus_tag="ACZPCL_04130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR1161706.1"
                     /translation="MAAFWLKVIASDHVFYNGNCESLVVPAHDGEVGILPHREAMILA
                     IQEGELRFRVPGENQYREAVVGIGIVQVANNRVTVVVDTAERPEDIDEVRARQALERA
                     QEQLRQKQSIREYYMSKASMARALSRLKASQHKGSEYHGL"
     gene            complement(12337..13737)
                     /gene="atpD"
                     /locus_tag="ACZPCL_04135"
     CDS             complement(12337..13737)
                     /gene="atpD"
                     /locus_tag="ACZPCL_04135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR1161707.1"
                     /translation="MNKGKIIQVMGPVVDVAFENGNLPHIQDALTVMNNGKRCVMEVA
                     QHIGGDVVRCIMLASSEGLYKDMEVEATGQGIAVPVGEKTLGRLFNVLGDTLDGGESL
                     EGEEKWVIHRDPPSFADQSPVVEMLETGIKVIDLLAPYAKGGKIGLFGGAGVGKTVLI
                     QELITNIATEHGGYSIFTGVGERSREGNDLWTEMSESGVLKKTALVFGQMNESPGARM
                     RVAETGLTMAEYFRDVKHQNVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLATD
                     LGGLQERIASTKNGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRKIVEQGIYP
                     AVDPLESSSRILEEDVVGKEHYEVANKVKEMLQKYKELQDIISILGMEELSEEDKLTV
                     NRARKIQRFLSQPFHVAENFTGVPGKYVPVKETVRGFKAIIDGEMDEYPEAAFFNVGT
                     IDEVVEKAKAMQEGAS"
     gene            complement(13748..14641)
                     /gene="atpG"
                     /locus_tag="ACZPCL_04140"
     CDS             complement(13748..14641)
                     /gene="atpG"
                     /locus_tag="ACZPCL_04140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR1161708.1"
                     /translation="MANAREIQNRIKSIQDTRKITNAMYLISSTKLKKSKKLLEDTEP
                     YFYTLQSLIRRILRHMGDTEHPYFNARKEIKEEDRVRGLVVITGDKGLAGAYNHNVLK
                     LAHEWMEKSDKNRLYVVGELGRQYFAARHVPVEEQFHYTVQNPTMHRARLIASSLIED
                     YLEGRLDEVWIIYTQMINSMKVEAQMEQLLPLKQEDFSNVVIPADIHQEEIQMVPSPT
                     AVMDHVVPNYVTGFIFGALVESFCSEQNARMMAMQSASDNAAELLNELSIEYNRVRQA
                     AITQEITEVVGGARAQRKKRR"
     gene            complement(14608..16146)
                     /gene="atpA"
                     /locus_tag="ACZPCL_04145"
     CDS             complement(14608..16146)
                     /gene="atpA"
                     /locus_tag="ACZPCL_04145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR1161709.1"
                     /translation="MSTISSQEIISIIKGEIEDYDAHAGQVNETGSVIWVGDGIAIVY
                     GIDHAMYGEIVIFENGVKGMVQDIRKNEIGCILFGKDTGITEGTKVTRTKKKAGVPVG
                     AAFLGRVVNALGETIDGMGPAKEEDYLPVEQEAPGIAERKSVGVPMETGILAIDSMFP
                     IGRGQRELIIGDRQTGKTSIAMDTILNQKGKDVICIYVAIGQKASTIAKLVNTLKKND
                     AMDYTIVMASTASDPAPLQYIAPYAGTAMAEFFMRQGKDVLIVYDDLSKHAVAYRALS
                     LLLERSPGREAYPGDVFYLHSRLLERSSRLSDKLGGGSITALPIIETQAGDVSAYIPT
                     NVISITDGQIFLESDLFFSGMRPAVNVGLSVSRVGGAAQTKAMKKASGTIRIDLAQYR
                     EMEVFTQFSSDLDEATKAQLAYGKRLMELLKQPLGRPLSMAEQVITLCTATHKVMVSV
                     DVKQIKKFQMDMLAWFEDKHPEIGNEITEKKVLSDELAERIVEAAKEFKEAVWQTQER
                     SKTE"
     gene            complement(16158..16673)
                     /gene="atpH"
                     /locus_tag="ACZPCL_04150"
     CDS             complement(16158..16673)
                     /gene="atpH"
                     /locus_tag="ACZPCL_04150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR1161710.1"
                     /translation="MTEQARLYGTVLYELKVPEKMVRETGRIFQENPELKAVLDDPTL
                     QETKKEAIIGKIWKEPDFSSTMVHFLKKACEEGCIGQMEDMIKVWEKCLLDGAGILSA
                     SLFYVTRPDEEQLAGILEFLCREYKKKDVQLEMAAAPELMGGFVLKTGDVEYDYSLKG
                     QLERLFQTAGR"
     gene            complement(16666..17157)
                     /gene="atpF"
                     /locus_tag="ACZPCL_04155"
     CDS             complement(16666..17157)
                     /gene="atpF"
                     /locus_tag="ACZPCL_04155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR1161711.1"
                     /translation="MLNINFWNIAFTIINIVVLYLFLKHFLMKPLMSILEERKQMVEK
                     ELDKAAAANRDAGLLKKKYEVSLEKADEEAGRIVAQAKERAGEEYEKILSKADADASK
                     KLEDAKKMIDLEREKALNDLRASVTGLAMTATAKLLSEQAGPESDKKRYQAFLASAGE
                     KHD"
     gene            complement(17171..17395)
                     /gene="atpE"
                     /locus_tag="ACZPCL_04160"
     CDS             complement(17171..17395)
                     /gene="atpE"
                     /locus_tag="ACZPCL_04160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR1161712.1"
                     /translation="MSGLIALGAAVAVITGIGAGLGIGMATSKAVDAIARQPEADSKI
                     TKALLLGCALAEATAIYGFVIGLLIILFLK"
     gene            complement(17432..18118)
                     /locus_tag="ACZPCL_04165"
     CDS             complement(17432..18118)
                     /locus_tag="ACZPCL_04165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR1161713.1"
                     /translation="MDQLVDSLMEELNCNTVFTIPLFGGIDVLESVVVTWIIMAIIMI
                     GSLIWVHGLKIRKISGKQAALESGFSFLYNFFLDLLGEEGKDYIPYLITVVIYIAIAN
                     MIGLLGFKSPTKDLNVTASLAVMSIVLVEAAGIRKKGVKGWVKSFAEPIPIIAPINVL
                     ELFIRPLSLCMRLFGNVLGAVVVMALIKYLLPLVVPLPFSGYFDIFDGVIQAYVFVFL
                     TSLYIKEAIE"
     gene            complement(18776..>19757)
                     /locus_tag="ACZPCL_04170"
     CDS             complement(18776..>19757)
                     /locus_tag="ACZPCL_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR1161714.1"
                     /translation="FAGISGSSQADTAGVGKIFIPAMEKQGYDKGTSVGVTAASSTLG
                     SIIPPSITMVVYAGIANVSTGALFMSGLVPGILLGLAMMAVVKYYSKRKNFPKCDRVP
                     FKEVCRRTFQSLPALLTPIILIGGIVSGLFTPTESAAFACIYALLVGIFFYKTIKVKN
                     LPRILIETMKLSSLSLFALATANALGELLAYYQLNVIVQNFFANMPGGRLVFLLVVVA
                     FFMFVGTFMDAVPAMILFVPIILPAATSLQISPIILGLIIVVTLALGLVTPPYGLCLL
                     IASSISGITIEDAFKGTLPYFLSSIAVLLLLVIFPNFWLAIPATLFPGLF"
BASE COUNT         4998 a         5068 c         3829 g         5862 t
ORIGIN      
        1 atactaatct gggtggagtg gatttgccgg agctttcaat ggtatagcac ttattgtctg
       61 ctgtaaaagc atcttttgct tctacagcag ctgtatcaaa tgaaagagga tttaaagtaa
      121 tactctcccc tgtattgttg ttgtaagcat gatcccagtc atagtccaga cctgtttcat
      181 ccagtctgaa ttcataagag ccatgtgact ttccatctcc atagaggatt ttggaacggc
      241 tgtaggtatg gtcatgtccc tgaaggacta cgtcaatatc atacttgtca aatacaggtg
      301 tcaactgggt acgaaggatc ataccgtctg tatcagagtg atccaggccg gagccgtaga
      361 tatcctgatg gatggtcacg atacgccatt tggtatccgg gaagtttgca actgctttct
      421 gaatggcctg ctcatgttct gctacattgt agttgtttgt atttaataca atgaaaaggc
      481 caggtccata gctgtagtaa taatctcctc ctgcttctgt cattcccaga tcagtcggat
      541 ttggattgtt aaaatgatag ctgtaatctt tgtttaaaga gtcgtggttt ccaatggtag
      601 ttgctaccgg aaggctcttt aaagcatctg cggaaagata agctgcatac tcttcttcct
      661 ttggctttcc tgttttattt acctggtcac cggcagagat cacaaagttc agatccgggt
      721 tctgtgctaa agctgcatcc agtgtacggt cccatgcaaa accgtcattg cgggctgctg
      781 tattggcagc gccggagtca gcacttaatt ttccttctcc ctgaggctgt cccttagaag
      841 cacctacctg cgggtcgcct acataaagga tctttacatt ttcaaaactt cctgttttat
      901 aaacttccgc aggagttctc acaccgctct tttctacaga ataataataa gtagtgtttt
      961 ctttcagacc gtttacagtt acatagttgt agctgtaagg ttttccacct gtaagagatg
     1021 gatctacact gcttgcagtt cctgaacagg ctttaagatc atccttatct gttccaaaat
     1081 aaacaacagg tgttggctcg tttccttcac tgtaccatgc aaaattcagt tcggaagctg
     1141 cgccgccagg tgttaatgat acctttgtaa aatctgtagc tgtctggttc cagtctgcta
     1201 cccacttctg ccactgttca gatccgccgg ttacagatga gtcattgtaa tgtgtggtag
     1261 cagcccatac ctgctgtccc ccgatcacca gtacacctgc taaaagtcct gctgcaattc
     1321 ttctcttttt catatccatt ccctttcttt tatattactt tttgttattt tttattctga
     1381 tcatggtttt agtataaaaa agccatgtaa aatctactac cagggaaatg tataggaaat
     1441 gtatgatttt tgcaaattga agtctgaaaa atgaatgtac cccagaacat aaaaagcgcc
     1501 ctggaacatg ctttttacat atcccagggt gcttcctgct aatttttatg catctgatct
     1561 tgaaaaggca tttttcatag aagaactcag ctcaaataac accttattca gtgtatttcc
     1621 tgtctctttc tttaacatag ctgccatttc ttcgttgatc tcttcccgga tcttttcttt
     1681 ttcttccggc gtgcctgctg cctgctgcgc cttatctccc ttgatgatca gtgcatgggc
     1741 tttagatccc actgccaggc ggtagcgctc aatataaata gaagtctttg cataagctgc
     1801 atctgccata gctgcgatca tacggctggc ccagtagaaa ttctcggtgg aaacctctgc
     1861 tgtggtattt gccatatact ccggtgtctt tgatacctgt ggataaaatg gcactaatac
     1921 attaaatgga ttggaagcaa aggcaaccca ctggattacc tgaaactcct ccggcatata
     1981 agggcggatc tggatcagtg acagcagatc ggtgcggttg atgccgatag cgcggtattt
     2041 attgcggtat accgggtcag agtgcttcgc atatggatca taaggtgttc cctgaaaatg
     2101 agaggacagc acatatttta catcttccac tgtgatcttc ttttccggaa ccatgcacca
     2161 tggcagatca tctgactccg gtgtatagtc cgcgtcttcc ccttcccatt tcatggtatg
     2221 gggattgaaa tagcgaagac cgaaccaggc tcttggggta ttgtatacat ggtcagaatc
     2281 ctcatggctg ccaaaagcat cccgcgggtt aaagcagtca tcaaaggaca gatccagatg
     2341 attttcttca ataaattccc tcatatctgc agaacacata tattccttct gctcaaataa
     2401 ggcatcttcc agatcaaata catccagtcc cagctggttt ggcattacca catagctgtc
     2461 atcaggtact cttctggcga tccagtggtg tccgccgatt gtctcaagcc accagatctc
     2521 atttgcatcc tgaaaagcaa tcccattttt ctcataagtt ccatactgct ccaacaggct
     2581 tcccaggcgc tttacacctt ctctggcact gttaatataa ggaaggacaa gaacaacgat
     2641 atcctcctct ccaataccac ctggaacttc tgcctttccg tcttctgccg gctggtacac
     2701 taccatagga tccgcaccca ggactctcgg gttggaggta atggtctcgg ttgcagtcat
     2761 tgccacattt ttctcattta caccgcaggc tgcccaaatt ccctgtccgg gaatggcatt
     2821 aggagctgcg ctgtagcgca tgggatgctc cggaagcggg atctccacat gagagatcac
     2881 agatttgtac accttcggct gctcctccgg atgtacgatc acatattttt ttgctgtaaa
     2941 atgattaccg cctgcatcgt catttctggc gatcatggta gaaccgtcat aacttgcctt
     3001 ttttcctact aatatagttg tacatggcat aatgtcattc ctcctgtcat attttcctca
     3061 gggatcttaa gcagttccag aagtattttc tgaaacagca gaaagccccc tgaaagtacc
     3121 aaagcagttc caatggataa aatagtcatc cagcagactg cctgcttcat cttcttttca
     3181 tttccggcac cgaaattgcg gctgaccgta agagccagac cattgttcat accgaaagca
     3241 aagttggtga tcaggctgta aagagctgcc gttgcgccaa tctcagccag tgcctggtct
     3301 cctaacatat ggcctgctat ggaagtatct gccagattgt aaaactgctg cagcagattt
     3361 cctataaaaa gaggtagcgc aaagcgaaga agaagccccg cagacaccgg ccatcacatt
     3421 taccatgcct gtaaaggaag catccattct gcaagatatg tcttattttt gttcatgatc
     3481 cacattgcag ccaggacact ttttctgtgt tcttatgcat tcttgcaaat acaccatcgg
     3541 gataattttt cttcctgtac ttctcgttca gttccggaaa atacttctcc atgtttttaa
     3601 gtcctctttt ctctcgtttt ttgtctggcg gacttttttg tctacttgtc agctgataaa
     3661 agtttaacaa aagtgagcat agaagtcaaa tttttccaag atcttttgct attttccaca
     3721 actgtctgca aggttcctgg taagttccga agttttccat acagcagatc accttttcac
     3781 agtttttaag gacctttacc gcagtttcaa aggtcgcatc tgagaccgat tcaaaggctt
     3841 tttcccgaat caccacagaa gccagtgcct ctgccaccgg atattccctg tcgttttccc
     3901 acaggatacc tgctgcaaag ggaattccct ctttttgcag ctgccgatac accgggattc
     3961 ctgttccatt tccgccaatg acaaacacct tcggctctcc ttttacagct tccaactcta
     4021 cgctgccata atctgtaaga taggtgcctt tttcgatctg aaacagttct ttgatataat
     4081 ctccactgaa aatttcctcc ggcgtaccaa agcggccaat ggaattatcc ccttttacac
     4141 agataatatg atcggatatc ttctgggcca gatccagttc atgcagagac ataagcactg
     4201 ccactttctt ttcccgcacc atatttttaa gaagatacaa aagttccagt ttgtgccgga
     4261 tatctaaaaa tgcagtcggc tcatctaata caatgacctg tggttcctga cagatggctc
     4321 ttgcaagaag gactctctgg cgctgaccgt cactgaccgc ctcaaagtcc ttttctgcca
     4381 gttccagtcc ctggaccagt tctaaagact cccttacgat ctgccagtct ttttctgtca
     4441 ggatccccat tttccctgta taaggatacc gccccatact taccacttct tcacaggtca
     4501 tccgctctgt tttcacccga ccggtcatca tcacagacat acgaagggca atctcttttt
     4561 ccttcatggt ccccatagag gttccatcca gccacactgc cccttttacc agtttcagct
     4621 gaccgatcag gctctttaat atagtagatt ttcctgcgcc gtttggtcct atgagagtca
     4681 gtatctctcc cctgtttaca tgaaggttta tcttttggat caatggcagg ttatgataac
     4741 ctacgcttat atcttccgca caaatataag aactgcttct ttcttctgcc atcatcctgt
     4801 caccttcttc ctctggtctg tatcatcata taaataacca ccggcgctcc aaacacagct
     4861 gttactgtac tgatacttaa ttctaccggc gaaaacaggg tccttgcaag ccggtcacaa
     4921 aagctgcaaa atactgcccc gcctaaaaag caggcaggta tcataataat cggctttgtt
     4981 gttttaaaca ggctttttac cagctgtgga acagcaatcc ccacaaagga gaccggacct
     5041 gcaaaagcag taacgcaggc agaaaggata ctggacacca ggatcagtgc caggcgaaac
     5101 gcctttatgt tcacacccat actggctgca tagatctctc ctaactgata tgcccctgca
     5161 ggttttgcca tcaacatggc acagatcata cagaaaaaga tcaccactgc cataaccgaa
     5221 atattttccc aggtaatgcc ggaaaaactg cccaaagacc agttatgcag atttacgata
     5281 ttagagtcat ctgcaaaggt aactaccaga tccgtaactg cggaacagat atagctgacc
     5341 ataataccac agatgacaag aatagacata ttgttcactt tattggcgat caggatcaca
     5401 aaacccatag aaagcatggc accaataaaa gatgccaaaa tcaatacaac ggaacctgcc
     5461 accagccctc ttcccagaag aaatatcatc accagggctg tcatcagttt tgcaccggaa
     5521 gaaattccca gcacaaaggg gcctgcaatg ggatttgcaa aaaaggtctg aagcagaaat
     5581 ccggaaacag acagggcgcc gcctaataac atagctgcca gcactcttgg gaaacggata
     5641 tcccagagga tccttgcctg tgtactttca tccggtccct gaaacaaaag ccgcaacaca
     5701 tctttcggtt ctacacttac acttccggaa taaatattcc agaaaaaaag caggaccagt
     5761 gccacaacca ggagtaaaaa tccccctgca atatggatcc gcttttttgc tgttcccgtc
     5821 atttctctgg tcttcaacct gtgtttcccc tttcttattt cagttgatgg atataatgca
     5881 tctgtccttc atctggtgtc tcttctgtca gcatcctgtg tatatcctgt atcatatttc
     5941 ccagtcccat agtctcctgg aacaggttct ttcctgtaca ccacacatgt ccctctttaa
     6001 ctgccttaaa atctgctaac aatgggcttt ttgcaagaaa ttcatccatt gtggtcagtt
     6061 caccgtcaat ggtactgtta taaataatgt aatctgcgtc ctttgccttt gcataaaagg
     6121 cttccatttc catattcatg gtagaaagag cgttttcctc ttcttcctct gcactctggg
     6181 gaacataggt gccacctgcc atagtgatca tttttgccac ataatctccc ggcttacgca
     6241 cattggcact gcccttggat gtaatgtaaa aaaaggctac tgtttttccc tggtttgtct
     6301 gatccgaatg ttcctgtcct tcatccgcct gaacagaaac attttccagt tctttgatct
     6361 ctttttcaaa taattcctct gccagctgct cttttcctgt aagtaccccg tacagcttga
     6421 tccattccat ccttcctaaa ggatccgcct catagctgga acgctctacc aggaccggaa
     6481 ctcccagatt ttccagctgc tcctttacct gtggacaatg atagatcatg gtagactcaa
     6541 tggcaaggga gcaggactta tctacgatcc gttcataatc aggagcgctg tattttcctg
     6601 catattccat ggttccctct tccagagctt ttttcggttc ctctaaatac cagccggaac
     6661 ggtctgtacc ggaaagggtg atctgatcca ctgcatccaa atggcagaaa aagtccatag
     6721 cagaagtagc tgccaggtac atattcgtta aaggctgcaa aaggggcgta atatcttccg
     6781 ggagatcttc cggtaccggc tctccttccg gcactaccag gaatttttca tcttctccaa
     6841 taatgaccag ggcatagttt tcttccccat agtaggtgac agaaaactga tccgcatatg
     6901 aaagatccat acttcctgtc tgcgttagtt ctgaccagga aagggatttt tgccccgttt
     6961 ttcctgtctg ctcctgtgct gtaggggaag taactgtatt ctctatattt tctgtctttt
     7021 gtccgcatcc agcgcacatt cccagaacgc agacagtcag cgttcccaga agaaatcctt
     7081 ttaatagttt cttttttatc tgttttttca tctttctttc ctgtgatctg ctgcctgccc
     7141 gttactgtgg ctgaatactt tcagagtcaa agatcagtgt atagtcgatc tcatgaggca
     7201 cactcatggc tgtggtatct gcgatcactt tctgtttctg gtcaaaaaca gtgaccggga
     7261 tctcaaagga agaatttcct tctgtattta caggctcata cttttcttct cccactttca
     7321 tataatcata atgagagctg ccccaggtga tcacagccgt tgcctttcca tcctggatct
     7381 ttaactgcgt tggcgtttcc acagttgtac ggccggaacc gccttccagt ttcacttcca
     7441 ctgtgtatgt tccgtcttcc aggcccaggc tttccacagt tttcatcttc ccctctttgt
     7501 aggcttccat tggaagggaa gatgcccgga ctaccagggt cctgtcatac caggtttctt
     7561 tctttttgct gtaagctgca cagtcaattc ctttgtctaa ggcttctacc gggatctcaa
     7621 atacattctt tccatctgca ctctcttctg cctgaatgta atctccttcc cctgcttttt
     7681 ctgcctcttc cccagttccc ataaatactt ttccatagcc ggaaccgctt aaggtcagct
     7741 ttgcgctcat ctttccatca gcaaccgtca gttctgcctc tgtaatgtgg aacatggaag
     7801 agctggagtc cacctctacc ggataaacgc cgtcttttaa cgcatctgct tcaacaggca
     7861 ccatatcatc tgttactacc tgcactgtag gagccgtttc tttttctgtt tcggcacctg
     7921 ctgcaccaga tgtttctgca ttttcttttg tatctgcctg ttcgcttaca gcctgggaag
     7981 ttgccccgct gtctgtcttt tgtccgcatc cggaagacat ccccactaaa ccaacgatcc
     8041 cggcacacag tatatatttt cttattttca tctttttacc atctttctca ctgtctatag
     8101 gtccactgcc agacacatca ccggcacatt tcccatatgc attgcaacaa aaggcagcca
     8161 gaattttctc tgaccgcctt ttttatttta acttagcatc ctaatgcaaa atcgattatt
     8221 ttgcaagact gtcaacagct gcctttgcat gttctacaaa cagctgctgg atcggctcta
     8281 actcacctaa acctcttacc aggcaggtta cttcataacc agcttcttca aaagtggtct
     8341 tccaggaacc ttcttcatca cctgccatat cgttgtttgc gtgatcaccg gcaacgatca
     8401 ttaaaggctc taatacaact ttcttatagg aaccttcttt tacctttgct actacatctt
     8461 ccagacttgg agttgcttct acagtaccta catagtagtg ctcaaagcct tcctctgtca
     8521 gcatatcctg catcttggca tatacctgat tggagtcagc ctctgtacca tgacccataa
     8581 agcagatcgc ggtctcgcca tcatcatatt ccttggttgc gtctgtaatt gcctggatca
     8641 ctgccttaaa gtcatcatcg gatgtaagta atggttcacc tacaacaacc ttctcaaagg
     8701 agtctgcatt ttctgcaatt tcatttacca gatctgtata ttcaagacca ttcatcagat
     8761 gggttggctg aacaaccaga gtctttacac cattatttac tgcacggtcc agagcttctg
     8821 ttacattatc aatagtaaca tcatccctct tctttacatg gtcaataatg atctggctgg
     8881 taaaacctct tcttacggaa aaatccggct ctgccttttc gatggcatct tcaatagcac
     8941 caatggttaa acgacggctg tcattgtagc tggttccaaa acttacaact aaaagttctt
     9001 tttctccgat ctcatcctga tttcttgtgt tgtccaatgc tgcatctcct gtatcgtagt
     9061 tttcttcgtc ctcttcttca gcagcagttt tctctttctg ggtctcctct gctgtggtct
     9121 cttcttttgt ttcctcagca tttgtctctg ctacagaagc ggctgttgtc tcctttgcgc
     9181 ttccagagca tcctgctacg gtacctgcag ctactgctgc cgctgcgaaa agcagcattg
     9241 cttttttcaa cttcattttc attttcctcc ttgtcctaag acacataata atatgtatcg
     9301 atccggaaga aattcccggc agggcgcagg agtccatttt cctgtgtgag tcagaaatcc
     9361 tgatccatga taacacgact ggaacaattg ccatgatcat ctttcaggac cggcacgatc
     9421 aattactcgt gacccggaat gaaccttctc ctgtaccaac aaatggcagg tttcctggct
     9481 tgctgctttc tacatacaaa actgccttcc cggtttccca gtggcggata ttctgtcctt
     9541 tctatccacg ttttatatgc atgggatctg gatgataact ttccggatct cctttacagc
     9601 ttacagtgac gagatcgcgc aggtacttcc cctgcttccc ttttacctct ttataaatag
     9661 acacgaccgc tggtcagcgg ctggtccata aacaaagagc accatttgct cttaactcga
     9721 tataaagcta tcctatcacc tattagttta aaaagcaact tattttactg tcacagctga
     9781 atatagcact ttatacccag gaacagatca ggcttaagtt accctcaaaa gagcaggtcc
     9841 caaaatcagc aggaatgata aaatgatctc cctttttgat ctttctgccg tttatgcttc
     9901 cttctccttc aatcacactg gcatttacaa aaccagcctc aaaaacatgg tcccaggttc
     9961 cgtggatatc gtatttttct actgtataat aagggcatgt ttccagatgt tccaggtcag
    10021 ctccttctgt atgggtgatg cttctctggt cctttgcagg ctggaatgga gttttgatca
    10081 catccagact ctgttttaca tgaagttccc taagctttcc gttccatttt ctgtcataat
    10141 cgtatacacg gtaagtaatg tcactggact gctgggtctc taaaataaca gtaccgcctt
    10201 tgatggcatg gacacagcca ggatcgatct gataaaaatc gccttttttt accggcactt
    10261 cacggatcag gtctttccag cgcttttcct ctaccatctg cattaattct tctttgtctt
    10321 ttgcatgatg gccgataacg atctgggttc ccggctcact gtctaagatg taccagcact
    10381 ctgtttttcc cagagagccg ttttcatgtt cttttgcgta ggcgttatcc ggatgtacct
    10441 ggatgcttaa gtcagatttg gcgtcaatga tcttaataag aaggggaaat tcttttccat
    10501 attttccatc ttcatttcca aacaattccg gatgttcttc ccacaatttt gacagatgct
    10561 ctcccttata agtgccttct gcgatctcac agtctccatt tttgtgggct ccgatgcccc
    10621 agcactcccc ggtactgtcg cttggtatct catacccaaa atcatcccga agttttctgc
    10681 ctccccagac agcttctttg aaaaccggtt tcataaacaa taattccttc atatttatat
    10741 tcccctttct gctttgcagt tgcttgtttt gccatgcata tcaaaaacgc attagcaacg
    10801 cgtttgaaga tttccctgcg ctcattatat tatagcgcat tttgttagtg tcttcgatat
    10861 aaatttgaca ttttccccct tttgcttata atagaactgc ttatgaaata cttaagactt
    10921 gcaacaaatg aagaggtgca tcatggaatt actacaatta cgatattttt tagttgcggc
    10981 ccagtaccag catatgacaa aagcagcaga acacttacag atcgcccagc cggctctttc
    11041 ccaggccatc cacaggctgg aagcagaact gggtgttccc ctgttcgaga gaaagaaccg
    11101 cagcattgaa ttaaatgatg aaggaaaatt tctgcaaaaa cggctgatcc ctattttaag
    11161 cagcttagac cggctgccgg aggaactgcg aaaaggaaag gaagctgcca cccacactgt
    11221 acatcttcgt ctcctttctg cctctgccct gatcaccaac cggatcattg cctacagaag
    11281 cctccgcccg gatgtaaatt tccagctgta ccagatacca ggacaggaag acgattttga
    11341 cgtatgtgtt acagcccgga gagccgatac tgccccaaag gaaaatgacg atgccacggt
    11401 tatgttagaa gaagacctgt atctggcagt gccatcccat tctccctatg gatccaggga
    11461 gtccatccgt ttaacagaca caaaagatgc cgattatatc tgccttggcg gttccaagcc
    11521 catccgccag atcacaaaca gctattttat ggaagctggc tttactcccc atatcatctt
    11581 tgaaagcgac actacggagt ctgtgcgaaa cctgatcgca gcgggaatgg gcatcggttt
    11641 ctggcctgcc tgctcatggg gacctcttac tactaatttt ggtggttcgc ctggtcattt
    11701 tccggtaaaa cttctgccca tcacagacca gaaatgccgc cgccagatca tcctgacccg
    11761 gtccgaaaaa ggaaagaaaa gccctattgt agctgatttc tgcgattttc tggtagaaca
    11821 taacaactgg ctgtaactat aaaaaatata taacgcaaaa acagcgtacc ttccggcgcg
    11881 ctgcttatgt gatctataat gtgatctatt ataatccatg gtattcactg cccttatgct
    11941 ggctggcttt cagacggctg agggcacggg ccatggatgc ttttgacata taatattctc
    12001 tgatactctg tttctgacga agctgttcct gggcccgttc caaagcctgt ctggcccgga
    12061 cttcgtcaat atcttccggc cgttccgctg tatctactac aaccgttaca cggttatttg
    12121 ctacctgtac gatcccaata cctacaaccg cttcccggta ctggttttcc ccgggaacgc
    12181 gaaaccgcag ttctccttcc tggattgcca ggatcatagc ctctctgtga ggaaggatac
    12241 ctacttcccc atcatgagcc ggaaccacca gggactcgca gtttccatta taaaatacat
    12301 ggtcactggc tatgacctta agccagaatg ctgccattat gacgctcctt cctgcatcgc
    12361 cttcgctttt tccactactt cgtcaatggt acccacatta aagaaggctg cttccggata
    12421 ttcatccatt tctccgtcaa tgatcgcctt aaatccacgg accgtttcct ttacaggtac
    12481 atattttccc ggaacaccgg taaagttctc tgccacatgg aatggttggg ataaaaatct
    12541 ctgtatcttt cttgcacggt tgactgtcag cttatcttcc tcggaaagtt cctccatacc
    12601 caggatggag atgatatcct gcagttcttt atacttctgc agcatctctt ttactttatt
    12661 ggctacttca taatgctctt ttcctacaac atcctcttcc aggatacggg aagaagactc
    12721 cagaggatcc actgccggat aaataccctg ttctacgatc tttctggaaa ggactgtagt
    12781 tgcatccaga tgagcgaaag tagtagcagg cgccgggtct gttaagtcat ctgcaggcac
    12841 ataaacagcc tgaacagaag taatagaacc attctttgta gaagcaatac gctcctgaag
    12901 tccgccaagg tccgttgcca gtgtcggctg ataaccaact gcagaaggca tacgtccaag
    12961 aagggcggaa acctcagaac cagcctgtac aaaacggaag atattatcaa tgaataaaag
    13021 cacgttctga tgctttacat cacggaaata ctctgccatg gtcagtcctg tttctgctac
    13081 tctcatacgg gcacccggtg actcgttcat ctgtccaaat acaagggctg tcttctttaa
    13141 aacgccggac tcgctcattt ccgtccaaag gtcatttccc tcacgggaac gctctccaac
    13201 accggtgaag attgaatatc cgccgtgttc cgttgcaatg ttggtgatca gttcctggat
    13261 cagcaccgtc ttaccaacac cggcaccgcc aaacagaccg atcttaccgc cctttgcata
    13321 aggtgctaac aggtcaatga ccttgatacc tgtttccagc atttccacaa ccgggctctg
    13381 gtctgcaaaa cttgggggat ctctgtggat gacccatttt tcttctccct ccaggctctc
    13441 accgccatct aaggtgtccc caaggacatt aaacagacgt cccagtgtct tttcaccaac
    13501 aggaacagca attccctgtc ctgttgcttc tacttccata tctttatata aaccttcact
    13561 ggaagctaac ataatacagc ggactacatc tccgccaata tgctgggcta cttccataac
    13621 gcaacgcttt ccattgttca ttacagtcag cgcatcctgg atatgaggaa ggtttccatt
    13681 ttcaaaagcc acatctacta caggtcccat tacctgtatg atctttcctt tattcatgat
    13741 ttactcctta tctccgcttc ttccgctgtg cccttgcacc gcctaccact tccgtgatct
    13801 cctgggtaat ggcagcctgg cgcacccggt tatattcaat ggacagctca tttaaaagct
    13861 ctgccgcatt gtcgctggca gactgcattg ccatcatacg ggcattctgc tcactgcaaa
    13921 atgactctac cagggcaccg aagataaatc ctgttacata gtttggcacc acatggtcca
    13981 tgaccgctgt aggggatggg accatctgga tctcttcctg atggatatcc gcaggaatga
    14041 ccacattgct gaaatcttcc tgcttcaggg gaagaagctg ctccatctgt gcttccacct
    14101 tcatgctgtt tatcatctgc gtataaatga tccacacctc atccaaacgt ccttccagat
    14161 aatcctcgat cagggaagag gcgatcagac gggctctgtg catggtaggg ttctgcaccg
    14221 tgtagtggaa ctgttcttct accggcacat gtcttgcagc aaaatactgt cttcccagct
    14281 ctcccacgac atacagacgg tttttatcac ttttttccat ccattcatgg gcaagtttca
    14341 gtacattgtg gttataagca cctgccagac ccttatcacc tgtaatgact accaggccac
    14401 ggacacggtc ctcttctttg atctcttttc tggcgttaaa ataaggatgc tctgtatccc
    14461 ccatatggcg caggatacgc cggatcaggc tctgaagggt gtaaaaataa ggttctgtat
    14521 cttccagaag ctttttgctt ttcttcagct tggttgaaga gatcagatac atggcattgg
    14581 tgatcttacg tgtatcctga atacttttta ttcggttttg gatctctctt gcgtttgcca
    14641 tactgcctcc ttaaactcct tcgctgcctc tacgatccgt tctgccagct catcgcttaa
    14701 gactttcttt tccgtgatct catttccaat ttcaggatgt ttgtcctcaa accaggcaag
    14761 catatccatc tggaactttt tgatctgttt tacatccaca cttaccatta ctttgtgggt
    14821 tgctgtacac agggtaatca cctgctctgc catggaaagc ggacgtccca aaggctgttt
    14881 taacagttcc atcagacgtt ttccataagc cagctgtgct tttgtcgcct catccaggtc
    14941 agaactgaac tgggtgaaca cttccatttc acgatactgt gccaggtcga tacggatggt
    15001 accgctggcc ttcttcattg ccttggtctg tgcagcaccg cctactcggg atacagaaag
    15061 acctacgttt actgccggtc tcattcctga gaaaaacagg tcgctttcaa ggaagatctg
    15121 accatctgta atagatatta cattggtagg aatataggct gatacatctc ctgcctgtgt
    15181 ttcaataata ggaagggctg taatagaacc tcctcccagt ttatctgaaa gacggctgga
    15241 acgctccagc agcctggaat gaagatagaa aacatctcct ggatatgcct cacgtcccgg
    15301 agaacgctca agcaacaggg acaatgcacg gtaagctact gcatgcttgg acaggtcatc
    15361 ataaacaatg agcacatctt ttccctggcg catgaaaaac tctgccatgg cagttcccgc
    15421 ataaggggca atatattgta aaggcgccgg atcactggct gtagaagcca taacaatggt
    15481 gtaatccatg gcgtcatttt tctttaatgt atttaccagc tttgcaatgg tagatgcctt
    15541 ctggccaatg gcaacataga tacagatcac atccttgccc ttctggttta aaatagtatc
    15601 cattgcaatg gaggttttac cggtctgacg gtcaccgatg atcagctcac gctgtccccg
    15661 tccgataggg aacatagagt cgatggccag gatacctgtc tccataggaa ctcctacgga
    15721 tttacgttct gcaataccag gtgcttcctg ttcaaccggc aggtaatcct cttcttttgc
    15781 aggtcccatt ccgtcaatgg tctctcctaa ggcattgaca actcttccta aaaaagccgc
    15841 tccaacagga acacctgctt ttttctttgt tctggtaact tttgttccct ctgtaatacc
    15901 tgtatctttt ccaaacagga tacagccgat ctcattttta cggatatcct ggaccatgcc
    15961 cttgactccg ttttcaaata taacgatctc tccatacatg gcatgatcaa taccatatac
    16021 aatggcaatg ccatcgccta cccagatcac agaaccagtc tcattaacct gtcctgcatg
    16081 agcgtcatag tcttcgatct cgcccttgat aatggagatg atctcctgag aactgattgt
    16141 actcatagta ataactccta tctgcctgca gtctgaaaca gccgctcaag ctgccctttc
    16201 aggctgtaat catattccac atctccggtc tttaagacga aaccgcccat cagttccgga
    16261 gcagcagcca tttcaagctg cacatccttc tttttatatt ccctgcacaa aaattccagg
    16321 atccctgcaa gctgttcctc atctggtctg gtcacataaa aaagactggc tgataaaata
    16381 cccgctccat ctaacagaca tttctcccag acctttatca tatcttccat ctgcccgatg
    16441 cagccttcct cacaggcctt ctttaaaaaa tgaaccattg tactggaaaa atcaggttct
    16501 ttccatattt ttccaataat ggcttccttt tttgtttcct gcaatgtggg atcatcaaga
    16561 actgccttaa gttctggatt ttcctggaaa atgcgcccgg tctcccggac cattttctca
    16621 ggtaccttta attcataaag aacggttccg tataatcttg cctgctcagt catgtttttc
    16681 acctgcgctt gctaaaaatg cctgataacg tttcttgtcg ctttccggtc ctgcctgctc
    16741 agacaaaagt tttgccgttg cagtcattgc cagacctgta acagaagcac gaagatcatt
    16801 taatgccttt tctctttcca gatcgatcat tttctttgca tcttccagct ttttagaagc
    16861 atctgcatct gcctttgaca ggatcttctc gtattcctct cctgcccttt cctttgcctg
    16921 ggcaacgatc ctgccagcct cttcatctgc tttttcaagg gatacttcat actttttctt
    16981 tagcagaccc gcatccctgt tagcagcagc cgctttatct aattcctttt ccaccatctg
    17041 tttcctctct tccagaatgg acatcagcgg cttcataagg aaatgcttta aaaacagata
    17101 cagcacaaca atgttgatga tcgtaaaggc aatattccaa aaattaatat ttagcatatg
    17161 tactgccttt ctacttcagg aacaggatga tcagaagacc gataacgaaa ccgtaaatag
    17221 cagttgcctc tgccagtgcg catccaagaa gaagtgcctt tgtgatcttg ctgtctgctt
    17281 caggctgtct tgcaatagca tctactgctt tggaagtagc cataccaatt ccaagtcctg
    17341 ctccaattcc tgtaattact gcaactgcag ctccaagtgc gattaatcct gacatgatga
    17401 tttctccttt tgtttaaaaa tttttgttta tttactcaat tgcttctttt atatataaag
    17461 atgtaaggaa tacaaacaca taagcctgta tcactccatc gaatatatca aaatacccgc
    17521 taaatggcag tggcaccact aagggcagca gatatttgat cagtgccatg accactactg
    17581 cgccaagcac atttccaaac agtcgcatgc acagcgacag cggccggata aacagctcca
    17641 gtacattaat aggagcgata atggggatcg gttccgcaaa acttttcacc cagcccttta
    17701 cacctttttt ccggatacct gcggcttcca ccagcacaat actcataaca gctaaagacg
    17761 cggtcacatt taagtctttt gtgggggatt taaaacctaa cagaccgatc atattggcga
    17821 tggctatgta gataacaacg gtgatcagat aaggaatgta atcttttcct tcttctccca
    17881 gaagatccag aaagaagtta taaaggaaag aaaaaccact ttccagtgct gcctgttttc
    17941 cgcttatttt acggatctta agcccatgca cccagatcag ggagccgatc atgataatgg
    18001 ccataatgat ccatgtgacc accaccgatt ccagtacatc gatcccgcca aataaaggaa
    18061 tggtaaatac tgtattacag tttaattcct ccatcaggga gtctaccagc tggtccattt
    18121 tttcactctc cttcctacct attttcttgt cttttttgtt tcgtccgtta ttatagccac
    18181 atgaattttc cttttcaatt ctgaatttct tattatttca taacacaaat gttatcaaac
    18241 taaatattgc ataattatta tggattattg tttatttttt ttggaacatt tcagaatata
    18301 tgtttagaag atgttaagac ttttttctac atgtttttaa atgaatacaa aaaaagatgc
    18361 ttctgttctt tcacttttta taagtaaaaa aacgaagcac cttatatctc tttattcatt
    18421 ttccggaaga gctcttccac tggcaccggc tttccaaaat agtatccctg agccagatca
    18481 cagcccattt catacagcat tttggcctgt tcctctgttt caaaagagcg ccggaggcgg
    18541 tctttcctat aaagtgaaaa agaatgtgcc ttggcacatt ctcgcgccga gcgcggtcgc
    18601 ttgcgacaaa cttcctcatc gcgcgagtgc gcattattgg caggtcgtgc ctttttgtgt
    18661 actagggagt tccaacggaa tttcctagta cataaaaaac tgccgcgaaa tattttctta
    18721 tttcgcgaca gtttcatact tttttctctc agatttgcgc tatcgcgctt ttctattaga
    18781 acagtcccgg gaacagggta gccggaatag ccagccagaa gttcgggaag attactaaca
    18841 gtaataatac tgcgatagag gataagaaat aaggaagagt tcccttaaat gcatcctcga
    18901 tggtaatacc actgatagaa gaagcgatca gcaggcacag accataaggt ggggttacca
    18961 ggccaagagc cagggtaaca acgatgatca ggccaaggat gatcgggctg atctgcagtg
    19021 aagtagcagc cggaagaatg atcggaacaa acaggatcat tgccggtaca gcatccatga
    19081 aagtacctac aaacataaag aatgcaacta ctacaagaag gaataccagt cttccgcctg
    19141 gcatattagc gaagaagttc tgtacaatta cattcaactg atagtatgct aataactcgc
    19201 ctaatgcatt tgctgtagcc agagcgaaca gagacaggga agacagcttc atagtctcga
    19261 tcaggatacg tggcaggttc ttaaccttaa tggtcttgta gaagaagata ccaactaata
    19321 atgcatagat acatgcaaat gctgcagact cagtaggtgt aaacaaaccg ctgacgatac
    19381 caccgatcag gatgatagga gtaagcagtg ctggcagaga ctgaaaagtt ctgcggcata
    19441 cttccttaaa cggaacacga tcacacttag ggaagttctt tctcttggag tagtacttaa
    19501 ctactgccat catagccaga ccaagtaaga tacctggaac cagaccggac atgaacagcg
    19561 caccggtact tacgttagcg atacctgcgt atacaaccat ggtgatactt ggagggataa
    19621 tggaacctaa tgtggaagat gctgcggtaa caccaacaga tgttcccttg tcatatccct
    19681 gcttctccat tgcaggaatg aagatcttac caacaccagc ggtatcagcc tgggaagaac
    19741 cggagatacc agcgaag
//
LOCUS       DBMDUA010000031        19324 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1713_length_19324_cov_3.586069, whole genome shotgun sequence.
ACCESSION   DBMDUA010000031 DBMDUA010000000
VERSION     DBMDUA010000031.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 19324)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19324)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19324
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1713_length_19324_cov_3.586069"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /gene="sstT"
                     /locus_tag="ACZPCL_04175"
     CDS             <1..859
                     /gene="sstT"
                     /locus_tag="ACZPCL_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HIR1161715.1"
                     /translation="AGQERYECRKNLVQSLVSANYIGILVWAVLIGIALRKASDTTKQ
                     SLNDISAALSKTVTWIIQCAPFGIMGLVYNTVSVNGLSTFTEYGKLLAVLLGSMLFIY
                     FVTNPILVFWCIRKNPYPLIFKCLKKSAITAFFTRSSAANIPINMEACAEMGLDKDTY
                     SVTIPLGATINMDGAAITITVMTLAAAHTLGIAVDIPSAIILSILATLSACGASGVAG
                     GSLLLIPMACSLFGISNDIAMQIVGIGFIIGVIQDSVETALNSSSDLLLSASAEFRQW
                     RLEGKEIKF"
     gene            900..2297
                     /locus_tag="ACZPCL_04180"
     CDS             900..2297
                     /locus_tag="ACZPCL_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1161716.1"
                     /translation="MLSTIETINQVVNDFIWGVPAMVCIIGVGFYLSCRTGFLQIRKF
                     SYAIQTTIGRMFRKRNASDGALTPFQAVCTALAATVGTGNIAGVAGAIAIGGPGAVFW
                     MWVSAVLGMCTKFAEVTLAVYYRETNKEGDLVGGPMYYIKNGLGKNWQFLAVLFSAFG
                     VLTVFGTGNATQVNTITTAVNSALLSCGLISEGAVKTSNLIQGIIIAALVALILLGGV
                     KRIGQVTEKLVPFMALLYIVLSVGIILFRIQALPSVIQAIFEGAFKPAAVTGGAVGSL
                     FMSMKKGVSRGIFSNEAGLGTGSIAHACADTRKPVKQGMFGIFEVFTDTIVICTLTAL
                     VILCSQVPVGYGQAAGAELTIQGFISVYGNWVSVFTAVAMCCFAFSTILGWGLYGARC
                     IEFLFSEKVIKPFMVAYSLVAILGATADLGLMWNIAETFNGLMAIPNLIALFLLSGTV
                     VKLTKEYFAGEGAGK"
     gene            2486..3511
                     /locus_tag="ACZPCL_04185"
     CDS             2486..3511
                     /locus_tag="ACZPCL_04185"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIR1161717.1"
                     /translation="MEKKNLDWANIGFSYMPTDMRYVSNYKDGAWDEGTLTADSNVTI
                     NECAGILQYCQECFEGLKAYTTEDGSIVTFRPDMNAERMMDSASRLEMPVFPKEKFLD
                     ALDQVVKANAAWVPPYGSGATLYIRPYMFASGPVIGVKPSHEYQFRMFVTPVGPYFKG
                     GAKPITICVSDFDRAAPHGTGHVKAGLNYAMSLHAHEVAHANGFDENMFLDPATRTYV
                     EETGGANFLFVTKDGEVVTPKSDSILPSITRRSLVYVAEHYLGLKVTQRPVKLSELGD
                     FAECGLCGTAAVISPVGKVVDHGKEICFPSGMDEMGPVIKKLYDTLTGIQMGRIEAPE
                     GWIRKIC"
     gene            3590..4012
                     /locus_tag="ACZPCL_04190"
     CDS             3590..4012
                     /locus_tag="ACZPCL_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HIR1161718.1"
                     /translation="MKNGKCLLCTVCAGIVALCLLLSGCSILGKKEEKVRDLTITPVS
                     DSGLPQELQTLIKQKTSAPFKMTYTTDENLYIAVGYGPQEGGGYSICVNELYLTGNSV
                     VIATELKGPENGEDKGVESSSPYIVVKTELLDLPVVFQ"
     gene            4219..4857
                     /gene="cysE"
                     /locus_tag="ACZPCL_04195"
     CDS             4219..4857
                     /gene="cysE"
                     /locus_tag="ACZPCL_04195"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIR1161719.1"
                     /translation="MIFKDIWLDIKAVQERDPAARSALEVLLLYQGIHALIWHRFAHW
                     FYKHKMFFIARLISQIARFFTLIEIHPGAQLGHGILIDHGSGVVIGETAVVGDNCTIY
                     QGVTLGGVGTQKGKRHPTLGNNVMVGAGAKILGAFEVGDNCSIAANAVLLKPLEDNVT
                     AVGIPARPVKKDGVTLPKRKETMTLEEYETMKERMLQMEEEIRQLKEKLNKQ"
     gene            4924..6513
                     /locus_tag="ACZPCL_04200"
     CDS             4924..6513
                     /locus_tag="ACZPCL_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 and LysM peptidoglycan-binding
                     domain-containing protein"
                     /protein_id="HIR1161720.1"
                     /translation="MELIKKQIHMNRWKGNAVTQITLDEDFIVPDTLEDMEQVILEQA
                     HIQGENVKTQEEKITVKGKLEFQVLYRKENGGLETLGGNIPFEETVNVPGLAEGDHTG
                     LNWILEDMKTDMINSRKLGVKAVITVEIHTEGETSQMAASDLKETGTAGSFSSQTEEM
                     PPEVKMGSISPVILALHRKDTYRIRENTVLTGGRPDIENLLWKDISISSQSVKPLDGR
                     LHLEGQLTLFVIYETEEENMPVQWMEEVIPFSGDIEIAQAHEGQIPMVSLRLAHGEVE
                     AKPDPDGQMRELDLDVVLDLDIRLYEEKEEMLLKDIYSNSCQITPEKNEMGYDRLIAR
                     NVCRPRIGEKIHLGQQERILQICHTQGMVQLDEIEPEENSLDIEGILDVTILYLTSDD
                     SAPIQSVSRQIPFKCSAQAAGITKKSIYQLDAGVDQLTAVMTGADTVEVKGVAALDFM
                     ILEPVQETVITGIKEEPLDLKQLQELPGIVGYIVQEKDSLWDIAKKFHTTVENIVTTN
                     ELPGEQVKTGQRLLLVKEVGV"
     gene            6612..7526
                     /locus_tag="ACZPCL_04205"
     CDS             6612..7526
                     /locus_tag="ACZPCL_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HIR1161721.1"
                     /translation="MDLNFRPVEAEDMSRLRPFYGLRPNRTCDSVFLDSFLWKDYYKV
                     ECAVSEDKAVLWLMEKDGILSSAMPLCKEEDLPYFFYQMVDYFDNVLHKPFYISLADA
                     EAVEYLNLDPEKFEVTELDDLKDYLYDGNAMRTLAGKKLHKKKNRLNGFLKTYEGRYE
                     YRRICCSDRQEVWQFMEKWRQQKVESYENEISLDYEVAGIHDILKNCSAFNVPMAGVY
                     IDGELKAFTVGSLNERENMAVIHIEKADPEINGLYQFINQQFLVHEFPDVALVNREDD
                     VGMPGLRKAKMSYYPVDFARKYSVKKKY"
     gene            7594..8667
                     /locus_tag="ACZPCL_04210"
     CDS             7594..8667
                     /locus_tag="ACZPCL_04210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1161722.1"
                     /translation="MVRYLEKSEYGKCRPLWEEAFPEDSKSFADYYFSEKLPGSKVLA
                     IEGGKLLTMAHLNPYTISVNGKKWELPYIVGVATAAHQRHKGYMRQVLNQMLRDLNQE
                     KKPFCFLMPAAEAIYRPFQFAFIYDQPVWKPEDEPEKDLEKVPVDLAEKSEELAYWLN
                     NWLEKRYEVYAVRDRAYMELLKKELESEAGEVTGLYEKDGKLHALEAWWGLGKREERF
                     YYSISEIKPSDMHPAIMVRITDVRSLLEVIGLNENAPGDKFQAVLSIKDPIISENEGC
                     WLWKLGKNGSTLERMKGLGLQTEADEAEQIPSSSEVLEINIDELAQWIFGYKTLEETL
                     GVMPPFWTEYVRTLQGVFLDEVV"
     gene            8680..9435
                     /locus_tag="ACZPCL_04215"
     CDS             8680..9435
                     /locus_tag="ACZPCL_04215"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HIR1161723.1"
                     /translation="MDEKWQQLKDWINESDNIVFFGGAGVSTESGIPDFRSVDGLYNQ
                     KYKYPPERIISHSFYIQNPEEFYRFYKDRMLFPDAKPNAAHKALAKLEQEGKLKAVIT
                     QNIDGLHQMAGSKEVLELHGSVHRNYCTRCGQFYDLDYVRNSEGVPTCTCGGRIKPDV
                     VLYEEGLDDRTIQKSIDYIRNADMLIIGGTSLVVYPAAGLIDYYRGNKLVLINKSVTS
                     RDSQADLVISDSIGKVLGTVVGNDNGSGRSKEK"
     gene            9440..9649
                     /locus_tag="ACZPCL_04220"
     CDS             9440..9649
                     /locus_tag="ACZPCL_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HIR1161724.1"
                     /translation="MPGERLNYEVGDIVKLKKPHPCGSSEWEILRVGADFRLKCTGCG
                     HQIMVARRLVEKNTKGLKKKDEITG"
     gene            complement(9639..11459)
                     /locus_tag="ACZPCL_04225"
     CDS             complement(9639..11459)
                     /locus_tag="ACZPCL_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrpR N-terminal domain-containing protein"
                     /protein_id="HIR1161725.1"
                     /translation="MIKKIRILGIAPYKGLATLMKQCALQYPEIEFTAYAGSMEQGLA
                     LAKRYSEHYDVIISRANTAKMISQSVHIPVIDIGIGYYDVLRCIKMAQNTGTKFALLG
                     FQSLTVIAKNLCDLLQIHVDIFSFSVDNWKGSGDLLDRMKEQGYKTVVCDMIPYDHAK
                     MIGLTPILLTSSAESVKQAMENAIGTWQQYQKLCNSNAMMQSLIRSSSNQYLILDLEG
                     RCHYSTINDEKEEEFIQSLQKELGKCRTSSRRSFFVTLGNQLYSVRSSLAEEGDFPYI
                     IFRIMLSKIPLSHSKYGITIMDKEQALQSFIESFYSNTELSRSAAAAMDQSGSSSVPL
                     MITGEIGTGKDRVAYLHYAKSQFNDEPLYVVNCSMLNDKTWNFLINHYNSPFTDNGNT
                     IYISNLGVLSHPRQKQLLSIILDTNLHIRNRLIFSCTQAKDGHMPHAALEYTNMLGCV
                     PLPIKPLREQKNDIVPSAALYIDTLNQNLGRQVVGLEDGAAKLLMEYDYPCNRTQFKR
                     ILKKAVLETSTAYISRDTIEKILKEESAFFPDDHEAHMITHSKAATEKEGFSLNLEQP
                     LDRINQDIVQHVLNSCNGNQTAAAKKLGISRTTLWRYINR"
     gene            11636..12856
                     /locus_tag="ACZPCL_04230"
     CDS             11636..12856
                     /locus_tag="ACZPCL_04230"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HIR1161726.1"
                     /translation="MEERYLIIADDFTGANDTGVQLRRRGFPTEVLFCGKPMGADRSI
                     VIDTESRTVHPDHAYEIVSHALEDVDFDSFRYVIKKVDSTMRGNIAAEIKAVDEHFKP
                     ELMIFAPALPDLKRTTVDGVQCLNGVEICRTELASDPKNPVVEDNLVRMLGRYYEEKV
                     ILKRLPDVRSDDLDFTEGRIYVCDADCNDDLKKVLKAVAKTGKKVLYIGTAGLADNLM
                     ELERPSLPALGVVASVSTVTNRQMKYCEEAGIKMVKLPMHDILSGKEDTESYLKEVVD
                     SLKAGKDTILLTNTAYDRSELELSFEAGEALGLGPVETGDKVRSIVGRLAMEILEQAK
                     VSGVFLTGGDTALGMLMNIEADGSQILSEIRVGIPLVRVKGGKYEGMKLVTKAGAFGA
                     DDAAAFALRKIKEA"
     gene            12912..14189
                     /gene="larA"
                     /locus_tag="ACZPCL_04235"
     CDS             12912..14189
                     /gene="larA"
                     /locus_tag="ACZPCL_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HIR1161727.1"
                     /translation="MKEFLLPYGKEKLTAKIEDEHLAGVLLSELHSYKAPKSGAELVQ
                     DALEHPIGTPRLCDMAVGKKKVVVISSDHTRPVPSHIMMPLILAEIRKGNPDADITIL
                     ISTGLHRTTTKEELAAKFGPEIMANEKIVVHDCDDKDNLVYLGKLPSGGNLIINRLAA
                     EADLLVAEGFIEPHFFAGFSGGRKSILPGVASRETVMYNHNSGFIADPHARTGVVEGN
                     PIHNDMLYAARAARLDFIVNVVIDAAHDPIFAVAGDCDLAHRVGREFLASKCQVDAIP
                     ADIVISTNGGYPLDQNIYQSVKGMTAAEATVKEGGVIIMLSKAADGHGGKYFHETFRD
                     EKDLNRMMKTFLDRKPEETIIDQWQSQIFARLLLKATVVFVSSCDDQLVEDLHMVPAH
                     SMEEALEKAKAIVKKDDYKVTVIPDGVSVIVRE"
     gene            14341..15471
                     /locus_tag="ACZPCL_04240"
     CDS             14341..15471
                     /locus_tag="ACZPCL_04240"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase family protein"
                     /protein_id="HIR1161728.1"
                     /translation="MKFVLIPDSFKGTLSSEQICEIAKEKVEKHFPGAETACVPVADG
                     GEGSVDAFITALGGSKVTVKVKNPYFEDMDSYYGLINEGKTAVIEMASCAGLPLVEDR
                     KDPRKTTTYGVGQLILAAAESGVEKIIVGLGGSSTNDGGCGAAAAVGVKFYDKDGKEF
                     VPTGGTTADICKIDLSGKAEILNKVEIVTMCDIDNPMYGPTGASHIFGPQKGADEAMV
                     LELDEGIKNLSRAITEAIGKDISEVPGTGAAGAMGAGMIAFFGSRLQMGIQTVLDTVK
                     FDEMISDADYIITGEGKLDSQSLRGKVVIGIAERAKKQNKNVIAVVGGADDAEIDKAY
                     EMGVTAVFPINRLPQDFSISRHHSKENLAYTVDNVLRLIAAK"
     gene            15504..16460
                     /locus_tag="ACZPCL_04245"
     CDS             15504..16460
                     /locus_tag="ACZPCL_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HIR1161729.1"
                     /translation="MNILKTVKKIPGGLMVVPLLLGAIINTFFGGTIWTMFDGTFTTY
                     LWKSGAMPILAVFIFCNATTINFKKAGVTVYKGVVITAVKVGVGIIIGLICGKLFGEA
                     GFLGLSTLAIVGALANSNGGLYAALAGEYGDATDVGAVSILSINDGPFFTMVALGAAG
                     VAKIPMSVLVGCIIPVIVGCIMGNLDEDIRKFCEPGASMLIPFFAFPLGAGLNIMNLI
                     KAGGPGILLGVGCTLITGLVGYLVYKLLRMDHPEVGGAIGTTAGNAAATPASVAAVDA
                     TLLAVSEAATAQITAAIIVTAICCPILVSWLHKLEDKRRAAK"
     gene            16707..17711
                     /gene="pdxA"
                     /locus_tag="ACZPCL_04250"
     CDS             16707..17711
                     /gene="pdxA"
                     /locus_tag="ACZPCL_04250"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR1161730.1"
                     /translation="MGDPASIGPEITVKAFADKSLYDLCNPVVVGDACVMEAALPIVG
                     HTEMKIHAIKDVSEAKYEYGTIDVLDMGLVDMAQLKRGEVSAMCGDAAFKYVTKVIEL
                     AMDKKVDATVTNAFNKEAVNLAGHHYSGHTEIYADMTGTKNYTMMLAHENLRVVHVST
                     HVSLREACDRVKKQRVLDVIRIADKACKDLGIKEPKIGVAGLNPHSGEHGLFGREEID
                     EIIPAIEAAKAEGINADGPVPPDTVFSKARGGWYDIVVAMYHDQGHIPLKVVGFVYNH
                     EKKAWDAVAGVNITLGLPIIRVSVDHGTAFDQAGTGTASELSLRNSIEYAVMFANNKN
                     "
     gene            17893..18180
                     /gene="rpsF"
                     /locus_tag="ACZPCL_04255"
     CDS             17893..18180
                     /gene="rpsF"
                     /locus_tag="ACZPCL_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR1161731.1"
                     /translation="MNKYELTVVLNVKLEDEERAAAIEKVKGYITRFGGTVTNVDEWG
                     KKRLAYEIQKSHEAYYYFIQFESDSVCPNEVEAHVRIMEPVIRYLCVKVEA"
     gene            18228..18686
                     /locus_tag="ACZPCL_04260"
     CDS             18228..18686
                     /locus_tag="ACZPCL_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR1161732.1"
                     /translation="MNRVILMGRLTRDPEVRYSQGERSMAIARYTLAVDRRGRRGQDG
                     NDQTADFINCVAFDRAGEFAEKYFRQGMRVLVSGRLQTGSYVNKDGQRVYTTEVILDD
                     QEFADSKGAASDMSGYGQSAPSQRPAPTSAIGDGFMNIPDGVEDEGLPFN"
     gene            18740..19009
                     /gene="rpsR"
                     /locus_tag="ACZPCL_04265"
     CDS             18740..19009
                     /gene="rpsR"
                     /locus_tag="ACZPCL_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR1161733.1"
                     /translation="MAFNKTDRPEGAKMRRPGGMRRRKKVCVFCGDKNGVIDYKDTNK
                     LKRYVSERGKILPRRITGNCAKHQRALTVAIKRARHIALMPYVCE"
     gene            complement(19160..19324)
                     /locus_tag="ACZPCL_04270"
                     /pseudo
     CDS             complement(19160..19324)
                     /locus_tag="ACZPCL_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151144754.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5726 a         3778 c         4893 g         4927 t
ORIGIN      
        1 tgcaggccag gaacgttatg aatgtcgtaa aaatctggta cagtctttag tatctgcaaa
       61 ctacatcggc atccttgtat gggccgttct tatcggtatc gcacttcgta aggcatctga
      121 taccaccaag cagtctttaa atgatatttc cgcagctctt tcaaagaccg taacctggat
      181 catccagtgt gctccatttg gtattatggg tcttgtatac aataccgttt ctgtaaatgg
      241 actttcaact ttcacagaat atggtaaact gttagcagtg cttttaggct ctatgctgtt
      301 tatctacttt gtcaccaacc cgatcctggt attctggtgc atccgtaaga acccatatcc
      361 gctgatcttc aagtgcttaa aaaagagtgc cattactgca ttctttaccc gcagttctgc
      421 tgcaaacatt cctattaata tggaagcctg cgcagaaatg ggactggata aggatacata
      481 ttctgttacc attccactgg gagcaaccat caacatggac ggtgcagcga tcactatcac
      541 agtcatgaca ctggctgcag cccacacctt aggtatcgca gttgatatcc caagtgccat
      601 tatcttaagt atcctggcaa ctttatctgc ctgcggcgca tctggtgtag caggaggttc
      661 cctgcttctg atccctatgg catgctcact tttcggtatt tccaatgaca ttgccatgca
      721 gatcgtaggt atcggcttta tcatcggcgt gatccaggac tctgtagaaa cagcattaaa
      781 ctcttcttcc gacctgcttc tttctgcatc tgcagaattc agacagtggc gtctggaagg
      841 aaaagaaatc aagttttaat ttttgatcag ttttgattta gaaagatgag gatttatcca
      901 tgctcagcac cattgaaacg attaaccagg ttgtgaatga ttttatctgg ggtgttcctg
      961 caatggtctg catcatcggc gttggctttt atttaagctg ccgtaccggt tttttgcaga
     1021 tccgcaagtt ctcctatgcc atccagacta ccatcggaag gatgttccgc aagagaaatg
     1081 cttctgacgg agcgctgaca ccatttcagg cggtgtgtac ggctctggcg gccacagttg
     1141 gaacgggaaa tattgccggt gtagcaggag ccattgccat tggaggacca ggcgctgtgt
     1201 tctggatgtg ggtttctgcc gttcttggta tgtgtaccaa atttgcagag gtaactctgg
     1261 ctgtctacta ccgtgagaca aacaaagaag gggatctggt aggcggtcct atgtactata
     1321 ttaaaaatgg ccttggaaag aactggcagt ttttagctgt cctgttttcc gccttcggtg
     1381 tgctcactgt atttggtaca ggaaatgcca cccaggtcaa tacgatcaca acggctgtga
     1441 actctgctct cttaagctgc ggactgatct cagaaggggc agtaaagact tccaacctga
     1501 tccagggtat catcattgcc gctttggtag ccctgatcct gttaggcggc gtaaagcgta
     1561 ttggtcaggt aacagagaag ctggttcctt ttatggcgct cctttatatt gtgttatctg
     1621 taggtatcat cctctttagg atccaggcac ttccatctgt gatccaggcc atttttgaag
     1681 gtgcttttaa acctgcagct gtaacaggcg gagcagtggg aagtctgttt atgagtatga
     1741 aaaaaggcgt ttccagaggt atcttctcca atgaggcagg tcttggtacc ggctctatcg
     1801 cccatgcatg tgcagatacc agaaaaccgg taaagcaggg tatgttcggt atttttgaag
     1861 tatttacaga taccattgtg atctgtacat taacagctct tgttatcctt tgcagccagg
     1921 taccggtagg ttacggacag gcagcaggcg cagagctgac tattcaggga tttatttctg
     1981 tttatggaaa ctgggtatct gtctttactg cagttgccat gtgctgtttc gcattctcta
     2041 ccattctggg ctggggactt tacggcgcaa gatgtattga atttttattc tcagaaaaag
     2101 tcattaagcc gtttatggta gcatattccc tggtggctat tttaggggct actgcagatc
     2161 tgggtctgat gtggaatatt gcagagacat ttaacggcct tatggcgatc cccaacctga
     2221 tcgccctgtt ccttctttcc ggaacagtgg tgaaactgac aaaagaatat tttgcaggag
     2281 aaggcgctgg aaaataacca gagtatatag ccggggcagc tgttaaagcc cgcggttata
     2341 ccagatatgc atgaattgca tagcagatga aacaaatttt cattgatttt cagaaataat
     2401 gagactataa tgagcaacaa ggtaagtaca tatttaaaat gatgaaaatc agtaagaaga
     2461 attaagaaac ggaggataca agatcgtgga aaagaaaaat cttgactggg caaatatcgg
     2521 tttcagctac atgccaactg atatgcgtta tgtatcaaac tataaagacg gagcatggga
     2581 tgagggaact ctgaccgctg actccaatgt tactattaac gagtgcgcag gtatcttaca
     2641 gtactgccag gaatgtttcg agggcttaaa ggcttacaca acagaagacg gcagcatcgt
     2701 tactttccgt cctgacatga acgcagagcg tatgatggac tccgcatcca gactggaaat
     2761 gccagtattc cctaaggaga aattcttaga cgcattagat caggttgtta aggcaaacgc
     2821 agcatgggtt ccaccttacg gaagcggcgc aaccttatat atccgtccat atatgtttgc
     2881 ttcaggtcct gtaatcggtg ttaagccatc ccacgaatat cagttcagaa tgttcgttac
     2941 accagttgga ccatacttca agggcggcgc taagccgatc accatctgcg tcagtgactt
     3001 cgaccgtgca gcacctcacg gaacaggcca tgtaaaggct ggtctgaact atgcaatgag
     3061 ccttcatgca catgaagtag cacatgcaaa tggatttgat gagaacatgt tcctggatcc
     3121 ggcaaccaga acttatgtag aagagaccgg tggtgcaaac ttcctgttcg taaccaagga
     3181 cggcgaagtt gttactccaa agtctgactc tatccttcct tctatcacaa gacgttctct
     3241 ggtatacgtt gcagagcact accttggatt aaaggttacc cagagacctg ttaagctgtc
     3301 tgagttaggt gacttcgctg agtgcggact ttgcggtact gcagcagtta tctccccagt
     3361 aggcaaggtt gtagaccacg gcaaagagat ctgcttccca agcggtatgg atgagatggg
     3421 accagtgatc aagaagctgt atgatacttt aaccggtatc cagatgggcc gtatcgaagc
     3481 tccagaaggc tggatcagaa agatctgcta ataaatatca aacagacaaa catagaaatg
     3541 aaatttcctg gatatattgt tgtaacagta tatccgggag atttttttga tgaaaaacgg
     3601 aaagtgttta ttatgtacag tctgcgccgg gatcgtggca ctttgcctgc ttttatccgg
     3661 ctgcagtatt ctgggcaaaa aagaggaaaa ggtgcgggac ctcacgatca cgcctgtttc
     3721 tgacagtgga cttcctcagg agctgcagac gctgatcaaa cagaaaacat cagcaccctt
     3781 taaaatgacc tatacaacag atgaaaatct gtacatagct gttggatatg ggccccagga
     3841 aggcggcgga tacagcatct gtgtgaatga gctgtatctg acgggaaatt ctgttgtaat
     3901 agcaacggaa ttaaaaggac ctgaaaatgg agaggataaa ggcgttgaga gctccagtcc
     3961 atatattgtg gtgaaaacgg aattactgga tctgccggtt gtgtttcaat aaaaatgcat
     4021 gaaaaataaa aatgcgctct ttttttgaaa aataacatat gatataatga gccctaaaga
     4081 aaaacaagcg gctgcgcagc agacatttgt tttgtttggg ccattaacga atggaccgtc
     4141 tgcgtaagca gacagtagag cacgtcagtg cgtgtccatg agtgacgcat tgcgaataca
     4201 ccatgaggga gccgtattat gatatttaaa gacatttggt tagacatcaa agcagtacag
     4261 gaacgtgacc cggcagcaag aagtgctttg gaagttttac ttttgtatca ggggatacat
     4321 gctctgatct ggcatcgttt tgcccactgg ttttataagc acaaaatgtt ctttattgcc
     4381 agactgatct cacagatcgc cagatttttc actctgatcg agatccatcc gggagcgcag
     4441 ttaggacatg ggatcctgat cgaccatggt tccggcgtgg tcatcggtga aaccgctgtt
     4501 gtaggtgata actgtacgat ttatcagggc gttacattag gcggtgttgg tacccagaaa
     4561 ggcaaacgtc atcctactct tggaaataat gtaatggtag gtgccggagc caagatatta
     4621 ggagcctttg aagtaggtga caactgctcc attgctgcca atgcagttct tttaaaacca
     4681 ctggaagata atgtaacagc agtgggaatt cctgcaagac ctgtaaaaaa agatggtgtt
     4741 acacttccaa aacggaaaga gaccatgact ctggaagaat atgaaacaat gaaagagcgt
     4801 atgctccaga tggaagagga gatacgccag ttaaaagaaa agttaaataa gcagtaaagt
     4861 tataaaacac ctgttgtccg catagacttt cgtaaaagac tattagaacg gcaggtgttt
     4921 tttatggaat taataaagaa gcagatacat atgaaccggt ggaaaggaaa tgcagtgacc
     4981 cagatcactc tggatgaaga ttttattgtt cctgatacac tggaagatat ggaacaggtc
     5041 atactggagc aggctcatat ccagggggaa aatgtaaaga cccaggagga gaagataacc
     5101 gtaaaaggaa aactggaatt tcaggttctt tacagaaaag aaaatggggg actggaaaca
     5161 ttaggaggaa acattccttt tgaagagact gtaaatgttc cgggactggc agaaggagat
     5221 catacaggtt taaactggat cctggaagac atgaaaacgg atatgatcaa ttccaggaag
     5281 ctgggggtaa aagctgtgat cacggtggag atccatacag agggagaaac atcccagatg
     5341 gcagcttcgg acctgaagga gacgggaaca gccggaagtt tttcaagtca gacagaagaa
     5401 atgcccccgg aagtgaaaat gggaagtatt tctcctgtta tactggctct tcaccgtaaa
     5461 gatacttacc gtatacggga aaatacggtc cttacagggg gaagaccgga tatagaaaat
     5521 ctattgtgga aggatatttc cataagcagc cagtctgtaa agccgttaga tggccgcctg
     5581 cacctggaag gacagcttac attatttgtg atctatgaaa cagaagaaga aaatatgccg
     5641 gtgcagtgga tggaagaggt gatcccattt tcaggtgata tagagatagc ccaggcacat
     5701 gaaggacaga tccccatggt atctttgcgc ctggctcatg gagaagtgga ggcgaaaccg
     5761 gatccggacg ggcagatgcg ggaactggat ctggatgtag tcttagatct ggatatccgg
     5821 ctgtatgaag aaaaagaaga aatgctgtta aaagatattt attctaacag ctgtcagatc
     5881 acgccggaga aaaatgagat gggatatgac cggctgatcg caagaaatgt atgcagaccg
     5941 aggattgggg agaaaataca tctggggcag caggagcgga tccttcagat ctgccatacc
     6001 cagggaatgg tacagttaga tgagatcgaa ccggaagaaa acagtttgga tatagaagga
     6061 atcctggatg tgaccattct ctatctcacc agtgatgaca gtgccccaat ccagtctgtt
     6121 tccaggcaga tcccttttaa atgcagcgcc caggcagcag gtattaccaa aaagagtatt
     6181 tatcagctgg atgcaggagt agatcagctg acagctgtaa tgacgggagc agatacggtg
     6241 gaagtaaaag gtgtggcagc cctggacttt atgatcctgg aaccggtaca ggaaacggtg
     6301 ataaccggaa taaaggaaga acccctggat ctgaaacagt tacaggaact tcccgggatc
     6361 gtcggatata ttgttcagga gaaagactct ctttgggata ttgcaaaaaa atttcatact
     6421 acagtggaaa atatcgttac caccaatgaa cttccaggtg aacaggtaaa aacggggcag
     6481 aggctgcttc tggtgaaaga ggtgggagtg taggtggacc gcctgtgcta tggagaaaaa
     6541 agtaatgtaa gatagatagg aaatatggta taatctgatg tgcgcaaacg aaaaaagaaa
     6601 tgaggtacga aatggatttg aattttaggc ctgtagaggc tgaagatatg tccaggctgc
     6661 gcccttttta tggtctcagg cccaacagga cctgtgacag cgtattcctg gactcttttt
     6721 tatggaaaga ttactacaag gtagaatgtg cagtcagcga agacaaggca gttttatggc
     6781 ttatggaaaa agatggcata ctttcaagcg ccatgcctct ttgcaaggaa gaagatcttc
     6841 catatttctt ctatcagatg gtagattatt ttgacaatgt actccataaa cctttctata
     6901 tttctctggc tgatgcagaa gctgtagaat atctgaacct ggatccggaa aagtttgaag
     6961 taacagaatt agatgatctg aaagattatc tttatgatgg aaatgccatg cgtacactgg
     7021 ctggaaagaa gcttcataag aagaaaaacc gcttaaacgg ctttttaaag acctatgaag
     7081 gcagatatga ataccgcagg atctgctgct ccgaccgtca ggaagtatgg cagtttatgg
     7141 aaaaatggag acagcagaaa gtagagtctt atgaaaatga gatctccctg gattacgagg
     7201 tagccggtat ccatgatatt ttaaagaact gctctgcttt caatgtgccg atggcaggcg
     7261 tttacattga cggggaattg aaggcattta cagtaggaag cttaaatgag cgggaaaata
     7321 tggcagtgat ccatattgaa aaggctgatc cggagatcaa cggtctgtat cagtttatca
     7381 atcagcagtt tttagttcat gaattcccgg atgtagccct ggtaaacaga gaagatgatg
     7441 taggtatgcc gggactgcgc aaggcgaaga tgagctatta tccggtggat tttgcaagaa
     7501 aatattcggt taagaagaaa tactaagatc atgaaaatga ggatgaaata ttccattaaa
     7561 tgaggctgtg atactgggca gaaagaggcg gatatggtac gatatttaga gaaaagtgaa
     7621 tatggaaaat gcaggccctt atgggaagag gcatttccgg aagactcaaa aagctttgca
     7681 gattactatt tttccgaaaa acttcccggc agtaaagtgc ttgccataga aggaggaaaa
     7741 cttctgacca tggctcactt aaatccctat accatttctg taaatggaaa aaaatgggaa
     7801 ctgccttata tcgttggtgt agccacagct gcccaccagc gccataaagg ctatatgcgc
     7861 caggttctta atcaaatgct ccgggactta aatcaggaga aaaagccgtt ctgcttcctg
     7921 atgcctgctg cagaggctat ctaccggcca tttcagtttg cttttatcta tgaccagccg
     7981 gtatggaagc ctgaggatga accggaaaaa gatctggaaa aggttcctgt agatctggcg
     8041 gaaaagtcag aagagctggc ttactggctg aataactggc ttgagaaacg ttatgaagtc
     8101 tatgcagtga gagacagggc ttatatggaa cttcttaaaa aagagctgga aagtgaagca
     8161 ggagaagtga cggggctgta tgaaaaagac ggaaaacttc atgctttgga agcctggtgg
     8221 ggacttggaa aaagagaaga gcggttttat tacagcatat cggaaataaa gccttcagat
     8281 atgcacccgg cgatcatggt ccggatcaca gatgtaagat cattgttaga agtcattgga
     8341 ttaaatgaaa acgcgccagg agataaattt caggcagttc tttccataaa agatccgatt
     8401 atttcggaaa atgaaggatg ctggttgtgg aaactgggaa aaaatggaag tactctggaa
     8461 cggatgaagg gactggggct gcagacagag gcagatgagg cagaacagat cccatcttcc
     8521 agcgaagtgt tggagatcaa cattgacgag ttggcacagt ggatctttgg gtacaagacg
     8581 ctggaagaga cattaggtgt gatgccaccg ttctggacag aatatgtgag gacgctgcaa
     8641 ggggtatttt tggacgaggt tgtgtaggag gaaacgcata tggatgaaaa atggcagcag
     8701 ttaaaagact ggatcaatga aagcgacaac atcgtattct ttggcggagc cggtgtttct
     8761 acggaaagcg gcataccgga tttccgcagt gtggacggac tgtataatca gaaatataag
     8821 tacccgccgg agcggatcat cagtcacagc ttttacatac aaaatccgga agagttctac
     8881 cgcttctata aagaccgtat gctgtttccg gatgcaaaac ctaatgcagc ccataaagcc
     8941 cttgcaaaac tggaacagga aggaaaatta aaggctgtga tcacccagaa cattgacggc
     9001 cttcatcaga tggcaggaag caaagaagtg ctggagcttc acggttctgt acacagaaat
     9061 tattgcaccc gctgcggcca gttttatgat ctggactatg tgagaaacag cgaaggcgtt
     9121 cctacctgca cctgcggcgg ccgtatcaag ccggatgtag tcctttatga ggaaggactg
     9181 gatgaccgga ctatccagaa gtccattgat tatatccgca acgcggacat gcttattatc
     9241 ggaggtacgt cccttgtggt ctatccggca gccggtctta ttgactatta cagaggcaac
     9301 aagctggttc tgatcaataa aagcgtaaca tccagggact cccaggcaga tctggtgatt
     9361 tctgacagca tcggaaaggt tttgggaaca gttgttggaa atgacaatgg atcaggaaga
     9421 tccaaggaga aatagaacta tgccaggaga acgtttaaac tacgaagtag gagatatcgt
     9481 aaaattaaaa aagccccatc catgcggaag cagtgaatgg gagatcttaa gagtaggagc
     9541 ggatttccgc ttaaaatgta ccggctgcgg gcaccagatc atggtagccc gcagactggt
     9601 ggagaagaat acaaaagggt taaagaaaaa agacgagatc accggttgat atagcgccac
     9661 aaagtggtcc ggctgattcc cagcttcttt gctgcagctg tctggtttcc attgcagctg
     9721 tttaaaacat gctggacaat gtcctggttg atgcggtcaa gaggctgttc taagttcagg
     9781 gaaaagcctt ctttttcggt ggctgctttg gaatgtgtta tcatatgcgc ttcatggtca
     9841 tccgggaaaa aggcagattc ttcttttaat attttttcaa tggtatcacg ggatatatag
     9901 gcagtgctgg tttccagtac tgcttttttt aatatacgtt taaactgggt gcggttacat
     9961 ggatagtcat attccataag aagctttgcg gcaccgtctt ccagtcctac cacctggcgg
    10021 cccagattct ggttcaacgt atcaatataa agagcggctg aaggaacaat gtcatttttc
    10081 tgttcccgta aaggcttgat aggaagaggc acacatccaa gcatattggt atactccaat
    10141 gcagcatggg gcatatgacc gtcctttgcc tgggtgcagg aaaagatcag acggttgcgg
    10201 atatgcaggt tggtgtccag tatgatagat aacagctgtt tctgccgcgg gtgggacaaa
    10261 acgcccagat tggagatgta aatggtattt ccgttgtctg taaaaggaga gttgtaatga
    10321 ttgatcagga aattccaggt tttatcattt aacatggaac agtttacaac gtaaagcggt
    10381 tcatcattaa actggctctt tgcatagtgg agataagcta cacggtcttt gcctgtaccg
    10441 atctctccgg tgatcataag agggactgaa gaagaacctg actgatccat ggcagcggca
    10501 gcgctgcggg atagttctgt attgctgtaa aagctttcaa taaagctttg taatgcctgt
    10561 tctttatcca taatggtgat gccgtattta gagtggctta aagggatctt ggataacatg
    10621 atgcggaaga tgatataagg gaaatcccct tcctcagcca ggctggagcg gacggaatac
    10681 agctggtttc ccagtgtgac aaagaaagaa cgcctggagg aagtgcggca ttttccaagc
    10741 tctttttgca ggctctggat aaattcttcc tctttttcat cattgattgt ggaataatgg
    10801 cagcggcctt ccagatccag gatcagatac tggttggagc tgctgcggat cagggactgc
    10861 atcatggcat tggaattgca cagcttctgg tactgctgcc aggtgccaat ggcattttcc
    10921 atggcctgtt ttacgctttc tgcgctggag gtaagaagga taggcgtaag tccgatcatt
    10981 tttgcatggt cataagggat catatcgcag actacagttt tatagccctg ttccttcata
    11041 cggtccagaa gatcgccgga gcctttccag ttatctacgg agaaggagaa aatatctaca
    11101 tggatctgga gaagatcgca gaggtttttt gcgatgacag tcaaagactg aaagcctaaa
    11161 agggcaaatt tggtcccggt attctgggcc attttaatac agcgcagtac atcataatag
    11221 ccaataccaa tatcgatgac cgggatatgc acagactggg agatcatctt ggctgtgttt
    11281 gcgcgggaga tgataacgtc ataatgttcg ctgtagcgtt ttgcaagggc aagtccctgt
    11341 tccatgcttc ctgcataggc ggtaaattcg atctcagggt attgcagggc acattgtttc
    11401 ataagagtgg caagtccttt atagggagca attcccagta tacggatttt ttttatcatt
    11461 taagtcacct cacatttata aatgttacaa atttaaacat attatacaat aaattcattg
    11521 aaatagaaag acaaaagcgt gtagaatata aaacgaatga gaaaaaaatc taaaaaaaat
    11581 atgaactctc acaaaagtgt ttcaaaaatg aacaattaga aaggcggtga aatggttgga
    11641 agaaagatac ctgatcattg cagatgattt taccggagcc aatgataccg gagtccagct
    11701 gcgcagaaga ggttttccaa cagaagtgct gttttgcgga aagccgatgg gtgcagacag
    11761 atccattgtc attgatacag agtccagaac agttcatcct gatcatgcat atgagatagt
    11821 gtcccatgcg ctggaagatg tggattttga cagcttccgc tatgtgatca aaaaagttga
    11881 ttccaccatg agaggaaata ttgcagcaga gatcaaggca gtagatgaac atttcaaacc
    11941 ggaattaatg atctttgcac cggctcttcc ggatttaaag agaacgactg tagatggggt
    12001 gcagtgttta aacggcgtag agatctgccg cacggagctt gccagtgacc ctaagaaccc
    12061 ggtagtagaa gataatctgg tacggatgct gggaagatat tatgaagaaa aggtcatttt
    12121 aaagagactt ccggatgtga gaagtgatga tcttgacttt acagaaggcc gcatttatgt
    12181 atgcgatgca gactgcaatg atgacttaaa gaaagtttta aaggcagttg caaagacagg
    12241 aaagaaggta ctttatattg gtaccgcagg tctggcagat aacctgatgg aactggaacg
    12301 tccgtctctt ccggccctgg gagtggttgc aagtgtaagc actgtgacta accggcagat
    12361 gaaatactgc gaagaagccg ggattaaaat ggtaaagctt cctatgcatg atattctgtc
    12421 cggaaaagag gatacagagt cttatctgaa agaagtggta gattctttaa aggcaggtaa
    12481 agataccatt cttcttacta atacagccta tgacagaagc gaactggaac tttcctttga
    12541 agctggtgag gcgttaggac ttggccctgt agagaccggt gataaggtac gttccatagt
    12601 tggccgtctg gcaatggaga tcctggaaca ggcaaaagtt tccggcgtat tccttacagg
    12661 cggcgataca gcccttggaa tgctgatgaa cattgaagca gacggttcac agatcctttc
    12721 cgagatccgc gtaggtatcc cgttagtccg ggtaaaaggc ggaaagtacg aaggaatgaa
    12781 actggtaaca aaggcaggtg cttttggagc tgatgatgca gctgcatttg cattgcgtaa
    12841 gatcaaagaa gcataaaaac aacttaaaac ttaacgatcc aatcattcat ttaaaacagg
    12901 aggaagcaga gatgaaagaa tttttattac cttatggaaa agaaaaatta acagcaaaga
    12961 tcgaggatga gcatctggca ggtgtgctgt tatctgaact tcacagctac aaggcgccaa
    13021 agagcggggc agagctggtt caggatgctt tggagcatcc aatcggaact ccaagactgt
    13081 gcgatatggc agttggaaag aaaaaggtag ttgttatttc ttctgaccat acaagacctg
    13141 ttccaagcca catcatgatg cctctgatct tagcagagat ccgtaagggc aatcctgatg
    13201 ctgatattac cattcttatt tcaaccggtc ttcacagaac aactaccaaa gaagagctgg
    13261 cagcaaaatt tggtcctgag atcatggcaa atgagaagat cgtagttcat gattgcgatg
    13321 acaaggacaa cctggtatac cttggcaagc ttccatccgg cggcaatctg atcattaacc
    13381 gtctggcagc agaagcagac ctgttagtag cagaaggctt tattgagcca cacttcttcg
    13441 caggtttctc cggcggcaga aagagtattc ttcctggtgt tgcaagccgt gagactgtta
    13501 tgtacaacca taactccgga tttattgctg atcctcatgc acgtaccggt gttgttgaag
    13561 gaaacccgat ccacaatgat atgctgtatg cagcaagagc agcaagactg gatttcatcg
    13621 taaatgtagt tattgatgca gcacatgatc ctatttttgc agtagctggt gactgtgatc
    13681 tggctcacag agttggacgt gagttcttag cttccaagtg ccaggtagat gcaattccgg
    13741 cagatatcgt tatctccacc aacggcggat atcctctgga ccagaacatt tatcagtctg
    13801 taaagggtat gaccgcagct gaggcaactg taaaggaagg cggcgttatc atcatgcttt
    13861 ccaaggcagc tgatggacac ggcggaaaat acttccatga gactttccgt gatgagaagg
    13921 atctgaaccg tatgatgaag accttccttg acagaaaacc ggaagagacc attatcgacc
    13981 agtggcagtc ccagatcttt gcaagactgc tgttaaaggc aaccgtagta tttgtatctt
    14041 cctgcgatga ccagttagta gaagacctgc atatggttcc tgcacacagc atggaagaag
    14101 ctttggagaa ggcaaaagcc atcgttaaga aggatgacta caaggtaact gttatcccgg
    14161 atggtgtatc tgttatcgtc agagagtaat tgatcaactt tgaactctga attgtctgga
    14221 agcaggcttt ctggtgtgtt tccagacaga tgaaataaat gtactcccgg agtctttctc
    14281 gcaagatcag gtacagaaag attccgagag aacatataaa gaaaaaggag aaagacagac
    14341 atgaaatttg ttctcattcc tgattcattt aagggaacct taagttctga gcagatctgc
    14401 gagattgcaa aggaaaaagt agaaaaacat tttcctggag ctgaaacagc ctgcgttcct
    14461 gtagcagacg gcggcgaagg ctctgtagat gcatttatta ccgctttagg cggcagcaag
    14521 gtaactgtaa aggttaaaaa cccatatttt gaagatatgg actcctacta cggtctgatc
    14581 aacgaaggca agacagcagt cattgaaatg gcttcctgcg caggacttcc attagtagaa
    14641 gaccgcaagg acccaagaaa gaccactaca tatggcgttg gacagctgat cttagcagca
    14701 gctgaaagcg gcgtagaaaa gatcatcgta ggactgggcg gatccagtac taatgacggt
    14761 ggttgcggtg cagcagcagc agttggcgtt aaattctatg acaaagacgg aaaggaattc
    14821 gttccaaccg gcggaaccac cgcagatatc tgtaagatcg acttaagcgg caaggcagag
    14881 atcttaaata aagttgagat cgttaccatg tgtgatattg ataacccaat gtacggacca
    14941 accggcgcat cccacatctt cggacctcag aagggtgcag atgaggcaat ggtactggaa
    15001 ctggatgaag gtatcaagaa cttaagccgt gctattacag aagccatcgg caaggatatc
    15061 agtgaagttc ctggaaccgg tgcagcaggc gctatgggcg caggcatgat cgccttcttt
    15121 ggctcccgtc tgcagatggg tatccagacg gttctggaca ctgtaaagtt tgacgaaatg
    15181 atcagcgatg ctgattacat tatcaccggt gaaggaaagc tggattccca gagtcttcgc
    15241 ggcaaggttg tgatcggtat tgcagagcgt gcaaagaaac agaataaaaa tgtgatcgct
    15301 gttgtaggcg gtgcagatga tgcagagatc gataaagcat atgaaatggg tgtaactgcc
    15361 gtattcccta tcaaccgtct gcctcaggat ttctccataa gccgtcatca cagcaaggag
    15421 aacctggctt atacagtaga taacgtttta agactgatcg ctgcaaaata aaaaatataa
    15481 tttaacataa gggggatact agaatgaaca ttctgaaaac cgtgaaaaaa attcctggtg
    15541 gtttaatggt agttccgttg ctgttaggtg caattattaa tacatttttt ggcggtacga
    15601 tctggaccat gtttgatggt acatttacta catacctttg gaaatcaggt gcaatgccga
    15661 tcctggcagt gttcattttc tgtaatgcaa caactatcaa cttcaaaaaa gcaggcgtta
    15721 ccgtttataa aggcgttgta attaccgctg tcaaagttgg tgttggtatt atcattggtc
    15781 tgatctgtgg taagctgttt ggcgaagcag gtttcctggg actgtccaca ctggcgatcg
    15841 taggtgccct ggcaaactcc aatggtggtc tgtacgcagc attagctggt gagtacggtg
    15901 atgcaacaga cgtaggtgct gtttctatcc tttctatcaa cgatggtcct ttcttcacca
    15961 tggtagcttt aggtgctgct ggtgttgcaa agatcccaat gtccgtactt gtaggctgta
    16021 tcattcctgt aatcgttggt tgtatcatgg gtaacttaga tgaagacatc cgtaagttct
    16081 gcgaaccagg tgcttctatg ctgatcccgt tcttcgcatt ccctctgggc gctggcttaa
    16141 acatcatgaa cctgatcaag gcaggtggcc ctggaatcct tttaggtgtt ggctgtactc
    16201 ttattacagg tctggttgga taccttgtat acaagctgtt aagaatggat catccggaag
    16261 ttggtggtgc tattggtaca acagcaggaa acgcagcagc aactcctgca tctgtagcag
    16321 ctgtagatgc aactttactg gcagtatctg aagcagcaac tgctcagatc acagcagcta
    16381 tcatcgtaac cgctatctgc tgcccgatcc ttgtatcatg gctccataag cttgaggata
    16441 agcgcagagc agcaaaataa gtaaatagat aataaaagaa taagtacagc tggtttggct
    16501 gtaagataag tgggaccggg tggaagcatc cggtcctgtt tgttacagaa atgccgaccg
    16561 cctgcgtcag cgggcgtgtc tgagtaggaa atctaagcat atgacatact attttcaata
    16621 aagtatggta tattgaatac agaaaatttt attattccat ttttcaggag gcagatatga
    16681 gcgaaaagaa gatcgtaggt attaccatgg gagatccggc cagtatcgga ccggagatta
    16741 ctgtaaaagc atttgcagac aagtccttat atgacctgtg taacccggta gtagtagggg
    16801 atgcctgcgt aatggaagca gcacttccta ttgtaggaca tacagaaatg aagatccatg
    16861 ccattaagga tgtttctgaa gcaaaatatg aatatggaac cattgatgtg ctggatatgg
    16921 gtctggttga catggcacag ttaaaaagag gggaagtttc cgctatgtgc ggagatgccg
    16981 cttttaaata cgtaacaaag gttattgaac tggcaatgga taagaaggta gatgctactg
    17041 ttaccaatgc ttttaataaa gaagctgtaa acctggcagg acatcattat tccggacata
    17101 cagagatcta cgcagatatg accggcacca agaactatac catgatgctg gcacatgaaa
    17161 atctgcgagt agtccatgtt tccacccatg tttccctgcg tgaagcctgc gaccgtgtaa
    17221 agaagcagag agtattagac gtgatccgta ttgcagacaa ggcatgcaag gatctgggca
    17281 tcaaggagcc aaagatcggc gtagcaggcc tgaacccaca cagtggtgag cacggcctgt
    17341 ttggaagaga agagatcgat gagatcatcc ctgcaattga ggcagcaaag gcagaaggca
    17401 tcaatgcaga cggaccggta ccgcctgata ctgtattctc caaagcaaga ggcggctggt
    17461 atgacatcgt agttgccatg tatcacgacc agggccatat cccgttaaag gtagtaggtt
    17521 ttgtatacaa ccatgaaaag aaagcatggg atgcagtggc aggtgtaaat atcactttag
    17581 gactgccgat catccgcgta tccgtagacc acggaactgc atttgaccag gccggaaccg
    17641 gtacagcaag tgaattaagc ctcagaaatt ctattgaata tgcggtaatg tttgcgaata
    17701 ataaaaatta agtatgtgcc gttgacaaat ggcggccgtt atcgcaaaaa agtccttaaa
    17761 aagtcttgaa aaaattgcaa aaaggtcttg catttaaagg ctttctttgc tagaataact
    17821 attcgtgaca tataaaatcc ttgctcctga aactgaaagc aggggccaga gaccacaagg
    17881 aggtaaaaca agatgaacaa gtacgaatta accgttgttc tgaatgtgaa gcttgaagac
    17941 gaagagcgtg cagcagcaat cgaaaaggta aaaggttaca tcacccgttt cggtggcact
    18001 gtgaccaacg tagatgagtg gggtaagaag agacttgctt atgagatcca gaagtctcat
    18061 gaagcatact actacttcat ccagtttgag tccgattctg tatgccctaa cgaagtagaa
    18121 gctcacgttc gtattatgga accagtgatc agatacttat gcgttaaggt tgaggcataa
    18181 gaatacccag accgtgaaaa cggagttaaa aaaggaagtg tgaccgtatg aacagagtta
    18241 tcttaatggg cagattaaca agagatccgg aagtaagata ttcacaggga gagcgttcta
    18301 tggcaatcgc cagatacact cttgcagtgg acagaagagg ccgcagaggt caggatggaa
    18361 atgaccagac tgcagatttt atcaactgtg tagcatttga ccgcgcaggt gagtttgcag
    18421 agaagtattt ccgtcagggc atgcgtgtac tggtttccgg aaggctccag acaggcagct
    18481 atgtaaataa agacggtcag cgtgtataca ccaccgaggt catccttgat gaccaggaat
    18541 tcgcagacag taagggtgcg gcatctgata tgagcggcta tggacagtca gctccatctc
    18601 agagaccggc tcctaccagc gcgattggtg atgggtttat gaacattccg gacggagttg
    18661 aagacgaagg acttccgttc aactaggctc tattatgccg gacatgttcc atggttaata
    18721 ataaaggagg acaacaccaa tggcatttaa taaaacagac agaccagagg gcgctaagat
    18781 gagaagacca ggcggaatgc gcagaagaaa aaaagtttgc gttttctgtg gcgataagaa
    18841 cggcgttatc gattacaagg acactaacaa attaaagaga tacgtatctg agagaggtaa
    18901 aatccttcct cgtcgtatca ctggcaactg cgcaaagcat cagagagctt taactgtagc
    18961 tatcaagcgc gctcgccaca tcgcactcat gccatatgta tgcgaataat atttgattaa
    19021 aatatgtaag tattttgaga ctctgcgtga gaaatcacgc agggtttttt tgtactacaa
    19081 taggaaagtt cgacttccgt cgaactcttg ataaaaagag cccgcacaaa ggcgggcatg
    19141 gcaaactgaa gcggtgtttc gatttgtgaa taccgctttc cgaaagaata cagtgaatat
    19201 gtggattcca ttcaagaggg cgaccgaaag tgtgaagcac acaaataaaa ccaggaacaa
    19261 agttttgaga cttatttatc ttattaaaca tgtgaaggat gacgctttga acagcacgaa
    19321 aaag
//
LOCUS       DBMDUA010000032        18937 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1754_length_18937_cov_3.991559, whole genome shotgun sequence.
ACCESSION   DBMDUA010000032 DBMDUA010000000
VERSION     DBMDUA010000032.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 18937)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18937)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18937
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1754_length_18937_cov_3.991559"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            152..739
                     /locus_tag="ACZPCL_04275"
     CDS             152..739
                     /locus_tag="ACZPCL_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1161734.1"
                     /translation="MDDTQRKITKIAREAAKFTVQTMKAEGIGTAEFDFIHLVRHKPG
                     ITQAEIRETLKIDKGAAARRAASLEAKGYLVRKPNPEDKRSQLLFATEKAEELKNSKA
                     SIEDAFYDWLIADLSEADRREFCRILDILYWKSKEERRVDFTHVSAWTNEKLSRIPSE
                     EMTDQKIPDKEILNKELPPKKAPEKEPQEGELSHE"
     gene            732..2513
                     /locus_tag="ACZPCL_04280"
     CDS             732..2513
                     /locus_tag="ACZPCL_04280"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1161735.1"
                     /translation="MSKQSAPPICKHPDHVTSYFSMQKGILAVITVSGLIYNLGLMAR
                     PWFEGQMIQCLFFIQKGQSHFSDMLKLASAYFIAIFTVQISRFIKRLYVRRFANNINR
                     SMKQTYYNNLVHTDTSDLKNVNTGNAMTKALSDVDACSEGIRKFTTEIFDTGVALFSY
                     VFMLFAYDWKLALLCLIFPPVSYVIAERMKVVVLKTGAACKESAGRLNAATLDRIQNA
                     ITYRIYGCETQRNKDYEAHLADYEKASVRADLPVAALPPIYKLISMTGVLLIFYFGSK
                     NVMGTGWTSWDIAAFSAFLSCFSRLSVKSSHAAKLFNAIQKAQVSWKRILPFMKAGKE
                     DKPLPASSLGQVNARSLGFSYENSAPLFKDLTFQASPGEIIGITGPVACGKSTLGRIF
                     LCEHPYTGSLTFNGKEFSSLSPDVRNSLVGYLGHEPDLLSDTIKNNVLLGDQKDLWKY
                     LEAVCLEDEVRHMPQGEDTVTGETGLRLSGGQQKRLALARTLAHPRPILILDDPFSAL
                     DKTTEVEVFEHLRQMVPDSIIFLISHRLYLFSETDGVIWMDNGTAEFSSHKKLLKDNS
                     AYRELYQLQTDYSVAKPTESAIERGIC"
     gene            2516..4159
                     /locus_tag="ACZPCL_04285"
     CDS             2516..4159
                     /locus_tag="ACZPCL_04285"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1161736.1"
                     /translation="MKTEHSVLKVILRTVKQNKGMCVTLFAAVLGSAVFALLPPLVLA
                     KAIDYLTAGQTFPFALALSYFGLLLLTNAATSLRESLLTIFGQRITHGLRTVLCQKIS
                     LLPADSFVSHDSGAIASRFVGDVDTVEELFTSGIISMFADACQLCGIFFVIFTKNRGL
                     AILLLFVLPAVFFYTRHVQKRMLASQLSNRAAIAKASGHVPETLRCIRTIHNLHKEMY
                     MEEKYSKFLEDSYKAIDKTNFYDAIYSPVILISNSITIAIVMILSAFGNGEVQLFFGM
                     SAGTAAAVISYITQVFTPLESIGMEIQTIQSAAAGVHRINEFLQLPERTKARETNTSR
                     PDPAACPVELQNVTFRYEESDPDILQSLSFSVKEGEQVTLTGRTGAGKSTIFKLLLGL
                     YTPQKGNVLINGQPADALSENQRRKIISCVEQNFSMVPGTILDQITLFDPDITLEQAQ
                     TAAGLTGLDEVIRSFPEGYNTPCKPGLFSQGQWQLLSIARAVATNPRILLLDEITANL
                     DADTEQTVLSALNRAGNGRTVLSISHRLYEKNGGRQIAI"
     gene            complement(4217..5563)
                     /locus_tag="ACZPCL_04290"
     CDS             complement(4217..5563)
                     /locus_tag="ACZPCL_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1161737.1"
                     /translation="MEHSKETGTDQELMRSMKVSKAVAAMAIPSVISSLVTVVYNMAD
                     TFFVGQTGDALQVAAVSLTNPIFILLMAFANMFGMGGSAVASMAMGEGKPKKVKNTTA
                     FITYASLAVGIIIAVILFAFMSPILHIFGANTETYEYAKGYIHYIAWGAPFIIWSAAA
                     SFVVRAEGASKEAMIGSMVGTIANIVLDPLFISGLHMGAAGAAIATTIGNILASLYFV
                     WYFLKRSGQLSILPKDFTLGENIPQRVCTTGFPTAIFSVLMSVSTIVVNQILVKYGNA
                     PVAAIGIVFKANMFITFLQMGLANGVQPLLGYSYGAGNISRFREVERFTKICCLIAGV
                     AATLLYFVGKEPIIRMFISDDEVVYYGVKMLIAYMLSGPVIGMLFVNMNCMQSVNYAL
                     PATILSVMRQGVLLIPLLYILQLIAGLEGVIYGQAITDYIAVVLSTVFWRNIRKKL"
     gene            complement(5643..6068)
                     /locus_tag="ACZPCL_04295"
     CDS             complement(5643..6068)
                     /locus_tag="ACZPCL_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1161738.1"
                     /translation="METGKVINRISNRLRRRSAALQETLGMSGAQGNILNFILVEGQK
                     HSIYQKDIEKEFGLRPSTATEVLKALEKKELICRVSEKQDARLKRIEFTSKAEEIRHL
                     IAAEIGESESILLKGITEEERQIFIEIGERMLKNLDEAN"
     gene            complement(6257..9085)
                     /gene="uvrA"
                     /locus_tag="ACZPCL_04300"
     CDS             complement(6257..9085)
                     /gene="uvrA"
                     /locus_tag="ACZPCL_04300"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR1161739.1"
                     /translation="MPKQYIKIRGANEHNLKNISVDIPRNELVVLTGLSGSGKSSLAF
                     DTIYAEGQRRYMESLSSYARQFLGQMEKPEVESIEGLSPAISIDQKSTNRNPRSTVGT
                     VTEIYDYMRLLYARIGIPHCPKCGREIHKQTIDQMVDQIMAMPERTKIQLLAPVVRGR
                     KGEHVKVLEQARKSGYMRVRIDGNLYELSEEIKLEKNIKHNIDIIVDRLVVKAGIENR
                     LTDSIETVMSLSNGLMTVDVIGGEPVNFSQSFSCPDCGISIDEVEPRSFSFNNPFGAC
                     PECFGLGYKMEFDEDLMIPDKSLSISQGAIVVMGWQSCTDKGSFTRAILDALAEKYHF
                     SLDTPFQDYPKEIHDIIIYGTGGHEVKVRYKSQRGEGIYDVAFEGLIENVNRRYKETF
                     SEASKAEYETYMRITPCPACKGQRLKKESLAVTVGGLNIYQATNMSIVKFKEFMDGLT
                     LTPMQQTIGASILKEIKARISFLIDVGLDYLSLSRATGTLSGGEAQRIRLATQIGSGL
                     VGVAYILDEPSIGLHQRDNDKLLKTLLHLRDLGNSVLVVEHDEDTMRAADCIVDIGPG
                     AGEHGGQIVAVGTAEEIMKNPESITGAYLSGRLQIPVPDQRRTPTGWITVRGAEENNL
                     KKIDVSFPLGVMTCVTGVSGSGKSSLVNEILYKALAKKLNRARTIPGKHKCIEGVEQL
                     DKIIAIDQSPIGRTPRSNPATYTGVFDLIRDLFASTADAKAKGYNKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVPCEVCGGKRYNRETLEVKYKGKSIYDVLDMTVEDALK
                     FFENVPSITRKIQTLYDVGLGYVRLGQPSTELSGGEAQRIKLATELSKRSTGKTIYIL
                     DEPTTGLHFADVHKLIEILRRLSDGGNTVVVIEHNLDVIKTADYIIDIGPEGGDKGGT
                     VIAKGTPEEVAESPVSYTGKYVKKYLEQ"
     gene            complement(9217..10962)
                     /locus_tag="ACZPCL_04305"
     CDS             complement(9217..10962)
                     /locus_tag="ACZPCL_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1161740.1"
                     /translation="MRELIERYVRELVKIPSTSNTETEKSCADYIAEELAKQPYFKEY
                     PEQTGKYLLPEDSFGRSVPWGLVKGRNGSRKTIILTGHYDVVDTEEYGNERALAYDVE
                     KWEDLVKSGNALPGMPEEVKKDFLSGDWMFGRGTADMKGGLSVGLALLDWYGKLVVEA
                     ERKECGAAVFETKTASGTEETPEISGNLLFVTVPDEEGYSAGMRDAVPFLNDLKERFD
                     LEYTALIDLEPASMENGAKTIYTGSVGKTMPAVLVQGVKAHVLNCFQGVSSVGVLSSF
                     FMKTELAPEFAEKSATEICPPPTWFCLRDRKEGYDVSVPFRAGGYMSILGFEKTPDEV
                     IKRLKELGKESFEEYARRIEAQWKAVEKAEVPEEKAGLTSEGNPLACPGTAAAAEAEV
                     LTVSELLAYCRKEQGEAFTAWLSEAYKTQKARLDKGETNFPSATLDFMEQLLNQSGIS
                     GPVMILGFAPPFYPAVDSGKEGKILFKEMKKAAEKENVSLKCHEYFCGISDLSYCGGM
                     DKEELAAYAAQTPLWGSAYAMDIEAMAKLKIPSMLFGPWGKDIHTRWERVNKASLYEK
                     TPAVLKNFIEQMFDK"
     gene            complement(10959..11939)
                     /locus_tag="ACZPCL_04310"
     CDS             complement(10959..11939)
                     /locus_tag="ACZPCL_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161741.1"
                     /translation="MGLLIIFMQKAIGQGIGILYGALGEILTEKSGNLNLGIPGMMYM
                     GGIAGLIGAFLYENSSAAPNGAVGVLISFLCAFLCAALGGLIYSVLTITLRANQNVTG
                     LALTTFGVGFGNFFGGSLSKLAGGVGQISVAVTGAAFKKQIPVLSGLGAVGQLLFSYG
                     FLTYLAIILALVLAFFLAKTKKGLNLRAVGESPATADAAGINVTVYKYLATCIGGGIS
                     GLGGLYFVMEYSGGTWTNNGFGDRGWLAIALVIFALWKPVNAIWGSILFGGLYILYLY
                     IPGLDRGAQEIFKALPYVVTIIVLVFTSLRKKREYQPPASLGLAYFREER"
     gene            complement(11951..13066)
                     /locus_tag="ACZPCL_04315"
     CDS             complement(11951..13066)
                     /locus_tag="ACZPCL_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161742.1"
                     /translation="MSKEMSTVTNKEPLMHISKRDGIETWKSWLIRIIAVLLSLVVCA
                     GVIVALTGLNPIEVYKGIFDGAIGTKRRAWMTIRDTLVLLCIAVGITPAFKMRFWNIG
                     AEGQVLIGGVTSAAMMIYLGNSLPPVVLFPLMFLGSAAAAMIWGVIPAFFKAYWNTNE
                     TLFTLMMNYVATQVITYCIIFWENPKGSNSVGTINSSTKGGWIPQLFGLDYGWNLVIV
                     LALTVAMFIYLKYSKQGYEIAVVGESENTARYAGINVKRVILRTMALSGAICGIAGFI
                     IVGGASHTISTSTAGGRGFTAIIVSWLSKFNTFVMILVSFFLVFMQKGAGQIASQFNL
                     NENASDIITGIILFFILGCEFFINYKVGFRSGKKSGR"
     gene            complement(13059..14591)
                     /locus_tag="ACZPCL_04320"
     CDS             complement(13059..14591)
                     /locus_tag="ACZPCL_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1161743.1"
                     /translation="MSETYAIELKNITKRFGKVTANDKVCLSVKKGEILSILGENGSG
                     KTTLMNMISGIYYPDEGQILVNGKEVTIRSPKDSFALGIGMIHQHFKLVDVLTAAENI
                     ILGLPGKEVLDMKSVAAKINELTSRYGFDLDPNQKIYTMSVSQKQTVEIVKLLYRGVD
                     ILILDEPTAVLTPQETEKLFTVLRNMREAGHSIIIITHKLHEVLSLSDRVSVLRKGAY
                     IGTVQTKDATEASLTEMMVGKKVALNIERPEPVNPQKRLVMEHVTCVDKEGVKTLDDA
                     GFTAYSGEILGIAGIAGSGQKELLETIAGLQPMEGGTIRYYPPEDGEETLSGMSPAAI
                     RKLGIKLSFVPEDRLGMGLVAAMGMTDNVMLRGYKKGIPFFTDRRTPKAMAEQLIKEL
                     EIVTPGVSTPVGRLSGGNVQKVLVGREISSNPRVLMVAYPVRGLDINSSYTIYHLLNE
                     QKKQGAAVICVGEDLDVLLELCDRILVLCGGKVSGIVDGRTATKEEIGLMMTKTGGEQ
                     DE"
     gene            complement(14745..16013)
                     /locus_tag="ACZPCL_04325"
     CDS             complement(14745..16013)
                     /locus_tag="ACZPCL_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1161744.1"
                     /translation="MMGKKIISAALAGVMALSLTACGGGSTQETTAAPAADSKAEETT
                     AAAEGSEAATGEGVAKEDLKVGFIFIGDENEGYTAAHYKGAKEMQEALGLNDDQLIVK
                     WNIPEDETAKDAAMDLADQGCQIVFANSFGHESYVIEAAKEYPEVQFCHATGFQAAGS
                     GLSNMHNFFTSIFEARYVSGVVAGLKLNQMIEDGTVKEDACKIGYVGAYPYAEVISGY
                     TSFFLGVRSVCPSATMEVKYTNSWASFDLEKEAADALISDGCVLISQHADTTGAPTAC
                     EAAGVPCVGYNISMIATAPDQALTSASNNWGIYMTYAVQSVLDGTEIPADWCKGFSDG
                     AVLVTELNDKAVAPGTKEKVEEVEAKLASGELHVFDTSTWTVNGETLDTYKKDGSDIE
                     YISDGYFHESEFASAPAFDILIDGITTIDN"
     gene            complement(16187..16471)
                     /locus_tag="ACZPCL_04330"
     CDS             complement(16187..16471)
                     /locus_tag="ACZPCL_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6809 family protein"
                     /protein_id="HIR1161745.1"
                     /translation="MKNILEQLYAGELVPAELKIEGNEEYETLCRRSLKEIENFTEKL
                     DKENRKEFQNILDTYLELTYLEKRQSFCDGFRIGAGIMCEVFKERSCGVN"
     gene            complement(17143..>18937)
                     /gene="ftsH"
                     /locus_tag="ACZPCL_04335"
     CDS             complement(17143..>18937)
                     /gene="ftsH"
                     /locus_tag="ACZPCL_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1161746.1"
                     /translation="VMVLNALVFPSVMERSVEVPFNEFTTQLSSGNVEQVYVNSDYTE
                     ILYTLNEDKNKPWSAVTYKTGNMTTADDLVNRLESAGVQNYSREIPTQASPLLNFLTT
                     WIFPILMFVIIGQLMGRMMAKRMGGGSSAMTFGKSNAKIYAEKETGVTFNDVAGEEEA
                     KDALKEIVDFLHNPDKYAQIGASLPKGALLVGPPGTGKTLLAKAVAGEAKVPFFSISG
                     SEFVEMFVGMGAAKVRDLFKQANEKAPCIVFIDEIDTIGKKRDGGGMGGNDEREQTLN
                     QLLTEMDGFDGKKGVVILAATNRPESLDPALLRPGRFDRRVPVELPDLQGREAILKVH
                     GRKVKMDESVDYNVIARSTAGASGAELANIINEAALRAVRQGRNLVSQEDLQESVETV
                     IAGYQKKNAVIPADERKIVAYHEVGHALVAACQTHSAPVQKITIIPRTSGALGYTMQV
                     DAGEKYLMSKEEALNKIATFTGGRAAEELIFHSITTGASNDIEQATKIARAMVTRYGM
                     TDEFDMVAMETVNNQYLGGDTSLTCSPETARMIDEQVVKIVRTQHEKALNILKENEDK
                     LHEIAKYLLDKETITGEEFMEIYEKNDADAN"
BASE COUNT         4837 a         4694 c         3960 g         5446 t
ORIGIN      
        1 ttgtacacgt attctcaaaa aacgtcaacc gctttttatt ttttaaagat ggtctgtaag
       61 atcttcctgt attgacaatg caaacattta gatgtactat caactatata gatgtatata
      121 catcttcttt ttagaaagga tcgttaaatc tatggatgat acacagcgta aaataacaaa
      181 aatagccaga gaagctgcca aatttacagt ccagaccatg aaagccgagg gcatcggcac
      241 agctgaattt gactttatcc atctggtccg ccacaaaccg ggaatcaccc aggcagaaat
      301 ccgtgaaacc ttaaagatcg acaaaggagc cgctgcccgc cgggctgcaa gcctagaagc
      361 aaagggctat ctggtacgaa aaccaaatcc tgaagataaa cgcagccagc ttctttttgc
      421 cacagaaaaa gcggaagaac tgaaaaactc caaagcaagt attgaagacg ctttttatga
      481 ctggctcatt gctgatctct cagaagcaga ccgcagggaa ttctgccgca tcctggatat
      541 cctttactgg aaatccaagg aagaacggag agtagacttt acccatgtat cggcatggac
      601 caatgaaaaa ctttcccgga tccccagcga agaaatgacc gatcaaaaaa taccagataa
      661 agaaatacta aataaagaac tgcccccaaa aaaggctccg gaaaaagaac cacaggaagg
      721 agaactctca catgagtaaa caatctgcac cccccatatg caaacatcca gaccatgtca
      781 ccagttattt ttccatgcaa aagggaattt tagctgttat cacagtgtcc ggtcttattt
      841 acaatcttgg acttatggcc cgtccctggt ttgaaggcca gatgatacag tgcctgtttt
      901 ttatccagaa aggccaaagt catttttcag atatgctgaa actggcatct gcttatttta
      961 ttgccatttt tacggtacag atctcccgtt tcatcaaacg tctgtatgtg cgccgcttcg
     1021 ccaacaacat caaccgcagt atgaaacaga cgtattataa caacctggtc catacggata
     1081 cctccgatct gaaaaatgtc aataccggaa atgccatgac aaaagcttta tccgacgtag
     1141 acgcatgttc cgaagggatc cgtaaattta caactgagat cttcgataca ggtgtagcac
     1201 tgttttccta tgttttcatg cttttcgcct acgactggaa actggctctt ttgtgcctta
     1261 tcttccctcc tgtttcctat gtgatcgcag aaaggatgaa agtcgttgtc ctgaaaacag
     1321 gtgctgcctg caaagaaagc gccggacgtt taaatgccgc aaccttagac cgcatccaaa
     1381 acgccattac ctaccgtatt tacggctgtg agacccagag aaataaagat tatgaagccc
     1441 acctggctga ttacgaaaaa gcctctgtac gtgcggatct gccggtagcg gccctgcctc
     1501 ctatttataa gcttatttcc atgaccggcg tccttctcat cttttatttt ggaagtaaaa
     1561 atgtaatggg aaccggctgg acttcctggg atattgccgc tttcagcgct ttcctttcct
     1621 gtttttccag gctgtctgta aaatcttccc atgcagccaa gctttttaat gctatccaga
     1681 aagcccaggt ttcctggaag cgtatccttc cttttatgaa agcaggaaaa gaagacaaac
     1741 ctctgccagc ttccagcctt ggacaggtaa acgcaagatc ccttggattt tcctatgaaa
     1801 acagcgctcc tcttttcaaa gatctgacat tccaggcttc tcctggtgag atcattggta
     1861 ttaccggccc ggttgcctgc ggaaaatcca cattaggacg gatcttcctc tgcgagcatc
     1921 cttacaccgg ctccctcacc tttaacggta aggaattttc ttccctttca ccagatgtgc
     1981 gcaattctct ggtgggatac ttaggacatg aaccggatct gttaagtgat accattaaaa
     2041 acaatgtttt acttggggat caaaaggatc tgtggaaata cttagaagct gtatgcctgg
     2101 aagatgaggt acgtcatatg cctcagggtg aagatacagt aaccggtgaa acgggcctcc
     2161 gtctttccgg cggacagcag aagcggctgg ctctggcccg tacactggcc catccccgcc
     2221 cgatcctgat cttagatgat ccattttccg ccttagacaa gacaacagaa gtagaagtgt
     2281 ttgaacacct gcgtcaaatg gtgccggaca gcattatctt tctcatctcc catcgcctgt
     2341 atctgttctc ggaaacagac ggcgtgatct ggatggataa tggaacagca gaattttctt
     2401 cccataaaaa actgttgaaa gataatagtg cttacagaga attatatcag cttcaaaccg
     2461 actattccgt tgcaaaacca acggaaagtg cgattgaaag ggggatctgc tgactatgaa
     2521 aacagaacat tctgtcctta aggttatttt acgcactgta aaacagaata agggaatgtg
     2581 cgtaacttta tttgctgctg tgttgggatc tgctgtcttt gcccttctcc cgccgctggt
     2641 actggcaaaa gccattgact atcttacagc cggccagacc tttccttttg ctctggctct
     2701 gtcctatttt ggactgcttt tattaacaaa tgctgccacc tctttgcgtg aaagcctttt
     2761 gacgatcttc gggcagcgca ttacgcatgg actgcgcact gttttatgcc agaagatcag
     2821 ccttctgcct gcagactctt ttgtttccca tgactcagga gccattgctt cccgttttgt
     2881 aggtgatgtg gatacagtag aggaactttt tacttccggt atcatcagca tgtttgcaga
     2941 cgcctgccag ctttgcggta ttttctttgt gatctttacg aaaaaccgcg gtctggctat
     3001 tctgctgctt tttgtgctcc ccgctgtatt tttctacacc cgtcatgtac aaaaacgcat
     3061 gcttgcatcc cagttaagta accgtgctgc cattgcaaaa gcctctggtc atgtaccgga
     3121 gacactgcgc tgtatccgca ccattcacaa tcttcacaaa gaaatgtata tggaagaaaa
     3181 atacagtaaa tttctggaag acagctataa ggcaattgat aaaacaaatt tttatgatgc
     3241 tatttattca ccggtcatcc ttatttccaa ttcaattacc atcgccattg ttatgatctt
     3301 atctgctttt ggaaatgggg aagtccagct gtttttcgga atgtctgccg gtactgctgc
     3361 cgctgttatt tcctatatta cccaggtatt tacacctctt gaaagtattg gtatggaaat
     3421 acagaccatt cagtctgctg ctgccggagt acacaggatc aatgaatttt tgcagcttcc
     3481 ggaacgtacc aaagctaggg aaacaaacac ttccagaccg gatccggctg catgcccggt
     3541 agaacttcag aatgtaacct tccgttatga ggaaagtgac ccggatattc tacagtccct
     3601 cagtttttct gtaaaagaag gggaacaggt tactttgacc ggacgtaccg gtgccggaaa
     3661 aagtactatt tttaaactgc ttttaggact ttatacacct cagaaaggaa acgttcttat
     3721 taacggacag cctgccgatg cactctctga aaaccagaga cgaaagatca tcagctgtgt
     3781 agaacagaat ttttccatgg ttcccggaac gatcctggac cagatcacct tgtttgaccc
     3841 ggatatcaca ttggaacagg cccagactgc cgcaggtctt acaggactgg atgaagttat
     3901 acgcagtttc ccggaaggct acaacactcc ctgcaaacca ggtcttttct cccagggaca
     3961 atggcagctg ctttccattg ccagggctgt tgcaaccaac ccccgcatcc ttttattaga
     4021 tgagatcacg gcaaatcttg acgcagacac agaacagacc gttctttctg ctttaaaccg
     4081 agccggaaac ggacgtaccg tgctttccat cagtcaccgg ttatatgaaa aaaatggcgg
     4141 acggcagatt gcgatctgac gcccgctggg atttcatttt tcagctgtag aatgatccag
     4201 gtcactctgg caaatcctac agcttttttc ttatatttct ccaaaatacc gttgaaagga
     4261 ccaccgcgat atagtctgta attgcctgac cgtatatcac gccttctaat cctgcgatca
     4321 gctgcaggat gtaaagcagc gggatcagga ggacgccctg acgcataacg gaaaggatgg
     4381 ttgcaggcaa tgcgtaattt acagactgca tacagttcat atttacaaaa agcatgccga
     4441 tcacaggacc ggaaagcatg tatgcaatca gcatctttac cccataataa accacctcat
     4501 catcactgat gaacatgcgg ataataggct cttttcccac aaaatacagc agtgtggccg
     4561 caactccggc tatcaggcag cagatcttcg taaagcgttc tacttcccgg aagcggctga
     4621 tatttccagc tccatagctg taacccaaca atggctgtac tccgtttgcc agtcccattt
     4681 gcaggaaagt aatgaacata ttggctttaa aaacaatacc gatggctgcc accggtgcat
     4741 ttccatattt taccaaaatc tggttgacta caatggtgga aacgctcatg agaacagaaa
     4801 agatagcagt tgggaagccg gtagtgcaga ctctctgcgg gatattttct cctaaagtaa
     4861 agtcttttgg aagaatggac agctgaccgg atctctttaa gaaataccat acaaagtaca
     4921 gacttgccag gatatttccg atggtagttg caatagccgc accggcagct cccatatgca
     4981 gaccagagat aaacaggggg tctaatacaa tatttgcaat ggttcccacc atgcttccga
     5041 tcatagcttc tttactggca ccttctgccc ggactacaaa gctggctgcc gcagaccaga
     5101 tgataaaggg agcgccccag gcaatgtaat ggatgtaacc tttagcgtat tcataggttt
     5161 ctgtatttgc gccaaaaata tgtaagatgg gggacataaa tgcaaataaa ataactgcaa
     5221 ttatgatgcc tactgctaaa ctggcatagg tgataaatgc ggtagtgttc tttacttttt
     5281 tgggttttcc ctctcccatt gccatggatg ccacggcgct tccgcccatt ccaaacatat
     5341 tggcaaaagc cataaggaga atgaaaatag ggtttgtcag actgactgcc gctacctgaa
     5401 gtgcatctcc tgtctgtcct acgaaaaagg tatctgccat gttataaacg actgtaacca
     5461 gactgctgat cacacttggg atcgccatgg cggctactgc ttttgacacc ttcatagaac
     5521 gcatcagttc ctggtctgta cccgtttctt ttgaatgttc cattttgatc ttcttccttt
     5581 ctgtatttcc ttttcccctc agatgtatat atctgtctgt tatctacggg gaactttcgt
     5641 tattaatttg cttcatccag atttttaagc atacgctctc cgatttcaat aaagatctgt
     5701 ctttcctctt ctgtaatacc ttttaataaa atgctttccg actcgcctat ttcagcagcg
     5761 atcagatgac ggatttcttc ggccttggac gtaaattcga tccgttttaa acgggcatcc
     5821 tgcttttcac tgacacggca gatcagttct ttcttttcca gggcctttaa tacttctgta
     5881 gccgtagatg gacggagacc aaattctttt tctatatctt tctgataaat agaatgtttc
     5941 tgtccttcca ctaaaataaa atttaaaata ttcccctgcg ccccgctcat tcccagtgtt
     6001 tcctgtaaag ctgcagatct gcggcgcaga cggttagata tacggttaat gacttttcct
     6061 gtttccatat gactgtactc ctgtgttttt gtaattagtt cggttcctaa gtattttaga
     6121 taaaaaaatt tcagaagtca aggattattt actgctgtta tatctctgac ttctgaaata
     6181 tgttgtcgtt tattacttat aaataccgca catttatgat cctacgcctg tgctaacagg
     6241 cggaatctcc tattaatcac tgttccagat atttcttcac atattttcct gtataggaaa
     6301 ccgggctttc tgccacttct tcgggagttc ctttggcaat gacagttcct cccttgtcgc
     6361 cgccttcagg accgatatcg atgatatagt ctgctgtttt gatcacatcc aggttgtgtt
     6421 caatgacaac aacggtattt ccaccgtcgg acaggcggcg aaggatctcg atcagcttgt
     6481 ggacatcggc aaaatgaagg cctgtagtag gttcatccag gatataaatg gtctttccgg
     6541 tactgcgctt ggaaagttcg gtagcaagtt tgatacgctg ggcttcacca ccggaaagtt
     6601 cggtagatgg ctgtcccaga cggacataac caagacctac atcatataag gtctggatct
     6661 ttctggtaat ggagggcaca ttttcaaaga atttcaacgc atcttctact gtcatatcca
     6721 ggacatcata gatgctcttt cctttgtatt ttacctccag tgtctcacgg ttatagcgct
     6781 ttccgccgca gacttcacag ggcacgtata catccggaag gaagtgcatc tcgatcttga
     6841 tgataccatc gccggagcag gcttcacagc gaccaccttt tacattgaaa ctaaatctgc
     6901 ccttgttgta gccctttgct tttgcatctg ctgtagaggc aaacaggtcc cggatcaggt
     6961 caaatacacc ggtataggta gccggattgg aacgtggggt acgtccgatc ggggactggt
     7021 caatagctat aatcttatct aactgctcta ctccttcaat gcatttatgc tttccaggaa
     7081 tggtccttgc gcggtttaac ttctttgcaa gggctttata taagatctcg tttacaagag
     7141 aacttttacc ggaaccggat acaccggtca cacaggtcat cacgcccagc gggaaggaaa
     7201 catcgatctt tttcaggtta ttctcttctg ctccccgcac cgtgatccag cccgttggag
     7261 ttctgcgctg atcaggaacc gggatctgca gacggccgct gagataggca ccggttattg
     7321 actccgggtt tttcatgatc tcttctgcag ttcccactgc aacgatctgt ccaccatgtt
     7381 ccccggctcc aggtccgata tccacaatgc agtctgctgc acgcatggta tcttcgtcat
     7441 gttctactac cagaacgctg tttcccaggt cacgcagatg caataaagtt tttaacaatt
     7501 tatcattatc tctctggtga agtcctatac ttggctcatc caggatatag gctactccca
     7561 ccagaccgga tccgatctgg gtagccagac ggattctctg ggcttcgccg ccggaaagtg
     7621 tgcctgtagc acgggaaagg gaaagataat ccagtcccac atcaataagg aaactgatac
     7681 gtgctttaat ctcctttaaa atagaggcac caatggtctg ctgcataggt gtcagcgtca
     7741 gcccatccat aaattcctta aatttcacaa tggacatatt agtagcctgg taaatattca
     7801 gaccgcctac tgtaactgcc agagactcct ttttcagtcg ctgtcccttg caggccggac
     7861 atggggtgat ccgcatatag gtttcatatt ctgccttgga agcttctgaa aaagtctctt
     7921 tatagcggcg gtttacattt tcgatcaggc cctcaaatgc tacatcgtag atgccttcac
     7981 ctctctggct cttatagcgg acttttactt catgaccgcc ggtaccatag atgataatat
     8041 cgtggatctc ctttggataa tcctgaaatg gagtatccag gctgaaatga tatttctctg
     8101 ccagtgcgtc taagatagcc ctggtaaagc ttcctttgtc cgtacaggac tgccatccca
     8161 taaccacaat ggcaccctgg gaaatggaaa gggatttatc cgggatcatc agatcctcgt
     8221 caaattccat cttatagcct aaaccgaagc actccgggca tgctccaaat gggttgttaa
     8281 aggagaagct tcggggctct acctcgtcaa tactgatacc acagtccggg caggaaaaac
     8341 tctggctgaa attcaccggt tccccaccga tcacatctac agtcatcagg ccgttgctta
     8401 aggacataac cgtctcaata gagtcggtca gacggttttc aattcccgct tttacaacca
     8461 gacggtctac aataatatca atattgtgct taatgttttt ctctaatttg atctcttcgg
     8521 aaagttcgta cagatttccg tcaatgcgca cacgcatata gccgcttttt ctggcctgct
     8581 ccagcacttt tacgtgttcg ccctttcttc cacggacaac cggagccaaa agctggatct
     8641 tggtccgctc cggcatagcc atgatctggt ctaccatctg atcaatggtc tgtttgtgga
     8701 tctccctgcc gcactttgga cagtggggga taccaatgcg ggcatagaga agacgcatat
     8761 agtcatagat ctctgtcacg gttcccactg tagaacgtgg atttcggttg gtggatttct
     8821 ggtcaatgga aatggccgga gaaaggcctt cgatgctttc tacttccggc ttttccatct
     8881 gtcctaagaa ctgtcttgca taggaagaca gagactccat atagcgtctc tggccttctg
     8941 catagatcgt atcaaacgcc agagatgact tgccggagcc ggaaagaccg gtgagaacca
     9001 ccagttcatt ccttgggata tctacggata tatttttaag attatgttca tttgcgccgc
     9061 gtatttttat atactgcttt ggcatggtat gactcctttt tctgctgttt cctgaaaata
     9121 tttactcact tctatacctg gtttttccag gcaatataat gctgaaaagc gcagggaaaa
     9181 tttctccctg cgctttttag tataccacat ccctatttat ttatcaaaca tttgttcgat
     9241 aaagtttttt aatacagccg gcgttttttc atacaaactg gctttattta cacgttccca
     9301 acgggtatgg atatctttcc cccatggtcc aaagagcatg gaggggatct ttaatttcgc
     9361 cattgcttct atatccatgg cataggcact gccccataat ggggtctggg ctgcataggc
     9421 agccagttct tctttgtcca ttccgccaca gtagcttaag tcggaaatgc cgcagaaata
     9481 ttcatggcat ttcagggaaa cattctcctt ctctgctgct tttttcatct ctttaaaaag
     9541 aatttttccc tcttttccac tgtctacagc cggataaaat ggaggtgcaa agccaaggat
     9601 catgaccgga ccggagatcc ctgactggtt taacagctgc tccataaaat caagggtggc
     9661 tgatgggaag ttggtctcgc ctttgtccag gcgggctttc tgagtcttgt aggcttctga
     9721 aagccaggca gtaaaggctt ctccctgttc tttgcggcag tatgccagca actcactgac
     9781 tgtaagaact tctgcttctg cagcggcggc tgtgccggga catgccaggg gattgccttc
     9841 acttgtcaga ccagcttttt cttctggaac ctctgctttt tccacagcct tccactgcgc
     9901 ttctattctc cgggcatatt cttcaaaact ttcttttccc agttctttca atcgctttat
     9961 cacttcatcc ggcgtttttt caaacccaag tatgctcatg tatccaccag ccctgaaagg
    10021 cacggacaca tcgtagcctt ctttccggtc acgaagacaa aaccaggttg gcggtgggca
    10081 gatttccgta gcactttttt ctgcgaattc cggtgccagt tctgttttca tgaaaaagct
    10141 gcttaaaacg cctacagagc tgacaccctg gaaacagttt aatacatggg cctttacgcc
    10201 ctggaccaat acagcaggca ttgtttttcc aacagaacct gtataaatgg tctttgcccc
    10261 attttccatg gaagccggtt ccagatcgat cagtgctgta tattccaggt caaagcgttc
    10321 ttttaaatca tttaaaaatg gcacagcatc gcgcattcct gctgagtagc cttcttcatc
    10381 cggtactgtt acgaacagga gatttccaga tatttcaggc gtctcttctg ttccagaagc
    10441 agtttttgtc tcaaagacag ccgcaccaca ctctttccgc tctgcctcaa ccaccagctt
    10501 cccataccag tccagcagcg ccagtccaac agaaagtccg cccttcatat ctgcggtccc
    10561 tctgccaaac atccagtcac cagaaaggaa atcttttttc acttcctctg gcataccagg
    10621 cagagcattt ccagatttta ccagatcttc ccatttttcc acatcataag caagagcacg
    10681 ctcatttcca tactcttctg tgtccaccac gtcataatgg cctgtaagga taatggtctt
    10741 acgggatccg ttccttcctt ttaccagtcc ccatggtaca gaccggccaa agctgtcttc
    10801 cggcagcaga tatttccctg tctgctccgg atactcttta aagtaaggct gctttgccag
    10861 ctcctctgca atatagtctg cgcagctttt ttctgtctct gtattggaag tgctggggat
    10921 ttttaccagt tctcttacat accgttcaat taattctctc atcgttcctc acggaaatat
    10981 gccagtccca gacttgccgg cggctgatat tccctcttct ttctcagact ggtaaatacc
    11041 agtacaataa ttgttactac ataaggcagt gccttaaaga tctcctgtgc cccacggtcc
    11101 agtcccggaa tataaagata caggatataa aggccgccaa aaaggataga accccagatc
    11161 gcatttacag gcttccagag agcgaagata acaagggcaa ttgccagcca gcctctgtca
    11221 ccaaaaccat tattggtcca ggttccgccg gaatactcca ttacaaagta cagaccgcca
    11281 aggccgctga taccgcctcc gatgcaggtt gccagatatt tgtatacagt aacattaata
    11341 cccgccgcat ctgctgtagc cgggctttca cctactgccc gcaggttcag gccctttttg
    11401 gtctttgcaa ggaaaaatgc cagcaccaga gccagaataa tggccagata agttaaaaat
    11461 ccataggaaa ataataactg tcccactgca ccaagaccgg aaagtacggg gatctgcttc
    11521 ttaaaagctg caccggtcac tgcaacggag atctgtccaa cgccgcctgc cagcttggaa
    11581 agagaaccgc cgaagaagtt tccaaaacca acaccaaaag tagtaagagc caggccggtt
    11641 acgttctgat tggcacgtaa ggtaatggtc agaacactgt agatcagacc gcctaaagca
    11701 gcacataaaa acgcgcacag gaaggaaata agaactccca cagccccatt tggcgcagcg
    11761 ctgctgtttt cataaaggaa agcgccgatc agcccggcaa tgccgcccat atacatcatt
    11821 ccgggaatac ccaggttcag gtttccggat ttttccgtta aaatctcgcc taaagcgcca
    11881 tagaggatac cgattccctg tccaatggcc ttttgcataa aaatgatcaa taatcccata
    11941 tgtccgcctc ctatcttcca ctctttttcc cgcttcgaaa tcctaccttg tagttgataa
    12001 agaactcaca gccaaggata aagaacagga tgataccagt aatgatatct gatgcatttt
    12061 catttaagtt aaactgggac gcgatctgtc ctgcgccctt ttgcatgaac accaggaaga
    12121 aagataccag gatcattaca aaggtgttga atttactcag ccaggaaacg ataatggctg
    12181 taaagcctct gcctcctgct gtactggtag aaatcgtatg gcttgcgccg cctacaatga
    12241 taaagcctgc aataccgcag atagcaccag acagagccat ggtgcgaagg ataacacgtt
    12301 ttacattgat gcctgcatag cgggctgtat tttcactttc gccaacaaca gcgatctcat
    12361 atccctgctt gctgtatttt aagtaaatga acatggcaac agtcagagcc aggacgatca
    12421 ccaggttcca gccatagtcc agaccgaaaa gctgcgggat ccaaccgccc tttgtagagg
    12481 aattaatggt tcctactgag ttggagccct ttggattttc ccagaaaata atacaatagg
    12541 taatgacctg ggtggcaaca tagttcatca tcagggtaaa cagtgtttca ttggtattcc
    12601 agtaagcctt gaaaaaggcc gggatcacgc cccagatcat ggctgctgct gcactgccaa
    12661 ggaacataag agggaataat accactggag gaagggaatt tcccagatag atcatcatag
    12721 ctgcggaagt aacaccgccg atcagcacct ggccttctgc accaatgttc cagaaacgca
    12781 ttttaaatgc cggggtaata ccaactgcaa tacataacag cacaagtgta tcacgaatgg
    12841 tcatccatgc acggcgcttg gtaccaatgg caccgtcaaa aatgccctta tacacttcaa
    12901 taggatttaa accggtcaga gctacgatca cgcctgcaca aaccaccagt gacaaaagca
    12961 ctgcgattat gcggatcagc cagcttttcc atgtttcaat tccgtcacgc ttggaaatat
    13021 gcatcagagg ctctttattt gtaactgtac tcatttcctt actcatcctg ttcgcctcct
    13081 gtctttgtca tcataagtcc gatttcttct tttgtagctg ttcttccatc tacaataccg
    13141 ctgaccttgc cgccgcaaag caccagaatg cggtcgcaaa gctccaggag cacatccagg
    13201 tcttccccta cgcagatcac cgctgccccc tgctttttct gctcatttaa taagtggtaa
    13261 atggtatagg aagagttaat atccagaccg cgcaccggat atgctaccat aagcacacgg
    13321 ggattggatg agatctcacg tcctactaat accttctgta catttccgcc ggaaagccgt
    13381 ccaacaggtg tggaaacacc gggcgttacg atctccagct ctttgatcag ctgctccgcc
    13441 atagcttttg gcgttcttct gtctgtaaag aatgggatgc cctttttata gccccggagc
    13501 atcacattgt cagtcatgcc catggctgcc accaggccca ttcccagacg gtcctcagga
    13561 acaaaggaaa gcttgatgcc cagtttgcgg atggccgccg ggctcatgcc ggataaggtc
    13621 tcttctccgt cttccggagg ataataccgg atggttccgc cttccattgg ctgcagtcct
    13681 gcaatggtct ctaacagctc tttctggccg cttcctgcaa tacctgcaat tcccaggatc
    13741 tcgccgctgt aagctgtaaa tcctgcatca tccagtgttt ttacaccttc tttatctacg
    13801 caggtcacat gttccattac cagacgtttc tgtggattta ccggttccgg acgctcaata
    13861 tttaaggcaa ccttcttgcc taccatcatc tctgtaaggg acgcttctgt ggcatctttt
    13921 gtctggactg taccaatata agcacctttt cgcagaacag atacccggtc agaaagggat
    13981 aatacttcat gaagcttatg ggtgataatg ataatggaat gaccggcttc acgcatattg
    14041 cggagaacag taaacagctt ttctgtctcc tgtggggtca gaacagctgt aggctcatcc
    14101 aggatcagga tatccacccc tctgtacaac aatttcacga tctctacggt ctgtttctgt
    14161 gaaacggaca tagtgtagat cttctggttg gggtccagat caaagccata acggctggtc
    14221 agttcattga tctttgccgc aacgcttttc atatccagaa cttcttttcc aggaagtccc
    14281 aagataatgt tttctgctgc agtcagtaca tctaccagtt taaaatgctg gtggatcata
    14341 ccgatgccta aggcaaagga gtcttttgga gaacggatgg tcacctcttt tccatttacc
    14401 aggatctgtc cttcatccgg atagtagata ccggatatca tattcataag agtggtcttt
    14461 ccacttccgt tttctcccag aatggataat atctctccct tctttacgga cagacatact
    14521 ttgtcgttgg ctgttacttt gccaaaccgt tttgtgatgt ttttcagctc aatggcatat
    14581 gtttcgctca ccaggcaaga cctccttttt cagtgctgtg tttatatttt tgtgtgggtc
    14641 tgtgctgcta tttcgcgggt acatactcaa gaagcggagc cagacaaagg gtgactccgc
    14701 ttccaaaacg ttactctaaa agagcagctt tatgatttga cttattagtt gtcaatggtt
    14761 gtgataccgt cgatgaggat atcaaatgct ggtgcagaag caaattcgga ctcatggaag
    14821 tatccgtcag aaatgtattc aatgtcgctt ccgtctttct tgtaggtatc taatgtctca
    14881 ccatttacag tccatgtaga agtatcaaat acatgaagtt cgccgcttgc taacttagct
    14941 tctacttctt ctactttttc cttggttcca ggagcaactg ccttgtcatt taattccgtt
    15001 acaagaactg cgccgtcaga gaagcccttg caccagtcag caggaatttc tgttccgtcc
    15061 agtacagact gtacagcgta ggtcatgtaa atgccccagt tgttgcttgc agaagtaagt
    15121 gcctggtcag gagcagttgc gatcatggaa atgttgtaac ctacacaagg aactcccgct
    15181 gcctcgcagg ctgttggagc accggttgta tctgcatgct ggctgatcag tacgcagccg
    15241 tcagaaatca gagcgtctgc tgcttctttc tccagatcga agcttgccca gctgttagtg
    15301 tatttaactt ccatggtagc ggatgggcat acagaacgta cacctaagaa gaaggaggtg
    15361 taaccactga taacctcagc atatggataa gcgcctacat aaccgatctt gcaggcatct
    15421 tcttttacag tgccatcttc gatcatctga tttaacttaa ggcctgcaac tacaccagat
    15481 acgtagcgtg cctcaaaaat agatgtgaag aagttgtgca tattggaaag tccgctgcct
    15541 gctgcctgga aaccggttgc gtgacagaac tgaacttccg gatattcttt tgctgcttct
    15601 attacataag actcatgacc aaagctgttt gcaaatacga tctggcatcc ctggtcagct
    15661 aagtccattg ctgcgtcttt tgctgtttca tcttccggga tgttccattt tacgatcagc
    15721 tgatcgtcat ttaatcctaa tgcttcctgc atttcttttg cgcccttgta atgagcggct
    15781 gtgtaacctt cgttttcatc accgatgaaa ataaagccaa cttttaaatc ttcttttgct
    15841 acgccttcac cggtagctgc ttctgagcct tctgctgcag cagtggtctc ttctgccttg
    15901 gagtcagctg ccggagctgc tgttgtttcc tgtgtagaac cgcctccaca tgctgttaat
    15961 gagagcgcca taacacctgc taatgctgca ctgatgatct tctttcccat cataatacat
    16021 ctcctccatt tgatgatata tgatcactaa agtctcaaat acagacttta ttatagcatg
    16081 ctctcttaag ttgtcaataa tctttatatt tttctgacac tttttttaca attacacaat
    16141 tttcattata aagtcacact tattaatatt tataagtgac catttattaa ttaactccac
    16201 aggaccgttc tttaaagacc tcacacataa tacctgctcc aatacgaaat ccatcacaga
    16261 aggactggcg tttttcaaga tatgttaatt ccaggtaggt gtctaatatg ttctgaaatt
    16321 ctttccggtt ttctttgtcc agcttttctg taaaattttc tatttctttc agggatcttc
    16381 tgcaaagtgt ctcgtattct tcgtttcctt ctatttttaa ttctgcggga acaagttccc
    16441 ctgcatacag ctgttctaat atgtttttca tgcccctgcc tccttttgtt tttagaatag
    16501 cacagccctg tcgcaaatgc aatcgaacaa aatcttacga ttgcaaatgt gacgaagcta
    16561 tatcatgatg tattattata ttgggattta gttcaatagg gtccaaaatg gctcttccat
    16621 gtataaacgc attcacggca attttgtcca ttttgctcca ccgccgatta ggcaggtctt
    16681 cggcaaactc gctcactgcg ttcgctcaaa cagtgcctcc aacctgccat ctatgctcac
    16741 aaaatggaca aaattgccta ccgaatgctt attatacatg gaagagccat ttctactgta
    16801 ttggatatgt cacataaaaa atgctgtact tatgtacaat ccccctccta catgaaaact
    16861 gcttattggt ttgctcctac atttttattg tcaataacgt agaaagccgc cggtgcacat
    16921 tacagaataa gcatttgttt gaaaatccgt gagtgggcgc gcatagcgtc agaacggaga
    16981 accactgttt gagcacgcaa agcgtgcgag tttggttcgc agttctgaca ataggcgacg
    17041 caagcccacg aacaaaggat tttctcccaa atgcgcatct gtaatgcgta ccgacggctt
    17101 tcggaccctt cggaaatctt tcagacctta gtcgattaaa tcctaatttg catctgcgtc
    17161 atttttttca taaatctcca taaattcttc tcctgtgatg gtttccttat ccagaagata
    17221 ttttgcaatc tcatgaagct tatcttcgtt ttctttcagg atatttaatg ccttttcatg
    17281 ctgggtacgg acgatcttta ctacctgctc atcgatcatt ctggctgttt caggggaaca
    17341 ggttaaagag gtgtctccgc ctaaatactg gttgtttacc gtttccattg ctaccatatc
    17401 aaattcatcg gtcatgccgt aacgggtgac catggctctg gcgatcttgg tagcctgttc
    17461 aatatcattg gacgcgcctg tggtaatgga atggaagatc agctcttcgg ctgcacgacc
    17521 gccggtaaag gttgcgatct tatttaatgc ttcttctttg ctcatcaggt atttttcacc
    17581 agcgtctacc tgcatggtat agcctaaagc accggaggtt cttgggataa tagtgatctt
    17641 ctgtaccgga gcactatggg tctggcaggc agctaccaat gcatgtccta cttcgtggta
    17701 agctacgatc tttcgctcgt ctgcagggat cacagcattt ttcttctggt aacctgcaat
    17761 aacggtttct acgctttcct gaagatcttc ctgggatacc aggtttctgc cctgacggac
    17821 ggctcttaaa gctgcttcgt taatgatatt tgcaagttca gcaccgctgg cacctgctgt
    17881 agatctggcg atcacgttgt aatcaacaga ctcatccatt tttaccttgc ggccatggac
    17941 ctttaagata gcttcacgcc cctgaagatc cggaagttct accggcacac ggcggtcaaa
    18001 acgtcccggg cggagaagcg ctggatcaag ggactcagga cggttggtag ctgctaagat
    18061 aacaacgcct ttctttccgt cgaaaccatc catttctgta agcagctggt ttaaggtctg
    18121 ttcccgctca tcgtttccgc ccattccgcc gccgtcacgc tttttaccaa tggtatcgat
    18181 ttcatcgatg aagacgatac atggtgcttt ttcgttggcc tgcttgaata agtctcttac
    18241 tttggctgcg cccattccta cgaacatttc tacgaattcg gatccggaaa tggagaagaa
    18301 tggtactttg gcttcgcctg ctactgcttt tgctaaaaga gttttaccgg ttcctggagg
    18361 gcctacaagt aaagcgccct ttggaaggct tgcaccgatc tgtgcgtatt tatccgggtt
    18421 atgcaagaag tctacgatct cttttaaggc gtctttggct tcttcttccc ctgctacatc
    18481 attaaaggtt acacctgttt ctttttctgc gtatattttt gcgttggatt ttccaaaagt
    18541 cattgcactg cttccaccgc ccatacgctt ggccatcata cggcccataa gctgaccaat
    18601 gatcacaaac ataaggatcg ggaatatcca ggtggtcagg aagttaagta atggggatgc
    18661 ctgagtgggg atctctctgc tgtaattctg gactcctgcg ctttccagac ggtttaccag
    18721 atcatctgca gtggtcatgt ttcctgtttt ataggtaacc gcagaccacg gtttgttttt
    18781 atcctcattt aatgtataca aaatttctgt atagtcactg ttgacgtaga cctgttctac
    18841 gtttcctgaa cttaactgtg tggtgaactc attaaatggt acttctacgg aacgctccat
    18901 gacagatgga aatactaatg cgttcaatac cattaca
//
LOCUS       DBMDUA010000033        18858 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1760_length_18858_cov_3.189796, whole genome shotgun sequence.
ACCESSION   DBMDUA010000033 DBMDUA010000000
VERSION     DBMDUA010000033.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 18858)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18858)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18858
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1760_length_18858_cov_3.189796"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="ACZPCL_04340"
     CDS             complement(<1..537)
                     /locus_tag="ACZPCL_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIR1161747.1"
                     /translation="MSREEIEQVEKELDEVLEIVQRPVPVKNTGREETVSLQENLQEN
                     DPLQEEMQIETEKEEKEEKEDSPESAAKEQTAVSEEELDLEDDIENGYFHFLRPSDGL
                     IAAFFVPVAVLIILFAQRGIFPFGEECFLRTDMYHQYAPFFSEFQYKLKQGGSLLYSW
                     DIGMGVNFSALYAYYLASP"
     gene            complement(582..1004)
                     /locus_tag="ACZPCL_04345"
     CDS             complement(582..1004)
                     /locus_tag="ACZPCL_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR1161748.1"
                     /translation="MFGKLWKKCVNYETISYLVCGVLTTAVDFVSYTAFRKMSMGVGV
                     SQALSWVAAVLFAYIVNKIIVFRNFNMGPAYLLKEGGAFFAARAISGVATWVLMVVMV
                     KLGGDRGFVYEMFCKLTASVINMVANYVFSKLWIFKKK"
     gene            1194..2120
                     /locus_tag="ACZPCL_04350"
     CDS             1194..2120
                     /locus_tag="ACZPCL_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1161749.1"
                     /translation="MNLSYLKYAVEVEKTGSITKAAQNYYMNQPHLSKIIRELERDLG
                     CPIFDRTSRGMIPTVRGEEFLRYAKGIMEQEAQLDKLCSRNKECPREIRLCGPRASYI
                     SSTLKNFLKEDDRLFTTNVRYKETNSKDVVRLVSSKEFDLGIVRCQVLYEPFFLKMIQ
                     DEHLDSKVLWQFSPKVLMSPSHPLAGKEPLDHLDFSQSVELLQGDIQYPSFTPENTDC
                     VLPDSDPRHTIYIYDRGSQYEFLRHLPGAYMWTSPVPYSTLTEAKLIQRECTRPDNVF
                     KDLLVCRSNYRLSKAETELTQWLGRTISQLSD"
     gene            2440..3627
                     /locus_tag="ACZPCL_04355"
     CDS             2440..3627
                     /locus_tag="ACZPCL_04355"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1161750.1"
                     /translation="MRFTLPRDLYHGKDALESLKTLPGKKAIVVVGGGSMKRFGFLDK
                     VEAYLKEAGMEVQLFEGVEPDPSVETVMKGAKAMQEFKPDWIVAVGGGSPIDAAKAMW
                     VFYEYPDTKFEDLITPFSFPTLRTKAKFCAISSTSGTATEVTAFSVITDYAKGIKYPL
                     ADFNITPDVAIVDPALAATMPKKLTAHTGMDAMTHAIEAYVSTLHCEYTDPLALHAIE
                     MINEYLVASYNGDLDARAKMHDAQCLAGMAFSNALLGIVHSMAHKTGAAYSGGHIVHG
                     CANAMYLPKVIKYNAKEPEAAARYAKIAKFINLPGNTDEELVDALIARIREMNKDLDI
                     PTCIKYYEGGIIDEKEFMEKLPNVAELAVGDACTGSNPRSINPDQMAKLLKCCYYDEE
                     VDF"
     gene            complement(3836..5125)
                     /locus_tag="ACZPCL_04360"
     CDS             complement(3836..5125)
                     /locus_tag="ACZPCL_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIR1161751.1"
                     /translation="MKPYAEMTKEELVALRKDLKAKYHDFQTKDLRLDMSRGKPSADQ
                     LDLSMGMMDVLSSDDDLTCEDGTDCRNYGVLTGIDEAKELLADMMEVNPSTIIIYGNS
                     SLNVMYDTVSRAMTHGIMGNTPWCKQDKIKFLCPVPGYDRHFGITEYFGIEMINVPMT
                     PEGPDMDMVEELVSTDPAIKGIWCVPKYSNPQGYSYSDETVRRFARLEPAAPDFRIFW
                     DNAYGIHHLYDHDQDHLIEILAECKRAGNPDLVYKFASTSKISFPGSGIAAIATSHNN
                     LEDIKAQLKHQTIGHDKVNQLRHVRFFKDIHGMVEHMRKHADIIRPKFEAVEEILERE
                     LGGLGIGEWTKPKGGYFIAFDSMEGCAKDIVAKCKKAGVVMTSAGATYPYGKDPHDSN
                     IRIAPTYPPLGDLIVATELFALCVKLSSVEKLLKGME"
     gene            complement(5350..6789)
                     /gene="gatB"
                     /locus_tag="ACZPCL_04365"
     CDS             complement(5350..6789)
                     /gene="gatB"
                     /locus_tag="ACZPCL_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR1161752.1"
                     /translation="MSKQYETVIGLEVHVELATKTKIFCGCSTAFGGAPNTHTCPVCT
                     GMPGSLPVLNKQVVEYAMAVGLAANCQINQYCKFDRKNYFYPDNPQNYQISQLYLPIC
                     HDGWVEIETESGIKKKVGIHEIHMEEDAGKLVHDEWEDCSLVDYNRSGVPLIEIVSEP
                     DMRSAEEVIAYLEKLRLIIQYLGASDCKLQEGSMRADVNISVREVGASEFGTRTEMKN
                     INSFKAIAHAIEGETRRQIELIEDGRKVIQETRRWDDNKESSKAMRSKEDAQDYRYFP
                     DPDLTPVVISDEWIARIRAAQPELRTEKMVRYISEFDLPQYDTQILTNSKHMADVFEE
                     TVKLCGKPKEASNWLMVEAMRLLKEHEMDPDDMGFSPANLAKLIQMVAAGEINRTVAK
                     TVFEEIFEHNVDPAVYVEEKGLKVVNDEGALKATIEGILAANPQSVADYKGGKEKAMG
                     FLVGQTMKAMKGKADPGMVNKLLKELLAK"
     gene            complement(6803..8272)
                     /gene="gatA"
                     /locus_tag="ACZPCL_04370"
     CDS             complement(6803..8272)
                     /gene="gatA"
                     /locus_tag="ACZPCL_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR1161753.1"
                     /translation="MSILDLTALELGKKIKAGEITSLQATEAVIKQIKAVEEQVHSYV
                     TLDEEGAMKRAKEVQAQIEAGTLTGPLAGVPAAIKDNMCTKDMRTTCSSKILENFVPT
                     YTAEAVLNLEKAGAVILGKTNMDEFAMGSTTETSYYGVTRNPWNTEHVPGGSSGGSCA
                     AVAANECFYALGSDTGGSIRQPSSFCGVTGIKPTYGTVSRYGLIAYGSSLDQIGPVAK
                     DVSDCAAILEAISSHDLKDSTSMARTDCDFTSALKDDVKGMKIGIPSSYFGEGLDEEV
                     RAAILKAADILKEKGAIVETFDLGLVDYAIPAYYVIASAEASSNLSRFDGIKYGYRTK
                     EYEGLHNMYKKTRSEGFGPEVKRRIMLGSFVLSSGYYDAYYLKALRTKALIKKEFDKA
                     FAKYDLILAPASPDTAPKLGASLSDPLKMYLGDIYTISVNLAGLPGMTVPCGMDSKGL
                     PIGMQLIGDCFKEKNIIRAGYAFECTRKYEMPKLAKVSD"
     gene            complement(8284..8576)
                     /gene="gatC"
                     /locus_tag="ACZPCL_04375"
                     /pseudo
     CDS             complement(8284..8576)
                     /gene="gatC"
                     /locus_tag="ACZPCL_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
     gene            complement(8746..10077)
                     /gene="aspS"
                     /locus_tag="ACZPCL_04380"
     CDS             complement(8746..10077)
                     /gene="aspS"
                     /locus_tag="ACZPCL_04380"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HIR1161754.1"
                     /translation="MEFVEGVKAKETLGIAQILEGSYEGKKIRMNGAVHNVRDMGEVA
                     FVILRKAEGLVQCVYEEGVTNFDIHDLKEESAVEVLGTVKAEERAPHGFEIRLEEIKV
                     LSQPAEPMPIPISKWKMNTSLETKLALRPVSLRNVRERAKFKIQEGIVRGFRDYLFTQ
                     GFTEIHTPKIVARGAEGGSNVFKLNYFNKKAELGQSPQFYKQTMVGVYDRVFEAAPVF
                     RAEKHNTTRHLNEYTSLDFEMGYIDGFEDIMAMETGFLQYTMALLEKEYKKELDMLGV
                     TLPDVSKIPAVRFDKAKELVAEKYNRRIRNPYDLEPEEELLIGRYFKEEYGSDFVFVT
                     HYPSKKRPFYAMDDPADPKFTLSFDLLFKGLEVTTGGQRIHDYGEILKKMEKRGMDPE
                     DIASYLMIFKYGMPPHGGLGIGLERLTMRLLDEQNVRETALFPRDVTRLEP"
     gene            10743..12458
                     /locus_tag="ACZPCL_04385"
     CDS             10743..12458
                     /locus_tag="ACZPCL_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_277934961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HIR1161755.1"
                     /translation="MAYFLKKTNNKKGTYLQIYSSFYDPERRHTAHKAYKPIGYVHEL
                     QAKGIDDPISFYKEEVIRLNQEANAAKAAKKAKQISDDSPEKLIGYFPMKNINDKLSV
                     KKYIDLMQTATDFRFNVFDMISALVYARLVQPCSKSKTYDEVIPKLFESYDFSLNQLY
                     DGLEYIGCEYEKIIEIYNHQIQQMYQFDTSHTYFDCTNFYFEIDREDDFRKKGPSKEN
                     RKEPIVGLGLLLDANQIPIGMKLFPGNQSEKPVIRNIIDDLKKRNSVSGRTIQIADKG
                     LNCAENIFHALKNGDGYIFSKSVKMLPETEKTWVLLPNNYRDVKNAAGETLYRIKECV
                     DEFEYKFTESETGSLKKFRITEKRIVTFNPKLAKKQIYEINKEVEKARLLKASQAKKS
                     EYGDSAKYVIFTAADKKGNDTDGKVKVTMNEELIQKSLELAGYNMLVTSEISMKDKDV
                     YNAYHNLWRIEESFRIMKSQLDARPVYLQKQDTIVGHFLICYLAVLLTRLLQFKILKN
                     GYSSEELFEFVRDFRVAKISDRKYINLSHGTSFIRELSELCSLPLTSYFLNEGDIKKM
                     LSHRF"
     gene            12661..13791
                     /locus_tag="ACZPCL_04390"
     CDS             12661..13791
                     /locus_tag="ACZPCL_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1161756.1"
                     /translation="MNSLDKRLQMSGFITFFLSGICSISSGIVVSLLQERYGFDYNVT
                     GTLLSIMSIGNLMAGFATGVLPAKIGLKATIITLSAGYELGYLWLGISGWMSILVLSF
                     LLIGFAKGSVINMCTILVSNHSENRAKGMNIMHSCYALGALICPFVIAGAGLIGPVVP
                     MFTLGFLGLIMWIIFHLTPFEKKTAGKKEKTDWGFLKSKRFWLLTGLIFCQNAAETSV
                     NGWLVTYFKGSGILSGTLSAYTVTVMWTATLIGRLLIAFVFPLKNPYTAMIKMGIGCT
                     VFYIGLMMANNQVAAILLLFAFAFSMAGMNPTAVACAGKMTSVASIGVLLPAASFGGI
                     LMPWIIGIVSNIAGLKMGMAMNIISCVGMLIFCIAVKNLNEE"
     gene            complement(14052..14513)
                     /locus_tag="ACZPCL_04395"
     CDS             complement(14052..14513)
                     /locus_tag="ACZPCL_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR1161757.1"
                     /translation="MNILFFLTPKSEVAYISEEDTLRQALEKMEYHKYSAIPIISRTG
                     RYIGTLTEGDLLWGIKNQYHLDLKGAEKIPITAIKRRLDNRPVKADADMEDLIGKALN
                     QNFVPVLDDQKCFIGIITRKDIIQYFYKKSQQPEEKWPKRQKEEKSETVVG"
     gene            complement(14630..15394)
                     /locus_tag="ACZPCL_04400"
     CDS             complement(14630..15394)
                     /locus_tag="ACZPCL_04400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR1161758.1"
                     /translation="MKDKVNQMFKDKLFLVMMVIGLLTAVAAVGVLTVSNTDEKGQSP
                     YLEVPGGEIIAQDSVPETAQKEEKAEEKISEIEASKVPQENYAQKEEKNPNTKDDLAA
                     EAGAGKQAAKALVLNFSDTSVLTWPVRGNVILDYSMDQTIYFPTLDQFKCNPGIVIQA
                     EVSDPVAAPANAKVLEVGSNEEIGNFVVLDLGNEYTATCGQLKEISAVPGEYLEAGQI
                     LGYVAEPTKYYSVEGVNVFFELKHQEKTLDPLDQME"
     gene            complement(15436..16380)
                     /locus_tag="ACZPCL_04405"
     CDS             complement(15436..16380)
                     /locus_tag="ACZPCL_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR1161759.1"
                     /translation="MEKWMVVLVAAGFLIPYGTVLAVTGNIHGAEYVRQQEKEAAGKY
                     RILLDREENGGYLSLEEYLPSVLAAQISPDSEMEALKAQAVIARTYIRRQMGEEKEIS
                     ELSLDLDRKPREELKKIWGKTEFPEKYSRLEEAVIQTNGIVMTYDGEMIAPLFCAVTA
                     GSTRDGDEGYPYLKSVSCPDDEKAADFISFVSLSAKQAAAALNEIPGKEGEVRQVRPE
                     AFPGKVQTVKRDTAGYVQEIQIDECSFTGEEAAGAFGLSSPCFFTEPQENGIGITVKG
                     KGHGYGLSQNEAEAKAEDGWDFKEILEYFYSGIAFFTE"
     gene            complement(16456..17496)
                     /locus_tag="ACZPCL_04410"
     CDS             complement(16456..17496)
                     /locus_tag="ACZPCL_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR1161760.1"
                     /translation="MTNYRKYTDYIIEQAEKLLSIDSPSGYCRQVTDYLLEELDRLGI
                     QAKRTVKGGVVASFGGKNKEDGILLEAHCDTLGGMVAEIKANARLRLTNIGGMKPANA
                     EAENVRIITKADGIYEGVCQLVNASVHVNGDYEKTERNWDTVEVLIDEPVSSKEDALK
                     LGIMPGDYVCFEPRTRVTSSGYIKSRFLDDKLSAAILLAYGKYLKDEKIEPQRAVYAH
                     FTIYEEVGHGGSASVPDGVTEAWSVDMGCVGEGLMCTEREVSICAKDSGGPYNYEIVS
                     HLVQLAKENNIGFAVDVYPHYGSDVETTLRAGNDIRHALIGPGVYASHGYERSHRDGA
                     ENTFRLIQAYIG"
     gene            complement(17574..>18858)
                     /locus_tag="ACZPCL_04415"
     CDS             complement(17574..>18858)
                     /locus_tag="ACZPCL_04415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR1161761.1"
                     /translation="ITGILQVLACVIMNKFDISNPNIILFVILSAVLVQFGYGAGMLC
                     GFITYIYSMYFFSTDHSFFYFDASNRDKIMVVIFGIIANILIVGSLKARMEKSNKERI
                     HQLEVATTLNKCAVELSADRDIHTAIYNLLGIINQYFQADRSYIFDIDHEKQIVINTY
                     EYTAEGVTRQIDNLQSVPLSVIAVWMDKFENGQVYYTADAEQEKGYPTYEMLVEQNIK
                     RLLTVPLKKNGKVTGFIGVDNPKVHFDDATLLLSLQYFIVNSQSSQRQQERLQFLSFR
                     DMLTGLYNRNKYMKVLETFEKYPVCDTGVAYIDLNGLKQINDNLGHEAGDRLLCDAAK
                     EILRTFPENSYRIGGDEFVIILPESGKAEFEEQMEQVQEDLKQAHISYSMGLEWKKEG
                     MLESMLKAAEQRMYAEKNAYYKLRGRDRRCPERSV"
BASE COUNT         4955 a         4458 c         3627 g         5818 t
ORIGIN      
        1 cggacttgcc agataataag cataaagagc cgagaaattc actcccatac caatatccca
       61 gctgtacagc aagcttcctc cctgttttag cttgtactga aactcagaaa agaagggcgc
      121 gtactggtga tacatgtccg ttcttaaaaa gcactcttca ccaaaaggga agatgcctct
      181 ctgggcaaac aggatgatca ggacagctac cggcacgaaa aaggctgcga tcaggccatc
      241 acttggacgc aggaaatgga agtaaccatt ttctatatca tcctccagat ccagttcttc
      301 ttctgaaact gccgtctgct ccttcgctgc gctttcaggg ctgtcttctt tttcttcttt
      361 ctcttccttc tctgtttcta tttgcatttc ttcctgtaat ggatcatttt cctgtaaatt
      421 ttcctgcaaa gatactgttt cctccctgcc tgtatttttc accggaaccg gtctctggac
      481 tatttcaagg acctcatcca gttctttttc tacctgttct atttcttcac gactcactgc
      541 attcaccttt ctttatagcc gcttttatat attatttcca gttatttctt tttaaagatc
      601 caaagtttgc tgaatacata atttgcgacc atattgataa cagaagctgt caatttacag
      661 aacatctcat aaacaaaccc tctgtctccc cccagtttta ccatcacaac cattaagacc
      721 caggtagcca ctcccgaaat agcccttgcc gcaaaaaagg caccgccttc ttttaataag
      781 tacgccggcc ccatgttaaa attccggaat acgatgatct tatttacaat ataagcaaat
      841 aagactgctg ccacccagga aagagcctgg gacaccccta cacccatgga cattttccga
      901 aatgctgtat aggaaacaaa atccactgct gtagtcagca caccacatac cagataactg
      961 atggtttcat agtttacgca ttttttccat aattttccaa acacgatttt tctcctgttt
     1021 ctgatcttac cattttttct ctgaccagtt ttttctatta tatggtagcc gttcttaatt
     1081 gtaaagttaa ttagcgttta atctgttcac caaactataa atgttttgaa aagaatggtt
     1141 atctacgtta taatgttcac atagaaaata accagaatat ctggaggaat tatatgaatc
     1201 tttcctattt aaagtatgcc gtagaagtag aaaaaaccgg ctctattaca aaagcagccc
     1261 agaactatta tatgaaccag ccccatttaa gcaagatcat ccgtgaactg gagcgtgatc
     1321 ttggatgtcc catctttgac cgtaccagca ggggaatgat ccctactgtc cgtggagaag
     1381 aattcctgcg ctacgcaaaa gggatcatgg aacaggaagc ccagttagac aagctctgct
     1441 ccagaaataa ggaatgtccc cgtgaaattc gtctttgcgg tccccgcgct tcttatatat
     1501 ccagcacttt aaagaatttt ttaaaagaag atgaccggct ttttacaacc aatgtgcgct
     1561 ataaagaaac gaactccaag gacgtggtcc gtctggtctc ttccaaagaa tttgacttag
     1621 gcattgtccg ctgtcaggta ctttacgaac catttttcct taaaatgata caggatgagc
     1681 atttggacag caaggtctta tggcagttct ctcccaaggt acttatgtcc ccctcgcatc
     1741 ctcttgcagg aaaggagcct ttagaccacc ttgatttttc ccagtccgta gaactgcttc
     1801 agggagatat ccagtatcct tcttttaccc cggaaaatac tgactgtgtc cttccggaca
     1861 gtgatccccg ccataccatt tatatttatg accggggcag ccagtatgaa tttctccgcc
     1921 atcttccagg tgcctatatg tggacttctc ctgttccgta cagcaccctt acagaagcaa
     1981 aactgatcca gagagaatgt acccgcccgg ataatgtatt caaggacctt ttagtctgtc
     2041 gcagcaacta ccgcctgtcc aaagcagaaa cagaactgac ccagtggctg ggccgcacta
     2101 tcagccagct gtctgactaa ctggctgctc tgttttgtaa gaccattttt atcaggtctt
     2161 aaaatatcgt cttgaaaata acaatattct cagtaacaga gctggccatt ttttcgttta
     2221 tttttctgtt actgagtatt tttttctttc tgctttatat ttttattcac atttcacaat
     2281 tttcttctca ttaaccagtg atatacttac aggaatacta ttattccatt tttgcatcta
     2341 gtcaagcttt tatcaatgtg ctactatata gctgttaaag aaataacgaa cagatacata
     2401 tagtcaatca cacaactata tttttaggag gtcataatta tgagattcac attaccaaga
     2461 gatttatacc atggaaaaga tgcactggag tccttaaaga cactgcctgg caagaaagcc
     2521 attgttgttg taggcggcgg ctccatgaag cgtttcggtt tcctggataa agtagaagct
     2581 tacttaaaag aagcaggaat ggaagttcag ttatttgaag gcgttgagcc agatccttct
     2641 gtagagaccg ttatgaaggg tgcaaaagct atgcaggaat tcaaacctga ctggatcgta
     2701 gctgtaggtg gtggttcacc aatcgatgct gcaaaggcta tgtgggtatt ctatgagtat
     2761 cctgatacca agtttgaaga cctgatcaca ccattcagct tcccaacact gcgtacaaaa
     2821 gctaaattct gtgctatctc ctctacctcc ggtacagcta ccgaagttac tgcattctct
     2881 gtaatcacag attatgctaa gggaatcaaa tatcctctgg ctgacttcaa catcacacca
     2941 gatgttgcta tcgttgaccc ggctttagca gctactatgc ctaagaagct gaccgcacat
     3001 actggtatgg atgctatgac tcatgcgatc gaggcttatg tttccacact gcattgcgaa
     3061 tacaccgatc cattagcact ccatgcaatc gaaatgatca acgaatatct ggttgcttcc
     3121 tacaatggcg atctggatgc ccgtgctaaa atgcatgacg cacagtgctt agctggtatg
     3181 gcattctcta acgcattatt aggtatcgtt cactctatgg cacacaagac cggtgctgct
     3241 tactccggcg gacatatcgt tcatggatgt gctaacgcaa tgtacctgcc aaaggttatt
     3301 aaatacaacg caaaagaacc tgaagcagct gcaagatacg ctaagattgc taaattcatc
     3361 aacctgccgg gaaatactga tgaagaactg gtagatgctc tgatcgcaag gatccgcgag
     3421 atgaacaagg atctggatat tcctacctgc atcaagtact atgaaggcgg aatcatcgat
     3481 gagaaagaat tcatggagaa actgcctaat gttgcagaac tggcagtagg cgatgcctgc
     3541 accggctcca acccaagaag catcaaccca gatcagatgg ctaagctgct taagtgctgc
     3601 tactatgatg aggaagttga tttctaattc ataagggatc tgaaaaattc agaattcaat
     3661 aaaaactttt taagggctgc agaaaatgat ctgcagccct tattaattgc catgcactaa
     3721 aaaactgccc ggtcacattt ctgtcaccag gcagtttata cgtttttatg actcacaaac
     3781 ccgcacctaa cggtgctcta ctgtctgcta cgcagacggt ttgctcatga atggatcatt
     3841 ccatcccttt taacagcttc tcaacactgg aaagctttac gcacagtgca aacagctctg
     3901 tagccacaat caaatcgccc agaggaggat aggttggtgc aatacggata ttgctatcat
     3961 gaggatcttt tccgtatgga taggttgctc cggcagaggt cataacaact ccggctttct
     4021 tgcattttgc tacgatatct tttgcacagc cttccatgga gtcaaaggcg atgaagtagc
     4081 caccctttgg tttggtccac tcaccgatgc caagaccgcc aagttctctt tccaggatct
     4141 cttctactgc ttcaaatttt ggacggatga tatcggcatg ttttctcata tgctctacca
     4201 ttccgtgaat atccttgaag aaacgtacat gacgcagctg gtttactttg tcatggccga
     4261 tggtctggtg ctttaactga gccttgatgt cttccaggtt gttgtgggaa gtagcaatgg
     4321 ctgcaatacc ggaacctggg aaactgatct tggaagtaga tgcaaatttg taaaccaggt
     4381 ctggatttcc ggctctcttg cactctgcaa ggatctcgat cagatgatcc tggtcatggt
     4441 catacaggtg atggatgccg tatgcattat cccagaaaat acggaaatca ggtgcagccg
     4501 gttccagtct tgcaaaacgg cgtactgttt catcagagta ggaatatccc tgtgggttgg
     4561 aatacttcgg aacgcaccag atacctttga tggcaggatc agtggaaacc agttcctcta
     4621 ccatatccat atcaggacct tctggagtca taggaacatt gatcatctca atgccaaaat
     4681 actcggtaat tccaaagtgg cggtcatagc caggaaccgg gcagaggaat ttgatcttat
     4741 cctgcttgca ccatggagta tttcccataa ttccgtgggt catggctctt gaaaccgtat
     4801 catacataac gtttaagctg gagtttccat aaatgataat ggtagaagga ttaacctcca
     4861 tcatatctgc cagaagttct tttgcttcat caataccggt aagtactccg tagttacggc
     4921 agtctgtacc atcttcacag gtcaggtcat catcactgct gagcacgtcc atcatgccca
     4981 tggacagatc cagctgatct gcacttggct tacctctgga catatccagt ctcagatcct
     5041 ttgtctgaaa atcgtgatat tttgccttca ggtctttgcg aagggctacc agttcttctt
     5101 tggtcatctc tgcatacggc ttcataattt cctcctctat atgtgttctt aagacagaca
     5161 tatgtcagtg ctataaaaac attttctttc attgctataa aatatacacc aaagtattta
     5221 ttccgtcaac acaaaatttc cttatgaagc taaaactttg acttatggtt agatgaaact
     5281 atacatattt ttacttataa cctgacaaat tccccggtaa accggggaat ttaactgtat
     5341 aaatctcttt tactttgcca gcagttcctt caacagttta tttaccattc cagggtctgc
     5401 cttgcccttc atagccttca tagtctgtcc taccaagaag cccatggctt tttcttttcc
     5461 gcccttataa tcggctacgg actgcggatt tgccgccaga atgccttcaa tggtggcctt
     5521 taaagcacct tcatcattta ctacctttaa gcccttttct tctacataaa cagccggatc
     5581 cacattatgc tcaaagatct cttcaaatac agtcttcgcc acggtacggt taatctcgcc
     5641 agctgctacc atctggatca gctttgccag atttgcaggt gaaaagccca tatcatccgg
     5701 gtccatttca tgttctttca gcagacgcat agcttctacc atcagccagt tggatgcctc
     5761 ttttggcttt ccacaaagct ttactgtttc ctcaaataca tctgccatat gcttggaatt
     5821 tgtaaggatc tgtgtatcat actgaggcag gtcaaattca ctgatataac ggaccatttt
     5881 ctcggtacga agttctggct gtgctgccct gattcttgcg atccactcat cactgatcac
     5941 caccggtgtc aggtccggat ccgggaaata gcggtaatcc tgggcatctt ccttagaacg
     6001 catagccttt gagctttctt tattatcatc ccagcgtctt gtttcctgga tgaccttcct
     6061 gccgtcttcg atcagctcga tctggcgtct tgtttcccct tcaatggcat gtgcaatagc
     6121 cttaaaagag ttgatattct tcatctcagt cctggtgcca aattccgacg ctcccacttc
     6181 acggacagat atgtttacgt cagctcgcat ggaaccttcc tgaagcttac agtcagaagc
     6241 tcccagatac tggatgatca gacgaagttt ttccagatat gcgattactt cctctgcgct
     6301 tctcatatcc ggttcagaaa cgatctcgat cagcggtaca ccgcttcggt tgtaatctac
     6361 cagggagcag tcttcccact cgtcatggac cagctttcct gcatcctctt ccatgtggat
     6421 ctcatggata ccgacctttt tctttatgcc ggactctgtt tcgatctcta cccagccatc
     6481 gtgacagata ggcaggtaca gctgtgagat ctggtagttc tgtggattat ctggataaaa
     6541 atagttttta cggtcaaatt tgcagtactg gttgatctgg cagtttgcag ccagacctac
     6601 tgccatggca tattctacca cctgtttatt taatacaggc agagaaccgg gcataccggt
     6661 acataccggg catgtatggg tatttggcgc tcctccgaaa gcggtagagc agccgcagaa
     6721 tattttcgtc tttgttgcca gttctacatg gacttccaga ccgatgacgg tctcatactg
     6781 tttactcatg gcttactcct cctcagtcag atacttttgc cagcttaggc atttcgtatt
     6841 ttctggtaca ctcaaatgca tatcctgcgc ggataatgtt cttttcctta aagcagtcac
     6901 cgatcagctg cattccaatc ggaaggccct tagagtccat gccgcatgga acggtcattc
     6961 caggaagacc agccagatta acggaaatcg tataaatatc acccagatac atttttaatg
     7021 gatcacttaa ggaagctccc agctttggtg ctgtatcagg agatgcagga gccaggatca
     7081 ggtcatattt tgcaaaagct ttatcaaatt ctttcttgat cagagccttg gtacgcaggg
     7141 cctttaaata ataagcgtca taataaccgg agcttaatac gaaagagcca agcatgatac
     7201 ggcgttttac ttccggtccg aagccttcag aacgtgtctt tttatacatg ttgtgaagac
     7261 cctcgtattc tttggtacgg tagccatatt ttatgccgtc aaaacgtgaa aggttggagc
     7321 ttgcttctgc agaagcaatg acgtaataag caggaatagc ataatctacc aaacctaaat
     7381 caaaagtttc tacaatagcg cctttttcct ttaaaatatc tgctgccttt aaaatggctg
     7441 cccttacttc ctcatccagt ccttcaccaa aatagcttga tgggataccg atcttcattc
     7501 cctttacatc atcctttaat gcagaggtaa aatcacagtc tgtacgggcc atggaagtgc
     7561 tgtcttttaa atcatgagaa gaaatcgctt ccagaatcgc tgcacagtcg gaaacgtcct
     7621 ttgcaaccgg tccgatctgg tccagggaag aaccataggc gatgaggccg taacgggata
     7681 cagttccgta ggttggttta atgccggtaa cgccgcagaa ggaacttggc tgacggatag
     7741 aaccgccggt atcagagcct aatgcataaa agcactcatt ggccgctaca gctgcacagg
     7801 agccgcctga agagcctcca ggtacatgtt ctgtattcca tgggttgcgg gtcacaccgt
     7861 aataagaagt ctctgtggtg cttcccatgg caaattcatc catattggtc tttccaagaa
     7921 taacggctcc tgctttttcc aggtttaaaa cagcctctgc tgtataagtt ggcacaaaat
     7981 tttcaaggat ctttgagctg caggtagtgc gcatgtcttt agtacacatg ttatccttaa
     8041 tagcagccgg tacacctgcc agtggacctg tcagtgttcc tgcctcgatc tgggcctgaa
     8101 cctcttttgc acgcttcatg gctccttctt cgtctaacgt aacataagaa tgaacctgct
     8161 cttccactgc cttgatctgc tttatcactg cttctgtggc ctgcaaagaa gtgatttctc
     8221 cggcctttat cttttttccc agttccaggg ctgttaaatc taatatactc atctggccgc
     8281 ctcctattct actgtcttag gcaccatgaa agctccatcc ttctttgcag gtgcattggc
     8341 aagtatctcg tctctgtcat ccccgtttgt cactacatct tcacggaata cattatttac
     8401 gggaaataca tgagacatag gctctacgcc agttgtatcc agttcattta atttatcaat
     8461 ataatcaagc attcttccca ttgttttttt atttcttctt tttcttctgg tgaaagctcc
     8521 agttttgcca gaatgcccac atactctatg gtatcgtcac tgatcacatt tgccatggtt
     8581 ttgtttcctt tctgaagtct cttttaatct ctttccaaag actttttcca aattttttta
     8641 tctaaattgc tttatctaaa ccagatctga accggttcct tctataatat atgctatata
     8701 atatataaga agtattttaa tatttttata agtctttctt atgaactatg gttccagtct
     8761 tgttacatct cttgggaaga gcgctgtctc tcttacgttc tgctcatcta aaagacgcat
     8821 ggtcagtctc tctaagccga ttcccagtcc gccatgtggc ggcattccat atttaaaaat
     8881 catcagataa gaggcaatgt cttccggatc catgccacgt ttttccattt ttttaaggat
     8941 ctcgccataa tcatgaatac gctgtccgcc tgtggtcact tccagaccct taaataacag
     9001 gtcaaagctt aatgtaaatt tcggatctgc cgggtcatcc atagcataaa atggacgttt
     9061 cttagatgga taatgggtaa caaatacgaa atcactgccg tattcttctt taaaataacg
     9121 gccaatgaga agttcttctt ccggctctaa gtcatatggg tttctgatcc gcctgttgta
     9181 tttttcagct accagctctt ttgctttgtc aaaacggact gcggggatct tacttacatc
     9241 cggaagtgtg acacctaaca tatccaactc ttttttgtac tctttttcca gaagtgccat
     9301 ggtgtactgt aaaaatccgg tctccattgc cataatatct tcaaaaccat cgatatagcc
     9361 catttcaaaa tccaggctgg tatattcgtt taaatgacgg gtggtgttgt gcttttctgc
     9421 acgaaacaca ggagctgcct caaatacacg gtcgtaaacg cctaccatgg tctgtttgta
     9481 aaactgtggg ctctgtccta attctgcttt tttgttgaaa tagtttaatt tgaatacgtt
     9541 ggatccgcct tcagcgcctc ttgctacgat ctttggtgtg tggatctctg taaagccctg
     9601 ggtaaataaa taatccctga agcctcttac aatgccttcc tgaatcttaa atttagcacg
     9661 ttctcttacg tttcttaaag ataccgggcg cagggccagc tttgtttcca gggatgtgtt
     9721 cattttccat ttgctgatag ggattggcat tggctctgca ggctgggata atactttgat
     9781 ctcttctaag cggatctcaa aaccatgagg agctctttct tctgccttaa cagtaccaag
     9841 gacttccact gcggattctt ctttcaggtc gtggatatca aaatttgtga cgccttcttc
     9901 gtatacgcac tgtaccagtc cttctgcttt tcgcaggatc acaaaggcaa cttcacccat
     9961 gtcacggaca ttgtgtacag caccattcat acggatcttt tttccttcat aggaaccttc
    10021 taagatctgt gcaattccaa gtgtttcttt tgctttgacg ccttctacaa attccatcat
    10081 atttatttct ccttcccaac ggtgtacttt gaaagtacat cttacggttc cggagatttt
    10141 tgtataaaaa aacgccccgg aacacattta ctgtattccg ggacgggatc ttcttttccc
    10201 gcggtaccac ccgcattgag aaaccttcag gatgctctta agacttctcc tctttttcgc
    10261 atgtaacgta tgccacggat atacctacta tcagtccttc cagccggttg catccccttc
    10321 tggtaaaacc gcttcgatat accagctcca gagtgttccc tttatccagt cctgcgcggc
    10381 ggcgctctca gtcggtgacg cccccttcct gttacatttc tcagacaaga gtctctttca
    10441 tagctgtttt ttatttgttt tataaattaa tactccaaaa gcagaaagtc aagatgtttt
    10501 tttgtatttg tctaattttt gttgattttt ttaaatactg tcctctcatt gacgttgcta
    10561 ttgacaaatg atacccgtct ttaggattta gcactttatt ttttgagagt taatgaaata
    10621 tattttatcc tcaagtcagc atatttgctg gcttttttct ttgttttaaa aacttgacaa
    10681 attttctttt ttatacttgc ttttatggtg ctatggtgct atactataag agggtgactt
    10741 ttatggcata ttttcttaaa aaaactaaca acaaaaaagg tacttatctt cagatttatt
    10801 caagctttta cgatcctgaa cgcaggcata ctgcccacaa ggcttataaa cctattggtt
    10861 atgttcatga gcttcaggca aagggcattg acgatcctat ctccttctac aaagaagaag
    10921 ttatcagact gaaccaggaa gcaaatgctg caaaagccgc aaaaaaagcg aagcagattt
    10981 cagatgattc gccggaaaaa ctcattggct atttcccgat gaaaaacatt aacgacaagc
    11041 tttctgtcaa aaagtatatt gatctcatgc agaccgctac tgacttccgc tttaatgttt
    11101 ttgacatgat ctctgccctt gtttacgcaa ggcttgtcca gccatgttca aaatcaaaga
    11161 cttatgacga agtgattcca aagctttttg aatcctacga tttttcttta aaccagctct
    11221 atgacgggct ggagtatatc ggttgcgaat acgaaaagat cattgaaatc tacaatcatc
    11281 agatccaaca gatgtatcag tttgatacct ctcatactta ctttgactgc actaactttt
    11341 actttgagat cgacagggaa gatgatttcc ggaaaaaagg accttccaag gaaaacagaa
    11401 aagaaccgat tgttggtctg ggactcctgc ttgatgcaaa tcagattccc atcggcatga
    11461 agctgtttcc gggcaatcag agtgaaaagc cggtgatcag aaatatcatt gatgatttga
    11521 aaaagagaaa ctctgtgtcc ggcagaacca tacagatcgc cgacaaaggg ttgaattgtg
    11581 ctgaaaatat cttccatgcg ctcaaaaatg gggatggata tattttctct aagtccgtaa
    11641 agatgcttcc ggaaacggaa aagacctggg tactgctccc taataattac agggatgtaa
    11701 aaaatgctgc cggggagact ttgtatcgca ttaaggaatg tgtagatgaa tttgaatata
    11761 aatttaccga gtctgaaaca ggctctttaa aaaagttccg gatcacagaa aaaaggattg
    11821 ttactttcaa tccaaaactt gccaaaaaac agatctacga aattaataaa gaagttgaaa
    11881 aagcaaggct tctgaaagct tcgcaggcta aaaaatccga gtatggtgat tctgcaaaat
    11941 atgtcatttt tactgctgca gataaaaagg gcaacgacac agatggtaaa gtaaaagtca
    12001 ccatgaatga ggaactcatt caaaaatccc tggagcttgc cggatacaac atgctggtca
    12061 cttccgagat ttccatgaaa gataaggatg tctacaatgc ataccacaat ctctggagaa
    12121 tagaagaatc cttccggatc atgaagtcac agctggatgc cagaccggta tacttgcaaa
    12181 aacaggatac cattgttgga cattttttaa tctgctatct ggccgtatta ctgacaagat
    12241 tactgcagtt caagatatta aaaaatggtt atagttccga agagctgttt gaattcgtac
    12301 gtgacttcag agttgccaaa atatcagaca gaaaatatat aaatctatcg catggtacat
    12361 catttatcag ggaattgtca gagctgtgca gcttaccact gacatcgtat tttttgaacg
    12421 aaggagacat caaaaaaatg ctaagccaca ggttttaatc cttggcttag caccatgttt
    12481 tatacttcaa ctccgaaagt caggttttat aaattaatac tccaaaagca gaaagtcaag
    12541 atgttttttt gtatttgtct aatttttgtt gattttttta aatactgtcc tctcattgac
    12601 gttgctattg acaaatgata caatgtcata gacttgaaaa aacaggaggc cccccctttt
    12661 atgaattctc ttgacaaacg actgcagatg tccggcttca tcactttttt tctaagcggt
    12721 atctgctcca tcagcagcgg tattgtagta agcctgctgc aggaacgata tggatttgac
    12781 tacaatgtaa caggcacact tttatctatt atgagcatcg gaaatctgat ggctggtttt
    12841 gccacaggag tccttcctgc aaagatcgga cttaaagcca ccattattac actaagcgca
    12901 ggttatgaac tgggatatct gtggctaggg atttccggct ggatgagcat tctggtgctt
    12961 tctttccttc tgatcggctt tgccaaggga agcgttatca atatgtgtac catcctggta
    13021 agcaaccatt ctgaaaaccg tgcaaaaggc atgaacatca tgcattcctg ttatgcctta
    13081 ggagctctga tctgcccctt tgtaattgcc ggggccggac tgatcggacc tgtagttccc
    13141 atgtttactt taggcttttt aggacttatc atgtggatca ttttccatct gacaccattt
    13201 gaaaagaaaa cagctggaaa aaaagaaaaa acagactggg gatttttaaa aagcaaacgt
    13261 ttctggctcc ttacaggact tatcttctgc cagaacgcag cagagaccag cgtaaacggc
    13321 tggctggtaa cttactttaa gggaagcggc attttatccg gtactttaag cgcctacacc
    13381 gtgactgtta tgtggactgc cacactgatc ggaagacttc tgattgcctt tgtattccct
    13441 cttaaaaatc catacacagc catgatcaaa atgggcattg gctgtaccgt attttacatt
    13501 ggactgatga tggcaaacaa ccaggtagct gctattctcc tgcttttcgc ctttgctttt
    13561 tccatggccg gcatgaaccc aactgccgtt gcctgtgccg gaaagatgac cagcgtagcc
    13621 agcatcggtg ttttactccc ggctgccagc tttggcggca tcctgatgcc atggatcatt
    13681 ggtattgtct ccaacattgc gggactgaaa atgggtatgg caatgaatat tatttcctgt
    13741 gtgggaatgc tgatattctg tatcgctgta aaaaacttaa acgaagaata ataaatattt
    13801 tccccgtctt tgaccggcgg ggattttttg aactttcata acgtcctcat tttcctgtgt
    13861 ttctttggta tttcaagaca cgaaaaagac cggtcgggga accggtcttt ctcaggagaa
    13921 ggtatttcgt ttcttatggg gtgtagcaaa aactatataa tgtattgggg ggttacgcct
    13981 attataatag cataggatcc tcaaaaagtg tgcacaaagt ttcgattttt gtcttttttc
    14041 ttttatgcat tttatccaac aactgtttct gacttttctt ctttctggcg tttaggccac
    14101 ttttcctccg gctgctggct ttttttataa aagtactgga taatgtcttt tcgtgtaata
    14161 ataccgataa aacatttctg gtcatctaat acaggcacaa aattctggtt taacgctttt
    14221 ccgatcaggt cttccatatc tgcgtctgcc tttaccggac ggttgtccag tctgcgcttg
    14281 atggctgtaa tggggatctt ctccgcacct ttcagatcca gatggtactg atttttgatg
    14341 ccccaaagca gatctccttc tgtcagagtt ccgatatagc ggcctgttct gctgataatg
    14401 gggatggcag aatatttgtg gtactccatc ttttccaaag cctgtcttaa ggtatcttcc
    14461 tcactaatat aagctacttc gcttttagga gtcaggaaaa ataaaatgtt catctcgatg
    14521 ctccttttgt tattattcta aaaggattat accttaaggc tctgaagctt cagtgaataa
    14581 tttctaacat ttttcttaaa ttgttgatct gatctctttt atctgatctt tactccatct
    14641 gatccagtgg atccagtgtt ttttcctgat gttttaattc aaaaaataca ttcacacctt
    14701 ctacggaata atattttgta ggttctgcca cgtatcctaa gatctgccct gcttccagat
    14761 actcacctgg aacagcggaa atttccttta gctgcccaca ggtagcagtg tactcatttc
    14821 ccagatccag cacaacaaag ttgccgatct cttcattgga acctacttcc agcacctttg
    14881 catttgcggg ggctgccacc ggatcactga cctctgcctg gatcacaatg cccggattgc
    14941 atttaaactg gtccagagtg gggaaataaa tcgtctggtc catactgtaa tccaggatca
    15001 cattcccccg caccggccag gtaaggacac tggtatctga aaaatttaat accagggctt
    15061 tggctgcctg ttttcctgca cctgcttctg ctgcaagatc atccttggtg tttggatttt
    15121 tctcttcttt ttgtgcgtaa ttttcctgtg gtacctttga cgcttcgatc tcagaaattt
    15181 tttcttctgc cttttcttcc ttctgtgcag tttcaggaac actgtcctgt gcgataattt
    15241 ctcctcccgg cacttccaga taaggactct gtcccttttc atctgtgttg ctgaccgtaa
    15301 gtacacctac tgctgctacc gccgtaagca ggccgatcac catcatcacc agaaaaagct
    15361 tatctttgaa catctgattt actttatctt tcacctttta tcacctcggt agtattctta
    15421 ccatttcagt gtgctttatt cagtgaaaaa tgcaataccg ctgtaaaaat attccagtat
    15481 ttctttaaaa tcccagccat cttccgcttt tgcctctgct tcattctggc tcagtccata
    15541 gccatgtccc tttcctttta ctgtaatgcc gatcccgttt tcctggggtt ctgtaaaaaa
    15601 gcagggggag gaaagtccaa atgcaccggc tgcttcctct ccagtaaagg aacattcatc
    15661 aatctggatc tcctgtacat acccggctgt atctcgtttt acagtctgca ctttccccgg
    15721 aaatgcttcc gggcggacct gtctcacttc cccttctttt cccgggattt cattcagggc
    15781 tgctgccgcc tgtttggcag ataaagatac aaaactgata aaatccgctg ctttttcatc
    15841 atcgggacag gaaactgact tcagatacgg atatccctca tccccatccc tggtagaacc
    15901 ggcggtgacg gcgcaaaaaa gaggagcgat catctcccca tcatatgtca tcacaattcc
    15961 attggtctgg atcactgcct cttccagacg gctgtatttt tccggaaatt ccgtttttcc
    16021 ccatattttt ttcagctctt ccctcggttt ccggtccaga tccagactta attccgaaat
    16081 ttctttttct tctcccatct gcctgcggat ataagtcctt gcgatcactg cctgggcttt
    16141 gagcgcttcc atttcactgt ccggcgagat ctgagctgcc agtaccgatg gcaggtattc
    16201 ttccaacgac aggtaaccgc cattttcctc ccggtccaga aggatcctgt attttcccgc
    16261 agcttctttt tcctgctgcc ttacatattc tgccccgtgt atatttcccg ttactgccag
    16321 taccgtccca taggggatca ggaaaccggc tgcaaccagt acgaccatcc atttttccat
    16381 aaaaaatccc tgcctcccat atatttatca tcatcataaa tatatggaga cagggcacta
    16441 aactatcctt ttttatcagc caatataagc ctggatcagg cggaaggtgt tttctgcccc
    16501 atctctgtgg cttcgttcat agccatggga agcatacaca ccaggaccaa tgagagcatg
    16561 gcggatatca tttcctgcgc gaagtgtagt ttccacatct gaaccataat gaggatatac
    16621 gtccactgca aagccaatgt tattctcctt tgccagctgg accagatggc ttacgatctc
    16681 ataattataa ggtccgccgc tgtcttttgc gcagatagaa acttcccgct ctgtacacat
    16741 caggccttct cctacacagc ccatatccac agaccaggct tctgtcacgc catccggcac
    16801 agaagcactg cctccatgtc ccacttcctc ataaatggta aaatgagcat aaacagcacg
    16861 ctgcggctct atcttctcgt cttttaaata ttttccatat gcaagtaaaa tggcagctga
    16921 aagtttatca tccagaaaac ggctttttat ataaccggag gatgtaactc ttgtccttgg
    16981 ttcaaagcac acataatctc ctggcataat ccccagtttt aaagcatctt ctttagagct
    17041 taccggctcg tctataagaa cctctactgt atcccagttt cgttcagttt tttcataatc
    17101 tccgtttaca tgaacggacg cattcactaa ctggcataca ccttcataaa taccgtctgc
    17161 ctttgttatg atacgcacat tttccgcctc tgcattagcc ggtttcatac ctccgatatt
    17221 ggtaagacgg agacgggcat ttgccttaat ttccgctacc atgcctccca gtgtatcgca
    17281 atgtgcctcc aacagaatac catcttcttt gttctttccg ccaaaagaag ctacaacacc
    17341 accttttacg gtgcgtttcg cctggatccc cagacgatct aattcctcta acagataatc
    17401 tgttacctgg cggcaatagc cggaaggact gtcaatggac aataattttt ctgcctgttc
    17461 tatgatgtaa tctgtatatt ttctgtagtt tgtcatgtat tatttttcct ctgttttctg
    17521 tttatgtcct ctcggagtct ttcatgcacc tgcctttgtg gctgattttc cgatcatacg
    17581 ctccgctccg gacatcttct gtctcttcct cttaacttat aataagcatt tttttctgca
    17641 tacatcctct gttctgccgc ttttaacata gactcaagca tgccttcttt tttccattcc
    17701 agtcccatgg aataactgat atgtgcctgc tttaaatctt cctgtacctg ctccatctgt
    17761 tcctcaaatt ctgcctttcc tgactctgga agaatgatca caaattcatc tcctccaatg
    17821 cggtaggaat tttccggaaa tgttcttaag atttcttttg cagcatcaca gagcagccgg
    17881 tcccctgctt catggcccag attatcattg atctgtttta aaccatttaa gtcaatataa
    17941 gcaacacctg tgtcgcatac aggatatttt tcaaaagttt ccagtacttt catgtactta
    18001 ttgcggttat agagccctgt aagcatatca cgaaaactta aaaactgcaa acgttcctgc
    18061 tgtctctggg aagactggct gtttacaata aaatactgga gagataataa taaggtcgca
    18121 tcatcaaaat gaacctttgg attgtccaca ccaataaaac ctgtgacttt accgtttttc
    18181 tttaaaggaa ccgttaaaag gcgcttgata ttctgttcca ctaacatttc gtaggtggga
    18241 tatccctttt cctgttctgc atctgcagta taataaacct gaccattttc aaacttatcc
    18301 atccacactg caattacact gagaggaacc gactggagat tgtcgatctg acgggtcacg
    18361 ccttctgctg tatattcgta tgtattgatc acgatctgtt tttcatgatc aatatcaaaa
    18421 atataacttc tgtctgcctg gaaatactgg ttgatgatcc ctaaaagatt atagatggct
    18481 gtatggatat cacggtccgc agataattcc accgcacact tgtttaaagt agtagctact
    18541 tccaactgat ggatcctctc tttatttgac ttctccatcc gtgcttttaa actgccaacg
    18601 atcaggatat tggcaatgat cccaaaaatg actaccatta tcttatcacg attggaagca
    18661 tcaaaataga aaaaactgtg gtccgttgaa aagaaataca tagaataaat gtatgtgata
    18721 aacccacaga gcataccggc tccgtatcca aactgtacca gaaccgcgga caggatcaca
    18781 aacagaataa tattgggatt ggaaatgtca aatttattca tgatcacaca ggccagtacc
    18841 tgcaaaatcc ccgtaatt
//
LOCUS       DBMDUA010000034        18639 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1786_length_18639_cov_3.254288, whole genome shotgun sequence.
ACCESSION   DBMDUA010000034 DBMDUA010000000
VERSION     DBMDUA010000034.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 18639)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18639)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18639
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1786_length_18639_cov_3.254288"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="ACZPCL_04420"
     rRNA            <1..150
                     /locus_tag="ACZPCL_04420"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            674..880
                     /gene="rpmE"
                     /locus_tag="ACZPCL_04425"
     CDS             674..880
                     /gene="rpmE"
                     /locus_tag="ACZPCL_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR1161762.1"
                     /translation="MREGIHPNYYQAKVVCNCGNEFVTGSTKKDIHVEVCSKCHSFYT
                     GQQKAAAARGRIDKFNRKYGINAQ"
     gene            956..1912
                     /locus_tag="ACZPCL_04430"
     CDS             956..1912
                     /locus_tag="ACZPCL_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR1161763.1"
                     /translation="MKYSGIGGQAVIEGIMMQNGENYAVAVRKPDGEIEVKKDTYRSV
                     TKKYPFLGIPFIRGVFKFADSMIVGMRALTWSCSFFEDDEDAKESRFEQWLDKVFGEK
                     LESILMTVVMIFSFIMAIGIFMLLPMFISNICKKVIPSHTVMAILEGFIRIAIFIIYI
                     KMVSRMEDIKRTFMYHGSEHKCINCIEHGLELNVANVRASSKEHKRCGTSFIMIVMVI
                     SILFFMVIRTDTIWLRIVSRIVLIPVIAGVSYEFLSFAGKHDSKLVDILSRPGMWMQG
                     LTTKEPDDTMIEVAIAAVEAVFDWRAYLDENFPGWEKKEEQA"
     gene            1915..2805
                     /gene="prmC"
                     /locus_tag="ACZPCL_04435"
     CDS             1915..2805
                     /gene="prmC"
                     /locus_tag="ACZPCL_04435"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR1161764.1"
                     /translation="MTLGTLLEEGKEKLEKAGVPDPLLDARFLLLDVFDMNFASFLVK
                     RDRPLTGAENGINGTDADPDTLKKIEKYRELISKREMRIPLQQLTGVQEFMGLEFQVN
                     EHVLIPRQDTETLVELVLSEHKEKNISLLDVCTGSGCIAISLAKLGGYTDVTALDLSE
                     EALKVARSNGEKLLDQPVKFIKSDMFSALNPEKQYNVIVSNPPYIPSQVIEGLEPEVK
                     DHEPRMALDGEPDGLKFYRILAEEGKKYLKNGGSIYMEIGWDQAKDVTEIFEAMGFSG
                     LRTVRDMAGNDRVVCARKEK"
     gene            2811..3884
                     /gene="prfA"
                     /locus_tag="ACZPCL_04440"
     CDS             2811..3884
                     /gene="prfA"
                     /locus_tag="ACZPCL_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR1161765.1"
                     /translation="MFDKLDDMLIHYEELMRMLGDPDVTQDTKRFTKLMKEQAELAPI
                     VEAYKQYKQAKQDVEDSLAMLEEENDEEMREMAKEELSDAKKRIEELEHELKILLLPK
                     DPNDDKNIILEIRAGAGGDEAALFAAELYRMYSNYADSQHWKVEIISINENGIGGFKE
                     VVAMVTGKGAYSKLKYESGVHRVQRVPETESGGRIHTSTATVAVMPEAEEVDVQIDMN
                     DCRIDVMRASGNGGQCVNTTDSAVRLTHIPTGIVIYSQTEKSQLQNKEKAFRLLRSKL
                     YDLELEKRQNSEAEERRSQIGTGDRSEKIRTYNFPQGRVTDHRIKLTLYKIDSIMNGD
                     IQELLDNLIAADQAAKLAKMNEN"
     gene            complement(3997..5031)
                     /locus_tag="ACZPCL_04445"
     CDS             complement(3997..5031)
                     /locus_tag="ACZPCL_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1161766.1"
                     /translation="MPTLKDVAALAGVSAATVSLYLNGKSAGRISQQKQKEIEEALKQ
                     LSYQPSSASRQLKELGTSPLIYTVAVYWAADPRSALLGKVMAGIQSSLAKNQMTNCNI
                     VICPFKPNELYKEKGLIDPKLYNYDAAIIANTAVMDMQYLDSIMPPIPVVLLNRRSDQ
                     YSYVSINNQKMGASLADMIADRGFKSVSVFRSQSPYLAVDDRVTGFINGCRAKGVDMP
                     NHALFYTDNSTDGGIQAVAEFLKLSERPDVVFCDSDSIALGALYAFYQAGVRIPKDLS
                     VISIGLHHSSTSKCSIPPLTVSEVPLTQMAEGCMDYVIRALTDKSYAADPEPVHVELE
                     TKIIVRESFQ"
     gene            5369..6736
                     /locus_tag="ACZPCL_04450"
     CDS             5369..6736
                     /locus_tag="ACZPCL_04450"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HIR1161767.1"
                     /translation="MFFWKTAKETVPERPIRISSVEWRDGQQSLLATRIRTQDMLPIL
                     PKMDEVGYECMEMWGGATFDVAIRYLGDDPWERVREFKKLCKKTPLRMLLRGQNLIGY
                     KQYPDDVVEKFVELAAKAGIDKFLVFDGLNDVRNTETAIKAVLKNGKTAEANICYTIS
                     PVHTLEKYVQMAKDYAALGVKVIHVEDMAGMMNPKQVSDVIRAIRKEVGLPVHFHAHC
                     IGGMADICYWEAIKAGACTVDCDVSALSLGPAHPPVESIVTCLKGTGFDPHLDMDLLA
                     EINDYFMKLREKYKEFASKRTGVDISVLKHQIPGGMLSNLESQLKGMNAENRINEVFE
                     EVHKVHKDFGYPPLATPFAQMVGAQATINVLTGQRYKMISNESKTYIRGMYGRPAGEI
                     APELKAAVLGNDKMITERPGSLIAPGWEKAKAEAAGFARTEEDVMTYALFPELAPAFL
                     QKKYQ"
     gene            6805..7188
                     /locus_tag="ACZPCL_04455"
     CDS             6805..7188
                     /locus_tag="ACZPCL_04455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014664.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR1161768.1"
                     /translation="MKQENKEKIAQAEEILERLKEQRGGNVLSIHKKMANDPKLLQAF
                     SQQFAICKQDITHVPAKYMELMLMLMGCCAGNAVTIKTHGELAVKKGATMDEVGEVLR
                     LVFFYYGASAIIPAVELFEELEPEK"
     gene            7320..10856
                     /gene="nifJ"
                     /locus_tag="ACZPCL_04460"
     CDS             7320..10856
                     /gene="nifJ"
                     /locus_tag="ACZPCL_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR1161769.1"
                     /translation="MEEKQKKLMKTMDGNEAAAYVSYAFTEVATIYPITPSSPMAEHV
                     DAWSAAGKKNLFGQRVKLVEMQSEAGACGAMHGALESGALATSYTASQGLLLMIPPMY
                     RISGQLHPGVLHVSARSVGTHAFSIFGEHSDVMATRQTGFAMLCSSTVQEIMDLAGVA
                     HLAAIKGRVPFLHFFDGFRTSHEIQKVEVMDYADLDRLLDRDALDAFRKRSLNPERPV
                     QRSTVQNPDVYFQYREAANPYYEAIPEIVENYMGEINKITGRDYHLFNYYGCPDAEEV
                     IIAMGSVDGVIRETVDYLNAHGRKTGYLQVHLYRPFSIKHFLSALPETVKHIAVMDRT
                     KEPGSLGEPLYLDVCSAIAESGRNIRVYAGRYGLSIKDVTANQIEAVFNNLEAEEPKN
                     HFTVGINDDVTHHSLAVGAPIDVVAEGTISCKFWGLGSDGTVGANKNSIKIIGDHTDM
                     YVQAYFEYDTKKSGGVTRSHLRFGHKPIRGSYLVSHADFVACHNQSYIQKYDIVQECK
                     PGGTFLLNCIWNEDELDKHLPASVKRYIAENHIKFYIINAVKIGKEIGLGNRTNAVLQ
                     SAFFKLANVIPFDDAVEYMKKAILKSYGKKGEKVVNMNYQAVDCGYQRLVEVKVPEEW
                     ASLEPETAKVDENLPEFVKNLMLPINSLKGDSLPVSAFTGREDGTVPLGTSKYEKRGT
                     AVDVPEWDPNKCLQCNQCSYVCPHAAVRPFLVSEAEAENAPEGFKTKKATGKGLEEYT
                     YRIQVSPLDCLGCGACVQVCPAKEKALTMKPLDSQAAEIENWNYAVKLSKKKNPLAKN
                     TVKGSQFETPLLEFSGACAGCGETPYAKLMTQMFGDRMVIANATGCTQAWGAALPCVP
                     YTTNEKGFGPAFSNSLFENNAEFSLGMCLAVTQQRERVAGVVKELKENGVDASLSVAI
                     DAWFDAYDDGDKTMEVSENLVKALEAAELTGEQADRKADILNNKEHLSKKSMWMYGGD
                     GWAYDIGFGGLDHVLASGTDVNILVVDTEVYSNTGGQASKATQIGAVAQFAASGKKTK
                     KKDLGLMAMQYGYCYVAQVAMGANPAQLAKVLKEAEEFKGPSLIIAYAPCINHGLVKG
                     MGCAQQEAKEAVAAGYWNLYHYNPDLKKEGKNPFVLDSKEPTLNLRDFLMGEVRFSAL
                     TRTFPEEAEKLLAEAELDAKEKYQEYKKLAEG"
     gene            10877..11458
                     /locus_tag="ACZPCL_04465"
                     /pseudo
     CDS             10877..11458
                     /locus_tag="ACZPCL_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012550.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
     gene            12005..12496
                     /locus_tag="ACZPCL_04470"
                     /pseudo
     CDS             12005..12496
                     /locus_tag="ACZPCL_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009533275.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LeuD/DmdB family oxidoreductase small subunit"
     gene            12512..13765
                     /locus_tag="ACZPCL_04475"
     CDS             12512..13765
                     /locus_tag="ACZPCL_04475"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR1161770.1"
                     /translation="MGHTLAEKIIMKHIGGKSVKPGDLVVVEPDCVVIHDIYTAFLQD
                     KMKKMGLTKVWNTDKIVIMHDHLMPACLEGDPRSLEAGYELVKEYGIRHFHATGGIVH
                     QLVPELGYSKPGDIVFVTDSHTPTYGAVGCFSTGVGYTEMAAVWGTGQMWLRVPSSIK
                     IQIDGVLPPHVYAKDIILRVLGDLGAAGGTYKSLEFCGTAIDALGIDGRMTIANMVVE
                     CGGKAGLFAADQKCADYCGVDYEDVKWVKADEDAEYERVLTYKAEDLEPVLSCPPYVD
                     NVHPLSEVKGVRLDQVFIGSCTNGRLEDLKIAANVMKGKKISSHVKCIVTPASNSIME
                     EAMKEGYIQTLVEAGALVTPPYCSFCEGRTMGLLSDDEVVLGTNNRNFLGRYGSPKAK
                     AYLSSPEVAAASAVMGVITDPAELS"
     gene            13804..14715
                     /locus_tag="ACZPCL_04480"
     CDS             13804..14715
                     /locus_tag="ACZPCL_04480"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HIR1161771.1"
                     /translation="MRSLREVRDSLLAREKKRIAVAAAEDRPVLEAVKEAVQMGIAEA
                     TLCGDEAKIRQIAEEIELDVNKVTIVNEPDIRKSAEAAVKLVHNGEAQLVMKGLIGTA
                     DFLRAVLNKEWGLRTGKILSHVAVFDSVKLDRLILMTDAAMVMYPTLEEKIAMINNTI
                     PVCQALEIEDPKAAAVCAVEVLNPSMQPTLDAASLAIMSQRGQFKGITVDGPLALDNA
                     LNEDAARHKGIKSPVAGHADVLLMPNIECGNIMWKTLVYMARCEIAGVLMGAAAPVVI
                     TSRSDAPETKLNSIALALLIAGNKDKK"
     gene            14728..15795
                     /gene="buk"
                     /locus_tag="ACZPCL_04485"
     CDS             14728..15795
                     /gene="buk"
                     /locus_tag="ACZPCL_04485"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002834.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HIR1161772.1"
                     /translation="MKDKVLILNPGSTSTKIAVYCNDEIIHLESIQHSHEDLAPFAHV
                     QDQKEYRLEKILNVLDKAGIKLDELICISARGGLLHPIPGGTYEVNDIMVDEMMHPKK
                     EHACNLGALIGKDLADRVSIKSYIVDPVVVDELWPWARMTGLKEVKRVCQFHVLNQKA
                     IARKVARVLGKTYEEVNVIVCHIGGGITVGMHRCGKVVDVNNGLDGDGPMSPERSGGL
                     PNADVLDLAYNHGLTYEEAYRTMVGKGGFVSHLGTNDARAVEAMIDNGDEYAKLVFET
                     MAYQIAKEVGALATTVNGRVDAIALTGGIVYSKRMADWITDRVSFIAPVYRYPGEGEI
                     QALYEGAMRVVRGEEEAKIYE"
     gene            15853..17184
                     /locus_tag="ACZPCL_04490"
     CDS             15853..17184
                     /locus_tag="ACZPCL_04490"
                     /inference="COORDINATES: protein motif:HMM:NF015355.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HIR1161773.1"
                     /translation="MGKKVEFKVCGLSAKYFVPLFLVIIICTYGGFMPTVKIGETTGV
                     GMVGTLAFLMSVGGLFFWLGNSIPVVNSYLGGSVLFCMFGPALLNYFGLLPETTVKGV
                     SILMKYGLQDVYIAALLCGSVLYMDRKILLGAISRYLPAILGSQVFALGFCFLGGLIT
                     GYGPLNAVFDIGAPTMSGGSGGALVTIPTLYTDLSGTDWMSMSGMFLCYVSLSNVVAI
                     LMCALSKPILKKMGMVSPEDNQGILRGAGTMQTEASEQLPALSADPTKVCGGIFICLG
                     IYLLGTILGKLPGTKVIAGLAWAIIIAVVIKCTGIMEDKITNYTVNGMNFMLKGLLPA
                     IIAGIGINSISIETMISYFTPAALVIIVLAVLGAYIGAIIFGHLFGLWGYEAGITAGL
                     CCCNIGGSGDLAVLTAGDRMSLLAFSSISTRIGGALMVVWIGFLYPMLMLK"
     gene            17431..18519
                     /gene="leuB"
                     /locus_tag="ACZPCL_04495"
     CDS             17431..18519
                     /gene="leuB"
                     /locus_tag="ACZPCL_04495"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR1161774.1"
                     /translation="MEINIAVIPGDGIGPEITEATEQVLKRVCEIYGHVLNITHVKAC
                     SSSIEETGEPLPKESLKKCLQADAVLLGNTGLEKYKNEPLTKRPEYALLKLRKELGVT
                     TNLRPVRLYPALMELSPLNKNTLSGGLDYLFVRDIAGGVLCSEQVKGKGVHGPEAYEY
                     EYYNQKIVEDTARFAFELAKGRRNKVVSLDKANVLGSSRLWRKTVTEIGKEYPEVELS
                     HEYIDSAALKLIRRPWEYDVIFTANLFGDIISDEGTGLTGTAGLFGSAELSVSGKGLY
                     TPNQLHYPDESLIGKQKVSPVGMIMAAALMLRCSFGLEKEAKTVEEAVEKVLEAKIST
                     EDMKYPGAKIVLTGEMADFCCQFLGKDV"
BASE COUNT         5665 a         3709 c         4672 g         4593 t
ORIGIN      
        1 aaggcatcta agcgtgaagc ccccctcaag atgagatatc tcatacgcaa gtagtaagac
       61 cccttgaaga cgacgaggta gataggtcag aggtggaagt gcggtaacgc atggagctga
      121 ctgatactaa tcggtcgagg gcttgaccaa taaggtctga gagagcggcg gaaggatgac
      181 agaggagacc gttgaaagtt tactttcata aggtcggatc tggaagactt cagacattgg
      241 cgaagccaat gacatgagga aaatcgaaga tttttcgaat gaccaccgcg gagttggata
      301 acaaaggttg taaagtattc ggaagatcaa cagttcaata tgtggttttg aaggtatgta
      361 aaatatatat ttagcctgag cagaaatgct caggtttttt tgtgtataaa ttcaatattt
      421 tctcttgtca atgtggataa actatgctat actgatatag ctgtccagag atacaatata
      481 tttatgccct gagacaagca gttttagcat tcgcgtgagt cagaaccttg cgggaaggat
      541 gcgtataact taaaagatgc tgatatctgt gcgttctggt gacggtacct accggctgcc
      601 tgcagtgtac agaggcagat tggcatacag gccgcggtcg gaaaccgcaa attatatcag
      661 agaggtgaat aaaatgagag aaggaatcca tccaaattac taccaggcaa aggtagtttg
      721 caactgtggc aacgagttcg taactggttc taccaagaag gacatccatg tggaggtttg
      781 ctccaagtgc cattcattct acactggcca gcagaaggct gctgcagctc gtggacgtat
      841 tgataagttc aatcgtaagt acggcatcaa tgctcagtaa ttataagaga gaatttaagg
      901 ggttgagctt ttgcagctca gcccctttgc gttaaaattg aaaggtaatg catagatgaa
      961 atattcagga attggcggac aggcagtgat cgaaggcatt atgatgcaga atggtgaaaa
     1021 ctatgcagta gccgtccgta aacccgatgg agagatcgag gttaaaaaag atacttacag
     1081 aagcgtaaca aaaaagtatc catttttagg tattcccttt atcagaggcg tgtttaaatt
     1141 tgcagattcc atgatcgtag gcatgagggc acttacctgg tcctgcagct tctttgaaga
     1201 tgacgaagat gcaaaagaaa gccgctttga acagtggctg gataaggtat ttggcgagaa
     1261 attagaaagc atccttatga ccgttgttat gatcttttcc tttatcatgg cgatcggtat
     1321 attcatgctg ctgcctatgt ttatttctaa tatatgtaaa aaagtgatcc cgtcccatac
     1381 agtgatggcc atcttagaag gatttatccg tattgccatt ttcattatct atataaaaat
     1441 ggtatcccgt atggaagaca ttaaacggac ctttatgtac catggttccg aacacaaatg
     1501 cattaactgc attgagcatg gtctggaact gaatgtagcc aatgtacggg ccagctccaa
     1561 ggagcacaag cgttgtggca ccagctttat tatgatcgtt atggtcatca gtatcctgtt
     1621 tttcatggtg atccgtaccg ataccatctg gctgcgcatc gtaagccgta tcgtgctgat
     1681 cccggtgatc gcaggcgttt cttatgagtt tttaagcttt gcaggaaaac atgattccaa
     1741 actggtagat attttaagcc gtcctggaat gtggatgcag ggactgacca ctaaagagcc
     1801 ggacgatacc atgatcgagg tagccatcgc tgctgtagag gcagtatttg actggcgggc
     1861 gtatctggat gaaaatttcc caggctggga gaagaaagag gaacaggcat gaaaatgacc
     1921 cttgggactc ttttggaaga aggaaaagaa aagctggaaa aagcaggtgt tcctgaccca
     1981 cttttagatg cacgttttct tttattggat gtatttgaca tgaattttgc ctcctttctt
     2041 gtaaaacggg accgtcccct tacaggagcg gaaaatggaa taaatggaac agatgcagat
     2101 ccggatacac tgaaaaaaat agaaaaatac cgggaactga taagcaaacg ggaaatgcgt
     2161 atccctcttc agcagcttac aggtgtccag gaatttatgg gactggaatt tcaggtaaat
     2221 gaacacgttc tgatcccaag gcaggataca gaaactctgg tagaactggt attaagcgaa
     2281 cacaaagaga aaaatatttc ccttttagat gtatgcaccg gttccggctg cattgctatc
     2341 agtctggcaa agcttggggg ctatacagac gtaacagctc ttgatctgtc agaagaagcg
     2401 ttaaaggtgg caagatccaa tggggaaaaa cttctggatc agccagtgaa atttataaaa
     2461 agtgatatgt tttctgcttt gaatccagaa aagcagtata atgtgatcgt atccaatccc
     2521 ccctatatcc ccagtcaggt gatcgaggga ctggagccgg aggtaaaaga tcatgagcca
     2581 cggatggcct tagacggtga gccggacgga ctgaaatttt accgcatttt agcagaagaa
     2641 ggaaaaaaat acctgaaaaa cggcggaagc atctatatgg agatcggctg ggatcaggca
     2701 aaggatgtaa cagaaatatt tgaagcaatg ggatttagtg ggcttaggac tgtccgggat
     2761 atggctggaa acgaccgggt ggtatgtgcc aggaaggaga agtaaaaatt atgtttgata
     2821 aactggatga tatgttgatc cattatgaag aactgatgcg tatgcttggg gacccggatg
     2881 tgacccagga taccaaacgt tttaccaagc tgatgaagga gcaggctgaa ctggcaccga
     2941 tcgtagaagc ttacaagcag tataagcagg caaagcagga cgtagaagac agtctggcaa
     3001 tgttagagga agaaaatgac gaggaaatgc gtgagatggc aaaggaagaa ttatccgatg
     3061 caaagaagcg cattgaggaa ctggagcatg aattaaagat ccttcttctt ccaaaggatc
     3121 cgaacgatga caagaacatt atcctggaga tccgtgcagg cgctggcggt gacgaggcag
     3181 ctcttttcgc agctgaactt taccgtatgt attccaacta cgcagacagc cagcactgga
     3241 aagtagagat catcagtatc aatgaaaatg gtattggcgg ctttaaggaa gttgtggcta
     3301 tggtcacagg aaagggcgct tattccaagt taaaatatga gagcggtgta catcgtgtac
     3361 agcgtgtgcc tgagacagag tccggcggcc gtatccatac ctctacagca accgtagcag
     3421 ttatgccgga agcagaagaa gtagatgttc agatcgatat gaacgactgc cgcattgacg
     3481 taatgcgtgc gtctggtaac ggtggacagt gtgtcaatac cactgactcc gcagtacgtc
     3541 tgacccatat accgactggt atcgttatct acagccagac agaaaaatca cagctgcaga
     3601 acaaggaaaa agcattccgt ctgttacgtt ccaaactgta tgatttagag ctggaaaaac
     3661 gccagaactc cgaggcagaa gaacgccgca gccagatcgg taccggagac cgttccgaga
     3721 agatccgtac ctacaatttc ccacagggcc gtgttaccga ccacaggatc aagctgacct
     3781 tatacaagat cgattctatt atgaacggtg atatccagga actgttagac aacctgatcg
     3841 ccgcagatca ggctgcaaaa ctggctaaga tgaatgagaa ctaagattta atcagtcagg
     3901 tatattaaaa tcagatactt agatacagta taaagaaagc tattgcaaag tgatgatgtg
     3961 aagtcaattt gcaatagctt ttttgtttac ggcattttac tgaaacgact ccctcacaat
     4021 gatcttcgtt tccagttcta catgtaccgg ctctggatct gcagcatagg atttatctgt
     4081 caaagcgcgg atcacatagt ccatacagcc ttctgccatc tgtgtcagcg gaacttcgga
     4141 tacagttaat ggcggaatgg agcatttact ggtggatgag tgatggagtc cgatgcttat
     4201 gacggacagg tcttttggga tacggacgcc ggcctggtag aaagcgtaga gtgctcctaa
     4261 ggcaatggag tcggagtcac agaagacaac atccggacgt tcacttaact tgagaaattc
     4321 tgcaacagcc tggataccgc cgtctgtaga attatctgtg taaaataagg catgatttgg
     4381 catgtccact cctttggcac ggcagccatt gataaaaccg gtaacacggt catccacagc
     4441 caggtaagga gactgggaac ggaaaacaga cacgctttta aagccccggt ctgcgatcat
     4501 atcagcaaga gaggcaccca ttttctggtt gttgatggaa acatagctgt actgatcaga
     4561 acgtctgttt aaaaggacca cggggatagg cggcataata ctgtctaagt actgcatgtc
     4621 cattacggca gtatttgcga tgatagcagc atcgtagttg tacagtttcg gatcgatcag
     4681 tcccttttct ttgtacagtt catttggctt aaatgggcag atcacaatat tgcagttggt
     4741 catctggttt ttggcaagag atgactggat cccggccata acttttccta aaagtgcaga
     4801 tcgtgggtca gctgcccagt atacagcaac tgtgtatata aggggggaag tgcccagctc
     4861 ttttagctgc ctggaggcag atgagggctg gtaacttaac tgttttaaag cttcttctat
     4921 ttctttttgt ttctgctggg agatccttcc cgcagatttt ccgttcaaat ataaggatac
     4981 agtagcggct gaaacgcctg ccagcgcagc tacatctttt aacgtaggca taaaaaacct
     5041 cttttctggt ctgttctttt tattctgtat ttatcatatc atacgcccca tataaaagca
     5101 attaaaaaga cataagaagt taacaaatta acttaagtta acaagtaaca ttaaaatatc
     5161 aactaaatca aaacacaaaa actgtgcaaa atatccaaaa aacaaaaaaa taagcattta
     5221 ctattgctat ttaatgagaa aagatgtata gtttgctcag aaacaagtta ataacttaac
     5281 taaacatcga ataaattaaa ttaacatatt aacctaaaga gaatgaaaac cagtgaaaac
     5341 acataaaaca tgtaaatgga ggtgcagaat gtttttctgg aaaacagcaa aagaaacagt
     5401 tcctgaaaga ccgatcagga tcagcagtgt agagtggcgt gacggccagc agtccctttt
     5461 agcaaccagg atccgcacac aggatatgct cccgatcctg ccgaagatgg atgaggttgg
     5521 atatgaatgt atggaaatgt ggggcggtgc aacttttgat gttgctatcc gctatttagg
     5581 agatgatccc tgggaacggg taagggaatt taagaagctg tgtaagaaga caccgctgag
     5641 aatgctcctc cgtggacaga acctgatcgg ctacaaacag tatcccgatg atgtggtaga
     5701 aaaattcgta gaactggcag caaaagccgg aattgataag ttcctggtat ttgatggttt
     5761 aaacgatgtg agaaatacag agacagccat taaggcagtg cttaaaaacg gaaaaacagc
     5821 agaagcaaat atctgctaca ccataagccc ggtacatacg ttagaaaaat atgtacagat
     5881 ggcaaaggat tatgcagcat tgggcgtaaa agtcatccat gtagaagata tggccggaat
     5941 gatgaacccc aagcaggtat cggatgtgat cagagcgatc cgcaaagaag taggtcttcc
     6001 ggttcatttc catgcacact gtatcggcgg tatggcggat atctgttatt gggaagccat
     6061 taaagccggt gcctgtaccg tagattgcga tgtatctgct ctttctcttg gccctgccca
     6121 tccgccggtt gaaagtattg ttacctgctt aaagggaaca ggctttgatc ctcatctgga
     6181 tatggacctt ctggcagaga tcaatgatta tttcatgaag ctgcgtgaaa aatacaaaga
     6241 atttgcaagc aagcgtacag gtgtagatat cagtgtttta aaacatcaga tcccaggcgg
     6301 aatgctttct aacctggaaa gccagttaaa aggcatgaat gcagaaaacc gcattaatga
     6361 agtatttgag gaagtccata aggtacataa agatttcggt tatccgcctt tagcaactcc
     6421 gtttgctcag atggtaggcg cccaggcaac tattaacgtc cttacgggac agagatacaa
     6481 gatgatctcc aatgagagca aaacttacat ccgcggcatg tacggacgtc cggctggtga
     6541 gatcgcaccg gaactgaagg cagctgtttt aggaaatgac aaaatgatca cagaacgtcc
     6601 gggaagcctg atcgctcctg gatgggagaa agcaaaagca gaagcagcag gctttgcaag
     6661 aacagaagaa gatgttatga catacgctct gtttcctgaa cttgccccgg catttttaca
     6721 gaagaagtat cagtaaaggt aatgttttcg tacccgcatt ccgtgggtac agaaaggaac
     6781 agacaatcaa aaggagtaca taccatgaaa caggaaaata aagaaaagat cgcccaggca
     6841 gaagagatcc tggaacggtt aaaggagcag agaggcggaa acgttctttc tatccacaaa
     6901 aaaatggcaa atgatccaaa gctgctccag gcattcagcc agcagttcgc gatctgcaaa
     6961 caggatataa cacatgttcc ggctaaatac atggaactga tgttaatgct tatgggatgc
     7021 tgcgcaggaa atgctgtgac gattaaaaca cacggcgaac tggcagtgaa aaaaggtgcc
     7081 actatggatg aggtcggcga agttttaaga ctggttttct tttactatgg tgcatcagca
     7141 attataccgg cagtagaact ttttgaagaa cttgagccgg aaaagtaaca cccttaaaac
     7201 agtgagtaca gaaatattac catacagaat gccatggtag aaacgtattt agaaatattg
     7261 ccaagtagat ttaaaaaccc cataccataa aggcattcac gaatctcagg aggaaaatga
     7321 tggaagagaa acagaaaaaa ttgatgaaaa caatggatgg taatgaagcc gctgcttatg
     7381 tgtcttatgc atttacagaa gtagctacca tttaccccat taccccttct tcaccaatgg
     7441 cagagcatgt agatgcctgg tctgcagcag gtaagaaaaa cctgtttgga cagagagtca
     7501 aactggtaga aatgcagtct gaggctggcg cctgcggcgc tatgcacggc gcactggagt
     7561 ccggcgcact ggcaaccagc tacacagcat cccagggcct gctccttatg atcccgccaa
     7621 tgtaccgtat ttccggtcag ttacacccag gtgtccttca tgtaagtgcc cgttccgtag
     7681 gaacccacgc attctccatt ttcggagagc actctgatgt aatggcaacc agacagaccg
     7741 gttttgcaat gctttgtagc agtacggttc aggagatcat ggatctggct ggcgttgccc
     7801 atctggcagc tatcaagggc cgtgttcctt tccttcattt cttcgatggt ttccgtactt
     7861 cccatgagat ccagaaagta gaagtaatgg attatgcaga tttagacaga ctcttagaca
     7921 gagatgcact ggatgcattc cgcaagagat ccttaaatcc ggagcgtcct gtacagagaa
     7981 gtaccgtaca gaacccggac gtatacttcc agtaccgcga agcagcaaac ccatattatg
     8041 aagcaattcc tgagatcgtt gaaaactaca tgggagagat caacaagatc acaggaagag
     8101 attaccacct gtttaactac tacggatgtc cggatgcaga agaagtgatc attgcaatgg
     8161 gatctgtaga cggtgtgatc cgtgagacgg ttgattactt aaatgcacat ggcagaaaga
     8221 ccggatattt acaggtacat ctgtaccgcc cattctccat taagcatttc ctttctgcac
     8281 ttcctgagac tgtaaagcat attgcagtta tggaccgtac caaggagcca ggttccttag
     8341 gagaacctct gtatctggat gtttgcagcg ctattgcaga gtctggcaga aatatccgcg
     8401 tatacgcagg cagatatggt ctttctatta aagatgttac tgcaaaccag atcgaagcag
     8461 tatttaacaa cctggaagca gaagagccaa agaaccactt tacagtaggt atcaatgatg
     8521 atgtgaccca tcattcactg gctgtagggg caccgatcga tgtagttgca gagggaacca
     8581 tcagctgcaa gttctgggga cttggttcag acggtacagt tggtgccaac aagaactcca
     8641 ttaagatcat tggtgaccac acggatatgt atgttcaggc atattttgag tatgatacca
     8701 agaaatccgg cggtgttacc cgttcccatc ttcgtttcgg acataagccg atccgtggaa
     8761 gctatctggt atcccatgca gactttgttg cctgccacaa tcagtcttat atccagaaat
     8821 atgacattgt ccaggaatgt aaaccaggcg gtactttctt attaaactgc atctggaatg
     8881 aagatgaact ggacaagcat ctgccagcat ctgtaaagcg ctacattgca gaaaaccata
     8941 ttaaatttta catcatcaat gcagtaaaga tcggtaagga gatcggactt ggaaatcgta
     9001 ccaacgcagt tttacagtca gctttcttca aactggcaaa tgtgatcccg tttgatgatg
     9061 cagtagagta catgaagaaa gccatcctta agtcctacgg aaagaagggc gaaaaggttg
     9121 taaatatgaa ctatcaggca gttgactgcg gataccagag actggtagaa gtaaaggttc
     9181 cggaagaatg ggcttcttta gagccggaga cagcaaaggt agatgaaaat cttccagaat
     9241 ttgtaaaaaa tcttatgctg ccgattaact ccttaaaggg tgacagcctt ccagtcagtg
     9301 catttacagg cagagaagat ggtaccgtac ctcttggtac ttctaaatat gaaaagagag
     9361 gaactgcagt agatgttccg gaatgggatc cgaacaagtg tctccagtgt aaccagtgtt
     9421 cctatgtgtg cccacatgca gcagtgcgtc cattcctggt aagtgaggca gaggcagaaa
     9481 atgcaccaga aggatttaag acaaagaagg caactggaaa aggcttagag gaatacacct
     9541 acaggatcca ggtttctcca ttagactgct taggctgcgg tgcctgcgtt caggtatgcc
     9601 cggctaagga aaaggctctt accatgaagc cattagactc ccaggcagca gagatcgaaa
     9661 actggaatta tgcagtaaaa ctttccaaga agaaaaatcc actggctaag aataccgtta
     9721 agggaagtca gtttgagact ccattattag agttctccgg cgcatgtgca ggctgcggtg
     9781 aaacacctta cgcaaagctg atgacccaga tgtttggtga ccgtatggtg atcgcaaatg
     9841 caaccggctg tacacaggca tggggcgcag cacttccttg tgtaccttat acaaccaatg
     9901 aaaagggctt tggaccggca ttctccaact ccttatttga gaacaacgca gaattttccc
     9961 ttggtatgtg ccttgcagtg acacagcaga gagaaagagt tgcaggtgtt gtaaaagagc
    10021 tgaaggaaaa tggcgtagat gcatctttaa gtgtagcaat tgatgcatgg tttgatgctt
    10081 atgatgatgg cgacaagacc atggaagtaa gcgaaaacct ggttaaggca ttagaagcag
    10141 cagaacttac aggagagcag gctgaccgca aggcagatat cttaaacaac aaggagcatc
    10201 tgtccaagaa atccatgtgg atgtacggcg gcgacggctg ggcttatgat atcggctttg
    10261 gcggtttgga ccatgtatta gcaagcggaa ctgacgtaaa catcctggtt gtagataccg
    10321 aagtttactc caatacaggc ggtcaggctt ccaaggcaac acagatcggt gcagttgcac
    10381 agtttgcagc ttccggtaag aagacaaaga agaaagattt aggcctgatg gcaatgcagt
    10441 atggttactg ctatgtagcc caggtagcta tgggtgcaaa tcctgcacag ctggcaaagg
    10501 tattaaaaga agcagaagaa ttcaagggtc cgtccctgat catcgcctat gctccatgta
    10561 tcaaccacgg tcttgtaaag ggcatgggat gcgcacagca ggaagcaaaa gaagcagtag
    10621 cagcaggtta ctggaacctg taccactata atccagacct taagaaagaa ggaaaaaatc
    10681 catttgttct ggactccaag gagccaactc tgaacttaag agacttcctt atgggtgaag
    10741 taagattttc cgcattaaca agaaccttcc cggaggaagc agaaaaactt cttgcagaag
    10801 cagaactgga tgcaaaggag aagtatcagg agtataaaaa actggcagag gggtaataaa
    10861 aatgggaaat gtgaagatgc agaaagtgga tctgtgtgtg atccaggata atgaacttgc
    10921 acaggtattt ttaaaaacag aaaagaaacc cgcagtagca accgtagttg taattgatca
    10981 gaccgtacct cccaattcac cggaggtatc cggtctgcat aaagccttaa gagaaaaggc
    11041 ggaaaaggaa aacattccat gttactatgg aaaaggaatg tgcggaaagc ttcttgcaga
    11101 tggtctttta aaggccggtg aaagagccgt gacctgtgat aaggcagcgg gagcagccgg
    11161 cgccatcggc gctcaggtca cctgcgcaga tccgacacag ttaaagcagg tattagaaac
    11221 cggcgaactg ctgttagaag aacctgaaaa cggtgcttta aaagaaacag taaaattaac
    11281 tggaaaactg ctagagggac tttctgcaaa agatctggct ttaagcttgg taaaagaact
    11341 gggagcagaa aatttagcag gaaagctttt aagcctgaca ggagatacct tagaggatat
    11401 ggaactgtct gaaaaaatgg ttctctgcgg agttcttgaa aaagccggag caactgctgt
    11461 tacagtggaa aaagaggcag aggaaaagac agatgttgta tttgatgcag cacagacaaa
    11521 agaactgact gcttttccag gaggatataa agagtttgca gcattagagg aactgccggt
    11581 gacagatgta aaatccgtat ttatcggcgg aactaccggc ggcaatttaa aagatctgga
    11641 aaccgtatct tctatgttaa agggaagaaa gatcgcatac ggactgcgcc tggtagtagc
    11701 accttctgat gcagacactt atatcaaggc agcaactgcc ggatatatta cagatatttt
    11761 agaggctggc ggaattgtga tcaaccactg cggaaatccg gaagttcagg gacgtattgg
    11821 aaaaacggaa gttatggtat ctaatgatct ggaaaataag cctggttatg ccggctttga
    11881 cacatcaaag acctatctga cttccacaaa agcagctgta gaagcagccc ttcttggaac
    11941 cttaggttat aaagaagaag caccggcagc agaggaagaa gagacagaaa atcattctct
    12001 tacatttgaa ggacgctgct ggaagtttgg aaacgatatt gatacagata ttatcatccc
    12061 aacacagcac ttaaactatg aaaccatgga tgagatcaag gttcatgcat ttgagccttt
    12121 aagaccggaa ctggcagcta aacttcgtga aggtgatctg atcgtagcag gaaataactt
    12181 tggctgtggt tcttccagag aacaggctgc agaggttctg gtagcaaatg gtgtacactg
    12241 tgttattgca aaatcatttg cacgtatttt cttccggaat gctattaata acggtatcct
    12301 gcttctggaa tgcccggatc ttccggataa tgtaaatgag ggagatacag taaaagtaga
    12361 gctggataag cagcagatca gtgccaacgg aaagacttac aagatcccgg caattccgga
    12421 aaatctgtac cacatcatca tggacggtgg tctggtaaag agcattatga aaaaagtaga
    12481 gaaaggcctg ttataggaag ggggtaagaa tatgggacat acattagctg aaaagatcat
    12541 tatgaagcac attggaggta aaagcgtaaa gccgggagat ctggtagttg tagagccgga
    12601 ctgcgtagtg atccatgata tttataccgc tttccttcag gataaaatga agaaaatggg
    12661 acttaccaag gtgtggaata cagataagat cgtcatcatg cacgatcatc tgatgccagc
    12721 ctgcttagag ggcgacccac gaagcctgga agccggttat gaactggtaa aagagtatgg
    12781 tatccgccat ttccacgcta ccggcggtat cgttcaccag ttagttcctg agttaggcta
    12841 ttccaagcca ggtgacattg tatttgtaac agactcccac acacctacct acggtgctgt
    12901 aggctgcttc tctaccggtg ttggatacac agaaatggca gctgtatggg gaaccggcca
    12961 gatgtggtta agagttccaa gcagcattaa gatccagatc gacggagtac ttcctcctca
    13021 tgtatacgca aaggacatta tccttcgggt attaggagat ctgggagctg caggcggtac
    13081 ctataagtct ctggaattct gtggaactgc cattgatgca ttaggtattg acggccgtat
    13141 gaccattgca aacatggttg tagagtgcgg tggaaaagca ggacttttcg cagcagacca
    13201 gaaatgcgct gattactgcg gcgtggacta cgaagatgta aaatgggtca aagccgatga
    13261 agatgcagaa tatgaaagag ttctcactta taaggcagaa gatttagagc cagtattatc
    13321 ctgtccgcca tatgtggaca atgtgcatcc actgtctgag gtgaaaggcg tcagactgga
    13381 tcaggtattt atcggttcct gtaccaatgg ccgtctggaa gatctgaaga tcgcagcaaa
    13441 tgtgatgaag ggcaaaaaga tcagctctca cgtaaaatgc atcgtaacac ccgcttccaa
    13501 cagcattatg gaagaggcaa tgaaggaagg ctatatccag acgctggtag aagcaggagc
    13561 gctggtaaca ccgccttact gttccttctg tgaaggacgt accatgggtc tgctttcaga
    13621 tgacgaggta gttcttggaa ccaacaaccg taacttctta ggacgctatg gttctccaaa
    13681 ggcaaaagca tatctgtcat caccggaagt agctgctgca tccgctgtta tgggcgtgat
    13741 cacagatccg gcagaactga gttaaaacag ataggatcat aaattataga aaggacctgg
    13801 aaaatgagat cattaagaga agtaagagac agcctgttag ccagagagaa aaaaaggatc
    13861 gcagtggctg cggcagagga cagacctgtt ctggaagcag taaaagaggc ggtacagatg
    13921 gggatcgcag aggcaactct ctgcggtgat gaggcaaaga tccgccagat cgcagaagag
    13981 atcgagctgg atgtaaacaa agtaaccatt gtaaatgaac cggatatcag aaaatcagca
    14041 gaagcggctg taaaactggt acataacggg gaagcacagc ttgttatgaa aggtctcatc
    14101 ggaaccgcgg atttcctgcg ggctgtatta aataaagaat ggggacttcg taccggcaag
    14161 atcttaagcc atgtggctgt atttgactct gtaaaattag accgtctgat ccttatgaca
    14221 gacgcagcta tggtcatgta tccaacactg gaagaaaaga tcgccatgat caacaatacc
    14281 attcctgtat gccaggcact ggagatcgaa gatccaaagg cagcagctgt gtgtgcagta
    14341 gaggtattaa atccttccat gcagcctaca ctggatgcag cttccctggc catcatgagc
    14401 cagagaggcc agtttaaggg catcactgta gacggacctc tggcactgga caatgcatta
    14461 aatgaagatg cagcccgtca caaaggcatc aagagcccgg ttgcaggcca tgcagatgta
    14521 cttttaatgc caaatatcga gtgcggaaac attatgtgga agactctggt atacatggca
    14581 agatgtgaga ttgcaggcgt actgatggga gctgcagcac cagttgtcat tacttcccgt
    14641 tctgatgcac ctgaaaccaa gttaaacagt attgcccttg ctctgctgat cgcaggaaac
    14701 aaggacaaga aataaagagg tacctacatg aaagataaag tattgatcct gaatccaggt
    14761 tccacatcca ccaagattgc agtttactgc aatgatgaga ttatccacct ggaaagtatc
    14821 cagcattccc acgaagatct ggctcccttt gcccatgtgc aggaccagaa ggaataccgc
    14881 ctggaaaaga tattaaatgt tcttgataaa gcgggtatca aactggatga actgatctgt
    14941 atttccgcaa gaggcggact tctccacccg atcccaggtg gtacctatga agtaaatgac
    15001 atcatggtag atgaaatgat gcatccgaaa aaggaacatg cctgcaacct gggtgctctt
    15061 attggaaaag acctggctga ccgtgtaagc attaaatcct atatcgtaga cccggtagtc
    15121 gtagatgagc tgtggccatg ggcaagaatg acaggtctta aagaagtaaa aagagtctgc
    15181 cagttccatg tattaaacca gaaagctatc gccagaaagg ttgccagagt ccttggaaag
    15241 acttatgaag aggtaaatgt gatcgtctgc catatcggcg gtggtattac tgttggcatg
    15301 caccgttgtg gcaaggtagt agatgtaaac aatggtcttg acggagacgg accaatgtca
    15361 ccggagcgtt ccggaggact gccaaatgca gatgtgctgg atctggcata caatcatggt
    15421 ttaacctatg aagaagctta ccgtaccatg gtaggaaagg gcggatttgt aagccattta
    15481 ggcaccaatg atgccagagc tgtagaagca atgatcgata acggcgatga gtatgcaaag
    15541 ctggtttttg agaccatggc atatcagatc gcaaaagagg ttggtgctct tgctaccact
    15601 gtaaacggca gagtggatgc aatcgcactg accggcggta tcgtttattc caagcgtatg
    15661 gctgactgga tcacagacag agtatccttt atcgcacctg tataccgtta tccgggagaa
    15721 ggcgagatcc aggctcttta cgaaggcgct atgcgtgtgg taagagggga agaagaagct
    15781 aagatttacg aataagcaaa atactatatg tcccctcttg tttgagggga cgactgtaga
    15841 aagaggaaaa atatgggtaa aaaagtggaa tttaaagtct gcggactttc agcaaagtac
    15901 tttgtaccgt tgtttttagt cattatcatc tgtacttacg gcggttttat gccaactgta
    15961 aaaatcggtg agactactgg tgttggaatg gtaggaactc tggcgttttt aatgagtgta
    16021 ggcggcctgt tcttctggct tggtaacagt attcctgttg taaacagcta tttaggtggt
    16081 tccgtgctct tctgtatgtt tggaccggca ctgttaaatt actttggtct tctgccagag
    16141 accactgtaa aaggtgtttc tatccttatg aaatatggtc tgcaggacgt atatatcgca
    16201 gctctccttt gcggttccgt cctgtatatg gaccgtaaga tcttattagg tgctatttcc
    16261 cgttatctgc cggcgatcct gggctcccag gtatttgcac tgggcttctg cttccttggc
    16321 ggactgatca caggttatgg acctttaaat gcagtatttg atattggcgc accaactatg
    16381 tccggtggtt ccggcggtgc tttagtaacg atccctactc tgtacactga cctttccggt
    16441 acagactgga tgagcatgtc cggtatgttc ttatgctatg tttctctttc taacgtagtt
    16501 gctatcctga tgtgtgctct ttccaaaccg atccttaaaa aaatgggtat ggtatctcct
    16561 gaagataatc agggcatcct tcgtggagca ggcactatgc agacagaggc atctgagcag
    16621 cttccggctc tcagtgctga tcctaccaaa gtctgcggcg gtatctttat ctgccttggc
    16681 atctatctgt taggaactat tttaggcaag cttcctggaa ccaaagtgat cgcaggactt
    16741 gcatgggcga tcatcatcgc ggttgtgatc aaatgtaccg gcatcatgga agacaagatc
    16801 acaaattata ctgtaaacgg tatgaacttt atgttaaagg gactgctgcc ggcgatcatc
    16861 gcaggtatcg gcatcaactc tatcagtatt gagactatga tcagctactt tacaccggct
    16921 gctctggtca ttattgttct ggctgtatta ggcgcttaca ttggcgcgat catcttcgga
    16981 cacctctttg gcctgtgggg ctacgaagct ggtattacag caggtctttg ctgctgcaac
    17041 atcggtggtt ccggtgacct ggcagttctg actgcaggcg acagaatgag cctgttagca
    17101 ttctcctcca tctccacccg tatcggcggt gccctgatgg ttgtatggat cggcttcctg
    17161 tatccaatgt taatgctgaa ataaatgacc gtgtaaaagt gccataataa aatcaaatta
    17221 accttcctaa gttaaagaat aagtcaacga aaaagttaaa tattaatcct gaataagtga
    17281 atgataagtg aataaataat gtgaccgccc agaaccggtc cgacctgtaa tggatcggat
    17341 accggtcctg ggcggttttt gcgattatct tgcgctgcca acggcagcac atgtagccaa
    17401 acatgtttgc agaaaggaaa atggaaaaat atggaaataa atatagcagt aataccgggg
    17461 gacgggatcg gtccggagat cacagaggca actgagcagg tcttaaagag agtgtgtgaa
    17521 atatatggtc atgtacttaa cattactcac gttaaggcct gttcttcttc tatagaagaa
    17581 acaggagaac ctcttccaaa agagtcttta aaaaagtgcc ttcaggcaga tgcagttctt
    17641 ctgggaaata caggtctgga aaaatataaa aatgagcctt tgaccaagcg cccggaatat
    17701 gcccttctta agttacgaaa ggagctggga gttactacca acctgcggcc ggtgcggcta
    17761 tatccggcat tgatggagct ttcaccatta aataaaaata ccctttccgg cgggcttgat
    17821 tatttatttg tccgtgatat agcaggggga gtattatgct cggaacaggt aaaaggaaag
    17881 ggggtacatg gcccggaagc ttatgaatac gaatattata atcagaagat cgtggaagat
    17941 acggcccgtt ttgcctttga actggcaaaa ggaagaagaa ataaggtggt aagtcttgat
    18001 aaggcaaatg tactgggaag ctccagattg tggcggaaaa cagtaacaga gatcggaaaa
    18061 gagtacccgg aggtggaact gagccatgaa tatattgatt ctgcggcctt gaaactgatc
    18121 cgtcgtccct gggaatatga tgtgatcttt actgccaatc tgtttgggga tattatctcc
    18181 gatgaaggaa ccgggttgac tggaactgca ggactttttg gttccgcaga actttccgta
    18241 tccggaaaag gactatatac acctaaccag cttcattatc cagatgagtc attgatcgga
    18301 aaacagaagg taagtcccgt gggaatgatc atggcagcgg ccctgatgct gcggtgcagc
    18361 tttggactgg agaaagaggc aaaaacagta gaagaggcag ttgaaaaagt gctggaagct
    18421 aaaattagta cagaagatat gaaatatccg ggggcaaaaa tagttttaac aggggaaatg
    18481 gcagattttt gttgccaatt tttgggaaaa gatgtatgat ttttaaaaga aaagagttaa
    18541 taactaaaac ttcccacacc gatgtggtgt gttgaacctt gaaaattcaa tatttcagcc
    18601 aataaaaaag gcataaacct gttccgtttg gtataatgg
//
LOCUS       DBMDUA010000035        18314 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1815_length_18314_cov_3.307857, whole genome shotgun sequence.
ACCESSION   DBMDUA010000035 DBMDUA010000000
VERSION     DBMDUA010000035.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 18314)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18314)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18314
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1815_length_18314_cov_3.307857"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="ACZPCL_04500"
     CDS             complement(<1..484)
                     /locus_tag="ACZPCL_04500"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1161775.1"
                     /translation="MSKYTITITREFGSLGRPIAHALSQKLGIEYYDRDIVDEVAKQL
                     NLPVSQISDAEESSHNHFFSHMFPLGTDKEYIQDMIFDVQKDIILDLARKSSCIIVGR
                     CSDFLLQNEENVVNIFIYAPYKARLENCIHSLEMSEDEAKRMIMKVDKARLAYHKKYA
                     G"
     gene            complement(499..1161)
                     /locus_tag="ACZPCL_04505"
     CDS             complement(499..1161)
                     /locus_tag="ACZPCL_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1161776.1"
                     /translation="MLTLFHQIFTPANTGFMLKGLGMTIVIAVLAILISMFFGTILAL
                     IRTYASGRFKWAASLVAVYTEFFRCTPNLLWILWIYFTVKGNKIAVSVFAISLFTSAV
                     MAEIVRGGLNSISKGQFEAAQSQGFSFIQTLWYIILPQTYRKIIPALLSQVITVIKDT
                     SFLKMVDVAEFMRNCAVVMGSIYDVHGMIMLIGFEALCYFVICFALSCIVRSYQKTIV
                     TA"
     gene            complement(1155..1883)
                     /locus_tag="ACZPCL_04510"
     CDS             complement(1155..1883)
                     /locus_tag="ACZPCL_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1161777.1"
                     /translation="MIKGLFDGKRWAALWKAVPDYYVPGFIMTLKISAAGLLTALILG
                     VIFGTFSTMKFKPAKILSRIYVEFIQNTPLALQVLFYYSVLPLLFSNSGFRIPKFVLG
                     VIGVGVYHGAYISEVIRTGIEAIPRGQSEAAASQGFSYLETMYYIILPQTIRIIMPPL
                     ANQALNLVKNTSILAMVAGMDLMYFTDSWGSERNFFAQAYFTSALLYFIICFPLARLA
                     RYLEIRSMRMPAPKKILFEEEGTC"
     gene            complement(1951..2847)
                     /locus_tag="ACZPCL_04515"
     CDS             complement(1951..2847)
                     /locus_tag="ACZPCL_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1161778.1"
                     /translation="MKKRVLSMAMATVMAASLTACGSGSTATTAAPTETNADTSAAAS
                     EAASEAAGSSDADVQKIIDRGVLKVGCKSDVPNFSLQNTATGEYEGFEDDLAYEIAGD
                     IFGCSADEAKEKKLVEFQGVTAKTRGPLLENGEIDLVIATFTITEERKETYNFSTPYY
                     TDAVGLLVNNDSGIESIEDLNGKIIGVAQSSTTKDGFNSYIEEQGLDVKPQFQEFDGY
                     PALAQALATKQIDCFSVDRAILSGYVNDSNHILPDRFCEQEYGVASAKENTGLADLVD
                     KKVTSMLSDGSMKALQDQWGLQ"
     gene            complement(2940..3671)
                     /locus_tag="ACZPCL_04520"
     CDS             complement(2940..3671)
                     /locus_tag="ACZPCL_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1161779.1"
                     /translation="MADMVKVEHLKKNFGDLEVLKDINLTVAEGEKLVIIGPSGSGKS
                     TFIRCINHLEEPTSGTISIAETPVTKKNRLEMAKKYSSMVFQQFNLYPHLTVLENLTL
                     APVKLQHVPKDKARETALKCLDRVGLKAKAGNYPAQLSGGQQQRVAIARALCTNQPLI
                     LFDEPTSALDPEMVQEVLNVMIELAHENITMICVTHEMGFARQVADRVIFMDGGYIIE
                     EGTPEHFFTAPEHDRTKAFLSKILH"
     gene            4017..5513
                     /gene="thrC"
                     /locus_tag="ACZPCL_04525"
     CDS             4017..5513
                     /gene="thrC"
                     /locus_tag="ACZPCL_04525"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR1161780.1"
                     /translation="MALSYESTRGGQKGVTASQAILKGLAEDGGLFMPTEIPKLDKTL
                     EQLAGMTYQETAYEVMKLFLTDYTEEELKSCIANAYDDKFDTEEIAPLAKADGAYFLE
                     LYHGSTIAFKDMALSILPHLMTTAAKKNHADKEIVILTATSGDTGKAAMAGFADVPGT
                     RIIVFYPKGGVSKVQELQMRTQKGDNTSVVAIHGNFDDAQTGVKKLFGNKELEKEMAE
                     AGFQFSSANSINIGRLVPQVVYYVYAYAKLLENEQIENGEEINVVVPTGNFGNILAAY
                     FAKHMGVPIKTLICASNDNKVLYDFFTTGTYDRKREFILTNSPSMDILISSNLERLIY
                     LSTGCDAAADAALMKDLSEKGSYTVTENMRQFMSDFRGGFATEAQNEATIRKIFEDTG
                     YLIDTHTGVAATVYNAYKAETGDQTKTVIASTASPYKFSHSVLEAIKGKSAVEGKDEF
                     AVVDELSEVSGTQVPKAVEELRSAVIRHNTECDVDKMGETVKNILGIA"
     gene            5657..6520
                     /gene="fba"
                     /locus_tag="ACZPCL_04530"
     CDS             5657..6520
                     /gene="fba"
                     /locus_tag="ACZPCL_04530"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HIR1161781.1"
                     /translation="MLVSAKEMLEKARAGKYAVGQFNINNLEWTKSILLTAQELNSPV
                     ILGVSEGAGKYMTGFKTVAAMVSAMIDELNITIPVALHLDHGTYEGCYKCIKAGFSSI
                     MFDGSHYPIEENIEKTKELVKIAHAMGLSLEAEVGSIGGEEDGVVGMGECADPKECKA
                     IADLGIDFLAAGIGNIHGKYPANWQGLNFEVLENVKKEVGDMPLVLHGGTGIPADMIK
                     KAISLGVAKINVNTECQLSFAAATRKYVEEGKDLQGKGFDPRKLLAPGAEAIKATVKE
                     KMELFGSVGKA"
     gene            6780..8894
                     /locus_tag="ACZPCL_04535"
     CDS             6780..8894
                     /locus_tag="ACZPCL_04535"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIR1161782.1"
                     /translation="MSEVVSVSEMFGKDVFNDAVMKDRLPKSVYKKLKKTIEDGAELD
                     PSIADVVAHAMKDWAIEHGATHFTHWFQPLTGVTAEKHDSFISAPVDGKVIMEFSGKE
                     LIKGEPDASSFPSGGLRATFEARGYTVWDCTSPAFLKEDAIGVTLCIPTSFCSYKGEA
                     LDKKTPLLRSMQAVNEQALRILRLFGNTTAKRVVPSVGPEQEYFIVDRAKYLQRKDLI
                     YTGRTLFGAMPPKGQEMDDHYFGVIRERIGSYMNDLNKELWKLGVPAKTQHNEVAPAQ
                     HELAPIYEGVNQSVDHNQIVMETMKKVAERHGLACLLHEKPFAGVNGSGKHNNWSLTA
                     DDGTNLMNPGDTPHENIQFLLVLACIIKAVDIHADLLRESASNVGNDHRLGANEAPPA
                     IISIFLGEQLEDVIDQLCDTGEATHSKQGGTLKTGVDILPDFDKDATDRNRTSPFAFT
                     GNKFEFRMVGSSDSIASANTVLNTIIAEAFKEAADKLEKADNFDMAVHDMIKETLAAH
                     KRVVFNGNGYSDEWVAEAERRGLPNIKSMVDAIPALITDKAVKLFEEFGVFTKTELES
                     RVEVEYEGYAKAINIEAKTMIDMAGKQIIPAVIKYTTALAASISAVKSACPEADVSTQ
                     TELLLETSDLLAETKLALAKLTEITEKSAAMDGGKDQAVAYHEEVVPAMAALRTPVDK
                     LEMLVDKDLWPMPSYGDLIFEV"
     gene            9170..11053
                     /locus_tag="ACZPCL_04540"
     CDS             9170..11053
                     /locus_tag="ACZPCL_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR1161783.1"
                     /translation="MNRQALVCRNILEKPDITQREMAKNMDISLGTVNGLVKECLAEG
                     YIAEEENGKEKYLILLEKGKELLKPYKVDGALIIAAGFGSRFVPLTFETPKGLLEVFG
                     ERMIERQIKQLHEVGIHDITIAVGYLKEKFEYLIDKYDVKLLYNPEYSCKNTLATVYR
                     ARKFLKGRNVYILSSDNWMRENMYHSYECGAWYSAAHEEGETKEWCLTFNKKGRISDV
                     NVGGKDAWFMYGPVYLSREFSAKFLPVLEAYYQIPGTEQFYWEQPYVDMLKGEAKRRL
                     EKEDKELLKQTEEACGIPASKWNEIEMNINRQPDNQVYEFENLEELRLFDTHYQNHSD
                     NAAMELVAEVFHVPEFQITDIKCLKSGMTNKSFLFRTGDHHCICRIPGPGTELLINRE
                     QEKEVYDAVRPLGITEHVLYLNPKTGYKISEYYENTHNASADNWDDMKTCMDMVRKLH
                     EAGLKVGHSFNIRERISFYEKLCLEHGGIPFEDYSQVREWVNQLLDALDKMDLPQCLC
                     HIDANVDNFLIFPDHSAKLLDWEYAGMCDPLMDVSMCAIYSYYEEEQTEKLLELYLQR
                     RPDDKERFITYADAALGGFLWCLWAVYKASLGDEFGEYTIIMYRYAKRYYKKLMKELP
                     QFF"
     gene            11224..12276
                     /locus_tag="ACZPCL_04545"
     CDS             11224..12276
                     /locus_tag="ACZPCL_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR1161784.1"
                     /translation="MGRKKYFAALAAAGMIGAMALGSAFNSYAADGWTKSNDHWTYIY
                     QGKTYTGWLETSDGKYYMDLSDGHMTTGFKQIDGKWYFFRPNGLMAQSRWINPEYGKW
                     YYFLGDGTMVTGWLKIGNDYYFMRGDGSMGTGWRQMDGAWYYFQSSGKCVVNSWAQIK
                     DNWYLFGTDGKMMTGWAKVDTDYYYLNTDGRMLTGWLTDSEGNKYYMNQSNGKMSVGW
                     KQIDNAWYYFNTNGHMMTGWIQVDNKIYYMNPTNGGKMTTGTSLDINGTTYNFDGSGV
                     CQNASGVSAQTPSSSSTSASGGVTGENGGPGVSRNSSSNNGPSGGNTSNNTSNSPSNT
                     TSSSDTLTAGRTDGPG"
     gene            12352..14292
                     /locus_tag="ACZPCL_04550"
     CDS             12352..14292
                     /locus_tag="ACZPCL_04550"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HIR1161785.1"
                     /translation="MNNADKIIKRCRALALAGMIGITSLGSSLPAFAAGPGDDSSRVS
                     EISTGGPGDPGNTNSSNSTTNTGNSNNSNSNNNTNNSNNTNNTNNSNTSSNNTTTNTN
                     AWKKVNGTYQMSDGSAIQNVLHRGIDVSRWQGEINWSQVAKDDISFVMLGTRSKGAVD
                     PYFHKNIQNATANGVKVGVYIYSLATTTDMAVQEADFVLNLITDYPISYPVAFDMEDS
                     TQGNLSKSELAAIANAFCKRISAAGYYPIIYANENWLNNKLDMSQMNYPVWVARYSAR
                     YTWQNPVMWQATSSGSVKGINGNVDIDFQFKDFTSVIPANTWRTINGKRYYYANYEKQ
                     KNAWAQDVNDWYYMDKDGLASTGWITVADARYYLDTTTGKMQTGWQSENGKWYYLGSS
                     GAVKKGWISDNGTWYYTGNDGAMQTGWIEVSGKRYYLENSGKMVTGWTNQNGKWYYLG
                     SSGDLLKGWINDGGTWYYSNNEGVMQTGWLTDAGGKYYLRGSGAMAIGWRQLDGAWYY
                     FNSDGRMATGMIEVGGLRYYMDPSDGRMVSGRAVEINGVTYQAAADGHLSQQGQETTG
                     DQSSQNSGNNASGSNTGNNNTGNNVQITPANSTTGSSDNSTSNSSSDDQKVTGVNGGP
                     GVKKDSANNGNNDKVVTGQAPG"
     gene            14403..15098
                     /locus_tag="ACZPCL_04555"
     CDS             14403..15098
                     /locus_tag="ACZPCL_04555"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR1161786.1"
                     /translation="MHNRYIKYFVFLLFTADLVLSAIFLKNDHRDIEKIFSLPALSKV
                     AGQPGYIALTFDDGPDRKCTPLLLDGLKERGVHATFFLMGSNIEGKEDIVKRMSEEGH
                     LIGNHSYEHIQLTKAGAEAVCEAVEHTQEQIEAITGKRPEYIRPPYGDWNEELEEEIG
                     MSPVLWSVDSLDWKLKDKGKIVRRVLKDVKDGDIILMHDIFPSSVEAALELIDILQKE
                     GYVFVTADELLIE"
     gene            15125..16441
                     /locus_tag="ACZPCL_04560"
     CDS             15125..16441
                     /locus_tag="ACZPCL_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR1161787.1"
                     /translation="MDLFDYMRENTMEKESPLASRLRPRTLDEVVGQQHIIGKDKLLY
                     RAIKADKLGSVIFYGPPGTGKTTLAKVIANTTQADFKQINATVAGKKDMEEVVTEAKN
                     NMGMYGRRTILFVDEIHRFNKGQQDYLLPFVEDGTLTLIGATTENPYFEVNGALLSRS
                     RIFELKPLEKDDIKQLIYRAVTDSERGMGTYRVKIEEDAADFLADTANGDARAALNAV
                     ELGVLTTERSEDGLIHIDLAAAQECIQKRAVRYDKDGDNHYDTISAFIKSMRGSDPDA
                     AVYYLARMLYAGEDIKFIARRIMICASEDVGNADPQALNVAVSAALAAERIGLPEAQI
                     ILSQAASYVACAPKSNAAYVAIQNAMENVKTTRTMPVPVHLQDRHYKGAAKLGHGEGY
                     KYAHDYPKHYVKQQYLPDGMEGTVFYEPSDNGYEKQIKAHMKWLKD"
     gene            16629..>18314
                     /gene="rho"
                     /locus_tag="ACZPCL_04565"
     CDS             16629..>18314
                     /gene="rho"
                     /locus_tag="ACZPCL_04565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR1161788.1"
                     /translation="MREKLETRSLAELKEMAKNVGLKGISGLRKAELIDLLCAQEEKS
                     QKNTAETVTVKPHSSRVNAEKTVREKPVQETRSQETHPQENRVQESRVQENRSQENRV
                     QETRPQENRYQEKRSQDNRNNQRNNRTYERFNQTRPQIRTYGNNNYNNNNNSYSTPAA
                     APVQQAVPVSQTYSTQNQTYVQNVDNQERVARPEYRNDAMGLSTQDMAELDSGIEANG
                     ILEVMPDGFGFIRCENFLPGENDVYVAPSQIRRFNLKTGDIIVGNRRVKAATEKFAAL
                     LYIKTVNGYPLSATETRPNFEDLTPIFPNQRLHMENPREKTSVAMRVLDLLAPIGKGQ
                     RGMIVSPPKAGKTTLLKQVAKAITTNHPDMHLMILLIDERPEEVTDIKEAIVGPNVEV
                     IYSTFDELPERHKRVSEMVIERAKRLVEHGRDVIILLDSITRLARAYNLTVAPSGRTL
                     SGGLDPAALHMPKRFFGAARNMREKGSLTILATALVDTGSRMDDVVYEEFKGTGNMEL
                     VLDRKLSEKRIFPAIDILKSGTRRDDLLLTKEEAEAVDIIRKATNSLKPDEAVE"
BASE COUNT         5655 a         3830 c         4323 g         4506 t
ORIGIN      
        1 atcccgcata ctttttgtga taagccagcc gggctttatc tactttcatg atcatccgtt
       61 tggcttcatc ttcactcatt tccagactgt ggatacagtt ttccagcctt gctttataag
      121 gcgcgtagat aaaaatgttc actacatttt cctcattctg aaggagaaaa tcagagcagc
      181 ggccaacaat gatacaactg gactttcttg ccagatccag gataatatcc ttctggacat
      241 caaagatcat atcctgtatg tattccttgt ctgtaccaag gggaaacatg tgggaaaaga
      301 aatgattgtg ggagctttct tccgcatcac tgatctggga taccgggagg tttaactgtt
      361 ttgccacttc atctacaata tccctgtcat aatattcaat tcccagtttc tgggaaaggg
      421 catgggcgat gggacggccc agacttccaa attcccttgt aatggtaatg gtgtatttac
      481 tcatagcatc tggcctcctt aagccgtaac gatggttttc tggtaactgc gtacaataca
      541 gctaagtgca aagcagatca caaagtagca gagagcttca aaaccgatca gcatgatcat
      601 tccatgtacg tcatagatac tgcccataac aaccgcacag ttgcgcatga actctgccac
      661 atctaccatt ttcaggaaag aagtatcttt gatcactgtg atcacctggc ttaaaagagc
      721 cgggatgatc ttgcggtaag tctgaggcag aatgatatac cacaatgtct ggataaagct
      781 aaagccctgt gactgggctg cttcaaactg acctttggaa atggagttta aaccgccgcg
      841 gacaatttca gccataacag cagaagtaaa taaggagatg gcaaaaacag aaacagcgat
      901 cttatttccc tttactgtaa aatagatcca taagatccac agcaggtttg gggtgcatcg
      961 gaaaaattcc gtatatacgg ctaccaggct ggcagcccat ttaaaacggc cggaagcata
     1021 ggtgcggatc aaagccagga tcgttccgaa aaacatactg atcagtatgg ctaatacagc
     1081 aatgacaatg gtcattccca gtccctttaa cataaaacct gtattggcag gtgtaaaaat
     1141 ctggtgaaat aatgtcagca tgtgccttcc tcctcaaaca gtattttttt aggcgccggc
     1201 atacgcatgg aacggatctc cagatatctg gcaagtcgtg ccagcggaaa acagatgata
     1261 aaatataaaa gcgcgctggt aaaataagcc tgtgcgaaga agtttcgttc agaaccccat
     1321 gaatcggtaa aatacatcag atccatacct gccaccatag caagaataga ggtgtttttc
     1381 accaggttta acgcctggtt ggccagaggc ggcatgatga tccggatcgt ctgggggagt
     1441 atgatataat acatggtttc cagataagag aagccctggg aggcagccgc ttctgactgc
     1501 cccctgggaa tggcttcaat accggtacgt attacttctg atatgtaagc tccgtgataa
     1561 acacctacac cgatcacacc aagaacaaat ttaggaatac ggaaaccgga gttggaaaaa
     1621 agtaaaggta atacagagta gtaaaacagt acctgaagag ccagaggcgt attctggata
     1681 aattcaacat agatacgtga caggatcttt gcaggcttga acttcattgt ggaaaaggtt
     1741 ccaaagatca ctcccagaat aagagcggtc agaagacctg ctgcggaaat cttcagggtc
     1801 attataaaac cgggtacata atagtctgga acggctttcc acaaagcggc ccagcgtttt
     1861 ccatcaaaaa gaccttttat catatctgtc atcctctgct tttctgactt atatttaatg
     1921 taccggagta cattttatgc aaagcgcatt ttactgcagt ccccactgat cctgaagggc
     1981 tttcatagat ccatcagaaa gcatggaggt tactttctta tcaaccaggt ctgcaagtcc
     2041 tgtattttcc tttgcggaag caacgccata ttcctgctca cagaaacggt ctggaagaat
     2101 gtggttagaa tcatttacat aaccggaaag gatcgcacgg tctacagaga agcagtcgat
     2161 ctgcttggtt gcaagagcct gtgctaaagc aggatagccg tcaaattcct ggaactgagg
     2221 ttttacatcc agtccctgtt cttctatata agagttaaaa ccgtcctttg tggtagaaga
     2281 ctgtgcaaca ccgataatct ttccattcag gtcttcaata gactcaatgc cggaatcatt
     2341 attaactaaa agtcctacag catctgtgta gtaaggtgtg gagaagttgt aggtctcttt
     2401 tctttcctct gtaatcgtaa aggttgcgat aaccaggtcg atctcaccgt tttccagaag
     2461 aggtcctctg gtctttgctg taacaccctg gaattctacc agcttctttt cttttgcctc
     2521 atcagcggag catccaaaga tatcaccggc gatttcatat gccagatcat cttcaaaacc
     2581 ttcatattca ccggttgcag tattctggag agaaaaatta ggaacatcgg atttgcagcc
     2641 tacctttaat acgccgcggt cgatgatctt ttgtacatca gcatcagaag aacctgctgc
     2701 ttcggatgcg gcttcggagg cagctgcaga agtatctgcg tttgtttctg tcggtgcagc
     2761 agtagttgct gtagaaccgg atccgcatgc agttaaagaa gctgccatta cagttgccat
     2821 tgccatagat aatactcttt ttttcataat cagtgtcctc ctcttatcta caataaatta
     2881 ctactatatt taatccttct cagagagaag aaataaacag ctaatataat aacaaataac
     2941 taatgtagta tcttacttaa aaatgctttt gtacggtcat gctccggtgc tgtaaagaaa
     3001 tgctccggag ttccctcttc tataatatag ccgccgtcca taaatattac ccggtcagcc
     3061 acctgccttg caaagcccat ttcatgggtc acacagatca tggtgatatt ttcgtgcgca
     3121 agctcgatca taacatttaa aacttcctgg accatttccg ggtccagtgc tgaggtaggt
     3181 tcatcaaaaa ggatcagagg ctggttggta cacaaggctc ttgcaatggc cacacgctgc
     3241 tgctgtccgc cggaaagctg ggcaggataa tttccggctt ttgcttttaa accaactctg
     3301 tccagacatt ttaaagcagt ttcacgggct ttgtccttag gcacatgctg aagttttaca
     3361 ggagccagtg tcaggttttc cagaacggtc aggtgtggat acaggttgaa ctgctggaat
     3421 accatggaag aatatttttt tgccatttcc aggcggttct tttttgttac aggcgtttct
     3481 gcaatggaaa tagtgccgga tgtaggctct tccaggtgat tgatgcagcg gataaaggtg
     3541 gacttaccgg aaccggaggg cccgatgatg accagctttt ctccttcagc taccgtcagg
     3601 ttaatatcct ttaaaacctc cagatcaccg aaattctttt taagatgttc tacttttacc
     3661 atatcagcca tatttctcac cgtctgccat cctgtgtgcc ggaacgtgca gcctttctta
     3721 aaaataaaat cttgaaaata aaagccagga ataagaaaaa ggccacagcg cagagagagc
     3781 aattgtcttt cagcaccgtc gccttttttt cttaagtgtc agttcaaatt ttatgttttg
     3841 cattataagc aggtaaaaat caaatgtcaa gtacaaaaaa ctttttttat attgcggcac
     3901 gcattgaaat atgttacact ttttccaaac taaataccag aataaataat aactgtgcaa
     3961 tgtttatttt gttattaaaa aatttatcaa agtaaagtaa gagagggaga aagatcatgg
     4021 cattatccta cgaaagtacc agaggcggac agaaaggcgt tacagcatca caggccattt
     4081 taaagggtct ggcagaagac ggcggtcttt tcatgcctac agagatccca aagctggaca
     4141 agaccttaga gcagcttgcg ggaatgacct atcaggaaac agcctatgaa gtgatgaagc
     4201 tttttctgac agactataca gaggaagaat taaagtcctg tatcgcaaat gcatacgatg
     4261 acaagtttga cacagaggag atcgcacctt tagcaaaagc agacggcgct tatttcctgg
     4321 agctttatca tggaagcacc attgctttca aggacatggc tctttccatc cttcctcatc
     4381 ttatgactac agctgcaaag aagaaccatg cagacaagga gatcgtgatc ttaactgcta
     4441 cctccggcga taccggaaaa gcagctatgg caggttttgc tgatgttcct ggaacacgca
     4501 ttatcgtatt ttatccaaag ggcggtgtca gcaaggtcca ggagctgcag atgcgcaccc
     4561 agaaaggtga caataccagc gtagttgcta tccacggaaa ctttgatgat gcgcagaccg
     4621 gcgtaaagaa gctgttcggt aacaaagaac ttgaaaagga aatggcagag gctggcttcc
     4681 agttctcttc cgcaaactct attaacatcg gacgtctggt tccacaggta gtttattatg
     4741 tatacgcata cgcaaaactt ctggaaaatg agcagatcga aaatggcgag gagatcaacg
     4801 ttgtagttcc aaccggaaac tttggaaaca tccttgcagc ctactttgca aagcatatgg
     4861 gtgtgccgat caagaccctg atctgcgctt ccaatgacaa caaagtcctt tatgacttct
     4921 ttacaacagg aacctatgac agaaagagag aatttatctt aactaattct ccttccatgg
     4981 acatcctgat ctccagtaac ttagagcgtc tgatctactt aagcacaggc tgtgacgcag
     5041 cagcagatgc agctttaatg aaagatctgt ctgaaaaggg aagttatact gtaactgaga
     5101 atatgcgcca gttcatgtct gatttcagag gcggttttgc aactgaggca cagaacgagg
     5161 cgaccatccg taagatcttt gaagataccg gatatcttat tgatacccat acaggtgttg
     5221 ctgctactgt ttacaacgca tacaaggcag aaacaggaga ccagaccaag acagtgatcg
     5281 catccactgc aagcccatac aagttctctc acagcgtttt agaagctatt aaaggtaagt
     5341 ctgctgtaga aggaaaggat gaattcgcag tagtagatga gctgtctgaa gtatccggca
     5401 cacaggttcc aaaggcagta gaagaactgc gcagcgcagt gatccgccac aatacagaat
     5461 gtgatgtgga taaaatgggt gaaactgtaa agaatatcct tggaatcgct taaaacagct
     5521 tccattttag gacaagatag agtataatga attagtacgt taagaatata acaaagttat
     5581 atatttaaca aagacaggtg tccggaaaat ccgggacaca gtgcaaaaat tgcagtatgt
     5641 tcaaggagga ataaaaatgt tagtatcagc aaaagaaatg cttgaaaaag ccagagcagg
     5701 caaatacgca gtaggacagt ttaatatcaa caaccttgag tggaccaaat ccatcctttt
     5761 aacagcacag gaattaaatt cccctgttat cctgggtgta tctgagggcg caggaaaata
     5821 catgaccggt ttcaagaccg tagcagctat ggtatccgct atgatcgatg aactgaatat
     5881 caccattcct gttgcactgc atctggatca tggtacttat gagggctgct ataagtgtat
     5941 caaagcagga ttttcctcca ttatgtttga cggttcccat tatccgatcg aagaaaacat
     6001 cgaaaagacc aaggaactgg taaagatcgc acatgctatg ggtctgtcct tagaggctga
     6061 ggttggctcc attggcggcg aagaagacgg cgtagtaggt atgggtgagt gcgcagatcc
     6121 taaggagtgc aaggcaatcg cagacctggg tatcgatttc ctggcagcag gtatcggcaa
     6181 catccatgga aaatatccgg ctaactggca gggattaaac tttgaagttc tggaaaatgt
     6241 taagaaagaa gttggcgata tgccattagt tcttcacggc ggtaccggaa tcccggctga
     6301 catgatcaaa aaagctatca gccttggcgt tgctaagatc aatgtaaata ctgagtgcca
     6361 gctgtccttt gcagcagcaa ccagaaagta cgttgaagaa ggaaaagact tacagggcaa
     6421 gggctttgac ccaagaaaac tgttagcacc aggcgcagaa gctatcaagg ctactgtaaa
     6481 agaaaaaatg gaactgtttg gctccgttgg caaagcctga aaataagtta gtataataca
     6541 aaatcttttt ttaaaaaacc acttgcaatt ttaaaagaaa tggtttattt taagaaagaa
     6601 cgaaggcgtt ctctatatca ggagagcgcc ttttttacta cctgatgctt taaacttaaa
     6661 aaaattaggt tttcaggtaa agatagtaag ggatgtactg caaatagctg aagaaaagca
     6721 tctcgaatga ggatttatgg aaatcataaa aaaatgatgc aagagaggat ggagaatcta
     6781 tgagcgaagt agtaagtgtt tcagagatgt ttggcaagga cgtatttaat gacgcagtca
     6841 tgaaagaccg cctgccaaag agtgtgtaca aaaaactgaa aaagaccatc gaagatggag
     6901 ctgagttgga tccaagtatt gcagacgttg tggcacatgc catgaaggat tgggccattg
     6961 aacatggtgc aacccatttt acccactggt tccagccatt gaccggagta actgctgaga
     7021 aacatgactc ctttatttca gcaccggtag acggaaaagt gatcatggaa ttctccggaa
     7081 aggaactgat caagggcgag cctgatgcat cttccttccc atccggtggt ttaagagcaa
     7141 ctttcgaggc aagaggttat acagtatggg attgtacttc cccagccttc ttaaaagaag
     7201 atgctatcgg tgttaccctt tgcattccta cttctttctg ctcctataag ggcgaggcac
     7261 tggataagaa gacaccactt cttcgttcta tgcaggcagt aaacgagcag gcactgcgta
     7321 tccttcgtct tttcggaaat accactgcaa agcgcgtagt tccttctgta ggacctgagc
     7381 aggagtattt cattgtagac cgcgcaaaat atttacagcg taaagacctg atctacaccg
     7441 gccgtactct tttcggggca atgcctccaa agggacagga aatggatgac cattacttcg
     7501 gtgttatccg tgagcgtatc ggttcctaca tgaacgactt aaataaggaa ctttggaaat
     7561 taggtgttcc tgcaaaaaca cagcataacg aagtagctcc tgcacagcat gagctggcac
     7621 cgatctatga aggtgtaaat cagtctgtag accataacca gatcgttatg gagaccatga
     7681 agaaagtggc agagcgccac gggttggcat gccttcttca cgagaagcca tttgcaggtg
     7741 tcaatggttc cggtaaacat aacaactggt ccctgacagc agatgacggt accaacctga
     7801 tgaacccggg agatacacct catgagaaca tccagttcct gttagttctg gcatgcatca
     7861 tcaaggcagt agatatccac gcagacctgt taagagaatc tgcttccaat gtaggaaatg
     7921 atcacagact gggagcaaac gaggcaccgc cagcaatcat ctccatcttc ttaggcgagc
     7981 agctggaaga tgttatcgat cagctttgtg atactggtga ggcaactcac tccaagcagg
     8041 gcggaacctt aaaaacaggt gttgatatcc ttcctgactt tgataaagac gcaaccgaca
     8101 gaaacagaac ctccccattt gcatttacag gaaacaagtt cgagttccgt atggtaggtt
     8161 cttccgactc tattgcatct gcaaatacag tattaaatac cattatcgct gaagccttca
     8221 aggaagcagc tgacaagctg gagaaggcag ataactttga tatggctgta catgacatga
     8281 tcaaggaaac cctggcagct cacaagagag ttgtattcaa tggaaatggt tattccgatg
     8341 agtgggtagc agaagctgag agaagaggac ttccaaacat caagtctatg gtagatgcta
     8401 ttccggcact gatcacagac aaggctgtaa agctgtttga agaattcggc gtatttacaa
     8461 agaccgagct ggagtccaga gtagaagttg agtatgaagg ctatgcaaag gctatcaaca
     8521 tcgaagcaaa gaccatgatc gatatggctg gaaagcagat catcccggct gttatcaaat
     8581 acacaactgc tcttgcagct tccatctccg cagtaaagag tgcttgccct gaagcagatg
     8641 taagcaccca gacagagctt ctgttagaaa cctctgatct tctggcagaa accaaactgg
     8701 ctcttgcaaa actgaccgaa attactgaaa agagcgcagc aatggatggc ggcaaggacc
     8761 aggcagttgc ttaccatgag gaagtcgttc ctgcaatggc agcattaaga accccggtag
     8821 acaaactgga aatgctggta gacaaagact tatggccaat gccgagctac ggcgatctga
     8881 tctttgaggt ttaataaatc ttataaaata gaaggttccg cctgcgtcag caggcagata
     8941 agcgcgagag tgcggtgaac ctgagtattg gacgcaatta cataaataaa atgcccttct
     9001 ggtgatatat aacgtatccc ggaagggtgt ttttttcgcg gtatgagtgg cattgtttaa
     9061 cttgcatttt aaacccagtg tgctataatg ttcatatatt tttgaaaaat aataaaaatg
     9121 aacataaata taaacataag cataagcagg aaaaggagtt aaagatcata tgaaccgaca
     9181 ggcgctggta tgcagaaata tactggaaaa accggatatt acacagcggg aaatggcgaa
     9241 aaacatggac atatccctgg gtacagtcaa tgggctggtg aaggaatgtc tggcagaggg
     9301 atatattgca gaggaagaaa atgggaaaga aaaatatctc atccttctgg aaaagggaaa
     9361 agaactgtta aagccttata aagtagatgg ggcgctgatc attgcagccg gtttcggttc
     9421 ccgttttgtg cctcttacct tcgagactcc aaagggtctt ttagaggtct ttggagagcg
     9481 catgatcgag cgtcagatca aacagctcca tgaagtgggg atccacgaca ttaccattgc
     9541 agtaggctat ttaaaagaaa aatttgaata cctgatcgat aaatacgatg tgaaattatt
     9601 atacaatccg gaatactcct gcaaaaacac gctggctact gtatacagag ccagaaagtt
     9661 cttaaaaggc cggaacgtat acattctttc atcagataac tggatgcggg aaaatatgta
     9721 ccattcctat gagtgcggtg cctggtacag cgcagcccac gaagaaggag agaccaagga
     9781 atggtgcctt acctttaata aaaagggccg tatcagcgat gtaaatgtag gcggaaaaga
     9841 tgcgtggttt atgtacggtc ccgtatatct ttccagggag ttttccgcta aattccttcc
     9901 tgtattagag gcttattatc agatccccgg aacagagcag ttttactggg agcagcctta
     9961 tgtggacatg ttaaagggag aggcaaaacg ccgtctggaa aaagaggaca aggagctgtt
    10021 gaaacagaca gaagaagcct gcggcatacc tgcgtccaaa tggaacgaga tcgagatgaa
    10081 tatcaaccgc cagccggata accaggtata cgaatttgaa aatctggaag agctgcgcct
    10141 ctttgatacc cattatcaga accactccga taatgcggcc atggagctgg tagcagaggt
    10201 attccatgtg ccggagtttc agatcacaga catcaaatgc ttaaaatccg gcatgaccaa
    10261 taaatccttc ctgttccgca ccggagatca tcactgtatc tgccgtatcc ccggacctgg
    10321 cacagagctt cttattaaca gagagcagga aaaagaagtt tatgatgccg tccgtcctct
    10381 gggcattaca gaacatgtgt tgtatctgaa cccgaagacc ggttataaga tctctgaata
    10441 ctatgaaaat acccacaatg ccagcgcaga caactgggac gatatgaaga cctgtatgga
    10501 tatggtgcga aagcttcatg aagcaggttt aaaagtaggc cacagcttta atatcaggga
    10561 gcggatcagc ttttacgaga aactttgcct ggaacatggc ggcattcctt ttgaagacta
    10621 cagtcaggtt cgtgaatggg taaatcagtt gttagatgcc ttagataaaa tggatcttcc
    10681 acaatgttta tgccatattg acgcaaatgt ggataacttt ttaattttcc cggatcacag
    10741 tgcaaaactt cttgactggg agtacgcagg catgtgcgac ccgttaatgg acgtatccat
    10801 gtgtgcgatt tattcctatt atgaggaaga gcagaccgaa aaattactgg aactgtattt
    10861 acagcgcaga ccagatgaca aagagcggtt catcacctat gcagacgcag ccttaggggg
    10921 cttcctctgg tgcctgtggg ctgtatacaa ggcaagtctg ggagatgagt ttggagagta
    10981 caccattatt atgtaccggt atgcgaagcg gtactataag aagctgatga aggagttacc
    11041 acagttcttt tagaaacgcc ccatgatatg ggaacataca caaatgcatt tcaaaacagg
    11101 tttcagaaaa ttgtaataaa aatgttaatc atggaaagtt catttgtgct atacttaatt
    11161 aaaagtgaaa aataattgtg cgatcaatga aaaagagacg atacaacaga acaggaggag
    11221 aaaatgggac gtaaaaaata tttcgcagcc cttgcagcag ctggtatgat cggcgctatg
    11281 gcgttgggat ctgcgtttaa ttcatacgcc gcagatggat ggaccaagtc aaatgaccat
    11341 tggacctaca tctaccaggg caagacctat acaggttggc tggaaaccag tgatggcaaa
    11401 tattatatgg acctttccga tggacatatg actaccggat ttaaacagat cgatggaaaa
    11461 tggtacttct tccgtccaaa cggtctgatg gctcaaagcc gctggatcaa tccggaatat
    11521 ggcaaatggt actacttctt aggtgacggt accatggtta ccggctggtt aaagatcgga
    11581 aacgattatt actttatgcg tggtgacgga tctatgggaa ccggctggcg tcagatggac
    11641 ggtgcctggt actacttcca gagcagcggc aaatgtgtag ttaattcctg ggcacagatc
    11701 aaggacaact ggtatctgtt tggaacagac ggaaagatga tgaccggctg ggcaaaagtt
    11761 gacaccgact actattatct gaatacagat gggcgtatgc ttacaggctg gctcactgac
    11821 agcgaaggca ataagtatta tatgaatcag agcaatggca agatgagtgt aggctggaag
    11881 cagatcgata atgcctggta ttatttcaat accaatggtc acatgatgac aggctggatc
    11941 caggtagata ataagatcta ctacatgaat ccgacaaacg gcggaaagat gacaacagga
    12001 acctccctgg atatcaacgg aactacctac aactttgatg gaagtggtgt atgccagaat
    12061 gcaagcggcg tatctgctca gacaccatct agcagcagta cttctgcttc cggtggtgta
    12121 accggtgaaa atggcggacc tggtgtatca agaaattcca gctccaacaa tggtccttcc
    12181 ggtggtaaca cttcaaataa tacatcaaac agtccttcca ataccacatc cagttctgac
    12241 accttaacgg caggacgcac agacggacca ggctgataga aaacttttaa attgcagact
    12301 acataatggg gatgtcagga aaaagacatc ccctttttca ggagaacagg catgaataac
    12361 gcagataaga tcataaaacg atgccgggcg ctggcactgg caggtatgat tgggatcacc
    12421 agtcttggaa gttctcttcc agcctttgct gcaggtccgg gggatgactc atcccgggta
    12481 tcagagattt ccacaggagg tcctggagat ccgggaaata ctaattcttc caattctaca
    12541 acaaacacag gaaattcaaa taattcaaat agtaacaata atactaataa cagcaataat
    12601 acaaacaata caaacaattc caatacatca tccaacaata ctaccacaaa taccaatgcc
    12661 tggaaaaagg taaacggtac gtaccagatg tcagatggaa gcgcgatcca gaatgtgctc
    12721 catcgtggta ttgatgtttc cagatggcag ggagagatca actggagcca ggtggcaaag
    12781 gatgatattt catttgttat gctgggaacc cgttccaagg gagcagtaga tccctatttc
    12841 cacaagaata tccagaacgc aactgccaat ggtgttaaag taggcgttta tatttattct
    12901 ctggcaacta ccacagacat ggcagtccag gaggcagatt ttgtattaaa cctgatcacc
    12961 gactatccga tctcctatcc ggtagcattt gatatggaag actctactca gggaaatctg
    13021 tcaaaatcag agctggcggc tattgcaaac gctttctgca agcggatttc tgcagccggt
    13081 tattacccga tcatctatgc aaatgagaac tggctgaaca ataaactgga tatgagccag
    13141 atgaattatc cagtatgggt ggcaagatat tctgcccgat atacctggca aaatccggtg
    13201 atgtggcagg ctacaagcag cggttctgta aaaggtatca atggcaacgt ggatattgac
    13261 ttccagttta aggactttac ctctgtgatc ccggcaaaca cctggcgcac cattaacgga
    13321 aagcgttatt attacgcaaa ttacgaaaaa cagaaaaatg catgggcaca ggatgtaaat
    13381 gactggtact acatggataa agacggactt gcatctaccg gctggatcac agtagcagat
    13441 gcccgttatt atctggatac tactaccgga aaaatgcaga ctggctggca gtcagaaaac
    13501 ggaaaatggt actaccttgg aagcagcggt gctgtaaaga aaggctggat cagtgataat
    13561 ggtacctggt actatactgg caatgacggt gctatgcaga caggctggat cgaagttagc
    13621 ggcaaacgct actatctgga aaattctggg aaaatggtta ctggctggac gaaccagaac
    13681 ggcaaatggt attaccttgg cagttccgga gatctgttaa aaggctggat caatgatggt
    13741 ggcacctggt attattccaa caacgaaggt gtgatgcaga ctggatggct gacagatgca
    13801 ggtggaaaat attatctgag aggctccggt gccatggcaa ttggctggag acagttagat
    13861 ggtgcatggt attactttaa cagtgatggc aggatggcaa caggaatgat cgaggtaggc
    13921 gggcttcgtt attatatgga tccttccgac ggtcgtatgg tatctggcag agcagtagaa
    13981 ataaatggcg tcacctatca ggcagccgca gatggacatt taagccagca gggtcaggaa
    14041 acaacagggg accagagcag ccagaattcc ggaaataatg cttcaggcag caatacagga
    14101 aataataaca caggaaacaa cgttcagatc actcctgcaa actctaccac aggttcctct
    14161 gacaacagta cttccaactc ctcttctgat gatcagaaag taacaggcgt aaatggaggc
    14221 ccgggagtaa agaaggactc tgcaaataat ggaaacaatg ataaggtagt aacaggtcag
    14281 gcaccaggct gatgaaataa ctatttaaat ccgcttttgg cagaatggga aatctgcttc
    14341 aaaggcggat ttttttgcgt attcccgcat aaaataaaca gataccattc atcaggtgaa
    14401 ccgtgcataa caggtatata aaatatttcg tctttcttct gtttacagca gatctggtct
    14461 tatccgctat tttcctgaaa aatgatcaca gggatataga aaaaatattt tcacttcctg
    14521 cactttcaaa agttgccggc cagcccgggt atatagccct cacttttgat gatggtccgg
    14581 accggaaatg taccccgctt cttttagacg gactgaaaga acggggagtc catgctacct
    14641 tttttcttat gggaagcaat atagaaggaa aagaagatat tgtaaagcgt atgagcgaag
    14701 aaggacatct tattggcaat cacagttatg aacatatcca gctgacaaaa gcgggagcgg
    14761 aggcagtctg tgaagcagta gaacataccc aggaacagat cgaagccatt accggaaagc
    14821 gcccggaata tatacgtccg ccctatggtg actggaacga agaactggaa gaagagattg
    14881 gaatgagccc ggtcctctgg tctgtagact ctttagactg gaaactaaaa gataaaggaa
    14941 agatcgtccg gcgggtcttg aaagatgtaa aagatgggga tattatatta atgcatgata
    15001 tatttccttc ctctgtagag gcagccctgg agctgatcga tatactgcag aaggaaggtt
    15061 atgtttttgt aacggctgat gaattgttga tcgaatgaga tcaaaagaaa aatggagaaa
    15121 ttaaatggat ctatttgatt atatgagaga aaatacgatg gagaaggagt ctcctctggc
    15181 ttccagactt cgtccgcgca cactggatga ggtagtgggg cagcagcata tcattggaaa
    15241 agataagctg ctctaccggg cgatcaaggc ggataaactg ggatctgtga tcttctatgg
    15301 acctccggga acaggaaaaa ccacccttgc caaggtgatc gcaaacacca ctcaggcaga
    15361 ttttaagcag atcaatgcca ctgtggcggg taaaaaagac atggaagaag tggtcacaga
    15421 ggcaaaaaac aatatgggaa tgtatggacg gcggaccatt ctttttgtag atgagatcca
    15481 ccgttttaat aagggccagc aggattatct tcttcctttt gtagaagacg gtacactgac
    15541 actgatcggt gctactacgg aaaatcctta ttttgaggta aacggggcgc ttctttccag
    15601 atcccgtatt tttgagttaa agccactgga gaaagatgac attaaacagc tgatctaccg
    15661 ggcggtaacc gactcagaac gtggaatggg aacttaccgg gtaaagatcg aggaagatgc
    15721 cgcagatttt ctggcagata cagccaatgg ggacgccaga gcagccttaa atgcagtaga
    15781 actgggagtt ctcacgacag aacgcagcga agacggattg atacatattg accttgctgc
    15841 tgcccaggaa tgtatccaga aacgggccgt gcgttatgac aaagatggag acaaccacta
    15901 tgataccatc tctgctttta ttaaaagtat gcggggatct gacccggatg cggccgtgta
    15961 ttatctggca cgtatgctgt atgcaggaga agatattaaa tttattgccc gccgtattat
    16021 gatctgtgct tctgaagacg ttggaaatgc agatccacag gcgttaaatg tagcagtaag
    16081 cgcagctctt gcagcagagc gcattggact tccggaagct cagatcatcc tttcccaggc
    16141 agcttcctat gtggcatgtg cccctaaaag caatgcagcc tatgttgcca tacagaatgc
    16201 catggaaaat gtaaagacca cccgtaccat gccggtgccg gtacatcttc aggaccgtca
    16261 ttacaaaggt gcagcgaaat taggacatgg tgaaggctat aaatatgccc atgattaccc
    16321 gaaacattat gttaagcagc agtatcttcc agatggaatg gagggtacag tattctatga
    16381 accatcagat aatggctatg aaaagcagat aaaagcccat atgaaatggt tgaaagatta
    16441 acaatggcaa tgtataataa aaaatgataa aaataatttt acgtggatcc caaggcagaa
    16501 attctcactc tgcctgtcgg atctgtctat aaaaggaact tcccaaaatc tggaaaaatc
    16561 ccgtatttta caaagacagg caaactgaat aattcagata acagatgcat aatagatagg
    16621 agagtagtat gcgcgaaaaa ttagagacaa ggtcccttgc cgaactgaaa gagatggcaa
    16681 aaaatgtggg tctgaaaggg atcagcggcc ttcgtaaggc agagcttatt gaccttttat
    16741 gtgcccagga agagaaaagt cagaaaaaca cagcagaaac agtaacagtg aaacctcaca
    16801 gcagccgtgt aaatgctgag aaaacagtgc gggagaaacc agtccaggaa acccgcagtc
    16861 aggagaccca cccacaggag aaccgggtgc aggaaagtcg tgtgcaggaa aatcgcagcc
    16921 aggaaaaccg tgtgcaggag acccgcccac aggaaaacag atatcaggaa aagcgttccc
    16981 aggacaaccg gaataatcag agaaataacc gtacatacga aagatttaac cagactcgtc
    17041 cgcagatccg tacatatggg aacaacaatt ataacaataa caacaatagt tacagcactc
    17101 cggcagctgc ccctgttcag caggcggttc ccgtcagcca gacttattct acccagaacc
    17161 agacttatgt ccagaatgtg gacaaccagg agcgtgtggc aagaccggaa tacagaaatg
    17221 acgccatggg actttccaca caggatatgg cggaattaga cagcggcatt gaggcaaacg
    17281 gtatcttaga ggttatgccg gacggtttcg gctttatccg ttgtgaaaac ttccttccag
    17341 gtgaaaatga tgtctatgtt gcaccgtccc agatccgccg tttcaactta aagaccggtg
    17401 atatcattgt gggaaacaga agagtaaaag cagctacaga aaaattcgct gcacttctct
    17461 atattaaaac agtgaacggc tatccattaa gtgctactga gacaagacca aactttgagg
    17521 atctgacccc gatcttccca aatcagcgtc tacacatgga aaatccaaga gagaaaactt
    17581 ctgttgccat gcgggtatta gacctgctgg cacccatcgg taaaggacag cgtggtatga
    17641 tcgtttcccc gccaaaagct ggtaagacca ctctcttaaa acaggtggca aaagccatta
    17701 ccaccaatca tccggacatg catctgatga tccttttgat cgatgagcgt cctgaggaag
    17761 taactgacat caaagaagcc attgtagggc caaatgtaga agttatctat tccacctttg
    17821 atgagcttcc ggagcgccac aaacgtgtat ctgaaatggt tattgaacgt gcaaaacgtc
    17881 tggtagaaca tggaagagat gttatcatcc ttttagacag tatcacccgt ctggcaagag
    17941 cttacaatct gaccgttgcc ccaagcggcc gtaccctttc cggtggtctt gatccggcag
    18001 cactgcatat gccaaagcgt ttcttcggtg ctgcaagaaa tatgcgtgaa aagggcagtc
    18061 tgaccattct tgcaacagca ttggtagata caggaagccg tatggatgat gtggtttacg
    18121 aggaatttaa gggtaccggc aacatggaac tggtgctgga ccgtaaactg tctgaaaaac
    18181 gtatcttccc ggctattgac attttaaaat ccggtaccag aagagatgat ctgctcctta
    18241 caaaagaaga ggcagaggca gtagatatta tccgcaaagc caccaattcc ttaaagccgg
    18301 atgaggcagt agag
//
LOCUS       DBMDUA010000036        18115 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1836_length_18115_cov_3.068447, whole genome shotgun sequence.
ACCESSION   DBMDUA010000036 DBMDUA010000000
VERSION     DBMDUA010000036.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 18115)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18115)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18115
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1836_length_18115_cov_3.068447"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="ACZPCL_04570"
     CDS             complement(<1..131)
                     /locus_tag="ACZPCL_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR1161789.1"
                     /translation="MKKIREFLHKYRHAWLLSYAFIYLPWFMYLERTVTRDYHIMHAS
                     "
     gene            340..1200
                     /locus_tag="ACZPCL_04575"
     CDS             340..1200
                     /locus_tag="ACZPCL_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1161790.1"
                     /translation="MSEARSVLGVICSAYDSFFEAEKKIADYIMEHKEQAVDMTVGEL
                     AKASGTSDATVSRFCRRCGFKGFQNLKLTLAREILEEEHQSQEVTNDIDRTDLEQSLK
                     NILANKIAELTETIKMMDIKNLEVILDKLEHARMVQLAAVGNTIPVALDGAFKLNQLG
                     IPAVAGDIWEAQAAYAFNLGPEDVVLIISNSGSSKRLETLALGAKENGSTLVLITNNR
                     ESPLAQICDYKIITATREKLLTEEFWFSRVTATTVIEIIYLLLMAGKKDAVEHIRRHE
                     KAILSDKQRG"
     gene            1414..2319
                     /gene="pfkB"
                     /locus_tag="ACZPCL_04580"
     CDS             1414..2319
                     /gene="pfkB"
                     /locus_tag="ACZPCL_04580"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HIR1161791.1"
                     /translation="MIYTVTFNPALDYVISVDHFTPGKINRTVSENMICGGKGINVSA
                     LLANLGYESTALGFVAGFTGAEIEKRAKELGFASDFIHVKKGMSRINVKMKSDEETEI
                     NGMGPDIQQEDVDALFEKLDGLKEGDVLVLSGSIPSMISHHIYEEIMKRLEDRGVRMV
                     VDAEKDLLLDVLPLKPFLIKPNNHELGQMFGRKLKTEDEIIECAKELQDRGAVNVLVS
                     MAGDGALLVAEDGHIYKEGVCRGTVKNSVGAGDSMVAGFLAGYLEKGDYEYALKLGTA
                     SGGATAFSYGIGTKELIMELLGQLQ"
     gene            2383..4368
                     /locus_tag="ACZPCL_04585"
     CDS             2383..4368
                     /locus_tag="ACZPCL_04585"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HIR1161792.1"
                     /translation="MRITELLKKESIELGVKVADKNAAIDKLISLMDAGGRLNSTQGY
                     KEGILAREALGSTAIGEGIAIPHAKVEAVKEPGLAAMVVPEGVDYEAFDGSLANLIFM
                     IAAPAAGDDVHLQALSRLSTLLMNPGFKESLIAAKDTDEFLDIIDKAENERFGEEERK
                     TEAAAENVTEDKAVEQKVDDGAEKSASAVNAHYRVLAVTACPTGIAHTYMAAENLENT
                     GKKLGISLKAETDGSGGAQNVLTKEEIAAAEAIIVAADKNVEMARFDGKPVIMVPVAD
                     GIHKAEVLINQAVSGTVPIYHYSGDASGAASEGENDSVGRQIYKHLMNGVSHMLPFVI
                     GGGILIALAFLLDDYSIDPSNFGKNTPLAAYLKTIGEQAFGMMLPILAGFIAMSIADR
                     PGLAVGLVAGLIAKMGSTFANPAGGDVNAGFLGALFAGFAGGYIVLGLKKLFSKLPKS
                     LEGIKPVLLYPVIGIFLAAVVTTFINPYMGMINDGLTHFLNGMGGTSRVVLGMILGGM
                     MSIDMGGPFNKAAYVFGTAQLAEGNFEVMAAVMAGGMVPPIAIALCTTFFKKKFTEKE
                     RQSGVVNYVMGLSFITEGAIPFAAQDPLRVIPSCLIGSAIAGGLSMAFGCTLRAPHGG
                     LFVLPTIGNPVMYLAAVVIGAVVGCLILGILKKNVEE"
     gene            4609..4681
                     /locus_tag="ACZPCL_04590"
     tRNA            4609..4681
                     /locus_tag="ACZPCL_04590"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4642..4644,aa:Val,seq:tac)
     gene            4699..4772
                     /locus_tag="ACZPCL_04595"
     tRNA            4699..4772
                     /locus_tag="ACZPCL_04595"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4733..4735,aa:Met,seq:cat)
     gene            4779..4851
                     /locus_tag="ACZPCL_04600"
     tRNA            4779..4851
                     /locus_tag="ACZPCL_04600"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4812..4814,aa:Val,seq:tac)
     gene            4868..4941
                     /locus_tag="ACZPCL_04605"
     tRNA            4868..4941
                     /locus_tag="ACZPCL_04605"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4902..4904,aa:Met,seq:cat)
     gene            5145..6071
                     /locus_tag="ACZPCL_04610"
     CDS             5145..6071
                     /locus_tag="ACZPCL_04610"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR1161793.1"
                     /translation="MYHFIINPNAGSGRGWKVWRAIAGYMDRHNIEYEAVLTNGIGEA
                     RDAARELTKEAGKPCFLIVVGGDGTMNEVLDGASFHGPLNLGYIPAGTGNDLWRSLHM
                     PASPVKCLKKQLQPRHFSMIDYGVLSYGKGEPFHRRFLVSAGIGFDAAVCQAALDSRL
                     RSRLGHMGFRRLSYLLLGIGQFFKCRSSRGYIVLDGVKRVEFNNILFISCHIQPSEGG
                     GFVFAPKADGKDGLMNICVVSHSNRMKLIPLLLGAMTGRKKRYKGTRTYECREVKIHT
                     DAPLPVHVDGEVCGNVNDIQAECIPRKVRMMF"
     gene            6129..6677
                     /gene="ispF"
                     /locus_tag="ACZPCL_04615"
     CDS             6129..6677
                     /gene="ispF"
                     /locus_tag="ACZPCL_04615"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR1161794.1"
                     /translation="MRIGQGYDVHKLVEGRKLILGGVEIPYEKGLLGHSDADVLVHAV
                     MDALLGAAALGDIGQHFPDTDPRYEGISSIELLKKVGELLEEKGYVVENIDATIIAQR
                     PKLLNYRPKMAENIAQALHLDVSRVSVKATTEEGLGFTGTGEGISSQAITLLTEVADY
                     CYDNKIVNDSCTGCGGCCGSQK"
     gene            6732..8141
                     /gene="cysS"
                     /locus_tag="ACZPCL_04620"
     CDS             6732..8141
                     /gene="cysS"
                     /locus_tag="ACZPCL_04620"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR1161795.1"
                     /translation="MKIFNTLTRRKEEFVPLTPGQVKMYVCGPTVYNLIHIGNARPMI
                     VFDTVRRYFEYKGYDVQYVSNFTDVDDKIIKKANEEGVDAEVISKRYIEECKKDMAAL
                     NVKPATVNPQATQEIQGMLTMIQTLIDKGHAYVADDGTVYFKVKSFKEYGKLSHKNLD
                     ELQSGFRELKVSGEEVKEDPNDFVLWKPKKEGEPYWESPWCQGRPGWHIECSVMAKHY
                     LGDQIDIHAGGEDLIFPHHENEIAQSECANDKTFANYWMHNGFLNIDNKKMSKSLGNF
                     FTVRDIAEKYDLQVLRFFMLSAHYRSPINFSAELMEASKNGLERILTAVDRLKELDAK
                     AEGAAETCAEQEKMVEVQKLREKFEAAMEDDFNTADAVSAIFELVKLANSTADASSTK
                     SYVSCLLKEIEQLCDVLGIITERKTEVLDSEIEEMIAARQQARKDRNFALADEIRGKL
                     LDMGIVLEDTREGVKWKRA"
     gene            8126..8611
                     /locus_tag="ACZPCL_04625"
     CDS             8126..8611
                     /locus_tag="ACZPCL_04625"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HIR1161796.1"
                     /translation="MEESITLKDFLGFYKKTMEMGPVDAGTYSPLVLAYIGDAVYEVM
                     IRAKVINKGSIQVNKLHKRSAELVKAGTQAAMIRLLEEDLTEEEHAVYKRGRNAKSAT
                     MAKHATMVDYRTATGFEALVGWLFLEERFDRLTELVSLGLTKIGAMETKTTPAPEQKG
                     E"
     gene            8615..9361
                     /gene="rlmB"
                     /locus_tag="ACZPCL_04630"
     CDS             8615..9361
                     /gene="rlmB"
                     /locus_tag="ACZPCL_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR1161797.1"
                     /translation="MRYEELTIEGRNAVLEAFRSGKTIDKLFVLDGCQDGPVRTIVRE
                     AKKYDTIINYVAKERLDQMSETGKHQGVIAYAAAYEYAEVEDMLKLAEEKGEPPFLFL
                     LDGIEDPHNLGAIIRTANLAGAHGVIIPKRRAVGLTATVAKTSAGALNYTPVAKVTNL
                     TATMEDLKKRGLWFVCADMGGDVMYKMNLTGPIGLVIGNEGEGVSKLVKEHCDMVASI
                     PMKGDIDSLNASVAAGVLAYEIVRQRLAKQ"
     gene            9441..10295
                     /locus_tag="ACZPCL_04635"
     CDS             9441..10295
                     /locus_tag="ACZPCL_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR1161798.1"
                     /translation="MMTAGQTGLVTGLAAEAHVLTPIASGATVYKNDKATLDASNTAS
                     GYIMVKYTGSVGKIKVQVSKSGSETYTYDLNSSGTYEVFPLSEGNGTYQVKVFENIQG
                     TQYSQAFSQSLNVDITDTFGPFLYPNQYVNFNPASAAVQKGAELSAGATDQIGVVTAI
                     YNYVINNLTYDTAKAQSVQSGYLPNVDVVLAQHTGICFDYAALMTAMLRSQNIPTKLV
                     VGYTGSLYHAWINVYLDGQGWVDNVIYFDGHDWKLMDPTFASSGKQSQEIMRYIGNGS
                     NYKAKYSY"
     gene            10341..11414
                     /locus_tag="ACZPCL_04640"
     CDS             10341..11414
                     /locus_tag="ACZPCL_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR1161799.1"
                     /translation="MADLEKKEEKSRQWKPYSCSELSAFCLQVSLLLEAAVPLDEGFS
                     IMAEDAADEKERQMLLYMSEGAELGDPCFKIFKDTGVFPDYVIRMAKLGQETGTLDQM
                     MKSLSDYYEKEDRLIKTLKNAVRYPAMMILMLLVVLFVLFVKVMPIFSKVYEQLGAEM
                     SPVAQSAIRLGGTFSGAALVLSAVIAVVMAGVAAASKMGIRIGIADKITTEIKNRSKI
                     AHAIAKRRFTSVLSLTLRSGLEFEKGLELAGELAENDRIAEQVKKCEEKLELGESYYE
                     ALKETGMFSGFYIQMIKVGTRSGHLDSVMEEISNDYEEIADTAIDNMIARFEPTIVAV
                     LAVSVGLVLLSVMLPLAGVLSAI"
     gene            11467..11799
                     /locus_tag="ACZPCL_04645"
     CDS             11467..11799
                     /locus_tag="ACZPCL_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161800.1"
                     /translation="MRVRKDKEDKGEFRGLLLSFLLFLAAAAAFGAGSLGFLNRTGQR
                     SQETLRKAITRACIQCYAIEGRYPPSVEYLEENYGIQIDHDKYNVFYDGFASNIMPEI
                     TVIPVEQE"
     gene            11819..12313
                     /locus_tag="ACZPCL_04650"
     CDS             11819..12313
                     /locus_tag="ACZPCL_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4860 domain-containing protein"
                     /protein_id="HIR1161801.1"
                     /translation="MKERTQTGGQAISGLFTRLLFLVFVLCALFSVLMGSRVYENITK
                     RSDENFSGTTALSYIANKIRQGDREDAVDIIEVDGTPVLQLKQKVGDEMYHTWIYWKD
                     GQIRELFTDENSGLGLEDGLEILECDGLSMEKDGNSVKLQTLGSGGGRLELSLRSGGN
                     GADE"
     gene            12306..12839
                     /locus_tag="ACZPCL_04655"
     CDS             12306..12839
                     /locus_tag="ACZPCL_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161802.1"
                     /translation="MSRNRGSGSGPFLMEMVIAAGFFMLCVAVCVSAFVMADHLSRVG
                     QDVNHGVLAAESLVERVKVGNTGDFGTYEPDEVFGTVFENWKTKDPEAKAVYQYIKDG
                     GEVRNYQILWDKEWNSYPDTKELEKNGKKLAYVGLITTGVKDQMKEIAVVIFGYGTYG
                     DQGYKIYHLFAEQYVKP"
     gene            12901..13377
                     /locus_tag="ACZPCL_04660"
     CDS             12901..13377
                     /locus_tag="ACZPCL_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161803.1"
                     /translation="MAGQMSKHKINIGLTSLILIFIILCLATFSLLSLSSARGDQSLA
                     ARSARAVTEYYRADAEGEKWLKQADAILQKEMTKKAMDQEEIQALAKKMALELGCDGD
                     EETGFVSTDISMDRGQALHIDLALTGDENRYEVRSWYVYDSGNYEIDDFMPVWDGK"
     gene            13432..14496
                     /locus_tag="ACZPCL_04665"
     CDS             13432..14496
                     /locus_tag="ACZPCL_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR1161804.1"
                     /translation="MEVMELLTQAVEQNAADIFLVPGMPFSYKIGGRIICQGEEKIMP
                     DQMNQMITRIYEIAKNRSMERVLSHGDDDFSFAIPGVSRFRANVFRQRGSLAAVIRVV
                     RFELPDPGQLKLPDVIMGISKLTRGMVLVTGPAGSGKSTTLACIIDEINKTRNAHVIT
                     LEDPIEYLHRHKKSVVTQREVATDTDSYVSGLRASLRQAPDVILLGEMRDHETISIAM
                     TAAETGHLILSTLHTLGAANTIDRVIDAFPPNQQQQIRTQLSMVLDAVICQQLVPTLD
                     GGERPVFEIMFLNNAIRNMIRESKTHQIDGIIATSQEEGMISMDNSLLALYRQNVISK
                     DTAIIYSSNTELMEKKIARL"
     gene            14537..15481
                     /locus_tag="ACZPCL_04670"
     CDS             14537..15481
                     /locus_tag="ACZPCL_04670"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIR1161805.1"
                     /translation="MKTDKRKIVIVGTGMVGMSYAYCLLNQSVCDELVLIDVDKKRAE
                     GEAMDLNHGLAFAGSSMKIYAGDYESCQDADLVLIAAGVAQKQGETRLDLLKRNTQVF
                     RSIIEPVTASGFNGIFLVATNPVDIMTRITCALSGFNPRRVLGSGTALDTARLRYLLG
                     EYLGVDPRNIHAYVMGEHGDSEFVPWSQAFLATKSISQLDWELESSLTAHLLRIEEEV
                     RTAAYKIIEAKKATYYGIGMALTRITKAILGDEHSVLTVSAMLRGEYDQMDVFAGVPC
                     IINQNGVQRILPLSLTDEEKEKFNGSCDILREGYEGIL"
     gene            15547..16113
                     /locus_tag="ACZPCL_04675"
     CDS             15547..16113
                     /locus_tag="ACZPCL_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1161806.1"
                     /translation="MKVLLLNGSPRKNGCTYTALNVVADALKAEGIDAEVFQAGDPDM
                     ENVKAAAAKLKDSDALVVGSPVYWASPSGQIIEFMDRLASVAGKDMLYKPAAAVASAR
                     RAGTTATLDVLTKYFSYHQMPVVSSCYWNMVHGNTPEEVMKDEEGVQIMRVLGKNMAW
                     LLKCIEAGKKNGVAVPTAEDKIKTNFIR"
     gene            complement(16254..16326)
                     /locus_tag="ACZPCL_04680"
     tRNA            complement(16254..16326)
                     /locus_tag="ACZPCL_04680"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16291..16293),aa:Thr,seq:ggt)
     gene            complement(16363..16435)
                     /locus_tag="ACZPCL_04685"
     tRNA            complement(16363..16435)
                     /locus_tag="ACZPCL_04685"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16400..16402),aa:Thr,seq:ggt)
     gene            complement(16525..>18115)
                     /locus_tag="ACZPCL_04690"
     CDS             complement(16525..>18115)
                     /locus_tag="ACZPCL_04690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding response regulator"
                     /protein_id="HIR1161807.1"
                     /translation="AQAVYSVDLTGDRLEKIFYHTTECEFDLNIKFPCSYDEYCLNRS
                     RFVTEDTQENYRIVDSSAKLLERFRSGTKQVTVEYREQNENGEIFWLQKTVLMSQDTV
                     YNSETGKESTVIHGMILFKNTSVFHEKEQQERERLQVAFEEADSASKAKTEFLNRMSH
                     DIRTPINGIMGMLEIIRKNREDHARVDDSLEKIYVSSSHLLALVNDVLDMSKLESRQA
                     VFKPVDFDLSQLMRDVSYLVKAQIMEMGLTHRTHRQNIQHVLLSGYPLQLRQIMLNLF
                     SNAIKYNKKGGFIDTGAKEISFDGTTVVYEFFISDTGIGMSEAFLKNQLFEPFSQEKS
                     DARTQYKGTGLGMSIVKGLIRQMNGSIEVESSPGEGTTFTFRLPFRLAKEEANDAKAD
                     TASETGKELEGIHVLLVEDNEINMEIAEFYLTDRGATLDKAWNGKEALEQFERSPEGT
                     YDIILMDVMMPVMNGLEASRQIRSLSRPDGNMIPILAMTAQDAISVSSQCRQYGMDDY
                     VLKPADPEQLTKMILKYVKTI"
BASE COUNT         5323 a         3506 c         4695 g         4591 t
ORIGIN      
        1 gacgcatgca tgatgtgata atcacgggtg actgtccgct ccaggtacat gaaccagggc
       61 aggtagatga atgcgtagga cagcagccat gcatgcctgt atttgtgtaa aaattcgcgg
      121 atctttttca tagtttcctt tcttaatcct atatagtatc aaacaaattt tgaaatagtg
      181 tttacattgt ttctttttta tcgtggagat tatagcacgg taaattttga aaaacaaggg
      241 gagtcaggaa atattaataa agttctcttc tatttttatt tttgttatga tatatttatt
      301 attaagaaat aaatttacat gagattggtg gcgtgagaaa tgagtgaagc acgaagcgtt
      361 ctgggagtga tctgttctgc ctatgacagt ttttttgaag cagagaaaaa gatcgcggac
      421 tatattatgg aacataaaga gcaggctgtg gatatgacag tgggagaact ggcaaaggcc
      481 agcggtacca gtgatgctac tgtgtcaaga ttttgccgca gatgtggttt taagggattt
      541 cagaacctga aactgacact tgcaagagag atcctggagg aagagcatca gagccaggaa
      601 gtgaccaatg atattgacag gacggatctg gaacagtctt taaagaacat tcttgcgaat
      661 aaaattgcgg aactgacaga gactataaag atgatggaca ttaaaaatct ggaggttatt
      721 ctggataagc tggagcatgc aaggatggtg cagctggcag ctgtgggaaa tacgatccct
      781 gtggcattag acggagcttt taagctgaac cagctgggta ttccggcagt ggcaggggat
      841 atctgggaag cccaggctgc gtatgctttt aatctggggc cggaggatgt ggttttgatc
      901 atttcaaact ctggttcttc caagcggttg gagacgctgg ctttgggagc gaaggaaaat
      961 ggaagtaccc tggtgcttat tacgaataac agggagtcac cgttagcaca gatctgtgat
     1021 tataagatca ttactgctac cagggaaaaa cttctcacag aggaattctg gttttccaga
     1081 gtcacagcca ctactgtgat cgagatcatt taccttcttt tgatggcggg aaagaaggat
     1141 gcggttgagc atatcagacg ccatgaaaaa gctatattat ctgataaaca gaggggatag
     1201 atacacaaga taatatatca ggcagcacct aacaatctgc acaaaagaga gtcgcatttt
     1261 acagaaaaat gcggctcttt ttatctaaag tgctgaaaat tagtgtgaaa ttaattttct
     1321 caaagaacac tattgacgaa aaagaaaaat catattatag taaagccata aggataagaa
     1381 ataaaatttc ttaaaagtaa ggagaagtga cgtatgattt acacggtgac ttttaatccg
     1441 gcactggact atgtgatctc tgtggatcat tttactccag gtaagatcaa ccggaccgta
     1501 tcagaaaaca tgatctgcgg gggaaaaggg atcaatgtat cggctctttt agcaaatctg
     1561 ggttatgaga gtacagcact gggctttgta gcaggtttta caggagctga gatcgaaaag
     1621 agagcgaaag agctggggtt tgcatcagat ttcattcatg taaagaaggg catgtcccgt
     1681 atcaatgtga agatgaaatc agacgaagag acggagatca acggcatggg accggatatc
     1741 cagcaggaag atgtggatgc tttgtttgag aagctggatg gcctgaaaga aggggatgtg
     1801 ctggtacttt ccggaagtat tccttctatg atcagtcatc atatttatga ggagatcatg
     1861 aagcggctgg aggacagagg tgtacgtatg gttgtggatg cggaaaagga tctgctgttg
     1921 gatgtactgc ctttgaagcc atttcttatt aagccaaata atcatgaact gggtcagatg
     1981 tttggaagaa aattgaagac ggaagatgag atcatagagt gcgcaaagga actgcaggac
     2041 cggggtgctg taaatgtcct ggtttctatg gcaggagacg gcgcattgct ggttgcagaa
     2101 gatggacata tttataagga aggtgtctgc aggggaacag tgaaaaactc cgtaggtgcc
     2161 ggtgactcta tggtagcggg atttttagcc ggttatctgg aaaaaggaga ttatgagtac
     2221 gctttgaagc tgggaacagc cagtggcggc gcaaccgcat tttcctatgg tattggtaca
     2281 aaggaactga tcatggagtt gttaggacag ttacagtaag cttacgatga aataaaatat
     2341 gggcatgaaa aagcagattg aaatatagaa ggaggtatac acatgcgtat tacagagctg
     2401 ttgaaaaagg aaagcattga actgggcgtg aaagtggcag acaagaacgc agccattgac
     2461 aaactgatct cactgatgga tgctggtgga cggcttaaca gtacacaggg atataaggaa
     2521 ggaattttag caagagaggc acttggaagt acagccattg gtgaaggaat tgccatccca
     2581 cacgcaaagg tagaggcagt aaaagaaccg ggacttgcag caatggtagt tccggaaggc
     2641 gtggattatg aggcatttga cggctccctg gcaaatctga tctttatgat cgcagcaccg
     2701 gcagcaggag atgatgtgca tttacaggca ttatccagac tgtctacctt attgatgaac
     2761 ccgggattta aggagagcct gatcgctgca aaggatacag atgagttttt agatatcatc
     2821 gacaaggcgg aaaatgagcg ctttggcgaa gaagagagga aaacagaggc agctgcagaa
     2881 aatgtgacag aggataaggc tgtggaacag aaggtagatg atggggcaga aaagagtgct
     2941 tcggcggtta atgcacatta ccgggtgtta gctgtaactg cctgccctac aggtattgcc
     3001 catacgtaca tggcagcgga aaatctggaa aatacaggaa agaagctggg gatttccttg
     3061 aaggctgaga cagatggctc cggcggtgcc cagaatgtgc tgacaaaaga ggagattgcg
     3121 gcagcagagg cgatcatcgt agcagcggat aagaacgtgg aaatggcccg ttttgacgga
     3181 aagccggtga tcatggttcc tgtagcagat ggtatccaca aggcagaggt tctgatcaac
     3241 caggcagtaa gcggaaccgt accgatctat cattactcag gagacgcttc cggcgcagct
     3301 tctgaagggg agaatgacag cgtaggaaga cagatctaca agcatctgat gaacggtgta
     3361 tcccacatgc ttccatttgt gatcggcggc ggtatcctga tcgcactggc attcttactg
     3421 gatgattatt ctattgatcc atccaatttt ggtaagaaca cacctcttgc agcttactta
     3481 aagaccattg gtgagcaggc atttggcatg atgcttccga tccttgcagg ctttattgcc
     3541 atgagtattg cagaccgtcc tggtcttgca gttggtctgg tagcaggtct gatcgctaaa
     3601 atgggttcta cttttgcaaa tccggctggc ggagatgtaa acgcaggttt ccttggcgca
     3661 ttatttgcag gttttgcagg tggttacatt gtattaggac tgaagaaact gttttccaaa
     3721 cttccaaagt cactggaagg tatcaaacct gtgttattgt atccagtgat cggtattttc
     3781 cttgcagcag tcgttactac ctttatcaat ccatatatgg gcatgatcaa tgatggactg
     3841 acccacttct taaatggaat gggcggaacc agccgtgtcg ttttaggtat gatccttggc
     3901 ggcatgatgt ctattgatat gggtggtcct ttcaacaaag cagcttatgt attcggaaca
     3961 gcgcagttag cagaaggaaa ttttgaagta atggcagcgg taatggcagg tggtatggtg
     4021 cctccgatcg cgatcgctct ttgcactact ttctttaaga agaaatttac ggaaaaagag
     4081 cgtcagtccg gtgttgtaaa ctacgtaatg ggtctttcct tcattacaga aggcgctatt
     4141 ccatttgcag cgcaggatcc tcttcgtgta atcccgtcct gtctcatcgg ttctgccatt
     4201 gcaggtggtc tgtccatggc atttggctgt acgttaagag cacctcacgg cggattattc
     4261 gtactgccta ccatcggcaa tccggtgatg tatctggcag cagttgtgat cggagctgtt
     4321 gtaggttgct taatcctggg aattctgaaa aagaacgtgg aagaataaaa tattgcatct
     4381 tcccctacat cgcaggggaa agcagatata tacttaaggg agtattttac agcagcccgg
     4441 ccaaggggag aaacggccgg gctgtttttg cgccaggaca cattctttgt atatgaaaaa
     4501 gccatgaaaa taaatggaaa aataattttc aaaaagtgtt gacaattcct acaatttgaa
     4561 ttatactatt caagcaatcg catttgagac aaatgtgaaa gcggatacgg ggtcttagct
     4621 cagctgggag agcatctgcc ttacaagcag agggtcacag gttcgagccc tgtaggcccc
     4681 atccaattaa ataattatgg cggaatagct cagatggcta gagcacacgg ttcatacccg
     4741 tggtgtcgag agttcgaatc tcccttccgc tactgtatgg ggtcttagct cagctgggag
     4801 agcatctgcc ttacaagcag agggtcacag gttcgagccc tgtaggcccc atccaataac
     4861 taaatatggc gagatagctc agttggctag agcacgcggt tcatacccgc ggtgtcgagg
     4921 gttcgaatcc ccctctcgct atgaaaaaag caatttgcgt agtgcagatt gcttttttgc
     4981 tatatacaaa tatacattgg caggccactg gacgagcagt tcatggacgg tgacataagg
     5041 ggcattttta atagaaattt tcgatgcgtt ttatattctt ttcggtacca ttatgctata
     5101 ataaaatgag cacagaaaaa caagaaagga caccctgttt gcctatgtac cattttatca
     5161 ttaacccaaa cgcaggctcc ggcagaggat ggaaagtatg gagagctatt gccggttata
     5221 tggaccggca caatattgaa tatgaagcgg ttctgacaaa tgggatcggc gaagccagag
     5281 acgctgcaag agagctgaca aaggaagcag gaaagccatg cttccttatt gttgtaggcg
     5341 gagatggcac gatgaacgag gtattagatg gtgcatcctt tcatggtcct ttaaatcttg
     5401 gctatattcc ggctggaaca ggaaatgatc tgtggcgaag tctgcacatg ccggcaagcc
     5461 cagtgaagtg cctgaaaaag cagcttcagc caagacattt ttccatgatc gattatggtg
     5521 tcctttccta tggaaaagga gaaccttttc acagacgttt cttagtcagt gccgggatcg
     5581 gctttgacgc tgcagtctgt caggcagccc ttgactcccg gcttcgcagc cgtttaggac
     5641 atatgggttt tcgcaggctt tcctatttgc tccttgggat cggccagttt tttaaatgca
     5701 ggtcttccag aggatatatt gttttagatg gtgtaaaacg ggtagaattt aataacatcc
     5761 tgtttatatc ctgccacatc cagccatcag aaggcggcgg tttcgtcttt gccccaaaag
     5821 cagacggaaa agacgggctg atgaacatat gcgtagtcag tcattctaac cggatgaaac
     5881 tgatccctct tcttttggga gccatgaccg gcaggaaaaa gcgttataaa ggaaccagaa
     5941 cctatgagtg cagggaagtg aagatacaca cagatgcccc gcttccagtt catgtggacg
     6001 gagaagtgtg tggaaatgta aacgatatac aggcagaatg tattccgcgg aaagtacgga
     6061 tgatgttttg atcacagggt ctgccagaca gaattttata aatcagttat aaaggagaca
     6121 taaaataaat gaggattgga cagggatatg acgtacacaa actggtagaa ggaagaaaac
     6181 tgatccttgg cggtgtggag attccttacg aaaagggact gttaggccat tctgatgcag
     6241 atgtgcttgt acacgctgta atggatgctc ttttaggcgc agctgcttta ggggatatcg
     6301 gacagcattt tcctgatacg gatccaagat atgaggggat atccagtata gaactgctga
     6361 aaaaagtagg ggaattactg gaagaaaaag gctatgtagt ggaaaatatc gatgccacca
     6421 tcattgccca gcgtcccaaa cttttaaact accgtccaaa gatggcagaa aacattgccc
     6481 aggcactgca tctggatgta tccagagtca gtgtaaaagc cactacagaa gaggggcttg
     6541 gctttacagg aacaggggaa ggaatttcct cccaggcaat tacattattg acggaagtag
     6601 ctgattactg ctatgataat aagatagtaa atgatagctg cacaggctgt ggaggctgct
     6661 gcggctctca aaaataaaac tggggataaa tatgtaagtt tgtaaagctt acagaaatga
     6721 ggagaatgac tatgaagatc tttaatactc tcacaagaag aaaagaagaa tttgtcccat
     6781 taacaccggg acaggtaaaa atgtatgtat gcggacctac tgtttacaat ctgatccaca
     6841 ttggaaatgc aagacctatg atcgtatttg atacagtacg ccgttatttt gagtacaagg
     6901 gctatgatgt acagtatgta tccaacttca ctgacgtaga tgacaagatc atcaagaagg
     6961 caaatgaaga aggcgtagat gcagaagtga tctccaagcg ctatattgaa gagtgcaaaa
     7021 aggatatggc agcgttaaat gtaaagcctg caactgtaaa tccacaggca acccaggaga
     7081 tccagggcat gcttaccatg atccagaccc tgatcgataa gggacatgct tatgtagcag
     7141 atgacggaac tgtatacttc aaggttaaat catttaaaga atacggaaaa ctgtcccata
     7201 agaacttgga cgaacttcag tccggtttcc gtgaattaaa ggtaagcggt gaagaagtaa
     7261 aggaagatcc aaacgacttc gtattatgga agccgaaaaa agaaggagag ccttactggg
     7321 agtctccgtg gtgccagggc cgtccgggct ggcatattga gtgctctgtt atggcaaagc
     7381 actatcttgg cgaccagatc gatatccacg caggcggcga ggacctgatc ttcccacacc
     7441 atgaaaatga gatcgcgcag agtgagtgtg caaatgataa gacatttgca aattactgga
     7501 tgcacaatgg tttcttaaat attgacaata agaagatgtc caaatccctt ggaaacttct
     7561 ttactgtacg tgatattgct gagaaatatg atctgcaggt actgcgtttc ttcatgttaa
     7621 gcgcacacta cagaagcccg atcaacttca gcgcagagtt aatggaagct tccaagaacg
     7681 gcttagagcg tatccttaca gcagtagacc gcttaaaaga actggatgca aaggcagaag
     7741 gtgcggcaga gacctgtgca gagcaggaaa agatggtgga agtacagaag ctgcgtgaaa
     7801 aattcgaggc agctatggaa gatgacttta acacagcaga tgctgtttct gctatcttcg
     7861 agctggttaa actggctaac tccacagcag atgcttccag caccaagtcc tatgtttcct
     7921 gcctgttaaa ggagatcgaa cagttatgtg atgttttagg cattatcaca gagagaaaga
     7981 cagaagttct tgacagcgag atcgaggaaa tgatcgcagc aagacagcag gcaagaaaag
     8041 acagaaactt tgctcttgca gatgagatca gaggaaaact tctggatatg ggtattgttc
     8101 tggaagatac cagagagggc gtaaagtgga agagagcata actttaaagg actttctggg
     8161 attttataaa aagacaatgg aaatgggccc tgtagacgca gggacctatt ctccgctggt
     8221 gctggcatat attggggatg cagtctatga agtgatgatc cgcgcaaaag tgatcaataa
     8281 aggcagcatc caggtaaata aacttcataa aagaagcgca gaacttgtaa aggcaggtac
     8341 ccaggcagcg atgatccgtc ttttagaaga agacctgaca gaagaggaac atgctgttta
     8401 taagagaggc cgcaatgcca aatcagctac tatggcaaaa catgccacga tggtagatta
     8461 ccgtacagca accggttttg aggcactggt aggctggctg ttccttgagg agcgctttga
     8521 ccgccttaca gagcttgtaa gcctgggcct tacaaagatc ggcgcaatgg aaacaaaaac
     8581 aacaccggca cctgaacaaa aaggagaata atagatgaga tacgaagaac tgactattga
     8641 aggccgcaat gccgtattag aggcattccg ctccggaaaa accattgaca agctgtttgt
     8701 attagacggc tgtcaggatg gtccggtgcg taccattgtc agagaagcta aaaaatacga
     8761 taccattatc aattatgttg caaaagagcg cttggaccag atgtctgaga caggaaaaca
     8821 ccagggtgtc atcgcctatg cggctgctta tgaatacgca gaggtagaag atatgttaaa
     8881 actggcagaa gaaaagggcg aaccgccatt tttattcctt cttgacggga tcgaggatcc
     8941 tcacaatctg ggagctatca tccgtactgc caatctggct ggtgcacatg gggtcattat
     9001 cccaaagcgc cgtgcagtgg gtctgacagc taccgttgcg aagacatcag ccggtgcgct
     9061 taattatact ccggttgcaa aagttacaaa cctgaccgct accatggaag acttaaagaa
     9121 gagaggtctc tggtttgtat gtgctgatat gggtggtgac gtgatgtata aaatgaacct
     9181 taccggccct attggacttg tgatcggaaa tgaaggggaa ggcgtcagca aacttgtaaa
     9241 agaacactgc gatatggtag catccattcc tatgaagggt gatattgact ccctgaatgc
     9301 ttccgttgca gcaggtgtat tagcttatga gatcgtgcgt cagcgtctgg caaaacagta
     9361 gatgcgcgaa agtacaagga gttgaacagg cagtgaaaaa aacaaagaag atcctatccg
     9421 ttttactggc ggcagcaatt atgatgacag caggtcagac cggtctggta acaggactgg
     9481 cagcagaggc ccatgtactt acacccattg cctccggtgc tactgtatac aaaaatgata
     9541 aagcaaccct ggatgcgtcc aatacagcgt ccggttatat catggtaaaa tatacaggca
     9601 gtgtgggaaa gatcaaagta caggtgtcaa aaagcggttc agaaacctat acctatgatc
     9661 tgaacagcag cggtacctat gaggtatttc cgttatcaga gggaaatgga acctatcagg
     9721 taaaagtgtt tgaaaatatc cagggaaccc agtattccca ggcattcagc cagagcctga
     9781 atgtggatat cacagatact ttcggaccgt ttctgtatcc aaaccagtat gtgaacttta
     9841 atccggccag tgcagcggtc cagaaaggcg cagaactttc agcaggggcc acagaccaga
     9901 tcggagtggt tactgctatt tacaactatg tgatcaataa cctgacttat gacacagcaa
     9961 aagcacagtc tgtacagtct ggttatcttc caaatgtaga tgtggttctt gcacagcata
    10021 cggggatctg ctttgattat gctgccttaa tgacagccat gctgcgttcc cagaatatac
    10081 caaccaagct ggttgtggga tatacaggaa gcctttatca tgcatggatc aatgtatatc
    10141 tggatggcca aggatgggta gacaatgtga tctattttga cggacatgac tggaaactga
    10201 tggatcccac ctttgcttct tccggaaagc agagccagga gatcatgcgg tatattggaa
    10261 acggttctaa ttataaggct aaatacagct attagactga tcgcattaca gtagcgtcac
    10321 agaggaaagt aggaaagaca atggcagatc ttgaaaagaa ggaagaaaaa agcagacagt
    10381 ggaagccata ttcctgcagt gaactgtccg ctttctgcct gcaggtctct ctgcttctgg
    10441 aagcagcagt gcctttagat gaaggatttt ctattatggc agaggatgct gcagatgaaa
    10501 aagagcggca gatgcttttg tatatgtcag agggagcgga actgggagat ccctgcttta
    10561 agatctttaa agatacagga gtttttcctg attacgtgat ccgcatggca aaactgggac
    10621 aggaaacagg taccctggat cagatgatga agtcgttgtc tgattactat gaaaaagagg
    10681 accgccttat caagacactg aaaaatgcag taaggtatcc ggctatgatg atccttatgc
    10741 tgttagtggt actgtttgtg ctgtttgtaa aggtaatgcc tattttttca aaagtgtatg
    10801 aacagttagg ggcggagatg tcaccggtag cccagtcagc tatccggctg gggggtacat
    10861 tcagtggtgc tgcactggtc ctgtcagccg ttatagctgt agttatggca ggggttgctg
    10921 ctgcttctaa aatggggatc cgcatcggga tcgctgataa gatcaccaca gagatcaaaa
    10981 accgcagtaa gattgcccat gcgattgcaa aaaggcgttt tacatctgtc ttatccctta
    11041 ctttaagaag cggacttgaa tttgaaaagg gtctggaact ggcaggagag ctggcagaaa
    11101 atgatcgcat tgcagaacag gtaaagaaat gcgaagaaaa gctggagctg ggagaaagct
    11161 attatgaggc tttaaaggaa acaggtatgt tttccggttt ctatatccag atgatcaaag
    11221 tgggaaccag aagcggtcat cttgacagtg ttatggaaga aatttcaaat gattacgaag
    11281 agatcgcaga cacagccatt gacaatatga tcgcaagatt tgaaccgacc atcgtggcag
    11341 tgctggcagt ttctgtaggt ctggtactgc tttccgttat gcttcctctt gcaggtgtgc
    11401 tttcagcaat ctgacagaag gcatgaggga agacagaaag gttcagaccg gagggatcct
    11461 atgaaaataa gagtgagaaa agacaaagag gataaagggg agtttagagg actcctgtta
    11521 tcctttttgc tgtttttggc ggcggcagca gcgtttgggg ccggttccct tggcttttta
    11581 aacaggaccg gtcagagaag tcaggaaaca ctgagaaaag ccattacaag agcctgcata
    11641 cagtgttatg ccattgaggg caggtatccg cccagtgtgg agtatctgga ggaaaattac
    11701 gggatccaga tcgaccatga taaatacaat gttttttatg acggttttgc ttctaatatc
    11761 atgccggaga tcacagtaat cccggtagag caggagtgat taaggaggac gcaaacagat
    11821 gaaagaaaga acacagacag ggggacaggc cataagcggg ctttttacca ggcttctgtt
    11881 tctggtcttt gttttatgcg cccttttttc tgttcttatg ggaagccggg tatatgaaaa
    11941 catcacaaaa cgcagtgatg aaaatttttc aggtacaacg gctttaagtt atattgccaa
    12001 taagatacgc cagggtgaca gagaagatgc agtggatata atagaagttg atggaacgcc
    12061 tgttttacag ttgaagcaga aggtagggga cgagatgtac catacctgga tctactggaa
    12121 agatggacag atccgggaat tgtttacaga tgaaaacagt ggactggggc tggaggatgg
    12181 actggagatc ttggaatgtg acgggctttc catggaaaaa gatggaaact ctgtgaaact
    12241 gcagactctg ggatccggtg gaggaagact ggaactttca ttaagaagcg gaggaaatgg
    12301 ggcagatgag taggaataga ggatcaggtt ccgggccatt tttaatggaa atggtgatcg
    12361 ctgccggatt ttttatgctt tgtgtggctg tatgcgtgtc tgcttttgta atggcagatc
    12421 atttaagcag agtcgggcag gatgttaacc atggagtttt agctgctgaa agtctggtgg
    12481 aaagagtaaa agttggaaat acaggggatt ttggaaccta tgagccagat gaagtctttg
    12541 gaacagtctt tgaaaactgg aagacaaaag atccggaggc aaaggctgta tatcagtata
    12601 taaaagatgg cggagaggtc cggaattatc agatcctgtg ggataaagaa tggaacagtt
    12661 accctgatac aaaagaactg gaaaaaaatg ggaaaaaact ggcttacgta gggctgatca
    12721 ctacaggtgt taaggatcag atgaaagaaa tcgcagtagt tatctttgga tatggaactt
    12781 atggtgacca gggatataag atctaccatc tttttgcaga acagtatgtg aaaccatgat
    12841 cgcaggcgtt cctgaaaagg aaggatacta tagagaatac gatagaaagc aggtgggaga
    12901 atggccggac aaatgtcaaa acacaaaata aatataggtc tgacttccct gatccttata
    12961 ttcattatcc tgtgtctggc tactttcagt cttctttctt taagcagcgc cagaggagac
    13021 cagagtctgg cagccagaag tgcccgtgct gtgacagagt attaccgggc agacgcagaa
    13081 ggggaaaaat ggctaaagca ggcagatgcc attttgcaga aagagatgac gaaaaaagca
    13141 atggaccagg aagagatcca ggctcttgca aagaaaatgg cgttggaact tggctgtgat
    13201 ggagatgagg aaacaggctt tgtcagtacg gatatttcca tggaccgggg acaggcactt
    13261 catattgatc tggctcttac gggagatgaa aacagatacg aagtgagatc ctggtatgta
    13321 tatgacagcg gaaattatga gatcgatgat tttatgcctg tatgggatgg aaagtaagaa
    13381 ctgccggggt taaaagcagg aaaaacggga atgataacgg gaggaagagt aatggaggta
    13441 atggaacttc ttacacaggc tgtggaacag aatgcagcgg atatatttct ggttcctggt
    13501 atgccttttt catataagat cggtgggcgg atcatctgcc agggcgagga aaagatcatg
    13561 ccggaccaaa tgaaccagat gatcacaagg atctatgaaa ttgcaaagaa ccgcagtatg
    13621 gagcgggtgt taagtcatgg ggatgatgat ttttcctttg ccattcccgg agtgtccagg
    13681 ttcagggcga atgttttccg ccagagaggt tccctggcag cagtgatccg ggttgtgcgg
    13741 tttgaacttc ctgatccagg tcagttaaaa cttccggatg tgatcatggg gatttcaaaa
    13801 cttaccaggg gaatggttct tgtaacaggg cctgcaggca gcggtaaaag caccactctt
    13861 gcctgtatta ttgatgagat caataaaacc cgcaatgccc atgtgatcac gttggaagat
    13921 cctattgagt atcttcacag gcataaaaaa agcgtggtta cccagcgtga ggttgcaacg
    13981 gatacggaca gttatgtttc cggactgcgt gcttccttgc ggcaggcacc ggatgtgatc
    14041 cttcttgggg aaatgcgtga tcatgagacc atcagtattg ccatgacagc agcagaaaca
    14101 ggacatctga tcctttctac ccttcatacg ttaggggcgg ccaataccat tgaccgtgtg
    14161 atcgatgcct ttccgcccaa tcagcagcag cagatccgga cccagctttc catggtgtta
    14221 gatgcagtga tctgtcagca gctggtgcct accttagatg gcggggaacg tcctgttttt
    14281 gagatcatgt ttttaaataa tgccatccgt aacatgatcc gtgagtccaa gacccaccag
    14341 atcgacggta ttatcgctac ctcccaggaa gagggaatga tatctatgga taacagcctc
    14401 cttgctcttt acagacaaaa tgtgatcagc aaggataccg ctatcatcta cagcagcaat
    14461 acagagctga tggagaaaaa aatagcaaga ctgtaggaaa agtatccttt gctgactgat
    14521 tcagaaagga gctttcatga aaacagataa aagaaagatc gtgatcgtag gtaccggaat
    14581 ggtgggaatg agttatgcat actgcctttt gaatcagtcc gtatgtgatg aactggtact
    14641 gatcgatgta gataaaaagc gggcagaagg ggaagccatg gacttaaacc atgggcttgc
    14701 ttttgccggt tccagtatga agatctatgc cggggactat gaaagctgcc aggatgcaga
    14761 cctggtgttg atcgcagcag gagtggccca gaagcaggga gaaaccaggc tggatctgtt
    14821 aaagcggaac acacaggtat ttcgttcgat tatagagccg gtaactgctt ccggatttaa
    14881 cgggatcttt ctggtggcta ccaatccggt ggatattatg acacggatca cctgcgccct
    14941 ttccggcttt aatccaagaa gggtattggg aagcggtaca gcccttgata cggccaggct
    15001 ccgttatctc ttaggggaat atctgggagt ggatccaagg aatattcatg cttatgtaat
    15061 gggagaacat ggagacagtg aatttgttcc ctggagtcag gcttttctgg caacgaaatc
    15121 catttcccag ctggactggg agttagaatc ttctttaacg gcccatttac ttcgtattga
    15181 agaagaggtg cgtacagctg cctataagat catagaggca aagaaagcta cgtattacgg
    15241 gatcggtatg gctctgaccc gtatcacaaa agccattctg ggagatgaac acagcgttct
    15301 cactgtttcg gccatgctca ggggagaata tgatcagatg gatgtatttg ccggtgttcc
    15361 ctgcattatc aatcagaatg gggtacagcg gatcctgccg ttgtcactta cagatgaaga
    15421 aaaagagaaa tttaacggat cttgtgatat actaagagaa ggctatgagg gtatcttgta
    15481 gttaagcctg tttacccgcc tgtgcggact ggttcaggat gaatatatac atgaggagga
    15541 aaagaaatga aagttttatt attaaacgga agtccaagaa agaacggctg tacttataca
    15601 gcactgaatg tagtagctga tgctttaaag gcagaaggca ttgacgcaga agtgttccag
    15661 gctggtgatc cggatatgga aaatgtaaag gcagcagcag ccaagttaaa agactctgac
    15721 gcattagtgg taggctctcc tgtttactgg gcgtctccaa gcggccagat catcgaattt
    15781 atggaccgtc tggcatctgt agcaggaaaa gatatgctct ataagccagc agcagccgta
    15841 gcatccgcca gaagagccgg aaccaccgca accttagatg tactcacaaa atacttctcc
    15901 tatcaccaga tgcctgttgt atcatcctgc tactggaaca tggtacacgg aaacacaccg
    15961 gaagaagtaa tgaaagacga agaaggcgtt cagatcatgc gtgtccttgg aaaaaacatg
    16021 gcatggctgt taaagtgtat tgaagcagga aagaaaaacg gtgtagctgt tcctacagca
    16081 gaagataaga ttaagactaa ttttatccgg taattgatca tatgaaaaat aagttcaggg
    16141 agatggaacc gatatggttc atctccccaa attaattgca gcacttttca attgaatgac
    16201 aaaaacaaaa aagcagaaac ccttggcatt ccaaaagctt ccaccctttt ctaagctgct
    16261 gacgggaatc ggacccgtga cctccgcact accaatgcga cgctctaccg actgagccac
    16321 agcagcaact atatgaaatt tgccggttct gatgagatga aaagctgctg acgggaatcg
    16381 gacccgtgac ctccgcacta ccaatgcgac gctctaccga ctgagccaca gcagcgtttt
    16441 ctgttcgtca aaatcgaacc tcgtatatac tatcatgggg aatagtattt gtcaacggta
    16501 aaatgaaaaa aagttaaata ttttttatat cgtttttacg tactttaaga tcatctttgt
    16561 taattgctcc ggatccgctg gttttaacac ataatcatcc attccatact ggcggcactg
    16621 gctggaaaca ctgatggcat cctgggcagt catggcaagg atgggaatca tatttccgtc
    16681 aggacgggaa agggaacgta tctgcctgga agcttccagt ccgttcataa caggcatcat
    16741 cacatccata aggataatat cgtaggttcc ttccggtgac cgctcaaact gttccagagc
    16801 ctcttttccg ttccatgcct tatctaaggt ggcaccgcgg tctgtcaggt agaactctgc
    16861 gatctccata ttgatctcat tatcctctac taaaagtaca tggatccctt ccagttcttt
    16921 tccagtttcg gacgccgtat cagcctttgc atcatttgcc tcttcttttg caagtcggaa
    16981 aggaagacgg aaagtaaaag tagtgccttc tccagggctg ctctctactt ctatcgaacc
    17041 attcatctgc cggatcagac cctttacaat agacattcca aggccggttc ccttgtactg
    17101 tgtgcgggca tctgattttt cctgtgaaaa tggctcgaaa agctggttct ttaaaaatgc
    17161 ttcgctcatg ccgatgccag tgtcggaaat gaaaaattca taaacgacgg ttgttccgtc
    17221 aaaagagatc tcttttgcgc cggtatcaat aaaaccgcct tttttattat acttgatggc
    17281 attgctgaaa agatttaaca tgatctggcg cagctgcagg ggatagccgg agaggagcac
    17341 atgctggata ttttgccggt gtgtccggtg tgtcagtccc atttccatga tctgggcttt
    17401 taccagatag gaaacatccc gcatcagttg ggataagtca aaatctacag gcttaaaaac
    17461 agcctgtctc gactctaact tgctcatatc taaaacgtca tttaccagag caaggagatg
    17521 gctggaagaa acgtagatct tctctaagct gtcatctacc cgcgcgtggt cctccctgtt
    17581 tttgcgtatg atctcaagca ttcccataat accgttgata ggggtgcgga tgtcatgact
    17641 catacggttt aaaaactcgg tttttgcttt actggcagag tcggcttcct caaaggctac
    17701 ctggagccgt tctctttcct gctgttcttt ttcatggaag acagaagtgt ttttaaacag
    17761 gatcatacca tggatcacag tgctttcttt tccggtttca ctgttataaa ccgtatcctg
    17821 tgacataagc actgtttttt gcagccagaa aatttctccg ttttcatttt gttccctgta
    17881 ttctacggta acctgcttgg taccggagcg gaaacgctct aaaagtttcg cagaagagtc
    17941 cacaatgcgg taattttcct gggtatcttc cgtaacaaaa cgactgcggt ttaaacagta
    18001 ttcatcataa gaacagggaa attttatgtt caggtcaaat tcgcattccg ttgtgtgata
    18061 gaagattttt tccaaccggt cccctgtcag gtctacagaa tagacagcct gcgca
//
LOCUS       DBMDUA010000037        18088 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1838_length_18088_cov_2.206705, whole genome shotgun sequence.
ACCESSION   DBMDUA010000037 DBMDUA010000000
VERSION     DBMDUA010000037.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 18088)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18088)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18088
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1838_length_18088_cov_2.206705"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(217..804)
                     /gene="lepB"
                     /locus_tag="ACZPCL_04695"
     CDS             complement(217..804)
                     /gene="lepB"
                     /locus_tag="ACZPCL_04695"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR1161808.1"
                     /translation="MSEEEKKGRNRRNSEEEPQEETSLGKEILSWLQIIVAAVVIAFV
                     LNNFVIANSRIPTGSMENTIMSHSRVIGSRLSYIKDDPERGDIVIFRYPDNEKVYYVK
                     RVIGLPGETVEVKDGKVYINGSDTPLDEPYLPEPMEGSYGPYEVPEGCYFMMGDNRNN
                     SLDARFWDNKFVAKNKIVAKVLFCYYPFNRIGKVN"
     gene            complement(837..2078)
                     /locus_tag="ACZPCL_04700"
     CDS             complement(837..2078)
                     /locus_tag="ACZPCL_04700"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR1161809.1"
                     /translation="MTLEEIGSRAKAVSRTLNNLGSREKNIGLEEAARALLEGEDEIL
                     AANQADYEKAESSGMSKGMLDRLALNPGRIQSMADGLLKVAALDDPVGEVISMKLRPN
                     GLKIGQKRVPLGVIAMIYEARPNVTADAFGLCFKSGNAVILKGGSDAIESNKAIAKWL
                     RTGLTRAGLPEDALQLIEDTSRETTKELMRLNRYVDVLIPRGGAGLIRSVVENSTVPV
                     IETGTGNCHIFVDETADFNMALDIIYNAKTQRIGVCNACESLIVHRKIADQFLPLLKK
                     RLAEKEVEVRADEAARAVVPEFTPATEEDWGTEYLDYILSLKIVDSVEEAIEHINKYN
                     TGHSESIITSDYFNAQKFLDEVDAAAVYVNASTRFTDGEEFGFGAEIGISTQKLHARG
                     PMGLKELTTTKYIIYGDGQIR"
     gene            complement(2130..2351)
                     /locus_tag="ACZPCL_04705"
     CDS             complement(2130..2351)
                     /locus_tag="ACZPCL_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HIR1161810.1"
                     /translation="MDSSKIDRINELYHKSQSVGLTEEEKTEQAALKKEYLAAIRANL
                     RSSLNNISIKEKDGSITDLGKKYGGIKEV"
     gene            complement(2396..3259)
                     /gene="proB"
                     /locus_tag="ACZPCL_04710"
     CDS             complement(2396..3259)
                     /gene="proB"
                     /locus_tag="ACZPCL_04710"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR1161811.1"
                     /translation="MEATKRNSLKDKKRIVIKIGSSSLTHPETGELNLTKIEKLIRVL
                     SDLRGEGKEVVLVSSGAIAAGRQAFGHHKRPDSLAEKQAFAAVGQARLMMVYQKLCSE
                     YNQTAAQVLLTKDTMVNDSSRYNAQNTFDELLRLGAIPVVNENDTVSTSEIPYVDSFG
                     DNDRLSAIVAALIGADLLILLSDIDGLYTDDPRSNPDAKFISLVPEISQKYLNMGKET
                     SGSSVGTGGMSAKLAAARIATDSGADMVIANGDQVDVIMDIMSGKEKGTLFLAHPNLD
                     FDLMHYLNNEY"
     gene            complement(3462..5090)
                     /locus_tag="ACZPCL_04715"
     CDS             complement(3462..5090)
                     /locus_tag="ACZPCL_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1161812.1"
                     /translation="MIRISQLKMPLGHTRKDLLLKAAKELRIQPEMIKEVELIKQSVD
                     ARKKPEIHYSYIVDIALKSGGRKEEERIVKKKKDRNITVHEEKPYSLPECGNEPLKYP
                     PVIIGMGPAGLFCGLALARKGYRPILLERGEDVDTRTERVAKFWKTGELNPSSNVQFG
                     EGGAGTFSDGKLNTLVKDTFGRNREVLRILTEFGADPSICYANKPHIGTDVLSHIVKS
                     IRKEIESLGGQVRFLSQVTDFEITEGKLTAVTVNNTERIPVQAAVAAIGHSARDTFET
                     LYGKQVSMEAKPFAVGFRIQHPQTMINESQYGMAECKELGPASYKLTAQTSYGRGVYS
                     FCMCPGGYVVNASSEEKRLAVNGMSYHDRAGENANSALIVTVNPEDFSHDIKGREEVH
                     PLAGIHFQRELEEKAYEAGNGKIPVQLYGDFKDGKISEKFGKVHPAFCGEYQFADLRK
                     ILTNELSEAFIEGMESFGKKIKGFDRPDAILAGVESRTSSPVRIPRGETLESSVRGLY
                     PCGEGAGYAGGITSAAMDGLKVAEELIRRYKLTE"
     gene            complement(5087..6463)
                     /locus_tag="ACZPCL_04720"
     CDS             complement(5087..6463)
                     /locus_tag="ACZPCL_04720"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BaiN/RdsA family NAD(P)/FAD-dependent
                     oxidoreductase"
                     /protein_id="HIR1161813.1"
                     /translation="MKKRVGIIGGGASGLVAAIGAARNGAEVTVIEHMDRVGKKILST
                     GNGRCNLTNYKMEEDCYRCGRKDFPMQVIRGFDVKETLDLFMGLGIVPKDRNGYVYPN
                     SDQAASVLDVLRTELERLKVRILLSCQIEKIEKKKNGGFLVHTDQGKVETDALILAAG
                     SKAAPSTGSDGSGYEYARQLGHSVIKPLPALVQLRCQGNLYKQMAGIRTEAEVRLQAD
                     GITLASDRGELQITDYGLSGIPIFQVSRYAARALDEKKKVRVYVDFMPGWDDNESFRL
                     LKKRALLLAYKPAEELFTGMLNRKLAQALLKLAGIDPNTPCGSLTGKQLEKIRKELKE
                     YEAVVMSVNPFANAQVCCGGVDANEVDGTTMESKICPGLYLAGEILDVDGICGGYNLQ
                     FAWSSGMIAGRCAAGNAEKKSIEKKSIEKKNIEKKRNINKKPMEKKPVKKYAEKKYTQ
                     KTRRTART"
     gene            complement(6481..6861)
                     /locus_tag="ACZPCL_04725"
     CDS             complement(6481..6861)
                     /locus_tag="ACZPCL_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161814.1"
                     /translation="MKHFLFALGLFSISAVVSVLVIYGAARYQMREEAPVPNTVIETE
                     SVNEMEGRAAANQESITPVSSRYFLVSESGFLLVFCSDKSTVCLYTHIPVTDFPESER
                     ARLMEGIWFSDMMDVYHYLESYTS"
     gene            complement(6974..8689)
                     /locus_tag="ACZPCL_04730"
     CDS             complement(6974..8689)
                     /locus_tag="ACZPCL_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative polysaccharide biosynthesis protein"
                     /protein_id="HIR1161815.1"
                     /translation="MKAVQGKKKNKAANFVIQGSILAMAGILVRIIGMLYRMPLNDII
                     GKQGNGYYTSAYNVYNILLILSSYSMPVAISKMISARLAKGEYENCRRILKAGLLYAT
                     VTGGLAAIILWFGSDFFAGLIKTPYSSYALKTLAPTIWIMAYLGVLRGYFQGTGTMIP
                     TAVSQILEQIVNAIVSVVAAGLLFHVGEGMNAAQGAKNYSYALGAAGGTIGTGAGALT
                     AFIFFIFLTAKSGRSVLKQRIATEELSEEAAKYARRKRTRYRKLIYALTITVLPIVVS
                     SGIYNCSNVVDNFLFGQGMDKLGYSGEEIAVFWGALGQYQLLFNIPVAVSNALSSSLI
                     PSLTRAVSAGDREQTLKRIAAAVRFSMLISIPAAVGIAVLADPVCNLLFISEDNSLLI
                     KLTMAGSLAVVFFSLSTVTNAVLQGLNRMEVPVRHAAVSLVIHVAVLWVLLMVFHMGI
                     YGVVFANIVFAFVMCVLNARTIAKHARYRQEYKKTFVLPAISACVMGGAAWGVYKVLT
                     FILPAALLRGRSGRAIVVFPAVAVAAAVYAVLLVGLKAIDEEELRDMPMGTKMIRILK
                     KVRLL"
     gene            complement(8744..9925)
                     /locus_tag="ACZPCL_04735"
     CDS             complement(8744..9925)
                     /locus_tag="ACZPCL_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HIR1161816.1"
                     /translation="MKKGSRSKKAVRRKMMLGTILCIAALCAGCGKKASEEAAQTQVP
                     TTQETTTQAATSAPEILIQSDEEQKEETKDLPDYNLPEEREEKDGMIRSFLTGQMVPV
                     EQANRRPIAIMMSNDKEARPQYGMNRAGVVYEAPVEAEMNRYMAVIEDYDDLDRIGSV
                     RSCRTYYTYFAREFDAIYAHFGQSTFAKPYLNNVDNINGLDAIGNTTYYRSKDKHSPH
                     NAYTSGDRLKKAIATLGYSTEYDESYTGHYLFARDGVEISLDGKPGVIDAAHAKPGYT
                     MNKPDFVYDSSDGLYHRYQYGDVHRGSEGPVAVKNVIFQYCQSGFYASTQYLNINVHT
                     GEYGYYITDGKAIPITWEKDGEFGVTHYYDHDHNEIVLNQGKTWVCIIPTKDFDKSEI
                     K"
     gene            complement(9922..10419)
                     /gene="ybeY"
                     /locus_tag="ACZPCL_04740"
     CDS             complement(9922..10419)
                     /gene="ybeY"
                     /locus_tag="ACZPCL_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR1161817.1"
                     /translation="MTVSIEYEAEKKLDLPYEKIIKEIVEASLDFEKCPYEAEVNVIL
                     TDNDAIQAINKEYRQIDAPTDVLSFPMVDYEKPSDFDHVEDAVEDYFNPETGELMLGD
                     IVISVDKVEEQAEKYGHSQTRELAFLVAHSMLHLCGYDHMVDEERLVMEDRQRQILDT
                     RGYTR"
     gene            complement(10419..11447)
                     /locus_tag="ACZPCL_04745"
     CDS             complement(10419..11447)
                     /locus_tag="ACZPCL_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR1161818.1"
                     /translation="MSVIETMIDIPAEHERNVCGQFDAYLKKIERTLHVTMIARDGEI
                     KLIGPEKTIGQAKSVFNNLIQLSMRGNTITEQNVDYALSLSFTENDDQILEIDKDIIC
                     RTIMGKPVKPKTLGQKHYVDSIRKKMIVFGIGPAGTGKTYLAMAMAIQAFKNGEVGRI
                     ILTRPAIEAGEKLGFLPGDLQSKIDPYLRPLYDALYQIMGAESYQHNSEKGLIEVAPL
                     AYMRGRTLDNAYIILDEAQNTTPAQMKMFLTRIGFGSKVIITGDQTQKDLPSGATSGL
                     DTALKVLKNIDDIGFCYLTSSDVVRHPLVQKIVKAYDDYEKKTASKTARGERKTPAVK
                     NTRKGNNR"
     gene            complement(11456..11746)
                     /locus_tag="ACZPCL_04750"
     CDS             complement(11456..11746)
                     /locus_tag="ACZPCL_04750"
                     /inference="COORDINATES: protein motif:HMM:NF018591.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HIR1161819.1"
                     /translation="MIQLKLFSNFYLPLFVGKIAGEYMTVYEKGYTKEELEQTASETN
                     RQFVEKLREKGVQILENNDKIEKNISGYRISGTLKVIEPAAVSVPVSKTQGD"
     assembly_gap    11853..11862
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11863..12780)
                     /locus_tag="ACZPCL_04755"
     CDS             complement(<11863..12780)
                     /locus_tag="ACZPCL_04755"
                     /inference="COORDINATES: protein motif:HMM:NF018591.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HIR1161820.1"
                     /translation="MIRYFFRLLKGYVRIQIKSISPEYFLERCRINGIFFWELVCLKK
                     GEYQCSLFLKDFYRLRGCARGKGVRLRICERRGLPFFLQRNRKRWYAAAGLSSFFLLL
                     YIMSLFIWDIHFQGNHHYTADTLVRYLDTQDVRYGMKKKKINCEELEAGMREAFPQIT
                     WVSARVSGTRLVIQIKENKIIQAKTTVKEDPCDLVSDVTGTITYIMVRQGIPKVKKGE
                     EIQQGQVLVSGRVPVIGDDEQEISAFYVQSQGEILAETVETYEKELPLTRDVRYPTGR
                     KRYGLRLIAAPVLTMVMGPKETVVFVIIAG"
     gene            complement(12777..13073)
                     /locus_tag="ACZPCL_04760"
     CDS             complement(12777..13073)
                     /locus_tag="ACZPCL_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HIR1161821.1"
                     /translation="MREDRKRAAVEALGLPRDVILGDVLLHFTGPYEVVAENFRSLLI
                     YTDTYVKIKAAGCMVVFKGLHLEIETYTEDALKIKGQIRSVEFDDLLSERRVEG"
     gene            13213..14199
                     /locus_tag="ACZPCL_04765"
     CDS             13213..14199
                     /locus_tag="ACZPCL_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HIR1161822.1"
                     /translation="MTNSKLNPASEAISMVKAAQTNEEDVPVTPLPDPGEGGPVYDGS
                     MNNDPMDNGSGGNGSFGDDLSSVPVIPLPNPGEGGPVYNGPNYNRPSGGMGQPGNSQI
                     PVYRPVFPSNGSITVLPGISFPCYNCTTSSSLGRVRILNASSGYPAFNVSLGNWRIGE
                     QLDNGDLTSYVQAASGFQTVTVSGTNGYIYIQKMITIRAGSASTVAIINTSTGLDLME
                     ISDLSCNGPSGTACIRACNLSPDLGPFDVALENRGNSYRTFTNVRFQEVTPFSSFASG
                     WYSIYVFRTNAFNNNNAIAAASAALKTGGSYTLYMFNGPSSTDGLRTLILSN"
     gene            complement(14509..15663)
                     /locus_tag="ACZPCL_04770"
     CDS             complement(14509..15663)
                     /locus_tag="ACZPCL_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HIR1161823.1"
                     /translation="MKYAVICFTERGAWVCRRLFHKLKETGEECEAIIPKRFLREEWK
                     KEGLKEREEESLSQWTGKMFAEKRAMIFVSATGIAVRAIAPWIRDKMTDPPVVTVDEG
                     AQFVIPLLSGHVGGANELARHIADWLEAVPVITTATDVNGKFAVDLFASAYHMTITDR
                     KEAKNISAAVLEGKQIGVFSDLPIKKLPDGFVMDRWCEENICITVKDPSFPEKKASYL
                     RLVPRAVVLGVGCRRGTDPEFMKEKVFALLKEHGIDPAAVKAIASVDVKQDEPAVLDL
                     KQLFDGECLHQPCEQRFYTPEQLNQVPGDFKESAFVKKQIGVGNVCERSACAAGGKLL
                     VEKQAGDGITLAAALEWQGELDFSKGFGTTVRGKECLWDRNTQFYILKKI"
     gene            complement(15764..15976)
                     /locus_tag="ACZPCL_04775"
     CDS             complement(15764..15976)
                     /locus_tag="ACZPCL_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HIR1161824.1"
                     /translation="MGKSKKKKEFDVHNMTPDEQLKFEIAQELGLDEKVLQKGWKSLT
                     SKESGRIGGMITGRKRQLKEEALKES"
     gene            complement(16158..>18088)
                     /locus_tag="ACZPCL_04780"
     CDS             complement(16158..>18088)
                     /locus_tag="ACZPCL_04780"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HIR1161825.1"
                     /translation="VERAVARVYEQIQAVKEGKAQGPIYTYGPIIHNEEVVRDLEEKD
                     VQVLDTEEDLENLPEGQGTVIIRSHGVSRHVYDILNSRGVNVVDATCPFVKKIHKIVD
                     EHSRAGEAVIIIGSPVHPEVEGIRGWGNGDTVVVENEADAEDYNLPEGKKLCIVSQTT
                     FNYNKFQELVEKISKKGYDSSVLNTICNATQERQVEAARIASSVEAMIVIGGRHSSNT
                     QKLYEICLKECKNTFYIQTLGDLDPDSVSSVRSVGITAGASTPKKIIEEVHTRMAEQS
                     FAELLKEEEESTKPIRTGEIVTGQVIEVNKDEIILSIAGNKSEGVITRDEYSNDNSVD
                     LTTKVQVGEEMEAKVIKLNDGVGMVSLSYKRMAADRGNKRLEEAFENQEVLTAKVAQV
                     LDGGLSVEVEGSRVFIPASLVSDTYEKDLSKYAGQEIQFVITEFNPKRRRIIGDRKQL
                     LVTEKAKLKEELFSKIQVGDKVEGTVKNVTDFGAFIDLGGADGLLHISEMSWGRVENP
                     KKVFKSGDKVTVLIKDIQGEKIALSLKFPEANPWAHAAEKYAAGSVVTGRVARMTDFG
                     AFVELEPGVDALLHVSQISREHIEKPADVLKIGQEIEAKVVDFNEDDHKISLSVKALQ
                     AAPQNEDVADVDIDSYAK"
BASE COUNT         4442 a         4668 c         3426 g         5542 t
ORIGIN      
        1 tcttctaaaa tgtgtaactg ttcctttgcg tgagccataa aatactgctg acgctgtggt
       61 tcctgttccg gtatctccgt cataaatggg gtcttatttt ccatatagat ctcctttaaa
      121 ttcacctttc aattatctaa aaatcttctt tatctaaaac acacaagcag gccgggcaga
      181 atatcaccca gccagttgta tctttgcttt ctgtaattag tttacttttc ctatcctgtt
      241 aaatggataa taacagaaca gcacttttgc cacgatcttg ttcttggcaa cgaatttatt
      301 atcccagaaa cgggcatcca gggaattatt ccggttgtcg cccatcatga aataacagcc
      361 ctccggcact tcataaggtc cgtaggaacc ttccattggc tccggcagat aaggctcatc
      421 aagaggtgta tcagaaccat tgatatagac ctttccatcc ttcacctcaa cggtctctcc
      481 cggaagtcca atgactctct ttacataata tactttttca ttgtccgggt aacggaagat
      541 cacaatatct cctctttccg gatcatcttt tatataagac aatctggagc cgatcacacg
      601 gctgtgggac ataatggtat tttccatgga tccggttggg atgcggctgt tggcaatgac
      661 aaaattattt aaaacaaaag caatgactac cgcagccaca atgatctgaa gccagcttaa
      721 gatctctttt cccagactgg tctcctcctg cggctcttcc tcggaatttc tccgattgcg
      781 gcctttcttt tcttcttctg acatgttggt ggtttcctct tctttctttt ctatacctaa
      841 cggatctggc catcaccata gatgatatac ttggtggtag tcagttcttt taagcccata
      901 gggcctctgg cgtgaagctt ctgggtactg ataccgatct ctgccccgaa gccgaattct
      961 tctccgtctg taaaacgggt ggatgcattt acatacactg ctgcagcatc tacttcgtct
     1021 aagaacttct gggcattaaa ataatccgat gtgataatag actcagaatg tccggtattg
     1081 tatttattga tatgttcaat ggcctcttct acagagtcta caatctttaa agataagata
     1141 tagtccagat attctgtacc ccagtcttct tctgttgcag gagtaaattc cggtacaacc
     1201 gctctggctg cctcgtctgc tcttacttct acctcttttt ctgccagacg ctttttaaga
     1261 agtggaagga actggtccgc aatctttctg tggacgatca gggactcgca ggcattgcat
     1321 acaccaatgc gctgtgtttt tgcgttatag ataatatcta atgccatatt aaaatcagcc
     1381 gtctcatcta caaaaatatg gcagtttcca gtacctgtct cgatcacagg aacggtgctg
     1441 ttttccacta ccgaacggat cagtcctgcg ccgcctcttg ggatcagcac atccacatag
     1501 cggtttaaac gcatcagttc ttttgtagtc tctctgctgg tatcttcaat cagctgcagg
     1561 gcatcctctg gaagtccggc tctggtaaga ccagtgcgca gccattttgc gatcgcttta
     1621 ttagactcaa tggcatcgct gccgcctttt aagatcactg catttccaga tttaaagcag
     1681 aggccgaaag cgtccgctgt tacattagga cgggcctcat agatcatggc aatgacacct
     1741 aaaggcaccc tcttctggcc gatctttaac ccatttggac gcagtttcat ggagatcact
     1801 tctcctaccg gatcatctaa ggcagctact tttaaaaggc cgtctgccat agactggatc
     1861 cgtcccggat ttaaagccag acggtctaac atgcctttgc tcatgccgga actttctgct
     1921 ttttcataat ctgcctggtt cgctgccagg atctcatctt ctccctctaa cagggctctg
     1981 gctgcttctt ccagtcctat atttttctct cttgaaccta agttatttaa tgttctggaa
     2041 acagcttttg cgcggcttcc gatctcttcc agtgtcataa ccatctctcc ttctggctat
     2101 aaatatactc tcggattttt ttatactgct tatacttcct tgatgccacc gtattttttg
     2161 cccaggtctg taatagaacc atctttctct ttaatagaga tattatttaa actgcttctt
     2221 aaattcgcac ggatggcagc cagatattcc ttttttaaag ctgcctgctc ggttttttcc
     2281 tcctctgtaa ggcctacaga ctgggattta tgataaagtt cgttaattct gtcgatcttg
     2341 cttgagtcca taattttact ccttaatgat attgctgtag ttctgccaca gcattttaat
     2401 actcgttatt cagataatgc atcagatcaa agtccagatt aggatgagct aaaaataaag
     2461 ttcccttttc ttttccactc ataatgtcca tgatcacatc tacctggtca ccattggcga
     2521 tcaccatatc agctccgctg tctgtggcaa tgcgggctgc tgccagtttt gctgacattc
     2581 cgccggttcc cacactgctt ccggaagtct cttttcccat atttaagtac ttctggctga
     2641 tctccggcac cagactgata aatttagcgt ccggattgct tcttggatca tcggtataaa
     2701 ggccgtcaat atcagaaagc aggatcagaa ggtctgctcc gatgagagct gcaacaatag
     2761 cagaaaggcg gtcgttatca ccgaaactgt ccacataagg gatctcagag gtagataccg
     2821 tatcattttc atttacaaca gggattgcac ctaaacgcag cagttcatca aaggtgttct
     2881 gtgcgttata gcgggaactg tcattgacca tagtatcctt tgtcaaaagg acctgggccg
     2941 ctgtctggtt atactcactg caaagcttct ggtagaccat catcagtctg gcctgtccta
     3001 ctgctgcaaa agcctgtttt tccgctaaac tgtccggtct tttatgatgt ccaaaagcct
     3061 gtctgcctgc tgcaatagca ccggaagaaa caaggactac ctctttgcct tcgcccctca
     3121 ggtcgctgag cacacggatc agcttctcaa tctttgttaa attcagttct cccgtttccg
     3181 gatgggtcag ggaagaggaa ccgattttta ttacaattct ttttttatct tttaaactgt
     3241 tacgttttgt agcttccata ggtatcttct gtcagctccc ttctgcatag gtttcgtcct
     3301 gcctatcttc ttggcttcat gttccatgaa accaagaagc atgctttgtc aagcatatgc
     3361 tgatttctgc aataagctgt ttctgcaagc aagcttgcaa cacatcttat cacataaatc
     3421 agcgcaggac tggacttttc cactatctta caccaatttt attactccgt caatttgtat
     3481 cttctgatca gttcttccgc taccttaaga ccatccatag ctgcggaagt aataccgcct
     3541 gcatatccgg cgccttcccc gcagggatat aaacccctga cagaactttc taatgtctct
     3601 cctcttggaa tacgcacagg agaggaggta cggctttcca cacctgctaa aattgcatcc
     3661 gggcggtcaa atcctttgat ctttttgcca aaactttcca ttccctctat gaatgcttca
     3721 gaaagttcgt ttgtgaggat ttttctcagg tctgcaaatt gatattctcc gcagaaagcc
     3781 gggtgaactt tgccaaattt ttctgagatt tttccgtctt taaaatctcc atacagctga
     3841 accggtattt ttccatttcc tgcctcatat gccttttcct caagctctct ctggaaatga
     3901 atacctgcca gaggatgtac ctcttcccgg cctttaatat cgtgagaaaa atcctcagga
     3961 tttactgtga caatcagagc actgttggca ttctcccctg ctctgtcatg atagctcatt
     4021 ccatttactg ccagacgttt ctcctcagaa gaagcattaa ctacatagcc gccggggcac
     4081 atacagaaag aatacacacc tcttccataa gaagtctgtg ctgtcagctt atagcttgca
     4141 ggtcctaatt ctttgcattc tgccattcca tactggcttt cattgatcat ggtctgtgga
     4201 tgctggatac ggaaacctac agcaaatggc tttgcttcca tagatacctg ttttccataa
     4261 agagtttcaa aggtatctct ggcgctgtgg ccgatcgctg caactgctgc ctgtaccggg
     4321 atccgttctg tattatttac agtaaccgcc gtcagtttcc cttctgttat ctcaaaatct
     4381 gttacctggc ttaagaaacg gacctgtcct cccaggcttt cgatctcttt tcggatagat
     4441 tttacaatat ggcttaacac atccgttcca atatgaggtt tattggcata gcagatggac
     4501 gggtctgctc caaactctgt caggatacgt aaaacttccc tgttccttcc aaaggtatct
     4561 tttacaaggg tgttcagctt tccgtcggag aaagtacctg caccaccttc tccaaactgt
     4621 acgttagatg aagggttcaa ctctcccgtt ttccagaatt ttgccacccg ctctgttctg
     4681 gtatctacat cttctccccg ttctaaaagg atgggacggt acccctttct tgcaagggca
     4741 aggccacaaa acagtcctgc aggtcccatt ccaatgatca caggaggata ttttaaaggc
     4801 tcatttccac attcaggaag agaatagggc ttttcctcat gaactgtaat atttctgtcc
     4861 tttttctttt ttacaatgcg ctcctcttct tttctgccac cgctttttaa agcaatatct
     4921 acaatataac tataatggat ctccggcttt tttcttgcgt ccacagactg tttgatcagt
     4981 tctacttctt ttatcatctc aggctggata cgaagttctt ttgccgcctt taaaagcagg
     5041 tcctttctgg tatgtcccag aggcattttt aactgactga tacgtatcat gttcttgcag
     5101 tcctcctggt tttctgtgta tatttcttct ctgcgtactt tttaacaggt ttcttttcca
     5161 tcggtttctt atttatgttt ctcttttttt ctatattttt cttttctatg cttttcttct
     5221 ctatactctt cttctcggca tttcccgctg cacatcttcc tgcgatcatg ccgctggacc
     5281 aggcaaactg aagattgtag ccaccgcaga taccatccac atccaggatc tcccctgcca
     5341 gataaagccc cggacagatc tttgactcca tagtagttcc atccacctca ttagcatcta
     5401 caccgccgca gcacacctgg gcattggcaa aaggattaac ggacatgaca actgcttcat
     5461 attcttttaa ttctttgcgg atcttttcca actgttttcc agttaaagag ccacagggcg
     5521 tatttggatc aatgcctgcc agtttcaaaa gagcctgggc aagtttgcgg tttaacattc
     5581 ctgtaaacag ttcttctgcc ggtttgtaag ccagcaaaag ggctcttttt tttaaaagcc
     5641 ggaaactttc attatcatcc cagcccggca taaagtccac ataaactctt acctttttct
     5701 tttcatctaa cgcccttgcg gcataacggc ttacctggaa gataggaata ccggaaagac
     5761 cgtaatctgt gatctgcagc tctcctctgt cacttgccag ggtaataccg tctgcctgca
     5821 atctcacttc tgcttccgtg cggatccctg ccatctgttt gtataaattt ccctgacagc
     5881 gaagctggac cagagccgga agtggcttga tcacagaatg tcctaactgt ctggcatatt
     5941 cgtaaccact gccatctgaa cctgtagaag gcgctgcttt ggaaccagcc gcaaggatca
     6001 gggcatctgt ttctaccttt ccctgatctg tatgtacaag aaagccaccg tttttctttt
     6061 tttcgatctt ttcgatctga caggaaagga ggatacggac tttcagtctc tctaattctg
     6121 tccttagcac atctaaaaca gaagcagcct ggtcggaatt aggataaaca taaccgtttc
     6181 tgtcttttgg aacgatcccc agtcccataa agagatccag cgtctctttt acatcaaagc
     6241 cccttattac ctgcatagga aaatccttcc tgccgcagcg gtagcagtct tcttccattt
     6301 tgtaattggt aagattacac ctgccgttgc ctgtagaaag gatctttttt cccacgcggt
     6361 ccatatgttc tatgacagtc acttccgccc cgtttcgggc tgctcctata gcagccacca
     6421 gcccggatgc gccaccgcca ataattccta ctcttttctt catatttttt aactcctgtt
     6481 ttaacttgta tacgactcca gataatggta tacgtccatc atatctgaaa accagatgcc
     6541 ttccatcagt cgggctcttt cactttccgg gaaatcagtt accggaatat gggtgtacag
     6601 acagacggtg cttttatcac tgcaaaatac aagaaggaaa ccggactcac tgacgagaaa
     6661 ataccgggaa cttaccggag taatgctctc ctggttggca gctgcccttc cttccatttc
     6721 attcacactt tctgtttcga tcactgtatt aggtaccggc gcttcttccc tcatctggta
     6781 acgtgccgcc ccgtagatca caagaaccga tactacagct gatatactga acagtcccag
     6841 ggcaaataaa aaatgtttca tggatactac ctcctgtatc aagtcatata cgttcattta
     6901 ctgacctttt cagaaatagt atctgccata atagaccatt ttaaacatgt cctggcactg
     6961 tttgcaatac catttacagt aatcttactt tctttaaaat gcggatcatt tttgttccca
     7021 taggcatatc ccgcagttct tcttcatcaa ttgccttaag tcccactaaa agtactgcat
     7081 aaacagcagc tgctactgcc actgccggga ataccacgat cgctctgccg gaacgtcctc
     7141 ttagaagcgc tgccggaagg ataaaggtaa gaaccttgta tacaccccag gctgccccgc
     7201 ccataacgca ggcgctgatc gccggaagta caaatgtttt cttatactcc tgacgatatc
     7261 ttgcatgctt tgcaatagta cgggcattta aaacgcacat tacaaaggcg aatacaatgt
     7321 ttgcaaatac aacaccataa atacccatat ggaataccat aagaagtacc caaagcactg
     7381 ccacatggat caccagagaa acagctgcat ggcgtaccgg cacttccata cggtttaatc
     7441 cctgaagcac tgcattggtc actgtggaca gagagaagaa tacaaccgca agagaacctg
     7501 ccatagtcag cttaattaaa agactgttat cttcgctgat aaacagcaga ttgcacacag
     7561 gatctgctaa aaccgcaata cctactgctg ccgggataga gatcagcata gagaaacgta
     7621 ctgctgctgc gatccttttt aaagtctgtt ccctgtctcc tgcagatacc gctcttgtaa
     7681 gagacgggat cagagaggag gaaagggcat tagaaacagc taccggaata ttaaataaca
     7741 gctgatactg tcctaaagct ccccagaata cagcgatctc ttccccgctg tatcctaact
     7801 tgtccattcc ctgtccaaag aggaagttgt ccactacatt ggaacaatta taaataccgc
     7861 tgctgactac aatagggagt actgtgatcg tcagggcata gatcagtttt ctatatcgtg
     7921 tccgctttct tcttgcatat ttcgctgctt cttctgataa ctcctctgtc gcaatacgct
     7981 gttttaaaac agaacgaccg ctttttgctg tcaggaagat aaagaagata aatgcagtaa
     8041 gagcgcctgc acctgtacca atggttccgc ctgcagcacc taacgcataa gaataattct
     8101 ttgctccctg ggccgcattc atcccttctc ctacgtggaa taaaagtccc gctgcaacta
     8161 cgctgacaat ggcattaacg atctgctcta agatctgtga aaccgcagta gggatcatgg
     8221 ttcctgttcc ctggaaataa cctcttaaaa ctcccagata cgccatgatc cagatggtag
     8281 gtgccagtgt ttttaaggcg taactggaat aaggcgtctt gatcagtcct gcaaagaagt
     8341 cagaaccaaa ccaaagaata atggctgcaa gtccgccggt aactgttgca tagagaaggc
     8401 ctgcctttaa gatgcggcgg cagttctcat attctccttt tgcaagcctt gcggatatca
     8461 tttttgagat agccaccggc atactgtaag aggaaaggat cagaagaatg ttgtatacat
     8521 tatacgcaga tgtataatat ccatttccct gctttccgat aatatcattt aaaggcatac
     8581 ggtaaagcat accgataata cgcaccagga taccggccat agccaggata cttccctgta
     8641 ttacgaaatt tgctgcctta tttttctttt ttccctgtac tgctttcatt tatcttctac
     8701 tgtgctccat gtttattact taaaacgtgt atcgccacgt tttttattta atttcagact
     8761 tatcaaagtc tttagtaggg atgatgcaga cccaggtctt tccctgattt aatacgatct
     8821 cattgtggtc atgatcgtaa taatgggtca caccaaattc cccgtccttc tcccaggtga
     8881 tagggatggc ttttccgtct gtgatataat acccatattc accggtatgt acattgatgt
     8941 tcagatactg ggtagaggca tagaaaccgc tctggcagta ctggaagatc acatttttca
     9001 ctgctacagg gccttcactt ccgcggtgta catccccata ctggtatctg tgatacaggc
     9061 catcactgct gtcatacaca aaatccggct tgttcatagt atatccaggt tttgcatggg
     9121 cagcatctat cacgcctggt tttccatcta aagagatctc cacgccatct ctggcaaaaa
     9181 gataatgccc cgtataagac tcatcatact ctgtactata gcctaaagtc gcaattgcct
     9241 ttttcagacg gtctccgctt gtgtaggcat tgtgaggaga atgtttatcc ttgcttctgt
     9301 aatacgtagt atttccaata gcatccagac cattgatatt atccacatta ttaagataag
     9361 gctttgcaaa ggtactctgg ccaaaatggg catagatcgc atcaaactct cttgcaaaat
     9421 aagtataata ggtacggcag cttctcactg aaccaatgcg gtccagatca tcataatcct
     9481 cgatcactgc catatagcgg ttcatttccg cttccaccgg tgcctcatag accacaccgg
     9541 cccggttcat accgtactga ggtcttgcct ctttgtcatt gctcatcatg atagcgatag
     9601 gacggcggtt tgcctgttct accggtacca tctgacctgt aaggaagctt cggatcatgc
     9661 cgtctttttc ttcccgttct tctggtaagt tatagtccgg caaatctttc gtttcttcct
     9721 tttgttcctc atccgactgg atcaggattt cgggagcaga agtggctgcc tgggtggttg
     9781 tttcctgtgt tgtgggaacc tgcgtctgtg ctgcttcttc tgacgctttc ttcccgcatc
     9841 ctgcgcacag cgccgctatg caaaggatcg ttcctaacat cattttcctt cgtactgcct
     9901 ttttacttct gctccctttt ttcatctggt gtaccccctt gtgtccagta tctgtctctg
     9961 acggtcttcc ataaccagcc gctcttcatc taccatgtgg tcatatccac ataaatgaag
    10021 catactgtgc gctactaaaa atgctaattc ccttgtctgg gaatgaccgt acttttcagc
    10081 ctgttcctcc accttgtcta cagagatcac gatgtccccc aacatcagtt ctcctgtttc
    10141 cggattgaaa taatcttcca ctgcatcttc cacatggtca aaatcagaag gtttctcata
    10201 atcaaccatt ggaaaagaca acacatccgt aggcgcatcg atctgacggt actctttatt
    10261 gatggcctgg atggcatcat tgtctgtcag aatcacattc acctctgcct cataagggca
    10321 tttttcaaaa tcaagagacg cctctacgat ctcctttatg atcttctcat agggaagatc
    10381 cagttttttc tctgcttcat actcaatgga aactgtcatt accgattatt cccctttcgt
    10441 gtatttttca ctgccggagt ttttctttcc cctctggcgg tctttgacgc tgttttcttc
    10501 tcataatcat catatgcctt gacgatcttc tgtaccagag gatgacggac tacgtcagaa
    10561 ctggtcagat agcagaaacc aatatcgtca atgtttttaa ggacttttaa agctgtatcc
    10621 agaccggaag ttgcaccgga aggaaggtct ttctgggtct ggtctccggt gatgatgacc
    10681 ttggagccaa aaccaattct tgttaagaac atcttcatct gggccggtgt ggtattctgt
    10741 gcctcatcca ggatgatata tgcattgtcc agggtacgtc ctctcatgta tgcaagaggt
    10801 gctacctcaa taagaccctt ttcggaatta tgctgataac tttccgctcc catgatctga
    10861 tataaggcat catataacgg gcgcagataa gggtcgatct tactctgtaa gtcacctgga
    10921 agaaaaccta acttttctcc tgcctcaatc gcaggtcttg taaggatgat acggcctact
    10981 tccccgtttt taaaagcctg gatggccata gccatggcaa gataggtctt accggtacca
    11041 gccggtccaa tgccgaatac gatcatcttc ttgcggatgg agtccacata atgcttctgg
    11101 cctaaagtct ttggctttac cggctttccc ataatggtac ggcagataat gtccttatcg
    11161 atctccaaga tctggtcatc attttccgta aaggaaagag acagggcata gtccacattc
    11221 tgctcggtaa tagtattccc ccgcatggaa agctggatca ggttattaaa tacgcttttt
    11281 gcctgaccaa tggtcttttc cggaccgatc agcttgatct cgccgtccct ggcgatcatg
    11341 gtcacatgaa gggtccgttc tattttcttt aaatatgcat cgaactgccc gcagacattt
    11401 ctctcatgtt ctgccgggat gtcgatcatg gtttcaatta cactcatccg tctgtttaat
    11461 ctccttgagt tttagaaaca gggacgctga ctgccgcagg ctctattacc tttagcgttc
    11521 cgctgatcct gtaaccagat atattctttt ctattttatc attattttca agaatttgta
    11581 cccctttttc ccttaatttt tcaacaaatt gccgatttgt ttctgaagct gtctgttcca
    11641 gctcttcttt tgtataacct ttctcgtaaa ccgtcatata ttcccctgct atctttccca
    11701 caaaaagcgg cagataaaaa tttgaaaaca gctttaactg gatcatatct gcggtgtaat
    11761 cccagttttc tttatttcca tttttcccca attcagggat caaacattca ggcataagaa
    11821 atgaaaagga aaaaggacct gcgatcaggc gcnnnnnnnn nnaccggcga taataacaaa
    11881 tacaactgtc tctttcggcc ccatgaccat cgttaataca ggagctgcga tcaggcgcag
    11941 accatagcgt ttccttcctg taggataacg cacgtcccgg gtcagaggca gttctttttc
    12001 ataagtttct actgtttctg ccagtatctc cccctgtgac tgcacataaa aagcagatat
    12061 ctcctgctca tcatccccga ttacaggaac acggccactg acaaggacct gtccctgctg
    12121 gatctcttct ccttttttca ctttaggaat tccctggcgc accatgatat aggtgatcgt
    12181 gccagttaca tctgaaacca gatcacaggg atcttccttc accgttgttt tagcctgtat
    12241 gatcttattt tccttgatct ggatcaccag cctggtgccg gatactctgg ccgataccca
    12301 ggtgatctga ggaaacgctt ccctcatccc agcttccagc tcctcacagt ttatcttttt
    12361 ctttttcatc ccatagcgca catcctgtgt atccagatag cgcaccaacg tatctgcagt
    12421 ataatggtga ttgccctgaa aatggatatc ccatatgaac aaagacataa tatagagcaa
    12481 aaggaaaaaa ctggaaagac ctgctgccgc ataccatcgt ttccggtttc tctgaagaaa
    12541 aaacggcagt ccccggcgtt cgcagatacg caggcggaca cccttacctc tggcgcagcc
    12601 ccttaaccgg taaaaatcct ttaaaaaaag ggaacattgg tactctccct ttttcaagca
    12661 taccagttcc cagaaaaata tcccgtttat cctgcaccgt tccagaaaat attccgggct
    12721 gatgcttttg atctgtattc ttacataacc ttttaaaagg cgaaaaaaat accggatcat
    12781 ccctccaccc tcctttctga taacagatca tcaaactcta ccgatcgtat ctgtcccttg
    12841 atctttaaag catcttccgt ataagtctct atctccagat gaagcccttt aaacactacc
    12901 atacagcctg ctgcctttat tttcacatac gtatctgtgt agataagaag gctcctgaaa
    12961 ttttccgcta ctacttcata aggacctgta aaatggagca gaacatcccc cagtattaca
    13021 tctcttggaa gaccaagagc ttccactgct gcccgttttc tgtcctctct cataccgttt
    13081 ctcccggagc cttttccttt tattatatga catccccggc cctgaaatgc cagtttggct
    13141 gtttacggct ttctgcaccc attttttctt ttaagaatac actaataggg aaagaatgat
    13201 tgtgaggttc ttatgactaa ttctaagtta aatcctgctt ctgaagccat ttccatggta
    13261 aaagcagctc agacaaatga agaagatgtt cctgtaactc ctctgccgga ccctggagaa
    13321 ggcggacctg tatatgatgg ttccatgaat aatgatccca tggataatgg ctccggcgga
    13381 aacggctcgt tcggcgatga tctgagttct gttcctgtta tccccctgcc aaatccagga
    13441 gagggcggac ctgtatacaa tggtcctaat tacaaccgtc catctggcgg tatgggacag
    13501 ccggggaatt cccagatacc ggtataccgt cctgtcttcc cgtctaatgg ctccattacc
    13561 gttctcccag gtatcagttt cccctgttac aactgcacta cctcctcaag ccttggccgg
    13621 gtacgcattt taaatgcttc ttccggttat ccggcattta acgtatcttt aggaaactgg
    13681 cgcattggag agcagttgga taacggggat ctcaccagct atgtacaggc agcctccggc
    13741 ttccagacgg ttacagtatc cggcactaac ggttatatct atatccagaa aatgatcacc
    13801 atccgtgccg gttctgcctc taccgttgcg atcatcaata ccagcaccgg gctggatctg
    13861 atggaaatat ccgacctttc ctgcaacgga ccatccggca ctgcctgcat ccgtgcctgc
    13921 aacctttccc cggaccttgg gccttttgat gtagctttag aaaaccgcgg caattcctac
    13981 cgcaccttca ccaacgtccg cttccaggaa gtaaccccgt tctccagttt cgcatccggc
    14041 tggtattcca tctacgtatt ccgcaccaat gcatttaaca acaataatgc cattgcagca
    14101 gcttcagcag cgttaaagac cggcggatct tatacattat atatgtttaa cgggccgtca
    14161 agtacggatg ggcttaggac gttgatattg tcgaattaat atctttttat ctgcataaca
    14221 atataagaac cggaagagct gtgactccca ttcacagctc ttccggtctt tcatactttg
    14281 tattcactta ctttctatcc ctttccagat ttttaatgct cagatctctt ccaaaaccaa
    14341 catcggttcc aaaccgttta aacagatcat atgccagact gtctatggtg gaaatatgca
    14401 tctgggactg ctgctctaat caggcaagat aacgttgatt tttggtcaaa gcatacttct
    14461 tcaacaacat tttctgcagc ctgtcattca tctggttctt tctttgcatt aaattttctt
    14521 taaaatataa aactgagtat ttcggtccca taaacattcc ttcccccgca cagtcgttcc
    14581 aaaccctttt gaaaaatcaa gttcaccctg ccattccagt gcagcagcta aagtgatccc
    14641 atcgcctgcc tgtttttcaa ccaacaattt cccacctgct gcgcaggccg accgttcaca
    14701 tacatttcct accccgatct gtttttttac aaaggcagac tctttaaaat cgcctggaac
    14761 ctgatttaac tgctctggcg tatagaaacg ctgctcacag ggttgatgta aacattctcc
    14821 gtcaaatagc tgttttaagt ctaataccgc aggttcatcc tgttttacat ctacagaagc
    14881 aatagctttc acagcggccg gatcgatccc atgttctttt aataaggcaa aaactttttc
    14941 tttcataaat tccggatctg ttcccctgcg gcacccaaca ccaaggacaa ccgccctggg
    15001 aacaagacgc aaataagaag cttttttttc cggaaaagaa ggatccttta ctgtgataca
    15061 aatattttct tcacaccatc tatccatcac aaagccgtct ggcaattttt ttattggaag
    15121 atcactgaaa acaccgatct gctttccttc taatactgct gctgatatgt tcttcgcttc
    15181 tttccggtct gtgatcgtca tatgataggc agatgcaaac agatctaccg caaacttccc
    15241 atttacatcc gttgctgtcg tgatcacagg cactgcctcc agccagtctg caatgtgcct
    15301 tgcaagttca ttggccccgc ctacatgccc tgatagcaag gggatcacaa actgcgctcc
    15361 ttcatccact gtaactaccg gcggatcagt catcttatcc ctgatccatg gcgcaatagc
    15421 ccgcactgca atacctgtgg cgcttacaaa gatcattgca cgcttttctg caaacatctt
    15481 gcctgtccat tgggataaag actcttcttc ccgttctttc agtccttctt ttttccactc
    15541 ttcccttaag aaacgcttgg gaataatcgc ttcacattct tcgcccgttt cttttaattt
    15601 atgaaaaaga cgcctgcaaa cccaggcgcc tcgttctgta aaacagatca ctgcatattt
    15661 catcctgcta cctgtacctt cttggatcaa atccggatca gaacatatag tttcattctg
    15721 atcaccagct tttcctacat tctggtatca gtatactgga ttttcagctt tctttcaatg
    15781 cttcctcttt taattgccgt ttccggccgg tgatcatccc tccaatgcgg ccgctttcct
    15841 tggaagtaag gcttttccag cctttctgca atactttttc atctaatccc agttcctggg
    15901 cgatctcaaa ttttaactgt tcatccggtg tcatattatg gacatcaaat tcttttttct
    15961 tttttgactt tcccataggt ctatactcct taatacgtga tgtattttca ttggtctgtt
    16021 ataagaagta tgcccttttt acaggaaatc attcaggaaa cactgagacg gcacctgctg
    16081 tcggctgttt ttccaaaatg caaaacaaaa agagcagccc cgcaaggaac tgccctttca
    16141 tcatttacat tcagttatta ctttgcgtaa gaatcgatat ctacgtcagc aacatcttcg
    16201 ttctgaggag ctgcctgaag agcctttacg cttaagctga tcttgtgatc atcttcgttg
    16261 aaatctacaa ccttagcttc gatttcctgg ccgatcttta atacatcagc tggtttctcg
    16321 atgtgctctc tggagatctg ggatacatga agcagtgcat caacgcctgg ctctaactct
    16381 acgaaagcgc caaagtcagt catacgagca acacgaccgg ttactacgct gcctgctgca
    16441 tacttctcag ctgcatgtgc ccatggatta gcctctggga actttaagct taatgcaatc
    16501 ttctcgccct ggatatcttt gatcagaacg gttaccttat cgccggactt gaataccttc
    16561 ttaggatttt caacacggcc ccagctcatc tcagagatgt gaaggagacc atcagctccg
    16621 cctaaatcga tgaatgcgcc gaagtctgtt acgttcttaa cagttccttc taccttatca
    16681 ccaacctgga tcttggagaa cagttcttct ttcagtttag ccttttcagt aactaacaac
    16741 tgctttctgt cgccaatgat tcttcttctc ttaggattga actcagtgat tacgaactgg
    16801 atttcctgtc ctgcgtactt ggaaagatcc ttctcataag tatcagatac aaggcttgct
    16861 gggataaata ctctggagcc ttctacttca acgcttaaac cgccgtctaa tacctgagct
    16921 actttcgcag tcagaacttc ctgattttca aatgcttctt ccagacgctt gttgcctctg
    16981 tcagctgcca tacgcttgta agataaggat accatgccaa caccatcgtt cagcttgata
    17041 accttagctt ccatttcttc accaacctgt accttggtag ttaaatctac actattgtca
    17101 ttgctgtact cgtcacgggt gataacaccc tcagacttgt tgcccgcaat gctcagaata
    17161 atctcatctt tattaacttc gatgacctga ccggtaacta tctctcctgt acgtattggt
    17221 ttggtggatt cttcctcttc ctttaataac tctgcaaaac tctgctctgc cattctggta
    17281 tgaacctcct caataatctt ttttggggtg gatgccccag ctgtaatacc tacgctgcgc
    17341 acagaagata cactgtcagg atctaaatcg ccgagtgtct gaatgtaaaa tgtattttta
    17401 cattccttga gacaaatctc gtaaagcttc tgggtgttgg aagaatgtct gccaccaatg
    17461 acgatcatag cctccaccga agatgctatc cgggctgcct ccacttgtct ttcctgtgtt
    17521 gcattgcaaa tcgtatttaa aacactacta tcataaccct tttttgaaat tttttcaact
    17581 aattcttgaa atttgttgta attaaatgtc gtctgggata cgatacatag ctttttgccc
    17641 tctggaaggt tataatcttc tgcatcagcc tcattttcca caacaacagt gtcaccattg
    17701 ccccagcccc ggataccttc cacctccgga tgcaccgggc ttccaatgat gataacagcc
    17761 tctccggccc gcgaatgttc atctacgatc ttatggatct tttttacaaa agggcaggtg
    17821 gcatctacca catttacccc acgggaattt aagatatcgt aaacatgacg gctgactccg
    17881 tgagaacgga tgatcacggt tccctgacct tccggcaggt tttcaaggtc ttcttccgta
    17941 tctaatacct ggacgtcctt ttcttccaga tcacggacta cttcctcatt gtggatgatg
    18001 ggaccatagg tatagatggg accctgggct tttccctctt ttacagcctg gatctgctca
    18061 tagacccggg ctactgcccg ttctactc
//
LOCUS       DBMDUA010000038        17870 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1861_length_17870_cov_3.846924, whole genome shotgun sequence.
ACCESSION   DBMDUA010000038 DBMDUA010000000
VERSION     DBMDUA010000038.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 17870)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17870)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17870
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1861_length_17870_cov_3.846924"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="ACZPCL_04785"
     CDS             complement(<1..1422)
                     /locus_tag="ACZPCL_04785"
                     /inference="COORDINATES: protein motif:HMM:NF024627.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR1161826.1"
                     /translation="MKNIHIRKQTGILLCILFALALSACTSKNITEPVEPAKQTFGAA
                     PIQKSEEAEAVMESQSEEIAEDNRFCGQWILAGYEYGERENAGDTPIRTPYYLAGDEG
                     EESKIHIYSEGDQLYADYYHCQNEGTTTINGMAFEEETTTTEDQPAARLKNRMDESEI
                     RRTVTLLEDNVLQYREQYYGMTSDYTYNGIYMREGSKELEQRSEYQYLDVVTVSTVEE
                     LVEAIQDRTKVILKGGEYNFSSLDDTEIQNPKLDAISYYDEPAEYTIKDLHNFCMEAA
                     NGEKVVVSTASPYGKVLAFEGCDNITVRGLTCGHEVEPGYCTGSVIYLSETNNVMIDD
                     CHLYGSGTYGVEAHNSRALHVEGTELFDCTYGLLELDNVTAARFQECTFRDSKQYNMF
                     SFQDCYNILLQDCLISGNHSDAGYFPFISAPNSYKVLFKNCEFKENTYSDFLAETDSL
                     SMERIVFENCLMDDGETVNTVDEH"
     gene            complement(1439..2413)
                     /locus_tag="ACZPCL_04790"
     CDS             complement(1439..2413)
                     /locus_tag="ACZPCL_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161827.1"
                     /translation="MIKIKKKGWIIFMTFSSWATLLAGCKKWPGDQSPVEITTEAPVA
                     ITDIQEISFQESGSMIPLVSYTTMTRENEGIRVQLELQYQYEYEKMMDASLMDQVQKI
                     LETYDVGKWNEFRGNDSMVLDGSSFSFHVTFTDGTSISASGNNAFPKNQRKVFSELNS
                     LIAPVVEQWYQEQYPKMIEDNRINGFSFYVCSKDQAQKFEWRSEKRNDDPQADYLYAD
                     IVNIDQFKPIGEVEYFFYGKVPKLPYEELQEIVERFHLAEWNGETYPASSEQEEKSFR
                     LLIEYQSGESIMASGDSLPDGYEAAEDTIISLLWDYIQENREQFVVWK"
     gene            complement(2438..2959)
                     /locus_tag="ACZPCL_04795"
     CDS             complement(2438..2959)
                     /locus_tag="ACZPCL_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161828.1"
                     /translation="MNNPYVTKKTTRPRPWQGAILGICGFFTIVIGSMVPDYVTKKGA
                     IDNGVLFALLLGSVTPILLVIREIWRCYKAKRIGNWFAYYRETSVTFDKLSKEISKSA
                     VKLIDELLKMGYLQNLHIDYEKRFVELTADQRLVKANVYITVTCPCCGGKNMVIQGKA
                     SKCIYCDQPLPTR"
     gene            complement(3332..4870)
                     /locus_tag="ACZPCL_04800"
     CDS             complement(3332..4870)
                     /locus_tag="ACZPCL_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024627.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR1161829.1"
                     /translation="MRKWNTFGLFVLLLTVLTACNQAQNTEQATKNAPVTAKAETDAE
                     EVEEVIKEAVPEAATDALIVSTTAELISSITPDAHIILKSGTYNFSVLTEAEIAGAGA
                     YVDPDLLKQGEFFVYNAPGLILEAEKSGSVRLVTENGYADVMTLSYCDGAVLKGLVLG
                     HEVEKGECDANVLKLLTSQSVTVENCSLFGCGTYGIYGEDAAVLTVIGTEIYECTNGI
                     LNLSETSHTVFEHCNFHDNDGMFFLWGDTQIQIRNTEISQNQGSLLQAYNSQLFDADS
                     IHITFQNCTFRGNRDMGIPKDWSCATFEACDFSSGSTPVLAGMTYEDLVRRYRDLAMD
                     PDAFQDADGAGEQNFLMIAGEMAADLGEDPADIMGYAIQDLNGDGVPELAIGFTPEYG
                     AYLSSLFTLAEGTPRLVFGEAGDGYTYLQDGSFFYNGCRSASENGKGIYQFTDDGTAL
                     ICREFYFLRILDGDESDAAVYYNSTGSWEIGDSRKTNMTVEEFWAWEPEYMYLPMTPF
                     SAAD"
     gene            complement(5218..6897)
                     /locus_tag="ACZPCL_04805"
     CDS             complement(5218..6897)
                     /locus_tag="ACZPCL_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012422.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1161830.1"
                     /translation="MFRRKIKDIQEHAKNRGISMERRLFLYWLIMVLAVMTAVFVVLI
                     VAGVFSNDAGKLGGTLSVQQKNTTMTFNKLIDTLNAQNLALSEQITREVSDVLETEER
                     TFKDLDNNPELITRIEERMCFYLQTIVRSRSCSGAYFILDATTNTEAPGADRSRMGLY
                     LRSGNVGTDGMANQYITFFRGVADVARKENIQMHNRWNLEFDIDNVPGYEILMDFDGR
                     RILDSCYLSNRVTLSGTWEQVVLLSIPVVLEGKVRGVCGVELSELFFNMVFPSVESPY
                     GNMVTVVAPIADKRLLLDGAISGKTDGTYFTTDGTLLIKNGEHYKTYSDGSRTYFGNH
                     IVIPKAVDGRSDLTLVTLVPEISYQSSVAKNRTFWLCGGLGFLVLMLLAAMFLTRRFM
                     LPIQKQIRALKDQKEPQEESNSGIIEIDELLAYMHARQAKPDEESKMPPGMEELFFDF
                     ARRVETLTPMERTVLQYYINGFSVNEIAEKEFISLSTVRKHNSNINRKLGVSTREELL
                     LYIDMFRRCDLMEKIAYCTGPEKKRTERTDEQQAAKTEKSPEKLKQEEKGS"
     gene            complement(6934..8307)
                     /locus_tag="ACZPCL_04810"
     CDS             complement(6934..8307)
                     /locus_tag="ACZPCL_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR1161831.1"
                     /translation="MKKDENRQKRTIKRTARVLAVLTGACLVMLLGGCGKKEKEPVTV
                     TLWHVYGGQTESPLNDLIDEFNETVGKDKNIRVEVGSVTNTNIIHEGVLAAANKEPGA
                     SELPDMFVSYPKTVTAMPDPDVLVNYKDYFTDEELSAFIPAFVEEGTIAGKLAILPVA
                     KSTEILFYNKTAFDRFAEETGADPSEFETWDGLYKLAEVYEDWCGGTKPFFVHDYHFN
                     YFQVGVESLGESFFDEKGVCFGPAFRRVWEPYARAALKGGVWLQGGYATEPLRTGDSI
                     VSVASSASVLYYSTTVTYADNTTEEVEIKALPCPVFEDGKKLVMQRGAGICTVKSTPE
                     REEACMVFLKWLTDPQKNVEFVTQLGYMPVKQESFDVYLPKALEGIQDPMYRSLYEAF
                     LKTQEEYEFYTAPQRPDYLKLETDFEHDIREDLDMFRKRYLETIAEDPEPPVWKALDQ
                     FEMTFSQ"
     gene            complement(8691..10091)
                     /gene="gtfA"
                     /locus_tag="ACZPCL_04815"
     CDS             complement(8691..10091)
                     /gene="gtfA"
                     /locus_tag="ACZPCL_04815"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HIR1161832.1"
                     /translation="MLNKIMLITYPDSMGHNLKDLRHILNRYFKDAVKMVHILPFFPS
                     SGDRGFAPLGYEKVSEEFGDWEDIRMLSEEYPLMFDYMINHISAHSPYYQDFLENKDQ
                     SPYRGLFIRYKDFWKNGIPTQAETDAIYKRKPRAPYVMAEFGNQTQEAVWCTFGEEQI
                     DLNMETEEGKRFLEENLRWLARHGASLIRLDAFAYAVKRPGTSCFFVEPDIWELLERC
                     AKIAAEEGAQILPEIHEHFSIQQKLACRDYYVYDFALPMLLLHAIYFKNSEYLKHWFE
                     ICPRKQFTTLDTHDGIGVVDVRGLLPDEEIEAAKEHLFEYGANVKRVYNTEKYNNLDI
                     YQINCTYYSALGDDDNAYLLARAIQFFAPGTPQVYYVGLLAGKNDISLLEETKEGRNI
                     NRHYYTEEEIGREVERPVVKQLLKLMELRNTHPAFDGEFYMLPCEKEQLILGWKNGGS
                     RIELHGDLGKKSFEII"
     gene            complement(10125..11630)
                     /locus_tag="ACZPCL_04820"
     CDS             complement(10125..11630)
                     /locus_tag="ACZPCL_04820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR1161833.1"
                     /translation="MTGKKFERTTPEAVGISSKSLERLIENLEQVTEMHGIVVMRHEK
                     ICAEGWWAPYAPGIRHSLHSLSKTYSATAVGIAVTEGKVSLDEKILSIFSEKAPENPD
                     EYLKQLTVKDILCMGSGMERLPEVTENWLQDYLAVPIKHRPGTAFQYNSVGSTLLCAI
                     VERKTGIPAEEYLKSRLFDKIGIDSDNFLFERMPDETVFGGGGFYATTEDNLRLMKLY
                     KDGGVWDGERILSEEYVKMAISKQIDTATEKNGNPEATDNFLGYGFQIWVCQPENVYR
                     ADGAMGQFSIVFPDQDMIISVNETASNAHWAQNTLDEIWKFAREVWEETLPEDEAAST
                     HLKEKLSHLSLEAPCRQCSRNREKESEINGKCYFVSEGRMGFETEIQNSHAGLKPLEG
                     ITEFTMRFVPGTCEMKFCQQGKEHEVLIATDGSWRWNRLKLDGRVNSELCLSGYWEDK
                     NCFVVHARWIETCYENELKFCFEKDQVHMSRMNKVGRYMTSQPLTATAQKA"
     gene            complement(11678..12574)
                     /locus_tag="ACZPCL_04825"
     CDS             complement(11678..12574)
                     /locus_tag="ACZPCL_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1161834.1"
                     /translation="MKTRLGGERSFRRFALITLGIMTILSLLPVILIVIASFTDENSL
                     IANGYSYFPAKWSLDAYFYMIKQAKLITRAYGISFFVTITGTLLSVLFTTTLAYPMSR
                     KDFKFSKILTFLVFFTMLFNGGVVPSYMMWSQFFHLKNTIWALILPNYLITAFNIMLV
                     KNYYQNSIPDALIEAAEIDGASELTIFWKIVFPLSTPVIATISLFTALKYWNDWVNAL
                     YYITKPQLYGIQNLLLRIMDNIQFLKSGGAGEIAGAAMVDLPGTSVRMAMAVVGILPI
                     ILIYPFVQKYFIKGVVVGAVKG"
     gene            complement(12592..13515)
                     /locus_tag="ACZPCL_04830"
     CDS             complement(12592..13515)
                     /locus_tag="ACZPCL_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161835.1"
                     /translation="MTGRKRKGRGTKNVPLLLIALPGLAYLLINNYIPMFGIFLAFKS
                     YDFSKGVFGSPWCGFDNFKFLFKTKDALIMTRNTILYNFAFIVIGTALGIFVAILLTE
                     IVKSVWAKFYQSALLLPYLLSWVVISYMVFAFLNSDSGFINNAVLKVLGMAPVSWYTK
                     SNIWPFILILVFLWQTVGHTSIIYMASIAGIDKGIYEAAKIDGAGKIKQIFYITLPLL
                     RPTITIMVLMAIGKIFFSDFGLFYQVPMNSGALYGVTQTIDTFVYRGLMELNDVGMSS
                     AAGLFQSCVGFVLVITANALVRKMNPENALF"
     gene            complement(13526..15079)
                     /locus_tag="ACZPCL_04835"
     CDS             complement(13526..15079)
                     /locus_tag="ACZPCL_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013697.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1161836.1"
                     /translation="MKKSMKNRVAGAAAVLLAGSMAFSMTGCGSSSDNTAGQQTATDQ
                     KTEGNEEYTKIVYAFKSFNNIPEDLSDVNEAISEITREKIGVEVELMPIASSAYGQQV
                     SLAMQGGEQLDLFHTGSGLELSTCLANHQLYDITDIVKEHAAGAIDAVGEDFMKTEVV
                     DGKVYGIPADKGVALAPTLLYREDIMEEIGVDPADIKNINDLTDVYAKVKEAYPDMIP
                     LVPVNPGDSGMINTIYGFDYLGDRYLSPTGILNGDDMQVENFYETDGFKDILTLARDW
                     YNKGYIMGDAATTTSTSIELLSAGKGFSYISNYGGNDAATQISTQTGQKIGALRLSQP
                     YLGTSDVNRVSWAVASTCKNPEKALEFLNLTYTDKDVINLIIYGIEGKDYVKVSDNVV
                     KYPDGQDASTVPYTAQLSCGVCGSQFNQYILEGASEDTQKVMKEDNQNAGRSKAFGFT
                     FDSSTVKTQYTAVNNVINQYLPGLRCGSLDPETELPKFQQSLKEAGIDEIIAEKQKQL
                     DAWAAENAN"
     gene            complement(15174..16766)
                     /locus_tag="ACZPCL_04840"
     CDS             complement(15174..16766)
                     /locus_tag="ACZPCL_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1161837.1"
                     /translation="MNVLIVDDDMATVDVICKSVNWSALHIDKVMTAYNIEKAKEMLA
                     DIPADIVVCDIEMPMGNGTELLKWVRENDMSSEFLFLTCHTEFQYASVAIRYQAAGYL
                     VKPFRVEQVEAELLRLVQRIMEKRKIEEESHYGKWMRNNQEQLMNNFWRALIERELRA
                     ESEFIDREIKKRRLPVFADQPVRLIGIRVDREEQAKEALGENLLEFALEKMVSEIVDE
                     SVENTSVIKKIVNEALFFLVIDWKRTLEEELMERCHILIKTGQDYVGCCFTCCIGNPC
                     SMEQLPDKADEVINLLLRNVLESDQVFHEGEAAVTSGVNTRLLDLKQMENDLRSKDRM
                     KILNTVKTVLGRLSREKSVTVETLLVIQQEILQLIYTNLYQNGIQAAELFGDEVSQKI
                     QRQSVRSVFDMIKWVNYTVSRTLEYEDEINRPLSLADKIESYVKEHYKEDISRDTIAD
                     VFFLTPEYLAKVYKRQTGHNLKDYIIGYRIEMAKQLLADDIKSISDISAEVGFDNFSY
                     FSTVFKKYTGITPGEYRRSKKS"
     gene            complement(16744..>17870)
                     /locus_tag="ACZPCL_04845"
     CDS             complement(16744..>17870)
                     /locus_tag="ACZPCL_04845"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1161838.1"
                     /translation="VNGLCVENLEEGNSMYLGETLEIEDSWKNDHKKRMISLKSDRAG
                     LYITGMIGSNHFLGKFSTNSILILLIMVLAIGCDMILLHYIHREIHRPLDHLMQTIQE
                     IEDGDYKQRAEEDYHTKELQRLTQAFNRMMNVIVNLRIRAYDEKIQLMDTQMKYFQMQ
                     LKPHFFLNALTTIYSMSYQKRNEDIRTYIDALTKTVRYMFKGGLRLVLLKEETQHLEN
                     YFEMQQLRYPDCVFWYFDMEEAAMSWKIPQMLLHTFVENEYKYAVKIDSVLSILIQAK
                     VLLKEGEEMLYIRMEDDGVGFPEQVMQYMNFESEDPSREGHHVGLWNIKRTMEVLYQK
                     RELIHLSNLETGGCLIEIWIPKTTAVGEEQEIKNERIDCG"
BASE COUNT         4754 a         4551 c         3142 g         5423 t
ORIGIN      
        1 atgttcatca actgtattta cggtttcccc atcatccatc agacaatttt caaatacaat
       61 ccgttccata ctcaggctgt ctgtctcagc taaaaaatca ctgtaggtat tctccttaaa
      121 ctcacaattc ttaaacagga ctttatatga attcggagcc gatataaatg gaaaataccc
      181 tgcatcagaa tgatttccgg agatcagaca gtcctgaaga agtatattat agcaatcctg
      241 gaaggaaaac atattatatt gcttgctgtc ccggaatgta cattcctgaa atcttgctgc
      301 agttacattg tccagttcaa gaagtccgta agtacaatca aacagctctg ttccctccac
      361 atgcaaagct cttgagttat gtgcctccac accgtaagtt ccgcttccat acaaatgaca
      421 gtcatcgatc atgacattgt ttgtctcgct caaatagatc acacttccgg tacaatatcc
      481 cggctctacc tcatggccac aggtcagtcc acggaccgta atattgtcgc agccttcaaa
      541 agccagtact tttccatagg gtgatgccgt gctgaccacg accttttcac catttgctgc
      601 ttccatgcag aagttatgga gatctttgat cgtatattcc gccggttcat cataatacga
      661 aatcgcatcc aatttaggat tctggatttc tgtatcatca agactggaaa aattatattc
      721 tccccctttt agaatgacct tcgtcctgtc ctggatcgct tccaccagtt cctccaccgt
      781 tgatacagta acaacatcca gatactgata ctcactacgt tgttccagtt ccttcgatcc
      841 ttcccgcata tagattccgt tatatgtata atccgatgtc atgccataat actgttcccg
      901 gtattgcagc acattgtcct ccaaaagagt cactgtacgg cgtatttccg attcatccat
      961 tctgtttttt aagcgggcag ccggctgatc ctctgtggtc gttgtttctt cctcaaatgc
     1021 cataccattg atcgttgttg ttccttcatt ttggcaatga taataatctg catatagctg
     1081 gtcaccttct gaataaatgt ggatcttaga ttcctcgcct tcatcccctg ctaaataata
     1141 tggagttcta atcggcgtat cccctgcatt ctcacgttcc ccatattcat aaccagcaag
     1201 tatccattgt ccgcaaaacc ggttgtcttc tgcgatttct tctgactggg attccatcac
     1261 agcttccgct tcttctgact tctgtatcgg tgctgcacca aaggtctgct ttgccggttc
     1321 taccggttct gttatattct ttgaagtaca tgcactcaat gccagcgcaa aaagtatgca
     1381 caaaaggatt cctgtctgtt ttctgatatg tatatttttc atagcactca cctcttaatt
     1441 atttccaaac tacaaattgt tcacgattct cctgaatata atcccagaga agtgagatga
     1501 tcgtatcttc cgccgcctca tatccatcgg gaagagaatc tccggatgcc atgatcgatt
     1561 cgcctgactg gtattcaatg agcagtctaa agcttttctc ctcttgttct gatgaagcag
     1621 gataagtttc cccattccat tccgccaggt ggaacctttc tacgatctcc tgtaattctt
     1681 catacggcag cttcgggacc tttccgtaaa agaaatactc cacttcaccg atcggtttaa
     1741 actggtctat attaacaata tccgcataca ggtaatcggc ttggggatca tcgtttcttt
     1801 tttcagatct ccactcaaat ttctgcgcct gatcctttga acagacataa aacgaaaaac
     1861 cgttgatcct gttatcctcg atcatcttcg gatactgctc ctgataccac tgctcaacga
     1921 cgggagcgat cagggaattt aactcggaaa agacctttct ctgattcttt ggaaatgcat
     1981 tatttccaga tgcagaaatg gatgttccgt ccgtaaacgt aacgtgaaat gaaaaactgc
     2041 tgccatccag aaccatactg tcatttcccc tgaattcatt ccattttccc acatcatagg
     2101 tttcaaggat tttttgcacc tgatccatca gagaagcatc catcatcttc tcatattcgt
     2161 actgatattg aagctccagc tgaactctga ttccttcatt ctcccgggtc atggttgtgt
     2221 aagaaactaa agggatcatg cttccgcttt cctggaatga aatctcctgt atatcggtga
     2281 tcgccaccgg tgcttctgtt gtgatttcta cagggctttg atcacctggc cactttttgc
     2341 acccggcaag taaggttgcc catgatgaaa acgtcataaa tatgatccac cctttctttt
     2401 tgatcttgat cattaccacc tcacattcac gcaaaactta ccgcgtcggc aaaggctgat
     2461 cacaatagat gcatttgctg gcttttccct ggatcaccat attttttcct ccgcagcatg
     2521 gacatgtaac ggtgatataa acatttgctt tcacaagacg ctggtctgct gtcagctcca
     2581 caaaccgttt ttcatagtcg atatggagat tctgcaggta tcccattttt aacagctcat
     2641 cgatcagctt aactgcactt ttactgatct ccttcgataa tttgtcaaat gtaacactgg
     2701 tctcccggta atacgcaaac cagttgccaa tccgttttgc cttataacac cgccagattt
     2761 ctcttataac cagcaggatc ggcgtaacac tgccaagcag caatgcaaat agcactccgt
     2821 tatcaatggc acctttttta gtaacataat ccggaaccat cgaaccaatg acaattgtaa
     2881 aaaaaccaca tatcccaagg atcgctccct gccacggtct cggtcttgtt gttttttttg
     2941 taacatacgg attattcatt ctcatttccc cctctttgtt tcatccgatc tatttctttc
     3001 tttatactgt attatgtaca gaaaatatat ggtcgcagtg agtattttgg cgtttttaca
     3061 caatatactc attgtgagta tacaagatat agtgttttat ttttcaattc tatctatata
     3121 ttgtttttat ctgcaaatac attctgtttt attgcagtac gatccagacc agatgtattt
     3181 ttatttcaca ggacacaatt tcctatgaaa agcaaaaaaa gccccgcagt aataaactgc
     3241 agaacttttc ttttatcgtc ttctgttgtt gtaaaggaat attgtaaaag actttcttat
     3301 gatctgcagc acagatcact gtgcttatcc cttagtctgc tgcactaaaa ggagtcatcg
     3361 gcaaatacat atattccggc tcccacgccc agaattcctc cactgtcata ttggttttcc
     3421 gggaatcccc gatttcccag ctcccagtgg aattataata gactgctgca tccgattcat
     3481 ctccatccag gatccgtaga aaatagaatt ccctgcagat cagcgctgta ccatcatctg
     3541 taaactgata gatccccttt ccattttccg atgcgctgcg gcagccgtta taaaagaagc
     3601 tgccatcctg cagatatgta taaccatctc ctgcttcccc aaagaccagc ctgggcgttc
     3661 cctctgccag tgtaaacaga gaagacagat atgctccgta ttctggtgta aatccgatcg
     3721 ccagctccgg cacaccatca ccattaagat cctgaattgc atatcccata atatccgccg
     3781 gatcttctcc caggtctgcc gccatctctc cggcgatcat gaggaaattc tgctcccctg
     3841 ctccatcagc atcctgaaat gcatccggat ccatcgccag atcccggtat ctcctgacca
     3901 gatcctcata cgtcattcct gctaagaccg gtgttgaccc ggatgaaaaa tcacaagctt
     3961 caaaggtagc gcaggaccag tcctttggaa ttcccatatc acggttgccc cggaaggtac
     4021 agttctggaa agttatatga atagaatctg cgtcaaaaag ctgactgtta tacgcctgca
     4081 gcagactacc ctgattttgg gagatttctg tattccggat ctggatctgg gtgtctcccc
     4141 ataagaagaa catcccatca ttgtcatgga aattgcagtg ttcaaaaact gtgtgtgaag
     4201 tctcagacag attcaaaata ccgttggtac attcataaat ctctgttcct ataacagtaa
     4261 gtaccgctgc atcctctcca tagattccat atgtgccgca gccaaatagg ctgcaattct
     4321 ccacagtcac actctgggaa gtaagaagct tcaggacatt ggcatcacat tccccttttt
     4381 caacctcatg tcccagaacc agccccttca acactgcacc atcgcagtac gaaagagtca
     4441 tcacatccgc atatccattc tccgtaacca gccgcacaga tccgctcttt tctgcctcca
     4501 gaatcaatcc aggagcattg taaacaaaaa actcgccctg ctttagcaga tccggatcca
     4561 cataagcgcc agcccctgcg atctccgcct ccgtcaatac tgaaaaattg taggtaccgg
     4621 atttcagaat aatgtgggca tccggcgtta tggacgatat caattctgca gtggtagaaa
     4681 caataagcgc atctgtggct gcctctggaa ctgcttcttt aattacttct tccacttctt
     4741 ctgcatccgt ttcggccttc gctgtaaccg gagcattttt tgttgcctgt tccgtatttt
     4801 gtgcctgatt gcaggctgtc agcaccgtca acagcaggac aaacagccca aatgtgttcc
     4861 attttctcat ggcatcctcc ttatgtttct aatattctcc cggaatcttt ctgtacctga
     4921 ttttgtgccc gttttctttt cctctgcttt atttcttttc ctctgctttt ttgaaattcc
     4981 aacaatccat ttcgaaaatc ttctttataa atagtctaca tgatttcata aaaaaatata
     5041 tactcaccat aagtatcttc tgctgttata aacaaaatac tcacagcgag tatactatat
     5101 ataggcacaa aatcagtttg cacgtcaata tatagcattg ttctgatttt tataaaaatt
     5161 aaacgggaga acacttctga tcaaagcatc tcccgtttaa aatatgtttt ctgtttttta
     5221 tgatcctttt tcttcctgtt tcagcttttc cggggatttc tccgtctttg ctgcctgctg
     5281 ctcatctgtt ctctctgtcc tttttttctc cggaccagta caatatgcga tcttctccat
     5341 aagatcacac cgccggaaca tatcaatata aagaagcagc tcttctctgg ttgatactcc
     5401 aagctttcga ttgatattcg aattgtgctt tcttactgta ctgagactga taaactcctt
     5461 ctccgcaatt tcatttacac tgaaaccgtt aatataatac tggagtaccg tccgctccat
     5521 cggagtcagt gtttccaccc gtcttgcaaa atcaaagaac agctcttcca tgcccggagg
     5581 cattttactc tcctcgtcgg gcttcgcctg tctcgcatgc atataggcaa gaagctcatc
     5641 gatttcaata atacctgaat tagattcctc ctgtggttcc ttctggtctt tcagcgcacg
     5701 gatctgcttc tggatcggca gcataaaccg ccttgtaagg aacatggcag caagaagcat
     5761 caaaacaaga aaaccaagac ccccgcaaag ccagaatgtc ctgtttttcg ccacactgct
     5821 ctggtaactg atctccggta caagggtaac aagagtcaga tcacttcttc cgtccactgc
     5881 tttcgggatc acaatgtgat ttccaaagta tgtacggctg ccgtccgaat aagttttata
     5941 atgctcccca tttttgatca gaagcgttcc atccgttgta aaataggttc cgtctgtctt
     6001 tccggaaatc gcaccatcca gaagaagacg cttatcagcg atcggtgcaa caactgtaac
     6061 catattcccg taagggctct ccactgacgg gaataccata ttaaaaaaca gctccgaaag
     6121 ttctacgccg cagactccac gtacctttcc ctcaaggact accggaatac tcagaagaac
     6181 tacctgttcc catgttccgg aaagcgttac acggtttgaa agatagcagc tatccaaaat
     6241 acgtctcccg tcaaagtcca tcaggatctc atagccggga acattatcaa tatcaaattc
     6301 cagattccag cgattgtgca tctggatatt ctcttttctt gcaacatctg cgactccgcg
     6361 gaaaaacgtg atatactggt tcgccattcc gtccgtaccc acatttccgc tccgcagata
     6421 cagccccatt ctggagcggt ctgctcccgg tgcctctgta ttcgttgttg catcaaggat
     6481 aaagtacgca ccactgcagc ttcgtgagcg gacgatcgtc tgaagataga agcacatccg
     6541 ttcctcgatc ctggtgatca attccgggtt attatccaga tctttaaaag tccgttcctc
     6601 tgtttccaga acatctgata cctcccgcgt aatctgctcg gaaagtgcaa gattctgtgc
     6661 attcagtgta tcgatcagct tattaaacgt catggttgtg tttttctgct ggaccgaaag
     6721 cgttccacca agctttccgg catcattgct gaacactccg gcaacgatca acacaacaaa
     6781 tactgctgtc atgaccgcaa gcaccatgat cagccagtac agaaacaatc gccgttccat
     6841 actgatcccc cggtttttgg catgttcctg tatgtctttt atctttcttc gaaacatatt
     6901 caaaaaccgc cccccttcct gtatttagaa ccatcactgg ctaaacgtca tctcgaactg
     6961 gtcaagcgcc ttccataccg gcggttctgg atcttcggcg atggtttcta agtatctttt
     7021 ccggaacata tcaagatctt cccggatatc atgctcaaaa tcggtctcca gcttcaggta
     7081 atccggtctc tggggcgcag tatagaactc atattcttcc tgcgtcttta aaaatgcctc
     7141 atataaagac cggtacatcg gatcctggat cccttcaagt gcctttggaa ggtacacatc
     7201 aaaagactcc tgttttaccg gcatataacc aagctgggtc acaaattcta catttttctg
     7261 cggatccgtc agccatttca ggaagaccat acatgcttcc tcccgctccg gcgtagactt
     7321 tacagtacag atccccgcgc ctctctgcat aacaagcttc ttaccatcct caaaaaccgg
     7381 acacggaagc gctttgatct ccacctcctc cgttgtatta tccgcataag taaccgttgt
     7441 tgaataataa agaacactgg ccgaagacgc aacggaaacg attgaatccc cggtccgcag
     7501 cggctccgta gcatagccgc cctgaagcca tacgccgccc tttaatgctg ctctcgcata
     7561 aggttcccag acccggcgga atgcagggcc gaaacagact cccttttcat caaaaaacga
     7621 ctctcccaga gactctacac caacctggaa ataattaaaa tgatagtcat ggacaaaaaa
     7681 cggcttagtt cccccacacc agtcctcgta cacctctgca agcttgtaca gaccatccca
     7741 ggtctcaaac tccgaaggat ctgccccggt ctcctctgca aaacggtcaa aggcggtctt
     7801 attataaaac aggatctccg tggatttcgc aaccggaagg atcgcaagct ttccggcaat
     7861 cgtcccctcc tccacaaaag caggaataaa agccgaaagc tcctcatctg taaaataatc
     7921 cttataatta acaagaacat ccggatccgg cattgcagtc acagttttcg gataagaaac
     7981 gaacatatcc ggaagctccg atgctcctgg ttccttattg gccgccgcaa ggactccttc
     8041 gtggatgata tttgtgtttg tcacactgcc cacctctacg cggatatttt tatcttttcc
     8101 cacggtctca ttaaattcat cgatcagatc atttaaaggc gattccgtct gcccgccata
     8161 aacatgccac agtgtgaccg tcaccggctc cttctctttc ttcccacagc ctccaagaag
     8221 catcaccaga catgctccgg taagcactgc aagaaccctg gcagtccttt ttatcgtcct
     8281 tttttgtcta ttttcgtctt tcttcatact ccctcccatg tatgacgatg taaataaaaa
     8341 ctttctttat attctaacac aaaaacagac tttctcaaat attattgtgt tattttgaac
     8401 acttaacttt tctcctgtta agaaaacaac actggcgttt gcccatttat atattctttt
     8461 atgtattttt atgtgttctt tatatctgca cttacatcct tttccttttg tttatttctc
     8521 ctttaatctc ttccattttc tcttgcctat ttttttactt atctttcatt gacctaaact
     8581 ataacgggag tttttgataa gagtgtgaaa aaatgtgagg agtttaaaat ttcaaacacg
     8641 ttaaaaagga acaggactga ttcaaataaa tcagccctgc tcctttggtt ttaaataatt
     8701 tcaaaggatt tcttccccag atctccatgc agctcaatcc tgctgcctcc gtttttccac
     8761 ccaaggatca actgctcctt ctcacatgga agcatataaa actcaccatc aaatgcagga
     8821 tgcgtatttc tgagttccat aagtttcaac aattgcttta ctaccgggcg ttcaacttcc
     8881 cgtccaatct cttcttccgt ataataatga cgattgatat ttcgtccttc cttcgtctcc
     8941 tccaaaaggg aaatatcatt ttttccagcc agtagtccca cataataaac ctgaggcgta
     9001 cctggcgcaa agaactggat cgctctggct aaaagataag cattatcatc atccccaaga
     9061 gccgaataat aggtgcaatt aatctgataa atatccagat tattgtattt ctccgtattg
     9121 taaacacgct ttacattagc gccatactca aacaaatgct ctttcgcagc ttcaatctcc
     9181 tcgtctggca aaagcccccg gacatctact acgccaattc cgtcatgggt atccagcgtt
     9241 gtaaactgct ttctcggaca tatttcaaac cagtgcttca gatattcact gtttttaaaa
     9301 taaatggcat gtaaaagcag cattggcagc gcaaagtcat acacataata atcccggcat
     9361 gccagttttt gctggattga aaaatgttca tggatctccg gaaggatctg agcgccttcc
     9421 tctgcagcaa ttttcgcaca tctttcaaga agttcccaga tatccggttc cacaaaaaag
     9481 caggaagttc ccggtcgttt cacagcataa gcaaaagcgt ccaggcggat aagcgatgca
     9541 ccgtgtctgg ccagccagcg aagattttct tccaaaaagc gctttccttc ctctgtctcc
     9601 atgttaaggt cgatctgttc ctctccaaag gtacaccaga ctgcctcctg ggtctgattc
     9661 ccaaactctg ccataacata aggggctctg ggcttccgct tataaatagc atccgtttct
     9721 gcctgggttg gtattccatt tttccaaaaa tccttataac ggataaacaa tcctctgtac
     9781 ggcgactgat ctttgttctc taaaaaatcc tgataataag gactgtgtgc ggagatatga
     9841 ttgatcatat agtcaaacat caatggatat tcttcggaaa gcatacggat atcttcccag
     9901 tctccgaact cttctgatac cttttcatat cctaacgggg caaaaccacg gtccccactg
     9961 gaaggaaaaa acggcaggat atgcaccatt tttaccgcat ctttaaaata ccggttcaaa
    10021 atatgacgca ggtcctttaa attatgtccc atggaatcag gatatgtgat cagcatgatc
    10081 ttattcagca tttatgaaat cacctctttt tgtccttttt attttcaggc tttctgcgcc
    10141 gttgctgtaa gcggttgcga tgtcatataa cgccccactt tattcattct gctcatatga
    10201 acctgatctt tttcaaagca aaacttcagt tcattctcat aacaggtttc tatccatctt
    10261 gcatggacta caaaacagtt tttatcttcc caatatccac ttaaacacag ctcactattt
    10321 acccttccat ctagtttaag ccggttccat ctccatgatc catctgtagc aatcagaact
    10381 tcatgttctt ttccctgctg gcaaaacttc atttcgcagg ttccaggtac aaatctcatg
    10441 gtaaattctg ttattccctc aagtggtttc aatcctgcat gggaattctg gatctctgtt
    10501 tcaaagccca tacgcccttc tgacacaaaa tagcattttc catttatttc agactctttt
    10561 tctctgttgc gactgcactg ccgacaaggt gcttccaggg ataaatgaga cagcttctct
    10621 ttcagatgcg tagatgcagc ttcatcctcc ggcagtgttt cttcccagac ttccctggca
    10681 aacttccata tttcatccaa tgtattctgc gcccaatgag cattcgaagc tgtttcattt
    10741 acggaaatta tcatatcctg atctggaaat acaatagaaa actgacccat tgccccgtca
    10801 gcccggtata cattttccgg ctggcacacc cagatctgaa atccatagcc aaggaaattg
    10861 tctgtagcct ctggattgcc atttttttct gtggcagtat cgatctgctt tgatattgcc
    10921 attttcacat attcttcgga taagatacgc tcaccatccc agactccgcc gtctttataa
    10981 agcttcatca gccttaaatt atcctctgtt gtggcataaa atcccccgcc gccaaaaact
    11041 gtctcatccg gcatacgctc aaacaaaaaa ttatcactgt caataccgat cttatcaaac
    11101 agtctgcttt ttaaatattc ctctgcagga attcccgttt ttctctctac gatggcgcaa
    11161 agaagagtgg accccacact gttgtattga aacgcggttc ctggacgatg cttaatggga
    11221 actgccagat aatcctgcag ccagttctct gtcacctcag gaagcctttc cattccactt
    11281 cccatgcaga ggatgtcctt taccgtcagc tgcttcagat attcatctgg attctctggt
    11341 gccttctctg agaaaataga aagaatcttc tcatctaaag aaacttttcc ttccgttact
    11401 gcaattccta ctgcagtagc agaataggtt ttgctcaaag aatggagact gtgtcggatt
    11461 cccggcgcat aaggcgccca ccagccctct gcacatattt tttcatggcg catgaccaca
    11521 atgccatgca tctctgttac ctgctccaga ttttcaatca gacgttccag gctcttactg
    11581 gaaattccca ctgcttccgg agtagttctc tcaaatttct tccctgtcat catttttctc
    11641 ccaaactata aacattgcaa atagtcgcca atctttttta gccttttact gcgcctacaa
    11701 ctacaccttt gataaaatat ttctgtacaa atggataaat taaaatgatt ggcaaaattc
    11761 ccacaactgc cattgccata cgcacagagg tgcctggaag atctaccata gccgcgcctg
    11821 caatttcacc ggctcctcct gattttaaga actggatatt atccataatg cgaagcagca
    11881 ggttctgaat tccgtaaagt tgcggttttg taatgtaata cagagcattt acccagtcat
    11941 tccagtattt cagcgctgta aaaagactga tcgtagcgat cacgggcgta gataatggaa
    12001 agacgatctt ccagaagatc gtaagttccg aagctccgtc aatttctgca gcttcgatca
    12061 aggcatctgg aatactgttc tgataataat tcttaaccag cataatgtta aaagctgtga
    12121 tcagataatt tggcaggatc agcgcccaga tcgtgttctt caagtgaaaa aactgagacc
    12181 acatcatgta ggacggaaca acgccgccat taaacagcat ggtaaagaac accagaaatg
    12241 taagaatctt tgaaaattta aaatcctttc tggacatagg atatgccagt gtggtagtaa
    12301 aaagcacact taagagcgtt ccagtgatcg ttacaaaaaa ggagatccca taagcccttg
    12361 tgatcagttt tgcctgcttt atcatataaa aataagcatc cagactccat tttgcaggaa
    12421 aataagaata tccatttgcg atcagactgt tttcatctgt aaatgacgca ataacgatca
    12481 gaataaccgg aagcaatgat aaaatggtca tgatacctaa tgttatcaga gcaaaacggc
    12541 gaaagctgcg ttccccgcct aatctcgttt tcattttatt gcctccttcc attaaaacag
    12601 cgcattttca gggttcatct tacggaccag tgcattagca gtgataacca ggacaaatcc
    12661 aacacatgac tggaacaatc ctgctgctga tgacattccc acatcattta attccattaa
    12721 tcctctgtat acaaaggtat caatggtctg ggttacacca taaagagcgc ctgaattcat
    12781 aggtacctga taaaacagtc caaaatcaga aaagaatatc tttccgatcg ccataagaac
    12841 catgatcgtg atcgttggcc ttaagagtgg cagtgtgatg taaaagatct gcttgatctt
    12901 acctgccccg tcgatcttgg cagcttcata aataccttta tcaatacctg caatgctggc
    12961 catgtaaatg atcgacgtat ggcccactgt ctgccataaa aataccagaa tcaggataaa
    13021 cggccagata ttactttttg tataccagga taccggagcc attcccaaca ctttcaacac
    13081 agcattgttg ataaatccac tatctgaatt taaaaacgca aaaaccatgt aggagatcac
    13141 cacccaggaa agcaggtatg gtaaaagcag cgctgactga taaaactttg cccagacgct
    13201 ttttacgatc tctgtcagca ggatcgccac aaaaatacca agagctgtac caatcacaat
    13261 aaaagcgaaa ttatataaga tcgtatttct tgtcatgatc agcgcgtctt ttgtcttaaa
    13321 cagaaacttg aaattatcga acccgcacca ggggctccca aatactccct ttgaaaaatc
    13381 ataactttta aaagccagga agatcccgaa cattgggata taattgttta tcagaagata
    13441 agcaaggcct ggcaatgcga tcaatagcag ggggacgttt tttgtccccc gcccctttct
    13501 ttttcttcct gtcataatag ctcacctagt tcgcattttc tgcagcccat gcatccagct
    13561 gtttctgttt ttccgcaatg atctcatcaa ttccggcttc ttttaagctc tgctggaact
    13621 ttggaagttc tgtttcagga tcaaggcttc cgcagcgcag tcctggaaga tactgattga
    13681 tcacgttatt tactgctgta tactgtgttt tcacagttga actgtcaaag gtaaatccaa
    13741 atgcctttga tcttcccgca ttctgattat cctctttcat aactttctgt gtatcttcgc
    13801 tggcaccttc caggatatac tggttaaact ggcttccgca tacgccgcag ctaagctggg
    13861 ctgtatatgg aacagtgctt gcgtcctgac cgtccgggta tttcactaca ttgtcgctga
    13921 cctttacata atcttttcct tcaattccgt aaataatcag gttgataaca tctttatctg
    13981 tataagttaa attcaggaat tccaatgcct tttccgggtt tttacaggta gatgcaactg
    14041 cccatgatac tctgtttaca tcagaagttc caagatacgg ctggctcaaa cgaagagccc
    14101 ctattttctg gcctgtctga gtggagatct gggttgcggc gtcatttcca ccatagttgg
    14161 agatgtaaga aaatcctttt cctgcgctta acagctcaat ggatgtactg gtagtcgttg
    14221 ctgcgtcacc cataatatag cctttgttat accagtctct tgccagtgtc aggatatcct
    14281 taaatccatc tgtttcatag aaattctcaa cctgcatatc atcgccattt aaaattccag
    14341 ttggagataa atagcggtct cccagataat caaatccata aatggtattg atcatacctg
    14401 aatcacctgg attgaccggt accagcggga tcatatcagg ataagcctct tttacctttg
    14461 cgtatacatc agtcagatca ttaatatttt taatgtctgc cggatctacg ccgatctctt
    14521 ccatgatatc ttctctgtac agaagtgtcg gcgcaagagc aacaccctta tcagccggaa
    14581 taccataaac ctttccgtct actacttctg ttttcataaa gtcctcacct accgcgtcaa
    14641 tggcgccggc agcatgttcc tttacaatat ctgtaatgtc ataaagctga tgattggcaa
    14701 ggcaggtaga taactccaga ccgcttcctg tatggaacag gtccagctgt tccccgccct
    14761 gcattgcaag acttacctgc tggccatatg cagaagaagc aatcggcatt aattctactt
    14821 ccacaccgat cttctctctt gtgatctcac tgatcgcttc atttacgtcg gacagatctt
    14881 caggaatatt gttaaatgat ttaaaggcat atacgatctt tgtatattct tcatttcctt
    14941 ccgttttctg atctgtggca gtctgctgac cggctgtatt atccgaactg ctgccgcatc
    15001 cagtcatgga aaatgccatt gaaccggcaa gaagaacggc cgccgcgcct gcaactctgt
    15061 ttttcatact ttttttcata tcaagtaacc tcctttattt tgcctttctg ggctttatta
    15121 taaaggaggt ccatgataca tttctttaaa aaataagtga tatttttttg attttaagat
    15181 ttttttgatc tgcggtattc tcccggtgtg atcccggtat actttttaaa tacagtagaa
    15241 aaataagaaa aattgtcaaa acccacttct gctgaaatat cactgatgct ctttatatca
    15301 tcagccagaa gctgctttgc catttcaatg cgataaccga tgatataatc ctttaaatta
    15361 tgtcctgtct gccttttata gaccttggca agatattccg gagtcagaaa gaacacatct
    15421 gcaatagtgt ctctggaaat gtcctcttta taatgttctt tcacatagct ttcgatctta
    15481 tctgccagag aaagaggccg gttgatctca tcttcatact ccagagtacg tgatactgtg
    15541 taattcaccc attttatcat gtcaaatacg gagcggactg actgccgctg gattttctgg
    15601 gaaacttcat ccccgaataa ctccgccgcc tgaatcccat tctgataaag attggtatag
    15661 atcagctgca ggatctcctg ctgtatgacc agcagcgttt ctactgttac gctcttttca
    15721 cgggaaagtc ttcccaaaac tgtctttacc gtatttaata tcttcatccg gtctttgctt
    15781 cgtagatcat tttccatttg cttcagatca agaagcctgg tgttaacccc tgatgtaact
    15841 gctgcttccc cttcatgaaa tacctggtca gactccagta catttcgcaa tagcagattg
    15901 atcacctcat ctgctttatc aggaagctgt tccatactgc atggatttcc aatacaacaa
    15961 gtaaaacagc agccaacata atcttgtccc gtttttatca gtatatggca tctttccatg
    16021 agttcctctt ccaaagtccg tttccagtcg atcaccagaa agaacaatgc ctcatttacg
    16081 atctttttta taaccgatgt attttcaaca gactcgtcca cgatctctga aaccattttt
    16141 tccagagcaa attccagcag gttttccccc agtgcttctt tagcctgttc ctctctgtca
    16201 actcttattc caatcagtcg taccggctga tctgcaaaaa caggaagacg gcgttttttt
    16261 atctcacggt caataaactc cgattctgcc cttaattccc gctctatcaa agcacgccag
    16321 aaattattca tcagctgctc ctgattattt ctcatccatt ttccataatg gctttcttct
    16381 tctattttcc gcttctccat gatcctctgg accaggcgta aaagttctgc ttctacctgc
    16441 tctacacgga atggttttac cagataacca gccgcctgat agcggattgc cacagacgcg
    16501 tactgaaatt ctgtatggca ggtgagaaac agaaattccg aagacatatc attttcccgg
    16561 acccacttaa gcagttctgt tccatttccc ataggcattt caatgtcaca aactacaata
    16621 tccgcaggta tatctgctaa catctctttt gccttttcaa tgttataagc ggtcatgacc
    16681 ttatcaatgt gcaaagcgct ccagttcacg gatttgcaga tcacatctac agttgccatg
    16741 tcatcatcca caatcaatac gttcattttt tatctcctgt tcctcaccca ctgctgttgt
    16801 tttggggatc catatctcga tcagacatcc ccctgtttcc aaatttgaca agtggatcaa
    16861 ttcccttttc tgatacaaaa cttccatggt tctttttatg ttccacagcc ctacatgatg
    16921 tccctccctc gaaggatctt cgctctcaaa attcatatac tgcataacct gttcaggaaa
    16981 acctacgccg tcatcctcca tacgaatata aagcatttct tccccctctt ttaaaaggac
    17041 ttttgcctgt atcagtatcg aaaggacgct gtcaattttt actgcatatt tgtattcatt
    17101 ttccacaaat gtatgtaaca gcatctgggg gatcttccac gacattgcag cttcttccat
    17161 atcaaaatac cagaatacac agtccggata ccggagctgc tgcatttcaa aataattttc
    17221 cagatgctgg gtttcttcct taagaaggac cagacgaaga ccgcctttaa acatataacg
    17281 gaccgttttt gttaatgcgt caatatatgt ccgtatatcc tcattccgct tctgataact
    17341 catactgtag atcgttgtca gggcatttaa aaagaaatgg ggttttaact gcatctgaaa
    17401 atatttcatc tgcgtatcca ttaactggat cttttcatca taggccctaa tccgaagatt
    17461 aacgatcaca ttcatcatcc ggttaaatgc ctgtgtaaga cgctgaagtt cctttgtatg
    17521 ataatcttcc tctgccctct gcttgtaatc tccgtcttca atctcctgga tcgtctgcat
    17581 cagatgatcc aggggcctgt ggatctcacg gtgaatataa tgtaaaagga tcatatcaca
    17641 gccaatagcc agaaccatga tcaaaaggat cagaatacta tttgtactga attttcccag
    17701 aaaatgatta ctgccgatca ttcctgtaat ataaagtcct gcacgatcac ttttaagaga
    17761 gatcatccgc tttttatgat cattcttcca ggaatcttct atttccaaag tctctcccag
    17821 atacatgctg tttccttctt ccaggttttc cacacataga ccatttacat
//
LOCUS       DBMDUA010000039        17843 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1864_length_17843_cov_3.593451, whole genome shotgun sequence.
ACCESSION   DBMDUA010000039 DBMDUA010000000
VERSION     DBMDUA010000039.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 17843)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17843)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17843
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1864_length_17843_cov_3.593451"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            139..1764
                     /locus_tag="ACZPCL_04850"
     CDS             139..1764
                     /locus_tag="ACZPCL_04850"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1161839.1"
                     /translation="MRKLDMAWINGNIYTADDKFSTAAAFGVSGDRFSVVGSTEEVLE
                     NCDEHTKIIDLQGKTVLPGLIDAHLHITNTGAMKLNLNLVSKQKQEILDMVADAYSSA
                     KKGEWIIGRGWINDTWDDPSFPTKEDLDAVAPDSPVYLTRACGHAAWANTKAFETAGI
                     TKDTPDPAGGEFLHKQDGSLLGVVTDQAQDPFNKAIPSYTKEQIQKIVLLAQQEFFSN
                     GLTSVQDAGSPEEWVEAWQELYEKERLKLRIYVSMRVLGRPNYQELMEGSLKFFKKGL
                     RIGMYDNRLTARAYKISLDGSLGARSAWMLEDYEDQPGHKGNGKWTNDQLYSVLYEAR
                     RAGFQTWCHAIGDAANRQCLDVYEQLLKELPDKDARLRSEHAQIIAPEDIERFRKLGV
                     IPTHQTVFLRTDKNVADARLGAERIKGAYAWRTLIDQGNPLPNGTDSPVESCNPFLSM
                     YCAVTRKDEHLKPEGGWHPQEAMTREEALRSYTNWAAYAAFEEQLKGSIEPGKLADFV
                     VIDRDYMKCPEEEIKDICSLQTVVGGEVVYETK"
     gene            1778..3349
                     /locus_tag="ACZPCL_04855"
     CDS             1778..3349
                     /locus_tag="ACZPCL_04855"
                     /inference="COORDINATES: protein motif:HMM:NF015746.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HIR1161840.1"
                     /translation="MEKTKEEKKGLFFRFINGVEVIGNKLPHPFYLFTILIVVAIVLS
                     VIFAGVSVTYESASSKGTGGEIVTVTIKNLLNKETINYVTQNLYSIYYNFSPMMMMGL
                     LMLSIGLAEQVGLFGAFIKRTIAGVRPAFVFAIVSFIAINANTASNAGILGCTAVAAA
                     VFASMGYNPWLGIILAYAAGNAGMSANVFVGNLDVLLSGINESVCSSLGIDTSTVHVL
                     QNWYFMFACAIILTVVFTVVTTKFVRGFIGEAKDLSLIQIGSSDKDLSPLEHKALRNT
                     AIAAVIYLALLVIICIPQNSVFRADDGSLVPNSPLLKSVLTLLFLFFMVTGIAYGRTT
                     GALKDWNSLPKMLAGSINSMVNFMVIALPASLFIYLFNASNIPTALGAAGAEWIKTSG
                     INGFGLIVFIVFFSTFLNLFMTSGSSKWMILAPILIPMLYAIGFSPALTTVAYRIGDS
                     ATNAIAPISSDVALIVGLLGKYNTDKSKTPGMGTVFAGCMPYAIATMITELLILGVWW
                     AFNLPLGPGVTMFIK"
     gene            3367..4494
                     /locus_tag="ACZPCL_04860"
     CDS             3367..4494
                     /locus_tag="ACZPCL_04860"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR1161841.1"
                     /translation="MEMPVFTIRGKNHRERGVDYGVQCKELIQGVLERYIRFFNGGHT
                     AVSWDEAKVISKEFLPFIHSYSPELLDELEGIAQGADVEFDDLLTLNSRSEAMTLVRR
                     PPSDKEELDGCSSVAVLPDASANGHTILAQNWDTYTWQEYGTVILQILRDDGPDLMIV
                     TEAGQLARYGMNQAGIALGVNSLQKTYNPEVFGIPSVFIRRKFLEQDRYVDAVNQIFG
                     AESMLPMYYVAAYCGGDAMGFEKLKDGQLVLYPENGLIAHSNHIRHPHYAYQVDALGG
                     TLYRDRRILKHLQPKAGAVTMEDIKDAFKDHFGYPFSVCRHGDSRKTELNRISTLGCI
                     LMDVTDRRIWVCKGTPCCGEFKEYMWERPHKKSDWDQIEKW"
     gene            4502..5365
                     /locus_tag="ACZPCL_04865"
     CDS             4502..5365
                     /locus_tag="ACZPCL_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1161842.1"
                     /translation="MKDFRAVKTELACYAVIIFAAFLMGFAIKNLYDPVNLVTGGVSG
                     VAIIFKSLFDIPLWLTNTLLNIPLFLAAWRLKGWNFIKRTFIATVSLSVSLYVLPEMT
                     IAGDDIFLSALFGGILSGVGTGILFAVHATTGGTDMLAALLHLKIRQYSVSQIMQVLD
                     AMVVLAGASVFGLGSALYAMIAIYAVCRVSDGILDGLNFSKLAIIISERSKEIAGSIM
                     KNMERGVTAVKSVGMYSGTERDMLFCVVPRKEIIWLRDIVREYDKQAFLIVSDVKEVF
                     GEGFIEDISSK"
     gene            complement(5651..7207)
                     /locus_tag="ACZPCL_04870"
     CDS             complement(5651..7207)
                     /locus_tag="ACZPCL_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1161843.1"
                     /translation="MSILNVEHLSHGFGDRAIFQDVSFRLLKGEHIGLVGANGEGKST
                     FMNIITGKLMPDEGKVEWAKNVNVGYLDQHTVLEKGMTIREVLKSAFAPLFEMEERMN
                     HICDNMGDADEDQMNAMMEELGTIQDLLMAHDFYVIDSKVEEVGRALGLDEIGMDRDV
                     TELSGGQRTKVLLGKLLLQKPDILLLDEPTNYLDVQHIEWLKRYLQEYENAFILISHD
                     IPFLNSVINLIYHMENQRLDRYVGDYDKFQEVYSVKKAQLEAAYKRQQQEIAELEDFV
                     ARNKARVSTRNMAMSRQKKLDKMEVIELAKEKPKPEFHFLEARTPGKYIFETKDLIIG
                     YDEPLSRPLNLTMERGQKAVLVGANGIGKTTLLKSILGLTPALSGSVELGDYLSIGYF
                     EQEMAPGNTTTCLDEIWKEFPSYTQYQVRSALAKCGLTTKHIESQVRVLSGGEQAKVR
                     LCKLINRETNVLLLDEPTNHLDVDAKEELKRALKAYKGSILLICHEPDFYEGLATDVW
                     DCRDWALRLS"
     gene            7424..8038
                     /locus_tag="ACZPCL_04875"
     CDS             7424..8038
                     /locus_tag="ACZPCL_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161844.1"
                     /translation="MEKQKIKIVRKNDEYSAEYQVGDVFQVDSTWYGGVNVTSASGIP
                     LSLDKDEYVLLEEAEKEQPIDNYSYQCGVMDCFCEMVASGLKKLAMSHPCDTKAERDS
                     YLPQVKKLCEKYGILYHPQDEALITDLFPAEANQDKYNYLFFRTQDVYETYLELKKRQ
                     KELENQCGGTEEERYQLAADFGALLSYPEEGIRRLIEKTREARR"
     gene            complement(8079..8744)
                     /locus_tag="ACZPCL_04880"
     CDS             complement(8079..8744)
                     /locus_tag="ACZPCL_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1161845.1"
                     /translation="MDYKLLENAPLFKDVPPEEINVMLQCLGTEERDYDKGETIFHAG
                     DVIHNIGLVLTGRVQIENDDVWGNKSVMESVGPGFAFAESYACLPGEPLMVSAIAAEP
                     SHILFLDTGRVLTTCPSACTHHNRLIQNLLYISSQKNLNLSRRIFHTSSKSIRGRLLS
                     YLSFQASKAGSRDFCIPFNRQQLADYLSIDRSAMSAELGKMRKEGILETDKNHFVLKT
                     DLL"
     gene            8828..9553
                     /locus_tag="ACZPCL_04885"
     CDS             8828..9553
                     /locus_tag="ACZPCL_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1161846.1"
                     /translation="MIRRIVHIDEEKCNGCGLCAQACHESAIGMVNGKAKLLRDDYCD
                     GLGDCLPACPAGAISFIEREAAAYDEAAVLENKKKKAAAAHQAPAGGCPGTRMRQMNR
                     RSEETAQEGENTSSAAPVTSQLSNWPVQIKLAPIKAPYFDQAKLLIAADCTAYAYGNF
                     HKDFVKGKVTLIGCPKLDDCDYSEKLTEIIAANDIKSVTLVRMEVPCCGGLEQAAIKA
                     LKNSGKFIPWQVVTISIDGKILD"
     gene            9620..9964
                     /locus_tag="ACZPCL_04890"
     CDS             9620..9964
                     /locus_tag="ACZPCL_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR1161847.1"
                     /translation="MAFIKNIEHETVLPLAEQVTVQPGQIVSRTLAQNKAVSVTLFAF
                     DKGEEISTHDSTGDAMVQVLEGKGKFTVGGKEHFCCAGDVLIMPAKVPHAVYAEESFK
                     MLLTVVFPTEQE"
     gene            9969..10481
                     /locus_tag="ACZPCL_04895"
     CDS             9969..10481
                     /locus_tag="ACZPCL_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161848.1"
                     /translation="MESAEKKRPGDILTGALIGLARTTYSHLKTEDTDRVVTCALSVL
                     ARPDAGEEELAQQLKQVRAEKLAVAPGCATCVAKCGNTDDYDMERLWNGPDQVRSLKI
                     CLFSGLKKTGRDAFKVIENGKGKDKHFQSIMESIYKGLFILAEDWEPEEILPAIAELD
                     EAEIMVKEFL"
     gene            10623..12365
                     /locus_tag="ACZPCL_04900"
     CDS             10623..12365
                     /locus_tag="ACZPCL_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR1161849.1"
                     /translation="MPGRREENAGKRMLKHLSAILEKSGVIIFEYHIKTDTLIRYNKE
                     LEVEVEIPKYCDYLRDKTRIYPDDRWKAIEFYSGRIKGPIDIREVDDDGYVSRKRLET
                     VSIEEDEEEGRSCILFGMVTDVTGEWHQEERLERELGQYREEEATCNGCAGYPKYDQV
                     TGLLSFNRFREEVDSVIASGKGKSCLLVYTDLENFKYFNRKYGYAAGDQLLREFTGYI
                     IDTLKKRNTAHFTRVVADQFILFFEEKWNNGSIEAIKRINNGFIERQQRKYPEAKLRL
                     RTGVYKVEKDCDGASFAIDAANYARRQIQDNSPETVCVYDKTMADLQNMENEIAIGLD
                     KAIKNKEFKIYLQPRFSLTTGKIVGAESLVRWQREDGTILPPDSFIPVYEKNGRIIDL
                     DFYMFEETVKLLANWKEQGIPQRLISINASALHAQNSDTVHRYTTILEKYGVDPALIE
                     LELTETATVSDYDHVKQLFAKLQTVGFRTALDDFGAGYSILNTVIDIPVNTVKVDRAF
                     ISNCETTHKGIYFLREIINLLKGLGYHIVCEGVETPEQASILRNTMCDEVQGYWFSHP
                     VPAHEFEKMLQEGR"
     gene            complement(12375..12590)
                     /locus_tag="ACZPCL_04905"
     CDS             complement(12375..12590)
                     /locus_tag="ACZPCL_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024934.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1161850.1"
                     /translation="MLCDQPDGLLYKINLTRQQLQDETSINVKTLNRTISQLKEEGFI
                     SLEKGKICYYKENYKKAVTWLQEARRR"
     gene            12624..13280
                     /locus_tag="ACZPCL_04910"
     CDS             12624..13280
                     /locus_tag="ACZPCL_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1161851.1"
                     /translation="MNEYPGEVNFFLQRFQKRPYEYLDSFHVLSDRFIGAHSIMVSAH
                     ERALLRERDVKVCHCPFSNCGKGVPETPALLEDGICVGLGTDGAAHGGLSLWNEMKIF
                     RSVMNVKHGVAQSNPAIMSAAQIISMATKEGYRLMKEEGGVIAEGKKADFITINTMQS
                     HLYPTGNLVNTLLECVTASDVCDMAVDGRLLMKDREVLTLDEEKILYEAMKYMEELEA
                     "
     gene            13277..13513
                     /locus_tag="ACZPCL_04915"
     CDS             13277..13513
                     /locus_tag="ACZPCL_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161852.1"
                     /translation="MITGITFCLIVTMANLVVGAFVGLTGVAGFLLPITYTSLLGLGT
                     TEGLALSFGAFILSGHAMHEQEAAFLIRPYWKWG"
     gene            13545..14501
                     /locus_tag="ACZPCL_04920"
     CDS             13545..14501
                     /locus_tag="ACZPCL_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HIR1161853.1"
                     /translation="MNVLYRKTTKGICVGRCYTLDNKLVIPDTIEGLPVTELDTYTFS
                     QTVRRREQPPAEYEGEPEMCGEALEELTMPKHLEKVGAYAFYNCFHLKNLSCCSTVRD
                     WGAGVFTGCTQINKLDIRIFPEEKSSFKEILSELRQMLVVDYRDKEGKLLAKLIFPEF
                     FEESIENTPARIIMREMHGCGHMYRYCFNGTDFQFWEYDKLLPTAEILESPALVCRMA
                     LYRLYWPKGLTEEWKEEYWKYIKKHPDEAAKGLTERGEREILSWLAEAKETDSQMLEQ
                     MIQATAGLGDAQVSAILMDARHKKMGAQSGDKPKPRVRTFEL"
     gene            14612..16009
                     /locus_tag="ACZPCL_04925"
     CDS             14612..16009
                     /locus_tag="ACZPCL_04925"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR1161854.1"
                     /translation="MSKKTKAQELQEQLTWSAPHIGKDAPDHKEKAFKYCEGYKQFLN
                     AGKTERECVKEAVHMLKKAGYKPFDRTASYEPGDKVYYVNRRKAIIATTFGKKPLSEG
                     LHINGAHIDSPRLDLKPNPLYEKEDIAYFKTHYYGGIRKYQWGTIPLAIHGVVAKKNG
                     ELTEICIGEKEDDPVFCITDLLPHLAAKQNERPLREGLKGEELNVVLGSLPYEGEDIK
                     DAVKLSILALLHEQYGMKEKDFFRAEFELVPAVKARDIGLDRSLVGAYGQDDRVCAYT
                     ALTAEIDTKKPEHTTVTILTDKEETGSDGNTGLNSDYVLHYIEDLASMEKVAVRDVLK
                     ASLCLSSDVNAAYDPTFPDVYEARNSSYINKGCVLTKYTGARGKGGSNDASAETMAKV
                     IGIMEDAGVYWQAGELGAVDAGGGGTIAKFVAHMDVDTVDLGVPILSMHAPFELASKL
                     DVYHTYKAFKAFYAS"
     gene            16161..17306
                     /gene="tig"
                     /locus_tag="ACZPCL_04930"
     CDS             16161..17306
                     /gene="tig"
                     /locus_tag="ACZPCL_04930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR1161855.1"
                     /translation="MRKLKLLCLCGLTAAVLMTGCAGSTDTTNVNLGSATVSEYRGVK
                     INVAAPEVTDDEVDQKIQTNLKQNPNEVEVDRAAQDGDIVNIDYKGTKDGEAFDGGTA
                     EGQDLTLGSGSMIDGFEDGLVGAKKGETKTLDLTFPENYREESLAGQAVVFEVTVNAV
                     KEKQDAVLDDAFVQRTSDYQTVDEYKEGIRADLLAQKQKTAEQEMEQDVLQDIIDNSD
                     FKFSRNGLSVRYNQMLKQYTNQAKMYGMTLSQMAQANGMDEAGFKEYIYSSVKEAAKK
                     EIVVKDIAAKEGLDNLTDEDKEAFAQANGTSKDTLVSLYGEDTVNEQVLQDKVLRFLA
                     SNADNEAENPAKLSEREVTVTETSADQESSPEETTEAETTAEETKAN"
     gene            17348..>17843
                     /locus_tag="ACZPCL_04935"
     CDS             17348..>17843
                     /locus_tag="ACZPCL_04935"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HIR1161856.1"
                     /translation="MQLLKDRIRKDGKIKAGNVLKVDSFLNHQMDIKLFGEIGKEFKR
                     RFADVEVNKILTIEASGIGIACIVAQYFDVPVVFAKKTQTKNIAGEVYTSKVESFTHG
                     RVYDIIVSKEFLGKGDKVLLIDDFLANGKALEGLAALVRDSGAELVGAGIVTKELLTP
                     KGLRT"
BASE COUNT         5279 a         3517 c         4391 g         4656 t
ORIGIN      
        1 agtcgcaccc gaattttatc aacgtacgca ccgcgtacgc ctcaaaaatt tgggcacaac
       61 tgacaaaaaa tcttctcgca aaatcaagta caaaaagatt ccgagagcac atatgtaaaa
      121 acaggaggag gaatcaagat gcgaaaactg gatatggcat ggatcaacgg gaatatttat
      181 actgcagatg ataaattttc cacagccgct gcattcgggg tgtcggggga cagatttagt
      241 gttgttggca gtacagagga agttcttgaa aattgtgacg aacacacgaa gatcatagat
      301 cttcagggaa aaacggtact gcctggattg atcgatgccc atcttcacat taccaataca
      361 ggtgctatga agctgaacct gaatctggta tcgaaacaaa agcaggagat cctggacatg
      421 gttgctgatg cttacagcag tgcgaaaaaa ggagaatgga tcattggacg gggatggatc
      481 aatgatacgt gggatgatcc atcatttcct accaaagaag acctggatgc tgtggcgccg
      541 gattcaccgg tataccttac aagagcctgc ggacatgctg catgggcgaa tacaaaggca
      601 tttgaaacag cagggatcac aaaggataca ccagacccgg cagggggaga atttttacat
      661 aagcaggatg gaagcctcct tggtgtggtt actgatcagg cgcaggatcc atttaataaa
      721 gcgataccat cgtatacaaa ggaacagatc cagaagatcg tattgctggc ccagcaggaa
      781 ttcttttcaa atggtttaac atctgttcag gatgccggct caccggaaga gtgggtggaa
      841 gcatggcagg aattatatga aaaagagcgt ctgaaattaa ggatctatgt aagtatgcgt
      901 gtactgggac gtccgaatta ccaggaactg atggaaggct ctttaaaatt ctttaagaag
      961 ggactccgca ttggtatgta tgacaatcgt cttacagcca gagcctataa gatatccctt
     1021 gatggatctt taggagccag aagtgcatgg atgttagagg actatgaaga ccagccaggt
     1081 cataaaggaa atggaaagtg gacaaatgac cagttgtatt ctgtattgta tgaggcgcga
     1141 agggctggtt tccagacctg gtgccatgca attggagatg cagctaaccg tcagtgtctg
     1201 gatgtatatg aacagctttt aaaggagctt ccagataaag atgcacgtct tagaagtgag
     1261 catgcccaga tcattgcccc ggaagacata gaacgcttcc gcaaactggg ggttatacct
     1321 actcatcaga ctgtattttt acggacggat aaaaatgtag cagatgcacg tctgggagca
     1381 gagcgcataa aaggtgcata tgcatggaga acattgatcg accagggaaa tcctttacca
     1441 aacggaacgg attcaccggt agaaagctgc aatccatttt taagcatgta ttgtgcggta
     1501 acaagaaaag atgagcattt aaaaccggaa ggaggatggc atccacagga agcaatgacc
     1561 cgggaggaag cactgcgttc ttacacaaat tgggcggcct acgcagcatt tgaagagcag
     1621 ttaaaaggca gtatcgagcc gggaaaactg gcagattttg ttgtgatcga ccgtgactat
     1681 atgaaatgcc cggaagaaga aataaaagat atctgctctc tgcagacagt tgttggcgga
     1741 gaagttgtat atgaaacaaa gtaaaggaga atcaggtatg gagaagacaa aagaagaaaa
     1801 aaaggggttg tttttccggt ttattaatgg tgttgaggtt attggtaata aacttccgca
     1861 tccattttat ctgtttacga tcctgatcgt ggtggcaatt gttctttctg tgatatttgc
     1921 aggtgtttct gtgacttatg aaagtgcatc ttccaaagga acaggcgggg aaattgtaac
     1981 agtgactata aagaacctgt taaataaaga gaccataaat tatgtaacac agaatttata
     2041 cagtatttat tataactttt caccaatgat gatgatgggc ctgctgatgc tgtccatcgg
     2101 actggcagaa caggtgggac tgttcggtgc gtttattaag agaacgatcg caggtgtacg
     2161 tccggcattt gtatttgcca ttgtttcatt tattgcgatc aatgcaaata cagcctctaa
     2221 tgcaggtatc ttaggctgta cagcagtagc agcggcggta tttgcttcaa tggggtataa
     2281 tccgtggctg ggcatcattt tggcatatgc ggcaggtaat gccggaatgt ctgccaatgt
     2341 atttgttgga aatctggatg tactgttaag tggtatcaat gaatctgtat gttcttctct
     2401 gggaatcgac acctctactg tacatgtact tcagaactgg tattttatgt ttgcatgcgc
     2461 cattatcctg acagtggtat ttaccgtggt aacaacaaaa tttgtcagag gctttatcgg
     2521 cgaagcaaag gatttaagtc ttattcagat tggctcttca gataaagatt tatctcccct
     2581 ggagcataag gcactccgga atacagcgat cgcagcagtt atttacctgg cattactggt
     2641 catcatatgc attccgcaaa attctgtttt ccgtgcagat gatggaagcc ttgttcccaa
     2701 ctctccgtta ttaaagagtg tgttaacact gttattttta ttctttatgg ttacaggaat
     2761 tgcctatgga cgtacaactg gtgctttaaa agactggaat tccctgccta agatgcttgc
     2821 aggcagtatt aattccatgg ttaatttcat ggttatcgca cttccggcct ccttatttat
     2881 ttacctgttt aatgcatcta atattccgac tgcattaggt gctgcaggcg ctgaatggat
     2941 aaaaacatca ggtatcaatg gatttggcct tattgtattt attgtatttt tctctacatt
     3001 tttaaattta tttatgactt ccggatcttc aaagtggatg atcctggcac cgatcctgat
     3061 cccaatgctt tatgcgatcg gctttagccc ggctttaact acagtagcat accgcatcgg
     3121 agattctgct accaatgcga tcgcaccgat ctcttctgat gtggcactga tcgtaggact
     3181 tctcggtaag tataatacag acaagagtaa aacaccgggt atgggaacgg tttttgcagg
     3241 ctgtatgcca tatgcaatag cgactatgat cacggaactg ctcatattag gtgtctggtg
     3301 ggcatttaac ctgcctcttg gccctggtgt tactatgttt atcaagtaaa gagaggagag
     3361 aataatatgg agatgcctgt tttcactatc cgtggtaaaa accacagaga acgtggagta
     3421 gattatggag ttcaatgtaa agaactgata cagggagtat tggagcgcta tatccggttt
     3481 tttaatggcg ggcacacggc ggtaagctgg gatgaagcaa aagtgatatc caaagaattt
     3541 cttcctttta tccattctta ctcaccggaa cttttagatg aattagaagg gatcgcccag
     3601 ggcgcagatg tagagtttga tgacctttta accttaaatt cacggtcaga ggctatgact
     3661 ctggtacgca gacccccttc tgataaggag gaactggatg gctgttccag cgttgcagta
     3721 ttgccggatg cttctgctaa tggacatacg atcctggcac agaactggga cacttacaca
     3781 tggcaggaat atggcaccgt gatcttacag attcttagag atgacggtcc ggatctgatg
     3841 atcgttacag aggcaggaca gcttgccaga tatggcatga accaggcagg aattgccctg
     3901 ggagtcaaca gtctgcaaaa aacatataat cctgaagttt ttggaatccc atctgtattt
     3961 atccgccgta aatttttgga acaggatcgt tatgttgatg cagtgaatca gatattcgga
     4021 gcggaatcga tgcttccaat gtattatgtg gcagcttatt gcggtggtga tgccatggga
     4081 tttgaaaaac taaaggacgg acagctggtg ctgtatccgg aaaatggcct gatagcccat
     4141 agtaaccata tccgccatcc tcattatgca taccaggtgg atgctctggg gggaacttta
     4201 tatcgtgaca ggagaatttt aaaacatctt cagcctaaag caggtgcagt caccatggaa
     4261 gatataaagg atgcttttaa ggatcatttt ggatatcctt tcagtgtttg cagacacggt
     4321 gatagcagaa agacagagct gaaccggatc agtacgcttg ggtgtatcct tatggatgtg
     4381 acagaccgcc gcatctgggt atgtaaagga accccatgtt gtggggaatt taaagaatat
     4441 atgtgggaac gtccgcataa aaaatctgac tgggatcaga tagaaaaatg gtgaaataaa
     4501 aatgaaggat ttcagggcag taaaaactga attggcttgt tacgcagtga ttatttttgc
     4561 agcattttta atggggtttg caataaaaaa cctttatgat cctgtgaacc ttgttaccgg
     4621 cggagtatcc ggcgtggcga tcatattcaa aagtctgttt gatataccct tgtggctgac
     4681 aaatacattg ctgaatattc cattatttct ggctgcctgg cgtttgaaag gatggaattt
     4741 tattaaaagg acatttatag caactgtgtc cttgtcagtt tctttgtatg ttttgccgga
     4801 aatgaccatt gccggagatg atatcttttt atcagcttta tttggcggta ttctttccgg
     4861 agttggaaca gggattttat ttgcagtaca tgccactaca gggggcacgg atatgcttgc
     4921 agccctgctt catctgaaga tccgtcaata ttctgtatct cagatcatgc aggtccttga
     4981 tgcaatggta gtactggcag gtgcttctgt atttggactg ggatcagcat tgtatgcgat
     5041 gatagcaatc tatgcagtgt gccgcgtatc agatggaatt ttagacggtc tgaatttttc
     5101 aaagcttgca atcattattt ctgaacgcag taaagagatc gcaggctcga tcatgaaaaa
     5161 tatggagaga ggtgttactg ctgtaaagtc agtaggaatg tattccggga cagaaaggga
     5221 catgctgttt tgtgttgttc ccagaaaaga gatcatctgg cttcgggata ttgtccggga
     5281 atatgacaag caggcgtttc ttattgtaag tgatgtgaag gaggtattcg gagaaggttt
     5341 tattgaagat atttcttcaa aataaaagat aatcagaaat aacagaaggc tgttttcagg
     5401 tcattcaatt taaacggaca tgcctgctga cgcaggcatc accattcatg aaagccgatt
     5461 gctccgcacc tttggtgctc ccgcaatcgt cgccagcatt cgcaatccgc gctatcgcgc
     5521 tccttgctca taccggcttg cccgtccgat acccgtgaat tcgctcatgc aagcatggct
     5581 cattcgcggc tgccgtccgg gactttcata gtaaatgatc tgaaagcagc cttttatgat
     5641 catggtaaga tcatgataaa cgcagtgccc agtctctgca gtcccataca tctgtagcca
     5701 gaccttcata aaaatccggt tcatggcaga tgagaaggat gcttcctttg taagccttta
     5761 aggcacgttt taattcttct tttgcgtcta catccagatg gttagtaggt tcgtctaaaa
     5821 gaaggacatt ggtttcacgg ttgatcagtt tgcacaggcg cactttggcc tgttcaccgc
     5881 cgcttaatac gcggacctgg ctttcaatgt gttttgtggt cagaccgcat tttgcaaggg
     5941 cagagcgcac ctgatactgg gtataagacg ggaattcctt ccagatctca tccagacagg
     6001 tagtcgtatt gccgggagcc atttcctgtt caaaatatcc gatggacaga taatcgccca
     6061 gttccacaga accgcttaaa gcaggtgtca gtcccaggat actctttaaa agagtggttt
     6121 ttccaatacc gtttgcacct accaggacgg ccttctgtcc gcgctccata gtcaggttca
     6181 aaggacggga aaggggctcg tcatagccga tgatcagatc cttggtctca aaaatatatt
     6241 tgcctggagt gcgggcttcc aggaaatgaa actctggctt tggcttctct tttgccagtt
     6301 cgatcacttc cattttatcc agtttcttct gacgggacat agccatattt ctggtggaaa
     6361 cgcgggcttt gtttctggca acgaaatctt ccagttcagc gatttcctgc tgctggcgct
     6421 tataggcagc ttctaactga gccttcttta cagaataaac ttcctggaat ttgtcgtaat
     6481 cgcctacgta gcggtccaga cgctggtttt ccatgtgata gatcaggttg ataacgctgt
     6541 ttaagaatgg gatatcatgg gagatcagga taaaagcatt ttcatattcc tgaagatagc
     6601 gctttaacca ttcaatgtgc tgtacgtcta ggtagttggt aggctcgtca agaagcagga
     6661 tatccggctt ctgaagcagc aactttccaa gaaggacttt ggttctctga ccgccggaaa
     6721 gctctgttac gtcacggtcc ataccgatct catccagacc taaagcccgg ccaacttcct
     6781 ctactttgga gtcgatcaca tagaaatcat gtgccattaa aaggtcctgg atggttccaa
     6841 gctcttccat catggcattc atctgatctt cgtctgcatc ccccatattg tcacagatat
     6901 ggttcatccg ctcttccatc tcaaaaagag gagcaaaggc gctttttaat acttcccgga
     6961 tggtcattcc tttttccagg actgtgtgct ggtccagata gcctacattt acgttctttg
     7021 cccactctac cttgccttca tccggcatca gctttccggt gataatgttc ataaaagtgg
     7081 atttaccttc accgttggca cctacaagac caatgtgttc tccttttaaa aggcggaaag
     7141 acacatcttg aaaaatagcc cggtctccaa aaccgtggct taaatgttct acatttaata
     7201 tactcattca aaaactcctt ataaaataaa tatgctctcg gaaacttcca agagtacata
     7261 tgatcaacag tagcacagat aagcccattt tacatgagaa atcaggctgt ggcaaccgct
     7321 ttttcactgc tcttgaggtt tgtttgggaa tgtgttaaga tggcgtcaaa caggatttat
     7381 gtgaatgcaa aagataagtt acaacaagga gataaaacta cttatggaaa aacagaaaat
     7441 aaagatcgtt cgcaaaaatg atgagtattc tgcggaatac caggttggtg acgtttttca
     7501 ggtggacagt acctggtatg ggggagtgaa tgttacaagc gcgtcgggaa taccattgtc
     7561 tttggacaag gatgagtatg tgcttttaga ggaagcagaa aaggagcagc ccattgacaa
     7621 ttattcctat cagtgtggtg tgatggactg cttctgtgaa atggtggcgt ccggccttaa
     7681 aaagctggca atgagccatc catgtgacac caaagcagaa agagactctt atctgcccca
     7741 ggtaaagaag ctttgtgaaa aatatgggat cctttatcat ccacaggatg aggcgctgat
     7801 cacagatctg ttcccggcag aggcaaatca ggacaaatat aattatttat ttttccgtac
     7861 tcaggacgtt tatgagacct atctggaact gaaaaaacgc cagaaagagc tggaaaacca
     7921 gtgcggagga actgaggaag aacgctatca gctggcagca gactttggag ccctgctttc
     7981 ttatccggaa gaaggaatac gaaggctgat cgaaaaaaca agagaagcca gaagataaaa
     8041 aagagtgcgc atcgttggca caaaaagcag acctgccgct acagcaggtc cgtttttaat
     8101 acaaagtgat ttttatctgt ctccaggatc ccttcttttc gcattttacc aagttccgcc
     8161 gacatggcgc ttcggtcaat gcttaaatag tcagctagct gctgcctgtt aaatggaata
     8221 caaaaatccc ggctgcctgc tttggaggcc tgaaaagaaa ggtatgataa caggcggcca
     8281 cggatggatt tggaagaggt gtggaagatc cgtctggaca gatttagatt tttctgggaa
     8341 gagatatata acaggttctg gatcaggcgg ttgtggtggg tgcaggcaga aggacaggtg
     8401 gtcagtaccc gtcctgtatc taaaaacagg atatgggaag gttctgctgc aatggctgat
     8461 accataaggg gttccccggg aagacaggca tagctttccg caaaggcaaa gccgggacca
     8521 acactttcca taacactttt attgccccag acatcatcat tttcaatctg tacccggccg
     8581 gtcagcacca gtccgatatt gtggatgaca tctccggcat gaaagatggt ctcacctttg
     8641 tcataatccc gttcctctgt gccaagacac tggagcataa cattgatctc ttcaggggga
     8701 acatctttaa ataagggtgc attttccaac agtttataat ccattttctc tccttttgtt
     8761 gtcagaacaa catacatttg caggtgattg tagtatattc cttatataat gtcaaggagg
     8821 tacaaaaatg atacgcagga tcgttcatat tgatgaagaa aaatgtaatg gctgtggcct
     8881 ttgtgcccag gcctgtcacg aaagtgccat tggaatggtt aatggcaagg ctaaattatt
     8941 aagagatgac tattgtgatg gtttaggaga ctgccttccg gcctgtccgg caggtgccat
     9001 ttcctttata gaaagagaag cagctgcata tgatgaggca gcagtacttg aaaataagaa
     9061 gaaaaaggca gcagcggccc atcaggctcc tgcaggcgga tgtcctggca cccgtatgcg
     9121 ccagatgaac cgcagaagcg aagaaacagc tcaggaagga gaaaatactt ccagtgcagc
     9181 accagttact tcccagttaa gcaactggcc ggtacagatc aaactggcac cgatcaaggc
     9241 accatacttt gatcaggcaa agcttttgat cgcagcagac tgtactgcct atgcatacgg
     9301 aaacttccac aaggattttg ttaaaggcaa agtaacactg atcggatgtc caaagttaga
     9361 tgactgcgat tacagcgaaa aactgacaga gatcattgca gccaatgata ttaagagcgt
     9421 tacgttagtg cgcatggaag tgccatgctg cggcggttta gagcaggcag ctatcaaggc
     9481 tttaaagaac agtggaaagt ttattccatg gcaggtagtg accatttcta ttgacggtaa
     9541 gatactggat taacgtttag gacatgtctc acgcccgatg ggcggggaaa agattagttt
     9601 gtagcaggag aaaactaaaa tggcatttat taaaaacatt gaacacgaaa ccgtacttcc
     9661 gttagcagag caggtaactg tacagccggg acagatcgtc agccgtacac tggctcagaa
     9721 caaggcagtc agcgtaactt tatttgcctt tgataagggc gaagagatca gtacccatga
     9781 ctccaccggc gatgctatgg tacaggtact ggaaggaaaa ggaaaattta cagtaggcgg
     9841 aaaagagcat ttctgctgtg ctggtgatgt cctcatcatg ccggcaaagg ttcctcacgc
     9901 tgtttacgca gaagagtcct ttaaaatgct gttgactgtt gtatttccaa cagaacagga
     9961 gtaaagatat ggagtccgca gaaaagaaac gaccagggga tatccttaca ggcgctttga
    10021 tcggacttgc caggacaact tacagtcatt taaagacaga agatacagac agggtagtaa
    10081 catgtgctct gtctgttctg gcaagaccgg atgccggtga ggaagaactt gcccaacagt
    10141 taaaacaggt aagggcagaa aaactggcag tagccccggg ctgtgctacc tgtgtggcaa
    10201 aatgcggaaa tacagatgat tatgatatgg agcgcctctg gaacggaccg gaccaggtgc
    10261 gctctcttaa gatatgtctg ttttccgggc tgaaaaagac aggcagggat gcttttaaag
    10321 ttattgaaaa cggaaaaggg aaagacaaac atttccagag tattatggaa agtatctata
    10381 aaggactttt tatcctggca gaggactggg aaccggaaga gatactgccg gctattgctg
    10441 aattagatga agcagagatc atggtgaagg aatttctcta aaaaaatatt ttagaaaatt
    10501 attggaaaaa tttggcaaaa atgccaagga agcattaagg gtattcaact tgaatagaaa
    10561 gtataagtat ggtaaagtag gaataatgat aaaagtactt aagaaaagag gaggtacaga
    10621 agatgccagg gcgcagggaa gagaatgccg gcaaaagaat gttgaagcat ctttccgcta
    10681 ttttggagaa aagcggtgtc attatatttg aataccatat aaaaacagat actctgatcc
    10741 gttacaataa agagctggaa gtagaagtgg aaataccgaa gtattgtgat tatctcaggg
    10801 ataagaccag gatctacccg gatgaccgct ggaaagccat tgaattttac agtgggcgta
    10861 ttaaggggcc cattgatatc cgtgaagtag atgatgacgg ctatgtttcc agaaagcgcc
    10921 tggagactgt atccattgaa gaagatgaag aagaaggtag atcttgtatc ctctttggca
    10981 tggtcactga tgtaaccggg gagtggcatc aggaagagcg cctggaacgg gaactgggac
    11041 agtaccggga agaagaggca acctgtaatg gttgtgccgg ttatcctaaa tatgaccagg
    11101 ttacaggtct gctttctttt aaccgtttca gggaagaggt tgacagtgtg atcgcttccg
    11161 gaaaaggaaa aagctgtctc ttagtttaca cagatcttga aaactttaaa tattttaacc
    11221 ggaaatacgg ttatgcagca ggagaccagc tgctgcggga atttaccggt tatattattg
    11281 atactttaaa aaagcgcaat actgcccatt ttaccagggt ggtcgcagat cagttcattc
    11341 tcttctttga ggaaaaatgg aataacggtt ccatagaagc cattaagcgt atcaataatg
    11401 gatttattga gcggcagcag agaaaatatc cggaagcaaa gctgcgcctg cgcaccggtg
    11461 tatacaaagt agaaaaagac tgcgacggag cttcgtttgc tattgatgcg gccaactatg
    11521 ccagacgcca gatccaggat aattcccctg agactgtatg tgtatatgat aagaccatgg
    11581 cggatctcca gaatatggaa aatgaaattg ccattggctt agataaagca ataaaaaata
    11641 aagaatttaa gatctatctc cagccacgtt tctccctgac aacaggaaag atcgtaggtg
    11701 cggagtcttt agtccgctgg cagagggaag atggtacgat cctgccgccg gacagtttta
    11761 tccctgttta tgagaaaaac ggccgtatca ttgacctgga tttttacatg tttgaggaaa
    11821 cggtaaagct tctggcaaac tggaaggaac aggggatccc gcagcgtctg atctccatca
    11881 atgcgtctgc ccttcatgcc cagaacagtg ataccgttca ccgttataca accatcctgg
    11941 aaaaatacgg tgtggacccg gctcttatag aactggaatt aacagagaca gctacagttt
    12001 ctgattatga ccatgtaaag cagctgtttg caaagttaca gactgtcggt tttaggacag
    12061 ccttagatga ttttggagcc ggttattcca tattgaatac agtcattgat attccggtca
    12121 acacagtaaa agtggaccgg gcctttatca gcaactgtga gactactcat aaggggattt
    12181 acttccttcg tgagatcatc aacctgttaa aaggtcttgg ttatcatatt gtatgtgagg
    12241 gagttgagac gccggaacag gcgtccatcc ttagaaatac catgtgtgat gaagtacagg
    12301 gatactggtt ttcccatccg gtcccagcac atgaatttga aaaaatgctg caggaggggc
    12361 ggtagcagag aatattatct gcgccgcgcc tcctgcagcc atgttacagc ctttttataa
    12421 ttttctttat aatagcagat cttgcccttt tccagggaaa taaatccctc ttcttttaac
    12481 tggctgatgg tacggtttaa tgtttttacg ttgatgcttg tttcatcctg caactgctgt
    12541 ctggtcagat tgatcttata aagaagacca tccggctgat cacagagcat gcagatgacc
    12601 gccaggctct tttacaggcc catatgaatg aatatccggg agaagtgaat ttcttccttc
    12661 agcggttcca aaaacgtcct tatgaatatc tggactcttt ccatgtttta agcgaccgct
    12721 ttatcggtgc ccacagtatc atggtatcgg ctcacgaaag agcactgctg cgtgagcgtg
    12781 acgtaaaagt atgccactgt ccgttcagca actgtggaaa aggagttccg gagacaccgg
    12841 cacttttaga agatggaatc tgcgtaggac ttggaacaga cggtgctgct cacggtggtt
    12901 taagtctttg gaatgaaatg aagatcttcc gttctgtgat gaacgtaaaa cacggcgttg
    12961 cccagagcaa tccggctatc atgtctgcag ctcagatcat ttccatggct acaaaagagg
    13021 gataccgcct gatgaaagag gagggcggtg tgatcgcaga aggcaaaaaa gcggacttta
    13081 ttaccatcaa taccatgcag tctcatttat atcctactgg taatctggtg aatactctgt
    13141 tagaatgtgt tactgcgtct gacgtctgcg atatggcggt agatggcaga ctgctgatga
    13201 aggatcggga agttcttaca ctggatgagg aaaagatcct ttatgaagcg atgaaataca
    13261 tggaggaatt agaagcatga tcacaggtat cacattctgt ctgatcgtaa caatggccaa
    13321 tctggtagta ggagcatttg taggacttac aggtgtggca ggtttcctgc tgccgatcac
    13381 ttatacatcc cttctgggac tgggcacgac agagggactg gcccttagct ttggtgcttt
    13441 tatcctttct ggtcatgcaa tgcatgaaca ggaagcagct tttttaatta gaccttattg
    13501 gaaatggggt tgaaaaaata agggaaactg attaaactga ttacatgaat gtattatata
    13561 gaaagacaac aaaagggatc tgtgtggggc gctgctacac attagataac aaactggtga
    13621 taccggacac catagaagga cttccggtaa cagaactgga tacatataca ttctcccaga
    13681 ctgtgcgaag aagagaacag ccgccggcag aatacgaagg agagccggaa atgtgcggtg
    13741 aagccctgga ggaacttacg atgccgaaac atctggaaaa agtaggggcc tatgcctttt
    13801 ataactgttt ccatttaaag aatctttcct gctgcagtac agtgagagac tggggggccg
    13861 gagtatttac aggctgtacc cagataaata aactggatat ccgtatcttt ccagaggaaa
    13921 aatccagttt taaagaaata ctttcagaac tgcggcagat gctggttgta gattaccggg
    13981 ataaagaagg gaaacttctg gcaaagctga tctttccgga gttttttgag gagtccatag
    14041 aaaatactcc tgcccgtatc attatgaggg aaatgcatgg ctgcggtcat atgtaccggt
    14101 attgttttaa cggaactgat tttcagttct gggaatatga caaactgctg ccaacagcag
    14161 agatcttaga aagcccggcc cttgtatgcc gcatggccct ttaccgcctc tactggccca
    14221 aaggtctgac agaggaatgg aaagaagaat actggaaata catcaaaaaa catccggatg
    14281 aagcagcaaa aggcctgaca gagcggggag aaagagagat cctttcctgg ctggcagagg
    14341 caaaagagac ggacagtcag atgttagagc agatgatcca ggctacagca ggactggggg
    14401 atgcccaggt ttctgccatt cttatggatg caaggcataa aaagatgggg gcgcagtcgg
    14461 gggataaacc gaagcccaga gtccgaactt ttgagttata gttttcaacg ggagttggtc
    14521 ccggttttcc cgctttagcg ggaagtatat actaatgttt ctctccgccc tcaggcggct
    14581 gagtattact taatttacgg aggaagatcc catgtccaag aaaacaaaag cacaggaatt
    14641 acaggagcag cttacctggt ctgctcccca tattggaaaa gacgcaccag accacaagga
    14701 aaaagctttc aaatactgtg aaggctacaa acagttcctt aacgcaggaa aaacagagcg
    14761 cgagtgcgta aaagaagctg tacacatgtt aaaaaaggcc ggttacaagc catttgaccg
    14821 cactgcttcc tatgaaccag gagataaggt atattatgta aaccgcagaa aagccattat
    14881 tgcaactacc tttggcaaaa agccattatc cgaaggtctg catatcaatg gagctcatat
    14941 tgactcccca agactggact taaagccaaa tccattatat gaaaaagaag atatagccta
    15001 ctttaagact cattactacg gcggtatccg caaataccag tggggaacta ttccgttagc
    15061 tattcacggc gttgttgcaa agaaaaacgg ggaactgacg gaaatctgca tcggtgaaaa
    15121 agaagatgac cctgtattct gcattacaga cctgctgcca catctggctg caaagcagaa
    15181 tgagcgcccg ctgcgggaag gcttaaaggg agaagaatta aacgttgttt taggttctct
    15241 tccatatgaa ggagaagaca tcaaggacgc tgtaaaactt tctatcctgg cactgcttca
    15301 tgaacagtac ggtatgaaag aaaaagattt cttccgcgca gaatttgaac tggtaccggc
    15361 tgtaaaagca agagatatcg gtttagaccg cagtctggta ggtgcttatg gccaggatga
    15421 ccgtgtttgt gcatacacag cactgactgc tgagattgat acgaaaaagc cggaacacac
    15481 cacagtgacc attcttacag ataaagaaga gacaggttct gacggaaata caggtctgaa
    15541 ttccgattat gtccttcatt atatagaaga tcttgcttct atggagaagg ttgcggtacg
    15601 tgacgtatta aaggcttcct tatgcttgtc ctctgatgta aatgcagctt atgacccgac
    15661 tttcccggat gtatatgaag cgcgcaattc cagctacatc aacaaaggct gtgttctcac
    15721 taaatacaca ggcgccagag gaaaaggcgg ctccaatgat gccagtgcag agaccatggc
    15781 gaaagttatc ggcattatgg aagatgcagg tgtttactgg caggcaggcg agcttggcgc
    15841 agtagatgca ggcggcggcg gcaccattgc caagtttgta gctcatatgg atgtggatac
    15901 agtggactta ggtgtgccga tcctttccat gcatgctcct tttgaacttg cttctaaatt
    15961 agacgtttac catacatata aagcatttaa ggcattttat gcgtcataaa acttattgta
    16021 agggcttttt tttcccgacg gtgtatggta taactatgta caacgcttaa agaaagtctg
    16081 tttttgtttt ccagggacgg acaaaaagtt cctgacagaa caaataagcg aaaagaaaga
    16141 aaagaaaagg acatgcaaca atgagaaaac tgaaattatt atgtttatgc ggtctgactg
    16201 cagctgttct gatgaccgga tgcgcaggca gcacagacac cacaaatgta aaccttggtt
    16261 ctgcaactgt ttctgagtac agaggagtaa agatcaatgt agcagctccg gaagtaacag
    16321 atgatgaagt agaccagaag atccagacca atctgaagca aaatccaaat gaagtagaag
    16381 tggacagagc tgcgcaggac ggcgatatcg tcaacatcga ctacaaggga acaaaagacg
    16441 gagaagcttt tgacggcggt accgcagaag gacaggatct gactcttggt tcaggatcta
    16501 tgatcgacgg ttttgaagac ggtctggtcg gagcaaagaa gggtgagaca aagaccttgg
    16561 atttaacttt cccggaaaat tacagagaag agtctttagc aggacaggca gttgtatttg
    16621 aagtaactgt aaatgctgta aaagaaaagc aggatgcagt attagatgat gcatttgtac
    16681 agagaacttc tgattaccag actgtagatg agtataagga aggcatccgt gctgacctgt
    16741 tagcccagaa gcagaagaca gcagagcagg aaatggagca ggatgttctt caggacatta
    16801 tcgataactc cgatttcaag ttctccagaa acggtttatc tgtacgttac aatcagatgt
    16861 taaagcagta taccaaccag gctaagatgt acggaatgac tctttcccag atggctcagg
    16921 ccaatggtat ggatgaagcc ggatttaagg aatacatcta ctcttctgta aaagaagcag
    16981 ctaagaaaga gatcgttgta aaagacattg cagcaaaaga aggtcttgat aacctgacag
    17041 atgaagataa agaagcattt gcacaggcaa atggaaccag caaagataca ctggtaagcc
    17101 tttatggaga agatactgta aatgagcagg tccttcagga taaggtatta agatttttag
    17161 cttccaatgc agacaatgaa gcagaaaatc ctgcaaaact gtctgagcgt gaagtaacgg
    17221 ttactgaaac ttcagcagac caggagtctt ccccagaaga aacaacagaa gctgaaacta
    17281 cagcagaaga aacaaaagca aactaaaata ttatattcaa aacaaatgga taaggaggat
    17341 attatacatg caactcttaa aagataggat ccgtaaggac ggcaagatca aagcgggaaa
    17401 tgtgctgaaa gtagacagtt ttttaaatca ccagatggat attaaactgt ttggtgagat
    17461 cggaaaggaa ttcaagcgca gatttgcgga tgtggaagta aacaagatcc ttaccatcga
    17521 ggcttccgga atcggaattg catgtattgt agcccagtat tttgatgttc ctgttgtatt
    17581 cgctaagaag acacagacca agaacattgc tggtgaagtc tatacttcta aagtagagtc
    17641 ctttacccac ggcagagtat atgacattat cgtatccaag gaattccttg gaaagggcga
    17701 caaagtgctt cttatcgatg atttcctggc aaacggaaag gcgttagaag gtctggcagc
    17761 tttagtaaga gattccggtg ctgaattagt aggtgctggt atcgtgacga aagagttgct
    17821 tactccgaaa gggttgcgta ctc
//
LOCUS       DBMDUA010000040        17382 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1903_length_17382_cov_2.208437, whole genome shotgun sequence.
ACCESSION   DBMDUA010000040 DBMDUA010000000
VERSION     DBMDUA010000040.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 17382)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17382)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17382
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1903_length_17382_cov_2.208437"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(55..1035)
                     /locus_tag="ACZPCL_04940"
     CDS             complement(55..1035)
                     /locus_tag="ACZPCL_04940"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR1161857.1"
                     /translation="MKDLKLWGIFISLIIVVQALGFFVFSGIAWCLFHAGILSPDHPP
                     MGWFPFLTNTCSSALISLCITTVISHHFFRPIQDLVHALEQVSAGNFDVRLKENDIWY
                     EVREMNLNFNKMVKELNSIETLQSDFIQNVSHEIKTPLAAIEGYAALLSASTRDEQNK
                     LYAEQILKSSRQLSTLTGNILKLSKLENQSIISEKKTFSLDEQIRQAVLSLEPIWSAK
                     NIDIDLDLPEISFYGNEDLIFQIWTNLISNGIKFTPSGGLVSVKMTAEDSFVNVVVAD
                     NGIGMTEEVQKHIFDKFYQAEGSRSMEGNGLGLTLVKNRLRLFNSPAPQS"
     gene            complement(1032..1634)
                     /locus_tag="ACZPCL_04945"
     CDS             complement(1032..1634)
                     /locus_tag="ACZPCL_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1161858.1"
                     /translation="MFKVLTVHNGQEAWDLIENEHVDLVITDILMPVMDGYEFTKLLR
                     HSNPGMPVLMITAKDDFPSKNKGFSLGTDDYMTKPVNLDEMVLRVRALLRRSHIASNK
                     RLTLPHTILDYDALTVTWGDDPQMLPQKEFFLLFKLISYPNKIFTRMQLMDEIWGRNS
                     ESDLQTIDVHINRLRRRFENNPDFEIITVRGLGYKAVMKE"
     assembly_gap    1692..1701
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2114..3271
                     /locus_tag="ACZPCL_04950"
     CDS             2114..3271
                     /locus_tag="ACZPCL_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR1161859.1"
                     /translation="MKKKIIIAGICIIALCAVIVPKFTKPKQFAQAVALPVMDAEKPE
                     TGDIRLTTSVIGKIEPSDVVYIYPKAAGDVTEVNVKAGDVVTKGQVLCNIDTKQVESA
                     KNTMDSAALTLRQAQEELSRQQLLYAGGGLSDQQYSQYQNAVSSAQINYDQAKYNYET
                     QLEYSQITAPMEGLVEICNVENYDNVSQGNLIFVISGQGSRVVSFSTTERIRGYLKEG
                     DTIDVEKDGQTYEGTVYEVSTMADETTGLYKVKASVDADVVLPTGSEVKLYVTSEKAE
                     GVMTIPVDAVYYENGEPYVYTYSDGKVWQKFIEEGIFDSDILEVKSGLSMDDMVVTTW
                     SSELYDGAEVRLKGETTQDDSTGDAAGAPAGGPEGNAPGGPGAPMTGNKAQ"
     gene            3285..6407
                     /locus_tag="ACZPCL_04955"
     CDS             3285..6407
                     /locus_tag="ACZPCL_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1161860.1"
                     /translation="MNGLTRFVLKRPITTLMAVLCLIVFGYTSVTSMSLENIPDMEMP
                     VLMVMTTYSGASPEDVNEIITKPIEDQVGTLSGLKSVTSNSSEGSSMVMLEYEYGTDT
                     DEAYDDLKKKVDLVKNSLPDSAGDPMIMEMDMNSMPCVTLSIENTSQENLYSYVEDKI
                     QPEFEKIAQVADVSVRGGSDEYIKIQIIPEKLSQYKMTMASVAADIAAADISYPSGDT
                     QVGSLKLSVSTRTTYDTVDLLKEIPLTSADGSTVYLEDVADVYTTTEENDGIARYNGN
                     DTVSLSITKQQSETAVSVSNAVKKTIANLEGADENLHIITVRDDADSIISSLKDVAQT
                     LVLAIIISMIIIWLFFGDLKASLIVGTSIPISILSALILMSLMGFTLNTITMSALTLG
                     VGMMVDNSIVVLESCFRMTAKRRGGFVEYMQDALEGTSLVWESVLGGTATTCVVFLPL
                     AFLQGMTGQMFKPLGFTIVFCMAASLISSITIVPLCYMAYKPTEKLSAPFSRPVKRLQ
                     EGYRKLMSTLLYKSKTVMLTSIALLVLALFIGKSLKMELMAADDEGEITVTVDTRPGL
                     QSKKVDEILKQVEAIISQDENLDSYMTSYGSSRRGGSSATVTGYLKDDRTMATADVAS
                     KWQQELSDITNCDITAEAGSSMSMMSATRQSYEVILKGTDYDEVKEVTNSVVEELKTR
                     DDVTKIHSDVENSSPVVEVRVDALKAKSAGLTPSQIATAVSNCIDGVKATTIQVNGED
                     IDVKVQYAEDEYKSIDQVRGIVLTTGKGGSVALSDVADVKFVDSPSSVARENKEYTVT
                     ISAEYTEKATQNTRNQIQEEVVKPHLTSTVSIGLNSRDQSRNEEFASLFKALAIAVFL
                     IFVVMAAQFESIKFSGMVMTTIPFSLIGAFGLLWLTDCSISMVSLIGFLMLIGTVVNN
                     GILFVDTANQYRGTMNRDTALIEAGATRIRPILMTTLTTVVSMIPMAFSGTTTKSLAI
                     VDIGGLTASTILCLLMLPVYYRLMSGREKPGDLEAPAKKRKRTRENIMNKIKGLFAGF
                     SKKGNKKDDQ"
     gene            6449..7645
                     /locus_tag="ACZPCL_04960"
     CDS             6449..7645
                     /locus_tag="ACZPCL_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR1161861.1"
                     /translation="MRKNKYGTYGTFSRSFSRVLAAAFVAAVAAGTMPVYSAYAASPS
                     FARTEEEWAKLRDNVIEYDELEGLIHEYNATVQNNQYTYQKFRQDYGDTNEEVAQEYY
                     KLAQDYYNDMSGDDDASSRMSDLNLQIQGDNMQKQGDETLEDSRIYLLTYEQAEKNLV
                     VTAQSDMISYYENLIQKEQAEATLTEAKENLALAQTKLSAGTITQLDLLTAQENVQTA
                     ENNLTQLENTIKNLKENFQVLLGWSYNDSPEIGSLTDVDENEIAAMNPDTDLAQAIEN
                     NYTLKINERKLENAKNETVKNQLTTAIANNKKQIGASLSGAYKKVLSAQISLQQAQTK
                     AQLEQTNLSNAALKMAAGMMTKNDYDAQERAAKTSENSVKTAQISLLSTVENYKWAVK
                     GLAAAQ"
     gene            7662..8999
                     /locus_tag="ACZPCL_04965"
     CDS             7662..8999
                     /locus_tag="ACZPCL_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014385.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR1161862.1"
                     /translation="MFLKDKYGKKWKLQAAKVLRTGALCVALAGVGTGVMGTLGELGA
                     DLGRLGLITAYADEFADRPEEVEGSSQIQVGNADGSDAEKEDTKGSDTAQWMGQVETD
                     ETKLQDNQLEYSELYTLISRYNPTIEQSLTSFNQSLEDYVNAWDELKFGQSSASTDLQ
                     TAKRDGDMENYAYYSQEKAVYKAASSMYKKMYDSMKKASDKSLRTMTRQLTVAAQSLM
                     MTYETLSLQKDTLTKQVEVYEKALNLEKTKQAAGMSTAADVLEKENQLLSARSSLSSL
                     EESLNSTYSSLCLMVGRDTDSGLVICEIPAADLAKADSMDLEADTKKAIGNNSSLISS
                     RGTKATSTAAKNNRNATVEEGEQNLTIQMNQLYENVLLKKSELQAAQTAFRKAEGQKK
                     NADIKYSAGLLSQEEYLTEEMNYISQTASYKAADLAFTQAADTYGWAVMGITQ"
     gene            9323..10141
                     /locus_tag="ACZPCL_04970"
     CDS             9323..10141
                     /locus_tag="ACZPCL_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR1161863.1"
                     /translation="MVRLILIGKRIYDKFSEDEMTVYAAQVSFFVILSFVPFLMLLLT
                     AVQMIPGISKADFLEIAMDIVPADYKSLAFRVVNDLMLKSPATMISATAVTALWSAGR
                     GMFSVARGLNRVNGRGRRHWYVFNRLICCGYTILFIAVCLMSMGLLVFGRTLQNFIQS
                     HFPLIAPFTGYVIQLRQLWGAAILLIFFLGIYCLVPDQKESVKVALPGAVFSTTGWML
                     FSLAFSLYFNFTGGKNYSYMYGSLAAIALSFLWLYICMCILFMGAELNWFWDEI"
     gene            complement(10243..11112)
                     /locus_tag="ACZPCL_04975"
     CDS             complement(10243..11112)
                     /locus_tag="ACZPCL_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1161864.1"
                     /translation="MNLKQMEYFVAIVNEGNISAAAKSLHISQPPLSAQMKLLEDELG
                     VVLFERGSRSIFLTEAGKVFYEKALHILHLTTAVSDELQQMGQGLKGPLHLGMISSVQ
                     TPRLIRALSSFVSLHPEVTLRISEGNTYELMDQLNSGLIEVAVVRTPLPTESYQCHFL
                     EAEPMMAVGKPEFFPDPAANSISVASLASCPLIVYRRWEPFISHCFPGTAPDYLCIND
                     DARTSMTWAQCGAGIALVPSSMAKAGGEDLLKIPLKDQGVMSRIALIARKHGMVSRVS
                     REFFQFFPNYFAN"
     gene            11263..13041
                     /locus_tag="ACZPCL_04980"
     CDS             11263..13041
                     /locus_tag="ACZPCL_04980"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1161865.1"
                     /translation="MKRLLSYMCAYKKESILGPLFKMLEASFELFVPLVVANMVDVGI
                     ANGSVSYVVRMGGLLLLLAIIGLTCSLTAQYFAAKAATGTATALRNNLFSHIGTLSYT
                     EIDTIGTSTLITRMTSDINQVQNGINMTLRLLLRSPFVVFGAMVMAFTVDAHTAMVFA
                     VTIPVLCVVIFGIMLISMPLYQSVQRQLDKVLLTTRENLMGVRVIRAFNRQENEREKF
                     EEENGSLVKMQVFVGKISALLNPVTYVIINIATVAVIWVGAGRVDAGIISQGKVIALV
                     NYMAQILVELIKMANLIILISKAVACMKRVDSVFEIKSSIEEKSHDTKTQNAENEMET
                     TQKISPETAKMEFDHVDFAYAGAKSDSLTDISFKAMKGQTIGVIGGTGSGKSTLVNLI
                     PRFYDVREGKVLIDGADVRDLPLTELRHAIGVVPQRAVLFKGTLRDNMRWGKEDATDE
                     EIWHALDVAQARDFIEAKGEGLDLMIDQGGHNLSGGQRQRLTIARALVRDPQILIMDD
                     SASALDFATDARLRKAIRENTKDMTVFIVSQRATTIRNADTILVLDDGKLAGIGNHKQ
                     LLKECDVYREICLSQLSKEEVERDEQ"
     gene            13031..14803
                     /locus_tag="ACZPCL_04985"
     CDS             13031..14803
                     /locus_tag="ACZPCL_04985"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008715979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1161866.1"
                     /translation="MSSKKPKMSKDKSIETLKRVLNYIAAYRWAVCLSLLLALVTVAF
                     TLYIPILTGKAVDEIVSQGNVDFSALISIIKKIIMAMAATAISQWLMNHINNIITYRV
                     VKDIRTKAFGHLEVLPLSYIDSHPSGDIISRIIADIDQFSEGLLMGFTQFFTGLMTIL
                     GTLLFMVSINPLITLVVVVLTPISLFVASFIAKKTYVMFKHQSETRGELTTLTDEMLG
                     NMKVVQAFGYQKEAEERFQTINNKLAGYSLRATFFSSITNPATRFVNSLVYAAVGITG
                     AYAAIRGFMTVGQLTSFLSYANQYTKPFNEISGVVTELQNALASAARVFELIDEKTIL
                     EDKKDATVLENVKGQVEIEHVNFSYTPDRPLIEDLNLSVKPGERVAIVGPTGCGKTTG
                     INLLMRFYDVNSGSIKVEGKDIRDVTRKSLRTSYGMVLQETWLKSGTIRDNIAYGRPE
                     ATEEEIINAAKEAHAHSFIMRMPQGYDTVISEDGGNLSQGQKQLLCIARVMLCLPPML
                     ILDEATSSIDTRTEIRVQKAFAKMMEGRTSFIVAHRLSTIREADMILVMRDGHIVEKG
                     KHEELLAKNGFYAEIYNSQFAAVG"
     gene            14954..16300
                     /locus_tag="ACZPCL_04990"
     CDS             14954..16300
                     /locus_tag="ACZPCL_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR1161867.1"
                     /translation="METDPQSASIFTQLLILLVLTLIRGYFAGAEMAVASVNKNKIHR
                     LKEQGNKKAALIERLMEGSTAFLSAIQLIVTLAGFLSSAVAATGISGILAVKMNRWGI
                     PGSKIAAVVVITLILVYVNLVFGELIPKRMALQNAEKFSLSCIGLVYFVSCIVKPFVG
                     LLNISTSGILKLFGMHHENLETDVSEEEIKSLLETGSETGVFNEIEKEMITSIFSFDD
                     KKAKEVMVPRQDMVAIDIDEPVDSYLDDILQSMHSKIPVYQEDIDNIIGILSTKALTI
                     EARKKSFENLDIRSLLTPAYFVPENRRTDALFREMQQKKEKLAILIDEYGGVSGMVTL
                     EDLIEVIVGDIHEEYEEVEPEIIELEPGHVYSLSGSIALSDLKEEFHLDMDSVCDTLS
                     GYLMEVLGYIPTEKALPLTVTTPEADYEILKMEDRVIDRVKMTLKEAQTSETGDAT"
     gene            16455..17219
                     /locus_tag="ACZPCL_04995"
     CDS             16455..17219
                     /locus_tag="ACZPCL_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1161868.1"
                     /translation="METKNVIFELRTKKGLSQEELAQKVMVTRQAVSRWENGETVPNT
                     ETLKLLSKEFDVSINTLLGDPRQLVCQCCGMPLEDGIISHNGDGTLNEEYCKWCYADG
                     TYTYSNMDDLIDVCVKHMVNDKFTEEQARSYMKQLLPSLDYWKRYEELSDNGQFEAFK
                     EQLIKEINDLHIEGLPKVEKLNALVGSYVNLGYPLPNGKTVKFLNDEITYLGNQLESE
                     FGGDRCFGVLANMDFIMISTYGPEGETPELVLYKKR"
     gene            17234..17382
                     /locus_tag="ACZPCL_05000"
                     /pseudo
     CDS             17234..17382
                     /locus_tag="ACZPCL_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392127.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT         5166 a         3535 c         4181 g         4490 t
ORIGIN      
        1 atattcataa gactattatg agatagattt tagaaaaaag aaacccctaa cccctcaaga
       61 ttgaggggca ggggagttga ataggcgaag cctatttttt actaatgtca gtccaaggcc
      121 attgccttcc atgctgcggc ttccttctgc ctggtaaaat ttgtcaaaaa tatgtttttg
      181 cacctcttcg gtcataccaa tgccgttgtc cgcaacgacc acatttacaa aagagtcttc
      241 tgcagtcatt tttacagata caagaccgcc ggatggagta aacttaattc cattggagat
      301 cagattggtc cagatctgaa agatcaggtc ttcgtttcca tagaaagata tttccggaag
      361 atccaggtca atatcaatat ttttggcaga ccagatgggc tctagggaaa ggactgcctg
      421 acggatctgt tcatctaaag agaaggtttt cttttcagag ataatggact ggttttccag
      481 tttggacaat tttaaaatgt ttccggtaag agtagaaagc tgccgggagc tttttaagat
      541 ctgttctgcg tacagcttat tctgttcatc cctggtagaa gcagacagaa gggcagcgta
      601 gccttcaatg gcagcaagag gggtttttat ctcatgggat acattctgga taaaatcaga
      661 ctgcaaagtt tcaatggaat ttaactcctt caccatttta ttgaagttta aattcatctc
      721 cctgacttca taccagatgt cattttcttt taaccgcaca tcaaaatttc ctgcagatac
      781 ctgctctaaa gcatggacca ggtcctggat agggcggaaa aagtggtggg agatgactgt
      841 ggtaatgcac agggagatca gtgcggaact gcaggtgttg gtgagaaaag gaaaccagcc
      901 cataggggga tgatccggac ttaatatgcc tgcgtggaat aaacaccagg caatgccgga
      961 aaatacgaaa aatcccagtg cctgcacaac aatgatcaaa ctgataaaaa taccccataa
     1021 tttcagatct ttcattcttt catcaccgcc ttatatccaa gtccacggac tgttatgatc
     1081 tcaaaatccg gattattctc aaaacgtctg cgcagccggt tgatatgtac atcaatggtc
     1141 tgtaggtctg actcactgtt gcgcccccag atctcatcca ttaactgcat acgggtaaag
     1201 atcttatttg gataggagat cagcttaaat agcaggaaaa attccttctg gggaagcatc
     1261 tggggatcat ctccccaggt tacggtcagt gcatcataat ccaagatagt atgaggcaga
     1321 gtaagccgtt tattactggc aatgtgtgaa cggcgaagca gtgcccgcac cctcaatacc
     1381 atttcatcta aatttaccgg ttttgtcata taatcgtctg ttcccaggga aaagccctta
     1441 tttttagaag gaaaatcatc ttttgcagtg atcatcagta ccggcatgcc ggggttggaa
     1501 tggcgcagca gtttggtaaa ttcatagccg tccataacag gcattaaaat atcggtgatc
     1561 accagatcta catgttcgtt ctcgatcaga tcccaggctt cctgtccatt gtgtacagtt
     1621 aaaaccttaa aaatgccatt ctttcccttc ctatttcaaa ggccttttct gcagctacca
     1681 ttcttgcttc annnnnnnnn ntacagttaa aaccttaaaa ccttcttttt ccagcacaat
     1741 gcggtagagc tgcagcaggg agtcttcgtc ttctattaca agaatggtca atatctgcat
     1801 ggtagtttct ttgcctttct tctttatata ttttatagat tgatcctatt ttgggcctgc
     1861 atcttcagat cagcctcatt atcatattca gcatagcaca ttttcatgaa catcaggtga
     1921 aatattggga acaaaataaa agaataagga aagaaagttc aaaaagacct gaatggaaat
     1981 gtgaaaaata tgtgatttca tcttcagttc atcttcgatt aagaaagggt tcattaagta
     2041 atggtatcct taggaagaat gttaatgaga aaagcagcgt tttttgctgc gtggggaaag
     2101 gataagagaa aagatgaaga aaaagatcat aattgcaggg atctgtatta ttgcattgtg
     2161 tgcggtcatc gtacctaaat ttacgaaacc gaaacagttt gcccaggcag tggctcttcc
     2221 tgttatggat gcagaaaaac ctgagacagg agatatccgg ctgaccacca gtgtgatcgg
     2281 taagatcgag ccttctgatg tggtttatat ttatccaaaa gcagccggtg atgtaacaga
     2341 ggtaaatgta aaggccggtg atgtggtgac caagggacag gtcctttgca acattgacac
     2401 aaaacaggta gagtcagcaa aaaataccat ggactcagct gcattaacac tgcgccaggc
     2461 tcaggaagaa ctttcccgcc agcagctttt atatgcagga ggcggacttt ctgaccagca
     2521 gtacagccag tatcagaatg cagtgtccag tgcccagata aactatgacc aggcaaaata
     2581 taactatgaa acacagctgg aatacagcca gatcacagca cctatggaag gtctggtaga
     2641 gatctgtaat gtggaaaatt atgacaatgt atcccagggc aatctgatct ttgttatttc
     2701 cggacaggga agcagagtgg tatctttctc cactacagag cgcatcagag gctatctgaa
     2761 agaaggagat accattgatg tggaaaagga cggacagact tatgaaggta cggtttatga
     2821 agtaagtacc atggcggatg agactacagg actttacaag gtaaaggctt ctgtggacgc
     2881 agatgtggtt cttcctaccg gttctgaagt aaagctgtat gtaacttctg aaaaggcaga
     2941 gggagttatg accattccgg tagatgctgt ttattatgaa aatggcgaac cttatgtgta
     3001 tacatattca gacggaaaag tatggcagaa atttattgaa gagggaattt ttgactccga
     3061 tattttagag gtaaaatccg gtctttctat ggatgacatg gtagttacta cctggagctc
     3121 tgagctgtat gacggagcag aggtaagatt aaaaggggaa acgacacagg atgacagcac
     3181 cggtgatgca gccggagcac ctgcaggtgg accagaaggg aatgcaccag gcggaccggg
     3241 agctcccatg acaggaaaca aggcacagta aggaggacga cataatgaat ggtttaacaa
     3301 gatttgtcct caaacggccc attaccacat tgatggcagt gctctgcctg atcgttttcg
     3361 gctacacttc tgtaaccagc atgagcctgg aaaatatccc ggatatggaa atgccggtac
     3421 tgatggttat gaccacctat tccggagcaa gccctgagga cgtaaatgag atcattacaa
     3481 agcctattga ggatcaggta ggaactttaa gcggcctgaa atctgttaca tctaattcca
     3541 gtgaaggcag ttccatggtt atgctggaat acgaatacgg tactgataca gatgaggcat
     3601 atgatgatct gaaaaagaag gtagatctgg taaagaacag tcttcctgac agtgcaggcg
     3661 atcctatgat catggaaatg gatatgaact ccatgccctg tgttacctta tccattgaaa
     3721 atacctccca ggaaaacctg tattcctatg tagaagacaa gatccagccg gagtttgaaa
     3781 agatcgccca ggtagcagat gtaagtgtcc gtggcggttc ggatgaatat attaagatcc
     3841 agatcattcc ggaaaaatta agccagtata aaatgaccat ggcttctgtt gcagcggata
     3901 ttgcagcagc agatatttcc tatccttctg gtgataccca ggtgggaagc ttaaagcttt
     3961 ctgtttccac ccgtaccact tatgataccg tagatctgtt aaaagagatc ccactgacca
     4021 gtgcagacgg aagcaccgtt tacctggaag atgtggcaga tgtatacact acaacagagg
     4081 aaaatgacgg tatcgcccgt tacaatggaa atgatacagt gtcgctttcc attacaaagc
     4141 agcagagtga aacagcagtt tctgtttcca atgcagtaaa aaagacaatt gcaaatctgg
     4201 aaggagccga tgaaaactta catatcatca cagtacgtga tgatgcagac agtattataa
     4261 gctctttaaa ggatgtagcc cagacactgg tcctggcgat cattatctcc atgatcatta
     4321 tctggctgtt ctttggtgac ttaaaggcgt ccctgatcgt aggtacttcc attcctattt
     4381 ccattttgtc agcactgatc ttaatgagtt taatgggctt taccttaaat accattacca
     4441 tgagtgcgct gacacttggt gttggtatga tggtagataa ctctatcgtt gttctggaaa
     4501 gctgtttccg tatgacagca aagcggcggg gcggatttgt agaatatatg caggatgcct
     4561 tagaaggtac cagtctggta tgggagtccg tactcggcgg tacagccact acctgtgttg
     4621 tattcctgcc actggcattt ttacagggta tgaccggaca gatgtttaag ccattgggct
     4681 ttacaattgt attctgtatg gcagcatccc tgatctcatc cattaccatt gtgcccctgt
     4741 gctatatggc ctataagcca acagaaaagc tttcagcgcc attttccaga cctgtaaaga
     4801 ggctgcagga aggatacaga aagctgatga gtacccttct ttataagtca aagacagtta
     4861 tgcttacttc cattgccctt ctggttttag cccttttcat tggaaaatcc ctgaaaatgg
     4921 agctgatggc tgcagatgat gaaggtgaga tcacagtaac tgtagataca agaccaggac
     4981 ttcagtcgaa aaaggtagat gagatattaa agcaggtaga agccattatc agtcaggatg
     5041 aaaacctgga ttcttatatg acatcctatg gaagttccag gagaggcggc agttctgcta
     5101 cagttacagg ctatctgaag gacgaccgta ccatggcaac agcagatgtg gcatctaaat
     5161 ggcagcagga gctttctgat attacaaact gcgatattac cgcagaggcc ggttcttcca
     5221 tgagcatgat gtctgctacc agacagagct atgaggtgat cttaaaggga acggattatg
     5281 acgaagtaaa agaagtaacc aattccgttg tagaagagtt aaaaacaagg gatgatgtga
     5341 caaagatcca ttctgacgtg gaaaactcat ctcctgtagt agaagtaaga gttgatgcct
     5401 taaaggcaaa gtcagcaggt cttaccccat cccagatcgc gactgcagta agcaattgta
     5461 ttgacggtgt aaaggcaacc accatccagg taaacggtga agatattgat gtaaaagtac
     5521 agtatgcaga ggacgaatat aagagcattg accaggtacg tggcattgtt cttaccaccg
     5581 gaaaaggcgg ttctgtggct ttgtctgatg tggcagatgt aaaattcgta gacagccctt
     5641 cttctgtagc aagagaaaat aaggaatata cagtgaccat ctctgcagaa tatacagaaa
     5701 aggccaccca gaataccaga aaccagatcc aggaggaagt agtaaagcct catctgacca
     5761 gcacagtaag tattggctta aattccaggg atcagtccag gaacgaggag tttgcatccc
     5821 tgtttaaggc acttgctatt gcagtattct tgatctttgt tgtaatggca gcccagtttg
     5881 agtctattaa gttctccgga atggtcatga ccactatccc gttcagtctg atcggtgcat
     5941 ttgggctgtt gtggctcact gactgttcca tcagcatggt ttccctcatc ggtttcctga
     6001 tgctgattgg tactgttgta aataatggta ttttgttcgt ggatactgca aaccagtacc
     6061 ggggaaccat gaatcgtgat acagctctta ttgaggcagg cgctacccgt atccgaccta
     6121 ttctgatgac aacgctgaca accgttgttt ccatgatccc tatggcattt agcggaacca
     6181 ctacaaagag tcttgctatt gtagatattg gtggtctgac agcttccacc atcctgtgcc
     6241 ttttgatgct tcctgtatat taccgcctca tgagcggcag ggaaaaacca ggagaccttg
     6301 aggcaccggc aaagaagcgt aaacgtacca gagagaatat tatgaacaaa ataaaaggat
     6361 tatttgcagg atttagcaaa aaagggaaca aaaaggatga tcagtaataa agatagcaat
     6421 ggatcaaagg atagaaagaa ggatgaccat gaggaaaaat aaatatggta catatggtac
     6481 attttccaga tcattcagcc gtgtgctggc agccgctttt gtggcggctg tggcagcggg
     6541 gactatgcct gtatacagtg cctatgcagc aagtccctct tttgccagga ccgaagagga
     6601 atgggcaaaa ttaagggata atgtgatcga atatgatgaa ctggaagggc tgatccatga
     6661 gtacaacgct acagtgcaga ataatcagta tacttaccag aaattccgcc aggactatgg
     6721 ggataccaat gaggaagtgg cccaggaata ttataagctg gcccaggact attacaacga
     6781 tatgtccggg gatgatgatg cttcttcccg tatgtcggac ctgaatttac agatccaggg
     6841 agacaatatg caaaagcagg gagatgaaac tttggaggac agtaggattt atcttcttac
     6901 ctatgagcag gcagaaaaga acctggttgt tacggcccag tcagatatga tctcttatta
     6961 tgagaatttg atccagaagg agcaggcaga ggcaaccctt acagaggcaa aggaaaatct
     7021 ggctctggca cagacaaagc tttctgcagg taccattacc cagttagatc tgctgacagc
     7081 ccaggaaaat gtacagacag cagaaaataa cttgacccag ctggaaaata cgatcaagaa
     7141 cttaaaagag aatttccagg tgcttttagg ctggagctac aatgactcac cggagatcgg
     7201 aagtctgacg gatgtggatg aaaatgagat cgcggccatg aacccggata cagacctggc
     7261 acaggcaatc gagaacaact ataccttaaa gatcaatgag cggaagctgg aaaacgcaaa
     7321 aaatgagaca gtaaagaacc agctgactac agccattgcc aataataaga agcagatcgg
     7381 cgcatcttta tccggcgctt acaaaaaggt gctttcagcc cagatctctt tacagcaggc
     7441 ccagacaaaa gcacagttgg agcagaccaa tctttccaat gcagctttaa agatggcagc
     7501 aggaatgatg actaaaaatg attacgatgc ccaagaacgg gctgcgaaaa ccagtgaaaa
     7561 ctcagtaaaa acagcacaga tcagtctgtt aagcacagtg gaaaattata aatgggcggt
     7621 aaaaggcctg gcagcagccc agtaggaagg aggcagcttg tatgttttta aaggataaat
     7681 atggaaaaaa atggaaactg caggcggcaa aggttttaag gacaggtgcg ctttgtgtgg
     7741 cgttagctgg tgtcggtaca ggcgttatgg gcactttggg agaacttgga gctgatctgg
     7801 gccggctggg gctgatcact gcctatgcag atgaatttgc agaccggccg gaagaagtgg
     7861 aaggcagctc ccagatccag gtgggaaatg cagacggctc ggatgcggaa aaagaggata
     7921 ccaaaggttc tgatactgcc cagtggatgg ggcaggtgga gaccgatgag acaaagctgc
     7981 aggataacca gttagaatac agtgagctgt atacactgat ctcacggtac aatcctacta
     8041 ttgagcagtc tttaaccagt tttaaccagt ctttggaaga ttatgtcaat gcatgggatg
     8101 aattgaagtt tggacagtca agcgcgtcta cggatctgca aactgctaaa agggatggcg
     8161 atatggaaaa ttatgcttac tacagccagg aaaaagcagt ttacaaagct gcatccagca
     8221 tgtataaaaa aatgtatgac agcatgaaaa aagccagtga taaaagttta aggaccatga
     8281 cccgccagct gacagtggca gcccagtccc tgatgatgac ttatgagact ttaagtcttc
     8341 agaaggatac actgacaaaa caggtggaag tatatgaaaa ggctttaaac ctggaaaaaa
     8401 caaaacaggc agcaggaatg tctacagcag ctgatgtgct ggaaaaagaa aatcagttgt
     8461 taagtgctag gagcagcctt tcttccctgg aagagagctt aaacagcact tacagcagtc
     8521 tctgccttat ggtaggacgg gatacggaca gcggccttgt gatctgtgag atcccggcag
     8581 cagaccttgc aaaggcagac agtatggatc tggaagcgga tacgaaaaag gctataggaa
     8641 ataacagctc cctgatcagc agccgtggca cgaaggcaac ttctacagca gcaaaaaata
     8701 accggaatgc taccgtagaa gagggggagc agaacctgac tattcagatg aaccagcttt
     8761 atgagaatgt attgctgaaa aaaagtgagc ttcaggcagc tcagactgcg ttccggaagg
     8821 cagaaggaca gaagaagaat gcggatatta aatacagcgc cggtttatta agccaggaag
     8881 aatatctcac agaagaaatg aactatattt cccagacagc aagctacaag gcagctgacc
     8941 tggcatttac ccaggcggct gatacttatg gatgggcagt tatgggaatt acacagtagg
     9001 atttaaggat aagaatttag gatataagat cgggatacag gcagcgccgg agagggcgtt
     9061 gaacttgaag gatatttatg ttaaaatgca ggcatgtgta ttgtgatgcc tgcatttttt
     9121 ggagtaaaag ccggtattcc cggcagcgcg gggagcagcc agccggacat agccagatgt
     9181 ccataaaatc cccatgttcc cccgcgacgc ggggggaaca gtcactcaga ctttgacatg
     9241 ggcgcaatac aactgttgtt gacacatctc cgccccgcag ggggcggtga tccactttgc
     9301 tttaataagg aggacttgac ccatggtacg tctcattctc attggaaaac gtatctacga
     9361 taaattttca gaagatgaga tgacggtgta tgcggcccag gtctcttttt ttgtgatcct
     9421 ttcttttgtt ccctttctga tgctgcttct cacagcggtc cagatgatcc cgggaatttc
     9481 caaggcggat tttttagaga tcgccatgga tattgtaccg gctgattata aatctctggc
     9541 atttcgtgta gtcaatgacc tgatgttaaa atccccggca accatgatct ctgctacagc
     9601 tgtaaccgct ctctggtctg caggaagggg tatgttcagc gtggcaagag gcttaaaccg
     9661 ggtcaatgga agaggaagaa ggcactggta tgtgtttaac cgcctgatct gctgcggtta
     9721 taccattctc tttattgctg tctgccttat gtccatgggc cttctggtat ttggacggac
     9781 gctccagaat tttatccaga gccattttcc tcttatcgcc ccatttaccg gctatgtgat
     9841 ccagctgcgc cagctgtggg gcgcagctat ccttcttatt ttctttttag ggatctactg
     9901 tctggtgccg gaccagaagg agagcgtaaa agtagcgttg ccgggagctg tattttccac
     9961 aaccggctgg atgctgtttt ctcttgcatt ttccctgtat tttaatttta caggagggaa
    10021 aaactattct tatatgtacg gcagccttgc agccattgct ttgtcatttt tgtggcttta
    10081 tatctgtatg tgcatattgt ttatgggagc ggaacttaac tggttctggg atgagatata
    10141 agtagtggct cctttgctca aggaattgct acgtgctttg cactttcgca tctctgctcc
    10201 gcttcggtcg gtgctgaaca tgttccactg gcacatagca tcctagttcg caaagtagtt
    10261 tggaaaaaac tggaaaaatt ctctggatac ccggctgacc ataccgtgtt ttctggcgat
    10321 cagcgcgatc cggctcatga ccccctgatc tttcaggggg atttttaata agtcttcgcc
    10381 ccctgctttt gccatggaag aaggcacaag ggcaattccg gcgccgcact gggcccaggt
    10441 catgctggtc ctggcatcgt cattgataca aaggtaatcc ggagcagtgc caggaaagca
    10501 gtggctgata aaaggttccc atctgcggta gacaataaga ggacaggaag ccagggaagc
    10561 taccgatatg gaatttgctg caggatccgg gaaaaattcc ggctttccta ctgccatcat
    10621 aggttctgcc tctaaaaagt ggcactgata agactctgta ggaagtggag tccttaccac
    10681 tgcaacttca atgagaccgg aatttagctg atccatcagc tcatatgtat ttccctcact
    10741 gatgcgaagg gtcacctccg gatgaagact gacaaaagag gagagtgccc ggatcagcct
    10801 gggagtctgg actgaagaga tcattccaag atgcagaggt ccttttaatc cctgacccat
    10861 ctgctgcagt tcatcagaaa cagccgtagt cagatgtaag atgtgcaaag ctttttcgta
    10921 gaaaaccttt cctgcctctg tgagaaaaat actcctggag ccccgctcaa aaagcaccac
    10981 gcccagttcg tcttccaaca gtttcatctg ggcgcttaaa ggaggctggg agatatgcag
    11041 agatttggca gcggcagaga tatttccctc atttacaatg gctacaaaat attccatctg
    11101 tttcaggttc ataaaagtac ctcttttcca aaaaaaatat agcaatacct atacgataaa
    11161 catataactt ccatattaaa tcaatatttt aaatatatat ttttatgtgc tataatacca
    11221 gatagttaaa taaaatacaa tatttcatag gagaaatgca ttatgaagcg attgctcagt
    11281 tacatgtgtg cctacaaaaa agagagcata ctgggaccgc tgtttaaaat gctggaggcc
    11341 agctttgagc tgtttgtgcc tctggtagtg gccaatatgg tagacgtagg tattgcaaac
    11401 ggaagtgtat cttatgtggt gcgtatggga ggtcttcttt tgctccttgc aatcattgga
    11461 ctgacctgtt ccctgacagc ccagtacttt gcagccaagg cagcaacagg aacagccacc
    11521 gcactgcgaa ataacctgtt ttcccatatt ggtaccttat cttatacaga gatcgatacc
    11581 ataggaacct ccaccctgat cacacgaatg accagcgaca tcaaccaggt gcagaatggt
    11641 atcaacatga ccctgcgtct gctgttacgt tccccattcg ttgtattcgg tgccatggtc
    11701 atggcattta cagtagatgc ccatacagcg atggtatttg cagtgaccat tcctgtactc
    11761 tgcgtggtaa tatttggtat catgttgata agtatgccat tataccagtc tgtacagaga
    11821 cagctggata aagttctctt gactacgaga gaaaatctca tgggtgtgcg cgtgatccgc
    11881 gcttttaacc gccaggaaaa tgagagagag aagtttgaag aagaaaacgg cagcctggta
    11941 aagatgcagg tatttgttgg aaagatctcc gcactgttaa atcctgttac ttatgtgatc
    12001 attaatattg ctaccgtagc tgtgatctgg gtaggtgccg gaagagtaga tgccggtatc
    12061 atcagtcagg gtaaagtcat tgcccttgta aactatatgg cccagatcct ggttgaactg
    12121 atcaagatgg caaacctgat catcctgatc tccaaggcag ttgcctgcat gaagcgtgtg
    12181 gacagtgtat ttgagatcaa gagcagtatt gaggaaaaat ctcatgatac aaaaactcaa
    12241 aatgctgaaa acgaaatgga aactacacag aagatcagcc cagaaactgc aaaaatggaa
    12301 tttgaccatg tggatttcgc ctatgcagga gcaaaatctg actcccttac agacatttcc
    12361 tttaaggcaa tgaagggaca gaccattggt gtcatcggtg gtacaggttc aggtaagtcc
    12421 acattggtaa acctgatccc aagattttat gatgtaagag aaggaaaggt tcttattgac
    12481 ggagcagatg taagagatct gcctcttaca gaattaagac atgccatcgg tgtagtacct
    12541 cagagagctg tcctctttaa gggaacgctc cgtgacaata tgcgctgggg caaggaagat
    12601 gctaccgatg aagagatctg gcatgcactg gatgttgccc aggcaagaga ttttattgaa
    12661 gccaagggcg aaggtcttga tctgatgatc gaccagggcg gccataactt atctggtgga
    12721 cagcgtcagc gtcttaccat tgcaagagca ctggtacggg atccgcagat cctgatcatg
    12781 gacgacagtg cttctgctct tgactttgca accgatgcaa gactgagaaa ggccatccgg
    12841 gaaaatacaa aggatatgac tgtatttatc gtatcccaga gagctaccac catccgcaat
    12901 gcagatacca ttcttgtact ggatgatgga aaactggcag gtattggaaa tcacaaacag
    12961 cttctgaagg aatgcgatgt ttacagggag atctgcctgt cacagctgtc aaaagaggag
    13021 gtggaacggg atgagcagta aaaagccaaa aatgagcaag gataagagca ttgaaacatt
    13081 aaaaagagtc cttaattaca ttgccgctta cagatgggcc gtatgtttat cccttctgtt
    13141 agcattagta acagttgcat ttacccttta tatcccaatt cttacaggta aagcagtaga
    13201 tgagatcgta agtcagggaa atgtagattt ctcggcactg atcagtatta ttaaaaagat
    13261 catcatggca atggcagcta ccgccatcag ccagtggctt atgaaccata ttaacaacat
    13321 tatcacctac agagttgtaa aagatatccg taccaaagct ttcggacatc tggaagtcct
    13381 tcctttaagt tatatcgact cccacccgtc aggtgatatc atcagccgta ttattgcaga
    13441 tatcgaccag ttctccgaag gtctgcttat gggatttacc cagtttttta caggactgat
    13501 gactatttta ggaaccctgc ttttcatggt ttccattaac ccgctgatca cactggtagt
    13561 tgtagtttta acacctatct ctctgtttgt ggcaagtttc attgcaaaaa agacttatgt
    13621 tatgtttaaa catcagtctg aaaccagagg tgagctgacc acactgaccg atgaaatgct
    13681 gggaaatatg aaggtagtcc aggcctttgg ctatcagaaa gaagcagagg agcgtttcca
    13741 gacgatcaat aacaagctgg ctggctacag tctgcgcgct accttcttct catccattac
    13801 caatccggct acccgttttg taaacagcct ggtttatgct gcagttggta ttacaggcgc
    13861 ttatgcagct atccgtggtt ttatgactgt tggacagctt accagtttct taagctatgc
    13921 aaaccagtat acaaagccgt ttaacgagat ctcaggcgtt gtaacagagc ttcagaacgc
    13981 ccttgcatct gcagccagag tatttgagct gatcgatgaa aagaccattc tggaagataa
    14041 gaaagacgcc acagtccttg aaaatgtaaa gggccaggtg gagatcgagc atgtaaactt
    14101 ctcctataca ccagacagac cgctgatcga agatctgaac ctttctgtaa aacctggtga
    14161 gagagtggcc attgtaggac ctaccggctg tggaaagact accgggatca acctgcttat
    14221 gcgtttttat gatgtaaatt ccggttctat taaagtagaa ggaaaggaca tccgcgatgt
    14281 gaccagaaag agccttcgta ccagctatgg tatggtgctt caggagacct ggttaaaatc
    14341 cggtacgatc cgggacaata tcgcctacgg aagaccagaa gcaacagaag aagagatcat
    14401 aaatgcagct aaagaggcac atgcacacag ctttatcatg cgaatgcctc agggttatga
    14461 cacagtgatc agtgaggacg gcggaaacct gtcccagggc cagaagcagc ttttgtgtat
    14521 tgccagagtt atgctctgcc tgccgccaat gctgatcctg gatgaggcaa cttcctctat
    14581 cgatacccgt acagagatcc gcgttcagaa ggcctttgcc aagatgatgg aaggaaggac
    14641 cagctttatt gtagcccacc gtctttctac gatccgtgag gcagatatga tccttgttat
    14701 gcgtgacgga catattgtag aaaaaggaaa gcatgaagaa cttcttgcaa agaacggctt
    14761 ttatgctgaa atttacaaca gccagtttgc tgctgtagga taaaagaaat attaaaagta
    14821 aaaaagtcag tccctttttg agaaaatagg ggctgacttt tttcgtgact gtctgtataa
    14881 taaagtctaa atgataatca tacggatgat aatttgcgaa aatgcataca tccaaaagga
    14941 ggaatttttt attatggaaa ccgatccgca atcggccagt attttcacac agcttcttat
    15001 cttgctggta cttactctta taagagggta ttttgcagga gcagagatgg ctgtggcttc
    15061 tgtaaacaaa aacaagatcc acaggttaaa agaacaggga aataagaaag cagccctgat
    15121 cgagcgcctg atggaaggat caacagcatt tttatctgcg atccagttaa tagtcacact
    15181 ggccggtttt ctttccagcg ctgtggcagc aacgggaatt tcaggaatac tggctgtaaa
    15241 gatgaataga tggggaatac caggcagtaa gattgctgca gtggtggtca ttacccttat
    15301 tcttgtttat gtgaatctgg tattcgggga actgatcccc aaacgaatgg cattgcagaa
    15361 tgcggaaaaa ttcagtttat cctgcattgg cctggtttat tttgtatcct gtatcgtaaa
    15421 gccttttgta ggccttttaa atatttctac cagcgggatc ttaaagcttt tcggtatgca
    15481 ccatgaaaat ctggaaacag atgtatctga ggaggagatt aaatcccttc tggaaacagg
    15541 aagtgagacc ggcgtattta atgagatcga gaaagagatg atcacttcta ttttctcatt
    15601 tgatgataaa aaagcaaaag aagtcatggt accgagacag gatatggtag ccattgacat
    15661 tgacgaaccg gtggactctt atctggacga tattttacag tccatgcatt ccaagatccc
    15721 ggtatatcag gaagatattg ataatatcat cggtatcctt tccacaaagg cattgaccat
    15781 tgaggccaga aagaagtcct ttgaaaatct ggatatccgt tcccttttaa cacctgctta
    15841 tttcgttccg gaaaacaggc gtacagacgc ccttttcagg gaaatgcagc agaaaaaaga
    15901 aaaactggct atactgatcg atgaatacgg cggcgtatcc ggtatggtca ctctggaaga
    15961 cctgatcgag gtgatcgtag gtgatatcca cgaagaatac gaagaagtag aaccggagat
    16021 catcgaattg gagccgggac atgtatacag cctttccgga agtattgcac tgtcagattt
    16081 aaaagaggaa ttccacctgg atatggacag cgtatgtgat accttgtccg gttatctcat
    16141 ggaagttctg ggatatatcc ctactgaaaa ggcgctgccc cttacagtga ccaccccaga
    16201 agcagattac gagatcttaa aaatggaaga ccgggtgatc gaccgggtaa aaatgacact
    16261 gaaagaagcg cagacctcag aaaccgggga tgcaacataa aaatctaagc agtcctgctt
    16321 tacaatgggg actgcttttt tgctgccata cattcggaaa aggctgccag cggctttcaa
    16381 tatctataga aacagcaagt tgctttccca aagcctgtaa ttctgttatg atctatataa
    16441 cggaggtaga taccatggaa acaaaaaatg ttatttttga gcttcgcacg aaaaaagggc
    16501 tgtctcagga agagttagca caaaaggtta tggttacacg gcaggcggtg tcacgctggg
    16561 aaaatgggga gaccgttccc aatacggaga ccttaaagtt gttatcgaag gaatttgacg
    16621 tgtccatcaa taccctttta ggcgacccaa ggcagcttgt gtgtcagtgc tgcggaatgc
    16681 cattagagga tgggattata agccataacg gtgatggcac attgaatgaa gagtattgta
    16741 aatggtgcta tgccgatgga acctacacct atagtaatat ggatgatctt attgatgtct
    16801 gcgtaaaaca tatggtaaat gataaattca cagaagaaca ggcgcgttct tatatgaagc
    16861 aactgctccc cagtcttgat tattggaaac ggtatgaaga attaagtgat aatggacaat
    16921 ttgaggcgtt taaagaacaa ttgataaaag agatcaatga tcttcacatt gaaggattac
    16981 cgaaagtgga aaaactgaat gcccttgtgg gcagctatgt aaatctggga tatccgctgc
    17041 caaacggaaa aacagtaaaa tttttaaatg atgagatcac ctatctgggc aatcagctgg
    17101 agtcagaatt tggcggtgac aggtgtttcg gcgtccttgc caatatggat ttcattatga
    17161 tcagcacata cgggccagaa ggggaaactc cggaactggt tctttataag aaaagataac
    17221 aaatctattg ctgtttgatg gattgaaaga tgcagtagtc cgtatgcttg caggagaaag
    17281 agttcaggtc aatacgggaa cattttccaa tgatatgaca aactttcagg gaaaagatga
    17341 tgtgctgaca ttactgatcc atctgggata tctcagctat ca
//
LOCUS       DBMDUA010000041        17375 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1907_length_17375_cov_2.706669, whole genome shotgun sequence.
ACCESSION   DBMDUA010000041 DBMDUA010000000
VERSION     DBMDUA010000041.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 17375)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17375)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17375
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1907_length_17375_cov_2.706669"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            10..2271
                     /locus_tag="ACZPCL_05005"
     CDS             10..2271
                     /locus_tag="ACZPCL_05005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007038577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR1161869.1"
                     /translation="MANALLDQDVPVYTDDLALKDCLIVKVLRSPHAHALIEEIDCKV
                     ASRVPGIECILTWKDVPQKRFTMAGQTYPEPSPYDRLILDQRVRFVGDAVAIIAGETE
                     AAVDHAMRLIKVKYQVLEPVLDMHDSKDGKILVHPEDSWKALCNVGADNKRNLCASGG
                     ETHGDLEAAFAGCSHIVEKTYHTKANQQAMMETFRAFTYMDVYGRLNVVDSTQVTFHV
                     RRILAHALDVPKSKIRVIKPRIGGGFGAKQTVVAEVYPAIVTMKTGKPAKIIYSRYES
                     QIASSPRHEMEVKVKIGCDDNGILQAMDVYTLSNTGAFGEHGPTTVGLSGHKSIPMYG
                     TPKAFRFAYDVVYTNRMSAGAYRGYGATQGIFAVESAMDELAAQMGMNPIELRKKNMV
                     RQGQVMPAYYGETANSCALDRCVDKVMEMIGWDEKYPRKDMGNGKVRAVGLAMAMQGS
                     AISGVDVGSVTIKVNDDGFYSMTIGAADMGTGCDTTLAQVAAECLDCELDDIVVYGVD
                     TDISPYDSGSYASSTAYLTGMAVVKTCESLKKKILKKAAEYLNCSEDELEFAGKTVRR
                     LNSPEGEPAEITLKDIGNRAMCFNEEALQATESHSSPVSPPPFMAGAAEVEIDKETGH
                     IELLQFAAAVDCGTPLNENLARVQTEGGLAQGIGMAMYEDVTYSDHGRVHENSFMQYK
                     VPSRLDIGKVQVEFECSYEPTGPFGAKSIGEIVINTLSPAIANAVYNAVGVRIRELPI
                     TDEKIFIGIMGGR"
     gene            2783..3745
                     /locus_tag="ACZPCL_05010"
     CDS             2783..3745
                     /locus_tag="ACZPCL_05010"
                     /inference="COORDINATES: protein motif:HMM:NF016629.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HIR1161870.1"
                     /translation="MGTKDIETAIFFDDDRRYADLLNGYIFDGKQVIMEKQLYSQDTR
                     ETGKSQKDSWGVQKRKKIQRFRDGVKRVAFGTSFTIIALEHQDKIHRGMPVRVMVEDA
                     LSYDRQMKKLQKQNREKGVLNKKDYISSLTDTDVLTPVVTIVIYYGEEPWNNSKDIYD
                     ILAHKDLPEEIKTFINHYPIHVLDVRRFTNEECFQTDVREVFGFIRRSLDKEAVKQYL
                     KEREEQFTNIDEDAYDLIAAMTDTKQLQMVKDKYRSKEGGINMCKGLLDWVEESKIEG
                     KSAGELTILKLITAMQLDGRTSDITRLSSEPEFLEGMKKHYHIQ"
     gene            3858..4310
                     /locus_tag="ACZPCL_05015"
     CDS             3858..4310
                     /locus_tag="ACZPCL_05015"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1161871.1"
                     /translation="MIEATSCGGVVIFRGKILVLYKNYKNKYEGWVLPKGTVEAGEEY
                     KETALREVKEETGVSASIIKYIGKSQYTFNTPQDVVEKDVHWYLMMADSYYSKPQREE
                     YFLDSGYYKFYEAYHLLKFSNEKQILEKAYNEYLDLKRSNLWGSKKYF"
     gene            complement(4389..5531)
                     /locus_tag="ACZPCL_05020"
     CDS             complement(4389..5531)
                     /locus_tag="ACZPCL_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1161872.1"
                     /translation="MICIKNGTIHTAVDPQPFTGDILIGGGKILKIASHIDDDYEQLI
                     EAAGLNIYPGFVEAHCHTGLDGYGNGVPGQDTNEWNDPVVPQLRAIDGINPMDPCFEM
                     AAKAGITCFATGPGSSDAIGGTFAAVKPMGKRIDNMILKYPIAMKCAFGENPKRRYMT
                     SRMSTRMTNAALIREALQKAALYMVKKEAAGDDVSKLPAFDQKSESLIPVLKGELPLK
                     AHAHQANDIFTAIRIAKEFHAKLTLEHVTEGHLIVDELVKENLPLAVGPTFSHATKVE
                     LLNKSWTTPGILAKAGCHVSIISDAPVTPLHDLPLYAGMAMKAGMDPYDALRAITINA
                     AEHIGVADRVGSLETGKDADLVICKGSPMEISGVVKYVFINGELVE"
     gene            5843..6325
                     /locus_tag="ACZPCL_05025"
     CDS             5843..6325
                     /locus_tag="ACZPCL_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LeuD/DmdB family oxidoreductase small subunit"
                     /protein_id="HIR1161873.1"
                     /translation="MAKGTIFKFHNDLDTDQIIASQYLLLPNLEEMKEHAFESLDPDF
                     PKKVKPGDFVVGGENFGCGSSREQAPGVLKALGVQAIVAKSFARIFYRNAINIGLPVI
                     VCKELPDEVKTGDTMELSMSDGTAQANGKTYSCTKLPEYMQNILNQGGLIASLNREEE
                     "
     gene            6330..7583
                     /locus_tag="ACZPCL_05030"
     CDS             6330..7583
                     /locus_tag="ACZPCL_05030"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR1161874.1"
                     /translation="MGMTIAEKIIAAAAGVESVKPGDIHTVTLDRLMSNDGTTHLTVD
                     MYRNKLKNPHIADAKKVVFIVDHNVPSDCPKTAASQKKMRDFAKENNIDFWEGKGVCH
                     QVMMENYVCSGELIFGADSHTCSYGALGAFGTGVGCTDFLYGMVTGKSWVLVPETVKF
                     NLVGKLPEGVYARDLILTIIGEIGANGCNYQVMEFTGEGAKSLSISERMVLCNMAVEA
                     GAKTGIFEADEKALEYLEAHGRKPKAVYKSDPDAVYAREYTFDLSKVRPVAAKPDFVD
                     NVVPAEEVRGIKIDEAFLGSCNNGRIEDLRVGASIIKGKKVAEKVRFLVVPASQTVYR
                     QALKEGLIDIFMEAGAIVMNPNCSVCWGSCQGVIGENEVLISTGTRNFKGRAGHPSSK
                     VYLGSAATVTASAIAGEIALAEDVM"
     gene            7636..8127
                     /locus_tag="ACZPCL_05035"
     CDS             7636..8127
                     /locus_tag="ACZPCL_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LeuD/DmdB family oxidoreductase small subunit"
                     /protein_id="HIR1161875.1"
                     /translation="MDKMIGKVWVLGDDIDTDIIIPTEYLALKTIDDMKQYGFSPLRP
                     ELAAQIKPGDIIVAGKNFGCGSSREQAPEIIKALGVQCVIAKSFARIFFRNSINNGLL
                     LIEQPTLYDDIKEGDTISVVMNEHVDYNGKTYPIASLPENLMSIIRAGGLVKAMRKLN
                     GLD"
     gene            8153..9397
                     /locus_tag="ACZPCL_05040"
     CDS             8153..9397
                     /locus_tag="ACZPCL_05040"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR1161876.1"
                     /translation="MGKTVIEKIIEHNTGKEVKPGDIVTVNVDRVMIHDIFIPFVAEK
                     FKEMGFEKLWDPDKVVLIYDHLVPASQLDDTRHFHTGDAFAEKYGMTHVHRSDGICHQ
                     LMTEAGYVKPGNIAFGTDSHTTTYGCVGAFSSGIGYTEMASILGTGTMWIKVPETIKV
                     VINGKLPENVMSKDVILRLIGDLGADGATYKALEFSGSAVENMTVASRMTISNMAIEA
                     GAKCALFTPDEKTAEYCDITLNDYQKSLVGDQDATYCKVMEYKAEDFVPVMACPSQVD
                     KIRNVSELEGTEIDQVFIGSCTNGRLEDLAAAAEVLKGKKVANFVKLIVTPASRKIYR
                     QADEMGILDTLAEAGAIITHPGCGLCCGRTGGILTDGERVVATNNRNFLGRMGTSKVQ
                     IYLASPKTAANCAIAGKIVSPQ"
     gene            complement(9502..10206)
                     /locus_tag="ACZPCL_05045"
     CDS             complement(9502..10206)
                     /locus_tag="ACZPCL_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1161877.1"
                     /translation="MSEISKEIGQNIRRTRKMRKITHQQLAQAIGKSQSAISKYESGE
                     IAVDIDTLYAIANALQVHIETLLYFPNTATSSSTLKECPAFFRNVKNLYGYVYDGRIN
                     RIGRRLFELHPEENGLTKVMMYMNFEDYDHYQNCENTYKGYMEHFDAVTNITLQNRDV
                     EMETAYIQILAPTLNAETKWALFTGLSTRPIMPIARKLLLSKNRLCENKELENQLKVS
                     KEDIKQLKLYHMYTVT"
     gene            10482..11675
                     /locus_tag="ACZPCL_05050"
     CDS             10482..11675
                     /locus_tag="ACZPCL_05050"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR1161878.1"
                     /translation="MNYTKEYFDFDLDRKNTRSMKWDDCNSKFGVDESTAMIPMWIAD
                     MDFRSPKEVIDAVIDRASLGCYGYTTKPDCFYETIINWVDRRYHWKIKKEWIIFTPGV
                     IPGYTITIQNFTKPGDGIIVQTPVYYPFMDGVVNNGRKLVYNPLIEKEGDWFMDFEDL
                     ERKVKDPDNKLLILSNPHNPVGRAWTAEELTSLGNLCAENDVLLISDEIHADLMMKGI
                     THTSVGTLSEKIKKNTIVHYAPSKTFNLAGLQTAYAVIPDDELRETYVKGLNANRIFN
                     MNWFGSAALEAAYDKSGNYVDTLCDYVNDNMDHMVQYVQERLPMLHMKKSEATYMVWV
                     DFRGTGMSTEEIESFIDQKAHIGVDKGSWFGPGGEGYLRFNLACPRKILDSALYQLET
                     ALKQI"
     gene            11790..12389
                     /locus_tag="ACZPCL_05055"
     CDS             11790..12389
                     /locus_tag="ACZPCL_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161879.1"
                     /translation="MELAGVLIAVLLLIFLIYKRLSLIPATLICVAVLALINGFSYMD
                     LFINHYGVSLAGFVGKYFLVFVTNALFGKVMEETLLASVFSKMIGKLFGDKNAVFGAM
                     LATAILSYGGVSVFVIVFTVYPIFLATFRKADLPGKYIPACIMSSSCTFALSLLPGGA
                     QLNNIIPVQYLGTTPAAAAGIGLLCCGYGIYIRIFFLGI"
     gene            12352..13065
                     /locus_tag="ACZPCL_05060"
     CDS             12352..13065
                     /locus_tag="ACZPCL_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161880.1"
                     /translation="MAFIFVYFSWEFKKARQRGEHFEINPSIKERITKFEMDAGIPGP
                     LSVLPLVMIILLINIAQLDLSYAVLAGTGVALFIGWKNIPDKLRIINESAKLVGPAVI
                     TTAVSVGFGGAVLACNGAETVLEAISALPVPPNLSFSIAASFAGAMTGNGGGGMDVAM
                     NLLSGQYLAAGLEPELIHRLGAVATAGFSCLPHNGMQITIMDTCGYTAGQCYKYIFIS
                     TVLCSAGCLLLANIISCLL"
     gene            13095..14267
                     /locus_tag="ACZPCL_05065"
     CDS             13095..14267
                     /locus_tag="ACZPCL_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR1161881.1"
                     /translation="MKITKIDLLYCDPVEGGWRQSLCRIYTDEGIYGDGEVALSYGGA
                     SQAAFAMLKDMANRLIGMNPLDHEVIWQKLYQGCFWSYNGGPIVFGGISAFDIALWDI
                     KGKVYGQPLYRLLGGKHRDTLKAYASQLQNGWGIGRKTARTPADYARQEEIAVEKGFK
                     AVKYNFLTFREDEGRYPRTEQTAYLDPEYLDLAEARIAAVREAVGSKVDIILENHCYT
                     DAQSAVQFGNMAKEYGILYYEEPVVPHEDTLAYVHQQTGIPVASGERIYSRWQFKKML
                     DKQAIQVIQPDLGNSGGVTEVKKICDMSYVYEAGVQIHVCGTPIVTAASLHMEAALPN
                     FVIHEYNVNTEMPQMTSLALHHYEPVNGLFTVPELPGIGNEIAPEAFKRSQVVTIE"
     gene            14462..15637
                     /locus_tag="ACZPCL_05070"
     CDS             14462..15637
                     /locus_tag="ACZPCL_05070"
                     /inference="COORDINATES: protein motif:HMM:NF016083.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HIR1161882.1"
                     /translation="MVFAVIIVLLAIGRPLYQAIAGGMIVTAGLFHIPVTVIVTKIAS
                     VIINWSSLSVLLSLYLITFLQRILESRSQIRLAQQDLNGIFHNRRINTAGAAFFIGLL
                     PSAASMILCADIVKDATEGYLDPKEQAFTASWFRHIPESVLPTYTAVLLMSNLSGVEI
                     SEFIVYMIVPVIALAGLGYAVYLHRIPNDTGTPASTNRLADFAHLIQHLWSLLLILIL
                     ILVFHFQVVTSVLVSIALCIIIYRVTLDEIRKFIFAAFEKKMLGNTFLVLCLKELIAH
                     TGVLLLLPDAMAKLPLPTYLIFGLLFFTATVISGSTGAIAMGTPLAFAAIPGGAPLMA
                     YLMCMAHAASQISPTHVCLVVAADYFHVTLGDIVKKTLPVSIAFCVLMTLYYNVIKL"
     gene            15676..16050
                     /locus_tag="ACZPCL_05075"
     CDS             15676..16050
                     /locus_tag="ACZPCL_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013282038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR1161883.1"
                     /translation="MYKRKIEKDIRCPLEYGLEVFGGKWNSRIICVLAAKEVLRYSEL
                     RREMGNITDAVLAAALKELITNGIVKRQSYDEIPPRVEYSLTEKGNSVIPILQSICRW
                     AGVFYKEDSENEMIQCKRCDYR"
     gene            16173..17030
                     /locus_tag="ACZPCL_05080"
     CDS             16173..17030
                     /locus_tag="ACZPCL_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024874.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1161884.1"
                     /translation="MNITGKGMKFRWLNNAGFEMELSSGKHLLVDPWLDEAQIYPISI
                     DEIERADYVLLTHTHDDHSDSIGRIQKRFPQAAIFVGDLSAEALCKEYDLNVERLFRI
                     RGGEEYEFDDVKIEVISARHTESKGGNYWDRGYCIQKDGSRRETMWYGSLEMYNFRIT
                     DVNGYRAVVWGGMTTEEQIHRMEKYSGNEIAFMHVSPKQDHQMFARLVQAINPKAVIP
                     HHYDIWETLFAAKPELLADMKLPADKVNAEGVLDVIRQNIKENCPDVAFFIPEHHKWY
                     QFGYGIMEA"
     gene            17116..>17375
                     /locus_tag="ACZPCL_05085"
     CDS             17116..>17375
                     /locus_tag="ACZPCL_05085"
                     /inference="COORDINATES: protein motif:HMM:NF015509.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR1161885.1"
                     /translation="MSTFWILLEQMELFVIYILAGVILVKSRLFNRETLQPISKFVLR
                     MGLPLLIFTNIINGVERNVLLSSGSVLMLAFLFYVAMFFISMG"
BASE COUNT         5230 a         3245 c         4208 g         4692 t
ORIGIN      
        1 gccggatgta ttgccaacgc tttgcttgac caggatgtgc cggtgtatac tgatgacctg
       61 gcattaaagg actgcctgat cgtaaaagtg cttagaagcc cacatgccca tgcactgatc
      121 gaagagatcg actgcaaggt agcttccaga gtaccaggga tcgaatgtat ccttacatgg
      181 aaggatgttc cgcagaagcg ttttaccatg gccggacaga cgtatcctga gccaagtcct
      241 tatgaccgtc tgatcctgga ccagagagtc cgttttgtag gtgatgcagt ggcgatcatt
      301 gcaggtgaga cagaagctgc agtagaccat gcaatgcgtc ttattaaagt gaaatatcag
      361 gtgttagagc ctgttttaga tatgcatgac tccaaggacg gcaagatcct tgtacatccg
      421 gaggacagct ggaaagccct gtgtaatgta ggcgctgata ataagagaaa cttatgtgcc
      481 agtggcggag agactcatgg ggatctggag gcggcatttg caggctgcag ccacattgtg
      541 gaaaaaacat accacaccaa ggcgaaccag caggctatga tggagacttt ccgtgccttt
      601 acttatatgg atgtatacgg acgtttaaat gtagtagact ccacccaggt tacattccat
      661 gtgcgccgta ttctggctca tgcactggat gtgccaaagt caaagatccg cgtgatcaag
      721 ccaagaatcg gtggcggttt tggcgcaaag cagactgttg tagcagaggt ttatccggct
      781 atcgttacca tgaagacggg aaaaccggct aagatcattt acagccgtta tgagtcccag
      841 atcgcctctt ctccacgtca cgaaatggaa gtaaaggtaa agatcggctg cgatgacaac
      901 ggtattttac aggcaatgga tgtgtataca ttgtccaata caggtgcatt tggtgaacat
      961 ggaccgacca ctgtaggtct ttccggccat aagtccattc ctatgtacgg cacaccaaaa
     1021 gcattccgct ttgcttatga tgtcgtttat accaaccgca tgtctgcagg cgcttaccgt
     1081 ggatatggcg caacgcaggg tatttttgct gtagagtctg ctatggatga gctggcagcc
     1141 cagatgggaa tgaacccgat cgagcttaga aagaaaaata tggtccgcca gggacaggtg
     1201 atgcctgctt actacggcga gactgccaat agctgtgccc ttgaccgctg cgtggacaag
     1261 gttatggaga tgatcggctg ggatgaaaaa tatccgagaa aagatatggg aaatggaaag
     1321 gtaagagcag ttggtcttgc aatggctatg cagggctccg caatttccgg tgtagacgtg
     1381 ggaagcgtta ccattaaggt aaatgatgac ggcttttaca gcatgaccat tggtgcggct
     1441 gatatgggta ccggctgtga caccacactg gcccaggtag cagcagagtg tctggactgt
     1501 gagctggatg atatcgtagt ttacggtgtt gatacggata tttccccata tgactccggt
     1561 tcctatgctt ccagtactgc atatttaaca ggtatggcag tagtaaagac ctgtgagtca
     1621 ctgaaaaaga agatattaaa gaaggcggca gaatatctga attgctctga ggatgaactg
     1681 gagtttgcag gaaagacagt gcgcagatta aattcccctg aaggcgaacc ggcagagatc
     1741 acattaaagg acattggaaa cagagccatg tgctttaatg aagaagccct ccaggctaca
     1801 gaatcccatt cttcacctgt ttctccgcca ccatttatgg caggcgctgc agaagtggag
     1861 atcgacaagg aaaccggtca tattgagctg cttcagtttg cagcagctgt agactgtgga
     1921 accccattaa acgagaacct ggcaagagta cagacagaag gtggcctggc acagggaatc
     1981 ggtatggcaa tgtacgagga tgtaacctat tccgaccatg gccgtgtcca tgaaaattcc
     2041 ttcatgcagt acaaagtacc aagccgtctg gatattggaa aagtccaggt agaatttgaa
     2101 tgcagctacg agccaaccgg cccatttggc gcaaaatcca tcggtgagat cgttatcaac
     2161 accctatctc cggctattgc aaatgcagtg tataatgcag taggcgtaag gatcagggaa
     2221 ctgcctatta cagatgagaa gatctttatt gggatcatgg gtggccgatg agcgcaggga
     2281 agaacaagcg acgccgaagg cggcatttgt ttttccaaag ccattaacga aggtccagcc
     2341 tgcgtaagca ggcagtagag cacgttagtg cggggacctg agtggacggc tcgtgagagc
     2401 aataataaaa ccagccttgc agataacgca tgagtgcaca aagatgcata aatgcggtct
     2461 gtgaggctat ttttatgcag ggggaaaatt gtggtggatt tttggatccc gctatgatat
     2521 actatggtta catttcttaa tcataaagaa taatatttca aaactatgta atgtttaatg
     2581 cgttgcatat ctgtattcag gcagcttcag ctaaaaatca tggatcaaaa gcaaaaattt
     2641 aaaagaagtg gaaatggtct caggaaattc tgtttaaaaa caggcacggg ggtgggggat
     2701 attgaaattt ataactggat tttgtataat aaatattaga aactgcccct ttgccttgca
     2761 acagcaagaa aggggagaat aaatggggac aaaggacata gaaacagcga tatttttcga
     2821 tgacgacagg cggtatgcgg atctgttaaa tggatatatc tttgatggaa agcaggttat
     2881 aatggaaaaa cagctttata gccaggatac aagagaaaca ggaaaatcac aaaaagacag
     2941 ttggggtgta caaaaaagga aaaagataca gcgtttccgt gatggagtaa agagagttgc
     3001 gtttggtaca tcatttacga tcattgccct ggaacaccag gataagatcc acaggggaat
     3061 gccggtgcga gtgatggtag aagatgcttt gtcttatgac cggcagatga aaaagctgca
     3121 gaaacaaaac cgggagaaag gtgttttaaa caaaaaagat tatatcagca gtctgacaga
     3181 cacagacgtt ttaacaccag ttgtaaccat tgtgatctat tatggtgaag agccgtggaa
     3241 caacagcaaa gatatttatg atattttagc ccacaaagat cttccggaag agataaaaac
     3301 atttatcaac cactatccga tccatgtttt agatgtccgc agatttacaa acgaggaatg
     3361 ttttcaaaca gatgtgagag aggtatttgg atttatccgc cgttcattag acaaagaagc
     3421 agtaaaacaa tatctgaaag aaagagaaga gcagtttaca aacatagatg aagatgccta
     3481 cgacctgatc gcagccatga cagacaccaa acagctgcag atggtcaaag ataagtatcg
     3541 tagtaaagaa ggaggaatca atatgtgcaa aggacttctt gattgggtgg aagagagcaa
     3601 aatagaggga aaatctgccg gtgaactgac catcttaaaa ttaatcaccg ccatgcaact
     3661 agatggccgc accagcgata tcacccgtct gtcatcagaa ccagagtttt tagaaggaat
     3721 gaaaaagcat tatcacatcc aataataaaa ccattataag catctagcga ctcagatttc
     3781 cttcttgcat ataaccctca aacgttgtat actaaaacca gttattcaga ttcacaccaa
     3841 aaccgggagg aattgcaatg attgaggcaa cgagctgcgg cggtgtggtt atatttcgag
     3901 ggaagatcct ggtcctgtat aagaattaca agaacaaata tgagggctgg gtcctgccta
     3961 agggaacagt agaagctggc gaagaataca aagaaacggc tcttagggaa gtaaaagaag
     4021 agaccggagt aagcgcttct atcatcaagt acattggaaa gagccagtac acctttaata
     4081 cgccccagga tgtggtagaa aaagatgtac actggtatct gatgatggct gacagctact
     4141 acagtaaacc acagcgggaa gaatactttc tggattccgg gtattacaaa ttttacgaag
     4201 catatcatct gctgaagttt tccaatgaaa agcagatcct tgaaaaggct tataatgaat
     4261 atctggacct taaaagaagt aatctttggg gaagcaagaa gtatttttaa aaacagaata
     4321 agaagaaaat aaaaactgct gtatacggga gtgataaaac tgtatacagc agtttttggt
     4381 atataatgtt attccaccaa ttccccatta ataaacacat acttcaccac tcctgaaatt
     4441 tccataggag agcctttgca gatcacaagg tcggcgtctt ttccggtttc cagggaacct
     4501 acccggtcag caacaccgat gtgttcggcg gcattgatgg tgatggcccg cagggcatcg
     4561 tagggatcca taccggcttt catggccatg cctgcgtaaa ggggcagatc atgcagcggg
     4621 gtaacaggtg catcggaaat aatggaaaca tggcagccag cttttgccag gatgcctgga
     4681 gtggtccagc ttttatttag cagttctact ttggtggcat ggctgaaagt aggtcctaca
     4741 gccagaggaa ggttttcttt caccagttca tctacgatca ggtggccttc tgttacatgt
     4801 tccagagtca gtttggcgtg aaattccttt gcaatgcgga tggcagtgaa aatatcattt
     4861 gcctgatgtg catgggcttt taaaggaagt tcccctttta aaacaggaat cagggattct
     4921 gatttttgat caaaagcagg cagcttggag acgtcatcac cggcggcctc tttttttacc
     4981 atgtataatg cagctttctg cagtgcttcc cggatcaaag ctgcattggt catacgggtg
     5041 gacatgcggc tggtcatata gcgtcgtttt ggattttcac caaaagcaca tttcatggca
     5101 atagggtatt taaggatcat attatcaata cgttttccca tgggtttcac agctgcgaaa
     5161 gtgcctccaa tggcatcaga acttcctgga cctgtggcaa agcaggtgat cccggctttg
     5221 gcagccattt caaagcaggg atccatagga ttgatgccgt caatagcccg cagttgtggc
     5281 actactgggt cattccattc gttggtatct tgtcctggta cgccgttgcc gtagccatca
     5341 agacctgtat ggcagtgtgc ctcaacaaaa ccaggataaa tattaagtcc ggcagcttcg
     5401 atcaactgtt cataatcatc atcaatgtga ctggcgatct ttaagatctt tccgccgcct
     5461 ataaggatat ctcctgtgaa tggctgcgga tcaactgctg tgtggatcgt tccgtttttt
     5521 atgcatatca tgtaagtcta cctccttaag gattcgatat aaagtcatta taactcggat
     5581 gtacatgaaa ttacagtaaa agaagtgtga gagagaaaag cacgcagcaa ttcggtatca
     5641 gcggggaaaa atacttttga tctcaatctc ttttctatga tacaggttat ctgtggggcg
     5701 cattctataa cttacagtta ttaaatgtat caataaccac catttccatt acggcccatc
     5761 ctatggtata ctacatatca gtacaaaaca cagaaaaaac aaaacccagg cagggcggct
     5821 ttaagaaagg aatacatata tcatggcaaa aggaaccatt tttaaatttc ataatgacct
     5881 tgatacagat cagatcatcg catcacagta tctgctttta ccaaacttag aagaaatgaa
     5941 ggagcatgca tttgagtctt tagatccaga ttttcctaaa aaagttaaac cgggagattt
     6001 tgttgtaggt ggcgaaaact ttggatgcgg ttcttccagg gaacaggcac cgggcgtttt
     6061 aaaagcatta ggtgtacagg ctatcgtcgc aaaatccttt gcaaggattt tctacagaaa
     6121 tgcaatcaat atcggacttc ctgttattgt ctgtaaggag ctgccggatg aggtaaagac
     6181 cggcgatacc atggaacttt ccatgtcaga tggaactgcc caggcaaatg gcaagaccta
     6241 ttcctgcacc aagctgccgg aatacatgca gaatatttta aaccagggcg gactgatcgc
     6301 ctcattaaac cgtgaggagg aataggaaaa tgggaatgac gattgcagaa aagatcatag
     6361 cggcagcagc cggcgtagaa agcgtaaaac cgggagatat ccatacagta acattagatc
     6421 gtttaatgag taatgacggt actacacatc tgactgtaga tatgtaccgc aataaattaa
     6481 aaaatccaca tattgctgat gcaaaaaaag tggtattcat cgtagaccac aacgtacctt
     6541 ctgactgccc gaaaacagca gcatcccaga agaagatgcg tgatttcgca aaggaaaata
     6601 acattgattt ctgggaaggc aaaggtgtat gccaccaggt gatgatggaa aattatgtat
     6661 gttccggtga gctgatcttc ggtgcagaca gtcatacctg ttcttatggc gctttaggtg
     6721 cttttggtac aggcgttgga tgtacggatt ttctttatgg aatggtaact ggaaaatcct
     6781 gggtattggt tcctgaaact gtaaagttta acctggtagg aaaactgccg gaaggtgttt
     6841 atgcaagaga cctgatctta acaatcatcg gtgagatcgg tgcaaacgga tgtaattatc
     6901 aggtaatgga gtttaccgga gaaggcgcaa aatccttaag catcagtgag cgtatggtac
     6961 tttgcaatat ggcagttgag gccggtgcaa agacaggtat ttttgaggct gatgaaaagg
     7021 cattagaata tctggaagca catggcagaa agccaaaggc tgtatacaag agtgatccag
     7081 atgcagtata tgcaagagaa tatacctttg acttatccaa ggtacgtcct gtagctgcaa
     7141 aaccggattt tgtggataat gtagttcctg cagaagaagt tcgtggaatt aagatcgacg
     7201 aagcattctt aggttcctgc aacaatggac gtattgaaga tttaagagtg ggtgcttcta
     7261 ttatcaaggg caagaaagta gcagaaaagg tacgttttct ggtagttccg gccagccaga
     7321 ctgtttaccg ccaggcatta aaagaaggcc tgatcgatat cttcatggaa gcaggcgcta
     7381 ttgtcatgaa cccgaactgc agcgtatgct ggggaagctg tcagggcgtg atcggtgaaa
     7441 atgaagtcct gatcagtaca ggcaccagaa acttcaaggg ccgtgcagga catccaagct
     7501 ctaaggtata tttaggttct gcagctacgg ttaccgcttc tgccattgca ggagagatcg
     7561 ctctggctga ggatgtgatg taatgcgtgg ttcgtgagtt tacgaaataa atcaaagata
     7621 tgaggatata gaaaaatgga taaaatgata ggaaaggtat gggttttagg agatgatatt
     7681 gatacggata tcattatccc aacagagtac cttgcattaa aaaccataga tgatatgaaa
     7741 cagtacggat tttccccgct gcgcccggaa ctggcagcgc agatcaagcc gggagatatt
     7801 attgttgcag gaaagaactt tggctgtggc tcctccagag agcaggcgcc ggagatcatt
     7861 aaggcactgg gagttcagtg cgtgatcgca aaatcctttg caaggatctt cttccgcaac
     7921 tccatcaaca atggtctgct tctgatcgag cagccaactc tctatgatga catcaaagag
     7981 ggagatacca tctctgttgt aatgaacgaa catgtggatt acaatggaaa aacataccca
     8041 atcgcctctc ttccagagaa cctgatgagc attatccgcg caggcggtct ggtaaaggct
     8101 atgcgcaaac tgaatggtct ggattaagaa agagaagtga ggtgtgtaag aaatgggaaa
     8161 gacagtaatt gaaaagatca tagagcataa taccggcaaa gaagtaaagc cgggagatat
     8221 tgtgactgta aatgtggacc gtgtcatgat ccatgatata tttatccctt ttgtagcgga
     8281 aaaattcaag gaaatgggct ttgagaagct ttgggacccg gataaagtgg tcctgatcta
     8341 cgaccacctg gtacctgcaa gccagttaga tgataccaga catttccata caggtgatgc
     8401 ctttgctgaa aaatacggca tgacccatgt acacagaagc gatggtatct gccaccagct
     8461 gatgacagaa gcaggttatg taaagccagg taatattgca tttggtacag acagccatac
     8521 aactacctac ggctgtgtag gcgcattctc ttccggtatc ggctatacag aaatggcaag
     8581 tatcttagga accggaacca tgtggatcaa ggtgccggag accattaaag ttgtgatcaa
     8641 tggcaagctg cctgaaaatg ttatgtcaaa ggatgtgatc ttacgtctca tcggtgatct
     8701 gggagcagac ggtgctactt ataaagcact ggaattttcc ggaagtgcag tagagaatat
     8761 gactgttgcc agccgtatga ccatttccaa tatggctatt gaagcaggtg ccaagtgtgc
     8821 tctgtttaca ccagatgaaa aaacagcaga atattgcgat attaccttaa atgactatca
     8881 gaagagcctt gtaggagacc aagatgcgac ctattgtaag gtaatggaat acaaggcaga
     8941 agattttgta ccggttatgg catgtccgtc ccaggtagac aagatccgca atgtaagcga
     9001 actggaagga acagagatcg accaggtatt tatcggttcc tgcaccaacg gccgtttaga
     9061 ggacctggca gcagctgcag aagttttaaa aggcaagaag gtagcaaatt tcgtaaaact
     9121 gatcgtaaca cctgccagcc gcaagatcta cagacaggca gatgaaatgg gtatcctgga
     9181 tacactggca gaggctggtg ctatcatcac ccatccaggc tgcggactgt gctgcggaag
     9241 aacgggcggt attttaacag atggtgaaag agtagttgca accaataacc gcaacttctt
     9301 gggccgtatg ggaacttcaa aggtacagat ttatctggct tctccaaaga cagcagcaaa
     9361 ctgtgcgatt gcaggtaaga tcgtaagccc acagtaatag gaattaaaca taataccaat
     9421 ataaataaaa agacatattt tcattcccga aagttatttt gggaaaagaa aatatgtctt
     9481 tttattttac aaacacagtg atcaggtcac cgtatacata tggtacaact taagctgttt
     9541 aatatcttct ttggatactt tcagctgatt ttccaattct ttgttttcac ataatctgtt
     9601 ttttgagagt agaagttttc gtgcaatagg catgatcggt cttgtagaca ggcctgtaaa
     9661 taaagcccat ttggtttctg cgtttaatgt aggagccagt atttgtatat aggcagtttc
     9721 catttccaca tctcgatttt gcagagtgat atttgtaact gcatcaaaat gttccatgta
     9781 gcctttataa gtattttcac agttctggta atgatcataa tcttcaaagt tcatatacat
     9841 catgaccttc gtaaggccat tttcttccgg atggagttca aaaaggcgtc gcccgatccg
     9901 gttgatcctt ccatcgtaaa cataaccata caagttttta acatttctga aaaaagcggg
     9961 gcattctttt aaagtggaag aggatgtggc agtattggga aaatacagaa gggtttcaat
    10021 atgcacctgc agtgcgttgg caatggcata cagagtgtct atatccacgg ctatttcacc
    10081 tgattcatat tttgatatag cggactggct tttgccaata gcctgagcca gctgttggtg
    10141 ggttattttc cgcattttac gtgtacggcg gatattctgt ccgatttctt tgcttatttc
    10201 tgacatatgg tttccttttt tattccctaa aataatattt tttaacgatt taggatcgcg
    10261 gacacaaatg atgtgcagtt tttcagcttt tattgtccga aattaatcgt gtcacggaaa
    10321 gaaaatgttg aagttattat ccacaggaat attttgcttt tttgacgggg acttttccta
    10381 ttgctataat tttaaacaag acggggaaat aagtcaataa aagtctataa ctgcaggcgc
    10441 cgcagatata tataataagg tattcaagga gagatgtaat tatgaattac acaaaggaat
    10501 attttgattt tgatctggat cgtaaaaata cccggagtat gaaatgggat gactgcaatt
    10561 caaaattcgg cgtagatgaa tctacagcga tgatcccaat gtggatcgca gatatggatt
    10621 ttaggtctcc gaaagaagtg atcgacgcag taatagaccg cgccagtctt ggatgttatg
    10681 gttatacaac aaaaccagac tgtttttatg aaacgattat taactgggta gatcgccgtt
    10741 atcactggaa gataaaaaaa gaatggatca tttttactcc aggtgtgatt ccgggataca
    10801 ccattacgat ccagaatttt acaaagcccg gggacggcat tatcgtacag acaccggttt
    10861 attatccatt tatggatggt gttgttaata atgggcgtaa gctggtttat aatcctctga
    10921 tagaaaaaga gggagactgg ttcatggatt ttgaagatct ggaaagaaaa gtaaaagatc
    10981 cggataataa acttctgatc ttatctaatc ctcataatcc tgtaggacgt gcatggacag
    11041 cagaagaatt aacgagcttg ggaaatctct gtgcagaaaa tgatgttttg ttgatttctg
    11101 atgaaattca tgctgatctt atgatgaaag gaatcacgca tacatctgtt ggtacccttt
    11161 cagaaaaaat aaaaaagaat acaattgtcc attatgcccc aagcaaaacc tttaacctgg
    11221 ctggccttca gactgcttat gcagttatac cggacgatga actgcgtgaa acgtatgtaa
    11281 aaggattaaa tgcaaaccgt atttttaata tgaactggtt tggttctgct gctttggaag
    11341 cagcttatga taaaagcggt aattatgtgg atacattgtg tgactatgta aatgacaata
    11401 tggatcatat ggttcagtac gttcaggaaa gactgcctat gcttcacatg aaaaaatctg
    11461 aggcaactta tatggtatgg gtagattttc gcggaactgg gatgagtaca gaagaaattg
    11521 aatcatttat tgaccagaaa gcacatattg gagtagataa aggatcctgg tttgggccgg
    11581 gcggagaagg ttatctgcgg tttaatctgg catgcccaag gaagatcctt gatagtgctc
    11641 tttatcagct ggaaactgca ttaaaacaga tttgactggt gcttataaat ttcttagtaa
    11701 cttataaaat atatagaaaa ttgtagaaaa taaacgtctt ttctataatg aaacaaagaa
    11761 aaaagcatgg ttagtaggag gtgatacagg tggaactggc aggtgtattg atcgcggtac
    11821 tgctgctgat ttttttgatc tataaaagac tgagcttgat cccggcaaca ctgatctgtg
    11881 tggcggtact ggcattaatc aatggctttt cttatatgga tctgtttata aatcattacg
    11941 gcgtttcact tgccggattt gtgggaaaat attttctggt gtttgttaca aatgctttgt
    12001 ttggaaaagt aatggaagaa acgctgttag catctgtatt ttcaaaaatg atcggaaaac
    12061 tttttggaga taaaaatgct gtttttgggg caatgttagc aactgcgatc ctttcttatg
    12121 gaggcgtcag tgtatttgtt attgtattta ccgtatatcc catttttctt gcaacatttc
    12181 gcaaggcaga tcttccggga aaatatattc cagcctgcat tatgagcagc tcctgtacat
    12241 ttgccctttc gctgcttcct ggaggagccc agttaaacaa tattattcct gtacagtatt
    12301 tgggcacaac accggctgca gctgccggga tcggtctgct ctgctgcggc tatggcattt
    12361 atattcgtat atttttcttg ggaatttaaa aaagcaaggc aaagagggga gcattttgaa
    12421 ataaatccgt ctattaaaga acggatcaca aagtttgaaa tggatgccgg gattccggga
    12481 ccattatcag tcctgcctct tgtaatgata attttactga tcaatattgc gcaattagat
    12541 ctttcttatg cagtgctggc aggaactggt gtggctcttt ttattggctg gaaaaatatt
    12601 ccggataaac tgcgtattat caatgaaagc gcaaaattag taggacctgc tgttattaca
    12661 acagctgtat ctgttggatt tggcggtgca gttcttgcat gtaatggagc ggagactgtg
    12721 ttagaagcaa tatcagcttt gcctgtaccg cctaatcttt cctttagtat tgctgcatct
    12781 ttcgcaggag caatgactgg aaatggcgga ggcggaatgg atgtggcaat gaacctgctt
    12841 tccggacaat atctggcagc tgggcttgag ccggagctga tccaccgcct gggagcggta
    12901 gcaactgccg gattcagctg tctgccccac aacggaatgc agatcacgat catggataca
    12961 tgcggatata cagccggaca atgttataaa tatattttta tttcaacggt actttgttcc
    13021 gccgggtgtc tgctgttagc aaatataata agctgtttac tgtaataaaa atacatagat
    13081 gaaggagact gggaatgaaa attacaaaaa tcgatctttt atattgcgat ccggtagaag
    13141 ggggctggag acaatcttta tgccgtatat acacagacga gggaatttat ggagatggag
    13201 aagtggcatt atcttatgga ggcgcttcac aggctgcatt tgccatgtta aaagacatgg
    13261 caaaccggct gattggaatg aatcctttgg atcatgaagt aatctggcag aaactgtatc
    13321 aggggtgttt ctggagttac aatggaggtc ctattgtatt tggcggtatc agtgcctttg
    13381 atattgcctt gtgggatatt aaaggaaagg tgtatggaca gccgctgtac cgtcttttag
    13441 gtggaaaaca cagagatacc ctgaaagctt atgcaagcca gctgcaaaat ggctggggta
    13501 ttggaagaaa aacagcaaga acaccagcag attacgcgcg tcaggaagaa attgcagtag
    13561 aaaagggatt taaagcagta aaatacaatt tccttacttt tagagaagat gaaggaagat
    13621 atccaagaac agagcagaca gcttatctgg atccagaata tttggatttg gcagaagcga
    13681 ggatcgcagc tgtaagagaa gcggtaggtt ctaaggtaga tattattttg gaaaaccatt
    13741 gttatacaga tgcccagtca gcagtccagt ttggaaatat ggcaaaggaa tatggcattc
    13801 tctattatga ggagccggta gtccctcacg aggatacact tgcttatgtg catcagcaga
    13861 cgggaattcc agtagccagc ggtgagcgga tttacagcag atggcagttt aagaaaatgt
    13921 tggataaaca agccattcag gtgatccagc cggatcttgg aaacagcggt ggtgttacag
    13981 aggtgaaaaa aatctgtgat atgtcctatg tatatgaagc cggtgtccag atccatgtat
    14041 gtggaacacc gatcgtaaca gctgcttctt tacatatgga agcagcactt cctaattttg
    14101 taatccatga atataacgtg aatacagaaa tgccacagat gacatcactt gcgctgcatc
    14161 attacgagcc ggtaaacgga ttatttacag tgccggaact tccaggaatt ggaaatgaga
    14221 tcgcaccgga agcatttaag cgtagccagg tagttacgat cgaataaaag aatattttcc
    14281 gggggctgct gtgtcagccc tttttatatt gacagtaact atgaaaatta atagtgacta
    14341 ataaaataat cgctaacttg taatagagga gaaatgcatg taaacttgag tacatgaagg
    14401 aatccgggag gatatcgtat acacaggagg aacacacatc atagaaatta cttttttagg
    14461 cgtggtattt gcggttatta tagttctttt agccataggc agaccgctgt atcaggcgat
    14521 tgcaggcgga atgatcgtaa cagcagggct gtttcatatt ccggtaacag taattgtgac
    14581 taaaatagcc agtgttatta tcaactggtc atctttatct gttttattgt ctttgtatct
    14641 gatcacattt ttacagagga tattagagtc acgcagccag attcgtctgg cacaacagga
    14701 tctgaacggt atttttcata accgccgtat caatacagca ggagcagctt ttttcattgg
    14761 acttctgcca tctgcggctt ccatgatcct ttgtgcagat attgtaaagg atgcgacaga
    14821 gggatattta gatccaaaag agcaggcttt tacagccagc tggttcaggc acataccgga
    14881 aagtgtgctg cctacttata cagcagtact tcttatgtca aatctttctg gagtggaaat
    14941 atcagaattt attgtatata tgatcgtacc agtcattgca ctggcaggac tgggatatgc
    15001 ggtttatctg caccgcatcc ccaatgatac cggcacaccg gcaagcacca accgactggc
    15061 agactttgcc catttgatcc agcatttgtg gtcactgctt cttatcctta tattgatatt
    15121 agtttttcat tttcaggttg taacgtctgt tcttgtatcc attgcacttt gtattatcat
    15181 ttatcgggta acactggatg aaatcaggaa atttattttt gctgcttttg aaaagaagat
    15241 gttgggaaat acctttcttg tattatgtct gaaagaactg atcgctcata caggtgtgtt
    15301 attgctgctg ccggatgcta tggcaaaact gcctctgcct acctatctga tttttggtct
    15361 gctatttttt acagcaactg tcatcagtgg ttctacaggt gccattgcta tgggaacacc
    15421 gctggctttt gcggcgattc cgggaggagc ccccttaatg gcatacctta tgtgcatggc
    15481 ccatgcagcc agccagatct cacctaccca tgtatgtctg gtggtggcag ccgactattt
    15541 tcatgtgacg ttaggagata ttgtaaagaa aacactgcct gtttctattg ccttctgcgt
    15601 attaatgacg ctatattata atgtgataaa gctgtaaaaa aagatccggt aagagcaggg
    15661 agaggggaaa aacatatgta taagagaaaa atagaaaaag atatccgctg tccgttagaa
    15721 tatggtctgg aagtatttgg aggaaaatgg aattcccgca ttatctgtgt actggcagcg
    15781 aaggaagtac tgcgttacag tgaacttcgc cgggaaatgg gaaacattac agatgcagtc
    15841 ctggcagctg cattaaaaga actgatcaca aatggcattg taaagcgcca gtcctatgac
    15901 gagattccac caagagtgga atacagtctt acagaaaaag gaaattccgt gatccctatt
    15961 ttgcagagca tctgccgttg ggccggagta ttctataaag aggatagtga aaatgaaatg
    16021 atccagtgta agaggtgtga ttacagataa agttttgttt tatttctggt tctaatgtta
    16081 agtttaacgt tgcacttgag cctcaaaagt tttatactga agtacaagaa gtatatctat
    16141 catataaatc ataacagaga ggaaccagac acatgaacat cacaggaaaa ggtatgaaat
    16201 tcagatggct gaacaatgca ggctttgaaa tggagttatc cagcggaaaa catcttttag
    16261 tagatccatg gttagacgag gcacagatct atcccatcag tatcgatgag atcgaacggg
    16321 cagattatgt tcttctcacc catacacatg acgatcattc agacagcatc ggacggattc
    16381 agaagcgctt cccacaggca gctatttttg tgggggatct gtctgcggaa gcattgtgta
    16441 aggaatatga cctgaatgta gaacgtctgt tccgtatccg tggcggggaa gaatatgaat
    16501 ttgatgatgt gaagatcgaa gtgatctctg ccagacatac agagtccaaa ggcggaaatt
    16561 actgggatag aggttactgc attcagaagg acggaagcag aagagaaact atgtggtatg
    16621 gttccctgga aatgtacaac ttccgcatta ccgatgttaa tggatacaga gccgttgtct
    16681 ggggtggaat gaccacagag gagcagatcc accgcatgga aaaatacagt ggaaatgaga
    16741 tcgcatttat gcatgtcagc ccaaaacagg atcaccagat gtttgcaaga ctggtgcagg
    16801 ctattaatcc caaggcagtt attccacatc actatgatat ctgggaaacc ttgtttgcag
    16861 caaagccaga gttattagca gatatgaaac ttccggcaga taaagtcaat gcagaaggtg
    16921 ttctggatgt gatccgccag aatatcaaag aaaactgtcc ggatgtggca ttctttatcc
    16981 cggagcatca taaatggtat cagtttggct atgggatcat ggaagcataa ggaaaatctg
    17041 gcagacagga tctggaaaat cgtggtatac ttataaatat gtcccgaata tgcgggggaa
    17101 aagaaggagt ataacatgag tacattttgg attctgttag aacagatgga actatttgta
    17161 atttacattc tggcaggtgt gatactggta aaaagcaggt tgtttaacag ggagacctta
    17221 cagcctatat caaaatttgt cttaagaatg ggacttccat tgcttatctt tacgaatatt
    17281 atcaatgggg tagagcgaaa tgttctgctg agttccggtt ctgttctgat gttagcattt
    17341 ctgttttatg ttgccatgtt ttttatcagt atggg
//
LOCUS       DBMDUA010000042        17282 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1919_length_17282_cov_2.743670, whole genome shotgun sequence.
ACCESSION   DBMDUA010000042 DBMDUA010000000
VERSION     DBMDUA010000042.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 17282)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17282)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17282
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1919_length_17282_cov_2.743670"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            177..1199
                     /locus_tag="ACZPCL_05090"
     CDS             177..1199
                     /locus_tag="ACZPCL_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1161886.1"
                     /translation="MTEFLPGIYKVLQKTDRGNTTEINIFIIPGKKGERSLMIDAGFQ
                     DENCLAQMEGVLKELDISYEDLDVFLTHKHHDHSGLASVYADRGATIYMNPLEERHHY
                     DCLFYSTKKTDPQEQDKVLHSVGVTEEGTPEVWDMFERVRKVVENHNGWTFEVQDFPY
                     KPIAPGAVLHYGDYTFETIDLKGHTFGQLGLFDKEHKIFFCADQVIDGIVPIVATTYP
                     DEHLLRDYFVSLERFHHEFKDCLLLPAHGKEISDAKKVIDRIVFSYLDKVEIMHNILE
                     HSRKARTIREIATIVYGMPHPPKDTAEFIKLKMIMSKCFSCLEYLRDEDFAVREEKDG
                     TFYWRP"
     regulatory      1293..1404
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            1469..2023
                     /locus_tag="ACZPCL_05095"
     CDS             1469..2023
                     /locus_tag="ACZPCL_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HIR1161887.1"
                     /translation="MERLATLFTGSFRQLKQVKTVTVCAMLGAVAIVLGSLSIELGST
                     LRIGFSGIPNEIVHMLFGPVVGSVFAGTMDILKYLIKPTGAFFPGFTLIAMLAGLIYG
                     SFYYKKELNFWRVLAAHITVALICNVLLNTWCLSILYGKAFAVLLPARLVKNAIMCPI
                     DAFIFYHIAKVLDVTGVFKMIRKE"
     gene            2132..3706
                     /locus_tag="ACZPCL_05100"
     CDS             2132..3706
                     /locus_tag="ACZPCL_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR1161888.1"
                     /translation="MDFHISQFLSPGRKEFVKGTLLLTGAGLFCRVLGFFYRIYMSRT
                     IGAEGLGLYNMIHPLFSIAFAVCAGSIQTALSQYIAANQEKGPAVFKCGLFLSLSLSV
                     IPLLLLWKGKMFLAGNILSEPQAARYLPVLAVSVPFACLHACINGYYYGMNKAHIPSF
                     SQIAEQVIRMGAVWLLVGYLEKNGKSVTVGLAVEGHLIGEMASAAFTFLALFLVPPQR
                     KERQNETLIGETGRQRREQKRVEKENVLGDNAVFANLGPILILALPLMGNRLVLNLLG
                     SAEAILIPSRLIDYGLTNKEALSIYGVLTSMALPFILFPSALTNSMAVLLLPSAAKAQ
                     AEKDESRISTMVTLSFRYSCYMGILCIGLFTRFGKSLGTIIFHNELSGSFIMVLCWLC
                     PFMYLATTMGSIQNGLGKTGITFLQNMFAMGIRLVFVLFAVPLFGIRGYLWGFLVSEL
                     LLAFLCCFSVRRQVEFHCNMADCVLKPGLVLLISLGLELALCTAIPVTGSFLEIVFHM
                     GIITVCYGAFLGVILR"
     gene            3841..5028
                     /gene="nifS"
                     /locus_tag="ACZPCL_05105"
     CDS             3841..5028
                     /gene="nifS"
                     /locus_tag="ACZPCL_05105"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HIR1161889.1"
                     /translation="MEKLIYLDNAATTKTRPEVVEAMLPYFTEYYGNPSSVYTFSSES
                     KKAVTNAREIIAKSLGAKTNEIYFTAGGSESDNWALVATAEAYSAKGKHIITSKIEHH
                     AILHTCQYLESRGYEVTYLDVDENGVVKLDELKKAIRPDTILISIMFANNEIGTIEPI
                     KEIGEIAKEHGILFHTDAVQAYAHVPINVDECHIDMLSSSGHKLNGPKGIGFLYIRTG
                     VKIRSFIHGGAQERKRRAGTENVPGIVGYGKAAEIAMANMDERIAKETQLRDYLIKRV
                     TEEVPFTRVNGSRTNRLPNNVNFAFQFIEGESLLIKLDMAGICGSSGSACTSGSLDPS
                     HVLLAIGLPHEIAHGSLRLTLSEENTMEEMDYVVEQIKEIVSYLRGMSPLYEDYMRRT
                     KEK"
     gene            5067..5504
                     /gene="nifU"
                     /locus_tag="ACZPCL_05110"
     CDS             5067..5504
                     /gene="nifU"
                     /locus_tag="ACZPCL_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HIR1161890.1"
                     /translation="MYSEKVMDHFQHPRNVGEIEDASGVGTVGNPKCGDIMRIYLDID
                     DNQIIRDVKFKTFGCGAAVATSSMATELVKGKTVQEAMEVTNKAVMEALDGLPPVKVH
                     CSLLAEEAIHAALWDYAQKNGIKIEGLEQPKENIGEEEEEEEY"
     gene            5501..6577
                     /gene="mnmA"
                     /locus_tag="ACZPCL_05115"
     CDS             5501..6577
                     /gene="mnmA"
                     /locus_tag="ACZPCL_05115"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR1161891.1"
                     /translation="MSRKVVVGMSGGVDSSVAAWLLKEQGYDVIGVTMQIWQDEDTLV
                     QEAEGGCCGLSAVDDARRVAMDLGIPYYVMNFKNEFRTQVMDYFAAEYMAGRTPNPCI
                     ACNRYVKWESLLRRSLSIGADYIATGHYARIEQLPNGRYAIKKSVTAAKDQTYALYNL
                     TQEQLSHTLMPVGNYHKDEIRDMAQKLGLPVASKPDSQEICFIPDHDYASFIEEYTGK
                     TMEPGNFVDLDGNVLGRHKGISHYTVGQRKGLNLAMGYPVFVVAIRPETNEVVLGNGQ
                     DVFTDVVRCNKLNWMALEGVGEEEISVNAKIRYSHKGAPCKIRRIGEDMVECRFEEPV
                     RAATPGQAVVFYQDDYVAGGGTIL"
     gene            6574..7878
                     /locus_tag="ACZPCL_05120"
     CDS             6574..7878
                     /locus_tag="ACZPCL_05120"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR1161892.1"
                     /translation="MRRREKVSVKAAAIILGAALLTAGCSGYQSSEELRKNAENVQAE
                     IVPGEENETENGSDEAIGEKAERENTEASVKQGSNPTAEIIQSETERTTAPAPEFKAV
                     SDTVYVKTNGGSVRVRSSCDTSISDNIVANVGNTTELTRTGTSEKWDRVEYEGKTVYI
                     SSDFVTTEVPKEPETSAASSNAAADGQVTLNSSWKYADFAKIKSGAAVLYRSTASEKK
                     NKVIAVNAGHGTKGGTSVKTQCHPDGTAKVTGGTTGAGATSAVAVSTGMTFSDGTAES
                     KVTLAMAKVLKDKLLAAGYDVLMIRDGEDVQLDNIARSVIANNAADCHIALHWDSTTS
                     NKGAFYMSVPDVASYKNMEPVKSHWQQHNALGASLVEGLKSAGVKIFSGGSMAMDLTQ
                     TSYSTVPSVDIELGDKASDHSQSTLNQLGDGLVAGVKAYFGQ"
     gene            7971..8792
                     /locus_tag="ACZPCL_05125"
     CDS             7971..8792
                     /locus_tag="ACZPCL_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6612 family protein"
                     /protein_id="HIR1161893.1"
                     /translation="MRKVKRFAAIVLTVALLSPTTAYAGNVEDAKALYEQVTEKQKSV
                     TDMNAYYDYQIHLSGSALEGVEDLSNMRLEMNVRMNHLTDMGNLQYSSYSRATVPGQE
                     PMISTMYYKDGTCYMETAGTKIKYPMDLTQMAEQISSQTMMLASGQAQEDLMTDFRLW
                     TEGEDQVIGFVIQGSKMTDYMDQLFGSLGMGSALLSESGITMKFSDIPCEYVVDPNGD
                     CIKMRMKMNMEMGVEGKNIAIDMDGDVGIADPGSPVEINFPNLSEYKDMSEAFAR"
     gene            8909..9736
                     /locus_tag="ACZPCL_05130"
     CDS             8909..9736
                     /locus_tag="ACZPCL_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HIR1161894.1"
                     /translation="MSETPNTITAIKKELLMRLGHDADIFALISLCTREPYVVCDEET
                     FDDEVFLFLDEEEAKKEAAKLAEEKVPVTVAKINGKDMLVFFTGLYTLGVNALVVTDK
                     GEKASMQISDIVKRKSPEDMPQGTVWVENPALHLTAAYLAQEMRRPAASADKERMAEL
                     NEELTADFKKGTFIFALQKEEKGIPLVKLKDGQLYQPAFTDILEFQKFNRENKMRPVV
                     VEAAKVPTVLANEAQGIILNVMGVNLPLTVSRPKVQAAEPKRTIPVPVEEINKALEK"
     gene            9850..12747
                     /locus_tag="ACZPCL_05135"
     CDS             9850..12747
                     /locus_tag="ACZPCL_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR1161895.1"
                     /translation="MGGQEPVKTEKYFSTGLLAHVDAGKTTLSEALLYLTGAIRKMGR
                     VDDRDAFLDTYELERERGITIFSKQAIFDLGDTHVTLLDTPGHVDFSAEMERTIQVLD
                     AAILVVSGADGVQGHTRTVWRLLKRHHIPVFLFVNKMDQSGCDAAALMKDLKAGLDEN
                     CVDFGGWSGEKGEWDPEKEDTDTFYQELAVCDEAVMEAYFENGSIGSDEIGRLVRERK
                     VFPCYFGSALKLKGVEELLAGFQRYGMAKGERKTEKDSPFGAKVFKISRDSQGNRLTH
                     LKVTSGVLKVKDAWGEEEKVNQIRIYSGAKFESVKEAPAGCICAVTGPIHTHPGQGIG
                     SEPASELPMLEPVLTYRILLPFDSDVHKCLQQFKQLEEEEPELHVLWNEELGEIQVQL
                     MGDVQIEILKHMLEERYGLLVGFDTGNIVYKETILNTVEGVGHFEPLRHYAEVHLLLE
                     PGEAGSGLQFDTVCSEDVLERNWQRLILTHLEEKIHRGVLTGAEITDMKVTLLTGRAH
                     MKHTEGGDFRQATYRALRQGLMQAESQLLEPYYGFTLELPSENLGRAMADMDRMFGTF
                     EPPVTTEDGVLLTGKAPVACLRDYQREVIAYTKGRGHLSLWFKDYEPCHNAEEVIEAA
                     GYDPEADLENTADSVFCSHGAGYLVPWYQVKEYMHVESPLEKLGRRANGENDTDGGMQ
                     GDMGRFAGDAGIGSGANGSSSGAGGGRPVSGSAVSTSGSSTSASAAGKRQGGSGPGTS
                     WSDDKELEEIFNRRFGAGKWRSRPDTQSSPTAPRTRNYSANKPLKANETKKPEEEEYL
                     LVDGYNIIFAWEELRELSKVTIDGARQKLMDILCNYQGYKKCTLILVFDAYKVTGNTG
                     EAIDYHNIHVVYTKEAETADQYIEKLAHKIGRQYNVTVATSDGLEQLIIYGQGCHLMS
                     ARDLKEEVAWANEQIREEKAKLEKSGKNYLFHGADEELAAYLEDVRLGRRE"
     gene            12750..13886
                     /gene="fliB"
                     /locus_tag="ACZPCL_05140"
     CDS             12750..13886
                     /gene="fliB"
                     /locus_tag="ACZPCL_05140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin lysine-N-methylase"
                     /protein_id="HIR1161896.1"
                     /translation="MLYTYPHYYRKFQCIASECEDTCCAGWEIMIDDKALEKYKKAKG
                     PVGNRLKNSINWKEGSFLQYHHRCAFLNDENLCDLYTEMGPDSLCRTCRMYPRHVEEF
                     EGSREYSLCLSCMEAAKIILGCEEKVQFLTKEDEKEETYEDFDFFLYTKLMDARDLIF
                     KILQDRSLDMRLRMAEVLALAHDLQRRVRDNVLYQTDALFEKYNSSRRDGYFKEKLAG
                     ITGYSRYEVIKAIMDLLEKMEPLNHQWPEYRDELKVCLLGDGEAAYEEMRKAFFQRPE
                     AEKMNLWTEQLMVYFVYTYFCGGVYNENPYGKMKAAIVSTIMIQDMMMVHWKKHGTLT
                     LKEAADVAHRYSREIEHSDENKGLLEETLTREEEFRLRALLAAL"
     gene            14258..15094
                     /locus_tag="ACZPCL_05145"
     CDS             14258..15094
                     /locus_tag="ACZPCL_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR1161897.1"
                     /translation="MSECSHNCGTCGENCSSRTQDKKSFLEALAPESSVKKVIGVVSG
                     KGGVGKSLVTSMMAVSMSRKGKHAAILDADITGPSIPMAFGVAHEKAGVSEDGKLMIP
                     ARSLEGVDIMSANLLLDHDTDPVIWRGPVIAGAVKQFWTETLWKDVDYMFVDMPPGTG
                     DVPLTVFQSLPVDGIIIVTSPQELVGMIVAKAVNMAKKMNVPILGIVENMSYLECPDC
                     GKHINVFGESHTEQVAEEYGLKLLAQIPIDPKIAQMVDGGQVEYIEMPWFEEAVKAVE
                     NL"
     gene            15119..15832
                     /locus_tag="ACZPCL_05150"
     CDS             15119..15832
                     /locus_tag="ACZPCL_05150"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIR1161898.1"
                     /translation="MNINLNPNSELNQSIVNVPDVVQVPDVWINEARQFRMAMMMYAC
                     AIREVKTKLEVLNDELSIKNQRNPIEMIKSRVKKPMSILEKLQRRGLPVSVESMVKNL
                     DDVAGIRIICSFVDDIYEVAEMLVRQDDVTVIAIKDYIKNPKPNGYRSYHMIIEIPVF
                     FSDSKKPIRVEVQIRTIAMDFWASLDHQLKYKKSFVDLNGEISGELKQCADVIAQTDN
                     KMLEIRKRIEAQGVTVSRD"
     gene            complement(15829..16674)
                     /locus_tag="ACZPCL_05155"
     CDS             complement(15829..16674)
                     /locus_tag="ACZPCL_05155"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1161899.1"
                     /translation="MDIFFKDNLNSSDQLYIEQLLCASMAASTDNTGSWGITPALTDI
                     LDADGWWFAASDPDLISKSCPVSKTYLSGCMAVYQTGDDSFECVPFVHPDLRKKGIFS
                     SILAAACEEDQIPGEGSVYFSCGTENKLWKNVLEHIGAEHAWDEYMMERELTGFETMA
                     NEKTADMLYCRILENTSSTAWQAVGSRSLLTQNTAENLTAPEVTCFLAPQGNSVYLYS
                     LEVLPELRNKHLGTAFLSVLLPQLAKKGFKTIKLQVSSENLPAMALYKKTGFCITQTL
                     SFYEY"
     gene            16892..17254
                     /locus_tag="ACZPCL_05160"
     CDS             16892..17254
                     /locus_tag="ACZPCL_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HIR1161900.1"
                     /translation="MKYVRQIGIILGITLAGEFLNQILPLPVPAGVYGLFLMLAALMT
                     GVVKLESVEGTGNFLMDTMSMMFVPATVGILECADELKAVLIPFLIIIAVSTVVVMIV
                     TGKVAQFMMESAEKKKEK"
BASE COUNT         5105 a         3393 c         4420 g         4364 t
ORIGIN      
        1 aaaggcacga cctgccaacg atgcgcactc gcgcgatgag gaagtttgtc gcaagcgacc
       61 gcgctcggcg cgagaatgtg ccaaggcaca ttcttattta catatggatt tacaaaaata
      121 aatgaatgtt ctatacttta taaaagggta gtacgcaagg aggaaggtta aagattatga
      181 cagaatttct gccgggtatt tataaggttc ttcagaaaac agatcgtggc aataccacgg
      241 agatcaatat atttattatt cctggaaaaa aaggggaaag aagcctgatg atcgatgccg
      301 ggtttcagga tgaaaactgt ctggcacaga tggagggagt attaaaagag ctggatattt
      361 cctatgaaga tctggatgta tttttaaccc ataaacatca tgaccattca ggactggcaa
      421 gtgtgtatgc agaccgtggt gctaccattt atatgaaccc gttagaggaa cgccaccatt
      481 atgactgcct gttttacagc accaagaaaa cggatcccca agaacaggat aaagtccttc
      541 acagtgtagg agttacagaa gagggtacac cggaagtctg ggatatgttt gaacgggtga
      601 gaaaagtagt ggaaaatcac aatggctgga cctttgaggt gcaggatttc ccttataagc
      661 ccatagcacc gggagctgta ctgcattacg gtgactacac atttgaaaca atagatctga
      721 aaggacatac ctttggacag ctgggacttt tcgacaaaga gcataagata tttttctgtg
      781 cagaccaggt gatcgatggg atcgttccta ttgttgcaac aacgtatccg gatgaacatc
      841 ttttaaggga ttattttgtt tctttggagc gtttccacca cgaatttaag gactgtctgc
      901 tccttccggc acatggaaag gaaatttctg atgcaaaaaa agtcatagac cgtattgtat
      961 tttcctatct tgacaaggta gagatcatgc ataatatcct ggaacattcc agaaaagcaa
     1021 ggacgatccg ggagatcgca accattgtct acggaatgcc tcatcctccg aaagatacag
     1081 cagaatttat taaattaaag atgatcatga gcaagtgttt ctcctgcctg gaatatttaa
     1141 gggatgaaga ttttgcggtc cgtgaggaaa aagacgggac cttttactgg cggccataat
     1201 ttctttcaaa tacaatgtcc gtgaaaaagt acttgcaatt ccataaaaaa agagtatagt
     1261 tacttaaaat agctatcaaa acaacataag aaagcagagt aggtttatgt gcgttaagtg
     1321 ccggctgaac agggagttgt cagtcagacg aaaggaattt tgaaagaaaa ggttcctgcg
     1381 gtacataagc cgcatcccgc tgcatcagag aaaatcttga ttatctccga ttgtagtatg
     1441 aggtatctgc aaaggaggta atttttttat ggaaagatta gcaactttgt ttaccggttc
     1501 cttcagacag ttaaaacagg taaaaacggt tacggtatgt gccatgcttg gggctgttgc
     1561 cattgttctc ggttcattaa gcatcgagct tggaagtacc ctgcgtatcg gtttttccgg
     1621 tattcccaat gagatcgtcc acatgctgtt cggtcctgta gtgggaagcg tttttgcagg
     1681 cactatggat attttaaaat atctgatcaa gcctacggga gcattttttc caggctttac
     1741 gctcatagcc atgcttgcag gcctgatcta cggcagcttt tactataaga aggaattaaa
     1801 tttctggaga gtgcttgcag cccatattac agtggctctg atctgcaatg tgctccttaa
     1861 tacctggtgt ctgtccattc tttacggaaa ggcatttgca gtgctccttc ctgcaagact
     1921 ggtaaagaat gcgatcatgt gtcctatcga tgcttttatt ttttatcata ttgccaaagt
     1981 tttagatgta acaggtgtat ttaagatgat cagaaaagaa taagatctta actgaaaata
     2041 acttttgcga aaagacctga ggtttgaata cttccggtct ttttttcata cattgaaata
     2101 acgtacattg gggcagtaag gagagccggc tatggacttt catataagcc agtttctttc
     2161 accggggaga aaagaatttg taaaagggac gctgcttctt acaggggccg ggcttttctg
     2221 ccgtgtttta ggcttttttt accggatcta catgtcacgt accatagggg cagaaggtct
     2281 gggactttac aatatgatcc acccgctgtt cagtattgcc tttgcggttt gcgccggttc
     2341 catccagacg gctctgtccc agtacattgc cgccaaccag gaaaaaggac cggctgtttt
     2401 taaatgcggt ctgttcctgt ctctaagtct ttctgtgatc cctcttttgc tgttgtggaa
     2461 aggaaaaatg tttctggctg ggaatatcct ttcggaaccc caggcagccc gttatcttcc
     2521 ggtactggca gtttccgtac catttgcctg tctccatgcc tgcatcaacg gctattatta
     2581 tggaatgaac aaggcacata ttccgtcttt ttcacagatc gcagagcagg tgatccgtat
     2641 gggtgcagtg tggctgttgg tggggtatct ggaaaaaaat ggaaaatcgg ttacagtagg
     2701 tctggcagta gaaggccatc tgatcggaga aatggcatcg gcagctttta catttctggc
     2761 gctgtttctg gtgccgccgc agaggaaaga gagacaaaat gaaacactga taggggaaac
     2821 aggcaggcag aggcgggagc agaagcgtgt tgagaaagaa aatgtccttg gagataacgc
     2881 ggtatttgca aatctgggtc cgatcttaat tttggcactt cctcttatgg gcaaccgtct
     2941 ggtgttaaat cttctcggaa gtgcggaagc tatcctgatc cccagccgcc tgatcgatta
     3001 tggtctgacc aacaaagaag cattatccat atatggagta cttaccagca tggcgcttcc
     3061 ctttatcctg tttccttcgg ctcttacgaa ctccatggca gtacttttac ttccatcggc
     3121 tgccaaggca caggcggaaa aagacgaaag ccgcatttct accatggtca cactgtcttt
     3181 ccggtacagc tgttacatgg gtatcctgtg catcggtctt tttacccggt ttggcaaaag
     3241 ccttggcacg atcattttcc ataatgagct gtccggctct tttattatgg tactttgctg
     3301 gctctgtccg tttatgtatc tggcaactac tatgggaagc atccaaaatg gccttggaaa
     3361 aacgggtatt accttcctgc aaaatatgtt tgccatgggg atccgtctgg tatttgtgct
     3421 gtttgcagtg ccgctgtttg gtatccgggg atatttatgg ggctttctgg tcagcgaact
     3481 gcttttagcc tttttatgct gtttttctgt ccgccgtcag gtggaatttc actgcaatat
     3541 ggcagactgc gttttaaagc cggggctggt gcttttgatc agtctggggc ttgaactggc
     3601 cttgtgtaca gctattcctg taacaggcag ttttctggaa atagtattcc atatgggtat
     3661 aataaccgtc tgttatgggg catttttagg ggtgatcctc agataaaggg gagataaacc
     3721 aactaacttt gtatgaaatt aaaacaatcg ggtattcaca ttgagtttca tggaatacta
     3781 tgttatagta aagggtagcg ataaagcaat tttttataga agaaaggcgg aaatattatt
     3841 atggaaaagc tgatttatct tgataatgca gccactacca aaacaaggcc ggaggttgtg
     3901 gaagcaatgc ttccttattt tacagaatat tatggaaatc cctcttctgt atatacattt
     3961 tcatcagaaa gcaaaaaagc agttaccaat gcaagagaga tcattgcaaa gtctttaggc
     4021 gcaaagacaa acgagatcta ctttacagca ggcggaagtg agtctgacaa ctgggcatta
     4081 gttgctacag cagaggctta cagcgcaaag ggcaagcaca tcatcaccag caagatcgag
     4141 catcatgcca tccttcatac ctgccagtat ctggagagca gaggttatga ggtaacttat
     4201 ctggatgtag atgaaaatgg tgttgttaaa ttagacgaat taaagaaagc gatccgtccg
     4261 gatactattc ttatttctat tatgttcgca aacaatgaga tcggaaccat tgagccaatt
     4321 aaggagatcg gtgagatcgc aaaagaacac ggcattttat tccatacaga cgctgtacag
     4381 gcttatgccc atgtgccgat caatgtagat gagtgccaca ttgatatgtt aagctccagc
     4441 ggccacaagt taaacggacc aaagggaatt ggtttcctgt atatccgtac cggtgtaaag
     4501 atccgttcct ttatccatgg cggagctcag gagcgtaagc gccgtgcagg tactgaaaat
     4561 gttcctggca tcgtaggtta cggaaaagca gctgagatcg ctatggcaaa catggatgag
     4621 cgtattgcaa aagagactca gttaagagat tatctgatca aacgtgtaac agaagaggtt
     4681 ccatttacca gagttaacgg ttccagaacc aatcgtctgc caaacaacgt aaactttgca
     4741 ttccagttta tcgaaggcga gtctttactg atcaagttag acatggcagg tatctgcggt
     4801 tccagcggtt ctgcatgtac ttccggatcc ttagacccat ctcatgtact gttagccatc
     4861 ggtctgcctc atgagatcgc acatggttcc ctgcgtctga ccttaagcga agagaacaca
     4921 atggaagaaa tggattatgt agtggagcag atcaaggaga tcgtttctta cttaagaggt
     4981 atgtctcctt tatacgaaga ttatatgaga cgcacaaaag aaaaataagt ataggtaaat
     5041 aaccaaccat caggaggaat tttttcatgt attcagaaaa agtaatggac cattttcagc
     5101 atccaagaaa tgtgggagaa atagaagacg ccagcggcgt aggaactgta ggaaacccaa
     5161 aatgtggtga catcatgcgt atctacttag atatcgatga caaccagatc atccgtgacg
     5221 taaagtttaa gactttcggc tgcggcgctg cagttgcaac cagcagtatg gctactgaat
     5281 tagtaaaagg aaagactgtt caggaagcaa tggaagttac caacaaggca gttatggaag
     5341 cattagatgg acttccaccg gtaaaggtac actgctccct gttagcagaa gaagccatcc
     5401 acgcagcttt atgggactat gctcagaaaa acggtattaa gatcgaagga ttagagcagc
     5461 ctaaggagaa catcggcgaa gaggaagaag aggaagagta ttgagtagaa aagtagtagt
     5521 aggcatgtca ggaggagttg actcctccgt ggcagcctgg ctgttaaaag aacagggtta
     5581 cgacgtcatc ggcgtgacca tgcagatatg gcaggatgag gataccctgg ttcaggaagc
     5641 agaggggggc tgctgcggct taagcgctgt agatgatgcc cgccgtgttg ctatggactt
     5701 gggtatccct tattatgtca tgaattttaa aaatgagttc cgcacccagg tcatggatta
     5761 ttttgctgcg gaatatatgg caggacgtac cccaaatcca tgtatcgcct gcaaccgtta
     5821 tgtaaaatgg gagtctctgc ttcgccgcag tctttccatt ggagcagatt acattgctac
     5881 cggacattat gcaaggatcg aacagcttcc aaacggcaga tatgccatta aaaagtcagt
     5941 aactgcagca aaggaccaga cttatgccct ttataacctg acccaggagc agctttcaca
     6001 taccttaatg ccggtaggaa actatcacaa agacgagatc cgcgatatgg cgcaaaaact
     6061 gggacttccg gtagcttcca agccggacag ccaggaaatc tgctttatcc cggaccatga
     6121 ctatgcatcc tttattgaag aatacacagg aaagaccatg gaaccaggca atttcgtaga
     6181 tttagatgga aatgtgctgg gacgccataa aggtatctcc cattacaccg taggccagcg
     6241 aaaaggttta aaccttgcta tgggatatcc ggtctttgta gtagctatcc gtccggagac
     6301 aaatgaagtt gtactgggaa atggccagga tgtatttaca gatgtagtcc gctgcaacaa
     6361 gttaaactgg atggcattag aaggtgttgg agaggaagag atttctgtca atgccaagat
     6421 ccgctacagt cacaaaggcg caccatgtaa gatccgccgg atcggagaag acatggtaga
     6481 atgccgtttt gaagagccgg tacgtgctgc aacacctgga caggcagttg tattctacca
     6541 ggatgattat gtagcaggcg gaggcacgat cttatgagaa gaagagaaaa agtcagtgta
     6601 aaggcagctg ccattatcct tggagcagcg cttttgacag caggctgttc cgggtatcag
     6661 tccagcgaag aactgaggaa aaatgcggaa aacgttcaag ctgaaattgt gcctggtgag
     6721 gaaaacgaaa ctgaaaatgg ttctgacgaa gcaatcggcg aaaaagcaga aagagaaaac
     6781 acggaagcat ctgttaaaca gggcagcaat cccaccgcag agatcatcca aagcgaaacg
     6841 gaacgcacca cagccccggc gccggaattt aaagcagttt ccgatacggt ctatgtaaag
     6901 accaacggcg gaagcgttcg tgtccgttct tcctgtgata cttcaatcag tgataacatt
     6961 gtagcaaatg ttggaaatac aacagaactt acccgtaccg gtaccagcga aaaatgggac
     7021 agagtggaat atgaaggaaa gaccgtctat atttcttctg actttgtgac cacagaagtt
     7081 ccaaaggagc cggagacaag cgcagcttct tctaatgcag ctgcagatgg acaggtgaca
     7141 ttaaattctt cctggaaata cgcagatttt gccaaaataa aatccggggc agcagtcctt
     7201 taccgcagta cagcttctga aaagaagaat aaggtgatcg cagtgaatgc gggtcacggc
     7261 acaaaagggg gaacttctgt aaagacccag tgtcatccgg atggaacagc caaagttaca
     7321 gggggtacta caggtgcagg tgctacttct gcagtagctg tttccacagg aatgacattt
     7381 tctgacggaa cagcagagag caaggtaaca ctggctatgg caaaagtact gaaagacaag
     7441 cttttggcag cgggctatga tgtactgatg atccgtgacg gggaagatgt gcagttagac
     7501 aacattgcaa gaagtgtgat tgccaataac gcagcagact gccatattgc tcttcactgg
     7561 gactctacaa ccagcaacaa aggcgcattt tacatgagcg ttccagatgt ggcttcctat
     7621 aagaacatgg aaccggtcaa gtcccactgg cagcagcaca acgcactggg agcaagcctg
     7681 gtagaaggcc ttaagagcgc aggtgtgaag atcttttccg gcggttctat ggcaatggat
     7741 ctgacacaga cttcttattc taccgtacct tctgttgata ttgagctggg agacaaggcg
     7801 tctgatcatt cacagagcac cttaaaccag ttaggagatg gcctggtagc aggtgtaaaa
     7861 gcttatttcg gacagtagta tgtaacttcc cgcatcctgt gcgggaatgg tgtagttgct
     7921 ttgcatgggg aatgtaaagt taacgctata tagcagaaag gagttacgtt atgagaaagg
     7981 taaaacgttt tgcagcaatc gtccttacag tagcactttt aagtcctacg actgcctatg
     8041 caggcaatgt ggaagatgcc aaagctcttt atgagcaggt aacagaaaag cagaaatccg
     8101 ttacggatat gaatgcttat tatgattatc agatccatct ttccggcagt gccttagaag
     8161 gcgtggaaga tctcagcaat atgcgtctgg aaatgaatgt gcggatgaac catttaacag
     8221 acatgggaaa tctgcagtac agcagttaca gccgtgccac agtacctgga caggaaccga
     8281 tgatcagtac catgtattat aaagatggta cctgctacat ggaaactgcc ggaacgaaga
     8341 tcaaatatcc tatggatctg acacagatgg cggaacagat cagcagtcag acaatgatgc
     8401 tggcttcagg acaggcgcag gaagatctga tgacagattt ccgcctgtgg acagagggag
     8461 aagaccaggt aattggtttt gtgatccagg gaagcaagat gaccgattat atggatcagt
     8521 tattcggcag tctgggaatg ggaagtgccc tgctttctga aagcggcatc accatgaaat
     8581 tttcagatat cccatgtgaa tatgtagtag atccaaacgg tgactgcatt aaaatgcgta
     8641 tgaagatgaa catggaaatg ggagtagaag gaaagaatat tgccattgac atggacgggg
     8701 atgtaggaat tgcagatcca ggcagtcctg tagagatcaa tttcccgaat ttaagtgaat
     8761 ataaggatat gtcagaggct tttgcaagat aaacaggcct ggtgattgcg tcctttcggg
     8821 gaatgtggta caatcatacc caatgaagca gatgtttttc tgaaaattgc aggaaaacat
     8881 taaagtagca aaggagatta aaactggaat gagtgagaca ccgaatacaa ttacggccat
     8941 taagaaggaa cttcttatgc gccttggaca tgatgcagat attttcgccc tgatctctct
     9001 gtgtacaaga gagccatacg tagtatgtga tgaggagacc tttgatgatg aggtattctt
     9061 attcctggac gaagaagaag caaaaaaaga agctgcaaaa ctggcagagg agaaagtacc
     9121 tgttacagtt gccaagatca atggaaaaga tatgctggta ttctttaccg gtctgtatac
     9181 actgggtgta aatgccctgg tggtaactga caagggagag aaggcttcca tgcagataag
     9241 cgacattgta aagcgcaaat ccccggaaga tatgccacag ggaactgtat gggtagaaaa
     9301 tccggcctta catctgacag ctgcttatct ggcacaggaa atgcgccgtc cggctgcatc
     9361 tgcagataag gaaagaatgg ctgaattaaa cgaagagctg actgctgact ttaagaaggg
     9421 aacctttatt tttgccctgc agaaagaaga aaagggtatt ccgttagtaa agttaaaaga
     9481 cggacagctg tatcagcctg catttactga tattttagag ttccagaaat ttaaccgtga
     9541 gaacaagatg cgcccggttg tagtagaagc tgccaaagta ccaactgtac tggcaaatga
     9601 agcacagggc attatcttaa atgtaatggg tgtaaacctg cctttaactg tttccagacc
     9661 aaaggtacag gctgcagaac ctaagcgtac cattccggtt ccggtagaag agatcaacaa
     9721 agcacttgaa aaataaattc aggtttttgt ttcattgatg aatgttcagc caaaaaggga
     9781 ctgccgtata ttcttgtatg cggcagtcct ttatgactat aggagtttta ctgtttcagg
     9841 aggtgtttta tgggcggaca ggagccggtt aaaacagaaa aatatttcag cacaggtctg
     9901 ctggcccacg tagatgcagg aaagaccaca ctttcagagg cacttttata tcttaccggt
     9961 gccatacgga aaatgggaag agtggacgac cgggatgcat ttcttgatac ctatgaatta
    10021 gagagagaac gcggtattac cattttttca aaacaggcca tatttgacct tggggatacc
    10081 catgtaaccc tgcttgatac accgggacat gtagattttt ccgcggaaat ggagcgtacc
    10141 attcaggttc tggatgcagc gatcctggta gtaagcggtg cagacggagt acagggacat
    10201 acaagaacgg tatggagatt gttaaaacgt catcacattc ctgtattttt atttgtaaac
    10261 aaaatggatc agtccggctg tgatgcggct gctcttatga aggacctgaa agcaggttta
    10321 gatgaaaact gtgtggattt tggcggctgg tccggtgaaa aaggagaatg ggatcctgaa
    10381 aaagaggata cagatacctt ttatcaggag ctggcagtct gtgacgaggc agtgatggaa
    10441 gcctattttg aaaatggttc cataggatca gatgagatcg gccgtttagt ccgtgaacga
    10501 aaggtatttc cctgctattt tggttctgcc ttaaaattaa agggtgtgga agaactgtta
    10561 gcaggctttc agcgctatgg gatggcaaaa ggggaaagga aaacggaaaa agacagtcct
    10621 tttggagcaa aagtatttaa gatatccaga gacagtcagg gcaaccgcct gacccattta
    10681 aaagtcacct ccggtgtgct gaaggtgaaa gatgcctggg gggaagaaga aaaggtcaac
    10741 cagatccgta tttattccgg ggccaaattt gaaagtgtaa aagaggctcc tgcaggctgt
    10801 atctgtgcag tgaccggccc tatccatacc catccgggac agggaattgg cagtgaaccg
    10861 gcatcagaac tgcctatgct ggaaccggtt cttacctacc gcatcctgct gccgtttgac
    10921 agtgatgtac ataaatgtct gcagcagttt aagcagttag aagaggaaga accggaactt
    10981 catgttctct ggaacgaaga actgggagag atccaggtac agcttatggg agatgtacag
    11041 atcgagatct taaagcatat gctggaagag cggtacggtc tgttggtagg ctttgataca
    11101 ggaaatattg tatacaaaga gaccatttta aatacagtag agggcgtagg gcattttgag
    11161 ccgctgcgtc attatgcaga ggtgcatctg ctgttggaac cgggagaggc cggaagcggg
    11221 ctgcagtttg atacggtgtg cagtgaggat gtattggaac ggaactggca gaggctgatt
    11281 ctgacccatc tggaagagaa gatccacaga ggtgtgctta caggagcgga gatcacagat
    11341 atgaaggtga ctttgctgac agggcgtgcc catatgaagc atacagaggg cggagatttc
    11401 cgtcaggcta cttaccgggc gctgcgccag ggactgatgc aggcggaaag ccagctgctg
    11461 gaaccgtatt atggatttac actggaactt ccctctgaaa atctgggacg ggccatggcg
    11521 gatatggatc ggatgtttgg aacctttgag ccgcctgtga ctacggagga cggtgttctg
    11581 ttaacaggaa aggcgcctgt tgcctgtctg cgtgattacc agagagaggt gatcgcctat
    11641 acaaagggca gaggtcactt aagtctctgg tttaaggatt atgaaccatg tcacaatgca
    11701 gaagaggtga tcgaggcagc cggttatgat ccggaggcag atctggaaaa tacagccgac
    11761 tccgttttct gctcccacgg agccggatat ctggtgccat ggtaccaggt gaaggaatat
    11821 atgcatgtgg aaagtccgct ggagaagctg gggcggcgag caaatgggga gaatgatact
    11881 gacgggggca tgcagggaga tatgggccgg tttgctggtg atgcaggtat tggttcaggt
    11941 gcgaatggtt cttcatctgg tgctggaggc ggcagacctg tgtctggcag tgctgtttca
    12001 acgtctggtt cgtccacatc tgcatcagca gctggaaaac gacagggagg ttccggtcct
    12061 ggcacctcct ggagtgatga caaagaactg gaagagattt tcaaccgccg tttcggtgca
    12121 ggaaaatggc gcagcagacc ggatacacag agtagtccca ctgccccgag gaccagaaat
    12181 tattctgcaa acaaaccctt aaaggcgaat gaaacaaaga agccggaaga ggaagaatat
    12241 ctcctggtag acggctacaa cattattttt gcctgggaag agctgcggga actttccaaa
    12301 gtaaccattg acggagccag acagaaactg atggatattt tatgcaatta tcagggatat
    12361 aaaaaatgta ccctgatcct ggtatttgac gcctataaag tgacaggaaa taccggcgaa
    12421 gccatcgatt accacaatat ccatgtagtg tatacaaaag aagcggaaac tgccgatcag
    12481 tacattgaaa aactggccca taagatcgga cgccagtaca atgtaaccgt agctacttct
    12541 gacggactgg agcagctgat catctatgga cagggctgtc atctgatgtc agccagagac
    12601 ttaaaagaag aggttgcctg ggctaatgag cagatccggg aagaaaaggc aaaactggaa
    12661 aaatctggga aaaattatct gttccacggc gcagatgaag aactggcggc atacttagaa
    12721 gatgtccgtc ttggcaggag agaatagaaa tgctttacac atatccacat tattatagaa
    12781 agtttcagtg cattgcctca gaatgtgagg atacctgctg cgcaggctgg gagatcatga
    12841 tcgatgacaa ggcgctggaa aaatataaaa aggcaaaagg tcctgttgga aacaggctga
    12901 aaaattccat taactggaaa gaaggctctt ttttgcagta tcatcaccgt tgtgcctttt
    12961 taaatgatga aaatttatgc gacctgtata cagaaatggg accggacagc ttatgccgca
    13021 cctgccgcat gtatccccgc catgtagagg aatttgaagg cagcagggag tattctctct
    13081 gtctttcctg tatggaggca gcgaagatca ttcttggctg tgaggaaaag gtgcagttcc
    13141 ttacaaaaga agatgaaaaa gaggaaactt atgaggattt cgactttttc ttatatacca
    13201 agctgatgga tgcaagggac ctgatcttta agatcttaca ggacagaagc ctggatatgc
    13261 ggcttcgcat ggcagaggtt ctggcattag cccatgacct gcaaagaaga gtgcgggaca
    13321 atgtgcttta ccagacagat gctttatttg aaaaatataa tagttccaga agagatggtt
    13381 atttcaaaga aaaactggcg ggaattacag gatacagccg ttatgaagta ataaaagcga
    13441 tcatggatct tttagaaaaa atggaaccgc taaatcacca gtggccggag tacagagatg
    13501 aattaaaagt ctgtctttta ggtgatgggg aagcagctta cgaagagatg agaaaagcct
    13561 ttttccaaag accggaagca gaaaaaatga acctctggac agaacagctg atggtgtact
    13621 ttgtatacac ctacttctgc ggtggcgtat acaatgaaaa tccctatgga aaaatgaaag
    13681 ccgccatcgt atccaccatt atgatccagg atatgatgat ggtccactgg aaaaaacatg
    13741 gtacactgac tttaaaagag gcagcagatg tagcccaccg ttattcccgt gagatcgagc
    13801 attccgatga gaataaggga ctgttggaag agacgttgac ccgcgaagaa gagtttcgtt
    13861 tgagggcact gcttgcggct ttatgaaaat aggtatataa tgagtgaagg gaaaaacaag
    13921 cggctgcgaa gcagacattt gttgccaagc atatcgagga ggcgcctgtg acgcgtccgc
    13981 tgatttttca cgaaccatta acgaatgagt cgtctgcgtc agcagacagt agagcgcgac
    14041 agcgcgggac tcatgagtga acaagagtga agggaaaaac aagcggctgc gaagcagaca
    14101 tttgttgcta agcatatcga ggaggcgcct gtgacgcgtc cgctgatttt tcacgagcca
    14161 ttaacgaatg aaccgtctgc gtcagcagat agtagagcgc gatagtgcgt gttcatgagt
    14221 gaacaaatga gatagaaaat taaaggagaa gatacaaatg agtgaatgtt cccataactg
    14281 tggtacctgc ggcgaaaact gcagcagccg tacccaggat aaaaaaagct ttctggaagc
    14341 gctggcaccg gaaagttctg taaaaaaagt aattggtgtt gtcagcggta aaggtggtgt
    14401 aggtaaatca cttgttacct ccatgatggc agtaagcatg agccgcaagg gcaaacatgc
    14461 agcaatttta gatgcggata ttaccggtcc gtctattcct atggcatttg gcgttgccca
    14521 tgaaaaagca ggtgtttccg aagacggaaa actgatgatc cctgcaagat ccttagaggg
    14581 tgtagatatc atgtctgcca accttctgtt agaccatgat accgatcctg tcatctggag
    14641 aggtcctgtg atcgcaggcg ctgtaaagca gttttggact gagacactgt ggaaagatgt
    14701 agattacatg ttcgtagata tgcctccggg cactggcgat gttccgttga ctgtattcca
    14761 gtcacttcct gtagatggta tcattatcgt tacctctcct caggaactgg taggaatgat
    14821 cgtagccaag gcagtaaata tggcaaagaa gatgaatgtg ccgatacttg gtattgtaga
    14881 aaatatgagc tatctggaat gtcctgactg cggaaagcac attaatgtat ttggtgagag
    14941 ccatacagag caggttgcag aagaatacgg cttaaagctg ttagcccaga tcccgatcga
    15001 tccaaagatc gcccagatgg tagatggcgg tcaggtagaa tatattgaga tgccatggtt
    15061 tgaggaagct gtaaaggcag tggagaacct gtaaacacag gcggaaagtg gtgacacgat
    15121 gaatatcaat cttaatccta acagtgagct gaaccagtcc attgtcaatg tgccggatgt
    15181 ggtccaggtc ccggatgtat ggatcaatga agcaagacag tttaggatgg caatgatgat
    15241 gtatgcctgt gccatccgtg aagtaaagac aaaattagaa gtactcaatg atgaattatc
    15301 cattaaaaac cagagaaacc ccattgaaat gatcaaatcc cgtgtaaaga agcctatgag
    15361 tatcttagag aagcttcagc gccgggggct gccggtatcg gtggagtcta tggtgaaaaa
    15421 tctggatgat gtggcaggta tccgcattat ctgttctttt gtagatgata tttacgaagt
    15481 agcggaaatg ctggtgcgcc aggatgatgt aactgttatt gcgattaagg attatattaa
    15541 aaatccaaag ccaaacggct accgcagtta ccatatgatc attgagatcc cggtattctt
    15601 ctcagacagt aagaagccca tccgcgtaga ggtacagatc cgtaccatcg ctatggactt
    15661 ctgggcaagt ctggatcatc agttaaagta taaaaaatcc tttgtggact taaatggtga
    15721 gatcagcggt gaattaaaac agtgcgctga tgtgatcgcc cagacggaca acaagatgtt
    15781 agagatccga aagaggatcg aagcccaggg tgtgactgta tcaagggatt aatactcata
    15841 aaaagacagg gtttgcgtaa tgcaaaaccc tgtcttttta tacagggcca tggccgggag
    15901 attttctgag gaaacctgaa gctttatggt cttaaagcct ttttttgcca gctggggaag
    15961 gaggacagat aaaaaggcag tgcctaaatg tttatttcta agttccggga gaacttcaag
    16021 gctgtataaa tagacagagt ttccctgggg tgcaagaaaa caggtgactt ccggtgcggt
    16081 cagattttct gcagtattct gtgttagaag gctgcggctg ccaacagcct gccaggctgt
    16141 gctgctagta ttttccaaaa tccggcagta aagcatatct gctgtttttt catttgccat
    16201 tgtttcaaat cccgttaatt cccgctccat catatattca tcccaggcat gctctgcccc
    16261 aatatgttcc aggacatttt tccacagttt attttcagta ccacaggaaa aatacacaga
    16321 gccttctcct ggaatttggt cttcttcaca ggcagcagcc aggatagaag aaaaaattcc
    16381 ctttttacga agatccgggt gcacaaaagg cacacattca aagctgtcat ctcctgtttg
    16441 gtatacagcc atacagccgg acaggtacgt tttagagacc gggcatgatt tagaaatcag
    16501 gtccggatca gaagctgcaa accaccagcc gtcagcatcc agtatatctg taagcgctgg
    16561 cgtgataccc caggatccgg tattatctgt agaggctgcc atggatgcac acaaaagctg
    16621 ttctatatat aattggtctg atgaatttaa gttatcttta aagaaaatgt ccatgaaata
    16681 agctcccaat atagttttta taatgttttt attgtaatcc aatggttttc agggcgcaac
    16741 cactgttaaa tgcccggatt gtattttttg tgtatttccg gcattatctc ttgaatatga
    16801 aaaaacgcag tggtagaata atccagaaaa atttaacgga cgcaaaagaa aaaatattgt
    16861 catccgctaa aacatcagga ggaaaacaaa gatgaaatac gtaagacaga tcggtattat
    16921 cctgggtatc actctggctg gagaattttt aaaccagatc ctgccccttc ctgttccggc
    16981 aggtgtatac ggtctttttc ttatgctggc agctcttatg actggcgtgg taaagctgga
    17041 aagcgtagaa ggaaccggca atttcctgat ggataccatg tctatgatgt ttgtaccggc
    17101 aacagtaggg atcctggagt gcgcagatga gctgaaagca gttcttatcc catttttgat
    17161 cattatcgca gtttccacag tggtagtaat gatcgttacc ggaaaagtgg cacagtttat
    17221 gatggaatca gcagagaaga aaaaggagaa atgatcaaat gagtacatta agagagatcc
    17281 tg
//
LOCUS       DBMDUA010000043        17203 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1927_length_17203_cov_3.290960, whole genome shotgun sequence.
ACCESSION   DBMDUA010000043 DBMDUA010000000
VERSION     DBMDUA010000043.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 17203)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17203)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17203
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1927_length_17203_cov_3.290960"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="ACZPCL_05165"
     CDS             complement(<1..600)
                     /locus_tag="ACZPCL_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012354.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1161901.1"
                     /translation="MNQQQLIFIREIARTGTIRGAAACVDRTPSALTRGLKSVENELG
                     CQLFRRIREGMVPTSEGETVLSYGERILKSLQEIKEKRIWSENEIRYLLCIRENKSIT
                     GAAETLFIAQPSLSQVLKEVEEEVGFKIFQRDRRGAEETKAGAELLEQLEEVWKLWKE
                     MREELGEYRELKKGTVTIGIPMNLVCKISGKEYEKNYNDD"
     gene            complement(845..2155)
                     /locus_tag="ACZPCL_05170"
     CDS             complement(845..2155)
                     /locus_tag="ACZPCL_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR1161902.1"
                     /translation="MGKKSYDERNFKFETLQLHVGQEQPDPVTDARAVPIYQTSSYVF
                     RNCDHAAARFGLSDAGNIYGRLTNPTEDVFEKRIAALEGGVAALAVASGAAAIAYTFQ
                     NLAHAGDHIVAAENIYGGTYNLFAHTLPEYGIKTTFVDPFNYEQVEAAIQENTKAIHV
                     ETLGNPNSDVVDIERLAQIAHAHKIPLVVDNTFATPYLVRPIEYGADIVVHSATKFIG
                     GHGTTIGGVIVDSGKFDWEASGKFPSLVEPNPSYHGVSFVKAVGPAAFVTKIRAILLR
                     DTGATLSPFHAFFFLQGLETLSLRVERHVENALKVVQYLNNHPQVEKVHHPSVSTDPE
                     QQKLYKKYFPNGGGSIFTFEIKGDAQKAKDFIDNLELFSLLANVADVKSLVIHPASTT
                     HAQLTEEELKEQGIYPNTIRLSIGTENIDDIIEDLDEAFKAVAE"
     gene            complement(2288..3205)
                     /locus_tag="ACZPCL_05175"
     CDS             complement(2288..3205)
                     /locus_tag="ACZPCL_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1161903.1"
                     /translation="MTLMQMKYAIAVADTKSMNEAARSLFITQPSLSASIKELENEIG
                     VELFKRTNRGITVTPEGEEFLGYARQVVEQYRLIESKYIEKENVRKKFSVSMQHYSFA
                     VQAFVEMVRQFGMDKYEFAVHETKTYEVIEDVKNFKSEIGILYLNDFNSKVLTKLFTE
                     FGLEFHRLLDCGIYAYMWKGNPLASRESVTMDDLQPYPCLAFEQGNNNSFYFAEEVLS
                     TYEYKQLIRANDRATMLNLMKGLNGYTLCSGIICEELNGGDYCAVPLESDEVMTIGYL
                     CRKGVAISPLGQKYLDEIRKYKNEDARYR"
     gene            complement(3633..4979)
                     /locus_tag="ACZPCL_05180"
     CDS             complement(3633..4979)
                     /locus_tag="ACZPCL_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1161904.1"
                     /translation="MKVLEMVHGIIWGPWTVGVFLVVGLVLSVRCRFFQIRGFRYWWK
                     ATAGSLWGEEREGDESTGAAGAHHGITQFQSVCTALAATVGTGNIAGVAAALVSGGPG
                     AVFWMWISALIGMATAYGETYLGVRYRFKNNRGKWICGPFVYMKKGLGIPFMAACYSF
                     FCFLCALGMGSMVQANSAAETLEFTWGIPSVLGSGILALLTGAVILGGIKRIGKAAEY
                     LLPLASGIYILFSLLVLLLCADRIPDAFLRIFQSAFGIRQVSGGIAGFGISKSIRYGI
                     SRGVFSNEAGLGTLAVLHGPAEHTTPEEQGMWAMFEVFFDTVVLCSLTALVILCTTQG
                     DTETLSLTGAALAAACFSAKLGVIGEWFISISMVVFAFATVIAWYYLGQQAIEDLSLF
                     SRIYPVLFLGAVFAGGCIRLEAVWMLSDLWNGLMAFFNLTALLFLTGEVEYPDRYI"
     gene            5144..6130
                     /locus_tag="ACZPCL_05185"
     CDS             5144..6130
                     /locus_tag="ACZPCL_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HIR1161905.1"
                     /translation="MPGFTTHYILGMKAYNDMPQNNLKFIIAKYRWLYQLGLQGPDMF
                     FYNLPVLRHRDHRNVGSYMHEHHVNDFFRCAFTRLSKIESRQQREEGLAFLCGFISHY
                     IGDSICHPYVYGRIHYDAEHPTAACHGLHAKLENDIDALLLMKYKKKKPSQFNQAATI
                     CLNGMETQFISRFLSSCLNDAFYPLSSKNHYQVSPGMIHRSILALRLGCRTLSDPNSQ
                     KKNWIEYVESLFLRNPLASSKMVTDVVEDPVWSLNLRHETWCNPWDKSIASQTSFPDL
                     FRQCLAKHATIYYMINTLMEENNIRPASFDRILDELGNYSYHSGLPCNDEED"
     gene            complement(6152..6844)
                     /locus_tag="ACZPCL_05190"
     CDS             complement(6152..6844)
                     /locus_tag="ACZPCL_05190"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HIR1161906.1"
                     /translation="MLEELKQKVYEANMQLPKYGLVTFTWGNVSAIDREKGLFVIKPS
                     GVDYDKMKPEDMVVMDLEGNQVEGKLNPSSDTKTHLVLYNRFPMIGGIVHTHSPWATS
                     WAQAGRDIPCYGTTHADYIYGAVPCARNLTEEELKDYERNTGVLIADLFEERKLDPVA
                     VPCVLCKNHGPFTWGKDAAEAVHNAVVLEEVAKMAARCERLNPDVKEAPKCLQDKHYL
                     RKHGPGAYYGQR"
     gene            complement(6845..8452)
                     /locus_tag="ACZPCL_05195"
     CDS             complement(6845..8452)
                     /locus_tag="ACZPCL_05195"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR1161907.1"
                     /translation="MGRSVEEIRNDITNGGTSLGIEFGSTRIKAVLVDSTTAPVAQGS
                     YEWENRLENGIWTYSLEDIWKGLRSCYQDLAADVKKQYGVGLTRIGSMGISAMMHGYM
                     AFDEAGELLVPFRTWRNTITGEAAKKLTELFDYNIPQRWSIAHLYQAVLNKEEHLSKL
                     DFFTTLAGYIHWKLTGKKVLGVGDASGMFPIDPADGQYEKEMVRKFETLMEPEGYSWK
                     LSGILPQVLTAGEDAGSLTEEGAALLDESGMIQAGIPLCPPEGDAGTGMTATNSVAVR
                     TGNVSAGTSVFAMVVLEENLKKVHEEIDLVTTPDGHLVGMVHCNNCTSDLNAWVGLFE
                     QFYEMMGQKVDKNTLFGNLYRKALEGDADCGGLMSYGYLSGEPITGFEEGRPLFMRKP
                     DSKLTLANFMRTHLYSSLATLKYGMDIMFKEENVSLDKLMGHGGFFKTKGVGQRIMAA
                     AADVPVAVMETAGEGGAWGIALLAAYRKDKKEGESLSDYLNQYVFADAKEEVMEPDAE
                     DVEGFDSFMKDYIKGLAAEKGAVEGWK"
     gene            complement(8473..9978)
                     /gene="araA"
                     /locus_tag="ACZPCL_05200"
     CDS             complement(8473..9978)
                     /gene="araA"
                     /locus_tag="ACZPCL_05200"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HIR1161908.1"
                     /translation="MLMTEKKYKFWFCTGSQDLYGDECLAHVAKHSQIIVEELNKSGR
                     LPFELVWKPTLITNEVIRKTFNEANQDPECAGVITWMHTFSPAKSWILGLQEYHKPLC
                     HLHTQFNQEIPYESIDMDFMNENQSAHGGREYGHIVTRMGIERKVIVGHWADEKVQDQ
                     LASWMRTAIGVMESSHIRVIRVADNMRNVAVTDGDKVEAQIKFGWEVDAYPVNEIVEA
                     VDAVSQADTDALVEEYYDKYDILLEGRDEKEFRRHVAVQAQIELGFERFLDANNYQAI
                     VTHFGDLGGLKQLPGLAIQRLMEKGYGFGAEGDWKTAAMVRLMKIMAAGTKDAKGTSF
                     FEDYTYNLVKGREGILQAHMLEVCPTIADGKISIKECPLSMGNREDPARLVFTAKEGH
                     GIATSLIDLGDRFRLIINDVEVKKAEKPMPKLPVATAFWTPEPSLETGAAAWIYAGGA
                     HHTAFSYDLTAEQMGDWANAMGIETVYIDKDTNIRQLRNELRWNEAYYKFH"
     gene            complement(10019..11032)
                     /locus_tag="ACZPCL_05205"
     CDS             complement(10019..11032)
                     /locus_tag="ACZPCL_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1161909.1"
                     /translation="MKGKMKLKQNSFLLLVTILLFFIMYGIGCVLFNAQGFSKTQNFL
                     NLFISNAGLIVIGIGMTVVMITGGIDISVGSFVAMGCMMLAWMMEKGGIGAVPAVIIV
                     LVTGIVFGLVQGFLVAYMDIQPFIVTLAGMFFARGMTAIISKDMISITNETFMAWAKM
                     KLYLPFGGYLNKKGVMVYPYMYPTVVIALVFLVLAFIMLKYTKFGRSIYAVGGNEQSA
                     LMMGLNVRRVKLKAYVLDGFLCGVGSILFCLNTLGGFVEQAKGFEMDAIASSVIGGTL
                     LTGGVGNVFGTLFGVLIKATIEAFITFQGTLSSWWTRITIAALLCFFIVLQSVLAMMK
                     KKK"
     gene            complement(11034..12089)
                     /locus_tag="ACZPCL_05210"
     CDS             complement(11034..12089)
                     /locus_tag="ACZPCL_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161910.1"
                     /translation="MKNKTLKNITSQRLFLPIFCLALVLLVNIITTPNFFSIEIRDGV
                     LYGYMIDIINRASELVILAVGMTLVVSSSAGTDISVGAIMAVSAAVCCNILTGGERAA
                     TAYANPLILGFIAALVVGAAIGCFNGFLVSKLNIQPMVATLIMFTAGRGIAQLITDGQ
                     ITYVRVDSYRVLGASFPGFPVPTPFIVAVLVVALTSLLLKKTALGMYIQSVGINKRAS
                     QLVGIKSVLIIFLTYAYCGLCSGLAGLIASSRIYSADANNIGLNMELDAILAVAIGGN
                     SLGGGKFSLAGSVIGAFTIQALTTTLYAMHVSSDQVPVYKAIVVVIIVALQSPGFARW
                     RKSLLQKKEIKAGKEAA"
     gene            complement(12092..13606)
                     /locus_tag="ACZPCL_05215"
     CDS             complement(12092..13606)
                     /locus_tag="ACZPCL_05215"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1161911.1"
                     /translation="MEKDSVLEMRHIYKTFPGVKALQNVDFTLKKGEIHALMGENGAG
                     KSTLIKVLTGVEEFETGEIMIDGCDHTIINRSPQEAQSRGISTVYQEVNLCPNLTVAE
                     NLFIGREPRKAGLIDWKTMNKRAAKLLQDLDIHADVTQTIDNYSIAIQQMIAIARAVD
                     MSAKVLILDEPTSSLDDGEVEKLFKLMLRLKGEGVGIIFVTHFLEQVYAVCDKITVLR
                     NGQLVGEFTTAELPRVQLVAKMMGKDFDDLAAIKEAKDEEEHTEEDIVIKAVGLGKKG
                     YIKPFDLVIHKGEVVGFTGLLGSGRSETVRVLYGAERPDEGTLQVKGNGITAKHPITS
                     MHAGMAYLPEDRKNEGIIADLSVRENMIMALQAKRGMFHLLSRKQQEEFTDKYIEMLQ
                     IKTASRETPIKSLSGGNQQKVIIGRWLLTNPDFLILDEPTRGIDVGTKTEIQKLVVKL
                     ASQGMAVVFISSEIEEMLRTCDRMVVMRDGAKVGELSGNEMNQSSIMSTIAGGQ"
     gene            complement(13711..14790)
                     /locus_tag="ACZPCL_05220"
     CDS             complement(13711..14790)
                     /locus_tag="ACZPCL_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1161912.1"
                     /translation="MKKRFLSVALAAAVAMSATACTSNPAESKTTAAPAETKAEETTA
                     AEASSEGEKKEAAADGDLITVGYAQVGAESDWRTANTESFKSTFTEENGYKLIFDDAQ
                     QKQENQIKAIRSFIQQDVDYIVVAPVVETGWETVLEEAKEAGIPVILSDRQMQLSDDS
                     LYEAWVGGNFVKEGETCGDWLADYLEKQGRADEEINMVTIQGTIGASAQVGRTEGMEN
                     KLKEHSNWNMLDKQSGEFTQAKGQEVMESFLKSYDDIDVVICENDNEAFGAIDAIKAA
                     GKTCGPEGDIIVVSFDSVKAAFESMIAGDLNATFECNPLHGPRVAEIIQKLEKGEEVE
                     KIQYVDEAYFDTSMDLEEILKTRAY"
     gene            complement(14939..16438)
                     /locus_tag="ACZPCL_05225"
     CDS             complement(14939..16438)
                     /locus_tag="ACZPCL_05225"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016298268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1161913.1"
                     /translation="MEKKKKFYLSLKMRLLFLLFVIVIPLTFMVAGYQRMFENYSRSY
                     NEIMANLKVANEYNIKFKSDMEYSMYRVMIGLIDVDKFENGDILEGKSKYATVVKNPL
                     NMIASARHAFGKSIERVPGSDGDIKIKGILSCLDSLEKAVNRMIDNASVTGRYDENVN
                     IWENDIQGLCSMIQDYITQYTYYEMINMEQLQKELEQQVTKRIQEYMVLLACVLTAGT
                     FLALAITNSITGPLGRLQQTAERLGKGDLDAKADLGSLEEINVLARTFNHMSERIRRL
                     LEKNTQEQKNLREAELRLHQEQISPHFLYNTLDSIVWMAEGGNNRQVVEMTTDLSDFF
                     RTVLSGGKDFITVEEEESHIRSYLKIQKIRYEDILDYSIQIEPGIKDHIVLKMLLQPL
                     VENALYHGIKNKRGGGCIIIRGYEDRDNLIFEVEDNGSGMDPETLEKVRKKLSGTFEG
                     EQADLKKGGFGLNNVAQRLRMYYGDQAEILVESEKGIGTCFKIFLNKKT"
     gene            complement(16454..>17203)
                     /locus_tag="ACZPCL_05230"
     CDS             complement(16454..>17203)
                     /locus_tag="ACZPCL_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1161914.1"
                     /translation="QLDWSWVSTYSPKGDNFYDLNKVSNILDNTNYTGVDRKLILDFL
                     KLGAMEECDPFVEEYFAAIGENNYQSLLLRQYLTMDIFYCIQEFLKGLGEGNVTISPE
                     VTDIKRIPKVIVSVDTTKMYLKEQFQAAIEARNSVSNDRYGSVIQSAKEYIKKNFSNG
                     ELSLNRIAAHIGVSPSYFSSIFKQETGTTFVEYLTKVRIDKACELLRCTNSRTAEIGE
                     QVGYSDPHYFSATFKKVMGQSPKDYRAGQKG"
BASE COUNT         4369 a         4439 c         3360 g         5035 t
ORIGIN      
        1 gtcatcatta taattcttct cgtattcttt tccggagatc ttacatacca ggttcatagg
       61 gatgccgatg gttactgttc cttttttcag ttcccggtat tctcccagtt cttcccgcat
      121 ttccttccag agtttccata cttcctctaa ctgttctaaa agctctgcgc ccgcttttgt
      181 ctcctctgcc cccctccggt ctctctgaaa gatcttaaat cccacttcct cttccacttc
      241 ttttaagacc tggcttaagg aaggctgggc aataaaaaga gtttccgctg ccccggtaat
      301 actcttattt tcccggatac ataaaagata acggatctca ttttccgacc agatccgttt
      361 ttcctttatt tcctgcaggc tttttaagat cctctcccca taagaaagca cggtttctcc
      421 ctcggaagta gggaccattc cttcccgtat cctgcgaaac agctgacagc ccagttcatt
      481 ttccacactt ttaagtcccc tggtaagggc agaaggcgtc ctgtccacac aggccgctgc
      541 cccccggatc gtaccagtcc gtgcgatctc cctgatgaaa ataagctgct gttggttcat
      601 aaggccctcc ttctggttta tttgctctta aactctttct taaacctcat tttaccatat
      661 atccgtctga cttcccagac ctgtaaaaga atgtgcctcg gtgtatacca gggagttcca
      721 acggaatttc ctagtataca caaaacaggc gcatcttcct gttacggaag aatacgcctg
      781 tttttatata tcacttacct attttatacg atttcattca cttgccatgc ttatgtatgc
      841 tgttttactc tgctactgcc ttaaatgcct catccagatc ttcaatgata tcgtcaatgt
      901 tctcagtacc gatagaaaga cggatggtgt tcgggtagat gccctgctct ttcagctctt
      961 cttctgtcag ctgtgcatga gtagtagaag ctgggtggat aaccagagac ttaacatctg
     1021 ctacgtttgc aagtaaggag aacagttcca ggttgtcgat aaagtccttt gccttctgtg
     1081 catcgccctt gatctcaaag gtgaagatag aaccgccgcc atttgggaag tacttcttgt
     1141 acagtttctg ctgctctgga tcagttgata cagatgggtg atgaaccttt tctacctgtg
     1201 gatggttatt taagtactga actaccttca gtgcattctc tacatgacgc tctacacgaa
     1261 gggacagtgt ttccagtccc tgcaggaaga agaacgcatg gaatggggat aaagttgctc
     1321 cggtatcacg aagtaagata gcacggatct ttgtaacaaa tgctgccggt cctactgcct
     1381 ttacaaagct tacgccatgg tagcttgggt ttggctcaac taaggacggg aacttaccgc
     1441 ttgcttccca gtcaaactta ccgctgtcta cgatcacacc gccgatggtg gttccatgtc
     1501 cgccaatgaa cttggttgcg gaatgtacaa cgatgtcagc gccgtattca attgggcgaa
     1561 ccagatatgg ggttgcgaat gtattatcaa ctactaacgg gatcttatgt gcatgtgcga
     1621 tctgtgccag tctttcaata tcaactacat cagagttagg atttcccaga gtctctacgt
     1681 ggatcgcctt ggtgttttcc tggatggctg cctcaacctg ctcgtagtta aatggatcta
     1741 cgaaagtggt cttgatgccg tactctggaa gagtgtgggc aaacaggttg taagttccgc
     1801 cgtaaatatt ctcagctgct acaatgtggt cacctgcatg tgccaggttc tggaaagtat
     1861 atgcgatagc tgctgcaccg gaagctactg ctaaagctgc aacgccaccc tctaaggctg
     1921 caattctctt ttcaaataca tcttctgttg ggttggtcag acgtccgtaa atatttcctg
     1981 catcgctcag tccaaatctt gctgctgcat gatcgcagtt gcggaataca taggaagagg
     2041 tctggtagat cggtacggct cttgcatcgg ttaccgggtc tggctgctcc tgtcctacat
     2101 gaagctgtaa tgtctcaaat ttaaagtttc tttcatcgta agatttcttt cccatgttat
     2161 tgtcctcctg gattaaaaat atatcagtca tttacctact gtttttgttt gtttttgttt
     2221 atggacaaat aataacacta ctttttccat ctgtctaata cacaaaaaaa agagctcgtt
     2281 atagttttta cctatagcgg gcatcttcat tcttatattt ccgtatctca tccagatatt
     2341 tctggcccaa aggactgatg gctactccct tgcggcacag atacccaata gtcatcactt
     2401 cgtcagactc caatggcact gcacagtaat caccgccatt taattcctcg cagataatac
     2461 cggagcaaag agtatagcca tttaagcctt tcatcaggtt taacatggtt gcacggtcat
     2521 tggcacggat cagctgctta tattcataag tagaaaggac ttcttctgca aaatagaagg
     2581 aattattgtt tccctgctca aacgccagac atggataggg ctgcagatca tccatagtaa
     2641 cagactctct ggatgccagt ggatttccct tccacatata ggcatagatc ccgcaatcca
     2701 gaagcctgtg aaattccagc ccgaactctg taaacagctt tgtcagcacc ttgctgttaa
     2761 aatcattaag atataaaatg ccgatctcac ttttaaagtt cttcacatct tcgatcactt
     2821 cgtaggtctt ggtctcatga actgcaaatt catacttgtc cattccaaac tggcgcacca
     2881 tttccacaaa ggcctggaca gcaaaggaat aatgctgcat ggacacactg aacttcttgc
     2941 gcacattttc tttttcaatg tacttggact cgatcaggcg gtactgttcc accacctgtc
     3001 tggcatagcc taaaaattcc tctccttccg gcgtcacagt aataccacgg ttggtacgct
     3061 taaaaagctc cacgccaatc tcattttcca gctctttaat agacgcggaa agacttggct
     3121 gtgtgataaa caggctgcgg gccgcttcgt tcatggattt ggtatctgcc actgcaatgg
     3181 catatttcat ctgcattaag gtcatttgtg aaaaactcct ttatcattcg ggatttaaca
     3241 ttcatactgc gccacttgtt tttcttttgg agaaattata ggctaatcag gtaacgttgt
     3301 caacgctcct gaaattctca tttatgatac cggattgcta cgtgcttttg acacttgtat
     3361 cattcacatt tcgttcataa atcattcaaa aaagtgttac acaatgaaca cgatttcttc
     3421 atcttttctt tttatacttt agtcatagat gagagacaga gaaaacaaac aaaaacctca
     3481 tctcctgaaa atttttaata cctaactaag cttacaagat ttttttcata atactcgagc
     3541 tggtaagatt ttatcagctc tcctcccttt taataatact tttttccgga aaaggcacct
     3601 caaaacgagg tgctttttct agtctgctct tcctaaatat accgatccgg atattcaact
     3661 tcccctgtta aaaataaaag agccgtcaga ttaaaaaatg ccatcagtcc attccacaga
     3721 tccgacagca tccatactgc ttccagcctg atacagcctc ctgcaaatac tgctcctaaa
     3781 aataagaccg gatagatcct ggaaaaaaga gacagatctt ctattgcctg ctgtcccagg
     3841 taataccatg cgatcacagt ggcaaaagca aataccacca tggaaatact gataaaccat
     3901 tctcctatga ctcccagttt tgcagaaaaa caggcagcag ccagggccgc tcctgtaaga
     3961 gaaagtgttt ccgtatctcc ctgtgtggta cacaagatga ccagtgctgt aaggctgcac
     4021 aaaactaccg tatcaaaaaa cacctcaaac atagcccaca ttccctgttc ttctggtgtt
     4081 gtatgttctg ccggtccatg aaggactgcc aaagtgccaa gccctgcctc attggaaaaa
     4141 acgcctctgg atataccata acggatactt ttactgatgc caaagcccgc tatgccgcct
     4201 gaaacctgcc ggatcccaaa agcagactga aagatcctta aaaatgcatc tggtatacga
     4261 tctgcgcata ataaaagtac cagcagggaa aataaaatat agatcccgga agccagaggc
     4321 aacaggtatt ctgctgcttt gccgatccgt tttatcccac ctaagataac ggctcccgta
     4381 agcagcgcca gaataccgct tcctaacacc gacgggatcc cccaggtaaa ttccagtgtc
     4441 tctgctgctg aatttgcctg taccatactt cccattccaa gggcacacag aaagcagaaa
     4501 aagctgtaac atgctgccat aaaagggata cccagccctt ttttcatata gacaaagggg
     4561 ccgcagatcc attttcccct gttgttctta aaacggtacc ggactcccag ataggtttcc
     4621 ccataagcag tggccatacc gatcagtgcg gagatccaca tccagaacac ggctcccgga
     4681 ccaccggata caagagcggc tgccacacct gcaatattac cggtccccac cgttgctgca
     4741 agggcggtac agaccgactg gaactgtgta attccatggt gggccccggc agcacctgta
     4801 ctttcatcgc cctcacgttc ctctccccat aagcttccgg cagttgcctt ccaccaatat
     4861 cgaaatcccc ggatctgaaa gaaacggcag cgcaccgata acaccagtcc cacaaccaga
     4921 aacaccccca ccgtccacgg cccccatatg atcccatgca ccatttccag aactttcata
     4981 tgcgccttat ttatgcctca ctgcaacctt cttatgaggt ttttatgcac tgtttttccc
     5041 ttctatgcct gctttatcca caaaaatatg gtataatgta aagagcgcag gaacctgcgt
     5101 ggccccacaa cgccatcaac acccgaaagg aggccccaca accatgcccg gcttcaccac
     5161 ccactacatt ctcggtatga aggcatacaa tgacatgcct cagaacaact taaaattcat
     5221 cattgcaaaa taccgctggc tctaccagct tggtcttcag ggaccggata tgttttttta
     5281 taatctgcct gttttgcgcc acagggatca ccgcaacgta ggctcctata tgcatgaaca
     5341 ccatgtaaat gactttttcc gctgcgcatt cacccgtctt tcaaaaattg agtcccggca
     5401 gcagcgtgag gaaggccttg ctttcttatg cggtttcatc agccattata ttggcgactc
     5461 tatctgccac ccttatgtat atggccgcat ccactatgac gcggagcatc caacagccgc
     5521 ctgtcacggt ttacatgcca agctggaaaa tgacattgat gccctgttat tgatgaaata
     5581 caaaaagaaa aagccgtccc agttcaacca ggcggctacc atctgtttaa atggcatgga
     5641 aacacagttt atctcccgtt tcctctcctc ctgcttaaat gacgccttct atcctttaag
     5701 cagcaaaaat cattatcagg tatccccggg aatgatccac cgttctatcc ttgccctgcg
     5761 tctgggctgc cggacacttt ccgacccaaa cagccagaaa aagaactgga tcgaatacgt
     5821 agagtccctg tttttaagaa atcctttagc ttccagtaaa atggtaaccg atgtggtaga
     5881 agaccctgta tggagcctga acctgcgcca tgagacctgg tgcaatccct gggacaagag
     5941 cattgcttcc cagacctctt tcccggatct gttccgccag tgccttgcaa aacatgccac
     6001 catctactat atgatcaaca ctttgatgga agaaaataat attcgcccag catcctttga
     6061 ccggatactg gacgaacttg gaaattattc ttatcacagc ggtcttccct gcaacgacga
     6121 ggaagactga cgcatcataa ttcttatagc attaccgctg tccataataa gcgcccgggc
     6181 catgcttgcg cagataatgt ttatcctgca ggcacttagg cgcttctttt acgtctggat
     6241 ttaaacgctc acagcgggct gccattttag caacttcttc cagtactact gcattgtgta
     6301 ctgcctctgc cgcatctttt ccccaggtaa aaggaccatg atttttacac agaacacagg
     6361 gaactgccac cggatccagt tttctttctt caaacagatc cgcgatcaga actcctgtat
     6421 ttctttcata atcctttaat tcttcctctg tcaggtttct ggcacaggga actgccccgt
     6481 aaatataatc tgcatgggtg gttccgtaac acgggatgtc tcttccggcc tgtgcccagg
     6541 aagtagccca tggactgtgg gtatgtacga taccaccgat catcggaaaa cggttgtaca
     6601 gtaccagatg ggtctttgta tcagaagacg gatttaattt tccctctacc tgatttccct
     6661 ccaggtccat aaccaccata tcttccggtt tcattttatc gtaatctacc cctgaaggct
     6721 tgatcacaaa cagtcccttt tccctgtcaa tagcagaaac atttccccag gtaaaagtga
     6781 ccagaccata ctttggaagc tgcatattgg cttcatatac tttctgtttt aattcttcca
     6841 gcatttattt ccatccttcc actgcgccct tttcagctgc cagtcctttg atataatcct
     6901 tcataaagct gtcaaagccc tctacatcct ctgcatccgg ctccattact tcttctttgg
     6961 catccgcaaa tacatactgg ttcagataat cagaaaggct ttccccttct ttcttatctt
     7021 ttctgtaagc tgctaaaaga gcaatgcccc aggcgccgcc ctctcctgct gtctccataa
     7081 cagcaacagg tacatctgct gcagcagcca taatgcgctg tccaacacct tttgtcttaa
     7141 agaaaccgcc gtgtcccatt aatttgtcca gagacacatt ctcttcttta aacataatgt
     7201 ccatgccata tttaagggtt gcaagggaac tgtataaatg agtcctcata aagttagcca
     7261 gtgtcagttt gctgtccggt tttctcataa acagaggacg tccttcttca aaacctgtga
     7321 tcggctctcc ggaaagataa ccataagaca taaggccgcc gcagtcagca tccccttcca
     7381 gggcttttct gtaaagatta ccaaataaag tatttttatc cactttctgg cccatcatct
     7441 cataaaactg ctcaaacagt cctacccagg cattcagatc cgaagtacag ttgttgcagt
     7501 ggaccattcc taccagatga ccgtcaggtg tagttacaag gtcgatctcc tcatggacct
     7561 tctttaagtt ctcttccagg actaccatgg caaatacgga agtaccggca gatacatttc
     7621 ctgtacggac agctacactg tttgtagcag tcataccagt tcctgcgtct ccctctggcg
     7681 gacaaagtgg gatacctgcc tgaatcattc cgctttcatc taaaagggct gctccttctt
     7741 ctgtaagact tcccgcatct tctcctgcag taaggacctg aggcaggata ccggataatt
     7801 tccaggaata tccttccggc tccatcagtg tttcaaactt gcggaccatt tctttctcat
     7861 actggccatc tgccggatcg attgggaaca ttcctgaggc atctcctacg cctaaaacct
     7921 tctttcctgt cagcttccag tggatatatc cagccagagt ggtgaagaaa tccagctttg
     7981 acagatgttc ttctttgttt aacactgcct ggtataaatg ggcaatgctc catctttgcg
     8041 gaatattata atcaaacagt tcggtcagtt tctttgcagc ttcccctgta atggtatttc
     8101 tccaggttct aaatggcacc agcagttctc ctgcttcatc aaatgccata tatccgtgca
     8161 tcatggcact gatacccata gagccgatcc gtgtaagtcc cactccatac tgctttttta
     8221 catctgctgc cagatcctga tagcaggaac gcaggccttt ccagatatcc tccagggaat
     8281 aggtccagat cccgttttcc aggcggtttt cccactcgta acttccctgt gcaacagggg
     8341 cggtggtact gtcaaccagt accgccttga tcctggtaga accaaactcg atccccagag
     8401 atgttccccc gtttgttatg tcatttctta tttcttctac actgcgaccc atagctcctt
     8461 ctttcttcct gatcagtgga acttgtaata agcctcattc cagcgcagtt cattgcgcag
     8521 ctgacggata tttgtatctt tgtcaatata aacggtttca attcccattg cattggccca
     8581 gtctcccatc tgctctgcag tcaggtcata ggaaaaagca gtatggtgcg cgccgcctgc
     8641 atagatccat gctgcagccc ctgtttcaag agatggctcc ggagtccaga atgcagttgc
     8701 taccggaagt ttcggcattg gtttttctgc cttctttact tccacatcat tgatgatcag
     8761 acggaagcgg tctcccaggt caataaggga agttgcaata ccatgtcctt cctttgcagt
     8821 aaataccaga cgggccggat cttccctgtt gcccatggaa agcgggcact ctttaatgga
     8881 gatctttcca tctgcgatgg tcgggcaaac ctctaacata tgagcctgaa ggataccttc
     8941 tcttcctttt accagattgt atgtataatc ttcaaagaag ctggttccct ttgcatcctt
     9001 tgttcctgct gccatgatct tcatcagacg taccattgcc gctgtcttcc agtcaccttc
     9061 tgcgccaaat ccatagcctt tttccatcag tcgctggatc gcaagtcccg gaagctgctt
     9121 taagccgccc agatcaccaa aatgggttac gatcgcctga taattgttgg catctaagaa
     9181 ccgttcaaag cccagctcga tctgtgcctg tacagctaca tgacggcgga attccttttc
     9241 atccctgcct tctaagagga tgtcatattt atcataatat tcttctacca gggcatctgt
     9301 atctgcctgt gaaacagcgt ctaccgcttc tacgatctca tttacaggat aagcatctac
     9361 ttcccagcca aatttgatct gtgcctctac cttatcaccg tctgtaactg ctacgttgcg
     9421 catattatcg gcaacacgaa ttacacggat atgggaagac tccattactc cgatggctgt
     9481 acgcatccag gaagccagct gatcctgtac tttttcatct gcccagtggc ctacgatcac
     9541 ttttctttca atccccatac gggtcacaat atgtccatat tctcttccgc cgtgggcaga
     9601 ctggttttca ttcatgaaat ccatatcaat ggactcataa gggatctcct ggttaaactg
     9661 ggtatgcaga tggcataaag gcttgtgata ttcctgtaat cccaggatcc agcttttagc
     9721 cggtgaaaaa gtatgcatcc aggtgataac gcctgcgcac tccggatcct gatttgcttc
     9781 gttaaatgtc ttccggatca cttcattggt gatcagagtc ggcttccata caagctcaaa
     9841 tggaagtctt ccgctcttat tcaactcttc tactatgatc tgtgaatgtt tcgctacatg
     9901 agccagacac tcatctccgt aaagatcctg tgaaccggtg caaaaccaaa atttatattt
     9961 tttctccgtc atcagcatag caaacttctc cttatttttc tacatttttc tattttttct
    10021 acttcttttt cttcatcatg gcaagaacag actggagtac aatgaagaag cataacagag
    10081 ctgcaatggt aatacgtgtc caccaggaag ataaggttcc ctggaaggtg ataaaggctt
    10141 cgatggttgc cttgatcagt acaccaaata aggttccaaa tacatttccc acaccaccgg
    10201 taagaagggt tccaccgata acggaagaag caatggcatc catttcaaag ccctttgcct
    10261 gctctacgaa accgcccagt gtgtttaagc agaataaaat acttccaacg ccgcagagga
    10321 aaccgtctaa tacataggct tttaatttga ccctgcgtac atttaagccc atcatcaggg
    10381 ctgactgctc atttccgcct actgcataaa tgctgcgtcc aaacttggta tattttaaca
    10441 tgataaatgc cagtaccaga aataccaggg cgatcacaac ggtaggatac atataaggat
    10501 agaccataac gccctttttg ttcaggtagc cgccaaatgg cagatacagc ttcatttttg
    10561 cccatgccat aaaggtctca ttggtaatac tgatcatatc tttggaaatg atggcagtca
    10621 tacctctggc aaagaacata cctgccagtg ttacgataaa tggctggatg tccatgtagg
    10681 caactaaaaa tccctggacc aggccaaata caatgcctgt caccagaaca atgatcactg
    10741 caggaactgc accaataccg cctttttcca tcatccaggc aagcatcata cagcccatag
    10801 ccacgaaaga accaacgctg atatcaatac cgccggtgat cataacaaca gtcatgccga
    10861 taccgataac gatcagtcct gcattggata taaacaggtt taagaagttc tgtgtcttgg
    10921 aaaagccctg tgcgttaaac agcacacagc cgatcccgta cataataaag aacagcaaaa
    10981 tggtaacaag cagcaggaaa ctgttctgtt ttaatttcat tttacctttc atgtcatgct
    11041 gcctcctttc ctgcttttat ctctttcttc tgtaacagag attttctcca tcttgcaaaa
    11101 cctggagact ggagagctac aatgataaca actacgatag ccttataaac aggcacctgg
    11161 tcagaagaaa catgcattgc atacagggta gttgtaagtg cctggatggt aaatgcaccg
    11221 atcacggaac ctgccagtga aaattttccg ccgcctaagg agtttccgcc aatggcaact
    11281 gccagaatgg catccagttc catgttaaga ccaatgttgt tggcatctgc agaataaata
    11341 cggctggaag cgatcaggcc tgcaaggccg gaacacaggc cgcagtacgc atatgtaagg
    11401 aaaatgataa gaacggattt aatacctact aactgggatg ctctcttgtt gatacctaca
    11461 gactggatgt acatgcccag ggctgttttc tttaaaagca gcgaagtaag ggcaactacc
    11521 agcactgcca cgataaacgg ggtaggaacc ggaaatcccg ggaaagaagc gcccagtaca
    11581 cggtaactgt ctacacgcac ataggtgatc tgtccgtctg tgatcagctg tgcaatacct
    11641 ctgcctgctg taaacatgat cagagttgcc accataggct ggatatttaa ctttgatact
    11701 aaaaatccgt taaaacagcc aatggctgcg cctactacca aagctgctat aaagccaagg
    11761 atcagtggat ttgcataagc agtagctgct ctctctccac ctgtaaggat gttacagcat
    11821 acagctgcac ttactgccat aatagcgcct acggaaatat ccgttcctgc tgaggaagat
    11881 actaccaggg tcattcctac tgccaggatc acaagttcgg aggcacggtt gatgatatcg
    11941 atcatatatc catagagaac gccatcacgg atctcaatgg aaaagaagtt cggtgttgtg
    12001 atgatattta ccagcaatac cagtgccagg cagaaaatag ggagaaataa tctctgggat
    12061 gttatgtttt ttaatgtttt atttttcatg actactgccc tcctgcaatg gttgacataa
    12121 tggaagactg gttcatttca tttccggaaa gttctcctac ctttgcaccg tcacgcataa
    12181 ccaccatgcg gtcacaggta cggagcatct cttcgatctc tgaggaaata aataccactg
    12241 ccattccctg ggaagccagt tttactacca gcttctggat ctctgttttg gttcctacgt
    12301 caatacctct tgtaggctcg tccagaataa gaaaatccgg attggtcaaa agccagcggc
    12361 caatgatcac cttctgctga tttccaccgg ataatgattt gatcggtgtt tcacggctgg
    12421 ctgttttgat ctgcagcatt tcaatatatt tatctgtaaa ttcttcctgc tgctttctgc
    12481 ttaataagtg gaacatgcca cgttttgcct gaagtgccat gatcatattt tctcttacag
    12541 acaggtctgc aatgatacct tcatttttac ggtcttccgg aagataggcc attcctgcat
    12601 gcatggaagt aattgggtgt ttggctgtta ttccatttcc ctttacctga agggttcctt
    12661 catccggacg ttctgccccg tagagaacac ggactgtttc agatcgtccg gaacctaaca
    12721 ggcctgtaaa gccaacaacc tcgcctttgt ggatcaccag gtcaaatggc ttgatatagc
    12781 ctttctttcc cagtcccact gccttgataa cgatatcttc ctctgtatgt tcctcttcgt
    12841 cctttgcttc cttgatggca gccagatcat caaaatcttt acccatcatc tttgcaacca
    12901 gctggactct tggaagttct gctgtggtaa attctcctac cagctgtccg ttacgcagta
    12961 ctgtgatctt atcgcagact gcatatacct gctccaggaa atgggtaaca aagataatgc
    13021 ccactccctc tccttttaaa cgaagcatca gcttaaacag cttttccact tctccgtcat
    13081 ccagggaaga agtaggctca tccaggatca gaacctttgc agacatgtcc actgcacggg
    13141 caatggcgat catctgctgg atggcgatgg aataattgtc aatagtctgt gtaacatctg
    13201 catgtatgtc aagatcctgc aataattttg cagctctttt attcatagtt ttccagtcga
    13261 tcagccctgc ttttctgggc tctcttccaa taaaaagatt ttctgccact gtaaggttgg
    13321 ggcagaggtt tacttcctga tacacggtac tgataccgcg gctctgggct tcctgcggac
    13381 tgcggttgat gatcgtatgg tcacagccat cgatcatgat ctctcctgtt tcaaactcct
    13441 ccacaccggt gagaaccttg atcagagtgg attttccggc tccgttctca cccataagtg
    13501 catgtatttc cccttttttt aatgtaaaat ccacattctg cagagctttt acacctggga
    13561 atgtcttata tatatgccgc atttcaagga cagaatcctt ttccataaaa gccatgcctc
    13621 cttttttcta aaaaaagaca cgggagggga cttttgtctc ccgccccgtg cctgatctgc
    13681 tatgtattac tggtaacaga ttgctggtta ttagtatgct ctggtcttta agatctcttc
    13741 cagatccatg gaggtatcaa agtaagcttc atctacatac tggatctttt caacttcttc
    13801 gcccttttcc agtttctgga tgatctcagc tacacgtggt ccatgaagtg ggttgcactc
    13861 gaaggttgcg tttaagtcac ctgcgatcat agactcaaat gctgccttta cagagtcaaa
    13921 acttactacg ataatgtcac cctctggtcc acaggtcttt cctgctgcct tgatcgcgtc
    13981 gatagcaccg aatgcttcgt tatcattctc acagataact acgtcaatat catcgtagct
    14041 ctttaagaag gactccataa cttcctgtcc tttagcctgt gtaaattcac cggactgctt
    14101 gtctaacata ttccagttgg aatgttcttt taacttgttc tccatacctt ctgtacggcc
    14161 aacctgtgca gatgcaccga tggttccctg aatggttacc atattgatct cttcgtctgc
    14221 tctgccctgt ttctctaagt aatctgccag ccagtcacca caggtctcgc cttcttttac
    14281 gaagtttccg cctacccatg cttcataaag gctgtcatct gaaagctgca tctgacggtc
    14341 agaaagaata accggtattc cagcttcttt tgcttcttcc agaacagttt cccatccggt
    14401 ctcaactaca ggagcaacta cgatataatc aacatcctgc tggataaagg aacggatcgc
    14461 tttgatctgg ttttcctgtt tctgctgtgc gtcatcaaag atcagcttat agccgttttc
    14521 ttctgtaaat gtggacttaa aagattctgt attcgcagtt ctccagtcag actcagcgcc
    14581 tacctgtgcg tatccgactg tgatcagatc tccgtcagct gctgcttcct tcttttcccc
    14641 ttcagaagaa gcttctgctg ctgtggtctc ctctgccttt gtctcagctg gcgctgcagt
    14701 tgtttttgac tctgcaggat tgctggtgca tgcggttgct gacattgcta cagctgctgc
    14761 taatgctacg gataaaaatc tttttttcat atgatggttc ctccttttta ataacatcac
    14821 atttgttttt gcagttttat tatacaaaat gctccgtata ttttatacag agcatctttt
    14881 caaaaaagtg tgttttttat aggttttcca caaatttttg tgttttcttt ggaaaaagtt
    14941 atgttttttt attgaggaag atcttaaagc aggtcccgat ccctttttca gactcaacca
    15001 gtatctctgc ctgatcccca taatacatcc taaggcgctg tgccacatta ttaagaccaa
    15061 agccgccttt tttcagatct gcctgttccc cttcaaaggt tccggaaagc tttttacgta
    15121 ctttttcaag agtttccgga tccatgccgc ttccattatc ctccacttca aagatcaggt
    15181 tatcccggtc ctcatagcct cttatgataa tacagcctcc gccccgctta tttttgatcc
    15241 cgtggtacag agcgttttca actaaaggct gcagcagcat ttttaaaaca atatgatcct
    15301 ttattcccgg ttcgatctga atggaatagt ctaaaatatc ctcatagcgg atcttctgga
    15361 tctttaaata actgcgtata tgagactctt cttcctccac tgtaataaaa tcttttcctc
    15421 ccgacagcac ggtgcggaag aaatcactga ggtctgtggt catttccact acctgacggt
    15481 tatttccccc ttctgccatc cacacaatgg agtctaaagt attataaaga aaatggggac
    15541 tgatctgctc ctggtggagc cgcagctctg cctcccgcag atttttctgc tcctgggtat
    15601 ttttctctaa cagccgccgg atccgctcac tcatatgatt aaaagtacgg gccagtacat
    15661 tgatctcctc taaactgcca agatccgcct ttgcatccag atctcccttt cccagacgtt
    15721 ctgccgtctg ctgcagccgt cccaaaggcc ctgtaatgga atttgtgatg gccagtgcaa
    15781 ggaaagtgcc tgctgtcagc acacaggcca gcaaaaccat atattcctgg atccgtttgg
    15841 tcacctgctg ttccagttct ttttgcagct gctccatatt gatcatctca tagtaggtat
    15901 actgggtaat ataatcctgg atcatggaac acagcccctg gatatcattt tcccagatat
    15961 ttacattttc atcgtatctt cctgtaacag aggcattgtc gatcatccgg ttgactgcct
    16021 tttccagaga gtctaaacag gataaaatac cttttatctt aatatccccg tctgatcccg
    16081 gtacccgctc aatggacttt ccaaaggcat gacgggcaga tgcaatcatg ttcaggggat
    16141 ttttcaccac tgtagcatac ttgctcttcc cctctaaaat atccccgttt tcaaatttat
    16201 ctacatcgat cagaccgatc atgacccggt acatggaata ttccatatcg gatttaaact
    16261 tgatattgta ttcattggcc actttcaggt ttgccatgat ctcattatag gaccgggaat
    16321 aattttcaaa catacgctga tagccggcta ccataaatgt aagagggatc acgatcacaa
    16381 acagtaaaaa gagcagacgc attttcagtg acaggtaaaa cttctttttc ttttccatgt
    16441 ctctatgccc cttttatcct ttttgtcctg ccctgtagtc ttttggtgac tgccccatca
    16501 ctttcttgaa agtagcgctg aaataatggg gatcactgta gcctacctgc tccccgatct
    16561 ctgctgtgcg gctgttggtg cagcgcagaa gttcacaggc tttgtcaatg cgtactttcg
    16621 taagatattc cacaaaagtc gtcccagttt cctgcttgaa aatagagctg aaatagctgg
    16681 gactgactcc tatatgggcc gcgatcctgt ttaatgacag ttctccgttg gagaagttct
    16741 tttttatgta ctctttggca ctttggatca cacttccata acggtcattt gagacactgt
    16801 tccttgcctc gatggctgcc tgaaactgct ctttcagata catcttggtc gtatccacgc
    16861 tgacgatcac ttttgggatc cgcttaatat ccgtaacttc cggtgaaatt gttacatttc
    16921 cctctcccag tccttttaag aattcctgga tacagtagaa aatatccatg gtaagatact
    16981 ggcgcagcag caatgactga taattgttct caccgatcgc agcaaaatat tcttccacaa
    17041 aaggatcaca ttcttccatt gctcctaact tcaaaaaatc caggatcagc ttccggtcta
    17101 cccctgtata attggtattg tccaggatat tggatacctt gttcagatca tagaagttgt
    17161 ccccttttgg tgagtaggtg gacacccagc tccagtctaa ctg
//
LOCUS       DBMDUA010000044        17085 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1944_length_17085_cov_4.477979, whole genome shotgun sequence.
ACCESSION   DBMDUA010000044 DBMDUA010000000
VERSION     DBMDUA010000044.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 17085)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17085)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17085
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1944_length_17085_cov_4.477979"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(157..1380)
                     /locus_tag="ACZPCL_05235"
     CDS             complement(157..1380)
                     /locus_tag="ACZPCL_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161915.1"
                     /translation="MVEVSAAKRNITPPFPMYMRGYAMRTGKSIGVLDELYCRTLVLR
                     IDGEIFIWSTLDLCRLEEPISDYARTVLAGKYSVPKENIIIGTIHTHSGPDISFEDEG
                     EDRNHRKAVYRELVMKQLFDAVDECFDRGFLEVTPYMVKGTIEGVYGNRNYIDKPSDK
                     DINMILFRNENHVVAGMFQFTCHPTVLGIHNMKISSDLLGNVGKALDEKYNTIFITMQ
                     GACGDMGNRQYRQGNDENELWRVRDEVMKQINVFAEAETPMELKAGSVKTAEYTIHQT
                     YDLDTMKAQLAEDEKKLAAAVTEDDKKLLWSGVRHMRRKIQSGGINVTLRSVIFHLGD
                     LEMITIPGELFSTFGMEIKKNFNAPMRIVWGYANYSAGYIVEKDEFGKGYESMQTPFL
                     QGEAELYVDHLIKSL"
     gene            complement(1505..2326)
                     /locus_tag="ACZPCL_05240"
     CDS             complement(1505..2326)
                     /locus_tag="ACZPCL_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIR1161916.1"
                     /translation="MNNENEVMEKAPVLTKKDCVKGAIRWSLMAITTFNYDTQLAPAV
                     VFGIGPLLRKIYKDDDEYVEALNNHYKYFNTMPWLANIVLGATLALEDKEGITSLDAV
                     QNIKVSLMGPLAGIGDTLFWTLLPTIMGSIAGYMALEGNPIGVILWLIVNLIFICIRT
                     QLMWIGYREGTKLITKVGSKLARITDAASVLGLTVVGALSATVVTAKTPLAFQMGEVN
                     LAVADLLDKIMPSMISVATVLVLYKLLGKKGMKITTLILIVIIFSMICSALGILA"
     gene            complement(2319..3089)
                     /locus_tag="ACZPCL_05245"
     CDS             complement(2319..3089)
                     /locus_tag="ACZPCL_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIR1161917.1"
                     /translation="MQLSALQIVLLVLVAAEINVDRRGLSLTHARPVIVGFLCGLILG
                     DMNSGLYIGGTFTLMSLGVAALGGSSVPDYGVATIIACAFAKTTGQGPEVGMTIGLPV
                     GMLGVQMDVLYKICNSFIAQKSQKYANEKQFGKMYAILYLCPVMVAVFMAFPTAVALT
                     AGAPLVQAILDVLPSWVTGGLSVAGKLLPGLGIAMLLHYMPAKKYFNYILIGFVLSAY
                     MGVPILGIAFLGVALAYKFYMDEEAKENVGFAGGLEDE"
     gene            complement(3108..3593)
                     /locus_tag="ACZPCL_05250"
     CDS             complement(3108..3593)
                     /locus_tag="ACZPCL_05250"
                     /inference="COORDINATES: protein motif:HMM:NF015769.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HIR1161918.1"
                     /translation="MGIVLARIDNRLLHGIVATQWAGRSGAQRIMIIDDTVANNELTK
                     ASMKLARPTGMAISIITEETALNNFKAGKYNDHTVFVLVKKPETLVKLSEIGVKIPEL
                     VIGGTVKPAEGEEAVKLSQRAYAKPDDIEAYKKLKAAGTKLYAQYVPADAEVSIDEFL
                     K"
     gene            complement(3603..6371)
                     /locus_tag="ACZPCL_05255"
     CDS             complement(3603..6371)
                     /locus_tag="ACZPCL_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012385.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIR1161919.1"
                     /translation="MLSTKEELYLYLEKISRGYALKDLKYFTANHISESLNISRNLAS
                     QYLNELVKEGRAIKVNSRPVYFFHKKNVEREAQVALETCVFSGMDDFQRKCRSDENKR
                     DFQKAIGYYLSLSSCIEQCKAAVKYPPNGLPALFNGAGGTGKAFLSRLMYEYAVNERV
                     IGTAGAASLPPYITVDCSEYAQNEEKFAISLCGSEDGKGWLAKANGGILFFDEIDRLP
                     FSGQELIYSYLISGQYKGYHDDEHVFESNARLVFSTTKVPAETLYKPLARMIPIVIPV
                     PTLHERTADEKEEMLVSFLKAEGRRMGVDVSISQNAFHCMLEFSYENNIDELKTCVTS
                     SCAGAYLEKTSDGIAIHSYHLPEYMLSSLKLEVEDKDKRMIHLNSYSRDTSLGQTVQY
                     FQIMLDVFEDYRQDMLSFDGLMMEFRKQLNDYYDYLIYGQKLVNIKISTYEQLINQIF
                     ENVGEMYGISLSKKYSHLLARRLYIQLQGDHLIDNWMKQNHDEIEEMFQAVSAVLEKE
                     SVICSRIVNLIKLNLDIEADSLNQLFLLLDIKNQNQKIQGLSAAGIILSHGYATASSI
                     ADAANQIIGRKVFDAIDMPLDMQMSDITGLLEKHIERFITCQDVVLLVDMGSLEQIHS
                     QMGGLRNLNIGIINNISTALAVDIGLGICGNQKLEDILKRASESNVCTYRIIHNVQKE
                     DAVIFSGENGIDTTEKLKELILKTSATSIPVKFVAYDYYRLMKNGSHDEIFQQYHVKC
                     IIGLFNPEIEGIPFISLEDIISMNSAEKLTNIFSEYLDEEQMEIFNQNLVKNFSLQNV
                     VESITILNPDKLLDEVEQAVGRLQKITGRKIAGRIMIGLYVHLCCLVERLVTKTPIDN
                     YQDLEEFEQKHADFIRQVRDSFQDISRHYRVALPVSEIAYIYDYMHLNSKNKLSGQAE
                     SPAVREDE"
     gene            6682..7506
                     /locus_tag="ACZPCL_05260"
     CDS             6682..7506
                     /locus_tag="ACZPCL_05260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1161920.1"
                     /translation="MHSEKTTIRLVAVDMDGTLLHDDKSISDYTLDVLRKIVKKGVIL
                     VPASGRPIGGMKEAVLNNVDGVQYAICSNGAVLMDVPEEKSICETGISTEKAVEAIAY
                     LEQFPVAVYVHTDRGTFRAEGWEKTGLPEKYPYIRFSEGNVKDLGEFLRTSGVKVMKM
                     GAFVLKDGLAQELLEKGSPIPGIVFLRTGDGIIELNSVDASKGNALCTLCKKLGISME
                     NVLAIGDNENDISMLQAAGISVAMENAEDDVKQAAKFVAGHNEEDGAAHFLEEWVL"
     gene            complement(7599..8987)
                     /locus_tag="ACZPCL_05265"
     CDS             complement(7599..8987)
                     /locus_tag="ACZPCL_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1161921.1"
                     /translation="MNKKKSNFSGQLGFVLAAAGSAVGVGNLWRFPYLAAKDGGGLFL
                     VIYLALVLTFGFTLLASDIAIGRRTKESAIGAYTQMSPKWKFLGILTFLVPVLIMTYY
                     AVIGGWVTKYAVVYLTGQTAAAAQDNYFTSFITSPVSPVLFALLFMGVTAFIVYNGVE
                     GGIERVSRCMMPILLVLVVVIAGYSLTLHHVDESGQMRTGMDGLLYYITPHFEGLTFS
                     RFLQILLDAMSQLFFSLSVSMGIMITYGSYVKPEVNLNKAVNQIEIFDTGVALLAGAM
                     IIPAVFVFSGTDGMSAGPSLMFISLPKVFAAMGKAGTFVGLLFFVTTIFATLTSCISV
                     LESITANCMEIFHTGRKKTVLVLTVIYLAASAVIALGYSIFYIEVKLPNGSVGQLLDI
                     MDYISNSVMMPFIALLSAFLIGWIKTPDYVIEEMESTGDTFRRKKLYRVMIRYVVPVM
                     MFVLFLQSTGIL"
     gene            9128..10000
                     /locus_tag="ACZPCL_05270"
     CDS             9128..10000
                     /locus_tag="ACZPCL_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1161922.1"
                     /translation="MKEQKDRYEIFLKVCETGSFSGAAEALNYTQSGISQMMAGLEEE
                     LEVRLFARVKKGVILTDNGARLIPYIQEMVNQKAKLRQAAFNINHKIEGKLRVGSFSS
                     ITALWMPGVIHFFKEHYPKVQVEIFDGNYDEIRDWIIHGQVDCGFLSSIVADDLKFYP
                     LMDDPLCAVMPKGHPLTEKERVTVSELFSYPLIIETPGCDNDIQHLLAKCSVKPKITY
                     SFRDDTLIMAFVKSGLGVTISQKMVLKAFARDDVESRPLEPASHRTLGLAFSKTANSV
                     VSGIMLEYLKKMTG"
     gene            10173..12095
                     /locus_tag="ACZPCL_05275"
     CDS             10173..12095
                     /locus_tag="ACZPCL_05275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR1161923.1"
                     /translation="MRTLKKSIWSYMYKVTFALVSVILISITALNIANEQSRAQGTAD
                     KIFEQMDKMLEENQKELTRLRQEYAAKCLHNTEAIAYILEKNTGARNDLYELRKIAEF
                     MEVDEIHIIDAAGEIVSGTHPQYYGYSFDSGEQMNYFKPMLKDKSLKMVQDIEPNTAE
                     HKLMQYSAMWNSTGEFIVEAGIEPVNVSKVTEKNELPYIFSQLCVNPDTSYFAVNAET
                     EKIVGATDTELVGMDMKEIGLNTEKIHSTKGFSGMINGKRSYIVFKKVADNYIGVAVT
                     SKALYERIPLNMLYLAVCLVLIALALFKAVTRYLDREVVQKIGEVNGKLHKITMGDLN
                     VKIDVHSSREFFELSRYINEMLQSLLVNDRKMTYVLGKTDMHIGVYEYNRQMKRVRFT
                     GDILQILKTGTEAAWKLPSDWEDFKKYIRTMQKEPVAGENEIFAVGNGKYLKIEEIQE
                     GEEIFGVITDVTEEIRKRREIEYQRDHDQLTGLFNRTGRDTRLSKILEECREPYYCAA
                     VMVDADGLKMVNDTYGHENGDEYLKRIAEMLKQEAGENSILCRQGGDEFTLFYYKYGK
                     EALVQKIEALKALQSGQKVRLRDGLTVDLRFSMGSSMAYGAVDYDKMYREADEKMYED
                     KRMRKKLAEETNTIES"
     gene            12337..13851
                     /locus_tag="ACZPCL_05280"
     CDS             12337..13851
                     /locus_tag="ACZPCL_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008753954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1161924.1"
                     /translation="MAELLRMEGITKQFGAVYANKDINLSIEEGEIHTLLGENGAGKS
                     TLMNILIGLYQPTSGTIWLKGKKVKIDSPKTAVKLGIGMVHQHFMLVDAMTVFENIIL
                     GTKKDSSIFIKKEERKKEILELSQKYGLEVDLDKQVNEISVGEQQRVEILKALYRGAE
                     LLILDEPTAALTDQEVEGLFSIMGRLTAENKSVIFISHKMREVMAVSDRVTILRAGQT
                     ITTVDKKDTDGAQLANLMIGHEMTVSAYKKVTEPGEDVLRLLHVDYQKDHKHSGLNNV
                     SFSIGKGEILGVAGVDGNGQSQLAQLVTGVISPDSGEVELNSHKVAQFAPNGFILSNV
                     SHVPEDRNKMGLVGNMTVEENLVLKATEEPRFSYAHGALLKKKAIRKFALELQKKNDI
                     RCASIEQEARNLSGGNQQKIILAREMENHPELLVAVHPTRGLDIGASQYVHDTMIEAR
                     DKGCGILLISADFDEVLKLSDRILVMFEGQVMGIYSGENPPVEEISLAMAGKEE"
     gene            13856..14917
                     /locus_tag="ACZPCL_05285"
     CDS             13856..14917
                     /locus_tag="ACZPCL_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009446224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161925.1"
                     /translation="MKLKDNLAKILIPILSILIAFIAGGIIILCLGKNPVEAYGYLFS
                     GAFGSGRKMGQTLVIACPLIFTGLAAAFAYKCGVFNLGGEGQFIMGAVTSIFVSAKLG
                     DAGMGGLLVSLLAGTVAGGIWGAIPGILKITRGLNEMIVSIMLNYVATLFMGYVYTTL
                     LRDGSVPQTVAVADSMKIAGISKGFPAHWGVLAAFLLAAVLYYFIFYTSKGFKLRAVG
                     MNATAAFFNGFPVNKYILFSFITSGAIAGLGGSIELHGKQFRLMSGFGSGFGFDGVAI
                     ALIAQLNPLGTAIVACIFAVLRKGASTLQTATQVPSSVVDIIQALVIIFAVAGTALLK
                     LPTIKQFLINHGKRKEAQG"
     gene            14917..15858
                     /locus_tag="ACZPCL_05290"
     CDS             14917..15858
                     /locus_tag="ACZPCL_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008753953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161926.1"
                     /translation="MASFFTMNFLTELLLSTVRMATPILLVALAETYSERAGMVNIGL
                     DGIMAIGALVGFLVGYETGSPWAGVLAGALSGIATNMIYAFCTITLCAEQTVYGMAIN
                     IFAPALASFIYRLSFSDTSTLIQGVSMGIMPIPVLSKIPMIGSIFFQQSPLVYGAYLL
                     VAVTAVFLNRTKAGLNFKAVGEFPKAAETLGINVVLQKYIACIICGALAGIGGAYLTI
                     CYTSTYSEGIVAGRGFIALSAVIFGRWMPTGVMFACLLFGFCDALQIRLQLLSPSTPY
                     QILQMIPYLCTLFVLAFFGIKKSGPKANGQPYYREER"
     gene            15909..16979
                     /locus_tag="ACZPCL_05295"
     CDS             15909..16979
                     /locus_tag="ACZPCL_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HIR1161927.1"
                     /translation="MKKRNMKMTSVVLAAALAAVSLAGCGGSGKTETTAAAGSADTTA
                     AETGSEAESSEAAGGEAKEGGYKVAMISDTSVSDGGWGASCYQAMVDAAAEKGWETVY
                     TDNVATSDYATVMTDYAELGYDLIFAPGNQYTDAVKQVAEEYPEIKFALLNGTVETDN
                     IQSILPDAEQIGYMAGALAGLMSKTNNIGFIGGMELDTTKAKVECYEKAAQKINPDIK
                     VSSAYAGSFSDTAKGKEIAASMVTTYDVDVMFGDASAVDTGAREALAGQEGRYDIGQP
                     GDLGSADDPVIACSVVTDNAALVKTCMKDIEDGTFGNKTVYGDLSNGCLSVGTFSSIV
                     PEDIQKQYLEAVEQIKAGTFFD"
BASE COUNT         4901 a         3505 c         4126 g         4553 t
ORIGIN      
        1 tgcactctcg caattctaaa aacattcgta atccgctcat ttttcttcaa aaaatggctt
       61 cttactcatg tttttggcgg gtcccaagtt caaaaatcct cctgcaagca agcttgcatc
      121 ggatttatga ccttttgacc ctggtatcat cagatactac agggatttga tcagatgatc
      181 tacgtaaagt tctgcttctc cctggaggaa aggagtctgc atgctttcat aaccttttcc
      241 gaattcatct ttctctacga tataacctgc gctgtagttt gcatagcccc atacgatgcg
      301 cataggagca ttaaagttct ttttgatctc cattccgaag gtggagaaca gctctccggg
      361 gatcgtgatc atctccaggt cgcctaaatg gaagataaca gagcggagcg taacattgat
      421 accgcctgac tggatctttc ttctcatatg gcggacaccg gaccagagaa gctttttatc
      481 atcctctgta acagcagcag ccagcttctt ttcatcttca gctaactgtg ccttcatggt
      541 atccagatca taagtctggt ggatggtgta ttctgcagtt tttacagagc cagcttttaa
      601 ctccatagga gtttctgctt ctgcgaaaac atttatctgc ttcattactt catcacgtac
      661 gcgccacagc tcattttcgt catttccctg acggtactgg cggttgccca tatcaccgca
      721 ggctccctgc atggtaatga aaatggtatt gtatttttca tccagggcct ttcctacatt
      781 tcctaaaaga tcagagctga tcttcatgtt gtggatgcca aggacagttg gatggcaggt
      841 aaactggaac atacctgcaa ctacatggtt ttcattgcgg aacaggatca tgttgatatc
      901 tttatcactt ggtttgtcaa tataattgcg gtttccgtat acaccttcta ttgttccctt
      961 taccatataa ggtgttacct ctaagaagcc acggtcaaag cattcatcta cagcgtcaaa
     1021 aagctgtttc attaccagtt ccctgtaaac tgccttgcga tgatttctgt cttccccttc
     1081 atcttcaaag ctgatatcag ggccagagtg tgtatgaatg gttccgataa tgatattttc
     1141 ctttggaaca gaatattttc cggcaaggac tgtgcgggca taatcagaga tcggttcttc
     1201 taaccggcac agatccaggg tggaccatat aaaaatctcc ccgtcaatgc gcaggactaa
     1261 agtcctgcag taaagttcat ctaaaacacc aatagatttt cctgttctca tggcataacc
     1321 gcgcatgtac ataggaaatg gaggtgtgat attacgtttt gctgcgctta cttcaaccat
     1381 atgataatct ccccttagtt gaataaaaaa tggctctgcc tgccaaataa gacaggcaga
     1441 accgtcatta tggtgatgtc accatcaggc gacatgtccg tttagttatg ttagttataa
     1501 atgatcaagc caggattccc agtgcagagc agatcatgga gaagatgata acaatgagga
     1561 tcagggtagt gatcttcatg cccttctttc caaggagttt atacagtacc aggacagtag
     1621 ctacagagat catagaaggc atgatcttgt ctaacagatc tgcaactgcc agattgactt
     1681 cacccatctg gaatgccaga ggtgtttttg cagttacaac agttgcggac agagcaccta
     1741 caacagtaag gcccagaaca gaagcagcat ctgtgatgcg ggcaagctta ctgcccactt
     1801 tggtgatcag tttggtacct tcacggtatc cgatccacat taactgggta cggatgcaga
     1861 tgaagataag gtttacaatg agccacagga taacaccgat agggttgcct tccagagcca
     1921 tatatcctgc gatggagccc atgatagtag gaagcagtgt ccagaataag gtatcaccga
     1981 taccagctaa aggtcccatt aagctgacct tgatattctg tacagcatcc agggaagtga
     2041 ttccctcttt gtcttccaga gccagagtag caccaagaac gatatttgcc agccatggca
     2101 tggtgttgaa gtatttataa tggttgttta atgcttcaac gtattcgtca tcatctttat
     2161 agatcttacg cagcagaggt ccaataccga aaacaacggc tggagcaagc tgagtatcgt
     2221 agttaaaggt tgttattgcc attaaggacc agcggatagc acctttcaca cagtcttttt
     2281 ttgttaaaac gggagctttt tccattactt cattctcatt attcatcttc aagacctcct
     2341 gcaaatccta cattttcctt tgcttcttca tccatataga acttgtaagc taaagctaca
     2401 ccaaggaatg cgataccgag gatcggtacg cccatgtatg cgctgagtac aaagccgatc
     2461 agaatgtagt tgaagtactt ctttgccggc atgtagtgga gcagcatggc aataccaaga
     2521 ccaggaagaa gctttcctgc tacagacaga ccgcctgtta cccaggaagg aagcacatcc
     2581 aggatagcct gaactaaagg agcgcctgct gttaatgcaa cagcggttgg gaatgccatg
     2641 aatacagcta ccataacagg gcaaaggtaa aggatagcat acattttgcc aaactgtttt
     2701 tcattggcat atttctggga tttctgggca atgaaggagt tgcagatctt atataaaacg
     2761 tccatctgta cgcccagcat accaacagga agaccaatgg tcataccaac ttccggtccc
     2821 tgtccagttg tttttgcaaa tgcgcatgca ataatggttg caacgccgta atcaggtaca
     2881 gaagaaccgc caagagcggc aacacctaag gacatcagtg tgaaagtacc gccgatgtaa
     2941 agaccgctgt tcatgtcgcc taagatcagt ccgcacagga aacctacgat aacaggtctt
     3001 gcatgtgtca gtgataatcc gcggcggtca acgttgatct cagcagccac caggacaagc
     3061 agtacaattt gtaaagcact taattgcata acaatttccc cctttggtta tttcagaaat
     3121 tcgtcaatag aaacttctgc gtctgcaggt acatactgtg catagagctt ggttcccgca
     3181 gcctttaatt tcttgtaagc ctcaatgtcg tccggctttg cgtaagctct ctgggaaagc
     3241 tttacagctt cttcgccttc agcaggcttt acagttccgc caatgaccag ttccgggatc
     3301 tttacgccga tctcagacag cttaaccagt gtttccggct tcttaaccag tacaaataca
     3361 gtgtggtcat tgtattttcc ggctttgaag ttatttaaag ctgtttcctc ggtaatgatg
     3421 gaaatagcca ttccggtagg tctggcaagc ttcatgctgg ctttggtcag ttcattattg
     3481 gcaacagtgt catcaatgat catgatacgc tgtgcaccgg aacgtccggc ccactgtgta
     3541 gccacgatac catgaagaag acggttatca attcttgcaa gtacaatacc catatttacc
     3601 tcctattcgt cctcacggac ggccgggctt tctgcctgcc cggaaagctt attttttgaa
     3661 ttcaaatgca tgtaatcata aatgtaagca atctcactta caggcagagc cacccggtaa
     3721 tgccgggaga tatcctgaaa gctgtccctt acctgcctga taaagtcggc atgtttttgt
     3781 tcaaattctt ccagatcctg ataattatca atgggtgttt ttgtgaccag acgttccacc
     3841 agacaacaca ggtggacata aagcccaatc atgatacgtc ctgcgatctt tcttcctgtg
     3901 atcttctgaa gccgtcccac tgcctgttcc acttcatcta aaagcttgtc aggatttaaa
     3961 atggtaatgg attcaactac attctgcagg gagaaattct ttaccaggtt ctggttaaat
     4021 atttccattt gttcttcatc cagatactct gagaaaatgt ttgtcagctt ttctgcggaa
     4081 ttcatggaaa taatgtcttc cagtgagata aaaggaattc cctcgatctc cggattgaaa
     4141 agcccgatga tgcattttac atggtattgc tggaaaatct catcatggct gccgtttttc
     4201 atcagccggt aataatcata agccacaaat tttacaggaa tggaagtggc ggaagtcttt
     4261 aagatcagct cttttagctt ttccgttgtg tcaatgccgt tttctccgct gaagatcacg
     4321 gcgtcttctt tctgtacatt gtggatgata cggtaggtgc agacattgct ttcgctggcc
     4381 cgtttcagga tgtcttccag cttctggttc ccacagatgc caaggccgat atcaacagct
     4441 aaggcagtgg aaatattgtt gatgatgccg atatttaagt ttcgcaggcc gcccatctgg
     4501 ctgtggatct gctctaaaga acccatatcc accagaagta ctacgtcctg acaggtgata
     4561 aatcgttcaa tgtgtttttc cagcagcccg gtgatgtcac tcatctgcat gtcaagaggc
     4621 atatcaatgg catcgaatac ctttcgccca atgatctggt tagcggcatc tgcaatactg
     4681 gaagctgttg catagccgtg gcttaagata ataccggctg cggaaagtcc ctggatcttc
     4741 tggttctggt tttttatatc taaaagcaaa aacagctggt ttaaactgtc tgcttcaatg
     4801 tccagattca gtttgatcag gtttacgatc ctggaacaga tgactgattc tttttcaaga
     4861 acagcgctta cagcctgaaa catttcttca atctcgtcat ggttttgttt catccagttg
     4921 tcgatcagat ggtcgccctg aagttgtata tataagcggc gggcaagtaa gtggctgtat
     4981 tttttggaaa ggctgatgcc atacatttca cctacatttt caaaaatctg attgatcagc
     5041 tgttcgtaag tggagatctt gatgtttaca agcttctgtc cgtagatcag atagtcgtaa
     5101 taatcattta attgtttccg gaattccatc atcaggccgt caaaactgag catatcctga
     5161 cggtaatctt caaagacatc taacatgatc tggaaatact gtactgtctg ccccaaagag
     5221 gtgtcacggc tgtaactgtt taagtggatc atccgtttgt ctttgtcttc aacctccagt
     5281 tttaaggagg agagcatata ttccggaagg tgataactgt ggatagcgat accatcactg
     5341 gttttttcca gataagctcc ggcacagctg ctggtaacgc aggttttcaa ttcgtcgatg
     5401 ttgttttcgt aggaaaattc cagcatacag tggaaggcgt tctggctgat gcttacatct
     5461 acgcccatgc gccgcccttc tgcttttaag aaggagacta acatttcctc tttttcatcg
     5521 gctgtgcgtt catgcaatgt tgggaccggg atcacaatgg gaatcatcct tgcaagtggc
     5581 ttataaagtg tttccgccgg tacttttgtg gtggaaaata ccagtcttgc attggactca
     5641 aaaacatgtt cgtcatcatg atatccttta tattgtcccg aaattaagta ggaatagatc
     5701 agttcctgtc ctgaaaatgg aagcctgtca atttcatcaa agaaaaggat gccgccgttt
     5761 gcctttgcaa gccagccttt tccgtcttcg ctgccgcaaa gggaaatagc aaatttttct
     5821 tcattctggg catattcgga gcagtctact gtgatgtaag gcgggagaga agctgcgcct
     5881 gcagttccta ttacccgttc atttacagca tattcataca tcagcctgga aagaaaagcc
     5941 tttcctgttc cgccagctcc gttaaacaag gccggaagac catttggcgg gtatttaaca
     6001 gctgctttac actgctcgat gcaggaactt aaactcaggt aataaccgat ggctttctga
     6061 aaatctcttt tattttcatc gcttcggcat ttccgctgaa aatcgtccat accggagaat
     6121 acgcaggttt ccagggcgac ctgggcttcg cgttcaacat ttttcttatg gaagaaataa
     6181 acaggacggg agttgacctt aatggcccgc ccttctttta ccagctcgtt cagatactga
     6241 ctggccagat tacgggaaat attcagtgat tcgctgatat ggttcgccgt aaaatatttc
     6301 agatctttca gggcatagcc ccggcttatt ttttccaggt aaagatagag ttcttctttt
     6361 gtgctgagca tgtacatgat tctcctgttt gttgatttaa cttgaattct tttcgcgcgt
     6421 gttttatgtc tacctgataa caagtgtaat tatacaagaa atagtctgaa aagaaagatg
     6481 ttttgaatga tttcgccagt ttgaaaaggg tttcggcagt agcggtgaga aaaaagggga
     6541 ggtgcccgga gtgggagaat ggggttcgac actaggattg acacttttaa gggggaaata
     6601 tgtgctatac ttaaaatcag atgtcaggat caagatcagg cacagaaaga tcccgggaga
     6661 acattacagg aggaaatcaa tatgcacagt gaaaaaacaa ctatccggct ggtagccgta
     6721 gacatggacg gaactcttct tcatgatgat aagagtatat cagactatac attagatgtt
     6781 ttaagaaaga ttgtaaaaaa aggtgtgatc ctggtacctg cttcaggaag accgatcgga
     6841 ggaatgaaag aagcagtatt aaataatgta gacggcgttc agtatgctat ctgctctaat
     6901 ggtgccgtgc ttatggatgt accggaggaa aaaagtatct gtgaaacagg gatctccaca
     6961 gaaaaagcag tggaagccat tgcgtatctg gaacaattcc cagtggctgt atatgtccat
     7021 acagacagag gtactttccg ggcagaggga tgggagaaaa cagggcttcc tgagaaatat
     7081 ccttatatca ggttcagcga gggaaatgta aaagatctgg gagagttttt aaggaccagc
     7141 ggagtaaaag tgatgaaaat gggtgctttt gtcttaaaag atggactggc acaggaactt
     7201 ctggaaaaag gaagccctat accgggtatc gtatttttgc gcaccggaga tgggatcata
     7261 gaattaaact ctgtggatgc ctcaaaggga aatgcactgt gtacactttg taaaaaattg
     7321 gggataagta tggaaaatgt tctggctatt ggggataacg aaaacgatat ttccatgctt
     7381 caggctgcag gcatctccgt agcaatggaa aacgcagaag atgatgtaaa acaggctgcg
     7441 aaatttgtgg ccgggcataa tgaagaggac ggggcagctc atttcctgga ggaatgggtg
     7501 ctgtaaaata aaacagagaa caaaaaatgg acgcgccggc tgacgcgtcc gtttgctttg
     7561 taaacttgtg atccactgcc tgctgacgca ggcggttgtc agagtattcc tgtagactgt
     7621 aaaaacagta caaacatcat aaccgggacc acatagcgga tcatgacgcg gtacagcttt
     7681 ttacggcgga aagtatcgcc ggtgctttcc atttcttcga tcacatagtc cggtgttttg
     7741 atccagccga tcagaaaggc agagagcagt gcgataaacg gcatcataac gctgttgctg
     7801 atatagtcca tgatgtctaa taactgtccc acagagccgt ttggcagttt tacttcaata
     7861 tagaagatac tgtagccaag ggcaataaca gcagaggcag ccaggtagat cacagtcagc
     7921 acaaggactg ttttcttgcg gcctgtgtgg aagatctcca tgcagttggc agtgatagat
     7981 tccagcacag agatacagga cgtaagagta gcgaaaatag ttgttacaaa gaacaaaaga
     8041 ccaacaaagg tacctgcttt tcccatggcg gcaaatacct taggaaggga aatgaacatt
     8101 aaacttggac ctgcgctcat tccgtcggtg ccggagaaaa caaaaactgc cgggatgatc
     8161 atggcgcctg ctaaaagggc aacgcctgtg tcgaagatct caatctgatt aacagcttta
     8221 tttaagttaa cttccggttt cacataagag ccataggtga tcatgatacc catggaaaca
     8281 cttaaggaaa agaacagctg gctcatggca tcaagtaaga tctgaaggaa gcggctaaag
     8341 gtcagtccct caaaatgagg ggttatataa taaagaaggc cgtccatacc ggtacgcatc
     8401 tgtccgcttt cgtcaacatg gtgcagtgtc agggaatagc ctgcgatcac aaccaccaga
     8461 accagaagta tgggcatcat gcaacgggaa acacgttcaa taccgccttc cacaccattg
     8521 tatacaataa acgctgttac gcccataaat aaaagggcaa acaggacagg tgatacaggt
     8581 gaagtgataa atgaggtaaa ataattgtcc tgggctgcag cggcagtctg tccggtgaga
     8641 tagacaactg cgtattttgt gacccagccg ccgataacag catagtaggt catgatcagg
     8701 actggaacaa gaaaggttaa aattcctaga aatttccatt taggcgacat ctgggtataa
     8761 gccccgatag cgctttcttt tgttctgcgg ccaatggcaa tgtcagatgc cagcagtgtg
     8821 aatccaaatg ttaagaccag tgccagatag atgactaaga acagtcctcc gccgtccttt
     8881 gccgccagat acggaaaacg ccacaggttt cccacgccta cggcactgcc ggcagcagct
     8941 aaaacaaaac ctaactggcc ggaaaaatta cttttcttct tattcatgaa aaagaatccc
     9001 cctaaaatta tctatagctg aacttgcttg tcataccaga atagcacatg ttataatatc
     9061 agtaaaacaa atgtttttat gactatatat aattttttgt aatggatatg gggctataga
     9121 taaatttatg aaagaacaga aagacagata tgagatattt ttaaaggtgt gcgagaccgg
     9181 cagcttttcc ggggcggcag aggcattaaa ttacacacag tccggcatca gccagatgat
     9241 ggctggactg gaagaagaac tggaagtccg gctgtttgcc agagtaaaaa agggagtcat
     9301 acttacagac aacggagcca gactgattcc ttatattcag gaaatggtaa accagaaggc
     9361 aaagctgcgc caggcggcct ttaatattaa tcataagatc gaaggaaagc tgcgggtagg
     9421 aagcttttcc agtattacag cgttgtggat gccgggagtg atccatttct tcaaagaaca
     9481 ttatccaaag gtgcaggtag agatctttga cggcaattac gatgagatcc gtgactggat
     9541 catccacgga caggtggact gcggtttcct gtcatctatt gtggcagacg acctgaaatt
     9601 ttatccactg atggatgacc cgctgtgtgc agtgatgcct aaggggcatc cacttacaga
     9661 aaaagaacgg gtcacagtgt cagaactgtt ttcgtatccc ttaattatag aaacacctgg
     9721 ctgcgacaat gatatccagc atttattagc aaaatgttcc gtaaagccga agatcaccta
     9781 cagcttccgg gacgatacac tgatcatggc ctttgtaaaa agcggattag gcgtcaccat
     9841 ttcccagaaa atggtactaa aagcctttgc cagagacgat gtagaatcca ggcccttaga
     9901 accagcttca caccggaccc tgggactggc attttcaaag acagccaatt ccgtggtgtc
     9961 ggggataatg ttggagtatt taaagaaaat gacgggataa atcataagcc agattgaaaa
    10021 ttcagccaat taaaaccgat gaaaacttgc ctaacttctc taaagttgct ataattcttg
    10081 taaccgaagg cgtgataatc tttctcaaaa gcggggaaga tgcaagtata tgtacacggc
    10141 gtaggtgtgc atatagcaag taaggagaag tcgtgcgtac tttaaagaaa tccatatgga
    10201 gttatatgta taaggtcaca tttgcgttgg taagtgtgat ccttattagc attacggcat
    10261 tgaatatagc aaatgagcag agccgggcgc aggggacggc ggataagatc tttgagcaga
    10321 tggataagat gctggaggag aatcagaagg aacttaccag gctgcgccag gaatatgctg
    10381 cgaaatgtct tcataataca gaggccattg cctatattct ggagaagaac accggggcaa
    10441 gaaatgatct gtatgaactg cgtaagatcg cggaatttat ggaagtagat gagatccaca
    10501 ttattgatgc tgcaggcgag atcgtttccg gaacccatcc ccagtattat ggttatagct
    10561 ttgactccgg ggagcagatg aattatttta agcctatgct taaggacaaa tccctgaaaa
    10621 tggttcagga tatagagccg aatacagcag agcataagct gatgcagtat tcagctatgt
    10681 ggaacagcac cggggaattt atcgtggaag cgggaattga accggtaaat gtatccaaag
    10741 taacagaaaa aaatgaactg ccttatattt tttcccagct ttgtgtcaac ccggatacca
    10801 gctactttgc ggtaaatgca gaaacagaaa agatcgtagg tgctacggac acagaattag
    10861 tgggaatgga catgaaggaa attggtttaa atacggaaaa gatccatagt acaaaaggtt
    10921 tttccggtat gatcaatgga aaacgttcct atatcgtatt taagaaggta gcagataatt
    10981 atatcggtgt ggcagtgacc agtaaggcac tttatgaacg cataccgtta aatatgctgt
    11041 atctggcggt atgtcttgta ctcattgcgc tggcattgtt taaggcagtg acccggtatc
    11101 tggaccggga ggtagtccag aagatcgggg aagtaaacgg aaagttgcat aagataacca
    11161 tgggtgactt aaacgtcaag attgatgtcc acagcagcag ggaatttttt gaactgagcc
    11221 gttatattaa tgaaatgctc cagagcctgt tagtcaatga caggaaaatg acatatgtgc
    11281 ttggaaagac ggatatgcac attggtgtat atgagtacaa ccggcaaatg aagagagtcc
    11341 gttttacagg tgatattctt cagatcttaa agaccggcac agaagctgcc tggaagctgc
    11401 cttctgactg ggaagatttc aagaaatata ttagaaccat gcaaaaagag ccggttgcag
    11461 gtgaaaatga gatatttgct gtaggaaatg gtaaatattt aaaaattgaa gagatccagg
    11521 agggcgaaga gatcttcggt gttattacgg acgttacaga ggagatccgc aagcgcaggg
    11581 agatcgaata ccagagagat catgaccagc tgacggggct gtttaaccgg actggcagag
    11641 ataccaggct ttccaaaata ctggaagaat gcagggaacc atattactgc gctgctgtta
    11701 tggtagatgc tgacggtctg aaaatggtta atgacactta cggacatgaa aatggtgatg
    11761 agtaccttaa aaggattgca gaaatgctga aacaggaagc aggtgaaaac agcattctct
    11821 gccgtcaggg cggtgatgaa tttacattgt tctattataa gtatgggaaa gaagcgcttg
    11881 tgcagaaaat agaagccctg aaggcgcttc agagcggaca gaaggtccgg ctgcgggacg
    11941 gacttaccgt tgatctgagg ttctctatgg gaagcagcat ggcttacggc gcagtagatt
    12001 atgataaaat gtaccgtgaa gcagatgaaa aaatgtatga agataaaaga atgcggaaaa
    12061 agctggcaga agagacaaat acaatagaat cttaggaaaa ttcagaaaaa atgaaagaat
    12121 tatcccatca tggtaaccct gaaaaccatg atgggataat tttattaaga aaaagaggga
    12181 ataattgaca tcaggaatct gtctgtttat aatgtaggtc agtttacagg tcataggatt
    12241 tacaagggag tgaatcgctg aaagacatgt tctttcagca tgttcatttc ctttttttat
    12301 ttataagcat tgaaattgaa aaggaggggg atgaatatgg cggagctttt aagaatggaa
    12361 gggatcacca agcagtttgg tgcagtctat gccaataagg atatcaatct gtctattgaa
    12421 gaaggcgaaa tccatacact tctgggtgaa aatggtgcag gaaaaagtac cctgatgaat
    12481 attctcatcg ggctgtatca gcctacttcc ggtacgatct ggttaaaagg caaaaaggta
    12541 aagatcgatt caccaaaaac agcagtaaaa ctggggatcg gaatggtgca tcagcatttt
    12601 atgctggtgg atgccatgac agtatttgaa aatattatcc ttggaacgaa aaaggacagt
    12661 tccattttca ttaaaaaaga agaacggaaa aaggaaatcc tggagctttc acaaaaatac
    12721 ggactggaag tagatctgga taagcaggta aatgagattt ctgtaggcga acagcagcgt
    12781 gtagagattt taaaggctct ttaccgggga gcggagcttc tgatcttaga tgagccaacg
    12841 gcagcgctga cagaccagga agtagaaggt cttttctcta ttatgggaag actgacggca
    12901 gaaaacaagt ctgttatttt tatctcccac aaaatgcgtg aggtaatggc agtcagtgac
    12961 cgggtgacga tcctgcgggc tggtcagact attactacag tggataaaaa ggatacagat
    13021 ggggcacagc ttgcaaatct gatgatcggc catgagatga cagtcagtgc ttataaaaaa
    13081 gtgacagaac cgggagaaga tgtgctgcgt ctgctccatg tggattatca gaaggatcat
    13141 aaacacagcg gtttaaataa cgtaagcttc tccataggaa agggagaaat cctgggagtg
    13201 gccggagtag acggcaacgg acagtcacag cttgcgcagc tggtaacggg agttatttca
    13261 ccagacagcg gagaagtgga attaaattcc cacaaagttg cccagtttgc gcctaatggc
    13321 ttcattcttt ccaatgtatc ccatgtcccg gaagaccgaa acaaaatggg tctggtagga
    13381 aatatgacag tagaagaaaa tctggtttta aaggccacgg aagaacctcg tttttcctat
    13441 gcacacggcg ctttattaaa gaaaaaagcg atccggaagt ttgccctgga acttcagaaa
    13501 aagaatgata tccgctgtgc ttctattgag caggaagcaa gaaacttatc cggcggcaat
    13561 cagcagaaga tcatcttagc cagggaaatg gaaaatcacc cggaactttt agtagcagtc
    13621 catccaacca gaggtctgga catcggggct tcccagtatg tacacgatac catgatcgaa
    13681 gcaagggaca agggctgtgg gatcttattg atcagtgcgg attttgatga agtcctaaaa
    13741 ctttcagacc ggatcctggt tatgtttgaa ggccaggtca tgggaattta ttctggtgaa
    13801 aatccgccgg tagaagaaat atccctggca atggcaggaa aggaggaata gggaaatgaa
    13861 attaaaagat aatctggcaa agatcctgat ccccattctg tcgatcctga tcgcatttat
    13921 agcaggaggg atcatcatcc tgtgtctggg gaaaaatccg gtggaagctt atggatacct
    13981 gttttccggt gcatttggaa gcggacgtaa gatggggcag actttagtaa ttgcatgtcc
    14041 gctgatcttt accggacttg cagcagcctt tgcctataag tgcggcgttt ttaacctggg
    14101 cggtgaaggg cagtttatta tgggtgcagt taccagtatc tttgtctctg caaaactggg
    14161 tgacgcagga atgggcggac ttcttgtaag ccttcttgca ggaaccgttg cagggggcat
    14221 ctggggcgct attcctggta ttttaaagat caccagaggc ttaaatgaaa tgatcgtcag
    14281 catcatgctc aactatgtgg caacgctgtt tatgggatat gtgtatacaa cccttttgcg
    14341 tgacggcagt gttccacaga cggttgcagt ggcagacagt atgaagattg ccggtatttc
    14401 aaaaggattt ccagcccatt ggggtgttct ggcggcgttt ttgcttgcag cggttttata
    14461 ctatttcatt ttttatacgt ccaaaggatt taagctgcgg gcagtgggaa tgaacgcaac
    14521 agcggcattt ttcaatggtt ttcccgttaa taaatacatc ctgttttctt ttatcacttc
    14581 aggcgctatt gcaggacttg gaggaagcat agaacttcat ggaaaacagt tccggcttat
    14641 gagcggtttt ggctccggct ttggtttcga tggcgtggca attgcactga tcgcccagct
    14701 aaatccattg ggaacggcta ttgtggcctg cattttcgca gtgctgcgaa agggcgcaag
    14761 taccttgcag acagccacgc aggtaccaag ctctgtggtg gatatcattc aggctctggt
    14821 cattatcttt gcagtagccg gaaccgcatt attaaaactt ccgacgatca agcagttcct
    14881 tataaaccat ggaaagagaa aggaggcgca gggctgatgg catcattttt tacaatgaat
    14941 tttctcacag agcttctgct ctcaacggtg cgcatggcaa cgcccatcct cttggtggct
    15001 cttgcggaaa cctattcaga gcgtgccgga atggtcaata tcggtctgga cggcattatg
    15061 gccattggtg ctttggtggg ctttctggtg ggatatgaaa caggaagtcc ctgggcggga
    15121 gttttagcag gcgctctttc cggtattgct accaatatga tctacgcttt ctgcaccatt
    15181 accctttgcg cggaacagac cgtttatggt atggcgatca atatttttgc tccggcgctg
    15241 gcatctttta tttaccggct ttccttttct gatacgtcta cactgatcca gggcgtgtct
    15301 atggggatca tgcccatccc ggtactcagc aagatcccga tgatcggaag tatcttcttc
    15361 cagcagagtc ctcttgtgta tggggcatat cttctggttg ccgtgacagc ggtattttta
    15421 aaccgtacaa aggcgggact gaatttcaag gctgtaggtg agttcccaaa ggcagcggaa
    15481 acactgggaa ttaatgtagt tctccagaaa tacattgcct gcatcatatg cggtgctctg
    15541 gccggtatcg gcggtgcata tctgaccatc tgttatacaa gtacctattc agaaggcatt
    15601 gtggcaggcc gcggatttat tgccttatca gcagttatct ttggacggtg gatgccaaca
    15661 ggagttatgt ttgcatgtct gctttttggc ttctgtgatg cgttgcagat ccgcttacag
    15721 ctgttaagtc cgtccactcc ttatcagatc cttcagatga tcccgtatct gtgtaccctg
    15781 tttgtacttg cattttttgg cattaaaaag agcggtccga aggcaaatgg acagccgtat
    15841 tacagggaag aacgataaga aaaacgatga aaaagtaaaa attattttat atataggagg
    15901 aatagattat gaaaaaacgc aacatgaaaa tgacatctgt agttttggcg gcagctcttg
    15961 ctgcagtaag ccttgcaggc tgtggcggtt ctggaaaaac agagacaaca gcagctgcag
    16021 gatccgcaga cacaacagcg gcagaaactg gttctgaagc tgaaagcagc gaagcagcag
    16081 gcggggaagc aaaggaaggc ggctacaagg tagctatgat ctctgacacc tctgtatcag
    16141 atggtggatg gggtgcaagc tgctaccagg caatggtaga tgcagcagct gaaaaaggct
    16201 gggaaaccgt ttatacagac aatgtggcaa cctctgatta tgccactgtt atgactgatt
    16261 atgcagaact gggatatgac ctgatctttg cgccaggcaa ccagtataca gatgcagtaa
    16321 agcaggtggc agaggaatac ccggagatta aatttgcact gttaaatggt actgttgaga
    16381 ctgacaatat ccagtctatc cttccagatg cagagcagat cggttacatg gcaggtgctc
    16441 tggcaggact gatgtccaag accaacaata tcggatttat cggcggcatg gagctggata
    16501 ccacaaaagc aaaggtggaa tgctatgaaa aagcagccca gaagatcaat cctgacatca
    16561 aggtatcttc cgcttatgca ggctctttca gtgatactgc aaagggcaaa gagatcgcag
    16621 catctatggt gacaacttat gatgtagatg tcatgtttgg tgatgcgtct gcagtagata
    16681 ccggtgcaag agaagcgctg gcgggccagg aaggcagata tgatattgga cagcctggag
    16741 atttaggttc tgctgatgat ccggtgatcg catgctctgt tgtaacagac aatgcagcac
    16801 tggtaaagac ctgtatgaag gatattgaag acggaacttt cggaaataag acagtttatg
    16861 gcgatctgag caatggctgc ttaagtgtag gtaccttctc ttccatcgtt ccggaagaca
    16921 tccagaaaca gtacttagag gcagttgagc agatcaaggc tggaaccttc tttgattaaa
    16981 tatcagtaac taaaacttcc cacaccgatg tggtgtgttg aaccttgaaa attcaatatt
    17041 tcagccaata aaaaaggcat aaacctgttc cgtttggtat aatgg
//
LOCUS       DBMDUA010000045        16663 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1985_length_16663_cov_2.759449, whole genome shotgun sequence.
ACCESSION   DBMDUA010000045 DBMDUA010000000
VERSION     DBMDUA010000045.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 16663)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16663)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16663
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1985_length_16663_cov_2.759449"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(343..1461)
                     /locus_tag="ACZPCL_05300"
     CDS             complement(343..1461)
                     /locus_tag="ACZPCL_05300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1161928.1"
                     /translation="MKLGILCTMINGFGRRGYYNSQEIGLGRALARKGHEVMIYKGID
                     PSEKEEKVQVEKNLTIWYLPMKHLGAHGFMDCKNLDPQLKALFCFGDQQIFLPHVYRW
                     CRRRRIPFVAYVGTAHSLDSNFKSKVMNALFAAGTLRIYKKNPVLAKTSAAKEELRQL
                     GIPHAVIAPVGLDTAVLKKNIPAVEKMRLRKEHGFEADDVILCNVSRLSWEKRPLELI
                     DLFLQVKGKKKFKLIIVGNGPLEEELNEKIRKNGLEKEVKIYPNVPYEKMWEIYEMSD
                     HYLNMNKGEIFGMAIMEAVYYKTSVAAIRALGPSVTLKDMKGHKLCENDDEMAEWITG
                     PYPSEKDLQESSEKMAATFTWDRCADAFLQASSEKSSR"
     gene            complement(1503..2249)
                     /locus_tag="ACZPCL_05305"
     CDS             complement(1503..2249)
                     /locus_tag="ACZPCL_05305"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008194 - UDP-glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HIR1161929.1"
                     /translation="MEKQRLLNVYVNNLTMMESVNRIHDMIREKQTSYVVEVNVDVVM
                     KMEKDPYLKKISDEADLTLVDGQPLVWISKMKGNTPIREKISGSDLVPELLDELSREN
                     RSVFILGGLGDTAKKAAKNVKKHYPGMSIAGTYAPPLGFEKDAKEIEKINHLVAKTHP
                     DLLLACFGCPKQEKWIYENYKKCGAIVSVCAGATVDFLAGNIKRAPRWISQCGLEWFY
                     RFLKEPRRLFKRYFIDDMGIVKLVWKYRSL"
     gene            complement(2281..3813)
                     /locus_tag="ACZPCL_05310"
     CDS             complement(2281..3813)
                     /locus_tag="ACZPCL_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014051.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR1161930.1"
                     /translation="MNNQKQETSMKRAIMMNMFWALFSLLINTLMNFLITPYVTNNIG
                     VEAYGFVALANTFISYVDVISVSLNAYAGRFISVAYHKGQKEEANRYFSSTIVADAVL
                     GGTVFLLGIAMILKITWFCKIPQELVPDVKRLFFLVLLRYIMVVMRTAFETAAFIADR
                     IDITERLQSGAYLLQAGILLFVCLNWFPHVWYVGLASMSAALFLLLANVAVSRKLLPD
                     LRFGRDKWSPGAIWEIVSTGIWTSINNLGNVLNNGLDLWITNRMLDAVVLGQISVVKT
                     IAFLSNSVILKVSSSFKPRLLMLYAEKRMSQLTELLKKAMRCTGFFEAMVIGLFSVSG
                     YDFFRIWLPGQNTKFIFWGAMIVLASDLVPGTVNPLYYVYTLTKRLKVPSIVTILMGV
                     ANVISMILLLKWTSAGGYAVLLTTLVINGVHFFDAPLYAAHCLGLSKKEFYPTIIRNV
                     LSAVTVTGIGHFLRLHWPFVSTWMGLFLKTCLAGVILMPLLCVLLFPASDLKEFLWKN
                     KR"
     gene            complement(3810..4916)
                     /locus_tag="ACZPCL_05315"
     CDS             complement(3810..4916)
                     /locus_tag="ACZPCL_05315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR1161931.1"
                     /translation="MKVLMVDQFLPNSVYVVELCRELRKYAKITIFCKKNAGTDLDGV
                     IWKDKLYAGGKGKMAAVWEYGKGLFRLQKEIKKGSYDVVHVQSFKDAKYEIPLYCKNK
                     KYIPRLVHTVHNLLPHEASAGDRELYSRFYNCCDLLVVHNEYCRQLLMKEYQIPEEKI
                     CITPHGSYTQIRKEENTDFGLTDRKIRFLQFGILRPYKGVDILLKALAQIPAEKREKI
                     QVIIAGAQHPKLDPTDYGAMIRDLGLEDTVDLLKKHIPDEELDALYRQADICLFPYRD
                     IYGSGALLMAYSYGKPVITSDIPAFREETQNGKTGILTDGEDPEKLKEAILQAADWSR
                     EEYLDDQKEIEKLTEEKYSWKISAKILWEAYRKR"
     gene            complement(4913..5605)
                     /locus_tag="ACZPCL_05320"
     CDS             complement(4913..5605)
                     /locus_tag="ACZPCL_05320"
                     /inference="COORDINATES: protein motif:HMM:NF016763.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR1161932.1"
                     /translation="MLSSLDMRDGMQLFLCAVILFGAYCAALGVISGRIQNRSGIVPA
                     AMILLCIFGCVGVMLFFVFQVTGESSIVLFMLLVLLSLLIFGGELWFIMQNIRTLNKG
                     GCALLLTYVLAVLAVTLLWRKGGTDSRLQMQLFTGVGEALQKHSLQPVWHMLQNVALF
                     VPVGLLFPLIHPAFDHPGYAVMNGLMLSVWIETIQMITASGTCDVNDILTNTLGTAMG
                     YGTFWIGRGMRK"
     gene            complement(5595..6914)
                     /locus_tag="ACZPCL_05325"
     CDS             complement(5595..6914)
                     /locus_tag="ACZPCL_05325"
                     /inference="COORDINATES: protein motif:HMM:NF016800.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR1161933.1"
                     /translation="MRIQNSQRQTLSSQSSRKDLLKSRIVDTTVIFILLVSFGFPGKY
                     TMLLGSASSKILEYASFGAQRLLMLFSSSEDGFMSLKLLDLKEKYAVIYFMAAVYFLD
                     SMLVTRYPSEQLISCIRYTATIFFALWMVEYYDGEKLFHLIAAAQVIFTCLTFAFMLL
                     RPGAVFSQEQGEHDFTGFLRTKNNAASQFSICILLQIAWFRILRAKQMIVSRKYLLSL
                     LAQGIFLVLCNAKGAVLCLIFPLLYVLFGKYKEEPGKRLPLGVIYVTLSVAFLITALT
                     ILPLFQPLFSLIGKDATLTGRIPLWRQIIQVMINRHTFTGYGYGMFWRDRQAVAMVHQ
                     AFSRNSFMGSMTTGAHNVLLELWMNVGLFGVAAYFTAMIISTIGAERMEWTGYLFSAA
                     YILWFMVFGWTERSMSTYEYQTLFLFIAMGSACNKQRVGPKRRQYVK"
     gene            complement(6862..8583)
                     /locus_tag="ACZPCL_05330"
     CDS             complement(6862..8583)
                     /locus_tag="ACZPCL_05330"
                     /inference="COORDINATES: protein motif:HMM:NF022077.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR1161934.1"
                     /translation="MNGVQIRVGDLLYAIGKRWKMILAFVLVGCGFGAALSGISYVQG
                     NYTNYRINCSIAVTSQSETGNFTGNSSYFNPNDFYLAQDMVDAVGYVMKSERVLREAA
                     ESVRIVDPDPVAIARNLDIERYNETQIIEISLKWHDADTGIRLMNAILDTSREVLPKT
                     LMVGSVSTIDEPQAEYIMGMGGLSHIWLITGVMGFIMGAGMAMLELIMRPTLLNLKDV
                     ENVFGLETMGIIPNDQEYFRDGRVILEKDGMTDSAVRQNFTSAAYILQNRLGLKDETC
                     SFYVTSAEEGEGKSTVAANLAVQLSDMEKKVLLVDLNTRNPRLGSMFLHSVDYSRTLN
                     ALYKGETDPLEAVISLTGYLDLLPSVLERNAIPMDGQVFDFIRGLEKDYDYVIIDTPS
                     VGQVSDVLSLNQIAQAVLFVIRYDDVALPMIQNAIDKLDKSGIRILGCVVNAAQKTGG
                     KYFGGERNVVENRGRKPKKPEAETMKETMDDLNDLEDMMKSAKETQALKSTHPHTEED
                     TAFNGLLEELTDDLLETSNRLSNQEAIEALVQMGMNESGREESQEELENEDTEQSETD
                     PEQSEQP"
     gene            complement(8580..10250)
                     /locus_tag="ACZPCL_05335"
     CDS             complement(8580..10250)
                     /locus_tag="ACZPCL_05335"
                     /inference="COORDINATES: protein motif:HMM:NF037639.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family protein"
                     /protein_id="HIR1161935.1"
                     /translation="MRRRRKDQNGEYNYWQPATDMMTGLVFVLMLIVALLGLYLLSDY
                     TGYKYDETEGVEETTAQETRPDDDGWSWKEYKGNGDHEDVGDGAGDGGGSGQEQPIIQ
                     TGGGGYGDEGIKSAVFTELVDDETDRIIPDEGVRFELYRTDWGLQNGMGALQILNTYY
                     PEKISYREYETTEEGVFYLPEKIYQGDYFFRELNEPEGYDAAGDTYFNLDKMYDWTDP
                     FVVQIRVSPSKNVIRVQMSDLLSKDPVGGGTFQVRAAEDVATLDGTVRYIAGQLVDTI
                     TCDENGYGESKELYLGSYTLEQAGIPRYYAGINEQPEALVEKKNGEDPPIHEIDTEKT
                     KITLKLSDELYQQQPLSGAVFTVTNGTTGVSQTVETDQNGQILLTDLDKNTSYKIRQQ
                     QTIEDYQLDPIEYTEDVDAYGRINGENQAEMDLTNRMLRTSVHGTDMILRKDVADIQL
                     SLYDSQDQLIESWVSGKSGKMFTNLKEGSYYVLQGDDGEKRYDFTVADTAQVQNWNVT
                     VLTLRGILTLAAGMVGGTVVIILLLFAGITLAGRRKKEDKKKDSEKKR"
     gene            complement(10247..11812)
                     /locus_tag="ACZPCL_05340"
     CDS             complement(10247..11812)
                     /locus_tag="ACZPCL_05340"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR1161936.1"
                     /translation="MKRLMPIFIVILLSGCGILTAYAPNMLSMIVVAVMGVVVLLGII
                     YGLIPVLTFTQGLLTGQKSIQRASEAEEKSVWITALQMDRFFRQKKMDQLFREYRDKV
                     KQQRQNGQIVSDLEDILNEDVLALYSWQGVVAQIPGTLTGLGILGTFIGLLRGLRNIN
                     FVTVEAALSSVQSILTGIDTAFYTSIAGVILSILFNISNNILRNTMDREMGIFLEVFH
                     RVVLPTTQEQERYRSRRETRQIMDLLDRLPKKDDYSASGMGATADSAIGNERLLMPQI
                     LNGLKKNEFIFLLQPQYELNTRKIIGAEALVRWNHPALGEISPAVFIPVLENNGYITK
                     LDQKIWEDVCAMIRRWIDEGIKPLAISINVTKTDLLAMDVVEIFDELLKKYRIPPKYL
                     NIDIAENAYKEVYGIVSEVEKSLQQKGFRIALDGFDGDYMGLHSVGEIGADVLKLDLR
                     SLNKEKKSGSVQDVFDRAKTLRLTLTAEGIESMEQLSLLRKCGCTEGQGYVFSRPLSI
                     EEFEKILKVGQDA"
     regulatory      11945..12020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            12528..12599
                     /locus_tag="ACZPCL_05345"
     tRNA            12528..12599
                     /locus_tag="ACZPCL_05345"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12561..12563,aa:Glu,seq:ctc)
     gene            complement(12814..13281)
                     /gene="smpB"
                     /locus_tag="ACZPCL_05350"
     CDS             complement(12814..13281)
                     /gene="smpB"
                     /locus_tag="ACZPCL_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR1161937.1"
                     /translation="MSKESIKLIANNKKAYHDYFIDDKWEAGIELFGTEVKSIRMGKC
                     SIKEAFVKIDKGEVYVYGMHISPYEKGNIFNKDPLRVRKLLLHKYEIMKLNGKIAEKG
                     YTLMPLQVYFKGSLVKVQVGLARGKKLYDKRADIAKKDQRRELEKDFKVKNLY"
     gene            complement(13370..15520)
                     /gene="rnr"
                     /locus_tag="ACZPCL_05355"
     CDS             complement(13370..15520)
                     /gene="rnr"
                     /locus_tag="ACZPCL_05355"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIR1161938.1"
                     /translation="MLTELVHDKAYVPMKAKEIAMLLNIPKAMRGELQEVLDELVSEG
                     KLGISKKGKYGKPETFAVVGTFTGHARGFGFVTVEGMEQDVFIPEDKTGSALNGDKVQ
                     IVIESEGRNGRRAEGTVLKVLEHANSQLVGYYQKNKNFGFVIPDDQKIAKDIFIPQGC
                     DMGAVTGHKVVVKVTDFGDERKKPEGRIKEILGHVNDPGTDILSLVRAYNLPEVFPEE
                     VMKELEHVPDSLPTEGLKDLPEGFEGRKDLRDLQTVTIDGEDAKDLDDAVTLELLENG
                     NYRLGVHIADVSHYVKEGSELDKEALKRSTSVYLVDRVIPMLPHKLSNGICSLNQGTD
                     RLAMSCLMEVDPHGTVVDHEICESVIRSDRRMTYTAVNAILSEADTKEKEEQKAGLLE
                     EYKDFVAMFERMKELSAILREKRSKRGAIDFDFPESKIILDEKGKPVDIHPYERNVAT
                     KIIEDFMLLANETVAEDYFWQSVPFLYRTHDDPDPEKMKQLGVFINNFGYSLRLTNGE
                     VHPKELQKLLDKIEGKLEEPLLARLVLRSMKQAKYTTQCTGHFGLAARYYTHFTSPIR
                     RYPDLQIHRIIKECLRGGLKEKRVAHYEKLLPQVAVQTSALERRADEAERETEKMKKC
                     EYMSRFVGQTFEGVISGVTNWGFYVELPNTVEGLVRISELRDDYYIFDESRYELVGEM
                     SRKVYKLGQTVRVVVAGTDKLLRTVDFVLENQAV"
     gene            complement(15830..16081)
                     /gene="secG"
                     /locus_tag="ACZPCL_05360"
     CDS             complement(15830..16081)
                     /gene="secG"
                     /locus_tag="ACZPCL_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR1161939.1"
                     /translation="MAVVKVLLSIIYVVLGIAISVVILMQEGKSNGLGSAIGGMADSY
                     WSKNKGRSMEGALEHFTRYGAIVFMILTLVLNILMKTQG"
     gene            complement(16261..>16663)
                     /locus_tag="ACZPCL_05365"
     CDS             complement(16261..>16663)
                     /locus_tag="ACZPCL_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161940.1"
                     /translation="IYTVKENSFNFSEKIPKEGTYYFKVRAVDSRGNQGDWEESAWFE
                     VTKEQMKDWKGHWENNEKGWWYVNRDGSYPVNNWQQIDSLWYFFDEEGYMKTGWIHWN
                     EKDYFCDESGAMLVNTVTPDGASVGADGAKL"
BASE COUNT         4292 a         4382 c         3097 g         4892 t
ORIGIN      
        1 acttcgcata catctttaca gactcattag aaattgtacc cttttcatca tctgcggaag
       61 tagtaattcc tacccagata tcatcataca ctactttacc agagattttt gcatcatcca
      121 tagttgaaaa ctcggtataa gtgtttccag gtttatcagt tttaggacta ccgctttaac
      181 tggtcattgg taagcagtcc cagttcgatc agcatatcgc ccaatcgttt tgtacgcata
      241 tcttcctttt atcctttctc atttgttcta aatccatgca agtacatctc tgaaactgat
      301 tatactctgc ttttcccata ctggcaaata ctgttccggt ttttacctgc tgcttttttc
      361 agaagatgcc tgtaaaaagg catctgcgca gcggtcccag gtaaatgtag ccgccatttt
      421 ttcagagctt tcctgcagat ccttttccga aggatagggg ccggtgatcc attctgccat
      481 ctcatcatca ttttcgcaga gcttgtgtcc cttcatatct tttaatgtta cagaaggtcc
      541 cagggcgcgg atagcagcca cagaggtttt ataataaacc gcctccatga tcgccatacc
      601 gaagatctcc cctttgttca tgttcagata atgatcggac atttcataga tctcccacat
      661 cttctcataa ggaacattgg gatagatctt tacttctttt tccagaccgt ttttgcggat
      721 cttttcattc agttcttctt ccagcggacc gtttcccaca atgatcagct taaatttctt
      781 cttacctttt acctgcaaaa acagatcaat cagctctaaa ggacgttttt cccaggataa
      841 gcgggaaaca ttgcataaga tcacgtcatc tgcttcaaat ccatgttctt ttctcaggcg
      901 cattttctca accgccggga tatttttctt taaaacagcg gtatccagtc ccaccggtgc
      961 gatcactgcg tgagggatac ccagctgcct taattcctct tttgcagcgc ttgtctttgc
     1021 caggaccgga tttttcttat agattcttaa ggtacctgct gcaaaaaggg cattcatcac
     1081 cttgctttta aaattggagt ccaggctgtg ggcggttccc acataggcca caaagggaat
     1141 gcggcggcgc ctgcaccatc tgtatacatg aggcaggaag atctgctggt ctccgaagca
     1201 gaacagggct tttaactgcg ggtccagatt tttacagtcc ataaagccgt gcgctcccag
     1261 atgcttcatg ggaagatacc agatggtcag atttttttcc acctggacct tttcctcttt
     1321 ttctgagggg tcgatcccct tatagatcat cacttcatgg ccttttcgtg ccagcgcccg
     1381 tcctaaaccg atctcctggc tgttgtagta tcctcttctg ccaaatccat tgatcatggt
     1441 acataaaatt ccaagtttca tctgttttcc tccacccatc tttctcatct tccggaaatc
     1501 atttataaac tccggtattt ccataccagc tttacaattc ccatatcgtc aatgaaatat
     1561 cgtttaaaaa gacggcgggg ttcttttaag aagcggtaaa accattccag gccacactgg
     1621 ctgatccatc ttggcgcccg tttgatattt cctgccaaaa aatctacagt cgcccctgca
     1681 catacggata caatggcgcc gcatttttta taattttcat agatccattt ttcctgtttt
     1741 ggacatccaa agcaggcaag gagcaggtcc ggatgggtct ttgctaccag atggttgatc
     1801 ttttctattt ctttggcatc tttttcaaag cctaacggcg gggcgtaggt tccggcaatg
     1861 ctcattcccg gataatgctt tttcacgttt tttgctgctt ttttcgcagt atctcccaga
     1921 cctcccagga taaatacact tctgttttcc ctggaaagtt cgtcaagcag ctccggtacc
     1981 agatcggaac cggagatctt ttcccggata ggggtatttc ctttcatttt ggaaatccag
     2041 actaagggct ggccgtctac cagcgtcaga tccgcttcat cggagatctt ctttaaatag
     2101 ggatcttttt ccattttcat tacaacatct acatttactt ctacaacgta ggaagtctgt
     2161 ttttcacgga tcatatcatg gatccggttt actgactcca tcatagtaag gttatttaca
     2221 taaacattca gcagtctttg tttttccatc ggtgtttccc tctatgttta tattttgtgt
     2281 ttatctttta tttttccata aaaattcttt cagatccgat gccggaaaca ggagcacaca
     2341 caaaagtggc atcaatatca ctcctgccag acatgttttt aaaaataacc ccatccatgt
     2401 tgaaacaaaa ggccagtgca ggcgcaggaa atgaccgatc cccgttacgg tcactgcaga
     2461 cagcacattc cgtatgattg ttggataaaa ttctttcttt gaaagtccca ggcaatgggc
     2521 ggcataaaga ggagcatcaa aaaaatgcac tccgttgatc accagtgtgg tcaaaagaac
     2581 ggcataccct cctgccgacg tccactttaa taaaaggatc atggagatca catttgccac
     2641 tcccataaga atagttacaa tgcttggaac tttcaggcgc ttggtcaatg tgtatacata
     2701 atacagcgga ttgacggttc ccggtaccag atcgcttgcc agaacgatca tggctcccca
     2761 gaaaataaat ttcgtattct gccctggcag ccatatacga aagaaatcat agccgctgac
     2821 agaaaaaagg ccgatcacca ttgcctcaaa aaagccggta cagcgcatgg cttttttcag
     2881 aagttctgtc agctgggaca tccttttttc cgcatacagc attaaaagcc ttggtttaaa
     2941 ggaagaactg actttcaaaa taacgctgtt gcttaaaaac gcaatggttt ttacaactga
     3001 gatctgacct aagaccactg catccagcat ccggttggtg atccacaggt ccagaccatt
     3061 atttaacaca tttcccaggt tgttgatgga tgtccatatg cctgtagaaa cgatctccca
     3121 gatagccccg ggagaccatt tatcccgccc aaagcgcaga tccggcagaa gtttccggct
     3181 gacagctaca tttgccaaca gtaaaaacag cgccgctgac atagatgcca gtcccacata
     3241 ccatacgtgc gggaaccagt tcaggcatac aaaaagcagg atccctgcct gcaaaagata
     3301 ggctccgctt tgcagccgtt ctgttatatc gatgcggtcc gcgataaagg cggctgtttc
     3361 aaaggcagtc cgcatgacca ccatgatata gcgcagaagg actaaaaaga acaatctttt
     3421 tacatccggc accagttcct gtgggatctt acaaaaccag gtgatcttta agatcatggc
     3481 gatccccagc aaaaataccg ttcccccgag aacggcatct gccacaatgg tagaggaaaa
     3541 ataccggttt gcctcctctt tctgtccttt gtggtatgcc acggaaataa aacggcctgc
     3601 gtaggcgttc aggctaacgg agatcacatc cacatagctg ataaaggtat tggcaagggc
     3661 tacaaagcca taggcttcca ctccgatatt attggtcaca taaggcgtga tgagaaaatt
     3721 catcagggtg ttgatcaaaa gggaaaacag cgcccagaac atattcatca taatggcccg
     3781 cttcatactt gtttcctgtt tctgattgtt catcttttcc tgtacgcctc ccacagaatt
     3841 tttgcggaga tcttccagct gtacttttcc tctgtgagtt tttctatttc tttctgatcg
     3901 tccagatact cttccctgga ccagtccgca gcctgtaaaa tagcctcttt cagcttttca
     3961 ggatcctcac cgtctgttaa gatacctgtc tttccatttt gtgtttcttc ccggaaagca
     4021 ggaatatcac tggtaatcac cggtttccca taactgtaag ccattaaaag ggcgccgctt
     4081 ccgtaaatgt cccggtacgg gaacaggcag atatccgcct gacggtaaag ggcatccagt
     4141 tcttcatcgg gaatgtgctt ctttaaaagg tccactgtat cttccagccc cagatcccgg
     4201 atcattgccc cataatccgt ggggtccagt ttgggatgct gggctccggc aatgatcacc
     4261 tggatctttt cccgtttttc agccgggatc tgtgccaacg cttttagcag gatatccact
     4321 cccttatatg gccttaaaat gccaaactgc aaaaaccgta tcttccggtc tgtaagccca
     4381 aaatctgtat tttcctcttt tctgatctgg gtatagcttc catgaggggt aatacagatc
     4441 ttttcctccg gaatttgata ctctttcatc aaaagctgcc tgcaatattc attatgcacc
     4501 accaacaggt cacagcagtt ataaaaacgg ctgtacagct ccctgtcccc cgctgaggct
     4561 tcgtggggaa ggagattgtg tactgtatgc accagacggg gaatgtattt tttattttta
     4621 cagtacaggg ggatctcgta ttttgcgtct ttaaaggact gtacgtggac tacatcatag
     4681 ctgccctttt tgatctcttt ttgcagacgg aacaggcctt ttccatattc ccatacagca
     4741 gccatttttc cttttccgcc tgcgtacagc ttatctttcc agatgacccc atccagatcc
     4801 gttcctgcgt tctttttaca aaatatggtt attttcgcat attttctcaa ttccctgcac
     4861 aattctacca catacacact gtttggcagg aactggtcta ccattaaaac tttcattttc
     4921 tcatccccct gccgatccaa aaggtcccat atcccatggc agttcccagg gtattggtca
     4981 gaatatcatt tacatcacag gttccggagg ctgtgatcat ctgtatggtc tcgatccata
     5041 ccgaaagcat gagaccgttc atcactgcat atcccggatg gtcaaaggca ggatggatca
     5101 gcggaaacaa cagtcccacc ggcacaaaca gggctacatt ctgcagcata tgccatacag
     5161 gctgtaaact gtgtttttgc agcgcttccc ctacgcctgt aaaaagctgc atctgcaatc
     5221 tgctgtctgt tccccctttt ctccataaaa gagtcacagc cagtactgca agcacatagg
     5281 tgagaagcag ggcgcagccg cctttattta aggtccttat attctgcatg ataaaccaca
     5341 gttctccgcc gaagatcaaa agagacaaca gcacaagaag cataaaaagc acaatgctgc
     5401 tttctccagt gacctggaac acaaaaaaca gcataacgcc tacacagcca aaaatacata
     5461 aaaggatcat ggctgcaggc actatcccgc tcctgttctg gatccgtccg gagatcacgc
     5521 ctaaggcagc gcaataagcc ccgaacagga tcacagcgca gagaaataac tgcatcccat
     5581 cccgcatatc caaactactt aacatattgt ctcctctttg gccctaccct ttgtttattg
     5641 caggcagatc ccattgcgat aaataaaaac aaagtctgat attcataagt ggacattgaa
     5701 cgttctgtcc agccaaatac cataaaccac aggatatacg ccgcactgaa cagataaccg
     5761 gtccattcca tccgctctgc tcctatggtg gaaatgatca tagccgtaaa atacgcggca
     5821 actccaaaca gacctacatt catccacaat tccagcaaaa cattgtgggc gcctgttgtc
     5881 atgcttccca taaaagaatt tcgtgaaaat gcctgatgga ccatggcaac tgcctgtcgg
     5941 tccctccaga acattccata tccatatccg gtaaatgtat gccggttgat cataacctgg
     6001 atgatctgcc gccacaacgg gatacgacct gttaaagtgg cgtcctttcc gatcaaggaa
     6061 aataatggtt gaaacagcgg taaaatcgta agagccgtta tgagaaaggc aacgcttaag
     6121 gtaacataga tcaccccaag gggcagtctt tttcccggtt cctccttata ttttccaaac
     6181 agcacataca gtagcggaaa gatcaggcat aacacagctc ccttggcatt gcacaggacc
     6241 agaaagatcc cctgtgcaag taaagaaagg agatactttc gcgatacgat catctgtttt
     6301 gcccgcagaa tacgaaacca tgcgatctga aggagaatgc aaatggaaaa ctgggatgcc
     6361 gcattgttct ttgtccttaa aaaaccggta aaatcatgtt ctccctgttc ctgtgagaaa
     6421 acagctcccg gtcttaaaag cataaaagca aatgtaagac aggtaaatat cacctgtgcc
     6481 gctgcgatca gatgaaacag tttctccccg tcataatact ctaccatcca aagtgcgaaa
     6541 aagatggtgg ctgtataacg gatacaggaa ataagctgtt cactgggata ccgtgtcacc
     6601 agcatggagt ctaagaaata gacagctgcc atgaaataaa taaccgcgta tttttctttc
     6661 agatccagca gtttcaggct cataaagccg tcttcactgc tggaaaacag catgagaaga
     6721 cgctgcgccc caaaggatgc gtattccagg atcttgctgc tggcagaacc aaggagcatg
     6781 gtatatttac ctggaaatcc aaagctgaca agcaggataa agataacagt tgtgtcaacg
     6841 atccggctct ttaaaagatc tttacggctg ctctgactgc tcagggtctg tctctgactg
     6901 ttctgtatcc tcattttcaa gttcctcctg agactcttcc cttccgctct cattcattcc
     6961 catctgtacc agagcttcga tggcctcctg gttggaaagc cggtttgatg tctctaacag
     7021 atcatctgtc agttcttcta acagaccatt aaatgcggta tcttcctctg tatgcggatg
     7081 ggtagatttt aaagcctggg tctcttttgc ggatttcatc atgtcttcca gatcattcag
     7141 atcatccatg gtttctttca ttgtttctgc ttccggcttc ttcggttttc ttccccggtt
     7201 ttctactaca ttccgttcac cgccgaaata ttttccccct gttttctggg cggcatttac
     7261 aacacagcca aggatacgga tcccggactt gtccagcttg tcaatggcat tctggatcat
     7321 cggaagagcc acatcatcat aacggatcac aaaaagcact gcctgggcga tctggttcag
     7381 gcttaataca tcagaaacct gtccaacaga aggagtatca atgatcacat agtcatagtc
     7441 cttttcaagg cctctgataa aatcaaatac ctgtccgtcc ataggaatgg catttcgttc
     7501 cagtacggat ggaagcaggt ccagataccc ggtaagggag atcactgctt ctagcggatc
     7561 cgtctcccct ttataaaggg catttaatgt acggctgtaa tccacagaat gtaaaaacat
     7621 actgcccaga cggggatttc tggtattcag atccacaaga aggaccttct tttccatatc
     7681 ggacagctgt actgccagat tggcagcaac tgtactcttt ccttcccctt cttctgcgga
     7741 tgttacataa aaactgcagg tctcatcttt cagacccaga cggttctgca aaatataggc
     7801 agcagatgta aaattctggc ggaccgctga gtcggtcatt ccatcttttt ccaaaatcac
     7861 ccttccgtcc ctgaaatatt cctggtcatt tgggatgatc cccatagttt ccaggccaaa
     7921 cacattttcc acatctttca ggttcagcag agtcggacgc atgatcagct ccagcattgc
     7981 cataccggct cccatgataa agcccatcac acctgtgatc agccagatat gggaaaggcc
     8041 gcccattccc atgatatatt ctgcctgcgg ctcatcaata gtagaaacag atcctaccat
     8101 taatgttttt ggaagcacct cacgggaagt atccaagatc gcgttcatta agcggatccc
     8161 tgtgtctgcg tcatgccatt ttaagctgat ctcaatgatc tgtgtctcat tgtaacgctc
     8221 tatatccaga tttctggcga tggccaccgg atccggatca acgattctca cactttccgc
     8281 tgcctcacgc agaacccgct cgcttttcat cacatacccc actgcatcta ccatatcctg
     8341 agccagataa aaatcattag ggttaaaata actggaattc cctgtaaaat ttcctgtctc
     8401 agactgggat gttactgcaa tggaacagtt gatcctgtaa tttgtataat ttccctgtac
     8461 atacgatatg ccgcttaagg cagcaccaaa cccgcatcca accaggacaa acgccaggat
     8521 catcttccag cgtttcccga tcgcgtaaag taaatctcct actctgatct gtacaccatt
     8581 catcgttttt tctcactgtc ttttttctta tcttcttttt ttcttctgcc tgccagtgta
     8641 atgcctgcaa agagcagaag aatgatcaca acggttccgc caaccatacc ggctgccagt
     8701 gtaagaatgc cccgaagagt cagtactgtt acattccagt tctgtacctg cgctgtatct
     8761 gctactgtaa agtcataacg cttctccccg tcatctccct gaagcacata gtaactgcct
     8821 tcttttaagt tggtgaacat ttttccgctt ttccctgata cccaggactc gatcagctga
     8881 tcctgactgt cataaaggga taactggata tctgctacat cttttcgcag gatcatatcc
     8941 gttccatgga cagatgtacg aagcatcctg tttgtcagat ccatttctgc ctggttttcc
     9001 ccgtttatgc gtccataggc atctacatct tctgtatatt cgatcggatc cagctggtaa
     9061 tcttctatgg tctgctgctg tctgatctta taactggtat tcttatccag gtctgtaagg
     9121 aggatctggc cattttggtc tgtctctaca gtctgggaaa cacctgttgt tccattggta
     9181 actgtaaata ctgccccgga cagcggctgc tgctgatata attcatctga aagttttaaa
     9241 gtgatctttg tcttttctgt atcgatctca tggatcgggg ggtcttcccc gtttttcttc
     9301 tctaccagag cttccggctg ttcgttgatc ccggcgtaat agcggggaat tcccgcctgt
     9361 tccagtgtat agcttcccag atacagttcc ttactctcac cgtaaccatt ttcatcacag
     9421 gtaatggtat ctaccagctg tcccgctata taacgcacgg taccatccag cgtggccaca
     9481 tcttctgctg cccgtacctg gaaggttccg cctcctaccg ggtctttgct taacagatct
     9541 gacatctgca cccggatcac gttcttgctt ggtgatacac ggatctggac tacaaaagga
     9601 tccgtccagt catacatctt atccaggtta aaataggtat cccctgccgc gtcatatcct
     9661 tccggttcat ttaattcccg gaagaaataa tctccctggt agattttttc cggcagataa
     9721 aatacgcctt cctctgttgt ctcatattca cgataggaga tcttttccgg ataataggta
     9781 ttcaggatct gaagggctcc cattccattt tgcagtcccc agtctgtgcg gtacagttca
     9841 aagcggactc cctcatccgg gataatacgg tctgtttcgt catccaccag ctcggtaaat
     9901 accgctgatt tgatgccttc gtcaccgtaa ccgcctccgc cggtctggat gataggctgc
     9961 tcctgtccgg atccgcctcc gtctccggcc ccatctccta catcctcatg atcgccgttg
    10021 cctttatatt ccttccagct ccagccgtca tcatccggac gggtctcctg tgccgttgtc
    10081 tcctctacgc cttctgtttc atcatattta tatcctgtat agtcacttaa aagatacagg
    10141 cccaacagcg ctacgatcag catcaggaca aatacaagtc ctgtcatcat atccgtagca
    10201 ggctgccagt aattatactc tccgttctgg tctttcctac gccgtctcat gcatcctgtc
    10261 ctacctttag gattttttca aactcttcga ttgataatgg tctggaaaat acatatccct
    10321 ggccttctgt acatccgcac ttacgcagaa gtgataactg ttccatgctt tcgatcccct
    10381 ctgcggtaag ggtcagcctc agtgtctttg cccggtcaaa cacatcctgt acactgccgc
    10441 tcttcttctc tttattcagg ctgcgcagat ccagtttcag tacatccgct ccgatctctc
    10501 ctacagagtg cagtcccata tagtcaccgt caaaaccatc gagggcaatg cggaagcctt
    10561 tctgctgaag ggacttttcc acttcggata caatgccgta aacctctttg tacgcatttt
    10621 ccgcaatatc gatatttaaa tattttggcg gtatcctgta ttttttcaga agttcatcaa
    10681 agatctccac aacatccatt gcaagcaggt ctgtttttgt tacgttaatg gaaatcgcaa
    10741 gaggttttat accctcatcg atccatctgc ggatcattgc gcatacatct tcccagatct
    10801 tctgatccag ttttgtaata tatccgttat tttccagtac cggaataaat actgcaggtg
    10861 agatctcgcc caacgccggg tggttccagc gcaccaaagc ttctgcacct atgatctttc
    10921 gtgtatttaa ttcatactga ggctgtaaca gaaaaataaa ttcattcttt ttaaggccat
    10981 ttaagatctg cggcatcaga agacgctcat ttccaatggc tgagtctgca gttgccccca
    11041 taccggaagc tgaataatca tctttctttg gcagacggtc caacagatcc atgatctgtc
    11101 ttgtctctct gcgggaacgg tagcgttcct gttcctgagt agtaggaagg accactctgt
    11161 gaaaaacttc cagaaagatc cccatttcac gatccattgt attacggagg atattattag
    11221 atatattaaa aagaatggac aggataacac ctgctatgga tgtatagaat gcggtatcaa
    11281 taccggtcag aatggactgc acactgctaa gtgccgcctc aaccgtaacg aagttaatat
    11341 ttctaagacc cctaagaagg ccgataaagg tacctaaaat tccaagacct gttaatgttc
    11401 cagggatctg tgcaacgact ccctgccagc tgtaaagagc caggacatct tcatttaaaa
    11461 tatcttcaag atcactgacg atctgtccat tctggcgctg ctgctttacc ttgtccctgt
    11521 attcccgaaa caactggtcc attttcttct gtctgaaaaa acggtccatc tgtaaagcgg
    11581 tgatccagac cgacttttcc tctgcttccg aggctctctg tatactcttc tggcctgtta
    11641 acagtccctg tgtaaatgta agtaccggta tcagtccata tataattcct aaaagcacta
    11701 caacgcccat aaccgcaact acgatcatag aaagcatgtt aggcgcataa gctgtcaaaa
    11761 tgccacagcc tgacagcaat ataacaataa atattggcat caatctcttc atactgtgcc
    11821 ccctcttttg tttttaatga tttttgccta ttctactgaa ttttaccgca tatttcaagt
    11881 cttttaccct gtattttttt tgacacaaaa atctttaagg tattataata taataaagaa
    11941 aacaatatat tttaggcaaa gcagtcgaaa gactgtgacg caaagctata gggcctttca
    12001 aatggcagcc agttgctatt ttatttcttt acattccgtt cacaatttac ttacaatttg
    12061 aacaaacttt cttcacgatt gttttctata ctaaagagcg taaagagtta atgcaaacaa
    12121 ctttttttca taatactcgg cggactggaa agttttcagt ccgcctcctc ctttttaggg
    12181 atgtactctt ggaagctttg tccaccggta taccggaaat gtgtgttttg cacaaaacag
    12241 cttttcatat ttttcaatct ttgtgcacac ttttccaaac tcctatgcta ttataataga
    12301 cgtaaccccc caatacatta tatagttttt gctacacccc cataagaaac gaaatacctt
    12361 ctccttaaaa agacccgttc cccgacgggt ctttttactt acctttttac cctccaaact
    12421 atcccccaaa aaaattttaa attttttcaa aaaaatgttt gacaaaccca taccgctgtg
    12481 ctattatata cgagtcagcg gcgaacagtc aagacaaaac agaataaggc ctgatggtca
    12541 agcggtcaag acgacgccct ctcacggcgt aaacccgggt tcgattcccg gtcaggtcac
    12601 taaaaagcaa gtggaaatcc acttgctttt ttatatttaa aattgacatt agtgattaaa
    12661 tgtgtttaaa tatcccccat ggagctattg cgaatatggt ctttgcaata tctaatatcc
    12721 ccattttata aatatagata taaaaatagc aggggccgct gaaaggaggt caggctttca
    12781 gcggcccctg cggaccttta gcgacttttt accttaatat aaattcttca ccttaaaatc
    12841 cttctccaac tctctcctct ggtccttctt agcaatatcc gctctcttat catacaactt
    12901 cttacctctt gcaagtccca cctgcacctt caccaggctt cccttaaaat aaacctgcag
    12961 cggcatcagc gtatatccct tttccgcgat ctttccattt aacttcatga tctcgtattt
    13021 atgaagaagc agctttctca ctctcaaagg atccttatta aaaatatttc ccttctcata
    13081 aggactgatg tgcataccat acacatacac ttccccctta tcgatcttca caaaagcctc
    13141 tttaatactg cactttccca tacggatgga cttaacttcc gtaccaaaca gctcaatacc
    13201 agcttcccat ttatcatcaa taaaataatc atgatacgct ttcttattat tggcgatcag
    13261 cttaatactt tccttagaaa tgataaacac ctcccatttt cagtttcttt gccctgcaaa
    13321 cagaactgat ctctgttttt ctatctcatt tgctcccact atcataccat tatactgcct
    13381 gattttccag aacaaaatcc acagtccgca aaagcttgtc cgtaccggct acaactaccc
    13441 gtactgtctg gcccagctta tacacttttc tgctcatctc gcccaccagc tcatatctgc
    13501 tctcatcaaa aatataataa tcatcccgca gctcactgat gcgcaccaga ccttccactg
    13561 tattaggcag ctccacataa aagccccagt tagtaactcc ggagatcact ccctcaaagg
    13621 tctgtccaac aaacctggac atatattcgc acttcttcat cttctcagtc tcgcgctccg
    13681 cctcatcagc ccggcgctct aatgcagaag tctgtacagc cacctgcggc agcagtttct
    13741 cataatgggc aactctcttc tctttcaatc ccccacgcag gcactccttg atgatcctgt
    13801 ggatctgcag atccggatat cgacgtatag gtgatgtaaa atgggtataa taccttgcag
    13861 ccaatccaaa atgtcctgta cactgggtgg tatactttgc ctgtttcata gaccgcagga
    13921 ccagtcttgc aagaagaggt tcctccagct tgccttcgat cttgtccaat agcttctgca
    13981 gctcctttgg atgcacttcc ccattagtca atctcagcga atatccaaaa ttattaataa
    14041 acacacctaa ctgcttcatc ttctccggat ccggatcatc atgggtcctg taaaggaaag
    14101 gaacagactg ccagaaataa tcctctgcca ctgtctcatt ggcaaggagc ataaaatctt
    14161 caatgatctt tgtagctaca ttccgttcat aaggatggat atccaccggt ttccccttct
    14221 catccagaat gatcttactt tccggaaaat caaagtcgat ggccccccgt ttgctgcgct
    14281 tctcccttaa aatagcagac agttccttca tgcgctcaaa catagccaca aaatccttat
    14341 actcttccag taaaccagcc ttttgctcct ctttctcctt tgtatctgcc tccgacaaaa
    14401 tagcattcac agccgtataa gtcatccgcc tgtcagaccg gatcacactt tcgcagatct
    14461 catgatccac taccgttcca tgaggatcca cttccataag gcagctcatt gccagacggt
    14521 ctgttccctg atttaaagaa cagatcccgt tagaaagctt gtgaggcagc atagggatca
    14581 cacggtctac cagatacacg ctggtgcttc gctttaacgc ctctttatcc agttcactgc
    14641 cttcttttac ataatgactt acatctgcaa tgtgaacgcc cagacggtaa tttccgtttt
    14701 ccagcagctc caatgtcacc gcatcatcca gatccttggc atcttccccg tcaatggtca
    14761 cagtctgcag atcccttaaa tcctttcttc cctcaaaacc ttccggaagg tcctttaagc
    14821 cctctgtagg caggctgtcc ggcacatgtt ccagttcctt cattacttcc tcaggaaaga
    14881 cttctggaag attgtaagca cgtaccaggg ataaaatatc cgtaccagga tcatttacat
    14941 gtcccaagat ctccttgatc cgtccttccg gcttcttacg ctcatcaccg aaatctgtta
    15001 cctttactac taccttgtga ccggtcacag ctcccatatc acagccctgt gggataaaaa
    15061 tatccttagc gatcttctgg tcatcaggga tcacaaagcc aaaattctta ttcttctgat
    15121 aatagcctac cagctggctg ttggcatgtt ccaatacctt taaaaccgta ccttctgccc
    15181 tgcgtccgtt gcggccctca gactcaatga cgatctgtac cttatcacca tttaaagcac
    15241 ttcctgtctt atcctcaggg ataaatacgt cctgctccat tccttctact gtaacaaagc
    15301 caaagcctct tgcatgtcct gtaaaagtac caactacagc aaatgtctcc ggttttccat
    15361 attttccctt ttttgagatc ccaagctttc cttctgaaac cagttcgtcc agcacttcct
    15421 gtaattctcc ccgcattgcc ttggggatat ttaaaagcat ggcgatctct tttgctttca
    15481 taggcacata agccttgtca tgtaccagtt ctgtcagcat ctgttttctc tgttctaata
    15541 actctctgtc catataacct cttttcttta ttactttatc tatgtctcac ctaccatcca
    15601 ggcaggcgat ctgtgccctt atatttgcct ttgtattaaa taagaatgtg ccttggcaca
    15661 ttctcgcgcc gagcgcggtc gcttgcgaca aacttcctca tcgcgcgagt gcgcatcgtt
    15721 ggcaggtcgt gcctttttgt gtactaggga gttccaacgg aatttcctag tacataaaaa
    15781 aacacccttc tgtctgttac acgaagggtg ttttgactgt ctctttctat tatccctgag
    15841 tcttcataag aatattcagg accagtgtaa ggatcataaa tacgatcgca ccgtatctgg
    15901 taaaatgctc cagagctcct tccatagaac gtcctttatt cttgctccag tagctgtctg
    15961 ccataccacc gatggcactt cccagtccgt tggacttgcc ttcctgcatc aggatgacta
    16021 cggaaatagc tatacccaga acaacatata taatggataa aagaacctta actactgcca
    16081 tttgtctaca cctctccgtt tctaatagtt aaacaaaact attttatact atatcataac
    16141 ccatttgaaa ttacaaggct ttttgtcacc gttttctgcg tcaaaagcaa actcaacgat
    16201 tcctgttgca tagcagatac aggtgaatac agcaaagagc actctcatct gcctttctat
    16261 ttacagcttt gctccatctg ccccaacaga tgcgccgtct ggtgttactg tatttaccag
    16321 catggcaccg gactcatcac agaaataatc cttttcattc cagtggatcc atcctgtttt
    16381 catataacct tcttcatcaa agaaatacca taaagagtcg atctgctgcc agttgtttac
    16441 aggataagag ccatctctgt ttacatacca ccagcctttt tcattattct cccagtggcc
    16501 tttccagtct ttcatctgct cttttgtcac ttcaaaccag gctgactctt cccagtctcc
    16561 ctgattaccc ctggagtcta ccgcacggac cttaaagtaa taagtgccct ctttcgggat
    16621 cttctctgaa aagttaaagc tgttctcctt tacagtataa ata
//
LOCUS       DBMDUA010000046        16627 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_1989_length_16627_cov_3.540542, whole genome shotgun sequence.
ACCESSION   DBMDUA010000046 DBMDUA010000000
VERSION     DBMDUA010000046.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 16627)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16627)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16627
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1989_length_16627_cov_3.540542"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="ACZPCL_05370"
     CDS             <1..140
                     /locus_tag="ACZPCL_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1161941.1"
                     /translation="DKRGTGLGLSIVKEIIQAHGENIDVISTEGVGTEFIFSLPSSTS
                     L"
     gene            348..1382
                     /locus_tag="ACZPCL_05375"
     CDS             348..1382
                     /locus_tag="ACZPCL_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161942.1"
                     /translation="MIRLKKVTAVLLSALLVSSCMAPCTFAASKTKVGKINLTIDTDI
                     RSGSSGGEVEVTPTGDNTEYFYIDSVEVTNDEGDDWSKSNPPEAEIRIGLEDEDEYTF
                     SGSSSSNFKLTLASSIKSRYDKVEYVSARKTDGGATIILNIRLVFDKDADMSNAAAPG
                     SVEWSASSEGTATWGDVSSAKYFQVQLYKDGNLVTPPDGSASTVSVYGTSYDFSSWVS
                     AGSKYTFKVRSVKASNNAKSKWVSSSSWTPGSNTSDNNNTNTFPEGWQKAADGIRWWW
                     QNPDGSYPASQWKETDGKWYYFDAEGYMATGWINVGGVDYCLAPDGALYVSCTTPDGY
                     NVDANGAWIH"
     gene            complement(1526..2470)
                     /locus_tag="ACZPCL_05380"
     CDS             complement(1526..2470)
                     /locus_tag="ACZPCL_05380"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HIR1161943.1"
                     /translation="MGMKCIGVDIGGTSVKIGLFETTGELLDKWEVKTRKEDGGVNIL
                     PDVAASIRKKLDEKGLDLKKDVAGAGMGVPGPVMPDGYVEVCVNLGWRDLNPQEELSR
                     LLDGIPVKSGNDANVAALGEMWQGGGKGYSDLVMVTLGTGVGGGVILDQKIIAGKHGL
                     GGEIGHIHVRDDEWEHCNCGGVGCLEQVSSATGIAREARRTMAKSDKPSALREFGDNV
                     SAKDVLDAAKAGDEMALEVMKTVSHYLGLALAQVALTVDPEIFVIGGGVSKAGQFLID
                     MIQKDYDHFTPISENKAKLGLATLGNDAGIYGAARLVL"
     gene            complement(2575..3858)
                     /locus_tag="ACZPCL_05385"
     CDS             complement(2575..3858)
                     /locus_tag="ACZPCL_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HIR1161944.1"
                     /translation="MDNSSLMNRENKKRQLRRQIVPPRYQTEEESEQAIRNAHKKTVR
                     KRLAVLAVVAVLLGGAAFGIYYYDRYHSFYDYQVVWEKSLAEENQEAAVKGEGSFCEY
                     VDFGDGVIKYTKDGASYIDGRGKTIWIQSYEMKSPFVSVNGEFAAIGDKQGNSIYICD
                     RSGCQGQATTALPILSLSVSAKGVVASVQEDSKASYIYLYKKDGSALDIYVKSLLSGD
                     GYPVDVSLSPGGTQWITSFMYLDQGMIKNKIVFYNFGLGKNDPKRVVGVFLPEDLSDA
                     MAGRVRFMDESHAVIFTDKGLQFFSTRVETSPESVNQILLDENVRSISYTDKYAGVIT
                     DNGEGADPYCLRVYRPDGSQVFETTFSYQYTGFDIDGDLVTLYNDNSCCVYNMAGTEK
                     FSGSFDFTVTKVLSGRFPGTLLVLGTQKMEEIRLK"
     gene            complement(3972..5150)
                     /locus_tag="ACZPCL_05390"
     CDS             complement(3972..5150)
                     /locus_tag="ACZPCL_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR1161945.1"
                     /translation="MGELYELYPKLPKNIRQIGERDTVLRLYVEDYVNTYLKRLKPTA
                     GQNLRVGLLLGNREIHEDMPYVFVDGAMEMEILSQEGEKVVFTEEAWKKAYQQVETMF
                     PRRTVQGWFLCAGEGVTLSPLNYWRQHSQYFTGKNQLMYLNSGLDGEEAIYITSSDGF
                     YQLRGYSIYYERNQMMQDYMIARKDILREESKTDDRAIRDFRKKMDEHKTAAVRQRSA
                     VSVLSGLCSVLAVMVLAGGVAMFNNYKRLHEMEQVVASVDPEAVIREARGNLSSVAGN
                     RTTGETPVEVIPETMAPGGSTAGTVEASLTKETPDQTVPPVQTTSEPAQTTAAQTTQA
                     QAANAEASYKVYVVGEGETLYGICFKEYHNIRKLQEICQINSLTDENSIYAGQKLLLP
                     "
     gene            complement(5227..5826)
                     /gene="yyaC"
                     /locus_tag="ACZPCL_05395"
     CDS             complement(5227..5826)
                     /gene="yyaC"
                     /locus_tag="ACZPCL_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HIR1161946.1"
                     /translation="MRLWKSFRVQRRDEISYYDTMGEFEAERFAVNLNQLIAEAMAEK
                     QKAGVIFLCIGTDRSTGDSLGPLVGHKLRKCRLKKAAVIGTLDKPVHAMNLEVYAAYI
                     RTHFPDHVIVAIDASVGSPDHVGFATLGKGALQPGLGVSKELMEVGDISITGIVGGPG
                     SHDPVMLQSVRLSMVMKMADCICESIALVERFWENTAII"
     gene            complement(6020..6940)
                     /locus_tag="ACZPCL_05400"
     CDS             complement(6020..6940)
                     /locus_tag="ACZPCL_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR1161947.1"
                     /translation="MKETALVIMAAGIGSRFGGGIKQLAPVGPSGEIIMDYSIHDAIE
                     AGFNKVVFIIRKDLEKDFKEIIGNRIEKQIPVIYAYQELNALPEGYEVTEGRTKPWGT
                     GQAVLMVKGLIDCPFLVINADDYYGKEGFRKIHEYMVNEMTEDGDVYDICMGGFILKN
                     TLSDNGAVTRGVCEEENGILKKVTETYDIRMMEDGLHAADEAGNPVKVQPEQLVSMNM
                     WGLPVSFLNELEKGFPEFLDSLKPGDIKAEYLLPKIIDKLVHEGKAKVRVLDTPDKWF
                     GVTYKEDKQAVVDAIKGLIDAGVYKEKLFD"
     gene            complement(7233..8435)
                     /locus_tag="ACZPCL_05405"
     CDS             complement(7233..8435)
                     /locus_tag="ACZPCL_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR1161948.1"
                     /translation="MEREEQEAWELAKQLVQIDSSDPGAYEEEIGLWITDWFKKQITD
                     FGGKLADQIHVSRLEVLPGRYNVMAKIPGKTEGPGLIYICHMDTVMLGDGWDEDISPL
                     GAMVRDGKMYGRGACDMKSGLACALTAFKNMVKKVGERGELPEKPFSMICSVDEEDFM
                     RGVEAAIKAGWVTKDDWILDTEPTDGQIQVAHKGRTWFELTITGITAHASNPWKGADA
                     IAAMAEAISSIRKEIKACPKHHDLGISTVTFGQITGGYRPYVVPDSAKVWIDMRLVPP
                     TDTAAAKAIVDRAIRKAEEEIPGVKGSYIITGDRPYVEKDDHSKLLANLKAAVKQTTG
                     EEAVVGSFNGYTDTAVIAGTLMNHNCMSYGPGSLELAHKPNEFVPYEDVCRVQKVLTK
                     LAENVLFG"
     gene            8636..9499
                     /locus_tag="ACZPCL_05410"
     CDS             8636..9499
                     /locus_tag="ACZPCL_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1161949.1"
                     /translation="MKEIKSPFWRTIYEYGLITVSIWIMVVGIYFFKFPNHFAFGGIT
                     GFSTVVSQLTHWSASDFTSVTNTALLLLGFLFLGKGFGLKTVYATMVMSVSLSLLERF
                     CPLSAPLTSQPLLELMFAIFLPAVGTAILFNIGASSGGTDIIAMILKKYSSMNIGTVL
                     MLVDIASVVASYFIFGVETGLFSTIGLAAKSLVIDDVIENINLCKCFNIICDDPKPIC
                     DFIINGLNRSATVYHAEGAFTHHDKTVIMTTMKRSQALKLRNYIKRVEPTAFMLISNS
                     SEIIGKGFLAN"
     gene            complement(9496..10632)
                     /locus_tag="ACZPCL_05415"
     CDS             complement(9496..10632)
                     /locus_tag="ACZPCL_05415"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1161950.1"
                     /translation="MKYRTMRFIGGEENVSLLGLGCMRFPLNEDGTIDEAQAEAMIGR
                     ALNAGINYIDTAYPYHKGESEPFLGRALKKYPRNSFYLATKLPVWLVNSVEDAERLFK
                     EQLSRLQTKYIDYYLLHALDAGRWEKLVKEGIIEWAEAKKASGKIRHLGFSFHDTYPV
                     FREILTYRQWDFCQIQYNYIDTQVQAGDKGYELAAKMGVPMVIMEPVKGGRLANLPEE
                     AKEALNEADPDRSAASWALRWVGSHENVKVILSGMSSMEQLEDNLETFGKFQPLTEAE
                     EEAVEKAADLIRSRVKNGCTSCRYCMPCPFGVNIPEMFSIWNNCAMFDEEEKAKKAYA
                     EQKVQDASADLCKKCGACEKACPQGLSIRADLEKVAKELNTEEA"
     gene            10869..12260
                     /gene="rpoN"
                     /locus_tag="ACZPCL_05420"
     CDS             10869..12260
                     /gene="rpoN"
                     /locus_tag="ACZPCL_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR1161951.1"
                     /translation="MDLKLQVKQSQVLSQRMIQSAEILQMTSQELNTYINDLALENPV
                     IDIVEPAAGDSQKESIEQQQWLNSFNEENYYLYQRQNNDDDYDFKDTWNINTDEGETL
                     ADYLWSQLISEDFSEQETEIIHFCLDSLDNRGYLTETQEDIAAYFKTDTETIDRILEE
                     LKSLDPAGVCAFNLEECLKLQLERKKMLTPVMESLIDNHLDMVAKNQIPAIARALHLS
                     SGEAAGYCQIIKSLNPKPGVSFSSRDQLRYIIPDVTIVKFKDHFDILLNESMYPGIEV
                     NSYYRQMNQAPDSQELKEYLGNKIRQAEWVKQCITQRSKTMMDVSRVILERQEAFFSH
                     GPNYLKPLRMTDIAEILGIHESTVSRAVSKKYLQCSWGVYPMNFFFSRNVGTSTSSTE
                     KSDDPSKAVTARKIKQLLHEIIAEENKKKPYSDRLLGEKLTEKGISISRRTVAKYREE
                     EGIPDASGRKEYT"
     gene            12373..12445
                     /locus_tag="ACZPCL_05425"
     tRNA            12373..12445
                     /locus_tag="ACZPCL_05425"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12406..12408,aa:Phe,seq:gaa)
     gene            12450..12520
                     /locus_tag="ACZPCL_05430"
     tRNA            12450..12520
                     /locus_tag="ACZPCL_05430"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12482..12484,aa:Gly,seq:tcc)
     gene            12546..12617
                     /locus_tag="ACZPCL_05435"
     tRNA            12546..12617
                     /locus_tag="ACZPCL_05435"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12578..12580,aa:Gly,seq:gcc)
     gene            complement(12735..13217)
                     /locus_tag="ACZPCL_05440"
     CDS             complement(12735..13217)
                     /locus_tag="ACZPCL_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HIR1161952.1"
                     /translation="MPQNKRESLIYSVMMCFTMVLWMSMYNVTLHMGGFSTEVLREGW
                     LGFPIAVVFAFCMDWFVVSGPAKGVAFRFFVNRDSSVLKKVIVISTCMVVPMVIIMSF
                     YGGLEACVRSGQWNLLLIIWLTNIPKNFIMALPFQLIVAGPLVRTVFRSLFPEGKVLA
                     "
     gene            complement(13587..14306)
                     /locus_tag="ACZPCL_05445"
     CDS             complement(13587..14306)
                     /locus_tag="ACZPCL_05445"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR1161953.1"
                     /translation="MKQSPYQLLMGVVLLGTVAFLSWQAGGLAAAGMQYIQSENGPGE
                     QKQAVVVIDPGHGGTDPGKVGIDGQLEKDINLAVAKKLKTYLEASDVKVVLTRDVDTG
                     LYSSKDTHKKMADMKKRCQLIEEAKPDLVISIHQNSYHEEAIRGGQVFYYKTSVRGKK
                     LAESLQKRFDYVLGDSNKRQAKANDNYYLLLHVKEPIVIAECGFLSNREEAKKLETEE
                     YQDRLAWTLHMGIMEYLNQNQ"
     gene            complement(14393..15889)
                     /locus_tag="ACZPCL_05450"
     CDS             complement(14393..15889)
                     /locus_tag="ACZPCL_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1161954.1"
                     /translation="MKKKRLIMTAALLSAFCMSAAGCSLYPGNKETQPANASQAETET
                     KDETKQQPTGETEEETEKPTDAHPEDIEPFPHGEKETKAEPMKNGKRIVLMTDIHYLA
                     DSLTDKGEEFQYMVEHGDGKLTNYVWEITDAAFEQVEALSPDVIILSGDLTLNGEKES
                     HKELAKKLEQVEKDGIQVVVIPGNHDINNRNAASFDGRSRQMAEAVSANEFAEIYNDF
                     GYDEALSRDPASLSYTYDLGPDMRLLMLDSCQYSPTNKVGGMIKTETYDWIDDQLDEA
                     WDDGVILLPVAHHNLLDESKIYVEDCTIEHSEELVNRLEEENVPLFLSGHLHVQHYMR
                     NEEDRGIYEIVTSSLSTPPCQYGVLEYRDDESFSYHTQQVDMEKWARKHKSTDENLLN
                     FNTYAPAALRTIFYNQSYDAMKDSEEEETGDLFVKLTQRQKEQMSEVYAQLNAACYGG
                     KAYEVVKEATTTPAYKMWQEYCYPMVLFEYLEYIVDDAVKDYNYLEVN"
     gene            complement(15939..>16627)
                     /locus_tag="ACZPCL_05455"
     CDS             complement(15939..>16627)
                     /locus_tag="ACZPCL_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HIR1161955.1"
                     /translation="FSSTATRKKLIENLMKEADTYGFDGFNLDFESLKSSAGPHYVQF
                     IREMSVSCRQKGLVLSVDDYVPAVYSAFYNRKEQGIVADYVIVMGYDEHFAGGDAGSV
                     ASISYVENGITGTLKEVPKEKLINSVPFYTRVWTEKDGKTTSKAYGMTAAKKWVDENN
                     VELTWQDKVGQYYGEIENENGVQKIWMEEAKSLGLKKDLVNQYDLAGIACWKLGFETA
                     DIWTIMDEVK"
BASE COUNT         4210 a         4264 c         3212 g         4941 t
ORIGIN      
        1 aagataaacg tggtaccggt cttggtcttt ccattgtaaa agagatcatc caggcacatg
       61 gtgaaaatat cgatgtgatc agtacagaag gggtaggaac tgagtttatc ttctcccttc
      121 caagttctac ctctttataa aaatttttac acgaccggca tattttaact tttccttacg
      181 atttgctcca gagccccttt ttaaggggtt ctttgcttta aaaaggggtg gaactgtgtt
      241 atactttaat gagccaagta gaaaaacaag cggctgcgtt aacagacatt tgtttttccc
      301 aaaaactgct ctcaggcaat ttttttaaag aaaggacgat gaataatatg atccgtttaa
      361 aaaaagtaac tgccgttctt ctttccgccc ttcttgtttc ttcctgcatg gcaccctgca
      421 cctttgctgc aagcaaaaca aaagtgggaa agatcaatct gaccattgac acggacatcc
      481 gttccggaag cagcggcggc gaagtagaag taactcctac cggtgacaac acagaatatt
      541 tctacatcga ctccgtggaa gttaccaatg acgaaggaga tgactggagc aagtccaatc
      601 caccggaagc tgagatccgc attggtctgg aagatgaaga tgaatatacc ttctccggtt
      661 ccagttccag caactttaag cttacccttg caagctctat taaatcccgt tatgacaagg
      721 tagaatatgt aagcgcaaga aaaacagacg gcggcgctac catcatcctg aacatccgcc
      781 ttgtatttga taaagatgct gatatgagca atgcggctgc accaggcagt gtagaatgga
      841 gcgcttcctc tgaaggtact gcaacctggg gcgatgtttc ttctgccaaa tatttccagg
      901 tacagctgta taaagacggt aacctggtaa cacctccaga tggttccgct tccactgttt
      961 ccgtatatgg aaccagctat gatttctcca gctgggtaag cgcaggttcc aaatacacct
     1021 ttaaagtacg ttccgtaaaa gccagcaaca acgcaaagag caaatgggta tcttccagct
     1081 cctggacccc aggctccaac acttccgata acaataatac caacaccttc ccagaaggct
     1141 ggcagaaagc tgctgacggc atccgctggt ggtggcaaaa tccagatggt tcctaccctg
     1201 catcccagtg gaaggaaaca gatggaaaat ggtactattt cgatgcggaa ggctacatgg
     1261 ctaccggatg gatcaatgtt ggcggcgtag attactgcct ggctcctgac ggcgccctgt
     1321 atgtaagctg cactacacca gatggctaca atgtagacgc aaatggtgca tggatccact
     1381 aattcattgc tgcgtgcttt gcactcccgc aattcaatta catatgacca caaaaaaccc
     1441 gctgagtcga tcacatcagc gggtttttct ttatttcaaa ttttctttat ttcagttttt
     1501 atttattcca gattttaatt ttcttctaca gcaccagtct tgcagctcca taaatacctg
     1561 cgtcatttcc cagtgttgcc agtcctaatt ttgccttgtt ttcagagatt ggggtaaaat
     1621 gatcataatc cttctggatc atatcgatca ggaactgtcc tgcctttgaa acgccgcctc
     1681 cgataacgaa aatctcagga tcaacggtca gagctacctg tgccagtgca agtcccagat
     1741 aatggctgac tgtcttcata acttccagag ccatctcatc tcctgctttt gcagcgtcca
     1801 acacatcctt agcacttaca ttatccccaa attcacgaag ggcagaaggc ttgtcagatt
     1861 ttgccatggt ccttctggct tctcttgcaa ttccagttgc ggaacttacc tgctctaagc
     1921 agcctacacc gccgcagtta cagtgttccc actcatcatc ccgtacatgg atgtgaccga
     1981 tctcaccgcc aaggccatgt tttcctgcaa tgatcttctg atccaggatc acgccgccgc
     2041 caacaccggt tcccagtgtg accatgacca ggtcagaata gccttttccg cctccctgcc
     2101 acatttcgcc aagggcagct acattggcat catttccgct ctttacagga attccatcta
     2161 aaaggcggct taactcttcc tgtgggttta agtcgcgcca tcccagattg acacatactt
     2221 ccacgtatcc atctggcata acaggtcccg gaacgcccat tccggctcct gcaacatctt
     2281 tcttcagatc cagtcccttt tcatccagtt ttttccggat ggaagcagct acatccggaa
     2341 gaatgttcac accgccgtct tctttgcggg tctttacctc ccatttgtcc agtaattctc
     2401 ctgttgtctc aaaaagacca atctttacac tggttcctcc tatatcaaca ccgatacatt
     2461 tcatccccat ggtatatatc ctctctttct tttattttac cgtaaactta cgccccacgc
     2521 atttgcgctc tactgcctgc tgtcgcaggc tgttcgttcg ttaatggtct acttttactt
     2581 taaccggatc tcttccattt tctgtgttcc caacacaaga agagttccag ggaaacgtcc
     2641 ggacaatact tttgtcactg taaaatcaaa acttccgctg aatttttccg ttcccgccat
     2701 gttgtaaaca cagcaggaat tatcattata cagtgtgacc agatctccat ctatatcaaa
     2761 tcctgtatac tggtaactga acgtagtctc aaaaacctgg ctgccgtcag ggcggtagac
     2821 ccgaagacaa tatggatccg caccttcccc gttgtccgtt atcacacctg catacttgtc
     2881 agtatagctg atgctgcgca cattttcatc taacaggatc tgattgacag attccggaga
     2941 agtttccact ctggtggaga aaaactgcag tcccttgtct gtaaagatca ctgcatggct
     3001 ttcatccata aaacggactc tgccggccat agcatcgctc agatcttccg gaaggaaaac
     3061 tccaaccact cgcttaggat cattttttcc aagaccaaag ttataaaata cgatcttgtt
     3121 tttgatcatt ccctgatcca gatacataaa agaagtgatc cactgggtcc ctcccggtga
     3181 aaggcttaca tccaccggat atccatctcc tgaaagaagg gattttacat aaatatccag
     3241 agcgcttccg tcttttttat aaagataaat ataacttgct ttggagtctt cctgtacaga
     3301 ggctaccact ccttttgcag atacagataa ggacaaaata ggcagcgctg tggttgcctg
     3361 accctggcag ccgcttctgt cgcagatata aatggaattt ccctgtttgt ccccaatggc
     3421 agcaaattcg ccgttgacag aaacaaaggg ggatttcatt tcataactct ggatccagat
     3481 ggtctttccc ctgccatcaa tataagaagc tccgtctttg gtgtatttga tcaccccatc
     3541 accaaagtct acatactcgc agaaacttcc ctctcctttt actgctgctt cctgattttc
     3601 ctctgccagg ctcttctccc acacgacctg ataatcgtaa aaggaatgat aacggtcata
     3661 ataataaata ccaaaagcag cacctcctaa gagcactgcc acaactgcca gaacagccag
     3721 tcgcttcctg actgtctttt tatgagcatt gcggatcgcc tgttcacttt cctcttccgt
     3781 ctgatacctg ggaggaacga tctgacgccg caattgtctt tttttatttt cacggttcat
     3841 taaactggaa ttatccatcc ttgtctcctg tgaaatgttc ttttcttcat actcgcttaa
     3901 gtatacaaca tttcacagga aaaatcgaca gccaatcctt gcaaaacaaa aaatctccgt
     3961 cctgatatat cttaaggaag aagcagcttc tgacctgcat aaatactgtt ttcatctgtc
     4021 agggaattga tctggcagat ctcctgaagt tttctgatat tgtggtattc cttaaaacag
     4081 atcccgtaca gcgtttcccc ttctcctact acatatactt tgtaagaagc ctctgcattg
     4141 gctgcctggg cctgggtggt ctgggcggca gtcgtctgtg ccggttctga tgttgtctgg
     4201 actggtggca ctgtctgatc cggcgtttcc ttggttaaag aagcttccac tgtccctgca
     4261 gtagatcccc ctggtgccat tgtctccgga attacctcca ctggcgtttc cccggttgtc
     4321 ctgtttcccg caacagaaga aagatttccc ctggcttcac ggatcactgc ttccggatct
     4381 acagaagcca ccacctgttc catttcatga agccgtttat aattattaaa catggctaca
     4441 ccgcctgcca gcaccatgac tgccagcaca ctgcaaagcc cggaaagtac actgaccgca
     4501 cttctctggc gcacagccgc tgttttatgc tcgtccattt tctttctgaa atcccggatc
     4561 gcccggtcat cggtcttgct ttcttcccgc aggatatctt tccttgcgat catataatcc
     4621 tgcatcatct ggttgcgctc atagtaaata ctgtatccac gcagctggta aaaaccatcg
     4681 gaagaggtaa tataaatagc ttcttccccg tccagtccac tgtttaaata catcagctgg
     4741 tttttccctg taaaatactg gctgtgctgc ctccagtaat ttaaagggct taaggtaacc
     4801 ccttctccgg cgcacaaaaa ccagccctgg actgttcttc gtgggaacat ggtctctacc
     4861 tgctggtatg cctttttcca cgcttcctct gtaaagacca ccttttcccc ttcctgggaa
     4921 aggatctcca tttccattgc tccatctaca aacacatagg gcatgtcctc atggatctcc
     4981 cggttgccaa ggagcagtcc taccctcagg ttctggcctg ccgtaggctt taaccgtttt
     5041 aaatatgtat ttacataatc ttccacataa agccgcagca ctgtatcgcg ttccccgatc
     5101 tgccggatgt tctttggcag tttgggataa agttcatata attcgcccat attttaacac
     5161 cccttttgct gttcttatct gtttacgttc tcacctaaga gtagcaaaag gaatgcaaaa
     5221 attttgtcat attatggctg tattttccca aaatcgttcg acaagggcaa tgctttcgca
     5281 aatgcagtct gccattttca ttaccatgga aagacgcaca ctttgtaaca tcaccggatc
     5341 atggcttccg ggacctccca cgatccctgt tatggaaatg tctcctactt ccatcagttc
     5401 tttggaaact cccagccccg gttgaagagc gcctttcccc aatgttgcaa atccaacgtg
     5461 atccggactt cccacagaag catctatggc cacgatcaca tgatccggaa aatgagtacg
     5521 gatataagct gcataaactt ccaggttcat agcatggacc ggtttgtcta acgtcccgat
     5581 cacagctgct ttttttaatc tgcattttcg cagcttatga cctaccaaag gccccagact
     5641 gtctcctgtg gaacggtccg ttccaataca gagaaatatc actccggcct tctgtttttc
     5701 cgccattgcc tctgcgatca gctggtttaa atttacggca aaccgctccg cttcaaactc
     5761 tcccattgta tcataataag aaatctcgtc ccttcgctgt acccgaaaag acttccacaa
     5821 tctcattgcc aagttctcca ccccggtatc tgtttactat aaatgataaa tactttacct
     5881 gttatcctgt aactggtttc gtttttatag tataggcggt gaaagttggt tttattcttt
     5941 tcagatataa aattcagata caaaaaagag ccttccattg cggaaagccc ttttctcatc
     6001 ctgaatttga tccatttgat caatcaaata atttctcttt ataaactccg gcatcaatca
     6061 gacccttgat tgcgtctaca actgcctgct tgtcctcttt gtaagtaaca ccaaaccact
     6121 tatctggtgt atccagaacg cggacttttg ccttgccttc atgtactaat ttgtcgatga
     6181 tctttggaag caggtattct gccttgatat cacctggctt taagctgtct aagaattccg
     6241 ggaagccctt ttccagctca tttaagaagc ttactggaag tccccacata ttcatggaaa
     6301 caagctgctc tggctgaacc tttacaggat ttcctgcttc gtcagctgca tgaagtccat
     6361 cttccatcat acggatatca taagtctcag ttactttctt taagataccg ttttcctctt
     6421 cgcaaacgcc tctggtaaca gcgccgttgt cgctgagggt attctttaaa atgaaaccgc
     6481 ccatgcagat gtcatataca tcgccatctt ctgtcatttc atttaccata tattcatgga
     6541 tcttgcggaa gccttctttt ccgtagtaat catctgcatt gattaccagg aatgggcagt
     6601 cgatcagtcc ctttaccata agaactgcct ggccggttcc ccatggttta gtacgtcctt
     6661 ctgttacttc atatccttct ggaagagcat ttaattcctg gtatgcatag ataacaggga
     6721 tctgcttttc gatcctgttt ccgatgatct ccttaaagtc tttctccaga tccttacgga
     6781 taataaatac tactttatta aatccggctt cgatagcgtc atgaatggaa taatccatga
     6841 taatctcacc acttggtcct acaggcgcca gctgcttaat gccgccgccg aaacggctgc
     6901 caataccagc tgccatgatc actaatgcgg tctctttcat aattcttctc cttgatttct
     6961 aaatatagtt cagtcattcg ccaaactata agtgtttgta tcggttacac tataacacac
     7021 tttccagtaa aaggcaaaat gttctctcgg aatctttctg tgcctgattt tgtgagaaga
     7081 ttttttgtca gttgtgccca aatttcatca acgtacgcgg tgcgtacgtt gatgaaattc
     7141 gggtgcgact ggcggaaaat cagccacaaa agcaggtgca ggaaagactc cgagagaaca
     7201 taagaggata aggtgggaat tttaatggat ttctatccaa acaatacgtt ttctgccagt
     7261 tttgttaaaa ccttctgcac tctgcacaca tcttcataag gaacaaactc atttggtttg
     7321 tgcgccagct ccagacttcc cggtccataa gacatacagt tatggttcat taatgttcct
     7381 gcgatcactg ctgtgtcggt atagccatta aaggaaccta ccactgcttc ctctcctgtt
     7441 gtctgcttta ctgctgcctt taaatttgct aaaagcttag aatgatcatc tttttccaca
     7501 taaggcctgt caccggtaat gatataactt ccctttactc ccggtatctc ttcctctgct
     7561 ttcctgattg cacggtctac aatggctttg gctgccgctg tatctgtagg cggcaccaga
     7621 cgcatatcga tccatacttt cgcgctgtcc ggcactacat aaggacggta gccgcctgtg
     7681 atctgaccga aagtaacagt agaaattccc aggtcatgat gcttcgggca tgccttgatc
     7741 tcttttctga tactgctgat ggcttctgcc atggctgcaa tggcatcggc tcccttccat
     7801 ggattgcttg catgggcggt aattcctgtt atggtcagtt caaaccaggt tcttcccttg
     7861 tgagccacct ggatctgtcc gtctgtaggc tccgtatcca ggatccagtc atcttttgtg
     7921 acccagccgg ccttaatagc agcttctacg ccacgcataa agtcctcttc atctacagag
     7981 cagatcatgg aaaatggctt ctccggaagt tcgccccttt ctcccacttt ctttaccatg
     8041 tttttaaagg cagttaaagc gcaggcaaga ccggatttca tatcgcaggc tccgcggcca
     8101 tacatttttc cgtcacgtac catagcacct aatggggaaa tatcttcatc ccagccatca
     8161 cccagcataa ccgtatccat atggcagata taaataagtc ccggtccctc tgtttttcca
     8221 gggatctttg ccattacatt ataacgtcca ggcagaactt ccagacgact tacatggatc
     8281 tggtctgcca gcttacctcc aaagtctgtg atctgctttt taaaccagtc tgtgatccac
     8341 agaccgatct cctcttcata ggctcccggg tctgagctgt cgatctgcac cagctgtttt
     8401 gccagttccc aggcttcctg ttcttctctt tccatagtat ttatcttctc cttcctgaat
     8461 gactcatacc tgcgcgctcc cgcgtatcac agtcatttta ttgtggcaat gttagtaaaa
     8521 aaggtgattg cacttgtagc tttaccctac tattataaga gagggttaag aatatgaaaa
     8581 gcccacttaa taaaattaag aaatatgatc aaggagcatt taagtcatta tgatgatgaa
     8641 agaaataaaa agtccatttt ggcgtaccat ttatgaatat ggattaatta ccgtcagcat
     8701 ctggatcatg gtagtaggaa tttatttctt caaattccca aaccacttcg catttggcgg
     8761 aattaccggt ttttccactg tagtcagcca gcttacacac tggtccgcca gtgattttac
     8821 atctgttacc aatacagccc ttctgctgtt aggattttta ttcctgggca aaggctttgg
     8881 attaaagact gtatacgcaa ccatggtcat gtcagtaagc ttatcacttc ttgagcgttt
     8941 ctgcccatta agtgcaccgc tcaccagcca gccgctctta gagctgatgt ttgccatttt
     9001 ccttccggct gtaggaaccg cgatcctctt taatattgga gcatccagcg gcggcaccga
     9061 catcattgcc atgatcttaa agaaatattc cagcatgaac atcggtaccg tgctgatgtt
     9121 agtagacatt gcatccgttg ttgcttccta ctttatcttc ggagtagaga ccggcctgtt
     9181 ctccaccatc ggtcttgcag caaaatcact ggtgatcgat gatgtaattg aaaatatcaa
     9241 tctttgcaaa tgtttcaaca tcatctgtga tgatccaaaa ccaatctgcg atttcattat
     9301 caatggttta aaccgcagtg ctactgtata ccacgcagaa ggcgctttca cccatcacga
     9361 taagaccgtt atcatgacca ccatgaaacg cagccaggca ttaaaacttc gcaattacat
     9421 aaaacgtgta gagccaactg catttatgct gatctccaat tccagcgaga tcatcggaaa
     9481 aggcttcctt gcaaattaag cctcttctgt attcagttcc tttgccactt tttcaaggtc
     9541 tgcccttatg gataatccct gaggacaggc tttttcgcat gccccgcact ttttgcaaag
     9601 gtcagcagat gcatcctgga ccttctgttc tgcgtatgct ttcttcgctt tttcttcctc
     9661 atcaaacatg gcacagttat tccagatgga aaacatttcc ggaatgttta ccccaaatgg
     9721 acatggcata cagtaacggc aggaagtaca gccatttttc acccggctgc ggatcaggtc
     9781 tgctgccttc tcaactgctt cctcttccgc ttctgtcagc ggctgaaatt ttccaaatgt
     9841 ttccagatta tcttctaact gttccataga ggacattccg cttaagatca cttttacatt
     9901 ttcatggctt cctacccagc gcagagccca ggaagcagca gaacggtccg gatctgcctc
     9961 atttaaggct tcttttgcct cttccggcag gtttgccaga cggccgccct ttacaggttc
    10021 catgatcacc ataggaacgc ccatttttgc tgcaagctca tagcctttat ccccggcctg
    10081 tacctgggtg tcaatataat tatactggat ctggcagaaa tcccactgac ggtaagtaag
    10141 gatctctctg aataccgggt atgtatcgtg gaaagaaaag cccagatgac ggatctttcc
    10201 gcttgccttc ttcgcttctg cccattcaat gatcccttct tttaccagtt tttcccatct
    10261 gccggcatct aaggcatgaa gcaggtaata gtcaatgtat ttggtctgga gcctggataa
    10321 ctgctcctta aacagtcttt ccgcatcttc cacagaattc accagccata ccggcagttt
    10381 tgttgccaga taaaagctgt ttcgcggata ttttttcagg gctctgccta aaaatggctc
    10441 gctttctccc ttatgataag gataggccgt atcaatataa ttgatgcctg catttaatgc
    10501 tcttccgatc attgcctctg cctgcgcctc atcaatggtc ccgtcctcat ttaaagggaa
    10561 acgcatacat cccagtccca aaagggatac attctcctct cctccaataa agcgcatggt
    10621 tctgtacttc attctttttc cccctcctgt tattttgagc tgttcttaac tgtttttctt
    10681 cttacatttt acagctgcct gctttcttta ttatagcaaa ttatctgtcg tattactatt
    10741 agattactgt cagataaaat ccccgccatt gtagccattt tggatttatg ttatactata
    10801 agaagcgcgt cagcatgtgt ttgtggatca atttatcaca cactaaccga aaaagagagg
    10861 gaaacactat ggatctgaaa ctacaggtca aacagtcaca ggtcttgtca caacgtatga
    10921 tccagtctgc tgagatcctt cagatgacat cacaggaatt aaatacctac ataaatgatc
    10981 ttgcactgga aaatccggtg atcgacattg tagaaccggc tgccggggac agccagaaag
    11041 agtctattga acagcagcag tggctgaatt cttttaatga ggaaaactat tatttatatc
    11101 agcgtcagaa taatgatgac gactacgatt ttaaagacac ctggaacatt aatacagacg
    11161 aaggggaaac attagctgac tatctatggt cccagctgat cagtgaagat ttttccgaac
    11221 aggaaaccga gatcatccac ttctgtctgg acagtctgga taaccgggga tacctgacgg
    11281 aaacacagga agacattgcc gcttatttta aaacggatac agaaaccatc gaccgtattt
    11341 tagaagagtt aaaatcctta gatcctgcag gggtctgtgc ctttaacctg gaagaatgtc
    11401 taaagcttca gttagaacgt aaaaaaatgc tcacacctgt tatggagtcc ctgatcgaca
    11461 atcacctgga tatggtagcg aaaaaccaga tcccggccat tgccagagcc ctccatcttt
    11521 cttccgggga agcagccggt tactgccaga tcatcaaatc cttaaatcca aaaccgggag
    11581 tatctttcag cagccgtgac cagctgcgct atatcatacc ggatgtgacc attgtaaagt
    11641 tcaaggacca ttttgacatt ttattaaatg agtccatgta cccgggcatt gaagtcaaca
    11701 gctactaccg ccagatgaac caggcacctg actcccagga attaaaagaa taccttggaa
    11761 ataagatccg ccaggccgaa tgggtcaaac agtgcatcac ccagcgcagt aagaccatga
    11821 tggacgtatc cagagtgatc ttagagcgcc aggaagcatt tttctcccat ggtcctaatt
    11881 atttaaaacc actgcgtatg acagatatcg cggaaatctt agggatccat gagtccactg
    11941 tcagccgtgc agtaagcaag aaatatctcc agtgctcctg gggcgtatat cccatgaatt
    12001 tcttcttttc cagaaatgtg ggaacttcaa cttcatcgac agagaagtcc gacgatcctt
    12061 ccaaagcagt gacagcccgt aagatcaaac agctgctcca tgagatcatt gctgaggaaa
    12121 ataaaaagaa gccttacagc gaccgccttc ttggagaaaa gctgacggaa aaggggatct
    12181 ccatctcccg ccgcaccgtt gcaaaatacc gggaagaaga aggaattccc gatgccagcg
    12241 gccggaaaga atatacctga tatagccctc tgaaaaagtt tttaaaaaag tttttaaaaa
    12301 tgcttgacat tttttctaaa aaaaggtatg atatcaccgt tgtcggtaat acaaatcatt
    12361 gctgaccggg atgcccagat agctcagttg gtagagcaga ggactgaaaa tcctcgtgtc
    12421 gttggttcga ttccgacttt gggcactgtg caggtgtagt tcaatggtag aacactagcc
    12481 ttccaagcta gatacgtggg ttcgattccc atcacctgct ttgaaaatta aatattttca
    12541 attatgcgcg agtggctcag gggtggagca ccaccttgcc aaggtggggg ccgcgggttc
    12601 gaatcccgtc tcgcgctttt tttattttct gaagatcctg gttttgcagg atcttttatt
    12661 tttgtgtata aaaatgaaaa agcaggctcc gaagaacctg ccattcatgg tacaataaga
    12721 tcggcacctt tttcctaagc cagcactttt ccttccggga acaggcttct gaatacagtt
    12781 cttactaacg gtcccgcaac gatcagctgg aaaggcagcg ccataataaa gtttttagga
    12841 atgttggtaa gccagatgat cagcagcagg ttccactgtc cggaacggac acaggcttcc
    12901 agtccgccat agaaagacat gatgatcacc attggaacta ccatacaggt agagataacg
    12961 atcactttct ttaacacact gctgtctctg tttacaaaga aacggaaagc aactccctta
    13021 gcaggtccgg atactacaaa ccagtccata cagaaagcaa acacaacggc aattggaaat
    13081 cccagccatc cttcacgaag cacctctgtg ctgaagccac ccatatgaag ggtaacatta
    13141 tacatgctca tccagagcac catggtaaaa cacatcatta cactgtagat cagactttct
    13201 cttttgttct gcggcatttt tcatttctcc tttttcattt tcatgtactt ttttatgttt
    13261 gcatacgaca acctgcctga tcaggcagat gcgcatcgtc agcgcaaaaa agagaccata
    13321 cggggacctg gaaacatgtt ttcatgtttc acggtgactc gtttgatctc tactgctata
    13381 tctcatatat tcgtatgcaa tctcactgcg tcatacgctt gcgcattctg ttttgacaaa
    13441 acactattat agcggctctt ttccggtttc gtaagtactt tttttgtatt ttttacattt
    13501 cgtcactttc tataatacac tatatctgta tacagtatat tttgtacgtc acttacaaca
    13561 caatttttta gccggtcagc cccttcttac tggttctggt tcagatattc catgatcccc
    13621 atatgtaagg tccaggccag ccggtcctgg tactcctctg tttccagctt tttcgcctct
    13681 tcccggttgg ataaaaagcc gcactctgca atgacaatgg gttctttcac atgaaggagg
    13741 agataataat tatcgttggc ttttgcctgc cttttattac tatctcctaa aacatagtca
    13801 aaccgtttct gcaaactttc tgccagtttc tttccccgga cagaagtctt ataataaaat
    13861 acctgtccgc cccggatggc ctcttcatgg tagctgtttt gatggatact gatcaccaga
    13921 tccggctttg cctcttcgat cagctggcag cgctttttca tatccgccat ttttttatga
    13981 gtatctttgc tggaatacag cccggtatcc acatctctgg taagtaccac ctttacatcc
    14041 gaagcctcca aatacgtttt cagcttttta gccactgcca gattaatatc tttttccagc
    14101 tgaccatcga tccctacttt tccgggatct gtgcccccgt gtcccggatc aattacaaca
    14161 accgcctgtt tttgctctcc gggtccattt tctgactgta tatactgcat cccggctgcc
    14221 gccagtcctc ctgcctgcca ggataaaaaa gcaacggttc caagaaggac tactcccata
    14281 agcagctgat aaggagactg tttcatgttc cagacctgct accttctttt tatccagtat
    14341 attccctttt tcctgaaatc agtcaaaaat ccggccggag aataattaac gatcaattta
    14401 cttccagata gttataatcc tttactgcat catctacaat gtattccaga tattcaaaca
    14461 gcaccatagg ataacaatat tcctgccaca tcttatatgc aggtgttgtc gtagcctctt
    14521 ttaccacttc ataggctttt ccgccatagc aggctgcatt taactgtgca tatacctcgg
    14581 acatctgctc cttctgacgc tgggtcagtt ttacaaaaag atcgcctgtt tcctcttcct
    14641 cagagtcctt cattgcatca taagactggt tataaaaaat ggtccttaag gcagccggcg
    14701 cataggtatt aaaattcaac aggttctcgt ctgtactttt atgctttctg gcccattttt
    14761 ccatatctac ctgttgtgtg tgataggaaa agctttcatc atcccggtat tccagcactc
    14821 cgtactggca gggaggtgta gaaagggaac tggtaacaat ctcataaata cctctgtctt
    14881 cctcattcct catataatgc tgtacatgga gatggccgct taaaaacaac ggtacatttt
    14941 cctcttccag acggtttacc agttcttcac tgtgttcaat ggtgcagtct tccacataga
    15001 tcttactttc gtctaacagg ttgtgatggg caacaggaag aaggatcaca ccgtcatccc
    15061 aggcttcatc taactggtcg tcgatccagt cataggtctc tgtttttatc attcccccca
    15121 ctttattcgt aggactgtac tgacagctgt ccagcattaa aaggcgcata tccggtccaa
    15181 gatcataagt ataacttagg gaagccggat ccctgctcag tgcctcatcg tatccaaaat
    15241 cattgtagat ctctgcaaac tcattggcag aaactgcctc tgccatctgt cttgaccttc
    15301 catcaaaaga agctgcattc ctgttattga tatcgtgatt tcccggaata accaccacct
    15361 ggatcccgtc tttttccacc tgttccagct tcttagccag ctccttatgg ctttcttttt
    15421 ctccgtttaa agtcagatcc ccgcttaaaa tgatcacgtc cggagaaaga gcttctacct
    15481 gttcaaaagc agcgtctgtg atctcccaca catagttggt cagttttccg tccccatgtt
    15541 ctaccatgta ctgaaattct tcccctttat ctgtaaggga atctgccaga taatgaatat
    15601 ccgtcatcag gacaatgcgc tttccgtttt tcatcggttc cgcttttgtc tctttttccc
    15661 cgtgtggaaa tggttctata tcttccggat gggcatcggt tggtttctcc gtttcttctt
    15721 ctgtctctcc ggtgggctgt tgttttgttt catcttttgt ctctgtttct gcctgggaag
    15781 catttgcagg ctgtgtttcc ttatttcccg gatacaggct gcatccggcc gcagacatgc
    15841 aaaaagcaga cagcagcgct gccgtcatta taagtctctt ctttttcatc cttcatccct
    15901 ctatttcctg tagcagttca gtcatggctg aactgctact attttacttc atccataatc
    15961 gtccagatat ccgctgtctc aaatcccagc ttccagcagg caattcccgc caggtcatac
    16021 tgattgacca ggtctttttt cagtcccagg gactttgcct cttccatcca gatcttctgg
    16081 accccgtttt cattttcaat ttcaccatag tactggccta ccttgtcctg ccaggtcagt
    16141 tctacattat tttcatctac ccattttttg gcagctgtca ttccataggc ctttgatgta
    16201 gtctttccgt ctttttccgt ccacactctt gtatagaagg gaacactgtt gatcagtttt
    16261 tcctttggaa cttccttaag tgtgccggta ataccatttt ccacatagga aatggaagca
    16321 acggaacctg catctcctcc tgcaaaatgc tcatcatatc ccatgacgat cacataatca
    16381 gccacgattc cctgttcttt ccggttgtag aatgcactgt aaacagcagg aacataatca
    16441 tctactgaca gaaccagtcc cttctgtctg catgatacgg acatttcacg gataaactgt
    16501 acatagtgag gacctgctga ggatttcagg ctttcaaaat ccaggttaaa accgtcaaag
    16561 ccataggtat ctgcctcttt catcagattt tcgatcagct tttttctggt cgcagtagaa
    16621 gaaaata
//
LOCUS       DBMDUA010000047        16128 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2045_length_16128_cov_2.307107, whole genome shotgun sequence.
ACCESSION   DBMDUA010000047 DBMDUA010000000
VERSION     DBMDUA010000047.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 16128)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16128)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16128
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2045_length_16128_cov_2.307107"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(116..607)
                     /locus_tag="ACZPCL_05460"
     CDS             complement(116..607)
                     /locus_tag="ACZPCL_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161956.1"
                     /translation="MKPVIEANFKPFESVRFRQNARLVSKDQLAARQHQAISQYRLKA
                     RQAQNYGISSLESMTCIQNIRQAFDLQDTVQRVHGTANVSGSGNSFGDPFSGSPDNNG
                     LYTLQRGEFEFRVASGDLEYIPALEMTIITSYPEVEFIYTGTPNYVPPKKEPDSPALN
                     VTI"
     gene            complement(682..1803)
                     /locus_tag="ACZPCL_05465"
     CDS             complement(682..1803)
                     /locus_tag="ACZPCL_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HIR1161957.1"
                     /translation="MRVVRSSVYRRYTSSLNDLQSNLNKSMNKVSTGAAYETAADNPL
                     AYYQGKKMDHLYQDAKSKSSILGDIKNRLYQQEQGARDIQKTLSNSKTSMQYVLDSSH
                     NSSKTSVQTKRDALLQDVQSMVSNLNSQYQDFYIYGGNDITTAPFSLSGDGKTLTYTH
                     KYSDGTTKTVNMTMAYDKDKNTYSYHLSDDDLNNLVTAMREQGRVDIGYGDISNRQSL
                     LDTYTGGLNMLTGLTSDSLNAMSDADARDAVKAALETTPVALLSRSVMACDNYINDTT
                     TVDQFTDTMGSVLKDAANTEHTISTIYSDLGNKYSLVETTKTRLDTLSDNLTEEYKDI
                     LGADPYEAITEMYSYQYSYSAALKMGSNLMQSSLFDFIK"
     gene            complement(1826..3502)
                     /locus_tag="ACZPCL_05470"
     CDS             complement(1826..3502)
                     /locus_tag="ACZPCL_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HIR1161958.1"
                     /translation="MVRATFSGFNTALSALQANQKRLDITGQNLSNMNTAGYTRQQLE
                     ASSLNYTNPVSHYSNGNETAVGFGVSMDRVSQIRDPYLDIQYRSQSADCSYTNRLQTA
                     LNSLSKVLDETTISGIRKAFDDVQSTLTSMQDPAKVSDPIYESELRTKMQSVCNLFNQ
                     ASRQITQAEQNEFQRLTGEGSSEQGDVQKINDILRQIGDLNVQIKRNQVAGHPSLELQ
                     DERNLLLDELSGYIPIETRYYKDDAHSGNNAYDYDANGAVIGKKDWPDDLEVSMDYID
                     AQGKSQKLILVNGSDLGADGLTKNYGQLSVLKGDHQTAASVSDPADISIKFEKAPSYK
                     GNGPAATNDVYAELKGIRFEGGSLQASLDMLQKTGTGKVINGSTAVDDVRGYQYYSEK
                     LDQLASTFAKSMNDINNGKKHTDQNLLSNSTDDSTTGITAGNIGISKGWTSGTIHIST
                     DGTNRTDTILDMIAAMKDTKKLNGKTFADYMNNLSTQLASDSSSNQTVLKTGTTVLNS
                     ILDSRDSLSGVSLDEEATNMMAYVSAYNAASRLMTALDEVLNTLISNTGA"
     gene            complement(3554..3982)
                     /gene="flgN"
                     /locus_tag="ACZPCL_05475"
     CDS             complement(3554..3982)
                     /gene="flgN"
                     /locus_tag="ACZPCL_05475"
                     /inference="COORDINATES: protein motif:HMM:NF016985.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HIR1161959.1"
                     /translation="MRAPESLIPVLEQLCTVLEQLCQVEADKLTVISADHPDQLESLL
                     NDENMLLLQLRGLDKKRSDLLRKSGLEGLTFRQILMQEDSDKAVALLSPILERLSSQA
                     EKLKKVKGGTDRLIRIRMKQLEQRLSGAKQSDPHVYDKYV"
     gene            complement(3979..4290)
                     /locus_tag="ACZPCL_05480"
     CDS             complement(3979..4290)
                     /locus_tag="ACZPCL_05480"
                     /inference="COORDINATES: protein motif:HMM:NF016223.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HIR1161960.1"
                     /translation="MKIQDSIQEQIHMKHVSIAERNVALSNNISAKPADLDRASFEYT
                     EGISADETLFARSLAHSITEDVCREKGVSVEKVAQLKKQVEEGTYELDPMAIARAMYN
                     L"
     gene            4521..5945
                     /locus_tag="ACZPCL_05485"
     CDS             4521..5945
                     /locus_tag="ACZPCL_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161961.1"
                     /translation="MSNILQVTDPSRYIDNNRNVHTGEEQQSALNSSQIQNPSDPSRV
                     VRADGQKSGTAGDQESESGFGIRNYNGNYTDFLQKLGQENEIKDSLGRILFRDGAFIA
                     KGDEDLGKMVQQLLDSASFEDPGQLLDFLKMQGQTKFSGTFFENLRTLLQQEGNRGLQ
                     ETALKFLQVFNNYSSGEHLLGQMKELTDEIKGMLYTSVRQEYASILDQLDWKAENGET
                     KENSQVLFQKLVPFLSRYISRTHDYGNVRSSVIQLILYGARYENGSSALLRSLFEQMA
                     VNRDFKRFFETEPEELYAVLTGQEGKKRNIYGDLFGSLVERGAKGEAGLEHVQDFYQL
                     MRGMLVNESVYMPVNHFILPFQYQGKEAVSEFWIDPDAEKKEEEKDGGRKIRLLVDFE
                     IRGLGDFQLLADLQDHKISMELGVPKNLSAGAGEIQAKLQEICTRNGMRVKMMQVQER
                     KAHYRLLDVFPKIRKKENGINVSV"
     gene            5932..6243
                     /locus_tag="ACZPCL_05490"
     CDS             5932..6243
                     /locus_tag="ACZPCL_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HIR1161962.1"
                     /translation="MSAFERLLEQKAVALQYSPEKDSAPVIVASGMGYMAEKITEAAR
                     KSGVPVYEDDSLATLLSRLQLGAAVPEELYQAIIEIYIYFLGYVPSPEEKENEEKAKN
                     T"
     gene            6218..7630
                     /locus_tag="ACZPCL_05495"
     CDS             6218..7630
                     /locus_tag="ACZPCL_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR1161963.1"
                     /translation="MKKKPKIPDRKFRHRHKMLLLLLSGALLFQWGKIDAFAAAKEIK
                     TVNIRVISKLEAGSKLPDIKLETTTVSDGEVAVDAGGSKYSVSEAEWTDKSSNELKAS
                     EEPQMKVTLEPEDVSEDYFVSSYKKANVKISGGTFVSARRDGDDLVVTLRVNGIKGDY
                     AAPEDAWWNEKSLGQAKWEKPDNTSGYYEVQLYRGKSKVYSVSQTSAVQYNFYPYMTK
                     TGEYTFKVRTVPGTDSQKKYGGKSEWAESGELSITDRYVSDGKGQQSKNPSAKSGTTD
                     TVGWVKKDNVWNYRFPDGSICRGAWQSVNGYWYYFDVNGTMLTGWQKVANDWYYLYDT
                     GEMAVGWAKINGQWYYFWPLTENGHTQGTMAYGGWKIIGADYYFFREDGSLYTGWLEQ
                     NGSWYYLNTLDNSLQGAMFTGWLIREGKTYFLDADGVMVTGWYQVDGNWYYFWPDSGE
                     MAKNQYINGFYVNEDGIWYR"
     gene            7650..8798
                     /locus_tag="ACZPCL_05500"
     CDS             7650..8798
                     /locus_tag="ACZPCL_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR1161964.1"
                     /translation="MKMIRKILLCAGTGLLLGTGSLQSVPGGIFHMYTVYTAYGAESS
                     IIETMSIKFDTTFGDPEEIPQPQITISGSGVSIGDIYYKTEYENWKPGKKVRMEITVD
                     AAQGKYFPVSLGRSKCRVTGADLVSAKALDNTTMQVKVDYTPVTVLGDTALAGWSSLD
                     SEKAVWKKVDYAPGYTLTLYGNDKVVKRMTVTGNSVSLKEYMTDPDKIYYYQVKAVPV
                     TAEQKKYLKEGEFVTSQEQDIDDSEEEEKEKKHAATSQSCSTGPGAAGSLKGDNFVMP
                     DGTLAVNTWKLVGGIWYYFNAEGNRTRGWFLYGGKWYYFDGNGCMKTGWVNTGNGKWY
                     YLNPDGDMKTGWLYDKNIWYYLNNDGSMAVNCSQDGWYLGADGAGRKE"
     gene            8804..9535
                     /locus_tag="ACZPCL_05505"
     CDS             8804..9535
                     /locus_tag="ACZPCL_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR1161965.1"
                     /translation="MSLAPITGDGGKLAAQAADQTQKTTPVQEFQGILKNGLNTIKED
                     MDSIFEEASALYQIPSKLLRAVAKAESGFNPKAVSKAGAMGVMQLMPGTARSLGVSDP
                     YNARQNILGGAKYLKQNLNRFGGDVSLALAAYNAGPGSVTKYGGIPPYKETQNYVKKI
                     MADYTGNNTILAGRTVSTGTYGKTSGSTAVSSLIGSGLTGSSLGTLLAAGKGENLSGE
                     DWSNMVQILRLQMMMNMGKDTGTLI"
     gene            9663..10529
                     /locus_tag="ACZPCL_05510"
     CDS             9663..10529
                     /locus_tag="ACZPCL_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HIR1161966.1"
                     /translation="MQVQNNIASINAARNQGIETGKLKKSLEKLSSGFRIVRAGDDAA
                     GLTISEGMRSRINSLNQAMRNIDDGIGLTNVGDGALTEVHSMLQRLKTLALKSANGTY
                     DQSNRETLNMEKDQILEEIDRIGSTTKFGEISLFSRADANTNTDPPLWQPPELDDTIP
                     LQIGGSTQAGEVLDVERYYIGSRELQLDQTDFSDVEKGQKSVGYIENAIEAVSKVRAS
                     FGAAYMHLDHTHNNLSVTSENMQAAESQIRDTNMAEEITKYTSSNIVLQSSNMMLTNA
                     NNLPQTILELLK"
     gene            10694..11518
                     /locus_tag="ACZPCL_05515"
     CDS             10694..11518
                     /locus_tag="ACZPCL_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin N-terminal helical domain-containing
                     protein"
                     /protein_id="HIR1161967.1"
                     /translation="MVIQHNIAAINSYRNLSTNQSALSKNLEKLSSGYRINRAGDDAA
                     GLAISETMRSQINGLNQAVNNANDAVGLIQTAEGAMTETHSMLQRMKTLTTQAANGTY
                     TKTARDNIKAEIDALNKEITRIATTTEFNGEKPLSPAKAGQALTFFIGASADTTNAMT
                     VGQQVMTATALTIDKIAVDTTTNAFKAMKSVDAAIEKVSSYRATLGAAQNRLEHTVNN
                     LKVTSENITSAESRIRDTDMADEITAYTKNNILLQAAQSMLSQSNAMPQGVLSMLQ"
     gene            11639..12940
                     /locus_tag="ACZPCL_05520"
     CDS             11639..12940
                     /locus_tag="ACZPCL_05520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family 2 protein"
                     /protein_id="HIR1161968.1"
                     /translation="MIVKNEEKNIRRALSWGKDIMCEQIVVDTGSSDRTVEIAREMGA
                     KIFFFPWINDFAAAKNFAIDQAKGDWIAFLDADESFTPEDTKKIPEILEYVGDDVDGL
                     LTGIVDLDENNNITSGGTMIRFFVNRPDLRYVGKIHEHLVRKGRSGLHLTDATKQLAF
                     LHTGYQEQEKKDKSKFERNLNIILEILKQDPENCDYLGYLGDTYSSDGKNEEARDAYK
                     KAVAAMPEKLEVYDQRSSYTYTNLLRVSQCLNAPEAEVEAIYKEAVEKLPKEPDFDCV
                     MGDWYWRKGQYEKAVQMYELAIAKLETYGTVNRGIITTSMLIQMYECLGEGCRKLGDY
                     QKAVRYCVIVLNTDHKDMNALLTLINCFTESNVSAEEVIQFLGKIYDYSDIKDKVILL
                     RVAMAMGRKDLERMFRGILLPQEREEFDAAMETAQSGAPLS"
     gene            complement(12877..13791)
                     /locus_tag="ACZPCL_05525"
     CDS             complement(12877..13791)
                     /locus_tag="ACZPCL_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR1161969.1"
                     /translation="MIQLSITGTVSVITASLSFYFCPGFSSQVIKDARSGISFVQTAS
                     SQKFHELEDFLKKQTQPKPKSQKQTELSEDTSGTHGIPDTSSVTSKVSPVILGKAENL
                     GMEAKPGSDGNSYDAAILDTSMGPMYYYNQGDTRWSSYLYGGSDPMLQYGCGPTAAAM
                     LISSFTNGGEAITPVTIADWSAANGYYAPQSGSYHSLIPAVLTAYGFHVESVTDHSPQ
                     NAASLLSSGHILVALMGKGSLTQNGHFVLITKLLPDGNVSIADPNSFDNSTCEWELPF
                     LMEELKKVYDSGAPLWAVSMAASNSSLS"
     gene            complement(13891..14973)
                     /locus_tag="ACZPCL_05530"
     CDS             complement(13891..14973)
                     /locus_tag="ACZPCL_05530"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate
                     methylesterase/protein-glutamine glutaminase"
                     /protein_id="HIR1161970.1"
                     /translation="MDHKLKLMVVDDSPLFRTWLIRSLSADDRFQIVGYAVNALDAQE
                     KIPLLQPDMLTLDIEMPGMSGIDFLKQLLPVHPIPVILVSSLNLRVFDALEAGAVDFV
                     RKPDEANGLSKEIFLSMLRSKLLVAAASHVHLPSVPASPQAAKKRIVSPLTAAGNPFL
                     NRPKTSAAASQETVIAIGASTGGTEAILEVVKNFPENTPGVVITQHMPAGFTAMYAER
                     LNRICKMEVREAKNGDRIHKGLMLLAPGGLQMRVVPLGNSYAVSVQEGEKVNGHRPSV
                     DVLFDSVALHVREHAIGVLLTGMGADGAAGLLRMRKNGAYTIGQDKNSCVVYGMPMEA
                     HKIGAVCQQCPLNTIGQTVLARVSLR"
     gene            complement(15031..15843)
                     /locus_tag="ACZPCL_05535"
     CDS             complement(15031..15843)
                     /locus_tag="ACZPCL_05535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HIR1161971.1"
                     /translation="MLTLSDGDFNRLYTYIQQRYGINLSHKKQLITSRLTNMLQQKGF
                     HSFTEYVDEIISGKDPAMVSVMLNKLTTNYTYFMREKEHFDFLQKHILPELANKHSMD
                     RSIAIWSAGCSSGEEPYNISMYLKEYFSAIPGSWDTRILATDISQRVLDSAMEPRYSL
                     ESLKELPPSWQERYFVPIGDKTYTVSDALRKNVIFRSFNLMDPIHFRKKFDLIFCRNV
                     MIYFDRPTKAALVRRFYDATAPGGYLFIGHSESLDKGSCPFSYIEPAIYRKI"
     gene            complement(16009..16122)
                     /locus_tag="ACZPCL_05540"
     CDS             complement(16009..16122)
                     /locus_tag="ACZPCL_05540"
                     /inference="COORDINATES: protein motif:HMM:NF046702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BFO_2992 family lipoprotein"
                     /protein_id="HIR1161972.1"
                     /translation="MSFDYDPSLPENTNAEQKGYYTVTIQGETAVRWYDHT"
BASE COUNT         4750 a         3263 c         3898 g         4217 t
ORIGIN      
        1 gggaaaaacc aaataaacca tctggaaata caaccagctc gtctttcgta taatccatct
       61 cgccgtaata ttttgtatca atcttcatgc ttgtataagc ctccggggtc agtttttata
      121 tggtaacatt aagagcaggg gaatccggct ctttttttgg tggaacataa ttgggggtgc
      181 cggtatatat aaattctact tcagggtaag aggttatgat ggtcatttcc agggctggta
      241 tgtattccag atcgccagat gccacacgaa attcaaattc acctctctga agggtataca
      301 acccattatt gtcagggctg ccagaaaatg gatcaccaaa gctgtttcct gatccagaaa
      361 cgtttgctgt gccatgtact ctttggacag tatcctggag atcaaaagct tgtctgatat
      421 tctggataca ggtcatgctt tccagagaac tgatcccgta attttgagcc tgacgtgctt
      481 tcaggcggta ttgggaaata gcctgatgct gtcttgctgc cagctggtcc tttgaaacca
      541 gcctggcatt ttgacggaaa cggacagatt caaaaggttt aaaatttgct tcaatcacag
      601 gtttcatata tcttcacctt cttcagacct gaaaatggaa agttattttt ttgcttgttt
      661 tttgtaaaca atgtaccggt atcattttat aaaatcaaaa agagatgact gcatcaggtt
      721 tgaccccatt tttaaagcag cagaataaga atactggtaa gagtacattt ctgtgatggc
      781 ttcataagga tcagctccga gaatgtcttt gtattcctct gtcaggttgt cgcttaaagt
      841 atcaagacgg gtttttgtag tttctaccag ggaatattta ttgccaaggt cgctgtaaat
      901 ggtgctgatg gtatgctctg tattagctgc atctttaagt acactgccca tagtatctgt
      961 aaattgatca acggtcgtag tatcattgat ataattatca caggccataa ccgaacggct
     1021 gagaagtgcc acaggagtgg tttccagggc agctttgacc gcatcccttg catccgcatc
     1081 agacatggca tttaaggaat ctgaagtaag tcccgtaagc atattcagtc ctcctgtata
     1141 ggtatctaaa agagactggc ggttggaaat atcgccataa ccgatatcta cccggccttg
     1201 ttctctcata gctgtcacaa ggttgttcag gtcatcatcg cttaaatgat aactgtaagt
     1261 atttttatct ttatcataag ccatggtcat attgaccgtc tttgtggtac cgtctgaata
     1321 cttatgggta taagtaaggg tttttccatc tccgcttaaa gaaaacggag ctgttgtaat
     1381 atcatttccg ccgtaaatat aaaaatcctg atactgactg ttcagattac tgaccatgct
     1441 ctgcacatcc tgaagaagag catctctttt tgtctggaca gaagtttttg agctgttgtg
     1501 ggaggaatcc agcacatact gcatggaagt cttactgttg cttaaagtct tctggatatc
     1561 ccttgcgccc tgctcctgtt ggtacagacg gtttttaata tcacctaaaa tagaactttt
     1621 actctttgca tcctgatata aatgatccat tttctttccc tggtaataag caagaggatt
     1681 atcagcagct gtttcataag cagcgccggt agaaaccttg ttcatgcttt tgttcagatt
     1741 gctttgaaga tcatttaaag aagaagtata tctgcggtaa acagatgaac gtacaactct
     1801 cataatttat taatcctcct tatatttatg caccagtatt actgatcagg gtattcaaca
     1861 cttcatcaag ggcagtcatc agccgggaag cagcattata ggctgaaacg taggccatca
     1921 tattggttgc ttcttcatcc aggctgacac cggaaagaga atccctggag tccaggatac
     1981 tgtttaaaac agttgtgcct gtttttaata cggtctggtt ggaactggaa tcagaggcca
     2041 gctgggtgga aaggttattc atataatcag caaatgtttt tccgttcaat ttcttggtat
     2101 ccttcatagc tgcgatcata tcaaggattg tatctgtgcg gttggtgccg tctgtagaaa
     2161 tatggatcgt tccactggtc cagcctttgc tgataccgat atttccggct gttataccag
     2221 ttgtggagtc atcggtggaa ttagataaaa gattctgatc cgtgtgcttt ttgccattgt
     2281 tgatgtcatt catggatttt gcgaaagtgg aagctaactg atccagtttt tctgagtaat
     2341 actggtatcc acggacatca tccacagcag tagatccatt tatcaccttg ccggtacctg
     2401 ttttctggag catatccagg cttgcctgaa gagagccgcc ttcaaaacgg atccctttta
     2461 actctgcgta tacatcattg gtagctgccg gtccgtttcc tttatagctg ggggcttttt
     2521 caaacttgat ggaaatgtct gcaggatcag aaacagaagc agctgtctgg tgatcgcctt
     2581 ttaacacact taactgaccg tagttttttg tgagaccgtc agcaccaagg tcagatccgt
     2641 ttaccaggat cagcttctgg ctctttccct gggcatcaat gtaatccatg ctcacttcca
     2701 ggtcatcagg ccaatccttc ttgccgatca cagcaccatt tgcatcatag tcataggcgt
     2761 tattcccgga atgggcatca tccttgtaat atctggtttc aataggaata taaccggaaa
     2821 gctcatccag gagaagatta cgttcatcct gcagttccag agaaggatga ccggcaacct
     2881 ggttacgttt gatctgcacg ttcaggtcgc cgatctgacg gaggatgtca ttgatcttct
     2941 gcacatcgcc ctgttcactg ctgccttccc ctgtgagcct ttgaaattca ttctgttcgg
     3001 cctgtgtgat ctgtctggat gcctggttaa aaagattaca tacagactgc attttagttc
     3061 gcagctcact ttcatagatg ggatcggata cctttgcagg atcctgcatg cttgtaaggg
     3121 tagactggac atcatcaaat gccttacgga tgccggaaat ggttgtttcg tccagtacct
     3181 ttgacaggga attaagggca gtctgtaaac gattggtata gctgcagtca gcagactggc
     3241 tgcggtactg gatatccaga taaggatctc tgatctgtga gacccggtcc atgcttaccc
     3301 caaagcctac tgcagtttca ttcccattag aataatggga aacaggattc gtataattaa
     3361 ggctggatgc ctctaactgc tgcctggtat atccggcagt attcatattt gacaaattct
     3421 gtcctgtaat atccagacgt ttctggttgg cctggagagc ggacagagcg gtattaaaac
     3481 cggaaaaagt tgctcttacc ataaaatatt attacctcca aaatataagt atcctgcagt
     3541 ccccacagtt ttatcagaca tatttgtcat aaacatgagg atcgctctgc ttcgcaccgg
     3601 aaaggcgttg ttctaactgc ttcatgcgga tccgtatcag acggtctgtg ccacctttta
     3661 cctttttcag cttttcagcc tggctggata accgttccag gatcggcgag agaagtgcca
     3721 cagccttatc gctgtcctcc tgcatcaaaa tctggcggaa agtaaggccc tcaagacccg
     3781 acttgcgaag aagatcactt cgttttttat ctagtccgcg cagctgcagt aaaagcatgt
     3841 tttcatcatt taaaagagat tccagctgat cgggatgatc agctgaaata acggttagtt
     3901 tatcagcctc aacctggcat agctgttcta atacagtaca cagctgttct aataccggga
     3961 tcaggctttc aggcgccctc atagattata catggccctt gcaatggcca tgggatccag
     4021 ttcgtaggtt ccttcctcaa cctgtttttt cagttgggca accttttcaa cggaaacgcc
     4081 tttctcgcgg cagacatctt ctgtaatgct gtgagccaga gagcgtgcaa aaagtgtttc
     4141 atcagccgaa ataccttcgg tgtattcaaa actggctcgg tcaagatcag ccggttttgc
     4201 agaaatatta ttagacaaag caacattacg ctctgctatt gatacatgtt tcatgtgtat
     4261 ttgctcctgg atagaatctt gaattttcat acaattattc ctccccttaa aatgaaacgg
     4321 caggatcaca agggatccag caatttttcc cctgcttact gaatctatgt aaattatcgt
     4381 cttttttctt gaaaatataa gaaaaataat aattataaaa aacgggaaaa cgccgattaa
     4441 atatatatgg aatatggaag tatgtaggat aaacttatat tattataata ttatagaatc
     4501 aaaaagaaaa ggaataaatc atgtctaata tattacaggt aacagatcct tctagatata
     4561 tagataataa tagaaatgtc catacaggcg aggagcagca aagcgccctg aacagttccc
     4621 agatccagaa tccttctgac ccatctcgtg tagtgcgggc agatggacag aaaagcggca
     4681 ccgctggtga ccaggaaagt gaaagtggct tcggcatccg caattataat ggaaattata
     4741 ctgactttct tcagaagctg gggcaggaaa atgaaataaa ggattccctg ggccgaatcc
     4801 tgttccggga tggagccttt atcgctaaag gcgatgaaga tctgggtaaa atggtgcagc
     4861 agctgctgga ttctgccagt tttgaagatc ctggccagct actggatttt ttaaaaatgc
     4921 agggacagac aaaattttcc ggtactttct ttgaaaacct gagaactctt ttgcaacagg
     4981 aaggaaacag gggactgcag gaaacagccc ttaaattcct tcaggtattc aacaattatt
     5041 ccagcggtga gcatctttta ggacagatga aggaactgac agatgaaata aaaggaatgc
     5101 tttacacctc tgtgagacag gaatacgcat cgattctgga tcagctggac tggaaggcag
     5161 aaaatgggga aacgaaggaa aattctcagg ttctgtttca gaagctggtc ccctttcttt
     5221 caagatatat ttcccgtacc catgattatg gcaatgtaag aagcagcgtg atccagttga
     5281 tcctttatgg ggcgcgttat gaaaacggaa gcagtgccct tctaaggagt ctttttgaac
     5341 aaatggcagt taatagggat tttaaacgtt tttttgaaac agagcctgaa gaactgtatg
     5401 ctgtacttac cggccaggaa ggaaaaaaaa gaaatattta tggagatctt tttggctcac
     5461 ttgtggaaag aggggccaaa ggagaagccg ggctggaaca tgtacaggat ttttaccagc
     5521 tgatgcgtgg gatgctggta aacgaaagcg tatatatgcc tgtaaatcat tttatccttc
     5581 ctttccaata tcagggaaaa gaggctgtat ctgaattttg gatcgatccg gatgcagaga
     5641 aaaaagaaga agaaaaagat ggaggcagga aaatacgcct tctggtggac tttgagatca
     5701 ggggattggg agatttccag cttctggcag atctccagga tcataaaata tccatggagc
     5761 tgggagttcc taaaaatctt tctgccggtg ccggagagat acaggcaaag cttcaggaaa
     5821 tatgtaccag aaacgggatg cgcgtgaaaa tgatgcaggt acaggaaaga aaagctcatt
     5881 accgtcttct ggatgttttc ccaaagatta gaaaaaagga gaatggaatc aatgtcagcg
     5941 tttgagcggt tattagaaca aaaggcagtt gcacttcaat attcaccgga aaaggacagc
     6001 gccccggtga tcgttgcttc cggaatggga tatatggcag aaaagattac agaagcagcc
     6061 agaaagtctg gggttccggt ttatgaggat gattcccttg caactctttt aagcagatta
     6121 cagctgggag cggcagtgcc ggaagaatta tatcaggcga tcatagagat ctatatttac
     6181 tttttaggat atgtgccgtc accggaggaa aaagaaaatg aagaaaaagc caaaaatacc
     6241 tgacagaaag ttcagacaca gacataaaat gcttctgctc ctcctttcag gagccctgct
     6301 gtttcagtgg ggaaagatag atgcttttgc agcagcaaaa gagataaaaa cagtaaatat
     6361 ccgtgttata tccaagctgg aagcaggcag caagcttccg gatataaaac tggaaacgac
     6421 cacggtttca gatggggaag tagcagtaga tgccggcgga agtaaataca gcgtttccga
     6481 ggcagaatgg acggataaaa gctccaatga actgaaggct tctgaagaac ctcagatgaa
     6541 agtcaccctg gaaccggaag atgtcagcga agattatttt gtttccagct ataaaaaagc
     6601 caatgtaaag atcagcgggg gaacctttgt gtctgccagg agagacgggg atgatcttgt
     6661 agttaccctg cgggtaaacg ggatcaaagg agactatgct gctccggaag atgcctggtg
     6721 gaatgaaaaa agcctgggac aggctaaatg ggaaaaaccg gataacacct ccggttatta
     6781 cgaggtacag ctttacagag gaaaatcgaa agtttacagc gtctcccaga ccagtgcggt
     6841 ccagtataat ttttatccat atatgacaaa aacaggagag tataccttta aggtgcgcac
     6901 agtaccggga acagattccc agaaaaaata tggtggaaaa agtgaatggg cagaatccgg
     6961 ggagctttcc attacagacc ggtatgtatc agatggtaaa ggacagcagt caaaaaatcc
     7021 ttcggcaaag tccggaacca cagatacagt aggctgggta aaaaaagaca atgtatggaa
     7081 ttacagattt ccggatggct ctatatgtcg gggtgcatgg cagtcggtca acggatactg
     7141 gtattatttt gatgttaatg gcacgatgct taccggctgg cagaaggtag caaatgactg
     7201 gtattacctg tatgataccg gggaaatggc ggtcggatgg gcaaagatca atggacagtg
     7261 gtattatttc tggccattaa cggaaaatgg gcatacacag ggaaccatgg cttatggagg
     7321 ctggaagatc ataggggcgg attattactt tttcagagaa gacggatcct tatataccgg
     7381 ctggctggaa cagaatggtt cctggtatta cttaaacaca cttgacaaca gccttcaggg
     7441 agccatgttt actggctggc tgatccggga agggaaaact tatttcctgg atgctgacgg
     7501 agttatggtt accggctggt atcaggttga cgggaattgg tattatttct ggccggattc
     7561 aggtgaaatg gcaaaaaacc agtatatcaa tggattctat gttaatgagg acgggatctg
     7621 gtacaggtaa agatatgtga ggaatggata tgaaaatgat cagaaaaata ctcctttgtg
     7681 ccggaacagg actgctgctt ggaacaggaa gtttacagtc tgtaccaggg ggaatctttc
     7741 atatgtacac agtgtataca gcttatgggg cagagagcag catcatcgag accatgagta
     7801 ttaaatttga caccactttt ggagatccgg aggaaatacc acagccccag atcaccatca
     7861 gcggatcagg ggtatccatt ggggatattt attacaaaac agagtatgaa aactggaaac
     7921 ctggtaaaaa ggtacggatg gagatcactg tggatgctgc acagggaaag tattttcctg
     7981 tgtcccttgg acgatccaaa tgccgtgtga ccggggcgga tcttgtatcg gcgaaagccc
     8041 tggataatac caccatgcag gtaaaagtgg actatacgcc ggtcactgtt ctgggtgaca
     8101 cagctttagc aggctggagt tcacttgact cagaaaaggc agtgtggaag aaggtagact
     8161 atgctccggg ctataccctg actttatatg gaaatgataa agtggtgaaa cggatgacag
     8221 ttacaggtaa ctctgtcagc ttaaaggagt acatgacaga tccggacaag atctactatt
     8281 atcaggtaaa agcggtcccg gttacggcag aacagaaaaa atacttaaaa gaaggggaat
     8341 ttgttacatc acaggaacag gatatagatg acagtgagga agaggagaaa gaaaaaaaac
     8401 atgcagcaac ttcccagagc tgcagtactg gtccaggcgc agcaggttct ctaaagggag
     8461 acaactttgt tatgccggat ggtactctgg cagtcaatac ctggaagctg gtgggaggta
     8521 tatggtacta ctttaacgct gaaggaaacc gtaccagggg atggttcctg tatggtggaa
     8581 aatggtatta ttttgatgga aatggatgta tgaagaccgg atgggtgaac acaggaaacg
     8641 gaaaatggta ttacttaaac ccggatgggg atatgaagac cggttggctg tatgataaaa
     8701 atatctggta ttatttaaat aacgatggat ctatggcagt aaactgttct caggatggct
     8761 ggtacctggg agcagacggc gcaggccgaa aggagtaaga aatatgagtt tagcaccgat
     8821 aacaggagat gggggaaagt tagctgcaca ggcagcggat cagacacaga agacaacacc
     8881 ggtccaggaa tttcagggga tactgaaaaa tgggctgaat accataaaag aagatatgga
     8941 ttctattttt gaagaagctt ctgctttata tcagatccct tctaaacttc tcagggcggt
     9001 ggcaaaggcg gagtcagggt ttaatccaaa ggctgtatcc aaagcagggg ctatgggcgt
     9061 gatgcagctg atgccgggaa cagcccgttc tcttggagta agcgatcctt acaatgccag
     9121 acaaaatatc cttggagggg caaaatactt aaaacaaaat ctgaaccggt tcggaggaga
     9181 cgtaagcctt gcattggcag cctataacgc tggaccgggc agtgtgacca aatacggagg
     9241 catcccacca tataaagaaa cccagaatta tgtaaaaaag atcatggcag attatacggg
     9301 aaacaatacg atcctggcag gacgtacggt gagtaccggg acatatggga aaacatccgg
     9361 cagtacagca gtgagcagcc ttataggaag tggccttaca ggaagcagcc ttggtacact
     9421 tctggcagca ggaaagggag agaacctttc cggggaagac tggtctaata tggtgcagat
     9481 cctccgtctt cagatgatga tgaatatggg taaggatacg ggtacgctga tctgacagac
     9541 agataccgga ttgctgcgtg ctttgcactc ccgcaatttc ggccttttga ccctggtatc
     9601 aataaaataa ggcattttgc cgataataaa aacaaatggt tatttaagga aggtgaaaca
     9661 tcatgcaggt gcagaataac atagcatcca taaatgctgc cagaaatcag gggatcgaaa
     9721 caggaaagct gaaaaagtcc cttgaaaagc tgtcttccgg ctttcggatc gtccgtgcag
     9781 gagatgatgc agctggcctg acgatctcgg aaggtatgcg cagcaggata aacagcttga
     9841 accaggcaat gagaaacatt gatgacggca tcggacttac caatgtagga gatggcgctt
     9901 tgacagaggt tcattccatg ctccagagac tgaagactct ggctttaaaa tctgcaaatg
     9961 gaacttacga tcaatccaac agggaaacct tgaacatgga aaaagaccag atccttgagg
    10021 agattgacag gattggttct accactaaat ttggagagat ctccctgttt tccagggcag
    10081 atgccaatac aaatacagat ccaccgcttt ggcagccgcc agagctggac gatacgatcc
    10141 cgctccagat cggcggttct actcaggctg gagaggttct ggatgtagaa cgctattata
    10201 taggaagcag ggaactacag ctggatcaga cagatttttc cgatgtagaa aagggacaaa
    10261 aatctgtggg atacattgaa aacgcgatag aggctgtttc taaagtcagg gcatcctttg
    10321 gagctgctta tatgcatctg gatcacaccc acaataattt aagtgttaca agtgaaaaca
    10381 tgcaggcggc agaaagccag atccgtgata ccaatatggc agaagagatc acaaaatata
    10441 ccagcagtaa catcgtcctc cagtcctcta atatgatgct tacaaatgca aataatctcc
    10501 ctcagaccat acttgaactt ttaaaataaa aatgaaagaa ataatgaaaa acaggcttat
    10561 gtttttttaa cataagacga tatatataca tagaaagaaa attctgctgt tccagaaagg
    10621 gcccttggga caatagaaga cagaggaaag gagtccactg ccatattttc ttttatataa
    10681 ggaggaattc attatggtaa tccagcataa tattgcagcg atcaattctt acagaaactt
    10741 aagcaccaac cagagcgcac tgagcaaaaa cttagagaag ctgtcttccg gttataggat
    10801 caaccgtgca ggcgatgacg cagcaggtct ggctatttcc gagaccatgc gttcccagat
    10861 caatggttta aaccaggctg taaacaatgc aaacgatgca gttggtctga tccagacagc
    10921 agaaggtgct atgacagaga cccactccat gcttcagcgt atgaagactc tgactaccca
    10981 ggcagcaaat ggtacttata ccaaaactgc aagagacaac atcaaggctg agattgatgc
    11041 actgaataaa gaaattaccc gtatcgctac tactacagag ttcaacggtg agaaaccttt
    11101 aagtcctgca aaagcaggtc aggcacttac cttctttatc ggtgcttcgg cagatacgac
    11161 caatgcaatg actgttggac agcaggttat gacagcgaca gcattgacta ttgacaagat
    11221 tgcggtagat actacaacta atgcattcaa ggcaatgaag tctgtagatg ctgcaattga
    11281 gaaggtttct tcttaccgtg ctacgctcgg tgctgcccag aaccgtctgg agcatactgt
    11341 aaacaacctg aaggtaactt ctgaaaatat tacatcagcg gaaagccgta tccgtgatac
    11401 tgatatggca gatgagatca cagcttatac aaagaacaat attcttttac aggctgctca
    11461 gtctatgctt tcccagtcca acgctatgcc tcagggtgta ttaagcatgc tgcagtaatc
    11521 ttgaaattga ttctgcatct tttaggggat ctgctttatg cagattcccc ttttttttaa
    11581 tatattttgg aacataaaaa gaaggtaaca gatgatgaaa aaaaacatat cacaatgtat
    11641 gatcgtgaaa aatgaagaaa aaaatatccg acgcgctctt tcatggggga aggatattat
    11701 gtgtgaacag atcgtggtag atacaggaag cagtgaccgt accgtggaga ttgccaggga
    11761 aatgggggca aagatatttt ttttcccatg gatcaatgat tttgctgctg ccaaaaattt
    11821 tgccattgac caggcaaaag gagactggat cgcctttctt gatgcggatg aaagctttac
    11881 accggaggat acaaagaaga ttccggagat attggaatat gttggagacg atgtagatgg
    11941 tcttcttaca gggatcgtag atctggatga aaataacaat atcacaagcg gcggtactat
    12001 gatccgcttt tttgtaaacc ggccggacct gcgctatgta ggaaaaatac atgaacacct
    12061 ggtcagaaaa ggaagatccg gattgcatct gacagatgct acaaagcagc tggctttcct
    12121 acataccgga taccaggaac aggagaagaa ggataagtcc aaatttgaaa ggaatctgaa
    12181 cattatcctg gagatcctga agcaggatcc tgaaaattgt gattatcttg gatatctggg
    12241 agatacctat tcttcagacg gaaaaaatga agaagcaagg gatgcataca aaaaggcggt
    12301 ggcagccatg ccggaaaaac tggaggtata tgatcagcga agttcttata cctatacaaa
    12361 cctgttaagg gtctcccagt gcctgaacgc accggaagct gaggtggaag ctatttataa
    12421 agaagcagtg gaaaagcttc cgaaagaacc tgattttgac tgtgttatgg gggactggta
    12481 ctggagaaag gggcaatatg aaaaagcggt acagatgtat gaactggcta tagcaaagct
    12541 ggaaacctat gggacagtga acagggggat cattaccact tccatgctga tccagatgta
    12601 tgagtgtctg ggagaaggct gcagaaagct gggagattac cagaaagcgg tccgctattg
    12661 tgtgatcgta ctgaatactg accacaaaga tatgaatgcg ctgctgactc tgatcaactg
    12721 ttttacagag agcaatgtca gcgcagaaga ggtaatccag tttttaggga agatttatga
    12781 ttattctgat ataaaagaca aggtgatcct tctgcgggtg gctatggcaa tgggcagaaa
    12841 ggatctggag cggatgttca ggggaatcct ccttcctcag gagagggaag agtttgatgc
    12901 agccatggaa acggcccaga gcggggcgcc gctgtcatag acttttttca actcttccat
    12961 gagaaaagga agctcccatt cgcaggtgct gttatcaaag ctgttaggat ctgcgataga
    13021 aacatttccg tcaggaagga gttttgtgat caggacaaag tgaccatttt gcgtcaggct
    13081 tcccttaccc ataagggcga ccaggatatg accggaagaa agaagggaag ctgcgttttg
    13141 gggcgaatgg tctgttacag attctacatg aaagccatat gcagtaagga cagctgggat
    13201 cagactgtga taggagccgc tctggggggc ataatagcca ttggcagcag accagtctgc
    13261 gatagttacc ggggtaatgg cttctccgcc atttgtgaag ctggagatga gcatggcagc
    13321 agcagtagga ccacatccat actggagcat gggatctgaa ccgccgtaga gataactgct
    13381 ccagcgggta tctccctgat tatagtagta catcggtccc atactggtat ccaggatggc
    13441 agcatcataa gaattgccat ctgatcccgg ttttgcctcc atacccaggt tttctgcttt
    13501 tccaaggatc accggagaga cctttgacgt gacagaagat gtatctggga tcccatgtgt
    13561 cccggaagta tcctctgaaa gctctgtttg cttttggctt tttggctttg gctgagtctg
    13621 ctttttaaga aagtcttcca gttcatgaaa tttctggctg ctggcagtct gtacaaagga
    13681 aatgccggat cgtgcatctt ttatgacctg ggaagaaaag cctgggcaaa aataaaagct
    13741 caggctggca gtgatcaccg aaactgtacc ggtaatggaa agttggatca tcgttttcgc
    13801 aagttggaac tgtttcaagg ttttatttat atgttttttt tgattcttta aagttctatc
    13861 tttcattttt atccattcat ttcttatccg ttaacgaagg gatacacggg ccaggacagt
    13921 ctgaccaatg gtatttaaag gacactgctg gcatacagcg ccaatcttat gtgcctccat
    13981 aggcatgccg tagacaacac agctgttctt gtcctgtccg atggtatagg cgccgttttt
    14041 gcgcatacgc aaaagaccgg cagctccatc tgctcccatt cctgtgagaa gaacgccgat
    14101 ggcgtgttcc cgcacatgta atgcaactga gtcaaaaagc acatccacag aaggtctgtg
    14161 tccgtttact ttttctcctt cctgtacact gactgcatag gagtttccaa gaggtaccac
    14221 acgcatctgc agacctccgg gagctaaaag cataagacct ttgtggatcc ggtccccatt
    14281 tttcgcctcc cgtacttcca ttttgcagat cctgtttaag cgctctgcat acatagctgt
    14341 aaaacctgca ggcatatgct gggtgatcac aacacctggg gtgttttctg gaaaattctt
    14401 tactacttca agaatggcct ctgtacctcc ggtagatgcc ccgatggcga tcacggtttc
    14461 ctgggaagct gccgcagaag tctttggtct gttaagaaaa ggattacctg ccgcagttaa
    14521 aggagagact atccgttttt ttgctgcctg tggactggca ggaacggatg ggagatgtac
    14581 atgggaagca gcagccacca gaagcttgga acggagcata gaaagaaaga tctctttaga
    14641 cagtccattg gcttcatccg gtttccgcac aaaatccaca gctccggctt ccaaggcatc
    14701 aaaaaccctc aggttcaggg aagataccag gattaccggt atagggtgga ccggaagaag
    14761 ctgctttaaa aaatcaatgc cggacattcc cggcatttca atatccaggg taagcatatc
    14821 cggctgcagt agcgggatct tttcctgggc gtcaagggca tttactgcat atcctacaat
    14881 ctggaaacgg tcatccgcag agagagaacg gatcagccag gtgcggaaca agggtgaatc
    14941 atccaccacc attaatttta atttatgatc catgatcata atgcctcctt aataagataa
    15001 ctgtaacaac gtatgagata acctacggga tcagatcttt cggtaaatag ccggttcaat
    15061 ataggaaaac gggcagcttc ccttatccag gctttcagaa tgtccgataa ataagtagcc
    15121 tcccggagcg gtagcatcat aaaagcgcct taccagtgcc gccttcgtag gacggtcaaa
    15181 atagatcatc acgtttctgc agaagatcag gtcaaacttt ttgcggaaat ggatgggatc
    15241 catcaggtta aaggaacgaa agatcacatt tttccttaaa gcatctgaaa cagtataggt
    15301 tttatctcct atgggaacaa aatagcgttc ctgccaggaa ggcggaagtt cttttaagct
    15361 ttcaagggaa tatctgggct ccatggcaga atccagaaca cgctgggaaa tgtccgtagc
    15421 aaggatacgg gtatcccagc ttccgggaat agcggaaaaa tattctttca gatacataga
    15481 aatgttataa ggttcttcac cggaggagca gcccgcactc cagatagcaa tagaacgatc
    15541 catagaatgc ttgtttgcaa gctcgggaag aatatgcttc tggagaaaat caaaatgctc
    15601 cttttcccgc ataaagtagg tataattggt agtcagcttg ttcaacataa cagaaaccat
    15661 cgccggatct ttgccggaga tgatctcatc tacgtattct gtaaaactat ggaatccttt
    15721 ctgctgcagc atattagtaa gacggctggt gatcagctgt tttttgtggc tcaggttgat
    15781 accatagcgc tgctggatat aggtataaag gcggttaaaa tccccgtcac ttaatgtaag
    15841 cattgtatga cctccttagg aattggatct actccagaag tctttccagc accagctttt
    15901 gtggttgacg aaaaatttta tcacaaaatc aggtacagaa agattgtggg agaacattaa
    15961 ggactgacca gggacttgca gtcaaaagac atgagcacag taccattttc aagtgtgatc
    16021 ataccatcta acagcggttt ccccttgtat cgtcaccgta taataccctt tttgttccgc
    16081 attcgtattt tccggtaagc taggatcata atcaaagctc accaacaa
//
LOCUS       DBMDUA010000048        15913 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2072_length_15913_cov_3.863838, whole genome shotgun sequence.
ACCESSION   DBMDUA010000048 DBMDUA010000000
VERSION     DBMDUA010000048.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 15913)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15913)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15913
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2072_length_15913_cov_3.863838"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="ACZPCL_05545"
     CDS             complement(<1..1350)
                     /locus_tag="ACZPCL_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HIR1161973.1"
                     /translation="MAEKNKKTFKMPHTFVIMMVIILFATLLTWIIPAGQYVRVENAD
                     GVKVVDPNQFSFIDRCPVNPLLIPLYIVNSMCKRVDLMLVIIFSGGAFDLISKSGALH
                     SAVARVAKVFQNRLYIFIPIMTTIFALICTTQGVNQFIAFAPVVVMITLAMGLDSIVG
                     AAIILLGGAVGFATGTVNPSTTVVAQKIAELPLFSGIQYRAVCFVVFLIITNIYLVRY
                     ALKIRKNPELSPMYDLDSQNEQKDLDIDSFGTMDVRKTLIIIALVAALAGMVVGSVKF
                     GWDMPELAAVFVGLGIVVGFIAGETPSNMSKVFVEGCKKMLTAAMIIGLASAIASIMS
                     AGKIVDTVVYGLASCLQHTPGFLMAPAMYIANTLINCVIVSGSGQAAAIMPIMIPLSD
                     LLGVTRQTSVLAYNFGDGFCNYILPHSTALMGIISAVNIPYDRWLKFMWKLFLIWAVT
                     "
     gene            complement(1460..2428)
                     /locus_tag="ACZPCL_05550"
     CDS             complement(1460..2428)
                     /locus_tag="ACZPCL_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1161974.1"
                     /translation="MNLKEQEYVCALAENGTITAAAKELFISQPALSIYINNLEKSLG
                     VRLFERVGKQFILTYAGEQYVEKAKLILQLSHELDEKLKDITGNYSGRIRIGVQLRRA
                     TGFLPPVIASYEKEFPGVEVILCEGTMKELKESIQNFKADLLLCNSVDLPPYMNSFVV
                     FKEHLLIAVPKDHPINEKAVWEEGKALPSLELFWLNGEKLILAKQNQSIRRDSERILN
                     KNGVRPGKIREIRSIETAMQMVGEGLGIGFNRESYVMYMKKQENVRYYCMRNIDSTTD
                     FVLAYRKEMPVTGYMQRMIDMLMEHGKECEKIFPQVIRQHGCQNAF"
     gene            2580..2690
                     /locus_tag="ACZPCL_05555"
     CDS             2580..2690
                     /locus_tag="ACZPCL_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024945.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1161975.1"
                     /translation="MTLSQYIRCNYNKNETARELNLHRQSLIYRLKKLKN"
     gene            complement(2735..4438)
                     /locus_tag="ACZPCL_05560"
     CDS             complement(2735..4438)
                     /locus_tag="ACZPCL_05560"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HIR1161976.1"
                     /translation="MSDQKQTKENETMNAAQVLVRCLENEGVEYVFGIPGEENLEVMR
                     ALKDSKIKFITVRHEQGAAFMADVYGKLKGKAGVCLATLGPGATNLVTGVADANTDGA
                     PLIAITGQVGTERMHLTSHQYLDLVTMFTPITKRSKQVVNPDTVNEVVRIAFKYAESE
                     KPGACHIDLPCNIAAMPVESEVGRQPLKHHKPNLEMASVESLEEAAGLLFQAKRPVIL
                     AGHSAIRNKASKALTEFAEKLHIPVINTMMAKGIIPCDNLYSLWTIGIPQKDYQNIAI
                     EEADLVIAVGYDIVEYAPNKWNSKGNHTILHIDARPCHINKLYQPDVEVIGDLSYSLN
                     HIAMRCHRNDKPEWAFKLRDEMRKEHEAYAKDMEFPMKPQKILNDVRKVMGKEDIVIS
                     DVGAHKMWIARHYNCYEPNTCIISNGFATMGIAVPGALAAKLINPDKKVLAITGDGGF
                     MMNCQEFETALRTGTPFVTLVFNDCSYGLIKWKEEDRYGENFYVDFTNPDFVKFAESM
                     HAIGYRINSAEELIPTLEKAFEQKVPVIIDCPVDYRENTKLTEHLKELINSYQAQQPE
                     A"
     gene            complement(4683..6479)
                     /gene="aspS"
                     /locus_tag="ACZPCL_05565"
     CDS             complement(4683..6479)
                     /gene="aspS"
                     /locus_tag="ACZPCL_05565"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR1161977.1"
                     /translation="MAESMVGLKRSHRCTEVTSAQIGEKVTVMGWVQKSRNKGGIIFV
                     DLRDRSGILQIIFEENDCGTENFEKAEKLRSEFVIAVEGRVEARSGAVNENLATGAIE
                     VRAESLRILAESETPPFPIEEKSKTKEDLRLEYRFLDLRRPDIQRNLMLRSRVATLTR
                     AFLAEEGFLEIETPTLIKSTPEGARDYLVPSRVHPGSFYALPQSPQLFKQLLMVSGYD
                     RYFQLARCYRDEDLRADRQPEFTQIDMELSFVDVDDVLDVNERLLKKLFKEVCNYDLQ
                     LPIQRMTWREAMDRFGSDKPDLRFGMELKNVSEVVKDCEFVVFKSALENGGSVRGINA
                     EGQGHMPRKKIDALVEYAKGFGAKGLAYIAINEDGTYKSSFAKFMKDEEMAALVAAMD
                     GKPGDLLLFAADRDKVVFDVLGNLRLELARQLDLLKKDDFKFLWVTEFPLLEYSEEEG
                     RFVAMHHPFTMPMDEDLQYIDSDPGRVRAKAYDIVLNGVEMGGGSVRIHQADIQSKMF
                     EVLGFTPERANDQFGFLLKAFKYGVPPHAGLAYGFDRMIMLMVGADSIRDVIAFPKVK
                     DASCLMTKAPTPVDPKQLEDLGIEVIPEEKEE"
     gene            complement(6537..7802)
                     /gene="hisS"
                     /locus_tag="ACZPCL_05570"
     CDS             complement(6537..7802)
                     /gene="hisS"
                     /locus_tag="ACZPCL_05570"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR1161978.1"
                     /translation="MALTKKPVTGMKDITPAEMQIREYVMNQIRETYKGFGFSQIETP
                     CVEHIENLTSKQGGDNEKLIFKILKRGDKLNLETAKEENDLADSGLRYDLTLPLSRYY
                     ANNAANLPSPFKALQMGSVWRADRPQKGRFRQFTQCDIDILGDPTNLAEIELILATTT
                     ALSKICPDNAFTVRINDRGILFGMARYCGFAEEAMDSVLITLDKMDKIGFEGVEKELL
                     ENGNAPESVSRYMEILRTVTNDAEGVKKLGETLKDVMDPSVCQGLVHIMETVKDVAEA
                     EFGLVFDPTLVRGMSYYTGTIFEVSMEGFGGSVAGGGRYDKMIGKFTGMETPACGFSI
                     GFERIVTILLDNGFTVPGAGEKKAFLFEKGIDSASLAAVIREAMEERKKGVRVLVAQM
                     NKNKKFQKEQLGKEGYQEFKEFYKESLKH"
     gene            complement(7886..9346)
                     /gene="hemZ"
                     /locus_tag="ACZPCL_05575"
     CDS             complement(7886..9346)
                     /gene="hemZ"
                     /locus_tag="ACZPCL_05575"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HIR1161979.1"
                     /translation="MIGLLIQDNAYEQDIRELLMSFYPGETYAHEVKEGVDFYVQTRL
                     EDGEAVIGIWEKLENGEELEGWTLTAHSTGKADLSDHSATKNVIKKLFYQMLVKRTGH
                     ELPWGSLTGIRPTKIALSRLEDGWKEEDIRKFMKETYMTSDEKINLSIEIAAREKRLL
                     EPLDYDRGYSLYVGIPFCPTACLYCSFTSYPISKWKGRTGLYLEALFKELEYTAEKMK
                     GRPLDTIYFGGGTPTSLPAEDIDAILCKLEQLFDTKNALEFTVEAGRPDSITEEKLKV
                     LASHGISRISINPQTMNQKTLDLIGRRHTVENVKEKFHIARELGFDNINMDLIMGLPG
                     EDLDDVKHTLEEIEALKPDSLTVHSLAIKRAARLNMFKEEYADLKINNTPEMIALSEA
                     CARRMGMEPYYLYRQKNMAGNFENVGYSLPGKACIYNILIMEEMQTIAACGAGTTTKV
                     VFPSENRRERCENVKEVEQYISRIDEMIGRKEKIIH"
     gene            complement(9385..10026)
                     /locus_tag="ACZPCL_05580"
     CDS             complement(9385..10026)
                     /locus_tag="ACZPCL_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1161980.1"
                     /translation="MSNFRIKTMVLGMVSTNCYLVYNDDTKEGVVVDPADNGAYILNK
                     CSELGVTPVAVLLTHGHFDHIIGVPEVKRAFHIPVYASETEDAMLADTSINLSCHVPG
                     KQTSFHADVLVHDGDELELLGYKWKVIATPGHTAGSVSYYIPEEEVLLSGDTLFYESY
                     GRTDLATGSSSDIADSLLNKLFVLPEDTMVYPGHGDPTTIGYEKEHNPIPYYR"
     gene            complement(10087..12372)
                     /locus_tag="ACZPCL_05585"
     CDS             complement(10087..12372)
                     /locus_tag="ACZPCL_05585"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR1161981.1"
                     /translation="MGKEGTKEKLDIELEAPADFTSPEVLYQDLVKTIHKYHPSDDIS
                     MIEKAYKVAAEAHKDQKRKSGEPYIIHPLCVAIILADLEMDKETIAAGLLHDVVEDTV
                     MTLDELTKEFGPEVAFLVDGVTKLTQLNWDKDKVEIQAENLRKMFLAMAKDIRVIIVK
                     LADRLHNMRTGQYWKPEKQKEKARETMEIYAPIADRLGISKIKIELDDLSLKFLKPEV
                     YYDLVEKVDLRKDAREAFVQSIVDEVKAHLDEAGIEATVGGRVKHFFSIYKKMLKQNK
                     TLDQIYDLFAVRIMVDTVKDCYAALGVIHEMYKPIPGRFKDYIAMPKQNMYQSLHTTL
                     IGSTGQPFEIQIRTYEMHRTAEYGIAAHWKYKESGSGQIAAGKEEEKLSWLRQILEWQ
                     RDTDDSKEFLSMVKGDLDLFSDSVYCFTPSGDVKTLPSGSTPIDFAYSIHSAVGNKMV
                     GARVNGKLVPIEYQIQNGDRIEIITSQNSKGPSRDWLKLVRSTQAKNKINQWFKTQMK
                     EDNIIRGRDAIDRYCKAKGINLADLSKPEFMEKVMNRYAFKDWDSVLASIGHGGLKEG
                     QVINKMLEEREKKLKREITDAEVLDGIADTDGRSGEATVRKNKSGIVVKGLHDLAVRF
                     SRCCNPVPGDEIVGFVTRGRGVSIHRTDCINVINLPEDERGRLIDAEWQVAENPTSTE
                     QYSTEIQIFANNRIGLFADISKVFTEKQIDITSMNVRTSKQGRATIIMTFDIHGTEEL
                     NRITDKIRQIDGVLDIQRTTG"
     gene            complement(12473..12997)
                     /locus_tag="ACZPCL_05590"
     CDS             complement(12473..12997)
                     /locus_tag="ACZPCL_05590"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIR1161982.1"
                     /translation="MKKLEEYVRSIPDFPEPGIIFRDVTTILQDADGLHLAVDSIRNM
                     LKDVDYDVVVGPESRGFIFGVPVAYAEHKGFVPVRKKGKLPCETVSAKYALEYGTAEI
                     EMHKDSITPGQKVVIIDDLIATGGTTEAIIKLIEELGGKVVKICFVMELAGLKGRELL
                     KGYDVESAIIYEGK"
     gene            13406..14128
                     /locus_tag="ACZPCL_05595"
     CDS             13406..14128
                     /locus_tag="ACZPCL_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HIR1161983.1"
                     /translation="MTGLGTLVNMAAILIGSGLGLLLKNGIPKRLQTTISSAVGLCLI
                     FVGITGAIKGLMNVSGTTFETKDTLVVVICMAIGAAIGECLDLEQRMENLGDWVKSKL
                     PNGSSSSNFTEAFVSTSLVFCIGAMAIVGSIEDGLNHDYTMLFTKAVMDGVLSIVFVA
                     SLGIGAAFSMFPVGIYQGSITLLAGLVKPYLTDLMIGRLSCIGSILIFALGLNLTIGT
                     KIKIANMLPAVFLPVIVCLLGF"
     gene            14177..14926
                     /locus_tag="ACZPCL_05600"
     CDS             14177..14926
                     /locus_tag="ACZPCL_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1161984.1"
                     /translation="MLKEERQTWILNKLHSSGKVVVSHLAGELKVSEDTIRRDLLDLD
                     QKGQLKRVFGGAIPLEKPVINFFDRETADVALKQQLAMRALEFLEPDQLVAIDGSSTN
                     LQFAKNIPDNLKLTVLTNSYSIAHVCSLKEQIRVIILGGDLLKDSMTTVGEVAAAQAA
                     AYHPDLCFMGVYAIHPEYGMTIPYPEEVSIKRQLIRSSSRVIALVNPIKLNTVSRYQV
                     CGIEDFTTLITDGNVPQEMASRYKNRGLDFL"
     gene            complement(15154..15807)
                     /gene="fsa"
                     /locus_tag="ACZPCL_05605"
     CDS             complement(15154..15807)
                     /gene="fsa"
                     /locus_tag="ACZPCL_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIR1161985.1"
                     /translation="MKFFIDTANVDDIRKANDMGIICGVTTNPSLIAKEGRDFKEVIK
                     EITSIVDGPISGEVKATTVTAEGMIEEGREIAKIHPNMVVKIPMTVEGLKAVKVLHAE
                     GIKTNVTLIFSAAQALLAARAGATYVSPFLGRLDDISMPGIDLIYDITEIFQMHNIET
                     EIIAASVRNPIHVIDCAKAGADIATVPYKVLEQMTKHPLTDQGIAKFQADYKAVFGE"
BASE COUNT         4063 a         3884 c         3194 g         4772 t
ORIGIN      
        1 tgtaactgcc cagatcagga acagtttcca cataaacttc agccaacggt catatgggat
       61 gttaacagca ctgatgatac ccataagagc agtagaatga ggcagaatat agttacagaa
      121 accatcaccg aaattatatg ccagaacact ggtctgacgg gtaacgccta acaggtcaga
      181 taatgggatc atgatcggca tgatcgctgc cgcctgtcca ctgccagata cgatcacaca
      241 gttgatcagt gtatttgcaa tatacattgc aggagccatt aagaagcccg gtgtatgctg
      301 taagcaggaa gctaaaccat atacaacagt atctacgatc tttcctgcac tcatgatact
      361 tgcaattgca cttgcaaggc cgatgatcat agcagcagtc agcatcttct tacagccttc
      421 aacaaatacc ttagacatat tagaaggagt ttctcctgca ataaatccaa ctacaatacc
      481 aagtcctacg aatacagctg ccagctccgg catatcccag ccaaacttaa cagaaccaac
      541 taccataccg gctaaagctg caaccagagc gatgatgatc agggtttttc ttacatccat
      601 ggttccgaaa gagtcaatat ctaggtcttt ctgctcattc tggctgtcca ggtcatacat
      661 tgggcttaac tctggattct tgcggatctt taaagcatat cttaccagat aaatgtttgt
      721 aataataagg aataccacga agcagactgc acggtactgg atccctgaaa acagtggcag
      781 ttctgcgatc ttctgggcaa caacagtggt acttggattt acagtaccgg ttgcaaaacc
      841 aactgcgccg cctaacagga tgatcgccgc accaacaatg gagtcaagtc ccattgccag
      901 agtgatcatt acaactacag gagcaaatgc gatgaactgg tttacaccct gagtggtaca
      961 gatcaacgca aagatggtag tcatgatcgg gataaagata tacagacggt tctggaatac
     1021 ctttgcaaca cgggctactg cagaatgaag agcgccggat ttgctgatca ggtcaaacgc
     1081 accgcctgag aaaatgatga ctaacatcag atccactctc ttacacatgg aatttacaat
     1141 gtaaagaggg atcagaagtg ggttaactgg acagcggtca ataaaggaga actggttcgg
     1201 gtctactacc tttactccgt ctgcgttttc cacacgcaca tactgtcctg ccggaatgat
     1261 ccaggtcagt aaggttgcga acaggatgat gaccatcatg atcacaaatg tgtgaggcat
     1321 tttaaatgtc tttttgtttt tttctgccat tttttaatac tccttccgta cttttttatt
     1381 attttatcac actaaagcgt agaagtacat ttaattttat ttatttttta tataaattta
     1441 ataaataata atgtttgcat caaaaggcat tttgacatcc atgctgccgg atcacctgcg
     1501 gaaaaatctt ctcacattct tttccatgct ccataagcat atcgatcata cgctgcatat
     1561 agccggtcac cggcatttct ttccggtatg ccagcacaaa atcagtggtg ctgtcaatat
     1621 tacgcataca ataataccgc acattttcct gctttttcat atacatcaca tagctttccc
     1681 ggttaaatcc gatccccagt ccttctccta ccatctgcat ggcagtttct atgctgcgta
     1741 tctcccgtat ttttcccggc cttactccat ttttatttaa tatacgttct gaatccctgc
     1801 ggatactctg gttctgtttt gccagtataa gcttttcccc gttcagccag aaaagctcca
     1861 gacttggaag ggcttttcct tcttcccaga ctgccttttc attgatggga tgatcttttg
     1921 ggacagcaat aagaagatgt tctttaaata ccacaaaact attcatatag ggaggcaggt
     1981 ctacactgtt gcataaaagc agatctgctt taaaattctg tatactttct tttaattctt
     2041 tcatggttcc ctcacaaagg atcacttcca ctcccggaaa ctccttttcg taagaggcaa
     2101 taacaggggg caaaaatccg gttgcccggc gcagctggac cccgatgcgg atgcgtccgc
     2161 tgtaatttcc agtaatatcc tttaattttt catccagttc atggctcagc tgtaaaataa
     2221 gcttcgcctt ttccacatac tgctctcctg cataagtaag gataaactgc tttcccaccc
     2281 gctcaaataa ccggactccc agggattttt ccagattgtt aatgtatatg cttaatgctg
     2341 gctgggaaat aaaaagctcc tttgccgctg ctgtaatcgt tccgttttct gccagtgcac
     2401 agacgtattc ctgttccttc agattcatga tcatgcctcc tgtgtgctga gactttttta
     2461 tcttctgtca tgtactagta catcgtttca tatattatac ttaattatcc gaaagaatgg
     2521 caagatttat tgctttaaaa agcataatga tcaaaagtat ttctgtctag gaactgctgc
     2581 tgactttatc ccaatacatc cgttgtaatt acaacaaaaa cgagactgcc cgggagttaa
     2641 atctccaccg gcagtccctt atataccgtt taaaaaaatt gaagaactaa caggtcttag
     2701 tttaaatgac catgattctt tatatcttct ggaatcatgc ctcaggctgc tgcgcctgat
     2761 atgagtttat taattccttt aaatgctccg taagcttcgt attctcccga taatccaccg
     2821 ggcagtcaat gataacaggt accttctgtt caaatgcttt ttccagagtc gggatcagtt
     2881 cttcggcact gttgatccga tatccgatgg catgcatact ttctgcgaat tttacaaaat
     2941 ccgggttggt aaagtctaca tagaagttct ctccataccg gtcttcttct ttccatttga
     3001 tcaggccata gctgcaatca ttaaagacca gggttacaaa aggagttccg gtgcgcaggg
     3061 cagtctcaaa ttcctggcag ttcatcataa aaccgccatc accagtgatc gccagtacct
     3121 ttttatccgg gttgatcagc ttggctgcca atgcacccgg aacagcaatt cccatggtgg
     3181 caaagccatt ggaaataata caggtatttg gctcataaca gttataatgc cttgcgatcc
     3241 acattttatg ggcacctaca tcagaaataa cgatatcttc ttttcccatt accttgcgca
     3301 catcatttaa gattttctgg ggcttcatag gaaattccat atctttggca taagcctcat
     3361 gctccttacg catttcatca cgaagcttaa atgcccactc aggtttatca tttctatgac
     3421 aacgcatggc aatatgattt aaagaatagg acaaatctcc gatgacctct acatccggct
     3481 gatacagctt gttaatgtgg catggtcttg cgtcaatatg gagaatggtg tgatttccct
     3541 tgctgttcca tttattaggt gcatattcta caatatcata gcctactgcg atcaccagat
     3601 ctgcctcttc aatagcaata ttctggtagt ctttctgtgg aataccgata gtccataaag
     3661 aatataggtt atcgcagggg ataatgccct ttgccatcat agtattgatc accggaatat
     3721 gcagtttctc cgcaaattcc gtcaaagcct tagatgcttt attccggata gcagaatgtc
     3781 ctgcaaggat cactggacgt tttgcctgga aaagcagtcc tgctgcctct tccagacttt
     3841 ctacacttgc catttccaga tttggcttgt gatgctttaa aggctggcgt cccacttcac
     3901 tttctacagg catagctgca atattacagg gcaggtcaat gtggcaggcg cctggtttct
     3961 cactttctgc gtatttaaag gcaatgcgga ctacctcgtt tacggtatcc ggatttacca
     4021 cctgtttgct gcgctttgtg atcggtgtaa acatggttac aagatccaga tactggtggg
     4081 atgtaaggtg catacgctcc gttcctacct gaccggtaat ggcgatcagc ggtgcgccgt
     4141 ctgtattggc atctgccaca cctgttacca ggttggtagc accagggcct aaggttgcca
     4201 ggcacacacc agctttgcct tttaatttac cgtaaacatc tgccataaaa gctgcgccct
     4261 gctcatggcg gactgtgata aatttgatct tagagtcctt taacgcccgc attacctcca
     4321 gattttcttc tcccggaata ccgaaaacat attctactcc ttcattttcc aggcatctta
     4381 ctaacacctg tgctgcattc attgtttcat tttcttttgt ctgtttctga tcgctcatga
     4441 tctgctgcct ccctttgatc tgatcctgat ttgttttcca taatatatgg accccacaga
     4501 acagaaaaag cccccgaaga tctaactctt cagaggcttc tttgccgttt catgttttct
     4561 ggattctttt tctgaaacgg attatagcat cccaaaactt ctttttccaa cacgaaaatc
     4621 attgttccgg gtacagcatt tttctttaat tataatccat gagttttatg gacacaaact
     4681 ttttactctt ctttttcttc cggaattact tcaattccca gatcctctaa ctgctttgga
     4741 tctacaggag ttggtgcctt tgtcataaga caggaggcat cttttacctt cgggaaggca
     4801 attacgtcac ggatgctgtc tgcgcctacc attaacatga tcatacggtc gaaaccatat
     4861 gccagacctg cgtgaggtgg tactccgtac ttaaatgcct ttaacaggaa gccaaactgg
     4921 tcatttgctc tttctggtgt aaagccaagt acttcaaaca tcttagactg gatatctgcc
     4981 tggtggatac ggacagaacc accgcccatt tctacgccgt ttaatacgat atcgtaagcc
     5041 tttgcacgta ctcttcccgg atcggagtca atgtactgaa ggtcttcatc cataggcatg
     5101 gtaaatggat gatgcattgc tacgaaacgt ccttcttctt cggagtactc taataacggg
     5161 aactctgtta cccagaggaa cttaaagtca tctttcttta acaggtctaa ctgtcttgcc
     5221 agttccagac gcaggttgcc aagtacatca aatactacct tgtccctgtc agctgcaaag
     5281 agtaataagt cacctggctt accatccata gcagcaacta aagcagccat ttcctcatcc
     5341 ttcatgaatt tagcgaagga agacttgtag gtgccatctt cattaatagc aatataagca
     5401 aggccctttg cgccgaagcc ctttgcgtac tctactaagg catcgatctt ctttcttggc
     5461 atatgtccct gtccctctgc gttgataccg cggacagaac caccattctc taaagcgctc
     5521 ttaaatacaa cgaactcgca gtccttaaca acttcagaaa cgttctttaa ttccattccg
     5581 aaacgaaggt ctggcttatc agaaccaaaa cggtccatag cttctctcca ggtcatacgc
     5641 tggatcggca gctgcagatc atagttgcag acttctttga acagcttctt taacagacgc
     5701 tcatttacgt ctaaaacgtc atctacgtct acgaaagaaa gctccatatc gatctgggta
     5761 aactcaggct gacggtctgc acgaaggtcc tcatcacggt agcagcgtgc taactggaag
     5821 taacggtcat aaccggaaac catcagtaac tgcttaaaca gctgtggaga ctgtggcagt
     5881 gcgtagaaag aacctggatg tacacggctt ggtaccaggt aatctcttgc gccttctggt
     5941 gtactcttga tcagagttgg ggtttcgatc tctaaaaatc cttcttctgc caggaatgca
     6001 cgggtcagag tagcaacgcg gctgcgcagc atcaggtttc tctggatgtc aggacggcga
     6061 agatccagga aacggtattc cagacgcaga tcttcttttg tcttgctctt ttcttcaatt
     6121 gggaaaggag gagtctcaga ctcagccagg atacgtaagg actctgctct tacttcgatg
     6181 gcacctgtag ccaggttttc atttacagcg ccggaacgtg cctctacacg gccttctact
     6241 gcaataacga actcacttct taatttctca gctttctcaa agttttcggt tccgcagtcg
     6301 ttttcctcga aaatgatctg aaggatacca gaacggtctc ttaaatcaac gaaaataatt
     6361 ccgcctttgt ttctgctctt ctgaacccag cccataacgg ttactttctc gccgatctgg
     6421 gcagaagtta cttctgtaca tctgtgggac cgcttcagtc ctaccattga ttctgccatg
     6481 atttttctcc tttatagcta ttaactaata aattacccta ctgtatataa gtacgattaa
     6541 tgtttcaggc tttctttata gaattcctta aactcctgat agccttcttt tccaagctgt
     6601 tccttctgga acttcttgtt cttgttcatc tgggctacca gtacgcgaac gcctttcttt
     6661 ctctcttcca ttgcttcacg gatcacagct gctaaagaag cggagtcaat tcccttttca
     6721 aagaggaatg ctttcttctc accagcacca ggaaccgtaa atccattgtc taagagaatg
     6781 gtaacgatac gctcaaaacc aatggagaag ccgcatgcag gtgtctccat accggtaaac
     6841 ttgccgatca tcttgtcata tcttccgccg cctgctacag aaccgccgaa gccttccatg
     6901 gaaacctcga aaatagttcc tgtatagtag gacattccac gtaccagagt cgggtcaaat
     6961 acaaggccaa attcagcctc tgctacgtct tttacagtct ccataatgtg taccagtccc
     7021 tggcatacag atggatccat tacatccttt aaagtctctc ctaacttctt aacaccttct
     7081 gcatcattgg taacagtgcg aaggatctcc atataacggc ttacgctttc aggagcgttt
     7141 ccattttcca gaagttcttt ctcaacaccc tcaaaaccga tcttgtccat cttgtccaga
     7201 gtgatcagaa cgctgtccat agcttcttct gcaaaaccgc agtaacgtgc catgccaaaa
     7261 aggatgcctc tatcgttaat tcttacagta aatgcattgt ccgggcagat cttgcttaat
     7321 gcagtagtag ttgccaggat cagctcgatc tcagccagat tggtaggatc gcctaaaata
     7381 tcaatatcgc actgggtaaa ctgacggaaa cggcccttct ggggacggtc tgctctccat
     7441 acgcttccca tctgtaatgc cttaaaagga cttggaaggt ttgccgcatt atttgcataa
     7501 taacgggaca gaggcagtgt caggtcataa cgaagaccgc tgtctgccag gtcattttct
     7561 tcctttgctg tctctaaatt cagcttatcg cctctcttta agatcttaaa gatcagcttt
     7621 tcattatcac cgccctgctt gctggtcagg ttctcaatat gctccacgca aggagtttcg
     7681 atctgtgaaa aaccaaagcc cttgtaggtc tcacggatct ggttcataac atattcgcgg
     7741 atctgcatct ctgcaggagt gatatctttc atgccggtaa ccggcttctt tgttaatgcc
     7801 atatttttct ccctgttttc tatctgttct ctatctgtac tattcctttt ttattgtaat
     7861 gaactttccc gattttgcaa gtcctttagt gaataatctt ctccttgcgc ccgatcattt
     7921 catcgatccg gctgatatat tgctctactt cctttacatt ctcgcagcgt tcccttctgt
     7981 tttcagatgg gaatactact tttgtagtag ttcctgcacc gcaggcagca atggtctgca
     8041 tctcctccat gatcaggata ttgtagatac atgcctttcc tggaagagaa taacctacat
     8101 tttcaaagtt gcctgccatg tttttctggc ggtagagata atatggttcc atacccattc
     8161 tgcgggcaca ggcttcactt aaagcaatca tttccggtgt gttgttgatc tttaagtccg
     8221 catattcttc cttaaacatg ttcagtcttg cagcacgttt aatagcaaga gaatgaacgg
     8281 tcagagagtc tggttttaag gcttcgatct cttctaatgt atgttttaca tcatccagat
     8341 cttcccccgg aagtcccatg atcaggtcca tattgatgtt gtcaaagcca agttctcttg
     8401 caatgtggaa cttttctttt acattttcta ctgtatgacg gcggccgatg agatccaggg
     8461 tcttctggtt catggtctgt gggttaatag aaatacggct gatgccatgg gaagctaaaa
     8521 cctttaactt ttcttctgta atgctgtccg gacgtcctgc ttctacggtg aattccagcg
     8581 cattctttgt atcaaacagc tgctctaatt tacataagat agcgtcaatg tcctctgcag
     8641 gcagggaagt cggtgtaccg cctccaaaat aaatggtatc aagaggtctt cccttcatct
     8701 tttccgctgt atattccagt tccttaaata aggcttccag atacaatccg gtacggcctt
     8761 tccacttact gataggataa gaagtgaagg agcagtacag gcaggcagtc gggcagaatg
     8821 ggatacctac atataagctg taacctctgt catagtccaa cggctctaac agacgttttt
     8881 cccttgctgc gatctcaatg ctcagattta tcttctcatc gctggtcatg taagtttctt
     8941 tcatgaattt gcggatgtct tcttccttcc atccatcttc cagacggctt aatgcgatct
     9001 tggtaggacg gatccctgta agagaacccc atggaagttc atggccagta cgctttacaa
     9061 gcatctggta gaataatttt ttaataacgt tttttgtggc agagtggtct gagagatctg
     9121 cttttcctgt ggaatgggca gtcagagtcc agccttccag ttcttcccca ttttctagtt
     9181 tttcccagat cccaataaca gcttccccgt cctctaatct ggtctgtaca taaaaatcaa
     9241 cgccctcttt tacttcatgg gcataagtct cacccggata aaaagacatc agaagttctc
     9301 tgatgtcctg ttcatatgca ttatcctgta ttaataatcc gatcattttc ttcctgcttt
     9361 ctatctatcg tacctctttc gtacttatct gtaatacgga ataggattgt gctccttttc
     9421 atagccaatg gtagttggat ccccatgtcc cggatagacc atagtatctt ccggaagcac
     9481 aaataattta tttaaaaggg aatccgctat atcactgctg cttcctgttg ccaggtctgt
     9541 acggccatag gactcataaa acagcgtatc accggaaaga agtacctctt cttccggaat
     9601 atagtagctg acggaacctg ctgtatgtcc aggtgttgca atgactttcc atttatatcc
     9661 taaaagttct aattcatccc cgtcatggac cagtacatct gcatggaaac tggtctgttt
     9721 tccaggcaca tggcaggaca gattaataga agtatctgcc agcatggcat cttctgtttc
     9781 agaggcatat acaggaatgt ggaatgcccg ctttacctca ggcactccga taatatgatc
     9841 aaaatgtcca tgtgttaaaa gcactgccac cggtgttaca cccagttcac tgcatttatt
     9901 taaaatatag gctccattat ctgccgggtc taccactacg ccttctttgg tatcgtcgtt
     9961 gtagaccaga tagcagtttg tgctcaccat tccaagcacc atggtcttta tcctgaaatt
    10021 actcatagtt ctccttctat atggcactgt ctgccactgc agatccggcc ggatgcagca
    10081 gtttcatcag cctgtagttc tctggatatc cagaacaccg tcgatctggc ggatcttatc
    10141 ggtaatacgg ttcagttctt ctgttccatg gatatcaaag gtcataatga tggtggcgcg
    10201 gccctgcttg ctggtacgca cgttcatgga agtaatatcg atctgctttt ctgtaaatac
    10261 ctttgaaata tctgcaaaaa ggccgatacg gttgtttgcg aagatctgga tctcagtgga
    10321 atactgctct gtactggtag gattttctgc tacctgccac tcagcatcga tcagccgtcc
    10381 tctttcatct tccggaaggt tgatcacatt gatacagtct gtacggtgga tggaaacgcc
    10441 tcttcctctg gtaacaaaac caacgatctc atcaccaggt acaggattac aacatctgga
    10501 gaaacgaact gccagatcat gaagtccctt gaccacgata ccgctcttat tcttgcgtac
    10561 agttgcttcg ccgcttcttc catctgtatc ggcaatgccg tccagaactt ctgcgtcagt
    10621 gatctcacgt ttcagcttct tctcccgctc ttccaacatc ttgttaatga cctggccctc
    10681 ttttaagcca ccatgtccaa tagatgcaag aacagaatcc cagtctttaa aggcatagcg
    10741 gttcattacc ttttccataa attccggttt ggaaaggtcc gccagattaa tgccttttgc
    10801 cttacaataa cggtcaatgg catcacggcc gcggatgata ttatcctctt tcatctgggt
    10861 cttgaaccac tgattgatct tattttttgc ctgtgtgctg cgtaccagct tcagccagtc
    10921 gcggcttggt cccttggaat tctgggaggt gatgatctca atacggtcac cattctggat
    10981 ctgatattca ataggaacca gttttccatt gactctggcg cctaccatct tatttcctac
    11041 cgcactgtgg atggaataag caaaatcaat aggagtagag ccgcttggaa gagtctttac
    11101 atcaccggac ggagtgaaac agtaaacact gtcagagaac aggtccagat caccctttac
    11161 catgcttaaa aactctttgg aatcatctgt atcacgctgc cactccagga tctggcgcag
    11221 ccagcttaat ttctcttctt cttttccggc agcgatctgt ccgcttccgc tctctttgta
    11281 tttccagtga gcagcgatac catactcagc agtacggtgc atctcgtaag tacggatctg
    11341 gatctcaaat ggctgtccgg tgctgccgat cagagttgta tgcagagact ggtacatgtt
    11401 ctgcttcggc atagcgatat agtctttaaa acgaccagga attggcttgt acatttcatg
    11461 gatcacacca agagctgcat aacagtcttt tactgtgtct accatgatac gcacagcaaa
    11521 cagatcgtag atctgatcca gggtcttgtt ctgctttaac atcttcttat aaatactgaa
    11581 gaaatgcttt actctgccgc ctacggtggc ttcaatgcct gcttcgtcta aatgtgcctt
    11641 tacttcatct acaatggact ggacaaaagc ttctcttgca tctttgcgca gatccacttt
    11701 ttctaccagg tcataataaa cttccggctt taaaaactta agtgataagt catctaattc
    11761 gatcttaatc ttggaaatac ccagacggtc tgcaatagga gcatagatct ccatggtctc
    11821 ccttgctttt tccttctgct tctccggctt ccagtactgg ccggtacgca tgttgtgaag
    11881 acggtctgcc agcttaacga tgataacgcg gatatccttt gccattgcaa ggaacatctt
    11941 gcgcaggttc tcagcctgga tctccacttt atctttatcc cagtttaact gagtcagctt
    12001 ggtaacaccg tctaccagga aagcaacctc gggaccaaac tctttggtca gctcatccag
    12061 tgtcatgacg gtatcttcta ctacgtcatg aagaaggcca gctgcaatag tctccttgtc
    12121 catctccaga tcagccagga taatggccac acataaagga tggatgatat aaggctctcc
    12181 agactttctc ttctgatcct tatgtgcttc tgcagcgacc ttgtatgcct tttcgatcat
    12241 ggaaatatca tcggaaggat ggtatttatg gatcgttttg accagatcct gatacaacac
    12301 ttccgggctg gtaaagtccg ccggagcttc aagctctata tccagttttt cttttgttcc
    12361 ctcttttccc atatatgtgg ccaccttttt acaaataatt agttaccatt ataatttttt
    12421 ttaaataaaa aagcaaggtc tggtatacct attactttct tatgagttct gattacttac
    12481 cctcatagat aatagcagac tcaacatcat agccctttaa aagttctctt cccttaagtc
    12541 ctgccagttc cattacaaaa cagatcttca ctaccttgcc gcccagttct tcgatcagct
    12601 tgataattgc ctcggtagta ccgccggtag cgatcaggtc atcaatgata acaaccttct
    12661 gtccaggagt aatggagtcc ttgtgcatct caatctctgc agtgccatat tccagtgcat
    12721 actttgcaga aacagtctcg caaggcagtt ttcctttttt gcggaccgga acaaagccct
    12781 tgtgctctgc ataggcaacc ggcacaccaa agataaatcc tctggactca ggacctacta
    12841 ctacatcata atctacatcc tttaacatat tgcggatact gtccactgcc aggtgcagac
    12901 cgtctgcatc ctgaaggatg gtagttacgt ccctgaaaat aataccaggt tccggaaaat
    12961 ccgggatgct tcttacatat tcttccagct ttttcatatt tatatcctcg tttctttaaa
    13021 ttatttacta tgaaagtgtt gccaagcggc tatggggact gcgtgcttgc acgcaaggaa
    13081 ccatagacat ttggcaacca agccggtatg agtaagcagg gcgacagccc ggattacgaa
    13141 tactggcggc agttgtggga gtgccagcgg cacgtaacaa ctgactttca tctatggtgc
    13201 acaatcacta ttattatact tgctctgacg gacactgtaa accccaggca tcttcccaaa
    13261 ccgccatttt tcctgtattt tctgccagtc cgaccgctgt atagactcct cttttccctt
    13321 aaatctttat tcccatctcc ccgaaaatat ggtaatatgt ccttgttatt ttcaaaacac
    13381 attgagatat tgtgaggttt tacatatgac tggcttaggt acacttgtaa atatggctgc
    13441 catacttata ggaagcggcc tgggactttt attaaaaaat ggtattccaa agcggctgca
    13501 gactactatt tccagcgcag ttggcctttg tttgatcttt gtaggcatta caggggctat
    13561 caagggtctg atgaacgtat caggaactac ctttgagacc aaagatacgt tagtggtagt
    13621 catctgcatg gcaatcggcg ctgccatcgg tgaatgtctg gatctggaac agagaatgga
    13681 aaacctgggg gactgggtca aatccaagct tccaaatggt tcctcttcct ccaattttac
    13741 agaagccttt gtaagcacct cccttgtttt ctgcatcggt gccatggcca tcgtcggttc
    13801 cattgaagac ggtttaaacc atgattatac catgctcttt acaaaagctg ttatggacgg
    13861 cgtgctttcc attgtttttg tagcatcctt aggtatcggc gctgcatttt ccatgtttcc
    13921 cgtaggcatc taccagggaa gtatcactct tcttgcaggt ctggtaaaac cctatcttac
    13981 agacctgatg atcggacgcc tttcctgtat cggttctatc cttatctttg cccttggttt
    14041 aaatctgacc attggaacaa agatcaagat cgcaaatatg cttccggctg tattcctgcc
    14101 ggttatcgta tgcctgttgg gattttaaaa caggttatgg ttgtcattgt gaatatgatt
    14161 atgaaaggac attatcatgt taaaagaaga aagacagacc tggatcttaa ataaacttca
    14221 ttcctccgga aaggtagtgg tcagccacct ggccggggaa ttaaaggttt ccgaagatac
    14281 catacgccgt gacctgttag acctggacca gaaaggacag ttaaaacggg tatttggcgg
    14341 tgccattcct ctggaaaaac cggtcatcaa cttctttgac cgggaaaccg cggatgttgc
    14401 cttaaaacag cagcttgcca tgcgggcatt ggaatttctg gagccggacc agctggttgc
    14461 cattgacggc agcagcacca atcttcagtt tgccaaaaat atccctgata atttaaagct
    14521 tacagttctt accaacagct acagcattgc ccatgtatgc tccttaaaag agcagatccg
    14581 tgttattatc ctgggcggtg atctgttaaa agactccatg accacagtag gggaagtagc
    14641 cgctgcccag gctgctgcct atcacccgga tctgtgcttc atgggtgtct atgccatcca
    14701 cccggaatac ggcatgacca ttccctatcc agaagaagtc agcatcaaac gtcagctgat
    14761 ccgctcctcc agccgtgtga tcgcactggt aaatcccatc aaactgaaca ctgtttcacg
    14821 ataccaggtg tgtgggatcg aggattttac aactctcatc acagatggaa atgtgccgca
    14881 ggagatggca tcacgatata aaaacagagg attagatttc ctgtaaaaca gtttgagccg
    14941 cccggtatgg gcggctctta cttcttccaa tcaaaaaaac tgcgtcaaaa tgaccgttcg
    15001 atttgcaaat atcaattgta cacatcttga gcgcagctgt acgaatgtag cggacaaagt
    15061 ccgatacagg cgaacaggtg catgcgaaag ccagtgtaca atgatacttg caaatcggtt
    15121 tcaggtgatt ttgacgcagt tttttcctct actttattca ccaaatactg ccttataatc
    15181 agcctggaac ttggcaattc cctggtcagt taaaggatgc ttggtcatct gctctaatac
    15241 cttgtatgga acagttgcaa tatcagcgcc agcctttgcg cagtcgatca catggatcgg
    15301 gttgcgaaca cttgctgcaa tgatctcagt ctcgatgttg tgcatctgga agatctcggt
    15361 gatatcatag atcaggtcaa tacctggcat ggagatatca tccagacgtc ctaagaatgg
    15421 agatacatag gtagcgcctg ctcttgcagc taacagtgcc tgtgctgcgg agaaaatcag
    15481 agttacgttg gtcttaatgc cttctgcgtg aagaacctta actgccttta agccttctac
    15541 agtcataggg atcttaacaa ccatgtttgg atggatctta gctatctcac ggccttcttc
    15601 gatcattcct tctgctgtta cagtagttgc ctttacttcg ccgctgatcg gtccgtcaac
    15661 gatagaagtg atctctttga taacctcttt aaaatcacgg ccttcttttg cgatcaggga
    15721 tgggtttgtg gtaacaccac agataattcc catatcattt gccttgcgga tatcatctac
    15781 atttgctgtg tcaataaaaa acttcatgaa atagtcctcc tcttacattt tgcttcccac
    15841 cgataccgga tttctcttcg acgaagacgg gaaagcatac attgtgaaca atgacggtcc
    15901 ggcaggaaag ccc
//
LOCUS       DBMDUA010000049        15367 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2135_length_15367_cov_2.635661, whole genome shotgun sequence.
ACCESSION   DBMDUA010000049 DBMDUA010000000
VERSION     DBMDUA010000049.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 15367)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15367)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15367
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2135_length_15367_cov_2.635661"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="ACZPCL_05610"
     CDS             <1..194
                     /locus_tag="ACZPCL_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HIR1161986.1"
                     /translation="SKYYELINSQQDYIEVRSQNWLPSYSVIDLTKDDFKITTYQITD
                     EGKVENIDNTFTIHKTVNR"
     gene            317..1444
                     /locus_tag="ACZPCL_05615"
     CDS             317..1444
                     /locus_tag="ACZPCL_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HIR1161987.1"
                     /translation="MDQALKKQVKKYAVMTVIMVIMALAVVKLNNVTKTQLVNRTGQT
                     FETGKVVRILEDNIQEDGMRVGQQTVVVKMTSGVKKGQELTTTSSAGYLFGAACKVGM
                     RVVVLQSVAGNSVITSVYSMDRKEVMIGFVVLYLLALCLVGGLQGVKGALGLIFTFGA
                     ILFIYLPVVYMGYSPFWTAVFICAITTAVTMYLIGGASEKTVCAVLGTISGVLIAGIT
                     ATVFSRLSGITGWNVSDIESLLTLYETNDIQVGGLLFSGLLISTLGAVMDVAMSISSA
                     MKEIYDQNHQITRMQLVKAGMRVGRDMMGTDSNTLILAFAGSSISMLIMDYAYHLPYL
                     QIINSNNIGIALMQGLSGSFGIVLSVPATVAFAAWIYTGRK"
     gene            complement(1472..2416)
                     /locus_tag="ACZPCL_05620"
     CDS             complement(1472..2416)
                     /locus_tag="ACZPCL_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1161988.1"
                     /translation="MELKEARYILAIAKHQSIGKAAESLYISQPSLSKYLKNLEERLG
                     APLFDRRENHYYPTYMGERYLYYAERIAAFGDQWSQEFDDIAHQEKGRLNIAAPIMMG
                     TTLIEPSLMGFHKRYPYVTLNIMEAINFVAENSLEDNSIDLTIYNVHKFPENMDYQIL
                     REEEIVLVLNKNHPLCEKAQPKEGFRHPWLDLKMLEKENFILLYPDQNTGGIAQSLFK
                     EYSMEPTVLLHTRNSAMSIRLAIQGLGAAFAPESYFREESRFFPEGCCFSIGQKPVLT
                     TTIVAYQKKKYLSQHARDYVEILKEYATSLSVSKGNDI"
     gene            2597..3430
                     /locus_tag="ACZPCL_05625"
     CDS             2597..3430
                     /locus_tag="ACZPCL_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161989.1"
                     /translation="MKKSSAWFMLTPGLVILFLFLAIPLLRVLFPSIFSGAYPFDAYV
                     GFFQDEYYRKIFIRTVKIAVITTFVCMVGGIPTAYFISRCSKKWRGILLAASIFPMMT
                     NSVIRSFAWINILGSNGIINKVLVGLGVVPKPLKLLYTDFSIIIGSVYLFLPLMIVTV
                     AGVMENIDDDMMEAALSLGAARMEAFMKVIFPMSLPGIIVGGILVFTGTLTAYTTPQL
                     LGGNSNMVMATLIYQRAMSVSDWTGASVIALIMIVVTLAVIRGFNALAAKLDRRGENY
                     A"
     gene            3423..4205
                     /locus_tag="ACZPCL_05630"
     CDS             3423..4205
                     /locus_tag="ACZPCL_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1161990.1"
                     /translation="MRKNKLLTIWAVLVFAFLAIPLLIITVTAFGGGSAITFPIESFS
                     LKWFANVFALKSFRRSFITSLEVAFLATFISLLVGIPAAYALARSGMKGRGLLKSIFL
                     SPTIVPGIVIGFIMYQCLILTLRIPVFTGLLAGHFMVTLPYVIRVVGSSMEQFDFSIE
                     EAAWSLGCPKTAAFFKVVLPNVTSGISSAFMLAFINSFNNIPVSMFLSGPGVSTFPST
                     LMNYIEYNYDPTVSAVSVLLMAATVIIMVIVDRTLGIAALAK"
     gene            4255..5295
                     /locus_tag="ACZPCL_05635"
     CDS             4255..5295
                     /locus_tag="ACZPCL_05635"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1161991.1"
                     /translation="MAFMSLKDIDVSYDKKKQILKGLSLDVEKGELVSLLGPSGCGKS
                     TTLRVVAGFIDPQGGSFLLDGEDMTKVPVHKRNFGLVFQSYALFPHLSVYDNVAFGLR
                     TRKMDKELIDKKVKDILEVCGLSDLANRFPRQMSGGQRQRVALARSLVIEPKLLLLDE
                     PLSNLDAKLRLSMRVEIKRLQKKLGITTLFVTHDQEECFSISDKVAVMNGGVIEQYDT
                     PENIYRRPATEFVARFIGFENFLDVEKKADHLYVLSDGTAFKTAMDLDGGEYAATIRP
                     DDIRLADDKFSENVLSGKVGVRTFLGKSYQYEVETAAGVLKVNMGTDHVFKEGDMLRL
                     YLPEDKLILVRR"
     gene            5377..6480
                     /locus_tag="ACZPCL_05640"
     CDS             5377..6480
                     /locus_tag="ACZPCL_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1161992.1"
                     /translation="MKKRLAVVLCAAMMLSACGGASTSDKTADTNGQTAADSKADATE
                     GSKGDGKLVLSTYGLSEDISEDEVYTPFEQEFGCKIVTETGGTNDRYTKLAADSESSI
                     DVIELSQAMTAKGADEGLFDTIDLSKIPNAENLIPAAKELAENGQGIPYTINSIGIIY
                     NPELTGFEIKSFDDLWDSRLEGMVSIPEITTTFGPAMVYVAGDHAGTPVAEDEGKAAF
                     EALAELKPNLVKTYTKSSDLINMFTSGEVAAAVVGDFGVPTIVAANPDLVYVTPEGTY
                     ANFNTINITKNCKNKDLAYEYLNYRISPELQTKTGKALNEAPTNKEVTFTEEEAKDMT
                     YGETADNAKIVDYSFVNPILNNWIDQWNRTINQ"
     gene            6491..8257
                     /locus_tag="ACZPCL_05645"
     CDS             6491..8257
                     /locus_tag="ACZPCL_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1161993.1"
                     /translation="MTADLIIKNANVFMTYRQCFEKRDVAVAGEKFYCVSPAIVYPGV
                     KTIDARGKYMIPGFIDIHMHIESSMTTPVEFSRTTLPYGVTTVVADAHEIANVFGMEG
                     IKSFMSQKTEQDIFWAIPSSVPATNEELETPGAALYPEDAKKMASDPGVLCLGEIMNF
                     KDLSADGYSKTREMIRLCKEEAPLMRIEGHCPGLSGEDLNRFIYEGVDADHTQQTPQS
                     VLEKTDLGMFLELQLKSLRPEVVKVVCENQLYENVALVTDDTMADKLVKSQLNGIVKA
                     AIEAGMPVEKAIYCATWTPARRMHLDDRGMIAPGKIADFALLESLKDMQPVMVFKKGC
                     CVYEKGALEKGQADMQAEIQEQRFLSVGDFPEHFYHSVQCREAEKEDFQIKAEDPSAA
                     FAEVNVIKISDFGTATTPVKKRLSIKNGNICWKEAGLSLAVVYERYGKNGNVAKALVE
                     GTFKEPGAAATTWSHDSHNLLVMGNNEEDMELAQKRVRELQGGYVVYAGGKLAAQAPL
                     PIGGILSDQPVSVLGKQLGQVRKAMEDLGYDNNNVIMSMSTLCLPVSPRLKLTDFGLL
                     EVKSQEKVPLIQTYFDKNGMRI"
     gene            8260..9600
                     /locus_tag="ACZPCL_05650"
     CDS             8260..9600
                     /locus_tag="ACZPCL_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1161994.1"
                     /translation="MKTIIQNGIVVTMDQQMQVWDHGYVIIDGTKIVEAGPESELELK
                     KAALARVGENWQEKDACRGIIMPGMVNTHSHLGMIPFRGLGDDCKDRLRVFLLPMESQ
                     AMDAELAAASARYGICELLLSGVTTVLDMYYFEEKVAHIMDQMGIRGIAGETVMEDAT
                     CDAYCAEEALERGRDLIRAYRNHPRVKGALTPHGTTTCSGEHMKMALEEDLKGNTVFT
                     LHTAEMDYEMTYLAEKYQMTPVEYLNSFELLGAHTLTAHSIRLTEADCRLLAETKTNV
                     AHCIGSNTKAAKGVAPVALLRSLGVNVGLGTDGPASGNTLDLFTQMRMCENFHKNTLK
                     DRSAFPAKEIVKMATIEGAKALGLGDVTGSLEPGKEADLVIIETDSPNMFPIYDPYST
                     LVYSAFASNVRDVYVAGECLVEEKRLVKEDLMDVRMALKEKMERTKFKEMKALT"
     gene            complement(9679..10332)
                     /locus_tag="ACZPCL_05655"
     CDS             complement(9679..10332)
                     /locus_tag="ACZPCL_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HIR1161995.1"
                     /translation="MNQKKVTLRGELALLIVILINSMGVVLMLRSGSGISAISSVPYA
                     FSLVFPALTLGTWTYIFQGLLVLSLMILKKRFVPSYLFSFVAGFAFGKMVDIHEIWIN
                     TLPLTIPLRVFYFVASYLILCFGIALSNRCKLPVIPTDLFPRDLSEIIKKPYSKVKIT
                     FDVTCLFITACMTFLCLGHISGLGIGTVAAAFTMGKTIGVIGDAIDKKVEFVSVLSK"
     gene            complement(10529..11761)
                     /locus_tag="ACZPCL_05660"
     CDS             complement(10529..11761)
                     /locus_tag="ACZPCL_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1161996.1"
                     /translation="MALHVMDEANRCLGCKVPQCQKGCPISTPIPEVIKLLKANKLDE
                     AGKMLFENNPLTTVCSLVCNHENQCEGHCVLGRKGAPVHFSTIESYISTTYANKMTQG
                     PAPSNGMRAAIIGSGPAGLTIAVILARYGYDVTIFEGKDKVGGVLRYGIPEFRLPKSV
                     LDDFKYRHLDLKGIKFRPNTHIGGAIGIDDLFRDGYKAIFVGTGVWKPNALHIKGETL
                     GNVHFGINYLNNPDSYQLGKRVIVIGAGNAAMDVARTAIRKGVQELTCFSITKQVAAS
                     KHEFSYAQLEGVQFEYNMAPVEIKDDGVIFKDVIENEDGTFTDVPDSTHFFPADSVII
                     SISQGPQNRIVNTTQGLAADKRGLLVADETGHTTRPGIFASGDVVNGARTVVEAVAHS
                     KIVAESMHQYMQSLKDGE"
     gene            12032..12931
                     /locus_tag="ACZPCL_05665"
     CDS             12032..12931
                     /locus_tag="ACZPCL_05665"
                     /inference="COORDINATES: protein motif:HMM:NF018584.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HIR1161997.1"
                     /translation="MDKKFLRHISSMMLLVCTLAAVSPMTAFAGWQKEGDTYAYYEDD
                     GTRVENAFRKSGNQWFYLDENGHLLKNCEREIDGKIYVFNERGAVVPSATRKAGSSES
                     SESDFSAISSRQQYLNKTANPYMNNKTVQWINATYAILTKHNGGNIRAFGGSLKVNGK
                     EENGAEIDKTTRDQTRKMLKQSWGVTDRDSADEALNELLESAKESGSAWDYSRAESNL
                     GFYYLADYYTEKEALDKALEVAKEIQSTFESWDSFVQSYLEGYEAGSGDTSGDREEIY
                     ENLKACQWNPYSVSWNTKLKKNW"
     gene            12941..13924
                     /locus_tag="ACZPCL_05670"
     CDS             12941..13924
                     /locus_tag="ACZPCL_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR1161998.1"
                     /translation="MKWGILATGTIAEKFADTVNHMEQEDETLIACASRNLEHGKAFA
                     SKYGIPKVYGSYAQMMTDSEVDAVYIATPNSLHYENCRMCLEAGKHVLCEKPFTLCVD
                     QAEELYELAEEKGLFIMEAFWIRFLPAYDKLRAMLRDGVIGEVKRITSQFGFTVEGAR
                     RERKFKSELGGGALLDIGIYNLGFFHMITEAAPEGFQSEVHMTEYGTDDYSEVELEYP
                     GGCSAHCIQAIGKKLERHAKIEGTKGDILMDDFQHLQEFTVQLKDGSSYLVKEPFKVN
                     GFEYEIVETSRCVSCGLNTSDRYTKEDCLTILRLMDDIRESWDMTFEGEEK"
     gene            14072..>15367
                     /locus_tag="ACZPCL_05675"
     CDS             14072..>15367
                     /locus_tag="ACZPCL_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1161999.1"
                     /translation="MTIFEYLYGDVYYTDEYGNAYFDSCVDIDYELDEIPKIVNLGDH
                     TYFMAKEDLDLYNQYDIKVDGVSEDDLRFLHYTRRPYYQMRGRSVSREQAFDIIRRTD
                     NFFNWDMETIGNRKEFVRCINFDNWLIMKNHYPKGYGWIHADGTVGANAITQKWPTMI
                     ELVTEWFYKLKSFPYLDLVIGITNWDEISWDEDDTFEKAIQMGIYVHDKCIELLNKQN
                     AWAKYQEYDEKYGADPERFETDYYQKNGIVQVDEAYLRKCIESYGLDPDEELSKVRPY
                     IWKGEIMEENKMIYKNEDGNYHIRILMSQEQCKQLKLAYPLEVDVCVCEAERRKEWEE
                     SIREKIEDALYEMVAPVDEDDFYSDVYRAWFEEIAWNWELEKEGFLPEEKDPFSEINM
                     DRTLLAYRRLCACLTIVGKQKEIERVFLLSRRLHLCVPHG"
BASE COUNT         4491 a         2969 c         3827 g         4080 t
ORIGIN      
        1 gttccaagta ttatgagctt attaattcac agcaggatta cattgaagta agaagccaga
       61 actggttgcc aagctactct gtgatcgatc tgaccaagga tgatttcaag atcaccactt
      121 atcagatcac agatgaagga aaggtagaaa atattgataa tacctttacc attcacaaga
      181 ccgtaaacag ataagcccaa agccggcaat aaaaggtata aaaaagatat aaaaagacat
      241 aaaagatcaa attccgtccc gcgaaagctg caaaaagcct tgcgggacgt tgatcggata
      301 gatcgaggca gtaaaaatgg atcaggcact gaaaaaacag gtaaaaaagt acgcagttat
      361 gacagtgatc atggtgatca tggcactggc agtggtaaaa ctgaataacg taactaagac
      421 acagctggtc aaccgcacag ggcagacttt tgagacagga aaagtggtcc gtatcctgga
      481 agacaacatt caggaagacg gaatgcgtgt ggggcagcag actgtagtcg taaagatgac
      541 gagtggagtg aaaaagggac aggaacttac aactaccagc agcgcaggct atctttttgg
      601 tgcagcctgc aaagtgggaa tgcgggtagt ggttctccag agcgttgcag gaaattcagt
      661 gatcacaagc gtttattcca tggaccggaa agaggtaatg atcggttttg tggtacttta
      721 tctgctggca ttgtgcctgg taggcggttt gcagggcgta aaaggtgctc tgggactgat
      781 ctttactttt ggggccattc tttttattta tcttcccgta gtctatatgg gatattcccc
      841 tttctggaca gccgtattta tatgtgccat taccaccgca gtgaccatgt accttatagg
      901 cggtgcttct gaaaaaacag tctgtgcagt ccttgggacc atatcaggag tccttattgc
      961 cgggatcaca gctactgtat tttccagatt atcagggatc acagggtgga atgtatcaga
     1021 tatagaaagt cttcttactc tttatgagac caatgatata caggtaggcg gactcttatt
     1081 ttccggtctt ctgattagta ccttaggggc agttatggac gtagctatgt ctatttccag
     1141 tgccatgaaa gagatttatg accagaacca ccagattacc cgcatgcagc tggtgaaagc
     1201 aggaatgagg gtgggccgtg atatgatggg aacagattcc aatacgctga tccttgcttt
     1261 tgcaggcagc agtatttcca tgctgatcat ggattatgcc tatcatcttc cttatcttca
     1321 gatcattaat tccaataaca tcggtatcgc tttgatgcag ggactttcag gaagtttcgg
     1381 tatcgtatta agtgttccgg ctacagtggc attcgccgca tggatctata caggaagaaa
     1441 ataagataga tcattttgtt taaaatattt tttatatatc gttgcccttt gaaaccgata
     1501 gggaagtagc atattctttt aagatctcca catagtctct tgcatgctgg gaaaggtatt
     1561 tctttttctg gtatgctaca atggttgtgg taagaaccgg tttctggcca atggagaagc
     1621 agcatccttc cgggaaaaac cgggattctt cccgaaaata actttccggt gcaaaagcag
     1681 ctcccagtcc ctgaatcgca aggcggatgg acatggcact gtttctggtg tgaaggagta
     1741 ccgttggctc catagagtat tctttaaata aagactgtgc gataccaccg gtattctggt
     1801 ccgggtataa caggataaaa ttttcttttt caagcatttt caagtccagc catgggtggc
     1861 gaaagccttc ctttggctgg gctttttcac ataaggggtg gtttttattt aagaccagta
     1921 cgatctcttc ttctcttaat atctgataat ccatattttc cggaaattta tgtacattat
     1981 agatcgtcag gtcaatggag ttgtcctcca ggctgttttc cgcaacgaaa ttaatagctt
     2041 ccataatgtt taaggtaaca taaggatagc gcttgtgaaa gcccataagg gatggctcga
     2101 tgagagtagt acccatcata atgggtgcgg caatatttaa gcgtcccttt tcctggtggg
     2161 caatgtcatc aaattcctgg ctccactggt ccccaaaggc ggcaatgcgc tctgcgtagt
     2221 agagatagcg ctcccccatg taagtgggat agtagtggtt ttccctgcgg tcaaataagg
     2281 gagcgcccag gcgttcttcc aggtttttta aatatttgct taaagagggc tgggaaatat
     2341 acagggattc tgcggctttt ccaatgctct ggtgttttgc aatggcaaga atatagcggg
     2401 cttcttttaa ttccataggt gtcctttcca aatttatatt atagctttac tgcataaaaa
     2461 ttatagaaaa tatatatttg acttatagta taccactttc tataatgtat gtgaaatata
     2521 taagtacatt tcattaagaa ataaatattt attatgtatg ctaattaata tttatcagag
     2581 aggagaaaca aaaacaatga aaaagagcag tgcatggttt atgctcacac cgggactggt
     2641 gatcctgttt ttatttctgg cgatcccgct gcttcgggta ttgtttccaa gtatcttttc
     2701 cggagcatat ccctttgatg cctatgtggg atttttccag gatgagtatt acaggaaaat
     2761 ctttatccgt actgtaaaga tcgcagtgat cactaccttt gtctgcatgg tgggcggtat
     2821 cccaaccgct tattttatca gccggtgcag caagaaatgg agagggatcc tccttgcagc
     2881 ttccattttc ccaatgatga ccaactctgt aatacgaagc tttgcatgga tcaatatcct
     2941 gggaagcaac ggcattatca acaaagtgct ggttggactg ggagtggtgc caaagccatt
     3001 aaaactgctg tatacagact tttccattat cattggttct gtgtatctgt ttcttcctct
     3061 gatgatcgta acagtagcag gtgttatgga aaatattgac gatgacatga tggaagcagc
     3121 cttaagtctt ggggcagccc gtatggaagc ttttatgaag gtgatctttc ctatgagtct
     3181 tccgggtatc atcgtaggag gcatcctggt atttaccgga acactgactg cttatacaac
     3241 gcctcagtta ttgggaggca actccaacat ggtaatggcg accctgatct atcagcgtgc
     3301 catgtccgtc agtgactgga ccggtgcttc tgtgatcgcg ctgatcatga tcgttgtaac
     3361 actggctgtg atccggggct ttaatgctct ggcagctaaa ctggaccgga gaggtgagaa
     3421 ttatgcgtaa aaataaactt ttgaccatat gggcagtgct ggtatttgcg tttcttgcta
     3481 ttcctctgct gatcattaca gtaacagcct ttggaggcgg cagcgccatt acatttccca
     3541 ttgagtcttt ttcattaaaa tggtttgcca atgtattcgc cttaaaatcc ttccgccggt
     3601 cctttatcac cagcctggaa gtggcatttc tggcaacttt tatctccctt ttggttggta
     3661 ttcccgcagc ctatgccctg gcaagatccg gcatgaaagg gagaggactt ttaaaatcca
     3721 ttttcctgtc acctaccatt gtgccaggta tcgttatcgg ctttatcatg taccagtgcc
     3781 tgatattaac tttaaggatc cctgtattta caggactttt ggcaggtcat ttcatggtaa
     3841 cactgccata tgtgatccgt gtggtaggtt cttccatgga gcagtttgat ttttctattg
     3901 aagaagcagc ctggagtctt ggatgtccga agaccgccgc tttctttaag gtagttcttc
     3961 caaatgtaac aagcggtatt tcttccgcgt ttatgctggc atttatcaat tcctttaata
     4021 atatcccggt atccatgttt ttatcaggtc ctggcgtgtc caccttcccg tcaaccctga
     4081 tgaattacat tgagtacaac tatgatccta cagtttctgc tgtatccgta cttttaatgg
     4141 cagcgacggt tatcatcatg gtcattgtag accgtacact gggtattgca gctctggcaa
     4201 aataagttac aggttataac agcgccggtc aggcggataa aggagaaaat gaaaatggca
     4261 tttatgagtt taaaggatat tgatgttagt tacgataaga aaaagcagat cttaaagggc
     4321 ttaagcctgg atgtggaaaa aggagagctg gtgtctttac ttggtcccag cggctgcgga
     4381 aaatctacca ctcttcgtgt agtagcaggt tttattgatc cacagggcgg aagcttttta
     4441 ctggatgggg aagatatgac aaaggttccg gtccacaaga gaaatttcgg actggtattt
     4501 cagagttatg cattattccc gcatctttcc gtgtatgata acgtggcttt tggccttcgc
     4561 accagaaaaa tggataagga actgatcgat aaaaaggtaa aggatatttt ggaagtatgc
     4621 ggccttagcg acctggcaaa ccgcttccca agacagatgt caggtggcca gagacagaga
     4681 gtggctttag cccgttcctt agttattgag ccgaagcttc ttttattaga tgagccgtta
     4741 tccaatctgg atgcaaagct gcgtctttcc atgcgtgtgg aaatcaagcg cctgcagaaa
     4801 aagttaggta tcacaacttt atttgtaacc catgatcagg aagaatgttt ctctatttcc
     4861 gataaagtgg cagttatgaa tggtggtgtc atcgaacagt atgacacacc ggaaaatatc
     4921 tatcgcagac ctgcaacgga gtttgtagca cgttttattg gatttgagaa tttcctggat
     4981 gtagagaaaa aggcagatca tttatatgtt ctctcagatg gaactgcatt taagacagcc
     5041 atggatttag acggcgggga atatgcggca accatccgcc cggatgatat ccgtctggca
     5101 gatgataagt tttccgaaaa tgtactgagc ggcaaagtag gtgttcgcac cttccttgga
     5161 aagagttatc agtatgaagt agagacagca gccggtgttt taaaggtaaa tatgggtaca
     5221 gaccatgtat ttaaagaggg cgatatgctc cgtctgtatc ttccggagga taagctgatc
     5281 ctggtgagaa gataaataca agattaagta agatccggca ttaaaaagca tttacgtaaa
     5341 aatctttata aatctttata taaaaggaga attattatga aaaagagatt agcagtagta
     5401 ctttgtgcag caatgatgtt atctgcatgc ggcggcgcat ccacctccga taagacagca
     5461 gataccaacg gacagacagc agcagactcc aaggcagatg ctacggaagg ctctaaggga
     5521 gatggaaagc tggtcctttc tacttatgga ctgagcgaag atatttctga agatgaagta
     5581 tatactcctt ttgagcagga atttggctgc aagatcgtaa cagaaacagg cggaaccaat
     5641 gaccgttata ccaagctggc agcagacagc gagtcttcta ttgatgtgat cgagctttcc
     5701 caggctatga ctgcaaaggg cgcagatgag ggacttttcg ataccattga cttaagcaag
     5761 atcccgaatg cagaaaacct gatcccggct gcaaaagagc tggctgaaaa tggacagggc
     5821 attccttaca ccatcaacag catcggtatc atttacaatc cggaactgac cggctttgag
     5881 atcaagagtt ttgatgatct gtgggactcc cgcctggaag gtatggtttc tattcctgag
     5941 atcacaacta ctttcggacc ggctatggtt tatgtagcag gtgaccatgc aggtactcct
     6001 gtagcagagg atgaaggaaa ggcagctttt gaagcactgg ctgagttaaa accaaacctt
     6061 gttaagactt atacaaagtc ttctgacctg atcaatatgt ttacttccgg tgaagtagca
     6121 gcagctgttg taggtgattt cggtgttcct actattgtag cagcaaaccc ggatctggta
     6181 tatgtaactc cagagggaac ttacgcaaac ttcaatacca ttaacattac caagaactgc
     6241 aagaacaaag atctggctta tgagtactta aactatcgca tcagccctga actgcagaca
     6301 aagactggta aggcattaaa tgaggctcct accaacaagg aagttacctt tactgaggaa
     6361 gaagcaaagg atatgaccta cggcgagact gctgacaacg caaagatcgt agattattct
     6421 ttcgtaaacc cgatcttaaa caactggatc gaccagtgga acagaaccat caatcagtag
     6481 gagaaaatat atgaccgctg atcttattat taaaaacgca aatgtcttta tgacctacag
     6541 acagtgtttt gaaaagcggg atgtggcagt tgcaggggaa aagttttatt gcgtttcccc
     6601 tgcaattgtt tatcctgggg taaagactat tgacgcccgg ggaaaatata tgatcccggg
     6661 gtttatagac atccacatgc acattgaaag ttctatgaca actcctgtgg aattttccag
     6721 gaccacactt ccctatggag tgactaccgt tgtggcagac gcccatgaga tcgccaatgt
     6781 atttggcatg gaaggcataa aaagctttat gagccagaaa acggagcagg acattttctg
     6841 ggcgatccct tccagtgtac cggctaccaa tgaagaactg gagacaccgg gagctgctct
     6901 ttatcctgag gatgcgaaga aaatggcttc ggatccaggg gttctctgtt tgggagagat
     6961 tatgaatttt aaggatctca gtgctgacgg gtacagtaag accagggaaa tgatccggct
     7021 ttgcaaagaa gaggcgccgt tgatgcgcat tgagggtcat tgcccgggac tttcgggaga
     7081 ggatctgaac cgctttatct atgagggcgt agatgcggac catacccagc agaccccgca
     7141 gtcggtattg gaaaaaacgg atcttggcat gtttctggag ctgcagctta agtctttgcg
     7201 gcctgaggtg gtaaaggtgg tctgtgaaaa tcagctgtat gaaaatgtag cgctggtgac
     7261 agacgatacc atggcagata aactggtaaa gagccagtta aacgggatcg taaaggcagc
     7321 cattgaggct gggatgcctg tagaaaaggc aatttactgt gctacctgga cacctgcaag
     7381 gcggatgcat ttagatgacc gtggtatgat cgcaccggga aagatcgctg attttgctct
     7441 tttggagtct cttaaggaca tgcagccggt tatggtattt aaaaagggat gctgtgtgta
     7501 tgaaaagggt gcgttggaga aagggcaggc agatatgcag gcggaaattc aggaacaaag
     7561 gttcctttct gtgggagatt ttccagagca tttttatcat tcggtccagt gccgggaggc
     7621 tgaaaaagag gattttcaga tcaaagcgga agatccgtca gctgcatttg cagaagtaaa
     7681 tgtgataaaa atatcagatt ttggtacggc taccacaccg gttaaaaaga gattatccat
     7741 taaaaatgga aatatctgct ggaaggaagc aggactttcc ctggcagtgg tttatgagag
     7801 atatgggaaa aatggaaatg ttgccaaagc actggtggaa ggtactttta aggagccggg
     7861 agcagcagct actacctggt cccatgacag ccacaatctt cttgtaatgg gaaataatga
     7921 ggaagatatg gaacttgccc agaaacgggt gagagagctt caggggggct atgtggtgta
     7981 tgccggcgga aagctggcgg cccaggcccc tcttcctata ggcggtattt tatcggatca
     8041 gcctgtttct gtgttaggca aacagctggg acaggtgcga aaggccatgg aagatctggg
     8101 ctatgacaac aacaatgtga tcatgtccat gtctacttta tgtcttcctg tatccccaag
     8161 actgaagctg acggatttcg ggctgttaga ggtgaagagc caggagaaag tgcctctgat
     8221 ccaaacttat tttgataaaa acggaatgag gatctgaaag tgaaaacgat cattcaaaat
     8281 ggaattgtag tgaccatgga ccagcagatg caagtgtggg atcatggtta tgtgataata
     8341 gatggaacaa aaatcgtgga agcagggccg gagagtgaac tggaattaaa aaaagcggct
     8401 ctggcccggg tgggagaaaa ctggcaggaa aaggatgcat gcagaggcat cattatgccg
     8461 ggaatggtca atacccacag ccacttaggg atgatcccgt tcaggggact gggagatgac
     8521 tgcaaggacc gcctgcgggt atttctgctt cctatggagt cgcaggcaat ggatgccgaa
     8581 ctggcagcag catctgccag atacggtatc tgtgaactgc ttctttccgg tgtgaccaca
     8641 gttcttgata tgtattattt tgaagaaaaa gtagcacata tcatggatca gatgggaata
     8701 cgtggaattg cgggggagac cgtaatggaa gatgctacct gtgacgccta ctgtgcagag
     8761 gaagcgctgg aacggggccg ggacctgatc cgggcttaca gaaaccaccc aagagtgaaa
     8821 ggggcgctga ctcctcatgg aaccactacc tgcagcgggg aacatatgaa aatggctctg
     8881 gaagaagatc ttaagggaaa tacggtattt acactccata ctgcggaaat ggattacgaa
     8941 atgacgtatc tggcagaaaa ataccagatg acaccggtgg agtatctgaa ttcttttgaa
     9001 cttttgggag cgcatacgct gactgcccac agtatccgcc tgacagaagc cgactgcagg
     9061 ctgttagcag aaacgaaaac caatgttgcc cactgtatcg gttccaacac caaggcagcc
     9121 aaaggagtag cgccggtggc tctgcttcgc agcctggggg taaatgtagg tctgggcaca
     9181 gacggaccag ccagcggaaa cactctggat ctgtttaccc agatgcgtat gtgtgaaaat
     9241 ttccacaaaa acaccttaaa agaccgaagt gcttttcctg caaaagagat cgtgaaaatg
     9301 gcgacaattg agggagccaa agctttggga ttgggagatg tgaccggttc cctggaacct
     9361 ggaaaagagg ctgatctggt aatcatagaa accgactccc ccaacatgtt ccccatatac
     9421 gacccctact ccaccctggt ctacagcgcc ttcgcctcca atgtacggga tgtgtatgta
     9481 gccggagaat gcctggtaga agaaaaacgg ctggtaaaag aagatctgat ggatgtccgg
     9541 atggcgttaa aagagaagat ggaacggacg aaatttaaag aaatgaaggc tctgacctga
     9601 tgggttggag tctttttcta tgatagaata gcaaaaagcc gcctgttttg gcggctttta
     9661 gtatgtttat ggttgtattt acttgcttaa tacggataca aattccactt tcttatcaat
     9721 cgcatccccg attacaccaa tggtctttcc catggtgaaa gctgctgcta ctgtaccaat
     9781 tccaagaccg gagatatgtc caaggcagag gaaggtcatg caggcggtaa taaaaagaca
     9841 ggttacgtca aaggtgatct tgactttgga ataaggcttt ttaatgatct cggagagatc
     9901 tcttgggaac aggtcggttg ggatgaccgg gagtttgcag cggttggaaa gggcgatacc
     9961 gaagcagagg atcagatagc ttgccacaaa gtagaacacc cgaaggggaa tggtaagtgg
    10021 cagtgtgttg atccagatct catgaatatc taccattttt ccaaaggcaa aaccggctac
    10081 gaaactaaat aggtagctgg gaacaaaacg tttttttaag atcatcaggc ttaaaacaag
    10141 aagtccctgg aaaatatagg tccaggtgcc aagggtcaaa gccggaaata caagggaaaa
    10201 tgcgtagggg acgctggaaa tagcggaaat gccggagccg gagcgcagca ttaaaacaac
    10261 gcccatgctg ttgatgagga taacgatcag cagggcaagt tctccgcgga gggtgacttt
    10321 tttctgattc ataatgatta ctctcttttc tttttgtaaa ttgatgaaag ttaaaaaata
    10381 tgtacattca caaagtttgt ttgccatttt tataacagaa caaactttga aaacacataa
    10441 aagggggatg cagcgaaaca ttttattttg caacatcccc gattatactc ttggtttata
    10501 agattttcaa gaaaattgtg ttacagtctt attccccatc tttcagactc tgcatatact
    10561 ggtgcataga ctcggcaact attttactgt gagcaactgc ctcaaccaca gtacgtgcgc
    10621 cgttcacaac atcaccggaa gcaaaaatac ccgggcgggt ggtatgtccg gtctcatcgg
    10681 ctaccaacag gcctctttta tctgcagcca gaccctgtgt tgtgttgacg atccggttct
    10741 gcggtccctg gctgatagaa ataatcacgc tgtcagccgg gaagaaatga gtggagtccg
    10801 gaacgtcagt aaaggtgccg tcttcatttt caatcacatc tttaaagata acaccgtcat
    10861 ctttgatctc cacaggagcc atattgtact caaactgaac gccctctaac tgagcataac
    10921 tgaactcatg cttgcttgca gccacctgtt ttgtgataga gaagcaggtc agctcctgaa
    10981 cacccttgcg tatggcagtt cttgcaacgt ccatagctgc gtttccggca ccgatgacaa
    11041 tgacacgttt tcctaactgg tagctgtccg gattgtttag ataattaatg ccgaagtgaa
    11101 catttcccag agtttcccct ttaatatgaa gggcgtttgg cttccacacg ccggttccta
    11161 cgaaaatggc cttatagccg tcacggaaca ggtcatcaat accgatggcg ccgccgatat
    11221 gtgtattcgg acggaattta atgcctttta agtctaagtg gcggtattta aaatcatcca
    11281 ggacagattt tggcagacgg aattccggga taccgtaacg aaggacaccg ccaactttat
    11341 cttttccttc aaaaatagta acatcatagc catatcttgc aaggataaca gcaatggtaa
    11401 ggccggcagg accggaaccg atgatagcag cccgcatacc attggaagga gcaggtccct
    11461 gggtcatttt attggcataa gttgtggaaa tatagctttc aatggtagaa aaatggaccg
    11521 gagcaccctt acgtccgaga acgcagtggc cttcgcactg gttttcatgg ttgcatacaa
    11581 gactgcatac agtagtcagc ggattatttt caaaaagcat tttacctgct tcatctaatt
    11641 tatttgcctt taaaagtttg atcacttccg ggatgggagt gctgatagga caccctttct
    11701 gacactgggg aaccttacat cccagacaac ggtttgcttc atccataaca tgtaatgcca
    11761 tttacaaaac ctccatactc atcataaatc ttagttcata catttttctg gacccattct
    11821 atcacagatt actttttgtg accagtgtct aaaacaatgt atttaattct ataacgaatg
    11881 ataaaaaatg cacaaaaatg tgataaaaaa tgcacaaaga taagttgcaa aaggaaagaa
    11941 tgaaagaatt tcgtcagaaa tgtgtaagtt ggaaaatgga aaggcctgtg ttatactatg
    12001 tttgaggaga tacaacgaga ggaagatcgt tatggataaa aaatttttaa gacacataag
    12061 cagcatgatg cttcttgtct gtacgctggc agcagtcagt cctatgaccg cttttgccgg
    12121 atggcagaaa gaaggcgata cttacgctta ttacgaagac gatgggacca gagtggaaaa
    12181 tgctttccgc aaatcaggaa accagtggtt ctatctggat gaaaatggcc atcttttaaa
    12241 gaactgtgaa cgtgagatcg atggaaagat ctacgttttc aatgaaagag gtgcagtggt
    12301 tccttccgct accagaaaag caggaagcag tgaaagttct gagtctgatt tttctgccat
    12361 cagcagcaga cagcagtatc tgaacaaaac agcaaatcct tatatgaaca ataagaccgt
    12421 tcagtggatc aatgctacct atgccattct tacaaagcac aatggcggaa atatccgtgc
    12481 ttttggcgga agccttaaag taaatggcaa agaagaaaat ggtgctgaga tcgacaagac
    12541 taccagagat cagacccgca agatgttaaa acagagctgg ggtgttactg acagggattc
    12601 tgcagatgag gccttaaatg aactcttgga gtcagcaaag gaaagcggtt ctgcctggga
    12661 ttacagcagg gcagagtcta atctgggatt ttattatctg gcagattatt acacagaaaa
    12721 agaggcttta gataaggcac tggaagttgc aaaggagatc cagagtacct ttgaatcctg
    12781 ggactctttt gtacagagct atctggaagg ttatgaagca ggctccggtg atacttccgg
    12841 tgacagggaa gaaatctacg aaaacctgaa ggcatgccag tggaatccgt attccgtgag
    12901 ctggaataca aaattaaaaa agaattggta ggtatagctt atgaaatggg gaattttagc
    12961 aacaggaacc attgcagaga aatttgcaga tacagtaaac catatggagc aggaagatga
    13021 gactttgatc gcctgtgctt cccgcaattt agagcatgga aaagcatttg cttccaaata
    13081 cgggatcccg aaggtttacg gaagttatgc acagatgatg acagacagcg aagtagatgc
    13141 ggtttatatt gcaacgccaa acagtctcca ttatgaaaat tgccgtatgt gcctggaagc
    13201 aggaaaacat gtactatgcg aaaagccatt taccctttgt gtggatcagg cagaagaact
    13261 gtacgaactg gcagaggaaa aaggcctgtt tattatggaa gctttctgga tccggttttt
    13321 gccggcatat gataagttgc gggccatgtt aagagatggc gtgatcggag aggtaaagcg
    13381 gatcacttcc cagtttggat ttacagtgga aggcgccaga agagaacgca agtttaagtc
    13441 tgaattaggc ggcggagcgt tattagacat tggtatctac aatcttggat tttttcatat
    13501 gatcacagaa gctgcgccgg aaggatttca gtcagaagta catatgacag aatatggtac
    13561 cgatgattac agcgaagtag aactggaata tccgggaggc tgcagcgctc attgcatcca
    13621 ggcgatcgga aagaaattag aacgccacgc aaagatcgaa ggaacaaaag gtgatatcct
    13681 gatggatgat ttccagcatc tgcaggaatt taccgttcag ttaaaagatg gatcctctta
    13741 tcttgtaaaa gagccgttta aggtaaatgg ttttgaatat gagatcgtag agacaagccg
    13801 ctgtgtcagc tgtggtttaa ataccagtga ccgttataca aaagaagatt gcctgacgat
    13861 cctgcggtta atggatgata tcagggagtc atgggatatg acctttgaag gtgaagaaaa
    13921 ataaaataga gaaggttagt ttcaaggcgg gatttcagag acgggatccc gtcttttttg
    13981 tattttccag aattattttc tggaaaaaca aagggagtca tgtggtgtac aatataaggc
    14041 aaagaagaga tcagtaaagg ggatgaaaaa aatgacaatt tttgagtatt tatatgggga
    14101 cgtatattat acagatgaat atggaaacgc ttattttgat tcctgtgtgg atattgatta
    14161 tgaattagat gaaataccga aaattgtaaa tttaggggat catacctatt tcatggcgaa
    14221 ggaggattta gatctctata accagtatga tattaaggtg gatggggtta gtgaggatga
    14281 tctgaggttc ttacattata ccagaagacc ctattaccag atgcggggac gttccgtttc
    14341 cagagagcag gcatttgata tcatccgacg gacggacaat ttctttaact gggacatgga
    14401 aacaatagga aatcgcaaag agtttgtacg ttgcattaat tttgataact ggctcattat
    14461 gaaaaatcat tatcccaaag gatacggctg gatccacgcg gatggcacag tcggggccaa
    14521 tgcgatcact cagaagtggc cgacgatgat agagcttgta acagaatggt tttacaagtt
    14581 aaaatcattt ccttatctgg accttgtcat cgggattaca aattgggatg agatctcctg
    14641 ggatgaagat gacacttttg aaaaagcgat ccagatgggg atttatgtac atgataagtg
    14701 cattgagctc ttaaacaagc agaatgcctg ggcgaagtac caggaatacg atgagaaata
    14761 cggtgcagat ccggaacgat ttgaaacgga ttactatcag aaaaacggga tcgttcaggt
    14821 agatgaagcc tatctgagaa agtgcattga gtcttatggt ttggatccgg atgaggaatt
    14881 gagtaaggtt cggccttata tatggaaggg agaaataatg gaagaaaaca aaatgatcta
    14941 caaaaatgaa gatggcaatt atcacatcag gatcttaatg tcacaggagc agtgcaaaca
    15001 attaaagtta gcatatcccc tggaagtgga tgtatgtgtg tgtgaagcag aacgaaggaa
    15061 agagtgggag gaaagtatcc gggaaaagat cgaagatgct ttatatgaga tggtcgctcc
    15121 tgtggatgag gatgatttct attcggatgt atacagagca tggtttgagg aaattgcgtg
    15181 gaactgggag ctggaaaaag aagggttcct gccggaagaa aaggatcctt tctctgaaat
    15241 aaatatggat aggactttac tggcctatcg ccgtttgtgt gcctgcctga ctatagtggg
    15301 gaaacaaaag gaaatagagc gtgtttttct tctatcccgc cgacttcacc tttgtgtgcc
    15361 ccacgga
//
LOCUS       DBMDUA010000050        15256 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2154_length_15256_cov_2.379941, whole genome shotgun sequence.
ACCESSION   DBMDUA010000050 DBMDUA010000000
VERSION     DBMDUA010000050.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 15256)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15256)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15256
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2154_length_15256_cov_2.379941"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="ACZPCL_05680"
     CDS             <1..973
                     /locus_tag="ACZPCL_05680"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR1162000.1"
                     /translation="KFTEMFGIEKPIVQGGMQHLGIAEFASLVCNAGGMGTINITCYP
                     GVDEFHEDVIKMKEFTNNKPFIVNISLVPDLTKGEEIFKYIDVCAKEEVAAIEFAGAS
                     PVEFMPACKEAGIKIIHKSPNAKVAASMARKGADVITIAGYEVAGHPSMDGIGTFVIA
                     NKAAKVCAEYGVPVLAAGGVADGKGLAAALALGAQGVVLGTRFVATTECPISDNHKEW
                     LLEHTEKDTVIIQKSIHNAARVANNMAAKLTLEMEKRGTTLEELMTVIAGRLSKKCYK
                     NGDIDGGIYALGPAMGLIDDIKPVQQLMDDMVAEAEEVIGGLKASIY"
     gene            1029..2303
                     /locus_tag="ACZPCL_05685"
     CDS             1029..2303
                     /locus_tag="ACZPCL_05685"
                     /inference="COORDINATES: protein motif:HMM:NF014564.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR1162001.1"
                     /translation="MADETNYRPLEGVKVLELSTMVAAGSCGRMLADWGATVIKVEAE
                     KGDMFRNFPKTFLVPCTMDENPLFDNLNAGKRGIVLNLKIPEGMEAMHRLLAEADVFL
                     TNTRVKALKKLGLDYDSLKDKYPRLIEANISGFGEKGPKKDNPGFDTVAFWASSGFNA
                     DMMVEGPGSYPVYSSAGPGDIVTAMGLCYAITAALYKRTKTGKGDRVSSSLYGTALWC
                     FHIMAVATEERYGYQYPKTREVSAPTGAPFRTKDNQWVMTTILKIEEQWPVLCNVLGV
                     PELGTDPRYNTSLRQRDPEVRKYLMKRFEEIYATKTADEWCKLLTEADIVNDKLAHYK
                     DMEHSEQAWENEYIHEVTCPNGGKSILVRPAMRSDRMGIPTWKRGPMLGEHTEEVLKE
                     IGYTDEQIKAMEEKKAAVQIDTTQFKHLDEEM"
     gene            2316..3833
                     /locus_tag="ACZPCL_05690"
     CDS             2316..3833
                     /locus_tag="ACZPCL_05690"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR1162002.1"
                     /translation="MMEGQFHSLIDIFKTGKEQYPEKTAIIMGEDTRTYGQIFDNAKR
                     IAGFVLEKEIKPQDKVAVISQNHVDYIETVLGIIMAGGVPVNINWRLAAPELHHLLQF
                     NEVKLTFFRSKDESIRKEMGELTKEMCQCVDMDCIKEVLEGVEPLEQFIEREDEDVLM
                     HLHTSGTTGMPKTVRYTKGQYMRELTTCVHSLGFDKDTVFQLMSQLFHSASIGPYSIF
                     ACGGTIVLFPKFSPQEYLKSIEKHHVTRLSAIPTVLTALLNQPDFDRYDLSSIHTISY
                     STCPMPPELIKRALKHFNCGFQQSYGMTEMGSIVTMLTEEEHIKDNMRYLNSVGRPIE
                     DHKVKIIADDGSECAPGETGEIVVTGPGMMKDYYKMPDETKEVMVDGWYHTRDMGYLD
                     ENGYLYISGRKSDMIISGGENIFPLEVENVLRMREDIAEVCVLGVPDDYWGESVQAVV
                     VLRPGSKLTPEEIRTFCRGKIAGYKIPKKVYIWEELPKNTTGKVCKAEVLKKIEM"
     gene            complement(3895..5451)
                     /locus_tag="ACZPCL_05695"
     CDS             complement(3895..5451)
                     /locus_tag="ACZPCL_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1162003.1"
                     /translation="MFQAILCDDNEIILEGLSSQIKWKDLGIELTATAADGNEAWELI
                     QKNPPDLLITDIRMPYMDGLELSKKARELNPNIVILIISAYDDFQYARTAMHLQAFDY
                     ILKPIDVDQFCRVLENAVLHCSQLHYDKRLMITEYLRSIVTQDASDLPAPSPDLDLDP
                     GSYCCALHITMDEESLKRRSNDIRYEEEQNFSAFSTSLCQPGCYLLDSRHNSYTLALL
                     SGSKLELITLRKILIDSVRKIFPYDGQNCDVIIASGNLCHNLNRLNESMKSVAEALKL
                     HFIKGTNSDIFYEEVVFYIGKEADPSPKLPLSGTTLISLIKKQDKKGLERELLQLKEN
                     LRQCGRESYLYMTFSISRLYTDLASELGSSGIELQDVFDDPMEEFKKVASSTTLEESI
                     ENLKQNLFKIVDSLNARKSKYGKQVEQAIDHIKHHYSESTLSIEDVARTVCLSTSYFS
                     TIFKNETGITFTDYLIKIRMERAKNLLENTHMKMYEISSMTGYENAAYFSAAFKRYFG
                     KSPSDVQNHR"
     gene            complement(5429..7234)
                     /locus_tag="ACZPCL_05700"
     CDS             complement(5429..7234)
                     /locus_tag="ACZPCL_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HIR1162004.1"
                     /translation="MNDFHIKLSFRKKLLLSFIVLSFIPVLLIGITTYRLYNRFITDM
                     TEKSSVETIDLICNDIDSLLNDTWQLCDALTRDIKIQENLRVKFNSVSSQYSLDLSGS
                     MDMASLSTYRKDIFGVYVLGENGGRYKSNYYSFKPDDPRDSKWYQKIKNSPESIWFSP
                     HEGSFIVRSSIEDRFISVGLPSSDKATGRINGVICADIKEDDLTKKIQDSLNSGAVYL
                     LNAQGNILFQSGNNSHFPVSVSDELTGQILQAAKDADTRSVVIPNREYLVVGRTLNRS
                     GWCIAGVISRRYLAQSTKELTTILLLVLLIIAFSALYISLRISQSVYRPIQTLCTMME
                     AVEAGDFSVRYSEPPADELGRLGNNFNHMLNKIQELISQIYEEQKKLRNSEFKALQAQ
                     IQPHFLYNSLDSVIWFLRMGKNKDAEKMLTELSTLFKISLSKGREIITIEDELKHIGS
                     YLFISNMIYSKKFTYSIECDPSLYQYQTPKLLLQPLAENAILHAVPLPDEKVHIHVSI
                     RENGDCLLLSVQDISQGMSPETLEILRKQINAPRQPDKQDSGYGLYNVNERIRIFYGP
                     DFGLNIDSDPDIGTEITLKIPKLERGKPYVPSNPV"
     gene            complement(7267..8265)
                     /locus_tag="ACZPCL_05705"
     CDS             complement(7267..8265)
                     /locus_tag="ACZPCL_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1162005.1"
                     /translation="MTFLKKNWCLMIIAVLLFLLVYTIYTSSIPLPDVVNHVDDHITV
                     GFSQIETDNPWRTTQINSFREAFGPSNIDFIYHEPEEYTAKWQQEDITELIREKVDYL
                     VIVPADLDALVPALQSAKEAGIPVILIEQSADTIDPDLYMSRISADYLREGAICAQLL
                     AEKYAGRSCNIVEIYGTESSPSAKARSKGFHQALKNYPQLHIIDTEYGNFDRVTAQKA
                     MENALVKTSDSHKSVQAVFAHSDEDGLGALQAIKTAGLSPNDISIVSINGIQDVCKAI
                     IAGEYLGTVESNPKWGFIVETLINQRERGYTPFPTVIIPYNIITEANAVSYFPTAY"
     gene            complement(8290..8958)
                     /locus_tag="ACZPCL_05710"
     CDS             complement(8290..8958)
                     /locus_tag="ACZPCL_05710"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR1162006.1"
                     /translation="MFKILSYPIKDGDPTWPGNPTCSLEPHTSIAKGDTANTATIHLF
                     NHYGTHLDGPMHFNPNGISLDKVPVERFFYQKPLVIDVPKKAGEKILIEDLMPYKDQI
                     AQADFLFLRTGFWKKRKSDPSVYENNGPAVGSAAATYLRENFPNLKAIGLDFVSLASY
                     SDGKDGDLAHQIMLGMYQPGYICIVEDINMEELPEGFLKQAAAVPLIIVGIDSCPVTM
                     WAEF"
     gene            9167..10066
                     /locus_tag="ACZPCL_05715"
     CDS             9167..10066
                     /locus_tag="ACZPCL_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1162007.1"
                     /translation="MKVIDIHAHIYEKVAGITQGQPMSSTSQGKVTIGNREIQFLPPS
                     FERSCSTAEALIAYMDWCGIEKALLMPNPFYGYHNYYFKESVKKYPDRLRGVALVDIM
                     KGKAAAEELAQIYDEGILFGMKIEVDSTFQCAPGTRLADPKLAPVWDCCEQYHQPMFI
                     HMFRREDIVDLELLAKTYPHITFVICHMGADICFRAGMPKRNYEKVLQIVKEYENVYI
                     DTSTVPDYYEEEYPWKTSVNIIEECYRKVGADKMMWASDYPGMLKKGTLNQLIRLVTE
                     ECKNIPESAKEKIMAENARRLFF"
     gene            10149..11261
                     /locus_tag="ACZPCL_05720"
     CDS             10149..11261
                     /locus_tag="ACZPCL_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1162008.1"
                     /translation="MRKKVVAMGLAAMMAMSLAACSSGGSSTSTTAAGSSAEATTAAA
                     AASSEESKKEEAKGDGKGYVVALCNYSIGNSWRAQMEQEFVSEAEKLKSEGVVSEYYI
                     TNSNEDINKQISDMQDLITKKVDAIVITAASPTALAPVVEEATEAGIKVVSFDNVVDT
                     DEQVATVGIDETEFGRIGAEWLAEKLDGKGKIIVLNGIAGAATDSLRWGGAEEVFKNY
                     PDIEVLGSANASWDYAQGKAAVESMLSAYPEIDGVWSQGGAMTQGAIDAFIAAGRDLV
                     PMTSEGNNGALKAWYENRDNGLSCIAPSNPTYTSAEALRTVIKALDGEDVPKNVVMDI
                     ETVTEDNLEDYYRPDMPDSFWVLTELDDEALQKLYK"
     gene            11379..12908
                     /locus_tag="ACZPCL_05725"
     CDS             11379..12908
                     /locus_tag="ACZPCL_05725"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1162009.1"
                     /translation="MAEEILRMTNITKRFAGITALDRVEFSCNKGEVHILAGENGAGK
                     STILKILAGIHQADEGEIYFHGKKVTIKSPEHSQKMGIAMVFQELTLVGEMTVAENVY
                     LNQEPVNALGMIDKKKIHEEIEKAMDKYGIHVDPDALVSTLPVAKQQMVEILKILVRD
                     PELIILDEPTSALAKKEVVQLYQIIHNLLDNGKTIIFISHRLEELFELGDRITVFKDG
                     HYVGTRDMKEMNEDELIKMMVGRSLTNIFPPPVCEPDYSKVVFEAKNLTDNDGKLKDV
                     SFQVFKGEVLGIAGLQGHGQTELLNAISGLYPLTKGEIIVNGKTEKIKSASQAIQCGV
                     ALVPADRKRQGLMLELSNRHNLAVSSMKKRMNGPFIDQKAETSFAEDMVKRLSIKLGG
                     LELPVSSLSGGNQQKIVLGKELATEPKVILFDEPTRGIDVEAKREFYQIMHELAAQGV
                     AVIMNSSDMMEVIGMSSRVIVMYEGRISGTLQKEELSEELIMQFGMGMKKERTKEETE
                     G"
     gene            12905..13855
                     /locus_tag="ACZPCL_05730"
     CDS             12905..13855
                     /locus_tag="ACZPCL_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1162010.1"
                     /translation="MKNKLNKNFWNKHGHVVIIYVFLVALMLLVSVFSRDFFTIGNFK
                     NLLRTSFPLLMVALGQTLIILTGGIDLSLGGIVALANVVCVMTMNTESPVGFILPLIA
                     AVVLGLVCGALNGVLVTRGNLAPIIVTIATTAIFDGCALLLMPKPGGSVHKAFSKFLT
                     RGLKGAVPLILFLVILILVRTLTNQTPYGKALRAIGGSENAAYSTGVKVKKVKFIAYC
                     LAGLLCAAAGIFLSAQMNSADATIGKNYAMNAITATVVGGTAMTGAVGDPLGTIAGVF
                     IISIINNMLNLFGVSSFYQFVCQGLILILALSLSAMHKKH"
     gene            13880..14827
                     /locus_tag="ACZPCL_05735"
     CDS             13880..14827
                     /locus_tag="ACZPCL_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1162011.1"
                     /translation="MKKLCSKYLENSQIVVYLILILLLVLAQILSPGYLNPTHMAGIL
                     RLASFMGIAAIGQNLTILTGGIDLSIANTITFANVIAAQIMMGRDSSMLPALLAVIVM
                     GIVVGLINGTGVYWLKIPPFIMTLGVGTVIQGIFLIYTKGAPKGNAAPLLRSICGQSM
                     YGIVSGIVLIWAVLAVIAIVLLHKTPYGRKLYSVGINEEASRFSGINTGRVTFSVYLI
                     SAVIAAVSGFLLVGYTGTSFLDVGTSYNTKTIAAVVIGGTAITGGKGGYLGTIAGAVI
                     MTILDDFLTIVSIPDAGRQVVQGIIIILLVLVYSREKKK"
     gene            15043..>15256
                     /locus_tag="ACZPCL_05740"
     CDS             15043..>15256
                     /locus_tag="ACZPCL_05740"
                     /inference="COORDINATES: protein motif:HMM:NF037707.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1162012.1"
                     /translation="MKERTKILNHMIAKTDRTDNELWLPLWMHSYDTAGVMEYLYHNW
                     VPPAAIKVICKDMGEEMGLKVCLFLAY"
BASE COUNT         4500 a         2924 c         3765 g         4067 t
ORIGIN      
        1 aaaattcaca gaaatgtttg gaattgagaa accaatcgtc cagggcggta tgcagcattt
       61 aggaattgct gaatttgctt ccttagtctg caatgcaggc ggtatgggaa ccattaacat
      121 tacctgttat ccaggtgtag acgaattcca cgaagatgtg atcaagatga aagaatttac
      181 taataataag cctttcatcg taaatatttc cctggttccg gatctgacaa agggtgaaga
      241 aatctttaaa tatattgatg tatgtgcaaa agaagaggtt gcagccattg agtttgcagg
      301 tgcttcccct gtggaattta tgccggcctg caaagaagca ggtattaaga tcatccacaa
      361 gagcccaaat gcaaaggtgg cagccagcat ggcaagaaag ggcgctgatg tgattaccat
      421 tgcaggttat gaggtagcag gacatccaag tatggatgga atcggtactt tcgttattgc
      481 aaataaggca gctaaggttt gtgcagaata cggcgtacct gtactggcag ctggtggcgt
      541 tgcagacgga aaaggcttgg cagcagcact ggcattaggc gcacagggcg ttgttctggg
      601 aacacgtttt gtagcaacca cggagtgccc gatctctgat aaccacaagg aatggctgtt
      661 agagcataca gaaaaagata cagttattat ccagaaatcc attcacaacg cagcccgcgt
      721 tgcaaacaac atggcagcaa agctgactct ggaaatggaa aagagaggaa ccactctgga
      781 agaattgatg acggttattg caggaagact gagcaagaaa tgctataaga atggcgatat
      841 tgacggtggt atttatgcat taggaccggc aatgggcctg atcgatgata ttaagccggt
      901 acagcagtta atggatgaca tggtagcaga agcagaagag gtgatcggcg gattaaaggc
      961 ttctatttat taatgatcaa aagggataga agagaaggca ataaatacag aagggactaa
     1021 aaataataat ggcagacgag acaaattaca gacctttgga aggggttaaa gtactggaac
     1081 tttctacaat ggtggcagct ggctcctgtg gaagaatgtt agcagactgg ggtgctacag
     1141 ttattaaagt agaagcggaa aaaggcgata tgttccgcaa cttcccaaaa acattcctgg
     1201 taccttgtac gatggacgag aaccccttat ttgacaacct gaatgcagga aaaagaggta
     1261 tcgtattaaa cttaaaaata ccagaaggaa tggaagccat gcatcgcctt ttagctgagg
     1321 cagatgtatt cctgaccaat acccgtgtaa aagcattaaa gaaactggga ctggattacg
     1381 actccttaaa ggataaatat ccgagactga tcgaggcgaa tatttccggt tttggtgaaa
     1441 agggaccgaa gaaagataat cctggatttg ataccgttgc attctgggca agcagtggtt
     1501 ttaacgcaga tatgatggta gaaggacctg gaagttatcc tgtatacagt tctgctggtc
     1561 ctggtgatat cgtaactgca atgggtctgt gctatgccat tacagccgct ctttataagc
     1621 gcaccaagac aggaaagggc gaccgtgtaa gctcttctct ttacggaact gcactctggt
     1681 gcttccatat catggcagtt gctactgaag aacgttatgg ataccagtat ccaaagacca
     1741 gagaggtaag cgcacctaca ggcgctcctt tcaggacaaa ggacaaccag tgggtcatga
     1801 ctactatttt aaagatcgaa gagcagtggc cggtgctgtg taatgtatta ggtgttccgg
     1861 agctgggaac agacccaaga tataacacct ccctgcgtca gagagatccg gaagtgcgta
     1921 aatatctgat gaaacgtttt gaagaaatct atgcaaccaa gactgcagat gagtggtgca
     1981 agctgcttac agaggcagat attgtaaatg ataagctggc tcattacaaa gatatggagc
     2041 acagtgagca ggcatgggaa aatgaatata tccatgaagt aacctgtcca aatggcggaa
     2101 aatccattct ggtgcgcccg gctatgcgaa gtgaccgtat gggtatcccg acctggaagc
     2161 ggggccctat gttaggcgag cacacagaag aggttttaaa agagatcggt tatacagatg
     2221 aacagatcaa ggccatggaa gaaaagaagg cagctgttca gatcgatacc acccagttta
     2281 aacatctgga tgaggaaatg taaagaaggg gataaatgat ggaagggcag tttcattcct
     2341 tgatcgatat atttaaaaca ggaaaagagc agtatccgga aaagaccgcc attatcatgg
     2401 gagaagatac aaggacatat ggccagatct ttgacaatgc aaaaagaatt gccggttttg
     2461 tattggaaaa agagataaaa cctcaggata aagtggcagt gatctcccag aaccatgtag
     2521 actatattga gactgttctg ggtatcatta tggcaggcgg ggtgccagta aatatcaact
     2581 ggcgccttgc tgctccggag ctgcaccacc ttctgcagtt taacgaagta aaattgacct
     2641 ttttccgttc caaagatgag agtattcgaa aggaaatggg agagctgacg aaggaaatgt
     2701 gtcagtgtgt ggatatggac tgtatcaaag aggttcttga aggtgtagag cctttagaac
     2761 agtttattga gcgtgaggat gaagatgttt taatgcatct gcatacaagc ggtactaccg
     2821 gtatgccaaa gactgttcgg tatacaaagg gacagtatat gcgggaactg actacctgtg
     2881 tacattccct tggctttgac aaagatacgg tgttccaact gatgagccag ttattccatt
     2941 cagcaagtat tggtccttat tccatttttg cctgcggcgg aaccattgtg ctgttcccga
     3001 agttttcacc acaggaatat ttaaagagca ttgaaaagca tcatgttacc aggttaagcg
     3061 ctattcctac tgtactgact gctttgctta accagccgga ttttgaccgc tatgacctga
     3121 gcagtatcca taccatcagt tattccacct gtcccatgcc gccggagctg atcaaacgtg
     3181 cgttaaagca ttttaactgc ggcttccagc agtcctatgg catgacagaa atgggttcta
     3241 ttgtcactat gcttacagaa gaagagcata ttaaggacaa tatgcgatac ctgaattctg
     3301 taggacgtcc cattgaagac cataaagtaa agatcatcgc agatgatggt tctgaatgtg
     3361 ctcccggaga aaccggcgaa attgtagtga caggtcctgg catgatgaaa gattattata
     3421 aaatgccgga tgagacaaag gaagttatgg tagatggctg gtatcatacc cgtgatatgg
     3481 gctatctgga tgagaacggt tatctttata tcagcggacg caagagcgat atgatcatat
     3541 ccggcggtga aaatattttc ccgctggaag tagaaaatgt tctgcggatg agagaagaca
     3601 ttgcggaagt ttgtgttctt ggagttccgg atgactactg gggtgaatct gtccaggcag
     3661 ttgtagtatt aagaccgggc agcaaactga caccggaaga gatccgaacc ttttgcagag
     3721 gtaagatcgc cggttataaa ataccaaaga aagtatatat ctgggaagaa ctgccgaaaa
     3781 ataccacagg aaaggtttgt aaggcagaag tgcttaagaa gatagaaatg taatgtgtgc
     3841 ataataaaag tgctgcggtt ttcatagtcg cagcactttt taatgtctgt tctatcatcg
     3901 gtgattttgc acatccgacg gtgatttccc aaaatacctt ttaaaagcag cgctaaaata
     3961 agcggcattt tcataaccgg tcatagagga gatctcgtac attttcatgt gtgtgttttc
     4021 caacaggttt ttcgcccgtt ccatgcggat cttgatcagg taatctgtga aagtgatccc
     4081 ggtttcattt ttaaagatgg tgctgaaata ggaagtgctt aagcatacgg tccgggctac
     4141 atcttcaatg gaaagagtag actcggaata atgatgttta atatggtcga tagcctgttc
     4201 cacctgcttg ccgtatttgc tttttcttgc atttaaactg tcaacaatct taaaaagatt
     4261 ctgtttcagg ttttcaatac tttcctctaa ggtggtgctg gaagctactt ttttaaattc
     4321 ttccatagga tcatcaaata catcctgaag ttcaattccc gatgagccaa gttcacttgc
     4381 aaggtctgta tacagacggc tgatggaaaa ggtcatatac aggtagcttt ccctgccgca
     4441 ctggcgaagg ttttctttca gctgtaaaag ttcccgttcc agtccctttt tatcctgttt
     4501 tttgatcagg gagatcaggg tggtgccgga aaggggaagc ttgggagaag gatcagcctc
     4561 ttttccaata tagaaaacta cttcctcata gaaaatatca gagttggtgc ctttgataaa
     4621 atgcagcttt aatgcttctg ccacgctttt catactttcg tttaaacggt taagattgtg
     4681 gcacaggttt ccggaggcaa tgatcacatc acagttctga ccgtcgtaag gaaagatctt
     4741 ccggacagaa tcaatgagta ttttcctcaa agtgatcagc tccagcttgg aaccggataa
     4801 cagtgccaga gtgtagctgt tgtgcctgga atctaaaagg taacatccag gctggcataa
     4861 tgatgtactg aaggctgaaa aattctgttc ttcttcatag cggatgtcgt tagaacgtct
     4921 tttcaggctt tcttcgtcca tggtaatatg taaagcacag cagtaggagc cgggatccag
     4981 atctaggtca ggacttggtg caggaaggtc agaagcatcc tgcgtgacaa tgcttcgcag
     5041 gtattctgtg atcatgagac gtttgtcata atgaagctgg ctgcagtgaa gaactgcatt
     5101 ttcaaggaca cggcagaact ggtctacatc aataggcttt aaaatgtagt caaatgcctg
     5161 caggtgcatg gcagtgcggg catactggaa atcatcgtaa gcgctgatga tcaggatcac
     5221 tatgttggga tttagttccc gggccttttt ggaaagttcc agaccatcca tataaggcat
     5281 acggatatcg gtgatcagaa ggtccggcgg gtttttctgg atcagttccc aggcttcatt
     5341 tccatcggca gcagtagctg tcagttcaat acctaagtcc ttccatttga tctgactgga
     5401 aagtccttcc agtataattt cattgtcatc acacaggatt gcttggaaca taaggtttac
     5461 ctctctcaag ttttggtatt ttcagtgtaa tttcggtgcc tatgtcagga tcggaatcaa
     5521 tgttcagacc gaaatcgggg ccatagaaaa tgcggatcct ttcatttaca ttgtaaagtc
     5581 cgtatccgga gtcctgcttg tccggctgac ggggagcgtt tatttgcttt cgcagtattt
     5641 ccagcgtttc aggggacatg ccctgactga tatcctgtac agaaagaagc aggcagtctc
     5701 cattttcacg gatgctcaca tgaatatgga ccttttcatc tggaagggga acggcatgta
     5761 aaatggcgtt ttctgccaga ggctgcagta acagtttggg ggtctggtac tgatacaggg
     5821 acgggtcaca ttcaatggaa taagtgaatt ttttgctgta gatcatattg gagataaaaa
     5881 ggtaacttcc aatatgcttt aattcatctt cgatggtgat gatctcccgt cctttactca
     5941 gggagatctt aaataatgtg gacagctccg ttaacatttt ctctgcgtct ttatttttcc
     6001 ccatacgcag aaaccagatt acagagtcca gggaattgta gagaaaatga ggctgtatct
     6061 gtgcctgtaa tgccttaaat tccgaatttc gcagtttttt ctgttcttca tagatctggc
     6121 tgatcagttc ctggatctta tttaacatgt ggttaaaatt gttacccagc cgtccaagtt
     6181 catctgcggg aggctccgaa taacgaacgg aaaaatcccc ggcttctacc gcctccatca
     6241 tagtacagag agtctgaatg gggcggtata cagactggga gatacgcagg gaaatataca
     6301 gggcggaaaa tgcaatgatc aaaaggacca gcaaaaggat cgtggtcagc tctttggtac
     6361 tttgtgccag atatctgcgg ctgatcacac ctgcaataca ccagccggaa cgatttaatg
     6421 tgcggcctac taccagatat tccctgttgg gtatgaccac agaacgggta tctgcgtctt
     6481 tggcagcctg aaggatctga ccggttagtt catcagatac ggaaactgga aaatgggaat
     6541 tatttccaga ttggaaaagt atatttccct gagcatttaa aagatagaca gcaccgctgt
     6601 ttaaactgtc ctgtatcttt tttgtcaggt catcttcttt tatatccgca cagatcacgc
     6661 cgttgatccg tccggtagcc ttgtcagaag aaggaagtcc cacagagata aaacggtctt
     6721 caatggagct gcgcacaata aaactgcctt catgcgggga aaaccagatg gattcagggg
     6781 aatttttaat cttctggtac catttggagt ctctgggatc atccggtttg aaagagtaat
     6841 agttggattt atagcggccg ccattttcac ctaaaacata tactccgaaa atgtctttgc
     6901 ggtaggtgga aagggaagcc atatccatgc ttccggaaag atccagggaa tactggctgg
     6961 atacggagtt aaatttcaca cgcagatttt cctgtatctt aatatcacgg gtcagcgcat
     7021 cacatagctg ccaggtatca tttagcagac tgtcaatgtc attacagatc agatcaatgg
     7081 tctctacaga ggatttttcc gtcatgtcag taataaaacg gttgtagagc ctgtaagtag
     7141 tgataccgat cagaagcacc gggataaagg aaaggacaat gaaggaaagc aggagctttt
     7201 ttctgaaaga gagtttgatg tggaaatcat tcataataat gaaaacgcct ttcttttaat
     7261 atgccgttaa taagcagtag gaaaatagct gacggcattt gcttctgtaa taatattgta
     7321 agggatgatc accgtaggaa atggagtgta tccccgttcc cgctgattga tcagggtttc
     7381 tacaataaat ccccattttg gattgctttc caccgttcct aaatattccc ctgcaatgat
     7441 cgccttgcac acatcctgga tcccgttgat ggatacaata gaaatgtcat tgggagacag
     7501 accggctgtt ttgattgcct gaagggcacc aaggccgtct tcgtcgctgt gagcgaaaac
     7561 tgcctggacg cttttgtggg agtcactggt ttttaccagg gcattttcca tggctttctg
     7621 ggctgttaca cggtcaaaat ttccgtattc tgtgtcaatg atatgcagct gcggatagtt
     7681 tttcaaagcc tggtgaaagc ctttggagcg ggctttggca gagggagagc tttccgtacc
     7741 gtagatctct acaatgttgc aggagcggcc ggcatatttt tcagctagca gctgggcgca
     7801 gatcgctcct tcccgcagat aatccgctga gatccgggac atatacagat ccggatcaat
     7861 ggtatcggcg gactgttcaa taaggatgac cggtattccc gcttcttttg cgctttgaag
     7921 ggcaggaaca agggcatcca gatctgcggg aacgattacc agatagtcta ctttttcacg
     7981 gatcagttct gtaatgtctt cctgctgcca ttttgcggtg tattcttccg gctcatggta
     8041 gataaagtca atattactgg ggccaaaggc ttcacgaaaa ctgttgatct gggtagtccg
     8101 ccagggatta tctgtttcaa tctgtgaaaa tcctactgtt atgtgatcgt caacgtgatt
     8161 caccacatca ggcagcggaa tggagctggt gtaaatagtg tagactaaca ggaacaaaag
     8221 aacggcaatg atcatcagac accagttctt ttttagaaaa gtcataaata aacatcccag
     8281 agttattccc taaaactcgg cccacatggt gacaggacag ctgtcgatcc ctacaatgat
     8341 cagcgggaca gctgcagcct gttttaaaaa gccttccggc agttcttcca tgttgatatc
     8401 ttctacaatg cagatgtagc cgggctggta catgccaagc atgatctgat gggccagatc
     8461 gccgtctttt ccgtcactgt aagaagcaag ggaaacaaaa tccaggccaa tggctttcag
     8521 atttgggaaa ttttcacgta aataggttgc tgcagcgctt ccaacggccg gtccgttatt
     8581 ttcataaaca gaaggatcgc ttttgcgttt cttccagaaa ccggtgcgta aaaagagaaa
     8641 atctgcctgt gcgatctggt ccttgtaagg catcagatct tcgatcagga tcttttcacc
     8701 ggctttttta ggaacgtcga tcacaagagg tttctgatag aagaaacgtt ccacgggaac
     8761 tttgtccaga gaaataccat ttggattaaa gtgcataggg ccgtccagat gagtaccgta
     8821 gtgattaaaa agatggatag tagccgtatt tgcggtatct ccttttgcaa tggaagtatg
     8881 aggttccaga gaacaggttg ggtttcccgg ccaggtagga tctccgtctt taataggata
     8941 tgaaagaatt ttaaacataa aaatacctcc tgcttattca cgtattttaa tctgtaaaca
     9001 ttaaaacatg attttataag agcgtcaata aaatcaaatg aaatcaaaat attttcaaat
     9061 gaaatcacat atacagaaaa atatgtacag gctatactaa gaacagatca gaaaacacga
     9121 aaaaatgtac aaaaacacaa gagatgtgaa aagaaaggtc aatgttatga aagtcatcga
     9181 catacatgct cacatttatg aaaaggtagc tggtatcaca cagggacagc caatgtcatc
     9241 taccagccag ggaaaggtta cgatcggtaa cagggagatt cagtttcttc ccccttcctt
     9301 tgaacgcagc tgcagtacgg cagaagcgct gatcgcctat atggactggt gcggaattga
     9361 aaaagcgctg cttatgccaa atccatttta tggctatcac aattattatt ttaaagaaag
     9421 tgtaaaaaag tatccggacc gcttaagggg cgtagctctg gtggatatta tgaaaggtaa
     9481 ggcggcagca gaggaactgg ctcagatcta tgacgaaggc atcctttttg gtatgaagat
     9541 cgaagtagac agtacgtttc agtgcgcacc gggaacaagg cttgcagatc caaaacttgc
     9601 acctgtatgg gactgttgtg aacaatatca tcagccaatg ttcatccata tgttccgcag
     9661 ggaagatatt gtggatctgg aactgctggc aaagacctat ccccatatta catttgtaat
     9721 atgccatatg ggagcagata tctgcttcag ggcagggatg ccaaagagaa attatgaaaa
     9781 agtccttcag atcgtaaaag aatatgaaaa tgtttatatt gatacatcta cagttccgga
     9841 ctattatgag gaagagtatc catggaaaac atctgtgaac attatcgaag aatgttaccg
     9901 gaaggtaggc gctgataaga tgatgtgggc ttctgattat ccgggaatgt taaagaaagg
     9961 aactttaaat cagctgatcc gtctggtaac agaggagtgc aagaatattc cggaatcagc
    10021 caaggaaaag atcatggcag aaaatgccag aaggttgttt ttttaaaagc tgtttttaag
    10081 attgatatgg ttctgcaggc agcagaatga gataaaatca ttaatcacat caaaggagag
    10141 gtaacagtat gagaaaaaaa gtagtagcaa tggggttagc agcaatgatg gcaatgagtc
    10201 tggcggcttg cagctcagga ggatcttcca catcaactac agcagcagga agctcagcag
    10261 aggcaactac agcagctgcg gctgcatctt ctgaagaaag caaaaaagaa gaagcaaagg
    10321 gagatggtaa aggatatgta gtagcgcttt gcaactattc tatcggaaac tcatggcgtg
    10381 cccagatgga gcaggaattt gtatctgaag cagaaaaatt aaaatcagaa ggcgttgtaa
    10441 gtgaatatta tatcaccaac tctaatgaag atattaacaa gcagatcagt gatatgcagg
    10501 atctgatcac aaagaaggta gatgctattg taattactgc agcatctcca actgctctgg
    10561 cacctgttgt agaagaagca acagaagctg gtattaaggt tgtatctttt gataacgtag
    10621 ttgatacaga cgagcaggta gcaactgttg gtattgatga aacagaattt ggacgcattg
    10681 gtgctgagtg gctggctgaa aaactggatg gcaagggcaa gatcattgta ttaaatggta
    10741 ttgccggtgc agcaacagac tccttacgtt ggggcggtgc tgaagaagta tttaagaact
    10801 atccggatat tgaagtttta ggttctgcaa atgcatcctg ggactatgca cagggcaagg
    10861 cagctgttga gtccatgctt tccgcttatc ctgagattga tggtgtatgg tcacagggcg
    10921 gtgctatgac acagggagca attgatgctt tcatcgcagc aggacgtgat ctggttccta
    10981 tgaccagtga aggcaacaat ggtgcattaa aggcatggta tgaaaatcgc gataacggct
    11041 taagctgcat cgctccttct aacccaactt acaccagtgc agaagctctt cgtactgtaa
    11101 ttaaggcttt agatggtgaa gatgttccaa agaacgtagt tatggatatt gaaacagtta
    11161 ctgaggataa cctggaggat tactaccgcc cggatatgcc agacagcttc tgggtattaa
    11221 cagagctgga tgatgaagca cttcagaaac tttacaagta atccggaata aaagaaataa
    11281 acaaggggct gccgcaaaga aacttgcagc agccctttaa agaatatgtc ctcccggaat
    11341 cttgggagca tatggatatg ttgaggagga ggtctatcgt ggcagaagag atactgagaa
    11401 tgacaaatat tacaaagcgt tttgctggga tcaccgctct ggaccgggta gaattttcct
    11461 gtaataaagg tgaagtacac attcttgcag gtgaaaatgg tgccggaaag agtaccatat
    11521 taaagattct ggctggcatc caccaggcag atgagggtga gatttatttc catggcaaaa
    11581 aggttactat taaaagcccg gagcactccc agaaaatggg aattgcgatg gtattccagg
    11641 aactgacact tgtaggagag atgacagttg ctgaaaacgt atatctgaac caggagccgg
    11701 taaatgccct gggaatgata gacaaaaaga agatccatga agagatcgaa aaagcaatgg
    11761 ataagtatgg gatccatgta gatccggacg ctttagtcag cacacttcct gttgcaaaac
    11821 agcagatggt agagatttta aagatcctgg taagagatcc ggaactgatc atcttagatg
    11881 aacccacatc tgcgctggca aagaaagaag ttgtccagct gtatcagatc atccacaacc
    11941 tcctagataa cggtaagacc attattttca tttcccaccg tctggaagag ctgtttgaac
    12001 tgggtgaccg aattaccgta tttaaagacg gtcattatgt gggaaccagg gatatgaagg
    12061 aaatgaacga ggatgagctg atcaaaatga tggtaggccg ttctcttact aatattttcc
    12121 cgccgccagt ctgtgagccg gattacagca aagtagtatt tgaggcaaag aaccttacag
    12181 ataacgatgg aaaattaaaa gatgtaagtt tccaggtatt taaaggagaa gttcttggaa
    12241 ttgcaggtct tcagggacat ggtcagacgg agcttttaaa tgccatcagc ggactttatc
    12301 cgctgactaa gggagagatc atagtcaatg gaaagaccga aaagatcaaa agcgcaagcc
    12361 aggccatcca gtgcggcgta gccttagttc ctgcagaccg taagagacag ggacttatgc
    12421 tggagctttc caaccgtcac aatctggctg tttccagtat gaaaaaaaga atgaatggac
    12481 catttatcga tcagaaagca gaaaccagtt ttgcggaaga tatggtaaaa cgcctttcta
    12541 ttaaattagg tggcttggag cttccggttt ccagcctttc cggtggaaac cagcagaaaa
    12601 tagtacttgg aaaggaactt gcaacagagc ctaaggtgat cctgtttgat gaacctacca
    12661 gaggtattga tgtagaggca aagcgtgagt tttaccagat catgcatgaa ctggcagcac
    12721 agggagtagc cgtgatcatg aattccagcg acatgatgga agtgatcgga atgagcagcc
    12781 gtgttattgt tatgtatgaa ggcaggatct ccggtacatt acagaaagaa gagctgtcag
    12841 aagagctgat catgcagttt ggcatgggca tgaagaagga acggacaaag gaggagacag
    12901 aaggatgaag aacaagctaa ataaaaactt ctggaacaaa catggacatg tggtgatcat
    12961 atatgtattc ctggtcgcac ttatgctcct tgtaagtgtg ttcagccgtg actttttcac
    13021 tataggaaac tttaaaaacc tgctcaggac ttcttttcct cttttgatgg tagcattagg
    13081 acagacactg atcattttaa caggtggtat tgacctgtca ttaggcggta tcgtggcact
    13141 ggccaatgta gtctgtgtta tgactatgaa tacagaatct cctgttggat ttattttacc
    13201 gctgatcgca gcagtagtcc tgggattggt ttgcggtgct ttaaacggag tgctggtaac
    13261 aagaggaaat ctggctccta ttatcgtaac cattgcaaca acagcaattt ttgatggctg
    13321 cgcattgctt cttatgccaa agccgggcgg ttctgtacat aaggcatttt ctaaatttct
    13381 tacaagaggt ttaaagggag cggttccgct gatcttattc ctggtgatcc tgatccttgt
    13441 gagaacctta acaaaccaga ctccatatgg aaaagcttta agagctattg gcggcagtga
    13501 aaatgctgct tattccacag gtgtaaaggt aaaaaaggta aaattcattg cttactgtct
    13561 ggcaggtctt ctctgtgcag ctgccggtat cttcctcagt gcccagatga actctgcaga
    13621 cgcgacgatc ggaaagaact acgcaatgaa tgctattaca gcaactgtag taggcggaac
    13681 tgccatgacc ggtgcagtag gtgatcctct gggaaccata gcaggcgtat ttattatttc
    13741 cattatcaat aatatgctaa acctttttgg tgtatcgtca ttctaccagt ttgtatgtca
    13801 gggtctgatc ctgatcctgg cactgtcctt aagtgcaatg cataagaaac attaacagaa
    13861 gaaaggaagg tgtttaatcg tgaagaaact gtgcagtaaa tatttagaaa acagtcagat
    13921 cgtagtttac ctgatcctga tccttcttct ggtactggct cagatattat ctccgggata
    13981 tttgaatccc acccatatgg caggtatttt aagacttgcg tcctttatgg ggatcgcagc
    14041 aatcggacag aaccttacga tccttacagg aggtattgac ctgtctattg caaataccat
    14101 tacctttgca aatgtgatcg cagcccagat catgatggga cgtgacagtt ccatgcttcc
    14161 ggctctttta gctgtgatcg taatgggcat cgtagtaggc ctgatcaatg gaactggtgt
    14221 ttactggtta aagatcccgc catttattat gacactgggt gttggaactg tgatacaggg
    14281 tattttcctg atctatacaa agggtgcacc aaagggaaat gcagcaccat tgctccgttc
    14341 catctgcgga cagagtatgt atggtatcgt atccggtatt gttctgatct gggcagttct
    14401 tgcagtgatc gccattgtac tgttacataa gactccttac ggcagaaagc tgtattctgt
    14461 aggtatcaat gaagaagcat cccgcttctc cggtatcaac acaggccgtg ttactttctc
    14521 tgtttatctg atctctgcgg tgatcgcagc tgtatcaggc ttccttctgg ttggatatac
    14581 aggaaccagc ttcctggatg taggaaccag ctataataca aagaccattg cagcagttgt
    14641 catcggtgga acagccatta caggtggaaa aggcggctac ctgggaacca ttgcaggtgc
    14701 tgttatcatg actatcctgg atgatttctt aaccattgta agcatcccag atgcaggccg
    14761 tcaggttgta cagggcatta tcattatcct tttggttctg gtgtatagca gggaaaagaa
    14821 gaaatgatga atggttagat gtgggattaa tatgagataa aggtgagatg atattagcag
    14881 cgacgctgta tcggtgagag atcattggta tggcgttgct gttttttatg aaacaaaaaa
    14941 taaaactgat taaatactat tgaaataaag tgagcgttga cttttgagtg gaagagaggt
    15001 atattggagt cagatagtaa gagagacagg ggggaaaaag gagtgaaaga aagaacgaaa
    15061 attctgaatc atatgatcgc aaagacagac cgtacagata atgaactttg gcttccgtta
    15121 tggatgcatt cttatgatac ggctggcgtt atggaatacc tttatcataa ctgggttccg
    15181 ccagccgcta taaaagtgat ctgtaaagac atgggggaag agatgggatt gaaggtatgc
    15241 ctgtttcttg catatt
//
LOCUS       DBMDUA010000051        15145 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2166_length_15145_cov_4.165847, whole genome shotgun sequence.
ACCESSION   DBMDUA010000051 DBMDUA010000000
VERSION     DBMDUA010000051.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 15145)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15145)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15145
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2166_length_15145_cov_4.165847"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="ACZPCL_05745"
     CDS             <1..330
                     /locus_tag="ACZPCL_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1162013.1"
                     /translation="GEVIAPFDGEVTAAFPTGHAYGLKREDGLECLIHIGLDTVELNG
                     SGFDVKVKQGDQVKQGQVLVKVDLDQVTGAGKSLVSPVVFSSGAMFTLLEKEFAVKGE
                     EILRLEQ"
     gene            complement(424..2679)
                     /locus_tag="ACZPCL_05750"
     CDS             complement(424..2679)
                     /locus_tag="ACZPCL_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HIR1162014.1"
                     /translation="MSETKIIPTTGRNNCGGRCIIYAHVCDGVIEKLSTETKGTPEHP
                     VPLCACAKGLNYHKTFLGEDRLRWPMKRVGERGEGKFARISWEEALDILTSEYIRIRD
                     TYGPGSRYVNDGCGISAVMRGDRMMQRLLALDGGYLGCYNSYSSACIRNATDITYGTS
                     ETGTHPSDWLNSHLIILWGHNPAETKFDSSTMFYLKKAKAAGIPIIVIDPRKNDTAVA
                     LNAQWIPIRPATDSALADAMAYVIIKEGLQDQEFLDKCCLGFDAAHMPEGADPSLNCL
                     SYLMGETDSIPKTPEWGEKITGIPADTIRELAIRYATTKPAAIIQGYGAQRNAYGEQS
                     ARGAILLACLTGNVGISGGSAAGAGDCSTHELPGFPVLDNPYNRALPVFLWTDAVDHG
                     KGMNEYDGIRTCDQGIFDDPKDITLNSNIKMIFNLASNTLVNQHGDINRTAKLLKDTS
                     KCEFIVCSDLFMTASAKFADLLLPGVSMFEEENITKPWKFTEFLGFNNKVIEPLYECK
                     TEYEWIRELAKRIDLEEEFTEGRDYSQWMRYIYDDLRTREPELPEYDEFREKGIYKFE
                     EGHYPISFEKEVKDPEHHPFPTPSGKIELFSTKLWKTPMKDFMPPIPRYVAPPEGPED
                     PLTKRFPLQLSGWHSKCRTHTVHDNNLGLRRLDPQKLWIHPEDAAARSIHDGDMVLVY
                     NDRGQVKIPAKITDRVAKGVATLSQGTWYTPDENGVDTRGSINVLTSQRPTPFARGNG
                     QHTNLVEVKKL"
     gene            2904..3845
                     /locus_tag="ACZPCL_05755"
     CDS             2904..3845
                     /locus_tag="ACZPCL_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1162015.1"
                     /translation="MKKRWLSLAVAATAAITVLAGCGSKAAETTAAATEAATTEAASS
                     EAAESSAEETKAEAAAEGEIPAPEGDDNKISIGVTPVPHAEIVNDVVVPKLEAAGWDV
                     EVVEFNDYVQPNTSLEDGEIDANYFQTIRYLEEQNKERGLHLVEVAGIHLEPMGIYSK
                     NYKSLEELPDGATIAVPNDGSNESRAIKLLADNGLITLAETEDLYNLTSIAENPHNFE
                     ITELDAANLPRSLDDVDAAVINGNYALEANLNPEKDALAAELADSDESYKYINYLVVK
                     EGNEESTKTKALIAALQNDDVKNYIEEKYSGSVIPAF"
     gene            4038..5012
                     /locus_tag="ACZPCL_05760"
     CDS             4038..5012
                     /locus_tag="ACZPCL_05760"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HIR1162016.1"
                     /translation="MINIEHVSKQFGSLQVLEDISINIDQGDIFGIIGQSGAGKSTLL
                     RCINGLEKYNGGSITVEGKEIAGLSQKELQLVRKDMGMIFQNFNLLGRLNVYDNIALP
                     LQFWGQNKNSKENHDRIMELLDLVGLKDKVNSYPKQLSGGQKQRVAIARALVLNPKIL
                     LCDEATSALDPQITKGILALLLKINKELGITIVVVTHQMEVVKEICNKVAFLKHGKMI
                     ATGKPEELFVAPNKDVQEFVGDSVEFLPKDGVNVRLFFNDETASDSAITHLAHELGIN
                     FGICQGKLENFRGTIMGSLVINVTEADRERVTAWLTDRGILWEVLENV"
     gene            5005..5640
                     /locus_tag="ACZPCL_05765"
     CDS             5005..5640
                     /locus_tag="ACZPCL_05765"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HIR1162017.1"
                     /translation="MYDVIMKAFGQTVYMVIGSTIFSVILGFIPAIVLTVTAPDGLRP
                     NKIIYGILDILVNVFRSFPFIILMVIVIPFTRMIAGKAIGTKAALVPLTISAIPFVAR
                     VIESALRSVDHGLIEAARSFGAGDLQIIFRVYLKEALPAILSGITLTMISLIGYSAMG
                     GAIGAGGLGDVAIRYGYQAYKMPYLISTSIVLIIFVQVIQSVGNLLYKKLS"
     gene            complement(5795..8152)
                     /gene="feoB"
                     /locus_tag="ACZPCL_05770"
     CDS             complement(5795..8152)
                     /gene="feoB"
                     /locus_tag="ACZPCL_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR1162018.1"
                     /translation="MTLRDLPIGKTATILSVGGEGALRQHFLDMGLIPKGEVTMVKYA
                     PMGDPMELRIHSYELTLRLADAEKIEITDVRDGVLPRPQNPDLSTIEQSKPHPGLGEG
                     GKYHIKATENPLPDTELLTFALAGNQNCGKTTLFNQLTGSNQHVGNFPGVTVDRKDGM
                     IRGKSNTLVTDLPGIYSMSPYSSEELVTRNFLLDEHPKGIINIVDATNIERNLYLTMQ
                     LMELDIPMVLALNMMDEVRDNGGSIMVNQMEEMLGIPVIPISAAKNEGIDELVEHAIH
                     VAKYQEPPRTMDLCDVNDDGGAVHRCLHAIMHLIEDHAKAAGIPLRFAASKLAEGDSL
                     IINSLNLDENEKQTLEHIITQMEAERGLDRAAAIAHMRFDFIAKTCDASVIKPRESKE
                     HLRSVKMDKILTGKYTAIPCFAGIMGLVFYLTFGVIGSFLSDLLDSCISALSTLVDHF
                     LILWNVNPVIHSLVMDGIFNGVGSVLSFLPIIVTLFFFLSLLEDSGYMARVAFVMDKL
                     LRKIGLSGRSIVPMLIGFGCTVPGVMASRTLPSERDRKMTILLTPFMSCSAKLPIYAF
                     FTAAFFPEHGTLVMIALYFGGIIMGILMALILRGTLFNGEAVPFVMELPNYRMPGAKN
                     VGLLLWDKAKDFLQRAFTVIFMATLAIWFLQTFSLRLNIVTDSKDSILAMISGLIAPL
                     MHPMGLGDWRIATALITGFLAKESVVSTLSILFGKTSTLVSSMTSLSAASLLVFCLLY
                     TPCVAAIASIKRELGGKWALTVVILQCVVAWIAAYIVYLIGGFFF"
     gene            complement(8313..9848)
                     /gene="putP"
                     /locus_tag="ACZPCL_05775"
     CDS             complement(8313..9848)
                     /gene="putP"
                     /locus_tag="ACZPCL_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HIR1162019.1"
                     /translation="MISGQAGILAAIVIYLLAMVYVGFYFSKKGSGDSADDFYLGGRK
                     LGPFVTAMSAEASDMSSWLLMGLPGVAYLTGIADAGWTAIGLGIGTYLNWLIVAQRLR
                     RYSIVCNAITIPDFFSRRFKDDKNILMCISAIIILVFFIPYTASGFKAIGTLFSSLFG
                     TDYHIAMIVGAVVIVGYTVMGGFMAVSTTDLIQSVVMSISLVVIVFYGIHMAGGWSEV
                     VNNATSLAGYLSITQLHDRATASAAPYGLLSIVSTVAWGLGYFGMPHILLRFMAIEEE
                     KKLVLSRRIATVWVLISMCVAILIGIIGYGASVAGSIPMFTTSSQSETVIIQFAHLLG
                     QNGFLLSLVAGVVLAGILACTMSTADSQLLTAASGVSQNLLQDFLKIKMSTAASMNAA
                     RLTVMGIAIVAVFLAWNPDSSVFTIVSFAWAGFGASFGPLVLFALFWRRTNMQGALAG
                     MLTGGVMVFVWKYLVRPLGGNFNIYELLPAFVLSCLAIVIVSLLTKEPSKEIYDEFDS
                     VGK"
     gene            complement(10114..10698)
                     /locus_tag="ACZPCL_05780"
     CDS             complement(10114..10698)
                     /locus_tag="ACZPCL_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1162020.1"
                     /translation="MAKKSTRNTRGRIVSAAWKLFYEQGYEDTTVEEIIELSGTSKGS
                     FYHYFDGKDALLSTLSSLFDEKYEELTGLLTPEMTAMEQLLFLNRELFHMIEDSISLD
                     LLARLYSTQLTTNGEKHLLDHNRIYYKLLRKIIAKGQAEGQLSSRSNVNEMVKLYALC
                     ERALLYDWCLCAGEYALTEYSAKVLPGFLSSFLP"
     gene            complement(10805..12118)
                     /locus_tag="ACZPCL_05785"
     CDS             complement(10805..12118)
                     /locus_tag="ACZPCL_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR1162021.1"
                     /translation="MLSIIIILSIALAIFLGYRTKINTGLFCIVFAYIIGCFVMGLKP
                     KQIIGFWPTSTMFVILSVSLFYNFAAINGTLEKMSGALLYACRNFPGLLPYALFAVAV
                     ILSVMGATFFTVLAFLAPITLVICDESRMDKLTGAVAINCGALAGGNFPTSNLGVVFR
                     GLSDTAYEASPEIAAVDSFNMEMKIFAFAIFFSLILITIFRFGFKENRNIGKGVTFKK
                     PESFTKDQKTTLTLMMAMMAVVLIFPLLNILMPGNAAVKYISGKVDVGLVAIIFTVIA
                     LLLKLAPQKEAIAKVPWNTIIMIAGAGMLIAVAVEAGTIEALSTWIGSNVPKPLVPIA
                     FCLVGAIMSFFSSTTGVVAPALFPLIPNLAASVGLSAPVLFACTILGAQSSAISPFSS
                     GGSLILGSTPKEEERNELFNRLLMVAVPISVITCTIYNFVIAMVL"
     gene            complement(12175..13035)
                     /locus_tag="ACZPCL_05790"
     CDS             complement(12175..13035)
                     /locus_tag="ACZPCL_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HIR1162022.1"
                     /translation="MGKISMKGVCDMHVHTNPDLRLRAYDDFELADAAVRVGARAIVI
                     KTHLGFTVNRAYLTNQYVKKVYGENTGFTMYGGVVMNKVIGGINPEAVEKGLKLGAKE
                     IWLPTQSAKRHLEKMGQDPAKGIELVRDGKVVPELVDVFKLIRDYDVVLGTAHVSPEE
                     AFVVVEAAKDAGVKKIVITHPEWWVVDMSIDDQIRLVKDYDVILERCYAQNMGGGAYK
                     SNLPDNLELIKAVGYEHVMVDTDGGQTENPHWELALEEYMQYLADHGIPEEHIYHMTR
                     TIPYKLLGIE"
     gene            13223..14149
                     /locus_tag="ACZPCL_05795"
     CDS             13223..14149
                     /locus_tag="ACZPCL_05795"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1162023.1"
                     /translation="MEMKELDYFIAIAEEKSISKAAERLFMAQSSLSQFLSILENNVG
                     SKLFIRTSRGVRLTQEGELMLQYAYKTLAEYRSVQNEIKDMKQLKSGRVIMGVSSFRG
                     SYLLPPVLNAFRLEHPDVKVEIVEQNSMVLEQQLLSGEIDIALLVMSEADPRLNIEWV
                     LKDEICLITSHTYPVMEFARNNEDNPDSRIPQYVNLKDTLSYEYLLSDYDTILGREAR
                     QIFNKNGIQPIASNHKLSALMSAAMGAAGLGLAFTYYSSRHYFRNAEFLSLGAESAFI
                     DLGIALPPGRYHSKAALAFKDTLKEVLQAADG"
     gene            complement(14176..14457)
                     /gene="spoIIID"
                     /locus_tag="ACZPCL_05800"
     CDS             complement(14176..14457)
                     /gene="spoIIID"
                     /locus_tag="ACZPCL_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HIR1162024.1"
                     /translation="MKDYIEERAVAIANYIIDHNATVRQTAKRFGISKSTVHKDCTER
                     LISINPALAAQTRVVLDLNKSERHIRGGMATKEKYQYQHRLAICSREEK"
     gene            14645..>15145
                     /locus_tag="ACZPCL_05805"
     CDS             14645..>15145
                     /locus_tag="ACZPCL_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylate symporter family
                     transporter"
                     /protein_id="HIR1162025.1"
                     /translation="MKKIVQAWTGISLVQRIVAGLVIGALLGMFVPSEGGIAILGDVF
                     VGALKAIAPLLVFFLVISSLCYAGKSHGGVIRTVIIMYMFSTFLAAIVAVTASSVFPV
                     TLTLVDAATDMAAPQGVAEVLRTLLMNVVTNPVQSLVSANYIGILVWAVLIGIALRKA
                     SDTTKQS"
BASE COUNT         4342 a         3285 c         3600 g         3918 t
ORIGIN      
        1 ggtgaagtaa ttgctccatt tgacggcgaa gtgacggctg catttcctac aggacatgcc
       61 tatggtttaa aacgtgaaga cggtttagaa tgtttgatcc atattggttt ggatacagta
      121 gaattaaatg gttctggatt tgatgtaaaa gtaaaacagg gggatcaggt aaaacaggga
      181 caggtgctgg taaaggttga tctggaccag gtgacaggcg caggaaaatc tctggtatca
      241 ccggttgtat tttcaagcgg agcaatgttt acgctgttgg aaaaagagtt tgctgtgaaa
      301 ggtgaagaaa tcttacgtct tgaacagtaa ttataaaaca gataatattt attgagcact
      361 cagttaaaag caggagttgt ccgaactgat attttgcgga taactcctgt ttttcgtata
      421 ttgttataat tttttcactt ccaccaggtt cgtatgctgt ccgttccctc ttgcaaatgg
      481 agtcggtctc tgggaggtga gtacgttgat ggagcctcgt gtatctacgc cgttttcgtc
      541 tggcgtatac caggtgccct gggaaagagt ggcgacacct ttggctaccc ggtcggtgat
      601 cttagcaggg atctttactt gcccccggtc attgtataca agaaccatat caccgtcatg
      661 gatgctacgg gcggcggcat cttccgggtg gatccagagt ttttgtgggt ccagtctgcg
      721 aagccccagg ttattgtcat ggaccgtatg ggtgcggcat ttgctgtgcc agccggataa
      781 ctgcaacgga aagcgctttg tcagcgggtc ctccggacct tccggcgggg ctacgtaccg
      841 ggggattggc ggcataaagt ctttcatagg agttttccac aatttagtag aaaacagctc
      901 gatcttccca cttggagtcg ggaatggatg atgctccggg tcttttacct ctttttcaaa
      961 ggaaatagga taatgacctt cttcaaattt ataaataccc ttttcacgga attcatcata
     1021 ttccggcagt tccggttctc tggtgcgcag gtcatcgtaa atatagcgca tccactggct
     1081 gtaatcacgg ccttctgtaa attcttcttc taagtcgatc cgctttgcca gttcccggat
     1141 ccattcatat tctgttttac attcataaag aggttcaatg accttattgt taaatcccag
     1201 aaattccgta aatttccacg gctttgtaat attttcctcc tcaaacatgg aaactcctgg
     1261 aaggagcagg tctgcaaatt tggcgctggc ggtcataaat aagtcggagc agacgatgaa
     1321 ttcgcacttg gaagtgtctt ttaaaagttt ggcagtgcgg ttgatgtccc catgctggtt
     1381 tacaagggtg ttgcttgcca ggttgaagat catttttata ttggaattca gtgtaatatc
     1441 ctttggatca tcaaagatcc cctgatcgca ggtacggatg ccgtcatatt cgttcatgcc
     1501 tttaccatga tccactgcat cagtccagag gaaaactggc agtgctctgt tataaggatt
     1561 atcaaggacc ggaaatcccg gcagttcatg ggtggaacag tcaccggcac cggctgcaga
     1621 accaccgctg atgcctacat ttcctgtaag acaggcaaga aggatagcgc cacgggcact
     1681 ttgctcccca tatgcatttc tctgggcgcc atatccctgg ataatggcag ccggttttgt
     1741 ggtggcatag cggatcgcca gctcacggat ggtgtcagct gggataccgg tgatcttttc
     1801 accccattca ggtgtctttg gaatactgtc tgtttcgccc attaaatagg aaaggcagtt
     1861 aagggatgga tctgcgcctt ccggcatatg ggctgcgtca aagccaagac agcatttatc
     1921 taagaattcc tgatcctgaa gcccttcttt tataataacg taggccatgg catccgccaa
     1981 agcgctgtct gtagcaggac ggatggggat ccactgggca tttaaggcaa cagcagtatc
     2041 attttttcgg ggatcaatga caataatggg aataccggcg gcttttgcct tttttaagta
     2101 aaacatggtg ctgctgtcaa attttgtctc tgcaggatta tgaccccaca ggatgatcag
     2161 atggctgttt aaccagtcgg aaggatgggt gccggtctcg ctggttccat aggtaatatc
     2221 cgttgcattg cggatgcagg cagagctgta ggagttatag cagccaagat aaccgccgtc
     2281 taatgccagc aggcgctgca tcatacggtc gccacgcata acagcgctga tgccgcagcc
     2341 gtcattgaca tagcgggaac cgggaccgta agtatcccgg atgcggatgt attcagatgt
     2401 aaggatatcc agggcttcct cccagctgat ccgggcaaat ttcccttctc cacgttcacc
     2461 gacccgtttc ataggccagc gaagccggtc ttcgcctaaa aatgttttat ggtagtttaa
     2521 gcctttggcg caggcgcata agggaacagg atgctccggt gttccttttg tttcagtgga
     2581 aagtttttca ataactccgt cacagacgtg ggcgtagatg atgcagcggc cgccgcagtt
     2641 attccgcccg gttgtgggaa tgatctttgt ttcgctcatg gggggtatgc ctttctttta
     2701 taggtatatt tattagagta tagcataatc agaaattgcc tgttataggt tttacctata
     2761 gccagataaa gattttatga aatagacaga ccaaggggga tgtgttagaa ttcagactat
     2821 caaaagaaac acagacaaca agcaacaaac acaaacaagg gagatcagac caccggggcc
     2881 tgataagaga ggagacaaat attatgaaga aaagatggtt atcattagca gtagcagcaa
     2941 cagcagcaat taccgttctt gcaggatgcg gcagcaaggc agcagagact accgcagcag
     3001 caactgaggc agcaaccacc gaagcagctt cttctgaagc agctgaaagc agcgcagaag
     3061 agaccaaggc agaggcagca gctgaaggcg aaattcctgc tccggaaggc gatgacaaca
     3121 agatttctat cggtgttaca ccggttccac atgctgagat cgtaaatgat gtagtagttc
     3181 caaagctgga agcagctggc tgggatgtag aagtagttga atttaatgat tatgttcagc
     3241 caaataccag cctggaagat ggcgagatcg atgcaaacta tttccagact atccgttatc
     3301 tggaagagca gaacaaggaa agaggacttc atttagtaga agttgcaggc atccacttag
     3361 agccaatggg tatctattcc aagaattaca agagcctgga agagcttcct gacggagcaa
     3421 ctattgcagt accaaacgat ggatccaatg aatccagagc tattaagctg ttagcagaca
     3481 atggactgat cactctggct gaaacagagg atctttacaa cctgaccagc attgcagaaa
     3541 atccacataa tttcgagatc actgagctgg atgcagcaaa cctgccaaga tccctggacg
     3601 atgtagacgc agcagttatc aatggaaact acgcactgga agcaaactta aatcctgaga
     3661 aggatgcttt agcagcagag cttgcagaca gtgacgaaag ctacaagtac attaactatc
     3721 tggttgttaa agagggaaat gaagaaagca ctaagactaa ggctctgatc gcagcattac
     3781 agaatgatga tgtaaagaac tacatcgaag agaagtacag cggttccgtt atcccggcat
     3841 tctaggatac agttaccgtt ccctgtatac aatagcaaca gacagaaaca tccggaaatc
     3901 ccccacttct gaatgttacc tgatatgaga ttatgagatg gcagaggctg ctgtaaaaac
     3961 ctgaaaaaca ggaaattttg cagcagccgt ttgcccgttt tatatctgtt tcaatatctg
     4021 tttagaagga gaaatttatg atcaatattg aacacgtaag caaacaattt ggtagccttc
     4081 aggtgctgga ggatatttcc attaatatcg accagggaga tatctttggg atcatcggac
     4141 agagcggcgc cggtaaatcc acattgctgc gctgcatcaa cggactggaa aagtacaacg
     4201 gcggctctat tacagtagaa ggaaaggaga tcgcaggtct cagccagaaa gaacttcagt
     4261 tggtgcgcaa ggacatggga atgatctttc agaacttcaa tctgttaggc cgtctgaatg
     4321 tgtatgataa cattgccctt cctcttcaat tctggggaca gaataagaac agcaaggaaa
     4381 atcatgaccg tattatggaa ctgttagatc tggtcggctt aaaggataag gtaaacagct
     4441 atcccaaaca gctttccggc ggtcagaaac agagagtggc gattgcaaga gcccttgtat
     4501 taaatccaaa gatcctcctt tgtgatgagg caacctctgc attggatccg cagatcacaa
     4561 aaggtattct ggcactgctt ttaaagatca acaaagagct ggggatcacc attgttgtgg
     4621 taacccacca gatggaagtt gtaaaagaga tctgcaacaa agttgcgttc ttaaagcatg
     4681 gtaaaatgat cgctaccgga aagccggaag aactgttcgt agcgccaaat aaggacgttc
     4741 aggagttcgt aggtgacagc gtagagttcc ttccaaaaga cggcgtcaat gtccgtctgt
     4801 tctttaacga tgaaactgcc agcgacagtg ctattactca cctggcgcat gaactgggaa
     4861 tcaattttgg tatctgccag ggcaagctgg agaacttccg cggaaccatt atgggaagcc
     4921 tggtgatcaa tgtaacagaa gcagacagag agcgtgtaac tgcatggctt acagacagag
     4981 gtattttatg ggaggtgctt gaaaatgtat gatgtaatta tgaaagcctt cgggcagact
     5041 gtatatatgg tcatcggttc taccattttt tccgtgatct taggttttat cccggccatc
     5101 gtgcttacag tgacagcacc ggacggcctg cggcctaaca agatcattta cggtatcctg
     5161 gatatccttg taaatgtatt ccgcagcttc ccatttatca ttttaatggt tattgttatc
     5221 ccgtttaccc gtatgatcgc aggaaaggcc attggtacaa aggcagcttt agtgccttta
     5281 accatttctg ccattccttt tgtagccaga gtgattgagt ctgctcttcg cagtgtagac
     5341 catggtctca tcgaagcagc ccgttctttc ggcgcaggag acctgcagat catcttccgc
     5401 gtatacttaa aagaagctct tccggctatc ctttccggta tcacactgac tatgatcagc
     5461 ctgatcggtt acagcgcaat gggcggtgcc attggtgccg gcggtctggg cgatgtggcc
     5521 atccgttatg gataccaggc ctataaaatg ccgtatctga taagtaccag tattgtgctg
     5581 atcatctttg tgcaggtgat ccagagtgta ggaaacttat tatataagaa attatcataa
     5641 acgcaggctg aaccttcgtt aacagtccag gaaaaacata tatatgcata taaaaagcag
     5701 ccactctgct gcaggcccta taaatggaac cggcagagcg gctgcttttt tgtgcagtga
     5761 atgagatgat ccggtaagcc ggaccaaaat atatttaaaa gaaaaatcct ccgatcagat
     5821 aaacaatata agctgcgatc catgcaacca cacactgtaa gatcacaacg gtcagtgccc
     5881 attttccacc cagctctctc ttaatagaag caatggcagc cacacatggt gtatacagca
     5941 gacagaaaac taacaggctg gcagcggaca gagaagtcat actggatacc agtgtgctgg
     6001 tttttccaaa gaggatagac agcgtggaaa caacgctttc ctttgctaaa aatcctgtaa
     6061 taagggcagt ggcgatccgc cagtctccca gtcccattgg atgcattaat ggggcgatca
     6121 ggccggagat catagcaagg atactgtcct tagagtctgt aacaatgttt aaacgcaggc
     6181 tgaaggtctg taagaaccag atagcaaggg ttgccatgaa gataacggta aaggctctct
     6241 gaaggaaatc ctttgcctta tcccataaca gcagaccaac gtttttggca cctggcatac
     6301 ggtaatttgg cagctccata acaaaaggaa cagcttctcc gttaaataaa gttccacgta
     6361 aaataagtgc cattaaaata cccatgataa taccgccaaa atacagggct atcatcacaa
     6421 gggtgccatg ctccgggaag aatgctgcgg taaaaaaggc gtagataggc agctttgcgg
     6481 aacagctcat aaacggggtc agcaaaatgg tcattttacg gtcacgttcc gatggaagag
     6541 tacggcttgc cataacgccc ggaacggtac agccaaagcc gataagcata ggcacgatgc
     6601 tgcggccgga aagaccgatc tttcgcagca gcttatccat aacaaaggct actcttgcca
     6661 tatatccgct gtcttccagc aaagacagga agaagaacag tgtaacaatg atcggcagga
     6721 agcttaatac acttcccaca ccgttgaaaa taccgtccat aacaagggaa tggatcaccg
     6781 ggtttacatt ccaaaggatc aggaaatggt ctaccagcgt ggaaagagcg gaaatgcagc
     6841 tgtcaaggag gtcagataag aaacttccga tcacgccgaa ggtaaggtag aataccagtc
     6901 ccataatacc tgcaaaacag ggtatagcgg tatattttcc ggtaagtatc ttatccattt
     6961 tcacgctgcg cagatgttcc ttgctttctc ttggcttgat aacggaagca tcacaggttt
     7021 ttgcaataaa atcaaagcgc atatgtgcaa tggctgctgc acggtccagg ccgcgctctg
     7081 cttccatctg ggtaatgata tgctctaagg tctgtttctc gttctcatcc aggttcaagc
     7141 tgtttataat aagggaatct ccctccgcca gcttggaggc agcaaaacgc agtgggatac
     7201 cggcggcttt tgcatggtcc tcaatcaggt gcataatggc gtgaagacaa cgatggacag
     7261 caccgccgtc atcatttaca tcacaaaggt ccatggttct tggaggttcc tgatatttgg
     7321 caacgtggat ggcatgttct accagctcgt cgatgccctc atttttggca gcagaaatag
     7381 gaatgaccgg gataccaagc atttcctcca tctggttgac cataatagaa ccgccgttat
     7441 cccgcacttc atccatcata tttaatgcaa ggaccatagg gatatccagt tccattaact
     7501 gcatggtcag atacaggttt cgctcaatgt tggtggcatc tacaatgttg atgatgccct
     7561 ttggatgctc atctaaaaga aagtttcggg ttaccagctc ttcactggaa taaggggaca
     7621 tggagtagat accaggaagg tctgttacaa gggtattgct ctttccgcgg atcataccgt
     7681 ctttccggtc tacggtaacg ccagggaagt tacctacatg ctggttggaa ccggtaagct
     7741 ggttaaacag tgtggtcttg ccgcaattct ggtttcctgc aagggcaaag gtaagcagct
     7801 ctgtatccgg cagtggattt tccgttgctt taatatggta ttttccgcct tcaccaaggc
     7861 ctggatgagg cttggactgt tcaatggtgc ttaagtccgg attctggggg cggggaagaa
     7921 cgccgtccct tacatctgta atttctattt tttctgcatc tgcaaggcgc aaggtcagct
     7981 catagctgtg gatgcgcagc tccatagggt cgcccatagg ggcatatttc accatggtca
     8041 cctctccttt ggggataagg cccatgtcta aaaagtgctg gcgcaaagct ccttctccgc
     8101 caacagacaa aatcgttgca gtttttccaa tgggtagatc gcgaagtgtc atactaggct
     8161 cctttgtccc tggtatatta tagaaaagga tataccaggg gtaattttgt tgataaaaat
     8221 tatcattaat accaatctaa ggtatgacac ataagaagat ttgtcaagag aagatttatt
     8281 atataaagaa gctttgattt atagcttaag atttattttc caacagaatc aaattcatca
     8341 taaatttctt ttgaaggctc ctttgtaaga agggaaacaa tgacaatagc aaggcaggaa
     8401 aggacaaatg caggaagcag ttcataaata ttgaagtttc cgcctaatgg gcgtaccaga
     8461 tacttccata caaagaccat aacgccgcct gtaagcatac cggccagtgc gccctgcata
     8521 ttggtacggc gccagaagag ggcgaacagc accagcggtc cgaaggatgc tccaaaaccg
     8581 gcccatgcaa aggatacgat ggtgaataca gagctgtccg ggttccatgc caggaaaacg
     8641 gctacaatgg caattcccat aaccgtaaga cgggctgcat tcatggaagc ggcagtgctc
     8701 attttgatct ttaaaaagtc ctggagcagg ttctgggata caccggaagc agcagttaaa
     8761 agctgggaat cagcagttga catggtacat gccaggatac ctgcaagtac aacgcctgcg
     8821 accagtgaca gtaagaaacc attctgtcca agaagatgtg caaactggat gataacagtc
     8881 tcagactggg agctggtggt aaacattggg atagaacctg ctacagaagc gccatatccg
     8941 atgataccga taagaatggc aacgcacatg gagatcagta cccatacagt tgcgatccgg
     9001 cgggaaagta ccagtttttt ctcctcttcg atagccatga aacgaaggag gatatgaggc
     9061 ataccgaagt agccaaggcc ccatgctacg gtagatacaa tagaaagcag gccgtaaggt
     9121 gcagcagaag cagttgccct gtcatgaagc tgggttatgg agaggtaacc ggctagagaa
     9181 gtagcgttgt ttacaacttc gctccagcca ccggccatat ggatgccata aaatacgatg
     9241 acgaccaggg aaatggacat aaccacgctc tggatcaggt ctgtagtgga aactgccatg
     9301 aagccgccca taacggtata acctacgata acaactgcac caacgatcat tgcaatgtga
     9361 taatcggtac cgaaaaggct ggagaataag gttccgatgg ctttaaatcc ggaagcagta
     9421 taaggaataa agaaaacaag gatgatgatg gcggaaatgc acatcaggat atttttgtca
     9481 tctttaaaac gacgggagaa gaagtccgga atggtgatgg cattgcatac gatcgagtaa
     9541 cggcgcagac gctgtgctac gatcagccag tttaaataag taccaatgcc aaggccgatg
     9601 gcggtccagc ctgcgtctgc aataccggtg agataagcaa cgcctggcag acccatcaac
     9661 agccagctgc tcatatcaga ggcttccgca ctcatagctg ttacaaaggg accaagcttt
     9721 ctgccgccta aatagaaatc atctgcggaa tcaccgctgc ctttcttgct gaaataaaat
     9781 cctacataca ccattgcaag cagatagatc acaatggctg ccaggatacc agcctgtcct
     9841 gaaatcataa tcttattcct cctcgaaata atgatctccc tagtctttca tgcgcagaat
     9901 gatttcggga gaacataata ttgaggacga ttataacata cataaaaata gactacaata
     9961 cagaatgaag tatagaataa atactgtacg aatattaatg aaatgtgaca aaaacccagt
    10021 gtttatatag aaaaaataac tgtacgatta taataaataa aacatataca aaaaataaga
    10081 attaaaataa tatccacagc aggttgtaca tttttaagga agaaagctgc ttaaaaaacc
    10141 cggaaggacc tttgcgctgt attccgtcag ggcatattcg cctgcacaaa gacaccagtc
    10201 ataaagaagg gcccgttcgc agagagcata cagctttacc atttcattta cattggaacg
    10261 gctgcttaac tggccttcag cctgaccttt ggcgatgatc tttcgcagca gtttatagta
    10321 aatccggttg tggtctaaca gatgtttttc cccattggta gtcagctggg tagaataaag
    10381 ccttgctaac aggtccaggg aaatgctgtc ctcgatcata tgaaaaagtt ccctgtttaa
    10441 aaacagcagc tgctccatag cagtcatttc tggtgtcaga agtccggtaa gttcctcata
    10501 tttctcatca aaaagagagc ttaaagtaga aagaagggca tctttgccgt caaaatagtg
    10561 gtaaaaagag cctttggaag tgccggaaag ctcgatgatc tcttctactg tcgtatcttc
    10621 atagccctgc tcataaaata gcttccaggc tgcgcttaca atgcggcctc tggtgttgcg
    10681 ggtggatttt tttgccatgg gaggttcctc cgtttttgtt tatgtactct gtatccatta
    10741 tacataaaaa cgggtcttct gcaaagaaag acagaaaacc cgtttgaatg aaacacgatt
    10801 atatttacag aaccatagca atcacaaagt tataaatagt gcaggtgatc acgctgatgg
    10861 gaacagctac catgagaaga cggttaaaca gctcattgcg ttcttcctct ttcggggtgg
    10921 aaccaaggat cagggaaccg ccggaagaaa atggactgat agcgctggac tgggcaccaa
    10981 ggatcgtgca ggcaaataat accggcgcgc ttaaacctac agaagcagcc agattcggga
    11041 tcagcgggaa caatgccgga gcaaccactc ctgtagtgga actgaagaaa ctcatgatgg
    11101 cacctaccag gcagaaagca ataggaacca gaggctttgg tacattggaa ccgatccagg
    11161 tagaaagtgc ttcaatggtt ccggcttcta ctgcaacagc gatcagcata ccggcacctg
    11221 cgatcatgat aatggtattc cacggaactt ttgcaatggc ttctttctgt ggagccagtt
    11281 ttaataaaag ggcgatcaca gtaaagataa tggcaaccag acctacatct acttttccgc
    11341 tgatatattt aacagcagca tttcccggca tcaggatgtt taacagaggg aagatcagga
    11401 caacagccat catagccatc attaaggtaa gggtagtctt ctggtctttt gtaaagcttt
    11461 ctggtttctt aaaagtaaca cctttaccaa tatttctgtt ttctttaaag ccaaaacgga
    11521 agatagtgat caggatcagg gagaagaaga tggcaaatgc aaaaatcttc atttccatat
    11581 taaaggagtc tacagctgcg atttcaggag aagcttcata ggcagtgtca gacagtccgc
    11641 ggaaaacaac gccaagattg gaggttggga aattaccacc ggctaaagca ccgcagttaa
    11701 tggcaacagc accggtcagt ttgtccattc tggactcatc gcagatcacc agagtgatcg
    11761 gagctaaaaa tgcaagaacg gtgaaaaaag tagctcccat aactgaaagg atgactgcta
    11821 ctgcaaacag cgcataaggc agaagtcccg gaaaattgcg acaggcgtat aacagggcac
    11881 cggacatttt ctcaagggtt ccgttgatcg ctgcaaaatt ataaaataat gaaacagata
    11941 agataacaaa catggtactt gtaggccaga aaccaatgat ctgctttggc tttaatccca
    12001 taacgaaaca tccgatgatg taagcaaata caatacagaa aagaccggta ttgatcttgg
    12061 tcctgtatcc aagaaaaatt gccagtgcga ttgaaagaat aataatgata cttaacatgt
    12121 gttcccctcc tgataggtgt tatattttcc tgaagctttt attctgcaag cttcttattc
    12181 aattccaaga agcttgtaag gaatggttct tgtcatgtgg taaatatgct cctccgggat
    12241 gccgtgatct gccagatatt gcatatattc ctctaaagcc agctcccagt gcggattttc
    12301 ggtctgtccg ccgtctgtat ctaccataac gtgttcataa ccaacggcct taataagctc
    12361 cagattgtct gggagattgc tcttgtaagc accgccgccc atattctgtg cgtaacaccg
    12421 ttccaggatc acgtcataat cttttaccag gcggatctgg tcatctatgc tcatatccac
    12481 tacccaccat tccggatgag tgataacgat ctttttaacg ccggcatctt ttgcagcttc
    12541 taccactaca aatgcttctt ctggggaaac atgggctgtt ccaaggacta cgtcatagtc
    12601 acggatcagt ttaaatacat ccacaagttc cggaactacc ttcccatccc gcactaattc
    12661 gatgccttta gccggatcct ggcccatttt ctctaaatgg cgttttgcag actgggtagg
    12721 aagccagatc tcttttgcac ccagctttaa gcctttttct acggcttccg ggttgatacc
    12781 gccgatgacc ttgttcataa caacgccgcc gtacatggtg aaaccggtat tttcaccgta
    12841 tacttttttc acgtactgat tggtgagata agcacggttt actgtaaagc ccagatgagt
    12901 tttgatgaca atggctctgg cgccgactct gactgctgca tctgccagtt caaagtcatc
    12961 ataagcacgc agccttaagt ctggatttgt atgtacgtgc atatcgcaca cacctttcat
    13021 ggaaatcttt cccataataa acctccaatt tgagagaata ttttattgat ctgtttttat
    13081 cgcttatgta attataataa tctacttttg ccaacgaaaa aatacaaaat atgctataat
    13141 atatatcgga aaaaccgata gctataaaga aaacacagat tggtcagcag ggatcagcga
    13201 aacaaagggg gaaatacaga tcatggaaat gaaagaactg gattatttta ttgccatagc
    13261 agaagaaaag agtatttcaa aagcggcaga gcggctgttt atggcccagt ccagcctcag
    13321 tcagttttta agcatattgg aaaataatgt agggagtaag ctgtttatcc gtacttcccg
    13381 gggagtgcgt ctgacccagg aaggggaact gatgctccag tatgcatata agacactggc
    13441 agaataccgg tctgtacaaa acgagatcaa agatatgaaa cagttaaaaa gcggccgtgt
    13501 gatcatgggg gtcagcagtt tcagaggttc ttatcttctt ccaccagtgt taaatgcatt
    13561 ccgcctggag catccggatg tgaaagtgga gatcgtggag caaaattcca tggtattaga
    13621 gcagcagctg ttgtccggcg agatcgatat tgccctttta gttatgtctg aggcagatcc
    13681 cagactgaat atagagtggg tcttgaaaga tgagatatgc cttattacca gccataccta
    13741 cccggtcatg gagtttgcaa gaaataatga ggataatccg gactccagga taccacagta
    13801 tgtgaattta aaagatacac tttcctatga atatcttctc agcgattatg atacgatcct
    13861 gggccgggaa gcaaggcaga tatttaataa gaatggaata caaccgattg ccagcaatca
    13921 caagctgtca gcccttatgt cagcagctat gggagcagca ggactgggac ttgcatttac
    13981 ctattacagt tccagacatt atttcagaaa tgcagagttt ttatctctgg gtgcagaaag
    14041 tgcttttatt gatctgggaa tagcactgcc gcctggcaga taccattcca aagcggctct
    14101 tgcatttaaa gatacgctga aagaggtcct gcaggcggca gatggctgaa ccctatctta
    14161 ttacaaaaat aaacgttact tctcttcccg actgcagatg gcaagtcgat gctggtattg
    14221 atatttctcc tttgtagcca tcccaccccg gatatgacgc tcggatttgt tcagatccag
    14281 gactactctt gtctgggcgg cgagagccgg attgatggag atcagtctct ccgtgcagtc
    14341 cttgtgtact gtgctcttac tgatcccaaa gcgttttgct gtctgccgga cagtagcgtt
    14401 gtgatctatg atgtaattgg caatggcgac agctcgttct tcaatataat ctttcatggg
    14461 ttatcccctt gagaacgttt tttacatcat attaggacaa tgggaaattt atgcaggttc
    14521 gtgtacaatc atctaaaagt gtccgccaga aaaaaacatt tgtattttac agacatattg
    14581 tgtataatgt tttccgttgc gtatttttaa cactttacca aatagaaggg aataggaaaa
    14641 gagtatgaag aagatagttc aggcctggac cggcatcagc ctggtgcaga ggattgtggc
    14701 tggtcttgtg atcggtgctt tattaggaat gtttgttccg tccgaaggag ggatcgccat
    14761 tttaggtgat gtatttgttg gcgcgttaaa agccattgcg ccattgttag tatttttctt
    14821 agttatcagt tctctgtgct acgcaggaaa aagccacgga ggcgtcatcc gtacagttat
    14881 tattatgtat atgttcagta ccttccttgc agctatcgta gcagttactg caagcagtgt
    14941 atttcctgta acactgacac tggtggatgc ggcaactgat atggcagcac ctcagggtgt
    15001 agcagaagta ttaagaacac ttcttatgaa tgtggtaaca aacccggtac agtctttagt
    15061 atctgcaaac tacatcggca tccttgtatg ggccgttctt atcggtatcg cacttcgtaa
    15121 ggcatctgat accaccaagc agtct
//
LOCUS       DBMDUA010000052        15093 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2174_length_15093_cov_4.047826, whole genome shotgun sequence.
ACCESSION   DBMDUA010000052 DBMDUA010000000
VERSION     DBMDUA010000052.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 15093)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15093)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15093
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2174_length_15093_cov_4.047826"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            142..1413
                     /locus_tag="ACZPCL_05810"
                     /pseudo
     CDS             142..1413
                     /locus_tag="ACZPCL_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012544.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
     gene            1479..2900
                     /locus_tag="ACZPCL_05815"
     CDS             1479..2900
                     /locus_tag="ACZPCL_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR1162026.1"
                     /translation="MSDTKNTENESGGLKKSLRLIYVYAIAAGAIFTFIGYWDTIFYE
                     YCGAGTFLGFILMTLLILPIAMVYCELAPLFPRAGGELIYNTVGINKHIGFFSSWLIM
                     AAWIAVPPSAVMAIVQWMFHVLHIQSSFLLIEGVALVALIGYCALSLQNVEIAGKIQL
                     YMLMFAIAGCIVATVAFLFSGVWSFDNFKNFFYSQVGSHFGIPSWIIGMALLITPFFG
                     FETVPQMVEEGDFPIKDSNKAILGSIVSCGIVYSLFFFGLGGMPVQPLVEEGGAAVNG
                     FLAITMMEQLGGGWGVFAVIFGIAAILCAIGTCLLGFWLSTVRLMYAMGENNFLPKAF
                     TKLNSHQQPVLPNILLLIISLVFLLLQNATTFMNDFFNLMSFGCACAYALTMISAMRI
                     HKKYPNWVSPYKLKGGNFTRVLALIIAVVIAFFCTLGQSAGSWKSFGIYLGVGVLLWL
                     WMVLVNWKKHPVEIETVEGLKKF"
     gene            3000..3797
                     /gene="ucpA"
                     /locus_tag="ACZPCL_05820"
     CDS             3000..3797
                     /gene="ucpA"
                     /locus_tag="ACZPCL_05820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009429448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HIR1162027.1"
                     /translation="MAKLDGKVALITGAAAGLGEGISTAYAKYGAKLIMVDLSPEVEK
                     TAEKLRKEYNAEIITVVANVADRAQMDAAAAQGVEKFGEINVACCNAGVCRLAPFEEM
                     DDKTRDFHIDVNIKGVWNTCKAVIPYMLKQGGGNIVIASSVTGDIVADAGEAAYAMTK
                     AALVGLTKCLAVEYASRNIRVNCSQLGYARTPMVEKMAVESNPENPEAAIADIAVGVP
                     MKRLAKPIEVGELFAFLGSDESSYLTGSQIVIDGGSTLPETMSIGTN"
     regulatory      3840..3948
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4212..5018
                     /gene="thiD"
                     /locus_tag="ACZPCL_05825"
     CDS             4212..5018
                     /gene="thiD"
                     /locus_tag="ACZPCL_05825"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR1162028.1"
                     /translation="MKTALTIAGSDSSGGAGIQADIKTMMANGVFAMSAITALTAQNT
                     TGVEAILNATPEFLGQELDCIFTDIYPDAVKIGMVSDKELICMIAAKLKQYDAKNVVV
                     DPVMVATSGARLISEDAIETLKAELFPLAKILTPNIPETEELTGMKITSAADMEAAAK
                     KISETYNCAVLCKGGHKLNDANDLLWSEGKGRWFAGKRIDNPNTHGTGCTLSSAIASN
                     LAKGFDLETSVERAKDYISGALAAMLDLGKGSGPMDHGFAIDNEYTREAK"
     gene            5021..6181
                     /gene="cytX"
                     /locus_tag="ACZPCL_05830"
     CDS             5021..6181
                     /gene="cytX"
                     /locus_tag="ACZPCL_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HIR1162029.1"
                     /translation="MEKKTSLFDNGLIWFGAGVSLAEILTGTYFSTLGFGRGLAAILL
                     GHVIGCVLLFLAGVIGGNSRLSAMETVKSSFGNKGGLFFALLNVLQLVGWTAIMIYDG
                     ALAAGGIFSSGHWIWCLVIGVLIIVWIMVGITNLGKINTVAMTALFLLTLVLCKVIFF
                     SGNSILTEPDDSLSFGAAVELAVAMPLSWLPLISDYTREAEKPVAATAVSAVVYGLVS
                     CWMYLIGMGASILTGESDIAQIMLKAGLSVIALIIVVFSTVTTTFLDAYSAGISNESI
                     FPQFKGQYVAVAVTVVGTIGAIVFPMDDITGFLYLIGSVFAPMIAIQIADYFILHRDR
                     RKTAFDTRNLVIWVIGFVLYRILMNVDLPVGNTLPDMIITIILCVIAGAVLH"
     gene            complement(6287..8176)
                     /locus_tag="ACZPCL_05835"
     CDS             complement(6287..8176)
                     /locus_tag="ACZPCL_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1162030.1"
                     /translation="MKILSLHIDGFGKFHDRDISFEDGLNVVYGKNEAGKSTLHTFIK
                     GMLFGIERQRGRASKNDTYSKFQPWTQSGVYEGFLRVECDGEVYRIERRFQKGDKRLC
                     VINETSGKEEENTTALLDKLRCGLSETAYDNTISIGQLKCATDGGMVSELRNYIANLN
                     TSGSIALNITKASAYLKNQRKQLEAHFVPEAAKSYANILGEIRKIEQEISAPEYANHL
                     TARRQEKGQLKNDLDKKQKEKEVLLEKAAKGRQILVNSQFENQASVEKYTEEARKHYQ
                     DYNNALEACSKHSRTIGAVFLFLMAVPGLASGLFLLLFPNTAISLLSGFAPRFLQLSG
                     SLIKIPALLAILCGILFAVAGSMLLARSRHFKKQLDTETRQLQEIFSKHLQDPSISEE
                     AMEALEGKLAGFLRLSKAVEQSENTLNTMAADISRLQEKEECVSEEIEKQQRLQWELE
                     QKLEQLSAKKNEAEQLKHVLTENERIQEELDALDLAQETMTHLSTSIRDSFGLYLNKT
                     ASELIEGITGGIYRSMSIDQNLNIFMNTPTRLVPIDQVSSGTMDQIYLAVRLAAAKLV
                     ISDRGTMPLIFDDSFVLYDDDRLKTALKWLAGAWDSQCIIFTCHQREEAILKSEGIEH
                     HLIQI"
     gene            complement(8181..9251)
                     /locus_tag="ACZPCL_05840"
     CDS             complement(8181..9251)
                     /locus_tag="ACZPCL_05840"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1162031.1"
                     /translation="MKFIHTGDIHWGMTPDADKPWGSERAQAIKDTFKKIIAQAGKMQ
                     ADCLFISGDLFHRQPLVRDLKEVNYLFTTIPNVKVILIAGNHDRIRENSALLSFSWSP
                     NVTFFMDPDLNSVYFEDINTEIYGFSYHTREIKKPLLEDMQVSVNNHIQILMAHGGDT
                     AHLPINFNSLELSPFSYIALGHIHEPQVISEDKIAYCGSPEPLDLTETGVHGYFTGEI
                     HPETHKLTHLEFVPAATLQYLPLAVKVSRETTNMELADRIMAEIRKRGEENIYRFRLK
                     GMRDPDIEFDLSSLEAQFRIAEIIDDTEPDYDFSALFAEHSSDMIGFFIQALQKEDMS
                     PVEKKALYYGVNALLHTTDERS"
     gene            complement(9366..10757)
                     /gene="asnS"
                     /locus_tag="ACZPCL_05845"
     CDS             complement(9366..10757)
                     /gene="asnS"
                     /locus_tag="ACZPCL_05845"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIR1162032.1"
                     /translation="MDLVTVRELYKNPEKFLDKEITVGGWIRSLRDSKAFGFIVISDG
                     TYFETLQAVYHDTLENFAQVSKLGVGTAIIVKGTLVATPQAKQPFEIQATEVTVEGPS
                     ASDYPLQKKRHSFEYLRTISHLRPRTNTFQAVFRVRSLIAYAIHQYFQEKDFVYVHTP
                     LITGSDCEGAGEMFQVTTMDLNNIPKNEDGTVDYKQDFFGKPTNLTVSGQLNAETYAM
                     AFRNVYTFGPTFRAENSNTTRHAAEFWMIEPEMAFADLDDDMRVAEGMLKYVIKYVLE
                     HAPEEMNFFNNFVDKGLLDRLNNVLNSEFGRITYTEAVELLMKHNDKFDYKVSWGCDL
                     QTEHERYLTEEIFKKPVFVTDYPKEIKAFYMKMNPDGKTVAAVDCLVPGIGEIIGGSQ
                     REDDYDKLVARMDELGLKKEDYGFYLDLRKYGSTRHAGFGLGFERCVMYLTGMSNIRD
                     VIPFPRTVNNCEL"
     gene            11154..12428
                     /locus_tag="ACZPCL_05850"
     CDS             11154..12428
                     /locus_tag="ACZPCL_05850"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR1162033.1"
                     /translation="MIKKEMIAMLLAGGQGSRLGVLTQKVAKPAVSFGGKYRIIDFPL
                     SNCINSGVDTVGVLTQYQPLRLNSHIGIGIPWDLDRNVGGVTILPPYERSKGSDWYTG
                     TANAIYQNLEYMEAYNPEYVLILSGDHIYKMDYEVMLDYHKANNADITIAAMPVPIEE
                     ASRFGILITDEHNRITEFEEKPAVPRSNLASMGIYIFSWPVLKEALIKMKDEPGCDFG
                     KHIIPYCHAKGDRSFAYEYNGYWKDVGTLGSYWEANMELIDIIPEFNLYEEYWKIYTK
                     SDVIPPQYIASDAHIERSIVGEGTEVYGDVINSVIGAGVTIAKGAVVKDSIVMQGSVI
                     GAGTSVEKAIIAENVKVGSDVQIGVGEYAPSTYDQKVYQFDLATIGENSIIPDGVKIG
                     KNTAIAGETTVGDYPDGLLASGNYIIKAGGVK"
     gene            12425..13546
                     /gene="glgD"
                     /locus_tag="ACZPCL_05855"
     CDS             12425..13546
                     /gene="glgD"
                     /locus_tag="ACZPCL_05855"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HIR1162034.1"
                     /translation="MRAIGIVLAGGNSKRMRELSSKRAVAAMPVAGSYRSIDFALSNM
                     TNSHIQNVAVFTQYNSRSLNLHLSSSKWWDFGRKQGGLYVFTPSITPENGDWYRGTAD
                     ALYQNLTFLKNSHEPYVVITSGDGVYKLDYNKVLEYHIEKKADITVVCKDMEEGTDVT
                     RFGCVKLNDDGRITDFEEKPMASDATTISCGIYVIRRRQLIELLERCAAEDRYDLVND
                     ILVRYKNLKRIYAYKLDSYWSNIATVDSYYKTNMDFLKPEVRDYFFKQYPDVYTKIDD
                     LPPAKYNPGAVVRNSLVSSGCILNGTVENSILFKKAYVGNNCIIKNSIILNDVYIGDN
                     TVIENCIVESRDTIRANTTYIGTPDNIKIVIEKNERYTL"
     gene            13641..13922
                     /gene="spoVG"
                     /locus_tag="ACZPCL_05860"
     CDS             13641..13922
                     /gene="spoVG"
                     /locus_tag="ACZPCL_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HIR1162035.1"
                     /translation="MQVTDVRVRRVEKEGKMKAIVSITLDNEFVIHDIKVIEGEKGLF
                     IAMPSRKAADGEYRDIAHPINSGTREMIQSIILDKYESTLLENEEEALA"
     gene            14376..>15093
                     /locus_tag="ACZPCL_05865"
     CDS             14376..>15093
                     /locus_tag="ACZPCL_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012711.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR1162036.1"
                     /translation="MISYENTKTVRDLVWNTGREYAGRVFLRYEENDVVYEVTYDEFA
                     TQCRGVAAWLREQDAAAGHKVRVGLMGSSSHHYLVFLLGVMASGNVVVPLDVQLNEET
                     FADNVNRSDLDILFYDWDHYGLVEAVKEKCPRLRACISLQHGKHVPCSDQILKGYAGI
                     SVDPVVSEEDCAMVLFTSGTTGRGKGVMLSNRNLMDNNFCTTDKDHPEKEVYLNILPI
                     HHVFCINGDVFTVMRYGSVLC"
BASE COUNT         4071 a         3139 c         3653 g         4230 t
ORIGIN      
        1 cagatatggg cagaatccgg aaaatcggct ctgcccattg taactattca caaatcttat
       61 atcagttttt aacgtttaca caaaacttga aacggtctcg taaactattg ggattctgtc
      121 ttttacggat actgcggaag cggtacattc cttgcatttg cactgatgac agtggctatt
      181 cttccggtag cccttgtata cagtgaactg gcatctctgt tccatacatc aggcggagag
      241 ctgatctata acacagttgg tatcaacaaa cacgtaggtt tccttgcttc ctggctgatc
      301 atggcagcgt ggatctctgt tccgccggct gttgttatgg cgatcatgac atgggtaaat
      361 aagacctttg ccctgggact tggaacatgg cagatggtag gctgtgcagc ggttctttta
      421 gtactgtatt tctttatgag tatccagaat gtacagttct tagttaaggc tcaggcaggt
      481 atgttgttct gcaacattac cgttacaatt ttaacaggat ttatccttct gttctccggt
      541 cactggcata tttcaaattt tggaaatatt ttccagtcta ctctggactc cgtatatggt
      601 attcctggat gggtcattgg catggcactt ctgatcacac catacttcgg atttgaaaca
      661 gtacctcaga tggttgagga aggtgacttc ccgatcaaga gttccaagaa agcaatctgc
      721 ggttccattt taacctgtgg ctgcatttat gtgttctatt atttctgcgt agcaggtctg
      781 gatggattaa aggatgtgta tacaaacttt gcacaggatg gtttcttaac cattgctacc
      841 atgcagaatg tactgggctg gacattctgg ccgatcattg taggtatcct ttcctgcctc
      901 ttaggtatgg gcgcttcctt actgggtttc tggatgtcta ccgtccgtat gctgtactct
      961 atggcaaata agaacttcct gccaaaggta ttcgcagctg taaacaaaaa tcagcagcca
     1021 atgcttccaa acgtattcct gctggtatta tctcttatct ttatcgtact tcagaactcc
     1081 tcagacttta tgagcgcgtt cttcaacctg atgagctttg gctgtgcatg tgcatatgcg
     1141 ctgactatga tctccgcagt atgtattcat agaaaacatc cagactggaa gagttccaac
     1201 accgtaaagg gcggtgatgc gttccgtctc tttgcaatgt gtatcgcagt tgctatcgca
     1261 ttcttctgta cactgggaca gggaattgat tcctggatct gctttggcgt atatctgggt
     1321 gtaggtgttg tgatctggct ttggatggta cttgtaaact ggaagaaaca tccggtagag
     1381 atcgagactc cggatggtat ccagaagttc taaaagaaag ttcagaaaag tttaaatgac
     1441 atggcagaca tgcctggata aagaaggagc ataaaaacat gtctgataca aaaaatacag
     1501 aaaatgagtc tggcggactg aagaaaagtc ttcgtctgat ctatgtatat gccattgccg
     1561 caggtgctat tttcaccttt atcggatatt gggataccat tttctatgaa tattgtggtg
     1621 cgggtacatt tttaggattt atcctgatga cgctgttgat ccttccgatt gctatggtct
     1681 actgcgaact ggcaccgttg tttccaaggg caggaggaga gctgatctac aatacagtag
     1741 gtattaataa acatattggt ttcttctctt cctggctgat catggcagcc tggatcgcag
     1801 ttccgccgtc tgcagttatg gccattgtcc agtggatgtt ccatgtactt catatccaaa
     1861 gcagcttcct tcttatagaa ggagttgctc tggtcgctct tattggttat tgtgctctga
     1921 gcctgcagaa tgtggagatc gccggtaaga tccagttgta catgctcatg tttgccattg
     1981 caggttgtat cgtggcaacg gtagccttcc tgttctcagg tgtgtggagc tttgataact
     2041 ttaagaactt tttctattca caggtgggca gccattttgg cattccgtcc tggatcatag
     2101 gaatggctct tttgatcact ccgtttttcg gatttgaaac agtgccgcag atggtagaag
     2161 agggagactt cccaattaaa gactctaaca aagctatcct cggttccatc gtttcctgcg
     2221 gtattgtata ttccctgttt ttcttcggac tgggcggcat gccggtacag cctttggtag
     2281 aagaaggggg cgcagctgtt aatggttttc tggccatcac catgatggag cagttaggcg
     2341 gcggctgggg tgtatttgca gtgatctttg gtattgcagc gatcctttgt gccattggca
     2401 cctgcttact tggtttctgg ctgtcaaccg ttcgtctgat gtatgcaatg ggagaaaaca
     2461 atttccttcc aaaggcattt acaaaattaa acagtcatca gcagccggta ttaccaaata
     2521 tcctgcttct catcatatcc ctggtattcc tgcttttgca gaatgccacc acctttatga
     2581 atgacttttt caatctgatg agctttggct gtgcctgcgc atatgcgctg acgatgatct
     2641 cagcaatgcg gatccacaag aaatatccaa actgggtatc tccttataaa ttaaaaggtg
     2701 gaaatttcac aagagtatta gctcttatca ttgcagtagt catcgctttc ttctgtacgt
     2761 taggacaaag cgctggttcc tggaagtcat ttggaattta cctgggagta ggagtcctct
     2821 tatggctgtg gatggtcctt gtaaactgga aaaaacatcc ggtagagatc gaaacagtgg
     2881 aaggcttaaa gaagttttag aaacttaatt ttgaacctgt acgggaaacc gtctggcata
     2941 tataataact ataatagtta aaacaataac aattttttat atgtaaagga gaatgtaaaa
     3001 tggctaaatt ggatggtaag gttgcattaa ttacaggagc tgctgcagga cttggtgaag
     3061 gtatttctac cgcatacgca aaatacggcg caaagctgat catggtagac ttatctccgg
     3121 aagtagaaaa gactgctgaa aagctgagaa aagaatacaa cgcagagatc attactgttg
     3181 ttgcaaacgt agctgaccgc gcacagatgg atgcagctgc tgcacagggt gttgaaaaat
     3241 ttggtgagat caatgttgca tgctgcaacg caggtgtgtg ccgtctggct ccttttgagg
     3301 aaatggatga taagacccgt gatttccaca ttgatgtaaa tatcaagggt gtatggaaca
     3361 cctgtaaggc agttatccca tacatgttaa agcagggcgg cggcaacatc gttatcgctt
     3421 cttctgtaac cggtgatatc gttgctgatg ctggtgaagc agcatacgct atgacaaagg
     3481 ctgcattagt aggtctgacc aagtgcctgg cagttgagta tgcaagccgc aatatccgtg
     3541 taaactgctc acagttagga tatgcacgta caccaatggt tgagaagatg gcagttgagt
     3601 ccaatcctga gaacccagag gcagctattg cagatatcgc tgtaggtgtt cctatgaagc
     3661 gtcttgcaaa accgatcgaa gttggcgaac tgttcgcatt ccttggaagc gacgaatcca
     3721 gctacctgac cggatcccag atcgttatcg acggtggcag cacactgcca gagaccatgt
     3781 ctattggtac caactaaaaa taacttgaaa atcaaataaa accgcactat aatagtaaag
     3841 aactggaaaa ggggagctgg cagtcagtcg gctgagagtg ggatgtgttt cccagacccg
     3901 taacctgatt tggataatgc caacgtaggg atataaaacc gaggtctttt tagagtgtga
     3961 tcttagcgga tatgtctgtt gcaggcatat ccgctttttc gtgtgcaatg agccaaagtc
     4021 cgcacttgcg ggagaccatg gcgaatgccg cgaaaaccgg aaaaatgcgg agcttttttc
     4081 cggtcttagg tcttccaggc ggcatattgg gggcaccacc ttatgtcgca taataaaacg
     4141 tggcctgcag tagaacatgt caaacgtaca ttgtatacac tgtgggaatg ctgagaaggg
     4201 agaagacgat tatgaaaaca gcattaacga tcgccgggag tgattccagc ggaggcgccg
     4261 gtattcaggc cgacattaag acaatgatgg caaacggagt atttgccatg agtgcgatca
     4321 cagcactgac tgcgcagaac acaacaggag tagaggcaat tttaaatgcc actccggaat
     4381 ttttaggaca ggaattagac tgtattttta ctgacattta tccggatgca gtaaagatcg
     4441 gtatggtatc agacaaagag ctgatctgca tgatcgcggc aaagttaaag cagtatgatg
     4501 cgaaaaatgt agtagtagat ccggtcatgg tagcgaccag tggagcgaga ctgatcagtg
     4561 aagatgctat tgagacttta aaggcagaat tattcccgct ggcaaagatc cttacaccaa
     4621 atataccgga aacagaagaa ctgacgggaa tgaagatcac atctgccgca gatatggaag
     4681 ctgccgcaaa gaagatctct gagacatata actgcgccgt actgtgcaaa ggcggacata
     4741 agttaaatga cgccaatgac ctgctttgga gcgaaggaaa aggacgctgg tttgcaggaa
     4801 agcgtattga taatccaaat acccatggaa caggctgtac tttatcatct gccattgctt
     4861 ccaacctggc aaaaggcttt gatctggaaa cttccgtaga gcgtgcaaag gactatattt
     4921 caggcgcttt ggcagcaatg ctggatcttg gaaaaggcag cggccctatg gatcatggtt
     4981 ttgccattga taatgaatac acgagggagg caaagtaaga atggaaaaga aaacttccct
     5041 ttttgataat ggactgatct ggtttggcgc aggtgtttcc ctggctgaga tcctgacagg
     5101 tacttatttt tccaccctgg gatttggccg tggacttgcg gctatcttac tgggacatgt
     5161 gatcggctgc gttcttctgt ttttggcagg cgtcatcggc ggaaacagcc gtttaagtgc
     5221 tatggagaca gtaaagagca gtttcggtaa taagggcgga ctgttttttg ccctgttaaa
     5281 tgtattacag ctggttggct ggaccgcgat catgatctat gacggcgcac tggcagcagg
     5341 cggtatcttt tcttccggac actggatctg gtgcctggtt atcggcgttc tgatcattgt
     5401 atggatcatg gtaggtatca ccaatcttgg aaagatcaat accgttgcaa tgacagcttt
     5461 gtttttactg acactggtac tgtgcaaggt gatctttttc agtggaaaca gtattcttac
     5521 agagccggat gacagccttt cctttggtgc tgctgtagaa ctggcagtag ctatgccatt
     5581 atcatggctg ccgctgatca gtgactatac cagagaggca gaaaagccgg tagccgcaac
     5641 tgcagtcagc gctgtggtat atggtctggt aagctgctgg atgtatctga tcggtatggg
     5701 cgcatctatc ctcactggtg agtctgacat cgcccagatc atgttaaagg ccggtttaag
     5761 tgtgatcgcc ctgatcatcg tagtattttc aactgtaaca actaccttcc tggatgctta
     5821 ttctgcaggt atctccaacg agtccatttt cccacagttt aagggacagt atgtggcagt
     5881 agctgttacg gttgtgggaa ccattggcgc tattgtgttc ccaatggatg atattaccgg
     5941 atttttatat ctcatcggtt ctgtatttgc gccaatgatc gccattcaga tcgcagatta
     6001 ttttatttta cacagagaca gaagaaaaac tgcatttgat accagaaacc tggtgatctg
     6061 ggtcatcggt tttgttctgt accgtatcct gatgaatgtg gaccttcctg taggaaatac
     6121 actgccggat atgatcatta ccattatttt gtgtgtgatc gcaggtgcgg tattacattg
     6181 aggtaacaaa cataagaaaa tccccgtgtc tgtacatatt tgtacataga cacggggatt
     6241 gttgttatct acctggaaaa actatcaggt gacatgtctg tttagtctaa atctgtatca
     6301 ggtggtgctc aattccttcg ctctttaata tagcttcttc cctctgatga caggtaaaga
     6361 tgatacactg gctgtcccag gcacctgcaa gccattttaa ggcagttttt aaacggtcat
     6421 cgtcatagag gacgaaactg tcatcaaaga tcagtggcat ggtgccgcgg tcgctgataa
     6481 ccagctttgc tgcggcaaga cgaactgcaa ggtagatctg gtccatagtt ccgctgctga
     6541 cctggtcaat aggcaccaga cgggttggtg tgttcataaa gatatttaag ttctggtcaa
     6601 tgctcatact gcggtagatg ccgccggtaa taccttcgat cagttcagat gcagttttat
     6661 ttaagtaaag accaaaagag tcgcggatgg aagtagataa atgggtcatg gtttcctggg
     6721 ccagatcaag agcatctaat tcttcctgaa tgcgctcatt ttctgtcaga acatgtttta
     6781 actgctctgc ctcattcttt ttggcagaca gctgttctaa tttctgttcc agttcccact
     6841 gaagccgctg ctgtttctcg atctcttctg atacgcattc ttctttttcc tggagcctgc
     6901 tgatatcagc tgccatggta tttaaggtat tttcagactg ttccacagcc ttgcttaaac
     6961 gcaggaaacc ggccagtttt ccctctaacg cttccattgc ctcttcagaa atagaagggt
     7021 cctgcagatg ctttgaaaag atctcctgaa gctgtctggt ttctgtatcc agctgctttt
     7081 taaaatggcg gctgcgggca agtagcatac tgccggctac agcaaacagg ataccgcaga
     7141 gaatggctaa taaagcaggg atctttataa gagatccaga aagctgtaaa aaccggggcg
     7201 caaatcctga aagcagggaa atggcagtat ttgggaataa gaggaggaac agaccgcttg
     7261 ccagcccggg gactgccatc agaaacagaa aaacagctcc gatcgtgcgg gaatgtttgc
     7321 tgcaggcttc taaggcgttg ttgtaatcct ggtagtgttt ccgggcttct tctgtatatt
     7381 tttctacaga ggcctgattt tcaaactggc tgttgaccag gatctgccgg cctttagccg
     7441 ctttttccaa aagcacttct ttttcttttt gctttttatc cagatcattc tttaactgtc
     7501 ccttttcctg gcgcctggct gtaaggtggt tggcatattc aggggcagag atctcctgct
     7561 cgatcttgcg gatctcacca agaatattgg cataactttt agctgcttcc ggaacaaaat
     7621 gggcttccag ctgcttgcgc tggtttttta aataagcgct ggctttggtg atatttaaag
     7681 caatgcttcc ggaagtgttc agatttgcga tataatttcg cagttccgag accattccgc
     7741 cgtcagtggc acattttaac tgaccgatac tgatggtatt gtcataggca gtttcagaca
     7801 ggccgcagcg cagcttgtcc aataatgcag ttgtattttc ttcttctttt cctgaggttt
     7861 cgttgatcac gcacaggcgc ttgtccccct tctggaaacg gcgctcaatg cggtaaactt
     7921 ccccgtcaca ttcaacccgc aggaaacctt catagacccc gctctgggtc cagggctgaa
     7981 atttggaata ggtatcattt ttagaagcgc ggccccgctg gcgctcaatg ccaaagagca
     8041 tgcccttgat aaatgtatgt aaagtggatt ttccggcttc attttttcca tagactacat
     8101 ttaagccatc ttcaaaggaa atgtccctgt catgaaattt tccaaagccg tcaatatgca
     8161 gactgagtat cttcattggg tcagctcctt tcgtcagtag tgtggagtaa ggcatttact
     8221 ccataataca gtgctttttt ctctacagga ctcatatctt ctttctgaag tgcctggatg
     8281 aaaaatccga tcatgtcact ggaatgctct gcaaacaggg cagaaaaatc gtaatccggc
     8341 tctgtatcgt caataatttc tgcaatgcga aactgtgctt ccaaagaaga taagtcgaat
     8401 tctatatctg ggtcgcgcat gccttttaaa cggaaacgat aaatattttc ttccccgcgt
     8461 ttgcggatct ctgccatgat ccggtcagcc agttccatgt ttgtggtttc tctggaaact
     8521 tttactgcca gaggcagata ttgaagggtg gcagcaggaa caaattccag gtgtgttaat
     8581 ttatgggtct caggatggat ctcgccggta aaatatccat ggactcccgt ttctgtcaga
     8641 tccaaaggtt ccggtgatcc acagtaagca atcttatcct cagagatcac ctgaggttcg
     8701 tggatatgtc ccagggcaat gtaagaaaat ggggataact ccaggctgtt aaagttaata
     8761 ggcagatggg cagtgtctcc gccatgtgcc atcaggatct ggatatgatt gttcacggaa
     8821 acctgcatat cttctaacag aggttttttg atctctctgg tgtgataact gaaaccgtaa
     8881 atttccgtat tgatatcctc aaaataaacg gaattaaggt ccgggtccat gaaaaaagta
     8941 acattggggc tccatgaaaa gcttaaaaga gcggagtttt cccgtatgcg gtcatgattt
     9001 cctgcaatga ggataacttt tacattggga atggtggtaa acagatagtt gacttctttt
     9061 aagtcccgca ccagtggctg gcgatggaac agatcaccgc tgatgaacag acagtctgcc
     9121 tgcatttttc cagcctgggc aatgatcttc ttaaaggtat ctttgatcgc ctgggcccgt
     9181 tcacttcccc agggtttatc tgcatcggga gtcattcccc agtggatatc acctgtgtgg
     9241 ataaatttca tgaatttctc ctttaaatag atatactccc gaaacgtttc ttgcacctgc
     9301 gcaaaagagt ctcgagagta catatggtgc aaaacagatg gattgctttg cgtcgtatat
     9361 tgatattata actcgcagtt atttacagtt cttgggaatg ggataacgtc gcggatgttg
     9421 gacataccgg tcaggtacat tacgcagcgc tcaaagccaa gtccgaaacc tgcatgacgg
     9481 gtggaaccgt atttacgaag atccaggtaa aagccgtaat cttctttctt aagtcccagc
     9541 tcatccattc ttgcaaccag cttgtcatag tcatcttcac gctggcttcc gccgataatc
     9601 tcgccaattc caggaaccag acagtctaca gcagcaacgg tcttgccgtc tgggttcatc
     9661 ttcatgtaga atgccttgat ctcctttgga taatcagtaa cgaatactgg tttcttgaag
     9721 atctcttcgg tcaggtaacg ctcatgctca gtctgcaggt cgcagcccca ggataccttg
     9781 tagtcgaact tgtcattgtg cttcattaac agttccactg cctcggtgta ggtaatgcgt
     9841 ccgaactcag agttgagaac gttgtttaaa cggtctaaca gacccttgtc tacgaagttg
     9901 ttgaagaagt tcatctcttc cggagcatgt tctaatacat acttaatgac atactttaac
     9961 atgccttctg caacgcgcat atcatcatcc agatcagcga aggccatctc cggctcgatc
    10021 atccagaact cagctgcatg acgggtggtg ttggagttct ctgcacggaa ggtaggtccg
    10081 aaagtatata cgttgcggaa tgccattgcg taggtctctg cgtttaactg gccgcttaca
    10141 gtcaggttgg ttggctttcc gaagaaatcc tgcttgtaat caacggttcc gtcttcattc
    10201 tttggaatgt tgttcagatc catagtagta acctggaaca tttcacctgc accttcacag
    10261 tcgcttcctg tgatcagtgg ggtgtggaca taaacgaagt ccttttcctg gaaatactga
    10321 tggatggcat aggcaatcag ggaacgtacg cggaatacgg cctggaaagt gttggtcctt
    10381 ggacgtaaat gtgaaatagt gcgcaggtat tcgaaagagt ggcgcttttt ctgcagtggg
    10441 taatcagatg cagatggacc ttcaacagta acctcagtag cctggatctc aaatggctgc
    10501 tttgcctgtg gggtagcaac taaagtacct ttaacgatga tagcggttcc aacgcctaac
    10561 ttggaaacct gagcgaagtt ttccagagtg tcgtggtaaa ctgcctgaag tgtctcgaaa
    10621 taagttccgt cgctgataac gatgaaaccg aatgccttgg aatcacgaag gctgcggatc
    10681 catccgccaa cggtaatttc cttgtccagg aacttttccg gatttttata aagttctctt
    10741 actgtaacta aatccatgat gagtaatatc tccttttctc caatacaaaa tttctaaaag
    10801 caaaaatgca atattataag gtattataag gtatttcaca taatgtttca aggttttcat
    10861 gcgtttatgc aggttttcct agcgctttta aatgcagggt cccggtacag ccatgaaaag
    10921 gaaaaaatgt cccacctacc aacacacttg gttaatggcg ggggacatat agtgcccgga
    10981 ggatttttag ggcacaacat cttgggaaaa gtgtaaaata taaaacatga tatcgaaaaa
    11041 tgtcggaaaa ctgcataaaa aacacaaaat atattaaaat atttgtgtac tatttataaa
    11101 gtttgtggta taattatgtt taccaaaatt atataacagc aagaggtgat aacgtgatta
    11161 aaaaagaaat gattgccatg cttttagctg gcggacaagg aagccgtctt ggcgttctta
    11221 cacagaaagt agccaagccg gcggtatctt ttggggggaa gtaccgaatc attgattttc
    11281 cgctgagtaa ctgtatcaac tccggtgttg acaccgttgg tgttctgaca cagtaccagc
    11341 ctttacgttt aaattcacat atcggaattg gtattccgtg ggatttggac agaaatgttg
    11401 gcggcgtgac aatcttacct ccatatgaaa gaagcaaggg aagtgactgg tataccggta
    11461 ctgccaatgc tatttatcag aacctggagt atatggaagc atataaccca gagtatgtgc
    11521 tcatcctttc cggtgaccac atttacaaaa tggactatga agtaatgctt gattatcaca
    11581 aagcaaataa tgcggatatc accattgctg ctatgccggt accgatcgaa gaagcaagcc
    11641 gtttcggtat cctgatcact gatgaacata accgtattac agagtttgag gaaaaaccag
    11701 ctgttccaag aagcaacctg gcttctatgg gtatctatat cttcagctgg ccggttctga
    11761 aagaggctct gatcaagatg aaggacgagc ctggatgtga ctttggtaaa catatcattc
    11821 catactgcca cgcaaagggc gaccgcagct tcgcatatga gtacaatggc tactggaagg
    11881 atgtaggtac tcttggctct tactgggaag caaacatgga gctgatcgat atcattcctg
    11941 agttcaacct gtatgaagaa tactggaaga tctacaccaa gagtgatgtg atcccgcctc
    12001 agtacattgc ttccgatgct cacattgagc gcagtatcgt tggcgaaggt acagaagttt
    12061 acggtgatgt tatcaactcc gttatcggag caggcgttac cattgcaaag ggtgctgttg
    12121 ttaaggactc tattgttatg cagggaagcg tcatcggcgc aggcacatcc gttgaaaagg
    12181 ccattatcgc agaaaatgta aaagttggtt ccgatgttca gatcggtgtt ggcgaatacg
    12241 caccaagtac atatgaccag aaggtatatc agtttgacct ggctaccatc ggcgagaact
    12301 ccattatccc ggacggcgta aagatcggaa agaataccgc catagcaggc gagaccaccg
    12361 taggcgacta tcctgatgga ttactggcaa gcggaaatta cattataaag gcaggtggcg
    12421 tgaaatgaga gcaatcggta tcgtattagc aggtggtaac agcaagagga tgcgggaact
    12481 gtccagtaaa agagcagtgg ctgctatgcc ggttgcagga agctaccgca gtattgactt
    12541 tgcattaagc aatatgacaa actcccacat tcagaatgtg gctgtattta cacagtataa
    12601 ctccagatcc ttaaaccttc atctgagttc ctccaagtgg tgggatttcg gacgtaagca
    12661 gggcggactg tatgtattta caccatccat tactcctgaa aacggagact ggtaccgtgg
    12721 tacagcagat gctctttacc agaacctgac cttcttaaag aacagccatg agccatatgt
    12781 agttatcacc tctggtgacg gcgtttataa gctggattac aacaaggttc tggaatacca
    12841 tattgagaag aaagcggata ttaccgttgt ctgcaaagat atggaagagg gaacggatgt
    12901 tacccgtttc ggatgcgtta agttaaatga tgacggacgc atcactgatt ttgaagaaaa
    12961 gccaatggct tctgacgcta ccaccatttc ctgtggtatc tatgttatcc gcagaagaca
    13021 gctgatcgag ctgttagagc gctgcgcagc agaagacaga tacgacctgg taaatgatat
    13081 cctggtacgt tataagaact taaagaggat ctatgcttat aagttagaca gctactggag
    13141 caatattgca accgttgatt cttactataa gaccaacatg gatttcttaa agccggaagt
    13201 aagagattat ttcttcaaac agtatccgga tgtttatacc aagatcgatg atcttccacc
    13261 ggcaaaatac aatccaggag cagttgttag gaacagcctt gtttccagtg gatgtatttt
    13321 aaatggtacg gtagaaaact ctatcctctt taagaaggca tacgttggaa ataactgtat
    13381 catcaagaat tctattatct taaatgatgt ttatattggt gataatacag tgatcgagaa
    13441 ctgtatcgta gagagccggg ataccatccg cgccaataca acctatatcg gaacacctga
    13501 taacatcaag atcgttattg agaagaatga acgctacaca ctgtagataa gtagtaaata
    13561 gtgcggcccg tttttttctg attccttcag aaggggtgca gcacgggggc tgacggagga
    13621 agcggaagga gtattaaagc atgcaggtaa cagatgtgag agtaagacgg gtggagaaag
    13681 aagggaagat gaaagcaatc gtctccatca ccttagacaa tgaattcgtg atccacgata
    13741 tcaaagtcat tgagggagaa aagggattgt tcatcgccat gccaagccgc aaggcagcag
    13801 atggcgaata ccgcgacatc gcccatccga tcaattccgg tactagggaa atgatacaaa
    13861 gcatcatcct ggataagtat gaaagtactt tgctggagaa tgaagaagaa gcgcttgcat
    13921 aagcggcgga ccactggtga caacaggcac aatcatgtga tcaccttaaa caaaagataa
    13981 aaggcagcga cagctgcagc agggataccc tggaccagaa aatatggcag atgccatgac
    14041 tggtccgggg tatttttatg tcgctataaa tgaaagccag ttgttacatg ccttcggcac
    14101 tcccacaact gccgcccgca ttcgtaatcc gggctattgc cctgcttact catgcaggct
    14161 tggttgccaa atagtttgaa attcaaaata ttagcattgc atgtggggag tctctgtgct
    14221 atagttaggg cgtaaatgtt tattaataga cattaataga cattgtgtac aataaaatga
    14281 acgatcaccc gccagagcgg gtcgatccgt tgttatgtgt tgaaagggag ctgccgctgg
    14341 cagatctttc aagtggtttt atgaggagaa aagcaatgat ttcatacgag aatacaaaga
    14401 cagtaagaga cctggtctgg aatacgggaa gagagtatgc cggacgggta tttttacgct
    14461 atgaggaaaa tgacgtagta tatgaagtaa cttatgatga gtttgcaact cagtgccgcg
    14521 gggtagctgc ctggctgcgg gagcaggacg cggcagcggg ccacaaggta agagtaggat
    14581 taatgggaag cagcagtcac cattacctgg tatttttatt aggcgttatg gccagcggaa
    14641 atgtagtggt tcccttagat gtacagttaa atgaagagac ctttgcagat aatgtaaacc
    14701 gttctgacct ggatatttta ttttacgact gggatcatta tggcctggta gaagcagtaa
    14761 aggagaaatg tccacgcctt cgggcctgca tttccttaca gcacggaaaa catgtgccat
    14821 gttcggacca gatcttaaag ggatatgcag ggatcagtgt cgatccggtg gtttctgagg
    14881 aagactgtgc tatggtgctg tttacatcag gaacaacagg cagaggaaag ggcgttatgc
    14941 tttccaaccg caacctgatg gataataact tctgcaccac agataaagac catcctgaaa
    15001 aagaagttta cttaaatatc cttcccatcc accatgtatt ctgcatcaac ggtgatgtat
    15061 ttacggtcat gcgttatgga agcgttttgt gtc
//
LOCUS       DBMDUA010000053        15061 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2178_length_15061_cov_2.786364, whole genome shotgun sequence.
ACCESSION   DBMDUA010000053 DBMDUA010000000
VERSION     DBMDUA010000053.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 15061)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15061)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15061
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2178_length_15061_cov_2.786364"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="ACZPCL_05870"
     CDS             <1..814
                     /locus_tag="ACZPCL_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1162037.1"
                     /translation="QVSGMNIHYKFYSLEYFLESQMRAGFKTIELWAGSPHFFLSNLE
                     YDDCKKVSKMARERGLTIQVITPENCSVPYQFAAGDPDLYAKSFEYFKKGLDAGEELG
                     CKVMAVHSGRGYLNEDREEGWKRGRDMLARLADYAGTKGITLAMESLRPDETNLATTV
                     DDVKRMLTEINHPNFKAMIDTCPMGVAGETLQQWFDTLGAENIVHMHFIDGTPYGHLI
                     WGDGNHNLKNWLETVNKNGYTGLLSQEITASDYYQKPFEADCRNFKMFEPYM"
     gene            989..1858
                     /locus_tag="ACZPCL_05875"
     CDS             989..1858
                     /locus_tag="ACZPCL_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1162038.1"
                     /translation="MSEAKIIAIGDNVVDKYLSRGKMYPGGQCVNTCVYSIMNGGKPA
                     YLGKFGDDGVAEYNCKILTQLGVDFSHSRHFHGENGAARVTLKDGDRVFLGSNRGGVA
                     KEHPFDFTKEDLDYIKSFQVIYSNTNSYIYNDLSLLEETGVPIAFDFSTVWDDALFEQ
                     VCPHIKVALLSCAHLTAEEREREMKKAASYGVTLVVGTVGEDGSYALYDGEIVYAPAY
                     KAETVLDTMGAGDSYFSTLLTTLLQENGALFVGDHNHMIAQLKHAMDCGAKFAAKVCS
                     LEGAFGYGTAIID"
     gene            1962..2672
                     /locus_tag="ACZPCL_05880"
     CDS             1962..2672
                     /locus_tag="ACZPCL_05880"
                     /inference="COORDINATES: protein motif:HMM:NF019322.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1162039.1"
                     /translation="MIKNSMVPLYQQLSEKIKQQITDGKLKAGDKLMTEAEFSQHFEV
                     SRITVRKAIELLADDGFVVRKQGIGTFVAEKKLHRVVDNQIISFTEMSKMDGHVPSAE
                     LITVEWITPDGSIARKLRVDENEKVLRIVRLRKNDDVPVMIEDSYYPKRMEFLLKENL
                     LGSTYEFFHNHGLIPTHSVKTVEICYATPEEAQRLGVPEKHALLLQKDEVFDQDNEVL
                     HYSKLLTNPQRYRLTIVT"
     gene            2966..4324
                     /locus_tag="ACZPCL_05885"
     CDS             2966..4324
                     /locus_tag="ACZPCL_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1162040.1"
                     /translation="MKKQLKKTVSLGLAAVMALGLTACGGGSSSSDSTTAAAGSGENK
                     TEAAADSGNSGDVKVIKLFHRFPDDPCNSFIESKLAEYESLHPDIKFEVTSAQNQPYK
                     EKIKVVVGSDECPDIFFSFCGEFSERFIREGLLLDLTPYMEADPDWKASLMETQMNEY
                     TTADGMVYGIPFRLDAKEFFYNKDIFNELGLEVPETWDQFIDVLDKIKASGMDPIAEG
                     NQDKWPSAHYIGALNEQLVDDETRAKDYNPKTGEFTDPGYAEALEKYQQLVSYMNTGV
                     NGMTHDMARLGFTQGQNAILFAELVEITNVKQENPDLNWGMFKFPTIEGAKGNPNLVS
                     GAPEGFAISSKTKYPDECVEFLKWFLGAEVGAQQAEQVGWFNASLGLDKTLTDESLKS
                     AYDVISNAKKMDIWFDNALYSTVCDEYLTQISDITNGDVTPTDAMAKIQKIAAEAQKL
                     VE"
     gene            4405..5286
                     /locus_tag="ACZPCL_05890"
     CDS             4405..5286
                     /locus_tag="ACZPCL_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1162041.1"
                     /translation="MKKKTKLTPYLYVIPGLLMVLGFVYIPIIVNLVYSFFRLSSYSS
                     SMKFVGLDNFRRLFTNDTFPIMIRNNVYYCVISLIVQVGFGTFLAFLLESRLASDRFK
                     SICRNIYFLPALVSLTAVGMMFNFIYNPQLGLLNTFLKHIGLASMQQTWLGDKNLAIF
                     CIIAMSQWQFTGYITLLMVVAFQNVSRDYIEAAVIDGAGPIRRAVSIILPLAKEQLIV
                     CSIITVIGAFKLFTEVYSTTVGGPGNASQVLGLFLYQNAFLHDDMGMAAATGVLIFVI
                     TVTASIFQLKVSRSGEI"
     gene            5299..6138
                     /locus_tag="ACZPCL_05895"
     CDS             5299..6138
                     /locus_tag="ACZPCL_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1162042.1"
                     /translation="MKDKYVLKTRICSVLLHVFLLLMVAIILLPVCWLVLNSLKTNQE
                     MFLNSLALPKKWMFVNYATAWGKGLLFYFKNSIIVSAISILCILLLSSMLAYGLTRFS
                     LPGSGFVFFLILGGMALSEQVALVPLYKILQALKLYNTRAALILPYIAFRIPFTMFLM
                     RAYFISIPKELEEAAIVDGCNSFQRFTQIILPISKPVMASCAIVNLNNVWNEFLFANV
                     FLENKKLMTIPLGLMTFQGDLKSDYVVMLAGILIASLPMVLLFLCMSKQFVRGLTSGA
                     VKG"
     gene            6321..7130
                     /locus_tag="ACZPCL_05900"
     CDS             6321..7130
                     /locus_tag="ACZPCL_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HIR1162043.1"
                     /translation="MLTADYHMHSVSPDARVPMEDMCQAAIDKGLTEVALTDHYEFYA
                     HGIHRKFFHEEYLKMYWDSLERCRERFAGQLTIKSGMEFGQLHLTREEAFNIIRNHPF
                     DYLIGSVHKIENVDVSKMEYTDVTVPQITESYYRHLIELSAYGEYDCLGHIDLIKRHL
                     VRCGFPVEYEKYEGYIDEILKNVISRGKGIEVNTSGIRQGAGEPMPGLRTLKRYKELG
                     GEIVTVGSDSHKPADVAADFEEAAKLLVQAGFDHVTAYERRRPVPGCNLIK"
     gene            complement(7334..8302)
                     /locus_tag="ACZPCL_05905"
     CDS             complement(7334..8302)
                     /locus_tag="ACZPCL_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1162044.1"
                     /translation="MTIKEIVASIKAKQPEIKTVYFVGCGASQSDLFPAKYFLENNAK
                     KLRTSIYTANNFNYSTPAGVDDTAIVITCSLSGNTPETVAATKLAVEKGAHVVAVTHK
                     ADSALAQNGQYQIIHGFYESYGAKMEKPARVLELACEILNEYEGYEHYDDMQDGLSKI
                     FDLINDSCKLFRSTAKKFAEDHYNAPILYVMSSGATQYTAYSFSMFLMMEMQWLPSST
                     FHTGEFFHGPFEMADENAHYLLMMNDGPTRHLDARALTFLQRMNAKYTVVDAKDFGLS
                     SHIKSTVVEYFNPLMIGGVTRLLGEEMAIKRDHPLTMRRYMWKLEY"
     gene            complement(8509..10206)
                     /locus_tag="ACZPCL_05910"
     CDS             complement(8509..10206)
                     /locus_tag="ACZPCL_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1162045.1"
                     /translation="MNLKISIGSQDFAFLREHNCFLVDKTDFIKKWWESGDAVTLITR
                     PRRFGKTLNMSMLNCFFSRQYEHRGDLFEGLNIWSDLKYRNIQGSYPVIYLSFADVKQ
                     DCYGDTVQKIRHIIGDTYRQHRYLLDALCFTEAEKHQFTKVMNCEMDDVTAQDALKNL
                     SYYLHHYWRKRTILLLDEYDTPMQEAYVHGYWNEFINFIRGLFNSALKTNPYLERAVM
                     TGITRVSKESVFSDLNNLKVITITSEEYADCFGFTEEEVFAALKQTGLEDKKTDVKEW
                     YDGFIFGSLHDIYNPWSITNYLDTRKLQPFWASTSSNSLVSRLIQTASGEIKERMEEL
                     LQGRQIVVTFDEQIVFDQLGRNENAIWSLLLASGYLKAEQVEYRGLIREPWYHLSITN
                     LETASMFSGMFKGWFGMTQTSYNRFVKALFQGDVDAMNYYMNEVSVATFSFFDTGKEG
                     GKDSEPERFYHGFVLGLLADQAEQYEIRSNRESGFGRYDVMMIPKNQKNTDGLAFILE
                     FKVRNPGKEKSLEETVQTALEQIETKQYDAELLARGIEKERIRHYGFAFDGKQVLIG"
     gene            complement(10241..11017)
                     /locus_tag="ACZPCL_05915"
     CDS             complement(10241..11017)
                     /locus_tag="ACZPCL_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR1162046.1"
                     /translation="MKVDAGKIVELVRKMKPLFADSSRVSQVKEKGTCDYVTQVDIQV
                     QKLVKEGLYSMYPQVQFMGEEQDNSAIDRSGDFWILDPVDGTTNLIHDFRHSTLSLAY
                     AEAGEVVLGIVYQPYTDELFIAKKGEGAFLNGDPIHVSGAETLSESLVTIGTSPYYHE
                     LADWNFKVFKEVFLSCQDIRRLGSAALDMAYVACGRVDAFFEAILQPWDFSAAKLLIE
                     EAGGKVTDFAGKELPVGMPGAVLAGNGKINEELLRILSEK"
     gene            complement(11280..12554)
                     /gene="purD"
                     /locus_tag="ACZPCL_05920"
     CDS             complement(11280..12554)
                     /gene="purD"
                     /locus_tag="ACZPCL_05920"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR1162047.1"
                     /translation="MKVLVIGGGGREHAICWKLSKSPKVDELYCAPGNAGIAEVAKCV
                     DIGVMDFEKMADFAKKEAFDLVVVGPDDPLAGGIVDVLEEKGLRVFGPRKNAAILEGS
                     KAFSKDLMKKYGIPTAAYETFDTPEAALNYLETAPVPIVLKADGLALGKGVLICNTRE
                     EAKEGVKTLMLDKQFGHAGDRIVVEQFMTGREVSVLSFVDGKTIKIMTSAQDHKRAKD
                     GDQGLNTGGMGTFSPSPFYTPEVDEFCKKHIYQATVDAMKAEGREFKGIIFFGLMLTA
                     DGPKVLEYNARFGDPETQVVLPRMKNDIVDVFEACVDGTLDQIDLQFEDNAAVCVVLA
                     SDGYPEHYEKGKKITGLENFKDKDGYYVFHAGSKFDAEGNILTNGGRVLGVTATGKDL
                     KEARANAYKATEWVNFENKYMRHDIGHAIDEA"
     gene            complement(12565..13158)
                     /gene="purN"
                     /locus_tag="ACZPCL_05925"
     CDS             complement(12565..13158)
                     /gene="purN"
                     /locus_tag="ACZPCL_05925"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR1162048.1"
                     /translation="MLRVGVMVSGGGTNLQAILDAIDSGKITNAKVEVVISNNPGAYA
                     LERARNHGIEAVCISPKSFENRAAFNEAFLKKTDEYDLDLIVLAGFLVTIPEEMIRKY
                     RNKIINIHPSLIPSFCGVGYYGLKVHEAALARGVKVTGATVHYVDEGVDSGPILLQKA
                     VEVQPGDTPQVLQRRVMEQAEWIILPEAVNKIANGLI"
     gene            complement(13152..14183)
                     /gene="purM"
                     /locus_tag="ACZPCL_05930"
     CDS             complement(13152..14183)
                     /gene="purM"
                     /locus_tag="ACZPCL_05930"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR1162049.1"
                     /translation="MDYKKAGVDIEAGYKSVELMKEHVKKTMRAEVLGGLGGFSGAFS
                     LAKIKEMAEPVLLSGTDGCGTKVKLAIIMDKHDTIGIDAVAMCVNDIACAGGEPLFFL
                     DYIACGKNYPEKIADIVKGVAEGCLQSEAALIGGETAEHPGLMPEEDYDLAGFAVGVC
                     DKKEMITGEKLEAGDVLIGMASTGVHSNGFSLVRKVFEKEMNKEGLNTYYDSLGKTLG
                     EALLAPTRIYVKALKNIKKSGVTVKACSHITGGGFYENVPRMLKDGVRAVIRKDSYPV
                     PPIFKLLAEKGKIEEKMMYNTFNMGLGMVVAVDEADVDKTMAAIKAAGDQPYVVGTIE
                     NGEKGVTLC"
     gene            complement(14272..14784)
                     /gene="purE"
                     /locus_tag="ACZPCL_05935"
     CDS             complement(14272..14784)
                     /gene="purE"
                     /locus_tag="ACZPCL_05935"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR1162050.1"
                     /translation="MAKVGIVMGSDSDMPVMAKAAEILDKFGIDYEMTIISAHREPDL
                     FFKWAKAAEEKGFKVIIAGAGKAAHLPGMCAAIFPLPVIGIPMKTSDLGGVDSLYSIV
                     QMPSGIPVATVAINGGANAGILAAKILATSDEELLGKLKAYSENLKEDVEKKAEKLEK
                     IGYKEYMAQK"
BASE COUNT         4171 a         3632 c         2942 g         4316 t
ORIGIN      
        1 ccaggtatcc ggcatgaaca tccattataa attctattct ctggaatatt ttctggaatc
       61 ccagatgcgg gccggtttta agaccatcga attatgggct ggttctcctc attttttctt
      121 aagcaatctg gaatatgatg actgtaaaaa agtaagcaaa atggcaagag aacgtggtct
      181 taccatccag gtcatcaccc cggaaaactg ttctgtcccc tatcagtttg cagccggaga
      241 cccggatctg tatgccaaaa gttttgaata tttcaaaaaa ggcctggatg ccggtgaaga
      301 acttggctgt aaggtcatgg ctgtacactc cggacgggga tatttaaacg aagaccggga
      361 agaaggctgg aaacggggcc gcgacatgtt ggcacggctg gctgattatg ccggcacaaa
      421 gggcattacc cttgctatgg aatcccttcg cccggatgag accaatctgg caaccactgt
      481 tgatgatgta aagcggatgc ttacggaaat aaaccatcct aattttaaag ccatgatcga
      541 cacctgtcca atgggcgtgg caggcgagac tttacagcag tggtttgata ccttaggagc
      601 cgagaacatt gtacacatgc acttcataga cggaactccc tacggacatc tgatctgggg
      661 tgacggaaat cacaacctga aaaactggct tgaaactgta aataaaaacg gctacaccgg
      721 acttttaagc caggagatca ctgcttccga ctattatcag aagccatttg aggctgactg
      781 ccgaaacttc aaaatgtttg aaccttatat gtaatgatac cagggtcaaa aaatcgtaaa
      841 tccgacgtaa gcttgcaaga aaaacctccg gtaatataca aactcattga caagatgtta
      901 tatattgtta tatattgtat ctaacagatt gttgttgtcc tacggacatc cgtcaggaaa
      961 tatgagcaaa aaaccggagg tttttaatat gagtgaagca aaaatcattg ccattggtga
     1021 taatgtagtg gataaatact tatccagagg aaaaatgtat ccaggaggac agtgtgtaaa
     1081 cacatgtgtc tattccatta tgaacggcgg aaagccagca tatcttggta aatttggtga
     1141 tgacggcgtt gctgaataca actgcaaaat cttaacccag ttaggcgtag acttcagcca
     1201 ttccagacat ttccacggtg aaaacggtgc agcccgcgtt accttaaagg acggtgatcg
     1261 tgtattctta ggctccaacc gcggcggtgt tgccaaagaa catccatttg actttacaaa
     1321 agaagatctg gactatatca agtccttcca ggtgatctac tccaatacaa acagctatat
     1381 ctataatgat ctatctttat tagaagagac aggtgtacct attgcctttg atttctctac
     1441 tgtatgggac gatgccctgt ttgaacaagt gtgtcctcat atcaaagtag ctcttctttc
     1501 ctgcgcacat ttaactgcag aggaaagaga gcgtgaaatg aaaaaagcgg cctcctacgg
     1561 ggtaacctta gtagtaggta ccgtaggaga agacggctcc tatgcacttt atgacggtga
     1621 gatcgtctat gcacctgctt ataaagcaga aaccgtatta gataccatgg gcgccggaga
     1681 ctcctatttc tccactctgc tcactaccct gcttcaggaa aatggagctc tttttgtcgg
     1741 tgaccataac catatgatcg cccagctaaa gcatgccatg gactgtggag caaaatttgc
     1801 agcgaaggtc tgcagcttag agggggcttt cggctacgga actgctatta tcgattaaca
     1861 ttctcccagt ttcggaattt ttcacgcacc tgtttttata gccatgtcgt atttaagcaa
     1921 aacaatataa atgtaatgta ttaggagtga aataaatatt catgataaaa aattctatgg
     1981 tgcctcttta ccaacagctg tccgaaaaga tcaaacagca gatcacagac ggtaaattaa
     2041 aagccgggga caagctgatg acagaggcag aattcagcca gcattttgaa gtaagccgca
     2101 ttactgtgcg caaagccatt gaactgctgg cagatgacgg tttcgtagta cgcaaacagg
     2161 gcatcgggac ctttgtggca gaaaagaaac tgcaccgtgt tgtagacaac cagatcataa
     2221 gctttacgga aatgagcaaa atggacgggc atgtgccatc tgcagaactg atcactgtag
     2281 aatggataac ccctgacggc tccattgcca gaaaacttcg tgtagatgaa aacgaaaaag
     2341 tgctgcgtat agtccgtctg cgtaaaaatg acgatgtgcc ggtgatgatc gaggacagct
     2401 actatccaaa gcggatggaa tttctgttaa aggaaaatct tttaggttct acctatgagt
     2461 ttttccataa ccacggactg atcccaaccc attctgttaa aacagtagag atctgctatg
     2521 ccacaccgga agaagcacag cgtctggggg ttccggaaaa acatgccctc ctgctgcaaa
     2581 aggatgaggt tttcgatcag gataatgaag tgctgcatta cagcaagctg cttaccaacc
     2641 cccagagata ccgtcttacg attgttacgt aagttcatag tacaaaatgc tatacctaat
     2701 attcttcagg tgagacaata caactataaa gaggaacttt aaaaacaacg gcgtttttta
     2761 tgaaataatt ttattttctt aagaaacgtc gtcatttttt ctcaaaaaag taccggttag
     2821 ttaaaacttt atcaacaaaa gttacaaaaa cattaaaaaa acagttgcaa gtcatataac
     2881 aatgtggtat gccttgtact aaggtgttat gtgttgtatc aatacaacag agacaccgga
     2941 aactgaggac cgaaaggaga tcattatgaa aaaacaactg aaaaagaccg tgagccttgg
     3001 acttgcagca gtaatggcac tgggccttac cgcatgcgga ggcggcagtt cctcttctga
     3061 cagcaccaca gccgcagcag gcagtggaga aaacaaaaca gaagcagctg ctgattccgg
     3121 aaacagcggt gacgtaaaag tcatcaaact gttccatcgt ttccctgatg atccatgcaa
     3181 cagcttcatt gaaagcaaac ttgctgagta cgaatccctt catccagata tcaagtttga
     3241 agtgaccagc gcacagaacc agccatacaa agagaagatc aaagtcgtag ttggttccga
     3301 tgagtgccca gatatcttct tcagcttctg cggtgagttc tctgagagat ttatccgtga
     3361 aggcctgtta ttagacttaa ctccatatat ggaagcagat cctgactgga aagcttctct
     3421 gatggaaaca cagatgaatg aatacacgac ggcagatggc atggtttatg gtatcccatt
     3481 ccgtctggat gcaaaagaat tcttctacaa caaagatatc ttcaacgagt taggactgga
     3541 agttcctgag acatgggatc agttcatcga tgttcttgac aagatcaaag catccggtat
     3601 ggatcctatc gcagagggaa atcaggacaa gtggccatct gctcactaca tcggtgcatt
     3661 aaatgagcag ttagtagatg atgagaccag agcaaaggat tacaatccta agaccggtga
     3721 atttaccgat ccaggatatg cagaagctct tgaaaaatac cagcagttag tatcctacat
     3781 gaacacaggt gtaaatggta tgacccatga tatggcccgt ttaggcttta cccagggcca
     3841 gaacgctatc ctctttgcag agttagttga gatcaccaat gttaagcagg aaaacccaga
     3901 tctgaactgg ggaatgttca agttcccaac catcgaaggt gctaagggta atccaaatct
     3961 ggtatccggc gctcctgaag gatttgcgat ctcctctaag accaagtacc cagatgagtg
     4021 cgtagagttc ttaaaatggt tccttggtgc tgaagttggt gcacagcagg ctgagcaggt
     4081 tggctggttc aacgcttccc ttggtctgga caagacttta accgatgagt ccttaaaaag
     4141 tgcttacgat gtaatctcca acgctaagaa gatggacatc tggtttgata acgctctgta
     4201 ctccacagtt tgcgacgagt atctgaccca gatctccgac atcacaaatg gtgacgtaac
     4261 cccaactgac gcaatggcta agatccagaa gatagctgct gaagcccaga aactggttga
     4321 atagttttcg ttaaagtttt ataagaaatc cacgataggg agcagcatct gctccctatc
     4381 tttaaagaaa aggatggtgt caggatgaaa aagaaaacaa aactcacacc gtatttatat
     4441 gtgatccccg gccttttaat ggtccttggc tttgtttata tccctattat cgttaacctt
     4501 gtatacagct ttttccgtct ttcttcctac tcttcttcca tgaagtttgt aggacttgac
     4561 aatttccgca gattatttac aaatgataca ttccctatca tgatccggaa caatgtgtat
     4621 tactgcgtga tctccctgat cgtgcaggtt ggatttggaa cattcctggc tttcctgtta
     4681 gagagcagac ttgccagtga ccgctttaaa tcgatttgca gaaacatcta tttccttccg
     4741 gcactggttt ccctgactgc cgttggtatg atgtttaact ttatctacaa cccgcagctt
     4801 ggtcttttaa acacattttt aaagcacatc ggacttgcga gcatgcagca gacctggtta
     4861 ggtgataaaa acctggctat cttctgtatc atcgcaatga gccagtggca gttcaccggt
     4921 tacattaccc tgttaatggt agttgctttc cagaacgtgt ccagagacta cattgaagct
     4981 gctgttattg acggcgcagg tcccatccgc cgcgcagtaa gcattatcct tccccttgca
     5041 aaggaacagc tgattgtctg ctccattatc actgtgatcg gtgcattcaa gctgtttacc
     5101 gaggtttact ccaccactgt cggtggtcct ggaaatgctt cccaggtact tggcttattc
     5161 ctgtatcaga acgcattctt acatgatgat atgggtatgg ctgctgcaac tggtgttctt
     5221 atttttgtca ttaccgtaac cgcctctatc ttccagctga aggtgagccg ttccggagag
     5281 atataaggag gttttggtat gaaagacaaa tatgtattaa aaacccggat ttgttccgta
     5341 ctgctccatg tatttcttct gttaatggta gctatcatcc tgcttcctgt atgctggctg
     5401 gtgttaaact ccttaaagac aaaccaggaa atgttcttaa actccctggc tcttccaaaa
     5461 aaatggatgt ttgtcaacta cgcaactgca tggggcaaag gacttctctt ctactttaag
     5521 aactccatta tcgtcagtgc tatttccatt ctgtgcatcc tgcttttaag ctctatgctg
     5581 gcatatggac tgacccgttt ttcccttccc ggaagcggtt tcgtattctt ccttatttta
     5641 ggcggtatgg ccctttccga acaggttgct ttagtacctc tgtacaagat cttacaggca
     5701 ttaaagcttt acaatacccg tgcagcgctg atccttccgt acatagcatt ccgtatccca
     5761 tttaccatgt ttttaatgag agcttacttc atctccattc caaaggaatt agaggaagca
     5821 gcgatcgtag acggatgcaa cagcttccag cgcttcacac agatcatcct tcctatcagt
     5881 aagccggtta tggcttcctg tgccattgta aacttaaaca atgtatggaa tgaattctta
     5941 tttgcaaatg tattccttga gaataaaaaa ttaatgacca tcccgttagg cctgatgacc
     6001 ttccagggtg acttaaaatc cgactacgtt gtaatgttag ccggtatcct gatcgcctct
     6061 ttaccaatgg tactcctgtt cctttgcatg tccaaacagt ttgtacgcgg acttacatcc
     6121 ggagcagtaa aaggctgatc tcttcaattt tcattatgat atcaaaaaga ctgctctttc
     6181 ggtccacagg ccgtaatgag cagtcttttt tatgtactag gaaattccgt tggaactccc
     6241 tagtacacaa aaaggcactt cgtgccaacg atgcgcactc gcgcaattct aaaaaataca
     6301 taatatcagg aggaattcat atgcttacag ccgattatca catgcacagc gtttcacctg
     6361 acgccagagt ccctatggag gatatgtgtc aggcagcaat agacaaaggc cttacagaag
     6421 tagctcttac agatcactat gaattttatg cccatggcat ccacagaaag tttttccacg
     6481 aagaatacct gaagatgtac tgggacagct tagaacgctg cagagagcgt tttgcaggcc
     6541 agctgaccat caaaagcggt atggagttcg gacagctcca tttaacccgt gaagaggctt
     6601 ttaatattat aagaaaccac ccttttgact atctcatcgg ttctgtccac aagattgaaa
     6661 atgtagatgt gagcaaaatg gaatataccg atgtcaccgt tccacagatc acagaaagct
     6721 attaccgtca tctcatagaa ctgtctgcct acggcgaata cgactgcctg ggacatatcg
     6781 atctgatcaa acgccatctg gtgcgctgcg gcttcccggt agaatatgaa aaatatgaag
     6841 gctatattga tgagatcctt aaaaatgtga tctcccgcgg aaaaggcatc gaagtcaata
     6901 catccggcat ccgccagggc gccggtgaac caatgccggg acttcgtaca ttaaaacgat
     6961 ataaagagct tggaggcgag atcgtcactg taggttctga ctcccataag cccgccgatg
     7021 tagctgctga ctttgaagaa gccgcaaagc ttctggtcca ggccggattt gaccatgtaa
     7081 ctgcctacga gcgccgcaga ccggttccgg gatgcaatct aataaagtaa tcatgtatta
     7141 tccccaaatg tcttgctgtg gcttctggac gttccagtaa gcttcttccc acaaaatcca
     7201 ccaaaacagc atcgtttctt tccccgccca gagggcgggg aatacacact ttctccccac
     7261 aaaaatgccc cggaagcatc gcttccgagg cattttcaca tttatcatat tcttttagag
     7321 agtacaccag aaattagtat tccagcttcc acatatatct tctcattgtc agaggatgat
     7381 cacgcttgat agccatttcc tcacctaaca gtctggtaac gccgccgatc atcagtgggt
     7441 tgaagtattc aacaactgtg ctcttgatat gggagcttaa accgaagtcc ttagcatcaa
     7501 ctacagtgta cttagcgttc atacgctgta agaaggtcag tgctcttgca tccagatgac
     7561 gggtaggacc atcgttcatc attaacaggt agtgagcgtt ctcatcagcc atctcgaatg
     7621 ggccatggaa gaattcgccg gtatggaagg tgctggatgg cagccactgc atttccatca
     7681 ttaagaacat agagaaggaa taagcggtgt actgggttgc accggaactc attacataca
     7741 ggataggtgc attgtaatga tcctcagcga acttcttagc agtagaacgg aacagcttgc
     7801 aggaatcatt gatcagatca aagatcttgc ttaaaccatc ctgcatatca tcatagtgct
     7861 catagccttc gtactcattt aaaatctcgc aagccagttc cagaactctt gcaggctttt
     7921 ccatctttgc accatagctc tcatagaagc catggatgat ctggtactga ccattctgag
     7981 ccagagctga atctgcctta tgagttacag ctactacatg agcacccttt tcaacagcca
     8041 gcttggtagc tgctacagtc tcaggtgtat ttccgcttaa ggagcaggtg ataacgatag
     8101 cggtatcatc tacaccagct ggagtagagt aattaaagtt atttgcagta tagatacttg
     8161 tacgaagctt cttagcgttg ttctccagga aatattttgc agggaacaga tcagactgtg
     8221 aagcgccgca gcctacaaag tatacggtct tgatttctgg ctgttttgcc ttgatggaag
     8281 caacgatctc tttgattgtc ataattggta tcctcctgaa ttttgttctt ttataatatc
     8341 tgaatacatt atataacaac atatgacaat agtcaataca taattgaaat tctttaaaat
     8401 cttaagttgc agttgaacta taacgatctc taccttaact cttaaaaaga accatgggct
     8461 gtacagatat cagaacccat cccaaaatac aaaaacagat actgaccatc atccgatcag
     8521 tacctgcttt ccatcaaatg caaatccata atgccggatc cgctcttttt ctattcctct
     8581 tgcaagtaat tccgcatcat actgctttgt ttctatctgc tctaatgccg tctgcacagt
     8641 ttcctccaga gatttctctt ttcctggatt tctcacttta aactccagaa taaaagccaa
     8701 accatctgta ttcttctgat tctttgggat catcattaca tcgtatcgtc caaacccact
     8761 ttcccggttg gaccggattt catactgttc tgcctggtct gcaagaagtc ccagaacaaa
     8821 accatgataa aaacgttctg gctcgctatc tttaccccct tcttttcctg tatcaaaaaa
     8881 actgaatgtt gcaacggaca cctcattcat atagtaattc atagcatcca catcgccctg
     8941 gaacaacgct tttacaaatc gattatagct ggtctgtgtc atgccaaacc agcccttaaa
     9001 catgcctgaa aacatactgg ccgtttccag atttgtgata ctcaaatgat accatggttc
     9061 ccggatcagg ccacgatatt ctacctgttc cgcctttaaa tacccactgg ctaaaagcag
     9121 actccagatt gcattctcat tccttccaag ctgatcaaat acaatctgtt catcaaatgt
     9181 gaccacgatc tgtcgtcctt gtaaaagttc ttccatcctt tccttaatct ccccggaagc
     9241 agtctggatc agacggctta ccagactgtt agaacttgtt gatgcccaga acggctgcag
     9301 ctttctggtg tccagataat tggtgataga ccatggattg taaatatcat gcaggcttcc
     9361 aaagataaag ccatcatacc attcttttac atctgtcttt ttatcctcca gcccagtctg
     9421 tttcagagct gcaaatacct cctcttccgt aaaaccaaaa caatccgcat actcttctga
     9481 agtgatcgtt atgactttca gattattcag atctgaaaat acagattcct tggatacccg
     9541 ggtaattccc gtcataacag cacgttccag atatggattc gtttttaaag cagaattaaa
     9601 taagccgcgg ataaaattaa taaattcatt ccaatatcca tgcacatacg cctcctgcat
     9661 gggcgtatca tactcgtcaa gcagaaggat agtacgcttt ctccagtaat gatgcaaata
     9721 ataagataaa tttttcaaag catcctgagc tgtcacatca tccatctcgc aattcatcac
     9781 ttttgtaaac tgatgttttt ctgcttctgt aaaacataat gcatccagaa gatagcggtg
     9841 ctggcggtaa gtgtcaccaa taatatgtcg gattttctgc actgtatctc cataacaatc
     9901 ctgttttaca tctgcaaaac ttaaataaat aacaggataa cttccctgga tattccggta
     9961 tttcagatca gaccagatat taagtccttc aaacagatcc cctctgtgtt catactgccg
    10021 ggaaaaaaag cagttcaaca tactcatatt taacgttttt ccaaaccggc gcggacgggt
    10081 gatcagcgta actgcatccc ctgactccca ccatttctta ataaaatcag ttttatccac
    10141 caaaaagcag ttatgctccc gcaaaaaagc gaaatcctga cttccgatac tgattttcaa
    10201 gttcatatgt tcacctgccc ttccttctct attaccctct tcacttctca cttaatatcc
    10261 tgagcaattc ctcatttatc ttcccattac cagccagaac agcccccggc attcccacag
    10321 gcagttcctt tcctgcaaaa tccgttactt ttcccccggc ttcctcgatg agaagctttg
    10381 ctgctgaaaa atcccagggc tgtaagattg cctcaaagaa cgcatccact ctcccacagg
    10441 ctacatatgc catgtcaaga gccgcggagc ctaaacgacg gatatcctga caggacagaa
    10501 atacttcttt aaatacctta aaattccagt ctgccagttc atgataataa ggacttgttc
    10561 caatagtaac aaggctttct gacaatgttt ctgcaccgct tacatggatg ggatcaccgt
    10621 ttaaaaatgc cccttctcct tttttggcaa taaacagttc atctgtataa ggctgataca
    10681 caattccaag caccacctca ccagcctctg cataagccag tgaaagggta ctgtgacgga
    10741 aatcatggat cagattagta gtcccatcta ccggatccag gatccagaaa tccccgctcc
    10801 tgtcaatagc actgttatcc tgctcttctc ccataaactg tacctgtgga tacatagaat
    10861 acaagccctc ttttaccagc ttctgcacct ggatatccac ctgagtcaca taatcacagg
    10921 ttcctttttc ttttacctgg gatacgcggc tgctgtctgc aaacagcggc ttcatcttcc
    10981 ttactaactc aactattttt cctgcatcca ccttcattta tcgtacctcc accttttaga
    11041 ccaaatatct tttccaataa ttaatggatg attttattat aatagttttc agggaaaagt
    11101 ccagcatcca gatacctttt aaataccttc atttttttgt acatggcatt tttattaact
    11161 gaactgtaat gcacccacat aaaacagata tttacaaaaa caccggctat tttttagccg
    11221 gtgttcctac actttgcagt atattaaaac gatttgattt ccacattcac ccctggaaat
    11281 cacgcctcat caatagcatg accaatatca tgtctcatgt atttgttctc aaaattgacc
    11341 cattccgttg ccttataagc attggctctg gcctctttta agtcttttcc tgtagctgtc
    11401 actcccagga cacggccacc gtttgtcaga atatttccct ctgcatcaaa cttgctgccg
    11461 gcatggaata cataatagcc atctttatcc ttaaagttct caagacctgt gatcttctta
    11521 cccttttcat aatgctccgg atagccatca gaagccagca ctacgcacac agccgcatta
    11581 tcctcaaact gcaggtcgat ctgatctaaa gtgccgtcta cacatgcctc aaatacatct
    11641 acaatgtcat ttttcattct cggaagcact acctgggtct ccggatcgcc gaaacgtgca
    11701 ttgtattcca gaacctttgg tccatccgct gtgagcatta aaccaaagaa gataattccc
    11761 ttaaactcac ggccttctgc cttcatggca tctactgtcg cctggtagat atgcttcttg
    11821 cagaattcat ctacctctgg agtatagaat gggcttggtg aaaatgttcc cataccgcct
    11881 gtatttaagc cctgatctcc gtcctttgca cgcttgtggt cctgggcgga agtcatgatc
    11941 ttaatggtct ttccatctac aaaggacaat acagaaacct cacggccggt cataaactgc
    12001 tctactacaa tacggtcgcc agcatgtcca aactgcttat ctaacatgag agtcttaacg
    12061 ccttcttttg cctcttccct tgtattacaa atgagaacgc cttttcctaa agccagaccg
    12121 tcagccttta aaacgatcgg taccggtgct gtttccagat aattaagggc agcttccggt
    12181 gtatcaaaag tctcataagc tgctgtcgga atgccgtatt tcttcatcag atccttggaa
    12241 aatgccttgg atccctctaa gatcgctgca ttctttcttg gtccgaacac acgcaatccc
    12301 ttttcttcca gtacatctac gataccacct gccagtggat catccggtcc cactactacc
    12361 aggtcaaagg cttctttctt tgcgaaatca gccatttttt caaaatccat tacaccgata
    12421 tccacgcatt ttgcaacctc tgcaatacct gcatttcccg gtgcacagta taattcatct
    12481 actttcgggc ttttagataa cttccagcag atcgcatgct ctctgccgcc gcctccgata
    12541 accagtactt tcatatcttt cctcctaaat caagccattg gcgatcttgt ttaccgcctc
    12601 tggaagaatg atccactcag cctgttccat gacacgtctc tgcagtacct gaggagtatc
    12661 accaggctgc acctccactg ccttctgaag caggatcgga ccggagtcta ctccttcatc
    12721 cacatagtgg accgtagcgc ctgtaacctt tacacctctt gccagagctg cctcatggac
    12781 ctttaagcca taatagccta cgccgcagaa agacgggatc agggaaggat ggatgttgat
    12841 gatcttattt ctgtatttac ggatcatctc ctccgggatg gttactaaaa atcctgccag
    12901 cacgatcaga tccaggtcat attcatctgt tttctttaaa aatgcctcat taaaggctgc
    12961 tctgttttca aagcttttcg gtgaaatgca aacagcttcg atgccatggt ttctggcacg
    13021 ttccagtgca taagcccctg gattgttgct gatgactact tccacctttg catttgtgat
    13081 ctttccactg tcaatggcat ctaagatcgc ctgaagattg gttccgccgc cggataccat
    13141 tacgccaact cttaacataa agtcacgcct ttctctccat tttcaatggt tcctactaca
    13201 tatggctggt ctcctgctgc cttgatagct gccatggtct tatccacatc tgcctcgtcc
    13261 acagctacta ccatgccaag tcccatgtta aatgtattat acatcatttt ctcttcgatc
    13321 ttgccttttt cagcaagaag tttgaaaatc ggaggtaccg gatagctgtc cttgcggatc
    13381 acagcacgga caccgtcctt taacatacgc ggaacattct catagaaacc accacctgta
    13441 atatggctgc atgcctttac ggtcacgccg gactttttaa tgttctttaa tgcctttacg
    13501 taaattctgg ttggtgcaag aagtgcttcg cctaaggtct ttccaaggct gtcataatag
    13561 gtatttaaac cttctttatt catttccttc tcaaatacct tacgtaccag agagaagcca
    13621 ttggaatgta cgccggtaga agccataccg atcagcacat ctcctgcttc cagtttctca
    13681 cctgtgatca tttctttctt gtcgcagaca cctactgcaa aaccagccag gtcataatcc
    13741 tcttccggca tcagtcccgg atgctctgct gtctcaccgc cgatgagagc tgcctcagac
    13801 tgtaaacagc cttctgccac gcccttaacg atatcagcga tcttttccgg ataattcttt
    13861 ccgcaggcaa tatagtccag gaagaataat ggctcgccgc ctgcgcaggc aatatcattg
    13921 acacacatag ccactgcgtc aataccaatg gtatcatgct tgtccatgat aatagccagt
    13981 tttaccttgg ttccgcagcc gtctgtaccg gataataata ccggctctgc catttccttg
    14041 atctttgcta aagaaaaggc gccggagaaa ccgccaagtc cgcctaatac ttctgcgcgc
    14101 atggtctttt ttacatgctc tttcattaat tcaactgatt tataacctgc ctcaatatca
    14161 actccggctt tcttgtaatc catcatcttc ttatcctccg aatttgtcct gtctatacct
    14221 tctgtaatgt tacttttcta atgttatttt ttttatatta ttcctgtaat gttatttctg
    14281 tgccatatac tctttgtatc cgatcttttc cagcttctca gcctttttct ctacgtcttc
    14341 ttttaaattc tcagaatatg cttttaactt tcctaacagt tcctcatcag atgttgcaag
    14401 gatctttgct gcaaggatac ctgcattggc accgccgtta attgcaacgg ttgcaaccgg
    14461 gataccgctt ggcatctgta cgatggaata cagagagtct acaccgccca gatcagaagt
    14521 cttcatcggg ataccgatta cagggagtgg aaagatagct gcacacattc ctggaaggtg
    14581 agctgctttt ccagcacctg caatgatcac tttaaaacct ttttcctctg cagcctttgc
    14641 ccacttaaaa aacagatccg gttctctgtg ggcggaaata attgtcatct cataatcgat
    14701 tccaaacttg tccaggatct ctgccgcttt cgccataacc ggcatatctg agtcgctgcc
    14761 cattacaatt cctaccttag ccatctttca ttctcctttt gaataatata tattagattt
    14821 actaatatat atgttttgta tatttatctt ttctaataat tataatacta agaaataaac
    14881 ccgcagtcaa tagtaaaaac gcaaaaaagc aaatcaaaaa aagccgcaca ctgctttttt
    14941 tgccagtgtg cggctcttat cgggagtttg acacctttcc gaatgaaaaa cagcgtataa
    15001 gccgcatgaa tgcttatatt tttttgtcaa atttgttgtt tttatgtata ggctttttcg
    15061 g
//
LOCUS       DBMDUA010000054        14719 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2231_length_14719_cov_2.810576, whole genome shotgun sequence.
ACCESSION   DBMDUA010000054 DBMDUA010000000
VERSION     DBMDUA010000054.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 14719)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14719)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14719
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2231_length_14719_cov_2.810576"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(46..633)
                     /locus_tag="ACZPCL_05940"
     CDS             complement(46..633)
                     /locus_tag="ACZPCL_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR1162051.1"
                     /translation="MIYLQLFLSFLQIGAFSFGGGYAAMPLIQNQVVQLHPWLSQSEF
                     TDLITISQMTPGPIAVNSATFVGTRIAGMPGALAATAGCVLPSCILVTLLAKIYLKYR
                     NLSLLQGVLKSLRPAVIAMIAAAGVSILVNAFWGETMASLTTTELLPNISIQAVGIFI
                     GSLILLVRFKINPIHVMLLSGVAEVVLQLIGKIFT"
     gene            complement(630..1262)
                     /locus_tag="ACZPCL_05945"
     CDS             complement(630..1262)
                     /locus_tag="ACZPCL_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR1162052.1"
                     /translation="MELNIKGASKDEILKKLFLSTLYLSAFTFGGGYVIVTLMKTKFV
                     DQYHWIDEEEMLDLVAIAQSCPGAIAVNGAIVVGYKLAGLTGVLCSVVATVIPPFVIL
                     SAISFCYAAFRSNLIVGWMLNGMQSGVGAVIMEVSFSMASGIVKEKQYLSIFIMAAAF
                     IANYFYKVSAIVLILICIALGVVLTLYREKRAQKNDRNTSQANKQKGGTL"
     gene            1793..3241
                     /locus_tag="ACZPCL_05950"
     CDS             1793..3241
                     /locus_tag="ACZPCL_05950"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1162053.1"
                     /translation="MDLEAQKRAPIYEALERFRKKRVVPFDVPGHKRGRGNPELAALL
                     GEKCVGLDVNSMKPLDNLCHPVSVIREAEELAAEAFGASHAFLMVGGTTSAVQGMILS
                     VCKAGDKIILPRNVHKSAINALVLCGAVPVYVNPEVNGDLGISLGMDIRKVEKAIEEN
                     PDAVAVLVNNPTYYGICSDLRGIVKLAHEKGMKVLADEAHGTHLYFGKGLPVNAMAAG
                     ADMAAVSMHKSGGSLTQSSMLLLNKGMNQDYVRQIINLTQTTSASYLLLSSLDISRRN
                     LALRGEESFGKVIQMAEYARNEINSIGGYYAYGKDLVNGTSVYDFDVTKLSVYTLGIG
                     LAGIEVYDLLRDEYDIQIEFGDICNILAYISIGDRIQDIERLVGALADIERLYRKDKT
                     GMLTGEYISPKVECSPQKAFYAQKVSLPIEQTAGKISGEFVMCYPPGIPILAPGEVIT
                     QEIVEYILYAKEKGCSMQGTEDPKIERLMVLV"
     gene            3279..4139
                     /gene="speE"
                     /locus_tag="ACZPCL_05955"
     CDS             3279..4139
                     /gene="speE"
                     /locus_tag="ACZPCL_05955"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HIR1162054.1"
                     /translation="MELWYSEFHTENVKLSVRVDRQLFSGESEYQRIDVFESAEFGKF
                     VALDGEIVFSEKDEFIYDEMVTHVPMAVHPNVKNVLIIGGGDGGVAKELIQYPGIESI
                     DVVETDKMFVDVCREIFPEVACGLDEERVHVYYDDGLRFLRGKKGCYDLIINDSTDPF
                     GHTEGLFTKEFYGSCYNALREDGIMVYQHGSPFYDEDEAACRSMHRKVYRSFPVSRVY
                     QAHIPTCPSGYWLFGFASKKYHPLNDLQAEKWNELHIPTWYYTTNLHTGAFMLPKYVE
                     DLLEEEENNK"
     gene            4136..5395
                     /locus_tag="ACZPCL_05960"
     CDS             4136..5395
                     /locus_tag="ACZPCL_05960"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007888721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR1162055.1"
                     /translation="MSRLMVIGCGGVASVAVHKCCQNSGVFTELMIASRTKSKCDALK
                     AQLEGKTDTVITTAQVDADKVEELEALIRSYKPDAVLNVALPYQDLTIMEACLRTGVD
                     YIDTANYEAENTDDPQWRAIYEKRCKEKGFTAYFDYSWQWDLNDRFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHTIDILDCNGGDHGYPFATNFNPEINLREVSANGSYW
                     ENGHWVETEPMEIKREYDFPEVGKKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGMLSTSPIQFQGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFNGV
                     KDGKEKTIYIYNVCDHQECYKEVGSQAISYTTGVPAMIGAMMVVTGAWKKDGVFNVEE
                     FDPDPFMDALNKWGLPWQVCEDPQIVE"
     gene            5409..6548
                     /gene="nspC"
                     /locus_tag="ACZPCL_05965"
     CDS             5409..6548
                     /gene="nspC"
                     /locus_tag="ACZPCL_05965"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045312 - nor-spermidine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HIR1162056.1"
                     /translation="MRMDQLPTPCYVIDEKKLKENLEILGDVREKAGCKILLAQKAFS
                     CFYEYPLIGQYLDGTTASGLYEARLGKEEMGKENHVFAPAYKDADIKELGEICDHIIF
                     NSFAQLRRHKDAVSGKSLGLRINPECSTQGEHAIYDPCAPGSRLGVTKEVFDCEIAAE
                     PELFEALDGLHFHTLCEQNADDLAKTLEAVEEKFGPWLSKIKWLNMGGGHHITRDDYD
                     RELLIKCIRHIRDTYGVEVYLEPGEAIALNAGYLVTEVMDIVENGLSVLILDASAACH
                     MPDVLEMPYRPPLKDSGVSGEKEFTYRLSSCTCLAGDVIGDYSFDHKIQVGDKLYFQD
                     MAIYSMVKNNTFNGIPLPGIAVMKEDGDCEMIRTFGYEDFKGRLA"
     gene            6584..7726
                     /gene="aguA"
                     /locus_tag="ACZPCL_05970"
     CDS             6584..7726
                     /gene="aguA"
                     /locus_tag="ACZPCL_05970"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HIR1162057.1"
                     /translation="MDKINKTVQAERLKTLPASDGFHMPGEFEPHKGTIMIWPERPGS
                     WAYGARDARKAFAQIAEAIAEGEDVFMLAGPSSIESAKAAFSGKSENIHILPIETDDA
                     WARDVGPTFVKNARKEVRGISWQFNAWGGEVDGLYASWEKDDAAAEAVCDALGYPVYD
                     AGDFVLEGGSIHSDGEGTLLVTEACLLSPGRNPRLTKEEIEKKLCEYLGAKKVIWLKN
                     GIWQDETNEHVDNVCAFVKPGEVVLAWTDDKEDPQYALSMEDYQILENETDAKGRKFV
                     IHKLPIPEKPVCIQEEDLKGLAFEPGEDTREAGERLAASYVNFYIANEAVLVPQFQDK
                     HDKLACDILGKLFPERKICPIDAQAIIIGGGNIHCITQQIPSGCGK"
     gene            7862..8734
                     /gene="aguB"
                     /locus_tag="ACZPCL_05975"
     CDS             7862..8734
                     /gene="aguB"
                     /locus_tag="ACZPCL_05975"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HIR1162058.1"
                     /translation="MRNVRCAAVQMRCSRNVAENIEKAEALVRQAAAEGAQVILLPEL
                     FERQYFCQERRYDYYQFATPVMENPAVRYFQRVCASLGVVIPVSFYERDVNRLFNSVA
                     MIDADGSILGVYRKTHIPDDHFYQEKFYFTPGDTGFKVFDTAYGKVGVGICWDQWFPE
                     TARAMAVEGAEILLYPTAIGSEPILSTDSMPHWRRCMQGHSASNLVPVVAANRVGEER
                     VEPCAENAGQSSSLVFYGSSFITDGCGEIQISMGRQEEGFIAATFDLDQLMEDRLSWG
                     LFRDRRPEMYGKLL"
     gene            8876..9271
                     /locus_tag="ACZPCL_05980"
     CDS             8876..9271
                     /locus_tag="ACZPCL_05980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR1162059.1"
                     /translation="MLNESVIKVLKNSMWDLATCANGEPNVVPVAFKDVTEDGKLVVG
                     DVCLETTLNHIKANDGKIAISVYDTQSLEGYQIKGTAEYVTEGDVVDTFKAMVESMFK
                     GAATAKGALIITPAKVIVTTPGADNKKEI"
     gene            complement(9268..9975)
                     /locus_tag="ACZPCL_05985"
     CDS             complement(9268..9975)
                     /locus_tag="ACZPCL_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1162060.1"
                     /translation="MSQISTEIGKQIRTFRKKRKMTLEALAAVICKSKSTVSKYENGE
                     IPVDIETLYEIASALQIHVEQLLYCPPRHANLPDSKNSPAFFAGCSQFYAYVYDGRNK
                     QLIRCVFDVLSETDTSSYKIMMYMNFKDFENYKLCENTYWGYIEHFDALTNIQLTNQD
                     SPVEKASAQILASYLDTDTKWGLFNGFSSRPMMPIATKMLFSKTKLPEDEALLQGLKV
                     SREDIRLLKLYNMFTVL"
     gene            10294..11478
                     /locus_tag="ACZPCL_05990"
     CDS             10294..11478
                     /locus_tag="ACZPCL_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1162061.1"
                     /translation="MESSKIKELAQDQQDYVVAMRREFHRSPELSGKEFHTRERLIEE
                     IEKMGVPYEKLPGTGLIAIIEGGKPGKNKILRADIDALPLQEETENLKQPKVCVSEIP
                     GRCHACGHDAHMAMLLGTMRILVQNKEELPGTVYCCFEEGEETNCGIDTMIEALEKYP
                     IDECFAFHVYNELDAGKVNIVPGPRMAGTVGIGFHLKGKSGHGSRPDQAINPIIPAAH
                     IITQLNSAFMNQLNVEETVTLGLGMVKAGEATNIIPDDAYVGGTARFFNKEEGEKALE
                     IIERIARNTADSYRCGIEFEKRNNISPYPVVNDEKTALKIQKAVGEICGEEVLGDCDK
                     WFASECYSAYQNKYPGVLGFLGIRNEAYGSGAAHHNGKFDIDESGFWLGVCAEAAFAF
                     EK"
     gene            11530..12939
                     /locus_tag="ACZPCL_05995"
     CDS             11530..12939
                     /locus_tag="ACZPCL_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HIR1162062.1"
                     /translation="MGKKEKKPFKMPHLLWIMFGIIVLACLATYLIPAGEFAVNEAGQ
                     ITGTDFHFLGHQTPVSPWGALMRVKSGMVGAATIAFVVMISGANINVVLETGVMDDLM
                     NWGVYKLKDKGTGILVSMMMILMAYLGGFGGTDALIAVVPVGVMFSKKLKLDPICALA
                     VTTFAALVGFGTGPAQQATTQMLMGVTPYSGFFTRLVIMNFFLLVAIIMTMQYIKKIR
                     KNPAASIMYQDGWRPDAIVNGSEEELLKHVEMNWRKVAVIVLFVLQYLIIAIYPLMGG
                     DSNLTFDFMLAVMIITMIVVGIIGGMSAEKLADAFAKGLGSMAFVAFVIGLAKVISLV
                     LADGNVIHTIVYVLTKPLMSLPKSVSSVGLTLIVSVINPLIPSATSKAAILVPIMKPV
                     AEALGLHLNLAVVAYQMGDSFTNLLSPLLGWMIGSCIMAEVPFPRWFKWVFPKVITLI
                     LLACVIVFLLTVTGWTGVI"
     gene            13314..14534
                     /locus_tag="ACZPCL_06000"
     CDS             13314..14534
                     /locus_tag="ACZPCL_06000"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR1162063.1"
                     /translation="MYNFDEIIDRRHTNAMNTDGFRDYIFHADETMKFPYKDEEFIRM
                     WVADMEFATPDVVIDGMRERLDKRIFGYTRVFEKSYYEAFSAWCQRRYGWTFDRKELV
                     MSNGIIPALYEMVEYICKPDEKVLFLTPSYAYFKYAADFSKRGYVCSDLHDEDGYYTI
                     DFDDLAKKAADEKTTLFILCNPHNPSGRIWTEEELKKMAEIIEKNNMWVISDEIHCDL
                     IRCDKKHIPMGKVMPDYKRLITCMAPSKTFNLAGMMISNVIIRDEGLKEIWLGRHYNF
                     DNPLSIAAAQAAYEKGEPWLEELRAYLDENFRFTGEYLKEHLPKAKYRVSEATYLAWV
                     DLNAYFEPDEHLPLFFAYKAGVLLEGGNMFVQNSDGFIRLNLACPKATLAEGLRRICE
                     AVNTKHTEKYLGEK"
BASE COUNT         4408 a         2841 c         3685 g         3785 t
ORIGIN      
        1 cttatatcat accacaaagt gagagaaaaa tatgtaataa aattctcaag taaaaatttt
       61 tcctatcagc tgcagcacca cttccgccac tcctgacaaa agcattacat gaatagggtt
      121 tattttaaag cgtaccaaca ggatcaggga gccgatgaaa atacctacgg cctggatgct
      181 gatatttggc agaagttccg tagttgtcag ggaagccata gtttcgcccc agaaagcgtt
      241 gaccaggatg ctgacaccgg cagctgcgat catggcgatc acagccggac gcagggattt
      301 aaggacgccc tggagaaggc ttaaatttct gtatttcagg taaattttag caagcaaagt
      361 gaccaggata caggatggca gaacgcagcc tgcggtggca gccaaagcac caggcattcc
      421 tgcaatgcgg gtgcctacga aggtagcgga attgaccgca atgggcccag gcgtcatctg
      481 ggaaatggtg atcaggtccg taaattctga ctgggaaagc catggatgga gctggaccac
      541 ctggttttgg atcagaggca tggcagcata gccgcctccg aagctgaagg caccgatctg
      601 aagaaaactg agaaaaagct gtaagtagat cataatgtac ctcccttctg tttgttagcc
      661 tgggaagtat tacggtcgtt tttttgggcc cttttctccc ggtacagtgt aagcaccaca
      721 cctaaagcaa tacagatcag gatcaataca atggcactga ctttataaaa gtaattagca
      781 ataaaagctg cggccataat gaaaatggac aggtactgtt tttcttttac aataccggat
      841 gccatgctaa agctgacttc cataataact gctcctacgc ctgactgcat gccatttaac
      901 atccacccaa cgatcaggtt ggaacggaat gccgcataac agaatgagat agcagaaaga
      961 atgacaaaag gcggtattac tgtagcaacc acagagcata agactcctgt caggccagcc
     1021 agcttgtaac ctacaacgat agccccgttt acagcaatgg ctccgggaca tgactgggcg
     1081 atagccacca gatctagcat ttcttcctca tcgatccagt gatactggtc tacaaatttt
     1141 gttttcatca aagtaacgat cacgtatccg ccgccaaagg taaaggcact gagatacaat
     1201 gttgagagaa agagcttttt aaggatctca tctttactgg ctccttttat atttaattcc
     1261 atattttcct cctgtgtaac agactactat acgtccagat cgtggacgta caacaacatt
     1321 ctttcttact ataagccatg aaaatccaga tgtaaatgta aaaatgtatg atgaatgaga
     1381 aaatgataag gtgatcataa aaattttctt gacagcttgt taagactatg ttaatttaaa
     1441 aagtgtgacc actcaatata ttgagttaag cccagacgga caggcgggtc agaagccgaa
     1501 agtgggaaac cacattattg attgggttag gagcccaaat tttataagtc gatacgcagt
     1561 atgcgtaatt ttatcagtgc aaaaaggcac gtcacttgtg aaacggtcaa taagagcttc
     1621 tggcaaaaca gagcagagaa ctggggagta gggtcacttt ttttctgtct gttaaaagga
     1681 cagagacatt taaagacaaa acacatacag caggtgatgg aatatgccgt cacctgtttt
     1741 ttttatgttt ttttatggat aaaagggacg gcgagtatga ggaggcggaa aaatggacct
     1801 ggaagcacaa aagcgtgcgc ctatttatga ggcgttagag cgttttagaa agaaaagggt
     1861 agtgcccttt gacgtaccgg gacacaaaag aggacgggga aacccagagc tggcagctct
     1921 tttaggggaa aaatgtgttg gattagatgt aaactccatg aaacctttgg ataacctgtg
     1981 ccatccggta tctgtgatcc gggaggcaga ggaacttgca gcagaggcct ttggcgccag
     2041 ccatgcattt cttatggtag ggggaaccac ttctgctgtt caggggatga tcctttcagt
     2101 ctgtaaagca ggagataaga tcattctgcc aagaaatgta cataaaagtg ccatcaatgc
     2161 cctggtactt tgcggggcag ttccggttta tgtaaatccg gaagtaaacg gagatctggg
     2221 catctctctt ggaatggata tccggaaggt ggaaaaagcc attgaggaaa atccggatgc
     2281 agtggcagtc ctggtaaata atcccactta ctacgggatc tgttctgacc tgcgtgggat
     2341 cgtaaaactg gcccacgaaa aaggcatgaa ggtgctggca gacgaagccc atgggactca
     2401 tttgtacttt ggaaaaggac ttcctgtgaa tgcaatggca gcaggtgcag atatggcagc
     2461 tgtttccatg cacaagtcag gaggaagcct gacacaaagc tccatgctcc ttttaaacaa
     2521 gggcatgaac caggactatg tgcgtcagat cattaacctg acacagacca ccagtgcatc
     2581 ctatcttctc ctttccagtc tggatatttc cagaagaaat ctggccctgc gtggggaaga
     2641 gtcctttgga aaagtgatcc agatggcaga atatgccaga aatgagatca attccattgg
     2701 cggctattat gcatatggaa aagatctggt caatggtacc agtgtctatg attttgatgt
     2761 gaccaagctt tctgtgtata cactggggat cggactggct ggcatcgagg tttatgacct
     2821 gttaagggat gagtatgata tccagatcga atttggagat atctgcaaca ttctggcata
     2881 catttccatt ggtgaccgca ttcaggatat tgagcgcctt gtgggagctt tggcagatat
     2941 tgagcgcctt tacaggaaag ataagacggg catgctcacc ggggaataca tttcaccaaa
     3001 agtagaatgt tccccacaga aagcttttta tgcccagaaa gtttcactcc ctattgaaca
     3061 gacggcagga aagatcagcg gtgaatttgt tatgtgttat ccgccgggta tcccgatcct
     3121 ggcgccaggg gaagtgatca cccaggagat cgtggagtac attctttatg caaaggaaaa
     3181 aggatgttcc atgcagggaa cagaagaccc gaagatcgaa cggctgatgg ttttagtata
     3241 aattgctccc gctgtgggag aacatcagga ggaagaaaat ggaactttgg tattcggagt
     3301 ttcatacgga aaatgtaaag ctttctgtcc gtgtggaccg tcagctgttt tccggtgaaa
     3361 gtgagtatca gcggatcgat gtatttgagt ctgctgagtt tggaaaattt gtagccttgg
     3421 acggggagat cgtcttctcc gaaaaagatg aatttatcta tgatgaaatg gtgacccatg
     3481 tgcctatggc tgtccaccct aatgtgaaaa atgtgctgat cattggcggc ggggacggcg
     3541 gtgtggcaaa ggagttgatc cagtatccgg gaattgagtc cattgacgta gtagaaacgg
     3601 acaaaatgtt tgtagatgtg tgcagggaga tctttccgga ggtagcctgc ggattagacg
     3661 aagagcgggt ccatgtatat tatgacgacg gactgcgttt ccttcgtgga aagaaaggct
     3721 gttatgacct gatcatcaat gactctacag acccatttgg acatacagaa ggtctgttta
     3781 caaaggagtt ttacggcagc tgctacaatg cgctgcggga agacgggatc atggtatatc
     3841 agcatggaag ccccttttat gatgaagatg aagctgcctg ccgtagcatg cacaggaaag
     3901 tataccgttc tttccctgta agccgtgtgt atcaggccca tattccaacc tgtccgtccg
     3961 gctactggct gttcggcttt gcgtcaaaga agtatcatcc gttaaatgat cttcaggcgg
     4021 aaaagtggaa tgaacttcat atacctacat ggtactatac aaccaatctc cataccggcg
     4081 cgttcatgct gccaaaatat gtggaggacc tgttagaaga ggaggaaaat aacaaatgag
     4141 cagattaatg gtgatcggat gtggaggcgt agcttccgta gcagtacata agtgctgcca
     4201 gaacagcggt gtatttacag aacttatgat cgcaagccgc acaaaatcaa agtgtgatgc
     4261 cttaaaggca cagttagaag gaaaaacaga tacggtgatc acaaccgccc aggtggatgc
     4321 agataaggta gaagaacttg aggcactgat ccgctcctat aagccggatg cagtattaaa
     4381 tgtggctctt ccttatcagg accttactat tatggaagca tgccttcgta caggtgtaga
     4441 ctacattgat acagccaatt atgaggctga aaatacagat gatccacagt ggagagcaat
     4501 ttacgaaaaa cgctgcaagg aaaaaggatt tactgcatac tttgattatt cctggcagtg
     4561 ggatctgaat gaccgtttta aagaggctgg tcttaccgcc ctgttaggaa gcggttttga
     4621 tccgggtgtt accagcgtat tttctgctta tgcattaaag cattattttg acgagatcca
     4681 taccattgat attttagact gcaacggcgg agatcatggc tatccatttg ctaccaactt
     4741 taacccggag atcaatttac gcgaagtatc tgcaaacggc tcctactggg agaatggtca
     4801 ctgggtagaa acagagccaa tggagatcaa gagagaatat gatttccctg aagtaggaaa
     4861 gaaagatatg tatcttcttc atcacgaaga gatcgagtct cttgcaaaaa atatcccggg
     4921 agttaagcgg atccgcttct ttatgacctt tggacagagc tacttaaccc atatgaaatg
     4981 cctggaaaat gtaggaatgc tttccacttc cccgatccag ttccagggac aggagatcgt
     5041 accgatccag tttttaaagg cactgcttcc tgatcctgct tccttaggtc caagaactgt
     5101 aggaaagact aatatcggct gtatctttaa tggtgtaaaa gacggaaaag aaaagaccat
     5161 ttacatttac aatgtatgcg accaccagga gtgctacaaa gaggttggtt cccaggcaat
     5221 ttcctataca accggtgttc cggctatgat cggcgctatg atggtagtta ccggtgcatg
     5281 gaaaaaagac ggcgtgttta acgtagagga atttgatccg gatccattta tggacgcctt
     5341 aaataagtgg ggacttccat ggcaggtatg cgaagatcct cagatcgtag aatagggaga
     5401 acttgaaaat gagaatggat cagcttccca ctccctgtta tgtgatcgat gagaagaaat
     5461 tgaaagaaaa tctggagatc ttgggggatg tccgggaaaa agcaggctgt aagatcctgc
     5521 tggcccagaa agccttttcc tgcttttatg aatatcctct gatcgggcag tatttagacg
     5581 gcaccacagc cagcggcctg tatgaggcca gacttggaaa agaagaaatg gggaaagaaa
     5641 accatgtgtt tgccccggct tataaggatg cagatataaa agaactggga gagatctgtg
     5701 accacattat ctttaattcc tttgcccagc ttcgccgcca taaagacgca gtctcaggaa
     5761 aaagcctggg actgcgcatc aacccggagt gttccactca gggggagcat gccatttatg
     5821 atccctgtgc tcctggttca agacttggcg ttacaaaaga ggtatttgac tgtgagattg
     5881 cagcagagcc ggagcttttt gaggctctgg acggcctgca ttttcatacg ctttgcgagc
     5941 agaatgcaga tgatctggca aaaacactgg aagcggtgga agaaaagttc ggcccatggc
     6001 tttctaagat aaaatggctg aatatgggcg gcggtcatca tattacaaga gatgattatg
     6061 acagagagct gcttataaaa tgcatccgtc atataagaga cacttatgga gtggaagtgt
     6121 atttggagcc gggagaagct attgccttaa atgccggtta tctggtgaca gaggtaatgg
     6181 atattgtgga aaatggactt tctgtgctga tccttgacgc ttctgctgcc tgccatatgc
     6241 cggatgtgct ggaaatgcca tacagaccgc cattaaagga cagcggagtt tcaggagaaa
     6301 aggagtttac ctaccgcctt tcctcctgca cctgtctggc aggagatgtg atcggggact
     6361 attcctttga tcacaagatc caggtgggag ataagctgta ttttcaggat atggctattt
     6421 attccatggt gaaaaataat acatttaacg gtattcccct tcctgggatc gctgtgatga
     6481 aagaggacgg ggactgcgag atgatccgca cttttggata tgaagacttt aaggggagac
     6541 tggcataatg gtgatggtgg aaaagacgga tatgaccggt gaaatggata agataaataa
     6601 aacggtgcag gcggaacggc taaaaacatt gccggcatca gatggatttc acatgccggg
     6661 agagtttgaa cctcacaaag gcaccattat gatctggccg gagcgccccg gttcctgggc
     6721 ttatggagcc agagatgcca gaaaagcttt tgcacagatc gcagaggcca tagcagaagg
     6781 agaagatgtt ttcatgcttg ctggtccttc ttccatcgag tctgcgaaag cggctttttc
     6841 tggaaagtca gaaaacatcc acattcttcc tatagaaaca gacgatgcct gggccagaga
     6901 tgtaggacct acctttgtaa aaaatgcccg gaaggaagtc cggggcatca gctggcagtt
     6961 caatgcctgg ggcggtgagg tagacggcct ttatgcaagc tgggaaaagg atgatgctgc
     7021 ggcagaggcg gtctgcgatg ctcttggtta tcctgtatat gatgcaggag attttgtcct
     7081 ggaaggcggt tccattcaca gtgacggaga aggaacgctg ttagtaacag aagcatgtct
     7141 tttaagtcct ggaagaaacc cacgccttac aaaagaagag attgaaaaga agctttgtga
     7201 atatctgggt gcaaaaaagg tgatctggct gaaaaacggc atctggcagg acgagaccaa
     7261 tgagcatgtg gacaatgtgt gcgcctttgt aaagccggga gaggtagttt tagcctggac
     7321 cgatgataaa gaagatccac agtatgccct ttccatggaa gattaccaga ttttggaaaa
     7381 tgagacagat gcaaagggca gaaagtttgt gatccataaa ctgccaattc ctgaaaagcc
     7441 ggtatgcatc caggaagaag acttaaaagg tcttgctttt gagccgggag aagataccag
     7501 agaagcagga gaacggctgg cagccagtta tgtaaatttc tatattgcaa atgaagcagt
     7561 tttagttcct cagtttcagg ataagcatga taagctggcc tgcgatattt taggaaagct
     7621 gttcccggag cggaagatct gcccgattga tgcacaggcc atcatcatag gtggcggaaa
     7681 tatccattgt attacccagc agatcccttc aggctgtgga aagtaaaatt cggcaaccag
     7741 cagtaaccgc aaaagttgcc aacgaaaata aaaatggatg aaacattata aaatgttgga
     7801 aaaacaacgg atcaaatgga tttgggaata tcgggtccag tgaaagaagg agaaacgaaa
     7861 attgagaaac gtaagatgtg ccgcagtcca gatgcgctgc agcagaaatg tagcagaaaa
     7921 catagaaaaa gcagaggctc tggtacgtca ggcagcagcg gaaggagctc aggtgatcct
     7981 tcttccagag ctgtttgaac gtcagtattt ctgccaggag cgcagatatg attattacca
     8041 gtttgcaacg cctgtaatgg aaaatccggc agtgagatat ttccagagag tctgtgcaag
     8101 ccttggagta gtgatcccgg taagctttta tgaaagagat gtaaaccggc tgtttaattc
     8161 tgttgctatg atcgatgcag acggttctat tctgggcgtt tacagaaaga cccatatccc
     8221 ggatgaccat ttctatcagg aaaaatttta tttcacaccg ggagataccg gttttaaggt
     8281 atttgacaca gcctatggaa aagtgggcgt tgggatctgc tgggaccagt ggtttccgga
     8341 gactgccaga gctatggcag tagaaggcgc agagatcctt ctgtatccaa cagctattgg
     8401 aagtgaacct attctttcca ctgacagtat gcctcactgg agaagatgta tgcagggaca
     8461 cagcgcatct aatctggtgc ctgtggttgc agcgaaccgg gtaggcgagg aacgggtaga
     8521 gccatgtgca gaaaatgcag gacagagttc ttcccttgtt ttctacggtt catcttttat
     8581 caccgacggc tgtggagaga tccagatatc catgggaaga caggaagaag gttttatagc
     8641 agctaccttt gatctggacc agctgatgga agaccgcctt tcctggggac ttttcaggga
     8701 cagacggccg gagatgtatg gaaagcttct gtaaaaagat cacagataac actatgaaaa
     8761 atagtagtga cttataaatt gactaatatc ttccagaaca gtagcagatc cgataagata
     8821 agtactgtca ataagcaatg cagacatgca aaagcaacga atggaggtat attcaatgtt
     8881 aaatgagtca gtgatcaaag ttttaaaaaa tagtatgtgg gatcttgcta cctgcgcaaa
     8941 tggagagcca aacgtagttc cagttgcatt taaggatgta acagaggacg gaaagctggt
     9001 tgtaggtgat gtttgccttg aaacaacttt aaaccacatt aaggcaaatg acggaaaaat
     9061 tgctatttct gtatatgaca cacagagcct ggaaggatat cagatcaagg gaactgctga
     9121 gtacgtaaca gagggcgatg ttgtagatac ctttaaggct atggtagaga gcatgtttaa
     9181 gggtgctgca actgcaaagg gagcgcttat catcacacct gccaaagtga tcgttaccac
     9241 acctggtgct gacaacaaga aagaaatcta aagcactgta aacatattat aaagctttaa
     9301 caaacgtata tcttcccggg agactttaag gccctgcaac agagcttcgt cttccgggag
     9361 ttttgttttt gaaaacagca ttttagtagc gatgggcatc atgggacggc tggaaaaacc
     9421 attaaacagt ccccattttg tatctgtatc cagataggaa gcaaggatct gtgcgctggc
     9481 tttttctacc gggctgtcct ggttggtcag ctggatattg gtcagggcgt caaaatgctc
     9541 aatatagccc cagtatgtat tttcacacag cttgtaattt tcaaaatctt taaaattcat
     9601 atacatcatg atcttataac ttgatgtatc agtttctgaa agcacatcaa agacgcagcg
     9661 gatcagctgc ttatttcttc cgtcatatac ataggcataa aactgggagc agcctgcgaa
     9721 aaaggcggga ctgtttttgc tgtccggcag gttggcatgg cgtggcggac agtagagcag
     9781 ctgctctacg tggatctgca aagcagaggc aatttcatat aatgtttcaa tatctaccgg
     9841 aatctccccg ttttcatact tggaaacagt agatttgctt ttgcagataa cagccgcaag
     9901 ggcttccagt gtcattttgc gttttttgcg gaaagtgcgg atctgttttc cgatttctgt
     9961 actgatctgg gacatagatt aagccactcc ttttatagat taaagtaagg atccaactgt
    10021 atttttaatg actttatgat agcaaaaagt ttcgtataaa tcaacaaaac cgcgattttt
    10081 ttctgcagtt tttcaggcgg ggccaaaagc aggtgttgga aagaaaaaaa tccagtttca
    10141 ggaaacaagt tttagatttt tccattaaga ggaaacaaat gcctttttgc ttcagcgtat
    10201 tttttttagt acaatgcaga tatgagatgc atccaggaaa tcagttaaat gacaggtttg
    10261 cgcagcctga caaacaaaag caggaggaaa tgaatggaat ccagtaagat aaaagaactg
    10321 gcacaggacc agcaggacta cgtagtggct atgaggcggg aatttcaccg ttcaccggag
    10381 ctttcaggaa aagaattcca cacaagagaa cggttgatag aagaaatcga aaaaatgggt
    10441 gttccctatg aaaagcttcc aggaacaggc cttattgcga tcattgaagg cggaaagcct
    10501 gggaagaata aaatactgcg tgcagatata gatgctctgc cattacagga agaaactgaa
    10561 aatttaaagc agcctaaggt ctgcgtttct gaaattccag gcagatgtca tgcctgcggc
    10621 catgatgccc atatggcaat gctccttgga acaatgagaa tactggtgca gaacaaagaa
    10681 gaacttccag gaactgtata ttgttgtttt gaagaaggag aggaaacaaa ctgcggaatt
    10741 gataccatga tagaggcact ggaaaaatat cctattgatg agtgctttgc atttcacgta
    10801 tataacgaac tggatgcagg caaggtcaac attgttccgg gaccaagaat ggcaggaaca
    10861 gtaggaattg gtttccacct aaaaggaaag agcggacatg gctccagacc ggatcaggcg
    10921 atcaacccga tcattccggc agcccatatt atcacacagc tgaattctgc ttttatgaac
    10981 cagttaaatg tagaagaaac agttacactt ggtcttggaa tggtaaaagc tggagaagct
    11041 accaacatta tcccggatga tgcttatgta ggaggaactg cccgtttctt caataaagaa
    11101 gaaggagaaa aggcattaga gatcattgag cggatcgcaa gaaatacagc agattcctac
    11161 agatgcggca ttgaatttga aaagagaaat aatatcagcc catatcctgt tgtaaatgat
    11221 gagaaaacag ctcttaagat ccagaaagca gtaggtgaga tctgtggaga agaagtatta
    11281 ggagactgtg ataagtggtt tgcttctgag tgctacagtg catatcagaa caaatatcca
    11341 ggcgtgctgg gatttttagg gatccgcaat gaagcttatg gcagcggtgc agcacaccat
    11401 aatggaaaat tcgatataga tgaatcagga ttctggctgg gagtgtgtgc agaagcagcc
    11461 tttgcatttg aaaaataaaa acagggaatg gtttgaaaaa tacgtaagag tataaagagg
    11521 aaaaactaaa tgggaaaaaa agaaaagaaa ccgtttaaaa tgccgcatct tttgtggatt
    11581 atgtttggga tcatagtatt ggcctgtctg gcaacctatc tgatcccggc cggagagttt
    11641 gcagtaaatg aagcaggaca gatcacaggt actgatttcc acttcctggg acatcagaca
    11701 ccggtcagtc cgtggggcgc actgatgcgt gtgaagtcag gcatggtagg agcagcaacc
    11761 atagcgtttg ttgttatgat atcaggagct aatatcaacg ttgtactgga aacaggcgtt
    11821 atggacgatc tgatgaattg gggtgtttat aagttaaagg ataaaggaac aggtatcctg
    11881 gtatccatga tgatgatcct tatggcttat ttaggtggat ttggtggaac agatgccctg
    11941 atcgctgtag taccggttgg tgttatgttc tctaaaaagc tgaagctgga tccaatctgt
    12001 gcccttgcag taaccacctt tgcagcatta gtgggctttg gtacaggtcc tgcgcagcag
    12061 gcaactacac agatgcttat gggcgtcact ccttattctg gtttctttac caggctggtc
    12121 atcatgaatt tctttttact tgttgcgatc attatgacta tgcagtatat caaaaagatc
    12181 cgcaaaaatc ctgctgcatc tataatgtat caggatggct ggagaccgga tgctattgtg
    12241 aacggttcag aggaagaact gttaaagcat gtggaaatga actggagaaa agtagcagtt
    12301 attgttcttt ttgtacttca gtatctgatc attgccattt atcctcttat gggcggagac
    12361 agtaacctga cctttgactt tatgcttgca gttatgatca ttactatgat cgtagtaggc
    12421 attatcggcg gcatgtctgc tgaaaagctg gctgatgctt ttgcaaaagg ccttggctct
    12481 atggcttttg tagcttttgt tatcggactt gcaaaggtga tctctctggt gctggcagat
    12541 ggaaatgtga tccataccat tgtgtatgtt cttaccaagc cgcttatgag tcttccaaaa
    12601 tccgtatcca gtgtaggact tacactgatc gtttctgtta tcaacccgct gatcccgtct
    12661 gctacttcaa aggctgcgat cctggttcct attatgaagc cggtagcaga agcactgggc
    12721 ttacatctga accttgcagt tgtagcttat cagatgggcg acagctttac caacctgtta
    12781 tccccgcttt taggctggat gatcgggtcc tgcattatgg cggaagtgcc atttccaaga
    12841 tggtttaaat gggtgttccc aaaggttatc acccttattt tactggcatg tgtgatcgtt
    12901 ttcctgctga ctgttaccgg atggaccggc gtaatatagt tatacagtga tataaatgag
    12961 agatgagcct gcattgaaaa ggctttatct ctcatttttt gtttgtgtat gtggtgaagg
    13021 gatctgtgac atattcaccg gtcaaaagcc ctgaaaagtt tcgtataaat caataaatca
    13081 acgatttctt ttctggttta cctgcttttt ctaaagtctc tgcacagaaa aaagaaaaat
    13141 ctattttcag gaaacttaat ttgtaaaatt ccactgtaga gaaacttaaa gcactttgat
    13201 ttcgggtact tattttagta caatccaggc atgagatgca tccagtacct gaaataaaaa
    13261 taacagacca ggccggcgca agcctggaaa atcagagaaa cggggtaaaa aatatgtaca
    13321 attttgatga gatcattgac agacggcata caaatgccat gaacacagac ggtttccgtg
    13381 attatatttt ccatgcagat gagactatga aattcccata caaggatgaa gaatttatcc
    13441 gtatgtgggt agcagatatg gaatttgcca caccggatgt tgttattgac ggaatgagag
    13501 agcgtttaga caagcgtatc ttcggctata ccagagtatt tgaaaagagc tattacgaag
    13561 cattttccgc atggtgccag agaagatatg gctggacctt tgacagaaaa gaactggtaa
    13621 tgtcaaatgg tatcattcca gcgttatatg aaatggtgga atacatctgc aaaccagatg
    13681 aaaaggtatt attcttaact ccttcttatg catatttcaa gtatgcagca gactttagca
    13741 agagaggcta tgtatgcagt gaccttcatg atgaagacgg atattatacc attgattttg
    13801 atgatcttgc caagaaggca gcagacgaga agactacact gtttatcctg tgcaatcctc
    13861 ataacccaag tggacgtatc tggactgaag aagaattaaa gaagatggca gagatcattg
    13921 aaaagaacaa tatgtgggtc atctctgatg agatccactg tgatttgatc cgctgcgata
    13981 aaaagcatat cccaatgggc aaggtaatgc cggattacaa gcgcctgatc acctgcatgg
    14041 caccaagcaa gacctttaac ctggcaggta tgatgatctc caacgtgatc atccgtgatg
    14101 aaggcttaaa agaaatctgg ttaggccgtc attataactt tgacaacccg ttaagtattg
    14161 cagcagcaca ggcagcttat gaaaagggcg agccatggct ggaagaatta agggcatatc
    14221 tggatgagaa cttccgcttc accggagaat acttaaagga gcatctgcca aaggcaaaat
    14281 acagagtatc tgaagcaacc tatcttgcgt gggtagactt aaatgcctac tttgagccag
    14341 atgagcacct gcctctgttc ttcgcataca aggccggagt ccttctggaa ggaggcaaca
    14401 tgttcgtaca gaactctgat ggattcatcc gtctgaacct ggcttgcccg aaggcaactt
    14461 tggcagaagg tttaagaaga atctgcgaag cagttaatac aaaacataca gaaaagtatc
    14521 tgggagagaa ataaaagctt atttcaggaa aactttcaga aacaggagaa gaaaaaggaa
    14581 aatggaaaag cgggaatttt tagaaaaata tatagctgaa tacaatgtaa aagatgtaga
    14641 agtagtactt acagggtgtt ttgggttctg tgagaaagga ccaatagtta aaatagtacc
    14701 tgaaaatacg ttctatatc
//
LOCUS       DBMDUA010000055        14680 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2238_length_14680_cov_2.452569, whole genome shotgun sequence.
ACCESSION   DBMDUA010000055 DBMDUA010000000
VERSION     DBMDUA010000055.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 14680)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14680)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14680
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2238_length_14680_cov_2.452569"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="ACZPCL_06005"
     CDS             complement(<1..290)
                     /locus_tag="ACZPCL_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR1162064.1"
                     /translation="MSFSGLFQTQGMLFLLLLIGYIFGKKGLADGTGKNLLTDLVIYV
                     TLPGSIIKSFQIEFNHQILIACMTIAIAGIVIQTGSSVLGMFLYNHYGDSKK"
     gene            complement(421..1716)
                     /gene="eno"
                     /locus_tag="ACZPCL_06010"
     CDS             complement(421..1716)
                     /gene="eno"
                     /locus_tag="ACZPCL_06010"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005346694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR1162065.1"
                     /translation="MKHLEIEKVIGREIIDSRGNPTVEAEVYLTDGTVGRGTAPSGAS
                     TGEFEALELRDGDKSRFGGKGVLKAVENVNTVISQVLKGMDPSDVYAVDRAMIQADGT
                     KDKSNLGANAILAVSIASARAAANALQIPLYRFLGGANGNRLPLPMMNILNGGAHAAN
                     TVDVQEFMIMPAGAPSFKEGLRWCAEVFHALAALLKERKLATSVGDEGGFAPDLGSDE
                     EAIQCILEAVKRAGYEPGKDFVLAMDAASSEWKGSVKGEYILPKCGKKFTSKELIAHW
                     KDLCEKYPIYSIEDGLDEEDWEGWQLMTKELGDKVQLVGDDLFVTNTERLSKGISLGC
                     GNSILIKLNQIGSVSETLEAIKMAHKAGYTAIASHRSGETEDTTIADLAVALNTCQIK
                     TGAPSRSERVAKYNQLLRIEEELGAAAQFPGFGAFNTKR"
     gene            complement(2064..3392)
                     /locus_tag="ACZPCL_06015"
     CDS             complement(2064..3392)
                     /locus_tag="ACZPCL_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HIR1162066.1"
                     /translation="MELTRENYNETAKELLTFLQNSPSCFHAVKNVVDMLTAAGFTEV
                     KEEESWKLEAGGRYFVTRNQSSVIAFKVPGKDFKGFQIIASHSDSPTFKIKENPEMEA
                     AHCIKLNVEKYGGMICAPWFDRPLSIAGRLVVSEGSRLVSKLVNVDRDLVMIPNLAIH
                     MNRQVNDGYAYNAQKDMLPVFGSLEAKGTFMSLIAEAAGVKEEDILGHDLFLYNRQPG
                     TIWGADETFLSCGRLDDLQCAFSSVKAVIEGENSSSISVAAIFDNEEVGSGTKQGADS
                     TFLKDVLERINDSLGRTREQYLMAVASSFMISADNAHAVHPNHADVADPTNRPAINEG
                     IVIKYNANQKYTTDAVSAAVFKALCKKVQVPVQTFTNRSDIAGGSTLGNISNAHVSLN
                     TVDIGLPQFAMHSPYETAGVKDTCYLIKAATEFFNSCIVAEGNGNYSLGE"
     gene            complement(3487..4917)
                     /locus_tag="ACZPCL_06020"
     CDS             complement(3487..4917)
                     /locus_tag="ACZPCL_06020"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR1162067.1"
                     /translation="MKKITAKALAQAAGGVLAHEGNEPVAEHISLDSRKMAGNDLFVP
                     IIGERADGHDYICMAIGNGATVVFTSRHKTEEEVQEAILTQCKGDKEKETAAKAAAWI
                     CVPDTRKALQDFGAYCRRNNPIPLVGITGSVGKTTTREMVAAALSAGFLVYKTPGNSN
                     SQVGVPITVAEIPENAEIGVIELGMSEPGEMTRIANVAQVNCAVMTNIGVAHIEQLGS
                     QENILKEKLHIQDGMSEKGTLFLNGDDPLLRDVVPEGGRKKVLYGLSEGCDYRGEDLH
                     LEEGYPVFTAVHGDEKVQLRLQVMGVHMVQNALAALAVAGEYGISMEMAAEPLCRFEG
                     FKGRQQIVHLNGVTVIDDSYNASPVSMKAGIEVLGSLPCEGKRIAVLADMKELGPDTV
                     KFHQEVGAFLKEHPADQVVLYGELAKEIGAGLKAAGGDIPLTEVKSLEELEQWLDDHV
                     AFGDCLLFKGSNSMGLSKAVAYMKEK"
     gene            complement(4920..6425)
                     /locus_tag="ACZPCL_06025"
     CDS             complement(4920..6425)
                     /locus_tag="ACZPCL_06025"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR1162068.1"
                     /translation="MIFRDWLKELDYTLLQGSVDVETEDVIYDSRKAAPGKVFVAMKG
                     IRVDGHSFIPQVLKAGVRVLVTERPVEEELKNSGLSEDELKQVTVVMVEEGRHALALL
                     SAARFDHPAKKLVMIGVTGTKGKTTTTHMIRAILLAAGKKVGMIGTTGTVIGDVVTPT
                     LNTTPESYELHQAFAQMVEAGCEYAVMEVSSQGIKMHRTDGLYFNYAIFTNISPDHIG
                     PGEHADFEEYLYYKTRLLNMCGIGLVNKDDEHAPQIIKAANCELYTYSEESKEADFVA
                     SDIRYVSQPDFVGTEFHISGKYDMEVRLGIPGLFNVVNALAAVSVCSFLGLDQARISH
                     ALENIRVNGRMEIVYTSERCTVLVDYAHNAVSMESLLSTLRDYKPKRLVCVFGCGGNR
                     SKDRRYSMGEIGGKMADLSIITADNSRYEKVEDIIADIRGSIEKTGGKFIEIPDRREA
                     IRYSILHAQPGDMVAVIGKGHEDYQEINGVRHHFLDREVIEETVKELGEKA"
     gene            complement(6487..8154)
                     /locus_tag="ACZPCL_06030"
     CDS             complement(6487..8154)
                     /locus_tag="ACZPCL_06030"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR1162069.1"
                     /translation="MKTDIEIAQEAVMQPIKEVAASYGIGEDDLELYGKYKAKLSDEL
                     WEKVKGRPDGKLVLVTAINPTPAGEGKTTITVGLGEALGKMGKNAIIALREPSLGPCF
                     GIKGGAAGGGYAQVLPMEDLNLHFTGDFHAITSANNLLAALLDNHIQQGNALGIDPRQ
                     ILWKRCLDMNDRVLRNVVVGLGAKADGMVREDHFVITVASEIMAILCLANDMEDLKDR
                     LGKIIVAYNFAGEPVTAADLHAVGSMAALLKDALKPNMIQTLEHTGALVHGGPFANIA
                     HGCNSVRATKTALKLCDIVVTEAGFGADLGAEKFMDIKCRKAGIHPDAVVLVATVRAL
                     KYNGGVPKDQLSEENLDALKRGIVNLEKHIENIQKFGVPVVVTLNSFITDTEAEYAYI
                     KRFCEDRGCEFALAEVWAKGGEGGMALAEKVLNTLENKRGDFKPLYTDEMPLKDKIGT
                     IASEIYGADGVSYAPAASKALKRIEEMGFGNLPVCMAKTQYSLSDDQTKLGRPAGFTI
                     NVRDAYVCAGAGFVVVLTGSIMTMPGLPKKPAAEGIDVVNGKITGLF"
     gene            complement(8195..11272)
                     /locus_tag="ACZPCL_06035"
     CDS             complement(8195..11272)
                     /locus_tag="ACZPCL_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE family DNA translocase"
                     /protein_id="HIR1162070.1"
                     /translation="MRAEVIIICSFAVAILLFLSNFGLCGIVGQILKGIQLGLFGILG
                     YLLPILIFVGTCFHLSNQGNIHAAIKLGAVIGALITLCGLFQLLFGRMPQGGSVMQIY
                     ADASNGGAGGGLVGGALAMALMFFVGRAGAYLVFLVILILCMVCITGKSFVSAVKQGS
                     GRAYQYAREDMDRRKEIHAQRAEERKLLREEQRVRGVNLNATRLTAVDEGRRPVPEDA
                     EYDEEAFEREFGTKQQAAEPEMDEIPEEKPAATGKNRVVYEEEEEIPSPADEFRGKIQ
                     MPSGYEKNKDEKDEAEALADELEQQYETIHVPKDRRTLEEMEIPAPDVIPAGRPTAWE
                     QKVSAVSEHAIKEEEEPLPKEIQRVPVEEPLWDEDLPKETVSLADLEEEERAAEKATE
                     TPVYNSEKTEYEEKSHINDEEKPQTKRNFEQSEDWEEITEEPEESPWDPKLDESVSPD
                     PLAAASGSIYVPEEKKQIVTASGKVIEAETDLLKKKLESTRAAEAAASVPKADPFMAP
                     RSSNEKVRITPPSEEESSEAVAKEIAKQEVEKEYIFPPTDLLKKPVRNNNAFSENEYK
                     ATAIKLQQTLHNFGVGVTVTNISCGPSVTRYELLPEQGVKVSKIVGLTDDIKLSLAAA
                     DIRIEAPIPGKSAVGIEVPNKENNIVYLRELLDSDSFKNHKSRLAFAVGKDIGGQVVV
                     TDIAKMPHLLIAGATGSGKSVCINTLIMSIIFKSDPKDVKMIMVDPKVVELSVYNGIP
                     HLLIPVVTDPKKASGALNWAVAEMTDRYKKFAECNVRDLKGYNEKVDKLTDIPDDKKP
                     KKLPQIVIIIDELADLMMVAPGEVEDSICRLAQLARAAGIHLVIATQRPSVNVITGLI
                     KANVPSRIAFAVSSGVDSRTIIDMNGAEKLLGKGDMLFYPAGFPKPQRVQGAFVSDEE
                     VGRVVEFLTEQGMTAQYNEDVISRVAAPSSDGGSSSAGRDEYFAQAGKFIIEKDKASI
                     GMLQRVFKIGFNRAARIMDQLAEAGVVGEEEGTKPRKVLMSPEQFEELLEQGR"
     gene            complement(11569..12504)
                     /locus_tag="ACZPCL_06040"
     CDS             complement(11569..12504)
                     /locus_tag="ACZPCL_06040"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR1162071.1"
                     /translation="MKIFDLHCDTIEALKKHNETFDNSTTQFTMKGQEKLEKAVQFLA
                     VWLPDHLRGQEAVDFTNGYYEYMTEAVGKVADRAALVEDMSDLDRILKEGKWAFIRSI
                     ESGAALGGKLENVDHFAKLNFKMMTLTWNGINELGSGFQSEMGLTDFGKAAVRRMEEV
                     GMIVDCSHLNDAGFEDLLNCSTKPFVASHSNLRSLCSHSRNLRDDQFKEIVARGGLCG
                     INIYSRFLTEPDDLGAGQKEQLLRLIDRMLELGGEDVIAWGMDLDGGVTCDPSIGTPY
                     GAAAYGEYLVEHGISREIVDKLSFDNAYRFFTKAL"
     gene            complement(12568..13338)
                     /locus_tag="ACZPCL_06045"
     CDS             complement(12568..13338)
                     /locus_tag="ACZPCL_06045"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007037538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0051117 - ATPase binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP family protease"
                     /protein_id="HIR1162072.1"
                     /translation="MGKNQKDKKEGKQENKAAAPSQERKLQEKEIEEKEDTHLKNWGQ
                     MTLDGNEKKRKIHLLSIIGEVEGHENASGGSKTTKYDHVLPALAQIEDDATVEGLLVL
                     LNTSGGDVDAGLAIAEMIASMSIPVVSLVLGGSHSIGVPLAVASDYSFIVPSGTMIVH
                     PVRMSGMVIGTAQTYEYFAMIQDRILNFVSSHADIAYDQLKALMHNTKMLARDLGTVL
                     VGQEAVDAGLINEVGGIREALKKLYEMIDESCAKKQGF"
     gene            complement(13427..14191)
                     /locus_tag="ACZPCL_06050"
     CDS             complement(13427..14191)
                     /locus_tag="ACZPCL_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024950.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1162073.1"
                     /translation="MKNYFDVAGKYAVVTGASSGLGRQFALCLAEQGCNVAIAARRVE
                     RLEEVKKEVEAYGVKCLVLPCDVTDTEQIRNSVDKVVKEFGRIDILINNAAASFATPV
                     VETTDEQWYNVMKTNVDGVFFFAREVGKVMIAQNYGKIINIGSFHCQVTMNGVPRSGY
                     STAKGAVLMMSKALAAEWAKNNITVNVLGPGYFASEMSAKVANEVYEASIRNNCPMGR
                     RGNPGELNGAMLFLASDASSYVTGQLLLVDGGWTIV"
     gene            complement(14188..14448)
                     /locus_tag="ACZPCL_06055"
     CDS             complement(14188..14448)
                     /locus_tag="ACZPCL_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024591.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR1162074.1"
                     /translation="MKQDVKQVCVLGMPDPVWGEQVTACIVLKEGSKLTEDDIVAWCK
                     QHIASYKKPRKVIFFSQLPENANGKVSRILLKDQIQKGEIKS"
BASE COUNT         3598 a         3820 c         2851 g         4411 t
ORIGIN      
        1 ttctttttgc tgtctccata gtgattataa agaaacattc caagaacaga gcttcctgtc
       61 tgtattacga tccccgcaat agcaatggtc atacaggcaa tcaggatctg gtggttaaac
      121 tcgatctgaa aggatttgat aatacttccg ggaagagtaa catagatcac cagatcagtc
      181 agcagatttt tgcctgtgcc atctgccagt cccttcttcc cgaaaatata cccgatgagc
      241 aaaagcagaa acagcattcc ctgtgtctga aataagccgg aaaaactcat aagaactacc
      301 tcaactttta tcttttgtct gctggctttc agccagtccg gcttttattc tagcacactc
      361 tttatctgta aaacaagtac ctatctgtat cagttcgttt atatactgtt tcggctgtat
      421 ttatcttttt gtattaaaag cgccaaagcc tgggaactgt gcagctgcac ccaattcttc
      481 ctcaatacga agcagctgat tgtacttggc aactctctcg cttctgcttg gagcaccggt
      541 cttgatctgg caggtattta acgcaacagc caggtctgca atggtggtat cctcagtttc
      601 accggaacga tgggatgcga ttgctgtata gccagcctta tgagccatct taatagcctc
      661 taaagtctcg gaaacgctgc cgatctggtt taacttgatc agaatggagt tgccgcagcc
      721 aaggctgatg cccttggaca gacgctctgt attggttaca aacaggtcat caccaactaa
      781 ctgaaccttg tcgcccagtt ccttggtcat taactgccag ccttcccagt cttcctcatc
      841 cagaccatcc tcgatggagt agatcgggta tttctcacac agatctttcc agtgtgcgat
      901 cagttctttg gaagtaaact tctttccgca cttaggaagg atgtactcgc cctttacact
      961 gcccttccac tcactggaag ctgcatccat tgccagtacg aagtcttttc ccggctcgta
     1021 acctgcacgt tttactgcct ctaagatgca ctggatggct tcttcatcac ttccaaggtc
     1081 tggtgcaaaa ccgccctcat caccaacaga agttgccagt tttctctcct ttaataaggc
     1141 agctaatgca tggaatactt ctgcacacca gcgcagtcct tctttgaaac ttggagcgcc
     1201 tgccggcatg atcataaatt cctgcacatc tacggtattt gctgcatgag cgccgccgtt
     1261 taagatgttc atcataggaa gtggaagtct gtttccgttt gctccgccaa ggaaacggta
     1321 cagcgggatc tgcagtgcat ttgctgctgc tcttgcactt gcgatagata cagctaaaat
     1381 agcgtttgcg cctaaatttg atttatcttt tgttccgtct gcctggatca tagcacggtc
     1441 tactgcataa acatcagaag gatccattcc ctttaatacc tgtgaaataa cggtatttac
     1501 attctcaact gcctttaaaa caccttttcc gccaaaacgg ctcttgtcac cgtcacgcag
     1561 ctccagtgct tcaaactcac ctgtagatgc gccgcttggt gcggttcctc tgcctaccgt
     1621 tccgtctgta agataaactt ctgcctctac agtaggatta cctcttgaat caatgatctc
     1681 tcttccgatt actttttcga tttccaaatg cttcataatt ttgtcctttc tggcaacgga
     1741 gttttccccc ggcaggtcat accgggggac ttggccatga tcatttcaca ttagttagtc
     1801 ttttctaacc gttaatattt tagcatagtt gggtggcaaa cgcaatgtgg cttataggga
     1861 agttttttca agtagaagtg aggccgcgcc ctgacgggcg cagatgggca aagccctggg
     1921 gagccgagcg agaaccaggg tagtccccaa gaggctgaag tggccatgcg catttacata
     1981 caaaaacagg gcctcctatt tatagaatgc cctgcttttt tgaatttatt ctattttacc
     2041 tttcccaaca gaggagttgt ctcttactca cccagactat aatttccatt tccctcagct
     2101 acaatacagc tgttgaaaaa ttcggttgct gccttgataa gatagcaggt atctttcacg
     2161 cctgccgtct cataaggaga atgcattgca aactgaggaa gtccaatgtc tacggtattt
     2221 aaggaaacat gggcattgga gatatttccc aatgtggaac cgcctgcaat gtcagaacgg
     2281 ttggtgaagg tctgtaccgg aacctgtact ttcttgcata atgctttgaa aacagctgcg
     2341 gatacagcat cggtggtgta cttctggttt gcgttgtatt taattacaat accttcgtta
     2401 atagccggac ggttggttgg gtctgctaca tctgcgtggt ttggatgtac tgcatgagcg
     2461 ttgtctgcag agatcataaa gctggaagca actgccataa gatactgctc tctggtacgt
     2521 cctaaactgt cgttgatccg ctctaaaaca tcctttaaga aagtagagtc tgcgccctgc
     2581 ttggtaccgc ttcctacttc ttcgttgtca aagatagctg ctacagaaat actggagctg
     2641 ttttctcctt cgatcactgc ttttacggaa gagaaggcac actgaaggtc atccagtctt
     2701 ccacaggaaa ggaaggtctc atctgcgccc cagatagttc ctggctgacg gttgtataag
     2761 aacagatcat gtcctaaaat atcctcttct tttacacctg cagcttctgc gatcagagac
     2821 atgaaggttc cctttgcttc cagactgcca aatacaggaa gcatatcctt ctgtgcattg
     2881 taagcatagc cgtcatttac ctgacggttc atatggatgg ccagatttgg gatcatcacc
     2941 agatcacggt ctacatttac cagcttggat accagacggc ttccctcaga aactaccagt
     3001 cttccggcaa tggataatgg tctgtcaaac catggtgcgc agatcatgcc gccgtatttt
     3061 tctacattta acttgatgca gtgagctgct tccatctccg gattttcctt gatcttaaag
     3121 gttggggaat cgctgtggct tgctatgatc tgaaagccct taaaatcttt tcccggtacc
     3181 ttaaatgcga tcacagaaga ctgattgcgg gtcacaaaat aacggccgcc agcctcaagt
     3241 ttccaacttt cttcctcttt cacctctgta aaaccggctg ctgtaagcat atctactaca
     3301 tttttcactg catggaagca ggaagggctg ttctgtaaaa atgtaagcag ttcctttgct
     3361 gtttcgttgt agttttctct tgttaattcc ataatgataa ataacttctc cttataatac
     3421 tatcatattt cctaaatatc gcgtttagaa accacgcctg caaaagcaga cggatattta
     3481 ttctgcttac ttttctttca tatatgctac tgccttagaa agtcccatac tgttggaccc
     3541 cttaaacagc aggcagtccc cgaaagctac atggtcatcc aaccattgtt ccagttcttc
     3601 caggcttttt acttctgtca gaggaatatc tccaccggct gcctttaaac cggcaccgat
     3661 ctcttttgcc agttcaccat ataaaactac ctgatctgca ggatgctctt ttaagaaagc
     3721 acctacctcc tgatggaatt ttacagtatc cggtcctaat tccttcatat ctgcaagaac
     3781 agcgatccgc tttccttcac atggcaaaga gccaagtact tcgatccccg ctttcatgga
     3841 aaccgggctt gcgttataac tgtcatcgat cacagtgaca ccattcagat gtacgatctg
     3901 ctgacgtccc ttaaagcctt caaaacggca cagcggttct gctgccattt ccatggaaat
     3961 accgtattct cctgctactg ccagagctgc cagggcattc tgtaccatat gaacacccat
     4021 gacctgaaga cgcagctgta ctttttcatc tccatgaact gctgtaaata caggataacc
     4081 ttcttctaag tgcaggtctt cgcctctgta atcgcagcct tcagaaagtc cgtacagaac
     4141 cttttttctg ccgccttccg gaactacatc tctaagaagg ggatcatctc catttaagaa
     4201 cagagttccc ttttcagaca ttccatcctg gatatgcagt ttctctttta aaatattttc
     4261 ctgagagcct aactgttcaa tatgggctac tccaatattg gtcataaccg cacagtttac
     4321 ctgggctaca tttgcaatac gggtcatttc tcccggctca ctcattccca gctcgatcac
     4381 gccgatctct gcattttccg gaatttccgc aacagtaata ggaacaccta cctggctgtt
     4441 gctgtttcca ggtgttttgt atacaaggaa acctgcactt aaagcagccg ctaccatttc
     4501 acgggtcgtt gtctttccta cgcttccggt aatgccaact aacgggatcg gattgtttct
     4561 tctgcaataa gctccaaaat cctgaagtgc ttttctggta tcaggaacgc agatccaggc
     4621 agctgccttt gcagctgtct ctttttcttt gtcaccttta cactgggtaa gaatggcttc
     4681 ctgtacttcc tcttcggttt tatgacggct ggtaaataca acggttgcgc catttccgat
     4741 ggccatgcag atataatcat gtccgtctgc ccgctcgccg ataataggga caaacaggtc
     4801 atttcctgcc atttttctgg aatccaggct gatatgctct gctaccggct catttccctc
     4861 atgggccaga acgcctcctg ctgcctgcgc aagagccttt gctgttatct ttttcatctt
     4921 tatgccttct ctcccagttc ttttactgtt tcttcgatca cttcacggtc caggaaatga
     4981 tggcggacac cattgatctc ctgataatcc tcatgtccct ttccaatgac agctaccata
     5041 tctcccggct gtgcatgaag gatgctatag cggatggctt ccctgcggtc cgggatctca
     5101 ataaactttc cgcctgtctt ttcaatgctt ccgcggatat ccgcaatgat atcttctact
     5161 ttctcatagc gggagttatc tgctgtaatg atactcagat ctgccatctt tccgccgatc
     5221 tctcccatgg aataacggcg gtccttagaa cggtttccac cgcagccgaa cacgcatacc
     5281 agacgttttg gcttataatc ccggagtgtg gataacaggc tttccatgct cactgcatta
     5341 tgggcgtaat ccacaaggac cgtacatctt tcgctggtat agacgatctc catacgtcca
     5401 tttacacgga tattttctaa ggcatggctg atccgcgcct gatccagtcc caaaaagctg
     5461 catacactga cagccgccag ggcatttacc acattaaaga gacctgggat acccagtctt
     5521 acttccatat catatttacc ggaaatgtgg aactctgttc ccacaaaatc aggctgggat
     5581 acatagcgga tatcagatgc cacaaaatca gcctctttgc tttcttcact gtaagtataa
     5641 agttcacagt ttgctgcctt tatgatctgc ggtgcatgtt catcatcttt atttacaaga
     5701 ccaataccgc acatatttaa cagtctggtc ttatagtaca gatattcctc aaaatcagca
     5761 tgttctcccg gtccaatatg atccggggaa atattggtaa agatggcata gttaaaatac
     5821 agcccatctg tacggtgcat tttaataccc tgtgaggata cttccatcac tgcatactca
     5881 cagccggctt ctaccatctg cgcaaatgcc tggtgcagtt cataagactc cggagtggta
     5941 tttaaggtag gagtcaccac atccccgatc acagtaccgg tggttccgat catacccact
     6001 ttctttcctg cagccagtaa aatagcacgg atcatatggg tagtggtggt ctttcccttg
     6061 gttcctgtta caccgatcat taccagcttt ttagccggat ggtcaaaacg ggcagctgat
     6121 aaaagagcca gggcatgacg gccttcttct accatgacca ctgttacctg ttttaattca
     6181 tcctcagaga ggccgctgtt tttcagctct tcttccaccg gtctttctgt taccaggaca
     6241 cgcacacctg cttttaacac ctggggaatg aaactgtgtc catccacccg gatccctttc
     6301 attgctacaa atacctttcc aggcgctgct tttctggaat catagatcac atcttctgtt
     6361 tctacatcta cgcttccctg aagcagcgta tagtcaagtt ctttcagcca atcacgaaaa
     6421 atcatgtttt aaacctcttg tttctgctat atttgccggt gatcccccaa aaagggggat
     6481 ccacttttag aacaggccgg tgatctttcc gtttaccaca tcaatgcctt ctgcagccgg
     6541 ttttttagga agtcccggca tagtcatgat ggaaccggta agaaccacta caaagccagc
     6601 tccggcacag acataggcat ctcttacatt aatagtaaag ccagccggtc ttccaagctt
     6661 tgtctgatca tcagacaggg aatactgggt ctttgccata catacaggca gattgccaaa
     6721 gcccatttct tcaatgcgct ttaatgcctt ggaagctgcc ggtgcataag atacgccatc
     6781 tgctccgtag atctcagaag caatggttcc gatcttatct tttaatggca tctcatctgt
     6841 ataaagtggc ttaaagtcac ctctcttatt ttccagggta tttaatactt tctcagctaa
     6901 tgccataccg ccttcgccgc cctttgccca gacttctgcc aaggcaaatt cacagcctct
     6961 gtcctcgcag aagcgcttga tataagcata ctcagcttct gtatcggtta tgaaagagtt
     7021 taaggtaaca actaccggaa caccaaattt ctggatattt tcaatgtgct tttcaaggtt
     7081 tacaatacct ctctttaatg catccagatt ttcttcggaa agctggtctt ttggaacacc
     7141 gccattatat tttaatgctc ttacggttgc tactaataca actgcatctg gatggatgcc
     7201 tgctttgcgg cacttgatat ccataaattt ctctgcaccc aggtcagcac cgaaaccagc
     7261 ctctgtaaca acgatatcgc aaagttttaa cgctgtcttt gtagcacgta cactgttaca
     7321 gccatgagcg atatttgcaa atggtccgcc gtgtactaaa gcacctgtat gctctaaggt
     7381 ctggatcata tttggcttta aagcatcctt taacagagct gccatagaac caactgcgtg
     7441 aagatctgct gcagttacag gctctcctgc aaagttgtag gcaacgatga tcttgccaag
     7501 acggtctttt aagtcttcca tatcatttgc aagacagagg atggccatga tctcagatgc
     7561 aacagtgata acaaaatgat cttcacgtac cataccgtca gcctttgcgc caagacctac
     7621 tactacattt cttaaaacgc ggtcattcat atccaggcag cgtttccaga ggatctgtct
     7681 tggatcaatg ccaagtgcat ttccctgctg gatgtgattg tcaagaagag ctgccagcag
     7741 attgtttgca gatgtgatcg catggaagtc acctgtaaaa tgaaggttca gatcttccat
     7801 aggaagtacc tgtgcatagc cgccgcctgc tgctccgcct ttgatgccaa agcacggtcc
     7861 aagggatggt tctcttaagg cgatgatggc atttttgccc atcttgccaa gagcttctcc
     7921 cagacctaca gtaatcgtag tctttccctc accagccggt gtaggattga tggctgttac
     7981 caggaccagc ttgccgtccg gacgtccttt tactttctcc cagagttcat ctgaaagttt
     8041 tgctttatat tttccataaa gctccagatc atcctctcca ataccataag atgctgctac
     8101 ctccttaatt ggctgcatta ccgcttcctg tgcgatctca atatctgttt tcataggtac
     8161 ttcctccttg atgaataatg atatgataat gatattatct gccctgttca agcagttctt
     8221 caaactgctc cgggctcatt aatacttttc tcggcttggt tccttcttct tcgcctacaa
     8281 ctccggcttc tgccagctgg tccataatgc gggccgcccg gttaaaaccg atcttaaaga
     8341 ctctctgcag cataccaatg gatgccttgt ctttttcaat aataaattta ccagcctggg
     8401 caaaatattc atcccgtccg gctgaggaac tgcctccatc cgaagaaggc gcagccacac
     8461 ggctgatcac atcttcatta tactgggcag tcattccctg ttctgttaaa aattctacta
     8521 cccgtcctac ctcttcatca gagacaaagg cgccctgaac acgctgaggt ttcgggaaac
     8581 ctgcaggata aaagagcata tcgccctttc caagcagttt ttccgcaccg ttcatatcaa
     8641 taatggtacg ggagtctaca ccggaagaaa ccgcaaaggc aattctggat ggtacattgg
     8701 ccttgatcag accggtgatg acatttacag atggtctctg ggttgcaatt accagatgga
     8761 taccggccgc acgggctaac tgtgccagac ggcagataga gtcttccacc tctcccggcg
     8821 caaccatcat aaggtctgcc agctcgtcga tgatgataac gatctgaggc agtttctttg
     8881 gctttttatc atctggaata tccgtcagct tgtctacttt ttcgttgtat ccctttaaat
     8941 ctcttacatt acattctgca aacttcttat atcggtctgt catctccgca actgcccagt
     9001 ttaatgctcc ggatgccttt ttcggatctg taacaaccgg gatcagcaga tgagggatac
     9061 cattgtatac acttaactct actaccttcg gatctaccat gatcatcttt acatcctttg
     9121 gatcggattt aaagataata ctcatgatga gggtattgat acagacggat ttaccagaac
     9181 cggtagcacc tgcgatcaaa aggtgtggca tcttggcaat atctgtaaca actacctgtc
     9241 cgccgatatc cttacctact gcaaaggcaa ggcgggattt atggttctta aaagagtcgg
     9301 agtcaagaag ttctcttaag tatacgatat tgttctcctt atttggaact tcaataccca
     9361 ccgcagattt ccccgggatc ggcgcctcaa tacggatatc cgcagctgca aggcttaact
     9421 tgatatcatc tgtaaggcct acgatcttgc ttacctttac cccctgttcc ggcagtaatt
     9481 catatctggt aacagaagga ccgcagctga tattggtaac cgtaacaccg acaccaaagt
     9541 tgtgtaacgt ctgctgcagt ttaatagcag ttgccttata ttcattttca gagaaggcat
     9601 tattatttct caccggtttc tttaaaagat ccgttggcgg gaatatgtat tctttttcta
     9661 cttcctgttt tgcgatctct ttggcaactg cctcagatga ctcttcttcc gaaggcggag
     9721 tgatccgtac cttttcgttg gaagatcgag gtgccataaa aggatctgcc tttggcacag
     9781 atgcagcagc ttctgccgcc cgggtgcttt ccagcttctt cttcaacaga tctgtttctg
     9841 cttcgatcac ttttccggaa gctgtcacta tctgcttctt ctcctccggt acatagatgg
     9901 aaccggaagc agcagccagt ggatcaggag aaacactctc atccagtttt ggatcccagg
     9961 gggactcctc tggctcttct gtgatctctt cccagtcttc tgactgttca aaatttcttt
    10021 tcgtttgagg tttttcctca tcatttatgt gagatttttc ctcatattca gttttttcgc
    10081 tgttgtatac aggtgtctcc gttgctttct cagctgctct ttcctcttct tccagatcag
    10141 ccaggctgac tgtttctttc ggaaggtctt catcccacaa cggttcctca acaggaactc
    10201 tctgtatttc ttttggaagg ggttcttcct cttcttttat cgcatgttca gatacagcag
    10261 atactttctg ctcccaggca gtgggccgtc cggcagggat cacatcagga gccggtatct
    10321 ccatctcctc taaggtccgt cgatcttttg gaacatggat cgtctcatac tgctgttcta
    10381 actcgtcagc aagagcctct gcctcatcct tctcatcctt atttttctca tagccggatg
    10441 gcatctggat cttgcccctg aactcgtctg caggagaagg gatctcctct tcttcctcat
    10501 agactacccg gttctttcct gtagctgccg gtttttcctc cgggatctca tccatctcag
    10561 gttcagcagc ctgctgcttt gtaccaaact cccgttcaaa agcttcctca tcgtattctg
    10621 catcttccgg aacagggcgt ctgccttcat ctacagccgt cagtctggtg gcatttaagt
    10681 tgacaccacg tactctctgt tcttctctta acagtttacg ctcttctgct ctctgggcat
    10741 ggatctcttt tctacggtcc atatcttctc ttgcatactg ataagcccgt ccactgccct
    10801 gcttaactgc cgaaacaaag gatttacctg ttatacatac catgcacagg atcaggatta
    10861 ccaaaaatac cagataagcg cctgctcttc caacaaagaa cataagagcc atagccagcg
    10921 ctccgcctac cagaccgcct ccggcgcctc catttgaagc atctgcatag atctgcatta
    10981 cagatccgcc ctgaggcatc cttccaaaaa gcaactggaa cagtccgcaa agagtgatca
    11041 gcgccccaat cacagctcct aatttaatag ctgcatggat atttccctga tttgataaat
    11101 gaaaacaggt tcctacaaag atcaggatcg gtaaaagata tcccaggatg ccaaacagtc
    11161 ctaactgtat ccccttaagg atctgtccta cgatcccgca taaaccaaag ttacttaaaa
    11221 ataaaaggat ggcaacggca aaagaacaga taatgatcac ttctgccctc ataaacccta
    11281 aatcgtcttc ttcataaacc ggttccggcg cctgtcttga ggaacttctt tttgtgccgg
    11341 atgccgtgga tcgttttctg ctgtttcctg ctgtggtttc tttttttgtc gtacttccct
    11401 taggtcttcc cggtttaccc gttcttccct gctgtttttt tactgaatca gccacaaatc
    11461 atagcctccc atttccataa aacgttctca gagaacatat gttctctgat tttaaagact
    11521 atttacagta tacaaaaaat tcgtaagaat tgcaacgctt ttgatttttc acaaagcttt
    11581 cgtaaaaaac cggtaggcat tatcaaagga aagtttgtct acgatctcac ggctgatccc
    11641 atgttccacc agatattctc cgtatgccgc agcaccataa ggagtgccaa tggatggatc
    11701 acaggtaact ccgccgtcca gatccatgcc ccaggcgatc acatcttctc ctccaagttc
    11761 taacatacgg tcaatgagac gaaggagctg ctccttctgc ccagctccca ggtcatccgg
    11821 ctctgtaaga aatctggaat aaatattgat cccgcaaaga cctcctcttg ccacgatctc
    11881 cttaaactgg tcatcccgca gattgcggct gtgactgcac agggaacgta aattcgagtg
    11941 ggaagctaca aagggtttag tagaacagtt taacagatct tcaaaacctg catcatttaa
    12001 atgggaacag tctacgatca tccccacttc ttccatacgg cgcacagctg cttttccaaa
    12061 atctgtaaga cccatttctg actggaaacc acttcccagt tcattgatcc cgttccaggt
    12121 cagtgtcatc atcttaaaat tcagttttgc aaaatgatct acattttcca gttttccgcc
    12181 aagtgcggca ccactttcaa tcgaacggat aaacgcccat tttccttctt ttaatatcct
    12241 gtccagatca gacatatctt ctactaaagc cgccctgtct gcaacctttc ccacagcctc
    12301 tgtcatatat tcataatatc cattggtaaa atccactgct tcctggcctc ttaaatgatc
    12361 aggaagccac acagccagaa actggactgc tttttctaac ttttcctgtc ccttcatggt
    12421 aaactgggtg gtactattat caaaggtttc attgtgtttt tttaatgctt caatggtatc
    12481 gcagtgtaaa tcaaaaatct tcattctatc ctcccaaaat cgcgcctggc gaacttctct
    12541 ttatagttta cactgcccca ttaaaaatta aaacccctgt ttttttgcac aggactcatc
    12601 aatcatctca tacagctttt ttaatgcttc cctgatccct ccaacttcat taataagacc
    12661 tgcatctact gcttcctgtc ctaccagcac ggttcccaga tcccttgcca gcatttttgt
    12721 attgtgcatt aaagccttta actggtcgta ggcaatatct gcatgggaag aaacaaaatt
    12781 taagatccgg tcctgtatca ttgcaaaata ctcataagtc tgggctgttc caatgaccat
    12841 cccgctcata cggaccggat gtacgatcat ggtccccgaa ggcacgataa aagaataatc
    12901 cgaagcaact gccagaggaa ccccaataga atgactgcct cctaaaacaa gggaaaccac
    12961 cggaatactc atagacgcga tcatctccgc aatagcaagc ccggcatcta catcaccgcc
    13021 ggaggtgttt aaaagcacta agagtccctc cactgtggca tcatcctcaa tctgagccag
    13081 tgcaggaagc acatggtcat attttgtcgt cttggaaccg cctgaagcat tttcatgtcc
    13141 ttccacttct ccaatgatgg ataaaagatg gatctttctc tttttttcat ttccgtccaa
    13201 agtcatctgc ccccagtttt tcaggtgggt atcttctttc tcttcaatct ctttttcctg
    13261 aagttttcgc tcctgtgacg gggctgccgc tttattttcc tgttttcctt cttttttgtc
    13321 cttctgattt ttacccataa aaaagaccgt cctttctgta tctgtattta cagttacata
    13381 aggacagtct ttcctgtttt tttatatttt atttattata gacccactat acaatggtcc
    13441 agcctccatc tactaacaga agctgtcctg ttacataact ggatgcatct gatgcaagga
    13501 acagcatagc accgttcaac tcgccaggat ttcctctgcg tcccattggg cagttgttgc
    13561 ggatagaagc ctcatatact tcatttgcaa cctttgcaga catttcactt gcaaaatatc
    13621 caggacctaa aacattgacg gtaatgttat tctttgccca ctctgcagcc agtgccttgg
    13681 acatcattaa aactgcgccc ttggcagtgg aataaccgga acgcggaaca ccattcatgg
    13741 ttacctggca gtggaaggaa ccgatgttga tgatctttcc gtaattctgg gcgatcataa
    13801 cctttcctac ttctcttgca aagaagaaaa cgccgtctac attggtcttc attacattgt
    13861 accactgttc atcggtagtc tctacaacag gggttgcaaa agaagctgct gcgttgttga
    13921 tcaggatatc aatacgtccg aattctttta ccaccttgtc tacggaatta cggatctgtt
    13981 ctgtatcagt tacgtcacat ggcagcacca gacattttac gccgtaagct tctacttctt
    14041 tctttacttc ttccaggcgc tctactctgc gggctgcgat ggctacatta catccctgct
    14101 ctgccagaca aagcgcaaac tgacgtccca gaccggaaga agctcctgtt acaactgcat
    14161 acttgccagc tacatcaaaa tagtttttca tgattttatt tcccctttct ggatctgatc
    14221 ttttaacagg atccttgaaa ccttgccatt ggcgttttca ggaagctgtg aaaagaagat
    14281 tacttttctt ggctttttat aacttgctat gtgctgctta caccatgcca caatatcatc
    14341 ttctgtcagt ttagaacctt cttttaagac gatacaggca gtgacctgct ctccccagac
    14401 cggatccggc attcccagca cacagacctg ttttacatcc tgtttcaaag aagcgataca
    14461 atcctccgct ggtagccgga tataaatttt ccgattattt aaaatacact ctgccccatg
    14521 cactgatcca gtttttatgt atctggtttt taatcccctt attcttcccg ctggtataaa
    14581 taaaagagac cacctgaaaa agcttttaaa cctctttcag gtgatctctt ttttaatact
    14641 atttcttgct atttcatttt tatctggtca tgatccagct
//
LOCUS       DBMDUA010000056        14671 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2242_length_14671_cov_3.030268, whole genome shotgun sequence.
ACCESSION   DBMDUA010000056 DBMDUA010000000
VERSION     DBMDUA010000056.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 14671)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14671)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14671
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2242_length_14671_cov_3.030268"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="ACZPCL_06060"
     CDS             complement(<1..1280)
                     /locus_tag="ACZPCL_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HIR1162075.1"
                     /translation="MNTVNDGSTEKMTVNQKLELLRTKMKERGMDAYMVPTADFHESE
                     YVGDHFKCRQFLTGFTGSAGIAVVTMKEACLWVDGRYFVQAAVQLQGTSVKMMKMGQE
                     GVPSVAGYLEAEVPENGCLGFDGRVVNALTGIDLEKRLKEKNARILCSDDLINEIWED
                     RPALSMEPAWALHEKYAGKTAEEKLKELRAEMKKEHTDMYVLTSLDDIAWLLNIRGND
                     IAYNPVVLSYVIVTEDKLYLFVNEEVLHGEAYPYIDNYHNIDMLKYLEDLGTEILPYD
                     DIYKMATDITGHKILMEKQHINYALYNILKDKNELVDKMNPTSDAKAVKNEVEIENEK
                     KAHIKDGVAVTRFIYWLKHHIGKERLTEMSVQDKLEEFRKEQEGYIEPSFATISAYGA
                     NAAMCHYSATKESDTVLEDHGFYLLSISLLPFPDS"
     gene            complement(1324..2136)
                     /locus_tag="ACZPCL_06065"
     CDS             complement(1324..2136)
                     /locus_tag="ACZPCL_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HIR1162076.1"
                     /translation="MINKTTGSKTDKKRIVSFDLDMTLLDQATWKIPDSAIEAVEMLR
                     KDSIIVIASGRNMDGMVSVGYKQQIRPDAVIHMNGTRVVAGDELLYEHFMDKELLKRL
                     LSYGEEREIAVGLTIDDKDYFVNPAEVERIDKIRWGESMRNFQDPWKMLELPVRTLAY
                     IGEADPVSDMEAHFPELKFPLFSSRLGADIIEVQASKANGLVRLCEYYGMDISQTVAF
                     GDSMNDYEIVQAAGTGIAMGNSVQKLKDTADYVTDDIARDGVWKACRHFGWI"
     gene            2231..2304
                     /locus_tag="ACZPCL_06070"
     tRNA            2231..2304
                     /locus_tag="ACZPCL_06070"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2265..2267,aa:Pro,seq:ggg)
     gene            complement(2335..2553)
                     /locus_tag="ACZPCL_06075"
     CDS             complement(2335..2553)
                     /locus_tag="ACZPCL_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162077.1"
                     /translation="MMMPGQKGRKSGAGLPAGGFLNLGPAKNMSKKPLFEEKIRGLGM
                     FLELRECKARSNSVSSGTVIKKIRASDE"
     gene            complement(2782..4137)
                     /locus_tag="ACZPCL_06080"
     CDS             complement(2782..4137)
                     /locus_tag="ACZPCL_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1162078.1"
                     /translation="MKTNTDLFEKVPVPKAVATMAVPTMISMLVVVIYNMADTFFIGQ
                     TQDPLQVAAVSLATPVFMIFMALGNLFGIGGSSAISRALGEKHKDRAWHISSFCCYGA
                     LGLGVIVAVCSVLGMEGILGLIGASENTIGFARKYLLIISIGAPTILFSTAFANILRG
                     EGAARESMVGNLLGTIINIVLDPVMILMLGWGVTGAALATIIGNIAACLFYLQYFLRK
                     KSTLSISWKYFKVGEGIAKSVVAIGIPASLNNILMSFANIILNQALVGYGDTPVAAMG
                     VALKSNMLVVLLQIGLCVGIQPLIGYNYGAGNKKRLMQVFKFTGIVSVIMGTILTLFM
                     MVARKSLVQVFINDAQVITYGIQMVIALQLSAPFLGILFLCINTIQGMGKAIPSLLLT
                     VCRQGLIFIPLIFILNHMFGLEGVIYAQPAADYLSIVVAILICTHLFRTMEHRNESVE
                     A"
     gene            4416..4850
                     /locus_tag="ACZPCL_06085"
     CDS             4416..4850
                     /locus_tag="ACZPCL_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1162079.1"
                     /translation="MLVSTKGRYALRIMIDLAEHQRDGYIPLKETAGRQGISEKYLEG
                     IIKILVKDKMLTGVRGKGGGYKLTKSPDQYTVGSILRLTEGSLAPVSCLESPDALSCE
                     RMDCCRTLPMWRGLDHLINNYLDNITLADLIQPEEPWSNYVI"
     gene            complement(5052..5867)
                     /locus_tag="ACZPCL_06090"
     CDS             complement(5052..5867)
                     /locus_tag="ACZPCL_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HIR1162080.1"
                     /translation="MRRRFAVAAAALALSAALTVPAYAGTWKYVNDQWKYQKGTNKFA
                     YNEWIKDNGKYYYIGNDGIMKIGWQQIDGQWYYLDQTGAMQIGWLKSDDGKWYFLYPN
                     GAMAVNTVIDGRTIGEDGVWVPNEGDTEPANSMDLENGYLVQNLEGITKKDYTIIASG
                     KTAAGSRWGNAIRLKGKGSYVQCNTNGEYRMLSGVFGPSSQFDSALLARVTVYGDDDQ
                     VLYTSPDIHYNEKNVYFGVDVSGQKQIRVEVSLLKDNEWDDPVILFDGLSLYK"
     gene            complement(6049..6561)
                     /gene="nrdG"
                     /locus_tag="ACZPCL_06095"
     CDS             complement(6049..6561)
                     /gene="nrdG"
                     /locus_tag="ACZPCL_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIR1162081.1"
                     /translation="MNYGNIKECDIADGPGVRVTLFVSGCRHHCKGCFNSETWNFQYG
                     VPFTEETQDKILKLLEPGYIQGFTLLGGEPFEPENQAVLVHLLKKIRETYPQKDIWCY
                     SGYLFDQDMQPGGSVYTEYTKEMLRQIDVLVDGEFVEAKKDLTLIFRGSSNQRLLRLK
                     DGEFAGMWED"
     gene            complement(6752..8446)
                     /locus_tag="ACZPCL_06100"
     CDS             complement(6752..8446)
                     /locus_tag="ACZPCL_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HIR1162082.1"
                     /translation="MGIMTIDGQSIEFTDEPNVLSVIRKAGIDIPTLCYHSELSIYGA
                     CRLCTVENDRGKTFASCSEKPKDGMVIYTNTPRLMRYRKLILELLLAAHCRDCTTCIK
                     SGECHLQELAHRMGVHEIRFENVREQQPIDTSSHAIIRDPNKCILCGDCVRMCDNVQN
                     INAIDFAYRGTDAQVMPAFNKKIAETDCVGCGQCRVVCPTGAISIHTNIDVVWEALAD
                     KNTKVIAQIAPAVRVAIGDHFGYAKGENVMGKLVGVLHRLGFDEVYDTSYGADLTVVE
                     ESKEFIERFTSGQKMPLFTSCCPAWVKYCETKYPEFVPNLSTCRSPQQMFGAVVREYY
                     KDPEKNEGKKIVSVSIMPCTAKKEEILRPESFTNGKQDVDYVLTTTEVVRMIRKSGIV
                     FDKVEIEAADVPFGIGSGSGVIFGVTGGVTEAVLRRLQQGHSRVDMEAIKKSGVRGDE
                     GIKTLTYDYNGREIRAAVVNGLANADKLLQQIKNHEVEYDFVEVMACRRGCIMGGGQP
                     VNAGPRTRKARMKGLYDTDVNTQIKKSNENPMILSLYDSLLKGKEHELLHRNFSGK"
     gene            complement(8462..10330)
                     /gene="nuoF"
                     /locus_tag="ACZPCL_06105"
     CDS             complement(8462..10330)
                     /gene="nuoF"
                     /locus_tag="ACZPCL_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR1162083.1"
                     /translation="MKIENRMQLHLWREECKEKRQKETCRVLICGGTGCLAGGSDKIY
                     ARIKELTANMSNVTVKFGEEIAHVGLKMSGCHGFCEMGPLVRIEPYNYLYLKVKLEDC
                     EEIFEKTILRGEPVTRLMYEDSGRVYETQEEIPFYAKQTRLVLKNCGHIDAEHIEDAM
                     AVGAYEAFEKAVFEMTPEAVIKTVTDAGLRGRGGAGFPAGKKWTQVASQKEKIRYVVC
                     NGDEGDPGAFMDRSVMEGDPHRMIEGMMLAAYAVQAQEGYIYVRAEYPLAVRRLQIAI
                     AQAEEKGLLGDNILGTGFSFRLHINRGAGAFVCGEGSALTASIEGKRGMPRVKPPRTV
                     EQGLWGKPTVLNNVETYANVPMIIKNGAGWYSRIGTPESPGTKAFALTGNVKNTGLIE
                     VPMGISLREIIFDIGGGIKDNKKFKAVQIGGPSGGCLVESQLDSKMDFDSLSKIGAMI
                     GSGGLVVMDEDTCMVEVARFFMNFTQRESCGKCVPCREGTKRMLEILERIVAGNGKMS
                     DLDELEELADMIENTALCGLGKSAPKPVISTIHAFRKEYEEHILDKKCRAHVCSGLRV
                     FKIDPEKCKGCSKCARNCPVNAISGKVKSPFVIDNEKCVKCGSCMDNCAFGAIYTE"
     gene            complement(10327..10809)
                     /locus_tag="ACZPCL_06110"
     CDS             complement(10327..10809)
                     /locus_tag="ACZPCL_06110"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I 24 kDa subunit family protein"
                     /protein_id="HIR1162084.1"
                     /translation="MLDTSYYEKTDQIIAQYSCEEKSLIPIIQGIQEEYRYLPPELLT
                     YVAGKLGISEAKAYSVASFYENFSFEAKGKYVIKVCDGTACHVRKSIPILEGLYKELG
                     LGKKKHTTDDQLFTVETVSCLGACGLAPAVMVNDEVHPKMTPEKMSELIAKLREDEKV
                     "
     gene            complement(10986..13175)
                     /gene="nrdD"
                     /locus_tag="ACZPCL_06115"
     CDS             complement(10986..13175)
                     /gene="nrdD"
                     /locus_tag="ACZPCL_06115"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIR1162085.1"
                     /translation="MSVEVKTNVIKRNGEEVHFNSQKIVNAVHKANEEVDRLHQMNDY
                     QIRAIADNIANKVQESTHAVNVEDIQDMVETGIMAMRGYEVAQKYVRYRYKRELTRKS
                     NTTDNGILSLLDNINEEVNQENSNKNPVINSTQRDYMAGEVSKDLSRRVLLPEKIVRA
                     HEEGIIHFHDTDYFAQREHNCDLINLEDMLQNGTVISETLIEKPHSFFTACNVTTQIV
                     AQVASNQYGGQSFTLAHLAPFVDISRKKIRKNVIEERKECGEPMDEAIIDKVTERRLR
                     EEIKSGIQTIQYQLITLMTCNGQAPFVTVFMYLDEVPEGQTRKDLSMIIEEVLVQRMQ
                     GVKNEKGVWITPAFPKLIYVLDEDNISEDAPYWYLTELAAKCTAKRMVPDYISAKIMK
                     ELKQGDVYPCMGCRSFLTVEDSQRNADGSHKYYGRFNQGVVTINLVDVACSSEGDMDR
                     FWEILDERLELCHEALRCRHERLLGTISDVAPILWQNGALARLKKGEKIDKLLFNGYS
                     TISLGYAGLYEMCMRMLGKSHTDPEARPFALKVMQRLNDKCKEWKAAENISYSVYGTP
                     MESTTYKFAKCLQKRFGIIPGVTDKNYITNSYHVHVSEKIDAFSKLKFESDFQKLSPG
                     GAVSYVEVPNMQNNIPAVLSVMKFIYNNIMYAELNTKSDYCECCGYDGEMVIKEEESG
                     KLIWECPNCGNKDQNKMFVARRTCGYIGTQFWNQGRTQEIRDRVLHL"
     gene            complement(13524..13604)
                     /locus_tag="ACZPCL_06120"
     tRNA            complement(13524..13604)
                     /locus_tag="ACZPCL_06120"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13568..13570),aa:Leu,seq:tag)
     gene            complement(13659..13743)
                     /locus_tag="ACZPCL_06125"
     tRNA            complement(13659..13743)
                     /locus_tag="ACZPCL_06125"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13707..13709),aa:Leu,seq:taa)
     gene            complement(13754..13826)
                     /locus_tag="ACZPCL_06130"
     tRNA            complement(13754..13826)
                     /locus_tag="ACZPCL_06130"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13791..13793),aa:Val,seq:tac)
     gene            complement(13861..13934)
                     /locus_tag="ACZPCL_06135"
     tRNA            complement(13861..13934)
                     /locus_tag="ACZPCL_06135"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13898..13900),aa:Asp,seq:gtc)
     gene            complement(13974..14047)
                     /locus_tag="ACZPCL_06140"
     tRNA            complement(13974..14047)
                     /locus_tag="ACZPCL_06140"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14011..14013),aa:Ile,seq:cat)
     gene            complement(14080..14152)
                     /locus_tag="ACZPCL_06145"
     tRNA            complement(14080..14152)
                     /locus_tag="ACZPCL_06145"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14117..14119),aa:Thr,seq:tgt)
     gene            complement(14177..14248)
                     /locus_tag="ACZPCL_06150"
     tRNA            complement(14177..14248)
                     /locus_tag="ACZPCL_06150"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14213..14215),aa:Glu,seq:ttc)
     gene            complement(14283..14355)
                     /locus_tag="ACZPCL_06155"
     tRNA            complement(14283..14355)
                     /locus_tag="ACZPCL_06155"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14320..14322),aa:Asn,seq:gtt)
     gene            complement(14522..>14671)
                     /locus_tag="ACZPCL_06160"
     rRNA            complement(14522..>14671)
                     /locus_tag="ACZPCL_06160"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         3842 a         3813 c         2606 g         4410 t
ORIGIN      
        1 gaatcaggaa aaggtagtaa tgaaatggat agaagataaa agccatggtc ttccagtacc
       61 gtatcacttt cctttgttgc agaatagtga cacatagccg cattcgcccc ataagcagag
      121 attgtagcaa agcttggctc aatatagcct tcctgctctt tcctgaattc ctctaactta
      181 tcctggacac tcatctctgt aagacgctct tttccaatat ggtgctttaa ccagtagata
      241 aaacgggtaa cagctacacc gtccttaata tgagctttct tctcattttc aatctcaacc
      301 tcattcttta ctgctttcgc atcagaagtg ggattcatct tatcaaccag ttcattctta
      361 tcctttaata tgttatacaa agcataattg atatgctgct tctccataag gatcttatgt
      421 cccgtaatat ctgttgccat tttatagata tcatcataag gcagtatttc tgtccctaaa
      481 tcttccagat atttaagcat atctatatta tgataattat ctatatatgg atatgcttct
      541 ccgtgaagca cttcttcatt tacaaacaga tacaatttat cctctgttac gatcacgtag
      601 gaaagcacta ccggattata agcaatatca tttcccctga tatttaacag ccatgcaata
      661 tcatccagcg atgtcagaac atacatatct gtatgctcct tcttcatttc tgcacgaagc
      721 tcttttaatt tttcttctgc tgtctttcct gcatatttct catgtaatgc ccatgccggc
      781 tccatggaca gtgccggacg gtcttcccag atctcattga taagatcatc actgcaaagg
      841 atccttgcat ttttttcttt caggcgcttt tccagatcaa taccggtcaa tgcattgact
      901 actcttccgt caaagcccag acagccattt tccggcactt ctgcttccag atatccggct
      961 acagaaggca cgccttcctg tcccattttc atcatcttta cagaagttcc ctgaagctgt
     1021 actgccgcct ggacaaaata tcttccatct acccacaggc atgcttcctt catggtcact
     1081 actgcaattc ccgcagaacc ggtaaaaccg gtcaggaact gtctgcattt aaaatgatct
     1141 cccacatatt cggactcatg aaaatcagca gtaggcacca tataggcatc catgccacgt
     1201 tccttcatct ttgttctgag caattctaat ttctgattca cggtcatttt ctccgttgat
     1261 ccatcattta ctgtattcat attccttctc ctcctgtact cacacatggc tgcatatttt
     1321 cagctatatc catccaaaat gtctgcatgc cttccagact ccatcccggg caatatcatc
     1381 tgtcacataa tcggctgtat ctttcagctt ctggactgaa tttcccatag cgatcccggt
     1441 tccagctgcc tgaacgatct cataatcatt catgctgtct ccaaatgcca ccgtctggct
     1501 tatatccatt ccatagtatt cacataaacg taccagtcca tttgccttgg atgcctgtac
     1561 ttctatgatg tccgccccta accggctgga aaataatgga aatttaagtt ccggaaaatg
     1621 tgcctccata tcagacaccg gatccgcttc tccaatataa gctaacgtac gtaccggcag
     1681 ttccagcatt ttccagggat cctgaaaatt acgcatgctc tctccccagc ggatcttgtc
     1741 gattctttct acctctgccg gattcacaaa gtaatcctta tcatcgatcg tcagccccac
     1801 ggcaatttcc ctttcttccc cataggaaag caggcgtttt aaaagctctt tatccataaa
     1861 atgctcatat aacaattcat ccccggcaac cacacgggtt ccattcatat ggattactgc
     1921 atctggccgt atctgttgtt tgtagcccac actgaccatt ccatccatat ttctgccgct
     1981 ggcgatgaca ataatactgt cttttcgcag catctccacc gcttctatgg cactgtccgg
     2041 tatcttccat gtagcctgat ctaaaagtgt catatccaga tcaaaactta cgatcctttt
     2101 cttatctgtt ttgctgcctg tcgttttatt gatcatctga aaaactcctc attgcggtat
     2161 acattaaaaa aacaaaatcg gggcttttca aacagcctct tttctgttat actataatag
     2221 ttatgaacat cggagtatgg cgtagcttgg tagcgcgctc ggctgggggc cgagaggtcg
     2281 caggttcaaa tcctgttact ccgatttatt ttttatcaag ggtttttgat gattttactc
     2341 atcagaagct cttatttttt taatcaccgt tccagatgat accgaattgc tccgtgcttt
     2401 gcactcccgc aattctaaaa acattcctaa tccccttatt ttttcttcaa ataatggctt
     2461 cttactcatg tttttagcag gtcccaggtt caaaaatcct cctgcaggca agcctgcgcc
     2521 ggatttccgc cctttttgcc ctggcatcat catatagatt ctatcgaaaa aatattaaat
     2581 tatcaatatt tttcacctgc aaatccaaaa acttcttaaa tgttctctcg gaatctttct
     2641 gtacctgact ttgcgagaag attttttgtc agccgcaaag gcaagtccat aaaagactcc
     2701 ggtaggacat ataacagaaa aggagtgagc atgccatttc atatactcac tcccaaatcc
     2761 tgtcttactt atttgtacct tttatgcctc aacactctca tttctatgct ccatagtcct
     2821 gaacaaatgg gtacagatca ggatcgctac tacaatggac agataatccg ctgcaggctg
     2881 cgcataaata acgccttcca gtccaaacat atgatttaaa ataaagatca acgggataaa
     2941 gatcagtccc tgacggcaga ctgtcaggag caatgacggg atcgcctttc ccattccctg
     3001 gatggtattg atgcacagga ataaaatccc taaaaacggc gctgacaact gcagtgcaat
     3061 aaccatctgg ataccataag tgataacctg ggcgtcattg ataaacacct gaaccagtga
     3121 ttttcttgcc accatcataa acagggtcag gatcgttccc atgatcaccg atacgatgcc
     3181 tgtaaactta aacacctgca tcagacgttt cttatttccc gcaccataat tatatccgat
     3241 cagcggctga attcccacac aaagtccgat ctgcagcagc actaccaaca tattggattt
     3301 caaggcaacg cccattgcag caaccggtgt atctccatat cctactaatg cctgattaag
     3361 gataatattt gcaaaactca ttaaaatatt gtttaaagat gcaggaattc caatcgctac
     3421 tacgctcttt gcaattcctt ctcccacttt aaaatatttc cagctgatag acaaagtgga
     3481 ttttttcctg agaaaatact gcagataaaa caggcatgct gcaatatttc caatgatcgt
     3541 agccaatgct gcgcctgtta ctccccagcc taacatcagg atcataaccg gatccagcac
     3601 aatatttata atagttccaa ggaggtttcc caccatagac tctctggccg cgccttctcc
     3661 tcttaagata ttggcaaatg cagtggaaaa caggatcgtt ggcgcaccaa tggaaatgat
     3721 cagcagatat tttctggcaa aaccaatggt attttcactg gctccgatca gtcccaaaat
     3781 cccttccata ccaagtacag aacagacagc cacgatcact cccagaccta acgcaccata
     3841 acagcaaaag gaactgatat gccatgcccg gtctttatgc ttttcaccta atgccctgga
     3901 tatagccgag cttccaccga tgccaaacag atttcccagc gccataaaga tcataaatac
     3961 cggtgttgcc aaagatacag ccgcaacctg cagtggatcc tgtgtctgac caatgaaaaa
     4021 ggtatctgcc atgttgtaaa tgacaactac cagcatagat atcatggtag gaacagccat
     4081 agtagccact gctttaggta ccggaacttt ttcaaacaga tccgtattgg ttttcataca
     4141 tttttcctct ctttttccca cattttctcc attgtatgct ttttacattt tgtcttattt
     4201 ttaacgtatt taagccattt tgcgtaagca ttctgccatt ctaaacacaa tctgtatatt
     4261 tgtcaactgt tatttttttg ccgaggttct gctgaaattc tgtcatccca ttttcctgtt
     4321 actgagaaat tcatattcca actttctgca tttttcattg ctctgccact aaccttgttg
     4381 tataataggt ttataataag aaatggagaa tgcgtatgtt agtttcaaca aaagggcgtt
     4441 atgcactgcg tatcatgatc gacctggcag aacaccagag agacggctat attccattga
     4501 aggaaacagc cgggcgccag ggcatttcag aaaaatacct ggaagggatc attaagatcc
     4561 ttgtaaaaga taaaatgctt acaggtgtcc gcggcaaagg cggcggatac aaacttacca
     4621 aatcaccgga tcagtataca gtaggaagta tcctgcgcct tacggaaggc tcccttgccc
     4681 ctgtatcctg tctggaatcc ccggacgcac tttcctgcga gcgcatggac tgctgccgta
     4741 cactccctat gtggagaggc ttggatcacc tgatcaacaa ctacttagac aatatcactc
     4801 ttgctgacct gatccagccc gaagaaccat ggagcaatta tgtaatctag tccaaaagaa
     4861 aaacaagcgc caccaaaggt ggcatttgtt tttcttggac gattaacgag ggttccgtct
     4921 gcgtcagcag acaatagagc gcgatagcgc gtgaacccga gtgtacatat atacataatc
     4981 tccaaaaaag agtatgccac cgggaatgga actccatccc gaaagcatac tctttttcat
     5041 ttcatctttt cctacttata aagagacagt ccatcaaata aaataaccgg atcatcccat
     5101 tcattatcct ttaacaggga aacttctacg cggatctgct tctgtccgga tacatccaca
     5161 ccaaaatata cattcttttc attgtaatgg atatcaggtg atgtatacag tacctggtca
     5221 tcgtcaccgt aaacagttac tcttgcaaga agggcgctgt caaactggga agaaggacca
     5281 aatacgccag ataacattct gtattcacca tttgtattgc actgtacata gcttcccttg
     5341 ccttttaagc ggatcgcatt tccccagcgg gatccagcgg ctgtcttacc ggaagcaatg
     5401 atcgtgtaat ctttctttgt aatgccttcc aggttctgta ccagataacc attttccaga
     5461 tccatactat tagctggctc tgtatctccc tcatttggaa cccatacgcc atcttcgcca
     5521 atggttcttc catcgatcac tgtgtttaca gccatagcac cgtttggata caggaaatac
     5581 cattttccat cgtcactctt taaccatcca atctgcattg caccagtctg gtccagataa
     5641 taccactggc catcgatctg ctgccagccg atcttcataa taccgtcatt accaatataa
     5701 taatattttc cattatcttt tatccactca ttgtatgcaa acttgttggt acccttctgg
     5761 tatttccact gatcatttac atatttccat gttcctgcat aagccggaac tgttaaagcc
     5821 gcacttaatg ccagcgctgc cgctgcaacc gcaaaacgtc ttctcatttc ccattctcct
     5881 ttcatctgca ggctttattc ctgcgctata ttaaccgctc atactgtctg ttttcgtaca
     5941 ctttattata attcaacttt gctttttatg caacagtgcc tgccatctgt tcacagaata
     6001 tccacggttt tactgggttt tctggacaat catccgctta tctgtcactt aatcttccca
     6061 cattcctgca aattcaccgt cttttaaacg caaaagtctc tgattggaac ttccccgaaa
     6121 gatcagggtc agatccttct ttgcttccac aaactcacca tctaccaaca catcgatctg
     6181 acgcagcatt tcttttgtat attccgtgta aacagagccg cctggctgca tatcctgatc
     6241 aaacagatat ccgctgtaac accagatgtc tttttgtgga taagtctccc tgatcttctt
     6301 taaaaggtgg acaagtaccg cctgattttc cggttcaaaa ggttctcccc caagaagggt
     6361 aaaaccctga atatatcccg gttccagtaa ttttaaaatt ttatcctgcg tctcctctgt
     6421 aaaaggaacc ccatactgaa aattccaggt ttccgaatta aaacagccct tacaatgatg
     6481 acggcagcca gacacaaaca atgttaccct tacaccagga ccatctgcaa tatcacattc
     6541 ttttatattt ccataattca tctgtcattt ctccatttct gttaaatata ctctgcggat
     6601 ctaaataaca taactgaaca agaagtttat ctttatagta tacaccacat cattctcctg
     6661 cacaagaaaa aagcaaagcc cggaaatcac ttccaggctc tgccctttta ttttgtaacg
     6721 tgttcacact cacaaacccg cacttcgtgc tctactttcc cgagaagttt ctatgcagca
     6781 gctcatgctc cttccccttt aaaagtgagt cataaagtga aaggatcatc gggttttcat
     6841 ttgacttctt gatctgggta tttacatctg tatcatacag tcccttcatt ctggcctttc
     6901 tggttcgcgg tccggcattt accggctggc cgccgcccat gatacaacct ctgcggcatg
     6961 ccattacttc cacaaaatca tactccacct catggttctt gatctgctgc aacagcttat
     7021 ccgcatttgc cagaccattt actactgccg cccggatctc tcttccatta taatcatagg
     7081 tcagcgtctt aataccttca tcaccccgga ctccgctctt tttaatggct tccatatcca
     7141 ctctgctgtg tccctgctgc aatctgcgaa gtactgcctc tgtgacaccg ccggtaacac
     7201 caaagatcac accgcttccg gaaccaatac caaaaggcac atcagcagcc tcgatctcta
     7261 ccttatcaaa tacaataccg gacttgcgga tcatccttac tacttctgtt gttgttaata
     7321 cataatccac atcctgtttt ccgttggtaa aggactccgg ccgcaggatc tcttccttct
     7381 ttgctgtaca cggcataata gaaacagata ctatcttctt tccttcgttc ttttccggat
     7441 ccttataata ttcacggact accgcaccaa acatttgctg aggagaacgg caggtggaaa
     7501 ggtttggcac aaattccgga tattttgtct cacaatattt cacccatgca ggacagcagg
     7561 atgtaaataa tggcattttc tgaccggaag taaaacgctc aataaattcc tttgactctt
     7621 ccacaactgt cagatccgca ccataagaag tatcataaac ctcatcaaag cccagtctgt
     7681 gaagtacacc taccagcttg cccattacat tttcgccctt tgcatagcca aaatgatctc
     7741 caatggccac acgtacagcc ggtgcgatct gagcgatcac ctttgtattt ttatccgcta
     7801 aagcttccca taccacatcg atattggtat ggatactgat agcacctgta ggacagacaa
     7861 cccggcactg gccgcagcca acgcagtctg tttctgcgat cttcttatta aatgcaggca
     7921 ttacctgtgc atctgttcca cggtaagcaa aatcaatggc attgatattc tgcacattgt
     7981 cgcacattct tacacagtca ccgcaaagga tacatttatt aggatccctg ataatagcat
     8041 gggaactggt gtcgataggc tgctgctctc ttacattttc aaaacggatc tcatgaacac
     8101 ccatgcgatg agccagttcc tgcagatgac attctccact cttgatacag gtagtacaat
     8161 ctctgcagtg ggccgctaaa agcagttcca ggatcagttt gcggtaacgc atcagccttg
     8221 gtgtattcgt atagatgacc attccgtctt ttggtttttc tgaacaggag gcaaaggtct
     8281 ttcccctgtc attttccact gtacaaagac gacaggctcc atatatagat aattctgaat
     8341 gatagcacaa tgtagggatg tcgatcccgg cttttcttat tactgacaat acattgggtt
     8401 catctgtaaa ttcaatggac tgtccgtcaa ttgtcataat tcccataaat ttccatcctc
     8461 cctactctgt atagatcgcg ccaaaggcac agttgtccat acagcttccg cattttacac
     8521 atttttcatt gtcaatgaca aatggtgact tcacttttcc ggaaatggca tttaccggac
     8581 agttccttgc acacttggag cagcctttac acttctcagg atcgatctta aatacccgaa
     8641 gcccggaaca gacatgtgca cggcatttct tatccagaat atgctcctcg tattccttac
     8701 ggaacgcatg aatggtactg atgaccggct ttggcgcgct ctttcccaat ccgcaaaggg
     8761 cagtattttc gatcatatct gccagctctt ccagctcatc cagatcgctc attttcccgt
     8821 ttcctgcaac aatacgctcc aagatctcca acattctctt ggttccttct ctacatggaa
     8881 cacattttcc gcagctttct ctttgggtga aattcataaa gaaccgggca acttccacca
     8941 tacaggtatc ttcatccata acaaccagac cgccggatcc gatcatggct ccgatctttg
     9001 aaagggagtc aaaatccatc ttgctgtcaa gctgtgactc caccagacag cctccggaag
     9061 gaccaccgat ctggactgcc ttaaactttt tattatcctt aatgccaccg ccaatatcga
     9121 aaatgatctc acgaagactg atacccatag gaacttcgat cagacctgta ttcttaacat
     9181 ttccagtaag tgcaaatgcc ttggttcccg ggctttccgg tgtaccgata cggctgtacc
     9241 agcctgcgcc atttttaatg atcataggaa cattggcata ggtttctacg ttatttaata
     9301 cagttggttt gccccataag ccctgttcta cggttctagg cggttttacc cttggcatac
     9361 ctcgttttcc ctcaatagaa gcggtcagcg cagatccttc gccacataca aaggcaccgg
     9421 cgcctctgtt gatatgcagt ctgaaactaa agccggttcc caggatatta tcgcctaaaa
     9481 gtcccttttc ctctgcctgg gcaatagcga tctgcagtct gcgcactgcc agtggatatt
     9541 ccgcacggac gtaaatatat ccttcctggg cctgcactgc ataagccgct aacatcatgc
     9601 cttcgatcat acggtgggga tcaccttcca taacacttcg gtccataaat gcacctggat
     9661 cgccttcatc accattgcag actacatagc gtatcttttc cttctgactt gccacctggg
     9721 tccatttctt accagccggg aaaccagcgc cgcctcgtcc tctcagtcct gcgtctgtca
     9781 cggtctttat aacagcttcc ggagtcattt caaaaacagc tttttcaaaa gcctcataag
     9841 cgcctactgc catggcatct tctatatgct ctgcatcaat atgtccacaa tttttaagca
     9901 ccagacgggt ctgctttgca tagaaaggga tctcctcctg ggtttcatat acacggccgc
     9961 tgtcctcata catcagcctt gttaccggct ccccgcgaag gatcgttttt tcaaatattt
    10021 cttcacagtc ttccagcttt acctttaaat acaggtaatt gtatggttca atacgaacta
    10081 aaggacccat ttcacagaaa ccgtgacatc cgctcatctt aagtcccaca tgtgcgatct
    10141 cttccccaaa ttttaccgtc acattgctca tatttgcagt cagttcctta atacgggcat
    10201 agatcttgtc ggaaccgcct gccagacatc ctgttcctcc gcagatcagg actctgcagg
    10261 tttctttctg gcgtttttcc ttacattctt ctctccacag atgaagctgc attctgttct
    10321 ctattttcat accttctcat cctcccgcaa ttttgcgatc agttcactca ttttctccgg
    10381 tgtcattttc ggatgtactt catcattgac cattacggca ggcgccagtc cgcaggctcc
    10441 caggcaggaa acagtttcca ctgtaaacag ctggtcatct gttgtatgtt tctttttgcc
    10501 aagccccagt tccttgtaaa gtccctccaa aataggaatg gacttacgca catgacaggc
    10561 tgttccgtca cagactttga tcacatattt tccctttgcc tcaaaagaga aattctcgta
    10621 aaagcttgct acactgtaag cctttgcttc tgagatcccc agtttccccg caacataggt
    10681 cagaagctcc ggcggcagat aacgatattc ctcctggatc ccctggataa tagggatcag
    10741 ggatttttct tcacagctat actgtgcaat gatctggtct gtcttctcat aataagatgt
    10801 atctaacatt gtacccctcc ttttgtgcat ttcacgtata atcaattata catgagaaaa
    10861 tgttttgtgc atagtgctta ttgatatttt tttgacgaaa ttctcaataa gtataacaaa
    10921 ctacctgaat aagaaaaaag aagccccaga atttactctg agacttctct ttaagttcat
    10981 tgattttaca gatgtaatac tctgtcccgg atctcctggg tccttccctg gttccagaac
    11041 tgtgttccaa tataaccgca ggttcttctt gctacgaaca tcttattctg atccttgttt
    11101 ccacaatttg ggcactccca gatcagtttt ccggactctt cctctttgat gaccatttca
    11161 ccatcgtaac cacagcattc gcagtaatcg ctcttggtat ttaactctgc gtacatgata
    11221 ttgttataaa taaacttcat aacagacagt accgccggaa tattgttctg catattcgga
    11281 acttccacat agcttacagc gccgcctggt gacagcttct ggaaatcaga ctcaaatttc
    11341 agcttgctga aggcatcgat cttctctgat acatgcacat ggtagctgtt ggtgatatag
    11401 tttttatccg ttactcccgg gataatgcca aaacgcttct gcaggcattt tgcaaacttg
    11461 taagttgtgg actccatagg tgttccatat acggaataac tgatgttctc agccgctttc
    11521 cactctttgc acttatcatt taaacgctgc attaccttta atgcaaatgg acgtgcctcc
    11581 gggtctgtat gggactttcc aagcattctc atgcacattt catacagtcc tgcatagccc
    11641 agtgaaatag tagaatatcc gttaaacaga agcttatcga tcttctcacc ctttttcagt
    11701 cttgccaggg caccattctg ccacaggatc ggtgcaacat cagagatcgt gcctaaaagg
    11761 cgctcatgac ggcatctgag agcttcatgg cataattcca ggcgctcgtc taagatctcc
    11821 cagaaacggt ccatatcgcc ttcagaagaa catgctacat ctaccaggtt aatggtaaca
    11881 acgccctgat tgaaacggcc ataatattta tggctgccat ctgcatttct ctgagagtct
    11941 tctactgtca ggaaagaacg gcatcccata catgggtaaa catctccctg ctttaattcc
    12001 ttcatgatct ttgcagaaat ataatccggt accatacgtt ttgcagtaca ttttgctgcc
    12061 agctctgtca gataccagta tggagcatcc tcggaaatat tgtcctcatc cagcacatag
    12121 atcagttttg ggaacgccgg tgtgatccat acgccttttt cattctttac accctgcata
    12181 cgctgtacca ggacctcttc aatgatcata gacaggtctt ttctggtctg accctctggt
    12241 acctcatcca ggtacataaa tacagttaca aatggagcct ggccattgca ggtcatcaat
    12301 gtgatcagct ggtactgaat agtctggata ccgctcttga tctcttctct cagtctgcgc
    12361 tctgttacct tatcaatgat cgcctcatcc ataggttcgc cgcattcttt tctttcttcg
    12421 atcacatttt tacggatctt ctttctgctg atatctacaa atggagccag atgagccagt
    12481 gtaaatgact gtccgccata ctggttgctg gcaacctggg caacgatctg ggtggtcaca
    12541 ttacaggcag taaaaaaact gtgtggcttc tcgatcagtg tctcactgat cacagtacca
    12601 ttctgaagca tgtcctccaa attgatcaga tcacagttat gttctctctg tgcaaaataa
    12661 tcggtatcat ggaaatggat aattccctct tcatgtgctc ttacgatctt ctccggaaga
    12721 agtacacggc gggaaagatc cttacttact tctccagcca tgtaatcacg ctgggtagaa
    12781 ttgatcaccg ggttcttatt ggaattttcc tggttgacct cttcgttgat attatcaagc
    12841 agtgataaaa ttccattatc tgttgtattg gatttacgtg tcagttccct cttataacga
    12901 tagcgcacat atttctgtgc tacctcgtag cctctcatag ccatgatgcc tgtttccacc
    12961 atgtcctgaa tgtcttctac atttacagca tgagttgact cctgaacctt atttgctata
    13021 ttatctgcta tcgctctgat ctgatagtca ttcatctggt gcagtctgtc tacttcttca
    13081 ttcgccttat gaactgcatt aacgatcttc tgtgaattaa aatgaacctc ttcgccattc
    13141 cttttaatta cattcgtctt tacttcaaca ctcattacta tccctcactc atgcacaaaa
    13201 tattgttgtt tttcaaacca cactcactat atctagtgcc atgtattata atactacaaa
    13261 aatcaccgct tgaaaagggg aacatgtgaa aaagaaagaa ttcagacaat ggcatttttt
    13321 tgccaccatt ttggtaattt gtttattaga aacgcttata cgatatgtat gttttattat
    13381 cggtccctac cggaccgctc agctattatc ccactaactt cctggaatgt aaagcactat
    13441 tttaagaaat attttaaaag atttcgggca taaaaaaaca gctgtaaaat gctttccata
    13501 cttttacaac tgcattcatt tactgcgggt aacaggactt gaacctgcac gtcgtggaca
    13561 ccagaaccta aatctggcgc gtctgccaat tccgccatac ccgcataatt tagaaaaaac
    13621 cgctccctta taactaagag atcggttttc attttaaatg cgggtggccg gacttgaacc
    13681 ggcacggatg ataagtccga gggattttaa gtcccttgtg tctgcctatt ccaccacacc
    13741 cgcatataac aaatggggcc tacagggctc gaacctgtga ccctctgctt gtaaggcaga
    13801 tgctctccca gctgagctaa gaccccaact tgtataaatt gtcttgcata taaatgcaaa
    13861 cgacccggaa cgggatcgaa ccgtcgacct ccgccgtgac agggcggcgc tctaaccagc
    13921 tgagccaccg ggccatgttt gatatagttc ttataacctc agtttaggaa gagtggacct
    13981 tcggggactt gaacccagga ccgaccggtt atgagccggt tgctctaacc aactgagcta
    14041 aaggtccgaa ctatattcgt acctgctgct aagcagtaaa gccgatgatc ggactcgaac
    14101 cgataacctg ctgattacaa atcagctgct ctgccaattg agccacatcg gcatgatgtg
    14161 tgaaccttag ttcaaatgac cccaacgaga ttcgaactcg tgttaccgcc gtgaaagggc
    14221 gatgtcttaa ccgcttgacc atggggccat attttatagt ggtcgccatg acttccacta
    14281 aactccccga gtaggactcg aacctacgac ccaacggtta acagccgttt gctctaccga
    14341 ctgagctatc gaggaatatc aattgcgctg actggtatag tatactatac ttttttgaat
    14401 tttgcaagca caatttttat tttttttgat acataccttc aaaaccacat attgactaat
    14461 gatcatcatt ccgaatactt tacaaccttt gttatccaac tctgcggtca ttcagacctt
    14521 attggtcaag ccctcgaccg attagtatca gtcagctcca tgtgttacca cacttccacc
    14581 tctgacctat ctacctcgtc gtcttcaagg ggtcttacta cttgcgtatg agatatctca
    14641 tcttgagggg ggcttcacgc ttagatgcct t
//
LOCUS       DBMDUA010000057        14469 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2278_length_14469_cov_3.089992, whole genome shotgun sequence.
ACCESSION   DBMDUA010000057 DBMDUA010000000
VERSION     DBMDUA010000057.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 14469)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14469)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14469
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2278_length_14469_cov_3.089992"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="ACZPCL_06165"
     CDS             complement(<1..264)
                     /locus_tag="ACZPCL_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylate symporter family
                     transporter"
                     /protein_id="HIR1162086.1"
                     /translation="MNPIKKWNQLGLFTRIMVGFVLGIAAGLIFGEKATKLAFLGTIL
                     TRLLTMVVAPLVLGLLICSAADVKDFKTLGKIGGKTLGIFLGGT"
     gene            complement(284..1792)
                     /locus_tag="ACZPCL_06170"
     CDS             complement(284..1792)
                     /locus_tag="ACZPCL_06170"
                     /inference="COORDINATES: protein motif:HMM:NF045169.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR1162087.1"
                     /translation="MKVDALLMDPKDNVVTCVRDVKAGEVVHYRSKNEDLEILAKEAI
                     PFSHKISLTDLEEGQEVIKYGELIGKTTRKINAGCLVDHNNIYSVPRDYESELVEEKG
                     EEPVEIKKSEKGEKIQFLGYRRSDGRVGIRNHVLILPACACGSESSRIVASQVRGAVN
                     IVFNTGCSDVADNTAMSQKILTGFACNPNVYGVVIIGLGCETVGHKQLREKIQKMTDK
                     PVVSFGIQEEGGTLKTIEKATRAARELAAQAGLVRKEWCDISELIMGIECGGSDATSG
                     IASNPAVGELSDLLVDLGASTIMSESIEWIGGEHIVAKRGATPKIHNQVIRVCEEYEK
                     HLKQAGQDCRAGQPTPGNKAGGLSTLDEKSLGCIRKGGTRPIVEVLEQAVRPSKHGAI
                     VMDTAGYDISSVTSMVAAGCACVIFTTGRGTPTGNAIAPVLKVTANKRTYTHMEDNMD
                     VDLSGIIEGTKTIEESGKMLLDEIVQVCNGKLTKAEAYGFSDIAVDHVCRFV"
     gene            1929..2879
                     /locus_tag="ACZPCL_06175"
     CDS             1929..2879
                     /locus_tag="ACZPCL_06175"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1162088.1"
                     /translation="MQPEMKYIYTVYQCGSFSKAAEKLFLTQPALSISVQKVEHEVGM
                     PLFNRDKKPLELTEAGMIYIQKIEQIQHLEQELAMQLNDLTDLKTGHLRVGGTHYFNA
                     YILPPVIAAYKKKFPGISLQLIEAGSWELIDMLKNDKIDLTFNCTPDPSDKLRRSPSF
                     QDTILLAVPSHFSVNRFFTKTALSSQDVLARKFEDPDCPSITLQAFTDTPFILLTPGN
                     NLRKRSMTFFKEAGITPNITMEVSQLVTSYHLARSGIGAAFISDWMLTNAHPEMSYYK
                     INSPASARLFDIVTSGKNYLSKAQKAFIDLFQAYYKNYIY"
     gene            complement(2873..5488)
                     /locus_tag="ACZPCL_06180"
     CDS             complement(2873..5488)
                     /locus_tag="ACZPCL_06180"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1162089.1"
                     /translation="MDTNLKNSDDLEKRNVANENIVKENIVKKSHNWKILGIIAMILT
                     VFIAAGTTVGLYPYMEEKAAEFSSRNRRQETEAAGGFGNLATQVMNFSYVLWHQQKQE
                     ENGGILTYSQTFLAGDGIGAVDVLHGEDAQNVPETEKKTDPVSGADAVVSADVAENVV
                     ATESADQTSGQTVAQTEALAEAADPAITEQQQAAGNEDAEDITAFREEIKDLIDELGT
                     TWNNLYNQYNERISYGVMDPQGGYLRSNVNSPEEIFSRTVADNELQFTVHFDENGRLT
                     VSDVTGLESNCSGLIRSIGRFEFYDPLAARLTNSYHRSGLEFSGPRNMTVKFRCQPTK
                     FGSWNEEKPQNVYTYDNYDYQRGHGFITVIMYVSLAVLVAALLIPFIPGMEVVKMKIF
                     SWSLEPLCVIGVGWLLLFLNMEIPLDIVRSFMAGTLQAELAKAGFLPFMANALAVAAN
                     FLFWVVIYGMFYWGVTNFRSFFSLGPWRYFKERTWVGKFLCFCKHWVCTGLDLFSQTD
                     WESRSSKIIGKAVLANFVILACISLLWFWGIGALILYSIALFFLLKKYWGKVQEKYHR
                     LLNCIQEMAEGNLDVKEEEDLGIFEPFKNQLFKVQEGFKKAVEKEVKSERTKTELITN
                     VSHDLKTPLTAIITYVNLLKQENITEEERNSYIQILDQKSMRLKELIEDLFEVSKAAN
                     GTVVLHLEEVDVVSLLKQVRFELSDKIEASGIQFHFDLPNERLAASLDGQKTCRIFEN
                     LLVNITKYGMKGTRAYIKAEKDGEYVQVTLRNISAEELKISPEELTERFVRGDASRNT
                     EGSGLGLAIARSFTEVQGGTMKIEVEGDLFRVILRWKLKDGSEEPEETIQEGSVQEKL
                     VQEEPEADDLDTSDQ"
     gene            complement(5463..6170)
                     /locus_tag="ACZPCL_06185"
     CDS             complement(5463..6170)
                     /locus_tag="ACZPCL_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1162090.1"
                     /translation="MNGDSILLVEDDKEIRQGVEIFLRSQGYQVFQAENGLEGLKILE
                     ERPIDLAIVDVMMPVMDGITMTAKLREKYDFPVIFLSAKAEEVDKILGLNIGADDYVT
                     KPFTPMELMARVNSQLRRYHRFLDRLNSGQKENPKIHQVGGLEVNEETVEVIADGKNV
                     KVTPIEFKILLLLIRNPGRVFPAEEIYERVWNERAINTDTVMVHIRNLREKIEVDPKD
                     PKYIKVVWGVGYKLEKQ"
     gene            complement(6483..7574)
                     /locus_tag="ACZPCL_06190"
     CDS             complement(6483..7574)
                     /locus_tag="ACZPCL_06190"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR1162091.1"
                     /translation="MIDMNVLRDLTNAFGPSGFEEDVVRAVRKHCTGMSLTNDAMNNV
                     YARIGGNAEGRKPVLMLDAHTDECGFMVQGIMENGLLSLIMLGGMDLPSIPAHTLLVR
                     TRAGKLVKGIITSRPVHFMTDKEKSAPLDIREMFLDVGACSKKEAMESFGINIGDPAA
                     PEVAFEYDEEHGLCFGKAFDNRMGCACIIHTLRALQAVKDDLAVEVVGAFASQEEVGM
                     RGATVTSAQVQPDLAIVFEGSPADDFYYSPEKSQGGMHKGVQIRCMDKSYITNPAFLE
                     YAHELGDTMGITYQDTVRRGGSTNAGKISLAGKAVPTLVLGIPSRYVHSHYNFCAIED
                     VENTVRMAVEVIKGLNDERIRHLMHQDIL"
     gene            complement(7567..8733)
                     /gene="iadA"
                     /locus_tag="ACZPCL_06195"
     CDS             complement(7567..8733)
                     /gene="iadA"
                     /locus_tag="ACZPCL_06195"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HIR1162092.1"
                     /translation="MILLKQADVYAPEHLGIKDVLVCGGKIEAVGDNLEGGTGCEVID
                     AKGMRLTPGLIDRHVHVTGGGGEGSFHTRTPQVELSSILKAGITTVLGLLGTDDMSRS
                     VENLLAKVKALKEEGITAYALCGAYGYPPVTLTGSVKKDIAFVDEIMGLKLALSDHRA
                     PNISVDELIRLGSDVRTAGMIGGKPGFIVLHMGSGKKKLGPVFEALAETDIPVKTFQP
                     THMSRNHELYLDGLKLAKMGGYIDITCEDFVNGEPVIGHDPMPALLEEAKAADVPMDH
                     ITFSSDGQGSWSSYDEAGMLKEIGVTDVGNMYAQMCSLVTRGGFDFAEALTYFTSNVA
                     KALEIEKKKGHIMAGADADILLIKDDLTLDTVIAGGRKMMEGGTLLVRGTYEHD"
     gene            complement(8971..10200)
                     /locus_tag="ACZPCL_06200"
     CDS             complement(8971..10200)
                     /locus_tag="ACZPCL_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR1162093.1"
                     /translation="MTEIKGISVDEWKKKICEETKRLRPQLIQLSHSIHDEPEIGFQE
                     FKSAAKICDFLEKQGFETERGYCDLPTSFKAVKKGTGKGPVVAFLAEYDALRGVGHGC
                     GHNVIATCASGAFASLAKIFVEMGIAGEVWIIGTPAEEGGAGKAIMLERGGFDGVDFA
                     LMIHPTCGGESRNYINRNGRASGSLTISFTGQSAHSSAPATGINALTAAISVFNQIDM
                     LRPLFETEDNVNGVILEGGTASNIIPGFSKSEFCIRAATMKRVDELQNMIISCAKRAE
                     SLTGAKVQIQSEPIYAERYPCLPICEDFKENMARVGVGMCLPDPKLLFGSSDIGNVSI
                     KIPAIHDYLSITDEEIPAHSSQYAKAAAEPGADEICIKGAKGLAMTGLDLLLNESLRQ
                     AAAEYHEKVVPDFYKEK"
     gene            complement(10286..11290)
                     /locus_tag="ACZPCL_06205"
     CDS             complement(10286..11290)
                     /locus_tag="ACZPCL_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1162094.1"
                     /translation="METSKTLIEVKNVCQEFHLTKGILQSLRFEKGKLVKEDKVVHAV
                     NDVSFEIKKGEVFSLVGESGCGKSTTARTVIRLLEPTSGQVLYKGKDISHLGPNELLP
                     YRKSMQMIFQDPYASLNPRQRVADIIMEPLLFHGIAKTKEEARERCMNILARVGLRPE
                     QAGRYPHQFSGGQRQRIGIARALAVNPEFIIADEPVSALDVSIQAQILNLLMDLKDEF
                     NFSYLFIAHDLSVVRHISDRLGVMYLGSIVEMGDKHTLFTNPVHPYTKVLFAAVPTVG
                     EKPLVQGIDAKGEIPSPVNLPKGCYFSDRCPYATDRCRQEKPVLREVEPGHMAACHLL
                     "
     gene            complement(11291..12289)
                     /locus_tag="ACZPCL_06210"
     CDS             complement(11291..12289)
                     /locus_tag="ACZPCL_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1162095.1"
                     /translation="MEDKVLLDVQGLKTYFHTFKGVVKAVDNVSFSLKEGEILGIVGE
                     SGSGKSVTSFSILKLVEDPGVVDADHIMFDGKDLAKISERDMSKIRGKDISMVFQDPM
                     TSLNPLYTIQRQMEEVLILHEPQMNKAQRRERCIELLHAVGIPNPEERLKEYPNQFSG
                     GMRQRVIIAIALATNPKLLIADEPTTALDVTIQEQILKLMKKLVKEHNTSLMLITHDL
                     AVVSQMVDKINVMYCGKLVETGKTEDIIYHNAHPYTEGLLNSIPKLYEDRDRLDYIPG
                     MVPNMFELPEGCYFAPRCKYCQEICRHEKPQMQEVGPGHMAACHFPLLKKKEQEEV"
     gene            complement(12300..13208)
                     /locus_tag="ACZPCL_06215"
     CDS             complement(12300..13208)
                     /locus_tag="ACZPCL_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1162096.1"
                     /translation="MEEKVTIEKTGWWKNFLKSEFFYHYKHNIPAIIGSILVVVAILV
                     AVFGRFLAPQNPYDLTAISLADSYLPPVWLDGGKAGFFLGSDEQGRDILSAIIYGSAS
                     SIMIGLVGMICSCAIGTTLGLLAGYFGGKVDAVIMRIADVQLSFPSMLIALFIMSVFG
                     RGVGKLLIALTMVGWVTYARTVRGETLSVKKMEYVEAARTIGLPNRIIIIKHVLPNVI
                     NSIIVLATIQIGSFILTEATLSFLGVGVPITQPSLGLLVKTGFDVLFSGLWWASVFPG
                     LYIMLIVFGINLLGDFLRDELNPNLK"
     gene            complement(13214..14179)
                     /locus_tag="ACZPCL_06220"
     CDS             complement(13214..14179)
                     /locus_tag="ACZPCL_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1162097.1"
                     /translation="MIKYVVKRFAQMIVVLFIVSLLVFLLTNFIGDPVDMLVPENATL
                     EQIESARTRLGLDKPLPVQYGIFIRDVLHGNFGKSYIYGKPAMDLIFERMPATLELVA
                     VAACLVLCIAIPLGVYAGAYPKRASSKLIMSGSILGISLPSFWVGMMMIYIFAVVLRL
                     LPASGRGNTVMVGNVALSIFAPGGIRYVILPAITLALTNVATMLRLTRSGILENMRQD
                     YIKFARAKGVPAKSILFGHALKNALIPVVTIFGMDLGNMIAFTTITETIFAWPGMGKL
                     LIDAINKSDRPIIVAYLMAAACMFVVLNFIVDILYTLIDPRIELR"
     gene            complement(14235..>14469)
                     /locus_tag="ACZPCL_06225"
     CDS             complement(14235..>14469)
                     /locus_tag="ACZPCL_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162098.1"
                     /translation="AFAADFPPVAPEFYQRTHRVRLKNLGTTDKKSSHKIKYKKIPRV
                     HQNMLPESAGAPTSSLYLRVQLTVMIQTSRKGR"
BASE COUNT         3744 a         3608 c         2823 g         4294 t
ORIGIN      
        1 agtaccgcca agaaaaattc ctaatgtctt tcctccaatt tttcctaatg ttttaaaatc
       61 ctttacatct gctgccgaac atatcaaaag tcccagcacc aatggtgcaa ctaccatagt
      121 cagcagacgg gtcaatatag ttccaagaaa tgccagcttt gttgctttct ctccaaaaat
      181 aagtcctgct gcaataccaa gaacaaatcc caccataatt ctggtgaata atcctaactg
      241 gttccatttt tttattggat tcatatgtat gtccccccgt tttttataca aatctgcata
      301 catggtctac cgcaatatca gaaaatccat acgcctctgc ctttgtcagt tttccgttgc
      361 agacctgaac aatctcatct aaaagcattt ttccgctttc ttcaattgtc ttcgtaccct
      421 caatgatccc gcttaaatcc acatccatgt tatcttccat gtgcgtataa gtccgtttat
      481 tggctgttac ttttaaaact ggcgcaatag catttcctgt tggtgttccg cgtccggttg
      541 taaatatgac gcaggcacat ccggctgcta ccatggatgt aacagaagag atgtcatatc
      601 ctgctgtatc cataacaatg gcaccatgtt tgctcgggcg taccgcctgc tccaaaactt
      661 caacgatcgg ccttgttccg cctttacgga tacatccaag actcttttca tccagtgtag
      721 aaaggcctcc tgctttgttt ccaggtgttg gctgtcctgc gcggcagtcc tgtcctgcct
      781 gttttaaatg tttctcatat tcttcacaga cacggatcac ctgattgtgg atcttaggtg
      841 ttgctcctcg ttttgccaca atatgttctc caccgatcca ttcaatagac tcactcatga
      901 tcgtagatgc accaagatcc actaaaagat cacttaactc tcctactgcc ggattggaag
      961 caattccaga tgtagcatct gatccgccac attcaattcc cataatcagt tcagaaatat
     1021 cgcaccattc ttttcttacg agaccagcct gtgctgccag ttctcttgct gctcttgtag
     1081 ccttttcaat ggtctttaag gtaccgcctt cttcctggat tccgaaagac actactggct
     1141 tatctgtcat cttctggatc ttttctctta actgcttatg tcctaccgtt tcacatccaa
     1201 gaccgatgat cacgactccg tatacatttg ggttacaggc aaaaccagtt aatatcttct
     1261 gagacatggc tgtattatct gccacatctg aacatcccgt attaaataca atatttacag
     1321 cccctcttac ctggcttgca acaatacggc ttgactcact tccgcaggca caggctggaa
     1381 ggatcagtac atggttgcgg atccccactc ttccgtcaga acggcggtat cccagaaact
     1441 ggatcttttc tcccttttct gattttttaa tctcaacagg ctcttctccc ttttcttcta
     1501 ccagttcgct ttcatagtca cggggcacgc tgtaaatatt gttatgatct accagacatc
     1561 ctgcattgat ctttcttgtg gtctttccga tcagctctcc atattttata acttcctgtc
     1621 cttcctccag atcagtcagt gagatcttat gggaaaatgg gattgcctct tttgctaaaa
     1681 tttccagatc ctcatttttg ctgcggtaat ggaccacttc ccctgctttt acatctctta
     1741 cacaggttac tacattgtct tttggatcca tgagcagtgc atctaccttc atattctttc
     1801 ctcctgctac attcattttt ttggctcatc tgtatctctt gacattagta tagatctgct
     1861 atatgataaa atcaatttta tggtttttat gttattataa attgaattta tacacaaaag
     1921 gagattccat gcagccggaa atgaaatata tttacactgt ttatcaatgc ggaagctttt
     1981 caaaagcagc cgaaaagctt ttcctaaccc agcctgcttt aagcatttcc gttcaaaaag
     2041 tagaacatga agtaggaatg cccttattca accgggacaa aaagcccttg gaactaactg
     2101 aagccggcat gatctatatt caaaaaattg aacagatcca gcatttagag caggagcttg
     2161 ccatgcagct gaatgacctg actgatctga aaacaggaca tctgcgtgtt ggcggaactc
     2221 attattttaa tgcctatatt cttccccctg ttattgcagc ttacaaaaaa aagttcccgg
     2281 ggatcagtct tcaacttatt gaagctggtt cctgggaact gattgatatg ttaaaaaatg
     2341 acaaaataga cttaaccttt aattgtactc ctgatccttc tgataaactg cgtcgttctc
     2401 cttcctttca ggatacgatc ctgttagctg ttccaagcca tttttctgta aaccgttttt
     2461 tcactaaaac agccctttct tctcaggatg tcctggcacg taaatttgag gatccggact
     2521 gcccttctat tactttacaa gcgtttacag atacaccttt tattttgctt actccaggca
     2581 acaatcttcg taaacggagt atgacttttt ttaaagaagc aggaataact cccaatatca
     2641 ccatggaggt atcacagttg gtaacctctt atcatctggc cagatccggg atcggggcag
     2701 cttttatcag tgactggatg ctgactaacg cacacccaga aatgtcttat tataagatca
     2761 attctcctgc ttctgcgcgg ttatttgata tcgttacttc aggtaagaat tatttatcca
     2821 aagcccaaaa ggcctttatt gatctttttc aggcttatta taaaaattat atctattgat
     2881 ctgacgtatc cagatcatct gcttctggtt cttcctgaac taacttttcc tggactgatc
     2941 cttcctggat tgtttcttcc ggctcttcac tgccatcctt taacttccag cgaaggatca
     3001 ccctgaacag gtctccttct acctctatct tcatggtacc tccctggact tcggtaaagc
     3061 ttcttgcaat ggcaagacca agaccggagc cttctgtatt tctggacgca tcccctctta
     3121 cgaaacgttc tgtcagctct tccggtgaga tcttcagttc ttctgcggag atgtttcgca
     3181 gtgttacctg cacatattct ccgtcttttt ctgccttaat ataagccctg gtgcctttca
     3241 tgccatactt ggtgatgttc accaggagat tttcaaagat acgacaggtc ttctggccgt
     3301 ctaaggacgc cgccaggcgc tcatttggca ggtcaaaatg gaactgaatg ccgctggctt
     3361 ctattttatc ggaaagttca aaacggacct gttttaacag actgaccaca tctacctctt
     3421 ctagatgaag gactacggta ccgtttgccg ccttgcttac ctcaaacagg tcttcgatca
     3481 gctcttttaa gcgcatggat ttctggtcta agatctggat gtaggagttt cgttcttctt
     3541 cggtaatatt ttcctgcttt aacagattta cataagtaat aatggcagtc aatggtgttt
     3601 ttaagtcgtg ggaaacattg gtgatcagct cggttttggt gcgctcgctt ttcacttctt
     3661 tttccactgc ttttttgaag ccttcctgca ccttaaacag ctggttttta aatggctcaa
     3721 aaatccctaa atcttcctct tcttttacat ccagattgcc ttctgccatt tcctggatgc
     3781 agttaaggag tctgtggtat ttttcctgca ccttgcccca gtatttcttc agcagaaaga
     3841 ataacgcaat agagtaaagg atcagcgcgc cgatgcccca gaaccacagc agggagatgc
     3901 atgcaaggat cacaaagttt gcaagcactg cttttccgat gatcttgctg gacctgcttt
     3961 cccagtctgt ctgggagaac agatccagac ctgtgcatac ccagtgtttg cagaagcaaa
     4021 ggaattttcc aacccaggtg cgctctttaa agtagcgcca tggtcctaaa ctgaagaagg
     4081 aacggaaatt agtaactccc cagtaaaaca ttccgtaaat gaccacccag aacaggaaat
     4141 tggctgcaac ggccagagca ttggccataa atggtaaaaa gcctgctttt gccagctctg
     4201 cctggagagt tcctgccata aagctgcgta ctatatctaa cgggatctcc atatttaaaa
     4261 aaagcagcag ccagcccact ccgatgacgc aaagaggctc aagtgaccag ctgaatatct
     4321 tcattttgac cacttccatt cccgggataa agggaatgag aagtgctgct accaggaccg
     4381 ccaggcttac atacataata acggtaataa atccatgccc tctttgatag tcataattat
     4441 catatgtata tacattttgt ggcttttctt cattccagct gccaaatttc gttggctgac
     4501 agcggaattt gactgtcatg ttccttgggc cgctaaattc cagaccagat ctatggtagc
     4561 tgtttgtaag tcttgcagcc aatggatcat agaattcaaa acggccaatg gaacggatca
     4621 ggccggagca gttgctttca agacctgtta catcggacac agtcagcctc ccattttcat
     4681 caaaatgtac ggtaaactga agctcattgt ccgctactgt ccgggagaat atttcctcag
     4741 gactatttac attgctgcgc aggtatccgc cctgtggatc catgacccca taggagatgc
     4801 gttcattata ctggttataa agattattcc aggtggttcc cagttcatcg atcagatcct
     4861 ttatttcttc tctgaaagcc gtaatatctt ctgcgtcttc atttccagct gcctgctgct
     4921 gttctgtaat ggccgggtct gctgcctctg ctaaggcttc tgtctgagcc accgtctgcc
     4981 cggatgtttg atctgcactt tctgttgcaa ccacattttc tgccacatct gcactgacca
     5041 ctgcatctgc accagatacc ggatccgtct ttttttctgt ttccggtaca ttctgtgcat
     5101 cttctccatg cagaacatcc acagccccga ttccatctcc tgcaagaaat gtctgggaat
     5161 aagtaaggat accaccattt tcttcctgtt tctgctgatg ccagagaacg tagctgaagt
     5221 tcatcacctg ggtagccaga tttccaaatc cccctgctgc ttctgtttcc tgtctgcggt
     5281 ttctgctgct gaactccgcc gctttctctt ccatataggg atatagcccc actgtggtac
     5341 ccgcagcaat aaaaaccgtc agtatcatgg ctataattcc cagaattttc caattatggc
     5401 ttttctttac aatattttcc tttacgatat tttcatttgc aacatttctc ttttccagat
     5461 catcactgtt tttcaagttt gtatccaacg ccccacacca cctttatata ttttggatct
     5521 tttggatcca cttcgatttt ttcccggagg ttgcgtatat gtaccatgac agtatctgta
     5581 ttgatcgccc gttcgttcca gactctttcg taaatttctt ctgccggaaa gactcttcct
     5641 ggattgcgga tgagaaggag aaggatctta aattcaatgg gagtcacttt tacgtttttg
     5701 ccgtctgcaa tgacttctac tgtctcctca tttacttcca gtccgcccac ctggtggatc
     5761 tttggatttt ccttctgtcc ggaatttagc cggtctaaga agcggtgata tctgcgcagc
     5821 tgggaattga ctcttgccat taattccata ggtgtaaatg gctttgttac atagtcatcc
     5881 gctcctatgt tcaatcccag gatcttatcc acctcttctg cctttgccga aaggaagatc
     5941 accggaaagt cgtatttttc ccgcagcttt gcagtcatgg tgataccgtc cattaccggc
     6001 atcatcacgt ctacgattgc caggtcaata ggtctttctt ccaggatctt taagccttca
     6061 agaccgtttt ctgcctgaaa tacctgatac ccctggctgc gcaggaaaat ttccactccc
     6121 tggcgtattt ctttgtcatc ctctactaaa aggatactgt ctccgttcat atactcattt
     6181 cctccttacg atttatatag tagcaatgaa attggaagag gcgcttcata aaaaatgaaa
     6241 gtttttctaa agaattataa cataggaagg cttctcccac cagagataga aaaaatacca
     6301 gtaaggaaac cgggtagaga agttcccgcc ggcaggcgga aatgccgtgc tggaaaacca
     6361 gacgcaaaaa atcggcagat ctgcacaaaa aaggaccacc cggtgcagcc acacaggcca
     6421 caccagagca gtcctatttt catgttttcc agatttttcc aaaatcctgt ttttagaaac
     6481 tcttacagga tatcctgatg catcagatgc ctgatccgct catcattcag gcccttaata
     6541 acctcaactg ccatgcggac agtgttttcc acatcttcaa tggcacagaa attataatgg
     6601 ctgtgtacat aacggcttgg aatgccaagt accagggttg gtacggcttt tcctgcaagg
     6661 ctgatcttgc ctgcgttagt gctgccgcct cttcttactg tatcctggta ggtaatgccc
     6721 atggtatcgc ccagttcatg tgcatactct aagaatgcag gattggtgat atagctcttg
     6781 tccatgcagc ggatctgtac gcctttatgc atgccgccct gggatttttc agggctgtag
     6841 taaaagtcat ctgctgggga tccttcaaat acaatagcca ggtctggctg gacctgtgca
     6901 gaggtaacgg tcgcacctct catgccaacc tcttcctggg aggcaaaagc gcctactacc
     6961 tctactgcca ggtcatcttt tactgcctgc agtgctctta aagtgtggat gatgcaggca
     7021 cagcccatgc ggttgtcaaa agctttgcca aagcataatc catgttcttc gtcatattca
     7081 aaggcaactt ccggagcagc cggatcacca atattgatac cgaagctttc cattgcctct
     7141 tttttgctgc atgcacctac atctaagaac atttcacgga tatctaatgg ggcgcttttt
     7201 tctttatctg tcataaaatg taccgggcgg gaggttataa tgcctttgac cagctttccg
     7261 gctctggtgc gtaccagtaa ggtgtgtgcc gggatacttg gcagatccat tccacctaac
     7321 ataataaggc ttaacagacc attttccatg atcccctgta ccataaatcc gcactcatct
     7381 gtatgggcat ccagcataag aacaggcttt ctgccctctg cattaccgcc gatccttgcg
     7441 taaacattgt tcatggcatc attagtcaga ctcatgcctg tgcagtgttt tcttacagct
     7501 cttactacgt cttcctcaaa tccactgggg ccaaaagcat tggtcagatc ccttaataca
     7561 ttcatatcaa tcatgttcgt atgttcctct tactaaaaga gttcctcctt ccatcatctt
     7621 acggccgcct gcaataacgg tatccaatgt taagtcatct ttgatcagca ggatatctgc
     7681 gtcagcgcct gccatgatat ggcctttttt cttctcgatc tccagtgctt ttgcaacatt
     7741 ggaagtaaaa taggtcagtg cctcagcaaa gtcaaaacca cctctggtga ctaaagaaca
     7801 catctgtgca tacatatttc ccacatcagt aacaccgatc tcttttaaca tgcctgcttc
     7861 atcatagctg gaccagcttc cctgaccgtc agaactgaag gtaatgtgat ccataggaac
     7921 atctgctgcc tttgcctctt caagaagtgc aggcattgga tcatgtccga tgactggctc
     7981 gccatttaca aagtcttcac aggtaatgtc aatataaccg cccatttttg ccagtttcag
     8041 tccatccaga tacagttcat gattgcggct catatgagta ggctgaaagg tctttaccgg
     8101 gatatcagtc tcagccagtg cctcaaatac aggccctaat ttcttctttc cgcttcccat
     8161 atgtaaaacg ataaatccag gctttccgcc gatcatgcct gcagtacgta catcacttcc
     8221 taaacggatc agctcatcta cagagatatt tggtgccctg tggtcagaaa gagccagttt
     8281 caagcccatg atctcgtcta caaaggcaat atccttcttt acgcttcctg ttaaggtcac
     8341 cggcggatag ccgtaggcac cgcagagagc ataagcagta atgccttctt cctttaatgc
     8401 ctttaccttc gctaacagat tttccacact gcgggacata tcatctgttc caagaagtcc
     8461 taaaacagta gtgataccag cttttaaaat agaagaaagc tcaacctggg gagttcttgt
     8521 atggaagctt ccttctccgc cgccgcctgt tacatgaaca tgacggtcga tcagacctgg
     8581 tgttaagcgc attccttttg catcgatgac ttcacagcca gtacctcctt ccaggttatc
     8641 tcctactgcc tcgatctttc ctccgcacac taaaacatct ttgattccaa ggtgttccgg
     8701 tgcgtataca tcagcttgtt tcaataatat catggttata tttctcctaa atatattatg
     8761 atgtactctc ggaatctttc ctgcacctgc ttttgcggcc gattttccgc cagtcgcacc
     8821 cgaattttat caacgtacgc accgcgtacg cctcaaaaat ttgggcacaa ctgacaaaaa
     8881 atcttctcgc aaaatcaagt acaaaaagat tctgagagta catattatga atttcccaga
     8941 cgcagctgcg tctggaccgg atatccatat ttacttctct ttgtagaaat ccggtacaac
     9001 tttttcatgg tactcagctg ctgcctgacg gagactttcg ttaagcagca gatccagtcc
     9061 ggtcattgcc agcccttttg cccctttaat gcagatctca tctgcccccg gttctgcagc
     9121 agctttggca tactggctgg aatgggccgg gatctcttca tctgtaatag acagataatc
     9181 atggatcgcc gggatcttaa tggacacatt tccaatgtca gaagaaccaa agagcagttt
     9241 cggatcaggc agacacatgc caacgcctac gcgggccata ttttccttaa aatcttcaca
     9301 gatcggcaga catggataac gctctgcata gatcggttct gactggatct gtacctttgc
     9361 acctgtaagg ctttctgccc gctttgcaca gctgatgatc atattctgaa gctcatcaac
     9421 tcgcttcatc gttgctgcac ggatacagaa ctcagatttt gaaaatcccg gtattatatt
     9481 ggaggcagtt ccgccctcta aaataacccc gtttacattg tcctctgttt caaataacgg
     9541 gcgcagcata tcgatctggt taaacacgct gatagctgcc gtcagggcat tgatgccagt
     9601 tgccggtgca gaggaatggg cagactgtcc tgtaaaggaa atggtaagac ttccggaagc
     9661 tcttccgttc cggttaatat agttcctgct ttcaccgcca caggtgggat ggatcatcag
     9721 tgcaaaatcc actccatcaa agccgccccg ttccagcata atagcctttc cggcaccgcc
     9781 ttcttctgca ggagttccaa tgatccaaac ttctcctgca attcccattt ccacaaatat
     9841 tttcgctaaa gaagcaaatg caccggaagc acaggtggcg atcacattat ggccgcagcc
     9901 atgtcctacg cctcttaaag catcatattc tgctaaaaaa gccactaccg ggccttttcc
     9961 tgttcctttt ttcactgcct taaaggatgt tggaaggtca cagtagcctc tttctgtttc
    10021 aaatccctgt ttttccagga aatcacatat cttggcagct gatttaaact cctgaaaacc
    10081 gatctccggc tcatcatgga tgctatggga aagctggatc agctgcggtc ttaaccgctt
    10141 tgtctcctca catatctttt tcttccattc atccacagag attcctttta tttctgtcat
    10201 gatccacacc tccaaaaaat aaaatgtgta tacaaaaagc tgcagacgta tccaaatggc
    10261 gcctgcagcc ttacttaatt acagcttaaa gcagatgaca agcagccata tgtcctggtt
    10321 ctacttcacg cagcaccggc ttttcctgac ggcagcggtc ggtagcatat gggcatcggt
    10381 cgctgaaata acagcccttt ggaaggttta cagggcttgg gatctctccc tttgcatcaa
    10441 ttccctgaac cagcggcttt tcgcctacag taggaactgc tgcaaacagc accttggtat
    10501 acggatgtac cggattggtg aaaagggtgt gtttatctcc catttccaca atacttccca
    10561 gatacataac gcccagtctg tcactgatgt gacgtacaac tgataagtca tgtgcaataa
    10621 acagataaga gaaattaaat tcatctttca gatccattaa caggtttaag atctgggcct
    10681 gaatggatac gtccagagcg gataccggct catcggcaat aatgaactcc ggatttacag
    10741 ctaaggctct tgcaatacca atacgctgtc tctgtccgcc ggagaactga tgagggtatc
    10801 ttccagcctg ctccggtctt aaaccaacac gggctaaaat attcatgcag cgctctcttg
    10861 cttcttcttt ggtctttgca atgccatgga acagaagggg ttccataatg atgtcagcaa
    10921 ctctctgtct cggatttaag gatgcataag gatcctggaa gatcatctgc atgctcttgc
    10981 ggtaaggaag aagctcattt ggtcctaagt ggctgatatc ttttccttta tagagtacct
    11041 gtccggaggt tggctccaga aggcggataa cagttcttgc tgtagtagat ttaccgcagc
    11101 cgctttctcc taccagactg aaaacttccc cttttttaat ctcaaagctt acatcattta
    11161 cagcgtgaac tactttatct tcttttacaa gctttccctt ttcaaaacga agggactgca
    11221 ggatgccttt tgtaaggtgg aactcctgac atacattttt tacttcaatg agagtcttgg
    11281 atgtttccat ttatacttcc tcctgttctt ttttctttaa cagtgggaag tgacatgcag
    11341 ccatatggcc cggtcccact tcctgcatct gtggcttttc atggcggcag atctcctggc
    11401 agtatttaca tcttggtgca aaataacagc cttccggcag ctcaaacata tttggaacca
    11461 taccgggaat atagtccaga cggtctctgt cctcatacag ctttggaatg gaatttaaaa
    11521 gtccctcagt gtatggatgt gcattatgat aaataatatc ctctgttttt ccggtctcta
    11581 ccagttttcc acagtacata acattgatct tgtcaaccat ctgggataca actgccaggt
    11641 catgagtgat caacatcaga gaggtattat gctccttcac cagctttttc atcagcttta
    11701 atatctgttc ctgaatggta acatccaggg cagtagtagg ctcatctgcg atcagcagct
    11761 tcggattagt agccagtgca atggcaatga taacacgctg gcgcatacca ccggaaaact
    11821 ggtttggata ttcttttaag cgttcttccg gatttgggat gcctactgca tggagcagct
    11881 caatacagcg ctctcttctc tgtgccttgt tcatctgtgg ctcatgaagg atcagcactt
    11941 cttccatctg tctctggatg gtataaagag gatttaaaga tgtcatagga tcctggaata
    12001 ccatggaaat gtcttttcca cggatctttg acatatctct ttcactgatc tttgccaggt
    12061 cttttccatc aaacatgata tggtctgcat caaccacacc cggatcttct accagcttta
    12121 agatagagaa actggtaaca gactttccac ttccggactc acctacgata cctaagatct
    12181 caccttcttt taagctgaag cttacattat ctactgcttt taccacgccc ttgaaggtgt
    12241 gaaaatatgt ttttaatccc tgtacgtcta ataatacttt atcttccata ccgcacctcc
    12301 tatttcagat tcgggttcag ctcatctctt aagaagtcac ctaaaagatt gataccgaat
    12361 acgatcagca tgatatacag acctgggaac acagatgccc accacagacc tgaaaacaat
    12421 acatcaaaac cagtttttac aagcagtccc aaagaaggct gtgtgatcgg cacaccaaca
    12481 cctaaaaagc ttaaggttgc ctctgtcagg ataaagcttc cgatctggat agttgctaaa
    12541 acgataatgg aattgataac gtttggaagt acatgtttta taatgataat gcggtttgga
    12601 aggccaatgg tacgtgctgc ttccacatat tccatttttt taacggaaag tgtttcacca
    12661 cggactgttc ttgcataagt tacccagcct accatagtca gggcgatgag cagttttccc
    12721 acgccccgtc caaatacgga catgatgaat aatgcgatca gcatggatgg gaaagacagc
    12781 tgtacatctg caatacgcat gataacagca tctactttgc cgccaaaata tcctgctaac
    12841 agtcccagtg tggtgccaat ggcacaggaa cagatcatac ctaccagacc gatcataatg
    12901 gaacttgcgc taccatagat aatggcactt aaaatatcac ggccctgttc atcacttccc
    12961 agaaaaaatc cggcttttcc gccgtctaac catacaggtg gaagataaga atctgccaga
    13021 ctgatggcag tcaggtcata tgggttctgc ggagccagga aacgtccaaa tactgcaacc
    13081 aggattgcaa ctaccaccag gatacttccg atgatggctg gaatattgtg tttatagtga
    13141 tagaaaaact cagactttaa aaagtttttc caccatcctg ttttttcaat ggttactttc
    13201 tcttccatgt tcatcacctc aattcaattc taggatcaat gagtgtatat aaaatatcaa
    13261 caataaagtt cagcaccaca aacatgcagg ctgctgccat cagataagcc acgataatag
    13321 gtctgtctga tttattaata gcatcaataa gcagcttgcc catgccaggc catgcaaaaa
    13381 tggtctctgt aatggtggtg aacgcgatca tatttcccag atccataccg aaaatagtaa
    13441 caaccgggat cagcgcattc tttaaagcat gtccaaaaag aatggacttg gcaggaacac
    13501 cttttgctct tgcaaactta atataatcct gacgcatatt ttccaggata ccggaacggg
    13561 tcagtcgaag catggtagcc acattggtca gagccagtgt tattgccgga agaatgacat
    13621 aacggatacc tccgggagca aaaatgctta aagctacatt tcccaccatt accgtatttc
    13681 cacggccgga agccggaagc agtcttaaaa caactgcaaa aatgtaaatc atcatcatgc
    13741 caacccagaa ggaaggaaga gaaataccta aaatagaacc ggacataata agcttgctgc
    13801 tggctctctt aggataagca cctgcatata cacctaaagg aatggcaata cacagtacaa
    13861 gacaggctgc aacagcaact aattcaagag tcgcaggcat acgttcaaag atcagatcca
    13921 tagcaggttt tccatatata taagatttac caaaattgcc atgaagcaca tctctgataa
    13981 aaataccata ctgaacggga aggggcttat caagtcccaa tctggttctt gcggattcga
    14041 tctgttcaag ggttgcattt tccggtacca gcatatcaac cgggtcaccg ataaagttgg
    14101 tcagcagaaa tacgagaaga gatactataa aaagtaccac gatcatctgc gcaaaccgtt
    14161 ttacaacata ctttatcata aaatcttacc tcctgttatc atcagagtat ctaatattcc
    14221 ttttatcctc atcttcacct gcctttgcgg ctggtttgga tcatcaccgt cagctgcaca
    14281 cgcaggtaca gagaggatgt tggggctccg gcggattccg ggagcatatt ttgatgtact
    14341 ctcggaatct ttttgtactt gattttgtga gaagattttt tgtcagttgt gcccaaattt
    14401 ttgaggcgta cgcggtgcgt acgttgataa aattcgggtg cgactggcgg aaaatcggcc
    14461 gcaaaagca
//
LOCUS       DBMDUA010000058        14422 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2289_length_14422_cov_3.112492, whole genome shotgun sequence.
ACCESSION   DBMDUA010000058 DBMDUA010000000
VERSION     DBMDUA010000058.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 14422)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14422)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14422
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2289_length_14422_cov_3.112492"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="ACZPCL_06230"
     CDS             <1..221
                     /locus_tag="ACZPCL_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR1162099.1"
                     /translation="IIHHENLYDLVVSLKEKNKLQKAGERPDLEEEYKILLQAANAQE
                     QCYRDDIDYMASSVEKLLAAMEGCVQKQ"
     gene            240..875
                     /locus_tag="ACZPCL_06235"
     CDS             240..875
                     /locus_tag="ACZPCL_06235"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1162100.1"
                     /translation="MESQTLYRVKKEDLPKLEKLLVKCFAHDPLYCKLIPEKETRERL
                     LPELFKCDLTEFIETCEIYSDSEEMNSLLVVSDESEPYNPLTFYLTEAWASLKTDEYL
                     IKEDPSLKTLWNFMRGRDYLNSRWTDQLHQEERLHVIYLAVDPDMQHHGLAAILMEEV
                     IHYAQEHHLMVSLETHNPDNVPMYEHFGFKLFGIVQKHFDLKQYCLIREIQ"
     gene            1044..2129
                     /gene="leuB"
                     /locus_tag="ACZPCL_06240"
     CDS             1044..2129
                     /gene="leuB"
                     /locus_tag="ACZPCL_06240"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR1162101.1"
                     /translation="MNYNIVAIPGDGIGPEIVREAKKVLDKVGEVYGHKFNYTDILMG
                     GCSIDAYGVPLTDEALETARNSDAVLLGAVGGDVGNSRWYDVAPELRPEAGLLKIRKG
                     LNLFANIRPAYLFEQLADACPLKKEIIGNGFDMVIMRELTGGVYFGARSTEEKDGELY
                     AVDSMPYSEHEIRRIAIKGFEIAMKRRKKLVSVDKANVLDTSRLWRKVVNEVAKDYPE
                     VEVSHMLVDNCAMQLVMNPGQFDVVLTENMFGDILSDEASMITGSIGMLSSASLNESK
                     FGLYEPSHGSAPDIAGKDIANPLATILSAAMLLRYSCDLDKEALAVEAAVRQVLADGY
                     RTGDIMSEGCTKVGTTQMGDLVAERIH"
     gene            2209..3873
                     /gene="ilvD"
                     /locus_tag="ACZPCL_06245"
     CDS             2209..3873
                     /gene="ilvD"
                     /locus_tag="ACZPCL_06245"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR1162102.1"
                     /translation="MRSDAVRKGMQQAPHRSLFNALGMTEEEMNKPLVGIVSSYNEIV
                     PGHMNLDKIVEAVKLGVAMGGGTPVVFPAIAVCDGIAMGHVGMKYSLVTRDLIADSTE
                     CMAMAHQFDALVMVPNCDKNVPGLLMAAARLNIPTVFVSGGPMLAGHVKGCKTSLSSM
                     FEAVGAYAAGKMTEEDVKEYENKTCPTCGSCSGMYTANSMNCLTEVLGMGLRGNGTIP
                     AVYSERIKLAKHAGMQVMEMLRRNIRPRDIMTKEAFMNALTMDMALGCSTNSMLHLPA
                     IAHEAGVELNVDIANEISARTPNLCHLAPAGHTYIEDLNEAGGIYAVMNEISKKGLLN
                     LDCMTVTGKTVGENIKDCVNKNPDVIRPIDNPYSQTGGIAILKGNLAPDSGVVKRSAV
                     APEMLKHEGPARVFDCEEDAIAAIKGGRIVDGDVVVIRYEGPKGGPGMREMLNPTSAI
                     AGMGLGSTVALITDGRFSGASRGASIGHVSPEAAVGGPIALVEEGDIISIDIDNHQLN
                     VLVSDEEMAARKAKWQPREPKVTTGYLARYASLVTSGDKGAILQAK"
     gene            3915..5597
                     /gene="ilvB"
                     /locus_tag="ACZPCL_06250"
     CDS             3915..5597
                     /gene="ilvB"
                     /locus_tag="ACZPCL_06250"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR1162103.1"
                     /translation="MSKLTGAEIVIECLKEQGVDTVFGYPGGAILNIYDALYQHQNEI
                     THILTSHEQGASHAADGYARATGKVGVCLATSGPGATNLVTGIATAYMDSVPVVAITC
                     NVANSLLGKDSFQEIDITGVTMPITKYNFIVKDINRLAKVIRRAFVIAQSGRPGPVLV
                     DITKDVTAATTDYEYQEPEKIEPQTDTIREEDMERALEMIRKAQKPFIFVGGGAVISN
                     ASEELRVFAHKIQAPVADSLMGKGAFDGTDELYTGMVGMHGTKTSNYGITEADLLVVV
                     GARFSDRVIGNASKFAKNAKILQIDIDRAEINKNIKVDASIIGDAKTILRRLNTHLDP
                     INHDEWIAHIERMKDMYPLRYDKNVLTGPFIIQTVNEVTDGDAVIVTEVGQHQMWAAQ
                     YYKYRQPRTLLTSGGLGTMGYGLGASIGAKMGCKDKTVINIAGDGCFRMNMNEIATAT
                     RYNIPVIELIINNHVLGMVRQWQTLYYGKRYSQTVLNDSVDYVKIAEAMGAKAYRVTQ
                     KEELAPVLKEAISLNIPVVIDCQIGCDDKVFPMVSPGAPIADAFDDTDLKIN"
     gene            5917..6999
                     /gene="serC"
                     /locus_tag="ACZPCL_06255"
     CDS             5917..6999
                     /gene="serC"
                     /locus_tag="ACZPCL_06255"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR1162104.1"
                     /translation="MSRVYNFSAGPAVLPEEVLNEAAAEMLDYRGTGMSVMEMSHRSK
                     SYETIIEDAESDLRDLLHIPENYKVLFLQGGGSTQFAMVPMNLMKNRVADYIITGQWA
                     KKAHKEASIYGKANAIASSADKTFSYIPDCSDLPVSEDADYVYICENNTIYGTKFWTL
                     PNTKGKLLVADQSSCFLSEPVDVSKYGLIFAGAQKNVGPAGTVIVIIREDLITEDVLE
                     GTPTMLRYKIHADAKSLYNTPPTYGIYMCGKVFKWLKAKGGLEEMKKINEEKAKILYD
                     FLDESKLFKGTVVKKDRSIMNVPFITGNEELDALFVKESKAAGLENLKGHRTVGGMRA
                     SIYNAMPKAGVEKLVEFMADFEKKHL"
     gene            7015..8178
                     /locus_tag="ACZPCL_06260"
     CDS             7015..8178
                     /locus_tag="ACZPCL_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR1162105.1"
                     /translation="MKKIHCLNPIAACGTDLFPADYEMTDNKAEADAFLVRSASMHEM
                     ELPEGLLAVGRAGAGVNNIPLDECAKAGVVVFNTPGANANGVKELVLAGMFLASRDIV
                     GGIKWCQDNAEDENIAKTTEKSKKAFAGWELKGKKLGVIGLGAIGAEVANAATHLGME
                     VYGYDPYISVNAAWRLSRNVKHITNVDTIFQECDYITVHVPLLESTKGMINKEKMDMM
                     KDGVVILNFARDTLVNDDDMAAALEAGKVARYVSDFPNPKVVHMKHVILTPHLGASTR
                     ESEDNCAVMAVQEITDYLENGNIKNSVNYPACDMGVCQAASRIAVLHMNIPNMIGQIT
                     AILAAQGVNISDMTNKSRDKYAYTLLDLEHKPEETTVEKLKAIEGVLRVRVVK"
     gene            8236..9474
                     /locus_tag="ACZPCL_06265"
     CDS             8236..9474
                     /locus_tag="ACZPCL_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HIR1162106.1"
                     /translation="MAVVKPFICIRPAKENAAKVAALPYDVYNRKEACAAVAGNPISF
                     LNIDRAETQFSDDVDTYADCVYEKARELLDTQIAEGVYVTDAGDHYYLYELTMDGRSQ
                     TGIVACSSIDDYVNGVVKKHENTREDKEIDRIRHVDTVNAQTGPIFLAYRQNETLKAI
                     VAEEKAKPALYDFVSDDGIRHRVWKINDPAQTEAIEAAFAAIPATYIADGHHRAASAV
                     KVGLKRRAENPGYTGEEPFNYFLSVLFPDEELMILPYNRVVKDLNGLSREQFFEAVKE
                     KFELEEIGKEPYAPAEKGTFGMYLDNTWYALKVLPQYKNADPVKGLDVSILQDQLLGP
                     VLGIGDPRTDKRIDFIGGIRGLKELERRVSEDMEIAFSMYPTSIEELLAVADAGLLMP
                     PKSTWFEPKLRSGLFIHRLS"
     gene            complement(9522..10592)
                     /locus_tag="ACZPCL_06270"
     CDS             complement(9522..10592)
                     /locus_tag="ACZPCL_06270"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR1162107.1"
                     /translation="MSFSRKRSFRSFVFIGTELFVLVLLCLCLFMKRTDRQAGIFQTS
                     GDFIKWVDFNVTCEALEQAFRYDVDTCQAEVHLNWVDLLAYLGTRYGGDFSRYKKSDM
                     KKVAEQLINGEMTMEELTSDMKYYDYYRQAYGAVLDGMVGTFQAEIPADEVPEMLQQS
                     AQNTDTVWVTKYGLKAFFPLAKNFPYNDFDDFGVSRSYGFKRRHLGHDMMGQVGTPII
                     AVESGYVEALGWNQYGGWRIGIRSFDKKRYYYYAHLRKNYPFQSNLKEGSAVQAGDVI
                     GYMGRTGYSSKENTNNIEESHLHFGLELVFDESQKESDHEIWINCYELVKFLRRNQCE
                     TVKVEGTKEWRRVYQTKDVPTA"
     gene            10796..11974
                     /locus_tag="ACZPCL_06275"
     CDS             10796..11974
                     /locus_tag="ACZPCL_06275"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1162108.1"
                     /translation="MQNFEFYAPTRMIFGKDTHKQVGKLVKEYGFKKVFVHYGGASAK
                     KSGLLDTVLDALKAENIDYVTLGGVQANPTLAMAREGIELGKKEGADFILAVGGGSVI
                     DSAKCIADGIANPDVDVWKFYMKEETPKAALPVGVILTLSATGSEMSASSVITNTELG
                     LKRGFNSPGHRPLFSICNPELTYTVSPFQTGCGAVDIMMHTLERYFSVGEDTPLSDRI
                     AEGVLKTVIPAGKAALENPNDYEARASIMWAGTISHNDLTGLGRSVFMAVHQMEHELS
                     GMDEKIAHGAGLSALWASWARYVYENNVSRFAQYAVNVWNIEMDFEHPEKTALAGIKA
                     TEDYFKSLNMPTSLRELNVDPASFEELAEKCTNHGTRTLAGIKVLDKPDIIEIYKMAY
                     "
     gene            complement(12120..12923)
                     /locus_tag="ACZPCL_06280"
     CDS             complement(12120..12923)
                     /locus_tag="ACZPCL_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1162109.1"
                     /translation="MEELYVFGTGNAQATHCYNTCFAIKDGDEFFMVDAGGGNGILGI
                     LEKMNVDMAHIHHIFVTHEHTDHILGIVWMVRMIATAMKKGKYEGELRIYCHPGLADT
                     ISTLCRLTLQGKFYKMIGEQIFLIPVEDGETVSILDYDVTFFDILSTKAKQFGFTMHL
                     KNGKKFTCAGDEPYNPECKPYVEGSQWLLHEAFCLYGDRERFQPYEKHHSTVKEACEL
                     AESLSIPNLVLWHTEDKNLSHRKELYTAEGKEYYHGNLLIPDDGEIIKL"
     gene            13113..>14422
                     /locus_tag="ACZPCL_06285"
     CDS             13113..>14422
                     /locus_tag="ACZPCL_06285"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HIR1162110.1"
                     /translation="MRDLAYLQLLAREYPSVKEATSEIVNLMAICSLPKGTEYFFSDL
                     HGEYEAFIHLLRSSSGIIREKIKETFGHLIPEEEQLQLANLIYYPERHLPRMMKVGRY
                     TEDWQKITIYRLVKICREVSSKYTRSKVRKKMPKEFAYIIDELLHVDYSDENKKLYYN
                     EIIHSIIDTSIADKFIVALCRLIQNLTIDNLHIIGDIFDRGPRADIIMKELMNFHDVD
                     IQWGNHDISWMGAAAGNLACICNVLRIAISYNSFDVLEDGYGINLRPLSMFAAKVYQD
                     DPCVRFMPKILDENIYDAVDPGLAAKMHKAIAVIQFKVEEAMMQRHPEYEMENRMLLT
                     AVDYKKGTVTIEGKEYPMLDMNFPTIDPRNPLELTRGEKELLRTLCASFRHSEVLQRQ
                     VKFIYSHGGIYKCYNSNLLYHGCIPMTKDGAFDTIMIGGNGYKG"
BASE COUNT         4273 a         2941 c         3516 g         3692 t
ORIGIN      
        1 gtattatcca ccacgaaaac ctgtatgatc tggtagtaag cttaaaggaa aagaataaac
       61 tgcagaaagc aggagaaaga ccggatctgg aagaagagta caagatcctg ctccaggcgg
      121 caaatgccca ggagcagtgc taccgggatg atattgatta tatggcatct tctgtggaga
      181 agcttttagc tgccatggaa ggctgcgttc aaaaacagta aggagaatag acatatagta
      241 tggaaagtca gacgttatac agagtaaaga aagaagacct gccaaaactg gaaaagctgt
      301 tggtgaaatg tttcgcccat gaccctttat actgcaagct gataccggaa aaagagacca
      361 gggagcgact gctgccggag ctttttaagt gtgatcttac ggaatttatt gaaacctgtg
      421 agatctattc tgacagcgag gaaatgaatt ccctgttggt ggtatcggat gagtcagagc
      481 cttataatcc ccttaccttt tatctgacag aagcctgggc cagcttgaaa acagatgaat
      541 atctgatcaa agaggaccca agccttaaga ccttgtggaa ttttatgaga ggccgtgatt
      601 acttaaattc acgctggaca gaccagcttc accaggaaga acgtcttcat gtgatctacc
      661 tggcagtgga tccggacatg cagcatcatg gtctggcggc catcctgatg gaagaagtga
      721 tccattatgc ccaggagcat catcttatgg tttcactgga gactcacaac ccggacaatg
      781 tgcctatgta tgaacatttt ggttttaaac tttttggcat tgtccagaag cattttgatt
      841 taaaacagta ctgcctgatc cgtgagatcc agtagaaata tgatttaaag ttataccggc
      901 aattccaaac atgtatattt tgtcataggg aaaatgacct ggaacagttg aaatgaagcc
      961 ccaaatgaga tatgattgat agaaattaca gttattacat aatgtataac caaaaggaat
     1021 aataaacaga ggggataata attatgaatt ataatattgt tgcaattcca ggagatggta
     1081 ttggtccgga gatcgtaaga gaggctaaaa aggtattaga taaagtagga gaagtttacg
     1141 gacataagtt caattataca gatattttaa tgggcggctg ctccattgat gcatatggtg
     1201 ttccgctgac agatgaagcc ctggaaacag ccagaaattc agatgcagtg ctgttaggag
     1261 ctgtaggcgg tgatgttggc aactccagat ggtatgatgt agcacctgag ttaagaccgg
     1321 aagcaggact tttaaagatc cgcaaaggct tgaatctgtt tgcaaatatt cgtccagcgt
     1381 atctttttga gcagctggca gatgcgtgcc cattaaagaa agagatcatt ggaaacggct
     1441 ttgatatggt cattatgcgt gaactgaccg gcggtgttta cttcggagca agaagcaccg
     1501 aggaaaagga cggagaactg tacgcagtag actccatgcc atacagcgag catgagatcc
     1561 gccgtattgc aatcaaaggt tttgagatcg ctatgaagcg ccgcaaaaag ctggtaagtg
     1621 ttgataaggc aaatgtgtta gacacttccc gtttatggcg taaggttgta aatgaagttg
     1681 caaaggatta tccggaagta gaagtaagcc acatgttagt tgacaactgt gcaatgcagc
     1741 tggttatgaa cccgggacag tttgacgtag ttttgactga aaacatgttt ggtgatatcc
     1801 tttctgatga agcaagtatg atcaccggtt ctattggaat gttatcttca gcaagcttaa
     1861 atgagtccaa gttcggtctg tatgagccaa gccatggttc tgcaccggat attgcaggaa
     1921 aagacattgc aaacccatta gcaactattc tttccgcagc aatgcttctt cgctattcct
     1981 gtgatctgga caaagaggca ctggcagtag aagcagctgt acgtcaggta ctggcagacg
     2041 gataccgcac cggcgatatt atgtcagaag gctgtaccaa ggttggaacc acccagatgg
     2101 gcgatctggt tgcagagcgt atccactaat taagtattca gagaaaaggg catattttcc
     2161 cgaaaagaaa actggaaaat gtgccagaat ttcaggagga taaaaaatat gagaagtgat
     2221 gcagtaagaa aagggatgca gcaggcacct caccgttctc tttttaatgc acttggtatg
     2281 acagaagaag aaatgaacaa gccattagtt ggtattgtaa gttcttataa tgagatcgtt
     2341 ccgggacata tgaacctgga caagatcgta gaagctgtaa aattaggcgt tgccatgggc
     2401 ggcggtactc cggttgtatt cccggctatt gcagtctgtg acggtattgc catgggacat
     2461 gttggcatga aatattccct ggttaccaga gatctgatcg ctgactctac agaatgtatg
     2521 gcaatggctc atcagtttga tgcgttagtc atggttccaa actgtgacaa gaacgttcct
     2581 ggtctgttaa tggcagcagc aagattaaat atcccaactg tatttgtcag cggcggccca
     2641 atgcttgcag gccacgtaaa gggatgtaag accagccttt ccagtatgtt cgaggcagtt
     2701 ggcgcctacg cagcaggaaa gatgaccgaa gaagatgtaa aagaatatga aaataagacc
     2761 tgtccaacct gtggttcctg ttctggtatg tacaccgcaa acagtatgaa ctgcttaaca
     2821 gaggttcttg gtatgggact tcgtggaaac ggaaccattc cagctgttta ctctgagcgt
     2881 atcaagctgg ctaagcatgc aggtatgcag gtaatggaga tgttaagaag aaacatccgt
     2941 ccaagagata ttatgacaaa agaagcattt atgaatgctt taaccatgga tatggcttta
     3001 ggatgttcta ccaacagtat gcttcactta ccggctattg cacatgaggc cggcgttgag
     3061 ttaaacgtag acattgcaaa tgagatcagc gcaagaacac caaacctgtg ccacctggca
     3121 cctgcaggcc acacctacat cgaagatctg aacgaggcag gcggaatcta cgctgttatg
     3181 aatgagatca gcaagaaggg cctgttaaat ctggactgca tgactgttac aggaaagacc
     3241 gttggagaga acatcaagga ctgtgtaaac aagaacccgg atgtcatccg cccaatcgac
     3301 aatccataca gccagaccgg cggtatcgct atcttaaagg gcaacctggc gccagattca
     3361 ggcgttgtaa agcgttctgc agttgctcct gaaatgttaa agcatgaagg accggcaaga
     3421 gtctttgact gtgaggaaga cgctattgca gctatcaagg gcggcaggat cgttgacggt
     3481 gatgttgttg tgatccgtta tgagggacca aagggcggtc ctggtatgag agaaatgtta
     3541 aacccaactt ctgctattgc aggaatggga cttggctcta cggtagcact gatcacagac
     3601 ggacgtttct ccggagcttc cagaggcgct tccattggac atgtttctcc tgaggcagct
     3661 gtaggcggcc cgatcgcttt agtagaagag ggagatatca tttccattga tattgataat
     3721 catcagttaa acgtactggt ttctgatgaa gaaatggcag ccagaaaggc taaatggcag
     3781 ccaagagagc ctaaggtaac caccggttat ctggcaagat atgcttcact ggtaacttcc
     3841 ggtgacaagg gcgctatcct tcaggcaaaa taaggacagc atacaggatt atttcgtaaa
     3901 ggagcacagg acaaatgagt aaattgacag gtgcggaaat cgttatagaa tgtttgaaag
     3961 aacagggggt agacaccgtt ttcggatatc cgggcggtgc catcctgaac atatatgatg
     4021 cgctgtatca gcaccagaat gagatcaccc atatccttac atctcatgaa cagggcgctt
     4081 cccatgcagc agacggatat gccagagcaa ccggaaaggt aggcgtatgc ctggcaactt
     4141 ccggccctgg tgcaaccaac ctggtaacag gtattgctac cgcgtacatg gactccgttc
     4201 cagtagtggc tattacctgt aacgtggcaa acagcctctt aggtaaggac agtttccagg
     4261 agatcgatat cacaggtgtg accatgccga tcaccaagta caatttcatt gtaaaagaca
     4321 tcaacaggct ggcaaaggtg atccgcagag ccttcgtcat cgcccagagc ggaagaccgg
     4381 gccctgttct tgtagatatt accaaggacg taactgcggc taccacggat tatgagtatc
     4441 aggagccgga gaagatcgag cctcagactg atactatcag agaagaagac atggaacgtg
     4501 ctttagagat gatccgcaag gcacagaagc catttatctt cgtaggcggc ggcgctgtta
     4561 tctccaatgc gtctgaagaa ctgcgggtgt ttgcacacaa gatccaggcg ccggttgcag
     4621 acagtttaat gggtaagggc gcatttgacg gtactgacga gctgtataca ggtatggttg
     4681 gtatgcacgg aaccaagact tccaactacg gcattacaga ggcagacctg ttagtagtag
     4741 ttggtgccag atttagtgac cgtgtgatcg gaaatgcatc taaatttgcg aaaaatgcaa
     4801 agatcttaca gatcgatatt gaccgtgctg agatcaacaa aaatatcaag gttgacgcaa
     4861 gtatcattgg agatgccaag accattttac gccggttaaa tacccatctg gatccgatca
     4921 accatgacga atggatcgct catattgagc gtatgaaaga tatgtatcct ctgcgttatg
     4981 acaagaacgt actgaccggt cctttcatta tccagactgt aaatgaagtg accgacgggg
     5041 acgctgtgat cgtaaccgag gttggacagc accagatgtg ggctgcccag tattataaat
     5101 accgtcagcc gagaactctc cttacttccg gcggactggg aaccatggga tacggcctgg
     5161 gcgcttctat tggcgctaaa atgggctgca aggataagac ggtcatcaac attgcaggtg
     5221 acggctgctt ccgtatgaac atgaacgaga ttgctacagc tacccgttac aatatcccgg
     5281 tgattgaact gatcatcaac aaccatgtat taggtatggt gcgtcagtgg cagactctgt
     5341 attacggaaa gcgttattcc cagactgtac ttaacgactc tgttgattat gtaaagatcg
     5401 cagaggcaat gggcgcaaag gcttacagag tgacccagaa ggaagaactg gctcctgtat
     5461 taaaagaggc catctcctta aatatcccgg ttgttatcga ctgccagatc ggctgcgacg
     5521 acaaggtatt cccaatggta tctcctggag caccgatcgc agatgcattt gatgatacag
     5581 atttgaaaat taactaaata attttcaatg atttatttaa aactaaaaaa agtttcagat
     5641 aaatttagct tgaaaaataa aatgaaacgt tgtatagtaa tttctaacac cttggaacgt
     5701 taccatgtta ttggtttata ctgataaagt aagaaaaaag ataggattac ttatacaagg
     5761 gcgtatatac acaggtggct tctctgtgtg atgcaggaaa attttcccat ttgcatcatg
     5821 caggggagcc gcttgcatat atagataaaa gggtgcacaa aaattaaaaa taataagcac
     5881 caaatattcc aagagaattc caagaggagg aaaactatga gcagagttta caacttttca
     5941 gcaggaccgg cagttttacc ggaagaagtc ctgaacgaag cagcagcaga aatgctggac
     6001 tacagaggta caggaatgtc agtaatggag atgagtcatc gatctaagtc atatgagacc
     6061 attattgaag atgctgaatc agatctgcgc gatctcttgc acattccgga aaattataag
     6121 gtgcttttcc ttcagggcgg cggttctacc cagttcgcca tggttcctat gaacctgatg
     6181 aagaatcgtg tggcagatta tatcattacc ggacagtggg caaagaaagc acacaaagag
     6241 gcatctattt acggaaaggc aaatgccatt gcttccagtg ctgacaagac tttcagctat
     6301 attccggatt gctccgatct gcctgtatct gaagatgcag attatgtgta catctgtgaa
     6361 aataatacaa tttacggaac caagttctgg actctcccga atacaaaggg aaaactgtta
     6421 gtagcagatc agtcttcctg cttcttatct gagcctgtgg acgtatccaa atacggcctg
     6481 atctttgcag gcgcacagaa gaacgtagga cctgcaggta ctgttatcgt tatcatccgt
     6541 gaggacctga tcacagaaga cgtattagag ggaaccccaa ctatgttgcg ctacaagatc
     6601 catgcagatg caaaatccct ttacaataca cctcctacct acggtattta catgtgcgga
     6661 aaggtattta agtggttaaa ggcaaaaggc ggcctggaag agatgaagaa gatcaacgag
     6721 gagaaagcaa agatccttta cgacttctta gacgaaagca agctgtttaa gggcactgta
     6781 gtaaagaaag accgttctat catgaacgta ccatttatca ccggaaatga agaactggat
     6841 gccttatttg taaaagagtc aaaggcagca ggtcttgaaa acttaaaggg ccacagaacc
     6901 gtaggcggta tgcgcgcaag tatctacaat gcaatgccaa aggccggtgt agagaagctg
     6961 gttgagttta tggctgactt tgagaagaag catctgtaaa gaggagagag tattatgaaa
     7021 aagatccatt gcttaaatcc aattgcagcc tgcggaacgg acctgttccc ggctgattat
     7081 gaaatgaccg acaacaaagc agaggcagat gcctttttag tacgaagcgc ttccatgcat
     7141 gaaatggagc tgcctgaggg acttctggca gtaggccgtg caggcgcagg tgtaaacaat
     7201 atcccgttag atgagtgcgc aaaggccggt gtagttgtat ttaacacacc aggtgctaat
     7261 gcaaacggcg taaaagagct ggttcttgca ggtatgtttt tagcatcccg tgacattgta
     7321 ggtggtatta aatggtgcca ggacaatgca gaggatgaaa acattgcaaa aacaaccgaa
     7381 aagagcaaaa aggcttttgc aggctgggaa ttaaagggca agaagttagg tgttatcgga
     7441 cttggtgcta ttggtgctga ggttgccaat gcagctaccc atctgggaat ggaagtttac
     7501 ggctacgacc catacatctc cgtcaacgca gcatggagac tttccagaaa tgtaaagcac
     7561 atcaccaacg tggatactat tttccaggag tgcgattata tcaccgttca tgtgccgctg
     7621 ttagagtcta ccaagggcat gatcaacaaa gaaaaaatgg atatgatgaa ggatggcgtg
     7681 gtgatcttaa actttgcaag agatactctt gtaaatgatg atgatatggc cgcagcactg
     7741 gaggctggaa aagttgcccg ttatgtcagc gacttcccaa atccaaaggt agtacatatg
     7801 aaacatgtga tcctgactcc tcatctgggc gcttccacaa gagagtctga ggacaactgt
     7861 gcagtaatgg cagtacagga gatcacagat tatctggaaa atggtaatat caagaattct
     7921 gttaattatc cggcctgcga tatgggcgta tgccaggcag caagccgtat tgccgttctt
     7981 cacatgaaca ttcctaacat gatcggacag atcactgcca tcctggcagc ccagggcgtg
     8041 aacatttctg atatgaccaa taagagccgt gataagtatg cttatacctt attagatctg
     8101 gaacacaagc cggaagagac tactgtagag aaattaaagg ccattgaggg cgttttacgg
     8161 gtacgtgtag tgaaataatg ttacagatgt catgaccgaa ctgtaacaaa taaaggtgat
     8221 cagaaggaga agaaagtggc agtagtaaaa ccatttatat gtatcagacc tgcaaaggag
     8281 aatgcagcta aggttgcagc tcttccttat gacgtataca accggaaaga ggcatgtgcg
     8341 gcagtagcgg gaaatcctat ttctttctta aatattgaca gggcagagac tcagttttcc
     8401 gatgatgtgg atacgtatgc agactgtgta tacgaaaaag caagagagct tttagatacc
     8461 cagatcgcag aaggggtata tgttacagat gcaggagatc attattatct gtatgaactg
     8521 accatggatg gacgcagcca gacaggtatc gttgcctgca gttccattga tgattatgta
     8581 aacggtgtgg tcaaaaagca tgagaacacc agagaagaca aggaaataga ccgtatccgc
     8641 cacgtagata ctgtaaatgc ccagacaggc cctattttcc tggcttacag acagaatgag
     8701 accttgaagg ccatcgtagc agaagagaag gcaaaaccgg ctctctatga ttttgtatct
     8761 gatgacggca tccgccacag agtgtggaag atcaatgatc cggcacagac agaagccatt
     8821 gaggcggcat ttgcggcaat tccggctacc tatattgcag acggacacca cagagcagct
     8881 tccgctgtga aggttggctt aaagcgcagg gcggaaaatc ctggatacac aggagaagag
     8941 ccatttaact acttcctgtc tgttttattc ccagatgagg aactgatgat ccttccatac
     9001 aaccgtgtag tgaaggactt aaacgggctg agtagggagc agttctttga ggctgtaaaa
     9061 gaaaaatttg aactggaaga aattggaaaa gaaccatatg ctccagctga aaaaggaacc
     9121 ttcggcatgt acttagacaa cacctggtat gctttaaagg tacttcctca gtacaagaac
     9181 gctgacccgg taaagggtct ggatgtttcc attttacagg accagctgtt aggaccggta
     9241 cttggcatcg gtgatccaag aaccgacaag cgtatcgact ttatcggcgg catccgcggt
     9301 ttaaaagagc tggaacgccg tgtaagcgaa gatatggaga ttgccttttc catgtatcca
     9361 acttccattg aagagctttt agcagtagca gacgcaggcc ttttaatgcc gcctaagtct
     9421 acctggtttg agccgaagtt aagaagtgga ttatttatcc acagattaag ttaaataagt
     9481 tgatttgagg ccggccgcac gattgtgcgg ctggtctttt tctatgcagt gggaacatct
     9541 ttcgtctgat atacccgcct ccattctttg gtgccctcta cttttacagt ttcacactgg
     9601 ttcctgcgca ggaactttac cagttcgtag cagttgatcc agatctcatg gtcactttct
     9661 ttctgtgact cgtcaaagac cagttctagg ccgaagtgaa ggtgggactc ttcgatattg
     9721 ttggtatttt ctttggaact gtagccggtg cgccccatat atccgatgac atcaccggcc
     9781 tggacagcgc tgccttcttt taaattggac tggaaggggt agtttttgcg caggtgggcg
     9841 tagtaatagt accgcttttt gtcaaagctg cggataccga tccgccagcc cccgtactgg
     9901 ttccagccca gtgcttccac atagccggac tccactgcga taatgggagt tcccacctgc
     9961 cccatcatgt catggcctaa gtggcggcgc ttgaagccat agctgcggga gactccgaaa
    10021 tcatcaaagt cgttgtaagg aaagttcttt gccagtggga agaaggcttt cagtccgtat
    10081 tttgtgaccc agacggtatc agtattttgc gcagattgct gcagcatttc cggcacttca
    10141 tccgccggga tctctgcctg gaatgtccct accattccgt ctaacaccgc cccataagcc
    10201 tgtctgtaat agtcataata tttcatatcg cttgtcagtt cttccatggt catttcacca
    10261 ttgatcagct gttccgccac ctttttcata tcacttttct tatatctgga aaaatccccg
    10321 ccatagcgtg ttccaagata cgccagcagg tccacccaat ttaaatgaac ctctgcctgg
    10381 caggtatcca catcgtaccg gaacgcctgc tccaacgctt cacaggtaac attaaaatcc
    10441 acccatttaa taaaatcacc ggaagtctgg aaaataccag cctgccggtc cgttcttttc
    10501 ataaaaaggc aaagacataa aagaaccagg acaaaaagtt ccgtaccgat aaagacaaat
    10561 gaacgaaatg aacgttttct ggaaaaagac ataatatccc ccttttgttc tggagcaagt
    10621 ttgcttaaga tacctgtacc cataatcata tgctgattat gacagggata tgtttcttac
    10681 aaggggttcc cttgttacaa aaggaaaaat atggtaagat aatgaataga ttggccatgt
    10741 taaatggtcc aagatcaggc agtataaaat caatgatcgt atggaggata aggcaatgca
    10801 gaattttgaa ttttatgcac caacccggat gatttttgga aaagatactc acaagcaggt
    10861 gggaaaactg gtaaaggagt acggttttaa gaaggttttt gttcattacg gcggtgcaag
    10921 cgcgaagaag agcgggcttc ttgatactgt attagatgcc ttaaaggcag aaaatatcga
    10981 ctatgtgacc ttaggcggcg ttcaggcaaa tccgactctg gctatggcaa gagaaggcat
    11041 tgaactgggc aaaaaggaag gcgctgactt tatccttgca gtaggcggcg gaagtgtcat
    11101 cgactctgca aaatgtattg cagacggtat tgcaaacccg gatgtagacg tatggaagtt
    11161 ctatatgaaa gaggaaactc caaaggctgc tctgccggta ggcgttatcc ttacactgtc
    11221 tgcaaccgga agtgaaatga gtgcttccag cgttatcacc aatacagaac tgggcttaaa
    11281 acgaggattt aacagccctg gacacagacc attattctct atctgcaatc cggagctgac
    11341 ctataccgta agtccgttcc agaccggctg cggagctgtg gatattatga tgcatacgtt
    11401 agaacgctat ttcagtgttg gtgaggatac accattatcc gaccgtattg cagagggagt
    11461 tttaaagaca gtgatcccgg ctggaaaagc agcactggaa aatccaaatg attatgaagc
    11521 aagagcgtcc atcatgtggg caggaacaat ttcccacaat gacctgaccg gccttggaag
    11581 atccgttttt atggcagtac atcagatgga gcatgaatta tccggtatgg atgagaaaat
    11641 tgctcacggc gcaggacttt ccgctttatg ggcatcctgg gcaagatatg tatatgaaaa
    11701 taatgtaagc cgctttgccc agtacgcagt gaatgtatgg aacattgaaa tggattttga
    11761 gcatccggaa aagaccgctt tggcaggtat taaggctaca gaagattatt tcaagagcct
    11821 gaatatgcca acctctttaa gagaactgaa tgtggatcct gcatcttttg aagaactggc
    11881 agaaaaatgc accaaccacg gcacaagaac actggcaggt attaaggtat tggacaagcc
    11941 ggatattatt gaaatttata agatggcata ttaaaaagca gcagtaagag agaacgcctg
    12001 ctggcgcagg cggtactccg gcagacatag aaacagcctt cagaaaagga acaaaatgtt
    12061 ccaattctgg aggctgtgct gcgttatcat gggttcataa aaaatatcag tgacatgttt
    12121 tacagtttta taatctcccc gtcatcagga atcagcagat ttccatgata atattctttt
    12181 ccttctgctg tgtaaagttc tttcctgtgg cttaagtttt tatcttctgt atgccacagc
    12241 accagatttg gaatagaaag gctttctgcc agttcgcagg cttcttttac tgtgctgtga
    12301 tgtttttcat aaggctggaa ccgttctctg tcaccataga ggcagaatgc ttcgtgaaga
    12361 agccactgac tgccttccac atatggctta cattccggat tgtaaggctc gtctccggcg
    12421 caggtgaact tctttccgtt tttcaggtgc atggtgaagc cgaactgttt tgcctttgta
    12481 gacaaaatat caaagaaagt tacatcataa tctaaaatag aaacggtttc tccgtcttct
    12541 accgggatca ggaagatctg ttccccgatc attttataaa atttcccctg aagtgttaaa
    12601 cggcataagg tagagatcgt atctgccagt cccggatggc agtagatgcg aagttcccct
    12661 tcgtattttc cttttttcat ggcagtggcg atcatgcgga ccatccagac aattccaagg
    12721 atatggtcgg tgtgctcatg ggtgacaaaa atgtgatgga tatgggccat gtccacattc
    12781 attttttcca gaatacctaa aataccatta ccgccgccgg catctaccat aaaaaattca
    12841 tctccgtctt ttatggcaaa gcaggtattg taacagtggg ttgcctgtgc gtttccggta
    12901 ccaaatacgt ataattcttc catatttcga tcctttcttg tgcatttctt cataaatctt
    12961 aacaaaatgt aagcttgtgt tcctgcggaa aaacttttat tttcaataag aatatggtac
    13021 tataatccca tataaaacgc aagatttcct tgataaagtc ttttttggga catgtaaaga
    13081 aaggaaaatg cgataagatg ggggaatgta atatgaggga tctggcttat ctgcagcttc
    13141 tggcgcgtga atatccgtca gttaaggaag ctaccagtga gatcgtaaac ttaatggcga
    13201 tctgtagtct tccaaaagga acagagtatt ttttcagtga tcttcatgga gagtatgaag
    13261 cgtttatcca tttactccgg tcttcttccg gtatcatccg tgaaaagatc aaggaaacat
    13321 tcggacattt gatcccggag gaggagcagt tacagttagc aaatctgatc tattatccag
    13381 agcgtcatct tccaaggatg atgaaagttg gacggtatac agaagactgg cagaagatca
    13441 ccatttacag actggttaag atctgccgcg aagtttcttc caaatatacc cgttcgaagg
    13501 taagaaaaaa gatgcccaag gagtttgctt atattattga tgagcttctc catgtagact
    13561 acagcgatga gaacaagaaa ctttactaca atgagatcat ccattctatt attgatacca
    13621 gtatagcaga caagtttatt gttgcgctgt gccgtttgat ccaaaatctg accattgata
    13681 atcttcacat tatcggcgat atttttgatc gtggaccaag agcggatatt atcatgaagg
    13741 agttaatgaa cttccatgat gtggacatcc agtggggaaa tcatgatatt tcctggatgg
    13801 gagcagctgc cggcaatctg gcctgtatct gtaatgtgct tcgtatagcc atcagctaca
    13861 acagctttga tgtgctggaa gatggatacg gtatcaatct gcgtccactg tccatgtttg
    13921 cggcaaaagt ttaccaggat gatccatgtg tccgctttat gccaaagatc ctggatgaaa
    13981 atatttatga tgcagtagat cctggcctgg cagctaagat gcacaaggca attgctgtga
    14041 tccagtttaa ggtagaagaa gcgatgatgc agcgccatcc ggagtatgag atggaaaacc
    14101 gcatgctttt aactgcagtg gattacaaga aaggaaccgt taccattgaa ggaaaagaat
    14161 acccaatgct ggatatgaat ttcccaacca ttgacccgcg aaatccactg gagctgacca
    14221 gaggagaaaa ggaactgtta cgcactctct gcgcgtcatt ccgccacagc gaggttttac
    14281 agcgtcaggt gaaatttatc tattctcatg gtggtattta caagtgctac aactccaatc
    14341 tgctgtatca cggatgtatt cctatgacaa aagatggagc atttgatacc attatgatcg
    14401 gtggaaatgg ttataaagga aa
//
LOCUS       DBMDUA010000059        14381 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2296_length_14381_cov_2.614846, whole genome shotgun sequence.
ACCESSION   DBMDUA010000059 DBMDUA010000000
VERSION     DBMDUA010000059.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 14381)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14381)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14381
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2296_length_14381_cov_2.614846"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="ACZPCL_06290"
     CDS             complement(<1..476)
                     /locus_tag="ACZPCL_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1162111.1"
                     /translation="MKYNFDEVIDRSNNRSSKYDERLKKFGTEDVIPLWVADMDFKTA
                     QPIIDACKKKAEEGIWGYTSRPASYFEAVQEWEEKYYGWKPETSHMSWCLGVVQTMAA
                     LMKIYTMPGDKVLIQTPVYSEFYDIPEAIGRVVVENPLVEENGSWHVDYEDFERKV"
     gene            complement(745..2850)
                     /locus_tag="ACZPCL_06295"
     CDS             complement(745..2850)
                     /locus_tag="ACZPCL_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR1162112.1"
                     /translation="MKKIAVVALDPLAGASYTKEVRDLFDEYAEVVGYSVRDGSAAGV
                     LPRADLFAISTDAYGSAEEEARHVPIDSQIMSIEVTFYWETLKKLFEIPQGTKVLFVN
                     VTSNMAREAITQLSSLGVNHLQFIPYYPGAVLEEPVDIAVTPGESRFVPPSVKTVIDC
                     DHRPCSYGMMVEIALRLGLEYLPETESFMNYAKVVASNHYSFDLMYAKSRRQESQMHI
                     LAESLDEGLIGVNETGEVFVCNKKACQIARISEELAMGKKGEEVFPYIPFYQVLREKK
                     AVPEKVIRLFGTNVSLAVVPVVRKEDCIGAFAMLQKFNEQESRQNALRRQLMQKGHYA
                     RYTFDDVIGNSDTITETINILRKMAATESPVLLIGETGTGKELMAHAVHQASGRAAGP
                     FIAINVAAMPENLLESELFGYEEGAFTGAKKGGRPGLFEFAHHGTLFLDEVEGMSLSM
                     QVKLLRVLQEGEIMRVGGGSIIRVDVRIVAATNEALEKKIQEGSFRKDLYYRLNALTV
                     EIPPLRKRGDDIFMLFDFFMRKMGGNFTLSEEVKAFLRRHPWPGNIRELQNAVEYFNY
                     LAKPVIELSDLPPTMTRVVGVGLDVVGPGESSGENENVKTGTTQLKEGSTYSGHMDTE
                     AKKQFVLKQLALSWKAGETVGREKILEAAKKDHIPMTQKQARDLLKELEKEGLLQVGR
                     GRGGSRITEKGLKKLEKQK"
     gene            3037..3897
                     /locus_tag="ACZPCL_06300"
     CDS             3037..3897
                     /locus_tag="ACZPCL_06300"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR1162113.1"
                     /translation="MTFTYKIPCFDLEQTAGSGQVFRMIPAPGRTNTWQVISQGHVCF
                     IRQENDIFFLECGPDLENFWKNYFDLNTDYEKMIASITPDDHYLKAASKAGQGIRILR
                     QDTWEMIITFVISQQKTIPAIRALVEALCSRYGTKIDQFYAFPTPEQLCQASLEDLLA
                     LKLGYRAKYIYRLCRDAVAGRLDLAHLSKLDYTAAMEYLTGFYGIGKKVANCVCLFGL
                     HHIDAFPVDTWIEKILNEHYYDAEKYKDLPKARLYDQMITDSFGCYKGYAGVMQQYIF
                     NYERNVIHGK"
     gene            complement(4009..4572)
                     /locus_tag="ACZPCL_06305"
     CDS             complement(4009..4572)
                     /locus_tag="ACZPCL_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162114.1"
                     /translation="MLIKKRKNTVPPWKIFILTVVGFGLMLAIAVHSRNEALNRLSQE
                     YTITDDAKPRHIKFESMPVGEAEQAVGMYLRYNAMVQFEESGKILSDDLAKNVPFDSM
                     QADFENGIYPQDVLVHGFKTLSEEEYGDEKSQYDNHATLLGYTSYKVVQVSLDEQWPD
                     ETKENITRQYAVGRSRKSWKIFEITEK"
     gene            complement(4588..5730)
                     /gene="dnaJ"
                     /locus_tag="ACZPCL_06310"
     CDS             complement(4588..5730)
                     /gene="dnaJ"
                     /locus_tag="ACZPCL_06310"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR1162115.1"
                     /translation="MAENKRDYYEVLGVSKDADEDTLKKAYRKLAKKYHPDANPGDKE
                     AEAKFKEASEAYSVLSDPQKRQQYDQFGHAAFEQGGGAGYGGGFGGFDFNGGDMGDIF
                     GDIFGDIFGGGRSRSRSGPMKGANVRTAVRITFEEAVFGCEKEIEINYKEVCSNCNGT
                     GAKPGTSPQTCTKCNGKGKIMYTQQSFFGQVQNVQTCPECNGTGKVIKEKCPNCYGTG
                     YKTVRKKFKVSIPAGIDNGQCIRMAGGGEPGTNGGERGDLLVEAVVSQHPIFKRQDTS
                     IYSTVPISFAKAALGGPIRIKTVDGEVEYEVKPGTQTDTKVRLKGKGVPSLRNRNVRG
                     DHYVTLVVKVPERMNQAQKDALKRYDDLMNGIEPEGEKPKKKGLFK"
     gene            complement(5914..7785)
                     /gene="dnaK"
                     /locus_tag="ACZPCL_06315"
     CDS             complement(5914..7785)
                     /gene="dnaK"
                     /locus_tag="ACZPCL_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR1162116.1"
                     /translation="MSKIIGIDLGTTNSCVAVMEGGKPTVIANAEGVRTTPSVVAFTK
                     TGERLVGEPAKRQAVTNADRTIASIKRHMGTDYKVDIDGKKYTPQEISAMILQKLKAD
                     AESYLGETVSEAVITVPAYFNDAQRQATKDAGKIAGLDVKRIINEPTAAALAYGLDNE
                     KEQKIMVYDLGGGTFDVSIIEIGDGVIEVLSTNGDTHLGGDDFDNAITNWMISEFKKA
                     EGVDLSGDKMALQRLKEAAEKAKKELSTATTTNINLPFITATAEGPKHLDMNLTRAKF
                     DELTHDLIERTAIPVQNALKDAGITAAELGKVLLVGGSTRMISAQEKVKQLTGKEPSK
                     SLNPDECVAIGAAIQGGKLAGDAGAGDILLLDVTPLSLSIETMGGVATRLIERNTTIP
                     TKKSQIFSTAADNQTAVDIHVVQGERQFARDNKTLGQFRLDGIPPARRGVPQIEVTFD
                     IDANGIVNVSAKDLGTGKEQHITITSGSNMSDSDIDKAVKEAAEYEAQDKKRKEAIDA
                     RNDADSMVFQTEKALEEVGDKIDANDKAEVEADLNALKEAINRAPVDQMTDAQVEDIK
                     SGKEKLMNSAQKLFAKVYEQAQQAGAGAGPNMGGANAGNAGSSKPDDDVVDADFKEV"
     gene            complement(7926..8474)
                     /gene="grpE"
                     /locus_tag="ACZPCL_06320"
     CDS             complement(7926..8474)
                     /gene="grpE"
                     /locus_tag="ACZPCL_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR1162117.1"
                     /translation="MEQEASEETASENTETSAEEKTTEKAPEKEGFFKKKKDPKDEKI
                     EELTDRVKRQMAEFDNFRKRTEKEKSTMYEMGAKDIIERMLPVVDNFERGLASIPESE
                     KNTAFAEGMEKIYKQFQKVLEDAGVKAIEAAGQPFDPNFHNAVMHVEDESLGENIVAE
                     ELQKGYMYRDSVVRHSMVKVAN"
     gene            complement(8525..9571)
                     /gene="hrcA"
                     /locus_tag="ACZPCL_06325"
     CDS             complement(8525..9571)
                     /gene="hrcA"
                     /locus_tag="ACZPCL_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR1162118.1"
                     /translation="MDAQLDERKVTILKAIIKTYLETGEPVGSRTISKYSELKLSSAT
                     IRNEMSDLEEMGYIIQPHTSAGRIPSDKGYRFYVDQIMQEKENEVTEFKDVMVQKVDK
                     LELVLKKMAQALAANTNYAAMISGPSYHKTKLKFIQLSRVEANKLLVVVVVEGNIIKN
                     TMIDIGEDLGEQELLNLNILLNSSLNGLTIEEINLGIISKLKEDAGIHSKVIELVLNE
                     VAEAIKAGGEDLQIYTSGATNIFRYPELAEGEKASKLIGTLEQKEVLQEFVSDVNADQ
                     QGDGGIQVYIGDETPIQSMKDCSVVTANYDLGGGLRGTVGIIGPKRMDYEKVLGTLKN
                     LMNQLDAAFKKDER"
     gene            10004..10339
                     /locus_tag="ACZPCL_06330"
                     /pseudo
     CDS             10004..10339
                     /locus_tag="ACZPCL_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587664.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
     gene            complement(10562..12886)
                     /locus_tag="ACZPCL_06335"
     CDS             complement(10562..12886)
                     /locus_tag="ACZPCL_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR1162119.1"
                     /translation="MKMIGKLNEKDLKINKKQMILWMGGILAAVILLMVFIVKVEPPA
                     EGISRAEAAKALALVFEDPQTLEDMASERENSSFTDKEKGNWFVKYMDYLYEKGYLDT
                     EITPATLTSAQGNLTYEDVNTVAEKLSSGLEKQVGMTRGNKKKTFSRNDWWELYKAIV
                     KETDKDGNMKEVSAVLFGTPSNMEQAESWTAYTTEGTFGFQGLALDAYLDCEIRFWAR
                     GQEMAGMTQIVSEEPVYKNIWVSDVKKDQFTVYIGKYVRTFTAEGKLASQAEEKNEEL
                     RSCVADLHMEKGKLKKITVKKERVRGKVLAVTDESIELEGYGCVPIDDNFHVYKTYGD
                     FQVLGKGNILVGYDLQEFVAADGKLCAAVLEQPFDAETIRVLIMDNGFKQIFHDTIEL
                     TANCDGELIYEKENGENQESSFKKGDTFSYEASDKKLENGRMILKPEDSEGITVTSLE
                     RGQGQPTYSGSIEVKAEEGGLVLINELYLEDYLKKVVPSEMPASYEKEALKAQAVCAR
                     TYAYRQIQGNAYGQYGAHVDDSTNFQVYNNISTSERTDQAVNETCGQMLFYNDKPIEA
                     FYYSTSCGHGADGSVWGNEGAALPYLRSMQIKKGARELTMEDNDTFDDYIRSKNITSY
                     DASYPMFRWHVDIKASILSAQIPGVGNVTDVNVVSRGLGGIASEVEVKGDGGSYKIKG
                     QSQVRSMLGNTELVIKKQDGSDMTGTAALPSAFISVEKRTAEDGSIVFRVYGGGFGHG
                     VGMSQNGAHSMAKAGKTYKDILDFFYHGAEIRTE"
     gene            complement(12976..13156)
                     /gene="ssrS"
                     /locus_tag="ACZPCL_06340"
     ncRNA           complement(12976..13156)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACZPCL_06340"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(13311..13808)
                     /gene="cdd"
                     /locus_tag="ACZPCL_06345"
     CDS             complement(13311..13808)
                     /gene="cdd"
                     /locus_tag="ACZPCL_06345"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR1162120.1"
                     /translation="MAYYADNIRKEKRNMEKKQAVVTSDTLTKEQIRELAAKSLAGIK
                     NSYAPYSHFHVSAVLLSENGNIYTGNNIENAAYTPSVCAERCAFFKAVSEEERDFAAI
                     ALCGGLKGVVKDYCAPCGVCRQVMREFCKPDFKIILVKSEEEWKVYTLSQLLPEGFGP
                     ENLRA"
     gene            complement(13910..>14381)
                     /gene="ybaK"
                     /locus_tag="ACZPCL_06350"
     CDS             complement(13910..>14381)
                     /gene="ybaK"
                     /locus_tag="ACZPCL_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009537776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HIR1162121.1"
                     /translation="AEVRTNVMRLLDAAGIHYETGTYEVDENDLSGSHAADLMGIDHD
                     QMYKTLVLKGEKKGYLVCCIPVDEELDLKKVAKAAGEKKVEMIHVKDMLGITGYIRGG
                     CSPIGMKKKFPTYIEETAQLYDTIMVSAGQRGVQVTVSPEDLREYVDGTFVGLV"
BASE COUNT         3466 a         3640 c         2756 g         4519 t
ORIGIN      
        1 tttaccttac gttcaaaatc ctcgtaatct acatgccagc ttccgttttc ttctaccaga
       61 ggattttcta ctactacacg gccaattgcc tctggaatat cataaaattc ggaatataca
      121 ggagtctgga tcaggacctt atctcctggc atggtgtaga tcttcatcag agctgccatg
      181 gtctgaacaa caccaaggca ccagctcatg tgggaagttt caggcttcca gccataatat
      241 ttttcttccc attcctgtac tgcttcaaag tagcttgccg gtctggaagt atatccccag
      301 ataccctctt ccgctttctt tttgcatgca tcaatgatcg gctgggcagt cttaaaatcc
      361 atatcagcta cccataatgg gatcacatct tcggttccga acttcttaag tctttcatca
      421 tatttagaag aacggttgtt gcttctgtcg atcacttcat caaagttgta cttcattgct
      481 gtttcgtatc ctttctttcc cgtttccggt tgatacttat atattaagca agttccatgc
      541 caacttttat tttccccgat tttttattgt tttttgttaa aacaatggtt tttccactta
      601 ttttctggct ttttggaaaa ttttggtaat ttaaggacaa gtcattttca ggtattaaat
      661 ggttaattgg ttatattggt tgatttatta gttattaaat ggttgcctaa ttggttgtaa
      721 cctttttata aatgattcaa aggatcattt ctgtttttcc agttttttca accctttttc
      781 cgtgatccga cttccgcctc tgccccttcc aacctggagc agcccttctt tttccaactc
      841 tttcaaaaga tctcttgcct gtttctgggt cataggaatg tgatcttttt tcgctgcctc
      901 taatattttc tcccttccca ctgtttctcc agctttccag gaaagtgcca gctgttttaa
      961 tacaaactgc tttttagcct ctgtatccat atgaccggaa taggttgaac cttctttcag
     1021 ctgtgtggtt cctgttttca cgttttcatt ttccccactg ctttcacctg gtcctactac
     1081 atccagcccc actcctacta ctctggtcat ggtaggcggc aggtcggaaa gctcgatcac
     1141 cggttttgcc agataattaa aatattccac tgcattctgc agttctctga tgtttcccgg
     1201 ccatggatgc ctgcgcagaa aagccttcac ttcttctgat aaagtaaagt ttccacccat
     1261 tttccgcata aagaaatcaa acagcataaa aatatcatct ccccgctttc tgagaggcgg
     1321 tatctctact gtcagtgcat ttaaacggta atacaggtct tttctgaaac tgccttcctg
     1381 tatcttcttc tctaaggctt cattggtagc tgccacaata cgcacatcca cacggataat
     1441 gctgccgccg cccacgcgca tgatctcccc ttcctggaga acacgcagaa gttttacctg
     1501 catggaaagg ctcattcctt ccacttcatc taaaaataaa gttccgtggt gggcaaattc
     1561 aaaaagaccg ggacggccgc cttttttggc tcctgtaaag gcgccttctt catagccgaa
     1621 caactcgctt tccagaagat tttccggcat ggcagccaca ttaatggcaa taaaaggacc
     1681 ggctgcccgc ccggaagcct gatggactgc atgtgccatc agctcttttc cagtacctgt
     1741 ctctccaatg agaagcacag ggctttcagt tgctgccatt ttccttaaaa tattaatggt
     1801 ttctgtaata gtatctgaat ttcctattac gtcatcgaag gtataacggg cataatggcc
     1861 tttctgcatc agctgtcttc gcaaggcatt ctggcgggac tcctgctcat taaacttctg
     1921 tagcatggca aaggcgccga tacagtcctc ttttcgcacc accggcacca ctgccaggga
     1981 aacattggtt ccaaagaggc ggatcacctt ctcaggaact gcctttttct cccggagcac
     2041 ctgataaaaa ggaatatagg gaaatacctc ttcccccttc tttcccatag ccagttcttc
     2101 agaaatgcgg gcgatctgac aggccttttt gttacagaca aaaacctctc ctgtttcatt
     2161 tacaccgatc agaccttcat ccaggctttc cgccagaata tgcatctggc tttcctgcct
     2221 gcggctttta gcatacatca ggtcaaagct gtagtgattg gaggccacta ccttagcata
     2281 attcataaag ctttctgttt ccggcagata ttccagtccc agacgcaaag ctatttctac
     2341 catcattcca taggaacagg gacggtgatc acagtcgatc actgttttta cagaaggcgg
     2401 cacaaaacgg gattcaccgg gagttaccgc aatatccacc ggttcttcta aaacagcgcc
     2461 gggataataa ggaatgaact gcagatggtt tactccaaga gagcttaact gggtaatagc
     2521 ctctcttgcc atattactgg taacattcac aaacaacact ttcgttcctt gtggtatctc
     2581 aaacagtttt tttagggttt cccagtaaaa agtaacctca atggacatga tctgactatc
     2641 aataggcaca tggcgggcct cttcttctgc agaaccatag gcatctgtag atatagcaaa
     2701 cagatccgct cttggaagca ctccggcagc tgatccatct cttacgctat accccactac
     2761 ttccgcatat tcatcaaaaa gatctcttac ttcttttgta taggaagcac cggccaacgg
     2821 atccagcgcc acaactgcaa tttttttcat cgtattttac accaaccctt ttatatactc
     2881 ttaaaatcct gttacggttc atgcggttca tgcgctgcat gaccagcaac ggatctgcca
     2941 aaaaacgtat ctgttttttc ttccgtattt atgataccat tgatgatatt cctaaaacaa
     3001 ctgttttata aacacaatac tgtttggagg cattttatga catttacata taagatcccc
     3061 tgttttgatc tggagcagac tgccgggtca ggacaggtct ttcgaatgat accggcgcct
     3121 gggcgtacaa atacctggca ggttataagc cagggccatg tttgctttat tcgacaggaa
     3181 aatgacatct tttttctgga atgtggaccg gatttagaaa atttctggaa gaactatttt
     3241 gacttaaata cagattatga gaaaatgatc gcatccatca caccggacga tcactatctg
     3301 aaagcagcct ctaaagccgg acagggcatc cgcatccttc gtcaggatac atgggaaatg
     3361 atcattactt ttgtgatctc acagcaaaag acgatccctg cgatccgggc gttggtggaa
     3421 gccctctgca gccggtatgg gacaaagatt gaccagtttt atgcatttcc cacacctgaa
     3481 cagctgtgtc aggcttcttt agaagatctc cttgccctga aacttggcta ccgggccaaa
     3541 tatatctacc gcctctgccg ggacgctgta gccggacgtc tggacctggc gcatttaagc
     3601 aagttggatt ataccgctgc catggagtat cttaccggct tttacggcat tggcaagaaa
     3661 gtagccaact gtgtctgcct tttcggtttg catcacattg acgcctttcc tgtggatacc
     3721 tggattgaga agatcctaaa cgaacattat tatgatgctg aaaaatacaa agatctgcct
     3781 aaagccagac tttatgacca gatgatcacc gactcttttg gatgttataa gggctatgcg
     3841 ggagtcatgc agcagtatat ctttaattat gaacgcaatg taatacacgg gaaataattt
     3901 gcagatccgt gagcgcctca ctgcgcactt tcaatatgta tgacaacaaa aaacgagaag
     3961 aggcatatct catcctcttc tcgttttttt gatctatctt agattcattt atttctctgt
     4021 gatctcaaat atcttccagc ttttccggct tcgtcccact gcatactgcc ttgtgatatt
     4081 ttcctttgtt tcatccggcc actgttcatc taaggaaacc tggactactt tataggaagt
     4141 atagccaaga agtgtggcat ggttgtcata ctgggatttt tcatccccgt attcctcttc
     4201 ggataaggtc ttaaaaccat gcaccagaac gtcctgtgga taaataccat tttcaaaatc
     4261 agcctgcata ctatcaaaag gaacattttt tgccagatca tcagaaagga ttttcccgct
     4321 ttcctcaaac tggaccattg cattataccg cagatacatt cccactgcct gttctgcttc
     4381 acctaccggc atggactcaa atttgatatg ccttggcttt gcgtcatctg taatagtata
     4441 ttcctggctt aaccggttta atgcctcatt ccggctgtgt actgcgattg caagcatcag
     4501 tccgaaacct accactgtca gaataaagat cttccagggc ggcactgtat tttttctctt
     4561 ttttatcagc atattgcaga ggttctccta tttaaaaagc cctttcttct ttggcttttc
     4621 tccttctggt tcaattccgt tcatcaggtc atcatagcgc tttaatgcat ccttctgagc
     4681 ctgattcata cgctccggca ccttgactac cagtgttaca taatgatcgc cgcgcacatt
     4741 gcggttacgc agggatggaa cgccttttcc ctttaaacgt accttggtat cggtctgggt
     4801 acctggtttt acctcatact ctacttcacc gtctacggtc ttgatgcgga tcggaccgcc
     4861 taatgctgcc tttgcaaagg agatcggtac agtagaataa atgctggtat cctggcgctt
     4921 gaagattgga tgctgggata ctactgcctc tactaacagg tcgcctctct cgccgccgtt
     4981 tgttcccggc tcaccgccgc cagccatacg gatacactgg ccattgtcaa tacctgccgg
     5041 gatagaaact ttaaatttct tgcgaacagt cttgtatccg gtaccgtagc agttcgggca
     5101 tttttccttg ataaccttac cagtaccatt acactctggg caggtctgaa cattctgtac
     5161 ctggccaaag aaagactgct gtgtatacat aatcttacct ttaccattac acttggtaca
     5221 ggtctgtggg gaagtacctg gctttgcgcc ggttccgtta cagttggaac atacttcttt
     5281 atagttgatc tcgatctctt tttcgcagcc aaatacagcc tcttcaaagg taatgcggac
     5341 tgctgtcctt acatttgcgc ctttcatagg gccgcttctg gatctggaac ggccgccgcc
     5401 gaaaatatca ccgaaaatat cgccaaagat atctcccata tctccgccgt taaaatcaaa
     5461 gccgccgaaa ccgccgccat aaccagcgcc gccgccctgc tcaaatgcag catggccgaa
     5521 ctggtcgtac tgctgacgtt tctgaggatc gctcagtacg ctgtaagcct ctgaagcttc
     5581 cttaaacttt gcctctgcct ccttgtcacc aggatttgcg tcaggatggt atttcttggc
     5641 aagttttctg tatgcttttt tcagggtatc ttcatcagcg tcttttgaaa cacccagaac
     5701 ttcatagtaa tctctcttat tctctgccat gttgtttacc tcttatcact gctatttctt
     5761 tctctgccgg gtccaaaatg ccctttgttt aaacagacgg taaggcccgg aaggggatat
     5821 ctcccctctc ggacccgtcc gttagttcag acatgccagc ttgcaacaca ctttcccgtc
     5881 caaaatggcg catgtctgaa ctgttatata atcttacact tctttgaagt cagcgtcaac
     5941 tacatcatca tctggtttgg agcttcctgc attgcctgca tttgcaccac ccatgtttgg
     6001 acctgcacct gcaccagcct gctgtgcctg ctcataaacc tttgcaaaca gcttctgggc
     6061 actgttcatc agtttttctt tacctgactt gatatcttca acctgggcat cagtcatctg
     6121 atcaaccggt gctctgttga tagcttcttt caatgcattt aaatcagcct caacctcagc
     6181 cttgtcgtta gcatcgatct tgtctccaac ttcttccaga gccttctcag tctggaatac
     6241 catagaatca gcatcatttc ttgcatcgat cgcttcctta cgcttcttat cctgagcctc
     6301 gtactcagca gcctctttaa cagccttatc aatatctgaa tcagacatgt tagatccaga
     6361 agtaatggta atatgctgct ctttgcctgt tccaagatcc ttagcagata catttacaat
     6421 accgtttgca tcgatatcga aagtaacttc gatctgagga acacctcttc ttgctggtgg
     6481 gataccatcc agacggaact ggcctaaggt cttgttatct cttgcaaact gacgctcgcc
     6541 ctgtacaaca tggatatcaa cagcggtctg attatcagct gcagtagaga agatctggct
     6601 cttctttgta ggaatggttg tatttctctc gatcagtctg gttgctacac cacccatggt
     6661 ctcaatagac agagacagtg gagttacatc cagaagaagg atatctccgg cacctgcatc
     6721 gcctgctaac ttaccaccct ggatagctgc accgattgca acacactcat ctgggttcag
     6781 ggacttgcta ggctctttgc cggttaactg ttttactttc tcctgtgcgg agatcatacg
     6841 tgtagaacca ccaactaaca gtaccttgcc aagctcagct gcggtaatac cagcatcctt
     6901 caatgcgttc tgtaccggga tagctgtacg ctcgatcagg tcatgtgtca gttcatcaaa
     6961 tttagctctt gtcaggttca tatccagatg ctttggacct tctgcagtag ctgtgatgaa
     7021 tggcaggttg atattggttg tagtcgcagt agacagttcc ttcttagctt tctcagctgc
     7081 ttcttttaat ctctgaagag ccatcttatc gccggaaagg tctacgcctt ctgccttctt
     7141 gaattcagag atcatccagt tggtaattgc attatcgaag tcatcaccgc caaggtgtgt
     7201 atcaccgttg gtagaaagaa cttcaataac accatcaccg atctcaatga tagatacatc
     7261 gaatgtacca ccaccaaggt catataccat gatcttctgc tctttttcat tgtcaaggcc
     7321 gtaagctaat gctgctgctg taggctcgtt gatgatacgc tttacatcca gaccagcgat
     7381 cttaccggca tccttggttg cctgacgctg tgcatcgttg aaatatgcag gaacagtgat
     7441 aactgcttcg cttacagtct cgcccagata gctttcagca tctgctttca gcttctgaag
     7501 gatcattgcg gagatctcct gtggagtgta tttctttcca tcaatgtcta ccttgtagtc
     7561 agtacccata tgacgcttga tagaagcaat ggttctgtct gcattggtaa ctgcctgacg
     7621 cttagcaggc tcacctacca gtctttctcc tgtttttgta aatgccacaa cagatggtgt
     7681 ggttcttacg ccctctgcgt tagcgataac ggttggcttt ccaccttcca taacggctac
     7741 gcagctatta gttgtaccta agtcgatacc aataatctta ctcatagttt tttcctcctg
     7801 ttatatatgt ttgatgaaaa aatcataata aatgttccac tcaactaagg ctcgaaaaga
     7861 cctcgccaag tttcgggaac gactttcgca ctaaactcgt gaaagacctc gttaagtact
     7921 caatgtcagt tcgctacttt aaccatgctg tgtcttacaa cactgtcacg gtacatatag
     7981 cctttctgaa gttcctcagc tacgatattc tctcctaagc tttcatcttc tacatgcatc
     8041 actgcattgt ggaagttagg atcgaatggc tgtccggctg cctcaatggc ttttacaccg
     8101 gcgtcttcta agactttctg gaactgctta tatatttttt ccattccctc agcaaaagct
     8161 gtattttttt cgctctccgg aatggaagct aagcctcttt caaaattatc aactaccgga
     8221 agcatacgct cgatgatgtc ttttgcaccc atctcgtaca tggtagattt ttctttttcc
     8281 gttctcttgc ggaaattatc aaattctgcc atctggcgtt ttacccggtc ggtaagttct
     8341 tctatttttt catccttagg atcttttttc tttttaaaga agccctcttt ctcaggtgct
     8401 ttttctgtgg tcttctcttc tgcacttgtt tcagtgttct cagaagcagt ctcctcagaa
     8461 gcttcctgtt ccatattgga cggatccttc agatcttcat ttttcatttc ttcgctcact
     8521 gctatcacct ttcatccttt ttaaatgctg catccagctg gttcatgaga tttttcaaag
     8581 ttccaagaac tttttcataa tccattcgct taggaccgat aattcctacg gttccccgaa
     8641 ggcccccgcc cagatcataa ttggctgtga ccacgctgca gtccttcatg gactggatcg
     8701 gtgtttcatc accaatgtat acctgaatgc ctccatcgcc ttgctggtct gcgtttacat
     8761 cggaaacaaa ttcctgcagc acttcttttt gttctaatgt tccgatcagc ttgctggcct
     8821 tttctccttc agccagttcc ggataccgga aaatgttggt cgctccgctg gtataaatct
     8881 gcaggtcctc gccgcccgct ttaatagctt ctgcgacttc attcagtacc agttcgatca
     8941 ccttactgtg aatgcctgca tcttctttca gcttgctgat aatcccaaga ttgatctctt
     9001 caatggtcag tccgtttaag ctgctgttta agaggatgtt cagattgaga agttcctgtt
     9061 ctcccagatc ttcgccaata tcgatcatgg tgtttttgat gatgttgcct tccacaacca
     9121 ctaccaccag aagcttattt gcttctactc tggaaagctg tatgaatttc agctttgtct
     9181 tgtggtagct tggtccgctg atcatcgctg cataattggt atttgccgca agagcctgag
     9241 ccattttctt aaggaccagt tccagtttat ctactttctg gaccatcaca tccttaaatt
     9301 ctgtgacttc gttttctttc tcctgcatga tctggtcaac gtagaacctg tatcccttat
     9361 cagaaggaat acgtcctgct gatgtatgtg gctgtataat gtagcccatc tcttccaggt
     9421 ctgacatctc attgcggatg gtggcagaac tgagtttcag ttccgaatac ttggaaatcg
     9481 tccgggaacc caccggctcg cctgtttcca gataagtctt tatgatcgct tttaatatag
     9541 tgaccttacg ctcatccaac tgcgcatcca tgtgttcctg cctcctgttc gctgtttctt
     9601 ttgttattag cactcaactt attggagtgc taacatctac attgcataat atattccttt
     9661 ataaagaatt tgtcaagggc tttttgcgca ttttgtgttt ttcttgacag atttaaaaaa
     9721 atgtggtaaa tattacttta tcgttcatat attacaaaag tgttacatat atttacaatg
     9781 tattaaaaat acattgcaaa tattatgagg cattgtgaac gaccgcagtc cttgtccttt
     9841 ccgactgcaa aaggaggtag aaatgaacat gaagaaacta ttaaccacga cttttatcac
     9901 tgtatttttt ataattggct ctgccttttg tgcattcgct tctgaaaatg acagttccga
     9961 acaggcacag caaacagaag aagctgcaaa cgatcaggat cagcaggaaa cagaagctgc
    10021 cgaaaacgtt gaagaaccgg cttcccagat ggtttccctg ggtgttttta aaactaccgg
    10081 ttactgccct tgcagaagct gctccgcagg ctggggaaga aacacctgta ccggtgccgt
    10141 tgccagctcc cagcacacca ttgctgttga cccaagagtg atcccatatg gaaccagggt
    10201 tatgatcaac ggggtggttt acattgccga ggacaaaggc ggcggtgtaa aaggccatca
    10261 cattgatatt ttttatgata cacacgcaga atcaaaaaat cacggaatac agaatgtaga
    10321 ggttttctta ttatcctaac catatgatta aataatatat aaagccagtc tgccagctgc
    10381 ctttggcagt ggctaacaag atattggcaa aagctgtcac cggcagctgc ttccagatac
    10441 ataacaacta aaaaacacgg tcaggaaata tttccgtttc ctaaaccgtg tttttttatg
    10501 cccgtattct gttttcccag accaggtctc tgcctccggc tgccagccgg acacatcggt
    10561 cttattctgt cctgatctcc gctccatgat agaagaaatc aaggatgtct ttatacgtct
    10621 ttcctgcctt tgccatgctg tgggcgccgt tctggctcat tccaactcca tgtccgaaac
    10681 cgccgccgta tacccggaat acaatgcttc cgtcctctgc cgtgcgcttc tcaacggaaa
    10741 taaacgcact tggaagggct gccgttcctg tcatatcgct tccgtcctgt ttctttatta
    10801 caagttccgt atttccaagc atacttctta cctggctctg gcctttgatc ttgtagcttc
    10861 ctccatcgcc ttttacttct acttcagaag caatgccacc taaacctctg gatactacat
    10921 ttacatcggt tacatttccc actcccggga tctgtgcgga aaggatactg gctttaatat
    10981 ccacatgcca gcggaacata ggataagaag catcataaga agtaatgttt tttgaccgta
    11041 tgtaatcatc aaaagtatca ttatcttcca tagtcagctc tcttgcgcct tttttgatct
    11101 gcatggaacg caggtagggc agagccgccc cttcattgcc ccatacactt ccatctgccc
    11161 catggccgca ggaagtggaa taataaaagg cttctatggg cttatcatta tagaaaagca
    11221 tctgtccaca ggtctcattt actgcctggt ctgtacgctc gctggtactt atattgttgt
    11281 acacctggaa attagtgctg tcatccacat gggcaccata ctgcccgtag gcatttccct
    11341 ggatctggcg gtaggcataa gtccgggcac acactgcctg ggcttttaat gcctcttttt
    11401 cataggacgc aggcatttca cttggaacta ccttctttaa ataatcttcc aggtacaact
    11461 cgttaataag gactaagccg ccctcttctg ctttcacttc tatagaacca ctgtaagtag
    11521 gctgtccctg gccacgttct aaggaagtga cggtaattcc ttcactgtct tccggtttta
    11581 agatcatacg tccgttttcc agctttttat cagatgcttc gtaggagaat gtatcccctt
    11641 tcttaaagga gctttcctga ttttctccgt tttccttttc gtagatcagt tccccatcgc
    11701 aatttgcagt aagttctatg gtatcatgaa agatctgctt aaaaccatta tccatgatca
    11761 ggacgcggat ggtttccgca tcaaatggct gttccagaac agcggcacaa agctttccgt
    11821 cagctgccac aaattcctgc agatcataac ctaccaggat gtttcctttt ccaaggacct
    11881 ggaaatcccc gtaggtcttg tatacatgaa aattgtcatc gatgggtacg cagccatagc
    11941 cttccagttc aatagactca tccgtcactg caaggacctt tccacggacc cgttcttttt
    12001 tcacagtgat ctttttcagc tttccctttt ccatatgcag gtcagccaca caggatctca
    12061 gttcttcatt cttctcttct gcctgggatg ccagtttacc ttctgctgta aaagttctta
    12121 catatttgcc gatgtaaacc gtaaactgat ctttttttac atcagagacc catatattct
    12181 tataaacagg ctcctcgctt acgatctggg tcatacctgc catttcctgg cctctggccc
    12241 agaaacggat ctcgcagtca agatatgcgt ccagagccag tccctgaaag ccaaaggtac
    12301 cttctgttgt ataagcagtc cagctttcgg cctgttccat attagaaggt gtgccaaaca
    12361 aaacagcgga aacttctttc atatttccat ctttgtccgt ctcttttaca atggccttat
    12421 aaagctccca ccagtcattt cttgaaaatg tctttttctt attccctctg gtcattccca
    12481 cctgtttttc aaggccggat gacagctttt ctgctaccgt atttacatcc tcatatgtaa
    12541 gatttccctg ggcagaagta agagtcgccg gtgtgatctc tgtatccaga taaccttttt
    12601 cataaagata atccatatat tttacaaacc agtttccctt ttccttgtct gtaaaagagg
    12661 aattttcccg ttcagaagcc atatcttcca aggtctgggg atcttcaaaa accagagcca
    12721 gggctttggc tgcttctgcc ctggatattc cttctgcagg aggttccact tttacaatga
    12781 acaccataag caggatcact gccgccagta ttcctcccat ccacaaaatc atctgttttt
    12841 tgttgatctt tagatccttt tcatttaact tcccgatcat tttcataata tctgtcacct
    12901 tattcgcaca agtatattca gcaagcccat gtactacaaa aagaaagcag cgagaacctc
    12961 gctgctttta tcttctgtga ccccaagatg ccgtctctta cttattgacg cctagcaatg
    13021 ctaggtgggc aacctcactt aaacttctgc cttccacgct taaaaaggag gcatgacatc
    13081 cgcttaaaaa tattggaatc cctttagtct aatgtaggtt caaaattggt aagaacttcg
    13141 cacatcccag gtatcacata tgcgcttcat ttattatggt gctattataa cccaacattt
    13201 tatgtttttc aagcctgttt ttttagttaa tattgttata tattgttagt atttgcgtct
    13261 tgatttcagg tattaagctg aactgtaaca cccgtattgt cctgccggat ctacgcccga
    13321 agattttccg gtccaaaacc ttccggcaac aattgggata aggtatatac cttccactct
    13381 tcttcggact ttaccagaat gatcttaaaa tccggcttac agaattcccg catcacctga
    13441 cggcagaccc cgcagggggc acagtaatcc tttactactc cctttaagcc gccgcaaagg
    13501 gcaatagctg caaagtcacg ttcttcttcg ctgactgctt taaagaaagc gcagcgctcg
    13561 gcgcatacag aaggggtgta ggcggcattt tctatgttat ttcctgtata gatattgccg
    13621 ttttcactaa gaaggacagc ggaaacgtgg aaatgggaat aaggggcata ggagttttta
    13681 atacctgcaa gggattttgc cgccagttcc ctgatctgct cttttgtaag ggtgtctgag
    13741 gtaacaactg cctgtttttt ctccatattg cgtttctcct ttcggatgtt atctgcataa
    13801 tatgccatca aaagcgccgt ccgttttccg tgatacctga tttcatttac aaacctgcat
    13861 taaggtattc tatcttttga gaccgttttg agacgatcgt aaccatcacc tacacaagtc
    13921 caacaaaggt tccgtctaca tactccctca ggtcttccgg acttacagtg acctgtacgc
    13981 ctctctgtcc tgcacttacc ataattgtat catataactg ggcagtttct tcaatatatg
    14041 ttggaaattt tttcttcatt ccgattggag aacagccacc ccggatgtaa ccggtaatgc
    14101 ctaacatatc ttttacgtgg atcatctcca cttttttctc tcccgctgct tttgccactt
    14161 tctttaagtc cagttcttca tctaccggga tacaacatac aagatatccc tttttctccc
    14221 cttttaatac aagggtctta tacatctggt catgatcgat ccccatcagg tcagcagcat
    14281 ggctgccgga aaggtcattt tcatctactt catatgttcc tgtttcataa tggatccctg
    14341 ctgcgtctaa cagcctcatt acgttggtcc ttacttctgc t
//
LOCUS       DBMDUA010000060        14226 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2320_length_14226_cov_2.914973, whole genome shotgun sequence.
ACCESSION   DBMDUA010000060 DBMDUA010000000
VERSION     DBMDUA010000060.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 14226)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14226)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14226
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2320_length_14226_cov_2.914973"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="ACZPCL_06355"
     CDS             <1..289
                     /locus_tag="ACZPCL_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HIR1162122.1"
                     /translation="DMQTIMAGLACGEPNTISWDILKNHVKVFIAAPVKGDAPITSGE
                     SGAAPFGALAAMMTMDEYKDLRNDIGLDENSKVLLFSTEGDTDPDRYKNIV"
     gene            574..1929
                     /locus_tag="ACZPCL_06360"
     CDS             574..1929
                     /locus_tag="ACZPCL_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1162123.1"
                     /translation="MKQPSKDLTQGSLWKNIFVFSIPLMLSNVLQVLFNMSDIAVVGR
                     FAGPLALGSVGSTAILVTMFTGFLIGLAGGINVLTALYLGAGKKQALSHTVHSALIVS
                     ICSGLALLLFGICFARPVLEILHTKEELIDGSVLYLRIYFLGMPALAVYNCGNAIFSA
                     DGDTARPVRYLLLAGVLNVIMNLFFVVICKMDVAGVATASAISQYVSAGLILRDLFCI
                     NTDYRLHLSKLRPDPSKTIDILKIGIPSGLQNAIFALANLFVQVGVNSFSATMVAGNS
                     AAANSDGLIYDVMAAIYTACASFMSQNLGAGKRDRVLKSYLISLLYSFGIGGAMGLLL
                     VIFGKQFLSLFTADPEVITAGLKRLTIMGFSYGVSAFMDCTIAASRALGKSLVPMFIV
                     IMGSCVFRVVWVYTVFAYFKTIPSLYLLYVFSWTITAITEIIYFLHVYRGIFPKANAV
                     A"
     gene            complement(2148..2504)
                     /gene="rplT"
                     /locus_tag="ACZPCL_06365"
     CDS             complement(2148..2504)
                     /gene="rplT"
                     /locus_tag="ACZPCL_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR1162124.1"
                     /translation="MARIKGGLNAKKKHNRILKMAKGYRGARSKQYRVAKQSVMRALT
                     SSYAGRKERKRQFRQLWIARINAAARINGLSYSKFMYGLKLAEVDLNRKVLSDMAIND
                     AEGFAKLAELAKSKLA"
     gene            complement(2534..2731)
                     /gene="rpmI"
                     /locus_tag="ACZPCL_06370"
     CDS             complement(2534..2731)
                     /gene="rpmI"
                     /locus_tag="ACZPCL_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR1162125.1"
                     /translation="MPKLKTSRAAAKRFKKTGTGKLVRNKAYKSHILTKKSTKRKRNL
                     RKDIVTDATNVKAMKKILPYL"
     gene            complement(2761..3255)
                     /gene="infC"
                     /locus_tag="ACZPCL_06375"
     CDS             complement(2761..3255)
                     /gene="infC"
                     /locus_tag="ACZPCL_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR1162126.1"
                     /translation="MINEQIRDREVRLIGENGEQLGIMSARDAFKLAQEAELDLVKIA
                     PTAKPPVCKIIDYGKYRYELARKEKEARKKQKVIDVKEVRLSPNIDTNDLNTKVGAAR
                     KFLEKGDKVKVTLRFRGREMAHMSKSKYILDDFAESLADIAVVDKPSKVEGRSMVMFL
                     TAKR"
     gene            complement(3573..3887)
                     /locus_tag="ACZPCL_06380"
     CDS             complement(3573..3887)
                     /locus_tag="ACZPCL_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HIR1162127.1"
                     /translation="MTKLECSAKNCVHYAEGCCCKSSITVDGAHARCADDTCCASFSE
                     NTGGVFKNLFKTPESKLDVVCDADSCRYNEDHCCCAGKIDIRGEGACDCGETCCGTFE
                     QG"
     gene            complement(3988..5625)
                     /locus_tag="ACZPCL_06385"
     CDS             complement(3988..5625)
                     /locus_tag="ACZPCL_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162128.1"
                     /translation="MKINLRRVTTGAVLIAAASAFLFCTGFTKASDHGNHENGYLKSA
                     TYYSDDWVINFWNSESSHMDEELKQIREDGFNSIILVVPWREFQPSMAPEHYNDYAWE
                     KFDRVMKAAKAQDLKVMLRVGYTWDYYSSENVLARYEKLLYDSETKKAWLHYAKRLYE
                     KASAYDNFSGGFITWEDFWNFAENGSTLYGSGITGRKMAAQCGYTQYVKEHYTLEELG
                     EIYRDTFDSYGEIYLPAKESYARKLFFQFYDHFLNDILAETQTVFPDLSMEVRLDVDP
                     VRRLDGTLEGAPHDATFSCGSSGFASAMFSVPMGFENNGEKVTAAQALSNAASYFDRL
                     FQSSQQKKIYIDQFLFTDNTPGFEHNAQLLETEKAAYLDGMAELFKAKTLGYGIWTYR
                     DYGDNKVYNSQFALAQQGWKFSGGSKVEEHNGSRMAVLPVSGWISQDIHNRSAGMEEN
                     NLYVSFLLEGEGNCRVMVQAGGQKQTVSAGSPQTVTLNFGTASAGELVISAQGTGTVY
                     IDDVKVYTGITQGDMYHMDGTESSCIQAVRAMNAKVR"
     gene            complement(5622..7043)
                     /locus_tag="ACZPCL_06390"
     CDS             complement(5622..7043)
                     /locus_tag="ACZPCL_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1162129.1"
                     /translation="MKQKENVKEEVKEEAGEKQQKNNFAQGSIVVNILKLAVPMTLAQ
                     LINVLYNIVDRLYIGQLKDEATISLTGLGLCLPIISIVIAFANLFGMGGAPLCSIERG
                     RGNNEEAEAIMGNSFAMMVVVGVILTVVGLVFKRPMLYLFGASDATIGYADSYITIYL
                     MGNIFVMTGLGMNSFINSQGFGTIGMMTVLLGAVANIILDPVFIFFFHMGVRGAALAT
                     ILSQLISAIWILKFLTGPKAILKLKKNCMKLKRSRVLRIIGLGMSGFTMSITNSSVQV
                     MYNAMLQKTGGDLYVGIMTVINTVREVISMPVQGITHSSQPVMGYNYGAKEYGRVKKA
                     IVFVSAVSVLYTLGVWSLVHAFPEFFIRIFNRTGDLVEAGIPAMRIYFFGFFMMAFQF
                     AGQAIFVGLGKAKNAVFFSIFRKVIIVIPLIIILPSMFHMGTDGILMAEPVSNFIGGT
                     ACFVTMLCTVWPELTKGERDKNK"
     gene            complement(7097..7717)
                     /locus_tag="ACZPCL_06395"
     CDS             complement(7097..7717)
                     /locus_tag="ACZPCL_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1162130.1"
                     /translation="MKVSMIYMASGFGKRYGTNKLITEFRGEPLYLHGLLSLQKAAKL
                     LEKTEKINCQLILISQYERVLSGSVEQVPEAERFFNEDSDQGITASIKLGTEVAWDNA
                     DAYLYFVADQPCMAGETIARFVSEFMKSKKGIGCVCAKGHRGSPNIFRRRYKKELLAL
                     EGDQGGRQIMQKYPQEIWMMEVDERELKDIDQPADLTALRLELENT"
     gene            complement(7757..8560)
                     /gene="yqeC"
                     /locus_tag="ACZPCL_06400"
     CDS             complement(7757..8560)
                     /gene="yqeC"
                     /locus_tag="ACZPCL_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HIR1162131.1"
                     /translation="MKISVKTQGKWTQTDTLWSALFIDDRLKKDPGQADHSPVLSFVG
                     GGGKTSYIRRLAWEGREKGCRVLVMTTTHMAVPEYFGVLEPDLGQVEKMLEKEGIAVA
                     GCPAKEGKIAFRDWEFYEKAGKLADVILVEADGSKRLPVKVPGPKEPVTPENSDQILC
                     IYGLGALGKQAADCCFRMQPSEQLLKIPEDQKDGKWIMTEEKMSLLMKKGYLEPLRQQ
                     FPYSQVIPVFNQADTMKLEENGKNILERMEEERGILTGELWKETSFNLF"
     gene            9235..9519
                     /locus_tag="ACZPCL_06405"
     CDS             9235..9519
                     /locus_tag="ACZPCL_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HIR1162132.1"
                     /translation="MSVVIIGGHDRMVSQYKKICKNYNCKAKVFTQMSASLDKQIGRP
                     DLLVLFTNTVSHKMIRCALDEVNEQETEIVRCHTSSGTALTEILMQHVPA"
     gene            complement(9629..10801)
                     /locus_tag="ACZPCL_06410"
     CDS             complement(9629..10801)
                     /locus_tag="ACZPCL_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1162133.1"
                     /translation="MIYLDSAATSFYRPPCVAEAVAEAISSMGNCSRGAYKEALTGAR
                     VIFETRVLLSEMFHGDGPEQVAFTANSTESLNIAIKGLIRPGDRVVTTVLEHNSVLRP
                     LYEMEKQGAELVIVGCEQAPESAGEEEKERVRRLGKLDYKALKAAITPGTRAVVMTHA
                     SNLTGNRTDLKKVGEWCRQAGALFIVDASQTAGHFPINIEENHIDVLCFTGHKGLMGP
                     QGTGGLCVRKGLAVKPLLSGGSGILTFEKQHPSVMPTALEAGTLNGHGIAGLRAALLY
                     IKEEGIEAIGAREQKLMQLFYEQVKEIPDIRIYGDFSQKDRAAIVSLNLGEEESGEVS
                     DYLSTEYGIDTRSGGHCAPLMHGALGTVDQGAVRFSFSHFNTEEQVECAVRALKEY"
     gene            complement(10814..11647)
                     /gene="yqeB"
                     /locus_tag="ACZPCL_06415"
     CDS             complement(10814..11647)
                     /gene="yqeB"
                     /locus_tag="ACZPCL_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007037836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HIR1162134.1"
                     /translation="MIQRNEQLVIVRGGGDIATGTISRLFRSGYPVVILEISTPSAIR
                     RQVALSEAVYDGESKVEDVTCIKVESWEEAKEVLKEKKKVPLLVDPECNILKQVRPWA
                     LVDAILAKKNLGTNRQMADKTIALGPGFSAGVDVDLVVETQRGHNLGRIIEKGPATPN
                     TGTPGIIGGYGKERVIHSPAEGKLYGRVKIGDKVEKGQTIAVICTENGEEVKVEATLT
                     GLVRGLIRDAYPVTVGFKIADIDPRESEYKNCFTISDKARNIGGSVLEGLMYLEEKQG
                     Y"
     gene            complement(11715..12335)
                     /gene="yedF"
                     /locus_tag="ACZPCL_06420"
     CDS             complement(11715..12335)
                     /gene="yedF"
                     /locus_tag="ACZPCL_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HIR1162135.1"
                     /translation="MRLDERGKQCPLPVIEAKKALEASAPGEKVEVVVDNEIAVQNLS
                     KMADHKGLAVCSEKTGEREFLVTITAGEGNGEAAEAPVAAEEVTCTPDGRRKGMVVVL
                     SSNQMGQGDEALGKLLMKGFVFALTQQDMLPETILLYNSGAFLSCEGSDNLEDLKTLE
                     AQGVEILTCGTCLNHYGLAEKRQVGGVTNMYEIVEKMTKASVVVRP"
     gene            complement(12436..13461)
                     /gene="selD"
                     /locus_tag="ACZPCL_06425"
     CDS             complement(12436..13461)
                     /gene="selD"
                     /locus_tag="ACZPCL_06425"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR1162136.1"
                     /translation="MEPQEIVFCKGGGCTAKLGPGILARVLNNLPKTYDPNLLIGFDG
                     SDDAAVYKLTDDLAIVQTLDFFPPMVEDPYIFGQIAAANALSDIYAMGGDVKTALNIV
                     CFPEAMDLNILGKIMLGGSEKVREAGGVLAGGHSIADSDVKYGLSVTGVIHPDKVFAN
                     SGCKVGDALILTKPLGVGIVCTASRMKAASKEAYDLAVKSMTTLNKYASEILRKYRLH
                     GCTDVTGFGFLGHLCEMVTDQATARIYKEKVPYIPECEDYVEEFYLTAAAQRNRNHVQ
                     DKVEFRNCSFATEEILYDPQTSGGLLASVDKEDVPAIMEELEKLGLPCGVVGEILPKQ
                     EKAVIIQ"
     gene            complement(13466..13840)
                     /locus_tag="ACZPCL_06430"
     CDS             complement(13466..13840)
                     /locus_tag="ACZPCL_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1162137.1"
                     /translation="MGESRLHYGMSLRLYFEEKAFGPGMVMLLREVERTGSLQKAAKA
                     MNMAYSKAWKMLKGAEKEWGFALTDRETGGKDGGGSTLTPQAERLIEAYEGFKKDAVD
                     HLDQLFAQYFSEEKVEKIKGME"
BASE COUNT         3565 a         3606 c         2669 g         4386 t
ORIGIN      
        1 cgatatgcag accatcatgg caggtcttgc atgtggtgaa ccgaacacca tttcctggga
       61 catcttaaag aaccatgtaa aagtattcat tgctgctcct gttaagggcg atgctccgat
      121 cacctccggt gagtctggtg ctgctccatt cggtgcatta gcagctatga tgaccatgga
      181 cgaatacaaa gatttaagaa atgacattgg cctggatgaa aattccaaag tactgctctt
      241 ctctacagag ggcgataccg atcctgatcg ttacaagaac attgtatgaa aaggccttga
      301 taaataatta caaaacagga aatacatgtt taaaacataa ccactaaaag agatccttct
      361 atgatatgtt cgatatctat agaaaggatc tctttaatat cataaatact cataaacaaa
      421 agaaactttt atttttccat ctcttgtaca caatatcccc ttatgctata ctgaagctag
      481 tgctgataac acagaaaact ctgtgttatg ggtctttttt tatctttata aaatttcata
      541 tataaaatta tttttacgaa tggagaagtt tttatgaaac agccatctaa agatctgact
      601 cagggatccc tttggaagaa catttttgtt ttcagcatac cgctgatgct ttccaatgtc
      661 ctccaggttc tttttaatat gtcggatatc gccgttgtag gacgttttgc ggggccgtta
      721 gctttggggt ccgttggttc cactgcgatc ctggtaacga tgtttacagg ctttctcatt
      781 ggtctggcgg gtggtatcaa tgtacttacc gccctctatc ttggcgccgg gaaaaagcag
      841 gctctttccc atacggtcca ctctgctctt atcgtcagta tctgcagcgg tctggccctg
      901 ttattattcg gtatctgctt tgcaagacct gtccttgaga tcttacatac aaaggaagaa
      961 ctgatcgacg gctctgtgct gtatcttcgc atttacttcc ttggaatgcc tgccctggct
     1021 gtttataact gcggtaatgc tattttcagc gcagacggtg atacagcaag gccggtacgc
     1081 tatctgctcc ttgcaggcgt tttaaacgtg atcatgaacc tgttcttcgt cgtcatctgc
     1141 aagatggatg tagccggtgt tgcaactgcc agtgccatat cccagtatgt atctgccgga
     1201 ctgatcttac gggatctgtt ctgtatcaat acagattaca gactgcattt atcaaagctc
     1261 cgtccggacc cgtcaaagac catcgacatc ttaaagatcg gtattccttc cggtctgcag
     1321 aatgctattt tcgcccttgc aaacctgttt gtacaggtag gtgtcaattc cttcagcgcc
     1381 actatggttg caggaaattc tgcggctgca aactccgacg gcctgatcta tgacgtaatg
     1441 gcagctatct ataccgcatg cgccagcttt atgagccaga acctgggtgc cggaaaacgt
     1501 gaccgggtat taaagagcta tctgatcagc ctgctgtatt ctttcggcat cggaggtgct
     1561 atgggactgc tcttagttat cttcggaaaa cagttcttat ctctgtttac agctgatcct
     1621 gaagtcatta ctgccggtct taagcgcctt accatcatgg gcttttccta tggtgtttct
     1681 gcttttatgg actgcaccat tgccgcttcc agagcccttg gaaagagtct ggttcccatg
     1741 ttcatcgtga tcatgggttc ctgtgtattc cgtgttgtat gggtctacac tgtatttgcc
     1801 tactttaaga cgatcccgtc tttgtatctg ctttatgttt tctcctggac cattacagcc
     1861 attacagaga tcatttactt cctgcatgta tacagaggga tattccccaa ggcgaacgct
     1921 gtggcctgat ctgatcacat cagattcgct tcaaaacatt gtatttatcc caaaaatctt
     1981 caaataccgt ctgctgccag ttctgttgga tgcaggcggt atttctttac tttgaacctg
     2041 ctaccggcag cttccttcag atgcataaaa tgcatggcaa caaaaaaaca ccaggaacaa
     2101 cttttcagtc attcccggtg cttactattt ataataattc tgacagatta agcaagtttg
     2161 ctctttgcca gttctgctaa cttagcaaaa ccttctgcat cgttgatagc catatcggac
     2221 agaaccttac ggtttaaatc aacctcagcc agctttaagc cgtacatgaa cttgctgtag
     2281 gacagaccat tgatacgagc tgctgcgttg atacgagcga tccacagctg tctgaactgt
     2341 ctctttctct cttttctgcc tgcgtaggag ctggtcagtg cgcgcataac ggactgctta
     2401 gcaactctgt actgcttaga acgagcgcct ctgtagccct tagccatctt caggattcta
     2461 ttgtgcttct tctttgcgtt taaaccgcct ttaattcttg ccatcggtaa ttcctccttc
     2521 tacaatctaa aacttacaga tatggtaaaa tcttcttcat tgcctttacg ttagtagcat
     2581 cggtaacgat atcttttcta agatttctct ttctcttagt agacttctta gttaagatgt
     2641 gagacttgta agctttattg cggactaact taccggttcc tgtcttttta aagcgctttg
     2701 ctgcagctct gctggttttt aactttggca ttgtaaattt cctccttaaa actttctatt
     2761 ttaacgtttt gcagttaaaa acataaccat gcttcttcct tccacctttg agggcttatc
     2821 tactactgcg atatcagcca gactctcagc gaaatcgtcc agaatgtatt ttgacttgga
     2881 catatgtgcc atttcacgac cgcggaaacg aagagtaact tttactttgt ctcccttttc
     2941 caggaacttt ctggcagctc ctacttttgt gttcaggtcg ttagtatcaa tattcgggga
     3001 caaacgaact tcttttacat caattacttt ctgtttcttg cgggcttctt tttccttacg
     3061 agccagctcg taacggtatt ttccgtaatc gatgatctta cataccggtg gtttagcagt
     3121 tggtgcgatc tttaccagat ccagttctgc ttcctgtgcc agcttgaaag cgtctcttgc
     3181 agacataatt cctaactgtt caccattttc accgatcaat cttacttctc tgtctctgat
     3241 ctgttcgtta atcattaatt cgctaatagt tgtacacctc catagtcatg tttttgaatg
     3301 aagagcaaaa ataaagcgga tagccagtgc catccgcaaa aatacataat ctttcactga
     3361 aacctttatc tgtctctcaa tacgatacca gataaaaatc aaaccaaaac attcagtttt
     3421 cagtcagtgt ctgaaatatg aacccgggtc acttgctcgt gccagggtga ggcggatata
     3481 cctgctttcc tgtcagtcgt tctcacaact tttattacta tactctattt tattttcttt
     3541 gtcaataggt tttttattta tttttttctc cgttatccct gctcaaaagt accgcagcag
     3601 gtctctccgc agtcacaggc gccttctcca cggatatcga tctttcctgc acagcagcag
     3661 tgatcttcat tatatctgca gctgtccgca tcacaaacca catccagttt ggactcaggc
     3721 gttttaaaaa gatttttaaa gactccgcct gtgttttcag aaaaacttgc gcagcaggta
     3781 tcatctgcac agcgtgcatg agccccgtct actgtaatag aactcttaca gcagcaccct
     3841 tccgcatagt gtacacagtt ttttgcgcta cattccagtt tcgtcataat ctggtccctc
     3901 ctgatctttc attcaaacat ttcttaggtt taaaggttac cagactatta tgttccgaaa
     3961 ctcttttttt attctaagcg cgctttttta acggactttt gcgttcatgg cacgtactgc
     4021 ctggatacag gagctttctg ttccgtccat atggtacatg tccccctgcg taatgccagt
     4081 atacactttt acatcatcta tgtatactgt tcctgttccc tgggctgaaa tgaccagttc
     4141 tcctgcagat gcagtaccaa agttaagggt cacggtctgc ggactgcctg cagatacagt
     4201 ctgcttctgt cctcctgcct gcaccatgac ccggcagttt ccttctcctt ccagcagaaa
     4261 acttacatac aggttatttt cttccattcc tgcagagcgg ttgtggatat cctggctgat
     4321 ccatccggaa accggaagta ctgccatcct gcttccattg tgctcttcta ctttgctccc
     4381 gccggaaaat ttccagccct gctgtgcaag ggcaaactgg gaattataca ctttattatc
     4441 cccgtaatcc ctgtaggtcc agatcccgta tccaagtgtc tttgccttaa acagctctgc
     4501 cattccatcc agataagccg ctttctccgt ttccaaaagc tgggcattat gttcaaaacc
     4561 aggtgtatta tctgtaaata agaactggtc aatgtagatc tttttctgct ggctgctctg
     4621 gaataaccgg tcaaaataag aagctgcatt ggaaagagcc tgggctgctg ttactttttc
     4681 cccattattt tcaaatccca tgggaacgct gaacatggca ctggcaaagc cggatgagcc
     4741 acaggaaaaa gtagcatcat gaggagcacc ttctaatgtc ccgtccagcc gccttaccgg
     4801 atccacatcc agacgcactt ccatggaaag gtccggaaat acagtctggg tctctgcaag
     4861 gatatcattt aaaaaatggt cataaaactg gaaaaacaat tttcttgcat aagactcttt
     4921 tgccggcaga tagatttccc cataagagtc aaaggtatcc ctgtagatct ctcctaattc
     4981 ttctaatgta taatgttctt ttacatactg ggtatagccg cactgtgctg ccattttccg
     5041 tcctgtgatc ccgcttccat aaagggtact gccattttcc gcaaaattcc agaaatcttc
     5101 ccaggtaata aaaccgccgc tgaaattgtc ataagcagac gccttttcat acaaccgttt
     5161 tgcatagtga agccatgctt tctttgtttc actgtcataa agcagctttt cataacgggc
     5221 cagtacattt tcactgctgt aatagtccca ggtatatccc actcttaaca tgaccttcag
     5281 atcctgggct tttgccgcct tcattacccg gtcaaacttc tcccatgcgt agtcattata
     5341 gtgttccggc gccatagatg gctgaaattc cctccacggt actacaagaa taatgctgtt
     5401 aaacccatct tcccggatct gttttaactc ctcatccata tggctgcttt cactgttcca
     5461 gaaattgatc acccagtcat ccgagtaata ggttgcagac tttaaataac cattttcatg
     5521 gttcccatga tcagatgctt tcgtaaatcc ggtacagaac aaaaaagcag acgctgctgc
     5581 tataagaaca gcgcctgttg taacccttcg caaatttatc ttcatttgtt tttatccctt
     5641 tccccttttg taagttccgg ccatacggtg cagagcatgg tcacaaagca tgccgttccg
     5701 ccgataaagt tggacaccgg ttctgccata agaatgccgt ccgttcccat atgaaacatg
     5761 ctgggtaaaa taatgatcag cggtattaca atgatcactt tacggaaaat agagaagaat
     5821 accgcatttt tcgcttttcc aagaccaaca aagatggcct gtcctgcaaa ctgaaacgcc
     5881 atcatgaaaa agccgaagaa ataaatacgc atagccggaa ttcccgcttc caccagatcc
     5941 ccggtacggt tgaagatacg gatgaaaaac tccggaaatg catggaccag agaccacact
     6001 cctaatgtat acaaaacaga aacagcagat acaaacacaa ttgctttttt cacacggccg
     6061 tattcttttg caccgtaatt atatcccatc acaggctggg agctgtgggt aatgccctgg
     6121 accggcatgg aaatgacttc acggactgta ttaatgaccg tcataatacc tacatataaa
     6181 tcaccgcctg ttttctggag catggcatta tacatgacct ggacagaact gttggtaatg
     6241 gacatggtaa agccgctcat tccaaggcca atgatacgca ggaccctgga tcttttcagt
     6301 ttcatacagt tctttttcag ctttaaaata gcctttggac ctgtaaggaa ttttaagatc
     6361 cagatggcgg agatcagctg ggataagatc gttgccagcg ctgccccacg cacacccatg
     6421 tgaaagaaaa agataaaaac aggatccagg ataatatttg caactgctcc caataataca
     6481 gtcatcattc caatggtgcc aaaaccctgg gaattgataa agctgttcat tccaaggccg
     6541 gtcataacaa agatatttcc catcagataa atggtgatgt aggagtccgc atatccgatg
     6601 gttgcgtcac tggcgccaaa taaatacaac ataggccgtt taaatacaag tcccaccaca
     6661 gtcaggataa ctcctacaac aaccatcata gcaaaggaat ttcccataat ggcttctgcc
     6721 tcttcattgt ttccccgtcc tctttcaatg gaacacaatg gggcaccgcc cattccaaac
     6781 aggtttgcaa aggcgatcac aatggatatg atgggcagac acagtccaag acctgtcaaa
     6841 gatatggtcg cctcatcttt taactggccg atataaagcc ggtctacaat gttgtagagc
     6901 acattgatca gctgtgccaa tgtcataggc actgccagtt ttaatatgtt tacaacaatg
     6961 cttccctggg cgaagttatt cttctgttgt ttttccccgg cctcttcttt tacttcctct
     7021 tttacatttt ctttctgttt catttttctt cccttgtgct tttaaaacaa ttctgctgtc
     7081 tttaatatct gaaagatcac gtattttcca gttccaggcg caatgcagtc agatctgccg
     7141 gctggtcaat atcttttaat tctctttcat ccacttccat catccatatt tcctgtggat
     7201 atttctgcat gatctggcgt ccgccctgat ctccttccaa agcaagaagc tcttttttgt
     7261 agcgtctacg gaatatatta gggctgcctc tgtgtccctt tgcacacaca cagccgatcc
     7321 cttttttgct tttcataaac tcactgacaa acctggctat ggtctctcct gccatacagg
     7381 gctggtctgc cacaaaatac agatatgcat ctgcattatc ccaggcaacc tctgtgccaa
     7441 gtttaataga agctgtaatt ccctggtcgc tgtcctcatt aaaaaagcgc tctgcttccg
     7501 gaacctgctc cacagatccg gacagtaccc gttcatactg gctgatgagg atcagctgac
     7561 agttgatctt ttccgttttt tctaacaatt tcgctgcctt ttgcaagctt aaaagcccgt
     7621 gaagatacaa cggctcgcct ctgaattctg tgatcagttt gttggtccca taacgctttc
     7681 caaatccgga agccatatag atcattgata ccttcattgt acttacctct tttatctttt
     7741 tatacagtac atccccctaa aacaggttaa atgacgtttc cttccacagt tctcctgtta
     7801 atattccccg ttcttcctcc attctttcca gaatgttttt tccattttct tccagtttca
     7861 tggtatctgc ctggttaaag accgggatca cctgactgta aggaaattgt tgtctcaatg
     7921 gctctaaata cccctttttc attaaaagag acattttctc ctctgtcatg atccattttc
     7981 catctttttg atcctctggt atttttaaaa gctgttctga cggctgcatc ctgaaacagc
     8041 agtcggctgc ctgttttccc aaagctccaa gaccatagat acaaaggatc tgatcggaat
     8101 tttcaggggt caccggttct tttggtccag gcactttcac cggaagccgt ttggaaccat
     8161 ctgcctccac taagatcaca tctgccagtt ttcctgcttt ttcataaaac tcccagtccc
     8221 ggaaagcgat ctttccctct ttggcaggac atcctgcaac tgctatccct tctttttcca
     8281 gcattttttc tacttgtccc agatccggtt ccaatactcc aaaatattcc ggaactgcca
     8341 tatgggtcgt agtcattacc agcacacggc atcctttttc tctgccttcc caggccagac
     8401 ggcgtatata agaggttttt cccccgcctc ccacaaaaga aagaacaggg gaatggtctg
     8461 cctgacccgg atcttttttt aaccgatcgt ctataaaaag cgcggaccac aacgtatccg
     8521 tctgtgtcca ttttccctgt gtttttacag aaatcttcaa ataaattccc ccaaaatttg
     8581 tctttggagt aattttacca taaattctta ataccgtaaa gaaaacagct gtgtacaaat
     8641 ataaaacaaa tacaaaaaga cctgaaacag cgtctgttga tccagactgt ttcaggtctt
     8701 tgctggtttt caatttttat ctgttattat ttctcacaga ttactttata catatcgtaa
     8761 gctaaaattc caaatacaaa tgttactgcg attgctgcca tgttcttgtc ttcctttcct
     8821 gagtcgtagt ttttaattcg tagtttttat tgcttaaccg gtttgtttca acctgttgga
     8881 ttgctttctt aactctgtaa acagtataac ctaaaaattt tttaggtaca atccgcaaaa
     8941 ataataaggt ttttgagaat atctaaatat ttttgtatat tatgcaaaaa gttttagaat
     9001 taacaagccc gccaaattat taacatgttt ttgagaaaac ttatcattta atcctatgga
     9061 cattttccta taaatcatgt atgattttac caagagttag ttagcactaa ccattctgat
     9121 acgaaacata taacttacca ttcccccata catataatac aagaacatgt tctaaataca
     9181 cttttcaggg gcacacaaca cattatattg cacacaccaa aggaggctac attcatgagc
     9241 gttgttatca tcggcggaca tgaccgtatg gtcagccagt ataaaaaaat ctgtaaaaac
     9301 tataactgca aggcaaaggt attcactcag atgagtgcca gcctggacaa gcagatcgga
     9361 agaccggatc ttttagttct gttcaccaat accgtttccc acaagatgat ccgctgcgct
     9421 ttagacgagg tcaatgagca ggagaccgag atcgtgcgct gtcataccag cagcggtact
     9481 gctcttactg agatcttaat gcagcatgtt ccggcataga gacaggcatg aactaaaaaa
     9541 ataaaaacag gactgtaacc agaaattccc tggtccacag tcctgttttt tgtattttga
     9601 gatattttct tttgggtttt attccttttt agtactcttt caatgcccgt actgcacatt
     9661 ccacctgctc ttctgtatta aaatgagaaa aactgaaacg cacagccccc tggtccacag
     9721 tgcctaaagc cccatgcatt aaaggcgcac agtgaccgcc ggaacgggta tcgatcccat
     9781 attctgttga cagatagtcg ctgacttccc cggactcttc ttctcccagg tttaaagaca
     9841 caatagcagc tctgtctttc tgtgaaaaat ccccgtaaat gcggatatcc gggatctcct
     9901 ttacctgttc atagaaaagc tgcatcagct tctgttcccg ggcgcctatg gcctcgatcc
     9961 cttcttcttt tatgtaaaga agggcagctc tcagtccggc aatgccatgc ccgtttaaag
    10021 tacctgcttc taacgctgta ggcatcactg aaggatgctg tttttcaaag gtaaggatac
    10081 cgcttcctcc tgataataaa ggttttacag caagaccttt gcgcacacac agtccgcctg
    10141 ttccctgggg tcccataagg cctttgtgac cggtaaaaca cagcacatca atatggtttt
    10201 cttctatatt aataggaaaa tgacctgctg tctgggatgc atctacgata aaaagggctc
    10261 cggcctgtct gcaccactct cctacttttt ttagatctgt acgatttcct gtaagattag
    10321 atgcatgggt catgactact gccctggttc ccggtgtgat cgctgcctta agggctttgt
    10381 agtccagttt tccaaggcgt cttacccgtt ctttttcctc ttcaccggca ctttccggtg
    10441 cctgttcaca gccaacaatg accagttctg ctccctgttt ttccatttca tataaaggac
    10501 gcagcacact gttgtgttct aaaaccgttg tcactacccg gtctcccggt ctgatcagcc
    10561 ccttaatggc aatatttaag ctttcggtag aattggctgt aaatgccacc tgctccggac
    10621 catctccatg gaacatttct gataaaagga cccgggtctc aaaaatgact ctggctcctg
    10681 taagtgcttc tttataagct cctctggagc agtttcccat actggaaatg gcctctgcca
    10741 ccgcttctgc cacacagggc ggacggtaaa aagaagtagc cgcgctgtcc agatagatca
    10801 tatgctcccc tccttaatat ccctgctttt cctctaagta catcaggccc tctaaaacag
    10861 agcctccgat atttcttgcc ttatcagaaa tggtaaagca gtttttatat tcactttctc
    10921 tcgggtcgat atctgcgatc ttaaagccaa ctgttaccgg ataagcatca cggatcagac
    10981 cgcggaccag accggtaaga gttgcctcta ccttgacctc ttccccgttt tctgtgcaga
    11041 ttacagcaat ggtctgtcct ttttctacct tatcaccgat ctttactctg ccgtaaagct
    11101 ttccttctgc cggtgaatgg atgacccgtt cttttccgta tccgccgatg ataccgggag
    11161 tacctgtatt gggcgtagcc ggtccctttt cgataatacg gcccaggtta tggcctctct
    11221 gtgtttcaac tacaagatct acatctacac ctgcactaaa accaggtccc aaagcaatgg
    11281 tcttatctgc catctggcgg tttgtcccca gatttttctt tgccaaaatc gcatctacta
    11341 aggcccatgg acgtacctgt tttaagatat tgcactccgg atctactaaa agaggtactt
    11401 tctttttctc ttttaatacc tcttttgctt cttcccagga ctctaccttt atacaggtca
    11461 catcctcaac cttgctctcc ccgtcataaa cagcttctga aagcgccacc tgacggcgga
    11521 tcgcagacgg tgtggatatc tccaggatca ctaccggata tccgctgcgg aaaagccggc
    11581 tgatggtacc ggtagcaata tctccccctc ctcttacgat cacaagctgt tcatttctct
    11641 gtatcatata tttaccttct cctaaaaaat tatactttct aagtaaaaaa gaatgtgcct
    11701 tggcacaaaa tgccttatgg acgtaccact acgctggctt tggtcatttt ctccacgatc
    11761 tcatacatat tggtaacacc gcctacctga cgcttttcag ccagtccgta gtggtttaag
    11821 caggttccac aggtaaggat ctccacaccc tgtgcctcta aggtcttaag gtcttccagg
    11881 ttgtcagaac cttcacagga taagaaggca ccgctattgt ataaaagaat ggtctccggc
    11941 agcatatcct gctgggtcag tgcaaataca aagcccttca tcagaagctt tcctaatgcc
    12001 tcatccccct gtcccatctg gttggaggaa aggacaacta ccatgccttt tcttcttcca
    12061 tctggtgtgc aggtcacttc ttctgctgca actggtgctt cagcagcttc accatttcct
    12121 tctcctgcag taatggtaac taagaattca cgctcacctg tcttttcaga gcatacagca
    12181 agacctttgt gatctgccat tttacttaag ttctggactg cgatctcgtt atctacaaca
    12241 acttctacct tttctcctgg tgcagatgcc tctaatgctt tctttgcttc aataactggt
    12301 aatgggcact gttttcctct ttcatctaat ctcatggttt tcttcctctc tttccataaa
    12361 tctgatatcc aaaacctccg ttggcggttt attggcatgt atcttgtcaa gccgcccgtg
    12421 ggcggccatg ttcatctact ggatgataac cgccttctcc tgtttcggca ggatctctcc
    12481 tactacgccg cagggaagtc ccagtttttc cagttcttcc ataatagccg gaacatcttc
    12541 tttatctaca gatgccagca gtccgccgga cgtctgcgga tcatataaaa tctcttctgt
    12601 tgcaaaggag cagtttctaa actccacctt atcctgcaca tggtttctgt ttctctgggc
    12661 agcagctgtc aggtaaaatt cttctacata atcctcacat tccgggatat aaggcacttt
    12721 ttccttatag atccttgctg tagcctggtc tgtgaccatt tcacaaagat ggcctaaaaa
    12781 tccaaagcct gtcacatcgg tacagccgtg taaacggtat ttacgcagga tctcagacgc
    12841 atacttattt aatgtagtca tggatttcac tgccaggtca tatgcctctt tggaagctgc
    12901 tttcatacgt gatgccgtac atacgatccc gacgccaagt ggctttgtaa ggatcagcgc
    12961 atctcccact ttacagccgc tgttggcaaa cactttatcc ggatggatca cccctgttac
    13021 agacaggcca tattttacat cgctgtctgc aatggaatgt ccccctgcaa ggacgccgcc
    13081 tgcttctctt accttttcac tgcctcctaa catgatcttt cccaagatgt ttaaatccat
    13141 tgcttccggg aaacatacaa tatttaaagc tgtttttaca tcaccgccca tagcgtaaat
    13201 gtcacttaag gcatttgctg ctgcgatctg accgaaaata tagggatctt ccaccatagg
    13261 cgggaagaaa tccagtgtct gtacaatagc taggtcatcg gtaagcttgt acaccgctgc
    13321 atcatcagaa ccgtcaaagc cgataagcag gttgggatca taggtctttg gcaggttatt
    13381 taacactctt gccagtattc ccggtcccag cttggcagta cagccgcctc ctttacagaa
    13441 tacgatctcc tgtggctcca taatatcact ccattccttt tatcttttct accttttcct
    13501 ctgaaaaata ctgtgcaaac agctgatcca gatgatctac tgcatccttt ttaaagcctt
    13561 cgtatgcctc gatcagccgt tctgcctgtg gtgtcagtgt agaaccgccg ccgtcttttc
    13621 ctccggtctc acggtctgta agggcaaatc cccattcttt ttctgcccct tttaacatct
    13681 tccaggcttt gctgtaagcc atattcatag cctttgctgc tttttgcagc gaaccggtac
    13741 gctccacctc ccgaaggagc atgaccattc ccggtccaaa agctttttcc tcaaaataca
    13801 gcctcagcga cattccgtag tgaagcctgc tttcccccat atagtttcct ccgttattct
    13861 taattaaata caacgctata gcaaaaaaac cggcgggcca tttccgggat cttcatcccg
    13921 gagcaatggc cgtaccggct tttcacagtt tttctcccgg atcaccggac agttcctgca
    13981 tactgcttgc ataaagctcc aaactgcttt ttgtgttact tacttaagta taacattttt
    14041 ttgcaattat gcaaacattt ttagtttaat cttcttttct gatttcttct gcaacttcct
    14101 gggaaggaac atcctcttca aaggtacccc aaacgtgaaa ccctggtttc tgcaattaac
    14161 gcaggatcta tatcatccat aaagcaaaaa cagaaccatt gcaagctcat acctgcaatc
    14221 atcctg
//
LOCUS       DBMDUA010000061        14025 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2350_length_14025_cov_3.155559, whole genome shotgun sequence.
ACCESSION   DBMDUA010000061 DBMDUA010000000
VERSION     DBMDUA010000061.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 14025)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14025)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14025
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2350_length_14025_cov_3.155559"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /gene="rlmD"
                     /locus_tag="ACZPCL_06435"
     CDS             <1..953
                     /gene="rlmD"
                     /locus_tag="ACZPCL_06435"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1162138.1"
                     /translation="GSFYDIVNTPQCHIVHEDFRKILTATLEYFTEKGMGYYRKLQHE
                     GYLRHLLVRRAVKTGEILVALVTSGQTGKEGMPQSLETEKVLLEEWKKTLLALKMEGS
                     FAGILHIRNDTLADVVQSDETTVLYGKGYFYEELLGLKFQITPFSFFQTNSLGAEVLY
                     ETARGYVGETKDKVVFDLYSGTGTISQIIAPVAKKVVGVEIVEEAVGAAKENAEANGL
                     HNCEFIAGDVLKVIDEITEKPDLIILDPPRDGIHPKALDRIINFGVDHLVYISCKPTS
                     LVRDLVILQERGYKLERAVAVDMFPATANCETVAKLIRVK"
     gene            979..1455
                     /locus_tag="ACZPCL_06440"
     CDS             979..1455
                     /locus_tag="ACZPCL_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HIR1162139.1"
                     /translation="MKLLFRQRFFSWFDSYDIYDENENTVYTVKGQLSWGHCLKIYDV
                     AGREVGTVKERVFTFLPKFELYEGPQYIGCLSKEFSFLKPCYNIDFNGWEIQGSFMEW
                     DYTIATSDGRKVAVISKELFHMTDTYILDIADPKDALHVLMFVLAIDAEKCSRNRH"
     gene            1500..2807
                     /locus_tag="ACZPCL_06445"
     CDS             1500..2807
                     /locus_tag="ACZPCL_06445"
                     /inference="COORDINATES: protein motif:HMM:NF015515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HIR1162140.1"
                     /translation="MELAVLTLFAVLLIGALAAGVPILYALIAGLILFSAYSLKCGFT
                     LEQTGKMCLKGVNTVKNVLITMTIIGVLTGLWRSSGTIPAIVGYCVQLITPSVFLLLT
                     FLLNSLVSFLIGTSFGTAATMGAICIAMAQAMDISLALAGGAAISGAFFGDRCSPVST
                     STLLVAEVTHTDMYKNIPKLMKDSIVPFVISCVAYLVMGWLQSASGSAAAGAGELFSR
                     EFRMGLIPLIPAAAIFILAICKVEVKKAMAVSIFLAFVICLVYQHRPLTETLYSMVFG
                     YVSEDAQISKMLNGGGVQSMIKVVAVVCLSSCYSGIFEKTGLLLPLQEKIIKAGEKLA
                     PFAVVLPVSVIACAVSCNQSLATLVTEQLCNKLVTDQEKMAMYLEDGTIVIAPLIPWS
                     IAGAVPLASMGAPGTALFAGCYLYALPLWRLITDCFTRGKQKV"
     gene            2855..3052
                     /locus_tag="ACZPCL_06450"
     CDS             2855..3052
                     /locus_tag="ACZPCL_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1162141.1"
                     /translation="MAQDLTKGNPFSVILKFSLPVIGGNLFQLFYTLADTVIVGRTLG
                     ADALAAVGSTAIVIYLVLCFI"
     gene            3056..4186
                     /locus_tag="ACZPCL_06455"
     CDS             3056..4186
                     /locus_tag="ACZPCL_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1162142.1"
                     /translation="MTGGFGICLGHRFGARDEKGMRRSVAVSIILCVFFSLVIMAICC
                     GLAHQILYWQKTPEKIYDMAYDYMFAVLLGTGATIFYNMISNILRALGDSRTPLVFLV
                     FSSLLNIVLDVVFIVPFKMGVAGAAWATVLSQLLSAILCTVIGVRKFLILRLKKEDFQ
                     NLGSVIKTHMSMGFPMGFQMSVMCIGQLAMQGAVNALGTEAIAGYTAATKVDQFSVLM
                     NAAFGIAISNYVAQNYGAGLWDRIRKGTAACLLQTSIANALMCVMIIFGRHLVVPMFV
                     TQPTAEIIWYSDHYLLTVAPFYLLLGVLQIYRSAIQSMGNSWTPFTACIIELIFRITS
                     TTVFAGIIGYVGICFATPFAWIGASALLVPVYWHEILKRRKK"
     gene            complement(4235..5623)
                     /locus_tag="ACZPCL_06460"
     CDS             complement(4235..5623)
                     /locus_tag="ACZPCL_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1162143.1"
                     /translation="MIVHIIEAVYHFLWGDLVTLHLSGTSIGIPLLVLLLIPTGIYFT
                     FRTNFVSVRLFPDMVRSLTEKKGNGNSLSPLQTLFVSTATRVGMGNLVGVVAAVSAGG
                     AGAVFWMWLSAILGSATAFIEATLAQIHKEEDSLYGGYHGGPAYYIHYYAEKVRGKKL
                     NHSVIAVFFALSGLLCWCGISQVISNSVASAFENAFSVPPIITTVILVILSAIIVLRK
                     NATIKALDIIVPVMAAMYFIITIFIIVTHIGQLPGVFSRIFKEAFGLRAVAGGGFGAV
                     LMNGVKRGLFSNEAGSGSAPCAAAAADEKEPVKMGLIQALGVFIDTIVICSCTAFILL
                     LVPEELTRGLMGMELLQTAMNYHLGSFGVIFVAVILALFSFSTFLGILFYARSNVSYL
                     FGNRWLWQTLYKILALIMLFIGGIQAYTVVWDLGDVGIGLMTIFNLAVLFPLSNEALY
                     ALAEYEKQKKCE"
     gene            complement(5759..>5966)
                     /locus_tag="ACZPCL_06465"
     CDS             complement(5759..>5966)
                     /locus_tag="ACZPCL_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162144.1"
                     /translation="SERATGCFHLMPQSACAREQKKGKMAVLPIENLSMMRETKIVYK
                     KDFTHMDILQEITRVYHETARSYQ"
     assembly_gap    5967..5976
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5977..6740)
                     /locus_tag="ACZPCL_06470"
     CDS             complement(<5977..6740)
                     /locus_tag="ACZPCL_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1162145.1"
                     /translation="MLGSKIETLLAVAEFQNFTKAAASLSLTQPAVSHHINQLEEELG
                     VTLFIRGKGSLMLTPEGEIVLKYARRIKALYGKMETEIDNSRKQLTKVRIGITHTSES
                     NLITEVLARCSNADNNFSITIITDTINNLYDMLDNYEIDLAVVDGTPTGHSLYSLMLA
                     TDYLVCVMSIRHPLAKRAMVTLSELKQERMILRLPTSDTRMLFESTLKSIGDSIDNFN
                     ITLEVDNIATIKDLVRKGLGVSILPQNATEEQKKGE"
     gene            6866..8305
                     /locus_tag="ACZPCL_06475"
     CDS             6866..8305
                     /locus_tag="ACZPCL_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR1162146.1"
                     /translation="MKSFLFYHATSRASVVIITIALMLFVGYLMTRITRKFKLPNVTG
                     YILAGILMGPYCLDMVPVKVIDGMDFLSDIALAFIAFSTGEFFRFSVLKKNGWKVIVI
                     TVMEAVLASVCVFAVVYGVLHLNLAFSIVLAALASATAPASTIMTIRQTGAKGDFVDT
                     LLQVVALDDVVGLVEYSAAISIALAALSGTGGFQVGNILKPILINGAVLVLGGIFGWL
                     LKCMMPKKKYKDNRLIISVAALFAFCGICSLADVSPLLGCMSMGTIYINLSDDDKLFK
                     QLNYFSPPILLLFFVRSGLSFRLDALLNPSAGEDGASLLVIGVAYFLTRIIGKYAGAY
                     LGCLAVHKKKQVRNYLGLALVPQAGVAIGLAALGARTLGGQMGIDLQTVILASSVLYE
                     LTGPACAKLSLYLSGSYGDIELPKEAKEKDPVTRLEMLIERIHEIQQEIPETEENISP
                     EEQAFLEAANEQYEAVGALRNHLRARRTY"
     gene            8307..9023
                     /locus_tag="ACZPCL_06480"
     CDS             8307..9023
                     /locus_tag="ACZPCL_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014373.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR1162147.1"
                     /translation="MGHITAELTKGWMMEGIGMQVILRILLSLALAAIIGCERSSKRH
                     SAGMRTFMLVSVSATAAVFTDLYLSSEFSFGIPVISGAAVVGIAMLSGNSILFSSKNQ
                     IKGLTTSAALWACGFMGLMVGAGMYISAFVIFMALLCGISLFPSAERYLKDRSNHFEV
                     HLELKSSFKLQDFVATIRKLGIRIDDIEANPAYLNSGLSVYTVSLTVDSQELKKDKKH
                     SDIIEALRSLDYIYYIEEIK"
     gene            9087..9175
                     /locus_tag="ACZPCL_06485"
     tRNA            9087..9175
                     /locus_tag="ACZPCL_06485"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9121..9123,aa:Ser,seq:cga)
     gene            complement(9370..10257)
                     /gene="rsiV"
                     /locus_tag="ACZPCL_06490"
     CDS             complement(9370..10257)
                     /gene="rsiV"
                     /locus_tag="ACZPCL_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-V factor RsiV"
                     /protein_id="HIR1162148.1"
                     /translation="MKEQDRNLEYFDDLDKLESLKQEYNNIPVPEAAKARIMSGIQKG
                     KTMNKKHNPFFRILRTTGTTAAAAVVALAIVTNVSPTIANAMTNLPVIGAIAKVVTLR
                     TYEDKTNHFEAKVDIPEIDSAPEAVNRSIEDYANELIAQYEKDLRASQGEGNYSLTST
                     YKVVTDTDRYLCLRIDTTLVMASGTEYTKVFTIDKTTGDIISLSDLFKNKPEMLTAIS
                     DNIKEQMKQQMAADSSVTYFIDSDMPEWDFKELKGDESFYFNEKGELVITFDETEVAP
                     AYMGAVEFTIPQSVTGDLK"
     gene            complement(10277..10774)
                     /locus_tag="ACZPCL_06495"
     CDS             complement(10277..10774)
                     /locus_tag="ACZPCL_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1162149.1"
                     /translation="MNHSDQTKQITAQTEKMLIDQYERLYRLAYSYVHNEADALDIVQ
                     ESACKAITQCKNLKDTEKLFPWLCRIVVNTSLDLLRSRTKEQPAEELPETATEDTYEE
                     FDLKEALNHLEPKNRTIIILRYFEDMKLEDIALAVAENVNTVKARLYRSLKKLRIHLD
                     GTASF"
     gene            10985..11608
                     /locus_tag="ACZPCL_06500"
     CDS             10985..11608
                     /locus_tag="ACZPCL_06500"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HIR1162150.1"
                     /translation="MAAVFMAMMIGILTGLGSGYCIWGREPENTIDLKAVEMPDWVQQ
                     DFLRKNIFSRPDVTRRQVKNIVIHYVGNPGTSAKATRNYFDGLADQDAQKEGVSSSSH
                     FVVGLDGEVIQCIPIDEVAYANAPRNDDTLSIEVCHPDDTGKFNDASYESVVKLTAWL
                     CKQLKLKSSDVVRHYDINGKQCPKYYVEDKEAWKQLREDVKEQMKAD"
     gene            complement(11683..12498)
                     /locus_tag="ACZPCL_06505"
     CDS             complement(11683..12498)
                     /locus_tag="ACZPCL_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HIR1162151.1"
                     /translation="MDAIYDCHLHTEFSADSETPVRDQLEKAISLGMKEICITDHHDY
                     GSEFCPDDFTLDIPSYIESLRKIRDEYAGRIRLNIGIELGLQNHVGEYLHDFARTLGK
                     EFDFVLGSSHFVDRMDPYEREYWKHFEEKEGFRAFFEVSLKRVQNLLDTFDSYGHLDY
                     VVRYAPHKNEFYSWNDYEPYIRPLLKLLIENGKCLECNTGGIRYGLGNTNPCADVFRL
                     YKELGGEGITIGSDAHTPETLGIGFDFCREMLKEYGFRYYTVFHERKAEMLPL"
     gene            12812..13843
                     /gene="gap"
                     /locus_tag="ACZPCL_06510"
     CDS             12812..13843
                     /gene="gap"
                     /locus_tag="ACZPCL_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR1162152.1"
                     /translation="MAVKVAINGFGRIGRLAFRQMFGAEGYEVVAINDLTDPKMLAHL
                     LKYDTAQGGYAGRIGEGKHTVEAGEDSITVDGKKITIYKEPKAADLPWGELGVDVVLE
                     CTGFYTSKEKAQAHIDAGAKKVVISAPAGKDLKTIVYSVNEKTLTAEDTIISAASCTT
                     NCLAPMAKALNDYAPIQSGIMSTIHAYTGDQMILDGPHRKGDLRRARAGAANIVPNST
                     GAAKAIGLVIPELNGKLIGSAQRVPVPTGSTTILTAVVKKADVTAEEINAAMKAAASE
                     SFGYNEDPIVSSDVIGMRYGSLFDATQTMVSHIADDLYEVQVVSWYDNENSYTSQMVR
                     TIKYFAELA"
BASE COUNT         3818 a         2859 c         3389 g         3949 t
ORIGIN      
        1 gaggcagttt ttatgacatt gtaaataccc cgcagtgtca tattgtccat gaggatttcc
       61 gtaaaatatt aacagctacc ttagagtatt ttacagaaaa aggcatgggc tattacagaa
      121 agctgcagca tgagggctat ttaagacatt tgctggtgcg tagagctgtt aagacaggtg
      181 agatcctggt agctttggtt acttccggac agaccggcaa agaaggaatg ccacagtcct
      241 tagaaacaga aaaagtgtta ttggaagagt ggaagaaaac actgttagct cttaaaatgg
      301 agggaagttt tgcaggtatc cttcatatcc gcaatgatac tctggcagat gtggtacaga
      361 gcgatgaaac tacagttctt tatggaaaag gctatttcta tgaagaattg ttgggcctga
      421 aattccagat caccccattt tccttcttcc agaccaattc tctgggagca gaggtgcttt
      481 atgagacagc cagaggttat gtaggagaaa caaaggataa agtagttttc gacttataca
      541 gcggaaccgg aaccatttcc cagatcattg caccggtagc aaagaaggta gtaggggtgg
      601 agatcgtaga agaggcagta ggcgcagcaa aggaaaatgc agaagcaaat ggtctgcata
      661 actgcgaatt tattgcagga gatgtattaa aggtgatcga tgagataacc gaaaagccag
      721 acctgatcat cttagatccg ccaagagacg gcatccatcc aaaggcatta gaccgtatca
      781 ttaattttgg tgtggatcat ctggtctaca tttcctgtaa gccaaccagc ctggtaagag
      841 atctggtgat tttacaggag agaggttata agctggaaag ggcagtagct gtagacatgt
      901 tcccggcaac tgctaactgt gaaaccgtag caaaactgat ccgtgtaaaa taatccggtt
      961 ataaaagggg gttacattat gaaacttctg ttccgtcaac gcttcttttc ctggtttgac
     1021 agctatgata tttatgatga aaatgagaat actgtataca cagtgaaagg tcagctttcc
     1081 tggggacact gcctgaagat atatgatgtg gccggccgtg aagtggggac agtgaaagag
     1141 cgggtattta cttttctgcc aaagtttgag ctgtatgaag gtccgcagta cataggctgc
     1201 ctcagcaagg aattttcgtt cttaaagccg tgttacaata ttgattttaa cggctgggag
     1261 atccagggaa gttttatgga gtgggactat acgatcgcga cgtctgatgg gcggaaagtg
     1321 gcagtgatct ccaaggaact gttccatatg acggatacct atatactgga tattgcggat
     1381 ccgaaagatg cccttcatgt gctgatgttt gtgctggcca ttgatgcgga gaaatgttcc
     1441 agaaacaggc attaaaagcg gtttagaata aaattacaga agataaaacg aggtgtagaa
     1501 tggaattggc ggtgctgaca ctgtttgcag tgcttttgat cggtgcgctg gcggcaggtg
     1561 tccctatttt gtatgccctg atagcaggtc tgatcctgtt ttcggcatac agcttgaaat
     1621 gtggatttac cctggaacag acggggaaaa tgtgtttaaa aggcgtaaat acagtaaaaa
     1681 atgtgctgat cactatgacg atcatcggag ttcttacagg gttatggaga tcatcaggaa
     1741 cgatcccggc tattgttggc tattgtgtgc agctgatcac accttccgta tttttgctgc
     1801 ttaccttttt gttaaacagc ctggtatctt ttctgattgg aacttccttt ggaacggcag
     1861 ccactatggg agccatctgt attgccatgg cccaggcaat ggatataagc cttgcccttg
     1921 caggcggcgc agctatatcc ggcgcctttt tcggggaccg gtgttcgcct gtatccacca
     1981 gcactctgct tgtggcagaa gtgacccata cggatatgta taaaaatata ccgaaactga
     2041 tgaaagattc tatcgttcct tttgtgatat cctgtgtggc atacctggta atgggatggc
     2101 tgcagtctgc ttccggcagt gcggcagcag gagcagggga gctgttttca agagaattcc
     2161 gtatgggact gatccctctg atcccggcag cagccatttt tattttagca atatgcaaag
     2221 tagaagtgaa aaaggctatg gcagttagta tttttctggc atttgtgatc tgtctggtct
     2281 accagcatcg tcctcttacg gaaactttat actctatggt atttggctat gtttcagagg
     2341 atgcccagat cagtaaaatg ctaaatgggg gcggcgtcca gtccatgata aaagtggtag
     2401 ctgtagtgtg cctgtcttcc tgttattccg gtatttttga gaaaacaggg cttttgctgc
     2461 ctttacagga aaagatcata aaagcaggag aaaagctggc gccttttgcc gttgtacttc
     2521 cggtttctgt gatcgcctgt gcagtgtcct gcaaccagtc gctggctaca ctggtaacag
     2581 agcagctttg taataagctg gtgacagatc aggagaaaat ggccatgtat ctggaagacg
     2641 gtaccattgt gatcgcaccg ctgattccat ggtccattgc aggcgctgtt cctttagctt
     2701 ctatgggagc acctgggact gcgctgtttg cagggtgtta tctgtatgcc cttcctttat
     2761 ggaggctgat cacagactgt tttacacggg gaaaacagaa ggtataaagt gtattggttt
     2821 gattggggaa aaacatgaaa aggaagtaaa agtcatggca caggatttga caaagggaaa
     2881 tccattttcc gtaatcttaa aattttcact gccggttatc ggtggtaatc tgtttcagtt
     2941 attttatact ctggcagata cagtgatcgt aggcaggacg ctgggagcag acgctttggc
     3001 agcagttggt tctacggcga ttgttattta tctggtactc tgttttatct agggtattac
     3061 cggcggtttt ggtatctgtc tgggacacag gtttggcgca agagatgaaa aaggaatgcg
     3121 tcgcagtgtg gcagtgtcta ttattctatg tgtttttttc tcactggtta tcatggcgat
     3181 ctgctgcggc ctggctcatc agatcctgta ctggcagaaa acaccggaaa agatctacga
     3241 tatggcttat gattatatgt ttgctgtatt gttaggaacc ggtgcgacta tcttttataa
     3301 catgatatcc aatattcttc gtgcattggg agacagccgt acacccctgg ttttccttgt
     3361 attttcctct cttttaaaca ttgtcctgga tgtagtattt attgtcccgt ttaaaatggg
     3421 agtagcagga gctgcctggg caacggtact cagccagctt ttatcagcaa tattatgtac
     3481 agtgataggc gtgagaaaat ttctcatact cagactgaag aaagaagatt ttcaaaatct
     3541 tggttctgtg ataaaaactc acatgagtat gggatttcct atgggatttc agatgtctgt
     3601 tatgtgcatc ggccagcttg ccatgcaggg agccgttaat gcactgggaa cagaggcaat
     3661 tgccggatat acggcagcga cgaaagtgga tcagttttct gtacttatga atgcagcttt
     3721 tggtatcgcc atctccaact atgtggccca gaactatggt gccggtctgt gggatcgtat
     3781 ccgcaaggga acggcagcct gcctgcttca gacaagcatt gcaaatgctc ttatgtgtgt
     3841 gatgatcatt ttcggacgcc atctggtcgt gcccatgttt gtaacacagc ccacagcaga
     3901 gatcatctgg tattctgatc attatctgct gaccgtagcg cctttttatc tgcttcttgg
     3961 agtgctgcag atctatcgtt ctgccatcca gagtatgggc aacagctgga caccctttac
     4021 agcctgtatc attgaactga tctttcgtat tacatccacc actgtgtttg cagggatcat
     4081 cggttatgta gggatctgct ttgcaacgcc ctttgcatgg atcggggcga gcgcattgtt
     4141 agtgcctgtg tactggcatg aaatattaaa aagaagaaaa aagtaaattc ggaaaagaaa
     4201 aggacagagg cgatcaacct tctgtctttt ttagctactc acattttttc tgtttctcat
     4261 attcagccag cgcatataag gcctcatttg aaagaggaaa gagcactgcc agattaaaaa
     4321 tagtcatcag tccgatgcca acatctccca gatcccacac cactgtataa gcctggatgc
     4381 cgccgatgaa gagcatgatc agggccagga tcttatagag agtctgccac agccagcggt
     4441 ttccgaaaag ataggacaca ttggaacggg cataaaagag gataccaagg aaagtggaga
     4501 aactgaacag ggccaggatc acagctacga agatcacacc aaagcttccc aggtggtagt
     4561 tcatggcagt ctgtaacagc tccattccca taaggccacg ggtcagttct tccgggacaa
     4621 ggagcaggat aaaggcagta cagctgcaga tcacaatggt atcaataaat acgcctaaag
     4681 cctggatcag tcccatttta accggttcct tttcatcagc agcagctgcc gcgcagggag
     4741 cagaaccgga gccagcctcg ttggagaaaa gaccacgttt tacaccattc atcagtacag
     4801 ctccaaaacc accgcctgct actgcacgaa gtccgaaggc ttctttaaaa atcctggaaa
     4861 atacgcctgg aagctgtccg atatgggtca caatgataaa aatcgtgatg ataaaataca
     4921 tagctgccat aacaggaaca atgatatcca gtgcctttat ggtggcattt ttacgcagca
     4981 cgataatggc ggataagatc accaggatca cagtggtgat gataggtggt acagaaaaag
     5041 cattttcaaa ggcagaggca acggagttgc tgatcacctg gctgatgccg caccagcata
     5101 agagtccgga taaggcgaag aaaactgcga tcacagagtg gttcagtttc tttccacgga
     5161 ctttttctgc ataatagtgg atatagtaag ccggaccgcc gtgatagccg ccgtacaatg
     5221 aatcttcctc tttatggatc tgggccagag ttgcctcgat aaaagcagtg gcagagccta
     5281 aaatagcgga aagccacatc cagaacacag cacctgcgcc gcctgcagaa acagcagcca
     5341 ctacgcccac cagatttccc ataccaaccc gggtagcggt agatacgaaa agagtctgta
     5401 acggtgataa gctgttgcca tttcctttct tttctgtcag ggaacgcacc atatccggaa
     5461 aaagacgcac ggatacaaag ttagtacgga aggtaaaata aatacctgtt gggatcagaa
     5521 gcagtaccag aagaggaata ccaatggaag tgcctgaaag gtgcagtgta accagatctc
     5581 cccacaaaaa gtggtaaaca gcttcgatta tatgaacgat cataaattat ctccagtcat
     5641 tttcattttt ggacctgtta aaacgttgaa acacacttaa acgctttaaa gggccagtct
     5701 aattattgta cactgttttt gatgatttgt aaaactataa aacagtggaa aatgtctatt
     5761 attggtaact tcttgccgtt tcatggtata cccgggtgat ctcctgaagg atatccatat
     5821 gggtaaagtc ttttttgtat acgatcttgg tctcacgcat catacttaag ttttcaatag
     5881 gaagaacggc catttttcct tttttctgtt ctcttgcgca ggcactctga ggcatgagat
     5941 ggaaacaccc cgtggctctc tctgaannnn nnnnnncact cgcctttttt ctgttcttca
     6001 gtggcattct gaggcaggat ggaaacgccc agtccttttc gtaccaggtc ttttatagtg
     6061 gcgatattgt caacttccag agtgatgttg aaattgtcaa tggagtcccc gatgcttttt
     6121 aatgtggact caaaaagcat gcgtgtatca gaagtaggaa gccgcaggat catccgttcc
     6181 tgttttaatt ctgataaagt gaccatggca cgtttggcca gaggatgccg gatggacata
     6241 acacacacca gataatcggt agctaacatc agggaataga gggaatgccc ggttggcgtt
     6301 ccgtccacaa cagccagatc gatctcgtaa ttatccaaca tatcataaag attatttatg
     6361 gtatccgtaa taattgttat tgaaaaatta ttatcagcat tgctgcaacg ggccagaacc
     6421 tccgtgatca ggttgctttc cgaggtgtgg gtgatgccga tgcggacttt ggtcagctgt
     6481 tttctggaat tatcgatctc cgtctccatc tttccataca gcgctttgat ccttcgggca
     6541 tattttaaga cgatctcacc ttccggggtc agcattaaac tgccttttcc gcggataaaa
     6601 agggtgactc ccagttcttc ttccagctga ttgatatgat ggctgactgc cggctgggta
     6661 agggagagag aagcagccgc ttttgtaaaa ttctggaatt cagctactgc cagcagagtt
     6721 tctatttttg aaccgagcat ggtacctcct tattgtgtat aataaataaa atttatcata
     6781 cataaaaata aaataatttg cttttattat aaaacagtat atcatataga agcatgacac
     6841 acaaggaaaa cgagggggtt atagcatgaa gtcattttta ttttaccatg caacaagccg
     6901 ggcatcggtg gtcattatca ccattgcact gatgctgttt gttggttatc ttatgacccg
     6961 catcaccaga aagtttaaac tgccaaatgt aacggggtac attctggcag ggatccttat
     7021 gggaccctac tgtctggata tggtgccggt gaaggtcata gatgggatgg attttctttc
     7081 agatatcgcg cttgctttta ttgcattcag cacaggggaa tttttccgct tttccgtgtt
     7141 aaagaaaaac ggctggaagg tgatcgtgat cacggttatg gaagcggtgc tggcatcggt
     7201 atgtgtattt gcagtggttt atggggtgct ccacttaaat ctggcatttt ccattgtact
     7261 ggcagctctg gcatcagcta cagctcctgc gtccaccatc atgaccatcc gtcagaccgg
     7321 agcgaaggga gattttgttg atactctttt gcaggtggtg gcattggatg atgtggttgg
     7381 tctggtggag tacagtgcgg ctatttctat tgcgctggca gctctttccg ggactggtgg
     7441 tttccaggtg ggaaatatct taaaaccgat cctgatcaat ggggcagtac tggtgttagg
     7501 cggtattttt ggctggctgt taaaatgcat gatgccaaag aaaaaataca aagacaaccg
     7561 gctgatcata tcggtggcag cgctgtttgc tttttgcggg atctgttctc tggcagatgt
     7621 atcccctctt ctgggatgta tgtccatggg aaccatttat attaatttat ctgatgatga
     7681 taagctgttt aaacagttaa attatttcag tccgccgatc ctgcttttgt tttttgtacg
     7741 gtccggactt tccttccggt tagatgcgct tcttaatccc tcagcaggag aagacggggc
     7801 ttccctttta gtgatcggag tggcatattt cctgacacgt atcataggaa aatacgcagg
     7861 tgcttattta ggctgtcttg cagtacataa gaaaaaacag gtgcgcaatt atctggggct
     7921 ggcactggtg cctcaggcag gcgtagccat tggtctggcg gctctgggag caagaacttt
     7981 aggcggtcag atggggattg atctgcagac cgttattctt gcatctagtg tactttatga
     8041 actgacgggt ccggcatgcg caaaattgtc cctgtatctg tccggatcct atggggatat
     8101 tgaactgcca aaagaggcga aagaaaaaga tccggtgacc cggttggaaa tgctgatcga
     8161 gcggatccat gagatccagc aggaaatacc ggaaacagaa gagaatattt ctccggaaga
     8221 acaggcattt ttggaagctg ccaatgagca gtacgaagca gtgggggcgc tgcgcaatca
     8281 tttaagagca aggaggacgt attgatatgg gacatataac agcagaactg acaaagggat
     8341 ggatgatgga aggaatagga atgcaggtga tcctgcggat cctgttatca ctggcgctgg
     8401 cggcgatcat tggatgtgaa cgctccagca agcgtcattc cgcaggtatg aggactttta
     8461 tgctggtcag tgtatctgct acagcagccg tgttcacaga tctgtatctg tccagtgaat
     8521 tttcttttgg gatccctgtt atttcaggag cggccgttgt gggcattgcc atgttaagcg
     8581 gcaactccat tcttttcagt tccaagaacc agatcaaggg actgactaca tctgcagcac
     8641 tctgggcctg cggttttatg ggcttaatgg ttggggccgg aatgtatatt tccgcatttg
     8701 tgatctttat ggcacttctt tgtgggattt ccctgttccc atctgcagag cgttatttaa
     8761 aagaccgttc caatcatttt gaggtgcatc tggaattaaa gagcagtttc aaacttcagg
     8821 attttgtagc gacgatccgt aaactgggga tccgcattga tgatattgag gcaaatccgg
     8881 cttatttaaa ttccggtctc agcgtgtata cggtttctct gactgtggac agtcaggaac
     8941 tgaaaaaaga taaaaaacac agtgacatca ttgaagcact gcgctctctt gattacattt
     9001 actatataga agaaatcaaa taaaaacgat ttgcctacta cccaaagcag gaaaagtatt
     9061 gtataattaa caggttagca ataacaggag gaatacccaa gtggttgaag ggtctggctt
     9121 cgaacaccag taggtcggta gcaccggcgc aagggttcaa atcccttttc ctccgtaaat
     9181 aagaatgtgc cttggcacat tctcgcgccg agcgcggtcg cttgcgacaa acttcctcat
     9241 cgcgcgagtg cgcatcgttg gcaggtcgtg cctttttgtg tactagggag ttccaacgga
     9301 atttcctagt acataagaaa gcagccgcag gaaatgcacc tttgatgtgt tcctgcggct
     9361 gttttgctat tatttcagat caccggttac agactgggga atggtaaact ccacagctcc
     9421 catataagcc ggggctacct cggtctcgtc aaaagtaatg accagttctc ctttttcatt
     9481 aaaatagaag ctttcatcac cttttaattc cttaaaatcc cattccggca tgtcactgtc
     9541 aataaaataa gtaacagagg agtctgcagc catctgctgt ttcatctgtt cttttatgtt
     9601 gtcgctgatg gcagtgagca tttccggttt gtttttaaat aaatcagaaa gagaaataat
     9661 atcaccagtg gtcttatcaa tggtaaatac cttggtgtat tcggttccgc ttgccatcac
     9721 aagggtggtg tcaatgcgca ggcacaggta tctgtctgtg tcagttacta ccttgtaagt
     9781 agaagtgaga gagtagttgc cttctccctg ggatgcccga agatcctttt cgtactgggc
     9841 gatcagttca tttgcgtagt cttcaatgga gcggtttaca gcttccggtg cagagtctat
     9901 ttccggaata tccactttgg cttcaaaatg attggtctta tcttcgtatg tacgtaaagt
     9961 gaccactttt gcaatggcac cgatcaccgg caggtttgtc atggcattgg caatggttgg
    10021 gcttacgttg gtgacaatgg ccagagctac tacagcagcg gcagcagtag tgccggtggt
    10081 gcgtaaaata cggaaaaatg ggttgtgttt tttattcatg gtttttcctt tctggatacc
    10141 tgacatgata cgtgcttttg cggcttctgg aacgggaatg ttgttatatt cctgctttaa
    10201 agactccagt ttatctaaat catcaaaata ttctaaatta cgatcctgtt ctttcattaa
    10261 tatatgctcc tttcttttaa aaactggcag tgccgtctaa atggatccga agctttttta
    10321 acgaccggta taatcttgct tttacggtgt ttacattttc ggctaccgca agggcaatgt
    10381 cctctaattt catatcttca aaatagcgaa ggatgatgat ggtgcggttt ttcggttcca
    10441 aatggttcag tgcttctttt aagtcaaatt cctcatatgt atcttctgtt gccgtttcag
    10501 gaagttcctc ggcaggctgc tcttttgtcc ggcttcgcag caggtccagg cttgtattta
    10561 ctacgatccg gcacagccat ggaaaaagtt tttctgtatc ctttaaattt ttacactgtg
    10621 taatggcttt acaggcactt tcctggacga tatccaaagc gtctgcttcg ttatgtacat
    10681 aactgtaggc caggcggtaa aggcgctcat attggtcaat gagcattttt tccgtctgcg
    10741 cggttatttg ttttgtctga tcagaatggt tcatgatatg ttctcctttc atgtcttaca
    10801 cttattagac gcatgaaccg ggaaaaaagt tgcaaaagat attataatct tcataaaatt
    10861 tactgcaagt ccttaagaag ttcttcattg agtttttcag ggaataatgt tatattggaa
    10921 aaaagataag atacagggga tagataactt ggaaaagcga gaacgaaagg tgataacctt
    10981 tcctatggcg gccgttttta tggcaatgat gatcggtatt ctcaccggtc ttggaagcgg
    11041 ctattgtata tggggaagag agcctgaaaa tacaattgat ctgaaagcag tggaaatgcc
    11101 tgactgggta cagcaggatt ttttgcggaa aaatattttt tcccgcccgg atgtgacccg
    11161 caggcaggtg aaaaatattg tgatccatta tgtgggaaat ccgggaacca gtgcaaaggc
    11221 taccagaaac tattttgacg gtctggcaga ccaggatgca cagaaagagg gggtttcttc
    11281 cagcagtcat tttgtagtgg gattggacgg ggaagtgatc cagtgcattc ccattgatga
    11341 ggtggcatat gccaatgctc ccagaaatga cgatacgctt tccattgaag tctgtcatcc
    11401 ggatgataca ggcaagttta atgacgcttc ttatgagtca gtggtgaaat tgacagcatg
    11461 gctctgcaaa cagttaaaac ttaaaagttc cgatgtggtc cgtcattatg atattaatgg
    11521 aaaacagtgc ccgaagtatt atgtagaaga taaagaagcc tggaaacagt taagagaaga
    11581 tgtaaaagag cagatgaagg cagattaatg gagccgtctt gcaaatgtca ttggcacgat
    11641 acatggatct gtaaaaaact ctgtaaaaaa gcggagacgt ccctataatg ggagcatctc
    11701 cgcttttctt tcgtggaata ctgtataata gcggaaacca tattctttta acatttcccg
    11761 gcagaagtca aagccgatgc ctaaagtttc cggcgtatgg gcgtcagagc caatggtgat
    11821 cccttcaccg cccagttctt tatagagacg gaaaacatca gcacatggat tggtgtttcc
    11881 aagtccatag cggatgccgc ctgtgttaca ttccaggcat tttccatttt caataagaag
    11941 ttttaaaaga ggacggatat agggttcgta atcattccag gaataaaatt catttttatg
    12001 gggagcatat cttacaacat agtccaggtg gccataggag tcgaaagtat caaggaggtt
    12061 ttgtacccgc ttaagggata cttcaaaaaa tgcacgaaat ccttcttttt cctcgaaatg
    12121 tttccagtat tccctttcat atggatccat ccggtctacg aaatggctgg aaccaaggac
    12181 aaaatcaaat tcctttccca aggttctggc aaaatcgtgg agatactcac ctacatggtt
    12241 ttgtaatcct aattcgatcc ctatatttaa acggatccgt ccagcgtatt cgtcccgtat
    12301 tttgcgtaaa ctttctatat aagaaggaat gtctaacgta aaatcgtcag gacagaattc
    12361 gctgccgtaa tcatgatgat cggtaataca gatctctttc attccaaggg agatggcttt
    12421 ttctaactga tcccgtaccg gggtttcgga gtctgcggaa aattctgtgt ggagatggca
    12481 gtcataaatg gcatccatag tgatatgcct cctttttatc atattgcgtt tgtatccata
    12541 aaagcacata tctgccccac ggggagtgcg gcagctgtat tcgtctctca cattatactc
    12601 ataaactgcc acaataacaa gtctatgccc cacccttcta ttgagaaaat aaaaatgcgc
    12661 tgaaaattaa tggaatttat gagagaaaat agtcagaatg gcagaaacaa aaaaaaggct
    12721 tgctattttg gataaaattg agtacaatgt aggcagttga ttaatattta acaaataata
    12781 ttttttttaa caaactagga ggaattgaat catggcagta aaagttgcaa ttaatggttt
    12841 cggacgtatt ggacgtctgg ctttccgtca gatgttcgga gcagagggtt acgaggtagt
    12901 agcaatcaac gacttaaccg atcctaagat gttagcacat ctgttaaagt atgatacagc
    12961 acagggtgga tacgctggac gcattggcga aggcaagcac actgtagagg ctggagaaga
    13021 ttccatcaca gttgacggca agaagatcac tatctacaaa gagccaaagg ctgctgacct
    13081 tccatggggc gaacttggcg tagatgtagt attagagtgc actggcttct atacttccaa
    13141 ggaaaaagca caggctcata tcgatgcagg cgctaagaag gttgttattt ccgcaccagc
    13201 tggaaaagat ttaaagacca tcgtttactc tgtaaatgag aagactctga cagcagaaga
    13261 taccatcatc tccgctgcat cttgcaccac caactgctta gcacctatgg ctaaggcatt
    13321 aaatgactac gctccaatcc agagcggtat catgagcacc atccacgctt acaccggcga
    13381 ccagatgatc cttgacggac cacacagaaa gggcgactta agaagagcac gcgcaggcgc
    13441 agctaacatc gttcctaact ctactggcgc agctaaggca atcggcctgg ttatcccaga
    13501 actgaacggc aagctgatcg gttccgcaca gcgtgtacct gtaccaaccg gttctaccac
    13561 aatcttaaca gctgttgtta agaaggcaga tgtaactgca gaagagatca acgctgctat
    13621 gaaggctgct gcatctgagt ccttcggata caatgaagat ccgatcgtat cttctgacgt
    13681 tatcggtatg agatatggtt ctctgtttga tgcaacccag actatggttt cccatatcgc
    13741 tgatgatctg tatgaagtac aggttgtttc atggtatgac aacgagaatt cttacaccag
    13801 ccagatggta agaactatca agtacttcgc tgagttagca taatccagat cataaatcca
    13861 gattttaaag actcataagg ggtccggtct ttaggaccgg gccctttttt cgcgaaataa
    13921 atttatctat ttgcgagttt actgaaagga gatatccacc atgttaaaca agaaatcaat
    13981 cgatgatatc aatatgtccg ggaagcctac cggaaactca tagaa
//
LOCUS       DBMDUA010000062        13564 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2415_length_13564_cov_2.635891, whole genome shotgun sequence.
ACCESSION   DBMDUA010000062 DBMDUA010000000
VERSION     DBMDUA010000062.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 13564)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13564)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13564
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2415_length_13564_cov_2.635891"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..106
                     /locus_tag="ACZPCL_06515"
     CDS             <1..106
                     /locus_tag="ACZPCL_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162153.1"
                     /translation="LLEKQKEGKKRMRQIGNVEIPQKAFMSVLKLDEE"
     gene            188..1393
                     /gene="hemW"
                     /locus_tag="ACZPCL_06520"
     CDS             188..1393
                     /gene="hemW"
                     /locus_tag="ACZPCL_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR1162154.1"
                     /translation="MTNMKKKPIELYIHIPFCVKKCDYCDFLSFRALSSVHEAYVQQL
                     IREIRAQSCYCTDCQVVSVFIGGGTPSLLEPSCISRIMETVFSCFQVEPEAEITIEAN
                     PGTLLGKKLPVYRQCGINRVSIGLQSADNTELKNLGRIHSFEEFLKSFQSARMAGFTN
                     INIDLMSGIPGQTLESWKNTLKKVTMLKPEHISAYSLIIEEGTPFWNRFGEGACACDY
                     TGPALPDEDTENKIYRFTRKFLQEQGFERYEISNYAKPGKECRHNIGYWTEVAYLGLG
                     LGASSYMEGCRFTNEKDLDKYLALDFGEEDPEKRETALRKLWGQIEELSQAQRMEEFM
                     FLGLRMLKGVSDVDFVRLFGVKMETVYGDVIRRLTANGLLKKEENNLALTEWGMDVSN
                     FVLSEFLLG"
     gene            complement(1539..1844)
                     /locus_tag="ACZPCL_06525"
     CDS             complement(1539..1844)
                     /locus_tag="ACZPCL_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIR1162155.1"
                     /translation="MKYPVYLYIAVMAIVTYLIRMLPLTLIKKEIKNPFIRSFLYYVP
                     YVTLSVMTFPAIIYATASPVSGAAALAVALFFAYKGKSLFQVSIAACAIVFILEFFV"
     gene            complement(1845..2552)
                     /locus_tag="ACZPCL_06530"
     CDS             complement(1845..2552)
                     /locus_tag="ACZPCL_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR1162156.1"
                     /translation="MNEKISQFKAGLKDGIPIGLGYLAVSFTFGIMAKGAGLTTLEAV
                     VLSFTNLTSAGQFAGLGIITAGSSLVEMAMAQLIINLRYCLMSCSLSQKLDSHMPFFH
                     RFLMSYGVTDEIFGVSVCKSGVLSPYFSYGLMAMAVPGWTIGTLLGAVSGSLLPARLL
                     SALNVALYGMFLAVVIPPARENKVLRMVILVSMALSFLFTQIPVLRDISSGFKIIILT
                     FIVAGSAAVLFPVKEEE"
     gene            complement(2662..3672)
                     /locus_tag="ACZPCL_06535"
     CDS             complement(2662..3672)
                     /locus_tag="ACZPCL_06535"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR1162157.1"
                     /translation="MKKILLLGTGGTIACKRTPDGLKPVITSEEILTYVPDSRHYCEI
                     DSIQILNIDSTNIQPSHWLAVSKAIEEHYEEYDGFVITHGTDTMAYTAAALSYLIQNS
                     PKPIVITGAQKPIDMENTDARTNLADSLRFASHDKAHGINIVFDGKVIAGTRGKKERT
                     KSYNAFSSINFPYIAIIQEDHILFYLDDKAQVTEPLTFYHQMDSSVGLIKLIPSADAS
                     LLDYMAAHYDAVIIESFGVGGLPSYDDGGDYYRAVAHWINLGKTVIMTTQVTNEGSNM
                     SVYEVGKKIKREFGLLEAYDMTLEAAVTKIMWILTQTKEPAKVRELFYQTVNHDILWK
                     SS"
     gene            3849..4793
                     /locus_tag="ACZPCL_06540"
     CDS             3849..4793
                     /locus_tag="ACZPCL_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR1162158.1"
                     /translation="MMLIGGSVMFLILVVLIIFILTSCIRIVPQAQALVVERLGGYLG
                     TYGVGIHFMVPFIDRVAKKVNLKEQVEDFPPQPVITKDNVTMQIDTVVFFYITDPKSY
                     AYGVERPILAIENLTATTLRNIIGELELDETLTSRETINAKMQESLDIATDPWGIKVT
                     RVELKNIEPPAAIQEAMEKQMKAERERREAILRAEGEKKSMILVAEGNKESAVLNAEA
                     EKEAAILRAEAEKEKKIKEAEGQAEAIRAVQRATADGIRYIKEAGADQAVLQLKSLEA
                     FQAAADGKANKIIIPSDIQGIAGLVKSISEIAAKEDTK"
     gene            4877..6265
                     /locus_tag="ACZPCL_06545"
     CDS             4877..6265
                     /locus_tag="ACZPCL_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1162159.1"
                     /translation="MKEIKNKVNTNKITEGSIFGQLLLFFFPILFGTFFQQLYNTADA
                     VVVGRFVGKQALAAVGGTTSTLINLLVGFFVGLSSGATVVISQFYGARKPDKVHWAVH
                     TAVAFSLIGGVLFMIIGVVFAHMALSAMHTPEDVLTYAVVYIRIYFLGMIPNLIYNMG
                     AGILRAVGDSKRPLYFLIGSCMVNILLDLILVAGLRLGVAGAAIATISSQFFSACMVV
                     YALTHTEDMYKLVWSKVRIDGRMLQRIVRIGIPAGMQSVMYNISNIIIQSGVNTLGTD
                     NVTAWATYGKVDGLYWMMINALGISVTTFVGQNYGAGRMDRVHKGAGACMIIGTIMTA
                     IVSTLLYLKGYLLIDLFTSDAQVQEIGLSLIHFLVPTLITYITIEILSGTLRGVGDAW
                     MPLIMTGIGVCCLRVIWIIFFLPQYRSILAAAFCYPLSWTITSIAFVIYYFFFSSLRR
                     VDGRKWFQKKDY"
     gene            6295..7140
                     /gene="nudC"
                     /locus_tag="ACZPCL_06550"
     CDS             6295..7140
                     /gene="nudC"
                     /locus_tag="ACZPCL_06550"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HIR1162160.1"
                     /translation="MIQEIPYKYDPAYKDKKPELTDYLLHYEYNKVMLLKEKEGYVIP
                     TFQDLLSEEDCREKAYYLFSIGERGYYLVDDLKVPEFGSYRLEGMQIFRELEPGYQAF
                     AGITGSQIYRWRESRRFCGCCGAKMRAGTTERSMVCTDCGHTEYPKICPAVIVAIRNG
                     DKLLMSRYARGTYRRYALIAGYVEVGETFEECVRREVMEEVGLKVKNIRYYKSQPWSF
                     SDSVMIGFTADLDGDDTIRLEEDELAEAGWFSRDEIVEYPPYISVGHEMMKAFKDGTL
                     FEDQG"
     gene            7225..7881
                     /locus_tag="ACZPCL_06555"
     CDS             7225..7881
                     /locus_tag="ACZPCL_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1162161.1"
                     /translation="MKNKDYLLFDLDGTLTDPKEGITKAVQYALKHYGIQVDDLDSLC
                     PFIGPPLKDSFQKYYDFSEQQAKEAIQVYREYFSVTGWSQNKVFPGIPEMLGELKAAG
                     KKLYLATSKPEVFAKQILEHFDLASYFEFVGGADLEETRVGKGDVIQYVLDTCGLSDK
                     KEKILMIGDREHDVLGARKQGLECVGVLFGYGDRKEMEDCHAAYIAESINELRKMLLS
                     "
     gene            8012..8515
                     /gene="ilvN"
                     /locus_tag="ACZPCL_06560"
     CDS             8012..8515
                     /gene="ilvN"
                     /locus_tag="ACZPCL_06560"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR1162162.1"
                     /translation="MDDRIVLSLLVDNTAGVLSRVAGMFSRRGYNIESLTVGVTADPR
                     YSRMTVVSLGDQNVLEQIKNQLNKLEDVRDIKELHPDRSVYRELMMIKVRAGSNDRQA
                     VSAISSIFRATIVDVGKDSLTVMLTGDQSKLDALINLLEDYEILELARTGLTGLERGS
                     EDIRILP"
     gene            8657..9676
                     /gene="ilvC"
                     /locus_tag="ACZPCL_06565"
     CDS             8657..9676
                     /gene="ilvC"
                     /locus_tag="ACZPCL_06565"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR1162163.1"
                     /translation="MAKIYYQEDCNLSMLEGKTIAIIGYGSQGHAHALNLKESGCNVI
                     IGLYEGSRSWKRAEEQGFTVYTAAEAAKRADIIMILINDELQADMYKKDIEPNLKDGD
                     MLMFAHGFNIHFGCIKPPKNVDVTMIAPKAPGHTVRSEYQAGKGTPCLVAVEQDYTGK
                     ALEKALAYGLAIGGARAGLLETTFRTETETDLFGEQAVLCGGVCALMQAGFETLVEAG
                     YDPRNAYFECIHEMKLIVDLIYQSGFAGMRYSISNTAEYGDYVTGPKIVTAETKKAMK
                     KILSDIQDGTFAKEFLLDMSPAGRQVHFQAMRKLASEHPSEKVGAEIRKLYSWNNESD
                     KLINN"
     gene            complement(9927..10808)
                     /locus_tag="ACZPCL_06570"
     CDS             complement(9927..10808)
                     /locus_tag="ACZPCL_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1162164.1"
                     /translation="MEQNLSQYKIFYEVAKAGNISKAAKELYISQPAISKAISKLEDS
                     LGLSLFTRSSRGVQLTSEGEILFEHAKEAFDTLSRGEQELKRIQEFDIGHLRIGVSNT
                     LCKYVLLPYLKTFIDQYPHMKVTIESQSTAQTLSRLEQQKLDLGLVAEPSLRKDLAFI
                     PVMDIQDTFVTTPAYLNNLYVREGKDTNIFETGNIMLLDQSNMTRHHVDDYMSENHIV
                     PRQVLEVTTMDLLIEFVKIGLGVACVIKELVQKELDNGTLVELPLEIPIHRRTIGFAY
                     HPANQAMSLKTFLEFLY"
     gene            complement(10965..11849)
                     /locus_tag="ACZPCL_06575"
     CDS             complement(10965..11849)
                     /locus_tag="ACZPCL_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1162165.1"
                     /translation="MEFRELSTFLQVAKLQSFSKAAKVLGYSQAAVTIQIKQLEQELG
                     AHLFDRIGKQTTLTHQGSVFYEYAASIIRDMAQAKDAVSHPQKLTGRLCIGTIESICA
                     SIFPNLLTEYHKRHPEVNISIRTDSPDQLLEQINSNQLDLVYFMDKRVYDVKWEKVLE
                     EPEDIVFAATPDHPLAQRDEPLELDEILSYPFVLTEKDASYRLLLEQYLAAMGKHLDP
                     YLEIGNTDFILQFLRQNTGVTFLPHFTVERSVKEGYLRILPVKRFAIRTWRQLLYHKD
                     KWVTREMAEFIKLAQELS"
     gene            11977..13254
                     /locus_tag="ACZPCL_06580"
     CDS             11977..13254
                     /locus_tag="ACZPCL_06580"
                     /EC_number="4.4.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0080146 - L-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cysteine desulfidase family protein"
                     /protein_id="HIR1162166.1"
                     /translation="MDKTNEKYNAYIQILKEELIPAMGCTEPIALAYAAAVARKVLGQ
                     LPERVDIGASGSIIKNVKSVIVPNTDHLKGIPAAATAGIIAGDPDKELEVIASVTKEQ
                     VEEMKEFLKKTPISVEHVDNGLTFDIIVTLYKGDEYAKVRIANYHTNIVLIEKNGEIL
                     EQTEVAGETEEGLTDRSLLNMKDICDFINTVDVEDIKPILKRQIDYNWAIAEEGIKGN
                     YGANIGKVLLDMEGNNVRVRAKAMAAAGSDARMNGCELPVVINSGSGNQGITASVPVI
                     VYAKELKVGEEKMYRALALSNLAAIHQKTPIGRLSAYCGAVSAGAGAGAGIAYLCGGG
                     FKEIAHTVVNSLAIVSGIVCDGAKASCAAKIASAVEAGILGYNMYIRGQQFYGGDGIV
                     TKGVEETLQNVGRLGKEGMKETNEEIIKIMIGE"
     gene            complement(13356..>13564)
                     /locus_tag="ACZPCL_06585"
     CDS             complement(13356..>13564)
                     /locus_tag="ACZPCL_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR1162167.1"
                     /translation="DLECCEADGPDLEDAVENARDAARNWITAELEDTCEIPPSTHVD
                     DIELKEGMFIKNIMVIIKLLPDND"
BASE COUNT         3969 a         2694 c         3339 g         3562 t
ORIGIN      
        1 gctgttagag aagcagaagg aaggaaagaa gcgcatgcgt cagatcggca atgtggagat
       61 cccgcagaag gcctttatga gcgtgctgaa gctggatgaa gagtagaacc aatgatgctt
      121 cttgtttttg tgaataggga aacaagaaga gaacatggct gaaccagctt gaaaaagatt
      181 aatgatgata acaaacatga aaaaaaagcc cattgaactg tatatccata ttcccttttg
      241 cgtgaaaaaa tgtgattact gcgatttcct ttcatttcgt gccctgtctt cggtccatga
      301 ggcttatgta cagcagctga tccgtgagat cagggcccag agctgttatt gtacggactg
      361 ccaggtagta agcgtattta tcggcggagg aaccccctcc cttttagaac cttcctgtat
      421 cagccggatc atggaaactg tattcagctg ttttcaggta gaaccggagg cggagatcac
      481 cattgaggca aatccgggaa cgcttttagg gaaaaaactt cctgtttaca gacagtgcgg
      541 gatcaaccgc gtaagcattg gcttacagtc tgcggataat acagaactta aaaatctggg
      601 aaggattcat tcttttgagg agtttttaaa aagcttccag agcgcaagaa tggcgggctt
      661 taccaacatt aatattgacc tgatgagcgg gatcccggga cagaccctgg agtcctggaa
      721 aaatacattg aaaaaagtga ccatgttaaa accggagcat atttccgctt acagcctgat
      781 cattgaagaa ggaacgcctt tctggaaccg tttcggggaa ggcgcctgtg cctgtgatta
      841 tacgggacct gcgcttccag acgaggatac ggaaaataag atctaccgtt ttaccagaaa
      901 gttcctgcag gagcagggct ttgagcggta cgagatctca aattatgcaa aaccgggaaa
      961 agaatgccgc cataatatcg gttactggac tgaggtggca tatctgggac tgggacttgg
     1021 ggcatcctcc tatatggaag gctgtcgttt taccaatgaa aaggacctgg ataagtatct
     1081 ggcactggat tttggtgaag aagatccgga aaagcgggag acagcccttc gcaaattatg
     1141 gggtcaaata gaagaacttt cccaggcaca gcgaatggaa gagtttatgt ttttagggct
     1201 tcgtatgtta aaaggcgtgt ctgatgtgga ctttgtaaga ctgtttggtg tgaaaatgga
     1261 aacggtctat ggtgatgtga tccgcaggct gactgccaac gggctgctga aaaaagaaga
     1321 aaataatctt gcccttacag aatggggcat ggatgtaagc aattttgttt taagcgagtt
     1381 cctgctggga taaaaagcgc agaaaatctg agtataagta aaatataaaa ggtcctgaag
     1441 tgagagaaaa tctctgctcc gggacctttt ttggttattc atcttattca cttgtcaaaa
     1501 cctgccagtg acagattttc caggtcaatg caattatatt atacgaaaaa ctccaggata
     1561 aacacgatgg cacaggctgc aatggaaacc tggaacaggc tctttccttt ataagcgaaa
     1621 aacagtgcca ctgccaaagc ggcagctccg gaaacaggag aagcagttgc gtagataatg
     1681 gccgggaagg tcattacaga cagggtaaca taaggcacat aatataaaaa agagcggata
     1741 aaaggatttt tgatctcttt cttgatcagt gtcaatggca gcatacggat cagatatgtc
     1801 acaatggcca ttaccgctat gtaaagataa actggatatt tcatttattc ttcctccttt
     1861 acagggaaca gaactgcagc agacccggct acgataaatg taaggataat gatcttaaat
     1921 ccggaagaaa tatcccttaa taccggtatc tgggtgaaaa ggaagctgag tgccatggaa
     1981 acaaggataa ccatgcgcaa aaccttattt tctcttgccg gagggatgac aactgccagg
     2041 aacataccat aaagagctac atttaaagcg cttaacagcc tcgcaggaag aagactgccg
     2101 gaaacagcac caaggagggt gccgatggtc cagcctggaa cagccatagc catcaggccg
     2161 tagctgaaat aagggcttaa gaccccgctt ttacatacag atacaccgaa gatctcatct
     2221 gttacaccgt aggacataag aaaacggtga aaaaatggca tatgactgtc cagtttctgg
     2281 gaaagagaac aggacatcag gcagtaacgc aggttgatga tcagctgtgc catagccatt
     2341 tccacaaggg aagaaccggc agtaatgatc cccaggcctg caaactgacc tgcgctggtc
     2401 aggttggtaa aagagaggac tacggcttcc aaagtggtaa gtccggcccc ttttgccata
     2461 atgccaaagg taaaggaaac tgccagataa ccaagtccaa tgggaatacc atcttttaat
     2521 ccggctttga attgtgatat tttctcgttc atgtaaaacc ccccgcaata aaatacataa
     2581 aacaggcagt ctgacttaag aaaattaaga agactgcctg tttattataa caggaaatat
     2641 aaaaaagtaa agttggggcc tttaagaaga cttccacaaa atatcatggt tgacggtctg
     2701 gtagaagagc tctcttactt ttgcaggttc ctttgtctgg gtaaggatcc acatgatctt
     2761 ggtaacagct gcctccaggg tcatatcata agcttccaac agaccaaatt cacgcttgat
     2821 cttttttccg acctcataaa cagacatatt gctgccttca tttgtaacct gtgttgtcat
     2881 gatcacagtt tttccaaggt tgatccaatg agccactgca cggtagtagt cgcctccgtc
     2941 atcataagaa ggaagtccgc caacgccaaa ggactcaatg atcactgcat cataatgagc
     3001 tgccatatag tccagaaggg aagcgtctgc agatgggatc agctttatca gtcccacact
     3061 ggagtccatc tggtgataga aagtcaaagg ctctgtgacc tgcgccttgt cgtctaaata
     3121 aaacaggata tggtcttcct ggataatggc aatataagga aaattaatac tggaaaaagc
     3181 attataactc ttggtccgct cttttttacc tctggtaccg gcaatgactt ttccgtcaaa
     3241 tacaatattt atcccgtgag ccttgtcatg ggaagcaaag cgaagactgt cagccagatt
     3301 ggtacgggcg tctgtatttt ccatatcaat aggtttctgg gccccggtga tgacgatcgg
     3361 ctttggagaa ttctggatca gataggaaag agcagcagct gtatatgcca tggtatctgt
     3421 tccatgggtg atcacaaaac catcgtattc ctcataatgt tcttcaatag cttttgatac
     3481 tgccagccag tgggaaggct ggatattggt actgtctata tttaagatct gtatactgtc
     3541 gatctcgcag tagtggcggc tgtccggcac ataagttaag atctcttcgg aagtaatgac
     3601 gggtttcaga ccgtcgggtg tacgcttgca ggcaatggta ccgcctgttc ctaacagaag
     3661 gatttttttc atacaaagta tctccttgtt gagttatgat taaaagtatt aacgcttacg
     3721 attatattaa gaattcatca cttttacaat ccttgattga ataaaatcag aaatatgtta
     3781 aactatattc atacaaatta tacatggtac cgtagaatgg tatcacagta aatttcagga
     3841 ggtcaaatat gatgctgatt ggaggttccg tgatgtttct tattctggta gtactcatta
     3901 ttttcattct tacatcctgt atccgtatcg ttcctcaggc tcaggcactg gtcgtagagc
     3961 gccttggcgg ttatctggga acatatggtg taggtatcca ttttatggtg ccatttattg
     4021 acagagtggc aaagaaagta aatttaaagg agcaggtaga ggattttccg ccacagccgg
     4081 ttatcaccaa ggataatgtt accatgcaga tcgatacagt tgtatttttc tacattacag
     4141 atccgaaatc ctatgcatac ggtgtagagc gtccgatcct tgccattgaa aatctgacgg
     4201 caactaccct tagaaatatc attggtgagc tggaattaga tgagacctta acttccagag
     4261 agaccatcaa tgcaaagatg caggagtccc ttgatattgc cactgaccca tggggcatca
     4321 aggttaccag agtagaactt aagaacattg aaccgcctgc agccatccag gaagcaatgg
     4381 agaagcagat gaaggcagag cgtgagcgcc gtgaagcgat ccttcgtgca gagggtgaga
     4441 agaagtccat gatcctggtt gcagaaggaa acaaagagtc tgcagtctta aatgcagagg
     4501 cagaaaaaga ggcagccatc cttcgtgcag aagcagaaaa ggaaaagaag atcaaagagg
     4561 cagaaggcca ggcagaagcg atccgcgctg tacagagagc aactgccgat ggtatccgtt
     4621 atataaaaga agccggagca gaccaggctg tactgcagct taagagtctg gaagccttcc
     4681 aggcggcagc agacggaaaa gccaataaga tcatcatccc gtctgatatc cagggaatag
     4741 caggtcttgt aaaatctatt tctgagatcg ctgcaaaaga agatactaaa taaacatggt
     4801 gcccatcagt aagtgaaaat ttttcaactg gtgggtatcc ttttctctat ttgttggaaa
     4861 aatctgggag aaaaagatga aagagataaa aaataaggtc aataccaata agattacgga
     4921 aggcagcatt ttcggtcagc tgttattatt cttttttccg attttgtttg gcacattttt
     4981 tcagcagctt tacaatacag cagatgcagt ggtagtaggc cgttttgtag gcaaacaggc
     5041 actggcagca gttggcggta ctaccagtac cctgatcaat cttctggtgg gctttttcgt
     5101 tggcctttcc agtggtgcga ctgtagttat ttcccagttt tatggtgcca gaaagccgga
     5161 taaggtgcat tgggccgtgc atacggcggt agcgttttcg cttataggcg gtgttctgtt
     5221 tatgatcatc ggtgttgttt ttgcccatat ggctctttca gccatgcaca ctccggaaga
     5281 tgtacttaca tatgcggttg tatacatccg tatctatttc ctgggtatga taccaaacct
     5341 gatctataat atgggagccg gtatcctgcg tgcagtgggg gactccaagc gccccttata
     5401 tttccttatt ggaagctgta tggtcaatat cctcttagac ctgatcctgg tagcaggact
     5461 gcgtctgggt gttgccggtg cagccatcgc caccatcagt tcccagtttt tcagtgcctg
     5521 catggtggtt tatgcgttga cacatacaga agatatgtat aaactggtgt ggagcaaggt
     5581 gcgtattgac gggcggatgc tccagcgtat cgtgcgcatc ggtattccgg ctggtatgca
     5641 gtcggttatg tataacattt ccaatatcat tatccagtca ggtgtaaata cactggggac
     5701 agataatgta actgcctggg ctacttatgg aaaagtggac ggactttact ggatgatgat
     5761 caatgctttg ggaatttctg tcaccacttt tgtaggacaa aattatggtg ccggccgtat
     5821 ggaccgtgtg cataagggtg ccggggcctg tatgatcatt ggaacgatta tgacagcgat
     5881 cgtatccacc ttattatact taaaaggata tctgctgatt gatctgttta catcagatgc
     5941 acaggtacag gagatcggtt taagtctgat ccatttcctg gtgcctacat tgatcaccta
     6001 tattaccata gagatattat ccggtacact gcgcggtgta ggagatgcct ggatgccgtt
     6061 gatcatgacg gggattggcg tttgctgtct gcgtgtgatc tggatcatct ttttcttgcc
     6121 acagtacaga tccatcctgg cagctgcgtt ctgctatcct ttatcatgga ccattacttc
     6181 cattgccttt gttatttatt atttcttctt cagcagtctg cggcgcgtgg acggaagaaa
     6241 gtggtttcag aaaaaggatt attaaaagag ctattaaagg agtcgtaagt taaaatgata
     6301 caggagatac cttataaata tgatccggca tacaaagata aaaagccgga actcacagac
     6361 tatcttttgc attatgaata caataaagta atgcttttga aagaaaaaga aggttatgtg
     6421 atccctacct ttcaggatct tctttcagaa gaagactgcc gggaaaaggc gtactatctg
     6481 ttttccatcg gtgaaagagg ctattatctg gtagatgatc tgaaagtgcc ggaatttggc
     6541 agctaccgcc tggaaggaat gcagatcttc agggaactgg aaccggggta tcaggcgttt
     6601 gcaggtatta caggaagcca gatctaccgt tggagagaaa gcaggaggtt ctgcggctgc
     6661 tgtggcgcaa agatgagagc tgggactaca gaacggtcca tggtgtgtac agattgcgga
     6721 catacagagt atccaaagat ctgtccggct gtgatcgtag ctatccgaaa tggggacaaa
     6781 ctgcttatgt cccggtatgc aagaggcact taccgcaggt atgcactgat cgcaggttat
     6841 gtggaagtgg gcgagacatt tgaagaatgt gtacgcaggg aagtaatgga agaagtaggc
     6901 ctgaaggtga aaaatatccg ctattacaag agccagccat ggtccttttc tgactctgtg
     6961 atgatcggtt ttaccgcaga cctggatgga gatgatacca tccggttaga ggaagatgaa
     7021 ctggcagagg ctggttggtt tagccgggat gagatcgtag agtacccgcc ttatatcagc
     7081 gtgggacatg agatgatgaa ggcgtttaaa gacgggactc tttttgaaga ccagggatga
     7141 tccgaaattg atataaaata aggtaatgcg atcacagact ggtacgaaag ctgaatattt
     7201 gtttagaagg gaattaatta caacatgaaa aataaagatt atctgctgtt tgacctggac
     7261 ggaacgctga cagatccaaa agaggggatc acaaaggcag ttcagtatgc attaaaacac
     7321 tatgggatcc aggtggatga tctggacagt ttatgtcctt ttatcggtcc gccattaaaa
     7381 gacagcttcc agaaatatta tgatttttct gaacagcagg caaaggaagc gatccaggtg
     7441 taccgtgaat atttttccgt tacaggctgg agccagaata aagtatttcc aggcattccg
     7501 gaaatgttag gagaattaaa agcagcggga aagaagctgt atcttgcaac ctccaaaccg
     7561 gaggtatttg caaagcagat cctggaacat tttgatctgg cttcttattt tgagtttgtt
     7621 ggaggcgctg acctggagga gacccgtgta ggaaaagggg atgtgatcca gtatgtttta
     7681 gatacctgcg gcctttctga caaaaaagaa aagatcctga tgatcggtga cagagaacat
     7741 gatgtattgg gggccagaaa gcagggatta gagtgcgtag gcgtcctgtt tggctatggt
     7801 gacaggaagg aaatggaaga ctgccacgct gcttatatag ctgaaagtat taatgaattg
     7861 agaaaaatgc tcttatcata accagaaagc ataatatcca ttactattag gcaattgaca
     7921 aagtgggtaa tggttgttat tataaaaact aatatgtggg acgctgaaag acatttgtct
     7981 gcggcggacc gatatggagg caaatgatat gatggatgac agaatcgtgt tatcattgtt
     8041 agtggataat acggcaggtg ttctgtcccg tgtagcgggc atgttcagcc gccgtggata
     8101 taatattgag agcctgacag taggtgttac tgcagaccca aggtattcca gaatgaccgt
     8161 tgtgtcactg ggagaccaga atgttcttga gcagatcaag aaccagttaa ataaactgga
     8221 agatgtaaga gatatcaagg aacttcatcc ggatcgctct gtatacaggg agcttatgat
     8281 gatcaaggta agagccggat caaatgaccg tcaggcggtc agcgccatct caagtatttt
     8341 ccgtgcaacc attgtggatg tgggaaaaga ttccctgact gttatgctga caggagacca
     8401 gtcaaaactg gatgcgctca ttaatctgct agaagattat gagatcctgg aactggcccg
     8461 taccggactc accggactgg agcgtggcag cgaagacatc cgtattttgc cataaggcaa
     8521 aatgaaaaaa gagcttaagg gggcagccaa aggcgaacag agaggcgtcg gacttgctgt
     8581 cttaacataa atatgccagt gaatttgctg gtgataagtt ttatactttt atacgatagt
     8641 aggaggaaaa aacgctatgg caaagattta ttatcaggaa gactgtaacc tttcaatgtt
     8701 agagggaaag accatcgcta ttattggtta cggcagccag ggacatgcac atgcattaaa
     8761 cttaaaggaa tccggatgca atgttatcat tggtctgtat gagggaagcc gctcctggaa
     8821 gagagcagaa gagcagggct tcactgtata tactgcagca gaggctgcaa agagagcaga
     8881 tatcatcatg atcctgatca acgatgagct gcaggcagat atgtacaaga aggacatcga
     8941 gccaaactta aaggatggcg atatgctgat gttcgcacat ggcttcaata tccacttcgg
     9001 atgcatcaag ccaccaaaga atgtagatgt taccatgatc gctcctaagg caccaggcca
     9061 cactgtacgt agtgagtacc aggctggcaa gggaactcct tgtctggtag ctgtagagca
     9121 ggattataca ggaaaagcac tggagaaagc actggcttac ggccttgcaa tcggtggtgc
     9181 aagagcaggt ctgttagaga ccaccttccg tactgagaca gaaactgacc tgtttggtga
     9241 gcaggctgtt ctttgcggcg gtgtttgcgc tttaatgcag gctggtttcg agactctggt
     9301 agaagctgga tatgatccaa gaaacgctta cttcgagtgt atccatgaga tgaagctgat
     9361 cgtagacctg atctatcagt ctggtttcgc tggaatgcgt tactctatct ccaataccgc
     9421 tgagtatggt gattatgtaa ccggacctaa gatcgttacc gctgagacta agaaggctat
     9481 gaagaagatc ctgtccgata tccaggatgg taccttcgct aaggaattct tacttgatat
     9541 gtccccagca ggccgtcagg tacacttcca ggctatgaga aagcttgctt ccgagcatcc
     9601 atcagagaag gttggtgctg agatccgtaa gctgtacagc tggaataatg agtctgataa
     9661 gctgatcaac aactaagcac aattttactt ttgtgaaatt gcgcgctact caaacgtaat
     9721 gtaatggagt ttgagtttcc cgtacacgct acccaaacgt agcgaagcgc gtataaaata
     9781 ttaaaactcc cggaagttca gaaggctgtg acctttgaaa ttccgggagt ttttttgcgg
     9841 ttttttatat agcggcatgg cgtaaaacca ttttttcaag agagcccctc tcaccacccg
     9901 cgcgtcagcg cgggtccaaa tctgttttaa tataaaaatt ccaaaaacgt cttcagtgac
     9961 attgcctggt ttgcgggatg ataggcaaaa ccaatggtcc gtctgtggat cggtatttcc
    10021 agtggtaatt ctaccagtgt accgttatcc agttctttct gtaccagttc cttgataaca
    10081 caggcaaccc caagtccgat ctttacgaat tcaatcagca ggtccattgt ggttacttcc
    10141 aatacctgtc ttggtacaat atgattttca gacatgtaat catctacatg atgccgggtc
    10201 atattgctct gatctaaaag catgatattt cctgtctcaa aaatattggt atctttacct
    10261 tcccgtacat acagattgtt cagataagcc ggtgtagtga caaaggtatc ttggatatcc
    10321 atgaccggga taaacgccag atccttgcga agggatggct ctgccactaa gccaaggtcc
    10381 agtttctgct gttccagcct ggaaagcgtc tgggctgtgg actggctttc gatagtcact
    10441 ttcatatggg gatactggtc aataaaggtt ttaagataag ggagcagtac atatttacag
    10501 agagtattgc tgacaccaat gcgcagatgt ccgatgtcaa attcctggat ccgttttaac
    10561 tcctgttcac ctctgcttaa agtatcaaaa gcctcttttg catgttcaaa caaaatctcc
    10621 ccttcagaag tcagctgaac tccccgggag ctgcgggtaa acagagaaag ccccagactg
    10681 tcctctaatt tgctgatggc cttgctgatg gcaggctggc tgatataaag ttctttggca
    10741 gctttggaaa tgttgcctgc ttttgctact tcataaaaga ttttatattg agaaagattt
    10801 tgttccatga agacctccaa aattctgtaa gtatgtttaa aatacatgta taacattaat
    10861 tcatacatac catatatttt tattatatca gcacatcata gaaaatgtaa agtacagaat
    10921 aaaggacctt cttatctaaa gtttatggaa cagaaaataa gggattaaga aagttcctgg
    10981 gccagcttga taaactccgc catttcccgt gttacccatt tatctttgtg atagaggagc
    11041 tgtctccagg tacggatggc aaaacgcttt accggaagga tgcgcagata accttctttt
    11101 acggatcgtt ctactgtaaa atggggaagg aaggtaacac ctgtattctg tcttaaaaac
    11161 tgcaggataa agtcagtgtt tccaatctcc agataaggat ccagatgctt gcccatagca
    11221 gccagatact gctctaagag caggcggtag ctggcatctt tttcagtaag aacaaaagga
    11281 taggaaagga tctcatccag ttccagtggt tcgtcacgct gggccaaagg atggtccggt
    11341 gtagctgcaa atacgatatc ctcaggttct tctaatactt tttcccattt cacatcatag
    11401 acccgcttgt ccataaaata aaccagatcc aactggttgc tgttgatctg ctctaacagc
    11461 tggtccgggg agtcggtgcg gatgctgatg ttcacttccg ggtgacgttt gtggtactcc
    11521 gtcaggaggt tggggaaaat agatgcgcag atagactcaa tggtcccgat acacaggcgg
    11581 cccgtcagtt tttggggatg ggaaacagca tcttttgcct gcgccatgtc ccgtatgatc
    11641 gaggctgcgt attcataaaa aacactgccc tggtgtgtca gggtggtctg tttgccgatc
    11701 cggtcaaaga gatgagcccc cagttcctgc tctaactgtt tgatctgtat ggtaacagcg
    11761 gcctgggaat atccaaggac tttggcggct ttggaaaaac tttgcagctt tgccacctga
    11821 agaaaggttg acagttcacg aaattccata gaatgacctc gcttatctat aaaagttttt
    11881 aaacaatatg tttaaaacgt taaattttac attaggatag ttctatggta cagtaagtgc
    11941 agtaataaat caagggcaaa tctggaggct gtgaaaatgg acaagacaaa tgaaaaatac
    12001 aatgcgtata ttcaaatttt aaaagaggaa ctgatccctg ctatgggatg tacagagccg
    12061 atcgctctgg cttatgcagc agcagttgca agaaaagtac tgggtcagct gcctgagaga
    12121 gtagacatcg gcgcaagcgg aagcattatc aagaatgtaa agtcagtaat cgtaccaaat
    12181 accgatcact taaagggaat tccggcagct gcaactgcag gtatcattgc aggtgatcct
    12241 gataaggaac tggaagttat cgcaagtgta accaaagagc aggtagagga gatgaaggaa
    12301 tttctgaaaa agactcctat ttctgtagaa catgtagata acggactaac ttttgacatt
    12361 atcgtaaccc tgtacaaagg ggatgaatac gcaaaggtac gtattgccaa ctatcacacc
    12421 aatattgtcc tgatcgaaaa gaacggtgaa attttagagc agacagaagt agctggtgaa
    12481 acagaagaag gcctgacaga cagaagcctt ttaaatatga aagatatctg tgattttatt
    12541 aatacagtag atgtagaaga tatcaaaccg atcttaaaac gtcagatcga ttataactgg
    12601 gcgatcgcag aagagggaat taaaggaaac tacggagcaa acattggtaa ggttctgcta
    12661 gatatggaag gaaacaatgt gcgtgtccgt gcaaaagcaa tggcagcagc tggttctgac
    12721 gcccgtatga acggatgtga actgccggta gttatcaact ccggaagcgg taaccaggga
    12781 attacagctt ctgtacctgt tatcgtatat gcaaaagaat taaaggtagg agaagagaag
    12841 atgtatcgtg ctcttgcatt atccaacctt gcagcgatcc atcagaaaac accgatcggc
    12901 cgtctttccg catactgtgg tgcagtcagt gcaggtgcag gcgcaggtgc aggtatcgca
    12961 tatctgtgcg gcggcggttt caaagagatc gcacatacag tagtaaattc actggctatc
    13021 gtatccggta ttgtctgtga cggtgcaaag gcatcctgtg cagcgaagat cgcatctgca
    13081 gtagaagcag gtatcttagg atacaatatg tacatcagag gccagcagtt ctacggcggt
    13141 gacggaatcg ttaccaaggg tgtagaagag accttacaga atgtaggccg tcttggaaaa
    13201 gaaggaatga aagaaacaaa cgaagagatc atcaagatca tgatcggaga ataaaaaatg
    13261 acgttcccgc tgcctgcggt aagcagaata gatctgtttt agaatgaaaa atccctgagc
    13321 acatgttata gtgtgttcag ggatttttta acacatcagt cgttatcagg cagcagcttg
    13381 ataatgacca tgatattctt aataaacata ccttccttta actcaatatc atctacatgg
    13441 gtagaaggcg ggatctcaca ggtatcttcc agttctgcag tgatccagtt tctggctgcg
    13501 tctctggcat tttcaacagc atcctctaag tccggtccgt ctgcctcaca acattccaga
    13561 tccg
//
LOCUS       DBMDUA010000063        13527 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2418_length_13527_cov_3.096455, whole genome shotgun sequence.
ACCESSION   DBMDUA010000063 DBMDUA010000000
VERSION     DBMDUA010000063.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 13527)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13527)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13527
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2418_length_13527_cov_3.096455"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            68..1288
                     /locus_tag="ACZPCL_06590"
     CDS             68..1288
                     /locus_tag="ACZPCL_06590"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR1162168.1"
                     /translation="MLNKKSIDDINVKGRRVLVRCDFNVPLKNGEITDETRIVAALPT
                     IQKLINDGAKVILCSHLGKVKNGPNEGESLAPVAKRLSEKLGKEVVFVSDYNVTGEAA
                     TKAVEAMKEGDVVLLQNTRFRGAEETKNGEEFSKELADLADDYVCDAFGSSHRAHASV
                     AGVTKFISAKGGSNVVGYLMQKEIDFLGNAVENPVRPFVAILGGAKVADKLNVISNLL
                     EKCDTLIIGGGMAFTFLKAEGKEIGKSLVDDTKLDYCKEMMAKAEKLGKKLLLPVDAV
                     VAASFPDPIDAEIPVENVSVDAIPDDKMGLDIGAESAKLYAEAVKSAKTVVWNGPMGV
                     FENPVLAKGTIAVAQALADTDATTIIGGGDSAAAVNQLGFADKMSHISTGGGASLEFL
                     EGKELPGVAAADDK"
     gene            1384..2136
                     /gene="tpiA"
                     /locus_tag="ACZPCL_06595"
     CDS             1384..2136
                     /gene="tpiA"
                     /locus_tag="ACZPCL_06595"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR1162169.1"
                     /translation="MARKKIIAGNWKMNMTPSEAVALVNELKPLVVNDEVDVVFCVPA
                     IDIIPAMEAAKGTNIQIGAENMYFEEKGAYTGEISPNMLNDAGVKYVIIGHSERREYF
                     AETNETVNKKVLKAFEHGITPIICCGESLTQREQGITIDWIRQQIKIAFLNVTADQAK
                     TAVIAYEPIWAIGTGKVATTEQAEEVCAAIRACIGEIYDEATAQAIRIQYGGSVSAAS
                     APELFAQPDIDGGLVGGASLKPDFGKIVNYNR"
     gene            2383..3927
                     /gene="gpmI"
                     /locus_tag="ACZPCL_06600"
     CDS             2383..3927
                     /gene="gpmI"
                     /locus_tag="ACZPCL_06600"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR1162170.1"
                     /translation="MSKKPVVLIILDGYGLNDNCEHNAVCEGKTPVMDQLMSQCPFVK
                     GQASGLAVGLPEGQMGNSEVGHLNMGAGRVVYQELTRITKSINDGDFFNVPEFLTAVE
                     NCKKNNSALHLFGLVSTGGVHSHLNHIYGLLELAKRNGLEKVYVHCFLDGRDTPPESG
                     KEFVTELEAKMAELGTGKVASVMGRYYAMDRDKRWDRVELAYKALTKGEGNTAVSATE
                     GIQASYDAGKSDEFVVPFVVTENGKALATINDGDSVIFFNFRPDRAREITHAFCDDNF
                     DGFAREKKLDLVYVCFTDYDETITNKLVAFKKESITNTFGEFLAANGKKQVRIAETEK
                     YAHVTFFFNGGVEEPNEGEDRILVPSPKEVATYDLKPEMSAYGVCDKLTEAIKSGKYD
                     VIIINFANPDMVGHTGVEAAAIKAIEVVDECVGRAVEALKEVNGVMFICADHGNAEQL
                     VDYETGEPYTAHTTNPVPFILVNADADMKLREGGALCDIAPTLIELMGMEQPKEMTGK
                     SLIVRK"
     gene            complement(4123..5541)
                     /locus_tag="ACZPCL_06605"
     CDS             complement(4123..5541)
                     /locus_tag="ACZPCL_06605"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HIR1162171.1"
                     /translation="MEKNMDMMKFAPEPFKIKMVENMARLDKEERKEAIKTAGYNTFL
                     LKSEECFIDLLTDSGTNAMSDHQWAGLMLGDEAYGGSRNFYHLEETVRELFGFKYVVP
                     THQGRGAENILSSLTIKPGDYVPGNMYFTTTRFHQERNGATFRDVIIDEAHDPSAILD
                     FKGNIDLNKFQALIDEVGAEHIPYICLAVTVNLAGGQPVSMANIKAVSELAHKYGIKV
                     MYDATRCVENAYFIKTREPGYENKTIKEIVHEMFSYGDGCTMSGKKDCLTNIGGFLCM
                     NDHDLYVRATGMVVQFEGMPSYGGMAGRDMEAMAIGLRESMNFDYISHRVNQIRYLGE
                     KLDAAGVPMVKPYGGHAIFVDARAFLDHLDQEEDFPAQALSAAIYEFSGVRTMERGII
                     SAGRDPHTRENHVPKLETVRLTIPRRVYTYAHMDYVADAIIGLYQRRHDISGLKWVYE
                     PEVLRFFTGRFEPKNGELIKGF"
     gene            complement(5624..7111)
                     /locus_tag="ACZPCL_06610"
     CDS             complement(5624..7111)
                     /locus_tag="ACZPCL_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1162172.1"
                     /translation="MIIKLVGAINDLLWGDLIILEFSGGEVQFGLSLLVLILIPAGLY
                     FTFKTKFLPFRLFPEMIRVTLEKDEAKKDENSISGVQALIVATATRVGMGNLAGVVAA
                     ISFGGAGAVFWMWLTALIGSSSSFVESTLAQIYKEKDPLYGGYRGGPAYVMDRIRLVT
                     KIKREDVFVKNVKEEAEYVADDEQTYYTRGCKYGLLGTAFALSGLLCWAGISQVIGNS
                     VTTSFQNAFGIPQVATATILVAMSAVIVLRKNATVKVLDMVVPVMAGAYFLVTLFVII
                     KNITLLPSVIGNIFSQAFGIRQFAGGGLGVIVMNGVKRGLFSNEAGSGSAPCAAAAAE
                     VSHPVKQGLIQALGVFIDTLVICSCSAFLMLLAPAEKIEGLMGMDLLQAAMNHHLGYA
                     GVVFIAVVLFLFSFSTFLGILYYARCNVSYVFGDTWKAQTGYKIFLLIMLFIGGVAAY
                     EFVWELGDLGVGLMTVFNMIAIVPLSGQAINALKDYETNYKKKKA"
     gene            7614..8288
                     /locus_tag="ACZPCL_06615"
     CDS             7614..8288
                     /locus_tag="ACZPCL_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1162173.1"
                     /translation="MPEKIIKQTLSEQIYNILKEDILSGRILMGDKLTNRELQERFQV
                     SSTPVRDAINRLSKDGLTQEVSKSGAQLITFDYSYANELNDFITFLACRAVMLSSTEP
                     DTEKLVEKLQMYEEEMAGAKTSMEYFEADFRYHKVFFDHCGNRFMKETYKQYNFIRTL
                     LIRYAIRTEDERDLGIRQHRGITQAFQKKDYEKARKLMEEHYLSGMQLAREHYREGDT
                     FRENKK"
     gene            8322..9191
                     /locus_tag="ACZPCL_06620"
     CDS             8322..9191
                     /locus_tag="ACZPCL_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1162174.1"
                     /translation="MRTAIATDSNSGITQEQAAKLGIYVLPMPFMINGEMFYEGVNLT
                     HEEFFKRMEEGADITTSQPSPGEVTDFWDKLLKEYDEILYIPMSSGLSGSCQTAIMLA
                     DDYDGKVQVVNNQRISVTQEQSALDARDLAAKGYEAAQIKEILERTKYESSIYITLTT
                     LKYLKKGGRITPAAAALGTLLRIKPVLTIQGEKLDAFSKARTAKQAKTIMLTALAKDL
                     EERFHDKNAEHTYLEIAHTCNEEGAKELEEALKEQYPGVPITIAPLSLSIACHIGPGS
                     FAVACSSKLKELE"
     gene            9193..10662
                     /locus_tag="ACZPCL_06625"
     CDS             9193..10662
                     /locus_tag="ACZPCL_06625"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1162175.1"
                     /translation="MEKEKWYEKLKAQGQSITVPMTLLMIAVAVIPLLLQTWILIGSF
                     KQNQIEARSIEIQNQCVILSNRMTRSGYMTADKANNVSLDAQMQTIADLYNGRIVIVN
                     SSYKIMTDTFNLSTGKYYISEEVIRCFKGESGSSFNKEGGYFYQTIPVYDPADEKKIQ
                     GVIAVTASMENVVSVTERVKSSAQLFFLCMALVAILFSIMSAHFLVRPFKRLLRSFDR
                     VAQGDLDADISEDAYSETRQMSQAVQKSLRKLKAVDQSRQEFVSNVSHELKTPITSIR
                     VLADSLMGMEDAPVELYREFMADISDEIDRENQIIEDLLTLVRMDKSAESQMNISQVH
                     INHELEMILKRLRPIAKRSNVELILESIREVTADVDKVKLNLAVTNLVENAIKYNIDS
                     GWVRVTLDADHKYFYVKVADSGIGIPEDSVDHIFERFFRVDKARSREVGGTGLGLSIT
                     KNVIQMHHGVIDVESTPGEGTTFSMRIPLNYVPRQEERL"
     gene            10659..11606
                     /locus_tag="ACZPCL_06630"
     CDS             10659..11606
                     /locus_tag="ACZPCL_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HIR1162176.1"
                     /translation="MKHGKRKFLLSILLGVLVAAMAVLSGCKARTEPKSDPGAGKYLI
                     YYLNSSVTKLVAQEYETETKDQVALAEELVDQLTYVPRDLDCQTVLNDKVSFKACRID
                     EQVLYLYFDGSYTAMKPEQEILCRAALARTMTQIPGIDYISIYCGDQPLMDRQGNPVG
                     FISAGDFIINTSNVNAYEKTELTLYFANSTGDKLVAEKREVAHNINTSMEQLIVEQLI
                     AGPSDSSHQAVLPSDCKILSVSVTDNICYINFDSAFLNSNQAVNEYIPIYAIVNSLVD
                     KTSVSKVQIMVNGSQDVMFREVVSLNTAFERNTDYIEQE"
     gene            11668..>13527
                     /locus_tag="ACZPCL_06635"
     CDS             11668..>13527
                     /locus_tag="ACZPCL_06635"
                     /inference="COORDINATES: protein motif:HMM:NF015714.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR1162177.1"
                     /translation="MKRPLVVITAGFVLGEVLALCLQEAVYRIFFWFAGVLFLTVLAV
                     RRFLSRSVMYRTLLLIFVFVFGGMTAGSFSGARCREQMEQEEKRIIQAGFGDVVQLTG
                     QVVDVREKSEKLEIIVNQVKVGKRVRNIADPDDIRWLSLQYPVQLSGTPGDLEGWEEK
                     LLPGKQVKMACSFRLTEPAKNPGEFDAKLYYRSKGVMCTGYIKALKESSGADRPFMRL
                     ILWFRRYCSHVLEEYGGKEDSSIYKAMLLGDTSFMETDTLDLYRSSGIAHLLAVSGQH
                     LSLIGGGMYLLLRKTVGGFRASGIIGGGFVISYGIFTGSSGSAIRAVLMICCLWLAAE
                     RGRTYDSLSALSFAALGLLGNNPYLIFHSGFQLSFAAVLAISGPGQWMIREYNIGKNW
                     EKTVVISLWVQIFLLPVMAWHYYQYPLYGMFLNFLVLPLVPVLMIAGIMILLTGGFLP
                     LAAGTAAKVGHVILGYYKWICEGSLKLPGAVRIMGRPGPGQILGYIVLWGIAVALLIW
                     QRRGQTRKILKKYINLIFAVATLLTAVLFSFAILSLHPVRGFELLCLDVGQGDGFLLR
                     SGTTNILIDSGSSDQKKLGSRTLEPCLKSKGISRLDIAVVSHGDSDHISGLLYL"
BASE COUNT         3913 a         2754 c         3437 g         3423 t
ORIGIN      
        1 ggaccgggcc cttttttcgc gaaataaatt tatctatttg cgagtttact gaaaggagat
       61 atccaccatg ttaaacaaga aatcaatcga tgatatcaat gtaaagggaa gaagagtttt
      121 agtccgctgc gacttcaacg taccgttaaa gaacggcgag atcacagatg agacccgtat
      181 cgtagcagct ctgccaacca tccagaagct gattaacgac ggcgcaaagg ttatcctgtg
      241 ctcccactta ggaaaagtta aaaatggccc gaacgaaggc gagtctttag ctccagtagc
      301 aaagagactg tctgagaaat taggcaagga agttgttttc gtatctgatt acaatgtaac
      361 cggcgaagca gctaccaagg ctgtagaagc tatgaaggaa ggcgatgtag ttcttctgca
      421 gaatacacgt ttccgcggcg ctgaagagac caagaacggc gaagagttca gcaaggaact
      481 ggctgatctg gctgatgatt atgtatgcga cgcattcggt tcctcccaca gagcacatgc
      541 ttctgtagca ggcgttacca agttcatctc tgcaaagggc ggctccaacg tagttggtta
      601 cttaatgcag aaagagattg atttcttagg aaatgcagtt gaaaatccag taagaccttt
      661 cgttgctatc ttaggcggtg ctaaggttgc tgataagtta aatgttatct ctaacctcct
      721 tgaaaagtgc gatacactga tcatcggcgg tggtatggca ttcaccttct taaaggctga
      781 aggcaaagag atcggtaagt ccttagtaga cgataccaag ttagattact gcaaggaaat
      841 gatggcaaag gctgagaagc ttggcaagaa gcttttactt ccagtagatg ctgtagtagc
      901 agcttccttc ccagatccga tcgatgctga gatccctgta gagaacgttt ctgtagacgc
      961 tatcccagat gacaagatgg gtctggatat cggtgcagaa agcgcaaagc tgtatgctga
     1021 agctgttaag agcgctaaga ccgttgtttg gaacggaccg atgggcgtat tcgagaaccc
     1081 tgttcttgca aagggaacca tcgcagtagc tcaggcttta gctgatactg acgcaactac
     1141 cattatcggt ggtggtgact ctgcagcagc agtaaatcag ttaggatttg ctgataagat
     1201 gagccacatc tctaccggcg gcggcgcttc tctggaattc ctggaaggaa aggaactgcc
     1261 aggcgtagct gcagcagatg acaaataaag ataactgata ttgcaggcgg ctgtttttga
     1321 aataactggg gataaaaaca gaccgcctgt gagtcgtgta tgtgtataaa ggagacggaa
     1381 gttatggcaa gaaagaagat catcgcaggc aactggaaga tgaacatgac accttctgag
     1441 gcagttgctc ttgtaaacga attaaaacca ttagtagtaa atgatgaagt agatgtagta
     1501 ttctgtgtac cggctattga tatcattccg gcaatggaag cagcaaaggg aaccaacatc
     1561 cagatcggtg ctgagaacat gtattttgag gaaaagggcg cttatacagg cgagatctcc
     1621 ccaaacatgt taaatgacgc aggcgttaag tatgtgatca tcggccactc tgagagaaga
     1681 gagtactttg ctgaaacaaa tgagaccgta aacaagaaag tattaaaggc atttgagcat
     1741 ggaattactc ctatcatctg ctgcggtgaa tctttaactc agagagagca gggcattacc
     1801 attgactgga tccgtcagca gatcaagatc gctttcttaa acgtaacagc agatcaggca
     1861 aagacagctg ttatcgctta cgagccgatc tgggctatcg gaaccggtaa ggtagcaacc
     1921 acagagcagg ctgaagaagt atgcgcagcg atccgcgcat gcatcggcga gatctatgat
     1981 gaggcaactg cacaggctat ccgtatccag tacggcggtt ctgtatcagc agcaagcgca
     2041 ccagaactgt ttgcacagcc ggatatcgac ggcggcctgg taggcggcgc ttccttaaag
     2101 ccagactttg gtaagatcgt aaactataac agataattac tcaggacggc gggaaatttg
     2161 acccaaaaag gagcgtcaag cttgcttgta agcgcctttt tggggcagat ttcacattgg
     2221 agtgacctcc gatgcttctt gtgcggcaaa gccgtgcaag aagatgcgcc gtccgggtgg
     2281 ttgcgaattt agccctgggg tcgtgaaaac gggtctcagg gcatttgtat ccattttata
     2341 aagccttagt aaaaaaggca gatttaagag gagaaaacag atatgagcaa gaaaccagta
     2401 gtattaataa tcctggacgg ctatggactg aatgataact gtgagcacaa cgcagtatgc
     2461 gaaggaaaaa ctcctgttat ggatcagtta atgagccagt gtccatttgt aaaaggccag
     2521 gcaagcggcc ttgcagtagg acttccggaa ggacagatgg gtaactccga ggtaggacac
     2581 ttaaatatgg gcgcaggccg tgttgtatac caggagctga ccagaattac caagtcgatc
     2641 aacgacggtg atttctttaa tgtaccagaa tttttaacag cagtagaaaa ctgcaagaaa
     2701 aacaattccg ctctgcacct gttcggtctg gtatctaccg gcggtgtaca cagccactta
     2761 aaccatattt acggcctgtt agagctggca aagagaaatg gcctggaaaa agtatatgta
     2821 cactgcttct tagacggtcg tgacacacca ccggaatccg gcaaggaatt tgttactgag
     2881 ctggaagcaa agatggcaga gcttggcaca ggcaaggtag catctgttat gggacgttac
     2941 tatgcaatgg accgtgataa gagatgggac cgtgtagagt tagcttacaa ggctcttaca
     3001 aagggcgaag gaaatacagc agtaagtgca acagaaggca tccaggcttc ctatgatgca
     3061 ggaaagtccg atgagttcgt tgtaccattt gtagttactg aaaacggaaa agcactggca
     3121 accatcaatg acggtgactc cgttatcttc tttaacttcc gtccggaccg tgcaagagag
     3181 atcacccatg cattctgcga tgacaatttt gacggttttg caagagagaa gaagttagac
     3241 ctggtatatg tatgcttcac cgattatgat gaaaccatta ccaacaagct ggttgcattt
     3301 aagaaagagt ccattaccaa tacctttggc gagttcctgg cagcaaacgg caagaagcag
     3361 gtaagaattg ctgagacgga aaaatatgct cacgttacct tcttctttaa cggcggcgta
     3421 gaagagccaa acgaagggga agaccgcatc ctggttcctt ctccaaagga agttgctacc
     3481 tacgacttaa agccggagat gagcgcttac ggcgtatgcg ataagttaac tgaggcgatc
     3541 aagtccggca aatatgatgt gatcatcatc aacttcgcta acccggatat ggttggacat
     3601 acaggcgtag aagcagcagc aatcaaggca atcgaagtag tagatgagtg tgtaggccgt
     3661 gctgtagaag cattaaagga agtaaacggc gttatgttta tctgtgctga ccacggaaac
     3721 gcagagcagt tagtagacta tgagacggga gagccataca ccgctcatac taccaacccg
     3781 gttccattta tcctggtaaa tgcagacgct gacatgaagc ttcgtgaagg cggcgcactg
     3841 tgcgatattg caccgaccct gatcgaatta atgggtatgg agcagccaaa agagatgact
     3901 ggtaagtcac tgatcgttag aaagtaataa gtaaaaataa gcagacgcgc ctttagggcg
     3961 cgtttgttgt atatataatg tatgggtcca tgaaacggcc tactaaatgt gacgggaatt
     4021 ataaatgcat tgcatcaaaa atccggaggc agcccatgtg ggccgccccc ggatttttta
     4081 ggagtaactg ctataaataa gaggtgattg cgtgctttct tattagaagc ccttaatgag
     4141 ttcgccgttc tttggctcga aacgtccagt gaagaagcgg agaacttctg gctcgtaaac
     4201 ccatttcaga ccgctgatgt catgtcttct ctggtacagg ccgatgatag cgtcagcaac
     4261 ataatccata tgagcgtatg tataaacacg acgcgggatt gtaagacgaa cggtttccag
     4321 tttcggaacg tggttttcac gggtgtgtgg atcacgtcct gcagagatga tgccgcgctc
     4381 cattgtacga acgccggaga actcgtagat tgctgcggag agtgcctgag ctgggaagtc
     4441 ctcttcctga tctaagtgat ctaagaatgc tcttgcatct acgaagatcg cgtgtccgcc
     4501 gtaaggttta accattggaa cacctgctgc gtctaacttt tcgcccaggt aacggatctg
     4561 gtttacacgg tggctgatgt aatcgaagtt catggactcg cgaagaccaa tagccattgc
     4621 ttccatatct cttcctgcca taccgccgta agaaggcatt ccttcaaact gtacaaccat
     4681 tccagttgcg cgaacataca ggtcatgatc gttcatacac aggaaaccac cgatgttggt
     4741 taagcagtct ttcttaccag acattgtaca gccatcgccg taggagaaca tttcatgaac
     4801 gatctctttg atggtcttgt tctcgtagcc aggttcacgg gtcttgataa agtatgcgtt
     4861 ctcaacgcaa cgggtagcat cgtacataac cttgatgccg tatttgtgag ccagttcgga
     4921 aacagctttg atattagcca tggaaacagg ctgtccacct gccaggttta cggtaacagc
     4981 gaggcagata taaggaatgt gctctgcgcc aacttcgtcg atcagtgcct ggaatttatt
     5041 taagtcgatg ttgcccttga aatcaaggat tgcacttgga tcatgagctt cgtcgatgat
     5101 aacatcacgg aaagtagcac cattgcgctc ctgatggaaa cgggtggtag taaagtacat
     5161 gttgcctgga acataatcgc ctggcttgat ggtcagagag gagagaatgt tctctgcgcc
     5221 acgtccctga tgagtaggaa ctacatattt aaagccaaac agttcacgaa cagtttcttc
     5281 caggtgatag aagttgcggg atccaccgta agcttcgtcg cctaacataa ggccagccca
     5341 ctgatgatca ctcattgcat tggttccgga gtcagttaaa aggtcgataa agcattcttc
     5401 agactttaat aagaatgtat tgtaaccagc agtcttgatc gcttctttac gctcttcttt
     5461 gtctaagcgt gccatgttct ctaccatttt gatcttgaaa ggctccggcg caaatttcat
     5521 catatccatg ttcttctcca ttaaaaaatc ctcctattat atagttttta tatagttttt
     5581 tgcagcgcaa cattacgctt tttgcttaat taataatatg tttttatgct tttttcttct
     5641 tataattagt ttcgtaatcc tttaaggcat tgatggcctg gccggagagt gggacgatcg
     5701 cgatcatatt aaatactgtc ataaggccta cgccaaggtc gccaagttcc cagacaaatt
     5761 cataggctgc cacgccgccg ataaatagca tgatcagtaa aaaaatcttg tatcctgtct
     5821 gagctttcca ggtatcgccg aatacgtaag atacgttgca gcgggcataa tagagaatgc
     5881 caaggaaagt ggaaaagctg aacaggaaga gtacaactgc gataaataca acgcctgcat
     5941 atccaaggtg atggttcata gctgcctgta aaaggtccat tcccataaga ccttcgatct
     6001 tttcagccgg agctaaaagc atcaggaatg cggaacagct gcagatcact aatgtatcga
     6061 taaaaacacc aagtgcctgg ataaggccct gttttaccgg atggctgact tctgctgcgg
     6121 ctgcagcaca tggagcagaa ccactacctg cttcatttga gaaaagtccg cgctttacgc
     6181 cgttcattac gattacacca agtccgccgc cggcaaactg tctgataccg aatgcctggg
     6241 agaaaatgtt tccaataaca gatggaagca gggtgatgtt cttaatgata acgaacagag
     6301 ttactaagaa gtaggcgcct gccataacag gaactaccat atctaaaacc ttaacagttg
     6361 cgttcttgcg aagaacgatg actgcggaca tagctaccag aatggtagca gttgcaacct
     6421 gcgggatacc gaatgcgttc tggaaggaag tggtaacgga gtttccgatt acctggctga
     6481 tacctgccca gcaaagcagt ccggagagtg cgaatgcggt tcctaaaaga ccgtatttac
     6541 aaccgcgggt gtaataagtc tgttcatcat ctgccacata ttcggcttct tcttttacgt
     6601 tctttacgaa aacatcttct cgtttgattt ttgtcaccag tcggatacgg tccattacat
     6661 aggcaggacc gccgcgataa ccgccgtaga gtgggtcttt ttctttgtag atctgtgcca
     6721 gggtagactc tacgaagctg ctggaagaac caatgagtgc ggtcagccac atccagaaaa
     6781 cagctcctgc gccgccaaat gagatagcag caacaacacc ggctaagtta cccataccta
     6841 cgcgggtagc ggtagcaacg atcagtgcct gaacaccgga tatagagttt tcatcttttt
     6901 ttgcttcgtc tttttccagt gtgacccgga tcatttccgg gaacaggcgg aatggaagaa
     6961 atttagtttt aaatgtaaag tataaacccg ctggaataag gatcaatacc agaagagaaa
     7021 gaccaaactg aacttctccc cctgagaatt ccaggataat taaatctccc cataacagat
     7081 cattgatagc tcccacaagt ttgatgatca tgtggagtat tcggttgatg agatacacct
     7141 ccttttgtaa tgtgtgaatt gaggttcacg ttgcgtcaaa ccgtcagggc cctctgacat
     7201 gtcttgcacg atcatacatc tgcagcaacc gctggcggct ggttctacag atgatgtttg
     7261 atgcgtgatg cgtgatgcat cacatatctt gtaattcata taatacacta ttttgcacaa
     7321 aagtcaatgg ttattttaaa aaaaaatatg caaaataaaa aagaagccga ccctgatggc
     7381 gcagaaggat gggacaggaa gaaaaaaaca ggaaatgtgt gacgggcgga tacacctgta
     7441 gttgtgatac ggaaaaagaa tgcgaggggc aattttgaaa atatattttg accgtttgct
     7501 aaaattgtag tatcatgaaa aaagtaagtg gatccatgcg tgatgcactg ataacagtac
     7561 aacagaataa agaaaataag aaaaacaatt aagaaaagaa ggaggcgggg gctatgcccg
     7621 aaaaaatcat taaacagaca ctcagtgaac agatctacaa cattctgaag gaagatattc
     7681 tgtctggtag gattttaatg ggcgataagc ttaccaatcg ggaacttcag gagcggtttc
     7741 aggtttcatc cacacccgtg cgtgacgcca ttaaccgact gtcaaaagat ggactgaccc
     7801 aggaagtatc aaaaagcgga gcacagctta tcacctttga ttattcctac gccaacgaac
     7861 tcaatgactt catcacattt ttagcctgta gggctgtgat gctttcttcc acagagcctg
     7921 atacagaaaa actggtagaa aagcttcaga tgtatgaaga agaaatggca ggagccaaaa
     7981 catccatgga atactttgag gcggattttc gctatcataa ggtattcttt gaccattgcg
     8041 gaaaccgttt tatgaaggaa acctataagc agtataattt tatccggacc cttcttatac
     8101 gttatgccat ccggaccgaa gatgaacggg atctggggat cagacagcac cgggggatca
     8161 ctcaggcttt tcagaaaaag gattatgaga aggcaagaaa gcttatggaa gaacattatc
     8221 tgtccggtat gcagttagca agagagcatt accgggaagg ggatactttc agagaaaata
     8281 aaaaatgatc agaaatattg ggaaacatta aggagacagt tatgagaaca gcaatcgcta
     8341 cagacagcaa cagcggtatt acacaggagc aggcagcaaa attaggtatt tatgtgctgc
     8401 ctatgccttt tatgatcaat ggggaaatgt tttatgaagg tgtgaacctg acccatgagg
     8461 aattttttaa acggatggaa gaaggggcag atatcactac ttcccagcct tccccaggtg
     8521 aggtaactga cttctgggat aagctgttaa aggaatatga tgaaatttta tatattccca
     8581 tgtccagcgg tttaagcggt tcctgccaga cagctatcat gctggcagat gattacgacg
     8641 gaaaagtaca ggtggtaaac aaccagcgta tttctgttac ccaggagcag tcagcactgg
     8701 atgcaaggga tttagctgca aagggatatg aggcagccca gattaaagaa atcttagaac
     8761 gtaccaagta cgagagcagt atttatatta cccttactac tttgaaatat ctgaaaaaag
     8821 gcggacgtat cactccggct gctgcagccc tgggaactct ccttcgcatt aagccggttt
     8881 taaccattca gggagaaaaa ctggatgcat tttcaaaggc acgtacagcc aagcaggcca
     8941 agaccattat gctcactgca ctggcaaaag acttagaaga gcgttttcat gacaagaatg
     9001 cggaacatac ctatctggag atcgcccata cctgcaatga agaaggggca aaagagctgg
     9061 aagaggcttt aaaagagcaa tatccaggtg tcccgatcac catagcacct ctttctttaa
     9121 gcattgcctg ccacattgga ccaggaagtt ttgcagtagc ctgctccagt aagctgaagg
     9181 agctggaata atttggaaaa agaaaaatgg tatgaaaagt taaaggcaca gggacagagt
     9241 attactgtcc ccatgactct tttgatgatc gcagtggctg ttatccctct tttattacag
     9301 acatggatcc tcatcggctc ttttaagcag aaccagatag aagccaggag cattgagata
     9361 cagaaccagt gtgtgatctt aagcaaccga atgacccgct ccgggtatat gacggcggat
     9421 aaggccaata atgtaagcct ggatgcccag atgcagacca tagcggatct gtataatggc
     9481 cgtatcgtta ttgtcaacag cagttataag atcatgacag atacttttaa tctgtctaca
     9541 ggaaaatatt atatatccga agaagttatc cgctgtttta aaggggaaag cggcagcagc
     9601 tttaataaag agggtggata tttttaccag actatccctg tttatgatcc ggcagatgag
     9661 aaaaagatcc agggagtgat cgcagtgact gcttccatgg aaaatgttgt gtcggtaaca
     9721 gaacgggtaa aaagcagtgc ccagctgttc tttctgtgta tggcactggt ggcaatcctg
     9781 ttttccatta tgtcagctca tttcctggtg cggcctttta agcggctcct gcgttccttt
     9841 gaccgggtgg cccaggggga tctggatgca gatatcagcg aagatgctta cagtgagacc
     9901 aggcagatgt cccaggcagt gcagaagtct cttcgcaagt taaaagcagt ggaccagtca
     9961 cgtcaggaat ttgtttccaa tgtgtcccat gagttaaaaa cccctattac atccatccgt
    10021 gtactggcgg actcccttat gggaatggaa gatgcgccgg tggaactgta cagggagttt
    10081 atggcagata tttcagatga gatcgaccgg gaaaatcaga tcattgagga tctgcttaca
    10141 ctggtgcgta tggataagtc cgcagaaagc cagatgaata tttcccaggt gcatatcaat
    10201 catgaactgg aaatgatctt aaagagactg cggcctattg ccaagagaag caatgtagaa
    10261 ctgatcctgg agagcatccg tgaagtaacc gcagatgtgg ataaagtgaa attaaacctg
    10321 gctgtgacca atctggtgga aaatgccatc aagtataata tagactccgg ctgggtccgt
    10381 gtaacactgg atgcagacca taagtatttt tatgttaaag tggcagactc cggcattggt
    10441 atcccggagg acagtgtgga ccatattttt gagcgctttt tccgtgtgga caaggcacgt
    10501 tccagggaag ttggcggtac aggactggga ctttcgatca caaaaaatgt gatccagatg
    10561 catcatggtg tgatcgatgt ggaaagtaca ccgggagagg gaactacctt cagtatgcgt
    10621 atcccgttaa attatgtgcc aagacaggag gaacggttat gaaacatgga aaaagaaagt
    10681 ttcttctgag tatactttta ggtgtactgg tggctgcaat ggcagtcctt tccggatgta
    10741 aagccaggac agaaccaaaa tcggatccgg gagccggaaa atacctgatc tattatctga
    10801 attcctcggt tacgaaactg gttgcccagg aatatgaaac agaaacaaaa gatcaggtgg
    10861 cgctggcaga ggaactggtc gatcagctga cttatgtgcc aagggatctg gactgccaga
    10921 ctgttttaaa tgataaagtg tcttttaaag cctgcaggat cgatgaacag gtactgtatc
    10981 tgtattttga cggcagttat acggctatga aaccggaaca ggagatcctt tgcagagcgg
    11041 cccttgcaag gactatgacc cagataccgg gaattgatta tatcagtatc tactgtgggg
    11101 accagccgct gatggaccgt cagggaaatc cggtgggatt tatctcagca ggtgatttta
    11161 ttatcaatac aagtaatgta aatgcctatg aaaagacgga actgaccctg tattttgcaa
    11221 acagcaccgg tgacaagctg gtggcagaaa agcgggaagt ggcccacaat atcaatacgt
    11281 ccatggagca gctgatcgtg gaacagctga tcgcaggacc atccgacagc agccatcagg
    11341 cagtactgcc atctgactgc aagatactta gtgtgtctgt aacggataat atatgctata
    11401 tcaattttga ctctgctttt ttaaacagca accaggcggt aaatgaatat atccctattt
    11461 acgccattgt aaattcactg gtagataaaa cctctgtttc taaggtacag atcatggtaa
    11521 acggttccca ggatgtaatg tttcgggaag tggtatcttt gaatacggcc tttgagcgga
    11581 atacggatta tattgagcag gaataaaggc ggatcaaaag cggaaagata aagcagaaat
    11641 taagaacaaa acaggagaaa acagaatatg aaacggcccc ttgtggtgat aactgcaggt
    11701 tttgtacttg gagaggtgct tgccctgtgt ttgcaggagg ctgtatacag aatatttttc
    11761 tggtttgcag gagtgctgtt tttgacagtg cttgctgtca ggcggttttt aagcaggagt
    11821 gtcatgtaca ggacgcttct gcttattttt gtgtttgttt ttggcgggat gacggcagga
    11881 agtttttcag gcgccaggtg ccgggaacag atggagcagg aagaaaagag gattatccag
    11941 gcaggatttg gggatgtggt gcagctgacc ggccaggtag tggatgtacg ggaaaaaagt
    12001 gaaaaactgg aaataattgt aaaccaggta aaagtgggaa agagagtgcg aaatattgct
    12061 gacccggatg acatccggtg gctgtcactt caatatccgg tgcagctttc tggaacccca
    12121 ggtgatctgg aaggctggga agaaaagctt cttccaggga aacaggtgaa aatggcctgt
    12181 tctttccgtc tcacagaacc tgcaaaaaat ccgggggaat ttgatgcaaa gctgtattac
    12241 cggtcaaagg gagtgatgtg taccggttac ataaaagctt taaaagaaag cagcggggcg
    12301 gaccggccgt ttatgagact tattctgtgg ttcagacgat attgcagtca tgttctggaa
    12361 gaatacggtg ggaaagaaga cagcagtatt tataaagcaa tgttactggg tgatacctcg
    12421 tttatggaga cggataccct ggatctgtac cgcagcagcg ggatcgccca tttgctggca
    12481 gtcagcgggc agcatttgtc actgatcggt ggtggaatgt atctgcttct tagaaagaca
    12541 gtgggaggtt tcagggcttc cgggataata ggaggcggtt ttgtgatcag ctatggcata
    12601 tttaccggct catccgggtc tgctatccgt gcagtcctta tgatctgctg tctgtggctg
    12661 gcagcagagc ggggacggac ctatgacagt ctttccgcct taagttttgc agcgttaggt
    12721 cttctgggaa acaatcccta tctgatcttt cacagcggtt ttcagctttc ttttgcagca
    12781 gtcctggcta tttccggacc ggggcagtgg atgatccggg agtataatat tggaaaaaac
    12841 tgggaaaaga cagtagtgat cagtttatgg gtgcagatct ttcttctgcc tgttatggca
    12901 tggcattatt accagtatcc tctgtatggg atgtttttga attttctggt attgccgctg
    12961 gtgccggttt tgatgatagc gggaatcatg atattgctta caggaggatt tttgcctttg
    13021 gcagcaggga cagctgctaa agtggggcat gtgatattgg gttattataa atggatctgt
    13081 gaaggttccc tgaaactgcc gggggcagta aggataatgg ggcggcccgg tccgggacag
    13141 atcctggggt atattgtgtt gtgggggata gcggtggctt tattgatatg gcagaggcgg
    13201 gggcagacca gaaagatcct gaaaaaatat ataaatttga tatttgcagt tgcaaccctg
    13261 ctcacagcag tattgtttag tttcgctatt ctttctctac acccggtccg tggttttgaa
    13321 cttctctgcc ttgatgtagg tcagggggat ggttttttgc ttaggtcagg aacaaccaac
    13381 atccttatag acagcggaag ttctgaccag aaaaaactgg gaagcaggac tcttgaaccc
    13441 tgccttaagt ctaaagggat ctcccgttta gatatagcag tcgtaagtca tggggacagt
    13501 gaccatataa gcggtctttt atattta
//
LOCUS       DBMDUA010000064        13416 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2435_length_13416_cov_3.182276, whole genome shotgun sequence.
ACCESSION   DBMDUA010000064 DBMDUA010000000
VERSION     DBMDUA010000064.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 13416)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13416)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13416
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2435_length_13416_cov_3.182276"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="ACZPCL_06640"
     CDS             <1..448
                     /locus_tag="ACZPCL_06640"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HIR1162178.1"
                     /translation="HNLILPENIPGVLQYIPPRILEQIPFISLTPGHGTYEFAREQFK
                     KFQISPSVSMELDNSPTVYRLVPQNNGFGFAPVTVTYEEKFDYTPIFCSMDHHVSSRE
                     IGLLYRDSSSLSPAARQFIQTAREVIPSFVKSEIPHFEVREDINFS"
     gene            501..1241
                     /locus_tag="ACZPCL_06645"
     CDS             501..1241
                     /locus_tag="ACZPCL_06645"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1162179.1"
                     /translation="MLSILYEDNHIIVVIKPAGLESQSGKSFAPDMVSEIRKHLASHP
                     QDIHKLTKETSTASKYPQPPYVGVIHRLDRPVSGIMVYAKNPKAAASLSTSLQQGKIE
                     KWYQAVICGKPVDNNGTYVDYLKQDKKNNCSRIVDKSDPESKRAELEYKVLDCIRTEE
                     KDSQYLSLVEIKLLTGRHHQIRVQFSGHGTPLYGDGKYGNNEKAVRKSLALCACHLKF
                     KHPATGKIMDFQIKPDTGAFKLFSKSND"
     gene            complement(1305..1763)
                     /locus_tag="ACZPCL_06650"
     CDS             complement(1305..1763)
                     /locus_tag="ACZPCL_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013696.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1162180.1"
                     /translation="MQVGTAVNIIAGQATMSMNARSYDMESREITKRKLYDIAEGMEK
                     ITDCKFDLEYVDGYEPIINDPGIVDLVISACRKNMGEDSVKIGEYDEGSDDFSYYMNA
                     TGTSGAYFFLLSGHEREECFVNHHPRFTWKEEAMKIGVESYLSVLMEYLN"
     gene            complement(1834..2028)
                     /locus_tag="ACZPCL_06655"
     CDS             complement(1834..2028)
                     /locus_tag="ACZPCL_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162181.1"
                     /translation="MGASALFGFPTTYILANEIANSVGENDAEKNYLRSHLQPHMVIA
                     GIVTVSISSVVFAGIVANWL"
     gene            2606..3277
                     /gene="trhA"
                     /locus_tag="ACZPCL_06660"
     CDS             2606..3277
                     /gene="trhA"
                     /locus_tag="ACZPCL_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIR1162182.1"
                     /translation="MSAKIKRNICRMKDPGSAITHLIAMVSAAIAAVPLLIKAAKTPG
                     YLHLAALAVFILSMICLYGASTLYHTLNISPVINKRLRKLDHMMIFVLIAGTYTPVCL
                     IVLGDHTGWNLLGLVWGIALVGIIINALWITCPKWFSSLIYIAMGWICILAISKIIAA
                     LPSGGFAWLLAGGIIYTVGGIIYALKLPIFNSRHRYFGSHEIFHLFVMGGSLCHYMMM
                     YQFVA"
     gene            complement(3262..3555)
                     /locus_tag="ACZPCL_06665"
     CDS             complement(3262..3555)
                     /locus_tag="ACZPCL_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR1162183.1"
                     /translation="MNYTYILHCADGTLYCGWTNDLKKRLHAHNSGTGAKYTKSRRPV
                     TLAYYEAFETKQEAMKREYAIKQLPRKKKEELISHGCNMDLCMVAEDQVYATN"
     gene            complement(3771..4664)
                     /locus_tag="ACZPCL_06670"
     CDS             complement(3771..4664)
                     /locus_tag="ACZPCL_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR1162184.1"
                     /translation="MKKIKGIMTGLLICVFLIMGSMAVWADQTGAVSGQTRVFDQAGL
                     FSETEKIQLEEEIAQCRKNTKMDVVVVSAYADDRTAEEYADDYYDYGGFGVGKKASGV
                     LLLYYMDGPGQSGGECYISTSGTMITLLTDERIETILDDVYGNLGNRDFAAASQIFLE
                     DVSDYVKQGVESGQYTYDRDTGEIVRYRSIRWYEAVAAVVIAGILAGSVCMDIKKRYA
                     MKQSSREAANSLQAYRADCAFHFSVTGDKLVNQFVRSIPIPRNTSGGSGRGHSGGSSS
                     GRSTVHTSSSGRSHGGGGRRF"
     gene            complement(4677..5795)
                     /locus_tag="ACZPCL_06675"
     CDS             complement(4677..5795)
                     /locus_tag="ACZPCL_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR1162185.1"
                     /translation="MGVITYKCPNCGGGLTFNAEKQKYKCEYCLSEFSQEELDEIEKR
                     ELEKARKQAEVQRDPEPEGAGSEAGTEEHVSGSKKTVTPIMYTCPSCGAQIVTDETTA
                     ASFCYYCHNPIVLEEKLRGEFKPDYVVPFQIDKKKAQEIFTQWVGRKKFVPDYFFSAS
                     QIESMTGVYFPYWLYSCQVKGDLQAEGKTVRVWDMAGMRYTETKVYDIRRQGDMNVDS
                     VARNALKKANRKLIDGVLPYRLNEKKDFSMGYLSGFMAENRDMEKDSFVNEVKEEVRQ
                     FALSSLSSQTGNYSSVQIRKNQADITGETWATALLPVWTVTYNDKARGKIYYFALNGQ
                     TGKIWGELPVDQKKLAVLFLSIFIPVCVILLFLGYWIG"
     gene            complement(5798..7084)
                     /locus_tag="ACZPCL_06680"
     CDS             complement(5798..7084)
                     /locus_tag="ACZPCL_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR1162186.1"
                     /translation="MGIIKAAAHAVGTGLADQWLEALEPDNMGAQTVFTKGVQIRKGK
                     GENGKGSDNMVTEGSIIHVYPDQFMLLVDGGKVVDYTAEPGYYKVSLDTQPSLMNGTL
                     GKAVKESLGRIRFGGIAPTAQRVYYINLQEIRGLKFGTKNAINYFDSFYNAELFLRAH
                     GTYSIKITDPLKFYAEVVPKNAERLEMEDINEQYLNEFLEALQTSINQMSADGIRISY
                     VPSKSMELGKYMANVLDEQWKELRGMEIQSVGIASISYDETSQELINMRNKGAMMGDP
                     SVREGYVQTTIAEGLKGAGTNSNGAMAGYMGMGFGMQNSGAFMGAASATNMQQMQMNR
                     QMQQGQQMPYGTGNGGFQSGQMGYGAANAGAQAVRPAQTANQPWICPACHVENTGKFC
                     SECGKPRPSVEWTCPNCGTVNKGKFCSECGSPRGEA"
     gene            complement(7085..8005)
                     /locus_tag="ACZPCL_06685"
     CDS             complement(7085..8005)
                     /locus_tag="ACZPCL_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1162187.1"
                     /translation="MKQNPVYNREMRVSSRSMKLPLIIFLFNGILFLVTLLNMYSVIM
                     QVKASASIQYSSFMELYEFVTSMEFILLMFIVPAVTASAISGERERQTLDLMLTTRMS
                     AGQIVTGKLLSALSTLFLLILSSFPAVAMVFVYGGITWTDAFSLILCYVTVAFFAGSI
                     GICFSAAFKRSTVSTVVTYGTLTAVVAGTYFLNRFALSVSGMDLQRSAAYVLGESSAK
                     ASSGGFFYLFLLNPAVTFMAVIGGQAGRGTPLADIVSYFGIPENGFIIKHWIGFSILI
                     QLAAGMALINRAIHFVEPIKHKKTSRKGKK"
     gene            complement(7983..8945)
                     /locus_tag="ACZPCL_06690"
     CDS             complement(7983..8945)
                     /locus_tag="ACZPCL_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1162188.1"
                     /translation="MLKIENLHKIYGKYHALSGLDMTVKEGALYGFVGPNGAGKTTAI
                     KIMTGLLQAEEGSVLINGKDALADPDQLKSCIGYVPDFFGVYDNLTVGEYMSFFASCY
                     QLDGLVARKRYTALLEQVGLEDKLDFYVDGLSRGMKQRLCLARALIHDPQILILDEPT
                     SGLDPRTRVEFKEILEDLKFAGKTILISSHVLSELSEMCTDIGIIDQGRMILEGNIQD
                     ILSRVNASNPLVISVYSNMEKALSILKSHPCVRTISMREKDICVRFTGDAQDEAILLQ
                     QLIDSDVLVNGFTREKGSLETVFMQLTDHEEERVVTSYEAESGL"
     gene            complement(8945..11542)
                     /locus_tag="ACZPCL_06695"
     CDS             complement(8945..11542)
                     /locus_tag="ACZPCL_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162189.1"
                     /translation="MDTGKNKMVGMRKKRALRGTLFAVILSASMSFSALAAMTADGPG
                     MGIAAGASADTSDSTDSEQVLKTEAAPEEGPIRMDVVYGYDNVAKSGRFLPLDIQLDN
                     TSEETFEGTLCVTSQEPDYDGYSNNSQEKYDTYRYEFPVMIQGDSSLEKKLNISLCSK
                     SDLMYITLMDGAGNEVLSKRLKMDLNTDAAQLFIGVLCDEPERLSYLEQVGVNYSMLR
                     TKTIDLSVYDLPDTELGLDQLDVLLITDFNSETLTEEQTEAIWEWVHRGGILLFGTGA
                     RGDETLSAFSKQLLEYPVSPGMVYEIQMGQDRTAREPGENILSLEGTEVDLKGGTDLL
                     TSGSLTVLSATSVGNGMAAAAIFDFTDLEEYCLKDNSYADYFLTALLGEDRINTLNSN
                     AGGGNYNQFWSVQSLVNTGNIRNLPKIGFYMTLAVAYIALAGPGLYFFLKQRDLREYY
                     QPAVALIAICTTGMVILMGTGTRFKGPFFTYATIENTSQTDKTETTFINMRAPYNKTY
                     SVELNPQYRLYPVTGSPYYDQNPSKEFFGEGEADITIRYGEDATWIQAVDTGAFNSKI
                     FQMELHTQNETGEGFSGEIRAFNGTVSGTLTNNYSYAVENAAILLYNQLLLIGNMEPG
                     ETISLDNRQVTFCATNFSYALASQITGVSLYDRNGDMEDATYVQALERANLLSFYIEN
                     YLSGYHAQASVVAFGRGKENLDFLKKNDYEAYGCTLLTSELDVNYEKGGNVYRSAMQK
                     QPNVLSGEYDADRNTMYGMAPVILEYYLGNETDVEALDFHPLTAEASDSLRYYYTVPF
                     TGGMYFYNYNTGDYDLMKPEKDHYTREELEEYLSPGNTITVKYVYDTTGEYTWNIMLP
                     VLTVTGRSK"
     gene            complement(11561..12709)
                     /locus_tag="ACZPCL_06700"
     CDS             complement(11561..12709)
                     /locus_tag="ACZPCL_06700"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1162190.1"
                     /translation="MKNDVSRRYRTRVITNIIYSAIITCLVEVFFVTNLSMVARYMGN
                     KGKMVLMVQAIEKQHTAVVLLYLILGILLFSITFMILQEPYIRYISKISDAVQNISEG
                     NLNTVIDVIGDDEFSGMAANLNRMAADIKQLMEKERESERTKNELITNVAHDLRTPLT
                     SIIGYLELLAGNRQIPEDMQHKYIEIAYGKSRRLEKLIEDLFGFTKLNYGKIAMHVGQ
                     LDIVKLLGQLLEEAYPNFVEKGLSYDLQSNVPAKTINADGNLLARLFDNLIGNAIKYG
                     ADGKRVLVQIHAESETVTVSVTNYGYVIPADELPLIFNKFYRVEQSRSSSTGGTGLGL
                     AIAKEIVDMHGGTISVASDLNGTVFTVKLQVRFDVNKENFGNIEDEKQ"
     gene            complement(12699..13403)
                     /locus_tag="ACZPCL_06705"
     CDS             complement(12699..13403)
                     /locus_tag="ACZPCL_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1162191.1"
                     /translation="MPDINILVVHDEKEIADVIEIYLVSDGYKVFKAENAEAGLSILD
                     KQDIHLVLLDIMMPGMNGLEMCRKIRETNNIPIIMLSAKSTDLDKILGLGTGADDYVT
                     KPFNPLELTARVKSQLRRYTQLNPNSGARETVNNEITIKGMSINRDNHKVIVDGEEIK
                     LTPIEFDILYLLASNPGKVFSTDEIFEKVWNEKVYEANNTVMVHIRRLRGKMKEDTRQ
                     NKIITTVWGVGYKIEK"
BASE COUNT         3512 a         3402 c         2577 g         3925 t
ORIGIN      
        1 tcataactta atacttccgg aaaacatccc gggagttctc cagtatattc ctccccgtat
       61 tctggaacag attcctttca tttccctgac accgggacat ggaacctatg aatttgcaag
      121 agaacaattt aaaaaattcc aaatatcccc ctctgtttct atggagctag ataattcacc
      181 taccgtgtac cgtttagttc cgcaaaacaa tggctttgga ttcgctcccg taactgttac
      241 ctatgaagaa aaatttgact atacacctat tttctgctcc atggaccacc atgtgagttc
      301 aagagaaatc ggattattat accgggatag ctcttcatta tctcctgcgg cccgccagtt
      361 tatccaaaca gccagagagg ttatccccag ttttgtaaaa tctgagatcc ctcattttga
      421 agtccgggaa gatattaact tttcttaaac tctattaaat gaaagtttaa aataataaca
      481 cctgaaaggt aaacccaaca atgctttcca tattatatga agataaccat attatagtcg
      541 taataaagcc agccggcctg gaatcacaaa gcggaaaaag ctttgccccg gacatggtca
      601 gtgagatacg aaaacacctg gccagtcatc cacaagatat ccacaaatta acaaaagaaa
      661 catccacagc ttcaaaatat ccacagccac cttatgtagg agttatccac agactagacc
      721 ggccggtatc tggtattatg gtatatgcca agaatcccaa ggctgcagct tcacttagta
      781 ccagccttca gcagggaaaa atagaaaaat ggtatcaggc tgtgatctgt ggaaaacctg
      841 tggataacaa tgggacatat gtggattatc ttaagcagga caagaaaaac aactgttcac
      901 ggattgtgga taagtctgat cctgaaagca aaagagctga gctggaatac aaagtgttag
      961 actgtatacg aacggaggaa aaggattccc aatacttaag tctggtggaa ataaagctgc
     1021 tcaccggcag gcatcatcag atccgggttc agttttccgg tcatggaacc cccctttatg
     1081 gagatggcaa atacggaaat aatgaaaaag ctgtaagaaa aagccttgct ttgtgtgcct
     1141 gccatttaaa atttaaacac ccggcaactg gcaagatcat ggattttcag ataaaaccgg
     1201 atacaggagc ttttaaatta ttcagcaaaa gcaacgacta atactaataa acgagctgtg
     1261 tacctctttt taccgtaggt aacacagctc gaaaactata atttctaatt cagatattcc
     1321 ataagaacag acaagtaact ctctactcct attttcatgg cctcttcttt ccaggtaaat
     1381 cgcggatgat ggtttacaaa acattcctct ctctcatgtc ctgaaagcag aaagaaataa
     1441 gctcctgaag ttcctgttgc attcatataa tagctgaaat catcacttcc ctcatcatac
     1501 tccccaatct ttaccgaatc ttcccccata tttttacggc aggcagaaat taccagatcc
     1561 acaattcctg gatcattgat gattggttca tatccatcta catattccag gtcaaattta
     1621 cagtccgtga ttttttccat tccttctgct atatcataaa gcttcctttt tgtaatttct
     1681 ctggattcca tatcatagga acgggcattc atgctcatag ttgcctgtcc ggctataata
     1741 ttgacagctg ttcctacctg caaggttcct acactgatga tttctgaatt tttaaagctt
     1801 cctgtttaat gtccataatc cattcctctc tttctatagc caattagcca caatacctgc
     1861 aaatactacg gatgaaatag aaactgttac aataccggca ataaccatat gtggctgaag
     1921 atggctgcgt aagtaatttt tttctgcgtc attttctcct acactattgg cgatctcatt
     1981 tgcaagaata taggttgtag gaaaaccgaa taatgcagaa gctcccaatg ctgtgcttta
     2041 acccgccata tccagacaat ggaacaggag cttggacatt ccctctttat caggcgaaaa
     2101 ggataccgca atgcacaact tacagatcag ggcgctgaat tttatcgcat tgcctggaat
     2161 aaagacttca aatcctggca ttctgaaaat tttgatgaaa ccataccacc actggttatt
     2221 ttagagcatg cagcccttgc agcctatttt atgacagaaa aatcctggac tttctgccct
     2281 tataccacag ccatacggct tcaaaaaaat ggagcttgca tttacgaact taagaattct
     2341 cctccagagc aggtggtata ttatctggta aatgaaaacc gaaaaacggc tacaatccat
     2401 aaatttctgg aacttttagc tgagaaactc aaaacacttc cgaaagataa gattacttct
     2461 tttctcagtt aaaacaaaca taaaagaaaa accttgcata atccttcgag cagttgtata
     2521 gtattaattg taacgataca tagttttgat tactacatgt aaaagttttc aaagcttata
     2581 tgacacatgc aaggaggtat ctgctatgag tgcaaagata aaaagaaata tatgccgtat
     2641 gaaagacccg ggaagtgcca ttacccatct gatcgctatg gtatctgctg ccatcgcagc
     2701 tgttcccctt ctgatcaagg cagcaaaaac gccaggctac ctgcatctgg cagcactggc
     2761 ggtgtttatt ttaagtatga tctgtctgta tggagcaagt accctgtacc atacactgaa
     2821 tatttcccct gttatcaata agcgtctgcg caaactggac catatgatga tcttcgtcct
     2881 tattgccgga acttataccc cggtatgcct gatcgtttta ggcgatcaca ccggctggaa
     2941 ccttttaggc cttgtatggg gcattgccct tgttgggatc atcatcaatg ccctgtggat
     3001 cacctgccct aaatggtttt cttccctgat ctacattgct atgggctgga tctgcatcct
     3061 ggcgatctcc aagatcattg cagcgcttcc gtcaggaggc tttgcatggt tacttgcagg
     3121 cggtatcatt tacacagttg gaggcatcat ctacgccctg aaactgccta tctttaattc
     3181 cagacaccgc tatttcggtt cccatgagat ctttcacctg tttgtcatgg gcggcagtct
     3241 gtgtcactat atgatgatgt atcagtttgt ggcataaacc tggtcctctg caaccatgca
     3301 cagatccata ttgcagccat gactgatcag ctcctccttc tttttccggg ggagctgttt
     3361 gattgcgtat tcccgcttca tagcctcctg ttttgtctca aaggcttcat aataggccag
     3421 tgtaaccggc cttctggatt ttgtgtattt ggctccggtg ccgctgttgt gggcatggag
     3481 ccgttttttt aagtcattgg tccatccgca gtacaatgtt ccgtctgcgc agtggagtat
     3541 ataggtataa ttcatgtctt tcccctgatg cactatctta tgatcttaca gaccccggtc
     3601 cctttcctct cctggtgccc actgggttat gtaccaggca gggcaaaaag ccctgccgtc
     3661 caggttccgg aacctgtaag atctatataa agcatcagcg gatgcgttac tatcattata
     3721 cgctgatgct tttaaactgt gtcagtcctt ttaaattttc cttctgaaaa ctaaaaacgc
     3781 ctgccgccgc caccatggct gcgtccgctg ctggaagtat gtactgtgct ccgtcctgat
     3841 gaactgcctc ctgaatgtcc tctgccgctt ccaccggaag tattccttgg aataggaatg
     3901 gagcggacaa actgattgac tagcttatca ccggtaacag aaaaatgaaa agcgcagtct
     3961 gccctgtatg cctgaaggct gttagctgct tccctgctgc tctgcttcat ggcatagcgt
     4021 ttttttatgt ccatacacac agagcctgcc agaataccgg cgatcacaac cgctgccact
     4081 gcctcatacc agcggatact gcggtaacgg acgatttccc cggtatcacg gtcataagta
     4141 tactgaccag actccactcc ctgtttcaca tagtcactta catcttccag aaaaatctgg
     4201 gaagctgccg caaaatccct gtttcccaga tttccatata catcatctaa gatggtttca
     4261 atgcgctcat ctgtaagcag ggtgatcatt gtccctgaag tagaaatata acattctcct
     4321 cctgactggc ccggtccgtc catatagtac agaagcagta caccgctggc tttctttccg
     4381 acgccaaatc cgccgtaatc ataataatca tctgcatatt cttccgccgt gcggtcgtct
     4441 gcataagcgc ttactacgac tacatccatt tttgtgtttt ttctgcactg ggcgatctct
     4501 tcctctaact ggatcttttc tgtttcactg aacagtcccg cctggtcaaa cacccgcgtc
     4561 tgaccgctta cagctcctgt ctgatctgcc cacactgcca tactgcccat gatcagaaat
     4621 acacagatca gcagaccggt cattatccct tttatctttt tcatctcatc acctcatcag
     4681 ccgatccagt atcctaaaaa cagcaatatc acacataccg ggataaaaat acttaaaaat
     4741 aaaactgcca gctttttctg atccaccgga agttcacccc atatctttcc cgtctgtccg
     4801 tttaaggcaa aatagtagat ctttcctctt gccttatcat tgtaagtcac tgtccacaca
     4861 ggaagcaggg ccgttgccca ggtctctcct gtaatatccg cctggttctt tcgtatctga
     4921 acgctggaat aatttcctgt ctgactgctg agactggata atgcaaactg ccttacttct
     4981 tctttcactt catttacaaa actgtccttt tccatatccc tgttttctgc cataaatccg
     5041 gaaagataac ccatggaaaa gtcttttttc tcattcaggc ggtaaggcag cacaccgtcg
     5101 atcagctttc gattggcttt ttttaaggca tttctggcta cagagtcaac gttcatatct
     5161 ccctgtctgc ggatatcata aacttttgtc tctgtatagc gcattcccgc catatcccac
     5221 actctgaccg tctttccctc tgcctgcaga tcacctttca cctggcagct atataaccag
     5281 taaggaaaat acacaccggt catgctctcg atctgactgg cagaaaagaa ataatcaggt
     5341 acaaattttt tccggcccac ccactgggta aatatttcct gagccttttt cttatcgatc
     5401 tggaaaggaa ctacataatc aggcttaaac tcaccccgca gcttttcctc caggacaatg
     5461 ggattgtgac agtagtagca gaaagaagcc gctgttgtct cgtctgtgac gatctgggcg
     5521 ccacagctgg ggcaggtgta catgatgggg gtaacagtct ttttggagcc tgatacatgt
     5581 tcctctgtcc cagcctcaga tcctgctcct tctggttcag gatcacgctg gacttctgcc
     5641 tgctttctgg ccttctcaag ctcccgcttc tctatctcgt ccagttcttc ctgtgaaaac
     5701 tcggaaaggc agtattcaca tttatatttc tgcttttctg cattaaacgt aagcccaccg
     5761 ccgcagttgg ggcatttata ggttatcact cccatactca ggcttctcct cttgggcttc
     5821 cacattcact gcagaacttg cccttattaa cagtaccgca gtttggacag gtccactcta
     5881 cggaaggcct tggttttcca cactcgctgc agaattttcc tgtgttttcc acatggcagg
     5941 caggacagat ccacggctga tttgcagtct gcgccggccg gactgcctgg gcgcctgcat
     6001 ttgctgctcc ataacccatc tgtccgctct ggaagcctcc atttcctgtt ccatatggca
     6061 tctgctgtcc ctgctgcatc tgccggttca tctgcatctg ctgcatattg gttgcggaag
     6121 cggctcccat aaacgcaccg ctgttctgca tgccaaatcc cattcccata tatcctgcca
     6181 tggcaccatt tgaattggtt ccagcgcctt tcagaccttc ggcaatggta gtttgtacat
     6241 aaccttctct tacggaagga tctcccatca tagcgccttt gttgcgcatg ttgatcagtt
     6301 cctgggatgt ctcatcgtag gaaatacttg caattcccac agactggatc tccataccac
     6361 gcagttcttt ccactgctca tccagtacat ttgccatata ttttcccagt tccatgctct
     6421 tggacggaac ataggaaatg cggataccat ctgctgacat ctggttaatg gaagtctgca
     6481 gtgcctctaa aaactcattt aagtactgct cattaatatc ttccatttcc aggcgttctg
     6541 catttttcgg aaccacttct gcatagaatt tcagcggatc tgtgatctta atggaatacg
     6601 ttccatgggc ccgtaaaaac agctctgcat tataaaaact gtcaaaatag ttgatggcat
     6661 tttttgtacc aaacttcagt ccccggatct cctgaagatt gatataatat accctctgtg
     6721 cagtgggggc aataccgcca aaacggatcc ttcccaggga ctcttttact gcttttccca
     6781 acgttccgtt catcagggaa ggctgtgtat ccaggcttac tttgtaatat ccaggctctg
     6841 ccgtataatc cactactttt ccgccgtcta ccaaaagcat gaactgatcc gggtatacat
     6901 ggataatgga accttctgtt accatgttat cggaaccttt tccattttcc ccttttcctt
     6961 tacggatctg aacgcccttt gtaaaaacag tctgggcccc catattgtca ggttctaagg
     7021 cctccagcca ctgatccgcc agtcccgttc ctactgcatg tgctgccgct tttataattc
     7081 ccatttattt tttccccttt ctactggttt tcttatgctt tatgggctct acaaaatgga
     7141 tcgcccggtt gatcagggcc attcccgctg ccagctggat caggatactg aaaccgatcc
     7201 agtgctttat gataaatcca ttttccggta ttccaaaata acttacaata tctgctaacg
     7261 gtgttcctct tcctgcctgt cccccgatca ctgccataaa tgtaacagcc ggatttaaca
     7321 aaaacagata aaagaaacca ccggaagaag ctttcgcact gctctcacct aaaacatagg
     7381 ctgcgctccg ctgcagatcc attcctgata cggaaagagc aaagcggttc agaaagtaag
     7441 ttcccgcaac cacagcggtc agtgttccat atgttacaac cgttgaaacc gtagatctct
     7501 taaaagctgc tgaaaaacag atgccaatac ttcctgcaaa aaaagctact gttacataac
     7561 ataggatcag agaaaacgca tctgtccagg taattcctcc atatacaaat accatggcaa
     7621 ctgccggaaa actggaaagg atcagcaaaa ataaggtact gagggcgctt aaaagctttc
     7681 ctgttacgat ctgtccggca ctcatccgtg tagtcagcat cagatctaag gtttgccgct
     7741 cccgttcccc gctgatcgct gaagcagtca ctgcaggaac gataaacatt agcaggataa
     7801 attccataga cgtaacaaat tcatataatt ccataaaact gctgtactgg atactggctg
     7861 acgcctttac ctgcatgatc acagaataca tatttaaaag agttaccaaa aacaagatcc
     7921 cattaaacag gaaaatgatc aggggcagct tcatgcttct ggaacttacc cgcatttccc
     7981 ggttatagac cggattctgc ttcataagac gtcaccaccc tttcttcttc atggtcagtc
     8041 agctgcataa agactgtttc caggctgcct ttctctctgg taaagccatt taccagcaca
     8101 tcactgtcga tcaactgctg cagcaggatc gcctcatcct gggcatcccc ggtgaagcgg
     8161 acacagatat ccttttccct catggaaata gtgcgcacac aggggtggct ctttaaaata
     8221 gacaacgctt tttccatgtt gctgtaaaca gagatcacca ggggattgga cgcattgact
     8281 cttgataaaa tgtcctggat atttccctct aagatcatcc ttccctggtc aatgataccg
     8341 atatccgtac acatttcaga aagttccgag agcacatggg agctgatgag aatggtcttt
     8401 cccgcaaatt tcaggtcttc cagtatctct ttaaattcca ctcttgtgcg gggatccagt
     8461 ccggaagttg gctcatctaa gatcaggatc tgaggatcat ggatcagcgc ccttgccaga
     8521 cacaacctct gtttcatacc tctggaaaga ccgtccacat aaaaatccag cttatcttcc
     8581 agacccacct gctccaaaag ggcagtgtaa cgtttccttg ccaccagtcc gtccaactga
     8641 taacaggaag caaagaaact catatattcc cctactgtca ggttatcgta aacaccgaaa
     8701 aaatcaggca catatccaat acaggacttt aactggtccg gatctgccag agcatctttt
     8761 ccgttgatca atacgcttcc ctcttctgcc tgtaaaagcc cggtcatgat cttgatcgcc
     8821 gtcgtctttc ccgctccatt gggaccgaca aatccataaa gagcgccttc ctttacagtc
     8881 atgtccagac cacttaaagc atgatatttg ccatatattt tatgcagatt ttcaatctta
     8941 agcattactt gctccttcct gtgactgtaa gtaccggaag catgatattc caggtatatt
     9001 ctcctgtggt atcatataca tacttcactg taatggtatt tcccggtgac agatattctt
     9061 ccagctcttc cctggtatag tgatctttct ccggcttcat cagatcatag tcccctgtat
     9121 tgtaattata aaaatacatg ccgcctgtaa atggaactgt gtaataataa cgcagactgt
     9181 cagaagcctc tgctgtcagc ggatggaagt ccaaagcttc cacatctgtt tcatttccca
     9241 gataatactc caagatcaca ggcgccattc catacatggt atttctgtcc gcgtcatatt
     9301 ctccggaaag aacatttggc tgtttctgca tggcagatct gtatacattt ccgccctttt
     9361 cataatttac atccagttct gaggtaagaa gggtacagcc ataagcctca tagtcatttt
     9421 tcttcaggaa atccagattt tcttttcctc ttccaaaagc aaccacactg gcctgtgcat
     9481 gataaccgga aagataattt tcaatataaa atgaaagcag attggcccgc tctaatgcct
     9541 gaacataggt ggcatcttcc atatccccgt ttctgtcata cagggaaacg cctgtgatct
     9601 gggaagccag cgcatagctg aaattggtcg cgcagaaagt cacttgacgg ttatctaagg
     9661 agatcgtctc acctggttcc atatttccga tcagcagcag ctgattgtaa agaagaattg
     9721 ctgcgttctc tactgcataa gaatagttat tggtaagagt accggaaact gttccgttaa
     9781 aagcccggat ctcacctgag aaaccttctc ctgtttcatt ctgggtgtga agctccatct
     9841 ggaaaatttt ggaattaaag gcccctgtat ccactgcctg gatccaggtg gcatcttccc
     9901 catagcggat ggtgatatct gcttctcctt caccgaaaaa ttccttcgat ggattttggt
     9961 cataataagg actgcctgta acaggataca gacggtactg gggatttaat tccactgaat
    10021 atgtcttatt ataaggagcc cgcatattga taaaagtcgt ctctgtttta tctgtctgac
    10081 tggtattttc tatggtagcg taagtgaaaa acgggccctt aaaacgggtt cctgttccca
    10141 ttaagatcac cataccagtg gtacagatgg cgatcagtgc cactgcaggc tggtaatatt
    10201 cccgcagatc tctttgtttt aagaagaaat aaagtcccgg acctgccagg gcaatatatg
    10261 ccactgccag ggtcatgtaa aatccgatct ttggaagatt gcgaatattt cctgtattta
    10321 ccagactctg gacagaccag aactggttgt aatttccacc acctgcgttg ctgtttaagg
    10381 tattgattct gtcttcacca aggagtgctg taaggaaata atctgcatag gagttgtctt
    10441 ttaaacaata ttcttccaga tccgtaaaat caaaaattgc tgccgctgcc attccatttc
    10501 ctacagaagt agcagaaaga accgtaaggg aaccacttgt aagcaggtct gttccgcctt
    10561 ttaaatccac ttctgttcct tccagggaaa gaatattttc ccccggttcc ctggctgtac
    10621 ggtcctgtcc catctggatc tcgtaaacca ttccaggtga aaccggatat tccagcaatt
    10681 gtttactgaa agcagataat gtttcatctc ccctggcacc tgttccaaac agtaaaatac
    10741 cgccacggtg tacccattcc cagatcgcct ctgtctgctc ttctgtaagg gtctctgagt
    10801 taaaatccgt gatcagaagc acatctaact ggtccagtcc cagttctgta tccggcagat
    10861 catacacaga caggtcaatg gtctttgtac gcagcatact gtaattgaca cctacctgct
    10921 ccagatagga aaggcgttcc ggctcatcac aaagtacacc gataaacagc tgggctgcat
    10981 ctgtgttcag atccattttc agccgtttgc taagtacctc attgccggcc ccatccataa
    11041 gtgtgatgta catcaggtct gacttgctgc aaagagagat atttaacttc ttttcaaggc
    11101 tgctgtctcc ctggatcatg accggaaatt cgtagcggta tgtatcatat ttttcctggg
    11161 aattgttgct gtaaccgtca tagtccggtt cctgggaagt aacgcacaaa gttccttcaa
    11221 aggtttcctc tgacgtattg tctaactgta tatccagtgg aagaaaacgc ccgctttttg
    11281 ccacattgtc atatccatag accacatcca tacggatagg gccttcctct ggcgctgctt
    11341 ctgttttaag cacctgctca ctgtccgtac tgtcggaagt atccgctgat gcccctgccg
    11401 ctatccccat tcccggtccg tctgctgtca tggcagccag agccgaaaaa ctcatggaag
    11461 cggacaatat gaccgcaaaa agggtgcctc tcagcgccct tttttttctc attccaacca
    11521 ttttattttt gcctgtatcc atcctgtaat tcctctttaa ttactgtttt tcgtcctcaa
    11581 tatttccaaa attttcctta tttacatcaa aacgaacctg aagttttaca gtaaatacgg
    11641 ttccgttaag atcactggct acactaatgg tccctccatg catatccacg atctcctttg
    11701 caatggcaag tcccagtccg gttcctcctg tactgctgga gcgggactgc tctacacggt
    11761 aaaatttatt gaaaataaga ggaagctcgt ctgccgggat cacatatcca tagttggtaa
    11821 cagaaactgt tactgtttcg ctttctgcat ggatctgcac caggacccgc tttccatctg
    11881 ctccgtattt aatggcattt ccgatcagat tgtcaaacag tctggctaaa agatttccgt
    11941 ctgcgttaat agtcttggca ggcacattac tctgcagatc ataagacaga cccttttcca
    12001 caaaattagg atacgcctct tccaaaagct gtcccaacag cttcacaata tcaagctgtc
    12061 ccacatgcat ggcgatcttg ccataattta acttggtaaa accaaacaga tcctcgatca
    12121 gcttctccag ccttcttgac tttccatatg cgatctctat gtatttatgc tgcatatctt
    12181 ccgggatctg acggttccct gccagaagtt ccagataacc gataatggaa gtaagagggg
    12241 ttcgcagatc atgggccaca ttagtgatca gctcgttttt cgtccgctcc gactcacgct
    12301 ctttttccat cagctgcttg atatccgctg ccatcctgtt taaattggca gccataccgg
    12361 aaaactcatc atccccgatc acatcgatca ctgtatttaa gtttccttct gatatattct
    12421 gcactgcatc agatatttta ctgatatagc ggatataggg ttcctgaagg atcataaatg
    12481 taatggaaaa cagcaggatc cccaggatca gatacaaaag caccaccgct gtatgctgct
    12541 tttcgatcgc ctggaccatc aggaccattt ttcccttgtt tcccatatac cgtgccacca
    12601 tagacaggtt tgtaacaaag aacacctcta ccaggcaggt aataatggcg ctgtatatga
    12661 tgtttgtgat caccctggtc cggtagcgtc tgcttacatc atttttcaat cttataacct
    12721 actccccaca cagttgtgat gatcttattc tgcctggtat cttccttcat ttttcctctt
    12781 aagcgacgga tatgtaccat aactgtattg ttggcttcgt atactttttc gttccacacc
    12841 ttttcaaaga tctcatcggt gctgaatacc tttccaggat tggaagctaa cagataaagg
    12901 atgtcaaatt caatgggtgt taatttgatc tcttctccgt ctacgatcac tttatgattg
    12961 tcacggttaa tgctcattcc cttaatggtg atctcattgt tcactgtttc ccttgcaccg
    13021 ctgtttggat ttaactgagt gtaacggcgc agctgggatt ttaccctggc tgtcagttcc
    13081 aatgggttaa atggctttgt cacatagtca tctgcacctg taccaagtcc taagatctta
    13141 tctaagtctg tggatttagc actgagcatg ataatgggaa tattgtttgt ctcccggatc
    13201 tttctgcaca tctccagtcc attcatacca ggcatcataa tatctaacag aaccaagtgg
    13261 atatcctgct tatccaggat actcagacca gcctctgcat tttcagcttt aaatacttta
    13321 tatccatcac tgaccagata gatctcgatc acgtccgcaa tctctttttc atcatgtaca
    13381 acaagaatat taatatcggg catttgattg tcctcc
//
LOCUS       DBMDUA010000065        13399 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2439_length_13399_cov_2.665363, whole genome shotgun sequence.
ACCESSION   DBMDUA010000065 DBMDUA010000000
VERSION     DBMDUA010000065.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13399)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2439_length_13399_cov_2.665363"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(414..1640)
                     /locus_tag="ACZPCL_06710"
     CDS             complement(414..1640)
                     /locus_tag="ACZPCL_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR1162192.1"
                     /translation="MKKNHLSSMTAATMAFLLAASAPFQAFAEEANPDSSYSVDQERL
                     TDNQLDYDEIGARVKEYYGPIKSAYNMVRGMTEDQGQIAVNERTMADDLISQARAAED
                     LAKDQTGMDQMINKATAKALRKTANQMRTMANNMDRSLSRKNSSEKQIDRQANSLILN
                     VEMMLNQYQQLQSQRTIAAKGVELATAAKQLQNTMQAQGMAVDADVLSAAASLSSGQS
                     QLNTLDAGIEQIRKMLCSFTGYDEASNPVFGEVPAADPSYIATVNVAEDKEKAVNNNY
                     ELISLRGQTGGGMTDLQIRTSKTTTQIANKLRNVEYSEENVRSNIQALYDAMEEKNSS
                     YSAAKTAYESGQISWQAAQVQKANGMLSNIQYMQQELAWLQAQSGYKCADLALQQAIQ
                     NYKWAVAGASVSADTQ"
     gene            complement(1721..2938)
                     /locus_tag="ACZPCL_06715"
     CDS             complement(1721..2938)
                     /locus_tag="ACZPCL_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR1162193.1"
                     /translation="MKNITKKITRISTVLTASILSAALPLTALAAQNSPDDNTYQPKD
                     VQTVDYKKSLTPEFAYSAEKWATLRDNIMEYGELADLIHEYNPTVLSNRSTYKDQKNK
                     NLNDIYDDYMKDIDDIWDQADNADNDVTWASLRYSAGLLTKQADNNYEDAEMEKIQYD
                     QQEAKLVYQAQEMMVSLEQSAYNLENLQSTRDLLQQQYEATQAQMSVGMATQTDVLTA
                     LKSVQDQDTAILTAKKSQENVHRNLCLMLGWSADAQPEIREVPQPDLSRVDALDPNAD
                     AQTAIDNNYDVRYYTKKAGNLTSQDLIESNQAAIVNAKDTAIRSLKTQYNTVLTTRDS
                     LNAAKAQLQVAEANLNLAQANLAVGSQTKLQYQSTLNTYTSAKNDVNTKELQLLLALE
                     AYDWNVKGLTTSN"
     gene            complement(2960..3598)
                     /locus_tag="ACZPCL_06720"
     CDS             complement(2960..3598)
                     /locus_tag="ACZPCL_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1162194.1"
                     /translation="MPTQRFLNLKDSKKNAILEAAAHEFARVPYSAVSINQIIKEAEI
                     SRGSFYTYFEDKDDLMRYMLRGFRDTLRKKVFEFLEEENGNPFRLCIRLLKDWIKTGS
                     ERITFQIHRNLLADLSVTGQNQMFGIRGFLMKDELYKSFVEELYNRLDKDRYPTTIET
                     LCYIVDMAIMILIKSMAMYYQNPSEKETIIQVLTEEMTILERGMCTQTSQQS"
     gene            complement(4152..4406)
                     /locus_tag="ACZPCL_06725"
     CDS             complement(4152..4406)
                     /locus_tag="ACZPCL_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162195.1"
                     /translation="MFARYIEIYTTLDMEKYYRVKNIFAENKIPYKDTSTNNQLRLSL
                     NNVRGTNPALLRDSGVKNTYSISVKKEDEYKARQLIQGRV"
     gene            complement(4491..5486)
                     /locus_tag="ACZPCL_06730"
     CDS             complement(4491..5486)
                     /locus_tag="ACZPCL_06730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1162196.1"
                     /translation="MNNAAENNTQKNNNGISQKDDRCCKLSVVVSVYNEEKALREFYK
                     ETNKILEQIKKSGWEHELIFVNDGSADNSLSILEEIAHEDHDVKLISFSRNFGHEAAM
                     IAGIDYSTGDGIICMDADLQHPPECIPEIIEKFNDGYEVINMVRTQNKSAGLVKNITS
                     SGFYWLINHISDVHFEPNASDFFAISRHVAQVLKDNYREKVRFLRGYVQNVGFKKTNI
                     EYEARARVAGESKYSIKKLFIFSINTILCFSNMPLKLGIYAGMLSASLGVAVMIYTLF
                     TRQGAPSGYATIVVLLCFMFAMMFLLIGIIGEYIAILFTELKDRPVYIVEKTENM"
     gene            complement(5486..6310)
                     /locus_tag="ACZPCL_06735"
     CDS             complement(5486..6310)
                     /locus_tag="ACZPCL_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HIR1162197.1"
                     /translation="MSRVHSANLKILKEIDRICRKYKIQYALDAGTLIGAVRHKGFIP
                     WDDDADIVFTRNQFEAFKKVVRRELPDTMELVEPDSFHEGKAFFDFTPRIIYKKSRMH
                     PDSNKMDYYDGKLNHLWVDLFILDKLPKGKAAAEITRFLHKAVYGLAMGHRHDLDYTK
                     YSLMYKVFVGGLATIGKFIPMKAIFFMQKAIALKDKHSKGDLRYYSNYQPDYLYVTLH
                     KDWCEEVIDAPFEDTTLMIPKGWDPVLKEVYGDYMQLPPEEKRVPTHSSLQIQVYD"
     gene            complement(6775..8121)
                     /gene="rfbH"
                     /locus_tag="ACZPCL_06740"
     CDS             complement(6775..8121)
                     /gene="rfbH"
                     /locus_tag="ACZPCL_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HIR1162198.1"
                     /translation="MFEHTTEAEARKQILDLVADYCDTFHKKKGYKEGDRIPYASRVY
                     DHSEMVNLVDSSLEFWLTSGRYTDEFEKKLAEYLGVRYCSLVNSGSSANLIAFMALTS
                     PLLGDRRVNRGDEVITVACGFPTTVAPMIQYGAVPVFVDVTIPQYNIDVNMLEEARSE
                     KTKAVMIAHTLGNPFDLRAVKAFCDKYNLWLVEDNCDALGTQYTMEEDGKEVVHFSGT
                     VGDIGTSSFYPPHHMTMGEGGAVYTNNPLLNKIIRSFRDWGRDCICPSGHDNLCGHRF
                     DRQYGELPVGYDHKYVYSHLGYNLKATDMQAAIGCAQLDKFPSFVERRRHNFARLKAA
                     LADCTDKLILPEAAPHSNPSWFGFLITCKEGTDRNAAVQYIESKGVQTRMLFAGNLIK
                     HPCFDEMRASGTGYRVVGELENTERILRDTFWVGVYPGMNDEMIDFMAKTIREAVR"
     gene            complement(8200..9264)
                     /gene="rfbG"
                     /locus_tag="ACZPCL_06745"
     CDS             complement(8200..9264)
                     /gene="rfbG"
                     /locus_tag="ACZPCL_06745"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HIR1162199.1"
                     /translation="MALTLEELKSFYNGKKVLVTGHTGFKGSWLVRILTLAGAQVTGY
                     ALNPPTDPNLFEIAGLEDTIHSVVGDVRDLAHLRRVFDEVKPEIVFHLAAQPIVRESY
                     KEPVYTYETNVMGTVNILECVRLTDTVRSFLNVTTDKVYENKEWEYGYRECDPLDGYD
                     PYSNSKSCSELVTHSYKKSFFEDGRCAISTSRAGNVIGGGDFASDRIIPDCVRAAAAG
                     KPIMVRNPHSTRPFQHVLEPLAVYLTIAMEQYKDSKYQDYYNVGPDDRDCVTTGTLTD
                     LFCNAWGEGMTWENHWVGGPHEANFLKLDCSKIKTVFGWKPRYTVKEAVEKTVEWSKE
                     YLNGADMLEVTDRQIREFFA"
     gene            complement(9274..10050)
                     /gene="rfbF"
                     /locus_tag="ACZPCL_06750"
     CDS             complement(9274..10050)
                     /gene="rfbF"
                     /locus_tag="ACZPCL_06750"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HIR1162200.1"
                     /translation="MKVVILAGGFGTRISEESHLKPKPMIEIGEKPILWHIMKYYSEF
                     GYHDFVICLGYKQYVVKEFFADYFLHTSDVTFDLANNSMEVHNNYSEPWKVTLVDTGL
                     NTMTGGRVKRIQPYIGNEPFMLTYGDGVSNVDLNELVAFHKSHGKIATITTVNLGQSK
                     GVLNIAEDNTVLSFREKDDNDGALINGGFMVMNPEIFDYLEGDSTIFEQGPMQKLAAE
                     GQMKSFYHGGFWQCMDTQREMKKLEELWQSGKAPWKIWKD"
     gene            complement(10113..11558)
                     /locus_tag="ACZPCL_06755"
     CDS             complement(10113..11558)
                     /locus_tag="ACZPCL_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162201.1"
                     /translation="MKLPGNRDKKIIDGTHRIVFYLLPLLGLAFLLWYIKNAACDVVY
                     SDYIRLVNSYLPDVFNPEKFFVADVLTRIPINYLSRIINVKFFGFSITFDRVLGAVSV
                     SLAAWCFAAYSRQLKINIKWFITFMIVMFSLNKWEMLTNGSGWSHFFAFACFYYHQIL
                     FDRYYRGQERKWDKTILMLLPWLIILGTAGPYCAIYAVVMILASGAAAFMDTGEKRKQ
                     YVIFLICTLIPLLLYILSNSFAVEEHAGATGRSLGEILKDFPTFPIRFILKSLAGMMV
                     GGEELQQWIANGCISNKIIYLLGVILMVGYLWSLWLNISLGIYKKTFFPLILLVSGGA
                     NHLLIFLSRYIFEKEDYALSSRYALQFQVGVLGILLTFALASQIRRKRINGGLVKRTV
                     PVLETVFCIAILIGNGYTTYREIQKAPYREENFEKMAEMAPQIPFMSDQELKEHSKEI
                     DDLYEYRKGTDKIRDAFRILEENHLNIFRDR"
     gene            complement(11590..13362)
                     /locus_tag="ACZPCL_06760"
     CDS             complement(11590..13362)
                     /locus_tag="ACZPCL_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162202.1"
                     /translation="MMLALAVWVNHGIQIKGLYMDDLYFWSCYGEQSFFEYVFPMGST
                     RFRFLYYLAAWLEMAVVRNHVALFVPINILLNAALSWYLYSIAWRLSRAKAIGFFTGA
                     MFLASRMAYYQIGQVLGLMETMALWMAISILWNLYRYVNEENREKCFYIASLLYFGVC
                     FVHERYMTLLPLLLLALLLKQCKKISLWLSTIVSFGLVQLIRAFTIGSVLPAGTGGTQ
                     VADTLNILQAIKFAFSQAAYVFGINAGPEHLNGCPWSESPMWVKGLIGMADGMLLIMV
                     LAFLVKFIMEKRKERKFSMLFNTMLFIGFIALNIASSSVTIRVEMRWIYVSLAGALLF
                     LSYIYGELTEGVKKELYLKRLYPWGILFALYVLLMLPAELFYRSCYPKLYLWPDQLRY
                     NSLAEQTYEKYGNGIFGKTIYIIGNSYEMSDFTARTFFKVFDPKMKAEGTKVEFIDSI
                     RDIGMVDDQMLVLREDPAHNAFQDITDFVRSLKLQTDYGYYSDGWMDEHASLTVMAGS
                     TGEIELEFMYPGVMSGGEAISITKDEEPVRTLPLHSSVVETTLQAEPWQMVHLQFDYN
                     FYMQNAREQRGQDRLAAIVHITTP"
BASE COUNT         3307 a         2983 c         2865 g         4244 t
ORIGIN      
        1 tacacttaaa tttttcattt cttcgtagtg ttttaaattt gctgtttttt tgtcatcggt
       61 tacgaaaatg tcacaatcaa aacggtttgc ggtctgaatc agataagaaa tcggtattgg
      121 attcagcgcc ttttttctta cgtccaaact cctggttacc attcacatca cctcaatttc
      181 agaaaactac agcatgttcc actccctcta ctgaattatt atattcccct tttgtttata
      241 atagcaccac tttttgttaa taacaaccag ttgttacaaa atttatcaat tttttatgtg
      301 caatagttat attttctttt gttttgataa ttaagaagcg acagttgtta cttttttgac
      361 aactgccgct ttttctttgg aagatttagt ttatttattt tgaaatatga tcattactgg
      421 gtgtccgcag atacggaagc acctgctact gcccatttgt aattctggat ggcctgctgg
      481 agagccaggt ctgcacattt atatccggac tgtgcctgaa gccatgccag ttcctgctgc
      541 atatactgga tgttgcttag catgccattg gccttctgca cctgcgctgc ctgccaggag
      601 atctggccgc tttcgtaggc tgttttggct gctgaatagg aggaattttt ttcttccatt
      661 gcatcataca gcgcctggat attggaacgg acattttctt cggaatattc tacattgcgc
      721 agtttattgg cgatctgtgt agttgtcttt gaggtacgga tctgcagatc tgtcattccg
      781 ccgcctgtct gtcctctgag actgatcagc tcgtagttgt tatttactgc tttttcttta
      841 tcttctgcta cattgactgt ggcaatgtag gatggatctg ctgccggtac ttctccgaat
      901 actggattag atgcttcgtc gtatcctgta aaggaacaga gcattttacg gatctgctca
      961 attcctgcat ccagtgtatt taactgggac tgaccggaag aaaggctggc tgctgcggaa
     1021 agtacatctg cgtcaactgc cattccctgg gcctgcatgg tgttctgcag ctgttttgct
     1081 gctgtagcca gttctacacc ttttgctgct atagtacgct gggactgcag ctgctgatac
     1141 tggtttaaca tcatttctac attaaggatc aaactgttgg cctggcggtc gatctgtttt
     1201 tcagaagagt tcttccggga taatgaccgg tccatattgt tggccattgt acgcatctga
     1261 tttgcagtct tacgcagcgc ttttgccgtt gccttgttga tcatctgatc catacctgtc
     1321 tggtctttgg caaggtcttc tgctgctctt gcctgggaaa taagatcgtc tgccatggtc
     1381 ctctcattaa ctgcgatctg cccctgatct tctgtcattc cccgtaccat gttgtaggca
     1441 cttttgatgg gaccgtaata ttcttttact ctggcaccta tttcgtcata gtctaactga
     1501 ttatctgtaa ggcgttcctg atcaacagaa tagctgctgt ccgggtttgc ttcttctgcg
     1561 aatgcctgaa aaggagcgga cgctgccagc aggaatgcca ttgtggctgc tgtcatggag
     1621 cttaaatgat tttttttcat cttttttgtc ctttctctct atgtactctc gaagtctttt
     1681 tgtatttgat cttgcgagaa agcctccggg agtgcatcta ttaatttgag gtggtaagtc
     1741 cttttacatt ccagtcatag gcctctaatg cgaggagaag ctgtaattct ttggtgttta
     1801 catcgttttt ggctgatgta taggtgttta atgtactctg gtactgcagt ttggtctggc
     1861 ttcctactgc aaggtttgcc tgggcaaggt ttaaatttgc ttctgctacc tgcagctgtg
     1921 ctttagctgc atttaaggag tctcttgtag taagtacggt gttgtactgt gtttttaagg
     1981 aacggatggc tgtatctttt gcatttacaa tagctgcctg gttggactcg atcagatcct
     2041 ggcttgttaa gttgcctgct tttttggtgt agtatcttac atcatagttg ttgtcaatgg
     2101 ctgtctgggc atctgcgttt ggatctaaag catctacacg ggataaatcc ggctgtggta
     2161 cttctctgat ctcaggctgt gcatctgcgc tccagcctaa cataaggcac agatttctgt
     2221 gtacattttc ctggctcttt tttgctgtta aaatggctgt atcctgatcc tgtacactct
     2281 ttaaagctgt aaggacatcg gtctgggttg ccattcctac ggacatctga gcctgggttg
     2341 cttcgtactg ctgctgcaga agatctctgg tactctgtaa gttttccagg ttatatgcgg
     2401 actgctctaa ggaaaccatc atttcctgtg cctggtatac cagttttgct tcctgctggt
     2461 catactggat cttttccatt tccgcgtctt cgtagttatt gtctgcctgt ttggttaaaa
     2521 ggcctgcgga gtaacgcagg gaagcccagg ttacatcgtt gtctgcgttg tcagcctgat
     2581 cccagatgtc atcaatgtct ttcatatagt cgtcatagat gtcatttaag tttttgtttt
     2641 tctggtcttt gtaagtggaa cggttgctta aaacggttgg gttgtattcg tggatcaggt
     2701 ctgctaattc gccgtattcc atgatgttgt cacgaagggt tgcccatttt tctgcggaat
     2761 aggcaaattc cggagttaaa ctttttttgt aatctactgt ctgaacgtct tttggctgat
     2821 aggtattatc atccgggctg ttctgggctg ccagagctgt aagtggaagg gctgctgaca
     2881 gaatggatgc tgtaagtaca gtggatatcc gtgtgatttt ttttgtgata tttttcattg
     2941 ctaagtctcc tcatttcgtt tatgactgct gtgaagtctg ggtacacatt cctctttcca
     3001 ggatcgtcat ttcttctgtt aatacctgga tgatggtttc tttttcggaa ggattttggt
     3061 aatacattgc catggatttg atcaggatca taattgccat gtctactatg tagcacaatg
     3121 tttctattgt ggtcgggtag cggtctttgt ctaagcggtt gtacaattct tctacgaagg
     3181 atttataaag ttcgtctttc atcagaaaac cacggatccc aaacatctgg ttctgtcctg
     3241 ttacgcttag gtctgccaga aggtttctgt ggatctggaa tgtaatacgc tcacttcctg
     3301 tttttatcca gtctttcaga agacggatac acaggcgaaa agggttgccg ttttcttctt
     3361 ctaagaattc aaatactttt tttcttaagg tatcacgaaa tcctctgagc atgtagcgca
     3421 tcaggtcgtc tttgtcttca aagtaagtgt agaaacttcc tctggatatt tcggcttctt
     3481 tgatgatctg gttaatggat actgcggagt agggaactct ggcgaattcg tgggctgcag
     3541 cttccaggat cgcatttttt tttgagtctt ttaagtttaa aaaacgctgg gttggcatgt
     3601 agttgcctcc ttgttggctt ttgtatggtt gatattattt gtatagatga caaggtgtta
     3661 tttgtgacat gttgtcaccg ttgtcacttt tgagattata tatatgttga ttgggaaagt
     3721 caataggaag aaggattaaa actttataaa caaacggcga aatggatggg atgggacacg
     3781 ggggatttgc gcggggacag actaaggatt tgagaaaagg gctaaaaaca agagaggggc
     3841 gggcaaaact cacttcgttc aaacagttgc ccgcccctct cttaacgccc ttttctcaaa
     3901 cccaagtctg tccagcctgc gcatcccatg tgtcccatcc aattcatttc gcctgagggg
     3961 gcctggtatc ctcattctcc cgccatcggc gggagaatat cgtcttagtt aaagattatt
     4021 tgcgcaagtg cgcattcttg cgacgaacgc ggtcgcttgc gacaaacttc ctcatctcat
     4081 gaatgtatat catttataaa aatgccccag ccaatgatgg ctgaggcatt tcccattatg
     4141 ttacagactg attatactct accttgtatt agctgcctag ctttgtattc gtcttctttt
     4201 tttacggata tagagtaggt gttttttaca ccgctgtctc gtaaaagggc aggattagtg
     4261 ccacggacat tatttaaaga cagacggagt tgattattgg tgcttgtatc tttatatggg
     4321 attttgtttt ccgcaaaaat gttttttaca cgataatatt tttccatgtc taatgtggta
     4381 tatatttcta tatatcttgc aaacatattt caccatcttt ccatatagcc cgtacatagg
     4441 acaccccaaa gacctttcct ctgcccctag aatggggcga aggaggtatc ttacatattt
     4501 tctgtctttt ctacaatata aactgggcgg tcttttagtt ctgtgaagag gattgcaatg
     4561 tattcgccga tgatccctat tagcaggaac atcatggcaa acatgaagca gagaaggact
     4621 actatggttg catagccgct tggagcacct tgtcgggtga aaagggtgta tatcataaca
     4681 gctaccccaa gggatgctga tagcatacct gcgtaaattc ccagttttag tggcatattg
     4741 gagaagcaga gtatcgtatt tatggagaat atgaacagtt ttttgatact gtatttgctt
     4801 tctcctgcta ctctggctct ggcttcgtat tctatgttgg tctttttgaa acctacgttt
     4861 tgtacatagc ctcgaaggaa acgaactttt tcacgatagt tgtctttcag gacctgggct
     4921 acgtgacggc tgatggcgaa gaagtctgag gcatttggct cgaaatgtac atcagagatg
     4981 tgattgataa gccagtaaaa gcctgaagat gttatatttt tcaccagccc cgcggacttg
     5041 ttttgtgtcc gtaccatatt tattacttcg taaccatcgt taaacttttc gatgatctct
     5101 gggatacatt ccgggggatg ttgaaggtct gcatccatgc agatgatccc atctccggtg
     5161 ctgtaatcga taccagcgat cattgctgct tcatgcccaa agtttcttga aaaactgata
     5221 agttttacat catggtcttc atgagctatt tcttccaaaa tactcagact attatctgca
     5281 ctgccgtcat ttacaaatat cagttcatgt tcccacccag atttttttat ctgttccagg
     5341 attttatttg tttctttata gaattctctt agtgcttttt cttcattata tacggatacg
     5401 actacagata atttacaaca tctatcgtct ttttgactta tgccattatt atttttttgt
     5461 gtgttatttt ctgctgcatt gttcattagt cgtatacctg tatctgtaaa ctggaatggg
     5521 ttggaacacg tttttcttcc ggcggaagct gcatatagtc cccatatact tctttaagga
     5581 ccggatccca gcccttcggg atcataagcg tcgtatcttc aaatggtgca tctattactt
     5641 cctcacacca gtctttgtgc aatgttacat aaagataatc cggctgataa ttgctgtagt
     5701 agcgcagatc acctttactg tgtttgtctt tcagggcaat ggccttctgc ataaagaaga
     5761 ttgctttcat aggaatgaat ttgcctattg ttgcaagacc gcctacaaaa actttataca
     5821 tcaggctgta ctttgtataa tccaggtcat gtcggtgtcc catggcaagg ccgtaaactg
     5881 ccttatgaag gaaacgtgtg atctccgctg cagcctttcc ttttggaagc ttgtccagga
     5941 taaacagatc tacccacaga tggtttaatt ttccatcata gtagtccatc ttattgctat
     6001 ccggatgcat acggcttttc ttatagataa tacgtggagt aaagtcaaaa aatgcttttc
     6061 cttcatggaa actgtctggt tctaccagtt ccattgtatc tggaagttct cttcttacta
     6121 cttttttaaa tgcttcaaac tgattacgcg taaaaacaat atctgcatca tcatcccagg
     6181 gaataaaccc tttatgacgt actgccccaa taagtgttcc cgcatccaat gcatattgga
     6241 tcttatactt ccggcaaata cgatctattt ccttaaggat cttaaggtta gccgaatgta
     6301 ccctactcat gtcatatcca acgttatcct ttatagcact aactttgtta ctaccattct
     6361 gcgcactatt tatgtcttcc accatgctat tcaccatatc atttaccgta tcacttacct
     6421 tattttctgc catatagcca tttcccctcc tttactaaac gcatatactt ttgcagccag
     6481 cgaagctggc tgcacatatt atttttcctc actcacgcct gcattgtaga actgagggaa
     6541 gggggacgct ttcatttttg caagtgtcca catcttgagc gcagctgtcc gaatgcagcg
     6601 gacatagtcc gatgcaggcg aacaggtgca tgccgaaagc cagtggacaa tgcaaaaatg
     6661 aaaaaaggac tcctttcctc agttcggacc cttcgcgaca tttccttggt cagaaaacct
     6721 ttcacacctc tcgcgacaga ctcttggcct gaaatcagct cagccctctc gcgactatct
     6781 taccgcctcc cgaatagtct tcgccataaa atcaatcatc tcatcattca ttcccggata
     6841 aactccaacc cagaaagtat cccgtaagat cctctccgta ttctccagtt cacccacaac
     6901 acgatacccg gttccggaag ctctcatctc atcaaagcac ggatgcttga tcagattacc
     6961 agcaaacagc atacgtgtct gtacgccttt gctttcaata tactgaacag ctgcatttct
     7021 gtctgttcct tccttgcagg tgatcaaaaa tccaaaccag cttggattgg aatgtggtgc
     7081 tgcttccggg aggatgagtt tatcggtgca gtcagccagc gctgccttta atcttgcaaa
     7141 gttatgacgg cggcgttcta cgaaagacgg gaacttatcc aactgggcac agccaatagc
     7201 agcctgcata tccgtagcct ttaaattata tccaagatgg gaatatacat atttgtggtc
     7261 atatcccact gggagttcgc cgtactgacg gtcaaagcgg tgtccgcaca ggttgtcatg
     7321 tccagatgga cagatgcagt ctcttcccca gtcacggaag gagcggatga tcttatttaa
     7381 aagtggatta ttcgtgtaaa cagcgccgcc ctctcccata gtcatatggt gaggaggata
     7441 aaaactggaa gttccgatat cacctactgt accgctgaaa tgtactacct cttttccatc
     7501 ttcttccatt gtatactgtg ttcccaaagc atcacagtta tcttctacca gccacagatt
     7561 gtatttatca cagaatgctt ttacagcccg caggtcaaat gggtttccta aagtatgagc
     7621 gatcatgact gcttttgtct tctcgcttct cgcctcttcc agcatattta catcaatatt
     7681 atactgtgga attgttacgt ctacgaatac cggtactgcg ccgtactgga tcattggtgc
     7741 tacggttgtt ggaaaaccac aggcaacagt aataacttca tcaccgcggt ttacacgacg
     7801 gtcaccaagg agtggagaag tcagtgccat aaatgcgatc aggtttgcgg aggaaccgga
     7861 gttgaccagg gagcagtaac gtacacccag atattctgcc agtttcttct caaattcatc
     7921 ggtataacgg ccggaagtca gccagaattc cagggaactg tctaccagat tgaccatttc
     7981 gctgtggtca tagactctgg acgcatatgg aatgcggtcc ccctctttgt atcctttctt
     8041 tttatggaaa gtatcgcagt aatcagctac cagatccagg atctgttttc ttgcttctgc
     8101 ttctgttgta tgttcaaaca tatttttatc tcctaatcaa ccaaattaac gtctttacaa
     8161 ttttgcaacc ggaacaggat aacgctcccg aaaaatacct taagcgaaaa attcccggat
     8221 ctggcggtct gttacttcta acatgtctgc accgtttaag tattctttgg accattctac
     8281 tgtcttttca actgcttctt ttactgtgta tcttggtttc cacccaaata cggttttgat
     8341 cttggagcag tctaatttca ggaaatttgc ttcgtgaggt ccgcctaccc aatggttctc
     8401 ccaggtcatt ccttctcccc aggcattaca gaataagtct gttaaggtgc ctgttgttac
     8461 acagtctctg tcatctggtc ccacattgta gtaatcctga tatttgctgt ctttgtactg
     8521 ttccatggca atggtcagat aaactgccag cggttctaat acatgctgaa acggtctggt
     8581 ggaatgtgga tttcttacca tgatcggttt tccggccgct gctgcgcgga cacagtccgg
     8641 aataatgcgg tcgcttgcaa agtctccgcc accgatcaca tttccggcac gggaggtgga
     8701 aatagcacag cgtccgtctt caaagaagga ttttttatag ctgtgtgtta ccagttcgga
     8761 acaggattta ctgttggaat aaggatcata gccgtctaac ggatcacact ctctgtatcc
     8821 gtattcccat tctttatttt cgtatacttt gtcggttgtt acgtttagga atgaccgtac
     8881 agtgtcagta aggcggacac attccaggat gttgaccgtg cccattacgt tggtctcgta
     8941 ggtgtagacc ggttctttgt aggactcgcg gacgataggc tgcgctgcca ggtggaatac
     9001 gatctccggc tttacttcat cgaacacacg gcgcagatga gccagatccc gcacatcacc
     9061 aactacactg tgaatggtgt cttccagacc tgcgatctca aagagattgg ggtctgtagg
     9121 cggatttaag gcatagcctg ttacctgggc acctgccagt gtaaggatgc gtaccagcca
     9181 ggaaccttta aatccggtat gtcctgtgac cagcactttt tttccgttat agaatgattt
     9241 taattcttct aatgttaatg ccataattac tccttagtct ttccagatct tccatggtgc
     9301 ttttccggac tgccacagtt cttccagttt tttcatttct ctctgtgtat ccatgcactg
     9361 ccagaaaccg ccatgataga aggacttcat ctggccttct gcagccagtt tctgcattgg
     9421 tccctgctcg aaaatagtag agtccccttc cagataatcg aagatctccg ggttcatgac
     9481 catgaaaccg ccattgatca gagcgccatc gttatcgtct ttttcacgga aggaaaggac
     9541 tgtattgtcc tctgcaatgt ttaacacgcc tttggactgt cccagattga cagttgtgat
     9601 cgttgcgatc ttgccatggg atttatggaa tgctaccagc tcatttaagt ctacgttgga
     9661 aacgccatcg ccgtaagtaa gcataaacgg ttcatttcca atatatggct ggatacgctt
     9721 tacacggccg ccggtcatag tgtttaaacc ggtatctacc agtgtgactt tccatggctc
     9781 ggaatagttg ttgtgtactt ccatgctgtt gtttgccagg tcaaaagtga catcggatgt
     9841 atgcaggaaa tagtctgcga aaaattcttt gactacatac tgcttatatc ccagacagat
     9901 cacaaagtca tggtatccaa attcggaata atatttcata atatgccaca ggatcggctt
     9961 ttcaccgatc tcgatcattg gctttggttt taaatggctt tcttcactga tcctggtgcc
    10021 gaagccccct gctaaaatta ctactttcat tttcaatctt tttcctccga gagtgtatct
    10081 atgttctccc acatttgtgg gaggatatat tgttaccggt ccctgaagat attcaggtgg
    10141 ttctcttcca ggatacggaa tgcatcccgg atcttatctg ttcctttacg gtattcgtaa
    10201 agatcatcta tttcttttga atgttctttt aattcctgat ctgacataaa tgggatctgt
    10261 ggtgccattt ctgccatttt ttcaaagttc tcttctctgt aaggtgcttt ctgtatctca
    10321 cgataagtag tatacccatt tccaattagg attgcaatac agaagacagt ttcaagaaca
    10381 ggcactgtcc gttttaccag tcctccattg atacgtttcc tgcgtatttg ggaagccagg
    10441 gcaaaggtga gaaggatccc caggactcct acctgaaact gcagggcata tcgggagctt
    10501 aaggcataat cttctttttc aaaaatgtaa cgtgaaagga agatgagaag atgattggct
    10561 ccgccggata caaggagtat taacggaaag aatgtttttt tataaatgcc aagacttatg
    10621 ttcagccaca gggaccacag atagccaacc ataagtatga ctcccagcag atagatgatt
    10681 ttgttggaaa tacaaccatt tgcgatccac tgctgtaatt cttcaccgcc aaccatcatt
    10741 cctgccaggg atttcaggat aaaacggatg ggaaatgtag ggaagtcttt taatatttcc
    10801 cctaaggatc ttcctgttgc ccctgcatgt tcttctactg caaaggaatt gctgagaatg
    10861 taaaggagca gcgggatcag tgtgcagata agaaagatca catactgttt tcttttttct
    10921 cctgtatcca taaatgctgc cgcaccggat gccagtatca taacaactgc atagatggcg
    10981 cagtagggac cggctgtccc cagtatgatc agccatggaa gcagcataag tatcgttttg
    11041 tcccattttc gctcttgtcc ccggtaatat cggtcgaaaa ggatctggtg ataataaaag
    11101 caggcaaagg caaagaaatg ggaccagccg cttccatttg tcagcatctc ccatttgttc
    11161 agactgaaca taacgatcat gaaggtgata aaccatttta tatttatctt cagctgacgg
    11221 gaatatgctg caaaacacca ggctgcaaga ctgacgctta cagcacctaa tacccggtca
    11281 aaggtaatgg aaaagccaaa aaattttacg tttatgatac gggacaggta attgatggga
    11341 atacgggtaa gcacatctgc cacgaagaat ttttcagggt taaatacatc tggcagatag
    11401 ctgtttacca ggcggatgta gtcggaatag actacatcgc aggctgcatt tttaatatac
    11461 cacaggagaa atgccagtcc cagaagaggc agaaggtaaa aaactatcct gtgtgtaccg
    11521 tcaattattt ttttatccct gtttccggga agtttcatgt gatctcctcc tgcgtatagt
    11581 tacgttcttt tacggtgttg taatgtgaac gatggctgcc agccggtcct gtcctctttg
    11641 ttctctggca ttctgcatat agaaattata atcaaactgc agatggacca tctgccatgg
    11701 ttctgcctgt agggttgttt ctactacgct ggaatgaaga ggcagtgtac ggaccggttc
    11761 ttcatctttt gtaatggaaa tggcttcccc gccgctcatt actcccggat acataaattc
    11821 cagttctatt tccccggtgg aacctgccat cacagtgagg gatgcgtgtt catccatcca
    11881 gccgtcggaa tagtatccgt aatcggtctg cagtttcagg ctgcggacaa aatctgtgat
    11941 atcctggaat gcattatgag caggatcttc tctgaggact aacatctggt catctaccat
    12001 tccaatatca cggatgctgt ctataaattc tacctttgtg ccctctgcct tcattttcgg
    12061 gtcaaatact ttaaagaagg tacgagccgt aaaatcgctc atttcgtagc tgttgccaat
    12121 gatataaatg gtttttccga aaatcccgtt tccgtatttt tcgtaggtct gttctgctaa
    12181 tgaattatag cgcagctgat ccggccacag atacaatttt ggatagcagc tgcggtagaa
    12241 cagttctgcc ggaagcataa gtaatacgta aagtgcaaag agaatgcccc agggatacag
    12301 gcgttttaaa tacagttctt tttttactcc ttctgtcagt tctccgtaga tataggaaag
    12361 gaagagcaat gcaccagcta gggatacata gatccaacgc atttccacac ggatggtcac
    12421 gctggaagat gcaatgttta atgcaataaa accgataaaa agcatggtat taaacagcat
    12481 ggaaaattta cgctctttcc gtttttccat gatgaatttt accagaaacg ccagtaccat
    12541 gataaggagc atcccgtctg ccataccgat cagccctttg acccacatag ggctttcact
    12601 ccatgggcag ccgtttaagt gttccggtcc tgcgttaatt ccaaagacat aggccgcctg
    12661 actgaaggca aatttgatgg cctgtaaaat atttaaggtg tctgctacct gggttccgcc
    12721 ggttcctgca ggaagaacag aaccaatggt aaaggcacgg atcagctgta ccagtccaaa
    12781 agagacaatt gtactgagcc agagggatat ttttttgcac tgttttaaga gaagggccaa
    12841 aagtaataat ggcagcagtg tcatgtaccg ttcatggacg aaacatactc caaaatacag
    12901 caggctggca atgtaaaaac atttttccct gttttcttcg tttacataac gatataaatt
    12961 ccagagaatg gaaatggcca tccagagagc cattgtctcc atcagtccta aaacctgacc
    13021 gatctgataa tacgccatcc ggctggctaa gaacatggct cctgtaaaga agccgatggc
    13081 ttttgccctg gaaagccgcc aggcaatgga atacagatac caggacagtg ctgcattcag
    13141 caatatgtta ataggcacga aaagagctac gtgatttctt accactgcca tttcaagcca
    13201 ggctgcaaga taatacagga agcggaaacg ggtgcttccc ataggaaata catattcaaa
    13261 gaaactttgt tcaccataac aggaccagaa atacagatca tccatataaa ggcctttgat
    13321 ctgtattcca tggttcaccc acacagctaa tgccagcatc ataaatcacc ctcaaattat
    13381 gcttcttagc tatagcatc
//
LOCUS       DBMDUA010000066        13254 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2467_length_13254_cov_2.728655, whole genome shotgun sequence.
ACCESSION   DBMDUA010000066 DBMDUA010000000
VERSION     DBMDUA010000066.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13254)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13254
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2467_length_13254_cov_2.728655"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(36..1424)
                     /locus_tag="ACZPCL_06765"
     CDS             complement(36..1424)
                     /locus_tag="ACZPCL_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR1162203.1"
                     /translation="MKDAYTKTPEQVLSDLHSDGSGGLTKEQAEQSRKKYGSNTFIRE
                     SHESLLKKIWDASTEPMLLMLIFAAVITLGVNIARYMTDGEYNFLECAGIFAAIALSV
                     VITIVTEGKSAKAFEALNRINEDTLVKALRDNSPQLIPQKDIVVGDILLVETGDKLVA
                     DARLLESNDLSTDESSLTGESLPAAKEADYVCPQSTPVAERRNMLYSGCFVSSGTGKA
                     VVTAVGNNTEFGQIAKELSSIEKDTTPLQEKLDRLGKVITVLGATAAFIVFAIQIIGF
                     AMSHTMNWETVSDAFITSIVLIVAAVPEGLPTIVAVSLALNIIKMSRENALVKKMIAC
                     ETVGCINIICSDKTGTLTENKMTVQQIYTKGELLEPEKLTDVCLLENFCINSNANVTI
                     EDGKDSFIGNPTECALLVAARKAGWDYTKKREEADIAHIFPFSSQKKDMSTILRTAEG
                     YMLYVKGNPEKS"
     gene            complement(1763..3595)
                     /gene="typA"
                     /locus_tag="ACZPCL_06770"
     CDS             complement(1763..3595)
                     /gene="typA"
                     /locus_tag="ACZPCL_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR1162204.1"
                     /translation="MKTKREDVRNIAIIAHVDHGKTTLVDQLLKQSGVFRANQEVQER
                     VMDSNDIERERGITILSKNTAVYYKDTKINIIDTPGHADFGGEVERVLKMVNGVVLVV
                     DAYEGPMPQTKFVLQKALELDLSVIVCINKIDRPEARPAEVIDETLELMMDLDASDEQ
                     LDCPFIYASARAGFARYELDDTNNDMEPLFETIVKHIPAPEGDPEAPTQVLISTIDYN
                     EFVGRIGVGKIDNGSLKVNQECVLVNHHDPDKFKKIKIGKLYEFDGLKRVEVQKAEIG
                     SIVAISGIADIHIGDTICSPENPEAIPFQKISEPTIAMYFMVNDSPLAGQEGKFITSR
                     HLRDRLFRELNTDVSLRVEETDSADCFKVSGRGELHLSVLIENMRREGYEFAVSKAEV
                     LYKYDERNRRLEPMELAYIDVPEEYTGVVIQKLTSRKGALQGMSQAAGGYSRLEFSIP
                     SRGLIGYRGDFMTDTKGNGILNTIFDGYAEYKGDIAYRQTGSLIAFEAGESITYGLFN
                     AQERGTLFIGPGVKVYSGMVIGQSPKAEDVEINVCKTKKLTNTRSSSADEALKLTPPK
                     IMSLEQCLDFIDTDELLEVTPESLRIRKKILDPTLRKRASFKNK"
     gene            complement(3709..4365)
                     /locus_tag="ACZPCL_06775"
     CDS             complement(3709..4365)
                     /locus_tag="ACZPCL_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HIR1162205.1"
                     /translation="MAENCKIIYKEGMGELVEKKSRFIAQIRPVKSEEEALAFVEETR
                     KKYWDARHNCYAYILKGEGATPTARCSDDGEPSQTAGKPMLDVLMGEGLMDVCAVVTR
                     YFGGTLLGTGGLVRAYSGAVKEGLANAQILEKKQGVRMEVITDYNGVGKIQYLAAQEG
                     IDTLDSQYTDKVVFIFLVPGEQEESFKAKLVESTNGRAVIRTDGEVYYGKCGGELILL
                     "
     gene            4664..7384
                     /locus_tag="ACZPCL_06780"
     CDS             4664..7384
                     /locus_tag="ACZPCL_06780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR1162206.1"
                     /translation="MNYGQYETERRLKSLSSKTGKYTSKLVFNIFKAFFILFLFLAVA
                     GAAVGFGMIKGIIDNAPSVDIMNIQPDRFATTVYDADGNLTETLVTSGSNRESATYEE
                     LPENLVNAFVAIEDSRFWTHDGIDLRSIARAVKGVVSDDYSGGGSTITQQLIKNNIFS
                     GGMETSWGARIERKLQEQYLAVQLEKNSGLNKEETKKTIITNYLNTINLGSNTLGVKV
                     AARRYFNKEVSDLTLAECTVLASITSNPSRYNPITNPENNNTRRQIVLQNMVDQNYIS
                     KQDMENALSDDVYDRIQNVNTATKETNSHYSYFTDELIEQVTEALMKKLGYTESQASN
                     LVYSGGLKIYTTQDPKIQAIVDEEVNDPGNYTVAKYSVEYRLSVNHADGTTQHYSEEN
                     LRAYRKNTLGDTSFEGLYNSKEEVQADIDRYKEWLLKDGDDVIAERQNLILQPQASFF
                     IMDQHTGEVKALSGGRGEKTASRTLNRATNVYRQPGSSFKVLTAFAPAIDTCGATLGT
                     VYYDAPYTIGTKTFRNWWGESRGFTGYSNIREGIIYSMNIVAVRTLMETVTPQLGVEY
                     AENLGITSLTKDDLGAALSLGGITKGVSNMELTAAYAAIANGGVYTTPRFFTKILDHD
                     GKVLIDNEPETKQVLKDSTAFLLTDAMSESMKANRKFARSGVSINSTSTRAALTGMSA
                     AGKSGTTTSNNDVWFVGFTPYYTAGVWGGCDNNQKLKHGGVDNGGTSFHKDIWRNIMN
                     RVHEGLEDPGFTVPDSVETAEICRKSGKRAVSGVCDHDPRGDAVYTEYFAKGTAPTEV
                     CDKHVAVTVCAESGKRSTEYCPTKTTKVCMVLPEGEEDLATDDSVFSIPGYCNIHGRN
                     STIISPTIEDNDNSGGETTTPTKATVVPVGPGYQPTTAPEWEYTGPGAKH"
     gene            complement(7491..7844)
                     /gene="spoVAE"
                     /locus_tag="ACZPCL_06785"
     CDS             complement(7491..7844)
                     /gene="spoVAE"
                     /locus_tag="ACZPCL_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HIR1162207.1"
                     /translation="MDYIRAFLAGGVICALVQILMEKTKMMPGRIMVLLVVSGAILGA
                     IGIYEPFKEWAGAGASVPLLGFGNTLWQGVKKAVGEDGILGAFKGGLTASSGGITAAL
                     VFGYLASLVFKPKMK"
     gene            complement(7907..8944)
                     /locus_tag="ACZPCL_06790"
     CDS             complement(7907..8944)
                     /locus_tag="ACZPCL_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HIR1162208.1"
                     /translation="MEQKKGRASILFEHPPVIKSVGTVSGTREGQGPLGRFFDSVEPD
                     DKMGMENWEEAESAMQEKAAGLALKKGKLTEQEMDYSFAGDLLGQLIATSFGSGSLHV
                     PLFGLYGACSTMGEALGLGAMTVNAGYGKNVLAMASSHFATAEKEFRFPLAYGNQRPK
                     SATWTVTGCGAAVIGKHGEKGIAQIAGITTGQVVDAGVRDSMNMGAAMAPAAFHTVMQ
                     NLKDLEITKEQLEGGWYDKIITGDLGKIGKQALADLLRNEGLEAEGFLMDCGVEIYDN
                     QKQDTHAGGSGCGCSALVLGALILPNIQAGIWKRVLFVPTGALLSTVSFNEGQTIPGI
                     AHGVILERMGK"
     gene            complement(9127..11244)
                     /locus_tag="ACZPCL_06795"
     CDS             complement(9127..11244)
                     /locus_tag="ACZPCL_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1162209.1"
                     /translation="MKFSIKHEINGRVRIHVFQRGVMSMRQADLLQYYLETLPGVLNA
                     KVYERTADAVIVYKGDRVEILEGVRTFSYDNEELEEIVPVNSGRELNREYQEKLFKHV
                     AVRMVTKTCFPFWLRAVYTTAKAIRYVFKGIRCLLKGKLEVEVLDATAITVSIIRSDF
                     NTAGSVMFLLGIGELLEEWTHKKSVGDLARSMSLNIEKVWQNVDGTEVLVPVSNIKEG
                     DLVTVHVGNVIPLDGEVVTGEAMVNQASMTGESVPVKKEAGSYVYAGTAVEEGEITLK
                     VKHAAGSTRFERIVQMIEDSEKLKSSVEGKAASLADALVPWSLGGTALTWLLTRNSTK
                     ALSILMVDFSCALKLAMPLSVLSAMREANSHHITVKGGKFLEAVSEAHTIVFDKTGTL
                     TKAEPVLADIVVFNDMTKNEILRIAACLEEHFPHSMANAVVKEAVNRKLVHEEMHSKV
                     DYIVAHGIATYVNGERVVIGSHHFVFDDEQCIIPDEHKQAFDELPPYYSHLYMAIGGK
                     LAAVILIEDPLRDEAKDTIDALRSLGVKNIVMMTGDSERTARAIAEKVGVDQYFSEVL
                     PEDKAGFVEREKALGHKVIMIGDGINDSPALSAADVGIAISEGAEIAREIADITISEE
                     NLYQLVTLKAISNGLMKRIHGNYRFVIGFNLGLILLGVAGVIAPATSALLHNTSTLGI
                     SLKSMTNLLNEQEKRKEYEKAEA"
     gene            complement(11312..11617)
                     /locus_tag="ACZPCL_06800"
     CDS             complement(11312..11617)
                     /locus_tag="ACZPCL_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HIR1162210.1"
                     /translation="MLDIGKVKKIGLFASGVLFGTAGVKVLGSKDAKKFYINCLAAGL
                     RAKDCVMTTATNIQENAEDILAEAKEINEQRNKEEVFEDESQAEESFKEEEAATAEA"
     gene            complement(11768..11980)
                     /locus_tag="ACZPCL_06805"
     CDS             complement(11768..11980)
                     /locus_tag="ACZPCL_06805"
                     /inference="COORDINATES: protein motif:HMM:NF015956.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HIR1162211.1"
                     /translation="MQALTEIKPGESCTVKWMVTGARIAEWMEKHSITEGSRIRVIQN
                     CMGDLIIGTEKEKVAVGREIAFRIKV"
     gene            12182..12904
                     /locus_tag="ACZPCL_06810"
     CDS             12182..12904
                     /locus_tag="ACZPCL_06810"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR1162212.1"
                     /translation="MKKPIIGILGAHMSNNGGPAPVPADYVNHAYCAGVENAGGIPIL
                     LPVLKDPADMDPVLAMCDGLLIPGGVDVDPRFYSEDPSPLLGSLDSAMDRFWIHAANY
                     ALEHKMPVLGICRGLQLVNVAFGGSLYQDLSYKNQDHMLHSQKQNRDYLIHQVTMDAD
                     SHLASILGAEPVYTNTMHHQCVKEPGKGLKITAHTADGIPEAMETDGGQFILVQWHPE
                     ELQDSEPRMRGLFKNLIEKASK"
BASE COUNT         3425 a         3324 c         2649 g         3856 t
ORIGIN      
        1 aatggtggct tgggctatcg gtgtcccgtg ctggtctatg acttttccgg atttcccttt
       61 acataaagca tataaccttc tgctgtacgc aggatcgtac tcatatcctt tttctgggaa
      121 gaaaatggga aaatgtgggc aatatctgcc tcttctcttt tctttgtata atcccagcct
      181 gcttttctgg ctgctactaa aagagcacat tccgtaggat ttccgataaa gctgtctttt
      241 ccgtcttcaa tcgtcacatt ggcattgctg ttgatacaga agttttccaa aagacagacg
      301 tccgtaagct tctcaggctc taacaattct cccttggtat agatctgctg tacggtcatc
      361 ttattttctg ttaaagttcc tgttttatcg gaacagatga tattaataca gccaacagtc
      421 tcacaggcga tcatcttctt taccagagca ttttcacggg acattttaat aatgtttaat
      481 gccagtgata cagccacgat ggtaggaaga ccttccggaa ctgctgctac gatcaggaca
      541 atactggtga taaaagcatc agacactgtt tcccagttca tggtatggga catggcaaaa
      601 ccgatgatct ggatggcgaa tacgataaag gctgctgtag cgcctaaaac tgtgatcact
      661 tttcccagac ggtccagttt ttcctgaaga ggtgtggtgt ctttttcaat tgaggataat
      721 tcttttgcga tctgtccaaa ctctgtgttg tttcctactg cagttaccac tgcttttcct
      781 gtaccgcttg atacaaagca gccggaatac agcatgtttc tgcgctctgc cacaggtgtg
      841 gactggggac agacgtaatc tgcctctttt gctgcaggaa ggctttctcc tgtaagagaa
      901 gactcatcgg tgctgaggtc attgctctct aataatcttg catctgctac cagcttatct
      961 cctgtttcta ctaaaaggat atcacctact acaatatcct tctgcgggat cagctgggga
     1021 gaattatctc ttaatgcttt tactagtgta tcttcattga tgcggtttaa tgcctcgaaa
     1081 gctttggcac tttttccctc tgtaacaata gtgatcacta cagataatgc gatcgccgcg
     1141 aaaataccgg cgcattccag gaagttatat tctccatctg tcatatagcg ggcaatattt
     1201 actcctaagg tgatcactgc cgcaaagatc agcatcaaaa gcataggctc tgtggaagca
     1261 tcccagatct ttttaagcag ggattcatga ctttcccgga taaaggtatt ggaaccatac
     1321 tttttgcggg actgctctgc ctgttctttt gtcaggccgc cgcttccgtc tgaatgcagg
     1381 tctgaaagta cctgttcagg ggttttggtg tacgcgtctt tcatttttct tctccttgta
     1441 ttttattgtt atcaataata ccagattgct ccgtgcttcg cactcacgca atcttcaaaa
     1501 atcctcctgc aagcaagctt gcatcggatt ttagaccttt tgaccctgta ttatcatatt
     1561 aaaaaacgag acatcatgta tggcaaataa gccatacatg agtctcgtta tttaagacaa
     1621 gccaggtcct tgaccagtat gttgacttgt cacactgccc ttacaggcag tgccactact
     1681 ccctcagttg ggtattatat tactcacgag ccccgcgcta cgcgctctac tgcctgctta
     1741 cgcaggcggc tcgcatatat ttctacttat ttttgaaaga agctctctta cgaagagttg
     1801 gatccaggat cttcttacgg atacgcaggc tctctggggt tacttccaga agttcatctg
     1861 tatcgatgaa gtccaggcac tgctccaggc tcatgatctt aggcggagtc agctttaatg
     1921 cttcatcagc gctggaagaa cgtgtgttgg tcagcttctt ggtcttgcag acattgatct
     1981 caacgtcttc agccttcggg ctctgtccga tgaccatacc agaatatacc ttaacaccag
     2041 gtccgatgaa cagagtacct ctctcctgtg cattgaacag accgtaagta atagactcac
     2101 cagcctcaaa tgcgatcagg gaaccggtct gacgatatgc gatatcacct ttgtactcag
     2161 cgtagccgtc gaaaattgta tttaagatac cattaccctt tgtatcggtc atgaaatcgc
     2221 ctctgtagcc gatcaggcct ctggaaggaa tggagaattc cagtctggaa tagccgccag
     2281 ctgcctggct cataccctga agagcaccct ttctggaggt cagcttctgg ataacaacac
     2341 cggtgtattc ttccggaaca tcaatataag ctaattccat tggctctaat ctgcggttgc
     2401 gctcgtcata tttgtaaaga acttctgcct tacttactgc gaactcatat ccctcacgac
     2461 gcatattctc gatcagaacg gacagatgca gctcaccacg accggatacc ttgaagcagt
     2521 ctgcagagtc agtttcctct acacgaaggc tgacgtctgt atttaactca cggaacagtc
     2581 tgtctcttaa gtgacgggag gtgatgaatt taccttcctg tccggctaac gggctgtcat
     2641 ttaccatgaa atacatagca atagtcggct cggagatctt ctggaatggg atcgcttccg
     2701 gattttcagg ggagcagata gtatctccaa tgtggatatc tgcaataccg gagatagcta
     2761 cgatagaacc gatctctgcc ttctgtactt ctactctctt taagccatcg aactcataca
     2821 gcttaccgat cttgatcttt ttgaacttat ccggatcatg atggtttacc agaacacatt
     2881 cctggtttac ctttaagctg ccgttgtcga tcttaccaac accgatacgt cctacgaact
     2941 cattgtagtc aatggtactg atcagaacct gggttggagc ttccggatca ccttccggag
     3001 ccgggatatg cttaacgatg gtttcaaaca gaggttccat atcattgttg gtatcatcta
     3061 attcatatct tgcaaaacct gctcttgcag atgcatagat aaatgggcag tctaactgct
     3121 catcagaagc gtccagatcc atcatcagtt ccagagtctc atcaataacc tctgccggtc
     3181 ttgcttccgg acggtcgatc ttgttgatgc agacgataac ggaaaggtct aattccagag
     3241 ccttctgaag cacgaacttt gtctgaggca taggaccttc gtaagcatcc actaccagta
     3301 caacaccgtt taccatttta agtacacgct ctacttcacc gccgaagtct gcatgtcctg
     3361 gtgtgtcgat gatattgatc tttgtatctt tgtagtatac agcggtattc ttggaaagga
     3421 tggtgatacc tctctcacgc tcaatatcgt tggagtccat tacacgttcc tgaacttcct
     3481 ggttggcacg gaatacaccg ctctgcttta acagctggtc taccagagtt gttttaccat
     3541 ggtctacgtg ggcgataatg gcaatgtttc ttacatcttc tctttttgtc ttcatagtga
     3601 ttatcttcct tttatatata cgaatctttt tgtctcagcc acaattttgt agtatagcat
     3661 tccatactcc atattgcaag gattttttca ttgtattttc tttgtacttt ataacaatat
     3721 cagttctccc ccgcactttc catagtacac ttccccgtct gtgcggatca ctgccctgcc
     3781 gtttgtgctt tccaccagct tggccttaaa actttcttcc tgctctcccg gcactaaaaa
     3841 gataaatacc actttatctg tatactggct gtccagtgtg tcaatgcctt cctgggctgc
     3901 aaggtactgg atttttccca ctccattgta atcggtaatc acttccatac gcactccctg
     3961 ttttttctcc aggatctgtg catttgcaag accttctttt actgccccgg aataagccct
     4021 taccaggccc cctgtgccaa gaagtgtgcc cccaaaatat cgggtgacta cagcacacac
     4081 gtccatcagt ccttctccca taagcacatc cagcataggt tttcccgctg tctgggaagg
     4141 ttctccgtca tcgctgcatc ttgctgtagg cgttgcccct tctcctttaa gaatataggc
     4201 ataacagtta tgccttgcat cccagtattt ctttctggtc tcttctacga aggccaaagc
     4261 ctcttcttct gactttacag gccgtatctg ggcaatgaac cgggattttt tctccaccag
     4321 ttctcccatt ccttccttat atataatctt acagttttcc gccactttat acgctcctta
     4381 taaaaacatt tataaaaaca ttcgtaatcc gctcattttt cttcaaaaaa tggcttctta
     4441 ctcatgtttt tggcgggacc caagttcaaa aatcctcctg caagcaagct tgcatcggat
     4501 ttacgacctt ttgtccctgg tatcatcata atatagttat cccactcttg caagcggcta
     4561 ttagtatccc ccttttcatt ttctgaaaat tgttgtataa tcgtaggagt ttaatcggct
     4621 attttctgtt caaaatgccg acacactgcg taaaggagac tatatgaact acggacaata
     4681 tgagactgag cgaaggctta agtccctttc atctaagacc ggaaagtaca catccaaact
     4741 tgtttttaat attttcaagg cattttttat cctgttcctt ttcctggcag ttgcaggtgc
     4801 tgcagttggt ttcggaatga tcaagggtat tattgataat gccccttctg tggatattat
     4861 gaacatccag ccggaccgtt ttgctactac ggtttatgat gcagatggaa atctgacgga
     4921 aactcttgtt accagcggct ccaaccgtga gtcagccact tacgaagaac ttcctgaaaa
     4981 tctggtcaat gcctttgttg ccattgaaga ctcccgtttc tggacccatg acggcattga
     5041 tcttcgttcc attgccagag ctgtaaaagg cgtagtcagt gatgattatt ccggcggtgg
     5101 aagtaccatt acccagcagc tgatcaaaaa taatattttt tccggcggta tggagacaag
     5161 ctggggcgcc cgtattgagc gaaagctcca ggaacagtac ctggcagtcc agctggaaaa
     5221 aaattccggt ttaaacaaag aagaaacaaa aaaaactatt atcaccaact atctgaacac
     5281 cattaacctg ggcagcaata ccctgggagt taaggttgcc gcacggcggt attttaataa
     5341 agaagtttct gaccttactc ttgcagagtg taccgtcctt gcatctatca cttccaatcc
     5401 ttcccgatat aatcccatta ccaatcctga aaacaacaat acaagacgtc agatcgtcct
     5461 tcagaacatg gtagaccaga attatatctc caaacaggat atggaaaatg ctctttctga
     5521 tgatgtatat gaccggatcc agaatgtaaa taccgctacc aaggagacca actcccacta
     5581 cagctatttt acagacgaac tgatcgaaca ggtgacagaa gccttaatga agaaattagg
     5641 ctacaccgaa agccaggcaa gcaatctggt atattccggc ggacttaaga tctacaccac
     5701 ccaggatccg aagatccagg caatcgtaga tgaagaagtc aatgatccag gcaattatac
     5761 agtcgctaaa tactctgtgg aataccgtct ttctgtcaac catgcagatg gcactaccca
     5821 gcattattca gaagaaaatt tacgtgccta tcgtaagaac actttaggag acacttcttt
     5881 cgaaggactt tacaacagca aagaagaagt ccaggcagat attgaccggt acaaagaatg
     5941 gcttttaaaa gacggggatg atgtgatcgc agaacgtcag aacctgatcc tgcagcctca
     6001 ggcatccttc tttatcatgg atcagcacac aggtgaggta aaagcccttt ccggcggccg
     6061 cggcgagaaa acagccagcc gtaccttaaa ccgtgctacc aatgtttacc gccagccagg
     6121 ttcttctttt aaagtactta ctgcatttgc tcctgctatt gatacctgcg gagccaccct
     6181 gggaaccgtt tactatgacg cgccttatac cattggcacc aaaactttcc gcaactggtg
     6241 gggagagagc cgtggtttta ccggttattc caacatccgt gaaggtatta tttactccat
     6301 gaacattgtg gcagtccgta ctttaatgga aactgttact cctcagcttg gtgtggaata
     6361 tgcagaaaac ctgggcatca cttctcttac caaagatgac ctgggcgctg ctctctcatt
     6421 aggcggtatt acaaaaggtg tttccaacat ggaactgact gctgcttatg cagctattgc
     6481 aaacggcggt gtttacacca ccccaagatt tttcacaaag atattagacc acgatggcaa
     6541 ggtgctgatc gacaatgaac cggagaccaa gcaggtatta aaggactcca ctgctttcct
     6601 gttaacagat gccatgtcag agtccatgaa agcaaacagg aaatttgccc gctccggtgt
     6661 atccattaac tccaccagta cccgtgcagc ccttacaggt atgtctgcag caggtaaaag
     6721 tggtaccacc accagcaaca atgacgtatg gttcgtaggt tttacccctt attacactgc
     6781 cggtgtatgg ggcggctgtg acaacaacca gaagttaaag catggcggcg tggacaacgg
     6841 cggaacctct ttccacaaag atatctggcg caatatcatg aaccgggtac atgaaggtct
     6901 ggaagatccc ggatttacgg ttcctgacag tgtggagact gctgagatct gccgcaaatc
     6961 cggaaaacgt gctgtatccg gcgtatgtga ccatgatcca agaggagatg ctgtctacac
     7021 tgaatatttt gcaaaaggaa cggctcctac tgaagtctgc gacaaacatg tggcagtaac
     7081 cgtatgtgca gagtccggaa aacgttccac cgaatactgc ccgaccaaga ccaccaaggt
     7141 atgtatggta cttccggaag gtgaagagga tctggctacg gatgactctg ttttctccat
     7201 cccaggttac tgtaacatcc acggccgcaa ttccaccatc atctctccta ccattgaaga
     7261 taatgataat tccggcggtg aaaccacgac tcctaccaaa gcaaccgtag tgcctgtagg
     7321 accaggctat cagcctacca ctgcgccgga atgggaatat acaggaccgg gagccaaaca
     7381 ttaagctttt aaactgcaaa cataaaaccg tgatctgttg actgcgcaaa aacagggact
     7441 gttgtatttg ataacacaat agtccctgtt tttttgatct gatccactct ttacttcatc
     7501 tttggcttaa ataccagcga tgccaggtat ccaaacacaa gggctgcggt aataccgccg
     7561 gaggatgccg taagcccgcc tttaaaagcg cccaggatac cgtcctctcc taccgctttt
     7621 ttcaccccct gccacagggt gtttccaaag cctaagagag gaacgctggc gcctgctccg
     7681 gcccattctt tgaagggctc gtagataccg atggcaccta aaatcgcacc ggatactact
     7741 aaaaggacca taatacggcc gggcatcatt tttgtttttt ccattaagat ctgcaccagg
     7801 gcacagatca cacctcctgc aagaaatgct cttatataat ccattttcac atcctgcctt
     7861 tcatacttct atctattttt acatcttttg ccttcgtaca aaaattttat tttcccatac
     7921 gttccagtat caccccatgg gcgatccccg gtatggtctg gccttcatta aagctgacag
     7981 tagaaagcag ggctcctgta gggacaaaaa gtacccgttt ccagatacca gcctggatat
     8041 ttggcaggat caaagcccca aggactaagg cgctgcagcc acagccgctt cctcctgcgt
     8101 gggtatcctg tttctggttg tcatagattt ctacgccgca gtccattaaa aatccttccg
     8161 cttccagacc ctcattccgt aaaagatctg ccaatgcctg ttttccaatt tttcccagat
     8221 ccccggtaat gatcttgtcg taccagccac cttccagctg ctcttttgtg atctccagat
     8281 cttttaaatt ctgcattacc gtatgaaagg ccgccggtgc catggcagca cccatgttca
     8341 tagagtctct gaccccggca tctactacct gccctgtagt gatccctgcg atctgggcaa
     8401 ttcccttttc cccatgtttc cctattaccg ccgccccgca gccggttaca gtccaggttg
     8461 ctgatttagg ccgctggttt ccataggcaa gaggaaaacg aaattccttc tctgccgttg
     8521 caaaatggct ggatgccatg gcaaggacat tttttccata tcctgcattt acggtcattg
     8581 ccccaagacc caaagcttct cccatggtag agcaggcacc atacaggcca aataatggca
     8641 catgcaggct tccggaacca aaggatgtag cgatcagctg ccctaaaaga tctcctgcaa
     8701 acgaataatc catctcctgc tctgttaact ttcctttttt caaagccaga cctgccgcct
     8761 tttcctgcat ggcactttcc gcttcttccc agttttccat tcccatctta tcatccggtt
     8821 ccacactgtc aaagaaacgt cccaaaggtc cctgtccttc cctggtacct gacactgttc
     8881 ctacactttt gataaccggt ggatgctcaa ataaaatgct ggcccttccc tttttctgct
     8941 ccatatccat tcatctgcct ttcttttctg caaacctctt ttctttagaa tgtgttatca
     9001 gcaggcaaat tatacagatc aaaatccggg aaaagcgagc acgcacatca ttggcacaaa
     9061 aaagaccgga acaaagtatt atttctaaca ccttattcca gtcttttcca gacagatctt
     9121 tatttattaa gcctctgcct tttcatattc ttttcttttt tcctgttcat ttaacaggtt
     9181 ggtcatactc tttaagctga tgcccagtgt agatgtattg tgcagcagcg cagaggtagc
     9241 aggtgcgatc acaccggcaa cacctaaaag gatcagtccc aggttaaatc cgatcacaaa
     9301 gcggtagttt ccatggatac gcttcattaa accattgctg atcgccttta atgttaccag
     9361 ctggtacaga ttttcctcag aaatggtaat atctgcgatt tctcttgcga tctcagcgcc
     9421 ttcactgatg gcgataccta cgtcagcagc agaaagtgca ggtgagtcat taattccgtc
     9481 accgatcata atgaccttgt gacctaaagc cttttcgcgc tctacaaagc cagccttgtc
     9541 ttccggaagt acctcggaga agtactggtc aacgccaact ttttcagcaa tggctcttgc
     9601 ggttctctcg ctgtcgccgg tcatcataac aatattcttg acaccaaggc ttcttaatgc
     9661 atcaatggta tcctttgcct catcacgaag aggatcctca ataaggataa cagctgccag
     9721 ctttccgccg atcgccatat acagatgaga ataatatgga ggcagttcat caaatgcctg
     9781 tttatgctca tccgggatga tacactgctc atcatcaaat acaaagtgat ggcttccgat
     9841 aacaacacgc tcgccattta cataagttgc aataccgtga gctacgatat agtcaacttt
     9901 ggaatgcatt tcttcatgga caagttttct gtttactgct tcttttacaa ctgcatttgc
     9961 catggagtga gggaaatgtt cctcaaggca ggcagcaatt cttaagatct cattcttggt
    10021 catatcattg aacacaacaa tatcagccag aaccggctct gcctttgtaa gagtaccggt
    10081 cttgtcaaat acgatggtgt gcgcctcaga tacagcctct aagaactttc cgcccttaac
    10141 agtaatgtga tggctgtttg cctcacgcat agcggaaaga acagataatg gcattgcaag
    10201 ctttaatgca caggagaagt ctaccatcag gatagacagt gcctttgtag agttgcgggt
    10261 cagcagccat gtaagagcag taccgccaag gctccacgga actaaggcat ctgccaggct
    10321 tgcagccttt ccttctacag aagattttaa cttttcagag tcttcgatca tctgtacaat
    10381 gcgctcgaaa cgggttgaac cagctgcatg ttttacctta agagtgatct ctccctcttc
    10441 tacggctgtt cctgcgtata cataagagcc ggcttccttc tttacaggaa cagactcacc
    10501 ggtcatggaa gcctggttta ccatagcttc gccggttaca acttcaccgt ctaacgggat
    10561 cacatttcca acatgaacgg ttaccagatc gccctcttta atattggata caggaactaa
    10621 tacttctgtg ccatctacat tctgccatac cttctcgata tttaaggaca tgcttcttgc
    10681 cagatcgcca acagacttct tgtgggtcca ttcctctaac agctcgccaa ttcccagtaa
    10741 gaacataaca gaaccggctg tattaaagtc acttcttata atagaaacgg taatagcagt
    10801 tgcatccaga acttctactt ccagtttgcc ctttaagaga caacggatac ccttaaatac
    10861 ataacggatg gcttttgctg tggtataaac tgctcttaac cagaatggga aacaggtctt
    10921 tgtaaccata cgtacagcta catgcttaaa cagcttttcc tggtactctc tgtttaattc
    10981 acgtccgctg tttaccggta cgatctcctc taattcctca ttatcataag aaaaggtcct
    11041 gactccttcc aggatctcca ctctgtcacc tttataaaca ataactgcgt ctgcggttct
    11101 ttcatatacc ttcgcattca gtactcccgg aagtgtttcc agatagtact gaagcagatc
    11161 cgcctggcgc atgctcataa cgcctctctg aaatacatgg atacggactc ttccgttaat
    11221 ctcatgttta atagaaaatt tcatacgtat atcgctccct gtaaaagaaa gcccgggaag
    11281 tttttctccc caggccccga ttagttttta attaagcctc agcggttgct gcttcttctt
    11341 ccttgaagga ctcttctgcc tggctctcat cctcgaaaac ttcttccttg tttctctgct
    11401 cgttgatctc cttagcttct gcaaggatat cttctgcatt ctcctggatg ttggttgcag
    11461 tagtcataac gcagtcctta gcacgaaggc cagctgccag acagttgatg tagaacttct
    11521 tagcgtcctt gcttcctaaa accttaacac cagctgtacc gaacagtaca ccacttgcga
    11581 ataaaccaat cttctttact ttaccgatat ctaacatcat atttttatcc tccttaattt
    11641 caggtatttt tataatttat tttttcattt gatactctaa caaatcaacc cacagttagt
    11701 caattccaac atttctcaat aacttacatt tttttgtcct gtcttttacc tcttctttac
    11761 atgaatttta tacctttatc ctaaaagcaa tctctcttcc taccgctact ttctcctttt
    11821 cagttccaat gatcagatcc cccatacagt tttgtatgac ccggatccgg ctcccttctg
    11881 taatactatg tttttccatc cattctgcta tccttgcgcc tgtcaccatc catttaacgg
    11941 tacagctctc tccaggcttt atttctgtta atgcctgcat atatatgccc cctaaatcaa
    12001 ttaatattga ctaacttaag ttattcataa ctaatattgt cagttaattt cctttgttgt
    12061 accaatattg tattcccata tcagccggtt agtcaattat aaattacttc tatttttcac
    12121 aaaataagtg taaaatataa atcagtgcat tgaaaaatac tacccaggag gatatataca
    12181 aatgaaaaaa ccgatcatcg gcatccttgg tgcccatatg tcaaataacg gcggcccggc
    12241 tccggtgcct gctgactatg taaatcatgc ttactgtgca ggtgtggaaa atgcaggagg
    12301 catccccatt cttctgcctg ttttaaaaga tccggcagat atggatccgg ttcttgccat
    12361 gtgtgacgga cttctgatcc caggcggagt agatgtggat ccacgttttt acagcgaaga
    12421 tccatctccc cttttaggtt ctttggactc tgccatggac cgtttctgga ttcatgctgc
    12481 aaactatgcc ttagagcata aaatgccggt ccttggcatc tgccgtggac tgcagcttgt
    12541 aaacgtagct tttggtggca gtctttatca ggatctttcc tataagaacc aggaccatat
    12601 gctccattcc cagaaacaga accgcgatta tctgatccat caggttacca tggatgctga
    12661 cagccattta gcttctattt taggtgcaga accggtgtat accaacacca tgcatcacca
    12721 gtgcgtaaaa gaacctggaa aaggtctgaa gattaccgcc catacagctg atggaattcc
    12781 cgaagcaatg gaaacagatg gcggacagtt tatcctggtg cagtggcatc cggaagagct
    12841 tcaggacagc gaaccaagaa tgcgtggctt atttaaaaat ctaatagaaa aagccagcaa
    12901 ataaatatgg ctgcgctgaa tgcgtattgt gaacatataa actaaaagca gcatacctgg
    12961 tcttatggat ctttcctaag attaacaggt acgctgcttt tttcacgttc catatccaat
    13021 tatactaaaa acagacacca tacaggtact gttaccagtg aaagcagtgt ggaagacact
    13081 acgcaccggg tagcaaattt ctcatccttt ttatatttgg acgctatgat cgctgttgtg
    13141 ctggctgcag gcaaaatatg tgcgacgtta tgattatgtg gtggcgaaag atgggagtgg
    13201 ggatttcttt acagttcagg aagcagtgaa tgcggcagtt ggtggtagaa aaca
//
LOCUS       DBMDUA010000067        13200 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2477_length_13200_cov_4.395603, whole genome shotgun sequence.
ACCESSION   DBMDUA010000067 DBMDUA010000000
VERSION     DBMDUA010000067.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 13200)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13200)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13200
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2477_length_13200_cov_4.395603"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..176)
                     /locus_tag="ACZPCL_06815"
                     /pseudo
     CDS             complement(1..176)
                     /locus_tag="ACZPCL_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203844.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            251..1042
                     /locus_tag="ACZPCL_06820"
     CDS             251..1042
                     /locus_tag="ACZPCL_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162213.1"
                     /translation="MKIIESKTEGKSPGRLSEDILVIMPDFIAVIDGVTPKSNFTYEG
                     KSTGRLAAELAGQAVRELKGTEDCQTFIKRCNQKFHDFYEKVDFPYDIRSKGLQVAAV
                     IYSEYLHEIWMIGDCQAMVDGREYLQPKRSDVILSQFRSLLMALQVPASEARAKVEPW
                     IVNATAFANKVGTSYGYSVLNGEEIPDELIKVIHLSEGKHEIILASDGYPLLRPTLQQ
                     SEQDLDRLLKEDPQCCRLYESTKGLKPGNKSFDDRTYVRFQAGTL"
     gene            1381..3042
                     /locus_tag="ACZPCL_06825"
     CDS             1381..3042
                     /locus_tag="ACZPCL_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1162214.1"
                     /translation="MKKSRKSFLLAMMAILVAAGLAGCGRGNSASGQQTENANGQTDQ
                     LAQAQTEDAEKDSVIVVMGPSSEPEAGFDPVYGWGAGEHVHEPLIQSTLTVTNKDLTI
                     GYDLATDMETSEDGMTWTVTIRSDVKFTDGEALTAEDVAFTYNTLRDNSTVNDFTMLD
                     EAEATDDTTVVFHMKRPYSIWPYTMAITGILPEHAYGPDYGQNPIGSGRYIMKQWDKG
                     QQVIFEANPDYYGEAPKMKHVTVLFMEEDAAFAAVQAGQADLAYTAASYSDLTVDGYD
                     LLAFKTVDNRGFNLPAVPSGAVTADGVAAGNDFTSDVRVRRAINLGIDREEMIQHVLN
                     GYGSPAYSVCDKMPWYNEASETSYDPEAAKTLLEEAGWTEGKDGIREKDGVQAGFTLM
                     YPASDSVRQALAADTANQLKELGIQVSIEGVGWDTAYDRAQSTPLMWGWGAHTPMEFY
                     NIYHTAQNSSFAEYSPYSNEKVDEYMDQALASPSLEESYDLWKKAQWDGENGVTQEGD
                     IPWIWLVNIDHLYWSRKGLHVADQKLHPHGHGWSIVNNVDQWTWE"
     gene            3156..4100
                     /locus_tag="ACZPCL_06830"
     CDS             3156..4100
                     /locus_tag="ACZPCL_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1162215.1"
                     /translation="MLILLFFVSIAAFALVSMAPLDPLQTNVGQAAMGSMSPEQIERL
                     RLYWGEGIPPLKRYLAWAGDFIRGNMGISLLYHQPVAKVIQVRFASSVWLMAAAWILS
                     GVTGVILGMTAGARKGSLTDRVISAYALVTASTPAFWVALVLLMVFAVWLKVLPVGLS
                     VPIGADAATVSIADRIRHGILPAAALSLTGISNITLHTREKMIQVMESDYVLFARTRG
                     ESMSSIVLRHGLRNMILPALTLQFASISEIFGGSVLVEQVFSYPGLGQAAVSAGLGSD
                     IPLLMGITMISAAMVFAGNFIADILYEIIDPRMRRGAK"
     gene            4195..4992
                     /locus_tag="ACZPCL_06835"
     CDS             4195..4992
                     /locus_tag="ACZPCL_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1162216.1"
                     /translation="MTALMALAVLVIGAVLVAGVCLKEQALTTDFTRKNIAPCIQYPF
                     GTDWMGRDMFVRTLTGLSLSIRIGLITSAFSSLIAVGMGLACAAGGKWADSLISGFID
                     LVMGIPHILLLILISFALGKGLKGVATGIILTHWTSLARLIRGEVLQLKESHFIKIAE
                     KMGMSPWQIGVKHMMPHLLPQVLVGMVLMFPHAILHEASITFLGFGLSPEQPAIGVIL
                     SESMKYLTMGKWWLALFPGILLVLVVMLFHVIGDTLNSLLDPSRAHE"
     gene            5136..6092
                     /locus_tag="ACZPCL_06840"
                     /pseudo
     CDS             5136..6092
                     /locus_tag="ACZPCL_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            6251..6850
                     /locus_tag="ACZPCL_06845"
     CDS             6251..6850
                     /locus_tag="ACZPCL_06845"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1162217.1"
                     /translation="MRLEAKDLSFRYHKGNRQILNHVSVSLDSSDRLGLAAPSGFGKT
                     TFCKILAGYEQPDEGQVLLDGKPLSEYKGYCPVQLIWQHPQEAVNPRRKMKTVLEEGG
                     VLDERLIHGLGIEKEWMERYPGELSGGELQRFCIARALGEKTSFLLADEITAMLDLIT
                     QSQIWNFLLEEVRSREIGLLVVSHSEPLLQRICTGRIQL"
     gene            6990..7736
                     /gene="larB"
                     /locus_tag="ACZPCL_06850"
     CDS             6990..7736
                     /gene="larB"
                     /locus_tag="ACZPCL_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HIR1162218.1"
                     /translation="MDVHKMLEQVKAGQLDIAEAEAKLKDLPYEDLGYAKLDHHRKLR
                     SGFGETIFCQGKPDPYLKEIFLKFYERDGEVLGTRASQAQYELIRSLVPAAVYDPISR
                     IVKVEKPDKELVGCVAVCTGGTADIPVAEEAAQTAEYFGTKVDRIYDVGVAGIHRLLS
                     QRERICKANCIIAVAGMEGALGTVIAGLADAPVIAVPTSVGYGASFHGLSALLTMINS
                     CANGISVVNIDNGYGAGYIATQINRMAVKK"
     gene            7736..9052
                     /gene="larC"
                     /locus_tag="ACZPCL_06855"
     CDS             7736..9052
                     /gene="larC"
                     /locus_tag="ACZPCL_06855"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HIR1162219.1"
                     /translation="MKKTLYLECNSGISGDMTVGALLDLGADRKVLEDALASLGVDGY
                     HLHFGTTEKCGIKAYDFDVHLEYEEHVHEHCHDHDHEHTHDHGDHDHDHGDHDHDHEH
                     THDHGHTHDHMHPHTHRNLYDIYTIIDRLQSSDKVKQLARRIFEIVAEAESKAHGLPI
                     DQVHFHEVGAIDSIVDIISTAVCLDNLGIEKVIVSPLAEGQGYVRCQHGTLPVPVPAT
                     ANIAQAYGLKLRFTETPGEMVTPTGAAIAAALSTGASMPKNYRIVKIGIGAGNKDFDH
                     ANILRAMLIEEEEEEKTDGLELLETNLDDCIGEALGYTMNLLLKAGANDVWYTPIYMK
                     KNRPAYMLSVLCRESNREVMEDIIFTHTTSIGIRRFPAERTALDRRKIQVETAYGTAD
                     VKVCSYKGKNYCYPEYDSVSALCDKQEKADFNTVYQAAAKAAKEQL"
     gene            9197..10036
                     /gene="larE"
                     /locus_tag="ACZPCL_06860"
     CDS             9197..10036
                     /gene="larE"
                     /locus_tag="ACZPCL_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HIR1162220.1"
                     /translation="MEGQNYSVKERLEQLEALMGKYTAGGKDLCVAFSGGVDSSLILK
                     AAVKAATKDGSKVYAVTFDSRLHPSCDLEIANRVAAELGGVHKVIHVNELEQEAIRMN
                     PVNRCYLCKKHLFVNLKEMAKSLGAETVMDGTNEDDHHVYRPGIQALKELGIVSPLAE
                     LHITKAEVKAMAKEWGISVASRPSTPCMATRLPYNTPIDYDVMKQIEVGEGYLRELLG
                     GNVRLRLHGNVVRIEADKTSFGKLLEHGDEIVKTLKNLGFVYITMDLEGFRSGSMDVG
                     LDK"
     gene            complement(10156..10797)
                     /gene="pcp"
                     /locus_tag="ACZPCL_06865"
     CDS             complement(10156..10797)
                     /gene="pcp"
                     /locus_tag="ACZPCL_06865"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HIR1162221.1"
                     /translation="MKLLLTAFDPFGGEPVNPALEAVKLVSDTVGSVQVVKLEVPTVF
                     GKSIDKVAKAIEAERPDAVLCIGQAGGRFDLTPERVAINLDDARIKDNEGNQPIDVTI
                     FEDGAPAYFATLPIKAMVQNMRNAGLPASVSNTAGTFVCNHLMYGVLYTLAKNYPGVR
                     GGFMHVPFIPSQVVNRPAAPSMNLQDIARGIEAAIAAIGENESDIVAAEGATH"
     gene            complement(10931..11863)
                     /locus_tag="ACZPCL_06870"
     CDS             complement(10931..11863)
                     /locus_tag="ACZPCL_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HIR1162222.1"
                     /translation="MTWNISTIVAEVFYCIIGLIFIATGMKALKDESCAHRTTTALFW
                     FILAFTFIAGPYLPKWITGLCVVFLAILTAFKRVAPSKSDMPKEAETRAAADRLGYSV
                     FIPPLVLAVSAVLVATFLKVLGANNAIGISGSIALVTAFIFFRPKAVYAVKDCTRLTD
                     NVGVTGILPQVLAALGSVFTAAGVGDVIAGGVSAIIPAGNLFAATAVYCIAMALFTMI
                     MGNGFAAFSVITVGIGIPFLIAQGADPVVVGALGLTAGYCGTLMTPMAANFNILPAAL
                     LETKNKYIVILSQLPVALVMLAVHIVLMYTLAFR"
     gene            complement(11865..12557)
                     /locus_tag="ACZPCL_06875"
     CDS             complement(11865..12557)
                     /locus_tag="ACZPCL_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008755316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HIR1162223.1"
                     /translation="MEIIKLIGVLIVVIGFILKLDTLAVVVVAGLATGLVAGMSPMDI
                     LNTLGTAFITNRTATLFILTLPVIGLCERNGLKDKAVDFIRSLKNMTTGKLIAIWQVI
                     RTFASAFSLRIGGHPQFIRPLINPMAQAAAVVQYKELDEKSEDQIKGMCAGSENYGNF
                     FAQNCFMGSSGTLLIVSTLSEQGYPVDALQIAGQSVPIAVISAVVGVIYALIFDQILK
                     RRLASKAAKEDK"
     gene            12941..>13200
                     /locus_tag="ACZPCL_06880"
     CDS             12941..>13200
                     /locus_tag="ACZPCL_06880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009216292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR1162224.1"
                     /translation="MKDWQYILKNLSLLSQMGISLAAPLLLCLFLCSWLNSRFMIGGW
                     IYIPGFIMGLGGSFMTAYKLYLAIMKTQKKKKNREHEKAFNR"
BASE COUNT         3900 a         2661 c         3445 g         3194 t
ORIGIN      
        1 ggaagggaac aaatttcatg ttgtggcaag tggtgcagac atacatggca ccgccaaaag
       61 aaggatcgcc acaattcagc attttatcga tattttccat ctcagaatct ctgggatgga
      121 gggtatattt gatttcctca taaatagtca gtaaagattc tttgcaagat attcataagt
      181 ctattatgta ggaaaagagg agaaaaagaa ggattttaat aatttagtat taatttcagg
      241 agaagatttt atgaaaatca tcgagtccaa aacagaagga aaatccccag ggcgcttaag
      301 tgaagacatt ctggtgatca tgccggattt cattgcagtg atcgatggag tgacaccaaa
      361 gtctaatttt acctatgaag gaaagtctac aggaagactg gcagcagaac ttgcagggca
      421 ggcagtgaga gagttgaaag gaacggaaga ctgccagaca tttattaaac ggtgcaatca
      481 gaagtttcat gatttctatg agaaagtgga cttcccttat gacatccgtt caaagggttt
      541 gcaggtggca gcagttattt attcggaata tttacatgaa atatggatga tcggggattg
      601 tcaggcaatg gtggatggcc gggaatatct gcagcctaag agaagtgatg tgatcttaag
      661 tcagtttcgc agtctgctga tggcattgca ggtacctgcc agtgaggcca gagcaaaagt
      721 ggaaccctgg atcgtaaatg ctacagcctt tgctaataaa gttggaactt cttatgggta
      781 ttccgtgctg aacggagaag agataccgga tgaattaata aaagtgatcc atctttcgga
      841 agggaaacat gaaatcatat tggcaagcga cggatatccg ctgttaaggc cgaccttaca
      901 gcagtcagaa caggatctgg acagactttt aaaggaagat ccccagtgct gcagattata
      961 tgagtctacc aagggactga aaccaggtaa taaaagtttt gatgacagga cgtatgtaag
     1021 atttcaggcg gggacattgt aaacggttta ttattgtaca tgcaatacat ggacagcagg
     1081 agtgtttcaa taagtaacac ttaaaaaggc tgcaatcggt tgacgaacaa ctgcttaggt
     1141 gttacaattc tatacagttt ccgacagaag ccggtggttc tcctgatgaa cgggagtgta
     1201 acaagaaaga tcataagata aaaaacagga tttataaaaa cagaattttg aaagataacc
     1261 ataccaggaa ggtgtgaatg ccataggaag gggcagttca taccttcttg ttttgtttat
     1321 tgagaaggta ccgaatgaag tattgatcaa attcagatat tttagaggag aacgaaaaat
     1381 atgaaaaaga gccgtaaaag ttttttattg gcaatgatgg caatcctggt ggcagcaggc
     1441 cttgcaggct gcggaagagg caacagtgct tctggtcagc agacagagaa cgcaaatggg
     1501 cagacggacc agctggcaca ggcgcagacg gaggatgcag aaaaagactc tgttatcgta
     1561 gtcatgggtc catcctcaga accagaggca ggttttgacc cggtttatgg ctggggcgca
     1621 ggggaacatg ttcatgaacc gctgatccag agtacattga ctgtgacaaa taaagatctg
     1681 accattggat atgacctggc tacagatatg gagacctctg aggatggcat gacctggaca
     1741 gtgaccatcc ggtcggatgt gaagtttaca gacggggaag cgctgacagc agaggatgtg
     1801 gcgtttacct ataatacgct tcgtgataac agtacagtaa atgattttac catgctggac
     1861 gaggcagagg ccacagatga tactaccgta gtattccata tgaaacggcc ttattctatc
     1921 tggccataca ccatggcgat cacaggtatc cttccggaac atgcctatgg ccctgattat
     1981 ggacagaacc ctattggttc cggccgttat attatgaaac agtgggataa gggacagcag
     2041 gtgatctttg aggcaaatcc ggattattat ggggaagcgc cgaaaatgaa gcatgtgacc
     2101 gttttattta tggaagaaga tgcggctttt gcagcagttc aggctggaca ggcagatctt
     2161 gcatacacag cggcttccta ttctgacctg acggtggatg gctatgatct gctggcgttt
     2221 aaaacagtag ataaccgtgg atttaacctt ccggctgttc cttccggcgc agtgaccgca
     2281 gatggtgtgg cagcaggaaa tgattttacc agtgacgtaa gggtgcgcag agccattaac
     2341 ttaggcatcg acagggaaga aatgatccaa catgtgttaa atggatacgg cagccctgct
     2401 tacagtgtct gtgataaaat gccatggtac aatgaggctt ctgaaacttc ctatgacccg
     2461 gaagctgcaa aaacattgtt agaagaagct ggatggacag aaggaaaaga tggcatccgc
     2521 gaaaaagacg gcgttcaggc cggatttaca ttaatgtatc ctgcaagcga ctctgtccgt
     2581 caggcgctgg cagcggatac agccaaccag ttgaaagaac ttggtatcca ggtatccatt
     2641 gaaggtgtag gctgggatac agcctatgac agagcccagt ccactcctct tatgtggggc
     2701 tggggcgccc acaccccaat ggaattttac aatatttacc atacagccca gaacagttcc
     2761 tttgcagagt attccccata ttccaacgaa aaagtggacg aatatatgga tcaggccctt
     2821 gcaagcccgt ctctggaaga gtcctatgat ctttggaaaa aagcacagtg ggatggagaa
     2881 aacggtgtga cccaggaggg agatatccca tggatctggc tggtaaacat tgatcatctc
     2941 tactggtcca gaaagggtct ccatgtagct gatcagaagc ttcatccaca tggacatggc
     3001 tggtcaattg taaataatgt ggatcagtgg acctgggagt agagatattg tggaaaacag
     3061 ttattgtgga aaaaagccgg atgtttacgg aaaatagtta ggagcagata agtttgaaaa
     3121 agcaggctat atttatcagt aaaaatttca tacggatgct gatccttctc ttcttcgtca
     3181 gtattgcggc ttttgccttg gtttccatgg cacctctgga cccactgcag actaacgtag
     3241 gtcaggcggc tatgggttcc atgagcccgg agcaaatcga gcgtctccgc ctttactggg
     3301 gcgaaggaat cccgccgtta aaacgctatc ttgcgtgggc tggggacttt atccggggaa
     3361 atatgggaat ttccctttta tatcatcagc ctgtggcaaa agtgatccag gtccgtttcg
     3421 ccagttctgt atggctgatg gcggctgcgt ggatcttatc cggagttacc ggtgtgatcc
     3481 ttgggatgac agcgggggca agaaagggaa gtctcacaga ccgtgtgatc tccgcctatg
     3541 cactggttac agccagtaca ccggccttct gggtggctct ggtgcttctt atggtgtttg
     3601 cagtgtggtt aaaagtcctt cccgtaggtt taagcgttcc cataggcgca gatgcagcca
     3661 ccgtttctat agcagaccgc atccgtcatg gtatattgcc ggcagcggct ttaagtctta
     3721 caggcatttc caatatcacc ctccatacca gggagaaaat gatccaggtt atggaaagtg
     3781 attatgtgct ttttgcaaga accagagggg aaagcatgag ttccattgtt ttaagacatg
     3841 ggctccgcaa catgatcctt ccggccctga ccctgcagtt tgcttcgatc agcgagatct
     3901 tcggcggctc cgttctggtg gaacaggtat tttcctatcc gggactggga caggcggcag
     3961 taagcgcagg tcttggcagt gatattccgc tgcttatggg aattaccatg atcagtgcag
     4021 cgatggtgtt tgcaggcaat tttattgctg atattttata tgagatcata gaccccagaa
     4081 tgagaagagg cgcaaaataa aatcgcaaaa ccttgaaaat gggggatgaa aggtaatgat
     4141 aatttgaaga agtatttgaa acggacagag gggataagca gcaggcgtgt gcttatgacg
     4201 gcgcttatgg cactggcggt attagtgatc ggggcagtgc tggttgcagg cgtctgtctg
     4261 aaggaacagg cactgactac ggattttacc aggaaaaata tagcaccctg tatacaatat
     4321 ccttttggaa cagactggat gggccgggat atgtttgtgc gtacccttac aggtttgtcc
     4381 ttaagtatac gcattggcct gattacatct gccttcagct ccctgatcgc agtgggaatg
     4441 ggacttgcct gtgcggcagg cggaaaatgg gcagacagcc tgatcagtgg ttttattgac
     4501 ctggtcatgg gaatacccca tattctgctt cttatcctga tctcttttgc attgggaaaa
     4561 ggtttaaaag gcgtggcaac aggcattatc ctgacccact ggacttcttt agcacgattg
     4621 atccgcgggg aagtgctcca attaaaggaa agccatttta taaagatcgc tgaaaaaatg
     4681 ggtatgagtc catggcagat aggtgtcaag catatgatgc cccatctgtt gccacaggta
     4741 ttagtgggaa tggtacttat gtttccacat gctatactcc acgaagcaag cattacattt
     4801 ttaggttttg gactatcacc ggagcagccg gctatcggtg tgatcctttc tgaaagcatg
     4861 aaatatctga ctatgggaaa atggtggctg gcgctgttcc cgggaatact attggtgctg
     4921 gtagtcatgt tatttcatgt gatcggtgat acgttaaaca gtcttttgga tccgtccagg
     4981 gcacatgagt agggaaaatg agtataaaaa gcaaaagata gatgctgtta aaagatgatc
     5041 atgaagaaaa acgcaggaaa agcataaaaa gagaacgcag gaaagtgcgg gaaaaaggtg
     5101 gaaaggaggc gccgatgcag atggaaaaca taaagatgta tgagaaaaca gaaaaatctg
     5161 aaaaaacagg aaaacagaaa aagtttgaaa gagaagaact tcttcgcatc aaacatttat
     5221 ccgttacatt tacccaatat gacggctggt tttcaagaaa aacactgcct gtgatccgtg
     5281 atctgagtct cagtgtttca cggggagaaa tggtagctgt ggttggttcc agcggctccg
     5341 gaaaaagcct tttagcccat gcagtcatgg gggttctgcc ttataatgga aaatgtgggg
     5401 gagatattta ttataaaggc gagcaattga cctcaaagcg gatcaaaaag ctgcggggcc
     5461 atgagatcgt cctggttccc cagagtgttt cttatctgga cccattgatg aaagtgggag
     5521 agcaggtagc aggcgggaaa gaacggatca gtttggaaaa aagccggaag gcactggccc
     5581 ggtatggcct tgataaagaa gtggatcata tgtatccttt tgaactttcc ggcggaatgg
     5641 caaggcgggt gctgatcgga acagcggtgg tggaacagcc ccagctggtg atcgcagatg
     5701 aacctacgcc tgggcttcac atggaagcgg ccctgcgggt gctgtcccat ttccgtgaga
     5761 tcgcagatca gggggcagga gtgcttctga tcacccatga tctggagctg gcgttaaaaa
     5821 cagcagataa gatcgttgtt ttctatgcgg gaacggctgt ggaagaagcg gataccgtgg
     5881 attttaaccg ggaggcggct ttgcgccatc cgtacaccag ggcgctgttt cgggcaatgc
     5941 cggagcatgg atttgccccg gaaccgggga tccagcctta tgtaagagat cttcctgagg
     6001 gttgtcccta tggccccaga tgccccaaat ataagaccga atgcagcaaa gaagtttcct
     6061 atgtgcccta tcagggggga ctggtgcgct gtatctgtcc gggagatgaa aatgagatct
     6121 tacctggaat attatcggga ccagcgggat taaagggaca aatgacgtca gagcaaataa
     6181 cgtcagagca gatggcgtcg ggacaaaggt ccggtgaata caatgcatgg ggaaaggaag
     6241 gcgtcagctt atgagattag aagcaaaaga cctgtctttc cgctatcata aaggaaacag
     6301 acagatatta aaccatgtat ctgtaagcct tgacagcagt gaccggttgg ggcttgcggc
     6361 acccagtggt tttggcaaga ctacgttctg taaaatactg gccggttatg aacagccaga
     6421 tgaaggccag gttttactgg acggaaagcc actttcggaa tataaaggtt actgtccggt
     6481 acagctgatc tggcagcatc cacaggaggc agtcaatccc agacgaaaga tgaaaacagt
     6541 tctggaagaa ggcggcgtat tagatgaacg cttgatccat ggtcttggga tcgagaagga
     6601 atggatggag cggtatcctg gggaactttc cggaggagaa cttcaaaggt tctgtattgc
     6661 aagggcatta ggtgaaaaaa ccagtttttt gctggcagat gagatcacag ccatgctgga
     6721 tctgatcaca cagagccaga tatggaattt tctgctggag gaagtacgga gccgggagat
     6781 cggactttta gtggtaagcc attcagagcc gctgcttcag cgaatctgta caggacggat
     6841 ccagttgtag agtagttgca ggacctatat taaaatcctg ctgtcggcag atattaaaaa
     6901 gtatacagat ccggatatcg acagagtgtt ttgccggtgc cggtggattt ctaatagaat
     6961 aattgtggat aaaaggagga gcatgaagca tggatgtgca taagatgctg gaacaggtaa
     7021 aagcgggcca gctggatatt gcagaggcag aggcgaagct taaagatctg ccttatgagg
     7081 atctgggcta tgcaaagctg gaccatcaca ggaaactgcg ttccggtttt ggcgaaacta
     7141 ttttctgcca gggaaaaccg gatccgtact taaaagagat attcctgaaa ttttatgaaa
     7201 gagacgggga agttcttgga accagagcca gtcaggccca gtatgagctg atccgttctc
     7261 tggtaccggc agcagtctat gatcctattt cccgcattgt aaaggtggaa aagccggata
     7321 aggagcttgt aggctgtgtg gcagtatgta ccggcggcac agcagatatc ccggtggcag
     7381 aggaggcagc ccagacagcg gagtatttcg gaacaaaggt agaccgcatc tatgatgtgg
     7441 gagttgctgg gatccacagg ctgttaagcc agagagagcg tatttgcaag gcaaactgca
     7501 ttattgcggt agcaggaatg gaaggggctc ttggaacggt gatcgcaggt ctggcagacg
     7561 caccggtgat cgcagtacct acatctgtag gatatggagc cagtttccat ggtctgtcgg
     7621 ctcttctgac catgatcaat tcctgcgcaa atggaatttc ggtagtaaat attgataacg
     7681 gctatggagc cggatatatt gcaacacaaa taaacagaat ggcggtgaag aaataatgaa
     7741 aaaaacatta tatctggagt gtaattcagg aataagcggt gatatgaccg taggagcgct
     7801 tcttgatctg ggagcagaca gaaaggtact ggaagatgca ctggcaagtc tgggagtaga
     7861 cggctatcat ctgcattttg gaacgacgga gaaatgcggc atcaaggcat atgattttga
     7921 tgtgcatctg gaatatgaag agcatgtaca tgagcattgc catgaccatg atcacgagca
     7981 tactcacgat catggagatc atgaccacga tcatggagat catgaccacg atcacgagca
     8041 tactcatgac cacggccata cccacgatca catgcaccca cacacccacc gcaacctcta
     8101 cgacatttac accattattg accgcctgca gtcttccgat aaagtaaaac agctggcaag
     8161 acgtattttt gaaattgtgg cagaggctga gtcaaaggca catggccttc caattgatca
     8221 ggtacatttc catgaagtag gcgccatcga ctctatcgtt gatattatca gtactgcagt
     8281 ctgcctggat aacttaggca ttgagaaagt cattgtttcc ccacttgcag aaggtcaggg
     8341 ctatgtaaga tgccagcatg gcaccttgcc agttcctgta ccggctacag cgaatattgc
     8401 acaggcatat ggcctgaagc tgcgctttac agaaacaccg ggagaaatgg tcacacctac
     8461 cggcgcggcg atcgcagcag cactgagcac cggtgcttcc atgccaaaga actatcgcat
     8521 cgtaaagatc ggtattggtg ccggaaataa ggattttgac catgcaaaca tccttcgtgc
     8581 catgctcatt gaggaagagg aagaagaaaa aacagacggc ttagagctgt tagagaccaa
     8641 tttagatgac tgcataggag aagcactggg atataccatg aacctgctgt taaaggccgg
     8701 agcaaatgac gtatggtata caccaatcta tatgaagaag aaccgcccgg cttatatgct
     8761 ttctgtatta tgccgtgaaa gcaaccgtga ggttatggaa gacattatct ttacccatac
     8821 cacatccatt ggcattcgtc gtttcccggc agagcgcact gccttagacc gcagaaagat
     8881 ccaggtggaa acagcctatg gaacagcgga tgtaaaggtt tgcagctata agggaaagaa
     8941 ttactgctat ccggaatatg acagtgtttc tgcattatgt gataaacagg aaaaggctga
     9001 ttttaacacg gtttaccagg cagcagcaaa agcggcgaaa gagcagttat aaagttatca
     9061 accaacagta tcagatgaaa acgctacatg caaatgaaaa ctgctaacag gagatacaaa
     9121 caggggcgca gcaaaaacag ataaccgggg aagacaaaca ttcattacaa gacatacagt
     9181 aaaggagacc ggaacgatgg aaggacagaa ttattcagta aaagaaagac tggaacagtt
     9241 agaagcactg atgggaaagt acacagctgg cggcaaagac ctttgcgtgg ctttttccgg
     9301 cggtgtggac agcagcctga tcttaaaggc agcagtaaag gcagctacga aggatggcag
     9361 caaggtttat gcagttacct ttgacagccg actgcatcca tcctgtgatc tggagattgc
     9421 aaaccgcgtg gcagcagaac tgggcggtgt ccacaaagtg atccatgtaa atgaactgga
     9481 acaggaagcc atccgaatga acccggtgaa ccggtgttat ctgtgcaaaa agcatctgtt
     9541 tgtaaattta aaggaaatgg caaaaagcct tggtgcagag actgttatgg atggaaccaa
     9601 tgaagatgac caccatgtat accgcccggg catccaggca ttaaaagagc tgggaatcgt
     9661 cagtccattg gcagaactgc acattaccaa ggcagaggta aaggccatgg caaaagagtg
     9721 gggcatttcc gtagcatccc gcccttcaac cccatgtatg gcaaccagac tgccgtataa
     9781 cactcccatc gactatgatg taatgaagca gatcgaagta ggcgaaggtt atttaagaga
     9841 gctgttaggc ggaaatgtgc gcctgcgtct tcatggaaat gtagtgcgta ttgaagcaga
     9901 taagacttca tttggaaaac tgttagagca tggagatgag attgtaaaga cattaaagaa
     9961 tcttggtttt gtttatatta ccatggatct ggaaggcttc cgttccggta gtatggatgt
    10021 tggcctggat aaataacaga agcggacaag caggcatgtc ggctgcatat ggtcatatga
    10081 atgcatgaac agtagaagaa tagtaaaaag ctcccaaccc aatgattttg ggcggggagc
    10141 tttttgttgc tttgcttagt gtgtagcacc ctcagcagct acaatatcgc tctcattttc
    10201 accaatagca gcgatggcag cctcaatacc tcttgcaata tcctgtaaat tcatggatgg
    10261 tgcagccggg cggtttacga cctggcttgg gataaatgga acgtgcatga agccgccacg
    10321 gactcccgga tagttcttag ccagagtgta gagaacacca tacatcaggt ggttgcatac
    10381 aaaggttcct gcagtgttgg aaacgctggc aggaagtccg gcatttcgca tattctgtac
    10441 catagcctta attggaagag tagcaaaata agcaggagca ccgtcttcaa agatcgtcac
    10501 atcaataggc tgattgcctt cgttgtcttt gatgcgggcg tcatctaagt tgatggcaac
    10561 acgctccggt gtcaggtcaa agcggcctcc ggcctgtcct atacagagaa cagcgtccgg
    10621 tctttctgcc tcaatagctt ttgctacttt atcaatggat ttgccgaaaa cagtaggtac
    10681 ttccagtttt actacctgga cagatccaac ggtatcggaa accagtttta cagcctctaa
    10741 agcagggttg acaggctctc cgccaaatgg atcaaatgca gtgagaagaa gtttcatagt
    10801 atatcatcct ttctgaaaaa ttgttgaaaa ttatttccca aaatatccca aaatattttg
    10861 agatttaccc ggtaaacttc gttaaatatt agatatagtt tatgcattgt atacaaaaaa
    10921 gaacaatatt ttaacgaaat gccagagtat acattaatac aatgtgaaca gcgagcatta
    10981 ccagtgcaac cggcagctgg cttaagataa caatgtattt gttctttgtt tcaagaagag
    11041 ctgcaggcag aatgttaaag ttagctgcca taggagtcat taaagtaccg cagtagccag
    11101 cagtcaggcc gagagcccct acaaccaccg ggtcagcgcc ctgtgcgatg aggaatggga
    11161 taccgatacc tacagtgata acagagaaag cggcaaaacc attacccatg atcatggtaa
    11221 ataaagccat tgcgatacag taaacggcag tagctgcaaa cagatttccg gccgggataa
    11281 tggcacttac gccccctgcg atcacatcgc caacaccggc agctgtaaat acagagccaa
    11341 gtgctgctaa aacctgagga aggataccgg taacacctac attgtcagta agacgggtac
    11401 agtcttttac cgcataaaca gcctttggcc tgaaaaagat aaaagcagtg accagggcga
    11461 tggagccgga gataccgata gcattattgg caccaagtac ctttaagaaa gtagcaacta
    11521 aaacagcgct gacagccagt accagcggtg ggataaatac gctgtagccc agtctgtcag
    11581 cagcagcacg ggtttctgcc tcttttggca tatcgctttt gcttggggct acacgtttaa
    11641 atgcagtaag gatggctaaa aatacaacgc acagacctgt gatccatttt ggaagataag
    11701 gtcctgcaat aaaggtaaat gcaaggatga accagaaaag agcagttgta gttctgtgag
    11761 cacagctttc atcttttaaa gccttcatac ccgttgcgat aaagataagt ccgatgatgc
    11821 agtagaaaac ttccgcaaca atggtgctta tgttccaggt catattattt atcctccttt
    11881 gcagctttgg atgccagacg gcgttttaag atctggtcaa agatcagggc gtagatcacg
    11941 cctactacag cagaaatcac ggcgatcgga acagactggc ctgcgatctg gagtgcgtct
    12001 acaggatagc cctgttcgct taaggtggat acgatcagaa gtgttccgga agatcccatg
    12061 aaacagttct gtgcaaagaa gttgccatag ttttcgcttc cggcgcacat tcccttgatc
    12121 tggtcttcgg atttctcatc cagttcctta tactgtacaa ctgctgccgc ctgggccata
    12181 ggattgatca gcgggcggat aaactgagga tggccgccga tacgcaggga gaaagcagag
    12241 gcaaaggtac ggatcacctg ccagatggcg atcagctttc ctgtggtcat gttctttaaa
    12301 gaacggataa agtccactgc cttatctttt aagccatttc gctcacacag tccgatcacc
    12361 ggcagtgtaa ggatgaaaag ggtggcagta cggttggtga taaatgcggt gcccagtgtg
    12421 ttcaggatat ccataggact catgcctgca accagtccgg tggcaagacc tgccacaacg
    12481 actacagcca gagtatctaa tttaaggata aagccgatca cgactataag tacaccaatt
    12541 aatttgatga tttccataaa taaaaaccct cctagttaaa atatagtatg gagaattgta
    12601 acataattat aatctgcggg caaggagaaa ggcctggaaa tcggggatag agcggatatt
    12661 gggaaaaatt gtcaaaaaga aaacgaagag tttgtgaaaa acgtaacgtc cgttagagaa
    12721 ttgttaagac aaaacaaaca aaaaaagtta gaatgtctaa aaaaaattgc aaacaatgca
    12781 aaaaatgcca ttgcttattt atagaatatc gagtataatc tattcaagtt gtacaaaatt
    12841 caaggggata atttcggtat ttcacgattg tatttataaa aaaacaacaa aaaacaccca
    12901 ggtgtttgat atactattga caaggaaagg aagatggtcc atgaaagact ggcagtatat
    12961 tttgaaaaat ctttcactac tcagccagat ggggatctcc ttagcggctc cgctgctgct
    13021 gtgtcttttt ttatgcagct ggcttaacag ccgttttatg ataggcgggt ggatctacat
    13081 ccctggtttt atcatgggac ttgggggttc ctttatgacg gcatataaat tatatcttgc
    13141 cattatgaaa acacagaaga agaaaaaaaa cagagaacat gagaaagcct ttaacaggca
//
LOCUS       DBMDUA010000068        13018 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2517_length_13018_cov_2.924054, whole genome shotgun sequence.
ACCESSION   DBMDUA010000068 DBMDUA010000000
VERSION     DBMDUA010000068.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 13018)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13018)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13018
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2517_length_13018_cov_2.924054"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="ACZPCL_06885"
     CDS             complement(<1..805)
                     /locus_tag="ACZPCL_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HIR1162225.1"
                     /translation="MFLKQRKHRLQNASIRSIIFMYFTITALAASVFIGVSLYGRLSG
                     QMSSALQEENHILINQLGRTMDSYLRLVMKLSDSLYYGVIKNADLSDGSINGEITLLY
                     DNNKDNVENIALFSKEGELLESVPAARLKNNAQVADQDWFTAALEKTENLHFSMPHVQ
                     HIFDTGDSQYRWVVSVARAVEITDGPYTDQGVLLIDLRYNSLEQLFDGMNLAKGGYVY
                     LTDSNGDLIYHPNGQLISSGLAMENNDNTAILRDGNYQEQWQGQTRVVSI"
     gene            complement(805..2442)
                     /locus_tag="ACZPCL_06890"
     CDS             complement(805..2442)
                     /locus_tag="ACZPCL_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1162226.1"
                     /translation="MKLYRIILVDDEEEVRKSIIRKIKWEENGFLVAGDAENGEDALE
                     KIEALEPDLILTDIRMPYMDGLTLAERVRQKYPSMKIVIFSGYDDFEYAKQAIKLNVT
                     EYILKPVNVEELTAILQKIKANLDQEIEQKRNVNLLRENYIKSLPILREQFLNELVNA
                     PVASETVETRLKEYGIPLSGAQKWIASAVGIDRPEEEGEGELAIHREQDLIPISVMQI
                     LEEKLGKYCRFSTFNSTGSSDAQIGVIAVIDEENTQTGLTAVLSDICKETRKILAVPI
                     TIGIGHSTQDLGMICESYKDAVNALGYKAIVGAGNTIYINDVEPVSRGKLQFDSKDEA
                     ELVGAVKFGPQEKIEEVVHKIADKISDAKVHQRQCQTYMLSVLNCMIQLVQMYDLDME
                     TIFQGESDLNEAFVLISSTQKKEDFVKWLLQAATAVNQSVNTERDNTTKQVIQEARQY
                     IMDNYQDADLSVEKICRHLHMSPAYFSTMFKKETGKTYVAYLTEVRLDKAVELLNKTD
                     DKTYVIAAKVGYQEQNYFSYVFKKRFGISPTKFRGAK"
     gene            complement(2459..3121)
                     /locus_tag="ACZPCL_06895"
     CDS             complement(2459..3121)
                     /locus_tag="ACZPCL_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014612.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HIR1162227.1"
                     /translation="MGLEEKVHIEQVLPADIEHRSFEIIGEELKAMGKELEPELAPVI
                     MRAIHTTADFDYADHLKFSENVVEKAREAIKKGAVIITDTKMGWSGVNKKRLESYGGE
                     ALCFMADEDVAAEAKKNGSTRAVASMDKAANLFGDGTRPCIFAIGNAPTALIRLYELI
                     QERKIKPALIIGAPVGFVNVIQSKELILSLKDTPYIVAEGRKGGSNVAAAICNALLYG
                     IK"
     gene            complement(3104..4633)
                     /locus_tag="ACZPCL_06900"
                     /pseudo
     CDS             complement(3104..4633)
                     /locus_tag="ACZPCL_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
     gene            complement(4638..5882)
                     /locus_tag="ACZPCL_06905"
     CDS             complement(4638..5882)
                     /locus_tag="ACZPCL_06905"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007036179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1162228.1"
                     /translation="MHTKYQHGGDIYSNKVSIDYSANINPLGLPQGVKEELARCIEEP
                     VCCVYPDSQCRDLVKALADYHKVTQDWILCGDGAADLIFGLAFALKPKKALLLAPSFL
                     EYEQALKAVDCDIDLFYLKEENGYRLDVEELCKTLERANATYNTTAVGCSDQQKSTSE
                     ANNGYNILFLCNPNNPTGIAVKKEQVLKLAETCEKTNTFLVVDECFEEFLDEPEAYSI
                     IPFLEKLSHVFVLKAFTKIYAMAGLRLGYALCSNEDVLTKICQVRQPWSVSGLAQRAG
                     IAALKETEYVLKTRKLIHEQREKMEAALTELGYIVYPSQANYIFFMEKTLEGATDSLY
                     DRMLKQGVLIRSCSNYPGLDASCYRICVKTEEENREFLHKLSQCTVTKRTDIKCADTK
                     HTDTVYTDANPTQAPHRPEKEE"
     gene            complement(6001..6975)
                     /gene="cbiB"
                     /locus_tag="ACZPCL_06910"
     CDS             complement(6001..6975)
                     /gene="cbiB"
                     /locus_tag="ACZPCL_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HIR1162229.1"
                     /translation="MEWIQLWQLHGLALLTGSILDWIFGDPYHFPHMIRLMGNMISGL
                     EKRLRKLFLEKPRFAGICLTLIMCLFWIIMPGLLFHITGRYLGLTARFILESFFCYQL
                     LAAKSLCVESMKVCKELKKGDTEGARKAVSMIVGRDTSVLDTAGITRAAVETVAENTS
                     DGVIAPFLFMAIFGPVGGTFYKAVNTMDSMIGYTNDKYILFGRTAAKLDDVLNFIPAR
                     ISGCLLTLAAYLLPGADGKNAWRIFLRDRRKHASPNSAHGEAACAGALHLRLAGDAWY
                     FGVLHKKQFIGDNDREIVPEDIWKASLLMFLAEGILMVVLLMILAAVR"
     gene            complement(7097..7615)
                     /locus_tag="ACZPCL_06915"
     CDS             complement(7097..7615)
                     /locus_tag="ACZPCL_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR1162230.1"
                     /translation="MIMVIGGRCQGKSSFAKEHFENRVQEKGKTQWEQEKEKTQEDHQ
                     KDPKADHWADGETSTWEEFLTSTWCRNFHLLVRRILKKDETLGLPDEQETALFETTSA
                     GLHNWKNMAETIYNANPDRILVTDEIGYGIVPIDPFEREYREETGRICCLLAEKSEEV
                     WRVCCGIGTRLK"
     gene            complement(7689..8474)
                     /locus_tag="ACZPCL_06920"
     CDS             complement(7689..8474)
                     /locus_tag="ACZPCL_06920"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HIR1162231.1"
                     /translation="MKWLESCLIAISMYSKIPVPQVEWTKEKMSHAMCFFPLVGVIEG
                     CLLGLWLFIAAQFNLSVGIRFLWASAIPFLVTGGIHMDGFMDTMDAVHSYGDRTKKLE
                     ILKDPHLGAFAVICAVVYLLLYTGSMYEFCENARGKAMFYPVLFLFSERVFSGLSVVM
                     FPSAKNNGLAAAFSQASEKKLDKKILLLSLVILAVLAFGIWDVQGLEVYGVCLVLQGV
                     LFAWYDKWSKKNFGGITGDLAGFFLQAEELICLTVVAFLLRIM"
     gene            complement(8477..9037)
                     /locus_tag="ACZPCL_06925"
     CDS             complement(8477..9037)
                     /locus_tag="ACZPCL_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR1162232.1"
                     /translation="MILITGGSGSGKSAYGEKRILEAGEMTRYYIATMEVFGEEGRKK
                     VERHKMLRQGKGFITIESPKDVGRVEKLEKTGNGKLTVLLECVSNLTANEMFGSSFGA
                     ELEPGRRTEALAEKICADIAKIDEAADFFAVITNEVGSDGEMYEKETLEYIRLLGLVN
                     CRLAKMASEVVEVVYGIPVMKKGAEV"
     gene            complement(9040..10140)
                     /gene="cobT"
                     /locus_tag="ACZPCL_06930"
     CDS             complement(9040..10140)
                     /gene="cobT"
                     /locus_tag="ACZPCL_06930"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009249149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HIR1162233.1"
                     /translation="MNQKQTVSLETVLKGIKPIDKEAVKSAKAHWDRVAKPLNSLGVL
                     EEDICRIAGVKRREEFSLDKKCIVIMCADNGIVAEGVTQTGQDVTAAVAKNMAARRSC
                     VCLMAACAGAEVLPVDVGIAADMTETGILDRKIAWGTRDFLKEPAMTRKEAVKALEVG
                     IEVAGILYEKGCDLAGTGEMGIGNTTTSSAVISVLTGLDPEVVTGRGAGLDHAGLKRK
                     IEVIKEGIRIHRPDPSDGIDVLSKVGGLDLAALTGFYLGCAYWRIPVVLDGLITGAAA
                     LAAVKLCPAVADYLLASHVSAEPAGQAVLASLGLKAAIQAGMCLGEGTGAAAFFPMLD
                     MALTIYSKMDTFQDIQVKPYCHFEEDKNEDKE"
     gene            complement(10142..11629)
                     /locus_tag="ACZPCL_06935"
     CDS             complement(10142..11629)
                     /locus_tag="ACZPCL_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HIR1162234.1"
                     /translation="MAGFMIAAPRSGSGKTMVTCGILELLKRKQKNPFSYKCGPDYID
                     GLFHRKVLGIEGGNLDSFFEQPQDIREKYAAVSETHFPVVEGVMGYFDGLGGDTTAAS
                     SWEVADILNLPVVLVVDAKGASLSLAALIKGFQVFQGESQSSQANKNGNHIQAVILNR
                     ISPMLYPRMKAVIEKETGVLVAGYVPELDFWQVGSRHLGLVLPGEIENLQEQMRQLGD
                     CLEKTLDVDVLLKLGEKADIDVLGSVTVTADGSSQKCEAQKNADLVLQNQFAGEKISH
                     SAIPIRLGVAWDEAFCFYYRDNLEFLEKQGVQIVQFSPVHDKHLPDDLDGLLLGGGYP
                     ELYAKALEENETIRNEIRRKAEEDMPILAECGGYLYLLKELEDENGTGYEMAGVFSGK
                     GYKKGKNSHFGYITVQTEKDSLYLKAGETIKGHEFHYWESTQDENELKMQAKKPTGKR
                     SWPCVTVKNQVMAGFPHLYYPSLEGFGERFVQACDRYRMKRKRQQ"
     gene            complement(11614..12930)
                     /gene="cbiE"
                     /locus_tag="ACZPCL_06940"
     CDS             complement(11614..12930)
                     /gene="cbiE"
                     /locus_tag="ACZPCL_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HIR1162235.1"
                     /translation="MSQNEYEERKPIIYLAGIGMGQDDQLTGEVLDCLDRADAVMGAR
                     RMLDAAAPYTEGKAVLAAYKPSEMVNWLSSFEWDEAVLVLSGDTGFYSGAEAASKAFL
                     QEGWEVEYIPGVSSLSYFCARLGRSWQNVHSISSHGRSCDVVSHIRHYEDCFILLGGK
                     GSVSALCRELVSNGLGNVKIWAGENFSYETERILCEATPAELLMEEEKRPFGSLACVI
                     VKNPHAEEMRIYLTKHPKDEDFIRGKVPMTKETIRRLSIEKLYIGNHSVCYDIGAGTG
                     SVSVEMGLAIRRSCNEGSVYAIEREPEALELIRANVQKFHGNWEDIHIVEGEAPEAFE
                     GLPAPTHAFIGGSGGKMKEIIGTLLNLNPAVRIVANAITLETVSEILACMKEYGFEDG
                     ELLQVQVSPVKQLGSYHMPIAQNPVYIAVMQHPVKDDVEEIEWLDS"
BASE COUNT         3138 a         3462 c         2556 g         3862 t
ORIGIN      
        1 ttatagatac tacccgggtc tgtccctgcc attgctcctg ataatttcca tcccttaaaa
       61 tggctgtgtt atcattattt tccattgcaa ggccggagct gatcagctgg ccgttcgggt
      121 gatagatcag gtccccgttg ctgtctgtaa gataaacata gcctcctttt gccaggttca
      181 ttccgtcaaa cagctgttcc agactgttat agcgcagatc aatgagcaaa acgccctgat
      241 ctgtataagg accgtctgtg atctctactg ctcttgccac agataccacc caacgatact
      301 ggctgtctcc tgtatcaaaa atatgctgta catgaggcat ggaaaaatga agattttctg
      361 ttttttccaa tgccgccgta aaccagtcct gatctgccac ctgggcattg ttttttaatc
      421 ttgctgcagg aacagactca agcagttctc cttctttgga aaacagggcg atattttcta
      481 cattatcttt attattatca taaagaaggg tgatctcccc gttaatagaa ccatctgaaa
      541 gatcggcatt tttgatcacg ccgtaataca gggagtctga aagtttcatt accagacgca
      601 ggtaggagtc catggtccgg cctaactgat tgataagaat gtgattttcc tcctgaaggg
      661 cagaggacat ctgccctgac agccgtccgt aaagggaaac tccgataaaa acgctggctg
      721 ccagagcagt aatggtgaaa tacataaaga taatagaacg gatactggca ttttgaagcc
      781 ggtgttttcg ctgtttcaaa aacattattt tgctcctctg aattttgtag gtgagatacc
      841 aaaacgtttc ttaaacacgt agctgaaata attctgctcc tgatatccta cttttgccgc
      901 gatcacatag gtcttgtcat ccgttttatt taaaagttcc acagctttgt ctaaccgcac
      961 ttccgtcaga taagccacat aggtctttcc tgtttctttc ttaaacatgg tggagaaata
     1021 agccgggctc atatgcaggt gacggcagat cttctccaca gacagatccg catcctgata
     1081 attgtccata atatactgtc tggcttcctg gatcacctgt tttgtggtat tatcacgctc
     1141 tgtatttaca gactggttta ctgctgtggc cgcctggaga agccatttta caaaatcttc
     1201 ctttttctgg gtgctggaga tcaggacaaa ggcctcattt aagtcagact ctccctggaa
     1261 aatggtctcc atatccagat catacatctg caccagctgg atcatacagt ttaacacact
     1321 taacatatag gtctggcact gacgctgatg gacttttgca tcactgatct tgtcggcaat
     1381 tttatgtact acttcctcga tcttttcctg agggccgaat tttacagcac ctaccagttc
     1441 tgcctcgtct ttgctgtcaa actgcagctt tcctctgctt accggctcta catcattaat
     1501 gtaaatggta tttccggctc ctacgattgc cttatagccc aaggcattta ccgcatcttt
     1561 ataagactca cagatcatgc ccaagtcctg ggtgctgtga ccgataccaa tagtgatggg
     1621 cactgcaagg atctttctgg tttctttgca gatatcactt aatacagcag tcagaccggt
     1681 ctgggtattt tcctcatcaa tcactgcgat cacaccgatc tgggcatcgc tgctgccagt
     1741 ggaattaaaa gttgaaaaac ggcagtattt tcccagcttt tcctctaaga tctgcataac
     1801 agaaataggg atcaggtcct gttcacggtg gatcgccagt tccccttcgc cctcttcttc
     1861 cggccggtca atgcctaccg ctgatgcgat ccatttctgt gcacctgaaa gaggaatgcc
     1921 gtattctttt aaacgtgttt caacggtctc agaagcaacc ggggcattta ccagctcatt
     1981 taaaaactgt tcacgaagga tcggcaggct tttaatataa ttttcccgca gcaggtttac
     2041 atttcgcttc tgctcgatct cctggtccag atttgccttg atcttctgta aaatggcagt
     2101 cagctcttct acgtttaccg gctttaatat gtactcagtt acatttaatt taatagcctg
     2161 cttcgcatac tcaaagtcat cgtaaccaga gaaaataacg atcttcatag acgggtattt
     2221 ctggcgcaca cgctctgcca gtgtcagtcc gtccatataa ggcatgcgaa tgtcggttaa
     2281 gatcaggtca ggctctaaag cttcaatctt ttctaaggca tcttccccgt tttccgcatc
     2341 ccctgctact aaaaatccat tttcctccca tttgatcttg cggataatgc tctttcttac
     2401 ctcttcctca tcatctacaa ggataatacg atatagtttc atgtattttg tccccttttt
     2461 attttatccc atatagcaaa gcgttgcaga ttgccgctgc tacgttgctt ccaccttttc
     2521 ttccttctgc cacgatgtaa ggcgtgtctt tcaatgaaag gatcagttct ttggactgga
     2581 tcacattcac aaagcctacc ggcgcaccga tgatcagggc cggttttatt tttctctcct
     2641 ggatcagctc atacaggcgg atcagagccg ttggggcatt tccaatggca aagatacagg
     2701 gtcttgttcc gtctccaaac agattggctg ccttatccat acttgcaacc gctcttgtgc
     2761 tgccgttttt ctttgcctct gctgccacat cttcatctgc cataaagcat aaagcttctc
     2821 ctccgtagct ttccagccgc tttttattta cgccggacca gcccattttc gtatcagtaa
     2881 tgatcactgc tcccttttta atggcttctc ttgccttttc tacaacattt tcggaaaatt
     2941 tcagatgatc tgcataatca aaatccgctg ttgtgtggat agcacgcatg atcaccggtg
     3001 ccagttccgg ttccagttcc tttcccatag cctttaattc ttccccgatg atctcaaagc
     3061 ttctgtgttc tatgtctgcc ggaagcacct gttctatatg caccttctct tccagcccca
     3121 tgatctcata tatcttttcc atatccagac tctcccgcag tatttctgcc agtttatcat
     3181 actgttcttc cttatatgct atgtaatcca tctgccctgc catttccagg gtaatgccct
     3241 ttctggctgc cagggcagtt gctatggtct cgcagatgcc gggggcatca aatacgccat
     3301 ggacatagga accatacagg tttttccggt tccagccaca ggggataccc ctggcttctc
     3361 tgccatttgc actttctcct gcatttaaca ggcacagtgg ctgttctttt tcccggctct
     3421 ttgtacggcc catatggatc tcataaccag ttaatctctt tccggaaagt tccctgaaac
     3481 agccgtaaag ttccccgaat cggccttctg tctgggtgcg tactttttct tcttcaaatt
     3541 cagtttctac aggaaaaaga cccattccac ggatctttcc tttgtgggat gactcaatgg
     3601 caagttcgtc caccagccat tctcccatca tctggaaacc gccgcaaatt ccccatacag
     3661 gaacctggcg ggctgccagc ttaaggatcg ctgcttccag gccgatctgg cgcatccaca
     3721 ataaatcatc aatggtattt ttcgttcccg gaaggatcac catgtctggc gtttttaatt
     3781 cagaaggctt tgagacataa cggatggaga cctcatccat acgctcaaat acatccatat
     3841 ctgtaaagtt ggaaattttt ggaaaacgga tcaccgcaat gtcgatgcag cccttctgtc
     3901 ttgtatttcc cagtttgctg cttaagctgt cctcatcttc gatatctaca ttcatatagg
     3961 ggacaacgcc tgccaccggt atcttacaaa gatcttctaa ctggcgaagt cccggctcca
     4021 ggatcgactt atctccccgg aatttattaa taataagccc ttttatgcgg ttccgctctt
     4081 caggctctaa aagttctact gtgccgtaaa gctgggcgaa cacaccgccc cggtcaatat
     4141 ccccggcaag gagcaccggc gcatctacca tggcagccag tcccatgttt acaatgtcat
     4201 cggattttaa gttgatctct gccggacttc ctgcgccttc aatgacgatc acgtcatagg
     4261 cttcgtccag acgtttgtat gcttccatga tctgtgggat cagctgcttt ttgtaggcaa
     4321 aatagtcttt tgcccgcata tttccgatgg aaacaccgtt tacgatcacc tgggaaccca
     4381 catcggtagt gggtttgagc aaaatagggt tcatatccgc agaaggcttt actcctgccg
     4441 cctctgcctg gaccacctgt gcccggccca tttccaggcc gtcttctgta ataaaggagt
     4501 taagagccat gttctgggat ttaaaaggcg ctgttttata gccgtcctgg gcgaaaatgc
     4561 ggcacaggcc cgctgcgatc agactttttc ctgcattgga catggttccc tgtatcataa
     4621 tggatcttgc catggtttta ttcctccttt tcaggtctgt ggggtgcttg tgttggattg
     4681 gcatccgtat atacggtatc tgtatgtttt gtatctgcac attttatatc tgtacgtttg
     4741 gtgactgtac attgggaaag tttgtgtaag aactctctgt tttcttcttc tgtttttacg
     4801 cagatgcggt agcaggatgc gtccaggccc ggataattgg agcagctgcg gatgaggacg
     4861 ccctgtttta gcatgcggtc gtacaggctg tctgttgctc cttccagagt cttttccata
     4921 aagaaaatat aattggcctg ggacggatag acgatatagc ccagttctgt taatgccgcc
     4981 tccatttttt ctctctgctc atggatcagt tttctggttt ttaatacata ttccgtctct
     5041 tttaaggctg caatgcctgc tctctgggcc agtcctgaaa cagaccaggg ctgtcttacc
     5101 tgacagatct ttgttaaaac gtcttcattg ctacacagag cataacctaa acgcaggcct
     5161 gccatggcat agatcttggt aaatgccttt aaaacaaaga catgggagag cttctctaaa
     5221 aacggaataa tgctgtaagc ttccggttca tctaaaaact cttcaaaaca ctcatccaca
     5281 accagaaagg tatttgtttt ttcgcaggtt tcagccagtt ttaagacctg ctcttttttt
     5341 acggcaatgc cggttgggtt gttggggttg cacaggaata aaatgttgta gccgttgttg
     5401 gcttctgatg tagatttctg ttggtcagaa caaccgactg cggtagtatt ataagtggca
     5461 ttagcccgtt ccagcgtttt acacagttcc tccacatcca gacgatagcc attttcctct
     5521 ttgagataaa aaagatcaat atcacagtcc actgccttca atgcctgctc atattccagg
     5581 aaagaaggag ctaaaagcag tgcttttttc ggctttaagg caaatgcaag tccaaagatc
     5641 aggtccgctg ccccatcacc gcagaggatc cagtcctgag tcaccttatg gtagtcagct
     5701 aatgccttta ccagatccct gcactggctg tccgggtaaa cgcagcagac tggctcttct
     5761 atacaacggg ccagctcttc ttttactccc tgaggcagtc ctaaaggatt gatattggcg
     5821 gaatagtcta tagaaacttt attgctgtaa atgtcaccgc cgtgctgata tttggtatgc
     5881 atatgaattc cttctttcca aaatgaatat ctatactctg ttcatctccc agcccggctg
     5941 gggatgatct tgaaccagtt atttgaatat ctgtatttgt atttccgtga ttatcaccta
     6001 tcacctgact gctgccagga tcattaacag caccaccatc agtatcccct ctgccaggaa
     6061 cattaaaaga gaggctttcc agatatcctc cgggacgatc tccctgtcat tatccccgat
     6121 aaactgcttt ttgtgaagaa ccccaaaata ccaggcatcc ccggcaagac gtaaatgaag
     6181 ggctccggca caggcggctt ctccgtgggc tgagttgggg ctggcatgtt tacgccggtc
     6241 tcttaaaaat atgcgccagg cattttttcc gtctgccccc ggaagcaggt atgctgccag
     6301 tgtcagcaga cagccggaaa tgcgggccgg gataaaattt aacacatcat ccagctttgc
     6361 agctgtccga ccaaataaaa tgtatttatc attggtatag cctatcatgg agtccatggt
     6421 attcactgcc ttgtaaaaag tgccgcctac cggtccgaaa atggccataa acaggaacgg
     6481 ggcgatcaca ccatctgagg tattttctgc cactgtctcc accgccgctc tggtgatacc
     6541 tgctgtgtcc agaacactgg tatcacggcc tacgatcatg gaaactgcct ttctggctcc
     6601 ttctgtatct ccctttttca gttctttgca taccttcata ctctccacac aaaggctttt
     6661 tgcagccaga agctgataac agaaaaagct ttccagaata aatcttgcag tgaggccaag
     6721 atatcttccc gttatatgaa agagcagacc aggcataatg atccagaaaa gacacatgat
     6781 cagggtaaga cagattcctg caaaccgtgg cttttcaaga aaaagttttc gcagtctctt
     6841 ctccaatccg cttatcatat ttcccatcag gcgtatcata tggggaaaat gatagggatc
     6901 cccaaagatc cagtccagaa tactgcctgt taaaagggcc agcccatgaa gctgccacaa
     6961 ttgtatccat tccatgttac tatcctttcg tttctcttat accatttggc gttattactg
     7021 ttcatgcatt gcgcgaccag caatggaatt tgccgtggtt tcacattgaa atacagcaaa
     7081 ttgcaccttt ttctccctac tttaaccgcg ttccaatccc acagcagacc cgccagactt
     7141 cctctgactt ttctgccagc agacagcaga tgcggcctgt ttcttccctg tattcccgtt
     7201 caaagggatc aattgggaca ataccgtaac caatctcatc tgtaactaaa atgcggtccg
     7261 ggttggcgtt ataaatggtt tctgccatat ttttccagtt gtgaagacct gcagatgttg
     7321 tttcaaataa agctgtttcc tgctcatccg gcaatcccaa tgtctcatct ttttttaaaa
     7381 tccggcgtac cagcaggtga aaattccggc accaggtgga agtaagaaat tcttcccagg
     7441 tggatgtttc cccatctgcc cagtgatctg cctttggatc tttctgatga tcttcctgcg
     7501 ttttttcttt ttcctgttcc cactgagttt ttcctttttc ctgtacccta ttttcaaaat
     7561 gttcttttgc aaaggaagac tttccctgac atcttcctcc aatgaccatg atcattcgat
     7621 ttttctcctt cggttctcct atgcttatga accctgtttt caacttattt ttacaagttc
     7681 agatattttc acattattct caataaaaat gccactaccg tcagacatat cagttcctct
     7741 gcctgcagga aaaatccagc caggtctccg gtaatacctc cgaaattctt tttggaccat
     7801 ttatcatacc aggcaaaaag cactccctga agcaccaggc agactccata aacttccagt
     7861 ccctgtacgt cccagatgcc aaaagccagc actgcaagga tcaccagtga caggagcaat
     7921 attttcttat ccagcttttt ctcagaagcc tgggaaaagg cagctgccag cccattattt
     7981 ttcgctgacg gaaacatgac cacggaaagg ccgctgaaca cccgctcact gaatagaaac
     8041 aataccgggt aaaacatagc ctttccccgg gcattttcac aaaattcata catgcttcct
     8101 gtatataata acagatatac aaccgcacag atcactgcaa aggcccctaa atggggatct
     8161 tttagtattt ccaacttttt cgttctgtct ccataggaat ggaccgcatc catagtgtcc
     8221 ataaaaccgt ccatatggat gccccctgtt acaagaaagg ggatggccga cgcccaaaga
     8281 aatcttatcc ccacagacag attaaactgc gctgcaataa agagccaaag ccctaaaagg
     8341 catccttcta taacacctac cagcggaaaa aagcacatgg catggctcat tttttccttg
     8401 gtccactcta cctggggtac aggaatcttt gaatacatgg aaattgcgat cagacaggac
     8461 tccaaccatt tcataattac acttctgctc cttttttcat aaccggtata ccgtatacta
     8521 cttcaactac ctctgaagcc attttcgcca gacggcagtt aacaagacct aaaaggcgga
     8581 tatactctaa ggtttctttt tcatacattt ccccatcaga gcctacttca ttggtgatca
     8641 ctgcgaaaaa atcagctgct tcgtcaattt tggcaatgtc agcgcagatc ttttccgcca
     8701 gcgcttctgt gcgtctgcca ggttctaatt ctgctccaaa agaactgcca aacatttcat
     8761 tggctgtgag attggaaaca cattccagca acactgtcag tttgccattt ccagtttttt
     8821 ccagtttttc cacccgtccc acatctttcg gactctcaat ggtaataaat ccttttccct
     8881 ggcgaagcat tttgtgccgc tctacctttt tcctgccttc ttccccgaat acttccatgg
     8941 tagcaatata ataccgggtc atttctcctg cttccagtat tcttttttct ccataggcag
     9001 atttaccgct gccgcttccc ccggtgatca gtatcatagc tattctttat cctcgttttt
     9061 atcttcttca aaatgacagt aaggctttac ctgtatatcc tgaaaggtat ccattttgct
     9121 gtaaatagta agggccatat caagcatagg gaaaaacgca gctgctcccg ttccttctcc
     9181 caggcacatt cccgcctgaa tggctgcttt taagccaaga ctggctaaaa ctgcctgtcc
     9241 tgccggttcc gcggacacat gggatgccag aagatagtca gccacagccg gacaaagctt
     9301 cactgccgcc agagctgctg ccccggtaat aaggccgtct aaaaccaccg ggatccgcca
     9361 gtaggcacag ccaagataaa aacctgtcag tgccgccaga tccaggccgc ctacttttga
     9421 taaaacatcg atcccgtcag aaggatccgg tctgtggata cggattccct ctttgatcac
     9481 ttctatttta cgcttaagac ctgcatgatc aagacctgcg cctctgcctg ttaccacctc
     9541 tggatccaga cctgtaagta cagagatcac agcgctggaa gtggtggtat ttccaatgcc
     9601 catttctcct gtaccagcca gatcacagcc tttttcatat aagataccgg ctacctcgat
     9661 ccccacttcc aatgctttta cagcctcttt tctggtcatg gcaggttctt ttaagaaatc
     9721 tctggttccc caggcgatct tacggtctaa aattccggtt tctgtcatat ccgccgcgat
     9781 ccccacatct acaggaagga cttctgcccc tgcacaggct gccatcaggc agacgcagga
     9841 tcttctggct gccatatttt tggcaactgc tgctgttacg tcctgtcctg tctgggtcac
     9901 accttctgct acgatcccat tgtccgcgca catgatcaca atacattttt tatccaggga
     9961 aaattcttcc cgccttttta cccctgcaat gcggcagata tcttcttcta acacgcctaa
    10021 actgtttaat ggctttgcca cccggtccca gtgggctttt gctgacttta ctgcttcttt
    10081 atcaatcggt tttatcccct ttaacacagt ttccagggaa acagtctgtt tctggttcat
    10141 tttactgctg cctctttctt ttcattctgt atctgtcaca ggcctgtaca aaccgttccc
    10201 caaaaccttc cagggaagga taatacaaat ggggaaaacc tgccattacc tgatttttta
    10261 cagtgacgca gggccagctg cgctttcctg ttggcttttt tgcctgcatt ttgagttcat
    10321 tttcatcctg ggtactttcc cagtaatgga attcatgacc tttgatggtc tcgcctgctt
    10381 ttaaatacag actgtccttt tctgtctgta ccgtaatata gccgaaatga gaattttttc
    10441 cttttttgta gcctttcccg gaaaatacac ctgccatctc atagccggtt ccattttcat
    10501 cttccagttc ttttaaaaga tagagataac cgccgcactc tgccagaatg ggcatatctt
    10561 cctctgcttt ccggcggatc tcatttcgta tggtctcatt ttcttccagc gcttttgcat
    10621 aaagctccgg ataaccgccg ccaagtaaaa gaccatccag atcatctgga agatgcttat
    10681 catggacagg gctgaactgg acgatctgaa cgccctgttt ttccaaaaat tccagattgt
    10741 cccgatagta gaagcagaaa gcttcatccc aggcaacgcc cagacggatg ggaatagctg
    10801 aatgtgagat tttttctcct gcaaattggt tttgcaaaac caaatcagca tttttctgag
    10861 cttcacactt ctggctggaa ccatctgctg tcacagtcac agaccctagc acatctatat
    10921 ctgctttttc tcccagctta agtaatacat ccacatccaa tgtcttctcc agacagtcac
    10981 ctaactgcct catctgctcc tgaaggttct cgatctcccc aggaagcaca agtcctaaat
    11041 gacggcttcc cacctgccag aaatccagtt ccggtacata tcctgctacc agcacacctg
    11101 tttctttttc gatcactgcc ttcatccgcg gatataacat aggagagatc ctgtttaata
    11161 tcactgcctg aatatgattt ccatttttat ttgcctgaga agactgggac tccccctgaa
    11221 agacctgaaa gcccttgatc agcgccgcca gggaaaggct ggctcctttt gcatccacta
    11281 ccagcaccac cggcagattt aaaatatccg ctacctccca ggaactggca gctgttgtat
    11341 ctccgcctaa gccgtcaaaa tatcccatta cgccttccac tactggaaaa tgcgtttcag
    11401 acactgctgc atatttttcc ctaatatcct gtggctgttc aaagaaactg tccaggtttc
    11461 ctccctcaat gccaaggact ttccgatgaa acagcccgtc aatatagtcc ggtccacatt
    11521 tataggaaaa tggatttttc tgttttcgtt ttaaaagttc caggatgccg caggtgacca
    11581 tagtctttcc gctgccgctt cttggagctg cgatcatgaa tccagccact ctatctcctc
    11641 cacatcgtct tttaccggat gctgcatcac tgcaatgtat accggattct gagctattgg
    11701 catatggtag ctgccaagct gtttaacagg agaaacctga acctgcagaa gttcaccgtc
    11761 ttcaaaccca tattccttca tacatgcaag gatctctgat acagtttcca aagtaatggc
    11821 atttgccacg atccgcaccg ccggatttaa gtttaacagg gtaccaatga tctccttcat
    11881 ctttccgccg ctgccgccga taaaagcatg ggtaggtgcc ggaagtccct caaaagcctc
    11941 cggtgcttct ccctctacaa tgtggatatc ttcccagttt ccatgaaact tttgcacatt
    12001 tgcccggatc agttccaaag cttccggttc ccgctcaatg gcatacacac tgccttcatt
    12061 acagcttctt cggatcgcca gacccatctc cacggataca gagcccgttc ccgcgccaat
    12121 atcataacag acactgtgat taccgatata aagtttctct atagacagcc ggcgaatggt
    12181 ttccttggtc atcggaacct ttccccggat gaaatcttcg tctttcgggt gctttgtgag
    12241 ataaattctc atctcttctg cgtgaggatt tttcacaata acgcaggcaa gacttccaaa
    12301 gggcctcttc tcctcttcca tcagcaattc tgcaggggta gcttcgcaga gaatacgttc
    12361 tgtttcgtat gagaaatttt ctccagccca tattttcaca tttccaagtc cattacttac
    12421 cagttccctg caaagagcag aaacactgcc ctttcctccc agtaagataa aacagtcttc
    12481 ataatgacgg atatgggaaa ctacgtcgca gctgcggcca tggctgctga tactgtgaac
    12541 attctgccag cttctgccga gtctggcgca gaaataagaa agactggata caccgggaat
    12601 atattctacc tcccagcctt cctgcaggaa agctttggaa gccgcttctg ccccgctgta
    12661 aaagccggta tccccggaga gcaccagcac tgcctcatcc cattcaaaag aagaaagcca
    12721 gtttaccatt tcactgggct tataagctgc cagcactgct tttccttctg tataaggtgc
    12781 agctgcatct aacatccttc tggctcccat tacagcatct gcccggtcta agcagtctaa
    12841 aacctcgcct gtcagctggt catcctgccc cattccgatc cctgcaagat agataatggg
    12901 ttttctctcc tcatattcat tctgagacat ccttcatcca ctcctttact gcttcttttg
    12961 tctgctctaa ggacattcct ttatcttttg gccggtcgat cacaacagct gttgctcc
//
LOCUS       DBMDUA010000069        12729 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2570_length_12729_cov_3.258948, whole genome shotgun sequence.
ACCESSION   DBMDUA010000069 DBMDUA010000000
VERSION     DBMDUA010000069.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 12729)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12729)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12729
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2570_length_12729_cov_3.258948"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2269
                     /locus_tag="ACZPCL_06945"
     CDS             <1..2269
                     /locus_tag="ACZPCL_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013180.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1162236.1"
                     /translation="IEIRGKTITETKEDDRKIVGFVRDIHETKMRELKQERKQMIDPV
                     TLFYRLKPGMEVIEKVRGKYPQGMFILVDICHFTIITRDFGLTFRDVVLEEFSKMFLE
                     VCAEDLSRPPVMIRAGSDELLAWIPKITEESCRQKLEKLKERFSVMIRRSALNLNFQA
                     GIARGDESVTTTSLLRRVRIAVTEAEKQEIGVLSWNEVKDPGAQPKTFGKIVSMGYME
                     QISLASLALNLLDRCPSIDAGLDLLSRRLKARYGMENMLITSFHYDFLSSELEYQWKH
                     LHGLMGKRGVVHCTEEEYEKLNQDAQINAVQPVKQLLSVVPVFRKTDIDQTGVGILMS
                     DNGQYSGSIFLLGIEPSVYENEEDRKLLWEIGIIIQNRINRDRHDQSAQAKSDFLAHM
                     SHEIRTPMNGIIGMTEIALKEGQTEEVRLDCLKKVKGSSKYLLGLLNDILDMSKIESG
                     KMKLVKDDFQLDKMIGELYPLLEAKFEEKSQKYHQEIHLVNQWFYGDALRLSQVLVNL
                     LSNAVKYSGDHTEITLTVEETRLSDGLSKLQFAVKDHGIGVSEEDRQRIFRSFEQVDR
                     SAVGQQGTGLGLAISNRLVHMMGGIIELESQIGEGSTFRFSITLKPVSAHHVKEEVSR
                     VERDFTGVRVLVTEDNPLNREILQYILEDMGMSVENACDGKEAVDKFTASEKGYYDLI
                     IMDIMMPVMNGLEAAHAIRTTDHPDHKKIPIIAISANAFDEDIRRSLASGMNAHLSKP
                     IEVDKLRETLGKLLK"
     gene            complement(2274..>2637)
                     /locus_tag="ACZPCL_06950"
     CDS             complement(2274..>2637)
                     /locus_tag="ACZPCL_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162237.1"
                     /translation="VVYKDGQEVERTLDHSKTYLGHSAVIHRNTSSASGTTTSGTTTS
                     DTTASSTKGAQESSSVANPTKAAAPTKAGTTEKKAPKHETAPAQTAPAPTEPAPTAPA
                     APETSQPAPEETIAPFVQ"
     assembly_gap    2638..2647
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2648..3990)
                     /locus_tag="ACZPCL_06955"
     CDS             complement(<2648..3990)
                     /locus_tag="ACZPCL_06955"
                     /inference="COORDINATES: protein motif:HMM:NF016202.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR1162238.1"
                     /translation="MSLNKNSRSSYHGPRGRRDKDHTPKRLNFDYDKPKRKPIKGGWI
                     PFAAAGAVVVLLGISAAVLHSRSVSEPEGAAKATASQASETTPVLLTVNGISLEGLSK
                     DEAVSRISKSLSWKMTAAYEDDSKELPNILTDAIRDWLTSKEEETVSGDLTLSAAELP
                     TDALKNSISQELEELAKEWDVPSKISTLDHYDNQKGQFVFREGCAGSRIDRDQLANDI
                     LAAIHENRMDAVVEVQSHKEAPSISCDEVKASYKTLVTFTTETTDNDKRNTNVRLAAE
                     ALNGTIVQPGEEFSFNSVVGQRTAEKGYQEAAAYNSGAVVQELGGGVCQISSTLYRVV
                     FQSGMEVTFRRSHTFEPNYVTPGQDAAISWEQPDFRFVNTSDKPIGILASYADRKATV
                     SIFGVPVLEDGVVWDLASEKVEEIDPPEPEYVEDPTLAPGTENTIKAATKGSRWDT"
     gene            complement(4120..6240)
                     /gene="feoB"
                     /locus_tag="ACZPCL_06960"
     CDS             complement(4120..6240)
                     /gene="feoB"
                     /locus_tag="ACZPCL_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR1162239.1"
                     /translation="MKQPKAIPDHYPVIALAGNPNVGKSTLFNSLTTGKKQHTGNWIG
                     KTTANAWGICHSPARDYTLVDIPGTYSLSSRSAEEEVARDFICFGAHQAVVIVCDATC
                     LERNLNLVLQILEATDRTLVCVNLLDEAEKKNITIDLDQLSDLLCVPVAGTSARKKST
                     LSSFLERLDDITEGPAIPHYQVTYCPELEKAVSMVEPAVKNWLDTHELPFYTTSRFLS
                     LRLLEDDPSFLRSFTNACQLVPSQDPAIADCLKTAWDHLNRKGIFKDILKDRIVSGLI
                     DNAETIASLCVKLPERSYNETDRKLDRILTGRLAAYPAMAGLLALIFWITLVGANDLS
                     ALLSRLFFSFQPVLDQALLSLHFPDWIRDAFVHGVYQVLAWVVSVMLPPMAIFFPLFT
                     LLEDSGYLPRIAYNLDRPFQCCKACGKQALTMAMGFGCNAAGITGCRIIDSPRERLIA
                     MITNSFVPCNGRLPLFITLILLFLSGNSHGLLSSAASALLLTLFILSGIAMTFITSYL
                     LSATILKGVSSSFTLELPPYRKPQIGKVIFRSVTDRTLFVLGRAAAVAAPAGFLLWIM
                     ANTAPGNISLLARFSRFLDPLGRLMGMDGVILTAFILGLPANEIVLPIILMAYTAQGS
                     LTNFENMGQLKEILLLHGWTWKTAVCVLLFSLMHWPCSTTLITLYKETKSKWWTFVSA
                     FLPAFCGALCCIIFTVLTRLFQTF"
     gene            complement(6237..6482)
                     /locus_tag="ACZPCL_06965"
     CDS             complement(6237..6482)
                     /locus_tag="ACZPCL_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1162240.1"
                     /translation="MTSPLCLNDIKPGEKARVCCLTSSGSIRRRFLDIGLVENTLVEC
                     VGISPLGDPAAYQIRGAVIAIRKADSRNIQICRKEKA"
     gene            6653..7144
                     /locus_tag="ACZPCL_06970"
     CDS             6653..7144
                     /locus_tag="ACZPCL_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6106 family protein"
                     /protein_id="HIR1162241.1"
                     /translation="MEENYIEWLVKRKDPVYAWPVKILMIVVCALSVLAALQMLLGIL
                     ILAAAAAATYFVFLNLSVEYEYLFADGGFSVDSILGKARRKKTFDCDKEDVQVIAPAN
                     SYVLKDYEKQGMKVIDCTSHNAGADVYALISQKGAQTTKVLFEPGDKMKAAMRRVFPR
                     KFI"
     gene            7352..8806
                     /gene="guaB"
                     /locus_tag="ACZPCL_06975"
     CDS             7352..8806
                     /gene="guaB"
                     /locus_tag="ACZPCL_06975"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR1162242.1"
                     /translation="MGTIIGEGITFDDVLLVPSYSEVIPNQVDLSTNLTKKIRLNIPL
                     MSAGMDTVTEHRMAIAMARQGGIGIIHKNMSIEAQAEEVDRVKRSENGVITDPFYLSP
                     EHTLKDANDLMAKFRISGVPITEGKKLVGIITNRDLKFEEDFSRPIRECMTSKNLVTA
                     KEGVTLKEAKAILAKARVEKLPIVDDDFNLKGLITIKDIEKQIKYPLSAKDAQGRLLC
                     GAAIGITANVLERVEALVKAKVDVVVLDSAHGHSANVIRCVKMIKEAYPDVQVIAGNV
                     ATGDATRALIEAGADCVKVGIGPGSICTTRVVAGIGVPQITAVMDCYAVAKEYGIPII
                     ADGGIKYSGDITKAIAAGGSVCMMGSMLAGCDESPGAFELYQGRKYKVYRGMGSIAAM
                     ENGSKDRYFQTDAKKLVPEGVEGRVAYKGMVEDTVFQLLGGLRSGMGYCGAKDIPTLQ
                     ETGRFVKISAASLKESHPHDIHITKEAPNYSIEE"
     gene            9046..9531
                     /locus_tag="ACZPCL_06980"
     CDS             9046..9531
                     /locus_tag="ACZPCL_06980"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1162243.1"
                     /translation="MGERSKLTTLCYIEKDDSYLMLHRVSKKNDENKDKWIGIGGHFE
                     EGESPEDCLLREAKEETGLTLTSWKFRGIVTFVSDVWGTEYMCLYTADGYTGEMISCN
                     EGVLEWIKKEDLLKLNLWEGDKIFLKLLQENAPFFSLKLVYKGDELTEAVLNGENLKK
                     S"
     gene            9983..11476
                     /locus_tag="ACZPCL_06985"
     CDS             9983..11476
                     /locus_tag="ACZPCL_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR1162244.1"
                     /translation="MKKHRSSQFLAICTAAVLALSVPAWASQVSITPLDTGNTTSSQS
                     QSGASEPSTAQNTSGQTVDISSITKPTVASQGAVLLNAADGSVLFSKNGDTTYYPASI
                     TKLMTALLVAENCNLDDTVTFSATATTNLESGAVSIGMAEGDTMTVRQCLYALLLKSA
                     NEVGNALAEHVAGSNAAFAEKMNAKAASLGCTNTHFANPHGLNDPNHYTTPHDMALIA
                     RAAFANDIVKTVASTRTYTLPATKNNPSGLTVTIGHKMLNPNDARYYQGIIGGKTGYT
                     SKAGNTLVTVAERNGVRLVAVVMKSQSTHYTDTKALLDYGFKLAEAGALTGQSTSQNN
                     TQVSGPASGKNASDNSSSSSNQAQETQGTTVTPAGQETAASTPVHAVSSGNNTGGPGV
                     TKERGWVKDDTGWYYVKNNGVKASNEWITDNGDEYWIQEDHYMAKGWKEINGKWYYLC
                     STGSMAKNVWKTGAEGNWYYLGPDGVMLTNTMTPDGYRVGADGVCIQ"
     gene            complement(11522..12427)
                     /locus_tag="ACZPCL_06990"
     CDS             complement(11522..12427)
                     /locus_tag="ACZPCL_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HIR1162245.1"
                     /translation="MEFKQLEAFVAVVDYKSFSEAAKHLYLTQPTISSHIQALEKELN
                     SRLLIRTTKQMKVTDRGMQLYECAVHMLNMRDQITDEFVGNKWKLIDVSASTIPSSYL
                     LPELLSEFSKIQPDIHFHIWQSDSADAIEKVIQGAVDFGLTGSKTDAEDCCFIPFCTD
                     SLVMAAPVTPEYLELADTLKKEEPVALKHFLSHPFILRANGSGTRKEIDLFLERHQIN
                     LSDLNIVARMNDLESIKKSIVSGLGVSILSARSVKDMERTHQLLTFPLESAAQVRTFY
                     IVYSKNRILKPYVKQFLKFVEHYYK"
BASE COUNT         3661 a         2582 c         3251 g         3225 t
ORIGIN      
        1 gatcgagatc agaggaaaga ccattacaga gacaaaagag gatgatcgaa agatcgtagg
       61 ttttgtccgg gatatccatg aaacaaagat gcgggaactg aaacaggaac gaaagcagat
      121 gatagatcct gttaccttat tttaccgttt aaaaccggga atggaggtaa ttgagaaggt
      181 tcgtggaaag tatccccagg gtatgtttat tcttgtggat atctgtcatt ttaccatcat
      241 aaccagggac tttggtctga catttaggga tgtggtcctg gaagaatttt ccaaaatgtt
      301 tctggaggtg tgtgccgaag atctttccag acctccggtg atgatccgtg caggttctga
      361 tgaactcctt gcctggatcc ctaaaattac agaggaaagc tgcaggcaga aactggaaaa
      421 actgaaagaa cgtttttctg tgatgatccg gcgcagtgcc ttaaatctta atttccaggc
      481 tggaattgcc agaggggatg aaagtgtaac aaccacctct ctgttaagac gggtccggat
      541 cgctgttaca gaagcggaaa agcaggagat cggtgtcctt tcctggaatg aggtaaagga
      601 tccgggagca cagccgaaaa catttggaaa gatcgtttcc atgggatata tggaacagat
      661 cagtctggct tccctggcat taaatctgtt agaccgctgt ccgtctattg atgcaggact
      721 ggatctgctt tcccgccgct taaaagcacg ttatggaatg gaaaatatgc tgatcaccag
      781 ttttcattat gatttccttt ccagcgagct ggaataccag tggaagcatc ttcatggcct
      841 catgggaaaa cggggcgttg tccactgtac agaagaagag tatgaaaaat taaatcagga
      901 tgcccagatc aacgcagtgc agcctgtaaa acagctgttg tccgttgtac ctgttttcag
      961 gaaaacagat atagaccaga ccggtgtggg gatccttatg tcagataacg gacagtattc
     1021 cggaagcatc ttccttttgg gaatagaacc atctgtttat gaaaatgagg aggatagaaa
     1081 gcttctctgg gagatcggca ttatcatcca gaatcgtatc aacagagacc gtcatgatca
     1141 gtctgcacag gcaaaatccg atttcctggc ccatatgtcc catgagatcc gtactcctat
     1201 gaacggtatc attggtatga cagagatcgc tctaaaagaa ggacagacag aggaagtgcg
     1261 tttagactgc ttaaagaagg taaaaggatc ctccaaatat ctgttaggtc tgttaaatga
     1321 tattttggat atgtcaaaga tcgaaagcgg aaagatgaaa ctggtaaaag atgatttcca
     1381 gcttgataag atgatcggcg aactgtatcc gttattggaa gctaagtttg aggaaaaatc
     1441 tcagaaatat caccaggaga tccatttggt caaccagtgg ttctatgggg atgctttgcg
     1501 tctcagtcag gtcctggtca atctgttaag caacgcagtg aaatattccg gggatcacac
     1561 ggagatcact ttaacagtag aggaaacaag gctttctgat ggtctttcta aattacagtt
     1621 tgcggtgaag gatcacggca ttggtgtcag tgaagaagac cgccagcgta ttttcagaag
     1681 ttttgagcag gtggaccgtt ctgcggtagg acagcaggga accggactgg gactggcgat
     1741 cagcaaccgc ctggtacata tgatgggcgg aatcattgag cttgaaagcc agatcgggga
     1801 aggaagtacc ttccggtttt ccattacgtt aaagccggtc agcgcccacc atgtaaaaga
     1861 agaagtttcc agagtggaaa gagattttac aggtgtccgc gttctggtaa cagaggacaa
     1921 tccattgaac cgggagatat tacagtatat tctggaagat atgggaatgt ctgtggaaaa
     1981 tgcctgtgac ggaaaagagg cggtagataa gtttaccgcc tccgagaaag gatattatga
     2041 tctgatcatt atggatatta tgatgccggt tatgaacgga ctggaggcag cccatgccat
     2101 ccggaccacc gatcatccgg atcataaaaa gatcccaatc attgccatca gtgccaatgc
     2161 ttttgatgaa gatatccgca ggtctcttgc aagcggcatg aatgcccatc tttccaagcc
     2221 aatcgaagtg gataaactgc gggagaccct tggaaaactg ttaaaataag gacttactga
     2281 acaaagggag caatggtttc ttccggggct ggctgtgagg tttccggtgc agccggagca
     2341 gttggcgctg gctctgttgg tgcaggagcc gtctgtgcag gggctgtctc atgttttgga
     2401 gcctttttct ctgttgtccc agccttagtt ggagcagctg cttttgttgg atttgcaaca
     2461 gaactgcttt cttgtgctcc ttttgtgctg gaggcagttg tatcagaagt agttgtaccg
     2521 gaagtagttg tgccggaagc agaagaggtg ttgcggtgga tcacagcact atggccaaga
     2581 taggttttgg agtggtccag agtacgttcg acttcctgtc cgtctttgta tacaactnnn
     2641 nnnnnnngta tcccagcggc tgcctttggt ggccgctttt attgtatttt ctgttcccgg
     2701 agctaaagta gggtcttcta cgtattccgg ttccggcggg tcgatttctt ctactttttc
     2761 agaagccaga tcccatacaa ccccatcctc taatacaggc acgccgaaaa tagaaacagt
     2821 tgccttgcgg tcagcgtagc ttgcaagaat accgataggt ttgtcagaag tatttacaaa
     2881 acggaagtca ggctgttccc agctgatggc agcatcctgg cccggagtta catagttagg
     2941 ctcgaaagta tgggagcggc ggaaggtaac ttccatgccg gactgaaata ctacccggta
     3001 taaggtactg gagatctggc atacaccgcc tcccagttcc tggacgacag cgccgctgtt
     3061 gtatgcggct gcttcctggt atcctttttc cgcagtacgc tgtcctacaa cagagttaaa
     3121 agaaaattct tccccgggct ggacaatggt tccgtttaaa gcttctgcag caaggcgtac
     3181 attggtgttg cgcttatcat tgtccgtggt ttcagtggta aaagtgacga gagtcttgta
     3241 gctggctttt acctcatcac aggaaataga aggtgcttcc ttatgggact gtacttctac
     3301 tacagcatcc atgcggtttt catgaatggc agctaaaata tcattggcca gctgatcccg
     3361 gtcaatacgt gagccggcgc agccttccct gaacacaaac tgtcccttct ggttgtcata
     3421 gtggtccagg gtactgatct ttgaaggaac atcccattct tttgccagtt cttccagttc
     3481 ctgggaaatg gaatttttca gggcatccgt tggaagctct gccgctgata atgtaagatc
     3541 tcctgaaacg gtttcctctt ctttactggt aagccagtcg cggatggcgt ctgtaagaat
     3601 gttgggaagt tccttactgt catcttcata tgcggcagtc atcttccagg aaagagactt
     3661 actgatccgg gaaacagcct catctttgct taatccctct aaagaaatgc cgttgacagt
     3721 cagtaagaca ggagtagttt cagatgcctg gctggcagtt gcttttgcgg ctccttccgg
     3781 ttcagaaaca gaacgggaat ggagtacagc agcggagatg cctaagagta cgacaacagc
     3841 tcctgctgct gcaaagggga tccaacctcc ttttattggt tttcttttcg gcttgtcata
     3901 gtcaaaattc agccgctttg gtgtatgatc cttatcccgt cttcctctgg gaccatgata
     3961 ggaagagcgg gaattcttgt ttaaagacat aaatatccct cttttcatat gctttttcat
     4021 atgctttata aagtatttta cagattttgt attatataag aagggataag ggatgtcaag
     4081 gcaggtaaat actggtttaa ggaaatatta aactaaatgt caaaatgtct ggaacaggcg
     4141 ggtcaggacc gtaaaaataa tacagcataa agcaccgcaa aatgccggca gaaaagcaga
     4201 aacaaaggtc caccatttac tttttgtttc tttatacagt gtgatcaggg tagtggaaca
     4261 gggccagtgc atcagggaga aaagcaggac gcagacagct gttttccagg tccagccatg
     4321 taacagaagg atctctttta actgtcccat attttcaaaa tttgttaggc ttccctgggc
     4381 tgtgtaggcc ataaggataa taggaagaac gatttcgttt gccggaagtc ccaggataaa
     4441 ggcagttaag atcaccccgt ccatccccat aagacgtcca agagggtcta aaaaccggct
     4501 gaaacgtgca agaaggctta tatttcccgg agctgtattt gccatgatcc acaggagaaa
     4561 accggcagga gcagctacag cggcagctct tccaaggacg aaaagggtcc ggtctgttac
     4621 cgagcgaaag atcactttgc cgatctgggg cttcctgtaa gggggaagct ctaaggtaaa
     4681 ggaagaggaa actcctttta agatcgttgc agataaaagg taagaggtaa taaaagtcat
     4741 ggcgatccct gaaaggatga acaaagtaag cagcagggca ctggcagcag aagaaagcag
     4801 gccatggcta tttccggata aaaacagcag gatcaaagtg ataaaaagag gcagacggcc
     4861 gttacaggga acgaaactgt ttgtgatcat ggcaataagc cgttctcttg gggagtcgat
     4921 aatacgacag cctgtgatcc ctgctgcgtt gcagccaaat cccattgcca tggtaagagc
     4981 ctgtttcccg caggctttac agcactggaa aggccggtct aagttgtagg cgatacgggg
     5041 cagatagccg gagtcctcca ataaggtaaa aagaggaaag aaaatagcca tgggaggcag
     5101 cataacggaa accacccagg cgaggacctg atagacccca tgaacgaagg cgtcccggat
     5161 ccagtctgga aaatgaaggg ataaaagggc ctgatccagt accggctgaa aggaaaaaaa
     5221 cagtctggaa agcagggcgg acaggtcgtt tgccccaacc agggtgatcc agaagataag
     5281 ggctaaaagt ccggccatgg caggataggc agccaggcgg cctgtaagga tgcggtccag
     5341 tttccggtcg gtttcattgt agctgcgttc cggcagcttt acacaaaggg aggcaatggt
     5401 ttcagcgttg tcgatcaggc cggagacaat gcggtctttt aatatgtctt taaaaatgcc
     5461 ttttctgttt aaatgatccc aggcagtttt taaacagtct gcaatggcag gatcctgtga
     5521 cggaacaagc tggcaggcat ttgtaaaaga ccggagaaag gacggatcat cttctagcag
     5581 gcgcagggag agaaagcggg aagtggtata aaatggaagt tcatgggtat ccagccaatt
     5641 tttcacagca ggttccacca tggagactgc tttttccagt tcagggcagt aagtgacctg
     5701 atagtgggga atggcaggac cttcagtaat atcatctaac cgctccaaga aagaagaaag
     5761 tgtgcttttc tttctggcag aggttcctgc taccggtaca cacagcaggt ctgataactg
     5821 atccagatca atggtgatgt ttttcttttc cgcttcatct aaaagattga cacataccaa
     5881 tgtacggtcc gtagcttcca ggatctgaag gaccaggttt aaattgcgtt ccagacaggt
     5941 ggcatcacag acaatgacca ctgcctgatg agcaccgaaa cagataaaat ccctggcaac
     6001 ttcttcctct gcagaacggg aagagaggga gtaagttcct ggaatgtcta ccagtgtgta
     6061 atcacgggcc ggactgtggc agattcccca ggcattagct gtggtcttgc cgatccagtt
     6121 tccggtgtgc tgcttcttcc cggtagtaag actgttaaat aaagtacttt ttcctacatt
     6181 gggatttcct gcaagggcga tcacaggata gtgatccggt atggcttttg gctgcttcat
     6241 gctttctcct ttctgcagat ctggatgttt ctgctgtctg ctttacggat agcgatcaca
     6301 gctccgcgga tctggtaggc agcaggatcc cctaaagggc ttatcccaac acattctacc
     6361 agtgtatttt caacgagacc tatatcaaga aaacggcgtc ggatgctgcc ggaagaggta
     6421 aggcagcaga cccgtgcttt ttcaccaggt tttatatcgt tcagacaaag aggggaagtc
     6481 ataagaatat tacctgcctg ggaagttatt tgtacttatt atatgatacc aagggtcaaa
     6541 aggttaaaaa tccgatgcaa gcagtgaaaa tagctggaaa acttaagaaa gatgggttat
     6601 aatattaaca atataggtta taaaaaatac aacaggagaa ggcagaatta tcatggaaga
     6661 aaattatatt gaatggctgg taaaacggaa agatcccgta tatgcctggc cggtaaagat
     6721 cctgatgatc gttgtgtgcg cgttatcagt actggcagca ctgcagatgt tattgggaat
     6781 tctgatcttg gcggcagcag cagcggctac gtattttgtt tttctgaatt tgagtgtaga
     6841 atatgaatac ctgtttgcag atggcggttt cagtgtagac agtattcttg gaaaagcaag
     6901 aagaaagaaa acgtttgact gcgataaaga agatgtacag gtgatcgcac cggcaaattc
     6961 ctatgtttta aaggattatg aaaagcaggg aatgaaagtg atcgactgta ccagtcataa
     7021 cgccggagca gatgtctatg ccctgatcag ccagaaaggg gcccagacca ccaaggtatt
     7081 atttgaaccg ggtgataaga tgaaggcagc gatgcgcagg gtatttccaa gaaaatttat
     7141 ctagtgataa gtttacctca taaaaaatca taaggaattt caatgaatat tttccgttaa
     7201 atgggtttga caatccatat aaaatttgct agtattattt actaataaga aggccaaaac
     7261 gccagtgtct gataaaaccg atgttttttt agcgctggcg ttatattgaa ccttcataca
     7321 aaaattttac agagaaagag aggaaaaatg aatgggtaca attatcggtg aaggcattac
     7381 ctttgatgat gtgcttctgg ttccatccta ttcagaagtg atcccaaacc aggttgattt
     7441 aagcacaaac ctgaccaaga agatccgtct gaacattccg ttaatgagtg caggtatgga
     7501 taccgttaca gagcatcgta tggcaatcgc catggctcgt cagggaggaa ttggcattat
     7561 tcataagaat atgtctattg aggcacaggc tgaggaggta gatcgtgtaa aacgttctga
     7621 gaacggtgtt atcaccgatc cattctatct ttccccagag catacattaa aggacgccaa
     7681 tgatctgatg gcaaaattcc gtatttccgg tgtgccgatc acagaaggca agaagttagt
     7741 tggtatcatt accaaccgtg acttaaagtt tgaggaagat ttcagccgtc cgatcagaga
     7801 gtgcatgact tccaagaacc tggttacagc aaaagaaggc gttaccttaa aggaagcaaa
     7861 agcgatcctt gcaaaggcaa gagtagagaa gctgcctatc gttgatgatg atttcaacct
     7921 gaagggcctg atcaccatca aagatattga aaagcagatc aaatatcctc tgtctgcaaa
     7981 agatgcccag ggacgtctgt tatgtggtgc tgctatcggt atcacagcta acgttctgga
     8041 gcgtgtagaa gcactggtta aggcaaaagt agacgtagtt gttttagact ctgctcatgg
     8101 tcattccgca aacgttatcc gctgcgtaaa gatgatcaag gaagcatatc cggatgttca
     8161 ggtcattgca ggaaacgtag caaccggtga tgcaaccaga gccctgatcg aagctggcgc
     8221 tgactgcgtt aaggttggta tcggacctgg ttctatctgt actacccgtg tagttgcagg
     8281 tatcggtgtt cctcagatca ccgctgttat ggactgctac gcagttgcaa aagagtacgg
     8341 tatcccgatc atcgcagatg gcggtatcaa gtattccggt gatattacaa aggccatcgc
     8401 agcaggcggc agcgtttgta tgatgggaag catgcttgca ggatgcgacg agagcccggg
     8461 tgctttcgaa ctgtatcagg gacgtaagta taaagtatac cgtggcatgg gctctattgc
     8521 agcaatggaa aacggaagca aggaccgtta cttccagact gacgcaaaga agctggttcc
     8581 agaaggtgta gaaggccgtg tagcttataa gggtatggta gaagatactg tattccagct
     8641 gttaggcggt ttacgttccg gtatgggtta ctgtggagca aaggatatcc caactcttca
     8701 ggagacagga agatttgtta agatctccgc agcatcctta aaagagagtc atccacatga
     8761 tatccatatc acaaaagaag ctccaaacta ttccattgag gaatagatat gagtgcagaa
     8821 aaaaacaagc ggctgcgtta gcagacattt gtttttttca agccattcac gaagattcag
     8881 cctgcgtgag cagacagtgg agcgcgtcag cgcgggaatc tgagtgttag acgaaattca
     8941 tgggtcgccg cgtttgcggc ggctcttttt gggttatggg ccaaaagccg cagaccgggc
     9001 tttggctcat tgtaatgtat ttgaaaaaga aagcaggaac ataaattggg agaacgctcc
     9061 aaacttacaa cattatgtta tatagaaaaa gatgacagtt atctcatgct tcacagagtc
     9121 agcaagaaaa atgatgaaaa caaggacaaa tggattggga tcggcggcca ttttgaagaa
     9181 ggggaaagcc cagaggactg tcttttaaga gaagcaaaag aagaaaccgg tcttacactg
     9241 acttcctgga aattccgggg gatcgtaacc tttgtttccg atgtgtgggg aacagaatac
     9301 atgtgcctgt atacagcaga cggatataca ggggagatga tctcctgcaa cgaaggcgtt
     9361 ctggaatgga tcaaaaaaga agatctgtta aaactgaacc tgtgggaggg cgataagata
     9421 tttttaaaac ttttacagga aaatgcaccg tttttctcct taaaactggt gtataaaggg
     9481 gatgaactga cagaagctgt gttaaacggg gaaaacttaa agaaatcgta acaaaatggc
     9541 ttgttttttt gtaactgcgt gtgtgctata atgtaatgag cgcagggaaa aacaagcggc
     9601 tgcgcagcag acatttgttt ttcacaagcc attaacgaac gaaccgcctg cgttagcagg
     9661 cagtggagcg cgtatgcgcg ggggtcgtga gtttaagatg taatgagcgc agggaaaaac
     9721 aagcggctgc gcagcagaca tttgtttttc acaagccatt aacgaacgag ccgcctgcgt
     9781 cagcaggcag tggagcgcgt atgcgcgggg gtcgtgagtt taagatgtaa tgagcgcagg
     9841 gaaaaacaag cggctgcgca gcagacattt gttgccaagc atgtcgagga ggcgccagtg
     9901 acgcgtccgc tgacttttca caagccatta acgaaggtcc cgcctaagtt tctttcaaac
     9961 accacagaga ggacatatcc caatgaaaaa acatagatca tcccagtttc ttgccatctg
    10021 tacagcggct gttcttgcgc tgtctgtacc agcatgggca agtcaggttt ccattactcc
    10081 tttagataca ggaaatacaa cttcttccca aagtcagtcc ggggcttcag aaccttccac
    10141 agcgcagaat acttccgggc agacagtgga catttcttct attacaaagc cgaccgttgc
    10201 atcccagggc gcagttttat taaacgcagc agatggaagt gtgctttttt ccaagaatgg
    10261 ggatacaacc tattaccctg ccagtattac caagctgatg accgctcttt tagtagcaga
    10321 aaactgtaat ctggacgata cagttacctt ttctgcaaca gctactacaa acctggagtc
    10381 aggagctgtt tccattggca tggcagaagg ggatacgatg acggtacgcc agtgcctgta
    10441 tgcactgctg ttaaaatctg ccaatgaagt gggaaatgcc ctggcggaac atgtggctgg
    10501 cagtaatgca gcatttgcgg agaaaatgaa tgcaaaagca gcctctttag gctgtaccaa
    10561 tacccatttt gcaaatccac atggtttaaa tgatcccaac cattacacca cacctcacga
    10621 tatggctctg atcgccagag ctgcttttgc aaatgatatt gtaaaaacag tggcatctac
    10681 cagaacctat acacttccgg ctacaaaaaa caatccttcc ggtcttaccg tgactatagg
    10741 acacaaaatg ttaaatccaa atgatgcccg ttattatcag ggaattattg gtggaaagac
    10801 aggatatacg tccaaggcag gaaataccct ggtgactgtt gcagagcgca acggggtaag
    10861 gctggtcgca gtggtgatga agagccagtc tacccattat acggatacca aggcattgtt
    10921 agattatgga tttaagctgg cagaggcagg tgctcttaca ggacagagta ccagtcagaa
    10981 caatacgcag gtttccggcc cagcttctgg aaagaatgcg tctgataatt ccagcagttc
    11041 ttctaaccag gcacaggaaa cacagggaac gacagttact cctgcaggtc aggaaacagc
    11101 agccagcaca ccggtgcatg ctgtatcttc aggaaataat accggaggtc cgggcgttac
    11161 caaagaacgt ggatgggtga aagatgatac cggctggtat tatgtgaaaa acaatggagt
    11221 aaaagcttct aatgaatgga tcacagataa tggagacgaa tactggatcc aggaagatca
    11281 ctacatggca aaaggctgga aagaaatcaa tggtaaatgg tattacctgt gttctacagg
    11341 aagtatggca aagaatgtat ggaagacagg ggcagaaggt aactggtatt acctgggtcc
    11401 ggacggagtc atgcttacaa ataccatgac tccggatggt tacagagtag gtgcagacgg
    11461 tgtatgtatc caataatgtt tgtaccggtg ttattgtcag gcaacctgtt tatgtagttt
    11521 gtcatttata ataatgttct acgaatttta aaaactgctt tacatagggt tttaatatcc
    11581 tgtttttgct gtatacgatg taaaaagtac ggacctgcgc agcactctcc aatggaaagg
    11641 tgagaagctg gtgggtcctt tccatatctt ttacactgcg ggcagagaga atggaaactc
    11701 caaggccgga tacaatagat tttttaatag actccaggtc attcatccgg gctacgatgt
    11761 taagatcgga aagattgatc tggtgccgtt ctaaaaacag atcgatctcc ttgcgggtgc
    11821 cggaaccgtt ggcgcgaagg ataaaaggat gggataaaaa gtgttttaaa gccacaggtt
    11881 cctctttttt taatgtatca gccagttcca ggtattccgg tgttaccggg gctgccatga
    11941 ctaaagagtc ggtacagaag ggaatgaaac agcagtcctc tgcatctgtt ttagaacctg
    12001 taagaccaaa atccacagcg ccctggatca ctttttcaat ggcgtcagcg ctgtcagact
    12061 gccagatatg aaaatggata tccggctgta ttttggaaaa ctctgacaaa agctccggaa
    12121 gaaggtagga agaggggatc gtggatgcac tgacatcgat cagtttccat ttattcccaa
    12181 caaactcatc cgtgatctga tcccgcatat ttaacatatg gactgcacat tcatagagct
    12241 gcattccacg gtctgtaact ttcatctgtt tggtggtacg gatgagcaga cgggaattca
    12301 gttctttttc taatgcctgg atatgggaac tgatcgttgg ctgggtcagg tagaggtgtt
    12361 tggctgcctc ggaaaagctt ttataatcta caacggccac gaaggcctct aattgtttga
    12421 attccatggg tgtgactcct tttttcttca ctcattacat tatagcataa tttatagata
    12481 aaacctataa atacccacaa aatcatccaa tatctgatta gatattgaga aaaattaatg
    12541 ttaaaatgaa aacaaaaaga tttaaggggt gaagtgacat gtatcaggtg gggatcgata
    12601 taggctccag tgcggcaaaa accgtggtgc tgcgggatgg agaaatatgg aagaccatgg
    12661 agatggcaac aggattttcc agcaggaaaa ctgcagataa gatctcaaat gatcctgcag
    12721 tacaggaag
//
LOCUS       DBMDUA010000070        12529 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2606_length_12529_cov_2.893145, whole genome shotgun sequence.
ACCESSION   DBMDUA010000070 DBMDUA010000000
VERSION     DBMDUA010000070.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 12529)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12529)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12529
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2606_length_12529_cov_2.893145"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="ACZPCL_06995"
     CDS             <1..349
                     /locus_tag="ACZPCL_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HIR1162246.1"
                     /translation="DMQTIMAGLACGEPNTISWDILKNHVKVFIAAPDWVAANGMRML
                     AAPVKGDAPITSGESGAAPFGALAAMMTMDEYKDLRNDIGLDENSKVLLFSTEGDTDP
                     DRYKNIVWKGLDK"
     gene            397..1713
                     /locus_tag="ACZPCL_07000"
     CDS             397..1713
                     /locus_tag="ACZPCL_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HIR1162247.1"
                     /translation="MNLDYAKIKEAAQNYQKDMTKFLREIVKNPGESCDEKAHITRIA
                     EEMRKLDFTKVEIDPMGNVLGYMGTGKTLIAFDAHIDTVGIGNRNNWNFDPYEGYENE
                     TEIGGRGVSDQCGGIVSAVYGAKIMKDLGMLSDKYTVLVTGTVQEEDCDGLCWQYIIN
                     EDKVRPEFVVSTEPTDGGIYRGQRGRMEIRIDVKGVSCHGSAPERGDNAIYKMADILQ
                     DVRALNENDAADDKEVKGLVKMLDKKYNPEWEEANFLGRGTVTVSEIFFTSPSRCAVA
                     DSCAVSLDRRMTAGETWESCLDEIRALPAVKKYGDDVKVSMYEYNRPSYTDLVYPIEC
                     YFPTWVIPKDHDVTKALEEAYHNLYGEERIGAEETLAMRTARPLTDKWTFSTNGVSIM
                     GRNGIPCIGFGPGAEAQAHAPNEKTWKQDLVTCAAVYAALPTVYCK"
     gene            1813..3006
                     /gene="ygeW"
                     /locus_tag="ACZPCL_07005"
     CDS             1813..3006
                     /gene="ygeW"
                     /locus_tag="ACZPCL_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HIR1162248.1"
                     /translation="MKTLQDYIDKLKALNFKEMYEGDFFLTWEKTDDELEAVWTVADA
                     LRYMRENNISTKVFESGLGISLFRDNSTRTRFSFASACNLLGLEVQDLDEGKSQVAHG
                     ETVRETANMISFMADVIGIRDDMYIGKGNAYMHEVVDSVTQGYKDGILEQKPTLVNLQ
                     CDIDHPTQCMADMLHIIHEFGGVENLKGKKLAMTWAYSPSYGKPLSVPQGVIGLMTRM
                     GMEVVLAHPEGYEVMPEVEEVARKNAEKSGGSFRVSHDMADAFKDADIVYPKSWAPFA
                     AMEKRTNLYAEGDTDGIKALEKELLAQNAEHKDWCCTEELMKTTKDGKALYLHCLPAD
                     INGVSCKDGEVEASVFDRYRDPLYKEASYKPYVIAAMIMLAKFADPAEILKKLEEKGT
                     PRVFK"
     gene            3127..4080
                     /gene="arcC"
                     /locus_tag="ACZPCL_07010"
     CDS             3127..4080
                     /gene="arcC"
                     /locus_tag="ACZPCL_07010"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIR1162249.1"
                     /translation="MENKKKKRIVIALGGNALGNTLPEQMKAVKITAKAIVDLIEEGC
                     EVIVAHGNGPQVGMINNAMAALSREDANQPNTPLSVCVAMSQAYIGYDLQNALREELY
                     NRNIYDIPVATMITQVRVDADDPAFDAPSKPIGHFMTEEQAKIAEEKYGYIMKEDSGR
                     GYRRVVASPKPAEIVEIGAIRSLVDSGQLVIACGGGGIPVTRQGNHLKGASAVIDKDF
                     ASELLAENLDADFLIILTAVEKVAINFGKPEEKWLDDLETEEARKYIKEGHFAPGSML
                     PKVQAAVKFAESKPGRTALITLLEKAKDGIQGKTGTHIHLA"
     gene            4099..5427
                     /locus_tag="ACZPCL_07015"
     CDS             4099..5427
                     /locus_tag="ACZPCL_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIR1162250.1"
                     /translation="MNTTSKEGSLFELDGVPKLSQAFPLALQHVVAMIVGCVTPAIII
                     SGAAGLETADRVLLIQASLVVSALATLLQLFPIGKKTGFHLGAGLPVIIGVSFAYVPS
                     MQAIADQGGIPAILGAQIVGGVCAILVGLTIKKIRKFFPPLIAGTVVFTIGLSLYPTA
                     INYMAGGTGSATYGSWQNWLVAIFTLVVVTGLNHFAKGFLKLASILIGMLIGYIFSSF
                     FGMVSFSNVAQAGVFQLPQVLHFGITFEPSACIALGLLFVINSVQAIGDFSATTSGGL
                     DREPTTNELQGAILGYGVSNMVGACLGCLPTATYSQNVGIVATTKVVNRVTLGLAAVI
                     IFIAGVFPKFSALLTTIPYAVLGGATVSVFASIAMTGMKLVMTEDMNYRNTSIVGLAA
                     ALGMGVSQASASLSAFPAWVTTIFGKSPVVIATLVAIILNVTLPRDKKAN"
     gene            complement(5582..6340)
                     /locus_tag="ACZPCL_07020"
     CDS             complement(5582..6340)
                     /locus_tag="ACZPCL_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR1162251.1"
                     /translation="MKKTYRNMALAAACVLTATGLWGCSTSVQIPDTVKVQQGDSSDN
                     RITVSGMEEVKAVPDMAQIQYSVYTQAATAQECQEENAKNVNQTLETLKGLGVEEKSI
                     QTSDYGLSPIYDWNSGRQKITGYQMSTSITVSDIPVENAGKIITASVASGVNELDSVQ
                     YLCSDYDEKYQEALKMAVEMAKNKADAMAEAAGMTVVKAVNISEDGYYPQARYNTAGA
                     SAKQMMMEDAAADMGVMPGEVSIEAQVSVTFEME"
     gene            6628..6967
                     /gene="ssrA"
                     /locus_tag="ACZPCL_07025"
     tmRNA           6628..6967
                     /gene="ssrA"
                     /locus_tag="ACZPCL_07025"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(7041..7835)
                     /locus_tag="ACZPCL_07030"
     CDS             complement(7041..7835)
                     /locus_tag="ACZPCL_07030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0071554 - cell wall organization or
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR1162252.1"
                     /translation="MRDIALCHPRMQKIAAQWITACKAEGINVAISETLRTAAEQDAL
                     YAKGRTKPGNIVTNAKGSSYRSQHQWGIAFDFYLKMDVDGDGKIADDAYNDSKGHFRK
                     AAEIGKKLGLAWGGDWSSIVDKPHLYLPDWGSTPTPLIKQYKTPEKFMKTWIMEPVKM
                     GWQKENGGWRFYLKDGSENYVVNDWYKDGDLWYWFDGNGIMVHNVWYQYKGHWYYLGR
                     DGAMVKGLQTISGKWYYLDEDGRMATEPVVLTPQKDGALQYPKLTE"
     gene            complement(7852..8115)
                     /locus_tag="ACZPCL_07035"
     CDS             complement(7852..8115)
                     /locus_tag="ACZPCL_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR1162253.1"
                     /translation="MDFGIAGVAAITVICYLGGMACKTTEKVKDEVIPVVCGVTGGIL
                     GVAGMYLIPEFPAGDVINAAAIGIVSGLASTGVHQVIKQVGKK"
     gene            complement(8329..9606)
                     /locus_tag="ACZPCL_07040"
     CDS             complement(8329..9606)
                     /locus_tag="ACZPCL_07040"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR1162254.1"
                     /translation="MAAEKASRAKTTFLSNMSHDIRTPMNAIVRLTTLLEHEIHEPEK
                     LQLHIQKIQNSSQHLLGLINDILDMSKIESSEVKLNEDSVDLMEQVAQIDSIIRPQAE
                     ERGQEFKIHVHGIDHGHLLGDSVRLRQIFINLLSNAVKYTSVGGSVILDLTKEPSSIP
                     EHANISITVTDTGYGMTPEFIKTIFEPFTRAENSTTNKIQGTGLGMAITKSIVDLMGG
                     RIQVKSELNKGSCFEVVLPFAIDPNGSQEKNGKYLHQEGEKVSVLKGMKFLCAEDNEL
                     NAEILEAILEMYQASCKICPDGEKLVEAFETSAFGDYDAILMDVQMPNMNGLEATKRI
                     RNGKNPLGRTIPIIAMTANAFNSDVQECLDAGMDAHVSKPLDIGKIINSFKFPINLSL
                     KTRYEIGKNVIEEKIIDPSVEYKIQKVQDDDNV"
     gene            complement(9606..9737)
                     /locus_tag="ACZPCL_07045"
     CDS             complement(9606..9737)
                     /locus_tag="ACZPCL_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162255.1"
                     /translation="MAFYLGWVFLQPSEKEAFRNGRVQEIQKLNEELAASQENFRLL"
     gene            complement(9841..9975)
                     /locus_tag="ACZPCL_07050"
     CDS             complement(9841..9975)
                     /locus_tag="ACZPCL_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162256.1"
                     /translation="MREFWKKAIQVLLYGGLEKEQYKRISAEINEANRKSIVVLSTHQ
                     "
     gene            complement(9983..12115)
                     /locus_tag="ACZPCL_07055"
     CDS             complement(9983..12115)
                     /locus_tag="ACZPCL_07055"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012707.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding response regulator"
                     /protein_id="HIR1162257.1"
                     /translation="MKISGAHKAMKRVLIFLVTLGIIVPILFTAGQMGLFSPEPTVEI
                     EAGITSESAPVLRIATDYDFCPNSYYNKDGELSGLYVEIMIEVANRLGMRPEFKTADW
                     MGCRKMLTEDEVDVLTGLEIFSNMEGTLRTIPFCSDELRVYGRSRIDSAAALAGKKVA
                     LMARSVIATTYDLQCDYLEYSTNTEILEAVEQGEADFGICHGAVATKIIEKNHLHLVP
                     SLVITKSYPALAVHDTQPKLQRMINEVVRNMSEDGTIGRLQNKWITEFARNRSLEYVF
                     HQNEVFYITFILGIIIVLCITAGYWEVDRRQEKYIRTLLEYQEKLQQSNEETKRANQA
                     KSEFLSHMSHDIRTPINGIMGMVEIIKKNLDDPERIKDCLEKIDKASHHLLSLINDVL
                     DMSKIGSGKVHLEEIPVDLDEEMEKIHAIADVQAKKQEIRFSIEDEVVHRQFLGSPAH
                     LRRILLNLISNALRYNKKGGKICLAIREVEYDGSHIGLEFKVQDTGIGMSREFVEKSL
                     FKPFTQEDDRVRTEYRGTGLGMSIVYELVKQMNGTIDVNSKPGEGTTFTVKLAFKTVD
                     SAWKKKEIQGENRNITGMNILAAEDNQLNMEILQFLLEEAGAKVTAVSDGKQAVEYFA
                     DAASGTYDVILMDIMMPVMDGLEASKKIRELPEGKGKDIPIIAMTANAFVEDKEKTKE
                     AGMNAHLTKPVNREEIIRVLAAYWKNKG"
     gene            complement(12194..>12529)
                     /locus_tag="ACZPCL_07060"
     CDS             complement(12194..>12529)
                     /locus_tag="ACZPCL_07060"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase family protein"
                     /protein_id="HIR1162258.1"
                     /translation="RKSLYIKTTNLTKKYKHSCGLYAVFHSERCQTPGDLKKYRDPGF
                     LKEHPNVEIFYQGQWITCPIILAKVTDETDGTPYQKGERKILTLSTCYGTSKRMILQA
                     LNPVAGGMN"
BASE COUNT         3239 a         3043 c         2556 g         3691 t
ORIGIN      
        1 cgatatgcag accatcatgg caggtcttgc atgtggtgaa ccgaacacca tttcctggga
       61 catcttaaag aaccatgtaa aagtattcat tgctgctcct gactgggttg ctgcaaatgg
      121 tatgcgtatg ttagctgccc ctgttaaggg cgatgctccg atcacctccg gtgagtccgg
      181 tgctgctcca ttcggtgcat tagcagctat gatgaccatg gacgaataca aagatttaag
      241 aaatgacatt ggcctggatg aaaactccaa agtactgctc ttctctacag agggcgatac
      301 cgatcctgat cgctacaaga acattgtatg gaaaggcctt gacaaataac tattaaccat
      361 caaaagtaat tgcaaaacaa gaggaggata tttaaaatga atctggatta cgcaaaaatc
      421 aaagaagcag ctcagaatta tcagaaagat atgactaagt tcttacgtga gatcgttaag
      481 aacccaggtg agagctgtga cgagaaggct cacatcaccc gcatcgcaga agaaatgaga
      541 aagttagact tcaccaaagt tgagatcgac ccaatgggca acgtacttgg ctatatggga
      601 accggaaaaa ccctgatcgc atttgatgct catatcgata ctgtaggtat tggtaataga
      661 aataactgga acttcgatcc atacgaggga tatgagaacg agaccgagat cggcggccgt
      721 ggcgtatctg accagtgtgg cggtatcgta tctgctgttt acggcgctaa gatcatgaaa
      781 gacttaggca tgctgtctga caagtacact gtattagtaa ccggtactgt tcaggaagaa
      841 gactgtgacg gtctgtgctg gcagtatatc atcaacgaag acaaagttcg tcctgaattc
      901 gtagtttcca cagagccaac tgatggtggt atctaccgtg gacagagagg acgtatggaa
      961 atccgtatcg acgttaaggg cgtttcctgc catggttccg ctccagagcg tggtgacaac
     1021 gctatctaca agatggctga catccttcag gacgttcgtg ctctgaacga gaacgacgca
     1081 gctgatgaca aggaagttaa gggccttgtt aagatgttag acaagaaata caatccagaa
     1141 tgggaagagg ctaacttcct tggacgtggt actgtaactg tatccgagat cttcttcacc
     1201 tccccaagcc gttgtgcagt tgctgactcc tgtgcagtat ctcttgaccg tcgtatgacc
     1261 gccggagaaa catgggaaag ctgcttagac gagatccgtg cccttccggc tgttaagaag
     1321 tacggcgatg acgttaaggt ttccatgtac gaatacaatc gtccttccta cactgatctg
     1381 gtatatccaa tcgagtgcta cttcccaaca tgggtaatcc cgaaggatca tgatgtaacc
     1441 aaggctctgg aagaagctta ccacaacctg tacggtgaag agcgtatcgg tgctgaggaa
     1501 actctggcta tgagaaccgc tcgtcctctg actgacaagt ggaccttctc taccaacggc
     1561 gtatccatca tgggacgtaa cggaatccct tgcatcggct ttggaccagg cgctgaggct
     1621 caggctcatg ctccgaacga gaagacctgg aaacaggatc tggtaacctg tgcagctgta
     1681 tatgcagctc ttccaactgt ttactgcaag taatcaatta ttttagccag tctgcgtggc
     1741 ccttcggggc tgcgcttcca tcaagaaatt ctttaaattc cataaaccta catataaaag
     1801 gagaattgaa tcatgaaaac attacaggat tacattgaca agctgaaagc attaaacttc
     1861 aaagaaatgt acgaaggcga tttcttcctg acctgggaga agacagacga cgagctggaa
     1921 gctgtctgga ctgttgctga cgctctccgt tacatgagag agaacaatat ttctacaaaa
     1981 gtatttgaga gtggtctggg tatctcctta ttccgcgata actctacccg tacccgcttc
     2041 tcttttgctt ctgcatgtaa tctgcttggt ctggaagttc aggatctgga cgaaggcaag
     2101 tctcaggttg cccatggcga gactgtacgc gaaacagcta acatgatctc tttcatggct
     2161 gacgttatcg gtatccgcga tgatatgtac atcggcaagg gcaacgctta catgcatgag
     2221 gttgttgatt ctgtaaccca gggttacaaa gatggtatcc tggagcagaa gccaacctta
     2281 gtaaacttac agtgtgatat cgaccatcct acccagtgta tggctgatat gctgcacatc
     2341 atccatgaat tcggcggcgt agagaactta aagggcaaaa agctggctat gacatgggct
     2401 tactctccat cttacggcaa gccattatct gttcctcagg gcgttatcgg tctgatgaca
     2461 cgtatgggta tggaagttgt tctggctcat ccagaaggct acgaagtaat gccagaagtt
     2521 gaagaagttg ccagaaagaa cgctgagaag tccggcggtt ccttccgtgt atctcatgac
     2581 atggctgacg cattcaagga tgctgatatc gtttatccaa agagctgggc tccatttgca
     2641 gctatggaaa agagaaccaa cctgtatgct gaaggcgata ccgatggcat caaggctctt
     2701 gagaaagaac tgttagctca gaacgcagag cacaaagact ggtgctgtac agaagagctg
     2761 atgaagacaa ccaaggatgg caaggctctg tacctgcact gcttaccagc tgatatcaac
     2821 ggcgtaagct gcaaggacgg cgaggttgag gcttctgtat tcgatcgtta ccgtgatcct
     2881 ctgtacaaag aagcaagcta caagccatac gttatcgcag ctatgatcat gctggctaag
     2941 tttgctgatc ctgctgagat cttaaagaaa ctggaagaaa aaggaactcc aagagtattc
     3001 aaataaaatc tagttaaaag ataggtgttt ctgagctgcc ggaccggagc gcgtcgtaaa
     3061 tggctttctg tcagacgcac tcgggctacc ggtggctctt tttatcgaaa aaaggggtaa
     3121 ttaacaatgg aaaacaagaa gaaaaaacgt atcgtcattg ctctgggcgg caatgcttta
     3181 ggaaatactc ttcctgagca gatgaaggca gtgaagatca ccgctaaggc tatcgtagac
     3241 ctgatcgagg aaggctgcga agtgatcgta gctcacggaa acggtccgca ggttggtatg
     3301 atcaacaacg ctatggctgc attaagccgt gaagatgcaa accagcctaa cactcctctt
     3361 tctgtctgtg ttgcaatgag ccaggcatat atcggctatg acttacagaa tgctttaaga
     3421 gaagagcttt acaaccgcaa tatctatgat atcccggttg ctaccatgat cactcaggta
     3481 cgtgtagacg cagatgatcc tgcttttgac gctccatcca agccaatcgg tcacttcatg
     3541 acagaagaac aggctaagat tgctgaagaa aagtacggat atatcatgaa agaagactcc
     3601 ggaagaggct accgtcgtgt tgttgcttct ccaaagccag ctgagatcgt tgagatcggc
     3661 gcgatccgtt ccttagttga ctccggtcag ttagtaattg cctgcggcgg cggcggtatt
     3721 cctgttacaa gacagggcaa ccacttaaag ggcgcaagcg ctgttatcga taaagacttt
     3781 gcaagtgagc tgttagctga aaatctggat gcagatttcc ttatcatcct cactgctgtt
     3841 gaaaaagttg ctattaactt cggaaagccg gaagaaaaat ggctggatga ccttgaaacc
     3901 gaagaagcta gaaagtacat caaagaagga catttcgctc ctggctctat gcttccaaaa
     3961 gttcaggctg ctgttaaatt tgctgaatca aaaccaggac gcactgcgct cattacgctg
     4021 ttggagaaag caaaagacgg tatccagggc aagaccggta cccatatcca tttagcgtaa
     4081 ataaaggggg cattgcatat gaatacaacc agtaaagaag gatctttatt tgaattagac
     4141 ggcgtgccga aattaagcca ggcatttcct ctggcattac agcacgttgt ggcaatgatc
     4201 gtaggctgcg ttacacctgc catcatcatt tccggtgcag ccggtcttga aaccgcagac
     4261 cgcgtactgc tgatacaggc atctttagtt gtgtctgcac ttgctactct cctgcagctc
     4321 ttcccaatcg gaaaaaagac aggttttcac ttaggtgccg gacttcccgt tatcatcggt
     4381 gtaagtttcg cctatgtgcc aagtatgcag gccattgctg atcaaggcgg catcccggct
     4441 atcctgggcg cccaaatcgt aggcggtgtc tgtgctatcc ttgtaggact gacgatcaaa
     4501 aagatccgta agttcttccc acctttaatc gccggtaccg tagttttcac catcggcctt
     4561 tccctttacc caactgctat caactatatg gcgggaggta caggaagcgc tacttatggt
     4621 tcctggcaga actggctggt cgctatcttt actctggtag ttgttactgg tttaaatcat
     4681 tttgcaaaag gatttttaaa actggcatct atcctgatcg gtatgctcat cggatatatc
     4741 ttttccagct tctttggtat ggtaagtttc tccaacgtag ctcaggctgg tgtgttccag
     4801 cttcctcagg tcctgcattt cggcattact tttgaaccat ctgcatgtat cgccttagga
     4861 ctgctgtttg tcatcaactc tgtacaggct atcggtgact tctccgctac tacttccggc
     4921 ggtttagacc gtgaaccaac tacaaatgag cttcagggtg ctatccttgg atacggcgtt
     4981 tccaatatgg tcggcgcatg tttaggatgt cttcctactg caacttacag ccagaacgtt
     5041 ggtatcgttg ctactacaaa agttgtaaac agagtaactc ttggtcttgc agctgtgatc
     5101 atctttattg caggtgtatt tcctaaattc tctgctcttt taacaactat tccatatgca
     5161 gtactgggcg gtgcaaccgt ttccgtattc gcatccatcg ccatgaccgg tatgaaactg
     5221 gttatgacag aagatatgaa ctaccgcaat acctctatcg taggtcttgc agcagctctt
     5281 ggtatgggtg tttctcaggc gtctgcgtcc ctttccgcat ttcctgcatg ggtaacaact
     5341 attttcggaa aatctccggt agttattgct acattagtag ctattatcct gaatgtaaca
     5401 cttccaagag ataaaaaagc caattaatga tgttcagaat gtttaaaatg tttcattatt
     5461 aatctggtct cttttctatc ctcaaaagag ggatgtcacc tgacggcttt ttaagccggg
     5521 tgacatccct cttttaattt gtgtatctat tgactcacaa acacgcgcta acgcactcat
     5581 tctattccat ctcaaaagtt acgcttacct gggcttctat ggacacttct cccggcataa
     5641 cgcccatatc tgcagcagca tcctccatca tcatctgctt ggcagaagca cctgctgtat
     5701 tgtatctcgc ctgaggataa taaccatctt ccgatatatt tactgccttt acaaccgtca
     5761 ttcctgcagc ctctgccatg gcatctgcct tgttctttgc catttctaca gccattttca
     5821 atgcttcctg atatttttca tcatagtcac tgcaaagata ctgaacactg tccagttcat
     5881 tgacaccgga agctacagaa gctgtaatga tctttcctgc gttttctacc gggatatctg
     5941 aaactgtgat gctggtactc atctgataac ctgtaatctt ctggcgtccg gaattccaat
     6001 cgtaaatagg actcaaacca taatccgatg tctggatgga cttctcttcc actcccagac
     6061 cttttaaagt ctccagggtc tgatttacat ttttcgcatt ttcttcctgg cattcctggg
     6121 ctgtcgctgc ctgtgtgtat acactatact ggatctgggc catatccggc actgccttta
     6181 cttcttccat accgcttact gtaatacggt tgtcactgct atctccctgc tgcaccttta
     6241 cagtatccgg gatctgcaca ctggtgctgc atccccaaag tcctgttgcc gttaaaacac
     6301 aagctgccgc cagcgccatg tttctgtaag ttttcttcat aatacgtttc ctccttttac
     6361 tgttttcttc tcctttagag gacatctgct gacctctttt ataattctat tctagcagat
     6421 taagattatt tttgttttct tttctcttaa taatgatttg cctgtttatt acaaatacct
     6481 gacaaataaa tggcacatta tcttacacaa aatgcagaca attctcccct ctgctgtatt
     6541 gacataaaag aatgaaaatg atatgatact aagagtagca tttcttcgtt ggcctatcag
     6601 ataaacaaaa actgagtatt ccattcaggg gttgtactgg tttcgacggg ggtcatgcag
     6661 ctggtgaagc tatccccatg cgatgggtta aatggcaaaa ttaaaaataa acgctaacga
     6721 cgaattagca tacgctgcct aatggcagct gtcagactta aggcacctac actttaagac
     6781 cctggcatcg actatgtagg aaacgacaca tgcaaagctt tgcgcatgcg ggcgtattat
     6841 gaagctacta aaaccgtcag agtgtttctt cccggacggt agagggaatg tcaaataagg
     6901 aactatgata gtagaagaac agggaattga ttttcggaca ggggttcgat tcccctcagc
     6961 tccactaaaa aaaccgcgca aacacgcggt tttttctttt tccaatttaa cttgtattac
     7021 atttcatctt gcatatggtt ctattctgtc agctttggat actgcagcgc cccatccttc
     7081 tgcggcgtaa gcaccaccgg ttccgttgcc atccggccgt cttcatccaa ataataccat
     7141 ttcccgctga tggtctgcag tcctttcacc atagcgccat cccttcccag ataataccaa
     7201 tgtcccttat actgatacca cacgttatgg accattattc cattgccatc aaaccagtac
     7261 cagagatcgc catctttata ccaatcatta acaacataat tttccgaacc atctttcaga
     7321 taaaatctcc agccgccatt ctccttctgc catcccattt tcactggttc cattatccat
     7381 gttttcataa acttttccgg tgttttgtac tgtttgatta atggagtggg cgtacttccc
     7441 cagtcaggca gatacaaatg gggcttatct acaatgctgg accagtctcc gccccaggca
     7501 agaccaagtt tcttgccaat ttcagcagcc ttcctgaaat gacctttact gtcattataa
     7561 gcgtcatctg ctatcttccc atctccatcc acatccattt tcagataaaa atcaaaggca
     7621 atcccccact ggtgctgtga ccggtaggaa ctgccttttg cattggttac gatatttccc
     7681 ggttttgtac ggcctttggc atagagagca tcctgttctg ctgcagtacg cagtgtttca
     7741 gaaatagcaa cattaattcc ttctgccttg caggcggtga tccactgagc tgctatcttc
     7801 tgcatccgcg ggtggcacaa tgcaatatct ctcatataca cggtacctcc cttatttttt
     7861 ccccacctgc ttgatcacct ggtgtactcc cgttgaagca agcccggaaa cgataccaat
     7921 agctgctgcg ttaatcacat caccagccgg aaactccggt atcagataca tgcctgcaac
     7981 ccccagaatt ccgccggtta cgccacagac taccggtatc acctcatcct ttactttttc
     8041 cgtcgtctta cacgccattc cgcctaaata gcagatcacc gtgatcgccg ccacacctgc
     8101 aatcccaaaa tccatatcct cttcctctct ttccagcaat atttatgggg atatcttcca
     8161 ttgtcctcat aatttatcat atgctggaac agcctaaaag gaccgatcag aaatattttc
     8221 tgtatttttg aaatctacag aaatggcaaa tactcctact agtttttcta aatatgtaat
     8281 taaacatatt tttccaaata taatggaaat acttttaaat tttacatttt atacattatc
     8341 atcatcttgc accttttgaa ttttatattc tacagaagga tcaattattt tctcttctat
     8401 cacattttta cctatttcat atctagtttt taaagataaa tttataggga atttaaaact
     8461 atttattatt tttcctatat ccaacggctt ggacacatgt gcatccatac cggcatctaa
     8521 acattcctgt acatctgaat tgaatgcatt ggctgtcata gcaataatgg gaatggtcct
     8581 gcccagagga tttttgccat tacggatcct tttcgttgct tccaggccat tcatatttgg
     8641 catctgtacg tccattaaaa tagcatcata atctccgaaa gcagaagttt caaaggcttc
     8701 aaccagtttt tcaccgtctg ggcagatctt gcaggatgcc tggtacattt ccaaaatcgc
     8761 ttctaagatc tccgcattca gttcattatc ttctgcgcaa agaaatttca ttccttttaa
     8821 tacggaaact ttttcacctt cctgatgcag atattttcca tttttttcct gtgaaccatt
     8881 gggatctatt gcaaaaggaa gtaccacttc aaaacagctt cctttattta actcgctttt
     8941 cacctgtatc ctgccgccca tcaggtcaac gatacttttc gtgatggcca tacccagtcc
     9001 tgttccctgt attttgttgg tcgtggaatt ttctgctctg gtaaaaggtt caaagatcgt
     9061 ttttataaac tccggcgtca ttccatatcc ggtatctgta actgtaatgg atatattggc
     9121 atgttccggt atactgcttg gctcttttgt cagatccagg ataacgcttc ccccaacaga
     9181 tgtatattta acagcattgg acagcagatt gataaagatc tggcgcaggc gcacagagtc
     9241 tcccagaaga tgtccatgat cgatcccgtg aacatggatc ttaaactcct gtccccgttc
     9301 ttctgcctgt ggacggatga tactgtctat ctgtgccacc tgctccatca gatcaacgga
     9361 atcttcattt aatttcacct cgctggattc aatcttactc atatccagaa tgtcattgat
     9421 aagtcccagc agatgctgac tggaattttg gatcttctgg atgtggagct gcagtttttc
     9481 cggctcatgg atctcatgtt ccagaagagt ggtaagtctg acaatggcat tcataggagt
     9541 ccggatgtcg tggctcatat tacttaaaaa tgtggttttc gcccggctgg ccttttcagc
     9601 agccactaaa gcagcctgaa gttttcctgg ctggcagcca gttcttcatt taatttttgt
     9661 atttcctgaa cccgtccgtt cctgaatgct tccttctccg atggctgcaa gaatacccac
     9721 cccagataaa atgccataaa catacatgcg caaacatgaa ccagaaacgg attgtttctg
     9781 atcctgaaat ttacaaatgc caacattcca aataaaagga tggctcccat aaatatgatc
     9841 ttattggtgt gttgacagca ccacaatgct ttttctgttc gcctcgttga tctcagcaga
     9901 gattctctta tactgttctt tttccagacc tccatacagc aggacctgga tggccttctt
     9961 ccaaaactct ctcatgtact cgttaacctt tatttttcca atatgctgcc agaacccgga
    10021 tgatctcttc cctgtttaca ggctttgtca gatgggcatt cattcccgcc tcttttgtct
    10081 tttccttatc ttctacaaat gcattggccg tcatcgctat gatcggaatg tcttttccct
    10141 tgccctccgg cagttcacgg atcttttttg acgcttcaag accgtccatg accggcatca
    10201 taatatccat caggatcaca tcataggtgc ctgatgctgc atccgcaaaa tattctactg
    10261 cctgttttcc gtctgatact gccgttactt tcgctccggc ttcttctaac aggaactgaa
    10321 ggatctccat atttaactgg ttatcttctg cagccagtat gttcatcccg gtaatattcc
    10381 tgttttctcc ctgtatttcc ttctttttcc atgcagagtc cactgtctta aatgccaact
    10441 ttactgtaaa tgttgtacct tcccccggtt tgctgtttac atcgatcgta ccgttcatct
    10501 gctttaccag ttcataaaca atggacatcc caagaccggt tccccgatat tcggtcctta
    10561 cacggtcatc ttcctgtgta aatggcttaa acaggctttt ttccacaaat tcccggctca
    10621 taccaattcc tgtatcctga accttaaact ccagaccgat atgggatccg tcatattcca
    10681 cttcccggat ggccagacag atctttcccc cttttttatt ataacgcagg gcattgctga
    10741 tcaaatttaa aaggatcctt ctcaaatggg ccggactgcc aagaaactgt ctgtggacaa
    10801 cttcatcctc aatagaaaac cttatttcct gcttcttcgc ctgtacatct gcaatcgcat
    10861 ggatcttttc catctcctca tccagatcca ccggtatttc ttccagatgg accttcccgc
    10921 ttccgatctt gctcatatcc aaaacatcat tgatcaggga cagcagatga tgcgatgctt
    10981 tatcgatctt ttccagacag tctttgatcc gctccggatc atccagattt ttcttgatga
    11041 tctccaccat acccatgatc ccgttgatcg gtgtgcggat atcgtgggac atatgggaga
    11101 gaaactcact tttggcctga ttggcccgtt tcgtctcttc attggactgc tgcagttttt
    11161 cctgatattc caatagggtg cggatatatt tttcctgacg tctatctact tcccagtaac
    11221 cggctgtaat acacagtaca atgatgatcc ccagtataaa tgtaatataa aatacttcat
    11281 tttggtggaa tacatattct aaggacctgt ttctggcaaa ttccgtgatc catttatttt
    11341 gcagccttcc aatagttccg tcttcagaca tattccggac cacttcattg atcatgcgct
    11401 gcagttttgg ctgtgtatca tgaactgcta aagccggata actctttgtg atcacaaggc
    11461 ttggcaccag atgcagatga tttttttcaa tgatctttgt ggcaactgca ccgtgacaga
    11521 tcccgaaatc tgcttctccc tgctctactg cctccaatat ttccgtattg gtgctgtatt
    11581 ccagataatc acactgcaga tcataggtgg ttgcgatcac agaacgcgcc attaacgcaa
    11641 ccttcttacc tgcaagggca gccgcactgt cgatcctgct tctgccatat acacgcagtt
    11701 catccgaaca gaaagggatc gtccttaaag tcccttccat attggaaaat atttccagtc
    11761 ccgtcaaaac atctacttcg tcctcagtaa gcattttccg gcagcccatc cagtcagccg
    11821 ttttaaactc cggacgcatt cccaagcggt ttgccacctc tatcatgatc tcaacataaa
    11881 gacctgataa ttccccatcc ttattgtaat aggaatttgg acagaaatca taatctgttg
    11941 cgatccgcaa aaccggcgct gattcacttg taataccagc ctctatttct actgtaggct
    12001 ctggtgaaaa aagccccatc tgcccggctg taaaaaggat cggaacaata attcccaaag
    12061 taacaagaaa gatcagcacc cgcttcatcg ccttatgtgc cccacttatc ttcatttctc
    12121 acccatgctt tcttttccat tttcagctac gtatatctaa ctgatttaat tatactgtcc
    12181 agcaaatttc ttgtcaattc attccccccg caaccggatt aagtgcctga aggatcatcc
    12241 gtttactggt tccataacag gtggataagg tcagtatctt tctttctcct ttttgataag
    12301 gtgttccatc tgtctcatct gttacttttg ccaggataat ggggcaggtg atccattgcc
    12361 cctgataaaa gatctctaca ttgggatgtt cctttaaaaa tccgggatcc ctgtattttt
    12421 tcagatcacc gggagtttga cacctttccg aatgaaaaac agcgtataag ccgcatgaat
    12481 gcttatattt ttttgtcaaa tttgttgttt ttatatatag gctttttcg
//
LOCUS       DBMDUA010000071        12508 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2608_length_12508_cov_2.800677, whole genome shotgun sequence.
ACCESSION   DBMDUA010000071 DBMDUA010000000
VERSION     DBMDUA010000071.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 12508)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12508)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12508
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2608_length_12508_cov_2.800677"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="ACZPCL_07065"
     CDS             complement(<1..784)
                     /locus_tag="ACZPCL_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162259.1"
                     /translation="MADRNEADALFATKRKKQQEEQAEQERREEMSRKKGEMEAEIRR
                     LEEEARRQKEKQEEDRRQAEEEARRVKQEAEEAEARAKRAQEMAEEAKRKQEEVRREE
                     ERRVQEKREEEKRARERAAQEKAQAKEAAKAETRNGVKEKPARPGRDKADIQPGKKLP
                     VLPLAIGGVAVIAVLVICLVLFSGGKKGGVFGTDCDRMAAKQVLSYDVFYPESFEEAY
                     DDGGVYLKYGSVENGDFSFVYVLGSTQEDMNYMTESTDPTDLI"
     gene            complement(838..1089)
                     /locus_tag="ACZPCL_07070"
     CDS             complement(838..1089)
                     /locus_tag="ACZPCL_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HIR1162260.1"
                     /translation="MERKYILAVKPLEPYILQIDFISGSRLLLDMKPYVDKIRFRTLT
                     DPKVWNSAVTNGIFVRFGDVELSHDEILSMAEREHLNWQ"
     gene            complement(1238..3739)
                     /locus_tag="ACZPCL_07075"
     CDS             complement(1238..3739)
                     /locus_tag="ACZPCL_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007037914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1162261.1"
                     /translation="MPRKKNDLNTIYISERLQEKLRPISRCTLTTVIAPMGYGKTTAV
                     NWYLNERVKKDHGVVIRISIYTNNLPIFWKSVQNAFRFAGFDFFEEYECPSDPASGGL
                     FADDLSHLLAGETDYYIFIDDFHLLTDARISEFLCTLSRRIPENVHLVVAGRDRFLSG
                     GAVVGLGGKLHQITVDHLRLNHTELSVYAHLCGTQLSDRQIESLLHSSEGWFSAVYLN
                     LCAFAEQGELPDNHSDIYEMFSAAMIDPLPEIQREFLVVMGLADEFSAEMAKFITENE
                     DTKQLLSAMTKQNAFVSRLTDGVTYRFHHMMKECAERAFMAMDKEKQTIYLDRYGKWY
                     EEHKQYLHALDSYRKSGNFDAALRVIRKDAGILLASLKADDVLNFLSKCPKEDLEAHP
                     LSILVLMRCMFNWRKIQEMLKLKDFLIQVIERHTEISMEERGNLLGECDLITSFLMYN
                     DISKMSHFHRSASRQMSRPAISIRNDGGWTFGSPSVLMMFHREPGSLLKEQEEMNECM
                     PHYYKITNGHGQGAERVMDAEALFIQGCLSDAQIALEGAYARIEENGQECMALCCDFL
                     SRRLSLCMNIKQRYSFEQRYMDLMKHHNSMWINIFNSTSAYYYALIGRVEKIPEIFGE
                     HRLFAVNFLAPGRPMMEMIENQVYLAQGAYAKVIGRSEGILAVCSAMHYALVALHVQI
                     QTAAAYERIGKHGEARTILKQELSDASKDHMVMPFVENFIYLSSLFEGKFPEIDQEFL
                     AKILSLGKAYEENCKKMGKEKSYPEQFKCLTEQELKLTELMAAHMSNKEIAARLYLSE
                     GTVKQYINQIYSKLEITGDTRTKRKQLLEQFYTNN"
     gene            3983..5968
                     /gene="uvrB"
                     /locus_tag="ACZPCL_07080"
     CDS             3983..5968
                     /gene="uvrB"
                     /locus_tag="ACZPCL_07080"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR1162262.1"
                     /translation="MDHFKLVSEYKPTGDQPQAIEQLVKGFKEGNQFETLLGVTGSGK
                     TFTMANVIEQLNRPTLIIAHNKTLAAQLYSEMKEFFPENAVEYFVSYYDYYQPEAYVP
                     STDTYIEKDSSINDEIDKLRHSATAALSERRDVVIVASVSCIYGLGSPIDYQNMVISL
                     RPGMEKDRDDVIKKLIEIQYDRNDMDFKRGTFRVKGDVVDIFPAYSDSEAYRIEFFGD
                     EVDRIMEIDTVTGEIKAQLGHVAIFPASHYVVPKEKMLEATEHILEELKERVAYFKSE
                     DKLLEAQRISERTNFDVEMMRETGFCSGIENYSRHLTGLAPGEPPCTLIDYFPEDFLI
                     IVDESHITLPQVRGMYAGDRSRKSTLVNYGFRLPSALDNRPLNFEEFESKINQMMFVS
                     ATPSDYERDHELLRAEQIIRPTGLLDPEISVRPVEGQIDDLISEVNKETSAHHKVLIT
                     TLTKRMAEDLTDYMREAGIRVKYLHSDIDTLERAQIIRDMRLDVFDVLVGINLLREGL
                     DIPEITLVAILDADKEGFLRSETSLIQTIGRAARNSEGHVIMYADTITDSMRKAIDET
                     ERRRRIQQKYNEEHGITPTTIKKAVRDLIAISKAVENPHAADTKDPESMDEKELKKLA
                     KELEKKMRQAAAELNFEEAANLRDRMIEVKKMLLDIL"
     gene            6162..7040
                     /locus_tag="ACZPCL_07085"
     CDS             6162..7040
                     /locus_tag="ACZPCL_07085"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1162263.1"
                     /translation="METKLVLLGTGTPNACPNASGPASAVVVGDRAYLVDFGPGVVRQ
                     ASKAYFNGIDALRPDRLTVAFSTHLHTDHTAGYPDLIFTQWVLERPVPLKVFGPKGLK
                     HMTEHILMAYETDIDFRIHGFEKANEQGYQVDVTEIEEPGIVYKDDRVTVEAFPVSHG
                     TLISYGYKFVTADRTIVISGDTAPLDIVAEKAAGCDILLHEVEYSAGIACREPKWQKY
                     HQEVHTLSTDLAKVAQKAKPKLLVTYHRIYHMNIQDNHINLEAEMRRRDDAILDEIRA
                     AGYEGMTVNGRDLEVF"
     gene            7040..7951
                     /locus_tag="ACZPCL_07090"
     CDS             7040..7951
                     /locus_tag="ACZPCL_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1162264.1"
                     /translation="MKKALFYGILASFFFAFTFLLNRSMHLAGGYWLWSACLRYLFTF
                     PILAAVLAISGKKQKTRPLSHTWAEITKAPGEWFLWSSVGFVLFYAPLTFGSTFGESW
                     LAAATWQLTIVAGILLTPLWGKPIPIRNLSWSCLILAGVFLLQVPNMRQMKLETMALT
                     LIPILIAAFSYPLGNRKVMSLVSSDISTLERVYAMTLCSLPVWIFLSIWAFITHGLPG
                     RDQLIQSFFVALFSGTAATVLFFKATDMVKHNHKWLAVIEATQSGEVIFTLLGGILFL
                     KDPVPSAIGLAGIAIIVAGMTGNSLCQ"
     gene            complement(8002..8577)
                     /locus_tag="ACZPCL_07095"
     CDS             complement(8002..8577)
                     /locus_tag="ACZPCL_07095"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1162265.1"
                     /translation="MTRQQLTDAIEKYRPYNEQEEMDQPLILNWIKNEKDAFLRENKI
                     AHITASAWVVNKDRSKVLMVYHNIYHSWSWLGGHADGETDLLSVAIREVKEEAGISNV
                     YPVSEEIFSLESLTVDGHVKKGKYVSSHLHLNVTYLLEADPKENVSVKADENSGVAWF
                     SPEEALGKSTEPWFVEHVYKKLINKVKMDTM"
     gene            complement(8610..9944)
                     /locus_tag="ACZPCL_07100"
     CDS             complement(8610..9944)
                     /locus_tag="ACZPCL_07100"
                     /EC_number="2.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050277 - sedoheptulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sedoheptulokinase"
                     /protein_id="HIR1162266.1"
                     /translation="MQIIGIDIGTTTISGAVLEYENEKTVKLLEAKTIENGGFMPTAN
                     EWEKIQDAGAIVKKAEKLLDDLLDKYPQTERIGLTGQMHGIVYIDQTGTCVSPLYTWQ
                     DGRGSLCEKGQCSLVQEIKEKCDLEAASGYGLVTHICNLKHGLVPERAVGFCTIMDYF
                     GMCLTGRKKPLVHVSNAAGFGFFDGRNMKFETEKLSKMGVKETWLPEVCTGIEKLGLY
                     RNRQVTTAIGDNQASFLGAAGKEENTLLVNMGTGGQISILSDQYFAEKGIEARPFLDG
                     KYLLVGSSLCGGKSYALLEQFFRRVVKVVTGEDSSLYPVMEQFAKTGKEQSNRISEDR
                     KLKIRTTFDGTRTDPKSSGSIQGLTSDNFTPEAFTYGILEGMGDELYQMYRTIKNGTG
                     IQIKNLIGSGNGVRKNPVFCEMLEELFQNKLVLSAYKEEAAAGAAISSMLCS"
     gene            complement(9994..10521)
                     /locus_tag="ACZPCL_07105"
     CDS             complement(9994..10521)
                     /locus_tag="ACZPCL_07105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HIR1162267.1"
                     /translation="MNEIMNVMKERRSIRKFKPDMPSKEDLNQIIEAGLYAASGMGKQ
                     AAIIVAVTNKELRDKLSEMNRRIGGWEEGFDPFYGAPAVLVVLADKNCPTHVYDGSLV
                     MGNMMLEAKALDLGSIWIHRAKEEFELPEYKQLLKDLGVEGEWEGIGHCVVGYADGDL
                     PKAAPRKENRVFWVE"
     gene            complement(10596..10829)
                     /locus_tag="ACZPCL_07110"
     CDS             complement(10596..10829)
                     /locus_tag="ACZPCL_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162268.1"
                     /translation="MSKVIIAIVAMFALMIFPGAGFWNLLSNVVGGGVAESFLYPIYG
                     GLILLAGFVVGCTVLILEEIQSLKEKIDDLSNK"
     gene            complement(10826..12157)
                     /locus_tag="ACZPCL_07115"
     CDS             complement(10826..12157)
                     /locus_tag="ACZPCL_07115"
                     /inference="COORDINATES: protein motif:HMM:NF022641.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HIR1162269.1"
                     /translation="MKEKKRVWFTFNLCEADAFEQYLEKMALQGWFLESVGGAVMRFY
                     RAQPEKRRYAALLVPESSSLIGADDWKAEQLREQCEEAGWIFQCNSIYWHIFYTTDDA
                     AKRRGDMEKERQFQIQKALSWNWSVKFFYPILVVLEIWAVYQYLQNPGKLFADPMQLL
                     LTLLLTGMIISWAATYIRLFRWSHGNMAAIKKGEPLTEQDLKRTLKCKKYTFMGDGIL
                     ILGVIALAFLSSMEALISFILSSTLIVLIALFVRKWVRENGSGSNRDDWMTYLVGVGV
                     ACMILIPLCNGAATHFFSEEEPDTGRKQTILASYEEGDPSGKSIDRPIGVTVYTSPIP
                     WIINKTSKCYPKDMTKLWDCTRQEIPAEIGALPEDMEVSWYRYMVRKDGIRSGGQEGN
                     DPQEDAYEAEPAVDEVILKDKRRLVVLDYGGGTDLDGIKEAVDAFKEGNIQ"
BASE COUNT         3191 a         3159 c         2433 g         3725 t
ORIGIN      
        1 gtataagatc agtaggatct gtgctttctg tcatgtaatt catatcttcc tgtgtacttc
       61 ctaacacata aacaaaggaa aaatccccgt tttctacact gccatatttt aagtagacac
      121 cgccatcgtc gtatgcttct tcaaaacttt ccgggtaaaa tacatcgtag cttaacacct
      181 gttttgctgc catacgatcg cagtctgtac caaatacacc accctttttg ccgcctgaaa
      241 acagtaccag acagataaca agtacagcta tcacagctac accgccgata gctaacggta
      301 gtacgggaag cttctttccc ggctgtatgt ctgctttatc tcttcctgga cgtgctggtt
      361 tttcttttac tccgtttctg gtctctgctt tcgcagcctc ttttgcctgg gctttttcct
      421 gtgctgcccg ctctcttgcc cgtttctcct cttcccgctt ttcctggacc cggcgctctt
      481 cttcccgcct tacctcttcc tgctttcttt tagcttcttc tgccatttcc tgagctcttt
      541 tagccctggc ttctgcttct tctgcctctt gctttacccg tctggcctct tcttctgcct
      601 ggcgcctgtc ttcttcctgt ttttctttct gccgtctggc ctcttcttct aaccgccgga
      661 tctctgcttc catttctccc tttttccggc tcatttcttc ccttcgttcc tgctctgcct
      721 gttcttcctg ctgttttttt cttttagtag caaataaagc atccgcttcg ttccgatccg
      781 ccatactcct tctcctctct cttttctccc gatcatgtgt gacacgttaa aaaataatta
      841 ctgccagttc aaatgttccc gttctgccat ggaaaggatc tcatcatggc tcagttccac
      901 atcgccgaac cggacaaata tcccatttgt tacggcactg ttccagacct ttggatctgt
      961 aagggtgcgg aaccgtattt tatctacata aggcttcata tccaaaagca accggcttcc
     1021 tgatataaag tcgatctgca ggatataagg ttctaagggc ttaacagcca aaatatactt
     1081 tctctccaca tgagtcctcc ttccaagttg aataaagatc agcatctacg tgcgtgcacg
     1141 caatctgacc agccactcca caacactttc atactaaaaa agatctaccg tatctttact
     1201 gtacgataga tccttctgaa aaaatattaa ctaaaggtta gttattggta taaaactgtt
     1261 ctaaaagctg tttccgcttg gttcgggtat cccctgtgat ctctaattta gaatagatct
     1321 ggttaatata ttgctttacc gttccctctg aaagataaag ccttgctgcg atctccttat
     1381 tgctcatgtg ggctgccatt aattctgtca gtttcaactc ctgttctgta agacatttaa
     1441 actgctccgg ataagacttt tcttttccca tttttttaca attttcttca tatgcttttc
     1501 caagcgaaag gatttttgca agaaattcct ggtctatttc cgggaatttc ccttcaaaga
     1561 gggatgaaag atagataaaa ttttccacaa aaggcatgac catatgatct ttggaagcat
     1621 ctgaaagctc ctgttttaaa atcgttctgg cctccccgtg ttttccgatc ctttcatagg
     1681 ccgctgcagt ctggatctgc acatgaaggg caactaaagc ataatgcata gcactgcaca
     1741 ctgccaggat cccttcactt ctcccgatca ccttggcata ggctccctgg gctaaataca
     1801 cctgattttc gatcatttcc atcatcggtc ttcccggcgc caggaaattc acagcaaaaa
     1861 gtctgtgttc tccgaatatc tccgggatct tctccacacg gccgatcaat gcataataat
     1921 aagcgctggt gctgttaaaa atattgatcc acatggaatt atgatgcttc atcagatcca
     1981 tataacgctg ttcaaaggaa tacctctgtt taatgttcat acacaacgac agccgccggg
     2041 acagaaaatc gcagcacaat gccatacatt cctgaccatt ttcctcgatc cgggcatagg
     2101 ctccttccaa agcgatctgg gcatctgaca aacacccctg gataaacagt gcctctgcat
     2161 ccatgacccg ttctgccccc tgaccatgac cattggtaat cttataataa tggggcatgc
     2221 actcattcat ttcttcctgc tcttttaaaa gactgcctgg ttccctgtgg aacatcataa
     2281 gtacagaggg cgaaccaaag gtccagccac cgtcattgcg gatactgatg gcaggccgcg
     2341 acatctgcct gctggcgctg cggtggaaat gactcatttt tgaaatgtca ttgtacatca
     2401 gaaaactggt gatcaggtcg cactctccta agagatttcc ccgttcttcc attgatattt
     2461 ccgtgtgtct ttcaatgacc tggatcagaa aatcttttaa ttttaacatc tcctggatct
     2521 tacgccagtt aaacatacag cgcatcagca ctaaaatcga taacggatgg gcttctaaat
     2581 cctcttttgg gcatttactt aaaaagttta atacatcatc cgcctttaaa gatgccagga
     2641 gaattcccgc atctttccgg atcactctga gggctgcatc aaaatttccg cttttcctgt
     2701 aagagtccag tgcatgcaga tactgcttat gttcttcgta ccatttgcca tagcggtcca
     2761 gataaattgt ctgtttctct ttatccattg ccataaaggc ccgctctgcg cattccttca
     2821 tcatatggtg gaaacggtag gtcactccgt ctgtaagacg gctgacaaag gcattctgct
     2881 tggtcatggc agacaacagc tgttttgtgt cttcattttc cgtaataaat ttggccattt
     2941 ctgcagaaaa ttcatctgca aggcccatta caacaaggaa ttccctttgt atctcaggga
     3001 gcggatcgat catagctgct gaaaacattt cataaatatc ggaatgatta tccggcagct
     3061 ctccctgttc cgcaaaggcg cataaattca ggtaaacagc ggaaaaccag ccttcactgg
     3121 aatgaagaag tgactcgatc tgccggtcgc ttaactgtgt accgcaaaga tgggcataga
     3181 cagaaagttc tgtatggttt aaccgcagat gatccactgt gatctgatgc agcttgccgc
     3241 ctaagccaac cactgcaccg cctgataaaa aacggtcccg gccggccact accagatgaa
     3301 cattttccgg gatccgcctt gataatgtac agaggaattc actgatcctt gcatccgtaa
     3361 gaagatgaaa atcgtcaata aaaatataat aatctgtctc gcctgccaga aggtggctga
     3421 ggtcatctgc aaacagaccg ccgctggccg gatccgaagg gcactcatat tcctcaaaaa
     3481 agtcaaaacc tgcaaaccga aaggcattct gcacgctttt ccagaaaatc ggaaggttat
     3541 tggtataaat gctgatgcgg atcacaaccc catgatcctt tttcacccgc tcattcagat
     3601 accagtttac agccgtggtc ttcccatagc ccataggagc tatcaccgtt gtcagggtac
     3661 accgggagat cggccgcagc ttctcctgca aacgctctga tatgtaaatg gtgttcagat
     3721 catttttctt tcttggcatt ttcatttccc tcccccggtc tttctatatc cattttacca
     3781 tataatatag gtttggggaa gaatattgtt tattcaccag cagaacaaat gttctttatt
     3841 atatctatac tttttataaa atgcatggta tactttttat aaattttaat ttatgaagaa
     3901 aaatagatac acctgcaact gtcgagtaat tctcgactgt tcaaaatgat atgaaaaatt
     3961 ttaagcaact aaggagcatt tcatggatca ttttaaatta gtatctgaat ataaacccac
     4021 cggtgaccag cctcaggcca tcgagcagct ggtaaagggc tttaaagagg gaaatcagtt
     4081 tgaaactctt ttaggtgtta ccggttccgg taagacattt accatggcaa atgtgatcga
     4141 gcagctaaac cgcccgacgc tcatcattgc ccacaataaa acgctggcag cccagcttta
     4201 ttctgaaatg aaggaatttt tcccggaaaa cgcagtggaa tactttgtct cctactacga
     4261 ttattatcag ccggaagcct atgttccttc tacggacact tacattgaaa aggactcttc
     4321 gattaacgat gagattgata agctgcgtca ctctgccact gctgctcttt ctgagcgccg
     4381 ggatgtggtg atcgtggctt ctgtttcctg tatttatggt ctgggaagtc ccattgatta
     4441 tcagaacatg gttatttccc tgcggccggg tatggaaaaa gaccgggatg atgtgatcaa
     4501 aaagctgatc gagatccagt atgaccgcaa tgatatggat tttaagcgtg gtactttccg
     4561 tgttaaagga gatgtggtcg acattttccc ggcttactcg gacagtgaag cctacaggat
     4621 cgagtttttc ggtgatgaag tagaccgcat tatggaaatt gatacagtaa ccggcgagat
     4681 caaagcccag ttaggccatg tggctatttt ccctgcctcc cactacgttg ttcccaagga
     4741 gaagatgtta gaggcaacag agcacatttt agaggaatta aaagaacggg tggcctattt
     4801 taaaagcgaa gacaagcttt tagaggcaca gcgtatttcg gaacgcacca attttgatgt
     4861 ggaaatgatg cgggaaaccg ggttctgctc tggtattgaa aactattcca gacatttaac
     4921 cggtctggca ccaggagaac cgccctgtac actgatcgat tatttcccgg aagatttcct
     4981 tattattgta gatgagtccc atattacact gccccaggtc cgtggcatgt atgccggtga
     5041 ccgctcccgt aagtctactc tggtaaacta cggtttccgc ctgccctctg cccttgacaa
     5101 ccgaccttta aattttgagg aatttgaaag taagatcaac cagatgatgt ttgtgtctgc
     5161 tactccttct gactatgaaa gagaccacga actgctccgt gcagagcaga tcatccgtcc
     5221 taccggactt ttagatccgg agatctctgt gcggcctgta gaaggacaga tcgatgacct
     5281 gatctctgaa gtaaacaaag aaacgtccgc ccatcacaaa gtccttatca ccactcttac
     5341 caaacgaatg gcagaagatc ttacagatta tatgcgggaa gctggtatcc gggtcaaata
     5401 tctccactca gatattgata ctttagagcg ggcacagatc atccgggata tgcgtctgga
     5461 tgtatttgac gtcctggtgg gtattaacct gctgcgtgaa ggcttggata tcccggagat
     5521 cacactggtt gccattctgg atgcagataa ggaaggcttc cttcgttcag aaacttccct
     5581 gatccagacc atcggacgtg ccgcccgaaa ctcggaaggt catgtgatca tgtatgctga
     5641 caccattact gactctatga gaaaagccat tgatgaaaca gaacgccgcc gcaggatcca
     5701 gcagaaatac aatgaagaac acggaattac tcctactacc atcaaaaaag cggtccgtga
     5761 tctgattgct atctccaaag ctgtggaaaa tcctcatgct gccgatacca aagatcctga
     5821 gtccatggac gaaaaggaat taaagaagct ggcaaaggaa ctggaaaaga agatgcgcca
     5881 ggctgctgca gagctgaact ttgaggaagc tgcaaatctg cgcgaccgta tgatcgaagt
     5941 aaagaaaatg ctgcttgata tactatgaaa gtgttgtcaa gcgactatgg ggactgcgtg
     6001 cttgcacgca aggaaccata gacatttgac aaccaagccg gtatgagtaa gcagggcgac
     6061 agcccggatt acgaatgctg gcggcagttg tgggagtgcc agcggcacgt aacaactgac
     6121 ttactttcat atatagtaaa atacacagga gaatatatat tatggaaaca aaacttgtcc
     6181 ttttaggaac cggaacccca aatgcctgcc ccaatgccag cggacctgct tctgctgtag
     6241 ttgttggtga cagagcctac cttgtagatt ttggccccgg cgtggtacgt caggcttcca
     6301 aagcttattt taacggcatt gatgcccttc gcccggaccg ccttaccgtg gctttttcca
     6361 ctcatctgca tacagaccac actgccggat acccggacct tatttttact caatgggtat
     6421 tagagcggcc tgttccgtta aaagtttttg gcccaaaggg cttaaagcac atgaccgaac
     6481 acattttaat ggcctatgaa actgatattg atttccgtat ccatggcttt gaaaaggcaa
     6541 atgaacaggg ctatcaggta gatgtaacag aaattgaaga acctggtatt gtttataaag
     6601 atgaccgtgt cactgtagag gcctttccgg taagccacgg aaccctgatc agctacggct
     6661 ataaatttgt cactgctgat cgcaccattg tgatctccgg agatactgcc ccgttggaca
     6721 ttgttgctga aaaagccgct ggctgcgata tccttctcca tgaagtagaa tacagtgccg
     6781 gtatcgcctg cagggaacca aaatggcaga aataccacca ggaggtccac accttaagca
     6841 cggacctggc aaaagtggcc cagaaagcaa agccaaaact tcttgtcact tatcaccgca
     6901 tctaccacat gaatatccag gataaccaca tcaatctgga agcggaaatg cgacgcaggg
     6961 atgatgccat cttagatgag atccgtgctg ccggttatga gggaatgact gtgaatggac
     7021 gggatttgga ggtattttaa tgaaaaaggc actgttttac gggatacttg cttcattttt
     7081 ctttgctttt acatttttac taaaccgtag tatgcatctt gcaggaggat actggctatg
     7141 gtctgcatgc ctgcgttatc tttttacctt tcctatcctt gctgcggtac tggcaatttc
     7201 aggaaaaaag caaaaaacaa ggcctctttc acatacctgg gcagaaataa caaaagctcc
     7261 cggagaatgg tttctctgga gcagtgttgg ttttgtactg ttttatgcgc ctttaacttt
     7321 tggctcaact ttcggtgaat catggctggc agcagcaacc tggcagctga ccattgttgc
     7381 cggtatttta ttaacacctt tatggggtaa accaataccg atcagaaacc tctcctggtc
     7441 ctgcctgatc ctggccggtg tttttctcct tcaggttccc aatatgcgac aaatgaaact
     7501 ggagacaatg gcattaacac tgattcccat tctcattgca gctttctcct atcccctggg
     7561 gaaccggaaa gtcatgagcc tggtttccag cgatatctct accctggaac gggtctatgc
     7621 catgacttta tgtagccttc ctgtttggat ttttctttca atatgggctt ttatcactca
     7681 cggactgcct ggaagagatc aactgatcca gtccttcttt gtagcgcttt tctccggaac
     7741 cgctgctact gtcctgttct ttaaagccac tgacatggta aaacacaacc ataagtggct
     7801 ggcagtgatc gaagctactc agtccggaga agtgatcttc acactcttag gcggtatcct
     7861 gtttttaaaa gatcctgttc cttccgcaat cggactggct ggtattgcca ttatcgtagc
     7921 aggcatgacc ggaaacagtt tatgccagta aacaaaaagc tgactctgct aaaaaacagg
     7981 tcagcttttt gccattttta attacattgt atccatcttt actttattta tcagtttttt
     8041 gtatacatgc tccacaaacc atggttccgt ggactttccc agtgcttctt ccggagaaaa
     8101 ccaggctact ccactgtttt cgtctgcttt tacggaaaca ttctctttcg gatctgcttc
     8161 taacaggtat gttacattta agtgcagatg gctggatacg tatttaccct tttttacatg
     8221 accatcgaca gtaagtgatt ccagggaaaa gatctcttct gatacaggat aaacattgga
     8281 aatacctgct tcttctttca cttcccggat ggcaaccgac aacagatctg tctccccgtc
     8341 tgcatggcct cccagccagg accaggaatg atagatgtta tgatacacca taaggacttt
     8401 gctcctgtct ttgtttacca cccaggcaga agcggttata tgagcgatct tattttccct
     8461 tagaaatgca tctttctcat ttttaatcca gtttaagatc agtggctgat ccatttcttc
     8521 ctgctcatta tagggtctgt atttttctat tgcatctgtt aactgctgtc tggtcattcc
     8581 gcttctctcc ctgtaacttc ttatcttttt taactgcaca gcatgctgct tatagcagcc
     8641 ccggcagccg cttcttcctt ataagcagac agtaccaatt tgttttgaaa taattcttcc
     8701 agcatttcac aaaatacagg attttttctc accccattgc ctgaaccgat gagatttttg
     8761 atctggatac cggttccatt tttgatcgtc ctgtacatct gatataattc atcacccata
     8821 ccttccagta ttccataggt gaacgcttca ggagtaaaat tgtcagacgt taaaccctgg
     8881 atgcttccgc tgctttttgg atctgttctt gtgccgtcaa aggttgtcct gatcttaagt
     8941 tttctatctt cagagatcct gttgctctgt tcttttcctg tttttgcaaa ctgttccatc
     9001 accggataaa gagagctgtc ttctcctgta acaaccttta cgacccttct gaaaaactgt
     9061 tccagcagtg cataggactt tccgccgcat aaagaagatc ccaccaacag gtattttcca
     9121 tccagaaatg gcctggcttc gatccccttt tctgcaaaat actgatcgga aaggatcgag
     9181 atctggccgc ctgttcccat attgaccagc agggtatttt cttcttttcc cgctgccccg
     9241 agaaaactgg cctgattatc tcctatggca gtagttacct ggcgatttct gtaaagtccc
     9301 agcttttcaa tccctgtaca tacttcagga agccaggttt cctttactcc cattttgctt
     9361 aatttttctg tttcaaattt catgttccga ccatcgaaaa atccgaagcc cgctgcattg
     9421 cttacatgta ccagtggctt tttccggcca gtaagacaca ttccaaaata atccataatg
     9481 gtgcaaaaac caactgccct ttccggaacc agcccatgtt ttaaattgca aatgtgagta
     9541 accagtccat atccagatgc tgcctccaga tcacattttt ctttgatctc ctggaccaga
     9601 gaacactgcc ctttttcaca aagacttcct ctgccatcct gccatgtata caaaggactg
     9661 acacatgtac ccgtctgatc tatgtatacg atcccatgca tctgtccggt aagaccgatc
     9721 cgctctgtct gcgggtattt atctaaaaga tcatccaaca gtttttcagc ctttttaacg
     9781 atcgccccgg catcctggat cttttcccat tcattggcag ttggcataaa gccgccattt
     9841 tctatggttt ttgcttccag taattttacc gttttttcgt tttcatactc taaaactgca
     9901 ccgctgatgg tcgttgtccc aatatcgatc cctattatct gcatcttctg ttccccatct
     9961 gtttcgtggt cataaaaatg tacggaacac tatttattct acccagaaca ctctattttc
    10021 ttttctcggc gctgcttttg gaagatcgcc atctgcatag ccaactacac agtggccgat
    10081 gccttcccat tcgccttcta cacccaggtc ctttaacaac tgcttatact ccggcagttc
    10141 aaattcttct ttcgccctgt ggatccagat gcttccaaga tctaaggctt tcgcttccag
    10201 catcatattg cccattacaa gacttccgtc atacacatgg gtcggacagt ttttgtcagc
    10261 cagcacaaca aggaccgccg gtgcaccata aaaaggatca aatccttcct cccagcctcc
    10321 gatcctgcgg ttcatctctg acagtttatc cctaagttcc ttattggtaa cagcaacaat
    10381 gatcgctgcc tgctttccca taccgcttgc tgcatacaga ccagcctcta tgatctggtt
    10441 taaatcctct ttagatggca tatccggttt aaacttgcgg atgctgcggc gttctttcat
    10501 aacattcata atctcgttca taacaattcc tccatcattt ctgatatact ttctgtttta
    10561 tataatatta tctcacaaga ctttattttt tcaatttatt tatttgataa atcatcgatt
    10621 ttttctttta atgactgtat ctcttccaaa ataagcaccg tacatcccac tacaaaccct
    10681 gctaaaagga taagacctcc gtaaatggga tataaaaagg actcagctac acctccgccg
    10741 actacattgg aaagtaagtt ccaaaatcct gcgcctggga aaatcattaa ggcaaacatt
    10801 gcaactattg ctattataac tttactcatt gtatatttcc ctccttaaaa gcatctactg
    10861 cctcttttat tccatctaaa tccgttccac cgccataatc cagcaccact aaccgccttt
    10921 tatcttttaa tatcacttca tccactgccg gttctgcctc atatgcatcc tcttgaggat
    10981 cgtttccttc ttgaccaccg gatcttatcc cgtctttgcg gaccatatac cggtaccagg
    11041 acacttccat atcctctgga agcgctccga tctccgccgg aatctcctgc ctggtacaat
    11101 cccacagttt tgtcatatcc ttgggataac atttgctggt tttgttgatg atccagggaa
    11161 taggacttgt atacacggta acgcctatag gtctgtcaat gctttttcca ctgggatcac
    11221 cttcttcata acttgccaat atcgtctgtt ttctcccggt gtccggttcc tcttcgctaa
    11281 aaaaatgcgt tgcagcccca ttgcacaatg gaataaggat catacaggca accccaactc
    11341 caaccagata ggtcatccag tcatctctat tacttccact tccattttcc cttacccatt
    11401 ttcttacgaa aagggcgatc agtacaatga gggtggagga aagtataaag ctgatcagag
    11461 cctccatgga agaaagaaat gccagcgcta taactcccag tatcaggatc ccatctccca
    11521 taaaagtata ctttttacat ttcagggtcc gtttaagatc ctgttccgta agaggctctc
    11581 ccttctttat ggccgccata tttccatgac tccagcgaaa cagccgtata tacgtcgctg
    11641 cccaggaaat gatcatacct gtcagcagca gagtcaaaag aagctgcatg ggatctgcaa
    11701 acagtttccc tggattttgc agatactgat acacagccca tatttctaat acaaccaaga
    11761 taggatagaa aaatttcacc gaccagttcc aggaaagggc tttctggatc tgaaactgtc
    11821 tctctttctc catatcgccc cttcgttttg ctgcatcatc tgttgtataa aatatatgcc
    11881 agtagatgct gttgcactga aaaatccagc ctgcttcctc gcactgctcc cgcaactgct
    11941 ctgctttcca gtcatctgcg cctataaggg aagagctttc cggtaccaga agggcggcat
    12001 atcttcgttt ttccggctgt gccctgtaaa atctcatcac agcgccacca acgctttcta
    12061 aaaaccagcc ctggagtgcc attttctcca gatattgctc aaatgcatct gcctcacaca
    12121 aattaaaagt aaaccagacc cgtttctttt ccttcatgtc cccatcccct ttccgcaaaa
    12181 cagtcctgtt ttctcaatgt tctatctatg atcattatag ataacgatcc tgttttctga
    12241 caagaaagag gaacttacta ttttcttaca tattacatgt gtatcttaaa tccattgatc
    12301 tcctcgatca gcttacttac ggctgtggaa taggggatat ttcttctcca ggcgatcaca
    12361 gtgctggttt tgatctcagg atacagtttt ttctggacca gaaggtccga tctccagtat
    12421 ctgccttatc tgcttctctg gaataccgca tttcaagcca taggcacaca atgccatgat
    12481 tgagaagtat cgtccaccaa ccttgact
//
LOCUS       DBMDUA010000072        12302 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2649_length_12302_cov_2.514466, whole genome shotgun sequence.
ACCESSION   DBMDUA010000072 DBMDUA010000000
VERSION     DBMDUA010000072.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 12302)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12302)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12302
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2649_length_12302_cov_2.514466"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="ACZPCL_07120"
     CDS             complement(<1..408)
                     /locus_tag="ACZPCL_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1162270.1"
                     /translation="MNIRQSQEELELVTLSPYASFSAKTKGRDRQEPLCDIRPEYQRD
                     RDRILHCKAFRRMKHKTQVFLAPEGDHYRTRLTHTLEVSQIARTIAKALRLNESLTEA
                     IALGHDLGHTPFGHSGEAILNVICEDGFTHYEQS"
     gene            complement(625..1929)
                     /locus_tag="ACZPCL_07125"
     CDS             complement(625..1929)
                     /locus_tag="ACZPCL_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR1162271.1"
                     /translation="MSIAVQVALVMVVLLVLLLAAGVPIGVGIGLSSAVSMICMLPSN
                     VAFATSAQRIFAGSNSFSLIAIPFFILAGNIMNNGGIALRLVNCAKVIGGRMPGALAQ
                     SNVIANMLFGAISGSGAAAAAAIGSTMGPLEEAEGYDKNYSAAVNVASAPVGMLIPPS
                     NTMIVYSTVAGSVSISALFMAGYVPGLIWGLGVIILATITAKKLGYRGEAKLTASIVL
                     KTFWQAIPSLLMIVIVIGGILCGVFTATEGSAVAVVYATVLSLIYGSIKIKDIPRIVL
                     ESAKITGTITFMIGLSSIMSWAMAFTGIPDMVAAAVLGITSNKIIIFLLINIILLFVG
                     TFMDPTPAILIFTPIFLPICTSLGMNAVQFGIVLCYNLSVGTITPPVGTILFTGCKVG
                     GVTIESVISHLLPYFVMIIIGLLLVTYVPVLSLGLPSLLGLL"
     gene            complement(1933..2412)
                     /locus_tag="ACZPCL_07130"
     CDS             complement(1933..2412)
                     /locus_tag="ACZPCL_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR1162272.1"
                     /translation="MKTLKHGLDRFLAFVCIVDFIVMVLLTVYQVVVRYVFKSPSSVS
                     EVLTRYCFVWLILLSATYVFGQRDHICITYLKDKLNGSTKRLLDAAIEVVIILFSALI
                     LVYGGSVITTMNMLQYDSILKIPTGTIYSIIPVCGVLIIFYSIYNLTVELNKKEGGE"
     gene            complement(2412..3416)
                     /locus_tag="ACZPCL_07135"
     CDS             complement(2412..3416)
                     /locus_tag="ACZPCL_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR1162273.1"
                     /translation="MFKKLAMAVGVICLMGMLTACGRTSEQKVTEIRVAFNQNENHPQ
                     YRAMKAFGEKFEKETNGRYHVTIYPNGVLGEQGAMAEFIRTGALQMAIVPCSVPEGYD
                     ADFAIVGAPYLYDNIDHLEKATLNGVFDDLFASTEKYKFRVLTVYTAGERNVYAKKPI
                     NSADDLKGMIIRVNDSPTYVEMAKLMGGNGSVMAQSEVYTALQQGVIDAAENSELVYR
                     DFKHYEVAPYYAYTRHIVHPDVVLASTAFLNSLSDEDREIFDRLIKESTEEEFETFKQ
                     DIEVAKKDAEEAGTQFCYPDTNELRERCMPLLNKIANQSDMTKQIYDAVVALREEDNK
                     "
     gene            complement(3662..4126)
                     /locus_tag="ACZPCL_07140"
     CDS             complement(3662..4126)
                     /locus_tag="ACZPCL_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NanQ anomerase/TabA/YiaL family protein"
                     /protein_id="HIR1162274.1"
                     /translation="MIFSSIKNGDSFEKYPAAIQTALEYLKSHDFASMEPGVYEIQGK
                     DIYAQVFDAMTEDVADRRPESHEKYLDVQFLVSGRERLGFTKNTGNYKVAEHIKERDL
                     IFYESVEDEGYIESRPGCFCVFFPSDIHRPQVISGEAMNLRKVVVKVSVALL"
     gene            complement(4174..5088)
                     /locus_tag="ACZPCL_07145"
     CDS             complement(4174..5088)
                     /locus_tag="ACZPCL_07145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009445621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR1162275.1"
                     /translation="MRIAVIGAGAMGSIYGGHLSQHNDVLLVDTNEKVVEQVANNGLL
                     IDEDGTTHTYHPGAVSNTKGQEPADLVILFVKALFSRAALEGNRGLIGPNTRLMTLQN
                     GAGHEDLLKEFVDEDHIVIGTTEDNGAVLGMGHVRRGGTGVTNVGMLVEDKEGFLPQL
                     KEAFDQCGFNVKIHENIQTLIWDKLFTNVSLSALTGVLQVDMGYIAANEYAWDLCCQL
                     IHETVKTAAAAGLNFDEEKVREKVKNTSINNPNGCTSIRADLRDGRRTEVDTISGAVV
                     RAAHKYGVEVPGHECIVRLVHAMEGRNK"
     gene            complement(5204..5890)
                     /locus_tag="ACZPCL_07150"
     CDS             complement(5204..5890)
                     /locus_tag="ACZPCL_07150"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR1162276.1"
                     /translation="MCVYEMGNLRIVDLSKILDPKTESRRCHLFRFNTGGAIPDFHTN
                     MDLMSHLGTHCECPYHHDDNWPSVAELPLTTFLGRAVYVDFKETVAKRGHITAADLDR
                     EAGKVREGDIVIIDSSYKLYPFTPDTNTDKDQRLLVGAESAEWFKAHKVKAVGFGDGV
                     SIENCNEDVKPFHDILMAENIVFLEVLRNLEQLKKDVFFMSYSPLPILGLDSSPVRAY
                     AIEGLAEFSE"
     gene            6268..6939
                     /locus_tag="ACZPCL_07155"
     CDS             6268..6939
                     /locus_tag="ACZPCL_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1162277.1"
                     /translation="MKEKSFLQMQAYDSIKNGILNGELEPGQLYSETKLSAKIGISRT
                     PMREALQCLSQDGYITIIPSKGFMIRQLNEKDMKDSIQIRCAIEGFCTTVIASQTDTK
                     EGKELLRSLKMILDNMEEALNSDALEDFIYYDHQFHLLIVNYLHNDEFNQIFQRLLYL
                     IHLTSQDALSTEGRIQGTVDEHLAYYNALKEGDGQKAYNIMIEHLMMPLKVHEKVREK
                     KSEKK"
     gene            complement(7032..7499)
                     /gene="ribH"
                     /locus_tag="ACZPCL_07160"
     CDS             complement(7032..7499)
                     /gene="ribH"
                     /locus_tag="ACZPCL_07160"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR1162278.1"
                     /translation="MRTLEGKVVAREIKVGIVAARFNEFIVSKLVAGARDGLLRHDVK
                     DEDIDLAWVPGAFEIPLIASKMAKSGKYDAVICLGAVIRGATSHYDYVCSEVSKGIAS
                     VSLTSDIPVMFGVLTTDNIEQAIERAGTKAGNKGYDCALGAIEMVNLVRQLEN"
     gene            complement(7539..8756)
                     /locus_tag="ACZPCL_07165"
     CDS             complement(7539..8756)
                     /locus_tag="ACZPCL_07165"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HIR1162279.1"
                     /translation="MDQRKNTENGFNTVEEALEDLRQGKLILVTDDPDRENEGDFICA
                     AEFATTENINFMAVHGKGLICMPMSEAYVEKLQLPQMVTKNTDNHETAFTVSIDCVDT
                     TTGISAAERSITAMRCVAEDAKPEDFRRPGHMFPLLAKKNGVLERNGHTEATVDLCRL
                     AGLKECGLCCEIMKEDGTMMRTPQLKELAKEWNIKFITIKDLQEYRKVHEQLVERVTI
                     TKMPTKYGVFKAYGYINRLNGEHHVALVKGDIGNGEDVLCRVHSECLTGDVFGSLRCD
                     CGQQFAAAMTQIEKEGRGILLYMRQEGRGIGLINKLRAYELQEQGMDTLEANLALGFK
                     GDEREYYIGAQILRDLGVRSMRLLTNNPDKVYQLSAFGIKITERVPIQMQETEYDHFY
                     LQTKKIRMGHMLQ"
     gene            complement(8812..9483)
                     /locus_tag="ACZPCL_07170"
     CDS             complement(8812..9483)
                     /locus_tag="ACZPCL_07170"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR1162280.1"
                     /translation="MFTGIIEEKGTISKIMKNASQAVLTIKAQKIMEDIHKGDSIAVN
                     GICLTVTAFSKNEFQADVMHETLNRTALKMLKPGSSVNLERAMAAGGRFGGHMVSGHI
                     DGTGKIAEIKEDSLAIWYTIEAPPQILRYIVEKGSIAIDGISLTVAKVTQTTFSVSTI
                     PHTRSATTLGERRPGDLVNLENDIVGKYVEKFICEKNTHGAIADKNEKKTHGITREKL
                     MQYGY"
     gene            complement(9464..10552)
                     /gene="ribD"
                     /locus_tag="ACZPCL_07175"
     CDS             complement(9464..10552)
                     /gene="ribD"
                     /locus_tag="ACZPCL_07175"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR1162281.1"
                     /translation="MSVAIELAKKGYGYTAPNPVVGAVIVKDGCIIGQGYHEKYGEPH
                     AERNALASCSQSPEGATIYVTLEPCCHHGKQPPCTDAIIEAGISRVVTGSGDPNPLVG
                     GKGIRILKDHGIQVTEHVLKEECDGLNPVFFHYIQTGRPYVTMKYAMTMDGKIAAYTG
                     ASRWVTGEEARHHVHEQRKKNTGIMVGIGTVLADDPMLNCRIEGGRDPIRIICDTHLK
                     TPVTSKIVKTAKDIPTIIACCVSDEELQRPYKEAGCKILLTEKKMGHIDLQHLMELLG
                     KEKIDSVLLEGGGTLNWAALEAGIVQKVQAYIAPKLFGGTTAKTPVEGRGVEEPDQAF
                     LIKHKKVRVLGEDLLIEGEVERNVYGDH"
     regulatory      complement(10854..10968)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(10995..12020)
                     /gene="mmuM"
                     /locus_tag="ACZPCL_07180"
     CDS             complement(10995..12020)
                     /gene="mmuM"
                     /locus_tag="ACZPCL_07180"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HIR1162282.1"
                     /translation="MTFKRTTLEETLAVSPLLVLDGAMSTPLENMGIDLNTKLWTAKV
                     LADQPEMVKQVHKNYFKAGADCGITCSYQATVPGLMDIGYTKEQAEELIAKSVEIFLE
                     AREEWWQEEGEKAGHTWPLCLASVGPYGAYLADGSEYRGHYEVSEETLYEFHKHRMEL
                     LWEAGADLLLLETQPSLREALVEAKIAEEMGADYWISFSCGDDKHINEGTKIAECARA
                     FSRDPHKYPHLKMIGVNCTKPAYIVSLIREFRTATDLPIAVYPNSGEEYDPVTKTWHG
                     TNDKRTFYDYALSYFQNGATAVGGCCTTVESHIRQVTQARRDFEKAGIILKMRKMVKK
                     PLAISKK"
BASE COUNT         3206 a         2965 c         2377 g         3754 t
ORIGIN      
        1 gctttgttca tagtgggtaa aaccatcctc gcagatcaca tttaaaatag cttccccgga
       61 atgtccaaag ggcgtatgcc ccagatcatg ccctaatgca atagcttcgg tgagactttc
      121 atttaaccgc agggctttcg caatggtcct tgcgatctgt gacacttcta atgtatgggt
      181 aagtcttgtg cggtaatgat ctccctcagg cgcaagaaat acctgggtct tatgtttcat
      241 acggcgaaat gccttacaat gcagaatacg gtccctgtcc cgctggtatt ccggccggat
      301 atcacagaga ggttcctgcc tgtctctgcc ctttgttttt gcagaaaaag aggcatacgg
      361 gcttaaagtt acaagttcca gttcttcctg agattgtctg atgttcaata agtcaccccg
      421 cttttgtggc gaaaaggcac tttgcagtcc tttttcattt tcgctatgta tatgtgtatg
      481 ctggtcatta taattttatg acaactaatt aatttatcgt actattttta tagtatagca
      541 catttaaaaa acagacgcca ccggtaaaat tccgatgacg cctgtttcat tattctttat
      601 gctattttgc ttacgcacaa tttcctacag taagcctaac agtgacggca gtcccagtga
      661 aagtacagga acataggtca caagcagcag accgatgatg atcattacaa agtatggcag
      721 caggtggctg atgacacttt caatagtaac gccgcctacc ttacagcctg taaacaggat
      781 ggttcctaca ggaggtgtaa tggtacctac ggacaggttg tagcaaagta cgataccaaa
      841 ctgaactgca ttcattccaa ggcttgtgca gatcggcagg aagattggag tgaagatcag
      901 gattgctggt gtcgggtcca tgaaagtacc tacaaacagc aggatgatat taattaacag
      961 gaagatgatg atcttattgc ttgtaatacc aagaacagct gcagctacca tatccggaat
     1021 tccggtaaat gccattgccc aggacatgat agaagaaagt ccgatcatga aagtaatggt
     1081 acctgtgatc tttgcggact ccagtacgat ccttggaata tccttaattt taatagaacc
     1141 ataaattaag gaaagaacgg ttgcataaac aactgctact gcggaacctt cggttgcggt
     1201 aaatacaccg caaagaatac cgccgattac aataacgatc attaaaaggc ttgggatcgc
     1261 ctgccagaaa gtctttaata caatgctggc tgtcagcttt gcttcgcctc tgtagcctaa
     1321 tttctttgct gtgatggttg ccaggatgat aacgcctaag ccccagatga gaccaggaac
     1381 ataaccagcc ataaacagtg cggaaataga tacacttcct gcaaccgtag aataaacgat
     1441 catagtgttg cttgggggaa tcagcatacc aacaggtgct gatgcaacgt ttactgctgc
     1501 ggaatagttc ttatcatagc cttcggcctc ttccagcggt cccatggtgc ttccgatggc
     1561 tgcagcagct gctgcaccgg aaccactgat cgcaccgaaa agcatgtttg cgattacatt
     1621 ggactgtgct aatgcaccag gcatacggcc gccgatgacc tttgcacagt ttacaagtct
     1681 taatgcgata ccaccgttat tcataatgtt tcctgcaaga atgaaaaacg ggatcgcgat
     1741 cagtgagaat gagttggaac ctgcgaaaat tctctgtgcg gatgttgcaa acgctacatt
     1801 ggatggcagc atgcagatca tggaaactgc ggaagaaagt ccgattccca caccgatggg
     1861 cacacctgct gccagtaata aaaccagaag aacgaccatt accagggcaa cctgaactgc
     1921 gatagacata acttactctc ctccttcttt cttgttcagt tctacagtga gattatagat
     1981 gctgtagaaa atgatcagta cgccacatac aggaatgata ctgtaaatgg ttcctgtagg
     2041 gatcttaagg atagagtcat actgcagcat gttcatggtt gtgataacgg aaccaccata
     2101 aaccaggatc agcgcagaga agagaataat aactacttcg atcgcagcat ctaacagtct
     2161 cttggtgctg ccattcagtt tatcctttaa ataagtaatg cagatatgat cacgctgtcc
     2221 aaacacatag gttgcggaca gaaggatcag ccatacaaag cagtaacggg tcagaacttc
     2281 gctgacactg cttgggcttt tgaacacata gcggactact acctggtata cggtaagaag
     2341 gaccataacg ataaaatcaa cgatacagac gaaagctaaa aagcggtcta atccatgttt
     2401 taatgttttc attatttatt gtcctcctct cttaaagcta ctactgcgtc atagatctgc
     2461 tttgtcatgt cagactggtt tgcgatctta tttaacagag gcatgcagcg ctcacgcagt
     2521 tcgttggtat ccggatagca gaactgggtt ccggcttctt cggcatcttt ctttgctact
     2581 tcaatatcct gtttaaatgt ttcaaattct tcttcagtgg attccttgat cagtctgtcg
     2641 aagatctctc tgtcttcatc acttaaggaa tttaagaatg cggtacttgc aagtactacg
     2701 tcaggatgta caatgtgacg ggtgtaagca tagtaaggag ctacctcata atgcttgaag
     2761 tcacggtaaa ccagctcact gttctcagct gcatcaataa cgccctgctg cagtgctgtg
     2821 taaacctcag actgtgccat aacgcttccg ttaccgccca tcagttttgc catttctaca
     2881 taggtcgggc tgtcatttac acgaatgatc atacctttaa gatcgtctgc agaattgatc
     2941 ggtttctttg catatacatt tctctcgcct gctgtataaa cagtcagtac acggaatttg
     3001 tatttttcag tagatgcaaa cagatcatca aataccccgt ttaatgttgc tttttcaaga
     3061 tgatcgatat tatcatacag ataaggtgca cctacgatcg caaagtcagc gtcatatcct
     3121 tccggaacgg aacatggcac aattgccatc tgcagcgctc ctgtacggat aaattcagcc
     3181 attgctccct gctctcctaa taccccgtta ggataaatgg ttacatgata acgtccgttt
     3241 gtttccttct caaatttttc cccaaatgct ttcatggcac gatactgcgg atggttctcg
     3301 ttctggttga aagctacgcg aatctctgtc accttctgtt cggaagtacg tccacaggct
     3361 gtaagcatac ccataaggca aataaccccc acagccattg ccaatttttt aaacataccg
     3421 gtaaatcctc ctactggttt tacaaattga tttttcaaat attttcttgc tgttaacttg
     3481 cgttaacgca ttttttaaca ctttttttac ggatttttcc aatattatag cagttcagca
     3541 tattggcccg aaaatcgtct ttcaggccag tattacatgc tgagctgcta agtttactga
     3601 tctataaaat ttttatatac acaattatcc tgtacgcagc gacactgccc ccgtaatata
     3661 attacagcag tgctacgctt accttaacta caaccttgcg caggttcata gcttcgccgg
     3721 agataacctg tggacgatgg atatcggatg ggaagaatac acagaaacat cccggacggg
     3781 actcaatgta tccttcatct tctacagact catagaagat caggtctctt tccttaatgt
     3841 gctctgctac tttgtagttt ccggtgttct tggtaaatcc aagacgctct cttccggata
     3901 ctaagaactg tacatccaga tatttctcat gagactccgg acgtctgtca gcaacatctt
     3961 ctgtcatggc atcaaatacc tgagcataga tgtcttttcc ctggatctca tatacaccag
     4021 gttccataga tgcgaaatcg tggctcttta agtactctaa tgcagtctgg atcgctgcag
     4081 ggtacttttc aaaagaatca ccattcttga tagatgaaaa gatcattttt tctctccctt
     4141 tattttattt aaagccttta taaataaaag cttttatttg tttctgcctt ccattgcatg
     4201 tacaagacgt acaatgcact catggcccgg aacttccaca ccatacttgt gtgctgcacg
     4261 tactacagcg ccgctgatgg tatcaacttc tgttcttctg ccatcacgca ggtctgcacg
     4321 gatagaagta cagccgtttg gattgttgat ggaagtattt ttaacttttt cacgaacttt
     4381 ttcttcatcg aaattcagtc cggctgctgc tgctgtcttt actgtctcat ggatcagctg
     4441 gcagcacaga tcccacgcat actcgttagc tgcaatgtag cccatgtcta cctggagaac
     4501 acctgtaagg gcacttaagg aaacattggt aaacagcttg tcccagatca gagtctggat
     4561 gttttcatgg atctttacat taaaaccaca ctggtcaaat gcttctttta actgaggaag
     4621 gaaaccttcc ttgtcttcta ccagcatacc aacattggta acaccggttc cgccgcggcg
     4681 aacatgtccc attccaagaa cagcaccatt atcttcggtt gtaccgataa cgatatggtc
     4741 ttcatcaacg aattccttta acagatcttc gtgtcccgca ccgttctgta aagtcatcag
     4801 gcgggtgttt ggtccgatca gaccacggtt accctctaat gctgccctgg agaacagagc
     4861 ctttacaaag aggatcacta aatctgccgg ttcctgtcct ttcgtgttgc tgactgcacc
     4921 tggatgataa gtatgagtgg ttccgtcttc atcgatcaga agaccgttgt ttgcaacctg
     4981 ctctactact ttttcattgg tatctactaa aagaacatca ttatgctggg aaagatgtcc
     5041 accatagata gaacccattg ctcctgcacc gatcactgca atacgcattt tactttctcc
     5101 ttttcccatt cttacgttta acgtttacac tgtgccttaa agcagattgc gccctgcata
     5161 aactcttctt atgcgtgggc gcagtctttt aaaagtccgg ggtttattcg gagaactcag
     5221 caagtccttc aattgcatat gcacgaacag ggctggaatc cagtccaagg attggaagtg
     5281 gggaatagct catgaagaat acatccttct taagctgctc taagtttctt aaaacttcca
     5341 ggaatacaat gttctctgcc attaagatgt catggaaagg cttaacatct tcgttgcagt
     5401 tttcgatgga aacaccatca ccaaagccaa cagcttttac cttgtgagcc ttgaaccact
     5461 cagcgctctc agcaccaact aacagacgct gatccttgtc agtgttggta tcaggtgtaa
     5521 atggatacag cttgtaggaa gagtcgatga taacgatatc tccttctctt acctttccag
     5581 cttcacggtc taagtcagca gcggtgatat gtccacgctt agcaacagtc tccttgaagt
     5641 ctacgtatac tgcacggcct aagaaagtgg ttaaaggcag ctcagctaca gatggccagt
     5701 tatcatcatg atgataaggg cactcacagt gggttcccag atggctcatc agatccatgt
     5761 tggtgtggaa atccgggata gcaccgccgg tattgaagcg gaacagatgg catcttctgc
     5821 tctctgtctt tggatccagg atcttggaaa gatcaacaat tcttaagtta cccatttcat
     5881 aaacacacat tttaggttcc tccttaaaat tggtttttat tttctttgtt ggcttttttc
     5941 tcgcccatct gtataccagt ataccggtat gtcggtatac tgtcaagatt ttcaacgaaa
     6001 taaaagtcaa caaattttta acgatgtttt tgtgcatttc tgataatgca ctttcttata
     6061 cccttgtcat atatatacaa aatagttact ttcagtttac aataagttac ttttctatac
     6121 aagacttgat ttgaaaatat cccctatccg gcaaaaaaat ggggaaaata ataggaaaat
     6181 caatggtttt tcttgacaga cataacactt tagccttaag attgtataca gaatttgatt
     6241 taaatataaa ggatatggat acaagaaatg aaagaaaaaa gcttcctgca gatgcaggcc
     6301 tacgattcca ttaaaaacgg cattttaaac ggcgaactag agccgggaca gctctactcc
     6361 gaaacaaaac tgtccgcaaa aataggaatt tcccgaaccc ctatgcgtga agccctccag
     6421 tgtttaagcc aggacggcta tatcaccatt ataccaagca aaggctttat gatccgtcag
     6481 ttaaatgaaa aggatatgaa agacagtatc cagatccgct gcgctattga aggcttctgt
     6541 accaccgtga tcgcatcaca gacagatacc aaagaaggaa aagagttgct gcgcagttta
     6601 aaaatgatcc tggataacat ggaagaagca ttaaattctg acgctttgga agatttcatt
     6661 tattatgacc atcagttcca tctgcttatc gtcaactatc tccacaatga tgaattcaac
     6721 cagatcttcc agcgcctgct gtatctgatc cacttaacct ctcaggatgc cctttccaca
     6781 gaaggccgta tccagggaac tgtggatgaa catctggctt attataacgc attgaaagaa
     6841 ggggatggcc agaaagctta caatatcatg atcgagcatc tgatgatgcc tcttaaggtg
     6901 catgagaaag tacgggaaaa gaaaagtgaa aagaaataat atacgccaaa agccgcctgg
     6961 atgggcggct ttttttgcgt tcacgattgt tattgccaat ctcatgatcc atattttgca
     7021 ttttctgctt ttcaattttc cagctgacgc accaggttta ccatttcaat agcaccaagg
     7081 gcacagtcat agcctttatt tcctgctttt gttcctgcac gctcaatggc ctgttcaata
     7141 ttatccgtgg tcagaactcc aaacatgacc ggaatatcgc ttgttaaaga cacactggca
     7201 atgcctttag aaacctcact gcacacgtaa tcataatggc tggttgctcc tcttataaca
     7261 gctcccaggc agatcactgc atcatatttt ccgctttttg ccattttaga tgcgatcaac
     7321 gggatctcaa aagctcctgg aacccatgca agatcaatgt cctcatcttt tacatcatgg
     7381 cgcagcaggc cgtctctggc acctgccact aattttgata ctataaattc attaaatctg
     7441 gctgctacga ttcctacttt gatctctctt gctactactt ttccttctaa tgtacgcatg
     7501 atttttgttt cctttcgtat atgtatgtta ttttaatatt actgcagcat atgacccata
     7561 cggatcttct tggtctgcag ataaaaatga tcatattctg tttcctgcat ctggatcggt
     7621 acccgctctg tgatcttaat gccaaaggca gacagctgat acactttatc cgggttattg
     7681 gtaagtaaac gcatgcttct tactcccagg tctcttaata tctgggcgcc gatataatat
     7741 tctctttcat ctcccttaaa accaagagca agattggctt ctaaggtatc cattccctgt
     7801 tcctgcagtt cataggctct taatttattg atcaggccga tccctctgcc ttcctggcgc
     7861 atatacaaca ggatgcctct gccctctttt tcaatctgtg tcatagctgc agcaaactgc
     7921 tggccgcaat cgcagcgcag ggaaccgaac acatctccag taaggcattc tgaatggact
     7981 ctgcaaagca catcttcccc gtttcctata tcacctttta ccaatgccac gtgatgttct
     8041 ccgttcagtc ggtttatata tccataagct ttaaacacgc catattttgt aggcattttc
     8101 gttatagtca cccgctccac cagctgctcg tggacttttc tgtactcctg aagatctttg
     8161 atagtgataa atttgatatt ccattctttc gccagttcct ttaactgcgg ggtgcgcatc
     8221 atggttccgt cttctttcat gatctcgcag cagaggccac attcctttaa accagccagg
     8281 cggcacagat caacggttgc ttctgtatgt ccattacgct ctaaaacacc atttttcttt
     8341 gcaagaagag ggaacatatg acccggacgt ctaaaatctt ccggttttgc atcttctgct
     8401 acgcagcgca tggcagtgat agaacgctct gctgcagaga taccggttgt agtatcaaca
     8461 cagtcaatgg atacggtaaa cgcagtttca tggttatctg tattctttgt gaccatctga
     8521 ggaagctgca atttttccac ataagcttct gacattggca tacagatcag gccttttccg
     8581 tggactgcca tgaaattaat gttttctgtg gttgcaaatt ctgccgcaca gataaagtcc
     8641 ccttcgtttt ctctgtccgg atcatctgta acaaggatca gttttccctg tcttaaatct
     8701 tccagtgctt cctctactgt attaaatcca ttttctgtat tcttcctctg atccatgata
     8761 tttctcctct cttcttctct acgtacttcg ggatttttta attcatatcc attaataccc
     8821 gtattgcatc agtttctctc tcgttattcc gtgagttttt ttctcatttt tatccgctat
     8881 tgctccgtga gtatttttct cacatatgaa cttttccaca tatttcccta caatatcatt
     8941 ttccagattg accagatctc ctggtctccg ctctcctaat gtagtagcac tccttgtatg
     9001 gggtatggta gaaacagaga aagttgtctg cgttaccttt gctactgtaa gactgattcc
     9061 gtcaatggca atcgaacctt tttctacaat atatcgaagg atctgtggcg gtgcttctat
     9121 ggtgtaccag atcgccagac tgtcttcttt tatctctgcg atttttccgg taccgtcaat
     9181 gtgaccggat accatgtgac ctccaaacct gcctcctgct gccatagccc gctccagatt
     9241 tacactgctt cccggcttta gcattttaag ggcagtgcgg tttaatgttt catgcattac
     9301 atctgcctga aattcatttt tagaaaatgc tgtaaccgta agacagatcc cgtttactgc
     9361 aatgctgtct cctttatgga tatcttccat gatcttctgc gcttttatag taaggactgc
     9421 ctgactggcg tttttcataa ttttactgat cgtgcccttt tcttcaatga tccccgtaaa
     9481 catttctctc cacctctcct tctatcagca ggtcctctcc cagaactctg acctttttat
     9541 gtttgatcag aaatgcctga tccggttctt ctacgcctct tccttctacg ggagtcttgg
     9601 ccgttgttcc cccaaaaagc tttggcgcta tatatgcctg tactttctga acgatccccg
     9661 cttctaatgc cgcccagtta agagtaccgc cgccctctaa cagaacgctg tctatcttct
     9721 ctttccccaa cagctccata agatgctgca gatcaatatg gcccattttc ttctctgtta
     9781 aaagtatttt gcagcccgcc tctttataag gcctttgcag ttcttcatct gatacacagc
     9841 aggcaatgat agtaggaata tcctttgcgg tttttacgat cttcgaggtc acaggcgtct
     9901 ttaaatgagt atcgcagata atacggatgg gatctctgcc tccttctatg cggcagttaa
     9961 gcataggatc atctgccagt accgtaccga tccccaccat gatcccagta tttttctttc
    10021 gctgttcatg tacatgatgc ctggcttctt ccccggtcac ccatctggaa gcaccggtat
    10081 aagcggcgat ctttccatcc atagtcatgg cgtatttcat ggtcacataa gggcggccag
    10141 tctggatgta atggaaaaat accggattta atccgtcgca ttcttccttc aacacatgtt
    10201 ctgttacctg tattccatga tcctttaaaa tccggattcc ttttccaccc accaatggat
    10261 tgggatcccc ggaaccggtc actacccggc tgatacctgc ttctatgatg gcatctgtgc
    10321 atggtggctg ctttccgtga tgacagcagg gttccaacgt tacatagatc gtggcgcctt
    10381 ctggagactg gctgcaggaa gccaacgcat tccgctccgc gtgaggttct ccatattttt
    10441 catggtatcc ctgaccaata atacagccat cttttacgat cactgcccca actaccggat
    10501 ttggcgctgt atagccgtac cccttcttcg ccagctcaat ggctacggac atatattttt
    10561 cgtcttcact gtaattctct gtttttttca aactttcgcc tcctcgtttt ctttcaggaa
    10621 cgcctaatcc gttgacacat ctctaatgca tcccgcaata tgcatggcat caaaaaacgc
    10681 cctggatcct atcgtattct ccagagcgtt ttgtaaaaaa tgggaaattc atgataattt
    10741 ttaaataact caaacactgc ctaaaaatca aaaagccctg aaacaattac tgtttcagag
    10801 ctgatgttca acaaatatct gatgctatat cttactatat catctatcct gttctctatc
    10861 ttctttcatc cagactgtac tgtcggcctc ggaatttcac cgaatcatac cttgcggctc
    10921 gtgggctata ccaccggtgg ggattcacac cccgccctga agatcttatt cacttgttag
    10981 ttgtaactat aatactattt ttttgaaatt gcaagaggtt ttttaaccat ttttctcatt
    11041 ttcagaataa tccccgcctt ttcaaaatcc cgtctggcct gggtcacctg ccggatatgg
    11101 ctttctactg tggtgcagca gccaccaaca gctgtagctc cattctggaa ataagataaa
    11161 gcatagtcat aaaaagtgcg cttatcattg gttccatgcc aggtctttgt gactgggtca
    11221 tattcttctc cgctgtttgg gtataccgca atgggcagat ctgtagcggt ccgaaattcc
    11281 cggatcagac tgactatata tgccggtttg gtgcaattaa ctcctatcat tttcagatga
    11341 ggatacttat ggggatcccg ggaaaatgct cttgcacact ctgcaatctt tgtgccctca
    11401 ttaatgtgtt tatcatcccc acaggagaaa ctgatccagt aatctgcccc catttcttcc
    11461 gcgattttag cctctacaag tgcctctctc aaagaaggct gtgtttcaag cagaagcaga
    11521 tctgcccctg cttcccacaa aagctccatt ctgtgcttat gaaattcata aagagtctct
    11581 tcggacactt cataatgccc ccgatattct gagccatctg ccagatatgc cccatatggg
    11641 cctacacttg ccaggcacag cggccaggta tggcctgctt tttctccttc ttcctgccac
    11701 cactcttctc tcgcttccaa aaagatctca acacttttgg cgatcagttc ttctgcctgt
    11761 tcttttgtat atccaatgtc cattagtccc ggaactgttg cctgatagct acaggttatc
    11821 ccgcagtccg cccctgcctt aaaataattt ttatggacct gtttcaccat ttccggctga
    11881 tcagctaaaa cttttgcagt ccacagtttt gtatttaagt ctatccccat attttcaaga
    11941 ggtgtagaca tggctccatc caatacaagc aacggtgata ctgccagtgt ttcctctaaa
    12001 gttgtgcgtt taaaagtcat gagcaaaatc tcccttctcc gttgtatctc ttccgatcaa
    12061 ttacacacat tatacactat ttttgttacc actacatcat cttataacga ttttaatcca
    12121 aaaagtaacc tctccatgta cacgggagtt tgacaccttt ccgaatgaaa aacagcgtat
    12181 aagccgcatg aatgcttata tttttttgtc aaatttgttg tttttatgta taggcttttt
    12241 cggctttttg tgctataatg gtattgaggt gaaaaaattg aaggtcaata catcaaaatc
    12301 aa
//
LOCUS       DBMDUA010000073        12283 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2655_length_12283_cov_2.728123, whole genome shotgun sequence.
ACCESSION   DBMDUA010000073 DBMDUA010000000
VERSION     DBMDUA010000073.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 12283)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12283)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12283
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2655_length_12283_cov_2.728123"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(97..702)
                     /locus_tag="ACZPCL_07185"
     CDS             complement(97..702)
                     /locus_tag="ACZPCL_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162283.1"
                     /translation="MKKKLLAAAVVLTAVLMLGGCEGLSAKNAKGPETEESCLYITKD
                     GNLQWVSVETYEGGSYSKEELLDFASEKIKEYNEANGTESSKEPVTLVSGSLEKGKAV
                     LITSYDSGESLVDFASEIGDDTMPFTEIKTGTPEELSEDLKAAGVDYEAALATGSFAV
                     VLQGEGMVQTEADLLDADGGCDVHDGHFIKTGSGTSVIVTK"
     gene            complement(780..1454)
                     /gene="recO"
                     /locus_tag="ACZPCL_07190"
     CDS             complement(780..1454)
                     /gene="recO"
                     /locus_tag="ACZPCL_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR1162284.1"
                     /translation="MRDVLSVTGMVLLSAPSGDYDRRLVLLTREKGKITAFAHGVRRP
                     GSSLMAASRPFSFGTFQVYEGRSAYNLQSAQITNYFDELSADMEGACYGAYFLEVASW
                     LSDENMDGTELLNLLYVSLKALSKEALPNPLVRRIFELRAMVINGVYTQEPEGRVSES
                     CVYAWQYVIATPLGSLYTFTLKEDVLREFSREVQHAMDEYIHHEFKSLEILEVLSGGK
                     TARPVS"
     gene            complement(1707..2618)
                     /gene="era"
                     /locus_tag="ACZPCL_07195"
     CDS             complement(1707..2618)
                     /gene="era"
                     /locus_tag="ACZPCL_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR1162285.1"
                     /translation="MEDNKQKKSGFVTLIGRPNVGKSTLMNHLIGQKIAITSDKPQTT
                     RNRIQTVYTDDRGQIVFLDTPGIHKAKNKLGEYMVNVAEHTLKEVDVVLWLVEPTTFI
                     GAGERHIAEQLSKVNTPVILVINKIDTIKVQEEILTFIAAYKDVCDFAEIVPLSALKD
                     KNTDVLLEQIFKYLPYGPQFYDEDTVTDQPMRQIAAELIREKALRLLDDEIPHGIAVV
                     IEQMKERPNGIIDIEASIVCERESHKGIIIGKGGSMLKKIGTVARREIENMMETKVNL
                     QLWVKVRKEWRDSELYMKNYGYNQKDV"
     gene            complement(2668..5616)
                     /locus_tag="ACZPCL_07200"
     CDS             complement(2668..5616)
                     /locus_tag="ACZPCL_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR1162286.1"
                     /translation="MNRQQMLEKVENLSAYEVKDRRFVEEMNSEGMVLEHRKSGARLF
                     LMSNEDDNKVFSIGFRTPPADSTGLPHILEHSVLEGSEKFPVKDPFVELVKGSLNTFL
                     NAMTYPDKTVYPVASCNDKDFQNLMDVYLDGVFHPSIYREPKIFKQEGWHYELPSEDA
                     PLTVNGVVYNEMKGAFSSPESVLERFTSSVLFPDTPYSNESGGDPAVIPNLSYEQFIQ
                     FHKDYYHPANSYIYLYGDMDMEEKLLWLDKEYLSAYDKKDFTLDSSIALQKPFTEPVE
                     KETTYSVTANEGTEDNTYLSLNTVVGTDIDPVLYVAFQILDYTLISAPGAPLKQALID
                     AHIGQDIMGGYENGILQPYFSVVAKNANKEQKGEFLSVVKGTLRKLADQGIDKKSLLA
                     GLNYYEFRYREADYGSAPKGLMYGLWSMDSWLYDADPMLHLQYQKTFDYLKKAAQEGY
                     FEQLIKDYLLDNPHEAVVIVSPEIDLTAREDAELAERLAKYKDSLSSEQVKALVKETA
                     ELKAYQEEPSTKEDLEKIPMLGREDIKRQSEPFSYKVKEEEKTTVVHSPMFTSGIAYI
                     KLLFDMNVIPKEDLPYASLLKSVLGYVDTENFTYRDLTSEIHLNSGGLDFYVSSWEDL
                     NEKGAFKGAFTAGIKVLYEKVGFAFDIIKEILTASKLDDEKRLGEILDEMRSRSRMRL
                     EGASHSAAVARACSYFSPVRAFDDMTGGIEYFHFLEKTADAYSKDEKFRKELVRKLKE
                     TAEKLFAADNLMVGYTADEEGYKALPEELKKFKAALSDKPSRRYAFEFVPELKNEGFK
                     TSSKVNYVARCGSFDAEAYTGALEVLKVIMNYEYLWQNLRVIGGAYGCMSNFGRSGTG
                     CLVSYRDPKLATTDKIYEGIPEYLKNFSIDERDMTKYVIGAMSSVDTPLSPAVKGSRN
                     LTAYLSGITDEMVQKKREQILDVTQEDIRALADILESILKTGAFCVIGNEQQIEADKN
                     MFKEVKELFH"
     gene            complement(5942..6781)
                     /locus_tag="ACZPCL_07205"
     CDS             complement(5942..6781)
                     /locus_tag="ACZPCL_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR1162287.1"
                     /translation="MADMILLGLAIICTAYFMVIVFYAGMGTSFAFIWLFFAALCVFL
                     VYGKWYYRKNMDRIPQWVPVSIVTTCIAGVVIMAALCIMVFMGAAASEERDLDYVIVL
                     GARVKEHTVSNSLKKRLDKAIEYAEKNPETYLVLSGGKGPDEPVSEAQAMYQYLVYNG
                     VRPSQLLLEEHSVSTVENLAYSKIIIDSHREVIRHQREERNRLTFQREPKGGYLIAAD
                     KPLDVGVLTSNFHVYRACMIAKKWGFENVYGISAQSDPVLFIHLCVRECASIVKDRIM
                     GNM"
     gene            complement(6824..>7947)
                     /locus_tag="ACZPCL_07210"
     CDS             complement(6824..>7947)
                     /locus_tag="ACZPCL_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR1162288.1"
                     /translation="RAKAHNFSLEDMKEGIAFAHAHDVKVYVTANILAHNQDLPGVEA
                     YFEELKEVGPDALIISDPGVFEIAKRVLPDTELHVSTQANNTNYGTYLFWNRMGAKRV
                     VSARELSLAEIKEIRSHIPDDMEIESFIHGAMCISYSGRCLLSNFFTGRDANQGACTH
                     PCRWKYSIVEETRPGEYMPVYENERGTYIFNSKDLCMIEHVPELIDAGIDSFKIEGRM
                     KTALYVATVARTYRKAIDDYLKDPKLYEANMEWYKEEIGKCTYREFTTGFYFGKPGSD
                     AQIYNSNTYVKNYTYLGTVEEADGKGRCRIEQKNKFSVGETIEIMKPDGRNLEVVVKS
                     ICDEDGNEQESAPHPKQILWVDLGADVDKYDILRKKEDN"
     assembly_gap    7948..7957
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8072..8719)
                     /locus_tag="ACZPCL_07215"
     CDS             complement(8072..8719)
                     /locus_tag="ACZPCL_07215"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR1162289.1"
                     /translation="MIVDNRITEYLHSLETSRGELLDTIEKKAIEDGVPIIRSETAAL
                     LRSLTAALRPENILEIGTAVGYSALQMCQVMPANCHITTIEKYEKRIPEAKENFRKAG
                     EESRITFLEGDADMWLKELKGKQFDLVFMDAAKGQYLNWLPLLLDLMPVGAVLISDNV
                     LQDGDVVQSRFAVQRRNRTIHSRMREYLYELKHMEEFETAVIPIGDGVTISTRIR"
     gene            complement(8706..9353)
                     /locus_tag="ACZPCL_07220"
     CDS             complement(8706..9353)
                     /locus_tag="ACZPCL_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR1162290.1"
                     /translation="MSSRTREINKITMAIVRISVKLMLLALLILLLYEAVIRGYAFGH
                     AVFYAEAVEAAPGHDITVVVKDGEDVSQAADELEKKGLIKNIYAFLFQSRFYDYDKIY
                     PGTYTFNTSMTSKEILQKLSEKPENEEDEKSGDKSGSTKTTQKSADQSTEKSESDQQK
                     RQSDLQTEEANAADPAKSTLSSGEAQADAHSYSGEDEEEGGWIEDVTEDGQNDRR"
     gene            complement(9371..11005)
                     /locus_tag="ACZPCL_07225"
     CDS             complement(9371..11005)
                     /locus_tag="ACZPCL_07225"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR1162291.1"
                     /translation="MIPLGGLEQIGMNITAFEYEDSIIVVDCGLAFPGDDMLGIDLVI
                     PDVSYLKQNIDKVKGFVITHGHEDHIGALPYILQQVNVPIYGTKLTIALIEHKLKEHN
                     LLKTTKRKVMKHGQSVNLGCFRVEFIKTNHSIQDASALAIFSPVGIVLHTGDFKIDYT
                     PVFGDPIDLQRFAELGKKGVLAMLADSTNATRPGFTMSERSVGKTFDTIFAEHTNRRI
                     IVATFASNVDRVQQVVNTAYKYGRKVVVEGRSMVTIIDIASELGYINIPEGTLIDIDQ
                     LKNYPPESTVLITTGSQGESMAALSRMAASIHKKVSIVPGDVVVLSSTPIPGNEKAVA
                     NVINELSAKGAKVICQDTHVSGHACQEDLKLIYSLVHPKFAIPVHGEFRHRMAQKELA
                     EYMGVQKGNALLVNSGDVVSLDEDSCQVTDHVACGAILVDGLGVGDVGNIVLRDRQNL
                     AQNGIIVVVLTLEKHSNQLLSGPDIVSRGFVYVRESEDLLEEAHNVVADAVADCLDRH
                     VSDWGKIKNIIKDSLSDFLWKRMKRNPMILPIIMEV"
     gene            complement(11385..11675)
                     /locus_tag="ACZPCL_07230"
     CDS             complement(11385..11675)
                     /locus_tag="ACZPCL_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIR1162292.1"
                     /translation="MDKPMNEREDCITMITDSGESVDFYVLEETRIHGKDYLLVTDAP
                     EDEDGECYILKDVSGENDSEALFEFVEDDRELDGVMGIFEELLKDADVELEK"
     gene            complement(11668..12120)
                     /gene="ruvX"
                     /locus_tag="ACZPCL_07235"
     CDS             complement(11668..12120)
                     /gene="ruvX"
                     /locus_tag="ACZPCL_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR1162293.1"
                     /translation="MRIMGLDYGSKTVGVAVCDPLGITAQAVETITRKDENKLRQTLA
                     RIEALAKEYGVEKFVLGYPKNMNNTVGDRAQKSEELKAALERRTGLPVELWDERLTTV
                     AAERVLMESGVRREHRKESVDQIAAAMILQGYLDHLSMTKKEDGENNG"
BASE COUNT         3016 a         3088 c         2327 g         3842 t
ORIGIN      
        1 ccattatacc aaacggaaca ggtttatgcc ttttttattg gctgaaatat tgaattttca
       61 aggttcaaca caccacatcg gtgtgggaag ttttagttac ttagttacga ttacacttgt
      121 tccggaacct gtctttataa aatgtccgtc atggacatca cagcctccgt ctgcatccag
      181 caagtcagcc tctgtctgga ccattccttc tccctgtaaa acaacagcaa aggaaccggt
      241 cgccaaagca gcttcatagt ccacacctgc agctttcaga tcttctgaaa gttcctccgg
      301 tgtgccggtt ttaatttccg taaaaggcat ggtatcatct cctatttcac tggcaaagtc
      361 cacaagactt tccccactgt cataagaggt gataagaact gcttttccct tttccagact
      421 tcctgaaacc agagttaccg gctcttttga gctttctgtc ccattggctt cattgtattc
      481 ctttatcttt tcagatgcaa agtctaaaag ttcctctttg gaatagctgc cgccttcata
      541 cgtttccacg ctgacccact gaaggttacc atcttttgta atataaagac agctttcttc
      601 tgtttccggt ccttttgcgt tttttgcaga taagccttcg cagcctccaa gcataagcac
      661 tgctgtcaga acaacagccg ccgcaagaag ttttttcttc attctcttcc taattccttt
      721 cagacatatg gcatattata tcctctgaat ggcataattt caagcccttt agctcatttt
      781 cagctaaccg gacgggccgt ttttcccccg gagagcactt ccaggatctc taaggactta
      841 aactcatggt gtatgtattc atccatggca tgctgcactt ccctggaaaa ttccctcaaa
      901 acatcttctt tcagtgtaaa tgtatacagg gaacctaagg gagtcgcgat cacatactgc
      961 cacgcataaa cgcaggactc cgacactctt ccttccggct cctgtgtata cacaccgtta
     1021 atgaccatgg cacgaagttc aaaaatgcgc cgtaccagcg gatttggcag agcctctttg
     1081 cttaatgcct ttaaagacac atacaaaaga tttaaaagct ccgttccgtc catattttca
     1141 tcggaaagcc aggaagccac ttccagaaaa taagccccat agcaggctcc ttccatatct
     1201 gctgacaact catcaaagta attggtgatc tgggcagact ggagattgta ggcagatctt
     1261 ccttcatata cctgaaaggt tccaaaagaa aaaggccggc ttgctgccat caggctgctt
     1321 cccggtcttc tcacaccatg agcaaaggcg gttatctttc ctttttcccg ggtcagaagc
     1381 accagccgtc tgtcatagtc accggagggc gcgcttaaaa ggaccattcc tgtaacactt
     1441 aaaacatccc gcatgctgcg ccctcctttc ttatttcatg tactctcgga atctttttgt
     1501 acttgatttt gcgagaagat tttttgtcag ttgtgcccaa atttctgagg cgtacgcggt
     1561 gcgtacgttg atgaaattcg ggtgcaaatg gcggaaaatc ggccgcaaag gcaagtgcag
     1621 aaaagactcc gagagtacat ttaatctgca agatctgtca tttaatgcac attcttacgt
     1681 ctatgcagtt tctattatat atacgtctat acgtctttct gattatatcc gtaattcttc
     1741 atgtaaagct cgctgtctct ccactctttt ctaaccttta cccagagttg gaggttgacc
     1801 ttggtctcca tcatgttttc aatctcacgg cgggctactg taccgatctt ctttaacatg
     1861 gagccgcctt ttccgatgat gatgcccttg tgggactcgc gctcgcagac aatacttgct
     1921 tcaatatcaa taataccgtt tggacgctct ttcatctgtt caatgacaac ggcaattcca
     1981 tgagggatct catcatctaa cagacgcagt gccttttcac ggatcagctc tgctgcgatc
     2041 tggcgcattg gctggtctgt tactgtatct tcatcataaa actgaggtcc ataaggcaga
     2101 tacttaaaga tctgttctaa aagcacatct gtatttttgt cttttaaagc ggataatggc
     2161 acgatctctg cgaaatcaca tacatcttta taagcagcga taaaggtcag gatctcttcc
     2221 tgcactttaa tcgtgtcaat cttgttgatg accaggatca caggtgtgtt cacttttgaa
     2281 agctgctctg caatatggcg ttctcctgca ccgataaagg tagtaggctc aaccagccat
     2341 aagaccacat caacttcctt taatgtgtgt tccgctacat tgaccatgta ctcgcccagc
     2401 ttgttttttg ccttgtggat acctggggta tcaaggaaaa cgatctgacc tctgtcgtca
     2461 gtgtaaacgg tctggatcct gtttctggtc gtctgaggct tatcagaagt aattgcgatc
     2521 ttctgaccga tcaggtggtt catcagtgtg gattttccaa catttggacg tccgatcagt
     2581 gttacaaaac ctgatttttt ctgtttgtta tcttccattc ttttctcctg tttaatatat
     2641 acgtactcat aaagtactgt ttatgaatta gtgaaacagt tctttcactt ctttgaacat
     2701 gtttttatct gcttcgatct gctgttcatt tccaatgaca cagaaagcac ctgtttttaa
     2761 gatggactcc aggatatctg ccaatgcgcg gatatcctcc tgggtcacat ccaggatctg
     2821 ttctctcttt ttctgtacca tttcatcggt aattccggat aaataggcag tcaggtttct
     2881 ggaacctttt actgccgggc ttaatggtgt atctacgctg ctcatggcac cgatcacata
     2941 ttttgtcata tccctctcat caatggagaa gttctttaaa tattccggga taccttcgta
     3001 gatcttatct gtagttgcaa gctttggatc tctgtaggat accagacagc cggttccgct
     3061 tcttccaaaa ttactcatac agccataggc gccgccgatc acgcgcaggt tctgccataa
     3121 atattcgtag ttcatgatga cctttaatac ttccagggca ccagtgtaag cttctgcatc
     3181 aaaagaaccg cagcgtgcca cataatttac cttggaagag gtcttaaagc cttcgttttt
     3241 cagctccggc acaaattcaa aagcatatct cctgcttggt ttatcactta atgcagcctt
     3301 gaatttcttt aattcttccg gaagagcctt gtagccttcc tcgtctgctg tataacctac
     3361 cataagatta tctgctgcaa acaatttttc tgctgtttcc ttcaactttc tgaccagttc
     3421 cttacggaat ttctcatcct tgctgtaggc atctgctgtt ttttccagga aatggaaata
     3481 ttcaattccg cctgtcatat cgtcaaaggc tcttaccggt gagaaatagg agcaggctct
     3541 tgcaactgct gcgctgtggg aagcaccttc caagcgcatt ctggatctgg aacgcatctc
     3601 atctaagatc tcacccagac gtttctcatc atctaattta gaagctgtaa ggatctcttt
     3661 gataatgtca aaagcaaaac ctactttttc ataaagaacc ttgatgcctg ctgtaaatgc
     3721 ccccttaaaa gcaccctttt cattcaggtc ttcccaggaa gaaacataaa agtccagtcc
     3781 gccgctgttt aaatggatct cactggtaag atcccggtag gtaaaatttt ctgtatccac
     3841 atagcctaaa acagacttta aaagggatgc ataaggcaga tcctctttcg ggatcacatt
     3901 catatcaaaa agcagtttga tataagcaat accggaagtg aacattggac tgtggacaac
     3961 cgtggttttc tcttcctctt ttactttgta ggaaaacggt tctgactggc gtttgatatc
     4021 ttctcttcca agcattggga tcttctccag atcctctttt gtggaaggtt cttcctggta
     4081 tgctttcagc tctgccgtct cttttaccag ggcttttacc tgttcactgc ttaaggagtc
     4141 tttatacttt gccagacgct ctgccagttc tgcatcttcc cttgcagtca ggtcaatttc
     4201 agggcttacg atcacaactg cctcatgagg gttatctaag agataatctt tgataagctg
     4261 ctcaaaatag ccttcctggg ctgctttttt cagataatca aaggtcttct gatactgtaa
     4321 gtgaagcatt ggatctgcat catacagcca gctgtccata gaccataaac cgtacatcag
     4381 tccctttgga gcagaaccat aatctgcttc cctgtaacgg aattcatagt aatttaaacc
     4441 tgctaacagg ctctttttgt caattccctg atcagccagt tttctcaaag tgccttttac
     4501 aacggataaa aattcaccct tctgctcttt atttgcattc ttagccacaa cagagaaata
     4561 aggctgtaaa ataccatttt catagccgcc cataatatcc tgaccaatat gtgcatcgat
     4621 cagagcctgc tttaatggtg ctcccggagc gctgatgagc gtgtagtcca ggatctggaa
     4681 tgcaacataa agaactggat ctatatctgt tcctaccact gtgttcagag ataagtaggt
     4741 gttgtcttct gtgccttcat tcgcagtaac agaataggtg gtctcttttt caactggttc
     4801 tgtaaatggc ttctgtaatg caatggaaga gtctaaggta aagtcctttt tgtcataggc
     4861 agataaatat tctttatcca gccataaaag cttttcttcc atatccatat cgccataaag
     4921 atagatatag ctgtttgccg gatgataata gtctttgtgg aattggatga actgctcata
     4981 gcttaagttg gggatcactg ccgggtcacc gccagactca ttgctgtacg gggtatctgg
     5041 aaaaaggact gatgaagtga aacgctccag cacgctttcc ggtgaggaaa aagcaccttt
     5101 catctcattg tatacaacac cattgacagt aagaggtgca tcctctgaag gcagttcata
     5161 atgccagcct tcctgtttga agatctttgg ctctctgtaa atggatggat ggaatacacc
     5221 gtctaaatat acatccatca ggttctggaa atctttatca ttgcaactgg ctaccggata
     5281 tacggtcttg tccggataag tcattgcatt taagaaggta tttaaggaac cttttactaa
     5341 ctctacaaag ggatctttta ccggaaactt ttcagaacct tccagtacgc tgtgctccag
     5401 gatatgaggc agtccggtac tgtccgcagg cggtgttctg aagccgattg aaaatacttt
     5461 gttgtcgtct tcgttgctca tgaggaacag tctggcaccg ctttttctat gttccagcac
     5521 cattccttcg gaattcatct cttctacaaa acgacggtct tttacctcgt aggcagaaag
     5581 gttttctact ttttcaagca tctgctgtct gttcattttg aattggtctc ctatcttgtt
     5641 tggttttcca tgaggctggg aaatcccttg cgtgagggat gtcgcgtccc ggtcactcac
     5701 aacctcgcac ttcgtgcttt actgcctgct atcgcaggct ggttgttcgt tcgttagtag
     5761 actcgcaaac acgcgcttcg cgctccactg tctgcttgcg caggctggtt gttcgttcgt
     5821 tagtagactc gcaaacacgc gcttcgcgct ccactgtctg cttacgcaga cggtttgctc
     5881 gttaagggcc caagaaaaac aaatgtctgc tttgcagacg cttgtttttc ttctggggcc
     5941 cttacatgtt ccccattatt ctgtccttta ctatggatgc acattcacgc acacacaaat
     6001 ggataaacag cacagggtct gactgggcgc tgatgccgta gacattttca aaaccccatt
     6061 tttttgcaat catgcaggct ctgtatacat ggaaattact ggtaaggaca cccacatcca
     6121 gaggtttgtc cgctgcaata agataaccgc cttttggttc ccgttgaaat gtcaggcggt
     6181 ttctttcttc tctctgatga cggatcactt ccctgtggct gtcaatgatg atcttgctgt
     6241 atgccagatt ttccactgtg cttacggaat gttcttccaa aagaagctga ctgggtctta
     6301 cgccgttgta taccagatac tggtacatag cctgggcttc tgatacaggt tcatccggtc
     6361 ctttgcctcc tgacagcacc agataggttt ccggattttt ctctgcatat tcaatggcct
     6421 tatccagcct cttttttaaa gaattgctta ctgtatgttc cttgactctg gctcccaaaa
     6481 cgatcacata gtcaagatcc ctttcttcac tggctgccgc tcccataaac accatgatac
     6541 aaagggctgc cataatgacc acaccggcaa tacaggtagt aacaatgctc accggcaccc
     6601 actgtgggat gcggtccata tttttcctgt aataccattt tccatataca agaaatacac
     6661 aaagagcagc aaagaaaagc cagataaagg caaaggaggt tcccatacct gcataaaaaa
     6721 caataaccat aaaatatgca gtacagatga tcgccagtcc caacaggatc atatctgcca
     6781 tagagcacct cctttttatc attcagctat attttaacaa ttattaatta tcctcttttt
     6841 tgcggagaat atcatattta tccacatctg ctcctaaatc tacccacagg atctgttttg
     6901 gatgtggagc actttcctgc tcatttccat cttcatcgca gatgctcttt acaactactt
     6961 ccagatttcg gccgtctggc ttcatgatct caatggtctc gcctacagag aacttatttt
     7021 tctgttcaat gcggcagcgg ccttttccgt ccgcctcttc cactgtgcca agataggtat
     7081 aattctttac ataagtattg ctgttgtaga tctgggcatc acttcccggc tttccaaagt
     7141 agaaacctgt agtaaattca cggtaggtac attttccgat ctcttccttg taccattcca
     7201 tatttgcctc ataaagcttc ggatctttca gataatcatc aatggctttg cggtaggttc
     7261 ttgctacagt cgctacatac aaagctgttt tcatacgtcc ttcgatctta aagctgtcaa
     7321 ttcccgcatc gatcagttcc ggaacatgct cgatcatgca aaggtccttg gagttaaaga
     7381 tataagtgcc tctttcattt tcatatacag gcatgtactc tcccggtctg gtctcttcta
     7441 caatggaata tttccagcgg catggatggg tacaggcacc ctggttggcg tcacgacctg
     7501 taaagaagtt actcagcaga catcttccgg aataggaaat gcacatagca ccatggatga
     7561 aactttcaat ctccatatca tccgggatat gagatcggat ctccttgatc tcagctaaag
     7621 aaagctctct tgcggaaacc acacgctttg cacccatacg gttccagaac agatatgtgc
     7681 cgtagttggt attatttgcc tgtgtgctta catgaagttc tgtatccgga agtactctct
     7741 ttgcgatctc aaatacacct ggatcagaaa tgatcagtgc atctggtccc acttccttta
     7801 attcttcaaa ataagcctcc acccctggca ggtcctgatt atgagccaga atattggctg
     7861 ttacatagac cttaacatca tgcgcatggg caaaggcaat tccctctttc atgtcctcta
     7921 aggagaagtt atgtgctttg gctctcannn nnnnnnnaag cctctcctcc tatatatacg
     7981 gcatccgcac cgtaaataac ggctgttttt aatacatcca gactgcctgc cggtattaat
     8041 aactctgtct ttctcattta tctatcttct cctatctgat cctggtactg atggtcactc
     8101 catctcctat agggatcacc gctgtttcaa attcttccat atgctttaat tcatacagat
     8161 actcacgcat cctgctgtgg atggtacggt tcctgcgctg cactgcaaaa cgggactgga
     8221 ctacatcccc atcctggagc acattatcgg aaataagcac ggctcctaca ggcatcaggt
     8281 ctaacagcag aggcagccag tttaaatact gtcctttcgc tgcatccata aataccagat
     8341 caaactgttt tccttttaat tctttcagcc acatatccgc atctccctct aaaaaggtaa
     8401 tgcggctttc ttccccggct tttctaaaat tttctttcgc ctcaggaata cgtttttcgt
     8461 atttttcaat ggtagtgata tggcagtttg ccggcatcac ctgacacatc tgtaacgcag
     8521 aataacctac agccgttccg atctccagaa tattttccgg tcttaatgct gctgtcaggc
     8581 ttctaagcag ggcggctgtc tcactgcgga taataggaac tccgtcttca atcgcctttt
     8641 tttcaattgt atccagaagt tctcccctgc ttgtttccag ggaatgaaga tattctgtaa
     8701 tcctgttatc tacgatcatt ttgtccgtcc tctgtcacat cttcgatcca gccgccttct
     8761 tcctcatctt caccagaata ggaatgcgca tctgcctggg cttctcccga tgaaagggtg
     8821 ctctttgcag gatctgctgc atttgcttct tctgtctgaa ggtctgactg acgtttttgc
     8881 tgatcacttt ctgatttttc agtactctga tctgcgctct tttgtgttgt ttttgtgctc
     8941 cctgatttat ccccggattt ttcgtcctct tcattttccg gcttttcgct cagtttttga
     9001 aggatctcct tggaagtcat ggaagtattg aacgtatagg ttcccgggta tatcttatca
     9061 taatcataaa aacgactctg gaataaaaag gcatagatat ttttgatcag accctttttc
     9121 tccagctcat cagctgcctg tgacacatct tccccgtctt ttaccactac agtaatatca
     9181 tgacccggag ctgcttccac tgcctctgca taaaacaccg catggccaaa agcatacccc
     9241 cggatcactg cttcatataa aagaaggatc aaaagggcaa gaagcatcag ctttaccgaa
     9301 atacggacga tcgccatggt tattttgttg atctctcttg tacggctgct catgcttctt
     9361 cgcctccttc ttacacttcc ataatgatcg gtaagatcat aggatttctc ttcatacgct
     9421 tccataagaa atcgcttaag ctgtctttaa taatgttctt gatcttgccc cagtcgctta
     9481 cgtggcggtc taagcagtct gctactgcat ctgcaaccac attgtgagct tcctctaata
     9541 aatcctcaga ctctcttaca tatacaaagc ctctggatac gatatccgga ccggaaagca
     9601 gctggttgga atgtttctcc agtgtcagta ctacaacgat gataccattc tgagccaggt
     9661 tctgtctgtc tcttaatacg atatttccta catcgccaac cccaagtccg tcaaccagga
     9721 tggcgccgca ggctacatga tcggttacct gacagctgtc ttcatccaaa gataccacat
     9781 caccggaatt taccagtaat gcattcccct tctgaacacc catgtactca gccagttcct
     9841 tctgcgccat tctgtggcgg aactcaccat gtaccgggat ggcaaatttc ggatgtacca
     9901 gggaatagat cagctttaag tcttcctgac atgcatgtcc ggaaacatgg gtatcctggc
     9961 agatcacctt tgcacccttt gcagaaagct cattgatcac atttgctact gccttctcat
    10021 ttccagggat cggagtagaa cttaatacta ccacatcacc aggtacgatg gataccttct
    10081 tgtggatgct tgctgccata cgggacagag cagccatgga ctcaccctgg cttcctgttg
    10141 tgatcagtac cgtagactca ggcggataat tctttaactg gtcaatatcg atcagagtgc
    10201 cttccgggat attgatataa cctaactcac tggcaatatc tatgatcgtc accatactgc
    10261 ggccttctac tactacctta cggccatatt tataagcagt atttactacc tgctgtacac
    10321 ggtctacgtt agacgcaaaa gtagctacga taatacggcg attggtatgt tccgcaaaaa
    10381 tagtatcaaa ggtctttcct acgctgcgct cagacatggt aaatccaggt ctggtagcat
    10441 tggtactgtc tgccagcata gcaagaacac cttttttacc cagctctgca aaacgctgca
    10501 gatcaatagg atcaccaaaa acaggtgtgt agtcgatctt aaagtcacct gtatgaagaa
    10561 caataccaac cggtgagaag attgcaaggg cggaagcgtc ctggatactg tgattggtct
    10621 taataaattc tacgcggaag catcccagat taacagactg tccatgcttc attaccttac
    10681 gcttggtagt ctttaacagg ttatgctctt ttaatttatg ttcaattaag gcaatggtca
    10741 gctttgtacc atagatcggc acattcacct gctgtaaaat atatggcagt gcgccaatat
    10801 ggtcctcatg tccatgagtg atcacaaaac cctttacctt atcaatattc tgctttaaat
    10861 aagatacatc cgggatcacc aggtcaatac ctaacatatc atctcccggg aatgcaagac
    10921 cgcagtctac aacaataatg gaatcttcat attcaaatgc ggtaatgttc ataccgatct
    10981 gctctaaacc gcctaaaggt atgatcttta atttactctt tgcttctttc tggttgtttc
    11041 tggaaggttt tcttacaggt gcttttctct ctcctgcttc ttttacagcc ggaagggacc
    11101 ttctgtttcc ctgctgtctt cctcttgcct gagcaggttt acgtctttct tctgttttcg
    11161 gctctgcagc tcctgcgttt tctgtttctt ctttttcttc cacttttaca gcttttggcg
    11221 ctctttgccc acgtctcggt cttctgtact gtggtcttct ggctggtttt gccccttctt
    11281 cacgattttc gccgttacca ttttcttctt ttttttctgc actgatctca ggttttacat
    11341 tttcattcac gttctcttct aaactcaagc tgattcctcc tacattattt ctctaattcc
    11401 acgtctgcat cctttaataa ctcttcaaaa atgcccataa ctccatccag ttccctgtca
    11461 tcttccacga actcaaataa agcttcactg tcattttcac cggatacatc ctttaaaata
    11521 taacattctc catcctcatc ctctggcgca tccgttacca gcagataatc ttttccatgg
    11581 atccttgttt cttccaatac gtaaaaatct acagattctc ctgaatctgt tatcatcgta
    11641 atgcagtctt ctctttcatt cattggctta tccattgttc tctccgtctt cttttttcgt
    11701 catggacaga tgatctaaat atccctgaag gatcattgct gctgcgatct ggtctaccga
    11761 ctcttttctg tgttcacggc ggacaccgct ttccataagg acacgctctg ctgctacagt
    11821 tgtaagacgt tcgtcccaca gctccacagg aagccctgtt ctacgctcta aagccgcttt
    11881 cagttcttct gacttctgtg cacggtctcc cactgtatta ttcatatttt taggataacc
    11941 aagaacgaac ttttctaccc catactcttt cgccagtgct tcgatcctgg caagggtctg
    12001 acgcagcttg ttttcatcct ttctggtaat ggtctctact gcctgtgcag taattcccag
    12061 gggatcgcac actgctaccc ctactgtttt tgagccataa tcaagcccca tgatcctcat
    12121 attcaattcc tccgcgcaca ctaaaactag tgtaccgcat tattgatatt tctccaaaca
    12181 gcttactcaa caatacagta caccaacaaa cacaaaaact gcgtttgtga tcatcattac
    12241 agaaaaaaat caacgcccat aaaaagtccc taaacagaga ctt
//
LOCUS       DBMDUA010000074        12139 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2682_length_12139_cov_3.400399, whole genome shotgun sequence.
ACCESSION   DBMDUA010000074 DBMDUA010000000
VERSION     DBMDUA010000074.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 12139)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12139)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12139
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2682_length_12139_cov_3.400399"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            12..395
                     /locus_tag="ACZPCL_07240"
     CDS             12..395
                     /locus_tag="ACZPCL_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009531526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR1162294.1"
                     /translation="MKIEHIALYANDLEGARKFFMKYLGAQSNDGYHNPRTNFRSYFL
                     SFDDGARLEIMNKPEMTDLPKDLVRTGYAHIAFSVGSKEKVDALTAQLKSDGYEIVSG
                     PRTTGDGYYESCIVAIEGNQIEITV"
     gene            480..950
                     /locus_tag="ACZPCL_07245"
     CDS             480..950
                     /locus_tag="ACZPCL_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024803.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1162295.1"
                     /translation="MKEEYYQIGDVSRITGISKDTLHFYTKIGLVTPDYINPKNHYHY
                     YSRWNMYQLDIIATCRNLKIPLEKVKEIISSRDNEKVVKLLMEYQSEALRLSQYYKQV
                     ADDISWYYEENERIKTQMSDCKVHIKHFGPETVIVGNQKRKESSYHANLQDVLI"
     gene            1379..1918
                     /locus_tag="ACZPCL_07250"
     CDS             1379..1918
                     /locus_tag="ACZPCL_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013703.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1162296.1"
                     /translation="MYQFFTEDAVTVLGIYYKIQSFFFILLLGFQQVLLPLFSYHYGA
                     GDFQRNREILKYSMMFSVGIMAVATGIFFLFPEKLVQIFSTESAIVAIGNYAFPVISV
                     SFVFAGLTIVITSYLQGIEHIKLSLFIIVLRQIILLVPLAWLFHFISLNAVWWTFPVT
                     EIIASIAGIFIIFISLKHQ"
     gene            2244..2720
                     /locus_tag="ACZPCL_07255"
     CDS             2244..2720
                     /locus_tag="ACZPCL_07255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008753574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR1162297.1"
                     /translation="MFRPIRKKKNEISTDEALALLQDSRRGVLAVNGDDGYPYAIPVN
                     YYFDKENRKIYFHGARVGHKVDALRACDKICFTVYGHETIKEEAWAPFMQSVVVFGRC
                     HLVEAGEKAMAMLKQFAMKYYPNEQMVDEEIAIAGKAVQIFEIEIEHLSGKEVQEK"
     gene            complement(2858..4360)
                     /locus_tag="ACZPCL_07260"
     CDS             complement(2858..4360)
                     /locus_tag="ACZPCL_07260"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR1162298.1"
                     /translation="MRKTFKHLAMAALASALIAGQATTAFAFKNDTNNGPGSGKSTEV
                     SLQAPGDNSDSSQSSGNNTSPTTIVEESSQSTDTSQSETSQTTETTTETTTDTTTAAE
                     TVTEDTSAQVPTNQAFLQVQLLRASDQVWSDPVRDDSVLSVGDAGFLSMCIYANNLPG
                     DVLYRTYSSSRGWTNWAMNGGHTDWSADCPVEAVQIRLNGIFGDRFDVYYRSNLSDGT
                     ECDWSKNGGTNGAMACGRIITGMRFSMWGKGVDGAAYKMDNPLVSAAPDGIQFVNGTP
                     VFSNGTGDNFTGWVWNDRDRYYVVDNAIVTGWQYIDGYKYYFEGDGKLVTDLEPYLNY
                     QGQFKIKINKQMNCLTIYIPDGDNGYIIPYKSFLCSTGDDTPLGEHKTPEKYRWRLMN
                     TDEYCQYLTRLDAGIPILLHSVIYERPDPYTLKAFTYNYLGATKSHGCIRLTTADSRW
                     IYEHCALGTSITVYESPIPGPFDRPVIKTMIPDTQTYDPTDANVPENGLQ"
     gene            4594..5640
                     /locus_tag="ACZPCL_07265"
     CDS             4594..5640
                     /locus_tag="ACZPCL_07265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HIR1162299.1"
                     /translation="MEKTMKEAVKTIKTKEQAVHIIYASNDGYAGHLAASLCSLLDNN
                     RHIKNLDIYVLSAQMCEAFQNRLKTLAAGFGRQLYAVELGDLKERFPYKIDTRGFDIS
                     AMGRLFAPEVLPETVTKALYLDCDTIVCSSIEKMYETPLGDCLAGMIMEPTVYKEMKE
                     SIDMDKDDAYFNSGVILMDVAGWRRENVLKKLLDFYGDHAGSLFACDQDTINGALNGR
                     ILPLSPRYNFFTNYRYFRYNTLVQLCDAYRAVGKKEFAAAKRRPTIIHFLGDERPWIG
                     GNHNYYGKWYWKYLAMTPWKDMPMQKGKFWYMQLWWCFNQMTRVCPALRLAISKRLGM
                     KVIDNRKKRQGKKK"
     gene            5661..6599
                     /locus_tag="ACZPCL_07270"
     CDS             5661..6599
                     /locus_tag="ACZPCL_07270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1162300.1"
                     /translation="MDADTNICAVILNYNDAQTTIKLTESWKNSKVIRNIIIVDNCST
                     DDSWEKLGEFKEEFASDHPDDPDKVQLHLFRTTENGGYGSGNQAGIDYAVQYLEPDYI
                     IIANPDVQVSDACILRVADALEKQEDGAVASAMVVDVKGQSSFSYWDLQSVWLDLLDT
                     GLVTRRLFRAFLKTPLEKLALAADGVSRMVGALPGSFFMVKTGCFTPGDLKELFDKHV
                     FLYYEEKILGQKLKQMGLKELLVTDAYYVHAHSVSIDKSYTKISDKQKLLHKSKLYYY
                     QHYLKLGEMELAVAKAFLAVVLTEVKFLTGVLKMRW"
     gene            6688..7374
                     /locus_tag="ACZPCL_07275"
     CDS             6688..7374
                     /locus_tag="ACZPCL_07275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1162301.1"
                     /translation="MDKVLLVIPAYNEGKNIERVVDHIKNDFPELDYVVVNDGSKDDT
                     ADICRRRGYNFIDLPVNLGLAGCFQTGMKYAWQKGYNYAVQFDGDGQHRPEYIQAMKE
                     KMDEGYDIVIGSRFVTEKKSWSMRMFGSRLIGTAIWLTTGAKIKDPTSGMRLFSRKMI
                     GEFAWNLNYGPEPDTVSFLIRQGAKIAEVQVTIDDRTAGESYLKPLTAASYMVRMLLS
                     ILVIQNFRKR"
     gene            7391..8707
                     /locus_tag="ACZPCL_07280"
     CDS             7391..8707
                     /locus_tag="ACZPCL_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR1162302.1"
                     /translation="MKNLTKQLLYKGDNVARANVVWNIAGSFVYAFASMVLSFLVIRM
                     AGEEKGGIFSFGYSTLGQQLFLVAYFGIRPFQITDGKGEYSFKEYREHRNITCMFALV
                     AGALFLTARTIAFRKGIGDYTAGKCMILLLLVIYKVIDGYADVYESEFQRQGCLYLTG
                     KSNFFRTVFSVAVFTVTLGAFKHLLFACLMAVAAQAMGVFLFNLDVIHALNGVDWTFT
                     KGKVTKLFKNTGFLFISVFLDFYIFSSAKYAIDARMNNAASGYFNLIFMPTSVIYMVA
                     NFVIRPYLTTLTNLWTEEKIAEFKKTLVRIAAVILGLTVLAVAGTLVLGKWALSIMEL
                     LMGGEKGTLTVYFGAFAGIVLGGGFYALANLMYYALVIMRKQRTVFFVYAAAAVAAFF
                     LSGGLVGAFGINGAALCYLLLMAGETAGFGFCTVRSCRSEEKETRQ"
     gene            8704..9696
                     /locus_tag="ACZPCL_07285"
     CDS             8704..9696
                     /locus_tag="ACZPCL_07285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1162303.1"
                     /translation="MTEERKSENALRKPRVDVVIPTYKPGKKFSRLLKMLEKQTYPIG
                     KIIVMNTEKAFWNEKGFEGISNLQVHHLTKAEFDHGATRNRGMRFSRADIVVFMTDDA
                     VPADEFLIEKLVGAFDQRGPEGEAVIMAYARQLPDKDCPLAERYTRSFNYPEESCLKT
                     KADLKRLGIKTFFASNVCCAYDREKFWFQGGFIKNAIFNEDMIFAGKAVMEDDYAIAY
                     VADAKVIHSHNYNCTQQFKRNFDLAVSQADHPEVFGGIRSESEGIRLVKQTAHYLSEQ
                     HKPWLIPGMFVKSGFKYMGYRMGKAYHMLPQWLVMKCTMNREYWLEKKEGGDRR"
     gene            9693..10091
                     /locus_tag="ACZPCL_07290"
     CDS             9693..10091
                     /locus_tag="ACZPCL_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HIR1162304.1"
                     /translation="MMTMMLRVVLIVVSVITMTFMMRKIRQAKVQIEAAMFWVIMALI
                     LVVFAVVPGAADLCARLLGIYSTPNFLFLFMIFLLIVKAFGMTIQLSQLESKQRELIQ
                     KMALEQKFLEEKEAELEEREAALETCGKGE"
     gene            10113..11894
                     /locus_tag="ACZPCL_07295"
     CDS             10113..11894
                     /locus_tag="ACZPCL_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HIR1162305.1"
                     /translation="MKKKLPVWLLWPFAVFLLGIWHLLDVRASVTETGHHWLYGWYGG
                     LFFVSMVILVGFGVLIFGKNWGFFDSVSSRCGNALVLKKELVSRKESGSKTECWKLPG
                     LYFGVVMCLGILYMLVLAPLSAPDEVSHYISAYELSNRFLGLPGTDEDGYIYIQAEDD
                     WIEDVNQEFRDWKEKSEQESLNGENGETEAGKPIILGQTLKEETYAAIHDGGAVSDQS
                     AEQSVAGESAGNSGKTAGLRVSRQPSVRTTPLAYVPQALGFTLARVLGLNSIALLYLG
                     RFLNLLLFAAVGVLTIKRIPFGKNVFFGVSILPMSLHLAASLSYDVVILAFTGYFTAV
                     CLDLAYKADTVKVKDVIALAVVMAVMGPCKMVYGAIAGFCLLIPVKKFGNWGKWTVSA
                     AAVLGSFLAAMAVVNLRTVAMYTQESDSYVAWAEEAGYSFAELLHQPVRVLQLFYNTV
                     AWQGESLYSGMLGESLGNRDAVLNTPYVVILLLTACLVILVLKKPGEEIFMKIKERLW
                     IWFICLVIFAALMFSMLLAWTPKSSNMVQGVQGRYMLPLLPVLLLTLKNQKVVRTGTD
                     DRLVLYTMMAADVYVILRIFAVVCLRL"
BASE COUNT         3529 a         2223 c         3053 g         3334 t
ORIGIN      
        1 tggaggcagt tatgaagatc gaacacattg cactttatgc aaatgacctg gaaggagcca
       61 ggaaattctt tatgaaatat ctgggtgcac agtcaaatga tgggtatcac aatccgagaa
      121 ccaattttcg ctcttatttt ctttcatttg atgatggcgc aagacttgag atcatgaata
      181 aaccggaaat gacggatctg ccgaaagatc tggtacgcac cggatatgcg catattgctt
      241 tcagtgtggg aagtaaggaa aaggtagatg ccttgactgc tcagttaaaa tctgatgggt
      301 atgagatagt cagtgggccg cggaccacgg gagatggtta ctatgaaagc tgcattgttg
      361 caatcgaagg caatcagatt gaaattacag tttaagtctg acaaatcaga tcacatttgt
      421 caggatggag cgtatatcgg caatagacgg gaaaaaatct taagggtaaa gacagaaaga
      481 tgaaagaaga atattatcag attggcgatg tcagcagaat tacaggaata tcaaaggata
      541 ccttacattt ttatacaaag atcgggctgg taacaccgga ttatataaat ccgaaaaatc
      601 attaccatta ttattccagg tggaatatgt accagttaga tattattgct acctgccgga
      661 atttaaagat accactggaa aaggtaaaag agatcatatc ctccagagat aatgaaaaag
      721 tagtgaagct gcttatggaa tatcagagtg aagccttaag gctcagtcag tattacaaac
      781 aggtagcaga tgatatcagc tggtattatg aggaaaatga acgcattaaa acacaaatgt
      841 ctgactgcaa agtacatata aagcattttg ggccggaaac agttattgta ggaaaccaga
      901 aacggaaaga gtcgtcttat catgcaaatc ttcaagatgt tttaatctag gaattccaga
      961 caacaaatct tgtccgcaga aaatatggat attttctgga tacggagaga tttttaggaa
     1021 atgaacttag aaaatatcga gaatatataa agctgccaga tggtgattac agcgctataa
     1081 agccagaaca tttatataca cttcctggcg gaaattatgc agtctgcaca atacctataa
     1141 aaaatgaaaa agccgatttt tcaccactga tccattggct gaaggaaaag cattacagaa
     1201 cagatgctgt ttttgcagag aaagtaggat ttcagctgtt caaatatatt tatcactact
     1261 actgtgagat aaaaatacac ctggtttccc aataaatcct gttgacagtg gagttactac
     1321 acggtttaaa ataccttctg aagaaaattc aggaggtttt ttgctatgga tgtagagaat
     1381 ataccaattt ttcacagagg atgctgtaac tgtattaggg atatattata aaatacagtc
     1441 attctttttt attttgctcc ttggcttcca acaggtatta ctgccattat tcagttatca
     1501 ttatggagca ggcgattttc agagaaacag ggaaattctg aagtactcca tgatgttttc
     1561 cgtgggtatt atggcagtgg caacagggat attttttctg tttcctgaga aacttgtaca
     1621 gatattttct acagagtctg cgatcgtggc aattggtaac tatgcatttc cagtcatttc
     1681 tgtcagtttt gtttttgcag gacttaccat cgttataaca tcctatctac aggggatcga
     1741 acatataaag ttaagtcttt ttatcattgt gttacgccag atcattcttc ttgttccgct
     1801 ggcgtggtta tttcatttta taagtttaaa tgcagtgtgg tggacatttc cggttacaga
     1861 gatcattgcc agtatagcag gtatatttat aatatttatt tcattaaaac atcagtaaaa
     1921 taatgtagag taaaaattct ggacaatttt cttgttttat gttatataat aaatcatgac
     1981 ttttttctgc caccgggtag aaaaaaataa gcgtcagagt ttctatcgtt aggtcttaga
     2041 agatagaaac catggcgctt tattttttat gtactaggaa attccgttgg aactccctag
     2101 tacacaaaaa ggcacgacct gccaacgatg cgcactcgcg cgatgaggaa gtttgtcgca
     2161 aacgaccgcg ctcggcgcga gaatgtgcca aggcacattc ttatttataa acctataaaa
     2221 atcaagaaag ggagagaaaa ataatgttca gacctatcag gaaaaagaaa aatgagatca
     2281 gcacagatga agcactggct ttgctgcagg acagccgcag aggcgttctt gccgtaaatg
     2341 gtgatgatgg ttatccttat gcaataccgg tcaactatta ttttgataag gaaaacagaa
     2401 aaatttattt tcatggagca cgtgtgggac ataaggttga tgcgctgcgt gcctgtgata
     2461 agatatgttt cactgtttat ggacatgaaa cgatcaaaga agaagcctgg gcaccattta
     2521 tgcagagtgt agtggtattt ggcagatgcc atctggtaga agccggagag aaggctatgg
     2581 caatgttgaa gcaatttgcc atgaaatatt atccaaatga acagatggta gatgaggaga
     2641 tcgcaatcgc aggtaaagca gtacagatct ttgagataga aatcgagcat ttaagtggta
     2701 aagaagtgca ggagaaataa aactgcggcg gtgtgatctg atatagaggt ttacggtata
     2761 aaaaatacct cccggttcag atgatataaa attcatctgt ttcaggaggt atttttatat
     2821 acatacaaga tctgttatta tcaactgtta acagatatta ctgtaatcca ttctccggta
     2881 cgtttgcatc agtaggatca taggtctgtg tatccgggat catggtcttg atgaccggac
     2941 ggtcaaatgg acctgggatc ggggattcgt atacagtgat ggaagttcca agtgcgcagt
     3001 gttcgtagat ccagcgggaa tctgcggtag tcagacggat acatccgtga gacttggtag
     3061 cgcctaaata gttgtaagta aatgctttta aggtatacgg atctggtctc tcataaataa
     3121 cagagtgaag aaggatcggg atacctgcat ccagacgggt caggtactgg cagtactcat
     3181 cggtgttcat cagtctccag cgatatttct caggagtctt gtgctcacct aaaggagtat
     3241 catcaccggt ggagcacagg aaggacttgt atgggatgat atagccatta tcaccatctg
     3301 gaatataaat ggtcaggcag ttcatctgct tgttgatctt gatcttaaac tgaccctgat
     3361 aatttaagta aggctccagg tcagttacca gcttgccgtc accttcaaaa tagtacttgt
     3421 agccatctat gtactgccat ccggttacaa tggcattgtc aactacataa taacggtctc
     3481 tgtcattcca tacccatccg gtaaagttgt cgccggtacc attgctgaat acaggagtgc
     3541 cgtttacaaa ctggatgccg tcaggagcag cggatactaa tggattgtcc atcttgtaag
     3601 cagcgccgtc aactcctttg ccccacatgg agaagcgcat gccggtgata atgcggccgc
     3661 aggccatagc gccgttggtg ccgccgtttt tagaccagtc acattctgtt ccgtcagaaa
     3721 gattggagcg gtaataaaca tcaaaacggt cgccgaaaat accatttaaa cggatctgta
     3781 cggcttctac agggcagtca gctgaccagt ctgtatgtcc gccgttcatg gcccagttgg
     3841 tccagccacg ggaactgctg taggtacggt aaaggacatc acctggaaga ttgtttgcat
     3901 agatgcacat acttaaaaat cctgcatcac ccacagaaag aacagagtca tcacggaccg
     3961 gatcactcca tacctggtca gaggcacgaa gaagctgtac ctgtaagaaa gcttggttgg
     4021 tcggaacctg ggcagaagtg tcttctgtaa cggtttctgc tgcagtagta gtatctgtgg
     4081 ttgtttctgt agtagtttcc gtggtctgag aggtctcaga ctgggaagtg tctgttgact
     4141 gggaagactc ttctacaatg gtagttgggg aagtattgtt tccagatgac tgggaactgt
     4201 cactgttatc cccaggtgcc tgtaaagata cttcagtgga ttttcctgaa ccaggaccat
     4261 tgttggtgtc atttttgaat gcaaaagcag tggtagcctg tcctgcgatc agtgcagagg
     4321 caagagcagc cattgccaga tgtttaaaag ttttacgcat aatagaacct cctacggtta
     4381 gttacatgtt attgtctgca taaaatgaga acaataacca tactgccttt aatttatcat
     4441 aattggggga aaaatgccat ataggattta cattttagga cccgtattgt aaaatcgtaa
     4501 ataaaatgac catggaatgt aagaaattaa atgtattatg gaacagaagg atctgtcgtc
     4561 ggcaggcgac atcatcatga ataaaacatg agaatggaaa agacaatgaa agaggctgta
     4621 aagactataa aaacgaaaga acaggcggtc cacattatct atgcttccaa tgacggctat
     4681 gccggacatc tggcggcatc tttgtgctct ttgctggaca ataaccgcca tataaaaaat
     4741 ctggatatat atgtgctttc tgcccagatg tgtgaagcgt ttcaaaaccg gcttaaaacg
     4801 ctggcagccg gttttggacg tcagctttat gcggtggaac tgggagatct gaaagaaaga
     4861 tttccttata aaatagatac aagaggcttt gatatcagtg ccatggggcg gctttttgcc
     4921 ccggaggtac tgccggagac tgtgactaag gcattgtacc tggactgtga taccattgtc
     4981 tgcagttcca ttgaaaaaat gtatgaaaca ccgctgggag actgtctggc aggcatgatc
     5041 atggagccca cggtctataa agaaatgaaa gagtccattg atatggataa agatgatgcc
     5101 tattttaact ccggtgtgat cttaatggac gtggctggct ggcgcaggga aaatgtgctg
     5161 aagaaactgc tggattttta cggggatcat gcaggaagcc tttttgcctg tgatcaggat
     5221 accattaacg gggcattaaa tgggcgcatt ttacctcttt ccccaaggta taatttcttt
     5281 accaattacc ggtatttccg ttataataca ctggtgcagc tttgtgatgc ttaccgggcg
     5341 gtaggtaaaa aagagtttgc tgcagctaag agaagaccaa cgatcattca ttttctggga
     5401 gatgagcggc cctggatcgg tggcaaccac aattattatg gaaaatggta ttggaaatat
     5461 ctggctatga ccccatggaa ggacatgcct atgcaaaagg gtaagttctg gtatatgcag
     5521 ctttggtggt gctttaacca gatgacaagg gtatgtccgg ctctgcggct ggccatcagc
     5581 aaacggctgg ggatgaaggt tattgataac cgtaaaaaac gtcaggggaa gaaaaaataa
     5641 aacagtgaga gaaaggaact atggacgcag acacgaatat atgtgccgta atattaaatt
     5701 ataatgatgc acagacaacc ataaaactta cagaaagctg gaaaaacagc aaagtgatcc
     5761 ggaatatcat tattgtagat aactgttcta ccgatgactc ctgggaaaaa ctgggagaat
     5821 ttaaagaaga atttgcttca gatcatccgg atgatccaga taaagtgcag cttcaccttt
     5881 tcaggacaac agaaaacggt ggctacggaa gcggaaacca ggcggggatc gattatgcag
     5941 tccagtatct ggaaccggat tatattatca tcgcaaaccc ggatgtgcag gtcagtgacg
     6001 cctgtatcct gcgtgtggca gatgctttag agaagcagga ggacggagct gtagcttctg
     6061 ccatggtagt ggatgtaaag ggacagtcct ctttcagcta ctgggatctc cagtctgtgt
     6121 ggctggatct tttagatacc ggtcttgtga ccaggaggct gttccgtgcc tttttgaaaa
     6181 cgccgttaga aaagctggct ctggcagcag atggtgtcag tcgtatggtg ggggctcttc
     6241 caggctcctt tttcatggtg aaaacaggct gttttacgcc aggagattta aaagaactgt
     6301 ttgataaaca tgtatttctc tattatgaag agaaaatatt aggacagaaa ttaaaacaaa
     6361 tgggattaaa agagctttta gtgacagatg cttattatgt acatgcacat tctgtaagca
     6421 ttgataaaag ctatacaaag ataagtgata aacagaagct ccttcataag agcaagttat
     6481 attactacca acattacctg aaactgggag aaatggaact ggctgtggca aaagcatttc
     6541 tggcggtggt ccttacagaa gtaaaatttc tcacaggtgt cttgaagatg cgctggtaat
     6601 gatggataat ggatatcagg ataaaaggga aagtgttaca aatcagcgca tggctgaact
     6661 gtaacagagt attgaggaac agagacaatg gataaggtac ttttggtcat accggcctac
     6721 aatgaaggaa aaaacattga gagggtggta gaccacatta aaaacgattt tccggaactg
     6781 gattatgtgg tggtcaatga cggctccaaa gatgatacag ccgatatctg tcgcaggcgt
     6841 ggctataatt ttattgacct gccggtaaat ttaggattgg ctggctgctt tcagaccggt
     6901 atgaaatatg cctggcagaa aggctataat tatgcagtac agtttgacgg cgacggacag
     6961 caccgcccgg aatacattca ggcaatgaaa gaaaaaatgg atgaaggata tgacattgtc
     7021 attggttccc gttttgtaac agagaagaaa agctggtcca tgcgtatgtt tggaagccgc
     7081 ctgatcggaa cggcaatctg gctgactaca ggggcaaaga tcaaagatcc tacttccggg
     7141 atgcgtcttt tcagccggaa gatgatcggt gaatttgcct ggaacttaaa ttatgggccg
     7201 gaaccggata ctgtatcttt cctgatccgc cagggagcga agatcgcaga ggtgcaggtc
     7261 accattgatg accgtacagc aggagaaagc tatttgaaac cattgacagc tgcaagttat
     7321 atggtgcgca tgctgttatc catactggtc atccagaatt tcagaaagcg ttaaatataa
     7381 gggattagat atgaaaaatc ttacgaagca gttattatat aagggagata atgttgcaag
     7441 agccaatgtc gtgtggaata ttgcaggcag ctttgtctat gcttttgcaa gtatggtcct
     7501 ttcttttctg gtcatccgca tggcaggaga ggaaaaaggc ggtattttct cctttggtta
     7561 ttctacgctg ggccagcagc tgtttttagt ggcctatttt ggcatccggc ctttccagat
     7621 cacagacgga aagggagagt attcctttaa ggaatacaga gaacatagaa atataacctg
     7681 tatgtttgca ctggttgcag gtgcgctgtt tctcactgca aggaccattg ctttccgtaa
     7741 agggatcggg gattatacag cgggaaaatg tatgatcctg cttcttcttg tgatctataa
     7801 ggtcattgac ggatatgcag atgtgtatga gtcggaattt cagcgtcagg gatgtttata
     7861 tcttacagga aagtctaact ttttcaggac tgttttttct gtagcagtgt ttacagtgac
     7921 gctgggggca tttaaacatc tgctttttgc ctgtcttatg gcagtggcag cccaggctat
     7981 gggtgtgttc ttatttaacc tggatgtaat ccatgcatta aatggcgtgg actggacctt
     8041 tacaaagggc aaagtgacga agctgtttaa aaatacggga tttttattca tctctgtatt
     8101 cctggatttt tacattttct cttctgcaaa atatgccatt gatgcccgga tgaacaatgc
     8161 ggcatcggga tattttaact tgatttttat gcctacctct gttatttata tggtagccaa
     8221 tttcgtgata cgtccgtacc tgaccacact tacaaatctc tggacagaag aaaagattgc
     8281 tgagtttaaa aagacactgg tgcgtattgc ggctgttatt ttagggctta ctgtacttgc
     8341 agtggcaggt acacttgttt taggaaaatg ggcactttcc attatggagc ttcttatggg
     8401 aggcgaaaag ggaacgttga ccgtttattt tggggcattt gcagggatcg tccttggagg
     8461 cggtttttat gcactggcaa atctgatgta ttacgcactg gtgatcatgc gaaagcagag
     8521 aaccgtgttt tttgtatacg cagcggcagc tgtagcagcg tttttcctgt caggaggtct
     8581 tgttggggct tttggtatta acggggcggc gctgtgttat ctgctgctga tggcaggaga
     8641 aacggcaggt tttggatttt gcactgtaag atcctgcagg agtgaggaaa aggagacaag
     8701 acaatgacag aggaaagaaa gagtgaaaat gctcttcgca agccacgggt cgatgtggtg
     8761 attcctacct ataaaccagg taagaaattt tccagacttt taaaaatgct ggagaaacag
     8821 acctatccca tagggaagat cattgtaatg aatacagaaa aggcattctg gaatgaaaag
     8881 ggctttgaag ggatcagcaa tcttcaggtc caccacctga caaaggcgga atttgaccat
     8941 ggtgccacaa gaaatagagg catgcggttt tcacgggccg atattgtggt gtttatgact
     9001 gatgacgcag ttccggcaga tgaattttta attgaaaagc tggtgggagc atttgaccag
     9061 agaggtccgg aaggagaagc ggtgatcatg gcttatgcca gacagcttcc agataaggac
     9121 tgccctctgg cagagcggta tacacgttcc tttaactatc cggaggaaag ctgcttaaag
     9181 acaaaggcag atttaaagcg tctgggcatt aaaaccttct ttgcttccaa tgtatgctgt
     9241 gcctatgacc gggagaaatt ctggttccag ggcggattta ttaaaaatgc cattttcaat
     9301 gaagatatga tatttgcagg aaaggcagtc atggaagacg attatgccat tgcctatgtg
     9361 gctgacgcaa aggtgatcca ttcccacaat tataactgca cccagcagtt taagagaaac
     9421 tttgacctgg ctgtgtccca ggcagaccac ccggaggtat ttggcggtat tcgctcagaa
     9481 agtgaaggca tccgtctggt gaagcagaca gcccattatc tgtcagaaca gcataaacca
     9541 tggctgatcc cgggaatgtt tgtaaaaagc ggttttaagt acatgggata ccgtatggga
     9601 aaggcatatc acatgcttcc ccagtggctg gtcatgaaat gtaccatgaa ccgggaatac
     9661 tggctggaga aaaaagaagg cggtgacaga cgatgatgac aatgatgctt cgcgtggtgc
     9721 tgatcgtggt ttctgttatc accatgacat ttatgatgcg caagatacgc caggcaaagg
     9781 tgcagatcga agctgccatg ttctgggtga tcatggcgct gatcttagta gtatttgcag
     9841 tggttccagg agctgcggat ctttgcgcaa ggttgttagg catttattcc acaccaaact
     9901 ttctgttcct gtttatgatc tttttgctga tcgtaaaggc atttggtatg accatccagt
     9961 tgtcccagct tgagagcaag cagagggagc tgatccagaa aatggctctg gagcagaaat
    10021 tcctggaaga aaaagaggca gagctggaag aacgggaagc tgcgttagaa acctgcggga
    10081 aaggtgagta aaagataaaa gggaaagaaa tgatgaaaaa gaaattacct gtctggcttt
    10141 tatggccttt cgccgtgttt cttctgggaa tatggcattt gctggatgtg cgggcatctg
    10201 taacagagac aggccaccac tggctttatg gctggtatgg gggcctgttt ttcgtgagta
    10261 tggtgatatt ggtcggcttt ggggtgttga tatttggaaa aaactgggga ttttttgaca
    10321 gcgtttccag tagatgcggg aatgcactgg ttttaaagaa agaacttgtc tcacggaaag
    10381 aatctggttc aaagacagaa tgctggaaac ttccaggtct gtactttgga gtggtcatgt
    10441 gccttggtat tttgtatatg ctggtcctgg cgcctctttc agcaccggat gaagtcagcc
    10501 actatatcag cgcctatgag ctttccaacc gcttcttagg actgccggga acggatgaag
    10561 acggttatat ttacatccag gcagaagatg actggatcga ggatgtgaac caggaatttc
    10621 gggactggaa ggaaaaaagt gagcaagagt ctttgaacgg ggaaaatggg gagactgagg
    10681 caggaaaacc tattattctt ggacagacct taaaagaaga aacttatgcg gcaatccatg
    10741 acgggggcgc ggtcagtgat cagagcgcag aacagtctgt ggctggtgaa agcgcaggca
    10801 acagtggcaa aacagccggt ctgagggttt cccgccagcc gtccgttcgg accaccccac
    10861 tggcctatgt acctcaggct ctgggtttta ctctggcaag agtacttgga cttaacagca
    10921 tcgccctttt atatctgggc cggtttttga atctcctgct ttttgcggca gttggtgtgt
    10981 tgaccataaa aaggattccc tttggaaaaa atgtattttt cggcgtgtcc attttaccaa
    11041 tgagccttca tctggcggct tctttatctt atgatgttgt gatcctggca tttacaggat
    11101 attttacagc agtgtgcctg gatctggcct ataaagcgga cacagtgaaa gtgaaagatg
    11161 tgatcgctct ggctgtggtc atggcagtta tgggtccctg taaaatggta tatggtgcca
    11221 ttgcaggctt ctgtctgctg atcccggtaa agaagtttgg caactgggga aaatggacgg
    11281 tctcagcagc agctgttctt ggaagctttt tagcagcaat ggcagtggta aatttaagga
    11341 cggttgccat gtatacacag gaaagtgaca gctatgttgc ctgggccgaa gaagcgggat
    11401 attcctttgc agaattgctc caccagccgg tccgtgtact gcagctgttt tataatacag
    11461 ttgcatggca gggagaaagc ctgtattcgg gaatgttagg agagtcctta ggcaaccggg
    11521 atgcagtgtt aaatacccct tatgtggtca ttttattact tacagcatgc ctggtgatcc
    11581 tggtccttaa aaagccggga gaagagatct ttatgaaaat aaaagaacgg ctctggatct
    11641 ggtttatctg tctggtgatc ttcgcagcac tgatgttttc catgcttctt gcatggacgc
    11701 ccaaaagctc caacatggtc cagggagtcc agggaagata catgcttcca ctgcttcctg
    11761 ttttgctgct tacccttaaa aatcaaaagg tagtgcggac aggaacagat gaccggctgg
    11821 tcctgtatac tatgatggct gcagatgtgt atgtgatcct gcgtattttt gcagtggtgt
    11881 gcctgagatt gtaaggattt taaccctgta cagcagataa catgcggtga acatgttgcc
    11941 ggcaagggga taaacagttg cagacttgtt gataacgtgg taaaatttcc agatgaatgt
    12001 ggaaaattta taatcgataa atagaaacac aaaattagaa aaataaagaa gataaaggag
    12061 aaacattatg ggaaagatta aagtaacaac ctgtgatatt gaaggacttt atgtgatcga
    12121 gcctactgta ttcaaagac
//
LOCUS       DBMDUA010000075        11867 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2744_length_11867_cov_3.336138, whole genome shotgun sequence.
ACCESSION   DBMDUA010000075 DBMDUA010000000
VERSION     DBMDUA010000075.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11867)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11867)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11867
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2744_length_11867_cov_3.336138"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /gene="cobI"
                     /locus_tag="ACZPCL_07300"
     CDS             <1..619
                     /gene="cobI"
                     /locus_tag="ACZPCL_07300"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HIR1162306.1"
                     /translation="IFCPEKKKGAGSFAFDIIKEHIKDSKAQIVNLEYPMHYKGDELK
                     KMWEENGRLISEYLKGDKTGAFITLGDPSVYSTFMYTLPYIEAFGTETEVVPGIPSFC
                     AAAAISKIPLTAWDEDLVIAPVRKNNAADLEKLLTEHENVVLMKPSTDSEALVSAIQA
                     SGRENSFVLVEKAGTKEQRLIREYQTLKEEPVPYLSLMILKSQKQ"
     gene            complement(600..1439)
                     /locus_tag="ACZPCL_07305"
     CDS             complement(600..1439)
                     /locus_tag="ACZPCL_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1162307.1"
                     /translation="MDFKYEMVVPNEDLPCKFFIFEGKNGNYRRANHWHQSVEIFLVL
                     EGELKFYINNQSQPLSAGELIIVNSNEIHSIEAPKPNLTLVLQIPVSYFTPYMGKEDY
                     AIFVSQSQEKNRALAVLIEKMYHVYTKKEKAYLLKMQSLFYELLYQMATEFMKTEADE
                     GNLRQKRHLDRLSQITSYMKKHYDQPITLESVAETFGFSPNYLSRMFRLYAGISYKTY
                     LLNLRTEYAFREMLHTDRSLNDIAINNGFPNSRAFAKSFLKRYDCLPGEYRKKIQLTA
                     SET"
     gene            1672..3198
                     /gene="xylB"
                     /locus_tag="ACZPCL_07310"
     CDS             1672..3198
                     /gene="xylB"
                     /locus_tag="ACZPCL_07310"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR1162308.1"
                     /translation="MNYLLGIDVGTSATKTVLFDEKGTVIASASQEYPLYQPENGWAE
                     QKPEDWRDAVLKTIKEVVSQSGVDKDAVKGIGISGQMHGLVMLDEAGEVIRPSIIWCD
                     QRTGAEVEDMLKIMPREEWIRITANPPLTGWTAAKILWVRKNEPENYNRCRHILLPKD
                     YIRYILTGVFATEVSDASGMQLLDVPGRCWSDEVLKALNIDKNLLGKVHESCEATGTL
                     LPDVAKELGLSEKCIVAGGAGDNAAAAVGTGVVKDGTAFTTIGTSGVVFAHTSKPAID
                     PKGRIHTCCCAVPGAWHMMGVTQAAGLSLKWFKDQFCQDYVKEAEAKGVDVYDQINQD
                     VASVAPGSDRLLYLPYLMGERTPHLDPDCRGVFFGLSAIHTRKHLLRAVMEGVAYSLC
                     DCNEILQEMGVKVDCMKACGGGGKSPVWRQMLADLYNCSVERVVQDEGPALGVAILAG
                     VACGLYESVEKACDQLITRRDSTEPKAEDVAVYEKYHRLYQKLYTSLKNDYKELAELS
                     "
     gene            3195..3818
                     /locus_tag="ACZPCL_07315"
     CDS             3195..3818
                     /locus_tag="ACZPCL_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4867 family protein"
                     /protein_id="HIR1162309.1"
                     /translation="MIKQISVMEPEFKKYGKVIGGYDLKEILQKMEETPCPDDVVYVA
                     SVKELEDLKIAEELADGVYGQMPIQIGYCNGHNRKLNALEYHRDSEINIAVSDMVLLL
                     AKEEEVEDDYTLDTSKVKAFFVPAGTMIEVYATTLHYAPCQTSDKGFRCVVVLPEGTN
                     GELSHKPENKSGEEKLLAAVNKWLIGHAEGGLPEGSFIGLKGENTEV"
     gene            3881..5368
                     /locus_tag="ACZPCL_07320"
     CDS             3881..5368
                     /locus_tag="ACZPCL_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HIR1162310.1"
                     /translation="MFENIPKVKVGIVAVSRDCFPESLSVNRRKALVDAYKAKYDPED
                     IYECPVCIVESEIHMVQALEDIKKEGCNALVVYLGNFGPEISETLLAKHFDGPKMFVA
                     AAEETGNNLVSGRGDAYCGMLNASYNLQLRNIKAYIPEYPVGTAAECADMIHDFLPIA
                     RTVIGLSDLKIISFGPRPLNFLACNAPIKQLYNLGVEIEENSELDLFEAFNKHAGDPR
                     IAEVAADMEAELGAGNKKPEILAKLAQYEITLLDWIRDHRGYRKYVAIAGKCWPAFQT
                     QFGFVPCYVNSRLTGRGIPVSCEVDIYGALSEFIGTCISQDAVTLLDINNSVPEDLYN
                     EDIKGKFNYDLKETFMGFHCGNTNSKKLAFCEMKYQFIMARSLPEEVTQGTLEGDIMP
                     GDITFYRLQSTADNILRAYVAQGEVLPVATRSFGSIGIFAIPQMGRFYRHVLIEKGFP
                     HHGAVAFGHFGKSLYEVFKYIGVDVEEIGFNQPAGMLYKTENPFA"
     gene            5758..7785
                     /locus_tag="ACZPCL_07325"
     CDS             5758..7785
                     /locus_tag="ACZPCL_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1162311.1"
                     /translation="MKYQIKDGTVTLGGETILSHIDFEIQGNQKIAVVGRNGAGKTTL
                     LRLIAGELSLDRDDRRQGPGITASRQLTVEMLGQQALAGEERTVEELVMLNCPAKGPF
                     DRECFEYEREYDTLFTGLGFQKEDKKRSVAAFSGGQKTKIALIQLLLKKPDLLLLDEP
                     TNHLDMETASWLEGYLRQYPGAVVMVSHDRFFMDRTADIVYELEQGKLTRYPGNYTNY
                     REQKRKNYEIQMKSYLRQQEEIERQEELIKRFKNKPSKAAFARSRKKILERMLPVEKP
                     REDMAHIFTGTITPLIPGSKWVFEAEHLKIGYDKHSLLELSLRIRKGQKIGILGVNGS
                     GKTTFLKTVAGFLPAVAGECALGNNITIGYFDQYSAAIQSEKTVVEHFSDLFPSLTDK
                     EVRTILGAYLFKGKDGAKRVDDLSGGEKARLVLAELLQSRPNFLILDEPTNHMDIQAK
                     ETLESAFQAYEGTILFVSHDRYFIRQVAKSVLIFENNAAFYYPFGYEHYLERKEKEAS
                     GEPLAARIKAEEQALIEGLKAVPRAERHRLREIPEEEAYDQWRLRLAAEAIENAAEQT
                     EEIWQQILEKNGKREEWELEHWDQWLNEIADKNEDAACGEKQEKQAEEELSAFTEQYE
                     NAQSAWTDACLFWYDIWCEAHPEPVMDPGTSDTDGSVPQDTESSKTDGSEI"
     gene            complement(7793..9766)
                     /locus_tag="ACZPCL_07330"
     CDS             complement(7793..9766)
                     /locus_tag="ACZPCL_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR1162312.1"
                     /translation="MKHKLLILTRNPVAKVGIASYLSSLFGQYLTIVSTRIADLKPED
                     MEDAGCILYTAGELKEQMPFSIPDSVKQLVCMRTFNHTYLQRILQIPPGSFVYLVNDT
                     VDTTYEIMEQLKEYGFSQYHFLPYLPGTGEVRKDIQYCVTPGEVHLVPSFIRQVVDIG
                     NRIVDISTINELIAVFKLPASLADEVTKNYLNHIIQIQKLSNQQLSQALDMKEITRGI
                     LENASEGLCLLNQSGKIELCNSLFLKMTGIKERDIQEKDFSRLLKEAGIIYDYPSQND
                     GIAVHNGEEAFRMWFQDFSIADSRQVRLIHTNPLQDAQFPTRITKLCDFSYFSTVNPL
                     QLHMLDTARRVSVNDFPIIIEGESGTEKELLAQAIHQNSRRHNGPFISLNISSLKSES
                     AEAALIGSYEKNENGVHKKIGVLEAARGGTLLINNLQYADPGLQRLLLSILKNGFFIP
                     LGNGSSPQPLELRLIVTSVSDLYSRVLEGKFLDELFFLLSTVSLYTIPLRQRRNDIPL
                     LLETFLKRSFHEPGLSSRELLTEPVLTFLNQYDWPGNVLELKNVCTYFSCIYHREPLS
                     LSDLPGYILNQIRKHEQQLDITEHRILSLIAANPRSGRSFLCSRLKEEGIQLSEANIR
                     TSLQRLASAGYIKVHRTRGGCEITELGGLVNTQ"
     gene            10042..11673
                     /locus_tag="ACZPCL_07335"
     CDS             10042..11673
                     /locus_tag="ACZPCL_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012706.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1162313.1"
                     /translation="MKKRALLVALAAAMAASLAGCGGSSASSTTAAGSAAESSEAAGN
                     TAADASGKDSLVIVTSSDVLSMDPYRYDEGPTNQIMLHIYEAMVAQNADMSFGPCLAE
                     SWETSEDGLTWTFHLREGVKFHDGDVMDSGDVVASINLASNPDSPSAYSSYTSSFESV
                     EAPDANTVVIKTEKPNPLMLFDVAQLYVLKQEIAEVGTEEQVADNVIGTGRYKFVEQV
                     KEDHIDLAANEDYWGEVPAIKNVRFRPITNEATRTATMLTGDVDFTIDVSARDIDRLD
                     ATEGISVIKQKGLREIYLNLDSREDSPLFPGQKNPMSDVKVRQAMYLAIDEGTIIKNI
                     MNGCAYEMNSVVPEDYVGYTEVTREAYDPEKAKQLLAEAGYPDGFEVTLDAPNDRYMN
                     DEQIAQAVAGYLEKVGIKVNLNLMPKANFFSYIKPLENKSMFLMTGWSDASGDGLSLM
                     HDMLYTYNREAGDGGVNRGHYSNAQVDALIDQAFTEMDDTKRGELVAEADKIAREDYA
                     YIPLHFEQDTYAIKDTLNYTPRMNKYVYAWEFSYK"
BASE COUNT         3449 a         2403 c         2978 g         3037 t
ORIGIN      
        1 gattttctgt ccggaaaaga aaaaaggagc aggtagtttt gcctttgata ttataaagga
       61 acatataaaa gacagtaaag cccagatcgt aaatctggag tatcctatgc attataaagg
      121 ggacgagctg aaaaaaatgt gggaagaaaa cggacgcctg atctctgaat atttaaaagg
      181 agataaaaca ggagctttta ttactttagg cgatccctct gtatacagta cctttatgta
      241 tacccttcct tatatagaag cttttggcac agagacagag gtagttccgg gaattccatc
      301 cttctgtgca gcagcggcta tttcaaaaat ccctcttact gcctgggatg aagatctggt
      361 gatcgcaccg gtccggaaaa ataatgcagc ggatctggaa aaacttctta cagaacatga
      421 gaatgtagtg cttatgaagc cttccacaga cagtgaagcc ctggtttcag ctattcaggc
      481 aagtggaagg gaaaattcct ttgtactggt agaaaaggcg ggaaccaaag agcagcgtct
      541 gatccgggag tatcagacct taaaagagga gccggttccc tatctgtctc tgatgatcct
      601 taagtctcag aagcagtaag ctgtattttt ttcctgtatt ccccgggaag acagtcgtag
      661 cgctttaaaa aggattttgc aaaggcacgg ctgttgggaa aaccattatt aatggcaatg
      721 tcatttaaac tgcggtctgt gtgtagcatt tcacggaatg catattcggt gcgcaggttt
      781 aaaagatagg ttttgtaact gatccctgca tagaggcgga acatgcggga taaatagttg
      841 ggtgagaagc cgaatgtttc agctacactt tccaaagtga tcggctgatc atagtgtttc
      901 ttcatataag aagtaatctg ggagagacgg tctaagtgcc gtttctgccg cagatttccc
      961 tcatctgcct ctgttttcat aaattccgtt gccatctggt ataaaagttc atagaagagg
     1021 ctttgcattt ttaaaagata ggccttttct ttttttgtat atacatgata cattttttca
     1081 ataagaacag ccagcgcgcg gtttttctcc tgggactggc ttacaaatat ggcatagtcc
     1141 tcttttccca tataaggggt aaaataagaa accgggatct ggagcaccag ggtcagattg
     1201 ggttttggcg cttcaatgga gtggatctca ttggaattga caatgatcag ttccccggca
     1261 cttaagggct gggactggtt gttaatgtaa aatttcagtt ccccttccag caccaggaag
     1321 atctccacag actggtgcca gtggttggcg cggcggtaat tgccgttttt tccttcaaaa
     1381 atgaaaaatt tacagggcag gtcttcgttt ggaacgacca tttcatattt aaaatccatc
     1441 ctgtagcttc ctttccgtaa aaattccagg tgtacataag gcttaaaaag ccggtcagaa
     1501 cacgcagatc ctgcaataaa gtataccata tgataagaat gatgtaaaag gataagaaag
     1561 tgctattata aagataagaa ctgctctgga atgacggttt aggataagtt ataatcattt
     1621 tagaatatgt ttggcaggaa tggctgtcat aagaggagaa gggagaatat catgaattat
     1681 ctcttgggaa tagatgtggg aacttccgca acaaagacgg tcctgtttga tgaaaaggga
     1741 accgtgatcg cttctgcttc acaggaatat ccactgtatc agccggaaaa tggctgggca
     1801 gagcagaagc ccgaggactg gagagatgca gttcttaaga ccataaagga agttgtttca
     1861 cagtcaggtg tagacaaaga cgcagtaaag ggaattggta tttccggaca gatgcatggc
     1921 ctggtcatgt tagatgaggc cggagaggtg atccgtcctt ctattatctg gtgcgaccag
     1981 agaacaggcg cagaagtaga ggacatgtta aagatcatgc caagggaaga atggatccgt
     2041 atcacagcta acccgccact gacaggctgg actgcagcaa agattctctg ggtacggaaa
     2101 aatgagccgg aaaattataa ccgctgccgt catatcttac tgccgaagga ttacatccgc
     2161 tacatactta ccggtgtatt tgcaactgaa gtttctgatg caagcggtat gcagctttta
     2221 gacgttccgg gccgctgctg gtctgatgaa gtgctgaaag cattaaatat tgacaagaac
     2281 cttttaggaa aggtacatga gtcctgcgaa gcaacaggaa cactgcttcc ggatgtagca
     2341 aaggaattag gcctttctga aaaatgtatc gtagccggcg gtgccggtga taatgctgca
     2401 gctgcagtgg gaaccggtgt tgtaaaagac ggaactgcct ttactaccat tggtacttcc
     2461 ggtgttgtat ttgcacatac cagcaagcct gccattgatc cgaagggaag gatccatacc
     2521 tgctgctgcg cagtaccggg tgcatggcat atgatgggtg tgacccaggc tgcaggactt
     2581 tccttaaaat ggtttaaaga ccagttctgc caggattatg taaaagaggc agaggccaag
     2641 ggagtagatg tatatgacca gatcaatcag gatgttgcgt ctgtagcacc tggaagtgac
     2701 cgtctgctgt atcttcctta tttaatggga gaaaggaccc cgcatctgga tccggactgc
     2761 agaggcgtat ttttcggtct gtctgcgatc cacacaagaa agcatctgct gcgggctgta
     2821 atggaaggcg ttgcttattc tctctgcgac tgcaatgaga tccttcagga aatgggcgtt
     2881 aaggttgact gtatgaaagc ctgcggaggc ggcggaaaga gccctgtatg gcgccagatg
     2941 ttagcggatc tgtacaattg ctccgtagag cgtgtggtac aggatgaagg tccggcactt
     3001 ggcgttgcca ttttagcagg agttgcctgc ggtctttatg aaagtgtgga aaaagcatgt
     3061 gaccagttga tcacacgcag ggacagcaca gagccaaagg cagaagatgt ggctgtttat
     3121 gaaaaatatc acagactgta tcagaaattg tatacaagcc tgaaaaatga ttataaggag
     3181 ctggcagaat tatcatgatc aaacagataa gtgtaatgga gccggaattt aaaaagtacg
     3241 gcaaggtcat cggaggttat gacctgaaag aaattttaca gaaaatggaa gaaactccat
     3301 gcccggatga tgtagtatat gtggcttctg taaaggaact ggaagattta aagatagcag
     3361 aagaactggc agacggtgtt tacggacaga tgccgatcca gatcggttac tgcaacggac
     3421 acaaccgtaa attaaatgcg ttagagtatc acagagactc tgagatcaat attgcagtca
     3481 gcgatatggt actgctttta gcaaaagaag aggaagtaga ggatgactat acattagata
     3541 cctctaaggt aaaagcattt ttcgttcctg caggcaccat gatcgaagtg tatgcaacga
     3601 ctcttcacta tgcgccatgc cagacctctg ataaaggttt ccgctgtgta gttgtccttc
     3661 cagagggaac taacggggag ttaagccaca agccggaaaa taagagcggc gaagagaagc
     3721 ttttggcagc tgtcaataag tggctgatcg gtcatgcaga aggcggactt ccggaaggca
     3781 gctttattgg actgaaaggt gaaaatacag aagtttagag cataaaaaag tataaataag
     3841 ggctgtggtg gaacagccct gtaaatatgg aggaaaaaac atgtttgaga atatcccgaa
     3901 agtaaaagtt ggtatcgttg cagtcagccg tgactgtttc ccggagtctt tatctgtaaa
     3961 ccgcagaaaa gccctggtag acgcttacaa ggcaaaatat gatccagagg acatttatga
     4021 gtgcccagtc tgtatcgtag agagcgagat ccacatggta caggccttag aagatattaa
     4081 gaaagaggga tgcaacgctt tagttgttta ccttggaaac ttcggacctg aaatttctga
     4141 gacccttctt gcaaagcact ttgacggacc gaaaatgttt gttgcagcag ctgaagagac
     4201 cggaaacaac ctggtatccg gcagaggcga tgcatactgc ggtatgttaa atgcaagcta
     4261 taacttacag ctgagaaata tcaaggctta tatcccggaa tatccggtag gaaccgctgc
     4321 agagtgtgca gatatgatcc atgatttcct gccaatcgca agaactgtga tcggtttgtc
     4381 tgatctgaag atcatcagct ttggcccaag accattaaac ttcttagcat gcaacgctcc
     4441 tatcaagcag ctgtacaacc tgggtgtaga gattgaagaa aactctgagt tggacctgtt
     4501 tgaggcattt aataagcatg caggtgatcc aagaattgca gaagtagcgg cagacatgga
     4561 agcagagtta ggagcaggca ataagaagcc ggagatcctt gcaaagctgg ctcagtatga
     4621 gattactctg ttagactgga tccgtgacca cagaggctac cgcaagtatg ttgcgatcgc
     4681 aggaaaatgc tggcctgcat tccagaccca gtttggcttt gttccatgct atgtaaacag
     4741 ccgtctgact ggccgtggca tcccggtatc ctgtgaagta gatatttacg gtgcattaag
     4801 tgaatttatc ggaacctgca tcagccagga tgcagtaacc cttctggata tcaacaactc
     4861 tgtaccggaa gatctgtaca atgaggacat taagggcaag tttaactatg acttaaagga
     4921 aaccttcatg ggcttccact gcggaaatac caattccaag aagctggcat tctgtgaaat
     4981 gaagtatcag ttcatcatgg cacgttctct gccggaagaa gttacacagg gaactctgga
     5041 aggcgatatc atgcctggtg atattacgtt ttatcgttta cagagtacag ctgacaacat
     5101 tctgcgcgct tatgtagcac agggcgaggt actgccagtg gcaacccggt catttggttc
     5161 catcggcatc tttgcaattc cacagatggg acgtttctac cgtcatgtgc tgatcgaaaa
     5221 gggcttccca catcacggag ctgttgcttt cggtcacttt ggaaagagcc tgtatgaggt
     5281 atttaagtac atcggtgtag atgtagagga gatcggcttt aaccagccgg ctggaatgct
     5341 gtacaagaca gaaaatccat ttgcataatt tcacataaaa atttatcccc tttctgtggc
     5401 ggttctgtta cagagagggg attttttgcg ttgcaggaaa ctttgcagta catttgtgat
     5461 accggtgtca cacttgcatc agggcggttg ttcggctata ctagctgtaa attatgatgt
     5521 tggggtcaaa aggtattttg ccccctctga taccgaattg ctacgtgctt tgcactctcg
     5581 caattctaaa aacattcgta attcgctcat ttttcttcaa aaaatggcta cttactcatg
     5641 tttgttgcgg gtcccaagtt caaaaatcct cctgcaagca agcttgcgtc ggatttcaga
     5701 ccttttgacc ctggtatcaa attatgtgat tatttacaaa cgtatgaggt tactaccatg
     5761 aaatatcaga taaaggacgg caccgttacc ttaggcggtg aaaccatatt atcccatatc
     5821 gattttgaga tacagggaaa ccagaaaatt gcagtagtgg gaagaaatgg cgcgggaaag
     5881 actacattac tgcgtctgat cgcaggggag ctgagtctgg accgggatga caggcgccag
     5941 ggaccgggga tcacagcttc caggcagctg acagtggaaa tgttaggaca gcaggcactg
     6001 gcaggggaag aaaggaccgt agaagagctg gtgatgttaa actgccctgc aaagggacct
     6061 tttgacaggg aatgttttga atatgagcgg gaatatgaca ccctttttac aggtctggga
     6121 tttcagaaag aagataaaaa acgttctgta gcagcttttt ccggaggtca gaagaccaag
     6181 atcgccctga tccagcttct tcttaaaaag ccggatctgc tgcttcttga tgagcccact
     6241 aatcatctgg atatggaaac agccagctgg ctggaaggtt atttaaggca gtatccgggg
     6301 gctgtggtca tggtcagcca cgaccgtttc tttatggacc ggactgcaga tatcgtatat
     6361 gaactggaac agggaaaatt gacccgttat ccgggaaatt atacgaatta ccgggaacag
     6421 aagcgaaaaa actatgagat ccagatgaaa tcctatctgc gccagcagga ggagatcgag
     6481 cgtcaggaag aactgatcaa acggtttaaa aataagcctt ccaaggctgc atttgcccgt
     6541 tccagaaaga aaatattaga acggatgctt ccggtggaaa agccaaggga ggatatggcc
     6601 catattttta cagggactat taccccgctg atcccaggaa gcaaatgggt ctttgaggca
     6661 gaacatttaa agatcggcta tgacaaacat tcattactgg aactgagcct gcgtatccgg
     6721 aaaggacaga agattggtat tttaggtgta aatggttccg gcaagactac ttttttaaag
     6781 acagtagccg gatttttacc tgctgtagcc ggggaatgtg cattgggaaa taatattact
     6841 atcggctatt ttgaccagta ttccgctgcg atccagtcag aaaaaacagt agtagaacat
     6901 ttttcggatt tgtttccgtc cctgacggac aaggaagtgc ggacgatttt aggggcctat
     6961 ctttttaaag gaaaggacgg ggcaaaacgg gtggatgatc tgtccggagg tgaaaaagca
     7021 aggctggtgc tggcagaact gctgcaaagc cggccaaact tcctgatctt agatgagcct
     7081 accaaccata tggatatcca ggcgaaagag accttagagt ccgcctttca ggcctatgaa
     7141 ggcaccatcc tttttgtatc ccatgaccgg tattttatcc gtcaggtggc aaagtccgtg
     7201 ctcatttttg aaaataacgc tgctttttat tatccttttg gatatgagca ttatctggaa
     7261 cggaaagaaa aagaggcttc cggagagcca ttggcagcca ggataaaggc agaagagcag
     7321 gcgctgatcg aaggcttaaa agctgtgcca agggcagaac gtcacaggct gcgggagatc
     7381 ccggaggaag aagcttatga ccagtggaga ctgcggctgg cagcagaggc gatagagaat
     7441 gctgcagagc agacggaaga gatctggcag cagattttgg aaaaaaatgg aaaaagagaa
     7501 gaatgggagc tggagcactg ggatcagtgg ttaaatgaaa ttgctgataa gaacgaggat
     7561 gcggcttgtg gtgaaaagca ggaaaaacag gcagaagagg aactttctgc tttcacagag
     7621 caatacgaaa atgcccagtc tgcctggacc gatgcctgtc ttttctggta tgacatctgg
     7681 tgtgaggctc atccggaacc agtcatggat cctggaacat cagacacaga tggatctgtt
     7741 ccgcaagata cagagtcgtc caaaacggac ggctccgaaa tataagtttg atttactgcg
     7801 tgttcaccag cccccccagt tctgtgatct cacagccgcc acgggtcctg tgaactttga
     7861 tatatccggc actggccagt cgctgcaggc tggtgcggat attggcttca gaaagctgga
     7921 tcccttcttc tttcagcctg ctgcacagga aagaacgtcc gctgcgggga ttggcagcaa
     7981 taagggacag gatccggtgt tccgttatat ctaactgctg ttcatgcttt cggatctggt
     8041 ttaaaatgta tcccggcagg tcgcttaatg acaaaggttc tctgtgatag atacaggaaa
     8101 agtaggtaca aacattttta agttccagca catttcccgg ccagtcatac tggtttaaga
     8161 aggttaatac cggttctgtc agcagttcac gggaagaaag ccccggttca tggaaagagc
     8221 gtttcaggaa ggtttccaac agcaggggga tatcatttcg cctctgcctt aaaggaatgg
     8281 tatataaaga tacggtactt aaaaggaaaa acagttcatc caggaatttc ccttccagta
     8341 cacgggaata caggtcactg acagatgtga cgatcaggcg cagctccagt ggctggggag
     8401 aggaaccatt tcccaaaggg ataaaaaagc cattttttaa gatacttaaa agcaggcgct
     8461 gcagccccgg gtccgcatac tgcaggttat tgatcagcag ggttccgccc cgtgctgctt
     8521 ccagtacgcc gatcttttta tgtaccccgt tttcattctt ctcatagctc ccgatcaggg
     8581 ctgcttccgc agactctgat tttaaggaag aaatatttaa ggaaataaaa ggcccgttgt
     8641 gacgtcggga gttctgatgg atggcctgtg ccagcaattc tttttctgtt ccgctttctc
     8701 cttctatgat aatcggaaaa tcattcacag agacccttct ggcagtgtct aacatatgca
     8761 gctgcagcgg gtttacagta gaaaagtaac tgaaatcgca taatttcgta atacgggtag
     8821 gaaactgagc atcctggagc gggtttgtat ggatcaggcg cacctgacgg ctgtctgcaa
     8881 tgctgaagtc ctgaaaccac atgcggaaag cttcctctcc attgtgtaca gcaattccgt
     8941 cattctggct gggataatcg taaatgatcc cggcctcttt taacaatctt gaaaaatctt
     9001 tttcctgaat atcccgttct ttgatcccgg tcattttcag aaaaagggaa ttacacagct
     9061 ctattttgcc ggactggttt agcaggcaca ggccttctga ggcattctcc aaaattcccc
     9121 gggtgatctc tttcatatcc agagcctggc ttaactgctg gttggaaagt ttctgtatct
     9181 ggataatatg gtttaagtaa ttttttgtaa cctcatcagc cagactggcg ggaagtttaa
     9241 atacggcgat cagttcattg atggtggaaa tgtctacaat acggttgccg atatctacta
     9301 cctggcggat aaaagaaggg actaagtgta cctctccggg agttacacaa tactggatat
     9361 ctttccgtac ctctccagta cccggaagat aggggagaaa atggtactgg gaaaagccat
     9421 attcttttaa ctgttccatg atctcatagg tggtgtccac tgtatcattg accaggtata
     9481 caaaagaccc aggcgggatc tgaaggatgc gctggagata ggtatggtta aatgtacgca
     9541 tacataccag ctgttttaca gaatccggaa tggaaaaagg catctgctct tttaattcac
     9601 ctgctgtata caggatacag cctgcatctt ccatatcctc cggcttcagg tcagcaattc
     9661 tggtagacac aatggtaagg tattgtccaa aaagggaaga aaggtaagag gcgatcccta
     9721 cttttgccac cggattccgg gtcaggatca atagtttatg cttcatgtaa gtaatcctcc
     9781 ttattaggtt gattatatat cgataaaaca aataaaacaa cctaaagaca ataaaaaata
     9841 aattttaata agaaaagtgt tgtattttct gtacggacat ttgacagcga tataaagaat
     9901 cgtatagaat ggtcactaaa gcaagggcgc tttgaaaaca gtcagggaac tgtgcatgcg
     9961 agaagtgtgc cctgcttttt ctatatagtc ttcccgcaag gaaggctggc ggaaaaagta
    10021 agatagagga ggatatgaaa aatgaagaaa agagctttat tagtagcact tgcagcagca
    10081 atggcagcaa gccttgcagg ctgcggcgga tcttctgcat ccagcaccac agcagctggc
    10141 agcgcagcag aaagcagtga agcagctgga aacacagcag cagacgcttc tggaaaggac
    10201 agcctggtta tcgtaacatc atctgatgta ctgtctatgg atccatacag atatgatgag
    10261 ggacctacaa accagatcat gctccacatc tatgaggcaa tggttgccca gaatgcagat
    10321 atgtcctttg gtccttgtct ggcagaaagc tgggagacct ctgaggatgg tttaacctgg
    10381 accttccatt taagagaagg cgttaagttc catgatggtg atgtaatgga ttctggcgac
    10441 gttgttgcat ctatcaatct ggcttccaat cctgattccc catctgcata cagcagctac
    10501 acatccagct ttgagtctgt ggaagcacct gatgcaaata ccgttgttat taagactgaa
    10561 aaaccaaacc ctttaatgct gtttgacgtt gcccagctgt atgtattaaa acaggaaatt
    10621 gcagaagttg gcacagaaga gcaggttgca gacaatgtaa ttggtaccgg ccgttacaaa
    10681 ttcgtagagc aggttaagga agaccatatt gacctggcag caaacgaaga ttactggggc
    10741 gaagttccgg ctattaagaa tgtacgtttc cgtcctatta caaatgaagc aaccagaacc
    10801 gctaccatgc tgactggcga tgttgatttt actattgacg tatctgcaag agatattgac
    10861 cgtctggatg caacggaggg tatcagcgtg atcaagcaga agggtcttcg tgagatctac
    10921 ctgaacctgg acagccgtga ggattctcct ctgttccctg gacagaaaaa tccaatgtct
    10981 gatgtcaaag taagacaggc tatgtatctg gcaattgatg aaggaaccat tatcaagaac
    11041 atcatgaatg gatgcgcata tgaaatgaac tctgttgttc cggaagatta tgtaggctac
    11101 accgaagtta cccgtgaagc atatgatcct gaaaaggcaa aacagctgtt agcagaagct
    11161 ggatatccag atggttttga agtaacctta gatgctccaa atgaccgtta catgaacgat
    11221 gagcagatcg cccaggcagt agcaggttat cttgaaaaag taggtatcaa ggttaattta
    11281 aacctgatgc caaaggctaa cttcttcagc tacattaaac cattagagaa taaatccatg
    11341 ttcttaatga ccggatggtc tgatgcttct ggtgatggac tttctcttat gcacgatatg
    11401 ctttacacct ataaccgtga ggcaggtgat ggcggtgtca acagaggcca ttactccaac
    11461 gcacaggtag acgccctgat cgatcaggca ttcactgaga tggatgatac caagagaggt
    11521 gaactggtag cagaggctga caagatcgca agagaagatt atgcatacat cccgcttcac
    11581 tttgagcagg atacatatgc gatcaaggat actttaaatt acaccccaag aatgaacaaa
    11641 tatgtatatg catgggagtt ctcttacaaa taaaagttag gtaacaaagt aaaggggccg
    11701 tgtaaaatgg aacggctcct tttcattaaa atgtactctc ggagtctttc atgcacttgc
    11761 ttttgtggct gattttccgt cagtcgcacc cgaattttat caacgtacgc accgcgtacg
    11821 cctcaaaaat ttgggcacaa ctgacaaaaa atcttctcac aaaatca
//
LOCUS       DBMDUA010000076        11665 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2793_length_11665_cov_2.611985, whole genome shotgun sequence.
ACCESSION   DBMDUA010000076 DBMDUA010000000
VERSION     DBMDUA010000076.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11665)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11665)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11665
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2793_length_11665_cov_2.611985"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(15..1715)
                     /locus_tag="ACZPCL_07340"
                     /pseudo
     CDS             complement(15..1715)
                     /locus_tag="ACZPCL_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594441.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
     gene            complement(2297..2563)
                     /locus_tag="ACZPCL_07345"
     CDS             complement(2297..2563)
                     /locus_tag="ACZPCL_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1162314.1"
                     /translation="MISKNLTVVNPSGLHLRPAGVLSQTAMKFKSDITIICGEKKIVA
                     KSVLNVMAAGIKCGTEITLVCDGEDEEEAMKTISQAIESGLGEM"
     gene            complement(2720..3376)
                     /locus_tag="ACZPCL_07350"
     CDS             complement(2720..3376)
                     /locus_tag="ACZPCL_07350"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIR1162315.1"
                     /translation="MAAQQQKENYQRVLDQTIEQLTKEGKVPTLLLHSCCAPCSSYVL
                     EYLSKYFKITLLYYNPNISLKEEYEARVREQKRLIGEMDFVHPVQFMEGKYLPEEFYR
                     AVKGHEEDKEGGERCFICYEQRLREGAIVAKAGGFDYFTTTLSISPLKNAQKLNEIGI
                     RLGEEYGVPYLKSDFKKKNGYKRSVELSAQHHLYRQNYCGCVFSKREAELREQQSKEQ
                     "
     gene            complement(3627..4769)
                     /gene="rpoD"
                     /locus_tag="ACZPCL_07355"
     CDS             complement(3627..4769)
                     /gene="rpoD"
                     /locus_tag="ACZPCL_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR1162316.1"
                     /translation="MEKDDFLKKLEKLVAHAKSKHNTVDAGEINDFFVGDNLSPEQMD
                     QIYSYLENRNIDVVPVLDEAMLNADPALDDIGLDLDLDDDSFMKDAEDEDIDLDAVDL
                     LEGIGTEDPVRMYLKEIGTVPLLTADEELELAQRKADGDEAAKERLIEANLRLVVSIA
                     KRYTGRGMSFLDLVQEGNLGLIKGVEKFDYTKGYKLSTYATWWIRQSVTRALADQART
                     IRVPVHMVETINKMSKMQRKLTLELGYEPSVAELAEALDMTEDKVMEIMQIAREPASL
                     ETPIGEEDDSNLGDFVADSNVVTPEGNVESVMLREHIDALLGDLKERERQVIVLRFGL
                     EDGHPRTLEEVGKEFNVTRERIRQIEAKALRKLRNPVRSKRIRDFL"
     gene            complement(4887..5783)
                     /locus_tag="ACZPCL_07360"
     CDS             complement(4887..5783)
                     /locus_tag="ACZPCL_07360"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR1162317.1"
                     /translation="MKKMLFLFNPRSGKEQIKSHLLRILDIFSKAGYDIHVHVTQCQL
                     DARKTVEHLGKNVDVIVCSGGDGTLNETISGMLQLKNTPSLGYIPAGSTNDFASSLKI
                     SKNMEKAAREIVNGFPVPVDAGMFCGDRSFIYIAAFGAFTEVSYQTPQDRKNLLGHQA
                     YMIESVKSLASIKPYHMRVEWDDHVLEEDFVFGMVTNTRSVGGFKGLVNQSVSLNDGV
                     FEVLLIRMPKTPLDLSNIISYMFLVEEPNEYVYKFKASAIRFTSEESVDWVLDGEYGG
                     ARTEVTIENLRERIPFLLPYKM"
     gene            complement(5825..7009)
                     /gene="mutY"
                     /locus_tag="ACZPCL_07365"
     CDS             complement(5825..7009)
                     /gene="mutY"
                     /locus_tag="ACZPCL_07365"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR1162318.1"
                     /translation="MCKEKQYGGSLHFYDDLQILEREDEPLSRKERLQAVEKPLLSWY
                     HSRARILPWREDPKPYKVWISEIMLQQTRVEAVKPYFERFMAAFPDVASLAEAQDDHL
                     MKMWEGLGYYNRARNLKAAAIQIMDQFDGCIPSSKEELLTLPGIGSYTAGAISSIAYG
                     VPNPAVDGNVLRVISRLQGDYSDIKKASVKTAMEQEIAAVMPKDAASSYNQGLIEIGA
                     LVCIPGGEPRCRECPLESLCITKKKGLWKEIPVKSVPKPKRMEEKTVFLVEWNEKVAI
                     HKRPPKGLLASLYELPNIEGHLTKEEAAEQVKSWIWEDDGKREEKPTGITVEPLGTAK
                     HIFSHVQWDMVGYRVKIAWENEKEPGKLLENTHFFMVDRAALKEEYSVPSAFGAYVRL
                     LG"
     gene            complement(7002..7982)
                     /locus_tag="ACZPCL_07370"
     CDS             complement(7002..7982)
                     /locus_tag="ACZPCL_07370"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR1162319.1"
                     /translation="MKAEILKILKKEKDYVSGQELCESLGVSRTAVWKAIRQLEEQGY
                     VIEAVRNKGYRLVEEADVLTVAELHSVLDTKWLGKELEYYYETDSTNNRARDAAEKGA
                     SHGFLAVADCQTAGKGRRGRVWNSPHGTDIYMSFVLRPTFTPSQASMLTLVAGMAVVK
                     GVQKATGLSAMIKWPNDAVVNGKKICGILTEMSTEEDAIRYVVPGIGINVNATEFPEE
                     IRDKATSLKLELGRSVKRSDVICAVAESFEEYYEIFEKTCDMSGLLSAYDEMLVNMEK
                     TVCVLDPKGEYRGKALGIDEEGCLLVEKENGEVVHVLSGEVSVRGIYGYV"
     gene            complement(8023..8325)
                     /locus_tag="ACZPCL_07375"
     CDS             complement(8023..8325)
                     /locus_tag="ACZPCL_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6637 family protein"
                     /protein_id="HIR1162320.1"
                     /translation="MQGQRGRNERRAGVFLDVAHLILGIAIVIFAVLSIINPEGNEML
                     FPLVFLLAALLNGINGVFELKTCGRDKKRFRMGAFQMVLCAGLGLLGVLSALSLWF"
     gene            complement(8385..9374)
                     /gene="argF"
                     /locus_tag="ACZPCL_07380"
     CDS             complement(8385..9374)
                     /gene="argF"
                     /locus_tag="ACZPCL_07380"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR1162321.1"
                     /translation="MNLEGRNFLTLKDYTEEEITYLLDLAAELKEKKKKGILVDTLKG
                     KNVALIFEKTSTRTRCAFEVAAHDLGMGSTYLDPSGSQIGKKESIADTARVLGRMYEG
                     IEYRGFGQEIVEELAKYAGVPVWNGLTNEDHPTQMLADLLTIREHLGHLKGVKLVYMG
                     DARYNMGNSLMIACAKMGMHFVACAPKKYFPDEALVKECQEFAAISGATITLTEDVAE
                     AVKGADVVDTDVWVSMGEPDEVWTERIKDLTPYKVTKEVMDAAGPQAIFLHCLPSFHD
                     LKTKIGKEMGERFGVSELEVTDEVFESSQSVVFDEAENRMHTIKAVMLATLSE"
     gene            complement(9450..10610)
                     /locus_tag="ACZPCL_07385"
     CDS             complement(9450..10610)
                     /locus_tag="ACZPCL_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR1162322.1"
                     /translation="MELKLDTNKEYGLVLEGGGAKGAYQIGAWKALKEAGIHVKGIAG
                     TSVGALNGALIAMDDFEKAERIWETIRYSRVMDVDDELVEQLKTSGLKDIAALGLSEL
                     IPAAKKVLKDRGFDIAPLRSLIEEVVDEEKIRNSEKELYVVTYSLSDRKPMVVNVKEV
                     PDGEIADMLMASAYLIGFRREKLGGKYYMDGGGVNNVPIDVLIEKGYKDILVFRIYGY
                     GVDTERRLKVPDDVHLYHVAPRQDLGGLLEFDRRRARKNMVLGYFDAKRMLYGLEGRV
                     FYLDAPESEIYYFNRLLAEAPELLADIWTQLSETELFTAQMASCRRYTEEWFPKLAKA
                     LHLKEDWDYRELYLSLLEHLARQYKISRFKIYTPQELLLIIQRKRKRIFLDR"
     gene            complement(10585..10887)
                     /locus_tag="ACZPCL_07390"
     CDS             complement(10585..10887)
                     /locus_tag="ACZPCL_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR1162323.1"
                     /translation="MTGICGWFLAFLFFLGAECICRCLVSLWFACGAVAAFVLAALGR
                     QIEEQLLMFVLASLAAFLLIRPLSFLGNFLKNLKTGDFPAKTKKKVGGHGAEAGYK"
     gene            complement(10918..11664)
                     /locus_tag="ACZPCL_07395"
     CDS             complement(10918..11664)
                     /locus_tag="ACZPCL_07395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR1162324.1"
                     /translation="MNLRDKAKARREEGLYVAEGLRMCREYKPDDVEILYITENFGKQ
                     EENKKWLRGFKFEFVTDEVMHYMADTKTPQGVLAVAKQKKCTLEDILKKTKTQKLLMI
                     LENIQDPGNLGTIIRAGEGAGITGVVMSSDTADIYNPKVIRSTMGSVLRVPFVYASDL
                     KEACEQMKKAGISLYAAHLKGMHNYDQEDYTKDTGFLIGNEAKGLTDEMAAVADTYIK
                     IPMAGQVESLNAAVASSVLMFEAARQRRNS"
BASE COUNT         2873 a         3033 c         2183 g         3576 t
ORIGIN      
        1 gaaggctatt cgcccttaca ttcgtccgtt tcaggatttt ctacacggag gatcttgcag
       61 ttttccggtg tctcctccag gatattgccc tcttcaccta catatacagt agtggaagta
      121 acgatctcct cacggatggc atcgatcggc gggttggtaa cctgtgcaaa taactgctta
      181 aagtagttaa ataatggctg atgcttcttg gaaagcactg ctaacggaga gtcaacaccc
      241 atagctgaaa cagcttctgc accgtttaat gccattggaa ggatcatggt gcggtactgc
      301 tcgtaagtat agccataagt cttctgtaag cggagacgct cttcttttgt aagtgtagga
      361 gctgcctggt tcggtacttt cagatcatct aattctacca ggttggagtc cagccattcg
      421 ccatatggat ttttctctgc atagcgcagc tttaattcct catcgcttac catttttcct
      481 tctactgtat ctaccagaag catttttcca ggacgaaggc ggtctttttt aataaccttt
      541 gtctggtcaa tatcaatggc accaacctcg gaagaaagga tcatatagcc gtcatctgta
      601 atatagtaac gggatggacg caggccattt cggtcaagga ttgcacctac cacatcacca
      661 tctgtaaaca ggatggatgc aggtccgtcc cacggctcca tcatagttgc atagtagcgg
      721 tagaagtcct tgatctcgga gtccatgtat ttatcatgtt cccatggagc aggaatagtt
      781 accataacag ccagcggcag gtccatacca ctcatgacca taaattccag ggcattgtcc
      841 agcatagctg agtcagaacc ttcctgatta ataataggaa ggactttgtg catatcgtac
      901 ttaaaatact cagactccat gttctcttca cgggccagca tcttatctac gttgccgcgg
      961 atggtgttga tctcgccgtt gtgaacgata aaacggtaag gatgagctct catccagctt
     1021 ggattggtgt tggtactgaa acgggaatgt acgatggcaa tggcagactc ataatcctta
     1081 tcctgaaggt ccgcaaagaa cagacgcagc tcgcttacca ggaacatacc tttgtataca
     1141 atggtcctgc tgcttaagga tactacatag gtatcttcat tactctgttc aaatacacgg
     1201 cggactacat acagctttct gtcaaagtcc aatcctttct ttacgttggc aggttttgca
     1261 acaaagccct ggacaatgta aggcataacg tctactgctt ttttgcctaa tacatctggt
     1321 gttgtaggaa catctctcca tccaaggaag gtaagtccct ctttcttgac gatgatctca
     1381 aacatcttca tggcctgttt tctggcaagt tcatcctgtg ggaagaagaa cataccaacg
     1441 ccgtattctc tctcaccgcc taaggtaata ccagcctgtg ctgctgcttt cttaaagaaa
     1501 cggtgggaga tctgaagcat gataccgaca ccgtcaccgg tctttccctc tgcgtcttta
     1561 cctgctctgt gctctaagtg ttctacaatg tggagtgcct ggtcaactgt tttatgtgtt
     1621 ttgatgcctt tgatattggc aactgcaccg ataccgcagt tatcatgctc aaaacgaggg
     1681 tcatacagtc ccggcagttt ctgtttggga tccatagtgt tcttcctttc tttacgtgcc
     1741 tcaggaaatg ggatcttcct ctaagcttat gtcacaggaa agaacgatat ggggggatgt
     1801 tctttcacag ttggggtaag acgtataagc atcagcactt ttttattgag cagataatac
     1861 tactattttt ctcatttgta aacaattgat tttcgtgata ttgtcagata attagattat
     1921 ttaatgttgt tattattttt gccagaatat tttgaagtgt tttccattta ttatctctat
     1981 tgttcgtgta aaatcagccc cttgacacac tttattttct taatttttca ctttctattc
     2041 ttaagcgaaa cggaggggca tcataatcgt ttacggaaag cagactgtgc acgtacaaat
     2101 aagaatgtgc cttggcacat tctcgcgccg agcgcggtcg cttgcgacaa acttcatcat
     2161 cgcgcgagtg cgcatcgttg gcaggtcgtg cctttttgtg taccagggag ttccaacgga
     2221 atttcctagt acacaaaaaa gagccacacc ttacaaggca tgacccttct tcttttattg
     2281 cattccggag taaaaattac atctcaccta agccgctttc gattgcctgg ctgatggtct
     2341 tcatagcttc ttcttcatcc tcgccatcgc aaaccagagt gatctctgtt ccgcacttga
     2401 taccagctgc cattacgttt aatacgctct ttgcaacaat cttcttttca ccgcagataa
     2461 tggtgatatc gctcttaaac ttcatagctg tctgggataa aacgcctgct ggtcttaaat
     2521 gtaaaccgga tggattaact actgttaaat tcttgctgat cataattata ctctccttat
     2581 cgcattttta tacgccggtt cccggctttc cgccgaacct tataagtaaa ttttattaca
     2641 tctgagtggt gatgtcaagt taatacagag ataatattgt taagcttata acagttatat
     2701 cagctatcct tcgctgccat tactgctctt tcgactgctg ttctctaagt tcagcttccc
     2761 gctttgagaa cacacacccg cagtaattct gcctgtacag atgatgttgg gcagacagtt
     2821 ctacagaccg cttgtatccg ttctttttct taaaatcaga cttaagataa ggcactccgt
     2881 attcttctcc taagcggata ccgatctcat ttaacttctg tgcgttcttt aaagggctga
     2941 tggaaagtgt ggtggtaaaa taatcaaagc cgcctgcttt cgctacaatt gccccttccc
     3001 taaggcgctg ttcgtagcag ataaagcagc gttctccccc ttctttgtct tcctcatggc
     3061 cttttactgc cctgtaaaac tcctctggca gatattttcc ttccatgaac tgtaccggat
     3121 gaacaaagtc catttccccg atcaggcgct tctgctccct tacacgggcc tcgtattcct
     3181 cttttaggga aatattgggg ttgtaatata ataacgtgat cttaaagtat ttagaaaggt
     3241 attccaacac atagctgctg cagggcgcac agcagctgtg aaggagaagc gtcggcacct
     3301 ttccctcttt tgtaagctgt tctatggtct gatccagtac cctctggtaa ttttctttct
     3361 gctgctgtgc tgccatctat ggcctcctgt gttctatcgc ttgttcgcct gatggcgaag
     3421 ctgtagtttt cattccctta aaatccagta aaaaaggacc gctccttaaa gccgcccttt
     3481 ttaagtgttc tatttcactg tgcccaattt cactcaggtt cacgcgctgc tgcgctccac
     3541 tgcctgctga cgcagccgct tgttttgcca tgcatgtcga aaatgcgcca gtgacgcgtt
     3601 cgaaggcttc ctgcactcat tatatcctac aggaaatcac ggatcctctt gcttcttacc
     3661 ggattacgaa gcttgcgcag tgcctttgcc tcgatctgac ggatacgctc tctggttacg
     3721 ttaaattctt ttccaacttc ttccagtgtc cttggatgtc cgtcttccag accaaaacga
     3781 agcacgatga cctgacgctc tctctctttc agatctccta aaagtgcgtc aatgtgctct
     3841 cttaacataa cagactctac atttccctcc ggagtcacta cattgctgtc agcaacgaaa
     3901 tctcccaggt tggagtcatc ttcctcacca attggtgttt caagggaagc tggctctctg
     3961 gcgatctgca tgatctccat aaccttatct tctgtcatat caagtgcttc tgccagctct
     4021 gcaacagaag gctcatagcc cagttccagt gtcagcttac gctgcatctt tgacatcttg
     4081 ttgatggtct ctaccatatg caccggtaca cggatggttc ttgcctgatc tgccagtgct
     4141 cttgttacgg actgacggat ccaccatgtt gcatatgtac ttaacttata accttttgta
     4201 taatcaaact tttcaacgcc tttgatcagt cctaagtttc cttcctgaac cagatccagg
     4261 aaactcattc cacgacctgt ataacgcttt gcaatgctga ctaccagacg caggttcgcc
     4321 tcgatcagtc tttccttggc agcttcatcc ccatctgcct tgcgctgtgc aagctccagt
     4381 tcttcgtctg ctgtaagaag aggtacagta ccgatctctt tcagatacat acggaccgga
     4441 tcttccgttc caataccctc taaaagatct actgcgtcaa gatcgatgtc ctcatcttcc
     4501 gcatctttca taaagctgtc gtcatctaag tcaagatcaa gcccgatatc atccagagca
     4561 ggatctgcat tcaacattgc ttcgtccagt acgggaacta catcgatatt acggttctcc
     4621 aggtaactgt aaatctgatc catctgctcc ggagaaaggt tatctccgac aaaaaaatcg
     4681 ttgatctcgc ctgcatccac agtattatgc ttggattttg catgcgcaac cagcttttcc
     4741 agctttttta aaaaatcgtc cttttccaat gataaacgtc ctttcgctta aaggtaaacg
     4801 tggatactcc cacaacttat cattatatac cagtttttta tttttttcaa catcaatcga
     4861 caactttttt taaagtgact gaactgttac attttatatg gaagaaggaa agggatcctc
     4921 tctctcaggt tttcaattgt aacctctgtc cttgcgccgc cgtattcccc atccagtacc
     4981 cagtctacgc tttcttctga cgtaaaacga atagcggaag ccttaaattt atagacatat
     5041 tcgtttggct cttccaccag gaacatataa gaaataatgt tgcttagatc taacggagtc
     5101 tttggcatcc ggatcagcag gacctcaaaa acaccgtcat ttaaggatac ggactgattt
     5161 accagccctt taaagccgcc tacgcttctt gtattagtca ccattccgaa tacaaaatcc
     5221 tcttctaaaa catgatcgtc ccactctacc cgcatgtgat aaggcttgat ggaagccaga
     5281 ctctttacgc tttcgatcat atacgcctga tgccctaaca gatttttccg gtcctggggc
     5341 gtctggtaag atacctccgt aaatgcgcca aatgccgcaa tataaataaa actgcggtcg
     5401 ccacaaaaca tacccgcatc caccggtacc gggaaaccat ttacgatctc ccgggctgct
     5461 ttttccatat ttttggaaat ttttaaactg gaggcaaaat cattggtaga tccggcagga
     5521 atatagccta aagaaggggt atttttcagc tgtaacattc ctgatatagt ttcatttaaa
     5581 gtaccgtctc caccgctgca gacgatcaca tctacatttt ttccaaggtg ctccactgtc
     5641 tttcttgcat ctaactggca ctgggtcaca tgtacatgaa tatcgtaccc ggcttttgaa
     5701 aaaatatcca gaattcgcag caaatgactt ttgatctgct ccttcccgga acgtggatta
     5761 aaaagaaaca gcattttttt catcctgcat ccctcgcttt cattgtgtcc attctttctg
     5821 tattttatcc aagtagtctt acgtaagctc caaaagcaga cggaacggaa tactcttcct
     5881 ttaacgctgc cctgtcaacc ataaaaaaat gcgtgttttc taaaagtttc cctggttctt
     5941 tttcattttc ccaggcgatc tttactctgt accccaccat atcccactgt acatgggaaa
     6001 agatatgttt tgctgtcccc aaaggttcca ctgtaatgcc ggtgggtttc tcctcccgtt
     6061 ttccatcatc ttcccagatc cagcttttta cctgctctgc cgcttcctct tttgtcagat
     6121 gcccttctat attgggaagt tcataaaggg aagccagaag gccctttggc ggccgtttgt
     6181 ggatagctac tttttcattc cattctacta aaaagaccgt tttttcttcc attcttttag
     6241 gcttgggaac cgattttaca ggtatctcct tccacagtcc ctttttcttt gtaatacata
     6301 aagactccag cggacattcc ctgcatcttg gctctcctcc cggaatacac accagggcac
     6361 cgatctcgat cagtccctga ttatagctgc tggctgcatc ttttggcata acagcagcta
     6421 tttcctgttc catggcagtt tttacagacg ccttttttat atcagaatag tcaccctgta
     6481 atcttgagat cacacggagc acatttccat ccactgccgg attgggaacc ccgtaggcaa
     6541 tggaagagat tgccccggct gtgtagctgc caattcccgg aagtgttaaa agttcttcct
     6601 tagaagatgg aatgcagccg tcaaactgat ccatgatctg aatggccgct gcttttaaat
     6661 ttctggcccg gttatagtaa ccaaggcctt cccacatttt cattaaatga tcatcctgcg
     6721 cctctgccaa agatgccaca tcgggaaatg ctgccataaa ccgttcaaaa taaggcttta
     6781 cagcctctac tctggtctgc tggagcatga tctctgatat ccatacttta taaggcttcg
     6841 gatcctctct ccagggcagg atacgggctc tggaatgata ccatgaaagc agcggttttt
     6901 ccactgcctg taagcgttct ttcctgctta aaggttcatc ctcccgttcc aggatctgca
     6961 gatcatcata aaaatgcaga ctgcccccat actgtttttc cttacacata tccgtagatc
     7021 cctcttacag atacttcccc tgaaagaaca tgaacgactt ccccattctc cttttctact
     7081 aaaaggcagc cttcttcatc gatcccaagg gcttttcccc gatattcacc ctttggatct
     7141 aaaacgcaga ctgtcttttc catattgact aacatttcat cgtatgcaga aagaagtcct
     7201 gacatatcac aggtcttttc aaatatctca taatattctt caaaactttc tgctactgca
     7261 cagatcacat cactgcgttt tacgcttctt cccagttcca atttcaggga agttgccttg
     7321 tcccggatct cttccggaaa ttccgttgca tttacattga taccgatccc gggcactaca
     7381 tagcggatgg catcttcctc tgtgctcatt tctgttaaaa taccacagat cttctttcca
     7441 tttactactg cgtcattagg ccatttgatc atggcactta agcccgtggc tttctgtacc
     7501 ccttttacca ctgccattcc cgctaccagc gtcagcatag acgcctggga tggggtaaat
     7561 gtaggacgca ggacgaaact catataaata tctgttccat gaggggaatt ccacactctt
     7621 cccctgcggc cctttcctgc tgtctggcag tctgccactg caagaaagcc gtgggaagca
     7681 cctttttccg ctgcatccct tgcccggttg ttggtggaat ctgtttcata ataatattcc
     7741 agctcttttc caagccactt tgtatcaagg accgaatgaa gctctgccac tgtaagcaca
     7801 tctgcttcct ccacaaggcg gtagcctttg ttgcgcactg cctcgatcac atagccctgt
     7861 tcttccagct gccggatggc tttccataca gcagtcctgg aaactcccag gctttcacaa
     7921 agttcctgtc ctgatacata atctttttct ttttttaata tctttaaaat ctccgctttc
     7981 attctctttc tgatcctgtt ctttaaaatc gctgaaaatc gtctaaaacc ataaactgag
     8041 agcgcttaac acgccaagaa gcccaaggcc tgcacagagt accatctgaa acgcccccat
     8101 gcggaaacgt ttcttgtccc tgccacaagt cttaagctcg aagactccgt ttattccatt
     8161 taacaaagct gccagtaaaa ataccagagg gaaaagcatt tcatttccct ccggattaat
     8221 aatagacagc actgcaaata ttacgatcgc gattcccagg atcaagtgcg ccacatctaa
     8281 aaacacaccg gcgcgacgct catttcttcc tctctggccc tgcatatctc aatctcctgt
     8341 tttccaggat gcttctttcg gctttttgcg ccctattgct cagatcattc gcttaaagta
     8401 gctaacataa ctgccttgat ggtatgcatc ctgttctcag cctcatcaaa tacaacagac
     8461 tggctggatt caaatacttc atctgttact tccagttctg atacaccaaa acgttctccc
     8521 atttccttgc cgatcttggt ctttaagtca tggaaggatg gcaggcagtg aaggaaaata
     8581 gcctgaggac cagctgcatc cattacttct tttgttacct tgtaaggagt caggtcttta
     8641 attctctcgg tccatacttc atctggctca cccatggata cccatacgtc tgtatcaaca
     8701 acatcagctc ccttaacagc ctctgccaca tcctcagtaa gagtaatggt agcgccgctg
     8761 atggctgcaa attcctggca ttcttttact aatgcttcat ctgggaaata cttctttggt
     8821 gcgcaggcaa caaagtgcat acccatcttt gcgcaggcga tcatcaggga gttacccata
     8881 ttataacgcg catcgcccat atacacaagc ttaactccct taaggtgtcc taaatgctct
     8941 ctgatggtta aaagatctgc taacatctgg gttggatgat cttcgttggt cagaccgttc
     9001 catacaggta cgcctgcata tttagccagt tcttctacga tctcctggcc aaaacctctg
     9061 tattcgattc cctcatacat acggcctaat actcttgctg tatcagcgat gctctctttc
     9121 tttccgatct gggaacctga tggatccaga taggtagaac ccattccaag gtcatgtgca
     9181 gctacttcaa aggcacatct tgttctggta ctggtctttt caaagatcag agctacattt
     9241 tttcccttta aggtatctac taaaatgcct tttttctttt tttcctttaa ttctgctgca
     9301 aggtcaagca ggtatgtgat ctcttcttct gtataatctt tcagtgttaa aaaatttctt
     9361 ccctctaaat tcataatcgt ttcctctttt gaaatatttt aaaaatttat atttatgcaa
     9421 cttttatgca taatatacca cttgtgtttt tacctgtcaa gaaaaatccg ctttcttttt
     9481 ctctgtatga tcagaagcag ttcctggggt gtgtatattt taaagcggct gatcttatac
     9541 tgccttgcca gatgctccag caaacttaaa tataattccc tgtaatccca gtcctctttt
     9601 aagtgtaatg cctttgccag cttgggaaac cactcctctg tatatctgcg gcaggaagcc
     9661 atctgggctg taaaaagttc tgtttcggaa agctgcgtcc agatatctgc caacagttcc
     9721 ggcgcctctg ccaaaagcct gttaaaatag tagatctcgc tttccggggc atccagataa
     9781 aaaactcttc cttccagtcc ataaagcatc cgtttggcat caaaataacc aagaaccatg
     9841 tttttccttg cccgtcttct gtcaaattcc aaaagaccgc ccaagtcctg tctgggggct
     9901 acgtgataaa gatgcacatc atccggtact ttcaggcggc gctccgtatc aacaccatat
     9961 ccatagatgc gaaataccag gatgtccttg tatccctttt ctattaaaac atcaatagga
    10021 acattattca ccccaccgcc gtccatataa tatttgccgc ccagcttttc ccgacgaaag
    10081 ccgatgagat acgcacttgc catcaacata tctgcgatct cgccatccgg cacttctttt
    10141 acattgacta ccatgggctt tctgtcactt aaggagtaag taactacata cagctctttt
    10201 tcagagtttc gtattttttc ctcatcaacc acttcttcaa tgaggctgcg caggggtgca
    10261 atatcaaaac ctctgtcctt taataccttt ttagcagcag ggatcagttc tgacagcccc
    10321 agggccgcaa tatcctttaa accggaggtc tttaactgtt ccaccagttc atcatctaca
    10381 tccataactc tggaatagcg gatggtctcc catattcttt ccgccttttc aaagtcatcc
    10441 atggctatga gagcgccatt taaggctcct acagaagttc ctgcgatccc ttttacatgg
    10501 atccccgctt cttttaacgc tttccatgca ccgatctggt aagccccctt ggcgccaccg
    10561 ccttccagta caagaccata ttccttattt gtatccagct tcagctccat gtccgcccac
    10621 cttctttttt gtttttgcag gaaaatcccc tgtttttaag tttttcaaaa aatttcccag
    10681 aaacgacaac ggccttataa gcagaaatgc tgccagggaa gccaggacaa acataagaag
    10741 ctgttcctct atctgtcttc ccaaagcagc cagaacaaag gcagctactg ccccgcaagc
    10801 aaaccacaaa gacaccaggc accggcagat acattctgca cctaaaaaga agagaaatgc
    10861 aagaaaccag ccacaaatac ctgtcatttt cctgcactcc cttctttaag atgtactcta
    10921 tgaattccgt ctctgtcttg cggcctcaaa cattaaaaca gaagaagcca ctgctgcgtt
    10981 taaagactct acctggcctg ccatcgggat cttgatgtag gtatcggcaa cagccgccat
    11041 ctcatcggtc agccccttgg cttcatttcc gatcaggaac cctgtatcct tagtatagtc
    11101 ttcctggtca taattatgca tgccttttaa atgagctgca tacagactga tccctgcttt
    11161 tttcatctgc tcacaggctt ctttcagatc agaggcatac acaaatggta cacgcagcac
    11221 agatcccata gtagaacgga tcacctttgg attgtagata tccgctgtat cgctgctcat
    11281 gaccacaccg gtaatacctg cgccttctcc agcgcggata atggtaccca gatttcccgg
    11341 atcctggata ttttccagga tcattaaaag tttctgtgtc tttgtcttct ttaagatatc
    11401 ttccagggta cactttttct gctttgcaac tgctaaaacg ccctgcgggg tctttgtatc
    11461 tgccatataa tgcattactt catctgtaac aaactcaaac ttaaagcccc gcagccattt
    11521 tttattttct tcctgttttc caaagttttc tgtaatatat aagatctcca catcatccgg
    11581 cttatattcc cggcacatgc gcagtccttc cgctacatat aagccttctt ccctgcgggc
    11641 tttggcctta tccctcaggt tgatg
//
LOCUS       DBMDUA010000077        11622 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2805_length_11622_cov_3.643955, whole genome shotgun sequence.
ACCESSION   DBMDUA010000077 DBMDUA010000000
VERSION     DBMDUA010000077.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11622)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11622)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11622
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2805_length_11622_cov_3.643955"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="ACZPCL_07400"
     CDS             <1..349
                     /locus_tag="ACZPCL_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162325.1"
                     /translation="LGYDSIEDMRTQEHEIISSDDNDNTVAEGYWYPDGDRSSSLYFM
                     VQRDTIYWYEFNPGQGDVQVGEPDGIVLKLGNKRRLQSGQTFQYTTSRGLDMSYSKIC
                     FEGDTTEYFWRER"
     gene            884..1513
                     /locus_tag="ACZPCL_07405"
     CDS             884..1513
                     /locus_tag="ACZPCL_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1162326.1"
                     /translation="MAESTKERILRIALELFARNGYLETSMNDIAGQLGFTKAALYKH
                     YASKQEILDKIVERMNKMDYERAEVYEMPETEPDGFAEAYLHTPIQKIRTYSLAQFDH
                     WTKEPFSSNFRKMLTLEQYRDSKLAQLHHDYLAGGPLEYMAAIFRKLADSDEDAMQLA
                     LEFYGPMYLLYSVYDGAEKKESASSLLATHIDHFIAKVESDYRKKGRTT"
     gene            1510..1704
                     /locus_tag="ACZPCL_07410"
     CDS             1510..1704
                     /locus_tag="ACZPCL_07410"
                     /inference="COORDINATES: protein motif:HMM:NF025568.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HIR1162327.1"
                     /translation="MKDVGEEKRWVLCPWCGAKTRLQILRKTELVTFPLFCPKCRHES
                     IINAKNFIIETKQPDAKTQC"
     gene            1756..2262
                     /locus_tag="ACZPCL_07415"
     CDS             1756..2262
                     /locus_tag="ACZPCL_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162328.1"
                     /translation="MDAIIYTTNTGSTGQYAKLLAQETGLPAYSLAEAKKRVFAGAEV
                     VYLGWIMAGSVKGCAGAEKRYQVCAVCGVGMGQTGTQTDSVRKKSEIPANIPLFTLQG
                     NFNVKKLHGIYRFMMEIMVKTVGKGLAAKKDRTPEEDDMLDMMLHGGERVKAENLSAI
                     LDWYRAQQ"
     gene            2339..2833
                     /locus_tag="ACZPCL_07420"
     CDS             2339..2833
                     /locus_tag="ACZPCL_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1162329.1"
                     /translation="MKNLIIYVHGKGGSAGEAEYYKMFFPNSEVIGFDYCSQTLWEAK
                     KEFFAFFTTQRSRFEHITLVANSIGAFFGISSLDETLVDRAYLISPIVDMEKLICNMM
                     QWSGVTEQELALTSIETVSAFAKQHHAGLTVMPGGEHWFHTEEQMQFLDHWIRECNAK
                     NVCC"
     gene            2872..3498
                     /locus_tag="ACZPCL_07425"
     CDS             2872..3498
                     /locus_tag="ACZPCL_07425"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005346824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1162330.1"
                     /translation="MSEKNIIIRLETKDDYRAVENLTRESFWNVYQPGCMEHYVLHCY
                     RDDPAFVPDLDFVMELGGEMIGQIIYVRSEIDCDDGRKVPIMTFGPIGVAPAYKRQGY
                     GKQLLDYSMEKAKEMGAGALAITGNIDFYGKSGFVPAKTKGVCYADDPDADYFLIREL
                     TPGFLDGVSGTYKDPEGYFVCEKDPDGFERFEATFPVKEKQKLPGQLF"
     gene            3554..3766
                     /locus_tag="ACZPCL_07430"
     CDS             3554..3766
                     /locus_tag="ACZPCL_07430"
                     /inference="COORDINATES: protein motif:HMM:NF016210.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HIR1162331.1"
                     /translation="MLDVPGYGKLPVTLIDCANPVVIFKAEDAGLNGTEICIGACSVF
                     IMLQKYLLLCWEGVRECFRSTPHQAQ"
     gene            complement(3738..4469)
                     /gene="ymfI"
                     /locus_tag="ACZPCL_07435"
     CDS             complement(3738..4469)
                     /gene="ymfI"
                     /locus_tag="ACZPCL_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P 5-aminopentanone reductase"
                     /protein_id="HIR1162332.1"
                     /translation="MSRKTVLVTGASRGIGKAIAIKFARKGYNVVISCIRNEDRLMQT
                     KKEIENYQVPCLAYMGDMGDMDCCKELFAKIKKQFGGIDVLVNNAGVSYIGLLQDMSS
                     EDWDKILHMNLTSVFNCCKLAIPYMVHQKQGKIINISSVWGVAGASCEAAYSATKGGI
                     NALTRALAKELGPSNIQVNAIACGAIDTEMNQWMEEDELIALVDEIPAGRLGKAEEVA
                     DLAYHLGYKESYLTGQIIGLDGGWI"
     gene            4713..5648
                     /gene="trxB"
                     /locus_tag="ACZPCL_07440"
     CDS             4713..5648
                     /gene="trxB"
                     /locus_tag="ACZPCL_07440"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR1162333.1"
                     /translation="MTSHIYDLIIIGSGPAGLAAAVYAQRACLDTLIIERSTVSGGQI
                     LNTYEVDNYPGYPGISGYELGMKLREHADHLGAQFKEDTVLKIEDKGLENLKQVIGMN
                     ENYEARTLILATGAVHKKLGIPGEKELTGAGVSYCATCDGAFFRNRTVAVIGGGDVAV
                     EDAIFLSRMCSKVYLIHRRGKLRAAKSLQNQLFSKGNVEILWNTEAEAIKGDGAVESL
                     LLQDHVTGEKKEISLQGVFIAVGIQPETAAFEGVVDMEQGYIKAGEEGITSVPGIYAA
                     GDVRTKKLRQIVTAAADGANAVTSAEQYLSDLKRG"
     gene            5657..7285
                     /locus_tag="ACZPCL_07445"
     CDS             5657..7285
                     /locus_tag="ACZPCL_07445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR1162334.1"
                     /translation="MNTWKKLLIISGMCSLAGVTPAFASTNLETGSSLAGISVALNNY
                     YAGNAQPEKKLASSYNQLEATETKKSSETKTATETKAAATAKAETKAQTSSGAKTETK
                     AAASTEVKAAAVSQTKAASSAYDNIAVSKVSGSVNIRTEANTSSSITGKIYNDCAATI
                     LDTVDGEGGKWYKIQSGSVTGYIKSEYFVTGQKAETKAKEVGKTYGTVVDTTSLRLRE
                     SPDLTSKTLTLLSEGAQYSVVGQEGDFLKVAVDTDLEGYVFKDYMKTSVEFNKAVSVE
                     EEKAKEEDEAKRKEEADKAMKELEAAKKAESKAAETKAETKAETKAETTAAKKETTAA
                     PKEETKKEKTKETEAAIKVIAANPEKAEDVTVAAPTVKQETKAETKAETKAETKAETV
                     AETKTETKSEGPGAKKETSSSKKTESSAVETASRTALIAYAQQFLGNPYVYGGTSLTK
                     GADCSGFVMSVFSHFGISTGRSSRDQAARGKAIPVSEVKPGDLLFYASGSYINHVGIY
                     VGNGKIIHSSTPATGICYAPSNYRTPCKAVTFFD"
     gene            complement(7400..7933)
                     /gene="hpf"
                     /locus_tag="ACZPCL_07450"
     CDS             complement(7400..7933)
                     /gene="hpf"
                     /locus_tag="ACZPCL_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR1162335.1"
                     /translation="MRYTITGRNIEVTPGLKAAVEKKIGKLEHFFTPDTEVIVALSAQ
                     KDQQKIEVTIPVKGNTIRAEESSTDMYVSIDLVEEIIERQIRRYKKKLIDKKQAAIAF
                     SQAFIEDEEDTAYEDDIQIVKTKKFAMKPVNPEEACLQMEMLGHNFFVFLNSETEQVN
                     VVYKRKNGTYGLIEPEF"
     gene            8535..11105
                     /gene="secA"
                     /locus_tag="ACZPCL_07455"
     CDS             8535..11105
                     /gene="secA"
                     /locus_tag="ACZPCL_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR1162336.1"
                     /translation="MNVIEKLFGTHSERELKMIRPILAKIENLRPEMIAKSDEELRDQ
                     TRILKAKLADGATLDDILPEAFATVREAARRTLNMEHFPVQLIGGIVLHQGRIAEMRT
                     GEGKTLVSTCPAYLNALKGKGVQIVTVNDYLAKRDAEWMGQVHRFLGLTVGVVLNDMN
                     SAQRKEAYACDITYVTNNELGFDYLRDNMSIYKEQLVLRDLDYCIIDEVDSVLIDEAR
                     TPLIISGQSGKSTKLYEVCDVLARQLERGTVSKEFSKIDAIMGEEIEETGDFVVDEKD
                     KVVNLTEQGVKKVEEYFHIDNLADPENLEIQHNIILALRANNLMFRDKDYVVKDDEVL
                     IVDEFTGRIMPGRRYSDGLHQAIEAKEHVNVRRESRTLATITFQNFFNKFAKKAGMTG
                     TALTEEKEFRNIYGMDVIVIPTNRPVIRKDLDDAVYKTKKEKYNAVVEEVVKAHEKGQ
                     PVLVGTITIETSELLSKMLTKKGIPHKVLNAKFHELEAEIVADAGIHGSVTIATNMAG
                     RGTDIKLDQESKDLGGLKIIGTERHESRRIDNQLRGRSGRQGDPGESRFYLSLEDDLL
                     RLFGSERLMHMFEAMGVPEGEQIEHKMLSNAIEKAQMKIEGNNYGIREQLLKFDEVNN
                     EQREVIYKERRKVLDGDNMRDLVLKMITDIVENAVDMSVSDEDTAEKWDLTELNNLLL
                     PIIPLKSVVLTDEQKKSMKKNELKHTLKEAAIKLYETKEAEFPEPEQIREIERVVLLK
                     VIDNKWMSHLDDMDALREGIGLQAYGQRDPVVEYKMQGYELYEAMMAAIQEETVRILF
                     HIRVEQKIERESAAKVTGTNKDATPIAPKKRDEQKIYPNDPCPCGSGKKYKQCCGRIK
                     "
     gene            11210..11582
                     /locus_tag="ACZPCL_07460"
                     /pseudo
     CDS             11210..11582
                     /locus_tag="ACZPCL_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271338.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
BASE COUNT         3551 a         2266 c         2871 g         2934 t
ORIGIN      
        1 actgggttat gactccattg aggatatgcg cacacaggaa catgaaatta tctcatccga
       61 tgataacgac aatacagtcg ccgagggata ttggtatcct gacggggata ggagcagctc
      121 cctctatttt atggtccaga gagataccat ttactggtat gaatttaacc cagggcaggg
      181 ggatgttcaa gtgggagaac cggatggcat tgtactcaag ttaggaaata agcgccgtct
      241 tcagagcggg cagacatttc agtacacaac ttccagagga ttggatatga gctacagcaa
      301 aatttgtttc gagggagaca caacagaata tttttggagg gagcgctgaa aggcattcct
      361 gtggttaaag aagaaagcta tatggaacaa agatatattt caaaagtaaa gccactaagc
      421 cagtatattt tacaagttga ctttgtctcc gacagtcgtt tgctcctgga tatgaagctc
      481 ctgtgtcttt gtccctgaag gcactgttcc cggctgggca gttgatacgg aaaccggtaa
      541 tggtgagttt tcaaacatcg ctggtatctg gtatctggat ggagatgcgg atgcaattac
      601 aaaggtgttg atccaaagac ggtgcagtat ccggcaggcc acgagaacat caaaaccctt
      661 ggatatccag tattttcaag ggttttctta tgtttatggt tcataaagaa ttgatgtgaa
      721 atttggtcaa attcttctta atgttatcaa aaattgattg aatgcctggg gataaacggg
      781 aatacaggat tgaaaatata tttttttcat tagttcttga caagtaacca ttcggtaact
      841 ataatggagt taccgaatgg ttactttgat aaaggagata gttatggcag agagtacaaa
      901 ggaacggatt ttgaggatag cactggagct gtttgcacgg aacggctatc ttgagacgtc
      961 tatgaatgat attgcaggac agttaggatt tacgaaagcc gccttatata agcactatgc
     1021 cagcaaacaa gaaatcttag ataagatcgt ggagcggatg aacaagatgg actatgagcg
     1081 cgcggaagtc tatgaaatgc cggaaacgga accagacggc tttgcggaag catatctgca
     1141 tacaccaatt cagaaaatcc gcacatacag tcttgcccag tttgaccatt ggacaaagga
     1201 accgttttct tccaatttcc gtaaaatgct gacgctggag caataccgtg attccaagct
     1261 tgcacagctc catcatgact atctggcggg tggtccactg gagtacatgg cggccatctt
     1321 ccgcaagctg gcagattctg atgaggacgc tatgcagttg gcgttggaat tctatggccc
     1381 aatgtatctt ttatacagtg tctacgacgg tgctgaaaaa aaagaatctg cttcttcttt
     1441 gctggcgaca catattgatc actttattgc caaggttgag tctgattaca gaaagaaggg
     1501 gagaacaaca tgaaagatgt gggagaagaa aaacgctggg tcttatgccc gtggtgcggt
     1561 gccaagacgc gcttgcagat actgcggaag acggaattag taacttttcc tctgttttgt
     1621 ccaaaatgca ggcatgagag tattatcaat gcaaaaaatt ttatcatcga aaccaaacag
     1681 ccagacgcta agacgcagtg ctgatcgcaa aaacgatcat gcgctgcgtc ctattttttg
     1741 aaaggagcaa ctattatgga tgcaattatt tacaccacta acaccggaag taccgggcag
     1801 tatgcaaagc tgctggccca ggaaaccgga cttccggcat attcccttgc agaagcgaaa
     1861 aaacgggtct ttgctggtgc ggaggttgtc tatcttggct ggatcatggc cggttcggtc
     1921 aagggttgtg ccggggctga gaagcgctat caggtgtgcg ctgtctgcgg cgtcggcatg
     1981 ggacaaaccg gaacacagac agacagcgta cggaaaaagt cagaaattcc agccaacatt
     2041 ccgctgttca ccctgcaagg aaacttcaat gttaaaaagc tacacggtat ttatcgattc
     2101 atgatggaga tcatggttaa aacagtgggc aaaggtctgg ccgcaaagaa agaccgcacg
     2161 ccggaggagg acgatatgct ggatatgatg ctccacggcg gtgagcgggt caaggcggaa
     2221 aatctaagcg ccattctcga ttggtacaga gcgcagcaat aaatgttctc tcggggtctt
     2281 tcttacacct gcttttgtgg atgattttcc gctgtcttag aaaggggaat gagataaaat
     2341 gaaaaattta attatatatg ttcacggaaa gggcggttcg gctggggaag ctgagtatta
     2401 caaaatgttc tttcctaata gtgaagtaat cgggtttgat tactgttcgc aaacgctttg
     2461 ggaagccaag aaagagtttt ttgcattttt taccacacag agaagtaggt ttgagcacat
     2521 taccttggtt gcaaacagca tcggggcttt ctttgggata tcctcgctgg atgaaacgct
     2581 ggttgaccgg gcttatttga tctcgccaat cgtggatatg gaaaaactga tctgcaatat
     2641 gatgcaatgg tcgggggtga ccgaacagga acttgcctta acatccattg aaacggtatc
     2701 tgcatttgcc aagcagcacc atgctggcct gaccgtcatg cccggcggcg agcactggtt
     2761 ccatactgag gaacagatgc agttccttga tcattggatc agagaatgta atgcgaaaaa
     2821 tgtctgctgt taattataca aaatcattga taaaagagaa ggagaataat tatgtcagaa
     2881 aagaatatta tcattcgtct ggaaacaaaa gacgattaca gagccgtcga aaacctgacc
     2941 cgcgagtcat tctggaatgt ttatcagcct ggctgtatgg agcactatgt gctgcattgt
     3001 tatagggatg acccggcatt tgtgccggat ctggattttg tgatggaact gggcggtgag
     3061 atgatcgggc agatcatcta tgtacggtcg gagatcgact gtgacgatgg acgaaaggtg
     3121 ccgatcatga ccttcggccc cattggcgtt gcacccgcat acaagcggca gggctacgga
     3181 aagcagcttc tcgactattc catggaaaaa gctaaggaaa tgggtgcagg ggcacttgcc
     3241 attaccggca atattgactt ttacggcaag tctggctttg ttccggccaa aaccaagggc
     3301 gtgtgctatg ccgacgaccc ggatgcggac tattttctca tcagagaact gacccccgga
     3361 tttctggacg gcgtctccgg cacttacaaa gaccctgagg ggtattttgt ctgtgagaaa
     3421 gacccggacg gatttgaacg gtttgaagcc accttcccgg taaaagaaaa gcagaagctg
     3481 ccgggacagc tgttttgaaa atcccggtgg cggtcttaca ggaaagctgt ttccaactgg
     3541 caataaacag gatattttag atgtgcctgg atatggaaaa ctgcctgtaa ctcttattga
     3601 ctgtgccaat ccggtagtca tttttaaagc agaggatgca ggattaaatg gaacagaaat
     3661 ctgtataggc gcctgttccg tttttattat gttgcagaaa tacctcttgc tgtgctggga
     3721 gggggtgaga gaatgtttta gatccacccc ccatcaagcc caatgatctg tcctgtaaga
     3781 taggattctt tataaccaag atgataagcc agatcagcta cttcttctgc tttaccaagg
     3841 cgtcctgccg gtatctcatc taccagtgcg atcagttcgt cttcttccat ccactggttc
     3901 atttctgtgt caatggcacc gcaggcaata gcattgacct ggatgttgct tgggcccagt
     3961 tctttggcaa gagcacgggt cagagcgttg atgccgcctt tggttgcaga gtaagctgct
     4021 tcacaggaag ctcctgcaac accccataca gaggaaatat tgatgatctt tccctgtttt
     4081 tggtgtacca tataagggat tgccaatttg cagcagttaa aaacagaagt taaattcata
     4141 tggagaattt tatcccagtc ttcactgctc atatcctgaa gcagtccgat gtaggaaaca
     4201 ccggcattat ttaccagtac atctatacct ccaaactgtt tctttatctt tgcgaacagt
     4261 tctttgcagc aatccatatc tcccatatct cccatatagg caagacaagg tacctgataa
     4321 ttctcaattt ctttttttgt ctgcatcagg cggtcttcat tgcggatgca gctgatgact
     4381 acattatagc ctttccgggc aaatttaatg gcaatggctt ttccgatacc acgggaagcg
     4441 ccggtcacaa gtacagtttt tcttgacata agctcacgtt cctttctgca tgtgtacagt
     4501 actgtttctg ttcgcgtgtt ccacgatcag tgacgcagta tttgctcttt ctattttagc
     4561 acagaatgga gaggttggca tatatctggt tgatctgtaa agataattgt aagaatttga
     4621 agtgcaacaa tagtaaaatc tatgctataa taattacgaa ttggttacaa tatattaaga
     4681 gataatgaaa aaatagacag gaaacaataa aaatgacatc acatatctat gatctgatca
     4741 taatcggttc aggaccggcg gggcttgcag cagctgtgta tgcccagaga gcctgcctgg
     4801 acactctgat aatcgaacga tctacggtaa gcggcggaca gatattaaat acctatgaag
     4861 tggataatta tcccggatat cctgggatca gcggttacga actgggaatg aagctgcggg
     4921 aacatgcaga tcatctgggt gcgcaattta aagaagatac agttttaaaa atagaagata
     4981 aaggcctgga aaacttaaaa caggtcattg gaatgaatga aaactatgag gcaaggacac
     5041 tgatccttgc aacaggagcc gttcataaaa agttaggcat tcctggtgaa aaagaactga
     5101 caggagccgg tgtttcttac tgtgctacct gtgatggtgc atttttcagg aacaggaccg
     5161 ttgcagtgat cggcggcggt gatgtagcag tggaagatgc cattttcctt tcacgtatgt
     5221 gcagtaaagt ctatctgatc cacagacggg gaaaactccg tgcagcaaaa agccttcaaa
     5281 atcagctttt ctcaaaaggg aatgtggaaa tactctggaa tacagaggca gaggctataa
     5341 aaggtgatgg agcagtagaa agtttattgc tccaggatca tgttacaggt gaaaagaaag
     5401 aaatttctct tcagggcgta tttattgctg taggaataca gccggaaaca gctgcttttg
     5461 aaggcgttgt ggatatggaa cagggttata tcaaagcagg agaagaggga attacttctg
     5521 tacctggtat ttatgcagca ggcgatgtga gaacaaaaaa attgcggcag atcgttacag
     5581 cagcggcaga cggtgccaat gcagtgacca gcgcagagca gtatctgtca gacttaaaga
     5641 gaggataaga gtaccgatga acacatggaa aaaattactg atcatcagcg gaatgtgcag
     5701 ccttgcaggt gtgacaccgg cttttgcgtc cacaaatctg gaaaccggtt cctctcttgc
     5761 gggaatttcc gtagcgttaa ataattatta tgccggaaat gcacagccgg aaaagaagct
     5821 ggcgtcatct tacaatcagt tggaggcaac agaaacgaaa aaaagcagtg aaacaaagac
     5881 tgcaacagag acaaaggccg cagcaactgc aaaggcagaa acaaaagccc agacctcatc
     5941 cggagcaaaa acagagacaa aagcagcggc ttctacagaa gtaaaggcag cagctgtttc
     6001 acagacaaag gcagcttctt ctgcctatga taatattgca gtatctaaag taagcggatc
     6061 tgtaaatatc cgtacagagg caaatacctc cagttccata acaggaaaga tctacaatga
     6121 ctgtgcagca acgatcttag atacagtaga cggcgaaggc ggaaaatggt acaagatcca
     6181 gtccggctct gtaaccggct atattaaatc cgaatatttt gttacaggcc agaaagcaga
     6241 aacaaaggca aaagaagtag gaaagaccta tggaacagtg gtagatacca ccagccttcg
     6301 tttaagagaa agcccggatc tgaccagcaa gacactgaca cttttatcag aaggcgccca
     6361 atattctgtt gtgggacagg aaggtgattt cttaaaagta gctgtggata cagacctgga
     6421 aggttatgta tttaaagact acatgaaaac cagtgtagaa tttaataaag ctgtttctgt
     6481 agaggaagaa aaagccaaag aagaagacga agccaagcgt aaagaagaag ctgacaaggc
     6541 aatgaaagag ttagaagctg caaagaaagc ggagagcaag gcagcagaaa ccaaagcaga
     6601 gacgaaagcg gaaaccaagg ctgagacaac ggctgcaaag aaagaaacaa cagctgctcc
     6661 aaaggaagag accaagaagg aaaagacaaa agaaacagag gcagctataa aagtgattgc
     6721 agccaatccg gaaaaggcag aggatgtgac agtagctgct ccaactgtta aacaggagac
     6781 aaaagcagaa acaaaggcag aaacgaaagc tgagacaaag gcggaaacag tagcagaaac
     6841 aaagacagaa accaagtcag agggacctgg agcaaagaaa gagacaagct cttccaagaa
     6901 gacagaaagt tctgctgtag agacagcttc cagaactgca ctgatcgcct atgcacagca
     6961 gttcttagga aatccttatg tatacggcgg caccagtctg acaaagggcg ctgactgttc
     7021 cggctttgtt atgagtgtat tctctcattt cggtatcagt acaggacgca gttccagaga
     7081 ccaggcagca agaggaaagg caatcccggt cagtgaagta aaaccaggag accttttatt
     7141 ctatgccagc ggcagctata tcaatcatgt aggtatttat gtaggaaatg gaaagatcat
     7201 ccactccagt acacctgcta ccggtatctg ctatgcaccg tctaattaca gaacaccttg
     7261 caaggcagtt acattttttg actgatcctg ttgaaacgta aaagtacata atcaaaatta
     7321 aatatgcatc ataatgcaca taaaaatggg ggagccggtt ttggctcccc tgtttgtttt
     7381 tatgaagttt taaaagtttt tagaattcag gttcgatcag tccataagta ccatttttac
     7441 gcttgtacac tacatttacc tgctcagttt cggagttcag gaatacgaag aaattatgtc
     7501 ctaacatttc catctgaagg caagcttcct caggatttac tggcttcata gcgaatttct
     7561 tggtttttac gatctgaata tcatcttcgt aagcggtatc ttcttcatcc tcaataaatg
     7621 cctgggagaa tgcgatagct gcctgcttct tgtcgatgag tttcttttta tatctgcgga
     7681 tctggcgttc aatgatctct tctaccagat caatggatac atacatatcg gtgctgcttt
     7741 cttcagcacg gatggtgttg cctttaactg gaatggtcac ctcgattttc tgttggtctt
     7801 tctgagcgct gagcgctacg atcacttcgg tgtcaggagt gaaaaaatgt tccagctttc
     7861 cgattttctt ttcaacggct gctttcaaac ctggagttac ctcaatattt cgtcctgtta
     7921 tggtataacg cataatcatc aactcctttt tctgagatat gcacattata tcatgtttat
     7981 tataaaatta caacataaaa tctcaaattg actaataatt tatcttaatt ttttataaaa
     8041 aaagtatatg tagggtgcaa atggctattg aattattaat agtgcacatg aaaaaatgtc
     8101 aatagtaact gtgcatattt tgacgaaaaa cagcagattt atgagatata tacaaaaaag
     8161 aaaggacagg aaaatgtaaa aggggtgtaa aaaagaacag ggtaaaatat gtggataatg
     8221 tggataagtc ggtgtataac tagaaaatgc agaaaaaccg ataaaaatcg atgtggataa
     8281 ctttggggga aaactttgtg gataagtgtg gacaatgtgg atgtgcacaa aatcgaacat
     8341 actttttgtg caagatgtct ggtttaccat aatatggggg gaaatgtaaa ctttttatga
     8401 agtcgttgaa taaatcccgc tcaagtgtta caatgtatta gattgtatca taagcgtact
     8461 ggctgcattt gcggcacaga tgcaggttga aaagaatttt catttatata aggaagaagt
     8521 taggagaact taccatgaac gtaattgaga aactgttcgg gactcacagt gaaagggaat
     8581 tgaagatgat ccgcccgatt ttggcgaaga tcgaaaacct gcgccccgaa atgattgcca
     8641 agtcagatga agagctgcgc gatcagaccc gtatcctgaa agcaaagctg gctgacggag
     8701 caactctgga tgatattctg ccagaggcat ttgctactgt ccgtgaggcg gcaagaagaa
     8761 cactgaatat ggagcatttc ccggtacagc tgatcggtgg tatcgtactc catcagggcc
     8821 gtatcgcaga gatgcgtaca ggtgaaggta aaaccctggt atctacctgt ccggcttacc
     8881 tgaatgcatt aaagggaaaa ggcgtacaga tcgttactgt caacgattac ctggcaaaac
     8941 gagatgctga gtggatggga caggtacatc gtttcctggg tctgactgta ggtgttgtat
     9001 taaacgacat gaacagtgct cagagaaaag aagcatatgc ctgcgacatt acctatgtaa
     9061 caaacaatga acttggtttc gactacctgc gtgataatat gtccatttac aaagaacagc
     9121 tggttctgcg tgatctggat tactgtatca tcgatgaggt tgactctgtt ctcatcgatg
     9181 aggcaagaac acctctgatc atttccggac agagcggcaa gtctaccaag ctgtatgaag
     9241 tatgcgacgt tttagcgcgt cagttagagc gcggtactgt ttcaaaggaa ttctccaaga
     9301 tcgatgccat catgggtgaa gagatcgaag agacaggcga tttcgtagta gatgagaagg
     9361 ataaagtagt taacctgact gaacagggcg tcaagaaggt agaggaatac ttccacattg
     9421 ataacctggc tgatccggaa aatctggaga tccagcacaa tatcatcctt gcacttcgtg
     9481 ccaacaacct gatgttccgc gataaggact atgtagtaaa agatgacgaa gtcctgatcg
     9541 ttgatgaatt tacaggacgt attatgccag gccgccgtta ttctgatggt ctgcatcagg
     9601 cgatcgaagc aaaagagcat gtaaatgtac gtcgtgaaag ccgtacactg gctaccatta
     9661 ctttccagaa cttcttcaat aagttcgcaa agaaggcagg tatgaccggt accgctctta
     9721 cagaagagaa ggaattccgt aacatctatg gaatggacgt tatcgtgatt ccaaccaacc
     9781 gtcctgttat ccgtaaggac ttagatgacg ctgtttataa gaccaagaaa gaaaaatata
     9841 atgcagttgt tgaagaagtt gtaaaggcac atgagaaggg acagccggtt ctggttggta
     9901 ccattaccat tgagacttcc gaacttctca gcaagatgct gaccaagaag ggcattcctc
     9961 ataaagtcct gaatgcaaag ttccatgaat tagaggctga gatcgttgca gatgccggta
    10021 tccatggttc cgttaccatc gctaccaaca tggcaggccg tggtaccgat attaagctgg
    10081 atcaggagtc aaaggattta ggcggcttaa agatcattgg tacagagcgt catgagtccc
    10141 gtcgtattga caaccagctg cgtggacgtt ccggacgtca gggagaccca ggtgagtcca
    10201 gattttacct gtccctggaa gatgacctgt taagactgtt cggttctgag cgtctgatgc
    10261 atatgtttga ggcaatgggc gtaccggaag gtgagcagat cgagcataag atgctttcca
    10321 atgctattga gaaggctcag atgaagatcg agggcaataa ctacggtatc cgtgagcagc
    10381 tgcttaaatt tgacgaagta aacaatgagc agcgtgaagt tatctacaaa gagcgccgca
    10441 aggttctgga tggggataac atgcgtgatc tggttctgaa gatgatcact gacattgtag
    10501 agaatgcagt agatatgtct gtttctgatg aagatacagc agagaaatgg gatctcactg
    10561 agttaaacaa tctccttctg ccgatcattc cattaaagtc tgttgtactg acagatgagc
    10621 agaagaagtc catgaagaag aacgaattaa agcacacctt aaaggaagct gccatcaagc
    10681 tgtatgagac aaaggaagct gaattccctg agccagagca gatccgtgag atcgagcgtg
    10741 tggttcttct gaaggttatt gacaacaagt ggatgtcaca tctggatgat atggatgctc
    10801 tccgtgaagg aattggctta caggcatatg gccagagaga tccggttgtt gagtacaaga
    10861 tgcagggcta tgagctgtac gaggctatga tggctgcgat ccaggaagaa actgtacgta
    10921 tcctgttcca catccgtgta gagcagaaga tcgagagaga gtctgctgcc aaggttacag
    10981 gtaccaacaa ggatgcaact ccgatcgctc caaagaaacg tgatgagcag aagatctatc
    11041 caaacgatcc atgcccatgt ggttccggaa agaaatataa acagtgctgc ggacgtatta
    11101 aataagtcct atatgaacga attgaaaaat cttcctgcgt ggcagtaaac catgcagggg
    11161 gatttttgca aaataattaa aaatttataa taagaaagcg ggtgaagctg tggttgagtt
    11221 agacaatttt aaatatacgt tatcaacgta tgacaaacca ttagtggaag tgagggattc
    11281 actttgacct ggataataag gtcagacgaa tcgacgaatt agataaatcc atggaagagc
    11341 cgggattttg ggataatccg gagcgctcta ccaaactggt gcgggaagca aaacacttaa
    11401 aagacgaagt tgatactttc cgtgaactgg agcaggaata tgaagacatc cagaccatga
    11461 tccagatggg ttatgaggaa aatgatgctt ctctgatccc tgagatccag gaaatgttag
    11521 atcatttttc agagaccctg gaaaagatgc gtatgaagct tctgctttcc ggcgaatacg
    11581 atggctatga agaacgagga agataaactc tccgcctgtt tg
//
LOCUS       DBMDUA010000078        11561 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2816_length_11561_cov_3.215009, whole genome shotgun sequence.
ACCESSION   DBMDUA010000078 DBMDUA010000000
VERSION     DBMDUA010000078.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11561)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11561)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11561
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2816_length_11561_cov_3.215009"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /gene="mscL"
                     /locus_tag="ACZPCL_07465"
     CDS             complement(<1..411)
                     /gene="mscL"
                     /locus_tag="ACZPCL_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009429535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR1162337.1"
                     /translation="MKKFLEEFKAFALKGNMIDLAVGMIIGSAFTSIVNSLVNDIFMP
                     VLSIFTGKIDFSNMFIALDGNKYPTLAAANEVTATINYGSFITQAINFLLMAFVVFMV
                     IKAINKLEKIGKASEAPKAPTEKECPYCKSMIPIK"
     gene            613..975
                     /locus_tag="ACZPCL_07470"
     CDS             613..975
                     /locus_tag="ACZPCL_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1162338.1"
                     /translation="MSFELDRIEELCNDRGWSHYRLAKEMDISPNSIGNLFRRTTVPS
                     IPTLRHICQVFGISMGEFYSTDGSQTILNKQQKYVLDLYNQLGPSNKIRAESYLEGLA
                     DGSYRKKRNKKEKEKDSR"
     gene            complement(1202..3334)
                     /gene="nrdD"
                     /locus_tag="ACZPCL_07475"
     CDS             complement(1202..3334)
                     /gene="nrdD"
                     /locus_tag="ACZPCL_07475"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIR1162339.1"
                     /translation="MDCSKIKVVKKDGTREDFNVQKVLSAISKSAERAMITFSRAERN
                     FICEFVEEKAEEMDQELIPIAQMHNIVEGALEKVNPLVAKSYRDYRNYKQDFVRMLDD
                     VYKKSQSIMYIGDKENSNTDSALVSTKRSLIFNELNKSLYQKFFMTVEELQACREGYI
                     YIHDMSARRDTMNCCLFDVKSVLTGGFEMGNLWYNEPKTLDVAFDVIGDIVLSAASQQ
                     YGGFTVPSVENILAPYAEKSYAKYLEKYRKLGLPEEKVEEVAWADVQRDMEQGFQGWE
                     YKFNSVSSSRGDYPFITMTAGTGTSRFAKMATMTMLKVRKGGQGKAGHKKPVLFPKVV
                     FLYDEKLHGPGGELEDVFEAGIDCSSKTMYPDWLSLTGEGYIASMYKKYGKIISPMGC
                     RAFLSPWYERGGMHPADDKDEPVFEGRFNIGVVSLHLPMILAKARQENRDFYEVLDYY
                     LELIRRIHIRTYAYLGEMKASTNPLAYCEGGFYGGHLGLHDKIKPLLKTATASFGITA
                     FNELQQLYNKKSLVEDGQFALEVLEHINKKIDEFKEEDGNLYAIYATPAENLCGLQVK
                     QFRKKYGIIENVSDREYVSNGFHCHVTEDITPIEKQDLEYRFWELSNGGKIQYVKYPI
                     SYNKEAIRTLVRRAMRMGFYEGVNLSLAYCDDCGHEELEMDVCPACGSKNLTKIDRMN
                     GYLSYSRVKGDTRLNESKMAEIAERVSM"
     gene            3606..4514
                     /locus_tag="ACZPCL_07480"
     CDS             3606..4514
                     /locus_tag="ACZPCL_07480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1162340.1"
                     /translation="MLITYLTATACAVAAICLGRSEYEKKALSVEEMNLYSDKVSKDR
                     TFIFLSDLHENTFGPDNEKLIRAIDKIRPDAILNGGDLIVTKGKHAKTRHALSLIKEL
                     ATRYPVYCGEGNHENRIDWEPETYGVSGSQYRKELKKAGAICLKDQSALFDHEIEITG
                     LDLDHPFYGKALFHPLPSMPSDYIEKKAGKADPDHFQILLAHSPLYFDACAKWGADLT
                     LSGHFHGGTIRLPGLGGVMTPQYQFFLPWCAGIFEKQGHYMAVSRGLGTHSINIRLNN
                     RPQLLVIRICHPKEGSCAKPTLKRIL"
     gene            4516..5313
                     /locus_tag="ACZPCL_07485"
     CDS             4516..5313
                     /locus_tag="ACZPCL_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR1162341.1"
                     /translation="METITYKLEHFEGPLDLLLHLIEKNKVDIYDIPIVEITAQYLEY
                     VRNMEREDLNLVSEFLVMAATLLDIKAKMLLPKEVDEEGEEVDPRAELVARLLEYKRY
                     KILAEELADREENAAGHFYKASSLPPEVAKYRPPTDLDELLDGLTLAKLQRIFEQAMK
                     RKEYKVDPIRSKFGTIHREPVSLEQKITSVMDYARKARRFSFRQLLEKQTDKLEVVVT
                     FLAVLELMKIGKIHLTQEETFGEMDIETIEPEGQEEDLNLEQVSDLV"
     gene            5367..6050
                     /gene="scpB"
                     /locus_tag="ACZPCL_07490"
     CDS             5367..6050
                     /gene="scpB"
                     /locus_tag="ACZPCL_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR1162342.1"
                     /translation="MEELTIDNTPVTNEKDQEAVIEAVLFTMGSAVELKQLATAIGQD
                     KETAKAAVERLMKRYQTGKHGMEILQLEDSYQMCTQSKYYSNLIRIASAPKKQVLSDI
                     VLETLSIIAYRQPVTKLEIEKIRGVKSDHAVNRLVEYNLVYEAGRLDAPGRPALFATT
                     EEFMRRFGIGTLSDLPNMDPEQEDEIRTAVEEELKFKLEELGSSNATEEAQELLEAAV
                     TKEQEQEKE"
     gene            6061..6432
                     /locus_tag="ACZPCL_07495"
     CDS             6061..6432
                     /locus_tag="ACZPCL_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1162343.1"
                     /translation="MITYQRLWETMKKRGITQYHLIKREKFSAGQIGRLKNNQTVSTQ
                     TLDTLCQLLSCRIEDIVTYIPDGGEHLPDPEPDQTSPVPSKKVTKSEKPDKSDKTEKS
                     DKKNKKKSKKKNKDKDKKQGD"
     gene            complement(6441..7217)
                     /locus_tag="ACZPCL_07500"
     CDS             complement(6441..7217)
                     /locus_tag="ACZPCL_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1162344.1"
                     /translation="MLRVMIVEDEDMIRKGLVFTIDWLSMDCVVVAEASNGQEGYERI
                     LEYKPDVVIADICMPFMDGIEMIQKASEKVKFKSILLTSYAEFDYARRAIEARVCEYL
                     LKPVDEEKLAQIMKKLGEEITSVRQVEYAMEQAELEGGNLNLEYYMKLDRSENGYVSK
                     LLEAIRTRYGEKLSIESISEEMGVSASYLSRKFKEITGQTFLDFLNKYRVQQAITLLN
                     TKEYRISEISEAVGFSDYKHFCSVFKKYTLKSPTKFVKGG"
     gene            complement(7189..8937)
                     /locus_tag="ACZPCL_07505"
     CDS             complement(7189..8937)
                     /locus_tag="ACZPCL_07505"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1162345.1"
                     /translation="MKRERYFRDELWRMFAIYAILPAALFTFFCGILFMAVLLHGRER
                     ANRDYLAYVTGRVKQTVESCEQGIETLAELPQAVSGPGYVLERNRIFQIFYGISEQAG
                     YEPELYLVDQRGRLLLSNKEKLPSYLNKRQEVEWGIYAAMDARPGQTAFRLMNGWDSG
                     ASTAVFGRWVGSEDGNGRYIILAVPGSGLLRTLARSGEQVAVTDRYGWAYFCNNGLFL
                     TDSNQVRPAVKEAGAYMRLDQKTYFTSENNLENGRFHIYVMSDVQNVLTSLVFAGILV
                     AVALLLMTAWVLFQSKQVTERKTEDLYKLLDGMELARQGNLERTGAIDRDNEFKMIAE
                     AYNETITSLKEQMENNQKMTELLALAQTKQLESQFNPHFLYNTLENIRYMCKLEPATA
                     ERMVFSLSKLLRYSLDGSREQVTLEEDLGHLNNYLSILEMRFGSRFEYKIDIAPECMQ
                     CLIPKLVLQPMIENSVKYGFGNQLNLHVELKAYIHEEKLMMICRDDGVGMSAAMVEKL
                     CALMNQKEAPGRHSGLYNIHRRIQLLYGYPYGVEIRSLEGHGTTLVVTLPAVTEKNVH
                     SVQGDNNDASGNDSGR"
     gene            complement(8934..9950)
                     /locus_tag="ACZPCL_07510"
     CDS             complement(8934..9950)
                     /locus_tag="ACZPCL_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR1162346.1"
                     /translation="MAASLSFCLFGCSGLHAVKNRGRSAEMDTDSLVVYSPHPLDFIN
                     PIVSEFESQTGIPVQVRTGGTGELLKWAKEGDEPVCDVFWGGSLYTAGAGKDIFEPYI
                     SENEEYVREEFKNKEGNMTRFTDIPSVLMVNTNLLGGMKIQGYEDLLDERLKGKIAMC
                     DPCTSSSASEHLINMLYAMGDGDPEKGWDYVRKFCENLDGVLLKSSSEVYQGVAEGRY
                     AAGLTFEEGGAGYVSKGAPVKLVYMKEGVISTPDVVCIVKGSKHKKEAEEYVDFVTGK
                     AAQTVIADSLDRRSVRKDVEAAAYLPDKEEIHMITADEALVNEKKEQWQNRFVDIFRE
                     VSGK"
     gene            complement(10029..11375)
                     /locus_tag="ACZPCL_07515"
     CDS             complement(10029..11375)
                     /locus_tag="ACZPCL_07515"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1162347.1"
                     /translation="MENYKKNTMKNGYVWISDEDNRIYLMHSVRQRSTLKHEGYLIVR
                     IKDRAFKLAEVLSQGIGLVFYDRDQNCVHVETNSEELADKIREDGLENGYQKIAGQSY
                     YIVENSIKNSEWSVIGITPISSIYHMRLKVQIVAAVLTVVTLICGCILMQYLTRKVSR
                     QINALSDTIQNAAKGEIGIQAPVYMNDDIGKIARRFNEMSLQNKKLIEELVQAEAQKN
                     SAKMEAVDYKYRFLHTQINPHFIYNSLETINAIAKVNHTPEVSRIVQLIGKYFRNITK
                     YSDHQFIALEKEFELLQCFIDIYKNIRGSNIEIRLDYPQELKSVEIPTMLLQPIVENS
                     FVHGMRGMDELFIVCLSAKGIRDEQGKLSEMILSVEDNGIGMDEEAVERLTKGKRTEP
                     DEKDRKKVFRNIGVPNIIERLKMLYGDRAKLTVTSGENGTTIQIRLPADGEKKEII"
BASE COUNT         3084 a         2861 c         2282 g         3334 t
ORIGIN      
        1 cttaatagga atcatgctct tgcaatatgg gcattctttc tctgtaggcg cttttggtgc
       61 ttccgaagcc tttccaattt tttccagttt attgatcgcc ttgatgacca taaatacaac
      121 aaaagccatc agcaagaaat tgatcgcctg ggtgataaaa gaaccataat taatggtagc
      181 tgtcacttca ttggctgctg ccagtgttgg atatttgttg ccgtctaagg caataaacat
      241 gttggaaaaa tcgatctttc cagtgaaaat acttaatacc ggcataaaaa tatcatttac
      301 aagggaatta acgattgatg taaatgcact gccaatgatc atacctacgg ccagatcgat
      361 catgttgccc tttaatgcaa acgctttgaa ttcttccaaa aactttttca tctgtcccgc
      421 ctccattttc atttacagta atgctgttgg tatcagtcta tcataatcca ggggattttt
      481 ccacttgatt ttcctgtatg tgctctgtat gcactattta atgcataatt tatacactct
      541 ttttcaagct aatgtttttt gtgttactgg gataaaatac taatataatc gtacttgaca
      601 gaaggagaag ctatgagttt tgaactggac cgcatagagg agctgtgtaa tgaccgtggt
      661 tggagccatt accgccttgc aaaggaaatg gatatttccc caaacagcat aggaaatctt
      721 ttccgccgca ccactgtgcc cagtattcct acactccgcc atatatgcca ggtattcggt
      781 atctccatgg gagagtttta cagtacagac ggcagccaga cgatcttaaa caagcaacag
      841 aaatatgtac ttgaccttta taaccagctt ggtccgtcta ataaaatccg ggcagaatct
      901 tatctggaag gattggctga cgggtcttat cggaagaaaa ggaataaaaa agagaaagaa
      961 aaagacagcc gatgattttg tcgggctgtc tttttcttgt gtacttttgt tcgtgtacgg
     1021 tccactcgca aacacgcgct agcgtgctct actgtctgct atcgcagacg gatcgttcgt
     1081 tagactcgca aacacgcgct accgcgctcc accgcctgct atcgcaggca gtttgctcgt
     1141 tgatgacgca agaaaaacaa atgccgcctt ttcggcggcg cttgtttttc ttctgcgttc
     1201 atcacatgct tactctctct gcgatctcgg ccattttgga ttcgtttagt cttgtatcgc
     1261 ctttgaccct tgaataactt aaatatccgt tcattctgtc gatcttcgtc aggtttttgc
     1321 tgccgcaggc cgggcagaca tccatttcca gttcttcgtg gccgcagtca tcacagtaag
     1381 ccagtgacag gtttacgcct tcgtaaaatc ccattctcat ggctctgcgc accagggtgc
     1441 ggatggcttc tttattgtag ctgatggggt attttacata ctggatcttg ccgccgttgc
     1501 ttaactccca gaaacggtat tccagatcct gtttttcaat cggggtgata tcttcggtta
     1561 cgtggcagtg gaagccgttg cttacatact ctctgtcaga tacattttca ataatgccgt
     1621 attttttacg gaactgcttt acctgcaggc cgcaaaggtt ctctgccggt gttgcataga
     1681 tggcatagag attgccgtct tcttctttaa actcatcgat ctttttattg atatgctcca
     1741 atacttccag tgcaaactga ccgtcttcta ctaaagactt cttattatac agctgctgca
     1801 gctcattaaa cgctgtaata ccaaaggaag cagtagcagt ttttaataat ggttttattt
     1861 tgtcgtgaag acctaaatgg ccgccgtaaa atccgccttc acagtatgcc agcggattgg
     1921 tagaagcttt catctcacct aagtatgcat aggtacggat atggatcctt ctgatcagtt
     1981 ccagatagta atctaaaacc tcataaaaat cacggttttc ctgtcttgcc tttgccagaa
     2041 tcataggaag atgaaggctg actacaccga tattaaaacg tccttcaaat acgggctcgt
     2101 ctttgtcatc tgccggatgc atgccccctc tttcatacca cggtgacagg aacgcacgac
     2161 agcccattgg agagatgatc tttccgtatt ttttgtacat gctggcaata tagccttctc
     2221 ctgtaaggga aagccagtcc ggatacatgg tcttgctgga gcagtcaata cctgcctcaa
     2281 atacatcctc taactcaccg cctggtccgt gaagcttttc atcatagaga aataccactt
     2341 tcgggaacag taccggcttt ttgtggcctg cttttccctg tccacctttt cgcactttta
     2401 acatggtcat ggtcgccatt tttgcaaagc ggctggttcc tgtacctgca gtcattgtga
     2461 taaacggata gtcaccgcgg ctggaagaaa cggagttaaa cttgtattcc catccctgaa
     2521 atccctgttc catatctctc tgtacatctg cccaggcaac ttcttctact ttttcttccg
     2581 gcaatcccaa ttttctgtac ttttcaagat attttgcata agacttttct gcataaggcg
     2641 ccaggatatt ctccacactt ggcactgtaa aaccgccata ctgctggctg gctgcactta
     2701 aaacaatatc accgatcacg tcaaaggcta catccagtgt tttcggctca ttgtaccaca
     2761 gatttcccat ttcaaaaccg ccggtaagca cagattttac atcaaagaga cagcagttca
     2821 tagtatctct tctggcagac atatcgtgaa tgtagatata accttccctg cacgcctgca
     2881 gttcttctac tgtcataaaa aacttctgat aaagggattt attcagctca ttaaagatca
     2941 gactgcgctt ggtggaaacc agagcactgt ctgtattgct gttttcctta tctccaatgt
     3001 acataatgga ctggcttttc ttgtatacat catccagcat ccgcacaaaa tcctgcttat
     3061 aattgcggta gtcccggtaa ctttttgcaa ccagcggatt gaccttttcc agcgctcctt
     3121 ctacaatgtt gtgcatctga gcgatgggga tcagttcctg gtccatctcc tctgcctttt
     3181 cttccacaaa ttcacagata aaattccgct ctgcccttga aaatgtgatc atggctctct
     3241 ctgctgactt gctaatagca gaaagcacct tctgcacatt aaaatcttcc ctggtaccgt
     3301 ctttttttac aacctttatc ttactgcagt ccattctttc acctttcctg tctgtttttt
     3361 ttgcttgtat actatatatt gtgtttcatt tatcttttat atactatata tcttttctcg
     3421 cgtatgttat cgtatcatgc ctgtttccgt ccgtcaagac ccacgttttc actattttct
     3481 tgaaaataat aactataaaa gatataatgt aaggagcaaa ccgtctgcga tagcagacag
     3541 tggagcgcga tagcgcgggg tttgtgagtt tactgagcaa aacaaaaaac gagtaaggag
     3601 acaacatgct gatcacctat ctgactgcta ccgcctgtgc agttgctgcc atatgccttg
     3661 gcagatcaga atatgaaaag aaggctctgt ctgtggagga aatgaacctt tatagtgaca
     3721 aagtatccaa agaccgtacc tttatttttc tgtccgatct ccatgaaaac acctttggac
     3781 cggacaatga aaaactgatc cgggctattg ataaaatacg gccggatgct atcttaaacg
     3841 gcggcgatct tattgtcaca aaaggaaaac atgcaaagac caggcatgcc ctttccctta
     3901 taaaagaact tgccacccgc tatcctgttt actgcgggga aggaaaccat gaaaaccgga
     3961 tcgactggga gccggaaact tatggtgtat ccggaagcca ataccggaag gaattaaaaa
     4021 aagcaggggc tatctgcctg aaagaccagt ctgccttatt tgaccatgag atagaaataa
     4081 caggactgga tctggatcat ccattttatg gaaaagcatt gttccatcct cttccttcca
     4141 tgccttctga ttacatagaa aaaaaagctg gaaaagcgga cccggatcat tttcagatcc
     4201 tgttagccca ctctccttta tatttcgatg cctgtgcaaa atggggcgct gatcttaccc
     4261 tgtcaggcca ttttcacgga gggaccatac ggcttcccgg acttggcggt gttatgaccc
     4321 ctcaatatca gtttttcctt ccctggtgcg ccgggatctt tgaaaaacag gggcattaca
     4381 tggcagtaag ccgcggtctt ggaacccatt ccatcaacat acgtttaaac aaccggccac
     4441 agcttttagt gatccgcatc tgtcacccaa aagagggaag ctgtgcaaag cccaccctga
     4501 aaaggatatt ataaaatgga aaccattacc tataaactgg agcattttga aggtcctctg
     4561 gacctgctcc ttcatttgat tgagaaaaac aaagtggata tctacgatat tcccattgta
     4621 gagatcaccg cccagtatct ggaatatgtg cgtaacatgg aacgggaaga cttaaacctc
     4681 gtcagcgaat tccttgttat ggcagccaca ctgttagata ttaaagcaaa aatgctcctt
     4741 ccaaaagagg tggatgaaga aggagaagaa gtggatcccc gtgcagagct ggtagcccgc
     4801 ctgttagaat ataaacgcta taagatactg gcagaagaac tggcagaccg tgaggaaaac
     4861 gctgccggtc atttttacaa ggcatcttct cttccgccgg aagtagctaa ataccggccg
     4921 cctacagatt tagatgaact gcttgatggt cttacccttg caaaattaca gcgtattttt
     4981 gagcaggcaa tgaagcgcaa agaatacaaa gtagacccca tccgaagcaa attcggcacc
     5041 attcaccggg aaccggtcag tctggaacag aagatcacct ctgttatgga ttatgcaaga
     5101 aaagcccgcc gtttcagttt ccgccagctt cttgagaaac agacggataa actggaagta
     5161 gtcgttacat ttttagcggt ccttgaatta atgaaaattg gaaagatcca tttaacccag
     5221 gaagaaactt tcggggaaat ggacattgag accatagaac cggaaggtca ggaagaagac
     5281 ttaaacttag agcaggtaag cgaccttgtg tagcgccgca atccccggct gccacacaac
     5341 agatatctgc cccaggagga cataaattgg aagaattaac catagacaat acacctgtaa
     5401 ccaacgaaaa agaccaggaa gccgttattg aagccgtcct gtttaccatg ggcagcgccg
     5461 tagaattaaa acagctggct actgccatcg gacaggataa agaaacagca aaagcagcgg
     5521 tggaacggct gatgaaacgc tatcagaccg gcaaacatgg catggaaatc ttacagttag
     5581 aagacagcta ccagatgtgt acccagtcaa aatactactc caacctgatc cgtattgcct
     5641 ctgcccctaa aaagcaggtg ctttctgata ttgttttaga gacactttcc atcattgcct
     5701 accgtcagcc tgtgaccaag ctggagattg agaagatccg cggcgtcaaa tcggaccacg
     5761 cagtcaaccg tctggtggaa tacaacctgg tttatgaggc aggccgtttg gatgcaccgg
     5821 gacgtccggc gctcttcgcc actacggaag aatttatgcg ccgcttcggc attggcacct
     5881 taagcgatct tccaaatatg gatccggaac aggaagacga gatccggaca gcagtggaag
     5941 aagaacttaa gtttaaatta gaagaactgg gaagttccaa tgcaaccgaa gaggcgcagg
     6001 aactgttgga agctgctgta acaaaggaac aggagcagga aaaagaatag gaggcttacc
     6061 atgatcactt accaacgttt atgggaaaca atgaaaaagc ggggcattac ccagtatcat
     6121 ctgatcaaac gtgagaaatt cagtgccgga cagatcggac gtctgaaaaa taaccagacc
     6181 gtttccaccc agactttaga taccctgtgc cagctgctct cctgccgcat tgaagatatt
     6241 gttacctaca tacccgatgg cggagaacat ttgcctgacc cagagccgga tcagaccagc
     6301 ccggttcctt ctaaaaaagt caccaaaagt gaaaagccgg ataaaagtga caaaacggaa
     6361 aaaagcgata aaaagaataa aaagaagagc aaaaagaaaa ataaagacaa agataagaaa
     6421 cagggagact gatgaatacg tcagcctccc tttacaaatt ttgtaggtga ttttaaggta
     6481 tatttcttaa atacagagca gaaatgttta taatcggaaa atcccactgc ttctgagatc
     6541 tcactgatcc ggtattcttt ggtgtttaac agggtgatcg cctgctgtac ccggtatttg
     6601 ttcagaaaat caaggaaggt ctgtcctgtg atttctttaa acttacggct taaatagctg
     6661 gcgctgactc ccatttcttc ggaaatggac tcaatactca gcttctctcc ataccgcgtc
     6721 cggatggctt ccaacagctt tgaaacataa ccattttctg aacggtccag tttcatataa
     6781 tattccaggt tcaggttgcc gccttctaac tctgcctgtt ccatggcata ttctacctga
     6841 cgcacactgg tgatctcttc tcccagtttt ttcatgatct gggccagctt ttcttcatct
     6901 accggcttta acagatactc gcagactctt gcctcaatgg cccttcttgc ataatcaaac
     6961 tccgcatagc tggtaagaag gatgctttta aattttactt tttctgatgc tttctgtatc
     7021 atttcaatac catccataaa aggcatacag atatcagcga tcaccacatc cggcttgtat
     7081 tccaggatcc tttcataccc ttcctgtcca ttggaagcct ctgccactac cacacagtcc
     7141 atggaaagcc agtctatggt aaatacaaga ccctttcgta tcatgtcctc atcttccact
     7201 atcattaccc gaagcatcgt tattatcccc ctgtactgaa tgcacatttt tttctgttac
     7261 tgccggcagt gtgactacca aagtggttcc atggccttcc aggctgcgta tttccacacc
     7321 ataaggatat ccatagagaa gctggatcct gcggtgtata ttataaagtc cggaatgtct
     7381 tcccggcgct tctttctggt tcataagagc acacagtttt tcaaccatag cagcactcat
     7441 tcccactccg tcatcccggc agatcatcat cagtttttcc tcatggatgt atgcctttaa
     7501 ctctacatga agatttaact gattgccaaa gccatatttt acagaatttt cgatcatagg
     7561 ctgcaacacc agctttggga tcagacattg catacactcc ggcgcaatat cgatcttata
     7621 ttcaaacctg cttccaaaac gcatttccag aatggacaga taattattta aatgtcccag
     7681 atcctcttcc aaagtaacct gctccctgct gccatccagg ctgtaacgca aaagcttgga
     7741 taatgaaaat accatgcgtt ctgcagttgc aggttccagt ttgcacatat accggatatt
     7801 ttccagtgta ttatagagaa aatggggatt aaactgggat tccagctgct ttgtctgggc
     7861 cagagccaga agttctgtca ttttctggtt gttctccatc tgctctttca ggctggtaat
     7921 ggtctcattg taggcttccg cgatcatctt aaattcattg tcacggtcaa tagctcctgt
     7981 ccgttccaga tttccctgtc tggcaagttc cattccatct aacagtttat acagatcttc
     8041 cgtctttcgc tctgtcacct gtttggactg gaacagtacc caggcagtca tcaaaagcag
     8101 ggccactgct accagtatac cagcaaagac caaagatgta agcacatttt gcacatcgga
     8161 cataacataa atatgaaaac ggccgttttc caggttattt tctgatgtaa aataagtctt
     8221 ctggtccagg cgcatgtaag ctcctgcttc ttttactgcc ggccgcacct gattgctgtc
     8281 tgtaaggaac aggccattat tgcaaaaata cgcccagcca tagcggtctg tcactgccac
     8341 ctgttctcct gaccgggcaa gggtccttaa aagcccgctt cccggaactg caagaatgat
     8401 ataacgtccg tttccatctt ctgaacctac ccagcgcccg aaaacagctg tacttgcccc
     8461 gctgtcccag ccattcatca gccggaaggc cgtttgtccc ggtctggcat ccatagctgc
     8521 ataaattccc cactctactt cctgtctctt gttaagataa gagggcagtt tctctttatt
     8581 cgataaaagc agccttcctc tctggtctac taaatacagt tccggttcat atcccgcctg
     8641 ttcagagatc ccataaaaga tctggaaaat gcggtttcgc tccagtacat aacctgggcc
     8701 cgatactgcc tgaggaagtt ctgccagcgt ttcaattccc tgttcacagc tttccactgt
     8761 ctgttttacc cgtccagtca cataagccag atagtcccgg ttggcccgtt ctctcccatg
     8821 aagcagaact gccataaaaa ggatcccgca gaaaaatgta aacagcgctg ctggaaggat
     8881 ggcataaatg gcaaacatcc gccataattc atcccgaaaa taacgttccc gtttcatttc
     8941 ccggacacct ccctgaaaat atctacaaac ctgttctgcc actgctcctt cttttcattg
     9001 accagtgctt catccgctgt gatcatatga atctcctctt tatccggaag ataggcagct
     9061 gcttctacat cctttcgcac agagcggcgg tccaggctgt ctgcgatcac agtctgggca
     9121 gcttttcctg taacaaaatc cacatattct tctgcctctt ttttatgttt gcttcctttt
     9181 acgatacata ccacatccgg agtagaaata actccctctt tcatgtaaac cagctttaca
     9241 ggcgcgccct ttgacacata accggcaccg ccttcttcaa aggtaagacc tgctgcgtat
     9301 cgtccctctg ccactccctg ataaacttct gaggaacttt ttaaaagcac accatccaga
     9361 ttttcacaaa actttcttac ataatcccag cctttttccg gatcaccatc tcccatagca
     9421 taaagcatgt tgatcagatg ctcactggca gatgaagaag tgcacgggtc gcacatggcg
     9481 atctttccct ttaaccgttc atctaacagg tcttcatatc cctggatctt catacctcca
     9541 agaagattgg tattgaccat aagcacactg gggatgtctg taaaacgggt catatttccc
     9601 tctttgtttt taaattcctc ccggacatat tcttcatttt cactgatata aggctcaaat
     9661 atgtcttttc ctgctcccgc tgtatataga gaaccgcccc agaacacatc acataccggc
     9721 tcgtcccctt ctttcgccca ctttaaaagt tctccggttc ccccggtacg cacctgtaca
     9781 ggaattcctg tctgggactc aaattcagat acgatcgggt taataaaatc caaaggatga
     9841 gggctgtata caaccaggct gtctgtatcc atttcagcgc ttcttccccg gtttttcaca
     9901 gcatgtaacc cactgcatcc aaaaaggcaa aaggataggg aagctgccat cactgccaga
     9961 cgtaataaat gtattctttt atttttgtga cctttttcaa acattcccta tcccctcttt
    10021 catcctattc aaatgatctc tttcttctca ccgtctgccg gcagccggat ctggatggtc
    10081 gttccatttt ctccgcttgt aacggtaagt ttcgcccggt ctccataaag cattttcaga
    10141 cgttctatga tatttggaac tccgatattg cgaaatactt tcttccggtc cttttcatcc
    10201 ggctctgtcc gttttccttt tgtcagcctt tctactgcct cttcatccat tccgatccca
    10261 ttatcttcca ctgacaggat catttctgaa agttttccct gttcatccct gatccccttt
    10321 gcagacaggc agacaataaa caattcgtcc attccccgca taccgtggac gaagctgttt
    10381 tccacgatcg gctgcagcag catagttggt atctccacac ttttcagttc ctggggatag
    10441 tccagacgga tctcaatatt gctgcctctg atatttttat aaatatcaat aaaacactgc
    10501 agcagttcaa attctttttc cagggcaata aactgatgat ctgaatattt tgtgatattg
    10561 cggaaatatt ttccaatgag ctgtacaatg cggctgactt ccggtgtatg gttcaccttt
    10621 gcaatggcat tgatagtctc cagactgtta tagataaaat ggggattgat ctgtgtatgg
    10681 agaaaacggt acttgtaatc cactgcctcc atttttgcgc tgtttttctg cgcctctgcc
    10741 tgcaccagct cttcgatcag cttcttattc tgcagggaca tttcattaaa acgtctggct
    10801 atttttccaa tgtcatcatt catatagacc ggtgcctgga tgccgatctc accttttgcc
    10861 gcattctgga tggtatctga aagggcattg atctgtcggc ttacttttct ggtaagatac
    10921 tgcattaata tacagccaca aattaaagtg actactgtaa gaactgccgc aacgatctgt
    10981 actttcaggc gcatatgata aatgctgctg atgggcgtaa taccgatcac actccattca
    11041 ctgtttttta tactgttttc tacaatataa taggattgtc cggctatctt ctgatacccg
    11101 ttttccagcc catcttcccg gatcttgtct gccagttctt cactgtttgt ctccacatgg
    11161 acgcagtttt ggtccctgtc gtaaaataca agtccgatcc cctgacttaa gacttctgcc
    11221 agtttaaaag cccggtcctt gatccgcacg atcagatatc cttcatgttt taatgtactt
    11281 ctctgcctga cactgtgcat cagataaata cggttatctt catctgagat ccatacatac
    11341 ccgtttttca tggtattttt tttataattc tccaaagcat cctgggaaaa tgtacgtatc
    11401 tcgcctttgt tttttcctat cttctcaatg gtatcacagg tatagccacc atctgtaacc
    11461 agacaaagcg taaacgcaac aatctttaca aaatcaggaa tacaagaaac taatattcca
    11521 caaacaatcg ccgtacaaaa agatagtcaa gacattacaa t
//
LOCUS       DBMDUA010000079        11523 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2823_length_11523_cov_3.264490, whole genome shotgun sequence.
ACCESSION   DBMDUA010000079 DBMDUA010000000
VERSION     DBMDUA010000079.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11523)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11523
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2823_length_11523_cov_3.264490"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(65..361)
                     /locus_tag="ACZPCL_07520"
     CDS             complement(65..361)
                     /locus_tag="ACZPCL_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1162348.1"
                     /translation="MKKERFFGKNGQTAFLGAAVLVLLICYIDNFHGGSAWIPTYFQH
                     VAVNCLIAAILALGLNFVSGYLGQTSLGHAAFYGIGAYTTAVLLTKTSHTDVVC"
     gene            complement(373..1269)
                     /locus_tag="ACZPCL_07525"
     CDS             complement(373..1269)
                     /locus_tag="ACZPCL_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1162349.1"
                     /translation="MLVFLQQVINGLAQGGIYSLIAIGWTTVFGIVGVINWTHGEIYM
                     LGAYTGYFVTTYLGLPLIPALLISMAAGALIAMAVDQFGYKPLRKKGVLAATGFITAL
                     GISTILRYTANAVFTVNPKAYPQLVDQKLITICTISGQPLTISSLHLYILIGTIIIMV
                     CLELFIKHTLTGKAMLAASQDMQTLGLMGVNAEKLIKVTFAISGALGAAAGFFIGTIY
                     AINPTMGALAGLKGWSCAILGGIGSITGSLVGGIILGIVENLTAGFISTGYKDAIGFA
                     VMILVLLIKPSGLMGYKFEEKV"
     gene            complement(1312..2544)
                     /locus_tag="ACZPCL_07530"
     CDS             complement(1312..2544)
                     /locus_tag="ACZPCL_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024850.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1162350.1"
                     /translation="MKKGYVYAMMAAVAAMTVSGCSSSSSAPASTAAESKTEAAASSE
                     SGTTAADTSDVKAAVDTIKFGLVAPLSGDNGAYGTKQEKGYELAMEEINAAGGVLGAK
                     LELETYDDGGDASTAANGAQKFADDDSILAIGGSCLTSCTAAMLPITGDAEMPQLVVS
                     SSAKSLTGISDYFFRMAVQDAAVGPQIANQFTKMGKTKAVTLYCNNDYGSGLKDSFNA
                     QFEANGGQVLDSVPYQATDQDFAAILTTVKSLDPDCIALCGTTTDGALIIKQARQMGI
                     EAPIMGQPGLYSQNVIDIAGDASEGLLCSGVFVAAGADEKGQEFVTKYGEKYSGEVPD
                     GFAALAYDQMYVLADAAERAMKENGGELTRQTLAEALKATEYEGVTGTVTFDDNGDWV
                     RDYLTLTVKDGKYVLYEE"
     gene            complement(2820..3983)
                     /locus_tag="ACZPCL_07535"
     CDS             complement(2820..3983)
                     /locus_tag="ACZPCL_07535"
                     /inference="COORDINATES: protein motif:HMM:NF019520.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HIR1162351.1"
                     /translation="MAFLVKDLVDRQIFGGVRLVAGVLNVSNPILWVNVMEILDTPRS
                     LNEGELVVSTGYGLEDQSLHKDLIHQLKKRGVSGLAIQPGYYIDQIPEYIIEDANKEG
                     LPVLELPERLSFSEILHVLMDQITENNLISRKLVQGLDTLRIALGKKLNISEKKLFEK
                     EQQVYLFLICLTGKNSRDEESLRQGMGKVGSFLSSMSKTCIGETLPDGTGMFLAPFSD
                     GEAFTDAMYDFSIFLTFLSENEEINFYVGAQPLKESRDLPDCVKEAYDHICLLDRIGA
                     KRGICTFDNRLLVELLGTLHQNEYSIVLGNQALQKLLDHDRYNHTGYVHTIRIYLAHN
                     CNATKTAEHLYIHRHTLMKRLQNISALCGINFTDYYMRVYMSLAILIHDYFTY"
     gene            4191..5174
                     /locus_tag="ACZPCL_07540"
     CDS             4191..5174
                     /locus_tag="ACZPCL_07540"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HIR1162352.1"
                     /translation="MSKKILITEAVDPAGITLLQDHGYQVVMGTGYDEETLMREAHDA
                     DGVLTRNGHFTERVLNSCPKLQVIGMHGAGVDCIDVEAAERLGIQVTNAADANSGSVA
                     EFTLGLILSLAKKLPQSRDGLRKEGWSIRPQISTFDLEGKTLGVLGMGRIGTAVARKA
                     SYGFGMKVLGYRRNLPGDICADYGILTSDLDKVIRNSDFLSIHLPYTKDTFHLVSREK
                     LELMKSDAYLLNLGRGELVDQAALRELLLSNRLAGAALDVFEGEIPDADDPLLHMDQV
                     IATPHIAGLSKQAMERLSYQAALGITEVLEGRKVTYPVNHPEKYASDVSVA"
     gene            complement(5469..8687)
                     /gene="carB"
                     /locus_tag="ACZPCL_07545"
     CDS             complement(5469..8687)
                     /gene="carB"
                     /locus_tag="ACZPCL_07545"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR1162353.1"
                     /translation="MPKNPEIKKVLVLGSGPIVIGQAAEFDYAGTQACRSLKEEGIEV
                     VLLNSNPATIMTDKDIADRVYIEPLTVEVVEQLILKEKPDSILPTLGGQAGLNLAMEL
                     EDAGFLEKHNVRLIGTTALTIKKAEDREMFKETMEKIGEPVAPSDIVEDVKHGLEVAA
                     QIGYPVVLRPAYTLGGSGGGIAQNPEQCAEILENGLRLSRVGQVLVERCIAGWKEIEY
                     EVMRDGAGNVITVCNMENLDPVGVHTGDSIVVAPSQTLGDKEYQMLRTSALNIISELG
                     ITGGCNVQYALNPDSFEYCVIEVNPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     KNAVTKKTYASFEPMLDYCVVKMPRLPFDKFISAKRALGTQMKATGEVMSICTNFEGA
                     LMKAIRSLEQHVDCLMSYDFTGLSYTGLVKELEIVDDMRIWRIAEALRRGFPYELIHE
                     ITRIDIWFIDKLAILVEMEKELKAVGEGKKELTTELLAEAKRIEFPDNVISRLTGIPQ
                     EEIKKQRYDNGIRAAYKMVDTCAAEFAAETPYYYSCFGSFNEAEKTEGRKKVLVLGSG
                     PIRIGQGIEFDFCSVHSTWAFSKEGYETIIINNNPETVSTDFDIADKLYFEPLTPEDV
                     ESIVDIEKPDGAVVQFGGQTAIKLTEALMKMGVPILGTAAEDVDAAEDRERFDEILEQ
                     CHIPRPAGHTVFTAEEAKKAANDLGYPVLVRPSYVLGGQGMQIAICDEDIDEFIGIIN
                     QIAQEHPILVDKYIMGKEIEVDAICDGTDILIPGIMQHIERTGIHSGDSISVYPAQDI
                     TQHNIDTIVEYTEKLAKALHVKGMINIQFIVDGDDVYIIEVNPRSSRTVPYISKVTGI
                     PIVPLATQIICGHTIKELGYNPGLQPAADYIAVKMPVFSFEKIRGADISLGPEMKSTG
                     ECLGIAKTFNEALYKAFEGAGIRLPKYKKMIMTIRKNEQEEAVDIARRFANVGYQIFA
                     TKGTARTLNEHGVKAFEIRKLEQESPNILDLVLGHQIDLIIDIPAQGAERSHDGFVIR
                     RNAIETGVNVLTALDTARALVTSLENRAKELTLVDIATVKNA"
     gene            complement(8687..9760)
                     /locus_tag="ACZPCL_07550"
     CDS             complement(8687..9760)
                     /locus_tag="ACZPCL_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HIR1162354.1"
                     /translation="MKAYLILEDGTVFTGTSIGSQREVISEIVFNTSMTGYLEVLTDP
                     SYAGQAVVMTYPLIGNYGICRQDMESLKPWPDGYIVRELSRIPSNFRCEDTIQNFLEE
                     NDIPGICGIDTRALTKLLREKGTMNGMITTKEYSDLSEPISRMKAYTVAGVVMATTTK
                     EKYVLPGNGKKVALMDFGAKKNIARSLNERGCEVTVYPADTPAEEVLAANPDGIMLSN
                     GPGDPKENVEIIKEIKKMYDSNVPIFAICLGHQLMALANGMDTYKLKYGHRGGNHPVK
                     DLETGRVYISSQNHGYAVDTNTVKPEIAVPAFVNVNDGTNEGLKYTGKNIFTVQYHPE
                     ACPGPQDSGYLFDRFLKMMEVND"
     gene            complement(10040..>11523)
                     /locus_tag="ACZPCL_07555"
     CDS             complement(10040..>11523)
                     /locus_tag="ACZPCL_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162355.1"
                     /translation="TVDPVKGTSSTRYGTYEYEEKPVATISCLAEGTEIYYYITDTSS
                     TVKPDKDTWTKYEGPVSVLFDNEKGGSKYLWAATTTDDGETWLKTSKIQFVYSKKPVE
                     DAVVIGDQAYTSFEKALAAAQDGDTLILNDDVELTDEVTMPEASISIQSGEKGPYLIK
                     STKPLNLNGDLTISDVSWNATTYANGYNFTAGENVTCSSTKDIYAGSASGTAQAKGED
                     NTCYITLSSGKFYVYGTGAAGSTMEGDVEVLAEKEAQLQFAGTKGKSELNGDFTVTVD
                     ATEGNAALSSSYGRTSSGTVSGEFTLTIKGAPKLSGTIYAVQYNSSASWGTLDVTEAE
                     LEELELDTLEKLNKKFQGFETVNSEFDEEDEKTEEEVAAELLSKVKEDEQENSQEKKE
                     TETVIPAEDPETEDKEEAEDKSENELEDKSNEDRSDAAEDVKEEENSDEEDKSAEEED
                     PEETEDADSKEDEEEDAPEVLDADETTEDTSSEEAETISQDEE"
BASE COUNT         2872 a         2782 c         2456 g         3413 t
ORIGIN      
        1 ccattatacc aaacggaaca ggtttatgcc ttttttattg gctgaaatat tgaattttca
       61 aggttcaaca caccacatcg gtgtgggaag ttttagttaa taatacggct gttgtataag
      121 cgccaatgcc ataaaatgct gcatggccta aggaagtctg gcctaaatag ccggatacaa
      181 agtttaatcc taatgccagg atggctgcaa tcagacagtt aactgccaca tgctgaaaat
      241 aagtagggat ccaggcgctt ccaccatgga aattgtcaat ataacagatc agtaagacca
      301 gaactgctgc tccaaggaag gcagtctgtc cgttttttcc gaaaaaacgt tcttttttca
      361 tatgctgcct ccttaaactt tctcttcaaa tttatatccc ataagtcctg aaggcttaat
      421 gagaagtacc aggatcatta ccgcaaatcc aatggcatcc ttataaccag tggaaataaa
      481 gcctgctgtc aggttttcta caattccaag gataataccg cctaccaggg agcctgtgat
      541 gcttccgatt cctccaagga tggcacagga ccagccttta agacctgcta aagcacccat
      601 ggttgggtta atggcgtaga tggtaccaat aaagaaaccg gctgctgctc caagggcacc
      661 gctgatcgca aaggtaactt tgatcagctt ttctgcatta acacccataa ggcctaaggt
      721 ctgcatatcc tgggaagcag ccaacatagc ctttcctgta agagtatgct tgataaacag
      781 ctccagacat accatgatga taatggttcc aatgaggata tataaatgaa gggagctgat
      841 ggttaatggc tgacctgaaa tagtacagat ggtgatcagt ttctggtcta ccagctgtgg
      901 atacgctttg gggttgactg taaatacagc gttggcagta tagcgcagga tcgtggaaat
      961 acctaaagcg gtaataaatc cggttgctgc cagaacccct ttctttctta atggcttata
     1021 gccaaactgg tctactgcca ttgcgatcag tgctccagct gccatggaaa tgagaagcgc
     1081 agggatcagc ggaagtccca gataagttgt tacaaaataa cctgtatatg cacccagcat
     1141 atagatctca ccgtgggtcc agttaatgac tcctacaatt ccaaataccg ttgtccagcc
     1201 tatggcgatc agggagtaga tacctccctg tgccagacca tttataacct gctgcaaaaa
     1261 taccaacata ttgtcaccac ctgtactgtt ttgatctatt ttcctgaaga attactcttc
     1321 atataataca tactttccgt cttttacagt cagtgtcagg taatctctta cccagtcacc
     1381 gttgtcatca aaggtaacgg taccggtaac gccttcatac tcagttgcct ttaatgcttc
     1441 tgcaagagtc tgtcttgtaa gttcaccgcc gttttccttc atggctcttt ctgctgcatc
     1501 tgccagtaca tacatctggt cataagctaa tgctgcaaat ccgtccggaa cttcaccgct
     1561 gtacttttcg ccgtacttgg ttacgaattc ctgtcccttt tcatctgcgc ctgctgctac
     1621 gaatacgccg ctgcaaagaa gaccttcaga agcatctcct gcgatgtcga tgacattctg
     1681 gctgtaaagt ccaggctgtc ccatgatcgg tgcttcaatt cccatctgtc ttgcctgctt
     1741 gatgatcagt gctccgtctg ttgtagttcc gcaaagagcg atacagtcag gatcaaggct
     1801 ctttacagta gtaaggatcg ctgcgaaatc ctggtcagtt gcctgatacg gtacggagtc
     1861 aagaacctgt ccgccgtttg cttcaaactg tgcattgaag ctgtccttta aaccagagcc
     1921 gtaatcgttg ttgcagtaaa gggtaactgc tttggtcttg cccatttttg taaactggtt
     1981 tgcgatctga ggaccaactg ccgcatcctg aaccgccata cggaagaaat aatcactgat
     2041 gcctgttaaa gattttgcac tggagcttac taccaactga ggcatctctg catcccctgt
     2101 aataggaagc atggctgctg tacaggaggt aaggcaggaa ccgccgattg ccagaatgga
     2161 gtcatcatct gcaaacttct gtgctccgtt ggctgctgta gaagcatcac cgccgtcatc
     2221 gtatgtttcc agctctaact tggctcccaa cacaccgcca gctgcgttga tctcttccat
     2281 tgccagctca taaccttttt cctgcttggt accataagca ccgttatcac cggaaagagg
     2341 agctaccaga ccgaatttaa tggtatccac cgctgccttt acatcagatg tgtccgctgc
     2401 agtagtgccg ctttctgaag aagctgctgc ttcagttttg ctttccgctg ctgtagaagc
     2461 cggtgcggaa gaagaggaag aacacccgga aactgtcatt gctgcaactg cagccatcat
     2521 tgcatacaca taaccttttt tcataatcat ttcctccttg cccagagcct tgaaaacaaa
     2581 tgataactac cgctcccggc ggtccgccgg gaagatttca ggtaaaacaa aagaggaaac
     2641 tcagactatt gttctgaatt tcctctttga aatcatttat gccttttcgg ctccgtttgc
     2701 ttatttattt ttgatgtgtc gaatgataca ccagttttta ggtgatgtca agttttttta
     2761 ctaaagtttt ggtaaaaaaa ttcgtttcac cctctgaaat attttgtagc ccctatggat
     2821 caataagtaa aataatcatg gatcagaatg gctaaagaca tgtatacacg catgtaataa
     2881 tccgtaaaat taatgccgca gagagcggaa atattctgca gccgcttcat cagtgtatgg
     2941 cggtggatgt acagatgttc cgctgttttc gtagcattgc agttgtgtgc cagatagata
     3001 cggatggtat gtacatagcc tgtgtgatta taccggtcat ggtccaagag tttctgtaat
     3061 gcctggtttc ccaatacaat agaatactca ttttgatgaa gcgtcccaag aagttccact
     3121 aaaagtcggt tatcaaaggt acagatcccc cgttttgccc cgatccggtc cagcaaacag
     3181 atatgatcgt aggcttcttt tacgcagtca ggcagatccc tgctttcttt taaaggctgg
     3241 gcacctacat aaaagttgat ctcttcattt tccgaaagaa aggtaaggaa aatgctgaaa
     3301 tcatacatgg catctgtaaa tgcttctcca tcggaaaagg gcgccagaaa cataccggtt
     3361 ccatctggca aggtctcacc aatacaggtt tttgacatag aagaaagaaa agagcctact
     3421 tttcccattc cctgccggag actttcctcg tcccggctgt tttttcctgt aaggcagatg
     3481 agaaaaagat atacctgctg ttctttttca aatagtttct tttcactgat atttaatttt
     3541 tttccaagtg caatgcgcag tgtgtccagt ccctggacca gctttctgga aataagattg
     3601 ttttccgtga tctgatccat aagcacatga aggatctctg aaaatgacag ccgttccggc
     3661 agctccagca ccggcagccc ttctttgttt gcgtcttcta taatgtattc cggtatctga
     3721 tcgatataat agcccggctg gatcgccaga ccgctgaccc ctcttttttt cagctggtgg
     3781 atcaggtcct tatgaagaga ctgatcctcc aggccatacc cggtagacac cactaactca
     3841 ccctcattta aagatctggg agtgtccagt atttccatta cattgaccca taaaataggg
     3901 tttgaaacat ttaaaacgcc tgctaccagc ctgactccgc cgaagatctg ccggtccacc
     3961 aggtctttta caagaaatgc catagtgtgc tcctttccgg atacctgcaa gttgaaatgt
     4021 gttcatgtga aaacagcaag atatacaata tgtccattat tatacgcatt tggtgtacag
     4081 tttgcaactt gttttagaac taacttttcg ctagactatt ggcaaatgag gttgatcggc
     4141 cagttctggt cattgttttc agaagatttt tccggtagga ggtaatgtat atgagcaaaa
     4201 aaattttaat cacagaagct gtagaccctg caggcattac tctgcttcag gaccatggat
     4261 accaggtggt catgggcacc ggatatgacg aagagaccct tatgagagag gcacatgacg
     4321 cagacggcgt tcttacaaga aatggtcatt ttacagaacg tgttttaaat tcctgcccga
     4381 agctgcaggt catcggcatg catggtgccg gtgtagactg tattgatgta gaagctgccg
     4441 aacggctggg catccaggta acaaacgcag ctgacgccaa cagcggttct gttgcagaat
     4501 ttacccttgg actgatctta agccttgcaa agaagcttcc acaaagccgt gacggcttac
     4561 gcaaagaagg ctggagcatc cgcccacaga tctctacctt tgatctggaa ggaaagacct
     4621 taggggtact tggaatggga cggatcggca ctgcagttgc cagaaaagct tcctatggct
     4681 ttggaatgaa agtgttagga taccgcagaa accttccagg agatatctgc gcggactatg
     4741 gcattcttac aagtgatctg gacaaagtga tccggaattc cgattttctt tctattcatc
     4801 tgccatatac aaaagatacc ttccatctgg taagccgtga aaagctggaa cttatgaaat
     4861 cagatgcgta tcttttaaac cttggtcgtg gtgaattagt cgaccaggct gctttaaggg
     4921 aactgctgct ttccaaccgt ctggcaggcg ctgcactgga cgtatttgaa ggtgagatcc
     4981 cagatgcaga cgacccgctg ctgcacatgg atcaggtaat tgcaacgcca catattgcag
     5041 gtttaagcaa gcaggctatg gaacggctgt cttatcaggc cgctctgggc attacagagg
     5101 tgttagaagg ccgcaaagtg acttatccgg tcaatcatcc ggagaagtat gcaagtgacg
     5161 tttctgtggc ataattttct caaaccatta accgacaaac cgcctgctgc aggcagccac
     5221 gggcaaaaac gcaggatttg taaacgtgca ttacaacaac agcaaaagca caaaaagcac
     5281 atgaaaacag gcatttaaaa cctgcttcca tgtgcttttc tttcgtttaa agaacctgtc
     5341 accggcagtt tttcttccac tgcacgtctg cacaagtgaa cccgcgcctg gcgcgggtcc
     5401 tctctcactt gtttttatcg tcagcataca aaccccgttg cttcggcggg gcttatattt
     5461 atacaccttt atgcattctt cacagttgca atatccacca gtgtcagctc ttttgcacgg
     5521 ttctcaaggc ttgttacaag agctcttgct gtgtcaaggg ctgtaagtac atttacacct
     5581 gtctcaatgg cattacggcg gatcacaaag ccgtcatggc ttctttctgc gccctgtgcc
     5641 gggatatcga tgatcaggtc gatctgatgt cctaatacca ggtcaaggat attcggggac
     5701 tcctgctcca gtttgcggat ctcaaaggcc tttactccat gctcatttaa ggttcttgca
     5761 gttcccttgg tagcaaagat ctggtagcct acatttgcaa atctgcgggc aatatccact
     5821 gcctcttcct gctcattctt tctgatggtc atgatcatct tcttgtactt cggcagacgg
     5881 atgccagcgc cctcaaatgc cttgtaaaga gcctcgttaa aggtcttagc gataccaagg
     5941 cactcaccgg tagacttcat ttccggtcca aggctgatat ctgcgccgcg gatcttctca
     6001 aaggagaata caggcatctt aacagcgata taatcagctg ccggctgcag acctggattg
     6061 taacccagtt ccttaatggt atggccgcaa atgatctggg ttgccagcgg aacgatcggg
     6121 ataccggtta ccttgctgat atatggtacg gtacgggaag aacgtgggtt tacctcgatg
     6181 atgtatacgt catccccgtc tacgataaac tggatattga tcatgccctt tacatgaaga
     6241 gccttagcca gtttctcagt atactctaca atggtgtcga tattatgctg tgtaatatcc
     6301 tgtgccggat atacggagat actgtctccg gagtggatac cggtacgctc aatatgctgc
     6361 atgataccag ggatcaggat gtctgttccg tcgcagatgg cgtctacctc gatttcctta
     6421 cccataatgt acttatctac caggatcgga tgctcctggg cgatctggtt gatgatgccg
     6481 ataaattcgt caatatcttc atcacagata gcgatctgca tgccctgtcc acccagtaca
     6541 taggaaggac gtactaaaac aggatagcca aggtcatttg cagccttctt tgcctcttca
     6601 gcggtaaata cagtgtgtcc tgcaggtctc gggatatggc actgctccag gatctcatca
     6661 aaacgttccc tatcctctgc tgcatctaca tcttctgctg ctgtaccaag gataggcaca
     6721 cccattttca tcagtgcctc ggtcagctta atagcagtct gtccgccaaa ctgtactaca
     6781 gcgccgtctg gtttttcaat atctacaata ctctctacat cttctggtgt taatggctca
     6841 aaatacagct tatctgcaat atcaaagtct gtacttacag tctccgggtt attgttgatg
     6901 ataatggtct catatccttc tttgctgaag gcccaggtac tgtgtacgga gcagaagtca
     6961 aactcgattc cctgtccaat acggatcggg ccggaaccaa gaaccaggac tttctttctt
     7021 ccttctgtct tttcagcctc attgaagctt ccaaaacagg agtaataata tggtgtctct
     7081 gcagcaaact cagctgcaca ggtatcaacc attttgtatg cagcgcggat gccattgtca
     7141 taacgctgct tcttgatctc ttcctgagga ataccggtaa gacgggaaat tacattatca
     7201 gggaactcaa tgcgttttgc ttctgcaagg agctctgtag taagctcttt ctttccttct
     7261 cctactgcct ttaattcttt ctccatttct accaggatag ccagcttgtc aataaaccag
     7321 atatcgatac gggtgatctc atggatcagc tcatatggga agccgcggcg cagagcctct
     7381 gcaattctcc agatacgcat atcgtctacg atctccagct cttttaccag tcctgtatag
     7441 gaaagtccgg taaagtcata ggacatcagg cagtctacat gctgctccag ggaacggatg
     7501 gccttcatca gggcgccctc aaagttggtg cagatactca tgacctcacc agttgccttc
     7561 atctgggttc ctaaggctct cttggcgctt ataaacttat caaatggaag tcttggcatc
     7621 tttactacgc agtagtcaag cattggctca aaacttgcat aagttttctt tgttactgca
     7681 tttttgatct catccagagt atatcctaaa gcgatctttg cagctacttt tgcgatcgga
     7741 tatccagttg ccttagatgc cagtgcggaa gaacggctta cacgaggatt tacctcaatt
     7801 acgcagtatt caaagctgtc tggatttaaa gcatactgta cgttgcagcc gccggtgatg
     7861 cccagttcgc tgatgatatt taatgcagaa gtacgcagca tctggtactc tttatcccct
     7921 aatgtctggg aaggagccac aacaatactg tcacctgtat gaacgccaac cgggtccagg
     7981 ttttccatgt tgcagacggt gatcacgttt cctgcaccgt cacgcattac ttcgtattcg
     8041 atctctttcc agcctgcgat gcagcgctct actaaaacct gtcctacacg ggataaacgg
     8101 agaccatttt ccaggatctc agcacactgc tccggattct gtgcaatacc gccgccggaa
     8161 ccgcccagtg tataagcagg acgaagaact accgggtaac cgatctgtgc tgctacttcc
     8221 agaccatgtt ttacatcttc tacaatgtca gaaggtgcaa ccggctcacc gatcttttcc
     8281 atggtctcct tgaacatttc acggtcttct gcttttttaa tggtaagagc agtggtaccg
     8341 atgaggcgca cattgtgctt ctctaagaat cctgcgtctt ccagttccat tgcaaggttt
     8401 aatccggcct gtccgccaag ggttggcaga atgctgtccg gcttttcttt taagatcagc
     8461 tgttctacta cttctacggt taatggctca atgtatacac ggtctgcaat gtccttgtcg
     8521 gtcatgatgg tagccgggtt ggagtttaaa aggacaactt cgatcccttc ttcttttaaa
     8581 gaacggcagg cctgtgtacc tgcatagtca aattctgctg cctgtccgat tacgatcggg
     8641 ccggagccta aaaccagtac ttttttaatt tcaggattct ttggcatcag tcatttacct
     8701 ccatcatttt aaggaaacgg tcaaataagt atccggagtc ctgtggtcca gggcatgctt
     8761 ccgggtgata ctgtaccgtg aaaatgttct ttcctgtgta ttttaaacct tcgttggttc
     8821 cgtcatttac attgacaaat gcaggaactg cgatctcagg ctttacagtg ttggtatcta
     8881 ctgcgtaacc atggttctgg gaagaaatgt aaactcttcc ggtctccaga tcttttacag
     8941 gatgatttcc gcctctgtgg ccgtatttta atttgtaggt atccataccg tttgccagtg
     9001 ccattaactg atgtcccagg cagattgcaa agatcggtac attggagtca tacatttttt
     9061 tgatctcttt gatgatctca acattttcct ttggatcacc agggccattg gaaagcatga
     9121 tgccgtccgg atttgctgcc agcacttctt ctgcaggtgt atctgccgga tatacggtaa
     9181 cttcgcagcc tctttcattt aaggaacggg caatgttctt ctttgcgccg aaatccatta
     9241 aagctacttt ctttccattt cctggaagaa cgtatttttc tttggttgta gtagccataa
     9301 caacaccggc tactgtatag gctttcatac ggctgatagg ctcagacagg tctgaatact
     9361 cttttgtcgt gatcataccg ttcattgtac ctttttctct taataacttt gtgagggctc
     9421 ttgtatcgat accacagata ccaggaatat cattttcttc taagaaattc tgaattgtgt
     9481 cttcgcaacg gaagttgctt gggatccttg ataattctct tacaatataa ccatctggcc
     9541 agggctttaa agactccata tcctgacgac agattccata attaccgatc agaggatagg
     9601 tcataacaac ggcctgtcct gcgtatgacg gatcagttaa tacttccaga taaccggtca
     9661 tagatgtgtt aaaaacgatc tcgctgatca cttcacgctg ggaaccaata ctggtaccag
     9721 tgaacacagt gccgtcttcc aatataaggt atgctttcat agggggaata cctgtccttt
     9781 cttatgttgt gtagggggag atttacaagc aagcttgaca tccgattgcg cttccaaatg
     9841 tgtgcgctgc tatagacctc agcccaaatt gatagcatta taatatacct tgcataaatt
     9901 ttcaatggat ttgaagacta aatttttttg ccttatacta atcttttttg gcatcaaaat
     9961 tgctaggcag atttatggca aaagagcggg gccaatcggc cccgccctcc atgtttaaac
    10021 cgttttttca gataattttt tattcttcat cctgagaaat ggtttctgcc tcttctgaag
    10081 aagtatcctc tgtggtttca tctgcgtcca gaacttccgg tgcatcttct tcctcatcct
    10141 cttttgaatc tgcatcttct gtttcttcag gatcctcctc ttctgcagat ttatcctctt
    10201 cgtcagagtt ttcctcttct tttacatctt ctgctgcatc ggatctatct tcattggact
    10261 tatcttcaag ctcattttca gacttatcct cagcctcttc tttgtcttct gtctcaggat
    10321 cttctgccgg gatcacagtc tctgtttcct ttttctcctg gctgttttcc tgctcatctt
    10381 ctttcacttt tgacaaaagt tctgcagcaa cttcttcctc tgtcttttcg tcttcctcat
    10441 caaattcgct gtttacggtc tcaaagccct ggaacttctt gttcagtttc tcaagagtat
    10501 ccagttccag ctcctcaagt tctgcctctg ttacgtccag agtaccccag cttgcagaag
    10561 aattatactg aactgcataa atagttcctg aaagcttcgg agctcctttg atagtcagag
    10621 tgaactctcc tgaaacagtt cctgatgaag tacgtccata ggaagaactt aaagctgcat
    10681 ttccctctgt agcgtcaacc gtaactgtaa aatcaccatt taattcagac ttgccctttg
    10741 ttcctgcaaa ctgaagctgt gcttctttct ctgcaaggac ttctacatca ccttccattg
    10801 tgctgccagc tgcgcctgtt ccatatacat agaacttgcc tgatgataga gtgatatagc
    10861 aggtgttatc ttctcccttt gcctgcgctg ttccagatgc agacccggca taaatgtctt
    10921 ttgtagaaga gcaggttaca ttttctcctg ctgtgaaatt atagccgttt gcataagtgg
    10981 ttgcattcca gctgacatcg gatatggtca ggtcaccatt taagtttaat ggcttggttg
    11041 acttgatcag ataagggcct ttttcacctg actggataga aatagaagct tccggcattg
    11101 tcacttcgtc tgtaagctct acatcatcat ttaaaataag tgtatcaccg tcctgtgctg
    11161 cagccagggc cttttcaaag cttgtataag cctgatcccc gatcacaact gcatcttcca
    11221 ctggtttctt gctgtataca aactggatct tggaagtttt cagccaggtt tcaccgtcat
    11281 ctgtggtcgt tgctgcccag agatatttag agccgccctt ttcgttatca aataaaacag
    11341 acacgggtcc ttcgtatttt gtccaggtat ccttatccgg ttttacagta gaggaagtat
    11401 ctgttatgta ataatagatc tctgtgcctt ctgccaggca gctgatggtt gccaccggct
    11461 tctcctcata ttcataggtt ccatatcttg tactggaagt tccctttacc gggtctactg
    11521 taa
//
LOCUS       DBMDUA010000080        11443 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2845_length_11443_cov_3.433874, whole genome shotgun sequence.
ACCESSION   DBMDUA010000080 DBMDUA010000000
VERSION     DBMDUA010000080.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11443)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11443)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11443
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2845_length_11443_cov_3.433874"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="ACZPCL_07560"
     CDS             complement(<1..1030)
                     /locus_tag="ACZPCL_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR1162356.1"
                     /translation="MQNRPTGRSRKRTRRKKGGCGPALLAGFIFLAAGCGAGFLYGKY
                     LKPGTVMADKRDVFQIKGNQVALILDNELQEAKGIYEDGQVYLPVDWVDDYVNQRFYW
                     DDGEKLLVYALPEEIVYADESTEGEAGPLLKEKDGETYLSLGLVKNYTDIREEAFATS
                     EIKRVFIDTDWDTYETATLRHKTALRIKGGIRSEIINTEEKGTSVQILEQMDKWSKVR
                     TEDGYMGYVPNSRLSKVEEETPVSEFNAPVYTNISMDGKVRLGFHQVTTKDANATFDK
                     VADTAQGMNVIVPTWFNITDNEGNYTSLASKDYVDKAHALGIQVWAMFDNISTEESVK
                     NVDSGKLFS"
     gene            complement(1292..1552)
                     /locus_tag="ACZPCL_07565"
     CDS             complement(1292..1552)
                     /locus_tag="ACZPCL_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162357.1"
                     /translation="MKSKKRNEDARHRKDYLDGLKRFKKRGIPIIIDGKECREADWTR
                     IFDVGEDGAFYMGDYVCSDKGGLKEIRFDKVYLSEDQKKKRK"
     gene            complement(1634..2221)
                     /locus_tag="ACZPCL_07570"
     CDS             complement(1634..2221)
                     /locus_tag="ACZPCL_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162358.1"
                     /translation="MVSTVKVTGIIGAGRGTGVTHFSIRAANYFCSWKQRKTAVIQWN
                     SHKDTERMRNFLEASGKEGGRFKKEPFRVLEVDFYCQGGPEVLAACMEKKYQEILIDF
                     GEVRDEVFPEWLRCSEKILIADLSEWKLESFLGLFTEKEKTGKDWICLTAFGSEIVRK
                     EIERQFRLSVKRIPLSVDAFSVDIQTMEWFADILV"
     gene            complement(2206..3621)
                     /locus_tag="ACZPCL_07575"
     CDS             complement(2206..3621)
                     /locus_tag="ACZPCL_07575"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR1162359.1"
                     /translation="MLFGKYRLIQTVGAGRTGTVWKAVHLGLNEYRAIKCVPKTHKDY
                     EEFRREALVLKELKYPGIPLVYDLEEDSHYFYLIEEYLEGNSLYDLIQDQGPFQEAEA
                     VRYGRQICSLVEYLHHSCDKPILHLDLQPKNLMIWKGTVQLIDFDHAGDSDSASLRKE
                     RYGTAGCAAPELYTTDQLLDQRTDIYAIGAILRFMLYGTLKAGAETDCGITVSGPLQN
                     IIKKCMEPDRTKRYPSAKELELALERSVRGGRLISADNNAVSSLNIVIAGSTPGAGAT
                     HLAFGLCVYLTKMGIKVLYEERNQTGAVRRMAESTGGARIDGRGIYHIQGCLMKPWYG
                     PAVKLDTNTEFEVVIKDFGTNWEEAGQTLKEKDHFLTAVISENLWEYGKAKMMLEKMT
                     GSKEENMGPMALVWRHARGKKNKDMTLGHKPFLFLEPEYISPFEPGAEGVSFLRSLWD
                     CIQKAEKKQSMKKRRFSWFRQ"
     gene            3995..4744
                     /locus_tag="ACZPCL_07580"
     CDS             3995..4744
                     /locus_tag="ACZPCL_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HIR1162360.1"
                     /translation="MKIERISDNQIRCTLTSLDLSSRGINLAELAYGSEKARNLFREM
                     IQQAAQEVGFEAEDIPLMVEAIPLSSESIMLIVTKVEDPEELDTRFSKFSPFAEAESE
                     NLLSQLADEFLEGAGDLAKLLGQNGTEELKANVNTAAENTGTENAKAPQIQLRTFRFD
                     SLDQISEAAKAAEDSCDDIHNALYKKPSTRQYYLVLNNKDCNELDFSRVCNVLAEYAA
                     KLPNEYAGEAYYKEHYELMIGEKALQKLKNL"
     gene            complement(4779..5456)
                     /locus_tag="ACZPCL_07585"
     CDS             complement(4779..5456)
                     /locus_tag="ACZPCL_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1162361.1"
                     /translation="MNAQGKRSGETAREYAYRMIREQVISLELKPGEAFNDMEFAAKI
                     GVSRTPVREAVIQLSEESRIIEIFPQRGMRIAPIDVELVEEARSLRVLLEKQIAGMCC
                     EMATEEDIKWLQENVGLQEFYMENSRPEMVMQLDDEMHHKLFMICHRELTYGMCQKLA
                     IHYDRIRSLSVNTVKDNTIIEDHRKIIQFIAEHKKENAMAQMEKHLSRWQLNEGKFRQ
                     EFPDYFK"
     gene            complement(5483..6565)
                     /gene="uxuA"
                     /locus_tag="ACZPCL_07590"
     CDS             complement(5483..6565)
                     /gene="uxuA"
                     /locus_tag="ACZPCL_07590"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HIR1162362.1"
                     /translation="MDMTLRWFGEGIDSVSLEQIRQIPGVKGVITTLYDIPAGEEWPM
                     ERILQMKEQVEAKGLKVMGIESVNIHDAIKVGTPDREKYIANYITTLERLGQADIHVV
                     CYNFMPVFDWTRSDLAKVRPDGSTVLAYDQKEIDKINPENMFESMGEKSNGFELPGWE
                     PERMARIKELFEMYKDVDEEKLFNNLVYFLKAIQPVCEKYDIRMAIHPDDPAWPVFGL
                     SRIITDKEHLLKLMKAVDAPFNGVTLCTGSLGSNPENDIPDIIRSLKGRIHFAHVRNL
                     QYNGYRDFQEAAHLSADGSMDMYEIMKALYDIGFDGIIRPDHGRAIWGEVSMPGYGLY
                     DRALGACYLNGLWEAIVKQNGNAHQE"
     gene            complement(6802..7002)
                     /locus_tag="ACZPCL_07595"
     CDS             complement(6802..7002)
                     /locus_tag="ACZPCL_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HIR1162363.1"
                     /translation="MKIEKTMLIGELIHMDELIPQMLMGAGMHCLGCPASQAESLEEA
                     CMVHGIDCDDLVSKMNEILADR"
     gene            7317..7484
                     /locus_tag="ACZPCL_07600"
     CDS             7317..7484
                     /locus_tag="ACZPCL_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR1162364.1"
                     /translation="MAYVITDGCVSCGTCAGECPVGAISEGDGKYVIDADSCIECGTC
                     AAACPTGAIEG"
     gene            7574..8296
                     /locus_tag="ACZPCL_07605"
     CDS             7574..8296
                     /locus_tag="ACZPCL_07605"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR1162365.1"
                     /translation="MTQKKPIIGITPSHNTENNDTSLRPTYPKAIAAAGGLPILLPLE
                     CSDEDIKQFMDVCDGFLFTGGPDINPFLFGEDTHLKCGNISAARDHLEFRLLSAAMDA
                     GKPIFGICRGVQVLNVGLGGSLYQDIPSQTEREFPIAHQQPFYYNIPCHRVNITENTL
                     LHSICHGETQIAVNSSHHQCVHIPAPGLVVSAVAPDGIIEAVEKPDYPNFFLGVQWHP
                     EYLWDRDEAAMGLFQAFVDACK"
     gene            complement(8332..9234)
                     /locus_tag="ACZPCL_07610"
     CDS             complement(8332..9234)
                     /locus_tag="ACZPCL_07610"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HIR1162366.1"
                     /translation="MKILNFGSLNIDYVYSVDHFVQKGETISSSQMNIFSGGKGLNQS
                     TALGRAGAQVFHAGRIGKGGEFLVEQLKEAGVNTDLVVLDSQCSTGHAIIQNDAEGDN
                     CILLYGGANQRITKEQIDATLDQFDAGDYLVLQNETSNLPYLMEKAAEKQMKIVLNPS
                     PMDEKIKALPLDKVTLFFVNEIEAAQILGQKELESTQEAAAAFRSMFPQAAVVLTLGS
                     QGSVYIDKDQVIPQKCYKVKAVDTTAAGDTFTGFFVSAISKGKSPKEALDLASRASAI
                     AVSRPGAAPSVPTLDEVENWEFAE"
     gene            complement(9478..11085)
                     /gene="pckA"
                     /locus_tag="ACZPCL_07615"
     CDS             complement(9478..11085)
                     /gene="pckA"
                     /locus_tag="ACZPCL_07615"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR1162367.1"
                     /translation="MAAKIDLTKYGITGTTEIVYNPSYEMLFEEETKPELEGYEKGQV
                     SELGAVNVMTGIYTGRSPKDKFIVVDENSKDTVWWTSDEYKNDNHPASQEAWKAVKEI
                     AQKELSNKKLYVVDAFCGANKDTRMAIRFIVEVAWQAHFVTNMFIKPTAEELENFEPD
                     FVVYNASKAKVENYKELGLNSETAVVFNITSREQVIINTWYGGEMKKGMFSMMNYYLP
                     LKGIASMHCSANTDMNGENTAIFFGLSGTGKTTLSTDPKRLLIGDDEHGWDDNGVFNF
                     EGGCYAKVINLDKDSEPDIYNAIKRNALLENVTLDKEGKIDFADKSVTENTRVSYPID
                     HIEKIVRPISAGPAAKNVIFLSADAFGVLPPVSILTPEQTQYYFLSGFTAKLAGTERG
                     ITEPTPTFSACFGQAFLELHPTKYAAELVKKMEKSGAKAYLVNTGWNGTGKRISIKDT
                     RGIIDAILSGDILKAPTKKIPHFDFEVPTELPGVDPAILDPRDTYADAAEWEKKAEDL
                     ASRFIKNFAKYESNEAGKALVAAGPQK"
BASE COUNT         2897 a         2771 c         2234 g         3541 t
ORIGIN      
        1 aagaaaacaa tttgccggag tctacatttt tcacgctttc ttctgtactg atattgtcaa
       61 acatggccca tacctgaatt cctaaagcat gtgccttatc tacataatcc ttgcttgcaa
      121 gggacgtata attgccctcg ttgtctgtta tattaaacca ggtaggcacg attacattca
      181 taccctgggc cgtatctgca accttgtcaa aggtagcatt tgcatccttt gttgtcacct
      241 gatgaaagcc cagacgtact tttccatcca tggaaatgtt tgtatacaca ggcgcattaa
      301 attcgctgac cggtgtttct tcctccactt tgcttaaacg gctgtttggc acatagccca
      361 tataaccatc ttctgtgcgc actttagacc atttatccat ctgctctaag atctgcacag
      421 aggttccctt ttcttctgta ttgatgatct cactgcggat accacctttt atccgaagag
      481 ctgttttatg acggagtgtg gctgtctcat atgtatccca gtccgtatca atgaaaactc
      541 ttttgatctc actggtagca aaagcctctt cccggatatc cgtataattt ttcacaagcc
      601 ctaaagaaag ataggtttcc ccgtcctttt cttttaaaag aggccctgct tccccttctg
      661 tggactcatc cgcatagacg atctcctccg gaagggcata aactaaaagt ttctccccgt
      721 catcccagta aaaacgctgg tttacatagt catccaccca gtctaccggc agatatacct
      781 gtccatcctc gtaaatacct tttgcttcct gaagctcgtt atctaagatc agagccacct
      841 gattcccttt gatctggaaa acatcccgct tatctgccat caccgttccc ggttttaaat
      901 attttccata taaaaatccg gctccacagc ctgctgcaag aaatataaaa cctgcaagca
      961 acgccggtcc gcagccgcct ttttttcttc tggtacgttt tctgcttcgt cctgttggtc
     1021 tgttctgcat atcttttccc ttttcattct gtatgtactc tcaaaaccct tactgccgta
     1081 cttttatctg ttctgctata tttccaacta ttatgcagtt tatcacttat ttttaaacgt
     1141 aacaactata tatcagaaaa ctttaaacgt tttgaaacca tttcccggta caaaaaaata
     1201 cccggtggac agccatattt gcttgcaggc cgagatggtt attttctcga tttactgtcc
     1261 accggatatt ttatcttttt atctttttct tttattttct ctttttcttt tgatcttctg
     1321 aaagatatac cttatcaaaa cggatctctt ttaaaccgcc tttatcggaa cagacatagt
     1381 ctcccatata aaaggctccg tcttctccca catcaaagat cctggtccag tctgcttccc
     1441 tgcattcttt tccgtcaatg ataataggga tcccccgttt cttaaaacgt tttaaaccat
     1501 ccaggtaatc ttttcgatgt cttgcatctt catttctttt cttgcttttc atactgtaac
     1561 tttcccccac cagaccgcat cctgtttcgg aggccctgtg acatatatct tccaggatac
     1621 ggccagtttt cttttatacc aatatatccg caaaccattc catggtctga atgtccactg
     1681 aaaatgcatc tactgaaaga gggatccgtt tcacagaaag gcgaaactgt ctttctattt
     1741 cttttctcac gatctcgctg ccaaaagcag tcaggcagat ccagtctttt cccgtttttt
     1801 ccttttctgt aaacagcccc aaaaatgact ccagtttcca ttctgacagg tctgcgatca
     1861 ggatcttttc agagcaccga agccactccg gaaagacctc atctctcact tctccaaagt
     1921 caatgaggat ctcctgatat tttttttcca tacacgcagc cagtacctcc ggtccgccct
     1981 gacaataaaa atccacctcc aggacccgga aaggttcttt cttaaagcgg cctccttctt
     2041 ttccggaagc ctccagaaaa tttctcatcc gttctgtatc cttatgggaa ttccactgga
     2101 tcacagctgt tttcctctgt ttccagctgc aaaaataatt agccgcccgg atactgaaat
     2161 gagttacccc ggttcccctt ccagctccta tgatcccggt tacttttact gtcgaaacca
     2221 cgaaaatctc ctttttttca tgctctgctt tttttccgct ttctgtatac aatcccagag
     2281 agatcttaaa aagctgactc cctctgctcc aggttcaaaa gggcttatgt attccggctc
     2341 cagaaaaaga aagggcttat gccccaaagt catatcttta ttcttctttc cccgggcatg
     2401 ccgccagaca agagccatcg ggcccatatt ttcttctttc gatccggtca tcttctccaa
     2461 catcattttt gccttcccgt actcccacag gttttcactg atcactgccg taagaaaatg
     2521 atccttctct tttaatgtct gtcctgcctc ttcccagttt gtgccaaaat cttttataac
     2581 cacttcaaac tctgtatttg tatccagctt cacagccgga ccgtaccagg gtttcataag
     2641 acatccctga atatgataga taccacggcc atcaatccgc gccccgcctg tactttctgc
     2701 catgcgcctt actgctcctg tttgatttct ttcttcatac aggactttta tccccatttt
     2761 ggtaagatag acgcaaaggc caaaagccag atgggtagct ccggctcccg gtgtactgcc
     2821 tgcgatcaca atattcagag atgataccgc attattatct gcgctgatca ggcgcccgcc
     2881 ccgtacagaa cgctccagtg ccagttccag ttcttttgct gatggatagc gctttgttct
     2941 gtctggttcc atacattttt taatgatgtt ctgaagcggc cctgatactg tgatcccaca
     3001 gtctgtttct gcccctgctt tcagggttcc gtatagcata aaccggagga tggcaccaat
     3061 ggcataaata tccgtccttt ggtctaaaag ctggtcagtt gtatatagtt caggtgctgc
     3121 gcagcctgca gtaccatacc gttcttttct taaactggcg ctgtcgctgt ctccggcatg
     3181 gtcaaaatcg atcagctgta ccgtgccttt ccagatcatc agatttttcg gttgtagatc
     3241 taaatgtaat atgggtttat cacaagaatg atgtaaatat tcaactaatg aacagatctg
     3301 ccgcccatag cgaacggctt ctgcttcctg aaatggtccc tgatcttgga ttaggtcata
     3361 cagagaattg ccttcaagat attcctcaat aagataaaag tagtggctgt cctcttccag
     3421 atcataaacc agtgggatcc ccggatactt aagttcttta agcaccaacg cttctcttct
     3481 gaattcttcg taatctttat gcgttttcgg tacgcactta atggcccggt actcgtttaa
     3541 tcccaggtga actgctttcc atacagttcc cgtgcgtcct gctcccactg tttgaatcag
     3601 ccggtatttt ccaaacagga tctccctttc gtttcacctc tctttcgtta caatatcatc
     3661 tcctttggct atcttatctg ataagctgtt acttgtctat ggtctatata atgactacac
     3721 atatagctgg gacatggtgt atacatcttt tcattcacat tctttcttag aagtcaaaga
     3781 aaaacgccat ttcctgtgtt ttttctttta tcttctgctc atatagctca tttctgttga
     3841 taaatgcaca ctaaaaattt taaatatata ttattgatat ttaattgata tttccaccca
     3901 ttatgtagta tattctaatt gtagaagttt atcttcataa cacgtggcac taaaacacac
     3961 gacaaaacac aaagagaaca ggaagtgatc ctatatgaaa atagaacgaa ttagtgacaa
     4021 tcagatacgc tgcaccctga caagcttaga tctaagctca cgggggatca atctggcgga
     4081 actggcttat ggctctgaaa aggccagaaa cctgttccgt gaaatgatcc agcaggcagc
     4141 acaggaagtt gggtttgaag cggaagatat tccgttaatg gtagaagcca ttcctctgtc
     4201 ttccgaaagc attatgctta ttgtgacaaa agtagaagat ccggaagagt tagatacccg
     4261 cttctccaag ttttctcctt ttgcagaagc agagtcagaa aatctccttt cccagctggc
     4321 agatgaattt ctggaaggcg ccggtgacct ggctaaactt ctgggacaaa acggaacaga
     4381 ggaacttaag gcaaatgtaa atacagctgc cgaaaacaca ggaacagaga atgcaaaagc
     4441 tccacagatc caactgcgca ctttccgttt tgacagtctg gatcagatca gtgaagctgc
     4501 aaaagctgcc gaagacagct gtgatgacat tcacaacgca ctttataaaa aaccatccac
     4561 ccgtcagtat tatctggtat taaataataa ggactgcaat gaactggatt tctccagagt
     4621 ctgcaacgtg ctggctgaat atgcggccaa actgccaaat gaatatgcgg gagaagccta
     4681 ttacaaagaa cattatgaat taatgatcgg agaaaaggct ttgcagaaac taaaaaatct
     4741 gtaaaaatta acgccccgga tatccggggc gcgtttgttt atttgaaata atccgggaat
     4801 tcctgacgga attttccttc atttaactgc caccggctca aatgcttttc catctgggcc
     4861 atggcatttt cttttttatg ctcggcaata aactggataa ttttcctgtg atcttcgata
     4921 atggtattat ctttcactgt atttacagaa aggctgcgga tacggtcata gtggatagcc
     4981 agtttctggc acataccata agtcagttct ctgtggcaga tcataaacag cttatgatgc
     5041 atctcatcat ctaactgcat taccatttcc ggccgggaat tttccatata aaattcctgc
     5101 agtcctacat tttcctgcag ccattttata tcttcttcgg ttgccatttc acagcacatt
     5161 cccgcgatct gcttttctaa aagcacacgc agggaacggg cttcttctac cagttccaca
     5221 tcgattggcg caatgcgcat accgcgttgg gggaatattt ctataatacg tgactcttcg
     5281 cttaactgga tcaccgcctc ccgcacagga gtccggctaa cgcctatttt tgccgcaaat
     5341 tccatatcat taaaggcttc acctggcttt aattccagag aaataacctg ttcccgtatc
     5401 atacggtatg cgtattccct ggctgtttct cctgatcttt ttccctgtgc gttcatggtt
     5461 tcatttctcc tgttttctgc ttctactcct gatgtgcgtt tccattctgc tttacgattg
     5521 cttcccacag tccgttcagg tagcatgcac ccaacgcacg gtcatacagg ccatatcctg
     5581 gcatggaaac ctcaccccag atcgctcttc cgtggtctgg gcggataatg ccgtcaaagc
     5641 cgatatcata cagcgctttc atgatctcat acatatccat gcttccgtca gctgataagt
     5701 gagctgcttc ctggaaatct ctgtaaccat tatactgcaa gttgcgcaca tgtgcaaaat
     5761 gaatacgtcc ctttaaagaa cggatgatgt ccgggatatc attttccgga ttgcttccta
     5821 aagatccggt gcagagagtt acgccgttaa atggtgcatc tactgccttc atcagtttta
     5881 agaggtgctc cttatcggta atgatacggg aaagaccgaa aacaggccat gcaggatcat
     5941 ctggatggat cgccatacgg atatcgtact tttcacatac aggctggatt gcttttaaga
     6001 aatataccag gttgttaaat aatttttctt catctacatc cttgtacatt tcaaacagct
     6061 ctttaattct tgccatacgc tccggctccc agccaggaag ttcaaaacca ttggacttct
     6121 cacccatgga ctcgaacatg ttttccggat tgatcttgtc gatctctttc tggtcatatg
     6181 ctaaaacagt agaaccatcc gggcgtacct ttgccagatc agaacgggtc cagtcaaata
     6241 caggcatgaa attgtagcag actacatgga tatctgcctg tcctaagcgc tccagggtag
     6301 tgatatagtt tgcaatgtat ttttcacggt ccggtgttcc taccttaata gcgtcatgga
     6361 tatttacgct ttcaattccc attaccttaa ggccttttgc ttctacctgc tccttcatct
     6421 gaagaatgcg ctccataggc cactcttcgc ctgcagggat atcatataaa gtagtgataa
     6481 cgccctttac tcctgggatc tggcggatct gctctaaaga aacagagtca attccttcac
     6541 caaaccaacg caatgtcata tccataatgt ttacctcctg agtgtttaaa aattttaaat
     6601 tatttttgtt ttcccaaatc agtgttgtgt taatatacta atatattact caatatacta
     6661 agtatcggcc caattgtcaa gagtgctttt gtaaattaaa cgcaaaaacc gcctgctgca
     6721 aagtgctttt caacttgcac tttacagcag acggtttttt acatcgggtc tttacccgta
     6781 catgttctct tgtggataaa cttatctgtc agccaggatc tcattcatct tggaaaccag
     6841 atcatcgcag tcgattccat gaaccatgca tgcctcttcc agagattcag cctgagaagc
     6901 tgggcatcca aggcagtgca ttcctgcacc catcagcatc tgagggatca gctcatccat
     6961 gtggatcagt tcaccgatca gcattgtctt ttcgatcttc atgtgtattt cctccttata
     7021 ttctatatac tatacgtaaa tatgctatac gcttttatca gcataataca ttttggattt
     7081 tttggcaagc ctatttcttt tgtaggttta cctttaaact cgcaaaccct tgtatttact
     7141 ggcgttttag atagttttga ctaaccattt tgctcctaaa tttgtacaat ttatacagct
     7201 ttagggggta ttttcctctt gaataattgt ccagaatcgt ttacaataaa gaaacaataa
     7261 acgagattgt ttttgaagtt tcaaaaacat ctaaatatat aaggaggtat tttatcatgg
     7321 catatgtaat tactgatggt tgtgtaagct gcggaacctg tgctggcgaa tgtccagtag
     7381 gtgcaatctc tgagggagat ggcaagtacg ttatcgacgc tgattcctgc atcgagtgcg
     7441 gaacctgcgc tgctgcctgt ccaaccggcg ctatcgaagg ctaattataa gctacataag
     7501 aaaaccacag agcatttgct ttgtggtttt tttttctatc atataattaa tgtcaaaaag
     7561 gacgtaatac accatgactc aaaaaaagcc catcatcggc atcacaccat cacacaacac
     7621 tgaaaataat gataccagcc tccgtcctac ctatccaaaa gccatcgccg cagcaggcgg
     7681 actgccgatc ctgcttccgt tggaatgcag cgatgaggat ataaaacagt ttatggacgt
     7741 ttgtgacggt tttcttttta caggaggacc ggacattaat ccatttcttt ttggggaaga
     7801 tacccattta aaatgtggaa atatatctgc tgccagagac cacctggaat ttcgccttct
     7861 ttcggctgct atggacgccg gaaagccgat ctttggcatc tgccgaggtg ttcaggtatt
     7921 aaatgtaggc cttggtggaa gtctgtatca ggatatccca tcccagacag aacgggaatt
     7981 tcctatcgca caccagcagc cgttttacta taatatccca tgtcaccggg tgaatattac
     8041 agagaatact ctccttcact ccatctgcca cggtgagacc cagattgctg taaacagcag
     8101 ccatcaccag tgtgtgcata ttccggcacc gggactggta gtttctgcgg ttgctccgga
     8161 cggtattatc gaggctgttg aaaagcctga ttacccgaat ttcttcctgg gagtgcagtg
     8221 gcatccggaa tatctctggg acagagatga ggctgctatg ggactgtttc aggcatttgt
     8281 ggatgcgtgt aaataacatt tggttttgca cattgagaaa ctgagatctg cttactccgc
     8341 aaattcccag ttctcaactt catctaaagt cggtacagat ggtgctgccc ctggtcttga
     8401 cactgcaatt gcagacgctc ttgatgccaa gtccagtgcc tcttttgggc ttttgccctt
     8461 gctgatggcg gatacgaaga aaccagtaaa agtatcgcct gcagctgtgg tatctactgc
     8521 ttttacttta tagcactttt gtgggatcac ctggtctttg tcaatataaa cagaaccctg
     8581 agagcctaac gtaaggacga ctgctgcctg cgggaacatg gagcggaaag ctgccgctgc
     8641 ttcctgtgta ctttccagtt ccttctgtcc taagatctgg gctgcttcaa tctcattcac
     8701 aaagaacagc gttaccttgt ccaatgggag cgcttttatt ttttcatcca ttggggacgg
     8761 atttaacacg atcttcatct gtttttcagc tgctttttcc atcagataag gcagattgct
     8821 ggtctcgttc tgtaagacta aataatctcc tgcatcaaac tggtctaatg tagcgtcgat
     8881 ctgttctttt gtgatccgct gattagcacc gccatagaga aggatacagt tatctccttc
     8941 tgcatcattt tggataatgg catgtcctgt ggaacactgg ctatctaaaa ctaccagatc
     9001 tgtgttcaca ccagcttctt ttaactgctc tacaagaaat tctccccctt ttccgatcct
     9061 tcctgcatgg aaaacctgcg ccccggcgcg tcctaatgca gtagactggt ttaagccttt
     9121 tccgccgctg aaaatgttca tctggctgga agagatagtt tcgccttttt gtacgaaatg
     9181 gtctacgctg tatacataat cgatattcag tgaaccgaag tttaatattt tcatgtaaat
     9241 aacccccatt tgcatttttt aacattatag catacttaca atccctgcac tagcgtgctt
     9301 gtgccgcctg cgtaagcagg cggaattttc tcatttatag cgcaaaaaaa cgcagcccca
     9361 taagctacag gactgcgttt ttcttatacg ttacgccggc tctcatcttg cgtgacctgt
     9421 cggtcccaca agatgcatcg gatggtcatc cgatgtaaga tccagtcttt tttcgtttta
     9481 tttctgtggg ccagctgcta ccagtgcttt accagcttcg ttgctctcgt acttagcgaa
     9541 gttcttgatg aatctgcttg ccagatcctc tgccttcttc tcccactcag ctgcatctgc
     9601 gtaggtgtcg cgtgggtcaa ggattgcagg atctacacct ggaagttcgg taggtacttc
     9661 gaaatcgaag tgagggatct tcttggtagg tgcctttaag atatcgccgc ttaagatagc
     9721 gtcgatgata ccacgggtat ccttaatgga gatacgcttg ccggtaccgt tccatccggt
     9781 atttactaag tatgccttag cgccgctctt ttccatcttc ttaaccaact cagctgcata
     9841 ctttgtagga tgcagttcca ggaatgcctg accgaagcaa gcagagaagg taggtgtagg
     9901 ctcagtgatt ccacgctctg taccagctaa tttagctgta aatccagaca ggaagtaata
     9961 ctgggtctgc tctggtgtca ggatagatac tggaggcagt acgccgaaag catctgcgga
    10021 caggaagata acgttctttg ctgcaggacc tgcagagatt gggcgaacga tcttctcgat
    10081 gtggtcgatt gggtaggaaa cacgggtgtt ctcggttacg ctcttatcag cgaaatcgat
    10141 ctttccttct ttatccagag taacgttctc aagcagagcg tttctcttga ttgcattgta
    10201 gatatctggc tcagaatcct tatccaggtt gataacctta gcgtagcatc cgccttcaaa
    10261 gttgaatacg ccattgtcat cccagccgtg ctcatcatca ccgatcagca gacgcttagg
    10321 atctgtagac agagtggtct tacctgtacc ggacagaccg aagaagattg cggtgttctc
    10381 accgttcata tctgtatttg cggagcagtg catggaagcg atgcccttta atggcagata
    10441 gtagttcatc atggagaaca tacccttctt catctcgccg ccgtaccatg tattgatgat
    10501 aacctgctca cggcttgtga tgttgaatac aactgcagtc tcagagttca gacctaattc
    10561 cttgtagttc tctactttag ccttggatgc gttgtaaaca acgaaatctg gctcgaagtt
    10621 ttccagctct tcagcagttg gcttgatgaa catgttggta acgaaatgtg cctgccaagc
    10681 tacttcaacg ataaaacgga ttgccatacg agtgtccttg tttgcgccgc agaatgcatc
    10741 tacaacgtaa agtttcttgt tggacagctc tttctgagcg atctctttaa cagccttcca
    10801 agcctcctgg gatgctggat ggttgtcgtt cttgtattca tcagaggtcc accatacagt
    10861 gtccttggag ttctcgtcta caacgatgaa tttgtcttta ggagaacgtc ctgtatagat
    10921 gccagtcata acgttaactg ctcccagttc gctgacctgt cctttttcat aaccttccag
    10981 ttctggtttg gtttcttcct caaataacat ttcataggaa ggattgtaca caatctctgt
    11041 ggttccggta atgccatact ttgttaaatc gatttttgct gccatcttac taacctcttt
    11101 cctttcgtaa ttgatcataa gggatagatg cgattgtatt ttttcgcatg cactacagat
    11161 tatcccacta atttgttaaa atgtcaacaa aggatagtag taatatttta acgtattttt
    11221 atcagttttt actgcattta ttcggcgaat tgtaaaaatt gtaaaaaaag gatctccgtg
    11281 ttaaaatagt tgggaataga gatactggga acgaatacga aaaaagtaat tttgaaactg
    11341 aaacatcagg aggatcttac atgacaactc tgccggaaaa aggcgaagag atttttggat
    11401 ataaagtgat ggcccagacc actgcttaaa atcaaaattc cgc
//
LOCUS       DBMDUA010000081        11307 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2889_length_11307_cov_3.897644, whole genome shotgun sequence.
ACCESSION   DBMDUA010000081 DBMDUA010000000
VERSION     DBMDUA010000081.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11307)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11307)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11307
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2889_length_11307_cov_3.897644"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            131..1309
                     /locus_tag="ACZPCL_07620"
     CDS             131..1309
                     /locus_tag="ACZPCL_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR1162368.1"
                     /translation="MSLITVVQKMFTGLNSFTLLAVPLFVLAANIMNRAKISEKLIQF
                     CNGIVGRFPGGLAYANVLVSMLFAGISGSSQADTAGIGSILIPAMEEEGYNQETSVGV
                     TAASSTIGIVIPPSIPMVVYSSVAGASIGALFLGGVVPGILIGLGQMFVIFMGNKKYH
                     YPKQDPMSFKDFLKLAIKCLPPVLTPVIIIGGVIGGLCTATESAAIACIYAIILGVFV
                     FKNLSLKDVKPLLYDTLRTSATSLFALATANALGQLLSYYNVSTSVQQMFATYFPYKW
                     QFLLAVIGFYLFLGTFMDAIPAMILFVPVLMPVATAFGITPVQLGLIIVITLAVGQVT
                     PPYGLCLLIAGRISGMSVQKSFKAVIPYIMVSVVVVVLIAFLPDIAFALPKLIKPEWF
                     "
     gene            1337..2731
                     /locus_tag="ACZPCL_07625"
     CDS             1337..2731
                     /locus_tag="ACZPCL_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019496.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HIR1162369.1"
                     /translation="MEKKKVAIVTLGDSRREFYKKREAIVASEIEKVKKAFGDKYDLY
                     ISPIVFDTEDGQNISDEIKKQGIEAVIIHVPIWATPSLAFRIAYGTSYPVLLLGNLQR
                     NTSSLVTLLAVGGMLDQTGKKCIRVSGDYEDPQIQEKVDNFVKGVYVAEGIRRSSYGM
                     IGGRSIGIGTTVADPSQWQSTFGVEFDHCDQYEIVYRAKALDEERVQKHLKWVKENVP
                     RIEYGDKFTEKTLELQVRSYLALKDMAAEKKYNFMGIKCQQDMSDHFVLQCLGVALLN
                     NPYDADGEKTPIPTSCECDCDGAMTMRILNLCAEGKPSCLLDIKFFDGNDKEFVLANC
                     GSVAPYFADPDNKEEAMKKIALMPHIFGEAGGASIQFISKPGDVTVARLFRSNGKYVL
                     GCFEGTTKMYPLEKLKETTYCYPYAFVEAEADYEKFYEKINSNHLHMVYGKYAKVLKM
                     FCEIVGIEYICFNK"
     gene            2965..3174
                     /locus_tag="ACZPCL_07630"
     CDS             2965..3174
                     /locus_tag="ACZPCL_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162370.1"
                     /translation="MRLLYELSWLWEMMGIAMAAAVVCVACAALICKAAKKKLSRKVI
                     AVVAAAAFAGAVLAVILIARTPMPL"
     gene            3285..3926
                     /locus_tag="ACZPCL_07635"
     CDS             3285..3926
                     /locus_tag="ACZPCL_07635"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1162371.1"
                     /translation="MNKVITVSREFGSGGRELGVKLAEALGIPFYDKELISMAADDIN
                     IAEEAFRKYDEHVIEDDPLDHKIHHSFSELYKVPMSDQIFVAQSNVIRRLASHGPCVI
                     VGRCADMILEDSVNIFIYSKMKQRIKRLMELEPEEGINEKDMEMKIRAVDRKRRDYYQ
                     YYTGNTWGRAQNYHLCVDSSLVGVDGCLRAALAYLEDVCKDEEKPEDGEKETV"
     gene            4037..5179
                     /locus_tag="ACZPCL_07640"
     CDS             4037..5179
                     /locus_tag="ACZPCL_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR1162372.1"
                     /translation="MEKPGKKLKKFFIITGITGMVYAGLKYLLPLVVPLLLGYTLALL
                     LRPSARFLQHRITVRIFGKKWKPSLGLIGSVQLLILAVLGSFLLTLGIRRLWEETSTL
                     MNQLPVWLDGLYFWIEEKCSGLEKRFGLDPGWGIHVAGSMIEAGTKYVKEKSVPSLMA
                     GSYYTAGVIGRIGFISLFSFCSAILSLQEMEELKERRDRSVFSREFRLIGDRLVSTGK
                     VWFKSQGIIFLLTSFLCTIGMFLVGNPCPVLVGMGIGIMDAFPVLGTGTVLVPWAVIE
                     IAMGSWKTALILTGLYLICTFMRQFLEVHLMSGQMGLSPFETLGAVYVGLKLFGIAGL
                     FLGPLGLLLIEDMTELLITQPMNDEQRHNETNKSAMIDGGNRFMSH"
     misc_binding    5250..5487
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5726..7165
                     /gene="proS"
                     /locus_tag="ACZPCL_07645"
     CDS             5726..7165
                     /gene="proS"
                     /locus_tag="ACZPCL_07645"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR1162373.1"
                     /translation="MADNKKMVEAITSMDTDFAQWYTDVVKKAELCDYASVKGCMVIK
                     PAGYAIWENIQKELDRRFKETGVQNVYMPIFIPESLLEKEKDHVEGFAPEVAWVTHGG
                     LEPLQERLCVRPTSETLFCDFYARDIHSYRDLPKVYNQWCSVVRWEKTTRPFLRSREF
                     LWQEGHTAHATAEEAEERTIQMLNVYADFCEQVLAIPVIKGQKTDKEKFAGAEATYTI
                     ESLMHDGKALQSGTSHNFGDGFAKAFDIQFTDKDNTLKHVYQTSWGMTTRMIGAIIMV
                     HGDDNGLVLPPRIAPVQIMVVPIQQKKEGVLDKAFELRDRLIKSGFSVKVDDSDKSPG
                     WKFADCEMRGIPLRVEIGPKDMENNQAVLVRRDTHEKTVVSLDELEAKAAELLDTIQH
                     DMLEKARKHRDAHTYTALGWDEFVETINTKPGFVKAMWCGCQECEDKIKEVTGATSRC
                     MPFEQETLSDKCVCCGKPAKKMVYWGRAY"
     gene            7167..8471
                     /locus_tag="ACZPCL_07650"
     CDS             7167..8471
                     /locus_tag="ACZPCL_07650"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR1162374.1"
                     /translation="MKMNIPEPARKIIDRLNEHGYEAYVVGGCVRDMILKREPGDWDI
                     TTSARPEQVKALFTRTLDTGIQHGTVTIMVGKEGYEVTTFRVDGDYTDGRHPDTVTFT
                     PSLEEDLKRRDFTINAMAYNHNTGLVDIFGGREDIDRKVIRCVGNPTERFTEDALRIL
                     RAIRFSAQLGFEIEDATRQAITEIAPNLIHVSKERIQVELTKLLLSDRPQTMKLVYET
                     GISPYVSETFHKMGEMLADMPITVPAKKALRWALFLRKGTPEQSVRILRDLKLDNDTI
                     YQVKVLTGWIERSICGEIALPDGKTDEMCTLETAAELEFMPGIESKPQIRKVMSQMEP
                     ELFDDLLNIKLCLAKEAAKDKDSESVAVRQLERICALRDEIRADGDCISLKMLAVTGN
                     DLIKAGMKPGKEVGQTLHHFLELVLEEPEKNSREYLLGMLKV"
     gene            8706..9074
                     /locus_tag="ACZPCL_07655"
     CDS             8706..9074
                     /locus_tag="ACZPCL_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR1162375.1"
                     /translation="MKLINTEKAPGAIGPYSQGFEVNGIVYTSGQIPVNPADGTVPEG
                     IAAQAEQSCKNVGAILEAAGSGYDKVIKTTCFLADMGDFAAFNEVYAKYFTSKPARSC
                     VAVKSLPKGVLCEIEAIAEK"
     gene            9244..10089
                     /locus_tag="ACZPCL_07660"
     CDS             9244..10089
                     /locus_tag="ACZPCL_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162376.1"
                     /translation="MKNNIVIMNFSGVYEVQGLKAVLEAGKTNNHIISQLDCQDIPGT
                     NCYCDSLAEEEIGKRIVPFGPEGLHFLDSGNYHYLTKLWLELVKEPFELLVFDHHTDM
                     QRPAFGGILSCGGWIREALETNENLKHVILVGPPETAMEETKRELLEDGEELIRKVTW
                     ISEEEFLQNVEKPDGIKKLCGQCKENDLGADEKDPLPLYISIDKDILSESEGKTNWDQ
                     GNVASNQVLHFIDAYMQQTPDKTLIGVDVCGEDPEADSEEVQAVCRETSEKISSFVMT
                     LFKAD"
     gene            complement(10148..11269)
                     /locus_tag="ACZPCL_07665"
     CDS             complement(10148..11269)
                     /locus_tag="ACZPCL_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR1162377.1"
                     /translation="MKPFVDTCIRFFKKETVLCVAGILAFISAFFVHPSAAYIGYIDF
                     RVLSLLFCLMLVVAGFQSIGLFHFLGSALLTKVRTTRQLILVLSLLCFFSSMFITNDV
                     SLLTFVPFGIMILTMTGQQKLLISTIVLQTIGANLGSMFTPVGNPQNLYLASAFSVST
                     GTFLMRMLPLTALSLILLVAAACMLPSASVDIASQPVEEQPEPKKLAVYLALFVVCLG
                     CVSHLIPYPVMLVLVLVTLLFTNRALFHKADYFLLLTFVFFFLFIGNVQNIPAVSDLL
                     HKLIRGRELGAGILLSQCISNVPAAILLSSFTDQVTPLLYGVNIGGLGTLIASLASLI
                     SYRFYVGTENAHTGRYMKVFTLYNVIFLVILALVAWKIL"
BASE COUNT         3348 a         2191 c         2864 g         2904 t
ORIGIN      
        1 ttgtgggctg tcttttcttc ttgccataaa aataacctcc aaactgagta attttatctt
       61 acatcagttt ggaggtttac acaaacttta ggatactccc gcactccttg gtatttccca
      121 gttaaattct atttctttaa ttacagttgt acagaaaatg tttaccggcc tgaacagctt
      181 tacactgctt gcagttccgc tgttcgttct ggcagcaaac attatgaacc gtgctaagat
      241 cagtgagaaa ctgatccagt tctgtaatgg tatcgtagga agattcccgg gcggtcttgc
      301 ctatgcaaat gtattagtat ctatgttatt tgccggaatt tccggttctt cccaggctga
      361 tacagcaggt atcggttcta ttctgatccc ggctatggag gaagaaggtt acaatcagga
      421 aacttccgta ggtgtaactg cggcttcttc taccatcggt atcgttatcc cgccatctat
      481 tcctatggtc gtttactctt ccgtagcagg cgcatccatc ggcgctctgt tcttaggcgg
      541 tgttgttcct ggtatcctga tcggtcttgg acagatgttt gttattttca tgggaaataa
      601 gaaatatcat tatccaaaac aggatccaat gtcatttaaa gactttttaa agctggctat
      661 aaaatgtctt cctccagtat taacacctgt tattattatc ggcggcgtta tcggaggcct
      721 gtgtacagca actgagtccg cagcgatcgc atgtatctat gcgatcatcc ttggtgtatt
      781 cgtatttaag aacctttctt taaaagatgt aaaaccatta ctttatgaca cactgcgtac
      841 cagcgccact tctctgtttg ctctggcaac cgcaaatgct ttgggacagc ttttaagcta
      901 ctacaatgta agtaccagcg ttcagcagat gttcgctacc tacttcccat ataagtggca
      961 gttcttgctg gctgtgatcg gattctattt attccttgga acatttatgg atgctattcc
     1021 tgcaatgatc ctgttcgttc ctgtattaat gcctgttgca accgcatttg gcatcacacc
     1081 agtacagtta ggtctgatca tcgttatcac actggcagtt ggtcaggtaa cacctcctta
     1141 cggtctgtgt ctgctgatcg caggaaggat ttccggaatg agtgtgcaaa agtcgtttaa
     1201 ggctgttatc ccgtatatca tggtttctgt agtagtagta gtgctgatcg catttttacc
     1261 ggatatcgcc tttgcattac cgaaacttat taagccagag tggttttaac atgtaattct
     1321 aaaggaggga tttctcatgg agaaaaagaa ggtagcgatc gtcacattag gtgacagccg
     1381 tcgcgaattt tataaaaaga gagaagcaat tgtagcaagc gagatcgaaa aggttaaaaa
     1441 ggcatttggg gataaatatg acctttatat ctcacctatt gtatttgaca cagaagatgg
     1501 acagaatatt tctgatgaga tcaagaaaca gggcattgaa gctgtgatca tccatgtgcc
     1561 tatctgggca actccatctc tggcattccg cattgcctat ggaacttctt atccggtgct
     1621 gctgttaggc aatttgcagc gcaatacgtc cagccttgta acactgctgg cagttggggg
     1681 tatgttggac cagaccggaa agaaatgcat ccgtgtatct ggtgattatg aagatcccca
     1741 gatccaggaa aaggtagata actttgtaaa aggtgtttat gtggcagaag gcatccgccg
     1801 ttccagctat ggaatgatcg gcggaagaag tattggtatt ggaactacgg tagcagatcc
     1861 gagccagtgg cagagtacct ttggggtaga gtttgaccac tgcgaccagt atgagatcgt
     1921 ataccgtgca aaggcactgg acgaagaacg tgtccagaag catttaaagt gggtaaagga
     1981 aaatgtcccg aggatcgaat acggtgacaa gtttacagag aaaacactgg aattacaggt
     2041 acgcagttat ctggcgttaa aggatatggc tgcggaaaag aaatataact tcatgggcat
     2101 caagtgccag caggatatga gtgaccattt tgttctccag tgtctgggcg tggcattatt
     2161 aaacaaccca tatgacgcag acggcgaaaa gacaccgatt cctacctctt gcgagtgtga
     2221 ctgcgatggt gctatgacca tgcgcatctt aaatctgtgc gcagaaggaa agccttcctg
     2281 tttgctggac atcaagttct ttgacggaaa cgataaggaa tttgtactgg caaactgcgg
     2341 cagcgtggca ccatatttcg ctgatccgga caacaaagaa gaagctatga agaagatcgc
     2401 tttaatgcct catatcttcg gagaagcagg tggtgcatcc atccagttta tctccaaacc
     2461 aggtgatgta acggttgcca gactgttccg cagcaatggg aaatatgtat taggctgctt
     2521 tgaaggaacc acaaagatgt atcctcttga gaagttaaaa gagactacat actgttatcc
     2581 ctatgcattc gtagaagcag aggcagacta cgaaaagttc tacgagaaga tcaactccaa
     2641 ccatctgcat atggtttatg gaaagtatgc gaaggttctg aagatgttct gcgagatcgt
     2701 gggaattgag tatatctgct ttaataagta gtgtttatat aaaaaacaaa tgaatgagtt
     2761 gtaaaattta caggtttaca cagcaacgtc aaaatgttat gatactggta cattatttac
     2821 gaaaacgatg taccggtgtc ctgaggccag aaatcttcct gcaagcaggc ttgcatcaga
     2881 tttctggcct tttggctatg gtattatcat gatgttgtgg caaaaggtat tttgcggtga
     2941 tcaacagaag gtgaggggaa atgtatgaga ctcttatacg agttatcctg gttatgggag
     3001 atgatgggta tcgccatggc tgctgcggtt gtctgtgtgg cttgtgctgc gctgatctgt
     3061 aaggcagcaa agaagaaact cagcaggaaa gtgatagcag ttgttgctgc tgcggcgttt
     3121 gcaggtgctg ttttggcagt tattctaatc gcacggacac ctatgccgct ttgaaaacac
     3181 tgatccactt atacggttta agataaaatt aaccccattt tcccgggaaa tgtgctatac
     3241 tatcagcaca gcagttacca aggagaaagg cggggaaaag tattatgaac aaggtgatta
     3301 cagtgagccg tgaattcggc agcgggggaa gagaactggg tgtgaagctg gcagaggcgc
     3361 tggggattcc cttttatgac aaggaactga tctccatggc agcagatgat attaatattg
     3421 cagaggaagc gttccgcaaa tatgatgagc atgtgatcga ggacgatcct ttggatcaca
     3481 agatccatca ttctttttcg gagttgtata aagtgcctat gtctgaccag atctttgtgg
     3541 cccagtccaa tgtgatccgc agactggctt ctcatggccc atgtgtgatc gtaggacgct
     3601 gcgcagatat gatcctggag gacagcgtga acattttcat atattcaaaa atgaaacagc
     3661 gcataaaacg tctgatggaa ctggaaccgg aagaaggcat caacgaaaaa gacatggaaa
     3721 tgaagatccg tgctgttgac cgcaagcgcc gggattatta ccagtattat actggaaata
     3781 cctggggaag agcccagaat tatcatcttt gcgtggacag cagtttagtt ggcgttgacg
     3841 gctgtttaag agctgctctg gcatatctgg aagatgtgtg caaagatgaa gaaaagccgg
     3901 aagatggcga aaaagaaacc gtataacgga aaaagaaaaa tatcggaatg caattaaaga
     3961 ccgtgaataa ttcccgtcca taagactcat aataagaatg tacaaaggaa gtacagatca
     4021 aaaaacaggt gagtccatgg aaaaaccggg aaagaaactg aagaaatttt ttattataac
     4081 aggaatcaca ggaatggtgt atgcaggtct taaatacctg ctgccacttg tggttcctct
     4141 tttattgggt tatacactgg cactgctcct tcggccgtca gcccggtttt tacagcaccg
     4201 gattacagtg cgtatatttg gaaaaaaatg gaagccatca ctgggactga tcggatcggt
     4261 acagttactg atcttagcgg tgcttggaag ttttctgctg actttgggca tccgccgtct
     4321 gtgggaagaa acgtctactt tgatgaacca gcttcctgta tggctggatg ggctttattt
     4381 ctggatcgag gaaaaatgtt ccggtcttga gaagcggttc ggccttgatc ccggctgggg
     4441 aatccatgtg gcaggaagta tgatcgaagc aggaacaaag tatgtgaaag agaaatcggt
     4501 gccgtccttg atggctggct cttattatac agccggagtt ataggtcgta ttggatttat
     4561 aagcctgttt tctttctgca gcgccatttt aagcttacag gaaatggaag agttaaaaga
     4621 acggcgggac cggtctgttt tcagccggga gttccgtctg atcggtgaca ggctggtaag
     4681 caccgggaaa gtgtggttta aaagccaggg gatcatattt ttacttacct catttttgtg
     4741 tacaataggc atgtttttgg tgggaaatcc atgtcctgtc cttgtgggaa tggggattgg
     4801 tatcatggat gcctttccgg tattaggaac aggaacggtg ctggtgccgt gggcggtcat
     4861 tgagattgcc atgggaagct ggaaaacagc attgatctta acaggtttat atctgatctg
     4921 tacttttatg cggcagttcc tggaagtaca tcttatgagt ggacagatgg ggctttcgcc
     4981 ttttgagaca ctgggggcag tgtatgtggg actgaaactg tttgggatag cagggctgtt
     5041 tctggggccg ctgggattgt tgctgatcga ggatatgaca gaattattga tcacgcagcc
     5101 tatgaacgat gaacagaggc ataatgaaac aaataagtca gctatgatag acggtggtaa
     5161 ccgttttatg tcccattgac aaaaatcccg ttctggcgta aaatcgaaag gaaatgacgt
     5221 aaatcaaaga ggatctatga aattccatga aaatggcgtt gaaggggatc attaagtcgg
     5281 acagcccgtt acagagagag gaacactggt ggaagttcct tacgaaagcg tgacccaacc
     5341 tgccctggag ctctgtatga taaatgcagc gtatttcctg cgttagagga taaacaagag
     5401 ggtttctgca gaggtaaaat gtcatttgcc tcaacatttt tgcaggaact aagaagggtg
     5461 gtaccgccga gtcattcggt cccttgcagt aagttgatgc atgggatcgg atggctcttt
     5521 tgattttatg tactctcgaa acctttcctg cacttgcctt tgcggctgat tttccgccag
     5581 tcgcgcccaa atttcatcaa cgtacgcccc gcgtacgcct cagaaatctg ggcacaactg
     5641 acaaaaaatc ttctcgcaaa atcaagtaca aaaaggttcc gagagtacat ggtttaaaaa
     5701 gaaagaaata aaaaggagaa aaatcatggc agacaacaaa aaaatggtag aagcgatcac
     5761 atccatggac actgactttg cccagtggta taccgatgtg gttaaaaaag cagagctgtg
     5821 cgattatgca agcgtaaagg gctgtatggt catcaagcca gctggatatg cgatctggga
     5881 gaacatccag aaagaactgg accgtagatt taaagagaca ggtgtacaga acgtatacat
     5941 gcctattttc attccggaaa gcctgttaga gaaggaaaaa gaccatgtag aaggtttcgc
     6001 accggaagtt gcatgggtaa ctcatggcgg attagagcca cttcaggaga gactttgcgt
     6061 aagacctact tctgagactc tgttctgcga tttctatgca agagacattc attcttaccg
     6121 tgaccttcca aaggtataca accagtggtg ctctgttgtt cgttgggaga agacaacccg
     6181 tccattcctt cgttccagag agttcttatg gcaggaagga catactgctc atgcaactgc
     6241 tgaagaggca gaggaacgta ccatccagat gttaaatgta tacgctgatt tctgcgagca
     6301 ggttttagcg atcccggtta tcaagggaca gaagactgat aaggagaaat ttgcaggtgc
     6361 agaggctact tacaccatcg agtccctgat gcatgatggt aaggcattac agtccggtac
     6421 cagccacaac ttcggtgacg gctttgcaaa ggcatttgat atccagttta ctgataaaga
     6481 caatacatta aagcatgttt accagacttc ctggggtatg acaacccgta tgatcggcgc
     6541 tatcatcatg gtacatggtg atgacaatgg tctggtactg cctccaagaa ttgctcctgt
     6601 acagatcatg gtagttccga tccagcagaa gaaggaaggc gtattagaca aggcattcga
     6661 gttaagagac cgtctgatca agtccggctt ctctgtaaag gtagacgaca gcgacaagag
     6721 cccgggctgg aagtttgctg actgcgagat gcgtggtatc ccgttaagag tagagatcgg
     6781 accaaaggat atggaaaaca accaggctgt tctggtacgc cgcgataccc acgaaaagac
     6841 cgttgtttcc ctggacgagt tagaagcaaa ggcagcagaa ctgcttgata ccatccagca
     6901 tgacatgtta gagaaggcaa gaaagcacag agatgcccac acttataccg ctttaggctg
     6961 ggatgagttc gttgagacca ttaacacaaa gccaggtttc gtaaaggcta tgtggtgcgg
     7021 atgccaggaa tgcgaagaca agatcaagga agtaaccggc gcaacttcca gatgcatgcc
     7081 atttgagcag gaaactctgt ctgacaagtg tgtatgctgc ggaaagccag ctaagaagat
     7141 ggtttactgg ggacgtgcat attaatatga aaatgaatat cccggaacct gcaagaaaga
     7201 tcattgacag gttaaatgaa catggttacg aggcctatgt agtaggcggc tgtgtccggg
     7261 acatgatcct gaaaagagag ccgggagact gggatattac caccagtgcc agaccggagc
     7321 aggtcaaggc tctctttacc aggacactgg atacaggcat acagcatggc actgtgacca
     7381 ttatggttgg aaaagaggga tatgaggtaa ctacattccg ggtggacggc gattatacag
     7441 atggccgaca cccggatact gttactttta ccccttctct tgaagaagat ttaaagcgcc
     7501 gtgactttac tattaacgcc atggcttata atcacaatac aggcctggtg gatattttcg
     7561 gcggcaggga agacattgac cgtaaagtga tccgctgcgt tggaaatcca acggaacgtt
     7621 ttacagaaga tgccctgcgt atcctgcggg ctatccgttt ttctgcccag ctgggatttg
     7681 agatcgaaga tgccacacgg caggcgatca cagaaatcgc tccaaatctg atccacgtaa
     7741 gcaaagaacg tatccaggtg gaactgacca agctgctttt aagtgaccgg ccacagacta
     7801 tgaagctggt atatgaaaca ggcatcagcc cctatgtttc tgaaacattt cacaaaatgg
     7861 gagaaatgct tgcagatatg ccgattactg ttcctgcaaa aaaggccctt cgttgggctt
     7921 tatttttaag aaaaggaaca ccggagcagt cagtgcgcat tttacgggat ctgaaactgg
     7981 ataatgatac tatttaccag gtaaaggtgc tgactggttg gatcgaaaga tccatttgcg
     8041 gggagatcgc tcttccagat ggaaaaacag atgaaatgtg tacgctggaa acggcagcag
     8101 aactggaatt tatgccaggt atcgaaagca agcctcagat ccgtaaagtc atgagccaga
     8161 tggaaccgga attatttgat gatctgttaa atattaagct gtgtctggca aaagaggcag
     8221 caaaagacaa ggatagcgaa agtgtggcgg taaggcagtt agagcggatc tgtgcattgc
     8281 gggatgagat ccgcgcagac ggtgactgca tttccttaaa aatgcttgct gttaccggaa
     8341 atgacctgat aaaagccgga atgaagccgg gaaaagaagt ggggcagaca ttgcatcatt
     8401 ttctggaact ggttttggaa gagccggaga agaacagcag agaatacctt cttggaatgt
     8461 taaaagtata aaacagttaa tagatttgca aaatagcaca gagtatattt atagataaat
     8521 agtcagcttt taaatttatt tgttactatg ttttacaaaa ttatttaggt taataaaaaa
     8581 taatcatgta tgtttttact taattttaat gatgtatgtg tataaaaaca ttgcaatatt
     8641 tacaaaggtt ggctataata ttggatacat gatgtgaaca tcacagttca aaggaggatt
     8701 tgaaaatgaa attaatcaac acagaaaaag caccaggcgc aatcggacct tattctcagg
     8761 gttttgaagt aaacggtatt gtttatactt ccggtcagat cccggtaaat ccagctgacg
     8821 gcactgtacc agaaggcatc gctgcacagg ctgagcagag ctgcaagaac gtaggagcta
     8881 tcctggaagc agcaggaagc ggctatgaca aagttatcaa gaccacctgc ttcttagcag
     8941 atatgggcga tttcgcagca ttcaacgaag tatacgctaa gtactttact tctaagccag
     9001 ctagaagctg cgtagcagtt aagtctcttc caaagggcgt tctttgcgag atcgaagcaa
     9061 tcgctgagaa ataagtaata gttgtgaaat aaaaatggct gcagtttctt agaagctgca
     9121 gctttttttc gcttaaaatg gaagtgggaa acgcgcccgg gggcgcgttg caggctgtga
     9181 agcgtttgga gcagaacgga aagcggtgca aggccattta tcaggtacgg agaggtttta
     9241 aatatgaaga ataacattgt gatcatgaat ttcagtggag tttacgaagt gcagggccta
     9301 aaggcagtgc tggaggcagg aaaaacaaat aatcatatta tttcccagtt ggactgccag
     9361 gacattccgg ggactaactg ctattgtgat agcctggcag aggaagagat cggaaagaga
     9421 attgtcccat ttggtccgga aggactgcat tttttagact ccggcaatta ccattatctg
     9481 acaaaactgt ggctggaatt ggtaaaggaa ccttttgaac tgttagtatt tgaccaccat
     9541 acggatatgc agagaccggc ctttggcggc attttatcct gcggtggctg gatccgggaa
     9601 gcactggaga ccaatgaaaa tttaaaacat gtaattctgg tgggaccacc ggaaactgcc
     9661 atggaagaaa caaaaaggga gcttttggaa gacggggaag aattgatcag gaaagtaacc
     9721 tggatctctg aggaagaatt tcttcaaaat gtggaaaagc cggatggaat aaagaaactt
     9781 tgtggacagt gcaaagaaaa tgatttagga gccgatgaaa aagatccatt gccactgtat
     9841 atttccattg ataaagatat tttatcagag tctgaaggaa agaccaactg ggatcaggga
     9901 aatgtggctt caaatcaggt tttacatttt atagatgcgt atatgcagca gacaccggat
     9961 aaaacgctta ttggtgtgga tgtatgcggg gaagacccgg aagcagacag tgaggaagtt
    10021 caggcggtct gccgggaaac cagtgaaaag atcagcagct ttgtaatgac attgtttaaa
    10081 gcggattaaa aagtaaaaat ctgccagtta tccccgtcgg atcagacggg gatttttgct
    10141 gtatatttta aagaattttc catgccacca aagccaggat gaccagaaag atcacattat
    10201 aaagtgtaaa taccttcata tagcgcccgg tatgcgcatt ttctgtcccc acataaaaac
    10261 gataggagat cagactggct aaggatgcga tcagagttcc aaggccgccg atattgaccc
    10321 cgtacagcag aggtgtgacc tggtctgtaa agcttgaaag caggatggca gcaggtacat
    10381 tgctgatgca ctggcttaaa aggatcccag cgcccagttc acgtccccgg atcagcttgt
    10441 ggagcaggtc agatactgct gggatgttct gcacatttcc gatgaacaga aaaaagaaaa
    10501 caaacgttaa aagcagaaag taatctgctt tatggaacaa tgcccggttg gtaaaaagca
    10561 aagttacgag gacgagaacc agcataacag gatatgggat caggtgggat acgcatccca
    10621 gacagaccac aaaaagagcc aggtaaacag ccagcttttt cggttctggt tgttcttcta
    10681 ctggttggga agctatgtct acagatgcag agggaagcat gcaggctgcc gcaaccagca
    10741 aaatcagaga aagggctgta aggggaagca tacgcatcaa gaaagtccct gtggaaacag
    10801 aaaaggcaga ggccagatac aggttctggg gattacctac aggtgtgaac atgcttccta
    10861 aatttgctcc gatagtctgc aaaacaatgg tggagatcaa cagtttttgc tgtccggtca
    10921 tagtcaggat cataatgcca aaaggcacga atgtaagcag cgatacatca ttggtgatga
    10981 acatgctgga aaagaagcac agcagggaaa gcaccagtat taattgccgt gtagtccgta
    11041 cttttgtaag cagtgcagag ccgagaaaat gaaaaagacc aatactctga aatccagcca
    11101 ccaccagcat aagacagaaa agcagggaaa ggacccggaa gtcgatatac cctatataag
    11161 cggcagaggg atgtacaaag aatgcggaaa taaaggctaa aattccggca acacataata
    11221 cagtttcttt tttaaaaaag cggatacagg tatctacaaa tggtttcata atcaacatgt
    11281 cctttacaat attcaatcaa caaattc
//
LOCUS       DBMDUA010000082        11265 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2901_length_11265_cov_2.222680, whole genome shotgun sequence.
ACCESSION   DBMDUA010000082 DBMDUA010000000
VERSION     DBMDUA010000082.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11265)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11265)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11265
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2901_length_11265_cov_2.222680"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            274..1578
                     /gene="ftsZ"
                     /locus_tag="ACZPCL_07670"
     CDS             274..1578
                     /gene="ftsZ"
                     /locus_tag="ACZPCL_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR1162378.1"
                     /translation="MLEIKINESENAARIIVVGVGGAGNNAVNRMIDENIAGVEFIGI
                     NTDKQALQFCKAPTAMQIGEKLTKGLGAGARPEVGEKAAEESSEEISQALKGADMVFV
                     TCGMGGGTGTGAAPVVAKIAKDMGILTVGVVTKPFRFEAKARMQNALNGIECLKQAVD
                     TLIVIPNDRLLEIVDRRTTMPDALKKADEVLQQAVQGITDLINVPGLINLDFADIQTV
                     MIDKGIAHIGIGKAKGDDKAIEAVKQAVSSPLLETTIEGASHVIINISGDISLIEAND
                     AASYVQELAGDDANIIFGAMYDENAQDEASITVIATGLDEHNATSSVSKVMSGLNSYK
                     AQPAEKSASNEAAATETAAPTFTKPAQPAFNRTFTPNTQQTTGYTIPKFNATPTFNAG
                     NAGSQPAEKPAAPAQERTTPSFRPAANKEMQINIPDFLKNKR"
     gene            1773..2600
                     /locus_tag="ACZPCL_07675"
     CDS             1773..2600
                     /locus_tag="ACZPCL_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HIR1162379.1"
                     /translation="MVYRVYIDVIFAVNLFMDLAVLAMLNRLFSYRADLKHMIRGAVL
                     GAVWACVLAVFPETPLLLQMFGTYVAVGSLMAVTAYGIRSRKELIRAVGGIYLISVVL
                     GGVMLALSESSLGREVANWMPGRFSIRFFLAAGAFCFCFWVGDLLRITGNRKTEQKYL
                     RNVTLLYEGQKIKTRGLIDTGNHLTAPASGIPVHVATAELMKRLCPVVKGVMYVPYRC
                     VGSSGILPAVRIDEMKVEGEKDSFVIPKPWIAITKGSLSPCDEYEVLIQKNDEGMQV"
     gene            2613..3353
                     /gene="sigE"
                     /locus_tag="ACZPCL_07680"
     CDS             2613..3353
                     /gene="sigE"
                     /locus_tag="ACZPCL_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HIR1162380.1"
                     /translation="MMIKVAVPNRFQLKTVSRFRTVLMPGQGEVHYIGGADILPPPLD
                     AREEGKMIGMLGTEEDGKARSALIEHNLRLVVYIAKKFDNTSVGVEDLISIGTIGLIK
                     AINTFKPDKNIKLATYASRCIENEILMYLRRNNKTKLEVSIDEPLNVDWDGNELLLSD
                     ILGTDEDVIYHGIEDEIEKNLLNTAISRLAPRERKIVELRYGLTNEDGEEMTQKEVAD
                     LMGISQSYISRLEKKIMKRLKKEIVRFE"
     gene            complement(3390..4304)
                     /locus_tag="ACZPCL_07685"
     CDS             complement(3390..4304)
                     /locus_tag="ACZPCL_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR1162381.1"
                     /translation="MELNDRRFAVLIDADNVSPKYIKYILNEVSDLGVATYKRIYGDW
                     TDTEKKSWKNVLLDWSVNPIQQYSYTTGKNSTDSAMIIDAMDILYSGNVDGFCLVSSD
                     SDFTRLAQRLREAGMFVMGIGEQKTPKPFRAACDAFKILEIISSEDTPEDVVPVKVST
                     DEIEKAITKLLIENNSQNQPVNLAKVGNFLTKRFSDFDVRNYGYSKLSTFLESLNSND
                     FHVVKLHNGYFVQEKTASVPKSEIEKEICRLIKNNKGRVDNLSIIHEDLKKTFPSFDV
                     KQYGFSRLSSFIRSFNTFKIKDNVVQMR"
     gene            4524..5957
                     /locus_tag="ACZPCL_07690"
     CDS             4524..5957
                     /locus_tag="ACZPCL_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HIR1162382.1"
                     /translation="MKKLVSLLEAILLMVMAFPLTALAGQEGTQIISPVFCIPFAGLL
                     LCVAIMPLVKAEWWESHQPHAVVFWSVLFVIPFAIFFGAGEATEKVLECIIDDYLTFI
                     VLLFGLFCVAGNITLEGDLAGSPRINVGLLLLGTMLSSWVGTTGASMLMVRPVIKMNA
                     WRKRRSHIMVFFIFLISNIGGCLTPIGDPPLLMGFMRGVPFFWSLHLLPILLFNVVVL
                     LTVFYFLDRRAYRKDIAEGRKPDISKPGTEVHILGLHNLIFLVMIVAAVILSGTLPGM
                     AAFKDASGAVKGIPVFGDVKLTYPALIEVVIILLAAFLSFKTTDAEIRRKNHFTWGAI
                     REVAVLFIGIFITMQPALMILKAKGAELGITEPFQMFWATGFLSSFLDNTPTYLVFLT
                     TAGSLGFTEGMQTILGTVPVAMLEAISCGAVFMGANTYIGNAPNFMVKAISDENGIKM
                     PSFFGYLMWSIAFLVPVFLLDMLVFFL"
     gene            5973..6992
                     /locus_tag="ACZPCL_07695"
     CDS             5973..6992
                     /locus_tag="ACZPCL_07695"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1162383.1"
                     /translation="MEYTKENLQELAESIYDLDAEVYAQLGSSLGRVFNRDREGCVSD
                     MVQLMLRKDSHQLRSELALISNMARTIEDKGTRSMIMTEYNRILKEVIALPTSFASGD
                     ILDAKAAGLNALQFKARFGKDNHRIICISRTYGCGGNEIGFMLADKLKINYYDAEIFS
                     AVLKRLQAEQDERIKDSSAYPDKANLEVAFAAPKRMTLRDHARQFSRYHGLSKRDAVF
                     FNQSDLICDMAKKEDFIVMGRCADVILTNNHIPHISIFITAPFERRVQRAMEMHPDFN
                     EKKAKHMLRQLDRQHESYYRFYTGRRWGNADNYDLCINSSAYGIEGSVDFILRMLKNS
                     EEKEA"
     gene            complement(7080..7856)
                     /gene="sigG"
                     /locus_tag="ACZPCL_07700"
     CDS             complement(7080..7856)
                     /gene="sigG"
                     /locus_tag="ACZPCL_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HIR1162384.1"
                     /translation="MSGYKVEICGVNTAKLPLLSSKEKEELFEKILKGDKDARERYIK
                     GNLRLVLSVIQRFSGSQENLDDLFQIGCIGLIKAIDNFDITQNVKFSTYAVPMILGEV
                     RRYLRDNNAIRVSRSLRDTAYKALCAREQLTRLHAKEPTIMEIAQEIGISGEDITYAL
                     DAIQTPVSLYEPVFTDGGDPLYVMDQISDKKNREENWVEDLSLLEAMKRLPDRERHII
                     DMRFFEGKTQTEVAQEIHISQAQVSRLEKNALRSMRTYLA"
     gene            8047..8478
                     /locus_tag="ACZPCL_07705"
     CDS             8047..8478
                     /locus_tag="ACZPCL_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HIR1162385.1"
                     /translation="MILIVCVDDNKGMMFNHRRQSQDRVLRRHILDRVGDAKLWMNGY
                     SAKMFEKDESEQVRKCLIVDEDFLKKAAKGEYCFAESDPCGKDMFPDTTQVDSVILYK
                     WNRVYPADQYFAMDLSGWKLVETVEFPGSSHEKITEERYER"
     gene            8475..>9396
                     /locus_tag="ACZPCL_07710"
     CDS             8475..>9396
                     /locus_tag="ACZPCL_07710"
                     /EC_number="3.1.30.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HIR1162386.1"
                     /translation="MRKMKYWVLGLAMSVSLLVGACGQAPATGTSAAVKETTEAVTES
                     VTVEAVQTPKAAAAQTGNSGAFDLSAIPAYSGQPYVAVNNNVPFFFDADLTDVSFESY
                     GDLDALGRCSVAYASVGTDTMPTEKRGNIGQVKPTGWHTVKYDFVDGKYLYNRCHLIG
                     YQLTAENANEKNLITGTRYLNVQGMLPFENMTADYVKETENHVLYRVTPVFEGNNLVA
                     AGVLMEAESVEDKGEGVEFCVFVYNAQPGVTIDYATGDSWLDENGTGNQQAAAKETKT
                     VVETEIQAEKQTQAETTFSCAATDSTRQKSK"
     assembly_gap    9397..9406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9407..9624
                     /locus_tag="ACZPCL_07715"
     CDS             <9407..9624
                     /locus_tag="ACZPCL_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162387.1"
                     /translation="LISFIAQIGNETTQAPAKETSTYILNTNSKKFHKPGCSAASQIK
                     AANKDEFTGTRDEVIAKGYEPCKKCNP"
     gene            complement(9755..10522)
                     /locus_tag="ACZPCL_07720"
     CDS             complement(9755..10522)
                     /locus_tag="ACZPCL_07720"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR1162388.1"
                     /translation="MILAIDMGNTNIVLGGIDEKQTYFIERVTTDQGRTDTEYAIHFK
                     NILEMHHIQLSDIEGAILSSVVPPLNTTILNAVEKAVGIRPLLVGSGMKTGMNILMDN
                     PKSVGSDMIVDVVAAIHEHPLPLVVIDMGTATTLSVADKKGNYIGGVILPGLRVSLNS
                     LSGKTAQLPYISLEVPNKVIGKNTIDCMRAGIVFSNVDMIDGILDRMEKELGETPTVI
                     ATGGLARFITPLCRHEIIFDDALLLKGLLILYRKNTQ"
     gene            10606..>11265
                     /gene="coaBC"
                     /locus_tag="ACZPCL_07725"
     CDS             10606..>11265
                     /gene="coaBC"
                     /locus_tag="ACZPCL_07725"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR1162389.1"
                     /translation="MLKGKTILLGVTGSIAAYKIAYLASALVKAGAKVHVLMTQNATN
                     FINPITFETLTGNKCLIDTFDRNFQFSVEHVSLAKEADVFMVAPASANVIGKLAHGIA
                     DDMLTTTFMACTCPKIISPAMNTRMYENPITQDNLKILEHYGMEVIRPASGYLACGDT
                     GAGKMPEPETLFEYILRTVGYPKDMVGKKVLITAGPTREAIDPVRYITNHSTGKMGYA
                     IA"
BASE COUNT         3261 a         2257 c         2752 g         2985 t
ORIGIN      
        1 aagggaaccc tggaactgga tggatattca gatacgggaa atgacgggac cattcctttt
       61 aaattaaaat aaagtcaact gtctaaccgt gcttatgtgc aagcacaacg ctcggttaaa
      121 cgcttgacaa tgcttaaaaa agtaaattca gggttgattt tttctggaaa ataaaatata
      181 gtattagtta ggctagaaat cagaaacatt aattatagaa aaaacacagt cccatgttct
      241 gtgagcaggg ttttaagaag gaggacaagt atcttgttag agattaaaat aaatgagtca
      301 gagaatgctg caagaattat cgtagtaggt gtaggtggcg caggaaacaa cgctgtaaac
      361 cgcatgatcg atgaaaatat cgcaggtgtt gaatttatcg gtatcaatac tgataagcag
      421 gcactgcagt tctgtaaggc tcctaccgct atgcagattg gtgagaagct gaccaaggga
      481 ttaggtgcag gtgcacgtcc ggaagttggt gaaaaggcag cagaagagag ctctgaggag
      541 atctcccagg cattaaaggg cgctgacatg gtattcgtta cctgtggtat gggtggcggt
      601 accggtactg gtgcagctcc tgttgttgca aagatcgcaa aggatatggg tatccttact
      661 gtcggtgttg taacaaagcc tttccgtttt gaagcaaaag cacgtatgca gaatgcgtta
      721 aatggtattg aatgtttaaa gcaggcagta gatactctga tcgttattcc aaatgaccgt
      781 cttcttgaga tcgtagaccg ccgcacaact atgccggatg ctcttaagaa agcagatgaa
      841 gttcttcagc aggcagttca gggtattacc gacctgatca atgttcctgg tctgatcaac
      901 cttgactttg cagatatcca gactgttatg atcgataagg gtattgccca tatcggtatt
      961 ggtaaggcaa aaggtgatga caaggccatc gaggctgtta agcaggctgt atccagtcct
     1021 cttctggaga ccactattga gggtgcaagc catgtgatca tcaatatttc cggtgacatc
     1081 agcctgatcg aagcaaatga cgcagcaagc tatgttcagg aactggctgg tgatgatgca
     1141 aacattatct tcggtgctat gtatgatgaa aatgcacagg atgaagcaag cattactgtt
     1201 attgctaccg gactggacga gcataacgca acttcttctg tcagcaaggt tatgagcgga
     1261 cttaacagct ataaggcaca gccagcagag aagagtgctt caaacgaagc agcagcaact
     1321 gaaactgcag ctccaacctt tacaaagccg gcacagccag catttaaccg cacatttaca
     1381 ccaaatacac agcagacaac cggatatacc atccctaaat tcaacgctac tccaactttc
     1441 aatgcaggaa atgcaggaag ccagccagca gaaaagccag cagctccggc acaggaaaga
     1501 accactcctt ccttccgccc ggcagcaaac aaagagatgc agatcaatat cccggatttc
     1561 ttaaagaata agagataatt tggatccaca aatataaaac ttcaaaaaga attttaaaag
     1621 acctttccta caggaaaaaa gcctgtggga gaggtctttt tttgtcgaaa aaaacaacca
     1681 tttctccccc ttacttcgac agcttttgca cttgagaatg attataatgt acctcagatg
     1741 aaaacacagg aggtggaaaa aagctaggaa agatggtgta cagagtttac atagatgtca
     1801 tatttgcagt gaacctgttt atggatctgg cggtacttgc tatgttaaac cgactttttt
     1861 cttaccgcgc agaccttaaa catatgataa gaggagccgt tttaggagct gtatgggcct
     1921 gtgtcctggc tgtgtttcca gagactcctc tgcttttgca gatgtttgga acctacgtgg
     1981 cagttggaag ccttatggca gtaactgctt atgggatccg aagcaggaaa gagctgatcc
     2041 gggcagtagg cgggatctat ctaatatccg tagtcttagg aggtgttatg ctggcgcttt
     2101 ctgagagcag tctgggaagg gaagttgcaa actggatgcc tggaagattt tccatccgct
     2161 tttttctggc agcaggagct ttttgtttct gcttctgggt aggagatctt ttaaggatca
     2221 caggaaacag aaaaacggag cagaaatatc tgcggaatgt cacattgctt tatgagggcc
     2281 agaaaataaa gaccagggga ctgatcgata caggaaacca tcttacagct cctgcaagcg
     2341 gtattccggt ccatgtggca actgcagagc tgatgaaacg gctctgtcct gtggtaaaag
     2401 gggtcatgta tgtaccttac cgctgcgtag gaagcagcgg gatccttccg gctgtaagga
     2461 tcgatgaaat gaaggtagaa ggggagaaag acagttttgt gatcccgaaa ccctggatcg
     2521 ccattacaaa aggaagtctg tctccctgtg acgaatatga agtgctgata cagaagaatg
     2581 atgaaggcat gcaagtgtag gaggaatgga tcatgatgat aaaagtggct gtaccaaatc
     2641 gttttcagtt aaagactgtt tccaggttca ggacggttct tatgccggga cagggggaag
     2701 tacattatat tggaggggct gacattttac cgccaccttt ggatgcaaga gaagaaggaa
     2761 aaatgatcgg tatgctggga acagaagagg atgggaaagc ccgttctgcc ctgatcgagc
     2821 ataatctccg cctggttgta tacattgcaa agaaatttga caacaccagc gtaggagtgg
     2881 aagacctgat ctccattggg accattggac tgatcaaggc gatcaatacc tttaagccgg
     2941 ataagaacat aaaactggct acctatgcgt caagatgtat tgaaaatgag atcctcatgt
     3001 atttaaggcg gaataataaa accaagctgg aagtttccat tgatgagcct ttaaatgtag
     3061 actgggatgg aaatgagctt ctgctttccg atattttagg aacagatgaa gatgtgatct
     3121 atcatgggat tgaagatgag atcgagaaaa atctgttaaa tacagctatc agccggctgg
     3181 ctccaagaga gcgcaagatc gtggaactgc gctacgggct tacaaatgaa gacggggaag
     3241 agatgacaca gaaagaagtg gcagacctga tggggatttc ccagtcctac atatccaggc
     3301 tggagaaaaa gatcatgaag cggttaaaaa aagaaattgt ccgctttgag taataaagtt
     3361 gttttataaa aatgtggtgc agaggcggat caacgcatct gcaccacatt gtctttgatc
     3421 ttaaaagtat tgaaactgcg gataaagctg gataagcggc tgaaaccgta ctgctttaca
     3481 tcaaaggaag gaaatgtttt tttcaagtct tcatggataa tgcttaagtt atccacacgt
     3541 cccttattgt ttttgatcag acggcagatc tctttttcaa tttctgattt tggaacagaa
     3601 gcggtctttt cctggacgaa atatccatta tgtagtttta ccacatggaa atcattgctg
     3661 tttaaacttt ccaaaaatgt ggaaagtttg gaataaccgt aattgcgcac atcaaaatcg
     3721 gaaaagcgtt tggtaaggaa gtttccgact tttgccaggt ttacaggctg gttctggctg
     3781 ttgttttcaa taagtagttt ggtgatggct ttttcaatct catctgtact tacttttaca
     3841 gggacaacat cctccggcgt gtcttcggaa gaaatgatct ctaggatctt aaaagcgtcg
     3901 caggcagcgc ggaacggctt tggcgttttc tgctcaccga tccccataac aaacatccct
     3961 gcttctctta aacgctgtgc cagacgggta aagtcagagt cactggatac caggcagaaa
     4021 ccgtctacat ttccggaata gaggatatcc atagcgtcaa tgatcatggc agagtctgta
     4081 gaattttttc cggtagtata gctgtattgc tggatcgggt ttacagacca gtctaagagt
     4141 acatttttcc agcttttctt ttctgtatct gtccagtcgc cgtagatcct cttgtaagta
     4201 gccactccca gatcggaaac ctcgttcaga atgtatttaa tatatttagg tgaaacattg
     4261 tcagcgtcaa taaggaccgc aaatcttcga tcattcagtt ccataggtta aattcttcac
     4321 tttctgtggg taattataat gtcagcgata aaatgtatgt tattttaaac atgatagtac
     4381 cagtttatca tacttaaaga agaggggcaa gatgagaaaa tacgtgaata aaatatcaat
     4441 tcttgcattt atcttaaaaa gtgctacaat tcaacaatgc gaagaatgaa tgtataaatg
     4501 taaatacaaa ggaggagatt attttgaaga agttggtttc gttgctggaa gccatactgc
     4561 ttatggttat ggcatttcca ctgacagcgt tagccggaca ggaaggaacg cagatcattt
     4621 cacctgtgtt ctgtattcct tttgcaggtc ttttgctgtg tgtagccatt atgcctcttg
     4681 taaaggcaga atggtgggaa agccaccagc ctcatgcagt ggtattctgg tctgttttat
     4741 ttgtcattcc atttgcaatc ttttttggcg caggagaagc aactgaaaaa gtgctggaat
     4801 gcattataga tgattatctt acatttattg tattgctgtt tggcctgttc tgcgtagcag
     4861 gtaatattac cctggaagga gatctggcag gttctccaag gatcaatgta ggacttctgc
     4921 ttttgggaac catgctttcc agctgggtag gaactacagg tgccagtatg ttaatggtgc
     4981 gtcctgtgat caagatgaac gcatggagaa agagaagaag ccacattatg gtattcttta
     5041 ttttcctgat ctccaatatt ggcggctgcc ttacacccat cggtgacccg cctctgttaa
     5101 tgggctttat gcgtggagtt ccgtttttct ggagcctgca tttgctgcct atcctgctct
     5161 ttaacgtagt ggtcctgctg accgtatttt atttcctgga ccgcagggct tacagaaagg
     5221 acattgcaga aggaagaaaa ccagacatca gcaaaccagg tacagaggta catatcctgg
     5281 gacttcacaa tctgatcttt ctggttatga tcgttgctgc cgttatctta agcggaactt
     5341 taccgggaat ggcagcattt aaagatgcaa gcggagctgt aaaaggcatt cctgtatttg
     5401 gggatgtaaa gcttacctat ccggcactga tcgaagtagt catcatcctg ttagctgcat
     5461 tcttatcctt taaaaccacg gatgcagaga tccgccgcaa gaaccacttt acctggggcg
     5521 ccatcaggga agtagcagtg ctgtttattg gtattttcat cactatgcag ccggccctta
     5581 tgatcttaaa ggcaaagggt gcagagcttg gtatcacaga gccgttccag atgttctggg
     5641 caaccggttt cctgtcaagc tttctggaca atacacctac ctatctggta ttccttacga
     5701 cagcaggttc tctgggattt acagagggaa tgcagaccat tcttggaact gtaccggtgg
     5761 caatgctgga ggcaatttcc tgcggtgccg tatttatggg ggccaatacc tatattggaa
     5821 atgcgccaaa ctttatggta aaagcaatct ccgacgaaaa cggtatcaag atgccttctt
     5881 tcttcggcta tctgatgtgg tccattgcat ttctggttcc ggtcttttta ctggacatgc
     5941 ttgtattctt tttataagga ggggaacgta tcatggaata taccaaggaa aatctccagg
     6001 aattagcgga gagtatatac gatctggatg cggaagttta tgcccagtta ggttcttctc
     6061 tgggccgtgt tttcaaccgt gaccgtgaag gctgtgtcag tgatatggta cagctgatgt
     6121 taagaaaaga ctctcatcag ctgcgaagtg aactggcact gatcagcaat atggcccgta
     6181 ccattgaaga taagggaacc cggtccatga tcatgacgga atataaccgt attttaaagg
     6241 aagtaatagc gcttcctacc agttttgcaa gtggggatat cctggatgcg aaagcagcag
     6301 gcttaaatgc actgcagttt aaggcacgtt ttggtaagga caatcaccgg atcatctgca
     6361 tcagccgtac ttatggctgc ggcggtaatg agatcggttt tatgctggcg gataaattga
     6421 agatcaatta ctatgatgca gagatcttct ccgctgtttt aaaacgtctt caggcagaac
     6481 aggatgaaag gattaaggac agcagtgcat atccggataa ggcaaacctg gaagtggctt
     6541 ttgcagcacc aaagaggatg accttaagag atcatgcaag acagttcagc cgttaccatg
     6601 gtttatccaa acgggatgct gtattcttta accagagtga tctgatctgt gatatggcaa
     6661 aaaaagagga ttttatcgtt atgggacgtt gcgccgatgt gatccttacc aacaaccata
     6721 ttcctcatat cagcattttt attacagcac cttttgagcg gcgtgtgcag cgtgcaatgg
     6781 aaatgcatcc ggattttaat gaaaagaaag caaagcacat gctgcggcag ttagaccgcc
     6841 agcatgagag ttattaccgg ttctacacag gtcgcagatg gggcaatgca gataactatg
     6901 acctgtgtat caacagttct gcttatggaa ttgagggctc tgtggatttt attttaagga
     6961 tgttgaaaaa ttcagaagag aaggaagcct gatttcaagg cttcccggca gtataaagca
     7021 tagactctga tcatcaggtc cggtttgggg acagaggtgg cagcaggaga aatcacagat
     7081 caggccagat aggtacgcat ggaacgcagg gcattttttt caagccttga gacctgggcc
     7141 tggctgatgt ggatctcctg tgccacctct gtctgtgttt taccttcaaa aaaacgcatg
     7201 tcgataatgt ggcgttcccg gtccggaagc cgtttcatgg cttctaaaag agaaaggtct
     7261 tccacccagt tttcttcccg gtttttctta tcgctgatct gatccataac ataaagagga
     7321 tcaccgccgt ctgtgaaaac cggttcatag agactgaccg gtgtctggat ggcatctaag
     7381 gcataggtga tatcttcccc ggagatgccg atctcctgtg cgatctccat gatcgttggc
     7441 tcttttgcgt gaagccttgt aagctgttcc ctggcacaga gcgccttgta agcagtatca
     7501 cgcagggagc ggctgacccg gatggcatta ttatcccgca gataccttct cacctcccct
     7561 aagatcattg gtacggcata agtggaaaat ttaacattct gtgtaatatc aaaattgtca
     7621 atggctttga tcaggccgat acatccgatc tggaacagat catctaaatt ctcctgactg
     7681 ccggaaaaac gctggatcac acttagcacc agccgcaggt ttcctttaat gtagcgttcc
     7741 cttgcatctt tatccccttt tagtatcttc tcaaacaatt cttctttttc tttgctgcta
     7801 agaagcggca gctttgctgt attcactccg cagatctcaa ctttatagcc ggacatattg
     7861 tgtctacctc cgttatgata gcttcgatga aaaacgccgt tataacggaa tgatgcacga
     7921 aatgagggga ttttatacct ggaaaaagaa attgcgtgga tcagaagaaa caattataat
     7981 ataagagtgt gtgcgaaaat gaaaaattcc gagaggatat aaaacagaaa cggggtgtaa
     8041 agtcgtatga tcctgatcgt gtgtgtggat gacaacaagg gcatgatgtt taaccatcgc
     8101 cgccagagcc aggaccgggt gcttcgtcgg catattctgg acagagtggg agatgcaaaa
     8161 ctgtggatga atgggtacag cgcaaaaatg tttgagaaag atgaaagcga acaggtacga
     8221 aagtgcctta tagtggatga agactttctt aaaaaggctg caaaaggaga atattgtttt
     8281 gcagagtcag atccctgtgg caaagacatg tttcctgata caacccaggt tgattcagtg
     8341 atcctgtaca aatggaaccg ggtatatcct gcagatcagt attttgctat ggatctgtcc
     8401 ggatggaaac tggtggaaac agtggaattt ccaggttctt cccatgaaaa gataacagaa
     8461 gagaggtacg aaagatgaga aaaatgaaat attgggtatt gggactggca atgtctgtgt
     8521 cccttttagt tggcgcctgt ggacaggcac ctgctacggg gacgtccgca gctgtaaaag
     8581 agacaacaga ggcagttaca gaaagcgtga ctgtagaagc agtccagaca ccaaaggcgg
     8641 cagctgccca gactggaaac agcggggcat ttgatctgtc tgcgatcccg gcttattcgg
     8701 gacagccgta tgttgcagtt aataacaatg ttcccttctt tttcgacgca gatctgaccg
     8761 atgtttcttt tgaaagttat ggggatctgg atgcgttggg acggtgcagt gtggcatatg
     8821 cttctgtagg aacggatacc atgcctacag agaaacgtgg aaatatcggt caggtaaagc
     8881 caacgggctg gcatacagta aaatatgact ttgtagatgg caaatacctg tataaccgat
     8941 gccatctgat cggataccag ctgacagcgg aaaatgcaaa tgagaaaaac ctgatcacag
     9001 gaaccagata cttaaatgtc cagggaatgc tgccttttga aaatatgaca gccgactatg
     9061 taaaagaaac agaaaaccat gttttatacc gtgtgacccc tgtttttgag ggtaataacc
     9121 tggtagcagc aggtgtgctt atggaggcag agtcagtaga ggataaagga gaaggcgttg
     9181 agttctgcgt atttgtctat aatgcgcagc cgggagtaac gattgattat gctaccggtg
     9241 acagctggtt agatgaaaat ggtacgggaa atcagcaggc tgctgcgaag gaaacaaaaa
     9301 cagtagtgga gacagaaatc caggcagaaa aacagaccca ggctgaaacc accttttcct
     9361 gcgccgcaac agacagtacg agacagaaaa gcaagcnnnn nnnnnnttct gataagtttc
     9421 atagcccaaa tcggcaatga aaccactcag gcaccagcaa aagagacttc cacgtatatc
     9481 ctgaatacta attcaaagaa attccataaa ccgggctgtt ccgctgcgtc acagataaaa
     9541 gcggcaaaca aagatgagtt tacaggaacc agggatgaag tgatcgccaa gggatatgaa
     9601 ccttgtaaga aatgtaatcc ataggggaac gaagatcttg taaaagaatg atcagatgta
     9661 cccagcataa cacagctgcg gggagaattc aatcataaaa ataaataaat taaaattatt
     9721 tcaggagcgg cagaaatacc gctcctgaat tgttttattg ggtattcttc cggtaaagga
     9781 tcagaagtcc tttaagcagc agcgcatcat caaagatgat ctcatgtctg caaagagggg
     9841 tgataaaacg ggccagaccg ccggttgcga tcacagtagg tgtttctccc agttcttttt
     9901 ccatacggtc taagataccg tcgatcatgt ctacattgct gaaaacaata ccggctctca
     9961 tacagtcgat ggtgttcttt ccaatgacct tatttggcac ttccaggctg atgtaaggaa
    10021 gctgggccgt ttttccgctt aaggagttta aggaaacacg caatcctggt aaaatgacgc
    10081 cgcctatgta attgcccttt ttatcagcaa cacttaaggt agtagcagtt cccatgtcga
    10141 tcaccaccaa tggaagagga tgttcatgga tagcagctac tacatctacg atcatgtcgc
    10201 ttcctacgct tttagggttg tccataagga tgttcatgcc ggttttcata ccggagccaa
    10261 ctaacagagg gcggatgcct actgcctttt ctacggcatt taagatcgta gtgtttaaag
    10321 gcggaacaac agaagaaaga atggcgcctt ctatgtcaga taactggatg tggtgcattt
    10381 ccaggatatt tttaaagtga atggcgtact cggtgtctgt gcggccctgg tctgtggtca
    10441 cgcgttcgat gaaataggtc tgcttttcgt caataccacc cagtacgatg ttggtattgc
    10501 ccatatcaat ggctaaaatc atgaggggga actcctttca aatacttgaa atgtgtgtta
    10561 tgattgtatc acacaaaaat aagaaacaca accggaggaa cccttatgtt aaaaggaaaa
    10621 acaatactcc ttggagtgac cggcagcatt gccgcatata agatcgccta tctggcaagt
    10681 gccctggtaa aagcaggagc gaaagtacat gtacttatga cacagaatgc tactaatttc
    10741 attaatccca ttacctttga aacactgacg ggaaataagt gcctgatcga cacttttgac
    10801 cggaatttcc agttcagcgt ggaacatgta tctttggcaa aagaagcaga tgtttttatg
    10861 gtagcccctg cttcagccaa tgtgatcggc aaactggctc atggcattgc agatgatatg
    10921 ctgaccacta cgtttatggc ctgtacctgt ccaaagatca tctcaccggc catgaataca
    10981 cgtatgtatg aaaatcctat tactcaggat aatttaaaga ttttggagca ctacggaatg
    11041 gaagtgatcc gtcctgcaag cggatatttg gcctgcggcg atacaggagc aggcaagatg
    11101 ccggagccgg aaacattgtt tgaatatatc ctgcgcacgg taggataccc gaaagatatg
    11161 gtgggaaaga aagtcctcat cactgcggga cctaccaggg aagccattga tccggttcgc
    11221 tatatcacca atcattctac gggaaaaatg ggttatgcca ttgca
//
LOCUS       DBMDUA010000083        11255 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2905_length_11255_cov_2.826161, whole genome shotgun sequence.
ACCESSION   DBMDUA010000083 DBMDUA010000000
VERSION     DBMDUA010000083.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11255)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11255)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11255
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2905_length_11255_cov_2.826161"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /gene="trxA"
                     /locus_tag="ACZPCL_07730"
     CDS             <1..305
                     /gene="trxA"
                     /locus_tag="ACZPCL_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR1162390.1"
                     /translation="LTSETFDQAVNAGDDLVVVDFFATWCGPCKMLAPTVEKMAELHP
                     EVHFYKVDIDEDMDLATRFKVMSVPTLLYFRRGVIANKTIGVISASEMEQAIAEAK"
     gene            513..2417
                     /locus_tag="ACZPCL_07735"
     CDS             513..2417
                     /locus_tag="ACZPCL_07735"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR1162391.1"
                     /translation="MKKTPKLLILAGTVLAFLFLFGIIFYNNFIKKSIYEENSQHLLS
                     TYEQVDKTFTMFVQHNWNALSDWSNNLQYLDDAENMKTLWYDFARRKDAWQYQDFYMF
                     NENCDFITAAGRDGNAPNIQGIFLDMYETGQPVISSYIASDSNRRIVFALPMDVPYTI
                     NGITYSGLAVSYNNDVVEKLIAGNIYQGQSDCYILTSDGNVLLSLEPKTEFLQKISNL
                     FDFLNKYTNMTETEITDLKKNIKNVSTGSLQCRYNNKNYYLVYEPTGINDWTIVGIIH
                     DDVVDASMRRIQSITIVLLLILNGCIALLLTGTVFALMDSRLRMKEHEQMILLKRKEL
                     TEQMFSGLGRIVDRFAVGDLKNNRYEYHENLLKHPIYPETGYYDDLVEDISRQYLVMT
                     DTENKKMNHLLTPDYLRCVLKKKDDIFKIEYYNRSADAYLIMHVVPVEWSPEHELEKV
                     MLITQDIGQKHALENLANTDGLTGLFNERYFNSILHKKELQKLPFILFYLDLDHFKPV
                     NDTYGHDMGDKLLKEAAKRLQSCIRSNDYAFRIGGDEFSLVVCTEFDESLCCRIKEQI
                     QNRLCAPFDIDGKTLYVGSSCGYAAYPRDASTANEIRILADQRMYAEKEQHHMLSSLS
                     SSSTGRGLEK"
     assembly_gap    2449..2458
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2459..4288)
                     /locus_tag="ACZPCL_07740"
     CDS             complement(<2459..4288)
                     /locus_tag="ACZPCL_07740"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR1162392.1"
                     /translation="MMMYNADFLIAAVVILLLVLRNFLDQKRAEDLNSRVFLFFAVIG
                     ILDVIAELVSNYYITSGDGDFGLAAVVTTTIFYLFQALLPYTLLCYIMTLHDNKLISV
                     KKMLLAGIATILLVSIVLTNPFTEKLFYFDISAGYVEGPWYRLMYYSALFHLAVILIL
                     VISWKKEFGPQKIKVILDILILCGCGVVIQLLYYPLLMTGFGMSLGILALFLTINNPN
                     ANRDSLTGVYNHLYLTRRSDELIAAGKSFHIITIYLYQLKHINKVAGVEGGDYILQLT
                     AKKLEELCGSRVFRITGKRFLVLTMSLQEYEYYITQIKKMFETDMQMDADSSKPATPV
                     ILSGIVHGQKLGASGLMLEYAEYLESLSMQNGMIEVIQDDQQTMDGFLYNKKVEQYLH
                     TAIAQDLFEVYYQPVYSTEKNDFVTLEALSRLHHPELGWIAPDVFIQIAEKNHMIEQI
                     TDLQFKRICMFINEHRGLMKKLFNIKVNLSSLDLMRSDCSSHFIRMMDDMDIQHDWIQ
                     FEITETVATEYNAGLGMVVDGFMAAGVRLCLDDFGSGYANLNTVMRLPFSAIKIDRTL
                     LFDICNDKKRAMFYQSIIETFHRMGYSIVSEGVETEEEMSLLSS"
     gene            complement(4275..5834)
                     /locus_tag="ACZPCL_07745"
     CDS             complement(4275..5834)
                     /locus_tag="ACZPCL_07745"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR1162393.1"
                     /translation="MADIRSVSSGWYQIVDEKEEALDLPAMVKADQDGKIMLYNNTLT
                     PEDKNKVLSINGLQDQPEVWMGKQCLFWYEENDFLKNDQMKGKMWADVWLAEDTGKEP
                     VCLVYESNNRASLYIQAPVLGSCPAVSQKHFQESVFSFLMILGMWGTGMVSTIVFWYM
                     KAHHIVEKRFLDASLFMFICGLWCYLDSGFYQMYGEHGAVGMVLSFYAFILMSVPMLW
                     FVQNTISEKVRWVPQVWIFLLYGNAILQGILNILFHIPFVHMLFLTHIMLFFGVISMI
                     CLLWKEYKRSEAEELLFCLRAFATLGVSGVTALILYWVYSIYWYDMVFQAGIFLYTSI
                     LFWHLLQKTSRDSQFRMEQLVYEKMSMEDRMTGLKNRKAFEKYIKEIQEGKLRLENAM
                     LLFIEITGLKQINDIYGMKTGDEAVIQTARCIQKAADSDQRHKIESFRIGGTEFAVIV
                     MDPQIQPQELECLLENEVKKETENRYYISLQFGYGYLKNEDGMRNTVSDWKRQADHML
                     QRTKGAIYDDV"
     gene            complement(6129..6791)
                     /locus_tag="ACZPCL_07750"
     CDS             complement(6129..6791)
                     /locus_tag="ACZPCL_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1162394.1"
                     /translation="MGKLDYRTLRENVADEIRMKILNGDMKPGDKIIEQELASEFGIS
                     RGPVREALRQLEQEGMVEYSRNVGCSVKHIGMDDVYEIYYMRANYEMMAVRLYNAPFP
                     QETIEKMEQVLEQMKQLHKEEYRKVFELDNKFHEAILDLVSFMRLKKAWEDLNYGNIV
                     MGYNMAVDSDQVIKRQYLIHEKLLSACRTGTPAQVEQAIADHYLGSVEKLIQKVEKKH
                     EK"
     gene            complement(6865..7020)
                     /locus_tag="ACZPCL_07755"
     CDS             complement(6865..7020)
                     /locus_tag="ACZPCL_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162395.1"
                     /translation="MAGPTDALNVITPVVLTALGLVLPEIAKREQKNKFTCLTRYGKI
                     TNNKCWY"
     gene            complement(7068..7496)
                     /gene="sufU"
                     /locus_tag="ACZPCL_07760"
     CDS             complement(7068..7496)
                     /gene="sufU"
                     /locus_tag="ACZPCL_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIR1162396.1"
                     /translation="MESRNFYNEILTDHNLHPVHKEDIPDANLVLEGLNPSCGDHIWL
                     KLKVEDGIIKDGGFVGDGCAISQASADIMLDLIIGKKKEEAVKLAESFLKMIKGNATE
                     EDMDALEEAGALQDISHMPARVKCAVLGWHTMEELLKEEK"
     gene            complement(7486..8703)
                     /locus_tag="ACZPCL_07765"
     CDS             complement(7486..8703)
                     /locus_tag="ACZPCL_07765"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009446871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1162397.1"
                     /translation="MSSNPYRKDFPLLENNKVIYLDNAATSQRPEAVIQAEAQFYEKY
                     NANPLRGLYELSVEATDCYEEARETVRKFIGAGSTEEIIFTRNTTEGLNLVAYSYGFS
                     HLKAGDEVLVSIMEHHSNLLPWQMVCRQTGATLKFLECDQTGFLSDETLENAITDKTR
                     LVAIAQVSNVLGRTAPIEKIVKLARKHGAVVVMDAAQSAPHMPINVKDLEVDFMAFSG
                     HKLMGPMGIGVLYGKKELLEEMPPFLTGGEMIDSVSRTGAVFAPLPHKFEAGTVNAAG
                     AWGLKAAIEYLQQIGFDEIKRREEALTSRALEGLKEIQHVHVLGSEKAEEHCGILTFT
                     IDGVHPHDVSAILDADGIAVRAGHHCAQPLMEYLGVSSTTRASLFFYNTEEEIDAFVE
                     SMKTVRRRMGYGE"
     gene            complement(8705..9790)
                     /locus_tag="ACZPCL_07770"
     CDS             complement(8705..9790)
                     /locus_tag="ACZPCL_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufB/SufD family protein"
                     /protein_id="HIR1162398.1"
                     /translation="MSQGLDMTVNSLPSRTWNWLKMNESKLKEIEAGDTWDVKEVAGE
                     SEEIAGLTEGTAENQEVFSHIHTGMGPDMDRLGKMAGSQAIMIKSSPKSEGGSEPVRL
                     HFSCEDNKKSFQKVEILAKEGEEVNVLMDYTSPEKTSSGLAAIQTKFHIEKGAKVRLV
                     QVQLLGNEYTLLNDIGAQCEDGAQFEVLQLFLGSSKTYSGCQADLLGKESHFKADIGY
                     QGKGSQRYDMNYVAVHKGKKTVSEMNADGILDDQAFKLFRGTIDFKNGSSGSKGDEKE
                     DVLLMGDNVVNQTIPLILCAEEDVEGNHGASIGQLDEEMLFYLSSRGMSAESASKMIA
                     RARMDAVCQKIGDEQLEKLVQDYLEEE"
     gene            complement(9827..11233)
                     /gene="sufB"
                     /locus_tag="ACZPCL_07775"
     CDS             complement(9827..11233)
                     /gene="sufB"
                     /locus_tag="ACZPCL_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR1162399.1"
                     /translation="MEEKTQINDIDRSIYDIKDEEKDAYRIQAGLTPEIVEKISKEKH
                     DPAWMELFRLQSLQIYNEMKVPDWGPSIEGLDMSNIATYVRPNTKMTAKWSEVPQDIK
                     DTFERLGIPQAERKSLAGVGAQYDSELVYHNVRKEVEEQGVVYTDMESALTGPYADMV
                     RKHFMKLVKPTDHKFAALHGAVWSGGSFVYVPPHVFVEIPLQSYFRLNAPGAGQFEHT
                     LIIVDEGADLHFIEGCSAPKYNVANLHAGCVELFVGKNARLRYSTIENWSKNMYNLNT
                     KRAQVEEGGVIEWVSGSFGSHVSYLYPMSILKGRGARMEFTGITFAGKGQDLDTGAKV
                     VHAAPDTSSFMNTRSISKDGGVSTFRSSVSVTKEAKNSRSSVSCQSLMLDSISRSDTI
                     PAMDIRTKDADIGHEARIGRISDEAVFYLMSRGLSEEDARAMIVSGFADNVSKELPLE
                     YAVEMNNLIRLEMKGSIG"
BASE COUNT         3009 a         2719 c         2116 g         3401 t
ORIGIN      
        1 atttaacatc tgaaacattt gaccaggcag taaacgctgg tgatgatctg gtagtagtgg
       61 atttcttcgc tacatggtgc ggtccctgta aaatgctggc tccaactgta gaaaaaatgg
      121 cagaactcca cccggaagtt catttctata aagttgacat tgacgaagac atggatcttg
      181 caacccgttt taaggtaatg agcgtaccaa ctcttctcta tttcagaaga ggtgtgattg
      241 ccaataagac cattggcgtt atctctgctt ctgaaatgga acaggcgatt gctgaggcga
      301 agtaacaaaa ccagttttta gcagagctgt ataaaagcag ctctgttttt ttctgcgtaa
      361 aaccgctgtc actctttttc cacaatcgaa aaaaattcca gaaactccca gaatatgata
      421 tacttatatt cagatatcaa gcccaaaaac atgagtagga aacctgaaaa ccaacaagaa
      481 aagcaccaac attgaaggag gttcccgtat ttatgaagaa aacacccaaa ctgcttatac
      541 tggccggaac tgttttggca tttctttttc ttttcgggat tattttttac aacaatttta
      601 tcaaaaaaag tatctatgag gaaaattccc agcatctgct gtctacttat gagcaggtag
      661 acaaaacctt tactatgttt gtccagcaca actggaatgc cctttctgac tggagcaata
      721 atttgcaata cctggatgat gctgaaaata tgaaaactct ctggtatgac tttgccagac
      781 gcaaagatgc ctggcaatat caggattttt atatgtttaa tgagaactgt gattttataa
      841 ctgctgcagg gcgggacgga aatgccccaa atatacaggg gatctttctg gatatgtatg
      901 aaactggcca gcctgttatt tccagctata ttgcaagcga ttccaaccgc cggatcgtat
      961 ttgcgcttcc catggatgtt ccatatacca taaatggcat aacctattcc ggacttgctg
     1021 tcagctataa taatgatgta gtggaaaaac tgatcgcagg taatatttat cagggacaaa
     1081 gtgactgcta catccttaca tccgatggca atgtcctcct ttcccttgaa cccaaaacag
     1141 aatttctcca gaaaatatct aatctctttg attttttaaa caagtatacg aatatgacag
     1201 aaactgagat aactgacctg aaaaagaata ttaagaatgt ttctaccgga agtctccagt
     1261 gccgttacaa caacaaaaac tattatctgg tatacgaacc caccgggatc aatgactgga
     1321 ccattgtggg tattatccat gatgatgttg tagatgccag tatgcgccgt atacagtcca
     1381 tcaccattgt cctgctcctg atcctgaacg gatgtatcgc cctgcttctt acgggtactg
     1441 tttttgccct gatggattcc aggctgcgca tgaaagaaca tgagcaaatg atcctgctaa
     1501 agcgcaaaga gcttactgaa cagatgtttt caggacttgg aaggatcgtt gaccgttttg
     1561 ctgtaggcga tctgaaaaac aaccgctatg aatatcatga aaacctgctg aagcacccca
     1621 tctatccgga aaccggatac tatgatgatc tggttgagga tatttctcgc cagtatctgg
     1681 tgatgactga tacggaaaat aaaaagatga accatctcct tacaccagat tatctgcgct
     1741 gcgtcctgaa aaaaaaggat gatatcttta aaattgaata ctacaaccgt tccgccgatg
     1801 catacctgat catgcatgtt gtccccgttg aatggagtcc tgaacatgaa ctggagaagg
     1861 ttatgcttat cacccaggat attggacaga agcatgcctt agagaacctt gccaatacag
     1921 acggccttac tggtttattt aacgagcgtt attttaactc catactgcat aaaaaggagc
     1981 ttcaaaagct ccctttcata cttttctatc tggatctgga ccattttaaa cctgttaatg
     2041 acacctatgg tcatgacatg ggtgataagc ttttaaaaga agctgccaaa cgccttcagt
     2101 cctgtatccg cagcaatgac tatgctttcc gcatcggcgg tgatgaattc agcctggtag
     2161 tgtgtactga atttgatgaa agtctctgct gcaggatcaa ggaacagata caaaaccggc
     2221 tctgcgctcc ttttgatatt gacggaaaaa ccctgtatgt tggttccagc tgcggatacg
     2281 ccgcctatcc cagggatgca tctactgcta atgagatccg tatccttgca gaccagcgta
     2341 tgtatgcaga aaaggaacag catcacatgc tcagcagttt aagcagttcc tccaccggaa
     2401 gaggtctgga aaaataatat ccctggatca tatccacacc ccaccttcnn nnnnnnnngc
     2461 tgcttaacag actcatttcc tcttctgttt ctactccttc tgaaactatg ctgtaaccca
     2521 tccggtggaa ggtttcaata atgctctgat agaacatggc acgtttttta tcattacaaa
     2581 tatcaaaaag aagtgttctg tctatcttga tagcagaaaa tggcagtctc atgactgtat
     2641 tcaggtttgc atatccggaa ccaaaatcgt ccagacacag tctgaccccg gcagccataa
     2701 acccatctac taccattcct aatcctgcgt tgtattctgt agcaaccgtt tctgtgatct
     2761 caaactgtat ccagtcatgt tggatatcca tatcatccat catacggata aaatggctgc
     2821 tgcagtcact gcgcatcaga tccagggaag acaaatttac ttttatatta aagagcttct
     2881 tcatcagacc ccggtgttca tttataaaca tgcagatgcg tttaaactgc agatctgtga
     2941 tctgttcgat catatgattt ttttctgcga tctgtataaa gacatccggc gcgatccagc
     3001 ctaactccgg atgatgcaga cggcttaatg cttccagtgt aacaaaatca tttttctctg
     3061 tagaataaac cggctgataa tatacttcaa aaagatcctg ggcaatagcc gtgtgcagat
     3121 actgctctac ttttttatta tataaaaagc catccatggt ctgctgatca tcctgtatca
     3181 cctctatcat gccattttgc atagacaatg attccagata ctctgcatat tccagcatca
     3241 gcccgctggc accaagtttt tgtccatgga cgatcccact gaggatcaca ggcgttgcag
     3301 gtttactgct gtctgcatcc atctgcatat ctgtttcaaa catctttttt atctgggtaa
     3361 tataatactc atactcctgc aatgacatag tcagaactaa aaaacgtttt cccgtaatac
     3421 ggaaaacccg gcttccacac agttcttcca gtttttttgc tgtaagctgc agaatataat
     3481 caccgccttc aactccggca actttattga tatgctttag ctggtaaagg tatattgtga
     3541 ttatatggaa ggattttcct gctgcgatca gttcatcgct ccttctggta aggtaaaggt
     3601 gattatatac tcctgtaagg ctgtcccggt tcgcatttgg attattgata gtaagaaaca
     3661 gagccagtat tccaagactc atgccaaaac ctgtcatcag cagagggtaa tacaaaagct
     3721 ggatcactac cccacatccg cacaggatca ggatatccag gatcaccttt attttctgag
     3781 ggccaaattc ttttttccag gagataacaa ggatcaaaat cactgccaga tggaaaaggg
     3841 cactgtaata catcagtctg taccatggtc cttccacata tccggctgat atatcaaaat
     3901 aaaacaattt ttcagtaaag ggattggtaa gcacaatgct gaccagtaaa atagttgcaa
     3961 taccagccag cagcatcttc ttcactgaaa taagcttatt atcgtgcaat gtcataatat
     4021 aacaaagaag tgtatatgga agcaatgcct gaaacagata aaaaatagtt gtggtcacca
     4081 ctgcagccag gccaaagtcc ccatctcctg aagttatata ataattgctt actaattcag
     4141 cgatcacatc caggatccca ataactgcaa agaaaagaaa taccctgcta tttaaatcct
     4201 ctgccctttt ctgatccagg aaatttctca aaaccaacag caatatgacc actgctgcga
     4261 tcagaaaatc tgcattatac atcatcataa atagccccct ttgttctttg caacatgtga
     4321 tctgcctgac gtttccagtc acttactgta tttcgcatcc cgtcctcatt tttcagataa
     4381 ccatatccga attgtaaaga aatataatac ctgttttccg tttccttttt cacttcattt
     4441 tcaaggaggc attccagctc ctgcggctgt atctggggat ccattacgat cactgcaaac
     4501 tctgtaccgc caatacggaa actttctatc ttatgcctct ggtcactatc tgctgccttt
     4561 tggatacacc gggctgtctg gatcactgct tcatctcctg ttttcattcc gtaaatatcg
     4621 ttgatctgtt tcagtcctgt tatctctata aataaaagca tcgcgttctc aagccggagc
     4681 tttccttcct ggatctcttt tatgtatttt tcaaatgctt tcctgttttt taatccagtc
     4741 atcctgtctt ccattgacat tttttcatag accagctgct ccatcctgaa ctgactgtcc
     4801 ctggatgttt tttgcagtaa atgccagaac aggatcgatg tgtataaaaa gattcctgcc
     4861 tgaaagacca tatcatacca gtagatcgaa tatacccagt acaggataag tgctgtcaca
     4921 ccgctgactc ccaatgtggc aaatgcccgc agacaaaaca gaagttcttc cgcttcactt
     4981 cttttgtatt ctttccacag aagacagatc atggatataa ccccgaaaaa cagcataatg
     5041 tgtgttaaaa acagcatatg cacaaatggg atatgaaaca ggatattgag tattccctgt
     5101 aaaatggcat ttccataaag aaggaagatc catacctgcg ggacccaccg tactttctca
     5161 cttattgtat tctgcacaaa ccagagcatt ggaactgaca taagaataaa tgcataaaaa
     5221 gaaagaacca tcccaacagc gccatgctct ccgtacatct gataaaaacc tgagtccagg
     5281 taacaccaca agccacagat gaacataaac aatgatgcat ccagaaaacg cttttcaaca
     5341 atatgatggg ctttcatata ccagaaaaca atagtagaaa ccattcccgt tccccacatt
     5401 ccaaggatca tgagaaatga aaatacagat tcctggaaat gcttttgtga aactgccgga
     5461 cagcttccta aaacaggtgc ctgtatataa agggaggctc tgttattgct ttcataaaca
     5521 aggcagaccg gctcttttcc tgtatcttct gccagccaca catctgccca catttttccc
     5581 ttcatctggt cattctttaa aaaatcgttt tcttcatacc agaacagaca ctgttttccc
     5641 atccacactt ccggctgatc ctgaagaccg tttattgaca ggaccttatt tttatcttcc
     5701 ggagtcagtg tattattata aagcatgatc tttccatcct gatctgcctt aaccatggca
     5761 ggaagatcaa gggcctcttc tttttcatct actatctgat accaaccact gctgacagaa
     5821 cggatatctg ccatttctcc atctgcccct gaattccata acatatgtat caaaaccatc
     5881 aggataaaaa atatacagat caaaccggcc cattctgttt ttttccatac attgcttttt
     5941 tcatgatcac tattttcctc gcccaaacag acaattcatc cccccctact taaaaaatta
     6001 taacagaatc tatattaatt gtcaccattt gcagtgctgg attgcatttt tctatcaacg
     6061 taaaaagcag taaaaccata ttgttctttc cggcttcact gcttttttaa tgcgttatat
     6121 gtctttattt acttttcgtg ctttttttcc actttctgta tcagcttctc cacgctccca
     6181 agataatgat ctgcaatagc ctgttccacc tgggcgggag tacctgttct acaggcactt
     6241 aacagttttt cgtggatgag atactgcctt ttgataacct gatcactgtc cactgccata
     6301 ttatatccca ttacaatatt tccgtagttt aagtcttccc aggctttttt aaggcgcata
     6361 aagcttacaa gatctaggat tgcttcatgg aacttattat ccagttcaaa taccttgcgg
     6421 tattcttctt tatgaagctg cttcatctgc tctaaaacct gttccatttt ctcaatggtt
     6481 tcctggggaa aaggagcatt atacagtctt actgccatca tttcatagtt ggcacgcatg
     6541 tagtatatct cataaacatc atccatgcct atatgcttta cagagcagcc tacatttctg
     6601 gaatattcca ccatgccttc ctgctccaac tggcgcagtg cttcccgcac cgggccacgg
     6661 ctgatgccaa attccgatgc cagttcctgc tctatgatct tatctcccgg cttcatatca
     6721 ccgtttaaga tcttcatcct tatctcgtct gctacatttt cccttaaagt ccgataatca
     6781 agctttccca taatactctg taagtcccta aaaaatccct ggtacattgt ttacgaaaca
     6841 atgtactgat cactgtcata aacattaata ccaacatttg ttgttggtta ttttaccata
     6901 acgcgttaaa caagtaaact tatttttctg ctctctcttt gcgatctcag gaagtaccag
     6961 tcccagagcg gtcagtacaa caggtgtgat cacatttaac gcatctgtcg ggcccgccat
     7021 atactggcga gcccgtattt aataggatct gtaaatatac attctatcta tttttcctct
     7081 tttaacagtt cttccatggt atgccatccc aaaacagcac attttactct ggccggcata
     7141 tgggaaatat cctgtaatgc ccctgcttct tccagggcat ccatatcttc ctctgttgcg
     7201 tttcccttga tcatctttaa gaaactttct gccagtttca cggcttcctc tttcttttta
     7261 ccgatgatca gatccaacat gatatctgca gatgcctggg aaatggcgca gccatcacct
     7321 acgaaaccac cgtctttgat gatgccgtct tctactttta actttaacca gatgtggtca
     7381 ccgcagctgg gatttaagcc ttccagtacc agattggcat ccgggatatc ttctttgtgg
     7441 accggatgca gattgtggtc cgttaatatt tcattgtaaa aatttctact ctccataacc
     7501 cattctcctt cttactgtct tcatgctttc tacaaaagca tcgatctctt cttctgtatt
     7561 gtagaagaaa aggctggctc ttgtggtaga agaaacgccc agatactcca tcagaggctg
     7621 tgcgcagtga tgtccggcac gaacggcaat tccgtctgcg tctaaaatgg cgcttacatc
     7681 atggggatgt acgccgtcta tggtaaatgt aaggataccg cagtgttcct ctgctttttc
     7741 agaacctaac acatgaacat gttggatctc tttaaggcct tctaaggctc tggaagtcag
     7801 cgcctcttcc cggcgcttta tctcatcaaa gccaatctgc tgcagatatt caatggctgc
     7861 tttcagtccc catgcgcccg ctgcattaac ggtacctgcc tcaaatttgt gaggaagtgg
     7921 agcaaataca gcacctgtac ggctgacgga gtcgatcatc tctccgcctg taaggaatgg
     7981 aggcatttct tccaacagct ctttctttcc atagagaaca ccgatgccca taggtcccat
     8041 taatttatga ccggaaaatg ccataaaatc cacctccagg tcttttacat taattggcat
     8101 atgtggcgca ctctgtgctg catccatgac aactacggca ccatgttttc ttgccagttt
     8161 tacgatcttt tcaatgggag cagtccgtcc cagtacattg gatacctggg caatggctac
     8221 caggcgggtc ttatctgtaa tggcattttc cagtgtttca tcacttaaaa atccggtctg
     8281 gtcacattcc agaaatttaa gcgttgcacc ggtctgacgg cataccatct gccatggaag
     8341 gagattgctg tgatgctcca taatgcttac cagcacttca tctcctgctt ttaaatggct
     8401 gaaaccatag ctgtaagcca ccagatttaa gccttcggta gtatttctgg tgaaaatgat
     8461 ctcttctgtg cttcctgcgc caatgaactt gcggaccgtt tctcttgctt cttcgtagca
     8521 gtctgtggct tctacactaa gctcataaag gcctcttaat ggatttgcgt tgtatttttc
     8581 ataaaactgt gcctctgcct ggatcacagc ctccggcctc tgggaggtgg ctgcattgtc
     8641 cagatagatc accttattat tttccagaag aggaaaatct tttctgtatg ggttgctgct
     8701 catgttattc ctcctctaaa tagtcctgta ccagcttttc taactgctca tcgccgatct
     8761 tctggcagac tgcatccatc cttgctctgg cgatcatttt gctggcgctt tctgcgctca
     8821 tacctctgga agacagatag aacagcattt cttcatctaa ctgtccaata gaagcaccgt
     8881 ggtttccttc tacatcttcc tctgcacaaa ggatcagagg aatggtctgg ttgactacat
     8941 tgtcccccat aaggagtacg tcttcctttt cgtctccctt ggagccggaa gaaccatttt
     9001 tgaaatcaat ggttccacgg aacagcttaa atgcctgatc atccagaatg ccgtctgcat
     9061 tcatttcact gacggttttt ttgcccttat ggactgccac atagttcata tcatagcgct
     9121 ggctgccttt cccctgataa ccgatatctg ctttaaaatg gctttctttt cctaaaagat
     9181 ctgcctgaca gccggaatag gttttgctgc ttcctaaaaa cagctgtaac acttcaaact
     9241 gggccccatc ttcacactgg gcgccaatat catttaataa tgtatactca tttcctaaca
     9301 gctgcacctg taccagacgg acttttgccc ctttttcaat gtgaaatttt gtctggatgg
     9361 ctgccagacc ggaagatgtt ttttccgggg aagtatagtc cataaggacg tttacttctt
     9421 ctccctcttt tgccaggatt tccactttct ggaaactctt tttattatct tcacaggaaa
     9481 aatgaagacg gaccggttca gatcctccct cagactttgg actgcttttt atcatgattg
     9541 cctgtgatcc ggccattttt cctaaacggt ccatatccgg acccattcct gtgtggatat
     9601 gggagaaaac ttcctgattt tcagctgtgc cctctgtcag accagctatt tcttcggact
     9661 ctccggctac ttcttttaca tcccaggtat ctcctgcttc tatctctttt aacttactct
     9721 cattcatttt cagccagttc catgttctgg aaggaaggct gtttactgtc atatcaagtc
     9781 cctgactcat atgggacctc ctttccttta accagtaccc ctttgcttaa ccaatgcttc
     9841 ccttcatctc cagacggatc aggttgttca tctccactgc atactccaga ggaagttcct
     9901 tggaaacatt gtctgcaaat ccgctgacga tcatggcacg ggcatcttct tcgcttaagc
     9961 ctctggacat aagataaaag actgcttcat cgctgatacg tccgatccgg gcctcatggc
    10021 cgatatctgc atctttggta cggatatcca tagccgggat tgtatcggat ctggaaatgg
    10081 agtctaacat tagagactgg caggaaacgg aggatctgct gttttttgcc tcttttgtta
    10141 cagaaacaga gcttctgaag gtgctgacac caccgtcctt ggaaatggaa cgggtattca
    10201 taaaggatga tgtatcaggt gctgcatgga ctacctttgc gcctgtatcc aggtcctggc
    10261 cttttcctgc aaaggtaatc cctgtaaatt ccattcttgc gccacggccc tttaagatgc
    10321 tcattgggta cagataggaa acatgggaac cgaaggagcc ggatacccat tcaataacgc
    10381 cgccttcttc tacctgtgca cgcttggtat tcaggttata catgttcttg gaccagtttt
    10441 caatggtgga ataacgcagt cttgcatttt ttccaacaaa cagctccacg cagcctgcat
    10501 gaaggttagc cacgttgtat ttcggtgcgg aacatccttc gataaagtga aggtctgcgc
    10561 cttcatctac gatgatcagt gtatgttcaa actgtccggc gcctggtgca tttaaacgga
    10621 aataggactg caatggtatc tcaacaaata catgaggcgg tacatataca aaggatccgc
    10681 cggaccatac agctccgtga agagcagcaa atttatggtc tgttggcttt accagcttca
    10741 tgaaatgttt tcttaccatg tctgcataag gaccggtcaa ggcgctttcc atgtcagtgt
    10801 atacaacgcc ctgctcttcc acttctttac gaacattgtg gtaaaccagc tcggagtcat
    10861 actgggcgcc tacaccagcc agtgatttcc gctctgcctg aggaattccc agacgttcaa
    10921 aggtatcctt aatatcctgc gggacctcag accattttgc agtcattttt gtatttggac
    10981 ggacataggt ggcgatattg gacatatcca gtccttcaat agaagggccc cagtccggta
    11041 ctttcatttc attgtagatc tgaagagatt gcagacggaa cagctccatc catgccggat
    11101 catgtttttc cttggaaatt ttctctacga tctccggagt cagacctgcc tggatcctat
    11161 aggcgtcttt ttcttcatct tttatgtcat aaatacttct gtctatatca ttgatctgtg
    11221 ttttttcttc catatgcttt cgatttcctt tccgg
//
LOCUS       DBMDUA010000084        11223 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2911_length_11223_cov_3.578133, whole genome shotgun sequence.
ACCESSION   DBMDUA010000084 DBMDUA010000000
VERSION     DBMDUA010000084.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11223)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11223)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11223
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2911_length_11223_cov_3.578133"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="ACZPCL_07780"
     CDS             complement(<1..510)
                     /locus_tag="ACZPCL_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HIR1162400.1"
                     /translation="MINANKKRLRRTMMFLNAQKPGLIKDPYIYKPDSIMLDLEDAVA
                     ENQKDAARFSLYHALKEINYRGCERVVRINGLDTPYWEEDIRCAVAGGCDAIRIPKTE
                     SALDVQRVEAVVEECEKKYGIPEGHTLIMAAIESARGVMKALDICEASERLFGIALSG
                     GDYTKDLQTH"
     gene            complement(507..806)
                     /gene="citD"
                     /locus_tag="ACZPCL_07785"
     CDS             complement(507..806)
                     /gene="citD"
                     /locus_tag="ACZPCL_07785"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HIR1162401.1"
                     /translation="MEIKKPAMAGTLESSDCQVTVEPGNGSVEFSLESAVINQYGNQI
                     RKVVLQTLENLDVKDVKISVVDKGALDCTIKARVEGAVFRSVDQIEDIPWGGAIR"
     gene            complement(989..3295)
                     /locus_tag="ACZPCL_07790"
     CDS             complement(989..3295)
                     /locus_tag="ACZPCL_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HIR1162402.1"
                     /translation="MVKLYDEGIYLVNGTEIVPESEAAKVQALTGKAANKEEAKKGTM
                     ACSILSAHNTSGDMEHLKLKFDAMASHDITFVGIVQTAKASGMEKFPLPYVLTNCHNS
                     LCAVGGTINEDDHMFGLSAAKKYGGIYVPPHIAVIHQYMREMHAGCGRMILGSDSHTR
                     YGALGTMAIGEGGGELVKQLLCDTYDVAYPGVVAIYLTGKPGPGIGPQDVALSIIGAV
                     FKKGYVKNKVMEFVGPGVASMTTDYRNGVDVMTTETTCLTSIWRTDEDTKAFLTAHGR
                     GDAYKELNPADVAYYDGCVYVDLSTIKPMIALPFHPSNTYEIDELNANLGDILRSVEK
                     EAEKVSGGRAPFTLTDKIENGRLRVQQGIIAGCAGGNFTNVVEAAHALKGKSCGDGEF
                     SLAVYPSSQPVFIDLVRKGLIADLMEAGAIIRTAFCGPCFGAGDTPCNNGLSIRHTTR
                     NFPNREGSKPGNGQMSAVALMDARSIAATAANGGILTPATDLDCWGDVPEYNFDKTVY
                     DRRVYNGYRAPHEEDSLVYGPNIKDWPEMSPLTDNILLKVCSKIMDEVTTTDELIPSG
                     ETSSYRSNPLGLAEFTLSRRDPEYVGKSKAVDKLEKARTAGQKPSELDADLNGVFDAI
                     HTISGQENVNEMETEIGSMIYAVKPGDGSAREQAASCQRVIGGLANITKEYATKRYRS
                     NVMNWGMIPFQMVEEPEFEVGDYIYVPGAKAALDSDLKDIKAYVINADTKAVKEISLY
                     VADMTADERKIVKAGCLINYNRARKGLK"
     gene            3564..4502
                     /locus_tag="ACZPCL_07795"
     CDS             3564..4502
                     /locus_tag="ACZPCL_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1162403.1"
                     /translation="MTSRELEYIKTVADEKSISSAARKLYIAQPSLSQSLQRIEENLG
                     AKLFNRTPSGLTLTYAGERCYQMACQVLKIYSDFEAEISDINQLKTGRIVMGTTNHLG
                     TVILPEILLEFKENSPSVELEILEDNTGNLEAKLLTGAMDFALLHAPKLAPNPLLNYE
                     FLSEDPFVIALAAGHPLIEKAQKKPGYLFPVLDVRLLKNEPLILLHPQQRIRHVSDAI
                     LAKAKITPNIVLTIKNYETAQRLAGQGIGITFLPMDYAALTYADHPPVLLSIDESYSP
                     SWDLSIATLKGGFLSRADQYFLELVRQTFAQEPNDK"
     gene            complement(4579..6429)
                     /gene="asnB"
                     /locus_tag="ACZPCL_07800"
     CDS             complement(4579..6429)
                     /gene="asnB"
                     /locus_tag="ACZPCL_07800"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR1162404.1"
                     /translation="MCGIAGFYQSHFDYLTEEDHYRQILEDMEKVQKHRGPDEQGNYL
                     DSHFGLAHVRLSVRDLVSGRQPILKKTGDKTFGIVYNGELYNTTELKEDLLKKGWEFS
                     TTGDTEVVLTGYMEYGPDFVKQMNGIFAFGIMDPVKDCLVLFRDRLGVKPLFYTCFHD
                     QVIFSSEIKGLFAYPGVTPKLDKKGLNEIFSIGPAKTYGCGVFKDIEELLPAHYLVCN
                     AAGLHTECYWKLESKPHTDSYEQTVEKTAFLVRDAICRQIISDVPVCTFLSGGVDSSL
                     VSAVCSAELKKKNKQLTTFSFDFKDNHKYFTANAFQPSMDRPFVDIMVKYLGSDHHYL
                     ECDNVTQADLLYTSVEARDLPAMADVDSSLLYFCSQVSRTHKVALTGECADEIFGGYP
                     WFHKKECFEAKTFPWTMDLSARKVLLSDDFIRELHMDEYVQATYEKSVAETPRLAEDT
                     PEEARRREIAWLNLRWFMQTLLDRMDRTSMHSGLEARVPFADHRIVEYLWNVPWYMKT
                     RDGVAKHLLRESGKGLLPDEVLWRRKSPYPKTYDRAYEALLVGRVREILEDNTSPVLQ
                     FLDKAKTEKFLESPSDYGKPWYGQLMAGPQMLAYMIQVDYWLKKYNIEIV"
     gene            complement(6500..7936)
                     /locus_tag="ACZPCL_07805"
     CDS             complement(6500..7936)
                     /locus_tag="ACZPCL_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HIR1162405.1"
                     /translation="MPEAYIALTPVHWFYLVGVLVILFTMILRKDTPLVCIAFLFGIG
                     VVGKCSLAGGIQTIFSSIIYAISEFREVIATIALVTAFSKCLRELGSDALLMKPVSKV
                     MRTPAVSWWILGGIMFVFSLFLWPSPAVALVGAIVLPIAVQSGLTPLAAAMAMNLFGH
                     GFALSYDAVIQGAPAISAGAADISTTDILSKGRPLFWIMGITCVCSAFLLNRMTLAGQ
                     RKNEDQRNDLKITDLPEKQEDSGEETPEEKGDRKKQYSTTAKALAVLTPIAFLMDILF
                     MFLFKLKGGDATSLVAGTAVILMCVGAVMEFKAGSLEKVTEYVTDGFLFAIRIFAPVI
                     VIGAFFFLGGNGITHILGDSYERGILNDWALWLAHHAPLNRYMTALLQLVIGGLTGLD
                     GSGFSGLPLTGALARTFGTATGASIPVLACLGQISAIFIGGGTVVPWGLIPVAAICNV
                     DPVELARKNMLPVFIGLTCTFLVACLVL"
     gene            complement(8098..9240)
                     /locus_tag="ACZPCL_07810"
     CDS             complement(8098..9240)
                     /locus_tag="ACZPCL_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HIR1162406.1"
                     /translation="MRKFKTVFMRGGTSKGCMFHKEDLPEDRKEWDNIFLQCMGDPDP
                     KQIDGLGGTVSSNNKIVIVWKSKEPGVDVEYLVGQVIVGKSQVDYKSNCGNMTAAVGP
                     YAVEEGMVDIVEPITTVRMLNRNTDKYINVTVPIDPETKTFAQEGDCAIAGVDGTAAE
                     LKVNFLNPAGAKTGKLLPTGNPKDVLDIPGFGPIEATILDVSNPMVLVRAEDIGLTGR
                     ELPEEVNSNKAASEILEKIRGVACCKMGFAKDLEDATSNSPAVPKVGFFTKPQSYVDI
                     AGQQVNAEDMDVCARVISVFKCHKACPLTSASSISVAAFLKGSLLYEELGKPEEGKTN
                     VRIGHPSGVMTMYPDLKEENGELVLPGVAVQRTARRIMDGMVYVRK"
     gene            complement(9252..10409)
                     /locus_tag="ACZPCL_07815"
     CDS             complement(9252..10409)
                     /locus_tag="ACZPCL_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR1162407.1"
                     /translation="MSAISQAQEKFGELIQSEFERIERMKQDTEVKDFSKLDKIVVGI
                     LPGDGIGPIIMEQAVRVIKALIPDEIASGKVELRHIEGMTIENRAAKLQSLPDDVFEE
                     IKKCDVIIKGPMVTPRAGEPWPNLVSANSLLRRGLELFAAVRPIRIPDKGIDWTFFRE
                     NIEGEYIWGNKGIQVDEDLAVDFKVQTKQGSERIARAAFEFARKNGKKNVTIVTKANI
                     VKLADGNFIKAVRKVGEEYPEIEIQERLVDAMCAKMLDPEFNKGIEVVVLPNLYGDIV
                     TDIAAEHQGGLGTASSSNIGNKYAMFEAIHGTAPYLMSHGRGEYADPSSLIRAAGMML
                     AHIGYADKKVLLEKALDVCTTEKKVVLTTFTEDASAKEYTDYIIETIEKLK"
     gene            complement(10598..>11223)
                     /locus_tag="ACZPCL_07820"
     CDS             complement(10598..>11223)
                     /locus_tag="ACZPCL_07820"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HIR1162408.1"
                     /translation="GIPMNLGACILPVVVPVFRRRYPGVKVFIRENNSQELEKMMLSH
                     KVDFCIMHFQKALDEVCYDEFSEDPFYLAVPEIFQSQVSLSEEKSLKAEDIRQLKKIP
                     FVMVASRQKLRQVADEILRKAGIRPDICCTTKSMETAKRLAAGGVGATFLPKSYMTLY
                     SGTEGLSCYPLDKELGASWKLVAAYPADEKLSRASREFLRTLKECLE"
BASE COUNT         2819 a         2935 c         2236 g         3233 t
ORIGIN      
        1 gtgtgtctgt aagtcctttg tatagtcacc accggaaaga gcgataccaa acagtctctc
       61 agatgcttcg cagatatcca gggctttcat aacacctctt gcagactcga tcgctgccat
      121 gatcagtgtg tggccttccg ggataccgta cttcttttcg cactcttcta ctacagcttc
      181 tactctctgt acatccagtg cgctttctgt cttagggata cggatggcat cacagccgcc
      241 tgctactgca cagcggatat cttcttccca atatggggtg tcaaggccat tgatacggac
      301 aactctctca caacctctgt agttgatctc ttttaatgcg tgatacagag agaaacgtgc
      361 tgcatctttc tggttctcag caactgcatc ctccagatcc agcataatgg agtctggttt
      421 gtagatatat gggtctttga tcagacctgg cttctgtgca tttaagaaca tcatggttct
      481 tcttaaacgt ttcttattcg cattgatcat cttattgcac ctccccacgg aatgtcctcg
      541 atctggtcta cagaacggaa taccgcacct tctactcttg ccttaatggt gcagtctaaa
      601 gcgcccttat ccaccactga gatttttaca tcctttacat ccaggttctc cagtgtctga
      661 aggactacct tgcggatctg gttgccatac tgattgatga cagcactttc caggctaaat
      721 tccacagaac catttcccgg ttctacggtt acctgacagt cactggattc cagagttcct
      781 gccatggccg gtttcttgat ctccatcttt atttcctcct atttttaacg atgtcttttc
      841 aacatctttt tgttaccccc gcatctgtgc aaaagcattt tcaaacgggc actgccacac
      901 attttccaga tcattttgaa tcaggaaaat atgtggcact gtgtgtttat actcaggtct
      961 cccgcgctga cgcgctcatt acattatatc actttaagcc ttttcttgca cggttataat
     1021 tgatcagaca gcctgctttt acgatctttc tttcatctgc agtcatatct gccacgtaca
     1081 gactgatctc ttttaccgcc ttggtatctg cattgatcac ataagcctta atgtctttta
     1141 aatcactatc cagtgctgct tttgcacctg gaacgtagat atagtctcct acttcaaact
     1201 caggctcttc taccatctgg aatgggatca ttccccagtt cattacatta gaacgataac
     1261 gcttggttgc gtattctttt gtgatgtttg caagtccgcc gatgactctc tggcagcttg
     1321 cagcctgttc tcttgcagaa ccatcaccag gctttacagc atagatcatg cttccgatct
     1381 ctgtttccat ttcatttaca ttttcctgac cggaaatggt atggatggca tcaaataccc
     1441 cattcaggtc tgcatctaac tctgatggct tctgtcctgc tgttcttgcc ttctctaatt
     1501 tgtctaccgc cttgctcttt cctacgtatt caggatctct tctggataat gtaaactcag
     1561 ccagtcctaa tgggttggaa cgataggaag aggtctcacc agatgggatc agctcatcgg
     1621 tcgtggttac ttcgtccatg atcttggagc atacctttaa gaggatattg tcagttaatg
     1681 ggctcatttc cggccagtcc ttgatatttg gaccatatac caggctgtcc tcctcatgag
     1741 gtgctctgta accattgtat acacggcggt cataaacagt cttatcaaag ttgtattcag
     1801 gaacatcgcc ccagcagtct aagtcggtag ctggtgtaag gataccgccg tttgctgcag
     1861 ttgctgcgat agaacgtgca tccattaatg ctactgctga catctggccg tttcctggct
     1921 tgctgccttc ccggttcggg aagtttctgg ttgtatgacg gatacttaag ccattgttgc
     1981 aaggtgtatc accggcgccg aagcatggtc cgcagaatgc ggttctgatg atagcgcctg
     2041 cttccatcag atctgcgatc aggccctttc ttaccaggtc gataaataca ggctgggaag
     2101 aaggatatac agccagtgag aattcgccat cgccgcagct ctttcccttt aatgcatgtg
     2161 ctgcttctac tacgttggtg aaattaccgc ctgcacagcc agcgataatt ccctgctgta
     2221 ctcttaaacg tccgttttcg atcttatcag tcagtgtaaa tggagctctt ccgccgctga
     2281 ctttctcagc ttctttctct acgcttctta agatatcgcc caggtttgca tttaactcat
     2341 caatctcata tgtattgctt ggatggaacg gaagggcgat cattggcttg atggtgctta
     2401 aatctacgta aacacagcca tcatagtaag ctacatctgc cggatttaat tccttgtaag
     2461 catctcctct gccatgagca gttaaaaatg cctttgtatc ttcatcagtg cgccagatag
     2521 atgttaagca ggtagtttct gttgtcataa catctacgcc gtttctgtag tcggttgtca
     2581 tagaagcaac accaggtcct acaaattcca ttactttatt ctttacataa cccttcttga
     2641 atactgcgcc gataatagaa agtgccacgt cctgaggtcc gatgccaggt cccggctttc
     2701 cggtaaggta aattgcaaca acacctggat aagcaacatc atatgtatca cataacagct
     2761 gttttaccag ctcgccgccg ccttcaccga tagccatggt tcctaatgca ccgtagcgag
     2821 tgtgggagtc agaacctaag atcatgcgtc cgcagcctgc atgcatttca cgcatgtact
     2881 gatggataac tgcaatgtga ggtggaacat agatgccgcc atacttcttt gctgcggaaa
     2941 gaccaaacat gtggtcatct tcgttgatgg ttccgcctac tgcacaaagg gagttgtggc
     3001 agttggtcag tacatatggc agtgggaatt tttccattcc ggatgccttt gctgtctgga
     3061 caataccaac gaatgtgata tcatgggatg ccattgcatc gaatttaagt tttaagtgct
     3121 ccatatctcc tgaagtgttg tgggcactta aaatggaaca ggccattgtg cctttctttg
     3181 cttcttcttt atttgctgct tttcctgtaa gagcctgtac ttttgctgcc tcagactccg
     3241 gtacgatctc ggtgccgttt acaaggtaga tgccctcatc atataacttt accataatgt
     3301 ttcttctcct tttctgttct tgaatggtta ttgaccatcc tctcccggag gagctggtct
     3361 tttattatct gcttcttaag tttgtttctt tttgtttctg aattaactat acacctcttt
     3421 acttttatag gcaaatacta tatatataat aacagatata catttttacc tatgcgatag
     3481 cgaacctcct gcgtcagcag gcaaataagc gagcaaacgc gcgttcgcga gtataacacc
     3541 aagtacatac aaggaggctc cccatgacca gccgtgaatt agaatacata aaaacagtag
     3601 cagatgaaaa aagcatctcc tctgctgcca gaaaacttta catcgcccag ccgtccctaa
     3661 gccagtcttt acagaggatc gaggaaaatc tgggggcaaa actttttaac aggacgcctt
     3721 ccggcctgac actgacctat gcaggagagc gctgctatca gatggcctgc caggttctta
     3781 agatctacag tgattttgaa gcagagatca gcgacatcaa ccagttaaaa accggacgta
     3841 tcgtaatggg gactaccaac catttaggta ctgtcatcct ccctgaaata ctgctggaat
     3901 ttaaggaaaa cagcccttct gttgaactgg agatcctgga ggacaatacg ggaaatctgg
     3961 aagcaaaact tcttaccggc gctatggatt ttgctcttct ccatgccccg aagctggctc
     4021 caaatccttt attaaattat gaatttctct cagaggatcc ttttgtcata gcacttgctg
     4081 ccggacatcc cctgatcgaa aaggcgcaaa aaaaaccggg atatctcttc ccggttttag
     4141 atgtacgttt attgaaaaat gaacctttga tcctgctcca tccccagcag cgcatccgtc
     4201 atgtatcgga cgccatactg gcaaaagcaa agattacgcc aaatatcgtg ctgacgataa
     4261 agaattacga gacagcccag cgtctggccg gtcagggcat tggcattacc tttctgccta
     4321 tggattacgc cgccctgacc tatgcagacc atccgccggt gttattatcc attgatgagt
     4381 cctacagccc cagctgggat ctgagtattg ccaccttaaa gggcggattt ttgtccagag
     4441 cggatcagta tttcctggaa ctggtacggc agacatttgc ccaggaacct aatgacaaat
     4501 agtacatccg gaatttttct cctctcattc ctgtacctgc caatatgcaa cgctcccgcc
     4561 cacaagggcg ggatgctatc acacgatctc aatattatac ttcttcaacc aataatccac
     4621 ctgtatcata taagccagca tctgcggccc cgccatcagc tgaccatacc atggctttcc
     4681 ataatctgag gggctttcca ggaatttctc tgttttggcc ttatccagaa actgcaatac
     4741 aggtgaggta ttgtcttcta aaatctcacg cacccggccc accagcagcg cctcataagc
     4801 tctgtcatag gtctttggat acggggattt tctgcgccac agcacttcat ccggcagcag
     4861 accttttcca gactctctta gcagatgttt tgccacccca tcccttgttt tcatgtacca
     4921 tggcacattc cacagatatt ctacaatgcg gtggtctgca aaaggcactc ttgcttccag
     4981 tccggaatgc atgctggtac ggtccatacg gtctaataat gtctgcataa accatctcag
     5041 gttcagccac gcgatctcac ggcggcgggc ttcttctggt gtatcctctg ccagtctggg
     5101 tgtttctgct actgactttt cataagttgc ctgcacatat tcatccatgt gaagttcccg
     5161 gatgaaatca tcagaaagca gcacctttct ggctgaaaga tccatagtcc atggaaatgt
     5221 tttcgcctca aagcactctt ttttgtgaaa ccatggatag cccccaaata tctcatctgc
     5281 acattccccg gtcagagcca ccttatgggt acggctgacc tgggagcaga aatacaacaa
     5341 agatgagtct acatctgcca tggcaggcag atctctggct tctacagacg tataaagcag
     5401 atccgcctgg gtcacattgt cacattccag ataatgatga tctgaaccta aatatttcac
     5461 cataatgtcc acaaaaggcc ggtccataga cggctggaag gcattggcag taaaatattt
     5521 gtggttatcc ttaaaatcaa aagaaaaggt agtcagctgt ttatttttct tcttcagttc
     5581 tgcgctgcac actgctgata ccaggctgct gtccacacca ccggacaaaa aggtacacac
     5641 aggaacatcg gaaatgatct ggcggcagat ggcatcccgg accagaaatg ccgtcttttc
     5701 taccgtttgc tcgtaactgt ccgtatgagg cttactctct aatttccagt aacattctgt
     5761 atgcagacct gctgcattgc atacaagata gtgggccgga aggagttctt ctatgtcctt
     5821 gaaaacgccg catccatagg ttttcgcagg cccgatgctg aatatctcgt ttaaaccctt
     5881 tttatccagt tttggcgtaa cacccggata tgcgaaaagt cccttgatct cagaggaaaa
     5941 aatgacctgg tcatgaaaac aggtataaaa taatggcttt actcccagac ggtcacggaa
     6001 cagcaccagg cagtctttta ccgggtccat gatcccgaaa gcgaatatgc cgttcatctg
     6061 ctttacaaaa tccggaccgt attccatata tcctgtaagg accacttctg tgtcgccggt
     6121 agtggaaaac tcccagcctt ttttcagtaa atcttctttc aactctgtgg tattatacag
     6181 ttccccgttg tatacgatcc caaaggtctt atctcctgtt tttttaagga ttggctgacg
     6241 gccggaaacc agatcccgca ccgacagtct cacatgggct aacccaaaat gagagtccag
     6301 ataatttccc tgttcatccg gcccacgatg cttctgcact ttttccatat cttccagtat
     6361 ctgacggtag tgatcttcct ctgtgagata gtcaaaatga ctctgataaa atccggcgat
     6421 cccgcacata tgataacctc ctcttaaact caggttcaca cgctatcgcg ccctactgcc
     6481 tgcttgcgca ggcggaacct tacaacacaa gacacgccac cagaaaagtg catgtcaggc
     6541 caataaatac cggcagcata tttttccttg ccagttccac cggatctaca ttacagatag
     6601 ctgccaccgg tatcagtccc catggcacta ccgttccgcc accgataaaa atagcactga
     6661 tctgtcccag acatgccagg accggaatgg aagcgcctgt agctgtgcca aaggtcctcg
     6721 ccagggcacc tgtaagagga agcccggaaa agccggagcc atccagtcct gtaagaccac
     6781 cgatcaccag ctgcaataag gctgtcatat acctgtttaa aggagcatga tgggccagcc
     6841 atagcgccca gtcatttaaa attccacgct cataggagtc tcccaaaata tgggtaatac
     6901 cattgcctcc caggaagaaa aaggcaccga tcacaatgac cggagcaaaa atgcggatgg
     6961 caaataaaaa tccatctgtt acgtattccg tcaccttttc aagagaaccg gccttaaact
     7021 ccatgaccgc gcccacacac ataagaataa ctgcagtccc cgcaaccagg ctggtggcat
     7081 ctccgccctt taatttgaat aagaacataa acaggatatc cataagaaaa gcaatgggag
     7141 taagaaccgc cagagcctta gcagtggtgg aatattgctt cttcctatcc cccttttctt
     7201 ccggagtctc ttctccactg tcttcctgct tttcaggcag atccgttatt ttcagatcgt
     7261 tcctctgatc ttcatttttt ctctgtcctg ccaatgtcat ccggttgagc aaaaacgcgc
     7321 tgcatacaca ggtaataccc atgatccaga acagtggccg gcccttgctt aaaatatcgg
     7381 ttgtggaaat atctgccgct cccgcggaaa tggccggtgc cccctggatc actgcatcat
     7441 agctgagggc aaaaccatgg ccgaacaaat tcattgccat ggctgctgca agcggtgtta
     7501 atccggactg cacagcaatt ggaagtacaa tagcacctac cagtgccact gccggagatg
     7561 gccacaaaaa cagggaaaat acaaacatga tgccacctaa gatccaccag gaaacagcag
     7621 gagtcctcat aacttttgac accggcttca taagaagggc atcgcttccc aattcccgta
     7681 aacattttga aaatgccgtt accagggcaa tggtagcgat cacctcgcga aattcactga
     7741 tagcatagat aatggaactg aaaatggtct gtattcctcc tgccagactg cattttccaa
     7801 caacgccaat tccaaaaaga aaggcaatgc agaccaatgg cgtatctttt cgcaatatca
     7861 tggtaaataa gatcaccaac acccccacca ggtaaaacca gtggaccggt gttaatgcaa
     7921 tataagcttc aggcatataa aagctcctgt atttcatact tattaccatg gtatgcagct
     7981 tacaaaaaaa ggactctgcg ccagtgggaa acatcccacc ggactgcaaa atcctttgat
     8041 cttagactcg caaacccgcg ctgtcgcgct ctattgcctg ctgacgcagg ctgtttgcta
     8101 ttttctcaca tagaccattc catccatgat ccttcttgca gttctctgta ctgcaacacc
     8161 aggaagaact aattctccat tctcctcttt caggtccgga tacatggtca taacaccgct
     8221 tggatggccg atgcgcacat tggtctttcc ttcttccggc tttccaagct cttcatacag
     8281 cagacttcct tttaagaaag ctgctacgga aattgagctg gctgaagtca gagggcatgc
     8341 cttgtggcac ttaaatacag agatcacacg ggcgcaaacg tccatatcct ctgcatttac
     8401 ctgctgtcct gcaatatcca catagctctg tggctttgtg aaaaatccta cttttggcac
     8461 tgcagggctg ttagaagttg catcttctaa atcctttgca aagcccattt tgcagcaagc
     8521 tacaccgcgg atcttctcca atatttcact ggctgccttg ttgctgttta cctcttccgg
     8581 cagttcacgg cctgtaagac cgatatcctc tgcacgcacc aggaccattg ggttagacac
     8641 atccagaatg gtcgcctcaa tcggtccaaa tcccggaata tccagcacgt cctttggatt
     8701 tcctgttgga agcagctttc ctgtctttgc accggccgga tttaagaaat tgaccttcaa
     8761 ttcagctgct gttccgtcta cacctgcaat agcgcagtca ccctcctggg caaatgtctt
     8821 ggtttcagga tcgatcggaa cggttacatt gatatattta tctgtgttac ggtttagcat
     8881 acgaacagtg gtgatcggct ctacaatatc taccatacct tcttctactg cataaggtcc
     8941 tactgctgct gtcatatttc cacagttgga cttataatcc acctgactct tacctacaat
     9001 gacctggcct accagatatt ctacatctac acctggttct ttacttttcc atacaataac
     9061 gatcttattg ttggaagata cagtaccgcc aagaccgtcg atctgctttg gatccgggtc
     9121 tcccatgcac tgtaaaaaaa tgttatccca ttcttttctg tcttccggaa ggtcttcttt
     9181 atggaacata cagcctttgc tggtgccccc acgcataaat acagttttaa attttctcat
     9241 gtttcctgcc tctatttcag cttttcaatg gtctcaataa tataatcggt atattctttt
     9301 gcacttgcat cctctgtaaa agtagtcagt accactttct tctctgtggt acatacatcc
     9361 agtgcctttt ccagaagaac cttcttgtct gcatagccaa tatgagctaa catcatgcca
     9421 gctgcacgga tcagggagct tggatctgcg tactcgcctc tgccgtggct catcagatat
     9481 ggagcagttc catggatcgc ctcgaacatg gcatatttat taccgatatt ggaagaagaa
     9541 gcagttccca gaccgccctg atgctctgct gcaatatctg ttacaatatc gccgtacagg
     9601 tttggaagta ctactacttc aatacccttg ttaaactcag gatccagcat tttagcgcac
     9661 atagcatcta ctaaacgctc ctggatctcg atctccggat attcctcgcc taccttgcgc
     9721 actgccttga taaagtttcc atcagccagc tttacaatat ttgcctttgt tacaatggtt
     9781 acgttcttct taccattttt tcttgcaaac tcaaaagcag ctcttgcaat acgttcactt
     9841 ccctgcttgg tctgtacctt aaagtctact gccagatctt catctacctg gataccctta
     9901 tttccccaga tatattcacc ttcaatattc tctctgaaga aagtccagtc aatgcccttg
     9961 tccgggatac ggatcggacg gacagcagca aacagctcca ggccgcgacg cagcaggctg
    10021 ttagcagata ccaggtttgg ccatggctca ccggctcttg gagttaccat cggtcctttg
    10081 atgataacat cacatttttt gatctcttca aatacatcat ctggcagact ctgaagcttt
    10141 gcagctctgt tctcaatggt catgccttca atatggcgca gttctacttt gccggacgcg
    10201 atctcatccg gaatcagtgc cttgatcacg cggactgcct gctccataat gatcggacca
    10261 ataccgtctc ctggaaggat gcctacaacg atcttatcca gctttgaaaa atcttttact
    10321 tccgtatcct gtttcatacg ttcgatccgt tcaaactcag actggatcag ttccccaaat
    10381 ttttcctgtg cctgtgaaat agcgctcatt tttccttctc cttttctttc tttgaaatga
    10441 tcttaaattg attttaaatg gttgtatgtg tatgttactt ttttcacttt cttattttta
    10501 tattaataca aaaacagtaa taggtaaaat acattacata ccagttttta tatatgtaaa
    10561 atgcaacact atttttcaga attttcttta tatttcctta ttccagacac tcttttaatg
    10621 tacgcaaaaa ttccctggaa gcccttgaaa gtttctcatc agcaggataa gcagccacca
    10681 gcttccagga cgctcccagt tctttatcca gcggataaca gcttaatccc tctgtccccg
    10741 aatacagcgt catataagac tttggcagaa aagtagctcc cactccccct gccgccagac
    10801 gtttggctgt ttccatgctc tttgtagtgc agcagatatc cggccggatc ccggcttttc
    10861 ttaagatctc atctgccacc tggcgcagtt tctgtctgct tgccaccata acaaagggga
    10921 tctttttcag ttgccttata tcttctgcct ttaacgactt ttcctctgac agcgatacct
    10981 gtgactgaaa aatttccggc actgcaagat aaaaaggatc ctcagaaaat tcatcatagc
    11041 atacttcatc caatgctttc tgaaaatgca taatgcagaa gtccacttta tgagacagca
    11101 tcattttttc cagttcctgg gaattatttt cacggataaa tacctttacc cccggatagc
    11161 gcctgcgaaa cacaggaacc actacaggca ggatacaggc ccccaggttc atagggatgc
    11221 cga
//
LOCUS       DBMDUA010000085        11150 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2931_length_11150_cov_2.761155, whole genome shotgun sequence.
ACCESSION   DBMDUA010000085 DBMDUA010000000
VERSION     DBMDUA010000085.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11150)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2931_length_11150_cov_2.761155"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="ACZPCL_07825"
     CDS             complement(<1..901)
                     /locus_tag="ACZPCL_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013697.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1162409.1"
                     /translation="MLKKSLAIGLSVVMAAEVLTGCSGGSGTTETKAADTGKTEAAAS
                     EAATEGGSGEEKTLTVFHYMGQSVKQQGLEKLEEEFKKTHPNITFENIFYNQGTDYFP
                     QLSTALASGEQPDIIMGNPATYPDLIENGYAMDLTDNDVIKNLNVSPEDLNDASANGK
                     VYAVPIDFKASGFFYNVKMFKENGLEVPKTLTEFDQLVETLHSKGIDPFIDLYGEGST
                     GDIETRGIIVPRAVAAGDLDLYEKLMSGEKKLTDYDYMDEALSVWDKRMTYPRMDAMA
                     NNQDKALELFVTGQGAMIFTGNWN"
     gene            complement(1285..2361)
                     /locus_tag="ACZPCL_07830"
     CDS             complement(1285..2361)
                     /locus_tag="ACZPCL_07830"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1162410.1"
                     /translation="MEKQKKGVRLEHISKIYNDPKTGKEFYAVQDTTLDIEPGTFVTL
                     LGPSGCGKTTTLRMIAGFESPDEGEIYLGDEAINSLTPNKRDTAMVFQSYALLPHYNV
                     FDNVAYGLKIRKVPKEEIRERVLNILKLVEMDGMESRMTNQLSGGQQQRVALARALVI
                     EPSVLLFDEPLSNLDAKLRVTMRTEIRKIQQKVGITAIYVTHDQSEAMSISDKIIIMS
                     KGKVEQIGTPREIYYHPKSRFVADFIGEANFLKAKVKSVNGEKAVIDVIGEEIEVNNY
                     GEKAAGEEASLVLRPEAVVLSEKGLLEGTVTLSTFMGSYQYYQVMVGDMEIQITDYNP
                     VNRRIYEVGEKAYLDFDPHGVYIL"
     gene            complement(2365..4074)
                     /locus_tag="ACZPCL_07835"
     CDS             complement(2365..4074)
                     /locus_tag="ACZPCL_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1162411.1"
                     /translation="MAGKQSAQLERKKFFADPIMVSMIVVLLVFLALFILYPLLMLLV
                     DSFYAEGTITLDVFKRVLNLERFRNAFKNTLTLGFIVGFASTAIGLLFAYVEVYVKLK
                     TKILEKLFGLVSMLPVVSPPFVLSLSMIMLFGRSGIITRSLLGIYDSNIYGLLGISVV
                     QTLTFFPVCYLMLKGLLKNIDPSLEEATRDMGATRWKVFTSVTFPLLLPGLGNAFLVT
                     FIESVADFANPMLIGGSYDTLATTIYLQVTGAYDSTGAAAMSVVLLSLTVILFLIQKY
                     YLEKKTAATLTGKASRMRMLIEDKSVTVPLTIFCGVVAAFVLLMYIMVPFGALFTLWG
                     RDYSLSLKWFQYMFKTSGLKAFKDSFVLSLIAAPLTAFLSMVISYLVVKKRFKGRNFI
                     EFVSMLAMAVPGTVLGIGYIRGYANGLFRSGFMSGLYGTGAILIIVFIVRSLPTGTRS
                     GISALRQIDKSIEESAYDMGANSARVFMTVTLPLIKDSFFSGLVTAFVRSITAISAII
                     LLVTPDFLLITCQINEQAEKGNYGVACAYATVLIIITYGAVLIMNTLMKFFGVSRKIK
                     TEE"
     gene            complement(4246..5430)
                     /locus_tag="ACZPCL_07840"
     CDS             complement(4246..5430)
                     /locus_tag="ACZPCL_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1162412.1"
                     /translation="MKKKLALLLACTMALGLTACGGSKPAAAPETTAAATEAAAESSE
                     ESKADEKTEAAGGDMDALIEAAKAEGELTVYGSCEEEYLSAACQNFEKLYGIKTKYQR
                     LSTSEVYTKVSEEAGKPSADVWFGGTMDPYNEAVAAGLLEPYDAINAVNLIGDEYKDP
                     TNCWYGIYKGILGFMVNTEELERLGLEAPKTWDDLTKEEYKGLIMMSNPNTAGTAKLI
                     INTMIQMKGHDEAMEYFKALDKNIAQYTKSGSGPSKMVGPGECVIAIGFLHDGIYQIL
                     QGYDNIQLIVPEDGTSFEVGATAILKGASHPNAAKLWIEYALSPDCVDHAKENGSYQF
                     LVLKNATQPEEAEKFGLDMTNTIDYDFEDAKENSAKYVEDFFEALGSTSEGADSSRFL
                     TE"
     gene            complement(5608..6846)
                     /locus_tag="ACZPCL_07845"
     CDS             complement(5608..6846)
                     /locus_tag="ACZPCL_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR1162413.1"
                     /translation="MADEKMIDRAEHVLYKTYNRFPVVFDHGKGITLWDTEGNEYLDF
                     GAGIAVMGLGYCDEEYTNAVKAQMDKLTHISNLFYNQPSISAGEKLLKVSGMDKVFFT
                     NSGTEAIEGALKIAKRYHYNKLHETMGDGCDGCEEKEVDMTGEIIAMNHSFHGRSIGA
                     LSVTGNAHYRTPFNPLLPGVRFANFNDLDSVKAQITDKTCAIIMETIQGEGGIYPATE
                     EFLKGVRALCDEHDLLLILDEIQCGMGRSGEMFAWQKYGVKPDVMTVAKALGNGVPIG
                     AFLACGKAATAMVPGDHGTTYGGNPLVTAAADAVLDIFEKRHIADHVKEVGAYLYEKL
                     EGLKDKYDVVKDHRGVGLIQGLEFTVPVGPIVSKALLEQKLVLISAGANIIRFVPPLI
                     IEKSDVDEMVKRLEAVIKEM"
     gene            complement(6839..7729)
                     /gene="argB"
                     /locus_tag="ACZPCL_07850"
     CDS             complement(6839..7729)
                     /gene="argB"
                     /locus_tag="ACZPCL_07850"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR1162414.1"
                     /translation="MNLEPMQKAAVLIEALPYIQRFNRKIVVVKYGGSAMVDEELKKK
                     VIQDVVLLKLVGFKPIIVHGGGKEISRWVGKVGMEPHFVNGLRVTDEPTMEIAEMVLN
                     KVNKSLVQLVNELGIKAVGISGKDGMMLKCHKKLSKGGDIGFVGEVDEVDPQVIYDLL
                     EKDFLPIICPIGYDENFLSYNINADDAACAIARAVKAEKLAFLTDVEGVYKDFEDKSS
                     LISEMTVEEAQAFVDSGSLGGGMLPKLQNCIDAIENGVSRVHILDGRIPHCLLLEIFT
                     DKGIGTAIYNSKEDVRYYHG"
     gene            complement(7823..9058)
                     /gene="argJ"
                     /locus_tag="ACZPCL_07855"
     CDS             complement(7823..9058)
                     /gene="argJ"
                     /locus_tag="ACZPCL_07855"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR1162415.1"
                     /translation="MNEKIKMIEGGVTAAKGFMAASTAAGIKYKNRQDMAMIYSKEPC
                     KSAGTFTTNLVKAAPVKWDKNQVTSGADAQAVIINAGIANACTGEEGMGYCAQTAKAA
                     AETLGVAEDGVLVASTGVIGMQLPIDKIAAGVKAMVPLLGDSLEKGTEASKAIMTTDT
                     KNKQVAVQIDMNGTTVTIGGMCKGSGMIHPNMCTMLSFVTTDAAISKELLQEALSEDI
                     KDTYNMISVDGDTSTNDTVLLLANGLAGNKEITEKNEDYALFCEALNYINETLAKKMA
                     GDGEGCTALFEVKIVGAETKDQAKVLAKSVITSSLTKAAIFGHDANWGRILCAMGYSG
                     AQFDPEKVDLFFESAAGHMQIIKDGVAVDYSEEEATKILSEPEVTAIADIKMGDAQAT
                     AWGCDLTFDYVKINADYRS"
     gene            complement(9112..9576)
                     /locus_tag="ACZPCL_07860"
     CDS             complement(9112..9576)
                     /locus_tag="ACZPCL_07860"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1162416.1"
                     /translation="MAGTMDILVREMTMEDYDQVYKLWTKISGFRIRSIDDSREGVER
                     FLKRNPTTSVVAVQNGHIVGNILCGHDGRTGCFYHVCVEDGYRHHGIGYKMVRAAIKA
                     MQKEGVSKISLVAFKDNHIGNAFWQGIGWTEREDFNSYEFILNEENIIRFVK"
     gene            complement(9602..10642)
                     /gene="argC"
                     /locus_tag="ACZPCL_07865"
     CDS             complement(9602..10642)
                     /gene="argC"
                     /locus_tag="ACZPCL_07865"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR1162417.1"
                     /translation="MIKAGIIGSTGYAGGELARLLLQRDDVEIKWYGSRSYIDQKYAS
                     IYQNMFKIVDDKCMDDNMKELADQVDVIFTATPQGLCSTLVNEEILSKVKIIDLSADF
                     RIKDVAVYEKWYKLTHGSPEYIKEAVYGLPEVNREKIKNARIIANPGCFPTCSFLSTY
                     PLVKEGLIDPNTIIIDAKSGTSGAGRSAKVDSLFCEVNENIKAYGVASHRHTPEIEEQ
                     LSYAAGKPVVISFTPHLVPMNRGILVTAYASLTKEVTWEEVRAAYDKYYKDEYFVRVL
                     DKDVVPQTRWVEGSNFADVNFKIDPRTNRIVMMGAIDNMVKGAAGQAIQNMNLLFGLP
                     ENTGLKQIPMFP"
     gene            10958..>11150
                     /locus_tag="ACZPCL_07870"
     CDS             10958..>11150
                     /locus_tag="ACZPCL_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HIR1162418.1"
                     /translation="MKEKVVLAYSGGLDTTAIIPWLKEHFNYDVVCCCIDCGQGKELE
                     GLEERAKLSGASKLYIEDLV"
BASE COUNT         2818 a         2641 c         2221 g         3470 t
ORIGIN      
        1 tattccagtt accggtaaag atcattgccc cctgtccggt cacaaacagc tccagagcct
       61 tatcctgatt gtttgccatt gcatccatac gtggataagt cattctctta tcccatacgc
      121 ttaatgcttc atccatgtaa tcgtaatccg tcagcttctt ttcaccgctc atcagcttct
      181 catacagatc cagatcaccg gcagctacgg ctcttggaac aatgatacct cttgtttcaa
      241 tatcaccggt agaaccttct ccgtacaggt cgataaatgg atcaatgcct ttgctgtgta
      301 atgtttcaac taactggtca aattctgtaa gggtctttgg aacttccaga ccattttcct
      361 taaacatctt tacattgtag aaaaagccgg atgccttaaa gtcaataggt actgcatata
      421 cttttccgtt tgcggaagca tcatttagat cttccgggga aacatttaaa ttcttgatca
      481 catcattatc cgtcagatcc attgcatatc cgttttcgat cagatccgga taggtagccg
      541 ggttacccat gataatatca ggctgttctc cagatgccaa agctgtggat aactgtggga
      601 agtaatctgt tccctgatta tagaagatat tttcaaaggt aatgtttgga tgtgttttct
      661 taaattcttc ttccagtttt tcaagaccct gctgtttcac ggactgtccc atatagtgga
      721 acacagtaag agtcttttct tctcctgaac cgccttctgt tgctgcctcg cttgctgctg
      781 cttctgtttt tccagtatca gctgccttgg tctctgttgt gccagagccg ccggaacaac
      841 cggtaagtac ctctgctgcc ataaccacac tcagccctat tgccaatgac tttttcaaca
      901 tactttattt cctccttctt actatgttct ctcgaaatct ttctgtgcct gattttgtga
      961 gaagattttt tgtcagttgt tcccaaattt ctgaggcgta cgcggtgcgt acgttgatga
     1021 aattcgggtg cgactggcgg aaaatcggcc acaaaggcag gtgcagtaaa gacttcgaga
     1081 ggacatttat taatatttct gatttctttg atagttctta ttatacagaa aaaccatctg
     1141 ttttaatatt tatcagattt cagaaaatat atatcaggtt tatcatgttt ttagatacat
     1201 agcaattcgg ggtggcaata aaaaagcgta tccaaagttt atattttctc aggatacgct
     1261 tttataagtc gctagattac tattttacag gatataaaca ccatgaggat caaagtccag
     1321 atatgccttt tcacctactt catagatccg gcggtttaca ggattgtagt ctgtgatctg
     1381 gatctccata tcacctacca ttacctggta atactgatag gaacccatga aggttgacag
     1441 tgtaacagtt ccttccagaa gtcctttttc agaaagaaca actgcttccg gacggagaac
     1501 caggcttgct tcctcaccgg cagccttctc accatagttg ttgacttcga tctcttcacc
     1561 gattacatcg atcacagcct tttctccatt cacagactta acttttgcct ttaagaagtt
     1621 ggcttcacca ataaagtctg ctacgaaacg gctctttgga tgatagtaga tctctcttgg
     1681 agtaccaatc tgctctactt tacccttgct cataataatg atcttatcag aaatactcat
     1741 tgcctctgac tggtcatggg ttacgtagat agcagtaatt cccaccttct gctggatctt
     1801 acggatctca gtacgcatgg tcacacgcag ctttgcatcc aggttactta aaggctcatc
     1861 aaaaagcaaa acacttggtt caatgaccag agcccgggcc agagcaactc gctgctgctg
     1921 tccaccggaa agctggttgg tcatacgtga ctccattccg tccatttcta ccagtttcag
     1981 gatgtttaaa actctctctc tgatctcttc ctttggcacc ttgcggatct ttaagccgta
     2041 agctacgtta tcaaatacat tgtaatgtgg cagcagagcg tagctctgga ataccattgc
     2101 agtatcacgt ttatttggtg tcaggctgtt gatggcctca tcacctaagt agatctcgcc
     2161 ttcatcgggg ctttcaaaac ctgcgatcat acgcagtgta gtcgtttttc cgcagccgga
     2221 aggtcccagt aaggtaacaa atgtacctgg ttcaatatcc agtgtggtat cctggactgc
     2281 ataaaactct tttcctgttt taggatcatt atatatttta gaaatatgtt ccagacgaac
     2341 accttttttc tgcttttcca taacctattc ctccgttttg atttttctgc tgactccgaa
     2401 gaatttcatc agcgtgttca tgatcagcac tgcaccgtag gtaatgatga tcagtacggt
     2461 tgcataagcg caggcaacac cgtagtttcc tttttctgcc tgctcgttga tctgacaggt
     2521 gatcagcagg aagtcaggag ttactaacag gatgatggct gaaatagcgg taatactgcg
     2581 gacaaacgca gttaccaggc cgctgaagaa ggaatcctta ataaggggta atgtaacagt
     2641 cataaatact cttgctgagt ttgcacccat gtcgtaggca gactcttcaa tggatttatc
     2701 gatctggcgc agagcggaga taccgcttcg tgtaccggta ggaaggcttc gaacgataaa
     2761 tacaatgatc aggatagcac cggttccata aaggccgctc ataaagccgc ttcggaacag
     2821 accatttgca taaccacgga tatatccgat accaagaacc gtaccaggta cagccattgc
     2881 caacatagat acgaattcaa tgaagtttct tcctttaaag cgcttcttaa caaccaggta
     2941 ggaaatgacc atggataaga acgcagtaag aggagctgcg atcagggaca gtacgaagga
     3001 gtctttaaat gccttcagtc cgctggtctt aaacatgtac tggaaccatt taaggcttaa
     3061 gctgtaatct ctgccccaaa gggtgaacag tgcgccgaaa ggaaccatga tgtacatcaa
     3121 aagtacgaaa gctgctacca cgccgcagaa aatagtcagc ggaacggtta cgctcttatc
     3181 ttcgataagc attctcatac gggaagcttt accagtaaga gtagcggctg ttttcttctc
     3241 caggtaatat ttctggatca ggaacagaat aacagtcagt gaaagcagga ccacagacat
     3301 ggctgctgca ccggtggagt cgtaagcacc tgttacctgc agataaatgg tagttgccag
     3361 agtatcgtag gaaccaccga tcagcattgg gtttgcaaag tctgctacag actcaataaa
     3421 ggttaccagg aatgcatttc caagacctgg aagcagaagt gggaaagtta cgcttgtgaa
     3481 tactttccaa cgggtagctc ccatatctct ggtcgcttct tccagtgacg ggtcaatgtt
     3541 ctttaacagt ccctttaaca tcagatagca tacagggaag aatgtaagtg tctgaacaac
     3601 ggagatacct aacagtccgt aaatattaga gtcgtagata cctaaaaggg aacgggtaat
     3661 gataccgctt cgtccgaaca gcatgatcat ggacagagac agtacgaatg gcggagatac
     3721 aacaggaagc atagatacca gaccaaataa cttttccagt atctttgttt tcaatttaac
     3781 atatacttct acataagcaa ataataaacc aatggctgta gaagcaaaac ctacgataaa
     3841 tccaagtgtc agtgtgtttt taaacgcatt gcggaaacgt tccagattta aaacacgctt
     3901 aaatacatcc agtgtaatgg ttccctctgc ataaaagctg tctactaaca gcattaacag
     3961 cggatacaga ataaacagcg ccaaaaaaac cagaaggaca acaatcattg agaccatgat
     4021 cgggtcagca aaaaatttct tccgttccag ctgtgcactt tgttttccgg ccattgcgag
     4081 accccctttt ataatttact atgaatttta caaccaattc tgtctttgat ttgaaaatgc
     4141 tgcgcaatgt ttattccatt acgcagcatt ttctgcttag cttactttat ataagcttta
     4201 ctttcttttt aagaatgact ttttgcagtc cgtctattat tctgattact cagttaagaa
     4261 acggctggag tcagcgcctt cagatgtgct tcctaatgct tcgaagaagt cttctacata
     4321 cttagcactg ttctcttttg catcttcaaa gtcatagtca atggtattgg tcatatccag
     4381 accgaacttt tcagcttctt ctggctgagt tgcgttcttt aataccagga actgataaga
     4441 accattctct tttgcatggt ctacacagtc aggagataat gcatactcga tccacagttt
     4501 tgctgcattt ggatgagaag cgccctttaa gatagcggtt gcaccaactt cgaaggatgt
     4561 tccgtcttct ggaacgatca gctggatgtt gtcatatccc tgaaggatct gataaatacc
     4621 atcatgtaag aaaccaatag cgattacaca ctcgccaggg ccaaccatct tggaaggacc
     4681 ggaaccggac ttggtgtact gggcaatgtt cttgtccaga gccttgaagt attccatagc
     4741 ttcgtcatgg cccttcatct ggatcatggt gttgatgatc agtttagcag taccagcggt
     4801 attcgggttg gacatcatga tcaggccctt gtactcttcc ttggtcagat catcccatgt
     4861 cttaggtgct tccagaccta aacgctccag ctcttctgta ttgaccatga agcccaggat
     4921 gcctttgtag ataccatacc agcagttggt aggatctttg tactcatcac cgatcaggtt
     4981 tacagcgttg atcgcgtcat atggttccag cagaccagct gcaactgctt cgttgtaagg
     5041 atccatggtt ccgccgaacc atacatctgc tgaaggcttt ccagcttctt cagaaacctt
     5101 ggtgtaaact tctgaagtag aaagtctctg gtattttgtc ttaattccgt agagtttttc
     5161 aaagttctgg caggctgctg ataagtattc ctcttcgcag gaaccatata cagttaattc
     5221 gccctcagcc ttagctgctt cgatcagagc atccatatcc ccgccggctg cttctgtctt
     5281 ctcatcagct ttgctctctt cgctgctttc tgcggctgct tcggttgctg ctgcagttgt
     5341 ctccggagct gctgcaggtt tggaaccgcc acatgctgtc aggcctaacg ccatggtaca
     5401 agccagtaac aatgcaagtt ttttcttcat aataaaaacc ctcctgtctt ttctgttgac
     5461 ttaagtctac cagaaatcag gaggggaaaa aactgtattt tttgttattt tgtcgtattt
     5521 tctgaactat gtagttcatt attcgtgcat ttttgccact ttttctcaac taccggactc
     5581 tttgcgttcg ttatgagaac tttttcacta catttctttg atcacagctt ccagtctctt
     5641 aaccatttcg tctacatcgc tcttttcaat gatcagcggc ggaacgaaac ggataatatt
     5701 ggcgcctgca ctgatgagca ccagcttctg ctctaataat gccttggaca cgatcggacc
     5761 tacaggaact gtaaattcca gaccctggat caggccaaca ccacggtggt cttttaccac
     5821 atcatactta tcctttaagc cttccagctt ttcatacaga taagcgccaa cttcctttac
     5881 atggtctgca atgtggcgtt tttcaaagat atctaaaact gcgtctgctg cagctgttac
     5941 cagtggattt ccgccatagg tagttccatg gtcacctggg accatagcag tagctgcctt
     6001 accgcaggct aagaatgcac cgatcggaac accatttccc aaagcctttg caacggtcat
     6061 tacatctggt tttacgccgt atttctgcca tgcaaacatt tctccggaac gtcccatacc
     6121 acactggatc tcatctaaaa taagcagcag atcatgttca tcgcagagag cgcgcacgcc
     6181 ctttaagaat tcttctgttg caggataaat tccgccttcg ccctggatag tctccataat
     6241 gatagcgcag gtcttgtctg taatctgtgc ctttacgctg tccagatcat taaagttggc
     6301 aaaacgtaca cctggaagca gtggattaaa cggagttctg taatgtgcat ttccagttac
     6361 agacaaagca cctatacttc ttccgtggaa ggaatggttc atggcaatga tctcaccagt
     6421 catgtccact tctttttcct cacagccatc gcagccgtct cccattgtct catgaagttt
     6481 attataatga tatctctttg cgatctttaa tgcgccttca atagcctctg taccgctgtt
     6541 ggtaaagaat accttatcca ttccggatac ctttaacagc ttttcgcccg cactgataga
     6601 aggctggtta taaaacagat tggagatatg ggtcagctta tccatctggg cctttactgc
     6661 atttgtatac tcttcatcac agtatcccag tcccataact gcaatacctg caccaaaatc
     6721 caggtattca tttccttctg tatcccacaa ggttatgcct tttccatggt caaatactac
     6781 aggaaaccgg ttatatgttt tatacaggac atgttctgcc cgatcgatca ttttctcatc
     6841 agccatgata atacctcaca tcttcctttg aattataaat agcagtacca atacctttat
     6901 ctgtaaagat ctccagaagc aggcagtgtg gaatacgtcc gtctaagata tgtactctgg
     6961 aaacaccatt ttcaatggca tcaatacagt tctgcagctt tggaagcatg ccgccgccta
     7021 agcttccgct gtctacaaag gcctgagcct cttctacggt catttcagag ataagggaac
     7081 tcttgtcctc aaaatccttg tatacacctt ctacgtctgt taagaaagcc agcttttctg
     7141 cttttacagc tcttgcaatg gcacaggctg catcatctgc gttaatgtta tagcttaaga
     7201 aattctcgtc atatccaatt gggcagatga tcggaaggaa atctttctct aacagatcat
     7261 agatgacctg aggatctacc tcatctactt cccctacaaa gccgatatct cctcccttgg
     7321 aaagcttctt gtggcatttt aacatcatgc cgtctttgcc gctgatgcct actgccttga
     7381 tgcccagctc atttaccagc tgtaccaggc tcttattgac tttatttaat accatttctg
     7441 caatttccat ggttggctca tcggttacac gaagaccatt gacaaaatgt ggctccatgc
     7501 ctacttttcc aacccagcgg ctgatctcct tgccgccgcc gtgtacgatg atcggtttaa
     7561 atcctactaa tttcaacagt actacgtcct ggatgacttt tttctttaat tcttcgtcta
     7621 ccatggcgct gccgccgtat tttacaacga cgatctttcg gttaaaacgc tgtatgtatg
     7681 gcagtgcctc aatgagcacc gctgcttttt gcattggttc aaggttcatg tttggttcct
     7741 cctgtatgtt ttgtgtacgt ccaaatgttc atggggtaca tttcgggatg cttttttatt
     7801 gtgtttttta tatcaaaact gtttatgaac ggtaatctgc attgatcttt acataatcaa
     7861 aggtcaggtc gcagccccat gcagttgcct gggcatcacc cattttaatg tctgcaatgg
     7921 cggtaacttc cggctcagaa aggatcttgg ttgcctcttc ttcgctgtag tccactgcaa
     7981 cgccgtcttt gatgatctgc atatgtccag ctgcgctttc aaagaacaga tctacctttt
     8041 ccggatcaaa ctgtgcccct gagtagccca ttgcgcaaag gatacggccc cagtttgcgt
     8101 catgtccgaa aatagctgcc tttgtcaggc tggatgtgat aacagatttt gctaaaactt
     8161 tcgcctgatc cttcgtttct gcgcctacga tctttacttc aaataaagcg gtgcagcctt
     8221 ctccgtcacc ggccattttc ttagccagag tttcgttgat gtagtttaat gcttcgcaga
     8281 ataatgcgta atcttcattt ttctctgtaa tttccttgtt tcctgcaagg ccatttgcca
     8341 gaaggagaac ggtatcattg gtggaagtat caccatctac agaaatcata ttgtaggtat
     8401 ctttaatgtc ttcgcttaat gcttcctgaa gcagttcttt gctgatggct gcgtccgtag
     8461 taacaaagct taacatggta cacatatttg gatggatcat gccggaacct ttgcacatac
     8521 cgccaatagt aacggtggtt ccgttcatgt cgatctgtac tgctacctgc ttgttctttg
     8581 tatcggtagt cataatagcc ttggaagcct cggtgccttt ctccaggctg tcaccaagca
     8641 gtggcaccat agcctttaca cctgctgcga tcttgtcgat tggaagctgc ataccgatga
     8701 caccggtaga agcaactaaa acgccgtctt ctgccacgcc taaggtttct gctgcagcct
     8761 ttgccgtctg tgcgcagtag cccattcctt cttcaccggt acatgcattt gcaatacctg
     8821 cgttaatgat cactgcctgt gcgtcagcgc cgcttgtcac ctggttcttg tcccatttta
     8881 caggagctgc ttttaccagg ttggtggtaa aggttcctgc acttttacaa ggttctttac
     8941 tgtagatcat cgccatatcc tgacggtttt tgtatttaat gcctgctgct gtagatgctg
     9001 ccataaagcc tttggctgcg gtaacgccgc cttcgatcat ttttatcttt tcgttcataa
     9061 tattattccc tcttttctgc ttcgcaactg tttgtttttc tctgcgccac tttacttcac
     9121 aaaccgaatt atattttctt catttaaaat aaactcataa ctgttaaaat cttcacgctc
     9181 tgtccagccg atgccctgcc agaacgcatt tccaatgtgg ttgtctttaa aggccaccag
     9241 ggagatcttg ctcactccct ctttctgcat ggccttgatg gctgcacgta ccattttgta
     9301 gccaattcca tgatgacggt agccgtcttc cacacataca tgatagaaac agccggtacg
     9361 tccgtcatgt ccgcagagga tatttccaac gatatgcccg ttctggactg ccactacgct
     9421 ggtggtcgga tttcgcttta agaagcgttc caccccttct cttgaatcat caatgctccg
     9481 gatacggaag cctgatatct tcgtccataa tttatatacc tgatcgtaat cttccatggt
     9541 catttcccga accaggatat ccattgtccc tgccattttt attcctttcc ggctgcttat
     9601 cttatgggaa catcgggatc tgctttaatc ctgtattctc tggaagaccg aacagtaagt
     9661 tcatgttctg gatggcctgt cctgcagcac cttttaccat attgtcaatt gcgcccatca
     9721 taacgatgcg gttggttctt gggtcgatct tgaagtttac atctgcaaag ttgcttcctt
     9781 ctacccagcg tgtctgagga actacgtcct tgtccagaac acgtacaaaa tattcatcct
     9841 tataatactt gtcgtaagcg gctcttacct cttcccaggt cacttctttt gtaagggaag
     9901 cataagcagt taccaggatg cctctgttca ttggaaccag atgaggggtg aagctgatca
     9961 caaccggctt tcctgctgca taggacagct gctcttcgat ctccggtgta tgtctgtggg
    10021 aagctacgcc gtatgccttg atattttcat tgacctcgca gaaaaggctg tctactttcg
    10081 cgcttctgcc tgcgccggag gtaccggatt ttgcatcaat gataatggtg tttggatcaa
    10141 tgagaccttc ttttactaaa ggataagtag ataagaagga gcaggttggg aagcatcccg
    10201 gatttgcaat gatccttgca ttcttgatct tttctctgtt tacttccgga agtccgtata
    10261 cagcttcctt aatatattct ggtgatccgt gggtcagctt gtaccatttt tcataaactg
    10321 ctacatcttt aatacggaag tcagcgctta agtcgatgat ctttacttta gaaagaatct
    10381 cttcgtttac taatgtggag caaaggccct gaggcgttgc agtgaagatt acatctacct
    10441 ggtcagccag ttccttcata ttatcatcca tacatttatc atctacgatc ttgaacatgt
    10501 tctggtaaat ggaagcatat ttctgatcga tataacttct ggaaccatac catttgatct
    10561 ctacgtcatc tctctgcagc agcagccttg ccagttcccc gcctgcataa cctgtagatc
    10621 cgataatacc tgcttttatc atatccttac tctcctctgt atatattttc attatttagt
    10681 catttttata aactgcaacc tactacagtg ctaaagcctg ttaccttatt atagttactt
    10741 tttccttaat tgaaaagtgt gttttcataa ggttttttat tttttctctg tataagattt
    10801 cataattata catctgcaat gcataatatg caatactttt ttgaaatttc tattttaagg
    10861 cttgcatttt cctgtattat gttgtatatt tatgaaagct tagtgattaa aacatgatca
    10921 aaacaaatat caaagcaatt ttcaggagga tgtactgatg aaagagaaag tagttttagc
    10981 atattcaggc ggtctggata ccaccgctat cattccgtgg ttaaaggagc atttcaacta
    11041 tgatgttgtt tgctgctgta tcgactgtgg acagggaaaa gaattagagg gactggaaga
    11101 aagagcaaag ctgtccggcg cttctaaatt atatattgaa gatttagtag
//
LOCUS       DBMDUA010000086        10988 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2957_length_10988_cov_2.450170, whole genome shotgun sequence.
ACCESSION   DBMDUA010000086 DBMDUA010000000
VERSION     DBMDUA010000086.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10988)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10988)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10988
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2957_length_10988_cov_2.450170"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            152..2662
                     /locus_tag="ACZPCL_07875"
     CDS             152..2662
                     /locus_tag="ACZPCL_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR1162419.1"
                     /translation="MERYTPQAKEALSLAVGMAESLNHGYVGTEHLLIGLLQEGTGVA
                     ARVLEENGVEESKVVELVSQLISPNTSVQMAENAAYTPRARRVIENSYREAVRFKAAQ
                     IGTEHILIAILREGDCVASRLLNTMGISVQKLYIDLLAAMGEDAPSIKDEMQRGNSGK
                     RGSSTPALDSYSRNLTQMALDGKLDPVIGREHEIQRVIQILSRRTKNNPCLIGEPGVG
                     KTAVVEGLAQRIAAGDVPDTIADKRVMTLDLSGMVAGSKYRGEFEERIKKVIAEVVEA
                     KDVLLFIDEIHTIIGAGGAEGALDASNILKPSLARGELQLIGATTINEYRKYIEKDSA
                     LERRFQPVTVDEPSEEESIAILKGLRSRYEEHHRVEITDDALEAAVKLSSRYINDRFL
                     PDKAIDLIDEAASKVRLSNYTKPSKIKDYEAQIDDLEEEKESAIRDEAYEKAGDIKKK
                     QEKLKEKIRLTLEKWEKEKETRKLVVGENEVADVVAGWTKIPVKKLAQEESERLKNLE
                     GILHERVVGQEEAVTAVSKAIRRGRIGLKDPKRPIGSFLFLGPTGVGKTELSKALAEA
                     MFGTESALIRVDMSEYMEKHSVSKMIGSPPGYVGYEEGGQLSEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQVLDDGHITDAQGRKIDFKNTIIIMTSNAGAENIIAPKRLGFGVAT
                     DAKADHEFMKGRVMEEVKRLFKPEFLNRIDEIIVFHQLTKEHMKGIADIMLRGIEKRS
                     KEQLGITLTVNEAAKDLLIDKGYDDKYGARPLRRTIQSLLEDKMAEEILDGKLKKGVN
                     VEVGCEEGKLTFTVKKKAAARKKKAQAADTASKPEAKA"
     gene            2821..3237
                     /locus_tag="ACZPCL_07880"
     CDS             2821..3237
                     /locus_tag="ACZPCL_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endosialidase"
                     /protein_id="HIR1162420.1"
                     /translation="MPVVEELIRTEQDGTISFGNYKLGQKSKKSDFEYQGDIYKVKTY
                     NEITKLERNEMFVYESVPGTVAEHFKVTDDSIEFEVEGDKDAQITVQLENDTDYEVYV
                     DGAAVGSMKTNMSGKLSVSVELEEGTSVKVKAVKRA"
     gene            3243..4625
                     /gene="radA"
                     /locus_tag="ACZPCL_07885"
     CDS             3243..4625
                     /gene="radA"
                     /locus_tag="ACZPCL_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR1162421.1"
                     /translation="MAKAKTTAFFCKECGYESSKWLGQCPACRAWNTLVEEPVAGTVS
                     VAGKGRMSSDRGAGRAVAKPALLSEIRLEQEDRISTGFKELDRVLGDGIVAGSLVLVG
                     GDPGIGKSTLLLQVCRHLAAAGQKVLYISGEESLKQIKMRANRIGTVTGELRFLSETN
                     LERIEAVIDQEKPQIVVIDSIQTMYREEITSAPGSVSQVRESTGVLMQIAKSQGIAIF
                     VVGHVTKEGVVAGPRVLEHMVDTVLYFEGDRHASYRILRGVKNRFGSTNEIGVFEMRE
                     EGLVEVENPSEYMLDGRPQEASGAVVSCSLEGTRPILLEVQALVAETNFGMPRRTAAG
                     TDYNRVNLLMAVLEKRCRYELSRLDAYVNITGGMRMSEPALDLAIVMALMSSYKDRPV
                     DPDMLIFGEVGLSGEVRGVSQAAQRVAEAAKLGFTTCVLPKVNMDKIKPVEGIRMIGV
                     SNVREAIGLL"
     gene            4848..4918
                     /locus_tag="ACZPCL_07890"
     tRNA            4848..4918
                     /locus_tag="ACZPCL_07890"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4880..4882,aa:Trp,seq:cca)
     gene            5025..6179
                     /locus_tag="ACZPCL_07895"
                     /pseudo
     CDS             5025..6179
                     /locus_tag="ACZPCL_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574357.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4317 domain-containing protein"
     gene            6486..6917
                     /locus_tag="ACZPCL_07900"
     CDS             6486..6917
                     /locus_tag="ACZPCL_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD1 domain-containing DNA-binding protein"
                     /protein_id="HIR1162422.1"
                     /translation="MSRTIKAGHVYRQYSKDGMPIADYKSSRLAHEATGVSLGSIARA
                     AHGERKTGGGYLWRVVPEDLPWEPIQVDLSSKIGYHDKRPLIQLTLDGQVVAEYLSIA
                     HASRALNISRRSISCAMTGAQKTAGGYKWVEKETPETKEKE"
     gene            7180..7422
                     /locus_tag="ACZPCL_07905"
     CDS             7180..7422
                     /locus_tag="ACZPCL_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1162423.1"
                     /translation="MKEKKIMLPSVEEAKHFVAEATKCDFDIDVFYNRVTIDAKSILG
                     VLSLDLARVLTVQMNGDNAEFEEYLDTLAPVSTTAA"
     gene            7579..10029
                     /locus_tag="ACZPCL_07910"
     CDS             7579..10029
                     /locus_tag="ACZPCL_07910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR1162424.1"
                     /translation="MTEAQSKIRISVRNLVEFVLRSGDLDNRRSQGGKKEAMQAGSRL
                     HRKIQKRMGADYRSEVALKHQVREDEFSILLEGRADGIITEMSGIVIDEIKCIYMDVE
                     RLEEPDPVHLAQALCYGYMYASEQDLDSVGIQLTYCNIETEQIRRFRQAKTREELEEW
                     FQGLIHEYVKWARYLYHHSIRRTECLKELQFPYAYREGQKELAVSVYRSIARGRNLYI
                     QAPTGIGKTLSCVFPSLKAIGEGYGEKLFYLTAKTITRSVAEETFELLRERENLYFST
                     VTITAKEKLCILEKPDCNPVACPRAKGHFDRVNDAVYEIIQEEQGITRETILAYAEKY
                     QVCPFELCLDISSWVDGIICDYNYVFDPNVRLKRYFAEGEERGNYIFLVDEAHNLVSR
                     AREMYSAVLIKEELLAAKRILKGIPEAGKVLKLLERCNRRMLDLKRLGETEPFSDEAD
                     MELAFEGGARMVLGEHYRMHSDVKLLSLELMGLFGEMEVFLNENSEFDDRETVLDLYF
                     KIRDFLYVSDRLDENYRIYSRLLADGSFMVKLMCVNPSSCLRECLDRGVSTVFFSATL
                     LPIRYYKELLSGSQEEYAVYAKSPFQADKRLVLAASDVSSRYSRRGRTQYERIADYIE
                     GVIGGKTGNYMIFFPSYQFLFQVQKVLEERQEQGKLKFEWKAQSSNMSEEEREEFLLS
                     FKEEPKESFAGLCVMGGIFSEGIDLKEERLIGAIIIGTGLPQVNPEQEILKEYFDEQG
                     EGGFDYAYQYPGMNKVMQAAGRVIRTVNDKGVIALLDDRFLLPEYVALFPREWERYTV
                     VNRFNVKQAVDDFWGQFV"
     gene            complement(10083..10958)
                     /locus_tag="ACZPCL_07915"
     CDS             complement(10083..10958)
                     /locus_tag="ACZPCL_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1162425.1"
                     /translation="MDINYELYKVFYHVAVTLSFSEASKQLFISQSAVSQSIKVLEKK
                     LKQPLFIRSTKKVQLTPEGEILLKHIEPAMNLIQKGENQLLEAHTLNGGQLRIGASDT
                     ICRYYLVPYLKQFHKEYPNIHIKVINHTSIECAKALENGQTDFIIANYPNSALAGNLH
                     TRVINEFHDVFVASRQAFDLEGKELTLEELLKYPIMMLDRKSTTSEFLHQIFQKSHLD
                     LVPEIELSSNDLLIDLARIGLGIAFVPDFCIPANDKDLFILELSEKLPARQMVVAFNE
                     SLPVSQAAKQFMDML"
BASE COUNT         3402 a         2078 c         2887 g         2621 t
ORIGIN      
        1 aaccggccaa cttaaatctg tgggccagca ggcctttgga caaagaagag acggactgtg
       61 taagagcagc ttatatcagg gaacatttac ctcagatatg cagctgacag atggattttt
      121 gaaatgcaca gggacataaa ggaggacaac aatggaacga tatacaccgc aggcgaaaga
      181 ggccttaagc ctggcagtag gaatggcaga aagcttaaac cacggttatg tgggaaccga
      241 gcatctgctg atcggccttt tacaggaagg aaccggcgtt gcagccaggg tcttagagga
      301 aaatggggtt gaggagtcaa aggtagtaga attagtcagc cagctgatct ccccaaatac
      361 ttctgtacag atggcagaaa atgcagccta taccccaaga gcccgcaggg taatagaaaa
      421 cagttatagg gaagcagtac gctttaaggc agcacagatc ggtacagaac acattctgat
      481 cgctatttta agagaaggag actgtgtggc aagccgtctt ttaaatacaa tggggatcag
      541 tgtacagaaa ttatatattg atcttctggc agctatgggt gaagatgcgc cttctattaa
      601 agatgagatg cagcgtggaa attccggaaa acggggaagt tccacaccgg cccttgacag
      661 ctacagccgc aatctgaccc agatggcact ggatggaaag ttagatcctg tgatcggcag
      721 ggaacatgag atccagagag tgatccagat cttaagccgc cgtactaaga ataatccgtg
      781 tctgatcgga gagccgggag taggaaagac cgcagtagta gaaggacttg cccagagaat
      841 tgcagcaggg gatgtgccgg ataccattgc ggataagaga gttatgactc tggacctttc
      901 cggtatggtg gcaggttcca agtacagagg cgaatttgaa gagcgcatta agaaagtgat
      961 cgcagaggta gtagaagcaa aagacgtcct tctttttatt gatgagatcc acaccattat
     1021 aggtgctggc ggtgcagagg gtgctctgga tgcatccaat atcttaaagc cgtcccttgc
     1081 aagaggggaa ctgcagctga tcggtgctac taccattaac gaatacagaa aatatataga
     1141 aaaagactct gccttagagc gccggttcca gccggttact gtggacgagc cttcagaaga
     1201 agagtccatt gccattttaa aggggctgag aagccgttat gaggaacatc acagggtaga
     1261 gatcacagat gatgccttag aggcagctgt aaagctttcc agccgctata tcaatgaccg
     1321 tttcctgccg gataaggcca ttgacctgat cgatgaggca gcttccaaag tacgcctgtc
     1381 aaattataca aagccgtcca agatcaagga ttacgaggca cagatcgatg atctggaaga
     1441 agaaaaagag tctgcgattc gtgatgaggc ctatgaaaag gcaggagata ttaagaaaaa
     1501 acaggaaaaa ctgaaagaaa agatccgctt gactctggaa aaatgggaaa aagagaaaga
     1561 aaccagaaaa ctggtagtag gtgaaaatga agtggcagat gtagtagccg gatggaccaa
     1621 gattccggtg aagaaactgg cacaggaaga gtcagagcgc ttaaagaact tagaaggcat
     1681 cctccacgaa agagtcgtag gccaggagga ggctgttacc gctgtatcaa aagccatccg
     1741 aagaggcagg atcggcttaa aagatccaaa gcgtcccatc ggttctttct tattcttagg
     1801 acctaccggt gtgggaaaaa cggaactttc aaaggcactg gcagaagcca tgttcggtac
     1861 agaaagtgcc ctgatcaggg tagatatgtc tgagtacatg gaaaagcaca gtgtttccaa
     1921 gatgatcggt tccccgccag gatatgtagg ctatgaggag ggtggacagc tcagtgaaaa
     1981 agtgcgcaga aatccttaca gcgtgatcct gtttgatgag atcgaaaagg cccatccgga
     2041 tgtatttaat atccttttgc aggtattaga tgacggacac atcacagatg cccagggaag
     2101 aaagatcgac tttaagaata ccattatcat tatgacttcc aatgcaggtg ctgaaaatat
     2161 catcgcaccg aaacgtcttg gcttcggagt tgccacagac gcaaaggcag atcatgaatt
     2221 catgaagggc cgtgtgatgg aagaggtaaa acgcctgttc aagccggagt ttttaaatcg
     2281 tattgacgag atcatcgtat tccatcagct gacgaaagag catatgaagg gaattgcgga
     2341 tattatgctg agaggcattg aaaaacgcag caaagaacag cttggcatta cccttactgt
     2401 aaatgaggcg gcaaaggatc tgctgatcga caagggctat gatgataaat acggtgcaag
     2461 accgctgcgc cgtaccatcc agagcctgtt agaagataag atggcagaag agatcctgga
     2521 cggaaaactg aagaagggtg taaatgtgga agttggctgt gaagaaggaa aacttacctt
     2581 tacagtaaag aagaaagcag cagccagaaa gaaaaaagcg caggcagcag atactgcttc
     2641 aaagcctgag gcaaaggcgt aaaatatact ggttatttta tgaaaattgt tgtataatta
     2701 tagatacccg ttataaatga tctactggga atacggtgaa aacccataaa gtctgacagg
     2761 aatgccggat gaaaaggtac gaccagagaa tttacagata aagagcagga ggataaaaag
     2821 atgccagttg ttgaagaatt gatccgcaca gagcaggatg gcaccatcag ttttggaaat
     2881 tacaagttag gacagaagtc aaagaagtcc gattttgaat accagggaga tatctataag
     2941 gttaaaactt acaacgagat cacaaaatta gagcgcaacg aaatgttcgt atatgagtct
     3001 gttccgggca cagtagcaga acattttaaa gtaacagatg acagtataga gtttgaagta
     3061 gagggcgata aggatgccca gatcacagtt cagctggaaa atgatactga ctacgaagta
     3121 tatgtagacg gagctgctgt gggaagcatg aagaccaaca tgagcggaaa gttaagcgtc
     3181 agcgtagagt tagaagaagg aacttccgtt aaggtaaagg cagtaaagag agcatagaaa
     3241 atatggcaaa agcaaagaca actgcatttt tctgcaaaga atgcggatat gagtcatcca
     3301 aatggctggg acagtgtccc gcctgccggg catggaacac tctggtagag gaaccggtgg
     3361 caggtacagt ttcggttgca ggaaaaggac gtatgtcttc tgatcgagga gcggggagag
     3421 ctgtggctaa acccgctctt ctttcagaaa tacgtctgga acaggaagac cggatctcca
     3481 ctggttttaa ggaactggac cgggtattgg gagacggcat tgtagccggt tccctggtgc
     3541 ttgtaggcgg tgatccgggt attggaaaat caaccctttt gctgcaggta tgccgccatc
     3601 tggcagcagc cggacagaag gtactttata tttccggaga agagtcgtta aaacagatta
     3661 aaatgagggc aaacaggatc ggaacggtca caggggaact gcgcttttta agtgagacga
     3721 atttagagcg cattgaggct gtgatcgatc aggaaaagcc tcagatcgta gtgatcgact
     3781 ccattcagac catgtaccgg gaagagatca cctctgcacc gggcagtgta agccaggtgc
     3841 gtgagtctac cggtgtttta atgcagatcg caaaaagcca gggtattgcc atcttcgtag
     3901 taggtcatgt gaccaaagag ggagtagtgg caggcccaag ggtattggaa catatggtag
     3961 atacagtcct ttactttgaa ggggacagac atgcttctta caggatcctg cgaggcgtaa
     4021 agaaccgttt tggttccacc aatgagatcg gtgtatttga aatgcgggaa gagggtcttg
     4081 tggaagtaga aaatccctct gaatatatgc tggatggacg gcctcaggag gcatcaggag
     4141 cagtggtatc ctgttcctta gagggcacaa gacctatcct tttggaggtt caagcactgg
     4201 tagcggagac aaattttggt atgccaagaa gaacagcggc aggtacggat tacaaccgtg
     4261 taaacctttt gatggcagtg ctggagaaac ggtgccgcta cgaattgtcc cgtctggatg
     4321 cctatgtaaa tatcaccggc ggcatgcgta tgagtgaacc ggctctggac cttgccattg
     4381 tcatggcact gatgtccagc tataaagacc ggcctgtaga tccagatatg ctgatctttg
     4441 gggaagtagg tctttccggc gaagttcgtg gtgtatccca ggcagcccag agagtagcag
     4501 aggcagctaa gttaggattt accacctgtg tgcttccgaa agtaaatatg gataagataa
     4561 agcctgtaga ggggatccgc atgatcgggg tgtcgaatgt gcgggaagcc atcgggttgt
     4621 tataaaaata tagcgtaaaa agaacagcac ctgcctgtgc ctggtgtaaa agccggtgtc
     4681 aggcaggttt atgtttcaat aaaactgtat taaaaaacag aaaaaaataa gcaattactt
     4741 tttttgaaaa aactttgaaa aaaattcaaa atagttgttg acagtgttgg aaacctgtga
     4801 tattataata ctcgttcgag ggaacaaaac aaaaaacaaa agaacatagg ggaatagttc
     4861 aatggtagag cagcggtctc caaaaccgta gatgggggtt cgagcccctc ttcccctgct
     4921 gactatttga gagaatagcc caacccgaag gaagttcaga agtcgtatgg cttttgggct
     4981 ttttcttttg tgttaaaatc tacaaaaaca ggggagatac atacatgaac aaaaaagaat
     5041 gcaacgaaat aaaaaaactt ttcactccgg caaactgtgc catcagccgg atctgcggct
     5101 gctatgtaga ctcagagaaa aataaaaaaa cagaactgaa ggaagcattt ttatcccttc
     5161 cggaagaaga agcctttaaa tattttacca ttttccgcaa tggcctttca ggaaccattg
     5221 gaaaaaatct gatcaatatg gaatttcccc ttcacacaga agaagagggc ggtacccaga
     5281 atttcctttt aaagcttcgg gacagtgaat taaaagacga tgggctgatc gaggaatttt
     5341 atgaccgtgt gatcgccaat tttgactatg cagagaatta ttatattatc ctgatccact
     5401 gtgcatatga cattcctgca aaggctacag acggttctga gatgtttgac gcttctgatt
     5461 atgtgtatga gtttatccag tgtacaatct gccctgtaaa gctttccaag gcaggtctgt
     5521 gctataattc cactaccaat gtgattgaaa acaggatccg tgactggctg gtggaggcac
     5581 ctgtgaccag tttcctgttt cctgcattta atgaccggaa tacagacatc catagccttt
     5641 tactttatgc gaaaaacgga gaacagctgc cggatacact gatcgatgaa gtgttgggct
     5701 gccatatccc tatgtctgcc aaaaaccaga aagagacctt ccagactatc gtagaggaaa
     5761 ctttagggga aaactgcgac tttgtgactg taaaaagcat ccatgaaaat ttaagtgaac
     5821 tggtggaaga gacaaaagat gaacctgcac cgccggtact gaataaaaca cagcttaaac
     5881 agcttctgga aaacaacggg gcagcaccgg aaaaacttca ggaatttgaa agtcgttatg
     5941 cagatgtaga ggacggtcct gaaaccagtt tcgctgtatc aaatgtagtg aatacaaaga
     6001 gctttgagat caaaaccccg gatgtgacca ttaaggtagc ccccgataag acctatctgg
     6061 tggaaaaccg tatgatcgac ggacggccct gccttgtgat cgctattaac gaacatgtgg
     6121 agatcaatgg tatctctgtg cgtcctgtac cactggcaga ccgcaagacg gcaggagaag
     6181 gggcagaaga tacagcacca tggcaggaag aaaatggtac acaggatcct tccggatctg
     6241 atgagatccg tccggtggca acggggatcc gctctgcaga tgaagtataa gaaaaagcga
     6301 atactccccc tggttttgta aaaataagat attatctttc gcaagtttag gatataatct
     6361 ggacaaaata aacaatagtt gttataatga ctatgttggt ctgtgaaaaa taaaacgtac
     6421 cagcaaatgg cgactactcc attggctaga aagaaaattt tacgagcgga aggaagagag
     6481 tacaaatgag tcggacaatt aaagcaggac atgtgtatcg gcagtattcc aaagatggaa
     6541 tgcccatagc agattataaa agttcaagac tggcccacga ggcaacaggt gtttctttag
     6601 gctccattgc cagagcagcc catggcgagc gtaagacagg aggaggctat ctgtggcgtg
     6661 tagttccgga agacctgccc tgggaaccga tccaggtgga cttaagcagc aagatcggat
     6721 accatgataa gagaccgctg atccagctga cattagacgg acaggtagta gcagaatatc
     6781 tttctattgc tcatgccagc cgtgctttaa atatcagccg cagaagtatt tcctgtgcga
     6841 tgacaggagc tcagaaaaca gccggcggtt ataaatgggt ggaaaaagaa actcccgaaa
     6901 caaaagaaaa agagtaacta ttcaggtgga tttccaggat atgggaagcc acctttcttt
     6961 tatgcataaa atcagataca atgtaaaaat aatttacagc gatttatata aatatttacg
     7021 aaaaagggca taatctgaaa agaggaataa aatttgacat tgaaaaataa tagtgatata
     7081 tatattccaa cgttcaggaa tgactggacg gacagcactt taaccaggat gatctgcctg
     7141 taaaaaacag gtggaaatct taaaaaggag tcttgaatta tgaaagaaaa gaaaatcatg
     7201 ctgccatctg tagaagaagc aaaacatttt gtagcagagg caacgaaatg tgactttgat
     7261 attgacgtat tctataaccg tgtgaccatt gatgccaagt cgatcttagg tgttttaagc
     7321 ctggatctgg cgcgggtact gaccgtacag atgaatggtg ataatgcaga atttgaagaa
     7381 tacctggata ccctggcacc tgtatctacc actgctgctt aaagtgggat gacaggatca
     7441 tggcaatcgc agaataagaa tcagaaataa gaaaaagagc gggaacgaac atcagtttgc
     7501 attcctgctc tttttgtgtt atcctaaagt catgaatttt aggagtacag atggagggaa
     7561 acattggaag gcagtttgat gacagaagca cagtctaaga tccgcatctc tgtccgcaat
     7621 ttagtagagt ttgtcctgcg gtccggggat ctggataacc gcaggagcca gggcggaaaa
     7681 aaagaggcta tgcaggcggg gagcaggctg cacagaaaga tccagaaacg catgggagcg
     7741 gattaccgtt cagaagttgc tttgaaacat caggtgcgtg aagatgaatt cagtatcctt
     7801 ttagaaggca gggcagacgg gatcattaca gaaatgtccg ggatcgtgat cgatgagatc
     7861 aaatgtattt acatggacgt agagcgtttg gaagagccgg atccggttca tcttgcacag
     7921 gccctgtgtt atggatatat gtatgccagt gagcaggatc tggacagtgt ggggatccag
     7981 cttacctatt gtaatataga aacagaacag atccgccgtt tccgccaggc aaaaacaagg
     8041 gaagaactgg aagaatggtt tcagggtctg atccatgaat atgtaaagtg ggccagatac
     8101 ctttaccatc acagcatccg cagaacagaa tgtttaaaag aactgcagtt tccttatgca
     8161 tacagggaag gtcagaaaga actggcggtt tctgtataca gaagtattgc cagagggaga
     8221 aatctttaca tacaggcgcc tacagggatc ggaaaaacct tatcctgtgt ctttccaagc
     8281 ctgaaagcta taggagaagg ctatggggaa aagctgtttt atctcacagc caagactatt
     8341 accagaagcg tggcagaaga gacgtttgaa ttgttaaggg agcgggaaaa cctgtatttc
     8401 agtactgtga ccattactgc aaaagaaaag ctgtgtatcc tggaaaaacc ggactgcaat
     8461 ccagttgcgt gcccaagggc aaagggacat tttgaccggg tcaatgacgc tgtttatgag
     8521 atcatacagg aagagcaggg gattaccagg gaaacgatcc ttgcatatgc agagaaatac
     8581 caggtctgcc cctttgaact ctgtctggac atcagcagct gggtagacgg catcatctgt
     8641 gattataatt atgtatttga tcccaatgtg cgcctgaaac gctattttgc agaaggagaa
     8701 gagaggggaa attatatttt ccttgtagat gaggcccaca acctggtttc aagagccagg
     8761 gagatgtaca gtgctgttct tataaaggaa gagctgcttg ctgcaaaaag gatcttaaaa
     8821 gggatccccg aggcaggaaa agtcctgaag ctgttagaac ggtgtaatcg caggatgctg
     8881 gatctaaaac gcctggggga gacagaaccc ttttctgatg aagcagacat ggaactggcg
     8941 tttgaagggg gagcacggat ggttctgggg gaacattacc gcatgcattc ggatgtaaaa
     9001 cttctttccc tggagttaat ggggctgttt ggggagatgg aagtattttt aaatgaaaac
     9061 agcgaatttg atgacaggga gacagtcctt gatctgtatt ttaagatccg tgatttcctt
     9121 tatgtaagtg accggctgga tgaaaattac aggatataca gccgcttact ggcggatggc
     9181 tcttttatgg taaaactgat gtgtgtcaat ccttccagct gcctgcggga atgcctggac
     9241 cggggcgtga gtacagtgtt tttttctgca acgctgctgc cgatccgtta ttataaggaa
     9301 cttttaagcg gaagccagga agaatatgcc gtgtatgcaa aatctccttt tcaggcagat
     9361 aaacggctgg tacttgcggc ttctgatgta agcagccgtt acagcagacg gggcaggacc
     9421 cagtatgagc ggatcgctga ctatatagaa ggggtcatag gaggaaagac aggaaattac
     9481 atgatctttt tcccatccta tcagtttctg tttcaggtac agaaggttct tgaggaacgg
     9541 caggaacagg gaaagttaaa atttgaatgg aaagcccagt catcgaatat gtcagaagaa
     9601 gaaagggaag aatttctttt aagttttaag gaagaaccaa aggagtcatt tgctggtctt
     9661 tgtgtaatgg gcggtatttt ttcagaaggg atcgatctga aagaagagag actgatcgga
     9721 gcgatcatca taggaacagg acttccacag gtaaatccgg aacaggagat cttaaaggaa
     9781 tattttgacg aacagggaga aggtggcttt gattacgcct atcagtatcc ggggatgaat
     9841 aaagtaatgc aggcagcagg ccgggtcatc cgcactgtaa atgataaagg ggtgattgcc
     9901 cttttggatg accgtttcct gctgcctgaa tatgtggcat tatttccaag ggaatgggaa
     9961 cgttatacgg ttgtgaaccg ctttaatgta aaacaggcgg tagatgattt ctggggacag
    10021 tttgtgtagt ggctgcagca gtctgacgtg tggtcaaaga acttctgtga aaggttgcca
    10081 ttttacaaca tatccataaa ctgcttggca gcctgggata caggaaggct ttcattgaaa
    10141 gcaaccacca tctgacgggc aggaagtttt tctgaaagtt ccaggataaa cagatcctta
    10201 tcattagccg ggatacagaa atccggaaca aaagcaatcc cgagaccgat gcgggccagg
    10261 tcgatcagca ggtcattact ggaaagctcg atctccggta ccaggtccaa atgacttttc
    10321 tggaaaatct ggtgcaggaa ttcactggtg gtacttttac ggtctaacat catgatcggg
    10381 tattttaaca gctcttctaa ggtcagttcc tttccttcca agtcaaaggc ctgacggctg
    10441 gctacaaata catcatggaa ttcattgatg acccgggtgt gcaggttgcc tgcgagagca
    10501 gagttaggat aattggcaat gataaaatca gtctgaccat tttccagggc tttggcacat
    10561 tcaatggaag tgtggttgat cactttgata tgaatgttgg ggtattcttt gtggaactgt
    10621 tttaagtagg gtaccagata gtagcggcag atggtgtcgc tggcgccaat acgcagctgg
    10681 cctccgttta aggtatgggc ctctaacagc tggttttccc ccttctggat caggttcatg
    10741 gctggttcaa tatgttttaa taaaatttcg ccctctggtg tcagctgtac ttttttggta
    10801 ctgcggatga aaagcggctg tttcagcttc ttttcaagga ccttaatgga ctggcttaca
    10861 gcagactggg aaataaaaag ctgtttggaa gcttccgaga aactaagtgt aacggctacg
    10921 tgataaaata ccttgtataa ttcataatta atatccatta ttaagtcctc cttataatta
    10981 cctatatt
//
LOCUS       DBMDUA010000087        10973 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2964_length_10973_cov_2.418506, whole genome shotgun sequence.
ACCESSION   DBMDUA010000087 DBMDUA010000000
VERSION     DBMDUA010000087.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10973)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10973)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10973
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2964_length_10973_cov_2.418506"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..3348)
                     /locus_tag="ACZPCL_07920"
     CDS             complement(<1..3348)
                     /locus_tag="ACZPCL_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HIR1162426.1"
                     /translation="MNTKIWKRMMAGLLAGVLIFTGIPFSASAGEPPVQMIIRGDAFQ
                     NRQINIFGHSHRSITCYYVNPVEGHVPAFCLQPGKKLPNHTQASWERYSASPETSVPV
                     IGSFDRYLPMTLAYEWMVSGNYYDKTRYAMVQTYFWGCLAGFEQDWDIQEETMKKLEW
                     AIRDGRVLSLFHEMRSVVEKGLEEYESNSGSSLPAWNGRKENMILKDGHYELTLDLSS
                     CPQLKNANWQFPDKNWSFTQGPGENEITFLYTGEEPSGRISAGNVEGLDDRYYAYIFY
                     PGESYQMQMGWLDMQRPQAEVWFETGKGSVQGGQMQPLERFRHREVFEADYKIDLEKY
                     CAETGQPLAGADFQVWESFDHSQINEHGYEEGEPDGTTGQVYSNCMSPEPEEEWLCDV
                     ITTDEEGKAFHKDIRNYNYSKTYCMGHPAPEWIECDHEGDGGDGEDGEGEEEECNCEE
                     ENERLREQWLEEQELCEASSDFHVSNEDEEDHEQSTEAMEAMLEDRDETYENFIALEY
                     GYTLTEKKARTGYMVHGTHREDFEIEKITVASAQAQRNTAGKEGTGREDPQSEVYLGE
                     NTQLQFHPAPQKKEKKNGYAYETGAQEERSAEELRTIHEVKLTEDIYEEEEDDEDEIW
                     DDRATESNAKISRTTANTATESKATASDAAEDNAVVAAATASNAKKDLETTEQYIYTR
                     EEISLEPISLWESLRENVWNSVKAIFFSSEDDDDFEGITAFLPDFQDDDLGEMDVSCF
                     GDPSNSLYCFKVWDHRTEGRIYINKRDLHLYEKDQEESYGRTQGDGTLEGAVYGLFAA
                     ADIVHPDGRSGVIYQKDDLTAVAATDQDGNASFFAYTEVPHTVVKEDGTIEAPENFLG
                     PETLYNGSTINSSDHGFGSYTYPDRKEENGNSWIGRPLLMGSYYVKELSRSEGYELSV
                     TGKNLVETNRGSGEENVVRKAGQVQVSAGLSEHNSMEADGSWNDFTVEAFGTEKGYEI
                     TVSGYPENTKFYRLSSQEQRENLKQITGMEKIPKMDHLGNPVYEKAKGGELKRDLNGN
                     PVLKADAGEDEKVPASETVPYHFRTAPYIKGEAKPEDLSKWDEDLDPDYVMEQAQAML
                     MQLGYKEADGEGAPWVSINLDSMNGKAV"
     gene            complement(3680..5308)
                     /locus_tag="ACZPCL_07925"
     CDS             complement(3680..5308)
                     /locus_tag="ACZPCL_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HIR1162427.1"
                     /translation="MGKVSLGNGKRWTMNQILLLTGIVIVICILSGRLIDKLGVPSLL
                     AFILLGMCFGVDGIFKISFDDYQFSELICSVSLIFIMFYGGFGTNIKEAGPVIPRAVL
                     LSTAGVLFTAGITGLFIHLALKLPLLESFLVASVIGSTDAASVFNILRSKNLNLKENT
                     ASLLEMESGSNDPVSYMLTVILTAVLTGQSVNVGSMLVFQILFGIGCGVFFGKVTAFI
                     LNKVEFDIAQGKTILVFATAILAYTIPSMIGGNGYLSVYLCGILLGNSYLPHKRDMVL
                     FFDVLTGVAQMMIFFLLGLLVTPSQLPQVIIPSFVIMIFLTLVGRPVSVAALLAGFRA
                     SIGQMGLVSWAGLRGVASIVFAIYVVLRQVPLGYNLFNLVFCIVLLSISIQGTLLPWI
                     SGKLQMIDENADVRRTFNDYQEEESISFVKFHLFSQHPYVGKCLKEITLPPKLLVVVL
                     IRGKEVLVPNGDTCLQAGDLMIAAAEEFEDRKNMTLREITVDKNHKWKGRSLSRIPMQ
                     AGTLIVMIRRGKETVIPTGNTVVYEGDVLVQAKF"
     gene            complement(5464..6699)
                     /locus_tag="ACZPCL_07930"
     CDS             complement(5464..6699)
                     /locus_tag="ACZPCL_07930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1162428.1"
                     /translation="MKKRRWICMFLLLQVLWCSMTGMAVYGMEPDIPVSAVVSGQAQA
                     VSIQAPSAVLMEASTGKLLFEKDADEKRSPASVTKVMTLLLIFDALKAGKIQMTDQVT
                     TSAYAKSMGGSQVFLEEGEVQTVETLIKCIVIASGNDASVAMAEYISGTEEAFVEEMN
                     KRAEGLGMTNTHFSDCCGLTEDPDHLTTARDIALMTRELINCYPEIHNYSTIWMENIT
                     HVTKQGTKEFGLANTNKLLKMAADFQVTGLKTGSTSRAKYCLSATAQKDGIRLIAVIM
                     AAPDYKARFSDARNLLSYGYARCRLYEDKEKMPLPEMKVAGGVKENVLLEAEGTFTWL
                     STEGEDLGKIEKKLELAEQMEAPLARGERAGMIAYYMDGEKLGELPVVTAEAVEKAGY
                     IDQLQKLFGKWAGFRNKAA"
     gene            complement(6882..7322)
                     /locus_tag="ACZPCL_07935"
     CDS             complement(6882..7322)
                     /locus_tag="ACZPCL_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR1162429.1"
                     /translation="MGEMVKDPNCAYCVEGELLAKFGYPCCEMKTGKLYVFKEQSKRG
                     RVILAHKKHVSELIELTDEERNDYFADVAKVARAIHKVFNPDKVNYGAYGDTGHHLHF
                     HIVPKYKGGEEWGSTFEMNSGKTILTDAEYEEMAEAIRQALKEV"
     gene            7511..9004
                     /locus_tag="ACZPCL_07940"
     CDS             7511..9004
                     /locus_tag="ACZPCL_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HIR1162430.1"
                     /translation="MSNLNFRKRRNRRGFFFHLSRHLPTFIVLFVIALVLGGGIFAFS
                     RFVLKKQPGDRPVFATASEATKSNGTPSASAEADSDDPIGQLIDQADRLAKGYDYDKA
                     MELLSQDPSIQSDPRISEALASFQSQKDSLVAADVKNVTHVFFHSLIMDTSKAFDGDS
                     RMEGYNSVMTTKDEFLKILDALYNDGYVLVRIHDIAYETTDENGNPAFTWGTIMLPEG
                     KKPIVMSQDDVCYYPYMDGDGFASKIVVGEDGRPTCEMKMDDGSISTGSYDLIPLLND
                     FIDEHPDFSYKGAKAIIALTGYEGILGYRTASSYSETPDYESEKEQAARVAQCLRDDG
                     WELASHSWGHLWMGVSDDPENPYKISDERFYTDTDKWKNEVESLIGPTDIYIYPNGND
                     VADWHPYTEENYRYQYLASKGFRYFCNVDASKPAWIQKGPDYLRMARRNLDGYRLYED
                     MIQEDPAKKRLSDLFDASQIFDPSRPTPVTWNYGHTQNETPAPEPEQ"
     gene            9045..9836
                     /locus_tag="ACZPCL_07945"
     CDS             9045..9836
                     /locus_tag="ACZPCL_07945"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1162431.1"
                     /translation="MKPKLQVSRVSFSYHSKNRETLALSDISFNVDTGEFIAIVGPSG
                     CGKSTLLSLLCGLNMPEAGAIFLDGELLEKNPKNATGIGYMFQKDHLFEWRDIFSNVS
                     LGLEIQKKLNTETKQELSDLLSDYGLAGFEHAKPSALSGGMRQRAALIRTLALKPDLL
                     LLDEPFSALDHQTRLMVCDDISSILRKNGKTAILVTHDLSEAISFADRIIVLTPRPGR
                     IRTVLYLSFPDDCNSPLKRRNCPEFSHYFNTLWKELIKDDQTAGI"
     gene            9814..10596
                     /locus_tag="ACZPCL_07950"
     CDS             9814..10596
                     /locus_tag="ACZPCL_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1162432.1"
                     /translation="MTRQQEYEARLLLQTRQIRICRIMILILFLSLWELTASLGLINR
                     FIFSSPSQILFCLWNMLLDKSLFLHTGVTLTETLLSFLLCTLISLLTALFLWSSPFAA
                     QIFEPFLVLLNSLPKSALAPLLLVWLGNRMRTVIVAAVSIAVFGSVMTLYTGFNQMDP
                     DKIRLIYALGGAKKEVLTKVLLPGSLPLLMSTMKVNIGLCLVGVIIGEFLSAKAGLGY
                     LITYASQTFEMTKLMSALIMLCLLSCVLYQAVGMLEKQFLKQ"
     assembly_gap    10706..10715
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10716..>10973)
                     /locus_tag="ACZPCL_07955"
     CDS             complement(<10716..>10973)
                     /locus_tag="ACZPCL_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HIR1162433.1"
                     /translation="AEELKATLANNDYTSVAKTVLEGVGGPENVASIDNCITRLRLEV
                     KDYTKVNEKVIKSAGVAGVMRPSKTSVQVIIGTQVQFVADE"
BASE COUNT         2785 a         2782 c         2095 g         3301 t
ORIGIN      
        1 tacggccttt ccattcatag aatccagatt tatagaaacc catggtgccc cttccccgtc
       61 agcttcttta tagcctaact gcataagcat tgcctgtgcc tgttccatca cataatcagg
      121 atccaggtct tcatcccatt tagacagatc ttccggtttt gcttctcctt ttatataagg
      181 ggctgtccgg aaatgatagg gcacagtctc agatgcaggt actttttcgt cttcccccgc
      241 atctgctttt aaaacaggat tgccattaag atcccgtttt aattctccgc cttttgcctt
      301 ttcatataca ggatttccca aatgatccat tttgggaatt ttttccattc ctgtgatctg
      361 ttttaaattt tccctctgct cctgtgaact taaacggtaa aactttgtat tttccggata
      421 accggaaact gtgatctcat accctttttc tgttccaaat gcttctactg taaaatcatt
      481 ccaggaaccg tctgcttcca tactgttatg ctcagacaga cctgcgctta cctgtacctg
      541 ccctgccttt cgcactacat tttcttctcc tgacccccgg ttcgtctcca ccaggttctt
      601 tcctgttaca gacagttcat atccctctga ccggcttagt tctttcacat aataacttcc
      661 catcagcagc ggtcttccga tccaggaatt tccattttct tcctttctgt ccggataggt
      721 atagcttcca aatccatgat cagaagaatt aatggtactg ccgttatata aggtttccgg
      781 ccccaggaaa ttttccggtg cctcaatggt cccatcttct tttactactg tatgcgggac
      841 ctccgtgtat gcaaaaaatg aggcattacc atcctgatct gttgctgcca cggctgtcag
      901 atcatctttc tggtagatca caccggatct tccatccgga tgtacaatgt ctgcggctgc
      961 aaaaaggcca tatacagcac cttctaaagt tccgtctccc tgggtccggc catagctttc
     1021 ttcctggtcc ttttcataaa gatgcagatc ccgtttattt atatagatcc ttccttctgt
     1081 gcggtggtcc cagactttaa aacagtaaag ggaattcgat ggatcaccaa aacaggaaac
     1141 atccatctct cccagatcat catcctgaaa atcaggaaga aatgctgtta tcccctcaaa
     1201 atcatcatcg tcttctgagg aaaaaaagat tgcttttaca gaattccata cattttcccg
     1261 taaagactcc cataaagaaa tcggttccag ggaaatttct tctcttgtat aaatatattg
     1321 ttctgtcgtt tccagatcct tctttgcgtt cgatgcagtg gcagcagcta caactgcgtt
     1381 atcttctgct gcatcacttg cggttgcttt gctttctgtt gccgtatttg ctgttgtcct
     1441 gcttatcttt gcattacttt ccgttgcccg gtcatcccag atctcatcct cgtcatcttc
     1501 ctcttcttca taaatatctt cggtcagctt cacttcatgg atcgttctaa gttcctctgc
     1561 tgatctttct tcctgcgctc cggtttcata agcatatccg tttttctttt cttttttttg
     1621 tggagcggga tgaaactgca gctgcgtatt ttcccccaga tacacctctg attgcggatc
     1681 ttctcttcct gtaccttcct ttcctgctgt atttctctgt gcctgggcgg aagccactgt
     1741 tattttctcg atttcaaaat cttccctgtg tgtcccgtga accatatagc cggttctggc
     1801 ctttttctct gttaatgtat agccatactc cagggcaata aaattttcat aggtttcatc
     1861 ccggtcctct aacatggctt ccattgcttc tgtactctgt tcatggtcct cttcatcctc
     1921 attggataca tggaaatcgc tggaagcttc acacagttcc tgttcctcaa gccactgctc
     1981 cctgagacgt tcattttctt cttcacagtt acattcttcc tcttcgcctt ccccgtcttc
     2041 tccatcaccg ccatctcctt cgtgatcaca ttcgatccat tccggcgccg gatggcccat
     2101 acagtaggtt ttgctgtaat tgtaattgcg gatatcttta tgaaaagcct ttccttcttc
     2161 gtcggtggtg atcacgtcac acagccattc ttcttccggt tccggggaca tacagttact
     2221 gtaaacctga cctgtcgttc cgtccggctc cccttcttca tagccatgtt cattgatctg
     2281 ggaatggtca aaactttccc atacctgaaa atccgctcct gcaagaggct gtcctgtttc
     2341 tgcacaatat ttttccagat ctattttgta atctgcctca aaaacttccc tgtggcggaa
     2401 ccgttctaaa ggctgcatct gaccgccctg gacactcccc tttcctgttt caaaccatac
     2461 ttctgcctgg ggacgctgca tatccagcca gcccatctgc atctgataag actctcccgg
     2521 ataaaagata taggcataat accggtcatc caagccttcc acgtttcctg cgctgatccg
     2581 tccggaaggt tcttctcctg tataaagaaa tgtgatctca ttttcccctg gtccctgtgt
     2641 aaaactccag tttttatccg ggaactgcca gtttgcgttt ttaagctggg gacaggaact
     2701 aagatctaag gtcagctcat aatgaccgtc ttttaagatc atattttcct tacggccatt
     2761 ccaggcagga agactacttc cgctattcga ctcatattct tccaggccct tttccactac
     2821 cgacctcatt tcatgaaaca gggagagaac ccttccgtct cttatagccc attccagttt
     2881 tttcatggtc tcttcctgga tatcccagtc ctgctcaaaa cctgccagac agccccagaa
     2941 ataagtctgc accatggcat accgggtctt atcataataa tttcctgaaa ccatccactc
     3001 ataagccagt gtcataggca gataccggtc aaaacttcct atgaccggta cggaggtttc
     3061 cggtgacgca ctgtacctct cccaggatgc ctgggtgtga ttgggaagtt tctttcccgg
     3121 ctgcaggcaa aatgccggta catgtccttc caccggattt acataatagc aggtgatcga
     3181 ccggtgactg tggccgaaaa tgttgatctg cctgttctga aaggcatctc ccctgatgat
     3241 catctgcaca ggcggttcac ctgcagaggc agaaaacggg attcctgtaa aaataaggac
     3301 gcctgccaat aagccagcca tcattcgttt ccatattttc gtattcaatt gtttttcccc
     3361 ctttggaatg tgttttgatg tgtttgatct gatttgtcct gatgtgatgt gtttagattt
     3421 gatgtttgat cttttccatg atctgaattt tagaaatgtt gcaaaatccc ctgatatttt
     3481 aatccaaact ttcccctcct ttttcatttt ttcacttttt tacaattttt gtgaaactgc
     3541 ggtctgaaac gaccaaaaat aaatagaata aacagatctg acagggagat ctccccgccg
     3601 atggtactag gataacataa gaaaaagagc aagtcaatga gacctgccct tttcatagca
     3661 ctttgatgat cataatactt taaaattttg cctgtaccag cacatcccct tcatacacca
     3721 ctgtatttcc tgtagggatc acagtttctt ttccccttcg tatcataacg atcaaagttc
     3781 ccgcctgcat agggatacgg cttaaagatc ttcctttcca tttatgattt ttatccacgg
     3841 ttatttcgcg gagcgtcata tttttccggt cttcaaattc ctctgccgct gcgatcatca
     3901 gatctcctgc ctgcaaacag gtatctccat tgggaactaa cacttctttt ccccggatca
     3961 gcaccacaac caacagttta ggcggcaatg tgatctcttt taaacatttt cccacataag
     4021 gatgctggct aaaaagatga aactttacaa agctgatgct ctcttcttcc tgatagtcat
     4081 taaatgtacg gcgcacatct gcattttcat cgatcatctg caactttcct gagatccatg
     4141 gaagcagggt tccctggata gaaatggaca acagtacaat gcaaaatacc aggttaaaca
     4201 ggttatatcc taaagggacc tggcgcagca ctacatagat tgcaaaaaca atggaagcaa
     4261 caccccgaag tcccgcccag gacaccagtc ccatctgccc gatagatgcc cgaaaaccag
     4321 ctaaaagtgc ggctacagac acaggacggc caaccagggt aagaaagatc atgatcacaa
     4381 aagaaggaat gatcacctgt ggcagctggc ttggagttac taaaagtcct aacaggaaaa
     4441 agatcatcat ctgtgccaca cctgtaagca catcaaaaaa caacaccata tcccgcttgt
     4501 gaggaaggta ggaatttcct aacaggatcc cgcaaagata tacacttaaa tatccatttc
     4561 cgccgatcat ggaaggaatt gtatatgcca gtatagccgt tgcaaatacc agaatggttt
     4621 ttccctgggc aatatcaaat tccactttat ttaaaataaa cgctgtgact tttccaaaaa
     4681 acacaccaca gccaatgccg aacaggatct ggaaaaccag catggaacct acatttacag
     4741 actgtcctgt gaggactgct gtaaggatca ccgtcagcat ataggaaaca gggtcattgg
     4801 aaccggactc catttccagt aaagaggctg tattttcttt cagattcaga ttttttgacc
     4861 tgaggatatt aaatacagaa gctgcatctg tagaaccgat cacagatgcc actaaaaaac
     4921 tttcaagaag aggaagtttt aacgccagat gaataaacag tcctgtgatc ccggctgtaa
     4981 ataaaacacc ggctgttgaa agcagaacag ccctggggat caccggcccc gcttctttaa
     5041 tattcgttcc gaaaccacca taaaacatga tgaaaataag actgacggaa cagatcagtt
     5101 cactgaactg ataatcatca aaggatattt taaaaatacc atctacccca aagcacattc
     5161 ccagaaggat aaatgcaagt aatgacggaa ctcccagttt atcgatcagc cgtcctgaca
     5221 gaatacagat cacaatgacg atccctgtaa gcagtaagat ctggttcatg gtccacctct
     5281 ttccgtttcc cagactcact tttcccacac ctgcatacgt ccctgtcttc tgacgtgacc
     5341 gttcttgcct gggtagatta tattttgacc ctggtatcat gataatatga aaggtattat
     5401 aatggtataa aaccgctccc gtcaaaggtt ttccctgtca tttcctgcat tttaacagct
     5461 tctttatgct gccttattgc gaaaacccgc ccattttcca aataatttct gcagctggtc
     5521 tatgtatccg gccttttcta ctgcttctgc cgtcacaacc ggaagttctc ccagcttttc
     5581 cccgtccata taataagcga tcatgccggc acgttccccc ctggctaagg gtgcttccat
     5641 ctgctctgcc agttccagtt ttttctcgat ctttcccaga tcttccccct ctgtgctcaa
     5701 ccaggtaaag gttccctctg cctccagcag tacgttttct ttcactcctc ctgcaacttt
     5761 catctcagga agaggcattt tttctttgtc ctcataaagc ctgcatctgg cataaccata
     5821 gcttaaaaga tttctggcat cagaaaaacg tgctttataa tccggcgctg ccatgatcac
     5881 ggcgatcagg cggatcccgt ctttttgtgc agtagcggaa agacagtatt ttgccctgga
     5941 ggtggaacct gtttttaaac cggttacctg gaaatctgct gccattttca gaagcttatt
     6001 tgtatttgca aggccaaatt ccttggttcc ctgcttcgtc acatgagtga tattttccat
     6061 ccagatggtg gaataattat ggatctcagg atagcagttg atcagttccc tggtcataag
     6121 ggcaatgtcc ctggctgtgg tcagatgatc cggatcttct gtcaggccac agcagtccga
     6181 aaaatgggta ttggtcattc ccagcccctc tgcccgtttg ttcatttctt ctacaaaagc
     6241 ctcttctgtc ccagaaatat attctgccat agcaacagaa gcatcatttc cggaagcgat
     6301 cacaatgcat ttgatcagtg tttctacggt ctgtacttct ccttcttcta aaaacacctg
     6361 ggaaccgccc atggactttg cgtatgcact ggtggtcacc tggtccgtca tctggatctt
     6421 gcctgctttt aaagcatcaa agatcagaag cagtgtcatt accttggtca cgctggcagg
     6481 actgcgcttt tcgtctgcat ctttctcaaa caacagcttt cctgtggatg cttccattaa
     6541 aacagcggaa ggtgcctgta tggaaactgc ctgcgcctgc cctgaaacaa ccgctgatac
     6601 cggaatatcc ggctccatcc catatactgc catcccagtc atactgcacc acagaacctg
     6661 aagcagcaga aacatacata tccatcttct ttttttcata tcttcccctt ttacagatct
     6721 tctgcaattt atttctttaa cataaatatg aaaaaagaag cacctgtata acctgacggt
     6781 ttctaaataa aaagagaact gaagccctgg atgatctctc accgtttcgg ccgcagttct
     6841 catttttatg aaacaggatc ctgtttcaat tttattctgt tttagacttc ctttaatgcc
     6901 tggcggatcg cttcagccat ttcctcatat tctgcatctg taagaatggt cttgccgctg
     6961 ttcatctcga atgtactgcc ccattcttct ccacctttgt attttggaac aatgtggaaa
     7021 tgtaaatgat gtccggtatc accgtatgca ccatagttta ccttatcagg gttaaatact
     7081 ttgtggatgg ctctggcaac ttttgcaaca tctgcaaaat agtcatttct ctcttcatca
     7141 gtcagttcga tcagttcgct gacatgtttc ttgtgagcta agatcactct tcctctcttg
     7201 ctctgctctt tgaatacata cagcttacct gtcttcatct cacagcaagg ataaccaaac
     7261 ttagctaaca gttctccttc cacacaatat gcgcaatttg gatctttaac catttctccc
     7321 atttttgaga cctcacattc tgttgaattt tacttcattt cttagaatac cacggagatg
     7381 ttaaaaaatc aacaaaaagg actatatgtt atatccaaca ggcgtatttt atgctatact
     7441 gtatagactt aaaggaattt caacaatacc acaagcaata tgattatcag aaatatgacc
     7501 aggagatttt atgagtaatt taaattttag aaagagacgg aaccgccggg gatttttctt
     7561 ccatttgtcc cgccaccttc ctacttttat agttcttttt gtgatcgccc ttgttctcgg
     7621 gggcggtatt tttgcctttt cacgctttgt tttaaaaaag cagcctggag accgtccggt
     7681 ttttgccact gcttctgaag ctacgaaaag caacgggacc ccgtctgcat cagcagaagc
     7741 tgattccgac gatcccattg ggcaattgat cgatcaggca gaccgtctgg caaaaggtta
     7801 tgactacgac aaagcaatgg aacttctgtc ccaggatcct tccattcagt ccgatccacg
     7861 gatcagcgaa gcccttgctt ccttccagtc ccaaaaggac tctctggtag ctgcagatgt
     7921 aaaaaatgtg acccatgtat ttttccactc tctgatcatg gacacttcca aagcatttga
     7981 cggtgactcc cgtatggaag gatataattc cgttatgact accaaggatg agttcttaaa
     8041 aatattagat gccctttaca atgacggcta cgttttagtg cgcatccacg atattgccta
     8101 cgaaacaaca gatgaaaacg gaaatcctgc tttcacctgg ggaacgatca tgctgccgga
     8161 aggcaaaaag cccattgtta tgtcccagga tgacgtatgc tactatccat atatggatgg
     8221 agacggattt gcaagcaaga tcgtagtcgg cgaggacgga cggcctacct gcgaaatgaa
     8281 aatggatgac ggcagcattt ccacaggttc ttatgacctg atccctcttt taaacgactt
     8341 tattgatgag cacccggatt tctcctacaa aggagccaag gctattatcg cacttaccgg
     8401 ttatgaaggg atcttaggat accgcactgc ttcttcctac agcgaaaccc cagattatga
     8461 aagcgagaaa gagcaggctg ccagggtagc ccagtgctta cgggatgacg gctgggaact
     8521 ggcaagtcac agctggggac acctgtggat gggcgtttcc gacgatccgg aaaatccata
     8581 caaaatcagt gatgaacgtt tctacacaga tacggataaa tggaaaaatg aggtggagtc
     8641 cctgatcgga ccgaccgata tttacatcta tcctaatgga aacgatgttg ctgactggca
     8701 cccgtataca gaggaaaact atcgttacca gtaccttgct tccaaaggtt tccgctactt
     8761 ctgtaatgta gatgccagca agcctgcatg gatccagaag ggacctgatt atctgcgaat
     8821 ggccagaaga aacctggatg gttaccgcct gtacgaagat atgatccagg aagatcctgc
     8881 taaaaagcgt ttaagcgatc tgtttgacgc ttcccagatc tttgatcctt cccgtcctac
     8941 tcctgttaca tggaattacg gacatacaca aaatgaaaca cctgcaccag aaccagaaca
     9001 gtaacgtata ttaaagcaag aacgtttctt ttcggagcct ttctatgaaa cccaaattgc
     9061 aggtttccag ggtcagcttt tcctaccata gtaaaaacag ggaaacgctg gccctttctg
     9121 atatttcttt taacgtagat accggagaat ttatagccat tgtaggtcct tccggctgcg
     9181 gaaaatccac tcttttatct ctcttatgcg gactgaacat gccggaagca ggcgccattt
     9241 ttctggatgg ggaacttttg gaaaaaaatc ccaaaaatgc caccgggatc ggctatatgt
     9301 tccagaagga ccaccttttt gaatggcggg atattttttc caatgtttcc cttggacttg
     9361 agatccagaa aaaattgaat actgaaacaa aacaggaact ttctgacctg ctctctgact
     9421 atggacttgc cggttttgaa cacgcaaagc cctctgccct ttccggaggt atgcgtcaga
     9481 gagccgctct catacgtaca ctggcactga aaccggatct gcttttatta gatgagccat
     9541 tttctgccct tgatcatcag acaaggctta tggtatgtga tgatatcagc tctattttaa
     9601 ggaaaaatgg aaagactgct attttagtga cccacgacct gtcagaagct atcagttttg
     9661 ctgaccgtat tatcgtcctc actcccagac ccggccgtat ccgcacagtc ctttaccttt
     9721 cttttcctga tgactgtaac agccccctaa aaaggcgcaa ctgtccggaa ttttcccatt
     9781 actttaatac tttatggaag gagctgataa aagatgacca gacagcagga atatgaagcc
     9841 cgcctccttt tgcagacccg ccagatccgg atctgccgta taatgattct gatcctgttt
     9901 ttatccctct gggagctgac tgcctctctt ggcctgatca accgctttat cttcagttcc
     9961 ccttcccaga tccttttctg tctgtggaac atgcttcttg ataagagcct ttttctccat
    10021 acaggggtaa ccttaacaga aaccctttta agctttcttt tatgtaccct tatcagcctt
    10081 ttaactgccc tttttctctg gtccagtcct tttgcagccc agatttttga accgtttctg
    10141 gtacttttaa acagtcttcc aaaatcggct ctcgcccctc ttcttttagt atggcttgga
    10201 aaccgtatgc gcacagttat cgtagccgct gtgtccattg ctgtttttgg ctctgttatg
    10261 actctgtata caggctttaa ccagatggat ccagacaaaa tacgtttgat ctatgcctta
    10321 ggcggcgcaa aaaaagaggt acttaccaag gttcttcttc ctggaagcct tcctctgtta
    10381 atgagcacta tgaaagtaaa tatcggtctc tgtctggtcg gtgtgatcat cggggaattt
    10441 ttaagcgcca aagcaggact tggctacctc atcacctatg cttcccagac ctttgagatg
    10501 acaaaactga tgagcgccct gatcatgctc tgcctgttgt cctgtgtgtt gtatcaggca
    10561 gtagggatgc ttgagaaaca atttttaaaa caatgacctg aaaataaaac aagatcgcag
    10621 caaaatgacg ttttaaatgc cgctttgctg cgatcttttc catatgatct aacttaattt
    10681 tccagataaa tttttcagat actctnnnnn nnnnnaattc atctgctaca aactgtacct
    10741 gtgttccgat gataacctgt acagaggtct tgcttggacg catcacacct gcaacaccgg
    10801 cagatttgat cactttttcg ttgacctttg tatagtcttt tacttccaga cgaagtcttg
    10861 taatacagtt gtcaatggaa gccacatttt caggaccgcc aacgccttcc aggactgtct
    10921 ttgcaacaga agtataatca ttgtttgcta aagttgcctt taactcttct gca
//
LOCUS       DBMDUA010000088        10912 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_2982_length_10912_cov_2.904079, whole genome shotgun sequence.
ACCESSION   DBMDUA010000088 DBMDUA010000000
VERSION     DBMDUA010000088.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10912)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10912
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2982_length_10912_cov_2.904079"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="ACZPCL_07960"
     CDS             <1..284
                     /locus_tag="ACZPCL_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162434.1"
                     /translation="RLQSFCIQRSIQLLIIESIKEESEMSSIKTDIASKFQKAMDTFQ
                     DVSEEDGDQDYLKLFSFICELSFDVYMKMQMIEQLTDQLRAERPDPKKK"
     gene            549..3137
                     /gene="xdh"
                     /locus_tag="ACZPCL_07965"
     CDS             549..3137
                     /gene="xdh"
                     /locus_tag="ACZPCL_07965"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HIR1162435.1"
                     /translation="MSTFFVNGKSVTTTRKQSLLRFLRDELKLTSVKDGCSQGACGAC
                     TVIIDGETCRACVPTTDTLEGRNIITVEGLTDWESKVYTYAYGEAGAVQCGFCIPGMV
                     MCTKALLDKNPDPTEAEIRYAIRNNYCRCTGYVKIIAAIRLAAKIKREGIIPEDSATD
                     WKIGSRVHRLDVEEKVLGYGKYPDDFYFDNMCYGSAVRSKYPRARVLSIDISAAKALP
                     GVVAVLTAEDVPGENLVGHLKHDQYTLIPVGGLTHYLGDAIALVVAEDRETLEKAKKL
                     VKVEYEVLPAIHNIEEAAAPDAPLVYDEETSNVQAYKHVCRGNAEEAIKHAAHVISHH
                     FETPWTEHAFLEPECVVSYMDSDGDVFLITTDQSSHTTLHECTRLLGSKKVKVQNALV
                     GGGFGGKEDMSVQHHAALMTYCTGRAVKVSLTRAESLLVHPKRHHFVMDFTMGCDENG
                     KIMGVKAKVASDTGAFASLGGPVLERACTHAAGPYAYENFEIEGTAYYTNNPPAGAFR
                     GFGVTQTCFAVETLLNMMADEIGITPWEIRYRNAIRPGGVLPNGQIVDNSTGLVETLE
                     AVKEEYDAAVASGKAVGLACAMKNAGVGVGIPDWGRVKLIVEDDAKLHIYTGASCIGQ
                     GLGTVLVQMVVTNTDLARDQIVYERSNTWIAPDSGDTSGSRQTLVTGEACRRACEKVM
                     EAKNASEHHSLDDLKGQVFYGEYLAKTDPLGADVPNPVSHVAYGYATQMCIIDPKTKK
                     IEKMVAAHDVGKAVNPLSCEGQIEGGVVMSMGYALRERYPIDDNCKPIEKYGSLGLFR
                     AHEVPEIEAIVIDKPGLNVACGAIGIGEITSIPTAPAITDAYYRCDGERRYSLPISGT
                     PYERRF"
     gene            3140..3628
                     /locus_tag="ACZPCL_07970"
     CDS             3140..3628
                     /locus_tag="ACZPCL_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1162436.1"
                     /translation="MAVKKKFPYRSAFVACNGGCHVTEGSSCSYGCIACGKCVESCKF
                     GAIHLNEHGVAEVDEEKCIACGRCTRECPQHIIHLHDCANTIAVKCSNKDKDKDARGV
                     CAVSCIGCGICEKTCTASAIHVEDNCAVIDEASCLSCGMCAVKCPRHAIYDLRGIFTK
                     VR"
     gene            complement(3852..5234)
                     /gene="hydA"
                     /locus_tag="ACZPCL_07975"
     CDS             complement(3852..5234)
                     /gene="hydA"
                     /locus_tag="ACZPCL_07975"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HIR1162437.1"
                     /translation="MRQIIKNGTIVTGSGSFPADILIDGEKIAATGTAEEIKKLAQPG
                     DKEIDASGCLIFPGFIDAHTHFDLHVAGTVTADDFATGTKAALKGGTTTIIDFGTQYP
                     GETLAEGLKNWHEKADGKCSCDYSFHMSITDWNPSVSKEVQDMMDEGITSFKLYMTYD
                     TQVDDKTLFEILQRLKETGGITGVHCENSGMIAALQEEAKAAGKMGVSSHPKTRPVAA
                     EAEAIGRLLRLAEVADVPVVVVHLTCKEGYDVIAQARKRGQKVYVETCPQYLLMDDSL
                     YELPGFESAKYVCAPPLRKKEDQDCLWKGLADGTIQTVSTDHCSFTTEQKKLGEGDFT
                     KIPGGMPGAETRGTLIYTYGVDKGRITKEDMCRVLSENPAKLYGLYPRKGVIAPGSDA
                     DLVIMRTGVEDVITAKDQVQNVDYAPFEGTKVTGRVESVFLRGLQAVKDGKVVEEKKG
                     EFLKRGKYAL"
     gene            complement(5250..5489)
                     /locus_tag="ACZPCL_07980"
     CDS             complement(5250..5489)
                     /locus_tag="ACZPCL_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HIR1162438.1"
                     /translation="MPREKTMKVVFAFPTTTMAMKMEAAAKAKEAPGRLIPVPRQISA
                     GCGMAWCAPVADRVSLEQLIATEQIVTEGCYELLL"
     gene            complement(5642..8629)
                     /gene="ygfK"
                     /locus_tag="ACZPCL_07985"
     CDS             complement(5642..8629)
                     /gene="ygfK"
                     /locus_tag="ACZPCL_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HIR1162439.1"
                     /translation="MSDIMTPIPFGKMMNWILEEHKKGAIFGIRRPFVADQSKKYEIF
                     GRTLETPFGPAAGPHTQLTQNIVAAYVAGSRFFELKTVQKLDGEDLPVAKPCIKADDE
                     CYNCEWSTELYVPQAYDEYVKAWFACKVLAKEYGLGSMDGFQFNMSVGYDLDGIKLEK
                     VDRFIEGMKDASAAPIFNECRQWLLDNLDRFDNLTKEDVESISPEICNCATLSTLHGC
                     PPQEIERIASYLLTEKKVHTFIKCNPTLLGYEYARKLMDDMGYDYVAFGDFHFRDDLQ
                     YTDAVPMLQRLQKLADEKGLEFGVKITNTFPVDVKQNELPSEEMYMSGKSLYALSMSV
                     AQKLAKDFDGKLRISYSGGADYFNITKIVDAGIWPVTMATTMLKPGGYERLEQIGQLF
                     KAKEAAAFAGVSAEKVEAMVEAAKSDKHHVKAVKPLPSRKVKKPVPLTDCFIAPCQEG
                     CPIHQDITRYMQLAGEGKYEEALKVILNKNPLPFITGTICAHNCMSKCTRNFYEASVD
                     IRRTKLESAQGGIDAVMAALKAPAVTSDKKAAVVGGGPAGLAAAYFLAKGGMKVTVFE
                     KAEKMGGVVRNVIPGFRISHEAIDHDVELVRAMGAELVNGKEITSVDELKKEYDYVVL
                     AVGASEPGRLRLEAGETMNALEFLAQFKATDGKVDLGKNVVVIGGGNTAMDTARAAKR
                     NAGVEKVSLVYRRTKRYMPADEEELVMAIDDGVEFAELLAPVKLENGELICRKMQLGD
                     YDASGRRSVVETEEIVKIAADTVIAAVGEKVPGAFYEANGIAVDEKKRPVVDHNTLET
                     SVKGVYVAGDGLFGPATVVEGIRDGKKAAEAIIGRDLSEDIFKMADAEVVYGRKGKLS
                     EENEESDSRRCLSCNSICENCVEVCPNRANVTLTVPGMDKHQVIHVDYMCNECGNCRS
                     FCPWDSAPYLDKFTLFANEADMENSKNQGFTVLDAAAGICKVRLAGNVIDYTVGTANE
                     NVPDGIQKIIKTVISDYSYLLIG"
     gene            complement(8971..10302)
                     /gene="ssnA"
                     /locus_tag="ACZPCL_07990"
     CDS             complement(8971..10302)
                     /gene="ssnA"
                     /locus_tag="ACZPCL_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HIR1162440.1"
                     /translation="MLIIGNGRLVTRDAGQPFFENGAVAIDGTTIKKVGTLEEIKKEF
                     PEAEFVDAKGGVIMPAFINTHEHIYSAMARGLSIKGYDPHGFLDILDGLWWTIDRHLT
                     NEQTRQSARVTFIDSIKNGVTTVFDHHASFGQIKDSLFAIEDAAKEFGVRACLCYEVS
                     DRDGMDKSREAVLENARWIKHALADDTDMFAGMMGMHAQFTISDETMEFAAANKPDEV
                     GYHIHVAEGIEDLHDCLHKYGKRIVDRLYDCNILGSKTLLGHCIYVNPHEMELIKATD
                     TMVVHNPESNMGNACGCPPTMEIVHRGILTGLGTDGYTQDVLESYKVANVLHKHHLCD
                     ANAAWGEVPQMLFEGNAKIANRYFKKQLGVLKEGAAADVIVTDYVPRTPMDASNINSH
                     ILFGMTGRSVVTTVCNGKVLMKDRELIGIDEEKVLYEVREEAKKLERSING"
     gene            10549..>10912
                     /locus_tag="ACZPCL_07995"
     CDS             10549..>10912
                     /locus_tag="ACZPCL_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR1162441.1"
                     /translation="MAYTLSLLEQIADGLARQFGPDCEIVIHDLKRHDLEHSVVHINN
                     GHITNRREGDGPSKVVLETLHRDPASLKDHLGYLTRTSNGKILKSSTFFIRNPEDSAV
                     DYIFSINYDITGLMTIERS"
BASE COUNT         2810 a         2692 c         2259 g         3151 t
ORIGIN      
        1 tccggctcca atccttctgc atccaaagaa gcatccaact tcttatcata gaaagtataa
       61 aggaagaaag tgaaatgtcc tctattaaaa cagatatcgc ctctaaattc cagaaagcaa
      121 tggatacatt ccaggatgtt tctgaggaag acggcgacca ggattacctg aaattatttt
      181 cctttatctg tgaactttcc tttgatgtgt atatgaaaat gcagatgatc gagcagctga
      241 cagaccagct tcgtgcagaa cgtccggacc ccaaaaagaa atagtttttt ctcccacaaa
      301 aataattttt ccatatttta ccaattacat ccccgatttc tgaaggtgac ttacgttatc
      361 ttcaaaaacc ggggattttc gttattctcc cttgaaataa gcgcttacaa tttttttgtt
      421 catacataaa tttttttgag attgttgttg ctttttgttt attttatgat aaaataaaag
      481 tataaatggt cggtttatta ttcgttttaa caacttattt gttcctacct tgaaaggaga
      541 ttatacatat gtctacattc tttgtaaacg gcaagtctgt gacaactaca cgtaagcagt
      601 ccctgttacg gttcctgcgt gacgagctga aactgacttc cgtaaaagac ggatgcagtc
      661 agggtgcctg cggtgcctgt accgttatca ttgacggtga aacctgtcgc gcctgtgttc
      721 ctaccacgga caccttagag ggccgcaaca ttattaccgt agaaggcctt actgactggg
      781 aatccaaagt ttacacctac gcttacgggg aagcaggtgc tgtacagtgc ggtttctgta
      841 tccctggcat ggttatgtgt accaaagcac tgttagataa aaatccagat ccgacagaag
      901 ctgagatacg ctatgccatc cgcaacaact actgccgctg taccggttat gtaaagatca
      961 ttgctgcgat ccgtctggca gccaagatca aacgtgaagg aattatcccg gaagacagcg
     1021 caactgactg gaagatcggt tccagagtcc atcgtctgga cgtagaagaa aaagttttag
     1081 gatatggcaa atatccggac gacttctatt ttgacaacat gtgctatggc tctgctgtac
     1141 gaagcaaata tccaagagct cgtgtgcttt ctattgatat ttctgcagca aaagcacttc
     1201 ctggtgttgt ggctgtactt actgcagaag atgttcctgg tgaaaatctg gttggacact
     1261 taaagcatga ccaatataca ctgatcccgg taggcggtct gacccattat ctgggagatg
     1321 ccattgccct ggtggttgct gaagacagag aaactctgga gaaagcaaag aaactggtaa
     1381 aagttgagta tgaggtactt ccggctatcc acaacattga agaagcagct gcaccagatg
     1441 cacctttagt atatgatgag gaaacttcca atgtacaggc ttacaaacat gtctgtcgcg
     1501 gcaatgctga ggaagccatc aaacatgcag cacacgttat ttcccatcac tttgagactc
     1561 cgtggactga gcatgctttc ctggaaccgg aatgtgttgt ttcctatatg gacagtgacg
     1621 gcgatgtgtt cctgatcact actgaccagt cttcccatac tacccttcac gaatgtacca
     1681 gactgttagg gagcaaaaaa gtcaaggtac agaatgcctt agttggcggc ggtttcggcg
     1741 gcaaagagga tatgtccgtt cagcatcacg ctgcactgat gacctactgc acaggccgtg
     1801 ctgtaaaagt aagccttaca agagcagagt ctctcctggt acatccaaag cgtcatcact
     1861 ttgttatgga tttcaccatg ggctgtgatg aaaacggtaa gatcatgggc gttaaggcaa
     1921 aagttgcttc tgatacaggt gctttcgctt cccttggcgg tcctgttctg gaacgtgcat
     1981 gtacccacgc tgcaggtcct tatgcttatg aaaactttga gatcgaggga actgcttatt
     2041 atacaaacaa cccgccagcc ggtgctttcc gcggtttcgg cgtaacacag acctgttttg
     2101 cagtagaaac tcttctgaac atgatggcag atgagatcgg tatcactcct tgggagatcc
     2161 gctaccgcaa tgccatccgt ccgggcggcg ttcttccaaa cggtcagatc gtagataatt
     2221 ccaccggtct ggttgaaaca ctggaagctg taaaagaaga atatgacgct gctgtagctt
     2281 ctggtaaagc cgttggtctt gcctgcgcta tgaagaatgc cggtgtcggc gttggtatcc
     2341 ctgactgggg ccgtgtaaaa ctaatcgtag aagatgacgc aaaactgcac atctataccg
     2401 gtgcttcctg tattgggcag ggacttggta ccgtcctggt acagatggta gttacaaata
     2461 ctgacctggc aagagaccag atcgtatatg agcggagcaa cacctggatc gcacctgact
     2521 ctggtgatac ttccggttcc cgccagactt tagttaccgg tgaagcctgc cgccgtgcct
     2581 gcgaaaaggt aatggaagca aagaatgcca gcgaacatca ttccttagat gacctgaagg
     2641 gacaggtatt ctacggtgaa taccttgcaa agacagaccc gttaggagct gacgtaccaa
     2701 accctgtatc ccacgtagct tacggttatg ctacccagat gtgcatcatc gatccaaaga
     2761 ccaagaagat cgagaaaatg gttgctgccc atgatgtagg taaggcagta aacccattat
     2821 cctgcgaagg ccagatcgag ggcggtgtcg ttatgtccat gggatatgct ttaagagagc
     2881 gttatccaat tgatgacaac tgcaagccta ttgaaaaata tggttcttta ggtctcttcc
     2941 gcgctcatga agtacctgag atcgaagcca ttgttattga caaacctggt ctgaatgtag
     3001 cctgcggagc catcggtatc ggagagatca cttctatccc tactgctccg gccattacag
     3061 acgcttacta tcgctgtgac ggagaacgcc gctacagcct tccaatttca ggaacacctt
     3121 atgaaaggag attctaatca tggctgttaa aaagaaattt ccatatcgtt ctgcttttgt
     3181 agcctgcaac ggcggatgtc atgtaacaga aggctccagc tgttcctatg gctgtatcgc
     3241 ctgcggaaaa tgtgtagaaa gctgtaaatt cggtgccatc catctgaatg aacatggcgt
     3301 agctgaagta gatgaagaaa aatgtatcgc ctgcggacgc tgcaccagag aatgtcctca
     3361 gcacatcatc cacttacacg actgtgccaa taccattgct gttaaatgtt ccaacaaaga
     3421 taaagacaaa gacgcaagag gtgtctgtgc cgtcagctgt atcggctgcg gcatctgcga
     3481 aaagacctgt actgccagtg ccatccacgt agaagacaac tgcgcagtga ttgatgaagc
     3541 atcctgctta agctgcggta tgtgtgcagt gaaatgtcca cggcatgcaa tctatgattt
     3601 gcgaggaatt tttacgaaag tgagataaat atagcattgg aaccccgcct ggcagcgggg
     3661 tttcgtgcat atactcagga tttttccgct gttggcggaa gacttgcaat ttcccagctg
     3721 ctgacgggct tgcgtgcata tacacacgct ttcccgccgc caggcggaga aagtcagctg
     3781 ccataacaaa aaaacgggcc cggcagccac attagtcagc tgccagagcc cgttttctca
     3841 ctcactctat tttacagagc atattttcct ctcttcagga actcgccctt cttctcttct
     3901 accacttttc cgtcctttac agcctgaagt ccacgtaaga acacgctttc cactcttccg
     3961 gtcacttttg taccttcaaa tggtgcataa tctacattct ggacctgatc tttggctgtg
     4021 atcacatctt ctacacctgt acgcatgatc acaaggtctg cgtcacttcc tggagcgatc
     4081 acgccctttc tcgggtataa gccatacagt tttgcaggat tttcagacag aacgcgacac
     4141 atatcttctt ttgtgatacg acccttatct acaccgtaag tatagatcaa agtccctctt
     4201 gtctcagcac ccggcatacc gcctgggatc ttggtaaagt cgccttctcc taacttcttc
     4261 tgctctgtgg taaagctgca gtggtcagta gaaacagtct ggatggtacc gtctgccaga
     4321 cctttccaca agcagtcctg gtcttctttc tttcttaaag gcggcgcgca cacatatttt
     4381 gcactttcaa agccaggaag ttcataaaga ctgtcatcca tcagcagata ctgtggacag
     4441 gtctccacat aaaccttctg tcctctcttg cgggcctgtg caatcacatc atatccttct
     4501 ttacaggtca gatgcactac aactacagga acatctgcta cttctgccag acgtaacagt
     4561 cttccaattg cttctgcttc cgcagctacc ggtctggtct ttggatggct ggaaacaccc
     4621 atttttcctg ctgcctttgc ttcttcctgt aaagctgcaa tcataccgct gttttcacag
     4681 tgtacaccgg tgataccgcc agtctctttt aatctctgta agatctcaaa taaggtctta
     4741 tcatctacct gggtatcata agtcatatac agtttaaaag aagtaattcc ttcatccatc
     4801 atatcctgga cttctttgga aacagaaggg ttccagtctg taatagacat atggaagctg
     4861 taatcgcagg aacactttcc atctgccttt tcatgccagt tctttaagcc ttctgccagt
     4921 gtttctcccg gatactgggt tccaaaatcg ataatggtag tagtaccgcc ttttaaagca
     4981 gcctttgtac cggtagcaaa atcatctgct gttacagttc ctgccacatg caggtcgaaa
     5041 tgggtatgtg catcaataaa tccagggaaa atcaggcatc cagatgcatc gatctcctta
     5101 tctcccggct gtgccagttt tttaatctct tcagctgtac ctgtagccgc gatcttttcc
     5161 ccatcaatga ggatatctgc aggaaaactt ccgcttcctg tgactatagt tccgttttta
     5221 atgatctgtc tcatattatc cagcctcctt tataataaca gctcatagca gccctctgtt
     5281 acgatctgtt ccgtagcgat cagctgctcc agagaaactc tgtccgcaac cggtgcgcac
     5341 caggccattc cacagcctgc gctgatctgc cttggaaccg ggatcagacg tcccggagcc
     5401 tcttttgctt ttgcagctgc ttccattttc attgccattg tggtagttgg aaatgcaaat
     5461 accaccttca ttgtcttttc tctgggcatg ttatcttcct ctctcgctca gatccacacg
     5521 tacacatgct gacatgcttc actgcctgct tacgcaggcg ggatcttcat taatggcttg
     5581 tgaaaaaacc tctagggcat gaccaaaaag ccacgccccg aagttttttt cttttataaa
     5641 attaaccgat cagcagatag ctgtaatcag aaattacagt cttaatgatc ttctgaattc
     5701 catccggaac attctcattt gcagttccaa ctgtgtaatc aattacattg cctgctaaac
     5761 gtaccttgca gattcctgct gctgcatcaa gaaccgtgaa gccctggttc ttgctgtttt
     5821 ccatatctgc ttcatttgca aataaggtaa acttgtcaag atatggagcg ctgtcccatg
     5881 ggcagaagct gcggcagttg ccacattcat tacacatata atctacatgg atcacctgat
     5941 gtttgtccat gccaggtact gttaaggtta cgtttgctct gttcgggcat acctctacgc
     6001 agttttcgca gatgctgttg caggagagac atcttctgct gtcagactct tcattctcct
     6061 cagacagttt gcccttacgt ccgtaaacaa cctcagcatc agccatcttg aagatatcct
     6121 cagataaatc tcttccgata atagcttctg ctgctttctt accatctctg atgccttcta
     6181 caacagtagc tggtccaaag agaccatcgc cggcaacata aacgcccttt acagaggtct
     6241 ccagagtgtt gtggtcaaca actggacgct tcttttcgtc tacagcgata ccatttgctt
     6301 cgtagaatgc gcctggaacc ttctcaccaa cagctgcgat cacagtgtca gcagcgatct
     6361 taacgatctc ctcagtctca acaacgcttc ttcttccgct tgcatcgtaa tcacctaact
     6421 gcatcttgcg gcagatcagt tcgccatttt ccagtttaac aggtgctaac agctctgcaa
     6481 actctacgcc gtcatcaatt gccattacta actcttcttc atcagctggc atatatctct
     6541 tggttcttct gtaaaccagg gatacctttt ctacgcctgc attgcgcttt gcagctcttg
     6601 cagtatccat agctgtgtta ccgccaccga taacaacaac gttcttacct aaatctacct
     6661 tgccgtcagt tgccttaaac tgcgccagga actctaatgc gttcatggtc tcgccagctt
     6721 ccagtctcag tctgcctggt tcagaagcac caactgccag tacaacgtag tcatattctt
     6781 tctttaattc atcaacagaa gtgatctctt tgccgtttac cagctctgca cccatagcac
     6841 gaaccagctc aacgtcatga tcgattgctt cgtgggaaat acggaagcct gggatcacat
     6901 tgcgtacaac gccgcccatc ttttctgcct tctcaaatac agtaaccttc ataccgccct
     6961 ttgcaaggaa gtatgcagct gccagaccag caggaccgcc gcctactaca gctgccttct
     7021 tatcagaagt aactgcaggt gcctttaatg ctgccataac agcatcaatt ccgccctgtg
     7081 cagactctaa cttggttctg cgaatatcta cagaagcctc gtagaagttt ctggtacatt
     7141 tgctcataca gttatgtgca cagatggtac cggtgataaa tggaagcggg ttcttgttta
     7201 agataacctt taatgcttct tcgtactttc cttcacctgc taactgcatg taacgggtga
     7261 tatcctgatg gatcgggcat ccttcctgac atggagcgat aaagcagtct gttaacggaa
     7321 ccggcttttt cacctttctg gatggaagcg gcttaacagc ctttacatgg tgtttgtcag
     7381 actttgcagc ctctaccata gcttctacct tctctgcgct tacgccagcg aaagctgctg
     7441 cttcttttgc cttaaataac tgtccgatct gctctaatct ttcatagccg cctggcttta
     7501 acatggtagt tgccatggtt acaggccaga taccagcgtc tacgatcttt gtgatattaa
     7561 agtaatcagc accgccggag taggaaatac gcagttttcc gtcaaagtct tttgccagtt
     7621 tctgtgcaac agacatggac agagcgtata aagacttacc ggacatgtac atttcttcac
     7681 ttggaagttc attctgtttt acatcaacag ggaaggtatt tgtgatctta acaccaaatt
     7741 ccagtccctt ctcatctgcc agcttctgca gtctctgtaa cattggaact gcatctgtgt
     7801 actgaaggtc atctctgaaa tggaagtcac cgaacgctac ataatcatag cccatgtcat
     7861 ccattaattt tcttgcatac tcatatccta acagagttgg attgcacttg ataaaggtat
     7921 gtactttctt ctcagtgagc agatagcttg cgatacgctc gatctcctgt ggaggacatc
     7981 cgtgcagagt ggagagagtt gcacagttgc agatttcagg ggagatactt tcaacatcct
     8041 cttttgtcag gttatcaaaa cgatccaggt tatctaacag ccactgacgg cactcattaa
     8101 aaataggtgc tgcagaagca tccttcattc cttcaataaa acggtctacc ttttccagtt
     8161 tgatgccgtc caggtcataa ccaacggaca tattaaactg gaagccgtcc atagagccaa
     8221 ggccatattc cttagctaat actttacagg caaaccatgc tttcacgtac tcatcatatg
     8281 cctgcggcac ataaagctca gtggaccatt cacagttgta gcactcatca tcagccttga
     8341 tacatggctt tgctaccgga aggtcttcac catctaactt ctgaactgtc tttaattcaa
     8401 agaagcggct tcctgccaca taagcagcta caatattctg ggtcagctgg gtgtgtggtc
     8461 ctgcagcagg tccgaaaggt gtctctaatg tacgtccaaa gatctcatat ttcttgctct
     8521 gatctgcaac aaaaggtctt ctgatgccga agatcgcgcc tttcttgtgt tcctccagga
     8581 tccagttcat catttttcca aagggaatgg gtgtcataat atcgctcata ttctatttcc
     8641 tccttatata tagcacccga tcaggtccca gcaccgtagg ggggcttctg caaaattttt
     8701 gtatctttgc agaagccagt ttttatcttt caacggctca gccgcccgga tattccggtg
     8761 tgctgttttc ccgccgccct gtaatcggct attactaatt aatttacatc agttcaacca
     8821 tgcgctgatt tgaatgagaa agcgtgttgc aagttcgctt gcataaacac tttcttgttc
     8881 aaatcagcat atggtggatc caccaagcct cttgttttca tgaatatgga aacaagaaga
     8941 tggcatggtt gaactgtaac atttacatta ttaaccgtta atggaacgtt ccagcttctt
     9001 agcctcttct cttacttcgt aaagaacctt ctcttcatca attccgatca gctctctgtc
     9061 tttcattaag actttaccgt tgcagactgt ggtaacaacg ctgcgtccgg tcataccgaa
     9121 taagatgtgg ctgttgatat tagaagcatc cataggtgtt ctcggtacat aatctgtaac
     9181 gatcacatct gctgctgcgc cttcttttaa tacgcctaac tgcttcttga agtagcggtt
     9241 tgcgatctta gcatttcctt caaagagcat ctgaggaact tcgccccatg ctgcatttgc
     9301 gtcacacaga tgatgcttgt gaagtacatt tgctacctta taagactcca gaacatcctg
     9361 tgtataacca tctgttccaa gacctgtcaa gatacctctg tgaacgatct ccatggttgg
     9421 agggcatccg caggcattac ccatgttaga ctcagggttg tgtactacca tagtatcagt
     9481 tgccttgatc agttccatct catgtgggtt tacatagatg cagtggccaa gtaaagtctt
     9541 gcttcctaag atattgcagt cgtataaacg gtctacaatg cgctttccat atttatgtaa
     9601 gcagtcatga agatcttcga taccttctgc aacatggata tgatatccaa cttcatctgg
     9661 tttgtttgct gcagcaaatt ccattgtctc atctgaaatg gtaaactgtg catgcatacc
     9721 catcatacca gcaaacatat ctgtatcatc tgctaatgca tgcttgatcc atcttgcatt
     9781 ctctaataca gcttctctgg acttgtccat accgtcacgg tcagatactt cataacacag
     9841 acaggctctt acaccaaatt cttttgcagc atcttcgatg gcaaacaggc tgtccttgat
     9901 ctggccaaag cttgcatgat ggtcaaatac agtggtaaca ccgttcttga tactgtcgat
     9961 gaaagtaact cttgcgctct gacgggtctg ctcattggtc agatggcggt cgatggtcca
    10021 ccataatccg tctaagatat ctaaaaatcc gtgaggatca tatcctttaa tgcttaagcc
    10081 tcttgccatt gcactgtaga tatgctcatg tgtattaatg aaagccggca tgataacgcc
    10141 ccccttagcg tctacaaact cagcttccgg aaactccttt ttgatctctt ccagagttcc
    10201 tactttctta atagtagttc cgtcaattgc tactgctcca ttttcgaaaa atggctgtcc
    10261 ggcatctctt gtaaccagac gtccgtttcc aatgatcaac atatggctct ctccttttcc
    10321 tatataataa caatgttgtc gtactcttat attaacacct ggaaatacca tttgcaaggg
    10381 aaaaatacaa ttttttttag ttattcaaaa tttttttgta aaaactattg caatctgttt
    10441 tatttgtgct attatgtaat cataaacaac gcaaaaatgt tttgcagatt gtttattgta
    10501 caaagttacc atatcatatt atgaaagtcc gaaatgagga ggttgttcat ggcatacaca
    10561 cttagcttgc ttgaacagat tgcagacggc cttgcaagac agttcggacc agactgcgaa
    10621 attgtgatcc acgacttaaa gcgccacgac ctggaacact ctgttgtaca catcaacaac
    10681 ggacatatca ccaaccggcg tgaaggcgac ggaccatcta aggtagttct ggaaaccctg
    10741 catagggatc cggcttcact gaaagaccat cttggttatc ttacccgcac atcaaacggt
    10801 aagatcttaa aatccagtac gttttttatc agaaacccag aggattccgc tgtggattac
    10861 attttttcca tcaactacga tatcaccggt ctgatgacca ttgagagatc ca
//
LOCUS       DBMDUA010000089        10796 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3016_length_10796_cov_3.484712, whole genome shotgun sequence.
ACCESSION   DBMDUA010000089 DBMDUA010000000
VERSION     DBMDUA010000089.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10796)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10796)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10796
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3016_length_10796_cov_3.484712"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(129..938)
                     /locus_tag="ACZPCL_08000"
     CDS             complement(129..938)
                     /locus_tag="ACZPCL_08000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1162442.1"
                     /translation="MDYKIIVLDLDGTLTNRDKVITPRTKEALMKAQEAGKIVVLASG
                     RPTAGVEPLAKELDLARFGSYILSYNGGMITNCKTGEVIFSSLLPVESNKKIMDLAKE
                     YGVDFLTYEGEEIITNNDQCVYAHKESKINHLPLRQVDNMEEHVNFPVAKFLLLDDGD
                     YLATVEPKVKAAMGKNFAVYRSEPYFLEVLPKGIDKAQSLERLLQHLGMTKEEMIACG
                     DGYNDLTMIQYAGLGVAMENAVLPVRKAADYITASNNDDGVGLVVEKFMLA"
     gene            complement(1187..2242)
                     /locus_tag="ACZPCL_08005"
     CDS             complement(1187..2242)
                     /locus_tag="ACZPCL_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1162443.1"
                     /translation="MKKWNVYAAAVLAAVGILGGCGAKNAESQAPAETQAAEQSAQES
                     SVEESRETSAEESKTETEKNGTLSVTYVKSPLNVPSIVELDQEIFASVFGPMGYDVQY
                     SDLTTGPEQTQALASGDIQFLNAVGATSVILSASNDADIKIMSIYSRSPKAFKLFAKD
                     DSIKTAEDLKGKKVAGPKGTILHELLVSYLNNAGMTEDDIEFMAMGIPDAQAALAGGS
                     VDAALLAGPTAYNMEKDGFYIVTDGEGLTEATIVTATSEKFYEEHPELVKAFLTAQKQ
                     VLDEMDADHAAAVSATAKATGLEEAAVEEMYGLYDFDMEIKDSDIEAMEKTEKFMEDT
                     GMIENPVDVASLILDVE"
     gene            complement(2275..3033)
                     /locus_tag="ACZPCL_08010"
     CDS             complement(2275..3033)
                     /locus_tag="ACZPCL_08010"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1162444.1"
                     /translation="MWKIAEIRLKQLEKSFEVSKNSHPVLRRFSTDLDTEKITVILGR
                     SGCGKTTLLRVLAGLETLDGGQILFEQPEKLGVVFQEPRLMPWLTLEQNILFGADKKK
                     HSKSKLKEKAETLLRLTGLEKFGKAYPDQLSGGMMQRAALARALACDASYLLMDEPFA
                     ALDHFTRQTMQKEFLRICAEEKMGALFVTHSVDEALLLGDEILIMENGKVGSWFTIGE
                     NLAYPRDLLSEEMIGIKRKIIKTLENRSQTQKNF"
     gene            complement(3017..3802)
                     /locus_tag="ACZPCL_08015"
     CDS             complement(3017..3802)
                     /locus_tag="ACZPCL_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1162445.1"
                     /translation="MHKIRNKGTGKLKNFIKACVPALILLILWQLAFLSGKWSAYVLP
                     GPEKVWKSFVTMTGNGTLLKHVLVSFKRVLTGFSISFVCAFCLGMIAALKKDLEVYYG
                     PYVEFMRNVPPISLIALLILWFGIGETSKIIIIVLASFFPMFMNIRKGLLSCDKKLLE
                     VGKTCGLSKTACFFKIRLPWAFPDILVGMRIGLGYSWRAIIGAEMIAAGKGLGYLILD
                     AQQMSRSDKVIVGIFVIGLVGLLCDKAFLWIIRKWVRGGVENS"
     gene            complement(4229..5233)
                     /gene="yiaK"
                     /locus_tag="ACZPCL_08020"
     CDS             complement(4229..5233)
                     /gene="yiaK"
                     /locus_tag="ACZPCL_08020"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HIR1162446.1"
                     /translation="MKRVPYEVMVNEFERVLTKYGFEPKDAKASAELFAQNSMAGVYS
                     HGLNRFPRVVEYLKKGEIDPKVKAEKIMGFGAFERWDGHRGFGPLNAKLAMDRAVELA
                     KEFGMGIVALGNNNHWMRGGSYGWQAADQGCIGICWSNTCPNMPAWGGMDRKIGNNPL
                     IFAVPRSNGEHVVIDCAVSQFSYGKLEDCRLKGQQLPVPGGYDTNGNLTTDPGEIEKT
                     WRVLPMGYWKGSGISIALDLIATVLSNGNSVQKIGTFGDEVGLTQIMIAIDPTKGNSV
                     ELTDEIVNNIVADVKSSIPVKEGGEVYYPGELELLNIKQNKEEGIPVIEEKWEEVLNM
                     "
     gene            complement(5394..6407)
                     /locus_tag="ACZPCL_08025"
     CDS             complement(5394..6407)
                     /locus_tag="ACZPCL_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1162447.1"
                     /translation="MTIYDIAKQAGVSASTVSRVINNKPGINAQTRKRVQKLLNENHY
                     TPNEAARGLVMQSSKIIGILIEDLRIEHHTESAYVIEQEMTALGYTCITLSTGRRDEK
                     KADYIRILEQRRVDGAILMGSMFETESVKKSIKEHLPDVPVAIVNGYLDLPNVYGILI
                     DEERGVKDCAELMFKKGKKHLVMAVDSDTPSNRNKQKGYLRAMLEQGIAKEDIPFYTA
                     VNKEFTNPRDVRAAGAKLTEQILTERPETDGIIYTTDLLAVGGLEAAKKMGKRIPQDL
                     AIMGIDNTMYGEIVTPKLSSLDNKLVEVSQNASRAILDALDKKEVSHKVMLLTDIKER
                     ETT"
     gene            complement(6526..7545)
                     /locus_tag="ACZPCL_08030"
     CDS             complement(6526..7545)
                     /locus_tag="ACZPCL_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1162448.1"
                     /translation="MKKRYKALLAAMLTGALAAGCLAGCGKMDGTAGGKMKKVTLNEV
                     AHSIFYAPQYAAIELGYFEEEGIDLTLVNGAGADKVMTALISGDAQIGFMGSEASIYV
                     SQEGADDPAVNFAQLTQRAGNFLVGRTAQPDFKWEDLKGKKVLGGRAGGMPQMVFEYI
                     LKKHGIDPKTDLTIDQSISFGLTAAAFTSGDADYTVEFEPFATTLEMENCGTVVASLG
                     RDSGYVPYTAYCAKKSYMEKNPQLIQKFTNAIQKGLDYVNSHSAWEIAKTIQPQFKDT
                     PVEKIAAIIDRYKSQDTWKEDTIFEKDSFELLENILEESGELKKRVPYEDLVRTDFSI
                     NAAKK"
     gene            complement(7816..8946)
                     /locus_tag="ACZPCL_08035"
     CDS             complement(7816..8946)
                     /locus_tag="ACZPCL_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP-loop family protein"
                     /protein_id="HIR1162449.1"
                     /translation="MEINVGKLNELPEGSYQLIDMRSDRDFEYGTIPGAIHISQEDLQ
                     GNPQIIKSKKVILFCAHGTNSIEAAEELEAEGYRAYSLEGGYIAWLREEMRRQEAEDI
                     KNQVEQSIRKKFRKTIWSPFTKAVKQYELVKEGDKVAVCISGGKDSMLMAKLFQELKL
                     HNKFPFEVEFLVMDPGYSPDNRHVIEENARKLGIPVHIFESDIFDAVYTIEKSPCYLC
                     ARMRRGYLYSYAKELGCNKIALGHHYDDVIETILMGMLYGAQVQTMMPKLHSTNFESM
                     ELIRPLYLVREDDIKAWRDYNHLHFIQCACKFTDTCTTCNNEENRSKRVEIKELIREM
                     KKKNPFVEGNIFKSVENVNLDTVVCYKKDGERHWFLDDYDEK"
     gene            complement(9154..9759)
                     /locus_tag="ACZPCL_08040"
     CDS             complement(9154..9759)
                     /locus_tag="ACZPCL_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HIR1162450.1"
                     /translation="MCFDDLDGRNEFGRRPGCCGPDGGRGGNCGPCSGSPGNGGPDGC
                     GPGGWRPDPWRPGCGCTGRPGPMGPPGPRGPQGFPGYPGPQGETGATGETGPQGPAGE
                     TGATGPQGPQGPIGETGATGPQGPQGEMGATGPQGATGPQGPQGPEGPRGETGPQGPA
                     GPAGTVTPAAAVDDATSTEDIVTQFNLLLNSLREAGLLETE"
     gene            complement(9912..>10796)
                     /locus_tag="ACZPCL_08045"
     CDS             complement(9912..>10796)
                     /locus_tag="ACZPCL_08045"
                     /inference="COORDINATES: protein motif:HMM:NF040772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HIR1162451.1"
                     /translation="KCPYTYFSDIIIGETTCDGKKKMYELLNEIKETYVLHLPQSQER
                     PYAKDIWYEEVKLLKEKLEEKFNCTITEEGLRKAAHVRNELRKAQLDMYHLQKLVPPA
                     MKGTEMMIALQQGTFTFDVYQQIDNIRKRVTEAKKAYEEGKRPVPASAKRILLTGCPT
                     GGVIQKVGSVIENNGGVIVSLDDCSGERTNRLLVDENAPDILRAISDRYLSINCSVMT
                     SNNGRLENTAQMIRDYQVDGVVEIVLQACHTFNVEAFKMGRMAEEMGIPYMKLETDYS
                     TADSGQIETRIAAFIEML"
BASE COUNT         2532 a         2795 c         2133 g         3336 t
ORIGIN      
        1 tccgtaagaa gagccagggt atcctctaaa ttccgctctc tcagataatg cctgaaaaat
       61 tgttccagaa gttctctcat atgcgttttc atctcctgta tgattgcaga gttcctgcaa
      121 aaacgatctt aagccagcat aaatttctct actaccagtc ccacaccatc atcattatta
      181 gaagctgtaa tataatctgc tgcctttctt accggaagga cggcattttc catggcaact
      241 ccaagacctg catactggat catggtcaga tcattataac catccccaca ggcgatcatc
      301 tcttcttttg tcattcccag atgctgcagc aggcgctcta aagactgggc tttatcaatg
      361 cctttcggaa gcacttccag gaaataaggt tctgaacggt atactgcaaa attctttccc
      421 atggcagcct ttacctttgg ctctacggtt gccaggtaat ccccgtcatc taagagaaga
      481 aattttgcta ccgggaagtt tacatgctct tccatgttgt ctacctggcg cagaggaaga
      541 tggttgatct ttgactcttt gtgggcgtaa acgcactggt cattattggt aatgatctcc
      601 tcgccctcat aagtcagaaa atccaccccg tattcctttg ccagatccat gatcttcttg
      661 tttgactcca ccggaagcag ggatgaaaag atcacttctc ctgttttaca gttggtgatc
      721 atgccgccgt tgtaggataa aatatagctt ccaaaacgtg ccagatccag ttcctttgcc
      781 agtggttcca cgcctgctgt cgggcggccg gatgccagca ccacgatctt tcctgcttcc
      841 tgtgccttca tcagggcttc tttggtcctt ggtgtaataa ccttgtctct atttgtcaat
      901 gttccgtcca gatccaggac gatgatctta taatccatat ttatcctcac tttatcggtt
      961 tttcattttt aattaaatta aatgcagcaa aaaggctgtc ataaaatggt actaccactt
     1021 tataacagcc ccttcactca cgtccccccg cgctcatgca ctctactgcc tgctgacgca
     1081 ggcgggacct tcgttaatgg gccaggaaaa acaaatgcca ccttcggtgt cccttgtttt
     1141 tcttttgaac ccattatatc acatacagac tctataccac aatcatttac tctacatcta
     1201 agatcagaga tgccacatcg accggatttt cgatcatgcc tgtatcttcc atgaacttct
     1261 ctgttttttc catggcttca atatcactgt ctttgatctc catatcaaaa tcataaagtc
     1321 cgtacatttc ttctactgct gcttcctcta aaccggttgc ctttgcagtg gcggaaactg
     1381 ctgctgcatg atctgcgtcc atttcatcta acacctgttt ctgtgcagta agaaatgctt
     1441 ttaccagttc cggatgttct tcgtagaatt tttcactggt tgcagtaacg atggttgcct
     1501 ctgtaagacc ttctccgtct gtcactatat aaaatccgtc tttttccata ttgtaggcag
     1561 tcggacctgc aagaagagcc gcgtctacac ttccacctgc caaagctgcc tgggcatccg
     1621 ggatccccat tgccataaat tcaatatcgt cctcagtcat acctgcatta tttaagtaag
     1681 atactaacag ttcatgtaaa atggttccct ttggacctgc tactttcttt ccctttaaat
     1741 cttctgctgt cttaatgctg tcatcctttg caaacagctt aaatgccttt ggggaacggc
     1801 tgtaaatgct catgatctta atgtctgcgt cattggaagc agataaaatt acagaagtag
     1861 cgcctaccgc atttaaaaac tggatatctc cggaagctaa ggcctgtgtc tgctccggtc
     1921 ctgttgtcag atctgaatac tgcacgtcat aacccatcgg accaaataca ctggcaaaga
     1981 tctcctgatc cagctctacg atactgggaa catttaatgg agactttaca taggtaacag
     2041 acagggttcc atttttctct gtctctgttt ttgactcctc tgcactggtc tccctgcttt
     2101 cttccacgct gctttcctgt gcgctctgct ctgcagcctg cgtttctgcc ggtgcttggc
     2161 tttctgcatt tttagcaccg catcctccta aaattcccac tgctgccagc actgctgctg
     2221 cataaacatt ccattttttc atatttcatt tcctcccgtt ttttatgtta ggttttaaaa
     2281 gtttttttgc gtttgtgacc tgttttccag tgttttaatg atctttcgtt taatgccgat
     2341 catttcttct gacaacaggt ctctgggata tgccagattt tctccaatcg taaaccagct
     2401 tcctaccttc ccattttcca taataaggat ctcatcacct aaaagcaggg cctcatctac
     2461 gctgtgggtg acaaacaacg cccccatttt ctcttctgcg cagatcctta aaaactcctt
     2521 ttgcatggtc tgcctggtaa aatgatccag agctgcaaaa ggttcatcca tcagcagata
     2581 cgaggcatca caggctaatg cccttgccag agctgcccgc tgcatcattc ctcctgataa
     2641 ctggtccgga taagcttttc caaatttttc cagtcctgta agtcttaaaa gagtttctgc
     2701 cttttctttt aactttgatt ttgaatgttt ctttttgtct gccccaaata aaatattttg
     2761 ttctaaagta agccatggca tcaggcgggg ttcctgaaaa accactccca gtttttcagg
     2821 ctgttcaaaa aggatctgac cgccatctaa tgtttcaaga cctgccagga cccgcagaag
     2881 cgtggttttt ccgcagccac tgcgccctaa tatcaccgtg atcttttctg tatccagatc
     2941 tgtggaaaac cgtcttaaca ccggatgact gttttttgac acttcaaagc ttttttccag
     3001 ctgttttaat cggatctcag ctattttcca cacctccccg gacccatttt ctgatgatcc
     3061 acaaaaatgc cttatcgcaa agcagaccta ccaggccgat cacaaaaatc cctacgatca
     3121 ccttgtcgga gcgggacatc tgctgggcat ccaggatcag atatccaagg ccttttcccg
     3181 cagcgatcat ctccgcaccg ataatagccc gccagctgta tccaagaccg atgcgcattc
     3241 ccactaaaat atcaggaaat gcccagggaa gacggatctt aaagaaacag gcagtttttg
     3301 acaaaccaca ggtctttccc acttccaaaa gcttcttatc acaggaaaga aggccttttc
     3361 ggatgttcat aaacatggga aaaaaagacg ccagtacaat aatgatgatc ttggatgtct
     3421 ctccaatccc aaaccacagg atcaaaagag cgatcaaact gatgggcggc acattgcgca
     3481 taaattccac ataagggcca tagtatactt ccagatcttt ctttaaagca gcgatcattc
     3541 ccaggcagaa cgcacagaca aaagaaatgg aaaaacctgt caggaccctc ttaaaactta
     3601 ccagaacatg cttaagcaat gtcccgtttc ccgtcattgt aacaaaggac ttccacactt
     3661 tttccggtcc cggaagcaca taagcactcc attttcctga caaaaaggcc agctgccaga
     3721 ggatcagaag gatcagtgcc ggaacacagg cttttataaa attctttaac tttccagtgc
     3781 ccttattccg gatcttatgc aaaaaaatac cccctttctg ctttttgcga ctgtatgaca
     3841 cgttaaaagg cgcgaaatac agacacaaaa gcccaaagag ggtatttgtg tattctttta
     3901 tatgacagga aaagcagacc ccgttttagg ccgctgccag gaaatgacca gctgttctgg
     3961 gttgcaaaac aggcggaaaa ctccttgtct tctgtcattt tattttcagc gtctttcact
     4021 cagaaagtgc tctgcacagt ctttgtgtca gtcactttcc atcatacgct gaaatgagag
     4081 ctgcgtcaac tcttttaggg ggatttctta gactcaggtt tcccgcgctg acgcgctcta
     4141 ccgtctgctt acgcagacaa ttcgttcgtt aatggtccaa gaaaaacaaa tgccacctgc
     4201 ggtgtcgctt gtttttcttc tgggaccatt acatatttaa tacttcttcc cacttctctt
     4261 cgattaccgg gatgccttct tctttgttct gcttgatgtt taacagttcc agttcgcctg
     4321 ggtagtaaac ttctccgcct tcttttacag ggatggaact ctttacatct gctacgatgt
     4381 tgtttacgat ctcatcggtc agttctacgc tgttaccctt ggttgggtcg atagcgatca
     4441 tgatctgggt cagacctact tcatcgccga aggtaccgat cttctgtacg gagtttccgt
     4501 tggaaagtac agttgcgatc aggtcaagag cgattgagat accgcttcct ttccagtagc
     4561 ccattggaag tacacgccag gtcttctcga tctcgcctgg atcagtggtc aggttgccgt
     4621 tggtatcgta accgcctggt actggcagct gctgtccctt taagcggcag tcttccagtt
     4681 ttccatagga gaactgagat acagcacagt cgatcactac atgctcgccg ttgcttcttg
     4741 gtactgcaaa gatcagtggg ttgttaccga tcttacggtc cataccgccc catgcaggca
     4801 tattcgggca ggtgttggac cagcagatac cgatgcagcc ctggtctgct gcctgccagc
     4861 cgtagctgcc gccgcgcatc cagtggttgt tatttcccag tgcaacaata cccatgccaa
     4921 attcctttgc cagctctact gcacggtcca tagccagttt tgcatttaaa ggtccgaaac
     4981 cacgatgtcc atcccaacgc tcaaaagctc caaatcccat gatcttctca gcttttacct
     5041 ttggatcgat ctcgcccttc tttaagtact caaccactct tgggaaacgg tttaatccgt
     5101 gagaatatac accagccatg gagttctgtg caaacagctc tgcggaagcc tttgcatcct
     5161 ttggctcaaa accatacttt gttaatactc tctcaaattc gtttaccatt acctcataag
     5221 gaactctctt catgatctct ttctccttta tcttttatac tttctttgaa ttgctttttt
     5281 tcgcaatcga tttcagtgtt gttaaaagaa aatggttctt tttggaagaa ccttttcttt
     5341 tttataccag gcatctgtaa aaagctgtca atgacttttg ctttttgcaa atgctatgtg
     5401 gtttctctct ctttaatatc cgtcagcagc atcactttat gggatacttc ttttttatca
     5461 agagcatcta agatcgccct tgaagcattc tggctgactt ctaccagttt gttgtccaga
     5521 ctgctaagct ttggtgtaac gatctcgcca tacatggtgt tgtctattcc catgatcgca
     5581 agatcctgcg gaatacgctt tcccattttc tttgctgctt ccaggccacc tactgccaac
     5641 aggtctgtgg tatagataat accgtctgtt tcaggtcttt ctgtaaggat ctgttctgta
     5701 agttttgcac ctgccgccct cacatcacgg ggattggtaa attctttatt taccgctgta
     5761 tagaacggga tatcttcttt ggcgatcccc tgttctaaca tagctctcag gtagcctttt
     5821 tgcttgttcc ggttagatgg tgtatcggag tcaactgcca ttaccagatg tttctttcct
     5881 ttcttaaaca tcagttccgc acagtctttc acgccccgct cctcgtcaat gaggatgccg
     5941 taaacatttg gcagatccag ataaccgttc acaatggcta ccggaacatc cggaaggtgc
     6001 tctttaatgc tcttcttcac agattccgtc tcaaacatgg aacccataag aatagcaccg
     6061 tctacccgtc tctgctccag gatacggatg taatccgctt ttttctcatc ccttcgtccg
     6121 gtactcagag taatacaggt ataacctaag gcagtcatct cctgctcgat cacataagca
     6181 gactctgtat gatgctcgat ccgcagatcc tcgatcagga taccgatgat cttggaagac
     6241 tgcatcacca ggcctctggc tgcttcattg ggagtataat gattttcatt taataatttc
     6301 tgtacccttt tgcgcgtctg ggcattgatc ccaggcttgt tgttaatgac cctggaaacg
     6361 gtacttgcag acacacctgc ctgctttgca atgtcgtata tcgtcatatt ccctctcctg
     6421 tttctctgat gacttcatca tacgccttct tatagggttt tgtcaagagg tttttgcaat
     6481 cgatttcaac atttgttgca aataattgca aagcattctg tttgcttact tttttgctgc
     6541 attgattgaa aaatcagtac gcaccagatc ttcatatggc acccgttttt tcagttctcc
     6601 ggactcctct aaaatatttt ctaaaagttc aaagctgtct ttttcaaaaa tcgtatcctc
     6661 tttccaggta tcctgtgatt tgtagcggtc tataatagca gcaatttttt ccaccggtgt
     6721 atctttaaat tgaggctgga tggtctttgc gatctcccat gcagaatggg aatttacata
     6781 atcaagtcct ttctggatgg cattggtgaa cttctggatc agctgtggat ttttttccat
     6841 atagcttttc ttcgcacagt aagcggtata aggcacatag ccggagtcgc ggcctaaaga
     6901 tgctaccact gtgccacagt tttccatttc cagtgtagtt gcaaaaggtt caaattctac
     6961 cgtataatct gcatccccgg aagtaaatgc cgcagcagtc aatccgaaac tgatgctctg
     7021 gtcaatggta aggtctgttt tagggtcaat gccatgcttc tttaagatat attcaaatac
     7081 catctggggc ataccgcctg ctcttccgcc caaaactttc tttcccttca gatcttccca
     7141 tttaaaatcc ggctgggccg ttcttcccac caggaaatta cctgcccgct gggtcagctg
     7201 tgcaaagttc actgccggat cgtccgcccc ttcctgactt acataaatac ttgcttccga
     7261 ccccataaac ccgatctggg catcaccgga aataagggct gtcattacct tatctgcgcc
     7321 agctccattt accagagtca gatcgatccc ctcttcctca aaatagccaa gttcaatggc
     7381 agcgtattgg ggggcataaa aaatggaatg tgcaacttcg ttaagggtca cttttttcat
     7441 ttttccgcct gcagtaccat ccattttccc acatcctgca agacagcctg ccgcaagtgc
     7501 tcctgttaac atggctgcta aaagcgcttt atatctcttt ttcataaata tcctcctgct
     7561 tccgaaaata catcgaacac tatatgatat acggaaaagg agaaaatagt gaataaaata
     7621 aataagaatg tgccttggca cattctcgcg ccgagcgcgg tcgcttgcga caaactttat
     7681 catcgcgcga gtgcgcatcg ttggcaggtc gtgccttttt gtgtactagg gagttccaac
     7741 ggaatttcct agtacataaa aaaagactct atccttttgg aacagagcct ctctttacta
     7801 acttagtatt ttaatttatt tctcatcata atcatctaag aaccagtggc gttctccatc
     7861 tttcttgtag cagaccacag tatccagatt tacattttcc acactcttga aaatatttcc
     7921 ttctacgaat gggttctttt tcttcatttc gcggatcagt tccttgatct ccacgcgctt
     7981 ggaacggttt tcttcgttgt tgcaggtggt gcaggtgtcg gtaaacttgc aggcacactg
     8041 aatgaaatga agatgattgt aatctctcca tgccttgata tcatcttccc ttaccagata
     8101 cagaggacgg atcagttcca tgctttcaaa gttggtgctg tgaagctttg gcatcatggt
     8161 ctggacctgg gctccgtaga gcatgcccat gaggatggtt tcgataacat catcataatg
     8221 atgtcctaaa gcgatcttgt tgcatcctaa ttcctttgca taactgtaaa ggtatccgcg
     8281 gcgcattctg gcgcaaaggt aacaagggga tttttctatg gtgtagactg catcaaagat
     8341 gtcagactca aaaatatgga ccggaatacc taattttctg gcattttctt cgatcacatg
     8401 gcggttgtcc ggactgtatc ccgggtccat gaccagaaat tccacttcaa acgggaactt
     8461 gttgtgaagc tttaattcct ggaacagctt tgccataagc atagagtctt ttccgccgga
     8521 aatacagaca gctactttat ctccttcttt taccagttca tactgtttta ctgctttggt
     8581 aaacggggac cagatggttt tgcgaaattt tttgcggatg ctctgctcta cctgattttt
     8641 aatatcttcg gcttcctgtc tgcgcatctc ttcccgcaac catgcaatat agccgccttc
     8701 caggctgtag gcacggtagc cttctgcttc cagttcctct gctgcttcta tgctgttggt
     8761 tccatgggca cagaacagga tcactttttt tgattttatg atctgtggat taccctgcag
     8821 atcttcctgt gaaatgtgga tggctcccgg aatggttccg tactcaaaat cccggtcact
     8881 tctcatatcg atcaactgat aacttccctc cggaagttca ttcagttttc ctacgtttat
     8941 ttccatgttt atataacacc tttctgcttt tgaaatggtc acacaggctg ttacatggcc
     9001 tgcttttgta ctggaagtaa atataacacg aaatttcttt tgcttcaagt gtgttacatt
     9061 tttatataaa aagacgggtg gcttctgttt gtgagctgca gggtaagcaa aggccgccgt
     9121 accacaaatg gccggcggtc tttgaaatta cgtttattcg gtttcaagca gtcctgcttc
     9181 ccgcaggctg ttaagaagga gattgaactg ggttacaatg tcttctgtgc tggtggcatc
     9241 gtcaacagct gctgccggtg tgacggttcc cgccggacca gccgggccct gaggaccggt
     9301 ctcgcccctt ggaccttctg ggccctgggg gccttgtggg ccggttgctc cctgtggacc
     9361 tgttgctccc atctcaccct gtggtccctg aggcccagtt gctcccgttt cacctatcgg
     9421 accctgtggc ccctgcggtc ctgtagctcc tgtctctcct gccggccctt ggggaccggt
     9481 ctccccggtg gctcctgtct caccctgggg ccccgggtat ccgggaaatc cctgtggtcc
     9541 ccgtggtcct ggaggtccca ttggaccagg tctgcctgtg caaccacagc ctggtctcca
     9601 tggatccggt ctccatccgc ctggcccaca accatccgga ccgccatttc cggggcttcc
     9661 gctgcacggt ccacagtttc cgcctctgcc cccatccggt ccacaacagc cgggacgtct
     9721 tccaaattca ttccttccgt caagatcatc aaaacacata ttcaggctcc tttccaattc
     9781 cattttcatc catcttccgt ctctgtaaaa acagacgctt tattttcttt tgttactata
     9841 gaatatgtac aaggagtctt ttttgtgtcc atcttagcaa agcagccttt tccagtgatc
     9901 tacgatccgg tctataacat ctctataaat gctgcaatac gtgtttcgat ctgtccgctg
     9961 tctgctgtag aatagtcagt ttccagtttc atgtaaggaa tacccatttc ttcagccata
    10021 cggcccattt taaaggcctc cacattaaag gtatggcagg cctgaagaac gatctccact
    10081 actccgtcta cctgataatc cctgatcatc tgggcagtat tttccaaacg tccgttgtta
    10141 gaagtcataa cagaacagtt gatggacaga tagcggtctg aaatcgctct gagaatatcc
    10201 ggcgcgtttt catctaccag cagacggttg gtacgttcac cggaacaatc atccagactg
    10261 acgatcacgc caccattatt ttcgatcaca cttcccacct tctggatcac gccaccggtc
    10321 ggacatccgg taagcagtat cctttttgca gaagctggta ccgggcgttt tccttcttcg
    10381 taagcctttt tcgcctctgt tacccgttta cggatattat cgatctgctg ataaacatca
    10441 aaagtaaagg ttccctgctg aagggcgatc atcatttccg ttcccttcat ggctggcggt
    10501 accagtttct gcagatgata catatctaac tgtgcttttc gcagttcgtt gcgcacatgg
    10561 gctgcttttc gcagaccttc ttctgtaatg gtacagttaa atttctcttc cagtttctct
    10621 tttaacagtt ttacttcttc ataccagata tctttcgcat aaggacgctc ctggctctgt
    10681 ggaagatgaa gtacatacgt ttctttgatc tcatttaaca gctcgtacat cttcttttta
    10741 ccatcacagg ttgtctctcc gatgataata tccgaaaaat aggtataagg acattt
//
LOCUS       DBMDUA010000090        10754 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3028_length_10754_cov_2.498451, whole genome shotgun sequence.
ACCESSION   DBMDUA010000090 DBMDUA010000000
VERSION     DBMDUA010000090.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10754)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10754)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10754
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3028_length_10754_cov_2.498451"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(105..1736)
                     /locus_tag="ACZPCL_08050"
     CDS             complement(105..1736)
                     /locus_tag="ACZPCL_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HIR1162452.1"
                     /translation="MTIKQIRRWAAAVAAICLLTLFLPLYGMTAFAATARIAFSDPTA
                     SVGSEINVKMKITSSDNLSTADIMLAYDPGSLEFIEGTNAEGGNGAVRVHGDGGGANT
                     TTLAYDLKFKASAAGTSKITVTSQEIYDASSQIVTVDKQGESQVTVSALSSASKDASL
                     KSLQVSPGSLTPEFSSDVDSYAVTVGTDVDKLIISADTTDEGASKVVSGNENLQMGEN
                     RVTLKVTAQDGETTKEYVIVVTKQEGGPNESTQDGTGEESFEQFQMTVSRRSFTVMAP
                     DASVQVPEGLVETAITIDGHAVKGWVWGNETEHQYCVIYAMNEAGEKNFYRYDLNNNE
                     RTIQRYFEDPAAQGAVSEEVYTQLADKYDSLRNDYSLFRTLLIAAIVVALIFMVLLIV
                     SVTGKKKKSSSAEKKTARSEKKPVKEEDEDEDDLYGEEVEEPEDEPEDDLEEVDDQDE
                     DDFQFEDLDDADDKEPEEDEPEEDSVDENEKENPKEDTEDEDLEEDDIEDFDFEDLDI
                     DEVEEQEEEKEPAGSEETSEDQNEKEEDDFEVFDL"
     gene            complement(1761..3968)
                     /locus_tag="ACZPCL_08055"
     CDS             complement(1761..3968)
                     /locus_tag="ACZPCL_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR1162453.1"
                     /translation="MKGKIYKKGLAVLLGLTLAANLPVTSPLSIHMLDSYAYTGAATV
                     KASSLNVRSDAGTGYSAVARLAAGASIQITGEKKGTDGKTWYRIQYTGSNGQAGTGYV
                     SAAYIRLPVSYSTDASFESYLTSQGFPESYKDGLRQLHAQYPNWVFRAKNTGIDWNTA
                     IENESVLGRNLVATGSISSWKSVESGAYNWDNSTWTGFDGSNWVAASQDIIRYYMDPR
                     NFLDDTYVFQFLSHEYDGSTQTKEGLTGMVSGSFLSGTTDSTGTGGSSSSGGSSTPGG
                     SSSSGSSSSSVSQEGPGGRNGATGDTNHGPGVAGGSPSGSSSSGESSTTSGSNDVSTQ
                     APGSSSGSGNSTESSTGDGNVSLEAPHASITPKEHNLVTSNVSLVAPGQSDGTINSTG
                     SSQPGTVTTDNSSSSPSGQTTSPSSGSSGSKSYVDILMDAAAQSGVSPYVLAAMILQE
                     QGTNGGTPLISGNYSGYSGYYNFFNVEAYQSGSMSATQMGLRYASQSGSYGRPWNTVE
                     KSILGGAQNYGDNYVKAGQNTFYLKKFNVQGSNPYKHQYMSNVQGAASEAERLSKAYS
                     SLKSSALEFQIPVFNNMPAQACSAPTGDGSPNNKLSGLSADGFSLTPSFSKDTTSYNL
                     IVDTSVTSLQISATAADGKASVAGTGNTELTGNSSDITITVTAQNGSTRTYSIHVVRQ
                     SGGNTPSGTGSANSSNSSSSSSPASPGGSSGGPGDTGSSQTPGGSNVTIVNVQ"
     gene            complement(4250..5494)
                     /locus_tag="ACZPCL_08060"
     CDS             complement(4250..5494)
                     /locus_tag="ACZPCL_08060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1162454.1"
                     /translation="MRKKIRPVWLWLLFLTLFLGLIFQSFPVKAQEQEELRLYSQSVV
                     LMDGLSGRILYGKNAEQIRPMASTTKIMTCILALENGKTEEWVSASSYAASQPKVRMG
                     VRKGEQYRLKDLLYGLMLESYNDAAVMIAEFIGGTREGFAAMMNEKARELGCKDTWFI
                     TPNGLDAQETDEKGEIHTHSTTAKDLARILLYCIQESPKKEEFLKITRTQNHGFYDLE
                     NKRQIQCRNHNALLDMMDGALTGKTGFTGGAGYSYVGALEKNGKLFIIALLGCGWPPH
                     KTWKWSDARSLLSYGMENYEKTDLYERCQKTEPLPVKVDGGICWGEQPQDHVLAREDL
                     RSEEKHVYALVKKEEKQKKKVFLPKQLQAPVKEGQKIGRVCYYVEGELLREIPLYSDR
                     QITKMTFWGTLEHIFGLWVHKC"
     gene            5682..6617
                     /locus_tag="ACZPCL_08065"
     CDS             5682..6617
                     /locus_tag="ACZPCL_08065"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1162455.1"
                     /translation="MIKKTLQYKITEKDQGKTVEQFLRSKGYSHRLVVKLRNSPTGLT
                     IGGELAFTIHRLQAGEILIATLEEENSQNIVPVELPLNIVYEDEDILVVNKDAGVPIH
                     PSQGHFDHTLANAVAWYYQSKGQTLVYRAINRLDRDTTGLLVIAKHLLAGCILSDQML
                     NRQIHRQYRAIVCGKTEKEGIIDAPIGRAEDSTVLRRIDFEHGETARTRYRLLSYDEE
                     KDLSYISLKLDTGRTHQIRVHMSYIGHPLPGDFLYNPDYRYISRQPLHSYGLEFIHPL
                     TGEPLSFTAHLPADMAALVKEPELTSTEGLWDNDF"
     gene            complement(6580..8091)
                     /locus_tag="ACZPCL_08070"
     CDS             complement(6580..8091)
                     /locus_tag="ACZPCL_08070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HIR1162456.1"
                     /translation="MTELPQRFKERMRELLGNEYEAFEASYEQEKVQGLRFNSLKTKE
                     GREPDWEEAGVKTLAAETAKLLNMELTPVPWVKEGYYYPLDARPGKHPFHEAGLYYIQ
                     EPSAMSVVEFLNPKPGENVLDLCAAPGGKSSHIASRLKGEGFLLSNEIHPGRAKILSQ
                     NMERMGVGNCVVTNEDAASLAKVFPQFFDRIAVDAPCSGEGMFRKEEEAVRQWSEDHV
                     KMCAARQREILEDAASMLKPGGTMVYSTCTFAPEENEGTILAFLKSHDDFYLEERECP
                     KGLMAAVPQWAFFGADKEDDSERDLAGENGIEKYHLERAFRIMPHKTEGEGHFMAVLR
                     RKEDGMGFSGKRSLPAYMDLKKEKDVLKELHRFLEETLTEPEVLKKRKEYLRFGDQLY
                     LLPPQMVSLKGLKVLRPGLHIGTIKKNRFEPSHALALWLFPEDAKVQKEVEPDGTEAV
                     KYLKGETLTGDTGMSAPCEKGWVLICTGNVSLGWGKMAAGTIKNHYPKGLRWM"
     gene            complement(8215..8625)
                     /locus_tag="ACZPCL_08075"
     CDS             complement(8215..8625)
                     /locus_tag="ACZPCL_08075"
                     /inference="COORDINATES: protein motif:HMM:NF040112.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6465 family protein"
                     /protein_id="HIR1162457.1"
                     /translation="MTMAKKAAKTSAVKENVKETAKEVAPVVKETVKEAAATVKAVAA
                     EAKEAVKKAPAKKPAAKRTTAKKAVEPKMEVHFQFAGKDILAKSVLDQAVEAYKSTHK
                     DVEIKKVELYIVAEEGAAYYVVNGEASDDFKVML"
     gene            complement(8768..10651)
                     /locus_tag="ACZPCL_08080"
     CDS             complement(8768..10651)
                     /locus_tag="ACZPCL_08080"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR1162458.1"
                     /translation="MKDMEKKEKKIPVSRSKSAFKERERERKEKERAEREKAKAVMEE
                     NLADENETQENETQETEKKPFSWKLAAGILAGVVITGAAGYVGMSMKYQNTYLPGTCI
                     NGMNAAGMSPKAVEEAMAKEAGDYSLRLVLRNDASENIEGSAIGLNTVFDGSLENILK
                     QQNPYTWPIHMIKGENYEIETMLAYEDEALDQAVDSLNAMDPAHVTAPENAHLSDYIK
                     GEGYSVIPETEGDQLNPEKVKEEVKAAINDLKEEIDLDELGCYEEPAVRSDDESLTAM
                     ADSLNHYVNMTVTYTMGSKNEILDGEQIHTWLSYSDGQVTVDEGKISEYVKSLASKYN
                     TAYTKRTFKTSYGPEVEVSGVYGWRIDQQAEAAALKEALAEGKNVTKEPAYAQKAASH
                     DGNDYGNTYAEVNLTAQHMYFYKDGKKILESDFVSGNVSKGYTTPPGLFSLTYKQRDA
                     TLKGQGYASPVKFWMPFNGGIGFHDASWRNTFGGTIYKKNGSHGCVNMPYAAAKTLFE
                     NVYAGMPVICYNLAGTENAQSSKASGKDDTAVPAQPTTAAQPTQAPSQTQPAETQAPA
                     TDPAGPGETTKAPETAAPTQAPSESAIVQPIETTSPAENATKEVGPAFTTEAPAEEIG
                     PGV"
BASE COUNT         2459 a         2771 c         2170 g         3354 t
ORIGIN      
        1 ttactttttt tacacaatac atcgcttatc atcctttctg ttcctatttg tcaggcagcc
       61 cattcctgac tttcagggcc gcctggttgt ttaagcgtta cattttacag gtcaaatacc
      121 tcaaaatcat cttcttcttt ttcgttctgg tcttcagaag tttcttcgga tccggcaggt
      181 tctttttctt cttcctgctc ttccacctcg tctatatcca gatcttcaaa gtcaaagtct
      241 tctatatcat cttcttccag atcttcatcc tctgtatcct cttttggatt ttctttttca
      301 ttttcatcta cagagtcttc ctctggctcg tcctcttccg gttccttatc atctgcatca
      361 tccagatcct caaactggaa gtcatcctcg tcctggtcgt ctacttcttc cagatcatct
      421 tccggttcgt cttcaggctc ttccacctct tcgccataaa gatcgtcctc gtcctcatct
      481 tcctctttta caggtttctt ttcagaacgg gctgtctttt tctctgctga agaagatttc
      541 tttttcttac ctgtaacaga tacgatgaga agtaccataa agatcagtgc tacaacgatg
      601 gcagcgatca gcagtgtcct gaacaggctg tagtcatttc ttaaggagtc atacttgtct
      661 gccagctgtg tataaacttc ctcacttact gctccctgag cagccgggtc ttcaaagtaa
      721 cgctgaatgg tacgctcatt attatttaag tcatagcggt agaaattctt ctcgccagct
      781 tcattcatag cgtagatcac gcaatactga tgctctgttt cattgcccca tacccagcct
      841 tttacagcat gtccgtcaat ggtaatggct gtttccacca gtccttccgg tacctgtaca
      901 gaagcatccg gtgccatcac tgtaaagctg cgtctggata cagtcatctg gaactgttca
      961 aagctttctt caccagttcc atcctgtgta ctttcatttg gaccgccttc ctgcttggtg
     1021 acaacgatca catattcctt tgtagtttct ccgtcctggg cagttacttt taaagtaact
     1081 ctgttttcac ccatctggag attttcattg ccgcttacca ccttggaagc gccttcatct
     1141 gtagtatctg cactgatgat cagtttatcc acatcggttc ctacggtaac tgcataggag
     1201 tctacatcag aagaaaattc cggtgtcagg cttcccgggg atacctgaag ggattttaag
     1261 cttgcgtcct tggaagcgct tgataaagca ctgactgtta cctgggactc accttgtttg
     1321 tctacggtta cgatctggga agacgcatcg tagatctcct ggctggtaac tgtaatcttg
     1381 ctggttcctg ctgcagatgc cttaaacttt aagtcatagg caagggtggt ggtatttgcg
     1441 ccaccgccgt caccatgaac acggactgct ccatttccgc cttctgcatt ggttccttct
     1501 atgaattcca ggcttcccgg atcatatgcc agcatgatat ccgctgtaga aaggttatca
     1561 ctgctggtga tcttcatttt tacattgatc tcactgccta cagatgcggt aggatctgaa
     1621 aaagcgatcc ttgcagtagc tgcaaacgcc gtcattccgt ataatggcaa aaataaggtt
     1681 aatagacaga tggctgcaac tgcggctgcc caccgtctga tctgtttgat tgtcatattc
     1741 attcctgctt tcctgttttt ttactgcaca ttcacaatgg tcacattgct gcctccaggt
     1801 gtctggctgc ttcccgtgtc acctggtcct ccactgcttc cgcccggact tgccggactg
     1861 gaagaggaac tgctgtttga agaatttgct gatcccgtgc cggaaggtgt gtttcctcct
     1921 gactgacgta ctacgtggat actgtatgtc cttgtgcttc cattctgagc tgtaacagta
     1981 atagtaatat cactggaatt accagtaagt tctgtatttc cggtacctgc aacggatgcc
     2041 ttgccgtctg ctgctgtagc gctgatctga aggctggtca cagaagtatc tacgatcaga
     2101 ttgtagcttg ttgtatcctt gctgaaggaa ggagttaagc tgaaaccatc tgcactcaga
     2161 ccagaaagtt tgttgttagg acttccgtct cctgtaggcg cgctgcatgc ctgtgccggc
     2221 atgttgttaa atacagggat ctggaattcc agggcagaac tctttaagga actgtatgcc
     2281 ttggaaaggc gctctgcttc tgaggctgct ccctgtacat tgctcatgta ctgatgttta
     2341 taaggattgg atccctgcac attgaacttt ttaaggtaaa atgtattctg ccctgctttt
     2401 acatagttgt caccataatt ctgggcaccg cctaaaatgg atttttctac tgtattccat
     2461 ggtcttccat aactgccgga ctgacttgca taccgcagtc ccatctgggt tgcagacatg
     2521 gaaccggact gataggcttc tacgttaaag aagttatagt aaccggaata tccggaataa
     2581 ttacctgaga tcagcggtgt tccgccattg gttccctgct cctgaaggat catggcagct
     2641 aaaacataag gacttacgcc ggactgcgct gctgcatcca taaggatatc cacatagctc
     2701 ttgcttccag atgagccgga agaaggactt gttgtctggc cggaaggact ggaagaactg
     2761 ttatcagtgg ttacagttcc aggctgtgaa ctgcctgtgc tatttatcgt gccatcagac
     2821 tgtcccggag ctaccaggct tacattggaa gttaccaggt tatgctcctt tggtgtaata
     2881 gacgcatgag gcgcttccag acttacattt ccatctcctg tactgctttc tgtactgttt
     2941 cccgaaccgg aactgcttcc cggtgcctgg gtgcttacat cattgctgcc ggatgtagtg
     3001 ctgctctcac cagaagaact gctgccggaa gggctgcctc ctgccacacc aggtccatga
     3061 tttgtatcac ctgttgcacc gtttcttcct cccggtccct cctgtgaaac agaactggaa
     3121 gaactgccgg aagagctgct gcctcctggt gtagaagaac cgccggaaga agaacttccc
     3181 cctgttccgg tggagtctgt tgttccggaa aggaaggagc cggaaaccat tcctgtaagg
     3241 ccctcttttg tctgtgtgct tccatcatat tcatgggaaa ggaactggaa tacataggta
     3301 tcatctaaaa agtttcttgg gtccatgtaa taacggataa tgtcctggga agctgctacc
     3361 cagttgctgc cgtcaaagcc ggtccaggta ctgttatccc agttataggc accgctttct
     3421 acggatttcc aggaagaaat gcttcctgtt gcaaccaggt ttcgacccag gaccgactca
     3481 ttttcaatgg ccgtattcca gtcgatccct gtatttttcg cccggaatac ccagtttgga
     3541 tactgggcat gaagctgtct taaaccatcc ttataacttt ccggaaatcc ctgggaagtc
     3601 agataggact caaagcttgc atctgtagaa taggaaacag ggagtcttat gtaagcggcg
     3661 gatacataac ctgtacctgc ctgaccgtta ctgcctgtgt actggatccg gtaccaggtc
     3721 tttccatcag ttcccttttt ttctcctgtg atctggatgc tggctcctgc tgccagacgg
     3781 gccactgcac tgtaaccggt tcctgcatca cttcttacat ttaaagagga cgccttgact
     3841 gtagccgccc ccgtataagc ataggaatcc aacatgtgga tggacaacgg gctggtcacc
     3901 ggaaggttgg ccgccagtgt caggcctaac aggactgcca gacctttttt gtatattttt
     3961 cccttcatct tgctgtatta tctcctgaat tttctgtatc tcatgatacc agattgctac
     4021 gcactttgtg ctctcgcaat cttcaaaacc attagtaatc cgctcatttt ttgcaaaaat
     4081 ggctacttac tcatgttttt gcgtgtccca agataaaaaa tcctcctgca agcaggcttg
     4141 cgtcggattt ttacctttcg acactggtat cattataatg atatttactc cttaatgtca
     4201 accgcaacaa aaaatgttca cacaaatcaa actaattcgt aagaaaactt taacacttgt
     4261 ggacccacag accaaaaata tgttccagcg ttccccaaaa agtcattttt gtgatctggc
     4321 ggtcactgta taagggaatt tcccttagca attccccttc tacataataa caaacccgtc
     4381 ctatcttctg cccttctttt acaggagcct gcagctgctt tggcaggaac accttttttt
     4441 tctgtttttc ctcttttttt accagagcat aaacatgttt ttcctctgat cgtaaatctt
     4501 cccttgccag cacatggtcc tgaggctgct ctccccagca gatgccgcca tctactttta
     4561 caggcaatgg ctctgttttt tggcagcgtt catacagatc tgttttctca taattttcca
     4621 ttccatagga aagaaggctt ctggcatctg accatttcca ggttttatga ggaggccagc
     4681 cacagcctaa cagggcaatg ataaaaagtt ttccattttt ttccagcgca ccaacataag
     4741 aataacctgc gcccccagta aaaccggtct ttcctgtaag ggccccgtcc atcatatcca
     4801 aaagagcatt gtgattgcgg cactggatct gccttttatt ttccagatca taaaatccat
     4861 ggttctgggt cctggttatc tttaaaaact cctctttttt cggcgactcc tgtatacagt
     4921 ataataagat ccttgccaga tcttttgctg tagtggaatg ggtatgtatt tctccctttt
     4981 catctgtttc ctgggcgtct aaaccattgg gggtaataaa ccaggtatcc ttacagccta
     5041 actccctggc tttttcattc atcatagctg caaaaccttc tcttgtaccg cctataaatt
     5101 ccgcgatcat cacagctgca tcattataag actccagcat caggccatac agcaggtcct
     5161 ttaaacggta ctgctcccct tttcttactc ccatacggac tttaggctgg gaggctgcat
     5221 aagaagaggc acttacccat tcttccgttt ttccattttc aagggcaagg atacaggtca
     5281 tgatcttcgt ggtgctggcc atgggacgga tctgttctgc attttttcca tataagatgc
     5341 gtccgcttaa accatccata agcacgacgg actgggaata caggcggagt tcttcctgtt
     5401 cctgggcctt aacaggaaaa ctttgaaata tcagccccag aaacagtgtt aaaaacagaa
     5461 gccataacca tacaggcctg atcttttttc tcatccattt tctccctgga gcatttatgt
     5521 actctcaaaa tcttatttca tcctattccc attttcctgc agatatgcaa taacctgcac
     5581 cggaagcttt tgacgaacct tttaggctgt gttatgattt acaggaaact taaaacagat
     5641 aaacgatcta acaaagatca tgaagggaat acagacaatc gatgatcaaa aagacattac
     5701 aatataaaat aacagaaaag gaccagggaa agaccgtaga acagttttta agaagcaaag
     5761 gttattccca ccgtctggtg gtaaaactgc ggaattctcc taccggcctt accatcgggg
     5821 gagagcttgc atttaccatc cacaggctcc aggcaggtga gatacttatt gctactttag
     5881 aggaggaaaa ttctcagaat attgtccctg tagaacttcc tttaaatatt gtttacgaag
     5941 atgaagatat tttagtggta aataaagatg ccggtgtacc catccaccct tcccagggcc
     6001 attttgacca cacgctggca aatgcagttg cctggtatta ccagagcaaa ggccagacct
     6061 tagtctaccg ggcgatcaac cgcttagacc gggataccac cggcctttta gtgatcgcta
     6121 aacatctttt agctggctgc atcctttcag accagatgct taacaggcag atccacagac
     6181 agtaccgggc aattgtctgc ggaaaaacag aaaaagaggg catcattgat gcccccattg
     6241 gacgggctga agactctacc gtccttcgcc gtattgactt tgaacatggt gagacagcca
     6301 ggacccgtta ccgcctgctc tcttatgatg aagaaaagga tctttcctat atcagcctta
     6361 aattagatac ggggcggacc caccagatcc gtgtccatat gagttatatt ggccaccccc
     6421 ttcccggaga tttcctctat aacccggact accgttatat cagccgccag ccccttcact
     6481 cttatggtct ggagtttatc catcccctta ccggagagcc tttaagcttt acggcccatc
     6541 tgcctgcgga tatggcagct ttggtaaaag agccggaact tacatccacc gaaggccttt
     6601 gggataatga tttttaatgg tcccggcagc catcttgccc cagccaagac ttacatttcc
     6661 tgtacagatc aatacccagc ctttttcaca gggagcagac ataccggtat caccggtcag
     6721 tgtctccccc tttaaatatt tcactgcctc tgttccatct ggttctactt ctttttgtac
     6781 ttttgcatct tccggaaaca gccacaatgc cagggcatga gatggttcaa agcggttctt
     6841 tttaatggtt ccaatatgaa gccccggacg cagcactttc agtcctttta aacttaccat
     6901 ctgaggcggc agcagataca gctggtcccc aaaacgcaga tattcttttc tttttttcag
     6961 tacttccggc tctgtcagtg tctcctccag aaaacggtgc agttctttta acacatcttt
     7021 ttcttttttc agatccatat aagcaggcag gcttcttttt cctgaaaagc ccataccgtc
     7081 ttctttgcgg cggagcactg ccataaaatg gccttctccc tctgtcttat ggggcataat
     7141 gcggaacgca cgttccagat gatacttttc aattccgttc tctcctgcca ggtcccgttc
     7201 agagtcatct tccttatctg ctccaaaaaa ggcccactgc ggcactgcag ccataagccc
     7261 tttggggcat tctctctctt ccaggtaaaa atcatcatgg ctctttaaaa aggcaagaat
     7321 ggtcccttca ttttcttccg gcgcaaaagt acaggtagaa tagaccatgg ttccccctgg
     7381 ttttaacata gaagctgcat cttctaagat ctctctctga cgggcagcgc acatttttac
     7441 atggtcctcg ctccactgtc tgactgcttc ttcctcttta cggaacattc cctctccgga
     7501 acagggagca tctactgcaa tccggtcaaa aaactgagga aagactttcg caaggcttgc
     7561 tgcatcttca tttgtgacta cgcagtttcc aactcccatg cgctccatat tctgggaaag
     7621 tatctttgcc ctgcccggat ggatctcatt tgataaaaga aagccttctc cttttaaacg
     7681 ggatgcaata tggctggact ttccgccggg agctgcacac agatccaata cattttcccc
     7741 tggtttcgga tttaaaaact ctactacgga catggcactt ggctcctgga tatagtaaag
     7801 tcctgcttca tggaacgggt gtttgccagg tcttgcatct aacgggtaat aataaccttc
     7861 ttttacccag ggaaccggcg ttaattccat atttaagagt tttgcggttt ctgctgccag
     7921 agtctttacc cctgcttctt cccagtcagg ttctcttccc tcttttgtct ttaaactgtt
     7981 aaaccgcagt ccctgtacct tttcctgctc atagctggct tcaaatgctt catattcatt
     8041 tccaagcagt tccctcatgc gctctttaaa gcgttgtggc agttctgtca tcccattgtt
     8101 tcctttcttt gtcaaattac gagtttatca gattatgtta caaacaggaa aaggccggca
     8161 ggtctatccg cccaccagcc tttttcataa tttctgttct aaaagatttt tcgattacag
     8221 cataaccttg aagtcgtcag atgcttcacc gtttactaca tagtaagctg cgccctcttc
     8281 tgcaactatg taaagctcaa ctttcttgat ctctacatct ttgtgagtgc tcttgtatgc
     8341 ttctacagcc tgatctaata cgctcttagc aaggatatcc ttaccagcga actggaaatg
     8401 tacttccatc tttggctcaa ctgccttctt tgcagttgtt ctcttagctg caggcttctt
     8461 tgcaggagcc ttcttaacag cttcctttgc ctctgctgca actgccttta cagtagctgc
     8521 tgcttctttt acagtctcct ttactactgg agctacctct tttgcggttt cttttacatt
     8581 ttcttttact gcagatgttt tagctgcctt cttagccatt gtcataattc attcctccct
     8641 ggttggatct gggatatccg gagtccaact ccgaaatccg tctttatttc gttctatgta
     8701 tgttcgtcct cgttgtgtca aaacgcatta tagaatggtt tttgcgattt gtcaacgttt
     8761 tctgccatca gacccctggt ccgatctcct ctgcaggagc ttctgtggta aacgcaggcc
     8821 ccacttcttt tgtggcattc tccgccggag aggtggtttc tatcggctgt acaatagcag
     8881 actcggaagg tgcctgggtc ggtgcagcag tctctggtgc ctttgtggtc tctcccggtc
     8941 ccgcagggtc tgtagcaggt gcctgagtct ctgccggctg ggtctgggac ggtgcctggg
     9001 tcggctgtgc tgccgttgtt ggctgtgcag gtactgctgt gtcatccttt cctgaagcct
     9061 tggaagactg ggcattttcc gttcctgcca ggttataaca gatgaccggc ataccggcat
     9121 acacattttc aaataaagtt ttggctgctg catatggcat atttacacag ccatgggagc
     9181 cattcttttt gtaaatagtg ccgccaaagg tatttctcca gctggcatca tggaaaccga
     9241 taccgccgtt aaatggcatc cagaacttta caggacttgc atacccctgt ccctttaagg
     9301 ttgcatccct ctgcttgtaa gtcaggctga acagtcccgg aggcgtggta tatccctttg
     9361 aaacatttcc ggatacaaaa tcagactcca ggatcttctt tccatctttg taaaaataca
     9421 tatgctgggc cgttaaattt acctctgcat aggtatttcc ataatcattg ccatcatggc
     9481 tggccgcttt ctgggcatag gcaggttcct ttgtaacatt ttttccctct gcaagggctt
     9541 cttttaatgc agctgcctct gcctgctggt caatacgcca gccataaaca ccgcttacct
     9601 ctacttccgg tccataactg gtcttaaagg tacgttttgt atatgcagta ttgtatttgg
     9661 atgccaggga ctttacatat tcagagatct ttccttcatc cactgtgacc tgaccatctg
     9721 aataagaaag ccaggtatgg atctgctcac cgtccaggat ctcattcttg ctccccatgg
     9781 tataagtgac tgtcatattt acataatggt tcaggctgtc agccatagct gtaaggcttt
     9841 catcatctga gcggacagca ggctcttcat agcagcccag ctcatccaga tcgatctctt
     9901 ctttcagatc gttaatagcc gcctttactt cttcctttac tttttcagga tttaactggt
     9961 ctccttctgt ctcaggaata acagaatagc cttctccttt tatatagtct gataaatgag
    10021 cattctccgg cgcagtcaca tgagccggat ccatggcatt taagctgtct actgcctgat
    10081 caagagcctc atcctcataa gccagcatag tctcgatctc ataattttcc ccttttatca
    10141 tatgaatggg ccaggtataa ggattttgct gcttaaggat gttttcaaga cttccatcaa
    10201 atacagtatt aagcccaatg gcacttccct ctatattctc agacgcatca tttctcaaca
    10261 ccaggcgcag ggaataatcg cctgcctcct ttgccatagc ttcttctact gctttaggac
    10321 tcattcctgc tgcattcatt ccattaatgc aggtacctgg aaggtatgta ttctggtatt
    10381 tcatagacat acccacatat ccggctgcac cggttattac tacacctgcc agtattcccg
    10441 ctgcaagctt ccaggaaaat ggctttttct ctgtttcctg tgtttcattt tcctgtgttt
    10501 cattttcatc tgccaggttt tcttccatga cagccttggc cttttctctt tccgctcttt
    10561 ctttttcttt tctttcccgc tccctttctt taaaagccga cttactgcgg cttacaggaa
    10621 tctttttttc ttttttttcc atatctttta tataaaaaac ctttctgaca ttgtcatttt
    10681 tcattcatga atgctttcag gatatctgtc agctccttta ggctgtccgt gatatttcta
    10741 aaggctccat aatt
//
LOCUS       DBMDUA010000091        10749 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3031_length_10749_cov_3.177780, whole genome shotgun sequence.
ACCESSION   DBMDUA010000091 DBMDUA010000000
VERSION     DBMDUA010000091.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10749)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10749)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10749
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3031_length_10749_cov_3.177780"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2704
                     /locus_tag="ACZPCL_08085"
     CDS             <1..2704
                     /locus_tag="ACZPCL_08085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005345319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR1162459.1"
                     /translation="LKKVSDESEIRDYIDKAQEEAGFLYFYFLSADGNYKMLTGEAGY
                     LGLQENLEDKITLGEDIITNAVVPGKQQMLVFASPQSHGSYQGFEYDAIAIAYENADI
                     VDVLDISAFNGNAKSYVVHPDGRVVIDHSFEAWGTVYNFFGVLREHSNISEKEILELS
                     EKFKERRTDTMLVNLDGSNYYLVYGSSECQDWIFLGLVQADIVNASMNSLQLRTMLLG
                     GTIVFGFAVFIIELILQKNRISLKRRDVEILYRDELFQKLSMSVDDVFLMLDAKTYKA
                     DYVSPNVEKLLGITVEQIQKDISVLGKLHSEDCEDPKKNYLKEIQVNEQQEWDFEYVH
                     QKTGEQRWFHIIAMGSEVNGKKKYILVMSDRTVDKKMNQALYEAVRAAETANRAKSTF
                     LSNMSHDIRTPMNAVIGFTTLAVSNIDNKEKVRDYLGKILSSSNHLLSLINDVLDMSR
                     IESGKIHLEETEVSLSDMLHDLKTIISGHIYAKQLELYMDAMDVTDEDVYCDKTRLNQ
                     VLLNLLSNAIKFTPAGGTVSVRLKQFPGTKKGSGLYEIRVKDSGIGMSQEFIQRIFSP
                     FERERTSTVSRTQGTGLGMAITKNIVDMMGGTIEVRTEQGKGTEFIVRLPLRIQSEQR
                     SIEKIAELEDLKALVVDDDFNTCDSVTKMLVKVGMRSEWTLSGKEAVLRARQSMELGD
                     AFHAYIIDWRLPDMNGIEVTRQIRSLGDDTPIIILTAYDWSDIEVEARAAGVNAFCAK
                     PLFMSDIRETLMTAIGQKQTGAENCILPETGSDFRGRCILLVEDNELNREIAEEILKQ
                     YGFLVDIAENGAVAVEKVKNSAPGTYDLVLMDIQMPVMNGYEATEQIRALEDPALAKI
                     RILAMTANAFDEDRKQALKCGMDGFLSKPIVMEELIRTLQNNMM"
     gene            complement(3087..5375)
                     /locus_tag="ACZPCL_08090"
     CDS             complement(3087..5375)
                     /locus_tag="ACZPCL_08090"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR1162460.1"
                     /translation="MVEHTMTKETLKNRLTALCGKEISACSEKELYTGLLSLVQEMAK
                     DKESNQGKKKLYYISAEFLIGKLLSNNMINLGIYDTVKNLLEENGKSLSAVEETEPEP
                     SLGNGGLGRLAACFLDSIATLGLNGDGVGLNYHFGLFKQLFKDNKQAEEPNPWIENTS
                     WLTKTDVTYKIPYKNFTLTSRMYDIEVTGYNNRTNKLHLFDMETVDESIVEDGISFDK
                     TDIAKNLTLFLYPDDSDKEGRILRIYQQYFMVSNAARLILDEAITKGSTLYDLADYAV
                     IQINDTHPTMVIPELIRLLTTEHGMEMDDAIEIVSHCCAYTNHTILAEALEKWPVEFF
                     KEAVPQLLPIMEVLDDKVRRKFDDPSVYIIDKNDLVHMAHIDIHYGFSVNGVAALHTD
                     ILKETELNNFYKLYPEKFNNKTNGITFRRWLLHSNPELTDFITGFIGDGFKKDAEELK
                     KLDTPVITKNLKQLYRLLDIKAQKKAELAKYLEETQGITINPDSIFDIQIKRLHEYKR
                     QQMNALYLIHKYLEIKKGKKPTTPITAIFGAKAAPAYIIAKDIIHMILCLQQIIDKDP
                     EVKPYLNVVMVNNYNVTLAEKLIPACDISEQISLASKEASGTGNMKFMLNGAVTLGTD
                     DGANVEIHELVGDDNIYIFGDSSETVIERYKKANYCSKDYYDADPAIKEAVDFIISKK
                     MLAVGDKENLERLYKELLTKDWFMTFPDFKSYCEAKEKAFADYEDRTLWARKMLVNIS
                     QAGYFSSDRTIGEYDRDIWKLR"
     gene            5592..6494
                     /locus_tag="ACZPCL_08095"
     CDS             5592..6494
                     /locus_tag="ACZPCL_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1162461.1"
                     /translation="MNRGYHEIKEHGKREFPFNIYPCSIPVDFSHVALHWHEEMEIIA
                     VKKGKGRITVDTVSYPVEEGDILAVFPGQLHAISCGNGERMEYENIIFRLSMLMGDNG
                     DGCTVHFLKPLQEGQAKNPVLIQRNQPDHEVFGKVIQELDRFSEIRGNGYQLAVKGQL
                     FLFLYYAYGRGILFTEKKPGHAREKIKGILEYIREHFGEEITIRQAAELCFYSESHFM
                     KYFKQYAGMPFIQYLNDYRLTRAGEYLKMTEENVTQIALKCGFENLSYFNRLFRRKFG
                     VTPRQYREEQEAAAGVSGESSPLL"
     gene            6599..8104
                     /gene="malQ"
                     /locus_tag="ACZPCL_08100"
     CDS             6599..8104
                     /gene="malQ"
                     /locus_tag="ACZPCL_08100"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR1162462.1"
                     /translation="MRECGMLLPIASLPSKYGIGCFSKEAYKFVDQLKEAGQSFWQIL
                     PLGPTGFGDSPYQAFSAFAGNPYFIDLEQLVGEGLLTEDECEEADFGDDDRYIAYDKI
                     YKARFKVLKKAYERWKKDHHQFLKSKLDPETIEYCFYMAVKDHLKSASWNKWDKDIRL
                     RKPAAVEKYKKMLADEIGFYEFQQFEFNEQWSRLKAYANENGIRIIGDIPIYVAFDGA
                     DSWAHPELFQFDEECLPKAVAGCPPDAFSATGQLWGNPLYDWGYHKKDHYSWWIKRME
                     YSLKLYDVVRVDHFRGFEAYYSIPYGDPTAEFGHWEKGPGLDLFARLKKSLKKEELPI
                     IAEDLGFLTPEVHEMLKESGFPGMKVLEFAFDDSENSDYLPHKYQENCIVYTGTHDNE
                     TLVGWYENMNRRNKSFAREYMGCRHTPKKEMHWEFIRLAMASPAKLAVIPVQDYLGLG
                     AEARINEPSTLGKNWKWRLLPGEITGDVLKQCRRMAKLYGRLPETVSDINM"
     gene            8224..9711
                     /locus_tag="ACZPCL_08105"
     CDS             8224..9711
                     /locus_tag="ACZPCL_08105"
                     /inference="COORDINATES: protein motif:HMM:NF040203.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR1162463.1"
                     /translation="MRDQKRWMKNWIRALAVGTMITAAVPISVMAAEKGWVNEDGAAH
                     YVDSNGSYVKNQWKTSQGVSYYLDENGDLAKNVWVDGTYYAGEDGSMTKDAWIYVDEN
                     DGSMKSGWYYLNRNGKVEKNDWKTIGKGKYCFDSDGRMRTGWHYENGDIYYLGNGSEG
                     YTRSGWYFLESDGKKRPAEGSVSKDLSGESENGRWYYFQSNGKARKGENGAPKETTID
                     GKKYYFDENGVMLTGWQCVKEKAEPGDGTGISRFVYLGGKEKGMLKGQWFETTEKPWD
                     TKAWSTALNTQAVRKNNTDETALMKKDGSRWFYLENDGTPLFLSADATGLKDGAVKLD
                     GRYYFFDSYGVCQSGMIKFTSGGKTETAYFDPEAGGALSIGRNTNVVDKRDKVYTFCS
                     GTSGSEKGCGINGVKDGFLYYNGLLVSAKEGSSYEPFKVSGQVYLVNEAGKVQTEEKK
                     YSSDGNAAYTIENGKVFTLDENGEKKSAATGLSLPYAGWKIQYQR"
     gene            10255..>10749
                     /locus_tag="ACZPCL_08110"
     CDS             10255..>10749
                     /locus_tag="ACZPCL_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1162464.1"
                     /translation="MEQELNRKTMVIGHRNPDTDSICSAICYANLKQAVTGEEYMPAR
                     AGHVNGETQFVLDYFGAEEPQLVEDVRTQVRDIEIRKTKGVADNISLKRAWNIMQENN
                     VVTIPSVREDGTLEGLITVGDITKTYMNICDSSILSKANTQYSNIIETLEAELIIGSA
                     EAYFD"
BASE COUNT         3152 a         1915 c         2736 g         2946 t
ORIGIN      
        1 tctgaagaag gtttctgatg aaagcgagat ccgtgattat atagataaag cccaggaaga
       61 ggccggtttt ttatatttct attttctgtc agctgacggc aactataaga tgttaacagg
      121 tgaagccggg tatcttggat tacaggaaaa tcttgaagat aagatcacgc tgggggagga
      181 tatcataaca aatgcagtag ttcccggaaa acaacagatg cttgtatttg ccagccccca
      241 gtctcatgga agctatcagg gttttgaata tgatgcaatt gccattgcat atgaaaacgc
      301 tgatatagta gatgtattgg atatctctgc ttttaatgga aatgccaaga gttatgtggt
      361 tcatccagat ggccgtgttg tcatcgatca ttcctttgag gcatggggaa ccgtgtataa
      421 tttctttggt gttttaagag agcattctaa tatttccgaa aaggagatcc ttgaactttc
      481 agagaaattt aaagagagac gtacagatac gatgctggta aatctggatg gaagtaatta
      541 ctatctggtt tatggaagct cggaatgcca ggactggata tttctgggac ttgtccaggc
      601 tgatatagtc aatgccagca tgaattccct gcagcttcgg actatgctcc ttggaggaac
      661 catagtgttc ggttttgctg tctttattat tgaactgatc ctccaaaaga acaggataag
      721 cctgaaaaga agggatgttg agatcctcta tagggatgag ctgtttcaaa agctgtccat
      781 gagtgtagat gacgtttttc tgatgctgga tgcaaaaaca tataaggcag attatgtaag
      841 tcccaatgtg gaaaaactgc tgggcattac agtagaacag atccaaaagg atattagcgt
      901 cttgggaaaa ctgcattccg aagattgtga agatccaaag aagaattatc tgaaagagat
      961 ccaggtaaat gagcagcagg aatgggattt tgaatatgtt caccagaaaa caggggaaca
     1021 acgctggttt catattattg caatgggcag tgaggtaaac ggaaaaaaga aatatatcct
     1081 ggttatgtct gaccgtaccg ttgataagaa aatgaaccag gccctttatg aggcagtccg
     1141 cgctgcggaa actgccaacc gggcaaagag tacattcctt tccaacatgt cccatgatat
     1201 ccgtacgcca atgaatgctg ttatcggttt tactacactg gctgtaagca acattgataa
     1261 taaggaaaaa gtcagggatt atctgggaaa gatcctttca tccagcaacc atctgctttc
     1321 actgatcaat gatgtactgg atatgagccg tattgagagc ggaaagatcc atctggaaga
     1381 aaccgaggtc agtctgtctg atatgcttca tgatctgaag acgatcatca gtggacatat
     1441 ctatgcgaag cagctggagc tttatatgga tgctatggat gttacagatg aggatgttta
     1501 ttgtgataaa accagactga accaggtgct gctgaatctt ttgtctaatg cgatcaaatt
     1561 tacgcctgca gggggaaccg tttctgtccg gctgaagcag tttccaggga cgaaaaaggg
     1621 cagtgggctg tatgagatcc gggtaaagga tagcgggatc ggtatgagcc aggaatttat
     1681 acagaggatc ttttctcctt ttgaacggga gcggacttcc acagtcagca ggactcaggg
     1741 gacaggactt ggcatggcaa ttaccaagaa tattgtggat atgatggggg gaacgattga
     1801 agttcggaca gagcagggca aaggaactga attcatagtc cgtctgccat tgcggatcca
     1861 gtcagagcag cgcagcatag agaagatcgc agagctggaa gaccttaagg cattggttgt
     1921 agatgatgat tttaatacct gtgacagtgt gacaaagatg cttgtaaagg ttggaatgcg
     1981 ttcagaatgg acactttccg gtaaggaggc cgttcttcgt gcgcggcagt ctatggaact
     2041 gggggatgca ttccatgctt atatcattga ctggcgtctg ccggatatga atggcattga
     2101 agtcacccgt cagatccgca gtctgggcga tgatacgccg atcattatcc tgactgctta
     2161 tgactggtca gatattgaag tagaagccag agcagcagga gtaaatgcat tttgcgcaaa
     2221 accgctgttt atgtcagata tcagagagac actgatgact gctatcggcc agaagcagac
     2281 cggggcagag aactgtatcc ttccggaaac aggttccgat ttcagaggca gatgtatact
     2341 gcttgtggaa gacaatgagt taaaccgtga aattgctgag gagattctta aacaatatgg
     2401 attcctggtt gatatagcgg aaaatggagc agtggcagtg gagaaagtga agaattcagc
     2461 accaggcact tatgatttgg tgcttatgga tattcagatg cctgtaatga acggatatga
     2521 ggccacggag cagatccggg cactggagga tccggcactg gcaaagatca ggatccttgc
     2581 aatgactgcc aatgcttttg acgaagacag aaagcaagca ctgaaatgtg gtatggatgg
     2641 atttttgtcc aagccgattg taatggaaga attgatccgt acattacaaa ataatatgat
     2701 gtaagtgtca aaagtataaa ttgcgtttgt gctcgcacaa ataagcaatt tatacttatt
     2761 gacacgccga acaccgaaaa cgaaataaaa tggtgtgaac acgccatttt atgggagttt
     2821 gaggtgttcg aggattgcgg gaattgcata gcaatggagc aatccggtta catcagtgag
     2881 tggcaaaatt tacttttgac actcacatca taatatgaaa tgagccagat cacagccatt
     2941 tacccatgtt tttggcgggt ctgaagtaaa aaagcctcct gcaaacttgc ttgcaggagg
     3001 tttttctgac cttttgaaca tagtattttt gaacataata ttttttgaac atagtatctt
     3061 tggccctggt atcataaaat ccaggatcat cttaatttcc atatatcccg gtcatactcc
     3121 ccgatcgttc tgtcagagga gaaatatccg gcctggctga tgttgactaa catttttctc
     3181 gcccataagg tgcggtcttc gtagtcggcg aaggcctttt cttttgcttc gcagtaggat
     3241 ttgaagtctg ggaaggtcat gaaccagtct ttggttaaca gttctttgta cagacgttcc
     3301 aggttttctt tgtcgcctac ggctaacatt ttcttgctga tgatgaaatc aacagcttct
     3361 ttgatagccg ggtctgcatc gtagtagtct ttggagcagt agttggcttt cttgtagcgc
     3421 tcgatgactg tttcgctgga atcaccgaaa atgtagatat tgtcatcgcc taccagttcg
     3481 tggatctcta cgttggcgcc gtcatcagtt ccaagggtta cggcaccatt taacatgaat
     3541 ttcatgtttc cggttccgct ggcttctttg gaagcaaggg agatctgctc ggaaatgtcg
     3601 caggccggga tcagtttttc tgccagagtg acattgtaat tgtttaccat aactacattt
     3661 aagtatggtt ttacctcagg atctttgtca atgatctgct gcaggcagag gatcatatgg
     3721 ataatgtcct tggcaatgat gtaggcaggt gctgcctttg ccccgaagat ggcagtgatc
     3781 ggtgtagttg gctttttgcc ttttttgatc tctaaatatt tgtggatgag atacagggcg
     3841 ttcatctgct gtctcttgta ctcatggaga cgtttgatct ggatatcgaa aatggagtcc
     3901 gggttgatag taatgccctg ggtctcttcc agatatttag ccagctctgc ttttttctgt
     3961 gccttaatgt ctaacaggcg gtaaagctgt tttaaatttt tggtgataac tggtgtatct
     4021 aatttcttta attcttctgc atctttttta aatccgtccc caataaaacc ggtgatgaaa
     4081 tcagtcagtt ccggattgct gtgcagcaac cagcggcgga aggtaatacc attggtcttg
     4141 ttattgaact tctcaggata aagcttgtag aagttgttta attctgtctc ttttaaaata
     4201 tcggtatgaa gagctgcaac accgtttacg ctgaaaccat agtggatatc aatgtgggcc
     4261 atgtgtacca ggtcgttttt atcgataata tatacagatg gatcatcgaa tttgcggcgt
     4321 accttatcat ctaaaacttc cataatagga agaagctgtg gaactgcctc tttgaagaat
     4381 tctaccggcc acttttccag tgcttctgcc agtatcgtat ggttggtgta tgcgcagcag
     4441 tggcttacga tctcaatggc atcgtccatt tccataccgt gctcagtagt cagcaggcgg
     4501 atcagttccg ggatgaccat agttggatgg gtgtcgttga tctggatcac ggcatagtca
     4561 gccaggtcat aaagggtaga acctttggta atggcttcgt ctaaaatcag tcttgcggca
     4621 ttgcttacca tgaaatactg ctggtagata cgcaggatgc gtccttcttt gtcgctgtcg
     4681 tctggataga ggaagagagt caggttcttt gcaatgtcgg tcttatcaaa ggaaatgccg
     4741 tcctctacaa tgctttcatc tacagtttcc atatcaaaca ggtggagctt attggtgcgg
     4801 ttgttgtagc cggtaacttc aatatcgtac atacgggatg taagggtgaa gtttttgtat
     4861 ggaattttat aggttacatc tgtttttgtc agccaggaag tattctcgat ccaaggattt
     4921 ggttcttctg cctgtttgtt gtctttaaac agctgtttga aaagaccgaa atggtaattt
     4981 aaaccaactc cgtcaccgtt taagcccaga gtagcaatgg agtctaagaa gcaggcagcc
     5041 agacgtccca gaccgccgtt tccaagagat ggctctggtt ctgtttcttc tactgcactt
     5101 aaggattttc cattttcttc cagaaggttc tttactgtat catagatccc aagattgatc
     5161 atgttattgg aaagcagttt gccgatcaga aactcagcgg agatataata cagttttttc
     5221 tttccctgat tggactcttt gtcctttgcc atttcctgga ccagagataa aagtccggta
     5281 taaagctctt tttcagagca ggcagagatc tctttgccgc aaagggcagt cagtctgttt
     5341 tttaatgttt cttttgtcat tgtatgttca accatgaaat ccatttcctt tctgtggtga
     5401 tatgccaata tctggcccgt ttcattttct gtgtttttat gtgtttatta tagaaaataa
     5461 atctttttcc gtcttgtaga atgccagtat attatagtac aatactatca aaaatgtaaa
     5521 aaatatagaa acatgataaa gaaatggaaa ataaataaaa aaatacagga taacggaagg
     5581 aggagagaaa aatgaaccgg gggtaccatg aaataaagga acatggaaaa agagagtttc
     5641 cgtttaatat ttatccctgc tcgatcccgg tagatttctc ccatgtggct cttcactggc
     5701 atgaagaaat ggaaattatt gctgtgaaga aggggaaggg gcggatcaca gtggatacag
     5761 tgtcttatcc tgtggaggaa ggagacattc tggctgtttt tccgggacaa cttcatgcga
     5821 tctcctgtgg gaatggtgag agaatggagt acgaaaatat cattttccgc ctttctatgc
     5881 tgatggggga taatggagac ggctgtaccg tacatttttt gaaaccgctt caggaagggc
     5941 aggcgaaaaa tccggtcctg atacaaagaa atcagccgga tcatgaagtg tttggaaagg
     6001 tgatacagga gctggaccgt ttttcggaga tacgtggtaa tgggtatcag ctggcggtca
     6061 agggtcagtt atttttgttt ttgtattatg cttatggcag aggcatcttg tttacagaga
     6121 agaagccggg acatgcaaga gagaaaatca aggggatact ggagtacatc cgggaacatt
     6181 ttggggaaga gattacgatc aggcaggcgg cagagctgtg cttttacagt gagtcccact
     6241 ttatgaagta ttttaagcag tatgcgggga tgccatttat ccagtatctc aatgattacc
     6301 ggctgacaag agccggggaa tatctgaaaa tgacagagga aaatgtgacc cagatcgcat
     6361 taaaatgcgg gtttgaaaat ctgtcttatt ttaaccggtt gtttcgccgg aagtttggtg
     6421 tgacgcccag gcagtaccgg gaagagcagg aagctgcagc aggggtgtca ggagagtctt
     6481 cgcctttgtt gtaggagaag gggaaatgtg ctacactaag gtataagtat aaatgaaagg
     6541 caagtacagg aaagatttta cgggtacatg tacttttaag caaaagggga taaataatat
     6601 gagagaatgc ggcatgcttc ttccaatcgc cagtctgcca tctaagtatg gcattggctg
     6661 tttttcaaaa gaagcttata aatttgtaga tcagttaaag gaagcaggac agagcttctg
     6721 gcagatcctt ccattgggcc ctacgggttt tggggattcg ccttatcagg cattttctgc
     6781 ttttgcagga aatccttatt ttatcgatct ggagcagctg gtcggagaag gtcttcttac
     6841 agaagatgag tgtgaagagg ctgattttgg tgatgatgac cggtatattg cttacgataa
     6901 aatatacaaa gcgcggttta aggtactgaa aaaggcttat gaacggtgga aaaaagacca
     6961 tcatcagttc cttaagtcta agctggatcc tgaaaccata gaatactgct tttatatggc
     7021 agtaaaagat catttaaaaa gtgccagctg gaacaaatgg gataaagata tccgcctgcg
     7081 taagcctgcg gcagtagaga aatataaaaa aatgctggca gatgagattg gattttatga
     7141 attccagcag tttgaattta atgagcagtg gagccgctta aaggcttatg ccaatgaaaa
     7201 tggtatccgt attatcgggg atatccccat ctatgtagct tttgacgggg ctgacagctg
     7261 ggctcatccg gagttgttcc agtttgatga ggaatgtctg ccaaaggcag tggccggctg
     7321 cccaccggat gcattttcag caacaggcca gctttgggga aatccgttgt atgattgggg
     7381 atatcataag aaagatcatt acagctggtg gatcaagcgt atggaataca gcctgaagct
     7441 gtatgatgtg gtgcgtgtgg atcatttcag aggttttgag gcttattatt ccattcctta
     7501 tggagatcct accgctgagt ttggacattg ggaaaaggga ccgggactgg atctgtttgc
     7561 ccggttgaaa aagagtctga aaaaagaaga actgccgatc attgcggaag atctgggatt
     7621 tctgacaccg gaagtccatg agatgttaaa agaaagcggc ttcccgggaa tgaaggtact
     7681 ggaatttgcc tttgatgatt cagaaaacag cgattacctg cctcataaat accaggaaaa
     7741 ctgtattgtg tatacaggaa cccatgataa tgagacactg gtgggctggt atgagaacat
     7801 gaaccgtaga aataagtcct ttgcaaggga atacatgggt tgccgccata caccaaagaa
     7861 ggagatgcac tgggagttca tccgcctggc tatggcaagt ccggcgaagc tggcagtgat
     7921 accggttcag gattatctgg gacttggggc agaggcccgg atcaatgagc cttccacact
     7981 tggaaaaaac tggaaatgga gactgctacc gggagaaata accggggatg ttttgaaaca
     8041 gtgccggaga atggcaaaat tgtacggacg ccttccggaa acagtgtcag acataaatat
     8101 gtaatatgtt taaggaattg ttatgtaaat attcaaaatt aattcatgta tttgcattaa
     8161 ttatctgata aaatataatc atccgaagga tagaactata tccgattttc aggaggggta
     8221 gatatgagag atcagaaacg ttggatgaaa aactggatca gggcattggc tgtgggaacg
     8281 atgatcactg cagcggttcc catttctgtt atggcagcag aaaaaggctg ggtcaatgag
     8341 gacggtgcgg cccattatgt ggacagcaat ggctcttatg taaaaaatca gtggaaaact
     8401 tcccagggag tttcctatta tctggatgaa aacggagatc tggcgaagaa tgtatgggtt
     8461 gacggtactt actatgccgg agaagatggc agtatgacaa aggatgcctg gatctatgtg
     8521 gatgaaaatg atggatccat gaaatccggc tggtattatc tgaaccggaa tggaaaagtg
     8581 gagaagaatg actggaagac cataggaaaa ggcaagtatt gttttgacag cgatggacgg
     8641 atgcgtaccg gctggcatta tgagaacgga gatatttatt atctgggaaa tggttcggaa
     8701 ggttatacca gaagtggctg gtattttctg gaaagtgatg gaaagaaacg tccggcagag
     8761 ggaagtgttt caaaggatct ttccggggag tctgaaaatg gaagatggta ttatttccag
     8821 tccaacggca aagccagaaa aggtgaaaat ggtgctccaa aggaaacaac cattgatgga
     8881 aagaaatatt attttgacga aaatggtgtt atgcttaccg gctggcagtg tgtaaaggaa
     8941 aaggcagagc cgggagacgg gacaggcatc agccgttttg tatatctggg cggaaaagaa
     9001 aaaggcatgt taaagggcca gtggtttgag acaactgaaa agccatggga tacaaaggca
     9061 tggagtacag ctcttaatac ccaggcagtg agaaagaaca acacagacga gacagctctt
     9121 atgaagaaag acggatcccg atggttctat ctggaaaatg atggaactcc cctgttttta
     9181 tctgcggatg caacaggatt aaaagacggg gctgtaaaat tagacggaag atattatttc
     9241 tttgactctt acggtgtctg ccagtccgga atgatcaaat ttacttccgg cggtaagaca
     9301 gagaccgctt actttgatcc ggaagcaggc ggcgccctga gtattggacg caataccaat
     9361 gtagttgata agagagacaa ggtttacact ttttgttccg gtacttccgg ctctgaaaag
     9421 ggctgcggca ttaacggcgt gaaggatgga tttttgtatt ataatggcct tttagtcagt
     9481 gccaaagagg gcagctccta tgagcctttt aaggtttccg gtcaggttta cctggtaaat
     9541 gaagcaggaa aggtgcagac agaggaaaag aagtattctt ctgatggaaa tgctgcatac
     9601 actattgaaa atggaaaagt atttacttta gatgaaaatg gtgagaaaaa gagcgcggcc
     9661 acaggtttaa gccttcctta tgcaggttgg aagatccagt atcagagata gaagtgaatg
     9721 gaggaaacaa acatagatat aaaaatggtt acagttcaga catgacatct tcttgtttcc
     9781 atattcatga aaacaagaag cctgatggtt ccaccatatg ctgatttgaa caagaaagcg
     9841 cttatgcaag caagcttgca acacactttc tcattcaaac cagcgcatgg ctgaactgta
     9901 acacaaaatg ctgcagggac atcggatcct gcagcatttt tgcacttcac tgtatacatg
     9961 ggaatacttg aaaaaaaaat ggcggtctgc tatacttgac taacgaaaaa cttcgtaaaa
    10021 tgaggagacc ccggtcgttt ccaaccgggg caattatgaa aaatctatgt gcaatttgac
    10081 taataacctg tttttttatt acctctttct tatgaaaata gtataaaaaa taaatgtgaa
    10141 cgaaatgtga acatattgta aacagattat aaaacattgt acaggaaaaa gaaacaatat
    10201 gtgtaaatca aaagacaaaa gaatgagcgc gataagcatc tcagggaggg agaaatggaa
    10261 caggaattaa acagaaaaac catggtgatc ggacatagaa atccggatac ggactcgatc
    10321 tgttccgcca tttgttatgc aaatctgaag caggcagtta ctggggaaga atatatgcct
    10381 gcaagagccg gacatgtaaa tggagagaca cagtttgtac tggattactt tggggcagag
    10441 gaaccgcagc ttgtggaaga tgtgcgtacc caggtaaggg atattgagat ccgcaagacc
    10501 aaaggtgtgg cagacaatat ttcattaaag agagcatgga atatcatgca ggagaataat
    10561 gttgtgacca ttccttctgt aagggaagat ggtacactgg aaggcctgat cacagtagga
    10621 gacatcacca agacctacat gaatatttgt gacagcagca ttctgtcaaa ggcaaataca
    10681 cagtattcca atattattga gacactggaa gcagaactga tcatcggaag cgcagaagct
    10741 tatttcgac
//
LOCUS       DBMDUA010000092        10609 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3072_length_10609_cov_2.636277, whole genome shotgun sequence.
ACCESSION   DBMDUA010000092 DBMDUA010000000
VERSION     DBMDUA010000092.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10609)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10609)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10609
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3072_length_10609_cov_2.636277"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            58..456
                     /locus_tag="ACZPCL_08115"
     CDS             58..456
                     /locus_tag="ACZPCL_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162465.1"
                     /translation="MASATKNVLKDAGNLFSDTAHEAVSAAVDTAHIAIHSMKAKKDD
                     LFEEEDDPKKGDSFTEEDYLDDDYVDDDDLYDYERLDHSPSPAKEENISVSVNPEAAD
                     DLEEESEEKEETAKAAEPEKETATIEEDTI"
     gene            complement(563..2239)
                     /locus_tag="ACZPCL_08120"
     CDS             complement(563..2239)
                     /locus_tag="ACZPCL_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1162466.1"
                     /translation="MRENRISVFRKHTLGILVALGMVLFGSVFILFTNIGKNKATVAA
                     EETLQFIKEQSSRSEAFAEFYQGLHLEMNGIIMICKNGLVTGSNFEEYQGKTQAEAPL
                     IQNITEETEPDTLFSFRYYGSAYFGVFESYGENDLYVFYPVASVFFLRTLALAYVMIL
                     YILLCSVFLWLRQREEKEQEEREMACQEAVLLSATKEEKANAAKSDFLRKLSYDIRTP
                     INGIRELVELGNRYDHDLEKQRECRKKIWETSDVLLDLISDMVDMDKLESGAVDLELR
                     SFDMRELYEDVCQWAKELAADRGIRYHSRQLSGKNWKLYGSPSHLKQILKNVVGNAIK
                     DNRGGDCVELSCEERQSAGGTAWFEFVCTDTRSSYRRGEPGLAVAKGLIQMMGGEIHF
                     SDEETGEAFCRIRLPSLIDEEQAQEKDAKVQEAVKRPVSIRGKKILLVEDNELNMEIA
                     EFLLRKEGAQVIRAWNGKEALECFEASAPGDISVILMDLVMPVMDGFQSAKAIRSLER
                     EDASKVAIIALTASDYEEDAQKCRQAGMDEHLPKPLDAGNLIRVIQKYLR"
     gene            complement(2236..4167)
                     /locus_tag="ACZPCL_08125"
     CDS             complement(2236..4167)
                     /locus_tag="ACZPCL_08125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR1162467.1"
                     /translation="MKFNAVLTKEQAKEMFAYLEDIFTEVKVLDIEAVRRLEKDKNSI
                     SLKAFRERCQKKRLEYVESRVYQRIARYVEIEGEPYVVEMLNVLGDAADSEANAREAL
                     VAKVRGYNEELYLDALTGAYNRRYYEDRIRKVKSHAGVAMIDLDDFKLYNDTCGHNAG
                     DMVLNTVVGIVRKCIRKTDILIRYGGDEFLLILPDVSEDVFSKKLKQIRDMVYEAEVP
                     GYTQLHLSVSIGGVLTDNETVESAVGRADSLMYQAKNRKNMTVTEKSGIDSVKGREKQ
                     QVLIVDDSQMNREILAVILEEEYKILEAANGEECIDLLKQYGTGISLILLDINMPVMD
                     GFEVLALMNRNHWIEDTPVIMISSEDGAAYVRRAYEMGASDYISRPFDAQVVHQRVFN
                     TIKLYAKQRHLISLVTDQIQEKEKHNRMMVSILSQIVEFRNGESGSHVLHINTITGML
                     LERLMQKTDQYHLLWSDRFMITTASALHDIGKIGIDEKILNKPGKLTKEEFEIMKTHT
                     LIGASMLDSLEMFRNEKLVQVAYQICRWHHERYDGKGYPDGLKGEEIPIAAQVVAMAD
                     VYDALVSERVYKKAFSHEKAIEMILNGECGVFNPILLGCLVDIQDRLKEELQSGFQKK
                     KNEDLYPVLSEEIKEETEK"
     assembly_gap    4185..4194
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4379..5284)
                     /locus_tag="ACZPCL_08130"
     CDS             complement(4379..5284)
                     /locus_tag="ACZPCL_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162468.1"
                     /translation="MKINGKKLPGQWLAALVAYGLALPTAGFLGMSRALPATEPPVME
                     QTEATEETIPESSEVPESDMETETAQEIVEENSFSARYEALPQDQKQLLEDIRVAVAA
                     EDMVQAAKLLDKKQEVLAQIFYGTFEGERFLYDGSTISEEIEGEGLVFTMPEILFYGT
                     FQDGQPQGNCLALQFVNVDSPRYNFSNGVWSSGKMNGQGATGYCYYENVPQGEYAWVE
                     KQGQFVDDEMEGSISYEIRGTDDTLSHWDMNVSQGKIQLDERWTHLDDAGEYQLMSAD
                     SVSHAYLIADDQVDQVLWMNLLGWD"
     gene            complement(5286..6707)
                     /gene="pdxR"
                     /locus_tag="ACZPCL_08135"
     CDS             complement(5286..6707)
                     /gene="pdxR"
                     /locus_tag="ACZPCL_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070279 - vitamin B6 binding [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:2000142 - regulation of DNA-templated
                     transcription initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MocR-like pyridoxine biosynthesis transcription
                     factor PdxR"
                     /protein_id="HIR1162469.1"
                     /translation="MELMVPIENKDSIPLYEQIYRYIKTEIRAGRLRAGSRLPSTRTL
                     ADNLHVSRSTTQLAYEQLLSEGYIEALPCRGYFVCQIEELVEVKYHGVPEEKEEKQEE
                     NENYNYKVDFSPRGIDLDSFPFNAWRKLSKNTLVDDNKDMFAVGNAQGEYSLRQVIRD
                     YLHSARGVHCRPEQILVGAGSEYLLMLLSQLIGRSTGIALENPTYKQAFRVFESLGHP
                     IFPVSMDEWGMKVKELEKTQARIAYVMPSHQYPTGIVIPIKRRQELLKWAKKQEGRYL
                     IEDDYDSEFRYKGRPIPALQGMDDSQKVIYMGTFSRSIAPAIRVGFMVLPEPLLALYR
                     EKAGFYASTVSRVDQNILTHFIIEGYYERHLNRMRSVYRGKHDTLLAGLRELEPYFEI
                     GGEYAGIHVLLTHKKGISEKKLTETAAQAGVKVYGMSDFVIGQNKDLFPSTVILGYAS
                     LKESEITEGCKRLVNAWKELEGE"
     gene            complement(6774..8468)
                     /locus_tag="ACZPCL_08140"
     CDS             complement(6774..8468)
                     /locus_tag="ACZPCL_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIR1162470.1"
                     /translation="MAEKDIVVETEEKELVSKNFIEQEIDKDLAEGVYDHVQTRFPPE
                     PNGYLHIGHAKSILLNYGLAQKYNGKFNLRFDDTNPTKEKTEFVESIMADVKWLGADF
                     EDRLFFASNYFEKMYECAVFLIKKGKAFVCDLSAEQIREYRGDFNTPGKESPYRNRSI
                     EENLRLFEEMKEGKYQDGEKVLRAKIDMASPNINMRDPVIYRVAHMHHHNTGDKWCIY
                     PMYDFAHPIEDAVEHITHSICTLEFEDHRPLYDWVVRECEFENPPRQIEFAKLYLTNV
                     VTGKRYIKKLVEDGIVDGWDDPRLVSIAALRRRGYTPESIKRFVELVGVSKANSSVDY
                     AMLEYCIREDLKLKKSRMMAVLDPVKLVIDNYPEGQVEELDVPNNLENPELGSRKVPF
                     SREVYIEREDFMEEPPKKYFRMFPGNEVRLMGAYFVKCTGCEKDENGNVTVVHGTYDP
                     ETKSGSGFEGRKVKGTIHWVAVPTAKKIECRLYENIVDEEKGKLNADGTLNLNPNSLT
                     VLKECYGEPALAQAEAYDSFQFVRNGYFCADCKDSTKENPVFNRIVSLKSSFKLPK"
     gene            complement(8513..8884)
                     /locus_tag="ACZPCL_08145"
     CDS             complement(8513..8884)
                     /locus_tag="ACZPCL_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1162471.1"
                     /translation="MVEDNELNREIAQFILEHTGITVVNAENGKEAVEIFKASEQGEF
                     DFILMDVMMPVMDGLEATRIIRKMERPDAVQIPIFAMTANAFSDDRKRCKEAGMTEHL
                     AKPLNSEKMIAKLRLYYNRKV"
     gene            9098..9367
                     /locus_tag="ACZPCL_08150"
     CDS             9098..9367
                     /locus_tag="ACZPCL_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162472.1"
                     /translation="MKSETMPKKKKSYLSRLGMTLLTILLIVLFIFIMSLVSKIQGTA
                     RVVNYAGLVRGKTQRIIKLEISGSPQDEMIADIDAFIDGLENGSS"
     gene            9410..>10609
                     /locus_tag="ACZPCL_08155"
     CDS             9410..>10609
                     /locus_tag="ACZPCL_08155"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1162473.1"
                     /translation="MKELDAYFSQLKEEVAQVRMRDYRKTDIIAKSERFFNICDEATG
                     LAEVYSQKKATQLNQLEKIVFADIAGLLILIGAELVKTLRYAALNRLLQSKVYLDEAT
                     GLPNKNKCEELLNAPKPVPADAFAAVCVFDLNNLRIINNTMGHEMGDAYIRSFADQLY
                     QAIPDDQFAGRDGGDEFIAIIKDTESSAFHAFLFKLKQLCADYSRLHPDMPISYAAGY
                     ATSEDFEGSTLRELFRYADKNMYIDKNRAKLEEAAKEKKLHLELLSSIRSQGFHFSDC
                     IYCDALTDQYKVLRASSDFFLADDGSYSGAVEQILSQVSATENIKALRKQLQLETLNE
                     KLMENNSPIRLFCQYTANGISHKGRLTLLYLDANASGQLHHFILGFEIFQNMENTKTD
                     EKTQLNQY"
BASE COUNT         2714 a         2678 c         1999 g         3208 t
ORIGIN      
        1 tcaccttctg ccggagttgg ctgataatta ttatatccat cataggcagc aaaagatatg
       61 gcatctgcta ctaaaaatgt attaaaagat gcaggaaatc tgttctccga tacagcccac
      121 gaagcagttt ccgctgctgt agataccgca catatcgcta tccactccat gaaagcaaaa
      181 aaagacgatc tctttgaaga agaggatgat cctaaaaagg gagactcttt tacagaagaa
      241 gattatctgg atgatgacta tgtagacgat gacgatcttt acgactacga acgtctggat
      301 cactctcctt caccagcaaa agaagaaaac atctctgttt ctgttaatcc ggaagccgct
      361 gatgatctgg aagaagaaag cgaagaaaaa gaagagacag ccaaggcagc tgaacctgaa
      421 aaagaaacag ctaccatcga ggaagatacc atctaatctg atttaaccag actttaaaag
      481 agctgcacag ccccaaagcc gtgcagctct tttttcttaa tttttctgat accggattgc
      541 tatgttcttt gcactcaccc aatcatctta agtatttctg gatcacccgg atcaaatttc
      601 ctgcgtctaa tggttttgga agatgttcat ccattcctgc ctgacggcat ttttgtgcat
      661 cttcttcata atcgctggct gtaagggcga tgatcgctac tttagaggca tcttcccgtt
      721 ccaggctccg gatcgctttt gctgactgaa atccatccat caccggcatt acaagatcca
      781 taaggatcac ggaaatatct cccggagcag aagcctcaaa acattctaac gcttcttttc
      841 cattccaggc tcttatgacc tgggctcctt cttttcgcaa aaggaactcc gcgatctcca
      901 tattcagctc attatcctcc actaaaagga tcttctttcc ccggatggat acaggacgtt
      961 tcacagcttc ctggaccttt gcgtctttct cctgcgcctg ttcttcatca ataagggacg
     1021 gcagacggat cctgcagaat gcttcgcctg tttcttcatc tgaaaaatgg atctccccgc
     1081 ccatcatctg gatcaggcct ttggcaactg ccaatcccgg ttcccctctt ctgtaagaac
     1141 ttcgggtatc tgtacagaca aattcaaacc aggctgtccc tcccgcggac tgacgttctt
     1201 cacaggaaag ttctacacag tcaccaccac ggttatcttt aatagcattt cccacaacat
     1261 ttttcaggat ctgctttaag tgagacggac tgccatataa tttccagttt ttcccagata
     1321 actgtctgct gtggtaacgg atccctctgt cagcagcaag ctcttttgcc cactggcaga
     1381 catcctcata taactccctc atatcaaaag atctcagttc caaatccaca gcgccagact
     1441 ccaacttgtc catatccacc atgtctgaga tcagatctaa cagcacatct gaggtctccc
     1501 atattttttt cctgcattct ctctgctttt caaggtcatg gtcataacga ttgcccaatt
     1561 ctaccagttc ccggatgcca ttgataggag tgcggatgtc ataactgagc ttgcgaagaa
     1621 agtctgattt agctgcattt gccttttctt cctttgtggc agataacaaa actgcctcct
     1681 gacaggccat ttccctttct tcctgctcct tttcttctct ctgacgcagc cataagaaaa
     1741 cactgcataa aaggatataa aggatcatga cataagctaa tgccagggtc ctcaggaaaa
     1801 aaacagaagc cacaggataa aatacgtaca gatcattctc tccataagac tcaaagaccc
     1861 caaaataagc agatccgtag tagcgaaagg aaaataaagt atccggctct gtctcttctg
     1921 ttatattctg gatcaaaggt gcctctgcct gcgtttttcc ctgatattcc tcaaaatttg
     1981 atcctgtcac aaggccattt ttgcagatca ttatgatccc gttcatttcc agatgaaggc
     2041 cctgataaaa ctctgcaaat gcctctgacc ggctgctctg ttcctttata aactgtaacg
     2101 tttcttctgc tgccacagtc gctttgtttt ttccaatatt tgtaaataaa atgaatacag
     2161 aaccaaacag aaccattccc aaagcaacca gtatacctaa ggtatgtttt ctaaaaacag
     2221 atattctgtt ttctctcact tttctgtctc ctcttttatc tcctctgata ataccggata
     2281 aagatcctca tttttcttct tttgaaatcc ggactgcagt tcttctttta accgatcctg
     2341 gatatccacc agacatccta acaggatggg attaaagact ccacattccc cgtttaagat
     2401 catttcaatg gctttttcgt gggaaaatgc cttcttgtat acacgctcac ttacaagggc
     2461 gtcatataca tctgccattg caaccacctg ggctgcgatc gggatctctt ccccctttaa
     2521 tccgtccgga tacccttttc catcataccg ctcatggtgc caccggcaga tctggtatgc
     2581 aacctgtacc agtttttcat tccggaacat ttccagactg tccaacatag aagcaccgat
     2641 cagtgtatgg gtcttcatga tctcaaattc ttcttttgtc agttttcccg gcttgtttaa
     2701 tattttttca tcaattccta ttttcccgat atcatgaagt gcggaagctg ttgtgatcat
     2761 gaaacggtct gaccacagca gatgatactg gtctgttttc tgcatcagac gttccagcag
     2821 catgcccgtg atcgtattga tatgtaatac atggctgccg ctttctccat ttctgaactc
     2881 aacgatctgg cttaagatgc tgaccatcat ccggttgtgt ttctcttttt cctggatctg
     2941 gtctgtaacc agactgatca gatgtctctg ctttgcgtaa agtttaatgg tattaaacac
     3001 tctctgatgt actacctggg catcaaaagg acggctgata taatcagatg cccccatttc
     3061 ataagccctt ctcacataag ccgcgccatc ttcgctggag atcatgatca ccggcgtgtc
     3121 ttcgatccag tggtttcggt tcatcagcgc caatacttca aaaccatcca tcaccggcat
     3181 attgatatcc aaaaggatca gtgagatacc ggttccatac tgctttaaca gatcaataca
     3241 ttcttccccg tttgcagctt ctaagatctt gtattcctct tccaggatca cagcaaggat
     3301 ctcacggttc atctgggaat catctacgat caggacctgc tgcttttccc tgcctttcac
     3361 actgtcaata ccggactttt ctgttaccgt catatttttc cggttctttg cctgatacat
     3421 caggctgtcc gctcttccta ctgcgctttc cactgtttca ttatcagtca aaacgcctcc
     3481 aatgcttaca gacaggtgaa gctgtgtata tccgggaact tctgcctcat agaccatatc
     3541 ccggatctgt tttaacttct ttgaaaaaac atcttccgac acatccggaa ggatcagcag
     3601 aaattcatcg cccccatacc ggatcaggat atctgttttt cggatacatt tgcgcacgat
     3661 ccccacaact gtatttaaca ccatatcccc tgcattatgt ccgcaggtat cattgtacag
     3721 tttaaaatca tccagatcga tcattgcaac tcctgcatga cttttcactt tgcggatcct
     3781 gtcctcataa tatctgcggt tgtaagctcc tgtcagcgca tccagataca gttcttcatt
     3841 atatccccgg acttttgcca ccagtgcttc ccttgcattt gcctctgagt ctgctgcatc
     3901 ccccagaaca ttcaacatct ctacaacgta aggttcccct tcgatctcca cataacgcgc
     3961 gatcctctga tataccctgg actcaacgta ttccagtctt tttttctggc accgttccct
     4021 gaatgccttt aatgaaatag aatttttatc tttttccaga cgcctgactg cttctatatc
     4081 caggaccttt acttctgtaa atatgtcttc taaataagca aacatttcct ttgcctgttc
     4141 ttttgtcaaa acggcattga atttcatgtc tttctccttt acagnnnnnn nnnnagtgaa
     4201 atgtttcctt tctgttgttg aagtttcatc tcagccagaa ggcgctccaa cgaagttatt
     4261 tctttctcct ttacagtctg gtttccgtca ttttatccat tcatgtatcg tatcctattt
     4321 aaccggattt ttcttttaga tattatatct attttcccct ctgttgtcca tatttcattt
     4381 aatcccagcc cagaagattc atccacaata cctgatctac ctgatcatct gcgatcagat
     4441 aggcatggct gacagagtcc gcagacataa gctggtattc gcctgcatca tccagatggg
     4501 tccaacgttc gtctaactgg atctttccct gggaaacatt catatcccag tgggacagcg
     4561 tatcatctgt tccacggatc tcataggaaa tgctgccctc catttcatca tccacaaact
     4621 gtccctgctt ttccacccag gcatattccc cctggggaac attttcataa taacagtagc
     4681 cagttgctcc ctgtccgttc atttttccac tgctccatac accgtttgag aaattatatc
     4741 tggggctgtc cacatttaca aactgtaatg ccagacagtt tccctgaggc tggccatcct
     4801 gaaaagttcc gtaaaacagg atctccggca tggtaaatac cagtccctct ccctctattt
     4861 cctctgaaat ggtactgcca tcataaagga aacgttctcc ttcaaaggtt ccgtaaaaga
     4921 tctgggcaag aacctcctgt tttttatcca gaagcttggc agcctggacc atatcttccg
     4981 ctgccactgc cacacggata tcttccaaaa gctgtttctg atcttgcgga agtgcctcat
     5041 atctggcact gaacgaattt tcttctacga tttcctgcgc tgtttccgtt tccatatcac
     5101 tttccggcac ttcgctcgat tctggtatgg tttcttctgt tgcttcggtc tgttccataa
     5161 ccggcggctc tgttgccggt aaagccctgc tcattcccaa aaatccagct gttggaagag
     5221 ccagaccata agccaccaat gctgccagcc actgtcccgg aagctttttt ccatttattt
     5281 tcaatttatt caccttctaa ttctttccag gcatttacaa ggcgtttgca gccctctgtg
     5341 atctcacttt cctttaaact tgcatatcca agtataacgg tagacggaaa aagatcttta
     5401 ttttgtccta ttacaaaatc tgacattcca tagactttta cacctgcctg tgctgcagtc
     5461 tctgtcagtt tcttttctga aatccctttc ttatgggtaa ggagaacatg gatccctgca
     5521 tactcaccgc cgatctcaaa atagggttcc agctctctta atcctgcaag aagcgtatca
     5581 tgttttcccc tgtaaacaga tcgcatcctg tttaaatggc gctcataata tccttctata
     5641 ataaaatgag tcaggatatt ctggtccact cttgatacag ttgatgcata aaaacctgct
     5701 ttttcccggt ataaagccag caacggctcc ggaagtacca taaagcccac acggatcgcc
     5761 ggagcaatgg aacgggaaaa ggttcccata tagattacct tctggctgtc atccattccc
     5821 tgaagtgccg gtatcggacg gcctttataa cggaattcac tgtcgtaatc atcttcgatc
     5881 agataacggc cttcctgttt tttggcccat ttcagcagct cctgccgcct tttaataggg
     5941 ataacgattc ctgtggggta ctggtgggac ggcataacat aggcgatccg tgcctgtgtt
     6001 ttctctaact ctttcacctt cattccccat tcatccatag atacaggaaa aataggatga
     6061 cccaggcttt caaatacacg aaaggcctgc ttgtaagtgg gattttccag ggcgataccc
     6121 gtactgcggc cgatcagctg ggaaaggagc attaaaagat attcacttcc cgcacctacc
     6181 aggatctgtt ccggacggca gtggactcca cgggcagagt gaagataatc ccggatcacc
     6241 tgacgcaggc tgtattctcc ctgggcattt cccactgcaa acatatcttt gttgtcatct
     6301 accagcgtat ttttgcttag ttttctccag gcattaaaag ggaaactgtc taggtcgata
     6361 cctcttgggg aaaagtctac tttgtaattg taattttcat tttcttcctg cttttcctct
     6421 ttttcttccg gcactccgtg atattttact tctaccagtt cttcgatctg acagacaaaa
     6481 tatcccctgc agggaagagc ctctatgtat ccctctgata gcagctgttc ataagccaac
     6541 tgggtcgtac tcctgcttac atgaagattg tccgcaagtg tcctggtaga aggaagtctg
     6601 ctgccagccc gcagtctgcc ggcccgtatt tctgttttta tgtagcggta gatctgttca
     6661 taaaggggga tgctgtcttt attttcaata ggcaccatta attccatagg gcttctccta
     6721 acatgaaaaa ggctctctgt ttccagagag cctcattgct ttgcttcact tcattatttc
     6781 ggcagtttga aagaactctt cagtgaaacg atccggttaa atacaggatt ttccttggtg
     6841 ctgtccttgc agtctgcaca gaaataacca tttcttacaa actggaagct gtcataagcc
     6901 tccgcctgtg caagagcagg ttcaccgtag cactccttta atacagtaag ggaatttgga
     6961 tttaagttca gggttccgtc tgcattcagc ttaccctttt cctcatctac gatattttca
     7021 tagagacggc actcgatctt ctttgcagtt ggtacagcta cccagtggat ggtacctttt
     7081 acctttctgc cttcaaagcc ggaaccgctc tttgtctcag gatcataagt tccgtgtact
     7141 acagttacat tgccattttc atctttttcg cagccagtgc attttacgaa ataagcgccc
     7201 ataagacgta cttcgtttcc tgggaacata cggaaatatt tcttaggcgg ctcttccata
     7261 aagtcttctc tttcgatata aacttcacgg gagaatggaa cctttctgct tcccagttcc
     7321 ggattttcca ggttgtttgg aacatccagt tcttctacct gtccttccgg atagttgtcg
     7381 atcaccagtt ttacaggatc taacactgcc atcatacgtg atttcttcag ttttaaatct
     7441 tcacggatgc agtactcaag cattgcatag tcaacagagc tgtttgcctt tgacacgcca
     7501 actaactcta caaaacgctt gatggactcc ggtgtataac ctcttcttcg cagtgcagcg
     7561 atggatacca gacgaggatc atcccaaccg tctacaattc cgtcttctac cagcttcttg
     7621 atataacgct tacctgtaac cacgttggtc aggtacagct ttgcaaactc gatctgacga
     7681 ggtggatttt caaattcgca ctctcttact acccagtcat acagaggacg gtgatcctca
     7741 aattccaggg tacagatgga atgggtaata tgctctaccg catcctcgat cggatgagcg
     7801 aagtcataca tcggatagat gcaccattta tcacctgtat tgtgatgatg catatgagct
     7861 acacggtaga tgacagggtc tctcatgttg atgttagggg aagccatatc gatttttgcg
     7921 cgaagaacct tctcgccgtc ctgatacttg ccttccttca tctcctcaaa gagacgcagg
     7981 ttctcttcga tggaacggtt tctgtatggg ctttccttac ctggtgtatt gaaatcgcct
     8041 ctgtactctc tgatctgttc agcgcttaaa tcgcagacaa aggcttttcc cttcttaata
     8101 aggaaaacag cacactcata cattttttca aaatagttgg aagcaaagaa cagtctgtcc
     8161 tcaaaatctg ctcctaacca ctttacgtct gccataatgg actcaacaaa ctctgttttt
     8221 tccttggttg ggtttgtatc atcaaaacgc aggttaaact tgccattata tttctgtgcc
     8281 agtccatagt ttaaaagaat agacttggca tgtccgatat gcagatagcc atttggttcc
     8341 ggtggaaaac gggtctgtac atggtcataa acgccttctg ccaggtcctt atcgatttcc
     8401 tgctctataa agttcttaga aaccagctct ttttcttcgg tttctactac aatgtctttc
     8461 tctgccataa ctgctcctcc gtgatctgtc gtcatagtta tttaaaataa tatcacactt
     8521 tccggttata gtaaagccgc agctttgcga tcattttctc tgaatttagt ggttttgcta
     8581 gatgttccgt cattccggcc tccttgcagc gctttctgtc atcggaaaaa gcattggctg
     8641 tcattgcaaa aatagggatc tgcactgcat ccgggcgttc cattttccgg atgatccttg
     8701 ttgcctccag tccgtccatg acgggcatca tcacatccat taaaataaaa tcaaactctc
     8761 cctgttcgga agccttaaat atttccactg cttcttttcc gttctctgca ttgacaactg
     8821 tgatccctgt atgctctaag ataaactggg caatttcccg gttcagctca ttatcctcta
     8881 ccaactgaca tcctctacag gatccggatc ttccaatacc aggatcagtc cccatttatc
     8941 aactgccaag tcatactttt ttattattac aagtgtaaca tgaggggcat aaaatatcaa
     9001 gtttgtacct gctttgtaac cacatatttt cattcttgcc aagaaataga aaatagaata
     9061 aaatggttac aaaacagtgg aatggagtta tatgaaaatg aaatcagaaa ccatgcctaa
     9121 aaagaaaaaa tcctacctgt cacggcttgg catgacactt ttaactatac tgctgatcgt
     9181 tcttttcatt tttatcatgt ccctggtaag taagatccag ggtactgcca gagtggtcaa
     9241 ttatgccggt ctggtccgtg gaaagaccca gcgtattatt aaattggaga tctccggaag
     9301 cccccaggat gaaatgatcg ctgatattga tgcctttatt gacggtctgg aaaacggaag
     9361 cagttagtta aacctggtgc gcttagatga tcctgacttt caggccaaaa tgaaagaact
     9421 ggatgcctat ttttcccagt taaaagaaga ggtcgcccaa gtccgtatga gggattaccg
     9481 taaaacagat attatcgcca aaagtgagcg ttttttcaat atctgcgacg aagctaccgg
     9541 ccttgcagaa gtttactccc agaaaaaggc cacacagtta aaccagttgg aaaagatcgt
     9601 atttgcggat attgcaggcc ttcttatcct gatcggtgca gagctggtaa aaacccttcg
     9661 gtatgctgct ttaaaccgcc tgctccaaag caaggtatat ttagacgaag ccaccggcct
     9721 tcccaacaaa aataaatgtg aggaactttt aaatgcaccg aaacctgtac ctgcagatgc
     9781 ttttgcagca gtatgcgtct tcgacttaaa taaccttcgt atcatcaaca ataccatggg
     9841 acatgaaatg ggagacgcct atatccgttc ctttgctgac cagctgtatc aggcgatccc
     9901 tgatgaccag tttgcaggaa gagatggcgg tgatgaattt attgccatta taaaagatac
     9961 agaaagcagt gctttccatg cttttctttt taagttgaaa cagctgtgtg cagactattc
    10021 ccgtctccat ccagatatgc ctatcagcta tgcagccgga tatgccactt cggaagattt
    10081 tgagggaagt acccttcggg aactgttccg gtacgcagat aaaaatatgt acattgacaa
    10141 aaaccgggca aaactggagg aagcggcaaa agaaaagaaa ctgcatcttg agctgctttc
    10201 ttccatccgt tctcagggtt tccatttttc cgactgtatc tactgtgacg ccctgacgga
    10261 tcagtataag gtactgcgtg ccagctccga tttcttctta gcagatgacg gaagctactc
    10321 cggtgcagtg gaacagatcc tttcccaggt ttctgcaacc gaaaatatca aagcactgcg
    10381 aaagcagctg cagttggaaa ctttaaatga aaaactgatg gagaacaatt cccccatccg
    10441 cctgttctgt cagtatactg ccaacggtat ttcccacaaa ggccgtctga ctttgcttta
    10501 tttagatgcc aatgcaagcg ggcagcttca tcattttatc cttggctttg aaattttcca
    10561 aaatatggaa aatactaaaa cagatgagaa aacacagtta aatcagtat
//
LOCUS       DBMDUA010000093        10536 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3089_length_10536_cov_2.848682, whole genome shotgun sequence.
ACCESSION   DBMDUA010000093 DBMDUA010000000
VERSION     DBMDUA010000093.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10536)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10536)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10536
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3089_length_10536_cov_2.848682"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(48..959)
                     /locus_tag="ACZPCL_08160"
     CDS             complement(48..959)
                     /locus_tag="ACZPCL_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HIR1162474.1"
                     /translation="MKSDGSVFTLENEELLIMVERHGSQLSRIYDKKADREILWSADP
                     AVWNRHSPILFPFIAKSVNGKYRYEGKEYNISSHGFARDMDFEPVLCDMDECTYVLKD
                     TPETMEKYPFHFELEITHRLKGRTIETSWKVTNLNGKEMLFMIGGHPAFQVPEGKNIY
                     DYTLVFNKEGVKAGEHQDSLHYQAPNAEGYEDRSLQGDLKLTDGKVPVTPGFFDTALT
                     YMFDHEQISSVGLLTDGKPYVTVLCDGFPFVGVWTIEKTHPFVCLEPWYGVCDSKNFT
                     GELKERQGIQSLKAWETWEKGYSIRIE"
     gene            complement(991..1488)
                     /locus_tag="ACZPCL_08165"
     CDS             complement(991..1488)
                     /locus_tag="ACZPCL_08165"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR1162475.1"
                     /translation="MKFLVTADNNGAIGRGAQPLVTIPAMQQSFLKETQGKVVVMGRK
                     TLEGLPGGQPLGNRVNVVLSENPAYKIKGAVVCHNIDEALEYLKQFSSEDIYVIGGES
                     IFRQFLPYCNTVYLTHIDYEYDADVKFPVNLWKSQEWEMVEEGEEQTYFSLCYTPEIF
                     VRKQV"
     gene            complement(1485..1997)
                     /locus_tag="ACZPCL_08170"
     CDS             complement(1485..1997)
                     /locus_tag="ACZPCL_08170"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR1162476.1"
                     /translation="MNLIAAADENWGIGKNGGLLAHISGDMKYFRETTKGKIVVMGRK
                     TLESFPGGKPLKNRVNIVLTGNKDFVPEDVVICHSVEETLEKLKEYPKEDVFIIGGGM
                     IYKAFLPWCEKAYITHVYHTFEADTYLPDLEKQAGWKLTSVSDRYTNEPEGEQPAMDY
                     EFRIYERQNG"
     gene            2240..3070
                     /gene="thyA"
                     /locus_tag="ACZPCL_08175"
     CDS             2240..3070
                     /gene="thyA"
                     /locus_tag="ACZPCL_08175"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR1162477.1"
                     /translation="MSYADQLFIQNCKDILTNGVWDTDHEVRPVWEDGTPAHTIKKFG
                     IVNRYDLRKEFPVITLRRTYFKSAVDELLWIWQKKSNNVHDLKSHIWDSWADETGSIG
                     KAYGYQLGVKHHYKEGDFDQVDRILYDLKHNPLSRRIMSNIYNHHDLSEMHLYPCAYS
                     MTFNVSDDTLNGILNQRSQDMLTANSWNVCQYAVLMHMFAQASGLKVGELVHVIADAH
                     IYDRHIPMVEELLKKEPLPGPTLWVDPEVKDFYQFTTDSFKLENYQYHPFDHKVPVAI
                     "
     gene            complement(3194..6991)
                     /gene="addA"
                     /locus_tag="ACZPCL_08180"
     CDS             complement(3194..6991)
                     /gene="addA"
                     /locus_tag="ACZPCL_08180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HIR1162478.1"
                     /translation="MKWTEKQQQVIDSRNRNLLVSAAAGSGKTAVLVERIISMISEGT
                     HPLNIDQLLVMTFTNAAASEMRERIGAAVEKKLKEDPGNEHLWLQSALIPQAKIMTID
                     SFCLDLIRNHYNALDIDPAFRIGDEGELTLLKGDVMEQVLEECYETADDGFLQFSEQF
                     GSGKSDKAMEDVILQAWTFSQSHPWPDQWLDQCRQQMEKEAEGDLDSSEWMRFLLRDI
                     SLQMGELAAQAEEALEVAREENGPFVYEPMLMNDVSRLKKIREAAASGSYEAFYHAVS
                     ELTFDRLAAARSKEIDPEKKAYVSDLRIRVKKAAEKCRDTYGSQSPEAAIEGIRNTRI
                     VIQVLLDMVKRFDAAYTEAKRERNVLDFNDLEHLTLQVLTVEEEDGTRVPSQAAGELS
                     GQYEEILVDEYQDSNLVQETLIQSISRERLGQPNVFMVGDVKQSIYRFRLARPELFME
                     KYDTYSREESSHQMIELQQNFRSRASVLTCINDIFYQIMTKNLGGIRYTEETALYPGA
                     AFEETEKKAGIPVQFLVADTGTEAFKQLDEEAADYTARELEAKMIAEQIKSFTDEKNG
                     LYVWDKDGQSYRKACYKDMVILLRSMSGWSEVFVNVLMNEGIPATAQTRTGYFATAEV
                     ETVLGLLSVIDNPMQDIPMAAVLRSPMVGMTDDEMAWMIAAYKRYLQKKQDRGVYGAW
                     KLWEEIYPQAVSDENPDEDEKKDPETEQIRIGQVEIPKATAKSIWKKLEYFAAFTEEL
                     RQDSRYLPVHELLYQIYRKTGYYDYVSSMPAGETRKANLDMLAEKAAAYASTSYRGLF
                     HFVRYIEKLKKYDTDFGEASLGDESNSVRIMSIHKSKGLEFPVVFLAGMGKKFNKQDA
                     YGQLLLDADLGAAADEMDLNLRTKAPTLKKMAIRRRMELESMGEELRVLYVAMTRAKE
                     VLVMTGTDRSLENKLEKWDQLSLTDGQIPYTVLSGANSFLDWVLMAKAAVPAGHMEVK
                     QVQLTHLIGRELQRQLEKKNVKEELLKLDTKQVYDEQMKEQLDQALSYEYPYLEDTGL
                     YTQMSVSELKKQSQIGREEENIGTERKDLSEVRLDHEHEMEPESSEAEDGKKQTETGK
                     KGAFRGTAYHRALECLDLLAVTDQRKLEGDLLDLFQRGFLTEEAYESIFPWDIWKFLE
                     SPLGKRMKQAEKEGKLYRERQFMVGIPASEMGKGLQSEELVLIQGVIDAYIEEADGFI
                     LIDYKTDYVPKEDTQKGEELLKERYQVQLDYYGRALTQLTGKKVKEKWIYSFGLRKAI
                     QLG"
     gene            complement(6995..10471)
                     /gene="addB"
                     /locus_tag="ACZPCL_08185"
     CDS             complement(6995..10471)
                     /gene="addB"
                     /locus_tag="ACZPCL_08185"
                     /EC_number="3.1.-.-"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HIR1162479.1"
                     /translation="MSLQFIAGGSGSGKTRYLYEKVIKESEEHPDIQYLFIVPEQYTM
                     QTQKELVRLHPRHGLLNIDVLSFKRLAYRVFEDLGVQLPQVLDDMGKSMVIRKVAGKL
                     KKELKLYGGHLEQPGFINQLKSQISELCQYGVSVEDLAMVEEETDRVLLKDKLGDLKT
                     VYGGFKDYIESHYITAEEILDILCRKLPQWEPLKNSVILLDGYTGFTPVQYRLAELFM
                     IHSREVICCVTADPREMLYKECTMQHLFYMGRHTVCRLRKMAEEHHIPVLKEVWCDNR
                     PAWRFKESPQLDFLEQNLYRYNGKIWKNDPQDIQIFRGKNPAEETEYVCSCINEKVQK
                     EGMRYRDLAVVTGDLASYGKEIAHRFDEAGIPYFLDDKKSILENPFIELIRAALDGVK
                     DASYESIFRYLKTGFVYDETYSREEAQVLTDRMENYARALGIKGWKNWDMIWEKPCRG
                     GERLSLEELNQYRQWMLEPLKVLRQAFKEENATISSVTTVLRQVLESMKLEEKLESFS
                     NYFLERKEPGDENRAREYSQVYERVMELLERLEGLLGDEKADMKNYIQILDAGFEEIK
                     IGVLPATADQVMIGDITRSRLESVKVLFFTGVNEGIVPQRKAGGSLLNDGDREVFKSL
                     HMELAPTAREEGCIQKFYLYLMLSKPSRQLVITYAAMNGQGKSCHPSSLIGEIRRLFP
                     EMVPREKREEEWPVWTAEDGLKKLMAGLREAGDKNLEQLKEAQGTFLELFRRFYGQEE
                     YRKLVEKLTDAAFFVYEDKGIGRAAARALYGQKLQGSVTRLEQFAACAYAHFLKYGLE
                     LMERQEYQLEAVDMGNLFHQSLDQCFEVIYENGMDWSNITEEERKELVKKCVDQVTSQ
                     YGNTIMSSSARNTYLAKRVERITDRTIWALAEQVKKGDFVPSGFEVSFSAIDNLKAMK
                     IRLSEDEELLLKGRIDRLDLCEDEKHVYVKIIDYKSGNTSFDLAALYYGLQLQLVVYM
                     DAALEMEERKNPEKTAVPAGIFYYNIKDPMIKKEGEMTPEEIEKQILKQLRMNGLVNS
                     DLEVIHHLDREIQKESDVIPVAVKDGYVQEAKSSVAGEARFQALRRFVGKRLKQSGQA
                     ILKGENGLAPYKDGDRTACDYCPYHAVCGFDTKTAGYGFRRLHSMKPEEVWQEIEEKG
                     GMKEDKNGEGDE"
BASE COUNT         2538 a         2774 c         1963 g         3261 t
ORIGIN      
        1 gttttatgtc cagtggacat tattttttct attatagacc aggcagatca ctctatcctg
       61 atgctgtaac ctttttccca ggtctcccag gcttttaagc tctggatgcc ctgtctttct
      121 tttaactctc cggtaaaatt ctttgagtca catactccat accatggttc aagacataca
      181 aaggggtgtg tcttttctat ggtccagact cctacaaaag gaaatccgtc gcataatact
      241 gtaacatatg gctttccatc tgtcaaaagt cctacactgc tgatctgctc atgatcgaac
      301 atataagtca gtgctgtatc gaaaaatcct ggtgttacag gaacttttcc atctgtaagt
      361 ttcagatcac cctgaagaga acggtcctca tatccctctg cattaggagc ctgataatga
      421 agactgtcct gatgttcccc tgcttttacc ccttctttgt taaatacaag ggtataatcg
      481 taaatgttct ttccttccgg aacctgaaat gccggatgac caccgatcat aaacagcatt
      541 tcttttccat ttaagttggt cactttccag gacgtttcaa tggtacgtcc ttttaaacgg
      601 tgtgtgatct ccagttcaaa atggaacgga tatttttcca ttgtctccgg cgtgtctttt
      661 aatacgtatg tacactcatc catatcgcaa aggaccggct caaagtccat atctcttgca
      721 aatccatgag aagaaatatt gtattccttt ccctcatagc ggtattttcc atttacagat
      781 ttggcaataa atggaaaaag gataggtgaa tgacggttcc atactgccgg gtctgcgctc
      841 cacaggatct ccctgtctgc ttttttatca tagatcctgg aaagctgaga accatgacgc
      901 tcaaccatga tcagaagttc ttcgttttcc agtgtaaaca cgctgccgtc acttttcatg
      961 tttttctccc cccaatatca aatttttaca tcagacctgc tttcttacaa aaatttccgg
     1021 tgtataacac agactgaaat aagtctgttc ttctccttct tccaccatct cccattcctg
     1081 ggatttccat aaatttacag gaaactttac atctgcatca tattcataat caatatgagt
     1141 cagataaacg gtattgcaat aaggcaggaa ctgtcggaaa atgctttctc cgccgatcac
     1201 atatatatcc tcagaagaaa actgttttaa atattccagt gcttcatcaa tattgtgaca
     1261 tacaacagcg ccttttatct tataggccgg attttctgaa agtaccacat tgacgcggtt
     1321 tccaagaggc tgtccaccgg gaagtccttc cagagtcttt cgtcccatga ccactacttt
     1381 gccctgagtc tcttttaaga aactctgctg catagccggg atcgtcacta agggctgtgc
     1441 tcctctgcca atagccccat tgttatcagc tgttacaaga aatttcatcc attctgcctt
     1501 tcgtagatcc tgaattcata gtccattgca ggctgttcgc cttctggttc attggtatat
     1561 ctgtcactga cagatgttaa cttccagcct gcctgctttt ctaaatccgg cagataggta
     1621 tctgcctcaa atgtatggta aacgtgggta atatacgctt tttcacacca tggcaggaag
     1681 gctttgtaga tcatgccacc gccaatgatg aaaacatctt ctttgggata ttcctttaat
     1741 ttttcaagtg tctcttctac gctgtggcag atgaccacat cttccggcac aaaatcctta
     1801 tttccggtaa ggacaatgtt cacacggttc tttaatggct ttccccctgg aaaactttcc
     1861 agagtctttc gtcccatgac tactattttt ccttttgtag tctcacggaa atatttcatg
     1921 tcaccggaaa tatgggctaa aagtccgcca ttttttccaa ttccccagtt ttcatctgct
     1981 gctgcgatca aattcatgtc caaacccctt gtttatattt taactaccag aagaattata
     2041 acacgtagtt tttaaaattg gaagatacca atacctttta gatacctttt gctgaaattt
     2101 gttacagttt tgctgtactg catgtctgag atttgctata ctaggtaatg gaccgtctgc
     2161 gtaagcagac aacagagcgc gcccgcgcgt gtccataagt ctatattcta tgccatcgca
     2221 aaaggagaca caaaaccaca tgagttacgc agatcagctt tttatacaaa attgcaaaga
     2281 cattcttaca aacggagtct gggatactga ccatgaagtg cgccctgtat gggaagacgg
     2341 cactccggct cacaccatta aaaaattcgg cattgtaaac cgatacgact taagaaaaga
     2401 gtttccggtg atcacactgc gccgcactta ttttaaatcc gcagtggatg agctgctgtg
     2461 gatctggcag aaaaagtcca acaatgtaca tgacttaaag agccatatct gggactcctg
     2521 ggctgatgag accggcagta tcggaaaagc ctacggatac cagctgggcg tgaagcatca
     2581 ttataaagaa ggggatttcg accaggtaga ccgtatcctt tatgatttaa agcacaatcc
     2641 tttaagccgc cgcattatgt ccaatatcta caatcaccat gatttaagcg aaatgcacct
     2701 gtatccatgt gcctacagca tgacctttaa tgtatccgac gataccttaa acggtatctt
     2761 aaaccagcgt tcccaggata tgcttactgc aaacagctgg aatgtatgcc agtacgcagt
     2821 cctgatgcac atgttcgctc aggcaagcgg cttaaaagta ggagaactgg tccatgtgat
     2881 cgcagacgcc catatttatg accgccacat tcctatggta gaagaacttc tgaaaaaaga
     2941 gcctttgccg ggaccaactt tatgggtcga tccggaggta aaagatttct accagtttac
     3001 aacagacagc ttcaagctgg aaaattacca gtatcatccg tttgatcata aagttcctgt
     3061 agcgatctga tcagcaattt aaaaatacgc cgttaggctt acagtctaaa accgccctgc
     3121 catcttttac attcagatga cagggcggtt ttctggtttt ctattatcgt tactgtttaa
     3181 ccattacatc ttactatccc aactggattg cttttcttaa tccaaaggaa tagatccatt
     3241 tttcttttac tttctttcct gtgagctggg tcagggctct tccatagtaa tctaactgca
     3301 cctgataccg ttcttttaac agttcttctc ctttttgtgt atcttctttg ggtacataat
     3361 ctgttttgta gtcaatgagg ataaatccat cggcttcttc tatataagcg tcgatcactc
     3421 cctggatcag caccagttcc tcagactgca gcccttttcc catttcactg gcagggatac
     3481 ctaccataaa ctggcgctcc ctgtaaagtt ttccttcctt ttcagcctgt ttcatccgct
     3541 tacccagagg actttccaga aatttccata tatcccatgg aaagatggac tcataggctt
     3601 cttctgtaag aaagcctcgc tgaaacaggt ctaacagatc cccttccagt tttctctggt
     3661 ctgtaacagc aagcagatcc agacattcta aggcacggtg ataagccgtt cctctgaatg
     3721 cacctttctt tccagtttct gtctgttttt tgccatcttc tgcttcactg ctttccggtt
     3781 ccatttcatg ttcgtggtcc agtctgactt cggacaggtc ctttcgttct gttccgatat
     3841 tttcttcttc acgcccgatc tgactctgtt ttttcagttc cgacacggac atctgtgtat
     3901 acaggcctgt atcttccaga taaggatact cataacttag cgcctgatcc agctgctctt
     3961 tcatctgttc atcgtaaacc tgttttgtat ccagttttaa caactcttct tttacatttt
     4021 tcttttccag ctgccgctgc agttctctgc cgatcagatg ggtaagctgc acctgtttta
     4081 cttccatatg tccagcggga actgctgctt ttgccataag cacccagtct aaaaatgaat
     4141 ttgccccgga caggactgta taggggatct gtccatctgt taaggagagc tgatcccatt
     4201 tttccagttt attttccagg gaacggtctg ttcctgtcat gaccagcact tcctttgcac
     4261 gggtcatggc aacatagaga actctcaact cttctcccat agactccagt tccatccgcc
     4321 gtctgatggc cattttttta agagtaggcg ccttggtgcg caggttcaga tccatttcat
     4381 ctgcagctgc ccccagatct gcatctaaaa gcagctggcc ataggcatcc tgcttgttaa
     4441 atttctttcc cattccggct aaaaatacaa ctggaaattc cagaccctta cttttgtgga
     4501 tactcatgat ccgcacagaa ttgctttcat ctcctaaaga ggcttctcca aagtctgtat
     4561 catatttttt cagcttttca atgtatctta caaaatgaaa taagcctctg tagctggtgg
     4621 aagcataggc tgccgctttt tctgccagca tatccagatt ggcctttctt gtctctcctg
     4681 ccggcatgga ggacacataa tcataatatc ctgttttccg gtagatctga taaagaagct
     4741 catggacagg cagataccgg ctgtcctggc gcagttcttc tgtaaaagct gcaaaatatt
     4801 ccagtttttt ccagatagat ttagcggttg ctttcgggat ctctacctgg ccgatccgga
     4861 tctgctctgt ttccggatct ttcttttcat cttcatccgg attttcatca gaaacagcct
     4921 gtggatagat ctcctcccac aatttccagg caccatatac cccacggtcc tgttttttct
     4981 ggagataccg tttataggca gcgatcatcc atgccatttc atcatccgtc atcccgacca
     5041 taggggagcg caaaacagct gccataggaa tatcctgcat aggattatcg atcacagaca
     5101 gaaggccgag tacggtttcc acttccgctg ttgcaaaata tccggtcctt gtctgggcag
     5161 ttgccggtat tccttcattc atcagaacat ttacaaagac ctcagaccag ccactcatac
     5221 tgcgcagcag gatcaccata tccttatagc aggcttttct gtaactctgc ccgtccttat
     5281 cccacacata cagaccgttt ttttcatctg taaagctttt gatctgttct gcgatcattt
     5341 ttgcttccag ttccctggct gtataatctg ctgcctcttc gtctagctgc ttaaaagctt
     5401 ctgttcccgt atctgccaca agaaactgga ccgggatccc cgcttttttc tctgtctctt
     5461 caaacgcagc tcccggataa agggccgttt cttctgtata acggatgcct cccagatttt
     5521 tcgtcatgat ctgatagaaa atatcattga tacaggtaag tacactggca cggctgcgga
     5581 agttctgctg cagctcgatc atctgatgac tgctttcttc cctgctgtaa gtatcatatt
     5641 tttccataaa taactccggc cttgccagac ggaaacggta gatactctgt tttacatcac
     5701 cgaccataaa tacatttggc tggcccagcc gttccctgga aatactctgg atcaatgtct
     5761 cctgtaccag attgctgtcc tgatactcat ctaccagtat ttcttcgtat tgtccgctta
     5821 actccccggc tgcctgtgaa ggaacccggg tcccatcttc ctcttctact gtaagcacct
     5881 gcagcgtaag atgttccagg tcattaaaat ccagcacatt ccgctctctt tttgcttctg
     5941 tgtaggctgc gtcaaaacgc tttaccatat ctaaaagaac ctggatcaca attctggtat
     6001 ttctgatccc ctctattgct gcttccggtg actggcttcc ataggtatcc ctgcattttt
     6061 ctgccgcttt tttcaccctg atgcgcagat cacttacata ggcctttttc tccggatcga
     6121 tctctttgct tctggctgct gccagacggt caaaggtcag ttctgatact gcatgataaa
     6181 aagcctcata agagccggaa gctgctgctt ccctgatctt ttttaaccgg ctcacatcat
     6241 tcatcagcat cggttcatag acaaaaggac cattttcttc ccttgccacc tccagggctt
     6301 cttctgcctg ggcagccagt tctcccatct gaaggctgat atcccttaaa agaaatctca
     6361 tccactcact ggagtccaga tccccctctg cttctttttc catctgctgt ctgcactgat
     6421 ctaaccactg gtccggccag ggatggctct gggaaaaggt ccatgcctga aggatcacat
     6481 cttccattgc cttatctgat tttccacttc caaactgctc cgaaaactgt aaaaagccgt
     6541 catctgcagt ttcgtaacat tcttctaaga cctgctccat tacatcccct tttaaaagag
     6601 tcagttctcc ttcatctcca atgcggaaag ccgggtcaat atccagtgca ttataatggt
     6661 tccggatcag atccaggcag aaactgtcaa tggtcatgat ctttgcctgg gggatcaggg
     6721 ctgactgaag ccacaggtgc tcatttccag gatcttcttt cagctttttt tccacagctg
     6781 ccccgatccg ctcccgcatc tcagaagctg ccgcattggt aaaagtcata acaagaagct
     6841 ggtcaatatt taaaggatgg gttccttcac tgatcatgga aatgatcctt tcaaccagca
     6901 ccgctgtttt tccgcttcct gctgctgcag ataccagcag gtttctgttt ctgctgtcta
     6961 taacctgctg ctgtttttct gtccatttca tcttttattc atccccttcc ccgtttttat
     7021 cttccttcat gccacccttt tcctcgattt cctgccatac ttcctccggc ttcatagagt
     7081 gaagccgcct gaaaccatag cccgccgttt tcgtatcaaa accgcagact gcatggtacg
     7141 ggcagtaatc acaggccgtt ctgtctccgt ctttataagg tgccagtcca ttttcacctt
     7201 ttaaaatagc ctgtccggac tgttttaacc gttttcccac aaatcggcga agagcctgga
     7261 aacgtgcttc tcctgccaca ctggattttg cttcctgtac ataaccgtct tttacagcaa
     7321 cgggtattac gtctgactcc ttctggatct cacgatccag gtgatggatc acctccagat
     7381 cgctgtttac cagtccgttc atacgaagct gctttaagat ctgtttttcg atctcctccg
     7441 gtgtcatttc cccttctttt ttgatcatgg ggtctttgat attgtaatag aaaattcctg
     7501 ccggaactgc ggttttttcc ggatttttcc gttcttccat ttccagggcc gcatccatat
     7561 ataccaccag ctgaagctgc aggccatagt acagtgctgc cagatcaaag gaggtatttc
     7621 ctgatttata gtcaatgatc tttacataca catgtttttc atcttcacag agatccagac
     7681 ggtcgatccg gccctttaac agcagttctt catcctctga caggcggatc ttcattgcct
     7741 ttaaattgtc aatagcagaa aaggacactt caaaaccgga gggcacaaaa tcccctttct
     7801 tcacctgctc tgccagcgcc cagatggtac ggtcggtaat ccgctccaca cgctttgcca
     7861 gataagtatt tctggcagaa ctggacataa tggtatttcc gtactgggat gtcacctggt
     7921 ccacacattt tttcaccagt tcttttcgtt cttcttctgt aatattgctc cagtccatcc
     7981 cattttcata gatcacctca aaacactggt ctaaggactg gtggaacaga tttcccatat
     8041 ccactgcctc taactggtac tcctgacgtt ccattaattc cagaccgtat ttcaagaaat
     8101 gagcataggc gcaggcagca aactgctcta acctggtaac acttccctga agtttctgtc
     8161 cgtaaagggc tctggctgcc gcacggccga ttcccttatc ttcataaaca aaaaaggctg
     8221 catctgtcag tttttctacc agtttcctgt actcttcctg tccataaaaa cgacggaaca
     8281 attccagaaa ggttccctgg gcttctttta actgttccag atttttatct cctgcttccc
     8341 gcagtcctgc catcagcttt tttaaaccat cttccgctgt ccataccggc cattcctctt
     8401 cccgcttttc ccttggcacc atttccggga acagtctgcg gatctccccg atcagactgg
     8461 aaggatggca gcttttgccc tgaccattca tagccgcata ggtgatcacc agctgcctgg
     8521 aaggcttgga aagcatgaga tacagataga atttctggat acagccctct tcccttgcag
     8581 taggtgcaag ttccatatga aggctcttaa acacttctct gtccccgtca tttaacaggc
     8641 ttcctcccgc ctttctctga ggaacaatgc cttcgttcac acctgtgaaa aataatactt
     8701 ttacagactc cagacggctt cgggtaatat ctccgatcat cacctggtct gctgttgcag
     8761 gaaggacacc gatcttgatc tcttcaaaac ctgcatccag gatctggata taatttttca
     8821 tatccgcctt ttcgtcccct aaaaggccct ctaaccgttc taacagctcc atgacccgct
     8881 catagacctg gctgtattct ctggcccggt tctcatctcc cggttccttc cgctccagaa
     8941 aatagttgct gaaactttcc agcttttctt ccagcttcat gctttcaagg acctggcgca
     9001 aaaccgtggt aacagaagaa atggtggcat tttcctcttt aaaagcctgg cgcaggacct
     9061 ttaaaggttc taacatccac tgacggtact ggttcagttc ttccagactc agccgttccc
     9121 cgcctctgca tggcttttcc cagatcatat cccagttttt ccagcctttg atccccaggg
     9181 ctcttgcata gttttccatc cggtctgtaa gaacctgtgc ttcctccctg gaataggttt
     9241 catcatagac aaaacctgtt ttcagataac ggaaaatgct ttcgtaagaa gcatctttta
     9301 caccatctaa cgcagccctt ataagctcga taaaaggatt ttccagaata ctctttttat
     9361 catccagaaa atacgggatc cctgcctcat caaaccggtg ggcgatctct tttccataag
     9421 aagccagatc tcctgtgact actgccagat cccggtagcg catgccttcc ttctgcactt
     9481 tttcattaat gcagctgcac acatattccg tctcttctgc cggatttttt ccccggaaga
     9541 tctgaatgtc ctgaggatca tttttccata ttttcccatt ataacggtat aaattctgct
     9601 ccaaaaaatc cagctggggg ctttccttaa aacgccaggc cgggcggtta tcacaccata
     9661 cttcctttaa aacaggaata tgatgttcct ctgccatttt tctcaacctg catacagtat
     9721 gccgtcccat gtaaaataaa tgctgcatgg tacattcttt ataaagcatt tcccttggat
     9781 ctgcagtaac acagcagatc acctccctgc tgtggatcat aaacagttct gcaagacggt
     9841 actgcaccgg cgtaaaaccg gtataaccat ccaaaaggat cacgctgttt tttaaaggtt
     9901 cccactgtgg cagttttctg cacagaatat ccaggatctc ttctgccgta atataatgag
     9961 actcaatata atctttaaat cccccataaa cggtcttcag atcccccagt ttatctttta
    10021 acagtacacg gtctgtttct tcctctacca ttgccaggtc ttctacgctt accccgtact
    10081 gacaaagctc cgagatctgg gattttaact ggttgataaa gcctggctgt tccagatggc
    10141 ctccgtacag tttcagttct tttttcagtt ttcccgctac ctttcggatg accatggatt
    10201 ttcccatatc atctaaaacc tggggcagct gtactcccag atcttcaaaa accctgtatg
    10261 caagacgttt aaagcttaaa acatcaatat taagaagtcc atgtctgggg tgtaagcgca
    10321 ccagttcttt ctgggtctgc atggtatact gttccggcac aataaaaaga tactgtatgt
    10381 ccggatgttc ttcggactct tttatcacct tttcgtaaag ataccttgtt tttccggagc
    10441 cgctgccccc tgcaataaac tgcaatgaca tattttcacc tttcctttca tatgctgtaa
    10501 caaattctac cagaggtcct gatcatgagc tccaag
//
LOCUS       DBMDUA010000094        10478 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3105_length_10478_cov_2.624169, whole genome shotgun sequence.
ACCESSION   DBMDUA010000094 DBMDUA010000000
VERSION     DBMDUA010000094.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10478)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10478)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10478
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3105_length_10478_cov_2.624169"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="ACZPCL_08190"
     CDS             complement(<1..706)
                     /locus_tag="ACZPCL_08190"
                     /inference="COORDINATES: protein motif:HMM:NF016874.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HIR1162480.1"
                     /translation="MQGPHGKEAPYKDLPFSPCIIASTKIAEGDYGDCLKMLMTDIEE
                     EKKYKGLRGYSVDYTAAKTYKSDRPVVYLAGPERYDKDAKELYKKRKELCASYGLQAF
                     TPADWAEGVEKMDTDCPYTAAANQFDSWQQHVRNCDAIIADLNDYRGYECSNDVAFEC
                     GMAFQLGKKMYGYVDDIRPAKEKVPNLGPEHEYRDMTGANVEDFNYPVNLMFSCSMDI
                     REGKFEEVIKEIAKDLY"
     gene            complement(672..854)
                     /locus_tag="ACZPCL_08195"
     CDS             complement(672..854)
                     /locus_tag="ACZPCL_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1162481.1"
                     /translation="MAVAYAIDESLIETFEGRAGIETKDGLTLGNTVVDYRYHHVWED
                     LPKVKIARTARKRGSL"
     gene            complement(912..1109)
                     /locus_tag="ACZPCL_08200"
     CDS             complement(912..1109)
                     /locus_tag="ACZPCL_08200"
                     /inference="COORDINATES: protein motif:HMM:NF014009.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HIR1162482.1"
                     /translation="MEESGVKEGTCVVYTPHTTAAIVYNSGVNLVAIGLDVATHFDVN
                     LTENDIAALDKSDKMEAGFLR"
     gene            complement(1219..1812)
                     /locus_tag="ACZPCL_08205"
     CDS             complement(1219..1812)
                     /locus_tag="ACZPCL_08205"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase family protein"
                     /protein_id="HIR1162483.1"
                     /translation="MDEAMDMRFDMVAQGKALGELLPDMCRQVYDIAEKWKNMEAYDF
                     VGTGFDYAAAWFGHAKVFEALGKYAMHINSEEWLHMNFFMRNVDKIGTIIVANTTNPA
                     MSRNKELIKFAHNLTRPLMVITDGGKEDFGVDTNYVKVPKTKYAINMPLTQFAPICLL
                     CGYIMDMIGEEDGRGCKGVWKIADGARCIRESEMIVQ"
     gene            complement(1880..2683)
                     /locus_tag="ACZPCL_08210"
     CDS             complement(1880..2683)
                     /locus_tag="ACZPCL_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIR1162484.1"
                     /translation="MEKNGKTFIKGLVIGATMTVPGVSGGSMAMVLGIYDRLLKHVSE
                     ITKYPKESLTFLLWFVAGAGSGAFLLSGGISWLLTTRAEGVLRFFFLGAVSGGIPMIL
                     KSASVRRIRGRELICILTGILTALLIALIPQGIFAPGTENTPMNLLFRLAGGFIIAVA
                     LVLPGISASQMLYMLGIYESTLKAISSLDILALLPLAVGGILGTYLTARILEQLIKKH
                     REDTFLMILGFMLASLPELLPGHLAQENLLFCVMALAAGFIGVYFVTLK"
     gene            complement(2841..3557)
                     /gene="deoD"
                     /locus_tag="ACZPCL_08215"
     CDS             complement(2841..3557)
                     /gene="deoD"
                     /locus_tag="ACZPCL_08215"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR1162485.1"
                     /translation="MGTPHNQAEKGEIAKKVLMPGDPLRAKFIAENFLTDAKLDNEVR
                     GAYAYTGTYKGEKVTVMASGMGMPSIGIYSYELFSVYDVDQIIRIGSAGAYVPELNLG
                     DVVLEESAWSESTFAHTQNGYDKDTIYPDPELNAHILEAAKRNHITVKPVKVHSSDVF
                     YTEPNVDGYKEISAKHGCACVEMESFALLHNANVLHKKATCMLTISDSMPKNEHATAA
                     ERQTSFTDMMTVALEACLDQ"
     gene            3685..4413
                     /locus_tag="ACZPCL_08220"
     CDS             3685..4413
                     /locus_tag="ACZPCL_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1162486.1"
                     /translation="MNINKFNYPFTLKQKSFLENFFKFAPPYIQTVLMLRSYKKGSRL
                     VAAGDSCAHVYILLKGRLEAVEEHVANIPYHFTEIRALDIVGDYEFFTEKQPRLVTLT
                     MLEDSLCLVIPAAEYMTWLRSDSHALFLRSRMIITQLVAQAQFQRQNLFADNRTRMLV
                     FLKEQVTQAVNDSTHTENSNGNPNPGSVYPIRIPFTRPEIAAHLGCSVRTVNRTVQEL
                     VDEEIIHLNKGKIWISEKQASTFL"
     gene            complement(4568..4819)
                     /locus_tag="ACZPCL_08225"
     CDS             complement(4568..4819)
                     /locus_tag="ACZPCL_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162487.1"
                     /translation="MLSVSTMALAAMIGSACVPNANQACNVNSCTYDQANCIQAECAA
                     NGGQDLNCLLNGLGACLQDGCQNGNVRPAGVSGLGSCRR"
     gene            complement(4969..7410)
                     /locus_tag="ACZPCL_08230"
     CDS             complement(4969..7410)
                     /locus_tag="ACZPCL_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR1162488.1"
                     /translation="MSILDEMKVRRLYCDGGMGSLLQAAGLAAGELPERWNISHPEVI
                     TDVHRKYLAAGADIMTTNTFGANRLKYDKDGELKAIVEAAIANARKAVEEAGRGYVAL
                     DLGPTGKLLKPLGDLPFETAVSLYKEVVSIGAAAGADLVLIETMSDSYELKAAVLAAK
                     EAGINPETGVRLPIFATVIYDEKGKLLTGGNVESTVALLEGLGVDVLGVNCGLGPEQM
                     KGIVKDILEVSSTPILVNPNAGLPRSENGKTVYDVDPKDFAAVMEEIVRMGAVITGGC
                     CGTTPDHIHAMVELTKDIPVQMPEKKHRTVISSYSQAVVFDKKTIIIGERINPTGKSK
                     FKQALRDHNLEYILREGVTQQDNGADVLDVNVGLPEIDEPSMMEDVVKELQAVIDLPL
                     QLDTSSAEAMERGLRVYNGKPLINSVNGKKEVMEQIFPLVAKYGGVVVGLCLDEDGIP
                     ETAEGRIAVGKKIIDTAAAYGIGPEDIILDGLCMTVSSDSQGAIVTLETLRKIRDELG
                     GKSVLGVSNISFGLPQREIINGAFFTMAMESGLSAAIINPNSEAMMRAYYSFNALMNL
                     DPQCSEYISIYSGQTAGLGQTIGKGGNGAGGSGAAGNGGAGSAGSLGNAIERGLKEVA
                     VESVTALLKEKEALDVINEEIIPALDHVGKGFEKGTVFLPQLLMSAEAAKAAFEVIKN
                     AMAGSGETQEKKGTIILATVKGDIHDIGKNIVKVLLENYSYDVIDLGKDVPPETVVEE
                     AVSRHVPLVGLSALMTTTVPSMEETIKQLRESAPWVKVMVGGAVLTQEYADTIGADAY
                     CKDAMASVHYAETVI"
     gene            complement(7478..8128)
                     /locus_tag="ACZPCL_08235"
     CDS             complement(7478..8128)
                     /locus_tag="ACZPCL_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HIR1162489.1"
                     /translation="MELADINRREVLRYLGYKNQEADENVSRIVEDCLELLWKNVSPK
                     YRVREFPLTLGADNRIDGEVFQTKSRNLSVNLKDCEKILVFAATLGVGADYLIQKYNR
                     LEMSRAVILQAASAAMIEEYCDQVCEGLKKEYEEKGLYLRPRFSPGYGDFSLSCQSAV
                     LDALDAGKHLGIKLTDSFLMMPSKSVTAVIGVSQKPHRCDVKGCEACGKTDCLYRR"
     gene            complement(8250..9110)
                     /gene="metF"
                     /locus_tag="ACZPCL_08240"
     CDS             complement(8250..9110)
                     /gene="metF"
                     /locus_tag="ACZPCL_08240"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HIR1162490.1"
                     /translation="MKIKDILAEGRPTLSFEVFPPKTADAYESVEKAASKIAQLKPDF
                     MSVTYGAGGGTSDYTVKIASTIKEEYGVTPLAHLTCVSSTREKVHHVLEELKAHKIEN
                     VLALRGDIPQDGKTPEVYHYASELIREIKEAGDFCIGAACYPEGHPESSNKSVDMDYL
                     KQKVDAGCDFVTTQMFFDNSLLYSYLYRIREKGITIPVIAGIMPVTSGSQLKRIAQMS
                     GSYMPARFLSLVDKYGDNPAAMKQAGIAYATDQIIDLLANGIKGIHVYTMNKPDVAAQ
                     IQSNISELLK"
     gene            complement(9300..>10478)
                     /locus_tag="ACZPCL_08245"
     CDS             complement(9300..>10478)
                     /locus_tag="ACZPCL_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR1162491.1"
                     /translation="SLPHRMLKCIRCIRLRTTIINFYEMMPEKFNNKTNGITQRRFLL
                     HGNPLLADWVTDKIGDEWITNLPHIKELAAFVDDKECQKEFLDIQYKNKVRLAKYIKE
                     HNGIDVDPNSIFDVQVKRLHEYKRQLMNILHVMYLYNQLKDNPNMDIVPRTFIFGAKA
                     AAGYRRAKLTIKLINAVADVINNDKSINGKIKVVFIEDYRVSNAEIIFAAADVSEQIS
                     TASKEASGTGNMKFMLNGALTLGTMDGANVEIVEEVGQENAFIFGLSADEVINYENNG
                     GYNPMDIFNNDPQIRRVLMQLTNGYYSPKDPQMFKEIYDSLLNNCGGSRPDTYFILKD
                     FRSYAEAHEKIDKAYRDRQWWAKAALINVSHSGKFTSDRTIEEYVRDIWHLKKVTLEQ
                     "
BASE COUNT         2648 a         2649 c         2027 g         3154 t
ORIGIN      
        1 tatatagatc ttttgctatt tctttgataa cttcttcaaa ttttccctct ctaatgtcca
       61 tggaacagga gaacataaga ttgaccggat agttaaaatc ctctacatta gctccggtca
      121 tatccctgta ttcatgttcc ggccccagat taggaacctt ctcctttgcc ggacggatat
      181 catctacata gccgtacatt tttttgccta actgaaaggc cattccgcac tcaaaagcca
      241 catcattgct gcactcatat ccccgataat catttaagtc tgcaataata gcgtcacagt
      301 tacgtacatg ctgctgccag ctgtcaaact ggtttgcggc tgctgtataa gggcagtccg
      361 tatccatttt ttccacgcct tctgcccagt ctgccggtgt aaaagcctgc agtccataag
      421 aagcacataa ctcttttctc ttcttataga gttctttggc atccttgtca taacgctccg
      481 gcccagccag ataaactacc ggacggtctg acttgtaagt ctttgcagcg gtataatcca
      541 cgctgtatcc ccgcagtccc ttatatttct tctcttcctc aatgtctgtc atcagcattt
      601 tcagacaatc gccgtaatcc ccttctgcga tctttgtaga agcaatgata catggactga
      661 atggaaggtc tttataagga gcctcttttc cgtgcggtcc ttgcaatctt aactttcgga
      721 agatcttccc atacatggtg ataacggtag tcaaccactg tatttcccag tgtaagacca
      781 tctttcgtct caattcctgc tcttccttca aaggtttcga tcagtgattc gtcaatggca
      841 taggcaacag ccatacagtc aatgaccgta caataagcct caaaacctct tctattgaca
      901 aatcggatag cctagcggag aaatcctgct tccattttat cacttttatc caaagctgca
      961 atatcatttt ctgtcaaatt tacatcaaaa tgggttgcaa cgtccagacc gatggctacc
     1021 aggttcacac cggagttgta tacaatcgct gctgtagtat gaggtgtgta caccacacag
     1081 gttccctctt ttacaccgga ctcttccacg atctttttta catcagacgt gatctcaaaa
     1141 atatcatttt tttgtagaaa tatttatctt ttttaacata atgttcccct ttgttttctc
     1201 ttctagtgca ttcgtactct attgcacgat catttcgctt tcgcggatgc atcttgcgcc
     1261 gtctgcgatc ttccatacgc ccttgcagcc tctgccgtcc tcttctccga tcatatccat
     1321 gatataaccg cataacagac agatcggcgc aaactgggtc agtggcatat taatggcata
     1381 ttttgtctta ggaaccttta catagttggt atctacgccg aaatcttctt tacccccatc
     1441 tgtgatcacc attaacgggc gtgtcagatt atgggcaaac ttgatcagtt ctttgttgcg
     1501 gctcatggcc gggttagtcg tgtttgcaac gataatggtt ccgatcttgt ccacatttct
     1561 cataaagaaa ttcatatgga gccattcttc tgaattaatg tgcatggcat attttcccag
     1621 tgcctcaaat acttttgcat gtccaaacca tgcagcagca tagtcaaagc ccgttcctac
     1681 gaagtcataa gcttccatat ttttccattt ttcagcaata tcgtaaacct gtctgcacat
     1741 atcaggaagc agctcaccca gagcctttcc ctgtgctacc atgtcaaagc gcatatccat
     1801 ggcttcatcc atggtgtagc agcctcttaa aggccattga gtattccagc actacctggc
     1861 ttggtgactt tacccgcttt cattttaatg taacaaaata aacacctata aaacctgctg
     1921 ccagcgccat cacgcaaaac agcaggtttt cttgtgctaa atgacctggc agaagttcag
     1981 gcagcgatgc cagcataaag cccaggatca ttaaaaatgt gtcttccctg tgctttttga
     2041 tcagctgttc cagaatacgg gctgtgagat atgttccaag aatcccgcca acagctaagg
     2101 gaagaagcgc caaaatatcc agactgctga tggcttttaa ggtactttca taaattccaa
     2161 gcatatagag catctgggag gcgctgatgc ccggaaggac taaggcaact gctataataa
     2221 agcctcctgc aagccggaaa agcaggttca tgggagtatt ttctgttcct ggagcaaata
     2281 tcccctgagg gatcagggct ataagaaggg ctgtgaggat ccctgtaagg atacatatca
     2341 gttctcttcc cctgatacgc cttacactgg cgctttttaa gatcatggga attccccctg
     2401 atactgctcc cagaaagaaa aaacgtaaaa ccccttcagc tcttgtagtt aaaagccagc
     2461 tgatcccccc ggaaagcaaa aaagccccac ttcctgctcc ggccacaaac cagagcagaa
     2521 atgtgagact ttcctttgga tattttgtta tttcactgac atgttttaaa agacggtcat
     2581 agatccctaa gaccatagcc atagaaccgc cgctgacgcc tggtaccgtc atggttgcgc
     2641 cgatcacaag gcctttgatg aaagtttttc catttttttc cattttatat gatccccttt
     2701 ttgcctctga cacatttgca ttgccagagc ataatttact ctttatggga ttttatggaa
     2761 aattacagga ttttatacat cagacctacg aacctcatgc ttacccgctt tactgcctgc
     2821 tgacgcaggc agaaccttcc ttactggtcc agacaagcct ctaatgcgac agtcatcata
     2881 tctgtaaagg aagtctggcg ttctgctgct gtggcatgct cattctttgg catggaatca
     2941 gaaatagtca gcatacaggt tgctttttta tgaagcacat ttgcattgtg gagcagtgca
     3001 aagctttcca tttccacgca ggcacaacca tgttttgcgc tgatctcttt gtagccgtct
     3061 acatttggct ctgtataaaa tacatcactg gaatgtactt ttacaggctt tacagtaata
     3121 tgattacgct ttgcagcctc taaaatatga gcgtttaatt ccggatccgg atagatggta
     3181 tctttatcgt agccattctg ggtatgtgca aaagtagact cactccatgc actttcttcc
     3241 agcactacat cacctaaatt caattccggt acataagcgc ctgcagaacc aatacgtatg
     3301 atctggtcta catcataaac agaaaacagc tcataggaat aaattcctat agacggcatt
     3361 cccattccgc ttgccataac agttactttt tcccctttat aagtacctgt atacgcatat
     3421 gccccacgga cttcattgtc cagcttcgca tctgtaagga aattctctgc aatgaattta
     3481 gcacgaagag gatcccccgg catcaatacc ttttttgcga tctcaccttt ttccgcctga
     3541 ttgtgtggtg ttcccataat aaacttcctc cttttttatt ttatgtactc ccggagtctt
     3601 tcaatgtacc tatatcaaaa gaccttttga cacttatatt ataaaatgat atcataatta
     3661 aaaaaaggac atgtggcagt tttgttgaat atcaataaat ttaattatcc atttacatta
     3721 aaacaaaaaa gctttttaga gaatttcttt aaatttgctc cgccttatat acagacggtg
     3781 ctgatgctgc gctcttataa aaaaggcagc cgtctggtag ctgcagggga cagctgcgcc
     3841 catgtgtata tccttttaaa aggccgtctg gaagcggttg aggaacatgt ggcaaatata
     3901 ccttatcatt ttacagaaat aagggcactg gacatagttg gtgattacga atttttcact
     3961 gaaaaacagc cccgtctggt aacactgacc atgctggaag actctctctg cctggtgatc
     4021 cctgcagcgg aatatatgac ctggctgcgg agcgactccc acgctctttt tctcagaagc
     4081 cgcatgatca tcacccagct ggtagcccag gcccagttcc agcgccagaa cctttttgcg
     4141 gacaaccgca cccgcatgct ggtctttctg aaagaacagg tgacacaggc tgtaaatgac
     4201 agcacacata cggaaaacag caatggtaat ccaaaccccg gttccgtcta tcccatccgt
     4261 atccccttca ccaggccgga gatcgcagca catcttggct gctcggtacg gacagtaaac
     4321 aggacggttc aggaacttgt ggatgaagag atcattcatt taaacaaggg aaaaatctgg
     4381 atcagtgaga aacaggccag cacttttctg tgatcgataa ctcagcccgc aaaaccaccc
     4441 aacacagttc tttactaatg cataacgtga aagaccgcct gctctttctt ttgaacagac
     4501 ggtcttttct catacttatt tatctatttt tcttttgtaa cattgcttat tgcaaaatcc
     4561 aatgctctta tcttctgcag cttcccaggc cggatactcc ggctggtctt acatttccat
     4621 tctggcagcc atcctgaagg catgcaccaa gaccatttaa caggcagttt aagtcctgtc
     4681 cgccgtttgc agcgcattct gcctgaatgc agtttgcctg atcatatgta cagctgttta
     4741 cgttacacgc ctgatttgcg ttaggtacgc atgcggagcc gatcattgct gcaagtgcca
     4801 ttgtggatac tgataacata gttttgtcct cgctttcatt ttgttacaaa tttatttcat
     4861 ttatgttaca aaactatatt atcacatttt tctggaaaat tacctggtaa taaatggtag
     4921 tttcacgaac cttcgttaat ggcttgcttt tcttttggac tcattacatt atataactgt
     4981 ctccgcatag tgtacagatg ccatagcatc tttgcaatac gcatctgctc caatggtatc
     5041 tgcatactcc tgagtgagca ctgctccgcc taccatgact tttacccacg gggcgctttc
     5101 acgcagctgc ttaatggtct cctccatgct tggaactgta gtagtcatca gggcgcttaa
     5161 gcctactaat ggtacatggc ggcttacagc ttcttctact actgtctccg gcggaacatc
     5221 ttttccaaga tcgatcacat cgtagctgta gttttccaga agaaccttta cgatgttctt
     5281 tccaatgtca tggatatcgc ctttgacagt tgcaaggata atggtacctt tcttttcctg
     5341 ggtctctccg ctgcctgcca tggcattttt gatcacttca aaggctgctt tggctgcttc
     5401 cgcactcatt aacagctgtg gaaggaaaac agtacctttc tcaaagcctt ttcctacatg
     5461 gtccagtgcc gggatgatct cttcattgat cacatccagg gcttcttttt cctttaaaag
     5521 agcagttacg ctttctactg ctacttcttt taaacctctt tcaatggcat ttccaagact
     5581 tcctgcactc cctgcaccgc catttccggc tgcaccggaa cctccggcac catttccacc
     5641 ttttccaatg gtctggccca gacctgcagt ctggccactg taaatgctga tatattcact
     5701 gcactgagga tccaggttca tcaatgcgtt aaagctataa taagcccgca tcatagcttc
     5761 agagttcgga ttgataatgg cagcgcttaa accgctttcc attgccatgg taaagaaagc
     5821 accgttgatg atctctctct gaggaagtcc aaaggagata ttggatacac ctaaaacaga
     5881 ctttccgcct aattcatcac ggatctttct aagagtttca agagtaacga tcgctccctg
     5941 gctgtcagag cttaccgtca tgcaaagacc gtctaaaata atatcttccg gtccaattcc
     6001 ataagcagca gctgtgtcaa tgatcttctt tcctactgca attcttccct ctgcagtctc
     6061 aggaatgccg tcttcatcca gacaaagtcc tacaacaaca ccgccgtatt ttgcaaccag
     6121 cgggaagatc tgttccataa cctctttttt accgttgaca gagttgatca gaggctttcc
     6181 gttgtataca cgaaggcccc gctccattgc ctctgcactg gaggtatcta actgtaatgg
     6241 cagatcaatg accgcctgca gttcttttac cacatcttcc atcatggaag gctcatcgat
     6301 ctccggaaga cctacgttta cgtccaggac atctgcgcca ttatcctgct gggtcacacc
     6361 ttcacgaagg atatattcca ggttgtggtc acggagagcc tgtttaaatt tggattttcc
     6421 tgtaggattg atacgctcgc caatgataat ggtcttttta tcaaatacaa ctgcctggga
     6481 ataggaagaa atgaccgtgc ggtgcttctt ttccggcatc tgcactggga tatcctttgt
     6541 cagttctacc atggcatgga tgtgatctgg tgttgtacca cagcagccgc cggtgatcac
     6601 agcgcccatt cttacaatct cttccatcac agcagcaaag tcttttggat ctacgtcata
     6661 tacggtcttt ccattttcac ttcttggaag tcctgcattt gggttaacca ggatcggggt
     6721 agaagaaact tccaggatat cttttacgat ccccttcatc tgttccggtc ccagaccgca
     6781 gtttacgccc agaacgtcta ctcccagacc ttctaacagc gctacggtgg actctacatt
     6841 tccgccagta agcaatttgc ctttttcatc ataaataaca gtagcaaaga ttggcagtct
     6901 tactcctgtt tccgggttaa tgccagcctc ttttgcagct aaaacagcag cctttaactc
     6961 atagctgtca ctcatggtct caatgagcac cagatcagcg cctgctgccg cgccaatgga
     7021 aactacctct ttgtataaag atacagcagt ctcaaatggc agatcgccca gtggctttaa
     7081 aagctttcct gtaggcccca gatccagggc tacatagcct ctgccagctt cctctaccgc
     7141 ttttcttgca ttggcaatag ctgcctctac aatggccttt agctccccgt ccttgtcata
     7201 ttttaaccgg ttggcgccaa aggtattggt agtcataata tccgcacctg ctgccaggta
     7261 ttttctgtgc acatctgtaa taacttccgg atgggaaata ttccagcgct ctggcagttc
     7321 accggcagca agtcccgcag cctgcagcag acttcccata ccgccgtcac aatacaggcg
     7381 gcgcactttc atttcatcta aaatgctcat attaaacctc ttttgatcta ttttatctga
     7441 tctatttaag cgtaacagtt cataatgact ctgtccgcta ccgcctgtac aggcagtctg
     7501 tttttccgca ggcctcacag ccctttacgt cacagcggtg gggcttttgg ctcactccga
     7561 tcactgctgt tacggatttg gaaggcatca tcagaaaact gtctgtcagt ttgatcccca
     7621 ggtgctttcc tgcatccagt gcatctaaaa cagcgctctg gcagcttaaa gaaaaatccc
     7681 cgtatccggg actgaacctt ggccgcagat acagaccttt ttcttcgtac tcttttttca
     7741 ggccttcaca cacctgatcg cagtattcct cgatcatagc cgcagaagca gcctgcaaga
     7801 tcactgcccg gctcatttcc aggcggttgt acttctggat caggtaatcc gctcccacac
     7861 ccaacgttgc ggcaaaaacc agtatttttt cacagtcttt taaattaaca gaaagatttc
     7921 tgctttttgt ctggaaaact tccccatcga tccgattgtc tgctcccaga gtaagaggaa
     7981 attcccgcac ccggtactta ggtgaaacat ttttccacaa aagctccagg cagtcttcca
     8041 cgatcctact tacattttcg tctgcctcct ggtttttata ccccagatag cgtaaaacct
     8101 ctcttctgtt aatatctgcc agttccattt tcctgtatgt cctcctgtcc caagccgttc
     8161 actcagattc ccgcactatc gtgctctact gcctgcattc gcaggcggaa ccttcgttaa
     8221 tggcttgttt ttcttttgga ctcattacgt tatttcagca gctctgaaat attgctctgg
     8281 atctgtgcag ctacatccgg cttattcatg gtgtacacat gaattccctt aataccattt
     8341 gccagaaggt cgatgatctg atctgtagca taagcgatac cagcctgctt catagctgcc
     8401 ggattatctc cgtatttatc gactaaagat aagaaacgtg ccggcatata gcttccggac
     8461 atctgtgcaa tacgctttaa ctggcttccg ctggtaacag gcatgatacc tgcgatcaca
     8521 ggaatggtga tgcctttctc acggatgcgg tataaatagc tgtaaagaag gctgttgtca
     8581 aagaacatct gggtagtgac aaaatcacag ccagcgtcca ccttctgctt taaatagtcc
     8641 atatccacag acttattaga agattccgga tgtccttccg gatagcaggc tgcaccaata
     8701 cagaaatccc ctgcttcttt aatctcacgg atcagctcag aagcatagtg ataaacttcc
     8761 ggtgtttttc cgtcctgtgg gatatcaccc ctcagtgcca ggacattttc gatcttatgt
     8821 gcctttaatt cctccaatac atgatgtact ttctctcttg tagaagaaac acaggtaaga
     8881 tgagccagtg gtgtcacgcc atactcttct ttaatagtag aagcgatctt tactgtataa
     8941 tcactggttc cgccgcctgc accgtaagta acgctcataa aatccggctt taactgtgcg
     9001 atcttggaag ctgccttttc tacggactca taagcatctg ctgtctttgg agggaatact
     9061 tcaaatgaaa gggttggtct gccctctgcc agtatatctt ttattttcat aatgtagatc
     9121 ctttctttca taaaacatat cttcaaatgt attctgggga tacgtttcag gaacatattt
     9181 ccagaacaca tttgaagaaa tcatttttca ataaagggat gccgcaaaaa cgcaggcttc
     9241 tgcagcatcc cccataaatc acagttttta ttaccattca tgcgctgcac gaacgggtct
     9301 tactgctcta atgtcacctt tttcagatgc cagatatctc ttacatactc ttcaatggta
     9361 cggtcagaag taaacttacc gctgtgggaa acattgataa gagctgcttt tgcccaccac
     9421 tgtctgtctc tgtaagcctt gtcgatcttc tcatgagcct ctgcatagga acggaagtct
     9481 ttcaggataa agtaagtatc cggacggctg ccgccacagt tatttaacag agaatcatag
     9541 atttccttaa acatctgcgg atcctttggt gaataatatc cattggtcag ctgcatcagc
     9601 actctgcgga tctgaggatc attgttgaag atatccattg gattatagcc gccgttattc
     9661 tcataattga taacttcatc tgcggaaaga ccgaagataa aggcattttc ctgtccaact
     9721 tcttctacga tctcaacgtt tgcaccatcc atggttccca gtgtaagagc gccatttaac
     9781 atgaacttca tgttaccggt accggaagcc tccttgctgg ctgtagagat ctgctcgctg
     9841 acatctgctg ctgcaaagat gatctctgcg ttggatacgc ggtagtcttc aataaataca
     9901 accttgatct ttccattaat ggacttatca ttgttgatca catctgctac tgcattaatc
     9961 agcttaatag tcagttttgc acggcggtag cctgctgctg ccttagctcc aaagataaag
    10021 gttcttggaa ctatatccat gtttgggtta tcctttaact gattataaag gtacataaca
    10081 tggagaatgt tcatcagctg acgcttgtac tcatgaagtc tcttaacctg tacatcaaaa
    10141 atagagtttg gatcaacgtc aatgccgttg tgctccttaa tgtacttagc cagacgtact
    10201 ttattcttat attgaatatc caggaattcc ttctggcatt ccttatcatc tacaaatgct
    10261 gccagttctt taatatgagg caggtttgtg atccactcat caccgatttt gtcagttacc
    10321 cagtcagcta ataatgggtt gccgtgaagc aggaaacgtc tctgggtgat accattggtc
    10381 ttgttgttga acttctcagg catcatctca tagaagttga ttatggtagt tctgagtctt
    10441 atacatctta tacacttgag catacgatgt ggcagcga
//
LOCUS       DBMDUA010000095        10458 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3115_length_10458_cov_3.112388, whole genome shotgun sequence.
ACCESSION   DBMDUA010000095 DBMDUA010000000
VERSION     DBMDUA010000095.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10458)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10458)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10458
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3115_length_10458_cov_3.112388"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2287)
                     /locus_tag="ACZPCL_08250"
     CDS             complement(<1..2287)
                     /locus_tag="ACZPCL_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162492.1"
                     /translation="MGSRRGKEGLLLAVFWVVAIALIGTAANRLILHGPLGGTLQKKE
                     TGRMLMELSVLFLWNLWWLCLAVKSREKRGLAIACLIPGWLLFLWGHRILIPLIGAGV
                     WMGTLAGYGICINNCFLENIGRKNKVVFPVWEKILLGLTTGSAAWMVLVCAVSLTEHG
                     GIGLWRKLALLLALGIAAISLWKNKKNFGRSVDNCLKTEQIADMSGMKKQNVVSKCSN
                     LFRAVMPETVLQAALLAFILTMFFLQAGRLNHELDYDSLHYGLRSAYVLDNGNGIYKN
                     LGLINLVYTYSKGAEVLVLPLSGTPTYGFVLSFTFWTTIVSVVTAAVMGARFSGKTAG
                     FWTGALVSAIPGIMNMAVSAKSDSITLMCQLIIYDFLCLAVVRENIWLIFAVAVYLLS
                     LVYKPTALVFSTALGGVGLVSLLIFKKTGKPEKRAFLTWILPLFTTTGLWYRTWLLTG
                     VPVTSVYANFFEKIGFKVKYPYNFSHVIGDPSVLSRGEKIHRLLERLKGVLISPVGED
                     MEHVIIAWGTVLVTALLLFWIFQVITRFIKHQQISQLDFFDGFLMITLLIGSAASIYT
                     LAQIDGNYFILVYAVAAISTVRITGYWLKTEETENIAAVTTGSAAVETKKILRSGICL
                     LTVPFFLFNTLIASSTSWAGCPGFTSFKLVNRGYYDHRHHTHKSWERRGCKELYSRFT
                     PKNRVVAFGFHPQVLDMACSVQSYYDITGSGGDVYLVKKLAYFEEYLKWAGTEYIYVQ
                     AGYLADQPRATQIIEDMIAEGT"
     gene            complement(2311..3096)
                     /locus_tag="ACZPCL_08255"
     CDS             complement(2311..3096)
                     /locus_tag="ACZPCL_08255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HIR1162493.1"
                     /translation="MSHKHVYAIPAYGDSPYLESCIKSLKEQKVASPVIMCTSTPSPF
                     LGAMAEKYDLPYYIREGKSNIRDDWNYAYSMADAEFVTIAHQDDMYCKNYGEELLKAA
                     EKYPDMTVFTSDYAIVKNKKVITGDKMLWVKRLLRIPLRFHILCGLKWIKIMPLVLGN
                     PICCPATTYSKKRLGEPFVQSEYQFALDWDHMVQLAGEKGRFICVEKPLIYYRVHEGA
                     TTNACIKDNRRAREEAEMFARFWPKAVVKLIMKGYSSAYKEYE"
     gene            complement(3086..3727)
                     /locus_tag="ACZPCL_08260"
     CDS             complement(3086..3727)
                     /locus_tag="ACZPCL_08260"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose-7-phosphate isomerase"
                     /protein_id="HIR1162494.1"
                     /translation="MKELRFLDELVQRYPVLEPVKGDILKAYEIMRDCYEKGGKLMIA
                     GNGGSCSDSEHIVGELMKGFVKRRPVSAELAQALKAADPQMGEKLAENLQGGLPAIAL
                     TGHGALTTAFSNDVNGDMVFAQQLYGYAKKGDVFLGITTSGNSKNVLYAATAAKALGV
                     TVIGLTGRDGGRIKEFSDVSIIVPVQETFKIQELHLPVYHALCLMLEDHFYES"
     gene            complement(3769..4353)
                     /gene="gmhB"
                     /locus_tag="ACZPCL_08265"
     CDS             complement(3769..4353)
                     /gene="gmhB"
                     /locus_tag="ACZPCL_08265"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HIR1162495.1"
                     /translation="MEHTLNKVVFLDRDGTLNEEVNYLHKPEDMKLLPGVPQALKKLK
                     DAGYKLIVVTNQAGVARGYYTEEDVKILHQYMNQLLEKEGAEIDAFYYCPHHPVHGIG
                     AYKKVCNCRKPGTGMFEMAEKDFPVDRAASYMIGDKLLDTEAGQKFGVTSILVGTGYG
                     AELHKEQQENGEKPPYVHYAADLEQAAQWILERK"
     gene            complement(4391..5464)
                     /locus_tag="ACZPCL_08270"
     CDS             complement(4391..5464)
                     /locus_tag="ACZPCL_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP family kinase ATP-binding protein"
                     /protein_id="HIR1162496.1"
                     /translation="MVIRGRAPLRVSFGGGGTDVAPFCEQQGGAIIGSTINKYAYCSI
                     VPRKDDQIIVHSLDFDMTVKYNTNENYVYDGRLDLVTAALKAMDIKQGCEVYLQCDAP
                     PGSGLGTSSTVMVAMLTAMARWKGIMLDAYAMADLAYEVEREDLKIDGGYQDQYAATF
                     GGFNFIEFHGRNNVVVNPLRIKKDIIHELQYNLLLCYTGKIHVSANIIKDQVQNYEKK
                     DAFDAMCEVKALAYAMKDELLKGNLHSFGKLLDYGWQSKKRMSNKISNPQIDELYAEA
                     LKAGALGGKLLGAGGGGYLLMYCPYNVRHKVAARMEAMGGQLADWNFELRGAQSWVAD
                     EDRWQYDKVKVQMPNGEYCFNLK"
     gene            complement(5500..6207)
                     /locus_tag="ACZPCL_08275"
     CDS             complement(5500..6207)
                     /locus_tag="ACZPCL_08275"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1162497.1"
                     /translation="MQAILLAGGLGTRLRSVVSDRPKPMALIEDKPFMEYVTRELVRS
                     GITEIIFAVGYKGSMVEEYFKDGSEFGFQASYAYEETLLGTAGAIKNAGKFITEDRFY
                     VLNADTFYQIDYTRLAKQQDDQNLDMALVLRQVPDVSRYGKAILDENGILTGFNEKTA
                     DPVPGTINGGIYLMKKSLLEEIPEGKVSLENEMIPKWLSEGKKLGGFVNDGYFIDIGI
                     PEAYFQFQEDVKTGVVH"
     gene            complement(6402..10316)
                     /locus_tag="ACZPCL_08280"
     CDS             complement(6402..10316)
                     /locus_tag="ACZPCL_08280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1162498.1"
                     /translation="MNDISYEILDLFKKYGADKEGILKILEADSRPEVLYALSDIRRN
                     LMDWMDFSGQEHVLQLGSDYGVITGLLAERCASVAVVDERDENLVVNQKMNEAHTNIS
                     YMKAADFQKMEAVEKEGKYDLIVLRADRPDMDIKECFTRLSSYLSENGKLIFACENAL
                     GLNFLSGAVHDEDETAFTKGELEEALKEAGLSKVEFYYPMPEYKRAVSVYSDRYLPGK
                     GDIPHVTAVYDRQRWACIHEDEISDKLVQEKAFGLFSNAYLAVASKGAESFKTVFAKY
                     NSTRKEEFQIRTAILEENGKRYVEKTPLTEKAAGHISSMSEKYEKLSKVNPGVKVLAP
                     VYSEDKKAVRFEFLKGRTLAEILGEQIRGKKAPVEALKAAIDQVFGQAVLKNEPFAPT
                     DPFKEVFGDSEDVLSLQDTSYEVSNIDGLFENLMETEDGLYCMDYEWVFDFPIPSGFV
                     RYRNLVYFYYKYEGLMEYENATQFLKEFGMDEKLAETYASMEVAFQTWVHGDGTQGYL
                     GNYKQRLVTLEELKAQEKELAQARERINQLQAEVEERNIQVKKDQEILRLTNNHVTNL
                     EVMIKDLRHEVNELGKLATYLNGHEAVIFKMRRKLGEQVNKAFPKGTRKRKLLNYSFN
                     TVKHPVKYGKMYATKEGRNLIEGDFKIGEGYLTGGHLSFPQYENPTVSIVIPCYNQIH
                     YTYACLQSILEFTKDVTYEVIIADDVSTDATAEISRFVDGLVICRNQTNQGFLRNCNQ
                     AAKAAKGKYIMFLNNDTKVTEGWLSSLVNLIESDDTIGMVGSKLVYPDGRLQEAGGII
                     WSDGSGWNYGRLDDPDKAEYNYVKDVDYISGAAILLSVDLWKQIGGFDERFAPAYCED
                     SDLAFEVRKAGYRVVYQPLSKVIHFEGVSNGTDVNGTGLKRYQVENSQKLKEKWADEF
                     KKQCVNDGNPNPFRARERSQGKKVILVVDHYVPTFDKDAGSKTTYQYLKMFLKKGYVV
                     KFLGDNFLHEEPYSTTLQQMGIEILYGDHWATGLWDWPKLNKDEIDVAYLNRPHIATK
                     YVDFIKENTNIKVIYYGHDLHFLRLGREYELTGDINIKREADYWRAIELSMMRKAAIS
                     YYPSYVEINAIHAIDPEIKAKAITAYVYDHFLTNIQEDFAKREGLLFVGGFAHPPNAD
                     AVLWFAKEIFPHIREQLPDIKFYVVGSKVTDEIKALEQPGNGIIIKGFVSEEELANLY
                     ASCKVVVVPLRYGAGVKGKVVEAIYNGAPIITTSTGAEGIPQVEQVLEVEDDPKTFAD
                     KTVALYQDNDRCRQMCEGTQKYIREHFSMDGAWKVIEEDFSR"
     gene            complement(10309..>10458)
                     /locus_tag="ACZPCL_08285"
     CDS             complement(10309..>10458)
                     /locus_tag="ACZPCL_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1162499.1"
                     /translation="SCTGFEQGEHVVYHRLYDVTNITVISNKNTVGVYDMESQVKAEK
                     RNADE"
BASE COUNT         2640 a         2634 c         2047 g         3137 t
ORIGIN      
        1 aggtcccttc tgcgatcata tcttcgatga tctgtgtggc tctgggctgg tctgccagat
       61 atcccgcctg tacatatata tattcggttc cggcccattt taaatattct tcaaaatatg
      121 ccagtttttt tacaagatac acatccccac cgcttcctgt tatatcataa taactctgga
      181 cgctgcaggc catatctaac acctgggggt gaaagccaaa ggctactacc cggtttttag
      241 gtgtaaaacg ggaataaagt tccttacagc ctcttcgttc ccagctttta tgtgtatgat
      301 gacgatggtc ataatatcct ctgtttacca gtttaaaaga ggtaaagccc gggcatcctg
      361 cccagctggt agaggatgcg atcagggtat tgaagaggaa aaatggaact gtcagtaaac
      421 agatgccgga cctgaggatc tttttcgttt ccactgctgc actgcctgtt gtaactgcgg
      481 caatgttttc tgtctcttct gtttttagcc agtatcctgt gatccgcacg gtactgatcg
      541 ccgctactgc atacaccagt atgaaataat ttccatctat ctgggcaagt gtgtaaatgc
      601 tggccgcgga accaattaac agtgtgatca taagaaaacc atcaaagaaa tctaactggg
      661 agatctgctg atgctttata aacctcgtta ttacctgaaa gatccagaat aacaggagtg
      721 ctgttacaag cactgttccc caggcaatga tcacgtgttc catatcttct cctaccggag
      781 agatcagtac tccctttaac cgttccagca gccggtgtat tttttctccc ctggaaagga
      841 cagacggatc accgatcaca tgggaaaagt tatagggata ttttacttta aaacctatct
      901 tttcaaagaa atttgcatat acagaagtta ccggaactcc tgtaagcagc caggttcgat
      961 accacaatcc tgttgttgta aataatggca ggatccatgt taaaaatgct cttttttccg
     1021 gttttcctgt ctttttaaag atcaggagac tgacaagccc cacaccgcct aaggctgtag
     1081 aaaatacaag agccgttggt ttgtaaacca gtgaaagaag ataaacagct accgcaaaga
     1141 tgagccatat attttctctg acaactgcca gacagagaaa atcgtaaata ataagctgac
     1201 acattaacgt aatactgtca cttttagcag aaaccgccat attcataata cctgggatcg
     1261 cactgaccag cgctcctgtc cagaaaccgg ctgtttttcc gctgaaacgc gcacccataa
     1321 ctgcggctgt cactacactg actatggttg tccagaaagt aaaggaaagg acaaatccgt
     1381 aagtcggtgt accggacaag ggaagcacca gtacttctgc tcccttgctg taggtataca
     1441 ccagattgat cagccccaga tttttataaa taccgtttcc attatccagg acataggcgg
     1501 aacgcagtcc atagtggaga gagtcatagt ccagttcatg gtttaaacgg cctgcctgaa
     1561 gaaagaacat ggtgaggatg aaagcaagaa gagctgcctg aagcacggtt tccggcatga
     1621 ctgcacggaa tagattgctg cacttactga ccacattctg ttttttcatc cctgacatat
     1681 ctgcaatctg ttctgttttc agacagttat ccacagacct gccaaaattt tttttatttt
     1741 tccacagaga aattgccgca atccccagag caagcagcaa cgccagcttt ctccagagcc
     1801 cgattccccc atgttctgta agagaaactg cacatacaag gaccatccat gctgcgcttc
     1861 cggtggtcag ccccagaagg atcttctccc agacaggaaa aacaactttg ttttttcttc
     1921 caatattttc caaaaaacag ttgttgatgc aaatgccata tccagccagc gttcccatcc
     1981 acacaccagc ccctatcagc gggatcagta tcctgtgtcc ccagaggaac aaaagccagc
     2041 cgggtatcag acaggctata gccagtcctc ttttttccct gctttttact gccaggcata
     2101 accaccataa attccataaa aacaggacag acagttccat tagcatccgt cctgtttctt
     2161 tcttttgtaa tgttcccccc aaagggccat gaagtatcag tctgtttgca gctgttccaa
     2221 tcagagcaat tgccaccacc cagaatacag ccagcaaaag accttctttc cctcgtctac
     2281 tccccatttt aacttgttcc ttttaactct ttactcgtat tccttataag cggaagaata
     2341 tcccttcatg atgagtttta ctaccgcttt aggccaaaat ctggcaaaca tttctgcctc
     2401 ttccctggct ctccggttgt ctttgatgca ggcgtttgta gtagcgccct catggacccg
     2461 gtaatagatc agcggctttt ccacacagat aaacctgcct ttttctcctg ccagctgtac
     2521 catgtggtcc cagtctaagg caaactgata ctcagactgg acaaatggtt ctcccaaccg
     2581 ctttttgctg taggttgttg ccggacagca gatgggattg ccaagtacca ggggcattat
     2641 ttttatccat tttaaaccgc aaagaatgtg aaaccgaagg ggaatacgta aaagacgctt
     2701 cacccacagc attttatccc cggtaattac ctttttattt ttcacaatgg cataatcaga
     2761 ggtaaataca gtcatatccg gatatttttc agctgccttt aacagttctt ccccatagtt
     2821 tttgcagtac atatcgtcct gatgggcaat agtgacaaac tctgcatctg ccatggaata
     2881 agcatagttc cagtcatccc ggatattgct ttttccttcc cggatgtagt agggcagatc
     2941 atatttttct gccatagctc ccaaaaacgg gcttggggtg gatgtgcaca tgatcacagg
     3001 agatgccact ttctgctcct tcagagattt aatacagctt tccagataag gagaatcacc
     3061 ataggcaggt attgcatata catgcttatg actcataaaa atgatcctcc agcattaagc
     3121 acagggcatg gtatacagga agatgcagtt cctggatctt aaaggtttcc tgtaccggaa
     3181 cgatgataga tacatctgaa aattctttga tccgtccgcc gtcacggccg gtcagtccga
     3241 tcacagtcac acctaatgct tttgctgcag ttgctgcata aagcacattt ttagaattgc
     3301 cggaagtcgt aattcccagg aatacatctc cttttttagc gtagccatac agctgctggg
     3361 caaataccat atctccgttt acatcattgg aaaaagcggt agtcaatgcc ccatggccgg
     3421 tgagtgcaat ggccggaagt ccgccctgga gattttctgc cagtttttcc cccatctgag
     3481 ggtctgctgc ctttaaagcc tgggctagtt ctgctgatac aggacggcgt tttacaaagc
     3541 ctttcatcag ctctcctaca atatgttcgc tgtcagagca gcttccgcca ttgcctgcga
     3601 tcatcagttt cccgcccttt tcatagcagt ctctcatgat ctcatatgcc ttaaggatgt
     3661 ctccttttac cggctcaagc accggataac gctgtactaa ttcatccaaa aatctaagtt
     3721 ctttcatata cgattttctc cttttgccag aacttctttt tccaggtttt attttctttc
     3781 cagtatccat tgtgctgcct gttcaagatc agctgcatag tggacatacg gcggtttttc
     3841 accattttcc tgctgttctt tatgaagctc tgccccatat ccggttccta ctaaaataga
     3901 ggtgactcca aatttctgtc cggcttctgt atcaagaagc ttgtccccga tcatatagga
     3961 agcagccctg tccaccggaa aatccttttc tgccatttca aacatgccgg ttcccggttt
     4021 gcggcaatta cacacttttt tgtatgcacc gataccatgt accggatgat gagggcaata
     4081 ataaaaggca tcaatttctg ctccctcttt ttctaacagc tggttcatat actggtgaag
     4141 gatttttaca tcttcttctg tataataccc tcttgccact cccgcctggt tggtgactac
     4201 gatcagctta tatccggcat ctttcagctt ctttaaagcc tggggcactc ccggaagaag
     4261 cttcatatct tccggtttat gaagataatt cacttcttcg tttaaagtgc cgtcccggtc
     4321 taaaaatact actttattta atgtatgttc cacgatattg tttcccattt ctataacgat
     4381 ttatagtttt ttatttcaga ttaaaacaat attctccgtt tggcatctgt acttttacct
     4441 tgtcatactg ccagcggtct tcgtctgcta cccagctctg ggcgccgcga agttcaaagt
     4501 tccagtcagc taactgtccg cccatagctt ccatacgggc tgctacttta tggcgcacat
     4561 tgtatgggca gtacattaac aggtatccgc cgccgcctgc tcctaaaagc tttccgccta
     4621 acgccccggc ctttaatgcc tctgcataca gctcgtcgat ctgcggattg gagatcttat
     4681 tgctcatacg ctttttgctc tgccagccat agtctaacag cttgccaaaa ctgtggagat
     4741 tgccttttaa cagttcatct ttcatggcat aagccagtgc ctttacctcg cacatcgcgt
     4801 caaaagcatc tttcttctca tagttctgca cctggtcttt gatgatattg gcagaaacat
     4861 ggatctttcc ggtatagcat aacagcaggt tatactgcag ttcatggata atgtcttttt
     4921 taatacgcag cgggtttacc actacattgt tgcgtccatg gaactcaatg aaattaaagc
     4981 cgccgaaggt tgccgcatac tgatcctggt atccgccatc gatctttaag tcttctctct
     5041 ctacctcata tgccagatca gccattgcat aggcatctaa cataatacct ttccaccgtg
     5101 ccatagcagt aagcatagca accatgacgg tagaagaggt tccaaggccg gagcctggag
     5161 gtgcatcaca ctgtaagtaa acttcgcagc cctgcttgat atccattgcc tttaaggctg
     5221 ctgttaccag gtctaagcgt ccgtcataga cataattttc atttgtgttg tattttactg
     5281 tcatatcgaa atccagagag tggacgatga tctggtcatc ctttcttggt acgatagaac
     5341 aatatgcgta tttattaatg gtacttccga taatagcgcc gccctgctgc tcacagaatg
     5401 gtgctacatc tgtgccgccg ccgccaaagc tgacgcgaag gggcgctctg cctctgatca
     5461 ccatagtttt tctcctttcg gacccatgta tgccgataat taatgtacaa ctcccgtttt
     5521 tacatcttcc tggaactgga aatatgcttc cggaataccg atgtcaatga aatagccgtc
     5581 atttacaaaa ccgcccagtt tctttccttc agaaagccac ttggggatca tctcattttc
     5641 aagagatact ttgccctccg gaatctcctc taacaggctc tttttcataa gataaatacc
     5701 gccgttaatg gttcccggca ccggatctgc tgttttttca ttaaatccgg tgagaatgcc
     5761 attttcatct aaaatggctt tgccatagcg ggacacatcc ggcacctggc gcagcaccag
     5821 agccatatcc aggttctgat catcctgctg ttttgccaga cgggtatagt cgatctggta
     5881 aaaggtgtct gcgtttaaca cataaaaacg gtcttctgta ataaacttac ctgcattttt
     5941 aatagcgcct gctgtgccta aaagagtttc ctcataggca taggaagcct ggaagccgaa
     6001 ttcacttccg tctttaaaat attcttccac catggaacct ttgtaaccta cagcgaaaat
     6061 gatctctgta atgccgctgc gcaccagctc cctggtcaca tattccataa atggtttatc
     6121 ctcgatcagt gccattggct ttggtctgtc gcttactaca ctgcgaagtc tggttcccaa
     6181 acctccggct aataagattg cctgcatttt attctcctgt tctgtttttc ccttttttgc
     6241 agtgctaacc tccaaaatgc tacgcatttt ctcggtcgcg ggcattcgcc ctatgaaaaa
     6301 acttctcagc cgtatcttcg gaaagtaaac ttctcagccg tatcttcgga aagtaaactt
     6361 ttctcagttt cgtctgagaa gttttttcgc attgctcatt gctatcgcga aaagtcttcc
     6421 tctattacct tccatgcccc atccatgctg aagtgttccc tgatatactt ctgggttcct
     6481 tcgcacatct ggcggcagcg gtcattatcc tgatacaaag ctactgtctt atctgcaaat
     6541 gtctttggat catcttctac ttcaagaacc tgctctacct gcggtatgcc ttcggcaccg
     6601 gtgctggtgg taatgatcgg tgcgccgttg tagattgcct ctaccacttt gccctttaca
     6661 cctgcaccgt aacgcagggg aactaccact accttgcagg aagcatacag gtttgccagc
     6721 tcttcttcac tgacaaagcc cttgatgatg ataccgtttc caggctgctc taaggctttg
     6781 atctcatctg ttaccttgga accgactaca tagaatttaa tatccggcag ctgctccctg
     6841 atatgtggga agatttcctt cgcaaaccag agcacagcat ctgcattcgg cggatgggca
     6901 aaaccgccca caaacagcag tccttctctc tttgcaaagt cttcctgaat atttgtcagg
     6961 aagtgatcgt atacatacgc agtaatggct tttgccttga tctctggatc aatagcatga
     7021 atggcgttga tctccacata agacggataa taggaaatag ccgccttgcg catcatggaa
     7081 agttcaatgg ccctccagta atcagcctct ctcttaatat taatatcccc ggtcagctca
     7141 tattcacggc ccagacgtaa gaagtgcagg tcatgaccat agtagatcac cttgatattg
     7201 gtattttcct tgatgaagtc tacatatttg gtagcaatat gaggtctgtt cagataagca
     7261 acgtcgatct catctttatt taatttcggc cagtcccaaa gaccggtagc ccagtgatcc
     7321 ccataaagga tctcaattcc catctgctga agagtggtag aatatggctc ttcatgaagg
     7381 aagttatctc ccaggaactt gacaacatag cctttcttta agaacatttt caggtactgg
     7441 taagtagtct tagaaccagc gtctttatca aaggtaggaa cataatgatc cactactaaa
     7501 atgactttct tgccctgact gcgctctctt gcacggaatg ggtttggatt tccgtcattg
     7561 acgcactgct tcttaaactc atccgcccat ttttccttta atttctggct gttttccacc
     7621 tgataacgct ttaatccggt accgtttaca tccgtaccat tggaaacgcc ctcaaaatga
     7681 atgactttgg aaaggggctg gtaaaccact ctgtaaccgg cttttcttac ctcaaatgcc
     7741 aggtcagagt cttcgcagta agcaggagca aaacgttcat caaagccgcc gatctgcttc
     7801 cataaatcta ctgacagcaa aatggctgca cctgaaatat agtctacatc ttttacatag
     7861 ttatattctg ccttatccgg atcatccaga cggccgtaat tccagccgga accatcgctc
     7921 cagataatac cgcctgcttc ctgtaatctt ccatccgggt aaaccagctt ggaaccgacc
     7981 ataccaatgg tatcatctga ctcgatcagg ttcaccaggg aagaaagcca gccttctgta
     8041 accttggtat cattgtttaa gaacatgata tacttgcctt ttgcagcttt ggcagcctgg
     8101 ttgcagttgc gaaggaagcc ctggttggtc tggttgcggc agatcacaag gccgtctaca
     8161 aaacggctaa tctctgcagt agcatccgta gaaacatcat ctgcaataat aacctcatac
     8221 gtcacatctt tggtaaattc cagaatggac tgtaagcagg cataggtata atggatctgg
     8281 ttatagcacg gaattacaat agaaacagtc gggttctcat attgcgggaa agaaagatgt
     8341 ccgccggtaa gataaccttc tccgatctta aagtcacctt cgatgaggtt gcgtccctct
     8401 ttggtggcat acatttttcc gtatttaacc ggatgcttta ctgtattaaa actgtaattt
     8461 aaaagtttgc gttttctggt tccctttgga aatgccttgt ttacctgttc tcccagtttc
     8521 cggcgcattt taaagatcac tgcttcatgt ccatttaaat aggtagccag cttgcccagt
     8581 tcattcacct catggcgcag gtctttgatc atcacttcca ggttggtcac atggttattg
     8641 gtaagacgca ggatctcctg gtctttcttt acctggatat tgcgctcttc tacctctgcc
     8701 tgaagctggt tgatccgctc tcttgcctga gccagttcct tttcctgggc ctttaattct
     8761 tccagtgtca ccagacgctg cttgtaattg ccaaggtatc cctgagtacc gtctccatgt
     8821 acccaggtct ggaatgctac ttccatagaa gcataggttt ctgccagctt ctcatccatt
     8881 ccaaactctt ttaagaactg ggttgcattt tcatactcca tcagtccttc atatttataa
     8941 tagaagtaaa ccagattgcg gtagcggaca aaaccggatg gaattgggaa atcaaatacc
     9001 cattcataat ccatacagta aagtccgtcc tcagtctcca tcagattttc aaagaggcca
     9061 tcaatgttgc tgacttcata ggaagtatcc tgtaaggaaa gaacatcttc actgtcacca
     9121 aatacctctt taaacgggtc tgttggagca aatggctcat ttttaagaac tgcctgtcca
     9181 aatacctgat cgatcgctgc ttttaaagct tctaccggtg ctttctttcc tcggatctgt
     9241 tctcctaaga tctctgccag tgttcttccc tttaaaaatt caaagcggac tgccttttta
     9301 tcttcggaat acacaggtgc cagcactttt acccctggat ttacctttga taatttctca
     9361 tatttttcac tcatggaact gatatgccct gccgcctttt ctgtaagagg cgtcttttcc
     9421 acatatcgtt ttccattttc ttccagaatt gcagtccgga tctggaattc ttcttttctg
     9481 gtactgttgt attttgcaaa tactgtctta aaactctctg ctcccttaga tgctactgcc
     9541 agatatgcat tggaaaacag accgaaggcc ttttcctgca ccagtttatc ggagatttcg
     9601 tcttcatgga tgcaggccca gcgctgtctg tcatatactg ccgtcacatg agggatatct
     9661 ccctttcccg gaagataccg gtcagaataa acagatactg cccgcttata ttccggcata
     9721 ggataataaa attccacttt ggaaagtcct gcttctttca aagcttcttc cagttctccc
     9781 tttgtaaatg cagtctcatc ctcatcatgg acagcaccgg aaaggaagtt taaaccaagg
     9841 gcattttcac atgcaaagat cagcttgccg ttttctgaaa gatatgagga taaccttgta
     9901 aagcattcct taatatccat atccgggcgg tctgcacgta aaactatcag atcatatttt
     9961 ccttcttttt caactgcttc cattttctgg aaatcagctg ctttcatata ggaaatattg
    10021 gtgtgtgctt cattcatctt ctggttcact accagatttt cgtctctctc atctacaact
    10081 gcaacactgg cgcagcgctc tgcaagcaga cctgtgatca cgccatagtc agaaccaagc
    10141 tgcagtacat gttcctgtcc tgaaaaatcc atccagtcca taagattgcg acggatgtct
    10201 gaaagggcat aaaggacttc cgggcggctg tctgcctcta aaatttttaa gatgccctct
    10261 ttatctgcgc cgtatttctt aaataagtcc agaatttcat aacttatgtc attcatctgc
    10321 gttcctcttt tccgccttta cctgtgattc catatcgtaa acgccaacgg tatttttatt
    10381 ggaaataacg gtaatattgg ttacgtcata aagtctgtgg tacaccacat gttctccctg
    10441 ttcaaagccg gtacagct
//
LOCUS       DBMDUA010000096        10248 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3178_length_10248_cov_2.643540, whole genome shotgun sequence.
ACCESSION   DBMDUA010000096 DBMDUA010000000
VERSION     DBMDUA010000096.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10248)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10248
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3178_length_10248_cov_2.643540"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            140..334
                     /gene="thiS"
                     /locus_tag="ACZPCL_08290"
     CDS             140..334
                     /gene="thiS"
                     /locus_tag="ACZPCL_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HIR1162500.1"
                     /translation="MVTINGEEKNAAGKTIYAYLSEEGYDTSRVVVEQNLQIIPQDQL
                     ENTLIQEGDQIEILCFVGGG"
     gene            337..990
                     /gene="thiF"
                     /locus_tag="ACZPCL_08295"
     CDS             337..990
                     /gene="thiF"
                     /locus_tag="ACZPCL_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HIR1162501.1"
                     /translation="MTAIQEKMQALISKEELERAFDARFPREMKEKFSNARVAVAGLG
                     GLGSNIAVMLARSGIGHLLLVDFDTVDVTNLNRQMYMIPHLGRPKAEALPDILCQINP
                     YLEYESVCVKVTPENVKQLFEMYPIVCEAFDKPDQKAMLVRELLGQYPETMVVSGNGM
                     AGLGDTNDIQTRKMMRRLYVCGDQKTDVGNGIGLIAPRVAACAAHQANKVLQLILQS"
     gene            1057..1821
                     /locus_tag="ACZPCL_08300"
     CDS             1057..1821
                     /locus_tag="ACZPCL_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HIR1162502.1"
                     /translation="MEDKLVLGGKEFSSRFILGSGKYNINLIKAAVEQGGAEIITLAL
                     RRANTQEKENILDHIPEGVTLLPNTSGARNAEEAVRIARLSRAMGCGDFVKVEIMRDT
                     KYLFPDNYETVKATETLAKEGFIVLPYMNPDLNVARDLQSAGAAAVMPLAAPIGSNKG
                     LATKAMIQVLIDEIDLPIIVDAGIGKPSQACEAMEMGAAAVMANTAIATAGDVPAMAN
                     AFKLAIEAGRKAYLTGMGRVLARGGSASDPLTGFLR"
     gene            1834..3093
                     /gene="thiH"
                     /locus_tag="ACZPCL_08305"
     CDS             1834..3093
                     /gene="thiH"
                     /locus_tag="ACZPCL_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HIR1162503.1"
                     /translation="MNEENQVYFVDSDKLPEEALKRKHRLEQDPTFRTNHMEYMEGME
                     VSRSDIKDKVEEAMKAFDYDAYTAGDVERALSHETCSIEDFKALLSPAAAPYLEVMAR
                     KAEEITKNHFGNTVYIFTPLYIANYCQNYCIYCGFNCYNNIRRKKLTFEEIEHEMQVI
                     AKTGMEEILILTGESRAYSDVKYIGEAVKIARKYFRNIGIEIYPVNVDEYKYLHECGV
                     DYVTVFQETYNTDKYETLHLMGHKRVWPYRFDAQERALMGGMRGAGFSALLGLDDFRK
                     DALATALHVYYINRKYPHAELSLSCPRLRPIVNNDKINPRDVGEKELCQVLCAYRIFL
                     PFAGITVSSRESAAFRNGITKICATKVSAGVSTGIGDHEEKYEGKEDADVGDEQFEIN
                     DGRSFASMYEDMEKGGLQPVLNDYIYV"
     gene            3318..4337
                     /locus_tag="ACZPCL_08310"
     CDS             3318..4337
                     /locus_tag="ACZPCL_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162504.1"
                     /translation="MKKTGKIWVALITALSLAMPMNVWADTKITSISLKIDSSIEAGD
                     SDCDVDVTTSSRYCSVDDVEVTNEPSDEWKSGARPKIKVTLSSDGDAYFGTGITKSDI
                     SVSGNDATVTSVSRSGKYELNVSLTLEKLERDSSDYDLDVTGLNWDDYDGTASWEEPE
                     DAKRYEVRLYRDGSSVSSAISTTDTSYNFASYFTRSGDYTFKVRAIYNTSNKGDWEES
                     DELSVSSSEAKDIRDNGKSSGNTPSNSIDDDGGPGDTNTSQGAWLKNDTGWWYCNADR
                     SYPVNQWQYINNYWYFFNASGYMVTGWVQWNNVWYYCCDSGEMLTNTRTPDGYYVDGN
                     GAWIQ"
     gene            complement(4367..5428)
                     /locus_tag="ACZPCL_08315"
     CDS             complement(4367..5428)
                     /locus_tag="ACZPCL_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1162505.1"
                     /translation="MAVTIKDVAALAGVSSSTVSRTCKNNPSISEETKEKVRRAMAQL
                     GYEPNFQASNLASQNSRTIGIILPVSAREAYENSFYLEAIRGISQLCNKRQYINTVIT
                     GESEEEVLQVIKTMARSGQVDGFIVLYSRQDDPIADFLYNEGFLYVLVGKAYSHANQT
                     IYIDNDNLLAAQEATEYLYQMGHRRIGYIGSDTSMIFSADRKSGYQTALLQHNILPRV
                     DYCVEMTAAINAGGTSASSTATEKDSAPLRCLLSLPDRPTAVVVSDDILAATLERVCL
                     EMGLSIPEDLSIVSFNNSLFARLASPQLTSVDINSFQLGAEAATQMINHIEDPGLPAT
                     KILVPHHMVERDSCCPPKN"
     gene            5772..7985
                     /locus_tag="ACZPCL_08320"
     CDS             5772..7985
                     /locus_tag="ACZPCL_08320"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009533779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HIR1162506.1"
                     /translation="MNREEGKNVLILSPLTGRAVPLEEVPDPVFSQKIIGDGMAVIPT
                     EGKILSPADAQIVSVADTGHAYGLKTEEGVELLIHVGLETVSLKGECFKIHVKPGDTV
                     KAGDLLAEVDLEFLEERKLNPITPVLICGGMEGRVLVKSQPGQVEAGKSVLLTETTKE
                     NLKDEQKTQTDPSDNLENSAPEPKSEKKKLSINFDFLQKLGKVLMAVIAVMPAAGLMI
                     SLGKLVQMAGADMSMIFTVGSTMESIGWAIINNLHILFAVAIGGSWAKERAGGAFASV
                     IAFILINVITGALFGISSDMLADPNAVTHTLFGKEILVNGYFTSVLGAPALNMGVFVG
                     IISGFVGGTVYNKYYNYRKLPDALAFFNGKRFVPMVVILWSVIISAVLAVVWPVIQGG
                     INAFGVWIAGSSTTSPVLAPFIYGTLERLLLPFGLHHMLTIPMNYTSFGGTYEIMTGV
                     NAGSKVFGQDPLWLAWANDLINFKSAGDMAAYTNLLTTVTPARFKVGQMIGATGLISG
                     IALAMYKRVDPDKRDKYRSMFLSTVLAVFLTGVTEPLEFMFMFCALPLYLVYAVLQGC
                     AFAMAGIIHLRLHSFGNLEFITRIPMSVKAGLTGDLINFVICVVAFFVIGYVVAYFMI
                     GKFHFATPGRLGNYTDDGAEEEDQGSTQTGSKEKKDSQAERIIALLGGRENIVLVDAC
                     MTRLRVTVKDVDKVAELPAWKAEGALGLLKKDGGIQAVYGPKADVLKSDINDILQ"
     gene            7998..8846
                     /locus_tag="ACZPCL_08325"
     CDS             7998..8846
                     /locus_tag="ACZPCL_08325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015338.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR1162507.1"
                     /translation="MKILTLNTHSLAEENATEKMELFAGILEKEQPEVMAFQEVNQTM
                     AEPFINEKEVCGYQPVKGFEGKMRRDNYGASLAEALRKKGLSYYWTWIPVKVGYDKYD
                     EGLAIFSKSPILSSCVFNLSASDDYYNWKTRKTLGIHTEKGWFFTVHMGWWKDEEEPF
                     KAQWETFLKNTKSLMEKKETSLWVMGDFNSPASVRGEGYDLVRVGGFKDTWDLAEKKD
                     AGFTAPPKIDGWKEAEGKSPEQGMRIDYIWHWGDINIEESRVLCNGKREPAVSDHYAV
                     MIKTGI"
     gene            8895..>10248
                     /gene="malQ"
                     /locus_tag="ACZPCL_08330"
     CDS             8895..>10248
                     /gene="malQ"
                     /locus_tag="ACZPCL_08330"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR1162508.1"
                     /translation="MNRTAGILLSVSSLPSKYGIGCFSKSAYEFADWLKEAGQSYWQI
                     LPLGPTSYGDSPYQSFSTYAGNPYFISLEDLIEEGVLTEKECEKLETESKEGTIDYKK
                     LYEDRYPILRKAYERSNISENPDYQQFLAENEWWLSDYALFMAVKDRFEGRPWTEWAE
                     DIRLRYGYAMDYYRRELYFDIEFQKYLQFTFYKQWNKLKAYVNSLGIRMIGDIPIYVA
                     MDSADAWANPSLFQLDEKNVPYAVAGCPPDGFSATGQLWGNPLYRWEYHRQTGYEWWL
                     KRLSHCFKLYDAVRIDHFRGFDQYYSIPYGAEDATRGHWEQGPGIDLFNRVRQVLGEK
                     EVIAEDLGYVTDSVRKLVYDSGFPGMKVLEFAFDSRDSGCANDYLPHNYPENSVAYTG
                     THDNETLAGWFDSITKEEQNMVRDYLCDHYTPKKYLYKSLNSLVMRSKAALCVIPMQD
                     Y"
BASE COUNT         3120 a         1993 c         2589 g         2546 t
ORIGIN      
        1 cgcttgtgga tataacatct gcaggcgttt tttgttgtta tgtatcagga agaaactgcc
       61 ggtggcaggt tctgcaggtt gctaaaaaac gtctgcacag ataacagaaa gcacattaga
      121 tcagaaagga ggaactgttt tggtaacaat caacggagaa gaaaaaaacg cagctggaaa
      181 aacaatctat gcctatttat cagaagaagg atacgataca agccgtgtag tagtagaaca
      241 gaatttacag atcattcccc aggatcagct tgaaaatacc ctgatccagg aaggtgacca
      301 gatcgaaata ctttgttttg taggaggagg ctgaaaatga cagccataca ggaaaaaatg
      361 caggccctta tttcaaaaga agaactggaa cgggcttttg atgccagatt tccacgggaa
      421 atgaaggaga aattctccaa cgcccgtgtg gcagtagccg gacttggggg actgggatct
      481 aacatagctg taatgcttgc aagaagtggg ataggacatt tgcttttagt ggattttgat
      541 acagtggatg tgacaaattt aaaccgtcag atgtatatga ttcctcattt aggcagaccc
      601 aaggcagaag cattgccgga tatactctgc cagatcaatc catatctgga atatgagtct
      661 gtttgtgtta aagttacacc ggaaaatgta aagcagctat ttgaaatgta tcccattgtc
      721 tgtgaagcct ttgacaaacc agatcagaaa gccatgctgg taagagaact tttaggacaa
      781 tatccggaga caatggtcgt ttcaggaaat ggaatggcag gtctgggaga taccaatgac
      841 atacagacaa ggaagatgat gagacggctt tatgtatgcg gggatcagaa aacagatgta
      901 ggaaacggga tcggccttat agcgcccaga gtagcagcct gcgctgccca tcaggccaat
      961 aaagtactgc agctgatcct gcagtcataa acagaagaag cctgtaaaca gaaaaatata
     1021 taaatatgta catatgtgaa aagagaggaa aacaacatgg aagataaact ggttcttggg
     1081 ggaaaagaat ttagcagccg ttttatttta ggttccggca aatataatat caatctgatc
     1141 aaggcagcgg tagaacaggg cggcgccgag attattactc tggcattaag aagagcaaat
     1201 acccaggaaa aagaaaatat tttggatcat atcccggaag gagtaacatt actgcccaat
     1261 acttccggtg ccagaaacgc agaagaagca gtgcgtattg ccagactgag ccgggctatg
     1321 ggctgtgggg attttgtcaa agtagagatt atgcgtgata caaaatatct gtttccggat
     1381 aattatgaga cagtaaaagc gacggagacc cttgcaaaag agggctttat cgttctgcct
     1441 tatatgaacc cggacttaaa tgtggcaaga gatctgcaaa gcgccggcgc agcagccgtt
     1501 atgcctttag cagctccgat tggaagcaac aagggtctgg caaccaaggc catgatccag
     1561 gtactgatcg acgagatcga cctgcctatc attgtagatg caggtattgg caagccgtcc
     1621 caggcatgtg aggcaatgga aatgggtgct gcggcagtta tggcaaatac tgctattgca
     1681 acggccggag atgtgcctgc tatggcaaat gcctttaagc tggccattga agcaggaaga
     1741 aaagcatacc ttacaggaat gggaagagtc ctggcaagag gcggcagcgc atctgatccg
     1801 cttacaggct ttttacgtta ggaggcagcg gacatgaacg aagaaaacca ggtctatttt
     1861 gtagacagtg ataagctgcc tgaggaagca ttaaagcgga agcaccgctt agagcaggat
     1921 ccaactttcc gtaccaacca catggaatac atggaaggga tggaagtgag ccggtctgac
     1981 atcaaggaca aagtggaaga ggccatgaaa gcctttgatt atgatgccta tacggcaggg
     2041 gatgtagagc gggcgctgtc ccatgaaaca tgttccattg aagattttaa ggcactttta
     2101 tcccctgcag cggctcctta tctggaggta atggccagaa aagctgagga gatcacaaaa
     2161 aatcattttg gcaatacggt atacattttt acccctcttt atattgccaa ttactgccag
     2221 aattactgca tttactgcgg ttttaactgc tacaacaaca tccgccgcaa aaaacttaca
     2281 tttgaagaga tcgagcatga aatgcaggtg atcgcaaaga ccggtatgga agagatcctg
     2341 atccttacag gggaaagcag ggcctattcg gatgtaaaat atatcggaga agcagtgaag
     2401 attgcccgga aatatttccg aaatatcggc attgaaatct atcctgtaaa tgtagatgag
     2461 tataaatatc ttcatgaatg tggcgtagat tatgtaacgg tattccagga aacctacaat
     2521 acagataagt atgaaaccct gcatcttatg ggacataaac gtgtatggcc ataccgcttt
     2581 gatgcccagg agcgggctct tatgggaggt atgagaggtg caggcttttc cgcgctgtta
     2641 ggtctggatg atttccgaaa ggatgctctt gcaacggctc ttcatgtata ttatatcaat
     2701 cggaaatatc cacatgcaga gctttcttta agctgtccaa gactacgtcc tatcgtcaat
     2761 aatgacaaga tcaatccaag ggatgtaggg gaaaaagaac tttgccaggt tctctgtgca
     2821 taccgtattt tcctgccatt tgcaggtatt actgtatcca gccgtgaaag cgctgctttc
     2881 agaaacggca ttaccaagat ttgtgcaaca aaggtttctg ccggtgtatc cacgggaatt
     2941 ggagatcatg aggagaaata cgaaggcaag gaagatgcag acgtaggaga tgagcagttt
     3001 gagattaatg acggacgttc tttcgccagt atgtatgagg atatggaaaa aggcggtctg
     3061 cagccggtcc ttaacgacta catttatgta taatagaaga acttgataga caaaaaacac
     3121 acagcttgtc atctttaggc tgcttttgtg gggaaacatc agagattgta aaaagtaaga
     3181 ctgaaatcgt aatagaaaag taataaaagg atgtgctata ctaaaactaa gtaaaaaacg
     3241 tctgaaaaca gatgtctggg gtaggacgat aggttcggga aatctgcagg gacagacgga
     3301 acagaaggag aagttttatg aagaagacgg gaaaaatatg ggtagcactt attacagcac
     3361 tcagtcttgc catgccaatg aatgtgtggg cagacacgaa gatcaccagc atctcattga
     3421 agatcgattc gtccattgaa gcaggagaca gcgactgtga cgtggatgta actacttcct
     3481 caagatactg cagtgtagat gatgtggaag ttacaaacga gccgtccgac gagtggaaga
     3541 gcggtgcaag accaaagatc aaggttacat tatcctcaga tggtgatgcg tactttggca
     3601 caggtattac aaaaagtgat atttctgtca gtggaaatga tgctaccgtt acatcagtca
     3661 gtcgttccgg caagtatgaa ctgaatgtca gtcttactct ggaaaaactg gaacgtgact
     3721 ccagtgatta tgatctggat gtaacagggt taaactggga tgattatgac ggaactgcaa
     3781 gctgggaaga gccggaagat gcaaagcgct atgaagtacg tctttacaga gacggaagca
     3841 gcgtttcttc tgcaatctct actacagaca ccagctacaa tttcgccagc tatttcacaa
     3901 gaagcggtga ttacacattt aaggtacgtg cgatctacaa cacctccaac aaaggtgact
     3961 gggaagagtc agacgaatta agcgtttcct ccagcgaagc aaaagatatc cgtgacaatg
     4021 gaaaatcctc cggaaataca ccttccaatt ccatagatga tgacggcggc cctggagaca
     4081 caaacactag tcagggcgca tggcttaaaa atgacaccgg ctggtggtac tgcaacgcgg
     4141 accgcagcta tccggtaaac cagtggcagt atatcaacaa ttactggtac ttctttaatg
     4201 catccggcta tatggtaact ggctgggtac agtggaataa cgtatggtat tactgctgtg
     4261 acagtggaga gatgcttacc aatacccgta caccggatgg ctattatgta gatggaaacg
     4321 gggcatggat ccagtagaaa acttaaaacg gtcaaatgag gacattctaa tttttcggcg
     4381 ggcagcaact atccctctct accatatgat gaggcaccag gatcttggtg gcaggaagcc
     4441 cgggatcttc aatgtggttg atcatctggg tagcggcttc tgcgccaagc tggaaagaat
     4501 tgatatccac agaagtaagc tgaggcgaag caagccttgc aaacagtgag ttattaaaag
     4561 agacaatgga aagatcttcc ggtatggaaa gacccatttc caggcagaca cgttctaagg
     4621 ttgcagccag gatatcatca ctgactacaa cagcagtagg acggtccggt aaagagagaa
     4681 gacagcgtaa aggtgcgctg tctttttctg ttgctgtaga tgacgcactg gtgccgcctg
     4741 cgtttatggc agcagtcatt tccacgcagt aatccactct tggaaggata ttgtgctgta
     4801 aaagggcagt ctgatagccg cttttacggt ctgcggagaa gatcatggaa gtatcacttc
     4861 caatgtagcc gatccgtctg tgtcccatct ggtacagata ctccgttgct tcctgagcag
     4921 ccaggagatt atcattatca atgtagatcg tctggtttgc atgggaatag gcttttccta
     4981 ccagcacata aagaaatcct tcattataga gaaaatcagc aatggggtca tcctggcggg
     5041 aataaagaac gataaaaccg tctacctgtc cgctgcgcgc catggtcttg atcacctgaa
     5101 ggacttcttc ttcactttca cctgtgatca cggtgttgat atactgccgc ttgttgcaca
     5161 gctggctgat cccgcggatg gcttccagat aaaaggagtt ttcgtatgcc tctctggcgg
     5221 aaacaggcag aatgatcccg atggtacggg aattctggga agccagattg ctggcctgga
     5281 agttgggctc atatcctaac tgcgccatgg cccggcgaac tttttctttt gtttcctcac
     5341 tgatagaggg attgttttta caggttctgg atacagtaga agaagataca cctgccagag
     5401 ctgctacatc ttttatggta accgccatga taaaacctcc tgggatcatt aaaaatcgtc
     5461 tgatattatt ttaaaagaga ataagagcct tgtcaataaa gattgcgcaa actcagtgca
     5521 aaaattgcat aaaatattgc aggaaagaag agacacctct ggagttgata aaacaactgg
     5581 aaaattgcac aaaacagggc agaataaatt gtgcaaaaaa tagaaaacct ggaaaatata
     5641 gaaaatacac ttgaaaaagc aatgcaaact tattatattt aatgcaagcg gttgcgcaat
     5701 cttgcaaaaa acatgagacg gggcacaaaa ggaaagcagg ccgcaaaaga atgtccggga
     5761 gggagcaatt tatgaacaga gaagaaggta agaacgtgtt gatactgtca ccactgacag
     5821 gcagggcagt accgttggaa gaggttccgg atcctgtgtt ttcacagaag atcataggag
     5881 atggaatggc ggtcattcca acagagggga agatcctaag cccggcagac gcacagatcg
     5941 tatccgtagc agacacaggt catgcctatg gtctgaaaac agaagagggt gtggaacttc
     6001 tgatccacgt tggtcttgaa acagtatcat taaaagggga gtgttttaag atacatgtaa
     6061 agccaggtga cactgtaaaa gcgggagatc tgttagcaga ggtggatctt gaatttctgg
     6121 aagaacggaa gctcaacccc atcactcctg tgctgatctg cggcggaatg gaaggaagag
     6181 tcctggtaaa gagtcagcca ggacaggtgg aagccggaaa gagcgtgctt ttgacagaaa
     6241 caaccaaaga gaacctgaaa gatgaacaaa aaacacagac agatccatcc gataacctgg
     6301 aaaattcagc accagagcca aaatcagaaa agaaaaagct gtccattaac tttgactttt
     6361 tacagaaact gggaaaagta ctgatggcag taattgcagt tatgcctgcg gcaggtctga
     6421 tgatcagcct tggaaaactg gtacagatgg caggagccga tatgagcatg atcttcacag
     6481 tggggagcac tatggagagc attggctggg ccatcatcaa taacctgcat atcctttttg
     6541 cagtagctat tggcggttcc tgggcaaaag aacgggccgg cggtgccttt gcatctgtga
     6601 tcgcctttat cctgatcaat gtaattacag gagcgctgtt tggcatctct tctgacatgc
     6661 tggcagatcc aaacgcagtt acccatacac tgtttggaaa agagatcctt gtaaatggat
     6721 actttacctc tgttttaggc gcgcctgcat tgaatatggg tgtgttcgta ggtatcatat
     6781 ccggtttcgt aggaggtact gtttacaata agtattacaa ttacagaaaa cttcctgatg
     6841 cactggcatt tttcaatgga aaacgttttg tacctatggt agtgatcctg tggtctgtga
     6901 tcatttccgc tgttttagca gttgtatggc cggtgatcca gggcggtatc aatgcatttg
     6961 gcgtgtggat cgcaggttct tccaccactt caccggtact ggcaccattt atttacggta
     7021 ccttagagcg tctgctcctg ccgtttggcc ttcatcatat gttgaccatt cctatgaatt
     7081 atacttcttt tggcggtact tatgagatca tgactggcgt aaatgcagga agcaaggtat
     7141 ttggacagga tcctttatgg cttgcatggg ccaatgacct gatcaacttt aaaagcgcag
     7201 gagacatggc ggcatataca aaccttctta caaccgttac accggcccgc tttaaggtag
     7261 gacagatgat cggtgctacc ggactgatct ccggtattgc tcttgcaatg tataagagag
     7321 tagatccgga taaaagggac aaatacagat ccatgttttt atccacagta cttgctgtat
     7381 ttttaacagg cgtaacagag ccactggaat ttatgtttat gttctgtgcc ctgccgttat
     7441 atctggttta tgcagtactt cagggatgcg catttgcaat ggccggtatt atccacctgc
     7501 gtcttcattc ctttggtaac ctggaattta tcacccgtat ccccatgtct gtaaaagcag
     7561 gactgaccgg ggatctgatc aattttgtaa tctgcgtggt agcattcttt gtgatcggat
     7621 atgtggttgc ctattttatg atcggaaaat tccattttgc aacaccggga cgtctgggaa
     7681 attatacaga tgacggagca gaagaggaag atcagggcag tacacaaact ggaagcaaag
     7741 aaaagaagga ctcccaggca gagcgcatca tcgctctttt aggcggcagg gaaaatattg
     7801 ttttagttga tgcctgcatg acaagacttc gtgtgactgt aaaagatgta gataaagtag
     7861 cagaactgcc tgcatggaag gcagagggag cattagggct tttaaagaag gatggcggta
     7921 tccaggccgt ttatggacca aaggcagatg tgctgaaatc cgatattaat gatattttac
     7981 aatagaaagg ctgtaaaatg aagatcctta cgttaaatac acacagtttg gcagaagaaa
     8041 atgccacaga aaaaatggaa ctgtttgccg gtatcctgga aaaagagcag ccggaggtca
     8101 tggcatttca ggaagtaaac cagaccatgg cagaaccatt tataaatgag aaagaagttt
     8161 gtggttatca gccggtcaaa ggctttgaag ggaaaatgcg ccgggataat tatggtgcgt
     8221 ctctggcaga agcgttaagg aaaaaaggtc tttcctacta ctggacctgg atccctgtaa
     8281 aagtaggata tgataaatat gacgaaggac tggcaatctt ttccaaaagt cccattcttt
     8341 cttcctgtgt atttaactta agtgcttctg atgattacta taactggaaa accagaaaaa
     8401 ctctgggaat acacacagaa aagggctggt tttttacggt gcatatgggc tggtggaagg
     8461 atgaagaaga gccttttaaa gcccagtggg aaacattttt aaagaataca aagagcctga
     8521 tggagaaaaa agaaacatcc ctgtgggtca tgggagattt taacagtcct gcttctgtaa
     8581 ggggagaagg ctatgatctt gtaagagtag gcggttttaa ggatacatgg gatctggcag
     8641 agaagaaaga tgccggattt acagcaccgc caaagatcga tggctggaag gaagcagaag
     8701 gaaaatcgcc tgagcaggga atgcgcatcg actacatctg gcattgggga gatattaata
     8761 tagaagagtc ccgtgtttta tgcaacggga aaagagaacc ggcagtttcg gatcactatg
     8821 cggtgatgat caaaacaggt atatagaacc gttgcaacag gcattaaaaa cagggaaaga
     8881 ggagcgtaaa taaagtgaac agaacagcag gaatactttt atctgtatcc agtctgccat
     8941 caaaatacgg cattggctgc ttttcaaaaa gcgcctatga atttgcagac tggttaaaag
     9001 aagcaggtca gagctactgg cagatccttc ctttaggacc taccagctac ggcgactctc
     9061 cgtaccagtc attttccacc tatgcaggaa atccttattt tatcagctta gaagacctga
     9121 ttgaagaagg tgttcttaca gaaaaggaat gtgaaaaact ggaaacagag tccaaagaag
     9181 ggaccatcga ctataaaaag ctgtatgagg accggtatcc tattttgcga aaggcctatg
     9241 agcgcagcaa tatcagtgaa aatccggatt atcagcagtt tcttgcagaa aatgaatggt
     9301 ggctttctga ttatgccctt tttatggcag taaaagaccg gtttgaggga agaccatgga
     9361 cggagtgggc agaagatatc cgcctgcgtt atggctatgc catggattat tacagaagag
     9421 aactgtattt tgacatagaa tttcagaaat acctgcagtt taccttttat aaacagtgga
     9481 ataaattaaa ggcctatgtt aacagtctgg ggatccgcat gatcggggat attcccattt
     9541 atgtagctat ggacagcgca gatgcctggg cgaatccttc tttgttccag ttagatgaga
     9601 aaaatgtgcc ttatgcagta gccggctgcc cgccggacgg attttctgct acaggccagc
     9661 tttggggcaa tcctttgtac cgctgggaat atcacagaca gaccgggtat gaatggtggt
     9721 taaagcggct ttcccactgt tttaaactgt atgatgcagt gcgcattgac catttcagag
     9781 gatttgacca gtattattct atcccttatg gagcggaaga tgctaccaga ggccactggg
     9841 aacagggacc ggggatcgat ctttttaacc gggtacgtca ggtgctggga gaaaaggaag
     9901 tcattgcgga agatcttggc tatgtaacag actctgtgcg caaactggtt tatgacagcg
     9961 gttttccagg tatgaaggta ctggaatttg cctttgactc cagagattcc ggttgtgcca
    10021 acgattacct gcctcacaat tatccggaaa attccgtagc ttatacagga acccatgata
    10081 atgaaactct ggcaggctgg tttgacagca ttacaaaaga agaacagaat atggtacgtg
    10141 attatctgtg tgatcattac actccaaaga aatatttata taagtcttta aacagcctgg
    10201 taatgcgttc taaagctgcc ctttgtgtga tcccaatgca ggactatc
//
LOCUS       DBMDUA010000097        10234 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3186_length_10234_cov_2.762657, whole genome shotgun sequence.
ACCESSION   DBMDUA010000097 DBMDUA010000000
VERSION     DBMDUA010000097.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10234)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10234)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10234
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3186_length_10234_cov_2.762657"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(197..1573)
                     /gene="mtaB"
                     /locus_tag="ACZPCL_08335"
     CDS             complement(197..1573)
                     /gene="mtaB"
                     /locus_tag="ACZPCL_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035598 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIR1162509.1"
                     /translation="MPKAALHNLGCKVNAYETEAMEQQLKERGYEIVPFDEKADVYII
                     NTCSVTNIADRKSRQMLHRAKKLNPESVVVAAGCYVQVASEELKKDASVDIVIGNNKK
                     AELADILDEYLGNVHEEEEAEVFVTDLSHGSDYEALHLEQPSDHTRAFIKVQDGCNQF
                     CSYCIIPYARGRVRSRSREDVVKEITGLVAQGYKEAVLTGIHLSSYGIEHMEGNPVSQ
                     GDWNHRELLALIREVHEIEGLERIRLGSLEPRIITEEFAKSLAELPKFCPHFHLSLQS
                     GCDSVLKRMNRHYTTEDYLRRCEILRKYFKRPAITTDVIVGFPGETEEAFDITEKYLE
                     KVHFYEMHVFKYSRRAGTKAAAMPDQIPEQIKGVRSDRLLALEQKMSEEFRKGFIGEK
                     VSVLFEEMITVDGKEYLVGHTPEYVKAALPKEECEKMHAASGVILEAEAKDLLTSDIL
                     LLKNTIAE"
     gene            1744..1977
                     /locus_tag="ACZPCL_08340"
     CDS             1744..1977
                     /locus_tag="ACZPCL_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1162510.1"
                     /translation="MKTVRISLNSIDKVKSFVNDLAKFDVDFDLVSGRYVIDAKSIMG
                     IFSLDLSKPIDLNIHAENDVDSILSTLSPYIID"
     gene            complement(2241..3431)
                     /gene="thiI"
                     /locus_tag="ACZPCL_08345"
     CDS             complement(2241..3431)
                     /gene="thiI"
                     /locus_tag="ACZPCL_08345"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HIR1162511.1"
                     /translation="MQYQSFLIKYAEIGTKGKNRYMFEDALIRQIRYALSSVDGEFDV
                     TKESGRIYVKALGEYDYDDTIEALKRVFGIADICPMVQIDDKDYENLKKHVVEYMDQV
                     YPDKNITFKVDARRGDKQYPVSSEQINRDMGEAILDAFPEMKVDVHHPDVLLRVEIRQ
                     KVNLFSLMIPGPGGMPIGTNGRAMLLLSGGIDSPVAGYMIAKRGVKIDAVYFHAPPYT
                     SERAKQKVVDLANLVARYSGPINLHVVNFTDIQLYIYEQCPHEELTIIMRRYMMRIAQ
                     AIAEKTGSIALITGESIGQVASQTLQSLASTNEVCTMPVFRPVIGFDKQEIVDVSEKI
                     GTYETSIQPYEDCCTIFVAKHPVTKPNLKVIRNSERHLEEKIDEMVKTALDTVEVIAC
                     RGEV"
     gene            complement(3497..4651)
                     /locus_tag="ACZPCL_08350"
     CDS             complement(3497..4651)
                     /locus_tag="ACZPCL_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR1162512.1"
                     /translation="MEAYFDNSATTKALDSVRDIMVKTLTEDFGNPSAKHTKGMEAEN
                     YIRQAAADIAKTLKVKDKEILFTSGGTESNNMALIGTALANQRAGKHIISTRIEHASV
                     YQPLAFLESLGFEVTYLHVDHNGHISLEELEQSIRPDTILVSVMYVNNEIGAIEPIDE
                     IARLVHGKNPKIVFHVDAIQAYGKLVIRPKKQGIDLLSVSGHKIHGPKGIGFLYIDEK
                     VKIRPLIYGGGQQKDMRSGTENVPGIAGLGVAAKEMYTDHSAKMEYLTGLKDYLIDKA
                     SELEGVTVNSLKGAEGAPQIVSLSFEGVRSEVLLHALEEKGIYVSSGSACSSNHPAIS
                     GTLKAIGVKKELLDSTLRFSFGMFNKKEEIDYAVDVLKELLPVLRRFTIK"
     gene            complement(4651..5400)
                     /locus_tag="ACZPCL_08355"
     CDS             complement(4651..5400)
                     /locus_tag="ACZPCL_08355"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR1162513.1"
                     /translation="MHHFFVDPSSIEEGIVRIVGTDLNHMKNVLRMKPGEEVLISDGT
                     GKDYNCQVKEYGAEEGILVILSENADSRELPSRIWLFQGLPKSDKMEVILQKAVELGA
                     AGVIPVATRNAVVKLDAKKAEAKIRRWQAIAESAAKQSKRSYIPQVGPVMSLKEAFSY
                     IEEQKFDLRMIPYELEKGMDGTKTVLEALAPGQQVAVFIGPEGGFDEEEIQLALKMGV
                     KPVSLGKRILRTETAGPAILALLMMKLEGAF"
     gene            complement(5410..6366)
                     /gene="prmA"
                     /locus_tag="ACZPCL_08360"
     CDS             complement(5410..6366)
                     /gene="prmA"
                     /locus_tag="ACZPCL_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR1162514.1"
                     /translation="MKWKKFTLTTTTEAVDLVSSMLDDVGIEGIEVEDNVPLTEKETK
                     GMFIDILPELPPDEGVAKVSFYLDDDDQVEEMLRRVNEGLDELAAYTELGQRTIEASE
                     TEDKDWINNWKQYFKPFTVDHILIKPTWETVPEEHKDKLLVQIDPGTAFGTGMHETTQ
                     LCIRQLEKYVHSESEILDVGTGSGILGITALKLGAKEVWGTDLDENAITAVGENLASN
                     DISSDRFHVLQGNIIDDKSVQDWAGYEKYDIVVANILADVIIMLVKEIPVHLKKGGYF
                     ITSGIINLKEEAVRAAFAASEELEVVEITYQGEWLSVTARKK"
     gene            complement(6382..7137)
                     /locus_tag="ACZPCL_08365"
     CDS             complement(6382..7137)
                     /locus_tag="ACZPCL_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR1162515.1"
                     /translation="MTAYLIVCPLVFLAGLVDSIAGGGGLISLPGYLIAGVPAHLALG
                     TNKMSSTIGTSISAVRLLKHGYLKGKMKMAIASAVCAMTGSVLGARMSLLVSESVIRH
                     MMIVVLPIVAYHVLKNKHMGEDEKTGTASYKKMFLISVIAAFFIGCYDGFYGPGTGTF
                     LLLVFTGAAKMDTRSASAQTKVINLSSNIAALVTFIIAGNVYYPLGLAAAACSVAGNY
                     LGSGLVVHDGQKIVRPVVLVVLGILFIKILTGN"
     gene            complement(7538..8647)
                     /gene="rsmH"
                     /locus_tag="ACZPCL_08370"
     CDS             complement(7538..8647)
                     /gene="rsmH"
                     /locus_tag="ACZPCL_08370"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR1162516.1"
                     /translation="MEEKRINSVGETADTIGGGENLETPHKRRVRYKGTHPRSYKEKY
                     KELQPEKYGDTIAKVISKGSTPAGMHISIMVKEILDFLNIQPGQTGLDATLGYGGHTS
                     HMLACLKGEGHMYALDVDTIEMEKTRKRLADKGFGPDILTIKHLNFANIDQVAEEAGG
                     FDFVLADLGVSSMQIDNPDRGFSFKNEGPLDLRLDPSKGETAAERLKKLNFEELKGML
                     IENSDEPYAEQIARKVMNEKKHGRPIETTTRLKEVIEEALSFLPEAEKKEAVKKSCQR
                     TFQALRIDINSEFEVLYAFLDKLPGALKPGGRAAILTFHSGEDRLVKKAFKEGLKAGI
                     YSEINQDVIRPSAEECAKNGRAKSTKLRWAVKAQE"
     gene            complement(8730..>10234)
                     /locus_tag="ACZPCL_08375"
     CDS             complement(8730..>10234)
                     /locus_tag="ACZPCL_08375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HIR1162517.1"
                     /translation="EDFLRFALDPQVNISMVAVDEAHCVSQWGQDFRPSYLKIVDFIK
                     RLPKRPVVSAFTATATAEVRDDIIDILMLQEPEVLTTGFDRTNLYFGVQTPKDRYQAL
                     VELLEQHKSESGIVYCLTRKIVEEVCEKLIKEGFSVTRYHAGLSDAERKHNQEEFIYD
                     NVRIMVATNAFGMGIDKSNVRFVIHYNMPKNMESYYQEAGRAGRDGEPAECILLYGGQ
                     DVITNQFFIDHNQDNEALDPMTRQLVMKRDRERLRKMTFYCFTHECLRDYILRYFGEY
                     GSNYCGNCSNCLTQFENTDITEMAKALLSCIETSRQRYGATVIIDTVHGANTAKIRGY
                     GMNENLEYGSLAKVPVYRLRQVLNQLQLEGYLTATNDEYAILRLTPKAASVLNNEETV
                     WMKLAKEQTKSEQETQKKSRKKKSALAGSGEFTEAEENLFETLRKLRAQIAKEEKVPP
                     YIVFSDKTLAHMCIIKPVNKEEMLSVSGVGEFKYEKYGERFLAAVREQTE"
BASE COUNT         2616 a         2635 c         1888 g         3095 t
ORIGIN      
        1 tccgtagctt gaataatctt accggtggcc atttcaataa aataaatttg tgtaggagtc
       61 catttacgtt tttctccgtt aggaagcgta ctttctacct ttttgtcctg aacagcctga
      121 aaatactgtg tttcttttat attgcccata tcatcacgtc ctttacgtat tcttatatat
      181 tctactctat atttttctat tcggcaatag tattttttaa cagaagtata tcggaagtca
      241 gaagatcttt tgcttctgcc tctaaaataa caccggaagc agcatgcatc ttctcacatt
      301 cttccttagg aagagctgct ttcacatact ctggcgtatg tcccaccaga tactcttttc
      361 catctactgt tatcatttcc tcaaaaagaa cagaaacctt ctcaccaata aatccttttc
      421 tgaactcctc tgacatcttc tgttccaaag ctaataagcg gtcagaacgg actcccttga
      481 tctgttccgg gatctgatcc ggcatggctg ctgcctttgt accggctctt ctggaatatt
      541 taaacacatg catctcataa aaatgcactt tttccagata cttttccgta atatcaaacg
      601 cttcctctgt ctctcccgga aagcctacga tcacatccgt agtaatagcc gggcgcttaa
      661 aatatttacg caggatctca caccgtctta aataatcttc tgtggtataa tgacggttca
      721 tccgttttaa aacgctgtca cagccgctct gtaaagacag gtggaaatga ggacagaact
      781 taggaagctc tgccagactt ttagcaaact cttctgtaat aatacgcggc tccagtgatc
      841 ccaggcggat gcgctccagg ccttcaatct catggacttc acggatcaga gccagtaact
      901 ccctgtggtt ccagtcaccc tggcttacag gatttccttc catatgctca attccataag
      961 agcttaaatg aattcctgta agaaccgctt ctttatatcc ctgtgccacc agtcctgtga
     1021 tctctttcac tacatcttca cggcttctgc tgcgcaccct gcctctggca taagggatga
     1081 tgcaatagct gcagaactga ttacagccat cctggacctt gataaaagct cttgtatggt
     1141 cagaaggctg ctctaaatga agggcctcat aatcactgcc atgggaaagg tccgttacaa
     1201 atacctctgc ctcctcttcc tcatggacat tgcctaaata ttcatccaga atatctgcaa
     1261 gctctgcctt cttattgttt ccaattacaa tatccacgct ggcatctttc ttcaactctt
     1321 ccgatgccac ctgcacataa cagcctgctg ccaccactac agattctgga ttcagcttct
     1381 ttgcacgatg gagcatctgt cttgatttgc ggtccgcaat attggtaaca gaacatgtat
     1441 tgataatata aacatccgcc ttctcatcaa aaggaacgat ctcataacct ctttccttta
     1501 actgctgctc catggcttct gtttcataag catttacttt gcaccccaga ttgtgaaggg
     1561 ctgcttttgg cataatataa cctcctgatt tttccaaata tttacaatta taacttttcc
     1621 cgtttcttcc tgatttttca tacaatctca ctattgactt ttataccact gacctttagt
     1681 attaaaagta agcagtttta ttctgcaaaa atgatttaca taccacacaa ggaggggctt
     1741 cctatgaaaa cagttagaat ttcattaaat tccattgata aggttaaatc tttcgtaaat
     1801 gatttagcta agttcgatgt tgattttgat ctggtttccg gcagatatgt catcgacgca
     1861 aaatctatta tgggtatctt cagcctggat ctgtccaagc cgatcgatct gaacatccat
     1921 gcagaaaatg acgtagacag cattctttct acactttctc catacatcat cgactgagtt
     1981 taatgttaaa gttagaaggg agccgcggga atgcggctct ctttttttat acatcacttc
     2041 ccgccctaca ggggcgggga cttttagcca gaaataacgt tgcaggggcc gctttcaata
     2101 aagcgattgt acgcatcccg agcgcagccg tacgtccgta acggacgcag tccggcacag
     2161 gcgaactgac gcaagcgaag gccagcgaac aatgttttat agaatgaaga accccctcgc
     2221 aaatacttca gccagcaacc ttaaacctcg ccgcggcaag caataacttc cactgtatct
     2281 aacgcagtct tcaccatctc atcgatcttc tcttccagat gacgctcaga atttctgatc
     2341 accttaagat ttggcttggt aaccgggtgc tttgctacga aaatcgtgca gcagtcctca
     2401 tatggctgga tggaagtctc ataagtaccg atcttctcag aaacatccac gatctcctgc
     2461 ttgtcaaatc caatcaccgg acggaaaaca ggcatggtgc acacttcatt ggtagaagcc
     2521 agtgactgta acgtctggga agccacctgt ccgatactct cgcctgtgat cagggcaatg
     2581 gaaccggtct tttcagcgat cgcctgtgca atacgcatca tataccggcg cataatgata
     2641 gtcagctcct catgagggca ctgctcataa atataaagct ggatatctgt aaaattaact
     2701 acatgaagat tgatcgggcc ggaataacgt gctaccagat tagccaggtc aactaccttc
     2761 tgctttgctc tctcactggt gtatggcggc gcatggaaat aaacagcgtc gatcttaaca
     2821 cctcgctttg cgatcatata acctgcaacc gggctgtcaa taccaccgga aagcagcagc
     2881 attgcccgac cattggtacc gataggcata ccacccggtc ccgggatcat cagggagaac
     2941 aggtttacct tctgacggat ctccacacga aggagaacat ccggatgatg tacatctacc
     3001 ttcatctctg ggaaagcatc taagattgcc tcacccatat cacggttgat ctgctcagaa
     3061 cttacaggat actgcttatc tcctctcctg gcatctacct taaatgtgat attcttgtcc
     3121 ggatatacct gatccatgta ttctactaca tgctttttca ggttctcata atccttatcg
     3181 tcgatctgca ccattgggca gatatctgcg ataccaaata cacgctttaa cgcttcaatg
     3241 gtatcatcat aatcatactc tcctaaagcc tttacataaa tacggccgga ttcctttgta
     3301 acatcaaact caccgtctac agagcttaag gcataacgga tctgacggat cagggcatct
     3361 tcaaacatgt atctgttttt gccctttgtg ccaatctctg catacttgat caaaaatgac
     3421 tggtactgca taatacttcc tttcttttgt tctttatact tgacgtactc cactgcctgc
     3481 tgacgcaggc ggtcgactat ttgatagtaa atctcctaag caccggcaga agctccttca
     3541 aaacatccac tgcatagtcg atctcttctt ttttattaaa cattccaaaa ctaaatctca
     3601 aagtggaatc caataattcc ttcttcactc caattgcctt taaagtaccg ctgatggccg
     3661 ggtgattaga agaacatgct gaaccggaag aaacatagat acctttttct tccagtgcat
     3721 gaagaagcac ttcacttctc actccctcaa aacttaaact tacgatctga ggagcgcctt
     3781 ctgcaccctt taagctgttg actgtaacac cttccagttc agaggcctta tcgatcagat
     3841 aatccttcaa accagtaaga tactccatct tggcactgtg gtctgtatac atttctttgg
     3901 ctgccacacc aagacctgca ataccgggaa cattttctgt accggaacgc atatccttct
     3961 gctggccacc gccgtagatc agcggacgga tctttacctt ttcatctata tataagaaac
     4021 caatgccttt tggaccatgg atcttatgtc cgctgacgct taacagatca ataccctgct
     4081 ttttcgggcg gatcaccagt tttccatatg cctggatagc atctacgtga aatacgatct
     4141 tcgggttctt tccatgtacc agacgggcga tctcatcaat tggctcaata gcaccgatct
     4201 cattatttac atacataaca gacactaaaa tagtatccgg gcggatagac tgctccagtt
     4261 cttccagtga gatatgtccg ttgtggtcca catgaagata agtcacctca aagcctaagg
     4321 actccagaaa tgccagtggc tgataaacgg atgcatgctc gatcctggta ctgataatgt
     4381 gctttcccgc tctctgattt gccagcgctg taccaatcaa agccatgtta ttggactccg
     4441 taccgccgga agtaaacagg atctccttat cttttacctt taaggtcttt gcaatatcgg
     4501 ctgcagcctg acggatgtag ttttctgctt ccataccctt tgtatgcttg gcagagggat
     4561 ttccgaaatc ctccgtcagg gtttttacca taatatcccg gacagagtcc agtgcctttg
     4621 ttgtggctga attgtcaaaa tatgcttcca ttaaaatgct ccttccagct tcatcatcag
     4681 aagagccagt atggcaggtc ctgctgtttc cgtgcgcagg atacgctttc caagactgac
     4741 aggttttact cccattttca gcgcaagttg gatctcttcc tcatcgaaac cgccttccgg
     4801 tccgataaac actgcaacct gctgtcccgg cgccaaagct tctaatactg tcttcgtccc
     4861 gtccattccc ttttccagtt cataagggat catgcgcaga tcaaatttct gctcttctat
     4921 ataggaaaat gcttctttta agctcatcac aggtcccacc tgagggatat aactgcgttt
     4981 ggactgtttg gctgcacttt ctgcgattgc ctgccagcgg cggatcttcg cctctgcctt
     5041 ctttgcatcc agcttcacta ctgcatttct ggttgcaacc ggtatcacac ctgcagctcc
     5101 cagttctact gctttctgca ggatcacttc cattttatct gattttggca atccctgaaa
     5161 gagccagatt ctggaaggaa gttccctgct gtctgcattt tccgaaagga tcactaaaat
     5221 tccttcttct gctccatact cttttacctg gcagttataa tcttttcctg tgccatcgct
     5281 gatcagcact tcttctcccg gtttcatgcg gagcacattt ttcatatggt ttaagtccgt
     5341 tccaacgatc cttacaatcc cttcctcaat actggaagga tctacaaaaa aatgatgcat
     5401 cccgaactcc tatttctttc ttgcagtaac agagagccat tctccctgat aagtgatctc
     5461 tacaacttcc agttcttcac tggcagcaaa tgcagcccgg actgcttctt ccttcaggtt
     5521 gataataccg gaagtaatga aatatccacc ttttttcaga tgtaccggga tctcttttac
     5581 cagcatgatg ataacatctg ctaaaatatt ggcaactaca atatcatatt tctcatatcc
     5641 ggcccagtcc tgaacagact tgtcatcaat gatatttccc tgaagtacat ggaaacggtc
     5701 actgctgata tcattggaag ccaggttctc accaactgct gtaatggcat tttcatccag
     5761 atccgttccc cagacttctt ttgcacccag ctttaaagct gtgataccta aaataccgct
     5821 tcctgttcct acgtccaaaa tctcactttc actgtgtaca tatttttcca gctggcggat
     5881 gcaaagctgg gtagtctcat gcataccggt accaaaagca gttcccgggt cgatctgtac
     5941 taacagttta tccttgtgtt cttccgggac agtctcccag gttggcttga tcaggatatg
     6001 atctactgta aacggcttaa aatactgctt ccagttgttg atccagtcct tatcctctgt
     6061 ctcagatgcc tcaatagtgc gctgtcccag ttctgtataa gcagcaagct catccagtcc
     6121 ttcattgaca cggcgcaaca tttcctctac ctgatcatca tcatccagat aaaagcttac
     6181 ctttgcaacg ccttcatccg gcggaagctc cggaaggata tcaataaaca ttcccttagt
     6241 ttctttttca gtaagaggaa cattatcttc tacttcaatg ccctcaatac ccacatcatc
     6301 cagcatgctg cttacaaggt caactgcttc tgtggtagta gtcagagtga attttttcca
     6361 cttcataatg catttctcct tttaattccc cgtcagaatt tttataaata agatccccag
     6421 taccaccaga acaaccggac ggacgatctt ctggccgtca tgaactacca gccctgaacc
     6481 caggtaattg cctgctacag aacaggctgc tgctgccagc cccagaggat aataaacatt
     6541 tcctgctata ataaatgtta ccagtgctgc tatattggac gaaagattga tgaccttagt
     6601 ctgtgcagac gcgctgcggg tatccatttt ggctgcacct gtaaatacaa ggagcagaaa
     6661 tgttcctgta cccggtccgt aaaaaccatc ataacagccg ataaagaaag ctgcgatcac
     6721 agagatcaga aacattttct tataggaagc tgttcctgtt ttttcatctt cccccatgtg
     6781 cttatttttt aatacatgat aagctacaat aggaaggaca acgatcatca tatgccggat
     6841 cacagactct gataccagta aagacattct tgctcccaga acagaacctg tcatggcaca
     6901 gaccgccgat gcaatggcca ttttcatttt cccttttaaa taaccatgtt ttaaaagtct
     6961 caccgcagaa atagaagttc ctatggtaga actcatttta ttggttccaa gagccagatg
     7021 ggccggaacc cctgcgatca gatatccggg gagagaaata agtcccccgc ctccggcaat
     7081 ggaatctaca agacctgcca gaaataccaa cgggcagacg atcagatatg ctgtcatgat
     7141 catttcctct ctttcgtata aaacacgttg ctgttccagc aacggaattt gccgtggctt
     7201 cgcatttaaa tacggcaaat tctcgcagcc actacgttat cgtacggcac tttcgggtcc
     7261 aaaagtgcct acccactaca ataaccaaaa caactcatat cttagcacag tttaaatgca
     7321 ctggcaataa aaggcagctt atttttgcaa taaaactgcc ttcttctgca ttatctatat
     7381 gaacggatga accggtaact tatttcattc cctgttttag atccaatatc cattcaaatg
     7441 agttccttgt tgcattacac tccatcacat caggatatct tctgggaatg ataaaaagct
     7501 gccggttttt acatatgatc catttttaat tttaccttta ctcctgtgct tttacagccc
     7561 agcgaagctt tgtggactta gcccttccgt ttttcgcaca ttcttctgct gacggacgga
     7621 tcacatcctg attgatctca ctgtagatcc cggctttcaa gccttcttta aaggctttct
     7681 ttaccagacg gtcctcaccg gaatggaaag taaggatcgc agctcttcca cctggtttta
     7741 atgcacctgg aagtttatct aaaaatgcgt acaatacttc aaattcactg ttgatatcaa
     7801 tacgcaatgc ctggaaggta cgctggcagg acttctttac tgcctctttt ttctctgctt
     7861 ccggcaggaa agaaagtgct tcttcgatca cttcttttaa ccgggtggtt gtttcaatag
     7921 ggcgaccatg ctttttctcg ttcatcactt ttcttgcaat ctgttctgca tatggctcgt
     7981 ctgagttttc gatcagcatt ccctttaatt cttcaaagtt aagctttttt aaacgttccg
     8041 ctgctgtttc ccccttagac gggtccagtc tcagatccag cggaccttca tttttaaagg
     8101 aaaaacctct gtccggattg tcgatctgca tggaagaaac tcccaggtca gccagaacaa
     8161 agtcaaaacc gcctgcttcc tctgccacct ggtcaatatt ggcaaagttt aaatgcttaa
     8221 tggttaaaat atccggtcca aagcctttat cagccagacg ttttctggtc ttttccattt
     8281 caatggtgtc cacatccagg gcatacatat ggccttcgcc ttttaaacag gctaacatat
     8341 gactggtatg accaccatat cccaaagtag catccagtcc tgtctgtccc ggctgaatgt
     8401 ttaagaaatc caggatttcc tttaccataa tggaaatatg catacctgcc ggtgtacttc
     8461 ccttgctgat gaccttcgca atggtatccc cgtatttttc cggctgcagt tctttgtatt
     8521 tctctttata ggaccttgga tgagtacctt tgtaacggac gcggcgctta tgtggggttt
     8581 ccagattttc tcccccgcct attgtgtctg cggtttcacc gacactgttt atcctttttt
     8641 cttccatctt atttttctcc ttttgatctt aatgttctct cgaaagcctt ttaaacctgc
     8701 ttcttcagaa acatcttaaa atcacaatat tattctgtct gctctcttac cgctgccagg
     8761 aaacgttccc catatttctc atatttaaac tctcccacac cggatacaga cagcatttct
     8821 tccttattta caggctttat aatgcacatg tgagccagtg tcttatcaga aaatacaata
     8881 tacgggggaa ctttttcttc ttttgcgatc tgggcccgaa gttttcgcag tgtttcaaat
     8941 aagttttcct ctgcctctgt aaattcgccg gatccggcca gagcactctt tttcttcctg
     9001 ctcttcttct gcgtttcctg ctcactcttt gtctgctctt ttgccagttt catccatact
     9061 gtttcttcat tatttaatac agatgcagct tttggtgtca gacgcaaaat agcatactca
     9121 tcattggtag cagtaagata gccctccaac tgcagctggt ttaagacctg acgcaaacga
     9181 tacaccggaa ctttcgccaa agagccatac tccagatttt cattcatccc atagccccgg
     9241 atcttggctg tattggcccc atggactgta tcaatgatca cagtcgcgcc atatctctgt
     9301 ctgctggttt caatacagga aagcagtgct tttgccattt ccgttatatc ggtattttca
     9361 aactgtgtga gacaattaga acaatttccg cagtaattac tgccgtattc cccaaaatag
     9421 cggaggatat aatcccgcag gcactcatgg gtaaagcagt aaaaggtcat ttttctgaga
     9481 cgttctctgt cccgcttcat gaccagctgc cttgtcatag gatccaaagc ctcattatcc
     9541 tggttatgat caatgaaaaa ctggttggtg attacgtcct gtcccccata aagaaggatg
     9601 cactccgccg gttccccgtc acgtcctgct cttcctgcct cctggtaata ggattccata
     9661 tttttcggca tattataatg gatgacaaaa cgcacattgg acttgtcaat tcccattcca
     9721 aaggcattgg tagccaccat gatccgcaca ttatcataga taaactcttc ctggttgtgc
     9781 tttcgctctg catcacttaa gcctgcatgg taacgggtaa cggaaaatcc ctctttgatc
     9841 agcttttcac atacttcttc cacgatctta cgggtaagac agtaaacaat tccgctttct
     9901 gacttatgct gttctaacag ctccactaat gcctggtatc gatctttggg agtctgtaca
     9961 ccaaaataca ggttagtccg gtcaaatccg gtggttaaaa cttccggttc ctgaagcata
    10021 agaatgtcta tgatatcgtc tcttacctct gcagtagctg tagctgtaaa agcactgact
    10081 accgggcgtt tgggtagacg tttgataaag tctacgatct ttaaataact gggtctgaaa
    10141 tcctgtcccc actgggaaac acaatgtgcc tcatctacag ccaccatgga aatatttacc
    10201 tgtgggtcta aggcaaaccg aagaaaatcc tccg
//
LOCUS       DBMDUA010000098        10104 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3224_length_10104_cov_3.589023, whole genome shotgun sequence.
ACCESSION   DBMDUA010000098 DBMDUA010000000
VERSION     DBMDUA010000098.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10104)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3224_length_10104_cov_3.589023"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="ACZPCL_08380"
     CDS             <1..1295
                     /locus_tag="ACZPCL_08380"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HIR1162518.1"
                     /translation="MNIYDSSILSKANTQYSNIIETLEAELIIGSAEAYFDQGKVLIA
                     AANPDLMEFYIEPHDLVILGNRYESQLCAIEMGADCIIVCEGAAVSMTIKKIAQERGC
                     TIIATTYDTYTAARLINQSMPISYFMTREHLITFNSDDYIDEIREVMASKRHRDFPIL
                     DKDGRYLGMISRRNLLGAKGKQVILVDHNEKNQAVAGIENAEILEIIDHHRLGTIQTM
                     SPVFFRNQPLGCTATIIYQMYQEAGIKVEPKIAGLLCSAIVSDTLLFRSPTCTPVDEM
                     AARALADIAGIDIEKYAMEMFSAGSNLKDKSDEEIFYQDFKRFTSGKVTIGVGQITSL
                     NGGELDKLKGRMEAFMEKALENNGLDMIFFMLTNILTETTELICEGQGALQLAGKAFH
                     QDIELLEEEGLKEPVLRLPGVVSRKKQLIPELMLAEQE"
     gene            1323..1919
                     /locus_tag="ACZPCL_08385"
     CDS             1323..1919
                     /locus_tag="ACZPCL_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR1162519.1"
                     /translation="MSKEYWKPGNMLYPVPAVMVSCGREGEKPNIITVAWAGTICSDP
                     AMVSISVRKERYSHDIIKETGEFVINLVNKRLVRATDYCGVKSGRDVDKFAETRLTPQ
                     ASRYVKAPGIEESPVNIECKVTEVKELGSHDMFLAKVVGVTIDNQYMDDRGKFNLNAS
                     GLTAYSHGEYFELGKKLGSFGYSVRKPAKKKKRGGNKK"
     gene            1916..2551
                     /locus_tag="ACZPCL_08390"
     CDS             1916..2551
                     /locus_tag="ACZPCL_08390"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR1162520.1"
                     /translation="MKPNITMIGMPSSGKSTVGVLLAKRLGFSFIDVDIVIQEKEGRL
                     LKEIIADEGMDGFLEVENRINAGLEAKMSVIAPGGSVIYGEEAMAHLKEISEIVYLKM
                     SYEEMEKRIGNVVDRGVALKPGFTLRDLYNERVPYYEKYADIVIDEEGKNAGETVDEL
                     RNIIEGMMDQKMIQMFIDEQKRKLEERDRLLSEKDEEIRQLKARIAQLEGK"
     gene            complement(2660..3055)
                     /locus_tag="ACZPCL_08395"
     CDS             complement(2660..3055)
                     /locus_tag="ACZPCL_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162521.1"
                     /translation="MKTKKILWPWAERQVKGKKKASGQPVFGSANMVQDNTGDEVFDN
                     TEIPYDSRPIEISEWFHTFMCMNIPIYGWFYLKKLARQPEHNKMQDFARAYLYYKMVF
                     LAVSLVIVILLLIVGIIGINKLFAYMELL"
     gene            complement(3045..5861)
                     /locus_tag="ACZPCL_08400"
     CDS             complement(3045..5861)
                     /locus_tag="ACZPCL_08400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR1162522.1"
                     /translation="MKDLRKQTHKKMAHGSGHPIATCFGILGWLFHGILSVITAGLII
                     MAIVGLLIYAKVKPELDQCREIAYDKLAQMERSDFSMLSDTVVYDKAGKQIGLINAGH
                     YQYVDISKISMNLQNGYIAQEDRRFKSHGGVDWIATFRAGLALVKHGGHVTQGGSTIT
                     QQVIKNTYLTQERTFTRKIVEILLAPEIEKKYSKADIMEFYCNTNFYGHQCYGVEAAS
                     LYYFGKHASDLAPEEAAILIGISNSPSAYDPVAHPDASKEKRNDVLNSMKEVGYLSPE
                     DYEKAVNSPLKIVQQESEGTDENYQSSYAIHCAALELMKKDNFQFQYIFKDKEDYTQY
                     MDRYTAAYSEKSDLIRSGGFKLYTSIDSTIQSQLQAQIDQSLSGFTELQENGKYALQG
                     AGVIVDNKTNYVVAIVGGRGTEDKFNRAYLSARQPGSTIKPLIDYGPAFDTGEYYPTR
                     MVDDHKWTDGPSNSGGRYFGNVTVREALNRSLNTVAWQILEDIGIDYGLDYLGEMQFQ
                     KLTYVDNNVPSLSIGGFTNGVRVVDMAKGYSTLANGGVYNDRTCIIKIEHEHDGELTK
                     DLKPSAHQVYRDDSAFMLTDVLKGTFTAAYGTGQGLGLDNDMPAAGKTGTTNSSKDTW
                     FCGYTRYYTTAIWVGYDIPRNMPGIYGATYAGRIWKSVMDQIHEGLEPWDWIQPETVE
                     RKVDSKTGMEDYFSTTAQFRAEQSLHEKEQEQLETTLQASVDAFMEKEIGSVEDTYTV
                     KDEYNSITSKLPLLDDGELRASLLEKVESRYDYFKEIIAGMSDEISRYEAVQAEEKKQ
                     AQEQAAKDAEAKRAQEEKAVKKQTFMQALERIENLEYQETDAEDLVSDAIDKLSLVAG
                     DDEEASLSARLQAAISRITTLPTESEWNAAQAEKEAGEAQKESQAQVQVQSQQRQLRS
                     ALSKEQYKWNNAEIYGPGGRGDEN"
     assembly_gap    6013..6022
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     misc_binding    6134..6400
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6610..8325
                     /locus_tag="ACZPCL_08405"
     CDS             6610..8325
                     /locus_tag="ACZPCL_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR1162523.1"
                     /translation="MNTLVIVLIAAVCLFAGYTLYGRWLANKWGIDPKAKTPAVAMED
                     GQDYVPTDGWTVFAHQFSSIAGAGPVTGAIQAAAFGWLPVLLWVIIGGIFFGAVTDFG
                     ALYASVKNEGKSMGLLIEKYIGKTGRKLFLLFCWLFTLIVIAAFADMVAGTFNAYTVK
                     DGVTELSAAAQTNGAAGSISLLFIVFAMVFGVMQKHLHLTGWKETVAGLACTVAALAI
                     GMAFPLVAGKDTWSYLTFAYIFMAAVMPMWLLMQPRDYMTTFMFAGMIIGAVVGLLVA
                     HPTMNLPVYTGFKNAKMGDMFPILFVTVACGAVSGFHSLVSSGTSSKTISNEKDMLKV
                     GYGAMVLESLLAVLALCVAGAAAAADGTAATGTPFQIFSAGVAGFLEMFGIPVYVAQC
                     FMTMCVSALALTSLDSVARIGRMSFQELFSVDDMEHAEGWRKLFCNKYFSTVITLACG
                     FILTKIGYSNIWPLFGSANQLLSALVLITLCVFLKVTGRENKTLFPPLLIMLCVTFTA
                     LVERTIALVNAFGAGTAVFMVEGLQLIIAILLMVLGVIIVYTSGKELFNKKAEVKHSK
                     EGKLA"
     gene            complement(8444..9934)
                     /gene="glpK"
                     /locus_tag="ACZPCL_08410"
     CDS             complement(8444..9934)
                     /gene="glpK"
                     /locus_tag="ACZPCL_08410"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR1162524.1"
                     /translation="MADYIMALDAGTTSSRCILFNKKGEICSLAQKEFTQYFPKPGWV
                     EHDANEIWSTQLGVAVEAMSKISASAADIAAIGITNQRETTIVWDRHTGEPIFHAIVW
                     QCRRTSDYCDSLKAKGLTDTFRQKTGLVIDAYFSGTKLKWILDHVEGARERAQKGELL
                     FGTVETWLIWKLTKGRIHVTDYSNASRTMLFNINSLQWDEDILKELNIPKCMLPKVMP
                     SSCVYGETDSQFFGGPIPISGAAGDQQAALFGQTCFEAGDVKNTYGTGCFMLMNTGNA
                     PVFSKNGLVTTIAWGMDGKVTYALEGSIFVAGAAIQWLRDEMRLIDSAADSEYMATKV
                     PDTNGCYVVPAFTGLGAPHWDQYARGTITGITRGVNKYHIIRATLDSLCYQTNDVLKA
                     MKADSGIDLTSLKVDGGASANNYLMQTQSDILNLTVKRPKCVETTAMGAAYLAGLAVG
                     YWKDKEEVVKNWEIDKTFTPDIPDEKREEMISGWDRAVKCSYGWAR"
BASE COUNT         2664 a         2156 c         2452 g         2822 t
ORIGIN      
        1 acatgaatat ttatgacagc agcattctgt caaaggcaaa tacacagtat tccaatatta
       61 ttgagacact ggaagcagaa ctgatcatcg gaagcgcaga agcttatttc gaccagggaa
      121 aagtactgat cgcagctgca aacccggatc tgatggaatt ttacattgaa ccacatgacc
      181 tggttatttt aggaaaccgt tatgagtccc agctttgtgc cattgaaatg ggagctgact
      241 gtatcatcgt gtgcgaaggc gcagctgttt ccatgaccat taaaaagatc gcccaggaaa
      301 gaggctgtac gatcattgca actacttatg atacttatac agcagcccgt ctcatcaacc
      361 agagcatgcc gatcagctac tttatgacaa gagagcatct aatcacattt aacagtgatg
      421 attatattga cgagatccgc gaagtgatgg caagcaagcg tcaccgcgat ttcccgatcc
      481 tggataaaga tggccgttat ctgggaatga tctcccgcag aaatctgttg ggcgcaaagg
      541 gaaaacaggt tattttagta gatcacaatg aaaagaacca ggcagtagca ggtattgaaa
      601 atgcggagat cttagagatc atcgaccatc atcgtctggg aaccatccag actatgtcac
      661 cggtattttt ccgcaaccag cctttaggct gtactgctac cattatctat cagatgtatc
      721 aggaggccgg tattaaagta gagccgaaga tcgcaggact gttatgcagt gccattgtat
      781 ctgatacact gctgttccgt tctcctacct gtactccggt ggatgagatg gcagcaagag
      841 cgctggctga tattgcaggc attgatattg aaaaatatgc aatggaaatg ttcagtgcag
      901 gaagcaattt aaaggataag agtgacgaag agatcttcta tcaggatttc aagcgcttta
      961 cttccggcaa ggtgaccatt ggtgttggac agatcacttc cttaaatggt ggggaactgg
     1021 ataagttaaa gggtcgtatg gaagcattta tggaaaaagc actggaaaac aatggccttg
     1081 atatgatatt ctttatgctg acgaatatat tgacagagac aaccgaactg atctgtgaag
     1141 gacagggagc cctgcagctg gcaggaaagg cattccacca ggatattgaa ctgttagaag
     1201 aggaaggctt aaaggagccg gtactgcgtc tgccgggagt tgtttccaga aagaagcagc
     1261 tgatcccgga actgatgtta gcagagcagg aatagggatc agatcaagaa aggatagaaa
     1321 aaatgagtaa agaatactgg aagccgggaa acatgctgta tcccgttccc gctgttatgg
     1381 taagctgtgg aagagaaggg gaaaaaccga atatcattac cgttgcctgg gccggaacca
     1441 tctgctctga cccggctatg gtttctattt ctgtgcggaa agagcgttat tcccatgata
     1501 tcatcaaaga gacaggggaa tttgtcatca atctggtaaa taagagactg gttcgggcta
     1561 ccgactactg tggcgtaaaa tccggccgtg atgtggataa atttgctgag acacgtttaa
     1621 cacctcaggc atcccgctat gtaaaggcac ctggtattga ggaaagtcct gtaaatatcg
     1681 agtgcaaggt aacagaggta aaagagctgg gaagccatga tatgttcctg gcaaaggttg
     1741 taggcgtgac cattgacaat cagtatatgg atgaccgcgg caagtttaac ctgaatgctt
     1801 ccggactgac tgcctattcc cacggagaat attttgaact gggtaagaag cttggaagtt
     1861 tcggatattc tgtacgcaaa cctgcaaaga agaaaaagag aggaggaaac aaaaaatgaa
     1921 gccgaatatt actatgatcg gcatgccttc atccggtaaa agtaccgtag gtgtgcttct
     1981 tgcaaagaga ctgggatttt cttttattga tgtggacatt gtgatccagg aaaaagaagg
     2041 aagactgtta aaggaaatta tcgctgacga gggcatggac ggctttctgg aagtagagaa
     2101 ccgcattaat gcaggtctgg aagcgaaaat gtctgtgatc gctcctggcg gcagtgttat
     2161 ttacggggaa gaagcgatgg ctcatttaaa ggagatcagt gagatcgttt acctgaaaat
     2221 gagttatgag gaaatggaaa agcgtatcgg aaatgtagta gaccgtggtg ttgctttaaa
     2281 accgggcttt accttacgtg acctgtacaa tgagcgagtt ccttattatg agaaatatgc
     2341 ggatatcgtc atcgatgaag aaggaaaaaa tgccggtgag accgttgatg agcttagaaa
     2401 tatcatcgag ggcatgatgg accagaagat gatccagatg ttcatcgatg agcagaagcg
     2461 caaactggaa gaaagagacc ggctgttaag tgagaaggat gaagagatcc gtcagctgaa
     2521 agcgcggatc gcgcaactgg aaggaaagta aagtaataag tttcccatct atgaggaaaa
     2581 aatacagata ggggctgccg cttgatcatc tattttggat gattaaacgg cagcccttat
     2641 ttttttaaat cctataacac tataacaatt ccatatacgc aaataattta ttgatcccaa
     2701 taattcccac gatcagaagc aggatcacga tcaccagtga cacggcaaga aagaccattt
     2761 tatagtaaag ataagctctt gcaaagtcct gcattttgtt gtgttctggc tggcgggcca
     2821 gttttttcag gtagaaccag ccgtaaatgg gaatgttcat gcacatgaag gtgtggaacc
     2881 attcactgat ctcaatggga cgggagtcat aggggatttc tgtattgtca aaaacttcat
     2941 cccctgtgtt gtcctgtacc atatttgcag acccgaagac aggctgtcca gaagccttct
     3001 ttttaccttt tacctggcgt tctgcccaag gccacagtat tttcttagtt ttcatcgcct
     3061 ctgcctcctg gtccatagat ttctgcattg ttccacttat actgttcctt acttaaagcg
     3121 gagcgcagct gacgctgctg ggattgtacc tgcacttgag cctggctttc tttctgggct
     3181 tctcccgcct ctttttcagc ctgggcagca ttccactcac tttccgttgg aagagtggtg
     3241 atacgggaga tggcagcctg gagccttgca gaaagggagg cttcctcatc atcacctgca
     3301 acaagggaaa gtttgtcgat agcatcagat accagatctt cggcatctgt ttcctggtat
     3361 tccagatttt cgatccgctc taaggcctgc ataaaggtct gtttctttac tgccttttct
     3421 tcctgggcac gtttggcttc tgcgtctttt gcagcctgtt cctgtgcctg tttcttttcc
     3481 tctgcctgaa ccgcttcata ccggctgatc tcatcgctca taccggcaat gatctcctta
     3541 aagtagtcat accggctttc caccttctct aaaagactgg cgcgaagttc gccgtcatct
     3601 aacagcggca gcttggaggt aatactgttg tattcgtctt ttacagtata agtatcttct
     3661 acagatccga tctccttttc cataaaggca tctacagaag cctgaagagt agtctctaac
     3721 tgttcctgct ctttttcatg aagactctgt tctgcgcgga actgggcagt ggtgctgaaa
     3781 taatcttcca taccggtctt ggagtctact ttgcgctcga ctgtttccgg ctgtatccaa
     3841 tcccagggtt ctaagccctc atggatctga tccataacgg atttccagat ccttcctgca
     3901 taggtggcac catagatacc aggcatattt cttggtatat cataacctac ccagatcgca
     3961 gtagtgtaat aacgtgtata gccgcagaac caggtatcct tactgctgtt ggtagtaccg
     4021 gtctttccgg ctgcaggcat atcattgtcc agtccaagtc cctggccagt gccgtaggct
     4081 gcagtaaatg tgccttttaa aacatctgta agcataaagg cagagtcatc tctgtacacc
     4141 tgatgggcag atggttttaa atctttggtc agttccccgt catgttcatg ttcgatctta
     4201 ataatgcagg tacggtcatt gtatacaccg ccgtttgcaa gggtgctgta gccttttgcc
     4261 atatccacga cccggacgcc gttggtaaaa ccgccgatac ttaaggaagg aacattatta
     4321 tccacataag tcagtttctg gaactgcatt tctcctaagt aatccagacc ataatcaatg
     4381 ccaatgtcct ctaagatctg ccatgcaacg gtgtttaagc ttcggtttaa agcttcacgg
     4441 actgttacat ttccaaaata acgtccgccg ctgttggaag gtccgtcggt ccatttgtgg
     4501 tcatctacca tgcgtgtagg gtaatattca ccggtatcaa aagccggacc atagtcgatg
     4561 agcggcttaa tggtacttcc cggctgtctg gcacttaaat aagctctgtt aaatttgtct
     4621 tctgtgccgc ggccgcctac aatagcaact acatagttgg tcttattatc aacgatgaca
     4681 ccggctccct gaagagcata ttttccattt tcctgcagct ctgtaaagcc ggaaagagac
     4741 tggtcaatct gcgcctggag ctggctctgg atggtactgt ctatggaagt atagagctta
     4801 aagccaccgc ttcggatcag gtctgatttt tcagaataag cggctgtata ccggtccatg
     4861 tactgggtat aatcttcttt atctttaaaa atgtactgga actggaagtt gtctttcttc
     4921 attaattcca gggctgcgca gtggatggca tagctgctct gataattttc atccgttccc
     4981 tcagactcct gctgtacgat cttaagtggg gagtttactg ctttttcgta atcttctggg
     5041 gagagatagc ctacttcttt catgctgtta aggacatcat tacgcttttc tttggaagcg
     5101 tccggatgag ctaccgggtc ataggcagaa gggctgttgc tgataccgat gaggatcgcc
     5161 gcttcttccg gggcaagatc gcttgcgtgt tttccaaaat aatacaggct ggcagcttct
     5221 acgccatagc actggtggcc gtaaaaattg gtattgcagt aaaactccat aatgtcagct
     5281 ttggagtatt ttttctcgat ttcaggggca agaaggatct ccacgatctt ccttgtaaag
     5341 gtacgttcct gggtgagata tgtatttttg atcacctgct gggtaatagt gctgccgccc
     5401 tgggtcacat ggccgccatg tttgacaaga gccagaccgg cccggaaggt ggcaatccag
     5461 tctacgccgc cgtgactctt gaaacgacgg tcctcctggg caatgtagcc gttttgcagg
     5521 ttcatgctta tcttacttat atctacatac tgataatgac ctgcgttaat aagtccgatc
     5581 tgctttccgg ccttgtcata gacaacggta tcagagagca tggaaaaatc ggaccgttcc
     5641 atctgtgcca gtttgtcata ggcgatctca cggcactggt ctaattccgg ttttaccttt
     5701 gcatagatca gaaggccaac gatcgccatg atgataagac cggctgtgat cacagacagt
     5761 atgccatgga aaagccagcc caggatacca aaacaggttg ctatggggtg accggatcca
     5821 tgggccattt ttttgtgtgt ctgcttacgt aagtctttca taatggttta aatcctctct
     5881 ttaagttttc attgaaaatt tttgggaaaa tccacttaaa aaatctgtag ttattataat
     5941 tcatatagaa gaaaattcct agttaaattc gcagtgaaaa cttaaaaaca taggcttttg
     6001 ataggatatt agnnnnnnnn nnggaatata ttttataaaa attagatata gaaattataa
     6061 agaaaatata aaaagaggga attgacaaat ggaaaaaaag gactataatg acaactattc
     6121 aagagttaga cattcaaatg cgaagaacag gacaatgctt ttctgagtat ctacgcatgg
     6181 cagagagctt tcggttggtg agagaaagca gcgcgacaga aaagatatca cctgcaagca
     6241 gtctgtatga aaacaggaaa ctgtaagtaa gacagaccgg ggtcccgccg ttaaaaggga
     6301 gcgtattcgt ccggacagag agtcggagga taggacagag tggtcggtgc aaaccggcaa
     6361 taagaagtgg taacgcggag tttggaactt cgtcttcttg gtgagtatcc atttggggta
     6421 ttcgggaaga tggggttctt tttttactgt gaaagtatcg ccaagcggca gttgtgagag
     6481 tgccgtaggc acggaacaac tgactttcaa ttggtgacga tccgcttata gaaaatggca
     6541 gttggatgtt ttacattgaa ttaacaacag acaatagatc acagattaca tgagaggaga
     6601 gtaaagatta tgaatacact tgtaattgtt ttgatcgcag ctgtatgcct ctttgcaggc
     6661 tacacattat atggccgctg gctggctaat aaatggggca ttgatcctaa ggccaagaca
     6721 ccggcagttg ctatggaaga cggacaggac tacgttccta cagacggctg gaccgttttc
     6781 gcacatcagt tttcatccat cgcaggcgca ggccctgtaa ccggtgccat ccaggcagca
     6841 gcatttggct ggcttccagt tctgttatgg gtcatcatcg gtggtatctt cttcggcgct
     6901 gttacagact ttggtgctct gtatgccagt gttaagaacg aaggaaagtc catgggactt
     6961 ctgatcgaaa agtacatcgg aaagaccgga cgtaagctgt tcttattatt ctgctggctg
     7021 tttacactga tcgttatcgc agcatttgcc gacatggtag caggtacatt taacgcgtat
     7081 accgtaaagg acggtgtaac agaactgtcc gcagcagcac agacaaacgg cgcagccggt
     7141 tccatttccc tgctgttcat cgtatttgca atggtatttg gcgtaatgca gaagcattta
     7201 catcttacag gttggaaaga aacagttgcc ggacttgcct gcaccgttgc agcactggca
     7261 atcggtatgg ctttcccgtt agtagccgga aaagatacct ggtcttatct gacctttgct
     7321 tatatcttta tggcagctgt aatgccaatg tggcttttaa tgcagcctcg tgattacatg
     7381 acaactttca tgtttgcagg aatgatcatc ggtgctgttg taggtctgtt agtagctcat
     7441 ccaaccatga accttccggt ttatactgga tttaagaatg caaaaatggg agatatgttc
     7501 ccaatcctgt ttgtaactgt tgcatgtggt gctgtttccg gtttccacag cctggtttcc
     7561 tccggtacct cttccaagac catttccaat gaaaaggata tgttaaaagt tggatacggt
     7621 gcaatggtac tggaaagctt attagctgtt ctggctctgt gcgtagcagg tgctgctgca
     7681 gctgctgacg gaactgctgc aacaggaacc ccattccaga tcttctctgc aggtgttgca
     7741 ggtttcctgg aaatgtttgg tatcccggtt tatgtagcac agtgctttat gactatgtgt
     7801 gtatctgctc tggctcttac aagccttgac tctgtagcac gtatcggacg tatgtctttc
     7861 caggaactgt tcagtgtaga tgatatggaa catgcagaag gctggagaaa gttattctgc
     7921 aacaagtatt tctcaactgt tatcactctg gcatgtggct ttatcttaac aaagatcgga
     7981 tattccaaca tctggcctct gttcggaagc gcaaaccagc tgttaagcgc actggttctg
     8041 atcactctgt gcgtattctt aaaagtaacc ggaagagaga acaagactct gttcccacca
     8101 ctgctcatca tgctctgcgt aacctttact gcactggttg aaagaaccat tgccctggta
     8161 aatgcatttg gcgcaggaac tgctgtattt atggtagagg gactgcagct gatcattgcc
     8221 atcctgttaa tggtccttgg tgtgatcatt gtatacacat ctggtaagga actgtttaat
     8281 aagaaagctg aagtaaagca cagcaaggaa ggtaagcttg cataagctgg ttcaggaata
     8341 aaaagggttt tggattaaga tatgaaataa aaaatggcgg atatgggatt tgaactctca
     8401 tatctgccat tttttgttgt catgtgtctg ttaagatttt gtgttatctg gcccagccgt
     8461 aggagcattt tacagcacgg tcccagccgg atatcatttc ttcccgtttt tcgtccggga
     8521 tgtccggggt aaatgtttta tcaatttccc agttttttac aacttcttct ttatctttcc
     8581 agtatcccac agccagtccc gcaagataag cagcgcccat agctgtggtt tccacgcatt
     8641 tgggacgttt tacagtaaga tttaagatat cggactgggt ctgcatgaga tagttgttgg
     8701 cactggcacc tccgtctact tttaaggagg tcagatctat gccggagtct gctttcatgg
     8761 cctttaaaac gtcattggtc tggtagcaaa gggagtccaa ggtggcacgg atgatgtggt
     8821 atttatttac acctctggtg atgccggtga tggttcccct ggcatactga tcccagtgag
     8881 gcgcgcccag accggtaaag gcgggaacta catagcagcc gttcgtatca ggaactttgg
     8941 ttgccatgta ttcggagtca gcagcagagt cgataaggcg catttcgtca cggagccact
     9001 ggatggcagc tccggcaaca aaaatagaac cttccagggc ataggttact ttgccgtcca
     9061 tgccccaggc aatggtggtt accaggccat ttttggaaaa gacaggtgca tttcctgtat
     9121 tcatcagcat aaagcagccg gtgccatagg tatttttcac atcccccgct tcaaaacagg
     9181 tctggccgaa taaggcggcc tgctggtccc cggctgcacc actgatcggg atgggaccac
     9241 caaagaactg ggagtctgtt tctccgtaaa cgcagctgga tggcataact tttggcagca
     9301 tacattttgg gatatttaat tcttttaaga tgtcctcatc ccactgcaga gagttgatat
     9361 taaaaagcat ggtgcgggag gcattggaat agtcggtcac atggatacgg ccctttgtca
     9421 gcttccagat cagccatgtt tccaccgttc caaagagaag ttcccctttt tgggcccgtt
     9481 ctctggcgcc ttctacatga tccaggatcc attttaactt ggtgccggaa aaataggcat
     9541 cgatcacaag gccggttttt tggcggaaag tatctgtaag gcctttggct ttcagagagt
     9601 cacaataatc agaggtcctg cggcactgcc atacaatcgc gtggaatatg ggttctcctg
     9661 tatgcctgtc ccatacgatc gtggtctctc gctgattggt aatgccgatg gcggcaatgt
     9721 ctgctgctga cgcgctgatt tttgacattg cttccacagc aacgcctaac tgggtggacc
     9781 agatctcatt tgcatcatgt tccacccagc cgggttttgg aaaatactgg gtaaattctt
     9841 tctgggcaag gctgcagatc tccccttttt tattaaacag gatacagcgg ctgctggtag
     9901 tccccgcatc cagtgccatg atatagtctg ccatgtttct cctcctgtag ataatgtttt
     9961 ccaaaatttg ttttaaattt tattttaata tcattttacc atagcagcaa tgggttcaca
    10021 agcatggtct ggacgttgac atttttataa gaaaaagcct ataatatgag gataagtaca
    10081 tgatatgtac aggacactgg taag
//
LOCUS       DBMDUA010000099        10026 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3239_length_10026_cov_3.212191, whole genome shotgun sequence.
ACCESSION   DBMDUA010000099 DBMDUA010000000
VERSION     DBMDUA010000099.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10026)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10026)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10026
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3239_length_10026_cov_3.212191"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="ACZPCL_08415"
     CDS             <1..1349
                     /locus_tag="ACZPCL_08415"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR1162525.1"
                     /translation="RTNPHGNGFAICAGLEQAIEYIKGLHFDESDIEYLRSLNIFAED
                     FLEYLSTFRFTGDIYAIPEGTVVFPRESLIKVIAPIMQAQLVETALLTIINHQSLIAT
                     KTERIVHAAKGDGVMEFGLRRAQGADAGTYGARAAMIAGCIGTSNVLCGKMFDVPVKG
                     THAHSWIMSFPDELTAFRTYAKLYPSACILLVDTYDTLKSGIPNAIKVFKEMREAGIP
                     LTFYGIRLDSGDLAYLSKKAKKMLDAAGFPDAVISASNDLDEYLIDSLKVQGATINSW
                     GVGTNLITAKDCPSFGGVYKLAAVKDKTTGEFIPKIKLSENAEKITNPGNKTIYRIYD
                     KENGKIIADLICLVGEKFDTNNSLLLFDPQETWKKTLLAPGSYTMRELLVPVFLNGEC
                     VYESPKVMDIQKYCKEELDTLWDESKRLVNPHTVHVDLSNELWQTKQKLLDSYHKK"
     gene            complement(1452..2741)
                     /locus_tag="ACZPCL_08420"
     CDS             complement(1452..2741)
                     /locus_tag="ACZPCL_08420"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005534 - galactose binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIR1162526.1"
                     /translation="MNVCETIKQLNSEKTDAFMHELYGKGAAENKARYEKVLKGFEQA
                     FGDSKEVLLFSSPGRTEISGNHTDHNHGKVLAGSINLDCVGAAAKNYSSHVRIISETY
                     NQDITIDLNDLEPSAKKAGTVDLIKGILKGFETSGYSVGGFNAYITSNVISAAGVSSS
                     ASFEMLLCSMLNAFFNESRMDNVAYAHIGKFAENKYWDKASGLLDQMACAVGGLITID
                     FVEPMTPIVQKIDFDFKAQNHSLIIVQTGKGHADLSAEYSSIPEEMKKVAKYFGKDVL
                     AQISEEKVIQHLPEIRKFAGDRAVLRALHFFEENKRVEAEVAALKEGRFNDFLENITA
                     SGNSSWKWLQNCFTTSNNAEQGITVALALTELFIAQKQRGACRVHGGGFAGVIMAMLP
                     NDLVEEYTKYMEKCLGEGCAYNMSIRPYGAICVSDYL"
     gene            2941..3927
                     /gene="mnmL"
                     /locus_tag="ACZPCL_08425"
                     /gene_synonym="ytqA"
     CDS             2941..3927
                     /gene="mnmL"
                     /locus_tag="ACZPCL_08425"
                     /gene_synonym="ytqA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772478.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification radical SAM protein MnmL/YtqA"
                     /protein_id="HIR1162527.1"
                     /translation="MELEHNWNGRPYHSLDWELKHTFGHKVYKLSLDAGLTCPNRDGT
                     CGTRGCIFCSEGGSGDFAESLKTDVWDQIEAAKGRVSNKTGSDEACNYIAYFQSFTNT
                     YAPVFYLKPLFERAISHPDIAALSIATRPDCLGDDIIDLLKELNSKKPVWVELGLQTI
                     HEKTARFIRRGYELPVFEDALKRLKKAGITVIVHVILGLPGETTDQMKETVTYLADRP
                     IDGIKLQLLHILKGTDLADVYQTDPFHVFSIEEYIDLVIDCITLLPPRITIHRMTGDG
                     PKNLLIAPLWSGNKRLVLNQLHKRFKERGAWQGQCYVSPLIPAALPFRHPQW"
     gene            complement(3887..5209)
                     /locus_tag="ACZPCL_08430"
     CDS             complement(3887..5209)
                     /locus_tag="ACZPCL_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR1162528.1"
                     /translation="MNRKKWICTAAAITGLLLAVIAGLSIRHMKNSSYVSASELAGQK
                     SGSVSEKLEENRNEDLAESSESKNGQPDGKQDQENGAGAEKNENGGNSSAGQNTDEEA
                     AENDSSTGESENDSQESKTESEAEPTDQLVNLVFSGDILLSDHVMNAYKNGGIRSVLD
                     SQFQSVIDESDIFMANEEFPFSTRGTPAEDKQFTFRAAPSLTFVFKELGIDIVTLANN
                     HAMDYGTDALLDTCDTLDKAGIRRVGAGKNLDEAKAPVILEAQGKKIGFLGASRVIPV
                     ASWNATSTKPGMLTTYDPKILVDEIKKLREQCDYLVVYVHWGIERSERPEDYQRSLGK
                     QYIDAGADVVIGSHPHVLQGVEYYKGKPIVYSLGNFIFGSSIPKTALLKVQWDGQASQ
                     LTFVPGTSSAGYTRVLENEVQKADFASYMTGISYGVTIEDDGTVVPQG"
     gene            complement(5232..6092)
                     /locus_tag="ACZPCL_08435"
     CDS             complement(5232..6092)
                     /locus_tag="ACZPCL_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1162529.1"
                     /translation="MTYKIIGDSCLDLTEELKRDPRFQMVPLTLQVGAAQVVDDETFD
                     QKAFIRMVKECPECPKTACPSPEIFKEAYEAADAEAVFVITLSSHLSGSYNSAALAKQ
                     LYEEEMKEKGITSSKKIEVLDSESASAGQLEQALYIRDLCEQGLNFEEVVEKLRKFRD
                     EMHTYFVLESLDTLRKNGRLSGLQAFFATALNIKPVMGADHGVIIKLDQARGINKGLQ
                     RMCDIAIKEAGDTTGKRAVVCHVNNPERGEYVKQELAKRASFKEIVVTNAAGVATVYA
                     NDGGIVLAIG"
     gene            complement(6246..6788)
                     /locus_tag="ACZPCL_08440"
     CDS             complement(6246..6788)
                     /locus_tag="ACZPCL_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1162530.1"
                     /translation="MGTGIMAMELENWICDMANGDKEGLRQFYEETARSVYSFIFSVL
                     KDPHESEDVMQETFLAVWEHAGDYVPYGKPLAWVFTIARRLCYMRLREKKRQETLEDG
                     EIDDTGVPQPQLEQTAERTALLQALDQLNETERQIVLLYAAAGMKHREIAQVLNMPQA
                     TELSKYSRSVKKLQKMLYAS"
     gene            complement(6824..8017)
                     /locus_tag="ACZPCL_08445"
     CDS             complement(6824..8017)
                     /locus_tag="ACZPCL_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR1162531.1"
                     /translation="MTDFLVKCFVKDHDKTEDPQVRTRYGILASIVGICCNLLLFASK
                     LFIGVLVNSVSVMADAFNNLSDAASSIIGFIGVRMAGKPADSDHPFGHGRIEYISAFI
                     VAFLVIQVGFSLFKTSLGKVLHPEEVYFKWISVLILLISVGVKFWLSLFNRKLGSRIN
                     SKVMEATAADAMGDVMTTSAAILSLLALKYLNWNIDGIVGLAVSVAVMYGGLNIAKDT
                     LAPLIGEAIEPEVYEEISNFVEGYEGILGTHDLIVHNYGPSHSMASIHAEVPSNYDPE
                     YSHEIIDRIERDAAKKFNMMLVIHMDPVERDNKRLAQYRSLISRVLMDIDIRLSFHDF
                     RIVGSGKNVCLVFDLVVPREYKASAVGRLKNRINNEVSRREPGCRCSITAENSFRSEK
                     HGSEK"
     gene            complement(8017..8850)
                     /gene="truA"
                     /locus_tag="ACZPCL_08450"
     CDS             complement(8017..8850)
                     /gene="truA"
                     /locus_tag="ACZPCL_08450"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR1162532.1"
                     /translation="MNYKVVMAYDGSRYNGWQKQGNTDKTIQGKLEQILFKMTGEEIE
                     VHGSGRTDAGVHARGQVANFHIDWKKLEVQEKRELEKNAGNQAGQGCKREKCGGKKTP
                     DQIMEYINEYLPEDIAVLSCEVAPERFHSRLSAVRKTYCYQLEMGPKKDVFQRNYYYG
                     LGERLDVSAMKAAAGLLTGTHDFKSFCGNKKMKKSTVRTIEKIEFEQSGSRLHIYFTG
                     NGFLQQMVRILTGTLIEVGQGKCAPDKITRILDAQDRQAAGPTAPAQGLFLVNVGYED
                     K"
     gene            complement(8840..9349)
                     /locus_tag="ACZPCL_08455"
     CDS             complement(8840..9349)
                     /locus_tag="ACZPCL_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR1162533.1"
                     /translation="MKTFMKKWKWKILILVYLCFIYGNSLTPAAISSKESGYFLAKIQ
                     DFLAGAGMDAWWLTEHLVRKAAHFTEYAGLGFLLAMNTGAGIAPVFCLLKGNVTAVLF
                     MPFVDETIQLFVEGRSGQISDVWLDMAGIVTGIMVTAGFMKMTGALKTGRKAAKGKGS
                     GNRSRNNEL"
     gene            complement(9346..9984)
                     /locus_tag="ACZPCL_08460"
                     /pseudo
     CDS             complement(9346..9984)
                     /locus_tag="ACZPCL_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566944.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
BASE COUNT         2594 a         2605 c         1966 g         2861 t
ORIGIN      
        1 accgtaccaa tccccatgga aatggttttg ccatttgtgc cggtctggaa caggccattg
       61 aatatatcaa aggactccat tttgacgaaa gtgatattga atatttaaga agtttaaata
      121 tatttgcaga agacttctta gagtatctta gtactttccg ttttacaggc gatatctacg
      181 ctatcccgga aggtaccgtt gtattcccaa gagagtcttt aattaaagta atcgcaccga
      241 tcatgcaggc acagctggtt gaaacagcac ttcttaccat tatcaaccac caaagtctga
      301 tcgcaactaa aacagagcgt attgtccacg ctgcaaaggg cgatggcgtt atggaattcg
      361 gtctccgccg tgcccaggga gctgatgccg gtacttacgg cgcaagagct gccatgatcg
      421 caggctgtat cggtacttcc aatgtactct gcggaaagat gtttgatgtt ccggtaaaag
      481 gaacccatgc acacagctgg atcatgagtt tcccggatga gctgaccgct ttccgtacct
      541 atgcaaagct gtacccatct gcatgtatcc tgttagtaga tacttatgat accttaaaat
      601 ccggtattcc aaatgccatc aaggtattta aggaaatgag agaagctggc attccgctga
      661 ccttctacgg tatccgctta gacagcggtg acctggcata tctgtcaaag aaagcaaaga
      721 agatgctgga cgcagcaggc ttcccggatg cagttatctc cgcttccaat gacctggatg
      781 agtacctgat cgacagctta aaggtacagg gcgctaccat caattcctgg ggcgttggta
      841 ccaatctgat cacagcgaag gactgcccat cctttggcgg tgtttacaag ttagctgctg
      901 taaaggacaa gaccaccggt gaattcattc caaagatcaa gttatctgag aatgctgaaa
      961 agatcaccaa ccctggaaac aagaccattt accgtattta tgacaaagaa aacggaaaga
     1021 tcatcgcaga tctgatctgc ctggtaggcg agaagttcga tactaacaat tctcttctcc
     1081 ttttcgaccc acaggaaaca tggaagaaaa ccttattagc acctggcagc tacaccatgc
     1141 gcgaactgtt agtaccagtc ttcttaaacg gcgaatgcgt ttatgaatca ccaaaggtaa
     1201 tggatatcca gaaatactgt aaggaagaac tggatacctt atgggatgag tccaaacgtc
     1261 tggtaaatcc tcacaccgtt catgtagatc tttctaatga attgtggcag acgaaacaga
     1321 aattactgga cagctatcac aagaaatgaa agtaataaga atatgatcgc atgataacaa
     1381 acaaggccgt ctgttttcgc agacggccca tttcgtataa atgtctcttt gtttttgact
     1441 ctggtatcac attataaata atcacttacg cagatcgcgc cataaggccg gatgctcata
     1501 ttataagcgc agccttctcc caggcatttc tccatatatt ttgtatattc ttctaccagg
     1561 tcatttggaa gcattgccat gataacacct gcgaaaccgc ctccgtgtac acggcaggca
     1621 cctctctgct tctgggctat aaacagctct gttaaagcaa gggcaacggt aattccctgt
     1681 tctgcgttat tgctagtagt aaagcagttc tgcagccatt tccaggaaga atttccggat
     1741 gctgtgatat tttccaggaa atcattaaaa cggccctctt ttaatgctgc tacctctgct
     1801 tccactctct tgttttcctc aaagaaatga agggcacgca gtactgcacg gtctcctgca
     1861 aacttccgga tctcaggaag atgctggatc accttctctt ctgagatctg ggctaaaaca
     1921 tcttttccaa aatactttgc caccttcttc atctcttccg gaatggaaga atactcagca
     1981 ctcagatcag catggccttt tccggtctgg acaatgatca gactgtggtt ctgtgccttg
     2041 aaatcaaaat cgatcttctg tactatcgga gtcataggct ctacaaaatc aatggtgatc
     2101 aggccgccta ctgcacatgc catctggtca agaagtcctg atgccttgtc ccagtattta
     2161 ttttccgcaa attttccaat atgggcataa gccacattgt ccatacggct ttcattgaaa
     2221 aatgcattta acatggagca taataacatt tcaaaagaag cggaggaact gacaccggct
     2281 gcgctgatca cattgctggt aatataagca ttaaagccgc caacactgta gcctgatgtt
     2341 tcaaatccct ttaaaatacc tttgatcaga tcaacagttc ctgctttctt agcacttggc
     2401 tccagatcat ttaagtcaat agtaatgtcc tgattatagg tttcgctgat gatccgcaca
     2461 tggctggaat aattctttgc agctgcgcct acacagtcta agttgatact gccggccagc
     2521 accttgccgt ggttgtggtc tgtatggttt ccgctgatct ctgtacggcc cggtgatgag
     2581 aaaagaagga cttctttgct gtcgccaaac gcctgttcaa agcctttcag taccttctca
     2641 tatcttgctt tgttttcagc tgctcctttt ccataaagtt catgcataaa tgcatctgtt
     2701 ttttcactat ttaactgttt tatggtctca catacattca tagctgccct ctcctttttc
     2761 tgctgatatg ctattatcag tctaacacaa aagtaacatc tgtcacaata caatattttt
     2821 cgatattgca cattctttca tggacagctg acattttttc tgttataact ggacatacac
     2881 atgacaacgg tccctgcttt gccctgcagg cagaccggaa ttatttttaa ggagtcttac
     2941 atggaactgg aacacaactg gaacggccgc ccctatcaca gtctggactg ggagctgaaa
     3001 catacatttg gacataaagt atataaactg tcactggatg caggtcttac ctgtcccaac
     3061 cgggacggca cctgtgggac caggggatgc attttctgca gcgaaggcgg ttccggagat
     3121 tttgcggaaa gcctgaaaac agatgtatgg gatcagatcg aagctgccaa aggacgggta
     3181 agtaataaga ccggctctga tgaagcttgc aattatatcg cctattttca gtcctttacc
     3241 aatacctacg caccggtttt ttatttgaaa ccactgtttg aacgtgccat cagccacccg
     3301 gatatcgctg ctctttccat tgccacaagg ccggactgcc tgggggatga tattatagac
     3361 ctgttaaaag agttaaacag taagaaaccg gtctgggtgg agcttgggct tcagacgatc
     3421 catgaaaaaa ccgcccgctt tatccgcaga ggttatgaac ttcctgtgtt tgaggatgct
     3481 ttaaaacggc tgaaaaaagc cggcattact gtgatcgtcc atgtgatcct tggacttccc
     3541 ggcgaaacca cagaccagat gaaggaaact gtaacctatc tggcagaccg gcccattgac
     3601 gggatcaagc tgcagctgct ccatatatta aaagggaccg atctggcaga tgtctaccag
     3661 acagatccct ttcatgtgtt ttctatagaa gaatatatcg atcttgtgat cgactgtatc
     3721 acgcttttac cgccccggat caccattcac cgcatgaccg gtgacggacc gaaaaatctc
     3781 ctgatcgcgc ctttgtggag cggaaacaaa cggcttgttt taaatcagct gcataaacgt
     3841 tttaaagaac gtggggcatg gcagggacag tgttatgtgt ccccgcttat ccctgcggca
     3901 ctaccgttcc gtcatcctca atggtaacac cataggaaat acctgtcata taagaggcaa
     3961 agtctgcctt ctggacttca ttttcaagca ctcttgtata tccggcggaa gacgttcccg
     4021 gcacaaaggt aagctggctt gcctgcccat cccactgcac cttaagcagt gccgtcttag
     4081 ggatactgct tccgaaaatg aaattgccca gactgtatac gatcggcttt cccttataat
     4141 attccacccc ctgaagcaca tggggatgac tgccaatgac tacatctgcc ccggcatcta
     4201 tatactgctt tccaagagat ctctggtagt cttccggcct ttcactgcgc tcaatgcccc
     4261 agtggacata taccaccaga taatcacact gctctctcag cttctttatc tcatccacca
     4321 gtattttcgg atcataggta gtaagcatac ccggctttgt agaagttgca ttccaggaag
     4381 ccaccggtat taccctggat gctcccagaa agccgatctt tttgccctgg gcttctaaaa
     4441 taacaggtgc ttttgcctca tctaagttct ttcctgctcc tactctcctg ataccggctt
     4501 tatccaaagt atcacaggta tccagtaaag catctgtccc ataatccatg gcatgattat
     4561 ttgccagtgt cacaatatcg atccccagct ctttaaaaac aaaggtcaaa gacggtgccg
     4621 cccggaaggt aaactgctta tcctctgccg gcgttccccg ggtactgaaa ggaaattcct
     4681 catttgccat gaaaatatcc gactcatcga tcacagactg aaactggctg tccagtacgc
     4741 tgcggatgcc tccgttttta taggcattca tcacatgatc tgataaaaga atgtcaccgg
     4801 aaaagaccag atttaccagc tgatccgttg gctctgcttc agactcagtc ttactctcct
     4861 ggctgtcatt ttctgactca cctgtactgc tatcattttc ggcagcttct tcatccgtgt
     4921 tctgtccagc gctgctattt ccaccgttct cattcttttc tgcaccagca ccattttcct
     4981 gatcctgttt accatctggc tggccatttt tgctttcact gctttctgcc aggtcttcat
     5041 ttctattttc ttccagtttt tcagacactg acccgctctt ctgccccgcc agctcactgg
     5101 cacttacata agaactgttt ttcatatgcc ggatcgataa acccgctata actgccaaca
     5161 gcagccctgt aatagcggcc gctgtgcata tccatttttt tctgttcatt ttttattcct
     5221 gtttaccaat attaaccaat tgccagcacg ataccaccgt catttgcata tacggtagca
     5281 acgccagctg catttgtaac aacgatctcc ttaaaagatg cacgctttgc cagttcctgc
     5341 tttacgtact ctcctctttc aggattattt acatggcata caactgcacg ttttcctgtg
     5401 gtatcccctg cttctttaat ggcaatatcg cacattctct gaagaccctt gttgatacct
     5461 cttgcctggt ctaatttgat gatcacacca tggtcagcgc ccataactgg cttgatattt
     5521 aaggcagttg caaagaatgc ctgcagtccg gaaagtctgc cgttcttgcg taaggtatca
     5581 aggctttcaa gaacaaagta ggtgtgcatc tcgtcacgga attttctcag tttttccact
     5641 acttcttcaa agtttaatcc ctgttcacac agatccctga tataaagggc ctgctctaac
     5701 tgtcctgctg atgcagactc ggaatctaaa acttcgatct ttttagaaga cgtaattcct
     5761 ttttccttca tttcttcttc atacagctgc tttgcaagag cagctgaatt ataacttccg
     5821 cttaaatggc tggaaagagt gatcacaaat actgcctctg catctgctgc ctcatatgct
     5881 tctttaaaga tctctgggga agggcaggca gttttcgggc actccggaca ctccttcacc
     5941 atacggatga aagctttctg atcaaaagtc tcatcatcta ccacctgtgc agcccccacc
     6001 tgcaatgtca gcggtaccat ctgaaacctc gggtcccttt tcagttcttc tgtcagatca
     6061 agacagctgt ctccaatgat cttgtaagtc atgtttctat cctccggtta tccaacatgt
     6121 cgttcgatta tcctaacatg tagtattcat tactacttat tataacagca tactatataa
     6181 aatgcaatgc taaccgaagt ttttattcct gttcataagg caggcctgca gcaaattttt
     6241 cgcaattatg acgcatataa cattttctgc agtttcttca cagagcggct gtatttggaa
     6301 agttccgttg cctggggcat atttaagacc tgggcgatct cccggtgctt catccctgca
     6361 gctgcataca aaagcacgat ctgccgttcc gtttcattta actggtctaa cgcctgtaaa
     6421 agagcggtcc tctctgctgt ctgttctaac tgtggctgtg gcacaccggt atcatcgatc
     6481 tccccgtctt ctaaagtttc ctgccgcttt ttctcccgca gacgcatata gcaaagccgc
     6541 cttgcaatgg taaataccca cgccagcggc tttccatagg gaacatagtc ccctgcatgt
     6601 tcccacactg ccagaaatgt ttcctgcatc acatcttctg actcatgtgg atcctttaaa
     6661 actgagaaaa taaaactgta tacagaccgg gccgtttcct cataaaactg ccgcagccct
     6721 tctttatctc catttgccat gtcacagatc cagttttcca gttccattgc cattatacca
     6781 gttcccatta tcccttctgc caggactaaa atggcggcaa tgcctatttc tccgatccat
     6841 gtttctctga acgaaaactg ttttctgctg tgatggaaca gcggcatccc ggctcccggc
     6901 ggctgacttc attattgatc ctgtttttca gtcttcccac tgccgatgct ttatattctc
     6961 ttggaaccac cagatcgaac accagacaca catttttgcc tgaccctact atgcgaaaat
     7021 catggaacga caggcggata tcaatatcca taagcacccg gctgatcagg gagcggtact
     7081 gggccagacg tttattatcc cgctctaccg ggtccatgtg gatcactaac atcatgttaa
     7141 atttttttgc agcatccctt tcgatccggt caatgatctc atgggaatac tccggatcgt
     7201 agttgcttgg aacttccgca tggatagaag ccatactgtg ggacggacca taattgtgaa
     7261 cgatcaggtc atgggttccc agtattcctt catagccttc tacaaaattg gatatttcct
     7321 cataaacctc cggttcaatg gcttctccga tcagcggcgc caacgtatct tttgcaatat
     7381 tcaatccgcc gtacataaca gccacggaaa ctgccagacc tacaatgcca tcaatattcc
     7441 agtttaaata tttcaaagca agaagggata aaatggctgc actggtagtc attacatctc
     7501 ccatggcatc tgcagccgtt gcctccatca ccttggaatt gatcctgctt cccagttttc
     7561 tgttaaacaa tgacagccag aacttcactc ctacggaaat aagaaggatc agcacggaga
     7621 tccatttaaa atacacttct tccgggtgga gcacttttcc aagagaagtt ttaaacaggg
     7681 aaaatcccac ctgaataacc aggaaagcca caataaaagc agaaatatat tcaatccgcc
     7741 catgcccaaa agggtgatcg ctgtcagccg gttttcctgc catacgcaca ccgataaaac
     7801 cgataatgga cgatgcagca tcggataagt tattaaatgc atctgccata acggacacac
     7861 tgtttaccag aacgccaata aacagcttgg aagcaaacag aagcagattg cagcagatgc
     7921 ctacaatact tgccaggatc ccataccggg tgcgcacctg gggatcttct gttttatcat
     7981 gatctttcac aaaacatttt actaaaaaat cagtcatcac ttatcctcat accctacatt
     8041 taccaggaac agtccctgag ccggggcagt tggtcctgct gcctgccggt cctgtgcgtc
     8101 caggatcctt gttatcttat ccggtgcaca ttttccctgt cccacttcga tcagcgtgcc
     8161 agtaaggata cgtaccatct gctgtaaaaa tccatttcct gtaaaataaa tatgcaggcg
     8221 gcttcctgac tgctcaaact ctattttttc aatggtacgt accgttgatt tcttcatttt
     8281 cttattgccg cagaaacttt taaaatcatg ggttcctgta agaagaccgg ctgctgcctt
     8341 catggcagaa acatccaacc gttctcccag accataataa taatttctct gaaaaacatc
     8401 ttttttcggt cccatttcca gctgatagca atacgtcttt ctcactgctg aaaggcggct
     8461 gtggaaacgc tccggagcca cttcacagga taaaaccgca atatcctccg gcagatactc
     8521 attgatatat tccatgatct gatccggcgt tttctttccg ccacattttt cccttttaca
     8581 gccctgtcca gcctggtttc cggcattttt ttccagttcc cgcttctcct ggacttccag
     8641 ttttttccag tctatgtgaa aatttgctac ctggcccctg gcatggactc ctgcatcggt
     8701 ccggccggaa ccatggactt cgatctcttc cccggtcatc ttaaaaagga tctgttctaa
     8761 ttttccctga atggtcttat ctgtattccc ctgtttctgc cagccattat accggcttcc
     8821 gtcataagcc attaccactt tataattcat tattcctgct cctgtttccg ctgccctttc
     8881 cctttgcagc ttttctgcca gttttcaaag cgcccgtcat tttcatgaaa cctgccgtaa
     8941 ccatgatccc ggtaacaatt cccgccatat ccagccacac atcggatatc tgaccggacc
     9001 tgccttctac aaacagctgt atggtttcat ctacaaaagg cataaaaagt actgccgtca
     9061 catttccttt taaaaggcaa aatacaggcg ctattccagc ccctgtattc atcgccagta
     9121 aaaatcccag tcctgcatat tctgtaaaat gggcggcttt gcgcaccaga tgctctgtca
     9181 gccaccaggc atccattccc gctcctgcaa ggaaatcctg tatcttcgca agaaaatatc
     9241 cgctctcctt agaagaaata gctgcaggag tcagagaatt gccatatata aagcagagat
     9301 ataccaggat caggatcttc catttccatt ttttcatgaa cgttttcatg aattctcctt
     9361 tgagaaaatt ccgctttcat ctactgcatg gatcgcacgc tctaatgcct cacggtcctg
     9421 taccagagcc atacgcacaa agccttcgcc ggatggtccg aaagcacttc ccggtgtcac
     9481 cataacgcct gtcttttcta ccagatccat aacaaaagct tctgaagact tatactgctt
     9541 cgggatcgga gcccatacaa acatagttgc taccggacgg tccatttccc agccaatgga
     9601 gcgaaaaccg tcacagagaa tatctcttct tgcctcatag gctttcatgg tatcataaac
     9661 acagtcctga tcgccggtaa gagctgctac tgctgctgcc tggatcggca ggaacatgcc
     9721 atagtccata ttggatttta acatcttaag atgctttaca acctctttat ttccaaggca
     9781 gaaaccaatt cttgcacctg caaggccata agtcttggaa agtgaattaa attccacacc
     9841 gatctctttt gcgcccgggt aacttaaaaa gcttccgcag gtctttccat caaatacaag
     9901 ctcgctgtat gcattatcat ggagaacaat gatatcgtat ttctttgcaa atgctaccag
     9961 gtctctgtaa aactgcgcag gagcatttcc gcttctgccc ggtaccacaa cacatctgca
    10021 ttaaat
//
LOCUS       DBMDUA010000100         9901 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3270_length_9901_cov_3.558061, whole genome shotgun sequence.
ACCESSION   DBMDUA010000100 DBMDUA010000000
VERSION     DBMDUA010000100.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9901)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3270_length_9901_cov_3.558061"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="ACZPCL_08465"
     CDS             <1..246
                     /locus_tag="ACZPCL_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162534.1"
                     /translation="EIVVVLTRLSKNKRTLTWPFLYELQLLENGGFWYLKYNRKRVKK
                     PWSSAGADGQRNIIRKGGLIYALLTAERRMSDGIKSI"
     gene            194..910
                     /locus_tag="ACZPCL_08470"
     CDS             194..910
                     /locus_tag="ACZPCL_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR1162535.1"
                     /translation="MHYSPQNEECPMALNPFKACVAPRPIGWISTVSRDGIDNLAPFS
                     QFQNLSYDPPMVMIAINQGGDEPDHVHRKDTVNNIESTGCFVYNAVPYDLKEQMNITA
                     SGFAPEEDEFEKAGLHKAESVRVAAKRVAESPIQFECEYMQTIRIPGTMAGGKGTVDL
                     IIGKVVEIHVADEIILPNGKLDYRKLRLIGRAGYWDYGVMEDTFAMKVPGVSAARMAA
                     MEGRKPNEADLEKDKKENSL"
     gene            907..2208
                     /locus_tag="ACZPCL_08475"
     CDS             907..2208
                     /locus_tag="ACZPCL_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HIR1162536.1"
                     /translation="MISFIGMILGLLLFVVLAFKGFNLILSAVASSCLMFVFSGISIL
                     DGLKEFYLPGLAGFLQSYFLLFFLSALMGKLMSDGGNAKKIALSLSGLIKKSKNNQKF
                     FCVLLVPVLYFILCYVGISGFVVVFTVLPIAKTIYEETDTPWRLYCCAGAQTVSAAYL
                     AGSIQAGNVYATDICGTTTMAGWKLSIVAVVTFWAVSLVFLWTMLKITQKKGEAFLPS
                     GEGIRMANLDEGLSEDCLPGLLPSILPLAVVLVLSAGFQVYVVKALFAGCILTVITGH
                     KNLLPKLKTSLSQGITAAYGPILSVSATYAIGAVVKNIEGFTYFQNMLSALPHLMSGS
                     LMGLLAAFIMASVAAPIPAFGSHMLDQYTLAGLSAGNAHRMMMLTSFTSIAPHNAGIP
                     NAAAVLRMPYAECLKMYMMSTYIPGFITLFVSILCIKMGIV"
     gene            2282..3496
                     /locus_tag="ACZPCL_08480"
     CDS             2282..3496
                     /locus_tag="ACZPCL_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014564.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR1162537.1"
                     /translation="MSAHKPLEGVKVVELASFVAAPTAGRMMAEMGADVIRVESTAGD
                     PWRFYGANCGLPITDEENPLFDIYNLGKKDIQLNTKTPEGKEILFHLLDEADVFITNN
                     RLKSLVRSGLDYESLKDRFPKLIYGLVTGYGQTGPDVDAPGYDGVAFFSRSGMLADMA
                     EPSGYPACAPGCIGDGATGAALFGGICAALFDRVRTGKGDFVEISLFGNAVWLCGTMS
                     AFEQYGYHYPKKRSEMGALYTFYKCKDGEWLHLAVTQHDRYWKPLAEALNVPELAEDE
                     RFANAALISKNRAQLIPLLEEAFSHFNYNEIAARLKERDIVFDRMRHYRELATDPQAV
                     ANGFVKEYIYERGHPFMMSMPPVHMRSVGETGPGRGPKPGEHTDEILKQLGCSEDEIV
                     HLKEAGAVKQHS"
     gene            3659..4633
                     /locus_tag="ACZPCL_08485"
     CDS             3659..4633
                     /locus_tag="ACZPCL_08485"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR1162538.1"
                     /translation="MKTRMTELLGIKYPIMQGGMQHLGVPELAAAVSEAGGLGTINVT
                     IYPDPEDLRKAIKEVKAKTDKPFAVNISLIPSLHPGQELFDQVKVILEEGVTAVETAG
                     ASPQELADVLNAHKDQVKWIHKAACVKHAKKAESMGADLITMAGFEVAGHPHTDGVGT
                     MVLANRTAREVKVPVLAAGGIADGRGLLAALSLGCEGVVMGTRFVASKECWISENHKN
                     WIVNANENQTVLCQKSIKNMVRVANNEAARKCLELEAKGATLQELMPVISGKLGKAAY
                     ADGNVDGGMFAVGPAAGLIHEVESCKEIIDSIMAEAEEGLKRLNSLGL"
     gene            4692..5897
                     /locus_tag="ACZPCL_08490"
     CDS             4692..5897
                     /locus_tag="ACZPCL_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014564.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR1162539.1"
                     /translation="MGKALEGIRVLDFTTMAAGPTAAAMLADYGAEVIKIERPGRGED
                     GRKFPPMVDGESLTYCWFNRGKKSLAVDMKDPEGLELVKKLIPTAQVILENFRPGTMK
                     KFGLDYESVCKVKPDMVYVSLTTYGQTGRLISKPGYDIIAQAMSGIMSVTGEADGAPQ
                     KSGIPLGDMVGALNMFASTMTALYHWKDTGEGQYVDVSLLRSLLYMNTPLIHCNLGKE
                     RMSKRQGNHHPTMCPYGVFHCKDGDIVMAASGKRIWDSLCDLMGRPEWKEDPEYQEVG
                     DRARHQKVLIPLFNEWLTNSFSGQDEALAALDAAGIPCCKVQDEYQAWSNEDFRENGW
                     ISEAPTMPDMKMKTFYTRTGNCSMSKTAPEFKRAPLLGEQNVELFKELGYSEEKIKEL
                     EERWPHQNL"
     gene            6029..7171
                     /locus_tag="ACZPCL_08495"
     CDS             6029..7171
                     /locus_tag="ACZPCL_08495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR1162540.1"
                     /translation="MQNGLFNVTEEQQSLVELIRDFMTKEIKPHVLEWEKEGHYPQEI
                     IEQGIEMGLHMLQVPEEYGGMGLDTTTTAMMIEEGAKVESTFMGMFNVTSMGGKIVMA
                     AGNEEQKQYYSGLLQKGYLSSFCLTEPDAGSDSAAVRTTAVRKGDSYVINGTKMFITN
                     ASLASVFLVVATLDPSKGSKGLATFIVEKDRPGVTVGKKIEKIGMRLSNTAEVIFDNV
                     EIPAFNLIGTEEGGFANAMKVLTASRPIIAASSLGACQLARDLAIQYSKERIAFGKPI
                     CAKQMIQEKIANMEMLIQAGRGLVYNATMLLDQGKPCNKEASIAKCFVTEAYGKITDD
                     ALQIFGGYGLCDEYLISKLYRDARVNRIIEGTNEIQRVVIAKSLLR"
     gene            7375..8691
                     /locus_tag="ACZPCL_08500"
     CDS             7375..8691
                     /locus_tag="ACZPCL_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HIR1162541.1"
                     /translation="MISLLGIVIGLALFILLAYMGYNTIIVAIASAMVVAVFGGMNPF
                     VALTEAFMPKTVGFMQGYFLIFLFSALFARFMGDSGAAPSIAVKVARIAKKAKNPDMQ
                     RWLAVMVLPLIQLILTYGGVNVFVVVFILVAIARSLFKDLNIPWWMYSCSCLGSSTVT
                     IGMLPGSPQIQNIIPMQYFGTTTMSAPVLGILSSIITFAIGSLYIWWQCKRTNKKGEG
                     FFPTGELIDKEQLVEASVENEKPLWQCLLPMIVVIVVLNVLKQSAVIALCSGCVVAWL
                     LYDPRKQDLKKIFSGAMPQAIMPLITVCCASGFGGVVAAVPGFQTIVGSLNNFGTSPF
                     MIVLVVNICSGICGSASSGENIALQNFADRFIATGIPGPQLHRLVAMSSIGLDTLPHS
                     AGIITMLSTTKLTHRQAYINNFILSLVLPIVMACFAAVMISAGFYL"
     gene            complement(8946..9749)
                     /locus_tag="ACZPCL_08505"
     CDS             complement(8946..9749)
                     /locus_tag="ACZPCL_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012596.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR1162542.1"
                     /translation="MYQYVKTYIENGAGYIILNDPKKLNALSQPMAAELSEVLEQFRF
                     DPQIRVIVLRGEGGRFCAGGDITSMKKRVDCYAAGIPAPTQTKTNMANFNHLVLYIRQ
                     IEKPVVAWIEGACAGGGMSLAMACDFAIAEENTKMSFAFSSIGLAPDMGSSVLLTRRV
                     GPARAADLFMTGRRFTGKEAADMGIITRAVPAEELEENVKKQITALANGPTLSYAEIK
                     GGINRIMYPDLHQCMSLEADYADRLTHSADHAEAVNAFLEKRKPVFQGK"
BASE COUNT         2812 a         2007 c         2499 g         2583 t
ORIGIN      
        1 gaaattgtgg ttgttttaac cagattgtct aaaaataaac ggactttgac atggcctttt
       61 ttgtatgaat tgcagttgtt ggaaaatggg ggattttggt atcttaaata caacagaaaa
      121 agggtgaaaa agccctggtc atccgccggg gcggatggtc agagaaacat aatacgcaaa
      181 gggggactta tttatgcatt actcaccgca gaacgaagaa tgtccgatgg cattaaatcc
      241 atttaaggcc tgtgtagctc caagaccgat tggctggatc tccactgtaa gcagggacgg
      301 aattgataat ctggcgcctt tcagccagtt ccagaacctt tcctacgatc cgcctatggt
      361 catgatcgct attaaccagg ggggagacga gccggatcat gtacaccgca aggatactgt
      421 aaacaatatt gagtctacag gctgctttgt atataacgca gttccgtatg atctgaagga
      481 gcagatgaac attacagcca gcggttttgc gccggaagag gacgaatttg aaaaagccgg
      541 actccacaag gcagagtctg taagagtggc agcaaagcga gtagcagaat cacccatcca
      601 gtttgagtgt gaatacatgc agaccatccg tattcccgga actatggcag gcggaaaagg
      661 cacggtggat ctgatcatcg gaaaagtagt ggagatccat gtggcagatg agatcatcct
      721 tccaaacgga aagctggatt acaggaaact gcgcctgatc ggcagagccg gatattggga
      781 ttacggggta atggaagata cctttgcaat gaaggttccg ggagtcagcg cagcccgtat
      841 ggcagccatg gaaggcagaa agccaaatga ggcagacctt gaaaaagaca aaaaggaaaa
      901 cagcctatga tcagcttcat aggaatgatc ctggggcttt tattgtttgt agtgctggca
      961 tttaaaggtt ttaacctgat cttgtcagca gttgccagca gctgtcttat gtttgtgttt
     1021 tccgggatca gtattttaga cggcttaaaa gaattttatt tgccgggact ggcaggtttt
     1081 ttacaaagct atttcctcct gtttttctta agtgccctta tgggaaagct gatgagtgat
     1141 gggggaaatg caaaaaagat tgccttgtcc ttatcagggc tgatcaaaaa aagcaaaaac
     1201 aaccagaagt ttttctgtgt gcttttagtt ccggttttgt attttatctt atgttatgtg
     1261 ggaatttccg gttttgtggt ggtgtttacg gtacttccta ttgcaaagac catttatgag
     1321 gaaacagata ccccctggcg gctgtactgt tgcgccgggg cacagactgt aagcgccgct
     1381 tatctggccg gttctattca ggcgggcaat gtatatgcta cggatatctg cggcacaacc
     1441 accatggcgg gctggaagct ttccattgtt gcagtggtca cattctgggc ggtatccctg
     1501 gtgtttttat ggaccatgtt aaaaataaca cagaaaaagg gggaagcgtt cctgccatcg
     1561 ggagaaggaa tccgtatggc caatctggat gaaggtttat cggaagactg tcttccggga
     1621 ctgcttccaa gtatccttcc tttagctgta gtgttggtgc tgagtgccgg atttcaggta
     1681 tatgtagtaa aggcattatt tgcaggctgc atccttactg ttattacagg acataagaac
     1741 ctgctgccaa agctaaagac aagcctttca cagggcatca ctgctgccta tggccccatt
     1801 ttaagtgttt ccgctaccta tgccattggc gcagtggtaa aaaacataga aggttttacc
     1861 tattttcaga acatgctgtc tgctctgcct catctgatga gcggaagcct tatgggactt
     1921 ttagcagcct ttatcatggc atctgtagca gcaccgatcc cggctttcgg ttcccatatg
     1981 ttagatcagt acacactggc aggacttagt gcaggcaatg cccaccgcat gatgatgctt
     2041 acttctttta ccagtattgc gcctcataat gcgggaatac ccaatgcagc ggcagtgctt
     2101 cgcatgccct atgcagaatg tctgaaaatg tatatgatgt caacttatat accaggcttt
     2161 attactttgt ttgtatctat tttatgtatt aaaatgggga tcgtctgacc ggtgatcttc
     2221 gtataagatc aggcacagaa acatcccagg agaacatttt taggatcagg aggaaaagat
     2281 catgagcgca cacaaaccat tagaaggagt gaaagtggtg gaactggcca gttttgtggc
     2341 agcgccgaca gcaggaagaa tgatggcaga aatgggagcg gatgtgatcc gcgtggaaag
     2401 cactgcaggt gatccatggc gtttttacgg tgccaactgt ggacttccca ttacagatga
     2461 ggaaaatcct ctttttgaca tttacaatct gggcaaaaag gatatccagt tgaacaccaa
     2521 gactccggaa gggaaagaga ttttgttcca tcttttagat gaagcggatg tatttattac
     2581 caataaccgt ttaaaatccc tggtaagatc cggtcttgat tatgagtcgt taaaggaccg
     2641 tttcccaaag ctgatctatg gcctggtaac aggatacgga cagacaggcc cggatgtgga
     2701 tgcaccggga tatgacggtg ttgccttttt ctcaagatcc ggaatgttgg cagatatggc
     2761 agaacccagc ggctatcctg catgcgcccc gggctgtatc ggagatggag ctacaggagc
     2821 tgcgcttttt ggcggtatct gtgcagcgct ttttgacagg gtgcgtacag gaaaaggcga
     2881 ttttgtagaa atttctcttt ttggcaatgc agtatggctt tgcggtacga tgtctgcttt
     2941 tgagcagtat ggttatcatt atcctaaaaa gagatcggaa atgggggctc tttatacttt
     3001 ttataaatgc aaggacggag aatggctcca cctggctgtt acccagcatg accgctactg
     3061 gaaaccgtta gcagaggcgt taaatgtgcc ggagctggca gaagatgagc ggtttgccaa
     3121 tgctgcgctg atctctaaaa acagggccca gctgatcccg cttctggagg aagcctttag
     3181 tcattttaac tataatgaga ttgcggcaag actaaaagaa agggatatcg tctttgaccg
     3241 tatgcgccat tacagggagc tggcaacaga tcctcaggct gtggcaaatg gctttgtaaa
     3301 ggagtatatc tatgaaagag gccatccatt tatgatgtcc atgccgccgg ttcacatgag
     3361 aagtgtggga gaaaccggcc cgggacgggg gccaaagcca ggagaacaca cagatgagat
     3421 cttaaaacag cttggctgtt cagaggatga gatcgttcat ttaaaagaag ctggagccgt
     3481 aaaacagcac tcttaaaagc ttttggttaa tataaccatt acttttaaga aagcacctat
     3541 aaaaaaattg tatgaaccgc tgtatcttta cagcggttca aatgttattt taataacaag
     3601 caatgcattc atgcggggaa accgtgtgta ttttataaag taaaggagag gcagatcaat
     3661 gaaaacaaga atgacagaac ttttagggat taaatatccg atcatgcagg ggggaatgca
     3721 gcatctggga gttcctgaac tggcagctgc cgtatcagaa gcaggcggtc ttggaaccat
     3781 caatgtaact atttatccgg atccggaaga tttaagaaag gccatcaaag aagtaaaggc
     3841 aaaaacagat aaaccatttg cagtaaatat ttccctgatc ccaagtctcc atccgggaca
     3901 ggagcttttt gaccaggtta aggtgatcct ggaagaagga gtcacagcag tagaaacagc
     3961 aggcgcaagc cctcaggagc tggcagatgt attaaatgcc cataaggatc aggtaaaatg
     4021 gatccataaa gcagcttgtg taaagcatgc aaagaaagca gagagcatgg gtgctgatct
     4081 tattaccatg gctggctttg aggtagcagg acatcctcat acagatggtg tgggaactat
     4141 ggtactggca aaccgcacag caagagaagt aaaggttcct gtactggcag caggcggtat
     4201 tgcagatggc agaggcttac tggcagcatt aagccttgga tgcgaaggtg ttgtaatggg
     4261 aacacgtttt gtagcaagta aggaatgctg gatctctgaa aaccataaaa actggatcgt
     4321 aaatgcaaat gagaaccaga ctgttctttg ccagaaatct attaaaaaca tggtccgcgt
     4381 tgcaaataat gaagcagcaa gaaagtgcct ggagttagag gcaaagggag ccaccttaca
     4441 ggaactgatg cctgttatat caggaaaact tggcaaagcc gcttatgcag acggaaatgt
     4501 agatggcggt atgttcgctg tcggaccggc tgctggcctg atccatgagg tagaaagctg
     4561 caaggagatc attgactcca ttatggcaga ggcagaggaa ggtttaaaac gtttaaacag
     4621 ccttggttta taagataata agcctgttat gaaaataact ggaaagtaat ttggaaagga
     4681 agaagataaa tatgggaaaa gcactggaag gaatccgcgt tctggatttt acaacaatgg
     4741 cagcaggacc tactgcagca gcaatgttag ctgattacgg cgcagaagtt attaaaattg
     4801 aacgtccggg aagaggagaa gacggcagaa agtttccgcc tatggtagac ggtgaaagcc
     4861 ttacatactg ctggttcaac cgtggaaaga aatcactggc agtggatatg aaggatccgg
     4921 aaggactgga actggtaaag aaactgatcc ctactgcaca ggtgatctta gagaatttcc
     4981 gtcccggaac catgaagaaa tttggtctgg attatgaaag tgtatgcaag gtcaaaccgg
     5041 atatggtata tgtttccctt accacttatg gacagacagg acgacttatt tccaagccag
     5101 gatatgacat tatcgcgcag gcaatgtccg gtatcatgag tgttacaggt gaagcagacg
     5161 gagcacccca gaaaagcggc atcccgttag gagatatggt tggtgcttta aatatgtttg
     5221 caagcaccat gactgcttta tatcactgga aagatacagg ggaaggccag tatgtagatg
     5281 tatccttact tcgttccctg ttatatatga acacacctct gatccactgt aatctgggca
     5341 aagagcgcat gagtaaacgt cagggaaatc atcatcctac tatgtgtcca tatggagtat
     5401 tccactgcaa ggacggtgat atcgttatgg cagcttccgg aaagcgtatc tgggacagct
     5461 tatgtgacct gatgggaaga cctgagtgga aggaagatcc tgaatatcag gaggttggtg
     5521 acagagcacg tcatcagaag gttctgatcc cattatttaa cgaatggctg accaacagtt
     5581 tctccggaca ggacgaggca cttgcagctt tagatgctgc cggtatccca tgctgcaagg
     5641 ttcaggatga gtaccaggca tggtcaaacg aagatttccg tgaaaatggc tggatttcag
     5701 aagcacctac tatgccggat atgaagatga agaccttcta tacaagaacc ggcaactgca
     5761 gcatgtcaaa gacagcgcct gagtttaaga gagcgccatt actgggcgag cagaatgtgg
     5821 agttgttcaa ggaattaggc tatagcgaag aaaagatcaa agagctggaa gagcgctggc
     5881 cacaccagaa tttatagttt tcagaacagt gaaaatatag ttttcaaaac agtgaaaaat
     5941 ctggcgcaga acttacggaa ggcttgcctg taagcaaagt tttgtgtcag attttacatc
     6001 ggagattaaa agtaaggaga tcaaccatat gcagaatgga ttatttaatg taacagaaga
     6061 gcagcagagc ctggtagagc tgatccgtga ctttatgacc aaagagatca agccgcatgt
     6121 gctggaatgg gaaaaagaag gccattatcc acaggagatc atagaacagg gtattgaaat
     6181 gggactgcac atgctgcagg taccggaaga gtacggcggc atgggactgg acaccacaac
     6241 tacagctatg atgatcgagg aaggcgcaaa agttgaaagt acctttatgg gtatgttcaa
     6301 tgtaaccagc atgggcggaa agatcgttat ggcagcagga aatgaagagc agaagcagta
     6361 ttattccggc ctgctccaga agggatattt aagctctttc tgcctgacag agcctgatgc
     6421 aggttctgac agtgctgctg tacgtaccac ggctgtgcgc aaaggtgata gttacgttat
     6481 taacggaaca aaaatgttca ttaccaatgc ttctttagca agtgtattcc tggtagtggc
     6541 aacactggat ccatctaagg gttctaaggg actggctacg ttcattgtag aaaaagaccg
     6601 tccaggtgtt actgtaggaa agaagatcga aaagatcggc atgcgtcttt ccaatacagc
     6661 ggaggttatt tttgacaatg ttgagatccc ggcatttaat ctgatcggaa cagaagaagg
     6721 cggttttgcc aatgccatga aggtccttac cgcttccaga ccgatcattg cggcttccag
     6781 ccttggcgca tgccagttag caagagacct ggcaatccag tattccaagg aaagaattgc
     6841 ttttggaaaa ccaatctgcg caaaacagat gatccaggaa aagattgcca acatggaaat
     6901 gctgatccag gccggccgcg gactggtata caatgctacc atgcttcttg accagggcaa
     6961 gccatgcaac aaagaggctt ctattgctaa atgctttgta acagaggcat atggaaagat
     7021 cacagatgac gcactgcaga tttttggcgg atatggtctt tgcgatgaat atctgatctc
     7081 caagttatac cgtgatgccc gtgtgaaccg tattattgaa ggaaccaacg agatccagag
     7141 agtggttatt gccaagtctt tactgcgata aaaaactaac aggtcggttt aaccagaaaa
     7201 taacaaaaat catttttcga tttttgtgcg tagtgacata gatggtttga tctgagtttg
     7261 ttattattat gtcatagtca gttacatcat aataaatcgt ttcaagacct gctttatgcg
     7321 gaagaacagg gggtgtggta cctgaaacga taaataaacg agaggggtaa tgatatgatt
     7381 agtttactgg gtattgtgat cggacttgcg ttgttcatcc tgttggctta catgggttac
     7441 aacacgatca tcgttgccat tgcttcggct atggtagtag cagtatttgg aggaatgaat
     7501 ccattcgtag cattaactga ggcatttatg ccaaagacag ttggattcat gcagggatat
     7561 ttcctgatct tcctgttcag tgcattattt gcacgtttca tgggcgattc cggtgcagca
     7621 ccttctattg cagttaaggt tgcacgtatt gcaaaaaaag caaaaaatcc ggatatgcag
     7681 cgttggctgg cagttatggt acttccgctg atccagctga tcttaaccta cggcggcgtt
     7741 aacgtattcg tagtagtatt catcctggta gcaattgccc gttcactgtt taaggatctg
     7801 aacattccct ggtggatgta ctcctgctcc tgtcttggtt cttccacagt gaccatcggt
     7861 atgcttcctg gttccccgca gattcagaac atcatcccaa tgcaatattt tggaacaact
     7921 accatgtcag cacctgttct tggtatctta agctccatta ttacatttgc tattggttcc
     7981 ctgtatatct ggtggcagtg caagcgtact aacaagaagg gtgaaggatt cttcccaact
     8041 ggcgaactga tcgataaaga gcagttagta gaagcaagcg ttgagaacga aaaaccatta
     8101 tggcagtgct tacttccaat gatcgttgtt atcgttgtat taaatgtatt aaagcagagt
     8161 gcagtgatcg ctctttgcag cggctgcgta gttgcatggc tgttatatga tccacgcaag
     8221 caggatttaa agaagatctt ctccggcgct atgccacagg ctatcatgcc tctgatcact
     8281 gtttgctgtg catcaggctt tggtggtgtt gtagcagctg ttcctggatt ccagactatt
     8341 gttggcagct taaataactt tggaaccagc ccatttatga tcgtattagt agttaacatt
     8401 tgttcaggta tctgcggttc cgcttcttct ggtgagaaca tcgcccttca gaactttgct
     8461 gaccgtttta tcgcaacagg aattcctgga cctcagttac accgactggt tgcaatgtct
     8521 tccatcggtc tggatacact gccacacagt gcaggtatca tcactatgct gtctacaacc
     8581 aagctgaccc acagacaggc ttacataaac aactttatcc tgtctctggt actgccgatc
     8641 gttatggcat gcttcgcagc tgttatgatt tccgcaggtt tctatctgta agcacaggtt
     8701 aaggatacat aaaaacgaaa aatgaaatcc ccacaacatg tcgtaccttc cgcatacggc
     8761 atgtatataa aactcctgga aaatgctaga taaaagcgtt ttccgggagt ttttatgttt
     8821 cgctaactca ggttttgcaa ataaaaaggg acctgcaaag aacaggtcca gtgtgctggt
     8881 gaaggtctca cctgcgtcag caggcagata agcgtgccaa cgcaaagacg tgagtttacg
     8941 atacattatt tcccttggaa tacaggcttt ctcttctcta aaaatgcatt taccgcttct
     9001 gcatggtcgg cagaatgggt cagacggtct gcataatccg cttccagact catgcactgg
     9061 tgcagatccg ggtacatgat gcggttgatg ccacctttga tctctgcata ggaaagagta
     9121 ggtccgtttg ccagggcagt aatctgtttt tttacatttt cttccagttc ttcagctggt
     9181 acagcacggg tgatgatgcc catatcagct gcttcttttc ctgtaaaacg tctgcctgtc
     9241 ataaaaagat cggcagctct tgcaggacca acgcggcggg taagaagaac ggagcttccc
     9301 atatccggtg ccagtccaat agaagaaaag gcaaaggaca tttttgtgtt ttcctctgca
     9361 atggcaaagt cacaggccat ggcaaggctc attccgccgc ctgcgcaggc accttcgatc
     9421 caggcaacta caggcttttc aatctggcgg atgtaaagga ctaggtggtt gaaattagcc
     9481 atattggtct tggtctgggt tggagcaggg ataccggctg cataacagtc cacacgcttc
     9541 ttcatagaag taatgtcacc gcctgcacag aaccttccgc cttcgccccg gagcacgatc
     9601 acgcggatct gtgggtcaaa gcggaactgc tctaaaactt cggaaagctc cgctgccata
     9661 ggctggctta aggcatttaa ctttttgggg tcattgagga tgatataacc tgcaccattt
     9721 tcgatgtaag ttttaacata ctgatacata aaaatacctc ctgttatatt tttcaccaga
     9781 gcaaaccaaa tcagtccagt gagaaatcat ttctgctcta agtataaggc aaaagcatgc
     9841 agattgcacg gtacaaacta tggataatcc tacgaaaata acaggtggta aggggaatgt
     9901 t
//
LOCUS       DBMDUA010000101         9762 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3322_length_9762_cov_2.757064, whole genome shotgun sequence.
ACCESSION   DBMDUA010000101 DBMDUA010000000
VERSION     DBMDUA010000101.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9762)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3322_length_9762_cov_2.757064"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            354..638
                     /locus_tag="ACZPCL_08510"
     CDS             354..638
                     /locus_tag="ACZPCL_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR1162543.1"
                     /translation="MKLVPLFDKVVLKQLVAEETTASGIVLPGAAKEKPQQAEVIAVG
                     PGGVVDGKEVTMQVKAGDKVIYSKYSGTEVELEKEKYVIVKQSDILAVVE"
     gene            749..2371
                     /gene="groL"
                     /locus_tag="ACZPCL_08515"
     CDS             749..2371
                     /gene="groL"
                     /locus_tag="ACZPCL_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721716.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR1162544.1"
                     /translation="MAKQIKYGVEARKALEAGVNQLADTVRVTLGPKGRNVVLDKSFG
                     APLITNDGVTIAKEIELADPFENMGAQLIKEVASKTNDVAGDGTTTATVLAQAMVNEG
                     MKNLAAGANPIILRKGMKKATDAAVEAIKKMSQPVGGKAQIARVAAISASDDEVGTMV
                     ADAMEKVSKDGVITIEESKTMKTELDLVEGMQFDRGYLSAYMCTDMDKMEANLDDPYI
                     LITDKKISNIQEILPILEQIVKMGARLLIIAEDVEGEALTTLIVNKLRGTFNVVAVKA
                     PGYGDRRKEMLQDIAILTGGTVISEEVGLELKDATMEQLGRAKSVKVQKENTVIVDGN
                     GSKEMIAARVAQIRKQIGETTSDFDREKLQERLAKLAGGVAVIRVGAATETEMKEAKL
                     RMEDALNATRAAVEEGIIAGGGSAYAHATVDVQKVVDTLEGDEKTGAKIILKALEAPL
                     FHIVANAGLEGAVIVNKVKESPVGEGFDAYNETFVNMIEAGILDPVKVTRSALQNANS
                     VASTLLTTESVVATIKEPAPAAPAAPDMGGMY"
     gene            2564..4351
                     /gene="ilvD"
                     /locus_tag="ACZPCL_08520"
     CDS             2564..4351
                     /gene="ilvD"
                     /locus_tag="ACZPCL_08520"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR1162545.1"
                     /translation="MKLHSQEVRKLAPENDPLKIGMGWSVADLDKPQILVESSFGDSH
                     PGSAHLNQFVEEGVKAVNDNGGKAARYYVTDICDGIAQGHDGINYSLAHRDMMTNMIE
                     VHGMASGFDGGMFIASCDKSVPAILMGIGRLKDMSAIVVTGGVMEAHTITPKYVENDP
                     ACAINELLTLEQIGKFDAQEKRGEIPEEQLNYYKHNACPSCGACSFMGTASTMQIMAE
                     ALGLMLPGTALMPATAPELKQAAYDAGKQLMKLVEKGIKAKDIVTMKSFENAIMVHAA
                     ISGSTNATMHIPAIAHEFGFEVDAETFDRMHRGAHYLLNIRPSGDWPAQYFYYAGGVP
                     RVMEEIKDMLHLDVMTVTGKTLGENLEELKKNGFYDHCDELLREKGKLLGREIKRTEI
                     IHAFDDAKGTDGSIAILKGNLAPEGCVIKHTACPKEMFEATLLARPFNSEEECIDAVL
                     KRKVKPGDAIFIRYEGPKGSGMPEMFYTGEAICSDPKLASSVALITDGRFSGASRGPV
                     IGHVSPEAAAGGPIALVEEDDLIYINVKERRLEIVGVKGEKRTPEEMEEILKVRKAKW
                     QPKPPKYTKGTLKLFSEHAVSPIKGGYMM"
     gene            4393..4764
                     /locus_tag="ACZPCL_08525"
     CDS             4393..4764
                     /locus_tag="ACZPCL_08525"
                     /inference="COORDINATES: protein motif:HMM:NF016826.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HIR1162546.1"
                     /translation="MHGKTSATAGDICFSWTTNGGNGAWTSWPPHEHEKDLEEVYCYF
                     DMDDPHFGLHLSYVEPGDTHGVVAHTVKSGTMILAPRGYHPTVAAPGTRNTYLWVLAA
                     HSHKSRRYDLAVLDPKRAEMN"
     gene            4811..5893
                     /locus_tag="ACZPCL_08530"
     CDS             4811..5893
                     /locus_tag="ACZPCL_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR1162547.1"
                     /translation="MNAMDSIFLPRFTIGENAFDAFHDEMGRYGKKVAVIHGERAWKA
                     AREYVVPALEKAGLTITTEWLYGHDATHENADKISNDPAVQEADMLLAVGGGKCIDTA
                     KLAADHLGKPVFTAPSIASNCAPVTQISIMYHEDGSFCDIPRLKEVPVHCFINPKLVL
                     AAPVKYLWAGIGDAMAKHVESSWSAKAGEKLSFGSEFGITAGQMCFYPMVKDAKKAMD
                     DAKAGRNSEELENTILNIVVSPGVVSVSVHPNYNGGIAHALFYGLTKREHIEKKHLHG
                     EVVSYGTLVNLMVDKDWDKLKLAYGVNKSIDLPVCLADLELEKDDKLEDVLEATMANQ
                     EMTHTPYPVTKEMIYQAIQDLEDYKG"
     gene            5927..7174
                     /locus_tag="ACZPCL_08535"
     CDS             5927..7174
                     /locus_tag="ACZPCL_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HIR1162548.1"
                     /translation="MAVTIRDIRVICTAPEGINLVVVKVETSEPGLYGLGCGTFAYRH
                     LAVKLVVEEYLKPLLVGRNVENIEDLWQLMNQNGYWRNGPIENNAISGVDMALWDIKG
                     KMANMPLYQLFGGKCREGIPVYRHADGRDLSELCENIERFHEMGITNIRCQCGGYGGE
                     SYGNTPATAPKGAQPGVYLDSRSYIRNTVKLFEGIRNKLGYEINLVHDVHERIAPIEA
                     IKLARELEPFDLLFLEDPVPIEQTDWLARMREQTSIPIAEGELFNNPVEWRKLISERL
                     IDYIRVHLSQVGGITPARKLQIFAEQFGVRTAWHGPGDMSPLAHAANIHIDLAAPNFG
                     VQEWSGIEPPNFVIQELKGPHGALLEVFPGLPEFDNGYVYANDKPGLGVDLNEEEAAK
                     YPCENTVTTWTQTRRRDGALQTP"
     gene            7305..8084
                     /locus_tag="ACZPCL_08540"
     CDS             7305..8084
                     /locus_tag="ACZPCL_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1162549.1"
                     /translation="MAFDYKEKFSVAGKKCIVTGAAQGLSRGMAEGLLENGAEVVLMD
                     LQAEKLQAVADEYCKMGYKAHAVAGDLSKKPEIDRMFAEAMEILGNKLDVMIPAAGIQ
                     RRYEPWEFPEDAWRLVIDVDLNHVWFMCQKAVQVMKDKEGSTGKIINIASMNAFFGGT
                     TVPAYTAAKGAVAMLTKSLASDCADHNICCNAIAPGYMDTEMCANMTQERKDECTKRI
                     PAARWGLPDDLKGPVLFLASDASDYLNGAVIPVDGGFLVKS"
     gene            8300..9034
                     /locus_tag="ACZPCL_08545"
     CDS             8300..9034
                     /locus_tag="ACZPCL_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR1162550.1"
                     /translation="MRQTQKEKLLPEQTAEKIREYIEGNGLKVGDKLPNEFQLAEICG
                     VGRSTVREAIKLLVFSGKVEVIRGSGTYIKKEKVSQASPAVNDDPIGLQENSEENLFQ
                     TGLEFVDVRLMLEPEVAAMAATNADYADCHKLQQIQKKVEDLVAQGKDHIAADIEYHR
                     QLAACTHNQTLCNLMEIVVKGIPIFVQMTKNNFAKTTVDQHQAITDAISSADAPGARY
                     AMITHLNSNRKRILKVLTEQKSNGKL"
     gene            9124..>9762
                     /locus_tag="ACZPCL_08550"
     CDS             9124..>9762
                     /locus_tag="ACZPCL_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR1162551.1"
                     /translation="MEKSFLTWTAAALGTAILLTACNTNTGTTAPGSKVKLDPSKPVS
                     LTVWHYYNGAQQAAFDQLVSEFNATEGKDKGIYVEGYTQGSVSDLEKAVSDAVSGAVG
                     AQSLPDIFSTYADTAYAVQKKDKLADLSSYFTEEELSEYVDSYIKEGYFHDDTSLYLF
                     PVAKSTEIMMINKTDWKPFADATGSSLEELSTLEGVADVAKRYYEWTDSLTPD"
BASE COUNT         3021 a         1882 c         2478 g         2381 t
ORIGIN      
        1 tggagtacat aggaaacgcc agctgccaca gcaccggaaa tagcggcaaa ggctacaaac
       61 tttccaaaac ctttcttagc catacaaata cccccttctt aaaatagtgt ctgaatttat
      121 aaattagtat gtctgattat tatattgtaa tacgaacaga aaaaaaaaga aactaaaatt
      181 gcaaaaaaat ataaaagatt tacaaagtta agagacaaaa tgaaaaaatt agcactcaga
      241 acttgacagt gctaacaata ggtgctataa cttacattgt gagcgaaaga agcagcgaaa
      301 agtaaaagct gcaggtagaa aaataagaat gacgatcaag gaggaatcta gttatgaagt
      361 tagttccatt atttgacaaa gttgtattaa agcagttagt tgcagaagag accacagcat
      421 ccggtattgt tcttccaggt gcagcaaaag aaaaaccaca gcaggcagaa gttatcgcag
      481 taggcccagg cggtgtagtt gacggcaagg aagttaccat gcaggttaag gctggagata
      541 aagttatcta ctccaagtac tcaggtacag aagtagagtt agagaaagaa aaatacgtga
      601 tcgtaaagca aagcgacatt ttagctgttg tagagtaaaa aatgccagag gcatttttcg
      661 ctacgcaaac gcagcgcagc ggagttcgcg tttcccgtat agtaggtata aatacgaaat
      721 agtttagatt gttcggaggt aaataatcat ggcaaagcag atcaaatatg gtgtagaagc
      781 ccgcaaggca ctggaagcag gcgtaaatca gttagcagat acagttcgtg taaccttagg
      841 accgaaagga cgtaacgtag ttctggataa gtctttcggt gctccactga tcacaaatga
      901 tggtgttacc attgcaaaag agatcgaact ggcagatcca tttgagaaca tgggcgcaca
      961 gctgatcaag gaggttgctt ccaagaccaa tgatgtagct ggtgatggta ctactaccgc
     1021 aactgtactg gcacaggcta tggtaaatga aggtatgaag aacctggctg caggtgctaa
     1081 cccaattatc ttaagaaaag gtatgaagaa agcaactgat gcagctgtag aagcgatcaa
     1141 gaagatgagc cagccagtag gcggcaaggc acagatcgca agagtagcag ctatctctgc
     1201 ttctgatgat gaagtaggaa ccatggtagc agatgctatg gaaaaggttt ccaaagacgg
     1261 cgttatcacc atcgaggaat ccaagacaat gaagacagag cttgacctgg tagaaggtat
     1321 gcagtttgac agaggttacc tgtccgcata tatgtgcacc gatatggata agatggaagc
     1381 aaatctggat gatccataca tcttaattac tgataagaag atctccaaca tccaggagat
     1441 cctgccaatt cttgagcaga tcgtaaagat gggcgcaaga ctgctgatca tcgctgaaga
     1501 tgtagaaggc gaagctctta caaccctgat cgtaaacaag ttaagaggta ctttcaatgt
     1561 agttgctgtt aaggctcctg gctacggcga cagaagaaaa gaaatgctgc aggatatcgc
     1621 tatcttaacc ggtggtactg ttatctccga agaagttggt ctggaattaa aggatgctac
     1681 catggagcag ttaggccgtg caaaatccgt taaggttcag aaagagaaca cagtgatcgt
     1741 tgatggtaac ggcagcaagg aaatgatcgc agcaagagtt gcccagatcc gtaagcagat
     1801 cggtgagacc acttctgatt tcgacagaga gaaattacag gagcgtctgg ctaagttagc
     1861 tggcggtgta gctgttatcc gtgtaggtgc tgctactgag accgagatga aggaagctaa
     1921 gcttcgtatg gaagatgctt taaatgcaac ccgtgcagca gttgaagagg gaattatcgc
     1981 aggcggcggt tctgcttatg cacatgctac cgttgatgtt cagaaggtag ttgatactct
     2041 ggaaggtgac gagaagaccg gtgccaagat catcttaaag gctctggaag ctcctctgtt
     2101 ccacattgta gcaaacgcag gtctggaagg cgctgttatc gtaaacaagg taaaagagtc
     2161 cccggttggc gaaggctttg atgcttacaa tgagactttc gtaaacatga tcgaagcagg
     2221 tatcctggat cctgttaagg ttacaagaag tgcattacag aatgcaaaca gcgttgcatc
     2281 tacattactg accactgagt ctgtagtagc aaccatcaag gagccagctc cggctgctcc
     2341 ggctgcacct gatatgggcg gaatgtatta atcagaaata taaaaatagg aatatactgt
     2401 tagtaaaaag aagtctgtgg cggataaaaa cgttgcagac ttcttttttt gttaaaaatt
     2461 tgtctgacct attgcctttt taataaaaaa atgctaatct aaactcatga gatgtcagac
     2521 gaatttttaa aaaataatat gatgaattaa aaggagaacg gttatgaagt tacacagtca
     2581 ggaagttaga aaacttgcac cggaaaatga cccgttaaag atcggaatgg gatggagcgt
     2641 ggcagatctg gataaacctc agattttagt agaaagctct tttggtgaca gtcatccggg
     2701 aagtgcccat ctgaaccagt ttgtagaaga gggcgtaaaa gcagtcaacg ataacggagg
     2761 aaaagcagcc agatattatg tgaccgatat ctgcgacggc attgcccagg gacatgacgg
     2821 catcaattat tctcttgcac acagagatat gatgaccaat atgatcgaag ttcacggaat
     2881 ggcaagcggc tttgacggcg gcatgtttat tgccagctgc gacaagtccg tgcctgctat
     2941 tttaatgggt atcggccgtt taaaggatat gagtgctatt gtagttactg gcggtgttat
     3001 ggaggcacat actattacac ctaagtatgt ggaaaatgac ccggcctgcg ctattaacga
     3061 actgctgaca ctggagcaga tcggtaaatt cgatgcacag gaaaagagag gcgagatccc
     3121 ggaggaacag ttaaattatt ataagcacaa tgcatgccca agctgcggcg catgttcctt
     3181 tatgggtact gcttccacca tgcagatcat ggcagaggca ttaggcctta tgcttccagg
     3241 cactgccctg atgcccgcaa ctgcacctga attaaagcag gctgcttatg atgcaggaaa
     3301 acagctgatg aagttagtag aaaaaggtat caaggcaaag gatatcgtta ccatgaagag
     3361 ctttgagaac gctattatgg tacatgctgc tatcagcggt tctaccaatg ctaccatgca
     3421 cattcctgct attgcccatg aattcggctt tgaagtagat gcagaaacct ttgaccgtat
     3481 gcacagagga gctcattacc tgcttaatat ccgtccatct ggtgactggc cagcccagta
     3541 tttctattat gcaggcggtg ttccaagagt tatggaagag attaaggata tgctgcatct
     3601 ggatgtaatg acagttacag gcaagacttt aggtgagaac ctggaagaat taaagaaaaa
     3661 tggtttctat gaccactgcg atgagctgtt aagggaaaag ggcaagctgt taggacgcga
     3721 gatcaagcgt acagagatca tccatgcctt tgatgatgca aagggaacag atggaagtat
     3781 tgctatttta aagggcaacc tggctccgga aggctgtgtg atcaagcaca ctgcatgccc
     3841 gaaggaaatg tttgaggcca cccttcttgc aagaccattt aacagtgagg aagagtgtat
     3901 tgacgctgtt cttaagagaa aggtaaaacc aggggatgct atctttatcc gctatgaggg
     3961 accaaaggga agcggtatgc ctgaaatgtt ctatacaggt gaagctatat gttctgaccc
     4021 gaaactggct tccagcgtgg ctctgatcac tgacggacgt ttctccggtg catccagagg
     4081 cccggttatc ggtcatgtaa gcccggaagc tgctgcaggc ggacctattg cactggtaga
     4141 agaagatgac ttgatctata ttaatgtaaa agagcgcaga ctggagatcg taggcgtaaa
     4201 gggtgaaaag cgcactccag aggaaatgga agagatatta aaggtcagaa aagctaaatg
     4261 gcagccaaag cctccgaaat ataccaaggg aacccttaag ctgttctctg aacatgcagt
     4321 atcacctatt aaaggtggat atatgatgta gtccaaagaa atcttcgaat gcgtcactgg
     4381 cgcattctcg atatgcatgg caaaacaagc gccaccgcag gtgacatttg tttttcttgg
     4441 accaccaacg gcggaaatgg tgcatggacc agctggccgc ctcatgagca tgaaaaggat
     4501 ctggaagagg tttattgcta ctttgatatg gatgatcctc attttggact gcatttaagc
     4561 tatgtagaac caggagatac ccatggagta gtagctcata ctgttaagag tggtactatg
     4621 atcctggcac caagaggtta ccatcctaca gtagctgcac cgggaaccag aaatacatat
     4681 ttatgggtac tggcagcaca ttcccacaag agtcgtcgtt atgatctggc agttctggac
     4741 ccgaagcgcg cagaaatgaa ctaggccaga aataaagtaa aattaagagt tatatttagg
     4801 aggcagagac atgaacgcaa tggattcaat ttttctccca cgttttacca ttggcgagaa
     4861 tgcatttgac gctttccatg atgaaatggg aagatatgga aaaaaagtag cagtgatcca
     4921 cggagaaaga gcatggaagg cagcaaggga atatgtagtt cctgcactgg aaaaagcagg
     4981 tcttacgatt actacagaat ggctgtatgg gcatgatgca acccatgaaa acgctgataa
     5041 gatctcaaat gatcctgcag tacaggaagc agatatgctc cttgcagtag gcggtggaaa
     5101 atgtatcgat acagcaaagc tggctgcaga tcaccttgga aaaccggtat ttaccgctcc
     5161 ctccattgcg tctaactgtg ctcctgttac ccagatcagt attatgtacc atgaggatgg
     5221 ttctttctgc gacatcccaa gattaaagga agtaccggta cactgcttta tcaacccgaa
     5281 actggtttta gcagctcctg ttaaatacct gtgggctggt atcggcgatg ccatggcaaa
     5341 gcatgtagag tccagctggt ctgcaaaggc tggggagaag ttaagctttg gcagtgagtt
     5401 tggtatcaca gcaggacaga tgtgcttcta tccaatggtt aaggatgcga agaaggcaat
     5461 ggatgatgca aaagcaggaa gaaacagtga agaacttgaa aacaccattt taaatattgt
     5521 tgtttcccca ggtgttgtat ctgtttctgt tcatccaaat tacaatggcg gtattgcaca
     5581 tgctttattc tatggcctga ccaagagaga gcatattgaa aagaaacatc tccacggtga
     5641 ggtagtttcc tacggcacac tggtaaatct tatggtagat aaggactggg ataagctgaa
     5701 actggcttat ggcgtaaata agtctattga cctgcctgta tgcctggctg atctggagct
     5761 ggaaaaggat gacaagttag aggatgtcct tgaagcaaca atggcaaacc aggaaatgac
     5821 ccatacacct tatcctgtaa caaaagagat gatctatcag gcaatccagg atctggaaga
     5881 ctataagggt taaaaaccta gaatgtaagt tagagaggat agtaaaatgg cagtaaccat
     5941 acgtgatata cgggtaatct gcacagcgcc ggaaggcatc aaccttgtgg tggtaaaagt
     6001 tgagaccagc gagccgggac tttacggact gggctgcgga acctttgctt accgccacct
     6061 ggctgtaaag ctggtagtag aagaatattt gaagcctctt cttgttggaa gaaatgtaga
     6121 gaatatcgaa gatttgtggc agttgatgaa ccagaacgga tactggagaa acggccctat
     6181 tgaaaataat gctatttccg gtgtagatat ggcattatgg gatattaagg gaaaaatggc
     6241 aaatatgccg ctgtaccagc tgtttggcgg aaagtgcagg gaaggtattc cggtataccg
     6301 ccacgcagat ggccgtgatc tttccgagct gtgtgaaaat attgagcgtt ttcatgaaat
     6361 gggtattacc aatatccgct gtcagtgcgg cggttacggc ggcgaaagct atggaaatac
     6421 gccggcaact gcaccaaagg gcgcccagcc aggtgtttat ctggattcca gaagctatat
     6481 ccgcaataca gtaaagctgt ttgaaggcat cagaaacaaa ttaggatatg agatcaatct
     6541 ggtacatgat gttcatgagc gtattgctcc tattgaggct attaagctgg caagagagct
     6601 ggagccattt gatctgttat tcttagagga tccggtaccg atcgagcaga cagactggtt
     6661 agcaagaatg agagagcaga cctccatacc aatcgcagaa ggcgagctgt tcaacaatcc
     6721 tgttgagtgg agaaaactta tctcagaacg gctgatcgat tacatccgcg tacatttaag
     6781 tcaggttggc ggtattacac cggcaagaaa gctgcagatc tttgcagaac agtttggtgt
     6841 acgcactgca tggcacggac ctggagacat gtctcctctg gctcatgcag caaacatcca
     6901 tattgacctg gcagcaccaa actttggtgt acaggaatgg tccggcattg agcctccaaa
     6961 ctttgttatc caggaattaa agggaccaca tggtgctctc cttgaagtat tcccgggact
     7021 gcctgagttt gacaatggtt atgtatatgc aaatgataaa cctggtttag gcgttgactt
     7081 aaatgaggaa gaagctgcaa aatatccttg tgagaatact gtgactacat ggacacagac
     7141 aagacgcagg gacggagcat tgcagactcc ataaagggat gaaggatccc cgggttctta
     7201 caccaatctc cccggtttac cggggaaaat caaaatatat ttatacattc cgctcccgta
     7261 tcacgggagc gggccaccat atattattat aggaagataa aaaaatggca tttgattaca
     7321 aggaaaaatt cagcgtagct ggcaaaaagt gcatcgttac cggtgcagca cagggacttt
     7381 ccagaggtat ggcagaaggt ctattagaga atggcgctga ggttgtatta atggaccttc
     7441 aggcagaaaa gctgcaggca gttgcagatg aatactgcaa gatgggctac aaggcacatg
     7501 cagtagcagg tgacttatcc aagaagccag agatcgaccg tatgttcgca gaagctatgg
     7561 agatcttagg aaataagctg gatgttatga tcccggcagc aggtatccag agacgttacg
     7621 agccatggga attcccggag gatgcatgga gactggttat tgatgttgac ttaaaccatg
     7681 tatggtttat gtgccagaag gcagttcagg ttatgaaaga taaggaaggc agcaccggaa
     7741 agatcatcaa catcgcttct atgaacgcat tctttggcgg tacaactgtt cctgcttaca
     7801 cagcagctaa gggtgctgta gcaatgctga ccaagagcct tgcatctgac tgtgctgacc
     7861 acaatatctg ctgcaacgca attgctcctg gatatatgga tactgaaatg tgcgctaaca
     7921 tgactcagga aagaaaagac gagtgcacca agagaatccc tgcagcacgt tggggactgc
     7981 cagatgacct taagggaccg gttctgttcc tggcttctga tgcaagtgac tacttaaatg
     8041 gtgctgttat ccctgtagac ggcggattct tagttaagtc atgatgatgt cctcacaggt
     8101 gggatagtta aatagtacac ttagaaaagg ggtgctgtag taagacgatc agtcgggctg
     8161 cagtgcccct tttcaaaatc ttccattttt gaaacaccca gagggtgtta ggggcttgac
     8221 attcatggtt cgttagttat atcatgaaat aacgatggat aaacgtcagg tatttttgaa
     8281 ataattggga ggtggtcgcg tgcgacagac gcaaaaagag aaactgctgc cggagcagac
     8341 agcagagaag ataagggaat acatagaagg taatggatta aaggtaggag ataagcttcc
     8401 aaacgaattt cagcttgctg agatctgtgg agtaggaaga agtaccgtgc gggaagcgat
     8461 caagcttctg gttttttccg gaaaagtaga agtaatccgt ggaagcggta cttatattaa
     8521 gaaagaaaaa gtttcccagg catcaccggc ggtaaatgat gatcctatag gacttcagga
     8581 aaacagtgaa gaaaatctgt ttcagacagg tctggaattt gtagatgtac gtctgatgtt
     8641 agagccggaa gtggcagcta tggcagctac caatgcagat tatgctgatt gtcataaact
     8701 tcagcagatc cagaagaagg tagaagacct ggttgcccaa ggaaaagatc atattgctgc
     8761 ggatattgag taccacaggc agttggcagc ctgcacccat aaccagaccc tttgcaatct
     8821 tatggagatc gtggtaaagg gaattccgat ttttgtacag atgacaaaaa ataattttgc
     8881 caaaacaaca gtggaccagc accaggcgat cacagatgcc atttcatctg cagatgcccc
     8941 gggagcaaga tatgcaatga tcacccactt aaacagcaac cgcaaacgga tcttaaaagt
     9001 gctgacagag caaaaatcaa atggaaagtt ataatcccct gtgttttcat tgaaagttat
     9061 tgtcaatttc gttataatag tgttaaaaaa cagattgtac atgatcggtt cgggggacac
     9121 attatggaaa aaagtttttt gacatggaca gcagccgctt taggaacggc aattctttta
     9181 acagcatgca atacgaatac aggaacaact gcgccaggca gcaaggtgaa actggaccca
     9241 agtaaaccgg tttcccttac tgtctggcat tattataatg gggcacaaca ggctgccttt
     9301 gaccagctgg tttctgaatt taacgctaca gagggaaaag acaagggtat ttatgtagaa
     9361 ggatataccc agggatctgt atctgatctg gaaaaggcag tttcagatgc agtatccggt
     9421 gctgtaggtg cccagtcttt gccggatatt ttctctacct acgcagatac tgcctatgca
     9481 gtacagaaaa aggataaact ggcggatctg tcttcttatt ttacagagga agaactgtct
     9541 gaatatgtgg actcttacat aaaagaaggc tatttccatg atgatacctc tctttatctc
     9601 ttccctgtag caaagtctac agagatcatg atgatcaata aaacagactg gaagccattt
     9661 gcagatgcta ccggttcttc tttagaggaa ctttctactt tagagggagt ggcagacgtt
     9721 gcaaagcgtt actatgagtg gaccgacagt ttgaccccgg at
//
LOCUS       DBMDUA010000102         9746 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3327_length_9746_cov_2.648834, whole genome shotgun sequence.
ACCESSION   DBMDUA010000102 DBMDUA010000000
VERSION     DBMDUA010000102.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9746)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9746)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9746
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3327_length_9746_cov_2.648834"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1655)
                     /locus_tag="ACZPCL_08555"
     CDS             complement(<1..1655)
                     /locus_tag="ACZPCL_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR1162552.1"
                     /translation="MNENKDYVSHLNKARKKRNQKWDPRYLLLAIVVVLLIGLGVLAA
                     VNVKSAVSGKAARKSDNVIEEPGTGLEAASASNAAREEEEKAKEEQEIQSAIDAYQNL
                     GIVQVSGYVNIRETPDMKGNIIGKVSGDGACEVLGEEGEWSHITSGGIEGYISSQYLV
                     TGEEAKELAKSLVKKRAIIMTENDNLNIRSGPSKDAEIVGQALPAERYEVLSEADGWV
                     EINSGYISADYCEVKYALNEGRKLDLKAQAINQYDNLVIFKKSGYMNVRSTPENKGDD
                     NVIGKLTSKAAGDIIETLDGWYKIKSGTVTGYIAADPELIATGQEAKDLAMQNATQMA
                     IITTDVLNVRVEPNTDSKIWTQIVKDERYPVVDQQDGWVQIDLGSVDAEDGSQDGDEK
                     AYISTRDNNVEVRYALNEAIKFTPAKDSSSGASSDGSGSSTKQSRRSQLVNYALQFVG
                     NRYVWGGTSLTNGVDCSGFTMRVMEKFGVSLPHYSGSQAQMGKKVTSATMKPGDLIFY
                     AGSNGKVNHVAIYIGNGRIVHAASRRSGIKTSTWNYRTPVAIR"
     gene            complement(1904..2701)
                     /locus_tag="ACZPCL_08560"
     CDS             complement(1904..2701)
                     /locus_tag="ACZPCL_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1162553.1"
                     /translation="MLQIKHVSKTFNKGTVNEKQALRDLNLTLKEGDFVTIIGGNGAG
                     KSTMLNAIAGVWPVDEGQIIIDDKDVTKLPEYKRAAFLGRVFQDPMNGTAATMGIEEN
                     LALALRRGTLRTLKPGINNSERKLYRDLLLRLGLGLEDRLTSKVGLLSGGQRQALTLL
                     MSTLKRPKLLLLDEHTAALDPKTARKVLELTEEMVNEQHLTALMVTHNMKDAIQIGNR
                     LIMMNSGHIIYDVEGEEKKKLTVENLLAKFSEASGEEFSNDRMMLAK"
     gene            complement(2701..3618)
                     /locus_tag="ACZPCL_08565"
     CDS             complement(2701..3618)
                     /locus_tag="ACZPCL_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1162554.1"
                     /translation="MNIMSLLNALPGAVAQGLIWGIMAIGVYITFRILDFADLTVDGS
                     LCTGGAVCIMMMLNGQNVWVAMFAALLAGMVAGCVTGLLHTFMGIPAILSGILTQLGL
                     YSINLKIMGKANQAINVDKYSLLVSLRFIRNVPLFKNTILLVSLIIVVLIVILYWFFG
                     TELGCSIRATGCNDKMARAQGINTDFNRVLGLMISNGMVALSGALLSQYQGFADINMG
                     RGAIVIGLAAVIIGEAIFGKIFRNFGLRLLSVAFGSIIYYLVLQIVIWLGIDTDLLKL
                     LSAAVVAIFLAIPTWKARYFSMGSKGGKA"
     gene            complement(3730..4782)
                     /locus_tag="ACZPCL_08570"
     CDS             complement(3730..4782)
                     /locus_tag="ACZPCL_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1162555.1"
                     /translation="MKKSTKIMSVLLAAACAASMTGCGGSGSTATTAAASEAATEAAS
                     SEEAKSDAAASTDGKTYNVGICQLVQHVALDAATQGFKDALTDALGDAVTFDEQNAQG
                     DSNTCSTIINSFVSNHVDLILANATPSLQAAQAGTNEIPVLGTAVTEYGVALGIDGFD
                     GLVGGNISGTSDLAPLDEQAAMLHELFPDAKNVGLLYCSAEANSQYQVDTVKAALEEM
                     GYTCEYYAFSDSNDLATITTNAATNSDVIYVPTDNTVAANTEIVNNICQPAKVPVIAG
                     EEGICSGCGVATLSISYYDLGVATGKMAVKILTGEANVSEMPIEYAPQFTKKYNKTIC
                     DALEITVPDDYVAIEE"
     gene            complement(4975..6387)
                     /locus_tag="ACZPCL_08575"
     CDS             complement(4975..6387)
                     /locus_tag="ACZPCL_08575"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1162556.1"
                     /translation="MRKAILEKFIFVLLAALCINSVIFYIASRNTILDTTKKDMLYTM
                     EILDQTLDYEGDLAGQVQRMENFTAGNPSRLTLIRTDGTVVSDSDADPAELDNHLSRK
                     EVVQAMKKGSGFAERYSHTLKRNLLYVAYRSNRADMIIRVSVPYSGASEYLTMLLPAA
                     LFSFLAALACALVVSKRFVDSVTQPLSNIAAEMSKVKGDYKGDYTELHFEKCQYPELN
                     VIAETTMEMSKNVKAYLSRIDKEKQIRQEFFSNASHELKTPITSIQGYAELMESGMIQ
                     DENMKMDFIKRIKREAINMAGLINDILMISRLEAKDAEVVVSDVRVSVLLEEIIDSLK
                     PAAAAGQVFIHCDCQPLSIKANPQQMKELLSNLVSNAVKYNRPGGQVWVNIREKDGNM
                     VARVRDNGVGIPQESLDRIFERFYRVDKGRSRKQGGTGLGLSIVKHIVNFYHGTIRVT
                     SQLDEGTEFVVTIPLNWKDL"
     gene            complement(6410..7096)
                     /locus_tag="ACZPCL_08580"
     CDS             complement(6410..7096)
                     /locus_tag="ACZPCL_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1162557.1"
                     /translation="MDKIYIIEDDENIRNLLKIALEGFGYEAEGFETAEEGLERIKEE
                     VPSLAIFDWMLPGMDGVTAIRKIRQTDGLQELPIMLLTAKEKELDKVVGLDCGADDYM
                     VKPFGVLELSARIRSLLRRAKREEKKDTLTFEDIVVNKKTREVTSEGHLCELTLKEFE
                     LLVYLMENQSRVVTRDELLNQVWGYEYDGETRTLDMHIRTLRQKLGDKESSCIKTVRG
                     VGYRLVHQEG"
     gene            complement(7275..8453)
                     /locus_tag="ACZPCL_08585"
     CDS             complement(7275..8453)
                     /locus_tag="ACZPCL_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1162558.1"
                     /translation="MLQSFKLAIKSIWGNKMRSFLTMLGIIIGVAAVIILVSLVNGYM
                     GSVVESFASMGVNQINVNMTNLTSRTVDADQMYAFYDEHGDLFDGISPNVSLSATVKK
                     GDDSMDSTSVAGRSEQYLDMKDYDLQIGRNIAYSDIASRQKVCVIGSYVAQELFGSAE
                     KAINETLKINGYAFKVVGIVETQDEDNMEDGGTDDFVWIPYSVATSLAGSANISSYTL
                     TVSDTDNADTAKTLIQNFLYETFKDSDLYRVTAMSEMLDSLNEQIAMMSGMLGGIAGI
                     SLLVAGVGVMNIMLVSVTERTREIGIRKSLGADKGTILQQFVIEAAVTSSLGGVVGII
                     IGCVATKVVGTMIGISTSPTLAAVLISFGVSVAIGLFFGYMPARRAANLNPIDALRSE
                     "
     gene            complement(8437..9270)
                     /locus_tag="ACZPCL_08590"
     CDS             complement(8437..9270)
                     /locus_tag="ACZPCL_08590"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1162559.1"
                     /translation="MFRLWKGRKDAENKTNNLNEKNTHEIKSQIENDGTNGTDLDKPG
                     SHLIDLRHIFKIYYLGGEEVRANDDVSVAIDKGEFVAIVGKSGSGKSTLMNIIGALDV
                     PTKGEYYLGGEDVSKMEDKQLARIRNKMIGFIFQQYNLLPKLNLLENVELPLLYAGVG
                     SEERRQRAMASLEKVGLAEKWKNMPNQLSGGQQQRVSIARALAGDPSLILADEPTGAL
                     DSKTSREVLNFLKQLNEEGNTIVMITHDNSIAMEAKRVVRIADGHKIFDGDVKDYAAI
                     L"
     gene            complement(9312..>9746)
                     /locus_tag="ACZPCL_08595"
     CDS             complement(9312..>9746)
                     /locus_tag="ACZPCL_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HIR1162560.1"
                     /translation="SNGVSTYPVTVTMDEVGDLLPGMNVDGVITLEEANDVLTIPVDA
                     LMRGNQVYIKDDSVKEQQGPVPAGFKAVEVETGLTNDSYVEIKSGLSEGDTVYVAKSS
                     TGSTNSFMGGPGMGGPGGGMGGPPSGGGNDGGNRGGNGGGPR"
BASE COUNT         2419 a         2462 c         1964 g         2901 t
ORIGIN      
        1 ctgcggatcg ccaccggtgt acggtaattc catgtagatg tcttaatacc gcttctgcgg
       61 ctggctgcat gtacgatacg gccatttcca atgtaaatcg ctacatggtt tacttttccg
      121 ttgcttcctg catagaagat cagatctccc ggtttcatag tagcggaggt gactttctta
      181 cccatctgtg cctgggagcc ggaataatgt ggaagggata caccaaattt ctccataacc
      241 ctcatagtaa atccggagca gtcaacacca ttggtcagac tggttccgcc ccatacatag
      301 cggtttccta caaactgaag agcgtagttt acaagctgtg aacggcggga ctgcttggta
      361 ctggagccgg agccgtcaga tgaagcaccg gaactgctat ccttggccgg tgtaaattta
      421 atggcttcat ttaaagcata acgtacttct acgttattgt cgcgggttga aatatatgct
      481 ttttcatctc cgtcctggct gccatcctct gcatccacag aacctaagtc gatctgtacc
      541 cagccatcct gctggtctac taccgggtaa cgctcatctt tgacgatctg tgtccagatc
      601 ttggagtcgg tatttggttc cacacggacg tttaatacat ccgttgtgat aatagccatc
      661 tgggtagcat tctgcattgc cagatctttt gcttcctgtc ctgttgcgat cagttccgga
      721 tctgctgcaa tataaccggt tactgtgccg gacttgatct tataccagcc atctaacgtc
      781 tcaataatat caccggctgc cttactggtc agcttaccaa ttacattgtc atcaccttta
      841 ttttccggag tgctgcgcac attcatatag ccgctctttt taaagatgac cagattatca
      901 tactggttaa tggcctgtgc tttcagatcc agtttacggc cctcatttaa ggcatatttt
      961 acttcacagt aatcagcgga aatataacca ctgttgatct ctacccagcc atctgcttca
     1021 gaaagtactt cataacgctc tgcaggaagt gcctgtccta cgatctctgc atccttgctt
     1081 gggccgcttc ggatattcag attgtcattt tctgtcatga taatggcacg tttcttcaca
     1141 agactttttg ccagttcttt tgcttcctct cctgttacca ggtactggct gctgatatag
     1201 ccttcaatac cgccggaagt gatatggctc cattcgccct cttctcctaa aacctcacag
     1261 gctccgtctc ctgaaacttt tccgataata tttcccttca tatccggagt ttcacggata
     1321 tttacataac cggatacctg aacgatccct aaattctggt atgcgtcaat ggcgctctgg
     1381 atttcctgtt cttcttttgc cttctcttct tcctctcttg ctgcattgga agcgctggct
     1441 gcctcaagac ccgttccagg ctcctcgatc acattatcac tttttctcgc tgccttgcct
     1501 gaaaccgccg actttacatt taccgccgct aatactccaa gaccaatgag cagtacaacg
     1561 acaatggcta aaagcaggta acggggatcc catttctggt ttctcttttt tctcgcctta
     1621 ttcaggtgag atacgtaatc cttattttca ttcattgttt tgtttctcct gttattacta
     1681 gagcatctgc aggacctgcc tgcggcagtt tcttacagat gcatggcaat ttatagacac
     1741 gcctcctggc gcactatatg taagtatatc atatatttcg ctgcaaaaag tgaacaaagt
     1801 attaaaaatg ttaaaaatta ttgacatgca tagaaaaaaa gattgcccga aaaggctata
     1861 taccatagta tacattcccc tccgggcatt ggatcacatt tttctatttt gccagcatca
     1921 tacggtcatt ggagaattct tctccgcttg cttcggagaa ttttgctaac agattttcta
     1981 ctgtcagttt ctttttctct tctccttcca catcataaat aatgtggccg ctgttcatca
     2041 tgatcagacg gtttccgatc tggatggcat ctttcatatt gtgggtaacc attaaagctg
     2101 tcaggtgctg ttcgttgacc atttcctctg tcagctccag aacttttctt gcagtcttcg
     2161 ggtcaagagc tgccgtatgc tcatctaata aaagcagttt cggccgcttc aaagtagaca
     2221 taagcaatgt cagagcctgt ctctgtccgc cggagagaag tcccactttg gaagtcagac
     2281 ggtcttccag tcccagtccc agtcttaaca gcagatctct gtaaagcttt ctttcactat
     2341 tattgatacc cggttttaag gtacgaagag ttcctcttct taaagccaga gccagatttt
     2401 cctcaattcc catcgtagcg gcagttccgt tcataggatc ctggaataca cggcctaaga
     2461 aagcggctct cttatattcc ggaagtttgg tcacatcttt atcatcaatg atgatctggc
     2521 cttcgtctac cggccacaca cctgcaatag cgtttaacat agtagacttg ccggcaccat
     2581 taccgccgat aatggtaacg aaatctccct ctttcagcgt aaggtttaag tcccttaacg
     2641 cctgtttttc atttactgtg cctttattaa aggtttttga tacatgtttg atctgaagca
     2701 tcaggcttta cctcctttag aacccattga aaaataacgt gccttccatg ttgggattgc
     2761 caggaagatg gctactactg ctgcagacag cagctttaac aggtctgtat cgattcccag
     2821 ccagatgact atctgaagaa ccagataata aataatggaa ccgaaagcaa cacttaaaag
     2881 gcgtaagcca aagtttctga agatctttcc gaaaattgct tctccgatga taacagcagc
     2941 cagaccgata acgatagcgc cacggcccat attgatatca gcaaagccct ggtactggga
     3001 aagaagagct ccggaaagag ctaccatacc gttagagatc atcagaccca gaacacgatt
     3061 aaagtcggtg ttaataccct gggctcttgc catcttgtca ttgcagccgg ttgcacggat
     3121 ggagcagcca agctcagtac cgaagaacca gtaaaggata acgatcagca caacaatgat
     3181 cagggatact aaaagaatgg tatttttaaa gagaggcaca ttgcggataa aacgtaaaga
     3241 aacaaggagg ctgtatttgt ctacgttgat cgcctggttt gcctttccca ttattttcag
     3301 gttaatagaa tacaggccta actgtgtaag aataccggaa aggatagccg ggatacccat
     3361 aaaagtatgt aaaagtccgg tgacgcagcc tgccaccatt cctgcaagga gtgctgcaaa
     3421 catggccacc catacattct gtccgtttaa catcatcata atgcagactg caccgccggt
     3481 acataaagaa ccgtctacgg tcagatccgc aaaatccagt atacgaaatg tgatataaac
     3541 accaatggcc atgatgcccc agatcaggcc ctgtgccact gctccaggaa gagcatttaa
     3601 taaactcatt atattcattg ttttcccctt tagaacagca tacagcggga ggcccatttg
     3661 ccttcccgct gtgtctgcca tgaaagtttt tttacattaa aagtttcttt gcaaacttag
     3721 tttttcttat tattcctcga ttgctacata atcatctgga actgtgatct ccagtgcatc
     3781 gcagatggtc ttgttgtact tctttgtaaa ctgaggtgca tattcgatcg gcatttcaga
     3841 aacatttgct tctcctgtaa ggatcttaac tgccatctta ccggttgcaa cacccaggtc
     3901 atagtagctg atggaaagag ttgcaacacc acagccggag cagatgccct cttctcctgc
     3961 gattacagga acctttgcag gctggcagat gttattaacg atctctgtgt ttgctgctac
     4021 tgtattatca gttggaacat agatcacatc gctgttggtt gctgcattgg tagtaatggt
     4081 tgcaaggtcg ttggagtcag agaatgcgta gtattcgcag gtatatccca tttcttccag
     4141 agctgccttt acagtatcta cctgatactg ggagttagct tctgcggagc agtataacaa
     4201 gccaacgttc tttgcatccg ggaacagttc gtgaagcata gctgcctgct catccaatgg
     4261 cgccaggtca gaagtaccgg aaatgtttcc gcctaccagg ccgtcaaaac cgtcaatgcc
     4321 aagtgctaca ccatactctg taacagcggt acctaataca gggatctcat tggttcctgc
     4381 ctgtgctgcc tggagagatg gggttgcgtt tgcaaggatc aggtctacat gattggaaac
     4441 aaagctgttg ataatggtag aacatgtgtt ggagtcaccc tgtgcgttct gctcatcaaa
     4501 ggtaactgca tcgcccagtg catcggttaa tgcatcctta aatccctggg tagctgcatc
     4561 cagtgctacg tgctgtacca gctggcagat acctacgttg taggtttttc catcggtgga
     4621 agctgctgca tcgctctttg cttcttcgga agaagctgcc tctgttgctg cttcagacgc
     4681 tgctgctgtg gtagctgtgc tgccggagcc accgcatccg gtcatagatg ctgcgcaggc
     4741 tgctgctaat aatactgaca tgatctttgt agattttttc ataataccgt cctcttttcg
     4801 ataaaaactc attcgtttta tacgaactaa gggtatgata ctacaaaaaa atccccacgt
     4861 caattttatt gcttaaaatt tcttagaatt taaagcaata aagtgttttt cgtcgttttt
     4921 accaatggat tttccagttt tttccagtta tatccttgac cggtcttaaa aattttacag
     4981 gtctttccag tttaaaggaa tagtcactac aaattctgta ccttcatcta actgggaagt
     5041 cacacggatg gttccatgat agaaattaac aatgtgcttt acaatggaaa gtccaagtcc
     5101 ggtaccgccc tgttttctgc tgcggccctt atccacccgg tagaaccgtt caaaaatgcg
     5161 gtccaggctt tcctgaggga tgccaacacc attatcccga acacgggcaa ccatattgcc
     5221 gtctttttcc cggatattga cccatacctg accacctggc cggttgtatt ttacagcatt
     5281 gctcaccaga ttggagagca gttctttcat ctgctgcgga ttggccttga tgctaagagg
     5341 ctggcagtca cagtgtataa atacctgtcc agctgctgct gcaggtttta aactgtctat
     5401 gatctcttca aggagcacac tgactctcac atctgataca accacttccg catcctttgc
     5461 ttccagtctg gagatcatca ggatatcatt aataaggccg gccatattga tggcttccct
     5521 tttgatcctt ttgataaagt ccattttcat gttctcatcc tggatcatac cgctttccat
     5581 taactctgca taaccctgga tagatgtgat aggagttttt aactcatggg aagcattgct
     5641 gaaaaactcc tgacggatct gtttttcctt gtcaatgcgg ctcagatagg ctttcacatt
     5701 tttggacatt tccatggtag tttcagcgat cacatttaac tccggatact ggcatttttc
     5761 aaaatgcagt tctgtatagt ctcctttgta atctcccttt acctttgaca tttccgctgc
     5821 aatattgctt aaaggctgtg ttacagagtc cacgaaacgt ttggaaacca ccagtgcaca
     5881 ggcaagagcc gccaggaagc tgaaaagagc cgccggaagg agcatggtca ggtattcgct
     5941 ggcaccggaa taaggcacgg aaacacggat gatcatatcg gcccggttgg atctgtaggc
     6001 cacatataaa agatttctct ttaatgtgtg ggaataacgt tccgcaaagc cactgccctt
     6061 tttcattgcc tggactactt cttttctgct taaatgattg tccagttcag ccggatctgc
     6121 atctgagtca gaaaccaccg ttccatctgt acggatcaaa gtcagacggc tgggatttcc
     6181 cgctgtaaaa ttctccatcc gctgcacctg acctgccaga tctccctcat aatccagagt
     6241 ctgatccaat atctccatgg tgtaaagcat gtcttttttt gttgtatcca gtatcgtatt
     6301 ccggctggca atataaaaga tgacgctgtt gatacacagc gccgctaaaa gtacaaagat
     6361 aaatttctca agaatggctt ttctcatact ttttcctctc tctccggttt tatccttcct
     6421 gatgcaccag tctgtaacct acaccacgga ctgtcttaat gcaggaactt tccttatctc
     6481 ccagtttctg acgcagagta cggatgtgca tatccagcgt tcttgtctcc ccgtcgtatt
     6541 catagcccca gacctgattt aaaagttcgt ctcttgtgac cacacgggac tggttttcca
     6601 tcagatagac caaaagttca aattctttta aggtcagttc acataaatga ccttcactgg
     6661 tcacttccct ggtctttttg ttgactacga tatcttcaaa agtaagggta tccttctttt
     6721 cttctctttt ggcgcggcgc agcaggctgc gtatccttgc agaaagctcc agaacaccaa
     6781 atggctttac catatagtca tcagcgccgc agtcaagacc tactactttg tccagctctt
     6841 tctctttggc agttaaaagc ataatgggaa gttcctgcag gccatctgtc tggcggatct
     6901 tgcggattgc cgtaacccca tccattcccg gaagcatcca gtcaaaaata gcaagtgacg
     6961 gtacttcttc tttgatgcgc tctaatcctt cttctgctgt ttcaaagcct tctgcctcat
     7021 aaccaaagcc ttccagcgct atttttaaaa gattgcgtat gttttcatca tcttcgatga
     7081 tataaatttt atccatggtg atcctcgtcc ttcccttttt aaccgcaaaa ccgcagtatg
     7141 cagttatgtt cttatcatca gcataccaca gctgtgtaaa aacaaaaaca gccaaatgta
     7201 aaatttgtgt aaaatctcac atttgactgt cagccgggtg gcgcttctcc ccctgctgca
     7261 ggagagaacg tttattactc acttctaagt gcatcaatag ggtttaagtt tgccgcccgt
     7321 cttgccggca tatatccaaa gaacaggcca atggctactg acaccccaaa ggagatcagc
     7381 actgccgcca gtgtagggga tgtggagatc ccgatcatgg ttcccactac ttttgtggct
     7441 acgcagccga tgatgatccc taccacgccg cctaaagaac tggtcactgc cgcctcaatg
     7501 acaaactgct gtaaaatagt tcccttgtca gctcccagtg actttctgat gccgatctct
     7561 ctggtacgct cagttacaga taccagcatg atattcataa caccaacacc tgcaactaaa
     7621 agggaaattc cggcaatacc acctagcata ccgctcatca tggcgatctg ttcatttaag
     7681 gagtccagca tctcactcat agctgttact ctgtaaaggt cactgtcttt aaaggtctca
     7741 tacaggaaat tctgaatcag ggtcttggct gtatctgcat tatctgtatc tgataccgtt
     7801 aatgtataac tgctgatatt ggcgcttcct gcaaggctgg tggctacgct gtaagggatc
     7861 cacacgaaat catccgtccc gccgtcttcc atgttatcct catcctgagt ctccacgata
     7921 ccaactactt taaaggcata accatttatt ttcaaggtct cattaatggc cttttctgca
     7981 cttccaaaca gttcctgggc cacataggaa ccgatcacac atactttctg ccgggaagca
     8041 atatcagaat aagcaatatt tcttccgatc tgcagatcat aatccttcat atccaggtac
     8101 tgctcgcttc tgcctgccac actggtagag tccatggagt catctccctt ttttacagta
     8161 gctgacagag atacattagg gctgatgcca tcaaaaagat ctccgtgttc atcgtaaaat
     8221 gcatacatct gatctgcatc tacagtacgg gatgtaagat tggtcatatt tacattgatc
     8281 tggtttacac ccatgctggc aaaactttcc actacgcttc ccatatagcc attgaccaga
     8341 cttaccagga taatgaccgc tgccacaccg atgatgatgc ctaacatagt caggaaagag
     8401 cgcatcttat tgccccatat gctttttata gccagtttaa aggattgcag cataatcttt
     8461 tacatctccg tcaaatattt tgtgtccatc tgcaatgcgc accacccgtt ttgcctccat
     8521 ggcaatagaa ttatcgtggg tgatcattac aatggtattt ccttcttcgt ttaactgttt
     8581 taagaaattt aaaacctcac ggctggtctt ggagtccagg gcgcctgtag gttcatctgc
     8641 caggatcagg gacggatccc cggccagagc tcttgcaata gatactcgct gctgctggcc
     8701 gccggataac tgatttggca tatttttcca tttttctgcc agacctactt tttctaaaga
     8761 agccatggcc cgctgcctgc gttcttcact tcccactcct gcatagagaa ggggaagctc
     8821 tacattttcc aggagattta actttggaag aaggttgtac tgctggaaga taaaaccgat
     8881 catcttgttg cggatccttg caagctgttt atcttccatt ttgcttacat cttcccctcc
     8941 caggtagtat tctcctttgg taggcacatc cagcgctccg ataatattca tcaatgtgga
     9001 ttttccgctt ccggattttc ccacaatcgc tacgaactca cctttgtcaa tagctacgct
     9061 tacatcatca ttggcacgga cctcttcgcc gccaagatag tagatcttga aaatgtggcg
     9121 aagatcaata agatggctgc ctggtttatc cagatctgta ccatttgtgc catcattctc
     9181 aatttgagat tttatctcat gagtattttt ctcattcaag ttgtttgttt tattctcagc
     9241 atctttccga cctttccaca aacgaaacat aaaaactcct ttcacactgc cctgctctct
     9301 tgtggataac tctatcttgg gccgccgccg tttccaccac ggtttccgcc gtcattaccg
     9361 ccaccgcttg gcgggccgcc cataccgcct ccggggccgc ccattcccgg gccacccata
     9421 aaggaattgg tagaaccggt gctggattta gccacataga ctgtatcacc ttcagaaagt
     9481 ccgctcttga tctccacata agagtcgttg gtcagacctg tttctacttc cactgcctta
     9541 aagcctgctg gaaccggtcc ctgctgctct tttacagagt catctttgat atagacctgg
     9601 ttgcctctca tcagggcatc taccgggatg gtaagaacat catttgcttc ttccaatgtg
     9661 atcacaccgt ctacattcat accaggaagc agatctccta cttcatccat ggttacagta
     9721 accggatagg tgctgacacc attaga
//
LOCUS       DBMDUA010000103         9717 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3342_length_9717_cov_3.264767, whole genome shotgun sequence.
ACCESSION   DBMDUA010000103 DBMDUA010000000
VERSION     DBMDUA010000103.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9717)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9717
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3342_length_9717_cov_3.264767"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="ACZPCL_08600"
     CDS             <1..1176
                     /locus_tag="ACZPCL_08600"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012516.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR1162561.1"
                     /translation="IVLKSHVELVVETGAVLKASEDLNDYQYISELAKRGHDHMVEVP
                     TFVNCEYDGKPRQYFIYSRDTDGVRISGGGVIDGSEEIYYGEVREDQIDGAFYPRIPL
                     ILMEHCTHLTIQNVTIRKSGFWTTHLVGCEEVEISGVRILNNRMMANCDGIDPDHCKH
                     VRISNCHIEAADDCIVLKTTEANHHYGPCEDIVISGCTLASTSAAIKIGTESVDDFKD
                     IIIDNCSIYDTNRGISFQLRDQGNIENVLINNCVIRTKNVSECWWGFAEPINIASINR
                     KDEIPSGKIKGLSITNVRCKGEGSIYIAGKDSAPIEDLTLNNIRLELEKTTKYPIKGY
                     DFRPCCGPDFQEGKINGIYVKNAKNVKAENLEVTVHDNMKEWVDKEICFENAVVKEK"
     gene            complement(1179..1382)
                     /locus_tag="ACZPCL_08605"
     CDS             complement(1179..1382)
                     /locus_tag="ACZPCL_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162562.1"
                     /translation="MEKYNFKDNSDLKNNTAENLFDDSDIQACSATDCTGLIPALPQD
                     EAEVEAYEDIYPYITKAQKTQKP"
     gene            complement(1492..3099)
                     /locus_tag="ACZPCL_08610"
     CDS             complement(1492..3099)
                     /locus_tag="ACZPCL_08610"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR1162563.1"
                     /translation="MPVKYVFVTGGVVSGLGKGITAASLGRLLKARGYKVTMQKFDPY
                     INIDPGTMNPVQHGEVFVTDDGAETDLDLGHYERFIDESLTKNSNVTTGKVYWTVLQK
                     ERRGDFGGGTVQVIPHITNEIKSRFHRNYNDNETEIAIIEVGGTVGDIESQPFLEAIR
                     QFQHDVGPGNTCLIHVTLIPYLKASEELKTKPTQASVKDLQGMGIQPDILVCRSEHPL
                     DDNIKSKIALFCNVPKTHVLQNLDVEVLYEVPLVMEEEHLAQVACECLNLPCPEPDLK
                     DWTAMIDAWKHPEKDVTVALVGKYTQLHDAYISVVEALKHGAVANRAQVHIKWVDSET
                     VTPENVDEKLGDVSGILVPGGFGDRGIDGMICAIRYAREHQVPFLGLCLGMQLSIVEF
                     ARDVIGWNDAHSVELDPATTHPVIHLMPDQDGIEDIGGTLRLGSYPCVLDKNSKAYGL
                     YGEETIHERHRHRYEVNNDYRTFLTEHGMMLSGMSPDNRIVEMIELPDHPWFVGTQAH
                     PELKSRPNRPHPLFKGFIEASLKNQDK"
     gene            3404..5422
                     /gene="ftsH"
                     /locus_tag="ACZPCL_08615"
     CDS             3404..5422
                     /gene="ftsH"
                     /locus_tag="ACZPCL_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1162564.1"
                     /translation="MDNNNKQNQNSNWQTITMLVIAALLTILIVGWMNSTVNARQRQE
                     LSYNEFVNMVENGKVESISVGSTIIEVKPKADDTNYSQQMKYYVVRLEGDYQFVDRIL
                     KNNVVTNRENNTSNALLLEILSYALPFLFLLFIMNFTMKRMGGGGIMGVGKSNAKMYV
                     QKETGITFKDVAGEDEAKESLTEIVDFLHNPGKYTKIGAKLPKGALLVGPPGTGKTLL
                     AKAVAGEAHVPFYSLSGSDFVEMFVGVGASRVRDLFKNATENAPCIIFIDEIDAIGRS
                     RDSRMGGNDEREQTLNQLLSEMDGFDSTKGLLVLGATNRPEILDPALLRPGRFDRRVV
                     VDKPDLNGRISILKVHSKDVLLDETVDFKEIALATSGAVGADLANMMNEAAINAVKNG
                     RNAVSQKDLFEAVEVVLVGKEKKDRIMSKEERRIVSYHEVGHALVSALQKHSEPVQKI
                     TIVPRTMGALGYVMNVPEEEKYLSTKKELEARLVELMGGRAAEEIVFETVTTGAANDI
                     QQATNLARAMVTQYGMSEKFGLMGLESQENQYLTGRTVLNCGDATAADIDQEVMKILK
                     NAYDEAIRLLSDDREAMDKIAAFLIEKETITGKEFMKIFREVKGLPEEKKEDGEGIPD
                     TEHLEKEDAAESPETAVAEEKENEAGAENIVADASEEKPDQSREDQQV"
     gene            5572..6213
                     /locus_tag="ACZPCL_08620"
     CDS             5572..6213
                     /locus_tag="ACZPCL_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphoribosyltransferase"
                     /protein_id="HIR1162565.1"
                     /translation="MDNRYVKIFASGSKAPLKATPGHFATNHAHINYYLDMTTLKTRL
                     SEAQEVAKHLAERFLFDKVVDTIVCLEGTQVIGAYLAEELTRAGVLSTNAHKTIYVIT
                     PEYNSNSQMVFRENILPMVHGKNVVILTAVVTTGLSLNKSIEAIQYYGGKLQSAAAVF
                     STLDELNGCPITSIYSPEDVTDYIYYDYRKCPLCEKGIKLDALVNAYGYTTLG"
     gene            complement(6228..7082)
                     /locus_tag="ACZPCL_08625"
     CDS             complement(6228..7082)
                     /locus_tag="ACZPCL_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1162566.1"
                     /translation="MTNSPSTLQGYLLEDFRLFHLKDQTSRTYSFHHHDFLKIMILLS
                     GNVTYVVEGRSYPLAPWDMVLVDRGQVHRPVVDTAQPYERILLYLSPTFLETYSTKEA
                     PLTRCFETAQYRHSQVLRLSQETLAPFKVLLQKLETNTNFRNDDFAAPLLAKALCLEF
                     LIELNRITLSPKAGFLTESTLDYRISGLLAYINSHLEEPMDVASLSALSGLSSYHMMR
                     LFKQETGYTVGNYITEKRLIKARDLMSQGTCTTQACYLCGFQNYSSFLRAYKKRYPGR
                     PKDILNSV"
     gene            7221..8078
                     /gene="dapF"
                     /locus_tag="ACZPCL_08630"
     CDS             7221..8078
                     /gene="dapF"
                     /locus_tag="ACZPCL_08630"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR1162567.1"
                     /translation="MRLKLEKYHGLGNDYLVFDPEHNGMELTPERVKLLCNRNLGVGA
                     DGILEGPVFKEGKIHMIIWNSDGSQTENSGNGIRIFAKYLKDADYVQKKDITIYTDEK
                     EIEIHYLNEEGTRLKASMGKASFWSDEVPVEGPRREIINETMVFGHIPYPVTCISVGN
                     PHLVILMDEISKDLVCRIGKYSETAKQFPEKINTQIIKVLDRTHLQTEVYERGAGYTL
                     ASGSGCCAAAAAAYRLGLTDPKMIVQMPEGTMEVEIREDGEIFLTGDVGYVGSISLGS
                     YFTEQLKLV"
     gene            8300..9637
                     /gene="glnA"
                     /locus_tag="ACZPCL_08635"
     CDS             8300..9637
                     /gene="glnA"
                     /locus_tag="ACZPCL_08635"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR1162568.1"
                     /translation="MSKYSREDIIRMVEDEDVEFIRLQFTDIFGILKNVAITASQLNK
                     ALNNDCMFDGSAIEGFVRIDESDMYLHPDLDTFEIFPWRPQQGKVARLICDVYNPDGT
                     PFIGDPRYVLKRAVKRAADMGYTFQVGPECEFFLFHTDEEGRPTTQSHERASYFDVSP
                     IDLGENVRRDIVLNLEDMGFEVEASHHEIAPAQHEIDLQYTEGLEAADHIMTFKMAAK
                     TIAKRHGLHATFMPKPKEGVNGSGMHINMSLSDKDGHNLFADDSDELGLSQLAYRFMA
                     GILGHMKEMTILTNPLVNSYKRLVPGYDAPIYIAWSPTSNRSSLIRIPSPRGEGTRIE
                     LRCPDSAMNPYLALAACLQAGLDGIEKEMELPPCIQGNLFGMEPETLKNKHVERIPET
                     LGDAIEAYRDNSFIREILGEHIYVKYLEAKEEEWRDFRAQVTDWEVNQYLYKY"
BASE COUNT         2784 a         1870 c         2470 g         2593 t
ORIGIN      
        1 atcgtattaa aaagtcatgt agaactggtg gtagaaacag gggctgtact taaggccagt
       61 gaagatctga atgattacca gtatatcagt gagctggcaa agcggggaca tgaccatatg
      121 gtagaagttc ctacttttgt aaactgcgag tatgatggaa aaccgcggca gtattttatc
      181 tattccaggg atacagacgg agtacgcatc agtggcggcg gtgtgatcga tggttcagaa
      241 gaaatttatt atggagaggt aagagaagat cagatcgatg gtgccttcta tcctcgaatt
      301 cctctgatcc tgatggaaca ctgtacccat ctgaccatcc agaatgtaac gatcagaaag
      361 agcggatttt ggaccactca cctggttgga tgtgaagaag tggagatcag cggtgtccgt
      421 attttaaata accgtatgat ggcaaactgt gatggcattg acccggacca ctgtaaacat
      481 gtacgtatca gcaactgcca tattgaggca gcagatgact gtatcgtatt aaagactaca
      541 gaggcaaatc atcactatgg tccctgtgaa gatattgtga tctccggctg tacactggca
      601 tctaccagtg ctgccattaa aatcggtaca gaaagtgtag atgattttaa ggatatcatc
      661 attgataact gcagtattta tgatacgaac agaggtattt ccttccagtt aagagaccag
      721 ggaaatattg aaaatgtttt gatcaataat tgtgtgatcc gtacaaaaaa tgtgtctgag
      781 tgctggtggg gctttgcaga gcctatcaat attgcttcca tcaacaggaa agatgagatc
      841 ccttccggaa agatcaaagg attatctatt accaatgtgc gctgcaaggg cgaaggcagt
      901 atttatattg caggaaagga cagtgcgcct atagaagatc ttacattaaa taatatccgt
      961 ctggaactgg aaaaaactac caaatatcct attaaaggat atgatttccg cccatgctgc
     1021 ggtccggatt tccaggaagg aaagatcaat ggcatctatg tgaagaatgc caagaatgtg
     1081 aaagcagaaa atctggaagt taccgttcat gataatatga aggaatgggt agacaaagag
     1141 atctgctttg aaaatgcagt tgtaaaagaa aaatgatctt atggtttctg cgttttctgt
     1201 gctttggtga tatagggata aatatcctca tatgcttcca cctctgcttc gtcctgtggc
     1261 agtgccggga tcaggccggt gcagtcggtg gctgagcagg cctggatgtc tgagtcatca
     1321 aagagatttt cagccgtatt attttttaga tcagaattgt ctttgaaatt atatttttcc
     1381 ataaaaaaac acctcctgta aacagctttt ggatcagtga tctgtcttgc tgtaaacagg
     1441 atgtgttttt ttaatagttt ttattcagcc aggtttttaa tttactgttt attatttgtc
     1501 ctgatttttt aaagatgcct caatgaatcc cttaaacagt ggatgtgggc ggtttggacg
     1561 ggacttcagt tccggatgag cctgggttcc tacgaaccat gggtgatctg gcagttcgat
     1621 catttctaca atacggttat caggagacat accggataac atcatgccat gctctgtaag
     1681 gaaagtacgg taatcgttgt ttacttcata acggtgtctg tggcgctcat ggatggtttc
     1741 ttcgccgtaa agaccgtaag ccttggaatt tttatccaga acacatggat aggaaccaag
     1801 tcttaaggta ccgccgatat cttcgatacc gtcctgatct ggcatcaggt ggatcacagg
     1861 atgagtagta gcaggatcca attctacgct gtgggcgtca ttccagccga tcacatctct
     1921 tgcaaattct acgatggaaa gctgcatgcc aaggcagagg ccgaggaatg gaacctggtg
     1981 ctctcttgca tagcggatgg cgcagatcat gccgtcaata ccacggtcgc cgaagccgcc
     2041 cggtaccagg ataccggaaa catctcctaa tttttcatct acattttcag gagttacagt
     2101 ctcagagtct acccatttaa tgtgtacctg tgccctgttg gcaacagcac cgtgcttcaa
     2161 tgcttctaca acagagatat aagcgtcatg aagctgtgta tattttccta ccagcgcaac
     2221 ggtcacatct ttttcaggat gcttccatgc atcgatcata gcggtccagt cttttaagtc
     2281 tggttccggg catggcagat ttaagcattc gcaggcaacc tgtgccagat gctcttcttc
     2341 cattacaaga ggaacctcgt aaagaacttc tacatccagg ttctgaagaa catgggtctt
     2401 tggtacatta cagaataatg cgatcttgct cttgatattg tcatctaacg gatgctcgga
     2461 acggcatacc aggatgtccg gctgaattcc cattccctgc aggtctttta cgcttgcctg
     2521 ggttggtttg gtctttaatt cttcagatgc ttttaaataa gggataaggg tgacgtggat
     2581 gagacaggtg ttgcctgggc caacatcatg ctggaactgg cggatcgcct ctaagaatgg
     2641 ctgactttcg atgtcgccaa cagtaccgcc aacttcgatg atggcgatct cggtttcgtt
     2701 atcattataa ttgcggtgga aacggctctt aatttcattg gtgatgtggg ggatgacctg
     2761 taccgtacct ccgccaaagt ctccacggcg ctctttctgc agaacggtcc agtaaacttt
     2821 accggtagtt acgttggaat ttttagtcag gctctcatcg atgaaacgct cataatgtcc
     2881 aaggtcaagg tcagtttcag cgccgtcatc ggtaacgaaa acttcgccgt gctgtaccgg
     2941 gttcatggta cctggatcga tattgatgta ggggtcaaat ttctgcatgg taactttgta
     3001 acctctggct tttaaaagac ggccaaggga agcagcggta atgccttttc caagtccgga
     3061 aacaacgccg ccggttacga atacgtattt tactggcata gtggggaacc tccttttagt
     3121 ggtaagttgg gtataggttt ttgtttacgg ttttattaaa aactgatata ttatacaact
     3181 atggaaatca gaaatctagg gacttttttc aaaaaaatgt aaaaatctta aaaaactaat
     3241 aatctaaagt ggagatgaag gggaagatcc tgcctttgtt ttcagaattt taatagttct
     3301 ttccttgatt tatggttaga ctcacattat aatagaaaag aataggctct ggcacatttt
     3361 atgcgtgcac gaaaaagcct gttagaggga gaatttatac agtatggata ataacaacaa
     3421 acagaatcag aacagtaatt ggcaaaccat taccatgctg gtgatcgctg cattgctgac
     3481 gatcctgatc gtgggctgga tgaactccac tgtcaacgcc agacagcgtc aggaactgtc
     3541 ttacaatgag tttgtaaaca tggtagaaaa tggcaaggta gagtccatca gcgttggaag
     3601 caccattatt gaggtaaagc caaaggcaga tgacaccaac tattcccagc agatgaagta
     3661 ctatgtagta cgtctggaag gagattacca gtttgtagac cgtattttaa agaacaacgt
     3721 tgttacaaac agggaaaata atacttccaa tgcattactg ttagagatat taagttatgc
     3781 acttccattc ctgttcctgc tgtttattat gaactttacc atgaagcgta tgggcggcgg
     3841 cggaattatg ggcgtaggca agagcaacgc caagatgtac gtccagaaag aaacaggaat
     3901 tacttttaaa gatgtggcag gtgaagatga agcaaaagag tctttaactg agatcgtgga
     3961 tttccttcac aatcctggaa agtacaccaa gatcggtgcg aagcttccaa agggtgccct
     4021 gttagtaggc cctccgggaa ccggtaagac ccttcttgca aaggcagtag ccggtgaagc
     4081 acatgttcca ttttattccc tgtcaggttc tgactttgtg gaaatgttcg taggtgtggg
     4141 cgcatcccgt gtccgtgacc tttttaagaa tgcaacggaa aatgctcctt gtatcatctt
     4201 tattgatgag atcgatgcca tcggacgcag ccgtgacagc cgtatgggcg gaaatgatga
     4261 gcgtgagcag actttaaatc agctgctttc tgagatggat ggtttcgact ctacaaaggg
     4321 acttttggtt cttggtgcaa ccaaccgtcc ggagatcctg gatccggctc ttcttcgtcc
     4381 aggacgtttt gaccgccgtg tagttgtaga taaaccagac ttaaatggac gtatcagcat
     4441 tttaaaagta cattccaaag atgtgctgtt agatgagact gtagacttta aggaaattgc
     4501 ccttgcaact tccggcgctg ttggtgcgga ccttgccaat atgatgaacg aggctgccat
     4561 caatgcggtt aagaacggca gaaacgctgt ttcccagaag gatctgtttg aggcagttga
     4621 ggtggttctt gttggtaagg agaagaagga ccgcattatg agcaaggaag agcgccgcat
     4681 tgtatcttac catgaagtag gtcatgcatt agtcagtgct ttgcagaaac attctgaacc
     4741 ggtccagaag atcaccattg ttccaagaac tatgggtgct ttgggatatg ttatgaatgt
     4801 gccggaagaa gagaagtatt taagtaccaa gaaggaatta gaggcaagat tggttgagtt
     4861 aatgggcggc cgtgcagctg aggagatcgt ttttgagact gttaccaccg gtgcagccaa
     4921 tgatatccag caggctacca atctggcaag agctatggtg acccagtatg gtatgtctga
     4981 gaaattcggt cttatgggac ttgagagcca ggagaatcag tatctgaccg gccgtactgt
     5041 attaaactgc ggtgatgcca cagcagcaga cattgaccag gaagttatga agatcctgaa
     5101 aaatgcttat gatgaggcaa tccgtctgtt aagcgatgac agagaagcta tggataagat
     5161 cgcagcgttc ctgatcgaga aagagaccat tacaggtaag gaatttatga agatcttccg
     5221 tgaggtgaag ggattgccgg aggagaagaa ggaagacggc gaaggtattc cggatacaga
     5281 gcatttggag aaagaggatg cagctgaaag tccggagaca gctgttgcag aagaaaaaga
     5341 aaatgaagca ggtgcagaaa atattgtagc agatgcttca gaagaaaagc cagaccagtc
     5401 tagggaagac cagcaggtat aaaactgaaa atggatctgt agatcagtga ggacagtcag
     5461 aaatggctgt cctttttgca taaaagtctt gcgaatataa tcttggtttt gtataatatt
     5521 attaaaaaat aaggggaata tcagtaactt ttctgaaaag gaggcacatt tatggataat
     5581 cgttatgtaa agatctttgc atcgggaagt aaggcacctc ttaaggcaac accgggacat
     5641 tttgctacca atcatgccca tattaattat tatctggaca tgaccaccct taagacaaga
     5701 ctcagtgaag cacaggaggt tgcaaagcac ctggcagagc ggtttttatt tgacaaggta
     5761 gtggatacga tcgtgtgtct ggaaggaacc caggtgatcg gagcttatct ggcagaagag
     5821 ctgacaaggg ccggtgttct ttctaccaac gcccacaaga ctatttatgt gatcactccg
     5881 gaatataaca gcaacagcca gatggtattt cgtgagaata ttttaccgat ggtccatggg
     5941 aaaaatgtgg tgatccttac agctgttgtg acaacgggac tgagtctaaa taagagtatt
     6001 gaggcgatcc agtattatgg cggaaaactg cagagcgcag cggctgtgtt tagtacactg
     6061 gatgagttaa acggatgccc gatcacatcc atctacagcc cggaggatgt gaccgattat
     6121 atttattatg attacagaaa atgccctctt tgcgaaaagg gaataaagct ggatgcactg
     6181 gtaaatgctt acggctatac tacacttgga taaagtgaaa caggatatta aacgctgttc
     6241 aggatgtctt tggggcgtcc tggatagcgt tttttgtatg cacgtaaaaa ggaggaatag
     6301 ttctggaaac cgcagaggta gcaggcctgt gtggtgcagg tgccctggct catcaggtct
     6361 cttgccttga tcagccgctt ttccgtaatg taatttccaa ccgtatagcc ggtttcctgt
     6421 ttaaacaggc gcatcatgtg ataggagctt aagccgctta aagcagaaag ggaagctacg
     6481 tccataggct cttccaaatg gctgttgata taggccaaca ggccggaaat gcggtaatcc
     6541 agggtgcttt ctgtgagaaa tccggctttg ggggagaggg tgatcctgtt taattcaatg
     6601 agaaattcca ggcacagggc ttttgcaagt aagggagctg caaaatcatc atttctgaaa
     6661 ttggtgtttg tttctaattt ctgaagcagg actttaaaag gagccagcgt ttcctgggaa
     6721 aggcgcagga cctgggaatg gcggtactgg gcagtttcaa agcacctggt caaaggtgct
     6781 tcttttgtgc tgtatgtttc cagaaaagtg ggagagagat agagcaggat gcgctcatat
     6841 ggttgggcgg tatctactac cggccggtgt acctgcccgc ggtctaccag gaccatgtcc
     6901 cagggagcca gtggataaga acggccttcc accacatagg tcacatttcc ggaaagaagg
     6961 atcattatct ttaaaaaatc atggtgatgg aagctgtaag tccttgaagt ctgatctttt
     7021 aaatggaaaa ggcggaaatc ttctaaaagg tagccctgga gcgtggatgg ggaatttgtc
     7081 atggaaaatc tcctgtattc attaagttcg ttattatcat ttataatagc attatttgca
     7141 atataataca gcattaaatg aatatattta tgaaataatg tggcatataa tgagtgtaga
     7201 agaacagggg aggtaaggtc atgcgattaa aattagagaa atatcacggt ttgggaaatg
     7261 attatctggt atttgatccg gaacacaatg ggatggaact gacaccggag agagtaaaat
     7321 tactttgcaa ccgcaatctt ggagtagggg cagatggtat tctggaaggt cctgttttta
     7381 aagagggaaa gatccatatg atcatctgga attccgatgg aagccagaca gaaaacagcg
     7441 gaaatgggat ccgtattttt gcaaagtact taaaagacgc agattatgtc cagaagaaag
     7501 acattaccat ttatacagat gaaaaagaga tcgagatcca ttatttaaac gaagagggga
     7561 cccgcttaaa agcctctatg ggaaaagcaa gtttctggag tgatgaggtg ccggtagaag
     7621 gtccccgcag ggagatcatc aatgagacca tggtatttgg ccacattcct tatccggtaa
     7681 cctgtatttc ggttggaaat ccccatctgg tgatcttaat ggatgagatc tcaaaggatc
     7741 tggtatgcag gatcggtaaa tattctgaga cagcaaagca gtttccggaa aagatcaata
     7801 cccagatcat caaggtgttg gaccgcactc atttacagac agaagtttat gaaagaggtg
     7861 caggttatac attggcatcc ggaagcggct gctgtgcagc agctgcggca gcttacagac
     7921 tgggacttac agatccgaaa atgatcgtgc agatgccgga aggtaccatg gaagtagaga
     7981 tccgggaaga cggggagatc tttcttaccg gcgatgtagg ttatgtggga agcatcagcc
     8041 tgggaagtta ttttacagaa cagttgaagc tggtataggc tgatatcgcc tgttgacaaa
     8101 taaaaattct actgctatat ttaaaaaaaa aggggcagtt gcaaaatgaa cggttcactt
     8161 tgcagctgcc tttttgttgt tatacatctg aaagaagctg ccggtggcag gttctgtaag
     8221 tggtatagat aaaggcacaa aagcccccag gattttgtca gatgcatggg aaaacggctg
     8281 ccagagagga agaatggata tgagtaaata cagtagggaa gacattattc gcatggtaga
     8341 agatgaagac gtagaattta tccgccttca gtttacagac attttcggta tccttaaaaa
     8401 tgtggctatt acagccagtc agcttaataa ggcattaaat aatgattgca tgtttgatgg
     8461 ttctgccatt gagggctttg tgcgcattga tgagtccgat atgtatctgc atccggacct
     8521 ggataccttt gagatctttc cgtggcgtcc tcagcagggc aaagtggcaa gactgatctg
     8581 tgatgtatac aatccggacg gaaccccttt tatcggtgat ccgagatatg tgttaaaacg
     8641 ggctgtaaag cgtgctgccg atatgggata tacgttccag gttggaccgg agtgtgaatt
     8701 tttcctgttc cacacggacg aagagggaag gcctactacc cagtctcatg agagagccag
     8761 ctattttgac gtttccccta ttgatcttgg ggaaaatgtg cgcagagata ttgtgctcaa
     8821 tctggaagat atgggctttg aggtggaagc aagccaccat gagattgctc cggcccagca
     8881 tgagatcgat ctgcagtata cagaaggtct tgaggcagca gatcacatta tgacttttaa
     8941 gatggcagcc aagaccattg ccaagcgcca tggacttcac gctactttta tgccaaagcc
     9001 aaaggaagga gtcaatggct ccggtatgca tattaacatg tccctgtccg acaaggacgg
     9061 acacaacctg tttgcagacg acagtgatga gctgggatta agccagctgg cttaccgttt
     9121 tatggcaggt attttaggac atatgaagga aatgaccatt ctgaccaacc cccttgtaaa
     9181 ctcttataag cgtctggtac cgggatatga cgcgccgatc tatattgcgt ggtcaccgac
     9241 ttccaaccgc agttccctga ttcgcatccc gtctccaaga ggagaaggaa ccaggatcga
     9301 acttcgctgc ccggactctg ccatgaaccc ttatctggca ctggcagcct gccttcaggc
     9361 cggtcttgac ggcattgaga aagagatgga gctgcctcca tgtatccagg gcaacctttt
     9421 tggcatggag ccggaaacat taaagaataa gcatgtggaa cggatcccgg aaacactggg
     9481 ggatgctatt gaggcatacc gggataattc ctttatccgt gagatattag gcgagcatat
     9541 ttatgtcaaa tatctggaag caaaagaaga agagtggaga gatttcaggg cccaggtcac
     9601 tgactgggag gtaaatcagt acctgtataa gtattaaagt tacagttcag ccatgcgctg
     9661 gtttggatga gaaagcgtgt tgcaagttta cttgcataag cactttttca tccaaat
//
LOCUS       DBMDUA010000104         9598 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3378_length_9598_cov_2.985680, whole genome shotgun sequence.
ACCESSION   DBMDUA010000104 DBMDUA010000000
VERSION     DBMDUA010000104.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9598)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3378_length_9598_cov_2.985680"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(162..1274)
                     /gene="iolN"
                     /locus_tag="ACZPCL_08640"
     CDS             complement(162..1274)
                     /gene="iolN"
                     /locus_tag="ACZPCL_08640"
                     /EC_number="3.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-scyllo-inosose hydrolase"
                     /protein_id="HIR1162569.1"
                     /translation="MGKIDWIETENPEVRFENNAIGRIKKEVWDADEAQLDKWLKEDF
                     EIPSPSELGKANCYIQTTPRHRLVERRRKNDVVFLPVGCSECHGDALPTGQDVFQVTQ
                     FLEGVRRYTAKKGYEVNLAFPITYGGHPAHHLGMPGTIVIPQDVLAEQIIAIMLGLWN
                     DGFRKIILVNNHGHQWTLATAVQEFCKRYQLPGIFQVFDWPAVCRDFFRPNNGKEFCF
                     EETFSHAGESETSLGLLMFPEMVDMDYAVDTSAKQLMKTGWYDNSISDYGRPHQWYEG
                     EGHAAIEMYGTPEGVVGTPTLSDARKAKRPVVAMCRLMVELVDNILEAFPAGTVPPTE
                     KVTLRDPESMVPYLKEPLSEGWKSVYGVPRIGAFEK"
     gene            complement(1332..2327)
                     /locus_tag="ACZPCL_08645"
     CDS             complement(1332..2327)
                     /locus_tag="ACZPCL_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1162570.1"
                     /translation="MTGTAMTMDKGLGHKIWAKIQNFQVLAGLLLLCILFSVTTPSFL
                     QMKNVMNVLNQASVNCLLSMGVLLAVLTGGNDLSTGANLALSSMIVAISIKFWGLNPF
                     VGLLLGLAVGAFIGVLNGFMLTKMHMPHPFISTLGMQNVARGLALIVTGAAPVSSFPK
                     SVQFLGNSMVGVVPMSIIFVAIVAVVFYIFLNRTAIGKHIYAIGGNAEAAKLSGINID
                     KTIIIVYTICGFMAALGGIVQMGRVDSAFPLAGTGYELNAVASVVIGGASMKGGSGTV
                     SGTIIGAMVIAVLNNGLNLLHVSTYAQQIALGIVIIGAVYVDVLRERLGSKAKKN"
     gene            complement(2299..3822)
                     /locus_tag="ACZPCL_08650"
     CDS             complement(2299..3822)
                     /locus_tag="ACZPCL_08650"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1162571.1"
                     /translation="MECPLIELRKVSKFFPGVIALNKADLKLYSGEVHGLMGENGAGK
                     STLIKLLTGAYKPDEGEMLLDGQPLKVNGPKDAMNMGLTAIYQELNVIPKLTVWENIF
                     LGRELRNKKGLLDIPQMKERAKELLQQLRQNIDVDEKVENLGMGHQQMVEICKALVLE
                     SRLIIMDEPTSSLSAGETEELLRTARELRDRGMAIVFISHHMDEIFRICDRLTVLRDG
                     CVIQSMLAKDTNTENLIKLMVGRDINQQFPKIVAAKKEELLRIEDFTSEDGSFKDISF
                     SVCGGEVLGFAGLVGAGRTEVMRAIFGADARKTGKVYVKGKEADIRSPKAAISYGIAF
                     LTEDRKGEGLVLSNTVGFNINLSTFEKSKRGLLLNNALLRNKAKNDITQLRIKTPSIN
                     TTVRGLSGGNQQKVVIAKWLETNADIFIMDEPTRGLDVGAKLEVYNIINNLVSEGKAV
                     IMVSSELPEVMGMSDRIIVMHEGYMAGEFKRDEVEREAIMMAASGGEQNDRYSNDNG"
     gene            complement(3840..4865)
                     /locus_tag="ACZPCL_08655"
     CDS             complement(3840..4865)
                     /locus_tag="ACZPCL_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR1162572.1"
                     /translation="MRRRLRELAVLVAVGGLMMAGCSSSDKKASVTDAQGKNVAESQS
                     QDEGSKKVTFVLKSLGSNYWVQLKAGAVDKAEELGIDLEVLAPSSDNATEDQIRMLED
                     QVVSGVDLVLVAPSNSGAQVATFDKVVDAGIPLICVDTNAKDYDKKTSFIGSSNEEVG
                     KKAGEYLTENVKDKSGAVILLRGQLGDETADTRSKGCTDALKAAGFSNIVEQPANNDR
                     NTAMGVMENMLQSNDNIVAVYATNDEMALGAARALQAANDKDTVVVGTNADVDALTSI
                     VEGTGVTASVRQDPYTMGAQAVETAMACLEGKEVEKMITIPVKVIDIEEAQETLDAVN
                     AVLNADK"
     gene            5106..6230
                     /locus_tag="ACZPCL_08660"
     CDS             5106..6230
                     /locus_tag="ACZPCL_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1162573.1"
                     /translation="MNTKQEAGNEKVTIYDIARQAGVSTTTVHKALHGQKGVSDAKRQ
                     EILLLAQNMNYARNTAAQNLARKELRIGVVAEVCNREFGSSIINGIKFALDQLKDSKL
                     TGYFGHLENSLSRPRVLADFRDMLNSDMDAVILFPTGPYKEYEEFNAIIEGRNIPVIT
                     INNEIPHLSCLCSIQQDGVMLGRMAGDLMNLCNPGAANAVFIGSKEVHAQNASALSFE
                     ETIAKKGGNLSSIYETQVENQIGFVLTENMLHNYPEVSGIFVGVSQCIGVIDKLKSYE
                     IVDKYKIITVDTYPEVLELLNAGIITATLDRRPYDMGQLAVHLLYQYFNSGIIPPKRI
                     LIPPSIITASAAETFNEALYPKISLLTGGIKILPPSDTEQ"
     gene            complement(6301..7650)
                     /locus_tag="ACZPCL_08665"
     CDS             complement(6301..7650)
                     /locus_tag="ACZPCL_08665"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR1162574.1"
                     /translation="MGKEVVFDYSKATGFISAEEMANFKKTVMTAKETLLSKEGAGND
                     YLGWIDLPVDYDKDEFARIKKAAAKIQSDSDVLLVVGIGGSYLGARAAIEFLSNSFYN
                     VLSKDVRKTPEIYFVGNSISSKYIADLKKVLAGKDFSINIISKSGTTTEPAIAFRVFK
                     EMLIEKYGKEEAAKRIYATTDRAKGALKNLADEEGYEEFVVPDDVGGRFSVLTAVGLL
                     PIAVCGADIDKLMEGAASGRKKALETPYEENPALLYAAVRNILLRKGKAVEIVANYEP
                     SLHYVSEWWKQLFGESEGKDQRGIFPAAVDLTTDLHSMGQFIQDGARIMFETVINVEE
                     SPEEIVLKKEDVDTDGMNYLAGKTVDFVNKSAMNGTILAHTDGNVPNLMVKVPEQNEF
                     YLGELFYFFEFACGVSGYILGINPFNQPGVESYKKNMFALLGKPGYEAQREELLKRL"
     gene            complement(7818..8594)
                     /locus_tag="ACZPCL_08670"
     CDS             complement(7818..8594)
                     /locus_tag="ACZPCL_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162575.1"
                     /translation="MMGFLQTGRALYVLAVICFGGIFVRLAAGSFYKKLIKESANMAL
                     TKNRYLRNLKQNAEDTYRINMGMNNAAVYLQRQLGNLKFLGFSLSGLENLAGQLTLLS
                     FLAGGVCAFLSYWNRSDNSYIVLYGAAGILGGMLTIVADYGVNLEGKRNQLLISLQDH
                     LENVMWPRLERESSSERASAVTEAEERRDGGTVRTIGRDRDRRLSGARRGAEQTAASR
                     ENAREASNRDKKEDRSAKDWLDDLNPEQKRMLGEMLKEFL"
     gene            complement(8828..9520)
                     /locus_tag="ACZPCL_08675"
     CDS             complement(8828..9520)
                     /locus_tag="ACZPCL_08675"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HIR1162576.1"
                     /translation="MIGIIGAMDEEVAMLKEKLTEVQVETKAAMDFYKGKLEGKDVVV
                     VRSGIGKVNAAMCAQILADVYHADHVINTGIAGSLKAEINIGDIVLSTDALQHDMDAQ
                     AFGYEPGQIPRVDTLSFKADEKMIALAKECCEKVNPEIGVFTGRVVSGDQFISDKEKK
                     KWLTDTFAGYCTEMEGAAIAQVCYFNHIPFLIVRAISDKADDSASMDYPTFEAQAIRH
                     SVNLMAEMLRCF"
BASE COUNT         2577 a         2326 c         1617 g         3078 t
ORIGIN      
        1 ccattatacc aaacggaaca ggtttatgcc ttttttattg gctgaaatat tgaattttca
       61 aggttcaaca caccacatcg gtgtgggaag ttttagttat ttattgcaaa aaatcgctta
      121 aacttatagt ctaagcgatt ttttcaaaag aaactttatg attatttttc aaatgcacca
      181 attctcggaa caccgtatac agacttccag ccttcactaa gcggttcttt taagtatggg
      241 accatagatt ctggatctcg taatgttact ttttctgtag gcggtactgt tcctgccggg
      301 aatgcttcca gaatattatc tactaattca accatcaagc gacacattgc cactaccggt
      361 ctctttgctt tacgtgcatc tgaaagtgta ggcgttccta ccacaccttc tggtgtacca
      421 tacatttcaa tagcagcatg tccttcacct tcataccact gatgcggacg tccataatca
      481 gaaattgaat tatcatacca acctgttttc ataagctgtt ttgcgcttgt atctactgca
      541 taatccatat ctaccatttc tgggaacatc agaagtccca gagaagtttc agactctcct
      601 gcatgggaga atgtctcttc aaagcagaat tcttttccat tattgggacg gaagaaatct
      661 ctgcatacag ctggccagtc aaatacctgg aaaattcccg gaagctgata acgtttgcag
      721 aattcctgta ctgcagtagc cagtgtccac tgatgaccat gattatttac aagaataatt
      781 tttcggaaac catcattcca taaccccagc ataatagcaa tgatctgctc agcaagaaca
      841 tcctgcggaa taacaattgt tccaggcatt ccaagatgat gtgccggatg tcctccatat
      901 gtaataggaa atgctaaatt aacttcataa ccttttttcg ctgtatagcg tcttactcct
      961 tcaagaaact gtgttacctg gaaaacatcc tgaccagttg gcagcgcatc tccatggcat
     1021 tctgagcatc ctaccggaag aaaaaccaca tcattttttc tacgacgctc tactaagcga
     1081 tgtctagggg tagtctggat atagcaatta gcttttccaa gttctgacgg agaagggatc
     1141 tcaaaatctt ctttcagcca tttatccagc tgtgcttcat ctgcatccca aacttctttt
     1201 ttaatgcgtc caatcgcatt attctcaaat cttacttccg gattttctgt ttcgatccaa
     1261 tcaatttttc ccattttcaa tacctcttct ttcttatcaa ttttaaatta ttttattttt
     1321 gtaattactt ctcagttctt ttttgccttt gatcccaggc gttcacgaag tacatcaaca
     1381 taaacagctc ctatgataac aatacctaaa gcaatctgct gggcatatgt agaaacatgt
     1441 aacagattaa gtccgttatt taaaactgct attaccattg ctccaataat agtccctgat
     1501 acagtgccgc ttccaccttt catggaagca ccgccaatta caacagaagc tactgcattt
     1561 aattcatatc ctgttccagc cagtggaaac gctgaatcaa cacgtcccat ctgtacaatt
     1621 cctccaagtg ccgccataaa accacagatc gtatacacaa taatgatcgt cttatcaata
     1681 ttaattcctg aaagttttgc cgcttctgcg tttccaccaa ttgcgtaaat atgttttcct
     1741 atcgctgttc tatttaagaa gatgtaaaaa acaactgcta caattgctac aaaaatgata
     1801 ctcataggga caacacctac catgctgttt cccaaaaact gaaccgactt tgggaaagaa
     1861 gagacagggg ctgctcctgt aacgatcagt gccagtcctc ttgccacatt ctgcattcca
     1921 agtgttgaaa taaatggatg cggcatatgc atttttgtaa gcataaatcc atttagcaca
     1981 cctatgaaag cgcctactgc cagtcccagt aacaatccaa caaaaggatt taatccccag
     2041 aacttaatag aaatagctac tatcatagat gaaagcgcca gatttgctcc tgttgaaaga
     2101 tcattgccgc ctgttaaaac tgctaacaaa acgcccatac tcaacaggca attaaccgaa
     2161 gcctgattta atacattcat tacattcttc atctgaagaa aacttggtgt tgttactgaa
     2221 aataagatgc ataaaagtaa caatcctgct aatacctgaa aattttggat cttagcccat
     2281 attttatgac ccagaccttt atccattgtc attgctgtac ctgtcattct gttcccctcc
     2341 tgatgcagcc atcataattg cttcacgttc cacttcatcc cgtttaaatt caccggccat
     2401 ataaccttca tgcattacaa tgatacgatc actcattccc attacttccg gcaattccga
     2461 agacaccata atgactgcct ttccctcaga aaccagatta tttataatat tgtacacttc
     2521 aagtttcgct ccaacatcca ggccacgcgt aggctcatcc atgataaata tatctgcatt
     2581 tgtttccagc cattttgcga tcactacttt ctgttgattt cctccagata atcctcttac
     2641 agtagtatta atagacggcg ttttaatccg taattgggta atatcatttt ttgctttgtt
     2701 tcttaataat gcattattta gcaacagtcc ccgcttactt ttttcaaagg tactgagatt
     2761 tatattaaat cccactgtat tggatagcac cagcccttcg ccttttctat cttctgtaag
     2821 aaacgcaatt ccatagctga tcgcagcttt cggagatcga atatccgctt cttttccctt
     2881 aacatatact tttccggttt ttctggcatc tgctccgaaa atagctcgca ttacttctgt
     2941 tctgcctgct cctaccaacc cagcgaatcc tagaacctca ccgccacata ctgaaaagga
     3001 aatatcttta aaggatccat cctctgaagt aaaatcttca attctgagaa gttcttcttt
     3061 tttagcagca actatcttag gaaactgttg attaatatca cggcccacca taagttttat
     3121 aaggttttct gtatttgtat cttttgccag catcgactgg atcacgcatc catctcgaag
     3181 cactgtcagg cgatcacata ttctgaaaat ctcatccata tggtgagata taaaaacaat
     3241 agccattccc cgatcacgta attcccgtgc tgtacgaagc agttcttctg tttcacctgc
     3301 actcaacgaa gaagttggtt catccataat gatcaaacgt gattccaaaa ctaatgcttt
     3361 acagatctcg accatttgct ggtgtcccat tcccagattt tctacttttt catctacatc
     3421 aatgttttgc cgcaactgct gtaacagttc tttcgcacgt tctttcattt gaggaatatc
     3481 taatagtcct tttttattcc tcagttctct tcccaaaaat atattttccc atacagttag
     3541 cttagggatt acattaagct cctggtaaat tgccgttaag cccatattca tcgcatcttt
     3601 gggaccattt accttaagag gctgtccgtc taaaagcatt tcgccctcat ctggtttata
     3661 tgctcctgtt aagagtttga tcaacgttga cttgccagct ccattttctc ccatcaagcc
     3721 atgcacttca ccggaatata atttcagatc cgctttgttc aaagctataa cacctgggaa
     3781 aaattttgaa acttttctta attcaatcag cggacattcc atgctcttcc tctttcctgt
     3841 tatttgtctg cattaagaac tgcatttact gcatccaaag tttcctgcgc ttcctcaata
     3901 tcaattacct ttaccggtat tgtgatcatt ttttcaactt cttttccttc taaacaagcc
     3961 attgctgttt caactgcctg agctcccatt gtatatggat cctgtctaac agacgctgtt
     4021 acgcctgtac cttctacaat tgatgtaaga gcatccacat ctgcgttagt tcccactaca
     4081 actgtatcct tgtcgtttgc tgcctgcagc gctcttgctg ctcccaatgc catctcatca
     4141 tttgtagcgt atacagcaac aatattgtca tttgactgaa gcatattttc cataacaccc
     4201 attgccgtat tccggtcatt attcgcaggc tgttctacaa tattggaaaa accagctgcc
     4261 tttaatgcgt ctgtacatcc tttacttctg gtgtcagcag tttcatctcc caactgtcct
     4321 ctcagtaata taactgctcc ggatttatct tttacattct ctgtcagata ttcccctgct
     4381 ttctttccaa cttcttcatt agatgatccg ataaatgatg ttttcttatc gtaatccttt
     4441 gcatttgtat ctacacagat caaaggaata cctgcatcaa ccaccttatc aaatgtcgct
     4501 acctgtgctc ctgaattcga aggagctacc aataccagat caaccccact tacaacctga
     4561 tcctcaagca tacggatctg atcttctgtt gcattatctg agctaggtgc cagtacttcc
     4621 aaatctatac ccagttcttc tgctttgtct acagccccag cttttaactg tacccagtaa
     4681 ttgctaccta gtgactttaa aacaaatgta actttcttgc taccttcatc ctgactctgg
     4741 ctttcagcaa catttttccc ctgagcgtca gtcactgatg cttttttatc agatgaactg
     4801 catcctgcca tcataagccc accaactgca accaataccg ctaattctct taaccttctc
     4861 ctcataatgg aacctccttt atttaggtaa gcgcttaact ttaattagag attatcataa
     4921 tttatttttc gtttctattg acattattga taatattatc tcatttatta tgtgcatttt
     4981 tcacaatatt gttttactct tttactcttt tttaaaatat catttctttg ttgggtaatt
     5041 tttacttgct tttcttttat ttcaatggta taatttactc atctcatata ggaggatctt
     5101 attatatgaa tactaagcag gaggctggaa atgaaaaggt aactatctac gatatagccc
     5161 gtcaggcagg tgtctctaca accactgtcc acaaagctct tcatggtcaa aaaggcgtca
     5221 gtgatgcaaa acgtcaagaa attttacttc ttgcgcaaaa tatgaattac gccaggaata
     5281 ccgccgcaca aaaccttgcc cgaaaagaat tgcgcattgg tgttgtagca gaagtttgta
     5341 accgtgaatt cggaagtagt atcattaatg gaattaaatt tgcattagat caattaaaag
     5401 attcaaagtt aactggatat tttgggcact tagaaaattc tcttagtcgg ccacgtgtcc
     5461 tggctgactt tcgcgatatg ctcaattccg atatggatgc tgtcattctt tttcctactg
     5521 gtccatataa ggaatacgaa gagtttaatg ccataataga agggcgaaat attcctgtta
     5581 ttacaatcaa caatgaaatt cctcaccttt cttgtttatg ctcaatccag caggatggtg
     5641 ttatgctcgg ccgcatggcg ggtgacctta tgaatttatg caatccagga gccgctaatg
     5701 ctgttttcat tggaagcaag gaagttcatg cccaaaacgc ctctgccctc agttttgaag
     5761 aaactattgc gaaaaaggga ggaaatctaa gttccatata tgaaactcaa gtagaaaatc
     5821 agattggctt cgtattaacg gaaaatatgc ttcacaatta cccggaagta agtggcattt
     5881 tcgttggcgt ttctcaatgt attggggtaa ttgataaact taagtcttac gaaatcgtag
     5941 ataaatataa gataattact gtagacactt atcctgaagt tcttgagctt ttaaacgcag
     6001 ggattattac tgccacttta gatcggcgtc cctatgatat ggggcagctg gcagttcatc
     6061 tgctttatca atactttaat tccgggatca ttccacctaa gcggatttta atcccacctt
     6121 ctatcataac cgcaagcgca gcagaaacat ttaatgaagc tttataccct aaaatttctc
     6181 tgttaacagg tggaatcaaa attcttcctc ccagtgatac tgagcagtaa atacgcttaa
     6241 gcaaaaaagt tccagtcatt taaacatggc tggaactttt ttcttgcttt ttattatatt
     6301 ttacagtctc tttaacagct cttctctctg agcttcatag cctggctttc caaggagagc
     6361 aaacatgttc ttcttgtagc tttctacgcc tggctggtta aatggattaa ttcctaaaat
     6421 gtagccgctt acgccgcagg caaactcaaa gaagtagaat aattcaccta aatagaattc
     6481 attctgctct ggtaccttaa ccatcaggtt tggtacattt ccgtctgtgt gagccagaat
     6541 ggttccgttc atagcgctct tatttacgaa atctacagtc tttccagcaa gatagttcat
     6601 gccgtcggtg tctacatcct ctttctttaa aacgatctct tccggagatt cttctacgtt
     6661 gataacggtc tcaaacatga tgcgtgcgcc gtcctggata aactgtccca tggagtgcag
     6721 gtctgtggtc agatctactg ctgcagggaa gatacctctc tggtcttttc cttcgctttc
     6781 gccaaacagc tgcttccacc actcagatac atagtgaagg cttggctcat agtttgctac
     6841 gatctctact gctttgccct tgcgaagcag gatgttgcgg actgctgcat acagaagagc
     6901 cgggttttct tcgtaaggag tttccagtgc tttcttacgt ccggaagcag caccttccat
     6961 cagcttgtca atatcggcac cgcatacagc gatcggaagc agacctactg ctgtaagaac
     7021 ggagaaacgt ccgcctacat catccggaac tacgaattct tcatagcctt cttcgtctgc
     7081 caggttcttt aaagcaccct ttgcacggtc tgtggttgcg tagatacgct ttgcagcctc
     7141 ttctttgcca tatttttcga tcagcatttc cttaaataca cggaatgcga tagccggctc
     7201 tgtggttgta ccggacttag aaatgatatt gatggagaaa tcctttccag ccaaaacttt
     7261 cttaaggtct gcaatatatt tgctgctgat gctgtttcct acaaaataga tctcaggagt
     7321 cttgcgcaca tccttagaca gcacgttata aaagctgttg cttaaaaatt cgatggctgc
     7381 tcttgctcct aaataggaac cgccaattcc tactaccagc agaacatctg aatcgctctg
     7441 gatctttgct gctgcctttt taattcttgc aaactcatcc ttatcataat caactggaag
     7501 atcgatccaa ccaagatagt cgtttccagc accttctttg cttaacaaag tctcctttgc
     7561 tgtcataact gtttttttga agttagccat ctcttcagct gagataaaac cggtggcttt
     7621 tgaataatcg aatacaactt ccttacccat gttcccgctc tccttttctg attgcatact
     7681 tgtgagattt gtgaaaattt tcccaactaa tttttagtat agcatatttg tgttgtcaat
     7741 tccataacct gcgctgattt tctacgactg ttttcaaacg ctgtttttaa caccgccttc
     7801 aaaccacagg cttttattta cagaaactcc ttcagcatct ccccaagcat ccgtttctgc
     7861 tccggattta agtcatccag ccagtctttg gccgaccggt cctctttttt atcccggttt
     7921 gatgcctcac gggcattttc cctgctggct gccgtctgct ctgcgcccct tcttgcacca
     7981 gacaaccgac gatcccggtc ccttccaatg gtccgcacag ttccaccatc acgccgttct
     8041 tcggcctctg taactgcact tgcacgctcg ctgctgcttt ccctctccag tcttggccac
     8101 attacatttt ccagatgatc ctgaaggctg atgagaagct ggtttctctt tccttccaga
     8161 tttacaccat aatcagccac gatcgtaagc atcccgccaa ggattcccgc tgctccgtaa
     8221 aggacaatgt aagaattgtc actgcggttc caataggaaa gaaaagcgca tacaccgcct
     8281 gccagaaaac tgagcagagt cagctggcct gccagatttt caagtcccga taaagaaaaa
     8341 cctaaaaatt taagatttcc aagctgcctc tgtaaataaa cagcagcatt gttcatgccc
     8401 atgttaattc tatatgtatc ttctgcattc tgttttaagt tccgcagata ccggttcttg
     8461 gtcagcgcca tatttgcgct ttcttttatc agttttttgt agaagcttcc ggctgccagc
     8521 cgtacaaaaa tacctccaaa gcagataact gccagcacat acagtgctct tcccgtctgt
     8581 aaaaagccca tcataatata agctcccctt tctgtttcca ctgtctgcaa atgttattat
     8641 ttttattgca gaatgattat tccggccgac acacctgcag gcctgtgtca ttcggtaagc
     8701 ttaattataa cgacggcttt ttatttggga agttgagaac ccttaaaatc gttcgtcaaa
     8761 ttctcccacg aaacgattct gaacagggtt taaaagacat tgataactgg ctgtggatca
     8821 tcttgtatta aaaacagcgg agcatctctg ccattaagtt cacactgtgg cggatggcct
     8881 gtgcctcaaa agtcgggtaa tccatagaag cactgtcatc tgccttatca gaaatcgctc
     8941 ttacaataag gaatggaata tggttaaaat agcatacctg ggcaatagct gcaccttcca
     9001 tttctgtgca gtagcctgca aaagtatcag taagccattt cttcttctct ttatcagaaa
     9061 tgaactggtc tccggaaaca actcttcctg taaatacgcc gatctccggg tttacttttt
     9121 cgcagcactc ttttgcaaga gcgatcattt tctcatctgc cttaaaagaa agtgtgtcta
     9181 ctcttgggat ctgtcctggc tcatagccaa atgcctgggc atccatatca tgctgtaatg
     9241 catctgtaga cagcacgata tccccaatgt tgatctctgc ctttaaggaa cctgcaatac
     9301 cagtgttgat cacatggtct gcatggtata catctgccag gatctgtgca cacatagctg
     9361 catttacctt accgataccg gaacggacaa ctactacgtc ctttccctct aattttcctt
     9421 tataaaagtc catagcagcc tttgtttcta cctgaacctc tgtgagtttt tcctttaaca
     9481 ttgccacttc ttcatccatg gccccgataa ttccgatcat tcttttcctc caacttgaca
     9541 atcttattta catgaagcat aatgaaaggt acactgttgc tggtcatgca acaacatg
//
LOCUS       DBMDUA010000105         9595 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3380_length_9595_cov_3.205709, whole genome shotgun sequence.
ACCESSION   DBMDUA010000105 DBMDUA010000000
VERSION     DBMDUA010000105.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9595)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3380_length_9595_cov_3.205709"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            254..1564
                     /locus_tag="ACZPCL_08680"
     CDS             254..1564
                     /locus_tag="ACZPCL_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1162577.1"
                     /translation="MKDLTKGSPSKVMISFAIPVALGNIFQLCYSLADTRVVGSTLGE
                     AALAAVGATTSISTLFIGFLSGLTNGFALLIARQFGAGDKKGVTRYGAGCLLLGCITS
                     VLLTFICVSSLPFILRLLNVPEALMGQSTAYIRAVLLGLLATMLYNGCASILRAVGDT
                     TAPLLFLLFAAALNVALDLLFILKFGFGVAGAAWATVLSQSLSAIISLVYMFKRYPVF
                     RFKPDDFKLKLREIKALYTSGLSMAAMMSLVFFGTLSLQCAINTFGQDIIVAHTAARK
                     ITEFFMLPFSVMGVTMATYCGQNMGAGRKDRIRTGICQALILTWIWSLGMILLSYTVA
                     PWLIWLVTGSKNPAVLSNATWYLKTDTLFYFAPAAFSILRNGLQGMGDHITPVFSSMI
                     ELIGKVICAFLLARIFQYWGIIMAEPIVWILMVIPLIIKARKLL"
     gene            complement(1628..3271)
                     /locus_tag="ACZPCL_08685"
     CDS             complement(1628..3271)
                     /locus_tag="ACZPCL_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR1162578.1"
                     /translation="MSNLKPMLLTALKSYDKSQFVKDVTAGIIVAIIALPLSIALALA
                     SGVGPEAGIFTAIVAGFVISALGGSSVQIAGPTAAFATIVAGIVARDGMDGLVIATVL
                     AGVFLILMGICRFGSLIKFIPYTITTGFTSGIAVTIVIGQLKDFFGVTYPEGVRPIET
                     MEKLAAFLENIGTVNPAALIVGAVSLAILIISPYIFKKIPGSLIAVIVGILMVKFLPL
                     KVSTIGNLYTISNALPSFHLPTVNFEIVQAAIPNAFTIAILAAIESLLSCVVADGMIN
                     GKHRSDMELVAQGAGNIASALFGGIPATGAIARTAANIKNGGRTPVAGMVHSITLVIV
                     LVVLMPYAGMIPMPTIAAILFIVAYNMCQWRTFLQLVKTAPKSDILVLVVTFVLTVLF
                     DLVVAIEIGMILACLLFIKRMSEETKAESWVYTDDDTVAVNENLRKLPAEIRVYEVSG
                     PLFFGASDAIEHIVVEESAKCLVLRMRAVPALDSTAMNALTALTKTCESKGVTIVFSH
                     VNEQPMKVMKKAGFVALAGEENFCPNITAALERAEEIIK"
     gene            3769..4269
                     /locus_tag="ACZPCL_08690"
     CDS             3769..4269
                     /locus_tag="ACZPCL_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIR1162579.1"
                     /translation="MENQTFTNVRKLTTSGIVIALYVVILFMTQSFSFGAYQIRIVTS
                     LYALSYLFPFLVIPLGLANFIANMLGGFGIIDMVGGFIVGIVTSGIVMLIKKFHLPRF
                     MIFFPITLVPGLGVALWLSPMVHMPYGALALSLCIGQTVPAVCGVALSKALEQVFYPK
                     HSISNA"
     gene            4282..4779
                     /gene="queF"
                     /locus_tag="ACZPCL_08695"
     CDS             4282..4779
                     /gene="queF"
                     /locus_tag="ACZPCL_08695"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HIR1162580.1"
                     /translation="MTGRNEKELEGVSLLGNQTNVYRADYAPEVLETFPNKHPENDYF
                     VKFNCPEFTSLCPKTGQPDFATIYISYVPGERMVESKSLKLYLFSFRNHGDFHEDCVN
                     IIMKDLIRLMDPKYIEVWGKFTPRGGISIDPYCNYGKKDTLWEKVAFDRLTHHDMYPE
                     KIDNR"
     gene            complement(4776..6629)
                     /locus_tag="ACZPCL_08700"
     CDS             complement(4776..6629)
                     /locus_tag="ACZPCL_08700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR1162581.1"
                     /translation="MAIAANTDQKRENLLNLSLSVSEEERMRSESLETGFDREERTWE
                     LIVKYFGDLSGLRERGIQVRELLSGYGILKVPEYLVDFVTNLPEIEYIEKPKRLFFAV
                     NQARAASCLLDVQQGIGGENANSDPLENIFGMDGGTVTERVSAETKLPTDLTGKGVLI
                     AIIDSGIDYLHPDFQNPDGTSRILYLWDQGRDIVYTKEEINEALSAYREGSGGRNRAA
                     ALQIVPSADTSGHGTAVAAIAAGNGRGSSGLYRGVACESELMVVKLGIPLADNFPRTT
                     QLMEAVDFVLRTARQLGRPVAVNLSFGNTYGSHDGTSLLETFLDDMTGYGRNVIVAGT
                     GNEGAGAGHTGGLLEMGREQVIELSVSAFENSFGVQLWKSYADIFSISLRSPSGAIMG
                     TLQENLEKQRFRSENTEILSYYGKPSPYSMAQEVYFDFIPAGDYIDSGIWQFILTPER
                     IITGQFDLWLPSKAALNNDTRFLKSTPETTLTIPSTARKIISAGAYDFRTRAYADFSG
                     RGFTRQTNQVKPDLVAPGVNIITAGNRDGYEAVTGTSFAAPFVTGAAALLMQWGIING
                     NDPFLYGEKVKACLIRGARHLPGYDIWPNARVGYGSLCVSESLPLRSGGEG"
     gene            complement(6716..7636)
                     /locus_tag="ACZPCL_08705"
     CDS             complement(6716..7636)
                     /locus_tag="ACZPCL_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufB/SufD family protein"
                     /protein_id="HIR1162582.1"
                     /translation="MMDQIQERLLAEVADLHGVPEGAYNIRANGKMAGRSTTANIDIT
                     TKTDKPGIDIKIKPGTKHESVHIPVVVSASGLKEMVYNDFYIGEDSDVVIVAGCGIHN
                     CGDQDSEHDGIHRFFIGKNAKVKYVEKHYGEGDGTGKRVLNPGTEVYMEENSQMEMEM
                     VQIKGVDSTIRTNTAKLAAGARLVVRERLLTHGAQVAESIYEVELNGEGSSADVVSRS
                     VAKGTSKQTFRASIAGNAKCSGHTECDAIIMDDAKILAVPGLEANDVDAALIHEAAIG
                     KIAGEQLIKLMTLGLTEEEAENQIINGFLK"
     gene            complement(7633..8385)
                     /locus_tag="ACZPCL_08710"
     CDS             complement(7633..8385)
                     /locus_tag="ACZPCL_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1162583.1"
                     /translation="MLELKHLSFEVNDDKGEKGIIKDISLKVNDGKFVVITGPNGGGK
                     STTAKLIMGIERPTGGQIFFDGEDITEKNITERARMGISFAFQQPVRFKGVQVLDLLR
                     LASGKKLSAAQACQYLSEVGLCAKDYINREINASLSGGELKRIEIATVIARNAKLSVF
                     DEPEAGIDLWSFQNLIQVFERMRKNNNGSILIISHQERILNIADEIVVIADGKIVKQG
                     TREEIMPEIMGTAAAVGVCSGFTGNDGKEDQE"
     gene            complement(8501..8584)
                     /locus_tag="ACZPCL_08715"
     tRNA            complement(8501..8584)
                     /locus_tag="ACZPCL_08715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8548..8550),aa:Leu,seq:cag)
     gene            complement(8739..>9595)
                     /locus_tag="ACZPCL_08720"
     CDS             complement(8739..>9595)
                     /locus_tag="ACZPCL_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009662211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIR1162584.1"
                     /translation="PYVYYNEHCIIFNEAHRPMKIDQAVFRKLLEFVKLFPHYTAGSN
                     ADLPIVGGSILSHDHFQGGGYVFAMAKAPYESEFVIPGYEDLTAGIVRWPMSVIRLRG
                     TDTERITLAADHILTAWRRYTDKEAFIFSETDGTPHNTITPIARMHGNQYELDLVLRN
                     NVTTDECPWGVYHPSADLHHIKKENIGLIEVMGLAVLPARLKKEMALLEQYILEKQDV
                     RSNPQIEKHADWVDSWLGSYEITEDNIHAILQKEIGKVFVQVLECAGVYKNTKKGRID
                     FGRFIETL"
BASE COUNT         2461 a         2407 c         1878 g         2849 t
ORIGIN      
        1 gactatatag taaaacgaaa aggcaccgct gaaaaaccca ggtagaataa ggctttcagc
       61 ggtgttttga taaatttgac tgtcaaagat gggattatac actatttttg ttaccactac
      121 atcatcttat aacgatttta atccaaaaag taacctctcc atgtacagta atattttctt
      181 ccattgatta ccctgcattt ttatggtacc atcagaactg ttaaaattaa aaaaatgaag
      241 aaagcgtatt tttatgaaag acttaacaaa gggttctccc tcaaaagtaa tgatctcctt
      301 tgccattcct gtggctcttg gaaatatttt ccagttgtgt tacagtctgg ctgacacccg
      361 cgtggtaggc agtaccttag gcgaagctgc tctggcggct gtaggagcta ctacttctat
      421 cagcacttta tttatcggat ttctgtccgg acttaccaat ggttttgccc ttctcattgc
      481 ccgccagttt ggtgccgggg ataaaaaagg cgtaacccga tatggcgccg gatgtctgct
      541 gttaggatgc atcacatctg tgcttcttac cttcatctgc gtaagcagtc ttccctttat
      601 tttacgactg cttaatgtac ctgaagcatt aatgggccag tccaccgcct acatacgtgc
      661 tgttctttta ggacttttag ccactatgct ttataatggc tgcgcatcta tcctgcgggc
      721 tgtgggcgat accactgcgc cccttttatt ccttctgttt gcagcagcac taaatgtagc
      781 cctggacctg cttttcattt taaagtttgg ttttggtgtt gcaggcgctg cctgggccac
      841 cgtattgtcc cagtcacttt ccgccatcat aagtctggtc tacatgttta agagatatcc
      901 tgttttccga tttaaaccag atgattttaa acttaaactg cgggaaataa aagctttata
      961 tacctccggc ctttccatgg cagccatgat gtctctggtg tttttcggaa ccttatccct
     1021 tcaatgtgcc atcaatacct ttggtcagga tattatcgtt gcacacacag ccgcccgcaa
     1081 gatcacggaa tttttcatgc ttccattctc tgttatgggc gttaccatgg caacctactg
     1141 cgggcagaat atgggagcag gacgtaaaga ccggatccgc acaggtatct gccaggctct
     1201 gatccttacc tggatctggt cccttggaat gatcctttta agctacacag ttgccccatg
     1261 gcttatatgg ctggtaacag gatctaaaaa tccggctgtc ctgtctaatg caacctggta
     1321 tttaaaaacg gacactctct tttattttgc tcctgctgcc ttttccattt taagaaacgg
     1381 gcttcaggga atgggcgacc acattactcc tgtattttcc agtatgatcg aactcatagg
     1441 aaaagtgatc tgtgctttcc tccttgcccg catcttccag tactggggca ttattatggc
     1501 cgaacctatc gtatggatct taatggtcat tccacttatt ataaaagcca gaaagctgct
     1561 gtgacatctg atcacaacag ccttccggct ttttcttttt ttatgtattt ttctgttatg
     1621 tactgcttta tttaatgatc tcttctgctc tttccagtgc agcagtgata ttcgggcaga
     1681 aattctcttc acctgcaaga gcaacaaatc cagccttttt cattaccttc ataggctgct
     1741 catttacatg ggagaataca atggtcacgc ccttgctttc gcaggtcttt gtaagtgcag
     1801 tcagggcatt cattgctgtg ctgtccagtg caggaacggc tctcatacgc agtaccaggc
     1861 actttgcgct ctcttccact acaatgtgct caatggcatc agaagcgcca aagaacagag
     1921 ggccgcttac ttcgtaaaca cggatctcag ccggaagctt gcgcaggttt tcattaacag
     1981 ctacagtatc atcatctgta tatacccagc tttctgcctt tgtctcttcg ctcattcttt
     2041 tgatgaacag cagacatgca aggatcatac ctatttcaat agccacgacc aggtcaaata
     2101 atacagtcag tacaaaagtt actaccagaa ccaggatatc gctctttgga gctgttttta
     2161 ctaactgtaa gaaggtacgc cactggcaca tattataagc tacgataaac aggatcgctg
     2221 caatggtagg cattgggatc atacctgcat atggcatcag cacaaccagt acgatcacca
     2281 gggtaataga gtggaccata ccagcaactg gtgtacggcc gccgttttta atgtttgctg
     2341 ctgtacgtgc aatggcgcct gtagccggaa taccgccaaa taatgcagaa gcaatgtttc
     2401 ctgcgccctg cgctactaat tccatatcag aacggtgttt tccattgatc atgccgtctg
     2461 caaccacaca ggaaagcagg gactcaatag ctgccaggat agcaatggta aacgcatttg
     2521 gaatagccgc ctgcacgatc tcaaagttca ctgtcggcag gtggaaagat ggcagtgcat
     2581 tgctgatagt gtacagattt ccaatggtag aaacctttaa aggcaggaat tttaccataa
     2641 gaataccaac gataaccgcg atcagggaac ctgggatctt cttaaagata taaggagaaa
     2701 taatcaggat cgccagactt acagctccta cgatcagtgc cgcaggatta acagttccaa
     2761 tattttccag aaatgctgcc agcttttcca tggtctcgat cggacggaca ccttccggat
     2821 aggtcacacc aaaaaagtcc tttaactgtc cgatgacaat ggtaacagcg ataccggatg
     2881 taaaaccggt tgtaatggtg tacggaataa atttgatcag gcttccgaaa cggcagatgc
     2941 ccatcaagat caggaataca cctgcaagaa ccgttgcgat cacaagaccg tccattccgt
     3001 ctcttgctac gatacctgct acaatggtag caaatgctgc tgtcggtcca gcgatctgta
     3061 cactgcttcc gcccaatgcg gagataacga atccagctac gatagccgta aaaatacctg
     3121 cctcaggacc tacgccagat gccagtgcca gagcgataga cagaggaagc gcgatgatcg
     3181 ctacgataat acctgctgtc acgtctttta caaactggga tttgtcatag gactttaagg
     3241 ctgttaataa catgggtttt aagttgctca ttttaccttc gtcctttcat tttcctttat
     3301 ttctgctttt ttattatttc atactcacga tccccgcgct ttcgcgctcc actgcctgct
     3361 gacgcaggcg gctcgttcgt taatgaccca agaaaaacaa atgtctgctg cgcagccgct
     3421 tgtttttctt ccccggccat tatatcataa agaaaatgtt aagaaaccat tattttccat
     3481 gtttttatta aaatacattt cccctgcttc acgcttcatc tgcaaaagcc agctcttcca
     3541 cagtcatcaa atatggcaca tgcttatcac atactcatca catactcatc cggaagataa
     3601 ttaaaaaact ccttgctttc atctccattt tgttctatac tactcaggtc tgtaacttcc
     3661 agatacgatt gacagttcga gaccatcctg tcacaaaata aaactaggga caacaaatat
     3721 taactatgat caattgttgt tgtgattata ttttggaggt gaacaaacat ggaaaatcaa
     3781 acttttacta acgtccggaa acttacaact tccggcattg tgatcgcgct ttatgttgtt
     3841 attttattta tgacacaaag tttttctttc ggggcttatc agatccgtat tgtaacttct
     3901 ttatatgccc tttcttatct ttttccgttc ctggttatcc cgcttggtct cgctaatttc
     3961 atcgcaaata tgcttggagg tttcggcatc attgacatgg tcggaggttt tatcgtcggc
     4021 attgtaacat ctggtattgt catgctgatc aaaaaattcc acctgccgcg ttttatgatc
     4081 tttttcccga tcacgcttgt accgggcctt ggtgtcgcac tatggctgtc acctatggtc
     4141 cacatgcctt atggggcact ggcacttagc ctgtgtatcg ggcaaacagt acctgctgta
     4201 tgcggcgtag cactgtccaa ggcacttgaa caggtatttt acccaaaaca ttctataagc
     4261 aacgcatagg gggatatgaa aatgactgga agaaacgaaa aagaactgga aggcgtcagc
     4321 cttttgggaa accaaaccaa tgtatacagg gcagactatg ctccggaagt cctggaaact
     4381 tttcccaata aacacccaga aaatgattat tttgtaaagt ttaactgtcc tgaatttacc
     4441 agcctttgtc caaaaaccgg acagcctgat tttgccacga tctatatttc atacgttccc
     4501 ggtgaacgca tggtggagag taaatccttg aaattgtatc tgttcagttt ccgcaatcat
     4561 ggtgactttc atgaagattg tgtcaatatt attatgaaag acctgatccg cctgatggac
     4621 ccaaaatata ttgaagtctg gggaaaattc acccctcgcg gcggtatatc cattgatccc
     4681 tactgtaact atggaaaaaa agatacatta tgggaaaaag tagcttttga ccggctgaca
     4741 caccacgata tgtatccgga aaagatcgat aatcgttagc cctctccccc actccgcaaa
     4801 ggaaggcttt ccgatacaca taaactgcca taccccaccc gcgcatttgg ccagatatca
     4861 tatcccggta aatgtctggc accgcggatg aggcaggctt ttactttttc accgtataga
     4921 aacggatcgt ttccgttgat gatcccccat tgcatcaaaa gggctgccgc tccggtaaca
     4981 aagggcgctg cgaaagatgt tcctgtaaca gcttcgtagc cgtccctgtt tccggctgtg
     5041 ataatattca cccccggagc caccagatct ggttttacct gattggtctg ccgggtaaaa
     5101 ccacggcctg agaagtcagc ataagctctt gtgcgaaagt cataggcgcc tgcagaaata
     5161 atttttctgg ctgtagaggg aatagtcagt gtagtttccg gggtactttt taaaaagcgg
     5221 gtatcattat ttaatgcagc tttggaagga agccataaat caaactgacc ggtaatgatc
     5281 ctttccggtg tcaggataaa ctgccagatg ccgctgtcta tataatctcc tgctggtata
     5341 aaatcaaaat atacctcctg cgccatgctg taaggagaag gttttccata ataactcagg
     5401 atctctgtat tttcacttct aaaacgctgt ttctccagat tttcctgtaa agttcccata
     5461 atagcacctg aaggcgatct tagggaaata gaaaatatat ctgcatagga tttccacaac
     5521 tgcaccccaa aactattttc aaaggctgat acagacagct ctatgacctg ctctcttccc
     5581 atttccagca gaccgcctgt atgacctgct ccggctccct catttccggt ccccgctacg
     5641 atcacatttc tgccatatcc tgtcatatca tctaaaaagg tttctaaaag actggttccg
     5701 tcgtgagaac cgtatgtatt tccaaagctt aaatttactg ccaccggccg tcccagctgt
     5761 ctggctgtgc gaagaacgaa atctactgct tccattaact gggtagttct gggaaaatta
     5821 tctgcaagag ggatacccag ttttaccacc atcagctcac tttcacaggc tacaccacgg
     5881 tatagcccgc tgcttcctct gccatttcct gctgcaatag cagctactgc cgttccatgg
     5941 ccacttgtat ccgcagaagg aacaatctgc agtgccgcag ccctgtttcg ccccccagaa
     6001 ccttcccggt atgctgacag agcctcattt atctcctctt tcgtgtatac aatatcccgg
     6061 ccctgatccc atagatacag gatgcggctg gtgccgtctg gattttgaaa atccggatga
     6121 aggtaatcaa taccggaatc gataatagca atgagaacgc cttttccagt cagatccgtt
     6181 ggcagcttag tctctgctga tactctttcc gtcacagtgc ccccgtccat accaaatata
     6241 ttttctaaag ggtctgaatt tgcattttct ccaccaatcc cttgctgcac gtccaataaa
     6301 caagaggccg ctcttgcctg atttacagca aagaacagcc tcttaggttt ttctatatat
     6361 tcaatttccg gtaagtttgt aacaaagtcc accagatatt ccggcacctt cagtatacca
     6421 taaccactta acaattcccg tacctggatg ccccgttccc gcaatccact aagatcacca
     6481 aaatacttaa caatcagctc ccaggtccgt tcttcacggt caaagccggt ttccagactt
     6541 tcagaacgca tcctctcttc ttctgataca cttaaagaca gatttaaaag gttttccctc
     6601 ttctggtctg tattggctgc aattgccata tctgttatat tttcctgctt tcttacgccc
     6661 tttccctaat acttatgcat caaaacatag ttaatatgac attttcagat ctgtcttact
     6721 tcaaaaatcc attaatgatc tgattctctg cctcttcctc agtcagtccc aatgtcatca
     6781 gtttgatcag ctgctcaccg gcaatctttc cgatagctgc ctcatggatc aaagctgcat
     6841 ctacatcgtt tgcttccagt cccggaactg ccaggatctt tgcatcgtcc atgataatgg
     6901 cgtcacattc tgtatgaccg ctgcactttg catttcctgc aatgctggca cggaaagtct
     6961 gtttggaggt tcccttagca acggaacggg atactacatc ggcactggag ccttcaccat
     7021 ttaactctac ctcatagata ctttcagcca cctgggcccc atgagtgaga agacgctctc
     7081 ttactaccag tctggcacct gctgccagtt tcgctgtatt ggtccggatg gtagagtcaa
     7141 cacccttgat ctggaccatc tccatttcca tctggctgtt ttcttccata tatacttctg
     7201 ttcccggatt cagaacacgt tttcctgtac cgtcaccttc accgtaatgc ttttctacgt
     7261 atttaacttt cgcattcttt ccaatgaaaa atctgtggat gccgtcatgc tcagagtcct
     7321 gatcgccaca gttgtggata ccacagcctg ctacgatcac tacatcagag tcttcgccaa
     7381 tgtagaaatc attgtaaacc atttctttta atccgctggc acttaccact accggaatat
     7441 ggacactttc atgctttgtt cccggcttga tcttaatatc aattcccggt ttatctgttt
     7501 tagttgtaat atcaatgttg gctgtggtac ttcttccagc cattttcccg ttagcacgga
     7561 tattgtaagc accttccggc actccgtgaa ggtcagctac ttctgcaaga agtctctcct
     7621 ggatctgatc catcattcct gatcctcctt tccgtcattt cccgtaaaac cggaacaaac
     7681 gcctactgct gctgcggttc ccataatttc aggcatgatc tcttctctgg ttccctgttt
     7741 tacgatcttg ccatctgcga ttacaacgat ctcatctgca atatttaaaa tacgctcctg
     7801 atgggaaata atgagaatgg agccattatt attctttctc atacgctcaa atacctggat
     7861 caggttctgg aagctccaaa gatcgatacc cgcttccggt tcgtcaaaaa cagaaagctt
     7921 tgcatttctt gcaataacag tagcgatctc aattctcttt aattcaccac cggaaagact
     7981 tgcgttaatc tcacggttga tataatcttt tgcacaaagt cctacctcgg acaaatactg
     8041 acaggcctgc gctgctgaca gcttctttcc tgatgccaga cgcagaagat ccaatacctg
     8101 aacaccttta aaacggactg gctgctggaa agcaaaactg atgcccatcc ttgctctttc
     8161 cgtaatgttc ttctctgtaa tatcttctcc gtcaaagaaa atctgaccgc cggtcgggcg
     8221 ttcgatcccc atgatcagct ttgctgtggt ggattttcca cctccgtttg gtcctgtaat
     8281 gaccacaaat ttgccatcat tcaccttcag gctgatatct ttgatgattc ctttttctcc
     8341 cttgtcatca tttacttcaa aggagagatg ttttagttcc agcatctgct gtccccccac
     8401 ctttctttat tattccaaat aatagttttc cgtttatctt ctccaaaata tccggaaagc
     8461 aaaaacagga agccagtttc ctgacctcct gctcatctcc tgcagaagaa gggagtcgaa
     8521 ccctcaaggt attactacca cacggacctg aaccgtgcgc gtctgccaat tccgccactt
     8581 ctgcatctct gcgttgctat tttctcaagc aacgtagatg attatatcgt aaaacatatt
     8641 gtttgtcaac agtaattttg aattttttga aaagtggaag gttttgtaaa aaatataaaa
     8701 tttaaaccac ctgctgaccg gtcagttcgt atcggctttt acaaagtttc gataaaccgt
     8761 ccgaaatcaa ttcttccctt ttttgtattc ttatatacac cggcgcattc caaaacctgg
     8821 acaaacactt ttccaatttc tttctgcaga atggcatgga tgttatcctc tgttatttca
     8881 taggatccaa gccaggaatc cacccagtct gcatgctttt cgatctgcgg attgctgcgg
     8941 acatcctgct tttcaaggat atattgttcc aaaagcgcca tttccttttt cagccgcgcc
     9001 ggcaaaacag ccagtcccat tacttcgatc aggccgatgt tttccttctt tatatggtga
     9061 aggtctgcag acggatgata taccccccac gggcactcat ccgttgtgac attgtttcga
     9121 agtaccagat ccagttcata ctgatttcca tgcattcttg cgatcggagt aatcgtattg
     9181 tgcggtgttc cgtctgtttc actaaaaatg aaagcttcct tatcggtata tctgcgccat
     9241 gcagtaagga tatgatctgc tgccagagtg atcctttctg tatcggttcc tcttaatcgg
     9301 ataacagaca tgggccatct cacgataccg gctgtcagat cctcatagcc cggaatgaca
     9361 aattcactct cataaggtgc cttggccatt gcaaagacat agccgccacc ctgaaaatga
     9421 tcatggctta aaatggaacc gccgacaatc ggaaggtccg cgttggagcc ggccgtatag
     9481 tgcggaaaca actttacaaa ttccaatagt tttctaaata cagcctggtc aatcttcatt
     9541 ggccggtgcg cttcattgaa aatgatgcag tgttcattgt agtaaacata cggag
//
LOCUS       DBMDUA010000106         9560 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3396_length_9560_cov_2.559044, whole genome shotgun sequence.
ACCESSION   DBMDUA010000106 DBMDUA010000000
VERSION     DBMDUA010000106.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9560)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9560
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3396_length_9560_cov_2.559044"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="ACZPCL_08725"
     CDS             complement(<1..210)
                     /locus_tag="ACZPCL_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1162585.1"
                     /translation="MNRKNKKLLKLIGIHVLAVAVVIIMIFPVYWLVVSALQSPQTVT
                     KIPPSVVPSTYSLYFVKKVFTEFGIA"
     gene            complement(226..1128)
                     /locus_tag="ACZPCL_08730"
     CDS             complement(226..1128)
                     /locus_tag="ACZPCL_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1162586.1"
                     /translation="MLGSGSKKRRAGVQGTLLYMPAILIIFGIVVYPMLYALVMSFTG
                     YSVRKPVMNFIGVANYIKILKDASFWQAVVRSLMFTFGSLIPQVVLGLAIAILLNHPD
                     LRCKGLFRGLVIMPWLVPTVAVAMIFRWMFHDVYGIMNYILMKLHILKEPVAWMANEH
                     TAMFILILANVWRGVPMLITMFLAGLQGIPYDLYEAGQIDGANGWNQFSKITPPLLMP
                     VVMVSGILRFIWTFNFYDLPWVMTGGGPAEATQTTPIYAYRRAFSSYRMGEGSAITMI
                     LFVLLIVFAAIYFMLKKRQEKMYN"
     gene            complement(1201..2601)
                     /locus_tag="ACZPCL_08735"
     CDS             complement(1201..2601)
                     /locus_tag="ACZPCL_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1162587.1"
                     /translation="MRKKMKKALGVILGATVMMAVAGCGQSNSSGGQSTQEAKTESSK
                     AEETKAEESKPEEEATGTIEMWSMLTQQERADELQKLADSYMEEHPGTQINITVMPWS
                     GAMDKIVSAIMAGNAPDIMITGTGYPQSLAGTGGLLELSDLVEEIGGADTFLGTSLSV
                     QGAYEDGLYSVPIYITPYVAYYRESWLKEAGIEKLPETWEEYYDMCKAVTDPSKDRYG
                     FALPLGDLHAWKTIWSFLQANDVDLVNVDDNGEWYVDLDDESRAAMVETYEYLYKLVK
                     DCAPEGTVGYTQTNVREMIAGGNVMSRIDTPEIYYTLQSVSPDDMDDVAYFKIPGRKK
                     TGSGQGWVGLSISADGNTGLAKDFIKYMFTGDKLVDFYLSYPYAMFPVREELYSDSKY
                     QENLPDNLKPMVPDMALDILSTSNGLTMVNGPFPGAGEFEAQCVLGNGLIKMLSDGYS
                     ADQAVDYIVGELEKLL"
     gene            complement(2620..3726)
                     /locus_tag="ACZPCL_08740"
     CDS             complement(2620..3726)
                     /locus_tag="ACZPCL_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR1162588.1"
                     /translation="MVITDIKINAYSATYTTPISNGKYTYPATEIVICEIMTDEGING
                     IGWVHGSDMVIKAILSLKERVIGQDPFNVERIWEKMYLPKVYGRKGLATRAISGIDIA
                     LWDIRGKVAGRSVCQMLGGYNDSIPAYIAGGYYEDGKGLDLLQNEMKENLKKGARAIK
                     MKIGGVSIREDLERIDAVRDAIGPDIHLLVDANNAYNRIDALKMGRELERRDIYWFEE
                     PLSPDDIEGCAELQRKIDVPIAIGENEYTRWGFKQLIEANAAQVLNADAQILGGITEW
                     KKVADLAMASHILIAPHGDQEVHSHLVASVPNGLIAEYYDNNTNALLKDMFSKPIKLN
                     HKGELAVSTAPGLGVEIEYDRIRRYCTYSSDNKQ"
     gene            3985..4500
                     /locus_tag="ACZPCL_08745"
     CDS             3985..4500
                     /locus_tag="ACZPCL_08745"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1162589.1"
                     /translation="MNYRTAQTADLPAMMKMVEEAKEGFRARNIDQWQKGEPNEQGLT
                     EGIEKSVVHVLEENGEVLGMITVVPGPEADYAVIDGAWLNDEPYFAFHRVCVSEAHKG
                     KGLAASLFSYSEAFAKEKGVRNIRIDTHPDNLSMQKALAKNGYTFCGKITLCSGSEAG
                     DPRFAYQKILN"
     gene            complement(4544..5410)
                     /locus_tag="ACZPCL_08750"
     CDS             complement(4544..5410)
                     /locus_tag="ACZPCL_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1162590.1"
                     /translation="MKVPFSVDLTGKTAIVTGGSGVLCSAMAYGLAVNGAKVAIIGRN
                     KEKLAKMSETLTENAKNEYSKEATVKGYSCNVLDKEELVKTYEQIKAALGSCDILING
                     AGGNQPGAIASVKMLKKEKQEDDYSFWDLKEENIRNVMDLNYMGTLLPIQVFTRDMVE
                     KRKGSIINIASVSSILPLSKVMAYSNAKSAVQSLTQWLAIHFGQSGIRCNAIVPGFYA
                     AEQNHDLLFNKDGSYTDRAGDIIAGTPMGRFGNPEELIGAALFLASDDAAGFVNGIVL
                     PVDGGYSAFSGV"
     gene            complement(5699..9472)
                     /locus_tag="ACZPCL_08755"
     CDS             complement(5699..9472)
                     /locus_tag="ACZPCL_08755"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR1162591.1"
                     /translation="MSVRRIFVEKKPDYAVKAGELREELVSYLGITGVKSVRVLIRYD
                     IENLSEDTYKKALGTIFSEPPVDEVYEEEFPKNPGDLVFSVEYLPGQFDQRADSAMQC
                     VKLLKEDEEPVIRTATTYVITAPLTLAQEDAIRHFCINPVDSRQAEEDKPKTLVTEFE
                     VPEDIKSFEGFAKASEEELKALYDTLNLAMTFKDFLYIQKYYAGEEHRDPTVTEIRVL
                     DTYWSDHCRHTTFSTELKNVTFTEGEYKKPIEDTYKKYLADRAVIYKDRKDKYVCLMD
                     LALMAMKKLRSEGKLEDQEESDEINACSIVVPVEIDGKTEEWLVNFKNETHNHPTEIE
                     PFGGAATCLGGAIRDPLSGRTYVYQAMRVTGAADPTKPLNETLKGKLPQRKIVTSAAA
                     GYSSYGNQIGLATGYVKELYHPGYVAKRMEIGAVMGAAPRKYVKRLTSDPGDIIILLG
                     GRTGRDGIGGATGSSKVHTTASIEVCGAEVQKGNAPTERKIQRMFRRPEVSSIIKKCN
                     DFGAGGVSVAIGELAAGLQIDLDKVPKKYAGLDGTEIAISESQERMAVVVDPADVEKF
                     LGYAAEENLEAVTVAVVTEDPRLVMNWRGKTIVDISRAFLDTNGAHQEADVTLEVPAH
                     EGNLFDKKEVGNVKDKWLNMLSDLNVCSQKGLVERFDGSIGAGSVFMPFGGKYQLTET
                     QSMVAKLPVLEGHTDTVTMMSYGFDPYLSSWSPYHGAVYAVVSSVAKIVAAGGDYKKI
                     RFTFQEYFRRMSSDPTRWSQPFAALLGAYNAQMGFGLPSIGGKDSMSGTFDDEHGEIN
                     VPPTLVSFAVDVNSCKNIITPEFKKAGSKIVEFKINRDNYDLPDYAQIMDGYGKLFED
                     IKAGKILSAYAVEGRGAAEAVSKMAFGNKMGVKIEHNVDPRDFFGAGWGNIVCEVAEG
                     KVGELSIPYTLLGEVTDKGVFEYGNTTITMDEALASWMKTLEDVFPTVTGKEKTGEAA
                     KIEEKLYDTDTIYICDHKLAQPEVFIPVFPGTNCEYDSTKAFERAGAKVVTKVFKNLS
                     AQDIRESVEEYKKEIAKAQIIMFPGGFSAGDEPDGSAKFFATVFRNAVIKEEVEKLLN
                     ERDGLMLGICNGFQALIKLGLVPEGKITEQKPNSPTLAMNTIGRHISKMVYTKVMTNK
                     SPWLAEAKLGGVYCNPASHGEGRFVADEAWLHRLIANGQVATQYVDDKGNVTMDEYWN
                     PNGSYMAIEGITSPDGRILGKMAHSERRGEAVAMNIYGEQDMKIFESGVKYFK"
BASE COUNT         2428 a         2398 c         1808 g         2926 t
ORIGIN      
        1 tgcaattcca aattctgtaa aaactttctt tacaaaataa agagaatacg tgcttggaac
       61 aacagacggc gggatttttg ttacagtctg agggctttgc agtgcagaaa ccaccagcca
      121 atataccgga aatatcataa tgatcactac tgccactgcc agaacatgga tcccgatcag
      181 tttcagaagc tttttatttt ttctgttcac acatccacct cccttttagt tatacatttt
      241 ctcctgccgt tttttcagca taaaataaat tgctgcaaat acgatcagaa gtacaaacag
      301 gatcatggtt attgcagatc cttctcccat cctgtaactg ctgaacgctc ttctgtaagc
      361 ataaatagga gttgtctgcg ttgcctctgc aggtccgcct ccggtcatga cccacggaag
      421 atcatagaaa ttaaatgtcc agataaatct caggatgccg gataccataa caaccggcat
      481 taaaagcggc ggtgtgatct tactaaactg attccatcca tttgcaccat cgatctgtcc
      541 tgcttcatac agatcataag gaattccctg aaggcctgcc agaaacattg taataagcat
      601 gggcacaccg cgccagacat tagccagtat caggataaac atagccgtat gttcattagc
      661 catccacgct acaggttctt ttaatatgtg cagtttcatt aaaatgtaat tcatgatccc
      721 ataaacatca tggaacatcc atctaaatat catcgccacc gctaccgttg gcaccagcca
      781 gggcatgatc accagacctc taaacaatcc cttacagcga agatccggat gatttaataa
      841 aatggcgatt gccagtccca ataccacctg cggaataagg gacccaaaag taaacataag
      901 tgatcttact actgcctgcc agaatgaagc atcttttaat atttttatat aattggctac
      961 acctataaaa ttcattaccg gttttctgac actgtatccc gtaaagctca ttacaagcgc
     1021 ataaagcata ggatacacta cgattccaaa tatgatcagg atcgccggca tataaagtaa
     1081 tgttccctga actcctgccc ttcttttctt acttccgcta ccgagcattc gcttatccac
     1141 ccctcttaga cagaatggat attctgccgt tcttcttcag aatatccatt cttaaaacaa
     1201 ttataataac ttctctaatt cacctacaat atagtctaca gcctggtctg cgctgtatcc
     1261 gtctgaaagc atcttgatca gtccatttcc aagaacgcac tgtgcttcaa actctcctgc
     1321 tcccgggaac ggtccattga ccatagtcag tccattggat gttgaaagaa tatctaatgc
     1381 catatccgga accattggtt tcaggttatc aggaaggttc tcctggtatt tgctgtcact
     1441 ataaagttct tctcgaaccg ggaacatggc atatggatag ctcagataga aatcaaccag
     1501 cttatcgcct gtaaacatat attttataaa gtcttttgcc agtcctgtgt taccatcagc
     1561 agaaatactc aaaccaaccc aaccctgtcc ggaaccggtc ttttttcttc caggaatctt
     1621 aaagtacgcc acatcatcca tatcatccgg agatacagac tgtaaggtgt aataaatttc
     1681 cggcgtatca atacggctca ttacattacc accggcaatc atctctctta cgtttgtctg
     1741 tgtatatcca acagttccct ccggtgcaca gtcttttact aacttataaa gatattcgta
     1801 ggtttctacc atagcagccc ggctctcatc gtccaggtcc acataccatt cgccattgtc
     1861 atctacgttt acaagatcaa catcgtttgc ctgaagaaaa ctccagatgg tcttccatgc
     1921 atgcaggtca cccagaggaa gagcaaaacc atatctgtcc ttagaaggat ctgttacagc
     1981 cttacacatg tcataatact cttcccatgt ttccggcaat ttttcaattc ctgcttcttt
     2041 cagccagctt tctctgtaat aagctacata aggggtaatg taaataggta cggaatacag
     2101 tccatcttca tatgcaccct gtacgcttaa agaagttccc aaaaatgtat ccgctccgcc
     2161 tatttcttca actaaatctg ataattccag caaaccacct gtacctgcta aagactgggg
     2221 atatccggtt cctgtgatca tgatatccgg cgcatttcca gccatgatcg cggaaacgat
     2281 cttatccata gcgccactcc acggcataac cgtgatattg atctgtgttc caggatgttc
     2341 ttccatatag gagtctgcca gtttctgtaa ttcgtctgct ctctcctgct gagtcagcat
     2401 agaccacatt tcgatagttc cggttgcttc ctcttccggc ttactttctt ctgcttttgt
     2461 ttcctctgct tttgaagatt ccgtttttgc ttcctgtgtg ctctgtccac cggaactatt
     2521 gctctgtccg catcccgcta ctgccatcat cacagttgct cccagaataa ctcccagcgc
     2581 ttttttcatc ttctttctca tctttggacc tcccttattt tattgtttat tatccgaaga
     2641 atacgtacaa taacggcgta tcctatcata ttcgatctct actcctaacc ccggcgccgt
     2701 tgaaacagcc aattctccct tgtggtttag tttaatcggt tttgaaaaca tgtcttttaa
     2761 cagtgcattg gtattattgt cataatattc tgcgatcaga ccattgggaa cagaggctac
     2821 cagatgggaa tgtacctcct gatctccatg gggagctatt aaaatgtgag atgccatagc
     2881 aagatctgct acttttttcc attctgtaat gccgcctaat atctgggcat ctgcatttaa
     2941 cacctgagct gcatttgctt ctatcagctg cttaaatccc caacgggtat attcattttc
     3001 tccgattgca atgggaacat cgatctttct ctgaagttcc gcacagccct caatatcatc
     3061 aggtgataat ggttcctcaa accagtatat gtccctgcgt tccagttctc ttcccatttt
     3121 cagggcatca atccggttgt atgcattatt ggcatctacc agtaaatgta tatccggtcc
     3181 aatggcatct cttactgcat caatgcgttc cagatcttcc cggatcgata cacctcctat
     3241 tttcattttg atcgcccttg ctcctttttt cagattttcc ttcatctcat tttggagcag
     3301 atccagacct tttccatctt cataatagcc gcctgctata tatgccggta tgctgtcatt
     3361 atatcctcca agcatctgac agacgcttct tcctgccact ttgcctctta tatcccagag
     3421 ggctatgtca atcccactga ttgcccgtgt agcaaggcct tttctgccgt acacttttgg
     3481 aagatacatt ttttcccata tccgttctac attaaaagga tcctgtccga tcacccgttc
     3541 ctttaaactc aatattgcct ttatgaccat atctgagcca tggacccagc caattccatt
     3601 tataccttca tctgtcatga tctcacaaat cacaatctct gttgccggat atgtgtattt
     3661 accattgctg ataggtgttg tatatgtagc agagtacgcg tttattttaa tatctgtaat
     3721 taccatcttc tcggccctcc atcgtgtgtt gtgcattttg tatgcttttt atttgttttt
     3781 aatttttcta ttgatactat agcactattt ttagttttat gtcaatatct tttataaaat
     3841 ttatttacaa aattagtttt aaaatgtggt aattttttaa cgcatttatt atagtctgct
     3901 ggttacgatt gcaaaccgat ctgttcctca gtataataaa agcaaatatc aggagtaatt
     3961 tactatttta caggagggat ttttatgaat tacagaaccg cccaaacagc agacctgcct
     4021 gccatgatga aaatggtaga agaagccaaa gaaggatttc gggctagaaa tattgaccag
     4081 tggcaaaagg gagaaccaaa cgaacagggg cttacagaag gcattgaaaa aagcgttgta
     4141 catgtcctgg aagaaaatgg agaagtttta ggcatgatca ccgttgtgcc cggtcctgaa
     4201 gccgactacg ctgttatcga cggcgcctgg ctgaatgacg agccttattt tgctttccac
     4261 agagtctgcg tatccgaggc acacaaagga aaaggactgg ctgcaagcct cttctcttat
     4321 tctgaggcat ttgcaaaaga aaagggtgtc agaaacattc gtatcgacac ccatccggac
     4381 aatctctcca tgcagaaagc attagccaaa aatggctata ctttctgcgg aaagatcaca
     4441 ttatgttccg gaagcgaagc cggagatccc cggttcgcct accagaaaat tttaaattaa
     4501 tgcatatagc gttggtttga cctgagattg gtcatgagcc caattatacc ccgctgaaag
     4561 cgctgtaacc gccgtctaca ggcagtacaa tgccatttac aaagccggct gcatcatcac
     4621 ttgccaaaaa cagggctgcg ccgatcagct cctctggatt gccaaaacgt cccataggag
     4681 ttccggcaat gatgtctccg gctctgtctg tgtaggagcc atccttgtta aataacagat
     4741 catggttctg ctctgctgca taaaatcccg gtacaatggc attgcagcgg atgccactct
     4801 gtccaaagtg gatcgccagc cactgagtca ggctctggac agcgcttttt gcattggaat
     4861 aagccataac cttggaaaga ggaaggatag aggaaacaga agcgatattg ataatgcttc
     4921 cctttctctt ttccaccata tctctggtaa atacctggat aggcagtaag gttcccatgt
     4981 aattcagatc cattacattt ctgatattct cttccttcag atcccagaaa gaataatcgt
     5041 cctcctgttt ttccttcttt aacattttta cagaggcaat ggctcccggc tggtttcccc
     5101 ccgcgccgtt aatcaggata tcgcagcttc ccagcgctgc tttgatctgc tcataagtct
     5161 ttaccagttc ttctttatcc agtacattgc agctgtagcc ttttactgtt gcttctttgc
     5221 tgtattcatt tttggcattt tctgtaaggg tctcagacat tttcgccagt ttttccttat
     5281 tgcgcccgat aatggcaact ttcgccccgt tcacagccag accatatgcc atagcagaac
     5341 aaagcacacc gcttcctcct gttacaatgg ctgtttttcc tgtaagatcc acactaaaag
     5401 gtactttcat cgcttctcct ctttccgcgc cctttagggc actgttttat atttactgat
     5461 acatgaaaca tatgtaggta acatgtatga tcatcctgca tcggcatacc ggtataccgg
     5521 acatatatat caatatataa tataaaatat gcttcccgtc aactggtatt ctaaacttta
     5581 ttttattaaa ttttccccct tttcatcaat acaaaaatcc cggaagataa tccgaatttc
     5641 tcaagaccgt cttccgggac attttacaat attacactac ctgtgtcata gcttactctt
     5701 acttaaagta cttcacacca gactcaaaga tcttcatatc ctgttcgccg taaatgttca
     5761 tggcaactgc ctcgcctctt ctctcagagt gagccatctt accaaggata cggccatccg
     5821 ggcttgtgat accttcgatt gccatgtagg aaccgtttgg attccagtat tcgtccatgg
     5881 tcacattgcc cttatcgtct acatactggg tcgctacctg gccatttgcg atcaggcggt
     5941 ggagccatgc ttcgtctgct acgaaacgtc cttcaccgtg ggaagccgga ttgcagtata
     6001 caccacccag ttttgcttct gccagccatg gggacttatt tgtcataacc ttggtgtaaa
     6061 ccatcttgga aatatggcgt ccaatggtgt tcatagccag tgtaggagaa tttggcttct
     6121 gctctgtgat ctttccttcc ggaacaaggc ctaacttgat cagggcctgg aaaccgttgc
     6181 agataccaag cataagtccg tcacgttcat ttaacagctt ttctacctct tccttgatca
     6241 ctgcattgcg gaatacagtt gcaaagaatt tagcggaacc gtctggctca tcaccagcgg
     6301 agaagccgcc tgggaacatg atgatctgtg cctttgcgat ctctttcttg tattcttcta
     6361 cagactcacg gatatcctgt gcacttaagt tcttaaatac ctttgttact acctttgcgc
     6421 ctgcacgttc aaaggctttt gtgctgtcgt actcgcagtt agtaccaggg aatacaggga
     6481 taaatacttc cggctgtgcc agcttgtggt cgcagatgta aatagtatct gtatcgtaaa
     6541 gcttttcttc tatctttgca gcttcgcctg tcttttcttt gcctgtaaca gttgggaata
     6601 cgtcctctaa ggtcttcatc cagcttgcta aagcttcatc catggtaatg gtggtatttc
     6661 catattcaaa tacacccttg tctgttactt cgccaagtaa agtatatggg atggaaagtt
     6721 ctcccacttt tccttctgct acttcgcata cgatattgcc ccagcctgcg ccaaagaaat
     6781 ctcttggatc tacattgtgc tcgatcttaa cgcccatctt gttgccgaat gccatcttgc
     6841 ttacagcttc cgcagcgcct ctgccttcta ctgcatatgc agaaaggatc tttccagcct
     6901 tgatatcttc aaacagcttt ccgtagccgt ccatgatctg ggcgtaatcc ggcagatcat
     6961 agttatcacg gttgatctta aattctacga tcttgcttcc tgctttctta aattcaggag
     7021 taatgatgtt cttgcagctg tttacatcta ctgcaaagga aacaagtgtc ggaggtacat
     7081 tgatctcacc gtgttcatca tcaaaggtac cggacatact gtccttaccg ccgatggaag
     7141 gaagtccaaa gcccatctgc gcattgtaag caccaaggag tgctgcaaat ggctggctcc
     7201 atctggttgg gtcgctgctc attctgcgga agtattcctg gaaagtaaag cggatctttt
     7261 tataatcacc gcctgcagcc acgatctttg ctacagaaga gacaactgca taaacagcgc
     7321 catggtatgg gctccagctg gaaagatatg gatcaaagcc gtagctcatc atggtaacgg
     7381 tatctgtatg tccttctaat acaggaagct ttgctaccat ggactgtgtt tctgtcagct
     7441 gatatttgcc gccaaatggc ataaatacag aacctgcgcc aatagaaccg tcaaaacgct
     7501 ctaccagtcc cttctgtgag cacacattta agtcggacag catgttcagc catttatctt
     7561 ttacatttcc tacttctttc ttatcaaaaa gatttccctc atgtgcagga acctctaagg
     7621 taacatctgc ttcctgatga gcgccgtttg tatcaaggaa agcacggctg atatctacaa
     7681 tggtctttcc tctccagttc atcaccagtc ttggatcttc agttacaact gcaacggtaa
     7741 ctgcttccag gttttcttct gctgcatagc ctaagaattt ctctacgtca gcaggatcaa
     7801 ctacaactgc catacgctcc tgggactcgg aaatagcgat ctcagtaccg tcaagacctg
     7861 catatttctt cggaaccttg tccaggtcga tctgaagacc tgctgccagc tcaccgattg
     7921 caacagatac accgcctgca ccaaagtcat tacatttttt aataatggag cttacttccg
     7981 gacgacggaa cattctctgg atcttacgct cggtaggtgc atttcctttc tgtacctctg
     8041 caccgcatac ctcaatagaa gcagtggtgt gtaccttgga agaaccggta gcacctccaa
     8101 taccgtcacg gccggtacga ccgcctaaaa ggataatgat atctcctgga tcagaggtta
     8161 aacgctttac atattttctt ggagcagcgc ccataacagc accgatctcc attctcttag
     8221 ctacataacc tggatgatat aattctttta cataaccggt tgccagaccg atctggtttc
     8281 cataagaaga atatccggca gctgcgctgg taacgatctt tctctgagga agtttgccct
     8341 ttaaagtctc atttaatggc tttgtaggat ctgctgcacc ggtaacacgc attgcctgat
     8401 atacataagt acggccggat aacggatcac ggatggcacc gcctaagcaa gtagccgcgc
     8461 cgccgaaagg ctcgatctct gttgggtggt tgtgagtctc atttttaaaa tttaccagcc
     8521 actcttctgt ctttccgtcg atctcaacag gaactacgat gctgcaggca ttgatctcgt
     8581 cagactcttc ctggtcttct aacttgcctt cgctgcgcag cttcttcatt gccataagag
     8641 ccaggtccat caggcataca tatttgtcct tgcggtcctt gtagattact gcacggtcag
     8701 ccaggtattt cttataagta tcttcaattg gcttcttgta ttcgccctct gtaaaggtta
     8761 catttttaag ctctgtggag aaggtggtat gacggcagtg gtcagaccag taggtatcta
     8821 acacgcggat ctcagttaca gttggatctc tgtgttcttc tcctgcataa tatttctgta
     8881 tatataagaa gtctttgaat gtcattgcaa gatttaaggt atcatataat gcctttaatt
     8941 cttcttcaga agcctttgca aagccttcaa agctcttaat atcttccgga acctcaaact
     9001 cagttaccag agtctttggc ttgtcctctt ctgcctgacg gctgtcaacc gggttgatgc
     9061 agaaatggcg gatggcatct tcctgtgcca gagtcagcgg tgctgtgatc acataggtag
     9121 ttgctgtacg gatcaccggt tcctcatctt cttttaacag cttgacacac tgcatggcag
     9181 agtctgctct ctgatcaaac tgtcccggca ggtactcgac ggaaaatacc agatctcccg
     9241 ggttctttgg aaattcttcc tcataaacct catctacagg cggctctgag aaaatggttc
     9301 ccagtgcttt cttataggta tcttctgaaa gattttcaat atcataacgg atcagtaccc
     9361 gtacactctt cactccggta atgccaaggt agcttactaa ctcttctctt aactctcctg
     9421 cttttaccgc ataatcaggc tttttctcta caaaaattcg tcttacgctc attacagacc
     9481 tcctgttttt catagctttt agcttcttct gtgccggacc attatataaa tggcctcgtt
     9541 tgcctgtttt actttaactt
//
LOCUS       DBMDUA010000107         9336 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3463_length_9336_cov_2.001516, whole genome shotgun sequence.
ACCESSION   DBMDUA010000107 DBMDUA010000000
VERSION     DBMDUA010000107.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9336)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9336)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9336
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3463_length_9336_cov_2.001516"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="ACZPCL_08760"
     CDS             complement(<1..366)
                     /locus_tag="ACZPCL_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162592.1"
                     /translation="MEFGVWSLVPPVLTIALALTTKNVFISLLIGILTGNLVLNHFAV
                     FTGLNAGIYSILDTMTESNTLIIGSVLITAAVIHLAEKSGGIEGFVNVVVKKQGLIKS
                     KRGANLFTWLLGLVVFTSGT"
     gene            complement(485..1837)
                     /locus_tag="ACZPCL_08765"
     CDS             complement(485..1837)
                     /locus_tag="ACZPCL_08765"
                     /inference="COORDINATES: protein motif:HMM:NF019581.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1162593.1"
                     /translation="MKTLIKNGLLIDPANQVQAYLNLILEDGKVVAVTEEEPEADRVI
                     DATGKVVAPGFIDIHMHEDPVKDGKIEFCIFDAMLRMGVTTAVGGNCGINVYDPVKYL
                     DMVEKDGAPVNVMLFAGHQYFREAAGALDKYAPVTKEQQEHMKKGLEKALEAGCVGIS
                     YGLRYVPGTGRDEFLDTASCCEKEHRMISAHVRDDEDRVFGAVAEVAEAGKLYNIPVQ
                     VSHIGSMAGFGQMKQLLRQVDGYRMNGIDVACDCYPYFAFSTRIGATTYDDGWLERYH
                     CDYSACQLTEGKYKGQRCTPETFAEMRRDFPECLTVCYVMDENDIRMAFADPGVMVGS
                     DGLIDNGHGHPRAAGTFPRFLSEFVRKGDISLYDGIEKMTAAPAKRLGLSNKGRLNVG
                     ADADVVIFDMEKIQDGANFDDPMIPPTGIDYVLIGGEVAAKDCKIVKGDLGTALRKLS
                     "
     gene            complement(1910..2965)
                     /locus_tag="ACZPCL_08770"
     CDS             complement(1910..2965)
                     /locus_tag="ACZPCL_08770"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HIR1162594.1"
                     /translation="MSQILEQAEKLLQALPKEQLGFFPTPFYRLDRLSEELGVNLFIK
                     RDDFTGMNLFGGNKVRKLEYLLGEAKAKGCEYAITYGATQSNHAMETAAACRRCGIKP
                     ILYLTAVVEPDDADVRANLLLDKILDVELHIVDILPGETEDDAEARSFEMGAARAKEL
                     NESGHPCYDIPMGGASVVGSVGFANGYVEFEKQVKAMNLNVDYIFHATGTGGTMAGLA
                     AGRKMAESDTKIISITVSPKDEKYLRKVEKLANDVLALIKSPAHVTIEEDLHMDTGYY
                     APGYEQPNEAASEAIRLLARTEGLFVDPVYTGKALAGLIDYVRSGRVPQGSNVVFWHT
                     GGATALFAEHEILGKLY"
     gene            complement(3229..4146)
                     /gene="metA"
                     /locus_tag="ACZPCL_08775"
     CDS             complement(3229..4146)
                     /gene="metA"
                     /locus_tag="ACZPCL_08775"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase MetA"
                     /protein_id="HIR1162595.1"
                     /translation="MPIKVQRDLPARAILESENIFIMDEDRAMSQDIRPLEILILNLM
                     PLKEDTETQLLRALSNTPLQVDCTFLMLSTHVSKNTSASHLNKFYVSFDSIKKKKFDG
                     MIITGAPVENMEFEEVNYWEELSGIMEWTKTHVTSTIHICWGAQAGLYYHYGIPKYKR
                     AEKLSGIYNHRVLDRKVPLVRSFNDTFLAPHSRYTEVRREDIEKHPELVILAESEEAG
                     VFLVMSRDGKQIFVQGHPEYDRMTLNNEYHRDLNKGLNPSLPVNYYVDNDPFSVPELT
                     WRNTSNTLYTNWLNFYVYQMTPYLLDDGE"
     gene            complement(4148..6124)
                     /gene="ligA"
                     /locus_tag="ACZPCL_08780"
     CDS             complement(4148..6124)
                     /gene="ligA"
                     /locus_tag="ACZPCL_08780"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR1162596.1"
                     /translation="METEIQRMKELIPQLRQAAKAYYQESREIMSNFEYDKLYDELSA
                     LEAKTGIILSGSPTQQVGYQVLSELPKETHEEPMLSLDKTKSVEDLQQWLGSQKGLLS
                     WKMDGLTVVLTYENGTLAKAVTRGNGEIGEVITPNARAFSNIPLTIAYKGQLIIRGEA
                     VITYTDFERINEEIGDADAKYKNPRNLCSGSVRQLNSKITAERSVHFEAFALVKAEGV
                     DFKNSRKEQFLWLKNQGFEVVHHEEVTAADLPDKVAWFAEQIEHNDIPSDGLVLLYDD
                     IAYGDSLGRTAKFPRNAIAFKWQDEIRETTLSYIEWSPSRTGLINPVAVFEPVELEGT
                     TVSRASVHNISIMEGLKLGVGDKITVYKANMIIPQIADNLTRSGVRDIPKICPVCGGA
                     TEIRQTNDVKSLYCTNPECQAKKIKSFTLFVSRDALNIDGLSEATLEKFIGAGFIHEY
                     ADIFHLSGHKEVITEMEGFGQKSYDNLISSIETASHTTLPRLIYGLGIAGIGLANAKM
                     LCRHFNYDLDQMRHCNVEELTAVDGIGGVLAQAWVDYFASEKNNEVLDHLLQELHIEK
                     EQTEENGTAFAGMTFVITGSVEHFANRKELQALIESKGGKATGSVTSKTTYLINNDTQ
                     SSSSKNKKAKELGIPILSEEDFLNLLQEKEKGEG"
     gene            complement(6100..6936)
                     /locus_tag="ACZPCL_08785"
     CDS             complement(6100..6936)
                     /locus_tag="ACZPCL_08785"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009533047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR1162597.1"
                     /translation="MEERLNKWLSRMGACSRREADRLIQEGKVLIDGKVAQMGQRVED
                     GQKVICDGKPVGDTDGGRGEVKPPRPVLLAVNKPRGIVCTTSDKDRAENIVEMLSYPE
                     RIYPIGRLDKESEGLILMTNQGDLVNKIMRSTNAHEKEYIVTVDKVLTDEFIKQMSEG
                     VWLEELERKTLPCKVRKQGERRFSIILTQGLNRQIRRMCQACGYRVHLLRRVRIMNIQ
                     LGGLKTGEYRKVTGEEYKRLIKLLEGSTSLSEADRRGKRYVAFHPRKEDQVNKWKQRS
                     RE"
     gene            complement(6945..7997)
                     /gene="queA"
                     /locus_tag="ACZPCL_08790"
     CDS             complement(6945..7997)
                     /gene="queA"
                     /locus_tag="ACZPCL_08790"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR1162598.1"
                     /translation="MDVKDFYFDLPQELIAQDPLEDRSSSRLLVLDKKTGKTQHRIFK
                     DIVEYLRPGDCLVINDTKVIPARLFGVKKDTGAKIEVLLLKRRENDIWETLVKPGKKA
                     KPGTVIDFGDGILTGTVIETVDDGNRLIQFSYEGIFEEILDRLGQMPLPPYITHQLKD
                     KNRYQTVYAVHEGSAAAPTAGLHFTKELLKQIEDMGVKIAHVTLHVGLGTFRPVKVEN
                     VLDHHMHSEFYVVEEAEAKKVNDTKAAGGRVICVGTTSCRTVESASTEDGILQAKTGW
                     TDIFIYPGYKFKILDALITNFHLPESTLVMLVSALAGRENILAAYNEAIREKYRFFSF
                     GDAMFIADDPQPVKKA"
     gene            complement(8074..9045)
                     /locus_tag="ACZPCL_08795"
     CDS             complement(8074..9045)
                     /locus_tag="ACZPCL_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007035944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162599.1"
                     /translation="MRHSNRGRRKSGKAFDDKTKYARMLAIPAAIVLVLLVVILVMDR
                     KPAGDKAQGSSTSAEVTNASDISIENDTETGTDDGSVEPDNNEYHQDFSAYELQKDAY
                     PQVNELISTYFQAKVDQDVQLLYKVFGKEEDDRLEERKQQLKDEAVYIEDYQDITCYT
                     KAGMTDDSYVVYVTYDVKFRRVDTLAPGLMWCYVVKNDNGDYIIRENVVGDEADYVAN
                     QNQTEDVRLLSTQVNERLKQAVESDSLLAGIYKDLSNGAVVSSSEEGADSQVMIGDGA
                     DASSDAGEGNADESSAAGETSAEESQEAQTEAPESAGESSDAAVAIQ"
BASE COUNT         2271 a         2391 c         1813 g         2861 t
ORIGIN      
        1 ggtcccggaa gtaaatacca ccagacctaa cagccaggtg aaaaggttcg cccctctttt
       61 agacttgatg agcccctgtt tctttacaac aacatttacg aagccctcaa taccgccgga
      121 cttctctgcc agatggatca ctgctgctgt gatcagtaca gagccgatga tcagggtgtt
      181 gctctctgtc atggtatcca ggatgctgta aatacctgca tttaagcctg taaatactgc
      241 aaaatggttc aataccaagt ttcctgtaag gataccgatc aaaagtgaga taaatacgtt
      301 tttcgttgtc agtgccagcg caatggtaag gactggcggc acaagtgacc atacaccaaa
      361 ttccatactg ttttcccctt tcacttttcc tttttgtatt atgtactttt gtattttcag
      421 tcgccttttt cagacgtttc tgaccatatt tttaaaaatc ttatgacact ttctatcata
      481 ctttttatga cagctttctc agtgcagttc ccagatctcc ctttacgatc ttgcagtctt
      541 ttgctgccac ttcaccaccg atcagcacat agtcaatacc tgttggtggg atcataggat
      601 cgtcaaagtt tgctccgtcc tggatctttt ccatatcaaa gataactaca tctgcgtcag
      661 cgcctacatt taagcggccc ttgttagaaa gtcccagacg ttttgcagga gctgctgtca
      721 ttttttcaat tccgtcatac agagaaatgt ccccttttct tacaaactct gaaaggaatc
      781 ttgggaaagt accggctgct cttggatggc cgtggccatt gtcgatgagg ccgtcgcttc
      841 ctaccataac gccaggatct gcaaatgcca ttcggatgtc attttcatcc attacatagc
      901 ataccgtcag gcactccggg aagtcacggc gcatttccgc aaaggtctcc ggtgtacatc
      961 tctgtccttt atactttcct tctgtcagct ggcatgcact gtaatcacag tgataacgct
     1021 ccagccagcc atcatcatag gtagttgcgc caattctggt ggaaaatgca aagtatggat
     1081 agcagtcgca ggcaacatca ataccgttca tgcggtaacc atctacctgg cgcagcagct
     1141 gtttcatctg tccgaaaccg gccatgctgc cgatatggga tacctgcacc ggaatgttgt
     1201 ataattttcc tgcctctgcc acttctgcta ctgctccaaa cactctgtcc tcgtcatctc
     1261 ttacatgagc ggagatcatt ctgtgttcct tttcacagca ggaagcagta tctaagaatt
     1321 cgtcacggcc tgttcccgga acatagcgaa gtccgtagga aattcccaca cagcctgctt
     1381 caagggcttt ttcaaggcct tttttcatat gttcctgctg ctcttttgta acaggtgcat
     1441 atttatccag ggcgcctgct gcctctctga aatactgatg gcctgcaaaa agcattacat
     1501 tcactggtgc gccgtctttt tctaccatat ccagatattt caccggatca tacacattga
     1561 taccgcagtt tccgcctact gctgtggtaa cgcccatacg cagcatggca tcaaagatac
     1621 agaattcaat ttttccgtct tttaccgggt cctcatgcat atgaatgtcg ataaaaccag
     1681 gagccactac ttttcctgtg gcatcaataa ccctgtcagc ctcaggttct tcttctgtta
     1741 cagccactac ctttccgtct tccagtatca ggtttaaata agcctgtacc tggttggccg
     1801 gatcgatgag aaggccgttt ttgatcagtg ttttcacgac cttatccctc cttttatcta
     1861 ctcacaaatc ccgcacttac gtgctctact gcctgcttac gcaggcgatt tagtataact
     1921 tacctaaaat ctcatgctct gcaaacaggg cagttgctcc gccggtgtgc cagaatacta
     1981 cattgcttcc ctgaggaact cttccgcttc ttacatagtc gataagaccg gccaatgcct
     2041 ttcctgtgta tacaggatcc acaaacaggc cttctgtgcg tgctaacagg cggatggctt
     2101 cgctggctgc ctcgtttggc tgctcatagc ctggtgcata gtagccggta tccatatgaa
     2161 ggtcttcttc tatggttaca tgggccgggc tctttatcag tgccagcaca tcatttgcca
     2221 gtttttctac tttgcgtaaa tacttctcat ccttagggct tacagtaatg gagatgatct
     2281 ttgtatcgct ttctgccatc tttcttcctg ctgccagacc ggccatggtg ccgcctgttc
     2341 ctgttgcgtg gaagatgtaa tctacattca ggttcatggc ctttacctgt ttttcaaact
     2401 ctacataacc atttgcaaaa ccaacagaac ctacaacact tgctccgccc attgggatat
     2461 catagcatgg atggccggac tcatttaatt cctttgctct tgcagcgccc atttcaaagg
     2521 aacgtgcttc tgcgtcatcc tctgtttctc ccggaaggat gtctacaata tgaagctcta
     2581 catccaggat cttgtctaac agcagatttg cccttacatc cgcatcatcc ggctctacta
     2641 ctgcagtcag ataaaggata ggtttgatcc cgcatctgcg gcaggctgct gctgtctcca
     2701 tagcatggtt ggactgggta gcgccgtagg taatggcata ctcacagcct tttgcctttg
     2761 cttctcctaa aaggtattcc agtttgcgga ccttatttcc tccaaacagg ttcattcccg
     2821 taaaatcatc tctcttgata aacaggttga cgcctaattc ttcagaaaga cggtccagac
     2881 ggtaaaatgg ggtcgggaaa aatcccagct gttccttagg cagagcctgt aaaagttttt
     2941 ctgcctgttc cagtatctgt gacatgatta ctctcctctc cgcatttacc ctcaaactca
     3001 ataggtaaat gtttattttt ctacaactat agcacttttg tgtatatttg tacataaaaa
     3061 atgcagccac ggcgcagatt tcttacgatt tctgtgtcat gtctgcatat attgttcttt
     3121 ttctttccaa gacccaagac agcctatcac ccactgacgc aggtgaagaa cacctgtgtg
     3181 aagagcataa actgccattc ccgtcggtac aggaaccaga ccatcacatt actccccatc
     3241 atccagcaga tacggtgtca tctgatatac atagaagttc agccagtttg tatacaatgt
     3301 attggaagta tttctccatg taagctccgg tacagagaac ggatcattat ctacataata
     3361 attgaccgga agtgaggggt tcagtccctt attcagatct ctgtgatatt cgttatttaa
     3421 agtcatgcgg tcatattccg ggtggccctg gacgaagatc tgctttccgt cacggctcat
     3481 cacaagaaat actcctgcct cttcggactc tgccagaatg accagctccg gatgcttctc
     3541 aatatcttcc ctgcgcactt ctgtgtaacg ggaatgaggt gccaggaacg tatcattaaa
     3601 acttcgcact aatggtactt tccggtctaa taccctgtgg ttataaatac cggacagttt
     3661 ttccgccctc ttatatttcg ggataccata gtggtaatat aagcccgcct gggcgcccca
     3721 gcagatatgg atggtagatg ttacatgggt cttggtccac tccatgatac cggaaagctc
     3781 ttcccagtag tttacctctt caaattccat attttcaacc ggtgcaccgg taatgatcat
     3841 tccatcgaat tttttcttct taatgctgtc aaaggaaaca taaaacttgt ttaaatgact
     3901 ggcagatgtg tttttggaca catgggtaga cagcattaaa aaggtacagt ccacctgcag
     3961 cggtgtattg gacagcgcac gaagcagctg tgtttctgta tcttctttta aaggcatcag
     4021 gtttaagatc aggatctcca gcggtcggat atcctggctc atagccctgt cttcatccat
     4081 aataaatata ttttctgatt ccaggatcgc ccttgcaggc aggtccctct gtactttgat
     4141 cggcatatca gccttccccc ttttccttct cctgaagcaa attcaggaaa tcttcttccg
     4201 aaaggatcgg aattcccagt tctttcgcct ttttgttctt ggaagaactg gactgtgtat
     4261 cattgttgat cagataagtg gtctttgagg taacagaacc cgttgccttt ccacccttac
     4321 tctcgatcag tgcctgtaat tcttttctgt ttgcaaaatg ctctacagaa ccggtaataa
     4381 caaaggtcat tcctgcaaag gcagtaccat tttcctcagt ctgctctttt tcaatatgca
     4441 gctcctgtaa taaatgatct aatacctcat tgttcttttc agaagcaaaa tagtctaccc
     4501 aggcctgagc cagcacaccg ccaataccat ctacagctgt cagctcttcc acgttgcaat
     4561 gacgcatctg atccaggtca taattaaaat gacggcagag cattttggca tttgcaagac
     4621 cgatcccggc gatccccagt ccataaatca atctcggaag tgtggtatgg gaggccgttt
     4681 caatagaact gatcaggtta tcataggatt tctgaccaaa gccttccatt tctgtgatca
     4741 cttctttatg gccggataaa tggaaaatat ccgcatattc atggataaaa ccggcaccaa
     4801 tgaatttctc cagtgttgcc tcggaaagac catcaatatt taaagcatca cggcttacaa
     4861 acaaggtaaa gcttttgatc ttctttgcct gacattctgg attggtgcaa tataaactct
     4921 ttacatcatt ggtctggcgg atctccgtag caccgccgca caccggacag atcttaggga
     4981 tatcccttac gccgctgcgg gtcaggttat ctgcgatctg tgggatgatc atatttgcct
     5041 tgtatacagt gatcttatct cccacgccta acttaaggcc ttccataata ctgatattat
     5101 ggacactggc gcggctgacg gtcgttccct ctaactcaac cggctcaaat acagctaccg
     5161 ggttgatcag accggttctt gaaggactcc attcaatata agataatgta gtctcccgga
     5221 tctcatcctg ccatttaaag gcaatggcat ttcttgggaa cttggctgta cgtcctaaag
     5281 aatcaccata ggcaatatca tcgtataaca gaaccaaacc gtctgaaggg atatcattgt
     5341 gctcgatctg ctctgcaaac caggctactt tatccggcag atcagctgcc gttacctctt
     5401 catggtgtac tacttcaaag ccctgatttt tcagccataa aaactgttct tttctggaat
     5461 ttttaaaatc aacgccttct gccttaacaa gggcaaaggc ctcaaaatga acgctgcgct
     5521 ctgcggtgat cttgctgttt aactggcgca cagaaccgct gcaaagattt cgcggatttt
     5581 tatatttggc atctgcatct ccgatttctt cattgatgcg ctcaaaatcc gtataggtaa
     5641 tgaccgcttc ccctcggata atcagctgtc ccttataggc aatagtcaga gggatattgg
     5701 aaaatgccct tgcatttgga gtgataactt ccccgatctc cccatttcct ctggtaacag
     5761 cctttgcaag tgttccattt tcataagtaa ggactacagt cagaccatcc attttccagg
     5821 acaaaaggcc tttctggctt cctaaccact gctgtaagtc ctctacactt tttgtcttgt
     5881 caagagacag cattggctct tcgtgagtct cttttggaag ctcgctaagg acctgataac
     5941 caacctgctg ggtagggctt cctgacagga tgatccctgt ttttgcctcc aaggcagaca
     6001 gctcatcata cagcttatca tattcaaagt tgctcatgat ctcccggctc tcctgataat
     6061 aggcttttgc agcctgacgg agctggggga tcagttcttt cattctctgg atctctgttt
     6121 ccatttattc acctgatctt cttttctagg gtggaaagcc acatatcgtt ttcctcttct
     6181 gtctgcctca gacagacttg tggaaccttc caacagtttt ataagtcgtt tgtattcttc
     6241 cccggtgacc ttacgatatt ctcctgtttt caaaccgcct aactggatat tcataatgcg
     6301 caccctgcga agaagatgta cccggtatcc gcaggcctgg cacattcgcc ggatctgccg
     6361 gtttaagccc tgggtcagga taatcgaaaa acgacgttct ccctgtttgc ggactttaca
     6421 gggcagagtt ttccgctcta attcctctaa ccatacgcct tctgacattt gtttgataaa
     6481 ttcatcggtc agaaccttgt ccacggttac gatatattcc ttttcatggg cattggtact
     6541 gcgcatgatc ttatttacca gatccccctg gttcgtcata aggatcagtc cctcagactc
     6601 tttatccaaa cggccaatcg ggtaaatacg ctccggataa gaaagcattt ccacaatatt
     6661 ttctgcccgg tctttgtcag aagtggtgca gacgatccct cttggtttat ttaccgcaag
     6721 cagaacaggg cggggaggtt ttacttctcc ccgcccgccg tctgtatctc ctaccggctt
     6781 tccgtcgcat attactttct gtccatcttc tacacgctgc cccatctggg caacttttcc
     6841 atcaattaaa actttgccct cttggatcag ccggtctgcc tcccgcctgg aacacgctcc
     6901 catgcggctg agccatttat tcagacgttc ttccatttaa cttcctacgc tttctttaca
     6961 ggctgtggat catcagctat gaacatggca tctccgaaag agaaaaatct gtatttttcc
     7021 ctgattgcct cattatacgc tgctaaaatg ttctctcttc ctgctaatgc gcttactaac
     7081 attaccagtg tagactccgg taaatggaag tttgtgatca gcgcatccag gatcttaaac
     7141 ttatatccag ggtaaatgaa gatatcggtc cagcctgttt ttgcctgtaa aatgccgtct
     7201 tctgtggaag cagactctac tgtacggcaa ctggtggttc ctacacagat cacacggcct
     7261 cctgctgctt ttgtatcatt tacctttttt gcctctgcct cttctactac ataaaattca
     7321 gagtgcatat ggtggtctaa aacattttcc acctttacag ggcggaaagt gccaagacct
     7381 acatgtaagg taacgtgggc gatctttact cccatatctt cgatctgctt taacagttct
     7441 tttgtaaaat gaagtcctgc tgtaggcgct gctgcagaac cttcatggac tgcataaaca
     7501 gtctgataac ggttcttgtc ttttaactgg tgtgtaatgt atggaggcag aggcatctga
     7561 cctaagcggt ctaagatctc ctcaaagata ccttcataag agaactggat cagtctgttt
     7621 ccatcatcta cagtctcaat aaccgttcct gtaagaatac cgtcaccaaa gtcaataacc
     7681 gttcccggct ttgccttttt accaggtttt accagagtct cccagatatc attttcacgg
     7741 cgttttaata aaaggacctc gatcttggct ccggtgtcct tttttacacc aaataatctt
     7801 gcaggaataa ccttggtatc attaatcaca aggcagtctc ctggtcttaa atactctaca
     7861 atatccttaa agatcctgtg ctgggtcttt cctgttttct tgtccagaac taaaagtctg
     7921 gaagaagaac ggtcctccaa aggatcctgt gcgatcagtt cctgtggcag gtcaaaataa
     7981 aaatctttta catccatttt ctattacttc cttaatttat aaattaattg ttcacatgta
     8041 atctacgcaa atatgtctat gatctgtatg ctcttactgg atcgcaacag ctgcgtcaga
     8101 agactctcct gcagactccg gcgcttctgt ctgtgcttcc tggctttctt ctgctgaagt
     8161 ctcgcctgca gcgctgcttt catctgcatt gccttcacct gcatcagaag aagcatctgc
     8221 tccatcaccg atcataacct ggctgtctgc gccttcttct gaactgctta ctactgcacc
     8281 attgcttaaa tccttgtaaa taccggctaa cagactgtca gactcaacgg cctgttttaa
     8341 acgctcattg acctgtgtag ataaaaggcg cacatcctct gtctggttct ggttggcaac
     8401 gtaatctgcc tcatctccta ctacattttc gcggatgata taatcgccgt tatcattttt
     8461 cactacataa caccacataa ggcccggtgc cagcgtatct accctgcgga actttacatc
     8521 ataggttaca tatactacat aagagtcatc tgtcattccc gcctttgtat agcaggtaat
     8581 atcctgataa tcttcaatgt aaactgcttc atcttttaac tgctgttttc tttcttctaa
     8641 acggtcatct tcctctttac cgaatacttt gtaaagcagc tgaacatcct ggtctacttt
     8701 tgcctggaaa taggtactga tcagctcatt gacctgtgga taagcatcct tctgaagttc
     8761 atatgcggaa aaatcctgat gatattcatt attgtccggc tctacagatc catcatccgt
     8821 gccggtctct gtatcatttt caatggaaat atcagaagca tttgtgactt ctgcagatgt
     8881 actgcttccc tgcgccttat caccggccgg ttttctgtcc ataactaaaa tgaccacaag
     8941 taataccagt acaatggctg caggtatcgc taacattctg gcatactttg ttttatcatc
     9001 aaaagccttt cctgactttc tccttcctct gttactgtgt ctcatgttct atcattcctc
     9061 cattatttgc cactactccg ctctattcta acaaaaagca ggctaaaatg caatacagcg
     9121 aaaatcggcg gatacgtcaa aaaaactccc gtatcctaag gaataagccc ttttttaccg
     9181 aatacggaag tttttaagat tacaggtcaa ttttattaac ttaactttaa gtctccttct
     9241 ttgacccagc cgatcttctt taagaacata ttgatgatcg ggcatatcac tgcacgaagg
     9301 atttaacaaa tgggctaagg caggtctcac cgcttt
//
LOCUS       DBMDUA010000108         9199 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3511_length_9199_cov_3.420202, whole genome shotgun sequence.
ACCESSION   DBMDUA010000108 DBMDUA010000000
VERSION     DBMDUA010000108.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9199)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9199)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9199
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3511_length_9199_cov_3.420202"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            322..1254
                     /locus_tag="ACZPCL_08800"
     CDS             322..1254
                     /locus_tag="ACZPCL_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1162600.1"
                     /translation="MYLYGFDIGGTKCAVILAKVEGDQVDFLERYEMKTLGDWKKVLD
                     ELSENALMIAKKYGLTTGTEGENCVICGGISCGGPLSPDRKWILSPPNLQGWDQVPVV
                     EYLEEKLGIPVKMENDADAGALAEWKFGAGKGCQNMIFLTFGTGLGSGLILNGQLYRG
                     STGMAGEAGHIRMENEGPKGYGKVGSLEGFCSGGGISRLAGYFGTEGSAKELAERAEA
                     GDERALAVYARCGAVFGRGLAILIDLLNPEKIVAGSVYARSHHLLDKTMYEELEKEAL
                     PMNRAACEIVPAALGERIGDYAAVVAAVNSLSIL"
     gene            complement(1232..2500)
                     /locus_tag="ACZPCL_08805"
     CDS             complement(1232..2500)
                     /locus_tag="ACZPCL_08805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR1162601.1"
                     /translation="MKRLPAGIISTLLSLTMTLTPCSMSALAAQENSRDFSLFFSGPG
                     VAAFSSGDEYSEYQWALNNTGRLRRTEKVLNIKTLDHIYLHYGENGIDDIALPPLGPD
                     NFESINTDAVANIDINIEDAWKTYSETENKRTVTVAIIDTGIDTTHSDLKDSIWVNED
                     EIPGDGIDNDGNGYVDDVNGWNFVSNSNEICTGEEDSHGTHGAGTIAAAWNNGGIAGI
                     TDSTHVKLMVLKALGGSEGKGSPESVIEAIKYAEANGADICNLSFGSSNCTPEFEAAI
                     RDSKMLFVVAAGNGNQYQIGYDIDKSPVYPASLPYDNVITVGNLLFNGHLDESSNYGA
                     TSVDLAAPGTYILSTIPGDSYAYMSGTSMAAPMVTGAAALIYSARTDLSLQDIKTAIL
                     STVHKLAPLKGKTATGGMLDVSAAIKWTKN"
     gene            2911..3723
                     /locus_tag="ACZPCL_08810"
     CDS             2911..3723
                     /locus_tag="ACZPCL_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024803.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1162602.1"
                     /translation="MYQKYSIGTMAKLMGISAEAIRYYESRNIISPVRDPETGYRYYN
                     TWDFHMLLRARHYQNYGFSLEEIAELFRSHELGEIRGKMVDQEEMIQQEIIRQMNLLK
                     RIRQSQQVLQDAKDSVGKFRIEERPGIYRMNTQKNYTLFKKKEQLDLISEWTEKEPFV
                     FSCAVFYQKNIENGESEFDFGMGLREEYAQFLHVKESELVQYYPPCPCVHTCVPSRSG
                     KYLSLESLQEGFRYLKQNGLSLAGDIVTQVACMTKPEEEYFNWHIVWFPIEK"
     gene            3879..4661
                     /locus_tag="ACZPCL_08815"
     CDS             3879..4661
                     /locus_tag="ACZPCL_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1162603.1"
                     /translation="MSEQKKTVLITGGAMGQGRPHAVKYAQNGFNVVLADMLDPEDER
                     FQETIKELNELGAEVLAVKANICSTPDMENLFAKAWEKFGRLDVVIANAGVINFGNTW
                     ELTDEQVEKVINIDLIGTWRTDKYATQYMLKQGFGRIINIASTSGLYGTPKLATYCMA
                     KWGVLGLTKTLAKEVGSKGIIVNALCPTKVKTPMCETQSYVEFINELTGKNFKDYKEM
                     YAVVPFLDVEDISDMVYWLGTSRAAGKFNGRDVALDLGTLQC"
     gene            4743..6149
                     /locus_tag="ACZPCL_08820"
     CDS             4743..6149
                     /locus_tag="ACZPCL_08820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1162604.1"
                     /translation="MGNFGFKNVSKRNKIDLMEYLCYGLGDFSFCFIYGAIGSYIVFF
                     YTDVACISAATVGTVLLVSKIFDGISDMMMGYIIENVHSPLGKARPWLLWMVIPYCIG
                     CVLLFTVPNFSETGKVIYAFISYNLMATCIFTSMNVPYGVLSSVMTNKQNERALLSIS
                     RASLGALGVFVISSYAPDLVEKFGGGPAGWQKTFLLVGVVSIVLFLITFYGCKERVGS
                     GVMEQKTGKKSSLSLLQGVKILFANKYWFIMLMVNIFYTAMTSLYGMNMYFAKYVMQD
                     AGYNKTMMMCSTFASILVPLLLIPVVQKIGKRNVALYGGAGMGIAGQIVLILFAEQSM
                     GLMYLGLILRGMGVACISATKFGMIADSIEYGEYKTGTRAEGFVYSAASLGVRVGSAL
                     ASAVIGWVLGAYGYDGKLAVQSETAMKGIRIMFYYAPLAVFIAILALLLFYKLDREYD
                     GIMKVLDERHKLAEQANA"
     gene            6187..7410
                     /locus_tag="ACZPCL_08825"
     CDS             6187..7410
                     /locus_tag="ACZPCL_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate MFS transporter"
                     /protein_id="HIR1162605.1"
                     /translation="MNITKATRWRVFAGVWLQSLFTSATAYFSLFCIPLTTKFGWSDS
                     AFALAYTIYMFTYCAVGFIGGSLAEKISPRKTIYIGLCLFAGGWFLTGFASSIPQLYV
                     FYGLMAGAGGGMIYPACLPTALKWFPDRSGSISGLVQAGASCGPFIMSPIAQTLIDRV
                     GAQITCRILAIVFLIGVGIAAAMIVPCPEGWTPEGWKPSAEQAKVLKTKDYSMGEMVK
                     TPVFWVLLLMFIFANAAGTMMVSFTSPIAQRQVGQTAMTAAFCVSIMTLANMCGRIGF
                     GFIYDILKGWKSLILLMVINGASMLMLTQATTLPYFIVCIVLVGFSFGGLLVVFAPMV
                     RMIFGSKFYNRNYGLIFIGYGIGAFVGPKISAYFYDTTGQYTMGFIGSAVLAVAAIVL
                     IVIAQKMAEKMENAQ"
     gene            8932..9036
                     /locus_tag="ACZPCL_08830"
                     /pseudo
     CDS             8932..9036
                     /locus_tag="ACZPCL_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718136.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
     gene            9046..>9199
                     /locus_tag="ACZPCL_08835"
     CDS             9046..>9199
                     /locus_tag="ACZPCL_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1162606.1"
                     /translation="MNQYEILKHYFGYDTFRDGQEKLIRAILEGRDVLGIMPTGAGKS
                     LCYQIPA"
BASE COUNT         2611 a         1771 c         2338 g         2479 t
ORIGIN      
        1 tccagctgat caaagatggt gctctgatct gtacaatggc tcagaatcct gatgttatgg
       61 gttatgaagg catgtatgca gcgatcaccg ctattaatga tggaaaagta gctcctgaat
      121 atattgatac tggcgtttct atcttaaatt tagatgcagt taaataatca ggctgtttaa
      181 agaaacaaag tgaggaaaaa tatgctccgg caggtatatc agcctgccgg agctgttttc
      241 attgctgcac ataatgtact ctcgcaaagg caagtgcagg aaagactccg agagtacatc
      301 atagaagaag ggagagactg tatgtatctg tatggatttg atattggagg cacaaaatgt
      361 gcagtgatcc tggcgaaggt ggaaggagac caggtagatt ttctggagcg gtatgagatg
      421 aaaacactgg gagattggaa aaaggtcctt gatgaattat ctgaaaatgc tttgatgata
      481 gcaaagaaat atgggctgac aacagggact gagggtgaaa attgtgttat ctgtgggggg
      541 atttcctgtg gcggtccttt aagtcccgac aggaagtgga tattatcgcc gccgaatctt
      601 cagggctggg atcaggtgcc tgtagtggaa tatcttgagg agaagctggg aatccctgtg
      661 aaaatggaaa atgatgcaga tgcaggagct ttggcagaat ggaagttcgg ggccggaaaa
      721 ggctgtcaga atatgatatt tcttactttt ggcacaggtc ttggttccgg tcttatcctt
      781 aatggacagt tataccgggg aagtaccggt atggctgggg aagcaggaca catacgtatg
      841 gaaaatgagg gaccaaaagg atatggaaaa gtaggtagcc tggaaggttt ctgctctgga
      901 ggaggtatca gcaggcttgc cggatatttt ggcacagaag gaagcgcaaa ggagctggca
      961 gaacgggctg aggccgggga tgagagagct ttagcagtgt atgccagatg cggcgctgtt
     1021 tttggaagag gtctggctat ccttatagac cttttaaatc cggaaaagat cgtggcaggt
     1081 tctgtttatg ccaggagtca ccatctttta gataagacca tgtatgaaga actggaaaaa
     1141 gaagcgctcc ccatgaaccg ggcggcctgt gagatcgtgc cggcggcttt aggagagcgg
     1201 atcggggatt atgcggctgt agttgcagct gttaattctt tgtccatttt atagcggcag
     1261 atacatccag catgccgcct gttgctgttt tgccttttaa cggggccagt ttatgtacag
     1321 ttgataaaat agctgttttt atgtcctgaa ggcttaagtc agttcttgca gagtagatca
     1381 gggcagctgc gcctgttacc ataggggctg ccatggaggt tccgctcata taggcgtaag
     1441 aatcaccggg aatagtgctt aaaatatagg ttccgggggc tgccaggtct acggaagtgg
     1501 caccatagtt ggaactttca tctaaatggc cgttaaaaag cagattgcct acggtaatta
     1561 cattgtcgta gggaagactt gccgggtata caggggattt atcaatgtca taaccgatct
     1621 ggtactggtt tccatttcct gcagctacta caaaaagcat tttggagtcc cggatggctg
     1681 cttcaaattc gggagtgcag ttactggaac cgaagcttaa gttgcagata tcggcaccat
     1741 tggcttctgc atattttatg gcttcgatga cgctttcagg ggaacctttg ccttcagaac
     1801 cgcctaaggc ttttaatacc ataagcttaa catgggtgct gtctgtaatg cctgcgatgc
     1861 cgccattgtt ccaggcggcg gcaatagtgc cggcaccatg ggttccgtgg ctgtcttctt
     1921 cccctgtgca gatttcattg ctgtttgaaa caaaattcca gccattcaca tcatctacat
     1981 agccatttcc gtcattgtca atgccgtctc cggggatctc atcttcattg acccagatgg
     2041 aatcttttaa gtctgaatga gtcgtatcga taccggtatc gatgatagct acggtaacgg
     2101 tacgtttgtt ttctgtttcg ctgtatgttt tccaggcatc ttcaatgttg atatcaatat
     2161 ttgccactgc atcggtgtta atggactcga aattgtccgg tcctaaagga ggaagggcaa
     2221 tatcatctat gccattttcg ccataatgga gataaatatg gtccaaggtt ttgatgttca
     2281 gcactttttc cgtgcggcga agccgtccgg tgttgttaag agcccactga tattcgctgt
     2341 attcatcgcc ggaagagaag gcagctacgc caggaccgct gaagaaaagg gaaaaatccc
     2401 ggctgttttc ctgggctgcc agtgcggaca tggaacaggg ggtcagcgtc attgtaaggg
     2461 aaagcagggt ggaaatgatg cctgcaggta gtcgtttcat gaaggggcac tcctttcgtg
     2521 gatagtcgaa ggacactcac gaacccgcgc tgacgcgctc tgctgtttga tggtgcagac
     2581 ggttcgtaag gatagtcact cacgaacccg cgctgacgcg ctctactgcc tgctgacgca
     2641 ggcggttcgt tcgttggtgg caccgaaaaa caaatgtctg ctgcgcagcc gcttgttttt
     2701 ctttcgtgcc taccacatta taccatgcca gctgtgatga aagtatgaca tttttctgaa
     2761 gaaatatggt atacttgtta tattcttgtt aaaaatcttt caaggggctt gagagtttga
     2821 aatgtaaaca gttgtgaaaa ttatgtctaa caggtgaaag cagggaaaca tgttttctta
     2881 gagaggtcat tcaattgagg gggaaataag atgtatcaga agtacagtat tggaaccatg
     2941 gcaaaactga tgggtatctc ggcggaggca atccggtatt atgagagcag gaacattatc
     3001 agtccggtcc gtgatccgga aacaggctac agatactaca atacctggga ttttcatatg
     3061 cttttaaggg ccagacacta ccagaactat ggtttttcac tggaagagat agcagaactg
     3121 ttcagaagcc atgaattagg ggaaatacgg gggaaaatgg tggatcagga agagatgatc
     3181 cagcaggaga tcatccgcca gatgaacctt ttaaagcgga tcagacagag ccaacaggtg
     3241 cttcaggatg caaaagacag cgttggaaag ttccgtattg aggaaagacc gggcatttac
     3301 cgcatgaata cccagaaaaa ctatacttta tttaagaaga aagagcagct ggatctgatc
     3361 tcagaatgga cagaaaaaga accttttgtc ttttcatgcg cagtatttta tcagaaaaat
     3421 attgaaaatg gagagtcaga gtttgatttc ggaatgggac ttcgggaaga gtatgcacaa
     3481 ttcttgcatg taaaagagtc agaactggtg cagtattatc cgccttgtcc ctgcgtccat
     3541 acctgtgttc cctcacgttc cggaaaatat ctgtcactgg agagtttaca ggaaggtttc
     3601 cggtatttga agcagaatgg attaagcctt gcaggggata ttgtcaccca ggtagcctgc
     3661 atgaccaagc cggaagagga gtattttaac tggcatattg tgtggtttcc tattgaaaaa
     3721 tgattgtgca gagtgcaata aaccactctg cattttttgt gcaaaaaatc aacttgacct
     3781 taaaaccact ttagttgtta tagtagtggc agatgagcaa gttaaaaaaa taacaaacac
     3841 atttaaacca cttaaaaaag tgaaagggga aaaaggtcat gagtgaacag aagaaaacag
     3901 tattgatcac cggaggagct atgggacagg gacgtcccca tgcagttaag tatgcacaga
     3961 acggatttaa cgtagttctg gcagatatgc tggatccgga agatgaaaga ttccaggaaa
     4021 ccattaagga attaaatgaa ttaggcgctg aagttttagc tgttaaggca aacatctgca
     4081 gcacacctga tatggaaaac ttatttgcaa aggcatggga gaagttcgga cgcttagatg
     4141 tagttatcgc aaacgcaggc gttatcaact tcggaaatac ctgggagctg acagatgagc
     4201 aggtagagaa ggttatcaac atcgacctga tcggtacctg gagaactgac aagtacgcaa
     4261 cacagtacat gttaaagcag ggctttggaa gaattatcaa tatcgcttct accagtggtc
     4321 tttacggaac accaaagtta gctacctact gcatggctaa atggggcgtt ttaggtctga
     4381 ccaagactct ggcaaaagaa gttggaagca agggtatcat tgttaatgct ctttgcccaa
     4441 ccaaggttaa gactccaatg tgcgaaaccc agagctatgt agagttcatc aatgaactga
     4501 ccggaaagaa ctttaaggat tacaaggaaa tgtatgcagt agttcctttc ctggatgttg
     4561 aggatatttc tgatatggtt tactggcttg gaaccagccg tgcagctggc aagttcaacg
     4621 gcagagacgt tgccctggat ctgggaactc ttcagtgcta atcactgaat aaaaatggac
     4681 atgctgccag acagtggcat gtccatgaat aaaagcaaac agaagttggg gacaaaagaa
     4741 aaatgggtaa ttttggtttt aaaaatgtat caaagagaaa caagattgat ctgatggagt
     4801 acctttgcta tggactggga gatttctcct tttgcttcat ttacggagcc atcggttctt
     4861 acattgtttt cttttataca gatgtggcct gcatcagtgc agctacagta ggaacggtac
     4921 ttttagtcag caagatcttc gatggtattt ctgatatgat gatgggatat atcattgaaa
     4981 atgtccattc accgctggga aaagcaaggc cgtggctatt atggatggta attccgtact
     5041 gcatcggctg tgtgctgttg tttacagtac cgaatttcag tgaaacagga aaggttatat
     5101 atgcatttat ttcctataac ctgatggcaa cctgtatttt cacttccatg aacgtgccat
     5161 acggtgtttt aagcagcgtt atgacaaata agcagaacga aagagcactt ctttctatca
     5221 gccgtgcaag tttaggagct ttaggcgtat tcgtgatcag ctcctatgca cctgatctgg
     5281 tagaaaaatt tggcggcgga cctgccggct ggcagaagac attcctgtta gtaggtgtcg
     5341 tttctatcgt actgttttta attacatttt atggatgtaa agagcgtgtt ggttccggtg
     5401 ttatggaaca gaaaacagga aagaaatctt ccttatcttt attacaggga gtgaagatcc
     5461 tgtttgcaaa caaatactgg ttcatcatgc tgatggtcaa cattttctat actgcaatga
     5521 ccagtcttta cggcatgaac atgtattttg caaaatatgt tatgcaggac gcaggctaca
     5581 ataagaccat gatgatgtgt tccacattcg catccatcct ggttcctctg cttctgatcc
     5641 cggttgtaca gaaaatcgga aaacgtaatg tggctctcta tggaggcgca ggaatgggta
     5701 ttgcaggaca gatcgtcctc attctgtttg cagagcagtc catgggactt atgtatctgg
     5761 gactgatcct tcgtggaatg ggtgttgcat gtatttccgc taccaagttt ggtatgatcg
     5821 cagacagtat tgagtacgga gagtacaaaa caggtacccg tgcagaaggt ttcgtatatt
     5881 ccgctgcatc cttaggtgtc cgtgttggtt ccgctcttgc atctgcagtc atcggctggg
     5941 tactgggagc ttatggatat gacggcaagc tggcagtgca gagtgaaact gcaatgaaag
     6001 gcatccgtat catgttctat tatgcgccgt tagctgtatt tattgctatt ctggcactgc
     6061 tgctgtttta taaactggac cgtgagtatg acggcatcat gaaagtctta gatgagcgcc
     6121 acaaacttgc agaacaggca aatgcataaa gttttacaag tcacattttt aaggagagtt
     6181 tagagtatga atatcacaaa agcaactcgc tggagagtat ttgccggtgt atggctgcag
     6241 agcttattta cctctgctac tgcttacttc agtctgtttt gtatcccgtt aacaaccaag
     6301 tttggatgga gtgattccgc attcgcactg gcatatacta tttatatgtt tacatactgt
     6361 gcagttggct tcatcggcgg ttccttagct gagaagatca gtccaagaaa aaccatttac
     6421 attggtcttt gccttttcgc aggcggctgg ttcttaaccg gttttgcatc cagcatccca
     6481 cagctgtatg tattttacgg attaatggct ggtgccggcg gcggtatgat ctacccggca
     6541 tgccttccta ctgcattaaa gtggttccct gaccgttccg gttctatttc cggtctggtt
     6601 caggccggcg cttcctgcgg tcctttcatt atgagtccta ttgcccagac tctcattgac
     6661 cgtgtaggcg cacagataac ctgccgcatc ctggcgatcg tattcctgat cggtgttggt
     6721 attgcagcag caatgatcgt tccttgtccg gaaggctgga ctccagaagg ctggaagcca
     6781 tcagctgagc aggctaaggt attaaaaaca aaagattaca gcatgggcga aatggttaag
     6841 acccctgtat tctgggtact gttattaatg ttcatcttcg caaatgcagc aggaaccatg
     6901 atggtttcct tcacaagccc gatcgcacag cgtcaggttg gtcagactgc aatgaccgca
     6961 gcattctgcg tatccattat gacactggct aacatgtgcg gacgtattgg ttttggtttc
     7021 atctacgaca ttttaaaggg ctggaagagc ctgatcctgt taatggtcat caatggagct
     7081 tccatgttaa tgctgacaca ggcaactaca cttccatact ttatcgtatg catcgtttta
     7141 gtaggcttct ccttcggcgg actgttagta gtatttgcgc caatggtacg tatgatcttc
     7201 ggttccaagt tctacaaccg taactacgga ctgatcttca tcggctatgg tatcggtgct
     7261 ttcgtaggac ctaagattag tgcttacttc tacgacacca ccggccagta caccatggga
     7321 ttcatcggat ctgcagtatt agcagttgca gccatcgtac tgatcgtgat cgcacagaag
     7381 atggcagaaa agatggaaaa tgcgcagtaa gaaatgaagt tacagttcag taacaattaa
     7441 atagtaaaat gtttataaaa accctggaaa ctggtgcagt tgtgtgcctg gtttccaggg
     7501 tttttaaaat aaaatgcttg aagcatatgc ccttctgcct tataataaca gggaaagacc
     7561 cggaaggtta catgatttct gggctatttt tttgaaatga agaaatgagg ataagacaga
     7621 atggaaataa aagcacagat caccaaaaaa ctgcagaaat acaaagcgga tgagatcaaa
     7681 actctgtatc agctgatgct cagagtatta tccggcaatg atgattttcg catggtacaa
     7741 aatgcagata cagatgatct gcgtcctgta atcgaagggg agacaggaga aaagatcgac
     7801 tttgcaatgg agtccattat ttcaggagag tcactgaaac tgtggcagga catccaggca
     7861 gaggaagcag aaaaagtaaa attaaagaag gcagacagaa ttgaaagaat tgcttctgtt
     7921 atgagcgatg ctgctttatt tgaaggtttc tgcctggctt tttatggaaa agatgagatg
     7981 ctggcagttc tctgcgatga gtatggctgt ctggaagctt atgaagcctt agtacaggat
     8041 actgtataca gaaaaagaag agcttatatg cgtatgcttt ctgactatgt gctggcagcc
     8101 tcccacctgt atggtgtagt agctcttcat gattttgaac tgctgctgcg ccattacgaa
     8161 aagaacctgg atgatttcga tggctatgct agagaagaag gaagctataa aaacaccatc
     8221 cttttccagc caagatactt agggctttgt accttacagc agctgatcgg aaatacagta
     8281 ccagaagtac tgactaccat ggacggcctg tttttacatc cttcttttac agaagatttc
     8341 cagaaagaac agaaggaaat ggtaaaggca tttgcgaaga atggtggaca gaatgtggga
     8401 gaaaaggatt tcgaccagtt ctttgcagct gtaggggaaa aaacttccta cagaaagctg
     8461 ctgcgtgaca ccatggacaa gccaatgtat cttccaacca aagaagaatt tttaaaatac
     8521 gtaaacgaag attaccatgt gatctcgact gcagaaaaga atttaagaaa ctatctgaag
     8581 ggaaaatatg gaaaagagtt agaaacagca gcacaggcag caggaactac cgcggaggta
     8641 tacttagaag cctttattgc aaagctgcgt gatttaggca cagatgtagg aaaaggcgga
     8701 gcagagccgg atcctcaggc acagatccag tttgttttaa ccacattaag acagatcggc
     8761 gctgcttttg agaatattgc agacgcaaaa gaaccactgc gctatgcaat ggaactgtcc
     8821 aatgcagagc atttatggtg caaccgtgga tttagcgcag acggactggc agtgcgtatg
     8881 gctgcagaaa agaaaaatac agacattgca gccggtcttt taaatcaggg caaggcaaat
     8941 gggcaggtag tcagaaagac tgcaaagatc tatccaaatg atccatgccc atgcggaagc
     9001 ggcaaaaagt ataagaagtg ctgcggaaag aattaggcat aaaatatgaa tcaatacgag
     9061 atcttgaagc attattttgg atatgatacc ttcagggatg gacaggagaa actgatccgt
     9121 gctatcttag aaggaagaga tgtccttggc attatgccaa caggggcagg aaagtccctt
     9181 tgctatcaga taccggcac
//
LOCUS       DBMDUA010000109         9050 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3566_length_9050_cov_4.528551, whole genome shotgun sequence.
ACCESSION   DBMDUA010000109 DBMDUA010000000
VERSION     DBMDUA010000109.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9050)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9050
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3566_length_9050_cov_4.528551"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(4..495)
                     /locus_tag="ACZPCL_08840"
     CDS             complement(4..495)
                     /locus_tag="ACZPCL_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1162607.1"
                     /translation="MDEKKFAIKETVSAEEVKMLRKKLGLTQRDFAGLLGCSKPTVER
                     LEREGTVTKGPMALLMKLLDRDAEYIQTLMIPPRELPVRIWYMYKDTPCTLIDVDEMQ
                     KIVHIRNYVDNIQFRAFGIKENPTIEDYNEFLESRCFPRTRDKMKLVLRDLGKHLDSK
                     NSL"
     gene            780..1355
                     /locus_tag="ACZPCL_08845"
     CDS             780..1355
                     /locus_tag="ACZPCL_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR1162608.1"
                     /translation="MKASVLYKWLTYSAIAISILLCLFMLQAGLKGNFRSVDTFQAYI
                     RGFGLFAPFVFVFIQAFQVVVPVLPGFIGCVAGALLFGSVCGFVYNYVGICAGSIIAF
                     FLAKHFGIPLVQAMFPSKKYNVWTERIKRARSYDVLFALSILLPLAPDDYLCYFSGLI
                     NMSVKKFVLIILLAKPWCILFYSFGTGMLIQ"
     gene            1507..2145
                     /locus_tag="ACZPCL_08850"
     CDS             1507..2145
                     /locus_tag="ACZPCL_08850"
                     /inference="COORDINATES: protein motif:HMM:NF016214.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR1162609.1"
                     /translation="MLGRTHFFIGISAALAVMQPSSMPTLVAGAGAAAIGGMISDIDS
                     GTSQAHREADKIMTAAIAVTAIVILADYKFHVGIYERLMRNSSIARLLTGAMAFIIIC
                     TYGSSQPHRSFMHSFLSLFMLTACIDVIYPDAAPYFAVAYASHLALDLLNYRRLRLIW
                     PMKRGFSLGFCSSQGLINHLLMSAGMVCVVLELVTSIPARRLAERIFSFLPF"
     gene            2301..3161
                     /locus_tag="ACZPCL_08855"
     CDS             2301..3161
                     /locus_tag="ACZPCL_08855"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR1162610.1"
                     /translation="MKPVKEGKVREIYDNGDSLIMVATDRISCFDVILHNTVTKKGTV
                     LTQMSKFWFDYTKDILPNHMISTDVKDMPEFFQTPEYEGKSMMCRKLNMLPLECIVRG
                     YITGSGWKSYKENGTVCGIKLPEGLKESDKLPEPIFTPSTKAEIGDHDENISYEQSID
                     HLEKHFPGKGKEYATKLRDYTIALYKKCADYALSKGIIIADTKFEFGLDEEGNIVLGD
                     ETLTPDSSRFWPAEGYEPGHSQPSFDKQFARDWLTSHPGNDWTLPEEIVQKTIDKYLQ
                     SYKMLTGKEL"
     gene            complement(3282..4367)
                     /locus_tag="ACZPCL_08860"
     CDS             complement(3282..4367)
                     /locus_tag="ACZPCL_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162611.1"
                     /translation="MFWNMDVGEQEQDLMEISHFLLSVFEFAKNQPGNVFQKEDWLDF
                     SPENGTWFYRLYERDTDNGKNMRRAVDRLFRMDQEKREEIYEAVKHDMEFMDKKAWEK
                     GKFNLESDQLPEAAQKILRDFFGYFYDVVLCTTHFRLEGMSRPFYSRKELAKDYFKGK
                     NEKIRRICPVCLQPVTNGETDEDVEHYFPKSKYPCLSLHPYNLYFCCTTCNTRFKGTK
                     SPLKGKTRDIGRIFLPYLDTVKDAVQLKFENPGDKDSEVVKMLPKGDTVPETKEKIRE
                     FDRLFALEERWSGQLECYYMSLYQRYQEKYGDGEMSLEKLKEYLEEDIKRNQIIQNWQ
                     PGRYLEGEYMKWIANKQLKAFYEELKK"
     gene            complement(4399..6042)
                     /locus_tag="ACZPCL_08865"
     CDS             complement(4399..6042)
                     /locus_tag="ACZPCL_08865"
                     /inference="COORDINATES: protein motif:HMM:NF024573.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1162612.1"
                     /translation="MKLEYLYIDGYKGLKRLKLQFKEQTGVVPVDFLIGCNGSGKSSV
                     LEAVGLIFTRIMQNELPGFIFELKYRMPDGTGIYVKPQKKGFCDASGRRRKLYVELEK
                     NGKTQQLYSIPDEYLPDRIISYCSGANSSMEDILIRSPRASLASDLYDLSLAMDVENG
                     EGPVSGKEEHPGESSVARGEAAEEILSFYERLDVNPRVLFLDAVTSKFILPALFAVMP
                     FDMQDDRISEKALRYATLRKKLLKRLNIRLIPAAFSLRIDDTRLEEAADRPQMSILRR
                     LLSQDVKGKALSNCVIHRTSTDRLEEDGSLAGETAAVFLFESWEAEGEKVFYHPMLQR
                     YFDGNPFALISTLLTAWREGVIREIHFSYRNGDERGLYEMEDLSDGELMWLARIGLIL
                     MAQNHCGENTLFLYDEPDVHFNDDWSRDFVRFVYAMCSMDGTSGNEFLVATHSDLILT
                     DAMRSQIHLFENPTGSRTEVKELEISTFAAGRDSISKQVFKASSIGGFASDTIKELMA
                     ETDPEQLAQAISKIGPGYQRFRLQEQLYALLEKNDRIKG"
     gene            complement(6051..7337)
                     /locus_tag="ACZPCL_08870"
     CDS             complement(6051..7337)
                     /locus_tag="ACZPCL_08870"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIR1162613.1"
                     /translation="MENWKQKPLSQVVSRWIRGTTLNRSRADYYTKTPGPESLPWARV
                     GDMKEGLLCETENYLTKEGVDQIPWLIVPEGAVLLSVSGTIGKSAIAGCDLVVNQAIQ
                     AMIFDEGQILPEYACFYLEFYRPWLIERANAVTVPNLTKEQLSGIPVVFPCLEEQQVI
                     VDQLKRARRLMQRSRRSEDTLNRILENAFGKIARSALKEGKISRDEEFLSPVLRPIWV
                     SLKTRVLPAEHETDMFVPVLSQTEQVSFIKIVERTKEIRKRLHKIQQLEIRYFKSMLS
                     LAFTAGLTEGFRKQEDLSDPEPALFRESYGIGNVRNVSQPTEGITDWQSRIPQELQSL
                     FTMLSDFQMEILRIYAQSQEAIPVHTVFKQIHKKGYSVQDALASARLLEALGFLEKTV
                     PQKLYMGEKEVRDSAGHPITIQKYQIPEYGADIREV"
     gene            complement(7340..8728)
                     /locus_tag="ACZPCL_08875"
     CDS             complement(7340..8728)
                     /locus_tag="ACZPCL_08875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdM family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HIR1162614.1"
                     /translation="MLTSDQNRKINEIVEEFKEAADFYRMEYSEDLMGMFRDFGCMLL
                     LKCKVDTEGKADLERLLEGRHKLKGTVKRQLMTVSEELVQLWAPGEQDGSGDTGEYEG
                     SQNVREMFQTALEEIFHFCLYSKGGKNRYLLPEETAKPLLRLLTFPEGMMAGNEGLVV
                     LDTQCGAGSALMAASRYLKNLRLMGYEKDEEMWAAAVIMSQLSGLEMELHLEDGARAD
                     LYESCDLVISNPVYGTDTIKDEELIDQLPGELKTVRGRYHMELIRSMQALNFDGKAML
                     IVPNSFLFANRTESMKVRKWLLQSYSLEAIISLPEDTFMYSGVRSGVMVFSKPFMSGG
                     WEGHTQRVLYYNLEKQEDKEKQQKEYERLEEVWSQRDSYYGKWERSRREKTVENRNGI
                     KVPANWQYNSFWFADVDQIEAGKWNLLPENYRPEEQINLEIEDPALLLQEMLKEQEEI
                     TKELNELLKEVM"
BASE COUNT         2285 a         2298 c         1757 g         2710 t
ORIGIN      
        1 ggattataat gaatttttgg aatcaagatg ctttccaaga tctcttaaca ccagtttcat
       61 cttatctctc gttctaggga agcatcttga ttccaaaaat tcattataat cctcaatggt
      121 tggattttcc ttgatcccaa atgccctaaa ctggatatta tctacataat tacgaatgtg
      181 aacaattttt tgcatttcat ccacatcaat cagggtacat ggcgtatcct tatacatgta
      241 ccatatacga accggcagtt ctctgggtgg gatcataagt gtctgtatat attccgcatc
      301 ccggtccaat aacttcatca aaagcgccat aggacctttc gtaacagtcc cttcacgctc
      361 cagacgttcc acagtcggct tggagcaacc caacagtccc gcaaaatctc tctgggtcaa
      421 gcctaatttt ttgcgcagca tttttacctc ttctgcgctc actgtttctt ttattgcaaa
      481 ctttttttca tccatattcg cccttctcca cacctttatc tctattttta ttatagtatt
      541 atttttcctt gtttcaataa aaataatcat tttgatgatt ttttattggt attttgtcat
      601 caatatgatt acttttaatt ttgtaatacg ggattatacc atttttaagc ttatgttcaa
      661 acttccttca caattattct atattgtgat ttaatagcca ctgaacgaac cgcctgcaaa
      721 agcaggcagt ggaacgcgta agcacggggt tcgtgggtaa tacaaaggag gtcttaacag
      781 tgaaagcatc tgttttatat aaatggctga cttacagcgc tattgccatc agcatccttt
      841 tgtgtctgtt tatgctccag gcagggctta aaggcaattt ccggtctgtt gatacatttc
      901 aggcttacat acgtgggttt gggctgtttg cgccttttgt gtttgtgttt atacaggcgt
      961 ttcaggtagt tgtgccggtg ctgccgggat ttatcggctg cgtagccggt gctttgcttt
     1021 ttggcagtgt atgcggattt gtttataatt atgtggggat ctgcgccggc tcgatcatcg
     1081 catttttcct tgcaaaacat tttggtatcc ccctggtgca ggccatgttt ccatccaaaa
     1141 aatataatgt atggacagag cggatcaaga gagccagaag ctatgatgtg cttttcgctc
     1201 tttccattct tttgccattg gcaccggatg actacttatg ctacttttcc ggccttatta
     1261 atatgtcagt gaagaaattt gtccttatca tccttctggc aaaaccatgg tgtatcctgt
     1321 tttacagttt cggcacagga atgctcatac aataaatatt gattatgata acaacacatt
     1381 acttacataa tgattcttaa gagaaatgtc atttgatttt caggcataga taggatacaa
     1441 tcgaagatat cccaaaaatt ggggataatt gtaaataaaa cacttagata aggatcctat
     1501 tttaacatgc ttggaagaac acattttttc attggtatat ctgctgcttt ggcagtgatg
     1561 cagccctcct ccatgcctac tttagtagct ggtgcggggg ctgctgctat tggcggcatg
     1621 atcagcgata tcgactccgg aacctctcag gcccacagag aggctgacaa gatcatgaca
     1681 gcagccattg ctgttaccgc catcgtcatt ttggcagatt ataaatttca tgtgggcatt
     1741 tatgaacgcc tgatgcgaaa cagcagtatt gccagacttc tcaccggcgc catggcattt
     1801 atcatcatct gcacctacgg ctcgtcgcag ccccaccgct cctttatgca ttccttttta
     1861 tccctgttca tgctcacggc atgcatagat gtgatctacc cggatgccgc cccttatttt
     1921 gccgttgcct acgcttccca tctggcactg gatctgttaa attacaggcg tctgcgactg
     1981 atctggccta tgaagcgtgg cttttccctg gggttctgtt cttcccaagg gctcatcaac
     2041 cacctgctca tgtccgccgg aatggtctgt gtcgtcctgg aactggtcac ttctatcccc
     2101 gcaagacggc tggcagagcg gattttttct tttttacctt tttgaataat gatttttaat
     2161 tagtattgct tatatatcat acgtcatttt tcacaaaaat taattatact attgattttt
     2221 gaaaattttt acgtatattc tataggtaga taacaaaaaa atcccattta attaaaattc
     2281 aaacattgtg aggtagaaag atgaagccag ttaaagaagg aaaagtacgt gagatctatg
     2341 acaacggcga tagcctgatc atggtcgcta ccgaccgcat cagctgcttt gatgtgattc
     2401 tccataatac tgttaccaag aaaggtacgg ttctgaccca gatgtctaaa ttctggtttg
     2461 actacacaaa ggacattctt ccaaaccaca tgatctctac tgacgtaaaa gatatgcctg
     2521 aatttttcca gactcctgaa tacgaaggca agagcatgat gtgccgcaag ctgaacatgc
     2581 ttcctctgga gtgcatcgtc cgcggttata tcacaggaag cggctggaag agctacaagg
     2641 aaaacggtac tgtatgcggc atcaagcttc ctgaaggctt aaaggaatct gataaactgc
     2701 ctgaaccgat cttcacacct tctaccaagg ctgagatcgg tgatcacgat gaaaatatct
     2761 cctacgagca gagcattgac cacctggaga aacacttccc gggaaaggga aaagaatacg
     2821 ctaccaaact gcgcgactac accatcgctc tttacaagaa atgtgctgac tacgctttaa
     2881 gtaagggcat catcattgct gataccaagt ttgagtttgg tctggatgaa gaaggaaata
     2941 tcgttttagg cgatgaaacg cttactcctg acagctcccg tttctggccg gcagaaggct
     3001 acgaaccagg tcattcccag ccatcctttg acaagcagtt tgcccgcgac tggctgactt
     3061 cccatccggg aaatgactgg acactgccgg aagagatcgt tcagaagacc attgataaat
     3121 atctgcagag ctacaagatg ctgactggca aagaattata aaaacaaaag cccccataac
     3181 agaacgcctg gttttcctat tctccaaagt ttcctccttg gaactattac aggaacgcca
     3241 ggcgttcttt ttattggtta tcatgatgat caaaatagat cctatttttt cagttcctca
     3301 taaaaagctt taagctgttt atttgcgatc catttcatat actccccttc caggtatctt
     3361 cccggctgcc agttctggat gatctggttc ctctttatat cttcttccag gtattctttc
     3421 agtttctcca ggctcatttc cccatcccca tatttttcct gataccgctg ataaagggac
     3481 atataataac attccagctg tccgctccag cgctcctcca gggcaaataa gcggtcaaat
     3541 tcccggatct tctctttcgt ctccggcaca gtatctccct ttggaagcat ttttaccact
     3601 tcgctgtcct tatccccggg attttcaaat ttcagctgca cggcatcttt tactgtatcc
     3661 aaatacggca gaaagatccg tccaatatcc cttgtttttc cctttaacgg gcttttcgta
     3721 cccttaaaac gggtattgca ggtggtacag caaaaataca gattataggg atggagactt
     3781 aagcagggat acttggattt tggaaaataa tgttccacat cctcgtctgt ttccccattc
     3841 gttaccggct gtaagcacac aggacagatc ctgcggatct tttcattctt tcccttaaaa
     3901 tagtcctttg ccagctcttt cctgctgtaa aaaggccgtg acatcccttc taaacgaaaa
     3961 tgggtggtac acaggaccac atcataaaaa tacccgaaaa aatcccggag tatcttctga
     4021 gccgcttcag gaagctggtc actttccagg ttgaattttc ccttttccca ggcctttttg
     4081 tccatgaatt ccatatcatg tttcactgct tcgtaaattt cttcccgctt ttcctggtcc
     4141 atgcgaaaga gccggtccac agcacggcgc atattctttc cattatctgt atcacgctca
     4201 tacaaccggt aaaaccaggt gccgttctcc ggtgaaaagt ccagccagtc ctctttttga
     4261 aacacatttc ccggctggtt tttcgcaaac tcaaaaacgg ataaaagaaa atggctgatc
     4321 tccatcagat cctgttcctg ttctcctacg tccatattcc aaaacatctt ctgtcacctc
     4381 ttttcgttcc aaaagtacct aaccttttat cctgtcattt ttctctaaca aggcatacag
     4441 ctgttcctgc aggcgaaatc tctggtaacc gggaccgatc tttgaaatag cctgggccag
     4501 ctgttccgga tccgtttctg ccattagttc tttaatcgtg tccgatgcga aaccaccaat
     4561 agaagaagcc ttaaacacct gctttgaaat gctgtcgcgg cctgctgcaa aggtggagat
     4621 ctccagctct tttacttctg tcctggaacc tgtaggattt tcaaacaaat ggatctgcga
     4681 ccgcatggca tctgttaaga tcaggtctga atgtgtggct accagaaact catttccgct
     4741 ggtcccatcc atactgcaca tggcatatac aaagcggaca aaatcccggc tccagtcatc
     4801 attaaaatgc acatccggct catcataaag gaacaatgta ttttccccac agtgattttg
     4861 tgccataagg atcaggccga tccgggccag ccacattaac tcaccgtcgc ttaagtcctc
     4921 catttcatat aagccacgtt catccccgtt acggtaggaa aaatggatct cccggatcac
     4981 accttccctc caggcagtaa ggagggttga gataagagca aatgggtttc cgtcaaagta
     5041 gcgctgaagc atgggatgat aaaatacttt ctccccttct gcttcccagg actcaaacag
     5101 gaatacagct gccgtttcac cggcaaggct tccgtcctct tccagccggt ctgtagaagt
     5161 ccggtggatc acacagttgc ttaaggcttt tccctttaca tcctggctta aaagccgccg
     5221 caaaatactc atctggggcc ggtctgctgc ctcttccagc ctggtatcat caatacgaag
     5281 ggaaaatgcc gcagggatca gacggatgtt taaccgtttc agaagttttt tccggagtgt
     5341 ggcatatcgc agagcctttt cactgatacg gtcatcctgc atatcaaaag gcatcactgc
     5401 aaacaatgca ggtaagataa atttagatgt aaccgcatcc agaaacagca ctcgcggatt
     5461 tacatctaac cgctcataaa aggataatat ctcttctgcc gcctcccctc ttgcaacact
     5521 gctttctccc gggtgttcct cttttccaga taccggacct tctccatttt caacatccat
     5581 agccagagac agatcataca gatcactggc aagagaagcc cttggggaac ggatcaggat
     5641 atcttccata gagctgttgg ctccggagca atacgaaatg atccggtccg gcagatactc
     5701 atccggtatg gaatatagct gctgggtttt cccatttttt tccagttcta catataattt
     5761 tctccgccgc ccggaagcat cgcaaaagcc ctttttctgg ggctttacat aaattcctgt
     5821 cccatccggc atccggtatt ttaactcaaa gataaatccc ggcagttcat tttgcatgat
     5881 acgggtaaaa ataagcccca cagcctccaa cacactggac tttccggagc cattacagcc
     5941 gatgaggaaa tccacaggca caacccctgt ctgttcctta aactgcagct tcaggcgttt
     6001 caatcctttg taaccatcta tgtataagta ttccagtttc atgttttatc tcagacctcc
     6061 ctgatatcag caccgtattc cgggatctga tatttctgta tggtaatggg atgacctgca
     6121 ctgtcccgta cctctttttc tcccatataa agtttctggg gaaccgtctt ttccagaaag
     6181 cccaaagctt ccaacagcct tgcagaagca agggcatcct gtactgagta tccctttttg
     6241 tggatctgct taaacaccgt atgtaccggt attgcctcct gtgactgggc atagatacgc
     6301 aggatctcca tctggaaatc agacagcatg gtaaaaagag actgcaattc ctgtggaata
     6361 cggctctgcc agtctgtaat gccttctgtt ggctggctga catttcgcac atttccgatt
     6421 ccgtagctct ccctgaataa agcaggctcc gggtctgaaa ggtcttcctg ttttctaaag
     6481 ccttctgtta aaccggctgt gaaagccagt gacagcatgg atttaaaata gcgtatctcc
     6541 agttgctgta ttttatgtaa ccgttttctt atttcttttg ttctttctac tatttttata
     6601 aaagagacct gctctgtttg tgaaaggacc ggtacaaaca tatctgtttc gtgttctgca
     6661 ggcagtaccc gtgtttttaa agaaacccat ataggccgga gcacaggaga aagaaattcc
     6721 tcgtcccggc ttatttttcc ttcttttaaa gcagaccggg ctatttttcc aaaagcattt
     6781 tccaggatcc tgttcaacgt atcttccgat ctcctgcttc tttgcatcag tcttctggcc
     6841 cgttttaatt gatccacaat gacctgctgc tcctctaagc aagggaatac cacaggaata
     6901 cccgacagct gttccttcgt cagatttgga acagtcaccg cattggcccg ctctataagc
     6961 catgggcggt aaaattccag ataaaagcag gcatattccg gcaggatctg tccttcatca
     7021 aagatcatgg cctggatcgc ctggttcact accagatcgc agcctgcaat agcacttttt
     7081 ccaatagttc ctgatacaga aagtaacaca gcgccctctg gcacgatgag ccacgggatc
     7141 tggtctactc cttctttggt aagataattt tctgtttcac aaagaagtcc ttctttcata
     7201 tctcccactc ttgcccacgg aaggctttcc ggtccaggag ttttcgtata ataatcagcc
     7261 cttgaacggt ttaaggtggt tcctctgatc catctggata ccacctggct cagaggtttc
     7321 tgcttccagt tttccacttt tacattacct ccttaagaag ttcatttaac tcctttgtaa
     7381 tctcttcctg ttccttaagc atctcctgca aaagaagagc cgggtcctct atttccagat
     7441 tgatctgctc ttccggtctg taattttccg gcagaaggtt ccattttccc gcctcgatct
     7501 gatccacatc tgcaaaccag aaactgttgt actgccagtt tgcaggaacc tttataccgt
     7561 tgcggttttc caccgttttt tccctgcggg agcgctccca ttttccataa taactgtctc
     7621 tctggctcca tacctcttcc agacgttcat attctttctg ctgcttttcc ttatcttcct
     7681 gcttttccag attataatac agtactctct gggtatgtcc ttcccaaccg ccggacataa
     7741 aaggcttact gaataccatg acaccggagc ggacaccgct gtacatgaaa gtatcctccg
     7801 gcagtgaaat gattgcttcc agggaatagg actgaagaag ccatttgcgt accttcatac
     7861 tttctgtgcg gtttgcaaat aaaaagctgt tgggaacgat cagcattgct tttccatcga
     7921 aatttaacgc ctgcatgctg cggatcagct ccatgtgata acgtcccctg actgttttca
     7981 attctcccgg aagctgatcg atcagttctt cgtcttttat ggtatcggtt ccataaacag
     8041 gattgctgat caccagatca caggactcat acagatccgc ccgtgccccg tcttctaaat
     8101 gcagttccat ctcaagaccg gaaagctgtg acatgatcac ggctgccgcc cacatttctt
     8161 catccttctc gtagcccata agccggagat tttttaagta ccgggaagct gccattaaag
     8221 cactccctgc accgcactgg gtatctaaaa ccaccaggcc ttcatttccc gccatcatcc
     8281 cttccggaaa tgtgagcaga cgaagaagcg gctttgctgt ttcttccgga aggagatacc
     8341 tgtttttacc gcctttgctg taaaggcaga aatgaaagat ctcttcaaga gccgtctgaa
     8401 acatttcccg aacattttgg gaaccttcat attccccggt atccccagat ccatcctgtt
     8461 cgcccggtgc ccacagctgc accagttcct ctgatacagt cataagctga cgttttaccg
     8521 ttccttttag cttatgacgg ccttccaaaa ggcgctccag atccgccttt ccttctgtat
     8581 ccaccttaca cttcaaaagc agcatacacc caaaatcccg aaacatcccc ataaggtctt
     8641 cggaatactc catacggtaa aaatcagccg cttccttaaa ttcctcaacg atttcattta
     8701 tctttctatt ctggtctgat gtcagcatag ttttatcgct cctttatccg aagtgaattt
     8761 aacaactcac ccaagtccac ttacagccct gcgtcaacgc gctctactgt tctgcgctca
     8821 ttatatccca cttcgcccta acttgaaagt aaaaagaatg tgccccaaca taaaaaagca
     8881 cttggcagcg ttattttcca actgccaggt gccttgtttt acagatcgat ctgcattctt
     8941 atattactga ctaaaacttc ccacacctaa acagggcttc gcaccttgaa aattcaatat
     9001 ttcagctaac aaaatagtgc tttatgctat tgcaacctct ggatgaagtg
//
LOCUS       DBMDUA010000110         9038 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3570_length_9038_cov_2.373727, whole genome shotgun sequence.
ACCESSION   DBMDUA010000110 DBMDUA010000000
VERSION     DBMDUA010000110.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9038)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9038
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3570_length_9038_cov_2.373727"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(136..1023)
                     /locus_tag="ACZPCL_08880"
     CDS             complement(136..1023)
                     /locus_tag="ACZPCL_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1162615.1"
                     /translation="MNINLEYYKVFYYVCQEGSLTAAAQRLCISQPAVSQAVRQLEKE
                     AGTKLFSRTSKGVLLTREGELLYHYVKGGVEQLLEGGRMLKRMLDMDMGEVRIGASDM
                     TLQFFLLPYLEQFHKEYPKIKVTVTNAPTPETIRCLEEGKIDFGVVTTPFSGHGAIHS
                     TEVKPIDNVFIAGSSFKELQGKELDYSILCDLPCIFLEKNTSTRLFMDQFLSQKGIEL
                     KPEFELATSDMIVQFARRNMGVGCVMKGFAEEAIERGEVFALSFKEKMPSRSICLVTG
                     ESGLMSMAGKHLLELLTGK"
     gene            complement(1128..2561)
                     /gene="purB"
                     /locus_tag="ACZPCL_08885"
     CDS             complement(1128..2561)
                     /gene="purB"
                     /locus_tag="ACZPCL_08885"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR1162616.1"
                     /translation="MSNDKYVSPLSERYASKEMQYIFSPDMKFKTWRKLWIALAETEK
                     ELGLNITQEQIDELKAHADDINYDVAKAREKEVRHDVMSHVYAYGVQCPKAKGIIHLG
                     ATSCYVGDNTDLIIMTEALQLVRKKLLNVLAELAKFADKYKNQPTLAFTHFQPAQPTT
                     VGKRATLWMMELKLDLDDLEYLIGSMRLLGSKGTTGTQASFLELFDGDHEKCRRLDQR
                     IAEKMGFSGCYPVSGQTYSRKIDSRVLSVLAGIAQSAHKFSNDIRLLQHLKEVEEPFE
                     KHQIGSSAMAYKRNPMRSERIASLSNYVMSDMMNPMLVASTQWFERTLDDSANKRLSV
                     PEGFLAIDGILDLYLNVVDGLVVYPKVIEKHMMAELPFMATENIMMDAVKAGGDRQEL
                     HERIRQLSMEAGRNVKEKGEDNNLLELIAADPAFNLSLEDLKKAMEPSRYVGRAPKQV
                     EEFLEEVINPILKENEEVLGMTAQINV"
     gene            complement(2752..4200)
                     /gene="purF"
                     /locus_tag="ACZPCL_08890"
     CDS             complement(2752..4200)
                     /gene="purF"
                     /locus_tag="ACZPCL_08890"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR1162617.1"
                     /translation="MNQNQMDFDIRGDEVREECGVFGIYDFDGNDVASTIYYGLFALQ
                     HRGQESCGIAVSDTEGPKGKVSAHKGMGLCNEVFTPDVIEPLHGNIGVGHVRYSTAGS
                     STRENAQPLVLNYVKGTLALAHNGNLVNAPELRHELEYTGAIFQTTIDSEVIAYHIAR
                     ARIHTPTVELAVAEAMKKIKGAYSLVIMSPRKLIGARDPFGFKPLCIGKRDNAYILAS
                     ETCALETIGAEFIRDVEPGEIVTITKDGIASNKELCFTDHAKEARCIFEYIYFARPDS
                     VFDGVSVYHSRIQAGRFLAMDSPVDADLVVGVPESGNAAALGYAMESGIPYGTAFVKN
                     SYVGRTFIKPKQSSRESAVRIKLNVLKEAVAGKRVIMIDDSIVRGTTSALIVGMLRDA
                     GAKEVHVRISAPPFLHPCYFGTDIPSEDQLIAHNRTVEEIRELLGADSLSYLALDRLY
                     EMAGGKAICTACFSGDYPIEPPKEDIRGSYEK"
     gene            complement(4358..4729)
                     /locus_tag="ACZPCL_08895"
     CDS             complement(4358..4729)
                     /locus_tag="ACZPCL_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162618.1"
                     /translation="MKKRKAVKQLKKMRKAGVRAWKKKKKKGKMLAFFLFLTPFLFST
                     SATGQENWQGLSVPWPGKEKILEYLIQKEVVREQYVLEKSGKKQDDDTANPVLKEGAA
                     VYLKEGKVLFFKIREEKKQTD"
     gene            complement(4913..5254)
                     /locus_tag="ACZPCL_08900"
     CDS             complement(4913..5254)
                     /locus_tag="ACZPCL_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162619.1"
                     /translation="MKKIRPFLFVCMFVLLSLSLTACAGRKNGNMNGTEQNSATSSAA
                     SETGNGTGAGTGENGMNGDTASGWGEGENGTMTGGTNGEIETTGEGLLDDLTDDLSRG
                     ASDAASDVEGR"
     gene            complement(5337..6095)
                     /gene="dapB"
                     /locus_tag="ACZPCL_08905"
     CDS             complement(5337..6095)
                     /gene="dapB"
                     /locus_tag="ACZPCL_08905"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR1162620.1"
                     /translation="MVRVIMHGCNGVMGQVITGMAKDMEGLTIVAGIDLKNDKENGYP
                     VYASLEECKEEADAVVDFASPKAADHLMDFCAEKKLGLVLCTTGLSEEQIKKVKETAE
                     KTAVLRSANMSLGVNLLLKLVQEAAKVLASSGFDIEIVEKHHNQKKDAPSGTALALAE
                     SMNEALGNQYHYVYDRSSVHEKRDPKEIGIASVRGGSIVGDHDVIFAGKDEVITFNHT
                     AYSKAIFAKGALEAAKFLAGKEPGLYDMSDVVQL"
     gene            complement(6139..7023)
                     /gene="dapA"
                     /locus_tag="ACZPCL_08910"
     CDS             complement(6139..7023)
                     /gene="dapA"
                     /locus_tag="ACZPCL_08910"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR1162621.1"
                     /translation="MAIYEGAGVALITPFKANGEVNYDKLEEIVEEQIAGGTDAIVAC
                     GTTGEASTMTHEEHLDVIKFVCEVTKKRIPVIAGTGSNCTETAIYLSQEAEKNGADGL
                     LVVSPYYNKATQKGLIAHFSAVANSVKIPMLLYNITGRTGVTIQPATIAHLWKNVDNI
                     VGVKEANGDFSATATLMNLTDGGIDLYSGNDDQIVPLLSLGGKGVISVLSNVAPAQAH
                     EICAAYFAGDVKRSAKLQLDAIPLIHALFSEVNPIPVKAAMNLLGKETGPLRMPLTEM
                     EPEHQEVLKKELQAYGLL"
     regulatory      complement(7203..7379)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(7429..7938)
                     /locus_tag="ACZPCL_08915"
     CDS             complement(7429..7938)
                     /locus_tag="ACZPCL_08915"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR1162622.1"
                     /translation="MMKENLIQVICGPGKGKTASALGRGVTALLKGKTVIMVQFLKGS
                     METEGMEIVKRLEPEFKLFRFEKSPIFFDQLSEEEKDEARINIRNGLNFAKKVLVTGE
                     CDILILDEILGILDEGIISLEELKALICQARQSEAELIMTGTVYPQELDEYADEITKV
                     QTRYEHCEG"
     gene            complement(8064..8693)
                     /locus_tag="ACZPCL_08920"
     CDS             complement(8064..8693)
                     /locus_tag="ACZPCL_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR1162623.1"
                     /translation="MTEKMIENNKVSVIGEIVSDFTFSHEVFGEGFYMVELSVNRLSE
                     QADIIPLMVSERLVDVHKDYKGCTMEAVGQFRSYNRHEGTKNRLMLSVFVREIHFMDE
                     FTDYTKTNQIFLDGYICKAPIYRKTPLGREIADLLLAVNRPYGKSDYIPCISWGRNAR
                     FASGFEVGTRVRIWGRVQSREYTKKITETECEKRIAYEVSISKLECDEF"
BASE COUNT         2268 a         2261 c         1707 g         2802 t
ORIGIN      
        1 gggcataagc ccagtatcat aataaataat caatcttctt ttactttact atattatagg
       61 ctataagtaa agtgattatt tattatgaat gtcataatat cagcttatgt tatctgtatc
      121 cgctatctat ttttcttatt tccctgtaag cagttccaac aggtgctttc ccgccatgga
      181 cattaacccc gactctcctg ttaccagaca gatggaacgg gacggcattt tttccttaaa
      241 ggaaagggca aatacttctc cccgctctat tgcttcttct gcaaagcctt tcatcacgca
      301 gccaacgccc atgttccgcc tggcaaactg tacgatcata tcactggtag ccagctcaaa
      361 ttccggcttt aattcaatgc ctttctggga taaaaactga tccataaaca ggcgggtgct
      421 ggtatttttt tcaaggaaga tacaaggaag atcacagaga atggaatagt ccagctcttt
      481 cccctgcaat tctttaaagc tgcttcctgc aataaataca ttatcaatcg gctttacctc
      541 tgtactgtgg atagctccat gacctgaaaa tggcgttgtt accacaccga agtcgatctt
      601 gccctcttcc aggcagcgga tggtctccgg tgtgggcgca ttggtcactg tcaccttgat
      661 cttcggatat tctttgtgga actgctccag ataaggaagt aaaaagaact gcagtgtcat
      721 gtcactggca ccgatccgca cctctcccat atccatatcc agcattcttt taagcatccg
      781 tccgccttct agcagctgtt ctaccccgcc ttttacataa tggtataaca gttctccttc
      841 tcttgtaagg agcacccctt tggaagtacg ggaaaataat tttgttcccg cctctttttc
      901 cagctgacgc actgcctggc ttactgccgg ctgggaaatg caaagtctct gggccgctgc
      961 cgtaaggctg ccttcctgac atacataata aaataccttg tagtattcca gattgatatt
     1021 cataaaattc cccttgcata aagcgcagga tccctgtgct tattaaaagg gggctgaatg
     1081 ctttcgctcc agcccccttc gtccatttat ttcaaattta tgatctttta tacattgatc
     1141 tgagcagtca ttcccagaac ttcttcgttt tcctttaaga tcgggttgat gacttcctct
     1201 aagaactctt ctacctgctt tggtgcgcgt cctacatagc gggatggctc catagccttc
     1261 tttaaatcct ctaaggacag gttgaatgca ggatctgctg cgatcagctc taacagattg
     1321 ttgtcctcac ccttttcttt tacgtttctg cctgcttcca tggaaagctg acggatacgc
     1381 tcatgaagtt cctgacggtc tccacctgcc tttacagcat ccatcataat gttttctgtt
     1441 gccataaatg gaagctctgc catcatgtgc ttctcaatta cctttggata tactaccagt
     1501 ccgtctacta cgtttaagta cagatccagg ataccatcga tagctaaaaa tccttccggt
     1561 acggacagtc tcttatttgc agagtcatcc agagtacgct caaaccactg ggtagaagca
     1621 actaacattg gattcatcat atcgctcatt acgtagttgg acagggaagc aatgcgctcg
     1681 ctgcgcatcg ggtttctctt gtatgccata gcggaagaac cgatctggtg cttttcaaat
     1741 ggctcttcta cttcttttaa gtgctgcaga aggcggatat cattggagaa cttgtgagca
     1801 ctctgtgcga tacctgctaa aacgctgagc acacggctgt cgatctttct ggaataggtc
     1861 tgtccggata caggatagca gccggaaaat cccattttct ctgcgatccg ctgatctaaa
     1921 cgacggcatt tttcatgatc tccgtcaaac agctccaaga aacttgcctg ggttcctgtg
     1981 gttcccttag aacctaacag acgcatagaa ccgatgagat attccagatc gtctaaatcc
     2041 agctttaatt ccatcatcca taaggttgct ctcttaccta cagttgtagg ctgtgcaggc
     2101 tggaaatggg taaatgccag tgttggctga tttttgtact tatctgcaaa tttagccagt
     2161 tcagccagta cgtttaataa tttcttgcgg actaactgca gagcctcagt cataatgatc
     2221 agatctgtat tgtctcctac ataacaggag gtagcgccta aatggataat gccttttgcc
     2281 ttcgggcact gtacgccgta tgcgtacaca tggctcataa catcgtgacg tacttctttt
     2341 tctcttgcct ttgcaacatc ataattgatg tcatctgcat gggctttcag ctcgtcgatc
     2401 tgctcctgtg taatatttaa tcccagttct ttctctgtct cggccagcgc gatccagagt
     2461 tttctccagg tcttgaattt catatccggt gaaaaaatgt actgcatctc tttgcttgca
     2521 taacgctcag aaagagggct tacgtattta tcattactca ttggtttttc ctcctgtgcc
     2581 tgcttagtag gcttttttta ctcatgaacc ccgcgctaac gcgctctact gtctgctccg
     2641 cagacggttc attcgttaac ggtttgtgaa aaacaaatgt ctgcttcgca gccgcttgtt
     2701 ttcccctgca ctcgttacag tataacatat ctgcgttttc taaacaacca tctacttctc
     2761 atagctgccg cggatatctt cttttggcgg ctcgatcgga taatccccgc tgaaacatgc
     2821 agtacagatc gcctttcctc ctgccatttc atacagacgg tccaaagcca gataggataa
     2881 gctgtctgcc cctaaaagtt cacggatctc ttctactgta cggttatgag cgatcagctg
     2941 gtcttctgac gggatatcgg ttccgaagta acatggatga aggaatggcg gcgcactgat
     3001 gcgcacatgg acttctttgg ctcctgcgtc tcttaacatg cctacgatca gggcactggt
     3061 agttccacgg acaatagagt catcgatcat gatcacacgt tttcctgcca cagcttcttt
     3121 taatacattt aatttaatgc ggaccgctga ttcacggcta ctctgtttcg gcttgataaa
     3181 agttcttcct acataggaat tctttacaaa agcagttcca taaggaatac ctgactccat
     3241 tgcatagcca agggctgctg catttccgga ctccggaacg cccactacca gatctgcatc
     3301 taccggtgag tccattgcca gaaaacggcc tgcctgaata cgggaatgat atacgcttac
     3361 accgtcaaat acactgtccg gtcttgcaaa ataaatatat tcaaaaatac atcttgcttc
     3421 ttttgcatga tctgtaaagc aaagttcttt attagaagca atgccgtcct ttgtaatggt
     3481 aacgatctca cctggctcta cgtcacggat aaattctgca ccgatagtct ctaaagcaca
     3541 ggtctcagat gccagaatat aggcattatc tctctttccg atgcagagag gcttaaatcc
     3601 aaatggatct ctggctccga tcagctttct tgggctcatg atcaccaggg aatatgctcc
     3661 cttgatcttt ttcatagctt ctgccactgc cagctctacg gttggtgtat ggatacgcgc
     3721 ccttgcaatg tgatacgcaa tgacctcaga gtcaatggta gtctggaaaa tagcgcctgt
     3781 gtattctaat tcatgacgca gctccggtgc attgaccagg tttccgttgt gagctaacgc
     3841 cagggttcct tttacatagt ttaataccag aggctgcgca ttctctctgg tactgcttcc
     3901 tgctgttgaa tatcttacat ggcctacacc gatatttcca tgaagtggtt cgatcacatc
     3961 cggtgtaaat acttcgttgc aaagacccat tcccttatgc gcacttactt ttccttttgg
     4021 gccttctgta tcactgactg caataccgca gctttcctgt cctctgtgct gaagagcaaa
     4081 caggccataa taaattgtgg aggcaacatc atttccgtca aaatcataaa tgccgaaaac
     4141 accacattcc tcccgcactt catctcctct gatatcaaag tccatctgat tctggttcat
     4201 tggtctttcc tttccgcttt cgccttttat cacagtcttg tcatttataa gtcataacga
     4261 taaaagaagt cattttcagt agaaattata atacacgttt tctgtgaata caagcaacag
     4321 gctcattaaa attcctaatg aataaattcc ctctgttcta atcagtctgt tttttctcct
     4381 cccggatctt gaaaaataac acttttcctt ctttcagata aacagcagcg ccttctttta
     4441 aaaccggatt tgctgtatca tcatcctgtt tttttccaga tttttccaat acatattgtt
     4501 cccggactac ttctttttgg atcaggtatt ccaggatctt ctcttttccc ggccatggca
     4561 cagaaagccc ctgccagttc tcctgccctg tggcagaggt tgaaaacaga aaaggtgtca
     4621 gaaataaaaa gaaagctaac attttacctt tctttttctt ctttttccag gctctcaccc
     4681 ctgcctttcg cattttcttt aattgtttca ctgcttttct ttttttcatg ggagtccttt
     4741 cccacgcact ccagttttat tatagaaaat ttttatcgtt ccttcaagag taaaacaggc
     4801 tgtaaaaaaa agcaaaaaac tgccaggaaa aagaattttc atctttctcc tgacagtcat
     4861 cactgcagcg catatgacaa gtgtaccttt gcacgctcat tgatcacata agttacctgc
     4921 cttccacatc acttgctgca tcggaagcgc cacggctcag atcatctgta agatcatcga
     4981 gcagcccttc tccggtagtc tcgatctctc cattggtgcc gccggtcatg gtgccatttt
     5041 ctccttcgcc ccagccggat gcggtatctc cattcatgcc attttccccg gttcctgctc
     5101 ctgttccatt tccagtctca gaagccgccg aggaagtagc actgttctgc tccgtcccat
     5161 tcatattgcc attttttctt cctgcacagg cagtaaggga aagagacaaa agcacaaaca
     5221 tgcatacaaa taaaaacggt ctgatctttt tcataaaaca ttctcctttc gtttacctgc
     5281 tattagtatg taacatcaga gaatgcttta tagtgttaaa tttttccaga caaaatctac
     5341 agctgcacta cgtcactcat gtcgtataat ccaggttctt ttccagctaa aaattttgct
     5401 gcttccagag cgccttttgc gaaaatagcc ttggaatatg ctgtatgatt aaaggtaatg
     5461 acctcatctt ttccggcaaa gatcacatca tgatctccta caatagaacc gccgcggaca
     5521 gatgcaatgc cgatctcctt tggatctctc ttttcatgaa cgctgctgcg gtcatataca
     5581 tagtgatact gattgcctaa agcttcgttc atagactcag ccagtgcaag ggcagttccg
     5641 cttggagcat cctttttctg attgtggtgt ttttccacga tctcaatatc aaaaccggaa
     5701 ctagccagta ctttcgctgc ctcctggacc agttttaata aaaggtttac gcctaaagac
     5761 atattggcag aacgcagtac tgctgttttt tctgctgtct cttttacttt tttgatctgc
     5821 tcttcagaaa gcccggtggt acaaagtacc agtcccagct tcttttctgc acagaaatcc
     5881 ataaggtggt ctgctgcctt tggagaagca aaatctacta ctgcatctgc ctcttctttg
     5941 cattcctcta aggatgcata gaccggatat ccattttctt tatcattttt aagatcaatg
     6001 cctgcaacaa tggtaagccc ttccatatcc tttgccattc cggtgatgac ctggcccata
     6061 acaccgttgc agccatgcat gatgactctg accatttttc tttcctttct cctgctgccc
     6121 taaagaatag ttttctcctt acagcagtcc gtatgcctgt aattccttct taagaacttc
     6181 ctgatgctca ggttccatct cagtaagagg cattcttaaa ggtcctgttt cttttcctaa
     6241 caggttcata gctgccttaa cagggatcgg gtttacctcg ctgaataaag catggatcag
     6301 tgggatcgca tctaactgaa gctttgcact gcgctttaca tcaccggcaa aatatgctgc
     6361 acagatctca tgtgcctgag caggagctac atttgataat acagagataa cgccctttcc
     6421 gcctaaggat aataacggaa cgatctggtc atcatttccg gagtacaggt ctataccgcc
     6481 gtctgttaag ttcattaaag ttgcagtagc agagaaatca ccattagcct cttttacgcc
     6541 aacgatatta tctacatttt tccaaagatg agcgatggta gcaggctgga tggtcacacc
     6601 ggttcttccg gtgatattgt aaagaagcat tgggatcttt acggaatttg caactgcgga
     6661 aaaatgtgcg atcagaccct tctgggttgc cttattgtaa tatggagata ctactaacag
     6721 accgtcagca ccattcttct ctgcttcctg ggacagataa atagcagtct ctgtgcagtt
     6781 agaacctgta ccagcaatta caggaatacg cttctttgta acttcacata caaacttgat
     6841 aacgtctaag tgctcctcat gggtcatggt agaagcttca cctgttgtac cgcaggcaac
     6901 aatggcatct gtacctcctg cgatctgctc ttctacgatc tcctctaact tgtcgtaatt
     6961 aacctctcca tttgccttaa atggtgtgat cagtgcaaca ccggcgcctt cataaattgc
     7021 catgtctttt ctcctcctaa atatagtaaa attgttggaa acacgcagta cgcgtccccg
     7081 gatccaccag agtgttccta tcgcgatgtg atctggaaaa atctggtgat cacatcaaaa
     7141 gaaaaggaag ggctgaggta aatgtcagct cttccaacgg tttcgcatct tcacgaatct
     7201 gctctttcgg tagctctcca tccacccgga tgacagtcac aaagttctta accctgtgcc
     7261 cagaaaagac tctttcaggt ttcctctttt cttcggcgct tttccctttc accagacctt
     7321 cttctgtgcc tgtcacatcc ggccggttac ttttgatcag cgcaacctct actctgtttt
     7381 atgttcttaa tattagcatt atcagttgca gatgtcaact gtaaatattt aaccctcaca
     7441 gtgttcataa cgtgtctgaa ccttggtgat ctcgtctgcg tactcatcta attcctgagg
     7501 ataaacggtt cctgtcatga tcagttctgc ttcggactgt cttgcctgac agatcagcgc
     7561 ctttaactcc tccaatgaaa taatgccttc atccaggatc cccaatatct catccaggat
     7621 caggatatcg cactctcctg taaccagtac cttttttgcg aaattcaaac cattgcggat
     7681 attgatcctt gcttcgtcct tttcttcctc tgaaagctgg tcaaaaaaga tgggtgattt
     7741 ttcaaatcgg aataatttga actccggttc cagtcgtttt acgatctcca taccttctgt
     7801 ttccatggaa ccctttaaaa actgtaccat gatgactgtt ttccctttta aaagagcggt
     7861 cacacctcgt cctaaggcac ttgcggtctt tccttttcca ggtccgcaga tcacctgtat
     7921 cagattttct ttcatcattc tgcacctcat gtctgttctc tcatgtgagc atttttcaaa
     7981 accactctag cacaatttat gtaataatgc aaatatttct tacgatttcc agaagatttt
     8041 cctgcctaaa tatgaaaatt tatttaaaat tcatcacact ccagcttgct gatagatact
     8101 tcataagcaa tacgcttctc acattctgtc tccgtgatct tctttgtata ctcccggctc
     8161 tggactcttc cccagatacg gacccttgtt ccaacctcaa agccagaagc aaaacgggca
     8221 ttacggcccc agctgataca agggatataa tctgattttc cataaggacg gtttacagct
     8281 aaaagaaggt ctgcgatctc tcttccaaga ggtgttttgc ggtagatcgg cgccttgcag
     8341 atatagccgt ctaagaagat ctggttcgtc tttgtgtaat cggtaaattc atccataaaa
     8401 tggatctcac gtacaaatac cgataacatc aggcggtttt tagtaccttc gtgacggtta
     8461 taggaacgga actggccaac ggcttccatg gtacagccct tatagtcctt atgtacatcc
     8521 acaagccgct cagaaaccat taaaggaatg atatccgcct gttcacttaa acggttgaca
     8581 gataattcta ccatataaaa gccttctcca aagacctcat ggctgaatgt gaaatctgat
     8641 acgatctctc cgatcacact taccttgttg ttttcgatca ttttttctgt catagtactt
     8701 ctcctcttct tttccatttt tattttatga taccagggtc aaaaggtggg aaatccgacg
     8761 taagcttgct tgcaggagga tttatcaact tgggacccgc caaaaacatg agtaagaagc
     8821 cattttttga agaaaaatga gcggattacg aatgttttta ggattgcgtg agtgcaacgc
     8881 acgtagcaat ccggtatctt gggggccatg tacctttgga cacctacata gataatttat
     8941 gaaaagatga aaagaaatag accggatatg cataaaaatc cacctctgtt ttccgaaact
     9001 catccgcact taacttacct tcttccagtt cttgaaag
//
LOCUS       DBMDUA010000111         9021 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3575_length_9021_cov_2.743946, whole genome shotgun sequence.
ACCESSION   DBMDUA010000111 DBMDUA010000000
VERSION     DBMDUA010000111.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9021)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9021
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3575_length_9021_cov_2.743946"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            54..242
                     /locus_tag="ACZPCL_08925"
                     /pseudo
     CDS             54..242
                     /locus_tag="ACZPCL_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
     gene            279..1040
                     /gene="hisF"
                     /locus_tag="ACZPCL_08930"
     CDS             279..1040
                     /gene="hisF"
                     /locus_tag="ACZPCL_08930"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR1162624.1"
                     /translation="MFTKRIIPCLDVNNGRVVKGVNFVNLKDAGDPVEIAAAYDRAGA
                     DELIFLDITASSDHRKTVVDMVRQVAEKVFIPFTVGGGIRTVEDFRLLLREGADKISI
                     NSSAIDNPQLIHDAAQKFGRQCVVVAVDAKRRADGSGWNVYKHGGRLDTGLDVLEWVK
                     KADSLGAGEILLTSMDCDGTKAGYDNELTAAVADLVSIPVIASGGAGTKEHFYDALTK
                     GKADAVLAASLFHYKELEICDLKDYLAGRGISVRR"
     gene            1127..1807
                     /locus_tag="ACZPCL_08935"
     CDS             1127..1807
                     /locus_tag="ACZPCL_08935"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR1162625.1"
                     /translation="MAAISNDWLEPLQGEFKKPYYKKLYDTVLHEYQTRKIFPAPDDI
                     FNAFSFTPLANVKVVILGQDPYHNDGQAHGLSFSVKPDIDIPPSLVNIYQELHDDLGC
                     YIPNNGYLKKWADQGVMLLNTVLTVRAHQANSHHDIGWEQFTDAAIRVLNEQDRPMVF
                     ILWGRPAQAKKAMLNNPKHMILEAPHPSPLSAYRGFFGSRPFSKTNDFLREHGLEPID
                     WQIENIEK"
     gene            1843..2685
                     /locus_tag="ACZPCL_08940"
     CDS             1843..2685
                     /locus_tag="ACZPCL_08940"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR1162626.1"
                     /translation="MSIVEILKVIVLGIVEGFTEWLPISSTGHMILVDEIIHLNQPDA
                     FKDMFLVVIQLGAILAVAVMYFQKLNPFSPSKSARQKQATWALWIKIVIACIPAAVLG
                     LLFDDWMEAHLYNAYVVAATLIIYGVLFIALENSSITANPSITKVGHVSTKTALFIGL
                     FQLLSLVPGTSRSGSTILGAMVLGCSRGVAAEFSFFLGIPVMFGASFLKLVKFFVKGN
                     VLTGPQIFYTILGMAIAFGVSVYSIQFLMGYVRRHDFKFFGYYRIILGVVVLAYFGIT
                     ALAA"
     gene            complement(2797..3828)
                     /locus_tag="ACZPCL_08945"
     CDS             complement(2797..3828)
                     /locus_tag="ACZPCL_08945"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR1162627.1"
                     /translation="MSFKLVNELPSPAEILEQFPLPEKLAVLKAERDEEIKKVITGES
                     NKFLVIIGPCSADNEDAVCDYANRLAKVQEKTKDRLLIVPRVYTNKPRTTGEGYMGML
                     HQPDPEKKPDMLQGILAIRHMHMKVFRETGMSTADEMLYPDNLQYLSDIMSYIAVGAR
                     SVENQYHRLVASGCDVPVGMKNPTSGDLSVMLNSVVAAQLPHDFIFRGDEVSCPGNPL
                     AHTILRGAVNKHGQCIPNYHYEDLHLLFELYSKRNLKNPACIVDTNHSNSNKKYAEQV
                     RIAKEVLHSRRHSDDIRHLVKGLMIESYIEPGNHKIGEHCYGQSITDPCLGWDESEKL
                     LYDIAEMAD"
     gene            4351..5577
                     /gene="tyrS"
                     /locus_tag="ACZPCL_08950"
     CDS             4351..5577
                     /gene="tyrS"
                     /locus_tag="ACZPCL_08950"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR1162628.1"
                     /translation="MQIYEELVARGLIAQVTNEEEIRKMVNEGKAVFYIGFDPTADSL
                     HVGHFMALCLMKRLQMAGNKPIALIGGGTGMVGDPSGRTDMRKMMTPEIIQHNCDCFK
                     KQMSRFIDFSDGKALMVNNAEWLMNLNYIEFLREVGPHFSVNNMLRAECYKQRMEKGL
                     SFLEFNYMLMQSYDFYELFQRYGCNMQFGGDDQWSNMLGGTELIRRKLGKDAHAMTIT
                     LLLNSEGKKMGKTQSGAVWLDPEKTSPFDFYQYWRNVGDADVLKCLRMLTFLPLEQID
                     EMDKWEGSQLNKAKEILAFELTKLVHGEEEATKAQEGARALFAGGANTDNMPTCELSE
                     EDFADDNIDILTVLTKSGLAASRSEARRNVEQGGVSVDGTQIKDIKAVFAKDQFAGDG
                     VVVKRGKKNFKKVILK"
     gene            5601..6332
                     /locus_tag="ACZPCL_08955"
     CDS             5601..6332
                     /locus_tag="ACZPCL_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007036978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162629.1"
                     /translation="MKKCVTLLLAAVLAVGSAFSSQAGSAFKKEVPLTLHMDINNRQT
                     PIYDGYTSYIMGNRASEKDTFTVTKNSDTLNLDDVTLSYYLVTYDGGTEKNLECRVYG
                     LKEGEHYPVVRPETFSRESEKGDVYDSLERCCVVEFSKDDERKEYYFTMAPETDMAAY
                     RNIHLGKWEKDVKGWRYQYQGEYLTSWAKINDKWYLFGMDGYMLTGWQDSMGALYYLN
                     PSDGVMMTNCTIDGRRLDGSGAYRE"
     gene            6329..7681
                     /locus_tag="ACZPCL_08960"
     CDS             6329..7681
                     /locus_tag="ACZPCL_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1162630.1"
                     /translation="MKKSYEIDMCNGPLLGKILVFSIPLMLSGILQLLFNAADIIVVG
                     RFAGSTALAAVGSTASLINLMINVFVGLSVGVNVLAARYYGAQKEKDIQDTVHTAIMM
                     AILSGLFLVVLGESASKPMLTLMGTPDDVLDQAALYLRIYFFGMPVLMIYNFGAALLR
                     AVGDTRRPLYFLFAAGAVNVVLNLFFVIQLGMGVDGVAWATVISECVSAGLIIRSLMK
                     ENGAMKLHLTKLRIVSGKLTQIVRVGLPAGMQGAIFSISNVLIQSSVNSFGSIAMAGN
                     TASSNIEGFVYTAMNAVYQTSLSFTSQNLGGEKFSRIDKIKNICLGVVTVVGLVLGWG
                     AVLGGHQLLGIYSSDPEVLSFGMRRLRIICGTYFLCGLMDCMVGSLRGMGYSVIPMFV
                     SLTGACAFRVFWIFTIFAVNRTLEVLYLSYPVSWIITAASHMVTYYIVRKHFPKEDKA
                     "
     gene            7834..8583
                     /locus_tag="ACZPCL_08965"
     CDS             7834..8583
                     /locus_tag="ACZPCL_08965"
                     /inference="COORDINATES: protein motif:HMM:NF019349.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR1162631.1"
                     /translation="MKNNNANRMILDDMKAKILRGEYPVGSKLPSERELSEYYNVSRI
                     PVREALKALSDMGILEIKRGAGTFVKSTANAMQNGEKEQTETFMQPDVILLETIHVRK
                     LLESEAAAQAAKNIRKDEIDDLEKALITTIQEIRKLKTGQDNDFFQADAKFHRLILRA
                     SHNPVFEQCLEAMPNIMSLHQYWSLKMTTPMDEVVTYHCSIFENILIHNEAGAREAMM
                     AHLSRVEELLARKVPEEKEGESPVPKVWYTT"
BASE COUNT         2658 a         1742 c         2178 g         2443 t
ORIGIN      
        1 aaggccgctg acggaggaga gtatcagggt gtttaaggaa aattgcggga agctatgtat
       61 attttgtcca ttcctattat ctgaaagcag gatctgaaga tatagtggca gcaacaacag
      121 agtatggaac ccatatccat gcagcggtgg aaaaaggtaa tctttatgcc tgccagttcc
      181 atccggaaaa gagcagtcag acaggactta agatccttga gaattttatc agcctgccat
      241 aaagatttta aaagtacgca gagaaaggaa gagaaaatat gtttacaaaa aggatcattc
      301 cctgcctgga tgtgaataat ggccgggtgg taaaaggtgt taattttgta aatctgaaag
      361 atgccggtga tccggtagag attgcagctg cttatgacag agccggtgca gatgaactta
      421 tatttctgga tattacagcg tcttctgacc atagaaaaac agtagtggat atggtgcgtc
      481 aggtagcaga aaaggtattt atccctttta cagtaggcgg cggtatccgt acagtagaag
      541 atttcagact gcttcttcgt gaaggcgcag ataagatatc cattaattca tctgctattg
      601 acaatccaca gctgatccat gatgcagccc agaaattcgg cagacagtgc gtcgtggttg
      661 ctgttgacgc aaagcgcagg gcagacggtt ccggttggaa tgtatacaaa cacggtggac
      721 gcttagatac aggtctggat gtgctggagt gggtaaaaaa agcagactct ttaggagcag
      781 gagagattct tcttaccagt atggactgtg atggcacaaa agccggttat gacaatgaac
      841 taactgcagc tgtagcagat ctggtcagta ttcctgtgat cgcttccggc ggagctggaa
      901 caaaagagca tttttatgat gcactgacta agggaaaggc agatgcagtg ctggcagcgt
      961 cattattcca ctataaagaa ctggagatct gtgatctgaa ggattatctg gctggcagag
     1021 gtatttctgt gcgcagataa gattatctgt tgtgtagata aaaatattgg cattaacaga
     1081 taaaagactg tgaaaaatac aacatcacag aagaaaagga gcaaaaatgg ctgcaatatc
     1141 caatgactgg cttgaaccat tgcagggtga atttaaaaag ccatattata aaaaattata
     1201 tgataccgta cttcacgaat atcagacccg caagatattt cctgcgccgg atgatatttt
     1261 caatgcattt tcttttacac cattggcaaa tgtaaaagtc gtgatccttg gccaggatcc
     1321 ataccacaac gacggccagg cacatggact gagtttttcc gtaaagccgg atatagatat
     1381 ccctccgtcc ctggtgaata tttatcagga acttcacgat gatcttggct gttacatacc
     1441 aaataacggc tatctgaaaa aatgggcgga ccagggcgtt atgcttttga acactgttct
     1501 tacagtacgt gcccatcagg ccaattccca tcatgatatt ggctgggagc agtttacaga
     1561 cgctgccatc cgggtcttaa atgaacagga ccgtccgatg gtatttatcc tgtggggcag
     1621 accggcccag gcgaaaaagg ctatgttaaa caatccaaag catatgatct tagaggcgcc
     1681 tcatccaagc ccgttatctg cttacagagg atttttcggc agccgtcctt tcagcaagac
     1741 caatgatttt ctcagggaac atggtttgga gccgattgac tggcagattg aaaacataga
     1801 aaaataaata taaaaataaa gataaaaata taaggagtaa taatgagtat agtagaaatt
     1861 ttaaaggtga tcgttctggg aattgtggaa ggtttcaccg aatggctgcc tatcagcagc
     1921 acaggtcata tgatcctggt agacgagatc atccatttaa accagccgga cgcatttaaa
     1981 gacatgtttc tggtagtgat ccagttaggt gccatccttg cagttgcggt catgtatttc
     2041 cagaaattaa acccattcag cccatctaaa tcagccagac agaagcaggc aacctgggca
     2101 ttgtggatca agattgtgat cgcatgtatc ccggcagctg tattgggcct tttatttgat
     2161 gactggatgg aagctcatct gtataacgca tatgtagtag cagctacact gatcatttat
     2221 ggtgtgctgt tcattgctct ggaaaacagc tctattacag ctaacccatc cattaccaaa
     2281 gttggtcatg tttccactaa aacagctctt tttattggtc tgttccagct tctttccctg
     2341 gttccgggaa cttcccgttc cggttctacc attttaggag caatggtact tggctgttcc
     2401 agaggcgtgg cagcagaatt ttccttcttc ttaggtattc cggtcatgtt tggagcaagt
     2461 tttttaaaat tagtgaagtt ttttgtaaaa ggaaatgtat taactggtcc tcagatcttt
     2521 tacaccattc tgggaatggc tattgctttt ggcgtatctg tatattccat ccagttctta
     2581 atgggatatg tgcgcagaca cgactttaag ttctttggat actatcgtat tatcttaggc
     2641 gttgtagtgc ttgcgtattt tggaattaca gcactggctg cctgaaaaat ataaatcccc
     2701 aaatgcctac atggcaggca gataaaagat cgcaaacaaa aaactccgtc ctgttatcat
     2761 atcaggaacg gagtttttta ataccgatct taatttttaa tcagccatct cagcaatatc
     2821 atacagcagc ttctcagact catcccatcc aagacatggg tcagtaatgg actgtccgta
     2881 gcagtgttcg ccgatcttgt ggtttcctgg ctcgatgtag ctttcgatca tcaggccttt
     2941 taccagatga cggatgtcat cagaatggcg gcggctgtga agcacttctt ttgcgatacg
     3001 aacctgttct gcatacttct tgttggagtt ggagtggttg gtatctacga tgcaggccgg
     3061 gttctttaag tttctcttgg agtaaagctc aaagagcagg tgcaggtcct cataatggta
     3121 gttcgggatg cactgcccat gtttattgac agcaccgcgc aggatggtat gggctaatgg
     3181 atttccaggg caggaaactt catcaccgcg gaagataaag tcatgtggca gctgggctgc
     3241 tacaacagag tttaacataa cgctgagatc gccgctggta gggtttttca taccaaccgg
     3301 tacatcacag ccgctggcta ccagacggtg gtactggttc tctacagaac gcgcgccaac
     3361 tgcaatataa gacatgatat cagacagata ctgaaggtta tccggataca gcatttcgtc
     3421 agcagtagac attccggttt cacggaatac cttcatgtgc atatgacgga tcgctaaaat
     3481 tccctgaagc atatcaggct ttttttccgg gtccggctga tggagcatac ccatatagcc
     3541 ttcgcctgtg gttcttggct tattggtata aactcttggt acaatgagaa gtctgtcttt
     3601 tgtcttttcc tgaacctttg caagacggtt tgcatagtcg cagacagcat cttcattatc
     3661 tgcggaacaa ggtccgatga taacaaggaa tttgttgctt tctccggtaa ttactttttt
     3721 gatctcttca tctctttcag cttttaaaac tgccagcttc tcaggcagag ggaactgttc
     3781 taagatctct gccgggcttg gaagttcatt tacaagtttg aaactcatgt cttctttatc
     3841 ctcccgatgt aacaacttat gaaaacaaat gtctgccggt gcagtcactt ctttttcttt
     3901 aggactcatt atagtataac tttgtggaag ttgcaacgca aaaaaatccc attgacaaaa
     3961 atgatataac tgcctataat agccttaatt tcatgacttg gcaatgatgg gaacaagtaa
     4021 ttttctgcaa acgcatacag aaagcagccg gcagatgaga ggcgcatgga aagcagaaca
     4081 tgaatacatc cctgagcttt gcaccgaata acataaaagt ttagtaggct gcaacggaga
     4141 tggtacacgt tatagtaccc gggtatcctc gaaagagcgt accttttaag gcaggcagtg
     4201 tgagctgtct gtgaattaag gtggtaacac gggcttacag cttcgtcctt tagcggggac
     4261 gaggcttttt ttattccctg ttccttgtac atgaaagaaa aaattgctat acttagaatt
     4321 aagatataat gcgacgaaag gaaagaaaaa atgcagatct acgaagagtt agttgccaga
     4381 ggcctgatcg cccaggtaac aaatgaagaa gaaatccgta aaatggtaaa cgaaggaaag
     4441 gcagttttct acatcggttt tgacccaacc gctgacagcc ttcatgtagg acatttcatg
     4501 gcactgtgcc tgatgaaacg tctccagatg gcaggaaata agccgatcgc cctgatcggc
     4561 ggcggaaccg gtatggtagg tgacccatcc ggacgtacag atatgcgtaa gatgatgact
     4621 cctgagatca tccagcacaa ctgtgactgc ttcaagaagc agatgagccg ttttatcgac
     4681 ttctccgatg gaaaagccct gatggtaaac aatgctgagt ggctgatgaa cttaaactac
     4741 atcgaattct taagagaagt aggacctcat ttctctgtaa ataacatgct ccgtgcagag
     4801 tgctacaagc agagaatgga aaaaggttta agcttcttag agttcaacta catgctgatg
     4861 cagagctacg acttctatga gctgttccag cgctacggct gcaacatgca gtttggtggt
     4921 gatgaccagt ggagcaatat gttaggcggt accgagctga tccgtcgtaa actgggcaaa
     4981 gacgctcatg ctatgaccat taccctgtta ttaaactccg aaggcaagaa gatgggcaag
     5041 acccagtccg gcgctgtatg gctggatcct gaaaagacat ctccatttga cttctatcag
     5101 tactggagaa atgttggtga tgctgacgtt cttaagtgcc tgcgtatgct taccttcctg
     5161 ccattagagc agatcgatga gatggacaag tgggaaggaa gccagttaaa taaggcaaaa
     5221 gaaatccttg catttgagct gaccaagtta gttcatggtg aagaagaggc aacaaaggca
     5281 caggaaggcg caagagcttt atttgcaggc ggcgctaata cagataatat gccaacctgc
     5341 gagttaagtg aagaagattt cgcagatgac aatattgaca ttttaaccgt tcttacaaag
     5401 tccggcctgg ctgcatcccg ttctgaggca agaagaaacg tagagcaggg cggtgtatct
     5461 gttgacggaa ctcagatcaa ggatatcaag gcagtatttg caaaagacca gtttgcaggt
     5521 gacggcgttg tagttaagcg cggaaagaag aactttaaga aagttatttt aaagtaggac
     5581 agatgagagg aggatgttaa gtgaaaaaat gcgtgacatt gcttctggca gcagttctgg
     5641 ctgtcggaag cgccttctct tctcaggctg gctctgcctt taaaaaagaa gttcccctta
     5701 ccctgcatat ggatatcaat aacaggcaga ctcctattta tgatggatat acttcctata
     5761 taatgggaaa ccgggcatct gaaaaagata catttaccgt tacaaaaaat tcagacacct
     5821 taaacctgga tgatgtgacc ttaagctatt acctggttac ttatgacggc ggaacagaga
     5881 aaaatctgga atgccgtgta tatggtctga aagaaggaga acattatccg gtggtccgcc
     5941 cggaaacatt tagccgtgaa agcgaaaaag gagatgttta cgactccctg gaacgctgtt
     6001 gcgtggtgga attttccaag gatgatgagc gcaaagaata ttattttacc atggctccgg
     6061 aaacagatat ggctgcttac cgtaatatcc atctgggaaa atgggaaaaa gatgtaaaag
     6121 gatggcgcta ccagtatcag ggagagtacc ttacatcctg ggcaaagatc aatgataagt
     6181 ggtatctttt tggcatggat ggatatatgt tgacaggctg gcaggactct atgggagcct
     6241 tgtattatct gaacccttca gacggcgtga tgatgacaaa ctgtaccata gatggccgca
     6301 ggctagatgg gagcggagca tacagagaat gaaaaaatca tatgaaatag acatgtgtaa
     6361 cggccctctt ctgggcaaga tccttgtttt ttcaattccc cttatgcttt caggtatcct
     6421 tcagctgctt tttaatgcag cggatatcat tgtagtagga agatttgcgg gaagtacagc
     6481 gttagcagca gttggctcta cggcatcttt gatcaatctg atgatcaatg tgtttgtagg
     6541 gctttctgtg ggtgttaacg ttttagcagc cagatattat ggcgcacaga aagaaaaaga
     6601 tatccaggat acggtacaca cagcgatcat gatggctata ttaagcggtc tgttcctggt
     6661 agttcttgga gaaagtgctt caaagcctat gctcacactc atgggaacac cggatgatgt
     6721 actggatcag gcggcactgt atctgcggat ctattttttt ggaatgccgg tgcttatgat
     6781 ctataacttt ggcgcagctc ttttaagggc agtgggggat accagacggc ctctgtactt
     6841 tctgtttgcg gccggtgcag taaatgtagt gttaaatctg ttttttgtga tccagctggg
     6901 aatgggagta gacggtgttg cctgggctac tgttatttct gaatgtgtat cagcaggtct
     6961 tattatccgc agtctgatga aggaaaacgg agccatgaag ctgcatctta caaaacttcg
     7021 tattgtatcc ggaaagctga cacagattgt gcgtgtaggt cttccggcag gtatgcaggg
     7081 agcgatcttt tccatatcca atgtgctgat ccagtcatct gttaactctt ttggttccat
     7141 tgccatggcg ggaaatacgg cttcttccaa tattgaaggc tttgtataca cagccatgaa
     7201 tgcggtgtat cagaccagtt tgagttttac cagccagaac ctgggcggtg aaaaattcag
     7261 ccgtattgat aagataaaaa atatctgtct tggagttgtt actgtagttg gactggtact
     7321 gggatggggc gcagttcttg gcggccatca gctgttgggt atctattcct ctgatccgga
     7381 agttttaagc tttggtatgc gaagactgcg gatcatctgc ggtacctatt tcctttgcgg
     7441 tcttatggac tgtatggtag gaagtttaag aggaatggga tattctgtga tccccatgtt
     7501 tgtatccctt acaggagcat gcgcattccg tgtattctgg atctttacaa tctttgcggt
     7561 caaccgcacg ctggaagtgc tgtacctgtc ctatccggtt tcctggatca taacagcagc
     7621 atcccatatg gttacatatt atatagtacg aaaacatttc ccgaaagaag ataaggctta
     7681 gttactgggc attagatcct ttcgggatct ttttatgtct ggatttgcta aaagaaatag
     7741 aaaatgactg gtatgttttg gtatcttcct gattgcatga aattggtctg actggtataa
     7801 tactaggtat caaactggag aaaattataa aatatgaaaa ataataatgc aaacagaatg
     7861 atcctggatg atatgaaggc caagatctta agaggagaat atcccgttgg cagtaaactg
     7921 ccttctgaac gtgaactttc agaatactac aatgtaagcc gcattccggt aagagaagcg
     7981 ttaaaagctc tttctgatat gggaatatta gagatcaagc ggggagccgg aacctttgta
     8041 aaaagtacgg caaacgctat gcagaatgga gagaaagaac agacagaaac ctttatgcag
     8101 ccggatgtga tccttcttga aaccattcat gtaagaaaac tgctggagtc agaagcggca
     8161 gcccaggcgg caaaaaatat ccgtaaagat gagatcgatg atctggaaaa ggcattgatc
     8221 accacaatcc aggagatccg gaaactgaaa accggtcagg acaatgattt ctttcaggca
     8281 gatgcgaagt ttcaccgtct gatcctgcgg gcttcccata atcctgtatt tgagcagtgc
     8341 ctggaagcaa tgcctaatat tatgtccctt catcagtact ggagtttaaa aatgactacg
     8401 cctatggatg aagtagttac ttatcactgc agtatttttg aaaatatcct gatccacaat
     8461 gaagcaggag caagagaagc tatgatggct catttaagca gagtagaaga actgctggca
     8521 aggaaagtac cagaagaaaa agaaggggaa agtccggtgc cgaaggtgtg gtacaccact
     8581 tgatacggaa atagtatgac agaaaaataa aatagtggta agaaagaaaa ataaaataga
     8641 aaaagaagat ggaaaagagc agcttgactg aaaaaggcag agctgttctt tttttgtgca
     8701 tgtgcttatt ggaatgaaag tggtttgaac caaagtatac caaaccaact tattataaga
     8761 aatctacaaa aacaaacagg tgaaattgca taaaaagccg aaacggaata aaaaatatat
     8821 cactttgttg acaagaagaa aagattgtgg tatatattgg tatataaaac agagacgcaa
     8881 attggtataa aaataaaccg aaaggagaag aggtatgaac acagcatttt taaatgcaaa
     8941 aaatgatttc ctggctgcag ccaagcgtac ttatgaaagc ggtatccaga caggaacagg
     9001 gggaaactta agtgtcagga t
//
LOCUS       DBMDUA010000112         8979 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3596_length_8979_cov_2.824397, whole genome shotgun sequence.
ACCESSION   DBMDUA010000112 DBMDUA010000000
VERSION     DBMDUA010000112.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8979)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3596_length_8979_cov_2.824397"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="ACZPCL_08970"
     CDS             complement(<1..1190)
                     /locus_tag="ACZPCL_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024910.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR1162632.1"
                     /translation="MDNKKLNFWECMSFCIGQIVGSGVFVLTGIVIGLTGHGAPYGFV
                     LAALIAVCQLVPMATLASSMPATGGSYVYAKKLIGPRASFVFLMMFVLQQVLIATFAI
                     GFASYVAVIFPDVNQKLVALGALTLAVVVNMIGLKTSAKVQKFMVALLLVSLFVYIVF
                     GLPKVDWSALALNSENIMPNGPMKFLRGAAMLSFACAGAKFLAENGGEVENPGKTIPK
                     AMVLSTLLVAIFYALVGIVAACVLPVEEVAGVNISVVAKSVFPAPVYLFFVIGGAWFA
                     LLTTLNGTLSWTTRSLQRAAMDGWLPEACAKENKGGTPVILLLFFFVVGIIPIVTGMN
                     TSDIANMGTGCGKLTDLLMVYACFRLPKVFPEEYKKSALYMSPAKLNVALAVVFVVLA
                     ATSYV"
     gene            complement(1193..2374)
                     /locus_tag="ACZPCL_08975"
     CDS             complement(1193..2374)
                     /locus_tag="ACZPCL_08975"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR1162633.1"
                     /translation="MKYDFNKVQDRIGSDSIKWEKQLKFGTKTGLLPFWIADTDFATL
                     PEAVEAMKKRLEHPVFGYTTTGERTLETVRGWYKRRHQVDLPVSAFSPSEGVVTSIWF
                     SIRGFTQPGDGVLVFTPVYDPFFAVINNQGRKQVDCPLVYSEGRYTIDWEDLEKKLAG
                     GDVKAMIFCNPHNPVGRVWTEDEVRNIVELCKKYSVWLFSDEIHGDVVLYGHQYTSAA
                     RFADVYDHMLVYTAISKTFNMAGLHSSCTIIPNPEYKKIQEDTLRQAWLMSPNCMANS
                     AIEACYTYGDEWVDEQNAYLTENAEFVTSYLKEHAPQIKPVKPEGTYLMWLDCSELSM
                     TSEEIVKILASEYKMAVGGGAGYQGNGEYFLRFNIGCPRETLEKGMEILAQFVADKKG
                     V"
     gene            complement(2673..3398)
                     /locus_tag="ACZPCL_08980"
     CDS             complement(2673..3398)
                     /locus_tag="ACZPCL_08980"
                     /inference="COORDINATES: protein motif:HMM:NF019349.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR1162634.1"
                     /translation="MNKLSNKPLYDQVFDQIKDMIRSGTYCAGDQLPSEKELMERMGV
                     SRVTVRQALRLLADAGIIETRKGKGSIVSVDWKNLLDPGELKDQAEKYWRQFELSTKA
                     RRLIEPAIAKQAAFMATPEDIARLERIYENDQVVIEGPNLYVEEGRGLQSFHGCLWEI
                     VKNPLLEPVWNAVVLPSNEIRTLPLIFPVDQELNREQVRIQHGNILEAVKNHDGEYAY
                     FHMLVHCDWIYNTYKQYFDEFCR"
     gene            complement(3786..4754)
                     /gene="dusB"
                     /locus_tag="ACZPCL_08985"
     CDS             complement(3786..4754)
                     /gene="dusB"
                     /locus_tag="ACZPCL_08985"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR1162635.1"
                     /translation="MKFKIGDTLLDNNVFLAPMAGVTDLPFRLLCREQGAGMSVTEMV
                     SAKAILYKNKNTKELLAVDRAEGPVSVQLFGSDPEIMAAIAAQIEDGPYVAIDVNMGC
                     PVPKIVNNHEGSALMKDPAQAEKVLTAMVKAVKKPVTVKFRKGFNDQNINAVEFAKMA
                     ESCGVAAVAVHGRTREQYYSGKADWDIIRQVKEAVKIPVIGNGDVFTPEDAKRLLDET
                     GCDGIMVGRGAKGNPWIFKRITHYLETGELLPGPTLMEVRDMILRHGKMLTEYKGEMI
                     AMREMRSHMAWYTKGMRNSAALRCEINQVETLEGLAELLEKRMEAE"
     gene            complement(4744..5130)
                     /locus_tag="ACZPCL_08990"
     CDS             complement(4744..5130)
                     /locus_tag="ACZPCL_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6145 family protein"
                     /protein_id="HIR1162636.1"
                     /translation="MTDKVVLCGANSYEQKYYFNEEFQSLPQSVKDELHIMCVLFTED
                     VGGILTMEYDEKGNLDFQVISEEGDYLFDEIGSVLKIKEYQETKKELLEALELYYRTF
                     FLGENVDWEEEKEDQEGDDKGETDEV"
     gene            complement(5319..6293)
                     /gene="pfkA"
                     /locus_tag="ACZPCL_08995"
     CDS             complement(5319..6293)
                     /gene="pfkA"
                     /locus_tag="ACZPCL_08995"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1162637.1"
                     /translation="MAKSVKTIGVLTSGGDAPGMNAAIRAVVRGAINKGLKVKGIMRG
                     YAGLLDEEIVDMDTTSVSDIINRGGTILYTARCMEFTKPEGQERGAEICRKHNIDGIV
                     VIGGDGSFRGAGKLSALGINTIGLPGTIDLDIACTDYTIGFDTAVNTAMEAIDKIRDT
                     STSHERCSIVEVMGRNAGYIALWCGIANGAEDILLPERYDGDEQALINRIIENRKRGK
                     KHNIIINAEGIGHSGSMAKRIEAATGIETRATILGHMQRGGTPTCKDRVYASIMGARA
                     AELLAEGKSNRLVAYKHGEFVDYDIQEALNMKKDIPEEQYEIAKLLAR"
     gene            complement(6381..>8979)
                     /locus_tag="ACZPCL_09000"
     CDS             complement(6381..>8979)
                     /locus_tag="ACZPCL_09000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR1162638.1"
                     /translation="WTYLNHLCREGFKTRYPDDDGTLSRRLDYELGVIKTMGYVDYFL
                     IVWDFINYARSQNIMVGPGRGSAAGSIVSYTLGITNIDPVRYNLLFERFLNPERVSMP
                     DIDVDFCYERRQEVIDYVVRKYGKDQVVQIVTFGTLAAKGVVRDVGRVLDLPYAMCDS
                     IAKMIPNDLGMTLDKALTANPDLKKLYNEDEQVKYLIDMSKRLEGLPRHTSMHAAGVV
                     IGSRSIDEFVPLSRAADGTITTQFTMTTIEELGLLKMDFLGLRTLTVIQNAVRLAEKD
                     YGIKLDMDHIDYNDKKVLESIGTGRTEGVFQLESGGMKGFMKELKPENLEDIIAGISL
                     YRPGPMDFIPRYLKGKNDKTSITYECPQLEPILSPTYGCIVYQEQVMQIVRDLAGYTM
                     GRSDLVRRAMSKKKTAVMEKERQNFVYGNEAEGVKGCIANGIDEKTANHIYDEMIDFA
                     KYAFNKSHAAAYAVVSYQTAYLKYYYPKEFMAALMSSVMDNVSKFSEYILTCRRMMDI
                     AILPPDINEGESGFSVSGDGIRYGLSAIKSVGRPVVDAILEERKKNGIFSSMEDFINR
                     MTNKEVNRRTIENFIKSGAMDSLPGTRRQKVAVAPILLDNKARERKNAWEGQMSLFDL
                     VSEEEKKEYQVSFPDVGEYSKEELLAFEKDILGVYISGHPLDDYEGLWRKNITATSAD
                     FIVDEETEEAVVKDGMKVVIGGLVAGKVVKTTRSNQLMAFITLEDLMGSVEVIVFPKN
                     YEADRDVLTEDSKIFIKGRVSLGDEPVGKLVCEQVIPFSKVPRQLWLQFEDKEIYQAM
                     EGEILGILKESEGPDSVVIYLKKERAKKILPANWKVEAAGELMETLICKLGEKNVKLV
                     EKNLINLGK"
BASE COUNT         2292 a         2291 c         1773 g         2623 t
ORIGIN      
        1 acataggagg tagctgccag tacaacgaat acaacagcta aagctacatt taactttgca
       61 gggctcatgt acagagcaga tttcttgtat tcctcaggaa ataccttagg cagacggaag
      121 catgcataaa ccattaacag gtcagtgagc ttaccacaac cagtacccat atttgcaata
      181 tcagaagtgt tcataccggt tacgatcggg atgataccaa ctacaaagaa gaataataac
      241 agaataacag gtgtgccgcc cttattttcc tttgcgcagg cctctggcag ccatccgtcc
      301 atagctgctc tctgcaggga acgtgtggtc caggataagg taccatttaa ggttgtcagt
      361 aatgcgaacc atgcaccacc gattacgaag aacaggtata caggtgcagg gaatacagat
      421 tttgctacta cagaaatgtt tacaccagct acttcttcaa caggaagaac gcaggcagca
      481 acgataccaa ctaatgcata gaaaattgca acaagcagtg tagataatac catagccttt
      541 ggaatggtct tgccaggatt ttctacttca ccgccgttct cagctaagaa ctttgcacca
      601 gcacatgcga aggaaagcat tgcagcgccg cgaaggaatt tcattgggcc gtttggcatg
      661 atgttttcac tgtttaatgc cagtgcagac cagtctacct taggaagacc aaatacaatg
      721 tatacaaaca gagaaacaag taataatgct accatgaact tctgtacttt tgcagaagtc
      781 ttaaggccga tcatgtttac tactactgcc agagtcagtg cgccaagtgc tactaacttc
      841 tgatttacat cagggaagat aacagctaca tagcttgcaa agccaatagc gaaggttgcg
      901 atcagtacct gctgaagaac gaacatcatt aagaatacga aagatgctct tggtccgatc
      961 agtttcttag catacacgta gctgccgccg gttgccggca tactggatgc cagagttgcc
     1021 attggaacca gctggcatac agcgatcagt gctgcaagaa cgaagccgta aggtgcgccg
     1081 tgtccggtca gtccgattac gataccggtc agtacgaata cgccggaacc aacgatctgg
     1141 ccgatgcaga aactcataca ttcccagaaa ttcagttttt tgttgtccat agttataccc
     1201 ctttcttatc tgccacaaac tgtgccagta tttccatacc tttttcaaga gtttctcttg
     1261 ggcagccaat attaaagcga aggaaatatt ctccattgcc ctgatatccg gcgcctcctc
     1321 ctactgccat cttatactca gatgccagga tctttacgat ctcctcggaa gtcatggaaa
     1381 gttcgctgca gtctaaccac atcaggtaag taccttctgg cttaacaggc ttgatctgtg
     1441 gtgcatgctc tttcagatag ctggtaacaa attcagcatt ttctgtaagg tatgcattct
     1501 gttcatctac ccattcatca ccatatgtat agcaggcttc aatggcactg tttgccatgc
     1561 agttagggct cataagccat gcctggcgaa gagtgtcttc ctggatcttc ttgtactccg
     1621 gatttggaat aatggtacag gaagaatgaa gacctgccat gttaaaggtc ttgctgatcg
     1681 cggtgtagac cagcatatga tcgtacacat cagcgaaacg tgcagcagaa gtatactgat
     1741 gtccgtaaag gactacatct ccatggatct catcagagaa cagccatacg gaatatttct
     1801 tacacagttc tacgatattt ctgacctcat cttctgtcca tacacggcct accgggttgt
     1861 gggggttgca gaagatcatg gcctttacat caccaccggc taatttcttt tccagatctt
     1921 cccagtcaat ggtgtaacgg ccttcgctgt aaaccagtgg acagtcaacc tgttttcttc
     1981 cctgattgtt gatcacagcg aaaaatgggt catatacagg agtaaacact aaaactccgt
     2041 ctcccggctg tgtaaagccg cggattgaga accagataga tgtaacaaca ccttcagaag
     2101 gagaaaaagc actgacaggc agatctacct gatgacggcg cttataccag cctcttactg
     2161 tttccaaagt tctctctcct gtcgtggtat agccgaaaac cggatgttcc agacgtttct
     2221 tcattgcctc taccgcttcc ggcaatgttg caaagtcagt atcagcgatc cagaaaggaa
     2281 gaagtcctgt ctttgtacca aatttcagct gtttttccca cttaatggag tcagagccaa
     2341 tgcggtcctg tactttgtta aaatcgtact tcattactgc acctccaaaa tgatgtaaaa
     2401 ctgaatgtgt ttttcaacac tttcattgta taatgccaaa tccccaggcg gtacgggccc
     2461 tgcactgtcc gggaaaatta cagccaaaga atatgtgata ccagggtcaa aaggtcgaaa
     2521 aaccgacgca agcttgcttg caggaggctt tttgaacttg ggacccgcca aaaacatgag
     2581 taagtagcca ttttttgaag aaaaatgagc ggattacgaa tgtttttaag gattgcgaga
     2641 gtgcatagca cgtagcaatc cggtatcaaa gtttacctgc agaattcatc aaaatactgc
     2701 ttatatgtgt tataaatcca gtcgcagtgg actaacatat gaaaatatgc gtattcacca
     2761 tcatggtttt tcactgcttc cagaatattt ccatgctgga tacgtacctg ttctctgttt
     2821 aactcctggt ctacagggaa aataagaggc agtgtacgga tctcattgga aggaaggact
     2881 accgcgttcc ataccggctc taacagggga ttttttacga tctcccataa gcagccgtgg
     2941 aaagattgaa gtcctctgcc ctcttccaca tacagattcg ggccttcgat caccacctgg
     3001 tcgttttcgt aaatacgctc cagtctggca atatcttccg gtgtagccat aaacgccgcc
     3061 tgctttgcaa tagcaggttc gatgagcctt ctggcctttg tggagagttc aaattgacgc
     3121 cagtattttt ctgcctggtc ttttaactct ccgggatcca gcaaattttt ccagtcaacg
     3181 ctgacgatac ttccctttcc tttgcgggtc tcaataatac ctgcatcagc taaaaggcga
     3241 agtgcctggc ggacagttac tctgctgact cccatacgtt ccatcagttc tttttcgctg
     3301 ggaagctgat ccccggcgca gtaggtacca ctgcgtatca tatctttgat ctggtcaaac
     3361 acctgatcat ataaaggttt atttgataat ttattcacag tgttgcctcc tctcctgtct
     3421 gcaactgtat ggtgtaataa ccatacacca tgtattaaaa taagtcaagt atattttaga
     3481 tttttttaac ttttctacaa gtttttcttt attttttagg cacatttttc taaaaacccc
     3541 ctattttttc tattttcctt tggtttattc cgccataact tgtattaatt aagacatgtg
     3601 ctgttctact ttattttcat aacatgtatt aaattaaaat aagttatatc tctaaaaaag
     3661 aaagaaagca ggtaagactt tccttctgtc ctgcctgctc tttgctgtca tttcgttata
     3721 tttttatcta aataacgctt ttttattttt acctgttcct ggttttattt ttcttttacc
     3781 agctcttatt ctgcttccat ccgcttttcc agaagttctg ccagaccttc caatgtctct
     3841 acctggttga tctcacagcg cagggctgcg gaatttcgca ttcccttggt gtaccaggcc
     3901 atgtggctgc gcatttcacg catggcgatc atctcgccct tgtactctgt gagcatcttt
     3961 ccatggcgca ggatcatatc ccgtacttcc ataagggtag gtcccggcag cagttcacct
     4021 gtttccaaat aatgggtaat gcgtttaaag atccagggat ttcccttggc tcctctgcct
     4081 accataatac catcacagcc ggtttcatct aacagtcgtt ttgcatcctc aggtgtaaat
     4141 acatccccgt ttccgatcac cggtatcttt accgcctctt ttacctggcg gataatatcc
     4201 cagtctgcct ttccggaata atactgttcc ctggtgcggc catggactgc cacagctgct
     4261 acgccgcagc tttctgccat ttttgcaaac tcaacggcat taatgttctg gtcattaaag
     4321 cctttacgga attttacagt caccggcttt ttcactgcct ttaccatagc agtaagcact
     4381 ttttctgcct gggccggatc tttcatcagg gcagaacctt cgtgattgtt gacgatcttt
     4441 ggcaccgggc atcccatatt tacatcaatg gcaacatagg gcccgtcctc gatctgggct
     4501 gcgatcgctg ccatgatctc cggatcggag ccaaacagct gtacagaaac aggaccttct
     4561 gcccggtcta ctgccagaag ttcttttgtg tttttgtttt tgtataaaat agccttggca
     4621 ctgaccattt ctgtcactga catgcctgca ccctgttccc tgcataaaag gcggaaaggc
     4681 agatctgtca cacctgccat aggtgccaga aatacgttgt tatctaaaag ggtatcaccg
     4741 atcttaaact tcatctgttt ctcccttatc gtcgccttcc tggtcctctt tttcctcttc
     4801 ccagtctaca ttttccccta aaaagaaggt tctgtaataa agctccaacg cttccagaag
     4861 ctctttcttt gtctcctggt actccttgat ctttaataca cttccgatct catcaaagag
     4921 ataatctccc tcttcagaga tgacctgaaa atccagattt cccttttcat cgtattccat
     4981 agtaaggata ccgcctacat cttctgtaaa aagaacgcac ataatgtgca gttcatcttt
     5041 tacagactga ggcagggact ggaattcctc gttgaaatag tatttctgct cataggaatt
     5101 ggctccgcag agcactactt tatctgtcat attttctttc ctttctatac atcaccccac
     5161 cacactcaca aacccgcact gacgtgctct actgcctgtt tacgcaggcg gtttgttcgt
     5221 taatggctta tgaaacacat gaaagtccgc tgcaccggca gatgccggta acggcggact
     5281 ttgtattctt atcatacagc tggcagacca gacaagtatt atcttgccag cagctttgcg
     5341 atctcatact gttcttcagg aatatccttc ttcatattca gtgcttcctg aatatcgtaa
     5401 tctacaaact caccatgctt gtaagctacc agacggttgc tctttccttc tgctaacagt
     5461 tctgccgctc ttgcacccat aatagatgcg tatacacggt ccttacaggt aggtgtaccg
     5521 ccacgctgca tgtggcctaa aatggtagca cgggtctcga taccggttgc tgcttcaata
     5581 cgctttgcca tggaaccgga atgtccgatg ccctctgcat tgatgatgat gttatgtttc
     5641 ttacctctct tacggttttc gataatacgg ttgataagag cctgctcgtc gccgtcatag
     5701 cgctccggaa gaagaatatc ctcggccccg tttgcaatac cgcaccataa cgcgatataa
     5761 cctgcattac ggcccataac ctcaacgata ctgcagcgtt cgtgggaggt agatgtatca
     5821 cggatcttat caatggcttc catagctgtg tttacagctg tatcaaagcc gatggtataa
     5881 tccgtgcatg caatatccag gtcaatggtt ccaggaagtc cgatggtgtt gatgcccagt
     5941 gcagaaagtt ttcctgcacc acggaaagat ccgtcaccgc cgatgactac gataccgtca
     6001 atgttatgct tgcggcagat ctctgcgcct ctctcctgtc cttccggttt tgtaaattcc
     6061 atacatcttg ctgtataaag aatggtaccg ccccggttaa taatatcaga tacactggta
     6121 gtatccatat ccacgatctc ttcatccaaa agtccggcat atcctctcat aatacctttt
     6181 actttcaggc ccttattgat cgcgcctctt acaaccgcac gaatggccgc gttcattcca
     6241 ggagcatcac ctccgctggt taatactcca atggtcttta ctgactttgc cataccaatc
     6301 tacctccttg ttttaagcac atctccaggt ctgatgtgcg ttgcttctgt tcttaaataa
     6361 atacacaacg ctattctaat tcattttccc agattaatca agtttttttc tacaagcttg
     6421 acattttttt caccaagttt acaaatcaat gtttccatca gctcccctgc agcctctact
     6481 ttccagtttg caggcagtat tttctttgcc cgctccttct ttaaatagat aactacgctg
     6541 tccgggcctt cactttcttt taaaatgcct aaaatttccc cttccatggc ctgatatatt
     6601 tctttatctt caaactgcag ccacagctgt cttggaacct ttgaaaatgg gatcacctgc
     6661 tcgcatacca gtttgcccac cggttcatct cctaaggaca ctctgccctt gataaagatc
     6721 ttggagtctt ctgttaaaac atcccggtcc gcttcgtaat ttttcggaaa aacgatcact
     6781 tccacagagc ccatcagatc ttccagtgtg ataaatgcca tcagctggtt gctccttgtg
     6841 gtctttacca cttttccggc caccagaccg cctattacca ccttcatgcc atcttttacc
     6901 actgcttctt ctgtctcttc atctacaata aaatcagcag atgtggcagt gatattcttt
     6961 ctccagagac cttcataatc atctaatgga tgaccgctga tgtatacgcc cagaatatcc
     7021 ttttcaaaag ccagcagctc ttccttggaa tactctccca catccgggaa agatacctga
     7081 tattcctttt tctcttcctc tgataccagg tcaaacagac tcatctggcc ttcccaggcg
     7141 tttttacgct cccttgcttt attgtcaaga aggatcggcg ccactgccac cttctgccgt
     7201 ctggtgccag gaagagagtc catagctccg gacttaataa agttttcaat ggtacggcgg
     7261 ttcacctctt tgttggtcat acggttgata aaatcttcca tggaactgaa aataccgttt
     7321 ttcttccgtt cttccaggat tgcgtctacg accggccttc ccacgctttt gatcgcagaa
     7381 agtccatagc ggataccatc tcctgataca gagaagccgc tttctccttc gttgatatcc
     7441 ggcggcagga tggcaatatc catcatacgg cggcaggtta agatatattc cgaaaatttg
     7501 cttacattat ccataacaga agacataagt gctgccataa attcttttgg ataataatat
     7561 tttaaataag ccgtctgata agaaaccaca gcataagctg cagcatgaga cttattaaat
     7621 gcatattttg caaaatcgat catctcatcg taaatatggt ttgccgtctt ttcatcaatc
     7681 ccattggcga tacagccctt tactccctct gcctcatttc cataaacgaa gttctgacgc
     7741 tctttttcca tgacagccgt ctttttctta ctcatggcac ggcgcaccag atcgcttcgg
     7801 cccatggtat agccagccag gtcacggacg atctgcataa cctgctcctg gtaaacgata
     7861 cagccatagg tcgggcttaa gatgggctcc agctgagggc actcataggt aatagaggtc
     7921 ttatcatttt taccctttaa atagcgtggg ataaaatcca ttgggcccgg acggtacagg
     7981 gagataccgg caatgatatc ttccaggttc tccggcttca attccttcat gaaccctttc
     8041 ataccgccgc tttcaagctg gaacacgcct tctgtacggc ctgtaccaat agactccagt
     8101 acctttttat cattgtaatc aatatgatcc atatccagct ttatgccgta gtctttttct
     8161 gccagacgca ctgcattctg gatcaccgtc agtgtacgaa ggcccaggaa gtccattttc
     8221 aacaatccca gctcttcaat ggtcgtcatg gtaaactggg tggtaatagt tccgtctgct
     8281 gccctggaaa gaggcacaaa ctcatcaata gaacggctgc cgatcacaac gcctgccgca
     8341 tgcatggagg tatgtcttgg cagtccttcc agacgttttg acatatcgat aagatatttt
     8401 acctgctcat cctcattata aagcttcttt agatccggat tggccgtaag agccttatcc
     8461 aaggtcattc ccaggtcatt ggggatcatt ttcgcaatgg agtcgcacat ggcataaggc
     8521 agatccagca cacggcctac gtcacggact acaccttttg ctgccagtgt accaaaagtg
     8581 acgatctgta ccacctggtc cttgccgtat ttgcgcacaa cgtagtcaat gacttcctgt
     8641 ctgcgctcgt agcagaaatc cacgtcaata tcaggcatgg acactcgctc cggatttaag
     8701 aaacgctcaa agagcaggtt ataacgcacc gggtcaatat tggtaatgcc aagcgtataa
     8761 gaaacgatac ttcctgccgc agaaccacgc cctggtccca ccataatatt ctgggaacgg
     8821 gcataattga taaaatccca tacgatcagg aaataatcca catagcccat ggttttgata
     8881 acacctaatt cataatccag acggcggctt aacgtgccgt catcatccgg ataacgggtc
     8941 ttaaagccct cccggcataa gtggttcaaa taagtccag
//
LOCUS       DBMDUA010000113         8933 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3608_length_8933_cov_2.772305, whole genome shotgun sequence.
ACCESSION   DBMDUA010000113 DBMDUA010000000
VERSION     DBMDUA010000113.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8933)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8933)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8933
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3608_length_8933_cov_2.772305"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            299..1396
                     /gene="ychF"
                     /locus_tag="ACZPCL_09005"
     CDS             299..1396
                     /gene="ychF"
                     /locus_tag="ACZPCL_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR1162639.1"
                     /translation="MKLGIVGLPNVGKSTLFNSLTKAGAESANYPFCTIDPNVGVVPV
                     PDERLNKLTEMYNSEKTTPAVIEFVDIAGLVKGASKGEGLGNQFLSNIREVDAIVHVV
                     RCFDDPNVIHVDGSVDPVRDIETINLELIFSDIEILERRIAKQSKGAFNNKDLAKEVE
                     LLKAIKAHLEDGKLARSFEVEDEDDREFINSLNLLTWKPVIFAANVAEGDLADDGASN
                     HYVQEVREYAKANDCEVFVICAEIEQEIAELDDDEKQMFLDDLGLKESGLEKLIAASY
                     RILGLMSYLTAGPQESRAWTIKVGTKAPQAAGKIHSDFERGFIRAEVVSYDDLMACGS
                     MNAAKEKGLVRSEGKEYVMKDGDVVLFRFNV"
     gene            1512..2396
                     /gene="lgt"
                     /locus_tag="ACZPCL_09010"
     CDS             1512..2396
                     /gene="lgt"
                     /locus_tag="ACZPCL_09010"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR1162640.1"
                     /translation="MELLTSTGADLSFVHLGITIEHLRNSISIFGFRIAFYGIIIGCG
                     MLAGMAVAFSDAKRRGQDPDLYMDFALYGIIFSIIGARLYYVIFEWDNYKNDLLQIFN
                     LRAGGLAIYGGVIGAVLTLLIFTKVRKQSFFSMADSGVLGLITGQIIGRWGNFFNCEA
                     FGGYTDNLFAMRIKESIVNPSMISAGLLEHEIVENGVKYIQVHPTFLYESCWNLCVLA
                     FMLWYRKRKKFDGEMLWVYFLGYGLGRVWIEGLRTDQLKLPGTGLAVSQLLSAALVGA
                     AVGVLHYKHSKLKKQKNI"
     gene            2635..3060
                     /gene="mraZ"
                     /locus_tag="ACZPCL_09015"
     CDS             2635..3060
                     /gene="mraZ"
                     /locus_tag="ACZPCL_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR1162641.1"
                     /translation="MFMGEYNHTVDAKGRLIVPSKFREQLGDEFVITKGLDNCLFVYD
                     NSEWAALEEKLRALPITNTAGRKFSRFLLGSAATCEVDKQGRILLPSVLREFAGIGKD
                     AVLVGVGSRIEIWNKDKWMEANTFDDMEEIAEHMEGLGI"
     gene            3060..4013
                     /gene="rsmH"
                     /locus_tag="ACZPCL_09020"
     CDS             3060..4013
                     /gene="rsmH"
                     /locus_tag="ACZPCL_09020"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR1162642.1"
                     /translation="MEEHGFVHKSVLLYETVGSLNIKPGGIYVDGTLGGGGHAYEVCK
                     RLGGGRLIGIDQDADAIRAAGERLAPFKDKVTIVRSNYQNIENVLKELNIEKVDGIYL
                     DLGVSSYQLDTASRGFTYREDAPLDMRMDQRNPTTAADIVNGYSEMELFHIIRDYGED
                     NFAKNIAKHIVKARAEKPIETTGELSEIIKAAIPAKVRATGGHPAKRTFQAIRIELNH
                     ELDVLENSIDTMIRLLNPEGRLSIITFHSLEDRIVKTRFRTNENPCICPPDFPVCVCG
                     RKSMGKVITRKPILPSKEELEENSRSKSAKLRVFERAGKEE"
     gene            4018..4482
                     /locus_tag="ACZPCL_09025"
     CDS             4018..4482
                     /locus_tag="ACZPCL_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HIR1162643.1"
                     /translation="MASYIHGTGAEKLAPSEREERLQRKRRQDVGRSVKRQHRIRRNQ
                     ERAMYMDLPYVIMLTLASICTLYLCVNYLHLQSAITARMHNIEAMEEKLEKMRTENDA
                     LETSINTSIDLNEIYEIATKELGMVYAKKDQVLLYDKTESEYVRQYEDIPEH"
     gene            4460..6811
                     /locus_tag="ACZPCL_09030"
     CDS             4460..6811
                     /locus_tag="ACZPCL_09030"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1162644.1"
                     /translation="MKTSRNTKNGAHNKKENLFRRRTFVKYMQEKLAITIILISLALF
                     ALIFVLYRIVNENSERYNKIVLSQRQQEYASRTIPYRRGDIVDRNGTYLATSEKVYNL
                     ILDPSQIMDKPDYYLEPTIQALVDVFGFDGNELRGLIQERPKQAYLRYKNGRQLSYDQ
                     KTEFEQTEKERNAAYRKSDDDNQSRFRVTGVWFEDEYRRIYPYGSTACNVIGFASGDG
                     SNGTGGIEQYYNDSLIGVNGREYGYLDEDANLEGVVKSAVNGRTVVSTIDVNVQNILQ
                     KYIDEWQTSVGSHVTAAIAMNPNNGEILGMATSNTFDLNNPRNTDGYTEDELLALGKK
                     EAVGVYRRENPDQTITEDQVLDHYSREEVISYGKQVAWNQLWRNFCVSDTFEPGSPSK
                     ILTVAAGLEEGILKGNEYFDCGGYLHVGDWDIKCTAYRRGGHGSLSLTESIMQSCNVA
                     MMRIGAMMGRDIFTKYQTIFGMGQKTGVDLPGEADAAGLVYSADKMGPTELATNAFGQ
                     NYNCTMIQMAAALCSVINGGSYYEPHVVRQILNEQGSVVERKDPVLVRETVSQSTSDF
                     LKEAMFQTVENGTGGAAKVAGYQVGGKTGTAEKQPRSAKNYLVSFAGFAPADDPQLFV
                     YVVVDVPGFPPGDQQSHSSFASNIFSKIMSEALPYLNVFPTGDLPEESQDANGQTPSE
                     GINTPAPSETGSEENGESQPEESQKVYETDEYVDTDGGSGIPDAVPGDPNAPELPAAD
                     PGGDTEAGAQLPSANESSSEAGNSQEGSSENSTEEETSQSQSE"
     gene            6882..8648
                     /locus_tag="ACZPCL_09035"
     CDS             6882..8648
                     /locus_tag="ACZPCL_09035"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1162645.1"
                     /translation="MDHSGGNLTKHRKNIAVLWLLIAIAVLGLLGRLVFLMVFRSEHY
                     SQMALELHQRERSIKAARGRILDAKGKVIADNRTVCTVSVIYNQVKDREKVTDELSEI
                     LGIDRETVQKKVEKRSSREIIKTNVDKETGDAVRKLELPGVKVDEDYKRYYPYGSLAS
                     KVLGFTGGDNQGVIGLEVQYDAYLKGTNGKILTMTDARGVEMENGAEDRIEPVAGNDL
                     RISLDMEIQQYAEQLAYQTLEKKNAKKVSIIVMNPQNGEIYAMVNVPEFDLNDPFTPD
                     QNLRSESLRNQAAERDKTVKQQELLNAMWRNTCINDTYEPGSTFKIITAAAGLEEGVV
                     KLTDPFSCPGFRVVEDRRIRCHKAGGHGAETFLQGVMNSCNPVFIDVGQRLGSDNYYK
                     YFVQFGLKQKTGIDLPGEAATIMHKKENIGPVELATISFGQSFQITPIQLITTAASIV
                     NGGRRITPHFALGTVSPDGKNYREFSWPLGKRILSEETSATMRYVLEQVVAEGSGRKA
                     AVEGFSIGGKTATSEKLPRSRKKYISSFLGFAPANDPKVIALITIDEPEGIYYGGTIA
                     APVIGTLFENILPCMDTSKPLS"
     gene            8714..>8933
                     /locus_tag="ACZPCL_09040"
     CDS             8714..>8933
                     /locus_tag="ACZPCL_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR1162646.1"
                     /translation="MIRETILAIIIAFAVSALLCPVIIPFLHKLKFGQQVRDDGPQSH
                     LKKQGTPTMGGLIILTSIIITSVFYIPSY"
BASE COUNT         2728 a         1740 c         2291 g         2174 t
ORIGIN      
        1 acagccattg ggaaaactgt tattcccata ttttgaaagt gctgccctcc cacatcatag
       61 ccggaagtgt tggtgtggta atgcgaaaag gagaagaaaa aagactggaa gacctggtaa
      121 atgagatcca aaaagcagtc tgaaagcgtg ttgaaccttg aaaaagagaa atccttatgc
      181 tataatcaat gattgcatta gactcttaaa gaaatggtac ataattggta cataaagaat
      241 acaaagaaag gcgaaaacct ttgatctatc cgggggtttt tgagcaggta aagcaaaaat
      301 gaaattaggt atcgtaggac ttccaaacgt aggaaaaagt acacttttta actctctgac
      361 caaggctggt gcagagtctg caaactatcc gttctgtacg atcgacccaa acgtaggtgt
      421 tgtaccggtt ccagatgagc gtttaaataa gctgacagag atgtataact ctgaaaagac
      481 tactcctgca gtgatcgagt tcgtagatat cgcaggtctg gtaaagggag cttccaaggg
      541 tgaaggtctg ggcaaccagt ttttatccaa tatccgtgaa gtagatgcga ttgttcacgt
      601 tgtacgttgc ttcgatgatc ctaatgtcat ccatgtagac ggaagcgtag atcctgtaag
      661 agatattgag acgatcaact tagagctgat cttctctgat attgagatcc tggagcgtcg
      721 tattgcaaag cagtccaagg gtgctttcaa taataaggat cttgcaaaag aagtagaact
      781 gttaaaggca atcaaggcac atctggaaga cggcaaactg gcacgcagct ttgaagtaga
      841 agacgaggat gacagagaat ttatcaattc cctgaacctg ctgacctgga aacctgttat
      901 ctttgctgca aacgtagcag aaggtgacct ggcagatgat ggcgcttcca atcactatgt
      961 acaggaagta cgggaatatg caaaggcaaa tgactgcgaa gtattcgtga tctgtgctga
     1021 gatcgaacag gagatcgcag agttagatga tgatgagaag cagatgttct tagatgactt
     1081 aggcttaaaa gagtctggtc ttgagaaact gatcgcagca agttaccgta ttttaggact
     1141 gatgagctat ttaacagcag gtcctcagga gtccagagca tggaccatca aagtgggaac
     1201 caaggctcct caggctgcag gaaagatcca cagcgatttc gagcgtggtt tcatccgtgc
     1261 agaagtagta agctatgatg acctgatggc atgcggcagc atgaacgcag caaaggaaaa
     1321 aggtctggta cgttccgaag gtaaggaata tgtgatgaag gacggagatg ttgtactgtt
     1381 ccgtttcaat gtataatgat tgagaattaa tcgatagtaa aaaacaaact gcctgctctg
     1441 caggcacttg ttttatatgc agatagccat ttaacagggc agctgcaaat aacggaaagg
     1501 aacagtatat tatggaatta ctcacatcca caggtgcgga cttaagtttc gtccatcttg
     1561 ggatcaccat agaacacctg cgaaacagca tcagtatctt tggctttcgc attgcatttt
     1621 atggtattat cattggctgc ggtatgttag ccggtatggc ggtggctttc tctgatgcca
     1681 aaaggcgggg acaggacccg gatctgtata tggattttgc actgtatggg atcatttttt
     1741 ccattatcgg tgccagactc tactatgtga tctttgagtg ggataattat aaaaatgatc
     1801 tgctgcagat ctttaacctt cgcgccggcg gactggctat ttacggcggt gtgatcggag
     1861 cagtactgac ccttcttatt tttacaaaag tacggaaaca gtccttcttt tccatggcag
     1921 acagcggcgt attaggactg atcacaggac agatcattgg ccgctggggc aacttcttta
     1981 actgcgaggc atttggcgga tacaccgata acctgtttgc catgcgcatt aaagaaagta
     2041 ttgtaaaccc gtctatgatc tcagccggac tgttagaaca tgagatcgtg gaaaatggtg
     2101 taaaatacat ccaggtacac cccacctttc tttatgagtc ctgctggaac ctgtgcgtat
     2161 tggcatttat gctctggtat aggaagagaa agaaatttga cggtgaaatg ctgtgggttt
     2221 atttcctggg atatggtctg ggaagagtgt ggatcgaagg cctgcgtacg gatcagctga
     2281 agcttccggg aacaggactg gcagtttccc agctgctttc cgcagcttta gtgggagcag
     2341 cagtaggtgt cctccactac aaacacagta aattaaaaaa gcaaaaaaat atctaaaaat
     2401 atctaaagaa tttcaaagct gcagaagacg atttctgcag cttttttgtt gttattttga
     2461 aagaaaaacg taaaaaatcc ttgtcatttc cctctttctg tactattata aacatacaag
     2521 tggtagaaag tggtgcaaag tggtagagga tggttgttgc gtggatgaag tggaggtata
     2581 aggagcctct tctgcatccc gccttgccat gtaatgtaca agcaggtgat agctatgttc
     2641 atgggtgaat acaatcatac agtggatgcg aaggggcgcc tgatcgttcc ttccaagttc
     2701 agagagcagt taggggacga attcgtgatc acgaagggac tggataactg cctttttgtg
     2761 tatgacaatt ccgaatgggc tgcattagaa gaaaagcttc gtgcattacc gattaccaac
     2821 accgcaggac gtaagttttc acgtttcctg ttgggaagcg ccgccacctg tgaagtagac
     2881 aagcagggcc gtatccttct cccctctgtt ttaagagaat ttgcagggat cgggaaagac
     2941 gcagtgcttg taggcgttgg aagccgcatt gaaatctgga ataaggataa atggatggaa
     3001 gccaacacct ttgatgatat ggaagaaatt gcagaacata tggaaggctt gggtatttga
     3061 tggaggaaca tggatttgta cataagtctg tactgctgta tgaaacagtg ggcagcctga
     3121 atataaagcc gggtggcatt tacgtagacg gcaccttagg cggcggcgga catgcctatg
     3181 aggtatgtaa gcggttgggc ggcggaaggc tcattggaat tgaccaggat gcagatgcta
     3241 tccgtgcggc gggagagaga ctggctcctt ttaaagataa ggtgaccata gtcagaagca
     3301 attatcagaa tattgaaaat gtactgaaag agttaaatat agaaaaagta gacggtattt
     3361 atctggatct gggagtgtcc tcctatcagt tagatacagc gtccagagga tttacctaca
     3421 gggaagatgc tcctcttgat atgcgtatgg atcagagaaa tcctaccaca gcagctgata
     3481 ttgtaaatgg atacagtgaa atggaactgt tccacattat cagggattac ggggaagaca
     3541 attttgcaaa aaacattgca aagcacattg taaaggcgag agcagaaaag cccatcgaga
     3601 ccaccggtga actgtctgag atcataaaag cagccatccc ggcaaaagta cgtgctacag
     3661 gaggacatcc tgcaaaacgt accttccagg ctatccgtat tgaattaaat catgagctgg
     3721 atgtactgga aaattcaatc gatacaatga tccgtctttt aaatccggag ggaagattaa
     3781 gtatcatcac cttccactcc ttggaggaca ggatcgtaaa aacaagattt cgtaccaatg
     3841 aaaatccatg catctgtcca ccggatttcc cggtgtgcgt atgcggaagg aagagtatgg
     3901 gcaaagtgat cacacgaaag ccgatccttc catcaaaaga agaactggag gagaacagcc
     3961 gttcaaagag cgcaaaactt cgggtatttg agcgtgcggg aaaggaggaa taaggttatg
     4021 gcttcttaca ttcatggaac cggagcagaa aagctggcgc cttccgaaag agaagaaaga
     4081 ctgcaaagaa agagaaggca ggatgttgga agaagtgtaa aacggcagca caggatccgc
     4141 agaaatcagg aacgtgccat gtacatggac ctgccatatg ttatcatgct gaccctggcg
     4201 tcgatctgta ccttatacct ttgcgttaat tatcttcatc ttcagtctgc catcacagcc
     4261 agaatgcaca acatcgaggc gatggaagaa aagctggaaa aaatgcgtac agaaaatgac
     4321 gcattggaaa caagcatcaa taccagcatt gatttaaacg agatctatga gatcgcgacc
     4381 aaggaactgg gtatggttta cgcaaaaaag gaccaggttc ttttatatga caagacggaa
     4441 agtgagtacg taaggcaata tgaagacatc ccggaacact aaaaacggtg ctcataataa
     4501 aaaagaaaat ttatttcgac gaaggacatt cgttaaatac atgcaggaaa agctggcgat
     4561 cacgatcata ttgatctcgc tggctttgtt tgctcttatt tttgttttat acaggatcgt
     4621 aaatgaaaac agcgagcggt acaacaagat cgttctgtcc cagagacagc aggagtatgc
     4681 aagcaggact attccttaca ggcgcggtga catcgttgac agaaacggca cctatctggc
     4741 aaccagtgaa aaggtataca atctgatcct ggacccaagc cagatcatgg ataaaccgga
     4801 ttattattta gagcctacca tccaggcatt agtagatgtg tttggctttg atgggaatga
     4861 actgcgcggg ctgatccagg aacgtccaaa acaggcgtat ctgcgctata aaaatggccg
     4921 tcagctttct tatgaccaga aaacggaatt tgaacagact gagaaagaga gaaatgctgc
     4981 ttacagaaaa agcgatgatg acaatcagtc aagatttcgc gttaccggcg tatggtttga
     5041 ggatgaatac cgaaggatct atccatatgg ttccactgcc tgcaatgtga tcggttttgc
     5101 aagcggggac ggcagcaatg gtaccggcgg cattgagcag tattataatg acagcctgat
     5161 cggtgtaaac ggaagagagt atggctatct ggatgaagat gccaatctgg aaggtgtggt
     5221 aaagtctgca gtaaatggac ggaccgttgt atccaccatt gacgtaaatg tacagaatat
     5281 ccttcagaaa tacattgatg aatggcagac cagtgtggga agccatgtaa cggcagccat
     5341 tgccatgaac cctaataacg gtgagatcct gggaatggca accagcaata catttgactt
     5401 aaataatcca agaaatacag acggatatac agaagatgaa ctgctggctc ttggaaagaa
     5461 agaggcagtt ggtgtatacc gccgtgaaaa tccggaccag accattacag aagatcaggt
     5521 tttagaccac tattccagag aagaagttat ctcctatggt aaacaggttg cctggaacca
     5581 gctgtggcga aacttctgtg tcagcgatac ttttgagcca ggttccccat ccaagatcct
     5641 gacagttgct gcgggacttg aagaagggat tttaaaggga aatgagtact ttgactgcgg
     5701 cggatatctt cacgtaggtg actgggacat taagtgtacg gcctacagaa gaggtggtca
     5761 cggttcctta agcctgacag agtccattat gcagtcctgt aacgtggcta tgatgcggat
     5821 cggtgctatg atgggccgtg atatttttac caaatatcag actatctttg gtatgggaca
     5881 gaaaacagga gtagacctgc ctggtgaagc agatgcagca ggactggttt attctgctga
     5941 caaaatgggc cctacagaac ttgctaccaa tgcgttcgga cagaactaca actgtaccat
     6001 gatccagatg gcagcagctc tttgttctgt gatcaacggc ggctcctact atgagcctca
     6061 tgtagtacgc cagatcttaa atgagcaggg gtctgtagta gagcgaaaag atcctgttct
     6121 ggtacgtgaa actgtatccc agtctacctc tgatttctta aaagaagcca tgttccagac
     6181 agtagaaaat ggtaccggcg gcgcagcaaa ggtagctggc tatcaggtag gcggtaagac
     6241 aggtacagca gagaagcagc cacgttccgc aaagaactat ctggtatctt tcgcaggttt
     6301 tgcacctgca gatgatcctc agctgtttgt atatgtagta gtggacgtac ctggtttccc
     6361 gccaggcgac cagcagtcac atagctcctt tgcaagcaat atcttctcaa agatcatgtc
     6421 agaagccctg ccttatctga atgtatttcc aactggagat cttccggaag agagccagga
     6481 tgcaaatgga cagacaccat ctgagggaat taatacacca gctccatctg aaacaggttc
     6541 agaagaaaac ggagagtccc agccggagga aagccagaag gtatatgaaa ccgatgaata
     6601 tgtagataca gatggcggaa gcggtatacc ggatgcagtg ccgggagatc ctaatgcgcc
     6661 ggaactgccg gcggctgatc cgggtggtga tacagaggcc ggggcccagc tgccttcagc
     6721 aaatgaaagc agcagtgagg caggaaactc tcaggaagga tcttcggaaa acagtacaga
     6781 agaagaaact tcccagtcac aaagtgaata aaggttccaa aatgaataaa aagagaaatt
     6841 acaccgtata tatttaggaa aacagcagga aagaggctgt tatggatcat agcggtggaa
     6901 atttaacgaa acacagaaaa aatatagccg tcctgtggct tctcattgcg atcgcagtgt
     6961 tggggctttt gggacggctt gtttttctta tggtcttccg gtcggagcat tacagccaga
     7021 tggcccttga actgcatcag agggaacggt ccattaaggc ggccagagga aggatcttag
     7081 acgcaaaagg aaaggtgatc gctgataacc ggacagtatg tactgtttct gtgatctata
     7141 accaggtaaa agacagggaa aaagtcacag atgaattaag tgaaattctt gggatcgacc
     7201 gggaaacagt gcaaaaaaag gtggagaaac gaagttccag ggagatcatt aaaaccaatg
     7261 tagataagga aacaggggat gcagtgcgta aattggaact gcctggcgta aaggtggatg
     7321 aagattacaa acggtattat ccctatggaa gtcttgcttc taaagtttta ggtttcaccg
     7381 ggggcgataa ccagggagtg atcgggctgg aagtacagta tgatgcctat ttaaaaggaa
     7441 ccaatggaaa gatccttaca atgacagatg ccagaggtgt ggaaatggaa aacggggcag
     7501 aggaccggat cgaacctgtg gctggaaatg atctaaggat cagtctggat atggaaatcc
     7561 agcagtatgc agagcagctg gcataccaga cattagagaa gaaaaacgcg aaaaaagtat
     7621 ccatcatcgt gatgaacccg caaaatgggg agatctatgc catggtcaat gtgccggagt
     7681 ttgatctgaa tgatcctttt accccggacc agaacctgcg aagcgagagc ctgcgcaatc
     7741 aggcggcaga aagggataaa actgtaaaac agcaggagct tttaaatgcc atgtggcgca
     7801 atacctgtat caatgatacc tatgaaccag gttctacttt taagatcata acagcggctg
     7861 caggactgga agaaggagta gttaagctga cggatccatt cagctgtcct ggtttcaggg
     7921 tagtggaaga tcggcggatc cgttgtcata aagcaggagg acatggagca gaaacttttt
     7981 tacagggagt catgaattcc tgtaatcccg tatttattga tgtgggacag agacttggca
     8041 gtgataatta ttataaatat tttgtgcagt tcgggttaaa acaaaaaaca ggcattgatc
     8101 ttccgggaga ggcagctacg atcatgcaca agaaagaaaa tataggccct gtagaactgg
     8161 cgactatttc ctttggacag tcttttcaga tcacccccat ccagctgatc acgacagccg
     8221 cctctatcgt caatggagga agacggatca cgcctcattt cgctctgggg acagtcagtc
     8281 ctgatgggaa aaattaccgg gaattttcct ggccattggg aaaaaggatc ctgtcagaag
     8341 agaccagtgc taccatgcgc tatgtgttag agcaggtagt agcagaagga agtgggagaa
     8401 aggcagcagt ggaaggattt tccattggcg ggaagaccgc aacttccgaa aaactgccaa
     8461 gaagcaggaa aaaatatatt tcttcttttt taggttttgc ccctgcaaat gatccaaaag
     8521 tgatcgctct gatcactatt gatgagccgg aagggatcta ttatggaggt accatagcag
     8581 ctccggtgat cggaacactt tttgaaaata ttcttccctg catggatact tccaaacctt
     8641 taagttgatt attctgcaaa aaagacgtat actatttacc aaataagaca tacatatgag
     8701 attgaggtgc tttatgattc gtgaaacaat attagctatt attatcgcat ttgctgtcag
     8761 cgcgctgctg tgcccggtta tcataccgtt tcttcacaaa ctgaaatttg gccagcaggt
     8821 aagagatgac ggtccacaga gccatttaaa gaagcaggga actcctacaa tgggtggcct
     8881 gatcatcctg accagcatta tcattacatc tgttttttac atcccgtcat atc
//
LOCUS       DBMDUA010000114         8869 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3631_length_8869_cov_2.466013, whole genome shotgun sequence.
ACCESSION   DBMDUA010000114 DBMDUA010000000
VERSION     DBMDUA010000114.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8869)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3631_length_8869_cov_2.466013"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="ACZPCL_09045"
     CDS             complement(<1..219)
                     /locus_tag="ACZPCL_09045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1162647.1"
                     /translation="MDLSSIFRFSDMVEYFPKILSRFPVTLLIVVVSVAGGLVLGFIL
                     AAARIFKIPVLRELAALYISFVRGTPVLV"
     gene            complement(287..1153)
                     /locus_tag="ACZPCL_09050"
     CDS             complement(287..1153)
                     /locus_tag="ACZPCL_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012707.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1162648.1"
                     /translation="MRGKWKSGIRIFAGAVLAAAVLAGCSSKTAESGAAQDAGQNTTQ
                     GNSDSQEVRKVYVAVGATFEPYTYIDENNQPAGYDVAVLQEIDNRLPQYEFQIENMEL
                     ANISVALDAKKVSIGSQQFEKNPEREKKYLFTNEGFAAYNKRLVYKKGRDDIHSIDDL
                     VGKKVSAGQGSNTAAILETYNAEHDNKIDIVYSNGATNESIYDDVMNGRLDAIVATKK
                     TFKKYNEAFGGGYDINEDSYFSESQAYFMLAQGEEQLRDDMDTALKSMKEDGTLTKLS
                     IEYTGSDYNSEE"
     gene            complement(1223..1990)
                     /locus_tag="ACZPCL_09055"
     CDS             complement(1223..1990)
                     /locus_tag="ACZPCL_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HIR1162649.1"
                     /translation="MSDKKEKDSKVKSSKAKKNVEKTQEVSVSAEKKIFPHSCTDCGV
                     LNCSKRNASYPEFCLTEKLTEKELEKVVKLYTENEENNKIAIASAEVEGGFYGKYTRV
                     EEIMEVAKRIGAKKIGIATCVGLIEESRIFARILKLNGFDVYGVACKVGSVDKTDIGL
                     DPKYTCKTGPVMCNPILQAKLLNKAGTQLNVVVGLCVGHDSLFYKYSKALATTLVTKD
                     RVLAHNPVGALYQTRAYYKRLLQQPLWDDEDDKKEEK"
     assembly_gap    2190..2199
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2200..3748
                     /locus_tag="ACZPCL_09060"
     CDS             <2200..3748
                     /locus_tag="ACZPCL_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024778.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urease accessory protein UreH domain-containing
                     protein"
                     /protein_id="HIR1162650.1"
                     /translation="MFMKILRKTLHIGGMSCVNCEHRIRYVLKTAPGIQDATVSYNTG
                     LAHVTYDTDTISLKQIREIIEKNGYTTLPETGTSDYDMKKNFVSLAVILFLYALLQHF
                     GILNLLAPGQLADTKMGYGMLFVIGLITSVHCIAMCGGINLSQCIPQPSNASDVSGSS
                     RALDIFLPALLYNTGRVISYTAIGFLLGLVGMLLGAGTQTGISYFLQGSLKMIAGIFM
                     VIMGINMLGIFPWLRPLTLRIPRFLSTKVLKKRASSRQPLFVGLLNGLMPCGPLQSMQ
                     IVALACQNPFSGALSMFLFSLGTVPLMLGLGSLVSVLGKRFAKKMTTVGAILVVVLGL
                     AMFSQGTALAGISEGPENSQNTANPQNTGTSEDQGTSTDTTDSSNAADKTPQDENANN
                     IQVIYSTLQRGRYPDIKVQTGVPIKWIINAPKGSLNGCNYRMLLRSFGIEHTLSEGEN
                     VIEFTPSKSGTFQYTCWMGMIRGNITVTDTLETSPTDPDTSTGAEETDPETEPFFNGS
                     GMGCCGS"
     gene            3814..4518
                     /locus_tag="ACZPCL_09065"
     CDS             3814..4518
                     /locus_tag="ACZPCL_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1162651.1"
                     /translation="MMEQKTILIVDDENMMREAVASYLEKQGYHVLQAETGTQALSLL
                     EKETVSFVILDLMLPDISGEEICSRIRRQSRIPIIMLTAKTMEDDMLNGLNLGADDYI
                     TKPFSLKNLYARIQAVWRRSDHDLKPLAEKFSWNAGDLVIDYNRKEVLKKGETISLTP
                     IEWKILSAFTRYPQKVFTRDELIASAFDTDFSGYDRVIDTHIKNLRKKIEDDPKNPIY
                     LCTVHGIGYKFGGNIQ"
     gene            4515..5924
                     /locus_tag="ACZPCL_09070"
     CDS             4515..5924
                     /locus_tag="ACZPCL_09070"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1162652.1"
                     /translation="MKQKKHKISTQLSAGFAVIVLITISVISLTANLLISHQFEKYVA
                     RQRETFAEQLITTLLSQYNSSNQTWNLDYIHGVGMYALKDGYIIKVYDKDKNIIWDAE
                     NHDMTLCHQIMKDIHTNMEKKRPGLKGSFSTYHYTLSQDNKIAGYADISYYGPYYFSE
                     SDFQFLDSLNQILFITGISSLILAVIAGMLLAKRISVPLTKAKETAEKIAEGNYQIRY
                     GADKTNAAALELAQLGQAIDHMAENLEKQEDVRRQLTSDVAHELRTPVANVSSQLEAM
                     IEEVWEPTKERLQSCYDELGRISGIISDLEKLRQIEASNMVLEKEPVDLLELAQAVKT
                     AFEPELAKKKLTCMVTGESAIISGDQKRLHQVIFNLVSNAVKYSTESGQILIDIHNLG
                     KNVQLTVKDQGIGIAKEDIPLIFERFYRTDRSRNRKTGGAGIGLTIVKAIVLAHGGNI
                     SVESVKGCGSCFTVVLPKN"
     gene            complement(6203..6667)
                     /locus_tag="ACZPCL_09075"
     CDS             complement(6203..6667)
                     /locus_tag="ACZPCL_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR1162653.1"
                     /translation="MLMPSIFGEDLFDNFMKDFPFFDDNTESNVEKKLYGRRGKNLMK
                     TDIKETEGGYELEMDLPGFTKDEIKVSLENGYMTISAAKGLDKDEQDKKSGRYIRKER
                     YAGSCERSFYVGEDITEEDIKGEFKHGILKLFVPKKEAKPAVEQKKYVSIEG"
     gene            complement(6843..6989)
                     /locus_tag="ACZPCL_09080"
     CDS             complement(6843..6989)
                     /locus_tag="ACZPCL_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162654.1"
                     /translation="MEECSVLIETTKSAEDKTSRWFDLSIDYELFRDQLGVEVFYKKL
                     ALTS"
     gene            complement(7071..7400)
                     /locus_tag="ACZPCL_09085"
     CDS             complement(7071..7400)
                     /locus_tag="ACZPCL_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR1162655.1"
                     /translation="MIYEIEVSEQADSDLRGIFEYIAFELQSPENASGQLDRLEEQIL
                     SLDTMPERYRKYEKEPWKSRGLRVLPVDNYVVLYIPDSDKKVVTILRVMYAGRDIDNQ
                     LNLHTKQ"
     gene            complement(7537..8415)
                     /locus_tag="ACZPCL_09090"
     CDS             complement(7537..8415)
                     /locus_tag="ACZPCL_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013703.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1162656.1"
                     /translation="MQIVGAVINIILDPIFIFGYFGLPAMGTRGAAIATVAGQIIAML
                     LGLYFNLTKNTDVQFDFKCIKLESYYFKGICTVGIPTIIMQSMSSVMCFGINKLLLDF
                     STTSTAVFGAYFKLQTFVYMAVFGLNNALIPIVAFNIGAKHAERIKKVIRLSGAYSAL
                     IGLVGLIIMEMLPIQLISAFAPSEEMFLLGVTALRILGLSFVFGGISVMTCYALQGFS
                     RGIASLLISALRQVIILLPLASIMGKMMGINGIWWSFLVSETVTVAFAIIYLGIAEKK
                     ELSFLASMPLEQSIAE"
     gene            complement(8486..>8869)
                     /locus_tag="ACZPCL_09095"
     CDS             complement(8486..>8869)
                     /locus_tag="ACZPCL_09095"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1162657.1"
                     /translation="VGRDITGKSVEDMVYEAQRKVILDLAGKEPCVIIGRNADFILKD
                     RDDVLNVFIHGDMPEKIQRITRLYNVGEQEAVKMMADTDKRRMANYNFYTEQNWGKAS
                     NYTLCLNSSEIGYDKCEAIIIECSK"
BASE COUNT         2474 a         2107 c         1616 g         2662 t
ORIGIN      
        1 taccaggacc ggggtacctc ttacaaagga aatataaaga gctgccagtt ctcttaacac
       61 tggtatttta aagatacgtg ccgcagccag aataaatccc agaaccagcc cccctgcaac
      121 gctcactact acaataagaa gcgttacagg gaaacgggat aagatctttg ggaaatattc
      181 caccatatcg ctgaaacgga aaatgctgga aagatccatg ttttatgctc ctgttctgtt
      241 actgttcatg ttataagatc attcataaaa tacgtgtacc gtaaatttat tcttctgagt
      301 tataatcact tcctgtgtac tcaatagaaa gctttgtaag agttccgtct tctttcattg
      361 actttaaagc agtatccatg tcatcccgga gctgttcctc tccctgtgcc agcatgaaat
      421 aggcctgtga ctcgctgaaa tagctgtctt cgttaatatc atagccaccg ccaaatgctt
      481 cattgtattt tttaaatgtc ttcttggttg caacaatggc atccagtctt ccgttcatta
      541 catcatcata aatactttca ttggtagcac cattggaata tacaatatcg attttattgt
      601 catgttctgc attgtatgtt tccagaatcg ctgctgtgtt gcttccctga ccggcactta
      661 ctttctttcc aaccagatca tcaatagagt ggatatcatc tcttcctttt ttgtatacaa
      721 gacgcttatt gtaagcggca aaaccttcat ttgtaaacag atacttcttc tctctttccg
      781 ggttcttctc aaactgctgg gagccaatgg aaaccttctt tgcatccaga gcaacagaaa
      841 tgttggcaag ttccatgttt tcaatctgga attcatactg tggaaggcgg ttgtcgatct
      901 cctgtaaaac agctacatca tagcctgcag gctgattgtt ttcatctatg taggtatatg
      961 gttcaaaagt ggcacctact gctacgtaaa cttttcttac ttcctggctg tctgaatttc
     1021 cctgggttgt attctgtcct gcatcctgag ctgccccact ctctgctgtt ttgctgctgc
     1081 agccagctaa aactgctgcc gcaagtacag ctcctgcaaa tattctgatg cctgacttcc
     1141 attttcctct cataattgct gcctcctgat ctttcatgat ctcatctatt ctgttctcac
     1201 tttttccaac ctatctaatc tgttatttct cttctttttt atcgtcctca tcatcccata
     1261 aaggctgctg taaaagccgc ttataataag ctcttgtctg atacagcgca cctaccgggt
     1321 tgtgggccag aacacggtcc tttgtgacca gcgtagttgc cagagccttg gaatatttat
     1381 aaaataaact gtcatggcct acacaaagcc ccactactac atttaactgg gtccccgctt
     1441 tatttaaaag ctttgcctgt aaaataggat tacacatcac cggtccggtc ttacatgtat
     1501 atttgggatc cagaccaata tctgttttat ctacagaacc taccttacat gccactccgt
     1561 atacatcaaa gccatttaat ttcagtatcc ttgcgaaaat acggctctct tcgatcaggc
     1621 ccacacaggt tgcgatgccg atcttctttg caccgatcct tttcgctact tccatgatct
     1681 cctctacacg ggtatatttt ccataaaagc caccttccac ttcagctgaa gcaatggcta
     1741 tcttattgtt ttcttcattt tctgtataaa gcttaactac tttttctaat tctttttcag
     1801 tcagtttttc tgtaagacaa aattccggat aagaagcgtt ccgcttggaa cagttaagca
     1861 caccacagtc cgtacagctg tggggaaata tctttttctc tgcacttact gacacttcct
     1921 gagttttttc cacatttttc ttagccttac tgctttttac cttgctatct ttttctttct
     1981 tgtcactcat atgctttctc ctcgcctttt attaaccaat cacattggta tgttaatggg
     2041 tattatagag atggtgagca tttttgtcaa caagtgattt tctattgcca gttataaaag
     2101 aagactatag cagaacaatt gcacaaaatg tctgttccac ataaaaatca caaatttctc
     2161 tggtatactc acaaaaacaa tgaaagtaan nnnnnnnnna atgtttatga agatcctcag
     2221 aaaaacactg catatcggcg gcatgagctg tgtaaactgt gaacacagga tccggtacgt
     2281 cctgaaaacc gcacccggga tccaggacgc tactgtaagc tacaataccg gccttgccca
     2341 tgttacttac gataccgata ccatttcctt aaaacagatc cgggagatca ttgaaaaaaa
     2401 cggctatacc actttgccgg aaacaggaac atccgattat gatatgaaaa aaaactttgt
     2461 ctcactggct gttatcctgt ttttatatgc ccttttacag catttcggca ttctcaacct
     2521 gctggctcca gggcagctgg cagatacaaa aatgggctat ggcatgctgt ttgtgatcgg
     2581 acttattaca tctgtacact gtattgccat gtgcggcggc atcaacctgt cccagtgtat
     2641 tccacaacct tcaaatgcct ctgatgtttc cggaagcagc cgcgctcttg acatttttct
     2701 tccggctctt ctttataata caggccgtgt gatttcttat acagcgattg gttttctttt
     2761 aggtcttgtt ggcatgctgt taggcgccgg tacacagacc gggatttcct attttctcca
     2821 gggtagctta aaaatgatcg ccggaatttt tatggtgatc atgggcatca atatgctggg
     2881 gatctttccc tggcttcgtc cacttacctt acggatccca cgttttttat ccacaaaagt
     2941 ccttaaaaag cgtgcttcca gccgacagcc actgtttgtt ggacttttaa acggcctgat
     3001 gccctgcggt ccccttcagt ccatgcagat tgtggctctg gcctgccaaa atccattttc
     3061 cggtgcttta tccatgtttt tattcagtct tggaaccgtt ccccttatgc ttgggcttgg
     3121 ctctctggtc tccgttcttg gaaagcgttt tgcaaaaaaa atgaccactg ttggcgccat
     3181 ccttgtagtt gtcctcggtc ttgccatgtt ttcccaggga actgcattag ccggtatttc
     3241 cgaaggcccg gaaaattctc aaaatacagc aaatcctcaa aatacgggaa cttccgaaga
     3301 tcagggaacc tcgacagata ccacagattc ttccaatgct gccgataaaa caccccagga
     3361 tgaaaatgca aataacatcc aggtcatcta cagcacctta cagcgcggaa gatatcctga
     3421 tatcaaagtt cagaccggtg ttcctataaa atggatcatc aatgctccca aaggctccct
     3481 taacggctgc aactacagaa tgttgctgcg ctccttcgga attgaacata ccctttcaga
     3541 aggagaaaat gtgatcgaat ttactccttc taaatccgga acttttcaat atacttgctg
     3601 gatgggaatg atacgtggta atatcactgt aacagataca ctagaaactt ctccaactga
     3661 tccagataca agtacaggag cggaagaaac agatcctgaa acagaaccat tttttaacgg
     3721 ctccggtatg ggctgctgcg gaagctaaaa acatctggaa ttgtatggcg aaaacttgta
     3781 atgaggatgt ttaagaagga atggatgatt gatatgatgg aacagaaaac gatactgatc
     3841 gtcgatgatg aaaacatgat gcgtgaggcc gttgcctcct atcttgaaaa gcagggatat
     3901 catgtgctcc aggcagaaac aggaacgcag gcactcagcc ttctggaaaa agaaacggtt
     3961 tcctttgtaa tcctggacct gatgcttcca gatatatccg gtgaagaaat ctgttccaga
     4021 atacgcaggc agtcccggat tcccattatc atgctcacag ccaaaaccat ggaagatgat
     4081 atgttaaatg gtttaaacct tggagcagac gattatatca ccaagccttt cagcctgaaa
     4141 aacctgtatg ccaggatcca ggcagtatgg cgcaggtcag accatgacct gaagcctctt
     4201 gcagaaaaat tttcatggaa tgccggggat ctggtgatcg actataaccg gaaagaagtg
     4261 ttaaaaaaag gggaaactat ttcacttacc cccattgaat ggaagatctt atctgcattt
     4321 accagatatc ctcaaaaagt atttacaaga gacgaactga ttgcttccgc tttcgacacg
     4381 gatttttccg gatatgaccg ggtgattgac acccacatta aaaatctgag aaagaaaata
     4441 gaagatgacc ccaaaaatcc catttatctg tgtacagttc atggaattgg atacaagttc
     4501 gggggaaata tacaatgaag cagaaaaaac ataagataag cacacaatta tctgccggat
     4561 ttgcagtaat cgtcctgatc actatttccg taatcagtct cactgccaat ctgctgatca
     4621 gccatcagtt tgagaaatat gtagccagac agagggagac ctttgccgaa cagctgatca
     4681 ccacacttct gtcccagtat aacagcagca accagacctg gaacctggat tatatccatg
     4741 gtgtgggaat gtatgcttta aaagacggat atatcatcaa agtgtatgac aaagataaaa
     4801 atatcatctg ggatgccgaa aatcatgaca tgaccctatg ccaccagatc atgaaagaca
     4861 tccatacaaa tatggagaag aagcggcccg gccttaaggg cagtttttcc acttatcact
     4921 atacattaag ccaggacaat aaaattgccg gttatgcaga tatcagctac tacggccctt
     4981 attatttcag tgaaagtgac ttccagtttc tggactcttt aaaccagatc ctgtttatca
     5041 ccggtatttc ttctcttatc cttgcagtga tcgccggtat gctccttgca aagcggattt
     5101 ctgttcctct tacaaaggca aaggaaacgg ccgaaaagat cgctgaggga aattatcaga
     5161 tccgatacgg agcagataaa accaatgccg cagccttaga actggcccag cttggccagg
     5221 ccattgacca tatggctgaa aatcttgaaa aacaggaaga cgtccgcaga cagctgacct
     5281 ccgatgtagc ccatgaactt cgcaccccgg tggcaaatgt gtcttcccag ttagaagcca
     5341 tgatcgagga agtctgggaa cctacaaaag agcggcttca aagctgctat gatgaactcg
     5401 gccggatctc cggtatcatt tctgacctgg aaaaactgcg ccagatcgag gcttccaata
     5461 tggttctgga gaaagaacca gttgaccttt tagaattggc tcaggcagta aaaactgctt
     5521 ttgaaccgga actggcaaaa aagaaactca cctgcatggt aacaggtgaa tctgctatta
     5581 tctccggaga ccagaaacgg ctccaccagg tgatcttcaa cctggtatcc aatgctgtta
     5641 aatattccac cgaaagcggt cagatcctga tagatatcca caatttaggc aaaaacgtgc
     5701 agctgaccgt caaagaccag ggcatcggta tcgctaaaga agatattcct ctgatctttg
     5761 aacgcttcta ccgtaccgac cgctcccgca accgcaaaac cggcggcgca ggtattggtc
     5821 tgaccattgt aaaagccatt gtgctggcac atggtggaaa tatttctgtg gaaagtgtaa
     5881 aaggctgcgg aagctgcttt acagtcgtat tgccgaaaaa ctaaatccca cttttattta
     5941 ttctggcact gatgttcccc acagtgatac tctccgcagg aatgtccctc tccatgctca
     6001 tgatgagtgc agtgcacatc cgggttatag tctaattttc cttctgcata tgcttttatt
     6061 tgtctggcgg agctgtcggc ccttttacag ctgccggtgc agcaaaaagc cgccggaaaa
     6121 agcttttaca cttcttccgg cggctcttat ttataacctt atgaccttat ttagttgacc
     6181 agctcccgct ccctgtttca tattaacctt caatagaaac atacttcttc tgctctactg
     6241 ccggctttgc ttccttcttc ggaacgaaca acttcaggat accatgtttg aactcaccct
     6301 tgatatcttc ttctgtgatg tcttcgccta catagaagct tctctcacaa gatcctgcgt
     6361 aacgctcttt acgaatgtaa cggccagatt tcttatcctg ttcgtcttta tcaagtccct
     6421 tggctgcact gatggtcata taaccattct ccagggatac cttgatctca tcttttgtaa
     6481 atccaggcag atccatttcc agctcataac cgccttcggt ttccttgata tcagtcttca
     6541 tcaggttttt acctctgcgg ccgtacagct tcttttctac attgctttct gtgttatcat
     6601 caaaaaatgg aaaatccttc ataaagttat caaataaatc ttctccaaaa atactaggca
     6661 ttaacataag tcatctctcc ttttcctata ctctctatgt tttattttac ttgttatctt
     6721 ctggaaccgg ggatgtctgg aaacttcccg gaacctttgg catcttactt ctttgcctct
     6781 ttgttccttt ctgtttccct tcctttgttc tagctgtaat ataacactta ttattagcaa
     6841 tgtcaagagg tgagtgctaa ttttttataa aaaacttcca caccgagctg gtcacggaac
     6901 agctcataat caatggataa atcaaaccat cgagaagttt tatcctctgc tgatttggtc
     6961 gtttcaatca atacgctaca ttcttccatc gtaattcagt cctttcttct gattttgagt
     7021 atgaaaaaag cagtcgatca taacggaaag actgctttaa accatatcct ttattgcttt
     7081 gtgtggagat ttaactgatt gtctatgtcc cgtccggcat acattactct aagtattgtt
     7141 acaacctttt tatcgctatc tggaatataa agtaccacat aattgtctac tggtaataca
     7201 cgaagtccac gacttttcca cggttctttt tcatattttc gataacgctc tggcatcgta
     7261 tccagactta atatctgttc ttccagacgg tcaagttgtc cgcttgcatt ttccggcgat
     7321 tgtaattcaa aagcaatata ttcaaaaatt cctctcaaat cactgtcagc ctgttcggat
     7381 acttctattt catatatcat aagccataat ccttgcgaat gtcagcacaa tagcaatact
     7441 aattttatgc tccaataatc ttattgaata tgttcacata taataatgag gtcacattcc
     7501 atgacctcat cactttcatt ttttccagat ctgccattat tcggctatac tctgttccag
     7561 cggcatagag gccagaaatg acagttcttt cttttcagct attcccagat aaatgattgc
     7621 aaatgcaact gttactgttt cactgacgag aaagctccac catattccat tgatccccat
     7681 catcttaccc ataattgatg caagtggaag caaaatgatc acctgcctta gcgcggaaat
     7741 taaaagactg gctattcctc ttgaaaagcc ctgtaaagca tagcaggtca taacagaaat
     7801 tcctccaaac acaaaactaa gtcccaaaat acgcaatgca gtcacaccaa gtaaaaacat
     7861 ttcttctgat ggtgcaaatg ctgatattaa ctgtatcggc agcatctcca ttatgatcaa
     7921 accaaccagt ccgatcaaag cagaatacgc cccgctaagc cggataactt tcttaatccg
     7981 ttctgcatgt tttgcgccaa tgttaaatgc tacgatcggg atcaaagcat tattcaatcc
     8041 aaaaaccgcc atatacacaa atgtctgtaa tttaaaatat gctccaaata cggctgtcga
     8101 agttgttgaa aaatccagaa gaagtttgtt aatgccaaaa cacataacgc tggacatgga
     8161 ttgcataata atcgttggaa ttccaacagt gcaaatcccc ttaaaataat agctttctaa
     8221 tttaatgcat ttaaaatcaa actgcacatc tgtattttta gtcagattaa aatataatcc
     8281 caacagcatt gctatgatct gacctgccac tgtagcaatt gccgcacctc tggttcccat
     8341 tgccggcaaa ccgaaatatc caaaaataaa gatgggatct aaaataatgt tgatgaccgc
     8401 accaacgatc tgcattactt aacattaatt aatcctggat cattcatatg caaaaccata
     8461 aataatccag gatcatctat actttttatt tactgcattc tatgataatt gcttcacact
     8521 tatcatatcc aatctctgaa ctgttcagac acaacgtata attgctggct tttccccagt
     8581 tctgctccgt ataaaagttg taatttgcca tacgtctttt atctgtgtct gccatcattt
     8641 taacagcttc ctgctctcct acattgtata aacgggtgat acgctgtatt ttttccggca
     8701 tatccccatg aataaataca ttcagcacat cgtcccggtc tttcaggata aaatcagcat
     8761 ttcttccaat gatcacgcag ggctccttcc cagccagatc taaaataact tttctctgtg
     8821 cctcatacac catatcttcc actgattttc ctgtaatatc acgtcctac
//
LOCUS       DBMDUA010000115         8633 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3723_length_8633_cov_2.637834, whole genome shotgun sequence.
ACCESSION   DBMDUA010000115 DBMDUA010000000
VERSION     DBMDUA010000115.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8633)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3723_length_8633_cov_2.637834"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     regulatory      complement(230..306)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(357..1757)
                     /locus_tag="ACZPCL_09100"
     CDS             complement(357..1757)
                     /locus_tag="ACZPCL_09100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1162658.1"
                     /translation="MAKKIKIRKVSAWTMGITVVLAIMFAIISYKSEKEFRTLRMTTE
                     QYIACEKAAKQLQNGSAYLTEQVRLYAITRESKYMDLYFAETNSHRRENAVESLKQYF
                     DGTEIFDSLEEAMEYSSELMNTEYYAMRLVSEALSVPEDTWPEAIKNVQLSEEDAHLG
                     RDGKLIRAGNMVCDDDYETMRTRINSDVSRCMNGLISQTRNRQGRATTIFSDMYMKLE
                     IGIVLMLVIMVFICLMLRFLIVRPLVSYNESIKKGEIFPAIGAAELQNLANTYNRVYL
                     ENQETQKLICHQAEHDALTEALNRGSYEKLLHIYETGDALFALILIDVDIFKSVNDIY
                     GHAVGDAILQKVTGSLKKAFRSIDYVCRIGGDEFAVIMVDVTSDLKYTIEDKLKYVGE
                     ELAKTEGEVPAVTLSVGVAFSDRKNPGESIFKDADKALYHVKENGRNGCAFYNGASSL
                     ENAMENHEMKDHGMEN"
     repeat_region   2297..2630
                     /inference="COORDINATES: alignment:CRISPRCasFinder:4.3.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2297..2325
                     /rpt_unit_seq="aggttcacccccgctcacgcggggaatac"
     gene            complement(2676..3575)
                     /gene="cas2e"
                     /locus_tag="ACZPCL_09105"
     CDS             complement(2676..3575)
                     /gene="cas2e"
                     /locus_tag="ACZPCL_09105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HIR1162659.1"
                     /translation="MIVIMLSDCPPKVRGDISKWLCEINTGVFVGNVSSRVREEVWQR
                     ICENIKSGQATMVFSAPGEQKMDFRVHNTTWEPVDLDGIKLMRRPLPSARASKMSEKE
                     DKGISGAKSRAERLYMADRMAKARARKKFQEGFVVLDIETTGTSPEKDEIIEVGALKI
                     EAGTVTEEFSMLIQIKGEIPIEIQKLTGITEQELQSQGRPLDEVLNKLFEFAGNRVII
                     GHNVMFDYNFIRIACRKLGIEMQLGTASKDTLALSRRKIKGVGSYQLEALMKHLGYEV
                     SNAHRALPDCYLTYQLYQKLNEM"
     gene            complement(3572..4516)
                     /gene="cas1e"
                     /locus_tag="ACZPCL_09110"
     CDS             complement(3572..4516)
                     /gene="cas1e"
                     /locus_tag="ACZPCL_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HIR1162660.1"
                     /translation="MSEMPGMIRPELQALPQIRDRMTFLYFEHCKLNRQDSAITVTDD
                     QGIIHVPAASISVLLLGPGTTVSHRAMELIGDAGVSVVWIGEHGVRYYAGGRPLTHRS
                     AFLIRQAECVSNQRKHLMIVRKMYQLRFPEENSSKMTLQQLRGREGSRVRSVYRKYAK
                     EYDVKWNGRDYDPEHFETGDPVNQALSAGCACLYGLAHAVIFALGLSPGLGFIHVGHD
                     CSFVYDIADLYKSEVVIPVAFQVASEEPEDLPGIVRRKVRDVMTEKHILERMVKDIRW
                     LFLAEDEKKEDAVEAVYLWDDKEGTVRNGRSYGQEEWE"
     gene            complement(4580..5218)
                     /gene="cas6e"
                     /locus_tag="ACZPCL_09115"
     CDS             complement(4580..5218)
                     /gene="cas6e"
                     /locus_tag="ACZPCL_09115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HIR1162661.1"
                     /translation="MYLTRMELNTKNMDTIRLLGSQEKIHGLVESGFLGERKRNLWRL
                     EYLGDRLYLLILSPDRPDLSIGVRRYGFPDDENAYQTKDYTPLLERVKNDTWWHFKLV
                     ANPTRSVASTPGERGKVKAHCSLKYQEEWLLDRAQKHGFELLPENFQIVERDWHIFRK
                     GKEQGKNKVSIKGVTYEGILKVYDEEKFKELLVAGIGRGKAYGMGLLTIVHI"
     gene            complement(5233..5940)
                     /gene="cas5e"
                     /locus_tag="ACZPCL_09120"
     CDS             complement(5233..5940)
                     /gene="cas5e"
                     /locus_tag="ACZPCL_09120"
                     /inference="COORDINATES: protein motif:HMM:NF021240.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HIR1162662.1"
                     /translation="MSTLLLRLAGPVQSWGIDSKFEVRRTENAPSKSGVTGLLAAALG
                     IQRNEDISSLNQLRLGVRTDQEGRLLKDFHTAHSEKNSYITTRYYLSDAIFLVGLECE
                     DKGFLQKLEYALKHPAFPLFLGRRSCPPEAGMVLGIRDLPLETALEEEPWQGPEWKKE
                     KMPLKLPMFIECAPDDPTGGMVKDKAESFDPYQRRYGYRRMKRTEWNVSDRVISDKMT
                     AGKNPDIVREHDAMGEL"
     gene            complement(5937..7004)
                     /gene="cas7e"
                     /locus_tag="ACZPCL_09125"
     CDS             complement(5937..7004)
                     /gene="cas7e"
                     /locus_tag="ACZPCL_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009533382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HIR1162663.1"
                     /translation="MDEKRLYVDLHVVQTVPPSCINRDDTGSPKTAVYGGTTRARVSS
                     QAWKKAMREYFSQLFHEQNIGSRTKKVIEMVAKEIRLQAPDEADAEKKAAEALKNAGI
                     KINDKKGESDALLFMSKGQAKALAQLVVSGNTDKKAYKEAMQKEPSYDMALFGRMVAS
                     DPSLNYDAAAQVAHSISTHTIHNEYDYFTAVDDCSQEDNAGAGHLGTVEFNSFTMYRY
                     ATVNVMELYRHLGKDTAEVVEGFIKAFICSMPTGKQNTFANRTLPDAVYITVRKDQPL
                     NLCGAFERPILSNCGYVDKSVEAMTAYAKKVYDNYADEPEKAFGIGDQLEGAAEVMSL
                     KQLLESVKTEIQAQLDDGGEE"
     gene            complement(6997..7609)
                     /gene="casB"
                     /locus_tag="ACZPCL_09130"
                     /gene_synonym="cse2"
                     /pseudo
     CDS             complement(6997..7609)
                     /gene="casB"
                     /locus_tag="ACZPCL_09130"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02548.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
     gene            complement(7625..>8633)
                     /locus_tag="ACZPCL_09135"
     CDS             complement(7625..>8633)
                     /locus_tag="ACZPCL_09135"
                     /inference="COORDINATES: protein motif:HMM:NF021030.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HIR1162664.1"
                     /translation="KGKGLPSPGVGWPGKLGLIAAVGDNLFQTLMLNLTLLKDGRSLW
                     EGEDKPIWEREPSKKERQEIAVPDNLAELLTLQSRRLLLKRDHNKVVGYYLLGGDFFD
                     KNLAYAEQMTVWREVKEKDRKFFTPRRHDPSRQMWRDFGSIFVESGSNVRKPGVVSWY
                     DTIATEMQWKIRPIRFRIASVQYGDKDFFVNDTFSDSLAFQGELLLQMSRPWQSRILD
                     EINKCEEVAGAIGMLEGNLARAEGRDADIVYAKELFYDRIDQPFRRWLMEILPETDDI
                     SKTCKKLDEIIQKVGKELGEEMVSNAGEAAFAGRTKDDVYYASPEAYNWFLGKLHKIY
                     H"
BASE COUNT         2157 a         2282 c         1457 g         2737 t
ORIGIN      
        1 agggccccag taaaatacct gtgaccaaca tgccaatgat acggggtatc ttaaatttct
       61 ggcacagcgc cgccatggca agacccagta aaaatacaaa tgatagtgat gttaacattt
      121 tcattttcct cctttaatag cacggatccc caaacctgat caattcatat actgtcagca
      181 gatcaacagc ctccattgca acaaaaaaag ctggtgactc cgtgaatgtt tcatcacaga
      241 agtcatcagc ttacatttct aagcggttca ggtttccctt gggagaactt cattcccggc
      301 gcttattata aaacataacc atctgattgt aaatatttat tttcagatcg tccgttctaa
      361 ttttccattc catgatcttt catttcatga ttttccattg cattttccag ggagctggca
      421 ccattataaa aagcacagcc gttacgtccg ttttccttta catgataaag ggccttatct
      481 gcgtctttga aaatgctctc acccggattt ttcctgtcag agaatgccac acctacgctt
      541 aaggttacag ccggaacttc tccctctgtc tttgccagtt cttccccaac atacttaagc
      601 ttatcttcaa tggtatactt caggtcactg gtcacatcta ccatgatcac ggcaaattca
      661 tcaccgccaa tacgacagac atagtcgatg cttcggaatg cttttttcag gcttccggtc
      721 actttctgta aaatagcatc tcccactgca tgtccatata tatcatttac ggatttaaaa
      781 atatccacat caataaggat caaggcaaaa agcgcatctc ctgtttcata aatatgaagg
      841 agtttttcat aggaacctct gtttaatgcc tctgtaaggg catcatgctc tgcctgatga
      901 cagatcagct tctgggtctc ctgattttcc agataaactc tgttataagt atttgcaagg
      961 ttctgcagtt cagcagcgcc gatcgcaggg aatatttccc ctttttttat gctttcgtta
     1021 tagcttacaa gcggtctgac aataagaaat cttaacatca gacagataaa gaccatgatc
     1081 accagcataa gaacaatgcc gatctccagt ttcatgtaca tatcagagaa aatggtagtt
     1141 gctcttccct ggcgattcct cgtctggctg atcagtccgt tcatacaccg tgatacatct
     1201 gaattgatcc gtgtccgcat ggtttcataa tcatcatcac agaccatgtt acctgcccgg
     1261 atcagttttc cgtctcttcc caaatgggcg tcttcttcac ttaactgtac attttttatg
     1321 gcttccggcc aggtatcttc cggtacggaa agtgcctcag acaccagacg catggcataa
     1381 tattccgtgt tcatcagttc actggaatat tccattgctt cctctaagga gtcaaatatc
     1441 tctgttccat caaaatactg cttcaggctt tccactgcat tttccctgcg atggctattc
     1501 gtttctgcaa aatacagatc catatacttg ctttcccggg tgatcgcata caggcgaacc
     1561 tgctctgtca gataagcaga accattttgc agctgttttg ccgccttttc acaggcaata
     1621 tactgttctg tagtcatgcg aagcgttctg aattctttct cactcttata agaaatgatc
     1681 gcaaacatga tcgccagcac tacagttatc cccatagtcc atgcgcttac tttccgtatc
     1741 ttgatctttt tagccatagc cgtacccgcc tttttaatgt ctgatatacc tgtctttcaa
     1801 ttactttctt ctgtccgttt tctcccatac atatcccctg gcaattttca taggatgcat
     1861 aacaatgcca aaagagctgc cggccttatt aaaggcttgg cagctcgcac attcacggca
     1921 gattttctcg ccacttctcc ttaaaacact aaattgattt tatcacagca ggctgggggg
     1981 agtcaaggct ggatagagat acaactttgt agactttgct ttcttttgtt gattttagcc
     2041 ccgcggatgc ggggaatact atatctctgg aatttaatca gggagcatcc ctgcgtacgc
     2101 aaggactata gataagcttc tcttttgtct acccatacac taacaatatt gagttggttt
     2161 gtcaagagat taatgctgct tttacaaaga gcatctgcac ataagaaatg cttaaatttt
     2221 acagactggg caattagatg ttgctttgtt tatgcgggga aatacaacca ccagtaactt
     2281 tttccggctg ctacctgggt tcatccccgc tcacgcgggg aatacagtcc cctatgggca
     2341 tgttcccggc ttatgccagg ttcacccccg cccatgcggg gaatacgaga tccatgatct
     2401 cacaaaagca ctcatccaag gttcaccccc gctcacgcgg ggaatacata gccagaagaa
     2461 gaaacaacgg cgactgagta ggttcacccc cgctcacgcg gggaatactt aggctcatat
     2521 attttaaatt cattgtacgg aggttcaccc ccgctcacgc ggggaatacc aacaacttca
     2581 agttctccac cttcaccgat caggttcacc cccgctcacg cggggaatac actaaaaaaa
     2641 gccttatttt atctcacttt ttccaacacc cattttcaca tttcatttaa cttctgatat
     2701 aactgatatg taagataaca gtctggtaac gccctatgtg catttgaaac ttcatatccc
     2761 agatgcttca taagtgcttc cagctggtaa cttcccaccc cctttatttt tctccgcgac
     2821 agtgccaatg tatctttgga agcagttccc agttgcattt ctattcccag tttccgacag
     2881 gcaatgcgga tgaaattata atcaaacata acattatgtc ctataataac tctgtttccg
     2941 gcaaattcaa acaatttatt aagtacttcg tccagtggtc tcccctgact ttgcaattcc
     3001 tgttctgtaa tgcctgtcag tttttgtatc tctattggaa tttcaccttt tatctggata
     3061 agcatagaaa attcttctgt gacagtgcct gcctctattt ttaacgcacc gacttctatt
     3121 atctcatcct tttccggtga tgttcctgtt gtttctatat cgagtaccac aaaaccttcc
     3181 tgaaactttt tcctggcacg ggctttggcc attctgtctg ccatgtatag ccgctccgcc
     3241 cggctttttg ctcccgatat tcccttatct tctttttccg acatcttaga tgcccttgca
     3301 gacggaagcg gccttctcat cagctttatt ccatcaagat caaccggctc ccaggttgta
     3361 ttatgcacac gaaaatccat tttctgttct cccggtgcac taaaaaccat ggttgcctgt
     3421 cctgacttta tattttcaca aatacgctgc cagacttctt cccttaccct gctgctcaca
     3481 tttccaacga aaaccccagt gttgatctca cacagccatt tagatatgtc cccccggact
     3541 tttggcggac agtcagacag catgatcacg atcattccca ttcctcctgt ccataggaac
     3601 gtccattcct gactgttcct tctttatcgt cccataaata aacagcttct actgcatctt
     3661 cctttttttc atcctctgcc agaaacagcc atcgaatgtc tttcaccata cgttccaata
     3721 tatgcttttc agtcatcaca tctctgacct tccttcggac gatccccgga agatcttccg
     3781 gttcttctga tgccacctga aaagcaacag ggataactac ctctgattta tacagatccg
     3841 caatatcata tacaaaagaa cagtcatgtc ccacatggat aaaaccaagt cccggtgaca
     3901 ggccaagcgc aaaaatcact gcatgggcca atccgtacag acatgcacag cctgctgaca
     3961 atgcctgatt taccggatct cctgtttcaa aatgctccgg atcataatct cttccgttcc
     4021 attttacatc atattccttg gcatatttac gataaacaga tcttactctg cttccttccc
     4081 ttccacgcag ctgctgaagt gtcattttag aagaattttc ctctggaaaa cgcagctgat
     4141 acatctttct tacgatcata agatgttttc tctggttact tacacactct gcctgtcgga
     4201 tcagaaaagc ggacctgtga gtaagaggac gtccgcctgc atagtacctg acaccatgtt
     4261 ctccgatcca gaccacgctg acaccggcat ctccgatcag ctccattgca cgatggctca
     4321 ctgttgtccc cgggcctaaa agcagtactg aaatagatgc tgcaggaaca tggatgatac
     4381 cctgatcatc cgtaactgtg atcgcactgt cctgacggtt taatttacaa tgttcaaaat
     4441 acagaaacgt cattctgtct ctgatctgag gcagtgcctg taactcagga cggatcattc
     4501 ccggcatttc actcatatgt ttctccttat ttggcagttt actgcaaaag aaaactcaaa
     4561 aagcacccca tccgcagact tatatatgaa caatggtaag caatcccatt ccataagctt
     4621 ttccacgccc gataccggct accaaaagct ctttgaattt ttcttcatca taaaccttca
     4681 aaattccctc atatgtcacg cctttaatac tgaccttgtt ctttccctgt tcctttcctt
     4741 ttctgaaaat gtgccagtct ctctcaacga tctgaaaatt ttccggcaga agttcaaagc
     4801 catgtttttg tgctctgtca agaagccatt cttcctggta cttaagagaa caatgagctt
     4861 ttacttttcc ccgttctcca ggcgtgcttg ccacacttct tgtaggattt gccaccagtt
     4921 taaaatgcca ccaggtgtca ttttttaccc gttccagaag tggtgtgtaa tctttcgtct
     4981 gataggcatt ctcgtcatca gggaaaccat acctgcgcac tccaatgcta agatccggcc
     5041 tgtccgggct taatatcaac agatacagcc tgtctcccag atattcaagt ctccataaat
     5101 ttctctttct ttctcccaaa aatccacttt caacaagtcc atggatcttt tcctgtgagc
     5161 ctaaaaggcg tatggtatcc atattttttg tatttaattc cattctggtc aaatacatat
     5221 atttttatca cctcacaatt ctcccatggc atcatgttct cttacaatat ccggattttt
     5281 gcctgcagtc attttgtctg aaattaccct atctgatacg ttccattccg tccgtttcat
     5341 ccttctgtaa ccataccttc tctgatacgg atcaaaagac tctgccttat cttttaccat
     5401 gccgcctgta ggatcatctg gcgcacattc aataaacatg ggaagtttta aaggcatttt
     5461 ttcctttttc cattcaggtc cctgccatgg ttcctcttct aatgctgttt ctaatggcag
     5521 atcccgtatt cctaaaacca taccagcttc cggcggacag gatcttcttc ccagaaaaag
     5581 gggaaatgcc ggatgtttca gggcatattc caatttctgt aaaaatcctt tatcctcgca
     5641 ctctaatcca accaaaaata tagcatctga cagataatat ctggtggtta tataggaatt
     5701 tttctcacta tgggctgtat gaaaatcctt taaaagtctt ccttcctgat ccgtcctgac
     5761 tcccagtctc agctggttta aactgctgat gtcttcgttt ctttgaattc ccaaagctgc
     5821 tgccaacagt cctgtcactc cactttttga aggtgcattt tccgtccggc gaacttcaaa
     5881 ttttgaatca atcccccatg actggacagg tcctgccaag cgaagtaaca atgtactcat
     5941 tcttctcctc catcatctaa ctgtgcctgg atctctgtct ttacgctttc caacagctgt
     6001 tttaaagaca taacctctgc ggctccttcc agctgatcac caatgccaaa tgccttttcc
     6061 ggttcatctg cataattatc atatactttc ttcgcatatg cagtcatagc ttccacagac
     6121 ttatctacat agccgcagtt gcttaagatc ggacgctcaa atgccccaca aagatttaac
     6181 ggctggtcct ttcttactgt aatgtatact gcatccggca atgtacggtt tgcaaaggta
     6241 ttctgttttc ctgtaggcat agagcagata aatgccttta taaatccttc tacaacctct
     6301 gccgtatctt ttccaagatg gcggtacagt tccattacat ttaccgttgc atagcgatac
     6361 atggtaaatg agttaaattc cacggttcca agatgacctg cacctgcatt gtcttcctgt
     6421 gagcagtcat ctactgccgt aaaataatca tattcgttgt ggatcgtatg ggtagaaata
     6481 ctgtgtgcaa cctgtgctgc cgcatcataa tttaaagaag gatcacttgc caccattctt
     6541 ccaaacaacg ccatgtcata ggaaggctct ttctgcattg cttccttgta tgccttttta
     6601 tctgtatttc cggataccac cagctgggcc agcgccttag cctgtccttt gctcataaag
     6661 agaagggcat cactttctcc ctttttatca ttgatcttga tgccggcatt tttaagagcc
     6721 tctgctgcct tcttttctgc atcagcttca tccggagcct gtaaacggat ctccttcgct
     6781 accatttcaa tcactttttt cgttctgctt cctatgttct gctcatggaa caattgggaa
     6841 aaatactctc tcatagcctt tttccatgcc tgtgaagaaa ctctggctct cgtagtacct
     6901 ccatatactg ctgttttagg acttcctgta tcatctctgt ttatacagct tggcggaact
     6961 gtctgtacca catgcagatc cacatataat cttttttcat ccattttcct tttcctcctc
     7021 attttctgct tttacattac agtatctgta aaaatctcgt ccccatttta accggatctc
     7081 cggaattttt tccaaatcct gatacagata aaaatccact gctaactgca cataatccag
     7141 acggatctcc ttctgcctga ataactgcac cattcccttc atatgccatg ctaattccgt
     7201 cagatcggat gatacagctg ctgcattaaa ccttctgcgt actgctttta atgactccag
     7261 atcgttgtgc tgccatttct cctgctgctc tgccaaagcc aattcccctt tggtgggcat
     7321 tttcatcttt aagagaacga ctttgctgat ggatcgcata cagtgtaaat gccgtatata
     7381 atgccagttc tgcccgccca ggtgtatttc ctctgcctat taattcctct ggcagatctt
     7441 caaataaaat cccccaaagt tccggatctg cacctggtac ttttccaaca cctttacgca
     7501 ttttagccag atatgccttg gttccgctct catctgggtt ttctcttaat acacggagct
     7561 tcttttccgc aaatcctttc atctgttcaa tcggattgct attttccatc ctcttcacct
     7621 cctgtcagtg ataaatttta tgtagttttc caagaaacca gttataagct tccggtgaag
     7681 catagtacac atcatctttc gttcttcccg caaaggcagc ttctcctgca tttgaaacca
     7741 tttcttctcc cagttctttt ccaacctttt gaatgatctc atctaatttt ttacaagttt
     7801 ttgatatatc atctgtttct ggtaaaattt ccataagcca tcgtctaaaa ggctggtcga
     7861 tccggtcata gaacaattcc tttgcataga caatatctgc atctctgcct tctgccctgg
     7921 caagatttcc ttccagcatc ccaatagccc cagctacctc ttcacattta ttgatctcgt
     7981 ctaagatcct gctctgccat ggacgactca tctgtaaaag cagttcaccc tgaaaagcca
     8041 gtgaatcact gaatgtatca ttcacaaaaa aatctttatc tccatattgt acagaagcaa
     8101 tcctaaaacg gatcggtctt attttccatt gcatttccgt tgctatggta tcataccagc
     8161 tgacaactcc cggctttcgc acattggatc ccgattctac aaatatgctt ccaaaatccc
     8221 gccacatttg tctggaagga tcatgtctgc gtggtgtgaa aaattttcga tccttttctt
     8281 tcacttctct ccacacagtc atctgctctg catatgccag gtttttgtca aagaaatctc
     8341 ctcccagcag gtaatatcca accaccttat tatgatctcg ttttaacaga agccgccttg
     8401 actgcaaagt aagaagttct gccaaattat caggaactgc aatttcctgc ctttcttttt
     8461 tagatggttc ccgctcccat atcggcttgt cctctccctc ccaaagactt ctcccgtctt
     8521 ttaaaagggt aagatttaac atgagtgtct gaaaaaggtt atctccaact gcagcgatca
     8581 atcccaactt tcccggccag cctactcctg gagaaggaag accttttcct ttt
//
LOCUS       DBMDUA010000116         8576 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3743_length_8576_cov_3.126490, whole genome shotgun sequence.
ACCESSION   DBMDUA010000116 DBMDUA010000000
VERSION     DBMDUA010000116.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8576)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3743_length_8576_cov_3.126490"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="ACZPCL_09140"
     CDS             complement(<1..476)
                     /locus_tag="ACZPCL_09140"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR1162665.1"
                     /translation="MKLKKWVALATAAAMCLSMTACSVSVSTNETTKAAEPSAETTAA
                     EAGDKEESGEAAESAAEGDLVATADYPTKPITMIIPYAAGGTTDVWGRKLAALLEKYL
                     GQAITVTNQGGASGSIGSQFVKEQPSDGYTVLVCAETMGTYRTMNVCDLDYSDFTV"
     gene            710..1222
                     /locus_tag="ACZPCL_09145"
     CDS             710..1222
                     /locus_tag="ACZPCL_09145"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR1162666.1"
                     /translation="MPTKLTKPIFLCGYTNSGKTTIGKELARQLDVPFYDTDELITSQ
                     TGQTPQQIFASQGNEAFRNIEHEITKQVSSYSPCVISTGGGLLTYSRNGDLLSKAGTI
                     IYLNRPFEDCYKSLMRNPDRPLIKNNTKEELLDRYNKRAVSYEAYAAFTVKNDRTPQA
                     TARHIIELLF"
     gene            complement(1228..2121)
                     /locus_tag="ACZPCL_09150"
     CDS             complement(1228..2121)
                     /locus_tag="ACZPCL_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1162667.1"
                     /translation="MKSELGKGSEITIGLRFKTTSATKNRGVIQELNGFRALVADDNM
                     DSCVSVEKMLRTIGLRPEWTTSGKEAVFRAKYATEQNDPFHVYIIDWLMPDMNGVEVV
                     RRIRSEIGDEVPIIILTAYDWSDIEDEAREAGVTAFCAKPLFLSELYDVLQNSSKAGG
                     IGGNEIIAPDTFKGRKVLLVEDVELNREIAETILQEAGIHVVCVGNGQKAVEYMSQPE
                     ADDIDLILMDIMMPVMDGYEAARRIRKLADPVHANKPIIAMTANAFEEDVKAALAAGM
                     NEHLSKPIRIELLYEIMKKYL"
     gene            complement(2145..2459)
                     /locus_tag="ACZPCL_09155"
     CDS             complement(2145..2459)
                     /locus_tag="ACZPCL_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162668.1"
                     /translation="MREEDVLQVLGGSFFNSVLVDIEKNTYTRMNFAPWLLKISQTGM
                     YTGLADFIVCNSAVEEYKESLKKDLSLESIETFFNSRDPEERDEENSITYEAEVDGKR
                     IL"
     gene            complement(2544..3884)
                     /locus_tag="ACZPCL_09160"
     CDS             complement(2544..3884)
                     /locus_tag="ACZPCL_09160"
                     /inference="COORDINATES: protein motif:HMM:NF015563.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HIR1162669.1"
                     /translation="MFFAVLSWIMPAGAYDRVLDPNTGRNIVDPNSFHYLPKTPITFI
                     QFISAYSGGFTNAASMIFMTLVVGGTFGIINELGIIPAALAIILKKFDKNREMAIPFL
                     ILVFGLFESFMGAPELCIIFLPMILPLVLDLGFDTMTALAIVICGNCVGYSTGMGNPF
                     TTIIGQKICQLPLYSAMWYRAICFFLFYGITVWYILRYAKKIRKNPILSATYEEDEIR
                     RKENMQMTQNATLSAKNKVVCVYVVVCFFFNIFGVIKLGWDIAEMTGLFLVMSTGAGV
                     LSGHSLTDTCKMFMNGARDILQGALVMCFARTIALLMTTSNTLDVFVYGISKIAGAFP
                     PTLAIVGIFIAGMLLNFPINSGSGQMTATMPIMSPLADILHVSKQGAVLATQFGDGWS
                     NTIYPTNASYMATMAVAKVNWVDWLKFQFPLHCIWTVVSVIMLIIAQTINIGPF"
     gene            complement(3959..5146)
                     /locus_tag="ACZPCL_09165"
     CDS             complement(3959..5146)
                     /locus_tag="ACZPCL_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR1162670.1"
                     /translation="MKITKVDIYMLDATAQRASRRPICCRVWTDEGFYGDGEAGIAFD
                     YAAPAGIGMLQDISRLVIGKDPMRTDELWERMFKVSFWGQGGGPVVFAAISAIDIALM
                     DIKGKVLNVPVYELLGGKVNDSIRCYASQLQFGWNEDVGPRGTAKEYADICKYAMEDG
                     YDAVKLDFTLYDRDKKDIPNRDCEGFVSTDFYNMVEERIVAIREACGNVDIIMENHGR
                     TDVTSGIKLGELCDKYNFYALEEPCTPLRPEFQKLIREKVRTPLASGECLYTRWQFLN
                     YFKDNSIQLVQPDACNCGGISECRKICTMAEAFDVKAQIHCAGGPISTAAALQLTAAT
                     TNFAIYEHHFRSTQPAIARLGKYDYQPVNGKFYVSDRPGIGQELSEAAIAEALVHVTV
                     DEP"
     gene            complement(5186..6337)
                     /locus_tag="ACZPCL_09170"
     CDS             complement(5186..6337)
                     /locus_tag="ACZPCL_09170"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR1162671.1"
                     /translation="MKIDFNRQINRKNTDCSKWDGQGGDYIPLWVADMDFPVAEPVLE
                     ALKKRLEHPFFGYTFPGESVYQAVISYYKRRYDFDVKKEWILFNHGVNTGNNIAARAL
                     GGTIMVATPMYPHINQKLPQEAGKPVIRVPLKQENGLYTFDFEAMEKAVTQDVTTFVL
                     CNPHNPVGRVFTGKELEELFAFAARHDIVVVADEIHSDLILEGEHIPAITLNEAARQR
                     VILHHSASKTYNIPGLPVAFAIIPNEKLRHKYEEVAATMHAPFDTLSFVALEAAFTKG
                     EEWRQELLEYLRENKKWLDERISRIPGLSICHSEGTYLGWIDARETGLSDPAGFFMKE
                     AGVKFNDGESFSAPGFIRVNFACPRAYLEEAFDKVEKALGNWKKQEKVY"
     gene            complement(6502..7254)
                     /locus_tag="ACZPCL_09175"
     CDS             complement(6502..7254)
                     /locus_tag="ACZPCL_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1162672.1"
                     /translation="MIEIKNLHKAFGQNQVLKGVDITVNKGDVVVVIGPSGSGKTTML
                     RCINFLERADEGTITVGDITVDAAKAKKKEIHEIRLRTAMVFQNYNLFANKTALGNVM
                     EGLTQARGVSKEKAKEIALDALEKVGLKDKADYYPSQLSGGQQQRVGIARAVVLNPDV
                     ILFDEPTSALDPELVGEVLKVIKDIAKEGITMIIVTHEMSFASDAANRVIFMAGGVVV
                     EEGKPDEIFSNPKEERTKQFLERVLPKWSYTI"
     gene            complement(7251..7964)
                     /locus_tag="ACZPCL_09180"
     CDS             complement(7251..7964)
                     /locus_tag="ACZPCL_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1162673.1"
                     /translation="MASPFEFSYVFTVIGKIWEAMPVTLLLTFAPLFIGFLFGVPFAV
                     IRRFNVPFAASFLKNASNILKGIPAVLLVLMINYLILKPIDYLALSYEWAKPLQTMNK
                     IYIGIAALSIYAVVQITETVLGALLSVGKGQYEAAYSIGLTRTKTLFRVVFPQAVPAA
                     LPVLCNNVIGLLKSTSIVYLISVNDILGAAINSAAVNFRYLEAYIAVAVVYWAMCVCV
                     EKLFYVMEKRYKYAAGGKI"
     gene            complement(7948..>8576)
                     /locus_tag="ACZPCL_09185"
     CDS             complement(7948..>8576)
                     /locus_tag="ACZPCL_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1162674.1"
                     /translation="VVSVAGGLVLGFILAAARIFKIPVLRELAALYISFVRGTPILVQ
                     LFVVYYGLPLLVAPLGVDINHWSKLFFVLVTYLLNDGAFMSEIIRSSIESVPKGQLEA
                     AASVGLTTFQTYKRIIIPQAFKIAAPAFGTRVVGSFQATAMAFTLGIIDIMGQVKAIG
                     TRTNRVLEGYVDAAIIFIIVSYLLERLFTWMEHRLNGQTRRKENGIAL"
BASE COUNT         2429 a         2190 c         1607 g         2350 t
ORIGIN      
        1 ataacagtaa aatcagaata atccagatcg cagacattca tagtgcgata ggttcccatg
       61 gtctctgcac aaactaatac agtataacca tcagaaggct gttcctttac aaactgggaa
      121 ccgatagaac cagaagcacc accctggttg gtaactgtaa tagcctgtcc taaatacttc
      181 tctaacagag ctgctaactt tctgccccat acatccgtag ttccgcctgc tgcgtaaggg
      241 atgatcatag taataggctt ggtaggataa tcagcagttg ctactaaatc tccctctgct
      301 gcagactcag ctgcttcgcc ggactcttcc ttgtcccctg cctctgctgc ggtggtttct
      361 gctgatggct cagctgcttt tgttgtctca tttgtggata cagaaacact gcatgctgtc
      421 atagacaggc acattgcagc agctgtagcc agtgcgaccc attttttcag tttcataata
      481 ctacactcct ctttctgtaa aataatatac tctcgtaatc tccgctgtgc taatgcagat
      541 tataacacag caggtgacag gtttcaacaa taaactttgc caagttaaaa ctttaaagct
      601 tttactcact gttgtcatat tccttgtatt ctttcctgaa acatggtatc atttttgctg
      661 tatacaacct caaagtaatt ttttataccc acaggaggat ccttaaaaaa tgcccacaaa
      721 actgacaaaa cccattttcc tctgcggcta caccaacagc ggaaaaacta ccataggaaa
      781 agaactggcc cgccagcttg acgtaccatt ttacgataca gacgaactga tcacctccca
      841 gacaggacag actccacaac agatcttcgc ctctcaaggc aacgaagcct tccgcaatat
      901 tgaacatgag atcaccaaac aggtatccag ctattccccc tgcgtaattt ccacaggtgg
      961 cggtctgctc acctactccc gcaacggcga tcttctctcc aaagccggta ccattatcta
     1021 cttaaaccgc ccctttgaag actgctacaa aagcctgatg cgaaatcctg accggcctct
     1081 tatcaaaaac aacaccaaag aagaacttct tgaccgatac aacaagcggg cagtctccta
     1141 tgaggcttac gcagcattta cagtaaaaaa tgaccgcacc ccacaggcaa ctgcccgtca
     1201 tatcattgaa ctacttttct aaaaacatca taagtatttc ttcataattt catacagcag
     1261 ctcaatacgg atgggcttag ataaatgttc attcatccca gccgccagag ctgctttcac
     1321 atcttcctca aaagcattgg ctgtcatagc aatgatcggt ttattggcat ggaccggatc
     1381 cgccagtttc cggatccttc ttgcagcctc atacccatcc attaccggca tcatgatatc
     1441 cattaaaata agatcaatat catctgcctc cggctgactc atatattcta ccgccttctg
     1501 tccgtttccc acacagacca catggatgcc tgcttcctgc aagatggttt ctgcgatctc
     1561 acggttcagt tccacatctt ctaccagaag taccttcctg cccttaaaag tatccggcgc
     1621 aatgatctca tttccgccaa taccgcctgc cttgctggaa ttttgcagca catcataaag
     1681 ttccgataaa aacaacggct tggcacaaaa tgccgtaaca cctgcttctc ttgcctcatc
     1741 ctcaatatcc gaccagtcat aagcagtcag aataatgatg ggcacctcat ctccaatctc
     1801 acttcggatc cgtcgtacta cctccacgcc attcatatcc ggcatcagcc agtcaataat
     1861 gtatacatgg aacggatcat tctgctcagt agcatatttt gccctgaaaa ctgcctcctt
     1921 tccggaagtc gtccactcag gccgcaaccc gatcgtccgc aacatctttt ccacgctgac
     1981 gcagctgtcc atattatcat ctgccaccag tgcccgaaaa ccgtttaatt cctggatcac
     2041 gccccggttc tttgttgcag aagtagtctt aaaacgcaag ccaatagtga tctcagagcc
     2101 ttttcccagc tcacttttca cctttatatt tccgcccatc atatctacaa tattctcttc
     2161 ccgtctacct ctgcctcata ggtgatactg ttctcttcat cccgttcttc cggatctcgg
     2221 ctgttaaaaa atgtttctat actttccaga gacagatctt tctttaaact ctccttatac
     2281 tcttccactg cagaattaca gacaataaaa tcagccagcc cggtatacat accagtctga
     2341 ctgattttta acaaccacgg tgcaaaattc atccgcgtat atgtattctt ttctatatcc
     2401 accaggacac tattaaagaa acttccacct aaaacctgca gtacatcttc ttccctcatc
     2461 ctaagctcct tcaatttccg tcaaaccaaa aatctcatac tgctttcatt ttaagggatg
     2521 ggctaaggat tgtccagcag attttaaaac ggtcctatat taattgtctg ggcaatgatc
     2581 agcataatga cacttacaac agtccagata caatgcagtg gaaactggaa tttcagccag
     2641 tctacccagt ttactttggc aactgccatc gttgccatgt aagaagcatt ggtaggataa
     2701 atagtattgg accagccatc accaaactgt gtggcaagga cagcgccctg cttggaaaca
     2761 tgaaggatat ctgccagtgg agacatgata ggcatggtag ctgtcatctg gccggaaccg
     2821 ctgttgatcg ggaaattaag cagcatgccg gcaataaaga tacccacaat agccagagta
     2881 ggaggaaaag cgcctgcaat ctttgaaatt ccataaacaa atacgtcaag tgtattgctg
     2941 gttgtcataa gaagggcaat ggtacgtgca aaacacataa caagggctcc ctgaagaata
     3001 tctcttgcac cattcatgaa cattttacag gtatcagtaa gactgtggcc ggagagaacg
     3061 ccggcacctg tactcattac caggaacaga cctgtcattt cagcaatatc ccagccaagc
     3121 ttgatcacac cgaaaatatt aaagaaaaag cagacaacaa catagacaca taccactttg
     3181 tttttagcgc ttaaagttgc attctgagtc atctgcatat tctcttttct gcgtatctca
     3241 tcttcttcgt aggtagcact gagtatagga tttttgcgta ttttcttcgc atatcttaaa
     3301 atataccata cagtgatccc gtaaaaaagg aagaagcaga tggcacgata ccacatagca
     3361 gaataaagag gaagctggca gattttctgg ccgataatag tagtaaacgg atttcccata
     3421 cccgtactat agccaacgca gtttccgcag ataacgatag caagggcagt catggtatca
     3481 aaaccaagat ccagtaccag cggcagtatc atgggaagga agatgataca aagctccgga
     3541 gcccccataa aggactcaaa aagtccaaaa acaagaatga ggaacgggat cgccatttcc
     3601 cggttcttat caaatttttt aaggatgatg gcaagagcag ccgggataat gccaagttca
     3661 ttgataatgc cgaaggttcc acctactaca agagtcataa agatcatgct ggcagcattg
     3721 gtaaaacctc cagagtaagc agatataaac tggataaatg taatcggggt ttttggaaga
     3781 taatgaaagc tgttaggatc aacaatattt cttcctgtat tgggatctaa tacccggtca
     3841 taagctcctg caggcataat ccacgaaagt acagcaaaga acatagcaat tcataccagt
     3901 ataacaaaaa tgtgggggat ctgtattttt ttcttcacag tatagttctc ctctccgttt
     3961 atggttcatc aacggttaca tgaacaagtg cctctgcaat ggctgcttct gaaagttcct
     4021 gtccgatacc cggtctgtct gatacataga attttccatt tacaggctga taatcatact
     4081 tgccaagacg ggcaatagca ggctgtgtgg aacgaaaatg atgttcataa atcgcaaaat
     4141 tggtagtagc ggctgtcagc tgtaaagcag ctgctgtgga aattggaccg cctgcacagt
     4201 ggatctgcgc ctttacgtca aaagcttctg ccatggtaca gatctttctg cactctgaaa
     4261 taccgccgca gttgcaggca tccggctgaa ctaactggat agagttatct ttaaagtaat
     4321 taaggaactg ccatctggta tataaacatt ccccagatgc caaaggagtg cggacctttt
     4381 cacggatcag cttctggaat tccgggcgga ggggcgtaca gggctcttct aaggcgtaaa
     4441 aattatattt atcgcaaagc tctcctaatt taataccgga agtaacatcg gtacgcccat
     4501 gattttccat gataatatca acattgccgc aggcttcgcg gatcgcaaca atacgttctt
     4561 ctaccatatt ataaaaatca gtagagacaa aaccttcaca gtcacggttt ggaatatctt
     4621 tcttatcccg gtcataaaga gtaaaatcca gctttaccgc atcatatccg tcttccattg
     4681 catatttaca gatatctgcg tattcttttg cggtacctct tggccctaca tcttcattcc
     4741 agccaaactg aagctgtgac gcatagcagc ggatgctgtc atttaccttt ccgcctaata
     4801 attcataaac aggaacattt aatacttttc ccttgatatc cattaaggca atgtcaatgg
     4861 cgctgatagc tgcaaaaaca accgggccgc cgccctggcc ccagaagctt actttaaaca
     4921 ttctctccca cagctcatct gtacgcatag gatcttttcc gatgaccaga cgggagatat
     4981 cctggagcat gccgatcccc gcaggtgctg cgtaatcaaa agcgatcccc gcttcaccgt
     5041 caccatagaa accttcatct gtccataccc ggcagcagat gggacgtctg ctggctctct
     5101 gggcagtagc atccagcatg taaatatcaa cttttgttat cttcataaaa aactccattc
     5161 tgccgcccaa cagcagccca aacttttaat aaactttttc ctgttttttc caattaccca
     5221 aagctttctc aactttatca aacgcctcct ccagatacgc tctggggcag gcgaaattca
     5281 cacggataaa accgggagca gaaaaagact ctccgtcatt aaatttcaca cctgcttctt
     5341 tcataaaaaa gccggcagga tcacttaatc cggtttctct ggcatcgatc caacctaaat
     5401 aagtaccttc actgtggcag atagaaagcc ccgggatccg gctgatccgt tcgtccagcc
     5461 attttttatt ttccctcagg tattctaaca gttcctgccg ccattcttcc ccttttgtaa
     5521 aagctgcctc aagggcaaca aaagaaagag tatcaaaagg cgcatgcatc gttgctgcta
     5581 cttcttcgta tttatgtctt aacttttcat ttgggataat ggcaaatgcc accggaagac
     5641 cgggtatgtt ataggtcttg cttgcagaat gatgaaggat cactctctgt ctggctgctt
     5701 catttaaggt aatcgcagga atatgttctc cttccaggat caggtcactg tgtatctcat
     5761 ctgcaaccac cacaatgtca tgtctggcag caaatgcaaa taactcctca agttcctttc
     5821 cggtaaatac acgacctacc ggattatgag gattacacag cacaaaggtg gtcacatcct
     5881 gtgtaaccgc tttttccatt gcctcaaaat caaaggtata cagcccgttt tcctgtttta
     5941 aaggtacacg gatcaccggc tttcccgctt cctggggcaa tttctggtta atatggggat
     6001 acataggagt tgccaccata atggtgccgc ctaaagctct ggcagcaata ttatttcctg
     6061 tattaactcc atggttaaac aggatccatt ctttttttac atcaaaatca tatcttctct
     6121 tataatagga aataactgcc tgataaacac tttctccggg aaatgtatat ccgaaaaatg
     6181 gatgttccag gcgttttttc agtgcttcta aaacaggctc tgccacagga aagtccatat
     6241 ccgcaaccca cagcgggata taatctcctc cctggccgtc ccatttagag cagtctgtat
     6301 tttttctgtt tatctgtctg ttaaaatcaa ttttcatttg ggggactcct ttcgcagccg
     6361 cttgtttttc ttttggactc cttaaactca caagccccgc gctggtgcgc tccactgcct
     6421 gctgacgcag gcggcttgtt cgttaatggc ttgcgaaaaa caaatgccgc ctcgggcggc
     6481 gcttgttttt ctctgcgctc attatatcgt atacgaccac ttaggcagta cacgctctaa
     6541 gaactgcttt gttctttctt cttttggatt ggaaaagatc tcatccggct ttccttcttc
     6601 cactacaaca ccaccggcca taaagatcac acgattggct gcatcagaag caaagctcat
     6661 ttcatgggtc actatgatca tggtgatccc ttcttttgca atatctttga taacttttaa
     6721 tacttctccc accagctccg gatccagggc agacgtaggt tcgtcaaaaa ggatcacatc
     6781 cggatttaac acaacagctc tggcaattcc cactctctgc tgctgtccgc cggaaagctg
     6841 ggacggataa taatctgcct tatccttaag acctaccttt tccaatgcat caagagcgat
     6901 ctcctttgcc ttttcttttg atacgcctct tgcctgggtc agtccttcca ttacatttcc
     6961 aagggccgtt ttattggcaa acagattata attctggaaa accatggcag tccttaaacg
     7021 gatctcatgg atttctttct tctttgcttt tgctgcgtct actgtaatat ctcctactgt
     7081 gatcgttcct tcatctgctc tttccaggaa attaatgcac cgcagcatgg tggtctttcc
     7141 ggagccgctg gggccgatca ctaccaccac atctccttta tttacagtaa tatcaacacc
     7201 ttttaaaacc tggttctgtc caaatgcttt atgtaaattt ttaatctcga tcatatctta
     7261 cctcctgccg catatttata ccgtttttcc attacataaa acagcttttc cacgcagacg
     7321 cacatagccc agtatacgac agccactgca atatatgcct ccagataacg gaaattcaca
     7381 gctgccgaat tgatggccgc tcctaaaata tcatttacag aaataagata tacaatggat
     7441 gtggacttta aaagaccgat cacattgttg cacagaacag gaagggctgc aggaacagcc
     7501 tgtggaaaga ccacccgaaa caatgtcttt gtcctggtaa gtcctatgga atacgcagct
     7561 tcatactgtc cttttcccac ggaaaggaga gcgccaagaa ctgtttctgt gatctgcacc
     7621 actgcataaa tgcttaacgc agcaatacct atatatattt tattcatagt ctgcagcggc
     7681 tttgcccact catagcttaa cgccaggtaa tcaataggct ttaagatcag gtaattgatc
     7741 ataagcacca gcagtaccgc cggtattcct tttaaaatat tagacgcatt ttttaaaaac
     7801 gaagctgcaa aaggaacatt gaagcggcgg atcaccgcaa agggaacacc aaaaaggaaa
     7861 cctataaaca ggggagcaaa agtcagcagc aaagtaaccg gcatagcttc ccatattttc
     7921 ccgatcactg taaaaacata cgaaaactca aagggcgatg ccattttcct tcctccttgt
     7981 ctgaccatta agccggtgct ccatccaggt aaacaaacgc tccagtaaat aggaaacgat
     8041 gataaatatg atcgctgcat ctacatagcc ttccagaaca cggttggtac gtgttccaat
     8101 agcttttacc tgacccatga tatcaataat tcccagtgta aatgccattg cagttgcctg
     8161 gaaagatccg accacacggg ttccaaatgc aggcgctgct attttaaatg cctgtgggat
     8221 aatgatcctt ttataggtct gaaatgtggt aagtcctaca gaagctgccg cttcaagctg
     8281 gcccttggga acactttcaa tagaagaccg gatgatctcc gacataaatg ccccatcatt
     8341 taaaagatat gtaaccagta caaaaaacag cttactccaa tggttaatat ccactcctaa
     8401 gggtgctaca agaagtggca gtccataata gactacaaat agctgcacca ggatcggggt
     8461 acctcttaca aaagagatat aaagagctgc cagttctctt aacactggta ttttaaagat
     8521 acgtgccgca gccagaataa atcccagaac cagcccccct gcaacgctca ctacta
//
LOCUS       DBMDUA010000117         8563 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3755_length_8563_cov_4.059088, whole genome shotgun sequence.
ACCESSION   DBMDUA010000117 DBMDUA010000000
VERSION     DBMDUA010000117.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8563)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8563)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8563
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3755_length_8563_cov_4.059088"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="ACZPCL_09190"
     CDS             complement(<1..1145)
                     /locus_tag="ACZPCL_09190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1162675.1"
                     /translation="MARSLYSKFVLGYLIFGLLSVLTIATFSSGITRDYLIKDRANAL
                     YDEANDIAASCSLMYQGQHQDMDLFSTQLKNVSTYLHTETWVVNKSGVVIMDSKNGSR
                     VHTTIPDFDPTATGNRSYTIGTYYKLFDQNVLSVSAPVTGNYTTYGYVLIHLPISEIA
                     DSQSHILDILYMTCAIIFGLSLIILVVFTITVYLPLRKIAAGANEYAAGNLNYRIKVK
                     THDEMGYLSNTLNYMSDELNKMEEYQRNFIANVSHDFRSPLTSIKGYLEAILDGTIPP
                     EMYEKYLSRVISETERLHKLTESMLTLNSLDAKGFLSRTNFDINRVIKDTAASFEGTC
                     DSRHIRFDLTFSDNIQMVYADMGKIQQVLYNLIDNAIKFSHDDSVIY"
     gene            complement(1150..1860)
                     /locus_tag="ACZPCL_09195"
     CDS             complement(1150..1860)
                     /locus_tag="ACZPCL_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1162676.1"
                     /translation="MAEKQRILIVDDDANIAELISLYLMKECYETMIVGDGEEALKRF
                     PEFKPNLVLLDLMLPGMDGYQVCRELRSSSQVPIIMLSAKGEIFDKVLGLELGADDYM
                     IKPFDSKELVARVKAVLRRYQASPAAAITEPKQQGDYVEYPDLIVNLTNYSVIYKGHS
                     IEMPPKELELLYFLASSPNQVFTREQLLDHIWGYEYIGDTRTVDVHIKRLREKIKDHA
                     SWAITTVWGIGYKFEVKR"
     gene            complement(1895..4405)
                     /locus_tag="ACZPCL_09200"
     CDS             complement(1895..4405)
                     /locus_tag="ACZPCL_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR1162677.1"
                     /translation="MNQKALKTLEYTKIIAQLESHAASPLGKALCRDLVPSSDLEEVR
                     TWQAQTTDAADRVRLKGTVSFSGLRDIGSSLKRLEIGSALSISELLSISSVLTVAARA
                     KAYGRQDVPENTFTPRFPGQQPPKQTAAEEYVPDSLDPLFQALEPLTPVNNEIKRCIL
                     SEDEIADDASPGLSHVRRSLKACADRIHTQLNSILNSHRTYLQDAVITMRDGRYCLPV
                     KSEYKSQVSGMVHDQSATGSTLFIEPIAIVKLNNEIRELEIQEQKEIEAVLASLSNQT
                     APHIEELQMDMALLAQLDFIFAKAALSHQYRCTAPIFNDKGYINIKDGRHPLLDQKKA
                     VPINVWLGKDFDLLIVTGPNTGGKTVSLKTVGLFTLMGQAGLHIPAWEGSELAVFDNV
                     FADIGDEQSIEQSLSTFSAHMTNTVRILEEADSRSLCLFDELGAGTDPTEGAALAIAM
                     LSFLHNMKCRTMATTHYSELKVFALTTPGVENACCEFNVETLQPTYRLLIGVPGKSNA
                     FAISKKLGLPDYIIEDAKNHLEAKDESFEDLLTSLENSRVTIEKEQEEIRSYKEEIAQ
                     LKSRLTRKEEHLDERKDKVIRNATEEAQRILREAKETADQTIRQINKLAADSGINKEL
                     EAQRTKLREQLKKTDDKLAVKAKGPSQPVSAKKLKIGDGVKVLSMNLKGTVSSLPDST
                     GNLFVQMGILRSKVNIRDIELIREDDISATLGDGSSRSCGAVSGVGISKSKKTFSQAK
                     GSNSGSGQIKMSKSFSVSPEVNLIGMTTDEAVPTMEKYLDDAYLAHLPSVRVVHGRGT
                     GALKTACHKRLKQLKYVKDFRLGEFGEGGTGVTIVTFK"
     gene            4632..5957
                     /locus_tag="ACZPCL_09205"
     CDS             4632..5957
                     /locus_tag="ACZPCL_09205"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR1162678.1"
                     /translation="MADKRTDKDDNVKEERSWQFIQEKIVRQPLSRRQIVRYIILTVI
                     CAVLFGTISAVCFTVTKTVVRKIMGQEKIEESRSITIPKDEPETSAPVQQTQEASTEE
                     TEPVEEKVRSELEKYPYSLEDLNKMYGNLRTLVSEADSSIAAIHSIQHEKDWFDNPIE
                     TTGQFSGVVIDARKEEVLILAPTEAIETADSLEVAFSDSAVLPGAVKQTDSRMGLTMI
                     RVDATSLDDAQFEKIKPVKLGNSYGVRQGDLVISMGSPAGMVHSSSYGFISYIAKNVQ
                     MTDGNARVLYADIKSKADAGTFLFNTEGELVGWATDRYEQDVDTGMKTFMSISDYKGA
                     LELLSNGIQVPYLGIEGQEVTSEMEKNGMPSGIYMIAAAAESPAYNAGLQPGDILSGI
                     NDIKIEGMKNFQAQVEKLHVGDRITVTVQRNNGKDEYKEIEFEVTVGAR"
     gene            6018..6962
                     /locus_tag="ACZPCL_09210"
     CDS             6018..6962
                     /locus_tag="ACZPCL_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HIR1162679.1"
                     /translation="MRYIETFREGMHVSDVYLCKNKQIQLTKNGKEYGNLVLQDKTGT
                     IDAKIWDLNSPGVGDFDTMDYVCIEADVTLFQNSNQLNVRRIRKAGEGEYIESDYLPV
                     SKRDIGKMYEELLAFIKSVKNPYLNRLLSSFFVDDAAFAKAFQFHSAAKTVHHGFVGG
                     LLEHTLSVTKLCDYYAGYYKELNRDLLITAAIFHDIGKTKELSRFPENDYTDDGQLLG
                     HIIIGTEMISDRMKEIEGFPASIAVELKHCILAHHGELEYGSPKKPALLEALALNFAD
                     TTDAKMETMIEALNAGGEIKGWLGFNRFLDSNIRRTTE"
     gene            6972..>8563
                     /gene="rlmD"
                     /locus_tag="ACZPCL_09215"
     CDS             6972..>8563
                     /gene="rlmD"
                     /locus_tag="ACZPCL_09215"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1162680.1"
                     /translation="MEKNQEFTVSIEDMSEDGAGIGKLDGYIWFIKDAVIGDVVRARA
                     MKMKKNYGFARLMEILTPSEARVEPKCPVARQCGGCQLQAMSYEEQLKFKECKVKNHL
                     LRIGKFKEDEIHMLPIMGMKEPWRYRNKAQFPFGLDKNGNIVAGFYAGRTHDIIECED
                     CLLGVEENKDILGIIKGFMRVHGIRPYDETTHKGLVRHALIRKGFHSGEIMVCLVING
                     KDIPAKEQLVEELCQVKGMTSISYSINQEKTNVIMGKEIVNLYGPGYITDNIGNVTYQ
                     ISPLSFYQVNPVQTEKLYGTALEYAGLTGNETVWDLYCGIGTISLFLAQNAKKVYGVE
                     IVPQAIEDARRNATLNNITNAEFFTGKAEEVLPEQYEKNKVYADVIVVDPPRKGCDQV
                     CLDTILKMAPKRVVYVSCDSSTLARDLRYLADGGYEVEKVRCCDMFGQSCHVETVVLL
                     TQQKLDDTIEIDLDLDELDATSAELKATYQEIKDYVLKEFGLKVSSLYISQVKRKCGI
                     EVGENYNLPKSENARVPQCPKE"
BASE COUNT         2385 a         1760 c         2161 g         2257 t
ORIGIN      
        1 atgtagatca cagagtcatc atggctgaac ttgatggcat tgtcgatcag gttataaagg
       61 acctgctgga tcttgcccat gtctgcgtag accatctgga tgttatcgga aaatgtaagg
      121 tcaaagcgga tatgtctgga gtcacaggtt ccttcaaagg atgcggcagt gtcttttatt
      181 acccggttga tatcaaaatt agttctggat aaaaaccctt ttgcatctaa ggagtttaaa
      241 gtcagcatgc tttctgtcag tttatgaagg cgctctgttt cagaaatcac tctggataag
      301 tatttttcat acatttccgg cggaatggtg ccgtccaaaa tagcttccag atatcctttg
      361 atggaggtca gcggtgaacg gaaatcatgg gagacattgg caatgaaatt gcgctggtat
      421 tcttccattt tattcagctc atcggacata taatttaggg tattggaaag gtagcccatt
      481 tcatcgtggg tctttacctt gatccggtag tttaagttgc cagcagcgta ttcattggca
      541 ccggctgcga tctttcgcag aggcagatat accgtgatgg taaatactac aaggatgatc
      601 agcgacaggc caaagataat ggcacaggtc atgtagagaa tgtccaaaat atggctctgg
      661 gaatctgcaa tctcactgat gggaaggtgg atgagcacgt aaccatacgt agtgtaattg
      721 ccggtcacag gagcggaaac actgagtaca ttctggtcaa ataacttgta ataggtgccg
      781 atggtataag aacggtttcc cgttgccgtg gggtcaaaat ccgggatggt ggtatggacg
      841 cggctgccat ttttactgtc catgatcaca acgccgcttt tgttgaccac ccatgtttct
      901 gtgtgcagat aggtggacac attttttaat tgtgtggaaa aaagatccat atcctgatgc
      961 tggccctgat acataagact gcaggaagcc gcaatatcat ttgcctcatc gtaaagggca
     1021 tttgcccggt ccttgatcag ataatcccgg gttataccgg aagaaaaggt ggcaatggta
     1081 agcacgctta aaaggccgaa gatcaggtag cccaggacaa atttggaata aagagagcgg
     1141 gccattacat cacctcttta cttcaaactt gtagccaatg ccccagactg tagtaatggc
     1201 ccagctggcg tgatctttga tcttttcacg cagtcgtttg atgtgaacgt ctactgtacg
     1261 ggtatcacca atgtattcat atccccagat atgatctaac agctgctctc tggtgaagac
     1321 ctggttggga gaggaagcta agaaatataa cagctccagc tcttttggag gcatttcaat
     1381 ggaatgacct ttgtaaatga cggaatagtt ggtcaggttt acaatcaggt ccgggtattc
     1441 cacataatcg ccctgctgtt tcggctctgt gatggcagct gcaggtgaag cctgataacg
     1501 gcgcaggacc gcttttaccc tggctaccag ttccttggag tcaaatggtt tgatcatgta
     1561 gtcatcagca cccagttcca gaccaaggac tttgtcaaag atctcacctt ttgcggaaag
     1621 catgatgatc ggcacctggg aactggagcg cagctctctg catacctgat agccgtccat
     1681 acctggaagc atcaggtcta aaagtaccag atttggttta aattccggga aacgctttaa
     1741 ggcttcttcc ccgtcgccta cgatcatggt ttcataacat tctttcatca gataaagcga
     1801 gatcagctct gcgatgttgg catcatcatc tacaatgagt atccgctgtt tttctgccat
     1861 tacaatttac ctccctaaaa gttagattat gttcctattt gaatgtaaca atagtaacac
     1921 cggttccgcc ttcgccaaat tcgcccagac ggaagtcttt tacatatttt aactgtttta
     1981 aacgcttgtg gcaggcagtc tttaaggcgc cggtacctct tccgtgtacc acgcgtacac
     2041 ttggcaggtg ggccagataa gcgtcatcta agtatttctc catagtagga actgcttcgt
     2101 ctgttgtcat accgatcaga ttgacttccg gtgaaacaga gaaggacttg gacatcttga
     2161 tctgtccgga gccggaatta gagccttttg cctgtgaaaa ggtctttttg gatttgctga
     2221 ttcctacacc ggaaacagca ccgcaggaac gggaagaacc atctcctaag gtagcgctga
     2281 tatcgtcttc acggatcagt tcaatatcac ggatattgac cttggaacgc aggataccca
     2341 tctgtacaaa caggtttccg gtgctgtctg gaagggaact gacagtacct tttaagttca
     2401 tggaaaggac ttttacgccg tcaccgatct ttaacttctt ggcagaaaca ggctggcttg
     2461 gacctttggc ctttactgcc agtttgtcgt ctgttttctt taactgctca cgcagtttgg
     2521 tcctctgggc ctctaattct ttgttaatgc cggagtcagc agccagtttg ttgatctggc
     2581 ggatggtctg gtcggcagtc tcttttgctt cacgaaggat gcgctgtgcc tcttctgttg
     2641 cattgcggat caccttgtcc ttgcgctcat ccagatgctc ttccttgcgg gtgaggcggg
     2701 attttaactg ggcgatctcc tctttatagg aacggatctc ttcctgttct ttttcaatgg
     2761 tcacacggct gttttccagg ctggtaagca ggtcctcaaa ggactcatct tttgcttcca
     2821 gatggttctt tgcatcttca ataatgtagt ccggcagtcc aagttttttg gaaatagcaa
     2881 acgcattact ctttcccgga acaccgatca gcaggcggta ggtcggctgc agtgtttcta
     2941 cgttaaactc gcagcatgca ttttccacgc ccggggtagt aagtgcaaat acctttaatt
     3001 cgctgtaatg ggtggttgcc atggtacggc atttcatatt gtgtaaaaat gacagcatgg
     3061 caatggcaag ggcagcacct tctgttgggt cggtaccggc tcccagctca tcgaaaaggc
     3121 agagggaacg ggagtctgct tcctctaaaa tacgtaccgt attggtcatg tgggcggaga
     3181 aagtacttaa actctgctca atgctctgct catcaccgat atcagcaaat acattgtcga
     3241 atacagctaa ttcagaacct tcccaggccg gaatgtgaag tcccgcctgt cccattaagg
     3301 tgaaaagacc tacggttttt aaggaaacgg tcttaccgcc ggtattagga cctgttacga
     3361 tcagcaggtc aaagtctttt ccaagccata cattgatagg tactgctttt ttctggtcca
     3421 gcaatgggtg gcgtccgtcc ttgatattga tgtagccctt atcattgaaa attggggctg
     3481 tgcagcggta ctggtgggaa agtgcagctt tcgcaaaaat gaaatccagc tgtgctaaca
     3541 gtgccatgtc catctgcagt tcttcaatat gaggagctgt ctggttgctt aaagaagcta
     3601 aaacagcctc gatctctttc tgctcctgga tctccagttc gcggatctca ttgtttaatt
     3661 ttacaatggc tattggttca ataaaaaggg tagaacctgt ggcagactgg tcatggacca
     3721 tgccggatac ttggcttttg tactctgatt taacagggag acagtaccgg ccgtcacgca
     3781 tggtgatcac agcgtcctgt aaataggtac ggtgggagtt caggatagag ttcaactggg
     3841 tatggatacg gtctgcgcag gcttttaagg aacggcgcac atgggacagg ccgggacttg
     3901 catcatcagc gatctcatct tcggaaagga tgcagcgctt gatctcatta ttcactggtg
     3961 taagcggctc taaggcctgg aataacgggt ctaaggagtc aggcacatat tcctcggctg
     4021 ctgtctgttt cggtggctgc tgacctggaa aacgtggtgt aaaggtgttt tccgggacgt
     4081 cctgacggcc gtaggctttt gcacgggcag caactgtaag aacagagctg atagaaagca
     4141 gttcgctgat gcttaaagca ctgccgatct ccaggcgctt taaagaactg ccgatgtccc
     4201 gcaggccgga gaaggagaca gtgcctttta agcggactct gtcagccgcg tcggtggtct
     4261 gcgcctgcca ggtacgaact tcttccaggt cagaagatgg cacaagatcc cggcagaggg
     4321 ctttacccag tggggaagcc gcatgggact ccagctgggc gatgatcttt gtatattcta
     4381 aagtttttaa ggctttttgg ttcataaatg ttggttcctc cgagtgatag gaaaataagc
     4441 gcttgttttt tctttgcgcc tttacattat accagtaaac attctgtttt actacgggaa
     4501 atttctcagg tggtgcgggg cgaaaaccca gtaagacggg cacttttggc tcaagaatcc
     4561 catagaacat cttgcataat agcaggaaga accttataat aggaataaga ttcttcgcaa
     4621 ggaggaacgc aatggcggat aagcgtacag ataaggatga caacgtaaaa gaagagcgtt
     4681 cctggcagtt tatccaggag aagatcgtgc gtcagccgtt atcaagaagg cagatcgtca
     4741 gatatattat attgactgtt atttgtgcag tactgtttgg gaccatatcg gcggtttgtt
     4801 ttacagttac gaaaacggta gtcagaaaga taatggggca ggaaaaaata gaagaaagcc
     4861 gttccattac aataccgaaa gatgaaccgg aaacatcagc tccggtacag cagactcagg
     4921 aagcttcaac agaagagaca gaaccggtag aagagaaagt tcgttcagag ctggaaaaat
     4981 atccatattc tctggaagat ctgaacaaga tgtacggaaa tttaaggaca ctggtatcag
     5041 aagcagatag ttccattgct gcgatccatt ccatccagca tgaaaaagac tggtttgata
     5101 acccgatcga aaccacagga cagttttcag gtgttgtgat cgatgccaga aaagaagaag
     5161 ttctgatcct ggcaccaaca gaagcaatag agacagcaga ctctctggaa gttgcatttt
     5221 cagacagtgc cgttcttcct ggagctgtaa aacagacaga ctcccggatg ggactgacca
     5281 tgatccgtgt agatgctact tcactggatg atgcacagtt tgagaagata aaaccggtaa
     5341 aactgggaaa ttcctatggg gtcagacagg gagatctggt tatatccatg ggttcaccgg
     5401 caggtatggt acactccagc agctatggtt tcatttccta tatagctaaa aatgtacaga
     5461 tgacagatgg aaatgcccgt gttctgtatg cggatatcaa aagtaaggca gatgcaggga
     5521 ctttcctgtt taatacagag ggagaactgg ttggctgggc cacagaccgc tatgagcagg
     5581 atgtggatac aggcatgaaa acatttatgt ccatttctga ttataaagga gctctggaac
     5641 ttttaagtaa tgggatccag gtgccttatt taggcattga aggtcaggaa gttacttcag
     5701 aaatggaaaa aaatggaatg ccatctggta tttatatgat cgcggcagct gccgaaagtc
     5761 ctgcctataa tgccggactt cagcctggtg atattttgtc cgggatcaat gatattaaga
     5821 tcgagggaat gaagaatttc caggcccagg tggaaaaact ccatgtagga gaccgcatta
     5881 cagtaacagt ccagagaaac aatggaaaag atgagtacaa agagattgaa tttgaagtaa
     5941 cagtgggagc caggtaggaa aggtgccggg ctctctttta tggaaaaaat caaaattaga
     6001 aaagatgagg taagaaaatg agatatatcg aaaccttccg tgaaggtatg cacgtatctg
     6061 atgtgtattt atgtaaaaat aagcagatcc agcttacaaa aaatggaaaa gaatatggca
     6121 acctggtcct tcaggacaag acaggaacca ttgatgccaa gatctgggac ttaaattctc
     6181 ctggtgttgg agactttgat accatggatt atgtttgcat tgaagcagat gtgaccttat
     6241 tccagaactc caaccagtta aatgtacgcc gcatccgcaa ggcaggagaa ggagagtata
     6301 tagagtctga ttatctgcct gtatccaagc gggatatcgg aaaaatgtat gaagagcttc
     6361 tggcatttat caagagcgta aagaacccgt acttaaaccg cctgctgtcc agcttttttg
     6421 tagatgacgc tgcttttgca aaagcattcc agttccattc tgcagcaaag accgttcatc
     6481 atggttttgt aggcggactg ttagagcata cgttaagtgt tacaaagctg tgtgattatt
     6541 acgcaggata ttataaagag ttaaaccgtg atctgctgat cacagcggct attttccatg
     6601 acatcggcaa gacaaaagaa ctgtcccgtt tcccggaaaa tgattatacc gatgacggac
     6661 agttactggg ccatatcatc attggcacgg aaatgatatc agaccgtatg aaggagatcg
     6721 aaggctttcc ggcaagtatt gcagttgagt taaaacactg tatcctggca catcacgggg
     6781 agctggaata cggttctcct aagaagccgg ccttacttga ggcactggca ctgaattttg
     6841 cagatacgac agatgcaaag atggagacca tgatcgaggc attaaatgct ggtggcgaaa
     6901 tcaagggatg gttgggattt aaccgtttct tagactctaa tatccgcaga acaacggagt
     6961 aaaatagaaa tatggaaaaa aatcaggaat ttacagtatc cattgaagat atgagtgaag
     7021 acggcgcagg gatcggtaaa ctggatggtt atatctggtt catcaaagat gcagtgatcg
     7081 gagatgtagt ccgtgcccgt gccatgaaaa tgaagaaaaa ttacgggttt gcaaggttga
     7141 tggagatcct tacgccgtca gaggcaagag tagagccaaa gtgcccggtt gcccgccagt
     7201 gcggcggctg ccagcttcag gctatgtcct atgaagagca gctgaaattt aaagagtgta
     7261 aggtaaaaaa ccacctgtta aggatcggaa agtttaagga agatgagatc cacatgcttc
     7321 ccatcatggg aatgaaagag ccgtggcgtt accgcaacaa agcacagttt ccgtttgggt
     7381 tggataaaaa tggaaatatt gtggctggat tttatgcagg ccgtacccat gacattattg
     7441 agtgtgaaga ctgtctgtta ggtgtggaag aaaataagga tattttaggc attatcaaag
     7501 gatttatgag agtccatggg atccgccctt atgatgagac gacccataag ggcttagtgc
     7561 gtcatgcact gatccgcaaa ggcttccatt ccggtgagat catggtctgt ctggtgatca
     7621 acggaaaaga tattccggca aaggaacagc tggtagaaga actgtgccag gtgaaaggca
     7681 tgaccagcat ttcctacagc atcaatcagg aaaagaccaa tgtgatcatg ggaaaagaga
     7741 tcgtaaattt atatggccct ggttacatca ctgataatat tggaaatgtg acctatcaga
     7801 tctctccgtt gtccttttat caggtcaatc cggtgcagac ggaaaaattg tatggaaccg
     7861 ccctggaata tgcgggactg accggaaatg aaacagtctg ggatctgtac tgcggaatag
     7921 gaaccatttc cctgttttta gcccagaatg caaagaaggt ctacggtgtt gagattgttc
     7981 cccaggccat tgaagatgcc agaaggaatg ctaccttaaa taacattacc aatgcagaat
     8041 tcttcaccgg aaaggcagag gaagtccttc cggaacagta tgaaaagaac aaagtctacg
     8101 ccgatgtcat cgtagtagac cccccaagaa aaggctgcga ccaggtctgc ctggatacca
     8161 ttttaaaaat ggcaccgaaa cgggtggttt atgtcagctg cgactcctct acgctggcaa
     8221 gagatttaag atatcttgca gatggcgggt atgaggtaga gaaggtaaga tgctgcgata
     8281 tgtttgggca gagttgtcat gttgagacgg ttgttctttt gacccaacag aagctggatg
     8341 acacgataga gatcgactta gacctggacg agctggatgc caccagtgcc gagttgaaag
     8401 caacctatca ggaaatcaaa gattatgtgt tgaaagaatt tggcttgaag gtttcaagtt
     8461 tatatatttc tcaggtaaaa cgcaaatgtg gaattgaagt gggagaaaac tataatcttc
     8521 caaaatcaga aaatgcaaga gttccacaat gcccgaaaga gaa
//
LOCUS       DBMDUA010000118         8536 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3767_length_8536_cov_1.959573, whole genome shotgun sequence.
ACCESSION   DBMDUA010000118 DBMDUA010000000
VERSION     DBMDUA010000118.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8536)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8536
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3767_length_8536_cov_1.959573"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /gene="ftsY"
                     /locus_tag="ACZPCL_09220"
     CDS             <1..858
                     /gene="ftsY"
                     /locus_tag="ACZPCL_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR1162681.1"
                     /translation="GIDSIFSGFSAIDDDFYEEIEETLIMGDLGIQTTMAIVEDLRNK
                     VKEQHIKDPEQCKEILIGSIKEQMDLGENAYEFENRKSVVLVIGVNGVGKTTSVGKLA
                     GQLKDQGKKVILAAADTFRAAAIEQLTEWSARAGVDIIAQQEGSDPAAVIYDAVAAAK
                     SRKADVLICDTAGRLHNKKNLMEELKKINRIIDKEYPDAYRETLVVLDGTTGQNALAQ
                     AKQFMEVADITGIILTKLDGTAKGGIAVAIQSELGIPVKYIGIGEKIDDLQKFDANDF
                     VNALFHVQS"
     gene            944..2176
                     /gene="ilvA"
                     /locus_tag="ACZPCL_09225"
     CDS             944..2176
                     /gene="ilvA"
                     /locus_tag="ACZPCL_09225"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR1162682.1"
                     /translation="MEELTLEKFEEASEIVKEVTLETKLIYSEYFSAQTGNKVYFKPE
                     NMQYTGAYKVRGAYYKISTLTEEEKAKGLITASAGNHAQGVAYAAKLSGVKATVVMPT
                     TTPLIKVNRTKGYGAEVVLAGDVFDEACAYAYKLADEHGYTFVHPFDDLAVATGQGSI
                     AMEIIKELPTVDYILVPIGGGGLCTGVSTLAKLLNPKIKVIGVEPAGANCMQESLKEG
                     HVLTLPQVNTIADGTAVKRPGEKLFPYIQQNVDDIITIEDSELIVAFLDMVENHKMIV
                     ENSGLLTVAALKHLNVEKKKIVSILSGGNMDVITMSSIVQHGLIQRDRVFTVSVLLPD
                     KPGELAKVAELLAKEHGNIIKLEHNQFISINRNAAVELRITMEAFGTDHKNQIVSALT
                     DAGYRPKLVKFKGTYSEM"
     gene            2296..3558
                     /locus_tag="ACZPCL_09230"
     CDS             2296..3558
                     /locus_tag="ACZPCL_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR1162683.1"
                     /translation="MNHSADETRASLANNLRYFIKWSLIALLIGGTGGLIGSLFSMGV
                     SFATSFRMNHPNMLFFLPVSGILIVWLYHTFHEEGNRGTNMVIDAISSTEKVTLATGP
                     LIFVSTVLTHIAGGSSGREGAALQLGGSLGNLFGNAIHLDEKDKKIAIMCGMSAVFSA
                     LFGTPVAAAIFPLEVISIGVLYYAALVPCIFSSFIGVGIARYLGIAGEHFTVLSIPAI
                     SVSSVGWIIFLSALCAGVSIVFCLMLHGAEHTYRKIFPNPYVRILAASAIFILLTLLI
                     GTRDYCGSSMPLIERSMEGEIGYEAFFLKMLFTAIALGAGFKGGEIVPTLCVGAAFGC
                     AFGQITGFAPSLCAACSMAALFAGVTNCPISSLVIALELFGYEGMEYFSIAIAVAFAL
                     SGYYGLYASQKFVYSKTKTEFINRKSNK"
     gene            3608..4159
                     /locus_tag="ACZPCL_09235"
     CDS             3608..4159
                     /locus_tag="ACZPCL_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HIR1162684.1"
                     /translation="MKKRTYLISLSLAAGLALSQLTGCASSGPEETTAAESTQAPSEA
                     ETASIPNPMEEVENEQSFESMGIHMVAPADGENTKFYTISGEVAEITFDENGVSYCYR
                     ASNTAEDFAGIFERFTDQELAVNWESGKIAGQAQVKTTESGGRLASWSWGSTRYTVYT
                     ASAISDEECTQVVTNLMELSYHE"
     gene            4199..4492
                     /locus_tag="ACZPCL_09240"
     CDS             4199..4492
                     /locus_tag="ACZPCL_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162685.1"
                     /translation="MLQLKDFMPVNFLKKEKYTGSCKGMRFRMEKFEKEGEETPVLRV
                     SVWPEPYSFDCTPEEEKQFLECTFDADGIAKGADWINERYEAELPRWKKVSRL"
     gene            4750..6462
                     /locus_tag="ACZPCL_09245"
     CDS             4750..6462
                     /locus_tag="ACZPCL_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR1162686.1"
                     /translation="MEFSAVNTIWVLVGAALVFFMQAGFAMVETGFTRAKNAGNIIMK
                     NLMDFAIGTPLYWLTGFGIMFAGSGAFIGGFDPFVRGDYSAILPAGVPLPAYLIFQTV
                     FCATAATIVSGAMAERTKFISYCIYSAVISAIVYPISGHWIWGGGWLAQLGFHDFAGS
                     TAVHMCGGVAALIGAKILGPRIGKYDKDGKPKAILGHSLTLGALGVFILWFCWFGFNG
                     CSTVAMDSDEAIYSAGNIFVTTNLAAAVATVAVMIITWIRYKKPDISMTLNGSLAGLV
                     AVTAGCDVVTPTGAFFIGLIAAFVVVFGIEFVDQVLKIDDPVGAVGVHCFCGAVGTLL
                     TGLFDKENGVFYGDGFHMLGIQLLGVVSVILWVTITMVIVFNLLKHTIGLRASVEEET
                     KGLDVTEHNLASSYADFMPVVFTGREDAPAVSAAPVSVEKSVPVEHFPQSETTISGNG
                     SGAKLTKVVMIFNQSRFVALKEALTELGVTGMTITQVMGCGTQKGHTNYYRGIKVEEA
                     ALLPKIKLEVVVSKVPVADVIALAKKVLYTGHIGDGKIFVYDVENVVKVRTGEEGYDA
                     LQGD"
     assembly_gap    6640..6649
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6650..7501
                     /locus_tag="ACZPCL_09250"
     CDS             <6650..7501
                     /locus_tag="ACZPCL_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIR1162687.1"
                     /translation="DSVPGVSGGTIAFLLGFYDEFINSLNDMIGKDNAKRKAALVFLI
                     KLGIGWVIGFGASVMVLSSLFQVHIYEISSVFLGLTLFAIPLVVKEEWASIKGKAGCL
                     VFTLIGMALVFCISYFNPTNGNGISVNFEHMTAGLGLYLFFAAMIAITAMVLPGISGS
                     TLLLIFGLYVPVINAIHEFMHLNLSYLPVLVIFGLGVLTGIVSIIKIIKICLAKHRSQ
                     TIYMILGLMIGSLYAITQGPTTLDVPQQPMSFSTFHIGFFILGAVILAGLELLKTRLE
                     KQDGGRK"
     gene            7501..>8536
                     /locus_tag="ACZPCL_09255"
     CDS             7501..>8536
                     /locus_tag="ACZPCL_09255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1162688.1"
                     /translation="MLKQLKRKGILALACVISIFSMSVCAFAKPDWPLDTGCQSEAGI
                     VMDLDSGAVLFAQNIHVQEYPASITKLLTALVVVENASMDEQVTFSHDAVYNVESGSG
                     NKLQLEEGDVLSVKDCLYVMLLQSSNQAANALAEHVGGSREAFADMMNEKAASLGCRE
                     SHFVNPSGLNDPEQLTSAYDMAQIGAAVFGNPTLLEICSTTSATLPPTINNPNGRTYS
                     MEHKLVVTGDSSDENYYPSAVAGKTGYTSLAGQTLVTYAEQDGRRQVAVTLKSTQRTH
                     YSDTKTILDFGFARFKNVSVAENETDYVTGEEPVTIGDETYSPSDLYLDEKAVVTLPN
                     DAQFSDADKYL"
BASE COUNT         2314 a         1773 c         2044 g         2395 t
ORIGIN      
        1 ggaattgact ctattttcag cggtttttcc gctattgatg acgatttcta tgaagagatc
       61 gaagaaaccc tgatcatggg cgatttgggt atccagacga ccatggcgat tgtagaagat
      121 ctgcgaaata aagtaaaaga gcagcatatc aaggatccgg agcagtgcaa ggagatcctg
      181 ataggtagca tcaaagaaca gatggatctg ggtgaaaatg cttacgaatt tgaaaacaga
      241 aagtccgttg tcctggtgat cggtgtcaat ggcgttggaa agactacttc tgtaggaaaa
      301 ctggcaggcc agttaaagga ccagggcaag aaagtgattt tagcagcagc agataccttc
      361 cgtgcagcag caattgagca gcttacagaa tggtctgcca gagcaggagt tgatattatc
      421 gcacagcagg aaggttctga cccggcagct gtgatctatg atgctgtagc agcagccaaa
      481 tccagaaagg cggatgtact gatctgtgat acagcaggcc gtcttcacaa caagaagaac
      541 ctgatggagg agttaaagaa gatcaaccgc atcattgata aggaatatcc ggatgcatac
      601 cgggagactt tagttgtttt agacggaaca acaggacaga atgcgctggc gcaggcaaaa
      661 cagtttatgg aagtagctga tattacaggt atcatcctta ccaagcttga cggtactgca
      721 aagggcggta ttgcagttgc catccagtct gaactgggaa ttcctgtaaa atatatcggt
      781 atcggtgaaa agatcgatga cctgcagaaa tttgacgcaa atgatttcgt caacgccctg
      841 tttcatgtac aatcttaaat atcatggctg aactgtaact tgaatttaat gagataaagt
      901 gttatagtta tacatgataa tcaattttag gagaaagact accatggaag aactcacatt
      961 agagaaattt gaagaggcct ctgagatcgt aaaagaggtc actctggaga caaagctgat
     1021 ctacagcgaa tatttttccg cacagaccgg aaataaggta tatttcaagc cggagaacat
     1081 gcagtatacc ggcgcttaca aggtaagagg tgcttattac aagatcagca ctctgactga
     1141 agaagaaaaa gcaaagggcc tgatcactgc ttctgcagga aaccatgcac agggcgttgc
     1201 ctatgcagca aagctgtctg gtgttaaggc aaccgttgtt atgcctacta caactcctct
     1261 gatcaaggta aaccgcacca agggatacgg agcagaagtt gttctggctg gtgatgtatt
     1321 tgatgaagcc tgtgcatatg catacaagct ggcagatgaa catggctata ctttcgtaca
     1381 tccatttgat gacctggcag tagctaccgg acagggctct attgctatgg agatcatcaa
     1441 ggaacttcct acggtagatt atattttagt tccaatcggc ggcggcggtc tttgtaccgg
     1501 tgtttccact ctggcaaaac tgttaaatcc aaagatcaaa gtcatcggtg tagagcctgc
     1561 tggcgctaac tgcatgcagg agtctttaaa agagggacat gtattaactc ttcctcaggt
     1621 aaataccatt gcagacggta ctgctgtaaa gcgtcctggc gaaaagctgt tcccatacat
     1681 ccagcagaat gtagatgaca tcatcactat tgaagacagc gaactgatcg ttgctttcct
     1741 ggatatggta gagaaccaca agatgatcgt tgaaaactca ggccttctta cagtagctgc
     1801 cttaaagcac ttaaatgttg aaaagaagaa gatcgtttct atcttaagcg gcggaaatat
     1861 ggatgtgatc accatgtcct ctatcgttca gcacggtctg atccagagag acagagtctt
     1921 cactgtatct gttctccttc ctgacaaacc aggtgaactt gcaaaggtag cagagcttct
     1981 tgcaaaagaa cacggcaata tcatcaagct ggaacacaac cagtttatca gcatcaaccg
     2041 taatgctgct gttgagctgc gtatcactat ggaggcattt ggtaccgacc acaagaacca
     2101 gatcgtttct gctcttacag acgcaggcta tcgtccaaaa cttgtaaaat ttaaaggtac
     2161 ttacagcgaa atgtaagcct tcgtgtgcat aaaaagcgtg tctgatggac gaaaaagaag
     2221 tcaggcacgc ttatgtcatt tatagtgatg ccatgatcag atggcatgta agaataatta
     2281 gggggataca tacaaatgaa tcattcagct gatgaaacaa gggcttccct tgccaataat
     2341 ctgcgttatt ttataaaatg gagcctgatc gcactgctga tcggcggtac aggcggactg
     2401 atcggcagcc tcttttctat gggtgtcagt tttgccactt cttttcgcat gaaccaccca
     2461 aatatgttat ttttccttcc tgtttccggt attctcattg tatggcttta ccataccttt
     2521 catgaagagg gcaacagagg caccaacatg gtcattgatg ccatttcttc cacggaaaaa
     2581 gtaacactgg ctacaggacc gctgatcttt gtctctacgg tgctgaccca cattgcaggc
     2641 ggttcttccg gccgcgaggg agctgccctc cagttaggcg gaagccttgg aaacctgttt
     2701 ggaaatgcca tccatttaga cgaaaaagat aaaaagatcg ccattatgtg cggtatgagc
     2761 gctgtgttct ctgcactttt tggaacaccg gtagcagcag ctatcttccc gctggaagta
     2821 atcagtatcg gtgtactgta ttatgcagcc ctggttccct gtattttttc ttcttttatc
     2881 ggagtgggga tcgccagata tttaggcatt gcaggagagc attttaccgt gctttctatt
     2941 ccggctatca gcgtttccag tgttggttgg atcatttttt taagtgccct ctgcgcaggt
     3001 gtcagcattg tattctgcct gatgctccat ggtgcagagc acacttacag gaaaatattt
     3061 ccaaatccct atgtgcgtat cctggcagcc agtgctattt ttatccttct taccctgctg
     3121 atcggtacta gggactactg cggaagcagt atgcctctta ttgagcgcag tatggaaggg
     3181 gagatcggtt atgaggcttt cttcttaaaa atgctgttta cagcgattgc ccttggtgca
     3241 ggctttaagg gcggtgagat cgttcctact ttatgtgtag gtgcagcttt tggctgtgct
     3301 tttggacaga tcaccggttt tgccccttct ttatgtgctg cctgctctat ggctgcctta
     3361 tttgcgggag tgaccaactg cccgatttct tctctggtga tcgctttgga gctgtttggt
     3421 tatgagggaa tggaatattt ctccattgcc attgcagtag cttttgcttt atccggctat
     3481 tatggcctgt atgccagcca gaaatttgtt tattctaaga caaaaacaga gtttatcaac
     3541 cgtaaatcta ataaataaca agttacagtt cagaaatgaa cgacaataat aacaggagat
     3601 gatccaggtg aaaaaaagaa cgtatctgat ttccttaagt cttgcagcag gtctggctct
     3661 tagccagctt actggctgtg cttcttcagg accggaagaa accacagcag cagaaagtac
     3721 gcaggctcct tctgaggcag agactgcatc tattcccaat ccaatggaag aagtggaaaa
     3781 tgagcagtcc tttgagtcca tggggatcca tatggtagca cctgcagatg gggaaaatac
     3841 taagttttat accataagcg gggaagtggc ggagatcaca tttgatgaaa acggcgtttc
     3901 ctactgttac cgcgcttcca atacagcgga agattttgca ggtatttttg agcggtttac
     3961 agaccaggaa ctggctgtaa actgggagtc tggcaaaata gcaggacagg cccaggtaaa
     4021 gaccacagaa agcggcggaa gactggcttc atggtcctgg ggcagcacac gttataccgt
     4081 atacactgcc tctgccattt ccgacgaaga gtgtacccag gtggttacca atttaatgga
     4141 attaagttat cacgaataat ggatctggga taaaaacaga tctgcaggag agtgttttat
     4201 gcttcagctt aaagatttta tgccggtaaa ttttttaaaa aaagaaaaat ataccggcag
     4261 ctgcaaaggc atgcgttttc gcatggaaaa gtttgaaaaa gaaggagagg aaactcctgt
     4321 cctcagagtc agcgtctggc cggaaccgta cagctttgac tgcacgccag aggaagaaaa
     4381 gcaattttta gagtgtacct ttgatgcaga tggaattgca aaaggggcag actggatcaa
     4441 tgaacggtat gaggcggaac ttcccagatg gaagaaagtc agccgtctgt aaaaataaca
     4501 aaggcaaggg aaagtttttg aaaaatttct gccttattaa caaaaattta attttctgtt
     4561 gacattctct ttttaggcca ctataatgag ccacatagaa agcgccggtg cttaataccc
     4621 ggtgcttttt ctgtttttac gtaagaacag tcagcacaaa gaggtgcatt gtcaacgtca
     4681 ggtaccggcg ggcaatgcgc ctttttgcat tctatgtgca tttatggaaa gtatgaggag
     4741 gaaactatta tggaattttc agcagtgaac acaatatggg tccttgtagg cgcggcactg
     4801 gtatttttca tgcaggcagg ttttgccatg gttgagaccg gttttacaag ggcgaaaaac
     4861 gcaggcaaca tcattatgaa gaaccttatg gattttgcca ttggaactcc cctttactgg
     4921 ctgactggtt ttggtatcat gtttgccggc agcggtgctt tcatcggcgg ctttgatcct
     4981 tttgtaagag gcgactacag tgccatcctt ccggcaggtg ttcctcttcc tgcctatctg
     5041 atcttccaga ctgtattctg tgccacagca gccaccattg tttccggtgc catggcagag
     5101 cgtaccaaat ttatttctta ctgtatctac agtgcagtga tcagcgcaat cgtatatcct
     5161 atttccggtc actggatctg gggcggcggc tggctggctc agttaggttt ccatgatttt
     5221 gcaggttcca cagcggttca tatgtgcggc ggtgtagcag ccctcattgg agcaaagatc
     5281 ttaggaccga gaattggaaa gtatgacaaa gacggaaagc cgaaagcaat ccttggtcac
     5341 agcttaacac ttggcgcttt aggtgtattt atcctctggt tctgctggtt tggttttaac
     5401 ggctgttcta cggtagctat ggacagtgat gaagccatct attccgcagg aaacattttc
     5461 gtaacaacca acctggcagc agcagtagca accgtagcag tgatgatcat tacctggatc
     5521 cgctataaaa agccggatat ctccatgaca ttaaacggtt ccttagccgg tctggtagca
     5581 gttacagctg gctgtgacgt agtaactcca acaggtgctt tctttatcgg cctgatcgca
     5641 gcttttgtag tagtttttgg tattgaattt gtagatcagg tattaaagat cgatgatcct
     5701 gtcggtgctg ttggtgttca ttgcttctgc ggtgctgtag gaaccctttt aaccggtctt
     5761 ttcgacaaag aaaacggtgt attttacggc gacggcttcc atatgcttgg tatccagctt
     5821 ttaggtgttg tatctgtgat cctctgggta accatcacca tggtgatcgt atttaatctg
     5881 ttaaagcata ccattggact tcgcgcaagt gtagaagagg aaacaaaggg tcttgacgta
     5941 acagaacata acctggcaag ttcttatgca gactttatgc cggttgtatt tacaggaaga
     6001 gaagacgcgc cagctgtttc ggctgcacct gtatctgtag aaaaatctgt tcctgtagaa
     6061 catttcccac agtctgaaac aacgatttcc ggaaatggct ccggcgcaaa acttacaaag
     6121 gtagttatga tcttcaacca gagccgtttt gtagccttaa aagaagccct gacagaactt
     6181 ggggtaacag gtatgacgat cacacaggtt atgggctgtg gaacccagaa gggccatacc
     6241 aattattaca gaggtattaa agtagaagaa gcagccctcc ttccaaagat caagttagaa
     6301 gttgtagtaa gcaaagtccc tgtggcagat gtgatcgccc ttgcgaaaaa agttctttat
     6361 accggccaca tcggcgatgg aaagatcttt gtctatgatg tggaaaatgt agtaaaagtc
     6421 cgcaccggtg aagaaggata tgacgccctt cagggagact gataaaattc cataaagtac
     6481 attttacttg aatttcctcc tgtttcatgt ataatggttc acagaaagct atccttaaga
     6541 cggtatacta agctttttta accagagtga tttcaggagg aaataattat atgtttttaa
     6601 acgcagtacg aggtttctgc atggcattag cggacagtgn nnnnnnnnng acagtgttcc
     6661 cggtgtttcc ggcggcacca tcgctttttt acttggtttt tatgatgaat ttatcaattc
     6721 tttaaatgat atgatcggaa aggacaatgc aaagcgcaaa gcagcccttg ttttccttat
     6781 taaactggga attggatggg tcattggttt tggtgcttct gttatggtcc tttcttctct
     6841 gtttcaggta cacatctatg agatcagttc tgtcttttta ggccttaccc tgtttgctat
     6901 cccattggta gtaaaagaag agtgggccag tataaaagga aaggctggct gccttgtttt
     6961 taccctgatc ggtatggcac tggtattctg catctcttat tttaacccta caaatggaaa
     7021 tggtatttct gtaaactttg agcatatgac agcaggtctg ggcctgtatc tgttttttgc
     7081 tgccatgatc gccattacag cgatggtact tccgggaatt tccggttcca cactgctttt
     7141 gatcttcgga ctttatgtgc cggtgatcaa cgcgatccac gaatttatgc atttaaacct
     7201 gtcttatctt ccggtacttg ttattttcgg actgggagtt cttacgggta ttgtcagcat
     7261 tatcaagatc attaagatct gcctggcaaa gcaccgttcc cagactattt atatgatcct
     7321 tggtttaatg atcggttccc tatatgccat cacccaggga cccacaactc tggatgtgcc
     7381 tcagcaacct atgagttttt ctacgttcca tattggattt tttatcctgg gtgctgtgat
     7441 cttagcagga ctggaacttt taaaaacacg ccttgaaaag caggatggag gaagaaaata
     7501 atgttaaaac agctgaaacg aaaaggtata ctggcacttg cctgtgttat cagtatcttt
     7561 tccatgtctg tgtgtgcgtt tgcaaaaccg gactggccac ttgataccgg ctgccagtca
     7621 gaagcaggta ttgttatgga tctggactcc ggagccgttc tttttgccca gaatatccat
     7681 gttcaggaat atcctgccag cattaccaag cttttgactg ctttggtagt agtggaaaat
     7741 gcctctatgg atgagcaggt taccttttca catgatgctg tttataacgt ggagtccgga
     7801 agcggcaaca aactgcagtt agaagaggga gatgtgctct ctgtaaaaga ctgcctttat
     7861 gtcatgcttc ttcagtcttc caatcaggca gccaacgctc tggcagaaca tgtaggcgga
     7921 agcagggaag cttttgcgga tatgatgaat gaaaaagcgg cttctttagg ctgccgtgaa
     7981 agccattttg taaacccgtc cggtttaaat gatccggaac agcttacatc tgcctatgat
     8041 atggcccaga tcggtgcagc tgtttttggc aatcctactt tactggagat ctgttctacc
     8101 accagcgcta cactgcctcc gaccatcaac aatccaaatg gccgtactta ttccatggag
     8161 cataagttag tagtaaccgg ggactccagt gacgaaaatt attatccaag cgcagttgca
     8221 ggaaaaacgg gttatacttc cctggcaggt cagacccttg ttacttatgc agagcaggac
     8281 ggacgccgtc aggtggctgt taccttaaag agtacccagc ggacccatta ttctgacaca
     8341 aagaccattc tggattttgg ttttgcccgc tttaaaaatg tatctgttgc agaaaatgaa
     8401 acagactatg taacagggga agaacctgtg accattggag atgagactta ttctccttcc
     8461 gatctttatt tagatgaaaa ggcagttgta accctgccaa atgatgccca gttttcagat
     8521 gcagacaaat atcttc
//
LOCUS       DBMDUA010000119         8516 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3775_length_8516_cov_2.535829, whole genome shotgun sequence.
ACCESSION   DBMDUA010000119 DBMDUA010000000
VERSION     DBMDUA010000119.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8516)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8516
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3775_length_8516_cov_2.535829"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="ACZPCL_09260"
     CDS             complement(<1..148)
                     /locus_tag="ACZPCL_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR1162689.1"
                     /translation="MADKKHILTYAGLKQYEDELQNLKVVRRKEVAQKIKEAREQGDL
                     SENAE"
     gene            complement(427..1155)
                     /locus_tag="ACZPCL_09265"
     CDS             complement(427..1155)
                     /locus_tag="ACZPCL_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1162690.1"
                     /translation="MSGHSKFANIKHKKERNDAAKGKIFTVIGRELAVAVKEGGPDPA
                     NNSKLRDVIAKAKANNMPNDTIDRGIKKAAGDAASVNYETLTYEGYGPSGVAIIVDTL
                     TDNKNRTAANVRSAFTKGGGNVGTPGSVSYMFDKKGQIIIDKEECEMDADELMMAALD
                     AGAEDFSEEEDSYEVITAPDDFSAVREALEALKIPMMEADVTMIPQTWVELSNEDDIK
                     KLNRILDLLDEDDDVQAVYHNWDE"
     gene            complement(1273..2652)
                     /locus_tag="ACZPCL_09270"
     CDS             complement(1273..2652)
                     /locus_tag="ACZPCL_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1162691.1"
                     /translation="MGGILKSTWEMLHDGRFLKKALMIACPVALQGMLNTIVNMVDTL
                     MIGGLGSTAIAAVGLANKVFFVFSLLVFGIVSGGGILAAQFWGSGDVKNIRKVLGLCV
                     MLAMVGAMFFVVPALTAPRMVMRIFTTSEGSIELGAKYLRIAALSYPFIALTNVYVAM
                     LRATNKVVFPVICSCIAIVVNICLNYVLIFGKFGAPTMGVAGAACATLTARCIEVTLV
                     LFYVYGKKLPLACGPKGMFGWHKAFITQFLETSAPVIANEFMWGLGTTMYSLAYGRMS
                     DDAVAAITIATTIQDIAVVLFQGLSAATAVILGNELGAGKLKRAEKYATQFFILQFIV
                     TITAGVCLILGRWKIIGIYSITPEVAMDVSQCLLVFVAYMPAKMFNYVNIVGVLRSGG
                     DTRMCLFLDTSGVWCIGVPLAFLGALVWHLPIYGVYALVMSEEVYKLILGYIRYRQKK
                     WLKNLAIQV"
     gene            complement(2725..3072)
                     /locus_tag="ACZPCL_09275"
     CDS             complement(2725..3072)
                     /locus_tag="ACZPCL_09275"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR1162692.1"
                     /translation="MIIRRGDIYYADLRPVVGSEQGGVRPVLIIQNDVGNRHSPTVIC
                     AAITSRMNKAKLPTHVELNRGSCDMIRDSVILLEQVRTIDKQRLKEKICHIDGELLQK
                     VNEALMISLELHT"
     gene            complement(3228..4412)
                     /gene="alr"
                     /locus_tag="ACZPCL_09280"
     CDS             complement(3228..4412)
                     /gene="alr"
                     /locus_tag="ACZPCL_09280"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR1162693.1"
                     /translation="MRPYSRVYATVNLDAVASNMRSMRDNLPASTLIMGSVKADGYGH
                     GSVPVAKTIEPYVFGYAVATIDEGIILRRHGINKTILILGVTHESRYEDLLRYDIRTA
                     MFQYEKAKKLSDLALKQGKKAVVHLALDTGMSRIGMKADREHAKEAAAIAALEGIEVE
                     GLFTHFARADETDKSAYEEQYRRYKEFLGYLKELGVKIPIRHCSNSAGIVESLESNHM
                     DMVRAGIAIYGMYPSDEVDHNSVKLTPAMEIKSCITYIKEIEAGTAVSYGGTFVADHT
                     MKVATIPVGYGDGYVRSLSGKGDVLIHGKRAAILGRICMDQFMVDVTDIPDVQEDDEV
                     TLLGSDGAECITMEELAEKSGGFHYEMICDIAKRIPRVYLKDGKVVGTKDYFDDVFKG
                     FE"
     gene            complement(4587..6155)
                     /locus_tag="ACZPCL_09285"
     CDS             complement(4587..6155)
                     /locus_tag="ACZPCL_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HIR1162694.1"
                     /translation="MRTLLLGRKMKATDAKTIQEIGIPSMVLMERAAMAVAAEAEKAA
                     KKDEIVWAVCGSGNNGADGIAAARMLHLKGYPVCIYMAGDENRGTVELKTQLSIARKL
                     GISVKPWQEIETEKKGKHCCVIIDAVFGVGLGRPVEGNYKRCLDDLNQIREQEHTFCV
                     AVDIPSGIHADTGAVMGTALKADVTVTFGWEKMGTALYPGRSYAGKVLVKDIGFPEMA
                     LEGEEEEGGRPERAFAYEKKDLCHIPERKADSNKGTFGKVLIAAGSAGMCGAAYLSAL
                     TAYRAGAGLVKILTVEENRPILQGLLPEAIIASYTPDQLLQGREEFKEMIEEQMKWAS
                     VVVLGPGLGSEPYVEYLVEDILTSAYVPVILDADGLNTIASHPYLTSYYTENIIITPH
                     LGEMARLTGQTISQLKEDLVGAASAYASRYGVTCVLKDAATVVAGQEGGLYINTSGCS
                     AMAKAGSGDVLTGTIAGLLAIGMEEEEAARMGVYVHGLAGEKAAGAGSHSLLATELSQ
                     AVGDVMDEAVRRNG"
     gene            complement(6152..6529)
                     /gene="acpS"
                     /locus_tag="ACZPCL_09290"
     CDS             complement(6152..6529)
                     /gene="acpS"
                     /locus_tag="ACZPCL_09290"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009533692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR1162695.1"
                     /translation="MILGVGTDLVEIDRMRKACEKEHFVSRTFTEAESRQAGGSASKL
                     AGSFAVKEAVAKVFGTGFRTFMPGDIEVLRDELGKPYVRLYGGALEQFEKMGMEQIHV
                     SISNTNGLAMAFAVGEGRGEQKK"
     gene            complement(6546..7208)
                     /locus_tag="ACZPCL_09295"
     CDS             complement(6546..7208)
                     /locus_tag="ACZPCL_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR1162696.1"
                     /translation="MERKEISKAVISRLPRYYRYLGELIEEGVERISSNELSSRMKVT
                     ASQIRQDLNNFGGFGQQGYGYNVKYLYSEIAKILGIDRQHNVIIIGAGNLGQAIANYT
                     NFERRGFVIRGMFDINPKLIGLVIRGIEIRSVDDLETFIRENNIQIAALTIPKTKAPE
                     IADRLIKAGIKAIWNFAHTDLVVPDDVVVENVHLSESLMRLSYRVCNMQEKSDAEDTK
                     KE"
     gene            7434..>8516
                     /locus_tag="ACZPCL_09300"
     CDS             7434..>8516
                     /locus_tag="ACZPCL_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1162697.1"
                     /translation="MILSCSNICKSFGENDILKQVSFHIEDHEKAAIVGINGAGKSTL
                     LKVLIGKLNADDGVVTWAKGASIGYLAQHQDLEGAETIYDALLEVKKPVIQMEARIRS
                     LELEMKSASGDELETKLSEYSRLNHEFEMADGYSYQSEITGVLKGLGFTEDEFSKPIT
                     ALSGGQKTRVSLGKLLLTKPDILLLDEPTNHLDMESIAWLETYLRNYSGAVIIVAHDR
                     YFLDRVVTKIIELDMGHCTVFSGNYSAYSDKKAMLRDAAIRAYLNQQQEIRHQEAVIA
                     KLKSFNREKSIKRAESREKMLDKIERLEKPTQANDSMDIRLEPDVVSGNDVLTVTDLS
                     KSFDTQTLFTNVDFEIKRGERVAIIGN"
BASE COUNT         2156 a         2198 c         1623 g         2539 t
ORIGIN      
        1 actcagcgtt ctcggaaagg tctccctgct ctcttgcctc tttgatcttc tgagctactt
       61 cttttcttct gactaccttc aggttctgca gctcatcctc gtactgcttc agtccggcat
      121 atgtcaaaat gtgtttcttg tctgccattt ttcctgcttc cttccttttg ctatttgctt
      181 acaacatgca ttcaggggca tcttttaatc tgcccattgc aatctatgcg ttatctgcac
      241 ctttcatgtc cctgtttttg tttcacgaaa aatgtcttca gtatctctta aaatacccca
      301 cacattcagt gtattatata ggaaaaacca gtcattgtca agtgcagcca caggcgcaaa
      361 aaaccctttc ccaccgcaaa aagcctgcgg caggaaagag ttttatatgt gctaaaacgt
      421 ttttaatcac tcatcccagt tatgatatac agcctgaacg tcatcatcct catctaacag
      481 atccaggata cggtttaatt tcttaatatc atcttcattg gaaagctcta cccatgtctg
      541 tgggatcatg gtaacatctg cttccatcat cgggatcttt aatgcttcta atgcttcacg
      601 tactgcactg aaatcatctg gtgcagtgat tacttcgtag ctgtcttctt cctcggagaa
      661 gtcctcagcg cccgcatcta atgcagccat catcagttcg tcagcatcca tctcgcactc
      721 ttctttgtcg atgatgatct gacccttctt atcgaacata taggaaacac ttcctggagt
      781 tcctacgttg ccgccgccct ttgtaaatgc gcttctgaca tttgctgcag tacggttctt
      841 gttgtctgtc agagtatcta cgataatagc aacaccactt gggccgtagc cttcgtatgt
      901 aagtgtttcg tagtttacgg aagctgcatc gcctgctgcc ttcttgattc cacggtcaat
      961 ggtatcgttt ggcatgttgt ttgcttttgc ctttgcaata acatcacgaa gcttgctgtt
     1021 gtttgccgga tctggtccgc cctcttttac agctactgct aactctctgc cgatcacagt
     1081 aaagatctta cctttagcag catcgttacg ctcctttttg tgtttgatat tagcaaattt
     1141 tgaatgtcct gacattggta taacaccctt tctttatttc ttgtttcttt cgtttacgaa
     1201 attttacaat atcatctctg atattacatc ttttctatac taacacgatt ttttttatct
     1261 gacaagataa aatcagacct ggatcgccag atttttaagc cattttttct gacggtagcg
     1321 tatatagccc aggatcagct tataaacttc ttctgacata acaagggcat agaccccgta
     1381 aataggcaga tgccatacta atgcgcctaa aaacgccagc ggaactccaa tgcaccatac
     1441 accgctggta tccagaaaca ggcacatcct ggtatcacca ccgcttcgca gtacacctac
     1501 gatatttaca tagttaaaca tctttgcagg catataagca acaaatacca gcagacactg
     1561 gcttacatcc atggctactt ccggtgtaat gctgtaaata ccgatgatct tccaccttcc
     1621 aagtatcaga cacacccctg ctgttatagt aacaataaac tgcaggataa aaaactgggt
     1681 agcatatttt tctgcacgtt ttaacttacc agcaccaagt tcatttccca ggataactgc
     1741 tgtggctgcg cttaagccct ggaataatac caccgcaatg tcctgaatgg tggttgcaat
     1801 agtaatagct gccactgcgt catcgctcat acgtccatac gccagggaat acatggtagt
     1861 gcccagtccc cacataaatt catttgcaat gactggggcg gatgtttcca aaaactgagt
     1921 gataaacgcc ttatgccagc caaacattcc cttcggtccg caggcaagag gcagtttctt
     1981 cccatataca tagaaaagta ccagagtcac ctcaatgcat ctggcagtaa gtgtagcgca
     2041 ggcagcaccg gctacgccca tggtcggtgc gccaaacttt ccaaaaataa gcacatagtt
     2101 taagcagata ttaacaacaa tggcaataca gctgcagatc accggaaata ctactttatt
     2161 ggtagcgcga agcattgcca catatacatt ggtaagcgca ataaacggat agcttaaagc
     2221 tgcgatccga agatactttg cccccagttc aatgcttccc tcactggtgg taaagatcct
     2281 catgaccatt ctgggagccg taagggccgg gaccacaaaa aacatggctc ccaccatagc
     2341 aagcatcaca caaagtccta aaaccttcct gatatttttc acatcaccgc tgccccagaa
     2401 ctgggctgct aaaataccac cgccgctgac aattccaaaa accagcagtg aaaacacgaa
     2461 aaacacctta tttgcaagtc caacggctgc gatcgctgtt gatcccagtc ctccgatcat
     2521 aagggtatct accatattta caatggtatt taacattccc tgtagtgcca ccgggcatgc
     2581 gatcataagt gcctttttta agaagcgccc gtcatgaagc atttcccagg tactttttaa
     2641 tattcctccc atttttatct ccattatctg ttgttgtctt tacataagaa cagcctggaa
     2701 aagcaggact gtaccactca cagcctatgt atgcagttcc agacttatca ttaatgcctc
     2761 atttaccttc tgtaacagtt ctccgtcaat gtgacagatc ttctccttta acctctgctt
     2821 gtcaatggtc ctgacctgct ctaaaaggat cacggaatcc cgtatcatgt cacagcttcc
     2881 cctgtttaat tctacatggg taggcagctt cgccttgttc atccttgatg tgatcgctgc
     2941 gcagatcact gtcggactgt gccggtttcc cacatcattc tggatgataa ggaccggccg
     3001 cacaccgccc tgttcagacc ctacaacagg tcttagatct gcgtaataaa tatctccgcg
     3061 tctgatgatc acagccttca ctctccgtca tattcttacc cttagtattg acacattttt
     3121 aaaatgtata cactactgtt atcatatgag gagaaatgct ttccggagcc tgtttacccg
     3181 tatatcacga atttaatatt aaaaaactta ttgtaaccta ctgaaaatta ctcaaatccc
     3241 ttaaatacat catcaaaata atctttggta cccactactt ttccgtcttt taagtaaact
     3301 ctcggtatac gttttgcgat atcgcagatc atttcataat ggaaaccgcc ggacttttct
     3361 gccagttctt ccatcgtaat gcattcagcc ccgtcactgc ctaaaagggt aacttcatca
     3421 tcttcctgta catccgggat atctgttaca tctaccataa actgatccat gcagatacgg
     3481 cctaaaatag ctgctctctt gccgtggatc agcacatctc cttttccgga aagggaacgg
     3541 acataaccat ctccataacc tacaggaatg gttgctactt tcatggtatg gtctgccaca
     3601 aaggtgccgc catagctgac tgctgtaccg gcttcaatct ccttgatgta ggtaatgcag
     3661 cttttgatct ccatagctgg tgtcagtttt acgctgttat gatctacctc atcagaagga
     3721 tacatgccat aaatggcaat accggctctt accatatcca tatgattgga ctctaatgac
     3781 tcaacgatac cggcactgtt ggagcagtgg cggatcggga tctttacccc cagttcctta
     3841 aggtagccta aaaattcttt ataacggcgg tactgctctt cataagcgga tttatctgtc
     3901 tcatctgctc ttgcaaaatg ggtaaacaga ccttctactt caatgccctc taaggctgca
     3961 atggctgctg cctcttttgc atgttctctg tctgctttca taccgatccg gctcatacct
     4021 gtgtccagtg ccagatgcac cactgccttt ttgccctgtt ttaatgccag gtctgacagt
     4081 ttttttgctt tttcatactg gaacatggca gtgcggatat cgtagcgcag cagatcctca
     4141 tagcgtgact catgggtcac acccagaata aggatggtct tattgatccc atgtctgcga
     4201 aggatgatgc cctcatcaat agtagctact gcatatccaa acacataagg ttcaatggtt
     4261 tttgccacag gaacgcttcc atgtccatag ccatctgctt tcaccgatcc catgatcagg
     4321 gtagatgcag gcagattgtc ccgcatggat ctcatattag aagcaacagc atccaggttt
     4381 actgttgcat atactctgct gtaaggtctc atatatgttt tatttcctcc cggcgtattc
     4441 atgccttcct aaacgatctc tttctgaaaa cgcactaaag tgcgctaagc tttaattata
     4501 caccatacgc cgcatttttc aagaaggttt cccagtcatt caatcagttt ttgccaacat
     4561 atttattctg ctgcaactgg tacggtttac ccattgcgtc tcactgcctc gtccataaca
     4621 tcccctactg cctgggataa ttctgtggcc agcagactgt ggcttcctgc cccggctgct
     4681 ttctctcctg ccagaccgtg aacatacaca cccatgcggg ctgcttcttc ctcttccatg
     4741 ccaattgcta aaagtcccgc gatcgtaccg gtaagcacat ccccggaacc ggcttttgcc
     4801 atggcactgc agccgctggt attaatataa aggccgcctt cctggcctgc aactaccgta
     4861 gctgcatcct ttaatacgca ggtaactcca taccgggatg cataagcact ggctgctccc
     4921 accagatctt cttttagctg gctgatggtc tgtcctgtaa gtcttgccat ttcaccaaga
     4981 tgtggggtaa tgataatatt ttctgtataa taagaagtaa gataaggatg ggatgcaatg
     5041 gtatttaacc catctgcatc caggatcacc ggaacatagg cactggtaag tatatcctct
     5101 accagatatt ctacataagg ctcacttcca agtcccggtc ctaaaaccac tacacttgcc
     5161 catttcatct gttcctcgat catttcttta aattcttctc ttccctgaag aagctgatcc
     5221 ggagtatagg atgcaatgat cgcctccgga aggagtccct gaaggatggg acggttttct
     5281 tctactgtga ggatctttac cagtccggct cctgctctgt aggcagtaag ggcacttaaa
     5341 taggcagcac cacacatacc tgcacttccc gctgcgatca gcacttttcc aaaagttcct
     5401 ttgttggagt ctgcttttct ttcaggaatg tggcaaagat ctttcttttc ataggcaaaa
     5461 gcccgttccg gtctgccgcc ttcttcctct tccccttcta atgccatttc aggaaagccg
     5521 atatctttta ccagcacttt tcctgcataa cttcttcccg ggtaaagggc agttcccatt
     5581 ttttcccagc caaaagtcac agtcacatct gcttttaaag cagttcccat aacagcgcct
     5641 gtatctgcgt ggataccgga aggaatgtct actgccacac agaaagtatg ctcctgttct
     5701 cttatctggt tcaggtcatc taaacatctt ttataatttc cttctaccgg acggccaaga
     5761 cctactccga atactgcatc aatgataacg caacagtgtt ttcctttctt ttctgtctcg
     5821 atctcctgcc atggttttac agaaattccc agttttcggg caatggaaag ctgtgttttt
     5881 aattccactg ttcctctgtt ttcatcccct gccatataaa tgcatacggg atagcctttt
     5941 aaatggagca ttctggccgc tgcaataccg tctgctccgt tattgccact gccgcacaca
     6001 gcccagacga tctcatcttt ttttgctgcc ttttctgcct ctgcagccac tgccatagcc
     6061 gcccgttcca ttaataccat agacggaata ccgatctcct gaatggtttt ggcatccgtt
     6121 gctttcattt ttcttcctaa taaaagagtc ctcatttttt ctgctctcct cttccttctc
     6181 ctactgcaaa agccattgcc agtccatttg tattggaaat agaaacatgg atctgctcca
     6241 ttcccatttt ttcaaactgt tctaaagctc caccataaag acgtacatag ggttttccca
     6301 gttcatcccg caaaacctca atatctcccg gcataaaggt gcgaaaacct gtgccaaaca
     6361 ccttggctac cgcctctttt acagcaaaag agccggcaag cttagaggca gaccctcctg
     6421 cttgccggct ttccgcctct gtaaatgtgc gtgacacaaa atgttctttt tcgcaggcct
     6481 tcctcatacg gtcaatttcc accagatccg ttcctactcc cagtatcatc acgtttactc
     6541 tctttttatt ctttttttgt atcctctgca tcagactttt cctgcatgtt gcacacacgg
     6601 taagacagac gcatcagact ctctgataag tgtacatttt ctactactac atcatccggc
     6661 actaccagat ctgtgtgggc aaagttccag attgccttga tgccggcttt gatcaatcgg
     6721 tcagcaatct ccggagcctt tgtctttgga atggtaagtg ctgcgatctg gatattattt
     6781 tccctgataa aggtttccag atcatccaca gaacggatct caatgccgcg gatcaccagt
     6841 ccgatcagct ttggattgat atcaaacatt cctctgataa caaatcctct ccgctcaaaa
     6901 ttcgtataat tggcaatggc ctgtcccaga ttacctgcac caatgatgat cacattgtgc
     6961 tgacggtcaa ttccaaggat ctttgcgatc tctgaataaa gatattttac gttatatccg
     7021 tagccctgct ggccaaagcc accaaaattg ttaaggtcct gacggatctg agacgccgtt
     7081 accttcattc tgctgctcag ttcattagaa gaaatacgct ctactccttc ctcgatcagc
     7141 tcgccaaggt agcgataata tctgggcagt cttgaaatta ctgcttttga tatctctttg
     7201 cgttccattt taaccctctc cttcttgttc atgattcacc cccttgcatt atcggggtat
     7261 gataactcta gtatccatta taatcagacg ttaaataaat gtcaaatctg tttttcagta
     7321 tgttcttgaa aaaattattt tcctatatta tactgtttgg tgcgaaccgg ctcattttgt
     7381 cgggaaaata cttaatttta ctacattttt actacgtttt taaggagttt atcatgatat
     7441 tatcctgcag caatatctgc aagtcatttg gggaaaatga cattttaaag caggtttcct
     7501 tccatataga agaccacgaa aaggcggcca ttgtaggcat taatggtgcc ggcaagtcca
     7561 ctcttttaaa agtgcttatc ggaaaactga acgctgatga tggcgttgtt acctgggcga
     7621 aaggtgcttc catcggttat ctggcacagc accaggactt agagggagcc gagactattt
     7681 acgatgcact tttagaggtg aaaaagccgg tgatccagat ggaggcccgc attcgcagcc
     7741 tggaactgga aatgaaatcc gcttccggtg atgaattaga gaccaagctt tctgaataca
     7801 gccgtttaaa ccatgaattt gaaatggcag acggctattc ctatcagagc gagatcaccg
     7861 gcgttttaaa gggacttgga tttacagagg atgagttttc caaacccatc actgctcttt
     7921 ccggcggcca gaagacccgt gtttccttag gcaaactgct ccttaccaaa ccggacatcc
     7981 tgctccttga cgagccgacc aaccacctgg atatggagtc catcgcatgg ttagaaactt
     8041 atctgagaaa ctacagcggt gctgtgatca tcgttgccca tgaccggtat ttcctggacc
     8101 gtgtggtaac aaagatcatt gagctggaca tggggcattg tactgtattt tcaggcaatt
     8161 attctgccta cagcgataaa aaggccatgc tacgcgatgc tgccatccgt gcctatttaa
     8221 accagcagca ggagatccgc catcaggagg ctgttatcgc caagttaaaa tctttcaacc
     8281 gtgaaaaatc cattaaacgt gcagaaagcc gtgaaaaaat gctggataag atcgagcggt
     8341 tagaaaagcc tacccaggca aatgattcta tggatatccg cttagagccg gatgtggtca
     8401 gcggaaatga tgttctgacc gttacagatt taagcaaatc ctttgacaca cagaccttat
     8461 ttaccaatgt ggactttgag atcaagcggg gggaacgtgt tgctatcatt ggaaat
//
LOCUS       DBMDUA010000120         8364 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3832_length_8364_cov_2.481060, whole genome shotgun sequence.
ACCESSION   DBMDUA010000120 DBMDUA010000000
VERSION     DBMDUA010000120.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8364)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3832_length_8364_cov_2.481060"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="ACZPCL_09305"
     CDS             complement(<1..786)
                     /locus_tag="ACZPCL_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR1162698.1"
                     /translation="MELETGKKLRTGAVIVAAGHKSKNSVFKPLLPVGDSTAIRRIII
                     TLKRAGVSPIVVVTGDKADELEKHISNLQVICLRNEQYAVTQMFSSICMGLNYIEDLC
                     DRVFVLPAKFPVFLRETVQRLMQSDADVARPMFDGHRGHPVLISSSVLPSIVSYQGSD
                     GLRGALRQAAETFQEENIPVEDKGIIQAIETEGDSSVDFIRKQQIQVHPRIQLGLERD
                     DIFFNAQTAHFLQLTSHTGSMQTACKQMHMSYTKGWKILREAEK"
     gene            complement(815..1927)
                     /locus_tag="ACZPCL_09310"
     CDS             complement(815..1927)
                     /locus_tag="ACZPCL_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HIR1162699.1"
                     /translation="MAIQTEHSLQLGVEIKKKLKEFDLDISFSAGKGCLGILGPSGCG
                     KSMTLKSIAGIVTPDSGRIALQYAKGEAAGGRVLYDSALKINEKPQMRRVGYLFQNYA
                     LFPNMSVEENIAAGLKGMEKKKAKVGEMIERFRLTGLEKRYPGQLSGGQQQRVALARI
                     LAYEPEALLLDEPFSAMDTYLREGLRLELSKVLADYDGVSVMVTHDRDEAFQLCKNIM
                     LLDKGHVLIAGNSRKIFQDPVTCKAARLTGCKNISKIERIGEYRVRALDWDNLEFVTD
                     RTVGDDITAIGIRAHDFEPLAGEKAKTKKEAGEENLIPVVKPAISEMPFEWYITLSNG
                     LWWKKEKTIHTHDAAGVVPEYLRADPSAILLLKGEL"
     gene            complement(1950..2618)
                     /gene="modB"
                     /locus_tag="ACZPCL_09315"
     CDS             complement(1950..2618)
                     /gene="modB"
                     /locus_tag="ACZPCL_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR1162700.1"
                     /translation="MDWSPILISMKTASLSIFITFFIGLFAAWAVVSLKNETLKVICD
                     GILTLPLVLPPTVAGFFLLYLFGVKRPIGQFFIEYFSVKIAFSWIATVLAAVTMSFPL
                     MYRSARGAFEQVDQNLVAAARTLGMSEWAVFWKVLFANATPGILSGGVLAFARGLGEF
                     GATAMIAGNIAGKTRTLPMAVYSEVAAGNMDAAYSYVAIIVVIAFISVVLMNWGALIR
                     SRKF"
     gene            complement(2714..3589)
                     /gene="modA"
                     /locus_tag="ACZPCL_09320"
     CDS             complement(2714..3589)
                     /gene="modA"
                     /locus_tag="ACZPCL_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1162701.1"
                     /translation="MKKQICLVMAAMMAAGMLAGCSGSAKETTAAATEAATTAEETKA
                     EETTAEETTAAASGEETEILVAAAASLKNAYEDKLIPMFEEANPGVTVKGTYDSSGKL
                     QTQIEEGLEADVFMSAAKKQMTALDEEGMIASDTITDLLENKIVLIVPTGNEKKLEKF
                     EDIENADSIALGDPASVPAGQYAQEALTNLGIWDKIQDKVSFGTNVTEVLNQVAAASA
                     DAGIVYATDAASMADQVEVVAEAPEGSLAKKVIYPVAVVKGTAHEEVAKEFVAFLQTD
                     KAITVFEEYGFSSAQ"
     gene            complement(3763..4728)
                     /locus_tag="ACZPCL_09325"
     CDS             complement(3763..4728)
                     /locus_tag="ACZPCL_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HIR1162702.1"
                     /translation="MEITGKRGPEGVVKAVCTSEKKGVEKHSVPEGHFLKDFGIEGDA
                     HAGKWHRQVSLLSYDKVKEFNERGAQVEDGAFGENLVVEGIDFRSLPVGMMIYIGDVV
                     LRMTQIGKECHSHCAIYKRMGECIMPREGVFAEVLQEGTIHPGDTVITCYPDENRPFQ
                     AAVITLSDKGAKGERVDESGPAAKEMLEQAGYEVVETLILPDEPAMLKTQLMRLADGR
                     QLDLVLTSGGTGFSMRDQTPEATMAVADRNAPGIAEAIRYKSMAVTDRAMLSRGVSVI
                     RKKTLIVNLPGSPKAVKESLGFILDSLDHGLKILRGSASECARRD"
     gene            complement(4735..5802)
                     /gene="moaA"
                     /locus_tag="ACZPCL_09330"
     CDS             complement(4735..5802)
                     /gene="moaA"
                     /locus_tag="ACZPCL_09330"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR1162703.1"
                     /translation="MKDRMGREIDYMRFSITDRCNLRCKYCMPDGIDCMPRWDVLSLE
                     EFEAIAIAAASLGIKKIKVTGGEPLARKSCPQLVKMLKNIPGIEKVTLTTNGVQLDQY
                     LDQLLDAGLDAINVSLDTVDPVLYEKITGRDKLADVMRFIEKAESLPIPVKINAVSID
                     FRQLQENGKATELSPQENWQQMAELAKHYPVDVRFIEMMPIGYGKQFKTIDHNWLLEN
                     MKKVYPDLEEDTRVHGFGPAVYYKVPGFKGSIGLISAIHGKFCSTCNRIRLTSQGHVK
                     SCLCYDEGTDLRSILREGQERPDKDTHYIWPEGRDVRDEKLQEKLREALAKAIYEKPA
                     AHCFEKPGEITEAHNMISIGG"
     gene            complement(5802..6290)
                     /gene="moaC"
                     /locus_tag="ACZPCL_09335"
     CDS             complement(5802..6290)
                     /gene="moaC"
                     /locus_tag="ACZPCL_09335"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR1162704.1"
                     /translation="MEFTHFDENGNALMVDVSEKAETKREATARGSIYMSPECLKKVK
                     EGTMAKGDVLGVARVAGIMGAKKTSELIPLCHLLNLTKLTVDFTIKEETNEIEAACTA
                     RTTGKTGVEMEALTGVNIALLTIYDMCKAVDKSMEMGNIYLEHKSGGKSGEFVNPRKQ
                     GI"
     gene            complement(6296..7354)
                     /locus_tag="ACZPCL_09340"
     CDS             complement(6296..7354)
                     /locus_tag="ACZPCL_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HIR1162705.1"
                     /translation="MKLIKTVDAEGAVLCHDITQIIKGVTKDAVFRKGHIVTKEDIPV
                     LLSVGKDQLYVWEKEEGMLHENDAAEVLCAMCKGEHMERSQAKEGKIELSAACDGLLK
                     INNNGLKAVNGFGQMMIATRHGNFAVKKGDKLAGTRIIPLVIEEEKMTKAKEAAMAAT
                     GNKPILNLLPFKHKKVGIVTTGNEVFYGRIKDTFTPVIEGKMAEFDTEIIDHVTWNDD
                     DTKVTASILDMIKKGADIVVCTGGMSVDPDDKTPLAIKNTGADIVSYGAPVLPGAMFM
                     LAYYQVTEGENPRTVAIMGLPGCVMYAKRTIFDLVLPRVMADDQVTAEELAALGQGGL
                     CLNCPVCTFPNCGFGKGV"
     gene            complement(7534..7842)
                     /locus_tag="ACZPCL_09345"
     CDS             complement(7534..7842)
                     /locus_tag="ACZPCL_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HIR1162706.1"
                     /translation="MNKKIKTEAVDHLFDAILTLKDPEECYKFFEDVCTINELLSLSQ
                     RYEVAKMLREGKTYLEIAEKTGASTATISRVNRSLNYGSDGYDMVFERLLGEEKKEEQ
                     "
     gene            8169..>8364
                     /locus_tag="ACZPCL_09350"
     CDS             8169..>8364
                     /locus_tag="ACZPCL_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1162707.1"
                     /translation="MTNYKPKDFAELLGVSVKTLQRWDREGTLKANRTPTDRRYYTYG
                     QYLQFKGINTEDDTRQVVIYA"
BASE COUNT         2107 a         2099 c         1564 g         2594 t
ORIGIN      
        1 tttttctgct tctcttaaga tcttccagcc ttttgtgtat gacatatgca tctgcttaca
       61 ggctgtctgc atggaacctg tatgggaagt aagctgtaag aaatgggctg tctgggcatt
      121 aaaaaatata tcatcccgtt ctaagccaag ctggatcctt ggatggacct ggatctgctg
      181 tttccggata aaatcaacgc tgctgtctcc ctctgtttct atagcctgaa tgatcccctt
      241 gtcctcaacg ggaatatttt cttcttgaaa ggtttctgct gcctgacgaa gggctcctct
      301 taaaccatca cttccctgat aagatacaat agagggaagt acagaagaag aaataagaac
      361 cggatgacct ctgtggccgt caaacatggg acgggccaca tctgcatctg actgcatcag
      421 acgttgcaca gtttcccgta agaaaaccgg aaacttcgcc gggagcacaa acacgcggtc
      481 gcaaagatct tctatatagt taagccccat acagatactg gaaaacatct gggtcacagc
      541 atactgctca ttcctaaggc agatcacctg gagattggaa atatgttttt ccagctcatc
      601 tgccttatcc cctgttacta ctacgatagg ggaaactccc gcccgcttta aggtaatgat
      661 aatgcggcgg atcgccgtac tgtcacccac cggaagcaag ggtttaaaaa cagaattttt
      721 acttttatgt cctgctgcca caataactgc tcctgtgcga agcttttttc cagtttctaa
      781 ctccatgttt cccatttcct ttcttatgtc tgttctacag ttctcccttt aaaagcagga
      841 tcgctgacgg atctgcccgg agatattccg gtactacacc tgctgcgtca tgggtgtgga
      901 tggtcttttc ctttttccac cataagccgt tactaagagt aatataccac tcaaatggca
      961 tttcggaaat agctggtttt acaacaggga tcaggttttc ttctcccgcc tcttttttgg
     1021 tctttgcttt ttctcctgcc aaaggctcaa aatcatgggc acggatacca attgctgtaa
     1081 tatcatctcc aacggtccgg tctgtaacaa attccagatt gtcccagtca agggcgcgga
     1141 cccggtattc gccaatacgc tcaatttttg aaatattttt acatccggtc agtcttgctg
     1201 ccttacaggt aacaggatcc tggaagatct ttctggaatt tccggcgatc agcacatgtc
     1261 ccttgtctaa cagcataata tttttgcaaa gctggaaggc ctcgtctctg tcatgggtta
     1321 ccataacaga aacaccgtca tagtcagcta atactttaga aagttccaga cgcagaccct
     1381 ctcttaagta tgtatccata gcagaaaaag gctcatctaa aagcagtgct tccggctcat
     1441 aagctaaaat acgggctaac gctactctct gctgctgacc gccggaaagc tgtcccggat
     1501 aacgtttttc aagaccggtc aggcggaaac gttcgatcat ttctcctact tttgcctttt
     1561 tcttttccat tcctttaaga ccggctgcaa tattttcctc aacagacatg tttgggaaaa
     1621 gggcataatt ctggaacaga tagcctacac ggcgcatttg tggtttttca ttgatcttta
     1681 aagccgagtc atagagaact cttcccccgg cagcttcccc ttttgcatat tgtaaggcaa
     1741 tacggccgct gtccggggta acgatacctg caatggattt aagagtcata ctctttccac
     1801 agccggaagg acctaaaata cccagacacc cttttcctgc actgaatgaa atgtccaggt
     1861 caaattcttt taattttttc tttatttcaa ctccaagctg gagagaatgt tctgtttgaa
     1921 tagccattct ttttacctcc gagagaacat taaaacttcc gtgagcggat caatgcgccc
     1981 cagttcataa gaacaacgga aataaatgca atgacaacaa tgatagctac ataagaatat
     2041 gctgcgtcca tatttcctgc agctacttct gaatagaccg ccataggaag tgttcttgtc
     2101 tttcccgcaa tgtttcctgc gatcatggca gttgcgccaa attcacctaa acctctggca
     2161 aaagcaagaa cgccgccgct taagataccc ggtgttgcat ttgcaaacag caccttccag
     2221 aatacagccc attcgctcat tcccagtgtt ctggccgctg caaccaggtt ctgatctacc
     2281 tgttcaaaag cacctctggc agaacggtac attaacggaa atgacatggt aacagctgcc
     2341 aagacagtcg cgatccagga aaaggcgatc tttacactga aatattcaat aaaaaactgt
     2401 ccgataggac gttttacacc gaaaagatat aacaaaaaga agcctgctac tgtaggcggc
     2461 aggactaaag gcagtgtcag gataccatca cagataacct ttaatgtttc atttttcagg
     2521 cttaccactg cccaggctgc aaacagcccg ataaaaaatg tgatgaaaat agacagactg
     2581 gctgtcttca tggaaatcaa aattggggac cagtccattt agaacctctt ttcttatgag
     2641 atttatatgg gaaattgatt aaaaccggga tactgtaact tactttacaa catcccggtt
     2701 ttatagttac tgactactgc gcactggaaa atccatactc ttcaaatact gttattgcct
     2761 tatcagtctg taagaaagca acaaattcct ttgcaacttc ctcatgagca gtacccttaa
     2821 caacagctac aggatagata accttctttg caaggcttcc ttctggagct tctgcaacaa
     2881 cttctacctg atcagccatg cttgctgcat cagtagcgta tacgatacca gcgtcagcac
     2941 ttgctgctgc tacctggttt aatacctcag ttacgttggt tccgaaactt accttatcct
     3001 ggatcttatc ccagataccc aggttagtta aagcctcctg tgcatactgt ccagctggaa
     3061 cacttgcagg atcgcctaat gcaatgctgt ctgcattttc gatgtcctca aacttctcca
     3121 gcttcttctc atttccagtt ggaacgatca gaacgatctt gttctctaac agatcagtaa
     3181 tggtatcgga agcgatcatg ccttcctcat ccagagctgt catctgcttc ttagctgcgg
     3241 acatgaatac gtctgcttcc agaccttcct cgatctgtgt ctgcagtttg cctgagctgt
     3301 cataggttcc ctttacagtt acgcctgggt ttgcttcttc gaacattggg atcagcttat
     3361 cttcgtaagc gttctttaag cttgctgctg ctgcaactaa gatctcagtc tcttcgccgg
     3421 aagctgctgc tgtagtttct tctgcagttg tctcctctgc ttttgtttct tctgcggttg
     3481 ttgctgcttc agtagctgct gctgtggttt cctttgcgct gccggagcat ccagctaaca
     3541 ttcctgctgc catcatagct gccattacaa ggcaaatctg tttcttcata atctcatttc
     3601 tcctttaaat aaaaattgat ttatttatag cagtatcgtt atactcgttt gactatagcg
     3661 atcataagga gaacaccgcc tgcacggtat tcttcctccc gctgcaggcg gtacaaagat
     3721 actgtctata tgcgattttt aaaactttcc gttttccttt tcttaatctc ttctggcaca
     3781 ctcagaagca ctgccgcgaa ggatctttaa gccgtgatcc aaactgtcta aaataaatcc
     3841 caagctttct tttactgcct ttgggcttcc cggcaggttt acgatcagtg ttttctttcg
     3901 gatcacactg acacctctgc ttaacatggc tctgtctgtc actgccatgg atttataacg
     3961 aatggcttct gcaattccgg gagcattccg gtctgccact gccatggttg cttccggagt
     4021 ctggtctctc atggaaaaac cggttccgcc gcttgtaagc accagatcta actgtctgcc
     4081 gtctgccaga cgcattaact gtgtctttaa catagccggt tcatccggaa ggatcagcgt
     4141 ctctactact tcgtatcccg cctgctccag catttccttt gctgcaggac cgctttcgtc
     4201 tactctttct cctttggcac ctttatcact taaagtgata acagctgcct gaaaaggccg
     4261 gttctcatct ggataacagg taataacagt atctcccgga tgaatggttc cttcctgtaa
     4321 gacctctgca aacacgccct ctcttggcat gatgcattcg cccattcttt tataaatcgc
     4381 gcaatggctg tgacattctt tgccgatctg ggtcatgcgt aaaaccacat ccccgatata
     4441 gatcatcatt cctactggca gagaacggaa atcaatccct tctaccacca gattttctcc
     4501 aaaagcacca tcttctacct gagcgccacg ctcattaaat tcttttacct tgtcataaga
     4561 caaaagactt acctgacgat gccatttacc agcgtgtgca tctccctcga taccaaaatc
     4621 tttcaggaaa tgtccttccg gaacagaatg tttttcaaca cctttttttt cactggtaca
     4681 gaccgctttt acaacacctt ccggtcctct ttttccagtt atttccattt tatcttatcc
     4741 tccaattgaa atcatgttat gtgcctcggt gatctcacct ggcttttcaa agcaatgtgc
     4801 cgctggcttt tcgtagatcg cctttgcaag tgcttcccgc agcttttcct gcagcttttc
     4861 atcccttaca tctctgcctt ctggccagat ataatgggta tccttatccg gtctttcctg
     4921 cccttctctt aaaatacttc taagatctgt tccctcatca tagcagagac aggattttac
     4981 atgtccctgg gatgtcagac ggatgcggtt acaggtagaa cagaattttc cgtgaatagc
     5041 actgatcaga ccaatgcttc ctttaaatcc gggaacttta taataaaccg caggtccaaa
     5101 tccatgaact cttgtgtctt cttccagatc cggatagacc tttttcatat tttcaagcag
     5161 ccagttgtga tcaatggtct taaactgctt tccatagccg atgggcatca tttctataaa
     5221 acgcacatcc actggataat gctttgcaag ttctgccatc tgctgccagt tttcctgagg
     5281 ggaaagttct gtagcttttc cattctcctg taactgcctg aaatcaatgg aaactgcatt
     5341 gatcttaaca gggattggta agctttctgc tttttcaatg aacctcataa catcggctaa
     5401 cttatcccgg ccggttatct tctcataaag taccgggtct acggtatcca ggcttacatt
     5461 aatagcatcc agtcctgcat ctaatagctg atccagatac tgatctaact ggactccgtt
     5521 ggtagtcagg gtgacttttt caatgcctgg gatattttta agcatcttta ctaactgagg
     5581 gcagcttttc cttgccagtg gctctccacc ggtaactttg atctttttga tccctaatga
     5641 tgctgccgca atggcaatgg cttcaaattc ttccagagat aacacatccc atcttggcat
     5701 acagtcaatg ccatccggca tgcagtattt acagcgcagg ttacaccggt ctgtaatgga
     5761 aaaccgcatg tagtcgatct ctcttcccat tctgtctttc atcagatgcc ctgctttctg
     5821 ggattaacaa actcgccgct ttttccgccg gatttatgtt ccaggtaaat atttcccatt
     5881 tccatggact tatccactgc cttacacata tcgtaaatgg taagaagggc aatatttaca
     5941 ccggtcagtg cttccatttc cacacctgtc tttccggtag tacgggcggt acaggctgct
     6001 tcgatctcgt ttgtttcttc ttttatggta aaatccaccg tcagcttggt aagatttaac
     6061 aaatggcaca gcgggatcag ttcagatgtc ttcttagcac ccatgatacc ggctactctg
     6121 gcaactccca gtacatctcc ctttgccata gtcccttctt ttactttctt taagcactca
     6181 gggctcatat aaatgcttcc tctggcagta gcttcccgct ttgtctctgc tttttcactt
     6241 acgtccacca taagggcatt gccattttca tcaaaatgtg taaattccat ttctattaca
     6301 ctccctttcc aaaaccacag ttagggaagg tacagactgg acagttcaga cataagccgc
     6361 cctggcccaa agcagccagt tcttctgctg ttacctgatc gtctgccata actcttggaa
     6421 ggaccagatc aaaaatagtc ctctttgcat acataacgca gccaggaagt cccataatag
     6481 ctacagttct tggattttct ccctctgtta cctgataata agctaacatg aacatagctc
     6541 ccggaagtac cggtgcgccg taagatacaa tatcagcccc ggtatttttg atggcaagag
     6601 gggtcttatc atccgggtcc acactcatgc cgccggtaca gactacgata tccgcgcctt
     6661 ttttgatcat atcaaggata gatgctgtta cctttgtatc atcatcgttc caggtcacat
     6721 ggtcgatgat ctctgtatca aattctgcca ttttcccttc aataacaggt gtaaaggtat
     6781 ctttgatcct tccatagaaa acttcgtttc cagtggtaac gatacctact tttttatgtt
     6841 taaatggaag caggtttaag atgggcttgt ttcctgttgc cgccatagct gcttcttttg
     6901 cctttgtcat cttttcttct tcaatgacaa gtgggataat acgggttccc gctaatttat
     6961 cacctttttt aacggcaaaa tttccgtggc gggtagcgat catcatctga ccgaagccat
     7021 tgactgcctt taagccattg ttattgatct ttaataaacc atcgcaggct gcagacagct
     7081 cgatctttcc ttcttttgcc tgggaacgct ccatgtgttc ccctttgcac atggcacaaa
     7141 ggacttctgc agcgtcattt tcatgaagca tgccctcttc cttttcccat acataaagct
     7201 ggtcctttcc cacgcttaaa agtaccggga tatcttcttt tgtaacaatg tggccttttc
     7261 taaaaactgc atccttggtc acacctttga tgatctgggt aatatcatgg cagaggaccg
     7321 ctccctctgc gtctactgtt ttaattaatt tcatattttt atgtaacctc ccgttattat
     7381 tttctataaa taacgtacta ttagaataac actcacataa ctttttgtaa accttttacc
     7441 aaaaactttt tgcctatctt tttgcccatt taaaaagagc cctgcagcat acatagcctg
     7501 caaggctctt ttcatcccca atacattttc tttttactgc tcttcttttt tctcttctcc
     7561 aagaagccgc tcaaatacca tatcatatcc gtcacttcca taattcagtg aacggttcac
     7621 acggctgata gtagctgtag atgctcctgt cttttccgcg atctcaagat aggtctttcc
     7681 ttccctgagc atttttgcca cttcgtatcg ctgtgacagt gacagcagct cattgatggt
     7741 gcatacgtcc tcaaagaact tgtagcactc ttccggatct ttcagcgtga ggatcgcgtc
     7801 aaataaatga tctacggctt ctgttttgat cttcttattc atgatttttg ctccttatat
     7861 acgttaccgc ccccggacgg tcgttttaaa aataatacaa ttttttacgt ctgtataatt
     7921 ttaacatatt aaagtctgaa agtccatatc taaagtctat atcttttcat gaccttttct
     7981 taaaatccat atattccccc ggaaaaatgt gtggtatact aggaacgaac atcctgatgt
     8041 gctttctgaa cccttgtgaa agcacactca aactgtaagg agaaaacttt aagtaactga
     8101 ataagttgtc aaatgtgata atatgtgttt aaatatactc atagaggtat actatgaata
     8161 catcaaatat cacaaattac aagccaaaag attttgctga attgctaggt gtttctgtca
     8221 aaacgttaca gcgttgggat agagaaggaa ctctaaaagc aaatcgtact ccaactgata
     8281 ggcgttatta cacttacggc caatatcttc aattcaaagg cataaataca gaagatgata
     8341 cgcgtcaagt tgtcatttat gcca
//
LOCUS       DBMDUA010000121         8303 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3867_length_8303_cov_2.771275, whole genome shotgun sequence.
ACCESSION   DBMDUA010000121 DBMDUA010000000
VERSION     DBMDUA010000121.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3867_length_8303_cov_2.771275"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(73..1434)
                     /locus_tag="ACZPCL_09355"
     CDS             complement(73..1434)
                     /locus_tag="ACZPCL_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1162708.1"
                     /translation="MTNLIVEVSKYLMILLMAIYTYANFRFFSFPDLERKRKVCARQN
                     RAMFLLHFLAYVVMYLKTEDETLRRMLLVFYVGQVVFFLFYIYIYRFIYRNVSRLLVN
                     NCCMLLACGFIMLTRLSLDKGLDKAFKQFVIVAAAAILSWLVPFIMERFWQLYKFQWV
                     YAGIGLAALLVVWVAGNESFGAQLSLTVGGISIQPSEFVKISFVFFVASMFYQSLDFK
                     NICITTVVAALHVVILVLSKDLGSALIFFISYVIMLFIATGNWLYLIAAGVLGKGATS
                     IAYTLFDHVRRRFTAWKNPWADIDNTGYQITQSLFAIGTGGWFGMGLCQGMPNRIPVV
                     EKDFIFAAISEEMGAIFAICVLLICLGCFIQFMMIATQMQAMFYKIIAVGLGMEYVVQ
                     VFLTVGGVTKFIPSTGVTLPFVSYGGSSIVTTFLLFSIIQGLYIIKRNDEEEMEEMEA
                     QEE"
     gene            complement(1438..3666)
                     /locus_tag="ACZPCL_09360"
     CDS             complement(1438..3666)
                     /locus_tag="ACZPCL_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HIR1162709.1"
                     /translation="MNKRKAELLAPAGSFDSLKAAVAAGADAIYMGGSRFGARAYAQN
                     AAGEEMVEAIRYAHLHGCRLYMTVNTLFKEKELDELCDYMKPYYEAGLDGVIVQDLGA
                     LQVMKRAFPVMELHASTQMTVTSVYSAKMLKEMGCCRVVPARELSLEEISRIYKETGM
                     DIETFVHGALCYCYSGQCLMSSLIGGRSGNRGRCAQPCRLPYRVWEGDKAEGKSLNKE
                     NQKFVLSLKDLCTLDILPEILEAGVYSLKIEGRMKSPRYTAGVVRIYRKYLDLYETYG
                     KDGYYVDPEDKKELLDLFDRGGFTSGYYEKHNGKDMVALKEKPEFREGNQKLFDFLDA
                     NYVEKEKKEPVVGKAVFEEGKPAVLELTQGNVTVKVEGQICQSAQNQPATEEKVRKQL
                     NKTGATSFYFEQLDIELKGNIFLPVQALNELRREGFEKLTEKILAQWERKAESLVQTE
                     QELPADVQSASKNIHSFAFLTASLEEECQLEPALSCDAVKRIYIDADGFKPDQWGKIV
                     KQCEAKGKECFLTLPHIFRTHAMKFFSKNRKNLENAGFTGVLARTLEEVSWLKEEKIE
                     LPFALDASVYAWNREAVHTLKEMGPEFITMPWELNSRELKAVAKACERDGIPGELIIY
                     GHAPMMVSAQCVVKTLKGCTAKREHLIMKDRTGAGLPLKAHCNFCYNTILNPLPLSLF
                     GNEEAVRELNMASLRLSFTREGKEETQKILKAFAASFIEGREIVEPVKDFTRGHFRRG
                     VE"
     gene            complement(3747..4433)
                     /locus_tag="ACZPCL_09365"
     CDS             complement(3747..4433)
                     /locus_tag="ACZPCL_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR1162710.1"
                     /translation="MDTKNYTQVLIDGKIYTLGGSEDEAYLQKAASYVNEKITTMHAL
                     PGFSKQSADYQQLMLLLNIADDYFKTKETADQMEEQRDRMEKETYSLKHELVSTQMKL
                     DGVLKDLEERQRQLQELSAKLSKTERELRAAKKQAALAATSENGEEEVVEDEVVDTVI
                     EGDEPVSESVPALDETAAAQATESGFVQADMPMQNPSFQTKTGLSDQELARKALQAAR
                     KAGSHRNGRR"
     gene            complement(4489..5484)
                     /gene="ruvB"
                     /locus_tag="ACZPCL_09370"
     CDS             complement(4489..5484)
                     /gene="ruvB"
                     /locus_tag="ACZPCL_09370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR1162711.1"
                     /translation="MERRIITTEATEEDVRIETTLRPQCLSDYVGQEKIKSTLKIYIE
                     AAKSRGEALDHVLFYGPPGLGKTTLSGIIANEMGSRLKVTSGPAIEKPGEMAAILNNL
                     QEGDVLFVDEIHRLNRQVEEVLYPAMEDFAIDIMLGKESSARSIRLELPHFTLVGATT
                     RAGLLSAPLRDRFGVVQRMEFYTPEELRTIIQHSATILGVEIEPEGAIELAKRSRGTP
                     RLANRLLKRVRDFAQVKYDGVITKEVADFALDIMDVDKMGLDQNDRNILLMIIEKFSG
                     GPVGLDTLAAALGEDAGTLEDVYEPYLLMNGLINRTPRGRMATENAYRHLGLEMK"
     gene            complement(5501..6136)
                     /gene="ruvA"
                     /locus_tag="ACZPCL_09375"
     CDS             complement(5501..6136)
                     /gene="ruvA"
                     /locus_tag="ACZPCL_09375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR1162712.1"
                     /translation="MISYIKGALGAVEDDVIVVETGGIGLAVHVPLSLLEELPALGEE
                     VTVYTYFQVREDAMTLYGFLHRQDREMFKQLIGVNGIGPKGALGILSIMRPDDLRIAI
                     VSGDAKAISKAPGIGAKTAQRLILDLKDKVDLEEVMMASFGGGKEDTSGAGTGASVAG
                     MAAAAKEAVAALTALGYTNMEASKAVKKVEITENMSVEDILKASLKYLSFL"
     gene            6351..7454
                     /locus_tag="ACZPCL_09380"
     CDS             6351..7454
                     /locus_tag="ACZPCL_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HIR1162713.1"
                     /translation="MITIKHPIEFPNTYPLDRIGPLKDLLFFDIETTGFSGETSQLYL
                     IGCTYFDGFGWKLIQWFADTRQAEKELLDAFFEFLKRFKILIHFNGDRFDIPYLLKCC
                     ERLGLDYHFDDLISIDIFKKIKPYRKLLKLENMKQKSIEQFLGVHREDKYNGGQLIDV
                     YREYLHTHEKFLFDLLILHNADDLRGMPSILPILNYADLFESDFTLCSHKLYSYTDEA
                     GYSHPFVNLLWESPYSVPIPLEYTLNPLSLELHDNCLACNLTLYEGTLKYFYPDYQNY
                     YYLPLEDTAIHKSIGEYVDRSARKKATAKNCYSKKEGLFLPQFGSLWEPSFKEEYKSS
                     LTYTLYDDKTLWNDTVKNSHYAHEIFNYLTGQL"
     gene            complement(7429..8295)
                     /locus_tag="ACZPCL_09385"
     CDS             complement(7429..8295)
                     /locus_tag="ACZPCL_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HIR1162714.1"
                     /translation="MISEINSFTAYLQEVKHTAKNTEVSYHRDLLQMAGFMEEMGITQ
                     VEKVTKTSLNSYILYLEKEGKAAATISRELASIKAFFSYLVKERKINRDPAEFLRAPK
                     IERKIPVILTIEEIHALLAQPGNTTAKELRDKAMLELLYATGIRVSELISLKLEDVNM
                     QIGYITCHDGSKDRMIPFGKGAKQALRTYLDKARSSLLKGRACEWLFTNCSGQPMSRQ
                     GFWKLIKYYGEKTGIKEDITPHTLRHSFAAHLIGDGADIHAVQAMLGHGDTGASQMYA
                     VYAAATAARSDS"
BASE COUNT         2122 a         2146 c         1556 g         2479 t
ORIGIN      
        1 gcatctgtag ggaccggttt ggggatctgt ttatcggcgg ccgttctctt cgcgtacttt
       61 ttttcagttc tttcattctt cctgcgcctc catttcttcc atctcttctt catcattccg
      121 cttgatgata taaagcccct ggataatact gaataacagg aatgttgtca caatggaact
      181 tcctccgtaa cttacaaacg gaagtgtcac acctgtagat gggataaatt tagtcacacc
      241 tcccactgtg agaaagacct gtaccacata ttccattcca agacccactg caatgatctt
      301 gtaaaacatg gcctgcatct gggttgcgat catcataaac tggataaaac agcccagaca
      361 gatcagaagg acgcagatag caaagattgc tcccatttct tcggagatag cagcaaaaat
      421 aaaatccttt tccactaccg ggatcctgtt tggcattccc tggcacagtc ccatgccaaa
      481 ccagccacct gttccaatag caaacaggga ctgggtgatc tgatatcctg tgttatcaat
      541 atctgcccat ggatttttcc atgctgtaaa tctccgtctt acatggtcaa aaagtgtata
      601 tgcaatggat gtagcacctt ttccaagaac acctgccgcg atcagataca gccagtttcc
      661 tgtagcaata aacagcatga tcacatagct gataaaaaag atcagggcgc ttcccaggtc
      721 ttttgacaac accaggatca ctacatgaag agctgccacc actgtggtaa tgcatatgtt
      781 cttaaaatcc agtgactgat aaaacatgga cgctacaaaa aatacaaaac tgatctttac
      841 aaactctgat ggctggatgg agattccgcc aacagtcagt gataactgag cgccgaaact
      901 ttcatttcct gctacccata caactaaaag cgcagccagc ccgatccctg catacaccca
      961 ctggaattta taaagctgcc agaaccgttc catgataaaa ggcaccagcc aggaaaggat
     1021 cgcagccgct gctacgatca caaactgctt aaaagcctta tccagtccct tatctaaaga
     1081 cagtctggta agcatgataa aaccacaggc caggagcata caacagttat ttaccagaag
     1141 tcttgacaca ttcctgtaaa taaaccggta gatgtaaatg taaaacagga aaaacaccac
     1201 ctgtcccaca taaaatacca gcagcatccg ccttaacgtt tcatcttctg ttttcagata
     1261 catcaccaca tatgccagaa aatgcagcaa aaacatggcc cggttctgcc ttgcacacac
     1321 ttttctcttc cgttccagat ccgggaaaga aaagaaacgg aaattggcat aggtataaat
     1381 ggccatcagc aatatcatca gatatttgga tacttccacg atcagatttg tcataggcta
     1441 ttctactccc cgtctgaaat gtcctcttgt aaaatctttc actggttcga caatttctct
     1501 tccttctata aaagaagcag caaaagcttt cagtatcttt tgggtttctt cttttccttc
     1561 tcttgtaaaa gaaagacgca gggaagccat gttcagctct cttactgcct cttcattgcc
     1621 aaataaagat aacggaagag gatttaaaat ggtattgtag cagaagttgc aatgtgcctt
     1681 taatggaagc ccggcaccgg tgcggtcctt cattataagg tgttcccttt tggctgtaca
     1741 tccctttaat gtttttacca cacactgggc agaaaccatc ataggagcat gcccataaat
     1801 gatcagttct cccggtattc cgtccctctc acaggctttt gccactgcct ttaactctct
     1861 gctgtttaat tcccacggca tggtaataaa ttccggtccc atttccttta atgtgtgcac
     1921 tgcttcccgg ttccatgcat aaacagaggc atccagagca aatggaagct ctattttttc
     1981 ttcctttaac caggaaactt cctccaaagt acgggccaga acaccggtaa aacctgcatt
     2041 ttccagattt ttcctgtttt tagagaagaa cttcatggca tgggtgcgga aaatatgtgg
     2101 aagtgtaagg aagcattcct ttccctttgc ttcacactgc tttacgatct ttccccactg
     2161 atccggttta aatccatctg catcgatata aatgcgtttt acagcatcac aggaaagagc
     2221 tggttccaac tgacattctt cttccagaga tgcagtaagg aaagcaaagc tgtgaatgtt
     2281 tttggaagca gactgtacat cagcaggaag ttcctgttct gtctgcacca gagactctgc
     2341 ctttctctcc cactgagcca gtattttttc cgtcagcttc tcaaagcctt ctcttcgcag
     2401 ttcatttaat gcctgtaccg gcaggaaaat atttcccttt aactcaatat ccagctgctc
     2461 aaagtaaaag gaagtggcac ctgtcttatt taactgctta cgcacttttt cctctgttgc
     2521 aggctggttc tgggcactct ggcagatctg tccttccact tttacagtta catttccctg
     2581 tgtcagttcc aggacagccg gttttccttc ctcaaaaaca gcctttccaa caaccggttc
     2641 ctttttctct ttttccacat agtttgcatc cagaaaatca aacagtttct gatttccttc
     2701 acgaaattcc ggcttttctt ttaaagcgac catgtctttt ccattgtgct tctcataata
     2761 accggaggta aaacctcccc tgtcaaacag atccagcagt tcttttttat cttccggatc
     2821 cacataataa ccgtctttcc cataggtttc atacagatcc agatattttc tgtaaatgcg
     2881 gactacacct gctgtatatc gcgggctctt cattcttcct tcgatcttta aagaatatac
     2941 tcctgcctct aagatctccg gtaaaatatc cagcgtacac agatctttta aacttaatac
     3001 aaacttctgg ttctctttgt ttaagctctt tccctctgcc ttgtctccct cccatacacg
     3061 gtaaggcagg cggcatggct gggcacagcg gcctctgttg ccactgcggc cgccgatcag
     3121 gctgctcatg agacactgac cggaataaca gtaacataaa gcaccatgga caaaagtttc
     3181 aatatccatt cccgtttctt tgtaaatacg ggaaatctct tctaaagaca gttctctggc
     3241 cggaacgacg cggcagcagc ccatttcttt taacattttt gcgctgtata cactggtaac
     3301 cgtcatctgg gtgcttgcat gaagctccat taccggaaaa gcccgtttca ttacctgaag
     3361 ggctcccaga tcctggacga tcacgccgtc taaccctgct tcatagtaag gcttcatgta
     3421 atcacaaagc tcgtccagtt ctttttcctt aaataatgta ttgaccgtca tgtacagacg
     3481 gcagccgtga agatgggcat accggatggc ttccaccatt tcttctcctg ctgcattctg
     3541 tgcataagct ctggcaccaa aacggctgcc tcccatatag atcgcatccg ctcctgctgc
     3601 cacagctgcc tttaaactgt caaaagaacc tgccggcgcc aaaagctctg cttttctttt
     3661 atttatcatg atcttacctt tcctgatcct atgcaaaaaa ggggccactg caaactgtag
     3721 cgcgccccct ttagtctatc tgtgttttat ctgcgtccgt ttctgtggga accggctttt
     3781 ctggctgcct gaagagcctt tcttgcaagt tcctgatcag aaagtcctgt ctttgtctgg
     3841 aaagaaggat tttgcattgg catatccgcc tgtacaaaac cgctttctgt tgcctgtgca
     3901 gctgcagtct catctaaagc tggtacagac tcactgaccg gttcatctcc ttcgatcaca
     3961 gtgtctacca cttcatcctc aactacttcc tcttccccat tttcagaggt tgctgccagt
     4021 gctgcctgct tctttgcagc tcttaattcc cgttcggttt ttgaaagctt tgcagataat
     4081 tcctgaagct gacgctggcg ttcctccaag tcctttaaaa caccgtctaa cttcatctgg
     4141 gtgcttacta actcatgttt taagctgtaa gtctcttttt ccattctgtc tctctgctct
     4201 tccatctgat ccgcagtttc ttttgtctta aaataatcat ctgcaatgtt cagtaaaagc
     4261 ataagctgct ggtaatcagc actctgttta ctgaaaccag gaagcgcgtg catggtggtt
     4321 atcttttcat ttacatagct ggctgccttc tgaagatatg cttcatcttc gcttccgcct
     4381 aacgtatata tttttccatc aatcaaaacc tgtgtataat tctttgtatc cattctgatc
     4441 ttccatctcc tgtctgtgag gcattttcgt taaaaggata ccacattttt acttcatttc
     4501 caagcccaaa tgacgatatg cgttttctgt agccattctt cccctgggtg ttctgttaat
     4561 cagaccgttc attaaaagat atggctcata tacatcttcc aaagttcccg catcttctcc
     4621 caaagctgct gccagggtat caagtcctac cggaccgccg gaaaactttt caatgatcat
     4681 caaaaggata tttctgtcat tctgatccag tcccatttta tccacatcca tgatatccag
     4741 agcaaaatct gccacttctt ttgtgatcac accatcatat ttaacctggg cgaaatctct
     4801 tactctcttt aaaaggcggt ttgccagtcg cggggttccc ctggaacgtt ttgccagttc
     4861 aatggcacct tccggttcta tttccacacc taagatcgta gcagaatgct ggatgatcgt
     4921 gcgcagctcc tccggcgtat aaaattccat tctctggacc actccaaaac ggtctcttaa
     4981 aggagcggac aacagtcctg ccctggtagt cgctcctact aaggtaaagt gaggcagttc
     5041 cagacggatg gatctggcac tggactcttt tcccagcata atatcaatgg caaaatcttc
     5101 catcgccgga tagagaactt cttctacctg acggtttaaa cggtggatct catctacgaa
     5161 gagaacatcc ccctcctgaa gattatttaa aatagcagcc atctctcccg gcttttcaat
     5221 ggccggcccg ctggtcactt tcagcctgct tcccatctcg ttggcaatga taccggaaag
     5281 ggtggtcttt ccaagtcctg gggggccgta aaataataca tgatctaagg cttcccctct
     5341 gctttttgcc gcttctatgt atattttcag ggtggatttg atcttttcct ggcccacgta
     5401 atcactgagg cactggggac gcagggtggt ttctatccgc acatcctctt ctgttgcctc
     5461 tgttgttata attctccgtt ccatatatac ctcaaattaa tcacagaaaa cttaaatact
     5521 tcagcgaagc ctttaaaatg tcttctacac tcatattttc tgtgatctcc actttcttta
     5581 ctgctttgga agcttccata ttcgtatacc caagagctgt taaagctgcc actgcctctt
     5641 ttgcagctgc tgccatacct gccacagatg cgcctgtacc agcaccggaa gtatcttcct
     5701 ttccgcctcc aaaggaagcc atcatcactt cctctaaatc tactttatcc ttaagatcta
     5761 agatcagtct ctgggccgtc tttgcgccga tgcccggtgc cttggaaata gcttttgcat
     5821 ccccgctgac aatggcgatc cgcagatcat ccggccgcat aatagaaagg ataccaagag
     5881 ctcctttggg accaattcca ttgaccccga tcagctgctt gaacatttcc ctgtcctgtc
     5941 tgtgaagaaa accatataaa gtcatagcat cttcccgcac ctggaaatac gtataaacgg
     6001 tcacttcttc ccctaatgcc ggaagttcct ctaaaagtga aaggggtaca tggactgcca
     6061 gtccgatgcc tccggtctcc actacgatca catcatcttc tactgcccca agggctcctt
     6121 ttatatatga aatcatctgt tctatattcc ttttatctgg tttcgcacct atatgcgata
     6181 cttcgtcatc tatcaatcat aacatacaca ccgcctcaat gcaagcagga caccgaacaa
     6241 atattcgata ctctttcctt gcattttact taaaaatcag ctaaactaag ggcggtttgc
     6301 gttctggtct ttcaggatca gatatgccgg gcagaccaga aaggattttt atgatcacca
     6361 ttaaacatcc cattgaattt ccaaacacat atcctttaga ccgcattggt cctttaaagg
     6421 atcttttatt ttttgatata gagaccaccg gcttttccgg tgaaacttcc cagctgtatc
     6481 tcattggctg tacttatttt gacggatttg gctggaagct gatccagtgg tttgcagata
     6541 caagacaggc ggaaaaagag cttttagatg ccttttttga atttttaaaa cgttttaaga
     6601 tcctgatcca ttttaacgga gaccgttttg atatccccta tcttttaaaa tgctgcgaac
     6661 gtctgggcct tgactaccat tttgatgacc ttataagcat tgatattttc aagaaaataa
     6721 agccctacag aaaactttta aaactggaaa acatgaaaca gaaatccatt gaacagtttt
     6781 taggggtaca tagagaggac aaatacaatg gcgggcagct gatcgatgtg taccgtgaat
     6841 acctgcacac ccatgagaaa ttcctctttg acctgctgat actgcacaat gcagatgacc
     6901 tgcggggaat gccttccatc ctgcccattt taaattatgc agatctgttt gagtcggatt
     6961 ttaccctttg cagtcataag ttgtattcct acacggacga agccggatat tcccatccat
     7021 ttgtcaatct tttgtgggaa agtccttaca gcgtccccat tcctttagaa tataccttaa
     7081 atcctttaag cctggagctt catgacaact gcctggcctg caatttaact ctgtatgaag
     7141 gcactttaaa atatttctac ccggattacc agaattatta ttacctgcct ttagaagata
     7201 cagcgatcca taaaagcatt ggagaatatg tggaccgttc tgcccggaaa aaagccactg
     7261 caaaaaactg ctacagcaaa aaagagggct tattcctgcc tcagttcggt tctttgtggg
     7321 aaccttcttt taaggaagaa tacaagtcct ctctcactta cacgctttat gatgataaaa
     7381 ccctgtggaa tgataccgtg aaaaattctc actatgccca tgagatcttt aactatctga
     7441 ccgggcagct gtagctgccg catacacagc atacatctgg gaagcgccgg tatccccatg
     7501 tcctaacata gcctggactg catggatatc cgcgccatcc ccgatcaggt gggctgcaaa
     7561 agaatggcgc agagtatgag gcgtaatatc ctctttgatc cccgtttttt ccccataata
     7621 tttgatcagc ttccaaaagc cctgacggct cataggctgg ccactgcaat tggtaaacag
     7681 ccactcacag gcacgcccct ttaaaagaga acttcttgcc ttgtccaggt aagtacgcag
     7741 cgcctgtttc gccccttttc caaaggggat catccggtct ttactgccat catgacaggt
     7801 gatataaccg atctgcatgt tcacatcctc taatttcaga ctgatcagtt ctgatacccg
     7861 gatccctgtt gcatatagta attccagcat ggccttatcc cgcagttctt tcgctgttgt
     7921 atttcccggc tgtgccaaaa gggcatgtat ttcctctatg gtcagtatca ccggtatttt
     7981 gcgttcaatt ttgggagcac gcagaaattc cgccggatcc ctgtttatct ttctttcctt
     8041 aacaagatag ctgaaaaatg ccttgatgga cgccagttcc ctggaaatgg tagctgccgc
     8101 ttttccttct ttttccagat acaggatata agaattcaaa ctggtcttcg tcaccttctc
     8161 cacctgtgtg atccccattt cttccataaa tcctgccatc tggagcagat cccggtggta
     8221 ggatacttct gtattttttg ccgtgtgctt cacttcctga agatatgcgg tgaagctgtt
     8281 tatctctgat atcattttgt tcc
//
LOCUS       DBMDUA010000122         8302 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3868_length_8302_cov_2.684551, whole genome shotgun sequence.
ACCESSION   DBMDUA010000122 DBMDUA010000000
VERSION     DBMDUA010000122.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8302)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8302)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8302
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3868_length_8302_cov_2.684551"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(399..1676)
                     /gene="spoIVB"
                     /locus_tag="ACZPCL_09390"
     CDS             complement(399..1676)
                     /gene="spoIVB"
                     /locus_tag="ACZPCL_09390"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HIR1162715.1"
                     /translation="MRGKSNFYKHLVRIFWLSLIVGTVGIWYYVDRSIPDRVSVTEHD
                     QEEFTFSLPWRATVYSESEEVALGNKSNIPAEQITINSREPFSMYAGKKGSYQLDLKL
                     FGVIQFKNIQVDVVDTTRLIPCGSPVGIYLKSKGIMVIGTGRITGENGMEMEPAVGKL
                     KSGDYIEAFNGQKLGTKEELAEAVSHFQGENVTLTVRRNGAETQVKIKPVKGTDGSSK
                     LGVWVRDDTQGIGTVTYMDLNGKFGALGHGISDSDTGELVETSGGNLYDTQILGIEKG
                     KIGTPGMLSGVIYYGPKSEIGTVTENTDYGIFGTVSQTFLKEHRTASMEAARRQEVKT
                     GPALVRTSISGQVKDYSVEIQRIDMTGNKKNKSLVIKITDPELLNLTGGIIQGTSGSP
                     VIQNGKIIGAITHVFVQDPTRGYGILIEDMLGH"
     gene            complement(1855..3513)
                     /gene="recN"
                     /locus_tag="ACZPCL_09395"
     CDS             complement(1855..3513)
                     /gene="recN"
                     /locus_tag="ACZPCL_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR1162716.1"
                     /translation="MLLELHVKNLALIEKADVEFGEGLNILTGETGAGKSIIIGSVTM
                     ALGGKASKDVIRRGADHAYIELIFFVDSKEKKKALMQLGTEPTEEGMVIISRKIMPSR
                     SVSRINDETVTMARLRQITGLLLDIHGQHEHQSLLYKSKHLEILDAYVKAQTQPVKRK
                     IEEEYKNYCKLKERLLSFKTDEDARLREMDFLRFEIQEIENGEVKEGEEEELTLKYRR
                     FAHARRIAEELENARGLLQRDFAAQALRSVEQAAEYDKALEGIRDQLYDAEAILNDAS
                     REISSYMGSMEFDEEVFRTTESRLDQIHDLQRKYGNTTQLMLENLEKKKNRLEELENF
                     DAYREKTEKELKEAQDRLERCCAELSKIRKTASRQLVEKIKKGLLDLNFLDVQFDMEF
                     DRLPHFTASGYDEAQFLISTNPGQPVRPLMEVASGGELSRIMLAIKTVLADSDDIPTL
                     IFDEIDTGISGRTAQKVSEKLSYIAKSHQVICITHLPQIAAMADTHFEIHKAASEGST
                     ATSIRRLNREQTVEELARLLGGAKITDTVIQNAREMKELADKTK"
     gene            complement(3566..4018)
                     /gene="argR"
                     /locus_tag="ACZPCL_09400"
     CDS             complement(3566..4018)
                     /gene="argR"
                     /locus_tag="ACZPCL_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIR1162717.1"
                     /translation="MKLERHSKIVELIGKYEIETQEELAEKLTQAGFNVTQATVSRDI
                     RELKLTKVPGENGRQRYMVLESPRGTSASKYIRILKDGFVSMDMAQNILVIKTVSGMA
                     MAVAAALDAIKFHEVVGCIAGDDTIMCAVRSVDDTIIVMEKIKKMVED"
     gene            complement(4049..4888)
                     /locus_tag="ACZPCL_09405"
     CDS             complement(4049..4888)
                     /locus_tag="ACZPCL_09405"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR1162718.1"
                     /translation="MKYFYVISNLSKDYVLDVQDEVQSCLEKRGAVCRYMTDYERMKN
                     GRHTPGEYVPEDTQCIITIGGDGTLIQAARDLAGRNIPMLGINRGHLGFLNQVDRTED
                     IDAAMDALVNERFRLEHRMMLRGTVFHEGKPVFQDIALNEIIATSAEHLRVLRYDLAV
                     NDEYLNEYAADGVMVATPTGSTAYNLSAGGPVMAPAGRMMAVTPVCSHTLNARSIVIS
                     EEDRVKIGLLGKGLVTFDGDTSQKTGPGDYLIVERSHMETVMVKLKQISFMQNLSNYL
                     GNV"
     gene            complement(4909..5733)
                     /locus_tag="ACZPCL_09410"
     CDS             complement(4909..5733)
                     /locus_tag="ACZPCL_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR1162719.1"
                     /translation="MKERLDVLLVKRNLADSREKAKALIMSGIVYVNGQKEDKAGTTF
                     DETAPIEVRGNTLKYVSRGGLKLEKAMTHFGVELKDKICMDVGASTGGFTDCMLQNGA
                     TKVYSVDVGHGQLAWKLRNDERVVCMEKTNIRYVTHENIPDEIGFSSIDVSFISLTKV
                     LGPVKALLKDDGQVVCLIKPQFEAGREKVGKKGVVRDKAVHLEVIEMVIAFAKSIGFE
                     ILNLEFSPIKGPEGNIEYLLHLQNHPEGEDGLVIYEEMPVDPKKIVEEAHAALDKE"
     gene            complement(5773..7635)
                     /gene="dxs"
                     /locus_tag="ACZPCL_09415"
     CDS             complement(5773..7635)
                     /gene="dxs"
                     /locus_tag="ACZPCL_09415"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR1162720.1"
                     /translation="MILEQIKGPEDLKKLTSEELSQLAEEIRAFLIEKISHTGGHLAS
                     NLGVVELTIALFRTFDLPEDKIIWDVGHQSYTHKILSGRRMEFDELRQYGGLSGFPKR
                     KESPYDSFDTGHSSTSISAGLGIAQGRDILGEDYKVVSVIGDGALTGGMAYEALNNAA
                     RMKKNFIIILNDNKMSISENVGGMSRYLGGLRTGAGYNDLKKNVADTLERIPVVGGRM
                     IDKIKRTKNSIKQLFIPGMLFENMGITYLGPVDGHNIPALCKVLKEAQKLDHAVLVHV
                     LTKKGKGYEPAEKNPAHFHGVSPFDIKTGKPLAEKKYPTYTDVFSKKLCQLGETHPEL
                     VAVTAAMPDGTGVAAFGKKFPDRFFDVGIAEAHAVTSAAGMAAAGLRPVVAVYSSFLQ
                     RGYDQILHDVCIQNLPVLFAVDRAGLVGSDGETHQGIFDYSYLTSIPNMSVAAPKNLW
                     ELRAMLDFAMDYKAPFAIRYPRGTAYRGLKEFMQPIAYGKGEMIYEEENIALLAVGSM
                     VSTGEHVRAKLKEEGVSCTLANARFVKPFDKELVDRLAKNHRLIVTMEENVLQGGFGL
                     PVTAYIHEHYPQVKVMNIALPDAYVEHGNVSVLRKGLGIDSDSIIQRLKAEGWL"
     gene            complement(7649..>8302)
                     /locus_tag="ACZPCL_09420"
     CDS             complement(7649..>8302)
                     /locus_tag="ACZPCL_09420"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR1162721.1"
                     /translation="VHDDLPCMDDDMMRRGQASTWAEYGEDMGVLAGDALMIYAFEVA
                     AKAFKEVSDADDLKRVGRAIEILASKTGIYGMIGGQTVDVELAGGPIPKNKLDFIYRL
                     KTGALIEASMLIGAVLGGAAEEDCKIVESLAGKVGLAFQIQDDILDVTGDQEVLGKEA
                     GSDEKNQKTTYVTLEGLEKAKKDVEALSVQAIEDLNKLPGNNEFLENLIRILIKRQK"
BASE COUNT         1978 a         2187 c         1551 g         2586 t
ORIGIN      
        1 catgtcaatg gcgtcttttc cattatcagc tctgccaatt acagaaagtc ctgcttcaga
       61 actgatcact tcatctaacg tgtttaagat cataggatta tcatctacaa ttacaacatt
      121 tacattttcc atgtttctac ctcctgcatc ccttaacgca atttcattct ttacttatag
      181 agtgcttcct cattatttaa cgctttgatg cgttaagtct tttatggtag atattataaa
      241 tgcaaatatc gtcatatttc aagaaaaagc caccgcatta ttagacattt taacatttta
      301 ttaacagtta tcttttatta tctaatattg tcgaattttg tcaattttta ttagtttgtg
      361 actttattca ctgttttgca taaaattatg gatccttatc agtgccccag catatcctcg
      421 atcaatatcc catatcctct tgtaggatcc tggacaaata cgtgggtgat ggccccgatg
      481 atctttccgt tttgtattac gggggaaccg ctggttccct ggataatgcc gccggtgaga
      541 tttaacagct ccggatctgt gattttgatc accaggcttt tgtttttctt gtttcctgtc
      601 atatcgatcc gctgtatctc aacagaataa tctttcacct gccctgagat gctggtacgc
      661 accagggcag gtcctgtttt tacttcctgg cgcctggctg cttccatgga ggcggttctg
      721 tgttctttta agaaagtctg gcttacagtt ccaaatatcc cataatctgt attttctgtc
      781 actgtcccga tctccgactt tggaccatag tagatcaccc cggaaagcat tcccggcgtt
      841 cctatttttc ctttttcaat tcccaaaatc tgggtatcat aaagatttcc tccggaagtc
      901 tccaccagtt cccctgtatc gctgtcactg atcccatggc ccaatgcacc aaattttcca
      961 tttagatcca tataagtcac agttccgatc ccctgggtat catcccgcac ccacacacct
     1021 aatttggagg aaccgtctgt tccttttaca ggctttatct taacttgtgt ttctgcccca
     1081 tttctgcgca ctgtcaatgt aacattttct ccctgaaaat ggctgaccgc ctctgccagc
     1141 tcttcctttg tcccaagctt ttgcccatta aaggcttcaa tatagtcccc ggattttaat
     1201 tttcccacag ccggttccat ttccatccca ttttccccgg taatacggcc ggtaccgatg
     1261 accataattc ccttagactt taaataaatt cccacaggac tgccacaggg gatcagcctg
     1321 gtagtatcta ccacatctac ctgtatgttc ttaaactgga tcacaccaaa taatttcagg
     1381 tccagctgat aactgccttt ctttcctgca tacatggaaa atggctctct gctgtttatg
     1441 gtgatctgct cggccgggat attgctctta tttcccagtg ccacttcttc gctttcactg
     1501 tatacagtag cccgccaggg aagggaaaat gtaaattctt cctgatcatg ttctgttacg
     1561 ctgacccggt ccggtatgct ccggtccaca tagtaccaga tcccaactgt acccacgatc
     1621 aaggacagcc agaatatcct gaccagatgc ttataaaaat ttgattttcc cctcatagaa
     1681 cgacctcctt ctccatttgc agcctgcaac atgcgccatc ggtgcatttt ctctgtacaa
     1741 aaacagggag ccgcaagtcc tgatactctt gcgctccctg ttagtatgtt ctgtgaacgg
     1801 tcgttccaat ctgttttatt ttggttattg cttatgcctt gcacggacag ttgcttattt
     1861 tgtcttatct gccaattcct tcatttcacg ggcattctgg atcactgtat ctgtgatctt
     1921 tgctccccca agaagtcttg ccagttcttc cactgtctgc tccctgttaa ggcgccggat
     1981 ggaagtcgct gtacttcctt cactggctgc tttatggatc tcaaaatggg tatctgccat
     2041 cgccgcgatc tgaggcagat gggtgataca gatcacctga tggctctttg caatatagga
     2101 aagtttctcg gaaaccttct gggctgtcct tccgctgata cccgtatcga tctcatcaaa
     2161 aataagggtt gggatatcat cggagtctgc aagaacggtt ttgatcgcca gcatgatacg
     2221 tgacaattca ccgccggagg ccacttccat aagagggcgc accggctgtc ccggattggt
     2281 agagatcaaa aactgggctt cgtcatagcc ggaggctgta aaatgaggca gccgatcaaa
     2341 ttccatatca aactgaacat ctaaaaaatt cagatccagc agtccttttt tgatcttttc
     2401 caccagctgt ctggacgcag tttttcgtat cttggataat tctgcgcagc atctttctaa
     2461 acgatcctgg gcttctttta actctttttc cgttttttcc ctgtaagcgt caaaattttc
     2521 cagttcttcc agcctgtttt tcttcttttc cagattttca agcatcagct gcgtggtatt
     2581 tccatacttt ctctgaagat catggatctg atcaaggcgg ctttctgtgg tgcggaatac
     2641 ctcttcatca aattccatgg aacccatata agaagagatc tccctggaag catcatttaa
     2701 aatagcttcc gcatcataaa gctggtctct tatcccctct aatgccttat cgtactcagc
     2761 tgcctgttct accgagcgca gggcctgagc tgcaaagtca cgctgcagca gtcctctggc
     2821 attttccagt tcctctgcga tccttcttgc atgggcgaaa cggcggtatt ttaaggtaag
     2881 ttcttcctct tctccctctt ttacctctcc gttttcgatc tcctggatct caaagcgcag
     2941 aaaatccatc tcccgcagtc ttgcgtcctc atctgtctta aaagataaaa gacgttcctt
     3001 taacttgcag tagtttttgt actcctcttc gattttcctt ttcaccggct gggtctgtgc
     3061 cttgacatag gcatccagga tctcaagatg ttttgactta tacagcagag actgatgctc
     3121 atgctgacca tggatatcta ataaaagacc tgtgatctga cgaagtcttg ccattgtcac
     3181 agtctcatca ttaatacggc tgacacttct ggaaggcatg atctttctgg aaatgatcac
     3241 cattccttct tctgttggct ctgtcccaag ctgcattaac gcctttttct tttccttact
     3301 gtcaacgaaa aagatcagct caatatatgc atggtccgct ccacggcgta tcacatcctt
     3361 tgacgctttt ccccctaacg ccatggtcac ggaaccaata atgatcgatt tacctgcacc
     3421 ggtctcgccg gtaagtatat tcagtccttc tccaaattct acatctgcct tctcaatgag
     3481 agccagattt ttcacatgga gctcaagaag catgctattc ctctctttct atcttttttc
     3541 ccggatcttt tgttctaaat ccagtttaat cttccaccat ttttttgatc ttttccatta
     3601 cgataatggt atcatccaca ctgcggactg cgcacataat ggtatcatct ccggcaatac
     3661 agcctactac ttcatggaat ttaatagcat caagagcagc tgcaactgcc attgccatac
     3721 cggaaactgt tttgatcaca aggatattct gtgccatatc catggataca aaaccgtctt
     3781 ttaaaatgcg gatatatttg gaagcagaag ttcctcttgg actttcaagg accatatagc
     3841 gctgacgtcc attttctccc ggcactttcg tcagttttaa ttcccggata tccctggata
     3901 cagttgcctg ggttacatta aaacccgcct gggtcagctt ttcagccagc tcctcctggg
     3961 tctctatttc atattttccg atcagttcta cgatcttgct gtgacgttcc aatttcatct
     4021 gcttttcctc ccatctgttt ccacatcttc agacatttcc cagataatta ctcagatttt
     4081 gcataaaaga gatctgcttt aattttacca ttaccgtttc catatgtgag cgctctacga
     4141 tcagataatc cccgggacct gtcttctggg aagtatctcc gtcaaaagta accagtcctt
     4201 ttcccaaaag cccgatcttt acacggtcct cttcggaaat gacgatactt ctggcattta
     4261 aagtatggga acatacaggt gttactgcca tcatccggcc tgcaggagcc ataaccggcc
     4321 ctcctgcgga aagattatag gcagtagaac cggtaggggt tgccaccatc actccatccg
     4381 ctgcatactc atttaaatat tcatcattta cagccagatc atagcgcagt acccgcagat
     4441 gctctgcact ggtggctatg atctcattta aagcaatatc ctgaaatacc ggctttccct
     4501 catgaaatac agttccccga agcatcatac gatgttctaa acggaaacgt tcatttacaa
     4561 gggcatccat cgcagcatca atatcctctg tgcggtccac ctggtttaaa aagcccaaat
     4621 gtcccctgtt gatcccaagc atggggatat tccggcctgc cagatctctt gctgcctgga
     4681 tcaaggtacc gtcgccgcca atggtgatga tgcactgggt atcttccggc acatattcac
     4741 cgggcgtatg tctgccgttt ttcatacgct cataatctgt catataacgg catacagccc
     4801 ctcttttttc caggcaggac tgaacctcat cctgaacatc cagtacatag tctttactta
     4861 aattggatat tacataaaaa tatttcataa gtcccctatg ctaaaacctt attctttatc
     4921 cagcgctgcg tgtgcttcct ctacgatctt tttcggatca acaggcattt cctcgtaaat
     4981 gaccagtccg tcttcgccct ccgggtggtt ctgcagatgc agcagatact caatatttcc
     5041 ttccggtccc ttgatcggtg aaaattccag gtttaagatc tcaaaaccaa tggattttgc
     5101 aaaggcaatg accatttcaa taacctctaa atgaacagcc ttatccctta caacgccttt
     5161 tttgccaact ttttctcttc ctgcctcaaa ctgtggcttg atcagacata ctacctgacc
     5221 gtcatccttt aaaagagcct ttacaggtcc aagcaccttg gtcagagaga taaaagatac
     5281 gtcaatgctg gaaaatccga tctcatccgg gatattttca tgggtcacat agcggatatt
     5341 agtcttttcc atgcagacta cacgctcatc attgcgcagc ttccacgcaa gctgtccatg
     5401 gcctacatcg acagaataaa ccttggtagc gccattttgc agcatacagt ctgtaaatcc
     5461 gccggtagaa gcgcctacgt ccatacagat cttatctttt aactctacgc cgaaatgggt
     5521 cattgccttc tctaatttca ggccgccgcg gcttacatat tttaatgtat ttcctctcac
     5581 ctcaataggt gctgtttcat caaaggtggt acctgcttta tcctctttct gtccgtttac
     5641 ataaacgata ccggacatga tcagagcctt tgccttttct ctggagtctg ccagattgcg
     5701 ctttacaagc agtacatcta accgttcctt catttatttt ctccttgtat ctatcttatc
     5761 gatctttctg ttttagagcc atccttctgc ttttaacctc tggatgatag agtcactgtc
     5821 gatccccagt ccttttctta aaacagacac gtttccgtgc tcaacatagg catcaggaag
     5881 tgcaatattc atcactttta cctgcggata atgctcatgg atataggcag ttacaggaag
     5941 tccaaaacct ccctggagca cattttcttc catggtcacg atcagtctgt ggttctttgc
     6001 cagacggtct accagttctt tatcaaaagg ttttacaaaa cgggcatttg ccagtgtaca
     6061 ggaaactccc tcttctttta actttgcccg tacatgttct ccggtactta ccatacttcc
     6121 cactgctaaa agggcaatgt tttcttcctc atagatcatt tccccttttc cataagcaat
     6181 aggctgcata aattctttta agcctctgta agcggtacct cttggatagc ggatagcaaa
     6241 aggtgcctta taatccatgg caaaatccag catagcccgc agttcccaga gatttttcgg
     6301 cgctgccacg ctcatgtttg gaatagaagt aagataagaa taatcgaaaa ttccctggtg
     6361 ggtctcccca tcgcttccta ccagtcccgc acggtccact gcaaagagca ccggcaggtt
     6421 ctggatacag acatcatgta agatctggtc ataacctctc tgtaaaaagg aggagtacac
     6481 cgcaactaca ggccgcagtc ctgctgccgc cataccggct gcgctggtca ctgcatgagc
     6541 ttccgcaata cctacatcaa agaaacggtc cgggaatttc tttccaaaag ctgccactcc
     6601 ggtaccgtca ggcatggccg cagtcacagc taccagctcc ggatgtgttt ctcctaactg
     6661 gcacagtttc ttggaaaata catctgtata agtagggtat ttcttttctg ccaaaggttt
     6721 tcctgttttt atatcaaaag gacttacacc gtgaaaatga gccgggttct tctctgccgg
     6781 ttcatagcct tttccttttt ttgtaaggac atggaccagt actgcatgat ccagtttctg
     6841 ggcttctttt aataccttgc acagtgcagg tatattgtgt ccgtccactg gtcccaaata
     6901 ggtgatcccc atattttcaa agagcattcc cgggataaac agctgtttga tactgttttt
     6961 cgtccgtttg atcttgtcga tcatacggcc gccaactacc gggatccgtt ctaaggtatc
     7021 tgccacattt ttcttcagat cattgtaacc ggctcctgtg cgaagtcctc ccagatacct
     7081 tgacatgcca cctacatttt cagaaatgga cattttattg tcatttagta tgataataaa
     7141 gtttttcttc atacgggccg cattatttaa agcctcgtaa gccattcctc ctgtaagagc
     7201 accatcacca atgacagaga ctaccttata gtcctctcct aaaatatcac ggccctgggc
     7261 aatgccaaga cctgctgaaa tagaagtgga actatggcct gtgtcaaagg aatcataggg
     7321 gctttccttc cgttttggaa agccgcttaa gccgccgtac tgacggagtt catcaaattc
     7381 catcctgcgg ccgcttaaga tcttgtgggt gtaggactga tggcctacat cccagatgat
     7441 cttatcttct ggcaggtcaa aggtacgaaa cagagcaatg gtcagctcca caactcccag
     7501 gttggaagct aaatgtcccc ctgtatggct gatcttttcg atcagaaagg cccgtatctc
     7561 ttctgccagt tgggaaagtt cttcagaggt caattttttc agatcttccg gcccctttat
     7621 ctgttccaat atcataggtt cttccttttt atttctgacg tttaataaga atacggatca
     7681 aattctccag gaattcatta tttccaggca gtttgtttaa atcttcaatc gcctggacag
     7741 aaagagcttc tacatctttc tttgcctttt ctaaaccttc cagagtcaca taggtagtct
     7801 tctggttctt ttcgtcgctt cctgcctctt tgcctaaaac ttcctggtct ccggtcacat
     7861 ccagaatatc atcctggatc tggaatgcca gaccaacctt gcctgcaagg ctttctacga
     7921 tcttacagtc ttcttctgca gcgccgccta aaacagcacc gatgagcatg gatgcctcaa
     7981 taagggcacc tgtctttaag cggtagataa agtccagttt attcttcggg atcggtccgc
     8041 cagccagttc tacgtctact gtctgtccgc cgatcatgcc gtaaataccg gtcttggaag
     8101 ccaggatctc aatggcacgt cccactcttt taaggtcatc tgcatctgaa acctctttaa
     8161 atgccttcgc tgctacctca aaagcataga tcatcagcgc atctcctgcc agaacgccca
     8221 tatcttcgcc gtattcagcc caggtacttg cctgtcccct gcgcatcata tcatcatcca
     8281 tacatggaag atcatcatgt ac
//
LOCUS       DBMDUA010000123         8263 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3883_length_8263_cov_1.938373, whole genome shotgun sequence.
ACCESSION   DBMDUA010000123 DBMDUA010000000
VERSION     DBMDUA010000123.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8263)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8263
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3883_length_8263_cov_1.938373"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="ACZPCL_09425"
     CDS             complement(<1..1274)
                     /locus_tag="ACZPCL_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR1162722.1"
                     /translation="MKKKKKWIKLVIILLVIAIAARAIFVWKSHKAKAAAAAATAENT
                     ATVEKRSISSQLSSSGTLEAKDTYSITSMVEGEVVSANFEEGDQVEKDQVLYEIDRSS
                     MDTDLNSAQNSVTRSQTSYADALDDYNQAASDYSGNTYKATDTGYIKELYISVGDKVS
                     GNTKLADIYSDDLMEIRIPFLSGETELIPVGSTAVLTLVDSGEQIEGTVKAVANREET
                     LSGGRLVKYVTITVNNPGGLTTSTVASAQIGEFVGSEEGTFKASTDTTMNADLAVSVE
                     VEELLVHEGDYVTKGTPIFRMTSRTAEKLMRNYKDALDKAQESVESAQSKLESTQDSY
                     DNYTITAPISGQVITKNFKVGDNITKNTSSTTTLATIYDLSALTFKMSIDELDIQSVK
                     VGQNVTVTADAFEGQTFSGTVTNVSLESTYSNG"
     gene            complement(1285..2457)
                     /locus_tag="ACZPCL_09430"
     CDS             complement(1285..2457)
                     /locus_tag="ACZPCL_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR1162723.1"
                     /translation="MKVRKNHTRFLCGSLAAAAAISPILSITAWADNISTANFNLRQQ
                     VVKLTGIMEISSFRESVTRADFAKMLVKASSYRENLPTSNVSVYADVPATDPNAVYIR
                     IAAREGWMSGYLGGKFKPEDPVLYKDAVKAILTMLGYTDDDFTGDLVSSRISKFNYLE
                     LNEDVSRQAADEVNQTDCMNIFYNLLKTKKKDSNEIYGTILDCELNSDGEINPITILD
                     DERKGPILVHKNFSVSQSVPFDTEDANVFLNGVASTLSAVKSAQQQAGFAVLYYNVKS
                     KTIWAYTTMGWDNDDNSGNNSYILLKGEIKNIYYKSTDVMTPTSVRIEVDQANSDDSF
                     DTSEDVDSDGYLTISLDSSELQYMFSIYGDLEVGDDVVLVCNRNGSSYTAVDALEY"
     gene            complement(2677..4245)
                     /gene="cls"
                     /locus_tag="ACZPCL_09435"
     CDS             complement(2677..4245)
                     /gene="cls"
                     /locus_tag="ACZPCL_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIR1162724.1"
                     /translation="MKSQPRPRSLFRIIFGRTTFVILGLLLQIAFFFSIFRWLGNYIH
                     FVYITYVLVSSSVVIAILNRPMDSSFKMAWIIPVLLIPIFGIILYVFVQVQFQTRALA
                     RRVNLSVEKTKPYLVQDPQVQKRLRAASVRGSRLVDYMNHIAGFPVYENTAAKFFPLG
                     EDMFEQLMEELKAAKHFIFMEYFIIDRGYMWDSILEVLRQKAAEGVEVRVMYDGMCCL
                     MLLPYHYPQKLENMGIRCQMFSPIKPVLSTHQNYRDHRKVTVIDGHTAFTGGVNLADE
                     YINRKERFGHWKDTAIMIKGDAVKSFTMMFLQMWDAASSKSKNEENYEQYLVPADYKL
                     PEVYKADGFVMPYADSPLDEEQVGEQVYLDILNQAKSYVHIITPYLILDDGMKNAMAY
                     AAKRGIDVKIIMPHIPDKKYAYLLARTYYPELIASGVKIYEYEPGFTHAKIFVSDDEK
                     AAVGSINLDFRSLYLHFECGTYLYRNSTVADVEKDFENTLEKCIPVTLKDCAQYNWFG
                     TKAGRFLRLIAPLM"
     gene            complement(4497..5837)
                     /locus_tag="ACZPCL_09440"
     CDS             complement(4497..5837)
                     /locus_tag="ACZPCL_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIR1162725.1"
                     /translation="MKENANCSISNIYKLDGRVPIGKAIPFGLQHILAMFVSNLAPIT
                     MIAGAAKVPVTGAELGMLLQNAMFAAGIATMIQLYPLWKIGSRLPVVMGVSFTFVTVL
                     STISANYGYPAVIGAVLIGGLFEGTLGLSAKYWRKIISPIVAASVVTAIGFSLFTVGA
                     RSFGGGYATDFGSVSNLLLGLITLATCLLWNMFAKGYLKQLSVLAGLIVGYIVAIFMG
                     KVDLSMIMSGGIVALPHLLPIKPEFHAGAIMSACIIFLVSAAETIGDTSALVAGGLGR
                     EITGEEISGSLACDGYGSFISGLFGCPPVTSFSQNVGLVAMTKVVNRFTIMTGAVAMI
                     LAGLFPPIGNFFASLPQSVLGGCTIMMFGTILTSGMQMIANCGFTQRNITIVAMALSI
                     GIGFTTTSESGIWAEFSPVVQSVFSANVVAVVFVVAIIMNLILPKNMEVEKLDK"
     gene            complement(5867..6355)
                     /gene="xdhC"
                     /locus_tag="ACZPCL_09445"
     CDS             complement(5867..6355)
                     /gene="xdhC"
                     /locus_tag="ACZPCL_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002197 - xanthine dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase subunit XdhC"
                     /protein_id="HIR1162726.1"
                     /translation="MLKIIHMTVNGKETELAVDERESLLDTLRNRLGLTSVKKGCEVG
                     ECGACTVLVNGEAIDSCIYLTMWADGKSVMTVEGLKGPNGELSPIQRAFIEEAAVQCG
                     FCTPGLIMSAVEIVGTGKKYNREELKKLISGHLCRCTGYENILNAMERIVEEVYKVSH
                     KE"
     gene            complement(6348..7238)
                     /gene="xdhB"
                     /locus_tag="ACZPCL_09450"
     CDS             complement(6348..7238)
                     /gene="xdhB"
                     /locus_tag="ACZPCL_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002197 - xanthine dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase subunit XdhB"
                     /protein_id="HIR1162727.1"
                     /translation="MFDIKSFYEAKDVKDAVEALVREPEAEVISGGTDVLIRVREGKD
                     AGRALVSIHNIKELKGVKVLENGDLWIGAATAFSHITNDPTIQKLVPMLGEAVDMVGG
                     PQIRNTGTIGGNICNGATSADSAASMWTLNALIQLEGPEGHREVPIHEFYTGPGRTVR
                     DRCEVCTGFIIKKEDYEGWYGQYIKYGKRKAMEIATLGCAVRVKLSADKKTIEDVRLG
                     YGVAGPTPLRCHAAEEYLRGKAVSDKEAAAEFAKLALTEVNPRSSWRASKEFRLQLIE
                     ELAKRGLAEAIRRAGGEEDA"
     gene            complement(7253..>8263)
                     /locus_tag="ACZPCL_09455"
     CDS             complement(7253..>8263)
                     /locus_tag="ACZPCL_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR1162728.1"
                     /translation="SLETSSVRMVLNQDGSMQVSMGATEIGQGADTVFSQMASETTGI
                     SFDKVFIVSTQDTDLTPFDTGAYASRQTYVSGMACKKCATELRHRILDYAAYMLNHDV
                     TDLSKTVYADLVKESAGTLREALGLHPEDEVKEDMLHIVDSKIVVHGGDPVLFDVAVV
                     ADTAFYSLDKSIHLTAEVTNQCLDNTFSSGCCFVDVEVDMPLGLVTVKDIINVHDSGI
                     LINPKTAEAQVHGGMSMSLGFGLSEELLVDEKTGRPLNNNLLDYKIPTAMDAPDLHVE
                     FIQLDDPTGPYGNKSLGEPPAIPVAPAVRNAILNATGVHMNVSPMTAQRLIEKFKEKG
                     LI"
BASE COUNT         2140 a         2035 c         1640 g         2448 t
ORIGIN      
        1 ccattagagt atgtactttc cagactgaca tttgtaactg ttcctgagaa ggtctggccc
       61 tcaaaggcat ctgctgttac agttacattc tggcccacct ttacactctg gatatccagt
      121 tcatcaatgg acattttaaa tgtcagtgca gacaggtcat aaatagttgc cagtgtagtg
      181 gtagaactgg tattttttgt aatgttatct cctaccttaa agttctttgt gatcacctgt
      241 ccggagatcg gtgctgtgat ggtataatta tcgtagctgt cctgggtgct ttccaacttg
      301 ctctgggcag actctacact ttcctgggcc ttgtctaaag catccttata attgcgcatc
      361 agtttttctg ctgtacggga agtcatacgg aaaataggag ttcccttggt cacataatcg
      421 ccctcatgga ccagcagttc ttctacttct acgcttacag ccagatctgc gttcatagta
      481 gtatctgtgg aagccttaaa agtaccctct tcactgccta caaactcacc gatctgggca
      541 gaagctactg ttgaagtggt aagtccgcct ggattattta cagtgatcgt cacatatttt
      601 accagacggc cgccggaaag agtctcttcc ctgtttgcaa ctgccttaac ggtaccttcg
      661 atctgctccc cggaatcaac cagtgtaaga acagctgtgc tgcctacagg gatcagttct
      721 gtttccccgc ttaaaaatgg aatgcggatc tccatcagat catcactgta gatgtctgcc
      781 agctttgtat ttccgctgac tttatctccc acgctgatat acagttcctt aatatagcct
      841 gtatcagttg ccttatatgt atttccgctg taatccgatg ctgcctggtt ataatcatcc
      901 agtgcgtctg cataagaggt ctgggaccgg gtaacagaat tctgggcact gtttaagtct
      961 gtatccatac tggatcggtc gatctcataa aggacctgat ccttctccac ctggtcacct
     1021 tcctcaaagt tggcagatac tacctcacct tccaccatgg aagtgatgct gtaagtatcc
     1081 tttgcctcca atgtgccgga agaagacagc tgggaactga tgcttctttt ttctacggtt
     1141 gctgtatttt ctgctgttgc agctgccgct gctgccttag ccttatgact tttccataca
     1201 aagatagctc ttgctgcaat ggcgatcaca agaaggatga tcaccagttt gatccacttt
     1261 ttcttctttt tcataatatc ccccttagta ttcaagtgca tcaaccgctg tataagagct
     1321 tccgttgcgg ttacatacca ggactacgtc atcgcctact tccagatctc cataaataga
     1381 gaacatatac tggagttccg aagagtcaag gctgatggtc aggtaaccat cagagtctac
     1441 atcctcgctg gtatcaaagc tgtcatctga atttgcctga tctacttcaa tacgaacaga
     1501 agttggtgtc ataacatccg tggatttata ataaatattt ttgatctcac cctttaagag
     1561 gatataagag ttattgccgg aattatcatc attgtcccag cccatggtgg tataagccca
     1621 gatcgtcttt gattttacat tgtaatataa aactgcaaaa ccagcctgct gctgggcgga
     1681 ttttactgca gataaagtac ttgccacacc atttaagaac acatttgcgt cttctgtatc
     1741 aaaaggtaca gactgggaaa cgctgaagtt tttatgtacc agaatcgggc ctttccgctc
     1801 atcatccaga atagtgatgg gattgatctc accgtcagaa tttaactcac agtctaagat
     1861 cgtaccatag atctcattgc tgtccttttt cttggtcttt aacaggttat aaaaaatatt
     1921 catgcagtct gtctggttca cctcatcggc agcctgacgg cttacatcct cgtttaattc
     1981 cagatagtta aacttggata tgcgggaaga gaccagatct cctgtaaaat catcatctgt
     2041 ataacctaac atggtaagga tagcctttac tgcatcttta taaaggaccg gatcctccgg
     2101 tttgaatttt ccacctaagt acccactcat ccagccttct cttgctgcaa tgcggatata
     2161 cactgcattt ggatccgttg ccggcacatc tgcgtaaaca gaaacattgg aagttggcag
     2221 attttcgcgg taactggatg cctttaccag catttttgca aagtccgctc tggtaacact
     2281 ttccctgaaa ctggatatct ccatgatacc ggtcagcttt acaacctgct ggcgcagatt
     2341 aaaattggct gtggaaatat tgtctgccca tgcagttata ctcagtatag gagaaatagc
     2401 cgctgcagcc gccagagagc cgcacaggaa acgtgtgtgg ttttttcgta ctttcataat
     2461 ggaaaactcc tctcaaacaa ttcacatctg tttaaacaac tttttcgtat ttgttcatgt
     2521 ttcgttcatt gtttgaaagt ttatcattgg tttgtgaaca aaatgtgttc tggttgtgtt
     2581 tttttggtca aaaatagtca caggcgggac cttcgttaat agcccaagaa aaacaaatgc
     2641 caccttgcgg tggcgcttgt ttttcttttg ggcttgttac atgagaggtg caatcaatct
     2701 taaaaatctt cctgccttgg tcccgaacca gttgtactgt gcgcagtcct ttaacgtaac
     2761 aggaatacat ttttccagtg tattttcaaa atccttctcc acatctgcaa ctgtactgtt
     2821 tctgtataaa taggttccac attcaaaatg cagatataaa cttctgaaat ccaggttgat
     2881 gcttcccact gctgcctttt catcatcact gacaaagatc tttgcatggg taaaaccagg
     2941 ctcatactcg tagatcttca ctccggaagc gatcagttcc ggatagtacg tccttgccag
     3001 gagataagca tactttttat ccgggatatg gggcataatg atctttacat caatgccacg
     3061 cttggctgca taagccatgg catttttcat cccgtcatcc aggatcagat aaggggtgat
     3121 aatgtgtaca taggactttg cctggtttaa tatatccaga tagacctgct ctcccacctg
     3181 ttcttcatct aagggactgt ctgcataagg catgacaaag ccatctgcct tatatacctc
     3241 cggcagtttg taatccgccg gcaccagata ctgctcataa ttttcttcat tcttactttt
     3301 ggagctggct gcatcccaca tctgcaaaaa catcatggta aaacttttta cagcatctcc
     3361 cttgatcatg atggctgtat ctttccagtg tccaaaacgt tctttcctgt taatatactc
     3421 atctgccagg ttcacacctc ctgtaaaggc ggtatgtcca tcgatcactg taacctttct
     3481 gtgatccctg taattctggt gtgtggaaag cactggtttg attggtgaaa acatctggca
     3541 ccggatgccc atattttcca gtttctgggg ataatgataa ggaaggagca taagacagca
     3601 cataccatca tacatgacac gtacttctac tccctctgcc gctttctgcc ttaaaacctc
     3661 caggatactg tcccacatat agccccggtc aatgataaaa tattccatga agataaaatg
     3721 ctttgctgct tttaattctt ccatcagctg ctcaaacata tcttctccca gagggaagaa
     3781 tttcgctgct gtattttcat acacagggaa acctgcaatg tggttcatat aatctaccag
     3841 acggcttcca cgcacactgg ctgcccgcag ccgcttctgc acctgtgggt cctggacaag
     3901 ataaggtttt gttttttcaa cagacagatt gacccgtctt gccagcgccc ttgtctggaa
     3961 ctggacctgc acaaacacat ataagataat gccgaaaata gggatcagca gtaccggtat
     4021 gatccatgcc attttaaagc tggaatccat aggccggttt aaaatagcga tgacaacaga
     4081 tgagcttacc agtacataag taatataaac aaaatgaata taatttccca accagcggaa
     4141 aatgctgaag aaaaaggcga tctgcaaaag caatcccagg atcacaaaag tggtccttcc
     4201 aaaaatgatc ctgaaaaggc ttctcggtct cggctgtgat ttcatgtata tcgctcctca
     4261 tttcacattt ttcaacaata aaatctactt ctatcataac ttatttaaac aataattatc
     4321 ccactttttt ccataaaatg caatgtacac atgtaagatc attgtcaaat ctatgtcaga
     4381 tcactggatc accctgtttt gatacacaca aaaggcccgg agccatattc tgactccgga
     4441 cctttgtcac gttctttttc ccgtatacat tatatatgta tgttttccta tttcccttat
     4501 ttatccagtt tttccacttc catattcttt ggaaggatca ggttcatgat aattgctact
     4561 acaaacacaa ctgctactac gtttgcagag aatacagact gaacaactgg tgagaattca
     4621 gcccagatac cactttcact ggtagtggta aatccaatac caatggataa tgccattgca
     4681 acaatagtga tatttctctg ggtaaatcca cagttagcga tcatctgcat accagatgtt
     4741 aaaatggtac caaacatcat gatggtacat ccgcccagta cagactgagg aagggatgca
     4801 aagaagttac cgatcggtgg gaacagacct gccaggatca ttgcaactgc accagtcatg
     4861 atagtgaaac ggtttacaac ttttgtcatt gctaccagac ctacgttctg agagaaagag
     4921 gtaactggcg ggcatccgaa cagaccggaa ataaaggaac catagccatc acatgctaag
     4981 gaaccggaaa tctcttctcc tgtgatctca cgtcccaaac cgcctgctac cagtgcagaa
     5041 gtgtcaccaa tggtctcagc agcagatacc aggaagataa tgcatgctga catgatggct
     5101 cctgcgtgga attccggttt gatcggcagc agatgtggaa gtgccacgat gccgccggac
     5161 atgatcatac ttaaatctac tttacccata aagattgcaa caatataacc aacgatcaga
     5221 ccagctaata ctgaaagctg ttttaaatat cccttagcaa acatattcca aagcagacag
     5281 gttgctaatg tgatcagacc taacagcagg tttgaaacgg aaccaaagtc ggttgcatag
     5341 ccaccgccaa aggatctggc acctacagta aacagtgaga aaccaatagc ggttactaca
     5401 gatgctgcta cgatcgggct tatgatcttt ctccagtact ttgcagaaag tcccaaagtt
     5461 ccttcaaaca gaccaccgat caatacggct cctattactg ccggataccc gtaattagct
     5521 gagatcgtac taagtacagt tacgaaggta aagctgacac ccattacaac gggaagtctg
     5581 gatccgatct tccataaagg atataactgg atcatcgttg cgatacctgc tgcgaacatt
     5641 gcgttctgaa gaagcatacc aagctctgct ccggtaaccg gtacctttgc ggcacctgcg
     5701 atcatggtga tcggtgccag gttagatacg aacattgcca aaatatgctg aagaccaaat
     5761 ggaatggcct ttccaatggg cacccgcccg tctagtttgt aaatgttgct gatactgcag
     5821 tttgcattct ctttcatagt ttttgctgcc tccaatcttc ccccgattat tctttatggc
     5881 ttaccttgta aacctcctcc acaatacgtt ccatggcatt taagatattc tcatatcctg
     5941 tgcagcggca cagatgcccg gagatcagtt ttttcagctc ctctctgtta tatttttttc
     6001 ctgttcctac aatttctact gcactcatga tcaggcccgg agtacagaag ccgcactgca
     6061 cagctgcttc ttcaatgaac gccctttgga tcggagacaa ttccccgttt ggtcccttca
     6121 atccttctac ggtcattaca ctctttccgt ctgcccacat ggtgagatat atacagctgt
     6181 caatggcttc tccattgacc agcacagtac aggcaccaca ttctcctact tcacatcctt
     6241 tttttacgga tgtaagtcct aagcggttgc gcagtgtatc cagaagagac tctctctcat
     6301 ccactgcaag ttctgtttcc tttccgttta ctgtcatatg aatgatctta agcatcctct
     6361 tcacctcctg ctcttcttat agcctctgcc aggccacgtt ttgccagctc ttcgatcagc
     6421 tgaagacgga actccttaga agctctccag gaagatcttg gatttacttc tgtgagcgcc
     6481 agcttggcaa actctgctgc tgcctcttta tcagaaacag cctttcctct taaatactct
     6541 tctgctgcgt gacatcttaa aggtgtaggt ccggcaaccc cgtatcccag tcttacatct
     6601 tcaatggtct ttttgtcagc agacagtttt acacgtacag cgcagcctaa ggtcgcgatc
     6661 tccatggctt tacgctttcc atatttaata tactgtccgt accagccttc atagtcctct
     6721 tttttgataa tgaaaccggt gcagacctca catctgtctc ttactgtgcg tcctggtcct
     6781 gtatagaact catggatcgg cacctctctg tgtccttctg gtccttctaa ctggatcagc
     6841 gcgtttaggg tccacatact tgctgcggag tctgcggatg tagcgccgtt gcagatattt
     6901 ccgccaatgg taccggtatt acggatctga ggtccgccta ccatatctac tgcttcgcct
     6961 aacataggaa ccagcttctg gatggtggga tcattggtaa tatgggaaaa tgcagttgca
     7021 gctccgatcc acagatctcc gttttccagg acttttacgc cctttaattc tttaatattg
     7081 tggatggata ccagggctct tcctgcatcc tttccctctc ttacacggat gagtacgtcg
     7141 gtaccgccgc tgatgacttc tgcctcaggc tcccgtacca gtgcttccac tgcatccttt
     7201 acatcttttg cttcataaaa tgatttaata tcaaacatac tcattcacct ccctagatca
     7261 gacctttttc cttgaatttt tcgatcagtc tctgtgctgt cataggactt acgttcatat
     7321 gaacaccggt agcatttaag atcgcatttc ttactgcagg cgcaaccggg attgcaggtg
     7381 gctcacccag agacttgttt ccataagggc ctgttgggtc atctaactgg ataaactcca
     7441 catgaagatc cggcgcatcc atagcagtcg ggatcttata atccagcagg ttgttgttta
     7501 atggacgtcc tgtcttttca tccactaata attcctcgga aagtccaaat ccaaggctca
     7561 tgctcatacc gccatgtacc tgtgcctctg ctgtctttgg attgatcagg ataccggagt
     7621 catgaacatt gatgatatcc tttacagtta ccagtcccag tggcatatct acttctacgt
     7681 ctacaaagca gcagccggag gagaatgtat tatccagaca ctggttggtc acttctgcag
     7741 tcagatgaat ggatttatct aatgaataga aggctgtatc tgctactact gccacatcaa
     7801 agaggaccgg atcaccaccg tgtacaacga tcttgctgtc aacgatgtgt aacatatctt
     7861 ccttaacctc gtcttccgga tggagtccta atgcttcacg aagcgttcca gctgactctt
     7921 ttaccagatc tgcgtacaca gtcttggaaa gatccgtcac atcatgattt aacatgtatg
     7981 ctgcatagtc cagaatacgg tgtcttaatt ccgttgcaca tttcttgcag gccataccgg
     8041 atacataagt ctgtctggaa gcatacgcac ctgtatcaaa tggtgtcagg tctgtatcct
     8101 gggtggatac gataaatacc ttgtcaaagg aaatgcctgt agtctcagag gccatctggc
     8161 tgaatactgt atctgcgccc tgaccgatct ctgtagcacc catggatacc tgcatggagc
     8221 cgtcctggtt taataccata cgtacagaag atgtctcaag tga
//
LOCUS       DBMDUA010000124         8260 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3885_length_8260_cov_2.027577, whole genome shotgun sequence.
ACCESSION   DBMDUA010000124 DBMDUA010000000
VERSION     DBMDUA010000124.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8260)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3885_length_8260_cov_2.027577"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="ACZPCL_09460"
     CDS             complement(<1..127)
                     /locus_tag="ACZPCL_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1162729.1"
                     /translation="MSKAIITVVGKDTVGIIAKVCTYLAENHVNILDISQTIVQGY"
     gene            complement(210..1007)
                     /locus_tag="ACZPCL_09465"
     CDS             complement(210..1007)
                     /locus_tag="ACZPCL_09465"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1162730.1"
                     /translation="MRMVSIASGSSGNCIYIGSDNTHLLVDAGISNKRIQEGLNDIGL
                     TGSDVTGILVTHEHSDHIKGLGVLSRKYHLPIYGTKETLDEIAACKTLGTFDTGLLSP
                     IEPDVDFQIGDLTVKPFKIDHDAVNPVAYRVENGSKAVAVATDMGHFDQYVIDHLQKL
                     DAILLESNHDVRMLETGPYPYYLKRRILGDHGHLSNENAGRLLNNILHDNLKKIFLGH
                     LSKENNYAELAYETVRLEIDEGDCPYGASDFSISVASRDKMSEVVCL"
     gene            complement(1004..1657)
                     /gene="coaE"
                     /locus_tag="ACZPCL_09470"
     CDS             complement(1004..1657)
                     /gene="coaE"
                     /locus_tag="ACZPCL_09470"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604261.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR1162731.1"
                     /translation="MCQDRTDDMLETHFTCKGKKYPVIGLTGGVGAGKSTVLSILQEK
                     YGFHVIQADLVARALMEPGKEAYEAVCAFLGPKILLEDGQINRSAMAEIIFKDPSKRA
                     KVDSLTHPLVWKAVKKEAEEYNRQSVVIEAAIPSKEFRDICDEMWYLYTSREERIRRL
                     KESRGYSDEKIAGIMANQASEEEFRNFTDCEINNSGTEEETSLQIQKLLCGKDNETA"
     gene            complement(1700..4447)
                     /gene="polA"
                     /locus_tag="ACZPCL_09475"
     CDS             complement(1700..4447)
                     /gene="polA"
                     /locus_tag="ACZPCL_09475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR1162732.1"
                     /translation="MDNKLVLIDGHSILNRAFYGVPDLTNAEGLHTNAVYGFLNIMFK
                     ILEEENADHLAVAFDLSAPTFRHKMYDAYKGTRKPMPEELREQVPLMKEALQTMNIPI
                     LTMEGYEADDILGTVAKRCQAEGIEVSVVSGDRDLLQLADTHIKIRIPKTSKGSTQIY
                     DYYPEDVKEQYQVTPTEFIDVKALMGDTSDNIPGVPSIGEKTATNLIVSYGSIENAYA
                     HVDEIKPPRAQKALSEHYDMAQMSKELATICITCPIPFSYEEALIGNLYTPEAFKFMK
                     RMEFKSILNRFDSASMEDKENGPQKSFKEVTDKKEAEKIFKAAEKATVTGLQLITGSR
                     AKVSEEAEQLTFSFDTDGTVHSGKKAKSALLPVTALAICTGPEEIYCIRVSESQITDS
                     WLTEKVQNLCKNDTVHSVWVLDLKSMLPLLNLTDEAPVYDAGVAGYLLNPLKDTYGYD
                     DLARDYLSMTIPSKSDLIGKENLGDLLETGDEKAVTCACYMGYIAWKAAEPLKSRLEQ
                     EEMYKLYTEIEMPLIYSLWHMEQEGVLVKRDKLKEYGDTLKVGIKKLETEIYAETGKE
                     FNINSPKQLGEILFGEMQLPGGKKTKTGYSTAAEVLEKLAPEHPVVQKILDYRQLTKL
                     NSTYAEGLAAYISEDGRIHGKFNQTITATGRISSTEPNLQNIPVRMELGRQIRKVFVP
                     KEGYIFTDADYSQIELRILAHMSGDERLIEAYRESRDIHATTASQVFHVPLEEVTPLQ
                     RRNAKAVNFGIVYGISAFGLSEDLSISRKEAKEYIERYFETYPKVKEFLDSLVKQGKE
                     QGYVTTLYGRRRPIPEFKSSNFMQRQFGERVAMNSPIQGTAADIMKIAMIAVDRELRK
                     RGLSSRIVLQIHDELLIETAENEVEEVKEILTDKMKHAANLKVSLEVDAKTGASWFDA
                     K"
     gene            complement(4477..4821)
                     /locus_tag="ACZPCL_09480"
     CDS             complement(4477..4821)
                     /locus_tag="ACZPCL_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR1162733.1"
                     /translation="MTCREAERLVMPYINGSITDEELKAFVEHIDHCPDCREELEIYF
                     TVDVGIRQLDEGTGSYNIKGALETALELSRQRIHTLKLLQNAIYAVNTLCFWSVFVIL
                     IVQIRIWSQTGF"
     gene            complement(5019..5101)
                     /locus_tag="ACZPCL_09485"
     tRNA            complement(5019..5101)
                     /locus_tag="ACZPCL_09485"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5066..5068),aa:Leu,seq:caa)
     gene            complement(5249..6055)
                     /locus_tag="ACZPCL_09490"
     CDS             complement(5249..6055)
                     /locus_tag="ACZPCL_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR1162734.1"
                     /translation="MKKKNKMAAFLAAVILMTMSVCQGAFAGETAGIDTARPVSLTID
                     LEAESKKATISIYKVGEWNGAEGAYALSQEFTGSGADIGDHTATGVLSAAQTLEQFIK
                     NNQIGALSTQATASGKIQFTGLSSGLYLVCQFKASGDNVTVSPYLTTLPVLNEETNTW
                     VYDVRSLPKYEADSTGGGGHSGGGGGGGGSSTTGRHAAQTNESVDVPDNDVPKAENPI
                     VKAIEDVLVPLGILPKTGDNSTSIEIMSGITVMSAALLVLLIAKRRRKDA"
     gene            complement(6039..7019)
                     /locus_tag="ACZPCL_09495"
     CDS             complement(6039..7019)
                     /locus_tag="ACZPCL_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIR1162735.1"
                     /translation="MRKRISTIIFVIIFLIGLSILLYPAVSEYVNERHSSRAIASYDK
                     LVEELPEADYSKELQDAQAYNEYLAQFGNLSEAASTEKDREDSPYEDLLNTGGSGIMS
                     IINIPGIAVELPVYHGTSEGVLQVAAGHYEGSSLPIGGESTHAIITGHRGLPSAKLFT
                     DLDRLETGDVFYLKTLGEILEYQIDQIVTVLPSEVDGLSIEPGKDYVTLVTCTPYGIN
                     SHRLLIRGTRIPYDGKYAEKVPLKPAPAVSDRPAEEQGRKLGTRQWILFAIAAFGVAI
                     LLGILAPSGKKKGKASSKTEETADQRNKELSQNSKAEKKEGSHEKNEEEK"
     gene            complement(7119..>8260)
                     /locus_tag="ACZPCL_09500"
     CDS             complement(7119..>8260)
                     /locus_tag="ACZPCL_09500"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HIR1162736.1"
                     /translation="VTLNAADFAQYLKSKLTDEEGNPIKTGGLSIKAEEATGAVFTNL
                     DQGYYFVNTTTGSLCSLANAGSYQTIEEKNELPSGEKKIVTDGNVTADSITATIGSTI
                     KYKITVTDKKGTDQAITVHDKMDAGLEFNKDSLEVWRNGVKLTSGYTYDKDATYTDTD
                     NCTFEVVLGAELVKDLQENKTVEIIYTATVGAGAIDAGATSKNTAKIDYSHNTIDIPD
                     IPEVYTYKFGLVKTDKDKNVLSGAKFKLYADSAANTEIKVVKLADGTYRVAVEGETGV
                     EIEAGVTEIKGLGNGTYYLVETEAPQNYNPLTEAVEVKIEDGDKVATLDNNNTVYKEG
                     GVQVINYTGTILPSTGGIGTTIFYAAGIVVMAGAVFFVVRSRKHD"
BASE COUNT         2038 a         1991 c         1615 g         2616 t
ORIGIN      
        1 aataaccctg tacaatggtc tgggagatat ccaggatgtt tacatggttc tctgccagat
       61 aagtacatac ctttgcgata attcccactg tgtcttttcc tactactgtg ataattgcct
      121 tactcatttt tcattctcct cttctttttt tatactcacg aacccgcgtt tttgcgcttt
      181 accgcctgct tacgcaggca gttcgttcgt tacaagcaaa ctacttcact cattttatct
      241 ctggaggcaa cggaaatgga gaaatccgat gctccatacg ggcagtcccc ctcgtcaatt
      301 tccagacgga ctgtctcata agccagttcc gcgtaattat tttccttgct taaatggcct
      361 aagaagattt ttttcagatt atcatgaagg atattattta acaatctccc tgcattttca
      421 ttggacaaat gaccgtgatc cccaaggata cggcgtttta aatagtacgg ataagggccg
      481 gtctccagca tgcgcacatc atggttggac tccaataaaa tggcatccag tttctgaagg
      541 tggtcgatca catactgatc aaaatgtccc atatccgtag ctacagccac agctttggaa
      601 ccattttcca cccggtaagc tacaggattt actgcgtcat gatcgatctt aaagggcttt
      661 acagtcagat cccctatctg aaaatccaca tccggctcta taggggacaa taaaccggta
      721 tcaaaggttc ccaatgtctt gcaggctgcg atctcgtcta aagtctcttt tgtcccgtaa
      781 ataggaagat gatattttct tgaaaggact cccaaccctt taatatggtc ggaatgttca
      841 tgtgtcacca gaatacctgt tacatcagaa ccggtcagtc cgatgtcatt taacccttcc
      901 tggatccgct tgttgctgat acctgcatct actaacagat gggtgttgtc agaacctatg
      961 taaatgcaat tgccgctgct gccgcttgct atgcttacca ttctcatgct gtttcgttat
     1021 ccttcccgca aagaagtttc tgtatctgta aagaagtttc ttcttccgtt ccgctgttgt
     1081 tgatctcaca gtctgtaaag tttctgaatt cttcttccga tgcctgattt gccatgatcc
     1141 ctgcgatctt ttcgtcagaa tatccccggc tttctttcag gcgtctgata cgttcttctc
     1201 ttgatgtata aagataccac atttcgtcgc agatgtcacg aaattctttt gaaggaatag
     1261 cagcttctat gaccaccgac tgtctgttat attcctctgc ctcttttttc actgccttcc
     1321 acacaagggg atgggtcaga ctgtctacct ttgctctttt ggaaggatct ttaaaaatga
     1381 tctctgccat ggcgctgcgg ttgatctgtc cgtcctctaa caggatcttc gggcctaaaa
     1441 aggcgcatac cgcctcataa gcctcttttc ccggttccat aagagctctt gcaacaagat
     1501 ctgcctggat cacatgaaaa ccatattttt cctgaagaat ggacaacacc gtactttttc
     1561 cggctcctac gcccccggtg agaccgatca ccggatattt ttttccttta catgtaaaat
     1621 gtgtctctaa catatcatct gttctgtcct ggcacatagt tctgatctgc tccttaatcg
     1681 tatcctatac gcttatgatc tattttgcgt caaaccagga tgctcctgtt ttcgcgtcaa
     1741 cttccagtga aacctttaaa tttgcagcat gtttcatctt gtctgtaagg atctctttta
     1801 cctcttccac ttcattttct gcagtctcga tcagaagttc atcgtggatc tgtaaaacaa
     1861 tgcgggagga aagccctctc tttcgcagtt ccctgtctac tgcgatcatg gcgatcttca
     1921 taatatcagc tgctgttccc tggatcgggg aattcattgc cacacgttcc ccaaactggc
     1981 gctgcataaa attagaggac ttaaattccg ggatcggacg gcgtctgcca taaagagtcg
     2041 tcacatatcc ctgctctttt ccctgcttta ccaaactgtc cagaaattcc tttactttcg
     2101 gataagtctc aaaataccgt tcaatatatt cctttgcctc ttttctggaa atactcagat
     2161 cctcactgag gccgaaagca ctgatgccgt atacaatacc aaagtttact gcctttgcat
     2221 ttcttctctg aagaggggtt acttcctcta aaggcacatg gaatacctgg gatgctgtag
     2281 tggcatggat atccctggac tccctgtaag cctcgatcag gcgctcatcc ccggacatat
     2341 gggccaaaat acgcagttcg atctgggaat aatcggcatc tgtaaaaata tagccctctt
     2401 tcgggacaaa tacctttcgt atctgacgtc ccagttccat acgcaccgga atgttctgca
     2461 ggtttggctc tgtactgctg atacggcccg tagctgttat agtttggtta aattttccat
     2521 ggatacggcc gtcttcgctg atataagctg caaggccttc tgcataggta gaatttaact
     2581 tggtcagctg gcggtaatcc aggatcttct gtaccaccgg atgctccgga gccagttttt
     2641 ccagaacttc cgctgctgta gaataaccgg tcttggtctt ctttccacct ggaagctgca
     2701 tttcaccaaa caggatctct cccagctgtt tcggggaatt gatattaaat tcttttcctg
     2761 tttcagcata gatctctgtt tccagttttt tgatccctac ttttagggta tctccatatt
     2821 cttttaactt gtcccttttt acaaggacac cttcctgttc catatgccac aagctgtaga
     2881 tcagaggcat ctcgatctct gtatacagct tatacatttc ttcctgctcc aagcggctct
     2941 ttaaaggctc tgcagctttc caggcaatat agcccatata gcaggcacag gttaccgcct
     3001 tttcatcccc tgtttctaac agatcgccca ggttttcttt tccgatcagg tcgcttttag
     3061 aaggaatggt catggaaaga taatctcttg ccagatcatc ataaccatag gtatctttta
     3121 aaggatttaa aagataaccg gcaacgcctg cgtcataaac cggtgcttcg tctgtaagat
     3181 ttaacagagg cagcatggat ttaagatcca ggacccagac agaatggacg gtgtcatttt
     3241 tgcacaggtt ttgtaccttt tctgtcagcc agctgtctgt aatctgagac tcagacacac
     3301 ggatacagta gatttcttcc ggacctgtgc agatcgcaag ggctgttaca ggaagaaggg
     3361 cagattttgc ttttttgccg gaatggactg ttccatccgt atcaaaagaa aatgtcagct
     3421 gttctgcttc ctctgaaacc tttgccctgc ttccggtgat cagctgcaga cctgtaacag
     3481 tggctttttc cgctgcctta aagatctttt ccgcctcttt tttatctgta acttccttaa
     3541 atgacttctg tggaccattt tccttatctt ccatggaagc agagtcgaaa cggtttaaaa
     3601 tggacttaaa ttccatccgt ttcataaact taaaagcttc cggtgtataa agattgccga
     3661 ttagagcctc ttcataggaa aatgggatgg gacaggtgat gcagatcgtt gccagttcct
     3721 tgctcatctg ggccatgtca taatgctcag aaagggcttt ctgggctctt ggaggcttga
     3781 tctcatctac atgggcatag gcattttcaa tagaaccata ggatacgatc agattagtcg
     3841 ctgtcttctc gccaatagaa ggcacacctg ggatattatc agaagtatct cccataagag
     3901 cctttacatc aataaattcc gttggtgtta cctggtattg ttcttttaca tcttccggat
     3961 aataatcata gatctgtgtg ctgcctttgc tggtcttggg gatacggatc ttaatgtggg
     4021 tatccgctaa ctgcaaaaga tcccggtcgc cggaaacaac agatacttcg attccttccg
     4081 cctgacatct ttttgccaca gttcccagga tatcgtccgc ttcatatccc tccattgtaa
     4141 ggatagggat attcatggtc tgcagtgcct ccttcatcag aggtacctgt tcccgcagtt
     4201 cttccggcat cggttttctg gtgcctttgt atgcatcata catcttatgg cggaacgtag
     4261 gcgcagacag gtcaaaggct accgccagat ggtctgcatt ttcttcttcc aatatcttaa
     4321 acatgatatt taaaaagcca tagactgcat tggtatgaag tccttctgca ttggtcagat
     4381 ccggaacccc ataaaaagcc cggtttaaaa tactgtgtcc atcgatcagt accagtttat
     4441 tgtccataat ataaaatcct tttcaataat ttattcttaa aatccagtct ggctccagat
     4501 acggatctgg acgatcagta tcacaaatac agaccagaaa caaagggtat ttactgcata
     4561 aatcgcattt tgcagaagtt tcagcgtgtg gatccgctgg cgggaaagct ccagtgcagt
     4621 ttccaacgca cctttgatgt tgtagcttcc ggtaccttca tccagctgcc tgatacccac
     4681 atctactgta aagtatatct caagttcctc ccggcaatcc gggcaatgat caatatgttc
     4741 tacaaatgct ttcagttcct catctgttat actgccattg atataaggca taaccagacg
     4801 ctctgcctct ctgcaggtca tggcagtcac ctccttttgc agcaaaactg ctgtacgaaa
     4861 taaaacataa ttcagatatc atcatacact aaaatggggc ggttttcaat gtatagaaaa
     4921 atgcttaaat attttttaaa atattttcta aaaagaaaaa aaccttagga acaacgtatt
     4981 cacgctattt ctaaggtttc tccctgccaa gggtgtaatg ccggcggcgg gacttgaacc
     5041 cgcacgcggt tacccgcaac agattttgag tctgcatcgt ctgccattcc gacacgccgg
     5101 catttcttca caacgaaaat tattttacca tatagttttt ggttttgcaa gtggttttgg
     5161 aataagttct aaataagaat gttacagttc agtcatgaca taaaaacagc ggctgcctca
     5221 aattatctgg caaccgctgt ttttattatt atgcatcttt tctcctgcgt tttgcaatga
     5281 gcaataccag gagtgccgca ctcattacgg tgatccctga catgatctcg atggaagtag
     5341 aattatcacc ggtttttggg agaattccta atggaaccag tacatcctca attgctttca
     5401 cgatcggatt ttcagctttt ggaacatcat tgtccggtac gtctacagac tcatttgtct
     5461 gggctgcgtg acgtcctgtg gtactggaac caccgccgcc tccgccgcca ccactgtggc
     5521 caccgcctcc agtggagtct gcttcatact tcggaaggga tctcacatca tatacccagg
     5581 tattcgtttc ttcgtttaat accgggagtg tagtcagata cgggcttaca gttacattat
     5641 caccggaagc cttgaactgg cataccagat acaaaccgga tgaaagtcct gtaaactgga
     5701 tctttccgga agcagttgcc tgtgtagaca acgctccgat ctgattattt tttatgaatt
     5761 gttccagagt ttgtgcagct gacagcacgc cggtagcagt atgatctcct atatcagctc
     5821 cggaaccggt aaattcctga gacagagcat atgctccttc tgcaccattc cattcaccta
     5881 ctttgtaaat ggaaatagtt gcttttttac tttccgcttc cagatcaatg gtcagagata
     5941 ccggtctggc cgtgtcgata cctgctgttt cgcctgcgaa tgcaccctgg catacgctca
     6001 tggtcatcaa tatcactgcc gccagaaaag ctgccatttt atttttcttc ttcattcttt
     6061 tcatggcttc cttctttctt ctctgctttg ctgttctgag agagttcctt gtttctttgg
     6121 tcagcggttt cttccgtttt gctgctggct tttcccttct tctttccgga aggtgccagg
     6181 attccaagta agattgccac tccaaatgca gctatggcaa atagaatcca ttgtcttgta
     6241 cccagtttgc gtccctgctc ttccgccggt ctgtcactga ctgccggtgc cggttttaac
     6301 ggaacctttt ctgcgtattt tccatcatat ggaatacgtg ttccccggat cagaagccgg
     6361 tggctgttga taccataagg agtacaggta accagtgtca cataatcttt tcccggttca
     6421 atggaaagtc cgtctacctc ggaaggaagt acagtaacga tctgatcgat ctgatattcc
     6481 aggatctctc ctaatgtttt cagatagaac acatctcctg tttccaggcg gtccagatcc
     6541 gtaaacagct ttgctgaagg aagtcctctg tgtccggtga tgatcgcatg agtgctttca
     6601 ccgcctattg gaagggagga accttcataa tgtcctgctg ctacctgtaa gacgccttcg
     6661 ctagtaccat gatatacagg aagttctact gcaattcccg gtatattaat gatactcatg
     6721 atccccgaac cacctgtgtt taaaaggtct tcataaggac tgtcttcccg gtctttttct
     6781 gtactggctg cttcactgag attgccaaac tgagcaagat attcgttgta agcctgtgcg
     6841 tcctgcagtt ctttggaata atccgcctcc ggaagttcct ctactaactt gtcgtaagaa
     6901 gctatcgccc gtgaggaatg tctctcattc acatactcac tgaccgcagg ataaaggaga
     6961 atggaaagac cgatcaggaa aatgatcaca aatatgatag ttgaaattcg ttttctcatc
     7021 cggtctttcc gcacctcctg gtttatagtt ccttcataaa acttttttca caatgtcttt
     7081 tataaatcca ttatacgtga tagggattta cgtgtcaatt agtcatgctt tctgctgcgt
     7141 actacgaaga atacagcgcc tgccattact acgatacctg ctgcgtagaa gattgttgta
     7201 ccgatgccac cggtggatgg aaggattgta ccagtatagt tgattacctg aacgccgccc
     7261 tctttatata cagtgttatt attatcaaga gttgctacct tatcgccatc ttcaatttta
     7321 acttctactg cttctgttaa cggattgtag ttctgtggag cctctgtttc cactaaataa
     7381 taggttccat ttccaagtcc cttgatttct gtaacaccag cttcgatttc tacaccagtt
     7441 tcgccttcta ctgcaacacg atatgtgcca tcagctaatt taactacctt aatctcagtg
     7501 tttgctgcac tgtcagcata cagtttgaat ttagcaccag aaagtacatt tttatcttta
     7561 tcagtcttta caagaccaaa tttataagta tatacctctg ggatatcagg aatatcaata
     7621 gtattatgtg aataatcaat ctttgcagtg ttcttgctag ttgcgcctgc atcaatagca
     7681 cctgcaccta ctgtagctgt ataaatgatc tctacagtct tattttcctg taagtcttta
     7741 actaattctg cacctaatac aacttcaaaa gtacagttgt ctgtatctgt atatgtggca
     7801 tctttatcat atgtatatcc gctggtaagt tttacaccat ttctccaaac ttcaagggaa
     7861 tccttattaa attccagacc agcatccatt ttatcatgaa cagtgattgc ctggtcggtg
     7921 cctttcttat cagtaactgt gattttgtac ttaatagtag atccgatagt agccgttatt
     7981 gaatccgcag ttacgttacc atcagttacg attttctttt caccagatgg taattcgttc
     8041 ttttcttcaa tagtttgata tgagccagca tttgctaaag agcataaaga acctgtagta
     8101 gtatttacga aataatatcc ctgatccaag ttagtgaata cagcacctgt agcttcttca
     8161 gctttaatac tcaaaccacc agttttaata gggttgcctt cctcatcagt gagttttgat
     8221 tttaaatact gagcaaaatc tgcagcattt aatgtaacaa
//
LOCUS       DBMDUA010000125         8169 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3936_length_8169_cov_2.319286, whole genome shotgun sequence.
ACCESSION   DBMDUA010000125 DBMDUA010000000
VERSION     DBMDUA010000125.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8169)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8169
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3936_length_8169_cov_2.319286"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(24..224)
                     /locus_tag="ACZPCL_09505"
     CDS             complement(24..224)
                     /locus_tag="ACZPCL_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR1162737.1"
                     /translation="MDYPNKDQRWVFHEKTEAQETAPGVVRRVLAYCDNVMCVENTFK
                     KGSIGAMHNHPHTQITCVGTCT"
     gene            385..1104
                     /locus_tag="ACZPCL_09510"
     CDS             385..1104
                     /locus_tag="ACZPCL_09510"
                     /inference="COORDINATES: protein motif:HMM:NF019322.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1162738.1"
                     /translation="MTNKPIYIIIYDKLKSAIKGGTYPPGSFLPTEQELETLYQVSRT
                     TIRRAVKLLSDEGILSVRQGCGTMVMDIRTTQNYNQVTSVTESLRKRGYDVTTGSMMI
                     DVIPASRDIAADLFIPEGTPVARIQRLQLADGRPVTLMENYIEYAKVPGIEQHSGHFV
                     ALYQFLEETYGLIIDATRDRISAKSADFLEARVLEIEPKTALLVIRRISYYQDKPVSI
                     DHVRIIGSQYEAEISGKGRSK"
     gene            1106..1774
                     /gene="deoC"
                     /locus_tag="ACZPCL_09515"
     CDS             1106..1774
                     /gene="deoC"
                     /locus_tag="ACZPCL_09515"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR1162739.1"
                     /translation="MCLTELAKKIDISCVQAFHTRDEIDSMIEAAIRYHFAAAFTLPA
                     FSSYIAKKLADYPDIHTGGVISFPGGGDTIFQKSRQAKELREAGCKELDMVMNLTALR
                     SHEDNYVLEDIGAVREAAGKDILKVIIEAPQLTEEEIRRAVNLCIRAGADYAKTSTGW
                     YPAHPSALSQVQIMTDEAKGRIKIKAAGGIRTLETIHAMEKAGCDRFGIGLSSALKLF
                     TDLS"
     gene            1984..3045
                     /locus_tag="ACZPCL_09520"
     CDS             1984..3045
                     /locus_tag="ACZPCL_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR1162740.1"
                     /translation="MKGYAMLKIGESGWIEKDRPACGPMDAICKPLAVAICTSDVHTL
                     WEGAIGDRHNMILGHECCAEVVEVGSMVKDFKPGDRVLVPAITPDWNSLEAQAGFSMH
                     SGGMLAGWKFSNFKDGVFSEYFHVNDADGNLALLPSNISPTDACMLSDMVPTGFHGVE
                     LADVQFGDTVLVVGIGPVGLMSVAGTNMRGASRIIAVGTRPVCVEAAKKYGATDFVSY
                     KNGPIWKQVMDMTDGKGVDKVVIAGGGVETFAEAVKCLKPGGKIGNVNYLGSGDTIDI
                     PRTEWGVGMGHKQINGGLMPGGRLRMEKLGALVSAGKLDVSPLSTHVFKGWDHLEEAL
                     FLMRDKPADLIKPVVVIEE"
     gene            3160..4098
                     /locus_tag="ACZPCL_09525"
     CDS             3160..4098
                     /locus_tag="ACZPCL_09525"
                     /inference="COORDINATES: protein motif:HMM:NF014542.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR1162741.1"
                     /translation="MKILIVSGFLGAGKTTFIKALAKHTKKDFAILENEYGAAGIDAD
                     RLRAEEGQSPVNIWEMTEGCICCSAKGDFSLSVLSIANTIDPEYLVIEPTGVGMLGNV
                     MENLKQIEYEHISILAPVTIADIYSYRRYLKEYPALYENQIKDAGTIFLSKSEQASAE
                     EKEEARQVLAGINPTATIYTDHYSSLPEKDWLRLLETGYDGRLYQVKAEETENLPDTF
                     SIENAYFSSPESLLIFLEDLIRGQYGNIFRAKGQILAGSQWFQFDVADSRYGITGCEP
                     QKTGKAVFIGTEIHRQPIRRVVLSKMIQKKIVVKKR"
     gene            4280..5647
                     /locus_tag="ACZPCL_09530"
     CDS             4280..5647
                     /locus_tag="ACZPCL_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1162742.1"
                     /translation="MSKNKRSTFSGKLGFVLSAAGASVGLGNIWRFPYLAAKYGGGIF
                     LLVYIILALTFGYTMIMAETSLGRMTKKSPVGAYQSFGKSKWLSLGGWINAIIPILIV
                     PYYSVIGGWVIKYLIGYFSGQAQTLASDTYFSAFISNGASTEICFIVFTFCTLGIIYA
                     GVQNGIERVSKFMMPVLVVLSVIIAIYSVTRPGALEGVKYFLVPNPANFSWMTVVSAM
                     GQMFYSLSIAMGILVTFGSYMLKDMSIEDSTQNVEIFDTAIAIMAGLMIIPAVFAFSG
                     GDPDTLNAGPALMFITIPKVFANMGLGTGIGILFFLLVLFAAMTSSIALTESAVSTFE
                     DELYWGRKKSTIVMAVIMVSLGSLSCLGYGPLANVTIIGMQFLDFFDFLTNSVMMPIA
                     AIATCLLVSRVIGVKKIEEEVTLEGKPFRRKKIFYFMIQYLCPIFAAIILISSVANAF
                     GWISM"
     gene            complement(5748..6932)
                     /locus_tag="ACZPCL_09535"
     CDS             complement(5748..6932)
                     /locus_tag="ACZPCL_09535"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1162743.1"
                     /translation="MINFEFSNTTEIVFGKGTQEQVGEKIRKYSDCNKVLVVYGSDRI
                     KKNGLFDAVSSSLKQQKIDILELGGVKPNPRLSLVRRGIEICRNEKIDFILAIGGGSA
                     IDTAKAIGVGVPYEGDVWDFYLGKDTPKKTLKTAVICTIPAAGSESSLSSVITNEDGW
                     YKKSINLDLIRPVFAIINPELTFTLPAYQIGCGVVDMMTHIMERYFTNTTHVDLTDRL
                     CEAALRSIIANGEKTLKEPENYDTRAEIVWAGTLAHNGLLNTGRVQDWASHRIALEMS
                     GLYDIAHGASLSIVYPAWMEYVYSHDKKRFMQFAVRVWDVDLAYGEEDEICLEGIRRM
                     REFFKKLGMPLTFEDAGLPTDAFEEMADKATYGDTIPVGNFVKIGKSQMIDIYKLCTE
                     RK"
     gene            complement(6947..7810)
                     /locus_tag="ACZPCL_09540"
     CDS             complement(6947..7810)
                     /locus_tag="ACZPCL_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HIR1162744.1"
                     /translation="MLINMEEMLKVADKNQFAVGAFNATESSLFRAVVEEAEAQNAPA
                     IVQVAPGEFDFATRDFYQYVRARLSNSKVPFVLHLDHGKTIQQCMKAIQAGFTSVMLD
                     GSELPYEENVRLTKEVTDLAHMVGVSVEGEIGTIGVMSNSDEGGVENVTYTNPEDVID
                     FVTKTGVDCLAIAIGTAHGIYPKGFVPKLQLELLERIKEVAPVPLVLHGGSNNPDDEI
                     RRACQIGIRKVNISSDFKYAFFKKVDEVIQETGGFVPAKVLTPGINDAKQVIREKMEL
                     FGSLGKAELYR"
     gene            complement(7857..8087)
                     /locus_tag="ACZPCL_09545"
     CDS             complement(7857..8087)
                     /locus_tag="ACZPCL_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162745.1"
                     /translation="MMWGCGSPVPHGGMLSVPLFTNPAKFCLALAIGSVITGVILSLL
                     KKPVTEEDESFEDLGTETGAASDDGEIKIETF"
BASE COUNT         2201 a         1902 c         1658 g         2408 t
ORIGIN      
        1 tattctccga ttatggtgac ttcttacgta caggtgccaa cacaggtgat ctgggtatgt
       61 ggatggttgt gcattgctcc gatggaacct ttcttaaagg tattttccac gcacataaca
      121 ttatcacaat aagctaagac ccggcgcaca actcctggtg ctgtctcctg tgcctctgtt
      181 ttttcatgaa aaacccatct ttgatcttta tttggatagt ccataattcc tccttgttga
      241 tttgattgac atatataata agcttattat acgttcctta tggggatgcg tcaagagtta
      301 acgtttactt ttttaaatat aattcattaa atttgcgttt tttctgaaaa atatatataa
      361 tggagcccag ggggaatact taaaatgaca aacaaaccga tctacataat aatatacgat
      421 aaattaaaat ccgctatcaa gggcggcacc tacccgccgg gaagttttct tcccacagag
      481 caggaattgg aaactttata ccaggtcagc cgcactacca tccgcagggc tgtaaaactg
      541 ctttctgatg aaggtatttt aagtgtgcgc cagggctgtg gtacgatggt catggacatc
      601 cgcactactc agaattataa ccaggtcact tctgtaactg agtctttgcg aaaacgtggc
      661 tatgatgtga ccacaggaag catgatgatc gatgtgatcc cggcttccag ggatattgct
      721 gcagaccttt ttataccgga aggaacgccg gttgcccgga tccagagact gcagctggca
      781 gatggcagac cggtaacttt aatggaaaac tatattgaat atgcaaaagt ccctggcatt
      841 gagcagcaca gcggccattt cgttgccctg tatcagtttt tagaagagac ctatggcctg
      901 atcatcgatg ctaccaggga ccgtatttca gccaaatctg cagatttctt agaagcccgt
      961 gtcctggaaa tagaacccaa aaccgctctt ttagtgatcc ggcgtatttc ctattatcag
     1021 gataagccgg tgagtattga ccacgtgcgc atcattggca gccagtacga ggcagagatc
     1081 tcaggaaaag gaaggagcaa ataatatgtg tctcacagaa cttgcaaaaa agatcgacat
     1141 cagctgcgtc caggcattcc atacccgtga tgagatcgac tctatgatag aagctgccat
     1201 ccggtatcat ttcgctgctg ctttcaccct tcctgccttt tcttcttata tagcaaaaaa
     1261 gctggctgat tacccggaca tccacacagg aggcgtcatc agctttccgg gaggcggtga
     1321 tactattttc cagaaaagca gacaggcaaa agaactgcgt gaagccggtt gtaaagagct
     1381 tgatatggtt atgaatttaa cagctttgcg gagtcatgaa gacaattatg tactggaaga
     1441 tattggtgct gttcgggaag cagccggtaa agatatctta aaggtcatta ttgaagcacc
     1501 tcagctgaca gaggaagaaa tacgcagggc agtaaattta tgcatccggg ccggtgctga
     1561 ttacgcaaaa acaagcacag gctggtatcc tgcacaccct tctgccctct ctcaggtaca
     1621 gatcatgaca gatgaggcaa agggacgtat taagatcaaa gctgccggag ggatccgcac
     1681 attagaaacc atccatgcca tggaaaaagc cggctgtgac cgttttggca tcggtctctc
     1741 ttctgcttta aagcttttta cggacctgtc ttaatgatcg atcctgtatc tgccctttta
     1801 aagatacctt tcaataacct ttctggcatt tattctaaaa agatacctga aacattgtga
     1861 aatatgccta taaagtcacc attgatttta tgacctttta gtgatatatt tttaactatc
     1921 agatgatatt tttttaacaa acaaatacta tataaatgca tcattgaaag gagaacatta
     1981 tttatgaagg gttacgcaat gttaaaaatc ggagaatctg gctggattga gaaagaccgt
     2041 cctgcatgtg gaccaatgga cgctatctgt aagccattag cagttgctat ctgtacttcc
     2101 gatgtacata ctctctggga aggcgctatc ggcgaccgcc acaacatgat cttaggacat
     2161 gagtgctgtg cagaagtagt tgaagttggt tctatggtaa aagatttcaa gccaggcgac
     2221 cgtgtattag ttcctgctat cactcctgac tggaactcct tagaggctca ggctggtttc
     2281 tccatgcact ccggcggtat gttagcgggc tggaaattct ccaacttcaa agatggcgta
     2341 ttttccgaat acttccatgt aaatgatgca gacggcaacc tggcactgct gccatccaac
     2401 atcagcccaa ctgacgcatg tatgctgtct gatatggttc ctaccggttt ccatggcgta
     2461 gagctggcag atgttcagtt tggtgatact gttttagtag ttggtatcgg acctgtagga
     2521 cttatgtctg tagcaggaac caatatgcgc ggcgcttccc gcatcatcgc agttggaacc
     2581 cgccctgtct gcgtagaagc agctaagaag tacggcgcta ccgatttcgt aagctacaag
     2641 aacggcccga tctggaaaca ggttatggat atgacagacg gcaagggcgt tgacaaagtt
     2701 gtcatcgcag gcggcggcgt tgaaaccttc gcagaagcag ttaagtgctt aaagccaggc
     2761 ggcaagatcg gtaacgtaaa ctaccttggc tccggcgata ccattgatat cccacgtaca
     2821 gaatggggcg ttggtatggg tcacaagcag attaacggcg gtttaatgcc aggcggacgt
     2881 ttacgtatgg aaaaactggg cgctttagta tctgccggaa aactggatgt ttccccatta
     2941 agcactcatg tattcaaggg ctgggatcac ctggaagaag cattattcct tatgagagac
     3001 aagccagctg acctgatcaa gccagttgtt gtgatcgaag aataaagact ttaatggcta
     3061 aatagcatac ttatcttgag gacgcgcttc tggcgcgttc tcgttctact acaggaaaac
     3121 agagcacgta ctctgggaat tggggatttt ggttatcata tgaaaatact gatcgtatcc
     3181 ggcttcctgg gcgcaggaaa aactactttt atcaaggcat tggcaaaaca cacaaaaaaa
     3241 gactttgcca tactggaaaa tgaatacggt gccgctggca ttgacgcaga ccggctgcgt
     3301 gcagaagaag gtcagagccc tgtcaacatc tgggaaatga cagaaggctg catctgctgc
     3361 tctgccaaag gagatttttc cctgtccgtt ctctccattg ccaataccat tgacccggaa
     3421 tatctggtca tcgaacctac cggtgtcggt atgcttggca atgtcatgga aaatttaaag
     3481 cagatcgaat acgagcatat ttccattttg gcacctgtga ccattgcaga tatctacagc
     3541 taccggcgct atttaaaaga atatcctgct ctttacgaga accagatcaa agatgccgga
     3601 actattttcc tgtcaaagtc cgaacaggct tctgctgaag aaaaagaaga agccagacag
     3661 gtcttagccg ggatcaatcc cacagctacg atctatacag accactactc ttctcttcct
     3721 gagaaagact ggctgcggct tcttgaaaca gggtatgacg gacggttgta ccaggtaaag
     3781 gcagaagaaa ccgaaaacct tccggatacc ttttccattg aaaacgccta tttttcttcc
     3841 ccagagtctc ttcttatatt cttagaagat ctgatccggg gacagtacgg aaatattttc
     3901 cgcgccaaag gacagatcct tgccggttcc cagtggtttc agtttgatgt agccgacagc
     3961 cgttatggca ttacaggctg tgaaccccaa aagactggaa aagctgtatt catcggcaca
     4021 gagatccaca gacaacccat ccgccgtgtg gtattaagta agatgatcca gaaaaaaatc
     4081 gtcgtaaaaa aacgatgaac attgacagtc tgagcatacc tgatcttccc aaaaacgcct
     4141 ttcaccaggc gttttttaat gttctctttt taaaaagtgt ctggaaacat atgcctgtaa
     4201 ttgcatttta tcttctccta tgttaaactg ttttagatat agcaaaatca tatttataaa
     4261 aataaagggg ttaaatcaca tgagcaaaaa caaacgaagt acattctccg gaaaactggg
     4321 ctttgtactg tctgctgccg gtgcttctgt aggacttgga aacatctggc gatttccgta
     4381 tctggctgcc aaatatggcg gcggcatctt tttactggtc tacatcatcc tggctcttac
     4441 cttcggctac accatgatca tggctgagac atctttggga cgaatgacca aaaagagccc
     4501 ggtaggcgct taccagagtt ttggaaaatc caaatggctc tctcttggcg gctggatcaa
     4561 tgccattatc ccgatcctga tcgttcctta ctactccgta atcggcggat gggtcatcaa
     4621 atatctgatc ggctatttca gcggacaggc gcagacactt gcctctgaca cttatttttc
     4681 cgcttttatc tccaatggag cttccacaga gatctgcttt attgttttta ccttctgcac
     4741 cttaggcatc atctacgcag gtgtgcagaa tggtattgaa cgtgtttcca agtttatgat
     4801 gccggtcctg gtagttcttt ccgtgatcat tgccatttat tctgttacca gacctggtgc
     4861 tttggaaggc gttaaatact ttctggtacc caatccggca aacttttcct ggatgacagt
     4921 tgtttccgcc atgggccaga tgttctactc tctgtccatt gccatgggta tcctggtgac
     4981 tttcggctcc tacatgttaa aggatatgtc cattgaagac tccacccaga atgtagagat
     5041 tttcgatact gctatcgcca ttatggcagg tcttatgatc atccctgcag tatttgcttt
     5101 ctccggcggt gatcctgata cactgaacgc cggtcctgca ctgatgttta ttaccattcc
     5161 aaaagtattt gccaatatgg gccttggaac cggtatcggt atcctgttct tcttactggt
     5221 attatttgca gcaatgacca gctccatcgc actgaccgaa agcgccgttt ctacctttga
     5281 ggatgagttg tactggggac gtaaaaaatc taccattgtt atggcagtga tcatggtatc
     5341 ccttggttct ttatcctgtc tgggatacgg acctttggca aatgtgacca tcatcggcat
     5401 gcagtttctg gatttctttg atttcctcac caactctgtg atgatgccga tcgcagccat
     5461 tgccacctgc cttctggtct ccagagtcat cggtgtaaaa aagatcgaag aagaagtcac
     5521 cttagaagga aagccattcc gaagaaaaaa gatcttctat tttatgatcc agtacctgtg
     5581 ccctatcttt gcagccatta tcctgatcag ttccgtggct aatgcatttg gatggatttc
     5641 tatgtagttt ttaccaataa ctcttatatt gtttcctatg ccaattaaaa gactgctgca
     5701 aaatggattt tctccgttat gcagcagtct ttatactatt taccttatta ttttctttct
     5761 gtacacagtt tataaatatc aatcatctga gactttccga tctttacaaa attacctact
     5821 ggaatcgtat ccccataagt tgccttatcg gccatttctt caaacgcatc tgtcggtaat
     5881 ccggcatctt caaacgtcaa aggcattccc agttttttaa agaattctct catacgtctg
     5941 ataccttcca gacatatttc atcttcttct ccatatgcca gatctacatc ccatactctt
     6001 actgcaaact gcataaagcg ttttttatca tgactgtaaa catattccat ccatgcagga
     6061 tatacaatag aaagacttgc tccatgagca atatcgtaaa gtccgctcat ttccagcgcg
     6121 atacgatggg acgcccaatc ctgtactctt cctgtattta ataatccatt atgagcgaga
     6181 gttcctgccc agacaatttc ggctcttgtg tcataatttt ccggctcttt taaagttttc
     6241 tctccattgg caataatact gcgaagtgct gcttcacata accgatctgt cagatccaca
     6301 tgtgttgtgt tggtaaaata gcgttccata atatgggtca tcatatctac aactccacag
     6361 ccgatctggt atgcaggcaa tgtaaatgta agttcaggat ttataatggc aaataccggg
     6421 cggatcaggt ccagattaat actcttttta taccatccat cttcatttgt aataacagag
     6481 cttaaacttg attcacttcc agctgccggt atagtacaaa ttacggctgt ttttagggtt
     6541 ttctttgggg tatctttacc aagataaaaa tcccagacat ctccctcata agggacacca
     6601 acaccgattg ctttcgcagt gtcaatagca cttccgccac cgatcgcaag gataaaatca
     6661 attttctcat tgcggcatat ttcaattcct ctgcgcacca gacttagcct cggatttggt
     6721 ttcacgcctc ccaattccag aatatcaatt ttctgctgtt tcaatgaact gcttactgca
     6781 tcaaaaagcc catttttctt aatacggtct gagccatata ctacgagtac cttattacag
     6841 tcactgtatt ttcgtatttt ctctccaacc tgttcctgcg taccttttcc aaacacaatt
     6901 tctgtcgtat tagaaaattc aaaattgatc atatttcttc ccgtccttat ctgtataatt
     6961 ctgcctttcc caaagatcca aataactcca ttttctcccg gatcacctgt tttgcatcat
     7021 tgattcctgg agtcaatact ttagcaggaa caaatccacc tgtttcctga atcacttcat
     7081 ctaccttctt aaagaacgca tatttaaaat cacttgaaat attcactttt cggattccaa
     7141 tctggcatgc cctacggatc tcatcatccg gattgttgct tccaccatga aggactaatg
     7201 gtacaggtgc aacttctttg atccgctcta acagctccag ctgaagtttt ggcacaaatc
     7261 cttttggata gattccatgc gctgttccaa ttgcaattgc aaggcagtca acccctgttt
     7321 tggttacaaa atcaatgaca tcttccggat ttgtatatgt aacattttct actccacctt
     7381 catctgagtt actcataact ccgatggttc caatctcacc ttccactgat actcctacca
     7441 tatgtgccaa gtctgttact tcttttgtga ggcggacatt ttcctcataa ggcagttctg
     7501 aaccatcaag cataactgat gtgaaacctg cctggatcgc tttcatacac tgttgaatgg
     7561 tttttccatg atccagatga agaacaaagg gaactttact gttagataat cttgccctta
     7621 catactgata aaaatctctt gtggcaaaat caaattcacc tggtgctacc tgtacaatgg
     7681 ccggcgcatt ctgcgcttct gcctcttcta ccactgcacg aaacagtgaa ctctccgttg
     7741 cattaaatgc acctactgca aactgatttt tgtctgctac ttttaacatt tcctccatat
     7801 tgatcagcat atttattttc ctccttaaat attatttatt ctcccattca tttcccttaa
     7861 aatgtttcta ttttgatctc cccatcatct gaagctgcac cagtctctgt tcctaaatct
     7921 tcaaagcttt catcttcctc ggttacaggt tttttaagca atgataagat gactcctgtt
     7981 atcacactgc cgatagcaag tgccagacag aactttgccg gattagtaaa caacggtaca
     8041 gaaagcattc cgccatgagg tactggtgag ccgcatcccc acatcataga taaaccacct
     8101 gcaactgcgg aaccacatac acaggagaaa acctaacaag acatctactt tcatgtttct
     8161 tcgttttat
//
LOCUS       DBMDUA010000126         8154 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3945_length_8154_cov_2.463926, whole genome shotgun sequence.
ACCESSION   DBMDUA010000126 DBMDUA010000000
VERSION     DBMDUA010000126.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8154)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3945_length_8154_cov_2.463926"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            133..1341
                     /locus_tag="ACZPCL_09550"
     CDS             133..1341
                     /locus_tag="ACZPCL_09550"
                     /inference="COORDINATES: protein motif:HMM:NF014500.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HIR1162746.1"
                     /translation="MSGFANYAKNYFPIYLFGAVFAKLMDISGNAQSIAQFCTLKLGK
                     SRAVLSVVLTCAILTYGGVSLFVVAFVMLPVAIAVFRAADIPKRLIPGAIALGAFTFT
                     MTALPGSPQVQNTIPMTYFGTDSWAAPVLGIIATVIMFSGGMAWLSYRTNKAKKAGEG
                     YGNYQDEIVSDNKEVPGIGLAFAPILIILVVNLVLSKMVYPNVDGSYLEESFNSTLAA
                     NSGTWSVLLGMLVAILFIAAVNFSKLKDGLKDNLKKAANESLAPLINSCAVVGFGSVT
                     KSLAIFGIIQAFALGISSNALLSEVLSINILCGMTASASGGLGAALEALADTYLASGI
                     APQVLHRVAAVAAGGLDSLPYNGATITVLALVGMTHKESYLDMFVVSVVIPLIAAFAL
                     AALASMGICF"
     gene            1368..2102
                     /locus_tag="ACZPCL_09555"
     CDS             1368..2102
                     /locus_tag="ACZPCL_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR1162747.1"
                     /translation="MTDYVSDAQARSLILDVGRRLYHSGFVASNDGNITCKVGDNAIW
                     ATPTGVSKGFMTEEMLVKMDLDGNILEGTYKPSSEIKMHLRVFKENPQVQGVVHAHPL
                     TATAFACAGMSLDELVLVEGILALGCVPLAHFALPGTTDVPDSIAPYCKNYNAVLLAN
                     HGALTWGTSLIQAYYRMETTEYYAKILMMNNFILGKKALLSQQQIDDIIAIRTRMGVT
                     SGGRPTGPEMASNLEDVCLSAPGKEK"
     gene            complement(2828..3769)
                     /locus_tag="ACZPCL_09560"
     CDS             complement(2828..3769)
                     /locus_tag="ACZPCL_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1162748.1"
                     /translation="MNLPDYTNCTLCPRMCHVNRSVTIGYCGCSSHISAARAALHKWE
                     EPVLSGPEEEPGGSGAVFFSGCTLRCCFCQNSLLSKDHFGKKLTVNELGDAFLRLQDK
                     GAYNINLVTPTQFFPDIIKALDLVRHKLHIPVVCNCGGYERPEIISQLKDYINIWLPD
                     FKYFDNELAFRYSKAKDYFEQASASIAQMIHQTGAPEYTLHPSSVPGRPDFPLMTKGV
                     IIRHMVLPGHKDDSIRLLYWIHQHFPEHSYLISLLSQYTPYTKNPDYPQLNRRITSYE
                     YNKVVDTALELGMTDGFMQQKSSAKEEYTPPFNLEGL"
     gene            complement(3792..4655)
                     /locus_tag="ACZPCL_09565"
     CDS             complement(3792..4655)
                     /locus_tag="ACZPCL_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1162749.1"
                     /translation="MDFNKRQYMRPVDFELFYYNDTELRSVASHHHDYCEFYFFLEGN
                     VKYHIGNAIYKLSYGDCLLIPPGIPHFPEFLSFDQPYRRFVLWLNRSYYEKLHKQDAD
                     LTYCFDTAASNQIYRIPTDRITAQDIQGKLLDLLEENSSERIFHAQASELMAMSFLVY
                     INRLLYSSLHEHSQVFENALYLKICDYIHQNLEDDLSLERIAANFYVSKYHIAHIFKD
                     NMGISLHQYILKKRLHASRQAILSGEPISRIFPQYGFKDYTSFFRAFKKEYGISPKEY
                     REQHRLIKTEP"
     gene            complement(4767..5318)
                     /locus_tag="ACZPCL_09570"
     CDS             complement(4767..5318)
                     /locus_tag="ACZPCL_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1162750.1"
                     /translation="MEKINEIVANNIAILRKKNRLSLEELGKLSGVSKSMLALIEKGD
                     GNPTLGILWKIANGLGVPFDELTRRPDPMNHVVKISEIEPIPIADGTARNYSVFSDDK
                     NRRFSVFYLEMDKDCHWNSEPHLQGTIEFFTVTEGSVKIINDGEEFILNKGDGIQFRG
                     DVPHSYHNIADCQSTAYNIMYNP"
     gene            5526..6710
                     /locus_tag="ACZPCL_09575"
     CDS             5526..6710
                     /locus_tag="ACZPCL_09575"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR1162751.1"
                     /translation="MKYDFDSCIDRTKVMSIKWSSKLRQEMYGESDVIPLGIADMDFK
                     AAPAVAKAIQDRAAHENYGYGYLADEFLMACVNWQKRRNHWDIKKEWITYTPGLNLPL
                     VFAIELFTDPGDNVIIQSPVYYPYYDYVNKTGRHIVYNALVNKDGYYEMNFEQLEMQA
                     KDPKTKLILVCSPQNPSGRLWTKEELTRMGNICIDNGVKVVVDEIHSDLILTDREFVS
                     FATISDKFAQNSIICTSPSKSFNVAGLLVSDIIIPNDEMRAAFQEKMQPYYLWPGAFG
                     AVAQIAAYNESEEWLEELKEYLRGNAQFIADFIQKRMPKVKYRIPEATYLGWLDFRAY
                     GMTDQELWDFMCHKAKVATDDGPKFGPNGEAGGFQRINFSCPRAQLEEALNRIAEALD
                     AL"
     gene            6732..7913
                     /locus_tag="ACZPCL_09580"
     CDS             6732..7913
                     /locus_tag="ACZPCL_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1162752.1"
                     /translation="MNDPDILKLVKEQEDYLIKMRRYFHMNPELSNKEFETRKTIISE
                     LEKMGVEYRLAAGTGLVAIIKGGKPGKHHLLRCDMDALPVKEDTDNLIQPKACVSQKP
                     GVCHACGHDAHMAMMLTTIKVLSQVREQLKGTVYCCFEEGEEALDGYQHVLELVSEYP
                     IDDCFALHVYNDMPAGKINLVSGPRMAGDVCFGVHVIGKSGHGSRPDLAKNPVIPASH
                     IVCELDSALQNQMNAEETLTFSICVQKAGEIWNAIPDEAFFSGTSRYFSRPAGEKVLA
                     LIKKTCTNVADVHGCRVEFEPYTKVLMEPVINDQEVVEETAQKLTQMCGPQVLGEHGL
                     WFASETFCKYLNKYPGALGFLGIANPDLGSGAAHHNSRFDVDESALLLGVCAELSFAL
                     R"
BASE COUNT         2437 a         1502 c         1896 g         2319 t
ORIGIN      
        1 aactgttata tataaaacaa gaacgacaag cctgattcac aagcttatcg ctcttaatta
       61 tcatagaaga atcttattta cagagatttt ctcatactct cacattttat ggcgtcttac
      121 accattacat atatgtccgg tttcgcaaat tacgcgaaaa actattttcc aatctatctg
      181 ttcggagcag tttttgcaaa gttaatggat atttcaggaa acgcacagtc tatcgcacag
      241 ttctgtactt taaaattagg aaaaagccgt gccgtacttt ctgttgtatt gacttgcgct
      301 attttaacat acggcggtgt ttccttattc gtagtagcat ttgttatgct tcctgtagca
      361 atcgctgtat tccgcgcagc tgatattccg aagcgtctca ttcctggtgc tatcgctctt
      421 ggcgcattta cctttaccat gacagcactt cctggaagcc cacaggttca gaataccatt
      481 ccaatgactt attttggaac agactcctgg gcagcacctg tccttggtat cattgcaact
      541 gttattatgt ttagcggtgg catggcatgg ctgtcttacc gtactaacaa agcgaaaaaa
      601 gcaggggaag gatatggaaa ttatcaggat gagatcgtat ccgataataa agaagttcca
      661 ggtatcggac ttgcatttgc tcccatcctg atcattctgg tagtaaacct ggttctttct
      721 aaaatggtat atcccaatgt tgatggaagt tatctggaag aaagctttaa cagtacactg
      781 gctgcaaaca gtggtacatg gagcgtactt cttggtatgc tggttgccat ccttttcatt
      841 gcagctgtta actttagcaa attaaaagac ggattaaaag acaatctgaa aaaagcagca
      901 aacgagtctc tggctccgct gatcaacagc tgtgcggttg ttggctttgg ttctgtaaca
      961 aaatccctgg ctatttttgg cattatccag gcttttgcac tgggaatttc ctctaatgca
     1021 ctgttaagtg aagttctttc tattaatatt ctctgcggaa tgaccgcttc tgcatcgggt
     1081 ggactgggag cagcactgga agcactggca gatacctatc tggcgtctgg cattgcacct
     1141 caggttcttc atagagtggc agctgtagct gcaggtggtc tggacagtct tccatataac
     1201 ggagcaacca ttactgttct ggcactggta ggcatgacac ataaggaatc ttacttggat
     1261 atgttcgtag tatccgtagt gatcccgttg atcgcagctt ttgctctggc agcactggcg
     1321 agtatgggaa tctgcttcta aatttaattt taagaaggag aagaaaaatg actgattatg
     1381 tttcagatgc acaggcccgt agcctgatcc tggatgtggg gcgtagactg taccacagcg
     1441 gctttgtagc atctaatgac ggaaatatta catgtaaggt tggagataat gcgatctggg
     1501 ctactcctac aggcgtatcc aaaggtttta tgacagagga aatgcttgtg aaaatggatt
     1561 tggacggaaa tatactggaa ggcacttaca agccttccag cgagatcaaa atgcaccttc
     1621 gtgtgtttaa agaaaatcca caggtacagg gcgtggttca tgcccatcct cttacagcta
     1681 cagcatttgc atgcgctggt atgagcttag atgagctggt tttggtagaa gggatcctgg
     1741 cattaggatg tgtgcctctt gcacactttg ctcttccggg aacaacagat gtgccggact
     1801 ccatagcacc ttactgcaaa aattataatg cagtactttt agccaatcat ggggctctca
     1861 cctggggcac cagcctaatc caggcatact atcgtatgga aacaacggaa tattatgcaa
     1921 agatcctgat gatgaataat tttattctgg gaaagaaagc acttctctct cagcagcaga
     1981 tcgacgatat tatcgccatc cgcacccgga tgggagttac cagcggtgga agaccaacag
     2041 gaccggaaat ggcatctaat ttagaagatg tgtgtttatc agctcccgga aaagaaaagt
     2101 aaatgagtga aaatgctata cagaaataaa gacagcatct gtttctgtat agcatttttg
     2161 aaatattctg aaaatataca ttttaaattt gtttataagc attaaaatat attaaaaata
     2221 taaataattg taatcaatag atacaattca cgaatatgct aaaaatatag aattaatttt
     2281 aaaatagtat tgacaaaatg cgagatatct ggtatattaa agacaacaaa agaacgaagg
     2341 aaataaataa aaaagaaatt gtagacaatc agtttcgaaa aaatttaaga aaaggaggta
     2401 cggaccgcca gaaagataac attgtttcat ttataaaaga cgtgatttat aaatgaaacc
     2461 aggacaacct caaatcttta aaaaccgctt atttgagaac ctatccacaa gagtatcaaa
     2521 aagtgaaaag tacaaagatt tggaaaatcc ggtttcggtt tggtcgtatg acgagaaagt
     2581 atgatcagga cgaaagagag aataccagat agaaagaaat ataatataaa taagaagtaa
     2641 agaaataaag cggaagaaaa agagaatgag gattacaact gaatatagag agaaagaaac
     2701 agaaacgaaa gtttcaaatg tttcagaaaa gagaggctag tccaatggac gagatagtta
     2761 agagacggat atcgtaatcg cgaagtggtt aatcaaagat tcgatatccg tcttgttttt
     2821 tgtcatttta cagcccttcc agattaaaag gcggtgtgta ttcttctttc gcactgcttt
     2881 tttgctgcat aaaaccgtca gtcatgccaa gctccagggc agtatctact actttgttat
     2941 actcataaga agtgatccgg cggtttagct ggggatagtc cggatttttg gtataaggag
     3001 tgtactggct taacagactt atcagatagg aatgctccgg aaaatgttgg tggatccagt
     3061 aaagcaaccg gatagagtca tctttatgtc cgggaaggac catgtggcgg ataatgacgc
     3121 ctttcgtcat taagggaaaa tccggtctgc cggggacaga ggaaggatgg agcgtatatt
     3181 ctggtgcgcc ggtctggtgg atcatctggg cgatagaagc agaggcctgt tcaaagtaat
     3241 cttttgcttt tgaatagcga aaagccagtt cattgtcaaa atatttaaaa tcaggaagcc
     3301 agatattaat ataatctttt agctgggaaa tgatctccgg gcgttcatag ccgccacagt
     3361 tgcagactac agggatatga agcttgtggc ggaccagatc cagtgcttta ataatatctg
     3421 gaaaaaactg ggtaggcgtt accagattga tattgtaagc gcctttgtcc tgaagacgca
     3481 aaaaggcatc tcccagttcg tttacagtca gttttttacc aaaatggtct ttgcttagta
     3541 gtgagttctg gcagaaacag cagcgtaagg tacagccgga gaaaaagaca gcgccgctgc
     3601 cgcctggttc ttcttccggt ccgctgagca ccggttcctc ccatttatga agggcggctc
     3661 tggccgctga aatgtgactg gagcagccgc agtagcctat ggtcacactg cggtttacat
     3721 ggcacattct gggacagagt gtacagtttg tatagtcagg taagttcatg tgtataaatt
     3781 cctttttgta tttatggttc ggttttgatc agccggtgct gctctctgta ttctttcggt
     3841 gagatcccgt attctttttt aaaagcacgg aaaaagctgg tgtaatcttt aaaaccatat
     3901 tgtggaaaaa tacggctgat aggttcgccg gagaggatag cctggcggct ggcatgaagc
     3961 cgctttttta atatatactg atgcagggaa attcccatat tgtctttgaa aatgtgggca
     4021 atgtggtatt tgctcacata aaaatttgct gcgatccgtt ccagacttaa atcatcttcc
     4081 agattctgat ggatatagtc acatattttt aagtaaaggg cattttcaaa tacctgggaa
     4141 tgttcgtgga gactgctgta aaggagccgg ttaatgtata caaggaaaga catagccatc
     4201 agttcgcttg cctgggcatg aaaaatacgc tcagaactat tttcctctaa cagatccagc
     4261 agctttccct ggatatcctg ggctgtaata cggtcagtag gaatgcggta gatctggtta
     4321 gaggcagcag tgtcaaagca ataggtcagg tcagcatcct gtttatggag tttttcataa
     4381 taagaccggt tcagccagag tacaaaacgg cggtatggct ggtcaaagga gagaaattct
     4441 ggaaaatggg ggattcccgg tggaatcagc aggcagtcac cgtaggaaag tttgtatatg
     4501 gcattaccga tgtggtattt gacatttcct tccagaaaaa aataaaattc gcagtaatca
     4561 tggtggtgag aggcaacaga gcgcagctca gtatcattat aataaaaaag ctcaaaatct
     4621 accggtcgca tgtattgccg tttattgaaa tccatggaaa tctgtttcac gtaaaaatgc
     4681 cctcctgtat agagtaatgt acttggattc tttctgtact tacgcaatat caggcacaaa
     4741 aaaatccgga atgcataatg gtatttttac ggattgtaca taatattgta cgctgtactt
     4801 tggcagtcag ctatattatg gtaactgtga ggaacatctc cgcgaaattg gataccatca
     4861 cctttgttca ggatgaattc ttctccgtca tttataatct tcacagaacc ttctgtaaca
     4921 gtaaaaaatt caatagttcc ctgtaaatgg ggttcggaat tccaatggca gtctttgtcc
     4981 atttccaggt aaaaaacaga aaaacggcgg tttttatcgt cagaaaaaac agaatagttc
     5041 cttgcagtgc catctgcaat aggaattggt tcaatctcag aaatcttaac tacatggttc
     5101 attgggtccg gtcgtcttgt taattcatca aaaggtaccc caagtccatt ggcaatcttc
     5161 cagagaatcc ccaaagtggg atttccgtct cctttttcaa tcagtgccag catacttttg
     5221 ctgactccgc tgagttttcc aagttcctct aagcttaaac gatttttttt gcgcaatata
     5281 gctatattgt tggcaacaat ttcgttgatt ttttccaaaa caaaaaatcc tcctatctat
     5341 tgacaatata ttggagaaaa agtacaatat attatacatt gagtttgcaa acccaacaac
     5401 tgtattatat atcattcatt agaaaaagga aaggatgtgc tgtgtctgaa agtctgtcat
     5461 tttccaatcg cctgttctaa tatattggac atgttttaca gaatcagcac agacaggtat
     5521 gttgtatgaa atatgatttt gacagttgca ttgatcgtac caaggttatg tctattaaat
     5581 ggtcatccaa gcttcgtcag gagatgtatg gagagagcga tgtgatccca ctgggtattg
     5641 cagatatgga ttttaaggca gctccggcag tggcaaaagc gatccaggac cgtgcggctc
     5701 atgagaatta cggatatggg tatcttgcgg atgaatttct catggcctgc gttaactggc
     5761 agaaaagacg taatcactgg gatattaaaa aagaatggat cacatataca ccgggattaa
     5821 atcttccact tgtatttgcc attgaactgt tcacggatcc gggagataac gttatcatcc
     5881 aatctccggt ttattatcct tactatgatt atgttaataa aacaggacgt catattgtat
     5941 acaatgccct tgtaaataaa gacggttatt atgagatgaa tttcgagcag ttggaaatgc
     6001 aggcaaaaga tccgaaaaca aaactgatcc tggtatgcag ccctcaaaat ccaagtggac
     6061 gcctttggac aaaagaggag ctgacccgca tgggaaatat ttgtattgat aatggcgtga
     6121 aggttgtagt agatgagatc cattcagatc ttatcctgac agacagggaa tttgtatctt
     6181 ttgcaacgat cagtgataaa tttgcacaga acagcattat ctgcacgtca ccgtctaaaa
     6241 gctttaatgt agccggactg ctggtatctg atatcattat tccaaatgat gagatgcgtg
     6301 cagcattcca ggaaaaaatg cagccatatt atttatggcc gggtgcattc ggcgcggtag
     6361 cacagatcgc agcttataat gaaagtgagg agtggctgga agaattaaaa gaatatttac
     6421 ggggtaatgc acagtttata gcagatttta tccagaaacg gatgccaaag gtgaaatatc
     6481 gtattccgga agcaacgtat cttggctggc tggatttcag ggcttatgga atgacagatc
     6541 aggaattatg ggattttatg tgccataaag caaaagtggc aacagatgat ggacctaaat
     6601 ttgggcctaa tggggaagca ggcggctttc agcgtattaa tttctcatgc ccgagagcac
     6661 agcttgaaga ggctttgaac cggatcgcag aggcacttga tgctttataa atgtagggag
     6721 gagaagaaag aatgaatgat cctgatatcc ttaaactggt aaaagagcag gaagattacc
     6781 tgatcaaaat gcgcagatat tttcatatga acccggaact gtcaaataag gaatttgaaa
     6841 cacgcaagac gatcatcagt gaacttgaaa aaatgggagt ggagtaccgc ctggctgccg
     6901 ggacggggct ggtagcaatt ataaaaggtg gaaaaccggg aaaacatcat ctgctccgct
     6961 gcgatatgga cgctcttccg gtaaaggaag atactgataa tctgatccag cctaaagcgt
     7021 gtgtttccca gaaacctgga gtatgccatg cctgcggtca tgatgcacat atggctatga
     7081 tgctgacaac gataaaagta ttatctcagg tacgtgaaca attaaagggt acagtatatt
     7141 gctgctttga agaaggggaa gaagcccttg atggttatca gcatgtattg gaactggtga
     7201 gtgagtatcc aattgatgac tgctttgctc ttcatgttta taatgacatg ccggcaggaa
     7261 agattaacct ggtttccgga ccgagaatgg cgggggatgt ttgttttgga gtacatgtga
     7321 tcggaaaaag cggacatggt tccagaccgg atctggcaaa gaatcctgtg atcccggcaa
     7381 gccatattgt ttgtgaattg gattcagctt tacaaaatca gatgaatgcc gaggaaacac
     7441 ttaccttcag tatctgcgta cagaaggctg gtgagatctg gaatgcgatc ccggatgagg
     7501 catttttcag tggaacttcc cggtatttca gccgcccagc aggagaaaag gtgctggcgt
     7561 tgatcaagaa gacctgtaca aacgtggcag atgtacatgg atgccgggtg gagtttgaac
     7621 catatacaaa ggttttgatg gagccggtca ttaatgatca ggaagttgta gaagaaacag
     7681 cacaaaaact gacacagatg tgtggtcccc aggtgttagg agaacatggc ctatggtttg
     7741 cttctgaaac tttttgcaag tatttaaaca aatatcctgg cgcattggga tttttgggaa
     7801 ttgcaaatcc agatctggga tctggcgcag cccatcataa cagccgtttt gatgtggatg
     7861 aatcggcatt actgttagga gtttgtgcag aactgtcatt tgcacttcga tgattatttt
     7921 tacagatatg ctcctggaag cctttgcact taattaagtg agaagctttt gggagtacat
     7981 acaagaaaga aggggattta tgtataattt catgctggca tttttgatct gctgtgtttc
     8041 ttatattata ggtgaaatgg tttctaattt aaccaaagcc tgggttccgt ctgtttttgt
     8101 aagcgctgtg gtcatgcttt tgggatactg gacggtattt ccacacgatc ttgt
//
LOCUS       DBMDUA010000127         8151 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_3949_length_8151_cov_2.657516, whole genome shotgun sequence.
ACCESSION   DBMDUA010000127 DBMDUA010000000
VERSION     DBMDUA010000127.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8151)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3949_length_8151_cov_2.657516"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="ACZPCL_09585"
     CDS             complement(<1..901)
                     /locus_tag="ACZPCL_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1162753.1"
                     /translation="MLNLTKFAVRKPVTIIMCLLTIAYFGIQSLLGTKVELTPEMEFP
                     MLVVSTVYAGASPDDIKDLIATKQEDAVSSLDDVKNVYSFSQDNVAIVLVEYNYGTNI
                     DTAYINLKKAIDGIKSDLPDDANEPNIMEMDMNSQPVITLAVGGQVDGNLYTYVENNI
                     KPELEKLGSVGEVSLAGGQKEYISIKLDPEKVAQYGLSMSRIAQFVGAADFTIPAGDV
                     NVGKQNLSVSVGNDFDNTEALKNVAIPLANGDVIHLSDVATVSNALEDADSIGRYNGQ
                     DIVSVSIKKQQSSTAIDVSKDVLK"
     gene            complement(905..2041)
                     /locus_tag="ACZPCL_09590"
     CDS             complement(905..2041)
                     /locus_tag="ACZPCL_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR1162754.1"
                     /translation="MKKNVKRLIAGAVVLLVAAGIVVRIAGGNKKEEEYETRPTVAVE
                     NPKTGDITLYTDLTGTIEPISKAVVQPKIGGELLEVNFQAGDQVEAGQVLCKIDSDAL
                     TALQLQMQSASVAADNAARELARIQPLYASGYISQQQFDQAQSSATSAQLAYESAKNQ
                     YDLQVEYTTVTAPISGVIESRNVEPHDHIGTDTKICVISGGDQLQVKFGVTEKILRNM
                     KVNDPVQVEKNGTDYEGNVTEIGSMVNSATGLYDAKAFVNNAESLTSGTKVKLTVVMD
                     QVKDVLTVPVDAVNYDNGKAFVYCYDNGAAKKVMIEAGIYDAQTMEVKSGLTAGDQVI
                     TTWSNELVDGQEVLIDSDNGQNENAAQGESAAENAGEETQKAGE"
     gene            complement(2080..2697)
                     /locus_tag="ACZPCL_09595"
     CDS             complement(2080..2697)
                     /locus_tag="ACZPCL_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1162755.1"
                     /translation="MPTQKFINLTESKKQTILTAMCREFMNIPYSKIRISNLIQKAGI
                     SRASFYLYFEDKEDLLGCIAESWVDVILRKLTEAFRVSDGSYYEGMKLWMSQALENDI
                     SREVWKVYKRVMEEEDFTRKSKMGALQFKASPKWRQVIEECYGHMDQSRYPGLTPEKL
                     AYAVDMGLLAMVQLSMRYVEHYAPLAELKEAAWHQLTILDKGIRE"
     gene            complement(2924..3502)
                     /locus_tag="ACZPCL_09600"
     CDS             complement(2924..3502)
                     /locus_tag="ACZPCL_09600"
                     /inference="COORDINATES: protein motif:HMM:NF025636.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1162756.1"
                     /translation="MAAFTKLVIMRVFENLLSVKPLEKITVKDITDQCGISRNTFYYH
                     YQDIYQVFKAYIDYSLEEIFKFLQQENEKDQDHICEKAVEFLENHRTIFENILRSAKS
                     EEVHKILDDGSSRFFRYVIDRVGEGIDSEPEDREMICAMCQHAVGEIMHEWLSRENAD
                     KKSLSDILMRANYLFGGGLERALERSANRFRN"
     gene            complement(3691..5178)
                     /locus_tag="ACZPCL_09605"
     CDS             complement(3691..5178)
                     /locus_tag="ACZPCL_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR1162757.1"
                     /translation="MGKITGFLDYERKTGKALPPKERIKNWNEFHIPLSEEEQKLQGA
                     RCMDCGVPFCQSGVMMCGMVSGCPLNNLIPEWNDLVYTGNWQQAFNRLKKTNCFPEFT
                     SRVCPAPCEAACTCGLNGDPVSIKENEHAIIEKAYEEGYAKVKPPTVRTGKKVAVIGS
                     GPSGLAAADQLNKRGHSVTVFERSDRVGGLLMYGIPNMKLEKWVIDRKVNVMKEEGVI
                     FITNADVGRNYKAAKILRDFDSIILACGASNPRNIDVPGRDAAGIYFAVDYLKANTKS
                     LLDSNLEDGNYISAKDKHVIVIGGGDTGNDCVGTSIRQGCASVTQLEMMPKPPVKRTE
                     NNTWPEWPRILKTDYGQEEAISVFGSDPRIYQTTVKEFVKDGEGKLCKVILVSLTPQK
                     NPETGRTMMVPVEGSEKEVPADLVLIAAGFLGCQDYVADAFGVERNARTNVATEAGSY
                     KTNVDKVFVAGDMHRGQSLVVWAIREGREVAGQVDRSLMGYTNLH"
     gene            complement(5192..>8151)
                     /gene="gltB"
                     /locus_tag="ACZPCL_09610"
     CDS             complement(5192..>8151)
                     /gene="gltB"
                     /locus_tag="ACZPCL_09610"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIR1162758.1"
                     /translation="EEIVTSTTVYVGEEGNILEETPENCKILRVENPILTETDLLKIK
                     NMKKPGFKVEVIPITYYKNTSLEKAIDRLFLEVDRAHKDGANIIILSDRDIDENHVPI
                     PSLLAVSALQQYLVRTKKRTSVALILESGEPREVHHFATLLGYGACAINPYLAHESIH
                     YLIETGMLNKDYYAAVKDYDLAVLHGIVKIASKMGISTIQSYQGSQIFEAIGISKEVV
                     DKYFTDTVSRVGGVTLKDIENDVDVLHSAAFDPLGLDVDLTLDSVGSHKSRSGQEEHL
                     YNPLTIHLLQEATRRGDYSIFKQYTATLDDQGKTYHLRNLMDFNYPEDGGIPIDQVES
                     VDSIVRRFKTGAMSYGSISQEAHETLAIAMNRIHGKSNTGEGGESLDRLVPGPSDHNR
                     CSAIKQVASGRFGVTSRYLVSAQEIQIKMAQGAKPGEGGQLPGKKVYPWVAKTRHSTT
                     GVSLISPPPHHDIYSIEDLAQLIYDLKNSNTRARISVKLVSEAGVGTVAAGVAKAGAQ
                     VILISAYDGGTGAAPRNSIYNAGLPWELGVAEAHQSLIMNGLRDRVVLETDGKLMTGR
                     DVAIACMLGAEEFGFATAPLVTMGCVMMRVCNLDTCPMGIATQNPELRKRFKGKPEYV
                     VNFMKFIAEELREYMAKLGVRTVDELVGRTELLKRKDDIPYSRAKEVDLSRILDNPYE
                     GVKLAGYNEKEVYDFKLDETVDESILLKKLKSALNNGQKKSIQLDVSNVNRTLGTIFG
                     SEITRKYPDGLPEDTFTISCNGSGGQSFGAFIPKGLTLELTGDSNDYFGKGLSGGKLI
                     VYPPQGVQFKAEDNIIIGNVALYGATSGKAFINGIAGERFCVRNSGATAVVEGTGDHG
                     CEYMTGGCVVVLGPTGKNFAAGMSGGIAYVLDEDRSFYKRLNKELVSFEDVSNKYDVL
                     ELKGLIQEHVAYTNSEKGKRILDHFSEYLPKFKKIVPHDYRRMMNAIVQMEEKGLNSE
                     QAQIEAFYVNIRG"
BASE COUNT         2022 a         2058 c         1593 g         2478 t
ORIGIN      
        1 gctttaaaac atcttttgat acatcaatag cagtagaact ctgctgtttt ttaatggata
       61 cagatacaat gtcctgacca ttataacggc caatagaatc tgcatcttcc aaagcattgg
      121 aaacagttgc cacatcagaa agatggatca catctccatt tgccagcggg atcgctacat
      181 ttttcagtgc ttctgtgttg tcaaaatcat ttccgacgct gacagacaga ttctgctttc
      241 ctacgtttac atcacctgca ggaatggtaa agtctgcagc acctacaaac tgtgcaatac
      301 gtgacatgct aaggccatac tgcgctactt tttcaggatc cagcttgatg ctgatatatt
      361 ctttctggcc acctgccaga gaaacttcac caacagaacc taatttttcc agttccggct
      421 tgatattgtt ttctacatat gtataaaggt ttccgtctac ctgaccacct acagccagtg
      481 tgataacagg ctgggagttc atatccattt ccatgatatt tggctcattt gcgtcatcag
      541 gcaggtcact cttaataccg tcgatcgctt tctttaagtt gatgtatgca gtatcaatgt
      601 tggtaccgta attatattca actaaaacga tggctacgtt atcctgtgag aaggaataaa
      661 cgtttttaac atcatccaat gacgaaaccg catcttcttg tttggttgcg atcaggtcct
      721 tgatatcatc agggcttgct cctgcgtaaa cagtgctgac aaccagcata gggaattcca
      781 tctcaggagt cagctctacc ttggtcccta acagggactg gataccaaaa tatgcaatgg
      841 tcagcaaaca catgataatg gtcacaggct tgcgtaccgc aaattttgtc agatttaaca
      901 tcttttattc acctgctttc tgtgtttctt ctccagcatt ttctgcagcg ctttctccct
      961 gggctgcatt ttcattctgt ccattgtcac tgtctataag aacttcctgt ccatctacca
     1021 gctcattgct ccaggttgtg atgacctggt cgcctgcagt cagtccggac ttaacctcca
     1081 tggtctgtgc atcataaata ccggcttcta tcataacctt ctttgcagcg ccattgtcat
     1141 agcagtatac aaatgccttt ccattatcat agtttacagc atctacagga acggtcagta
     1201 catcttttac ctgatccata accacagtca gttttacttt ggttccgctt gtaaggcttt
     1261 ctgcattgtt tacaaatgcc tttgcgtcat acaggccggt tgcggagttt accatactgc
     1321 cgatttctgt tacattacct tcgtaatcag taccgttctt ttcaacctgg accggatcat
     1381 ttaccttcat attgcgaagg atcttttctg taacaccaaa tttaacctga agctggtcac
     1441 caccggagat tacgcagatc ttggtgtctg ttccaatatg atcatgtggt tctacatttc
     1501 tggactcgat cacaccactg atcggtgctg ttactgtagt gtattctacc tgcaggtcat
     1561 actggttctt tgcagactcg tatgccagct gggcgctggt tgcagaagac tgtgcctgat
     1621 caaactgctg ctgtgaaata tagccagatg catacagagg ctggatacgt gccagttctc
     1681 ttgctgcatt gtctgctgct acagaagcgg actgcatctg aagctgtaaa gcagtcagtg
     1741 cgtcagagtc gatcttacaa agcacctgac cagcttctac ctgatcacca gcctggaagt
     1801 ttacctccag aagttctccg ccgatctttg gctgtacaac tgccttggag attggttcaa
     1861 tcgtaccggt cagatcggta tacagagtga tatctcctgt ttttggattt tcaacagcta
     1921 cggttggacg ggtttcatat tcttcttctt ttttgttgcc accggcaata cggacaacaa
     1981 tacccgctgc taccaaaaga actaccgctc ccgcgatgag tctttttaca ttttttttca
     2041 ttttcctgtg ccttcctttc tttttctcaa actttcggat cactctctga tccctttgtc
     2101 taatattgtc agctggtgcc atgcagcctc ctttaactct gccaaaggag catagtgctc
     2161 tacatagcgc atggaaagct ggaccatagc cagcagtccc atatcaacag catatgccag
     2221 tttttccggt gtcagtcccg gatatctgct ctggtccata tgaccataac attcttctat
     2281 gacctgacgc cacttgggac ttgccttaaa ctgtaaagcc cccatcttgc ttttccttgt
     2341 gaaatcctct tcttccatga cccgtttata gactttccat acttctctgg aaatgtcatt
     2401 ttccagtgcc tgactcatcc aaagcttcat gccttcataa tagcttccgt ctgacactct
     2461 aaacgcttct gtcagtttgc gtaatatcac atctacccag gactctgcaa tacaacctaa
     2521 cagatcttct ttatcttcaa aatacagata aaagctggct ctggatatac ccgctttctg
     2581 gatcagattg ctgatgcgga tctttgaata agggatattc ataaactccc tgcacatggc
     2641 tgtgaggatc gtctgcttct tactttccgt aagatttatg aacttttgtg ttggcatcct
     2701 gcttcatctc ctgttcttcc ccaggctgct gtcccaaatg cacactttca tgtgccaaag
     2761 gagaccgggg aaattttgcc ttctctcagt cacaattaat cattatatgg atttttgttt
     2821 aaagagcaat agacaaaata aaaccgaatg cacagttttt gtacattcgg ctattaagtg
     2881 tactaatttg ttaatttatt atcatttatt ttgatacatt ttatcaattc cggaagcggt
     2941 ttgcgcttcg ctctaaagcc cgttccaatc caccgccaaa aagataattg gcccgcatca
     3001 ggatatctga cagtgacttt ttatctgcat tttccctgct taaccattca tgcataattt
     3061 ctccaactgc atgctggcac atagcacaga tcatctctct gtcttccggc tctgaatcaa
     3121 taccttctcc cactcggtca atcacataac ggaaaaaacg gctggaaccg tcatctaata
     3181 tcttgtgtac ttcctcactt tttgcggaac ggagaatatt ttcaaatatg gtcctgtggt
     3241 tttccaggaa ttctactgcc ttttcacaaa tatgatcctg gtctttttca ttttcctgct
     3301 gaagaaattt gaatatttct tccagggaat aatctatata tgccttaaaa acctgataaa
     3361 tgtcctggta atgataatag aaggtattac gtgaaatgcc gcactggtct gtaatgtctt
     3421 ttaccgttat cttctctaag ggtttcacag acaacagatt ttcaaatacc ctcatgatca
     3481 caagtttcgt aaatgccgcc atgtcctgcc gctccttttg tgtccaaagt gtgaacctga
     3541 acttttatta tacgttagag cggcggatat ggcaatagac aaattttgga tttttgtcat
     3601 attggtaacg caaaacaccc cgcctgacgg cggggtgctg ctgcagaatt ggtgttacac
     3661 atccgccaac aggcggaact ctttccatat ttaatgcaga ttggtgtatc ccatcagact
     3721 gcggtctacc tgaccggcta cttctcttcc ctctctgatc gcccatacaa ccagtgactg
     3781 gcctctgtgc atatctccgg ctacaaagac tttgtctaca ttggtcttgt agcttcctgc
     3841 ttctgtggct acattggttc ttgcattgcg ctctactcca aaagcatcgg ccacataatc
     3901 ctggcatcct aagaaaccgg ctgcgatcag taccagatct gcaggcactt ctttttcact
     3961 gccttctacc ggcaccatca tggtacggcc tgtttccgga tttttctgcg gtgtaagact
     4021 gactaagatc accttgcaaa gctttccttc cccgtctttt acaaactcct ttacagtagt
     4081 ctggtaaata cgtggatcag agccaaatac gctgatggcc tcctcctgac cgtagtccgt
     4141 ctttaggatc cgtggccact ccggccatgt attattctca gttctcttta caggtggctt
     4201 tggcatcatc tccaactggg tcacagaagc gcagccctga cggatggaag ttcccacaca
     4261 gtcatttcct gtatcaccgc cgccgatgac aattacatgt ttgtctttgg cagagatata
     4321 atttccatcc tctaaattgg agtctaaaag gctctttgta tttgccttca gataatccac
     4381 tgcaaaatag ataccagcag catctcttcc aggaacatca atatttcttg gattagaagc
     4441 gccgcatgcc aggataatgc tgtcaaaatc acgaaggatc ttggctgcct tataatttct
     4501 tcccacatct gcattggtaa taaagataac tccttcttcc ttcattacat tgactttacg
     4561 gtcgatgacc catttctcca gcttcatatt tgggatgcca tacattaaca ggccacctac
     4621 acggtcactg cgctcaaata cagttacgct gtgtcctctt ttatttaact ggtctgctgc
     4681 tgccagtccg gaaggtccgg aaccgatcac ggccactttc ttaccggtac gcactgtagg
     4741 tggttttacc tttgcatagc cttcttcgta tgccttttca ataatagcgt gctcattttc
     4801 cttaatagat accggatcgc catttaaacc gcaggtacat gcagcttcac agggtgcagg
     4861 gcatactctg gaggtaaact ccggaaaaca gttggtcttt tttaaacggt taaatgcctg
     4921 ctgccagttt cctgtatata caagatcatt ccactccggg atcaggttat ttaaaggaca
     4981 gccgctgacc atgccgcaca tcatcacgcc ggactggcag aagggaacgc cgcagtccat
     5041 gcagcgtgca ccctgtaatt tctgttcctc ttcacttaat ggaatgtgga attcattcca
     5101 gtttttaatg cgctcctttg gtggaagcgc ctttccagtt ttgcgttcat aatctaagaa
     5161 tcctgtaatc tttcccattt ttgacgcctc cttaacctct gatgtttaca taaaatgctt
     5221 caatctgtgc ctgctcactg tttaagccct tttcttccat ctgcacaatg gcattcatca
     5281 tgcggcggta gtcgtgaggt acgattttct taaacttagg cagatactcg ctgaaatggt
     5341 ctaagatacg ctttcctttt tcggagttgg tgtatgccac atgctcctgg atcaggcctt
     5401 tcagctctaa tacatcatat ttattggaga cgtcttcaaa ggaaaccagt tccttgttca
     5461 aacgcttgta aaagcttcta tcttcatcta atacataggc aataccgccg ctcataccag
     5521 ctgcaaaatt ctttcctgta ggtccaagga ctactacgca gccgccggtc atatattcac
     5581 agccatgatc acctgttcct tctactactg cagtagctcc ggagttgcgg acacagaaac
     5641 gttcaccggc aatcccgttg ataaatgcct tgccgctggt agcaccataa agtgcaacgt
     5701 ttcctattat aatgttatcc tctgccttaa actgcacacc ctggggcgga tatacgatca
     5761 gcttgccgcc ggataagcct tttccgaaat agtcattgga atcaccggtc agttccagag
     5821 tcaggccctt agggataaat gcaccgaagc tctggccgcc gctgccgttg cagctgatgg
     5881 taaaggtgtc ttccggcaga ccatccggat actttctggt gatctcagaa ccgaaaatag
     5941 tacctaaagt acggtttaca ttacttacat ccagctgaat gctctttttc tggccattat
     6001 ttaaagcgct ctttaacttc tttaataaaa tgctttcgtc tactgtctca tctaacttaa
     6061 agtcatagac ctctttttcg ttataaccag ccagctttac gccctcatat gggttatcca
     6121 ggatacggct taaatccact tcttttgcac ggctgtatgg gatatcatct tttctcttta
     6181 acagttcagt acggcctacc agttcatcta cagtacgcac acccagtttt gccatatatt
     6241 cacgcagttc ttctgcaatg aatttcataa agtttacaac atactccggt ttacccttga
     6301 aacgcttgcg aagttccgga ttctgggttg caatacccat tgggcatgta tccagattac
     6361 atacacgcat cataacgcag cccatggtta caagaggagc cgttgcaaat ccaaattcct
     6421 ccgcacctaa catacaggca atagcaacat cacggccggt catcagctta ccatctgtct
     6481 ccaggaccac tctgtcacga agtccattca tgatcaggga ctgatgtgcc tctgcaacac
     6541 caagctccca tggaagacct gcattgtaga tagagtttct tggagctgca ccagtaccac
     6601 cgtcataggc agagatcagg ataacctgtg cacctgcttt tgcaacacct gctgcaaccg
     6661 tgccaacacc agcttcagaa accagcttta cagaaatcct tgctcttgta ttagagttct
     6721 ttaagtcata gatcagctgt gccagatcct cgatggaata aatatcgtga tgaggcggcg
     6781 gggagatcag gcttacacca gtagttgaat gtcttgtctt tgcaacccac gggtatacct
     6841 tctttccagg aagctgtcct ccttcaccag gttttgcgcc ctgagccatc ttgatctgga
     6901 tctcctgggc acttaccaga taacggctgg taacaccgaa acggccggaa gctacctgct
     6961 tgatcgcaga acagcggttg tggtcagaag gtcccggtac cagacgatcc aggctttcac
     7021 caccttcacc ggtatttgac ttgccgtgga tccggttcat ggcgatcgcc agtgtctcat
     7081 gtgcttcctg ggaaatagaa ccataagaca tggcaccagt cttaaaacgg cgtactatag
     7141 aatctacact ttctacctga tcgataggaa tacctccatc ttccggatag ttaaagtcca
     7201 taaggtttct cagatggtag gtctttccct gatcatccaa cgtagcagta tactgcttga
     7261 aaatgctgta atcgcctctt ctggttgctt cctgaaggag atggatagta agaggattgt
     7321 ataaatgctc ttcctgaccg gaacgtgact tatggcttcc tacactgtcc aaagtcaggt
     7381 ctacatccag tcctaacggg tcaaaagctg cagaatgaag cacatccaca tcattttcaa
     7441 tatcctttaa tgtaacaccg ccaacacggc ttacagtatc cgtaaaatac ttatccacta
     7501 cttcctttga aataccaatt gcttcaaaga tctgggatcc ctgataagac tggatggtgg
     7561 aaatacccat cttggatgcg atctttacaa tgccgtgaag cactgccaga tcgtagtctt
     7621 ttactgctgc ataataatct ttgtttaaca taccagtctc aatgagataa tggatggact
     7681 catgagccag atacgggttg atagcacagg cgccgtaacc taacaaagtt gcaaaatgat
     7741 gaacctctct tggctcgccg ctctctaaga tcaacgctac acttgtacgc ttcttggtgc
     7801 gtaccagata ctgctgaagg gcagaaacag ccagtaagga tgggatcggc acatggtttt
     7861 catcaatatc acggtcagaa aggataatga tattggcacc gtctttatgt gcacggtcta
     7921 cttccaggaa cagacggtca atggcctttt caagggaagt gttcttataa taagtgatgg
     7981 ggatcacttc taccttaaaa ccaggttttt tcatgttttt gatctttaac agatcggttt
     8041 ctgtaaggat cggattttct acacggagga tcttgcagtt ttccggtgtc tcctccagga
     8101 tattgccctc ttcacctaca tatacagtag tggaagtaac gatctcctca c
//
LOCUS       DBMDUA010000128         8040 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4008_length_8040_cov_2.213125, whole genome shotgun sequence.
ACCESSION   DBMDUA010000128 DBMDUA010000000
VERSION     DBMDUA010000128.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8040)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8040)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8040
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4008_length_8040_cov_2.213125"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="ACZPCL_09615"
     CDS             complement(<1..792)
                     /locus_tag="ACZPCL_09615"
                     /inference="COORDINATES: protein motif:HMM:NF015649.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR1162759.1"
                     /translation="MGEKKLYVNLLGEFSVWYGDKKVYMGRTSTGRQAQLFQLLMISP
                     GFQISKEKLMADIYKSGEFNNRNNSVNNAVYRLRKMLTDAGVPGDEYIAIENGNCVWK
                     EDIPVQVDAVEFKRLLEESRDAEDDDKLHILQEAWLLYRGPLLASNSIEEWAIPENLH
                     YKELYRDCTNQIADILKEKERWEDLYTLYTTALGIETYEEWQLGQLDALIALGEYEKA
                     YKTYEKAVRIYTEVSDLLPPVVAVVCRVYFVLFFEVYAQEIPHVLV"
     gene            complement(863..1045)
                     /locus_tag="ACZPCL_09620"
     CDS             complement(863..1045)
                     /locus_tag="ACZPCL_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162760.1"
                     /translation="MTYLELVAAVGSVPMDIACMYFNGRLTEREMKNVIGWKKAGLVE
                     CFYLQNRNDENNQIRK"
     gene            1233..2057
                     /locus_tag="ACZPCL_09625"
     CDS             1233..2057
                     /locus_tag="ACZPCL_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162761.1"
                     /translation="MDTTKKIIIGAIGAAVVVGAVSIGLIFGLAPADTDLSTIHTQEA
                     TSVPEATEPATVEESSSVQESETVKEGTTGISVQLETYSSGNISIQYPVVSQMNDSAK
                     QDNVNELLKTNALSIIQANDLDETADTLTVKCEVISADRKRLTAVYKGDRMSNGAAYP
                     VSVFYTNTVDLNQVRNLGLSDYTDGYTMAGYVLSDDVEFVGITQDQKEAFLEYRDSLD
                     LDILTEVFNGADFPLASDTVWPESFSYENHGTICFSVPVPHALGDYVIVAFDPSTK"
     gene            2159..2788
                     /gene="upp"
                     /locus_tag="ACZPCL_09630"
     CDS             2159..2788
                     /gene="upp"
                     /locus_tag="ACZPCL_09630"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR1162762.1"
                     /translation="MENVIVFDHPLIQHKISILRKKSTGTNEFRALIEEIAMLMGYEA
                     LRDLPLEDVEVETPIETCMTPMIAGRKLAVVPILRAGLGMVNGILALVPSAKVGHIGL
                     YRDEETHEPHEYYCKLPDPIEERTIVVTDPMLATGGSAVAAVNFIKQHGGKKIKFMAI
                     IAAPEGLKKLQEAHPDIQIYVGHLDRCLNENAYICPGLGDAGDRIFGTK"
     gene            complement(2956..3687)
                     /locus_tag="ACZPCL_09635"
     CDS             complement(2956..3687)
                     /locus_tag="ACZPCL_09635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR1162763.1"
                     /translation="MKLKEMKIKELFDTSHTIAERIFDNHTYAWEVLPEIGDFIRSLG
                     PLLPENEYRKIGTDIWVHKSAKVAPTIMMAGPMIICEGANVRHSAFLRGNVIIGAGAV
                     VGNSCELKNALIFDEAQIPHFNYVGDSVLGYKAHMGAGAVTSNVKSDKSLVVVHAEDK
                     DVATGFKKFGAILGDGVEVGCNSVLNPGTVVGKESNIYPLSSVRGCVAAHSIYKSQDN
                     IVAKENREEAPAPKKRATRKPKVVK"
     gene            complement(3693..5765)
                     /locus_tag="ACZPCL_09640"
     CDS             complement(3693..5765)
                     /locus_tag="ACZPCL_09640"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIR1162764.1"
                     /translation="MSKSLYIAEKPSVAQQFADALKIRGRRGDGYIESDEAIVTWCVG
                     HLVTMSYPEAYDMKYKRWSLNTLPFLPKEFKYEVIPSVEKQFSIVKGLLTRPDVDTIY
                     VCTDSGREGEYIYRLVAQMAGVKDKHQKRVWIDSQTEEEILRGIREAKDESEYDNLSA
                     SAYLRAKEDYLMGINFSRVLTLRYGPSMSQYLGTKFTVLSVGRVMTCVLGMVVRRERE
                     IRNFVKTPFYRVLGSFQATAKDGTPVLLDAEWKAVEGSKYFGTPYLYKENGFKEREKA
                     EELIAYLKEEPPLEAKVLSKEKKKETKNPPLLYNLAELQNDCSKMFKISPDETLKVVQ
                     ELYEKKLVTYPRTDARVLSTAVAKEIYKNINGLMRYEPMAGFAKEAMNLGSFKGIAKT
                     RYVNDKQITDHYAIIPTGQGMGNLRGLSPLSEKVYQVVCRRFLSIFYPAAIYQKYSLV
                     LERKKEQFFASFKVLSEPGYLKVADVNLAKKSSIQETFSDEKEGNSAQTDEKNAEIPK
                     VDRTLLLQVLADLKKNDILTIADLKVKEGETSPPKRYTSGSMILAMENAGQLIEDEEL
                     RAQIKGSGIGTSATRAEILKKLVSIKYLSLNQKTQVITPSLLGEMVYEVVDHSIRQLL
                     NPELTASWEKGLTYVAEGSITSDEYMEKLERFVAMRTVNVTRLNNQYDMRGLFDAAAA
                     FYKTKKEN"
     gene            complement(5929..6987)
                     /locus_tag="ACZPCL_09645"
     CDS             complement(5929..6987)
                     /locus_tag="ACZPCL_09645"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR1162765.1"
                     /translation="MAIQKKEFGVMPSGEKVYEYTLTNKSGVSASFIELGATWTKMLV
                     PDRDGNMADVILGYDDLASYQVNTPHFGAPIGRNANRIGNAVITIDGKDYKLEANNGP
                     NNLHSGPDLYHSRLWESWAYETAEGSRLDFFLNSPDGDQGYPGNAKIRISYTLTEDSC
                     LKIDYSMVADADTVANFTNHCYFNLAGHNSGPVMDQQVWLAADTYTPADAVSIPTGEI
                     LPVAGTPMDFTKMKPIGQDIEVDFEALILGHGYDHNWVLNHEAGVLGIAGKAKDPASG
                     RVMEAYTDLPGMQFYTANFLKDERPGKGGAHYDRRHAFCFETQYYPDACHKPNFPSPI
                     LKAGDTYKTTTIYKFYAE"
     gene            complement(7075..>8040)
                     /locus_tag="ACZPCL_09650"
     CDS             complement(7075..>8040)
                     /locus_tag="ACZPCL_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase family
                     protein"
                     /protein_id="HIR1162766.1"
                     /translation="SAGKTYEEAVGDRWKMTTPMPEAVKKLVVMNVNEVEKVAASVDF
                     VFSAVDMTKDEIRAIEEEYAKTETPVVSNNSAHRWTPDVPMVVPEVNPEHFDVIEFQK
                     KRLGTTRGFIAVKPNCSIQSYAPVLTAWMEYEPYEVVATTYQAISGAGKTFKDWPEME
                     GNIIPYIGGEEEKSEQEPLRIWGHIENGVIVKAESPVITCQCIRVPVLNGHTAAVFVK
                     FKKKPTKEQLIEKLVNFKGIPQELELPSAPKQFIQYLEEDNRPQVSLDVDFEHGMGIS
                     VGRLREDTVYDYKFVGLSHNTVRGAAGGAVLCAELLTAKGYIAAK"
BASE COUNT         2033 a         1909 c         1658 g         2440 t
ORIGIN      
        1 aacgaggaca tgcggaattt cctgcgcata aacctcgaag aacagtacga aatatacacg
       61 gcacacgacg gcaacgacgg gtgggagcag atccgaaact tctgtataga tccggaccgc
      121 tttttcatat gttttatatg ctttctcata ctctcctaaa gcgatcagcg catccagctg
      181 ccctaactgc cattcctcgt aagtctctat tcccaatgca gttgtataca gggtatacag
      241 atcttcccag cgttcttttt cttttaatat atccgcgatc tggttggtac agtcacgata
      301 cagttcttta taatgaagat tctcaggaat agcccattct tcaatggaat tgctggcaag
      361 cagcggtcct ctgtagagaa gccaggcttc ctgaagtata tgaagtttat catcatcttc
      421 tgcatcccgg ctttcttcta acagccgttt gaattccaca gcatctacct gaacaggtat
      481 atcttctttc cacacacagt ttccattttc aatggcgata tattcgtcac ctggaacccc
      541 cgcatccgtg agcatttttc tcagacgata gaccgcattg ttgacactgt tgttccggtt
      601 attgaactct cctgatttat aaatgtcagc cattaatttt tccttggata tctgaaatcc
      661 cggactgatc atcagaagct ggaacagctg tgcctggcgg ccggtagatg tacgtcccat
      721 gtacaccttt ttgtctccgt accacacaga aaactctcct aataaattaa catataactt
      781 cttctctccc acatcaacag cccctttgct tttgttcaca cctaattata aatgtagcac
      841 aaggggtatt tattttcaat attcatttac ggatttgatt attttcatca tttctatttt
      901 gcagataaaa acattccacc agaccggcct ttttccaacc gatcacattt ttcatttccc
      961 gttctgtcag cctgccgtta aagtacatac aggcaatatc cataggtacg ctgcctactg
     1021 ctgcaaccag ttctaaatat gtcattttac attccctccc gtttttatcc tgcgtgacat
     1081 aacctttttg acacgctctt atactatatg aaaggaaatg aaaatatatt cctgttatcc
     1141 ttcatgaatt tgtaagtatt tctttataca gagtccataa aagcagagta atatataata
     1201 aggaagaaat actatacgag gaggaaataa ttatggatac aaccaaaaag atcattatcg
     1261 gtgccatagg tgctgctgtt gtggtcggcg cagtttccat cggtcttatc tttggtctcg
     1321 ctccggcgga tacggatctg tctaccatac atacccagga agctaccagt gtcccggaag
     1381 caacagaacc tgcaactgtg gaggaaagtt cttctgttca ggaaagtgag actgttaaag
     1441 aaggaactac aggtatcagc gtacagcttg agacttattc ttccggaaat atttccattc
     1501 aatatccggt ggtaagccag atgaacgaca gcgcaaaaca ggacaatgta aatgaacttt
     1561 taaagaccaa tgccctttcc attattcagg caaacgatct ggatgaaacc gcagatactc
     1621 ttactgtaaa atgcgaagtc atatctgcag accggaagcg tctcactgct gtttacaaag
     1681 gagaccgcat gagtaatgga gctgcttatc ctgtcagtgt tttttatacc aacacagtgg
     1741 atctgaacca ggtaagaaat cttgggcttt ctgattacac agatggctac actatggccg
     1801 gttatgtttt aagtgatgat gtggaatttg taggtataac ccaggatcag aaggaagctt
     1861 ttttagagta tcgtgacagt ttggatctgg atatccttac agaggtattt aacggagccg
     1921 actttccgct ggcatctgat actgtatggc cggaatcctt cagctatgaa aaccacggaa
     1981 ctatctgctt ttccgttccg gtcccccacg ctttgggaga ttatgtgatc gttgcatttg
     2041 atccttctac caagtaatta acgttgccat tatccacgct atcagttata ataggaaaca
     2101 gaaaagacag acaagtatct gtgtcttata caacacgaat aaggagcagc aaaaaactat
     2161 ggaaaatgta attgtttttg atcacccatt gatccagcac aagatctcta ttttaagaaa
     2221 gaaaagcacc ggtacaaatg aattccgcgc tttgatcgaa gagatcgcca tgctgatggg
     2281 atacgaagct ttaagagatc ttcctctgga agatgtagaa gttgagacac caatcgagac
     2341 ctgtatgaca cctatgatcg caggccgtaa gctggcagtt gtgccgatct taagagcagg
     2401 tcttggtatg gtaaacggta tcctggcact ggttccaagt gctaaggtcg gacatatcgg
     2461 tctttacaga gatgaggaga cacatgagcc tcatgaatat tactgcaaac ttccggatcc
     2521 gatcgaggaa cgtaccattg ttgttactga cccaatgctg gcaaccggtg gatctgctgt
     2581 tgcagctgtt aatttcatca agcagcacgg tggaaagaag atcaaattta tggccatcat
     2641 cgcagcaccg gaaggtctta agaaactgca ggaagcacat ccggacattc agatttatgt
     2701 tggacattta gacagatgtt taaatgaaaa tgcgtatatc tgcccaggtc ttggagatgc
     2761 gggagatcgt atcttcggaa caaaatagga cgtaaaaaat taacccccgc tggttagcgg
     2821 gggttttatt atatattctt cactcacgaa ccacgcgctc cactgcccgc tttcgcgggc
     2881 ggttcgttcg ttaatggctt gcaaaaaaca aatgtctgct accgcagccg cttgtttttt
     2941 gctgcgcaca ttacgttatt tcacaacctt tggctttctg gttgctctct tctttggtgc
     3001 cggagcttct tcacggtttt cctttgctac gatattgtcc tggctcttgt agatggagtg
     3061 agcagcaaca cagcctctta cgctggataa tgggtagatg ttgctttcct tacctacaac
     3121 agttcctgga ttaagaacac tgttgcagcc tacttctacg ccatcaccaa ggatcgcgcc
     3181 aaatttctta aatccggtag ctacgtcctt atcttctgcg tgtactacaa ctaaagactt
     3241 gtcagacttt acattggaag taactgcgcc tgcgcccata tgagccttat atccaagtac
     3301 ggagtcacca acatagttaa agtgtgggat ctgtgcttcg tcaaagatca gagcattctt
     3361 taattcacag gagttgccta caacagcgcc agcgccgatg ataacatttc ctcttaagaa
     3421 tgcgctgtga cgtacatttg cgccttcaca gatgatcata ggaccagcca tcataatggt
     3481 aggtgctact tttgcgctct tgtgtaccca gatatcagtg ccgatcttgc ggtattcatt
     3541 ttccggaaga agaggtccta aagaacggat gaaatcaccg atctccggaa gtacttccca
     3601 ggcataagta tgattatcaa aaattctctc agcaattgta tggcttgtat caaacagctc
     3661 tttaattttc atttccttca gtttcattgt aatcagttct ccttttttgt tttgtaaaag
     3721 gcggcagccg cgtcaaaaag tccccgcata tcatactggt tattcaatct ggtcacgttt
     3781 acagtgcgca ttgcaacaaa acgttccagt ttttccatgt attcatccga tgtaatggaa
     3841 ccttccgcca cataagtcag acctttttcc cagctagcag tcagctcagg atttaataac
     3901 tgcctgatgg agtggtccac cacttcataa accatctctc ccaacagaga cggggttatc
     3961 acctgggtct tctgatttaa actcagatat ttgatactga ccagtttttt cagtatctcc
     4021 gctctggtgg cactggtgcc gatgccgctg cccttgatct gggcccgcag ctcctcatct
     4081 tcgatcagct ggccggcatt ttccatagcc aggatcatgg aaccggaggt atatcttttt
     4141 ggtggagaag tctctccttc cttcaccttc agatccgcta tagtaagtat atcatttttt
     4201 ttcaaatctg caagcacctg taacagcaga gttcggtcaa ctttcggaat ttccgcgttt
     4261 ttttcatctg tttgtgcaga atttccctct ttctcatcag aaaaggtctc ctggatgctg
     4321 cttttctttg ccaggttcac atctgccacc tttaaataac caggttctga cagcacttta
     4381 aagcttgcaa aaaactgttc ttttttccgt tccagtacca gactgtattt ctggtaaatg
     4441 gcagccggat aaaagatgct taaaaaccgg cggcaaacta cctgatagac cttttcagat
     4501 aacggggaca gtccgcgcaa gtttcccatt ccctgtcctg taggaatgat ggcataatgg
     4561 tctgtgatct gcttgtcatt gacataacgg gtctttgcga tccctttaaa acttccaaga
     4621 ttcattgctt cttttgcaaa acctgccatt ggttcatagc gcatgagacc gttaatattt
     4681 ttatagatct cctttgcaac agctgttgaa aggactctgg catcagttct cggatatgtg
     4741 accagctttt tctcatacag ttcctggacc actttcaaag tctcatccgg gcttatttta
     4801 aacattttgg aacagtcatt ctgaagttcc gcaaggttat aaagaagagg cggatttttc
     4861 gtttctttct tcttttcttt ggaaaggact tttgcctcta aaggcggctc ttcttttaag
     4921 taggcgatca gctcttctgc tttctccctt tctttaaaac cattttcttt ataaagatag
     4981 ggcgttccaa aatatttaga gccttctacc gccttccact ctgcgtctaa cagcacaggt
     5041 gtaccgtctt ttgcagttgc ctgaaagctt ccaagaacac ggtagaacgg ggtttttaca
     5101 aagtttcgta tttcccgctc cctgcggact accattccca ggacacaggt cataacacgg
     5161 cctacggaaa ggactgtaaa ttttgttccc agatactgag acatagacgg cccgtagcgc
     5221 agggtcagta ctctggaaaa attgataccc ataaggtaat cttctttggc tcttaagtaa
     5281 gcagaggcag aaaggttgtc atattcagac tcatcttttg cttcccggat cccgcgaagg
     5341 atctcctctt cggtctggga gtcgatccac acacgcttct ggtgcttgtc ctttacacca
     5401 gccatctggg ctaccagacg gtaaatatac tctccttccc gtccggagtc ggtgcagaca
     5461 taaatagtat ctacatccgg ccttgtaaga agtcctttta caatggaaaa ctgcttttcc
     5521 acacttggta ttacttcata tttgaattct tttggcagaa aaggaagtgt attcagactc
     5581 cagcgcttgt atttcatatc gtaggcttcc gggtaactca tggtcaccag atgtcccacg
     5641 caccaggtga cgatcgcctc atctgactcg atatagccgt ctccgcgacg tccccgtatt
     5701 ttcaatgcat ctgcaaactg ctgggctaca ctgggctttt ccgcaatgta taatgatttt
     5761 gacatttgat tcctcattat ttaaagacat attttataag tataccattt tttcagggct
     5821 cttgcaagaa gttataaagg acagtccaaa gccattttcc agcaatagac tgtccttttt
     5881 gtactgtata tttttgaaga tctgcctgcc ggcagtatcc gtctgaccct attctgcgta
     5941 gaacttataa atggtagttg tcttataagt atctcctgct tttaagatcg gtgacgggaa
     6001 gtttggctta tggcaggcat ctggatagta ctgtgtctca aagcagaacg catggcgtct
     6061 gtcatagtgg gcaccgcctt ttccagggcg ctcatctttt aagaagtttg cagtataaaa
     6121 ctgcattcca ggaagatctg tgtatgcttc catcacacgg ccgcttgctg gatcttttgc
     6181 cttacctgca atgccaagaa cgcctgcttc atggtttaat acccagttgt gatcgtatcc
     6241 atgtccaagg atcaaagcct caaaatcaac ttcaatgtcc tgtccgatcg gtttcatctt
     6301 tgtaaaatcc ataggagttc ctgctaccgg aaggatctct cctgttggga tggaaaccgc
     6361 gtctgcaggt gtgtatgtat cagctgcaag ccatacctgc tgatccatta cagggccgct
     6421 gttatgtcct gccaggttga aatagcaatg gttggtaaag ttagctactg tgtctgcatc
     6481 tgcaaccatg ctgtaatcga tctttaagca gctgtcctct gttaaggtat agctgatgcg
     6541 gatctttgca tttccagggt atccctgatc tccgtccggg ctgtttaaga agaagtccag
     6601 gcggctgcct tctgctgtct catatgccca gctttcccat aaacggctgt gatacagatc
     6661 cggaccactg tgcaggttgt taggaccatt gtttgcctct aatttataat cttttccgtc
     6721 aatggtgatc actgcgtttc caatacggtt tgcatttctg ccgatggggg cgccaaaatg
     6781 aggtgtattt acctgatagc ttgcaagatc gtcatagcct aagatcacat ctgccatatt
     6841 gccatcacgg tccggaacca gcattttggt ccaggttgcc ccaagttcaa taaacgacgc
     6901 gctgacaccg cttttgttgg tcagtgtgta ctcgtatacc ttttccccgg aaggcataac
     6961 accaaattct tttttctgaa tagccataag aaaacctcct tataattaat ctgtaactaa
     7021 tccaatgtcc ccccggcaaa ataccaggag gacaaaacat acttcttata cttattattt
     7081 agctgcgata tatcccttag ctgtaagaag ttctgcacac agaactgcac cgcctgctgc
     7141 acctcttacg gtgttgtggg aaagtcctac aaatttgtag tcatatacgg tatcttctct
     7201 taaacggcct acagagatgc ccataccatg ttcaaaatct acatccaggc ttacctgtgg
     7261 acggttgtct tcttccagat actggatgaa ctgctttgga gcacttggaa gttccagttc
     7321 ctgaggaatg cccttgaagt ttactaactt ctcgatcagc tgttccttgg ttggtttctt
     7381 tttaaactta acaaatacag ctgctgtatg tccgtttaat acaggaacac ggatgcactg
     7441 gcaggtgatc acagggcttt ctgcctttac gatcacaccg ttttcaatgt ggccccagat
     7501 acgcagtggc tcctgctcgc tcttttcttc ttctccgccg atgtatggga tgatgtttcc
     7561 ttccatttca ggccagtcct taaaggtctt tcctgctccg gagattgcct ggtaggtagt
     7621 tgcaactact tcatatggct catattccat ccatgcagtt aaaacaggtg cgtagctctg
     7681 aatggagcag tttggcttta ctgcgatgaa gcctctggta gttcccagac gctttttctg
     7741 gaattcgatc acatcaaaat gttctgggtt tacctccgga acgaccatag gaacgtctgg
     7801 agtccaacga tgtgcgctgt tgttggaaac aaccggtgtt tctgtctttg cgtattcctc
     7861 ttcaatggca cggatctcat ccttggtcat atccactgca ctgaatacga agtctacact
     7921 ggcagctact ttctctactt cgtttacgtt cataacaacc agcttcttta cagcttccgg
     7981 cataggagtg gtcattttcc agcggtcacc tactgcttct tcgtaggtct ttcctgctga
//
LOCUS       DBMDUA010000129         7974 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4043_length_7974_cov_3.483043, whole genome shotgun sequence.
ACCESSION   DBMDUA010000129 DBMDUA010000000
VERSION     DBMDUA010000129.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7974)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4043_length_7974_cov_3.483043"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /gene="rplL"
                     /locus_tag="ACZPCL_09655"
     CDS             complement(<1..112)
                     /gene="rplL"
                     /locus_tag="ACZPCL_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515516.1"
                     /note="present in two forms; L12 is normal, while L7 is
                     aminoacylated at the N-terminal serine; the only multicopy
                     ribosomal protein; 4:1 ratio of L7/L12 per ribosome; two
                     L12 dimers bind L10; critically important for translation
                     efficiency and fidelity; stimulates GTPase activity of
                     translation factors; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR1162767.1"
                     /translation="MAKLTTAEFIEAIKELSVLELNDLVKACEEEFGVSAA"
     gene            complement(216..716)
                     /gene="rplJ"
                     /locus_tag="ACZPCL_09660"
     CDS             complement(216..716)
                     /gene="rplJ"
                     /locus_tag="ACZPCL_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR1162768.1"
                     /translation="MAKVELKQPVVDEIKAMLDGAVGAVVVDYRGLTVEQDTKLRKQL
                     REAGVSYKVYKNTLIKRAAEGTEFAALDPHLEGPTALAVSKDDATAPARILAEFAKTA
                     PKLELKASVVEGTYYDQAGTQVIATIPSREVLLGKLLGSIQSPITNLARVLNQIAEKN
                     GGAAEA"
     misc_feature    complement(771..908)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1068..1763)
                     /gene="rplA"
                     /locus_tag="ACZPCL_09665"
     CDS             complement(1068..1763)
                     /gene="rplA"
                     /locus_tag="ACZPCL_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR1162769.1"
                     /translation="MKTGKRYAEAMKNVDRAALYDVADAISIVKKNASAKFDETVELH
                     IRTGCDGRHADQQIRGAVVLPHGTGKTVRILVFAKGAKADEAQAAGADFVGAEELIPR
                     IQNEGWLDFDVVVATPDMMGVVGRLGRVLGPKGLMPNPKAGTVTMDVTKAIKEIKAGK
                     IEYRLDKTNIIHVPVGKASFTEEQLADNFQTLMDAILKAKPSTVKGAYFKSVALTSTM
                     GPGVKLNVAKLAN"
     gene            complement(1831..2256)
                     /gene="rplK"
                     /locus_tag="ACZPCL_09670"
     CDS             complement(1831..2256)
                     /gene="rplK"
                     /locus_tag="ACZPCL_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR1162770.1"
                     /translation="MAKKVTGYIKLQIPAGKATPAPPVGPALGQHGVNIVQFTKEFNA
                     RTADQGDMIIPVVITVYADRSFSFITKTPPAAVLIKKACGLKSGSGVPNKTKVAVLKK
                     ADLQKIAETKMPDLNAASLEAAMSMVAGTARSMGVTIEE"
     gene            complement(2337..2852)
                     /gene="nusG"
                     /locus_tag="ACZPCL_09675"
     CDS             complement(2337..2852)
                     /gene="nusG"
                     /locus_tag="ACZPCL_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR1162771.1"
                     /translation="MSEAHWYVVHTYSGYENKVKVDIEKTIENRNLQDQILEVSVPML
                     PVVELKNGVEKKADKKMFPGYVLINMVMNDDTWYVVRNTRGVTGFVGPGSKPVPLTEE
                     EMASLGFMKEDVLVDFTLGDMVTVISGAWKDTVGAIKAINESKQTITINVEMFGRETP
                     VELGFAEVKKM"
     gene            complement(2875..3126)
                     /gene="secE"
                     /locus_tag="ACZPCL_09680"
     CDS             complement(2875..3126)
                     /gene="secE"
                     /locus_tag="ACZPCL_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR1162772.1"
                     /translation="MEENTVNTVETTEKAAKTDQKTAKKEKKPSFFKGLKTEYKKIVF
                     ADKETVAKQTVAVVAMSVFIGALIGVLDMIMKFGLSFIL"
     gene            complement(3153..3317)
                     /gene="rpmG"
                     /locus_tag="ACZPCL_09685"
     CDS             complement(3153..3317)
                     /gene="rpmG"
                     /locus_tag="ACZPCL_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR1162773.1"
                     /translation="MEVEVVRTKITLACTECKQRNYNMTKDKKTHPDRMETKKYCRFC
                     KRHTLHKETK"
     gene            complement(3488..4204)
                     /locus_tag="ACZPCL_09690"
     CDS             complement(3488..4204)
                     /locus_tag="ACZPCL_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR1162774.1"
                     /translation="MNYIIETILDLLFPRRCPVCGQIIMPKGNLICPGCIRKLSWVRG
                     PVCKCCGKEIISETAEYCSDCAIHPRSFSNGMALLNYNEISAPSLAKIKYHNHREYLD
                     FYAEAAYRKYAARIARLSPDLIIPVPVHKSRMKKRGFNQAEEFGKRLSHYLEIPMNSS
                     LLIRSKKTVPQKDLGPALRLKNLEQAFSCRKLPYRMKKVLLIDDIYTTGSTAEACSRA
                     LKKAGAEQVFLLVIATSSNR"
     gene            complement(4280..6568)
                     /gene="recD2"
                     /locus_tag="ACZPCL_09695"
     CDS             complement(4280..6568)
                     /gene="recD2"
                     /locus_tag="ACZPCL_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIR1162775.1"
                     /translation="MATITGFIEKIKFRNEENGYTVLSVTDQSDGDEVIMVGTLSYAA
                     EGDMIQASGRMIEHPVYGEQLQIESYEMKTPQDAESMERYLGSGAIKGIGVALAARIV
                     RHFKADTFRVMEEEPERLSEVKGISEKMAMAIAEQVEEKKGMREAMMFLQNYGITLNL
                     AAKIYQEYGPKLYSIIKENPYKLADDIPGVGFKMADEIAEKVGIFTDSDYRIRAGLLY
                     ILLQASANGHTYLPQKELFSQAAELLKVEPTAMEKHLMDMQIDRKVVVKTVPGSEPPE
                     YLVYSSHYYYLELNTARMLHDLNIRGDIPETEIRANLAKVQKKEEIHLDELQEKAVFE
                     AVNSGLLVITGGPGTGKTTTINAIIQYFENEGMEILLAAPTGRAAKRMTETTGYEART
                     IHRMLELVPSGIPDSGSMSGNNAPSGKNGSFSSGGMHFDRNEENPLDADVIIIDEMSM
                     VDISLMHSLLRAVNVGTRLILVGDVDQLPSVGPGNVLRDIIESGCFNVVKLTHIFRQA
                     AQSDIIVNAHKINEGERVDLTKRSKDFLFIRRENPDAVISAAITLIQQKLPGYVHAQP
                     GDIQVMTPMRKGALGVERLNQILQNYLNPPDPSKKEKESGGIIYRTGDKVMQIKNNYQ
                     MEWEIRSKYGIPTDKGAGVFNGDMGIIREINEYAENLTVEFDEGKMVEYSFKQLEELE
                     LAYAITIHKSQGSEYPAVIIPMYSGPRMLMTRNLIYTAVTRARSCVCLVGRPDAFYTM
                     ADNSMEQKRYCGLKTRIEEIYA"
     gene            complement(6666..7718)
                     /gene="mreB"
                     /locus_tag="ACZPCL_09700"
     CDS             complement(6666..7718)
                     /gene="mreB"
                     /locus_tag="ACZPCL_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR1162776.1"
                     /translation="MFSMMSNDIGIDLGTASILVYIKGKGVVLKEPSVVAIDRDTNKI
                     MAIGEEARLMIGRTPGNIVAVRPLRQGVISDYTITEKMMKYFINKAVGKRTLRKPRIS
                     VCIPSGATEVEKKAVEDATYQAGAREVAIIEEPVAAAIGAGIDIGKACGNMIVDIGGG
                     TADIAVISLGGPVVSTSIKIAGDDFDEALVRYMRKKHNLLIGERTAEEIKINIGAAYR
                     RPEVQTMEVRGRNLVTGLPKTIVVTSDETLEALREPALQIVDAVHNVLERTPPELAAD
                     IFDRGIVMTGGGSLLSGLDALIEEKTGITTVIAEDPLTAVAIGTGKFIEFAHGMTSAL
                     EASMGIGGGRNDKEDY"
BASE COUNT         1921 a         1917 c         1650 g         2486 t
ORIGIN      
        1 ctgctgcaga tacgccaaac tcttcctcac atgcctttac taaatcgttc agttctaata
       61 cggataattc cttgatagct tcgataaact cagcggttgt taactttgcc attttaattt
      121 cctcctgttt aaaatttatt tttcgatagg atctgtgttt caggctgctt tttccggact
      181 atgcggataa aagagccaca aattcatttt gcagattaag cctctgctgc accaccattt
      241 ttctctgcga tctgattgag tacgcgagcc aggttggtga ttggggactg gatgcttcca
      301 agcagctttc caagaagaac ttctctggat ggaatggttg cgataacctg ggttccagcc
      361 tgatcatagt aggttccttc aactacagaa gccttcagtt ccagcttagg agcggtctta
      421 gcgaattcag ccagaattct agctggtgct gtagcatcat ccttagatac agctaatgct
      481 gtaggtcctt ccagatgtgg atccagagct gcgaactcgg ttccttctgc tgcacgcttg
      541 atcagagtgt tcttatatac cttataagaa actccagctt ctcttaactg tttacgcagt
      601 tttgtatcct gctctacagt cagaccacgg taatcaacaa caactgcacc tacagcgccg
      661 tccagcattg ccttgatctc gtctactact ggctgcttta attcaacttt tgccatgaat
      721 ggtttacctc cttatattat ttcgtattga aaaccgtcat agcaaggaaa cgatcttata
      781 aaaaacctcc ctgccgcaat gacagagagg tatgcaaaat ctcattagag ctttgtatgt
      841 tcctcggcag gcgttttaag cttacacctt acggtacctg ctgtctcagg gcattcaaca
      901 cgcataataa aataacatat ctgtgcatga atgtcaagag atttttgcaa caaattgtat
      961 ttttttaact tttttcacct ccagccatgg gcgggagaac acgaaagcaa aaaggccaca
     1021 gagaaccaaa attctctgca gccttatttt ctattcagga cacctgatta gtttgcaagc
     1081 ttagctacgt tcagcttaac gcctgggccc atggtggatg taagagctac gctcttgaag
     1141 tatgcgccct taacggtgct tggcttagcc tttaagatag cgtccataag cgtctggaag
     1201 ttgtccgcta actgctcctc ggtgaaggaa gcttttccta ctggcacgtg gataatgttt
     1261 gtcttgtcaa gtctgtactc gatcttacca gctttgattt ccttgatagc cttggttacg
     1321 tccatggtta cggtaccagc ctttgggttt ggcattaagc ccttaggtcc aagtacacga
     1381 cccagacgac caacaacgcc catcatgtca ggagtagcaa caacaacgtc gaaatccagc
     1441 catccttcgt tctggattct tgggatcagt tcctcagcgc ctacgaaatc tgcgccagct
     1501 gcctgtgcct cgtctgcctt tgcaccctta gcgaatacta agatacgaac agtcttgcct
     1561 gttccgtgag gaagaacaac agctccacgg atctgctgat cagcgtgacg tccgtcacat
     1621 cctgttctga tatgaagttc tacagtctcg tcaaattttg cagaagcgtt cttcttaacg
     1681 atagagattg catctgctac gtcgtaaagt gcagcacggt ctacgttctt catagcctct
     1741 gcatatcttt ttcctgtttt cattataaaa taacctccta gtggtattga cgggggaatc
     1801 tccctcccac gttcatatat acggtattaa ttactcttcg atggtaacgc ccatacttct
     1861 tgcagtacca gctaccatgc tcatagcagc ctcaaggcta gcagcgttta agtctggcat
     1921 cttggtctct gcgatcttct gcaggtcagc cttctttaat acagctacct tagtcttgtt
     1981 aggaacacct gaaccgctct ttaaaccgca agccttcttg atcagaactg ctgctggcgg
     2041 ggtcttggtg atgaagctga agcttctgtc cgcatatacg gtaataacta cagggatgat
     2101 catatctccc tggtctgctg ttctagcgtt gaactctttg gtaaactgta cgatgtttac
     2161 accgtgctga ccaagagctg gtccaactgg tggtgctggt gtagccttgc ctgctggaat
     2221 ctgtaatttg atatatcctg ttactttctt tgccatctta ggcacctcct aaaattatgt
     2281 ggtattgtcg ggaatgtccc tcccaccagc ttctgctgcg caaaagctga tccattttac
     2341 attttcttta cttctgcaaa tcccagttcg accggtgttt cacgaccgaa catttctaca
     2401 ttgatagtga tggtctgttt actttcgttg atcgccttaa tagcaccaac tgtatctttc
     2461 catgcaccgg agataactgt taccatatct cccaatgtaa agtcgactaa gacgtcttcc
     2521 ttcataaatc cgaggcttgc catttcttct tctgtcagag gaactggttt agagcccgga
     2581 ccaacaaagc ctgtaacgcc tcgggtgttg cgtactacgt accatgtgtc atcgttcatg
     2641 accatgttga tcagcacata acccggaaac attttcttgt cggctttttt ctccacgcca
     2701 ttcttcagtt ctacaacagg aagcatgggc acggatacct caaggatctg atcctgaagg
     2761 tttctgtttt caattgtctt ctcaatgtcg accttgactt tgttttcata acctgagtag
     2821 gtatggacta cataccaatg tgcttctgac atacaatcac cctcgccttt gaaattaaag
     2881 aataaagctt aaaccgaatt tcataatcat atccagtaca ccgatcagag cgccgatgaa
     2941 aacggacatg gctacaactg caacagtctg ttttgctaca gtttccttat cagcaaagac
     3001 gatcttcttg tactctgtct tcaggccttt gaaaaagcta ggctttttct ccttcttagc
     3061 cgtcttctgg tcagtcttgg ccgctttttc cgtggtttct acagtattca ctgtattttc
     3121 ttccataact tcacgtcctt tactgatcag gattactttg tttccttgtg taacgtatgt
     3181 ctcttgcaga atctgcagta cttcttagtc tccatgcggt caggatgagt tttcttatcc
     3241 ttagtcatgt tgtagttacg ctgcttgcat tctgtacatg ccagtgtgat ttttgtgcgc
     3301 acgacttcca cctccattaa attatcgtaa ttcaatttgc ttctcggtct taattttaag
     3361 cataaaaaaa agacctatct tccattcgcc cgtatactat aacataaaaa tattcctgac
     3421 gtcaagaggt ataaaaggtc ttttctatag tttttaatga tttttatcat tttatacaag
     3481 atttttgtta tcgattggag ctggtggcga tcacaagaag aaaaacctgc tctgctccgg
     3541 cttttttcaa agcccttgag caggcttctg cagtacttcc agttgtgtaa atatcatcaa
     3601 taaggagaac ttttttcatt ctgtacggca gttttctgca gctaaatgcc tgttccagat
     3661 ttttaaggcg cagggcaggt cctaaatctt tctggggtac cgtctttttt gaccggatca
     3721 gcaaactgga attcattgga atttccagat aatgggacag acgttttcca aattcttctg
     3781 cctgattaaa gcctcttttt ttcatgcggc ttttgtgtac cggcaccgga atgataagat
     3841 ccggtgataa gcgggcgatc ctggcagcat actttctata tgcagcctct gcataaaaat
     3901 ccagatattc ccgatggttg tgatacttga tctttgccag ggacggtgct gaaatttcat
     3961 tatagttaag cagcgccatc ccattggaaa aagatcttgg atgaattgcg cagtcagaac
     4021 agtattctgc ggtctcgctt atgatctcct tgccacagca tttacacacc ggtccacgga
     4081 cccaggacaa ttttctgata cacccagggc agatcagatt tccttttggc ataatgatct
     4141 gaccgcaaac cgggcacctg cgcgggaaca gcagatccag gatcgtttct attatatagt
     4201 tcatacgttc tccttataga cagagttaga tttattagaa attttagaat attatatttt
     4261 attcgtaaac agatgcctgt caggcgtata tttcttctat gcgggttttc aggccgcaat
     4321 agcgtttctg ctccatggag ttatctgcca tagtataaaa tgcatccggc cggccgacca
     4381 ggcagacaca ggagcgggct cttgtcaccg ctgtatagat cagatttctg gtcatcagca
     4441 tacgtgggcc ggaatacata gggatgatca ccgccggata ttcacttccc tgggacttgt
     4501 ggatggtaat ggcataggca agttccagtt cttctaactg tttaaaactg tattctacca
     4561 ttttcccttc atcaaattcc acggtaagat tttccgcata ttcattgatc tcccggataa
     4621 tccccatatc accattaaat acaccggcac ctttatctgt gggaatgccg tatttgctgc
     4681 gaatctccca ttccatctgg taattatttt tgatctgcat cactttatcc ccggtgcggt
     4741 agatgatacc gccggactct ttctcttttt tagacggatc cggtgggttt aagtaatttt
     4801 gcagaatctg gtttagacgt tctactccca gagcaccttt tcgcataggc gtcattacct
     4861 ggatatcacc gggttgggcg tggacataac cgggaagttt ctgctggatc agtgtaatgg
     4921 ctgcgctgat cactgcatca ggattttccc tgcggataaa gagaaagtct ttactgcgct
     4981 ttgtcagatc tacccgttcc ccttcgttga tcttgtgggc atttacaata atatcgcttt
     5041 gggccgcctg acggaaaatg tgagtcagtt ttactacatt aaaacagccg gactcgataa
     5101 tgtcgcgaag cacatttcca ggtcctacac ttggaagctg gtccacatcg cctaccagta
     5161 tcagacgtgt tcctacgttt actgcgcgta acagggaatg catcagactg atatctacca
     5221 tggacatttc atcgatgata ataacatcgg catccagcgg attttcttca tttcggtcaa
     5281 aatgcatgcc accggaactg aaagagccat ttttaccgga aggggcatta ttcccagaca
     5341 tagaaccaga gtccgggata ccggatggca ccagttccag catgcggtga atggtacggg
     5401 cctcataacc ggtagtctca gtcattcgct tggctgcgcg gccggttggc gctgccagta
     5461 ggatctccat tccctcattt tcaaaatact ggataatagc attgatagtg gtagtctttc
     5521 ctgtaccggg accgccggtg atcactaaaa gtccgctgtt tactgcctca aaaactgctt
     5581 tttcctgcaa ttcatctaaa tggatttctt cttttttctg gactttcgcc agattggcac
     5641 ggatctctgt ttccggtata tcgccacgga tgtttaagtc atggagcata cgggccgtgt
     5701 tcagttccag ataatagtag tgggaagaat agaccagata ttccggcggt tcgctgcctg
     5761 ggactgtttt taccactact ttgcggtcga tttgcatatc catcagatgc ttttccatag
     5821 cagtcggttc tacttttaac aactctgccg cctgggaaaa taattctttc tgaggaagat
     5881 aggtatggcc gttggctgat gcctggagaa gaatgtaaag gagacctgcc ctgatacggt
     5941 agtcggagtc tgtgaagatg cctacttttt cagcgatctc gtctgccatt ttaaatccca
     6001 cacctggaat atcatctgcc agcttatagg gattttcttt gatgatactg tacagcttcg
     6061 ggccatattc ctggtagatc ttagccgcaa gatttaaagt aatgccataa ttttgcagaa
     6121 acatcatggc ttcccgcatg ccttttttct cttctacctg ttcagcaatg gccattgcca
     6181 ttttttcact gatgcctttc acttcggaaa ggcgctccgg ttcttcttcc atgacccgga
     6241 aagtgtcggc tttgaaatgg cggacaatcc gggctgccag tgctacaccg atacctttga
     6301 tggcaccgga acctaaatag cgctccatag actctgcgtc ctggggcgtt ttcatttcgt
     6361 aactttcgat ctgaagctgt tctccatata ccggatgctc gatcatgcgt ccggaggcct
     6421 ggatcatgtc tccttcagca gcataagaaa gcgtacctac catgataact tcatcgccat
     6481 cgctctggtc ggtaactgac agcacagtat agccattttc ctcgtttctg aattttattt
     6541 tttcaataaa tcctgttatg gttgccattt ttctttcctc tgtatctgtg tataagaaca
     6601 gttgctgctt ttatatagtc aaaaaggccg ccgcattgct gcggcagccc ggccagtcaa
     6661 aaaaattaat aatcctcttt atcgtttctt ccgccgccaa ttcccatgga tgcttccagg
     6721 gcggaggtca taccgtgtgc gaattcaatg aatttaccgg taccgatcgc tacggctgtc
     6781 agtggatctt cagcgatcac agtggtaata cctgtctttt cctcgatcag ggcatccaga
     6841 ccactgagaa gggagccacc gccggtcatt acgataccac ggtcaaaaat atcggctgcc
     6901 agttctggtg gagttctctc cagaacattg tgtacagcgt ctacaatctg gagagccggt
     6961 tctcttaatg cttccagagt ttcgtcggaa gtaactacaa tggtctttgg aagaccggtt
     7021 accaggttac gtcctcttac ttccatagtc tgtacttccg gtctgcggta tgcagcaccg
     7081 atattgatct tgatctcctc tgcagtacgt tcaccgataa gaagattgtg tttctttctc
     7141 atataacgga ctaatgcttc atcgaagtca tcacctgcga tcttgatgga agtgctgact
     7201 acaggtccgc ctaaggagat aactgcgata tctgcagtac caccaccgat atctacgatc
     7261 atgtttccgc atgctttacc gatatcaata cctgcgccga tggctgctgc tactggctct
     7321 tcgataatag caacttcacg ggcaccagcc tggtaagttg catcttctac tgctttcttc
     7381 tctacttctg tagcgccact tgggatacat acactgatac gtggcttacg cagtgttctc
     7441 ttgcctactg ccttgttgat aaagtatttc atcatttttt cggtaatggt atagtcggag
     7501 ataacgccct gacgaagagg acggactgct acgatattac ctggagtacg tccgatcatc
     7561 aggcgtgcct cttcaccgat cgccatgatc ttattggtat cacggtcaat ggcaactacg
     7621 gacggttctt ttaatacaac gccttttccc ttgatataca caaggatact ggcagttcct
     7681 aagtcaattc caatgtcatt ggacatcatt gaaaacatat aaatatttcc tcctaaaatg
     7741 tggggtcaca aaaagtttct tacaatagta gtttcttgta taactcaaaa aaagggcctg
     7801 cgctttaagc atgacaagta cgcctcagag cagactgaag tttcttctat tatatacaat
     7861 cttgaaaagt tttcaaaggt ttttttcact tatttatatt ctttattctt tttttatatt
     7921 atgtacattt tttggacaca tttattccgt attataatat cgttcaccga gagg
//
LOCUS       DBMDUA010000130         7954 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4054_length_7954_cov_2.565389, whole genome shotgun sequence.
ACCESSION   DBMDUA010000130 DBMDUA010000000
VERSION     DBMDUA010000130.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7954)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7954
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4054_length_7954_cov_2.565389"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="ACZPCL_09705"
     CDS             complement(<1..1291)
                     /locus_tag="ACZPCL_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162777.1"
                     /translation="MKKNITKKAAVFLLSTIVAAQSGLSVQAAALTEGSWEQTSSGWK
                     FLNRDRQPYTGWIHTASGWYYLNMEDGSLVTGWKNINGLDYYFTPANEGIEGQMKVGW
                     YQSPQGDWYFFDNTTDTHEEGSAVTGWNWIDGYCYYFARTEAGKGAKMAANTTTPDGY
                     KVNADGQWVNEDGVAQYRQSGGYRTKANSTTTVTSKSSGSGSGSSSGSDSDSGSTTPA
                     TPSTPDTPSTPDTPSTPDTPDVTEEYQYLLMNIPYADFYDSEVKGNDEDVDAVSSATL
                     NKTRTGSLVGGSYHEDPEGSEIRGVTFPVRVKKGTDLSSFKKVTDEDSVDITVTNRGQ
                     TNTTTYKGKDALFENEDFAYYELNEVPSYYKELTVSGAVLTFSEVKGTAKNVSGVSAT
                     VSASSSYGDYQLYMTGLPESLSNDSTTVYGVVIHTKEG"
     gene            1627..2715
                     /locus_tag="ACZPCL_09710"
     CDS             1627..2715
                     /locus_tag="ACZPCL_09710"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR1162778.1"
                     /translation="MKKRNLILLTTVCLALSACHPASAPSSGSKTSVSEASASKQEES
                     KDLAADESLSRELYAMDTVMNLTAYGPNASAALEASVSEINRLDSLLSISSEKGEIYR
                     INADKEGTVSEDVNALLSRSLELSQMTDGLFDCTIEPVMEAWGFTTQNFRIPSEEELE
                     ELLSHVDYKQVNLENSTVTIPEDVRLDLGGIAKGFTSSRVMDIFRENGVTSGIISLGG
                     NVQALGHKPDGNMWRVGIQDPHDLNSTFAVVEVADQAVITSGGYQRYFEENGQTYHHI
                     IDPRTGYPAENGLISVTIISADGTLADALSTSLFIMGPEEASSFWKKHRDTFDAIMMK
                     EDGTVMVTAGLADHCSVTTGGNVEVIQE"
     gene            2712..3110
                     /locus_tag="ACZPCL_09715"
     CDS             2712..3110
                     /locus_tag="ACZPCL_09715"
                     /inference="COORDINATES: protein motif:HMM:NF018685.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HIR1162779.1"
                     /translation="MKMNPSRKKTLILVIVLLLCAAFASFWTARQNRQNDAAVAVITY
                     QGKDIRTVDLSTVKETETFTLGEPGEQNTIQISPKGIGVIEADCRDQICVKQGIHSHG
                     PEPIVCLPHKLSIRFISSNTDNSLDAVTGR"
     gene            3147..3488
                     /locus_tag="ACZPCL_09720"
                     /pseudo
     CDS             3147..3488
                     /locus_tag="ACZPCL_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012472.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            3528..3791
                     /locus_tag="ACZPCL_09725"
     CDS             3528..3791
                     /locus_tag="ACZPCL_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024581.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1162780.1"
                     /translation="MPCTTCNYCAKVCPMDIGISGSFTAMNYLTLYKDKGMAAHQEQW
                     LVGGHGRKAADQCIKCGKCESVCPQHIAIRKNLEVVAENLLAK"
     gene            complement(3945..4424)
                     /locus_tag="ACZPCL_09730"
     CDS             complement(3945..4424)
                     /locus_tag="ACZPCL_09730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1162781.1"
                     /translation="MRYLQSVNDDGSGVSLTDRKVCLLSEKGTSTQKACDGGTITADQ
                     LESLDNIVQIHRRNLDLKHIKDLCKDIEERFPKVSAYANLWNGDIVARGVDKAVAVKW
                     LKEHFPQIEKIRCIGDSVNDLGMVRAYQGAVPDVADPEIKKEATQIVKSVAEFLENF"
     gene            complement(4431..4694)
                     /locus_tag="ACZPCL_09735"
     CDS             complement(4431..4694)
                     /locus_tag="ACZPCL_09735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1162782.1"
                     /translation="MKCAVSDFDRTLYVERMISEEDKNAVLNWQKSGNLFVIATGRNL
                     GTIKPIFESYGFSPDLWILNNGAMIADRDGKILFTKLIPRETA"
     gene            complement(4691..5875)
                     /locus_tag="ACZPCL_09740"
     CDS             complement(4691..5875)
                     /locus_tag="ACZPCL_09740"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046349 - amino sugar biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR1162783.1"
                     /translation="MVLYSNYIVLEHGIFEGFLELENGKIKGLYEKWQGEYKDYSDKI
                     IFPGFIDIHVHGWATGSFWFEKTPQSLREMCRTLPFAGVTSYLGTTGADPIEEIKTCI
                     RAADQVSEEDPEGAQLLGVHLEGPFINPVYKGMQKEECCLLPDVTVMEDLYNTFKNKK
                     LCRHMTIAPERPGADAVLRFCQEHQIQTAVGHSAATFEEIKKMRAYGLGGFTHTFSGM
                     KGFHHRELGTAGAALYFDDMICEFAKQTGMTVSHEAFELAYRIKGSSRIVLTTDCCGL
                     AQTQSCFDHYVRKIRFVKDGDQVCLEHYDGKKEWIDPRDYQAVKQVEMSYAQSVKNMA
                     DHTKVGLYDIMLMTSFNPAHYIHADDCKGSIRPGMDADLTIMDQNLDLICVYCRGKEI
                     RE"
     gene            complement(5892..7214)
                     /locus_tag="ACZPCL_09745"
     CDS             complement(5892..7214)
                     /locus_tag="ACZPCL_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 4 glycosyl hydrolase"
                     /protein_id="HIR1162784.1"
                     /translation="MRKPHIITIAGAGSARVPALVGTLVQYKERFPVSKMIFYDIDGE
                     RMGKMEAYDCLVLKCFYPECDVVFTTDEDEAYSHTDFIFCQMRVGKTEMRSLDEKIPL
                     KYGLIGQETCGPGGFAYGMRSLGAMKQMVEKVRSYSKDTWILNYTNPAAIVALGLDQM
                     FPDDKRILNLCDQPFSMMKSFAKILGVPQEILRAKYFGLNHFGWFTDLYGTDGKNYFD
                     QLRTYLRDHEFKPYNAEQRSKSWLETYVRVNKYMQYLDEYVPTTYLQYYMFPDEIVKE
                     SDPNYTRADEAKDSREKDVFETCAKAVGRDTMDPSEMLTNSVFGNLMVEVAESIAYDL
                     NNEFVVLVRNEGIIPNFESRAIIEVAGTIGKDGAKGYPYGDIDPYYKGLMEGQYAYEQ
                     LTVEAFREKSYTKALKALILNRSVIDPSKAGAVLDDLMEANKDYWYLT"
     gene            complement(7246..7710)
                     /locus_tag="ACZPCL_09750"
     CDS             complement(7246..7710)
                     /locus_tag="ACZPCL_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1162785.1"
                     /translation="MGLFGKKKVDFVSPAGGRLMNITEVADPAFSSKMLGDGFAVDLT
                     DGEIGAPFDGEVTAAFPTGHAFGLKRKDGLECLIHVGMDTVQLNGKGFDVKVKQGQMV
                     KKGDTLVVVDLDEVRAAGKSLVTPVIFTEGTPVKMLKKDTQISRGEEDILKF"
     gene            complement(7775..>7954)
                     /locus_tag="ACZPCL_09755"
     CDS             complement(7775..>7954)
                     /locus_tag="ACZPCL_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162786.1"
                     /translation="LEGKNYLQVLSDDEVLSSMFTKEELEKMIAPSSYTGICSVLARE
                     LADKAEAKAKMMTEK"
BASE COUNT         2062 a         1982 c         1558 g         2352 t
ORIGIN      
        1 taccttcttt tgtatggatc actacgccat atacagttgt gctgtcatta gacagagact
       61 ccggaagtcc ggtcatatac agctgataat cgccataaga gctgctggct gatacagttg
      121 cacttacacc gcttacattt ttagccgtac cctttacttc gctgaatgtc agtacagcac
      181 cggaaactgt cagttctttg taataggaag gaacctcatt taattcatag taagcaaaat
      241 cctcgttttc aaataaagca tcttttcctt tgtaagttgt agtgtttgtc tgtccacggt
      301 ttgtaacagt aatatctaca gagtcttcat ccgttacttt cttaaaactt gaaagatcgg
      361 ttcctttctt tactcttaca gggaatgtaa cgccgcgaat ctcgcttcct tctgggtctt
      421 catgatagga accaccaacc aggcttcctg ttcttgtttt atttaaagtt gcagaagaaa
      481 cggcatctac atcctcatcg tttcctttta cttcactgtc ataaaagtct gcataaggaa
      541 tgttcatcaa cagatactga tattcttctg ttacatctgg agtatccggg gttgatgggg
      601 tgtctggagt agatggggta tccggagtgg acggtgtagc aggcgtagta gaaccggagt
      661 cactgtcgga acctgaactg gagccggaac ctgatccgga actcttggaa gtaaccgtgg
      721 ttgtactgtt tgctttcgtg cggtatccgc cagactgacg gtactgtgca acgccgtctt
      781 catttaccca ctgaccatct gcatttactt tgtagccgtc tggtgtagta gtgttggctg
      841 ccatttttgc tccttttcca gcttctgtac gggcaaaata atagcaatag ccgtcgatcc
      901 aattccatcc tgtgacagcg cttccttctt catgagtatc tgtggtatta tcgaagaaat
      961 accagtctcc ctgtggtgac tgataccagc ctaccttcat ctggccttcg atcccctcat
     1021 ttgccggagt aaaataataa tccagaccat tgatattctt ccagcctgtt accagacttc
     1081 cgtcttccat attcagataa taccagcctg atgctgtgtg gatccagcct gtatatggct
     1141 gacggtcacg atttaaaaac ttccatccgg aactggtctg ttcccagctt ccttctgtta
     1201 aagctgcagc ctgtacagac agtcctgact gagctgctac gatggtagac agcagaaata
     1261 cggctgcttt tttcgtaatg ttctttttca taggttcctc cattccccgg cataaaaaca
     1321 tgcctgtttt agttagctat cactaacttt cttacaaaaa aataaacttt ttattaatac
     1381 aagtcaatct ttcgtccccg tttcagcccc tctttattgt tatttttttc tcattagaaa
     1441 gttgttgaaa aaaaaagtca gattttcccg tttttaaaga ctccaaaagt atctgtccct
     1501 gcaaagtaat gaattgacaa ccattttgct caatgttaag atgtgacaca gaaagattag
     1561 cattttctaa cctttgttaa atctatgatt tagatgtaat taacatactg atatgaggtg
     1621 caacctgtga aaaaacgaaa tctgattctg cttacaactg tctgcctggc tttatcggcc
     1681 tgccatcctg cttctgctcc ctcttccggc tccaaaactt ctgtttctga agcttccgct
     1741 tcaaaacagg aggaaagcaa agatctggca gcagacgaaa gcctttcccg cgagctttat
     1801 gctatggata ccgttatgaa cctgactgcc tatggtccca acgcctctgc tgccttagag
     1861 gcttctgtct ctgagatcaa ccgcctggac agtctgttat ccatttccag tgaaaaggga
     1921 gagatctacc ggatcaatgc agataaagaa ggaactgttt cagaagatgt aaatgctctt
     1981 cttagtcgtt ccctggaact ttcccagatg acagacggat tgtttgactg taccattgaa
     2041 ccggttatgg aagcctgggg ctttaccacc cagaatttcc gtatcccttc tgaagaagaa
     2101 ctggaggaac ttttatccca cgtggattat aaacaggtaa atctggaaaa ttctacggta
     2161 accattcccg aagatgtccg cctggatctt ggaggtattg ccaaaggttt tacttcttcc
     2221 cgtgtaatgg atattttccg tgaaaatggt gttaccagcg gcattatctc ccttggtgga
     2281 aacgtacaag ctttaggaca caaaccggac ggaaatatgt ggcgcgtagg cattcaggat
     2341 ccacatgatc tgaactctac ctttgctgta gtggaagtag ctgaccaggc agtgatcacc
     2401 tcaggcggct atcagcgcta ttttgaggaa aatggacaga cctaccacca tatcattgac
     2461 ccacggaccg gatatcctgc tgaaaacgga ctgatctccg ttaccattat cagcgctgac
     2521 ggaactctgg cagatgccct ttccacctct ctttttatca tggggccgga agaggcttcc
     2581 agtttctgga aaaaacacag agacaccttt gatgcgatca tgatgaaaga ggatggtact
     2641 gttatggtaa ccgccggtct tgcagaccac tgcagcgtta ctactggcgg aaatgttgag
     2701 gtgatacagg aatgaaaatg aacccatccc gtaaaaagac tcttattctg gtcattgtcc
     2761 ttttgctttg tgccgctttt gccagttttt ggacagcccg gcaaaaccgg caaaacgacg
     2821 ctgcagttgc agtcatcacc tatcagggta aagacatccg caccgtagat ttaagtacgg
     2881 taaaggaaac ggaaaccttt acccttggag aacctggaga acagaatacc atccagatct
     2941 ccccaaaagg gatcggtgtg attgaagcag actgcagaga ccagatctgc gtcaaacagg
     3001 ggatccactc ccatgggccg gaacccatcg tttgcctgcc tcataaactg agcatccgtt
     3061 ttatctcttc taatacagat aattccctgg atgcagtgac cggacggtaa aaatatcata
     3121 aagaaaccgg aaacttaaca gaacatcctg aaatggaatt tgtacagctg cagatcaact
     3181 acgctgactg ggaaaatccg gctgttcagt ccagaagatg ttatgaagta gcaagaaaac
     3241 acggaaaacc tgtgatcatc atggaacctg taaaaggcgg aatgttggca aatcctccgg
     3301 agtctgttgc aaatatctta aaggctgctg aacctgactc ttccgtagca tcctgggctg
     3361 tacgttttgc tgccaacctg gaaggtgtga tcactgtcct ttccggtatg agcaatgtag
     3421 aacagatggc tgacaacctt tcttatatga agagtttcac cggcttaact gacgctcaga
     3481 aggataccct taaaaaggct caggaagaat tagcccgcat ccctctgatc ccatgtacta
     3541 cctgtaacta ctgcgcaaaa gtctgtccaa tggatatcgg tatctccggc tccttcactg
     3601 ctatgaacta cctgaccctg tacaaagata agggaatggc agctcatcag gaacagtggc
     3661 tggtaggcgg tcatggacgc aaagctgctg accagtgtat taagtgtggt aaatgtgagt
     3721 ctgtatgccc gcagcacatt gccatcagaa agaacctgga agtcgttgca gaaaatctgt
     3781 tagcgaaata aagatctgtt tccaacggag ctgtcacctg gcggtgacgt attcctaata
     3841 taacaaacaa catagcaaaa agccgtttcc ctggttttac tttcaaaccc ggaaacggct
     3901 ttttagttta aactttgatc tgaaattctg atcaggatct ttatttaaaa attctcaaga
     3961 aactctgcta cactctttac gatctgcgtt gcctcttttt tgatttcggg atctgctaca
     4021 tccggaactg ctccctggta ggcccgtacc attcccagat cattgacact gtctccaata
     4081 caacggatct tttcgatctg cggaaaatgt tctttcagcc attttacagc aactgcctta
     4141 tctacgcctc tggctacaat atcaccattc cacagatttg cataggcaga aactttcgga
     4201 aaacgctctt caatgtcttt acaaagatct tttatatgct tcaggtccag atttcttctg
     4261 tggatctgta caatattatc cagactttcc aactgatcgg ctgtgatcgt tccgccatca
     4321 caagctttct gggtactggt ccctttttca gaaaggaggc atactttccg gtctgtaagg
     4381 gatacaccgc ttccatcatc atttacactt tgcagatagc gcaatactgc ttatgccgtt
     4441 tcccttggga tcagctttgt aaacagaatc tttccatccc tgtctgcgat catggcacca
     4501 ttatttaaga tccaaagatc cggcgaaaag ccataagact caaaaatagg ctttatggtc
     4561 cctaaattcc ttcctgtggc gatcacaaag agatttccgg acttctgcca gtttaaaacc
     4621 gcattcttat cctcttcact gatcatccgt tccacatata aggttctgtc aaaatcactg
     4681 actgcacatt tcattcccgt atttcctttc ctcgacagta aacgcagatc agatccaggt
     4741 tctgatccat gatagtcaga tctgcgtcca ttccaggccg gatgctgcct ttgcagtcat
     4801 ctgcatggat ataatgagcc ggattgaagc tggtcatcaa catgatatcg taaagcccca
     4861 cttttgtgtg atctgccata tttttcaccg actgggcata actcatctcc acctgtttta
     4921 ctgcctgata atcccttgga tcgatccatt ctttctttcc gtcatagtgc tctaagcaca
     4981 cctgatcccc gtcttttaca aaccggatct ttcttacgta atgatcaaaa cagctttggg
     5041 tctgggcaag accacagcag tctgttgtca gcacaatacg gctgcttccc ttgatcctgt
     5101 aagccagttc aaaagcttca tgggacaccg tcattcctgt ctgctttgca aactcacaga
     5161 tcatatcatc aaaatacaaa gctgctcctg cagttccaag ttccctgtgg tgaaatccct
     5221 tcattccgct gaatgtatgg gtaaatccac caagaccata ggcccgcatt ttcttgatct
     5281 cttcaaaagt agctgcactg tgtcccacag ctgtctggat ctggtgttcc tggcaaaatc
     5341 taagaacagc atctgctccc ggccgttccg gcgctatggt catatggcgg cacagttttt
     5401 tatttttaaa ggtattataa aggtcctcca tcactgtcac atccggaagc agacaacatt
     5461 cttctttctg cattcccttg tatacaggat tgataaatgg tccttccaga tgaaccccta
     5521 aaagctgcgc tccctcagga tcttcttcgc tgacctgatc agcagcccgg atacaggtct
     5581 tgatctcctc aatgggatct gcccctgtgg ttcccagata agaagtaaca cctgcaaaag
     5641 gcagggtacg acacatctcc cgaagagact ggggtgtttt ctcaaaccaa aaagaaccgg
     5701 tagcccagcc gtgaacatgg atatcaataa atccggggaa aatgatcttg tcggaataat
     5761 ctttatattc tccctgccat ttttcataaa gcccttttat cttcccattt tccagttcca
     5821 ggaacccttc aaagatcccg tgttccagta caatatagtt tgaataaaga accatgtcac
     5881 tccattctgt atcaggtaag ataccagtaa tctttgttgg cttccatcag gtcatccaaa
     5941 actgctcctg ccttggaagg atcgatcaca gatcgattta agatcagggc tttcagggct
     6001 ttcgtataac tcttttcccg gaatgcttct actgtcagct gttcataagc atactgtcct
     6061 tccatcagtc ccttataata aggatcgatg tcaccataag gatagccctt tgcaccatct
     6121 tttccaatag ttcccgctac ttctatgatc gccctgcttt caaagttggg gatgatccct
     6181 tcattgcgca ccaggactac aaattcattg ttcaggtcat aggcaatgga ctctgccact
     6241 tccaccatca gatttccaaa cacactgttt gtaagcattt ctgacggatc catggtatct
     6301 ctgcctactg cctttgcgca ggtttcaaat acatcctttt ccctggagtc ttttgcttca
     6361 tctgcacggg tatagttagg atcactttct tttacgatct catccgggaa catgtaatac
     6421 tgaagatagg tagttggaac gtactcatcc agatactgca tatatttatt tacacggaca
     6481 taggtctcca gccaggattt ggatctctgc tctgcattat aaggcttaaa ttcatgatca
     6541 cgcagatagg tccttaactg gtcaaaataa ttctttccgt ctgttccata caggtctgta
     6601 aaccatccaa aatggtttaa accaaaatat ttagccctta agatctcctg aggcactccc
     6661 aggatctttg caaaactttt catcatggaa aatggctgat cgcagagatt taagatccgc
     6721 ttatcatccg ggaacatctg gtccagtccc aaggccacga tagctgccgg attggtatag
     6781 tttaagatcc aggtatcctt ggaataagaa cgtacttttt ccaccatctg tttcatggct
     6841 cccaaagagc gcattccata ggcaaatccg cccggaccgc aggtctcctg tccgatcaga
     6901 ccgtacttaa gaggaatttt ctcatccagg gagcgcattt ctgtttttcc tacacgcatc
     6961 tggcagaaaa taaaatctgt atggctgtag gcttcatcct catccgttgt aaatacaaca
     7021 tcacactccg ggtaaaaaca ttttaatacc aggcagtcat aggcctccat ctttcccata
     7081 cgttccccgt caatatcata aaagatcatt ttagataccg gaaaacgttc tttatactgc
     7141 accagtgttc ccaccaatgc aggaacgcgg gcgcttcctg ctcccgcgat agtaataata
     7201 tgtggtttac gcatacttct cctcctgctt ataggttctc tttgatcaga actttaatat
     7261 atcttcttct ccccttgaga tctgtgtatc cttctttaac attttcacag gtgttccctc
     7321 tgtgaagata accggtgtta ccagtgattt tccggcagca cgtacctcat ccagatcaac
     7381 aactaccagg gtatctccct tctttaccat ctggccctgt tttaccttaa cgtcaaagcc
     7441 tttgccattt aactggactg tgtccattcc tacatggatc aggcactcta atccgtcttt
     7501 tctctttaag ccaaaagcat gtcctgtagg aaacgcagct gttacttccc cgtcaaaagg
     7561 cgcaccgatc tctccgtctg tcagatccac agcaaatcca tcacccagca ttttagaaga
     7621 aaaggcagga tctgccactt ctgtaatatt catcagtctg cctcctgccg gggacacaaa
     7681 atctactttc ttttttccaa ataatcccat tttttctcct tctatcctgc cggtcagatc
     7741 cggcattcca tctttattcc tttatcttat ttctttattt ctctgtcatc atttttgcct
     7801 tggcttccgc tttatctgct aattccctgg caagaacgct gcaaattcct gtataactgg
     7861 aaggagcgat cattttttcc agctcttcct ttgtaaacat agaagataaa acttcatcat
     7921 ctgacaacac ctgaaggtaa ttctttcctt ccag
//
LOCUS       DBMDUA010000131         7947 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4059_length_7947_cov_4.758858, whole genome shotgun sequence.
ACCESSION   DBMDUA010000131 DBMDUA010000000
VERSION     DBMDUA010000131.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7947)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4059_length_7947_cov_4.758858"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="ACZPCL_09760"
     CDS             <1..186
                     /locus_tag="ACZPCL_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1162787.1"
                     /translation="KENPTIEDYNEFLESRCFPRTRDKMKLVLRDLGIPFYDPYLIIQ
                     KTEGRMAEDDFWIRIER"
     gene            198..1007
                     /locus_tag="ACZPCL_09765"
     CDS             198..1007
                     /locus_tag="ACZPCL_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162788.1"
                     /translation="MIELFEQNERMQDRQSSKGNQLKWENAGIWYKADYTGYEGLAEY
                     MISHLLIKSTLKPEEFVRYDLEEIKYKRQVYQGVKSRTFLSEDWQIITLERLFQKMYG
                     NSLNQMLWKMDDAEQRLRFLEEQVERMTGLRGFGIYLNKLLTIDAIFLNEDRHTHNIA
                     VLMNGAGMFKYCPIFDNGGGLLSDTILDYPLGEDTFDLMETVWAKTVSTDFDEQLDVS
                     EHLYGCNLKFFFTKRDVDQLLEQAKGYSDEVRERVQTILHRQIDKYAYLKM"
     gene            complement(1073..2557)
                     /locus_tag="ACZPCL_09770"
     CDS             complement(1073..2557)
                     /locus_tag="ACZPCL_09770"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF012589.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR1162789.1"
                     /translation="MLVATFDIGTTAVKAVAVRDDGEPFFTGSQVIHTIEKNGFKEQN
                     PEEWYSAFCALSHALTDKISAKDISALIFSGQMQDVIPVGSDLRSLGNAILYSDGRAG
                     AQAEAIENAVGSDTITAITGNHFDGSMPLAKILWLKENQPEIYEKAAAFLISSKDYVV
                     ARLTGNFIGDMTACATAGAMDLEHKTWSEHLINDAGLDINKFPKLLHSHELAGVIAEK
                     GAADTGYLPGTKVYAGTGDAGATTLASGILAPGEYNVNLGTSSWVAAVSKGRMDSEGG
                     GFNLAAMQKDLYINVVPFLNGGNVHRFIARILSRELDHKPDFAYISHLLKNHVPGSHG
                     VFFLPYLTGERFPIMDSHIRASFLGLSQDTSATDMAGSVLEGVAFSIRHGLELFNAPA
                     VKLSIIGGGARELAWCQILSNVMGLPVHVYKDPDLLPALAIASSVFLAENIIPDYQSF
                     IHVLEEKGGCTVLEPDPDAVKAYKPVYEKYKTIYPMIREFYHEV"
     gene            complement(2574..3503)
                     /locus_tag="ACZPCL_09775"
     CDS             complement(2574..3503)
                     /locus_tag="ACZPCL_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1162790.1"
                     /translation="MKAFKHYGTVAALAVIILIFSILRPASFCTVKNFLNISRQAAPL
                     AMVALGATFVMTINEFDLSIGNIVSLGGVIAALLSIKQVPVALCFLIAAVICLLVGCV
                     NGIVVARFQILSFITTLGMGTVLDGVIYGLTGGTTVFNGIPKAFTIPGILKIAGIPFI
                     TLVAVVLYLAFYVFMGHTAPGRKLYAIGGSKEAAEVAGIPIFRYRVLAFSLCGAIAGL
                     AGTLVASRVGSANTTAGQGYFLQSYAAVFIGCTVSKSGVPCVVGTLIGAAVLAVLANG
                     LTILQMPSFMQNIITGGIIILAVVIQKIGAGKN"
     gene            complement(3490..5034)
                     /locus_tag="ACZPCL_09780"
     CDS             complement(3490..5034)
                     /locus_tag="ACZPCL_09780"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1162791.1"
                     /translation="MLLEAKKLDKSFNGVYALKGLDLSLKEGEIHGLVGENGAGKSTF
                     IKILGGVYTKNGGELSWNGAPFPADFGPVESRELGINIIFQDHVLIPAFSALENIWLG
                     RPYPRKGGLIQWKEMEKAAQKKGEELGIHLDLKKSIFQMTPAQKKCVEILRAMMSDCK
                     LMILDEPTASLSDKETNLLFSIIRNLKAKGTAVLYVTHRLEEIMALTDRVTVLRNGEL
                     VSTCDTASTSQKELVRLMSGNGEADSKHMMPDSTCCSRSAATQSFPALTISHLSSADH
                     IVKDASITVHTGRITGMFGLCGSGRTEFLECIYGIRRISGGTIEISGEKVKKPTPVNS
                     IKKGIAFICEDRRRKAMIPSFTVEENMNLSSIDHYSKKGLFSTTAAVSAAKGMINALK
                     IRCTGTSQPACELSGGNQQKVVFAKALLTSPSIWLCDEPTQAVDVATRQQIHSLLHHQ
                     AAENKAVLYVSSDLTELLEVADEIAVMASGKIRKIFPNKNLKPADILACCYEAEREET
                     HENESI"
     gene            complement(5127..6185)
                     /locus_tag="ACZPCL_09785"
     CDS             complement(5127..6185)
                     /locus_tag="ACZPCL_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR1162792.1"
                     /translation="MKKMRNFLTLAAAAALTGILLTGCSTEADETTAPKAETAAQTEA
                     ASTTEKASEDAASGEADTKTADSTALAGKKISVMTPYLTSVTTNQMAGFIKNNLEAEG
                     AEVFVIDTANDFAELASRIEDVVSSGTDGIVLVSADPNQVANQLTEAFDADIPVFGCD
                     SGFIDGMQVNATSDNYQMGELIVRYLFDDLMGGKGTVIALTHRPHPGVVKRCEAFDDI
                     IKEYPDIQLITEQHVPAEQPINDAEEITANLLTANPDKDSVTAIFAAWDEPAIGAAKA
                     LAEAGRDEVIVTGVDGNEQAVQMIKDGSCLKATVKQNFEGMADIVCQQMEKYYAGETI
                     EKGEMYAPAELITQENAQ"
     gene            6855..7469
                     /locus_tag="ACZPCL_09790"
     CDS             6855..7469
                     /locus_tag="ACZPCL_09790"
                     /inference="COORDINATES: protein motif:HMM:NF017927.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIR1162793.1"
                     /translation="MTRAELKRNAREKLGGQLFGPNWVNAVLVMAIFYILTGAVNGIA
                     GFVTLIMLVIGGPLSYGVAKLFLQQCDDGQKMNPTEVFKGFSEDFGGSFILYLLRYLF
                     IALWSILLIIPGIVKMYSYSMAFFIKADHPSYDWRECLDASSQLTYGHKWELFILDLS
                     FIGWQIVGSLCLGIGTFWVNAYREATIAEYYRYYESNQVIDRDF"
     gene            complement(7552..7797)
                     /locus_tag="ACZPCL_09795"
     CDS             complement(7552..7797)
                     /locus_tag="ACZPCL_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HIR1162794.1"
                     /translation="MNYTTHGVCSRAISFEVKDNKVTNVHFVGGCSGNTQGVAALVEG
                     MDIDEAIKRMKGIKCGPRPTSCPDQLATALEQYKAQQ"
BASE COUNT         2149 a         1753 c         1832 g         2213 t
ORIGIN      
        1 aaggaaaatc caaccattga ggattataat gaatttttgg aatcaagatg cttccctaga
       61 acgagagata agatgaaact ggtgttaaga gatcttggaa tcccatttta tgatccatac
      121 ctgatcattc agaaaacaga gggaagaatg gcagaggacg atttttggat caggatcgag
      181 cgataggagg aatggcgatg attgagttat ttgaacaaaa tgaacgaatg caggaccgtc
      241 agtcctcaaa aggaaatcag ttgaaatggg aaaatgcagg aatttggtat aaagcggatt
      301 atactggata tgaagggttg gcagagtata tgatctctca tttactgata aaatctacat
      361 taaaaccaga agagtttgtg cgatatgatc tggaagaaat taagtataag cgtcaggtct
      421 atcagggagt aaaaagccgt acttttttat cagaggactg gcagatcata actttggagc
      481 ggttgtttca aaaaatgtat ggaaatagtt tgaaccaaat gctttggaag atggatgatg
      541 cagagcagag gttgcggttt ctggaagaac aggtagagcg gatgactggt ttaagaggat
      601 ttggcattta tctgaataaa ttacttacaa tcgatgcaat tttcctaaat gaggacagac
      661 atacccataa tattgctgtt ttaatgaatg gagcaggaat gtttaaatat tgcccgatct
      721 ttgataatgg aggcgggctg ctgtcggata caatactgga ttatccatta ggagaagata
      781 cttttgatct tatggaaact gtttgggcaa aaacggtgag tactgatttt gatgagcagt
      841 tagatgtttc ggaacacttg tatggatgca atttaaagtt tttctttaca aagcgggatg
      901 tggatcagtt gttggaacag gcaaagggat attcagatga ggtgcgggaa cgtgtacaga
      961 cgatcctgca tcgacagatt gataaatacg catatttaaa aatgtagtaa atcgcaactg
     1021 actaaaaatc tcccggcata tcttctaaat taagatatac cgggaggttt gattacacct
     1081 catgataaaa ctcacgtatc ataggataaa tggtcttata tttctcataa acaggtttat
     1141 aggcttttac agcatccggg tccggttcca gtacggtgca gccgcctttt tcctctaata
     1201 catggataaa ggactggtaa tccgggataa tgttttcggc caggaaaacg cttgatgcaa
     1261 tggcaagtgc gggcagtagg tccgggtcct tgtagacgtg gacgggaagg cccattacat
     1321 tgcttaagat ctggcaccag gcaagttctc tggctccgcc gccgatgatg gaaagcttta
     1381 ccgccggagc gttaaacagc tctaagccgt gtcggatgga aaatgccacc ccctctaata
     1441 cgcttccagc catgtctgtg gcagaggtat cctggcttaa gccaaggaaa gaagcgcgga
     1501 tatggctgtc cataataggg aaacgttcac cggtcagata gggcaggaaa aagacaccat
     1561 ggcttccggg aacatggttt tttaaaaggt gggagatata ggcgaaatcc ggtttatggt
     1621 ccagctcgcg ggataagatc cgggcaataa agcggtgtac atttccgccg tttaaaaatg
     1681 gtactacatt aatatataag tctttttgca tggcagccag gttaaaaccg ccgccttcac
     1741 tgtccatacg gccttttgat acagctgcca cccagctgga agtacccagg tttacattgt
     1801 attcgccagg ggctaagata ccactggcaa gagtggtagc cccggcatct ccggtgcctg
     1861 cgtagacttt tgttccgggc agatagccgg tatcagcagc tcccttttct gctattacgc
     1921 ctgcaagttc atgggaatgg agcagttttg gaaatttgtt tatatccagt ccggcatcgt
     1981 ttataaggtg ctcagaccat gttttatgtt ccagatccat agctcccgca gtagcgcagg
     2041 ccgtcatatc accaatgaaa ttcccggtca gtcttgccac tacatagtct ttggaactga
     2101 tgagaaaagc agctgctttt tcatagatct ccggctgatt ttcctttaac cacaagatct
     2161 ttgccagagg catggaaccg tcaaaatggt ttcctgtaat agcggtaata gtatcgctgc
     2221 caactgcatt ttcaatggct tctgcctgtg cgccagcccg tccgtcagag tagagaatgg
     2281 catttcccaa agatcttaag tcacttccaa ctggtatcac atcctgcatc tgaccggaaa
     2341 aaataagagc ggaaatgtct ttggccgata ttttatctgt aagagcatgg gataaggcgc
     2401 agaaagcact gtaccattct tccgggttct gttccttaaa accatttttt tctatagtat
     2461 ggatcacctg gcttcctgta aaaaacggtt ctccatcgtc acgtacagct accgccttta
     2521 ctgcggtagt tccaatgtca aaggttgcaa ctaacataat tttaccccct aagtcaattc
     2581 tttccggccc ctattttctg gatcacaact gccagtataa tgataccgcc ggtaatgata
     2641 ttctgcataa agcttggcat ctgcaaaatg gtcagaccgt tggctaaaac cgccagaact
     2701 gctgcgccga tcagcgttcc cacgacacag ggaacaccgc ttttagatac agtgcagccg
     2761 ataaatacgg ctgcataaga ctggagaaaa tagccttgtc ctgcagtagt gttggcagag
     2821 ccaactctgg aagccaccag agtacctgcg agacctgcaa tcgcgccaca cagggaaaat
     2881 gccagtaccc ggtagcggaa aataggaatt cctgctactt cagccgcttc cttgctgccg
     2941 ccgatggcat acagcttccg gcccggagca gtatgtccca tgaaaacata aaatgccagg
     3001 tataaaacaa ctgccaccaa agtaataaag ggaatacctg cgatctttaa aataccggga
     3061 atggtaaatg cctttggaat accattaaat acggtagtac cgccggtgag cccgtagatc
     3121 acaccgtcta atacagttcc cataccaaga gtagtaataa aagataaaat ctggaagcgg
     3181 gcaactacaa taccatttac gcagccaacc aaaaggcaga tcacagcagc aatcaggaaa
     3241 caaagagcca caggaacctg tttaatggat aacagggcag cgatcacgcc cccaaggctt
     3301 acaatatttc caatggataa gtcaaattca ttgatggtca ttacaaaagt agctcctaaa
     3361 gccaccatag ccaatggtgc tgcctgtctg ctgatgttaa gaaagttttt aactgtgcag
     3421 aaagaggccg ggcggagaat agaaaaaata aggatgatca ccgccagtgc agctacggtt
     3481 ccataatgtt taaatgcttt cattttcatg tgtttcctct ctttctgctt cataacagca
     3541 ggccagtatg tccgctggct ttaaattctt atttggaaag atcttacgta ttttcccgga
     3601 tgccatgacc gcgatctcat cagctacctc caaaagttct gtaaggtccg atgaaacgta
     3661 gagaacagct ttgttttctg ccgcctgatg gtgcagcaga ctgtggatct gctgtctggt
     3721 ggcaacatct acggcctgtg taggttcatc gcacaaccat atggaggggg atgtaagtaa
     3781 tgcttttgca aagaccacct tctgctggtt gccaccggat aattcacagg ccggctggga
     3841 agtgccggtg cagcggatct ttagggcgtt tatcatacct tttgcagcag aaacagcagc
     3901 tgttgttgaa aacagcccct ttttggaata atggtcaatg gaagaaaggt tcatattttc
     3961 ttccactgta aaagacggga tcatggcttt gcgccgcctg tcttcgcaga taaaggcgat
     4021 gccctttttt atggaattaa caggagtggg ctttttcacc ttttccccac tgatctcaat
     4081 ggttccgccg gatatgcgcc ggatgccgta gatacattcc agaaattccg tccgtccgct
     4141 gccgcaaaga ccgaacatgc cggtgatgcg tccggtgtgg actgtgatac tggcgtcttt
     4201 tacaatgtgg tcggcagagg aaaggtggga gatggtgagt gcagggaatg attgtgtggc
     4261 tgccgaccgg gaacaacatg tactgtcagg catcatgtgt ttactatccg cttccccatt
     4321 cccgctcatc agccggacca gctccttctg gctggtagag gcagtatcgc aggtacttac
     4381 cagttctccg tttctcagca ctgttacccg gtccgtcagc gccatgatct cttccagcct
     4441 gtgggttaca taaaggacag cagttccttt tgctttcagg ttccttataa tagagaatag
     4501 taaattcgtt tccttatcag aaagagaagc agtaggctca tctaagatca ttaacttaca
     4561 gtcgctcatc atggcccgca ggatctctac gcatttcttc tgtgccggag tcatctggaa
     4621 aatggatttc ttcaggtcta aatggatccc aagttcttcc ccttttttct gagccgcttt
     4681 ttccatttcc ttccactgga tcagaccgcc ttttcgggga taaggccgtc caagccagat
     4741 attttccagg gcggaaaatg caggtatcag cacatgatcc tggaaaatga tattgatgcc
     4801 cagttcccgg ctctctaccg ggccgaaatc agcaggaaaa ggagctccgt tccaggacag
     4861 ctcccctccg ttctttgtat acacgccgcc taagatctta atgaacgtag actttccagc
     4921 gccattttca cctaccaggc cgtggatctc cccttctttt aaagagagat ccagaccctt
     4981 aagggcatat acaccattaa atgatttgtc cagtttcttt gcttctaaca gcattcttgc
     5041 cacctgccat tttccatgat ctgatcctaa tctgtatcag cctaaattcc cgggccccgc
     5101 ctggagcccc tgggaaattc gttctgttac tgtgcgtttt cctgagtaat aagctctgca
     5161 ggagcgtaca tttcaccttt ttcaatggtc tctcctgcat aatatttttc catctgctgg
     5221 cagacaatgt cagccatgcc ttcaaaattc tgctttacag ttgcttttaa gcaggaaccg
     5281 tctttgatca tctgtactgc ctgctcattt ccgtcaacac cggtaacaat aacttcgtca
     5341 cgtcctgctt ctgccaatgc ctttgcggcg ccgatggccg gttcatccca tgctgcgaaa
     5401 atagcagtta cagagtcctt gtcagggttt gcggtcagca ggttagccgt gatctcttct
     5461 gcatcattaa tcggctgttc agccggtaca tgctgttctg tgatcagctg aatgtcagga
     5521 tattctttaa taatgtcatc aaatgcttca caacgtttta caacacctgg atgtggtctg
     5581 tgggtgagtg cgatcacagt acctttaccg cccatcaggt catcaaagag gtaacggacg
     5641 atcagttcgc ccatctggta gttgtcgctg gtagcattga cctgcattcc atcaataaaa
     5701 ccgctgtcgc agccgaatac agggatatct gcgtcaaagg cttctgtcag ctgattggca
     5761 acctggtttg ggtcagcact tactaaaaca atgccgtcag tgccggaaga aaccacatct
     5821 tcaatacggc ttgcaagctc tgcaaaatca ttggcagtat cgatcacgaa tacttctgcg
     5881 ccttctgcct ctaagttatt cttgataaag ccagccatct ggttggtggt aacagaagta
     5941 agataagggg tcataacaga gatcttttta ccggcaagag cagtactgtc agctgttttt
     6001 gtgtcagctt cgccggaagc ggcatcttca gaagcttttt cggtagtgga agcagcttct
     6061 gtctgtgcag cagtctcagc ctttggggca gttgtttcat ctgcttctgt agagcagcct
     6121 gtaagcagga tgccggtcag cgctgcagct gcagccaggg ttaagaaatt acgcattttt
     6181 ttcatagtgt tctcctcctg aaataaaatt tttgtttgtt ggggaataaa tctgggaata
     6241 aaaaatcccg gacatgactt ttacatcatg tccgggacga attgttcgtg gtgccacccg
     6301 aattcacaga tcttgtttaa catcagggtt ccaaaatcac aaatcctctt acaagcaagc
     6361 ttgtatcgga tttttgactt ttgaaccctg atgttaacaa aaatagcagc tatcccataa
     6421 tcggaatatc tgccaccaat ctgtctcatt ttcagagtac ggaaagtaaa aaataaaact
     6481 tcgatactct tatcctttaa cgcaggaatc acggcctcag ctacttgcta ttgcgttcgc
     6541 ctggcgtctc acaaacccat tccataagga actccatact gctttccacc accggcagct
     6601 ctctgtgata tcattccaga tgtactctct ttgctcatcg acgttgcttt taaattttat
     6661 ggtaataaag tagcacaatt tatattgact gtcaattata aatttaggga tttttaagtt
     6721 tgatttataa gtggatttca aaaggttttc taaagattgc cggattgcat tgaccgaatg
     6781 gttgctatgc tgtaacatgg tagtaacgga ggagcaagtt cgatccgcaa aaataaaaaa
     6841 cggaggaaca ttagatgaca agagcagaac tgaaacggaa cgcaagggag aaacttgggg
     6901 gacagctgtt cgggcctaac tgggtcaatg cagtccttgt aatggccatt ttttatatac
     6961 tgacgggagc cgtaaacggt atcgcaggat ttgtaaccct gatcatgtta gtgatcggag
     7021 gccctttaag ctacggagtg gcaaaattgt ttttacagca gtgtgatgac ggacagaaaa
     7081 tgaacccgac agaagtattt aagggctttt cagaagactt tggcggaagc tttatcttat
     7141 atctgttaag ataccttttc attgccttat ggtccatttt gctgatcatt ccgggcattg
     7201 taaaaatgta ttcttactcc atggcatttt ttataaaagc agatcatcca tcatatgact
     7261 ggagagaatg cttagatgcc agcagccagc tgacatatgg acacaaatgg gaactgttta
     7321 ttctggatct gagttttatt ggctggcaga ttgtcggaag cttgtgtctg ggtattggaa
     7381 ctttctgggt aaacgcttac agagaggcta ccattgcgga atactacaga tattatgaaa
     7441 gcaatcaggt gatcgacaga gatttttaaa atataatatt tcataaacag atcaaagggc
     7501 acctcacagc gaggtgccct ttttaagcgg atcatttcgg atcattttta attactgctg
     7561 agccttatac tgctctaaag cggttgccag ctggtcagga caggaagttg ggcgagggcc
     7621 gcacttgatg cctttcatac gtttgatggc ttcatcgata tccattcctt caaccagtgc
     7681 tgcaacgccc tgtgtattgc cggagcagcc gcctacaaaa tgaacattgg ttaccttatt
     7741 gtcttttact tcaaaagaaa tagctcttga acagacgccg tgggttgtat aattcatcac
     7801 atgtacctcc ttggggttta gttttgtaaa taagtatggc agcagaaaag tatgccgccc
     7861 ggttcttgac gggcagaagc caaactgtta ccatgtatct gtgtacattt ttatttgtac
     7921 acatgtataa cgttacggtt catgttg
//
LOCUS       DBMDUA010000132         7942 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4062_length_7942_cov_3.320750, whole genome shotgun sequence.
ACCESSION   DBMDUA010000132 DBMDUA010000000
VERSION     DBMDUA010000132.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4062_length_7942_cov_3.320750"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            96..308
                     /locus_tag="ACZPCL_09800"
     CDS             96..308
                     /locus_tag="ACZPCL_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007888443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6219 family protein"
                     /protein_id="HIR1162795.1"
                     /translation="MKAHKYWSVGALITMIGTFYTGYKGSKSSHRYFACSALICMIMS
                     MYTGHKMISGNKRTRKQVKKTEAEES"
     gene            309..764
                     /gene="bcp"
                     /locus_tag="ACZPCL_09805"
     CDS             309..764
                     /gene="bcp"
                     /locus_tag="ACZPCL_09805"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HIR1162796.1"
                     /translation="MLELGTKAPAFELPDQNGEMHKLSDYLGKKVILYFYPKDNTAGC
                     TKQACGFSERYPQFTEKGAVVLGVSKDSVASHKRFEEKYGLAFTLLADPERKVIEAYD
                     VWKEKKNYGKVSMGVVRTTYLIDEQGVIIKANDKVKAADDPENMLKELS"
     gene            764..1516
                     /gene="yaaA"
                     /locus_tag="ACZPCL_09810"
     CDS             764..1516
                     /gene="yaaA"
                     /locus_tag="ACZPCL_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HIR1162797.1"
                     /translation="MKIILSPAKKMIVDTDNIAPVELPVFIDKTEEILSWMKSKSKEE
                     LQTIWKCNDKIAEQNFNRLENMDLYNRLTPAVLAYEGIAFQYMAPSVFEIQQFEYLQN
                     HLRILSAFYGILKPMDGVTPYRLEMQAKAEIEETTNLYDYWGDLLYHSVIDDSRTIIN
                     LASKEYSKCIEKYLTPQDRYITIVFCELSGDKLVTKGTYAKMARGEMVRFMAENNIEN
                     PVDIKKFDRLGYTFRGDLSSETEYVFERKAIK"
     gene            1647..1805
                     /locus_tag="ACZPCL_09815"
     CDS             1647..1805
                     /locus_tag="ACZPCL_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0043448 - alkane catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HIR1162798.1"
                     /translation="MKYVCDVCGWEYDEEKGYPEGGIAPGTKWEDVPADFECPLCFVG
                     KDQFSKAE"
     gene            2157..2384
                     /locus_tag="ACZPCL_09820"
     CDS             2157..2384
                     /locus_tag="ACZPCL_09820"
                     /inference="COORDINATES: protein motif:HMM:NF018926.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HIR1162799.1"
                     /translation="MAYEYDHDCPFEAYITNLGKYNEGELVGEWVKFPTTSEDLQKVF
                     ERIGIGSKDDFGNPYEEWFISDYDVYGGGLP"
     gene            2546..2989
                     /locus_tag="ACZPCL_09825"
     CDS             2546..2989
                     /locus_tag="ACZPCL_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024571.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1162800.1"
                     /translation="MKKLQFLKAGMESGVSPAQFISINFEDLNYAYLQTAKALHNEIT
                     KRATEINGKAYLFFDEIPEVKDWEKCINSLRVSLDCDIYITGSNAKLLSSELSTYLGG
                     RYVEFIIYPFSFAEFLELYRPIAPDEPIQKCFQKYLLSGTFAPGL"
     gene            2973..3239
                     /locus_tag="ACZPCL_09830"
     CDS             2973..3239
                     /locus_tag="ACZPCL_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1162801.1"
                     /translation="MRRDYKVTVGKTGNKEIDFVCDKRGEKLYVQVTYLLASDETIQR
                     EFGAYDNIRDNYPKYVVSLDEFDMSRNGIKHRNIRDFLLAEEWN"
     gene            complement(3335..4159)
                     /locus_tag="ACZPCL_09835"
     CDS             complement(3335..4159)
                     /locus_tag="ACZPCL_09835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1162802.1"
                     /translation="MAMNIDFTGKTVLVTGGGRGLGKEMALQFAGCGANVYIANRKED
                     QGLATVKEIEALGVKGGFTKCDVAVEEDVKKLIADAAAFGGGKIDVIVNAAGVISLQD
                     MMHTTTEEVQRLFNINVVGTVHMIKHGLAHLEANKGGNMILVSSIAGRDGMGMLQTYS
                     ASKAAVTSLMQSAAKHAAPYGVRVNAILPGIIRTAMWEEILEGMNNGWDPEVHNELTP
                     EKREELWNASVKSMIPLGHDQKEEDIAWATVFLASDFAREITSQALCIDGGTTSGR"
     gene            complement(4298..4912)
                     /locus_tag="ACZPCL_09840"
     CDS             complement(4298..4912)
                     /locus_tag="ACZPCL_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162803.1"
                     /translation="MFGLEIATLLLGISFIAAGMIQSRKTGQKKWRILMIIGIIPVLV
                     SVFLLVCTFWLLYGIQVQEPVAGPTENGKIITENTDAEPEPGSGSDSNIPGSDWRTYR
                     AYSEDLHLDDTGALIFCLSPLDNRNGYAVYDSANGARVGTLLWGSEKEADWQAKAQME
                     DLDHDGIKEIGISMADGEILWYHYREGLEDTWPENKCGCFEKVD"
     gene            5115..5582
                     /locus_tag="ACZPCL_09845"
     CDS             5115..5582
                     /locus_tag="ACZPCL_09845"
                     /inference="COORDINATES: protein motif:HMM:NF015862.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HIR1162804.1"
                     /translation="MYDLKMLEEIKSQEERIHFSSFDYNDAYTLGTMLRERGLTTPKP
                     IAIRIVFDDIILYQSFLPGTDESNNQWMNRKQHTVERCHTSSLRAAVERELNGVKENW
                     QQDESHYAFCGGGFPIIVNDEFRGVAMISGLPHLEDHRNLVEVMDQFCAQKNA"
     gene            complement(5710..6051)
                     /locus_tag="ACZPCL_09850"
     CDS             complement(5710..6051)
                     /locus_tag="ACZPCL_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIR1162805.1"
                     /translation="MNIQIFGTKKCNDTKKAERFFKERGIKYQFIDMKEKGMSKGEFN
                     SVAQANGGLENMINWDGKDKDILSLIKYIAEEDKLQKVLENPQVIKTPVVRNGKQSTL
                     GYQPDVWKKWE"
     gene            complement(6142..7206)
                     /locus_tag="ACZPCL_09855"
     CDS             complement(6142..7206)
                     /locus_tag="ACZPCL_09855"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF014477.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR1162806.1"
                     /translation="MKKWCLVILALMLCGCSASTKTAETEAVKNDRPVLRTETGSEEK
                     NFTVFAMDTVMQFQIYGGNDDVEEKVRGKIEELEDTLSVTKEGSPMWNLNEKKNENFA
                     EEFANLMLQSLKLCKDTDGALDISAYPIVKAWGFTTGEHRIPDEEERKALVSRVDYRK
                     IQVNGNTVSIEPDMMVDLGSVGKGYTAEVLANLMKEEGVTSALFSLGGNIQTVGTKSD
                     GSDWFIGIRAAEEDRYLGGVSVTDKAVVTSGGYERYFTGDDGQVYWHIMDPKTGAPAK
                     NGLVSVTIISDSGFLCDGLSTALFVMGPEKAAEHWRSHQNYEAVLEDDKGQIYITEGL
                     ENTFKPVDLGTEQEIQVIRR"
     gene            complement(7207..>7942)
                     /locus_tag="ACZPCL_09860"
     CDS             complement(7207..>7942)
                     /locus_tag="ACZPCL_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162807.1"
                     /translation="FLAKYEAYRLLLKVDTINDISRDTYLGAIDYIMVGQHQYESCSY
                     GSISDQQFKVGTTPESDGMGKGKDYIDFAKDGFDAYETGDLIKVTIYSKKSEYGSYVF
                     YVRDGELVDPTEAEEELLDEQEMKEEAQEEVTVPETKDETVVPEVPADKDAEEVPAED
                     EVTEPSEEDKEDTAEEPAEDKPEEDASEDKDSEDKDTEETPAEEDKEDASEDNNSEDS
                     NEDVSEDNAADDVAEDTEATETEGEI"
BASE COUNT         2211 a         1683 c         1588 g         2460 t
ORIGIN      
        1 gtaacgcctg aaagtcataa gacagcaata gaagcaatga agatgatcca gagatattta
       61 cattggaaat agcttaatca atttggaggt atatcatgaa agcacataaa tattggtcag
      121 tcggtgcgtt aattactatg attggaactt tttataccgg atataaagga tctaaatcaa
      181 gtcataggta ttttgcgtgt agcgctctta tatgtatgat tatgtccatg tacactggac
      241 ataaaatgat ttcaggtaat aagagaacga gaaaacaggt gaaaaagaca gaagcggagg
      301 aatcataaat gttagagtta ggaaccaaag caccggcttt tgaattgccg gatcaaaacg
      361 gagaaatgca taaattaagt gattatttag gtaaaaaggt aattttatat ttttatccaa
      421 aggataacac ggcaggctgt acaaagcagg catgtggttt ttccgagcgt tatcctcaat
      481 tcactgagaa aggagcagtt gttcttgggg taagtaagga ttctgtagcc tctcacaaga
      541 gatttgagga aaaatatgga ttggcattta cacttttggc agatccggag cgcaaggtga
      601 tcgaagcgta tgatgtatgg aaagaaaaga aaaactatgg aaaagtctct atgggcgtgg
      661 taagaaccac atatcttatt gatgagcagg gagttatcat caaagccaat gataaagtca
      721 aggcggcaga tgatcctgaa aacatgctca aggaactgtc ataatgaaga ttattttatc
      781 acctgcaaaa aagatgattg tagatacaga taacatagca ccggttgaac tgcctgtctt
      841 tattgataag acagaggaga tattgagctg gatgaaaagt aaatcaaaag aggaactgca
      901 aactatctgg aaatgtaatg acaagattgc ggagcagaac tttaacagat tggaaaatat
      961 ggatctttat aacaggctta ctccggctgt tttagcatat gaagggattg catttcaata
     1021 tatggcacca tcggtgtttg aaatccagca gtttgagtat ttgcaaaatc atttaagaat
     1081 cctgtctgca ttttatggca ttttaaaacc aatggatggt gtgactcctt atcgtcttga
     1141 aatgcaggca aaagctgaga ttgaagaaac aacaaatcta tatgattact ggggtgacct
     1201 gctatatcat tcagtgattg atgatagcag aacgatcata aatctggcat ctaaagaata
     1261 ctcaaagtgc atagaaaagt atctgacacc acaggacaga tatattacga ttgtattttg
     1321 tgagttatcc ggagataaat tggttacaaa aggtacatat gccaaaatgg cccgcggtga
     1381 aatggttaga tttatggcag agaataatat tgaaaatcct gtagatataa aaaagtttga
     1441 tagacttgga tatactttca gaggagatct gtcatcggag acagagtatg tttttgaacg
     1501 aaaagccata aagtaaaatc gtgatagaag aacataacgt agaaatgttc attgcaaaaa
     1561 tgataataat tactattatt atcttgacta tcatgtatta ttgttatata cttttataaa
     1621 gaataaattc tatggaggaa ctggtcatga aatatgtatg tgatgtttgc ggatgggaat
     1681 atgatgaaga gaaaggatat ccagaaggtg gtattgcacc aggaacaaaa tgggaagatg
     1741 ttccggcaga ttttgaatgt ccattatgct ttgttggaaa agatcagttt tctaaagcag
     1801 aataaataat caagaagaag aggaaggatt tttttatgga attattatat gtgtagagtc
     1861 tgtggacaga ggatagggga atgtgcagca tgtttccgta ttgctcccat tttgttacca
     1921 attgctatcc ttttactacc attctgcctc tttttttcag aattgacctg tgctaatatt
     1981 atcatagaca aatgagacat gagctacaaa cttacatttc atttgtttct cttttgcaaa
     2041 agaatacgga caaaatataa agcgtctgct cattgccgga atggtgatgg gtatggcgct
     2101 ttttttgatg ccgaaaaccg ggtgacgacc tgaaaaatga aacaggagga aatagcatgg
     2161 catatgaata tgaccacgat tgcccatttg aagcttacat cacgaatctc ggaaaatata
     2221 acgaagggga gctggtaggc gaatgggtaa agtttcctac cacgtcagaa gatctgcaaa
     2281 aagtatttga acgaattgga atcggttcaa aagatgactt tggtaatcct tatgaagaat
     2341 ggttcataag tgattatgat gtgtacggcg gcggactgcc gtgaacctgt gtaagcagaa
     2401 taggcttgat gcctggggca gccttcgggt tgcctttaca tagtttccac tttcaaaaaa
     2461 taaaaaaatt tgcgtttttg aaagcgacat cttgcatata ctattagcag acagtataat
     2521 acgtacagcg aagcggtttc gagaaatgaa aaaacttcaa tttttgaaag cgggtatgga
     2581 gtccggtgtc agtcccgctc agttcatctc tattaatttt gaggatttaa actacgcata
     2641 tctgcaaact gcaaaggcac tgcataatga aatcacaaaa cgagccacag aaatcaacgg
     2701 taaagcctat ctgttctttg atgaaatccc ggaagtaaaa gactgggaga agtgtatcaa
     2761 ctccctgcgt gtatcccttg actgcgacat ctatatcacc ggttcaaacg caaagcttct
     2821 ttccagtgag ctttccactt atcttggcgg acgttatgtg gagtttatta tttatccatt
     2881 ctcctttgca gagttcctgg aactgtatcg ccccattgcg ccagatgaac cgatccagaa
     2941 atgctttcaa aagtatcttt tatctggaac ttttgcgccg ggactataaa gttacagtag
     3001 gcaaaaccgg aaacaaggaa attgactttg tatgcgacaa acgcggggaa aaactgtatg
     3061 ttcaggttac ctatttgctg gcatccgatg aaaccatcca gcgtgagttt ggagcatatg
     3121 ataacatccg tgataactac ccgaaatatg ttgtctctct ggatgaattc gacatgagca
     3181 gaaacggaat taaacaccgc aacatacggg atttcctact ggcagaggaa tggaattaaa
     3241 aaaatagccg ctaatggaat tcattttcca aaagcggctg ctctttttat ctctttattt
     3301 ctttaattta aatttttcaa ttcatgtcat atgattatct tccggaggta gtaccaccat
     3361 caatgcacag tgcctggctt gtgatctcgc gggcgaagtc acttgcaagg aatacggttg
     3421 cccaggcaat atcttcttcc ttctgatcat gtcccagcgg gatcatgctc ttaacggaag
     3481 cattccacag ttcttcacgt ttctcaggag tcagttcatt atgaacttct ggatcccagc
     3541 cattgttcat gccttccagg atctcttccc acattgctgt acggataatt cccgggagaa
     3601 tagcatttac acggactccg taaggagctg catgttttgc tgcggactgc atcaggcttg
     3661 ttacagctgc cttggatgcg ctgtaagtct ggagcattcc cataccgtct ctgcctgcaa
     3721 tagaggatac aaggatcatg tttccgccct tatttgcttc cagatgtgcc agaccatgtt
     3781 tgatcatatg tacggttccc actacattga tattgaacag tctctgaacc tcttctgtag
     3841 ttgtatgcat catatcctga agggaaatta cgcctgctgc atttacgatc acatcgatct
     3901 ttccgccgcc aaatgctgct gcgtcagcga tcagcttctt aacgtcttct tccacagcta
     3961 catcacattt tgtaaagccg ccctttacgc caagtgcctc gatctctttt acagtagcaa
     4021 ggccctgatc ttctttacgg tttgcaatat atacgtttgc gccacatcct gcaaactgaa
     4081 gtgccatctc tttacccagt ccacgtccac cacctgtaac aaggactgtc tttccagtaa
     4141 aatcaatatt cattgccata accaaaatcc cctttccaga tatatgttct gtttaactct
     4201 gttaatattt tatcactctg tatggaaatg tcaatgttta taatcttaag tttttacagt
     4261 ttttcccata attacaggct tttcttttgt taatttttta atcaactttt tcaaaacaac
     4321 cacatttatt ctccggccag gtatcctcca aaccttcccg gtaatggtac cacaggattt
     4381 ctccatctgc catggatatg ccgatttctt ttatcccgtc atgatctaag tcttccatct
     4441 gtgctttagc ctgccagtcc gcttcttttt ctgatcccca taaaagagtt cctactctgg
     4501 caccgtttgc gctgtcatat acagcatagc cgtttctatt atctaatggg gaaaggcaga
     4561 aaatgagagc gcctgtatca tccaggtgca ggtcttcgga ataagccctg taagtgcgcc
     4621 agtcgctgcc agggatgttg gaatctgaac cggaacctgg ttctggctct gcatctgtgt
     4681 tttccgtaat gatctttccg ttttctgttg gacctgcaac cggctcctgg acctggatcc
     4741 catataagag ccagaatgtg cagaccaata aaaatacact gaccagaacc gggatgatcc
     4801 caatgatcat taaaatacgc cattttttct gccccgtctt tctgctctgg atcatgcctg
     4861 ctgcaataaa actaattccc aatagcaatg tggctatttc caatccaaac atccattttc
     4921 cctctttctt acaaattatg aaactgatcc tattgttctg tatatctgaa ctatacagct
     4981 gttccttcag ggaaaatatt aactaagggt tagttttaga aacagttttg gggactttat
     5041 ttttttacta tacacatttc tatttaaagg ctataataat tctggaaaaa tatgagtata
     5101 gagagggtat tcttatgtac gatttaaaaa tgttagaaga gatcaaaagc caggaagagc
     5161 gtatccactt ttcttctttt gattacaatg atgcttacac actgggaacc atgcttcgcg
     5221 agcgtggtct gaccactcca aagccaatcg ccatccgtat tgtttttgat gatattatcc
     5281 tgtatcagtc cttccttccg ggaacagacg aaagcaataa ccagtggatg aaccgcaagc
     5341 agcacacggt tgagcgctgc catacttctt ctctgcgtgc agctgttgag cgggaattaa
     5401 acggtgtaaa ggaaaactgg cagcaggacg agtcccacta tgcattctgc ggcggtggat
     5461 ttccaattat tgtaaatgat gagttccgcg gtgttgccat gatctccggt ctgcctcatc
     5521 tggaagacca cagaaatctg gtggaagtaa tggatcagtt ctgcgcacag aagaacgcgt
     5581 aagatctatt cctattattg gttctcacag caaatataaa acaggtcgcg gatatttcca
     5641 taaaatgtga tatctgcgac ctgctttttt attatacgca tttttctgct atttttatct
     5701 attacctatc tattcccatt ttttccacac atccggctga tagcctaagg tagactgctt
     5761 tccgtttctg accaccggtg ttttgatcac ctggggattt tccaggactt tttgcagctt
     5821 atcttcctct gcgatatatt tgatcaggga caaaatatct ttgtctttcc catcccagtt
     5881 gatcatattt tccaggcctc cgtttgcctg ggcaacggag ttaaactcgc cttttgacat
     5941 gcctttttcc ttcatgtcaa tgaactggta tttgatgcca cgctctttga aaaagcgttc
     6001 tgctttcttt gtatcattgc acttttttgt gccgaagatc tggatgttca taaaatatgt
     6061 ccgccttttt cctgaaaatc tgtgttttta ttcatatctt cactcatgat accatgctgc
     6121 atttgtcttt acaagccgtc tttatctgcg gatcacctgg atctcctgct ctgtgccaag
     6181 atctaccggt ttgaaggtgt tttccaggcc ttctgtaata tagatctggc ctttatcatc
     6241 ttctaagact gcctcgtaat tctggtggct gcgccagtgt tcagccgctt tttccggacc
     6301 catgacaaaa agggcagtgg aaagaccatc acagaggaaa ccgctgtcac tgataatggt
     6361 cacagatacc agcccatttt tagccggagc accggtttta ggatccataa tgtgccagta
     6421 gacctgaccg tcatcacctg tgaaataacg ctcgtagcca ccggaagtaa ccaccgcctt
     6481 atctgttaca gaaacacctc ccagataacg gtcctcttct gctgcccgga taccgatgaa
     6541 ccagtcagac ccgtccgact ttgtacctac tgtctggata tttcctccca gactgaaaag
     6601 agcagaagta acaccttctt ctttcatcag gtttgccagg acctctgctg tatagccttt
     6661 tcctacactt cccaaatcta ccatcatatc cggttctatg gatacagtat taccatttac
     6721 ctggatcttg cggtaatcta cgcgggagac taatgctttg cgttcttcct catcggggat
     6781 gcgatgttca cctgtggtaa agccccaggc ttttacaatg ggataggcgg aaatgtctaa
     6841 ggcaccgtct gtatctttgc aaagttttag tgactgaagc atcagatttg caaattcctc
     6901 tgcaaagttc tcattcttct tctcgtttaa attccacatg ggacttcctt cttttgtcac
     6961 agaaagtgta tcttccagtt cttctatttt cccccggact ttttcttcta cgtcatcatt
     7021 tccgccatag atctggaact gcatcactgt gtccatggca aatacggtaa agttcttttc
     7081 ttcggatcct gtctctgtac gcaaaacagg gcggtcattt ttgaccgcct ctgtttcagc
     7141 tgtttttgta gatgcactgc atccgcataa catcagtgcc aagatcacca gacaccattt
     7201 tttcaattag atttctcctt ctgtttcggt tgcttctgta tcttcggcaa catcgtcagc
     7261 tgcattgtct tcggaaacat cttcattgga gtcttcggag ttattgtcct cggaagcatc
     7321 ttctttatct tcttcagctg gagtttcctc tgtatcctta tcttcggagt ccttatcctc
     7381 agatgcatct tcctctggct tatcctctgc cggctcttct gcagtatctt ccttatcttc
     7441 ctcagaaggt tcggttactt catcttctgc cggaacttct tctgcatcct tatcagccgg
     7501 aacttccgga acaactgttt catcctttgt ttcaggaaca gttacttctt cctgagcctc
     7561 ttctttcatt tcctgctcat ctaaaagttc ttcctctgct tcggttgggt ctaccagttc
     7621 gccatctctt acatagaata cataagaacc atattcgctc ttcttactgt aaatagtaac
     7681 tttgatcaga tcacctgttt cgtaagcatc gaaaccatcc ttagcaaaat caatgtaatc
     7741 ttttcctttt cccataccat cactttcagg tgttgtacct actttaaact gctgatctga
     7801 tatagaacca taactacagc tttcatactg atgctgtcct accattatat aatctatagc
     7861 accaagatag gtatctcttg aaatatcgtt tatggtgtct actttaagga gtaaacgata
     7921 tgcttcatac ttcgccagga aa
//
LOCUS       DBMDUA010000133         7895 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4085_length_7895_cov_3.115345, whole genome shotgun sequence.
ACCESSION   DBMDUA010000133 DBMDUA010000000
VERSION     DBMDUA010000133.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7895)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4085_length_7895_cov_3.115345"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(96..1550)
                     /locus_tag="ACZPCL_09865"
     CDS             complement(96..1550)
                     /locus_tag="ACZPCL_09865"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR1162808.1"
                     /translation="MYRKSARGWEKHLDFMIWDQLSLQIAFLLAYMLRNGLSNPYSHL
                     IYCNVAFILVVIDAGVLLLFESLKNVLKRGYYQEFAMTVKHVILVILVTTFYLFYIKR
                     SEDYSRLALLYMTVLYLLVSYLTRILWKDQLKRKMRSSMKRSLLIITTKSMLDTVVYN
                     IRNFNYEMFHVAGVAVIDCDMVGHDCEDSITVVANADTVADYVCREWVDEVFVNLPDD
                     APYPEMLLNQLTEMGAVVHMKLAKTSNLIGEKKFVERLGIYTVLTTSINYVTAKQLFM
                     KRALDILGGLVGCLITLILTVILGPLIYIQSPGPIFFSQVRVGKNGKKFKIYKFRSMY
                     MDAEERKKELMKQNRVKDGMMFKLEWDPRIIGTKKLPDGTIKKGIGNIIRDFSLDEFP
                     QFFNVLKGDMSLVGTRPPTVDEWDKYDLHHRARLATKPGITGMWQVSGRSNITDFEDV
                     VKLDRQYIAEWNFALDIKILFKTVLVVFKRDGAM"
     gene            complement(1625..2338)
                     /locus_tag="ACZPCL_09870"
     CDS             complement(1625..2338)
                     /locus_tag="ACZPCL_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162809.1"
                     /translation="MITMKKKALVAMAMAAAMVAMPVVAGAVTSPGSQSDGTIGQDSS
                     SQSQNNTNTGSQQGEQNSDTNAGSNAGSGVNKVAEATVVQGRDNERQEVTNVTKDGSE
                     VTMGLIEDASNSDVDQSIATGEAETAGLGDESVSQINGLNNGTKKASDFVPNAGLEEY
                     ANVGKTRAIISQKNGHDVRSTVNLYVSTLTAGKDIVIIYLDNNTSNWVTVKNPTINWN
                     TKYVSFEVPGSCTVQVLAK"
     gene            complement(2410..3522)
                     /locus_tag="ACZPCL_09875"
     CDS             complement(2410..3522)
                     /locus_tag="ACZPCL_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1162810.1"
                     /translation="MKKKIYSALIGIGLVSLTLGIWGGVTMYKNRQATELIIQARNEE
                     RQKAEAESQTAAEEEIGIAPGQKEELTADPRDLNLVPILAQDQLTYKGKNYRRNQYVK
                     AILCMGVDRSDTMTETKELGLAGQSDGIFLIAQDTARHTLKILMIPRDTMTEIPITNK
                     ERTLHDTKITQLTMAYAYGDGREGSCENMVESVQKLLCGFTIDQYMAVDTTVVAQLND
                     AVGGVTVTVPTEGMEKKDPAFVKGNQVTLHGKQAEAFVRYRDTDYHFTALYRMDQQQE
                     YITQYFQSVKAASKTDSQIVVHLFDMIQDYMVTDMTKDQYVKIAMDGMEAGSLTSEDF
                     YTVPGVEEITEKYDVYRADRQAAIQIILDLFYRETE"
     gene            complement(3552..4316)
                     /locus_tag="ACZPCL_09880"
     CDS             complement(3552..4316)
                     /locus_tag="ACZPCL_09880"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HIR1162811.1"
                     /translation="MNNKVISLKREIKDDYNYRESLKTLRTNIQFCGSNIHTIMFTSA
                     LPDEGKSDIAFSVAASFAQIGKKVIFIDADIRKSVLVSRYQLEGEVNGLSQYLSGQKN
                     REDIIYETNYEGFSVIFAGPYSPNPAELLEEDLFHDLIQSLKDYDYVIIDTPPMANLI
                     DSAIVASNCDGAIIVVEQGAVSYRLEQKVKSQLDKTGCRVLGVVLNKVDLSQNGYYGR
                     YGKYGKYGKYGRYGKYDKYGAYSPYEKAEAAQTTDK"
     gene            complement(4313..5101)
                     /locus_tag="ACZPCL_09885"
     CDS             complement(4313..5101)
                     /locus_tag="ACZPCL_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0061097 - regulation of protein tyrosine
                     kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YveK family protein"
                     /protein_id="HIR1162812.1"
                     /translation="MDNKTINMNDDEVEIDLLQLFRALKKRILWIVGAGVIMGGAFGL
                     FSKFAITPQYTSTSMVYILSKETTLTSLADLQIGSQLTQDYKIIVTSRPVISEVIDKL
                     KLDISYENMVSKITINNPNDTRILSITAQDPDPVMAKDIADSVAKTSSEYIGDIMEMV
                     PPKVIEEGTVSDKKASPSNGKNAMIGAMLGIVLVCGLTVVEELLNDTIQTEEDVEKYM
                     GLTVLASVPLREGEAEENKNEQTLSRSHMHNKDDKKKGKQGDRK"
     gene            complement(5129..5848)
                     /locus_tag="ACZPCL_09890"
     CDS             complement(5129..5848)
                     /locus_tag="ACZPCL_09890"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HIR1162813.1"
                     /translation="MKVRQRIIDVHAHVIPGVDDGSRTMEESVEMLKRAADQGIVGVI
                     ATPHYSRRHVLKGLNDSAKLLQQEIRKTYPKFRIFPGQETFYHDELAQRLNEGKAYTM
                     ADSQYVLVEFMPSVSYEILFQGIRKLVSNGYTPILAHMERYGCLRKKGTSELLDIGCK
                     LQMNYDSIQGHWFSPEVRWCREQVKSGVIHFLGTDMHRSDYRPPQITEALKWLDGHID
                     AQQIWCITYQNALRVIKKETI"
     gene            complement(6138..6668)
                     /locus_tag="ACZPCL_09895"
     CDS             complement(6138..6668)
                     /locus_tag="ACZPCL_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HIR1162814.1"
                     /translation="MGFLLFLSDFMVPLIIFYIVGFAILGKRPVFDDFLYGAKEGMQT
                     VVQVLPSLVGLMTAVGVLRASGFLEFLTHILEVPATSIGLPGPIIPIAFIRLVSNSAA
                     VGLVLDLFKNYGPDSQVGLMASILMGSTETVLYCMSVYFGSAGISRTRYTLAGGLLAA
                     FVSLAASVILANQLAL"
     gene            complement(6807..7742)
                     /gene="cysK"
                     /locus_tag="ACZPCL_09900"
     CDS             complement(6807..7742)
                     /gene="cysK"
                     /locus_tag="ACZPCL_09900"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR1162815.1"
                     /translation="MAGIVKGAAGLVGNTPLLEPVNLEKKEGLKARILLKLEYFNPAG
                     SVKDRVGKAMIEDAEARGVLKPGSVIIEPTSGNTGIGLASVAASKGYRVILTMPETMS
                     VERRNILKAYGAEIVLTPGDKGMSGAIAKADELAKEIPDSFIPGQFVNPVNPKIHKET
                     TGPEIWRDAEGQVDFFVAGVGTGGTITGVGTYLKEQNPNVKVVAVEPATSPVLSEGHG
                     GAHKIQGIGAGFVPKVLDTKIYDEVMPIDNEHPFEASKLLAKTEGILTGISSGAALYA
                     AIQLAKRPENEGKTIVALLPDSGDRYYSTPLFTEA"
BASE COUNT         2021 a         1853 c         1518 g         2503 t
ORIGIN      
        1 ctcattttta tccagttcgg tgaacttcat gaccgcttta tgatcatttt acgaagccac
       61 cttgtataca attagtatac cattaatttg tgatcctaca tcgcaccgtc ccgtttgaat
      121 acaaccagta cggttttgaa aaggatcttt atatccagag caaagttcca ctctgcaatg
      181 tactgcctgt cgagttttac tacatcctcg aagtcagtga tgttgcttcg tccgctgacc
      241 tgccacattc ctgtaatgcc aggtttggtg gcaaggcggg cacggtggtg aagatcatat
      301 ttgtcccatt catctaccgt cggcggtctt gtgcctacaa ggctcatgtc accttttaat
      361 acgttaaaga actgtgggaa ttcatccagg ctgaagtctc ggatgatatt tccaattcct
      421 ttctttattg taccatccgg aagtttcttg gttccgatga ttcgtggatc ccattccagt
      481 ttaaacatca taccgtcttt tacacggttt tgtttcatca gttctttctt ccgctcttct
      541 gcatccatat acatactgcg gaatttatag attttgaatt tctttccatt ttttcccaca
      601 cggacttggg aaaagaaaat cggtccggga gattgtatgt agatcaacgg tcctaagatc
      661 acggtaagga tcaatgtgat caggcaccct accagtccgc ctaaaatatc taaagcacgt
      721 ttcataaaaa gctgcttggc tgttacataa ttgatactgg ttgtcagtac tgtgtagatt
      781 cccaggcgtt ctacaaattt cttttcgccg atcaggttgg atgtttttgc cagcttcata
      841 tgaacaaccg cgcccatttc tgtcagctgg tttaacagca tttccggata cggagcatcg
      901 tctggaagat ttacaaatac ttcatctacc cattccctgc agacgtaatc cgctactgtg
      961 tctgcatttg ctacaactgt aatgctatct tcacaatcat gccctaccat atcgcagtcg
     1021 attactgcca cacctgcaac atgaaacatt tcataattaa aattgcgaat attatagacc
     1081 accgtatcca gcatgctttt tgtagttatg atcaacagag aacgtttcat gctggaacgc
     1141 atttttcttt tcagctggtc tttccacaag atacgggtca gataagaaac aagtaaatac
     1201 agaaccgtca tataaagcaa tgccagtctg gaataatcct cgcttctttt tatatagaaa
     1261 agataaaacg tagttaccag gatcaccagg atcacatgtt ttactgtcat tgcaaattcc
     1321 tgataatatc cacgttttaa cacatttttc aaagattcaa agagtaatag tacgccggca
     1381 tcgatcacaa ctaagataaa tgccacgtta cagtatatta aatggctata gggattacta
     1441 aggccatttc ttaacatata agccaataaa aatgcgatct gtaaactcag ttggtcccag
     1501 atcataaaat ccagatgttt ttcccatcct ctggcgcttt ttcgatacat atccccactt
     1561 catccttttt cgtttgcgtt tataacagag ccggaaatgt ctcccggctc catcataata
     1621 gttgttattt tgcaagaacc tgtacagtac aagaacctgg tacttcaaaa cttacatatt
     1681 ttgtattcca gttaatggta ggattcttaa ctgtaaccca attgcttgta ttgttgtcta
     1741 agtagataat tacaatatct ttgcctgcgg ttaaggtact tacatacaga ttaactgtag
     1801 aacgaacatc atgtccattt ttctgagaga tgatcgcacg ggttttacct acattagcat
     1861 actcttccag acctgcatta ggaacaaaat cactagcctt tttggttccg ttatttaagc
     1921 cattaatctg acttacagat tcatcaccaa gtccggcagt ttctgcttca ccagttgcaa
     1981 tactctggtc aacgtcacta ttggaagcat cttcaatcaa tcccatagtt acttcagaac
     2041 catcctttgt aacattggtt acttcctgtc tttcgttgtc tcttccctgg acaacagttg
     2101 cttctgctac tttgtttact cctgatcctg cattggatcc agcattagta tcggagttct
     2161 gctctccctg ctggctgcca gtattagtat tattctggct ttggcttgat gaatcctgac
     2221 caatagttcc atcagactga ctaccaggac ttgtaactgc accagctact accggcattg
     2281 ctaccatagc tgcagccatt gccatagcta ctaaagcttt cttcttcatg gttatcacgc
     2341 cttcctccct tttggggtaa aatatatttc aataagcagc gtgtttccac actggatatt
     2401 gtatagtaat tattctgttt cccggtaaaa cagatctaaa atgatctgga tcgctgcctg
     2461 acggtcagca cggtatacat cgtacttctc tgttatctct tctactccgg gaactgtgta
     2521 aaaatcttcg ctggttaaac tgccagcttc cattccatcc attgctatct ttacatactg
     2581 atccttggtc atatctgtta ccatataatc ctgaatcatg tcaaacagat gtaccacgat
     2641 ctggctgtct gtcttggatg cggcttttac tgactgaaaa tactgcgtaa tatattcctg
     2701 ctgctggtcc atacggtaca gtgcagtaaa atgataatcc gtatcacggt aacgcacaaa
     2761 ggcttctgcc tgttttccat ggagagtgac ctgattgcct ttgacaaatg ccggatcctt
     2821 tttctccatt ccctctgtag ggaccgttac tgtaacaccg cctacagcgt catttaactg
     2881 tgctactact gtggtatcaa ctgccatata ctgatcaata gtaaatccgc aaagaagctt
     2941 ctgtacagat tcaaccatat tttcacagct gccttccctg ccatcaccat aagcatacgc
     3001 catagtcagc tgggtgatct tggtatcatg aagggttctt tccttatttg taataggaat
     3061 ttctgtcatg gtatcacgtg ggatcatcag gatctttaat gtatgtcgag cagtgtcctg
     3121 ggcgatcaga aagataccgt ccgactgacc ggccaagccc agctcctttg tctctgtcat
     3181 tgtatcactt ctgtcaacac ccatacagag gattgccttt acatactggt ttcttctgta
     3241 attttttcct ttataggtca actgatcctg ggctaagata ggcaccagat tcagatctct
     3301 tggatctgct gtcaattctt ctttttgccc cggagcaatt cctatttctt cttcagcagc
     3361 tgtctgagac tctgcttcag ctttctgtct ttcttcattg cgtgcctgaa tgatcagttc
     3421 tgttgcctgc cggtttttgt acatagtcac gccgccccag atccccaggg tcagtgaaac
     3481 aagtcctata ccgatcaaag cgctgtatat cttctttttc atatcaatca gcctgatttc
     3541 ctgtgtaact gttacttatc agttgtctgt gctgcttcgg ctttttcata tggactgtag
     3601 gcaccgtatt tatcatactt accatatcgg ccatacttac cgtatttacc atattttccg
     3661 tatctgccat aataaccatt ctggctgagg tcaactttgt ttaacacaac acccagaaca
     3721 cggcagccgg tcttgtccaa ctggctcttt accttctgtt ccagacggta gcttactgca
     3781 ccctgttcaa caacgatgat cgcaccgtca cagttgctgg ctacaatagc actatcgatc
     3841 aggtttgcca taggcggtgt atcgatgatc acatagtcgt aatctttcaa agactggatc
     3901 agatcatgga acagatcttc ttccagaagc tctgccgggt ttggtgaata tggtccggca
     3961 aaaataacgc tgaaaccttc atagtttgtt tcgtaaataa tgtcttctct gtttttctga
     4021 ccactgaggt actgggaaag accgtttacc tcaccttcca gctgataacg ggataccaga
     4081 acagatttac ggatatctgc atcaatgaag atcacctttt taccgatctg tgcaaatgat
     4141 gctgctacag aaaaggcaat atcacttttt ccttcatctg gaagtgcgct ggtaaacata
     4201 atggtgtgga tgttgcttcc gcagaactgg atattggtac gcagggtctt aagagattct
     4261 ctatagttat aatcatcttt tatttcgcgt tttaaagaaa ttactttgtt attcatttac
     4321 gatctccctg ctttcctttt ttcttatcgt ctttgttatg catatgagaa cggctgagtg
     4381 tctgttcatt cttattttct tctgcttcac cttcacgaag aggtacagat gcaagaacag
     4441 tcagtcccat atatttctct acatcttctt ctgtctgaat agtatcattt aacagctctt
     4501 ctacaactgt cagaccacat accagaacaa ttcctaacat agctccgatc atggcattct
     4561 tgccattgct tgggcttgcc tttttatcag atacagtacc ttcctcaatt actttaggag
     4621 gaaccatttc cataatgtct ccgatatact cggaagaagt ctttgcaacg gagtctgcaa
     4681 tatcctttgc catcaccgga tctggatcct gagcagtgat agaaagaatt cgggtatcat
     4741 ttgggttatt aatggtaatc ttgctgacca tattttcgta actgatatcc agtttcagtt
     4801 tatcaatgac ttctgaaata accggacggc tggttacgat gatcttgtaa tcctgagtca
     4861 gctggcttcc gatctgcagg tcagctaaag atgtcagtgt agtttctttt gaaagaatat
     4921 ataccattga agtcgaggta tactgcgggg taatagcaaa cttgctgaac agaccgaagg
     4981 cgccccccat gatcacgcct gcgccaacga tccagagaat tctcttcttt aacgcgcgga
     5041 acagctggag aagatcgatc tctacttcgt catcattcat gttgattgtc ttgttatcca
     5101 tacgtacttt ttccttttct tcctattatt atatagtttc tttttttatg acgcgcaacg
     5161 cgttctggta agtaatgcac cagatctgtt gtgcatcaat atgaccgtca agccatttga
     5221 gtgcctctgt gatttgaggc ggtctgtaat ctgacctgtg catgtctgtc cccaggaaat
     5281 ggatcactcc tgattttacc tgttccctgc accaccgtac ctccggtgag aaccagtgtc
     5341 cctggatact gtcgtaattc atctgaagtt tacatccaat atcaaggagc tctgaagttc
     5401 ctttttttcg caggcaaccg taacgctcca tatgagcaag aataggcgtg tacccgttag
     5461 agaccagttt gcggatcccc tgaaaaagaa tttcataaga aacagaaggc ataaattcta
     5521 ccagaacata ttggctgtct gccatggtat atgccttccc ctcattcagg cgctgtgcaa
     5581 gctcatcgtg ataaaatgtt tcctggcccg gaaagatcct gaacttagga taggttttcc
     5641 gaatttcctg ctgaagaagc tttgctgagt catttaatcc tttcagcaca tggcgtctgg
     5701 aatagtgagg tgtagcgatc acacctacaa tcccctggtc ggccgcccgt ttcagcattt
     5761 caacggattc ttccattgtc cttgatccat catccacacc cggtatcaca tgtgcatgaa
     5821 catctattat tcgctgccga actttcattt ttattagtac ctttcttccg gaatattctc
     5881 aatgggtttt aatgcagaaa taagaagtgt caatttttaa gaataccccc ccccagctaa
     5941 atactatata gtttccgctt cggcacgccg ggactgccta aaaggggatt ttttctatga
     6001 atgattttac atcatattct ttataaaaga aaggtcattt ttgttttttt atgaaaaata
     6061 aggccaaagt tattgtttgt aacctttgcc ttagtgaatt acttcctatt atatagtgtt
     6121 acactgcgtt atttcagtta caaagccaat tgatttgcca ggatcacact ggctgccagg
     6181 ctgacaaacg ctgctaaaag tccgcctgca agtgtataac gggtacggct gatgcctgca
     6241 gaaccgaaat agacactcat acagtaaagc acggtttccg tacttcccat aaggatagat
     6301 gccataagcc ccacctggga gtcgggacca taatttttaa acagatccag caccaggcct
     6361 actgcggcag agttggaaac gagacgtata aaagcaatgg ggataatggg gccggggaga
     6421 ccgatggaag tagccggaac ttctaaaata tgggttaaaa attctaaaaa tcccgatgca
     6481 cgaaggacgc ctaccgctgt cattaatcca actaatgaag gaaggacctg gacaacggtc
     6541 tgcatcccct cttttgcgcc atataaaaaa tcatcaaata ccggtctttt tccaagtatg
     6601 gcgaaaccaa caatataaaa aatgatcagt ggaaccataa aatctgataa aaataagaga
     6661 aagcccataa aaaatgcctg cccccacgca tgttttgttt ttacagcata cgctggaaca
     6721 ggttttatta tgtaacaaat atatcccccg cgcaagcgca ggggatataa gtaggaagta
     6781 tgcattttgc gaatatacga tccagattat gcctcagtaa ataatggagt ggagtaatat
     6841 ctgtcgccac tgtccggaag aagagctaca atggtctttc cttcgttttc aggacgtttt
     6901 gcaagttgga tcgctgcgta taatgctgca ccggaagaga taccggttaa gataccttct
     6961 gtttttgcaa gaagtttgga tgcttcaaac ggatgctcat tgtcgatggg cataacctca
     7021 tcgtaaatct tggtgtctaa tacttttggt acgaaaccgg cgccaattcc ctggatcttg
     7081 tgggcaccgc cgtgtccttc agacagtaca ggagaagttg caggctctac tgctactacc
     7141 tttacatttg ggttctgctc ttttaagtat gtgccaacgc cggtaatggt accgccggta
     7201 cctacgcctg ctacgaagaa gtctacctga ccctctgcat ctctccagat ttcaggacct
     7261 gtggtctctt tgtggatctt cgggtttaca gggtttacaa actggcctgg gataaagctg
     7321 tccgggattt cttttgccag ctcatctgct tttgcgatag ctccgctcat tcccttatca
     7381 ccaggtgtga gaacgatttc agctccatag gcctttaaga tgttgcggcg ctctacgctc
     7441 atggtctcag gcatggtcag gatcacacgg tagcccttgg atgctgctac agatgcaagt
     7501 ccgatacctg tatttccgga agttggttca atgataacag aacctggctt taaaacacct
     7561 cttgcttctg catcttcgat catcgctttt cctacacggt cttttacact tcctgccggg
     7621 ttaaagtatt ccagcttcag taaaatcctt gcctttaatc cttctttttt ctccagattt
     7681 accggttcta aaagtggtgt gtttcctact aatcctgctg ctcccttaac gattcctgcc
     7741 ataataagct cctttccatg cattgtatga taccagatcc ttcacctttt gatcctggtc
     7801 ccatatgcct atctgtttac tgggttttga tttatgggta ttataaaacc tataaaccca
     7861 cctgtcaata ggtttatggg ttttttgtat tgctg
//
LOCUS       DBMDUA010000134         7868 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4098_length_7868_cov_2.490923, whole genome shotgun sequence.
ACCESSION   DBMDUA010000134 DBMDUA010000000
VERSION     DBMDUA010000134.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4098_length_7868_cov_2.490923"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /gene="alaS"
                     /locus_tag="ACZPCL_09905"
     CDS             complement(<1..1454)
                     /gene="alaS"
                     /locus_tag="ACZPCL_09905"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR1162816.1"
                     /translation="MKQPQYRGVNELRRMFLEFFESKGHLAMKSFSLVPHNDNSLLLI
                     NSGMAPLKPYFTGQEIPPRRRVTTCQKCIRTGDIENIGKTARHGTFFEMLGNFSFGDY
                     FKTEAIHWSWEFLTEVVGLDPDRLYPSIYQDDDEAFDIWNKEIGIAPERIFRFGKEDN
                     FWEHGSGPCGPCSEIYYDRGEKYGCGKPGCTVGCECDRYIEVWNNVFSQFDNDGHGHY
                     SELKQKNIDTGMGLERLAVVVQDVDSLFTVDTNKALLDRVCALSNKEYQKDHETDVSL
                     RIVTDHIKSCTFMISDGIMPSNEGRGYVLRRLLRRAARHGRKLGIEGKFLAELSKTVI
                     ELSKDGYPELEEKQSMIFKVLTEEEDKFNKTIDQGLAILAEMEDAMAAAGEKTLSGEN
                     AFKLYDTYGFPVDLTKEILEEKGCTVDEEGFAAAMKEQKDKARKARKTTNYMGADVTV
                     YQSIDAAITSEFVGYDKLDVDSKISVLTTEDEIV"
     gene            complement(1679..2419)
                     /locus_tag="ACZPCL_09910"
     CDS             complement(1679..2419)
                     /locus_tag="ACZPCL_09910"
                     /inference="COORDINATES: protein motif:HMM:NF018684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HIR1162817.1"
                     /translation="MKDRRIWVIIGCILVVGIAITSYTSSVVKKVPQEQKIQVLSETT
                     AETAVDSDSAPLQAVPREAQAKMKAGDPEQTEAAGAEIRSEKETEARTAEETEESNSS
                     EDQVQVQAADPPDEGLEPVLHDAEPEQESYTDRLRNLDSQIQKMQEQETDSNVYSAKT
                     SAETQLKMWDGELSTVYNALLEALPQEEAQSLANEERDWLKKRESDAADSSGKSGNSL
                     SYASSMVNQTRERAYELAKRCDALAEEN"
     gene            complement(2562..3044)
                     /gene="rnhA"
                     /locus_tag="ACZPCL_09915"
     CDS             complement(2562..3044)
                     /gene="rnhA"
                     /locus_tag="ACZPCL_09915"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HIR1162818.1"
                     /translation="MTEVKIYTDGSARGNPDGPGGYGVVMHYVDKNGQLHEKTLSAGY
                     KKTTNNRMELMAAIAGLEALNRPCKIELYSDSKYLTDAFNKNWISGWIAKGWNRGKSG
                     PVKNIDLWKRLLKAKEQHQVTFIWVKGHAGHPQNERCDQLATTAADGNNLIEDEGLSL
                     "
     gene            complement(3066..4673)
                     /locus_tag="ACZPCL_09920"
     CDS             complement(3066..4673)
                     /locus_tag="ACZPCL_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR1162819.1"
                     /translation="MLMKLTFIGADHEVTGSCHFLEVGDTKVLIDCGMEQGNNPYQNA
                     ELPVSYSDIDYVFLTHAHIDHAGMLPWIYARGFKGQIITTYATADLCGIMLRDSAHIQ
                     EMEAEWRNRKARRAGKTEVEALYTMQDAEGVLGHFEGVAYGQKFKLNENITLRFTDVG
                     HLLGSASIEIWATEGDEQRKIVFSGDIGNKNKPLIRDPQYISDGDYVVMESTYGNRYH
                     KKGEDHVVGLARIIQQTLDRQGNVVIPAFAVGRTQELLYMIRHIKEEKLVTGHDGFQV
                     YVDSPLAVEATHVFKQNMVECYDEETKALVERGINPIDFPGLKLSVTSEESKNINFDM
                     TPKVIISAAGMCDAGRIRHHLKHNLWREECSVVFAGYQAEGTLGRLLQDGAPAVKIFG
                     EEIDVKAHIETLDGISGHADRDGLLTWAAAFEQKPKYVFVVHGNDESCESLTDALNTQ
                     LGLTAKAPYSGSEFDLIKGVWIKETEPVPVEAETQATRKATGVYTRLVDAGQMLMDVI
                     ARNQGGANQDLTRFTEQILALCEKWNR"
     gene            complement(4901..6382)
                     /gene="spoIVA"
                     /locus_tag="ACZPCL_09925"
     CDS             complement(4901..6382)
                     /gene="spoIVA"
                     /locus_tag="ACZPCL_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HIR1162820.1"
                     /translation="MDNFNVYKDIQARTKGEIYIGVVGPVRTGKSTFIKRFMELAVLP
                     AMEDESLKAVSTDELPQSAGGKTVMTTEPKFIPKEAVQITLGDGIQAKVRLIDCVGYM
                     VDGAAGHVENGEERMVKTPWSEKEIPFTQAAQIGTRKVIGDHSTIGIVVTTDGSVGEL
                     KRSAYIDAEKQTVDELKKLGKPFILLLNSTKPYSDETLKLAEQLAADYGVTVLPVSCE
                     QLKKEDIFHILESVLKEFPVTQLDFHIPKWLEVLPATHWLKTQVIDMARELLKKVSHM
                     KDAASQIKTFGGSSGPVEKITIEKMEMADGTVSLQVQMDDSYYYQILSDYVGLPIEGE
                     YQLMQTLSTLAGMQKEYDKVKEALAQARLKGYGVMMPQKDEILLDEPEVIRHGNKYGV
                     KMKAQAPSVNLIRAQIETEIAPIVGSEQQAKDLIAYIKSSSKDSEEGIWNANIFGKSI
                     EQIVEDGIQAKISQMTDECQQKLQDTLQKIINDSNGGMICIII"
     gene            complement(6433..7143)
                     /locus_tag="ACZPCL_09930"
     CDS             complement(6433..7143)
                     /locus_tag="ACZPCL_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1162821.1"
                     /translation="MAMLEVKDLEVYYGVIQALKGISFDVDQGDIVALIGANGAGKTT
                     TLHTITGLLNAKAGKIIYEGKDITHIPGYKLVSMGIAHVPEGRRVFATLSVLQNLMMG
                     AYTRRDKNEIEETLKMIYKRFPRLEERKNQLAGTLSGGEQQMLAMGRALMSHPKVMVL
                     DEPSMGLSPIYVNEIFDIIQQINKDGTTVLLVEQNAKKALSIANKAYVLETGTIALSG
                     DAKELMNNDRVKKAYLSE"
     gene            complement(7158..>7868)
                     /locus_tag="ACZPCL_09935"
     CDS             complement(7158..>7868)
                     /locus_tag="ACZPCL_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1162822.1"
                     /translation="KAVDNFHINIEKGQLYGLIGPNGAGKTTIFNLLTGVYKPNAGSI
                     VLDDVNITGKSTIEINQAGIARTFQNIRLFKDMSVLDNVKAGLHNHHKYSTVEGIFRL
                     PRYFKIEKEMDEEAMNLLKVFDLDKECDFKASNLPYGKQRKLEIARALATEPKLLLLD
                     EPAAGMNPNETAELMDTIRFVRDNFNMTVLLIEHDMKLVSGICEKLTVLNFGQVLREG
                     ATSDVLHDPEVIKAYLGE"
BASE COUNT         1861 a         2003 c         1576 g         2428 t
ORIGIN      
        1 tctacgatct catcttctgt agtcagaaca gagatcttgg agtctacatc taatttatca
       61 tatcctacaa actcagaggt gatcgcagcg tcaatagact gatatacagt tacatctgcg
      121 cccatgtagt tggtagtctt acgagcctta cgagccttgt ccttctgctc cttcatagct
      181 gctgcgaagc cttcttcatc aacggtgcag cccttttctt ccaggatctc cttggtcagg
      241 tctacaggga aaccataagt atcatacagc ttaaatgcat tctcaccgga aagagtcttc
      301 tcaccggcag ctgccatagc atcttccatt tcagctaaga tagcaagacc ctggtcaatg
      361 gtcttgttga acttatcttc ttcttccgtc agtaccttaa agatcatgga ctgtttctct
      421 tccagttccg gatatccgtc cttggaaagt tcgataacag tcttggaaag ctctgcaagg
      481 aacttgcctt cgatgccaag ctttcttccg tggcgtgccg cacgacgcag cagacggcgc
      541 agaacatagc ctcttccttc attagaaggc atgataccgt cagagatcat gaaggtacag
      601 gacttgatat ggtctgtaac aatacgaaga gaaacatcgg tctcatggtc tttctggtat
      661 tccttgttag aaagagcaca tactctgtct aacagagcct tgttggtatc tacagtaaac
      721 agggagtcta catcctggac aacaactgcc agacgctcca gccccatacc tgtatcaatg
      781 ttcttctgct tcagttcgga ataatggcca tgaccgtcat tatcaaactg gctgaatacg
      841 ttgttccata cttcgatgta acggtcgcac tcgcagccta cggtacatcc tggcttaccg
      901 cagccgtact tctcacctct gtcgtagtag atctcagaac atgggccgca aggacctgaa
      961 ccatgctccc agaagttgtc ttccttacca aaacggaaaa ttctctctgg tgcaatacca
     1021 atctccttgt tccagatatc aaatgcttca tcatcatcct gataaataga aggatacaga
     1081 cgatcaggat ctaagccaac tacttctgtt aagaattccc aggaccagtg gatcgcttct
     1141 gtcttgaagt aatcaccgaa agagaagttt cctaacattt caaagaaagt accgtgacgt
     1201 gcagtctttc caatattttc aatatcacct gtacggatac atttctggca ggtagtcact
     1261 cttcttcttg gcgggatctc ctgtccggta aagtaaggct ttaaaggtgc cataccggag
     1321 ttgatcagca gaaggctgtt atcattatgc ggtacaagtg aaaagctctt catagccaga
     1381 tgacccttgc tctcaaagaa ctcaaggaac atacggcgca gctcgttcac tccgcggtac
     1441 tgtggttgtt tcactttttt cttcctccat tatataaatt tgatttaggg tttttcagta
     1501 aatatttcag gacagcaaaa atccgggcag aacagacttc gcctgtgaat gccctcctaa
     1561 aacaaaccat gtatataaac tcataatcgg aattttacca tataagcgct gaattagcaa
     1621 gagaaaatga ctccggaact atgtccagag tcattttttc attgtaacag atctctattt
     1681 aattttcttc tgccagtgca tcacaccgtt ttgccagctc ataggccctc tctctggtct
     1741 gattgaccat gcttgaggca taacttaaac tgtttccgct ttttccggag ctgtcagctg
     1801 cgtctgactc tctttttttc agccagtctc tttcctcatt tgcaaggctt tgggcctctt
     1861 cctggggaag ggcttctaat aatgcattgt atacagtgct taattcccca tcccacattt
     1921 tcaactgggt ttctgcagaa gttttagcgg aataaacatt ggagtctgtt tcctgttcct
     1981 gcattttctg gatctggctg tctaagtttc tcaggcggtc tgtatagctt tcctgttccg
     2041 gttctgcatc atgtaaaacc ggctccaaac cttcatccgg aggatctgcc gcctggacct
     2101 gcacctgatc ttctgaggaa ttgctttctt ctgtttcctc tgccgtcctg gcttctgttt
     2161 ctttctcaga acgtatctcg gcacccgctg cctctgtctg ttccggatca cctgctttca
     2221 tttttgcctg tgcttcccgg gggactgcct gcaaaggcgc agagtcacta tccactgctg
     2281 tttcagccgt ggtctcagaa agaacctgta ttttctgttc ctgtgggact ttttttacaa
     2341 cggaacttgt atagctagta atggcgatcc ctactaccag gatacaaccg atgatgaccc
     2401 atatccttct gtctttcatt ctttcacatc cctgataaag agtaatgttt tccagtcata
     2461 cagtaccaca aaatcaggtg cttgtgaata caaattcgaa aggagtaata ttttgtaaga
     2521 tttgcgtaaa aattggggct gtgttgaaac agccccatgg atcataagga taagccctca
     2581 tcttctatta aattatttcc gtctgccgct gtagtagcca gctggtcaca ccgctcattc
     2641 tgtggatgtc cggcatggcc ttttacccag atgaatgtga cctgatgctg ctcttttgcc
     2701 tttaaaaggc gcttccacag gtcaatgttt ttcaccggtc cgcttttgcc ccggttccag
     2761 cctttggcga tccagccgct gatccagttt ttgttaaagg catctgtaag atatttcgag
     2821 tctgaataca gctcgatctt acaggggcga tttaaagctt ccaagcctgc tatggctgcc
     2881 attaactcca tacggttatt ggtggtcttt ttgtaacctg cagataaggt cttttcatgt
     2941 aactggccat ttttatccac ataatgcatg accactccat aacctcccgg tccgtctgga
     3001 tttcctcttg ctgatccatc tgtgtagatc tttacttctg tcatttttct ctgtactcct
     3061 gttgtttatc ggttccattt ttcgcaaaga gctaatatct gctctgtaaa acgggtcaga
     3121 tcctgatttg ccccgccctg gttgcgtgcg atcacatcca taagcatctg tcctgcatcc
     3181 accagtctgg tgtaaacgcc tgttgccttt ctggttgcct gagtttctgc ctctaccgga
     3241 acaggctcgg tctccttgat ccatacaccc tttatcaggt caaattccga accggaataa
     3301 ggtgcttttg ctgtaaggcc aagctgggta tttaaagcat ccgtaagaga ctcacaggac
     3361 tcgtcatttc catgtactac aaatacatat ttgggctttt gttcaaaggc tgcagcccag
     3421 gtaagaaggc cgtctctgtc tgcatggccg ctgataccat ccaaagtctc aatatgcgcc
     3481 ttcacatcga tctcttcacc aaagatcttt acagcagggg caccatcctg aagcagacgg
     3541 cctaaggttc cttctgcctg gtatccggca aataccacac tgcattcttc cctccacaga
     3601 ttatgtttta agtggtggcg gatacggccg gcatcacaca tacctgctgc ggaaatgatc
     3661 accttcgggg tcatatcaaa gttgatattt tttgactctt cactggtaac ggaaagcttt
     3721 aatcccggga aatcaattgg gttgataccc cgttctacca acgcctttgt ctcctcgtca
     3781 tagcattcca ccatattctg cttaaataca tgggtagctt ctactgccaa cgggctatcc
     3841 acatatacct ggaagccatc gtgaccggta accagctttt cttccttaat atggcggatc
     3901 atataaagaa gttcctgggt cctgcctact gcaaaggccg ggatcactac attgccctgg
     3961 cggtctaagg tctgctggat gatacgggcc agacctacta catggtcttc gccctttttg
     4021 tgatagcggt ttccataggt ggactccatc actacataat cgccgtcaga aatatactgg
     4081 ggatcccgga tcagcggttt attcttgttt ccgatatcac cggaaaagac gatctttcgc
     4141 tgttcatccc cttctgtcgc ccagatctca atagaggctg aaccaagaag atggcctaca
     4201 tccgtaaaac gcagggtgat gttttcgttt aatttaaact tctgtccata agccacacct
     4261 tcaaaatggc ccagaactcc ttctgcatcc tgcatggtat aaagggcttc tacctctgtt
     4321 ttgccggctc tgcgtgcttt acgattgcgc cactctgcct ccatctcctg aatgtgggca
     4381 ctgtcccgaa gcataattcc acacaggtct gcagtcgcat aggtggttat gatctgcccc
     4441 ttaaatcctc tggcatagat ccagggaagc atgcctgcat ggtcaatgtg ggcgtgggtc
     4501 agaaatacgt agtcaatgtc gctgtaactg accggaagtt ccgcattctg gtaaggattg
     4561 ttcccctgct ccataccaca gtcaataagg acttttgtat ctcctacttc cagaaaatga
     4621 cagctgcctg taacttcatg gtctgctccg ataaaggtca gtttcataag cattctcccc
     4681 tctgttgccc ctttttattg ttattataag ggtctcgttt ttgctgactc tagtatagca
     4741 catttgcatg tttatttgca aactttttca gttattttgc ataaattaga caagttttct
     4801 gataatgtcg cttactacgc atatgccacc gggtcccaag ctcgaaaatc ctcttgcaag
     4861 caagcttgca tcggattttt gagttttgtc ccctggtgtg tcatatgata atacagatca
     4921 tccccccatt actgtcatta atgatcttct gcagtgtatc ctgcaatttc tgctggcact
     4981 catcggtcat ctgggagatc ttggcctgga tgccgtcttc tacgatctgt tcaatggatt
     5041 tgccgaagat attggcattc cagatgcctt cttcactgtc tttggaactg gattttatgt
     5101 atgcgatcag gtcctttgcc tgctgctcgc ttcccacaat aggagcgatc tcggtctcga
     5161 tctgggcccg gatcaggttt acagagggcg cctgggcctt cattttcacg ccgtacttat
     5221 ttccatgacg tatcacttcc ggttcatcta ataatatctc atctttctga ggcatcatga
     5281 ccccatatcc ctttaatcta gcctgggcca gggcctcttt tactttatca tactctttct
     5341 gcatacctgc taaagtgctt aaagtctgca tcagctggta ctcaccttct ataggcagtc
     5401 ctacatagtc gcttaatatc tgataataat agctgtcatc catctgtacc tgtaaggata
     5461 cggtaccgtc tgccatttcc attttttcga tcgtgatctt ttccacaggt cctgatgagc
     5521 cgccaaatgt ctttatctga gaagctgcat ctttcatgtg gctgactttc tttaaaagtt
     5581 cccttgccat atcgatcacc tgggttttaa gccagtgggt agccggaagg acttccagcc
     5641 atttgggaat atgaaaatcc agttgcgtaa caggaaattc ttttaaaaca ctttccagaa
     5701 tatggaaaat atcttctttt ttcagctgct cgcagctgac gggaagaact gtcaccccat
     5761 aatcagctgc cagctgttct gccagcttta aggtttcatc tgaatagggt ttcgtagaat
     5821 ttaaaagaag gataaatggc ttccccagct ttttcaattc atctacagtc tgtttttccg
     5881 catctatata ggcagagcgc tttaattccc caacagagcc gtccgtagtc actacaatgc
     5941 caatggtaga atgatcccct atgactttcc tggtgccgat ctgggccgcc tgggtaaagg
     6001 ggatttcttt ttcagaccag ggcgttttca ccatccgctc ttccccattt tccacatggc
     6061 ctgcagcccc gtctaccata tagcctacac agtcgatgag gcgcactttc gcctggatcc
     6121 cgtctcccaa agtgatctgc accgcctctt tggggataaa tttcggttcc gttgtcatca
     6181 ccgtttttcc ccctgcgctc tggggaagtt catcggtact tactgccttt agactttcat
     6241 cttccatagc cggaagcaca gccagctcca taaaacgctt gataaaggta gactttcctg
     6301 tacgcaccgg tcccaccaca ccgatgtaga tctctccctt tgttctggcc tgaatatcct
     6361 tgtatacatt aaaattgtcc ataaaaatac ccccttgcac gtttatataa catatgcaag
     6421 agggtatgat ctttattcgc ttaaataagc cttctttaca cggtcgttat tcatcagttc
     6481 ttttgcatcg ccggaaaggg caatggttcc tgtttccaga acatatgcct tgttagcaat
     6541 agaaagtgct ttctttgcgt tctgctctac taaaaggacc gtagtgccgt ctttgttgat
     6601 ctgctggata atatcaaaga tctcatttac ataaataggt gaaagtccca tagacggctc
     6661 atctaatacc attaccttcg gatggctcat aagagcacgg cccatagcaa gcatctgctg
     6721 ctcgccgccg gataaggttc ctgctaactg attctttctc tcttccagac gtgggaaacg
     6781 tttatagatc atctttaatg tttcttcgat ctcattttta tccctgcggg tataagcgcc
     6841 catcatcagg ttctgtaaaa cagaaagggt cgcaaataca cggcgtcctt ccggaacatg
     6901 ggcaattccc atacttacca gcttatagcc cggaatgtgg gtaatgtctt ttccctcgta
     6961 aatgatcttt cctgcttttg cgtttaacag gccggtaatg gtatgcaaag tggtggtctt
     7021 tcctgcacca ttggcaccga tcagcgctac aatgtctccc tggtctacat caaaggagat
     7081 tccctttaaa gcctggatca caccatagta tacttccagg tcttttactt caagcattgc
     7141 catactatct ctcctcctta ttcacccaga tatgccttga taacttccgg atcatgcagt
     7201 acatcactgg tagcgccttc tcttaagacc tgtccgaagt ttaaaacggt cagcttctcg
     7261 cagataccgc ttaccagctt catatcatgc tcaataagaa gtacagtcat gttaaaattg
     7321 tcacgtacaa aacggatggt atccatcagt tctgctgtct cgttagggtt catgcccgct
     7381 gccggctcat ctaacagaag gagctttggc tctgttgcca gggctcttgc gatctctaac
     7441 tttctctgct taccataagg taagttggaa gccttaaaat cacattcctt atccaggtca
     7501 aataccttta acaggttcat ggcctcttcg tccatctctt tttcaatctt aaaatatctc
     7561 ggaagacgga aaataccttc tactgtagaa tatttatgat gattgtgaag tcctgccttt
     7621 acattatcaa gaacggacat atccttgaaa agacggatat tctggaaggt tcttgcaata
     7681 ccggcctgat taatctcaat ggtacttttt ccggtaatat ttacatcgtc taaaacgata
     7741 cttcctgcat ttggcttata tacacctgta agaaggttga aaatggtggt ctttccggca
     7801 ccgtttggtc cgatcagtcc atacagctgt cccttttcaa tgttgatatg gaaattatca
     7861 actgcctt
//
LOCUS       DBMDUA010000135         7743 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4164_length_7743_cov_2.320858, whole genome shotgun sequence.
ACCESSION   DBMDUA010000135 DBMDUA010000000
VERSION     DBMDUA010000135.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4164_length_7743_cov_2.320858"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="ACZPCL_09940"
     CDS             <1..940
                     /locus_tag="ACZPCL_09940"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR1162823.1"
                     /translation="QELTLAYANESRQVINQYMPGDETSFTIIAFPKPEIGPDFEDIF
                     RETIAINTLDYEKYQKIQQKLIDALDKADYVEITGRDGNETSMKVQLHTLTDPEKQTN
                     FENCVSDVNIPLGEVFTSPVLTGTEGLLHVKEVYVEDYLFKDLRMVFKDGKVTEFGCG
                     NFPESKEQGKDLVKQVIMRGHSWLPLGEFAIGTNTTAYAMAKKYQIGSKLPILIAEKT
                     GPHFAVGDTCYSFAEDSPMYNPDGKEVIARDNEISLLRKEDMSKAYFSCHTDITVPYC
                     ELGDIVAVGKDGSRITLIRQGRFALEGTEGLNEALD"
     gene            1119..2864
                     /locus_tag="ACZPCL_09945"
     CDS             1119..2864
                     /locus_tag="ACZPCL_09945"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1162824.1"
                     /translation="MLKVLSKCIREYKQASLKAPLMVSLEVVMECIIPFIIAQLVNRM
                     KAGAGFSEIAMYGLVLVVMATLSLIFGITAGNACATASCGFAKNVRKDLFYKIQDFSF
                     ENIERFSSSSLVTRLTTDVTNVQMAYMMIVRIAIRAPLMLVFAFVMAFIMGGRMACIF
                     IIVVPILGIGLFSIIRLVMPLFRAVFNKYDALNNSVQENIKGIRVVKSYVREEYEKEK
                     FDRAAEDVCGDFTRAEKILAFNNPLMQFCLYTVTIFVLSFGSYTIITSRGLDLDVGQF
                     SALLTYSFQILSSLMMVSMVFVMITLASESAKRIVEVLEEKSSLVNPENPVYEVKDGS
                     VDFENVSFKYSKKAERNALENINLHINSGETIGIIGGTGSSKSTLIQLISRLYDTTEG
                     VVKVGGRDVREYDLKTLRNQVAVVLQKNILFSGTIEENLRWGDKNATPEEIAHACKLA
                     QADEFVLRFPDKYKSYIEQGGANVSGGQKQRLCIARALLKKPKILILDDSTSAVDTKT
                     DALIRSGFKEFIPETTKIIIAQRISSVQEADHIIVMDGGKIQAIGNHEELMKTSPIYK
                     EVYDSQNKGGGQDEE"
     gene            2854..4761
                     /locus_tag="ACZPCL_09950"
     CDS             2854..4761
                     /locus_tag="ACZPCL_09950"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009536342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1162825.1"
                     /translation="MKNNGKGMAKAGKKTFLRLIRTIFGFFPVLMPLVLIGILFNAIV
                     GSIPAVFMQKVIAVVEQNWQSGDWNGCKDEILRYTGILLTLYGLALAASIFYNRKMAE
                     ITQGTLKKLREKMFNSMQDFPVKYFDTHNHGDIMSYYTNDIDTLRQLISQSIPQFLNS
                     AVMVTTVFCVMLYYSIWMALVVVAGVVLMLHIIKKVGGSSAKFFIQQQKAIGKTEGFV
                     EEMMNGQKVIKVFCHEEESEKAFDAINDELFHVSETANKYANTLGPILNNIGNLLYVA
                     VALAGSLFLMFKVPNLSISRLPFSISIVVPFLNMTKQFAGNINQVSQQINAVVMGMAG
                     AERIFGLLDEKPEEDEGYVTLVNAVENPDGTLSEHKERTGVWAWKHPHSDGTVTYTRL
                     TGDVRFENVDFGYDPGKTVLHNITLYAEPGQKVAFVGATGAGKTTITNLINRFYDISD
                     GTIRYDGINIRKIKKSDLRHSLGIVLQDTNLFTGTVMENIRYSNPDATDEECIGAAKL
                     AGAHDFITRLPEGYETVITGNGENLSQGQRQLISIARAAAADPPVMILDEATSSIDTR
                     TEAIVQRGMDALMQGRTVFVIAHRLSTVRNSDVIMVLEQGHIIERGSHDKLIEEKGKY
                     YQLYTGAFELE"
     gene            4988..5800
                     /locus_tag="ACZPCL_09955"
     CDS             4988..5800
                     /locus_tag="ACZPCL_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIR1162826.1"
                     /translation="MVERKAFLKAYNIDEDDFNAADISWEELAAIYDDYLSKEEKLRG
                     IGKDFVNDYLYDIERAGIHSYRYRTKNPGHLLEKIIRKRKEDPEKFKDLNRSNYYKYM
                     TDLIGIRVFFLYREDWIYFHKYITTVFENDPSIYVKDRGRDFDEDPSHCYIAERPKVY
                     RRNGDTRIYDEKLIDIKSDGIYRSLHYIIKYKGYYVEIQGRTLFEEGWSEIDHDIVYP
                     YYKDDEMLKDFSTLLNRLSGMADEMSSYFRRMRSVREEQGLLSHHSGEGKKE"
     gene            complement(5927..7327)
                     /gene="mgtE"
                     /locus_tag="ACZPCL_09960"
     CDS             complement(5927..7327)
                     /gene="mgtE"
                     /locus_tag="ACZPCL_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR1162827.1"
                     /translation="MENKVQVQDHSQEILSVIRSNASPGILRNKLEDYHENDLADIFP
                     ELTLAERRKICRILDTDMLSDIFEHIDQKQAAEYLDEMDVKKAAAILSAMETDAVVDV
                     LKMTSKEKRSLLTELLDDDAKKDIAVIAAFDEDEIGSWMTTNCIVIRENLTVKQAMSS
                     LVEQAAKNDNISTIFMVTEDQTFYGAMDLKDLIIARQDSALEDLIVTSYPYVYGHELI
                     DDCIEKLKDYSEDSIPVLNNNNKLLGVITSQSIVDLVDDEMGEDYAKFAGLTAEEDLK
                     EPLKESLKKRLPWLLVLLALGMVVSSVVGLFEQVVSQLTLIMCFQSLILDMAGNVGTQ
                     SLAVTIRVLMDESLTGKQKLELVWKEMRIAFSNGLILGVLSFTAIGLYIVVAKGKPFA
                     FAYAVSGCIGAALLLAMVISGAVGTCIPLFFKKINVDPAVASGPLITTVNDLVAVVSY
                     YGLSWILLINVLHLAG"
BASE COUNT         2257 a         1547 c         1802 g         2137 t
ORIGIN      
        1 gcaggagctg acactagctt atgcaaatga gtcacgccag gtcatcaacc agtatatgcc
       61 gggagatgaa accagcttta ctattattgc tttcccgaag ccggagatcg gaccggattt
      121 tgaggacatt ttccgtgaga ccattgccat caacacactg gactacgaaa aataccagaa
      181 gatccagcag aagctgatcg atgccttaga caaggcggac tatgtggaga tcacaggacg
      241 ggatggaaat gaaacttcca tgaaggtgca gcttcatacg ctgaccgacc ctgaaaagca
      301 gactaatttt gaaaactgcg tatccgatgt aaatattccg ttgggagaag tatttacctc
      361 tcctgtgctt acaggcacag aaggccttct tcatgtaaaa gaggtttatg tggaagatta
      421 tctctttaaa gacctgcgta tggtatttaa agacggaaaa gtaaccgagt ttggctgcgg
      481 aaatttccca gagtccaaag agcagggaaa agacctggta aaacaggtga tcatgcgcgg
      541 ccacagctgg cttccgctgg gcgagtttgc cattggaacc aacaccacag cttatgcgat
      601 ggcaaagaaa taccagatcg gcagcaagct gcctatcctt attgctgaga aaacaggtcc
      661 acattttgca gtaggagata cctgctacag ttttgcagaa gactcaccta tgtacaaccc
      721 ggacggcaaa gaagtgatcg ccagagacaa tgagatctcc cttttaagaa aagaggatat
      781 gtcaaaggca tatttcagct gccatacaga tattacggtt ccttattgcg aactgggaga
      841 tattgtggct gtgggaaaag acggcagccg gatcacactg atcagacagg gaagatttgc
      901 cttagaggga acagaaggtt taaatgaggc tctggattaa tatttatttc acaaaaagtt
      961 cattatttta ctcttgtaaa gaagagtaga tttaggttat gattaaaagt agcctaaaat
     1021 gcacatgttt cagtataagt actgaagaaa gcattcagga cgttcgcact gcgggcgtcc
     1081 tgttcttatt gtttacagaa aggagtattt tacctactat gctgaaagtt ttatcaaaat
     1141 gcatccgtga gtataagcag gcatccctga aagcaccttt gatggtatcc ctggaagtgg
     1201 taatggagtg tatcattcca tttatcattg cacagctggt aaaccggatg aaggcaggtg
     1261 ccggtttcag tgagatcgcc atgtatggac tggtgttggt cgttatggca acactttctc
     1321 ttatattcgg catcactgca ggtaatgcct gcgccactgc ttcctgcggg tttgcaaaga
     1381 acgtgagaaa agacctgttt tataagatcc aggacttttc ctttgaaaac attgaacggt
     1441 tttcatcttc atctttagtt acccgcctga ctacagatgt gactaatgta cagatggctt
     1501 atatgatgat cgtccgtatt gccatacgtg caccattaat gcttgttttt gcctttgtca
     1561 tggcgtttat catgggaggc agaatggcat gtatttttat cattgtagtg cccatactgg
     1621 gaattggtct tttttccatt atccgtctgg tcatgccgct gttccgcgct gtatttaaca
     1681 aatatgatgc tttaaataat tctgttcagg aaaatattaa gggcatccgt gttgtgaaat
     1741 cttatgtgag ggaggagtat gaaaaagaga agtttgaccg tgcagcagaa gatgtatgtg
     1801 gtgattttac ccgtgcagaa aagatccttg catttaacaa tccgctgatg cagttctgcc
     1861 tgtatacagt tacgatcttt gtgctgtctt ttggttccta taccattatc accagccggg
     1921 gactggatct ggatgtagga cagttttccg ctcttttaac ctatagtttc cagatcttaa
     1981 gcagtcttat gatggtgtct atggtctttg ttatgatcac actggcttct gagtctgcaa
     2041 agcgaatcgt agaagttctg gaagaaaaga gcagtctggt aaatccggaa aatccggttt
     2101 acgaggtaaa ggacggttct gttgattttg aaaatgtaag ctttaaatat tctaaaaagg
     2161 cagagagaaa cgctctggag aacattaacc ttcatattaa ttccggtgag actattggta
     2221 ttatcggtgg aaccggttct tccaagtcta ctttgatcca gctgatctcc cgtctgtatg
     2281 ataccacaga aggtgtggta aaggtggggg gaagagatgt aagggaatat gaccttaaga
     2341 cactgcgaaa ccaggttgct gttgttttac agaagaatat cctgttttca ggtactattg
     2401 aagaaaacct gcgctggggt gataaaaacg ccactccaga ggaaatagcc catgcctgca
     2461 aactggccca ggcagatgaa tttgttctcc gtttcccgga caagtacaag tcttatattg
     2521 agcagggcgg tgccaatgtt tccggtggtc agaagcagag actttgtatt gcaagagcgc
     2581 tgcttaagaa gccgaagatc ctgatcttag atgactctac cagtgcagta gatacaaaga
     2641 cagatgctct gatccgcagc ggatttaagg aatttatccc ggagaccacc aagatcatta
     2701 tcgcacagcg tatttcatct gttcaggaag ccgatcatat tattgtaatg gacggcggta
     2761 agatccaggc aattggaaat cacgaagaac tgatgaagac cagcccgatc tacaaagaag
     2821 tatacgactc acagaacaaa ggaggcggtc aggatgaaga ataatggaaa aggcatggca
     2881 aaggctggaa aaaagacgtt tttgcgcctg atccgcacca ttttcggatt tttcccggtg
     2941 ctgatgcctc tggtactcat aggtatttta tttaatgcta ttgtaggttc tattccggca
     3001 gtttttatgc agaaggttat tgctgtggta gaacagaact ggcagtctgg tgactggaat
     3061 ggctgcaagg atgagatcct gcgttataca ggtatccttc ttacacttta tggtctggcg
     3121 ctggcggctt ctatcttcta taaccggaaa atggctgaga tcactcaggg aactttaaag
     3181 aagctgcgtg aaaaaatgtt caacagtatg caggattttc ctgtaaaata ttttgacacc
     3241 cataatcatg gggatattat gagttactac accaatgata ttgacacgct gcgtcagctg
     3301 atctcccaga gtattccgca gtttttaaat tctgctgtta tggtaactac agtattctgc
     3361 gtcatgcttt actatagtat ctggatggca ctggtggtag ttgcaggtgt tgtgctcatg
     3421 ctgcatatta taaagaaagt aggcggaagt tctgcaaaat tctttatcca gcagcagaaa
     3481 gctattggta agacagaagg ttttgtggaa gaaatgatga atggccagaa ggtcattaag
     3541 gtgttctgcc atgaagaaga aagcgaaaag gcttttgatg ctattaatga tgagctgttc
     3601 catgtgtctg aaacagctaa taaatacgca aataccttgg gaccgatctt aaacaatatc
     3661 ggtaacctgc tgtatgtggc agtagctctt gccggaagct tattcctgat gtttaaagtg
     3721 cctaatttaa gcatttcccg attaccattt tctattagta tcgtagtgcc gttcttaaac
     3781 atgaccaaac agtttgcagg aaatatcaat caggtttccc agcagatcaa tgccgttgtc
     3841 atgggtatgg caggtgcaga gcgtattttt ggcctgttag atgaaaagcc ggaggaagat
     3901 gaaggatatg taacactggt aaatgcagtg gaaaatcctg acggaactct ttcagagcac
     3961 aaagaacgta ctggtgtctg ggcatggaaa catccccaca gtgacggaac ggttacttat
     4021 accaggctga caggtgatgt acgttttgaa aatgtagatt ttggttatga tcctggtaag
     4081 acagttcttc ataatattac actgtatgca gaacctggcc agaaggtagc ttttgtaggt
     4141 gcgactggtg ccggaaagac tactatcact aatctgatca atcgttttta tgatatttct
     4201 gatggaacca tccgttatga tggcatcaat atcaggaaga tcaaaaagtc agatctgcgc
     4261 cattctttag gtattgtttt acaggatacc aacctgttta caggtactgt aatggaaaat
     4321 atccgataca gcaacccaga tgcaacagac gaagagtgta tcggagcagc aaaactggcc
     4381 ggcgcccatg attttatcac ccgtctgccg gaaggctatg agacagtgat taccggaaat
     4441 ggtgagaatt tatctcaggg acagagacag ctgatctcca ttgccagagc agccgctgca
     4501 gacccgccag ttatgatctt agatgaggca acatcctcta ttgatacacg tacagaggct
     4561 atcgtacagc ggggaatgga cgccctgatg caggggcgta cagtatttgt cattgcacac
     4621 agactatcca cagtgcgtaa ctccgatgtg atcatggtac tggaacaggg ccacatcatc
     4681 gagcgcggca gccatgataa gctgatcgag gagaaaggaa agtattatca gttgtatact
     4741 ggggcgtttg agttggaatg ataacgcggg tatgtaaata ggatttatag atttgtgaga
     4801 aaaaatgttc gctcggagtc tttctgtgcc tgattttgtg agaagatttt ttgtcagttg
     4861 tgcccatatt tctgaggcat acgcggcgcg tatgttgatg aaatttgggt gcgactggcg
     4921 gaaaatcggc cacaaaggca ggtgcatgaa aggcttcgag aggacatata gaaaaggggg
     4981 aaggcagatg gttgaaagaa aagcgttctt aaaagcctac aacattgacg aagacgattt
     5041 taatgcagct gacatttcct gggaagagct ggcagcaatc tatgatgatt atctgtcaaa
     5101 agaagaaaaa cttcgtggga tcggcaaaga ctttgtcaac gattatctct atgatattga
     5161 gcgggcggga attcattcct accgctaccg taccaaaaat ccgggacatc ttttagagaa
     5221 gatcatacga aaaagaaaag aagatccgga gaaattcaaa gatctgaacc ggagcaatta
     5281 ttacaaatac atgaccgacc tgatcggtat ccgtgtattc ttcctgtacc gggaagactg
     5341 gatctacttc cacaaatata ttaccactgt atttgaaaat gatccaagta tctacgtaaa
     5401 agaccgaggc cgggattttg acgaagatcc gtcccactgc tatattgcag agcgtccaaa
     5461 ggtataccgt agaaatggtg atacacgcat ttatgatgaa aaactgatcg atatcaagtc
     5521 agacggcatt taccgttctc tccactacat tatcaagtat aaaggatatt atgtggagat
     5581 ccagggaaga acgctgtttg aggaaggctg gagcgagatc gatcatgaca tcgtctatcc
     5641 gtattataaa gacgatgaaa tgttaaaaga tttttctacc ttattaaacc gcctttccgg
     5701 tatggcagat gagatgagtt cctatttccg caggatgcgc agtgtgagag aagagcaggg
     5761 gttactgtca caccattcgg gggaaggtaa aaaagaatga tagttcaacc atgacagtaa
     5821 caaaaatacg ctttcttttc caggttccag aaggaaccat agaagagaaa gcgtattttt
     5881 tatatacagc agtgagcaag aatgttatac tcacatgctt tctgttttaa ccagccagat
     5941 gcagtacatt gatcagtaag atccagctta atccatagta agaaacaaca gcaaccaagt
     6001 cgtttacagt tgtgatcagt ggaccagaag caacggccgg gtcaacattg atctttttaa
     6061 agaacagtgg gatacaggta cctacggcac cggagatcac cattgctaaa agtaacgcag
     6121 ctccgataca gccggataca gcataggcga atgcaaaagg ctttcctttt gcgaccacaa
     6181 tgtataagcc aatggctgtg aaagaaagta cgccaaggat tagaccattt gagaaggcaa
     6241 tgcgcatttc cttccatacc agttccagct tctgctttcc ggtaagggac tcgtccataa
     6301 gcacacggat ggtaacagcc agagactggg taccaacgtt tccggccatg tccagaatca
     6361 gggactggaa gcacatgatc agtgtcagct ggcttactac ctgttcaaag aggcctacaa
     6421 cagaggaaac taccataccc agagccaaca gaactaacag ccatggaaga cgtttcttta
     6481 aactttcttt taaaggttcc tttaaatctt cctcagcagt cagacctgcg aacttagcgt
     6541 aatcttcacc catctcatca tcgaccaggt ctacgatact ctgggaagtg ataacgccta
     6601 acagtttgtt gttattatta aggactggga tagagtcctc ggaatagtct ttcagttttt
     6661 caatacagtc atcgatcagt tcatgaccat atacataagg ataagaggta acgatcagat
     6721 cctccaaagc ggagtcctgt cttgcaatga tcagatcctt cagatccatt gcaccataaa
     6781 aggtctgatc ctctgttacc ataaaaatgg tagaaatatt gtcattctta gctgcctgtt
     6841 ctaccagact gctcatagcc tgctttacgg tcagattttc gcggatcaca atacagtttg
     6901 tggtcatcca gctaccgatc tcgtcttcat cgaaggctgc gatcacggca atatcttttt
     6961 ttgcgtcatc atccagaagt tctgtaagaa gagagcgttt ttctttggaa gtcattttca
     7021 aaacatccac aacagcgtct gtttccatag cagaaaggat agcagcagct ttctttacgt
     7081 ccatttcatc cagatattcc gctgcctgtt tctggtcgat atgctcgaaa atatcagaaa
     7141 gcatatctgt atcaaggata cggcagatct tgcgtcgttc agctaaggtc agttccggga
     7201 aaatgtcagc aaggtcgttt tcatggtaat cttccagttt atttctcaga atacccggtg
     7261 aggcattgct tcggattaca gacaggatct cctggctgtg atcctgaact tgaactttgt
     7321 tttccatttc agtctccttt tcatctttat tttattcagt tttcaacacg ccatcctgta
     7381 taaaacatga ttttatgggc acaacaaaac ctgcacctgt aaaatgtgca gaaataataa
     7441 acgcagcatg tatctgtgcg tctgaggata cgcgtgacaa ctaaattgaa agacggtatc
     7501 cgtcctccat ttacagtttt gcgatggata ttacaggagt tgtaagaacg ggataagaaa
     7561 tgaaaaagaa gtcctgtatt tcaagttata tgaaattata cgaaacagaa cagaccgctg
     7621 tttcctttat atcccgtgcg tctacttcgc ccatgactat cgcaaccgtc cattctgttc
     7681 acctgccttt tttgttgaat tttatcattg attttctaac tatcgtgtgc agtatcataa
     7741 ctg
//
LOCUS       DBMDUA010000136         7741 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4165_length_7741_cov_3.006283, whole genome shotgun sequence.
ACCESSION   DBMDUA010000136 DBMDUA010000000
VERSION     DBMDUA010000136.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7741)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4165_length_7741_cov_3.006283"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2358)
                     /locus_tag="ACZPCL_09965"
     CDS             complement(<1..2358)
                     /locus_tag="ACZPCL_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024867.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1162828.1"
                     /translation="MKPKKLVISAFGPYGEKTVVDFEKLGEKGLYLITGDTGAGKTTL
                     FDAITFALYGEASGNVRETGMFRSKYAPDDVPTYVELTFIYQGKDYKVTRNPEYLRPK
                     GRGTGFTLQKAEAELVFPDSRQPVTKTREVTKAITELMGLDYRQFTQIAMIAQGDFQK
                     LLLAGTAERGEIFRQIFHTGIYQDIENRLREAVKGKWKEYDEMRRSISQYLDGVSISD
                     DPEAESEWKELKKAKFEGKTIRGIELLAEVIGRQEEKLAGLKDQIHGIEEEIQKENKV
                     LGEAKQRQKMKTDLEAKQKLFEELKPEVEKAVSQKTAAEEEAKGCEVLAEEIRELREK
                     KAGLEAFLGKVQEAKGKERAIGEEKFTEEKLKQEKVSLEQALLENKEAQEGLKGVEAE
                     KERLQNRLKEAMGKGEKLTGDEKALDQAVKEKKAAEENVLKLEMTGNNWKLQKEEMIK
                     QENALAQAAGNEPLLQMKSQQLQEFLLQAGQLELVQKKLAETQKEYENAVVVRNRERE
                     IYGHLEQTFLDAQAGLLAKHLKEGERCPVCGSDHHPKPAVLTDKVPDKEFLDEKKEVL
                     DQKEENVQKLSALAGQFKEQSEELWEQYRNKAEQIFGDAGAESLPEVKRRLSEAINNC
                     DQKLKDAQKAAESLKKCQETKEALETNEKILQDKLLQARNIHSAAEGQMDLLIRQSIK
                     NLQDEPKIQEMQQVPGIQSTNDIENVVSRIKMFFAELKAQNNTVLEQIKADLEENGKK
                     AEYLNRLQAQAETMEDQQKKLSENISEKALLIQKLATQLEELKKQL"
     gene            complement(2355..3503)
                     /locus_tag="ACZPCL_09970"
     CDS             complement(2355..3503)
                     /locus_tag="ACZPCL_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004529 - DNA exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HIR1162829.1"
                     /translation="MKLFHLSDLHIGKRVNEFSMLEDQKYILMQILKAAKEHQPDGII
                     LAGDIYDKPVPAAEAVQVFDDFLTSLNEMGLIVFMISGNHDSPERVSYGGRLMKKSGI
                     YVAPVYEGKTEKVEFTDAYGKVCIHLLPFVKPAVVRHAFEEGEFEKEAEAVCDYQSAV
                     KMTVSHMAVDTDVRNVLIAHQFVTGAMRCESEEVSVGGLDNVDASVFDGFDYVALGHI
                     HSPQAVGRDQVRYCGTPLKYSFSEAGQEKSITVVELKEKGNVEISAVPLHPLRDMRKI
                     RGSYMEVTAKSFYENGNCEDYLHVTLTDEEDILDGLQKLRVIYPNIMQLEYDNRRTRE
                     NPELTAAQAVEQKSEMELFMEFYELQNNQPMSQIQEEFVRNIIREVKE"
     gene            complement(3832..5211)
                     /gene="argH"
                     /locus_tag="ACZPCL_09975"
     CDS             complement(3832..5211)
                     /gene="argH"
                     /locus_tag="ACZPCL_09975"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR1162830.1"
                     /translation="MKLWGGRFTKETDQLVQNFNESLSFDQKFYRQDIRGSIAHVKML
                     AKQGILTEADRDAIVAGLEGIREDLDSGKLVIPADSEYEDIHSFVEGTLTERIGEAGK
                     RLHTGRSRNDQVALDMKLYTRDEIDEMDVLVKDLLKELLVIMEANTETFMPGFTHLQK
                     AQPVTLAHHMGAYFEMFSRDRSRLQDIRKRMNYCPLGSGALAGTTYPLDREYTAKLLD
                     FDGPTLNSMDSVSDRDYLIELLSALSTIAMHLSRFSEEIIIWNTNEYRFVEIDDSYST
                     GSSIMPQKKNPDIAELIRGKSGRVYGALVSMLTTMKGLPLAYDKDMQEDKELTFDAID
                     TVKGCLSLFTGMISSMKFRKDVMEASAKNGFTNATDAADYLVNHGVPFRDAHGIVGQL
                     VLLCIDKGIALDDLPLEEYKKISPVFEEDVYEAISMKTCVEKRMTIGAPGPEMMKKVI
                     EIYKKQLEQ"
     gene            complement(5484..>7741)
                     /locus_tag="ACZPCL_09980"
     CDS             complement(5484..>7741)
                     /locus_tag="ACZPCL_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIR1162831.1"
                     /translation="SALYAYYLASPVNWLIILCPKKFIIEFMTILIMVKTGLCGVTFT
                     WYLDHHFEKKHFVAGVFGIFYALSGYMAAYSWNIMWLDCIFLLPLILYGLEQLVQEKK
                     GLFYCVTLGLSILSNYYISIMICIFMVMYVIVQVILYPPKKIKDLVATGVRFGFYSLL
                     AGGLAAVVLLPEIYALQATASGDFDFPKTISCYFSIFDMIARHIGNVGTEIGLDHWPN
                     IYCGVAVLMFLLLYLGAKKITVREKAVYCGLLLMFYASFSINVLNFIWHGFHYPNSLP
                     CRQSFIYIALVLTMCYQVCLELKETSWKQVVWAFWGAIIFVLMAEKLVDNSAQFHFSV
                     FYAAIIFLALYMGLIYLYKRKNWNEDVIMMLTLLLVAVETALNLGVSSLPTTSRTAYV
                     KDNQDTRRLAESIKCDTFYRVEKGDSKTKNDGAWMNFPSASLFSSVANASLTDFVRKL
                     GCESSTNAYSIKGSTPLVDSLFSIRYGIYPDQQPVTGLKDQISRKGSMWLYENQNTLP
                     VAFMLPADVENNWLLDSGNPAVVQNDLCNVLGTKDVLVSNESVTEGKKLTFTVETSGD
                     YYVYVTNKKVEKVTATMGEKSRSFDNVDRGYFLELGYLVKGTDVELRSEDEGNPSLQG
                     EVWRFDPEAFKEFYDKMNENPINLTKWTDTELSGTIHSDGYKTMFTSIPYDKGWKLFV
                     DGVETETRSVFDTFIAADLAEGDHEISFTYEPEGLKTGAIITGVSLVVFAGAAIITTV
                     NNKNKKKKTKI"
BASE COUNT         1886 a         2035 c         1430 g         2390 t
ORIGIN      
        1 cagctgcttc tttaattctt ccagctgagt tgccagcttc tggatcagca gggccttttc
       61 ggaaatattt tcagaaagtt ttttctgttg gtcttccatc gtttcagcct gggcctgtaa
      121 acggtttaag tattccgcct tttttccatt ttcttccaga tcagctttta tctgttctaa
      181 aacagtgttg ttttgggctt ttagctctgc gaaaaacatt tttatcctgg atacaacgtt
      241 ttctatatca tttgtgctct gtatccccgg tacctgctgc atttcctgta tcttcggctc
      301 gtcctgcaaa ttttttatgc tctgtctgat caacagatcc atctgccctt ctgccgctga
      361 atggatattt cgggcctgca gcagtttatc ctgaagaatc ttctcattgg tctccaatgc
      421 ctctttcgtt tcctggcatt ttttaagact ttccgctgcc ttttgggcat cctttaattt
      481 ctgatcacag ttattaattg cttctgacag acgccgtttc acctcaggta gactctctgc
      541 ccctgcatcc ccaaagatct gctccgcctt attccggtac tgttcccaca gttcttccga
      601 ctgttcctta aactgccccg ccaatgcact tagcttctgg acattttctt ctttctgatc
      661 caatacctct ttcttctcat ctaaaaattc cttatccggc accttatctg taagtactgc
      721 cggttttgga tgatgatcgg aaccgcagac cgggcatctc tccccctctt ttaaatgctt
      781 tgccagaagc cctgcctggg catctaaaaa tgtctgttcc agatgaccat aaatttctct
      841 ttccctgttt cttacaacaa cggcattttc atattctttc tgggtttctg ccagcttttt
      901 ctggaccagt tccaattgac ctgcctgaag caaaaactcc tgtaactgtt gtgacttcat
      961 ctgtaaaaga ggctcatttc ctgctgcctg cgccaaagca ttttcctgct ttatcatttc
     1021 ttccttctgc agcttccagt tatttccagt catttccagt ttcagcacat tttcctctgc
     1081 cgcctttttc tcctttacag cctgatccag agctttttca tccccagtca gtttctcccc
     1141 ttttcccatg gcttccttaa gcctgttttg cagccgctct ttctctgctt ctacaccttt
     1201 cagaccttcc tgggcttctt tattttcaag cagtgcctgt tccagagaaa ctttctcctg
     1261 ttttaacttc tcttccgtaa acttctcttc tcctattgcc ctttcttttc ctttggcttc
     1321 ctgtactttt cccagaaatg cctccaagcc ggcttttttc tctctcagct ctcttatctc
     1381 ttctgccagt acttcacagc cctttgcctc ttcttctgcc gccgtctttt gagaaacagc
     1441 cttttctacc tcaggtttta gttcttcaaa cagcttctgc tttgcctcca gatctgtttt
     1501 catcttctgt ctctgctttg cttccccaag aaccttattt tctttctgta tctcttcctc
     1561 gatcccatgg atctgatctt tcagacctgc aagtttttcc tcctgccgtc ctataacctc
     1621 agccagaagt tcaattcccc gaatggtctt tccctcaaat ttcgcctttt tcagctcttt
     1681 ccattcactt tctgcttccg ggtcatcact gatgctgacg ccatccagat actggctgat
     1741 gctgcggcgc atctcatcat attctttcca ttttcccttc actgcctccc gcagcctgtt
     1801 ctcaatatcc tgatagatcc ctgtatggaa gatctggcgg aagatctccc ctctctctgc
     1861 agttccggct aaaagcagtt tctggaaatc tccctgggcg atcatggcga tctgggtaaa
     1921 ctgtcggtag tccagtccca ttaactctgt aatggctttt gtcacttctc tggtcttggt
     1981 cacaggctgc cggctgtctg gaaaaacaag ttctgcctct gctttttgca gggtaaatcc
     2041 tgttcctctg ccctttggac ggagatattc cggatttctg gtgactttat aatcttttcc
     2101 ctgataaata aaggtcagct ccacataagt aggcacatca tccggtgcat acttgctgcg
     2161 aaacatcccc gtttccctga catttccgct ggcttcccca taaagagcaa aagtaatggc
     2221 atcaaaaaga gtggtctttc ccgcacctgt atcaccggtg atcagataaa gccctttttc
     2281 tcccagcttt tcaaaatcga ccactgtctt ttccccataa ggcccaaagg cgcttataac
     2341 cagttttttc ggcttcattc tttcacctcc cggatgatat ttcgcacaaa ctcttcctgg
     2401 atctggctca tgggctggtt gttctgcagc tcataaaact ccatgaacag ctccatttct
     2461 gacttctgtt ctactgcctg ggctgccgta agctccggat tttccctggt cctgcggttg
     2521 tcatattcca gctgcataat attggggtaa atgacccgca gcttctgcag accgtctaaa
     2581 atatcctctt catcggtcag tgtcacatga aggtaatcct cgcagtttcc attttcataa
     2641 aatgactttg cagtcacttc catatagctg ccccggatct ttcgcatatc ccgcagggga
     2701 tgcaatggaa cagctgagat ctccacattt cctttttctt ttaattccac cacagtaatg
     2761 gatttttcct gacccgcctc tgaaaaagaa tatttaagag gtgtgccaca gtatcgcacc
     2821 tggtctcttc ccactgcctg gggactgtgg atatgcccca gggccacata atcaaaccca
     2881 tcaaatacag aagcgtccac attgtcaagg cctcctacgg acacttcctc tgactcacag
     2941 cgcatggcgc cggtgacaaa ctggtgggcg atcaacacat ttcgcacatc tgtatccaca
     3001 gccatatggg aaacagtcat tttcactgca ctctggtaat cacacactgc ctctgcttct
     3061 ttttcaaatt ctccctcttc aaaagcatga cggactacag ccggttttac aaaaggaagc
     3121 agatggatgc acacttttcc atatgcatcg gtaaattcca ccttttctgt ctttccctca
     3181 tacacaggcg ccacgtaaat accgcttttc ttcatcagcc gtccgccgta ggaaacacgc
     3241 tccggtgaat catggtttcc gctgatcata aacacgatta gtcccatttc atttaaactt
     3301 gtaagaaaat catcaaatac ctgcactgcc tctgccgccg gaaccggctt atcataaata
     3361 tcacctgcta aaatgatccc atctggctga tgctcctttg cagcttttaa gatctgcatc
     3421 aggatatatt tctggtcctc cagcatggaa aactcattga cccgctttcc aatatgcagg
     3481 tccgataaat gaaacaactt catagtaacc tcccgaccat aatatgttct ctcggaatct
     3541 ttctgtgcct gattttgtga gaagattttt tgtcagttgt gcccaaattt ctgaggcgta
     3601 cgcggtgcgt acgttgatga aattcgggtg cggctggcgg aaaatcagcc acaaaggcag
     3661 gtacaagaaa gactccgaga ggacattaat aaatccgtga acgcgccgcc ggcgcattct
     3721 caatatgtat agcaaaaagg tgccgcaaaa catctcctct tttgcggcac ctctcattcc
     3781 acctagtatc tttccactca gtatctattt ccagtttatt atgtttatta cttactgctc
     3841 taactgcttc ttatagatct caattacttt cttcatcatt tccggacctg gtgcgccgat
     3901 ggtcatacgc ttttcaacgc aggtcttcat gctgatggct tcgtatacat cttcctcaaa
     3961 tacaggactg atcttcttgt attcctctaa tggaagatca tctaaagcaa tgcccttgtc
     4021 gatgcaaagc agtaccagct gtcctacgat accatgggca tcacggaatg gtacgccatg
     4081 gtttaccaga taatcagctg catccgttgc attggtaaag ccattctttg cgctggcttc
     4141 cattacatcc ttgcggaact tcatggaaga gatcataccg gtaaataagg acaggcagcc
     4201 cttcactgta tcaatagcgt caaaggtcag ttccttatct tcctgcatat ccttatcata
     4261 tgccagagga agtcccttca tggtagtaag catggaaacc agggcgccgt aaacgcggcc
     4321 gctctttcca cggatcagct ctgcaatatc cgggttcttc ttctgaggca taatactgct
     4381 tccggtgctg taggagtcat cgatctctac aaaacgatat tcattggtgt tccagataat
     4441 gatctcctcg ctgaaacggc ttaaatgcat ggcaatagta gataatgcac ttaacagctc
     4501 gatcagatag tcgcggtcag aaacggagtc catggagttt aaggtaggac cgtcaaaatc
     4561 cagaagtttt gcagtgtatt ctctgtccag cggataagta gttccagcca gagcgccgga
     4621 gcctaacggg cagtaattca ttctcttgcg gatgtcctgt aaacgagaac ggtctctgga
     4681 gaacatttca aaatatgcgc ccatgtgatg agccagtgtt acaggctgtg ccttctgtaa
     4741 atgggtaaaa ccaggcataa atgtctcggt gtttgcttcc atgatcacca gaagttcttt
     4801 taaaagatcc tttacaagaa cgtccatctc atcaatctcg tctcttgtat acagcttcat
     4861 atccagagct acctgatcgt tacggctgcg tcctgtatgc aggcgctttc ctgcttcgcc
     4921 gatacgctct gttaaggttc cttctacgaa agagtggata tcttcgtatt cggagtcagc
     4981 cgggatcacc agcttaccgc tgtcaagatc ttctctaatg ccttccagac ctgccacaat
     5041 ggcatctctg tctgcttctg taagaattcc ctgtttcgct aacatcttta catgtgcgat
     5101 gctgcctctg atgtcctgac gataaaattt ctgatcaaat gaaagtgatt cattaaaatt
     5161 ctgtaccagc tggtctgttt cttttgtgaa acgtccgccc caaagcttca taattgatgt
     5221 ctccttttgt cctttaattt ataatctgca tgtcctttcg gaacatttct gcatgtatga
     5281 taccagattg ctacgtgctt tgcactctcg caatctttaa aacattcgta atccgctcat
     5341 ttttctttga aaaatggcta cttactcatg ttttacgtgc cccaagttcc aaaatcctcc
     5401 tgcaagcaag cttgcgttgg attttagacc ttttgacact tggtatcatt ataccttata
     5461 aattcacatt ttgcggctat tttttatatt tttgtctttt ttttcttatt tttattattt
     5521 acagtagtaa tgatcgctgc tcctgcaaac actacaaggc tcacacctgt aataatagca
     5581 cctgttttta aaccttccgg ctcataggta aaggaaattt catggtctcc ttctgccagg
     5641 tctgctgcaa taaatgtatc aaaaacagat cttgtttccg tttccacacc atccacaaaa
     5701 agcttccagc ccttgtcata tggaatactt gtaaacatgg tcttatatcc atcagaatga
     5761 atggttccgg aaagttctgt atccgtccat ttagtcaggt tgatgggatt ttcattcatt
     5821 ttatcataaa attctttaaa ggcttccgga tcaaaccgcc atacttctcc ctgaagggaa
     5881 ggatttccct catcctctga gcgaagttct acatccgttc cctttacaag atatcccagt
     5941 tccaggaaat aacctctgtc tacattgtca aaggacctgc tcttttctcc catggttgct
     6001 gtgacctttt ccactttctt gttggtcaca tacacataat aatcaccgga agtttccact
     6061 gtaaaggtaa gtttcttgcc ttctgtaaca ctttcattgg acaccagcac atctttggtc
     6121 cccagcacat tgcaaaggtc attctgtact accgcaggat tgcctgagtc taacagccag
     6181 ttattctcca catctgcggg aagcataaat gctaccggca gcgtattctg attttcataa
     6241 agccacatag agccttttct gctgatctga tcctttaaac cagttaccgg ctgctgatcc
     6301 ggataaatgc catatcggat agaaaacagg ctgtctacca ggggagtact tcctttaata
     6361 ctgtaagcat tggtggaact ttcgcagccc agctttctca caaaatcagt taaagaagca
     6421 tttgctaccg atgaaaacag ggacgcagat ggaaaattca tccacgcacc atcatttttc
     6481 gtcttgctgt ctcccttttc cactctgtag aaagtatcac atttaatgct ttccgctaac
     6541 cttctggtat cctgattgtc ctttacatac gcagtcctgc tggtagttgg aaggctgctg
     6601 acaccaaggt ttaacgcagt ttccactgct actaaaagca gggtcagcat catgatcaca
     6661 tcttcattcc agtttttccg cttatacaga taaataagcc ccatatacag tgccaggaaa
     6721 atgatggctg cataaaacac agaaaaatga aattgtgcag agttatccac aagtttctct
     6781 gccatgagca caaagataat ggcaccccag aacgcccaca ccacctgttt ccaggaagtt
     6841 tctttcaatt ccaggcagac ctgataacac atagtcagca ccaaggcgat atagataaag
     6901 gactgtctgc atggaaggct gtttggataa tggaacccat gccagatgaa atttaacaca
     6961 ttgatggaaa agctggcata aaacattaac agcagaccgc agtaaactgc cttttcccgc
     7021 actgtgatct tctttgcccc cagataaaga agaaggaaca taagcactgc cacaccgcag
     7081 taaatattgg gccagtggtc caggccgatc tctgttccca catttccaat gtgacgggcg
     7141 atcatatcaa aaatggagaa ataacagctg atggtctttg gaaaatcaaa gtcgccggaa
     7201 gcagtcgcct gcaaagcata gatctcaggc aaaagcacaa cggctgccag tcctcctgca
     7261 agaagagaat aaaagccaaa gcgcactcct gttgccacca ggtcttttat cttctttggc
     7321 gggtataaga tcacctgcac gatcacatac atgaccatga aaatacagat catgatagag
     7381 atatagtagt tggacagaat agaaagaccc agagtcacac agtaaaacag gccctttttc
     7441 tcctgcacca gctgctctaa cccataaagg atcagcggca gcaggaaaat gcagtccagc
     7501 cacatgatat tccagctgta agctgccata taaccgctta aagcatagaa aatgccgaaa
     7561 acaccggcta caaaatgctt cttctcaaaa tgatgatcca gataccaggt aaacgtcact
     7621 ccgcagagac ctgtttttac cataataagg atggtcataa attcaatgat gaatttcttc
     7681 ggacataaga tgatcagcca gttcaccgga cttgccagat aataagcata aagagccgag
     7741 a
//
LOCUS       DBMDUA010000137         7598 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4221_length_7598_cov_3.635854, whole genome shotgun sequence.
ACCESSION   DBMDUA010000137 DBMDUA010000000
VERSION     DBMDUA010000137.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7598)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4221_length_7598_cov_3.635854"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            271..2256
                     /gene="tkt"
                     /locus_tag="ACZPCL_09985"
     CDS             271..2256
                     /gene="tkt"
                     /locus_tag="ACZPCL_09985"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR1162832.1"
                     /translation="MGMIETKTANAIRVLSADAIQKAKSGHPGLPLGCASAAYELWAN
                     HMRHNPADPKWVDRDRFILSGGHGSMLLYSLFHLFGYGDLSMEDLKNFRQIDSLTPGH
                     PEYGHTVGVEATTGPLGQGMGMAVGMAMAEAHLASVFNKDDIKIVDHYTYVLGGDGCM
                     MEGLSSEAFSLAGTLGLGKLIVLYDSNNISIEGNTDIAFTEDTAKRFESYGFGVFQVE
                     DGNDFAAIGAAIEAAKADTERPSLIVLHTQIGYGCPAKQGKASAHGEPLGEENIIAMK
                     ENLGWESMEPFYVPQDVYDHMAKVRESLKAPEEEWNARFAAYCEKYPEMKELWDQYHD
                     KDLPKKLWDNEEFWSYEDKPQATRNLSGELLNKINKVVPNLFGGSADLAPSNKTNLKG
                     EGDFSKADYSGKNLHFGVREQAMTAIGNGLVLHGGVIPYVATFFVFSDYMKPIARLSS
                     LMKVPLIYVLTHDSIGVGEDGPTHEPIEQLAMLRAQPNFHVFRPADAKETAAAWYSAI
                     TSEKTPTALVLTRQNLPQLAGTGRDAVKGAYIVADSAKETPDAIIIATGSELSLAVEA
                     KELLLKDGMDVRVVSMPCMDLFEEQSPEYKESVLPKAVRKRVAVEALSDFGWGRYVGL
                     DGATVCMEGFGASGPAAQLFEKFGFTAERVAETVKSL"
     gene            complement(2430..2696)
                     /locus_tag="ACZPCL_09990"
     CDS             complement(2430..2696)
                     /locus_tag="ACZPCL_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162833.1"
                     /translation="MFRMWGKIMKDNHLVKDMVACCGDYSISRTQMVFNCLDEICYKF
                     DLEKPMWLDATVQDFKYHDKARFTQDNFIETVDFDYLEIQVIEE"
     gene            2819..3553
                     /locus_tag="ACZPCL_09995"
     CDS             2819..3553
                     /locus_tag="ACZPCL_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013776.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1162834.1"
                     /translation="MPYWAEKDDALLSVLQKYIDRDWNDKDLKVILCGSALSFMEKKV
                     LSEKSPLFGRRDSQIKLEAFNYLDAAKFVPNYSNEDKAICYGITGGVAKYLSMIDPKK
                     SMDENIVRLFFRTDGYLYDETRNLLTQEFSDISLVNNIVEQIAFGENTLNTIAGKIGE
                     KEPTVLYSLDKLINVGLVEKRKCITDEKNKKKTQYVLRDYMFKFWYEFIPKATSVIEM
                     GQGEIYYQDTQSGLKHYQKRMYCCLH"
     gene            3825..4931
                     /locus_tag="ACZPCL_10000"
     CDS             3825..4931
                     /locus_tag="ACZPCL_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162835.1"
                     /translation="MEQAITDYVKFLADARDAVYRLNNDQATAKQLKEQGERQEKELA
                     AAKKAVTDSVNQTIKKRRSEIDTSYDKEIAKGQERLKKARTQREKAKNKGMKERIAEE
                     TSELREHNRDLQIQMKTMFQKDKVPVFCRTTFYYSLYYARGLKERLIGFITFLICFLA
                     LPCGVYFLLPDRKIWYLVLVYFADIIIFGGLYVTIGNRSRSRYHDALKQGREIRNLLS
                     SNEKKIKVIVHSIEKDGNEDIYDLEKYDDEIACAQQELSDIAAKKKDAISSFENVTRN
                     IIADEIEGNHRAEIEEKEQKLQEVNDSLAQLESSIRQQNIHITDTFGPYLGKEYLNTD
                     KLLGLSKLIQSGTASNITEAIEMDKNGVGKTETN"
     gene            4985..5557
                     /locus_tag="ACZPCL_10005"
     CDS             4985..5557
                     /locus_tag="ACZPCL_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HIR1162836.1"
                     /translation="MYRVVIADDEVLIRMYIREILENNGYEVVGEAEDGLDAIAVCRQ
                     KKPDFVIMDINMPVMTGLEATKVINEDKLAGFVIILTAYRDKEIADQAVNTDVMGYIV
                     KPVDEDTLIPAVKIAIHNYKQREAMAKEFHKTQEALDDRKYLDRAKGLVMERKNMSEK
                     EAFTYIRSLSMDKGISMTELSKMLLKAYEG"
     gene            5580..7043
                     /locus_tag="ACZPCL_10010"
     CDS             5580..7043
                     /locus_tag="ACZPCL_10010"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF023702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1162837.1"
                     /translation="MEQKKNKLLSDMIQNDTDKKMVQLVEAATDLSCGDICTLLEVAH
                     SLPFVGNLEGGDTYINVLTREKESMVIAQYRHPNYDLYKRSIIGEIERREDEPAVYRA
                     LEEGISGRGLIGIIDEGRTVVRHTVSPILNSEKKVIGALTYEYPNAGADTESIRIINN
                     QEGKQDPFNRQLGKASDYLQDAILLYDANGICTFVNPKAEVLYQDKGFELPLIGRRCS
                     ELHITDCNWSDLTEHRGVIRREVRTPNFIMDAVISGIWENGTCQGAAIIFRDKTVVHQ
                     MEDEIAYRVALIHEVHHRVKNNLQTIISLIGLEAVHTKDEKVKAFAKTITSHVRSMNI
                     TYDLLSHTGSENVGLKVLISRITDVLLENNCLKETCSISTRVDGDDVILTETTASTVA
                     LIVNELVQNSLKYAFKDRKQGQIRLKIEKGASYSWITVQDDGCGFDNKKISRANSGLG
                     LRLISSLVQSSLKGELFIETGENGTSIRFSFSMPQAG"
BASE COUNT         2403 a         1420 c         1865 g         1910 t
ORIGIN      
        1 atattcataa gactattatg agatagattt tagaaaaaag aaacccctaa cccctcaaga
       61 ttgaggggca ggggagttga ataggcgaag cctatttttt agctgaaata ttgaattttc
      121 aaggtgcgaa gccctgttta ggtgtgggaa gttttagtaa ataataaaaa ttagaatgtt
      181 aacatgggaa aacacagact agacgtaaac caattccaat ggtagaattg acatagatta
      241 cgttgtgaaa acgaaaggag aactattaaa atgggcatga tagaaacaaa gacagctaat
      301 gccattcgtg tattatctgc tgatgctatc cagaaggcaa aatccggaca tcctggactt
      361 cctttaggat gtgcttccgc agcttatgaa ttatgggcta atcatatgag acataacccg
      421 gcagatccaa agtgggtgga cagagaccgt ttcatccttt ctggcggaca tggttctatg
      481 ctgctgtatt cattattcca tttatttggc tatggtgatc tgtctatgga agatttaaag
      541 aacttccgtc agatcgactc ccttacacca ggacatcctg agtacggcca cacagtaggt
      601 gtagaagcta ctacaggacc tttaggacag ggaatgggca tggctgtagg tatggctatg
      661 gcagaggctc atttagcttc cgtattcaac aaagatgaca ttaagatcgt tgaccattac
      721 acctatgtac tgggcggaga cggctgtatg atggaaggtc tttcctctga agccttctct
      781 ttagcaggta ccttaggact tggaaaactg atcgttctct acgactctaa caatatttcc
      841 atcgaaggaa atacagatat cgcatttaca gaagatactg caaagcgttt tgagtcctat
      901 ggatttggcg tattccaggt agaagatgga aatgacttcg ctgctatcgg cgcagcgatc
      961 gaagcagcta aggctgatac agaaagacct tctctgatcg ttcttcatac tcagatcgga
     1021 tacggctgcc cggctaaaca gggtaaagca tctgcacatg gtgagcctct gggcgaagag
     1081 aacatcattg ccatgaagga aaacttaggc tgggagagca tggagccatt ctatgtacct
     1141 caggatgttt atgaccacat ggctaaggta agagaaagct taaaggcacc agaagaagag
     1201 tggaatgcaa gatttgcagc atactgtgag aagtatccgg aaatgaagga actttgggat
     1261 cagtatcatg acaaagacct gcctaagaag ctttgggaca atgaagaatt ctggtcctat
     1321 gaggacaagc cacaggcaac cagaaatctg tccggcgagc tgttaaataa gatcaataag
     1381 gtagttccga acctgttcgg cggttctgcc gacctggctc cttccaacaa gaccaactta
     1441 aagggcgagg gagacttctc caaggctgat tactctggaa agaacctgca ctttggtgta
     1501 agagagcagg caatgactgc tattggaaat ggtctggttc ttcacggcgg cgtgatccct
     1561 tatgtagcaa ccttcttcgt attcagtgat tacatgaagc caattgcaag actgtcatcc
     1621 ctgatgaagg tgccgctgat ctacgttctg acccatgaca gcatcggtgt aggcgaagac
     1681 ggacctaccc atgagccgat cgagcagtta gctatgttac gtgcgcagcc taacttccat
     1741 gtattccgcc ctgcagatgc aaaagagact gcagcagcat ggtacagtgc gattacttct
     1801 gaaaagacac ctaccgctct ggtacttacc agacagaacc tgcctcagtt agcaggaact
     1861 ggccgtgatg cagtaaaggg cgcttacatt gtagctgatt ccgcaaagga aactccagat
     1921 gcgatcatca ttgctaccgg ttctgagtta agcctggcag tagaagcaaa agaactgctg
     1981 ttaaaggatg gaatggatgt ccgcgttgta tccatgccat gtatggacct gttcgaagag
     2041 cagagcccag aatacaaaga gtccgttctt ccaaaggctg taagaaagag agttgcagta
     2101 gaagcacttt ctgacttcgg ctggggccgt tatgtaggcc ttgacggcgc aactgtatgc
     2161 atggaaggct tcggtgcatc cggccctgca gcacagctgt ttgagaagtt tggatttacc
     2221 gcagagcgtg ttgctgagac tgtaaagagt ctgtaagacg taaaaatcga tggatgcgcc
     2281 gaaggcgcat cctgatatcg caataataag ataccccggt gaccgcctga aaaatggcag
     2341 ctgccggggt gtttttgtct caatggtgca tacttgtgca atgaaaaagt ttgacacaag
     2401 cgaccgcgcc aaaggcgcgg ttaaccgttt tattcctcga tcacctgtat ctctaaataa
     2461 tcaaaatcca cagtctcaat aaaattatcc tgggtaaacc gggctttgtc gtgatattta
     2521 aaatcctgga ctgtagcatc cagccacatg ggcttttcca ggtcaaactt atagcatatt
     2581 tcatctaaac aattaaaaac catctgggtt ctggaaatag aataatcgcc gcagcaggcc
     2641 accatatctt tcaccagatg gttgtctttc atgatctttc cccacatccg aaacatgtat
     2701 tttccagcct ttcttttttg ttgaaccgtg tgtacatgtt agttagtata gcatagaata
     2761 ctgcgtataa tctataaaaa acggctgcaa tcagaatatt ctttcatgca gccatgaact
     2821 gccatattgg gccgaaaaag atgacgctct tttatctgtt ttgcagaaat atattgatag
     2881 agactggaat gataaggacc ttaaagtgat cttatgtggc tctgcactta gctttatgga
     2941 gaaaaaggta ctcagtgaaa agagccctct gtttggaaga cgtgattcgc agatcaaatt
     3001 agaggcgttc aattacttag atgctgcgaa atttgttcca aactattcaa atgaggataa
     3061 ggcaatttgt tatggaatta ccggtggtgt ggcaaagtat ctttcaatga tcgatcctaa
     3121 aaaaagtatg gatgaaaata ttgtaaggct attttttaga acagatggat acctgtatga
     3181 tgaaacaaga aatcttctga ctcaggaatt ttcggatata tcccttgtaa ataatattgt
     3241 tgagcagatc gcgtttggcg aaaatacgct taatacgatt gcgggaaaaa ttggagaaaa
     3301 agaaccaaca gtattatact ccctggataa attaataaat gttggtctgg ttgaaaagag
     3361 aaagtgtatt acagatgaga aaaataaaaa gaagacccag tatgttttaa gagattatat
     3421 gttcaaattc tggtatgagt ttatcccaaa ggctaccagc gtgatcgaga tgggacaggg
     3481 agagatttat tatcaggata cacagagtgg tttgaagcat tatcagaaga ggatgtattg
     3541 ttgtttacat tagattcact ttatgaataa aaataatatt aaatcatgtt taaaagcagt
     3601 acctgttatg ggcggacatg ccgcttgctg ggtaaaagca aagccaaatg cattctctgg
     3661 ggagaaggaa aatgcgaaag taaagatcca cctttgcggc ggcgtggaaa caggggccag
     3721 ctgccaaaca gacagctggc ccttgccctg ttacaggttt tgtagtaagc tataactatt
     3781 gacaaaatcc cccgaaattc gggaggagag gaggtacttg ggcggtggag caggctatta
     3841 ctgattatgt gaaatttctg gcagatgcca gggatgcagt gtatcgtctg aataatgatc
     3901 aggcgacagc aaagcagtta aaagaacagg gagagcgcca ggaaaaagag ttggcagctg
     3961 cgaaaaaggc agtgacagat tcggtgaacc agaccataaa aaagcggcgc agcgagatcg
     4021 atacaagcta tgacaaagag atcgcaaaag gtcaggaacg gctgaaaaaa gccaggaccc
     4081 agcgggaaaa agccaaaaac aagggaatga aggaacggat cgcagaggag accagtgaac
     4141 ttcgggaaca caacagggac ttgcagatcc agatgaagac catgttccag aaggataaag
     4201 tgcctgtatt ctgccgcact acattttatt attccctgta ctatgcaaga ggacttaagg
     4261 agcgtctgat tggttttatt acgttcctga tctgcttttt agcacttcca tgcggtgttt
     4321 atttcctgct tccggaccgt aagatctggt atctggtact ggtttatttt gcagatatca
     4381 ttattttcgg cggtctgtat gtgaccattg gaaaccgcag ccgttcccgt tatcatgatg
     4441 ccttaaagca gggccgggag atccgcaacc tgttaagctc caatgaaaag aagatcaaag
     4501 tcattgtcca cagtattgaa aaggacggca atgaagatat ttatgatctg gagaaatatg
     4561 atgatgagat tgcctgcgca cagcaggaac tgtctgatat agcagcaaag aaaaaagatg
     4621 ccatcagcag ctttgaaaat gtgaccagaa atattattgc cgatgagatc gaggggaacc
     4681 acagggcaga gatcgaagaa aaagaacaga aattacagga agtaaacgac agtcttgctc
     4741 agttagaaag cagcatccgc cagcagaaca tccatattac agacaccttt ggtccatatc
     4801 ttggaaaaga gtacctgaat acggataagc ttttaggatt gtctaagttg atccagtccg
     4861 gaacagcttc caatattacg gaggctattg aaatggataa aaatggggta ggaaaaacag
     4921 aaacaaatta agataacaga cagaagagag agacagaata agcagacaat gtaaggggga
     4981 caatatgtac agagtagtga tcgcagatga cgaagtgctg atccgcatgt atattcggga
     5041 aatcttagaa aacaatggtt atgaagtagt aggagaagca gaagacggtc tggatgcgat
     5101 cgcagtgtgc agacagaaaa agccggattt tgttattatg gacattaata tgccggtgat
     5161 gactggtctg gaagcaacga aagtgatcaa cgaggataag ctggctggat ttgttattat
     5221 tttgacggct taccgtgaca aggaaattgc tgatcaggca gtcaatacag atgtaatggg
     5281 atatattgta aagccggtag atgaagatac actgataccg gcagtaaaga tcgcgatcca
     5341 taactataag cagagagaag ctatggcgaa ggagtttcac aagacgcagg aagccctgga
     5401 tgatagaaaa tatttagacc gggcaaaagg tctggtgatg gagcggaaaa acatgtctga
     5461 aaaagaagca tttacttata tccgcagtct gtccatggat aaggggatct ctatgacgga
     5521 actgtcaaaa atgcttttga aagcatatga aggctgacac aaaagcagag agggaattta
     5581 tggaacagaa gaaaaataag cttctcagtg acatgataca aaatgataca gataaaaaaa
     5641 tggtgcaact ggtagaagcg gcaacggatc ttagctgcgg ggatatctgc acccttttag
     5701 aggtggcgca ttccctccct tttgtgggaa atctggaagg cggagataca tacatcaatg
     5761 tgcttacaag agaaaaagag tccatggtca ttgctcagta cagacatcca aactatgatc
     5821 tgtataaacg cagtatcatt ggggagattg agcgcaggga agatgagccg gctgtgtacc
     5881 gggcgctgga agaaggcatt tccggccgtg gactaatcgg catcattgat gaaggaagaa
     5941 cggtagtgcg gcatactgta agccctatac ttaacagcga aaaaaaggta ataggggcgc
     6001 tgacatacga atatcccaat gccggtgcag atacagagtc tatccgtatt atcaataatc
     6061 aggaaggaaa acaggatcct tttaacaggc agcttggaaa agccagcgat tatcttcagg
     6121 atgccattct tctttatgat gcaaatggaa tatgtacatt tgtcaatccc aaggcagaag
     6181 ttctgtatca ggataaggga tttgaactgc ctttgattgg ccgcagatgc agcgaactgc
     6241 acattacgga ttgcaattgg tctgatctta cagaacatcg tggcgtaata cggagagagg
     6301 tacgtacacc gaatttcatc atggatgcgg tcatcagcgg tatctgggaa aatggcacct
     6361 gccagggcgc agcgattatc ttccgggata agacagtggt acatcagatg gaggatgaga
     6421 tcgcttaccg cgtggcgctg atccatgagg tacatcaccg ggtcaagaat aatcttcaga
     6481 cgattatcag tctgatcggg ttggaagcgg tccatacaaa ggatgaaaaa gtaaaagctt
     6541 ttgcaaagac cattaccagt catgtgcgca gcatgaatat cacttatgac ctgctttctc
     6601 atacaggaag tgaaaatgtg ggattaaaag tcctgatctc ccgtattaca gatgttttac
     6661 tggaaaataa ctgtttaaaa gaaacgtgca gtatttctac aagggtagat ggggatgatg
     6721 tgatacttac agaaacaacg gcgtctacgg tggctcttat tgttaatgaa ctggttcaaa
     6781 acagcttaaa atatgcattt aaggaccgga aacagggaca gatcaggctg aagatcgaaa
     6841 aaggcgccag ttactcctgg atcacagtgc aggatgatgg ctgcggcttt gacaataaga
     6901 agatttccag ggcaaacagc ggactggggc tccgcctgat cagcagtctg gtacaatcaa
     6961 gcctgaaagg agaattgttt atagaaacag gggaaaatgg aaccagtata agattttctt
     7021 tttccatgcc ccaggctgga taatctaaaa taattctaaa cataaaataa aaatagttta
     7081 aaataaaact tgttaaacta ttgacaaaat aatgtgtgag tgctatgatg tagacaatta
     7141 aagcgatatc tctttaacac tgaaaattca attaaacctg atatgcaacg gcgtatatca
     7201 gtaccacaga aaggcatcga agcccagaaa ctttttacag agtttttggg cttttttatt
     7261 gcttatgatc tgcgtggatt ttcggacgtt acctggagtt gtggcccagt taagggctct
     7321 agggccttta tatgggtctc tcccggcggc ggttttacaa ctgccggaaa aaacttacga
     7381 aaaggagtcg tttcataatg gaaaacaagc aaaataaggg cggcggttta aacaagagct
     7441 tgaaactcat ctttgtttat acagttgcta cgggttcaat ttttacgttc gtaaactagg
     7501 gagtatccta aagtttgtgt aaacctccaa actgatgtaa gataaaatta ctcagtttgg
     7561 aggttatttt tatggcaaga agaaaagaca gcccacaa
//
LOCUS       DBMDUA010000138         7586 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4230_length_7586_cov_2.527321, whole genome shotgun sequence.
ACCESSION   DBMDUA010000138 DBMDUA010000000
VERSION     DBMDUA010000138.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7586)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4230_length_7586_cov_2.527321"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(95..1102)
                     /locus_tag="ACZPCL_10015"
     CDS             complement(95..1102)
                     /locus_tag="ACZPCL_10015"
                     /inference="COORDINATES: protein motif:HMM:NF016777.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1162838.1"
                     /translation="MKSIDVFCHLMPQKYAELAMNESPNKSHMFVRALKMQAMSDMEY
                     RKQVMEGFEGYKQIPNIVSPPVELFAGPDKSPKLAAIGNDEIKKITDSDPDRYCGFIG
                     GIPFNNVSASVEEIKRCKDMGAKGIQIYTHMNGEAIDQEKFWPIYEICEKLDLPILIH
                     PVGGQMVPEFPTEDCSKYELWFLIGWPYQTTVAMSRLAFSGIFEDFPQLKIVTHHVGA
                     MIPMLAGRIENGLKMYGGRTAPELKEKLTKTKMKGNPLDTFKKFYADTASFGSASAIR
                     CGLDFFGKDHLLFASDMPFDPEKGPGYIDRTLKCIDSLGLSAEEKESILYGNAERIFH
                     V"
     gene            complement(1319..2515)
                     /locus_tag="ACZPCL_10020"
     CDS             complement(1319..2515)
                     /locus_tag="ACZPCL_10020"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1162839.1"
                     /translation="MVLSKNMQKLVAGSSTIRKLFEEGIELAKEVGAENVYDFSLGNP
                     AAPVPEEVKKSIYDILENRNANFVHCYMPNAGYPEVREKVAANLNKKYNSSFTKDDII
                     MTVGAAGGINCVLRSLLEPEDEVLCFAPFFGEYNSYVMNFGGNLKVVPADYETFQLNV
                     EEADKYITERTKAVIINNPNNPSGVVYNKETLIRLQKMLEKAEERIGHPIYVLSDEPY
                     RELVYDGCELPFAPDYIKNCIVVYSFSKSLSLSGERIGYMAVSRQIAEYDTMINALVV
                     ANRCIGYINAPSLFQLVVADCLDVKTDVEFYDRNRKLLYESMKDMGFECVKPEGAFYM
                     LVKAPTGDDAEFSAAAKKFNLLIVPATSFGCPGYVRMAYCVSNEMIHRALPALQKLAD
                     HYGLKK"
     gene            2825..4228
                     /gene="citG"
                     /locus_tag="ACZPCL_10025"
     CDS             2825..4228
                     /gene="citG"
                     /locus_tag="ACZPCL_10025"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HIR1162840.1"
                     /translation="MNTDSSQFITGREVAVLDMLNARDRRHAIQQELIRTHNATVISF
                     TLNIPGPIKVFPLGEMTFEEGIRLIDSQLHAWKIPVLEKRTIRDFTGNEQFWSVKGDT
                     LFIKEILCLLEDSLSFGRLFDIDVIRTDETKVSRTELGFPGRKCLLCSNDAFVCSRSR
                     THTVKELLDKACELMKDYFEEKQAKKLSSLSMQALLYEVSATPKPGLVDRNNTGAHKD
                     MDIFTFEASAVSLNHYFEKFALCGMEKEHENGHEPFSRIFARLRSLGIQAEDAMMTAT
                     KGINTHKGLIFSLAILNCSLGYMYANHIPYSPDTLLSINRKLVADVLEDFKDITAETA
                     RTNGERLYALYGMKGARGEALSGYATVLKTALPILKEELKKDRSINDAGVITLLSIIA
                     TSEDTNIVNRSSYEEMKELQARIQTVLKEKAHDTHELLTYAKELDAEMIERNISPGGS
                     ADLLALTYFIYLYEQEL"
     gene            complement(4273..5262)
                     /locus_tag="ACZPCL_10030"
     CDS             complement(4273..5262)
                     /locus_tag="ACZPCL_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1162841.1"
                     /translation="MGVTIKDIAQKAGVSYSTVSRALNGIGSTNGDRRKRILEIAKEM
                     GYVPNQAAIHLKLSRSYVIGLYFSTISKMTSPFVLHDVLTGVYSIVGSKYNIVVKGID
                     MHEAGTLNPTYYDGIIVLSQRTEDRAFMEEVLEKKIPMVVICRKVDIDALNVTTDETK
                     AMETAMDYLIENGHRNICVIEGAPNLDSTRLRHIGWQNAARKHGLDPDIIPTVSGTYR
                     YASGYQGAKLLLEYHPTALLCFNDEMAFGAREAIVEEGLKVPDDVSLIGFDNWDMSGY
                     SSMKLTTVERSMSEIAKEGTRVLLRRIEDGVEDNRRIVLENRLIVRNTVRRLV"
     gene            complement(5315..6865)
                     /locus_tag="ACZPCL_10035"
     CDS             complement(5315..6865)
                     /locus_tag="ACZPCL_10035"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR1162842.1"
                     /translation="MFKNARSATIEIGDEGIFHTKREYEVYVNDAFYQKTDRVITGIY
                     DLQPSSDYKVRIVAEDEQACIEIKTDEEFVTLNVRDFGAKGDGITDDTLFIQSAIMAC
                     PKKGRVLIPEGTYKVTSLFLKDDLNLELAQGAVLSAETDRRKFPIYPGLIQSWDETEE
                     YNLGTWEGNPLPMFSAIITGVNVKHVVIYGKGVIDGNANFDEDNWWYQPKVKRIAFRP
                     RMLFLNHCEDVVVAGIQVQNSPSWNIHPYFSDNLRFIDLKVLNPKVSPNTDGLDPESC
                     KNVEIVGVYFSLGDDCIAVKAGKIYMGAKYKRPCENLMIRQCCMRDGHGSVTIGSEMA
                     GGVINLTVKDCLFINTDRGLRIKTRRGRGKDAIVDGILFDNIQMDQVMTPLVINCFYF
                     CDPDGHIEYVRSKEALPVDERTPQIRSLVFKNIEAKNCHVAGAFFYGLPEKKIQKVVM
                     EHVHITYDENPQPGAPAMLDGIEPCTKVGIFADNIEELIMHDVKVEGQEGEAFILRNI
                     ENMISDPQ"
     gene            complement(6990..>7586)
                     /locus_tag="ACZPCL_10040"
     CDS             complement(6990..>7586)
                     /locus_tag="ACZPCL_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HIR1162843.1"
                     /translation="EHVELPHTTQNTGIDLGIKDLCITSGGKKYENPKIIRKNEKKLE
                     KLQRQLAHKEKKSQNYYKTKKKIALCHEKITNTRKDYLHKISHEIISENQVIVSENLQ
                     IKNMVKNHHLAKSISDVSWYELTRQLEYKAKWNGREYVKIDTFYASSQLCSVCGYQNT
                     ETKNLTVREWICPVCGTNHDRDINAAKNILEEGLRQIA"
BASE COUNT         2004 a         1939 c         1381 g         2262 t
ORIGIN      
        1 ttaaaaagaa ctggttcgtt aatactgaat tgatcattgc tgacatttga ttccctccaa
       61 atcgttatct gaacatttat acatttttgt tcatctacac atgaaaaata cgttctgcgt
      121 ttccataaag gatggactct ttttcttctg cagacagccc cagactgtca atacatttta
      181 aagtcctgtc aatataaccc ggtccctttt ccggatcaaa tggcatatca gaggcaaata
      241 acaggtggtc ttttccaaag aaatccagac cacagcggat tgcggaagca gagccaaaag
      301 aagcagtatc tgcataaaac ttcttgaaag tatctaacgg attgcctttc atttttgtct
      361 ttgtcagctt ttcctttaat tcaggtgctg ttctgccccc atacatctta aggccatttt
      421 caatacggcc tgccagcata gggatcatgg ctcctacatg atgagtaacg atctttaact
      481 gaggaaaatc ctcaaagata ccggaaaacg ccaggcggct catagctacg gttgtctggt
      541 aaggccagcc gatgagaaac cacagttcat atttggagca gtcctctgta ggaaattccg
      601 ggaccatctg tcctcctacc ggatggatca ggatcggaag atccagcttt tcgcagatct
      661 catagatcgg ccagaacttt tcctggtcaa tagcttctcc gttcatatgt gtgtagatct
      721 ggattccctt tgcccccata tccttgcatc gtttgatctc ttccacagac gcagacacat
      781 tgttaaacgg gatacccccg ataaatccac agtaacggtc cggatcgctg tctgtgatct
      841 ttttgatctc atcatttcca atagctgcca gcttcggtga tttgtccggc cctgcaaaca
      901 gctccacagg cggtgataca atattgggga tctgcttata cccctcaaac ccttccatca
      961 cctgctttct gtactccata tcagacatag cctgcatttt cagggcacgt acaaacatgt
     1021 gggatttgtt tggtgattca ttcattgcca gttctgcata cttttgcggc atcaaatggc
     1081 agaaaacatc aattgacttc atactttctt ctccttttcg tttttctaaa gtatagcctt
     1141 tacagcccct gcttttctgg tagaattctg ttgtaaattg ggtgtttcct gtcatattct
     1201 gtctggattt atgcacaaaa aaaactgccg cagaacaaag gtgacttttt catcatcctc
     1261 tgctttgcgg cagttatata ttcacatttt gatcagatat ttacactatt ttttcctgtt
     1321 atttcttcag tccgtaatga tcagccagtt tctgcagtgc aggaagtgct ctgtggatca
     1381 tctcattgga tacgcagtat gccatacgaa cataaccagg acagccaaag ctggttgcag
     1441 gtacgatcag caggttaaac ttcttggctg ctgcggaaaa ctccgcgtca tctcctgtag
     1501 gtgccttaac cagcatataa aatgcgcctt ccggctttac acactcaaag cccatatcct
     1561 tcatagactc atataacagc ttgcggtttc tgtcataaaa ttccacatct gtctttacat
     1621 ccaggcagtc agctacaacc agctggaata aagatggtgc attaatgtaa ccgatgcagc
     1681 ggtttgcaac caccagggca ttgatcatgg tatcatactc tgcgatctgg cggctcacag
     1741 ccatatatcc aatacgctca ccagacaggg acagggactt actgaaggaa taaacgacaa
     1801 tgcagttttt aatatagtcc ggcgcaaatg gaagctcaca gccatcatat accagttcac
     1861 ggtatggctc atcagaaaga acgtaaatag gatgtccgat ccgttcctct gccttttcca
     1921 gcattttctg taaacggatc agcgtttcct tattgtatac aacaccagac ggattgttgg
     1981 ggttgttgat gatcacagcc ttggtacgct ctgtaatata cttatcagct tcctctacat
     2041 tcaactggaa agtctcataa tcagccggaa ccacctttaa attaccgcca aagttcataa
     2101 cataactgtt gtactcacca aaaaacggcg caaagcaaag gacctcatct tccggctcca
     2161 gcagagaacg cagaacacag ttgataccgc ctgcagcacc tacggtcata ataatgtcat
     2221 cttttgtaaa agaagaattg tatttcttat ttaagtttgc tgctactttc tcccttactt
     2281 ccggataacc ggcatttggc atatagcagt gtacaaaatt ggcatttctg ttttcaagaa
     2341 tatcataaat gctcttcttt acctcttccg gtacaggagc ggccgggttt ccaaggctga
     2401 aatcatatac attctcagcg ccgacttcct ttgccagctc aataccttcc tcaaaaagtt
     2461 ttcttattgt agaacttcct gcaaccagtt tctgcatatt ctttgaaaga accatctctc
     2521 tacctcctac agcgatcgtg cctttatcct tttgtgtccg caccggctgt tctttttcat
     2581 taacaaaaat tttaacacat taacgtatcc aacgcaatga ttttagaaaa atttattttg
     2641 ccactgtaaa gtcataaagt aattccgcat ctattttatt ataattcaac atctggcata
     2701 ctgtaaccag ctttcctcaa agccttttat atgctatact cattaataaa ggttcttttt
     2761 atttcagctc aaaaacgcag cttttcagaa cctgcctata ccatgatctg gaggtaaaat
     2821 ccttatgaat acagactcat cccaattcat caccggacgt gaagttgcag tactggatat
     2881 gttaaacgca agagacaggc gccacgccat ccagcaggaa ctgatccgca cccacaatgc
     2941 cacagtcatt tccttcacct taaatattcc cggtcccatc aaagtctttc ctttaggaga
     3001 aatgaccttt gaagaaggca tccgtctcat cgactcccaa cttcacgcct ggaagatccc
     3061 ggtccttgaa aaacggacga tccgcgattt tacaggaaac gaacagttct ggtctgttaa
     3121 aggtgatacc cttttcatca aagagatcct ctgcctttta gaagacagcc tgtcctttgg
     3181 ccgtcttttc gatattgatg tgatccgtac cgatgaaacc aaagtttccc gtacagaact
     3241 tggctttcca ggcagaaaat gccttttatg cagcaacgat gcctttgtat gcagccgttc
     3301 ccgtacccac acagtaaaag aactgttaga taaagcctgc gaactgatga aagactattt
     3361 tgaagaaaaa caggcaaaaa aactgtcttc cctgtccatg caggcccttc tctacgaggt
     3421 aagcgccacc cctaaaccag gactggtaga ccgcaacaat accggcgccc acaaagatat
     3481 ggacatcttc acctttgaag ccagcgccgt ttccttaaac cactactttg agaaattcgc
     3541 cctctgcggc atggaaaaag aacacgaaaa cggccacgaa ccattttccc gtatctttgc
     3601 aagactgcgc tctttaggga tccaggcaga agatgccatg atgaccgcca ccaaaggcat
     3661 caatacacac aaaggactta tcttctccct ggcgatctta aactgctcct taggctatat
     3721 gtatgcaaac catatcccct attccccaga cacgctgctc tccatcaacc ggaaactggt
     3781 agccgatgtc ctggaagatt tcaaagatat tacggcagaa accgcccgca ccaatggcga
     3841 acgcctctac gccctctatg gtatgaaagg agcccggggt gaagcactgt ccggctacgc
     3901 aaccgtatta aaaacagctc tccccatcct gaaagaagag ctgaaaaaag accgttccat
     3961 caatgatgcc ggtgtgatca ccctgctgtc cattattgcg acctccgaag acaccaacat
     4021 cgtaaaccgc tcttcctacg aagaaatgaa agaactgcag gcccgtatcc aaactgtact
     4081 aaaagaaaaa gcccatgata cccatgagct tcttacttat gcaaaagaac tggatgctga
     4141 aatgatagaa cgcaacatca gtccaggcgg cagcgccgac cttcttgcac tgacctactt
     4201 tatttacctt tatgagcagg aactttgatc cttctcatat ataattctca tatataaaaa
     4261 actgtatacc agctacacca gccgtctgac cgtattccgg acgatcagac ggttttccag
     4321 aacgatcctg cggttatcct ctacgccgtc ttcgatccgg cgcaaaagga ccctggtgcc
     4381 ctcttttgca atctcactca tactccgctc aaccgttgtc agtttcatac tgctgtatcc
     4441 agacatatcc cagttatcaa agccgatcaa tgaaacgtca tccggcactt tcagcccttc
     4501 ttctactata gcctctctcg ctccgaaagc catttcatca ttgaagcata aaagagctgt
     4561 gggatggtac tccagaagga gctttgcccc ctgatacccg cttgcgtacc ggtaagtccc
     4621 gctgacagtg ggaatgatat ccggatccag gccatgtttt ctggctgcat tctgccatcc
     4681 aatatggcgc agtcttgtag agtccaaatt cggagcccct tcgatcacac agatatttcg
     4741 atggccgttt tcgatcagat aatccattgc tgtttccatt gccttcgttt catcggttgt
     4801 tacattgagt gcatctatat ccaccttacg gcagatcaca accataggga tctttttctc
     4861 cagaacttcc tccataaatg cccggtcttc cgttctctgg cttaaaacaa tgatcccatc
     4921 ataataggtc ggatttaatg ttcccgcttc gtgcatgtca atacctttga ctacaatatt
     4981 atacttactc cctacaatac tgtatactcc cgtgagaaca tcatggagaa cgaagggtga
     5041 tgtcatcttg ctgatggttg agaaatacag accgatcaca taggatcttg aaagtttcag
     5101 atggatcgcc gcctgattag gaacataccc catctccttg gcaatctcca atatccgctt
     5161 ccgacgatcc ccgtttgtac ttccgatccc atttaatgct ctcgaaaccg tagaataaga
     5221 gacccccgct ttctgcgcaa tatctttgat cgttaccccc atgtctattc ctccgtccca
     5281 ttttgatatc ctttacaaac tagtatatca ctttttattg cggatcactg atcatgtttt
     5341 caatatttct cagtatgaac gcctctccct cctggccttc aacctttaca tcgtgcatta
     5401 tcagttcttc aatattatct gcaaatattc ctacttttgt acatggctca attccgtcaa
     5461 gcatggccgg agctcctggc tgcgggtttt catcataggt aatatgaaca tgttccataa
     5521 ccaccttctg gatttttttc tccggaagtc cgtagaagaa agcacctgca acatgacagt
     5581 tttttgcctc aatatttttg aatacaagac tgcggatctg gggagtacgc tcatcaaccg
     5641 gaagtgcctc cttgctgcga acatactcaa tatgtccatc tggatcacag aaatagaagc
     5701 agttaataac gagcggtgtc ataacctgat ccatctggat attgtcaaac aggatcccgt
     5761 caacaatagc atcttttccc cggccacgtc tcgtcttgat cctaagtcca cggtctgtat
     5821 tgataaagag acagtcttta acagtaaggt ttatcactcc tcctgccatt tcacttccga
     5881 tcgttacaga accatgaccg tcccgcatac agcactgacg gatcataagg ttttcacatg
     5941 gacgtttata ctttgcaccc atataaattt ttccggcttt aacagcaata cagtcatctc
     6001 ccagagaaaa atatacacca acgatctcta catttttgca tgattccgga tccaggccat
     6061 ctgtgtttgg ggaaactttt ggatttagta ccttaagatc tataaagcgg agattgtcgg
     6121 aaaaatatgg gtggatattc cagcttggac tgttttgtac ctggataccg gcaacaacta
     6181 catcctcgca gtgattgaga aagagcattc tcgggcggaa tgcgatccgc ttcacttttg
     6241 gctgatacca ccagttgtcc tcatcaaaat ttgcgtttcc gtcaatcact ccttttccgt
     6301 agatcacaac atgtttcaca ttaacacctg taatgatcgc ggaaaacata ggaagaggat
     6361 ttccctccca agttccaaga ttatactctt ctgtctcatc ccagctctgg atcagtcccg
     6421 gatagattgg aaatttcctg cggtctgttt cagcagaaag cacagctccc tgtgccagtt
     6481 ccaaattcag gtcatccttt aaaaacaggc tggtcacctt ataagttccc tcagggatca
     6541 gcacacggcc ttttttcggg catgccataa tggcagactg gataaaaaga gtatcatccg
     6601 tgattccatc tccctttgca ccaaaatccc tgacattaag tgtcacaaat tcctcatcag
     6661 tctttatctc gatacacgcc tgctcatctt ctgccactat acgtacctta taatcggaag
     6721 aaggctgcag atcatagatc ccggtaatca cccggtctgt cttctggtaa aatgcgtcat
     6781 tgacatagac ttcatattct cgttttgtgt gaaagatccc ctcatccccg atctcaatgg
     6841 tagcagatct tgcattctta aaaatacagc ttactttcgt gaactaccca ttgtctaaag
     6901 ccaatgggct tcctgcttcc ctgacctcgt aacctactat ctccacaggc gttaattcgg
     6961 gcagttcctg ccctatgttg ttattccttt tatgctattt gtcttaatcc ttcttccagt
     7021 atattcttcg ccgcattgat atccctgtca tgattcgttc cacagacggg acatatccat
     7081 tcccttactg tcagattttt cgtctctgta ttttggtatc cacagactga acacaactga
     7141 ctactggcat agaaggtatc tattttgaca tattcccttc cattccattt tgccttgtat
     7201 tccaactgcc ttgtcagctc ataccatgat acatcactta ttgactttgc cagatgatga
     7261 ttttttacca tattctttat ctgcaaattc tccgagacta tcacttggtt ttcgctgata
     7321 atctcatggg atatcttgtg aagataatct tttctggtat ttgttatttt ctcatggcat
     7381 aatgctatct ttttctttgt tttgtagtaa ttttgacttt ttttctcttt atgggctaac
     7441 tgcctttgca gtttttccag tttcttctcg ttttttctga taattttggg attttcgtat
     7501 ttctttccac cagaagtaat acataaatcc ttaataccta aatcgattcc tgtattttgg
     7561 gttgtatgtg gcagttccac atgttc
//
LOCUS       DBMDUA010000139         7553 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4245_length_7553_cov_2.317633, whole genome shotgun sequence.
ACCESSION   DBMDUA010000139 DBMDUA010000000
VERSION     DBMDUA010000139.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7553)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4245_length_7553_cov_2.317633"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="ACZPCL_10045"
     CDS             <1..898
                     /locus_tag="ACZPCL_10045"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR1162844.1"
                     /translation="RENMDIYGYRFGKGEKAACIVGAMRGNEIQQMYIGALLVKSLKE
                     LEERGSIVNGNEILVIPHVNDYSINIGKRFWPGDNTDINRMFPGNEEGETTKRIAAGL
                     FEKVKGYSYGIQFPSFYMPGDFVPHVRMMEAGMESASLANLFGLPYVVMRSPRPYDKA
                     TLNYNWQQWNTHAFSIYTGACDQVDESSAKQAVSAVLRFLTRMGILRYTSHSGYIASV
                     VVEQDLTSVCSDNAGIYRRYFKPGDEVKRGEVLADVISPDDGSVISQIHSPTEGTIFF
                     ACGQPLIMEGTLLYRIIRRLHQ"
     gene            932..1483
                     /locus_tag="ACZPCL_10050"
     CDS             932..1483
                     /locus_tag="ACZPCL_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 domain-containing protein"
                     /protein_id="HIR1162845.1"
                     /translation="MEVNFYEMYLEEMALIPVLEAEEEEKVLALAATGDKEAKKRLVE
                     GSLAKVAQMVKEYEGRELPMSDVVQEANVALTAAAAAYDGSQQWQEFLAQEIRQAVEA
                     ALEEQKTEAEIEENMTARVNVLQTVSQVMAKELGREATVAELAEKMKMTEDEVKDIMK
                     LALDAITVNGEGAVSEEQQKEEE"
     gene            1485..2087
                     /locus_tag="ACZPCL_10055"
     CDS             1485..2087
                     /locus_tag="ACZPCL_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR1162846.1"
                     /translation="MKLYIIRHGQTDWNIAKKIQGRQDIPLNERGHFQAQCLGKAMEN
                     RPITAVFSSPQIRAMETAIAVASPAGVPVIPVRDLMEINYGVWEGKTEEELLRDDRAL
                     YEAWWSHPAETAPPEGESINQVNERCRQAWKEIKPQLTGDAAIVAHGGLLAHFMEQLL
                     GSESIAASTVAHNASITTIEYEPETERFVLVEFDDYKHLL"
     gene            complement(2212..4194)
                     /locus_tag="ACZPCL_10060"
     CDS             complement(2212..4194)
                     /locus_tag="ACZPCL_10060"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR1162847.1"
                     /translation="MKELLFLVPVVGLAGLLFAIFLKSYVIKQDPGTDRMREIADAIA
                     EGARAFLASEYRILVIFVAVLFVVIGVGTRNWVTAVCFLTGSLFSTAAGYLGMSAAIR
                     ANCRTANAARTQGMKRALALAFSGGSVMGMAVVGLGLLGVGTLYIITGDVSVLSGFSL
                     GASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDVAG
                     MGADLFESYVGSLISALTLGIVFYQEAGVLFPLLLSAAGIIASMIGSALVKAIGNADP
                     HKALKTGEYSATALVVISALVLSRTFFGNFLAAFTVIAGLFVGVLIGAITEVYTSGDY
                     RFVKKIAKQSETGSATTIISGLAVGMQSTAVPILLVSAGILVANRLMGLYGIALAAVG
                     MLSTTGITVAIDAYGPIADNAGGIAEMSELDDSVREITDKLDSVGNTTAAIGKGFAIG
                     SAALTALALFVSYAEAVQLKTIDILNAHVIVGLFMGGMLTFLFSAMTMESVSKAAHEM
                     IEEVRRQFHEDAGILKGTSRPDYASCVSISTNAALKEMFLPGLMAVLAPIAVGLILGP
                     DALGGLLTGALLTGVLMAIFMSNSGGAWDNAKKYIEEGNHGGKGSTAHKAAVVGDTVG
                     DPFKDTSGPSINILIKLMTIVSLVFAPLFLQFGGLI"
     gene            4433..5680
                     /locus_tag="ACZPCL_10065"
     CDS             4433..5680
                     /locus_tag="ACZPCL_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1162848.1"
                     /translation="MNGIRGNVIVGQSGGPTAVINSSLAGVFKTAKDRGANKVYGMLH
                     GIQGLLQERYVDLEDHIKNDLDIELLKRTPSAYLGSCRFKLPDISQDREIYDKIFDIL
                     NKLEIEYFFYIGGNDSMDTIKKLSDYSLLNGSNIRFMGVPKTIDNDLAATDHTPGYGS
                     AAKYIGSITKEVIRDGLVYDQQNVTILEIMGRNAGWLTGAAALAKCEDCEGPDMIFLP
                     EVTFDVDEFMKKVAELHKKKKSVVVAVSEGVKTADGRYVCELTDGIDFVDAFGHKQLT
                     GTARFLAEKINREVGCKTRAIEFNSLQRCASHIVSRVDITEAYQVGGAAVKEAFEGET
                     GKMIILKRVSDDPYICVTDIYDVHKVANVEKKVPRNWINEAGDYVTEEFVNYIRPLIQ
                     AELTPIMTDGLPRHLYYTDVEKK"
     gene            complement(5839..7317)
                     /locus_tag="ACZPCL_10070"
     CDS             complement(5839..7317)
                     /locus_tag="ACZPCL_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HIR1162849.1"
                     /translation="MKIGFDNDKYLSMQSEHIKERIGQFGDKLYLEFGGKLFDDYHAS
                     RVLPGFAPDSKLRMLLQLADQAEVVIVISAADIEKSKVRHDLGITYDVDVLRLIQEFQ
                     GKGLYVGSVVITQYSGQSGADQFKVKLEHMGIRVYRHYCIEGYPSNIPLIVSDEGYGK
                     NDYIETSRPLVVITAPGPGSGKMATCLSQLYHENKRGIKAGYAKFETFPIWNLPLKHP
                     VNLAYEAATADLNDVNMIDPFHLEAYGKTTVNYNRDVDIFPVLNAIFEGIYGSSPYKS
                     PTDMGVNMAGFCICDDEVCCEASKQEIIRRYYQALNRLAKEDDNSDEVYKLKLLMNQA
                     KITPEMRSVVGPCLKRAEELSCPAAALELDDGRIVTGKTSDLLGASAAVLLNAIKVLG
                     DIPDDVHLIAPSAIEPIQVLKTGYLGSKNPRLHTDEVLIALSMSAATDETAKKALAQL
                     PKLRGCQVHTSVMLSNVDIKTFKKLGVQLTSEPVYEHNKLYH"
BASE COUNT         2294 a         1549 c         1798 g         1912 t
ORIGIN      
        1 cagggaaaat atggacatat acggatatcg ctttggaaaa ggtgaaaaag cagcctgtat
       61 tgtaggggct atgcgtggca atgagatcca gcagatgtat ataggtgctc tgctggtgaa
      121 aagtttaaaa gaattagaag aacggggatc gatcgtaaat ggcaatgaga tccttgtgat
      181 tccccatgta aatgactatt ctattaatat aggaaagcgc ttctggccgg gagataatac
      241 ggatattaac cgtatgtttc cgggaaacga agaaggagag acaacgaaac ggattgctgc
      301 aggacttttt gaaaaagtaa aaggctacag ttacggtatc cagtttccca gcttttatat
      361 gcccggagat tttgttcccc atgtgcgtat gatggaagct ggaatggaaa gtgccagtct
      421 ggcgaatctg tttggactgc cttatgtagt tatgcgaagt ccgcgtcctt atgataaggc
      481 aacccttaat tataactggc agcagtggaa cacccatgcg ttttccattt atacaggagc
      541 ctgtgaccag gtagatgaaa gttctgcaaa acaggctgta tcggctgttt tacgttttct
      601 tacccgtatg gggattttac gttacaccag tcacagcggt tatattgcca gtgtagtggt
      661 ggagcaggat cttaccagtg tctgctctga taatgcagga atttacaggc gttattttaa
      721 gccgggagat gaagtaaagc gtggggaggt gctggcagat gtgatcagtc cggatgacgg
      781 ttccgtgatc agccagatcc attcgcctac agaaggaacc atattttttg cctgtggaca
      841 gcctttgatc atggaaggaa ccctgcttta caggatcatc cgcagacttc atcaatagat
      901 ttaatttaga acaggagaaa accggaaaat tatggaagta aatttttatg aaatgtattt
      961 agaagaaatg gcattgatcc ctgtacttga ggcagaggaa gaagaaaagg ttttagctct
     1021 tgcagctaca ggggataaag aggcaaagaa acgcctggta gaaggctctc tggcaaaagt
     1081 tgcccagatg gtaaaagaat acgagggaag agagcttcct atgtctgatg tggtacagga
     1141 ggcaaatgtg gcccttacag cagctgcagc agcttatgac ggaagtcagc agtggcagga
     1201 atttttagcc caggagatcc gtcaggcagt agaggcagct ttagaggaac agaagacaga
     1261 agctgagatc gaagaaaata tgacagccag agtaaatgta ctccagaccg tatcccaggt
     1321 gatggcaaaa gagctgggaa gagaagcaac tgtggcagaa ctggctgaaa agatgaaaat
     1381 gacagaagat gaagtaaaag atattatgaa actggctctg gatgcgatca ctgtaaacgg
     1441 agaaggtgca gtatcggaag agcagcagaa agaggaagag taaaatgaaa ctatacatca
     1501 tacgccatgg tcagacagac tggaatatag ccaagaagat ccagggacgg caggatatcc
     1561 ccttaaatga aagaggacat tttcaggcac agtgccttgg gaaagcaatg gaaaaccgtc
     1621 ctattacagc tgtattttcc agtccccaga tccgtgccat ggaaacagca atagcagttg
     1681 cttctcctgc aggtgttccg gtgatccctg taagggatct gatggagatc aattacggag
     1741 tctgggaagg aaagacagag gaagaacttt taagagatga cagagccctg tatgaggcat
     1801 ggtggtccca tccggcagaa acagcgcctc ctgagggaga gagcattaac caggtcaatg
     1861 agcgctgccg tcaggcatgg aaggagatca aaccccagct tacaggtgat gccgcgattg
     1921 tggctcatgg cggtctgctg gctcatttta tggagcagct tttaggcagc gaaagcattg
     1981 ctgcatccac cgttgcccac aacgcaagta ttaccaccat tgaatacgag ccggaaacag
     2041 aacgttttgt gctggtagag tttgatgatt ataagcattt gctgtaaaac agatgatgtg
     2101 gagaaattca gaaaaaagga ccttgcagag atcattccct gcagggtcct ttttctaaaa
     2161 atgggtttgg ctgttttaaa cattttcaat cagttatata ggatctatgt attagatcag
     2221 tcctccaaac tgaaggaaga gaggagcgaa aactaaagat acgatggtca tcagcttgat
     2281 caggatgttg atagaaggtc ctgaagtatc cttaaatgga tcacctactg tatcacctac
     2341 aacagcagct ttatgagcgg tacttccctt gccgccgtga tttccttctt cgatgtattt
     2401 ctttgcatta tcccaggcac cgccggagtt ggacatgaag atggccataa gtacaccggt
     2461 taaaagagca ccggttaaca gtccgcctaa agcgtcaggt ccaaggatca gacctactgc
     2521 gatcggagca aggactgcca tcagtcctgg aaggaacatt tcctttaagg ctgcattggt
     2581 ggaaatagaa acacaggaag cataatccgg acgggaagtt ccttttaaga taccggcgtc
     2641 ttcatggaac tgacggcgta cttcctcgat catctcatgt gctgccttgg atacagattc
     2701 catggtcata gcagagaaga ggaaagtaag cataccgccc ataaataaac ctacaataac
     2761 atgtgcattt aaaatatcaa tggtctttaa ctgaacagcc tctgcatagg aaacgaagag
     2821 tgccagagca gtcagagctg cggaaccgat agcgaagcct ttaccgatcg cagcagtggt
     2881 gttacctacg gagtctaact tgtcagtgat ctcacggacg gagtcatcca gttcggacat
     2941 ttcagcgata ccgccggcgt tatcagcgat aggaccgtat gcatcaatag ctacggtgat
     3001 accggttgtg gaaagcatac caacagcagc aagagcgata ccgtaaagtc ccattaaacg
     3061 gttggcaacc aggatacctg cggaaactaa caggatcgga acagcagtag actgcatacc
     3121 aacagcaaga ccgctgatga tcgtagtagc ggaacctgtc tcagactgtt ttgcgatctt
     3181 ctttacaaaa cggtagtcac cagaggtata aacttctgta atagcaccaa taagaacacc
     3241 tacaaaaagt ccggcgatca cggtgaaagc agctaagaag ttgccaaaga aagtacggct
     3301 taataccaga gcactgatca caaccagagc agtagcgctg tattcgcctg tttttaatgc
     3361 cttgtgtgga tcagcattgc caatcgcctt tactaaagca gaaccgatca tagaagcgat
     3421 gatacctgct gcagaaagta aaagcgggaa gagaacacct gcttcctgat agaaaacaat
     3481 accaagagtc agagctgaga tcagggaacc tacatatgac tcaaaaaggt cagcgcccat
     3541 acctgcaacg tcaccaacat tatcaccaac attgtctgcg ataacagcag ggttacgtgg
     3601 gtcatcttcc ggaatgccgg cttctacctt accaaccagg tcggcgccta cgtcagcagc
     3661 ttttgtatag ataccgccgc ctacacgggc aaacagggcg atagaagaag cacctaagct
     3721 gaaaccggag agaacggaaa catcaccggt aatgatataa agggtgccaa cgcctaaaag
     3781 gccaagaccg acaacggcca tacccataac acttccgccg gaaaatgcca gagccagggc
     3841 gcgtttcata ccctgggtac gggcagcgtt tgcagtacgg cagtttgcgc ggatcgcagc
     3901 gctcattccc agataaccgg cagcagtaga gaaaaggctt ccggtgagga aacatacagc
     3961 tgttacccag ttgcgggtgc caacacctat aacgacaaat aatacagcaa cgaaaatgac
     4021 cagaatgcgg tattcagaag caaggaatgc tctggctcct tctgcgatcg cgtctgcgat
     4081 ttcacgcatc ctgtcagtgc ccggatcctg tttgatcaca taggacttaa gaaaaatggc
     4141 aaacagtaag cctgccagtc ctacgaccgg gactaagaat aatagttctt tcataatacc
     4201 ctcctaataa aaatatattt gttaaaaatg tagctaacac cctgtaaata ttgtagcaaa
     4261 tatagttaaa attatcaaca aaaaaattgt aaaaacctaa aaaaaatttg catttaaaat
     4321 tacttgctat tctccctatc ctgtattaca ataaaaaata taagtgtaag cgcttacaat
     4381 gtacgttcga aaaatttgag ggtacatatt aaaacatggg aggtatttta agatgaatgg
     4441 tataagaggc aatgtgatcg taggacagtc cggcggccct acagcagtaa tcaattcaag
     4501 tcttgcaggt gtatttaaaa cagcaaaaga cagaggggca aataaggtgt acggtatgct
     4561 ccacggtatc cagggacttt tacaggagag atatgtggat ttagaggacc acattaaaaa
     4621 tgacctggac attgaacttt taaagagaac accttccgct tatttaggtt cctgtcgttt
     4681 caaactgccg gatatttctc aggacagaga aatctatgac aagatttttg acattttaaa
     4741 taaattggag atcgagtatt tcttctatat cggaggaaat gactccatgg ataccattaa
     4801 aaagctttct gattattctt tattaaatgg aagcaacatc cgttttatgg gtgttccaaa
     4861 gaccattgat aatgacctgg cagctactga tcatacacct ggttacggaa gtgctgcaaa
     4921 atacatcggt tccattacaa aggaagtcat ccgtgatggt ctggtatatg atcagcagaa
     4981 tgttactatc ttagagatca tgggacgtaa tgcaggctgg ctgaccggtg ctgcagcttt
     5041 agcaaaatgc gaagactgcg aaggccctga catgatcttt ctgccagagg ttacttttga
     5101 tgtagatgaa tttatgaaaa aggtagcaga gcttcacaag aagaaaaagt ctgtagtagt
     5161 agctgtatct gaaggtgtga agacggcaga cggcagatat gtatgtgaat taacagacgg
     5221 tattgatttc gtagatgctt tcggtcacaa gcagctgact ggaactgcac gtttcctggc
     5281 tgagaagatc aaccgtgaag taggctgcaa gaccagagca atagaattta actcccttca
     5341 gagatgcgct tcccatattg tatccagagt ggatatcact gaggcttacc aggttggcgg
     5401 cgctgctgta aaagaagcgt ttgagggtga aacaggtaag atgatcatct taaagagagt
     5461 ttctgatgat ccatatatct gcgttactga tatttatgat gtacataagg tcgcaaatgt
     5521 ggagaaaaag gttccaagaa actggatcaa tgaagcaggc gattatgtaa cagaagaatt
     5581 tgtaaattat atccgtccgc tgatccaggc agaattaacc ccgatcatga cagacggact
     5641 tccaagacac ctgtattata cagacgtaga gaagaagtaa gagagtgata aaaaagatcc
     5701 ttcgccagat ggcgggggat ctttttgttg cctgatggaa attatcacat tttttagtaa
     5761 atcacattat attctatttc cataggaatg tcaatatgcc aactgagggc attttaaaca
     5821 atattttatt cttcacaatc aatgatacag cttattatgc tcataaacag gttcgcttgt
     5881 cagctgaaca cccagtttct tgaatgtctt aatatccaca ttagaaagca taacagaagt
     5941 gtgtacctgg cagccgcgca gttttggaag ctgggcaaga gccttctttg cagtctcatc
     6001 cgtagcagcg ctcatggaca gggcaatcag gacttcatct gtgtgaagac gaggattttt
     6061 gctgccaaga taaccagtct ttaatacctg gatcggttca atggcagaag gtgcgatcag
     6121 atgtacatca tccgggatat ctcctaaaac cttgatggca tttaagagaa cagcagcaga
     6181 agcacctaac agatcgctgg tctttcctgt aacgatacgt ccgtcatcta attccagtgc
     6241 agcagcaggg caggaaagtt cttcagcgcg ctttaaacat ggaccaacta cacttctcat
     6301 ctcaggagtg atcttagcct ggttcatcaa cagttttaac ttgtaaactt cgtcagagtt
     6361 gtcatcctct tttgcaaggc ggtttaatgc ctgatagtag cggcggatga tctcctgctt
     6421 ggaagcttcg cagcatacct catcatcaca gatacagaaa ccggccatat ttacgcccat
     6481 atcagtagga gatttgtaag gactggaacc atagatgcct tcaaaaatag catttaatac
     6541 agggaagata tccacgtcac ggttgtagtt gacagtggtc tttccataag cttccaggtg
     6601 gaatgggtcg atcatattga cgtcatttaa gtcagcagta gcagcctcgt aagccaggtt
     6661 taccggatgc tttaaaggaa ggttccagat cgggaaggtc tcaaactttg catatccggc
     6721 cttgatgcca cgtttgtttt catggtagag ctgggataag caggtagcca ttttgccgct
     6781 tcctggtcct ggagcggtaa tgacaactaa tggtctggaa gtttcgatat agtcattttt
     6841 gccgtaacct tcatcactta cgatcagcgg gatattgctt ggatatcctt cgatgcagta
     6901 gtggcggtat acacggatgc ccatgtgctc cagcttcacc ttaaactggt cagcgccact
     6961 ctggccgctg tactgggtga tgactacgct tcctacataa aggccttttc cctggaattc
     7021 ctggatcaga cggagcacat ctacgtcata agtgataccc aggtcgtggc gtaccttgct
     7081 tttttcaata tcagcagcgc tgataacgat gactacttct gcctgatcag ctaactgaag
     7141 cagcatacgc agcttgctgt ccggtgcaaa tcccggaagg actctggatg catggtaatc
     7201 gtcaaaaagc ttgccgccga actccagata cagtttgtca ccgaactgtc cgatccgctc
     7261 tttaatatgc tctgactgca tggaaaggta tttgtcgttg tcgaatccga ttttcatgtt
     7321 cttgtatctc ctgctatgat ttctcttatt taaacacgta catttgcgca tttactttag
     7381 tatagcataa ttttacatgc cataccagaa cttttaaaag ttgtagtttg aaaggttttt
     7441 ataaatataa ggaaagaaat tgtaaaacat tcacagaaca cttataagta acaaatataa
     7501 gaataattaa atatattaat tttactaatt tatataatct gtgctatgat aga
//
LOCUS       DBMDUA010000140         7525 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4260_length_7525_cov_2.377559, whole genome shotgun sequence.
ACCESSION   DBMDUA010000140 DBMDUA010000000
VERSION     DBMDUA010000140.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7525)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7525
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4260_length_7525_cov_2.377559"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>7525
                     /locus_tag="ACZPCL_10075"
     CDS             <1..>7525
                     /locus_tag="ACZPCL_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MSCRAMM family protein"
                     /protein_id="HIR1162850.1"
                     /translation="PWKDWTWDMTPAQAMLMQLGYKEADGEGAPWVSINLDSMNGKAV
                     EQILDWYTEHSFYNMAAVSEIKEEEGQWQAKLFYDYSGNTEAGSAVYDRADGQLYVKK
                     ERENGHYWICIPKGKFRLSSHTAYIKEKWEYVEDELKLIYEPVYERYEEGEVLLDPNG
                     VPIQEMEQVPIFEERETTYVTTKEEPVEAFWDPEKREYHILVENETDWSVEKEEVEET
                     FRAVTGQTTISVNGKEVPYNQYLVSMGAAVDVEAEQEETDPGSFVKYVHLAYPGQNQT
                     VQDGGTEKEPVQVLERAIRQPVRITKHISQSSYENVNTYGSVHNDPVTTFLGLFRKNG
                     SAEGRKLLDQFSFRIYLKQNLLNIYVDDEGKILSEDISSPAYKELVQTIYLPPEDAEN
                     RGHQLMERNEDGSCNYRKFFDAMEGAEKKKGGIYPKEVLTQFALDHYDIRSYKEEILK
                     DRPYMDSDRAYKQALDQARKEAARYLDQFTGLKERLSIEWERDANGGTDGNRKTIQCN
                     QRNGKDDYYEYSIPLPYGEYVIEEQMPSDLEKELANRHYKIEEPREITLPFVPEIIRN
                     EDTGEETVLDEIGSAYFRYHSSDSPDELIRKYKIRFNEEDRKILADGIDGSFAIYPYG
                     LDKDILPEYSTGTISQSEDHVILDGVIYDGSERENGQMEICDQVPGMKGKNTAIEGKF
                     APMLVPWSILTPEYDRINPDTGNIETLQPGGSGAGFNYIAFAQEDFENTYFNSRLRIE
                     KIDSETGENLIHEGALFRIYAAKRDAQKNGINSVTGTGQVLFGPAEDAEGNKVVDADG
                     KEILYPRVGQDNGSQDDLPVKLDQDGVPLYDETQRISQVDETGTERGIFRSFTTLREV
                     LIHGKLEKLPVGYIETPRPLGAGAYVLVEVQAPEGYVKSRPVAFEVYGNEVSYYKENV
                     YRDGTTDGYERKTAANYEYAIPVTGDGNKNAYETVSQIPVENHPSVMRIRKVEDGDSL
                     VGNENGLLKTDALGQEETSGGLEEDIWVNDRGDEIIYQVYGRKEKLEERGDVREIHFD
                     RELGTWTGKVTKKMDFCSEEIIEGTEKELKAMEGVKLLYDRKGEFTGKGIRFKVTVSG
                     ATLALYQGLWMEKEEDGSYKGVTAEVQDGKTIKITASETGSRRQILETGKDAGPAGLA
                     IWQDEKVENQPEDLYFYDLDQLNEEGRLERTEDKSVYKVLDERGNFICYADVKTGMAF
                     VYDDHERIMAYIADETGEKKLVYSVQVYKDKNGESVYKEKRSVDDENGLPVYDKQGHF
                     TTKEERWTSDNSTDSKGEAERRGGLHEIKRLPFGAYILQEEQVPFEQGYVQAPYQGIL
                     FKDSIEPQEYFHSNAFTRAAFAKVDTRTQEEIKGAVMTFYEAATDEAGRILTDEDGIY
                     LPGKKYASWVSGYACDDQGNVKTDEEGNRIETKKPHWIDHIPVGEYVLEETECPYLQG
                     YVQRKRQNIQILETEHVQSFTMEDDFTAAEIRKEDGKTGELLYEDSLAKLVLYQVPEG
                     EEDKPLADLMGEDNKLLDFERAGFKEWMEIWATGREEADAAGLNPIAKYDHRWENVPG
                     TLKGRYCFTEEGTVRFEYLPVGSYILAEKETPSGYATADPILFEITDTGHLEKIHQVV
                     MKDEPLSLVVSKNTITGGKEVAGAKLGIYPVDEEGTVSEKPLLLHIPEEEGRYRDEEA
                     IWFSGTDGVYTEEENENGELPEGFEVGDLKPHLIEYIPVGSYILREEMTPYGFLQSVD
                     IPFVIEDTREIQKKEMQDEIPEGGLTVIKHDADDADQVLEGAEFELFNQTLDISCEKQ
                     VTDEKGKAQFSSQPIGYLDQEGKFSPYDYLVKETKAAPGHMLSEKTLEFQFEYKDEKT
                     PLIELTYDPVNDSNRVLVKKLLRDTEEYLEGAVLRLEREEKVILEPDERGDQGESQKK
                     DQKKYQAKDQMKEEKTWVVVETWITGKQPHLIKGLTAGDYRLVEEKAPTGFTKSEEPV
                     YFTITDGMTEIPEITLRNYGTIISVEKREASSEKLLSGAKLELVCKDTMEVISQWISD
                     QENGQVFNGLEPGTYIIREIEAPAGYEKDKEMEIRILDQAETVQEFVFYNSRIRSSGG
                     GGSHTVPKKLYISFKKTDEKGEPLAGAVFAFYDQTGRLAKIAESGPDGMFKIICPPNG
                     TYIFKEIKAPDGYELSQELYHFTVEKDQNVKGVFQIKNEKIQKKTKGRIQAFYQVKGR
                     NGNFKGLNGKYSGSSVKTGDNSQIGWILLVTCVCLAGAGWCFSDSSRKRKKMVLFLGL
                     VMGAVSLFAFSAMAQEQDASLYDESFYASQEIIYKKMEGVDTVPETAWVQVKDRTTGR
                     KTKRILPLEQFSRFNEHWEDGLEITIEQEKEFQNEITEAEILDEAGLPASDYEITQIE
                     KGEDGKLVARGRKKVFDCRAVYSGLIAEKDWDRGEMPDTDLKREWEQDVELKPAVTGS
                     KFVGKMVLAVVTGSCVGIGAYWILKKGKLNKASLKIREKRRILGIILLTGAAAGIVYT
                     GNTVRKYRLAAEKYESIRREVQKTETSY"
BASE COUNT         2522 a         1270 c         2056 g         1677 t
ORIGIN      
        1 tccctggaag gactggacct gggatatgac accggcacag gcaatgctta tgcagttagg
       61 ctataaagaa gctgacgggg aaggggcacc atgggtttct ataaatctgg attctatgaa
      121 tggaaaggcc gtagaacaga tccttgactg gtatacagaa cacagttttt ataatatggc
      181 cgccgtttct gagataaagg aagaagaggg acagtggcag gcaaaacttt tttatgacta
      241 ttctggaaat acagaagcag gatctgcagt ttatgaccgg gcagacggac agctttatgt
      301 gaaaaaagaa agggaaaatg gtcattactg gatctgcatt cccaaaggaa aattccgttt
      361 aagcagtcat accgcttaca taaaagaaaa atgggaatat gtggaagatg agcttaaatt
      421 aatttatgag cctgtttatg aacggtatga agaaggagag gttcttttag atccaaatgg
      481 agtaccgatc caggaaatgg aacaggtccc catttttgaa gaacgggaaa ccacgtatgt
      541 gactacgaaa gaggagccgg tagaggcttt ctgggatcct gaaaaaagag agtatcatat
      601 tttggtggaa aatgaaacag actggtccgt ggaaaaagaa gaggtagaag aaacgttccg
      661 tgcagtgacc ggacaaacca ccatttctgt aaatggaaaa gaggttcctt ataatcagta
      721 tctggtatcc atgggagcag ctgtggatgt agaagcagaa caggaagaaa cagatcctgg
      781 aagttttgta aaatatgttc atctggcata tcctggtcag aaccagacag ttcaggatgg
      841 tggaacagaa aaagaaccgg ttcaggtttt ggaacgggcg atccggcagc cggtacgcat
      901 taccaaacac atttcccagt cttcttatga aaatgtaaat acctacggtt ctgtgcataa
      961 tgatccagtc actacctttt taggcttatt ccgtaaaaat ggatctgcag aaggccggaa
     1021 acttttagat cagttttcat tcaggatcta cctgaagcaa aatcttttaa acatttatgt
     1081 ggatgatgag ggaaagatcc tgtcagaaga tatcagttca ccagcttata aggagctggt
     1141 acagactatt tatctgccgc cggaagatgc agaaaacagg ggtcatcagc tgatggaaag
     1201 aaatgaggat ggcagctgca attaccggaa attttttgac gcaatggaag gcgcagaaaa
     1261 gaagaagggc ggtatttatc ccaaagaggt actgacccag tttgccctgg atcattatga
     1321 tataagatcc tataaggaag agatcttaaa agaccggcca tatatggaca gtgacagggc
     1381 atacaaacag gctttagacc aggcgagaaa agaagcagcc cgttatctgg atcagtttac
     1441 agggttaaaa gagcgtctgt ctattgaatg ggaacgggat gcaaatggcg gtacggatgg
     1501 taacaggaag accatccagt gtaaccagag aaatggaaaa gacgattatt atgagtattc
     1561 cattcctctc ccgtatggtg aatatgtgat cgaagagcag atgccttctg atctggaaaa
     1621 agaactggca aacaggcatt ataagattga ggaaccaagg gagatcacac tgccctttgt
     1681 gccggagatc atacgaaatg aggataccgg ggaagaaacg gttttagatg agatcggcag
     1741 tgcctatttt cgttatcaca gtagtgacag tccggatgaa ctgatccgca aatacaagat
     1801 ccgctttaat gaagaggacc ggaagatcct ggcagatggt atagatggtt cctttgccat
     1861 ttatccttat gggcttgata aagatatttt gccggaatac agtacgggaa ctatttcaca
     1921 aagtgaggat catgtaattt tagatggtgt gatctatgac ggaagtgaac gggaaaatgg
     1981 ccagatggaa atatgtgacc aggttcctgg tatgaagggg aaaaatactg ccattgaggg
     2041 caaatttgcg ccgatgctgg taccgtggag catcctgaca ccggaatatg accgaatcaa
     2101 cccggatacg ggaaatatag aaaccttaca gcctggggga agcggtgccg gatttaatta
     2161 tattgcattt gcccaggaag attttgaaaa tacctatttt aattcaaggc tgcggatcga
     2221 aaagatcgac agtgaaacag gggaaaatct gatccatgag ggagctttat tccgcattta
     2281 tgctgcaaag cgggatgcac agaaaaacgg gataaacagt gtgaccggaa ccggacaggt
     2341 gctgtttgga ccggcagagg atgcagaagg aaataaggtt gtggacgcag atggaaagga
     2401 gatcctttat ccgagagtgg ggcaggacaa tggttcacag gatgatcttc ctgtgaagtt
     2461 agaccaggat ggggtccctc tttatgatga aacccagcgg atcagccagg tggatgaaac
     2521 cggcacagag agagggatat tccgctcttt tactacactg cgggaagtct tgatccatgg
     2581 aaagctggaa aaactccctg tgggatacat tgaaacgccc agacctttag gggcaggtgc
     2641 ctatgtcctg gtagaagtac aggcaccgga aggttacgta aaaagccgtc cggttgcttt
     2701 tgaagtttat gggaacgaag tatcctatta taaggaaaac gtgtacagag acggtacaac
     2761 agatggatat gaacggaaaa cagctgcaaa ctatgagtat gccattccgg taacgggaga
     2821 tggaaataaa aatgcttacg aaacagtcag ccagatcccg gtggaaaatc atccttctgt
     2881 aatgcggatc cggaaagtag aggatggcga ttccctggtt ggaaatgaaa atggcctgtt
     2941 aaaaacggat gctttaggcc aggaagaaac aagcggcggc ctggaagaag atatatgggt
     3001 aaatgacaga ggagatgaaa tcatttacca ggtatatgga agaaaggaga agttagaaga
     3061 aagaggagat gtaagagaga tccattttga ccgggaactg gggacatgga ccgggaaagt
     3121 aacaaagaaa atggatttct gttcagaaga gatcatagaa ggtacagaaa aagaactaaa
     3181 agcaatggaa ggagtaaaac tcctttatga ccggaagggg gaatttacag gaaaagggat
     3241 ccgttttaaa gtaacggtct caggagcaac actggcttta taccagggac tctggatgga
     3301 aaaagaagag gacggaagtt ataagggagt aacagctgaa gttcaggatg gtaaaaccat
     3361 aaagatcact gcttctgaaa caggaagcag gcggcagatc ctggagacag gaaaagatgc
     3421 cggtcctgcc gggcttgcta tatggcagga tgagaaggtg gaaaaccagc cggaagatct
     3481 gtatttttat gatctggacc agttaaacga agaagggcgc ctggagagga cagaggacaa
     3541 gtctgtatac aaagtattag atgaacgggg aaacttcatc tgttatgcag acgtaaaaac
     3601 aggaatggcc tttgtatacg atgatcatga acggatcatg gcttacattg cagatgaaac
     3661 gggagaaaag aagctggttt attctgtcca ggtctataag gacaagaatg gggagtctgt
     3721 ttataaggaa aaacgttctg tagatgatga aaatggtctg cctgtttacg ataaacaggg
     3781 gcatttcaca acaaaggagg aacgctggac cagtgacaac agcacagact ccaaagggga
     3841 agcagagcga agaggcggcc ttcatgagat taagcgcctg ccttttgggg cctatatcct
     3901 tcaggaagaa caggttccgt ttgaacaggg ctatgtgcag gcaccttatc agggaattct
     3961 ttttaaagac agcatagagc ctcaggaata tttccattcc aatgccttta caagggcagc
     4021 tttcgccaag gtagacaccc gtacacagga agagatcaaa ggggctgtga tgacgtttta
     4081 tgaggctgcg acagatgaag cgggaaggat attaacagat gaagacggaa tttatctgcc
     4141 tggaaagaaa tatgcttcct gggtcagcgg ttatgcctgt gacgatcaag gcaatgtgaa
     4201 aacagatgag gaaggaaacc ggatcgaaac aaaaaaacct cactggatcg atcatattcc
     4261 ggtaggggag tatgtattag aagaaacaga gtgcccgtat ctgcagggct atgtgcagag
     4321 aaaacggcag aatatccaga ttttggaaac agaacatgta cagtctttta ctatggaaga
     4381 tgattttaca gctgcagaga tccgcaagga ggatggaaag acgggagaac ttctctatga
     4441 agacagtctg gcaaaactgg tcctttacca ggtgccggaa ggagaagaag ataagccgtt
     4501 agcggatctt atgggagaag ataataagtt gttagacttt gaacgggccg gttttaaaga
     4561 atggatggaa atatgggcta caggaagaga agaagcagac gctgccggct taaatcccat
     4621 tgcaaaatac gatcatcggt gggagaatgt gccgggtacg cttaaaggca ggtattgctt
     4681 tacagaagaa ggaacggtac ggtttgaata tcttcctgtg ggatcttaca ttcttgcaga
     4741 aaaagaaacg ccttccggtt atgccacagc ggatccaatt ttatttgaga tcacagatac
     4801 cggtcatttg gaaaaaatcc atcaggtggt tatgaaagat gaacctttga gtcttgtggt
     4861 cagcaagaat acgattaccg gaggaaaaga agtagcagga gcaaagctgg gaatctatcc
     4921 ggtggatgaa gagggcacag tcagcgaaaa gccgctgctt ttgcatattc cggaggaaga
     4981 agggcgttac agggatgagg aagccatatg gttttccgga actgatggag tgtatacaga
     5041 agaagagaat gaaaatgggg aactgccaga aggatttgaa gtgggagatc tgaaaccaca
     5101 tctgatcgaa tacattcctg ttggttctta tattttaaga gaagaaatga cgccttatgg
     5161 atttttacag tctgtggata tcccatttgt aatagaagat accagagaga tccagaaaaa
     5221 ggagatgcag gatgaaatac cagaaggcgg gctgacggtg atcaaacatg atgcagatga
     5281 tgcagatcag gttttagagg gagcagaatt tgaactcttt aaccagacct tagacatatc
     5341 ctgtgaaaag caagtaacgg atgaaaaagg aaaagctcag ttttcttccc agcccatcgg
     5401 atatctggac caggaaggaa aattcagccc gtatgattat cttgtaaagg aaacaaaagc
     5461 agcgccggga catatgcttt ctgaaaaaac gctggaattc cagtttgaat ataaagatga
     5521 aaaaacacct ttgatagagc ttacttatga cccggtaaat gacagcaacc gtgtactggt
     5581 gaaaaagctt ttaagggata cagaagagta tctggaaggt gctgttttga gactggaacg
     5641 ggaagaaaag gtaattttgg aaccggatga aagaggtgat cagggcgaaa gtcagaagaa
     5701 agatcagaaa aaatatcagg cgaaagatca gatgaaagag gagaagacct gggtagtagt
     5761 agaaacatgg atcacaggaa agcagcccca tctgatcaaa ggacttacag ccggagatta
     5821 cagactggtg gaagaaaaag caccgactgg atttacgaaa tcagaagaac cggtgtattt
     5881 taccattaca gatggaatga cagaaatacc ggagattaca ctgcgcaatt atggcaccat
     5941 tatatccgta gaaaaaagag aagccagttc agaaaaactt ctttccggcg caaagctgga
     6001 actggtatgc aaagatacca tggaagtgat cagccaatgg atatcagacc aggaaaatgg
     6061 tcaggtattt aacggtctgg aaccgggaac ttatatcatc agggaaatag aagcaccggc
     6121 cggatatgag aaggacaaag aaatggagat ccggatctta gaccaggcag aaacagtgca
     6181 ggagtttgtt ttctataaca gccggatccg ttcatctggc ggaggcggaa gtcatacagt
     6241 gccaaagaaa ctgtatattt cctttaaaaa gacagatgaa aagggagaac ctcttgcagg
     6301 agctgtgttt gcattttatg accagacagg acgattggca aagatagcag aaagtggccc
     6361 agatggaatg ttcaagatca tttgcccgcc aaatggaact tatattttca aagagataaa
     6421 agcaccggat ggttatgaac tgagtcagga actgtatcat tttacagtag aaaaggatca
     6481 gaatgtaaaa ggtgtatttc agatcaaaaa tgaaaagata cagaaaaaga caaaaggaag
     6541 gatccaggct ttttaccagg taaaaggaag aaatgggaac tttaagggct taaatggaaa
     6601 atattccggc agcagtgtta agacaggaga caacagtcag attggatgga tcctgttggt
     6661 gacatgtgtg tgtctggcag gtgctggctg gtgtttttct gatagcagcc gaaaaagaaa
     6721 aaagatggtt ttgtttctgg gacttgtgat gggggcagtg agcctgtttg cattcagtgc
     6781 aatggcccag gaacaggatg caagtctcta tgatgaaagc ttttatgcat cccaggagat
     6841 catttacaaa aagatggaag gagtggatac agtaccggag actgcctggg tccaggtaaa
     6901 ggaccggaca acgggaagaa aaacaaagcg gatcctgcct ttagaacagt tcagccgttt
     6961 taatgaacac tgggaagatg ggctggagat cacgatagaa caggaaaagg aatttcagaa
     7021 tgagataaca gaagcagaaa tattagacga agcgggttta cccgcttcgg actatgagat
     7081 cacacagatc gagaaaggag aagatggaaa actggttgcc aggggcagaa aaaaggtatt
     7141 tgactgcagg gcagtataca gtgggctgat cgcagagaaa gattgggaca ggggagaaat
     7201 gccggatacg gatctgaaaa gagaatggga acaggatgta gaattaaaac cagctgtcac
     7261 aggatcaaag tttgtaggaa aaatggttct ggcagtggta accggcagct gtgtcggtat
     7321 tggcgcctac tggatcctga aaaagggaaa gttaaataaa gcaagtttaa agatcaggga
     7381 aaagcgccgg atcttaggga taattctttt gacaggagct gcagccggaa tagtctatac
     7441 cggaaataca gtaagaaagt atcgtctggc tgccgaaaaa tacgaaagta tccgcaggga
     7501 ggtacagaaa acagaaactt cttat
//
LOCUS       DBMDUA010000141         7334 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4358_length_7334_cov_2.508088, whole genome shotgun sequence.
ACCESSION   DBMDUA010000141 DBMDUA010000000
VERSION     DBMDUA010000141.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7334)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4358_length_7334_cov_2.508088"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2560)
                     /locus_tag="ACZPCL_10080"
     CDS             complement(<1..2560)
                     /locus_tag="ACZPCL_10080"
                     /inference="COORDINATES: protein motif:HMM:NF021028.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1162851.1"
                     /translation="MRVFDAEIVLSAEVAGAEGVTVKLTALSDVLPENGYIEANAVED
                     EAQLELMKAAADDILKAENRRVTDLFAADITLYNEDGEAVQPDGSVKVTFEGTAIGNS
                     GTRVLYMGENASDTETYDAQMIKSVAADGDATAFMTDHFSLYVTADTEEIICYEVNFY
                     YKDAEGKDVFISGPQYVEDGKAAEAPAVPDRDGYRFTGWDTDFSKVTGDMKVYAGYAP
                     IAGQVRLTVNYVYSNGSLAAQPWVSHVESGVPCNLTAESPAIQGFTPDQTTVSFNDAY
                     TTDKTVTVTYKGAKVNYTVNHYLLNVDDTKPDTPAATETVPGETGLTTEAKAKEYAGF
                     TPRAISQGTVNADGSTVVEILYERNYYTLTWNTGENASYIAPEQVRCGAAVTKPDKNP
                     TKVGYEFKGWENLQETMPAEDLVVTAKWEAAKRADYKIIYWTETVKEGVYTVNHVING
                     NGTVGSSIPNGSYKVPEGYKTTPVAEKTDGDVKITADGTAVKNVYFARATYTITFMRW
                     NSRKWKWKEDESLRITARYGVDVSAKWETACADDGWGPNSSGNIQYTLLANMPAENLT
                     MYEKDAGSGKKIIYYVEGLDGERTTYRTFEASSNVHLTKEDQMSITGFTYSDWKQYNE
                     WYEEPVLWLYYTRNSYNLRFENCQPMDPESIKFEAKLSTGKPSRDPGRPSYVDSDYTF
                     AGWYLDPGFKEPVNWNETMTEDGLTIYAKWEKPKYTVDFVTNCKEEKASITREKGFVL
                     TEEELAAPDKADDEFLGWYTDSALTKKFIPESQIVKDTTLYARWRNSDTVTYTVKYMC
                     GDVAIADAKQQTVIRNSTVSEPAETVEGYYPETLSISAMITKDGQEIIFKYRAVQ"
     gene            complement(2702..4009)
                     /locus_tag="ACZPCL_10085"
     CDS             complement(2702..4009)
                     /locus_tag="ACZPCL_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162852.1"
                     /translation="MRGRSRRKKRTGKMVSLQKRFLAFMLAFAMIFTNVGTDLHVSFA
                     ASGNKVDFTIGGADLVDAIRQAIEDENVVSQDSLDFTDGATEKFEALFFGDGKVYEVY
                     PDIQGDSMEAELRVFVKLPADADDTYMVTGEEEVYFLYVNNGEDTISCSTTVTRTENG
                     KEKEKTTKRITIKSYEDKFGDEERNIISKPAETAPAEKPSEAVNEETTENVVNPTDAE
                     KETTAAEETAEVPETKEDVEESKEQPEETKEEETEAATEAPEEITEPETEEAAETEAA
                     ESETEASEPEAEAPAEEPAQEGEVTASISRHGVPLVAMKEDAENAADGAEPTEPEKEE
                     AAEAAEPVKEEKAEELKKEEASEPETAPEPETETTVEETTTEETTAEKTTAEAEKESR
                     PEGSAAEETTAEAETEPEVPAGPGETTVPEGTPESTEPETEPA"
     gene            4307..5464
                     /locus_tag="ACZPCL_10090"
     CDS             4307..5464
                     /locus_tag="ACZPCL_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1162853.1"
                     /translation="MLNQFVLRNYRLFKNETLLDFFPAPINEHKASLLTAQGDGESFL
                     PVISLYGPNGCGKSSILEALWNVCRLAAGDFSLITSSVRSYCRLDNTCRELPLSFDLL
                     FRRNGFLFRYQLDVKQGAVLEENMFYGKPGSDDAGVLFARKANELHIGNEAGKMDFST
                     LPAGVSLLRYLDPKSSSECAKAAASWFSQVLFFREHDYKKAPDLPSEVEERQVICRLL
                     QAMDIDILDYSITKEQGFDDPSLILTHGKADGNTFFVSFNEESTGIRKLLTLIPLVVK
                     SLRLGSLLLADDLDNVLHPHLLRFILSLYQDHEKNPHHAQLVFTSHNTAILNPAVMRR
                     DEICLCCRPEGTDAVLYPLSSYKKENGLIPRNDEAYGKQYLEGRYGANPRI"
     gene            complement(5440..6627)
                     /locus_tag="ACZPCL_10095"
     CDS             complement(5440..6627)
                     /locus_tag="ACZPCL_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR1162854.1"
                     /translation="MGNDMDKKGILTVRMMGTISVEYEGEFFPVGTALTGKILQLFLI
                     LLYAGKKGVGREELLDVFYGNGEYANPSGSLRAAVFRLRRLLKEMLPEHEYICTDGGI
                     YRWDEGNVTVSIDARNFEKAAQSALATGKKEELCHACSLYYGEFLFQMTGEKWVNVIG
                     VKYQELYFKCLRLASRLLKADREYDRLLNLSTAASKLYPYEECQMMKLDCLIALKRYQ
                     EAMEVYKQVVVQYFEEQGLPPSETMLQRFRLMSGQISYTSDMLKDIENTLKEREETQG
                     PYYSTYPSFIDSYRLVSRMAERFSFEYTLFCITLVDGKGALLEGEMVQTTSEMLKNAI
                     GESLRKGDLFTCYSPVQYLVLLNGAGQEHVLAVCERVERSLKQWENGKKINFSHQILG
                     LAP"
     gene            complement(6684..7187)
                     /locus_tag="ACZPCL_10100"
     CDS             complement(6684..7187)
                     /locus_tag="ACZPCL_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HIR1162855.1"
                     /translation="MYDNLTKDDIKKMQEEIEYRKLVVRKEALEAVKEARAQGDLSEN
                     FEYKAAKQDKNRNESRIRYLEKMIKTARIVSDESKSDEVGLGDRVELYIPEDDETESY
                     KLVTTVRGNSMKGLISIDSPLGKAIKGRKTGDKVYIKVNDTYGYEAEIRSIDKTAKEE
                     EDQLRSY"
BASE COUNT         1658 a         1846 c         1317 g         2513 t
ORIGIN      
        1 tctgtactgc tctatactta aatatgatct cctgtccatc ctttgtgatc attgcactga
       61 tggataaagt ttcaggataa tatccttcta ctgtttcagc aggttcactt actgtggaat
      121 ttcttattac agtctgctgc ttcgcatcgg caatcgctac atcaccacac atatatttca
      181 cagtatatgt gactgtatca gagtttctcc atcttgcata taaagttgta tcctttacga
      241 tctgtgactc tggaataaac ttctttgtaa gtgcgctgtc agtataccag ccgaggaatt
      301 catcatctgc tttgtccggt gctgccagtt cttcttctgt taaaacaaaa cctttttccc
      361 tggtaatact agctttttct tctttacaat tagttacgaa atcaactgta tatttcggtt
      421 tttcccattt tgcgtagatg gtaagtccat cttctgtcat ggtctcattc caatttaccg
      481 gttccttaaa gccaggatcc agataccagc ctgcaaatgt ataatcagaa tctacatatg
      541 aaggtcttcc tggatctcgg gaaggtttgc ctgtggacag ttttgcctca aatttgatgc
      601 tttccggatc cattggctga caattctcaa agcgaagatt ataggaattc ctggtataat
      661 agagccagag aacaggttct tcgtaccatt cattatactg cttccagtca gaatacgtaa
      721 atccagtaat tgacatctga tcttctttag ttaaatgtac attacttgaa gcttcaaatg
      781 tcctgtacgt tgttcgttct ccatctaacc cttccacata gtaaataatc tttttacctg
      841 aacctgcatc tttttcatac atggtcagat tttcagctgg catattcgct aaaagcgtat
      901 actgtatatt tccagaagaa tttggtcccc agccgtcatc tgcacaggcg gtttcccatt
      961 tcgcagatac gtctacacca taacgggcgg tgatccttaa actttcatct tccttccact
     1021 tccacttccg actgttccat ctcataaacg taatggtata cgtagctctt gcaaaataaa
     1081 cattcttaac tgccgttcca tcggccgtta tcttcacatc accatccgtt ttttctgcta
     1141 ctggtgttgt cttatatcct tccggtactt tataacttcc attcgggata gaacttccca
     1201 ctgtaccatt tccatttatc acatggttta cagtgtaaac accctctttt acagtttcag
     1261 tccagtaaat aatcttataa tcagctctct ttgctgcttc ccatttcgca gtaactacca
     1321 gatcttctgc cggcatggtc tcctgaaggt tttcccagcc tttaaattca tagcctacct
     1381 ttgtcggatt cttgtctggt tttgttacgg ctgcaccaca gcgtacctgc tcaggtgcaa
     1441 tataagaagc attttctcct gtattccagg tcagagtata ataatttctt tcatataaaa
     1501 tctctactac cgtacttcca tctgcattta cagttccctg actgatagcc cgtggtgtaa
     1561 atcctgcata ttcttttgct ttggcttcag ttgtcagtcc ggtttcacct ggcactgttt
     1621 ctgtagctgc aggtgtatct ggctttgtat catctacatt taacagataa tgatttactg
     1681 tgtaattaac ttttgcacct ttgtaggtaa cggtcacagt cttgtctgtt gtatatgcat
     1741 cattaaagga tacagttgtc tgatctggtg tgaatccctg gattgcagga gattctgctg
     1801 tcaggttaca gggaacaccg gattctacat gagaaaccca cggctgtgct gccagactgc
     1861 cattgctgta cacataattt acagtaaggc gtacctgacc ggctattggt gcataaccag
     1921 cataaacctt catatcacca gttactttac tgaaatctgt atcccagcct gtaaacctgt
     1981 atccatcacg atccggcact gctggcgctt ctgctgcttt tccatcttcc acatactgcg
     2041 gaccggaaat aaacacatcc tttccttctg catctttata atagaaattt acttcataac
     2101 agatgatctc ttctgtatca gctgttacat acagtgagaa atgatctgtc ataaatgctg
     2161 ttgcatcacc gtctgctgct acagatttta tcatctgtgc atcatatgtt tctgtatcag
     2221 aagcattttc acccatgtaa aggactctgg tgccggaatt tccaatggcg gttccttcaa
     2281 aagtaacttt tacacttcca tctggctgaa ctgcttcacc atcttcattg taaagggtga
     2341 tgtctgctgc aaaaagatcc gttactctgc ggttttcagc ttttaatata tcatctgcag
     2401 ctgctttcat aagctccagc tgagcttcat cctctactgc attagcctca atatagccat
     2461 tttcaggaag cacatcgctt aaagctgtca gcttcactgt cacgccttct gctcctgcaa
     2521 cttctgcaga aagtacaatt tcagcatcaa atacacgcaa tgcattcagt gtactcttat
     2581 aaaccttcgc tgtactgcag taaccaatac ctaccagatc tccgtcatct gctgtttttg
     2641 ccggtgcttc tggctgtggt gctggtaatg tttccgggat agccgctgtt gtttctgccg
     2701 gctatgcagg ctctgtttcc ggttctgtac tttccggggt tccttctggt acagttgtct
     2761 ctcccgggcc tgccggaact tccggttcag tttctgcctc tgctgtggtt tcttctgctg
     2821 cagagccttc cggtctgctt tctttttctg cctctgctgt ggttttttct gctgtagtct
     2881 cttctgtcgt agtctcttct acagttgttt ctgtctctgg ttcaggtgca gtctctggtt
     2941 cagaagcttc ttcctttttc agttcttccg ctttttcttc tttgaccggc tctgcagcct
     3001 ctgctgcttc ttccttttcc ggttctgttg gctctgcccc atctgctgca ttttctgcat
     3061 cttccttcat ggcaactaac ggtacaccat gtctggaaat agaagctgtt acttcacctt
     3121 cctgcgccgg ttcttctgcc ggagcctctg cctctggttc agaagcttct gtctcagatt
     3181 ctgctgcttc cgtttcagct gcttcctcag tctcaggctc tgttatctcc tctggagctt
     3241 ctgttgctgc ctcagtctct tcttccttag tctcttccgg ctgctctttg ctttcttcca
     3301 catcttcctt agtttccgga acttctgcgg tttcttcagc agctgtggtc tctttttctg
     3361 catccgttgg atttacaaca ttttctgttg tttcttcgtt cactgcttct gaaggctttt
     3421 ctgccggagc agtttctgca ggtttggaaa taatgtttct ttcttcatcc ccaaacttat
     3481 cttcataact ctttatggta atacgtttgg tggtcttttc cttttctttt ccgttttctg
     3541 ttcttgttac agttgtagaa cagctgatgg tatcttcccc gttatttacg taaaggaaat
     3601 aaacctcttc ctctcctgtt accatgtacg tatcatctgc atcagccgga agctttacaa
     3661 atacccttaa ctctgcctcc atgctgtctc cctggatgtc cggatagact tcatatacct
     3721 ttccgtctcc aaagaacagt gcttcaaact tttctgtggc accgtcggta aaatccaggc
     3781 tgtcctgtga taccacattt tcgtcttcga tggcctgacg gatggcatcc accaggtcag
     3841 caccgccaat ggtaaagtcc actttgtttc ctgatgctgc aaagctgaca tgcagatctg
     3901 ttcccacatt ggtgaagatc atggcaaatg ccagcataaa tgccaggaaa cgcttctgta
     3961 aactaaccat ttttcccgtt cgtttcttgc gtctgcttcg tcctctcata ccctgtttct
     4021 cctctctctg aattttgtgc aggccccatt tcctgccaaa ctccagtact tcctgaatcc
     4081 cgtttcacag tcattcagtt tatactttca tttatcttta tcatagcaga gatagcgtta
     4141 taccaggcgt tatatggatt tatattttca aaaaattttc aatttttcaa ataatttaca
     4201 gactctatga gtttttttct cagtcgttct tgtgtttttt tataacatat gctataattc
     4261 gcttcaatga gtttttttct cacatcttcg cattttggag gtttgtatgc tgaatcagtt
     4321 tgttttaaga aattacagat tatttaaaaa tgaaacattg ctggactttt ttccggctcc
     4381 tattaatgaa cacaaagctt ctctgctgac agcgcaggga gacggcgaat catttcttcc
     4441 tgttatctcc ctttacggtc caaacggctg cggaaaatct tctatattag aggcgctctg
     4501 gaatgtatgt cgtctggctg caggtgattt ttctcttatc acctcttccg tccgttccta
     4561 ctgccgtctg gataatacat gcagagagct tcctttaagt tttgacctgc tttttcgcag
     4621 aaatggtttt ctcttccgtt atcagcttga tgtaaaacaa ggcgctgtcc tggaagaaaa
     4681 tatgttttac ggcaaacccg gttctgatga tgccggcgtt ctttttgccc gcaaagcaaa
     4741 tgaactccat attggaaatg aagccgggaa aatggatttt tccactctcc cggccggggt
     4801 ctctctttta cgctatcttg acccaaaatc ttcctctgaa tgtgcaaaag ctgctgcatc
     4861 ctggttcagc caggttctgt ttttccggga acatgactat aaaaaagccc cggatctgcc
     4921 ctctgaagta gaagaacgtc aggtcatctg ccgtctgctc caggctatgg atattgatat
     4981 tttagattac tccattacaa aagaacaggg cttcgatgat ccatccctga tcctgaccca
     5041 tgggaaagca gatggaaata ccttttttgt ttctttcaat gaagaatcca caggcatcag
     5101 aaagctgctt accctgatcc cgttagtcgt aaaaagcctg cgtctgggaa gtcttctatt
     5161 agccgatgac ttagataacg tgctccatcc tcatctgcta cgctttatcc tgtctctgta
     5221 ccaggatcat gagaaaaatc ctcatcatgc gcagttagta tttaccagcc ataacacagc
     5281 gatccttaat ccggctgtta tgcgaagaga cgagatctgt ctgtgctgcc gcccggaagg
     5341 aacggatgct gttctctatc cactttcttc ttataaaaaa gaaaatggcc tcatcccaag
     5401 aaatgatgaa gcctatggta aacaatatct ggaaggacgc tatggtgcca accccaggat
     5461 ctgatgacta aaattgatct tttttccatt ttcccattgc tttaaactac gttccacacg
     5521 ttcacacacg gccaggacat gttcctggcc tgctccattt aacaggacca gatactggac
     5581 cgggctgtag caggtaaaca gatctccctt tcgcagactc tctcctatgg cattttttaa
     5641 catctcagat gtcgtctgca ccatttcgcc ttccagcaat gctccttttc catctacaag
     5701 agtaatgcag aataatgtat attcaaagga aaaacgttct gccatgcggc tcaccagacg
     5761 gtaactgtca ataaatgaag gatatgtact gtaatacggt ccctgggttt cttccctctc
     5821 ttttaaggta ttttcaatgt cctttagcat atcggaagta tagctgatct gtccgctcat
     5881 caggcggaaa cgctgcagca tggtttcaga aggcggaagc ccctgttctt caaaatactg
     5941 gactaccacc tgtttataaa cttccatggc ttcctgatac cgttttaaag cgatcaggca
     6001 gtccagcttc atcatctggc attcctcata aggatacagc ttgctggctg ctgtgcttaa
     6061 gtttaaaagc ctgtcatatt cccggtctgc ttttaaaagc cggcttgcca ggcgcaggca
     6121 tttaaaatac agctcctggt attttactcc gatcacattg acccattttt ctcctgtcat
     6181 ctggaaaagg aattccccat aatacaagct acaggcgtgg cacagttcct ctttttttcc
     6241 tgttgcaaga gcactttgag ctgctttttc aaaatttcta gcatcaatgg aaacagtcac
     6301 atttccctca tcccaccggt aaataccgcc atctgtgcag atatactcat gctccggcag
     6361 catttctttt aataatctgc gcagcctgaa aactgctgcc cttaaactgc cggatggatt
     6421 agcatactct ccatttccgt aaaatacatc taacagctct tccctgccca ctcccttctt
     6481 tcctgcatac aaaaggatca ggaaaagctg caggatcttc cctgtaagag cagtccctac
     6541 aggaaaaaac tccccttcat attccactga tatggttccc atcatccgca cagtcagtat
     6601 tcccttttta tccatatcat tgcccatatc atttcctgcc tttctgtgat aaggcgccgt
     6661 ttcgtaaata actgtactga taattaatag ctgcgcagct ggtcttcttc ctctttggct
     6721 gtcttatcga tggaacggat ctctgcctca tatccgtaag tgtcatttac tttaatatac
     6781 accttatctc ctgttttacg gcccttaatt gcctttccta agggagaatc aatacttata
     6841 agtcctttca tggagtttcc ccggactgtg gtcactaatt tatagctttc tgtttcatca
     6901 tcttccggaa tgtataattc cacccggtcg cccagaccta cttcatctga ttttgactca
     6961 tccgaaacaa tacgtgctgt ttttatcatt ttttccagat aacggatacg gctctcgtta
     7021 cggttcttat cctgctttgc cgccttatat tcaaaatttt cgcttaagtc gccctgggct
     7081 cttgcctctt ttactgcttc caatgcttct ttgcgcacca ccagcttacg gtattctatt
     7141 tcttcctgca tttttttaat atcgtctttt gtcagattat catacatgtt tatacctcct
     7201 gatccgcttt cctcggagtc tttcataatt tctttatttt cccttcagga attttatttt
     7261 ttctttatcg tctgctcaaa ctttgtccac aatttacctg tatattatta cttgtaaggt
     7321 tgatgagacc tttt
//
LOCUS       DBMDUA010000142         7236 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4434_length_7236_cov_1.835179, whole genome shotgun sequence.
ACCESSION   DBMDUA010000142 DBMDUA010000000
VERSION     DBMDUA010000142.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7236)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7236
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4434_length_7236_cov_1.835179"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="ACZPCL_10105"
     CDS             complement(<1..319)
                     /locus_tag="ACZPCL_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HIR1162856.1"
                     /translation="MRKSKEHFRHEWKYLINRAEKQLTDLRLRECMQLDPHAGEGGYM
                     IRSLYFDDYWNSAMEEKDAGVLMRKKYRIRIYNCSDKSIKLERKKKFGSYIYKEAAPL
                     TRQE"
     gene            complement(306..986)
                     /locus_tag="ACZPCL_10110"
     CDS             complement(306..986)
                     /locus_tag="ACZPCL_10110"
                     /inference="COORDINATES: protein motif:HMM:NF020337.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HIR1162857.1"
                     /translation="MGFEKNTNRGSWEVGVSNYWGNILFQRCLKSDAFREKLDQVIEE
                     LRRYLSEERLEEMVEQYAGVVQPYVEKMPDQLNEPLTPEEYEQVKAGLPKEVEQNYEM
                     YRESLEKPMPFYIGRPVTENGKLKINWDASYDFDAEDITYSVEIARDYQFTKVIYKEE
                     QTLIPEILVDIPDPGQYFVRIRATNESGKTQDAFDYYVTNTGKQYGMICFYINEDHTV
                     GVDAYEEE"
     gene            complement(944..2104)
                     /locus_tag="ACZPCL_10115"
     CDS             complement(944..2104)
                     /locus_tag="ACZPCL_10115"
                     /inference="COORDINATES: protein motif:HMM:NF020337.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HIR1162858.1"
                     /translation="MKKRISLLLSVWLAVSSALCGCNMVLQKNEKIQESSTDLATASE
                     ETEIQGVKKAEEQDISQVHLRDKDLLYSKEDETNVTTMYLTVSRGNDAENTNHSWEEI
                     NSYSVFDYEEMGVPRYQVAALLQVGDENGPVAGEVGYGETVPNATVQIRGQSSSKNPQ
                     KNYKIKLKENKGSWEGQQTIALNKHMSEGLRFRNKLCYDLLKGIPQIMSLRTRFVHLY
                     VKDTTSGKDAFEDYGLYTQVEQLNKTALEAHGLQKNGQLYKVNYFEFYRYEDTIMTED
                     TPGFDLSKFEERLEIKGDHDHTKLIQMLDDLNNYSIPVSDVLKQHFDEENLLYWMAFQ
                     ILVGNSDTQSRNCYLYSPTNSPIWYILPWDNDGSFFNTEWALKKIQIVEAGR"
     gene            complement(2088..3560)
                     /gene="pelG"
                     /locus_tag="ACZPCL_10120"
     CDS             complement(2088..3560)
                     /gene="pelG"
                     /locus_tag="ACZPCL_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide Pel transporter PelG"
                     /protein_id="HIR1162859.1"
                     /translation="MAGIGFELKKLFRRKGIFASFRAYGYAGVICTGPMLLGVLLLLG
                     VMFLCNLYNVSEHSRELIICMITYALLGSLTVTSFFSMAVTRFLADMLYEDRKTMILP
                     SFWGSTGILLAAGGILYGLFLLFAGIGPVLQLLCFGLFGELVVCWMAMNYLTAIKDYK
                     GIMLSFVAAVLVTFLLGFLFLCLGFPAEEGMMAAVFFGYCVMLIWDVVLLYRYFPQSE
                     EKPFAFLAWVDEFLPLAFTGLFVNLGLFSHLVIMWMGPLQVHVQGLFYGAPYHDVSAL
                     MAFLSILITTINFVVSMEVNFYPKYRNYYSLFNDEGSIRDLKQAEHEMLEVLDNELRF
                     TALKQIFATAVAISIGGIVLSYLPLGFNDLMEGYFRTLCVGYGIYAVANTMLLILLYF
                     TDYQGGLVASALFALVSSAATVISLFFSKVYFGFGFILGCAVFFLAVYIRLEQFTRRL
                     PYYILSRQPLVEEDKLGVFTKLGYFLDGERKKEKLHEKTN"
     gene            complement(3541..4962)
                     /gene="pelF"
                     /locus_tag="ACZPCL_10125"
     CDS             complement(3541..4962)
                     /gene="pelF"
                     /locus_tag="ACZPCL_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HIR1162860.1"
                     /translation="MKICLILEGCYPYVHGGVSTWMHQYITIMKEHEFVLWVIGAKAE
                     QKGKFVYDLPDNVTEIHEVFLDDALKIRENGVRYVRFSPEEECALGEVMDCKSPDWEI
                     LFRLYQERKINPMSYLKSEAFLRILETICEERYPYIAFADAFHTIRSMMLPVLYLLGT
                     FVPQADAYHAISTGYGGLLASLGSWKYKKPLMLTEHGIYTREREEEIIRASWVAPAFK
                     KQWIRFFYMLSDVIYKRACRVTCLFTNALKIQEDIGCAPEKCRVIENGVSYERFCEIP
                     LKEEDGWVDIGAVVRLAPIKDIKTMIYAFYELSSRMEHVRLHILGGVDDEEYAKECYD
                     LVKQMELQNIIFTGRVDIQKYMEKLDFTILTSISEGQPLSVLESMAARRPCVCTDVGC
                     CRELLEGKQGDPFGLAGYCVPPMYREGLADAMEKMCLSRNRRLRMGESGRKRVEKYFR
                     HEYMMEKYRKLYREVEEIGRNRI"
     gene            complement(4967..6214)
                     /locus_tag="ACZPCL_10130"
     CDS             complement(4967..6214)
                     /locus_tag="ACZPCL_10130"
                     /inference="COORDINATES: protein motif:HMM:NF021477.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2194 domain-containing protein"
                     /protein_id="HIR1162861.1"
                     /translation="MIWNITLSERAKLYAWSSGKYKIPLVWENDYGSGKFVVVNLGIY
                     VKAVRGIFSAAYSLLSDCTIYPVINGSAFYLDDFPSPVPGGNGEYIYRDYGINVRDFY
                     ANIWWPDILALAEKYGVRYTGVVIENYGDQTDGVIEHQMETSRFQYFGNMLLRHGGEI
                     GYHGYNHQPLALGNVKYGDILPYNTWADTEAMENAMTELLQFCREMYPEASMSVYVPP
                     SNVLSEEGRQLLAAKCPEIRTIASNYFTGEFAYEQEFEVAEDGIVEQPRIISSAVIDD
                     YMQLCAFSELNMHFVNTHFMHSDDLLDEDRGAALGWETLKENLDRYMGWLYTSAPLLR
                     NLTGSELSGAIQRYSAAAAEKERTADGFCFHIENRYDTAYFFVRFSKEKPLAVTGGTM
                     EQLTDSLYLLCADSDEVQVKTDR"
     gene            complement(6221..6766)
                     /locus_tag="ACZPCL_10135"
     CDS             complement(6221..6766)
                     /locus_tag="ACZPCL_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162862.1"
                     /translation="MIEKENLEKNTIRKKYGWFPKISILEDLKDQYQDFLDRRQKREN
                     WYRLILSRITGHAAVIKWGEILLFFGFSEILHRVLGNSVQFRYIDVRLLYVVIIGSIY
                     GLNEGVAAAFLAVCALTVSYIREGINGLTLFYEPSNWLPYILYFVVGAVCGYSQMKNR
                     KDIAFANEENALLKDKFLFIL"
     gene            complement(6869..>7236)
                     /locus_tag="ACZPCL_10140"
     CDS             complement(6869..>7236)
                     /locus_tag="ACZPCL_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162863.1"
                     /translation="LEYCRGRAIQFLYITNENGKGDNLLLRNMADDICRYYREKAGVS
                     LKVVSAPCLYSARYSKDWLYKTFQSLEKGKMPEFPKQNEDMADFLYLDDLGELIFRIF
                     DNWDQDDRSLEIPDVFHIR"
BASE COUNT         2050 a         1854 c         1198 g         2134 t
ORIGIN      
        1 cttcctgccg tgtcagcggc gcagcttctt tgtatatgta actgccgaat tttttctttc
       61 tctcaagttt aatgcttttg tcactgcaat tatagatcct gatcctgtac tttttacgca
      121 tcagtacacc tgcatccttt tcttccatgg cactgttcca gtaatcatca aaatacaggc
      181 tgcgaatcat gtaaccgcct tcccctgcgt gaggatccag ctgcatgcat tcccttaagc
      241 gcagatctgt cagttgcttc tcagccctat tgatcaggta tttccattcg tgacgaaagt
      301 gttccttact cttcctcata tgcgtctact ccaaccgtat gatcttcgtt aatataaaaa
      361 cagatcatgc catattgttt tccggtattt gttacataat aatcaaaggc atcctgtgtc
      421 tttcccgatt catttgtggc ccgtatacgc acaaaatatt gcccagggtc cggaatatct
      481 accaatatct ccgggatcaa cgtctgttct tccttgtaga tcaccttggt aaactgataa
      541 tcccgggcaa tttccacact atatgtaata tcttccgcat caaaatcgta ggaagcatcc
      601 caatttattt tcagttttcc attttctgtc acaggtcttc caatataaaa cggcattggt
      661 ttttcaaggc tttccctgta catctcataa ttttgttcta cttctttcgg aagccctgct
      721 tttacctgtt catactcctc cggtgtaagt ggttcattca actgatccgg cattttctct
      781 acataaggct gtacaacacc tgcatactgt tccaccattt cttccagccg ttcttcactg
      841 agatatctgc ggagttcttc tatcacctga tccagttttt cacggaaagc atctgatttc
      901 aaacatcttt gaaataaaat atttccccag taattgctga ctcctacctc ccagcttcca
      961 cgatttgtat ttttttcaaa gcccattctg tattaaaaaa gcttccatca ttatcccatg
     1021 gcaaaatata ccatattggc gaatttgtcg gactgtacaa ataacaattc cggctctgcg
     1081 tatcactgtt tccaactaaa atctgaaatg ccatccagta cagcagattt tcctcatcga
     1141 aatgctgctt taacacatcg ctaacaggta ttgaataatt attcagatca tccagcatct
     1201 ggattaattt cgtgtgatcg tgatcccctt tgatctccag cctttcttca aattttgaca
     1261 gatcaaaacc tggtgtatct tctgtcatga tcgtatcttc atagcgataa aactcaaaat
     1321 aatttacttt atagagctgc ccgtttttct gaagtccatg tgcctccaaa gctgttttat
     1381 ttaactgttc tacctgtgta tacaaaccat aatcttcaaa tgcatctttc ccggaagttg
     1441 tatcttttac ataaagatgg acaaagcggg ttctgaggct cattatctgt gggataccct
     1501 taagaagatc ataacataat ttgtttctga agcgtagtcc ctctgacata tgcttgttta
     1561 gcgcgatcgt ctgctggcct tcccatgatc ccttattctc tttcaacttg atcttataat
     1621 ttttctgggg atttttactg gaactttgtc ccctgatctg cacagttgca ttcggtactg
     1681 tctcaccgta tccaacctct cctgcaacag ggccgttttc gtctcccacc tgtaaaagag
     1741 ctgccacctg ataccttggc actcccattt cttcataatc aaatacagaa tatgaattaa
     1801 tttcttccca actgtgattt gtattttctg catcatttcc acgactgact gtcagataca
     1861 tagtagtcac atttgtttca tcttcctttg aatataaaag gtccttatcc cgcagatgta
     1921 cctggctaat atcctgttcc tctgcttttt ttacaccttg tatctccgtt tcttctgatg
     1981 cggtcgctaa atccgtactg ctttcctgaa ttttttcatt tttctgcaaa accatattgc
     2041 atccacaaag agcagatgaa accgcaagcc aaacgcttaa tagtaaacta attcgttttt
     2101 tcatgaagtt tttccttctt tctctcacca tcaagaaaat atccaagttt tgtaaacacc
     2161 cccagtttat cctcctccac taaaggctgt ctgctgagaa tataatatgg aagcctcctg
     2221 gtgaattgtt ctaatctgat ataaactgcc aggaaaaaaa ctgcacatcc cagaataaat
     2281 ccaaagccaa aatacacttt ggaaaagaac agggatatca ccgttgcagc tgaggaaacc
     2341 aaagcaaaca gagccgatgc cacaagtcca ccctgataat ctgtaaaata taacaggatc
     2401 aaaagcatgg tattcgcaac tgcatagatc ccatacccaa cgcagagtgt cctgaaatat
     2461 ccttccataa gatcattaaa tccaagagga agataactca ggacgattcc tcctatggaa
     2521 atagcaacag ctgttgcaaa tatctgcttc agtgccgtaa atctcagttc attatcaaga
     2581 acctccagca tctcatgctc tgcctgtttc aaatcacgga tggatccttc atcattgaac
     2641 agactgtaat aattcctgta tttggggtaa aaatttactt ccatggatac cacaaaattt
     2701 atagtggtga tcagaatact taaaaatgcc atcagggctg acacatcatg gtaaggcgca
     2761 ccataaaaca atccctgaac gtgtacctgt aatggcccca tccacataat gacaagatga
     2821 gaaaaaagcc ccagatttac aaacaggcct gtaaatgcca gagggagaaa ctcgtctacc
     2881 cacgctaaaa acgcgaatgg tttctcttca ctttgcggaa aataacggta caaaagcaca
     2941 acatcccaga taagcattac acagtatcca aaaaatacag ctgccatcat accttcctct
     3001 gccggaaatc ccaggcagag aaatagaaac cccagcagaa atgtcacaag aacggctgca
     3061 acaaatgaca gcatgatccc tttataatct ttgatcgctg tcaaatagtt cattgccatc
     3121 cagcagacca ccagttcacc aaacaatcca aaacagagaa gctgcagcac cggtccgatc
     3181 cctgcaaaca gcaaaaagag tccatacaag ataccgccag ctgccagcaa aatcccggtt
     3241 gatccccaaa aagaaggaag gatcattgtt tttctatcct cataaagcat gtccgccaga
     3301 aatctggtaa ctgccatgga aaaaaaactg gtaactgtaa gggaaccgag gagagcatag
     3361 gtgatcatac agatgatcag ctccctgcta tgttctgata cattgtatag attgcagaga
     3421 aacattacac ccagcagaag aagaacacct aacagcatcg ggcctgtaca gatcacacct
     3481 gcatatccat atgcacggaa agatgcgaaa atcccttttc tgcgaaacag tttttttaac
     3541 tcaaatccga ttcctgccaa tctcttctac ctctctatac agctttctat acttttccat
     3601 catatattca tgccggaaat acttctccac acgttttctt ccgctttctc ccatcctcag
     3661 tctcctgttt cttgaaagac acattttttc catcgcatca gccagccctt cccgatacat
     3721 cggcggcaca caatatcctg caagtccaaa aggatcaccc tgcttacctt ccaacagttc
     3781 cctgcaacag ccaacatctg tacagacaca gggacgtctt gctgccattg attccagtac
     3841 agacaacggc tggccttccg atatacttgt caggatcgta aaatccagct tttccatata
     3901 tttttgaata tctacccttc cggtaaaaat gatattttga agctccatct gtttaaccag
     3961 atcataacat tcttttgcat attcttcatc atccacacct cccaggatat gaagccggac
     4021 atgttccatt cttgaggaaa gctcataaaa tgcatagatc atcgtcttga tgtctttgat
     4081 cggggccaga cggaccactg cgccaatatc cacccagccg tcttcttcct ttaatgggat
     4141 ttcacaaaaa cgctcataac tgaccccgtt ctcaatcacc cggcactttt caggggcaca
     4201 gccgatgtct tcctgtatct tcaaggcatt tgtaaacagg catgtcaccc ggcatgccct
     4261 cttatagatc acatcagaga gcatgtagaa aaaacggatc cactgctttt taaatgccgg
     4321 agcaacccag gatgcacgga tgatctcctc ctcccgttcc cttgtgtaaa tgccatgttc
     4381 cgtcagcatc agtggttttt tatatttcca gcttccaagg gatgccagaa gtcctccgta
     4441 accagtagaa atggcgtgat atgcatctgc ctggggtaca aatgttccaa gaagatagag
     4501 caccgggagc atcatcgaac ggatcgtatg aaatgcatcc gcaaaggcaa tatacggata
     4561 gcgctcttca cagatcgttt ccagaattct taaaaatgcc tcacttttca gataggacat
     4621 gggattgatc tttctctcct ggtacagacg gaacaaaatc tcccagtcag ggcttttgca
     4681 gtccatcact tctcccagcg cacattcctc ttccggcgaa aaacgtacat accgtacacc
     4741 gttttcccgg attttcaatg catcatccag aaatacttcg tgaatttccg tcacattatc
     4801 cggaagatca taaacaaatt ttcccttctg ttctgctttt gcgccaatga cccacagcac
     4861 aaattcatgt tctttcataa ttgtaatata ctgatgcatc catgttgaaa caccgccatg
     4921 aacataagga taacatcctt ccaaaatcaa acatattttc attgcatcac ctgtccgtct
     4981 tcacctgcac ttcatcgctg tctgcgcaaa gaagatacag gctgtctgtc agctgttcca
     5041 ttgtcccacc tgtcactgcc agcggctttt cttttgaaaa ccgcacaaag aaatacgctg
     5101 tatcatatcg gttttcaata tgaaaacaaa atccgtctgc tgtacgttct ttctctgcgg
     5161 ctgccgcgct gtagcgttgg atcgcacctg aaagttccga tcccgtcaga tttcggagca
     5221 aaggagcaga cgtataaagc cagcccatat atctgtccag attttccttt aacgtttccc
     5281 atccaagtgc tgctccacgg tcctcatcca gaagatcatc cgaatgcata aaatgagtat
     5341 ttacaaaatg catattcagt tcggaaaagg cacaaagctg catataatca tcgatcactg
     5401 cactggaaat tatccttggc tgctccacaa ttccatcttc tgccacttca aattcctgtt
     5461 cgtatgcgaa ttcacctgta aaatagttgc tggcaattgt tctaatctcc gggcattttg
     5521 ccgccaacag ctgccgcccc tcttctgaaa gaacattgga tggcggaaca tacacactca
     5581 tggaagcctc cggatacatt tcccggcaga actgcaaaag ttctgtcatg gcattttcca
     5641 ttgcctccgt atcagcccat gtattatacg gaagaatgtc accatacttt acatttccaa
     5701 gggccagtgg ctgatgatta tacccatgat atcctatttc tcctccgtga cgcagaagca
     5761 tatttccaaa atactggaac ctggatgttt ccatctgatg ttcgattacc ccgtctgttt
     5821 gatccccata gttttcaatc acaactcctg tatatctaac accatatttt tccgccaggg
     5881 ccaggatatc cggccaccag atgtttgcat aaaaatctct gacatttata ccataatcac
     5941 ggtatatata ctctccgttt ccacccggta ccggggaagg aaaatcatcc agataaaaag
     6001 cagatccatt gataaccggg tagatggtac aatctgacag aagactgtat gcagctgaaa
     6061 aaatacctct gactgccttt acatatattc ccagatttac taccacaaat ttcccagaac
     6121 cataatcatt ttcccagacc agagggattt tatatttccc gctgctccag gcatacagct
     6181 ttgccctttc acttaatgtt atattccata taatgcactg ttaaaggata aacaggaatt
     6241 tatcctttaa cagtgcattt tcttcatttg caaacgcaat atcttttctg tttttcatct
     6301 ggctataacc acatacagcc ccaaccacga aatataaaat atacggcagc cagttagatg
     6361 gttcataaaa cagtgtcaaa ccgtttatgc cttctctgat gtaagaaact gtcagagcac
     6421 agactgccag aaaagctgcc gctacccctt cattcaatcc ataaatagat ccaatgatca
     6481 ccacatacaa aagacgtaca tcaatataac gaaactgtac agaatttcca agaactctgt
     6541 ggaggatttc agaaaatccg aaaaagagaa ggatttctcc ccatttaatg accgcggcat
     6601 gacctgtaat cctgcttaaa ataagtctgt accagttttc acgtttctgc cttctatcaa
     6661 gaaaatcctg atactgatcc tttaaatctt ccagaatact gatcttcgga aaccagccat
     6721 attttttccg gatcgtattc ttttccagat tctctttttc aatcatattc ctgctgtccg
     6781 gtacagtctt cacagttttt tcccgttttt ctgaacggtc tgcttttagc gcaggaaaca
     6841 attttaagat tgcagtttcc agttctccct aacgaatatg gaacacatcc gggatttcaa
     6901 gactcctgtc atcctgatcc cagttatcaa aaatacgaaa aatcagttct cccaggtcat
     6961 ccagatacaa aaaatcagcc atatcctcat tttgttttgg aaattcaggc atctttccct
     7021 tttccagtga ctgaaaggtc ttgtacagcc agtctttact gtatcttgca gaataaaggc
     7081 agggagcact cacaaccttt aacgaaacgc ctgctttctc ccgataatac ctgcatatat
     7141 catccgccat atttctcaga agcagattgt ctcctttccc gttttcattt gtaatatata
     7201 agaactggat cgcgcgtcct ctgcaatact ccaaaa
//
LOCUS       DBMDUA010000143         7192 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4457_length_7192_cov_2.613172, whole genome shotgun sequence.
ACCESSION   DBMDUA010000143 DBMDUA010000000
VERSION     DBMDUA010000143.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7192)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7192
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4457_length_7192_cov_2.613172"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            36..419
                     /gene="rbfA"
                     /locus_tag="ACZPCL_10145"
     CDS             36..419
                     /gene="rbfA"
                     /locus_tag="ACZPCL_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR1162864.1"
                     /translation="MRKNSIKNTRINMEVQRELSQIIRSEIKDPRIHPLTSVVAVEVT
                     PDLKYCKAYISVLGDEEAGKATIEGLRSAASFVRRELAHRVNLRNTPEIKFILDQSIE
                     YGVNMSKLIDEVTKDLHDDEEEGLE"
     gene            416..1384
                     /locus_tag="ACZPCL_10150"
     CDS             416..1384
                     /locus_tag="ACZPCL_10150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1162865.1"
                     /translation="MTVLEQALEGTKSVAILGHVRPDGDCLGSTLGLYNYLLASYPDI
                     QAAVYLEEASSKFAYLKGYDEIHHETDDKEYELCICLDSSDEERLGDFNVYLKRAKKS
                     LCIDHHVTNTRYCQVNLVAADASSASEVLFEQLNPDNVDKNVAECLYTGIVHDTGVFK
                     YSCTSAKTMEIAGFLMGKGVDFGSIIDNSFYKKTYVQNQIMGRALLESITFLDGKAIF
                     SALRQSDLDFYGVTGKDLDGIIDQLRLTEGVEVAIFLYETGPQQYKVSLRSQKIVDVS
                     QVAFYFGGGGHVRAAGCTMSGSIHDVINNLSLHIAKQLDAAAAGEV"
     gene            1385..2323
                     /gene="truB"
                     /locus_tag="ACZPCL_10155"
     CDS             1385..2323
                     /gene="truB"
                     /locus_tag="ACZPCL_10155"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR1162866.1"
                     /translation="MYHGIINVYKEPGYTSHDVVARLRGILRQKKIGHTGTLDPAAKG
                     VLPVCLGNGTRLCDMLTDRSKTYEAVLLLGQTTDTQDTTGTVLTEDHEKALELDEEQV
                     RQVILDFKGDYDQIPPMYSALKVDGKKLCDLARAGKEVERKARRVQILDISVEKIELP
                     RVWMTVSCSKGTYIRTLCEDIGAKLGVGGCMESLLRTRVDRFQVKDSLKLDEIEVLRD
                     EGKVGEYICSLEEALEGYLPVYTTAEGDKALKNGNPCFKQQLDLEKSTSLEERKSGDR
                     YRMYNSQGIFMGVYEYQEEKHWWKPWKMFMPDGEGN"
     gene            2323..3276
                     /locus_tag="ACZPCL_10160"
     CDS             2323..3276
                     /locus_tag="ACZPCL_10160"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR1162867.1"
                     /translation="MKYIADTVDFQLEEPTVVTLGKFDGRHRGHQKLLHTMEELKETL
                     GYATAIFTFSTAPLSLVTGEAATVITTSEERRHNMEKMGIDYLVEYPFTDDVRKMDPS
                     VFVKDILVKRMRAKAMVVGPDCSFGYKGAGNVELLRSLSKELGFELYVIQKEKDHRRD
                     ISSTYIREELSLGNVAKANELLGEPYAIHGIVVHGNHIGGPRLGFPTANIIPPANKRL
                     PKFGVYVSRVLAQGKYYKGVTNIGRKPTIHGDNPVGVETFIFDFDGDIYGKMIEVQLL
                     EFDRGEQKFASLEELKERIEKDKEFALKYFAENPLYNPVKI"
     gene            3382..3648
                     /gene="rpsO"
                     /locus_tag="ACZPCL_10165"
     CDS             3382..3648
                     /gene="rpsO"
                     /locus_tag="ACZPCL_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR1162868.1"
                     /translation="MISKEKKAEIIAQYGRKAGDTGSPEVQIAILTERIAELTAHLQQ
                     NPKDHHSRRGLLMMVGHRRGLLDYLKKTDLEGYRALIEKLGIRK"
     gene            3920..6037
                     /locus_tag="ACZPCL_10170"
     CDS             3920..6037
                     /locus_tag="ACZPCL_10170"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR1162869.1"
                     /translation="MFKSFSMDLAGRKLTVEVGRVAAQANGAAFMHYGDTVVLSTATA
                     SAKPREGIDFFPLSVEYEEKFYSVGKIPGGFNKREGKASENAILTSRVIDRPMRPLFP
                     KDYRNDVTLDNVVMSVDPDCSPELTAMLGSAIATSISDIPFDGPCATTQVGLIDGEFV
                     INPTSAQRQVSEMALTVASTREKVIMIEAGAKEVPEQKMIEAIFKAHEVNQEIIRFID
                     TIVAECGKEKHTYESCAVPEELFAAIKEIVPSEEMETAVFTDDKQTREANIAAITERL
                     EEAFAENEEWLPLLGDAIYQYEKKTVRKMILKDHKRPDGRAITQIRPLAAEIDILPRV
                     HGSGMFTRGQTQILNACTLAPLSEAQKIDGLDANETTKRYMHHYNFPSFSVGETKPSR
                     GPGRREIGHGALAERALVPVLPSEEEFPYAIRTVSETMESNGSTSQASICASTLSLMA
                     AGVPIKRMVAGISCGLVTGETDDDYIVLTDIQGLEDFFGDMDFKVGGTEKGITAIQMD
                     IKIHGLTRPIIEEAIARTREARMYILDNVMKPVISEPRKHLSQYAPKIKQITIDPQKI
                     GDVVGKQGKVINKIIEETGVKIDISDDGIVNVCGTDEAMIDRAIAIITGIVTDIEAGM
                     VFTGKVVRIMNFGAFVQLAPNKDGMVHISKLSDKRVAKVEDVVNIGDEVTVKVAEVDK
                     MGRINLTMRPSDLAEKKEEKEEN"
     gene            6055..>7192
                     /gene="gltX"
                     /locus_tag="ACZPCL_10175"
     CDS             6055..>7192
                     /gene="gltX"
                     /locus_tag="ACZPCL_10175"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR1162870.1"
                     /translation="MKVRTRYAPSPTGRMHVGNLRTALYAYLIAKHEGGDFLLRIEDT
                     DQERYVEGAVDIIYRTLEETGLVHDEGPDKDGGCGPYVQSERQASGIYMEYAKQLVEK
                     GEAYYCFCTPERLESLRKTVNGEEIMTYDKHCLHLSKEEIEANLAAGKPFVIRQNNPT
                     EGTTTFHDEIYGDITVDNSELDDMVLIKSDGYPTYNFANVVDDHLMGITHVVRGNEYL
                     SSSPKYNRLYDAFGWEVPVYVHCPLITDESHHKLSKRCGHSSFEDLVEQGFVPEAIVN
                     YVALLGWSPEGNNEIFSLEEMVKEFDYHRMNKSPSVFDMTKLRWMNGEYIKAMDFDRF
                     YKLAEPYLKEVIKKDLDLKKIANMVKTRIEVFPEIRDHIDFFEEL"
BASE COUNT         2183 a         1444 c         1815 g         1750 t
ORIGIN      
        1 gaatatagtt tcgtttttga aagaaaggga tcataatgcg taaaaacagc attaaaaata
       61 caaggatcaa tatggaggtc cagagggaat taagtcagat catccgttct gagatcaaag
      121 acccgaggat ccatccgctg accagtgttg tagccgtaga ggtaactccg gacttaaaat
      181 actgcaaggc atatatcagt gtgttaggtg atgaagaagc cggcaaggca accatcgaag
      241 gactgcgcag cgcagcttcc ttcgtgcgcc gggaactggc tcacagagtc aatttacgta
      301 acactcctga aattaaattt atactggatc agtctattga atatggcgta aacatgtcaa
      361 aactgatcga tgaagtgaca aaagatcttc atgatgatga ggaggaggga ctggaatgac
      421 ggttcttgaa caggcgttag aaggaacaaa gtctgtagcg attttgggcc atgtaagacc
      481 ggatggcgat tgcctcggct ctactctggg tctgtataat tatcttcttg cttcctatcc
      541 ggatatacag gcagctgtat atctggagga agcttcctca aaatttgcct atttaaaggg
      601 ctatgatgag atccaccatg aaacagatga caaagaatat gagctttgca tctgccttga
      661 cagcagtgat gaagaacgtc tgggggattt taacgtgtat ctgaagcggg cgaaaaaaag
      721 cctttgcatc gatcatcacg taaccaacac ccgttactgc caggtcaatt tagtggcagc
      781 tgatgcaagt tctgccagcg aagtcctttt tgaacagtta aatccggata acgtggataa
      841 aaatgtggca gaatgcctgt atacagggat cgtacatgat accggtgtgt tcaagtattc
      901 ctgcacttct gcaaagacga tggagattgc cggtttcctg atgggaaaag gcgttgactt
      961 tggaagcatc attgacaaca gcttctacaa aaagacttat gtgcagaacc agatcatggg
     1021 aagagcgctt cttgaaagca ttaccttcct tgatggaaag gccattttca gtgccctgcg
     1081 ccagtctgat ctggactttt acggtgtgac cggaaaagac cttgacggta tcatcgacca
     1141 gctgcgtctt acagagggag tggaggttgc tattttcctg tatgagacag gacctcagca
     1201 gtataaggta agtctgcgtt cccagaaaat cgtagatgta agccaggtag cgttctattt
     1261 tggcggcggc ggtcatgtgc gtgcagcagg ctgtaccatg tccggaagta ttcatgatgt
     1321 gatcaataat ctgtcccttc acattgccaa gcagttagat gctgcggcag ccggggaggt
     1381 ttaaatgtat cacggaatca ttaatgtgta taaagagccg ggctatacat cccatgatgt
     1441 tgtagcccgg cttcgaggga ttttaagaca gaaaaagatc ggccatacgg gaacccttga
     1501 tccggcggcc aagggcgtcc ttccggtgtg tctgggaaat gggacaaggc tttgtgacat
     1561 gcttacagac cggtccaaaa cctatgaggc agtgcttctt ttaggacaga ctacggacac
     1621 ccaggatacc acaggaacgg tgcttacgga agatcatgaa aaggcgttag aactggatga
     1681 agaacaggtg cgccaggtga tcctggattt taagggagat tatgaccaga tccctcctat
     1741 gtactctgct ttaaaagtag atggaaagaa gctttgcgac cttgcaagag caggaaaaga
     1801 agtagagcgc aaggcaagaa gagtacagat cttagatatt tctgttgaaa aaatagaact
     1861 gccaagagtc tggatgacag tcagctgttc caaaggaacc tacataagaa ctctctgtga
     1921 agatattgga gctaaattag gagtcggggg ctgcatggag tctctcctgc gtacccgtgt
     1981 agaccgtttt caggtaaaag acagtttgaa actggatgag atcgaagttt taagagatga
     2041 aggaaaagta ggagaatata tctgttctct ggaagaagcc ttagaaggtt atctgcctgt
     2101 atacacaaca gcagaagggg acaaggcttt gaaaaatgga aatccctgct ttaaacagca
     2161 gttagacctg gaaaaaagta catctttaga agaaagaaaa agcggagaca gataccgtat
     2221 gtacaacagc caggggatct ttatgggtgt atatgaatac caggaagaaa aacactggtg
     2281 gaaaccatgg aaaatgttca tgccggatgg agaaggaaac tgatgaaata tatagcagat
     2341 acagtggatt ttcagctgga agagcccact gtggtaactc ttggaaaatt tgacggaaga
     2401 cacagaggac atcagaagct tctgcataca atggaagaac tgaaagaaac actgggctat
     2461 gcaactgcta tttttacctt cagcacagcc ccattaagcc ttgttacagg tgaagctgcc
     2521 actgtgatca caaccagtga agaacgacgt cataatatgg aaaaaatggg gatcgattac
     2581 ctggtggagt atccttttac agatgatgtg cgtaaaatgg acccttctgt atttgtaaag
     2641 gatatcttgg taaaacggat gcgggcaaaa gccatggtag tggggccgga ctgcagtttt
     2701 ggctacaaag gcgccggaaa tgtagaactt ctcagatctt taagcaaaga gctgggattt
     2761 gaactttatg tgatccaaaa agaaaaagat cacagaagag acatcagcag cacctatatc
     2821 cgggaagaac tttccctggg aaatgtggca aaagcaaatg aattgttagg agaaccatat
     2881 gccatccacg gcattgtagt ccacggcaac catatcggag gaccgagact gggctttcca
     2941 acagccaata tcatcccgcc ggcaaacaaa cgtctgccca aattcggggt ctatgtatcc
     3001 agagtccttg cacagggaaa atattataag ggagttacta atatcggcag gaaacctacg
     3061 atccatgggg ataatccggt aggcgtagag acatttatct ttgattttga cggagacatt
     3121 tacggaaaaa tgatcgaggt ccagcttttg gagtttgaca ggggagaaca gaaatttgct
     3181 tctttagaag agttaaaaga acggatcgaa aaagacaaag aatttgctct taagtatttt
     3241 gcggaaaatc ccctttacaa cccggttaaa atatgataca gtaaccaagt ataaaagaac
     3301 gccgaagccc cgttgcctgg tgactccagc cggcagcctg gtttaaggtg agaaaattaa
     3361 attattttag gaggaattac catgatttct aaggaaaaga aagcagagat tatcgcacag
     3421 tacggacgta aggcaggaga taccggttca ccagaagttc agatcgctat tctgacagag
     3481 agaattgctg aactgaccgc tcatcttcag cagaatccaa aggatcatca ctccagaaga
     3541 ggacttctga tgatggttgg acacagaaga ggacttcttg attacctgaa gaagaccgat
     3601 ctggaaggct accgtgctct gatcgaaaag ctgggaatca gaaagtaatt atcatgaaag
     3661 tgttgccggg cggctatgtg gattgcgtgc ttgtacgcaa ggccgcatag acatttggca
     3721 gcgggctttc attcatgtta gggtggagtt tactccaccc tatatttgca tgaagacagg
     3781 tgcaaaagga acactccggg accgctgatg tgtcccaaac agatcgtttg ggatagatca
     3841 gtagtttcgg cgtcttttgc cctgtaaaaa acttgtatat ttacagaacg ttacattaaa
     3901 agagaaaagg agacacccca tgtttaagag tttcagtatg gacctggctg gccgtaagct
     3961 gactgtagaa gttggccgcg tagcagctca ggcaaacggt gcagcattta tgcattacgg
     4021 cgatactgta gtactttcta cagcaaccgc atctgcaaag ccaagagaag gcattgattt
     4081 cttcccttta agtgtagaat atgaggagaa gttctactct gtaggaaaga tcccaggcgg
     4141 cttcaataag agagaaggaa aagcatctga aaatgccatc cttacctccc gtgttatcga
     4201 ccgccctatg cgtcctttat tcccaaagga ttaccgcaat gacgtaacct tagacaacgt
     4261 agttatgtct gtagatccag actgcagccc agagctgact gctatgcttg gttctgctat
     4321 cgctaccagc atttctgata tcccatttga cggaccttgc gctactaccc aggttggtct
     4381 tattgatggt gaatttgtta tcaacccaac ttccgctcag agacaggtat ctgagatggc
     4441 tctgactgta gcttctacca gagagaaagt tatcatgatc gaagctggtg ccaaggaagt
     4501 tcctgagcag aagatgatcg aggctatttt caaagctcac gaagtaaacc aggagatcat
     4561 ccgctttatc gataccatcg tagctgagtg cggaaaagaa aagcacacct acgaaagctg
     4621 cgcagttccg gaagaattat ttgcagcgat caaggagatc gttccttctg aagagatgga
     4681 aacagctgta tttaccgatg acaagcagac ccgagaggca aatattgcag ctattactga
     4741 gcgtctggaa gaggcatttg ctgagaacga agagtggctg ccattattag gcgatgctat
     4801 ttatcagtat gagaagaaga ctgtccgcaa gatgatctta aaagaccaca agcgtccaga
     4861 cggccgtgcg attacacaga tccgtccttt agcagctgag atcgacatcc ttccaagagt
     4921 acatggttca ggtatgttca cccgtggaca gacccagatc ttaaatgcct gcacactggc
     4981 accattatct gaggctcaga agatcgatgg cttagatgca aatgagacaa ccaagcgtta
     5041 catgcatcat tacaacttcc cgtccttctc cgtaggcgag acaaagccaa gcagaggacc
     5101 gggccgtcgt gagatcggac atggcgcact ggctgagcgt gcattagttc ctgtacttcc
     5161 aagtgaagaa gagttcccat atgcgatccg tactgtatct gagactatgg agtctaatgg
     5221 ttctacttcc caggcaagta tctgtgcatc cactctgtcc ttaatggcag caggtgtacc
     5281 gatcaagcgt atggtagctg gtatctcctg tggtctggta acaggtgaga ctgatgacga
     5341 ttacatcgtt ctgaccgata tccagggtct ggaagacttc ttcggcgata tggactttaa
     5401 ggtaggcggt actgaaaagg gtatcactgc tatccagatg gatattaaga tccatggtct
     5461 gacccgtccg atcattgaag aggcaattgc aagaacccgt gaagcaagaa tgtacatctt
     5521 agacaatgtt atgaagccag ttatctccga gcctagaaag cacttaagcc agtatgctcc
     5581 aaagatcaag cagatcacca ttgatcctca gaagatcggc gatgtagttg gtaagcaggg
     5641 caaggtgatc aacaagatca tcgaagagac cggcgttaag atcgacatca gcgatgatgg
     5701 tattgtaaat gtttgcggta ctgacgaggc tatgatcgac cgcgctatcg ctattattac
     5761 aggaattgtt actgatatcg aagctggaat ggtatttacc ggcaaggtag tacgtatcat
     5821 gaacttcggc gcatttgtac agctggcacc aaacaaagac ggtatggtac acatctccaa
     5881 gctgtctgat aagcgtgttg caaaggtaga agatgttgta aacatcggcg acgaagtaac
     5941 tgttaaggtt gcagaagttg acaagatggg aagaattaac ctgaccatga gaccaagtga
     6001 cctggcagag aagaaggaag aaaaagaaga aaactaaaat cagagggtga gaaaatgaaa
     6061 gtaagaacaa gatatgcacc aagccctaca ggaagaatgc atgttggaaa tttgagaaca
     6121 gctctgtatg cgtacctgat cgcaaaacat gagggaggcg atttcctttt aagaattgaa
     6181 gatacagacc aggaacgtta tgtagaaggc gctgtagata tcatttacag aaccctggaa
     6241 gaaaccggtc tggtccacga tgagggacca gacaaggatg gcggctgcgg cccttatgtt
     6301 cagagtgagc gccaggcttc cggaatttat atggagtatg caaagcagct ggtagaaaaa
     6361 ggagaagcat attactgctt ctgtacacca gagcgtctgg agtccttaag aaagactgta
     6421 aacggtgagg agatcatgac ctatgataag cactgtctgc acctttccaa agaggaaatc
     6481 gaggcaaatt tggctgctgg aaagccattt gttatccgtc agaataaccc gactgaggga
     6541 actaccacct tccatgacga gatctacgga gatatcactg tagataactc tgaactggat
     6601 gatatggtac tgatcaaatc tgatggttat cctacctaca actttgcaaa cgtagtagac
     6661 gatcatctta tgggcattac ccatgtagta agaggaaatg agtatctttc ttcttcacca
     6721 aagtacaacc gtctgtatga cgctttcggc tgggaagtgc cggtttatgt acactgcccg
     6781 ctgatcacag atgaaagcca tcacaagtta agcaagcgct gcggacattc ctcttttgag
     6841 gacctggtag aacagggctt tgttccggaa gcaatcgtaa actatgtggc acttcttggc
     6901 tggtctccag aaggcaataa cgagatcttc tctctggaag agatggtaaa ggaatttgat
     6961 taccaccgta tgaataagtc tccgtctgtt tttgatatga ccaagcttcg ctggatgaac
     7021 ggcgagtata tcaaggcaat ggactttgat cgtttctata agctggctga gccatactta
     7081 aaagaagtga tcaagaaaga cttagatctt aagaagattg caaatatggt aaagaccaga
     7141 attgaagtat tcccggagat cagggatcac attgatttct ttgaggaact tc
//
LOCUS       DBMDUA010000144         7166 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4472_length_7166_cov_1.814296, whole genome shotgun sequence.
ACCESSION   DBMDUA010000144 DBMDUA010000000
VERSION     DBMDUA010000144.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4472_length_7166_cov_1.814296"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="ACZPCL_10180"
     CDS             complement(<1..208)
                     /locus_tag="ACZPCL_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA];
                     GO_process: GO:0090071 - negative regulation of ribosome
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsfS/YbeB/iojap family protein"
                     /protein_id="HIR1162871.1"
                     /translation="MNSKEMVKIAYEALSDKKGQDIKIIDIQSVSVLADYFIIADGSN
                     PNQVQAMADNVEEMLGKAGCECKQI"
     gene            complement(222..815)
                     /gene="yqeK"
                     /locus_tag="ACZPCL_10185"
     CDS             complement(222..815)
                     /gene="yqeK"
                     /locus_tag="ACZPCL_10185"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HIR1162872.1"
                     /translation="MSNHCSQIMMLRSKLKTKLDPMRYEHSLSVSFTCMNLAMRYGYD
                     IDKAELAGLMHDCGKRFADEIILKKCISHKIPVTDAEMKALPVLHAKYGAWLAENKYG
                     IEDPEIISAIACHTTGKADMSVLDKIVYIADYIEPRRYKADNLPQMRRLAYEDLDQTM
                     YEILKSTLEYLAKKGADADPMTATAYEYFKQLVAAEK"
     gene            complement(837..1457)
                     /gene="nadD"
                     /locus_tag="ACZPCL_10190"
     CDS             complement(837..1457)
                     /gene="nadD"
                     /locus_tag="ACZPCL_10190"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR1162873.1"
                     /translation="MAKIGILGGTFDPVHNGHLALGKQAYEQFKLDEIWFMPSGHPPH
                     KKSRLVTQGKEREDMVKLAIASVPYFVYSDFELKREGNTYTAQTLTLLREAYPQHEFY
                     FIIGADSLYEIEQWYHPELVIKQAVLLAAARPYEKEHPNFEKQVKYLQEKFDARIGVI
                     RFEEMDVSSRQIRKAVALGQSIKDLVPGPVAGYIRIHGLYREAFED"
     gene            complement(1483..1776)
                     /gene="yhbY"
                     /locus_tag="ACZPCL_10195"
     CDS             complement(1483..1776)
                     /gene="yhbY"
                     /locus_tag="ACZPCL_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HIR1162874.1"
                     /translation="MTSKQRSYLKGLAMNLDPIFQIGKSSLTPEVTQAVSEALEAREL
                     VKITVLKNCLDDGRSIAEVLGERTHSEVVQVIGRKIVLYRPAKDETKRKIVLP"
     gene            complement(1824..3113)
                     /gene="obgE"
                     /locus_tag="ACZPCL_10200"
     CDS             complement(1824..3113)
                     /gene="obgE"
                     /locus_tag="ACZPCL_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR1162875.1"
                     /translation="MFADRAKVFIKSGKGGDGHVSFRRELYVPNGGPDGGDGGRGGDV
                     IFQVDTGKNTLVDFRHIHKYVAKDGQEGGKRRCHGADADDLIVKVPEGTVIKDFESGK
                     VIADMSGDNKREVILKGGKGGLGNMHFATSTMQVPKYAQPGQPGQELWVTLELKVIAD
                     VGLVGFPNVGKSTLLSVVSNARPEIANYHFTTLNPHLGVVDLGDGAGFVMADIPGLIE
                     GASEGVGLGHSFLRHIERTKVLVHVVDGASVEGRDPLEDILTINKELEAYNPELLKRP
                     QVIAANKMDACFSEDDSNDIIARLKAEFEPKGIKVFPISAVSRQGVKELLWHVNDLLK
                     TVDSAPVVFQKEFEIEYQGDRNLPYTVTRADDGAYVVEGPRIEKMLGYTNLDSEKGFD
                     FFQKFLKNTGILEDLEKAGISEGDTVRMYGLEFDYYK"
     gene            complement(3231..3521)
                     /gene="rpmA"
                     /locus_tag="ACZPCL_10205"
     CDS             complement(3231..3521)
                     /gene="rpmA"
                     /locus_tag="ACZPCL_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR1162876.1"
                     /translation="MLKMNLQLFAHKKGVGSTKNGRDSESKRLGAKRADGQFVLAGNI
                     LYRQRGTHIHPGNNVGKGGDDTLFALVDGVVKFERKGRDKKQVSVYPRAINE"
     gene            complement(3525..3866)
                     /locus_tag="ACZPCL_10210"
     CDS             complement(3525..3866)
                     /locus_tag="ACZPCL_10210"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HIR1162877.1"
                     /translation="MIQTTVFKKSTDSGNDLYCGIVIKGHAGYAEEGEDIICAAVSAL
                     AINFYNSVETFTDDGFEGIAGQEDVQFDFRFTSDISPESQLLMNSLVLGLQNIENDYG
                     KSYINIRFKEV"
     gene            complement(3878..4186)
                     /gene="rplU"
                     /locus_tag="ACZPCL_10215"
     CDS             complement(3878..4186)
                     /gene="rplU"
                     /locus_tag="ACZPCL_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR1162878.1"
                     /translation="MYAIIATGGKQYKVAEGDVIKVERLGAGAGETVTFDQVLAVNNG
                     ELQIGCPTVAGATVTATVEKEGKGKKVIVYKYKRKTGYHKKNGHRQLFTQVKIEKINA
                     "
     gene            complement(4366..5805)
                     /locus_tag="ACZPCL_10220"
     CDS             complement(4366..5805)
                     /locus_tag="ACZPCL_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR1162879.1"
                     /translation="MNHSIIIYMLGWIMNIEAALMLIPILTAVVYKEGIISCYAGVAV
                     VCAVLGFLCTRKKPKVRMFFAREGFVLVSLGWLVLSFFGCLPFVLSGEIPHFIDALFE
                     IVSGFTTTGSSILPEVEKLSKASLIWRSFSHWIGGMGVLVFILAILPMAGDYDMHIMR
                     AESPGPSVGKLVPKIRITAKLLYIIYFCITVATAVFLLIGKMPVFDTICMSLGAAGTG
                     GFACRNSGQAGYTVYQQAVLSIAMILFGINFNVYYLLLIRKPKEAFKCEEMRGYLAII
                     LIAVVLITVNIRSMFPSLFLAAHHALFQVASIITTTGYSTVDFDKWPEFSKCIIVLLM
                     FIGACAGSTGGGMKVSRIMIAFKEAKREISTMIHPRSVKVLKYEGKVIGNDTLRVLNC
                     YIIVYFMLFVASVLIVSLDNYDFTTSFTAVAANFNNIGPGLSVVGPASNFSMMSYLSK
                     SVLIFDMLAGRLELFPVLVLLSPGTWKRG"
     gene            complement(5819..7165)
                     /gene="trkA"
                     /locus_tag="ACZPCL_10225"
     CDS             complement(5819..7165)
                     /gene="trkA"
                     /locus_tag="ACZPCL_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HIR1162880.1"
                     /translation="MIVGCGKVGTTLAEQLNRENHDITLIDTDDEALKNISDNVDLMS
                     VTGNGAIYQVQMEAGIQEADLMIATTNSDELNMLCCLIAKKAGNCHTIARIRNPEYSA
                     EIGYIREELNLSMAINPELAAAREIARLLRFPSAIKIEPFAKGRIELLKLLIPDHSNL
                     DQLKVMDVVNKLKSNVLICAVERGDDVVIPDGNFVMQKGDKISFIASHTDSADFFKKA
                     GIVNNSVKTAMFVGGGKLTYYLCNQLADTKIKIKIIEQDEARCRQLSELLPNAMIIHG
                     DGSDQQVLLEEGILQTEAFASLTGFDEENIMLSLYAASKSNAKLITKVNRIAFESVIN
                     SLNLGSLIQPKMLTADLILQYVRAMQNSMGSNVETLYKIVADKAEALEFRVKEGSPLL
                     GIPLERLQLKNHLLVACINRSGCIITPRGKDTIEAGDTVIIVTTHTGLNDLTDILK"
BASE COUNT         1875 a         1746 c         1373 g         2172 t
ORIGIN      
        1 cgatctgttt gcattcgcag cctgctttgc caagcatctc ttctacattg tcagccattg
       61 cctgtacctg gtttgggttg cttccgtctg caatgatgaa ataatcagca agaacagata
      121 cagactggat atcgatgatc ttgatatcct ggcccttttt atcacttaac gcctcgtagg
      181 cgatctttac catttcctta gaattcattc tatttctcct tttacttttc ggctgctacc
      241 agctgcttga agtactcata agctgtagcg gtcataggat cagcatctgc gcccttcttt
      301 gcaagatatt ccagtgtact ttttaatatt tcatacatgg tctgatccag gtcttcatag
      361 gcaagccttc tcatctgagg cagattatcc gctttatagc gtcttggttc aatataatct
      421 gcaatatata cgatcttatc cagaacggac atgtccgcct tacctgttgt atgacaggca
      481 atggcactta tgatctccgg atcttcgatc ccatatttat tttcagccag ccaggcaccg
      541 tacttggcgt gaagtaccgg aagtgccttc atttctgcat cagtcacagg aattttgtgg
      601 ctgatacatt tctttaagat aatctcatct gcaaaacgtt taccgcagtc atgcatcagt
      661 cctgcaagtt ctgccttatc tatatcatat ccataacgca tggcaagatt catacaggta
      721 aaagatacgc tgagtgaatg ttcataacgc ataggatcca gttttgtttt cagtttactg
      781 cgcagcatca tgatctggct gcaatgattg ctcatctgca taactgtctc actcctttaa
      841 tcttcaaagg cttctctgta aagtccatgt atcctgatat atccggcaac cggacctgga
      901 accagatctt taatagattg tcccagcgca actgcttttc gtatctgtcg ggaggataca
      961 tccatttctt caaaacgtat cactccgatc ctggcatcaa atttttcctg cagatatttt
     1021 acctgctttt caaaatttgg atgttctttt tcatagggtc ttgctgctgc cagtaaaact
     1081 gcctgtttta tcaccagttc cggatgatac cactgttcga tctcataaag ggagtctgct
     1141 cctatgataa aatagaactc atgctgggga taagcttccc tgagcaatgt taatgtctgc
     1201 gctgtatagg tgtttccttc cctttttaat tcaaaatcgg aatatacaaa atagggaact
     1261 gacgcaattg ccagcttaac catatcttcc cgttcttttc cctgcgtcac cagacggctc
     1321 ttcttgtgag gaggatggcc ggatggcata aaccagatct catccagttt aaactgctca
     1381 tatgcctgtt tcccaagggc aagatggcca ttatggacag gatcaaaggt tcctcccaaa
     1441 attcctattt ttgccatgtc aaacctctgc gatcatctgt gattaaggta atacgatctt
     1501 tctctttgtc tcatcctttg ccggacgata cagtacgatc tttcttccga tcacctgtac
     1561 tacttctgaa tgagtacgct cgcccagtac ttctgcgatc gatctgccat catccagaca
     1621 gttctttaat acagtgatct ttacaagttc tctggcttct aatgcttcag aaacagcctg
     1681 tgtcacttct ggtgtaaggc tggacttacc gatctggaag atcggatcca ggttcattgc
     1741 aagacctttt aaataggatc tctgcttact tgtcattggt ttctccttta tctatataaa
     1801 cgaatacgat caatagtcac attttatttg taataatcaa actccagtcc atacatacgg
     1861 acagtgtcac cttcactgat accggccttt tcaaggtctt ctaaaatacc ggtgttcttt
     1921 aagaacttct ggaagaaatc aaatcccttc tcagagtcaa ggttggtata accaagcatt
     1981 ttttcaattc ttggtccttc tactacataa gcaccatcat ctgctcttgt aacagtgtaa
     2041 ggcaggttac ggtcgccctg gtactcgatc tcaaattcct tctggaaaac aaccggtgcg
     2101 gaatctactg tctttaacag gtcatttaca tgccataaca gctcttttac gccctgtctg
     2161 ctgacagcgg agattgggaa taccttaatg ccctttggct caaattctgc ctttaatctg
     2221 gcaatgatgt cgttggagtc atcttctgag aaacaggcat ccattttgtt agctgcgatc
     2281 acctgaggac gctttaaaag ttccggatta tatgcttcca gttctttatt gatcgtcaga
     2341 atgtcctcta acggatctct tccttctaca gatgcaccgt ctactacatg gactaatact
     2401 ttggtacgct caatgtggcg caggaaggaa tgtcccagtc ctactccctc agaagctccc
     2461 tcgatcagtc caggaatatc tgccataaca aaacctgcgc catcacccag atctaccaca
     2521 cccagatgtg gatttaaggt ggtaaaatga tagtttgcga tctcaggtct tgcgttgctg
     2581 actacagata ataaagtaga ttttccaaca tttggaaatc ctaccagacc tacgtctgcg
     2641 atcaccttaa gctccagggt gacccacagt tcctgtccag gctgtccagg ctgtgcatac
     2701 ttgggaacct gcatggtgga ggttgcgaaa tgcatgttgc caaggccgcc ttttccgcct
     2761 tttaaaataa cttctctttt attatcaccg gacatatctg caatgacttt tccggattca
     2821 aaatctttaa ttacagtacc ttctggtact tttacgatca gatcatcggc atctgctccg
     2881 tgacagcggc gctttccgcc ttcctgtccg tcttttgcca catatttatg gatgtgacgg
     2941 aaatctacca gtgtgttttt tccggtatct acctggaaaa taacatcgcc gcctcttccg
     3001 ccgtctccgc cgtcaggacc gccattagga acatacagtt ctcttctgaa agaaacatga
     3061 ccgtctccgc ctttaccgga ctttataaat acttttgctc tatctgcaaa cattatacag
     3121 cacctcctgt tggtgttgaa tgtgaagatc cccagtaacc aaactatgct tttaccactg
     3181 aggatacaga aaaaaggggc tccatacata ttcgtatggg gccctcagaa ttactcgttg
     3241 attgctcttg ggtaaacaga aacctgtttc ttgtctctgc cctttctttc aaacttaaca
     3301 actccgtcta ctaaagcaaa taaggtatcg tcgccgccct tacctacgtt gttgccagga
     3361 tgaatatgtg taccgcgctg tctgtacagg atgtttcctg ccagtacgaa ctgtccatca
     3421 gctctctttg cgccaagtct cttggactcg gaatcacggc cgttcttagt agaaccaaca
     3481 ccctttttat gagcgaataa ctgaaggttc atctttaaca tgtcttacac ctccttaaat
     3541 cggatattaa tgtatgattt tccataatca ttctcaatat tctgaagtcc cagaacaaga
     3601 gaattcataa ggagctgtga ctcagggctt atatctgaag taaagcggaa gtcaaactga
     3661 acgtcttcct gtcctgctat cccttcaaat ccgtcatccg taaatgtctc tactgaatta
     3721 taaaaattaa tagccagtgc agaaactgct gcgcagatga tatcctcacc ttcttcagca
     3781 taacctgcat gccctttaat cacgatcccg cagtaaagat cattgcctga atctgtggat
     3841 ttcttaaata ctgtcgtctg gatcatacca tttacaatta agcgttgatc ttttcgatct
     3901 taacctgtgt aaagagctgt ctgtggccgt tctttttgtg atagccagtt tttctcttat
     3961 acttgtaaac gataactttc tttcccttgc cttccttctc aactgttgct gtaacagttg
     4021 ctccggctac ggttgggcat ccaatctgca gttcgccgtt gttaacagcc agcacctggt
     4081 caaatgttac ggtctcgcct gcgcctgcgc ccagtctttc taccttaatg acatcgcctt
     4141 ctgctacctt gtactgcttt ccgcctgttg caataatcgc gtacataaaa aggcacctcc
     4201 tatgttcatt actcgccagc ttcggtgttt ctttgcgaaa tcttttacct cgctgtgcgg
     4261 catactcaag tataatagca cccgactttt caaatgtcaa ggacattttt aatttttttg
     4321 aactgctttt tttggactgc gccaatatat tttatagggc tgtttttatc ctctcttcca
     4381 ggttccagga gaaagaagga caagtaccgg gaacagctcc agtcttcctg ccagcatatc
     4441 aaagatcagc acggattttg acagatagga catcatggag aaattggaag caggtcctac
     4501 aacggaaagt cccggtccaa tattgttgaa gtttgctgct actgctgtaa agctggtggt
     4561 aaaatcgtaa ttatccaggc tgacaataag gaccgacgct acaaacagca taaaatatac
     4621 gatgatatag cagtttaaaa cacgaagagt atcatttccg atcacttttc cttcatattt
     4681 taagaccttt acacttcttg ggtggatcat agtagagatt tcccgtttgg cttctttaaa
     4741 tgcgatcata atacgtgaaa ccttcattcc gccgcctgta cttccggcgc aggcaccgat
     4801 gaacattaac agtacgataa tgcattttga aaattccggc catttatcaa aatccacagt
     4861 ggaataacct gtagttgtaa taatagaagc aacctggaat aatgcgtgat gggcagccag
     4921 gaaaagggac gggaacatgg agcggatatt aacagtaatc agtaccactg cgatcaggat
     4981 gatcgccaga tatccacgca tttcctcaca cttaaatgct tcttttggtt tgcggatgag
     5041 aagcaggtaa tatacgttaa agttaatgcc aaaaaggatc atggcaatac ttaaaactgc
     5101 ctgctggtat accgtatatc ccgcctggcc gctgtttcgg caggcaaaac ctcctgtacc
     5161 tgctgcgcca aggctcatac agatagtatc aaatacaggc atttttccaa ttaataagaa
     5221 aacagccgtt gccactgtaa tacagaaata gatgatatac aaaagttttg cagtaatacg
     5281 gatcttcggt accagtttcc ctacagaagg tcccggactt tctgctctca tgatgtgcat
     5341 atcatagtct cctgccatgg gaagaatagc aaggataaat accagaacgc ccatgcctcc
     5401 gatccagtgg gaaaaacttc tccagatcag gctggctttt gacagcttct ccacctctgg
     5461 aaggatacta gaaccggtag tagtaaaacc ggacacaatt tcaaacaaag catcaataaa
     5521 atgaggtatc tccccgctta acacaaaagg aaggcagcca aaaaagctta gaaccagcca
     5581 gcctaaggaa accagtacaa agccttccct tgcaaaaaac atccttacct ttggcttttt
     5641 tctggtacat aaaaaaccaa gaactgcaca tacaactgct acccctgcgt aacagctgat
     5701 aattccttct ttataaacga ctgctgtcag gatagggatc agcatcagcg ccgcttctat
     5761 attcatgatc cagcctaaca tataaataat aatactatga ttcattgcct aaaccccttt
     5821 actttaatat gtcggtcaga tcattcaggc ctgtatgggt cgtaacaatg atcacggtat
     5881 cgcccgcttc aatcgtatct ttgcctcgag gggtaataat acagccgctg cggttgatac
     5941 aggctactaa cagatgattt ttcagttgca gcctttccaa cggaatacca agaagagggg
     6001 atccttcttt tacgcggaat tccaacgcct ctgccttgtc agctacgatc ttataaagag
     6061 tttctacatt gcttcccata gaattctgca ttgctcttac atactgaagg atcaggtctg
     6121 cagtaagcat tttcggctgg atcaggcttc caagattcag ggaattgatc acactttcaa
     6181 aagcaatcct gtttaccttc gtgatcagct ttgcattgga tttgctggct gcatacaggg
     6241 aaagcatgat attttcttca tcaaagcctg ttaaagaagc aaaagcctct gtctgaagga
     6301 taccttcttc caataaaacc tgctggtcag aaccgtcacc gtggatgatc atggcatttg
     6361 gcagcagttc acttaactgt ctgcaccgtg cttcatcctg ctcaatgatc ttgatcttga
     6421 tctttgtatc cgccagctgg ttgcaaagat aatatgtcag ctttccaccg cctacaaaca
     6481 tggcagtttt tacggagttg tttacgattc cggctttctt aaagaagtcc gcactgtctg
     6541 tatgggatgc gataaatgag atcttgtctc ctttctgcat cacaaaatta ccatccggga
     6601 tcacaacgtc atccccgcgc tctacagcac agatcaacac attgctcttt aacttattta
     6661 ccacatccat gacctttaac tggtctaaat tggaatgatc cgggatgaga agctttaaca
     6721 gctcgatcct tcctttagca aacggctcga tcttaatagc agacggaaaa cgcagaagtc
     6781 ttgcgatttc tctcgcagct gccagttccg ggttaatggc catggaaagg tttaattcct
     6841 ctctgatata gccgatctcc gcggaatatt caggattacg gatacgtgca atggtatggc
     6901 agttacctgc ttttttggcg atcagacagc agagcatgtt caattcatct gagttagtgg
     6961 tggcaatcat cagatctgcc tcctggatgc ctgcctccat ctgtacctga taaatcgcac
     7021 catttcctgt tacactcatc agatccacat tgtcagaaat atttttcaat gcctcatcat
     7081 cggtatcgat cagtgtgatg tcatggtttt ctctgtttaa ctgttctgcc agagtagttc
     7141 ctactttacc gcagcctaca attatg
//
LOCUS       DBMDUA010000145         7112 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4499_length_7112_cov_2.880759, whole genome shotgun sequence.
ACCESSION   DBMDUA010000145 DBMDUA010000000
VERSION     DBMDUA010000145.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4499_length_7112_cov_2.880759"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="ACZPCL_10230"
     CDS             <1..1063
                     /locus_tag="ACZPCL_10230"
                     /inference="COORDINATES: protein motif:HMM:NF015515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HIR1162881.1"
                     /translation="VFTSGTLSCMVTGSVARPVNDALKVPHEKAAFIVHTTSTPWCVL
                     FPFSGWLAAMAGYLVTGGVPEDQSVSVLFQSLFMNFYCILAILLVLVLALTQKDFGPM
                     AKAEARAATTGELDDPIHKKADSDTSKAVSGFTPRAINLLLPVGTLIAVMFAALIITG
                     NGNLMNGSGMKALIWAVIIALLVASVLCVAEKVFTVSQVIDEIFKGMSGMLPITFVLL
                     FGFTMGNVVKSLDTGTYLSSIFQQFLSPALLPALTFLIAVLISFATGTSMGTMAIMAV
                     IALPMAYSMGNSIPLVAGAMFGGSIFGDHASPISDTTIMTCSTTGCDIIDHIKTQFPY
                     VCSIAVISFVLYVIAGFIM"
     gene            complement(1173..2966)
                     /locus_tag="ACZPCL_10235"
     CDS             complement(1173..2966)
                     /locus_tag="ACZPCL_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HIR1162882.1"
                     /translation="MVNLTIDRLPVSVKEGTTLMEAAESIGIHIPHLCYWKGLNEIAA
                     CRVCVIELEGRDHLITACNTKVEEGMVIYTNSPKVRLDRRLTVQLILSQHDCRCATCV
                     KSGNCSLQKIATDLNVQDGLFEERLEKQPWDKTFPLIRQSEKCIKCMRCVQVCDKIQS
                     LNIWDLEGTGSRSTINVTGNRTINEADCSLCGQCITHCPVGALHERDDTEKLWRALAD
                     PNKTVVVQVAPAVRAAWGEDLGFTREEATIGKIFDALKKMGADYVFDSCFTADLTIME
                     EANELLVRLGKGELKDRPMFTSCCPGWVRFVKSEFPQFVKQLSTAKSPMQMFGAVMKS
                     YFAEQIGKKPEDIFSVAIMPCLAKKGEANMELFYKEYAGHDTDCVITTRELIRMIKGS
                     SIITDRLTDIEPDRIFHDASGAGVIFGATGGVMEAALRTAYYAIMGENCAPDAFKNVR
                     ASSQETGVTEAEFELNGQILRIAAVSGLGNTRNLLKAIERGEKHYEFVEVMACPGGCV
                     GGGGQPQHDGMEMAFERGKNLYFLDENADIRRSHENPDVKALYDNYFEQPLSHKSHML
                     LHTDHNKWQMPPSPQRNRNGYVINPNAKLQG"
     gene            complement(2960..4792)
                     /locus_tag="ACZPCL_10240"
     CDS             complement(2960..4792)
                     /locus_tag="ACZPCL_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR1162883.1"
                     /translation="MSRLELFTPSKDQLMVESLYKNLEQRIVASPPGICPVDITRAFI
                     EMCHAQTCGKCVPCRVGLHQLKHMITNVLNGDADYEILDLIEETSLSIMQTADCAIGS
                     EAAKMVWRMVHTCRDDFEAHIRNKRCSCAISQPVPCVALCPAHVDIPGYIALVREGRY
                     ADAIQLIRKDNPFPSTCGFICEHPCEARCRRNMVDTSVNIRGLKRAAADLAGKVAPPP
                     CGPSTGKKIAVVGGGPSGLSSAYYLQLMGHQVTVYEMLPKLGGMLRYGIPNYRLPKDR
                     LEEDTDAILETGIQVHYNVKIGEDIKLDELRKVYDAVLITVGASTDKKLGLPNEDAEG
                     ITSAVGFLRNVSLGDCPDLTGQEVAVIGGGNVSMDAVRTAIRLGAKKVSIVYRRRVAD
                     MTALPAEIEAAVAEGVELLTLVAPAGIEVDENGHVSGVRVTPQMISTIKDGRASVIAS
                     GEPEYVIPCQTMIVAIGQNIETKHYEESGIPVSRGKIVTLPNGGFADIPGVFAGGDCA
                     SGPATVIKAVAAAKVTAANIDEYLGYHHEITCNVEIPLPNNADHTPCGRVELSEREAS
                     ERKHDFEGVENCMNKKEIAQESGRCLRCDHFGFGIFKGGREKLW"
     gene            5178..6242
                     /locus_tag="ACZPCL_10245"
     CDS             5178..6242
                     /locus_tag="ACZPCL_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1162884.1"
                     /translation="MGESINIDLQKKVSLTRQELHELETKEKTGKERLIEKLILVLPE
                     ICAVTAILEYMLIPDNSMNRNPYTYLGFLILMMVLYNGYGIAAAILHCKGDKRLYEKF
                     RYRAPLWSALFLLFAGSDYLTLKTGVLTQPFVPCMNFILNAAWTDRAMILDCTIHTLG
                     LLFLGYFAGVSLGLVTGITCGYSEKARYWVDPVIKFLGPIPTSTWIPIMMVIASSLFG
                     GAVFIIALGSWFAVTVASMTGIANVDRDYFEAARTLGASEQQLVFQVALPHAMPSILQ
                     GCTQAMSSSCTAIMIAEMLGVKSGLGWYMNWSKSWASYDKMFAALFVICFIFTVVTKI
                     LDRVKCHVLRWQNGAVKNYG"
     gene            6235..7017
                     /locus_tag="ACZPCL_10250"
     CDS             6235..7017
                     /locus_tag="ACZPCL_10250"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1162885.1"
                     /translation="MDENNKILLKLENISKSFAKVENDEVTHALDCIDLEMRSGEFIS
                     LVGPSGCGKSTILRLVAGLIQPTLGKVTVNEKEITGPSPERGMVFQKPTLFPWLTVEK
                     NIAFPLKMQGRLNDANGKEKVEHMLHVIGLEGFRDDYPGQLSGGMAQRVALVRTLISQ
                     PEILLLDEPLGALDAFTRMNMQDEILSMWREHRQLALMVTHDVDEAIYMGTRVIVMDA
                     NPGRIIADIKIEEEFPRDRSSERFIRYRNMILNQLHFAGRNI"
BASE COUNT         1917 a         1575 c         1646 g         1974 t
ORIGIN      
        1 ggtatttact tccgggacct taagctgtat ggtaacaggt tctgttgcaa gacctgtaaa
       61 tgatgcttta aaagtacctc atgagaaagc agcttttatc gtacatacca cttccacacc
      121 atggtgtgta ttattcccat tcagcggctg gctggcagct atggcaggtt atctggtaac
      181 cggtggtgta ccggaagatc agtctgtatc cgttctgttc cagtccctgt ttatgaactt
      241 ctactgtatc ctggcgatcc ttctggttct ggtactggca ttaacacaga aagactttgg
      301 accaatggcg aaggcagaag ccagagcagc aacaaccggc gaactggatg atccgatcca
      361 caaaaaggcg gactccgata cttctaaagc agtatctggt ttcactccaa gagccatcaa
      421 cctgcttctt ccggtaggta ctctgatcgc agttatgttt gcagcactga tcattactgg
      481 taatggaaac ctgatgaacg gttctggtat gaaggctctg atctgggcag ttatcatagc
      541 cttattagta gcatctgttc tttgcgtagc agaaaaagta tttaccgtca gccaggtaat
      601 tgacgagatc ttcaagggca tgagcggcat gcttcctatt acctttgttc ttctgtttgg
      661 atttaccatg ggtaatgtag ttaagtctct ggatacaggt acttatcttt ccagtatttt
      721 ccagcagttc ttaagcccgg ctcttcttcc tgccttgacc ttcctgatcg cagtcctgat
      781 ctcctttgca acaggaactt ctatgggaac tatggctatt atggcagtta ttgcacttcc
      841 tatggcatat tccatgggca acagcattcc gttagtagca ggagctatgt ttggtggaag
      901 tatcttcggt gaccacgcat cacctatctc cgataccacc attatgacct gttccactac
      961 gggctgcgat atcatagacc atataaagac ccagttccct tatgtgtgca gtattgcagt
     1021 gatcagtttt gtactatacg tgatcgcagg atttatcatg taacgaaaaa atgatataaa
     1081 aaatcttccg gtatggaaaa attccaacac cggaagatct tttgttttta tgattttcgc
     1141 aaatccccca aaagggggtc agatctcaca tcttatccct gtaatttcgc attgggatta
     1201 ataacatatc cattgcggtt tctctgagga cttggcggca tctgccactt gttatggtca
     1261 gtatgcagca gcatatggga cttatggctt aatggctgtt caaagtagtt gtcataaaga
     1321 gcctttacat ccggattttc atgggaacgg cggatatcag cattctcatc caggaaatac
     1381 aggttctttc cacgctcaaa ggccatttcc ataccgtcat gctgaggctg tccgcctccg
     1441 ccaacgcagc cgcccgggca tgccatgact tctacaaatt cgtaatgctt ttcaccacgt
     1501 tcaatggctt ttaacagatt tctggtattt ccaaggccgc tgacagcagc aatacgcagg
     1561 atctgtccat ttaattcaaa ttctgcttct gtaacgccgg tctcctgaga ggaagcacgt
     1621 acatttttaa aggcatcagg tgcgcagttt tcacccatga tggcatagta agcagtacga
     1681 agagctgctt ccataacacc gcctgtagct ccaaagatca caccggcacc ggaagcgtcg
     1741 tggaagatac ggtccggttc aatatcagtg agacggtcag taatgatgct ggaacctttg
     1801 atcatacgga tcagctcgcg ggtagtgatg acgcagtctg tatcatggcc tgcatattcc
     1861 ttgtagaaca gctccatatt tgcttcgccc tttttagcca gacaaggcat aatggcaacg
     1921 gagaagatat cttccggctt tttgccgatc tgctctgcaa aatagctctt cattacagcg
     1981 ccaaacatct gcatcggaga cttggcagta gataactgct ttacaaactg tgggaactcg
     2041 ctctttacaa agcgtaccca gccaggacag caggaggtga acataggacg gtcctttagt
     2101 tctcccttgc caaggcgcac taacagctca tttgcctctt ccataatggt aaggtcagca
     2161 gtaaagcagg agtcaaatac ataatcagcg cccattttct ttaaagcgtc aaagatcttt
     2221 ccaatagtag cttcttccct tgtaaatcca aggtcttcac cccatgcagc acggacagca
     2281 ggagcaacct gtacaacgac ggtcttgtta ggatccgcta aagctctcca cagtttttcc
     2341 gtatcgtcac gctcatgaag ggcgccaact ggacagtggg tgatacactg gccgcagaga
     2401 gaacagtccg cttcattgat cgtgcggttt ccggttacat taatggtaga acgggaaccg
     2461 gtaccttcca gatcccagat attcaagctc tggatcttgt cacagacctg aacgcagcgc
     2521 atacatttaa tacatttttc agactgacgg atcagtggga aagttttatc ccatggctgt
     2581 ttttccagac gttcctcaaa aagaccgtcc tggacattca gatctgttgc gatcttctga
     2641 agagaacagt taccgctttt tacacaggta gcgcaccggc agtcatgctg ggataagatc
     2701 agctgaacgg taagacggcg gtctaagcgc acttttggac tgttggtgta aatgaccata
     2761 ccttcttcta cctttgtgtt gcaggcggtg atcaggtggt cacgtccttc cagttcaata
     2821 acacatacgc ggcaggcagc gatctcgttt aaacctttcc agtagcataa gtgtgggata
     2881 tggatgccga tagattcggc agcttccatc agggtagttc cttcttttac agatacaggc
     2941 agtctgtcga tcgttaaatt taccataatt tctcacgccc tcctttgaag attccaaagc
     3001 cgaaatgatc gcagcggagg cagcgtccgg attcctgcgc aatttctttt ttgttcatgc
     3061 agttttcaac accttcaaaa tcatgttttc gttcacttgc ttcccgttca gacagctcta
     3121 cacgaccgca aggtgtgtgg tcagcgttat ttggcagcgg gatctctaca ttacaggtga
     3181 tttcgtgatg atagcctaaa tattcatcaa tattagcagc agttaccttg gcagcagcta
     3241 cagctttgat aacagtagca ggaccggaag cacagtcacc acctgcaaat acaccaggaa
     3301 tatctgcaaa tccgccgttt ggaagagtaa cgatctttcc gcgggataca ggaatgccag
     3361 actcttcata gtgctttgtc tcgatattct gtccgatagc aacgatcatg gtctgacatg
     3421 ggatcacata ctccggttcg ccgctggcaa ttacgcttgc gcggccgtct ttgatggtgc
     3481 tgatcatctg cggggtaaca cggacaccgg atacatgtcc attttcatct acttcaatac
     3541 cggcaggagc aactaaagta agcagttcca caccttcagc gacagctgcc tcgatctcag
     3601 ccgggagagc tgtcatatca gctacacgac ggcggtatac gatgctgact ttctttgcac
     3661 ccagacggat ggcagtgcgg acagcgtcca tggatacgtt tccgccacca ataacagcga
     3721 cttcctgtcc ggtaagatcc gggcagtccc cgagacttac atttctaagg aaaccaacag
     3781 cagaggtgat gccttctgca tcttcattag gaagtcccag tttcttatct gtagaagcac
     3841 ctactgtaat aagcactgca tcatatactt ttctcagttc gtccagttta atatcttcac
     3901 cgatctttac attgtaatgt acctggatac cggtttccag gatcgcatct gtatcttctt
     3961 ccagacggtc ttttggcaga cggtagtttg gaataccgta gcgcagcata ccgcctagtt
     4021 ttggcagcat ctcataaaca gtgacctgat ggcccattaa ctgcagataa taagcagaag
     4081 agagaccgga aggtccgccg cctacaacag cgatcttctt gcctgtggaa ggaccacatg
     4141 gcggaggtgc tacttttcct gcaaggtcag cagcagcacg ctttaatcca cggatattta
     4201 cagaggtatc taccatatta cggcggcagc gtgcctcaca tggatgttcg cagatgaaac
     4261 cgcaggtaga agggaatggg ttgtccttgc ggatcagctg gatggcgtct gcatagcggc
     4321 cttctcttac cagggcaata taacctggaa tgtccacatg ggccggacag agggctacgc
     4381 agggaaccgg ctgggaaata gcgcagctgc atcgtttgtt gcggatatgt gcctcaaaat
     4441 catcacggca ggtatgtacc atacgccata ccatttttgc agcttcagag ccgatggcac
     4501 agtctgcggt ctgcataatg gaaagggaag tttcctcgat caggtccagt atctcgtagt
     4561 cagcatcacc atttaataca ttggtgatca tgtgctttaa ctggtgaaga cctacacggc
     4621 agggaacaca ttttccacag gtctgtgcat ggcacatttc aataaaggcc cttgttatgt
     4681 caacagggca gatacctgga gggcttgcca cgatacgctg ctccagattt ttgtaaagac
     4741 tctcaaccat cagctggtct ttacttgggg tgaacaactc caatctgctc atatgaatcc
     4801 tcctgaattt ttattgtatg ttcatctgat caggcatctg tcattacaga tattgctgca
     4861 aaaccatgcc tgaaatcctg tgaacacata ttttgtttaa tgataataaa tgtcattaat
     4921 aagtataaca tagaattgta aaaaatgcac tataaaaaat acaaaatgtt tgcaataatt
     4981 tttaacaatc taaaaatttt tggtagcata gatatagtat cgtttgcagt tcttatttct
     5041 gcggttttaa aggatttata gatgatatca ataaatgagt aaaatttttg aaggaaagcg
     5101 attggacaaa ttcccttccg ctcgataaaa taataacaca cagcaagaga tgcgaagatt
     5161 atttacaggg ggaaactatg ggggagagta taaatataga tctgcaaaag aaggtcagcc
     5221 tgacccgtca agaactgcat gaattggaga caaaagaaaa gacgggaaag gaacggctga
     5281 tcgagaaatt aatattggtc cttccggaaa tttgcgcagt gacagcaatt ttggagtata
     5341 tgctgatacc ggacaatagt atgaaccgaa atccgtatac atacctggga tttttgatcc
     5401 tgatgatggt tctttataat ggatacggca tagctgccgc tattttgcat tgtaagggag
     5461 ataagcggtt gtatgagaaa tttcgttacc gtgcgccctt atggtcagcg ctgtttttat
     5521 tgtttgcagg ttctgattat ctgaccctga aaacaggagt tcttacccag ccttttgtac
     5581 cgtgtatgaa ctttatatta aacgcagcat ggacagaccg ggccatgata ctggattgta
     5641 ccatccatac gctgggactt ttatttttgg gatattttgc aggtgtgagc ctgggactgg
     5701 taacaggtat tacctgcggc tattcagaaa aagcccgtta ctgggtagat ccggtgatca
     5761 aatttttagg tccgatccct acgtctacct ggatcccgat catgatggtc atagcatctt
     5821 ctttatttgg aggagcggta tttatcattg cccttggctc ctggtttgcg gtaaccgttg
     5881 cgtctatgac agggatcgca aatgtagaca gagattattt tgaagcagcc cgtaccttag
     5941 gtgccagtga gcaacagctt gtttttcagg ttgcgttgcc tcacgccatg ccttctattt
     6001 tacagggatg tacccaggct atgagcagtt cctgtacagc tattatgatc gcagagatgc
     6061 tcggcgtaaa atcaggactt ggatggtata tgaactggag caaatcctgg gcaagttatg
     6121 acaaaatgtt tgcggctctg tttgtgatct gctttatttt tacagttgta acaaaaatat
     6181 tagaccgcgt gaaatgccat gtgctcaggt ggcagaacgg agcagtgaag aattatggat
     6241 gagaacaaca aaatattatt aaaactggaa aatatttcca aaagctttgc aaaggtagaa
     6301 aatgatgaag tcacccatgc tcttgactgt attgatcttg aaatgagaag tggggagttc
     6361 atcagtctgg tgggaccttc aggttgtgga aaatccacga tcctgcggtt ggtggccggt
     6421 ctgatccagc ctacattagg aaaagtaaca gtaaatgaaa aagagatcac aggtccatcc
     6481 cctgagagag gaatggtttt tcagaaacct accttatttc catggcttac agtggagaag
     6541 aacattgctt ttcctttaaa aatgcaggga aggttaaacg atgcaaatgg aaaagaaaaa
     6601 gtggagcata tgcttcatgt gatcggactg gaaggtttcc gggacgatta tccgggacaa
     6661 ctttcaggag gaatggctca gagagtggca ctggtccgga cactgatcag tcagccggag
     6721 atcttattgt tagatgaacc gctgggggct cttgatgcat ttacacgcat gaatatgcag
     6781 gacgagatcc tttctatgtg gagagaacac aggcagctgg cgttgatggt gacacatgat
     6841 gtagatgaag ccatttatat gggaacaagg gttattgtaa tggacgcaaa tccgggaagg
     6901 atcattgcag atattaaaat agaagaagaa tttccaagag accgctcttc ggagagattt
     6961 atcagataca gaaatatgat tttgaaccag cttcattttg cggggagaaa tatataacat
     7021 catttattaa gtccagcggt gtgatgtcaa gaaaataaat gatttaaaaa tcatacattt
     7081 ttgatatata ccgcaaagac aaaaaacaca ca
//
LOCUS       DBMDUA010000146         7035 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4547_length_7035_cov_2.614508, whole genome shotgun sequence.
ACCESSION   DBMDUA010000146 DBMDUA010000000
VERSION     DBMDUA010000146.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4547_length_7035_cov_2.614508"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2010)
                     /locus_tag="ACZPCL_10255"
     CDS             complement(<1..2010)
                     /locus_tag="ACZPCL_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR1162886.1"
                     /translation="MNSLREWEEAFEQAVDVKKSYDLAKRMEEPKTNPVLGYRTAGSK
                     AEWETGELLLKEMQEMGLQNVQKDKIKVDSWDFHHAVLRYRDKEGKEYEFQLGAYQTD
                     FHTEGFQEFSMVYLGRGTAENYENIDVKGKLVLIDINQREEWWINFPVYQAHLKGAAA
                     LIAVQDQGYGEIHDTSLNAQDIAGPKEAAAFSISQADAAILKEDMGNMEKTGNYTGDF
                     KEIQVLFDAQSTVIRDRESYNITGMIPGEEPEQMIMLSAHYDSYFTGFQDDNAAVAMM
                     LGIARTFIDMGYKPRKTLVFCAMAAEEWGVVDSKYDWSTGAYEQVFTAHPEWQGKVIA
                     DFNFELPAHAHGKKDAVRCTYEYASFMKKATEHIQPSKEAYPEGLEVHFPIQTWSDDF
                     SIAIAGIPSSVNEFSDGEFMETHYHSQFDNEDFYDEPVYRFHHNLYGKLVLAFDHTAV
                     VPMDFERLFAAVEDSVDRKLCEKTEANETVLLERLKKAKELGRQLYDQIQKINETYKN
                     LLETGKCEEAKAMAAEYAALNSSLLYIFRKEQDYFVRLNWHDDVLFPQQGVGENLKAI
                     YKALGCLKEDDPEGALEAVYEIDNNRYAFLFDREVFRYFTEYVLNQPADRLKWGYGRI
                     IHHENLYDLVVSLKEKNKLQKAGERPDLEEEYSKRHHGGYLGERSR"
     gene            complement(2171..3166)
                     /locus_tag="ACZPCL_10260"
     CDS             complement(2171..3166)
                     /locus_tag="ACZPCL_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162887.1"
                     /translation="MAYSKKRLIAAAMAGVLAMMSAFPGYAASRKKITSVSVSIKADI
                     EPETDFGQEIIEIESSSNRYSVDGYEILNEGFYWTEDMTPEIRITLTADDDYYFTALT
                     KDKVTLKGGAEFKKSTRQQSSSVLLLDVTLSSLQNSLKDMEGITLSDNGIATWPAIST
                     AGSYEVRVYREGKIVGTSLETNTNSANCRERMMKPNETYMVKVRAVNKYDPTVKGDWT
                     ESNSVYISGEKVAQFKADPNASNVNTASGTTGEWKQEADGRWWYRRADGTYPANKWEE
                     LGGKWYFFDENGYMKTGWIDWEGKSYYCSENGDMLTNCMTPDNYLVGEDGAWIAQ"
     gene            complement(3379..4938)
                     /gene="rny"
                     /locus_tag="ACZPCL_10265"
     CDS             complement(3379..4938)
                     /gene="rny"
                     /locus_tag="ACZPCL_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR1162888.1"
                     /translation="MLQIIIPVLIGAVIAAIIAGFIAHSAGRSYERKQYDSKVGSAEE
                     KAREIIDEALKTAETKKREALLEAKEESLKTKNELEKETKERRAELQRYEKRVLSKEE
                     NVEKKADALEKKEADLVRRENILGERTAEVEAQYEQGIQELERISGLTSEQAKEYLLK
                     SVEEDVKHDTAKLIKELENKAKEEADKKARDLVVTAIQRCAADHVAETTVSVVQLPND
                     EMKGRIIGREGRNIRTLETLTGVELIIDDTPEAVVLSGFDPIRREVARIALERLIVDG
                     RIHPARIEEMVEKAQKEVENNMREEGEAACLEVGIHGIHPELVKLLGRMKFRTSYGQN
                     ALKHSIEVAQLSGLLASELGVDVRLAKRAGLLHDIGKSVDHDMEGTHVQLGADLCRKY
                     KESAVVINAVESHHGDTEPTNLISCIVQAADTISAARPGARRETLETYTNRLKQLEDI
                     TNSFKGVDKSFAIQAGREVRIMVIPEQVSDDDMVLLARDISKRIEDELEYPGQIKVNV
                     IRESRVTDYAK"
     gene            complement(5079..5684)
                     /locus_tag="ACZPCL_10270"
     CDS             complement(5079..5684)
                     /locus_tag="ACZPCL_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR1162889.1"
                     /translation="MIVKAVVPVDKRKCKVFLEGDFAFVLYKSEAARFHIEEGNDLPA
                     KTYEMIEEEILLKRARDRALYLLQSQGRTQAEMIKKLKDDGYPQSVTERVLSFLQEYH
                     FIDDNAYTENYIHVNKGRKSKRQITYELQQKGVDRDQIRQMLEENPVDEEETVRALLK
                     KKTGGRIPEDKKEIQKLAAFLGRKGFSFEVISRVLRDVADY"
     gene            complement(5681..6832)
                     /gene="recA"
                     /locus_tag="ACZPCL_10275"
     CDS             complement(5681..6832)
                     /gene="recA"
                     /locus_tag="ACZPCL_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR1162890.1"
                     /translation="MGKEEKMSVNRDMNKEEKLKALDAALTQIEKAYGKGSVMKLGES
                     GVNMNIETVPTGSISLDIALGLGGVPKGRIIEVYGPESSGKTTVALHMVAEVQKRGGI
                     AGFIDAEHALDPVYAKNIGVDIDDLYISQPDNGEQALEITETMVRSGAVDIVIVDSVA
                     ALVPKAEIDGEMGDSHVGLQARLMSQALRKLTAIISKSNCIVIFINQLREKVGVMFGN
                     PEVTTGGRALKFYSSVRMDVRRIETLKQGGEVVGNRVRIKVVKNKIAPPFKEAEFDIM
                     FGKGISKEGDILDLAVKENIVEKSGAWFAYDGSKIGQGRENAKQYLLSHPAICEEIEA
                     QVRVKYNLPGAEEAEAQAREAAKKEEELTGSILPEGTEEELMAGEAEEE"
BASE COUNT         1695 a         1800 c         1273 g         2267 t
ORIGIN      
        1 acgtgaacgc tcacctaagt atcctccatg atgacgttta ctatactctt cttccagatc
       61 cggtctttct cctgctttct gcagtttatt cttttccttt aagcttacta ccagatcata
      121 caggttttcg tggtggataa tacggccata gccccatttt aaccggtctg ccggctggtt
      181 taatacatac tctgtaaaat aacggaatac ttcccggtca aacaggaagg cataccggtt
      241 gttgtcgatc tcataaacgg cttccaaagc gccttccggg tcatcctctt ttaagcagcc
      301 gagagccttg taaatggctt tcaggttttc ccctactccc tgctgaggga acagtacatc
      361 atcatgccag tttaagcgga caaaatagtc ctgttccttg cggaagatat aaaggaggga
      421 gctgtttaag gctgcatatt ctgctgccat ggcttttgcc tcctcacatt ttcctgtttc
      481 cagaagattt ttgtaagttt cattgatctt ctggatctgg tcatataact gacgtcccag
      541 ctcttttgcc tttttcaggc gttctaacag gaccgtctca tttgcctctg tcttttcaca
      601 cagctttctg tccacgctgt cttccactgc tgcaaacagc cgttcaaagt ccataggtac
      661 tactgctgta tggtcaaatg ccagcaccag ctttccataa agattgtgat ggaagcggta
      721 aaccggctca tcatagaaat cttcattgtc aaactgggaa tgataatggg tctccataaa
      781 ctctccatcg ctaaactcat ttacagagga tggaatacct gcaatggcaa tggaaaaatc
      841 atcggaccag gtctggatgg ggaagtggac ttccaggcct tccgggtatg cctctttaga
      901 cggttggata tgttcggttg ccttcttcat aaaggaagcg tattcatagg tacagcggac
      961 tgcatccttc tttccatggg catgggccgg aagctcgaag ttaaagtctg cgatcacctt
     1021 tccctgccac tccggatggg cggtaaatac ctgttcgtag gcaccggtgg accagtcata
     1081 tttggagtct accacacccc actcttctgc tgccatagca cagaatacaa gagtttttct
     1141 tggcttatat cccatatcga taaaggtcct tgcaattcca agcatcatgg caacagctgc
     1201 gttgtcatcc tggaaaccag taaaataaga gtcataatgg gcagacagca tgatcatctg
     1261 ctccggctct tctcccggga tcataccggt aatgttataa gactcccggt cacggatcac
     1321 tgtagactgg gcatcaaata atacctggat ctccttaaaa tctcctgtat aatttccagt
     1381 tttttccata tttcccatgt cttcttttaa aatagctgca tctgcctggg aaatggagaa
     1441 agcagccgct tctttggggc ctgcaatgtc ctgggcattt aaggaagtat catggatctc
     1501 cccatatccc tgatcctgga cagcgatcag cgcagctgct ccctttaaat gagcctggta
     1561 aacagggaaa ttgatccacc attcttctct ctggttgata tcgatgagca ccagtttgcc
     1621 ttttacatct atattttcat aattttccgc tgtgccacgt cctaagtaga ccatagaaaa
     1681 ttcctgaaaa ccttctgtat ggaaatcggt ctggtaggca cctaactgaa attcgtattc
     1741 ttttccttct ttatcacggt aacgaaggac tgcgtgatga aagtcccagc tgtccacctt
     1801 gatcttgtcc ttctgaacat tctgaaggcc catttcctgc atttctttta aaagcagttc
     1861 gcccgtctcc cactctgctt tagagccggc tgtgcggtat ccaagcaccg gattggtctt
     1921 tggttcttcc attctttttg caagatcgta agattttttt acatcaacag cctgttcaaa
     1981 ggcttcttcc cattccctta aactgttcat tcttctgtcc tctttcttct atgtactctt
     2041 ctaatctctg tatgagaaca catctatctc aaatgctgca tttataataa cacaaaataa
     2101 gccgctgcaa aatgtctttt tacactttgc agcggcttaa acttcttaaa taatatgttt
     2161 tcccgaaaac ttactgggcg atccaagctc cgtcttcacc taccagataa ttgtccggtg
     2221 tcatgcagtt ggtgagcata tcaccgtttt cagagcagta gtagctcttt ccttcccagt
     2281 cgatccagcc ggtcttcata taaccgtttt catcaaagaa gtaccacttt ccgcctaatt
     2341 cttcccactt atttgcagga taggtgccgt ctgctctgcg ataccaccat cttccatccg
     2401 cttcctgttt ccattcaccg gtagtaccgg aagctgtatt tacattggat gcattgggat
     2461 ctgccttaaa ctgtgctact ttttctccac tgatataaac gctgtttgac tcagtccagt
     2521 caccttttac agtaggatcg tacttattga ccgcacgtac ctttaccata taggtttcat
     2581 ttggtttcat catacgttcc ctgcagttag cggaattggt attggtctcc aaagaagttc
     2641 ctacgatctt tccttctctg tatacacgaa cctcataact tcctgctgta ctgatagccg
     2701 gccatgtagc aattccatta tctgataaag taattccttc catatctttc aagctgttct
     2761 ggagagaaga aagggtaaca tccaacagca aaacagagct ggattgctgt ctggtggact
     2821 ttttaaattc cgcgccgccc tttaatgtaa ctttatcctt tgtaagggct gtaaaataat
     2881 aatcatcatc tgctgtcagg gtgatccgga tctccggtgt catatcttct gtccagtaaa
     2941 atccttcatt taagatctcg taaccatcca cactatagcg gttgctgctg ctttcgatct
     3001 caatgatctc ctgtccaaaa tcagtctccg gttcaatatc cgctttaatg ctcactgata
     3061 cagatgtgat cttttttctg ctggctgcat aacctggaaa agcactcatc attgcaagta
     3121 cgcctgccat tgcagcagcg atcagtcttt ttttgctgta tgccattttt attcctcctt
     3181 tttctcacaa accatctatg catcaaaaca aatttttaat gcacacacct gttcaaacag
     3241 gtttctctct ctactctaag ggtatctgtt ttcccttata ctgtcaataa agttttcctt
     3301 acgattttat cagaaagttt aaaggaaatg gggttaaaaa aagctccatc gcaatgatga
     3361 agctttttca tatgattttt acttagcata gtccgttact ctggattcac ggatcacatt
     3421 gactttgatc tgtcccggat attccagttc gtcttcaata cgtttggaaa tatctcttgc
     3481 cagcaatacc atatcgtcat cgctgacctg ttcaggaata accataatac ggacttcccg
     3541 tcctgcctga atagcaaaag acttatctac gcccttgaag gaatttgtaa tatcttcaag
     3601 ctgtttcaat ctgtttgtat atgtttccag tgtttctctt cttgcaccag gtcttgcagc
     3661 agagatcgta tctgcagcct gaacgatgca ggaaataaga ttggttggtt ctgtgtctcc
     3721 atggtgagac tctacagcgt tgataacaac tgcagactcc ttgtactttc tacaaagatc
     3781 tgcccccagc tgtacatgag taccttccat gtcatggtca acagactttc caatatcatg
     3841 gagtaagccg gcacgttttg ccagacgtac atccacaccc agctcagaag ctaaaagccc
     3901 tgataactgt gctacttcga tcgaatgctt taatgcattc tgaccatagc ttgttctgaa
     3961 cttcatccgt cctaacagtt ttaccagttc tggatggatg ccatggatac caacttcgag
     4021 gcaggcagct tcgccctctt ccctcatatt gttctccact tctttctgcg ctttttctac
     4081 catctcttcg attcttgccg gatggatacg gccgtccacg atcagacgtt ccagtgcaat
     4141 tcttgctact tccctgcgga tcggatcaaa tccggataaa acaaccgctt caggagtatc
     4201 atcaatgatc agttccacac ccgttaaagt ctcaagagta cggatattac gtccctcacg
     4261 accaatgatg cgtcccttca tctcatcatt tggaagctgt actacagata cagtcgtctc
     4321 agccacatga tctgctgcac accgctggat ggcagttaca actaaatctc tggctttctt
     4381 atcagcttct tcttttgcct tgttctcaag ttctttgatc agctttgctg tatcatgctt
     4441 tacgtcttct tcaacagatt ttaaaagata ttcttttgcc tgttcggagg taagaccgga
     4501 aattcgttcc agttcctgta ttccctgctc atactgggct tccacttctg cggtacgttc
     4561 accgagaatg ttttcccttc tgaccagatc agcctccttc ttttcaaggg catctgcctt
     4621 tttttcaacg ttttcttctt tgcttaatac acgcttctcg taacgctgaa gttctgctct
     4681 tctttccttg gtttcttttt ccaactcatt ttttgtcttt aaagattctt ccttggcttc
     4741 caggagagct tctcgcttct ttgtttctgc agtctttaat gcttcatcta ttatctctct
     4801 ggctttttct tctgcacttc ctactttact gtcatactgc ttgcgctcat aggaacggcc
     4861 tgcagaatgt gcaataaaac cagcaataat agccgcgatc accgcgccga tcaaaacagg
     4921 aattatgatc tgcaacacag gagcacctcc ttatatttag ttttcattgt acgaaataca
     4981 acatattaat tttatacttt tttcagaatt gtgtcaagta tattcctctt attttcccgt
     5041 tgtaccgaca acataaacaa aagacagccg ggtcttcttt aataatctgc cacatccctc
     5101 aaaactcgac tgatcacctc aaaggagaag cctttccgtc ctaaaaaagc tgccagcttc
     5161 tgtatctctt ttttatcttc cgggattctt cccccggtct tcttttttaa aagggcacgg
     5221 actgtttctt cctcgtctac aggattttct tctaacatct ggcggatctg atcccggtct
     5281 actccctttt gctgcagttc ataggtgatc tgccgtttgc tctttcttcc tttatttaca
     5341 tggatataat tttctgtata ggcattgtca tcaataaagt ggtattcctg aagaaatgaa
     5401 agtacacgct ccgtaactga ctgtggataa ccgtcatctt ttaatttctt tatcatctcc
     5461 gcctgtgtac ggccctgtga ctgaagaaga tataatgccc ggtccctggc tcttttaaga
     5521 agtatttctt cttcgatcat ttcatatgtt tttgcaggaa gatcatttcc ctcttctata
     5581 tgaaagcggg ctgcttcgct tttatataaa acaaatgcaa agtctccctc caggaagact
     5641 ttgcattttc tcttatctac aggcacaact gccttaacga tcattcttct tcagcctcac
     5701 cagccatcag ctcttcttct gtgccttccg gaagaatgga accggtcagt tcttcctctt
     5761 tcttagctgc ctctcttgcc tgtgcctctg cttcttcagc acctggcaga ttatacttta
     5821 cacgtacctg ggcttcgatc tcttcacaga tcgcaggatg agaaagaagg tactgctttg
     5881 cgttctcacg tccctgtccg atcttggagc catcataagc gaaccatgca ccgctctttt
     5941 ctacaatatt ctcttttaca gcaagatcta agatatcgcc ttccttggaa attcccttac
     6001 caaacataat atcaaattct gcctctttaa atggcggagc gatcttattc tttacaacct
     6061 ttatgcggac acggtttcct actacttcgc cgccctgctt taatgtctcg atcctgcgca
     6121 catccatacg gacggaagaa tagaacttaa gggcacgacc accagtagta acttccggat
     6181 ttccgaacat aacgcctact ttttcacgca gctggttgat aaagataacg atacagttgg
     6241 acttgctgat gatggcagtc agttttctta atgcctggga catgagacgg gcctgaagac
     6301 ctacgtggga gtcacccatc tcaccatcga tctcagcttt tggaaccagg gctgctacag
     6361 agtccacgat cacaatatct actgcaccgg aacgtaccat agtctctgtg atctctaaag
     6421 cctgttcccc gttatccggc tgtgaaatat acagatcatc aatatctaca ccgatattct
     6481 ttgcatatac aggatctaag gcatgttctg catcgataaa gcctgcgata ccgcctcttt
     6541 tctgtacctc tgctaccata tgaagggcaa cggtagtctt accggaagac tcaggaccgt
     6601 acacttcaat aatccttccc tttggaacac ctcccagtcc taatgcaata tcaagactga
     6661 tggaaccggt aggaactgtt tcaatattca tatttacacc ggactcacct aatttcataa
     6721 cggagccctt gccgtatgcc ttttcaatct gagtcaaagc agcatctaat gcttttaact
     6781 tttcctcttt attcatatct ctattcacgc tcatcttttc ttctttgccc atgttttcct
     6841 gctactaaaa acatatacac ctgtgtatat gctagatgat ccgctacaat ctcacgattt
     6901 gtagttactg cttcaatgtg tatgctttgg cggtcgcaag caacacgcta ctcacaagtt
     6961 cttgtacact ccacagtcgt aaattcccaa ctaagccatt ggtacatacc tatcaattca
     7021 tttcattgat agata
//
LOCUS       DBMDUA010000147         7023 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4559_length_7023_cov_2.582491, whole genome shotgun sequence.
ACCESSION   DBMDUA010000147 DBMDUA010000000
VERSION     DBMDUA010000147.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4559_length_7023_cov_2.582491"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="ACZPCL_10280"
     CDS             <1..561
                     /locus_tag="ACZPCL_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR1162891.1"
                     /translation="SGRNVISRENGSGPGNDMGRPEYGGNPYEGQAAQNQAAIKKTGR
                     KQEREDGIRRSQRLLLTWLIENPALFDKIKGIITADDFVEDLYHQVAVMVFEGHEAGN
                     VNPAGILSRFINDEDQYKEVAALFNASLKESLNNEEQKKAFAETVMKVRKNSLDTASR
                     NAKDIAQLQEIIKQQAALKQLHISLD"
     gene            578..1657
                     /gene="rpoD"
                     /locus_tag="ACZPCL_10285"
     CDS             578..1657
                     /gene="rpoD"
                     /locus_tag="ACZPCL_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR1162892.1"
                     /translation="MERTRREESLSFKEKMGRLLADARAGKRQLEYRDIFRYFRPKEL
                     KEEELDRVLDILAENQVQILDEGEEQDAGESEPVSEEKTGQADPVCLYLKEIGKISLL
                     TPEEELMLAKEVRAGSREAKEKLARANLRLVVSIAKHYAGRGMAFLDLVQEGNLGLMR
                     AVEKFNYEKGYRFSTYATWWIRQSITRAIADQARTIRIPVHMVEVINRVMRSSRRMLQ
                     ELGREPTTQEIAERLHMTSKKVEEVLNMAQEPVSLETPVGEEEDSHLVDFIQDEKMSF
                     LQDEASFVFLHEQLMEVLKTLTPREQQVLSLRFGLYDGQPRTLEEVGKQFHVTRERIR
                     QIEDKALRKLRHPSRSRKLKDYLES"
     gene            1667..2443
                     /locus_tag="ACZPCL_10290"
     CDS             1667..2443
                     /locus_tag="ACZPCL_10290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1162893.1"
                     /translation="MTEKIQETEIKKTRLRLSERLKLVASFVPEGSRIADIGTDHGYV
                     PIYLAEIGKIKSALAMDVRKGPLARADEHIEEYRHDVGDAAISIETRLSNGLEKLQAK
                     EADTVIIAGMGGELEISILEAGKQLWSSIRHWIFSPQSDLEKFRRYLKENGFFIENEA
                     MILDEGKFYTVIKAGFGTNEKDACVCKTLPEYRFGAVLIKRRDAVLKQYLLKEEKRVE
                     GILKGFAGKSEEELTAGQAAACKALKEELSCIREAQDEMQ"
     gene            2430..3239
                     /locus_tag="ACZPCL_10295"
     CDS             2430..3239
                     /locus_tag="ACZPCL_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIR1162894.1"
                     /translation="MKCSEVIEILGKLAPESCACDWDNPGLLAGRSDKEVKKIYIALD
                     ATDQVVDAAIKAGADMLLTHHPLIFKAIKKVNDQNFITRRLVKLIQADISYYAMHTNF
                     DAAPGCMADLAAERLSLKDTEPLEAMGDMEINGNSVAYGIGKTGNLAEKLTVKALAEK
                     VKQAFDLPFVLVYGEELMDMEVEKVALCPGAGGSVIEEAIHAGAKALVTGDISHHQGI
                     DAAARNMAVIDAGHYGMEHIFIQYMAGYLKENLPVEMEIVTAPSAWPTVLL"
     gene            3285..4937
                     /locus_tag="ACZPCL_10300"
     CDS             3285..4937
                     /locus_tag="ACZPCL_10300"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase family protein"
                     /protein_id="HIR1162895.1"
                     /translation="MVQLKINTQIKEYPDDITWQVIADEYQSGYSDEILLVQVNGKLQ
                     ELQEKIREGEVQFITARQKPGISAYQRSATLLMLKAFYAVAGPENVEKVIVDFSIGKG
                     FFVEARGSFTLNQELLDQVKAKMQEYVDQEIPILKRSISTDDAIERFHKHRMYDKERL
                     FLYRRVSRVNVYSIDGFEDYFYGYMVPNTKYIRYFNLKLYEYGFVLMLPSMLAPTVLP
                     EFAPLPKLFHTLADSSEWGKRLDLETVGALNDKIAAGDMSHLILIQEALQEKRIAEIA
                     AQIAERKTAKIVMIAGPSSSGKTTFSHRLSVQLEAIGLKPHPIAVDNYFVNRVDSPRD
                     ENGNYNYEILECLDVEQFNQDMQALLRGERVELPYYNFKKGEREYKGDFLKLGKDDIL
                     VIEGIHCLNDRLSYSLPRESKFKIYISALTQLNIDEHNRIPTTDGRLLRRMIRDARTR
                     GASAQDTIRMWPSVRKGEEENIFPYQEEADAMFNSALIYELAVLKQYVEPLLFGIPKD
                     CPEYTEAKRLLKFLDYFIGVSSEDIPKNSILREFIGGSCLDV"
     gene            5031..5384
                     /gene="rnpB"
                     /locus_tag="ACZPCL_10305"
     ncRNA           5031..5384
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACZPCL_10305"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            5522..6046
                     /locus_tag="ACZPCL_10310"
     CDS             5522..6046
                     /locus_tag="ACZPCL_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007886715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036094 - small molecule binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3H domain-containing protein"
                     /protein_id="HIR1162896.1"
                     /translation="MTEGGKRRDGILSLLEESDTPLSGTELAHRFKVSRQVIVQDIAL
                     LRAEDKKILSTYRGYVLDRESEKKRKCIRVFCVCHSTKDTQDELQTIVDYGGHVLDVA
                     VDHPLYGQIRADLIINNRLDVAEFVDRMNRYNAQPLKVLTGDYHYHTVTAESEKNLDF
                     IEQELRKKGYLKEE"
     gene            complement(6207..>7023)
                     /locus_tag="ACZPCL_10315"
     CDS             complement(6207..>7023)
                     /locus_tag="ACZPCL_10315"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HIR1162897.1"
                     /translation="DQLSKVSGVEVKDFASLKEALKNRMDFFTSMKCCVSDHALEYVM
                     YVPATEDEVNAIIAKGLKGEAISREEELKYKTEFMLFVAKEYTKRNWVMQLHYGCKRD
                     NNAYMFDKLGADTGYDCINNYAPSAQMADFLNALSATNDIPKTIIYSLNPNDNASIGT
                     IIGCFQEKFPGKIQQGSAWWFNDHKVGMTDQMVSLANLGCLGNFIGMLTDSRSFLSYT
                     RHEYFRRVLCALVGGWVENGEYPADMKSLESIVKGISYNNAVQYFGFDLDVVK"
BASE COUNT         2111 a         1344 c         1865 g         1703 t
ORIGIN      
        1 tccggaagaa atgttatttc cagggaaaat ggaagcggtc caggtaatga tatgggccgg
       61 ccggaatatg gcggcaatcc ttatgaggga caggcagcgc agaaccaggc ggccataaag
      121 aaaacaggaa gaaagcagga gcgggaagac ggcatacgaa gatcccagag actgctgctt
      181 acctggctga tcgaaaatcc ggcgttattt gacaagataa aaggcattat aacagccgat
      241 gattttgtgg aagacctgta ccatcaggtg gcagtcatgg tatttgaagg ccatgaagca
      301 ggaaatgtaa atccggcagg tattttaagc cggttcatca atgatgaaga ccagtacaaa
      361 gaagtagcgg cattatttaa tgccagctta aaagaatcct taaacaatga ggagcagaaa
      421 aaggcatttg cggaaactgt gatgaaagtg agaaagaaca gtttggatac cgcaagccgg
      481 aatgcgaaag atatagccca gctgcaggag atcataaagc agcaggcagc gttaaaacag
      541 ctgcatatat ctttggattg atttaaggag gaacactatg gaaagaacgc gcagagagga
      601 atctttaagc tttaaggaaa aaatgggacg gcttttagca gatgcccgtg ccgggaaacg
      661 gcagttggaa tatcgtgata ttttccggta tttcagacca aaagagctga aagaggaaga
      721 actggaccgc gttcttgaca tattagcgga aaaccaggtt cagatcctgg atgaagggga
      781 agagcaggat gcaggggaaa gtgaacctgt atcagaggaa aaaacggggc aggcggatcc
      841 ggtctgcctg tatttaaaag aaattggaaa aatttccctt ttaaccccgg aagaagaatt
      901 aatgctggca aaggaagtaa gggccggaag cagagaagca aaagagaaac tggcaagggc
      961 aaatctgcgg ctggtagtca gtattgccaa gcattacgca ggcaggggaa tggcattttt
     1021 agatctggtc caggaaggaa acctggggct tatgcgggcg gtagagaagt ttaattacga
     1081 aaaaggctac cgtttcagca cctatgctac ctggtggatc cgccagtcca ttacaagggc
     1141 gatagcagat caggcaagga ctatccgcat cccggtgcat atggtagaag tcatcaaccg
     1201 ggtgatgcga agttccagaa ggatgcttca ggaactggga cgggaaccta ctactcagga
     1261 aattgcagaa cgcttacata tgaccagcaa aaaagtagaa gaagtgctga atatggccca
     1321 ggagccggtt tctttggaaa caccggtggg agaggaagag gacagtcatc tggtggattt
     1381 tatccaggat gaaaaaatgt cttttttgca ggatgaggct tcttttgtgt ttttacatga
     1441 gcagctgatg gaagtattaa aaacgttgac tccgagagaa cagcaggtat tgtcattgcg
     1501 gtttgggctt tatgacgggc agccaagaac attggaagaa gtgggaaagc agtttcatgt
     1561 gaccagggaa cggatccgcc agatcgagga taaagccctg cgcaaactgc gccaccccag
     1621 cagaagccgg aaattaaagg actatttaga aagttgaagg atagatatga cagaaaagat
     1681 acaggaaaca gagataaaga agacaaggct tcgcctttca gaacgtttaa agctggttgc
     1741 ttcttttgtg ccggaaggca gccggatcgc agatatcggt acagatcatg gatatgtgcc
     1801 tatttatctg gcagaaattg gaaaaattaa aagtgctctg gctatggatg tgcgaaaagg
     1861 tcctttggca agggcagatg aacacataga agaatacaga catgacgtgg gggatgcagc
     1921 tatttccata gagacccgtt taagcaacgg actggaaaaa ttacaggcca aagaagccga
     1981 tacagttatc attgcgggaa tgggcggtga actggagatc agcattttag aggctggaaa
     2041 gcagttatgg agcagtatcc gccactggat cttttcaccg cagtcggatt tggaaaagtt
     2101 ccgccgttat ctgaaggaaa atggcttctt tattgaaaac gaggcaatga tactggatga
     2161 gggaaagttt tatacagtga taaaagctgg ttttggaaca aatgaaaaag acgcttgtgt
     2221 ctgtaaaacg ctccctgagt atcgcttcgg agcggttttg atcaaacgca gggatgccgt
     2281 tttaaagcag tatcttttaa aagaagaaaa aagagtggaa gggatcttaa aaggatttgc
     2341 aggaaagagc gaggaagagc tgacggcagg acaggcagct gcctgcaagg ctttaaaaga
     2401 agaactttca tgtatcaggg aggcacaaga tgaaatgcag tgaagtgatc gaaatattgg
     2461 gaaaactggc tcctgaaagc tgtgcctgtg attgggataa tccggggctg ttagcaggac
     2521 gcagtgataa agaggtaaaa aaaatctata ttgctttaga tgcaacggac caggtggtgg
     2581 atgcagccat taaggcagga gcagatatgc ttttgaccca tcatcccctt atttttaagg
     2641 ctattaaaaa agtgaatgac cagaatttta ttaccagacg tctggtaaag ctgatccagg
     2701 cagatatttc ctactatgcc atgcacacca attttgatgc agcacccggc tgtatggcag
     2761 atctggcagc agaacgtctt tctttaaagg atacggagcc gttggaagct atgggggata
     2821 tggaaataaa tggaaattct gtggcatatg gtattggaaa aacaggaaac ctggcggaaa
     2881 agctgactgt aaaagcactg gcagaaaaag taaagcaggc ttttgatctt ccttttgttc
     2941 tggtgtatgg ggaagaactg atggatatgg aagtagaaaa agtggcactt tgtcctggag
     3001 ctggcggaag tgtgatcgaa gaagccatcc atgcaggcgc aaaggcgctg gtaacaggtg
     3061 atatcagcca tcaccaggga attgatgcag cagccaggaa tatggcagtg atcgatgcag
     3121 gtcattacgg aatggagcat atttttatcc agtatatggc tggatatctg aaggaaaatc
     3181 tgccggtaga gatggagatc gttacagcgc catctgcatg gcctacagtt cttttataat
     3241 tgttttctat gtacagcaat acatttttga aaaggaggat gcctatggtc caactgaaaa
     3301 taaataccca gataaaggaa tatccggatg atattacctg gcaggtgata gcagatgagt
     3361 atcaaagcgg gtattccgat gaaatattat tagtccaggt aaatggaaaa ctccaggaac
     3421 tgcaggaaaa gataagggaa ggcgaagtgc agttcattac tgcaagacaa aagccgggaa
     3481 tttccgcata tcagcgaagt gccacccttc tgatgctgaa ggcattttac gcagtggcag
     3541 gtcctgaaaa tgtggaaaag gtcattgtgg atttttctat tggaaaaggt ttttttgtag
     3601 aagcaagagg aagtttcaca ttgaaccagg agcttcttga ccaggtgaaa gccaaaatgc
     3661 aggaatacgt agatcaggag atcccgattt taaagcgcag tatttctacg gatgatgcca
     3721 tagagcgttt tcacaaacac cggatgtacg ataaggagcg tctgttcctc taccgccgtg
     3781 tatccagagt caatgtttac agtattgacg gatttgagga ttatttctac ggatatatgg
     3841 ttcccaatac aaaatatatc cgttatttca atctgaagtt gtatgaatat ggctttgtgc
     3901 tcatgctgcc gtctatgttg gcaccaacag tgctgccgga gtttgcgccg ctgccaaagc
     3961 tgttccatac acttgcggac tcttcagagt gggggaaacg tctggatctg gagacagtag
     4021 gcgcattaaa tgacaagatc gcagctggag atatgtccca tttgatcctg atccaggagg
     4081 cgcttcagga gaaacggatc gcagagatcg cggcccagat cgcagagcgc aaaactgcca
     4141 agatcgttat gatcgcaggt ccttcttcct ctggaaagac aacattttcc catcggttgt
     4201 ccgtacagtt ggaggctatt ggcttaaagc cgcatccaat cgcggtagat aactattttg
     4261 tcaaccgtgt ggacagtcca agggatgaaa atggtaatta taattatgag atcctggaat
     4321 gtctggatgt agaacagttt aatcaggata tgcaggctct tttaagagga gagcgtgtgg
     4381 aactgcctta ttataacttc aaaaaaggag aacgggaata caagggagat tttctgaaac
     4441 tgggaaaaga tgatatcctg gtgatcgagg ggatccattg cttaaatgac cgtttatcct
     4501 attctcttcc aagagagagc aagtttaaga tctatatcag tgctctgacc cagttaaata
     4561 ttgacgagca caaccgtatc cctacgacag atggccgtct gctgcgccgt atgatccgtg
     4621 atgcaaggac aagaggggcg tcagcccagg ataccatccg tatgtggcca tctgtgcgaa
     4681 agggagaaga ggaaaatatt ttcccctatc aggaagaggc agatgctatg tttaactctg
     4741 ctctcattta tgagctggca gtgttaaaac agtatgtgga gccgctgctt tttggaattc
     4801 caaaggattg tccggaatat acagaagcaa agcgacttct taaattcctg gattatttta
     4861 tcggggtcag cagcgaggat ataccgaaaa actctatttt aagggaattt atcggcggaa
     4921 gctgtctgga tgtgtaggat agcaagtgtc cacatatgat atttttaata tgatattttt
     4981 ggatttaccc atggacatgg caaagaaact atgctaaaat aaacaagttg tgagtaagac
     5041 agatggtcgc tgctgcgaca ccgagaggac gcaggagagg aaagtccggg cttcacaggg
     5101 caggatgcca gataacgtct ggcgggggtg accctaggga cagtgcaaca gaaatatacc
     5161 gcccgctgca tttgcagcag gtaagggtgg aatggcagtg taagagacta ccgcccggct
     5221 ggtaacaggc ggggcacatg taaaccccat ccgaagcaag accaaacaga gagcatatgg
     5281 cggcccgtca gctttcgggt aggttgcttg atgctgctgg cgacagtagt actagataga
     5341 tgaccatcca acgacataac ccggcttacc gttttactca caacctttgc aacgatgaag
     5401 tgcgtctcct tgaatgaaag gggacgcttt ttagcataaa agaaaacgta tgcagtatac
     5461 agcgaatggt cagactgctc tcaatgagag aacatgctgc gcaaaaggag ggaattttat
     5521 catgacagaa ggcggaaaaa gaagagatgg cattctttca ctgttagagg agagcgacac
     5581 tccattatct ggaacagaac tggctcatcg ttttaaggta agccgccagg tgatcgttca
     5641 ggatattgct cttttgcggg cagaagataa gaagatcctt tctacttaca ggggatatgt
     5701 tcttgaccgg gaaagtgaaa aaaagagaaa atgtatccgg gtgttctgcg tctgccattc
     5761 taccaaagat acccaggacg aacttcagac cattgtagat tatggtggcc atgtgctgga
     5821 tgtggcagtg gatcatccgc tgtatgggca gatccgtgca gatctgatca tcaacaaccg
     5881 cctggatgta gcagagtttg tagaccgcat gaaccgctac aatgcacagc ctttaaaggt
     5941 tttgaccggg gattaccatt atcatacagt gactgcggaa tcggagaaaa acctggattt
     6001 tattgagcag gaactgcgga aaaagggata cttaaaagaa gaatagagat aagaaaagta
     6061 attgcaggca ttaagaaagc gcccaacctc acattgtgag gcggggcgct ttttgcatct
     6121 ggtgtagact cacgattaga ctcacgaatt ccgtgctgac gcactctact gtctgcttgc
     6181 gcagacaatt cgttcgttgt ccctatttat ttcacaacat ccagatcaaa tccgaagtac
     6241 tgaacagcgt tgttgtagct gatgccctta acgatggact ctaaggactt catatctgcc
     6301 ggatattcgc cgttttctac ccagccgcca accagtgcgc agagaacgcg acggaagtac
     6361 tcatgtctgg tgtaggataa gaagcttctg gagtcggtca gcataccgat gaagttgccc
     6421 aggcagccta agttagccag ggataccatc tgatcggtca tacctacctt gtgatcattg
     6481 aaccaccaag cggaaccctg ctggatcttt cctgggaatt tctcctggaa gcagccgatg
     6541 atggtgccga tggaagcgtt gtcgtttgga tttaaggagt aaatgatagt cttaggaata
     6601 tcattggttg cgcttaatgc atttaagaaa tctgccatct gtgcgcttgg agcgtagttg
     6661 ttgatgcagt cgtaaccggt atctgcgcct aacttgtcaa acatgtaagc gttgttgtca
     6721 cgcttgcatc cgtagtgaag ctgcataacc cagtttctct tggtgtattc ttttgcaacg
     6781 aagagcatga actcggtctt gtactttaat tcttcttctc tggagattgc ttcgcccttt
     6841 aagcccttag cgatgatagc gtttacttca tcctcagtag ctggaacgta cataacatac
     6901 tctaatgcgt ggtcagatac acagcacttc atggaagtaa agaaatccat acggttcttt
     6961 aaagcttcct ttaaggatgc gaaatctttt acttcaacac cgctgacctt ggataactga
     7021 tcc
//
LOCUS       DBMDUA010000148         6995 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4577_length_6995_cov_2.595155, whole genome shotgun sequence.
ACCESSION   DBMDUA010000148 DBMDUA010000000
VERSION     DBMDUA010000148.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4577_length_6995_cov_2.595155"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            29..1555
                     /gene="galT"
                     /locus_tag="ACZPCL_10320"
     CDS             29..1555
                     /gene="galT"
                     /locus_tag="ACZPCL_10320"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIR1162898.1"
                     /translation="MVYEAIRELVRYGLEKGLITEDDAIYARNQILEVLKMDEYEEPE
                     KDGELTGADLEKILKVLLDYAAEKGLLQENSVVYRDLFDTKLMNCLMPRPSEVTKTFW
                     EKYQVSPETATDYYYNLSRNSDYIRRYRVSKDMKWTTDTKYGTLDITVNLSKPEKDPK
                     AIAAAKLAKQSGYPKCQLCMENIGYAGRVNHPARNNHRVIPITINDSKWGFQYSPYVY
                     YNEHCIVFNGQHIPMKIERATFRKLFDFISQFPHYFLGSNADLPIVGGSILSHDHFQG
                     GHYTFAMAKAPIEKYYKAEGYEDVEAGIVYWPMSVLRLRSKSCDSLIDLGDKVLKAWR
                     GYTDEAAFVYAETEGEPHNTITPIARKNGDMYELDLVLRNNITTEEFPLGVYHPHQEL
                     HHIKKENIGLIEVMGLAVLPSRLKKELAELAEYIVEGKDIRSNPDIEKHADWVDSFLP
                     VYKEKGIEITKDNAEDILKEEVGQVFAKVLEDAGVYKCTTEGREAFERFLNTVDFHKE
                     "
     gene            complement(1654..3075)
                     /locus_tag="ACZPCL_10325"
     CDS             complement(1654..3075)
                     /locus_tag="ACZPCL_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HIR1162899.1"
                     /translation="MYFTDQLEENMTWLNQALHVDKNFDVVYRVLTIADKKACLYFID
                     GFTKDDSLLKILQGFSSIKTDDMPEDAHSFSKKYLPYGEIGLLSDSREMIIQLLSGVS
                     CLFIDGYDKCLTIDCRTYPARGVSEPEKDKVMRGSRDGFVETLVFNTALIRRRIRDPK
                     LTMEILTTGESSHTDIAMCYMEGRADKKLIETIKKRIQNVKVDALSMNQESLAECIFP
                     GKWFNPFPKFKFSERPDTSAASILEGNIVILVDNSPSAMILPSSVFDIIEEADDYYFP
                     PVTGTYLRLSRMLISLLSMLLTPTWLMLMQNTELIPHWLRFIRLSDPCNIPLIWQLLI
                     LEFAIDGLRLAAVNTPNMLTTPLSVIAGIVLGEYAVESGWFNSETMLYMAFVTIANYS
                     QASFELGYAMKFMRIIILICTAIFNIWGFVIGIIFSFCAIIFNRTIAGKSYIYPLIPF
                     HLNELKKRFLRGRLPHKLEEDEN"
     gene            3565..4473
                     /locus_tag="ACZPCL_10330"
     CDS             3565..4473
                     /locus_tag="ACZPCL_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1162900.1"
                     /translation="MGKTSSLKTILEQIKLLQKDKKIRLVITMAAVLLSAFIQAWAIQ
                     VFVRPAEIISGGFSGAAMLIERVGSLKGLTIPVQVTMILLNIPVALMCCRGISVRFTV
                     VSLLQVVFGSIFLQIFSFQPIFTDEILNVIFGGVIYGTSIVIALRGNASTGGTDFIAL
                     YVSNKTGKSIWSYVFFGNALMYCVYGVIFGWKFAGYSIIFQFISTHMISAFHHRYDLV
                     TLQATTEKGPEVVALYIKHFRHGISCVEAVGGYSGRKMYLLNTVISSYEIADAVHVMQ
                     EADEHVIINVLKTEQFVGHFYRAPME"
     gene            4849..6819
                     /locus_tag="ACZPCL_10335"
     CDS             4849..6819
                     /locus_tag="ACZPCL_10335"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIR1162901.1"
                     /translation="MKDGFIRVAAATPDIHVADPQYNSEQIIELMEQGSARGVKVMAF
                     PELCLTAYTCSDLFLQESLLREAKKELKRILQASKSKDMILFVGMPWMHKGKLYNVAA
                     VLNRGRILGIVPKKHLPNYSEFYELRHFNPGMEKVEMTDWEGVQVPMGMNLLFACRTM
                     PELVLAAELCEDVWVPCPPSISHALAGATVIANCSASDETTGKDQYRYNLICGQSARL
                     VCGYIYANAGEGESSQDLVFGGQNIIAENGTCLVQSDRFKNACICADLDLGRIISERR
                     RMTTFRDEENRSASYETVYFELNTEDSAKKDSKKAGQKRDELLRYIDPAPFVPHDAGQ
                     RERRCEEILSIQAMGLKKRLAHTNCKHAVIGLSGGLDSTLALLVTVRAFDMLQIPREN
                     IHCITMPCFGTTDRTYTNACTMAKKTGASLTEINIRDAVTGHFKDINHDIEKHDVTYE
                     NGQARERTQILMDIANEVGGMVIGTGDMSELALGWATYNGDHMSMYGVNASVPKTLVR
                     HLVRYYADTCGEKELSDVLLDVLDTPVSPELLPPEDGKISQKTEDLVGPYELHDFYLY
                     YVLRFGYSPAKIFRLALQAFEGSYDRETILKWLKTFYRRFFAQQFKRSCLPDGPKVGS
                     VAVSPRGDLRMPSDACSRLWLKELEMIEKGER"
     gene            6821..6967
                     /locus_tag="ACZPCL_10340"
                     /pseudo
     CDS             6821..6967
                     /locus_tag="ACZPCL_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587602.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
BASE COUNT         2089 a         1385 c         1753 g         1768 t
ORIGIN      
        1 atttgcaaat aaaaaggggg atatgcagat ggtttacgaa gcaatcagag aattggtacg
       61 ttacgggtta gagaagggcc tgatcacaga agatgatgcc atttatgcca gaaatcagat
      121 cctggaagta ttaaaaatgg atgaatacga agagccggag aaagatgggg aacttacagg
      181 cgcagatctg gagaagatcc tgaaggtact tcttgactat gcagccgaaa aaggactttt
      241 acaggaaaac agtgttgtat acagggatct gtttgataca aaactgatga actgcctgat
      301 gccaaggcca agtgaagtaa caaaaacatt ttgggagaaa tatcaggttt cgccggagac
      361 tgccaccgat tactattaca acctgagccg gaattccgac tatatccgca gataccgtgt
      421 aagcaaggat atgaaatgga ccacagatac caaatacggt acactggata tcacagtcaa
      481 cttatccaaa ccggaaaaag atccaaaggc tattgcagca gcgaaactgg caaagcagag
      541 cggttatcca aagtgtcagc tttgtatgga aaatatcggt tatgcaggca gagtgaacca
      601 tccggcaaga aataaccatc gtgtcatacc gatcaccatt aatgacagca agtggggatt
      661 tcagtattca ccttatgttt actataatga gcactgtatc gtgttcaatg gccagcatat
      721 cccaatgaag atcgagcggg ctacattcag aaagctgttt gattttatca gccagtttcc
      781 gcattatttc ctgggatcca atgcagatct tcctattgta ggcggatcta ttttaagcca
      841 tgaccatttc cagggcggcc attatacctt tgcaatggca aaagcaccca tcgagaagta
      901 ttataaggca gaaggatacg aggatgtaga agcaggtatt gtttactggc caatgtccgt
      961 actgcgtctg cgttctaagt cctgcgactc cctgatcgat ctgggggata aggtgttaaa
     1021 agcatggaga ggctatacgg atgaggcagc tttcgtgtat gcagaaacag agggagaacc
     1081 tcataacacc attaccccga tcgcaagaaa aaacggggat atgtatgaac tggatctggt
     1141 gctgcgaaat aacattacta cagaagagtt cccactgggc gtttaccatc cacatcagga
     1201 actgcaccac attaagaaag aaaatatcgg tctgatcgaa gtaatgggac tggcagtgct
     1261 gccatccaga ctgaaaaaag aactggccga actggcggag tacatagtag aaggcaaaga
     1321 tatccgcagc aatccggata ttgaaaaaca tgcggactgg gtggacagct tcctgcctgt
     1381 atacaaagaa aaaggcattg agatcacaaa agacaatgcg gaagatatct taaaagaaga
     1441 agtgggtcag gtatttgcca aagttctgga agatgccggt gtttataagt gtacaaccga
     1501 aggaagagag gcttttgagc gtttcctgaa tacagttgat tttcataaag agtagagata
     1561 aagggtcccg cctggtggcg ggacctgttg ttttgagaaa taccggtgat acgtccggag
     1621 attgcctttg ggtgcagtgc tgttttttgg tggttagttt tcatcctctt ccagcttgtg
     1681 aggcagccgc ccgcgcagga accgcttctt taactcattt aagtgaaatg ggatcagagg
     1741 gtaaatataa ctttttcctg caatggtcct gttaaagata atggcgcaga aagagaagat
     1801 aataccgatc acaaagcccc agatattgaa aatggcagtg cagatcagta tgatgatgcg
     1861 cataaatttc atggcgtagc caagttcaaa gctggcctgg gaatagttgg caatggtgac
     1921 aaaggccata tacagcatgg tttcggagtt gaaccacccg gactctaccg catattctcc
     1981 cagcacgata cctgcgataa cgctgagtgg cgtagtaagc atgttgggag tgtttaccgc
     2041 tgccaggcgc agaccgtcaa tggcaaattc aaggatgaga agctgccaga tgagaggaat
     2101 attacagggg tcagacagcc gtataaaccg gagccagtgg gggatcagct ccgtattttg
     2161 catcagcatt agccaggtag gtgtcaggag catggagagc agggagatca gcatgcggct
     2221 taaacgcaga taggtacctg taaccggcgg aaagtaataa tcgtctgctt cttcaataat
     2281 atcgaataca gaagagggca ggatcatggc agaaggagaa ttgtccacca ggatcacgat
     2341 atttccttct aaaatagagg ctgcagaggt atccggccgt tccgaaaact taaatttggg
     2401 aaacggatta aaccattttc ccggaaaaat acattctgcc agactttcct ggttcatgga
     2461 aagggcgtct acttttacat tctgtatcct ctttttgatc gtctcgatca gttttttatc
     2521 tgcccggcct tccatataac acatggcaat atctgtgtgg gagctttctc ctgtagtaag
     2581 gatctccatg gtcagcttgg ggtcccggat cctgcgacgg atgagagctg tattaaatac
     2641 cagcgtttct acaaaaccat ctctggaacc acgcatgacc ttgtcttttt ccggttcact
     2701 gactcctctg gcgggataag tacggcagtc gatggtaaga cacttgtcat agccgtcaat
     2761 aaaaagacag gaaacaccgg aaagcagctg tatgatcatt tcacggctgt cagataaaag
     2821 accgatctca ccatagggca gatatttttt tgaaaagctg tgggcatctt ccggcatatc
     2881 gtcagtcttg atggaagaaa agccctgtaa tatttttaag agagagtcat cttttgtaaa
     2941 accgtcaata aagtataagc aggctttttt atctgcaata gtaaggacgc ggtagacaac
     3001 gtcaaaattt ttatccacat gaagggcctg gttcagccag gtcatatttt cttccagctg
     3061 gtccgtaaaa tacatggaaa actcctttct gctgacaaaa ccacaaaagg acaaatgtct
     3121 gccatacaaa gacaaaatgt gaataacttg gcaattatgt gaaataacac acaaacattt
     3181 ccgtaattgt cagacttgta gagtagtata tccggaagtc agaaaactat gtaaggtctt
     3241 taagggatat gttacatctg ccagtgggga acaaaacaaa aatcgacaaa aaccgacgaa
     3301 ttttatagga taatacctaa aaaaataaaa ggtatttgct aaaatgcaca aaaagattga
     3361 caaaactgtg aaacgatgct atagtaacat tcgtaagcag cacaaaggag tgccttacag
     3421 gtttttgttt ggtatgggga tgccagcaat attttatagt gtgacggttt ttgatcgtga
     3481 ctaagagctg ccttctgtgg gggaagggca gctcttcttg tatctgtgag aaatacaaac
     3541 agagtggcgg gaggacggga taatatggga aaaacaagca gtttgaaaac gatacttgaa
     3601 cagataaaac ttctgcaaaa agataagaaa atacgcctgg tgatcactat ggctgcagtg
     3661 cttctctctg cttttatcca ggcctgggcc atccaggtgt ttgtaagacc ggcagagatc
     3721 atttccggtg gtttttccgg tgcggccatg ctgatcgaac gtgtgggaag cttaaaagga
     3781 ctgactattc ccgtgcaggt gacaatgatc cttttaaata tcccggtagc tcttatgtgc
     3841 tgtcgtggga tcagtgtccg gtttacagtg gtgtcactgc ttcaggtcgt atttggaagt
     3901 atatttttgc agatattttc atttcagcct atttttacag atgagatctt aaatgtgatc
     3961 ttcgggggcg tgatctatgg tacatccatt gtgatcgcac tgaggggcaa tgcatctacc
     4021 ggtggtacgg actttatcgc gctgtatgta tccaataaaa caggaaagtc catctggagt
     4081 tatgtattct ttggaaatgc cttaatgtac tgtgtatacg gtgtgatctt tggatggaaa
     4141 tttgcgggat attccattat tttccagttt atatccacgc atatgatctc agctttccac
     4201 caccgctacg atctggtgac acttcaggca acaacagaaa agggaccgga agtggtagcg
     4261 ctttatatca aacattttcg ccatggtatt tcctgtgtgg aagcagtagg gggatacagc
     4321 ggaagaaaga tgtatctttt aaatacggtg atctcctctt atgaaatagc agatgccgtt
     4381 catgtgatgc aggaggcaga tgagcatgtg atcatcaatg tgttgaaaac agaacagttt
     4441 gttggacatt tttacagggc gcctatggag taggcgtcct tttttggacc caaaataact
     4501 gcaaaagcag gtgaaacagg cgtttaaggc aaaaaaataa agccctggca agtgtggtag
     4561 tagtggtgca gggctttata aaatgtacag aacaggcgtt ctgaacactc tgagtataca
     4621 gcagattgaa caaatagtca agaagaattt gtgctggcag cgccagcgac tatgtttttg
     4681 tggagaattg tgctataatg agcgcaaaag aaaagcaagc gctgcacaat gcagcatttg
     4741 cttttcttgc gccattaacg aacggatcgc ctgcggcagc aggcagtaga gcacgtcagt
     4801 gcgggatccg tgagtgaccc ggtaacgaag gaatacacgg aggacacaat gaaagacgga
     4861 tttataaggg tagcagcggc aacgccagac atccatgtgg cagacccgca gtataacagc
     4921 gaacagatca tagaattaat ggaacaggga agcgccagag gtgtaaaggt tatggcgttt
     4981 ccggagctgt gtttaacagc gtatacctgt tctgatttat ttttacagga gtctctgtta
     5041 agagaagcta aaaaagaatt aaagcgcatt ttacaggcat ctaaaagcaa ggacatgatc
     5101 ctttttgtag gaatgccctg gatgcataaa ggaaaacttt acaatgtggc agctgtgtta
     5161 aacagaggcc gtatcttagg tatcgttcct aaaaaacatc tgccaaatta ttctgaattt
     5221 tacgaactgc gccattttaa tccgggaatg gaaaaagtgg agatgacaga ctgggaaggc
     5281 gttcaggtac ctatgggcat gaacctgctg tttgcctgcc gcaccatgcc ggaactagtg
     5341 ctggcagcag aactttgcga ggatgtatgg gtaccatgcc cgccaagtat ttcccatgct
     5401 ttggcaggtg caacagtgat cgccaactgc tctgccagtg atgaaacgac aggaaaggac
     5461 cagtatcgtt ataacctgat ctgcggacag tctgcaaggc tggtgtgcgg ttatatttat
     5521 gcaaatgcag gagaagggga gtcatcccag gatctggtat ttggcggaca gaatatcatt
     5581 gcggaaaacg gcacctgtct ggtacagtct gaccggttta aaaatgcctg catctgtgca
     5641 gacctggatc ttggaaggat catcagcgag cgccgccgta tgactacttt cagggatgag
     5701 gaaaacagat ccgcatccta cgaaaccgta tattttgaat taaatacaga agacagtgca
     5761 aagaaagaca gtaaaaaagc aggacagaag agggacgaac ttctccgtta tattgatccg
     5821 gctccatttg tgcctcatga tgcaggccag agagagcgcc gctgcgaaga gatcctttcg
     5881 atccaggcaa tgggactgaa aaagcgtctg gcacatacaa actgcaaaca tgcagtgatc
     5941 ggtctttccg gcggtctgga ctccacactg gcacttcttg taacagtacg tgcttttgat
     6001 atgctgcaga tcccaagaga gaatatccac tgcatcacta tgccatgttt cggaacaaca
     6061 gaccgtactt ataccaatgc atgtaccatg gcaaagaaaa caggagcatc tttaactgag
     6121 atcaatatcc gtgatgcggt taccggccat tttaaggata ttaaccatga tattgaaaag
     6181 cacgacgtaa cttacgaaaa cggacaggca agggagcgta cccagatctt aatggatatt
     6241 gcaaacgagg taggcggtat ggtcatcggt accggtgata tgtctgagct ggctcttggc
     6301 tgggcaacct acaacggcga tcatatgtcc atgtacggtg tcaatgcatc tgtgccgaaa
     6361 acattagtcc gccatctggt acgttattat gcagatacct gcggcgaaaa ggaactttcc
     6421 gatgtgcttt tagatgtcct tgatacaccg gtaagcccgg aacttctgcc tccggaagat
     6481 ggaaaaattt cccagaaaac agaggacctg gtaggtcctt acgagttaca tgatttctat
     6541 ctgtattatg tactccgttt tggctacagc cctgcaaaga tcttccgtct ggcgcttcag
     6601 gcatttgagg gaagttatga cagagagacg attttaaagt ggctgaaaac attttaccgc
     6661 agattttttg cacagcagtt taagcgttcc tgtctgccgg atggcccaaa ggtaggttct
     6721 gtagctgttt ccccaagagg tgatctgcgt atgccaagtg atgcctgcag ccgtctgtgg
     6781 ttaaaagagc tggagatgat agaaaaagga gaaagataat atgataaaca gtacctccaa
     6841 taagcaggta aaacgggtca tcaacctgag ggataaggcc aaagcccgca gggaagaagg
     6901 cttatatgta gcggaaggac tgcgcatgtg ccgggaatat aagccggatg atgtggagtg
     6961 tattttttgt tttaaacctt taaattgatt tatta
//
LOCUS       DBMDUA010000149         6989 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4581_length_6989_cov_2.505662, whole genome shotgun sequence.
ACCESSION   DBMDUA010000149 DBMDUA010000000
VERSION     DBMDUA010000149.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6989)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4581_length_6989_cov_2.505662"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            328..1653
                     /gene="rsxC"
                     /locus_tag="ACZPCL_10345"
     CDS             328..1653
                     /gene="rsxC"
                     /locus_tag="ACZPCL_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HIR1162902.1"
                     /translation="MGLATFKGGIHPYEGKELSENKPIQVLLPKGELVFPMSQHIGAP
                     AKPLVAKGDRVLAGQKIGEAGGFISANVICSVSGTVKAIEPRRMVNGAMVPSIIVEND
                     GEYETVEGVGEDRDPSTLSKEEIRSIVKEAGIVGLGGAGFPTHVKLTPKDENAIDYVI
                     VNGAECEPYLTSDYRMLIEEPEKIVGGLKVILQLFDNAKGVIAIENNKPEAIKKLTEM
                     VKDEPKITVCPLLTKYPQGGERSIIYAVTGRKVNSSMLPADAGCIVDNVDTVASIYMA
                     VCKSTPLMRKIITITGDAVAEPQNFNVKLGTNYQELIEAAGGFKTEPEKIISGGPMMG
                     QALFTVDVPVCKTSSALTCFTKDQVAAFEPTACIRCGRCVSVCPSHIVPVMMMKAAMN
                     RDCETFEKINGMECMECGSCTYICPARRPLTQAFKEMRKTVAANRRKKG"
     gene            1669..2610
                     /locus_tag="ACZPCL_10350"
     CDS             1669..2610
                     /locus_tag="ACZPCL_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009218334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR1162903.1"
                     /translation="MNKLLNVSSSPHVRCSVNTQNLMLDVVIAMLPAAAFGVYNFGFH
                     ALLVLLVTSAVCVASEYVYEKLMHKPITIADGSALVTGMILALNMPPQIPVWVPALGG
                     IFAIIVVKQLYGGLGQNFMNPALAARCFLLISFAGMMNNFSAASLGFDSVSGATPLAA
                     MRAGQSVDLASMFIGRIPGTIGEVSTIALLIGGIYLLVRKVISWRIPVIYIGTVAVFV
                     FIFGGFDVNYVLCQVCGGGLIFGAFFMATDYVTSPITPMGQIVFGVVLGLLTGIFRLW
                     GASPEGVSYAIILSNLFVPMIERVTLPKAFGKEAKKA"
     gene            2607..3215
                     /locus_tag="ACZPCL_10355"
     CDS             2607..3215
                     /locus_tag="ACZPCL_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HIR1162904.1"
                     /translation="MKGILKDAAILFVITLVAGLCLGAVHEVTLDPIAKAQEAAATKT
                     YKEVFPEAAKFEQTDELTAAVAASADEILAQGFGNVSVDDAQVAEAEDGSVLGYLITA
                     SSTEGYNGLVQISVGITSEGNLTGLGFLSISETPGLGMKAKEPDFKDQFNGMKAQKLE
                     VTKTDATADNQVQAISGATYTSKAVTGATNAAIYFVENCVGK"
     gene            3228..3908
                     /locus_tag="ACZPCL_10360"
     CDS             3228..3908
                     /locus_tag="ACZPCL_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009536615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit E"
                     /protein_id="HIR1162905.1"
                     /translation="MNKYTERLYNGIWKENPIFVQMLGLCPTLAVTTSAMNGLGMGLS
                     STVVLVAANFIISLMRSIIPGRVRLPGEIVVVASLVTIVDMLMEGFTPDLYSALGIYI
                     PLIVVNCVILGRAEAFAMKNKPIISAFDGAGMGLGFTFALVCIGAVRELLGAGSIFGV
                     AIMPASYQPISIFILAPGAFFVLACLTALQNKLKMPSATNGSAKGKKAKTACCGDCAN
                     CAESKTEE"
     gene            3922..4497
                     /locus_tag="ACZPCL_10365"
     CDS             3922..4497
                     /locus_tag="ACZPCL_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex protein RnfA"
                     /protein_id="HIR1162906.1"
                     /translation="MKELLVIAISSAFVSNVVLSQFLGICSFVGVSKKVETAAGMGGA
                     VIFVITIASVVASLIYQFVLNPLGLDYLKTIVFILVIAALVQIVEMFLKKCMVSLYNA
                     LGVFLPLITTNCAVLGVALTNVQNGYSFVQSVVNGVATAVGYTVSIVLLAGIRERIEG
                     NDIPYNFQGSPIVLVTAGLMAIAFFGFSGLL"
     gene            4513..5304
                     /locus_tag="ACZPCL_10370"
     CDS             4513..5304
                     /locus_tag="ACZPCL_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HIR1162907.1"
                     /translation="MNITAILIAAAVIAVAGIIVGIGLGLFGEKFKVEVDEKEVAIRE
                     ELPGNNCGGCGFAGCDALAKAIAEGKAPANACPVGGAPVGEKIAAILGVSAGAAEKKV
                     AFVRCKGTCDKVKTLYNYEGIDDCAKASVVPAGGPKACSYGCTGFGSCVKACQFDAIH
                     VVDGVAVVDKEKCVACGKCVATCPKGLIQIVPYSAEHLVQCSSHDKGKDVKAACQAGC
                     IGCTLCTKQCESDAIHMDNNLAFIDYEKCTNCGKCAAKCPAKVIL"
     gene            5940..6782
                     /locus_tag="ACZPCL_10375"
     CDS             5940..6782
                     /locus_tag="ACZPCL_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HIR1162908.1"
                     /translation="MLTWHSFLQSVMFFVRSISVKVTKNHYRSSSVLMSGAVVIAVVA
                     FSANGFGGGGKNAMAAPRTVDSLEADEEPETEALFEGSSFVTEAKIQFGLLNTGSEGQ
                     HLAGALLERDVRDRQRRQADAQIEIEALQKQILKEKEEAEARRKAEEERRAARRIKVS
                     DEDYQVLLRIVQAEAGVCDEKGKILVADVIINRVLSGKFPGSVKGVVYQGSQFQPVSN
                     GRINSVKVTAETIRCVDRALDGEDYSNGALYFMNRKASGRKTGWFDSRLTYLFAHGGH
                     EFFK"
BASE COUNT         2004 a         1381 c         1726 g         1878 t
ORIGIN      
        1 tgatctgatc aaagagtctc atacccgaat cattgcccgg tgcatcccgg gaagtcgtct
       61 ggttttgatc aaagggggta cacaccaggg cttttttaga aaaaaagaac gctatatagg
      121 agaaattctg aaatttattt ttgacgaaag atgttagtca aaactaacaa ctttatcaaa
      181 aaattaacat aattattaaa aaaataacga taacttttca tattgtatac aaagttaaag
      241 tgtgttatac tcaaaaatgg ctgttaggga catgctaaaa ttgttccatt aaacaaaaga
      301 aatcaaaaag aaagagggga agaaaccatg ggtttggcga cttttaaagg cggcattcat
      361 ccgtatgaag gaaaagaatt gtcagaaaac aaaccgatcc aggtccttct gccaaagggc
      421 gaactggtat ttcctatgtc tcagcatata ggagcacctg caaagccact ggtagcaaag
      481 ggtgacaggg ttcttgctgg acagaagatc ggagaagccg gtggatttat ttcagcaaat
      541 gtgatctgtt ctgtatccgg tactgttaag gcaatcgaac caagacgtat ggtaaatggc
      601 gcaatggtac cttccatcat cgtagaaaac gatggtgagt atgaaacagt agaaggagta
      661 ggagaggaca gagatccttc cacactctct aaagaagaga tccgttctat cgtaaaagag
      721 gcaggaatcg taggtttagg aggcgcaggc ttcccaactc acgtaaagct gactccaaag
      781 gatgagaatg cgatcgatta tgtaatcgta aacggcgcag agtgcgagcc atatctgacc
      841 agcgactatc gtatgctgat cgaagagcct gagaagatcg taggcggtct gaaggttatc
      901 ttacagctgt ttgataacgc caagggtgtg atcgcaatcg agaacaacaa gccggaggct
      961 attaagaaac tgacagagat ggtaaaggat gaacccaaga tcactgtctg tccgttactt
     1021 accaagtatc ctcagggtgg tgagcgttct atcatctacg ctgtaacagg acgcaaggta
     1081 aattcttcca tgctgccggc agatgcaggc tgtatcgtag ataacgtaga tacagttgct
     1141 tctatttata tggctgtatg caagtctact ccattaatga gaaagatcat taccattaca
     1201 ggtgatgctg tggcagaacc acagaacttc aatgtaaaac tgggaaccaa ttaccaggaa
     1261 ctgatcgaag cagcaggcgg tttcaagacc gagcctgaaa agatcatctc cggtggtcct
     1321 atgatgggac aggccctgtt tactgtagat gtgcctgtat gtaagacttc ttctgcttta
     1381 acctgtttta caaaagacca ggtagcagct tttgaaccaa ctgcatgtat ccgctgcgga
     1441 cgctgcgtaa gtgtctgccc aagccatatc gttccagtca tgatgatgaa agcagctatg
     1501 aaccgtgact gtgagacctt tgaaaagatc aacggtatgg aatgtatgga atgcggaagc
     1561 tgtacatata tctgtcctgc aagacgtcct ctgacccagg cattcaagga aatgcgtaag
     1621 acggttgcgg ctaaccgcag gaagaaaggc taggagggga caaaagctat gaataaatta
     1681 ttaaacgtat cttcatctcc tcatgtgaga tgcagtgtga atacacagaa cctgatgctg
     1741 gacgtagtga tcgctatgct tcctgcggca gctttcggtg tatataattt tggcttccac
     1801 gcactgcttg tacttttagt cacatcagca gtttgtgtgg caagtgaata tgtttacgaa
     1861 aaactgatgc acaagccaat taccattgca gacggaagtg ctcttgttac aggtatgatc
     1921 ctggcactga acatgccacc tcagatccct gtatgggtac ctgcactggg cggtatcttt
     1981 gcgatcatcg ttgtaaagca gctttacggc ggactgggac agaactttat gaacccggct
     2041 ctggctgcaa gatgcttcct gttaatttcc ttcgctggca tgatgaacaa cttctcagct
     2101 gcaagcctgg gatttgacag cgtttccgga gcaactcctc tggcagctat gcgtgccgga
     2161 cagagcgtag atctggcttc catgttcatt ggacgtatcc ctggtaccat cggtgaagtt
     2221 tctaccatcg ctcttctgat cggcggtatt tatctgttag tccgcaaggt aatctcctgg
     2281 agaattccgg tgatctacat tggaacagtt gcagtatttg tatttatctt tggtggtttt
     2341 gatgtaaatt atgtactctg ccaggtatgt ggtggtggtc ttatcttcgg tgctttcttt
     2401 atggcaactg actatgtgac cagcccgatc actccaatgg gacagatcgt attcggtgtt
     2461 gtcttaggac ttttaaccgg tatcttccgt ctgtggggtg cttctcctga aggtgtttcc
     2521 tatgcgatca ttttaagcaa cctgtttgta ccaatgatcg agcgtgtaac ccttccaaaa
     2581 gcatttggaa aggaggcaaa gaaggcatga aaggaatttt aaaagacgct gccatcctgt
     2641 ttgtcatcac tttagttgca ggactttgcc tgggcgcagt tcacgaagta acattagatc
     2701 cgatcgcaaa ggctcaggaa gcagcagcta ccaaaactta taaagaagta ttcccggaag
     2761 cagcaaaatt tgagcagacc gatgaactga cagcagcagt agcagcatct gcagatgaga
     2821 tcctggctca gggctttgga aatgtatctg ttgatgatgc acaggttgca gaagcagaag
     2881 atggttctgt tcttggatat ctgatcactg caagttccac agaaggctac aatggtctgg
     2941 tacagatctc tgtaggcatc acttctgagg gaaatctgac tggtcttggt ttcttaagca
     3001 tcagcgagac tcctggtctt ggtatgaagg caaaagaacc agatttcaag gatcagttca
     3061 atggaatgaa agcacagaaa ctggaagtaa caaagacaga cgcaacagct gataaccagg
     3121 ttcaggcgat cagtggcgct acctatactt ccaaggcagt aaccggtgcc acaaatgcag
     3181 ctatttactt tgtggaaaac tgcgttggaa agtaggaggt gtaagaaatg aacaagtata
     3241 cagaacgtct ttataatggt atctggaaag aaaatcctat tttcgttcag atgttaggtc
     3301 tgtgtcctac actggctgta actacatctg ctatgaacgg tcttggtatg ggtctttcct
     3361 ctaccgtagt attagtagca gcaaacttca ttatctccct gatgagaagc atcatcccgg
     3421 gaagagtccg tcttccaggt gagatcgtag ttgttgcttc cctggtaacc atcgtagaca
     3481 tgctgatgga aggctttaca ccagatcttt acagcgcact gggtatctac attcctctga
     3541 tcgttgtaaa ctgtgtaatc ttaggccgtg cagaggcatt tgctatgaag aacaaaccga
     3601 tcatctccgc attcgacgga gcaggcatgg gtcttggatt taccttcgcc cttgtttgta
     3661 tcggtgctgt ccgtgaactt ttaggtgcag gttccatctt tggtgtagcc attatgcctg
     3721 cttcctatca gcctatttcc atctttatcc tggcacctgg cgcattcttc gttctggctt
     3781 gcttaactgc tttacagaac aagttaaaga tgccttcagc aactaacggt tccgctaagg
     3841 gcaagaaggc taagaccgct tgctgtggcg attgcgcaaa ctgtgcagaa tcaaagacgg
     3901 aagaatagga ggtaaaaacg gatgaaagag ttacttgtta ttgccattag ctccgcattt
     3961 gtgagcaacg tggtattaag ccagttttta ggcatctgtt ccttcgtcgg cgtatctaaa
     4021 aaggtagaga cagcagcagg tatgggcggt gcggttatct tcgttatcac catcgcttcc
     4081 gttgttgcaa gtctgatcta tcagttcgtc ctgaacccat taggactgga ttatctgaag
     4141 accatcgtct ttatccttgt aattgcggct ctggtacaga tcgtagaaat gttcttaaag
     4201 aaatgtatgg tttccctgta caacgcatta ggtgtattcc ttccgctgat cacaaccaac
     4261 tgtgcagtat taggtgttgc acttactaac gtacagaacg gctacagctt tgtacagagt
     4321 gtagtaaacg gtgttgcaac tgctgttggt tatactgttt ccatcgtgct ccttgctggt
     4381 atccgtgagc gtattgaggg aaatgatatc ccatacaatt tccagggatc tcctatcgta
     4441 ctggtaactg caggtcttat ggcaatcgca tttttcggat tttccggatt actgtagaag
     4501 gaggagacga agatgaacat aacagcaatt ttgattgctg ctgcagttat tgcagtagca
     4561 ggtatcatcg taggaatcgg ccttggactt ttcggtgaga agttcaaggt agaagtagat
     4621 gaaaaggaag tagctatccg tgaggaactt cctggaaaca actgcggcgg ctgcggtttc
     4681 gcaggctgtg acgcgttggc aaaggcaatt gcagagggca aagctcctgc aaatgcatgt
     4741 cctgtaggtg gagcacctgt aggcgaaaag atcgctgcta tccttggcgt atctgccgga
     4801 gcagcagaga agaaggttgc atttgtacgt tgtaaaggta cctgtgacaa ggttaagact
     4861 ctttacaact acgaaggtat tgatgactgt gcaaaggcat ctgtagtgcc tgccggcggt
     4921 cctaaggcct gcagctacgg ctgtaccggt tttggttcct gtgtaaaggc atgtcagttt
     4981 gacgcgatcc atgttgtaga cggcgtagct gttgtagaca aggaaaagtg cgtagcctgc
     5041 ggcaagtgtg ttgctacctg tccaaaggga ctgatccaga tcgttcctta cagcgcagaa
     5101 catttagtac agtgcagcag ccatgacaag ggcaaagatg taaaggctgc atgtcaggct
     5161 ggatgtatcg gatgtacctt atgtaccaag cagtgtgagt cagatgctat ccatatggat
     5221 aacaacctgg cattcattga ttacgagaag tgtacaaact gcggcaagtg cgcggcaaag
     5281 tgccctgcta aggttattct gtaatttaag aaaaacagta tttaaaagag cttcagacgg
     5341 tgtgaaaata tcgtctggag ctctttttat tttgcttttg taaacgttct aataacaggg
     5401 cagagctgtg gcagccattt ccgttttcct taatggatga ttgtcataac cggatataca
     5461 taaaaaatta caaaaatgtt aaaagtgggg actacaccca ttttttgaca aaattataca
     5521 aggaaaatcg aaaaataaaa tcacgtaaaa tcaagggttt gtggccacta acagtttaca
     5581 taatatggct aaaaaacatt gtttataaat taacaaaatt aattattaac aaaaatgtaa
     5641 taaatacgta aaaaacaaat cgttataatg ctgatttgaa ggatgaaata aagaaataaa
     5701 tatacaaaac gcataaaata atttacaaaa atctccactg acaaataaaa cgtaacaaag
     5761 ttgttaccca atggttgacg tttaggattt aatcccatat aattaccgat acatcccaga
     5821 aaaacaggca taaagtgggg actacatccc acaatggcac ttgggagcgg atgcagttta
     5881 accgctctga aacggattta cgaggcagta tgcttcggga aagaatatga ggagtatgta
     5941 tgcttacatg gcattcattc cttcagtcgg tgatgttttt tgtacgttct atttctgtta
     6001 aggtgacaaa gaaccattac cgttcgtctt ctgtccttat gtcaggagcc gttgtgattg
     6061 cagtagtcgc tttttcagcc aatggatttg gcggaggcgg aaagaacgca atggcagcgc
     6121 ccagaaccgt agactctctg gaagcagatg aggaaccgga gacagaggca ctttttgaag
     6181 gaagcagttt tgttacagaa gcaaaaatac aattcggact tttgaacaca ggcagtgaag
     6241 gtcagcatct ggccggtgcg ctgttggaga gagatgtccg ggacagacag cgcaggcagg
     6301 cagatgcgca gatagaaata gaagcgcttc agaaacagat attaaaagaa aaagaagagg
     6361 ctgaagcaag acggaaagca gaggaagaga gaagagccgc cagaaggatc aaagtgtctg
     6421 atgaagatta tcaggtatta cttcggatcg ttcaggcaga agctggtgta tgtgatgaga
     6481 aaggcaagat cttagtggcc gatgtgatca tcaaccgcgt tctttcagga aagtttcctg
     6541 gaagcgtaaa gggtgttgta taccagggtt cccagttcca gccggtgtcc aacggcagga
     6601 ttaattccgt aaaagtaaca gctgaaacca ttcggtgtgt ggaccgtgca ctggacgggg
     6661 aagattattc caacggtgcg ttatatttta tgaacagaaa agcttccggc agaaaaacag
     6721 gctggtttga ctccagatta acctaccttt tcgcacacgg aggtcacgaa ttttttaaat
     6781 agttcagcca tgcgctggct tgaatgaggg agtgtgttgc aagcttgctt gcataagcac
     6841 tttctcattc gaaccagcat atggtggaac cacaaggctt cttgttttca tgaatatgga
     6901 aacaagaaga tgtcatggct ggactgtaat tagttttttt aaatagctgt aagaaaggga
     6961 ttgagtttca ggctgctttg ggtgtatac
//
LOCUS       DBMDUA010000150         6908 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4637_length_6908_cov_2.630087, whole genome shotgun sequence.
ACCESSION   DBMDUA010000150 DBMDUA010000000
VERSION     DBMDUA010000150.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6908)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4637_length_6908_cov_2.630087"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(31..1845)
                     /gene="lepA"
                     /locus_tag="ACZPCL_10380"
     CDS             complement(31..1845)
                     /gene="lepA"
                     /locus_tag="ACZPCL_10380"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR1162909.1"
                     /translation="MAVIDQSKIRNFCIIAHIDHGKSTLADRIIEKTGLLTSREMQEQ
                     VLDNMDLERERGITIKSQAVRTVYRAKDGNEYIFNLIDTPGHVDFNYEVSRSLAACDG
                     AVLVVDASQGIEAQTLANVYLALDHDLDVFPVINKIDLPSADPDRVAAEIEDVIGLEA
                     HDAPKISAKTGLNVEEVLEAIVHKIPAPKGDPKAPLQALIFDSVYDSYKGVIIFCRVM
                     EGTVKKGTPIRMMATGAEEEVVEVGYFGAGQFIPCDELAAGMVGYITASIKNVRDTRV
                     GDTVTDSSNPCAAPLPGYKKVTPMVYCGLYPADGAKYNDLRDALEKLQLNDASLFYEP
                     ETSVALGFGFRCGFLGLLHLEIIQERLEREYNLDLVTTAPGVVYRVHKTTGEMIELTN
                     PSNLPDPTEIEYMEEPIVSAEIMVTTEYVGAIMTLCQERRGVYLGMEYIETTRALLKY
                     ELPLNEIIYDFFDALKSRSRGYASFDYELKGYERSELVKLDILVNREEVDALSFIVHA
                     DSAYEKGRRMCEKLKDEIPRHLFEVPIQAAIGGKIIARETVKAVRKDVLAKCYGGDIS
                     RKRKLLEKQKEGKKRMRQIGNVEIPQKAFMSVLKLDEN"
     gene            complement(2110..3330)
                     /gene="spoIIP"
                     /locus_tag="ACZPCL_10385"
     CDS             complement(2110..3330)
                     /gene="spoIIP"
                     /locus_tag="ACZPCL_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061783 - peptidoglycan muralytic activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HIR1162910.1"
                     /translation="MKSWSRNVKKWILFLKITGILVILCMIAGACILYAAENHIQLLK
                     NNLSNEVWESGCEWVWQQIFPLSMDLTGKHSNQTLQTQKKWSSINTMDPAYDQYEKAR
                     EFYRTHEYLAWIGEDESDEETIRSASEEALDQSENANSPANTDSASAKISDQPMIYPM
                     QQLMDYDFLMKHFYNVHTSTTADRELMNAKNLLSKDLTLEKEDAPQILIYHTHSQESY
                     KNSKKDENVVAVGQKLTDLLTEKGWNVYHDTTVYDLAVGKLDRSRAYTYALEGINEIL
                     ARYPSIQVVLDIHRDGVGEDVYLKTEIGGQPSARIMFFNGLSQTPEGPISYLANPFRE
                     DNLAFSLKLQLKAEEYYPGFTRKIYLKGLRYNQHLRARSALVEVGAQTNTFEEACNAM
                     MPLAELLDMVLQGK"
     assembly_gap    3483..3502
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3503..4343)
                     /locus_tag="ACZPCL_10390"
     CDS             complement(<3503..4343)
                     /locus_tag="ACZPCL_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1162911.1"
                     /translation="MSAQKTKAAEHLAAIFTMLIWGTTFISTKVLLEDLSPLEILVLR
                     FVAGYIFLWIIRPVPLKAGSLKQEGIMIVAGITGIGIYYLMENIALTLTQASNISVIV
                     SIAPIFTGILAHFFLEGEKMKKSIVLGFVCAMAGICLITFQKGETVELNPLGDFLGVA
                     AAAIWGVYSICSRKIAGFGYDTIITTRRTFFYGILFMLCALPFMDFHLSLSALLKPVN
                     LGNIIYLGVGASAICFVTWNYAVKSLGATRASVYIYGIPVVTILMSIPLLHEVITFQA
                     MAGT"
     gene            complement(4391..5296)
                     /gene="gpr"
                     /locus_tag="ACZPCL_10395"
     CDS             complement(4391..5296)
                     /gene="gpr"
                     /locus_tag="ACZPCL_10395"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HIR1162912.1"
                     /translation="MKIPEFKIRTDLALEEREGLKEAAEEVSGVSFKKWEGKRSAAQI
                     TEVKVLNEHGAKALGKPMGSYLTLEADKLLMPDEDFHSEVSDELALLLKGLLKQKTGK
                     NILVAGLGNQSVTPDSLGPRTVSNLCVSAEGLKAIAPGVMAQTGMETARILLGIIKEV
                     QPDLVIAIDALAARSIRRLGTTIQLTDTGIHPGSGVGNHRHSLTKETLGIPVLAIGVP
                     TVVGAAAIVHDTISALLGVLSSQAETKNTGKWIQNMDPEDQYRLITELLEPEFGSLYV
                     TPPDIDERVRQLSFTISEGIHRALF"
     gene            5623..5886
                     /gene="rpsT"
                     /locus_tag="ACZPCL_10400"
     CDS             5623..5886
                     /gene="rpsT"
                     /locus_tag="ACZPCL_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR1162913.1"
                     /translation="MANIKSAKKRILVNETKAARNKAIRSKVKTAIKKVEAAVAAGDK
                     AAAQAALVAATTEIDKACTKGVYHKNNAARKVSRVSKAVNSIA"
     gene            complement(6107..6712)
                     /locus_tag="ACZPCL_10405"
     CDS             complement(6107..6712)
                     /locus_tag="ACZPCL_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HIR1162914.1"
                     /translation="MAEEKNIPGVATEKESDEVLEEIRTQTKEVLKELLAVAKMKPGQ
                     ILVVGCSSSEIGSYKIGSHSSETIGQTVYKALYEELKPLGIYLAAQCCEHLNRSLILE
                     EEAAEKYGYEPVNVVPQLKAGGSFSTAAYHTLEHPVAVEHIKAHAGIDIGDTLIGMHM
                     RDVAVPVRIQTKAIGGAHVVCARTRLKFVGGIRARYDEEQM"
     gene            complement(6736..6867)
                     /locus_tag="ACZPCL_10410"
                     /pseudo
     CDS             complement(6736..6867)
                     /locus_tag="ACZPCL_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
BASE COUNT         1755 a         1712 c         1366 g         2055 t
ORIGIN      
        1 gcgccgacag gccggtctca tttttatctc ttaattttca tccagcttca gcacgctcat
       61 aaaggccttc tgcgggatct ccacattgcc gatctgacgc atgcgcttct ttccttcctt
      121 ctgcttctct aacagctttc tcttacgtga aatatcaccg ccgtaacatt ttgcaagtac
      181 gtccttgcgc actgccttaa ccgtctccct ggcaatgatc tttccgccga ttgccgcctg
      241 gatcggtacc tcaaacaggt gtctcgggat ctcatccttt aacttttcgc acattctcct
      301 gcccttttca taagcagagt ctgcatgtac gataaaggaa agggcatcca cttcttccct
      361 gtttacaagg atatccagtt ttaccagttc ggaacgctca tagcccttta actcatagtc
      421 aaaagaagca tagcctctgg aacgtgattt taaagcgtca aagaaatcgt aaatgatttc
      481 atttaacgga agttcatatt ttaataaggc tctggtagtc tcaatgtatt ccattcccag
      541 atatacgccg cggcgctcct ggcacagtgt cataatggca cctacatatt cggtagttac
      601 catgatctct gcgctgacga tcggctcttc catgtactcg atctcagtcg gatccggaag
      661 gttagacggg ttggtcagtt caatcatctc tcctgtggtc ttgtgtacac ggtatacaac
      721 gcctggtgct gttgttacaa ggtccaggtt gtattcacgt tccagacgtt cctggatgat
      781 ctctaaatgc agcagtccta aaaatccgca gcggaaacca aagcctaaag caacagaggt
      841 ctcaggctcg tagaacaggg atgcgtcatt taactgcagc ttttccaatg catcacgcag
      901 atcattgtat tttgcaccgt ctgcaggata cagtccgcag taaaccatag gtgtaacttt
      961 cttgtaacct ggcagaggcg ctgcacatgg attgctgctg tctgttactg tatcacctac
     1021 acgggtatca cggacatttt taatgctggc tgtaatatag ccaaccatac cagctgccag
     1081 ctcgtcacat gggataaact gaccagctcc gaaatagcca acttctacaa cctcttcctc
     1141 tgctcctgtt gccatcatac ggatcggtgt gcccttcttt accgttcctt ccataacacg
     1201 gcagaagatg ataacaccct tgtaggagtc gtaaacagag tcaaagatca gtgcctgtaa
     1261 aggcgctttt ggatctccct ttggcgccgg aattttgtga acgatcgcct ctaatacctc
     1321 ttccacgttc agtccggtct ttgcggagat cttcggggca tcatgtgcct ccaggccgat
     1381 cacatcttcg atctcagctg ccacacggtc cggatcagca cttggaaggt caatcttgtt
     1441 gataaccggg aatacatcca gatcatgatc caatgccaga tatacattgg ccagagtctg
     1501 ggcctcaatt ccctgggaag catctactac cagcacagca ccgtcacagg ctgccaggct
     1561 tcgtgacacc tcataattaa aatccacatg tccaggtgtg tcgatcaggt taaaaatgta
     1621 ttcatttcca tcttttgccc tgtatacagt acgcacagcc tgggatttaa tggtaattcc
     1681 acgttcacgc tccaggtcca tattgtcaag gacctgttcc tgcatctcac ggctggtaag
     1741 aagtcctgtt ttctcaatga tacggtctgc cagtgtggat ttaccgtgat ctatatgagc
     1801 aatgatacaa aaatttcgta ttttactctg gtcaattact gccattcttt atcctctttc
     1861 tttcaattta gatgttgggg tcaaaaggta ttttgacccc tctgataccg aattgctacg
     1921 cgctttgcac tctcgcaatt ctaaaaacat tcgtaatccg ctcatttttc ttcaaaaaat
     1981 ggctccttac tcatgttttg gcgggtccca agttcaaaaa tcctcctgca agcaagcttg
     2041 cgtcggattt ccgacctttt gcccctggta tctcaatcta tagaatatcg taactatttt
     2101 actatgtttt cattttcctt gcaacaccat atccaaaagt tctgccaggg gcatcatcgc
     2161 attgcaggcc tcctcaaagg tattggtctg ggctcccacc tccaccagtg ctgatcttgc
     2221 ccgcaggtgc tggttataac gcagtccttt taaatagatc tttctggtaa aaccaggata
     2281 atactcctct gcttttaact gcagtttcag gctaaatgcc agattatctt cccgaaaagg
     2341 atttgccaga tatgaaatgg gtccttccgg cgtctggctc agaccattaa aaaacatgat
     2401 cctggcactt ggctgaccgc ctatctctgt tttcaggtat acatcctccc ctacaccgtc
     2461 ccgatggata tcaagcacta cctggatcga tgggtaccgg gccaggatct cattaattcc
     2521 ttccagtgca taagtgtacg cacggctgcg gtccagcttg cccactgcca gatcatatac
     2581 cgttgtgtca tggtaaacat tccagccttt ttctgtaaga agatccgtca gtttctgtcc
     2641 cactgcaacc acattttcat cttttttact gtttttgtaa ctttcctggg aatgggtgtg
     2701 atagatcagg atctgaggcg catcttcctt ttccagggta agatcctttg acaacaggtt
     2761 cttagcattc atcagttctc tgtctgctgt agtagaagta tgtacattat agaagtgttt
     2821 cattaaaaaa tcataatcca tcaactgctg catgggatag atcatgggct gatccgaaat
     2881 tttagcagaa gcgctgtccg tgtttgcagg cgaattggca ttttcagact gatccaatgc
     2941 ttcttcgctg gctgaccgga ttgtttcctc atctgactca tcttccccga tccaggccag
     3001 atattcatgg gtgcggtaaa attcccttgc tttttcatac tggtcatagg ccggatccat
     3061 ggtgtttatg gatgaccatt ttttctgtgt ctgtaatgtc tgatttgaat gttttcctgt
     3121 aagatccatg cttaagggaa atatctgctg ccagacccat tcacagcctg actcccagac
     3181 ttcatttgaa aggttgtttt ttaataactg tatgtggttt tctgctgcat acaaaataca
     3241 ggctcctgca atcatacata ggatcaccag gatgcctgtt atcttcagaa ataatatcca
     3301 tttttttaca tttctgctcc atgatttcat ggtgtccttc cagttaaaaa gaaaatattg
     3361 gcaccgggga aacaaatgcc gccttcggca ccccttgttt ttccctttat gtcatatata
     3421 tgcagatcat cctgccggaa gttcatcaca tttcttattt tttaggagaa aatggccact
     3481 cgnnnnnnnn nnnnnnnnnn nnccgttcct gccattgcct gaaaggtgat cacttcatgg
     3541 agaagaggga tcgacattaa aatcgttaca accgggatcc cgtaaatata tacgctggct
     3601 ctggtagcac ctaaggactt tactgcataa ttccaggtga caaagcagat tgcagaggcg
     3661 cctaccccca gataaatgat atttcccaga tttaccggct ttaataatgc ggaaagggaa
     3721 agatggaaat ccataaaagg aagggcacac agcatgaaca aaataccata gaaaaaagtt
     3781 ctccgggtgg taatgatggt atcatatcca aaaccggcta tttttctgga acagatggaa
     3841 tatactcccc agatggctgc cgctgccaca ccaaggaaat ctcccagtgg atttaactct
     3901 actgtctccc ctttctggaa tgtgatcaga cagatgcctg ccattgcaca cacaaatccc
     3961 agaacgatac tcttcttcat tttctcccct tccaggaaaa agtgggccag aatacctgta
     4021 aaaatagggg cgatcgatac aataacgctg atattcgatg cctgtgtcaa agtcagggca
     4081 atgttttcca taagataata aataccgatc cccgtaatac cggccacgat catgatccct
     4141 tcctgtttca ggcttcctgc tttcaaagga accgggcgga taatccacag gaaaatataa
     4201 cctgccacaa accgcagcac cagtatttca agaggtgaca gatcttctaa caggactttc
     4261 gtcgatataa acgttgtccc ccagatcagc atagtaaaga tcgcagccag atgttcggct
     4321 gcttttgtct tttgtgcact catttctttg ctccttatcc cttttcatat ttatatacta
     4381 tataagccta ttaaaataaa gccctgtgga tcccttctga aatagtaaaa cttaactggc
     4441 gtactctctc atcaatatcc ggcggtgtta cataaagaga accaaactcc ggctctaaaa
     4501 gttctgtgat cagacggtac tgatcttccg gatccatgtt ctggatccat tttcccgtat
     4561 ttttcgtttc tgcctgggat gacagtaccc caagcaaagc ggagatcgta tcatgaacaa
     4621 tggctgccgc acctactacg gtaggaactc caatcgcaag aaccggtatt cctaacgttt
     4681 cttttgtaag actgtgtctg tgatttccaa ctcctgaccc gggatgaatt cctgtatccg
     4741 tcagctggat ggtggttcca aggcgccgga tgctgcgggc tgccagagcg tcaatggcga
     4801 tcaccagatc cggctgtacc tccttaataa tgcccaacag gatcctggca gtttccattc
     4861 ccgtctgggc cataacaccg ggagctatgg cttttaaccc ttctgcagac acgcagagat
     4921 tactgaccgt ccggggccca agggagtccg gggttacgct ttgatttcca agccctgcta
     4981 ccaggatatt tttcccggtc ttctgcttta acagtccttt taacaacaac gccagttcat
     5041 cagatacttc actatgaaaa tcttcatcag gcataagcag tttatctgct tccagtgtaa
     5101 gatagcttcc catcggtttt cccaaagctt tggcgccatg ttcatttaaa accttcactt
     5161 ctgtgatctg ggctgctgac cgttttcctt cccacttttt aaaagaaacc cctgaaactt
     5221 cttccgctgc ttcttttaaa ccttcccgtt cctctaaagc aaggtctgta cgtattttaa
     5281 attcaggtat cttcattttt cgctgcctcc ttgttttgtc ctgtataact gtaacgatcg
     5341 ttaaaattag tttttacaca gagacagcgt attatgccac atggatttta ttataactta
     5401 cagaaataga actggcaatc agaaacattt cctgcattgg ttcatgatac tgtagaccgt
     5461 ctgcttaaca ttcttgataa tttttaaaat ttttacttga catttccaaa tggatccgat
     5521 agaatgtaac agtatgttta cattgaactg tccgtgtcca ggctttgatg gaaaacacaa
     5581 cgattaatat ttttatataa taccgaacgg aggtgtattt cattggctaa cattaaatct
     5641 gcaaagaaga gaatcttagt aaacgaaact aaggctgcaa gaaacaaggc gatcagatct
     5701 aaggttaaaa ctgcgatcaa gaaagttgag gctgctgtag ctgctggaga taaggctgct
     5761 gctcaggctg cactggttgc tgctactacc gagatcgata aggcttgcac caagggtgta
     5821 tatcacaaga acaatgctgc ccgcaaagtt tctcgtgtat ccaaggctgt taattctatc
     5881 gcttaattta tagaattcga tcaatcgata ccgtaattta aaaactctcc ggattaaccc
     5941 accggagagt ttttttgtta ttcagagcaa tgcgaaaatg cagggaaaag tgtggatcat
     6001 gcttgcatga atgaacactt ttccctgcat tttcatatga cgagcagcca catcgagatg
     6061 cagcgcagcg aaatcgagat gtttgctctg aataattgta ctttccctac atctgctctt
     6121 catcataacg tgctctgatg ccgcctacaa atttcaaacg ggtccttgca catactacgt
     6181 gggcaccacc aatggccttt gtctgaattc ttacaggcac agccacatct ctcatatgca
     6241 taccgatcag agtatctcca atatcaattc ctgcatgtgc cttgatatgt tctactgcaa
     6301 ccgggtgttc cagggtatga taagccgcgg ttgagaaaga accacctgcc tttaactgag
     6361 gaactacatt gaccggttca tagccgtatt tctccgctgc ctcctcttct aggatcaggg
     6421 aacggtttaa atgctcgcag cactgcgcag ccagataaat tcccaatggc tttaattctt
     6481 catacagtgc tttgtataca gtctgtccaa tggtctcact ggaatgggag ccgatcttgt
     6541 aacttccgat ttcgctggaa gaacagccta ctaccaggat ctgacctggt ttcatcttgg
     6601 caacagccag aagttccttt aaaacttcct tggtctgtgt gcggatctct tctaacactt
     6661 catcactttc tttttccgtt gctacccctg gtatattttt ttcttctgcc atttaatgca
     6721 ttctcctcct taattctaat atttattagc gatcagcagt tccactgcca gacggtcctg
     6781 gagttttccc gttttcacac tttcttctgc gtccacacac aaattcacat aggacaagat
     6841 ctgttccttc gtaaatgttt tcgcctgcga acttttgcaa agttaagaaa agcaaatgaa
     6901 tataccaa
//
LOCUS       DBMDUA010000151         6839 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4693_length_6839_cov_3.047640, whole genome shotgun sequence.
ACCESSION   DBMDUA010000151 DBMDUA010000000
VERSION     DBMDUA010000151.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6839)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4693_length_6839_cov_3.047640"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            159..707
                     /locus_tag="ACZPCL_10415"
     CDS             159..707
                     /locus_tag="ACZPCL_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HIR1162915.1"
                     /translation="MANIIVAFSKPEDGKSIKGILVRSGYEVAAVCMSGSQVLSAAED
                     LSGGIVVCGYRFEDMMYDELRQCLSSNFEMLVLASPARWSGIQMSGVVCLPMPLKVHD
                     LLSTLDMMLQAQQRIRRKLRSRPRERSKEEQDLINEAKALLMDRNNMTESEAHRYIQK
                     CSMDSGTNLVETAQMIISLIHV"
     gene            738..1580
                     /gene="dapF"
                     /locus_tag="ACZPCL_10420"
     CDS             738..1580
                     /gene="dapF"
                     /locus_tag="ACZPCL_10420"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR1162916.1"
                     /translation="MEFTKMQGIGNDYVYVDCFKEKVEHPGEVSKYVSDRHFGIGSDG
                     LILICPSDKADCRMDMYNADGSQGIMCGNGVRCVGKYVYDHGIVDKDRRIITVETLGG
                     IKTLDIQVEDGKAVMLTVDMGEAELTSKLPEKIVIDGTEQEFTGISVGNPHAVYFTEG
                     INDLDLEKIGPSYENHPRFQPARVNSEFIEVKSRDHIRMRVWERGSGETWACGTGATA
                     SAMAAILRGFTDDEVRVSLRGGDLLIRFDRESRHLFMTGPAKEVFHGSIDIPEEIYYD
                     LSEK"
     gene            1703..2923
                     /locus_tag="ACZPCL_10425"
     CDS             1703..2923
                     /locus_tag="ACZPCL_10425"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HIR1162917.1"
                     /translation="MFRVNENYLKLPGSYLFSTIAKKVAAYQKENPDKKLIRLGIGDV
                     TLPIAPAIIDAMHKAVDEMGHAETFHGYAPDLGYAFLRETIAKKDFQERGCQIEADEI
                     FVSDGAKSDTGNIQEIFSADSKIAVCDPVYPVYVDTNVMAGRTGEYDTEKGTWSNVIY
                     MPCTAENGFVPELPKETPDLIYLCVPNNPAGTTLTKDQLKVWVDYANEKDAVILYDAA
                     YEAYISEDDVPHSIYEIPGARTCAIEFRSFSKKAGFTGVRLGYTVIPKDLKSGDVMLH
                     SLWARRHGTKFNGAPYIEQRAGEAVYSEEGNKQVMEQVAYYKRNAKVIYDGLKEAGYT
                     VFGGVNSPYVWMKVNEGMTSWEFFDYLLSEANVVGTPGSGFGPSGEGYFRLTAFNTYE
                     NTVEAVERMKALKK"
     gene            2961..3401
                     /locus_tag="ACZPCL_10430"
     CDS             2961..3401
                     /locus_tag="ACZPCL_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR1162918.1"
                     /translation="MDDIDRKILKLLQENARMSLKTIAEKTFLSSPAVSARIEKLEKD
                     GVIAGYHAMVDPMKLGYHILAFINLDVIPEDKPKFYAFAESVPNVLECSCVTGEYSML
                     MKVAFHSTMELDIFIGQLQKFGKTSTQIVFSTHVGPRGVDVDEV"
     gene            3461..4243
                     /locus_tag="ACZPCL_10435"
     CDS             3461..4243
                     /locus_tag="ACZPCL_10435"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR1162919.1"
                     /translation="MTGNEKVILDAGHGGDEPGAIYNGRKEKDDTLRLTLAVGRILEE
                     NGVSTLYTRTTDVYDRPQEKAEIANRSGADLFVSIHRNAMPVPGTGSGASVLVYEDSG
                     TAAVLAENILKNLVELGLKDQGIEERPGLVVLRKTQMPAVLAEVGFIDNPSDNAFLDR
                     NFDAIARAIADGILTTLREQKEQTPLYYQVQTGAFRDRGRAMRLAGELQSAGFPAFIV
                     YDEGLYKVRVGAFLDMENGIRMEQNVKRAGYPAYLVREAAIY"
     gene            complement(4268..4603)
                     /locus_tag="ACZPCL_10440"
     CDS             complement(4268..4603)
                     /locus_tag="ACZPCL_10440"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1162920.1"
                     /translation="MDETFMKARAYLAQNYSDPELSLTDLCHYLGFSCSYFSTLFKQK
                     TGLNYSHYITSLRLEQAARLLTDTDDTALSIGYQVGYSAPNYFSRAFKKFYGISPSRY
                     REQNRVPTV"
     gene            4750..5640
                     /locus_tag="ACZPCL_10445"
     CDS             4750..5640
                     /locus_tag="ACZPCL_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1162921.1"
                     /translation="MQIITNKSQKELKEHGSFQFPVLVSRECLSGYETGAFLWHWHPE
                     VELTLVTDGVIEYHVNGNTYILKERQALFGNANSLHSGGMVEGQDCHYISITFDPKLI
                     YGYENSLIYEKYVMPVVRDGALCAVVFDGSQEWHGEMIELIRGIISAYDGQEDGYEME
                     LTGKLLKFWQLLVLNVDRKKGISGKEQKDYERIREILRFIEKNYDKKLTVDQIAENVY
                     MCTSQCSRLFKKYMKKTLFDFILEYRIERSLADLLDPGCSMAEAAAGAGFNDSNYYSK
                     VFVRIKGCAPSVYRKKMRGR"
     gene            complement(5708..6097)
                     /locus_tag="ACZPCL_10450"
                     /pseudo
     CDS             complement(5708..6097)
                     /locus_tag="ACZPCL_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867203.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
     gene            complement(6137..>6839)
                     /locus_tag="ACZPCL_10455"
     CDS             complement(6137..>6839)
                     /locus_tag="ACZPCL_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
                     /protein_id="HIR1162922.1"
                     /translation="EQAQERFNKEYTPRNAKPSEVTKHWLSGLLKCSGCGRSLSSCVV
                     HRKSLPDTFYFQCYGYLKGKCSTNCYVNESVIGPAVLNALKDALNNGSVSYSLRQRQT
                     DHAPDAEIKNIEKKLSRLDSKELRAKEAYIDGIDTIEEYRANKERISQERTELEEQLA
                     KLTQVPEEPEDNTRKMLQSISSILDILQSDSFTPQEKNAALKSIVEKITYDRANSHID
                     VDFYLTENPEMPANP"
BASE COUNT         1950 a         1284 c         1829 g         1776 t
ORIGIN      
        1 gcaagtttac ttgcataagc actttttcat ccaaatcagc atatggtgga cccaccaagc
       61 ttcttgtttt catgaatatg gaaacaagaa gatgccatgg ctgaattgta aataacagtt
      121 acaagcagga aaactaggtt tacccggagg tgagacatgt ggctaatata atcgtggcat
      181 tttccaaacc ggaagatgga aaaagcataa aaggtattct ggtaagaagc ggatatgaag
      241 tggctgctgt ctgcatgtca gggtcacagg ttctttcagc tgcggaagat ctgagcggtg
      301 ggatcgtagt ctgcggatac cggtttgaag atatgatgta cgacgaactg agacagtgcc
      361 tttcttctaa ctttgagatg ctggtgctgg catctcctgc aaggtggagc gggatacaga
      421 tgtccggggt ggtatgtctt cctatgcccc ttaaggtgca tgatctgtta agtacgctgg
      481 atatgatgct ccaggcgcag cagcgcattc gcaggaaact gcgcagcagg ccaagggagc
      541 gcagcaagga agaacaggat ctgatcaatg aggcaaaggc gcttttgatg gaccgtaaca
      601 atatgacgga gtcggaagcc cacagatata tacaaaaatg cagtatggac agtgggacca
      661 atctggtgga aacggcccag atgatcatta gtctgatcca cgtataagag aatgcaggat
      721 aatgtatgag gtgtgagatg gaatttacaa aaatgcaggg aattggcaat gactacgtgt
      781 atgtagattg ctttaaggaa aaagtggaac atccggggga agtgtccaaa tatgtcagcg
      841 accgccattt tggcattggt tctgatgggc tgatcctgat ctgcccgtct gataaggcag
      901 actgccgtat ggatatgtac aatgcagatg gttcccaggg aattatgtgt ggaaatggtg
      961 tacgttgtgt aggaaaatac gtgtatgatc atggaatcgt agataaggac agacggatca
     1021 ttactgtgga aacattagga ggtatcaaga cattggatat ccaggtagaa gacggaaaag
     1081 cagtgatgct gactgtagat atgggagaag cagaactgac ttccaaactt ccggaaaaga
     1141 tcgtaattga cggtacagaa caggaattta caggaatcag tgtgggaaat cctcatgcag
     1201 tctattttac agaaggaatc aatgacctgg atctggaaaa gatcggcccg tcctatgaaa
     1261 atcatccacg tttccagccg gcccgtgtaa attctgaatt tatcgaagta aagagcagag
     1321 accacatccg catgcgtgtg tgggagagag gcagcgggga aacctgggcc tgcggtacag
     1381 gagctacagc cagtgccatg gcagctatct taagaggttt tacagatgat gaggtgcgtg
     1441 tatctctacg gggcggcgac ctgctcatcc gctttgaccg ggaaagcagg catctgttta
     1501 tgacaggacc tgcaaaagaa gtatttcacg gaagtattga catcccggaa gaaatatatt
     1561 atgatttatc cgaaaaataa atatgcctat attctttcag agaatatagt aaacctgtaa
     1621 aatatacact ctcaggcctt ggatctacga cgttgtgggt tcaaaggtcc tgggagcgtt
     1681 tgcgttaatg gaggatgaaa gtatgtttag agtgaatgaa aattatctga aactgccggg
     1741 aagctacctg ttttcaacca ttgcaaaaaa agtagctgct taccagaaag agaacccgga
     1801 taagaaactg atccgtctgg gaattggtga tgtaaccctt cctatcgcac cggcgatcat
     1861 tgatgctatg cataaggcag ttgatgaaat gggacatgct gaaacctttc acggatatgc
     1921 acctgatctg ggatatgcat ttttaagaga gaccattgca aagaaggact tccaggaaag
     1981 aggatgccag atcgaggcag atgagatctt cgtttctgac ggtgcaaaga gcgataccgg
     2041 taacattcag gaaatcttca gcgcagacag caagattgca gtttgcgacc cggtttatcc
     2101 tgtttatgtg gataccaatg ttatggccgg ccgtaccggt gagtatgata cagaaaaggg
     2161 aacctggagc aatgtgatct acatgccgtg tactgcagag aacggttttg ttccggaact
     2221 tccaaaggaa acaccggatc ttatttatct ttgtgttcca aataacccag caggaactac
     2281 ccttacaaag gatcagttaa aggtatgggt tgattacgca aatgaaaaag acgctgtaat
     2341 tttatatgat gcagcgtatg aggcttatat ttctgaggat gatgttcctc acagtattta
     2401 tgagatcccg ggagcaagaa cctgtgccat tgagttccgt tctttctcaa agaaggccgg
     2461 atttacaggt gtccgtttag gatataccgt tatcccgaaa gacttaaaga gcggtgatgt
     2521 aatgcttcat tctttatggg caagacgtca cggaaccaag ttcaacggtg ctccttacat
     2581 tgagcagaga gcaggagaag ctgtttactc agaagaagga aataaacagg tcatggagca
     2641 ggttgcttac tataagagaa atgcaaaagt gatctatgac ggattaaagg aagcaggtta
     2701 tactgtattt ggtggtgtga attcccccta tgtatggatg aaggtcaatg agggaatgac
     2761 cagctgggag ttctttgatt acctcctttc tgaggcaaat gtagttggaa caccgggaag
     2821 cggctttgga ccaagcggag aaggatattt ccgtctgact gcatttaata cctacgaaaa
     2881 tacagtagaa gcagtagaac gtatgaaagc gttaaagaag taatagaacg aaccgaacac
     2941 aggaggaagt cttaatgctc atggatgata tcgaccgtaa aatattaaaa cttttgcagg
     3001 aaaatgccag gatgtcttta aagaccattg cagaaaaaac ctttctgtca tctccggctg
     3061 tctctgcaag gatcgaaaaa ctggaaaaag acggggtgat cgccgggtat catgccatgg
     3121 tagaccccat gaaattaggg tatcacattt tggcttttat caacctggat gtgataccgg
     3181 aagataaacc aaagttttac gcatttgcgg agtctgtacc aaatgtactg gaatgcagct
     3241 gtgtaacagg agagtattcc atgttaatga aagttgcttt tcacagtacc atggagctgg
     3301 atatttttat tggacagctt cagaaatttg gaaagaccag tacacagatc gtattctcca
     3361 cccacgtagg cccaagaggt gtggatgtgg atgaagtgta acattcataa gctccctcac
     3421 atatggtata gagacagaca tatgtgaggg agcttgtttt atgaccggaa atgaaaaggt
     3481 cattctggat gcgggacatg gcggggatga gccaggtgcc atttataatg gaagaaagga
     3541 aaaagatgat accctgcgcc tgactcttgc ggtagggcgt attttagagg aaaatggagt
     3601 aagcacattg tatacaagga ctacagatgt atacgaccga cctcaggaaa aggcagagat
     3661 cgccaaccgg tccggagcgg atctgtttgt ttccattcac cgcaatgcca tgccagtgcc
     3721 gggaacagga agcggggcat ctgtactggt atatgaggac tccgggacag cagcagtcct
     3781 ggcagaaaac attttaaaaa atctggtgga actgggattg aaagaccagg ggatcgagga
     3841 acggccggga ctggtagtcc ttagaaagac ccagatgccg gcagtgttag cagaggtggg
     3901 atttattgat aatccttcgg ataatgcttt tcttgaccgg aattttgacg ccattgcaag
     3961 ggcaatcgca gatgggatac tgactacttt gcgggaacag aaagagcaga cgcctcttta
     4021 ttaccaggtc cagacaggtg catttagaga tcgtggaagg gccatgagac tggcgggaga
     4081 gctgcagtcg gcaggttttc ctgcgtttat tgtatatgat gagggattat acaaagtccg
     4141 tgtgggagca tttttagata tggaaaacgg gatccgcatg gaacagaatg taaaaagagc
     4201 aggttatccg gcatatctgg tgcgggaggc tgcgatctat taagaaaaga gttcctgccg
     4261 ttggatatta cacggtagga actctgtttt gttctctgta acgggatggg gagatcccat
     4321 aaaatttttt aaaagcacgg ctgaagtagt tgggggcgga gtagcctacc tgatagccga
     4381 tggaaagagc cgtatcatct gtatctgtta aaagcctggc tgcctgttct aagcgcaggc
     4441 tggtaatata atggctgtag tttaaaccgg tcttctgttt aaaaagtgtg gaaaaatagg
     4501 agcagctaaa accaagataa tggcacaggt ctgtaaggga cagttccggg tcagaatagt
     4561 tttgtgcaag ataggctctg gctttcataa aagtttcatc catgttagaa cacgctcctt
     4621 tttgcatgat ggcctgtttt cctgttgcaa cagtgtagac aggtaagata cttgtattat
     4681 ataataaaaa taagcagttt tccttgaata tattgctaaa aacaggtaat atcctgcgcg
     4741 gaggtcctta tgcagatcat aaccaataaa tcccagaaag agttaaaaga acatggaagt
     4801 tttcagttcc ctgttttagt tagcagggag tgtctttccg gctatgaaac aggggctttt
     4861 ctctggcact ggcatccgga ggtggaactg acccttgtta cagatggggt gatcgaatac
     4921 catgtaaacg gaaataccta tattttaaaa gagagacagg cgttgtttgg aaatgcaaac
     4981 agccttcatt ctggtggaat ggtggaggga caggactgcc attatatttc tattactttc
     5041 gatccaaagc tgatctatgg atatgaaaac agtcttattt atgagaaata tgtgatgcct
     5101 gtggtccggg acggagcgct ttgtgccgtt gtttttgatg ggagccagga gtggcatgga
     5161 gagatgattg aattgatacg ggggatcatt tctgcctatg acggacagga agatggatat
     5221 gaaatggaac tgactggaaa gttgttgaaa ttctggcagc ttcttgtgct aaatgtggac
     5281 cggaaaaagg gaatttccgg aaaagaacag aaagattatg aacggatccg tgagatctta
     5341 cgttttatcg aaaaaaatta tgataagaaa ctgaccgttg accagatcgc ggaaaatgta
     5401 tatatgtgta ccagccagtg cagccgttta tttaagaaat atatgaaaaa gacattgttt
     5461 gactttattc tggaatatcg gattgagcgc agtctggcag atctgttgga tcctggctgt
     5521 tcgatggcag aggcagcagc cggagccggt tttaatgact ccaattatta ttcaaaggta
     5581 tttgtgcgga tcaagggatg tgcgccatcg gtgtatcgga agaagatgcg gggaagatag
     5641 ggcataattg cgccagtgca catcctaagc cgcccagagg ggcggcaaaa aggtgtgaaa
     5701 ggcatggtca atacagctct gcgatccgtg tgatccccac atagatatgg gagatcttat
     5761 accaggtggc aaagtcggtg acacctgtct ggggcagacc gaagatattc tggaattccc
     5821 gcactgcagc cgctgtctgc tgtccgtaga tgccgtcagg ctggatgcgg gggattgctg
     5881 tatatacctg ggcaatggca tctaactgct cctgaagttg gcggacctta tcaccggaac
     5941 ttcccactgt caggtcatag cccggccagg aagcagggat cccggcaatc tgttccgctg
     6001 tgttgatata gatgctgtca ccgtagtaat agcgaaggat ctcaataggg ctgtagccct
     6061 gctggcctaa ggagcaggat ccccattgtg tcaaccaaat tgccagtgcg tatagatgcc
     6121 cgcaaacccg cataaatcaa gggtttgcgg gcatttctgg attttcggtc aaataaaaat
     6181 ctacgtcaat gtgactgtta gcccggtcgt aggtgatctt ctctacaatg cttttcaggg
     6241 ctgcattttt ctcctgggga gtgaaggagt cagactgcag gatgtccagg atagaggaga
     6301 tgctctgcag cattttcctg gtattgtctt ccggttcttc tggtacctgg gtgagtttag
     6361 ctagctgttc ttccagttct gttcgttcct ggctgatacg ttctttgtta gcccggtatt
     6421 cttctatggt gtcaattcca tctatgtaag cttcttttgc acgaagttcc tttgagtcca
     6481 gtcgagacaa tttcttttca atatttttaa tttcagcatc tggagcatgg tctgtctgac
     6541 gctggcgcag ggagtaagaa acggatccgt tgttaagggc atccttaaga gcgtttaaaa
     6601 cagcagggcc gatcacactt tcattgacat agcagttagt ggaacatttg ccttttaagt
     6661 atccgtagca ctggaaatag aaagtatccg gtagtgactt tctgtggact acgcaggaag
     6721 aaagggaccg accacagccg gagcatttta gaagccctga gagccaatgc ttggtcactt
     6781 cggaaggctt ggcatttctg ggtgtgtatt ctttattaaa acgctcttga gcctgctca
//
LOCUS       DBMDUA010000152         6760 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4744_length_6760_cov_1.946238, whole genome shotgun sequence.
ACCESSION   DBMDUA010000152 DBMDUA010000000
VERSION     DBMDUA010000152.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4744_length_6760_cov_1.946238"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /gene="miaB"
                     /locus_tag="ACZPCL_10460"
     CDS             <1..1233
                     /gene="miaB"
                     /locus_tag="ACZPCL_10460"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR1162923.1"
                     /translation="TCTVRENANLRVYGRLGQLGARKKKHPHMMIALCGCMMQEKEVV
                     EKIKKTYRFVDLIFGTHNIFKLAELVSLCLEERLEELEARGREDKKPKHKMVVDVWKD
                     TDQIVEDLPVERKYSFKSGVNIMFGCNNFCSYCIVPYVRGRERSRRPGEIIGEIKNLV
                     EDGVVEVMLLGQNVNSYGKGLDEPLNFAELLEEVEKIEGLERIRFMTSHPKDLSDELI
                     EVMAKSKKICRHLHLPLQSGSSRILKAMNRRYTKEQYLALAEKIKTAIPDISLTTDII
                     VGFPGETEEDFQETLDVVRKVRFDSAFTFIYSKRTGTPAAAMENQVPEDVVKNRFDRL
                     LKEVQDISAEVCGRDVHTVQTVLVEEVNDHSPELVTGRMSNNTIVHFPGDASLIGKLV
                     DVYLEESKGFYYMGRLNQ"
     gene            1401..4046
                     /gene="mutS"
                     /locus_tag="ACZPCL_10465"
     CDS             1401..4046
                     /gene="mutS"
                     /locus_tag="ACZPCL_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR1162924.1"
                     /translation="MMQHYMETKKEYPDCILFYRLGDFYEMFFDDALKASKELEITLT
                     GKECGLKERAPMCGVPFHAVDSYLYRLVQKGYKVAIAEQMEDPKLAKGLVKREVIRVV
                     TPGTITSAQALDETKNNYLMGIVYLDSRYGISTADISTGEFLVTEVESDRELFDEINK
                     FSPSEIICNNAFYVSGVDLDELKNRYQVAISALDSHFFSDESCKKVLKEHFKVGSLSG
                     LGLDDYPTGVIAAGAVMEYMYETQKSTLEHITTITPYSTGQYMVIDTSTRRNLELVET
                     MREKQKRGTLLWVLDKTKTAMGARLLRNYIEQPLIHKNEILRRQDAVEELNMNYISRE
                     EICEYLNPVYDLERLIGRISYKTANPRDLLAFKTSLEMLPYIKKLLGEFQSDLLKELE
                     SELDPLEDIYELIGKAIVDDPPITIREGGMIREGYDEEADRLRHAKTEGKTWLGELEN
                     REKEKTGIKNLKVKFNKVFGYYFEVTNSFKDMVPDYFVRKQTLTNAERFTTDELKQLE
                     DVIMGAEEKLVSLEYDLFCQVRDSIAAQVVRIQKTAKAIAGIDVFCSLSSVATRRNYV
                     KPSINEKGVIQIKNGRHPVVEQMMRDDLFVANDTFLDNGKNRLSIITGPNMAGKSTYM
                     RQTALIVLMAQLGSFVPAQEANIGICDRIFTRVGASDDLASGQSTFMVEMTEVANILR
                     NATRNSLLVLDEIGRGTSTFDGLSIAWAVVEHISNTKLLGAKTLFATHYHELTELEGT
                     IAGVKNYCIAVKEQGDDIVFLRKIVRGGADKSYGIQVAKLAGVPDSVISRAKEIASEL
                     TENDITAKAKEIAAVSANVVQHKAVPKPDEVDMQQMSFFDTVKDDDIIKELDGLELST
                     MTPIDAMNTLYRLQTRLKNRWKENL"
     gene            4043..4966
                     /gene="trmD"
                     /locus_tag="ACZPCL_10470"
     CDS             4043..4966
                     /gene="trmD"
                     /locus_tag="ACZPCL_10470"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR1162925.1"
                     /translation="MNFHILTLFPDMVENGLKTSITGRAIESGAISVEAVDIRDYTTD
                     KHRHVDDAPYGGGAGMVIQPAPVCDAYEALCKKLGKRPRVIYMTPQGRVFNQSIAQDL
                     AKEEDLVFLCGHYEGIDERALELIQAEYLSAGDFVLTGGELPSMVMIDCISRLVPGVL
                     GNGDSAEIESFYDNLLEYPQYTRPEVYEGKTVPEVLLSGHHKNIESWRREQSIRRTLE
                     RRPDLLEDASLTLKEQKFLDSLLKEQGESQLKELEQLVREAVKSDETPGSDREYYQQM
                     KKVKKLLNEKKATLQELKGYYKVLGALKQEI"
     gene            4998..6305
                     /locus_tag="ACZPCL_10475"
     CDS             4998..6305
                     /locus_tag="ACZPCL_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR1162926.1"
                     /translation="MDPSGNTIVLRIIIIVILLALSAYFSSAETALTMVNKIRMRTLA
                     DDGNKAAACVLKLHENKSKMLSAILIGNNVVNLSTSSIMTILAADIFGNAAVGIATGV
                     LTILILIFGEITPKTMASLEADKIAMKSAGNIYLLMTILTPVIWAVNKMSGCILRLLH
                     VDPNKKTDVMTEDELRTIVAVGHEKGVIETDEKNMINNVFDLDDSVAGDIMVPRVNMT
                     FIDIDANYEELMEKFRKTRYTRFPVYQDSKDNVVGIVNIKDLLLEDEAENFSIKDHLR
                     KPLYTFEAKKVSELMLEMRKTSNNIAVVLDEYGTTAGLITLEDILEEIVGDIRDEYDA
                     DEEKDFVKLGHGVYLVDGAMSLDDVNDRLGLSLYSEDYDSIGGLMIEKLDHIPVQGEV
                     VTVGNIRLSAHRVNGNRIKKVCIQILKTDGKSGKEEGKNSEKQ"
     gene            6461..>6760
                     /gene="rplS"
                     /locus_tag="ACZPCL_10480"
     CDS             6461..>6760
                     /gene="rplS"
                     /locus_tag="ACZPCL_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR1162927.1"
                     /translation="MNEIIKNIEAAQLKENVPSFNVGDTVKVYNKIKEGNRERIQIFE
                     GTVIKRQNGGARETFTVRKNSNGIGVEKTWPLHSPSVDNVEVVRKGKVRRAKLYYV"
BASE COUNT         2097 a         1303 c         1679 g         1681 t
ORIGIN      
        1 acctgtacag tccgtgaaaa tgcaaatctg cgtgtttacg gaagactggg ccagttaggt
       61 gccagaaaga aaaaacatcc acacatgatg atcgctctct gtggctgcat gatgcaggaa
      121 aaggaggtag tagaaaagat caaaaagact taccgttttg ttgatcttat tttcggaacc
      181 cataacattt tcaagctggc agaattagta agcctgtgtc tggaagagcg tttagaggaa
      241 ctggaagccc ggggccggga agacaagaag ccaaagcaca aaatggtagt ggatgtatgg
      301 aaagatacag accagatcgt agaagacctg ccggtagagc gaaagtattc ctttaaatcc
      361 ggtgtcaata tcatgtttgg ctgcaataat ttctgcagct actgcatcgt accatatgta
      421 cgtggccgtg aaagaagccg cagaccaggt gagatcattg gtgagatcaa aaatctggtg
      481 gaagacggcg tagtagaagt tatgctgtta ggtcagaatg taaactccta cggaaaagga
      541 ctggatgagc ctttaaactt tgctgaactt ctggaagaag tagaaaagat cgagggactg
      601 gagcgcatcc gtttcatgac ctctcatcca aaggatcttt ctgatgaact gatcgaagtg
      661 atggcaaaat caaagaagat ctgccgccat ctccatctgc ctttacagtc cggtagcagc
      721 cgtattttaa aggccatgaa ccgccgttat acaaaggaac agtatctggc actggctgag
      781 aagatcaaaa cagctattcc ggacatttca ttgacaacgg atattattgt aggtttcccg
      841 ggagaaacag aggaagactt tcaggagacc ttagacgtag taagaaaagt acgctttgac
      901 agcgccttta cctttattta ttccaaacgt accggtacac cggcagctgc tatggaaaac
      961 caggtaccgg aagatgtggt aaagaaccgt tttgaccgcc tgttaaagga agttcaggat
     1021 atttctgctg aagtctgtgg aagagatgta cacaccgttc agactgtcct ggtagaggaa
     1081 gtaaacgacc attcaccgga acttgtaact ggaagaatgt caaataatac cattgtacat
     1141 ttccctggag atgcatctct cattggaaag ctggtagatg tatacttaga ggaaagcaag
     1201 ggcttttatt atatgggacg gctgaaccag taatctgatg acccgcccat acctgtattt
     1261 ttacaattat gacagacaaa cggacagata gaaaggttac agtttggcca tgggttggtt
     1321 tgtatgaact gtaacagatg gacagataca catagaagag aggaacacca ttggaaatcg
     1381 attattcaca gctgtctccc atgatgcagc attatatgga gacaaaaaaa gagtacccgg
     1441 actgtatctt attttaccgc ctgggggatt tttacgaaat gttctttgat gacgcactga
     1501 aagcatccaa agaactggag atcacactga caggtaagga atgtggttta aaagaacgtg
     1561 cgcctatgtg cggcgtacct tttcacgcgg tagactccta tctttaccgc ctggtacaaa
     1621 agggctataa agttgctatt gcagagcaga tggaagatcc caagctggca aaaggactgg
     1681 taaaacgaga ggtgatccgt gtggtaacac cgggaaccat taccagtgcc caggctttgg
     1741 acgagacaaa aaataactac ctgatgggca ttgtatatct ggacagccgt tatggtattt
     1801 ccacagcgga tatcagtacc ggtgagttcc tggtaacaga ggtggagtca gaccgggaat
     1861 tatttgacga gatcaataaa ttttctccct ctgagatcat ctgcaacaat gccttttatg
     1921 tatccggtgt agatcttgac gagttaaaga accggtatca ggtggcgatc tctgctctgg
     1981 acagtcattt cttttcagat gagtcctgca aaaaggtatt aaaagaacat tttaaagtag
     2041 gttccttaag cggactggga ctggatgatt atcctaccgg tgtgatcgca gcaggtgctg
     2101 tgatggagta tatgtatgag acccagaaga gtaccctgga gcacattaca accataaccc
     2161 catattctac cggccagtac atggtcattg acacttctac cagaagaaac ctggaattgg
     2221 tggagactat gcgtgaaaag cagaagagag gaactctttt gtgggtgctg gataagacca
     2281 agacagctat gggagccaga cttctgcgca attatattga gcagcctctg atccacaaga
     2341 atgagatcct gcgccgtcag gatgcagtgg aagaactgaa tatgaattat atttccagag
     2401 aagaaatctg cgaatactta aatccggtgt acgatctgga gcgcctcatc ggccgtatca
     2461 gctataaaac agccaatcca agggatcttc tggcatttaa gacttccctg gaaatgcttc
     2521 cttatatcaa aaagctgtta ggagagtttc agtcagatct tttaaaggaa ttagagtcag
     2581 aattagaccc attagaggat atttatgagc tgatcggaaa agccattgta gacgatccgc
     2641 ctattaccat ccgtgagggc ggcatgatcc gagaaggcta tgatgaagag gcagaccgac
     2701 tgcgccatgc aaagacagag ggcaagacct ggcttggtga actggaaaac agggaaaaag
     2761 aaaagaccgg cattaagaat ttaaaggtca aattcaacaa ggtatttggc tattactttg
     2821 aagtcaccaa ctcctttaaa gatatggttc ctgattattt tgtaagaaaa cagaccctga
     2881 ccaatgcaga gcgttttaca acagatgagt taaagcagtt agaagacgtg atcatgggag
     2941 ctgaggaaaa gctggtatct ttggaatacg atctgttctg tcaggtccgt gactccattg
     3001 cagcccaggt agtgcggatc cagaagactg caaaagccat tgcaggtatt gatgtattct
     3061 gctccttatc ttccgttgca accagacgga actatgtaaa accttctatt aatgaaaaag
     3121 gcgtcatcca gatcaaaaac ggccgtcatc cggtggtaga gcagatgatg cgggatgacc
     3181 tgtttgtggc aaatgatact ttcctggata atggaaagaa ccgcctttct attatcaccg
     3241 gccctaacat ggcaggtaag tctacctata tgagacagac ggccctcatc gtgctgatgg
     3301 cccagctggg aagttttgta ccggcccagg aggcaaatat cggtatctgc gaccgtatct
     3361 ttaccagagt aggcgcttct gatgaccttg caagcggcca gagtaccttt atggtggaga
     3421 tgaccgaggt tgccaatatc ctgcgaaatg ccaccagaaa cagcctgtta gtcttagatg
     3481 agatcggccg tggaaccagt acctttgacg gtctttctat tgcatgggcc gtagtagaac
     3541 atatcagcaa tacaaagctg ttaggcgcaa aaaccttatt tgccacccat taccatgaac
     3601 tgacagagtt agagggaacc attgccggag taaagaatta ttgcatcgct gtaaaagaac
     3661 agggagatga tatcgtattc ctgcgcaaga tcgtccgtgg cggcgctgac aagagctatg
     3721 gtatccaggt agcaaagttg gcaggtgttc cggactctgt tatttccaga gcaaaagaga
     3781 tcgccagtga gctgacggaa aatgatatta cagcaaaggc aaaggagatc gccgcagtca
     3841 gcgccaatgt ggtacagcac aaggcagttc caaagccgga tgaggtagat atgcagcaga
     3901 tgtctttctt tgatactgta aaagatgatg atatcatcaa ggagctggac ggactggaac
     3961 tgtctaccat gacaccgatt gatgccatga ataccttata ccgccttcag accaggctta
     4021 agaaccgttg gaaggagaat ttatgaattt ccatatatta acattattcc ctgatatggt
     4081 ggaaaatggt ttaaagacca gtatcaccgg ccgagctatt gaaagcggcg ccatttccgt
     4141 agaggcagtg gatatccggg attatacaac tgacaagcac agacatgtag atgatgcccc
     4201 ttatgggggc ggcgccggaa tggtgatcca gccggcacct gtgtgtgatg catacgaggc
     4261 tctttgtaaa aaactgggaa aacgtccaag agtgatctat atgactcccc agggtcgtgt
     4321 gtttaaccag tctattgccc aggatctggc aaaagaagaa gatctggtgt ttttatgcgg
     4381 acattatgaa ggtattgatg agagagccct ggagctgatc caggcagaat atctgtcagc
     4441 aggtgatttt gtccttacag gaggcgagct tccttctatg gtgatgatcg actgtatttc
     4501 ccgtctggta cctggggtgc tgggaaatgg ggactccgca gagatagagt ccttctatga
     4561 taatctgctg gaatatcccc agtataccag accagaggtt tatgagggga aaacagttcc
     4621 agaagtcctt ctttccggac accacaaaaa tattgagagc tggcgcaggg agcagtccat
     4681 ccgcaggact ctggaaagac ggccggatct gttagaagat gcatccctta cattaaagga
     4741 gcagaagttt ttagacagtc ttttaaaaga gcagggagag agccagttaa aagagttaga
     4801 acagctggta agagaagcag taaaaagcga tgaaactcct ggaagcgacc gggaatatta
     4861 ccagcagatg aagaaagtaa aaaaacttct caatgaaaaa aaggctacct tacaggaact
     4921 gaaaggctat tataaggttc ttggagcgtt aaaacaggaa atataagtta tattaagatt
     4981 taaggagatt gtgagaaatg gacccatcag gcaatacgat tgtcctgcgt atcattatta
     5041 ttgttatttt actggcgtta tctgcgtatt tttcctctgc ggaaacagca cttaccatgg
     5101 taaataagat ccgtatgcgt acccttgctg acgacggaaa caaggcggcg gcctgtgttt
     5161 taaaacttca tgaaaataaa agcaagatgt taagtgccat cctcattgga aataatgtgg
     5221 taaacctgtc cacttcctct attatgacca ttctggcagc tgatattttc ggaaatgcag
     5281 ctgttggtat tgctaccggt gtgcttacga tcctgatcct gatctttggt gagattacac
     5341 caaagaccat ggcttcctta gaggctgaca agatcgcaat gaaaagtgca ggaaacattt
     5401 atcttttaat gacgatcctt acacctgtga tctgggcggt caataagatg tcaggctgca
     5461 tccttcgtct gctccatgtg gatcccaata aaaagacgga tgtcatgaca gaagacgagc
     5521 ttcgtaccat tgtggcagtg ggtcatgaaa agggagttat cgagacagat gaaaagaata
     5581 tgatcaataa tgtgtttgat ctggatgatt ctgttgcagg ggatattatg gttccccgtg
     5641 taaacatgac ttttattgat atagatgcca attacgaaga actgatggag aaattccgta
     5701 agacccggta tacacgtttt ccggtctacc aggacagcaa ggataatgtg gtaggtattg
     5761 taaatattaa agatcttttg cttgaagatg aggcagaaaa cttttctatt aaagatcatt
     5821 taagaaagcc gctgtatacc tttgaagcta aaaaagtttc agagctgatg ctggaaatgc
     5881 ggaaaacttc caataatatt gcagtagttt tagatgaata cggaaccaca gcaggcctga
     5941 tcacattaga agacatcttg gaagagatcg taggcgatat ccgtgatgaa tatgatgctg
     6001 acgaggaaaa ggattttgta aaactgggtc acggcgttta tctggtagac ggtgccatga
     6061 gcctggatga tgtaaatgac agactgggac tttccctgta ttctgaggat tacgactcca
     6121 ttggcggtct tatgatagaa aaattagacc atatcccggt ccagggagaa gtagtgacag
     6181 taggaaatat ccgtctgtcc gcccacaggg tcaatggaaa ccgtattaag aaggtctgta
     6241 tccagatttt aaagacagat ggaaaatccg ggaaagaaga aggaaaaaac agcgaaaaac
     6301 agtaaaaaaa cgtagaaaag tgcttgcatt tagcaggaaa ctatgataat atcaattcgt
     6361 tgtgacaaga atagatggtc ctctgctact gaagtattat gaacaggatt ttatgataca
     6421 gagtattaag aacatccaat agatgaagga ggttcacacc atgaacgaaa ttattaagaa
     6481 tatcgaagca gcacagttaa aagagaacgt acctagcttt aatgttggtg atactgtaaa
     6541 ggtatacaac aagatcaaag agggtaaccg cgaaagaatt cagatctttg aaggaactgt
     6601 tatcaagaga cagaacggcg gagctagaga gactttcaca gtaagaaaga actctaacgg
     6661 tatcggcgtt gagaagacct ggccattaca ttccccaagc gttgacaacg tagaagttgt
     6721 tcgtaagggt aaagtaagaa gagcaaaact gtactacgta
//
LOCUS       DBMDUA010000153         6682 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4800_length_6682_cov_2.881781, whole genome shotgun sequence.
ACCESSION   DBMDUA010000153 DBMDUA010000000
VERSION     DBMDUA010000153.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6682)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4800_length_6682_cov_2.881781"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(296..1132)
                     /locus_tag="ACZPCL_10485"
     CDS             complement(296..1132)
                     /locus_tag="ACZPCL_10485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR1162928.1"
                     /translation="MKLNEIEITDFNAPELDIYARCNEAQLLHYFEPNPGIFIAESPK
                     VIERALNAGYVPISFLAEKRLVSADGGETRQLLEKYPDVPVFTAEYNVLTKLTGFALT
                     RGMLCAMKRNVLPSVEDICKHANRIAILEDVVNPTNVGAIIRSAAALGMDAVLLTHGC
                     ADPLYRRASRVSMGTVFQIPWTILPKNSWPDASMDTLKALGFKTAAMALKTDSVSIDD
                     PVLTSHEKLAIVLGTEGEGLKDATIAACDYTVLIPMFHGVDSLNVAAASAVAFWQLGR
                     RK"
     gene            1334..2965
                     /gene="hcp"
                     /locus_tag="ACZPCL_10490"
                     /gene_synonym="priS"
     CDS             1334..2965
                     /gene="hcp"
                     /locus_tag="ACZPCL_10490"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HIR1162929.1"
                     /translation="MENKMFCYQCQETANCTGCTLTGVCGKNPQVAAAQDLLVYVTKG
                     YCEVTTRLRKEGTNISDAENRMVVMNLFMTITNANFDKEAIENRIHDTLHVKKELLKK
                     VKDTSGLSEAALWDGSGDWEVIAGKVGVLSNPDEDIRSLQQLITYGIKGLCAYTKHAN
                     ALMHTDPKLDAFIQETLAKTLDNSLSAEELVALTLKTGEYGVKGMAMLDAANTGAYGD
                     PEITAVNIGVRKNPGILVSGHDLRDLEMLLEQTKGTGVDVYTHSEMLPACSYPAFKKY
                     PHFVGNYGNAWWQQKEEFESFNGPILMTTNCIVPPKASYKDRLYTTGAAGYPGCTYIP
                     GGLGEKKDFSQIIEQAKKCPPPKELEFGTIPGGFAHAQVIALADKVVAAVKSGKIKKF
                     VVMAGCDGRMKSREYYTEFAKALPKDTVILTAGCAKYRYNKLKLGDIDGIPRILDAGQ
                     CNDSYSLAVIAMKLKEVFGLDDINDLPIIYNIAWYEQKAVIVFLALLSLGIKNIHLGP
                     TLPAFLSPAVLKVLVEKFHIAGITTVEEDMKLFFG"
     gene            3046..3351
                     /locus_tag="ACZPCL_10495"
     CDS             3046..3351
                     /locus_tag="ACZPCL_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162930.1"
                     /translation="MKVIKEKPRRRQYPFPNITESRCRWKSGISRRKEKITPELPAEV
                     LSRPGRISCVKGTHMMVCSNRWLNESKLSSFYGDGSFFFFMVMHRQVTDPCIENSGR"
     gene            3408..6536
                     /gene="ileS"
                     /locus_tag="ACZPCL_10500"
     CDS             3408..6536
                     /gene="ileS"
                     /locus_tag="ACZPCL_10500"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR1162931.1"
                     /translation="MYDKVPTDLKFVEREKEVEKFWEDEHIFEKSIKMREGCQPYVFY
                     DGPPTANGKPHIGHVETRVIKDMIPRFRAMKGYMVPRKAGWDTHGLPVELEVEKKLGL
                     DGKDQIEKYGLEPFIKQCKESVWKYKGMWEDFSGTVGFWADMDNPYVTYHNSFIESEW
                     WALKQIWDKGLLYKGFKVVPYCPRCGTPLSSHEVAQGYKDVKERSAVVRFKVKGEDAY
                     ILAWTTTPWTLPSNLALCVNPNEEYVKVKAADGYTYYIAHALMEKVLGEGCEVLETYV
                     GKDLEFKEYEPLYDCSVPYCEKQHKKAFYVVCDTYVTLTDGTGIVHIAPAFGEDDANV
                     GRKYNLPFVQLVDAKGDMSADTPFAGKFVKDADPLVLKDLKEKGLLFDAPAFEHSYPH
                     CWRCGTPLIYYARESWFIKMTAVKDDLIRNNNTINWVPESIGKGRFGDWLENIQDWGI
                     SRNRYWGTPLNIWECECGHQHSIGSIEELKSMSDNCPDDIELHRPYIDAVTIRCPKCG
                     KQMKRVPEVIDCWFDSGAMPFAQHHYPFENKELFESQFPAQFISEAVDQTRGWFYSLL
                     AESTLLFNKAPYENVVVMGLVLDENGQKMSKSKGNAVDPFDTLQKHGADAIRWFFYTC
                     SAPWIPKRYQDKAVTEGQRKFMGTLWNTYAFWVLYANIDNFDATKYSLEYDKLPVMDK
                     WLLSKMNSMVKAVDDNLMNYRIPEAAKALQEFVDDMSNWYVRRSRERFWAKGMEQDKI
                     NAYMTLYTALVTVAKAAAPMIPFMTEQIYQNLVRKIDKTAPESVHLCDFPVVKEEWID
                     EKLEADMEEVLDTVVMGRAARNTANIKNRQPIAQMYVKADHALSDFYVEIIEEELNVK
                     KVTFTDDVRAFTSYTFKPQLKTVGPKYGKLLGKIREALTNLDGSAAMDTLNETGALTF
                     DFDGQEVVLTKDDLLIDVAKKDGYVTEEDNYVTVVLDTNLTPELIEEGFVREVISKIQ
                     TMRKEAGFEVMDHIGVYQSGNDKIAELIKNNADEIKSEVMADYIHLGEMKGFTKEWNI
                     NGETVMLGVEKL"
BASE COUNT         1984 a         1328 c         1672 g         1698 t
ORIGIN      
        1 tacggtagaa catatcgaag atgacagttt catttgcgtc tttttcctta aaatagccct
       61 gcatcatcgt cagttcatac aggtcagtga gtagtgtgag atttctgtga tccataagtt
      121 ggctccctcc tggtacacag tctttggttt gaaataaatg gctgtgtgcg gttgtttgta
      181 aagtttccgt aacaatatag catatttatg taaaaaaagg aacatatcgt tacaaattta
      241 acaatattat atacacatga caagacaagt gttctgacag agcgaaataa aatgcctatt
      301 tcctccgtcc aagctgccag aaagccacag cactggctgc tgccacattc agggagtcta
      361 ctccgtggaa cataggaatg agaacggtgt agtcgcaggc tgcaatagta gcatctttca
      421 ggccttcgcc ttctgtgcca aggacaatag ccagcttttc atgggaagtt aaaacaggat
      481 cgtcaatgga tacagagtcg gtttttaatg ccatagcagc agttttaaag cccaaagctt
      541 ttaaggtatc catggaagca tccggccaag aatttttggg aagaatggtc catgggatct
      601 ggaatacagt tcccatgctc acacgggagg cgcggcggta taatggatca gcgcagccat
      661 gggtgagaag tacagcgtcc atgccaaggg cagcagcaga acggataatg gctcctacat
      721 tagttgggtt tactacatct tctaaaatag caatgcggtt tgcatgttta catatatctt
      781 ctacagaagg aaggacattg cgcttcatgg cgcagagcat gccacgggtc agtgcaaagc
      841 cggtgagttt tgtaagtaca ttgtattcag cggtaaatac aggcacatca gggtattttt
      901 caagaagctg tctggtttcg cctccgtcag ctgagaccag ccgtttctct gccagaaaag
      961 aaatgggaac atatcctgca tttaatgccc gttcaatcac cttggggctt tcagcaataa
     1021 aaatgccggg attcggttca aaatagtgga gaagctgggc ctcgttgcag cgggcgtaga
     1081 tgtcaagctc cggggcgtta aagtctgtta tttcaatttc atttaatttc ataaaaataa
     1141 gtcctttaat gggtgatatg tagtttagtg atatatcatt tccgcgatcg cgggaaaata
     1201 tatctgtttt ctagtataga ggaaatgtat attcttggca accggacata gttacatggt
     1261 ttcaggaaaa atggttattc taaatctatc aaagaattat caaaaattta ttagtgcaaa
     1321 ggagaccaga tccatggaaa ataaaatgtt ctgttaccag tgtcaggaaa cagccaactg
     1381 cactggctgt actttaaccg gcgtctgtgg caaaaatccc caggttgcgg cagctcagga
     1441 tcttttggtg tatgtgacaa aggggtattg tgaggtaaca accagactgc gtaaggaagg
     1501 cacgaatatc agtgatgctg aaaaccgcat ggtggtaatg aacctgttta tgaccattac
     1561 aaatgctaat tttgataaag aggccattga gaaccggatc catgacactc ttcatgtgaa
     1621 aaaagaatta ttaaagaagg taaaagatac ctccgggctt tctgaggctg ctttgtggga
     1681 cggaagcggg gactgggagg tgatcgccgg aaaagtggga gttctttcca atccagatga
     1741 ggatatccga agccttcagc agttgatcac ctatggcatc aagggacttt gcgcttatac
     1801 caaacatgcc aatgccctga tgcatacaga tccaaagctg gatgcgttta tacaggaaac
     1861 tctggcaaag acattggata acagcttgtc ggcagaggaa ttagtggcgt tgaccttaaa
     1921 aacaggagaa tacggtgtaa agggaatggc catgttagat gctgccaata ccggggctta
     1981 tggagatcca gagatcacag cagtgaatat cggtgtaaga aaaaatccgg gcattctggt
     2041 ttctggtcat gacctgcggg acctggaaat gcttttggag cagacaaaag gaacaggcgt
     2101 ggatgtgtat acccattcag aaatgcttcc tgcctgcagt tatccggcct ttaagaaata
     2161 tccccatttt gtggggaatt atggcaatgc ctggtggcag cagaaagaag aatttgagtc
     2221 atttaacggg ccgatcctta tgaccactaa ctgtatcgtg ccgccaaaag ccagttataa
     2281 agaccgtctt tacaccaccg gtgcagcagg atatccgggc tgtacctata ttccaggggg
     2341 cttaggagaa aagaaggatt tttcccagat catagaacag gcaaagaaat gtccgccgcc
     2401 taaagaactg gaatttggta cgatcccagg tggctttgcc catgcccagg tgatcgcgct
     2461 ggcagataaa gtagtggcag cggtaaagtc cgggaaaata aagaaattcg tagtcatggc
     2521 gggctgtgac ggacgcatga agagccgtga atattataca gaatttgcca aagcgctgcc
     2581 aaaagatacc gtgatcctca cagcaggctg cgccaagtac cgctacaata aattaaaact
     2641 gggagacatt gacggcatcc caaggatcct ggatgcaggc cagtgtaacg actcctattc
     2701 cctggcagtg atcgccatga agctgaaaga ggtatttgga ctggatgata tcaacgacct
     2761 tcctattatt tataacattg cctggtatga gcagaaagcc gtgatcgttt tcctggccct
     2821 tctgtcttta ggtatcaaaa acatccactt aggtccaacg ctgcctgcgt tcctgtcccc
     2881 ggcggtgttg aaagtgctgg tggagaagtt tcatattgcg ggaatcacga cggtggaaga
     2941 ggatatgaag ttgttttttg gatagatact gtgtattttt aaagttttct cctcagttta
     3001 gccgttgaca tttccatccc cagtcattat aatgtaactg ttataatgaa agttataaaa
     3061 gaaaagccaa gaaggagaca gtatcctttt ccaaacatta cagagagccg gtgcaggtgg
     3121 aaatccggta tcagtaggag aaaggagaag atcactccgg agctgccagc tgaagtttta
     3181 agtaggccgg gccgcatttc ctgcgttaag ggaactcata tgatggtatg cagtaacagg
     3241 tggttgaacg aatctaagct ttcgtccttt tacggggatg gaagcttttt cttttttatg
     3301 gtgatgcacc gtcaggtgac agatccatgt attgaaaatt cagggcgctg accttgaaaa
     3361 gtacctgtga gacattcttc aaatttagaa atggaggaaa agcaaggatg tatgacaaag
     3421 tccccacaga ccttaagttt gtggaaagag aaaaagaagt cgaaaagttc tgggaagatg
     3481 aacacatttt tgagaagagt attaagatgc gtgaaggctg ccagccatat gtattctatg
     3541 atggaccgcc aaccgcaaac ggtaagccgc atatcggcca tgttgagacc cgtgtaatca
     3601 aggatatgat cccgagattt cgcgctatga agggctatat ggttccaaga aaggccggct
     3661 gggatactca cggacttcct gtagaactgg aagtagaaaa gaagttaggc cttgacggaa
     3721 aggaccagat cgaaaaatat ggtctggagc catttatcaa gcagtgtaag gaaagtgtct
     3781 ggaaatacaa gggcatgtgg gaagatttct ccggcactgt tggtttctgg gctgatatgg
     3841 acaatccata tgttacctac cacaattcct ttattgagtc tgaatggtgg gcattaaagc
     3901 agatctggga taaaggcctg ttatacaagg gctttaaggt agttccttat tgcccacgct
     3961 gcggtacccc gctttcctcc cacgaggtag cgcagggata caaggacgta aaagagcgtt
     4021 ctgcagtggt acgttttaaa gtaaaagggg aagatgctta tatcctggca tggaccacaa
     4081 ccccatggac ccttccaagc aacctggcac tttgcgtaaa cccaaatgaa gaatacgtaa
     4141 aagtgaaggc agcagacggc tatacctatt acattgcaca tgcattaatg gaaaaggtat
     4201 taggagaagg ctgtgaggtt ctggaaactt atgtaggtaa ggatctggaa ttcaaggaat
     4261 atgagccatt atacgactgc tccgttccat actgcgagaa gcagcacaaa aaagcgttct
     4321 atgttgtatg tgatacctat gtaaccttaa cagatggtac cggaatcgta cacattgctc
     4381 ctgcatttgg tgaagacgat gctaatgtag gccgcaagta caacctgcct ttcgtccagt
     4441 tagttgatgc aaagggtgat atgtcagcag atactccatt tgcaggcaag tttgtaaaag
     4501 atgctgatcc attagtatta aaagacttaa aagaaaaagg cctgttattt gatgctcctg
     4561 catttgagca cagctatcca cactgctgga gatgcggtac tccactgatc tactatgcaa
     4621 gagaatcctg gttcattaag atgactgctg ttaaggatga cctgatccgc aataacaaca
     4681 ccattaactg ggtacctgag tctattggta aaggccgttt cggtgactgg ctggagaaca
     4741 ttcaggactg gggcatttcc agaaacagat attggggaac tccattaaat atctgggagt
     4801 gcgagtgcgg acatcagcat tccatcggaa gcattgaaga attaaagagc atgtctgaca
     4861 actgcccgga tgatattgaa cttcaccgtc catacattga cgctgttacc atccgctgcc
     4921 cgaagtgcgg caagcagatg aagcgtgtac cggaagttat cgactgctgg ttcgactctg
     4981 gtgcaatgcc atttgcacag catcattatc catttgagaa taaggaactg tttgagtccc
     5041 agttcccggc tcagttcatt tccgaggctg tagatcagac tcgtggatgg ttttactccc
     5101 tgttagctga gtctacctta ctgttcaaca aggctcctta cgaaaatgta gttgtaatgg
     5161 gtctggttct ggatgagaac ggacagaaga tgagtaagtc caagggaaat gcagttgacc
     5221 catttgacac cttacagaag catggcgcag atgccatcag atggttcttc tatacctgct
     5281 ccgctccatg gatcccgaag agataccagg ataaggcagt gaccgaagga cagagaaagt
     5341 tcatgggtac cttatggaat acctacgcat tctgggtatt gtatgcaaat atcgacaact
     5401 ttgacgctac caaatacagc ttagaatatg ataagctgcc ggtaatggat aagtggctgc
     5461 tgtccaagat gaactccatg gtcaaggcag tagatgacaa cctgatgaac taccgtatcc
     5521 ctgaggctgc aaaggctctc caggaattcg tagatgatat gagtaactgg tacgtgcgca
     5581 gaagccgtga gcgtttctgg gcaaagggca tggagcagga taagatcaat gcctatatga
     5641 ccctgtacac cgctcttgta actgttgcaa aggcagctgc tcctatgatc ccgttcatga
     5701 cagagcagat ctaccagaac ctggtaagaa agatcgacaa gaccgctccg gaaagcgttc
     5761 atctgtgcga cttccctgta gtaaaggaag agtggattga tgagaagtta gaagcagata
     5821 tggaagaagt tctggatact gttgttatgg gccgtgcagc cagaaataca gcaaatatca
     5881 agaaccgtca gccaatcgcc cagatgtatg taaaggcaga ccatgcatta tctgatttct
     5941 atgtagagat cattgaagaa gagttaaatg taaagaaagt aacctttaca gatgacgtaa
     6001 gagccttcac ttcctataca tttaagccac agttaaagac cgtaggacca aaatacggca
     6061 agctgttagg aaagatccgt gaagcactga ccaacttaga cggaagtgct gctatggata
     6121 ctttaaatga gaccggcgca ttaacctttg actttgacgg acaggaagtt gttcttacaa
     6181 aggatgacct gttgatcgat gtagcaaaga aagatggtta tgtaacagaa gaagacaact
     6241 atgtaactgt tgtcctggat accaacctga ctcctgaact gatcgaagaa ggttttgtcc
     6301 gcgaagtgat cagcaagatc cagaccatgc gtaaggaagc tggctttgag gttatggacc
     6361 acatcggcgt ataccagagc ggcaacgaca agatcgctga gctgatcaag aacaacgcag
     6421 atgagatcaa gagcgaggtt atggctgatt acatccactt aggtgagatg aagggcttca
     6481 ccaaagagtg gaacatcaac ggcgagactg ttatgttagg tgtggaaaag ctgtaaagta
     6541 gccatataat aatggtaaaa tatggtttaa tatccgtatt taagttgaaa tagactggaa
     6601 aaaatggtaa tatgagggac agggcagaaa tgttacctgt cccttgtttg tatcatttat
     6661 ttgtcaaaca gatcagcagc gc
//
LOCUS       DBMDUA010000154         6508 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4916_length_6508_cov_2.586858, whole genome shotgun sequence.
ACCESSION   DBMDUA010000154 DBMDUA010000000
VERSION     DBMDUA010000154.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4916_length_6508_cov_2.586858"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="ACZPCL_10505"
     CDS             complement(<1..920)
                     /locus_tag="ACZPCL_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR1162932.1"
                     /translation="MDKHILRLILKILLPASGWFLLCFFGPRCLKFFMPFVIGWLISM
                     LANPLVRFLERKLCLVRKHSSMVIVAGALALVIGLIYLAVSNGARLLWSFMKDLPDLY
                     AGIEAEVQESLTHLENLFAYAPAGIRTGWEKLGNNLGELVGKLAGSIAPPTVEAAGAV
                     AKMIPSLMVYSVVTILSAYFFIADRDRILAFVRSHMPDWAKKYSQYIRTEARRLIECY
                     FTAQFKIMAVVWIILTVGFFILGVKYSLLWGFLIAFLDFLPVFGAGTALFPWGIIKLL
                     GGEYAFAAGLLLIYVLTQVIRQVVQPKLVG"
     gene            complement(936..1997)
                     /gene="hisC"
                     /locus_tag="ACZPCL_10510"
     CDS             complement(936..1997)
                     /gene="hisC"
                     /locus_tag="ACZPCL_10510"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR1162933.1"
                     /translation="MRPWEKNIRQVVPYVPGEQPAGDKIVKLNTNENPYPPAPGVKKV
                     LEEMEADRLRKYPDPTASILVKELASYYGLNEDQVFVGVGSDDVLAMSFLTFFNSDKP
                     VLFPDVTYSFYKVWADLFKVPFETPVLKEDFTIDVKDYEKENGGVIFPNPNAPTGVYM
                     PLDQVEEILKANQDVVVIVDEAYVDFAGPSALELLDKYENLLVVQTFSKSRSMAGMRI
                     GFAMGHPDLIRALNDVKYSYNSYTMNLPSLLAGVEAVKDKEYFEKTIEKIVATRKHAK
                     KRLTELGFTFPNSQSNFIFASHKSVPAEKIFEALKKEQIYVRYFKTPGLDNSLRISIG
                     TDEEMDTLFRFLEAYLPNA"
     gene            2184..2843
                     /gene="sdaAB"
                     /locus_tag="ACZPCL_10515"
     CDS             2184..2843
                     /gene="sdaAB"
                     /locus_tag="ACZPCL_10515"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HIR1162934.1"
                     /translation="MNVFDILGPVMIGPSSSHTAGAARIGRITRTLLGAPAVKADILL
                     HGSFAKTYKGHGTDKALIAGIMGMATDDVRIRQAPELAKEAGLEVHISTGDIDGAHPN
                     TAKVTLTDANGRQVSLLGSSIGGGNILVTEVNGMEVSLTGQYTTLIVLHKDAPGTIAA
                     VTEVMAEEGINICNFRLSRQKKGGEAVMTIELDGKPSPEINKKINTLPNIYSSTMLQP
                     I"
     gene            2883..3815
                     /gene="sdaAA"
                     /locus_tag="ACZPCL_10520"
     CDS             2883..3815
                     /gene="sdaAA"
                     /locus_tag="ACZPCL_10520"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIR1162935.1"
                     /translation="MNTDFNYSSVASLIQAAEKNNLPVSALVLSQQAQQIELDEKTVY
                     EKMASNFQVMKECIEPGCDEHLKSTSGLTGGDAFKLRRYSESGKSLTGSFLSGALYRA
                     LAVSELNASMGRIVAAPTAGSCGILPAALITMQEEKGCSDHDCVMALFTASAVGMVIA
                     NNASLAGAQGGCQAECGSAAAMAAAAITELAGGTPHMVSQAVAIALKNILGLVCDPVA
                     GLVEIPCIKRNASGVAGAFVAAEMALAGIDSAIPADEVIWSMKRIGDVMSPTLKETAE
                     GGLAATPTGRKLHDQVFGPGNVSGGCSGCSGCHS"
     gene            complement(3859..6324)
                     /locus_tag="ACZPCL_10525"
     CDS             complement(3859..6324)
                     /locus_tag="ACZPCL_10525"
                     /inference="COORDINATES: protein motif:HMM:NF039456.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6449 domain-containing protein"
                     /protein_id="HIR1162936.1"
                     /translation="MTSASLFFKLQKEDLKRRIWVIALLFLGFFFAYPVNLALIMENA
                     ANSQFAMYNGYTPLVDTGTPEYLAKVLEYKTKAVVDLVSYGNVMPLFLMVTAAVVIGA
                     AGFVYLHNQKKVDFYHSLPVRREMLYLVYHVDGILILAVTYLIHLLVLTAAAAAYGVS
                     PAKFAGPMLFGFFMNLLYYMVTYETVIVAMMMTGKIIVGLLATVVFFSFFPVVGALIE
                     GFEDIFFITANQVPNEALFNTLGHLSPVGAYIMSLADISAGKTVEADQILGLLIAICA
                     GAILGLELYRKRPMEAAGKAMAFKKTMAPIRILIVLAAGMGTSMFFWTLQSRLRWGLF
                     GMVVGILLAHCIIEIIYQADFKKLFSHKIQLMGCVAAGVLFFLSFRYDWYGYDRFIPE
                     EGKIASAGLELSIDENFLNWYAHAVEEDGKWVVKHTSNIDFVQNHMQLTDMDTVLTIA
                     EAGVTQAAQERKTRFDQFYGISVARTSGLVVQETAAANAVSVIGGADGPTSIFVAGKV
                     GSGESDPLEKDITISVNVFYNLKNGKQVGRCYNVSLNSIMSAYDTLYASEEYKKGLYP
                     VFEENTGELSKVVYKEAGSIWYQTQDSAVAEEVLKAYQADLLTQTVADRRQEDPVGSL
                     VFIDNNMAAFLQQQGYWKEVMEMPVNVMAGGFESGYYYDGDYTYDIADDYCVSWPVYP
                     SFKNTMEVLKKQSVVPGSYMTAKNIQSIRIDLQSLYVGEEGAIRLPQGDELEKLKEEN
                     PHYSEQGRLDITDSEEIAELIGACQESSLAENNGLCSSFNGDEDLFVYVTMKNGSQIR
                     SNLDLEKLTSRGKELFTGIPVAF"
     gene            complement(6299..>6508)
                     /locus_tag="ACZPCL_10530"
     CDS             complement(6299..>6508)
                     /locus_tag="ACZPCL_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1162937.1"
                     /translation="ADDLKGLQILSQEGRGRLLTLTVRGTKQEAEDIMNRYEPVFFEC
                     IPLSLEEIFISETEVAGYDIRKLIF"
BASE COUNT         1802 a         1596 c         1322 g         1788 t
ORIGIN      
        1 tctcctacta acttaggctg tactacctgg cggatcacct gggtaagtac ataaataagc
       61 agaagccctg ctgcaaaggc atattcccct cccagcagct taatgatccc ccatggaaac
      121 agagctgttc ctgctccaaa taccggaaga aaatctaaaa atgcaatgag aaatccccat
      181 aacaggctat attttactcc aagtatgaaa aatcccactg tcagtattat ccataccact
      241 gccataattt tgaactgggc agtaaaatag cattcgatca gacgtcgggc ctctgtcctt
      301 atatactggc tgtatttttt tgcccagtca ggcatatggc ttcttacaaa agccaggatc
      361 cggtctctgt ctgcaataaa aaaataagct gacagaatag taacaacaga gtacaccata
      421 agcgagggga tcattttcgc cactgcccct gctgcctcta ctgtaggcgg ggcaatggaa
      481 cctgccagct ttcctaccag ttcccccaga ttatttccca gtttttccca gcctgtcctg
      541 atgcctgcag gtgcataggc aaacagattt tccaaatggg tcaggctttc ctgaacttcc
      601 gcttcgatcc ctgcatataa gtcaggcagg tctttcataa aagaccacaa aagcctggca
      661 ccatttgaca cagccagata aataaggcca atgaccagag ccagggctcc ggctacaatg
      721 accatggaac tgtgttttcg caccaggcat agctttctct caagaaaacg caccagcgga
      781 ttggccagca tggagatcag ccagccgatc acaaagggca taaaaaattt caaacacctg
      841 ggtccgaaaa agcataaaag aaaccatccg gacgcaggca gcaggatctt tagtatcaga
      901 cgcaggatat gtttatccat tcactcatct tcctttcagg cattgggtaa atatgcttcc
      961 aaaaagcgga ataatgtatc catttcttca tctgttccta tagaaatacg aagactgttg
     1021 tccagaccag gtgttttaaa atagcgcaca tagatctgtt ctttcttcaa tgcctcaaag
     1081 atcttttctg ccggaacgga cttatgagaa gcgaaaataa aatttgactg ggaattgggg
     1141 aaggtaaatc ccagttctgt aagacgcttc tttgcatgtt tcctggtagc tacgatcttt
     1201 tcaattgtct tttcaaaata ttccttatcc tttactgctt ccacacctgc taagagagat
     1261 ggaaggttca tggtatagga attataagag tattttacat catttaaagc gcggatcaga
     1321 tccggatgtc ccattgcaaa accaatacgc atacctgcca tggagcggga cttgctgaaa
     1381 gtctggacta ctaaaagatt ttcatattta tccagaagtt ccagtgcgga aggccctgca
     1441 aaatccacat aagcctcatc tacgatcact accacatcct gatttgcctt taagatctct
     1501 tctacctgat ccagtggcat atatacacct gttggcgcat ttggatttgg gaagatcaca
     1561 ccgccgtttt ccttttcata atcttttaca tcaatggtaa aatcttcttt taaaacaggt
     1621 gtctcaaacg gtactttaaa aagatccgcc catactttat agaaagaata ggtcacatcc
     1681 gggaaaagca ccggtttatc tgagttgaag aaagtaagaa atgacatggc aagtacatca
     1741 tctgaaccta ctcccacgaa aacctgatcc tcgtttaatc cataataaga agccagttcc
     1801 ttaactaaaa tggacgctgt aggatccgga tacttacgta aacggtctgc ttccatctct
     1861 tctaagacct tttttacacc tggcgccggt ggatatggat tttcatttgt attcaattta
     1921 acgatcttat cacctgcagg ctgttctccc ggtacataag gaactacctg acggatgttt
     1981 ttttcccatg gtctcataag cttagctctc ctctcacatt tgcttctctt gattttatcc
     2041 ttgatacccc cgattgtcaa tatagaagaa gcgttgtttt ttcttacatt ttaatgtata
     2101 ctttcagtaa tgattttatc tcagatcgca aaaaagcttt cttaaaaaat gttttaaaaa
     2161 tctaaatcaa agaaggtgta cttatgaacg tatttgacat acttggtcct gttatgatcg
     2221 gtccttccag ctcccatact gccggcgctg ccaggatcgg ccgtattaca aggacactcc
     2281 ttggagctcc tgctgtaaaa gcagatatcc tccttcacgg ttcctttgca aaaacctaca
     2341 aaggccatgg tacagacaag gcactgatcg ccggaatcat gggaatggct actgatgatg
     2401 tgcgtatccg acaggctccg gaactggcaa aagaagccgg cctggaagta cacatttcca
     2461 caggtgacat tgacggcgcc catccaaata ctgcaaaagt tacattgaca gatgccaacg
     2521 gacggcaggt ttctcttctg ggttccagta tcggaggcgg aaatatcctg gtaacggaag
     2581 tcaatggtat ggaagtttcc cttaccggac agtatacgac tctgatcgtc cttcacaaag
     2641 atgctcccgg caccattgcc gctgttacag aagtcatggc agaagaaggc atcaacatct
     2701 gcaatttccg tctttccaga cagaaaaaag gcggcgaggc ggttatgacc attgaactgg
     2761 acggaaaacc aagtcctgag atcaataaga agatcaatac gcttcccaat atctattcca
     2821 gcactatgct gcagcctatc tgataaagag aacattaaat cttaaggagg tttctttaca
     2881 tcatgaatac tgattttaac tactcttctg ttgcctccct gatccaggct gcagaaaaaa
     2941 ataatcttcc cgtttcagcc ctggtgctga gccagcaggc acagcagata gaactggacg
     3001 aaaagaccgt atatgaaaaa atggcttcta atttccaggt tatgaaggaa tgtatcgaac
     3061 ctggctgtga tgaacactta aaaagtacca gtggacttac aggaggcgat gcctttaagc
     3121 tgcgccggta cagtgaaagc ggcaaaagcc ttaccggttc cttcctttcc ggtgctctct
     3181 accgggcact ggctgtctct gaattaaacg cttccatggg ccgtatcgta gctgctccta
     3241 ctgccggaag ctgcggcatc cttccagctg ccctgatcac catgcaggaa gagaaaggat
     3301 gttctgacca cgactgcgta atggcacttt ttacagcctc tgccgttggt atggtcattg
     3361 caaacaatgc ctctttagca ggcgcccagg gcggatgtca ggctgagtgc ggttctgctg
     3421 ctgccatggc tgctgctgct atcacagagc tggctggcgg aactccccat atggtctccc
     3481 aggcagttgc cattgcctta aaaaatatcc ttggccttgt atgtgacccg gtagcaggtc
     3541 tggtggagat cccctgtatc aagcgcaatg cttccggtgt agccggtgcc tttgttgctg
     3601 cggaaatggc tcttgcaggc attgacagtg cgatcccggc tgacgaggtg atctggtcca
     3661 tgaagcggat cggcgatgtt atgtcaccta ccttaaaaga aacagcagaa ggcggattag
     3721 ccgccacacc aacaggaaga aagcttcacg accaggtatt cggtcctggc aacgtttccg
     3781 gcggatgcag cggctgttcc ggctgtcatt cataaatttt catggttatc tggctataga
     3841 ccggataact gcattttatt aaaaagctac aggaattcct gtaaataact cttttcccct
     3901 ggaagtcagt ttttcaaggt ctaagttgct cctgatctgg cttccatttt tcattgtcac
     3961 gtagacaaac agatcttcat ctccattaaa actgctgcaa agaccattat tctctgctaa
     4021 agagctttcc tggcaggcgc cgatcagctc tgcgatctcc tcagaatctg taatatccag
     4081 acgtccctgt tcagaataat gaggattttc ttcttttagc ttttctaatt catctccctg
     4141 aggaagtctt atggcacctt cttcacctac atataggctt tgcaggtcga tccttatgct
     4201 ctggatattt tttgccgtca tataacttcc cggcacaacg gactgcttct ttaaaacttc
     4261 cattgtattc ttaaaagaag gataaactgg ccaggaaaca cagtaatcat ctgctatatc
     4321 ataagtataa tcaccgtcat aataatatcc ggactcaaac ccgcctgcca taacatttac
     4381 aggcatctcc ataacctctt tccagtagcc ctgctgctgt aagaaagcgg ccatattgtt
     4441 atctataaac accaggcttc ctaccggatc ttcctgtctt ctgtctgcca cagtctgagt
     4501 cagaaggtct gcctggtatg cctttaacac ctcttctgct actgcgctgt cctgtgtctg
     4561 ataccagata cttcctgctt ctttgtagac tactttggaa agttctcctg tgttctcttc
     4621 aaaaacagga tacagaccct ttttgtattc ttctgaagca tacagagtat cataggcact
     4681 cataatgctg tttaaagata cattataaca tcttcccacc tgtttaccgt tttttaagtt
     4741 gtaaaataca ttcacagaaa tcgtaatatc tttctctaaa ggatcacttt caccggaacc
     4801 cacttttccg gcaacaaaga tggatgtagg accgtcagcc ccgccgatca cagaaaccgc
     4861 atttgctgcg gcagtttcct ggaccacaag acctgatgtt cttgccacgg atattccata
     4921 aaactggtca aaacgagttt tccgttcctg ggcagcctgg gttacgcctg cttctgctat
     4981 agtaagtact gtatccatgt ctgtcagctg catatggttc tgtacaaaat caatattgct
     5041 ggtatgttta actacccact taccgtcttc ttccactgca tgtgcatacc agtttaagaa
     5101 gttttcatca atagaaagtt ccagacctgc tgatgcgatc tttccttctt ccgggataaa
     5161 acggtcatag ccataccaat catagcggaa ggataagaaa aacaggacac ctgctgccac
     5221 acagcccatc agctgtattt tatgggaaaa cagcttctta aaatcagcct ggtaaatgat
     5281 ctcaataatg cagtgggcta aaaggatgcc aacaaccatt ccaaagagtc cccagcgcag
     5341 acggctctgt aaagtccaga aaaacatgga ggttcccata ccagctgcaa ggacaataag
     5401 gatacggatg ggtgccatgg tctttttaaa ggccattgcc tttcctgctg cttccatagg
     5461 acgttttctg taaagttcca gtcccaggat tgctcctgca caaatagcga tcaaaagtcc
     5521 caggatctga tctgcctcca ctgtctttcc agcggagata tctgccagag acataatgta
     5581 cgcgcctaca ggggataaat gacccagtgt attaaaaagc gcttcatttg gcacctggtt
     5641 ggccgtaatg aaaaatatgt cttcaaagcc ctcgatcaga gcaccaacta ccggaaagaa
     5701 actgaaaaat acaaccgtag ctaaaagtcc cactatgatc tttcccgtca tcatcattgc
     5761 cacaataacg gtctcatagg tcaccatgta atacagcagg ttcatgaaaa agccaaagag
     5821 cataggtcct gcaaattttg ccgggcttac gccgtatgca gcagctgccg ctgtaagcac
     5881 taaaagatgg ataagatagg tgactgccag tatgaggata ccatctacat gatataccag
     5941 atacaacatt tcccttctta caggaaggct gtggtaaaaa tctaccttct tctggttgtg
     6001 aagataaaca aaaccggctg caccgatcac taccgctgct gttaccatta aaaataatgg
     6061 cataacattt ccataggaaa ccagatccac taccgccttt gttttatatt ccagcacttt
     6121 tgccaggtat tccggtgttc cggtatctac cagaggcgta tatccgttgt acatggcaaa
     6181 ctggctgttt gctgcgtttt ccatgatcag cgccagattt acaggatagg caaagaaaaa
     6241 tcctaaaaac aacagggcaa tgacccagat cctgcgcttt aagtcttctt tttgcagttt
     6301 aaaaaataag cttgcggatg tcataacctg ccacctccgt ttcactgatg aatatttcct
     6361 ctaaggacag agggatacac tcaaaaaata ctggctcata ccggttcata atgtcctctg
     6421 cttcctgttt tgtgcctctt actgtaaggg taagaagccg tcctcttcct tcctgtgaaa
     6481 ggatctgaag tccctttaag tcatctgc
//
LOCUS       DBMDUA010000155         6468 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4941_length_6468_cov_1.693105, whole genome shotgun sequence.
ACCESSION   DBMDUA010000155 DBMDUA010000000
VERSION     DBMDUA010000155.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4941_length_6468_cov_1.693105"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="ACZPCL_10535"
     CDS             complement(<1..785)
                     /locus_tag="ACZPCL_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013740.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR1162938.1"
                     /translation="MKNSKFRFFMDWGLTALAVIASCVLLCFALLKFSVIAAIIKKII
                     TILMPIIYGGVLAYLMLPVYNRTEVCVRDLLSKVIKNDEGRTRIAKSIATLVSLAVLF
                     AIVSGLVSMLIPQIYESIIGFQDSLTEYINNLTVWISKLLQDNEHLQESIMPYYNEAV
                     MRFQNWIATDLVPNISLIFDRVSSGVISLVTVLKNLLIGLIVMVYFLNIKDTLCAQSK
                     KMVYGFLNVRAANRLIGEVRFAHKIFGGFITGKLLDSLIIGIM"
     gene            975..1298
                     /locus_tag="ACZPCL_10540"
     CDS             975..1298
                     /locus_tag="ACZPCL_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3837 domain-containing protein"
                     /protein_id="HIR1162939.1"
                     /translation="MSYIIKMALDIKARFNPPAHMSSPIEAYCAIGTVAKALGLPCPQ
                     RKDTLFEMRQELSDAEAGKSFPSERIEKINQILMSFIRDEETTDAMMAYVTYGYENEN
                     GNAST"
     gene            complement(1314..1535)
                     /locus_tag="ACZPCL_10545"
     CDS             complement(1314..1535)
                     /locus_tag="ACZPCL_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162940.1"
                     /translation="MLGILFAFLFLICVFKFVGILLMMCGKVMGAVFSMLGFIISLAV
                     GVLLFGLSAAFSMILLGIAAVWLLVRIIL"
     gene            complement(1659..2822)
                     /locus_tag="ACZPCL_10550"
     CDS             complement(1659..2822)
                     /locus_tag="ACZPCL_10550"
                     /inference="COORDINATES: protein motif:HMM:NF045169.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR1162941.1"
                     /translation="MKLMGYKRENGTFGIRNHLLILPTSVCSSETAQRIAALVPGAVA
                     IPHQHGCCQVGADYLQTVNTLVGFGKNPNVGAVLVVSLGCEGIQPEVVADQIRESKKP
                     VETVIIQECGGTLGCIAKGAEIAAKMARELSVQNKVAFDISELVLGLECGGSDPTSGI
                     AGNPSVGAASDKLIQLGGTSILSETTELIGAEHLVAARCVTKEMGDKLTAMVKRIEDK
                     SKLYGVDLRGTQPTPGNIAGGLTTIEEKSLGCIHKAGQSPVIGVLEYSEAVDPEKHGL
                     YFMDTPGEDIDSITGMVAGGAQIILFTTGRGTPTGSPVAPVIKVTGNPETYAKMTDNI
                     DINAGRVITEGKTPQDIGAEIFDKMVAVANGELTKAEVLGHKEFGIYRIGDTF"
     gene            complement(2842..3114)
                     /locus_tag="ACZPCL_10555"
     CDS             complement(2842..3114)
                     /locus_tag="ACZPCL_10555"
                     /inference="COORDINATES: protein motif:HMM:NF020250.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR1162942.1"
                     /translation="MKNYVYMTDKDNVVTMIRQLNAGEAIEVDGVKIKANQDIPVYHK
                     IAIEEIKTGETVVKYGESIGTASCDIHIGDWVHTQNLESGRGRGDR"
     gene            complement(3167..3682)
                     /locus_tag="ACZPCL_10560"
     CDS             complement(3167..3682)
                     /locus_tag="ACZPCL_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162943.1"
                     /translation="MMVGENIHNAFVVVFKTLQEIEKFIGKCRTELDTKKYYMPAERF
                     MRYSSDQTWEGWIYWSFILLFQRLDDGSVMENGWIDGPVYAVEINVDSDTCDVPKVYI
                     AKMQFDRMKDWTKGCSPSNHSLFYNAIHGNSWTPFWGLQKVEKQEHDLTDLTQKNYKD
                     VIFGTIEKMAK"
     gene            complement(3696..4349)
                     /locus_tag="ACZPCL_10565"
     CDS             complement(3696..4349)
                     /locus_tag="ACZPCL_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162944.1"
                     /translation="MIAEIYHKFSTDLEDVLTGDFFGAMRYMPFNRGLNQIFKNYAVS
                     EDPQVTHILSNAADDDFNFEFWKRSENGLVEIDGFIPLTSVEIGIEVKYRSGLSGGDQ
                     LEKEAQVLDEWCNGKEKLLILIGEAEEAKAIYIENKDKRVFRDVHLAYLSWQDILLGL
                     DQVLISSSYEKKMIEDLKTLLREKGFVSFEGFSIDQPVVDKDICWTMDGNDFVKSDA"
     gene            complement(4396..5784)
                     /locus_tag="ACZPCL_10570"
     CDS             complement(4396..5784)
                     /locus_tag="ACZPCL_10570"
                     /inference="COORDINATES: protein motif:HMM:NF027442.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HIR1162945.1"
                     /translation="MVREVITVAALTAAFSFLGGCTPRAATLPGFRSETKEFSQSGET
                     FGNPLMGYAPDARRENVSEDITLLYVDITWKELEPECGVYDWDTIEKENQFFRWRSEG
                     KHLVLRFICDDPGDESHTDIPDWLYRETGKDGDWYDNDYGKGFAPDYSNTTMIRRHTQ
                     AVKAMGERWGSDSLISYIELGSLGHWGEWHVYYGAGIRRLPLEEVREQYVKVWPEAFP
                     NAMILMRRPFCSAKKYGFGLYNDMAGHPEDTKTWIEWIENGGVYDQTGETDVIVPMNS
                     FWKKAPVGGELNSSISMRRMLVKNLNETAELIRSSHTTFLGPKIADKSYLDGYQTLLK
                     NMGYRIWVTQAQLKQALGKATLILTWENDGTAPFYKNWPVVVTVTDSSGAVIEEKTLD
                     WKISELMPGEKNVTTVKLDTRGLLDKARSDRNICVEIRDPMTEKAAVRLANKELDQKA
                     QKKQMIVFKSGE"
     gene            complement(5777..>6468)
                     /locus_tag="ACZPCL_10575"
     CDS             complement(5777..>6468)
                     /locus_tag="ACZPCL_10575"
                     /inference="COORDINATES: protein motif:HMM:NF027640.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HIR1162946.1"
                     /translation="NKWEEIMSVKDTIKKSVLESGTFAQYDMLKIMLAMTIALLMGCL
                     IYMVYKKFYSGVVFSRSFAVTLVGMCVLTCMVTLAISTNIVISLGMVGALSIVRYRTA
                     VKDPMDLLYLFWSITTGITAGAEMYPLVWGAAIIMIVMLTLFYHYQEKGKVYIAVNHY
                     QGDVAGDEAIRCFGKRKYFLKSKTMRKGMTEMAIEVFCKKEDTSFMEKIRAVEGVNDV
                     TLIQYNGEYHG"
BASE COUNT         1786 a         1637 c         1067 g         1978 t
ORIGIN      
        1 cacatgatgc ctatgatcag agagtctaaa agctttcctg taataaagcc gccaaatatc
       61 ttatgggcaa aacggacttc accgatcagt cggttagctg ctcttacatt taaaaatcca
      121 taaaccattt tttttgactg ggcacacagt gtatctttta tgtttaaaaa gtacaccata
      181 acaataaggc cgatcagcag attttttaaa acagtgacca gactgatcac accgctggaa
      241 acgcggtcaa agatcaggga aatatttggc accagatccg tagcgatcca gttctggaaa
      301 cgcatgaccg cctcattgta atacggcata atgctttcct gaagatgttc attatcctga
      361 agaagtttgc tgatccagac ggtcaggttg ttgatatact ctgtcaggct gtcctggaaa
      421 ccaatgatac tttcgtagat ctgtgggatc aacatggaaa ccagtccgct gacgatcgca
      481 aacaatactg cgagactcac caacgttgct atactttttg caatacgggt gcggccctca
      541 tcatttttga tcacctttga cagaagatcc cggacacaga cttctgtcct gttgtaaacc
      601 ggcagcatca gatatgccag tacacctccg tatataatag gcataaggat cgttatgatc
      661 ttttttatga tcgctgcgat cacggaaaat ttcagtaatg caaaacataa aagaacacaa
      721 ctggcgatca ccgctaatgc cgtaagaccc cagtccataa aaaatcgaaa tttggaattt
      781 ttcatatttt agttcaggct cctatgttac tttcgtcatt tgtatgagta taccatacta
      841 tacccgattt taaaagcaga ccggacgtta aaaaaatgtt tttaatttca ggatcgttct
      901 gttaaaacag atggttctgt gttaaaatac tatgcacatg catttatatc attttacaga
      961 aagaaggcga tcttttgtct tatatcataa aaatggctct agatatcaaa gcccgtttta
     1021 acccaccagc ccatatgtcc tctcccattg aagcatactg cgccattgga acagttgcaa
     1081 aagcccttgg tcttccctgt ccacagcgta aagatacgtt atttgaaatg cgccaggaac
     1141 tttctgacgc agaagcagga aaaagttttc catccgaacg gatcgaaaag atcaatcaga
     1201 tccttatgag ttttatccgt gatgaagaaa caaccgatgc catgatggct tatgttacat
     1261 atggttatga aaatgaaaac gggaatgcct caacataagc actcccgttt ttattataag
     1321 atgatccgca ctaacagcca cacagctgca attcccaaaa ggatcatgga aaaagctgcc
     1381 gaaagcccaa acagcaatac tcctactgcc agtgaaatga taaatcccag catgctgaat
     1441 acagcgccca ttacctttcc gcacatcatc agcaggatac caacaaattt aaacacacag
     1501 atcagaaata aaaaagcaaa taaaattcct aacattttat ctaatatcct ttgacctttc
     1561 taataaaaca tctactacaa accgatcgat cacccatgaa gctgctacag acaacaaggc
     1621 acaccagaag atcggtaaaa ccacctgcag atactaactt agaacgtatc tcctatgcga
     1681 tatattccaa attccttatg tccaaggact tctgccttgg tcagttcgcc gtttgccaca
     1741 gcaaccatct tatcaaaaat ttcagcacct atatcctgtg gtgtctttcc ttctgtgatg
     1801 accctgccgg catttatatc gatattatct gtcatctttg cataggtttc aggatttccc
     1861 gtgactttga taacgggcgc aaccggactg cctgtaggag ttcctctacc tgtagtaaaa
     1921 agaatgatct gtgctccacc tgcaaccata ccggtgatgg agtcaatgtc ttcccccggt
     1981 gtatccatga aataaagacc atgtttttcc ggatcaaccg cttctgaata ctccagaaca
     2041 ccgatcacag gactctgacc tgctttatga atacagccca gggatttttc ttcaatcgtt
     2101 gtaagtccgc ctgcaatatt acctggcgta ggctgtgtcc cccgaaggtc tactccgtaa
     2161 agttttgatt tatcttcaat acgcttgacc atagctgtaa gcttatcacc catttctttg
     2221 gtcacgcaac gggcagctac cagatgctct gcacctataa gttctgtggt ctcgctgagt
     2281 atacttgtac ctccaagctg gatcagctta tcggaagccg cacctacaga cggattacct
     2341 gcaataccac tggtaggatc actgcctcca cactccagtc ctaaaacaag ttcgctgata
     2401 tcaaacgcaa ccttattctg aacggaaagt tccctggcca ttttagctgc aatttcagca
     2461 cctttggcaa tgcagcctaa ggtaccgcca cattcctgga tgataacagt ttcaaccggc
     2521 tttttacttt cgcggatctg atctgctact acttccggct gtattccttc acagccaaga
     2581 ctgacaacaa gtacagcacc tacattcgga tttttgccaa agccaaccag ggtatttaca
     2641 gtctgaagat aatctgcgcc tacctgacag cagccatgct gatgaggaat tgcaactgct
     2701 cccggcacta aagcagcaat tctttgtgca gtctcgctgg aacagacgga agttggcagg
     2761 atcaacagat gatttcttat tccaaaagta ccattttctc ttttgtatcc cattaatttc
     2821 ataattatgt ttccctttcc tctaacggtc accgcggcct ctgcctgact ctaaattctg
     2881 ggtatggacc cagtcaccta tgtggatatc acagctggca gtaccgatgc tctcaccata
     2941 ttttacaacc gtctcccctg tttttatttc ttcgatcgca attttgtggt atacaggaat
     3001 atcctggttt gctttgattt ttacgccatc tacctcgatt gcttctccgg catttagctg
     3061 gcggatcatg gttactacat tatccttgtc ggtcatgtat acataatttt tcattcattt
     3121 tctcctgtta aatttatttt ttaagtttat tataaatgga tgtattctat ttcgccatct
     3181 tttcaattgt cccaaaaata acatccttat aatttttctg tgtaagatcc gtcagatcat
     3241 gttcctgctt ttccactttt tggagtcccc agaaaggtgt ccaggaattt ccatggattg
     3301 cgttataaaa caatgaatga ttggatggag aacatccctt tgtccagtct ttcattctat
     3361 caaattgcat ttttgcaata taaacctttg gaacatcgca tgtatcagaa tctacattga
     3421 tttctaccgc atataccgga ccgtcaatcc agccattttc cataacagag ccatcatcca
     3481 ggcgctgaaa cagcaatata aaactccagt agatccagcc ttcccatgtc tgatctgagc
     3541 tgtatctcat gaaacgttct gccggcatgt aatatttttt cgtatccagt tctgttctgc
     3601 atttaccaat aaacttttca atttcctgca atgttttaaa tacaaccaca aaagcattat
     3661 gtatattttc tccaaccatc atttattctt tccccttaag catcgctttt tacaaaatca
     3721 tttccatcca ttgtccagca gatatcttta tctaccactg gctgatcaat tgaaaaacct
     3781 tcaaaggata caaacccctt ctctcttagc aacgttttta aatcttctat cattttcttt
     3841 tcatatgatg atgatatcaa tacctgatcc agtcccagta aaatatcctg ccacgataaa
     3901 tatgccagat gaacatcccg gaatacccgt ttatccttgt tttcaatata aattgctttt
     3961 gcctcctctg cctccccgat caatattaag agtttttctt taccattgca ccattcatca
     4021 agaacctgcg cttctttctc caattgatcc ccaccagata atccactcct gtattttacc
     4081 tcgatcccga tttccacaga cgtaagcgga ataaatccat cgatctcaac cagtccattt
     4141 tccgaccgct tccagaattc aaaattaaaa tcatcatctg cggcattaga aagaatatgc
     4201 gtcacctgcg gatcttcact gactgcgtaa ttcttaaata tctgattcag gcctctatta
     4261 aaaggcatat acctcatagc accgaaaaaa tcaccagtca gcacatcttc cagatctgta
     4321 gaaaacttat gataaatttc agctatcata aaacattcct ttccttgttt ctttcttgtt
     4381 ctttctcacc attccttatt cacctgattt aaaaacaatc atctgttttt tctgtgcttt
     4441 ctggtcaagt tctttatttg caagccgtac agccgctttt tccgtcatgg gatcgcgaat
     4501 ttccacacag atattcctgt ctgaccgtgc tttatcaagc aggcctctgg tatcaagctt
     4561 cactgtcgtt acgttttttt ctcctggcat cagttcagat atcttccaat ctaatgtttt
     4621 ttcctcaatg acagccccgg aactgtctgt cactgtgaca actaccggcc agtttttata
     4681 aaacggtgcc gttccatcat tttcccatgt cagtatgagc gttgcctttc ccaatgcctg
     4741 ctttaactgt gcctgggtga cccagatccg gtatcccata tttttcaaca acgtctgata
     4801 tccatccaga taggatttat cagcgatctt cggacccaga aatgtggtat gagatgagcg
     4861 gatcagctct gccgtctcat tcaggttttt aactaacatc cgtctcatac ttatggaact
     4921 gtttaattct cctcccacag gagccttttt ccaaaaactg ttcatgggta caataacatc
     4981 ggtttccccg gtctgatcat acacaccgcc attttcaatc cactctatcc atgtcttcgt
     5041 atcttcggga tggcccgcca tatcattgta cagaccaaat ccatattttt ttgcagaaca
     5101 gaatggtctg cgcatcagga tcatcgcatt cggaaatgcc tccggccata ccttcacata
     5161 ctgctccctt acttcttcca acggcaatct gcgaattcct gccccataat aaacatgcca
     5221 ttccccccag tgaccaaggc tgcccaattc tatataactg atcaacgaat ctgaacccca
     5281 gcgttctccc atagctttta ccgcctgtgt atggcgtctg atcatggtcg tattagaata
     5341 atccggcgca aatccttttc cataatcatt gtcgtaccag tctccgtctt tccctgtttc
     5401 ccggtacagc cagtctggaa tatctgtatg actctcatct ccaggatcat cacagataaa
     5461 tcttaatacc agatgttttc cctctgatct ccaacgaaaa aactgatttt ctttttcaat
     5521 ggtgtcccag tcatataccc cacattccgg ttccagctct ttccatgtta tatctacata
     5581 caaaagtgtg atgtcctctg atacattttc ccttctggca tccggtgcat atcccattaa
     5641 tggatttcca aatgtttccc cgctttgtga aaactctttt gtctcacttc taaaaccagg
     5701 cagtgtcgcc gcccgtgggg tgcagccacc caaaaaagag aaggctgctg taagagctgc
     5761 tactgtaatt acttccctaa ccatgatatt ctccattata ctgaatcaaa gttacatcat
     5821 ttacaccctc tactgcacgg atcttttcca taaaactcgt gtcctctttc ttgcagaata
     5881 cttcaattgc catctcagtc atcccctttc gcatggtctt tgatttcaga aaatatttcc
     5941 gttttccaaa acagcggatt gcttcatcac ctgccacatc accctgataa tggttcactg
     6001 caatatatac ttttcctttt tcctgataat gataaaacaa tgtgagcatc actatcataa
     6061 tgatcgctgc tccccaaacc aagggataca tttctgctcc tgctgtgata cctgtggtaa
     6121 tactccagaa aagatacaaa agatccatag ggtcttttac ggctgtacga taacgcacaa
     6181 tcgataatgc tccgaccata ccaagggaga ttacaatatt ggtgctgatc gccagagtta
     6241 ccatacatgt cagcacacac attcccacta aggtcaccgc aaaacttctt gaaaacacaa
     6301 ctccggaata aaacttttta tataccatat agatcaggca tcccattagc agggcaattg
     6361 tcatggcaag catgatcttt aacatatcat actgtgcaaa tgtgcctgat tccaggacag
     6421 attttttgat cgtatctttt acactcataa tttcctccca tttattct
//
LOCUS       DBMDUA010000156         6396 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_4989_length_6396_cov_3.290548, whole genome shotgun sequence.
ACCESSION   DBMDUA010000156 DBMDUA010000000
VERSION     DBMDUA010000156.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6396)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4989_length_6396_cov_3.290548"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="ACZPCL_10580"
     CDS             <1..172
                     /locus_tag="ACZPCL_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HIR1162947.1"
                     /translation="AIQEYANITELDAATLNRLIKEIVVHERIDEDKIRHISIEIHFN
                     LKPIPEVEQVTA"
     gene            complement(196..975)
                     /locus_tag="ACZPCL_10585"
     CDS             complement(196..975)
                     /locus_tag="ACZPCL_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024136.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HIR1162948.1"
                     /translation="MKKIFAAGVIMSILSVLLSLCARKVTGFAQAYSVTIYPVLKGIF
                     ARIFGIFPFSVSEVGLYLLLLLCIFSVVKWFKTPLYLLNGAFFTASLLFFLFTINCGI
                     NYYRNSFSYEAGFVIRTHSTDELYALCSNLVNKVNENIPTETDPSPSDAGNFSYFQNF
                     SYLREMGKEGQNAMKGLANEYPQFKSWYPYPKPLIHPRLLSVQQLTGVYSPFTVEANY
                     NSEIPAYNIPHTICHELFYPQLGVKLCSMAGGGVQKSIWCF"
     gene            1112..1507
                     /locus_tag="ACZPCL_10590"
     CDS             1112..1507
                     /locus_tag="ACZPCL_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HIR1162949.1"
                     /translation="MGYQDDYVMRTISDLVRAIARLALGKNEINYALPDTEDKYSDTD
                     RIYRKLRDLVDAGEINEAENQLYENLDENDTEHLEMALTFYMYLNQLDDDTLFMANYS
                     REEIVEGINSVSASFGITGFENFVDTTMV"
     gene            1679..3646
                     /locus_tag="ACZPCL_10595"
     CDS             1679..3646
                     /locus_tag="ACZPCL_10595"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUV3 family DEAD/DEAH box RNA helicase"
                     /protein_id="HIR1162950.1"
                     /translation="MEIKEECLAYLGQYYKELFFGTANKRYRSMTGADLKHRLNRLSQ
                     ETLNGVEKPNELNDIHAMFAKVCAYLMHKEERLKPGPALDQLKENWKKMEDFCGMLAA
                     KDMEYLPELTEEELEHVLKMQGIRRYLLTNSLERAYQLFYLPKTIKKGIRESVKRRPE
                     DEYPETRAMKRRFILHIGPTNSGKTHDALERLKTASHGAYFGPLRLLALEVYDRLNGE
                     NIPCSMITGEETLEVAGACCQACTVEMLNEHEYFDVAVVDECQMVADPYRGHNWTRAV
                     LGLRAEEIHLCMAPEAEDIIVQMIKRCGDTFKIVRHKRNTRLTLEEKPYSLKKDLKKG
                     DALIVFSKKSVLALAAHLENQGVHCSVIYGSLPPATRREQVRRFLEKETEVVVSTDAI
                     GMGLNLPIRRIVFIETRKFDGVGRRSLLPEEIKQIAGRAGRFGLYDEGFVAVLDDLEL
                     IKDGLERRPIPIMKAYIGFPEQLLKLPAEIDTLVKIWASMETPSIYEKMEVDELLALY
                     VSFEHVQRDHMDEFSKEEIYKLITCSVDIDNKLVMDLWKDYCRDYRDVEELQFPYSPG
                     PDLYDLESYYKMLDLYFQFSRKVGLPVQEENLVEERRNTEEEISRILKTECASYTRKC
                     TICGRELSWDYPFSICERCFERGKRVHPRRR"
     gene            3704..4960
                     /gene="uraA"
                     /locus_tag="ACZPCL_10600"
     CDS             3704..4960
                     /gene="uraA"
                     /locus_tag="ACZPCL_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HIR1162951.1"
                     /translation="MEKRIIQVEEKVPVKLLIPLSIQHMFAMFGASVLVPFLFGISPA
                     IVLFMNGIGTLLFIAITKGKAPAYLGSSFAFLAPASIVIQNFGYEYALGGFVAVGFCG
                     CILALIIYKFGTDWINIVLPPAAMGPVVALIGLELSANAASNAGLLDENIDPRKIIVF
                     MVTLGVAVFGSILFRKFLAVIPILIAVLAGYAAAIICGIVDFEEVAAAKIIAMPHFSP
                     PKFDAQAIMIILPVILVIISEHIGHQVVTSKIVGRDLLKDPGLHRSLFADNFSTMLSG
                     MIGSVPTTTYGENIGVMAVTGVYSVRVIAGAAILSIICSFVGKLSALISTIPGPVIGG
                     ISFLLYGMIGTSGLRILVDSKVDYSKNRNQMLTSVIFVTGLSGAALRIGNIELTGMVL
                     ACVVGMAMSLVFYVLDRLKLTNDQAE"
     gene            complement(5048..5281)
                     /locus_tag="ACZPCL_10605"
     CDS             complement(5048..5281)
                     /locus_tag="ACZPCL_10605"
                     /inference="COORDINATES: protein motif:HMM:NF015956.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HIR1162952.1"
                     /translation="MVEPLSGLVEGEMAEVVWIISEADVREMLGRSGFLYGEVLTCLL
                     KNPARQLCIYRIRRRVVAIREEYTKEILVRRML"
     gene            complement(5349..6113)
                     /gene="sfsA"
                     /locus_tag="ACZPCL_10610"
     CDS             complement(5349..6113)
                     /gene="sfsA"
                     /locus_tag="ACZPCL_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HIR1162953.1"
                     /translation="MTYDNIVTGTFLKRPNRFIAYVEVKGDVKTCHVKNTGRCQELLV
                     PGCTVVLEFHPKAKEMKRKTEYDLIAVYKGNLLINMDSQAPNQAALEWIREKEAKGLS
                     LENVGIPTNIRREVTHGESRFDLAFELKDEVTGKTVPAFMEVKGVTLEKEGNVFFPDA
                     PTIRGVKHLNGLIQAKKDGFETFLLLVIQMKGIRKFYPNDETHKAFGDALRAAAAAGV
                     HILAYDCIVTENSMDMDEKVEVGLLYDNDTRTHCEL"
BASE COUNT         1842 a         1425 c         1445 g         1684 t
ORIGIN      
        1 agccattcag gaatacgcca acatcacaga gctggacgca gccaccctta accgcttaat
       61 caaagaaatc gtcgtgcatg agcgcattga cgaagataaa ataagacaca tttctatcga
      121 aattcatttt aatctcaaac ccatcccgga ggtggaacag gtcactgcct gacctgtcct
      181 gccgggacgg ttctcttaaa aacaccatat agatttttgt acgccgccgc ccgccatcga
      241 gcagagtttt acacctaatt ggggataaaa cagttcatga cagatggtat ggggaatgtt
      301 gtaggcaggt atctcactgt tgtagttggc ttctactgta aaaggagaat agacgccggt
      361 cagctgttgg acagataaaa gccttggatg gatcagaggc tttggatatg ggtaccagct
      421 tttaaactgg ggatactcat ttgccagtcc tttcatggcg ttctgccctt ctttccccat
      481 ttcccgaaga taggaaaaat tctgaaaata agagaaattc ccggcatctg aaggagatgg
      541 gtctgtttct gttggtatat tttcatttac cttatttaca agattactgc aaagagcata
      601 gagttcatcg gtagaatggg tccggatcac aaaaccagct tcataagaaa aagaattccg
      661 gtaataatta atgccgcagt tgatggtaaa taagaaaaac aggagagaag ctgtaaagaa
      721 tgcaccattt aacagatata atggcgtttt aaaccatttg accacagaaa aaatacaaag
      781 gagcagaagc agatacagcc ctacttctga tacagaaaag ggaaagatcc cgaaaatacg
      841 cgcaaatatc cctttcagga caggatatat agttacagaa taagcctggg caaagccggt
      901 taccttcctg gcgcaaaggc ttaacaaaac agaaaggatg ctcatgatca caccggcagc
      961 aaatattttt ttcaagttca cgacctcctt cagatcattg cggatacccc cattataaat
     1021 gcttttgcct tgactgaaaa ggtggtttca tctatactga aatgagctaa gaagagaaaa
     1081 agttatataa atataaaaag gggaaacaga tatgggatat caggacgatt atgtaatgcg
     1141 taccatcagt gacttagtcc gcgccattgc aagactggca cttggaaaaa acgagatcaa
     1201 ttatgccctt ccggatacag aggataaata cagcgataca gaccgtattt atcggaaact
     1261 gcgtgatctg gtagatgcag gtgagatcaa cgaagcagag aaccagcttt acgaaaatct
     1321 ggatgaaaac gacacagaac atctggaaat ggcactgact ttctatatgt atttaaacca
     1381 gttagatgat gatacacttt tcatggcaaa ctattccaga gaagagatcg tagaagggat
     1441 caacagtgtc agcgcaagct ttggcattac cggatttgaa aattttgtag ataccaccat
     1501 ggtttagcca gatgacggtg cggatcataa agttccgcgc acttacaggc gggaccacaa
     1561 gaaacacagg gggctgctgc aggatgcctt aaaattttgc agcagcccct tgtggcggat
     1621 ataagaaaac ggacatgtca cctgagggtg atatcgccat gaataaaaga ggacatccat
     1681 ggaaataaaa gaagaatgtc tggcctatct ggggcagtat tataaagagc tgttttttgg
     1741 aacagccaat aaacgatacc gctccatgac aggcgcggac ttaaaacacc gcttaaatcg
     1801 tttaagccag gagactttaa acggggtgga aaagccaaat gaattaaacg atatccatgc
     1861 catgtttgca aaagtgtgtg cctatctcat gcacaaggaa gaacgtttaa aaccgggccc
     1921 ggccctggac cagttaaaag aaaactggaa aaaaatggaa gatttctgcg ggatgctggc
     1981 agcaaaagat atggaatacc tgccggagct gacagaagaa gagctggagc atgtattaaa
     2041 aatgcagggc atccgccgct accttcttac caactcctta gagcgggctt atcagctgtt
     2101 ttatcttcca aagactatta aaaagggaat acgggagtct gtaaaacgcc gcccggagga
     2161 tgaatatccg gagaccaggg ccatgaagcg ccgctttatc cttcatatcg gaccaacaaa
     2221 cagcggaaag acccacgatg cattagaaag gttaaaaaca gcttcccacg gcgcctattt
     2281 tggtccctta agacttctgg cactggaagt ttatgaccgg ctaaacggtg aaaacattcc
     2341 ctgttccatg ataaccgggg aagaaacact ggaagtggcc ggtgcctgct gtcaggcatg
     2401 tactgtggaa atgttaaatg aacacgaata tttcgacgta gctgtagtgg acgagtgcca
     2461 gatggtagcg gatccctaca gaggccacaa ctggaccagg gcagtcttgg gacttcgtgc
     2521 agaggagatc catctgtgta tggctccgga ggcggaagat attatcgtgc agatgatcaa
     2581 acggtgtggg gataccttta agatcgtccg tcataagcga aatacaaggc ttaccttaga
     2641 agaaaagccg tattccctga aaaaagattt gaaaaaaggc gatgctctta tcgtattttc
     2701 aaagaaatca gtactggcgc tggcggccca cctggaaaac cagggagtcc attgtagcgt
     2761 tatctatgga agtctgcctc ctgccaccag aagagagcag gtgcgccgct tcctggaaaa
     2821 agagacagaa gtggtagtca gtaccgatgc cattggaatg ggcttaaacc tgcccatccg
     2881 ccgtattgtt ttcattgaga cccggaaatt tgacggagta ggccgcagaa gccttctgcc
     2941 agaggagatc aagcagatcg caggtcgggc cgggcgtttt ggtctctatg acgaaggttt
     3001 tgtggccgtg ctggacgatc tggaattaat aaaagacgga ctggaacggc gtccgatccc
     3061 cattatgaaa gcctacatcg gcttcccgga acagctgtta aagctgccgg ctgagatcga
     3121 cacactggta aaaatatggg ccagtatgga aacaccttct atttatgaaa aaatggaagt
     3181 agacgaactg cttgcgctgt atgtaagctt tgagcatgta cagcgggatc atatggacga
     3241 attttccaaa gaagagatct ataagctgat cacctgctct gtggacattg acaataagct
     3301 ggtcatggat ctgtggaaag attactgccg ggactaccgg gatgtggaag aactgcagtt
     3361 tccatacagt ccgggaccgg atctgtatga tctggaaagc tattataaaa tgctggatct
     3421 gtatttccag ttttccagaa aagtgggact gcctgttcag gaagaaaatc tggtggaaga
     3481 gcgcaggaac acagaagaag agatcagccg tattttaaag acagaatgtg ccagctacac
     3541 ccgaaaatgc acaatctgtg gaagagaact ttcctgggat tatccgtttt ccatctgcga
     3601 acgctgcttt gaacgtggaa aaagagtaca tcccagaaga agataatatt aatgtactcc
     3661 tgggattttt ccgggggcaa ataacacatc aaaggagaag agaatggaaa agagaattat
     3721 tcaggtagaa gaaaaagttc ctgtaaaatt gctgataccc ttaagtatcc agcacatgtt
     3781 tgccatgttc ggcgcgtccg ttctggtgcc tttccttttt ggcatcagcc ctgcgatcgt
     3841 actgttcatg aatggtatcg gaaccctttt atttatcgcg atcacaaagg gaaaagcacc
     3901 ggcttactta ggttccagtt ttgctttcct ggcacctgca tctattgtga tccagaattt
     3961 tggctatgaa tacgctttag gcggtttcgt ggcagttggt ttctgcggct gtattttagc
     4021 acttattatc tataagtttg gtacagactg gatcaacatc gtacttccgc ctgcagccat
     4081 gggcccggta gtagctctga tcggtctgga attatccgcc aatgcagctt ccaacgcagg
     4141 tctgctggat gagaacattg atccaagaaa gatcattgta tttatggtaa ctttaggcgt
     4201 agctgtattc ggttccatcc tgttccgcaa attcctggca gttatcccaa tcttgatcgc
     4261 agttctggca ggctatgcag cagccatcat ctgcggaatt gttgattttg aggaagtagc
     4321 cgcagctaag atcatcgcta tgcctcattt ttccccaccg aaatttgatg ctcaggcgat
     4381 catgatcatc cttcctgtta tcctggtcat catttccgag catatcggac atcaggtagt
     4441 taccagtaag atcgtaggaa gagatctgtt aaaagatccg ggacttcaca gatccttatt
     4501 tgcagataac ttctctacca tgctttccgg tatgatcggc tccgtaccga ctactactta
     4561 tggcgagaat atcggtgtaa tggcagtaac cggtgtatac agtgtccgtg tgattgcagg
     4621 cgcagccatt ctttctatca tatgttcctt cgtaggaaag ctgtccgccc tcatcagcac
     4681 catcccgggt cctgtgatcg gcggtatctc cttcctgctc tacggcatga tcggaacctc
     4741 cggcctgcgc atcctggtag actccaaagt agactactcc aaaaaccgca accagatgct
     4801 caccagcgtt atcttcgtaa ctggtctttc cggcgcagca ctgcgtattg gtaatatcga
     4861 acttaccgga atggtacttg cctgcgtagt aggtatggca atgagccttg tattctatgt
     4921 attagacaga ttgaaactga ccaatgatca ggcggagtaa aatgtttttg gccttgcggc
     4981 attgctatct gacaacatta cgaaccggat aaaaaatagg cgattgtggt ttcacagtcg
     5041 tctattttca tagcatccgc cgtacaagta tttcttttgt atattcctcc cgtatagcca
     5101 ccacccgcct gcgtattctg tatatacaca gctgccgggc cggatttttt aacagacagg
     5161 tcaggacctc tccgtacaga aatccgcttc ggccaagcat ttctcttaca tctgcctctg
     5221 agatgatcca tactacctca gccatttcac cttcgaccag gccggataaa ggttctacca
     5281 cagtttcacg tccaatattc ccactctttt aaatatatgc gtgttatggt tggctatatg
     5341 atgctggatt acaattcaca atgtgttctt gtatcattat catacagtaa tccaacttcc
     5401 actttttcat ccatatccat agaattttcc gtcacaatac aatcatatgc taaaatatgt
     5461 accccggctg cagctgctgc ccgcagggca tccccgaaag ccttatgtgt ctcatcatta
     5521 ggataaaatt tccggatccc tttcatctgg atcacaagga gaagaaaggt ctcaaagcca
     5581 tccttttttg cctgtatcag cccgtttaaa tgtttcacgc cccggatcgt aggtgcatcc
     5641 ggaaagaata catttccctc tttttccaga gttactccct ttacttccat aaaagcagga
     5701 actgttttac cagttacctc atcctttaat tcaaaagcca ggtcaaaccg ggactcacca
     5761 tgagtgactt cccttcgtat attcgttgga attccaacat tttctaaaga aagccctttt
     5821 gcctccttct cacgtatcca ctctaaagca gcctgattag gcgcctggga gtccatattg
     5881 atcagcagat tccctttata aacagcaatc aggtcatatt ccgttttccg cttcatttcc
     5941 tttgccttgg gatgaaattc cagaaccacg gtacatccag gtactaaaag ctcctggcag
     6001 cggccggtat tttttacatg gcaggtctta acatccccct ttacctccac ataggcgata
     6061 aagcggttag gacgttttaa aaaggttccg gttactatat tatcgtatgt catatctata
     6121 cctcgcaagc atttgcattt atggtgctgt catcattcat ttgccatgga gtataacaca
     6181 aaagaaaaca caaaaaaagc ttttgcaaaa aaggcattcc tcattttaca aaagctttta
     6241 taaattgtat cattattgca gctttggtgt gccatatctt tcagaccaga tctttaaact
     6301 tcctttttac cagttctgcg atcatatcgg cagttccttc gatccccaat aaagaagaat
     6361 cgatcataag gtgtttatgc aggggatcat aaggtg
//
LOCUS       DBMDUA010000157         6357 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5025_length_6357_cov_1.932864, whole genome shotgun sequence.
ACCESSION   DBMDUA010000157 DBMDUA010000000
VERSION     DBMDUA010000157.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5025_length_6357_cov_1.932864"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2231
                     /locus_tag="ACZPCL_10615"
     CDS             <1..2231
                     /locus_tag="ACZPCL_10615"
                     /inference="COORDINATES: protein motif:HMM:NF039178.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HIR1162954.1"
                     /translation="GVDTDTFMKAFQTISDYLPESVTSFKVTFGAPQKAGGYTVIAAV
                     VDPNYEAAVGIGSLIILPKTENIELRWTQDFSVLSYKDIPLNDDGSLDEEQAMKLFGA
                     VLMDKETETVLDSSKVRYLFVGKDGDKEFYRSNKPSAKPGVYTETAYVLNGNNMDWPI
                     VRTYVIGRTAALIQFKDGQMETAPKEDKEYGTSEALFATKHVTYNGEAQEFKAGLYLA
                     ATPETEIEDADIQYRYYDVVTGTYLEGLPVNAGIYRVTADFKGNGQYTDAADQTGYLI
                     IEKAQPKFVLEEQQNFTYNGEAQKIMVQSVEDQDPETKYSVLYKNDQTDENASLEAPV
                     HAGTYRAILDLPETANYAAAHAEVIMNIIPAKATIEIHPVSFSFDEQTHGGSYLVYGI
                     RDEIPEHEVSYSTDNEIFVADEPMAVGTYAMRIQITDPDYENDVETQEDAVVIKEKEI
                     VPVIAEACLDIQYVCNGQIVGHQLVKVEGNSGDLCLFTDENVVLEIPEGYEKEAGFGS
                     IEVPYGETAQTIVSVKLKETQDDNEKPGDNGDNDNSGDNDDNGSNDNNGNSGNGGNGG
                     SSSSSDGSSSSSRKTIISESGSWRQDEKGIWFERAASGYPKNCWSFIDGKWFHFNAEG
                     YLTLGWYLSEDGKWYYCAPDGTLGTPIGAMYTGWLKDGADGYWYYLDPNTGEMLTGWR
                     EIKGQWYYLNPETLGDSGWMQDSSTKQWNYNVKAVMPKGALYVNTQTPDGYFVDENGV
                     RR"
     gene            complement(2326..3396)
                     /locus_tag="ACZPCL_10620"
     CDS             complement(2326..3396)
                     /locus_tag="ACZPCL_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1162955.1"
                     /translation="MATTLPYYEQKDIENIKKLREMTKTLPPFCTEFFRGIEPRTSTR
                     TRIAYAYDLSVFFDFLKKENPVFSKMERMDFTLDHLDQITVTDLEEYMEYLKYRFNEH
                     NQEIVNKERGIMRKISSLKSFYNYFFRTEKLKTNPAALVQLPKLHEKEIIRLDIDEVA
                     LLLDAVEQGDGLTDKQKAFHNRTKLRDLALLTLLLGTGIRVSECVGLDINDIDFKNGG
                     IHIHRKGGKEVTVYFGTEVETALQDYLDERNDIIPEEGSENALFLSLQKKRMNVRSVE
                     NLVKKYAKIVTPLKKITPHKLRSTYGTNLYRETGDIYLVADVLGHSDVNTTKKHYAAL
                     EDERRRSARNAVKLRENQGITP"
     gene            complement(3458..4183)
                     /locus_tag="ACZPCL_10625"
     CDS             complement(3458..4183)
                     /locus_tag="ACZPCL_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HIR1162956.1"
                     /translation="MKLQRLLSLLRQAIDQYQMIENGDHIAIGISGGKDSLTLLYGLS
                     QLQKFYPKHFTLSAITVDMGLDTMNLEPIKALCADFNVPYEIISTEIGKILFEARKES
                     NPCALCAKMRKGALNQKALELGCNKIAYAHHKDDLIETALMSLLYEGRFYAFPPVTHL
                     DRTDLTVIRPLMLVSEADVKGFRNKYQLPVCKNPCPMDGHTHREYVKNLIRTLNMDSP
                     GVRERLFHAVINGNFEDWPRLSK"
     gene            4542..4838
                     /locus_tag="ACZPCL_10630"
     CDS             4542..4838
                     /locus_tag="ACZPCL_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162957.1"
                     /translation="MKFKESLKKYGCLPVATGLASVGLAVPAFAEGAAPTVVTASDWS
                     SVISALTAQISVSTIIAALATFVAAGIGIVFMWWGLRKGIRSLMAAFRKGRMSI"
     gene            4906..5592
                     /locus_tag="ACZPCL_10635"
     CDS             4906..5592
                     /locus_tag="ACZPCL_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR1162958.1"
                     /translation="MELNLLDGSLNPVNIGRVIKHNHDFYKSHPGYFRPEGLLVFCGE
                     QGSGKTLSAVQYVKKLCSEYPDAILCTNVQISGLNPSTHVVEYDGLDCLKNLENGYFG
                     VIYLIDEIHLEFNSLESKNIDIEVMIEVSQQRKQRKHIIGTSQVYGRLAKPFREQIRN
                     VVLCSNFCKILQINTLIDGSKSVEKDGKLITESVKRFYWFHDPALYDSYDTYAKMKRY
                     KKEWQGRRLT"
     gene            5592..5726
                     /locus_tag="ACZPCL_10640"
     CDS             5592..5726
                     /locus_tag="ACZPCL_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162959.1"
                     /translation="MVQEAINLFADVVAAAIPYAVVFALGQRLVTMFLGMAFKGHVEL
                     "
     gene            5789..>6357
                     /locus_tag="ACZPCL_10645"
     CDS             5789..>6357
                     /locus_tag="ACZPCL_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162960.1"
                     /translation="MYADEYTLTEESYSHDPARSSASAVDLNSWLATVPDDMDFDTYQ
                     EIMAMMHPELSEDYEEDELLDEDGEDVTVASSSTAKRSRSVTYNNVSVQSSYVPDNDT
                     INATYLQYFKDMLVKLPYGTKYVFFRVNDYEYRLVYGKELTYENGVFTGSDMNYIRYY
                     RQSSGNYNYDWLLSSGSEGDFKLTPNGYMV"
BASE COUNT         1801 a         1176 c         1470 g         1910 t
ORIGIN      
        1 ttggtgtgga cacagatact ttcatgaaag ctttccagac catcagcgat taccttccag
       61 agagtgtaac ttcctttaaa gttacctttg gcgctcctca aaaagcaggt ggttacacag
      121 taattgccgc tgttgttgat ccaaattacg aggctgctgt tggaattggc tctttgatca
      181 ttctgccaaa gacagaaaat atagagttgc gttggactca ggatttctct gtattaagtt
      241 acaaagatat tcctctgaat gatgacggtt ctctggatga agaacaggcc atgaagctgt
      301 tcggcgcggt tctgatggat aaagaaactg aaaccgttct tgatagtagt aaagtgcgct
      361 atttgtttgt tggcaaagat ggtgataaag aattctatcg cagcaacaaa ccatctgcaa
      421 agcctggtgt atacacagaa accgcctatg tattaaacgg aaataacatg gattggccta
      481 ttgtccgtac ctatgtgatc ggacgcacag ctgcacttat tcagtttaaa gacggacaaa
      541 tggaaactgc gccaaaagaa gataaggaat acggaacttc cgaggcttta tttgcaacaa
      601 aacatgttac ttacaatggg gaagcacagg agtttaaagc cggcctgtac cttgctgcaa
      661 ctccggaaac agaaatagaa gatgcggaca tccagtatcg ttattacgat gttgtgacag
      721 gaacttacct ggaaggactt cctgtaaatg caggtattta tcgtgttact gctgatttta
      781 aaggaaatgg acagtataca gatgctgcag accagactgg ttatctgatc attgaaaagg
      841 cccagcctaa atttgtgctt gaagaacagc agaattttac atacaatggc gaagctcaaa
      901 agatcatggt tcagtctgta gaagatcagg atcctgaaac aaagtattct gttctgtata
      961 aaaatgacca gacagacgaa aatgcttccc tggaagctcc cgttcatgca ggaacctatc
     1021 gggctatctt agatctgcca gaaacagcaa attatgctgc tgcccatgca gaagtgatca
     1081 tgaacatcat tcctgcaaag gcaaccatcg agatccatcc ggtttctttc agctttgatg
     1141 aacagaccca tggaggatcc tatttggtat atggtattag agatgaaatt cctgagcatg
     1201 aagtaagcta cagtacagat aatgaaatat ttgtggcaga cgagccaatg gcagtaggta
     1261 cttatgcaat gcgaattcag atcacagatc ctgactatga aaatgatgta gaaacacagg
     1321 aagatgcagt tgtcatcaag gaaaaggaaa ttgttcctgt aattgcagaa gcgtgccttg
     1381 atattcaata tgtatgtaat ggtcagatcg taggacatca gctcgtaaaa gtagaaggaa
     1441 attccggtga tctctgcctg tttacagatg aaaatgtagt gttggaaatt cctgaaggct
     1501 atgagaaaga agctggtttc ggaagtattg aagttcctta tggcgaaaca gcgcagacga
     1561 ttgtttctgt aaagctcaaa gaaacccagg atgataatga aaaacctggt gataacggtg
     1621 acaacgacaa cagcggagat aatgacgata acggaagcaa tgacaataac ggaaatagcg
     1681 gtaacggagg taatggcgga tccagttcat ctagtgatgg ttcttcctct tccagccgta
     1741 agaccattat ttccgaaagc ggaagctggc gtcaggatga aaagggtatc tggtttgaaa
     1801 gagcagctag cggatatccg aaaaactgtt ggagttttat tgatggaaaa tggttccatt
     1861 ttaatgcaga aggatactta acacttggct ggtacctctc agaagacgga aagtggtact
     1921 attgtgctcc agacggaaca ttgggcacac cgatcggagc tatgtatacc ggatggctta
     1981 aggatggagc agatggttac tggtattatc tggatccaaa cacaggagag atgttaacag
     2041 gctggagaga gatcaaggga cagtggtact atctcaaccc ggaaactctt ggtgacagtg
     2101 gctggatgca ggattcttca acaaagcagt ggaattataa tgtgaaagcc gttatgccaa
     2161 aaggcgcact gtatgtcaac acacagacac cagatggtta tttcgtagat gaaaatggtg
     2221 ttcgcagata atttctaaat taaaaggcaa tgcagagtca gatgtttttc cggttctgca
     2281 ttgccttttt taattttacg agtaatttgc ctttgggcgc tgattttagg gggtgatccc
     2341 ctgattttct cttaatttaa ccgcattacg ggcgctgcgt cgacgctcgt cttcaagagc
     2401 agcataatgc tttttagtgg tattaacatc ggaatggcca agaacatctg ctaccagata
     2461 tatatcaccg gtttcccggt aaaggttagt accataggtg ctgcgaagct tatgcggagt
     2521 gatctttttt aacggcgtaa cgatctttgc atatttctta actaaattct ccacgctgcg
     2581 cacattcata cgttttttct ggagagagag gaagagagca ttttcactgc cctcttcggg
     2641 gataatgtca tttcgttcat ccagatagtc ctgtagcgcc gtttctactt ccgtaccaaa
     2701 gtaaacagta acttctttcc cgcctttccg gtgaatatgg atcccaccat ttttgaaatc
     2761 aatgtcattt atgtctaatc ctacacattc agaaacacgg atgccggttc cgagaagcaa
     2821 tgtgagaaga gcgagatcgc gcagtttagt ccggttgtga aacgctttct gcttatcagt
     2881 cagaccatcc ccttgttcca ctgcatccag aagaagagca acttcatcaa tatcaagacg
     2941 gatgatttct ttttcatgaa gtttgggaag ctggaccaaa gcagcaggat ttgttttcag
     3001 tttttctgta cggaaaaagt agttataaaa gcttttaagg gaagagatct tgcgcataat
     3061 tccccgttct ttattaacaa tttcctgatt gtgctcatta aagcgatatt ttaaatattc
     3121 catatattct tccagatctg ttaccgtgat ctgatccaaa tgatctaatg taaaatccat
     3181 tcgttccatt ttggaaaaaa ccggattttc ctttttgagg aaatcgaaaa aaacagagag
     3241 atcataagca taggcaatcc gggtcctggt agaagttcgt ggttcgatcc cacgaaaaaa
     3301 ttctgtacag aagggtggga gagtcttcgt catttcccgc agctttttta tattctcaat
     3361 atctttttgt tcgtaatagg gtagtgtcgt tgccataatt atgtatgcct catttataat
     3421 ttatatataa taaatatttt cagattattt tgtattatta tttagaaagc ctgggccagt
     3481 cttcaaaatt tccgttaatg accgcatgaa ataaacgttc acggacaccg ggactgtcca
     3541 tattcagtgt ccggatcaga tttttcacat attcacggtg tgtatgtcca tccataggac
     3601 atggattttt acagactgga agctgatatt tattccggaa accttttaca tccgcttcgg
     3661 agaccagcat caaaggccgg ataaccgtta aatcggtacg atccaaatgg gtaaccggtg
     3721 gaaaggcata aaatcgtccc tcatataaaa gcgacattaa ggcagtttcg atcaggtcgt
     3781 ccttatggtg ggcataagca attttgttgc agccaagttc caaagctttc tggtttaggg
     3841 caccttttct cattttcgca cacaaagcgc atggattaga ctctttacgg gcttcaaaca
     3901 ggatctttcc aatttctgtg gagatgatct catagggaac attaaaatct gcgcaaaggg
     3961 cctttatagg ctctaaattc atagtatcta agcccatatc tacagtaatg gcagacaggg
     4021 tgaaatgctt cggataaaat ttctgaagct gtgacagacc ataaagtaaa gtaagactgt
     4081 ctttaccacc ggaaatacca atggcaatat gatcaccgtt ttcaatcatc tggtattggt
     4141 caatagcctg gcggagaagg cttaagagac gttggagttt catttgagag tgggtccttt
     4201 ctgaacgaga attaataaag atgattttgt tttagcatgt acggattata ttaattaaaa
     4261 taaatataga aatttcagaa aaatatagag acattaaggt atggagagtg atattcaact
     4321 attcgcgaaa caatacttta acgaatagtt gaaccgattt cacattatat tcttaataaa
     4381 ccacttacat acatcccaat attttcaatt atttccacat atattttatt tcatttcatc
     4441 tacgcataaa ctgactttcg cgaatagttg cgaatcactc tctttcctcg tgttatcggg
     4501 ctttttggct tgatataaaa aatattatag gaggtttttg catgaaattc aaggaatctt
     4561 taaaaaagta cggttgcctg cctgttgcta ctggtttggc ttccgtgggg ctggctgttc
     4621 cggcttttgc tgagggtgcc gctccaactg ttgtgactgc atctgattgg tcaagtgtta
     4681 tttctgcgtt gactgctcag atctctgtaa gtacgatcat tgcagcactt gctacttttg
     4741 tggctgctgg tatcggtatc gtatttatgt ggtggggact tcgtaaaggt atccgctccc
     4801 ttatggcagc tttccgtaaa ggccgcatgt ccatttaatt tttgtttgtc tcctgtagtg
     4861 agggcttgcc ggtttggtaa gtcctctttt gtttaggtgg tgtttatgga acttaattta
     4921 ttagatggct cgttaaatcc tgttaatatc gggcgtgtca ttaaacataa ccatgacttc
     4981 tataagtcgc atcccggcta cttccgtccg gaggggctgt tggtgttctg cggtgaacag
     5041 ggttctggta aaacgttatc ggcggtgcaa tatgttaaga aactttgtag tgaatatcct
     5101 gatgctattt tatgtaccaa tgttcaaatt tctgggctta atcctagcac acatgtagtt
     5161 gagtatgacg gcctggactg cctgaaaaat cttgaaaatg gctactttgg tgtgatctat
     5221 ctgattgatg aaatccactt ggagtttaac tctctcgaat cgaaaaatat cgatattgag
     5281 gtaatgattg aggttagtca gcagcggaaa caacggaaac acattatagg tactagccag
     5341 gtctatggcc gtcttgctaa acctttccgg gagcagatca gaaacgtggt gctttgctcc
     5401 aacttctgca agatcttgca gatcaatact cttattgatg gtagtaagtc ggttgagaaa
     5461 gacggtaaac tgatcacgga atcagttaag cggttctact ggtttcatga tccggctttg
     5521 tatgacagtt atgatacgta cgcaaaaatg aagcgttata aaaaagaatg gcaaggaagg
     5581 aggcttactt gatggttcag gaagcgatca atttatttgc tgatgtggtg gcagctgcta
     5641 tcccttatgc agttgtgttt gcgctgggtc agcggcttgt tactatgttc ttgggtatgg
     5701 cttttaaggg gcatgttgaa ttatgataag gcggtggaaa aatgttatat tatgcagttt
     5761 gtttctttgc gttgtggggg ctgtgtcggt gtatgcggat gagtacacgc ttactgagga
     5821 aagctattcg catgatccgg cgcgttcttc tgcttcggcg gtggatttaa atagctggct
     5881 ggctacagta ccggatgata tggactttga tacatatcaa gaaattatgg ctatgatgca
     5941 tccagaactt tctgaggatt atgaagagga tgagctgctg gatgaggacg gtgaggatgt
     6001 gacggttgct tcctcttcta ctgctaagcg ttcccggtct gttacgtata acaatgtttc
     6061 ggtacagagc agctatgtgc cggataatga tacgattaac gcaacgtatt tacagtattt
     6121 caaggatatg cttgttaagc tgccctatgg cacgaaatat gtatttttca gggtcaatga
     6181 ctatgagtat cggcttgtgt atggtaaaga gctgacctat gaaaatggtg tgtttaccgg
     6241 ttcggatatg aattatatcc gttattatcg tcagagttcc ggcaattata attatgattg
     6301 gctgctctct tctggctctg agggtgattt taagcttacg cctaatggtt acatggt
//
LOCUS       DBMDUA010000158         6313 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5049_length_6313_cov_3.115422, whole genome shotgun sequence.
ACCESSION   DBMDUA010000158 DBMDUA010000000
VERSION     DBMDUA010000158.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6313)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5049_length_6313_cov_3.115422"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /gene="prfB"
                     /locus_tag="ACZPCL_10650"
     CDS             <1..806
                     /gene="prfB"
                     /locus_tag="ACZPCL_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006906636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR1162961.1"
                     /translation="MRMKLLLSGEYDGYDAILRLNAGAGGTESCDWCSMLYRMYCRWA
                     EKKGFKVEVLDYLDGDEAGIKSVTIQISGENAYGYLRSEHGVHRLVRISPFNAAGKRQ
                     TSFVSCDVMPDIKEDIDIEINPDDIRIDTYRSSGAGGQHINKTSSAIRITHYPTGIVV
                     TCQNERSQFQNKDKAMQMLKAKLYMLRQEEQAAKAAGIRGDVKDNGWGSQIRSYVLQP
                     YTMVKDHRTGEESGNVDAVLDGSIDNFISAYLRWMSLGCPNKNAMSDDQ"
     gene            1026..1469
                     /locus_tag="ACZPCL_10655"
     CDS             1026..1469
                     /locus_tag="ACZPCL_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1162962.1"
                     /translation="MEDKSKYFVVKQKAVPEVLLKVVEAKKLLETVRAITVQEAADRV
                     GISRSSFYKYKDDIFPFYDNTKGKTITLVVQMDDRQGLLSDLLHVVAVYRANILTIHQ
                     SIPVNGVATLTLSVEVREDTGNVSSMIDELEVLDGIHYVKILARE"
     gene            1525..2766
                     /locus_tag="ACZPCL_10660"
     CDS             1525..2766
                     /locus_tag="ACZPCL_10660"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR1162963.1"
                     /translation="MIKTAVMGYGTIGSGVAEILDQNKAEVAKSAGQEVELKYVLDLR
                     DFPDSPVADKIIHDFKIIEEDPEVQVVVETMGGLNPAYPFVKACLLAGKHVVTSNKAL
                     VAAYGTELLAIAKEKQVNFFFEASVGGGIPIIRPLYRCLMGERIEEITGILNGTTNYI
                     LTKMDKEGETFENALKEAQNLGYAERNPEADVEGHDTCRKIAILTSMATGKTVNYEDI
                     YTEGITKITDIDFKYAEKMGTSIKLFGTSRIKDGKVSAYVAPVMISKNNPLYSVNDVF
                     NGIVVKGNMLGTSMFYGSGAGKLPTASAVVADIIEAVQNIDKNVPLGWTDEKQEILPM
                     DQTKFRYFIRVAGSYRNKEQELNEYFGKAEVIELYGMDEFAILTKEMTEGELKAAASA
                     FDTKAQSRSEYGIKQMIRAVL"
     gene            complement(2872..3282)
                     /locus_tag="ACZPCL_10665"
     CDS             complement(2872..3282)
                     /locus_tag="ACZPCL_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HIR1162964.1"
                     /translation="MNNTSKKPRKTSSSAEQLAACSLISGIAGIICSLFYLPFSLVSG
                     KNTPMGLICGVIGIVLAIMAKNADIRPGKKYPGKALAGFIFSVIAIALTMFFFYSLIN
                     YYDLLRDPVKGPQINQLIYQMQQQLQMQMQAPAN"
     gene            3424..5580
                     /locus_tag="ACZPCL_10670"
     CDS             3424..5580
                     /locus_tag="ACZPCL_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HIR1162965.1"
                     /translation="MDIIKALAEELNIGRHQAEAAVKLIDEGNTIPFIARYRKEATGS
                     LNDEVLRNLDERLKYLRNLEDRKEQVISAISDQGKMTAELEKQIREAATLVAVEDLYR
                     PYKPKRRTRAMIAREKGLEPLANVILLQNLKEPVEKAAEAYISVEKGVESVQDAINGA
                     MDILAEGISDDAKYRTYIRNITMNKGLLQSTAKDGEKQSVYETYYNYEEPVKKAAGHR
                     ILALNRGEDEKILTVKVAAPTEQILGYLEKQVITKENPYTTPVLKAVIQDSYSRLIAP
                     SVDREIRSELTDKAQEGAIRVFGKNLEQLLMQPPIAGKVVLGWDPAFRTGCKLAVVDS
                     TGKVLDTVVIFPTAPQNKVAESKKILKDLIHKYGISLISVGNGTASRESEQIIVELLK
                     ELKEQVQYVIVNEAGASVYSASKLATEEFPKFDVGQRSAVSIARRLQDPLAELVKIDP
                     KSIGVGQYQHDMNQKNLGEALGGVVEDCVNKVGVDLNTASASLLEYVSGINKTLAKNI
                     VAYREENGAFKNRKQLLKVAKLGPKAFEQCAGFMRITGGDNPLDGTSVHPESYEAAGK
                     LLEKLGYTEDQLSHGGLNGISKKIGDYKKMAQEMGVGEITLRDITAELEKPARDPRDE
                     MPRPVLRSDIMEMKDLKPGMVLKGTVRNVIDFGAFVDIGVHQDGLVHISQMTDRYIKH
                     PLEAVSVGDVVDVKILSVDMDKKRIALTMKGVKDGN"
     gene            5570..5995
                     /gene="tsaE"
                     /locus_tag="ACZPCL_10675"
     CDS             5570..5995
                     /gene="tsaE"
                     /locus_tag="ACZPCL_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR1162966.1"
                     /translation="MVIDTWGPEETRAFGKKMGQEARPGQVICLNGDLGVGKTIFTQG
                     FAEGLGIDEPVNSPTFTIVQQYDTGRLPLYHFDVYRIGDISEMDEIGYEDCFYGDGVS
                     LIEWSQLIEEILPEHVTEITIEKDLEKGFDYRKIRVEEK"
     gene            5995..>6313
                     /gene="tsaB"
                     /locus_tag="ACZPCL_10680"
     CDS             5995..>6313
                     /gene="tsaB"
                     /locus_tag="ACZPCL_10680"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR1162967.1"
                     /translation="MKVLGIESSSLVASVALVTDDILTAEYTVNFKKTHSQTLLPMLD
                     EIVKMLGLDLETIDAIAAAGGPGSFTGLRIGAATAKGLGLALKKSLIHVPTVDAIAYN
                     MWGA"
BASE COUNT         1986 a         1192 c         1583 g         1552 t
ORIGIN      
        1 agatgcgtat gaagcttctg ctttccggcg aatacgatgg ctatgatgcc attttacgtc
       61 tgaatgcagg tgcaggcgga acagagtcct gtgactggtg cagcatgctg taccgtatgt
      121 actgccgttg ggctgagaaa aagggcttta aggtagaagt ccttgactat ctggacggtg
      181 atgaagcagg tatcaaatct gttaccattc agatcagcgg ggaaaatgcc tatggctatc
      241 tccgttccga gcacggtgta caccgtctgg tacgtatttc cccgtttaat gcagcaggca
      301 agagacagac ttctttcgtg tcctgtgatg tgatgccgga tattaaagaa gacattgata
      361 ttgagatcaa tccggacgat atccgtattg atacctaccg ttccagtggt gccggtggac
      421 agcatatcaa taagacctct tctgcgatcc gtattaccca ttatcctaca ggtatcgttg
      481 ttacctgcca gaatgagcgt tcccagttcc agaataagga caaggcaatg cagatgttaa
      541 aggcaaagct gtatatgctt cgccaggagg aacaggcagc aaaggcagcc ggtatccgtg
      601 gtgacgtaaa ggacaatggc tggggaagcc agatccgctc ttatgtcctt cagccttata
      661 ctatggtaaa agaccatcgt acaggggaag aaagcggaaa tgtagatgct gttttagatg
      721 gctctattga caactttatc agtgcttatc tgcgctggat gagtcttggc tgtccgaata
      781 aaaatgccat gtcagacgat caataagcat acgcataata aatttacaaa gactgctgta
      841 agacgaaaag ctattttaca gcagtctttt ttatgtgtta tagatggatc tgaaatattg
      901 tatactttgt tcttaattta acataagaaa gtggttgcaa ttcccataaa agtatgttaa
      961 tatcttcctc atgaatacag atgtgttcgc gatacggaca tggacaggaa ggaagaaaag
     1021 ggttcatgga ggacaaaagt aagtattttg ttgtaaaaca aaaggcagtg ccggaagtac
     1081 ttttaaaagt cgtagaggca aagaaacttc tggaaacagt aagagccatt actgtgcagg
     1141 aagctgcaga ccgggtaggg atcagtcgca gttcttttta taaatataag gacgatattt
     1201 ttcctttcta tgataacaca aagggaaaaa cgattacact ggtagttcag atggatgaca
     1261 gacagggact tttaagtgac ctgcttcatg tggttgcagt gtaccgggca aatatcctta
     1321 ccatccatca gagtattccg gtcaacggag tcgctacttt gacactgagt gtggaagtaa
     1381 gggaagatac gggaaatgtt tccagtatga tcgatgaact ggaagtatta gacggtatcc
     1441 attatgtaaa gatactggcc agagaataaa aacaaagatc gggccggtaa aaaatatcag
     1501 gtagcataca gggagcgaga gattatgatc aagacagcag ttatgggata cggtaccatt
     1561 ggatctggtg ttgcagagat cctcgaccag aataaggcag aagttgcaaa aagtgcagga
     1621 caggaagttg aattaaaata tgttctggat ctgagagatt ttccggacag cccggtagca
     1681 gataagatca ttcatgattt taagatcatt gaagaagatc cggaagtaca ggtagtagtg
     1741 gagactatgg gcggattaaa tccggcatac ccatttgtaa aggcctgcct gttggctgga
     1801 aaacatgtag ttacttccaa taaggcttta gtagctgctt atggaacaga gctgcttgcg
     1861 attgcaaaag aaaaacaggt aaacttcttc ttcgaggcaa gtgtaggcgg cggaattccc
     1921 atcatccgtc cattataccg ctgcctgatg ggcgagcgca ttgaagaaat cacaggtatc
     1981 ttaaacggaa ccaccaacta tatcctgact aagatggata aagagggcga aacttttgaa
     2041 aatgccttaa aagaggctca gaacctggga tatgcagagc gcaatccgga agcagacgta
     2101 gaaggccatg atacctgccg taagatcgcg atcttaactt ccatggcaac gggaaagact
     2161 gttaattacg aagatattta tacagaaggt attaccaaaa ttacagatat cgattttaag
     2221 tatgcagaga aaatgggaac ctccattaag ctgtttggaa ccagccgcat caaggatggc
     2281 aaggtaagcg cttatgtagc tcctgttatg atcagtaaga acaatccgtt atactctgta
     2341 aatgatgtat ttaacggcat cgttgtaaag ggaaatatgc tgggaacttc tatgttttac
     2401 ggaagtggcg caggaaaact gccaacagcc agcgcggtag ttgcagatat tatcgaagca
     2461 gtacagaata tagacaaaaa tgttccactt ggctggactg atgaaaaaca ggagatcctt
     2521 cctatggatc agaccaagtt ccgctacttt atccgtgttg caggctctta cagaaacaaa
     2581 gagcaggagt taaatgagta tttcggcaag gccgaggtga tcgaactcta cggaatggat
     2641 gaatttgcga tccttacaaa agaaatgaca gagggagaat taaaggcagc tgcaagcgcc
     2701 tttgatacaa aagcccagtc acgttctgaa tatggtatca agcagatgat ccgtgcggtg
     2761 ctgtaattca tacttcaata aataaacagg taaaataata aaagccattc tcttaatata
     2821 aaaacgagag aatggctttt tgcgtgttca atattaattt tatgaaacaa actagtttgc
     2881 cggtgcctgc atctgcatct gaagctgttg ctgcatctgg tagatcaact ggttgatctg
     2941 cggtcccttt accgggtcac ggagcagatc ataataattg atcaggctgt aaaagaagaa
     3001 catggtaaga gcgatcgcga tcacactgaa gataaaacca gccagtgcct tacctggata
     3061 tttctttccc ggacggatgt ccgcgttttt tgccataatg gcaagcacga taccgattac
     3121 tccgcagatc agtcccatag gagtgttctt tccgcttaca agggaaaatg gcagataaaa
     3181 aaggctgcag ataatacctg caataccgct gatcagggag caggctgcca gctgttccgc
     3241 tgagctggat gtttttcttg gttttttgga agtattattc ataagttaaa gtaggatcct
     3301 ttctatttcg cttattatag catgaaaaac aatcactgtc cagacaaggg caagtcttgc
     3361 ataaggtccg cggatgaatt ataataaaac ctcagaacga caaaaaatat gtgaggacta
     3421 tcaatggata ttattaaagc actggcagaa gaactgaata ttggacgcca ccaggcagag
     3481 gcagctgtaa agctcattga cgaaggcaat accattccgt tcattgccag ataccgtaaa
     3541 gaagcaaccg gttcattaaa tgatgaagtt ttaagaaatt tagatgaacg tttaaaatat
     3601 ttaagaaacc tggaagacag gaaagagcag gtcatcagtg ctatttctga ccagggaaaa
     3661 atgacagcag aactggaaaa acagatccgt gaagcggcaa ctttagtggc agtagaagac
     3721 ttatatcgtc catacaagcc aaaacgccgt accagggcta tgatcgcccg tgaaaagggc
     3781 ttagagcctt tggcaaatgt gatcctttta cagaatttaa aagaacctgt ggaaaaagcg
     3841 gcagaggcct atatttctgt tgaaaaaggc gtagaaagtg ttcaggatgc cattaacggt
     3901 gccatggaca ttcttgcaga aggcatttct gacgatgcaa aataccgcac ctatatcaga
     3961 aacattacca tgaacaaggg acttctccag tcaactgcaa aggatgggga aaagcagtct
     4021 gtttatgaga catactataa ttacgaagaa ccggtgaaaa aagcagccgg acaccgcatc
     4081 cttgcattaa acagaggaga agatgagaag atccttactg taaaggtggc agcgccaaca
     4141 gagcagatct taggatacct ggaaaaacag gtgatcacaa aggaaaatcc atataccaca
     4201 cctgtattaa aggcagtgat ccaggacagc tacagccgtc ttattgctcc ttctgtagat
     4261 cgtgagatcc gcagtgaact gactgataag gcacaggaag gtgctatccg tgtatttgga
     4321 aagaacttag agcagcttct tatgcagcct ccaattgctg gtaaagtagt attaggctgg
     4381 gacccggcat tccgtaccgg ctgtaagctg gctgtagtgg acagtaccgg caaagtatta
     4441 gatactgtag ttattttccc aactgcccca cagaacaagg tggcagagtc caagaaaata
     4501 ttaaaagacc tgatccataa atatggcatt tccctgatct ctgtgggaaa cggaactgct
     4561 tccagagagt ctgaacagat catcgtagag ctgttaaagg aattaaaaga gcaggtacag
     4621 tatgtgatcg taaatgaggc cggtgcttct gtttattctg caagcaagct ggcaacagaa
     4681 gaattcccga agttcgatgt aggacagaga agcgcagttt ctattgcaag aagacttcag
     4741 gacccactgg ctgaactggt aaagatcgat ccaaaatcca ttggtgtagg ccagtatcag
     4801 catgatatga accagaagaa cctgggagaa gcattgggcg gcgttgtaga agactgtgta
     4861 aataaagtag gcgttgattt aaatacagca tctgcttccc tgttagaata tgtttccggt
     4921 atcaacaaga ctcttgcaaa gaacattgtg gcatacagag aggaaaatgg tgcttttaag
     4981 aaccgtaagc agctgttaaa ggtagcaaaa ctgggtccaa aggcatttga acagtgtgcc
     5041 ggatttatga ggatcacagg aggcgataac ccattagacg gtaccagcgt tcatccggaa
     5101 agctatgagg cagcaggaaa gctgttggag aaattgggat acacagaaga tcagctgtcc
     5161 catggcggtt taaatggtat cagcaaaaag attggtgatt ataagaaaat ggcccaggaa
     5221 atgggcgtag gtgagatcac acttcgtgat attaccgctg aactggaaaa accggccaga
     5281 gacccgcgag atgagatgcc gcgtcctgtt ttaagaagtg acattatgga aatgaaagac
     5341 ttaaagccgg gaatggtatt aaagggaacc gtgcgaaatg tcattgactt tggtgcgttt
     5401 gtggatatcg gcgttcatca ggacggcctg gtacacattt cccagatgac agaccgttat
     5461 attaagcatc cattagaggc agtaagcgta ggcgacgtgg tagatgtgaa gatcttaagc
     5521 gtagatatgg acaagaagag gatcgctctt acaatgaagg gagtaaagga tggtaattga
     5581 tacttggggt ccggaggaaa caagggcttt tggaaagaaa atgggacagg aagcaagacc
     5641 gggacaggtg atctgcttaa acggtgacct gggagtggga aagactatct tcactcaggg
     5701 atttgcagag ggactgggga tcgatgagcc ggtaaacagc ccgacattta ccattgtcca
     5761 gcagtatgat acaggaagac tgccgcttta tcattttgac gtataccgta tcggtgatat
     5821 cagcgaaatg gacgagatcg gatacgaaga ctgtttctac ggagacggcg tttctctcat
     5881 cgaatggtcc cagctgattg aggagatcct tccggaacat gtgacagaga tcaccattga
     5941 aaaagacctg gaaaaaggat ttgactacag aaagatacgg gtggaggaaa aataatgaaa
     6001 gttcttggta ttgaaagttc atctttagta gccagtgtgg cacttgtaac agatgacatt
     6061 ctcacagcag aatatacagt aaactttaaa aagacccatt cccagacact ccttcccatg
     6121 ttagatgaga tcgtaaaaat gctgggactg gacctggaga ctattgatgc cattgcagca
     6181 gcagggggcc cgggctcctt taccggtctt cgtattggcg cagctactgc aaaaggactg
     6241 ggactggcat taaagaagtc tctgatccat gtacctacag tagatgctat tgcttacaat
     6301 atgtgggggg cat
//
LOCUS       DBMDUA010000159         6270 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5079_length_6270_cov_3.441887, whole genome shotgun sequence.
ACCESSION   DBMDUA010000159 DBMDUA010000000
VERSION     DBMDUA010000159.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5079_length_6270_cov_3.441887"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="ACZPCL_10685"
     CDS             <1..462
                     /locus_tag="ACZPCL_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162968.1"
                     /translation="EREVLKLSGLYQKTSEHAALELETVVLNINIGYNRELMERCKEL
                     HDYAEFVGRVRENLKSKNPLLHAVNEAVEYCIRNDILAEFLKKNRSKVIKMSIYEYDA
                     EKVQRQFREQWKQMGLEEGRAEGRQTGIKIFAYILQLSMLSWSFSCICRCG"
     gene            complement(679..1632)
                     /locus_tag="ACZPCL_10690"
     CDS             complement(679..1632)
                     /locus_tag="ACZPCL_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HIR1162969.1"
                     /translation="MKLFPPLKEISITEIEGLRIGNAQDLDAMTGVTVLRFDEGAKAG
                     CDISGGGPASRETPLTSPVTADNPINAIVFSGGSAFGLAASDGVMRYLEEHGIGYDTG
                     FAKVPLVCQSCIYDLGIGRADVRPDAGMGYAACEDAEKNQPKCGIIGAGTGASVGKIC
                     GREYAAKTGLGIYAVSAGNLKVAAVVVLNALGDIYDPSTGEKIAGPKLPGMDGFADSR
                     KELYKLSQQTDLFSFKNSNTTIGAIITNGDFSKAELNKIASMTRSAYSRCINPVGTMA
                     DGDSIYVASCGPRVVADVNMAGTLAAEVMAEAIQIAVRTAK"
     gene            complement(1718..2350)
                     /locus_tag="ACZPCL_10695"
     CDS             complement(1718..2350)
                     /locus_tag="ACZPCL_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1162970.1"
                     /translation="MKTTTANPVTSEMSRSRTTFMVKLALMVAIIFVMAFSPLGYLRT
                     PGLTITFLTVPVAVGAIILGPTAGAICGGAFGLTSAIMALTGGSAFSAALLQINPFGL
                     LFTLLVPRILEGWLCGLIFAALKKVSKNGAFVIASLSCPLLNTLLFMSSLVLFFFHTE
                     YIQNIASGLGTSNPLFFVVLFVGIQGAIEAVVCTILGSAVSRALTAALKR"
     gene            2897..4282
                     /gene="murC"
                     /locus_tag="ACZPCL_10700"
     CDS             2897..4282
                     /gene="murC"
                     /locus_tag="ACZPCL_10700"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR1162971.1"
                     /translation="MYQIDFTTPIHIHFIGIGGISMSGLAEILLEEGFTITGSDSKES
                     PLTRHLEEKGARIYYGQKASNIEAEPGIQVVVYTAAIHPDNPEFKAAKDKNIPMLTRA
                     ELLGELMRNYKESVAVSGTHGKTTTTSMITDILLAADTDPTISVGGILKDIGGNIRVG
                     GPDLFVTEACEYTNSFLSFYPTIEVILNIEEDHLDFFKDINDIRHSFRLFAEKLPKGG
                     LLVINSSIENINEITSGLPCRIVTFGKDLSSMYTAQNITYDEFARPSYDLVVQGEIVN
                     RITLGVTGEHNVYNSLAAIAVAMELGIGFDAIMSGLKNFKGTDRRFEKKGEIGGVTII
                     DDYAHHPQEIAATLSAAKNYPHKKLWCVFQPHTYTRTKAFLDEFAQALSAADEVILAD
                     IYAARETDTLGVSSADIASRIEKLGTPAHYIPSFDEIETFILEHCMQGDVLITMGAGD
                     IVRVGEKLLGE"
     gene            4557..5636
                     /locus_tag="ACZPCL_10705"
     CDS             4557..5636
                     /locus_tag="ACZPCL_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HIR1162972.1"
                     /translation="MSRIFKDRLSTGVTPVADAFIDNYMAAANGEYVKVYLYILRHQN
                     QEDLTLEQIADALNHTEADVKRAINYWQKAGVLENVQDNTLEGISAEQNRTSWSIDIE
                     VPPAASKETAASQAPVSAPAYSAEQVSRLNKDEEFSQLLYIAQKFLNKAFTPRDCQVF
                     AYLYDTLGMNIGLLEYLAEYCAQNGHTSVRYLETVALNWHEKGIRTAEEAQEYSTAYT
                     KDAFAVMKAFGLNSRKPAVPEQKIMEKWFKDYGFDRELVLEACSRTINAIHTPSFQYA
                     DSILTDWKKAGVKTLADVKGMDARRAERAQNGAVKRLQSYGNGAAAQNNRKTSQNQFH
                     NFKQRDTDYDALMLQNVKEWMDSPE"
     gene            5652..>6270
                     /locus_tag="ACZPCL_10710"
     CDS             5652..>6270
                     /locus_tag="ACZPCL_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1162973.1"
                     /translation="MALSNSQYDAIMREYGKQQMENQHSQEARKKEIYQKVPAVKELE
                     AEIAERSVACARKLLEGNQEAIYELREKLADLKEQKALLIKAAGYSDDYLELHYRCKD
                     CRDTGWVDGHKCHCFLRAQMKLLYAQSNLERVLEQENFDHLTFQYYDNSQIIPEIGMT
                     NADYMRRVVKSCMEFAQNFDSHQDNLLFTGSTGVGKTFLTNCIARK"
BASE COUNT         1955 a         1328 c         1430 g         1557 t
ORIGIN      
        1 gagcgggaag tgctgaaact ttctgggttg taccagaaaa catcagaaca tgcagctttg
       61 gaattggaaa cagtagtgct gaacataaat atcggttata acagagaatt gatggaacgt
      121 tgcaaggaac ttcatgacta tgcagaattt gtaggccggg taagagaaaa tctcaaatcg
      181 aagaatccac tgcttcatgc tgtaaatgaa gcggtagagt actgtatacg aaatgatatc
      241 cttgcggaat ttttaaagaa aaatcggtcg aaggttatta aaatgagtat ttatgagtac
      301 gatgcggaaa aagtacagag gcaattcagg gaacagtgga aacagatggg attggaggaa
      361 ggccgcgcag aaggccgaca gacaggcatt aaaatttttg cctatatttt gcagttgagc
      421 atgctttctt ggagtttttc ctgcatctgc cgctgcggct gattttttgt tagttgtata
      481 cggatttcag aggcaaacgc aacgttaact tccttggaat tttagcacaa ctgacaaaaa
      541 atgttcctac aaaatcaaaa cagaaagatt tagagagaac attaaggtaa aaacatcccc
      601 catctgtggt ctgcggatgg aggatgttgt aaggtgtttg tttaaaaccg ccgttggcgg
      661 tttttgtttt atcaatattt attttgcagt tcttacagct atctggatgg cttctgccat
      721 tacctccgca gccagcgttc ccgccatatt tacatcagct acgactctgg ggccacagga
      781 ggctacatag atagaatcac cgtctgccat agttcctaca ggattgatgc agcgggaata
      841 ggcacttcgg gtcatagatg cgatcttatt taattccgct ttggagaaat ctccatttgt
      901 gataatggca ccaatggtag tgttggaatt tttgaaagag aaaagatctg tctgctgact
      961 cagcttataa agctcttttc tggagtctgc aaatccatcc ataccaggaa gtttcggtcc
     1021 tgcgatcttt tctccggtag atggatcata aatatcgcct aaagcattta aaacaacaac
     1081 agcggcaact ttaagattac cagcactgac tgcatagatg ccaagaccgg tttttgcggc
     1141 atattcccgt ccacagatct ttcctacact ggctccagtt cctgcaccga taatgccgca
     1201 tttgggctgg ttcttttccg catcttcaca tgcagcatat cccatgccgg catccggccg
     1261 cacatctgcc cgaccgatcc caaggtcata gatacaggac tggcatacta acggcacttt
     1321 tgcaaagcca gtatcatagc cgatcccatg ttcttccaga tagcgcatta cgccgtcact
     1381 ggctgccaga ccaaaagcag acccaccaga aaaaacaatg gcattaatgg gattatcagc
     1441 agtaacagga gaggtaagag gcgtttctct ggatgccggg ccgccgccgc tgatatcgca
     1501 gccagctttg gcaccttcat caaagcgtaa aacggtaact ccggtcatgg catccagatc
     1561 ctgggcattt ccaatacgaa gcccttcaat ctcagtaatg gaaatttctt ttaaaggtgg
     1621 aaataatttc ataataaata gatctcccta taaatctttg tataatattt ttcccggaca
     1681 aaactgtccg ggaaaatgta cttacacgtt atatttatta tctttttaat gcagctgtca
     1741 gtgctctgga aacagcactt ccaagaatgg tgcatacaac agcttcaata gcaccctgaa
     1801 tacctacaaa caggacaaca aagaacagtg gattggaagt tccaagtccg gaagcaatgt
     1861 tctggatgta ttctgtatgg aagaagaaca gtaccaggga actcataaac agcagggtgt
     1921 ttaacagcgg gcaggaaagg cttgcaataa cgaaagctcc gtttttggaa actttcttaa
     1981 gagcagcgaa gatcaggccg cacagccatc cctccaggat acggggaact aataaagtaa
     2041 ataaaagacc aaatgggttg atctgtaata aagctgcgct gaaagcagaa ccaccggtaa
     2101 gagccataat ggcactggtc agaccaaaag cgccgccgca gatggcgccg gctgttggac
     2161 caaggataat ggcgcctaca gcaacaggca cagttaaaaa ggtaatggta agtcccgggg
     2221 tacgcagata accaagaggt gaaaatgcca tgacaaagat aatggcaacc ataagagcta
     2281 acttcaccat aaaagtggtc cgtgaacggg acatctcaga agttacggga tttgcagttg
     2341 ttgttttcat tgtgttctcc tttttcgctg ataaaacggc actggttcta tctgcatatc
     2401 tgcagtttca cagctccccc tgtttttcag cctttattta gtttgttttg gggatacaat
     2461 gaacggaaac ttaagagtaa aaagctcagt tccagaccat aagatcatag aaaatcaagt
     2521 agaataaagt tgtatacatg atacaggtaa aaagcatctg gttacagcca taaaaggtct
     2581 gcacgggcaa attcctgcaa agtatataat ttataaaaag gtctgatcat gatacatttt
     2641 cctaagaata acgttcaaat ttaatatagc ataaatctgg aaaacagcaa gtttttatgc
     2701 aggtacaaag gaaaaaagca taaagctggc agtacatttg acagaaaatc cagtagcatt
     2761 tcagggtaaa tgtggttata atgtaatggg tgccgaaaaa aacaagcgcc gcgaaagcgg
     2821 catttgtttt tttcaagccc attaacgaac aacccgtctg cgaagcagac agtagagcgc
     2881 gtagaaagga cacaccatgt atcaaataga tttcaccacc ccgatccaca tccatttcat
     2941 cggcatcggc ggtatcagta tgagcggtct tgctgagatc ctcttagaag aaggcttcac
     3001 tattaccggt tccgactcaa aagagtcccc tttaacccgt cacctggaag aaaagggcgc
     3061 cagaatttac tacggccaga aagcctctaa cattgaagcc gagccaggca tccaggttgt
     3121 agtctatact gcagctatcc acccggacaa tccggaattc aaggcagcaa aagacaagaa
     3181 catcccaatg cttaccagag cagaactctt aggcgaatta atgcgcaact acaaagagtc
     3241 tgttgcagtt tccggaaccc atggaaagac aactaccaca tccatgatca cagacatcct
     3301 gttagcagct gatacagacc ctaccatttc cgtaggcggt attttaaaag acatcggcgg
     3361 caacatccgt gtaggcggac cggacctgtt tgtaacagaa gcctgcgagt acaccaacag
     3421 cttcttatcc ttctatccaa ccatagaagt gatcttgaac attgaagaag accacttgga
     3481 cttcttcaaa gacattaacg acatccgtca ttccttccgc ttatttgcag aaaaacttcc
     3541 aaaaggcgga cttttagtca tcaacagcag tatcgaaaac ataaacgaaa tcacgtccgg
     3601 cttaccatgc cgtatcgtaa ccttcggaaa agacctttcc agcatgtata cagcccagaa
     3661 tatcacctat gatgaatttg caagaccttc ctacgacctg gttgtccagg gtgagatcgt
     3721 caaccgcatt accttaggtg taacaggcga gcacaatgta tacaattccc tggcagccat
     3781 tgcagtagct atggaactgg gcattggctt tgacgctatt atgtccggtc ttaaaaactt
     3841 taagggaaca gaccgcagat ttgagaaaaa aggtgagatc ggcggcgtga ccattatcga
     3901 tgattacgca caccatccac aggagattgc ggctacactg tcagcggcta aaaattatcc
     3961 gcacaagaaa ctgtggtgtg tattccagcc acatacctat acccgtacaa aggcattttt
     4021 agacgaattt gcccaggcgc tttccgcagc agatgaagtg atcctggcag acatctatgc
     4081 agccagagag acagatacct taggtgtaag ctctgctgat attgcttcca ggatcgaaaa
     4141 actgggaaca ccagcccact atatcccatc ttttgatgaa attgagacat ttatccttga
     4201 acattgtatg cagggtgatg tgttgataac tatgggggcc ggagatatcg taagagtggg
     4261 agaaaagctc cttggagagt gatgtactct cgatttgtcc acaatatcca cagcgttttc
     4321 aacatttttc agaacgaaaa aatgtggatt tccagattaa cataatataa tgtcgcacga
     4381 tttaaaaaca taaggtttta tgcggagaag acaggtgtat gtcagtgtgg cagcaggaaa
     4441 aacggaaacc gctgtccaca ttatccacaa tatccacatt tgtggatgtg taaaaatgca
     4501 cctgttttct tttttatgtg gatgtgttat gatgaaaaca atgaggagtg gtaaggatga
     4561 gcaggatttt taaggaccgg ttaagtacag gtgtgacccc tgtagccgat gcatttattg
     4621 ataattatat ggcagctgcc aacggggagt atgtaaaagt ttatctttac attcttcgcc
     4681 atcaaaacca ggaagatctg acgttagaac agatcgcaga tgccctaaac catacagaag
     4741 cagatgtaaa aagagccatt aattactggc agaaggccgg ggtcttagaa aatgtacagg
     4801 ataatacctt ggaaggtata tcagcagaac aaaatcgtac tagctggtct atagatatag
     4861 aagtaccccc ggcggcatca aaggaaacag ctgcatccca ggcacctgtt tcagctcctg
     4921 cctattctgc agaacaggta agccgtttaa ataaggatga ggaattttca cagcttttat
     4981 acattgcaca gaagttttta aacaaagcgt ttacgcccag ggattgtcag gtatttgcct
     5041 atctttatga cactctcggt atgaatatcg gcttgttaga gtatctggca gaatactgtg
     5101 cccagaatgg ccatacatcg gtgcgctatc tggaaacagt agccttaaac tggcatgaaa
     5161 aaggaatacg cacagccgaa gaagcccagg aatacagtac tgcgtataca aaagacgctt
     5221 ttgcggtcat gaaagctttt ggtttaaaca gccgcaaacc ggcagtccca gagcagaaga
     5281 tcatggaaaa atggtttaag gattatggct ttgaccgtga actggtctta gaagcatgca
     5341 gtcgtaccat taatgcgatc cataccccaa gttttcagta tgcagactcc attcttacag
     5401 actggaagaa agcgggcgtg aaaaccttag cagatgtaaa gggaatggat gcccgcaggg
     5461 cggaacgtgc ccaaaatggt gctgttaagc ggcttcagag ttatggaaac ggggcagcag
     5521 cacaaaataa cagaaaaact tcccaaaatc agtttcacaa ttttaaacag agggatacag
     5581 attatgacgc cctgatgctt cagaatgtga aagaatggat ggacagtccg gaataataag
     5641 gagacagact tatggcattg agcaattctc agtatgacgc cattatgaga gaatatggaa
     5701 aacagcagat ggaaaaccag cacagtcagg aagcgcggaa aaaagagatc tatcaaaaag
     5761 ttccggctgt aaaggaatta gaggcagaga tcgcggagcg gtccgttgcc tgtgccagaa
     5821 aacttttaga gggaaatcag gaagcaatct acgaacttcg ggaaaagctg gcagatctga
     5881 aggaacagaa ggcccttctt atcaaggcag ctggttattc ggatgattat ttagaactcc
     5941 attaccgctg taaagactgc agggataccg gctgggtgga tggccacaag tgccattgct
     6001 ttttgcgggc ccagatgaaa ctgctttatg cccagtctaa tctggaacgt gtgctggaac
     6061 aggaaaattt tgatcatctt acatttcagt attatgataa cagccagatc attccggaga
     6121 ttggtatgac caatgcagat tatatgcgcc gggtggtaaa aagctgtatg gaatttgcac
     6181 agaattttga cagtcaccag gacaacctgc tttttacagg aagcacagga gtgggaaaaa
     6241 catttctcac caactgcatt gccagaaagc
//
LOCUS       DBMDUA010000160         6158 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5169_length_6158_cov_2.115569, whole genome shotgun sequence.
ACCESSION   DBMDUA010000160 DBMDUA010000000
VERSION     DBMDUA010000160.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5169_length_6158_cov_2.115569"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="ACZPCL_10715"
     CDS             complement(<1..370)
                     /locus_tag="ACZPCL_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162974.1"
                     /translation="MNQLKRYEDLPFQKKLFFVFGFSLVFCIVVAVFVLTDYCTRILV
                     RNNIDNFTVISQQAGIDFNRRVSDTEKQLFNNITMFQIPDSIAACDADKSDYKKRELK
                     YRLNQIVAENTYFDYACLVTD"
     gene            complement(367..1806)
                     /locus_tag="ACZPCL_10720"
     CDS             complement(367..1806)
                     /locus_tag="ACZPCL_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1162975.1"
                     /translation="MAYKAVLIDDNKNTVLSLEYSIEWENLGIELAGTAYDGRSGKEL
                     IEKTDPDIIISDINMPYLDGFTMVEESGKAEDRIIIVITGYDKFQYASRAIKLAVFDY
                     ILKPIDDEELTDSLKRAVRKLDKKHSLENQKINTVKAMMTVAITNHRDDTVGNITESE
                     KEPVKRGLVMIGALKGEPSKALLERVETLTLLSSGNAYSIVTEERIILLVTEKRENSG
                     WQAEIEKLKEKLTTLEELSALGISSEKEETASFYSLYLEAYEDLLKKSKKENHMQVSD
                     LQENALRLADSIKGMEDYERIIEEFLMCTSGSYVAIQIMALFFCGRIMEEHKTWRGEL
                     DHLSFEVAGITSQKMFASWLKTFLAKIDEIREKSSGKSELVLLAVRYIKNHCLESLRL
                     ETVAEELAVSPNYLSTIIKKETGITFQQHMIQEKLNIARKLLDDTRMTIDEISIAVGY
                     ENYISFYNAFRKNVHMTPSEYRMKKGNCQ"
     gene            complement(1829..2668)
                     /locus_tag="ACZPCL_10725"
     CDS             complement(1829..2668)
                     /locus_tag="ACZPCL_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1162976.1"
                     /translation="MGTTKKRHLLSVIQKVLITCVMLVVISPFYISIVYALKNKNDLT
                     INRLAWPTHPTLMNFQRVITENDQFLTGYKNSILTTVPVVVLLMLITSMAAYVLARYT
                     TRVYKWIYLIFISGMLIPIQCIMLPFYINFFKLNLTNSNLGFVIARTGLQIPISILVV
                     TGFVKAIPLDLEESARIDGCNRFKMFWKVVFPLMKPVNATQLVLNTLFVWNDYTTAII
                     LLRKPFARTLILAQMAYFNEYTSDLNLAFAFFILATLPILIVYFSMQKYVVGGIMMGA
                     VKG"
     gene            complement(2668..3534)
                     /locus_tag="ACZPCL_10730"
     CDS             complement(2668..3534)
                     /locus_tag="ACZPCL_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1162977.1"
                     /translation="MKQKRLVKKLRTHAGNFLFLFPAMLLYCFVVIVPFIQGIPYSFT
                     NRASVVSQSYDFVGLRNYMELITNKYFQQAFFHTVQFTVIYVIGANVLGLALALMLSR
                     SSRFNNLARTIMFIPFTVALTSGAIVWSYVFTDVYSPLFQKMSPLGLSSQVVPAMAII
                     AIWRDMGYCMLIYLAGLQSISGEYYEAAKVDGATWWQRTRLITFPLILPAIVSNVTLL
                     IAWGLKCFDYPMAVARNMEAAQTVSMYVYDYIFGFNKAGIGQAAAIIITVVLVVITQC
                     ITSIGAKLEVDQ"
     gene            complement(3575..4585)
                     /locus_tag="ACZPCL_10735"
     CDS             complement(3575..4585)
                     /locus_tag="ACZPCL_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1162978.1"
                     /translation="MYYVDEYKADRKRYETMQYNRCGKSGLKLPAVSLGLWHNFGSKD
                     NYDMMKNLCFTAFDNGITHFDLANNYGPIYGSAEENFGRIMRDFLRPYRNELIISTKA
                     GYDMWDGPYGNWGSRKYLIASLDESLKRMQLDYVDIFYHHRMDPETPLEETILALDQI
                     VRSGKALYAGISNYDGEHAKKALEIAKEIHLPLIINQNRYNIFDRTVEKNGLKDYARE
                     SGMGLICFSPLAQGMLSEKYINGIPADSRVARDGRFLHASDITPEKIEKIKKLDALAK
                     ERGQSLPQMALSWLLKDSDVTSVLIGASKPEQILSNIGIVGHTTFTKEELDLIDQISK
                     EQ"
     gene            complement(4625..5578)
                     /locus_tag="ACZPCL_10740"
     CDS             complement(4625..5578)
                     /locus_tag="ACZPCL_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR1162979.1"
                     /translation="MDKIERVTRAFKGEEVDHIPVCLWKHIPPTLWKDELFIKAQMDF
                     YKETDVDFIKLSADKYFCWPAPVLKDIQSAKQLYDIKPLGAHHPHIRGQINRTKKIME
                     EINGECLAFYLIFCPLSYLRLEIGYPKMMQFMEEDPEAVLYAENVIAQDVKVLVKGII
                     EEAKVAGIFYSVQNAEENRFTYETYRKYITPPEKEVLDYANTLSDMNVLHCCGWEGIP
                     NRLEDWADYKTAVVSWARYIEGLDVKEAKEKFGRTVWGGFDNRPGSLIYTGTKEEIEA
                     EVKRLVEASGKRGYIIGPDCSIHDELPEERIKWIVDAARKL"
     gene            complement(5685..>6158)
                     /locus_tag="ACZPCL_10745"
     CDS             complement(5685..>6158)
                     /locus_tag="ACZPCL_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162980.1"
                     /translation="WYKNLGEMLAKSEGTDFEFDFFLPPIDDEGSAKLCTMVDQSFMV
                     NPNSDCAEEAVEFLEYWVTDGALTWSETTMMPLITGESSDKLLPVVKSMAELKANSSI
                     SQGIFTKPFNTEFVNAWRKGLISYAESVCTGKPMTHEECLNNIQSLFDDIIATSK"
BASE COUNT         1635 a         1459 c         1126 g         1938 t
ORIGIN      
        1 catctgtaac cagacaggca taatcaaaat atgtattttc cgcaacgatc tggtttaaac
       61 ggtatttcag ttctctcttt ttataatcac ttttatctgc atcacaggct gctatactgt
      121 ccgggatctg aaacatggta atattattaa acagctgttt ttctgtatca gacacccgcc
      181 ggttaaaatc gatcccggcc tgctggctga tcacggtaaa attatcaata ttattgcgca
      241 ccaggatcct ggtacagtaa tccgtaagca caaacacagc tactacaata caaaatacca
      301 aactgaaccc aaatacaaaa aacagttttt tctgaaacgg caggtcttca tatcttttta
      361 actggttcat tgacaatttc cttttttcat tctgtattca ctgggtgtca tatgcacatt
      421 tttgcggaaa gcattataaa aactgatata gttttcatat cccactgcta tggatatttc
      481 atcaatggtc attctggtat catctaaaag ttttcttgct atatttaact tttcctggat
      541 catatgctgc tggaatgtga tccccgtttc tttctttatg atcgtagaaa gataattggg
      601 gctgactgcc agttcttccg ccacagtttc cagcctcagg ctttccagac agtgattttt
      661 tatatatctt actgccagca gtaccagttc tgactttccg ctgctttttt cccggatctc
      721 gtcaatcttt gccagaaacg tctttaacca gctggcaaac attttctgtg aagtaatacc
      781 tgccacctca aaggacagat gatccaattc ccctctccag gtcttgtgtt cctccataat
      841 acggccgcaa aagaaaagag ccatgatctg tatggcaaca tagcttcctg aggtacacat
      901 aaggaattct tcgatgatcc gttcataatc ttccattcct tttatactgt ctgcaaggcg
      961 gagggcattt tcctgcagat cagagacctg catatgattt tcttttttac ttttcttcag
     1021 aagatcctca tatgcctcta aatataacga atagaaggaa gctgtctcct ctttctcact
     1081 gctgatacca agggcagaca gttcctccaa cgtggttaac ttctctttta atttttctat
     1141 ttctgcctgc cagccactgt tttccctttt ttccgtgact aacaggatga tccgttcttc
     1201 tgttacaatg gaataagcat ttccggaaga taataaagtc agtgtttcaa cacgctccaa
     1261 aagggccttg gatggctctc ctttcagagc gccgatcatc accaagcccc tttttactgg
     1321 ttccttttca ctttctgtga tattgcccac cgtatcatcc cggtggtttg tgatggctac
     1381 tgtcatcatt gcctttactg tattgatctt ctggttttcc aggctgtgct ttttatccag
     1441 cttacgcaca gcccgcttca aactgtcagt taattcctca tcatcaatgg gttttaaaat
     1501 ataatcaaat acagccagtt taatggcgcg ggatgcatac tggaatttat catatcccgt
     1561 gatcacgatt atgatcctgt cttctgcctt accggactcc tccaccatgg taaatccatc
     1621 cagatatggc atattaatat cactgatgat aatatccgga tccgttttct cgatcagctc
     1681 tttaccgctt cttccatcat aagcagttcc cgcaagttcg atccccaaat tttcccattc
     1741 aatggaatat tcaagggaaa gaaccgtatt tttattatcg tcgatcaaaa ctgctttata
     1801 ggccatattt tctcccttta tcctgtcatc acccttttac tgctcccatc attataccac
     1861 ctaccacata cttctgcata ctgaaatata cgatcaggat cggtaatgtc gccaggataa
     1921 aaaaggcaaa tgcaaggttc agatcggaag tatattcatt aaagtaagcc atctgggcaa
     1981 ggatcagtgt tctggcaaac ggctttctta aaaggatgat ggctgttgta taatcattcc
     2041 atacaaacag tgtgtttaat accagctggg ttgcatttac cggcttcatt aacgggaaaa
     2101 caactttcca gaacatttta aaacggttac atccatcgat ccttgctgac tcttccagat
     2161 ctaaggggat cgccttcaca aaaccggtca ctaccaggat gctgataggg atctgcagtc
     2221 cggtccttgc gatcacaaag cctaaattgg agtttgtaag gttcagtttg aaaaagttaa
     2281 tataaaatgg aagcatgata cactggatcg ggatcagcat accggatata aaaataagat
     2341 agatccattt atataccctg gtcgtatatc gtgccagcac ataagctgcc atggatgtga
     2401 tcagcatcag aaggaccact accggcaccg tagtcaggat actgttttta tatcctgtaa
     2461 ggaactggtc attttccgtg atcacacgct ggaaattcat caaggtagga tgtgtcggcc
     2521 atgccaggcg gttgatcgta aggtcatttt tatttttcag cgcatatacg atactgatat
     2581 aaaaaggtga gatcacgacc agcatgacac atgtgatcag aactttctgt attacagata
     2641 ataaatgtct ttttttcgta gttcccatta ttgatccacc tccagtttag ctcctatgga
     2701 tgtaatgcac tgggtaatga ccaccagcac tacggttatg atgatcgctg ctgcctgacc
     2761 aatgcctgcc ttgttaaagc caaatatgta atcatataca tacatggata cagtctgtgc
     2821 tgcctccata tttcttgcta ctgccattgg ataatcaaaa catttaaggc cccaggcgat
     2881 cagcagtgtc acattagaaa cgatcgccgg aaggatcagg gggaaggtga tcagtcttgt
     2941 tctctgccac caggtagcgc cgtctacttt tgctgcctca taatattctc cggaaataga
     3001 ctgaagccca gccagataga tcagcatgca gtaacccata tctctccaga tggcgataat
     3061 ggccatagcc ggaactacct gggaagaaag tcccaaagga ctcattttct gaaacagagg
     3121 agaatataca tctgtaaata cataactcca tacgatagca ccactggtaa gggctactgt
     3181 aaatggaata aacataatgg tccttgccag gttattaaac ctggaagatc ttgatagcat
     3241 cagtgctaat gccagtccca gcacattggc cccgatcaca tagataacgg taaactggac
     3301 ggtatgaaaa aatgcctgct ggaaatactt gtttgtgatc agttccatgt agtttctcag
     3361 acctacaaaa tcataactct ggctcactac actggcacgg tttgtaaatg aataaggaat
     3421 accctgtata aatggaacga tcactacaaa acagtacaac aacatggcgg gaaaaagaaa
     3481 taagaaattc cctgcatgag ttcgcaactt cttcaccaat cttttctgtt tcattatctt
     3541 ttccccgcct ctctttttat tcctgcttga tcacttactg ctctttggag atctggtcga
     3601 tcagatccag ttcttctttt gtaaatgttg tatgtcccac aattccaata ttacttaata
     3661 tctgctccgg cttagacgca ccgataagta cactggtaac atcactgtct tttaacagcc
     3721 aggaaagtgc catctgtgga aggctctgtc ctctttcttt tgcaagagca tccagcttct
     3781 taatcttttc gatcttttcc ggagtaatgt ctgatgcatg gagaaatctt ccgtctcttg
     3841 ccacacggct gtcagctggg ataccgttaa tgtatttttc tgataacatt ccctgtgcca
     3901 gaggactgaa gcagatcaga cccattcctg actcacgggc ataatctttt aagccatttt
     3961 tctccactgt acgatcaaaa atgttgtaac ggttctgatt gatgatcagc ggcaggtgga
     4021 tctcttttgc aatctctaat gccttctttg catgttcccc gtcataatta gaaattcctg
     4081 catacagtgc ttttccggag cggacgatct gatctaaagc caggatcgtt tcctctaatg
     4141 gtgtctccgg gtccatcctg tgatggtaga aaatatccac atagtctaac tgcattctct
     4201 ttaaggactc atccaggctg gcaatgagat attttctgga accccagttt ccgtaagggc
     4261 catcccacat gtcatatccg gcttttgtac tgatgatcag ttcatttctg tatggacgta
     4321 aaaaatcacg catgatccgt ccaaagtttt cctctgcaga tccgtagatc gggccatagt
     4381 tatttgccag gtcaaaatga gtaataccat tgtcaaaagc tgtaaagcaa aggttcttca
     4441 tcatgtcata gttatctttg gaaccgaaat tgtgccacag tcctaaagat actgccggaa
     4501 gtttcagtcc gcttttgccg cagcgattgt actgcatggt ctcatatctt tttctgtctg
     4561 ccttatattc atcaacgtaa tacataaaat tctccttttt atatctatac tttttaagaa
     4621 cttatcagag ctttcttgct gcgtctacga tccatttaat gcgttcttcc ggaagttcat
     4681 catggatgct gcagtccggt ccgataatat agcctctctt gccgcttgcc tcaaccagac
     4741 gttttacttc tgcctcgatt tcttcttttg ttcctgtgta gatcagactt cccggccggt
     4801 tgtcaaagcc gccccataca gtccgtccga atttttcttt tgcttctttt acatccagac
     4861 cttcaatata tcttgcccag cttactactg ctgtcttata gtccgcccag tcctctaagc
     4921 ggtttggaat accttcccat ccacagcagt ggagcacgtt catatcactt aatgtattgg
     4981 cataatctaa aacttctttc tccggcggag tgatgtattt tctgtatgtc tcatatgtaa
     5041 agcggttttc ttctgcattc tgtacgctgt agaagatacc tgcaaccttt gcctcttcta
     5101 tgatcccttt tacaaggacc tttacatcct gggcgatcac attttccgca tataatacag
     5161 cttccggatc ttcttccata aactgcatca ttttcggata tccgatctcc agacgtaaat
     5221 aagataacgg gcagaagatc agataaaatg ccaggcactc accattgatc tcttccatga
     5281 tcttctttgt acggttgatc tggcctctga tgtgtggatg atgggctccc aacggcttta
     5341 tatcataaag ctgttttgca gactggatat cttttaacac aggtgccggc cagcagaaat
     5401 atttatctgc ggaaagtttg ataaaatcaa catctgtttc cttgtaaaaa tccatctgcg
     5461 ccttgataaa caactcatct ttccataaag taggcggaat gtgtttccac aaacacaccg
     5521 gaatgtgatc cacctcttcc cctttaaatg cccttgtcac tctctctatt ttatccatgt
     5581 aagatttcct ttcttctgaa cagaggccgc tgcaatacga tctcctaaga tcctgcagca
     5641 gcccctgttt agcatctaca cttattattt tctgcttatt ttacttattt gcttgttgcg
     5701 ataatatcat caaacaggga ctgaatattg ttcagacact cttcgtgagt catcggcttg
     5761 cctgtgcata cgctctctgc ataagagata agtccttttc tccatgcatt tacaaattct
     5821 gtgttaaatg gttttgtaaa gattccctgg gaaatagagc tgtttgcctt caattctgcc
     5881 atggatttaa ccaccggaag aagcttatca gaagactcac cggtgatcag aggcatcatg
     5941 gttgtttcag accacgttaa agcaccatct gttacccaat actctaaaaa ttcaacagcc
     6001 tcttctgcac aatcagaatt tggattcacc ataaaggact ggtctaccat tgtacacagc
     6061 tttgcgcttc cttcatcgtc gatcggaggc aggaagaaat caaattcaaa atcagttcct
     6121 tcggatttag caagcatttc acctaaattc ttatacca
//
LOCUS       DBMDUA010000161         6138 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5189_length_6138_cov_2.157694, whole genome shotgun sequence.
ACCESSION   DBMDUA010000161 DBMDUA010000000
VERSION     DBMDUA010000161.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6138)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5189_length_6138_cov_2.157694"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            80..532
                     /gene="nrdR"
                     /locus_tag="ACZPCL_10750"
     CDS             80..532
                     /gene="nrdR"
                     /locus_tag="ACZPCL_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR1162981.1"
                     /translation="MKCPYCNEADTKVIDSRPADDNSSIRRRRQCERCGKRFTTYEKL
                     ETMPLMVIKKDDSREAYDRSKIEAGILHSCHKRPVSPRQISTMIDEIENQIFNREEKE
                     IPTSVIGELVMDKLKSVDEVAYVRFASVYREFKDVSTFMEELGKLLKK"
     gene            557..1057
                     /locus_tag="ACZPCL_10755"
     CDS             557..1057
                     /locus_tag="ACZPCL_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HIR1162982.1"
                     /translation="MFQCFYPSKMEESSYVIPYENWKKMGIQGIVYDIDNTLVPHGAP
                     ADERAIRLFEHLRGLGLKTCLLSNNKEPRVKSFAQQVKCPYIYKGGKPGAKGYKKAME
                     TMGTDVKNTLFVGDQLFTDIWGANRLGIQTVLVRPINPKEEIQIVLKRYLEKPVLYCY
                     RKSLEK"
     gene            1116..2504
                     /gene="aroE"
                     /locus_tag="ACZPCL_10760"
     CDS             1116..2504
                     /gene="aroE"
                     /locus_tag="ACZPCL_10760"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR1162983.1"
                     /translation="MDGKTKVYGVMGDPIEHSMSPLMHNFYAERTGTDLVYVPFHVNR
                     GTVEMAVKGAFALNIQGMNVTVPHKQDVMKCLEAIDEDAKAIGAVNTLVRTEHGYKGY
                     NTDGAGLKRAMDEAGISIAGEKCILIGAGGAAKAAAYILAKSGASVVYILNRSVEKAA
                     ALADYINGLMGRAVLIPLKLEEYDQIPQEEKGYLAIQSTSVGMHPHTEDVIIEDEAFY
                     KLIHTAVDIVYTPSCTKFMKMVQAAGGKAINGLDMLLYQGLIAFELWNPEVKVDKDTI
                     DRIREMILDHLHGNTRKHNVIFTGFMGAGKTSAGHFYADHYHMPFIDTDQEIEKEAGM
                     AISQIFKEQGEEAFRKLETQCLEKLLRTTDGAVISVGGGLPLREENRELLKKLGLVVY
                     LDVSPETVYKRIGADVSSRPMLHSDDVPGRIRSLLNERRPLYLKAAHVAVDVNDRSLD
                     AITEEIYREAHK"
     gene            2656..3213
                     /gene="efp"
                     /locus_tag="ACZPCL_10765"
     CDS             2656..3213
                     /gene="efp"
                     /locus_tag="ACZPCL_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR1162984.1"
                     /translation="MISAGDFRNGITLEIDGQVVQILEFQHVKPGKGAAFVRTKLKNV
                     INGGVVERTFRPTEKFPAARIDRVDMQYLYEDGDLYNFMNVENYEQLALNKDIIGDAL
                     KFVKENEICKVCSYNGNVFSVEPPLFVELEITDTEPGFKGDTATGASKPATVETGATV
                     SVPLFVNTGDKIKIDTRTGEYLSRV"
     gene            3334..5121
                     /gene="argS"
                     /locus_tag="ACZPCL_10770"
     CDS             3334..5121
                     /gene="argS"
                     /locus_tag="ACZPCL_10770"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR1162985.1"
                     /translation="MKKILEALEQELKAAFTACGYEEKFAKVVLSNRPDLCEYQCNGA
                     MAAAKAYKKKPIDIANAVVEQLTGEHASSVFSEVVAVMPGFINLKLSEGFLADYMNGM
                     AADEKLGLGEPEKKETIIVDFGGANVAKPLHVGHLRSAVIGESVKRMGRFLGHNVIGD
                     VHLGDWGLQMGLIIEELRDRKPELPYFDENYTGEYPAEAPFTISELEEIYPAASGKSK
                     VDEAFKERAHQATLKLQKGYPPFRAIWKHIMNVSVADLKKNYSNLNVDFDLWKGESDA
                     EPYIQKMIDTLEAQGLVHESQGALVVDVAEESDTKEIPPCLIRKSDGASLYATSDLAT
                     IVEREEDFKPDRYIYVVDKRQAMHFEQVFRVAKKAGIVKPETKMVFLGFGTMNGKDGK
                     PFKTREGGVMRLEKLIADIDEAVYQRIMENRTVSEEEAKETAKIVGLAALKYGDLSNQ
                     ASKDYIFDTDRFISFEGNTGPYILYTIVRIKSILNKYKENGGQVTEEGSEKKILPAKG
                     ASEKALMLQLSKYNEVIENGFAETAPHKICQYIYELANAFNSFYHDTKILSEEDQAVK
                     DSYIGLITLTRRTLEICIGLLGIEAPERM"
     gene            5143..>6138
                     /locus_tag="ACZPCL_10775"
     CDS             5143..>6138
                     /locus_tag="ACZPCL_10775"
                     /inference="COORDINATES: protein motif:HMM:NF020044.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HIR1162986.1"
                     /translation="MKIVKMDTNSFWKGEAGVVGLVEDQAHTFKTKLYLKNGQVKDYS
                     CTCEKGNSYRGICAHGEALFAYYKEYQAEMSKPIVHSSSQVHTMIREYTNQEVARILE
                     EEEGSQVKLVPAVILNGRDVRLEFKVGREKMYAVRDLAAFSDAVAVGAYVEYGKELAF
                     HHQGSSFCPECRGLLSLVMALTEGQKSQRDISLSRMNRERFFEVLQQEELEVQLPGGI
                     RSQLKVQKKDPKLVIRIRRYGRDGVEAVLEGVRTDEEGDTEPVLAFFRGERRIYIVTG
                     KTLCCCSHHFSQAAGTFLEQITKEREYRIQAGAKDIPLLYERVLKTLKPYSIMIQE"
BASE COUNT         1916 a         1195 c         1584 g         1443 t
ORIGIN      
        1 ttgcgcttga gggggctttc ggttaaaata aaatcatcat tttgtaaaaa atacatgaga
       61 gaaaggatgt gcagagttta tgaagtgccc gtactgtaat gaagcagata ccaaagtgat
      121 cgattcccgt ccggcagatg ataacagttc cattcgcagg cgccgtcagt gtgaacgctg
      181 tggcaagcgt tttactacgt atgaaaaatt agagaccatg ccccttatgg tcattaaaaa
      241 agatgattcc agagaggcat atgaccgctc caaaatagaa gcaggcattc tgcattcctg
      301 tcataaacgt ccggtatctc cccgtcagat cagtaccatg atcgatgaga tcgaaaacca
      361 gatctttaac agggaagaaa aggagatccc aacttctgtg atcggtgaac tggtcatgga
      421 caaactgaaa agtgtagacg aagtagctta tgtgcgcttt gcctctgttt acagggagtt
      481 taaggatgta agtaccttta tggaagaatt gggaaaactt ttaaagaaat aagtctaaaa
      541 gaaagtagaa tttaatatgt ttcaatgttt ttaccccagt aaaatggaag agtcctctta
      601 tgttattcct tatgaaaact ggaaaaaaat ggggatccag gggatcgttt acgatattga
      661 taatacttta gtcccgcatg gggcaccggc ggatgagaga gccatccggc tttttgagca
      721 tctgagaggc ttgggattga aaacctgcct tctttccaat aataaagaac cgagagtgaa
      781 atcttttgcg cagcaggtaa aatgccctta tatttataaa gggggaaaac caggggcaaa
      841 aggctataaa aaggccatgg agaccatggg gaccgatgtg aaaaatacgc tgtttgtagg
      901 agatcagctt tttacagata tctggggcgc caaccgtctg gggatccaga ctgtcctggt
      961 acggcccatc aacccaaaag aagagatcca gatcgtatta aaacgttatc tggaaaaacc
     1021 ggttttatac tgttacagga aaagccttga aaagtaagat ccagacaaaa gaaaatcgtg
     1081 tgaaaaagta aaaagagaca gcaggagata acgcaatgga tggaaaaaca aaagtatacg
     1141 gtgtaatggg agatcccatc gaacattcca tgtcaccgct tatgcataat ttttacgcag
     1201 agcgtacagg aacagactta gtctacgttc cctttcacgt aaacagggga actgtggaaa
     1261 tggctgtaaa aggtgccttt gctttaaata tccagggaat gaatgtaacc gttccacata
     1321 aacaggatgt aatgaagtgc ttggaagcca ttgatgaaga cgcaaaagcc atcggtgctg
     1381 taaataccct ggtgcgtaca gagcatggat acaaaggtta taatacagac ggtgcaggct
     1441 taaaacgtgc tatggatgaa gcaggtattt ctattgcagg agaaaaatgt atcctcatag
     1501 gagccggagg cgcagccaag gcagcagcct acatccttgc aaagtccggc gcgtccgttg
     1561 tatatatctt aaaccgaagc gtggagaaag cagcggcttt agctgattat attaacggac
     1621 tgatgggccg tgcagtgctt atcccgttaa aactggaaga gtatgaccag atccctcagg
     1681 aagaaaaggg ctatctggcg atccagtcaa ccagcgtagg catgcaccct catacagagg
     1741 acgttattat agaagatgaa gctttttata agctgatcca cacagcagtg gacattgtat
     1801 acacaccttc ctgtacgaag tttatgaaaa tggtacaggc tgcaggggga aaagcaatta
     1861 acggtctgga tatgcttttg tatcaggggc tgatcgcgtt tgaactgtgg aatccggagg
     1921 taaaggtaga taaagatacc attgacagga tccgtgaaat gatcctggat catttacatg
     1981 gaaataccag aaagcacaat gtgatcttta ctggctttat gggagcagga aagaccagcg
     2041 caggccattt ttatgcagat cattatcaca tgccgttcat tgatacagat caggagatcg
     2101 aaaaagaagc aggtatggcc atttcccaga tctttaagga acagggagag gaagccttca
     2161 gaaagttaga aacacagtgc ctggaaaagc tgttaaggac cacagacggg gcagtgatct
     2221 ctgtgggcgg cggacttcct ttaagagagg aaaaccggga acttttaaag aagctgggac
     2281 tggtagtata tctggatgtt tcaccggaga ctgtttataa gcggatcggt gcagatgtgt
     2341 ccagccgtcc tatgctgcac agtgatgatg tgcccggacg gatcagaagt ttacttaatg
     2401 aacgcaggcc attatactta aaagcagccc atgtggcagt ggatgtaaat gaccggtctt
     2461 tagatgccat cacagaggaa atctacaggg aagcccataa ataagtggct ggtggctgta
     2521 aaatcatcca gaataagctg taaaacagaa aaaagaactt gaaaaacgta catacatagt
     2581 ataaaataaa aaagattaga gagaaattag aggatgcagg cagcatcctg aacgaactta
     2641 aggaggaata tctttatgat atcagcaggc gattttagaa acggaattac actggaaatt
     2701 gatggacagg ttgttcagat ccttgaattc cagcacgtta agccaggcaa gggcgctgct
     2761 ttcgtgcgta ccaaattaaa aaatgttatc aacggcggtg ttgttgagcg tactttccgt
     2821 ccaaccgaga agttcccggc agcaagaatc gaccgtgtag atatgcagta tctgtatgag
     2881 gatggagatc tgtataactt catgaacgta gagaactacg agcagttagc attaaacaag
     2941 gacatcattg gcgatgcatt aaagttcgtt aaagagaacg agatctgcaa ggtttgttct
     3001 tacaacggca acgtattctc tgtagagcca cctctgtttg tagagctgga gatcactgat
     3061 acagaaccag gcttcaaggg tgatacagca actggcgcaa gcaagcctgc aactgtagag
     3121 accggagcta ctgtaagtgt tccgttattc gtaaacacag gtgataagat caagatcgat
     3181 accagaaccg gcgaatatct gtcccgtgta taagcagatt attgcttaga taaaaagtaa
     3241 aagattgctc cggcagaccg ccggagcgat tttcgtatat gagatatact cccggggagt
     3301 acatcaagaa aagaatacaa ggagaatgat attgtgaaaa agattctgga agctctggag
     3361 caggagctga aagcagcatt tacagcctgc ggctatgaag aaaaattcgc aaaagtggtt
     3421 ttatccaacc gcccggacct gtgcgaatat cagtgcaacg gcgctatggc agctgccaag
     3481 gcatataaaa agaaacctat cgacattgca aatgcagtgg tagaacagct tacaggagaa
     3541 catgcaagtt ctgtattttc tgaggttgtg gcagtaatgc ctggttttat caacttaaaa
     3601 ctttctgaag gctttttagc tgactatatg aacggtatgg cagcagatga gaagctgggt
     3661 ctgggagaac cggaaaagaa agagaccatt atcgtagact ttggcggtgc caatgtggca
     3721 aaacctcttc atgtaggaca tttgcgttct gcagttatcg gtgagagcgt aaagcgcatg
     3781 ggacgtttcc ttggccacaa tgtgatcggc gatgtacatt taggcgactg gggactgcag
     3841 atgggcctta ttatcgagga actgcgtgac cgcaagccgg aactgccata ctttgatgaa
     3901 aattatacag gagaatatcc agcagaagct ccttttacca tcagcgagtt agaggagatc
     3961 tacccggctg ccagcggcaa gtccaaggta gatgaggcat ttaaggagcg cgcccatcag
     4021 gcaaccttaa agcttcaaaa gggatatccg ccattcagag ccatctggaa gcatattatg
     4081 aatgtttctg tagcggacct taagaagaac tactccaacc tgaacgtaga tttcgacctg
     4141 tggaagggcg aaagcgatgc agagccatat atccagaaga tgatcgacac tctggaagca
     4201 cagggactgg tacatgaaag ccagggcgct ttagttgtag atgtagcaga agaaagtgat
     4261 accaaggaga ttccgccatg cctgatccgt aagtcagacg gtgcttccct gtatgcaact
     4321 tctgaccttg ctaccatcgt agagcgtgag gaagacttta agccggaccg ctacatttac
     4381 gtagtagata agcgtcaggc gatgcacttt gagcaggtgt tccgtgttgc aaagaaggcc
     4441 ggaatcgtga aacctgagac taagatggta ttcttaggtt tcggtactat gaacggcaaa
     4501 gacggaaagc catttaagac aagagaaggc ggcgttatgc gtctggagaa gctgatcgca
     4561 gatattgatg aggctgttta ccagagaatt atggagaacc gcactgtatc tgaggaagaa
     4621 gcaaaagaga cagcaaagat cgtaggtctt gcagctttaa aatacggaga tctgtccaac
     4681 caggcatcca aggattatat ctttgataca gaccgcttta tttcttttga gggaaataca
     4741 ggtccataca tcctttatac catcgtccgc atcaagtcca ttttaaataa atataaggaa
     4801 aatggcggac aggtaacaga agagggctct gaaaagaaga tccttcctgc aaagggcgct
     4861 tctgagaagg cactgatgct tcagctttcc aagtacaatg aagtcatcga aaacggcttt
     4921 gcagagacag ctcctcataa gatctgtcag tatatttatg agctggcaaa tgcatttaac
     4981 agcttttacc acgatacaaa gatcctctct gaggaagacc aggcagtaaa agacagctac
     5041 atcggtctga tcacactgac cagaagaaca ctggagatct gcatcggact tctgggaatc
     5101 gaggctccgg agcggatgta aagaagggat agatcaacgt caatgaagat cgtcaaaatg
     5161 gatacaaaca gtttctggaa aggcgaagct ggcgtagtgg gtctggtgga ggaccaggcc
     5221 cacaccttta aaacaaagtt atatctgaaa aatggtcagg taaaggatta ttcctgtacc
     5281 tgtgaaaaag ggaattctta caggggcatc tgcgcccatg gggaggccct ttttgcatat
     5341 tataaagaat atcaggcgga aatgtccaag cctatagtac acagttcttc ccaggtccac
     5401 acgatgatcc gggagtatac caaccaggag gtagcccgca tcttagagga agaagaggga
     5461 agccaggtaa aactggtgcc tgctgtgatc ttaaacggaa gggatgtccg gctggaattt
     5521 aaagtaggcc gtgaaaaaat gtatgcagta agggatctgg cagctttttc cgatgcagtg
     5581 gctgtaggcg cctatgtgga atacggaaaa gaattggctt tccatcacca gggaagttcc
     5641 ttctgtccgg aatgcagggg acttttgtct ttagtcatgg cactgacaga gggacagaaa
     5701 tcccagaggg atatatcctt aagccgtatg aaccgggaac gtttttttga ggttttacag
     5761 caggaagaac tggaggtgca gcttccggga gggatccgtt cccagcttaa agtacagaaa
     5821 aaagatccga aactggtgat ccgcatccgc agatatggaa gggacggcgt ggaagctgtg
     5881 ttagaaggtg tccggacaga cgaagaaggg gacacagagc cggtactggc ttttttcagg
     5941 ggagagcggc gcatttatat tgtcacagga aagaccctct gctgctgttc ccaccacttc
     6001 agccaggcgg caggtacctt tttggagcag atcacaaagg agagggaata ccggatccag
     6061 gcaggagcaa aggacattcc tcttttatat gagcgtgtac ttaagacatt aaagccctac
     6121 agcatcatga tccaggaa
//
LOCUS       DBMDUA010000162         6064 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5255_length_6064_cov_1.811169, whole genome shotgun sequence.
ACCESSION   DBMDUA010000162 DBMDUA010000000
VERSION     DBMDUA010000162.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5255_length_6064_cov_1.811169"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="ACZPCL_10780"
     CDS             complement(<1..869)
                     /locus_tag="ACZPCL_10780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR1162987.1"
                     /translation="MLKSMTGFGRCENATDEYKISVEMKAVNHRYLDLSIKMPKKFNY
                     FEAAIRTLLKKYIQRGKVDLFINYEDYTEGNLCLKYNKALAGEYMDYFKKMEEEFGIR
                     NDIQVSTLSRCPEVLTMEQVPDDEEHLWEILSAALEEAAKKFVASREVEGAHLKEDLL
                     GKLDYMTGLVDFIEERSPVILKEYRQKLEDKVKELLNGTAIDEGRIAAEVVIYADKIC
                     VDEETVRLRSHIENTRQELLSGESVGRKLDFIAQEMNREANTILSKSSDLAISDKAIA
                     SQTVGGQGGGGRP"
     gene            complement(955..3267)
                     /locus_tag="ACZPCL_10785"
     CDS             complement(955..3267)
                     /locus_tag="ACZPCL_10785"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase TgpA family protein"
                     /protein_id="HIR1162988.1"
                     /translation="MAGVIQEISSGKNNKNRDILNALPLCMILVAGSILSWFKIAAID
                     LVFEAAAFGIAEIVLCLTAVYGLLSSKDKKWAFFIPAAGAAVLTFIRPVQILNGFLSF
                     LNICIYSWNEKYEDGVRLFGNVQATEQNLFLYSLVFLLILAALLWYLVSVKACTRISL
                     IALVLFVPEIVLGRSSLFGAVLMLTSITGVWLFAFHAGSFGRRTGWTIVTGLILFCML
                     WISGGKTSQTVLKVKKDAAQMTEQLRYGEDNMPSGSLAQAASLEQGDEPRLRVKTEQI
                     KPLYLKGFTGSVYENDSWKPLAKAAYGGNRWGFLQWLNGRGFQPEHQYIAYEEAGRSG
                     TDPLPEDAPWVNHIQVVNTGAMRKYIYEPYSSQAVVNSTNERDEGSRSLAFFGAKRYE
                     ISELSSDMPGELQRLDTWTEAPVTDEQKQYLESEAVYRDFVYVNYLTADPQLSGLIKE
                     LFHKEEEEASLSVYAAVQQIRTVLEENTYYNKYLSEEDTAGDDLLKEFLQGEREGNSA
                     YYTSAAVLALRSFHIPARYAEGYLLTSKQAENSHGDWIGLSSSDGHAWAEVYMDGMGW
                     VPVDVTPGFYYDTYALLNMAQSPGQVRKVAALDSEGEEAENLKKHFPGSEPFLENQKQ
                     TELKTADIGWGMILLILFVLEFIFAVLELRRMYYEYRIRKISDNGSLPSVEFLLLMIY
                     KHLLILGIDMQPGFKSEQIQEKIRETLPEIPDGLYLRVNDLMEKYFYGGEPLEEYEIR
                     LVHQFLLKLRDSRKKMGIFDRLRFRYCIFS"
     gene            complement(3290..4444)
                     /locus_tag="ACZPCL_10790"
     CDS             complement(3290..4444)
                     /locus_tag="ACZPCL_10790"
                     /inference="COORDINATES: protein motif:HMM:NF013998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR1162989.1"
                     /translation="MTRIRIGFGTAVVATAVLFFLSGSYFTAGVFIIEILLPVVLRFF
                     LRLETNGMELEMHTRSACRLGQTIEIDISVKSRWRCLAAARIQADLSYDNKMFQVNRN
                     IPLALDINKRSFGISIPWEPKMCGGAILSLSNVRCYDIFGLNCIKTEFHQVQHLTVYP
                     EKISMRPVASGNQKGRQNEGQQTLSKKGYDATEVFELREYQPGDSIRTIHWKLSEKFD
                     TVLVREPSDTSSYDILVMFDAGLTSGEVTYEPDVLSGAVSEKLMEHKVPHYLGMWAGN
                     KIYLNQITGQQEYDNTMDTWMNLSLQKERGMGLKSLIAEKSHSNYRRILYFTAGTCPD
                     EVYELAKDVSVTAVCILDEEGEIRTTERGMCVLTEIPLEKLTTDTQNIIV"
     gene            complement(4441..5391)
                     /locus_tag="ACZPCL_10795"
     CDS             complement(4441..5391)
                     /locus_tag="ACZPCL_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1162990.1"
                     /translation="MILEQSEHVIQEVKKAFMGKDDIIRRVLATIYAGGHILLEDSPG
                     VGKTTLALAFSRVLGLDYKRIQFTTDTLPSDITGFTMYNRQNEQFEYHEGAAMCNLLL
                     ADEINRTSPKTQSALLEAMEERRVTVDGVTRPLPTPFICIATQNPLGTTGTQALPSSQ
                     LDRFMVRLSIGYPSAEDQIKILKARRYSNPLEEIQVAAQAENILEIQNYLSALKISDD
                     ILYYLIRLCEATREHPLVELVISPRGVWALTKMAKAWAILKDRSYVIPEDVQEVFTDV
                     CAHRLILKPQARLEGISEKEILQDILKETKPLKDPKRWNA"
     gene            complement(5443..>6064)
                     /locus_tag="ACZPCL_10800"
     CDS             complement(5443..>6064)
                     /locus_tag="ACZPCL_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162991.1"
                     /translation="PVDEDWNYLPEFLEYIDDCTPYTIENIKFTGEERVYTLLGKEVP
                     DHPDRLEKPDINEYVDAWRDRIEEEKNEKEEQAVVLDKIPEVPADVPVDPEVPQDPDQ
                     GNGTTQDPDEPSEDPQDPETPEEPEKPETPTNPDAPETPEIPETPTNPDAPEELDQPQ
                     EPENPDPSEDTDQTENESGGSMEEDKEDTEEKDASSEETSDTEESL"
BASE COUNT         1533 a         1518 c         1163 g         1850 t
ORIGIN      
        1 tcagggcgac cgccgccgcc ttgtccgccg actgtctgag aggcaatggc tttgtctgaa
       61 attgcaaggt cactggactt ggaaagaatg gtatttgctt cccggttcat ctcctgggca
      121 atgaaatcca gtttgcgacc tacactttca ccagataaaa gttcctgtct tgtattttca
      181 atatggcttc ttaaacgaac cgtttcttca tctacacaga tcttatctgc atagatcaca
      241 acctctgcag cgatcctgcc ctcatcaatg gcggtaccgt ttaacagttc tttgacctta
      301 tcttctaatt tctgtctgta ttcctttaag ataacaggag aacgttcctc aataaagtct
      361 acaagtcctg tcatataatc cagcttgcca agaagatctt cttttaaatg agcaccttcc
      421 acctctctgg atgcaacaaa tttctttgct gcctcttcca aagctgcaga aaggatctcc
      481 cacaaatgct cttcatcatc cggaacctgt tccatggtaa ggacttccgg acatctggaa
      541 agggtggaaa cctggatatc gttgcggatc ccgaattcct cttccatttt tttgaaataa
      601 tccatatatt ccccagccag ggctttatta tacttcaggc agagatttcc ttctgtataa
      661 tcctcgtaat tgataaaaag atctaccttt cctctctgga tatatttctt taaaagagta
      721 cggatcgcag cctcaaaata gttaaatttc tttggcatct tgatactcag atctaagtat
      781 ctgtggttta ctgctttcat ttctacagaa atcttgtatt catccgtagc attctcgcat
      841 cttccgaaac cggtcatgct ttttaacata tggaatgcct cactttcatt ttcctatgtt
      901 ctctatataa ttcctatata atctttcata ttataattca tccgtaaatg caagtcaact
      961 gaaaatgcag tagcggaaac ggagcctgtc aaaaattccc atttttttcc tgctgtcacg
     1021 aagctttaac aggaactggt gtacaagtct gatctcatat tcttccaacg gctctccgcc
     1081 atagaaatat ttttccatca gatcatttac cctcagatac agcccatccg gtatttccgg
     1141 caaagtttcc ctgatctttt cctggatctg ttcactttta aatcccggct gcatgtcgat
     1201 ccccaggatc agcagatgct tatagatcat aagaaggaga aattccacac tgggaaggct
     1261 gccattgtca ctgattttcc ggatcctgta ttcataatac attctccgca gttctaatac
     1321 cgcaaaaata aattccaaaa caaacaggat cagcaggatc ataccccagc ctatatctgc
     1381 tgtctttaat tctgtctgct tttgattttc caggaacggt tcacttccag gaaaatgctt
     1441 tttcagattt tctgcttctt ctccctcgga gtccaaagca gctactttgc gcacctgccc
     1501 cggagactgt gccatattaa ggagggcata tgtatcgtaa taaaatcccg gtgtcacatc
     1561 taccggtacc catcccatac catccatata tacttccgcc caggcatgac cgtcagaaga
     1621 agaaaggccg atccagtctc cgtgagaatt ttccgcctgt ttgctggtaa gaagatatcc
     1681 ttctgcatat ctggccggaa tgtgaaaact tctcagtgca agtacggcag cagaagtata
     1741 atatgcggaa tttccttccc gctccccctg taaaaattct tttaaaagat cgtctcccgc
     1801 agtatcttcc tcagaaagat atttattata atacgtattt tcctcaagaa cagtacggat
     1861 ctgctgtaca gccgcataca cacttaaaga tgcttcttcc tcttctttat gaaacagctc
     1921 tttgatcaga ccagacaact gtggatctgc tgtaagataa ttcacataca caaaatcacg
     1981 gtatactgcc tccgactcca gatactgctt ctgttcatct gtaacaggag cctctgtcca
     2041 ggtatctaac cgctgcagtt cccccggcat atccgaagac agctcgctga tctcataacg
     2101 ttttgcgcca aagaaggcaa gggaacggct tccctcatct ctttcattgg tagaattgac
     2161 aactgcctgg gaagaataag gctcatagat atattttctc atggcacctg tatttactac
     2221 ctggatgtgg ttgacccacg gtgcatcctc cggcaaggga tccgtgccgc ttcttccagc
     2281 ttcttcatag gcaatatact gatgttccgg ctgaaaacct cttccgttca gccattgtaa
     2341 aaatccccat ctgtttccgc cataagctgc ttttgccaga ggtttccagc tgtcattctc
     2401 ataaacagaa ccagtaaaac cctttaaata taaaggtttg atctgttctg tctttactct
     2461 gagccttggc tcgtccccct gttctaaaga agctgcctgc gccagactgc cggaaggcat
     2521 attatcctct ccatatcgca gctgttctgt catctgtgct gcgtcctttt tcactttcag
     2581 tactgtctgt gaggtttttc ctcctgatat ccagagcatg caaaaaagga tcagtcctgt
     2641 tacgatcgtc cagccggtac ggcgtccgaa agagccagca tgaaaggcaa acagccatac
     2701 acctgtaatg gaagtgagca taagaacggc tccaaaaagg gaagaacgtc ctaagacgat
     2761 ctccggaaca aacagaacga gggcgatcag gctgatccgg gtacatgcct ttacactgac
     2821 cagataccat aaaagagccg ccagtattaa aagaaagacc agactgtata aaaacagatt
     2881 ctgctctgtt gcctgtacat ttccaaacag acgcactcca tcttcatatt tttcattcca
     2941 gctgtaaata cagatattta aaaagcttaa aaatccgttt aatatctgta ccggtcttat
     3001 aaaggtcagt actgccgcac cggcggcagg aataaaaaat gcccatttct tatctttgga
     3061 agacaagagt ccgtatacag cagtcagaca taaaacaatt tctgctatcc caaaagccgc
     3121 agcctcaaaa acaaggtcga tcgccgcaat cttaaaccag gataaaatgc ttcctgccac
     3181 cagtatcatg cacaatggca gcgcgtttaa aatatccctg ttcttattat ttttccctga
     3241 tgatatttcc tgtatcacac ccgccatagt ttatgttttg tcttcctttc tacacaatga
     3301 tattctgcgt gtctgtggtt aatttttcta atgggatctc tgttaatacg cacatacctc
     3361 tttctgtagt acgtatctct ccctcttcat ctaaaataca cacagctgtt acacttacat
     3421 cttttgccag ttcataaacc tcatccggac aggttcctgc agtaaaatac aggatcctgc
     3481 ggtaattact gtgagatttt tccgcaataa ggctctttaa gcccatcccc cgctctttct
     3541 gaagggaaag gttcatccag gtatccatgg tattatcata ttcctgctgt cctgtgatct
     3601 ggttcagata tattttattt ccggcccaca tgcccagata atgagggacc ttatgttcca
     3661 tcagtttttc actgactgct cctgataaaa catccggttc atatgtgacc tctccagaag
     3721 taagacctgc gtcaaacatc accagaatat cataactgga cgtatcactt ggttctctta
     3781 ccagaacggt gtcaaacttt tctgaaagtt tccagtggat cgttcggata ctgtctcccg
     3841 gctgatattc acgcagctca aaaacttccg ttgcatcata acctttttta ctcagcgtct
     3901 gctgtccttc attctggcgt cctttctgat ttccggatgc cacaggacgc atggatattt
     3961 tctccggata aaccgtcaga tgctgtacct gatgaaattc tgtctttata cagtttaagc
     4021 caaaaatatc atagcagcgc acattactaa ggctgaggat cgcgcctccg cacatcttcg
     4081 gttcccaagg gatcgatata ccaaaagaac gtttattgat gtcaagggct aacgggatat
     4141 tcctgttgac ctggaacatt ttattatcat aggaaagatc tgcctggatc ctggctgctg
     4201 ccagacaccg ccatctgctt ttcacagaga tatcgatctc tattgtctgt ccgagacggc
     4261 aggcagagcg ggtatgcatt tccagctcca ttccattcgt ctcaagacgt aaaaagaaac
     4321 gtaagaccac aggcaataaa atttctatta taaatacacc ggctgtaaaa taagaaccgc
     4381 ttaagaaaaa cagaacagct gttgccacta ctgctgtgcc gaagccgatc ctgatccttg
     4441 tcatgcattc cacctcttag ggtctttcaa aggttttgtt tcttttaata tatcctgaag
     4501 gatctctttt tctgagattc cctctaaacg ggcctggggc ttaaggatca gtctgtgggc
     4561 gcatacatcc gtaaatactt cctgtacatc ctccgggatc acatagctgc ggtcttttaa
     4621 gattgcccat gcctttgcca tcttagtcag tgcccagaca ccacgtgggc tgattaccag
     4681 ttctactaaa ggatgttctc tggttgcctc acaaaggcgg atcagataat aaaggatatc
     4741 atcactgatc ttaagagcag aaagatagtt ctggatctcc agaatgtttt ctgcctgtgc
     4801 agctacctgg atctcttcca gagggttgga atatcttctg gcctttaaga tcttgatctg
     4861 atcctctgct gaaggatatc caatggaaag acgaaccata aaacggtcta actgggaact
     4921 tggaagtgcc tgggttcctg ttgtacctaa agggttctgg gtagcaatac agataaacgg
     4981 agtaggtaaa ggtcttgtta caccatctac tgttaccctc cgctcttcca tggcttccag
     5041 aagagcagac tgtgttttag gagaagtacg gttgatctca tctgccagca gcagattgca
     5101 catagcagcg ccctcatgat actcaaactg ttcattctgg cggttgtaca tggtaaaacc
     5161 tgtaatatct gacggcaggg tatctgttgt aaactgtatt ctcttgtaat ccagtcccag
     5221 tactcttgaa aaggcaaggg caagggtggt ctttcctact ccggggctgt cctcaaggag
     5281 aatatgtcct cctgcgtaaa tcgtggcaag aacacgccgt ataatgtcgt cttttcccat
     5341 aaaggctttt ttcacttcct gaatgacgtg ttcagactgt tccagtatca taacaaatgc
     5401 ttctcctctc tcttttgcgg tttttccgtt ctatagcggt ctttataaac tttcttctgt
     5461 atctgaagtt tcttctgaag acgcatcttt ttcttctgtg tcttctttat cctcttccat
     5521 cgatccacct gattcatttt ctgtctgatc cgtatcctct gacggatccg ggttttccgg
     5581 ttcctgtggc tgatccagct cttctggtgc atccggattg gttggtgtct ccggtatttc
     5641 tggtgtttcc ggcgcatctg gattggttgg tgtctccggt ttttccggtt cttccggtgt
     5701 ttccggatcc tgtggatctt ctgatggctc atccggatcc tgtgtggttc catttccctg
     5761 atccgggtcc tgaggaactt ccggatctac cggaacatcg gcaggaactt ccggtatttt
     5821 atccagaaca actgcctgtt cctctttttc attcttttct tcctcgatcc ggtctctcca
     5881 ggcatctaca tactcattga tatccggttt ttccagacgg tccggatgat ccggtacctc
     5941 tttaccaagg agagtataaa ctctttcctc accagtgaat tttatattct caatcgtata
     6001 cggagtacag tcatctatat actctaagaa ttccggcagg tagttccagt cttcatctac
     6061 aggg
//
LOCUS       DBMDUA010000163         6017 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5289_length_6017_cov_2.205206, whole genome shotgun sequence.
ACCESSION   DBMDUA010000163 DBMDUA010000000
VERSION     DBMDUA010000163.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5289_length_6017_cov_2.205206"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="ACZPCL_10805"
     CDS             <1..401
                     /locus_tag="ACZPCL_10805"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1162992.1"
                     /translation="AIVHGVLKDDTGTIDAPIGRHPTDRKKMSINYNHGKNAVTHYKV
                     LKRFRQYTHVECRLETGRTHQIRVHMASIGHPLLGDVVYGPGKCPIAGLEGQTLHAGI
                     LGFIHPRTGEYMEFTAPLPEYFEKLLNTLP"
     gene            561..1199
                     /locus_tag="ACZPCL_10810"
     CDS             561..1199
                     /locus_tag="ACZPCL_10810"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1162993.1"
                     /translation="MSGNLIITIGRQSGSGGKKIGEMLAEKLGVKCYDKELLAQAAKH
                     SGLCEELFEKHDERPTSSFLYSLVMDSYSMGYTATGYSDMPINHKIFLAQFDTIKKMA
                     DEGPCVIIGRCADYALEDYPNVVSVFITGDEADKIKRVSELYNVDEAKAKDLMIKTDK
                     RRSSYYNYYSNKKWGDPKSYDMCINSSAVGQEGAVDIIMELAKKKQEWKKQK"
     gene            complement(1268..3097)
                     /gene="glmS"
                     /locus_tag="ACZPCL_10815"
     CDS             complement(1268..3097)
                     /gene="glmS"
                     /locus_tag="ACZPCL_10815"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR1162994.1"
                     /translation="MCGIIGYTGPLEADNILLNGLAGLEYRGYDSAGIAYFGNDSKIH
                     LIKTVGKVAALKELVKQESDCSSHCGIGHTRWATHGGVTTENAHPHRQGLVTLIHNGI
                     IENYHQLTEEYDLADHLLSQTDSEVAAGVLNALYERMDHDPLLSIRHFVKELQGSYGF
                     CIMFEDRPGEIYAVRNVSPLVAAYTHSGAMIASDLTALIAYTKEYFVVPEGNIVRLTP
                     YKVRVYDMELNRVEPDMMEVNWNVDAAMKNGFPHFMLKEIHEQPDALKNTILPRLSKG
                     LPDLTDDGIPDFILKDCSRIHVIACGTAMHAGMVARALIEPGIRIPVTVSIASEFRYE
                     TPLIDEKTLVIVISQSGETIDTLAAMRLAQSYHAPVLAIVNVKGSTIARESDYVLYTH
                     AGPEIAVASTKAYSVQLAALYLIYCRMALVRGVYSPKQAEDFMKELLDAIPAMEAMIN
                     RRDEIKNIVTHIISKNDAFFIGRGLDYAFSLEGALKLKEISYIHAEAYAAGELKHGTI
                     ALISEQVPVIAIATQEHIFAKTISNVREVKARGAFVILLVKDSAVIEDDIADVQIRIP
                     DIADSFTVFPIAAILQLIAYYASVGKNLDVDQPRNLAKSVTVE"
     gene            3802..5547
                     /locus_tag="ACZPCL_10820"
     CDS             3802..5547
                     /locus_tag="ACZPCL_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1162995.1"
                     /translation="MRLHENKEGDELQRLRARRQGTGRNYKNRPRQTKTVDHTYDEDY
                     YEEEDYDYEYEEEPDPNGLEVPFENDNRRSAGVSHRYSEYSNQSRGKRKTSNRYSTSG
                     TTGNVEASASSGKTGKTGRNNPGKTSRKRKKAKWPVILLSLLVVLCVYGGWSFLHRAT
                     GYWNIAVFGVDSRDGNTKNALADVQLICSINQETGEIKLVSVYRDTYLKIDSKGTYHK
                     INEAYFKGGHKQAVSALEENLDVKINDYATFNWKAVAQAINVLGGVDIEITQPEFKYI
                     NVFITETVNSTGIGSTQLASAGPNHLDGVQAVAYCRLRLMDTDFQRTERQRKVISLAL
                     EKAKQADLATRTNLVKAILPQISTSVGVDDVLPMAKNASKYHIGETAGFPFAQKTKKM
                     GKMDCVISVTLESNVVTLHQFLYGEDALYSPSSTVKKISDHIAEESGFYEGGKAAPTS
                     SGSGKSDSKSQSSGTSKNNAPAQTAAQTAAEESSEAEETTEETGDTTDKTAAEESTAA
                     IIEKLDDSKTEKETTSASEGGPGTVKPKETEKAETAADESSKGPGVQKTTENSENDNA
                     SGENTGNTDPIGPGV"
     gene            5658..5915
                     /locus_tag="ACZPCL_10825"
     CDS             5658..5915
                     /locus_tag="ACZPCL_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1162996.1"
                     /translation="MISCRYVISTNHGLHAANAMSLSRAASDYQCKITLESAKGVADC
                     KNVLSLMSLGAKQGESLILTADGPDEKLAMGYLSGILRTIL"
BASE COUNT         1843 a         1239 c         1359 g         1576 t
ORIGIN      
        1 aggccattgt ccacggtgtg ttaaaagatg atacaggtac cattgatgct cctattggac
       61 gtcatcctac agaccgtaaa aaaatgagca ttaactataa tcatggaaaa aatgcagtga
      121 cccattataa ggttctgaaa cgtttccgcc agtatacgca tgtggaatgc cggctggaaa
      181 caggacgtac ccatcagatt cgtgtgcata tggcaagtat cggacatcct cttttgggag
      241 atgtggttta cggaccagga aaatgtccta ttgccggact ggaagggcag actcttcacg
      301 ctggaatttt aggatttatc catccacgta caggagagta tatggaattt acagccccgc
      361 ttcccgaata ttttgaaaag cttttgaata cattacctta ataacctatt tgtccgacat
      421 ttgtcggaca tttttatatt tctatatact tttctgaatt tttgagttat aatacaggta
      481 taaaagataa atgctagaaa aaatataacg aaaggcgaga tgaacagaca tcagacgcta
      541 agcggaaagg ggtaggtttt atgtcaggaa atcttattat caccatcgga agacagagcg
      601 gaagcggcgg caagaagata ggagagatgc ttgctgaaaa actgggagtt aaatgctatg
      661 ataaggagct ccttgcccag gctgcaaagc acagcggact ttgtgaagaa ttatttgaaa
      721 aacatgacga gcgcccaacc agcagttttc tttattctct ggtaatggat tcttattcta
      781 tgggttatac agctaccggt tattcggata tgccgatcaa tcataagatc ttccttgcac
      841 agtttgatac cataaagaaa atggctgatg aaggtccttg tgttattatc ggacgttgtg
      901 ctgattacgc cctggaagat tatccaaatg tagtcagtgt atttatcacc ggagatgaag
      961 cagacaagat caaacgtgtc agtgagcttt acaatgtaga tgaagcaaaa gcgaaagatc
     1021 tgatgatcaa aactgacaag agaagatcca gctactacaa ttactattcc aataaaaaat
     1081 ggggcgatcc aaagagctat gatatgtgta tcaacagcag cgctgtagga caggaaggtg
     1141 ccgttgatat tatcatggaa ctggcaaaga agaaacagga atggaaaaag cagaaataaa
     1201 actgctacat aaaacatata gaatattgta aacggaatac tggggatttt tccccagtat
     1261 tcttatttta ttctacagta acggattttg ccaggtttct tggctggtcc acatccaggt
     1321 tctttcctac agatgcataa tatgcgatca gctgtaagat agcagcgatc gggaacacag
     1381 taaagctgtc tgcaatatcc gggatacgga tctgaacatc ggcgatatca tcttcgatca
     1441 cagcagagtc ttttacaagg aggatcacaa aagcgcctct tgcctttact tcccgtacat
     1501 tggagatggt ttttgcaaaa atatgctcct gggtagcaat ggcgatcaca ggtacctgct
     1561 cggagatcag tgcaatggta ccatgtttta actcgccggc tgcgtatgcc tctgcgtgga
     1621 tataagagat ttcctttaat tttaaggctc cctctaagga gaaggcgtag tcaagacctc
     1681 ttcctataaa gaaggcgtca tttttggaaa taatatgagt aacaatattt ttgatctcat
     1741 ctctgcggtt gatcatagct tccatagcag ggatggcatc taagagttct ttcataaaat
     1801 cttcagcctg ttttggactg tatacgcctc gaacaagagc catacggcag tagatcaggt
     1861 ataatgcagc cagctgtaca gaatatgctt ttgtgctggc taccgcgatc tcaggacctg
     1921 cgtgtgtgta aagaacatag tcactttctc ttgcaatggt ggagcccttt acattgacaa
     1981 tagccaggac cggtgcatga taagactggg caagacgcat agctgccagg gtatcaatgg
     2041 tctcaccgga ctgggagatc acgatcacca gggtcttttc gtcaataaga ggtgtctcat
     2101 aacggaattc ggatgcaata gatactgtga ccggaatacg gataccaggt tcgatcagag
     2161 ctcttgccac cattcctgcg tgcatggcgg ttccgcaggc gatcacatgg atgcggctgc
     2221 agtcttttaa gataaaatcc ggaataccgt catctgtcag atccggaagt cccttggaaa
     2281 gacggggaag aatggtattc tttaacgcat ccggctgttc gtggatctct tttaacataa
     2341 agtgtgggaa accatttttc atagctgcat ccacattcca gtttacttcc atcatatccg
     2401 gctcgacacg gtttaattcc atatcataaa cacgtacctt gtaaggagtc agacgaacga
     2461 tatttccctc aggcactaca aaatattctt ttgtataagc gatcagagca gtaaggtcag
     2521 aggcgatcat tgctcctgag tgggtataag ccgctacaag gggacttaca ttgcgtactg
     2581 cataaatctc acctggacgg tcttcaaaca tgatacagaa gccgtaggaa ccctgaagct
     2641 cttttacaaa atgacggatg gacagaagcg gatcatgatc catacgctca taaagggcat
     2701 ttaacacacc ggcagctact tcactgtctg tctgtgaaag cagatgatcc gccagatcat
     2761 attcttctgt aagctgatga taattttcaa taatgccgtt gtggatgaga gttaccagtc
     2821 cctgacggtg tggatgcgca ttttctgttg taacaccgcc atgggttgcc caacgggtgt
     2881 gtccgatacc acagtgggaa gagcagtcgc tttcctgttt taccagttct tttaatgccg
     2941 ctactttacc gactgttttg atcagatgga tcttggagtc atttccaaaa taagcgatac
     3001 cggcgctgtc ataacctctg tactccagac cagccagtcc atttaaaagt atgttatctg
     3061 cctcaagagg ccctgtatat ccaataattc cgcacataat acaattcctt tcccagagca
     3121 gccgtaaata tccgactatt gcgtacgcaa agaaaaggga tatgatccct ctgatatatg
     3181 actgtctgct tccattttaa ccttgtttta atttcgtttt gacgttttgc ttttcgtatc
     3241 tgttctgtcc ggttatacca gctttgtttt gcagttaccc gcatattttt ctggtataaa
     3301 acacgcccgg actgcatggg agagcacccg ccgaatatgt tcgattctct ctccacctcg
     3361 ttaacctgtg tcagcagtaa gttattattc attcatccgt tactgttcac gacccgaccc
     3421 aggtgcatgg cgctatagct ttactattat ctttgtttcc tccaaaaaca aatcccgtat
     3481 attatatctc atatttacaa tatgcgtcaa ctcttaattt tgccgctatt atgctacctt
     3541 tttcccaacc cgccttaaag tttctgtaag agtttcacac tgtattttgc taaaatggta
     3601 tgttataatg tgatgggcgc agcgaaaagt aagcgacacc gcaggtggca tttatttttc
     3661 gcaagctcat caacgaacga accgtctgcg tcagcagaca gtggagcgcg tcagcgcggg
     3721 aaacctgagt gaaccaaacc gccgggaaaa ataaacggta tttattttta gtaagaaaga
     3781 agagaataga ggtaaccgat catgcgttta catgaaaata aggaaggtga cgaactgcag
     3841 cgtttgcggg cccgccgcca gggaacagga agaaactata aaaaccgtcc aagacagaca
     3901 aaaactgtcg atcatacata tgacgaggat tattacgaag aagaagatta cgactatgaa
     3961 tatgaggaag aaccggatcc gaacggactg gaagttcctt ttgaaaacga caaccgccgc
     4021 agtgcaggtg tttctcaccg atatagcgaa tatagcaacc aaagcagggg aaaaagaaaa
     4081 accagcaacc gttatagcac atctggaact acaggaaatg tggaagcttc tgcatcatca
     4141 ggcaaaacgg gtaaaaccgg cagaaacaat cctggtaaaa catccagaaa gcggaaaaag
     4201 gcaaaatggc cagttattct tttaagcctt ttagtggtgc tttgtgtata tggaggctgg
     4261 tctttccttc acagagctac gggatactgg aatattgcgg tatttggtgt agacagccgt
     4321 gacggaaata cgaaaaacgc cctggcggat gtccagctga tctgcagtat caatcaggaa
     4381 acaggagaaa taaaactggt aagcgtttac cgtgatacat atttaaagat cgactccaag
     4441 gggacctacc ataagatcaa cgaggcttat ttcaaaggcg gtcataaaca ggctgtatct
     4501 gccctggaag agaacctgga tgtgaagatc aacgattacg ctacatttaa ctggaaagct
     4561 gttgcacagg cgatcaatgt tttaggcggt gtggatatag agatcacaca gccggaattt
     4621 aagtatatta atgtctttat tacagaaact gttaattcta ctggcattgg ttccacacag
     4681 ctggcaagtg caggtccaaa ccatttagac ggtgtacagg ctgtagctta ctgtagattg
     4741 cgtctcatgg atacggattt ccagcgtaca gagcgtcaga gaaaggttat ttcactggcc
     4801 cttgaaaaag caaaacaggc agaccttgct acccgtacaa atctggtaaa agcgatcctc
     4861 ccacagatct ccaccagtgt gggtgttgat gatgtacttc caatggcaaa gaatgcaagt
     4921 aagtatcata ttggtgaaac agcaggcttc ccatttgcac agaaaacaaa gaaaatggga
     4981 aaaatggact gtgttatctc tgtaacactg gaaagcaacg tagttaccct tcatcagttc
     5041 ctgtatgggg aagacgccct atacagtcct tcttctacag taaagaaaat cagtgatcat
     5101 attgctgaag aatcaggttt ttatgaaggt ggtaaggcgg ctccgacttc ttccggttcc
     5161 ggaaaatcag acagcaagag ccagtcatcc ggaacaagta aaaataatgc tccggcccag
     5221 actgctgcac aaacagcagc tgaagaaagt tctgaagcag aagaaacaac agaggagacc
     5281 ggcgatacaa cggataaaac agcagcagaa gagagtacag cagctattat tgaaaaactg
     5341 gacgactcca aaacagagaa agaaactacc agcgcatcgg aaggcggccc aggtactgta
     5401 aagccaaaag aaacagaaaa agcggaaacg gcagcggatg agtcctccaa aggacctggt
     5461 gtccagaaaa caaccgaaaa ttcagaaaat gacaatgctt ctggtgaaaa tacaggaaat
     5521 acagatccga taggtccggg agtttaattt ccaatattgt atttgtcaaa agtctgtgtt
     5581 aaaatgcttt caaaagataa ataaataaca aataaacgat tttaaaacaa cattattgcg
     5641 aaagaagaga tgccattatg atcagctgcc gttatgttat ttctaccaat cacggccttc
     5701 atgcggccaa tgccatgagc ttaagccgtg ccgcttccga ctaccaatgc aagatcacac
     5761 tggaaagtgc caagggagtt gccgactgca aaaacgtttt atccctgatg agccttggcg
     5821 ccaaacaggg agagtccctg atcctgactg ccgatggacc ggatgagaaa ctggctatgg
     5881 gatatttaag cgggattttg agaacgattt tgtgatttgc tttaaatgat tttaaatgat
     5941 ttttaaatga tattgatact tgagaataat gtaaatgatt aattgtagta tataatcgat
     6001 aatagaatgg attgatt
//
LOCUS       DBMDUA010000164         5944 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5352_length_5944_cov_2.700667, whole genome shotgun sequence.
ACCESSION   DBMDUA010000164 DBMDUA010000000
VERSION     DBMDUA010000164.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5944)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5352_length_5944_cov_2.700667"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..536)
                     /locus_tag="ACZPCL_10830"
                     /pseudo
     CDS             complement(1..536)
                     /locus_tag="ACZPCL_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_343251044.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            829..1725
                     /gene="ispE"
                     /locus_tag="ACZPCL_10835"
     CDS             829..1725
                     /gene="ispE"
                     /locus_tag="ACZPCL_10835"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR1162997.1"
                     /translation="MITHLRLKAYGKINLALDVLGRRDNGYHDVRMIMQTVGLHDRIE
                     LYRTKEPGIRLETNLFYLPNNEQNLAYRAAALLMEEFGVKEGVSIKLKKFIPVSAGMA
                     GGSTDAAAVLFGVNKMFGLGLSIKELMERGVKLGADIPYCLMRGTALSEGIGEELTPL
                     PQIPQCQVLIAKPGISVSTKVVYESLDAMELKQEDHPDIDGMIEAINRQNIHEIAGKF
                     GNVLELVTAGKYPVIGEIEQVMKEEGAVNAMMSGSGPTVFGLFTNPQAAQNAYEKLRY
                     GDASKLAKQVYLTNFFHVKRSR"
     gene            1799..2506
                     /locus_tag="ACZPCL_10840"
     CDS             1799..2506
                     /locus_tag="ACZPCL_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1162998.1"
                     /translation="MENNFKVNMNEYLPLRDVVFNTLRQAILKGELAPGERLMEIQLA
                     EKLGVSRTPIREAIRKLELEGLVLMIPRKGAEVAKISEKSLRDVLEVRRSLEELAIEL
                     ACQRMTDEEIEQLGERQNDFKNAINKGNAMNIAETDEAFHDVIYLGTGNDKLVQILNN
                     LREQMYRYRLEYIKDEDKRQILIVEHEHILAAIKARNIAEAKNAAREHIDNQEITVSK
                     NIKEQEDIMPVRGRGRR"
     gene            2534..2761
                     /locus_tag="ACZPCL_10845"
     CDS             2534..2761
                     /locus_tag="ACZPCL_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1162999.1"
                     /translation="MEENKVEDKTEKTFKERYLAGEIEFEEIDRYISRWNNSDDERTL
                     AVYLGLNADEEDVWIDESDEALQEMLDKQKA"
     gene            complement(2764..3387)
                     /locus_tag="ACZPCL_10850"
     CDS             complement(2764..3387)
                     /locus_tag="ACZPCL_10850"
                     /inference="COORDINATES: protein motif:HMM:NF021093.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HIR1163000.1"
                     /translation="MKSVSYRHKWLICSLACFFTAFLIAVSANTCKQEADASRIAPYV
                     LRFHILAESNSKTDQELKLKVRDQVLSYIRENAPDHAGKEELASWLLSHKEEVVFKAE
                     QTLADSDCEADVDLNLSHDHFPARTYGNAFFPCGYYDAARITIGKGKGRNWWCVLYPG
                     LCFTNSYHTDIDETASESYSLVFETAPRPELHLGLLDAAKQLFKQKA"
     gene            3533..4591
                     /locus_tag="ACZPCL_10855"
     CDS             3533..4591
                     /locus_tag="ACZPCL_10855"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR1163001.1"
                     /translation="MERKTAAVIFGGQSSEHEVSCMSAANVIDRINKEKYDLLLIGIT
                     LDGHWIKTESVEEIRSGEWRNGKVSAAILPDATKKCAILMDGDKVTEVKLDLVFPVLH
                     GLHGEDGTIQGLLELAKIPYVGCGVLASAVGMDKLYTKIIVNNLGIRQADYEPVMSEQ
                     LADMDAVTARIEAHFAYPVFIKPSNAGSSKGVSKAENREELEAGLKEAAKHDRKILVE
                     ETIIGREIECAVFGGGKEEVKASGVGEILAAADFYDFDAKYYNAESKTVTDPELPGNA
                     TEEVRKAAVAIFKAVDGYGLSRVDFFVKEDGEVVFNEINTMPGFTAISMYPMLWEARG
                     VNKEELVDKLMSHALERF"
     gene            4628..5050
                     /locus_tag="ACZPCL_10860"
     CDS             4628..5050
                     /locus_tag="ACZPCL_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiB family protein"
                     /protein_id="HIR1163002.1"
                     /translation="MTKDVLITIRGVHTMDEEDNDVEMIVRGDYYQKNGKHYVLYEEL
                     LEGFKEPVKNIIKITPTSMDITKKGTATTHMLFEKNRKNISCYSTPLGEMVVGIQANH
                     FAVDEQADSLKVNVDYSLDINYEHMSDCRILVAVQSCQ"
     gene            complement(5148..5597)
                     /locus_tag="ACZPCL_10865"
     CDS             complement(5148..5597)
                     /locus_tag="ACZPCL_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163003.1"
                     /translation="MLNTVLNILLVILIIAAVILAILYFLGNKLQKRQLEQQQMLDAA
                     AQTVSLLVIDKKKLKLTQAGLPKMVVDQTPKYMRWTKVPVVKAKIGPKIVTLIADARV
                     FECLPVKTEAKVVLSGIYITQIKSVRGGSIPTPPKKKGFFARFRKDA"
     gene            complement(5746..>5944)
                     /locus_tag="ACZPCL_10870"
     CDS             complement(5746..>5944)
                     /locus_tag="ACZPCL_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163004.1"
                     /translation="RILMADWIQIKVFLLFSIDVSPLFSYLVAAAFSGIFLSFSAPFS
                     TAVVLGNRASANGRNKPRTPQ"
BASE COUNT         1896 a         1113 c         1451 g         1484 t
ORIGIN      
        1 aaagataaaa gaatgtcctg tctccatttc gtaaaacttt ttatgaagcg gtgtttcgat
       61 ttgtgaatag cacgataaag tttagtgtca atttcacggt gcctcgcata gagaccatag
      121 tagcggatca ttttaaaatg tttttccggt atatgttgga taagaagttt aataaactcg
      181 attgccggaa tcgtacgttt aacataagta ttatcttcgt gacgattata gtgaaaagta
      241 acatttttac catcgtaaga atcaatccgt tttaaggcaa taaccggacg gccaagatag
      301 cggccaatgt atttaatgac agtattaggg tcgcagagat taggcttggc atagacataa
      361 aagccgtttt tgtccttgtg atagatataa gctttaatct ttttgaagga aggtccgatg
      421 tgctgttcca tttcattgag taaagcggtt tgaaaagcat tgcgtagata agtgtaattg
      481 aaatgtttta ccggccgcca aatagcattg tcagaaaaac cgccttccga aagaatgggg
      541 gatataaaac agtctggccc tttgagtatg cgaaggaaaa gatccagtat ccggcacggt
      601 acagcaggtg atcttcagct taattttttt taatattttt ttaatgtttt cactcttgaa
      661 aaatgagaca aattaatgta tacaatatta tgtattatga catgtcgcca ttttttgaag
      721 aaaaatgagt ggattacgaa tgttttagga ttgcgagaac acaaagtgcg gagcaatccg
      781 ttggtatcag tggaattaac ggaattgaga aaaaagggga tagcagctat gatcacacat
      841 ttacgcctga aggcatatgg aaagataaat ctggcgctgg atgtactggg gagaagggac
      901 aacgggtatc atgatgtccg catgatcatg cagactgtag gacttcatga ccggatcgaa
      961 ctttacagga caaaggagcc tggtatccgt ctggagacca acctgtttta ccttccaaat
     1021 aatgaacaaa atctggctta ccgggcagcc gctcttttaa tggaagagtt tggtgtaaaa
     1081 gaaggtgttt ccataaaact gaaaaagttc attcctgtat cagctggaat ggccggaggc
     1141 agtaccgatg cagctgcagt cctttttgga gtcaataaaa tgtttggcct gggcttaagt
     1201 ataaaagaac tgatggaacg tggcgtaaag ctgggagcag atatcccata ctgcctgatg
     1261 cgtggaaccg ctctttccga aggaattggg gaagaattaa ctccgttgcc tcagatcccc
     1321 cagtgtcagg tattgatcgc aaaaccgggg atcagcgttt ctacaaaagt ggtttatgag
     1381 agcctggatg ccatggagct aaagcaggaa gatcatccgg atattgacgg catgattgaa
     1441 gcgatcaacc gccagaatat ccatgagata gccggaaaat tcggaaatgt gctggaactg
     1501 gtaacagccg gtaaatatcc ggtgatcggt gagatcgagc aggtgatgaa agaagaaggg
     1561 gctgttaatg ccatgatgag cggaagcggt ccaactgtat ttggtctgtt taccaatcca
     1621 caggcagcac agaatgccta cgaaaagctt cgctatggtg atgcttccaa actggcaaag
     1681 caggtatatc tgaccaactt ctttcatgta aagcggagca gatagaaaca agcagatcgt
     1741 aaacataaaa atatagacaa acagacatag ataaaacaca gaaaggaatg gtcttgagat
     1801 ggaaaataat tttaaagtca atatgaacga atatctgcct cttcgcgatg ttgtatttaa
     1861 tacactgcgt caggctattt taaaaggaga actggctccg ggagaaagac tgatggagat
     1921 ccagctggca gaaaaattag gtgtaagccg tacaccgatc cgtgaagcca ttcgtaaact
     1981 ggaactggaa ggtcttgtac ttatgatccc aagaaaaggc gctgaggttg caaagatctc
     2041 tgaaaagagc cttagggatg tgctggaagt tcgccgttcc ctggaagaac tggctattga
     2101 actggcttgc cagagaatga ccgatgaaga gatcgaacag ttaggggaac gccagaacga
     2161 ctttaagaat gccattaata agggaaatgc catgaacatt gcagagaccg acgaggcatt
     2221 ccatgatgtg atctatctgg gaaccggaaa tgataaactg gtacagatct taaataattt
     2281 aagagagcag atgtatcgct atcgtctgga atatatcaag gacgaggata aacgccagat
     2341 cctgatagta gagcatgaac atatcctggc tgcgatcaaa gcccgcaata ttgcagaggc
     2401 aaagaatgca gcaagagaac acattgataa tcaggagatc actgtttcca agaatatcaa
     2461 ggagcaggaa gatatcatgc ctgtccgcgg ccgtggaaga agatagcgga aatggcattg
     2521 gaggagaaga taaatggaag aaaataaagt agaagataag acagaaaaga ccttcaaaga
     2581 gcgctatctt gcaggagaga tcgaatttga agagatcgat cgctatatca gccgttggaa
     2641 caacagcgat gatgagcgta cactggctgt ttatctgggg ttaaatgcag atgaagagga
     2701 cgtatggatc gatgaaagcg acgaagctct tcaggagatg ttggataagc agaaagcctg
     2761 aaattaagcc ttctgcttaa aaagctgttt tgcagcatct aaaagtccaa ggtgcagttc
     2821 cggccttgga gcagtttcaa agaccaggga atagctttca gaagctgttt catctatatc
     2881 tgtatgataa gaatttgtaa agcaaagacc gggatagagg acgcaccacc agttgcgtcc
     2941 ttttcctttg cctatagtga tccgtgcagc gtcatagtaa ccgcagggaa agaaggcatt
     3001 tccataggtc cttgctggaa aatggtcatg gctcagatta agatccacat ccgcttcaca
     3061 gtcggagtct gccagggtct gttctgcctt aaaaacgacc tcttccttat gagaaagaag
     3121 ccaggaagcc agctcttctt ttccggcatg atccggggca ttttcacgga tataggaaag
     3181 aacctggtcc cggactttta attttaattc ctggtccgtt tttgaattgc tttccgctaa
     3241 aatgtgaaaa cgcagcacat aaggggctat acggctggca tctgcctcct gcttacaggt
     3301 gttggcagaa actgcaatga gaaaagcagt aaaaaagcag gcaagagaac aaataagcca
     3361 tttgtggcga taagatacag atttcatgga ctaaaacttc ctttctggat tttgagagaa
     3421 catattttct atttgtattt attgccacat atgctatact gtaaacggta aaatctaaga
     3481 aaagaaagat tttaaagatt tctggaaaag ggataagtga ggagatataa aaatggaaag
     3541 aaaaacagca gcagtcattt tcggcggaca gtcatcagaa catgaagtat cctgcatgtc
     3601 tgcagccaat gtgatcgacc gcatcaacaa agaaaaatat gacctgcttc ttattggtat
     3661 tacattagac ggacactgga tcaagacaga gtctgtagaa gagatcagaa gcggtgagtg
     3721 gagaaatgga aaagtttccg cagctatctt accagatgct acaaagaaat gtgcgatcct
     3781 tatggatgga gataaagtaa cagaagtaaa attggacctt gtattcccgg tacttcatgg
     3841 acttcacggc gaagacggaa ccatccaggg tcttttagaa ctggcaaaga tcccatatgt
     3901 aggctgcggt gtactggcat ctgcagtggg catggataaa ctgtatacaa agatcattgt
     3961 aaataactta ggcatccgcc aggcagatta tgagcctgtt atgagcgagc agttagcaga
     4021 tatggatgct gtaacagcac gtattgaagc acattttgca taccctgtat ttatcaagcc
     4081 gtctaacgca ggttcctcca agggagtcag caaggcagaa aacagagaag aactggaagc
     4141 cggcttaaaa gaggctgcaa agcatgacag aaagatcctg gtagaagaga ctattattgg
     4201 ccgtgagatc gaatgtgctg tattcggcgg cggaaaagaa gaagtgaagg cttccggtgt
     4261 aggcgagatc ctggcagcag cagactttta tgattttgac gcaaaatatt acaatgctga
     4321 gtccaagacg gtaacagatc cagaacttcc tggaaatgcc acagaagaag taagaaaagc
     4381 agcagttgct attttcaagg cagtagacgg atacggactt tcccgtgtag atttctttgt
     4441 aaaggaagac ggagaggtag tatttaacga gatcaatacc atgcctggat ttacagccat
     4501 cagtatgtat ccaatgcttt gggaggcaag aggcgtcaac aaagaggaat tagtagacaa
     4561 gctgatgagc catgctttag agcgtttcta agatcacagg tttacgtaaa aagaaaagag
     4621 gagttacatg acaaaggatg tactgatcac catccgcggt gtccatacca tggatgaaga
     4681 ggacaatgat gtggagatga tcgtccgggg agattactac cagaagaatg ggaaacatta
     4741 cgttctctac gaagaactgt tagaaggttt taaagaaccg gtaaagaata tcatcaagat
     4801 cacgccaacc tctatggata ttacaaaaaa gggaacagcc actacccaca tgctgtttga
     4861 aaagaaccgg aagaatattt cctgctacag caccccttta ggtgaaatgg tagtagggat
     4921 acaggccaat cactttgcag tagatgagca ggcagacagc ctgaaggtga atgtggatta
     4981 ttccctggat attaattatg agcatatgtc ggattgccgc attttagtag ctgtgcagtc
     5041 ctgccagtag aaagagaggg cgccccgcat ccgcagggaa atgcacctca tcaaaacaaa
     5101 aaacagccgc tcccatccgg gagccggctg tttttttacg ccccatatta tgcatcttta
     5161 cggaatcttg cgaaaaatcc ctttttcttt ggcggagttg gaatggaacc tccgcggaca
     5221 ctcttgatct gggtgatgta gataccgctt aaaactacct tagcttctgt tttaacagga
     5281 aggcattcaa acactcttgc atcagcgatc agtgtaacga tctttggtcc gatctttgct
     5341 ttcacaaccg gaaccttggt ccagcgcatg tactttggag tctggtcaac taccattttt
     5401 ggaagacctg cctgagtcag cttcagtttc ttcttatcaa taacaagaag agatacggtc
     5461 tgtgctgcgg catctaacat ctgctgctgt tctaactggc gcttctgaag cttatttcca
     5521 aggaaataca ggatagccag gatgacagct gcaatgatga ggatcaccag cagtatgttt
     5581 aaaacggtat ttaacataag gtatacctcc gaactgtagt atagttcccg tgggcttgat
     5641 tagacatgcc ccaagacatt ctaatatagt atatcatttg tggtaaaaaa aagcaagaaa
     5701 aaattcttga caccttgaca aatatatgtt attatggaga gtgcattatt gtggcgtcct
     5761 gggcttattg cgcccatttg ctgacgcccg attaccgaga acgacagcag tggaaaaagg
     5821 ggcagaaaaa gaaagaaaaa tccccgaaaa agccgctgca accaaataag aaaacagggg
     5881 tgaaacgtca atggagaaaa gcaggaatac ttttatctgt atccagtctg ccatcaaaat
     5941 acgg
//
LOCUS       DBMDUA010000165         5940 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5355_length_5940_cov_3.000171, whole genome shotgun sequence.
ACCESSION   DBMDUA010000165 DBMDUA010000000
VERSION     DBMDUA010000165.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5355_length_5940_cov_3.000171"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            290..751
                     /locus_tag="ACZPCL_10875"
     CDS             290..751
                     /locus_tag="ACZPCL_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1163005.1"
                     /translation="MVDQQELSSAGLKFMDFAIRFFNMAKCQYQQQNQVKANKAGFQV
                     LFILSMPEYTQPTMSFLADEMGITKQQLTKLVNDLEAMGLVKRIHDERNRRQVYVTLT
                     DDGRNEFEEIKQAMLECTVAGLKDFSEDELEQLGDCLERLIPLLEKFRGNC"
     gene            complement(926..1675)
                     /locus_tag="ACZPCL_10880"
     CDS             complement(926..1675)
                     /locus_tag="ACZPCL_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1163006.1"
                     /translation="MSGNEKREPLIQVQNLGKSFGNIEVLKDITVDIYKGDVVFVVGP
                     SGSGKSTFLRCLNRLEEPTKGHIYFEGTDITDPKTDIDKHRQKMGMVFQQFNLFPHMD
                     IMKNLTLAPMKLQGKSQQEAEAEAMRLLERVGLADRAHAYPSQLSGGQKQRIAIVRAL
                     CMKPDVMLFDEPTSALDPEMVGEVLNVMRDLAAEKMTMVVVTHEMGFAREVATRVMFM
                     DGGYFMEEAAPEEFFSNPKNERLKSFLSKVL"
     gene            complement(1665..2357)
                     /locus_tag="ACZPCL_10885"
     CDS             complement(1665..2357)
                     /locus_tag="ACZPCL_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1163007.1"
                     /translation="MWKNFTDAFYLNFISDDRWHYIADGLKTTLIITFFACMMGIVLG
                     FIVGMIRSTHDKTGNLKLLNILCKIYLTVIRGTPVVVQLLIIYFVVFGSTRIDKVPVA
                     IMAFGINSGAYVAEIFRSGIMSIDPGQMEAGRSLGFNYAQTMWYVIMPQAFKTVLPTL
                     CNEFISLLKETSVSGYIAIQDLTKGGDIIRSRTYSAFMPLIAVALVYLVIVMIFTKLI
                     QILERRLRQSER"
     gene            complement(2405..3244)
                     /locus_tag="ACZPCL_10890"
     CDS             complement(2405..3244)
                     /locus_tag="ACZPCL_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1163008.1"
                     /translation="MKKRVIALTMAALMAASLTACGGSAKETTAAAAADTTAAAKEES
                     TAAESTAAESKEAAGGKLVMATNAEFPPYEFHDGDKIVGIDAEIAQAIADELGMELEI
                     EDIAFDSIIPEIVSGKADMALAGMTVTEDRKASVDFSDTYATASQMIIVKEDSEIAGP
                     DDLKGVTVGVQLGTTGDIYVSDLEADGTTVERYNKGFEAVQALSQGKIDAVVIDGEPA
                     KTFVSETEGLKILDEAFTVEEYAIAVKKGNTELLDKVNGALETLKDNGTLDEIVAKYI
                     KAE"
     gene            complement(3399..4235)
                     /locus_tag="ACZPCL_10895"
     CDS             complement(3399..4235)
                     /locus_tag="ACZPCL_10895"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HIR1163009.1"
                     /translation="MELIKGAPVSAKIKEEVGAMLEKINGPAPKLAIVRVGENPDDMS
                     YERGAVKKMDAFGLRSQCYTFPADITDEAFKKEFTAINEDPDVSGILLLRPLPKQICE
                     KDIEAMIDPKKDLDGISPVNIAKVFSGDSTGFAPCTAEAVIEVLKAYDISMEGKRAAI
                     VGRSMVVGRPLSMLLLKENATVTICHTRTKDLPGTCKQAEILVAAAGKAKMLDASYVG
                     ENTTVIDVGINVDENGKLCGDVAAETLEEKSGKLTPVPGGVGAVTTAVLAKHLVQAAL
                     RK"
     gene            complement(4257..4883)
                     /locus_tag="ACZPCL_10900"
     CDS             complement(4257..4883)
                     /locus_tag="ACZPCL_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HIR1163010.1"
                     /translation="MTEEKKISEYLEVLSSKAPVPGGGGASALAGALGNALGQMVVNL
                     TVGKKKYAEVEEEMQKYLTDLKNMQQEFLHFSDRDAEVFAPLAECYRLPSATLEEKEH
                     KDAVMEEKLLDASMVPVEIMEKSLELLEILDVLADKGSRMAVSDVGVAVQFTRTALLG
                     AVMNVYINTKSMKNREKAEEINQKAKRMIKIGTSQADEIYEKVLAQLI"
     gene            complement(4984..5760)
                     /locus_tag="ACZPCL_10905"
     CDS             complement(4984..5760)
                     /locus_tag="ACZPCL_10905"
                     /EC_number="6.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_function: GO:0061503 - tRNA threonylcarbamoyladenosine
                     dehydratase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HIR1163011.1"
                     /translation="MLNQFSRTELLFGKAAMEKLASSRVAVFGIGGVGGYTCEALVRS
                     GVGAFDLIDDDKVCLTNLNRQIIATRKTVGKYKAEVMKERMLEINPDVKVEIHKCFFL
                     PENADEFPFEDYDYIVDAVDTVTAKLELVMKAKEKGVPIISSMGAGNKLDPTMFRVAD
                     IYKTKVCPLAKVMRRELKKRGVKKLKVVYSEEQPTRPINDMAISCRNHCICPPGAAHK
                     CTERRDIPGSTAFVPSVVGLIIAGEVIKDLTKDVVVKAEE"
BASE COUNT         1414 a         1378 c         1251 g         1897 t
ORIGIN      
        1 tagtagtata aatagggttt tctgttattg ttattgctgc tgttgtcgtt ttttacgccc
       61 attgtatttt ctccttctat tttgttttat tttgattttg atcttctcgc gattacctgt
      121 gtttttcgct ttcttgatga taaaataaac ccgcttccag gttttgtcaa tagaaaatag
      181 taaattttta tttactttta ataattattg acatttcata tttgttgtgg taccgtttgt
      241 tatatggaac catataaaaa acacatcagg acaggaagga gactttttta tggttgatca
      301 gcaggagctt tcttctgcgg gcttaaagtt tatggatttt gccatccgtt tttttaatat
      361 ggccaaatgc cagtaccaac agcagaacca ggtgaaagcg aataaagcgg gatttcaggt
      421 cctttttatt ttaagtatgc ccgaatatac gcagcctact atgagttttc tggctgatga
      481 aatgggaatt acaaagcagc agctgacaaa gctggtgaat gatctggaag ccatggggct
      541 ggtgaagagg attcatgatg agaggaaccg gcggcaggtt tatgtgactt tgacggatga
      601 tggcaggaat gaatttgagg aaataaagca ggcgatgtta gagtgtacgg tggctgggct
      661 taaggacttt tcggaggatg agctggagca gctgggggat tgccttgaaa ggctgatccc
      721 gttgttggag aaattcaggg gaaactgcta atttaagctg ggctacagat atatataacc
      781 acctcaaaat tgcaaccaat ttaccttttt gagcatgtga ttttattttt ataatgtgcc
      841 ttgcacccac cgcgccgcca ggcgcggttc aatcatacat aaaaaaatga gtgtcaacaa
      901 agcttcatct gttgacactc attttttaca gcaccttact taagaaagac ttcaggcgct
      961 catttttagg atttgagaag aactcttccg gtgcggcttc ttccatgaag taaccgccgt
     1021 ccatgaacat gactctggta gcaacttctc ttgcgaagcc catttcgtgg gttaccacta
     1081 ccatggtcat tttttcagcg gccaggtcac gcattacgtt taatacctcg cctaccattt
     1141 ccgggtcgag agcggaagtc ggctcatcga agagcattac gtctggcttc atgcagaggg
     1201 ctcttacaat ggcgatacgc tgtttctgac caccggaaag ctggcttgga tatgcatggg
     1261 ctctgtcagc aaggccgaca cgctctaaaa ggcgcatagc ctctgcttct gcctcctgct
     1321 ggctcttgcc ctgaagcttc ataggcgcca gtgtcaggtt tttcatgata tccatatgcg
     1381 ggaacaggtt gaactgctgg aataccattc ccattttctg acggtgtttg tcaatatcgg
     1441 ttttcggatc tgtaatatcg gtaccttcaa aatagatatg gccttttgta ggctcttcca
     1501 gacggtttaa gcagcgtaag aaggtacttt taccggaacc ggatggtcct actacaaata
     1561 ctacatcacc tttgtagata tccactgtga tatcttttaa tacttcgatg tttccaaagc
     1621 tctttccaag gttctgcacc tggatcagag gctctctttt ttcattaccg ctcactctga
     1681 cgcagcctcc tttccagaat ctggatcagt tttgtgaaga tcataacaat aaccagataa
     1741 accagtgcaa ctgcgatcaa tggcataaac gcactgtagg ttctgcttcg gatgatatcg
     1801 ccgcctttgg taagatcctg gattgcgatg taaccggaaa cagaggtctc ttttaacaga
     1861 gaaatgaact cgttacacag agttggcagt acggttttga atgcctgagg catgatcacg
     1921 taccacatgg tctgagcgta gttaaagcca aggcttcgtc ctgcttccat ctgtcccgga
     1981 tcaattgaca tgataccgct tcggaaaatt tctgccacat aagcgccgga gttgatgccg
     2041 aatgccatga tcgctaccgg tactttatca atacgtgtgc ttccaaatac aacaaaatag
     2101 atgatcagca gctgaacaac tacaggtgta ccgcggataa ctgtcagata gatcttgcac
     2161 agaatattca gcagttttaa gtttcctgtt ttatcgtggg tagaacggat catacctacg
     2221 ataaatccca gaacaatgcc catcatgcag gcaaagaagg ttatgatcag tgtggtctta
     2281 agaccatctg caatataatg ccaccgatca tctgaaataa aattcagata aaatgcatct
     2341 gtaaaatttt tccacatgcc gatcatcctt ttctctctaa atacttgttt ctaatgacta
     2401 ttgattactc tgccttgatg tacttagcta cgatctcgtc cagagttccg ttgtccttta
     2461 aggtctctaa tgcgccgttt actttgtcaa gcagctcggt gttgcccttc tttacagcaa
     2521 ttgcatactc ttctacagta aatgcttcgt ctaagatctt taagccttct gtttcagata
     2581 cgaaagtctt tgctggctca ccatcgataa caactgcatc gatcttgccc tggcttaatg
     2641 cctgaacagc ttcaaagccc ttgttgtaac gctctacagt ggttccgtct gcttccagat
     2701 cagatacata aatgtcaccg gtagttccta actgaacacc aacagtaact ccctttaagt
     2761 catctggacc agcgatctcg ctgtcttcct taacgatgat catctgggaa gcagttgcat
     2821 aagtatcaga gaaatctaca gatgccttac ggtcttcagt tacagtcata ccagctaaag
     2881 ccatatcagc cttaccggat acgatctctg ggataatgga gtcaaatgcg atatcttcaa
     2941 tctctaattc cataccaagc tcatcagcaa tagcctgtgc gatctcagcg tcgataccta
     3001 cgatcttgtc tccgtcatgg aactcgtatg gtgggaactc agcgttggtt gccataacca
     3061 gtttgccgcc tgctgcttcc tttgactcag ctgctgtact ctctgctgca gtggattctt
     3121 cttttgctgc tgcagttgta tctgctgctg cagctgctgt tgtctctttt gcggaaccgc
     3181 cgcatgcggt taaggatgct gccataagag cagccattgt cagtgcgatt actctttttt
     3241 tcataatgtt ttctcctctt gcatgtttat gcatattatt atgtttgctc cctctatggt
     3301 gggaaagctg tctgttctat tataatgctt tttttctttt tagcaagagt aaatttatac
     3361 atagggttta tggggtttat gcaactctat atataatttt actttctgag agccgcctgt
     3421 accaggtgtt tggcaagaac ggcagtggta actgcgccta cgcctcccgg aacaggtgta
     3481 agttttccag atttttcctc taaagtttca gcagctacat caccgcaaag ttttccattt
     3541 tcatctacgt taatgcctac atcgatcaca gtagtatttt cacctacata ggaggcatct
     3601 aacatttttg cctttcctgc agctgctacc aggatctcag cctgtttgca ggtgcctgga
     3661 aggtctttgg tgcgggtgtg gcagatggtg actgtggcat tttcttttaa aaggagcatg
     3721 gacagaggac ggcctacaac catgctgcgg cctacgatcg ctgctctttt tccttccata
     3781 gaaatgtcat atgcctttaa tacttcgatc actgcctctg ctgtacatgg tgcaaatccg
     3841 gtagagtctc cggagaatac ttttgcaata tttaccggtg aaatgccatc caggtccttt
     3901 ttcgggtcga tcatggcttc aatgtctttt tcacagatct gctttggaag aggacgaagg
     3961 agaaggatac cgcttacatc cgggtcttcg ttgatagcgg taaattcttt tttgaatgct
     4021 tcgtctgtga tgtcagcagg aaatgtatag cactgggagc ggagtccgaa tgcatccatt
     4081 ttctttacag ctcctctttc gtaggacata tcatctggat tttctcctac gcggacgata
     4141 gccagctttg gtgccggtcc gtttattttt tcaagcattg cccctacttc ttctttgatc
     4201 tttgcggaaa caggtgcgcc tttgattaat tccatattaa ttttctcctt ttatgatcat
     4261 ataagctgtg ccaggacttt ttcgtagatc tcatctgcct gggaagtgcc gatcttgatc
     4321 atgcgttttg ccttctggtt gatctcttct gctttttcac gatttttcat ggatttcgtg
     4381 tttatatata cattcatgac agctccaaga agcgctgtac gggtaaactg taccgctacg
     4441 cccacatcgc tgactgccat gcggcttcct ttatctgcca ggacgtctaa aatttctaag
     4501 agttccaggg atttttccat gatctctact ggaaccatgc tggcatctaa cagcttttct
     4561 tccataacgg catctttgtg ctctttttct tccagggtgg cggatggcag gcggtaacat
     4621 tctgccaacg gcgcaaatac ttctgcgtca cggtcggaga agtgcaggaa ttcctgctgc
     4681 atgttcttta aatcagtcag gtatttctgc atctcctctt ctacttctgc gtattttttc
     4741 tttcctaccg tcagatttac taccatctgt cccagggcat ttcccagtgc tcctgctaaa
     4801 gcggatgctc cgccgcctcc cggaaccggt gccttagatg aaagaacctc caggtactca
     4861 cttatctttt tctcttctgt cattttttct cctcaataat ctctgtgtgc tgtctgcaca
     4921 gcagacatgt tgaaaccacc tgtgaaaaac acgggatgcg tattttgttt cctacgtttt
     4981 accttattcc tcagccttga caaccacgtc ttttgtcaga tccttgatca cttctcctgc
     5041 aatgatcagg cctaccacgg acggcacaaa tgcagtactt cccggaatat cccggcgttc
     5101 agtacattta tgagctgcac ctggcgggca gatgcagtgg tttctgcaac tgatggccat
     5161 atcgttgatc ggtcttgtag gctgctcctc ggagtaaacc acctttaatt tctttacgcc
     5221 tctttttttc agctcccggc gcataacttt tgccaacggg cagacttttg ttttgtaaat
     5281 gtcagctaca cggaacatgg taggatctaa tttatttccg gcgcccatgg agctgatgat
     5341 gggaactcct ttttcttttg ctttcattac cagttccagc ttggctgtaa cggtatctac
     5401 tgcgtctacg atatagtcgt aatcttcaaa tggaaattcg tctgcgtttt ccggcaggaa
     5461 gaagcatttg tggatctcta cctttacgtc cgggttgatc tccagcatac gttctttcat
     5521 cacttctgcc ttgtattttc ctacagtttt gcgggttgca atgatctggc ggtttaagtt
     5581 ggtcaggcat actttatcgt catcgatcag gtcgaatgcg cctactccgc ttcgcaccag
     5641 tgcttcacag gtataaccgc ctactccgcc gatgccaaat actgctacac gggaagaagc
     5701 cagcttttcc attgccgctt tgccaaataa taattctgtt cttgaaaact gatttaacat
     5761 gcatttttcc tctttatctg tcactcactt gtcagtagta tcatgctgtt ggtcatatag
     5821 tgcacaaaca gtgcacagac cataactatt acttaaaaaa tgatacttca tacccgctgt
     5881 cttgtcaatt ggcaaacctt tgggtataat agtcataact aactgcagat caaatccaaa
//
LOCUS       DBMDUA010000166         5879 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5406_length_5879_cov_2.961578, whole genome shotgun sequence.
ACCESSION   DBMDUA010000166 DBMDUA010000000
VERSION     DBMDUA010000166.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5406_length_5879_cov_2.961578"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="ACZPCL_10910"
     CDS             <1..1251
                     /locus_tag="ACZPCL_10910"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR1163012.1"
                     /translation="ALLTDQVISLGTGEQEVARNKEELRALMECEFKEMPQPLDYELF
                     NYTENPLGEYGWSIFTNVLAKIDSEGVTMEMLTRLTCICVKEDGKWKIASLHMSTPTV
                     EQEHQEFFPLRYGRNNSVRKLSSETGEKLMELVSEALPGGIMGSYLEEGYPLYTINDT
                     MLNILGYTYEELVDATNEKMMNIIYPPDQKWVEESIEKQFHEKNEYKVEYRIVGKGGR
                     IIWVSDIGKKIKSEDGRDAMISIMTDVSDRIERENQLIKEAQLDPLTGLYNRKRAISL
                     IEADFLKEKSGTLYICDVDNFKSMNDTKGHLAGDRALIHLAKLIRHYADENCVTARLG
                     GDEFMLYFTEDAEKKGADVIGKIQKEFAIAMKTSNPDLDITISAGGALRNAGEDFKDL
                     YKKADTALYLAKKEKGQLKIYKEQ"
     gene            complement(1268..2248)
                     /gene="bsh"
                     /locus_tag="ACZPCL_10915"
     CDS             complement(1268..2248)
                     /gene="bsh"
                     /locus_tag="ACZPCL_10915"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HIR1163013.1"
                     /translation="MCTAATYKTNDFYMGRTLDYEFSYGDEITVTPRNYSFTLRHGGK
                     LENHYAIIGMAHVAGNYPLYYDAINEKGLGIAGLNFVGNATYKPVDPSSSKHQITQFE
                     LIPFILGTCASLKEAKEALKNMDLVDTPFSPQLPVGSLHWLIADETGSITVEAVKEGL
                     MIYDNPAGVLTNNPPFPQQMFQLNNYMHLSPRQPVNTFSKDLPLNTYSRGMGALGLPG
                     DLSSSSRFVRASYVKMNSRCNGDELSSVSQFFHILGSVDQQRGCCEVAEGKYEITLYT
                     SCCNATKGIYYYTTYDNHQISAVDMHKTDLNGQELARFTPVTTEQIHFMN"
     gene            complement(2465..3634)
                     /locus_tag="ACZPCL_10920"
     CDS             complement(2465..3634)
                     /locus_tag="ACZPCL_10920"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HIR1163014.1"
                     /translation="MDINQASLQMHYDLHGKIEVISRKKIETREDLSLAYTPGVAEPC
                     RVIAKDYEQSFKLTRRSNLVAVITDGTAVLGLGDIGPAAGMPVMEGKCALFKEFADVD
                     AFPLCIDSKDTDTIVQTIYLISKSFGGINLEDIAAPRCFEIERRLKQMCDIPVFHDDQ
                     HGTAIVVAAALLNAVKVTKKEMGKLKIVINGAGAAGIAIGKHLINMGFGNVIMCDING
                     IICEGDEGLNPGQEEISHISNLNHEHGKLADALKGADAFIGVSRPNLVTKEMVSTMNN
                     GIVFAMANPTPEIMPDEALAGGAAVVGTGRSDFPNQINNVLVFPGVFKGALSVRAKEI
                     TELMKQRASYAIASMIPDEELTPENIIASPLNKKVADVVAKAVADTAIEEGIARV"
     gene            complement(3726..5090)
                     /gene="fumC"
                     /locus_tag="ACZPCL_10925"
     CDS             complement(3726..5090)
                     /gene="fumC"
                     /locus_tag="ACZPCL_10925"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR1163015.1"
                     /translation="MEYRIEHDSMGEVQVPADRYWGAQTQRSFQNFPIGTEKIPMEVI
                     RAFAILKKAAAIANNRLGKLDDKRLALISQACDEILAGKLDDNFPLAVWQTGSGTQSN
                     MNVNEVIAGRGNAIAGEKLLHPNDHSNMSQSSNDTFPTAMHIAAVMEIEDKLFPAIDR
                     LTDTFTRLMAENEGIVKTGRTHLQDATPISFSQEISGWREMLIKSKAMLTLSLKGLHE
                     LALGGTAVGTGLNAPKGFDTEVAKAVAELTGKDFVTAPNKFHSLTSKDELVFAHGALK
                     GLAADMMKMANDIRWLSSGPRCGLGEIFIPENEPGSSIMPGKVNPTQCESVTMVAVQV
                     IANDTAVGMAASQGNFELNVFMPVCIYNFLQSVRLLTDSLNSFNDRCVSGIRANREKM
                     DENLHRSLMLVTCLNPYIGYENAAKTAKKAYKENISLKEACVELGFLTAEKFDEIFHP
                     EQMV"
     gene            complement(5289..>5879)
                     /locus_tag="ACZPCL_10930"
     CDS             complement(5289..>5879)
                     /locus_tag="ACZPCL_10930"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HIR1163016.1"
                     /translation="GIDLGLKDFAIVSNGKTYKNINKSARLKKLEKQLIREQRRLSRK
                     YENLKKGEVTQRANIQKQKLKVQKLHHKIDNVRTDYINKTIAEIVKTKPSYITIEDLN
                     VKGMMKNRYLSKSVASQKFYEFRAKLPSKCNENGIELRVVARWYPSSKMCHRCGRIKR
                     DLKLSDRIYRCECGYVEDRDFNAALNLRDAITYEVA"
BASE COUNT         1592 a         1253 c         1351 g         1683 t
ORIGIN      
        1 gctcttctta cggaccaggt gataagcctt gggaccggag aacaggaagt ggcaagaaat
       61 aaagaggaac tgcgtgcttt aatggaatgt gaatttaaag aaatgcccca gcctcttgac
      121 tatgaactgt ttaactatac tgaaaaccct ctgggagagt atggatggag tattttcacc
      181 aatgttctgg caaagattga ctcagaaggt gtaacaatgg aaatgctgac ccgtctcacc
      241 tgtatctgtg tgaaagagga tggaaaatgg aagatcgcct ctttacatat gtctacccca
      301 actgtagagc aggaacatca ggaatttttc cctctccgtt atggcaggaa taacagtgtc
      361 cggaagcttt cttctgaaac cggcgaaaag ctgatggaac tggtgtcaga agcccttccc
      421 ggcggtatta tgggcagtta tctggaagaa ggatatcctc tttataccat taatgatacc
      481 atgttaaata tattaggata tacctatgaa gaactggtgg atgccacaaa tgaaaagatg
      541 atgaatatca tttatccacc ggatcagaaa tgggtggaag agagcatcga gaagcagttt
      601 catgagaaaa atgaatataa ggtggaatac cgtatagtcg ggaaaggcgg ccgtatcatc
      661 tgggtcagtg atattggaaa gaagataaag tcagaagacg gaagagatgc catgatcagt
      721 attatgaccg atgtctctga ccgtattgag cgggagaacc agctgatcaa agaagcacag
      781 ttagatcctc ttacaggact ttataaccgg aaaagggcca tcagcttaat agaggcagac
      841 tttttaaagg aaaaaagcgg gactctttat atctgtgacg tagataattt taagagcatg
      901 aatgacacaa aaggccacct ggcaggtgac agggctttga tccatctggc aaagctgatc
      961 cggcattatg cagatgagaa ctgtgtaaca gcacgactgg gcggtgatga gtttatgcta
     1021 tattttacgg aagatgcaga gaaaaaaggc gctgatgtta ttggaaagat acaaaaagag
     1081 tttgccattg ccatgaaaac gtccaatccg gatctggata ttaccatcag tgcaggcgga
     1141 gccttaagaa acgcagggga agattttaaa gatttatata aaaaagcaga cactgccctt
     1201 tatctggcaa agaaggaaaa agggcagctg aaaatttata aggagcagta gcagcctgct
     1261 tttaggatca gttcataaag tggatctgtt ctgtggtaac aggtgtaaaa cgggccagtt
     1321 cctgtccgtt caggtcggtc ttatgcatgt ctacagcgct gatctggtgg ttatcatagg
     1381 tggtataata ataaattccc tttgttgcat tgcagcagga ggtataaaga gtgatctcat
     1441 attttccctc agctacttcg cagcagcctc tctgctggtc cacagagcct aaaatatgga
     1501 aaaactggct gacactggaa agctcatccc cattgcaccg ggaattcatt tttacataag
     1561 aagcccggac aaaacgggag gaagaggaca gatctccggg aagtcctaaa gcgcccattc
     1621 cacggctgta ggtatttaaa ggcagatcct ttgaaaatgt atttacgggc tgtctgggtg
     1681 ataagtgcat atagttattt aactggaaca tctgttgcgg aaaaggagga ttgttggtca
     1741 ggacacctgc tgggttgtca tagatcatca gtccttcttt tactgcttct acggtaatag
     1801 agccggtctc atcggcaatg agccagtgga gacttcctac cggaagctgc gggctgaacg
     1861 gtgtgtctac caggtccatg ttttttaaag cttcttttgc ctcttttaaa gaagcacagg
     1921 tacccaggat aaaggggatc agttcaaact gggtgatctg atgtttggaa ctgcttggat
     1981 ctacgggttt gtatgttgcg tttccaacga aatttaagcc tgcgatgcca agaccttttt
     2041 cgttgatggc atcataataa agaggatagt ttcctgcaac atgggccatg ccaatgatag
     2101 catagtggtt ttccagcttt ccgccatgac gcaaagtaaa ggagtaattt cggggagtga
     2161 ctgtgatctc atccccataa gaaaattcat aatccagggt ccgtcccatg taaaaatcgt
     2221 tggttttgta agttgcggct gtacacatgt aagttgtcct ttctgttgtg ttgttttcgg
     2281 atcatcgtcc ggtgccatat ccgccaacgg cggatacggg gaataaaaca gtgcatttgc
     2341 ccgtcacagg cgggaaagat tgtagttata tatagcaaaa ggctgcagca gaatttgaaa
     2401 ttattctgcc acagccttta ttaaatatgt accggaccag cccatggctg aactgtaacg
     2461 tttattatac tcttgcgata ccctcttcaa tagcagtatc agcaactgcc tttgcaacta
     2521 catcagcaac cttcttgttt aatggggaag caatgatgtt ttccggagtc agttcctcat
     2581 ccgggatcat gctggcaatt gcgtaggaag ctctctgctt cattaattca gtgatttctt
     2641 ttgcacgaac agaaagagcg cccttgaata cacctgggaa taccaggacg ttgttgatct
     2701 ggttcgggaa gtcggaacga ccggtaccta caacagcagc gccgccagct aatgcttcat
     2761 ctggcatgat ctcaggagtc gggtttgcca tggcaaatac gataccgttg ttcatggtag
     2821 aaaccatttc ttttgtaacc aggtttggac gggatacacc gataaatgcg tcagcaccct
     2881 ttaaagcatc agccagcttg ccgtgctcat gatttaagtt gctgatatgg gagatctctt
     2941 cctggcctgg atttaagcct tcatcgccct cgcagatgat accgttgata tcacacatga
     3001 tcacattgcc aaagcccatg ttgatcaggt gtttgccgat tgcgatacca gcagctccag
     3061 caccgttgat gacgatcttt aatttgccca tttctttctt tgttactttt actgcgttta
     3121 ataatgcagc agcaactacg atagcagttc cgtgctggtc atcatggaat accggaatgt
     3181 cgcacatttg ttttaagcgg cgctcgatct caaagcatct tggagctgca atatcctcta
     3241 agttaatacc gccaaagctc ttggagatca gataaatggt ctgaacgatg gtatcggtat
     3301 ctttggagtc gatgcacagt gggaatgcgt ctacgtcagc aaattcttta aacagggcgc
     3361 attttccttc cataaccggc ataccggcag caggaccaat atctccaaga cctaaaactg
     3421 cagttccgtc tgtgataaca gctaccaggt tggaacggcg ggtcagctta aaggactgct
     3481 cataatcctt tgcgatcacg cggcaaggtt ctgcaacgcc tggtgtataa gcaagggaaa
     3541 gatcctctct ggtttcgatc tttttacggg aaattacctc gatctttccg tgaaggtcat
     3601 agtgcatctg aagggaagcc tgattgatat ccatagtgtg ttgtctcctt atgataatta
     3661 tttataccca tacgcccaac aggtggggcg tatattaaca atagctataa actgcaaagc
     3721 atcgcttata ccatctgctc cggatggaag atctcatcaa atttctcagc ggtcaaaaat
     3781 cccagctcta cgcaggcttc tttcagggag atgttctcct tataagcctt ctttgctgtt
     3841 ttcgctgcat tttcatatcc gatatatgga tttaagcagg taacaagcat cagggagcgg
     3901 tggaggtttt catccatctt ctcacggttt gcccggatgc cgctgacgca gcggtcatta
     3961 aaggaattta atgagtcagt aagcagacgt acagactgca ggaaattgta aatgcatacc
     4021 ggcatgaaca catttaactc aaaattaccc tgggaagctg ccatacctac agcggtgtca
     4081 ttggcaatga cctgaactgc taccatggta acagactcac actgagttgg atttactttt
     4141 ccgggcataa tagaactgcc aggttcattt tcagggatga agatctcacc aaggccgcag
     4201 cgtggaccgc tggacagcca gcggatgtca tttgccatct tcatcatatc ggcagccaat
     4261 ccctttaagg cgccgtgagc gaagaccagt tcatccttgc tggtgagaga gtggaatttg
     4321 ttaggtgctg taacgaagtc tttgccggtc agttcagcaa ctgcctttgc aacctctgta
     4381 tcaaagccct taggagcatt taaaccggta cctacagcag tgccgccaag tgccagttcg
     4441 tgaagtcctt ttaaacttaa agtaagcatt gccttggatt tgatgagcat ttctctccag
     4501 ccgctgatct cctgggagaa ggagatcgga gtagcatcct gcagatgggt acgtcctgtt
     4561 tttacaatgc cttcattttc agccatcagt cttgtaaaag tatcggttaa acggtcaatc
     4621 gccgggaaca gcttgtcctc gatctccata acggctgcaa tgtgcatggc agtcgggaaa
     4681 gtatcgttgg aactctggga catgttggaa tggtcatttg ggtgaaggag tttttcacct
     4741 gcaatggcat ttccacgtcc tgcaatgact tcgtttacat tcatattgga ctgggtgccg
     4801 cttcctgtct gccatacagc cagtggaaag ttgtcatcca gcttacctgc cagtatttca
     4861 tcgcaggcct gtgaaataag agcaaggcgc ttatcatcca gttttccaag gcggttattt
     4921 gcaatagctg ctgccttttt taaaatggca aaagcgcgga tcacttccat ggggatcttt
     4981 tcggttccaa tagggaagtt ctggaaactt ctctgggtct gggcccccca gtaacggtca
     5041 gccggaacct gtacttcgcc catagagtca tgttcaatac ggtattccat gaataatctt
     5101 atcctgccac tcaaaatgtg tgtaaatata cgcacatcga gataatatag gttctcacga
     5161 actctactta ctgcttcatc gtgtatgctt ttgattgacc gaaatcatat ctacttgcag
     5221 gttcttgtac actccacagt cgttaattcc cgaataagcc ttcggtacgt acttacgttt
     5281 gcgtttgact atgcaacttc gtaagttata gcatctctca aattaagtgc agcattaaag
     5341 tccctgtctt caacataacc gcattcgcat ctgtaaattc tatccgaaag ttttaaatct
     5401 ctcttgatgc gaccacagcg gtgacacatt tttgaagatg gataccatct ggctactact
     5461 cttaactcaa taccgttttc attacattta gatggaagtt tagctctaaa ctcataaaac
     5521 ttctgtgatg caacggattt tgaaagatat cggttcttca tcattccttt tacatttaag
     5581 tcctcaatcg ttatataaga tggtttggtt tttactatct cagcgattgt cttgttaatg
     5641 taatctgtac ggacattatc tatcttatga tgaagttttt gtaccttgag cttttgtttc
     5701 tgtatatttg ctctttgagt gacttctcct ttctttaagt tttcatactt tcgagagaga
     5761 cgcctttgtt ctcgaataag ttgcttttca agtttcttta atcttgcaga tttgttaatg
     5821 ttcttatacg ttttaccatt ggaaacaata gcgaaatcct ttaaaccaag gtcaatacc
//
LOCUS       DBMDUA010000167         5787 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5496_length_5787_cov_2.393598, whole genome shotgun sequence.
ACCESSION   DBMDUA010000167 DBMDUA010000000
VERSION     DBMDUA010000167.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5496_length_5787_cov_2.393598"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="ACZPCL_10935"
     CDS             complement(<1..567)
                     /locus_tag="ACZPCL_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HIR1163017.1"
                     /translation="MAFESLSDKLQNIFKNLRGKGRLSEADVKAALKEVKMALLEADV
                     SFKVVKQFISSIQERAVGEEVFGSLTPGQTVIKIVTEELVKLMGSETTEIALKPGNEI
                     TVIMMAGLQGAGKTTTTAKIAAKLKAKGKKPLLVACDVYRPAAIKQLQINGEKVDVPV
                     FSMGDKISPVDIAKAAVEHASKNGFNVVI"
     gene            complement(578..922)
                     /gene="ylxM"
                     /locus_tag="ACZPCL_10940"
     CDS             complement(578..922)
                     /gene="ylxM"
                     /locus_tag="ACZPCL_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HIR1163018.1"
                     /translation="MEKIVEQGLLYDFYGELLTEHQRRIYEDMVLNDLSPSEIAKEQG
                     ISRQSVHDLKKRCDKILEEYETKLQLVAKFMKIREMIRELDDLADTCAKTRDMALIDQ
                     IRKLSGEITATL"
     gene            complement(974..2467)
                     /locus_tag="ACZPCL_10945"
     CDS             complement(974..2467)
                     /locus_tag="ACZPCL_10945"
                     /inference="COORDINATES: protein motif:HMM:NF018837.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HIR1163019.1"
                     /translation="MGLFSNKKTQADGFSFAVDEVYALKEGKSVVVTGKMTEGKVTPG
                     TAAICLDSEGKPAFHCVIKGIEQGTQLMKMASADMKGTYGPHYGFKLDGAVKEDVPKG
                     AVLVPVTDELLEVVSETEEPAFKATPAPKVIDFNRISGLTMEDLQGVPEENENDLEAV
                     KKAGERDENLAALLPKKREDELSALVAGEGTLSEALLVPLSVKECIFMICRLQQMNEQ
                     AEMPDYNEKGQLLYDAVVEKLKMSSSLYILLDKGTGLPYIIEGTVDVYSEEILAKHAL
                     HFYENQYHKSLILKEVPKKNSGLPGRISLFAWLDYLGMEKLLINNGSYQLLMNRSDFL
                     EDPSEEKGAELPVPVSNPALRFEMADLLGEVRWPVSYPEREEKIAAKKNAVLNELVKS
                     KLLVPVKYNGDLNVGQNSLSAEQGQGLILPRITNKQKKAFLPLFTDWMEFEKAYKRND
                     WGCVVFSMADAIRATGGDNIVINPLAENLVLDREDIKEIINIYKNVK"
     gene            complement(2534..4114)
                     /locus_tag="ACZPCL_10950"
     CDS             complement(2534..4114)
                     /locus_tag="ACZPCL_10950"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HIR1163020.1"
                     /translation="MEQMKIQPEDWKEDYKEFDEATEKFYKGEMDAKTYKGISGGFGS
                     YAQRGGNASMLRLRMSGGVMDLAKLKFIADAIETYHIKRVHLTTCQTLQLHDLDEATV
                     KTLAVEALKCGIVTRGGGGDFPRNVTVSPLSGIEKGEYFNVLPWALAAADYLMTYIKG
                     PKLPRKLKVGFSNTPANLTHATFRDLGFAAREDGTFDVYSAGGLGNNPAFGVKVAEKV
                     EKDQILYYIEAMHQMFLAYGNYENRAKARSRYMQQTLGGAEKYKEAFLEKLKEVREMG
                     KDLTLTLPEAEVGCEAETGCDASTAVFTNSGRNRVYTQKQPGLYSVHCHPVGGTPDPS
                     LFVNLYKAICEIPGAELRLCPDESFYVINCREEDLEAVLHVTEDSAKTIFEESVACIG
                     AHVCQQGIRDSQGLLQKLVGMERNEQFADGILPKIHISGCPSSCGTHQIGVIGFRGGA
                     KTIDKVLKPAFNLYINGSDIQGQERMGEEVGTLLEEQIPDFLCALGHAVSDSGMDFDT
                     WFAKNPEGIKAIAASFLV"
     gene            complement(4129..>5787)
                     /locus_tag="ACZPCL_10955"
     CDS             complement(4129..>5787)
                     /locus_tag="ACZPCL_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HIR1163021.1"
                     /translation="GKVIKCNTIEELQANAIGITPDCLLYHTFRLQYSNPENYTRLAY
                     IGKDSMRKRVRLLEKELEEVSEKRNPLEETLKDCKRILALETLKEDTEEYFQWLKDID
                     TRKEQLKEKQKLSERLEKLRQKDVKQLEADRQAVLELSDGKKRERDLLQEQILNKKNE
                     LERFSQTILKLETEAAGQGKEVPVNEEHERELNAYLADKDHPRFDQEKEAYASKCQIL
                     EQKAVQAKEHVMGLRSEYIRRYPGRNVPVSSNDNAAYDKILNRLSCDDLEVYREQAGK
                     QAKAAVEHFKDDFMYKIRSAIKDALLRKDELNQIISRLDFGKDKYQFVIGRCKGADGR
                     FYDMFMDESLEINPASLKTGVENQMDLFSMEHENRYGDLINELISIFIPPENATAAQL
                     EEARKNMEKYADYRTYLSFDMQQLIKNEDEVIRIGLSRMIRKNSGGEGQNPLYVALLA
                     SFAQAYHINLSSAVRQRPSIRLVILDEAFSKMDAEKVASCIELIRGLGFQAIISATND
                     KIQNYVENVDKTFVFANPSKKSISIQAFEKDRMKDLASEMDEEA"
BASE COUNT         1405 a         1486 c         1120 g         1776 t
ORIGIN      
        1 gatcactaca ttgaagccat ttttagaagc atgttctaca gcggcttttg caatgtccac
       61 cgggctgatc ttatcgccca tggaaaatac aggaacatct accttttcac cattgatctg
      121 cagctgtttg atcgcagccg gacgatatac gtcacaggca actaaaagag gcttctttcc
      181 ttttgctttt aacttagcag cgatctttgc tgtagttgtg gttttaccgg cgccctgaag
      241 acctgccatc ataatgactg tgatctcatt tcccggtttt aaggctattt ctgtggtttc
      301 agagcccatc agctttacca gttcttcggt tacgatcttg ataaccgtct gtcctggtgt
      361 caggcttccg aatacctctt cccctacggc tctttcctga atggaactga tgaactgctt
      421 cactactttg aagcttacat ctgcttccag gagtgccatt ttaacttcct ttaaggcagc
      481 ttttacatct gcttcagaaa gtcggccttt gccccgcagg tttttgaaaa tattctgcaa
      541 tttgtcggat aagctttcaa aagccattca taacctccta aagtgtggct gtaatctcac
      601 cggataattt ccggatctgg tcgatcagag ccatatctct ggtttttgca caggtatctg
      661 ccagatcatc taactcccgg atcatctccc ggatcttcat gaacttggct accagctgca
      721 gttttgtttc atattcttcc agtatcttgt cacaccgctt tttcagatcg tgaacgctct
      781 gacggctgat cccctgctcc tttgcgatct cgctgggaga aagatcattt aacaccatgt
      841 cttcatagat acggcgctga tgttccgtca gcagttcacc atagaaatcg tacaacagac
      901 cctgttcaac gatcttttcc atttcaacca cctgtgctat catacacgat ggcagaaaga
      961 atgtcaagta tttttacttg acgtttttat aaatattaat gatctctttt atatcttctc
     1021 tgtcaaggac cagattttca gccagagggt tgataacgat gttatcacca ccggttgcgc
     1081 ggatggcatc agccatggaa aatacaacac agccccagtc attgcgctta taagcctttt
     1141 caaattccat ccagtcagta aacagcggaa ggaaggcttt tttctgcttg tttgtaattc
     1201 ttggaaggat cagtccctgt ccctgctctg cacttaagct gttctgtcca acatttaagt
     1261 caccgttgta tttaacagga accagtaatt tgctctttac cagttcattt aatacagcgt
     1321 tcttctttgc agcgatcttc tcctcccgtt ccggataaga tacaggccat cttacttctc
     1381 ctaacaggtc tgccatttca aagcgcagag ccggattgga tacaggaacc ggaagctctg
     1441 ctcctttttc ctcagatgga tcctctaaga agtcagaacg gttcatcaac agctgatagg
     1501 agccgttatt aataagaagt ttttccattc caaggtagtc aagccatgca aacaggctga
     1561 tccgtcctgg aagtccggaa ttcttctttg gaacttcttt taagatcagg gacttgtggt
     1621 actggttctc atagaaatga agggcatgct ttgccaggat ctcctcagag taaacatcaa
     1681 cggttccttc aatgatataa ggaagtccgg tacccttatc taagagaata taaagagaag
     1741 aagacatctt taacttctca acaaccgcat cataaagcag ctgtcctttt tcattgtaat
     1801 ctggcatttc tgcctgctca ttcatctgct gtaaacggca gatcatgaag atgcattctt
     1861 ttacagaaag cggaaccaat aatgcctcgg aaagagttcc ttctcctgca accagggcag
     1921 aaagttcatc ctctctcttt tttggcagaa gggcagccag gttttcatct ctctctcctg
     1981 ctttttttac agcctccagg tcattttcat tttcctctgg tacgccctga agatcctcca
     2041 tggtaaggcc ggagatcctg ttaaaatcga tcacctttgg tgcaggtgtt gccttaaaag
     2101 caggctcttc tgtctcactg acaacctcta acagctcatc tgtaacaggt actaacacag
     2161 ctcccttcgg aacgtcctct tttacagcac cgtctaattt aaagccgtaa tgagggccat
     2221 aggttccctt catatcagca gaagccattt tcattaactg ggttccctgt tcgattccct
     2281 tgatcacgca gtggaaagca ggctttcctt cgctgtccaa gcagatggct gcagtaccgg
     2341 gagtcacctt tccttctgtc atcttacctg tgacaactac gctttttcct tcttttaatg
     2401 cataaacttc gtctacagca aatgaaaatc catctgcctg tgttttttta tttgaaaata
     2461 atcccattgt ttcctcctaa aatcgggcat gatatgaaga ttatacaaca gcccggctta
     2521 aatgaaaagc cttttatacc aggaaagaag ctgcaatggc cttgatgcct tccggatttt
     2581 ttgcaaacca tgtgtcaaaa tccattccgc tgtcgcttac agcatgtcct aaggcacata
     2641 agaaatccgg gatctgctct tccaataaag ttcccacttc ttctcccatg cgctcctggc
     2701 cctggatatc gctgccgttg atataaaggt taaaagcagg ttttaatact ttatcaatgg
     2761 tctttgcacc gcctctgaag ccgatcacac cgatctggtg ggtaccgcag gaagacgggc
     2821 agccggaaat atggatcttt ggcaggatac cgtcagcaaa ctgctcgttt ctttccattc
     2881 ctactaattt ctgtaaaagt ccctgggagt cacggatacc ctgctggcat acatgggcac
     2941 cgatacaggc tactgactcc tcaaaaattg tcttcgcact gtcttctgtt acatgtaaaa
     3001 cagcttctaa gtcttcttcc cggcagttga tcacatagaa ggactcatcc ggacagagac
     3061 gaagttctgc acctgggatc tcacagatag ccttgtataa gtttacaaat aaagatggat
     3121 ctggtgttcc gcctaccgga tggcagtgta cactgtaaag ccctggctgt ttctgggtat
     3181 atacccggtt tcttccagaa tttgtaaata cagccgttga agcatcacag ccagtctctg
     3241 cctcacagcc cacttcagcc tcaggaagcg ttaaagtcag gtcctttccc atttcccgca
     3301 cttcttttaa cttctccaaa aatgcctctt tatatttttc agcaccgcct aacgtctgct
     3361 gcatataccg gcttcttgcc tttgcacggt tctcataatt gccgtaggct aaaaacatct
     3421 ggtgcatagc ttcaatgtaa taaaggatct ggtccttttc cactttttca gctaccttta
     3481 caccaaaagc aggattattt ccaagaccac ctgcactgta aacatcaaaa gtaccgtctt
     3541 ctcttgcagc aaagcccaga tcacggaaag ttgcgtgggt caggttagca ggcgtattag
     3601 aaaaacctac ttttaacttt cttggaagct tcggtccttt aatataagtc atcaggtaat
     3661 ctgctgcagc cagagcccat ggaagcacat taaaatattc ccctttttca atgcctgaca
     3721 acggagaaac cgtcacattt ctcgggaaat ctccgccacc gcctctggta acaatgccgc
     3781 attttaaagc ttccacagca agagtcttta cagtcgcctc atccagatca tgaagctgta
     3841 aggtctggca ggttgttaaa tgtacccgtt tgatatgata ggtttcaatg gcatctgcaa
     3901 taaacttaag ctttgccagg tccattacac cgccggacat gcgaagacgc agcatacttg
     3961 catttccgcc acgctgcgca tagcttccaa aaccgccgga aatccccttg taggttttgg
     4021 catccatttc ccctttgtaa aatttttctg tagcctcgtc aaattctttg tagtcctctt
     4081 tccagtcttc cggctggatc ttcatctgtt ccataagtat ctctcctttt atgcctcttc
     4141 atccatttct gacgccagat ctttcatgcg gtccttttca aacgcctgga tggaaataga
     4201 ttttttactt ggatttgcaa atacaaaggt tttatctaca ttctccacat aattctggat
     4261 cttgtcattg gtagcactga taatggcctg aaagcccagt ccacggatca gttcaataca
     4321 gcttgccacc ttttccgcat ccatcttgga aaatgcctcg tctaaaataa ccaggcggat
     4381 ggacggcctc tgccgtacag ctgatgacag gttaatatga taggcctgtg caaaacttgc
     4441 aaggagcgcc acatataatg ggttctgtcc ctcaccgccg gaatttttgc ggatcatacg
     4501 gcttaagccg atccggatca cctcatcctc atttttgatc agctgctgca tgtcaaaaga
     4561 aagataggtg cggtagtctg catatttttc catatttttc cgtgcttctt ctaactgggc
     4621 tgctgtagca ttttccggcg ggatgaaaat gctgatcagt tcgttgatca gatccccata
     4681 ccggttttca tgttccatgg aaaaaaggtc catctggttt tccaccccgg tctttaaact
     4741 ggcgggattg atctccaatg actcatccat gaacatatca taaaaccgtc cgtcagcccc
     4801 tttgcaccgg ccgatcacaa actggtattt atcttttcca aagtccagac ggctgatgat
     4861 ctggtttaac tcatccttgc gcaaaagagc gtccttgata gcactgcgga tcttgtacat
     4921 aaaatcgtcc ttaaaatgct ccacagccgc ttttgcctgc tttccagcct gttccctgta
     4981 aacttccagg tcatcacagg aaagacggtt taagatcttg tcatacgctg cattgtcatt
     5041 gctgcttaca ggaacattcc ttcccggata ccggcggatg tattcacttc taaggcccat
     5101 tacatgttcc ttcgcctgaa ctgccttctg ctctaagatc tggcacttgg acgcataagc
     5161 ctctttttcc tggtcaaagc gtggatgatc cttatctgcc agataagcat tcagttcccg
     5221 ttcatgttcc tcgtttacag gtacttcctt cccctgacct gcagcctctg tttccagctt
     5281 taagatagtc tgggagaaac gttccagttc atttttcttg tttaggatct gctcctgtaa
     5341 cagatctctc tcccgtttct ttccgtcact gagctctaaa acagcctgac ggtcggcttc
     5401 taactgcttt acatccttct ggcgaagctt ttccagccgt tcagacagtt tttgcttttc
     5461 ctttaactgt tctttgcggg tgtctatatc ctttaaccac tggaaatact cctctgtatc
     5521 ctcttttaag gtttccaaag caaggatccg cttgcagtcc tttaacgttt cctctaacgg
     5581 atttctcttt tctgaaacct cctccagttc cttttcaaga agacggaccc tcttgcgcat
     5641 actgtctttt ccaatatagg caagacgggt ataattttcc ggattgctat actggagacg
     5701 gaaagtatga tataaaagac agtccggggt aataccaatg gcattggcct gtaattcctc
     5761 aatggtatta catttgatca ccttgcc
//
LOCUS       DBMDUA010000168         5777 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5509_length_5777_cov_3.392001, whole genome shotgun sequence.
ACCESSION   DBMDUA010000168 DBMDUA010000000
VERSION     DBMDUA010000168.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5509_length_5777_cov_3.392001"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(96..674)
                     /locus_tag="ACZPCL_10960"
                     /pseudo
     CDS             complement(96..674)
                     /locus_tag="ACZPCL_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577793.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            817..1356
                     /locus_tag="ACZPCL_10965"
     CDS             817..1356
                     /locus_tag="ACZPCL_10965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1163022.1"
                     /translation="MEFNQTVMARRSLRAYEEGKTVEKAQIEELIRFAQMAPSWKNSQ
                     TGRYYVVMSPEMLEKVRKDCLPEFNQKNSANAPALIITAFEKTRSGFTREGVAENEVG
                     EGWGCYDLGLQNENLVLKAKDMGLDTLIMGIRNSEKLREYLEIPASQEVVSVIAVGYG
                     AIKPEMPVRKNVEDIVKFF"
     gene            complement(1368..1808)
                     /locus_tag="ACZPCL_10970"
     CDS             complement(1368..1808)
                     /locus_tag="ACZPCL_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR1163023.1"
                     /translation="MTTIRPMQPADKEAVLAMMEVFYASPAVLSNGSAAIFSSDFEAC
                     INDNPYLEGYMFEENGQIQGYAMAAKSFSTEFGRPCIWVEDLYIKEDFRGTGIGSLFL
                     EFIEKKYPEAVLRLEVEEENGRAVKVYEKNGFNVLPYMEMKKLL"
     gene            complement(1813..2967)
                     /gene="nagA"
                     /locus_tag="ACZPCL_10975"
     CDS             complement(1813..2967)
                     /gene="nagA"
                     /locus_tag="ACZPCL_10975"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR1163024.1"
                     /translation="MIIDNVKVYTPDQKFVEGGLVLDQDRIEAVYTNETKPDLSGKEV
                     LDGEGAYAIPGLIDLHFHGCKGDDFCDNSMEAIERIAEYEASVGVTAIAPATMTLPVE
                     ELVDVLKTAAQYKREKHDYKKADLVGINMEGPFISPVKKGAQDDRNILPCDIALCERF
                     LEASEDLVKFVGIAPEESENSLEFIRHMKEKVDISLAHTNADYDTAMAAFSAGANHAV
                     HLYNGMPEILHRAPGVVGAVFDSKHVMTEIICDGIHIHPASVRATFQMMGADRMILIS
                     DSMRATGMPDGQYTLGGLDVKVVGKLATLVSDGAIAGSATNLADCMRTVVQKMDLPLE
                     TAVACATINPARSLKIDKDYGSLEKGKKANVVLLGKDLELKCVIKDGKRI"
     gene            complement(3095..3415)
                     /locus_tag="ACZPCL_10980"
     CDS             complement(3095..3415)
                     /locus_tag="ACZPCL_10980"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1163025.1"
                     /translation="MEAFAVESYIQTYPGQDVDNARIFSRCHITPNTQFSTMDINATY
                     SMVEAGLGIGINNQVNSSLWQDRVVHLPLKPAQTMNIGLAYGKNISPASEGFLHFIED
                     KLPE"
     gene            3414..3791
                     /locus_tag="ACZPCL_10985"
     CDS             3414..3791
                     /locus_tag="ACZPCL_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HIR1163026.1"
                     /translation="MGTDFSLANGCPGGIRATMGFMIGNKTDGAFLAATGYLKIVAVF
                     YVFCFTGNTFAGYFDGIGRVNIPFIGAVGHITLRVILSWIFVSQFQLNAVAVATGIGW
                     MLVNLFWSGCYFSSTTWKIINFK"
     gene            complement(3749..4981)
                     /locus_tag="ACZPCL_10990"
     CDS             complement(3749..4981)
                     /locus_tag="ACZPCL_10990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR1163027.1"
                     /translation="MNYDDLFRETACVRIKKGTGRTLKAGGAWIYDNEIDTISGDFEN
                     GDLVTVEDFDGYPLGHGFINTSSKLTVRMLSRKKDTVVNEDFIEQRVRTAWEYRKATT
                     DISSCRVIFGEADFLPGIVIDKFEDVLVVESLALGIDRLKPVILDKLKKVLAEDGILI
                     RGIYERSDAKVRLQEGMERFKGFIGEPFDTKVEICENGVHYIVDVEDGQKTGFFLDQK
                     YNRLAVQNLCRNLKPAKVLDCFTHTGSFALNAGIAGSEKVLGVDASQLAVDQATENAR
                     LNGLEDHVTFQCADVFDLLPKLEQEGEKFDVVILDPPAFTKSRNSIKNAVKGYREINL
                     RGMKLVKDGGYLATCSCSHFMDPELFTKTIREAASNVHKRLRQVEYRTQAADHPILWA
                     ADESYYLKFIIFQVVDEK"
     gene            complement(5088..5699)
                     /locus_tag="ACZPCL_10995"
     CDS             complement(5088..5699)
                     /locus_tag="ACZPCL_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1163028.1"
                     /translation="MSTEAVDRRVRRTRKQLRECLVTLLKQKKVQDITVRELTELADL
                     NRGTFYLHYKDVFDLLEKTESELLDDFNQLVMKHDAEDLKKHPYGIFVEIYTLAYDNA
                     DLVEILLGENGDLNFLNRLKQLIRDKCLHDWMEVFRAGNPAIFDAYFSFIVSGCVGLV
                     QYWLKTGMKETPQQLARLTEQVIRKGIGVLDTDPFADIENVRS"
BASE COUNT         1538 a         1260 c         1296 g         1683 t
ORIGIN      
        1 agtgataggc agtgagatga gtggaggcgt gcgtaaggtg actatttcca attgcatttt
       61 cgatggaacg gaccggggca ttcgtctcaa gtccacccgg taacctgtgt atataggttt
      121 tccaaatcct ttaattcttt ttcaaaactc tgtttgtcag agatctttga catatccggg
      181 tgatggtagg tatgattgcc aacgatatgg ccttcttcta ccatccgttt taccagttcc
      241 ggactggttt ccagataatt tcccactaca aaaaaggcag cgtgaacgcc atgtttcttt
      301 aaggcatcta atatctttgc ggtattccca ttttcataac cagcatcaaa ggtgagatag
      361 attacttttt caccaggatc tcccatatag caggcattaa actgctttaa gtagtcattt
      421 gttgtatttc caacgggagc ctgaccggga gagggaaagc ttaagcccca gttgccgtca
      481 gcagaggcag aataaatggg gataccattt tcagttaaag ccactggctt ttgggaaggt
      541 tcataaacgg cattttttgt agactcttca gcagaagaaa actgatttgc agcctgcccc
      601 gccagacttc ctgccagata ggcaaaaaca aagatcaaaa ccagatggaa ccattttttt
      661 acaaaagcac ccatagaaaa actccagaca aaagggattg gtgtatgtat attccaaagt
      721 ggctgcggat atgttatact tctgattaga aagcaaaaag gtaactgtga aagcaatgaa
      781 tagttacgca aaaagttatc aaggaggcta attactatgg aatttaatca gaccgttatg
      841 gcgcgcagaa gcttaagagc ttatgaagag ggaaagacag tagaaaaggc acagatcgag
      901 gaactgatcc gttttgccca gatggctccg tcctggaaaa attcccagac cggcagatat
      961 tatgttgtaa tgtcaccgga aatgctggaa aaagtaagaa aagactgcct tccggaattt
     1021 aaccagaaaa acagcgcaaa tgctcctgca ctgatcatca ctgcttttga aaaaacacgt
     1081 tccggtttta caagagaagg tgtggctgaa aatgaagttg gcgaaggctg gggctgctat
     1141 gacctgggac tgcagaatga aaacctggtg ttaaaggcaa aagatatggg cttagatact
     1201 ctgatcatgg gaattcgcaa cagcgaaaag cttcgtgaat acttagaaat tccggcatcc
     1261 caggaagttg tttccgtgat cgcagtagga tacggagcaa ttaaacctga aatgccggta
     1321 agaaagaatg tagaagatat tgtaaaattc ttctaggtga ccgctggtta taacagtttt
     1381 ttcatttcca tatacggcag gacgttaaag ccgtttttct catagacttt aacggctctg
     1441 ccgttttctt cttctacttc caggcgaagg acggcttcag gatacttttt ttcaataaat
     1501 tccaaaaata agcttccaat gccggttcca cgaaaatcct ctttgatata aaggtcttcc
     1561 acccagatac agggacggcc gaattctgtg gaaaagcttt ttgcagccat ggcatatccc
     1621 tggatctggc cattttcctc aaacatatag ccttccagat aaggattatc gtttatgcag
     1681 gcttcaaaat cactggaaaa aatagcagcg gagccgttgc ttaaaacagc cggggaagcg
     1741 tagaaaactt ccatcattgc aagaacagcc tccttgtcag caggctgcat aggtcttatg
     1801 gttgtcattt tttcagatcc tctttccgtc tttgatcaca cattttaatt caagatcttt
     1861 tccaagaagt actacattgg cttttttacc cttttcaagg gagccgtagt ctttatcgat
     1921 ctttaagctt ctggctggat tgatggtagc gcaggcaacg gctgtttcta atggcaggtc
     1981 cattttctga acaacggtac gcatacaatc agccagattg gtggcagaac cggcaatagc
     2041 tccgtcagaa actaaagttg ccagttttcc tactactttt acatccagac cgcctaaggt
     2101 atactggccg tccggcatac cggtagctct catactgtca ctgatcagga tcatgcggtc
     2161 agcgcccatc atctggaagg tagcgcgtac agaagccggg tggatatgga tgccatcaca
     2221 gatgatctca gtcattacat gtttgctgtc aaatacagca cctacaacgc cgggagcacg
     2281 gtgcaggatt tccggcatac cgttgtataa gtggacagcg tggtttgccc ctgcgctaaa
     2341 tgcagccatg gctgtatcgt aatcggcgtt ggtatgagcc agtgagatat ctactttctc
     2401 tttcatatgg cggataaatt ccagtgagtt ttcgctttct tccggtgcaa tgcccacaaa
     2461 ttttaccaga tcttcagaag cttctaagaa gcgttcgcaa agtgcaatat cacagggaag
     2521 gatattccgg tcatcctgtg cgcctttttt tacagggctg ataaaggggc cttccatgtt
     2581 gatgcctacc aggtctgcct ttttatagtc atgtttttca cgtttgtact gtgcggcagt
     2641 ttttaaaaca tctaccagtt cctctaccgg aagtgtcatg gtagcaggtg caatagcagt
     2701 tactcccaca gaagcttcat attctgcgat ccgttcaata gcttccatgg aattgtcaca
     2761 gaaatcatcc cccttgcatc cgtgaaaatg caggtcgatc agtcctggaa tggcataggc
     2821 gccttctcca tcgagaacct cttttcccga aagatccggt ttggtttcat tggtgtagac
     2881 agcttcgatc cggtcctgat ccagtaccag accgccctct acaaatttct gatcgggtgt
     2941 ataaactttt acattatcaa tgatcatgtt gtaatccctc cagtttttta tttatgatga
     3001 catatttgtt ttgtgttccc ctgttactta gtgtacttgg aaatgagttt ttttgcaata
     3061 tgggggtaaa aatggccggt tgctgcagga atgtttattc cggcagctta tcttctataa
     3121 agtgaaggaa gccttccgaa gccggggaaa tattctttcc ataggccagc ccaatattca
     3181 tggtctgggc cggttttaag ggaagatgca cgacccggtc ctgccagaga gaactgttga
     3241 cctgattgtt gatgccaatg ccaagccctg cttctaccat agaataggtt gcattaatgt
     3301 ccatagtgga aaactgggtg ttaggagtaa tgtggcagcg ggagaaaatg cgggcattat
     3361 ccacgtcctg tcccggataa gtctggatgt aggactctac agcaaaggct tccatgggaa
     3421 cggatttttc tttggctaat gggtgtcccg gtggaataag ggctaccatg ggatttatga
     3481 tcgggaataa aacagatggt gcctttctgg cagccacagg atatttaaag atcgttgcgg
     3541 tattctatgt gttctgcttt acaggaaaca catttgcagg ctattttgac gggatcggca
     3601 gagtaaatat cccctttata ggagctgtgg gacacattac tctgcgggtg atcctgtcat
     3661 ggatatttgt aagtcagttc cagttaaatg cagtagccgt agctaccggc attggctgga
     3721 tgttggtaaa tctgttctgg agcggatgtt atttttcatc cacaacctgg aagataataa
     3781 attttaaata ataggactca tctgccgccc aaaggatggg atgatcggct gcctgggtgc
     3841 ggtattctac ctggcgcagt ctcttgtgga cattggaagc tgcttcacgg atggtcttgg
     3901 taaacagctc cggatccatg aagtgggagc aggagcaggt ggcaaggtag ccgccgtctt
     3961 ttactaattt catgccacga aggttgatct cacggtagcc ttttactgcg tttttaatgg
     4021 agtttctgga tttggtgaaa gctggcgggt caaggatgac tacatcaaac ttttcacctt
     4081 cctgctctaa ttttggaagc aggtcaaata catcggcaca ctggaaagtg acgtgatcct
     4141 ccaagccatt taagcgggca ttttctgttg cctggtctac tgccagctgg gaagcgtcta
     4201 cgccaaggac tttttcagag cctgcaatac cagcatttaa agcgaaagag ccggtatggg
     4261 tgaagcagtc taataccttt gcaggtttca gattacggca caggttctgt actgcaagac
     4321 ggttgtattt ctgatccagg aaaaatccgg tcttctggcc gtcttctacg tctacaatgt
     4381 agtgaacgcc gttttcacag atttctacct tggtatcgaa tggttctcca ataaagcctt
     4441 taaagcgctc cataccttcc tgaaggcgga cttttgcatc gcttcgctca taaatgccac
     4501 ggattaaaat accgtcttct gccaggactt tctttaattt atctaagatc accggcttta
     4561 agcggtcaat gcccagtgcc agggactcta ctactaaaac atcttcaaat ttatcgatga
     4621 caataccagg cagaaaatct gcttcgccga agatcacgcg gcagctggag atatcagtag
     4681 tagcttttct gtattcccag gcagtgcgga ctctctgttc gatgaaatct tcattgacca
     4741 cagtatcttt tttgcgggat aacatgcgta cggtcagctt ggagcttgta ttgataaagc
     4801 catgtcccaa aggatagccg tcaaaatctt ccacagtgac caggtcgcca ttttcaaaat
     4861 caccggaaat agtgtcgatc tcattgtcat agatccaggc gccgccggcc tttaatgtgc
     4921 gtccggttcc tttttttata cgtacacagg cagtttctct gaataaatcg tcgtagttca
     4981 tctgttttct cctcatttgg tgtaatatac tctcagaata tcatcagtat acaacaaaat
     5041 ataagcaatc gtatattatc aatatattga taataatata gactggatca ggaacgtaca
     5101 ttttctatat ccgcaaaagg atctgtatcc agcacaccga tgcctttacg gatcacctgc
     5161 tcggtcagtc gtgccagttg ctgcggtgtc tctttcatgc ctgtctttaa ccagtactgc
     5221 accagtccta cacagccgga tacgatgaaa gagaagtagg catcaaaaat agctggattt
     5281 ccggcgcgga atacttccat ccagtcgtga agacatttat cacgaatcag ctgttttaag
     5341 cggttcagaa agtttaaatc accgttttca cctaataata tttctaccag gtctgcattg
     5401 tcataggcca gtgtgtaaat ttctacgaaa ataccgtaag gatgcttctt cagatcttct
     5461 gcatcatgtt tcatgaccag ttgattaaaa tcgtctaaaa gctcgctttc tgttttttcc
     5521 agaagatcaa atacatcttt ataatgaaga taaaaagttc ctctgttcag atcggccagc
     5581 tcggtcagct cccggacagt aatatcctgc acttttttct gttttaataa agtaacaaga
     5641 cattcccgca gctgctttct ggtacgccgt acccgtcgat ctactgcttc tgtactcatg
     5701 ataagaaatt cccccttttt gtatgtgatg ttctctcgga tttgtttaca gtattttaat
     5761 attttgttta tgaacat
//
LOCUS       DBMDUA010000169         5760 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5527_length_5760_cov_4.373918, whole genome shotgun sequence.
ACCESSION   DBMDUA010000169 DBMDUA010000000
VERSION     DBMDUA010000169.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5527_length_5760_cov_4.373918"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            159..848
                     /locus_tag="ACZPCL_11000"
     CDS             159..848
                     /locus_tag="ACZPCL_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HIR1163029.1"
                     /translation="MKHLQRFAGILTAFCLMIILFFTSVEAVVYWTPGYFEKEYTKYQ
                     VLDDLPSMTMDDLLDVTDQMMAYLKGKRADLHVTTHMGGETREFFNEREIAHMEDVQG
                     LFLKAIALRRICLVVCVVMLLFMILSKADLRTLLPSSLCIGTGLFFAVIAALAAIIST
                     NFTKYFIMFHHMFFTNDLWILDPTTDMLINIVPEGFFMDTAGRIAFLFGALSLILFGI
                     CLIMTLKNKRR"
     gene            864..2393
                     /locus_tag="ACZPCL_11005"
     CDS             864..2393
                     /locus_tag="ACZPCL_11005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1163030.1"
                     /translation="MKRILSFILSINLIFILVPGTVLAAPSWPSAVSIEADGGILMEA
                     QTGTVLFAKNEDQPYYPASITKILTALIVLEHCSLDEEVTFSHDDVYNVEAGSSTAGI
                     DEGDVLTVRDCLYALMLASANESANALACHVSGSREAFAELMNQTAESLGCTGSHFAN
                     PSGLNDENHYTTAHDMALITRAAIQNPDFLEIDGARNYKLPPTKRNLEGGYVANHHRM
                     LVKNTSVYYPGAFAGKTGYTSIAGNTLVTCAEKNDMTLIAVVLNGHQTHYSDTKALFD
                     FGFDRFQALKAVDHETSYQALDNDMTIGGMTAQDNISLELDKNSLVIIPKDADFSDTE
                     ASLSYELDENAPDHAIARILYEYQGHSIGQVYLCSAAHTQANTENISAGSQALAAQTE
                     TGTAAPAETPEQKEIPDANARSSAQTRSSASKKAFPFLLVGICSATLIIVGVILFLLL
                     HSHRKEKEDLLLRRKRRLERLEDIGYSSSDFDQLLSQKRSSTPAYKKQTHKVKRPRKK
                     W"
     gene            2464..3585
                     /locus_tag="ACZPCL_11010"
     CDS             2464..3585
                     /locus_tag="ACZPCL_11010"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1163031.1"
                     /translation="MILKKGSLKKLPALLKVTFFNTLHTLPITVACMAVATLTANAIF
                     HLSGNAINVSIIYILAILLHARYTKCYSAGIIASLYSVFWVNYAYTYPYMTLNFTLSG
                     YPLTFVGMTFISCFTSSICIMLSKQSVQIQEKDRMLMQAEKETMRANLLRAMSHDLRT
                     PLTTIIGSSAAYLEQENEMSPEEKRKLIHNIEEDAQWLLNMVENLLSVTRIQDDKGIT
                     TVTKTDESLEEVISEAVRRFHKRFPGVTVQVHIPESFIMVPMDAMLIEQVINNLLENA
                     HFHSGSEKPIELKVRTENGVLYITIKDHGKGIDPKRLPTLFDGGGVNTNESGDSHKGM
                     GIGLSICKTIINAHGGTIQAANHADGAMFTFTLPDWKEY"
     gene            3588..4280
                     /locus_tag="ACZPCL_11015"
     CDS             3588..4280
                     /locus_tag="ACZPCL_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1163032.1"
                     /translation="MTTKANIIIIEDEKNICSFIEHILEPQGYRVISANTGKEGLQYI
                     TSEHPDVILLDLGLPDMDGLKLIEQVRSWSITPIIVISARTLEKSKIAALDLGADDYL
                     TKPFGTGELLARIRTALRHSQRNQHIGSSKYQVGDLLVDFERRLVKVKDQEIHLTQIE
                     YKLVCLLAQNAGRVMTYESIITKIWGPYADNDNQILRVNMAHIRRKLEENPAEPQYIF
                     TEIGVGYRMREE"
     gene            complement(4308..5258)
                     /locus_tag="ACZPCL_11020"
     CDS             complement(4308..5258)
                     /locus_tag="ACZPCL_11020"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR1163033.1"
                     /translation="MNYIVFDLEWNQSAEGKECSVEHMPFEIIEIGAVKLNSQMKQVS
                     EFHRLVQPKAYKEMHFKISEVTHMDMEELCKKGQPFPVVMEDFLNWCGEDEYVFCTWG
                     SMDLTELQRNMTYHGMEIPFPRPLLYYDVQKLYSLEYSDGKSRISLDHAVEQLELNQE
                     RPFHRALDDAVYTGRTLRMIHMETYGIYRSIDYYRLPERGSSYKLKFPEYSKYISGEF
                     DCKEEILKDKEISDIICVKCSRMLRKKIRWFSYGQRFYFALSICPEHGYMKGKIRVKK
                     SEQDLYYAVKTIKAVGEEALPMLVQKREDNKKKKKNSSKQ"
     gene            complement(5368..5438)
                     /locus_tag="ACZPCL_11025"
     tRNA            complement(5368..5438)
                     /locus_tag="ACZPCL_11025"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5404..5406),aa:Cys,seq:gca)
     gene            complement(5452..5523)
                     /locus_tag="ACZPCL_11030"
     tRNA            complement(5452..5523)
                     /locus_tag="ACZPCL_11030"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5488..5490),aa:Glu,seq:ttc)
     gene            complement(5550..5622)
                     /locus_tag="ACZPCL_11035"
     tRNA            complement(5550..5622)
                     /locus_tag="ACZPCL_11035"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5587..5589),aa:Asn,seq:gtt)
BASE COUNT         1586 a         1437 c         1159 g         1578 t
ORIGIN      
        1 actcttaatc tatttgtcca gggttcgagt ccctgaaggc gcacttaaag gtctgacttt
       61 gcagatacca gaagctgcgg ggttgggctt ttctttgtat ataaggaaat ttctctctga
      121 gagaacatat acttatcttc caaaaggagg caactactat gaaacacctt caacgatttg
      181 ccggtatcct tactgcattc tgccttatga tcatactttt tttcacttct gtagaagcag
      241 tggtctactg gactccggga tactttgaaa aagaatatac caaataccag gttctagacg
      301 accttccttc tatgactatg gatgacctct tagatgtaac agaccagatg atggcatatt
      361 taaagggaaa acgtgcagac cttcatgtta ccacccatat gggcggcgaa acccgtgagt
      421 ttttcaatga acgggaaatt gcccacatgg aagatgtaca gggactgttt ttaaaagcca
      481 ttgccctgcg ccggatctgc ctggtggtct gcgtcgttat gcttttattt atgatcctct
      541 caaaagcaga cctgcgcaca ttgcttcctt cttccctttg tataggaacc gggctgtttt
      601 ttgcagtaat cgcagcactg gctgctatta tatccaccaa ttttacaaaa tatttcatca
      661 tgttccacca catgtttttt accaatgacc tgtggatctt agacccgacc acagatatgc
      721 tgatcaatat cgtgcccgaa ggatttttca tggatactgc cggacgcatt gcttttctgt
      781 tcggcgccct gtcactgatc ctttttggca tctgcctgat catgacactg aaaaataaac
      841 gccgttaaca ggagctgctt ataatgaaac gaattctttc ttttatcctc agtatcaatc
      901 tgatctttat cctggttccc ggaaccgttc tggccgcgcc ttcctggccg tctgctgtct
      961 ctattgaagc agatggcggt attcttatgg aagcgcagac cggaacggtt ctttttgcta
     1021 aaaatgagga ccagccttat tatcctgcca gcattacgaa gatcctcact gcccttattg
     1081 tattagagca ctgcagttta gatgaagaag taaccttctc ccatgatgat gtttacaatg
     1141 tggaagcagg cagcagtaca gcaggtattg acgaaggaga tgtccttacg gtccgtgact
     1201 gcctgtacgc ccttatgctg gcttctgcca acgaaagtgc aaatgccctt gcctgccacg
     1261 taagcggctc cagagaagcc tttgcagagc ttatgaacca gactgcagaa agccttggat
     1321 gtacaggaag ccattttgcc aatccaagtg gtttaaacga tgaaaatcat tacaccactg
     1381 cccatgacat ggccctgatc accagagcag ccatccagaa cccggacttt ttagagatcg
     1441 atggcgcacg caactacaag cttccgccta ccaagcgcaa tttggaaggc ggttatgtag
     1501 ccaaccacca ccgtatgctg gtaaaaaaca catctgtgta ctatccaggg gcttttgccg
     1561 gaaagacagg ttacacttcc attgcaggca atacccttgt tacctgtgca gagaaaaacg
     1621 atatgaccct gatcgccgtt gttttaaatg ggcatcagac tcattattcc gataccaagg
     1681 ctctttttga ctttggtttt gatcgtttcc aggcgttaaa agcggtagac catgaaacct
     1741 cttaccaggc acttgataac gatatgacca tcggcggcat gactgcccag gacaatattt
     1801 ccctggagtt agacaaaaac agtcttgtga tcattccaaa agatgccgat ttctctgaca
     1861 cagaagcttc cctttcttac gaattggatg aaaatgcgcc ggatcatgct attgcccgca
     1921 ttttatatga ataccagggc cattccattg gacaggtcta tctgtgctct gctgcccata
     1981 cacaggcaaa tacagaaaac atatccgccg gatcacaggc actcgctgca cagacagaaa
     2041 ccggcactgc tgctcctgca gaaactcccg agcaaaagga aattcctgat gcaaatgccc
     2101 gttccagcgc acagacacgt tcctccgctt ccaaaaaggc atttccattc cttttagtgg
     2161 ggatctgctc tgcaaccctg atcattgtag gcgtgatcct ttttctcctt ctccattctc
     2221 acaggaagga aaaagaagat ctgctcctgc gccgtaagcg ccgtctggaa cgcctggaag
     2281 atatcggtta ttcttcttcc gattttgacc agcttctctc ccagaaaaga agttccactc
     2341 ctgcttacaa aaagcagaca cacaaagtaa agcggccccg taaaaaatgg taacactctc
     2401 ttacattgcg cttataagca gaatgaatta taatataatt atcctaatac tgaggtaaat
     2461 attatgattt taaaaaaagg ctctttgaaa aaactgcccg cgctattaaa agttactttt
     2521 ttcaatacac tgcacacact tcctataacg gtcgcctgta tggcggtagc tacgttgact
     2581 gccaatgcca tctttcatct cagcggtaat gccattaatg tatcgatcat ctacatcctg
     2641 gccattctgc tccacgcccg ttataccaaa tgctacagtg ccggtatcat cgcttctctt
     2701 tacagcgtgt tctgggtcaa ttacgcttat acttacccat atatgaccct gaattttaca
     2761 ttgagcggct acccgctgac attcgtggga atgaccttta tctcctgttt caccagcagt
     2821 atctgcatca tgctctccaa acagagcgta cagatccagg aaaaggaccg tatgctgatg
     2881 caggcggaaa aagaaactat gcgtgccaat ctgctgcgcg ccatgtccca tgacctgcgt
     2941 acccctctta ccacgatcat tggttccagc gctgcctact tagagcagga aaatgaaatg
     3001 tcaccggagg aaaagcggaa gctgatccac aatattgaag aagatgccca gtggctttta
     3061 aacatggtag aaaacctgct ttctgttaca cgcatccagg atgacaaagg cattaccact
     3121 gttacaaaaa cagatgaatc cctggaagaa gtgatctcag aggcagtcag acgcttccac
     3181 aaacgttttc ccggcgtaac tgtccaggtt catattcctg agtcctttat catggtcccc
     3241 atggatgcca tgctcattga gcaggttatt aacaatctct tagaaaatgc acattttcat
     3301 tccggcagcg aaaagcccat tgaattaaaa gtacgtacag aaaatggagt actttatatt
     3361 actattaaag accacggaaa aggtattgat cctaagcgac tcccaaccct ttttgatggc
     3421 ggtggcgtaa acacaaatga atccggcgac agccacaaag gaatgggaat cgggctttcg
     3481 atctgtaaaa ctattatcaa tgcccatggc ggtacgatcc aggctgcaaa tcatgcagat
     3541 ggcgccatgt ttacttttac cttacccgac tggaaggagt attaattatg actacgaaag
     3601 caaatatcat cattattgaa gacgaaaaaa atatctgcag ttttattgaa catattttag
     3661 agcctcaggg ctaccgcgtg atcagcgcca atacaggaaa ggaaggctta cagtacatca
     3721 cttccgagca tccggatgtg atcctgttag atttaggtct gccggatatg gacggcttaa
     3781 agctcattga acaggtccgt tcctggagca tcactccgat tatcgttatc tctgccagaa
     3841 cccttgaaaa gagcaagatc gctgctttgg atctgggcgc agatgactat ctcaccaagc
     3901 catttggtac aggagagctt ctggcacgta tccgtaccgc tctgcgccat tcccagcgca
     3961 accagcatat tggaagctcc aaataccagg taggcgatct gttggtggat tttgaacgcc
     4021 gtctggtaaa agtaaaagac caggagatcc atttaaccca gatcgaatac aaactggtat
     4081 gtcttcttgc ccagaatgca ggacgagtca tgacctatga gtctattatc acaaagatct
     4141 ggggacccta tgcagataat gacaaccaga tcctgcgtgt aaacatggcc cacatccgcc
     4201 ggaaactgga agaaaatccg gcagaaccac agtacatttt tacagagatc ggtgttggct
     4261 accgcatgcg tgaagaatag taaattaaaa agaagaattc gcccagccta ctgtttggac
     4321 gaattcttct ttttcttttt attatcttct cttttctgta ctagcatagg cagcgcctct
     4381 tctccaactg ccttgatcgt ctttactgca taatacagat cctgttccga tttcttcacc
     4441 cggatcttgc ccttcatata accatgttcc ggacaaatac tcagtgcaaa ataaaaccgc
     4501 tgcccataag aaaaccagcg gatctttttc cgcagcatcc ttgaacactt tacacagatg
     4561 atatccgata tttccttatc cttaaggatc tcttccttac agtcaaattc ccctgaaata
     4621 tatttggaat actccggaaa tttcaactta tagctgcttc cccgctcagg aagccggtaa
     4681 taatcaatgg atctgtatat cccatatgtt tccatatgga tcatacgaag ggtccggcct
     4741 gtatacaccg catcatccaa ggcccggtga aacggacgct cctggttcaa ttcaagctgt
     4801 tccactgcat ggtccaggga aatacggctc tttccatcag aatactccag agaatacagt
     4861 ttctgtacat cgtaatacaa taagggcctt ggaaacggta tttccatgcc atgataggtc
     4921 atattcctct gaagctctgt cagatccatc gatccccagg tacagaaaac atattcatct
     4981 tccccgcacc agttcaggaa atcctccatc acaaccggaa atggctgtcc ctttttacaa
     5041 agttcctcca tatccatatg ggtcacttct gatattttaa aatgcatttc tttatatgct
     5101 ttcggctgga ccagacgatg gaattcgctt acctgcttca tctgactgtt cagcttcaca
     5161 gctccaattt caataatctc aaaaggcata tgctccacag agcattcttt cccctccgcg
     5221 ctctggttcc actccagatc aaatacaata taattcatgt ctttatcctg tcaaattttt
     5281 tccttataca tagtaccata ttgacctcaa tatgaaaagg gcatatttca gacaaaaaaa
     5341 gaaccacatt tctgtgattc ttgagagagg cgacaaccgg aatcgaaccg gtgatagagg
     5401 tgttgcagac ctttgcctta ccgcttggct atgtcgccat atttagttaa atgaccccaa
     5461 ggggattcgg acccctgtta ccgccgtgaa agggcgatgt cttaaccgct tgaccatggg
     5521 gccttgctaa aaatataaac tttgtaaaac tccccgagta ggactcgaac ctacgaccca
     5581 acggttaaca gccgtttgct ctaccgactg agctatcgag gaatattgac ggtagaaggt
     5641 acataccttc aaaaccacat attgaactgt tgatcttccg aatacttaca acctttgttt
     5701 tccctaaacc tttttggtca agccctcgac cgattagtat cagtcagctc catgtgttac
//
LOCUS       DBMDUA010000170         5745 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5543_length_5745_cov_2.393338, whole genome shotgun sequence.
ACCESSION   DBMDUA010000170 DBMDUA010000000
VERSION     DBMDUA010000170.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5543_length_5745_cov_2.393338"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            211..1470
                     /locus_tag="ACZPCL_11040"
     CDS             211..1470
                     /locus_tag="ACZPCL_11040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1163034.1"
                     /translation="MKYAVVGFGTAGYHAVKKIRELDKEGCIDVYSNTGRAPYNPMLT
                     TYYAFGKLEYEVMFPFGDLEQIQKEVDFNLRREQVIKVHGSEHLVETENGSEQYDRIL
                     IATGARAFAPPVKGLEPEDAFLMRTVEDALRFKDRLKKNDIKHAVVIGASMAGIKVVE
                     VLHKYGIETWLLDLASYIFPLAAYKEVAKEIEKRVEAQGVHLMFGVTIDHMEQENGEK
                     TAVLTDGSRIPADIVGLCIGTRAITETVQGEVEIGRGIVVDDHMRTSVPGIYAAGDCC
                     EGNNLESGQNQIIGLWANANHQGETAGINMAGGDMAYKGNILHNITHFMNMDFIGFGD
                     NRMQGEVLEYGSLSEDLYIRLVLDGKRIVGANILDNYRVSGIVKNYMLRLFAGEQFVL
                     PDYQRAMLVKAGLSVPFIDEVEEKIHG"
     gene            1463..3598
                     /locus_tag="ACZPCL_11045"
     CDS             1463..3598
                     /locus_tag="ACZPCL_11045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR1163035.1"
                     /translation="MDSLEALKKAKIPGPETGIEKKNTVCAICSPAYNCGVCAYVKDG
                     KMVKLEGLDEHPRNKGCLCTKGLSGRGFAYREDRIKTPLRRIGKRGEGKFEPITWDEA
                     YKEIAEKLNHIKETDGPQAVAFFGGYNKWFRPWLRRFAHSFGTMNYGTESSTCMTAGW
                     MAWKTAIGQLAKPDMPNCDLLLGWAFNPYYSGYLKAQGAEKARARGMKVIVVDPKITP
                     TTEKMCDLHLRPYPGTDGALALCMGNVLIQKGWIDKEYIDKYVHGFKEYAQYAAGFNE
                     TNVEKLTGVPYELVVKACEMIHESKSMAINENSAPIPHHKNGFQNYRAIMALSALTGN
                     FDRKGGQLPGEHTFTHQIAGFTTLEDEFADGTEPKDAVLPVGAIRFPLWYHMEREMQC
                     VDLPRQIHEGTPYPVKALFAMGLNYRILPDDQYFKSAMEKLDFFVDADLFMTDAAKMA
                     DIVLPVCTSYERGELETYPGGYAWLTKPAIDRVGESKSDAEILCDLAKVMNLGDKRLE
                     EGYEKNIEEILSNLDITMEELRSSDLPVKVRGVKPYVPGTILEKGCKTPTGKFELYSE
                     LIASHPEWHLDALPTYCEPMDEADETVYPFILCSGGRLPNAIHSRLHKVPWVRSLRPD
                     PMADISIEDAQALGLKEGDDIELFTERGTISVKAHPTHRVQKNVVFFYHGYSEADVNS
                     LMSGTHVDPYSGFPAYNSTRCGMRKKVQR"
     gene            3595..4119
                     /locus_tag="ACZPCL_11050"
     CDS             3595..4119
                     /locus_tag="ACZPCL_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1163036.1"
                     /translation="MKKYAIDLDITKCISCGACSIACMDQNDFQVKEGQRPFRNVFDL
                     EMEQKGEVKYSHLSLACMHCSDAPCITACPSACLRKDPETNLTVYDTTNCIGCHSCAM
                     ACPYGIPSFGENGKMIKCDGCYVRVHHGMLPACVKACPVKALQLVDTEAKEPQIPPEH
                     SLRMTSAGMLKQQV"
     gene            4465..5148
                     /locus_tag="ACZPCL_11055"
     CDS             4465..5148
                     /locus_tag="ACZPCL_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR1163037.1"
                     /translation="MTRSQIRKYAFIKSIPIMCSYIFVSMAYGIMMEDAGFKWYYSLL
                     VSLTVYTGAFQFVLITFLSSGASIITVALTALLMNSRQSFYSLTFLEDFKKMGKRMLY
                     MIHTMTDETYAVNCTLELPQKEKEDTMFLVAFFSRCYWMIGSVLGGMLGQLIPWDMEG
                     IDFCMTALFVIIFIDQWEKADRHTPALAGLGIGIICLIIFGASGFMLPALLLTSGLLV
                     VLQKRKEEV"
     gene            5149..5481
                     /locus_tag="ACZPCL_11060"
     CDS             5149..5481
                     /locus_tag="ACZPCL_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter permease"
                     /protein_id="HIR1163038.1"
                     /translation="MSTERILIMIAVSALCTFFLRALPFLAFGGSQKMPDWLDRLGKT
                     LPSAIMAVLIVYCLKDVGSDVVHIGLPKIAAVVIVALSYKWKHNTLVSIAVGTAGYMI
                     LLRVAAML"
BASE COUNT         1709 a         1125 c         1438 g         1473 t
ORIGIN      
        1 ctcctatgtc agcctgtcca gcttaaatgc aagacagttc ctgtacattg gtctgttcat
       61 ccttgcagca gttgtactga tgttagttcg ctataagaac gtaaaagaaa aaatggatca
      121 gcagtaagca ctgtctgata ccgggaaaac gcggtggggt gttgcggcaa taacattctc
      181 accgcgttcc ctgttatggg aggtaagact atgaaatatg cagtagtagg ctttggtaca
      241 gccggctacc atgcagtgaa aaagatccgt gaactggata aagaaggatg cattgatgta
      301 tacagcaata caggtcgtgc cccttataat cctatgctga cgacttatta tgcttttgga
      361 aagctggaat atgaggtaat gtttcctttc ggcgatctgg agcagataca aaaagaagtg
      421 gattttaacc ttcgcaggga acaggtgatc aaggttcatg gatcagaaca tctggtagaa
      481 acagaaaatg gatcagagca gtatgaccgc attctgatcg caacaggcgc aagggcattt
      541 gctcctcctg taaaaggact ggagccagag gatgcatttt taatgcgtac agtagaagat
      601 gcccttcgtt ttaaagacag actaaagaaa aatgatatta aacatgccgt tgtgatcggc
      661 gcttccatgg ctggtatcaa ggtagtggaa gtccttcaca aatatggcat cgagacctgg
      721 cttttagatc tggcaagcta tattttccct ctggctgcct acaaagaagt agcaaaagag
      781 atcgaaaaga gagtagaagc ccagggcgtt catctgatgt ttggggtcac cattgaccat
      841 atggaacagg aaaacgggga aaagacagct gttttaacag atggaagccg cattcctgct
      901 gatatcgttg gcctgtgtat tggtacaaga gccattacag agaccgttca gggagaagtg
      961 gagattggaa gaggtattgt ggtagatgac catatgcgta cttctgttcc cggaatttat
     1021 gctgcaggtg actgctgtga aggcaacaac ctggaaagcg gccagaacca gatcattggt
     1081 ctgtgggcca atgccaatca ccagggtgaa actgccggta tcaacatggc ggggggagat
     1141 atggcctata agggaaatat ccttcacaat atcacccatt ttatgaatat ggattttatt
     1201 ggttttggag acaaccggat gcagggagag gtcctggaat acggttctct ttcagaagat
     1261 ctgtatatcc gccttgtatt agacggaaag cgcattgtag gcgctaatat tttagacaat
     1321 tatcgtgtca gcggcattgt taaaaactat atgctgcggc tgtttgctgg tgaacagttt
     1381 gttctgccgg attatcagcg ggctatgctg gtaaaagcgg gactttccgt tccttttata
     1441 gatgaagttg aggagaagat tcatggatag tttagaggct ttaaaaaaag caaagatacc
     1501 gggaccggag accggcattg aaaaaaagaa tacggtctgt gctatctgtt ctccggcata
     1561 caactgcggt gtatgcgctt atgtaaaaga tggaaaaatg gtaaaactgg aaggtctgga
     1621 tgaacatcca agaaataagg gatgtctatg tacaaaagga ctttccggcc gtggttttgc
     1681 ttacagagaa gaccgtatca agacacctct tcgccgcatt ggcaaaagag gagaaggaaa
     1741 atttgaaccg atcacctggg atgaagccta taaggagatc gctgaaaagc taaatcacat
     1801 taaagagaca gatggccctc aggcagtagc atttttcggc ggttacaata aatggttccg
     1861 tccgtggctg cgtcgttttg cccattcttt tggaactatg aactatggta cagagtccag
     1921 cacctgtatg acggctggct ggatggcatg gaaaacagcc atcggacagc tggcaaagcc
     1981 ggatatgcca aactgtgacc tgcttcttgg ctgggcattt aatccttatt attccggtta
     2041 cttaaaggct cagggagcag aaaaagcaag agcaagaggt atgaaagtca ttgtagtaga
     2101 cccaaagatc acgcctacta cagaaaaaat gtgtgatctg catctgcgtc cttatccggg
     2161 aacagacggc gctctggccc tgtgcatggg aaatgtactg atccagaaag gctggatcga
     2221 taaagaatac attgacaagt atgtacatgg ttttaaagaa tatgcccagt atgcagcggg
     2281 atttaatgag accaatgtag aaaagctaac aggcgttccc tatgagttag tggtaaaggc
     2341 ctgtgagatg atccatgaaa gcaagtccat ggccattaac gaaaacagtg cacctattcc
     2401 acaccacaaa aacggtttcc agaattaccg tgccattatg gcgctttcag ctttgactgg
     2461 caattttgac cgcaaaggcg gacagctgcc gggagaacat acatttactc accagatcgc
     2521 aggttttact accctggaag atgaatttgc cgacggcaca gagccaaagg atgcagtcct
     2581 tccagtagga gccatcaggt tcccgttgtg gtatcatatg gaaagggaga tgcagtgtgt
     2641 ggacctgcca agacagatcc atgagggaac accttatcct gttaaggcat tatttgcaat
     2701 gggcttaaat taccgcattc ttccagatga ccagtatttt aagtctgcca tggagaaact
     2761 ggactttttc gtagatgcag acctgtttat gactgacgct gcaaaaatgg cagatattgt
     2821 ccttccggtg tgcacctcct atgaaagagg agagttagag acctatccag gcggctatgc
     2881 atggctgacc aaacctgcca ttgacagagt aggagagagc aagtcagatg cagagatcct
     2941 ttgtgatctg gcaaaagtaa tgaatttagg ggataagcgt ctggaagaag gatatgagaa
     3001 aaatattgaa gagatcttat ccaacctgga tattactatg gaagaactcc gctccagtga
     3061 cctgcctgta aaggtaaggg gcgtaaaacc ttatgttccg ggaacgatcc ttgaaaaagg
     3121 ctgcaagact ccaactggta aatttgagtt atattcagaa ctgatcgctt ctcatccgga
     3181 atggcactta gatgcccttc caacttattg cgagccaatg gatgaagcag atgaaaccgt
     3241 atatccgttt attctctgct ccggcggaag attgccaaat gctatccatt cccgtctcca
     3301 caaggtaccg tgggtacgca gcctgcgtcc ggatcctatg gcagatattt ccatagaaga
     3361 tgcacaggca ttgggtttaa aagaggggga cgatatagaa ctgtttacag aaagaggaac
     3421 catttccgta aaagcacatc caacacacag agtgcagaaa aatgtagtct tcttctatca
     3481 tggatactca gaagcagatg taaactccct gatgtccgga acccatgtag acccatattc
     3541 cggtttcccg gcttataatt ctaccagatg tggtatgaga aagaaggtgc agcgatgaag
     3601 aaatatgcaa tagatctgga tattaccaag tgtatttcct gcggagcctg ctccatcgcc
     3661 tgcatggatc agaacgactt tcaggtaaaa gaagggcaga gacctttcag aaatgtattt
     3721 gacctggaaa tggagcaaaa aggagaggta aaatacagcc acctgtcttt agcctgcatg
     3781 cactgtagcg atgcgccttg cattaccgcc tgcccaagcg cctgtctgcg caaagatccg
     3841 gagaccaacc ttacagtata cgataccact aactgtatcg gctgtcacag ctgcgctatg
     3901 gcatgtcctt atggtatccc ttcctttggg gaaaatggca agatgattaa atgtgacggc
     3961 tgctatgtaa gagtccatca cggaatgctt ccggcctgcg taaaagcctg tccggtaaag
     4021 gcgctgcaat tagtagatac agaagccaaa gaaccgcaga tcccaccgga acattcttta
     4081 aggatgacaa gtgcgggaat gttgaagcag caggtgtaag taaaaataac tgcatccata
     4141 agccagaacc tattttacat gtgagaaaaa gatttgttat aatgatactg tcgcagatta
     4201 ggcatttttg tctggtctgt ggcagtattt ttattgccat ggatttgaaa aatggaaaat
     4261 attgtaaaaa taaacaggag aaggtcatat ggaaaaagct ctgaaaaaga gagaagccca
     4321 ggcagttgtg atcgaagaaa tgagaaggac cggaaagatc attccaagga tcggaaagac
     4381 gaaaagtcca cttacccatt gacaaagtag gaattaaaag atatgataag cgcaaagaaa
     4441 acggagtgaa aggacatcta cggtatgaca agatcacaga tccgaaaata tgcgtttatc
     4501 aaatccattc ccattatgtg cagctatatt tttgtcagta tggcttatgg tattatgatg
     4561 gaagatgcag gttttaagtg gtattattct ttgctggtga gcctgaccgt gtatacaggc
     4621 gccttccagt ttgtgctgat cacattttta agcagcggag cttctattat aacagtggct
     4681 ctcacagcac tgctgatgaa cagccgccag agtttttaca gccttacgtt cctggaagat
     4741 tttaagaaaa tgggaaaacg gatgctttat atgatccata ccatgacaga tgagacctat
     4801 gccgtaaact gtacgctgga actgccacag aaagaaaaag aggataccat gttcctggtg
     4861 gcatttttca gccgttgtta ctggatgatc ggttctgttt taggcggaat gttaggacag
     4921 ctgatcccgt gggatatgga aggcattgat ttctgcatga cagccctgtt tgtgatcatt
     4981 tttattgacc agtgggagaa ggcagacaga catacaccgg ctttagctgg tcttgggatc
     5041 ggcattatct gcctgatcat atttggggct tccggtttta tgcttccggc acttttactt
     5101 acatcaggat tattagtggt gttacagaag agaaaggagg aagtatagat gagtacagaa
     5161 agaatattga tcatgatcgc agtttctgct ctttgtacct tctttttgag ggctcttccg
     5221 tttctggctt tcggcggatc acagaagatg ccggactggc tggaccgatt gggaaaaaca
     5281 cttccctcag cgatcatggc agtgctgatc gtctactgtt tgaaagatgt gggaagtgat
     5341 gtggtacata tcggacttcc aaagatagca gcggtagtga tcgtagcgtt gtcttataaa
     5401 tggaagcata atacattagt aagtattgca gtaggaacgg ccggatatat gatccttctt
     5461 cgagtggcag ctatgttgta agattcatgc atctggcgga acagagaatg tatacatatt
     5521 ggaaaaacat atagaaagag catataaaaa agcggtgcag gaagcatatc caaatgatat
     5581 gtatccatgc atcgcttttt gctttttatg atcttccttc caactacagg aagaagattt
     5641 ttcgctttat aggaaagacc gccgccggcg ctcttttgaa ttaaaaatgc ccgccggtag
     5701 gcgggcaaca caaatagacg atctagggat taaaaaagat aaaaa
//
LOCUS       DBMDUA010000171         5720 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5573_length_5720_cov_3.549742, whole genome shotgun sequence.
ACCESSION   DBMDUA010000171 DBMDUA010000000
VERSION     DBMDUA010000171.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5573_length_5720_cov_3.549742"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            636..1481
                     /locus_tag="ACZPCL_11065"
     CDS             636..1481
                     /locus_tag="ACZPCL_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1163039.1"
                     /translation="MTTTGNSAGISGKRAQTQFRKYLLERNMSERTITAYCYALKQFY
                     GLYSQLTDKNLQLYKVFLIERYKPQTVNLRLRAMNCFVRYRESSLCPVSMVHVQQKGF
                     LEQIISQADYEFLKQRLWEDGEYNYYFLIRLMAATGVRISELVLFDVEDVKKGYRDIY
                     SKGNKLRRVYIPAVLRKAFEEWPGFSQRPDGILFLNRFGAPLSASGIRFQLKVFAARY
                     DIDPDVMYPHSFRHRFAKNFIEVCPDIALLSDLLGHDSIETTRIYLRRSSREQYRIVN
                     QVVNW"
     gene            1500..2384
                     /locus_tag="ACZPCL_11070"
     CDS             1500..2384
                     /locus_tag="ACZPCL_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1163040.1"
                     /translation="METTFIFPSDNTRFKENGSENTLSNDLELTPFKDFLLRKGASDN
                     TIRIYLLAVRHFFSHSDTIDPENLQAHKAWLLEHYRPATVNVHIHGMNQYLQYMEISS
                     FKLPAVKYQQKTFIDNVISKRNYEILKKKLKKDENWFWYFVVRFLGATGMRVSELLKI
                     KAEHVKLGMMDLYSKGGKMRRIYFPAALCEEAEIWLKRRDIVSGYIFTGRNGQVITAR
                     GIRSQLKVLAKRYKIPSEQVYPHSFRHRFAKNFLEKFNDISLLADLMGHESIETTRIY
                     LTRSSEEQQKLIDRVVKW"
     gene            2580..3644
                     /locus_tag="ACZPCL_11075"
     CDS             2580..3644
                     /locus_tag="ACZPCL_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1163041.1"
                     /translation="MYEYTWYQWLSFFYIYCFFGWIFESTYVSLKQHRFVNRGFLRLP
                     MLPLYGTGAVMMLWVSLPFKSSLVMVYISGVIGATILEYVTGWGMERLFKMKYWDYSN
                     QRFNLNGYICLSSSVAWGFLTIFLTEVIHKPIERWVLHVPTMIGIPCLSVITVVFIID
                     TAESVRTALDLARVLDAMTKMKAELDDVQVQLALLKAETEQKLEEAKEDTAVKLETLR
                     VEAAGKAAQLRNETAERAAQLKYETTERAAKLRLETALKAAQLKEHADEKAAQYREEA
                     AAKIEAAKNVKAAMTASRNERIAAMSSRMTELTKKRQDMMKHMNFYRRSILRGNPSAS
                     SMKFAAALKELREAAEKRNK"
     gene            complement(3953..5689)
                     /locus_tag="ACZPCL_11080"
     CDS             complement(3953..5689)
                     /locus_tag="ACZPCL_11080"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIR1163042.1"
                     /translation="MKDYMKIYQEWLSNPYFDEATKEELRAIEGNENEIKERFYMDLE
                     FGTAGLRGIIGAGINRMNIYVVRRATQGLANYIIKQGAANKGVAIAYDSRHMSPEFAM
                     EAAMTLAANGIKAYKFESLRPTPELSFAVRELGCVAGINITASHNPPEYNGYKVYWED
                     GAQFTPPHDKGVTEEVLAIEDLSTVKTTDEASATAAGLYQVIGQEIDDKYIAQVKAQV
                     VNQKAIDEMQDQITIVYTPLHGTGNIPARRVMKEIGFTHVYVVPEQELPNGDFPTVSY
                     PNPEAKEAFELGLKLAKEKDADLVLATDPDADRLGVYVKDTKSGEYIPLTGNMSGSLL
                     CEYVLSQKQAAGKIPADGQVVKSIVSTNLIDAVAKSYGAELIEVLTGFKWIGKQILKN
                     ETTGHGTYLFGMEESYGCLIGTYARDKDAISATAALCEAAAYYKQKGMTLWDAMIAMY
                     EKYGYYKDAVKAIGLSGIEGLAKIQSIMETLRNNTPTEVGGYKVTSARDYKLDTIKNM
                     ATGEVKPTGLPSSNVLYYDLEDGAWICVRPSGTEPKIKFYFGVKGTSLKDAEEKENAL
                     GAAVMAMVDKMM"
BASE COUNT         1504 a         1209 c         1316 g         1691 t
ORIGIN      
        1 cttatgtata aaagaaaacc gccctgtata aacaagacgg ttttgagcat tttcattcgg
       61 ttcattgacc tactccggtt tgaggtttca ctgcacaacc ccttttaaag actccgtata
      121 gcaaggcttt ccggtacgct gctatccttc aacgtctgga caaacacaca ctgttgtctg
      181 tctgatatta tcattatata gtgaggggag aattatgtca agaaaatcct caattttccc
      241 aactgtctac taatctaatt agacatgaaa gcattgaaac ctcgcggatc cacctgacaa
      301 aatctactga agaacagcga gatacactga ggcttttaga cctgtgtgag cagatccgca
      361 gacaagtgag tggtatagtt attttgaaaa cgatgtacta agattgtaat gtaggttttc
      421 tgcatgaata tatttatgtt ctcccggaat ctttctgtgc ctggttttgt gagaagattt
      481 tttgtcagtt atgcccacat ttctgaggcg tacgcggtgc gtacggtgat gaaatccggg
      541 tgcgactggc ggaaaaccgg ccacaaaggc aggtgcagga aagactccga gaggacatta
      601 tttcacttaa ggaggatatt ttaatgaaag aacagatgac aactacaggt aatagtgcgg
      661 gaatttcagg gaaacgggca caaacacaat ttcgtaaata tttattggaa cgaaatatgt
      721 cggaacggac gatcaccgct tattgttatg ctttgaagca gttttacggg ctgtattccc
      781 agctgacgga taaaaatctg cagctttata aagtatttct cattgaacgt tataaaccac
      841 agaccgtcaa tctgcggctt cgtgccatga actgttttgt acgttacagg gaaagctccc
      901 tgtgtccggt ctctatggta catgtgcagc agaaaggatt tttggaacag atcatcagtc
      961 aggcagatta tgagttttta aagcagcgct tatgggaaga tggggaatat aattattatt
     1021 ttcttatacg gcttatggct gctaccgggg tgcgtatcag cgaattggta ttgtttgatg
     1081 ttgaagatgt aaaaaaggga taccgggata tttattctaa agggaataag ctgcgcaggg
     1141 tttacattcc ggctgttttg cgaaaggcat ttgaggaatg gcccgggttt tcccagcgtc
     1201 cggatgggat cttattttta aaccggtttg gagctccctt atctgcttcc gggatccgtt
     1261 tccagttaaa ggtttttgca gcaaggtacg acattgaccc ggatgttatg tatcctcatt
     1321 ctttcaggca ccggtttgca aagaatttta ttgaagtgtg tccggatatt gccctgttat
     1381 cggatctttt aggacatgac agcattgaaa ccacccggat ctacctgcgg cgcagcagcc
     1441 gggaacagta ccgtattgtt aatcaggttg taaactggta gctgcagggg gtgtattgta
     1501 tggaaactac atttattttt ccctcagata ataccaggtt taaagaaaac ggatctgaaa
     1561 atacactatc caacgacctg gaactgaccc cttttaagga tttcctgctc cgcaaaggag
     1621 cttctgataa tacgatccgt atttatcttc tggctgtccg ccactttttc tcccattctg
     1681 ataccattga ccctgaaaac cttcaggctc ataaagcatg gcttttagaa cattaccgtc
     1741 ctgctactgt aaatgtacat atccatggca tgaaccaata tttacaatat atggaaattt
     1801 catcctttaa acttcctgcc gtaaaatacc agcagaagac ttttattgac aatgtaatat
     1861 ctaaaagaaa ctatgagata ctaaaaaaga agctgaagaa ggatgaaaac tggttctggt
     1921 attttgtagt acgtttttta ggagcaacgg gaatgcgggt cagcgaactt ttgaaaataa
     1981 aggcggagca tgtaaaactg gggatgatgg atctgtactc aaaaggcggt aaaatgaggc
     2041 gtatttattt tcctgctgct ctttgtgagg aagcggaaat atggctgaaa aggcgtgaca
     2101 tcgtttccgg ttatattttt acagggagaa atggacaggt gatcactgcc agaggtatcc
     2161 gttcccagtt aaaggtactg gcaaaacggt ataagatccc ttctgaacag gtttatcctc
     2221 actctttcag gcaccggttt gcaaagaatt ttctggagaa gtttaatgat atttccctgc
     2281 tggcagatct gatgggacat gaaagtattg agactacgcg gatctatctg acccggtctt
     2341 cagaagagca gcaaaaattg atcgaccggg tagtgaagtg gtagtacagg taaaagaaat
     2401 atggtatact catatcatga tgttgattcg taatccgctc atttttcttc aaaaaatggc
     2461 tccttactca tgtttttggc gggtcccaag ttcaaaaatc ctcctgcaag caagcttgcg
     2521 tcggatttcc ggccttttga ccctggtatc atatcattaa aacaaaggat ggacaaccta
     2581 tgtacgaata cacctggtat caatggctga gtttcttcta tatatattgc tttttcggat
     2641 ggatctttga gtctacctat gtatctttga aacagcaccg ttttgtaaac cgcggctttc
     2701 tgcgacttcc catgctgcct ctttatggta cgggagctgt gatgatgtta tgggtatctc
     2761 ttccatttaa gagcagtctg gtcatggttt acatctccgg tgtaatcggt gctacgatcc
     2821 tggaatatgt gacgggttgg ggaatggagc ggctgtttaa aatgaaatac tgggattaca
     2881 gcaaccagcg ttttaattta aacggatata tctgtctgtc ctcttctgtt gcatggggat
     2941 ttttgacgat ttttcttact gaggtgatcc acaagcccat tgaacgatgg gtgcttcatg
     3001 ttccaactat gatcggcatc ccatgtttgt ctgtgatcac agtggttttc attattgata
     3061 ctgcagaatc tgtacgtact gccctggatc ttgccagggt gcttgatgcc atgacaaaga
     3121 tgaaggcaga actggacgat gttcaggttc agctggcatt attgaaggca gaaacagaac
     3181 agaaactgga agaagcaaag gaagatacgg ctgtgaagct ggaaactttg cgtgtggaag
     3241 cagccggtaa agctgcgcag ctgcgaaatg aaactgcaga acgtgcagcc cagttaaaat
     3301 atgagacaac ggagcgggcg gcaaaactgc ggcttgagac tgcattaaaa gcggctcagt
     3361 taaaggaaca tgcagatgag aaagcagctc agtaccgtga agaggcggct gcaaagatcg
     3421 aagctgccaa gaatgtaaaa gctgctatga ctgccagccg taatgaacgt atcgcagcaa
     3481 tgagcagccg tatgacggaa ttaacaaaga aacgtcagga tatgatgaaa catatgaatt
     3541 tctaccgcag aagtatttta cgtggaaatc catctgcatc ttctatgaag tttgcagcgg
     3601 ctttgaagga attgagggaa gcggctgaga agagaaataa atagtaagcg tttatgtccc
     3661 cgctgatgcg gggatatatg ctgggtatta tcttcccgcc gatggcgggg agatgtcttg
     3721 gcagagcaaa cataaaaaca cccctgggat tgaaattttc tttcccaggg gtgtttacgt
     3781 ttgccaggta tccactcatg aaccccgcgc tgacgcgctc tactgtctgc ttacgcagac
     3841 ggttcattcg ttaatggtcc aagaaaaaca aatgtctgct tcgcagccgc ttgttttgcc
     3901 atgcatatcg agaatgcgcc tgtgacgcat tcgaagattt cttttggact cattacatca
     3961 tcttatcaac catagccatt acagctgcgc caagtgcatt ctctttctct tctgcgtctt
     4021 tcagggaagt tccctttacg ccgaagtaga atttgatctt tggttcggtg ccggatggtc
     4081 ttacgcagat ccatgcgccg tcttccaggt catagtagag aacgttggaa gaaggaagtc
     4141 cggtaggctt tacttcgccg gttgccatat ttttgatggt gtctaacttg tagtctcttg
     4201 cggaggttac tttgtagccg ccaacttcgg ttggagtatt atttctcagt gtctccataa
     4261 tggactggat ctttgcaagt ccttcaatac cggataaacc gattgccttt acagcatctt
     4321 tatagtagcc gtatttctca tacatagcga tcattgcatc ccataaggtc atgcctttct
     4381 gcttgtagta tgcagctgcc tcgcagagag cggctgttgc ggaaatagcg tccttatcac
     4441 gggcataggt tccgatcagg catccatagc tctcttccat tccgaaaagg tatgtaccat
     4501 gtccggtagt ctcattcttt aagatctgct ttccgatcca cttaaatccg gtcagaacct
     4561 cgatcagttc tgcgccgtat gattttgcta ctgcatcgat caggttggtg gaaacgatgg
     4621 atttaacaac ctgtccgtct gccgggatct ttccagctgc ctgcttctgg ctgagtacgt
     4681 actcacacag aagggatccg gacatatttc cagtcagtgg aatatattcg ccggacttgg
     4741 tatcttttac atatacacca agacggtctg catccgggtc agttgccagt accaggtctg
     4801 catctttctc ttttgccagc tttaagccca gttcaaatgc ttcttttgct tccgggtttg
     4861 gatagcttac agttgggaaa tcaccatttg gcagttcctg ctccggaact acgtatacat
     4921 gggtaaatcc gatctctttc ataactcttc ttgcagggat atttcctgtt ccgtgaagag
     4981 gtgtgtatac gatagtgatc tgatcctgca tctcatcaat ggccttctgg tttactacct
     5041 gagcctttac ctgagcgatg tatttatcat caatctcctg tccaattacc tgatacagac
     5101 ctgctgctgt tgcagaagcc tcatctgtag tctttactgt agacagatct tcgattgcca
     5161 gaacttcttc tgtaacacct ttatcatgag gaggtgtaaa ctgtgcgcca tcttcccagt
     5221 ataccttgta accattgtat tccggcgggt tgtggcttgc agtgatattg atacctgcta
     5281 cgcagcccaa ctcacgtact gcaaaggaca attccggtgt tggacgaagg gattcaaact
     5341 tatatgcctt gataccgttt gctgctaaag tcatagctgc ttccatagca aattccgggg
     5401 acatatgacg ggagtcgtat gcaattgcta cgcccttgtt agctgctccc tgcttgataa
     5461 tataatttgc aagtccctgg gtagcacgtc ttacaacgta aatattcata cggttgatac
     5521 ctgcgccgat gatacctctt aaaccagcgg ttccgaactc aaggtccata tagaaacgct
     5581 ctttgatctc gttttcattg ccctcaatgg cgcgcagttc ttcctttgta gcctcatcaa
     5641 agtaaggatt tgataaccat tcctggtata tcttcatgta atctttcatt ataacatact
     5701 ccctcctaaa ttaaattttg
//
LOCUS       DBMDUA010000172         5697 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5603_length_5697_cov_3.136347, whole genome shotgun sequence.
ACCESSION   DBMDUA010000172 DBMDUA010000000
VERSION     DBMDUA010000172.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5603_length_5697_cov_3.136347"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            134..1060
                     /locus_tag="ACZPCL_11085"
     CDS             134..1060
                     /locus_tag="ACZPCL_11085"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1163043.1"
                     /translation="MEIRQFTYVNMVAECGSMTKAAAKLFITQPALSNYISKLEEELG
                     TKLFDRSVTPMKLTFSGEKYLEHARTVMMQIDNMEREIRDITNHQTGRLRLGFPNERI
                     IYMLPALLPVFHEKYPGVRIETNNAPGVRLLDFLAAGDVDFVFLPGWEKRKGIVSHPI
                     SHEELILVAKKGYIPDECLLDSKMRIFNWDHISDFPLFTLKKGHALRSSLDLLCERTG
                     KIPNICMETHSNMLSCRLAAQGLGVALVPEISMEILKKDLDAEWYHLLECPVTWDVNI
                     YYREDAYLGTMEQDLIQMAEDVFGKRKLWKGV"
     gene            1177..2493
                     /locus_tag="ACZPCL_11090"
     CDS             1177..2493
                     /locus_tag="ACZPCL_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009216026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HIR1163044.1"
                     /translation="MKEGKGFLANFKKIGGMDLWMYAVCAVLVGATVTTGAMSNDLMA
                     YIAICLAVSLLLHRIGKVLPVWNTYIGGGLLMVFFGAALIKQFGLMPENYITMINDVT
                     NESTNTLTVFIVFLIMGSILSLDRGVLLRSFGGYIPAILGGLVGAAIAGTGVGLIFGV
                     SPVDALIRYVLPIMGGGNGAGAVPLSQVYEQVTGKPAAEYYSFAIIILTIANLMCIVA
                     GGLLNRLGKARPELTGDGTNIMKVDQKLIKEDVTADITMDDILGTLLLGGACYGVGRL
                     FSKVLLPTVLGAQIHSFAYTILFVVIIAALGIVPKNIRVTARKIQEFMTAVVAMMTMV
                     GLGFDFNLGELVAACSPGNIAIAFCVVIGAIIGSGLAGKLVGFYPIDSAVTAGLCMAN
                     RGGAGDVAVLTAADRMGLISYAQLSSRVGGGIVLIIASFAFSILLK"
     gene            2555..4525
                     /locus_tag="ACZPCL_11095"
     CDS             2555..4525
                     /locus_tag="ACZPCL_11095"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF005558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR1163045.1"
                     /translation="MGKSLTRKIIESHYVSGSMKAGEEVFIKVDQTLTHDINAVMTYL
                     AFEAIGLDRIRTECSVSYLDHNLLYVDHKTPDDHIYLQSAAERFGVHVSRPGNGICHA
                     VQVSRFGAPGKVSMGGDSHTPHGGSIGMLCIGVGGMDVATAMTGVPMRLKMPRVIKVN
                     LTGALKPGVNAKEVILEMLRRVTIKGGLGNVYEYAGPGAATLEVPQRATITNMGAELG
                     ATTSIFPADEQVRKFMKSQGREDEYRELLPDEDAEYDEVVDLDLSSLQPLVACPHQPD
                     NVKPLSEVEKLPVQQVFIGSCTNASYADIAKAALVFKGHKVNDNVSCTCAIASRQTYK
                     ALMEDGYLGMLMDAGVRILEIACGPCCAIGQTPATEGIAVRTSNRNFKGRAGNPNAKI
                     YLVSPESAAATAIMGTFATAADILGDQIDILAAVHEKDEYEINDNLIIKPLPEEEAKK
                     VEIVRGPNIKFLPVPEVPVQHLKVPVSLKGGDNISTDDITPASAEFSSMRSNIPLMSK
                     YCYHRYDPEFSERAREMGSSIIIGGENYGQGSSREHAAINPMYLGVKCVIAKSIARIH
                     KGNLVNHGIVPMLFEDPADYEKVDLRDELEIENFLDQIQAREVEVKDVTKGFNFKVKL
                     DLTDNELEVVLCGGQLRYLKRELVKQGVIEKE"
     gene            4742..5620
                     /gene="rarD"
                     /locus_tag="ACZPCL_11100"
     CDS             4742..5620
                     /gene="rarD"
                     /locus_tag="ACZPCL_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HIR1163046.1"
                     /translation="MDNTKRKGAVSVTITYICWGLLTVFWNLLAEVNSAYVLAQRVVW
                     SMLFMLGYLAVTGRWGDIRQVFADKKTMICCMVSGVLVCVNWGVYIFAINSGHVLDAS
                     LGYFLEPIFVTAIGVFLFHEKMSRLEQMTALFSIIGVGYLMGVYRMVPVMAMVIGLSF
                     AFYGAVKKSVHLDAGLSLFAETLFVTPIALLFCVYSEMNGHGSLGVLHGMQFLLFPMA
                     GVITSVPLLLFNKGVKMIPYYLTGILMYVNPTIQFLMGRFYFHEPLEIHRLIAFCFIW
                     GGVGFTIWHNLKEMRK"
BASE COUNT         1631 a         1119 c         1406 g         1541 t
ORIGIN      
        1 attttccttg gaaaaaagaa cataaaaatg tatgattaag gtatatatga ttatacagca
       61 atactctgga tgtgaaaaat agtttttttt aatagggcat ataagaagaa agaaataagc
      121 aaggagaaag aaaatggaga tacgtcagtt tacttatgtg aatatggtag cggaatgcgg
      181 cagtatgaca aaggctgcgg caaagctttt tattacccag ccggctctca gtaattatat
      241 cagcaagctg gaagaggaac tggggacaaa actgtttgat cgttctgtta cacctatgaa
      301 gctgaccttt tcaggggaaa agtatttaga gcacgccaga actgtgatga tgcagatcga
      361 caatatggaa cgtgagatcc gtgatattac aaaccaccag accgggcggc tgcgtctggg
      421 ctttccaaac gagcgtatta tctacatgct cccggcgctg cttcctgtgt ttcacgaaaa
      481 atatccgggg gttcgtattg aaactaataa tgcccctggt gtacgtttgc tggattttct
      541 ggcagccggt gatgtggatt ttgtattttt acctggctgg gagaagcgca aagggatcgt
      601 cagtcatcct atttcccatg aagagctgat ccttgtagca aaaaaaggat atattccaga
      661 tgaatgtctt ttagattcaa agatgcgtat ttttaactgg gatcatattt cagattttcc
      721 attgttcaca ttaaaaaaag gacatgccct gcgtagcagc ctggatcttc tctgtgaaag
      781 aacaggaaaa atacctaata tttgcatgga gactcacagc aacatgcttt catgccgtct
      841 tgcagcccag ggattaggcg tggcactggt accggagatc agcatggaga tactgaaaaa
      901 agacctggat gcagagtggt accatctgtt agaatgtccg gtcacctggg atgtaaatat
      961 ttattacagg gaagatgcgt accttggtac catggaacag gatctgatcc agatggcaga
     1021 ggatgttttc ggaaaacgta aactttggaa aggtgtctga aattaatcct caataagaaa
     1081 aagtgatagc ccccattaga caaaaggaat tttaccagaa ccccatattt ttctataatg
     1141 atgacagaag ttaagagaga aagaaagggg ttcaaaatga aagaaggaaa agggttttta
     1201 gctaatttta agaagatcgg gggaatggat ctttggatgt acgcagtttg tgcggtcctt
     1261 gttggagcca cagtgaccac aggagccatg agtaatgact tgatggctta catcgccatc
     1321 tgtctggcag tttccctgct gcttcaccgt atcggaaaag tgcttcctgt ctggaatact
     1381 tacattggcg gtggcctgtt aatggtattc tttggcgcag cactcatcaa gcagttcggt
     1441 ctgatgccgg aaaactacat aaccatgatc aatgatgtga ccaacgagtc caccaacaca
     1501 ctgaccgtat ttatcgtatt cctgatcatg ggttctattt tatctttaga cagaggagtt
     1561 ttacttagaa gctttggagg ttatattccg gctatcctgg gaggccttgt gggagcagct
     1621 attgcaggaa caggtgttgg actgatcttt ggagtaagtc ctgtagatgc cctgatccgt
     1681 tatgtccttc ccatcatggg cggtggaaac ggagctggtg cagtaccctt atcccaggtt
     1741 tatgagcagg taactggaaa gcctgcggct gagtattatt cctttgctat catcatcctt
     1801 accattgcaa acctaatgtg tatcgtagct ggcggccttt taaaccgtct tgggaaggca
     1861 agaccggaac taactggtga cggaaccaac attatgaagg tggatcagaa actgatcaaa
     1921 gaagatgtaa ctgcagatat tactatggat gatattttag gaactttatt attaggaggt
     1981 gcctgttatg gtgtgggacg tcttttcagc aaggtccttc ttccaactgt ccttggcgcc
     2041 cagattcatt cttttgcata caccattctt tttgtagtaa ttatcgcagc tttaggtatc
     2101 gttccgaaaa atatccgtgt aacagcaaga aaaatacagg aatttatgac agctgttgtg
     2161 gctatgatga ccatggtagg gcttggattt gactttaacc tgggcgaact ggttgcggcc
     2221 tgtagcccag gcaatattgc catcgctttc tgtgtggtta tcggtgccat catcggaagt
     2281 ggtctggcag gaaaactggt tggtttttat cctatcgact ccgctgttac agcaggtctt
     2341 tgcatggcaa acagaggtgg tgcaggagat gtggcagttt tgacagcagc agaccgtatg
     2401 ggactgatca gctacgcaca gctttccagc cgtgttggag gcggtatcgt tctgatcatt
     2461 gcatcctttg cattttctat tttattgaaa taataagcgc aggcagtata acacgcaagt
     2521 gcgtgttcgt gagtctaata caggaggcat aaaaatggga aaatcactta caagaaagat
     2581 cattgaatct cactatgtat ccggaagcat gaaagccgga gaagaagttt ttataaaagt
     2641 agatcagaca ctgacacatg atattaacgc agttatgact tatctggcat ttgaagccat
     2701 tggcctggac cggatccgca ctgaatgcag cgtaagctat ctggatcata accttcttta
     2761 tgtagatcat aagaccccgg atgaccatat ttacttacag tctgcagctg agcgcttcgg
     2821 tgtccatgtt tccagacctg gaaacggtat ctgccacgca gtccaggtat cccgtttcgg
     2881 cgctcctgga aaggtaagca tgggcggtga cagccataca cctcacggcg gttccattgg
     2941 tatgctgtgt atcggtgtgg gtggtatgga tgttgcaaca gccatgaccg gcgttcctat
     3001 gcgtctgaaa atgccaagag tcatcaaagt caacctgacc ggcgctttaa agccaggtgt
     3061 caacgcaaaa gaagtcattt tagagatgct tcgccgtgtg actattaagg gcggtcttgg
     3121 aaatgtatat gaatacgcag gtcctggcgc ggcaactctg gaagttcctc agagagcaac
     3181 tattaccaac atgggcgcgg aactgggcgc tactacctct attttcccgg cagatgagca
     3241 ggtaagaaag tttatgaagt cccagggaag agaagatgag tacagagaac ttcttccaga
     3301 tgaggacgca gaatatgatg aagtggtcga tcttgatctt agctccttac agccattagt
     3361 agcatgcccc caccagccgg acaatgtaaa acctctgtcc gaggtagaaa aacttcctgt
     3421 tcagcaggta tttatcggca gctgtaccaa cgcaagttat gcagatattg caaaggcagc
     3481 cctggtattt aagggacata aggtaaatga taatgtaagc tgtacctgtg ccattgcttc
     3541 cagacagacc tacaaagcat tgatggaaga cggttatctg ggaatgctta tggatgcagg
     3601 tgtacgtatc ctggagatcg cctgcggacc atgttgtgct atcggccaga caccggctac
     3661 agaaggcatt gcagtgcgta cttccaaccg taactttaag ggccgtgcag gtaatccaaa
     3721 tgcaaagatc tatctggtaa gcccggaaag tgcagcagcc actgcgatca tgggaacatt
     3781 tgcaacagca gcagatattt taggagatca gattgacatt ctggcagctg ttcatgaaaa
     3841 agatgagtat gagatcaatg ataacctgat cataaaacct cttccggaag aggaagctaa
     3901 gaaggtagag atcgtccgtg gaccaaacat caagttcctt ccagttccgg aagtaccggt
     3961 tcagcactta aaagtgccgg taagcttaaa gggcggcgac aatatttcca ccgatgacat
     4021 cacaccagcc agcgcagagt tttccagtat gcgttccaac atcccgctga tgtccaaata
     4081 ctgctatcac agatatgatc ctgagttctc tgagagagca agagaaatgg gctccagtat
     4141 catcattggc ggtgagaact atggtcaggg ctcttccaga gagcatgcag ccattaatcc
     4201 aatgtacctg ggtgtaaaat gtgtgatcgc aaagagcatt gcccgtatcc acaagggaaa
     4261 tctggtaaac cacggcatcg tgccaatgct ttttgaggat ccggcagatt atgaaaaagt
     4321 tgatctgaga gatgaactgg agattgaaaa cttcttagac cagatccagg caagggaagt
     4381 agaagtaaaa gatgtaacaa agggctttaa ctttaaggtg aaattagatc ttacagataa
     4441 tgagctggaa gtagttctct gcggaggcca gctgcggtat ctgaagagag aactggtaaa
     4501 gcagggggtt attgagaaag agtaattaca gataaagagc agaaatgttc atgtgacaaa
     4561 caaagataaa aaacagaaat gattgccata aaaaacatcg gtacacagga attcatcagc
     4621 ctggagtacg gatgtttttt gtgatcatat atcaaaaaat attttacaaa tcctgaaata
     4681 taaaataaac tagataaaaa cagatagaaa taaatacaaa agataacagg agagatcaca
     4741 catggataat acaaaaagaa aaggtgccgt cagcgttacg atcacctaca tctgctgggg
     4801 actgttgact gttttctgga acctgcttgc agaggtaaat tccgcctatg tactggccca
     4861 gagagtagtc tggtccatgc tttttatgct tggctatctg gcagtgaccg ggcggtgggg
     4921 agatatcaga caggtatttg cagacaaaaa gaccatgatc tgctgtatgg tcagcggtgt
     4981 tttagtatgt gtcaactggg gtgtatatat tttcgctatc aattcagggc atgtactgga
     5041 tgccagtctg ggatattttc tggaacctat ttttgtaaca gccatcggcg tatttttatt
     5101 tcatgaaaaa atgagccgtt tagagcagat gacagcactg ttttccatta tcggtgtagg
     5161 ttatctcatg ggcgtttacc gcatggttcc ggtaatggca atggtcatcg gtttaagttt
     5221 tgctttttac ggggcagtga agaaaagtgt gcatctggat gcaggacttt ccctgtttgc
     5281 agagacctta tttgtaactc cgatcgcgct gctgttctgt gtttacagcg aaatgaacgg
     5341 acacggaagt ctgggagttc ttcatggaat gcagttttta ctgtttccaa tggcaggtgt
     5401 gatcacatcc gttccattgc tgttatttaa taagggtgta aaaatgatcc cttattacct
     5461 taccggtatt ttaatgtatg tgaaccccac catccagttc cttatgggac gattttactt
     5521 ccatgaacca ctggagatcc accgcctcat cgctttctgc tttatctggg gaggagttgg
     5581 atttacgatt tggcataatt taaaagaaat gagaaaatag aaggatcacg aaaacatcat
     5641 attcgtaggg tggcatccac aatgccgctg tttcgccgtt cccatcatgg cggatta
//
LOCUS       DBMDUA010000173         5693 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5610_length_5693_cov_1.581187, whole genome shotgun sequence.
ACCESSION   DBMDUA010000173 DBMDUA010000000
VERSION     DBMDUA010000173.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5610_length_5693_cov_1.581187"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="ACZPCL_11105"
     CDS             <1..889
                     /locus_tag="ACZPCL_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1163047.1"
                     /translation="AFVRNDGSPMIAMMATLFSSLFNIVFDYILMFPFGMDMPGAALA
                     TALSPVVGILICQLHFRSKKCTVSFLPVIPSLKRLIFSCQVGVSAFVGEISSGVITTA
                     FNLIILRLAGNTGVAAYGVVANISLVAVALFNGISQGSQPLLSSFYGQADKKALEKVI
                     GLSLRTAAVTAVCIYAVIYLWSEPIAGIFNQEHNEELAAMARFGLKLYFIGFFFAGIN
                     IVGTGILSAVESVKWAFTASISRGFVMILLSAFTMSAAWGMTGIWLAFPAAEFITMGM
                     TFAGVWGFFSKVNLVGKSL"
     gene            1249..2424
                     /locus_tag="ACZPCL_11110"
     CDS             1249..2424
                     /locus_tag="ACZPCL_11110"
                     /inference="COORDINATES: protein motif:HMM:NF017495.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HIR1163048.1"
                     /translation="MIHDGFIYLGTLMLLAAILVNLPVYLKGKGAQKFFKFAPPIVLL
                     YLGMMLLCTMKMWNLEDTAATYKAVKNPLLYAMLFLMLLRCDLKKIIKLGPKMLIGFF
                     AASLSICTGFIVSYAIFHKMLGPDSWKALGALCGSWLGGSGNMLAVQAVLDVSEESMA
                     YSLVIDSVCAVMYVMFLLWVINFSKEFNAWTKADVRLINEVGASLEEEAKANTKPLTW
                     KSMLLLVGVSFFISAISKDVGSMVANALPVFDSATWTVLLVTAVGLLVAMTPFGKLKG
                     TEEVSNIILYVEIALIASRADISAMGNAPVWLAAGFLILLIHVAVMVILAKVIKMDIF
                     TCAVASLANVGGTATAPVLAGAYSSALVPVGILMALLGNIIGTPGGAFVGHLMSLIG"
     gene            2631..5066
                     /locus_tag="ACZPCL_11115"
     CDS             2631..5066
                     /locus_tag="ACZPCL_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR1163049.1"
                     /translation="MKHEELLSKLTPEEKAALLSGKTVWQTRDIPRLKIPSIFLSDGP
                     HGIRKQAGAGDHLGLNASLKATCFPTAATIANSWDPDLGEEIGKALGEEAAAQDVNIV
                     LGPGLNMKRSPLCGRNFEYFSEDPYLAGKMAAAYVRGIQSQGVYACPKHFAVNSQEER
                     RMAMDAVVDERTLREIYLTGFEIAVKEGHAKAIMTSYNEVNEEYANENKKLLVDILRK
                     EWGFDGLVVTDWGGSNDHVKGVMCQSDLEMPAPGLSSARELVTAVKEGCLSMEAFDLC
                     VDQVLDAVLNLSKKEETKKTDFDQKSHHALAKRAAAESAVLLKNEGNILPLKEKTKVA
                     LIGDFAFVPRYQGAGSSMVNPFEVESMDQLIHEYDLQVVSKVHGYTRDGEPDAALEKE
                     ALEAAAGAEVVLYCFGLDEISESEGKDREHMRLPQNQIRLLNELVKVNENIVGILSAG
                     SSIEMPWQHCLKAILHGYLGGEAGAGAILDILTGKINPSGRLAEAYPLRYEDTPAFRY
                     YPASERTSEYREGPFIGYRYYDTSLVRFQYPFGYGLSYTEFAYSALKADENGVQFTVT
                     NVGERDGAEVAQFYIGLPDSAVFRPGKELKGFQKVFLKAGESKTVHIPFDDKTFRYWN
                     VKTGRWEVEEGEYLIMVGACVADIRLSGKLWIAGTTSKMPYEKEKLPSYYSGLINNVG
                     AEEFEALLGHLAPSGKWQGELGLNDAICQMYYAKSRLARLVYRILADKKKKSEDKGKP
                     DLNVLFIYNMPFRGIAKMTGGAVSMEMVNGIVDMVNGKWGSGFRKVVKGYFHNKRENR
                     KYEKLLEGKEKQV"
     gene            5063..5572
                     /locus_tag="ACZPCL_11120"
     CDS             5063..5572
                     /locus_tag="ACZPCL_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR1163050.1"
                     /translation="MIAGNSWIRNWIDEHPAVWEFILFNVLSNVATIVNFIAMWICTG
                     FLFVSLENIPFQFFVFHYTQVESDLGLGGFLSFLVATAAAQAVNFYVQKNFVFRSNAA
                     FARAVPRYTCLAVVLVIVSAALPAYSQAFFKSLGLPQALIPTLANGVNIVVQVVLSYP
                     AMKFWIMPK"
BASE COUNT         1601 a         1044 c         1408 g         1640 t
ORIGIN      
        1 tgcctttgta cgaaatgatg gaagccctat gatcgctatg atggctacac tgtttagcag
       61 tctgtttaac attgtattcg actatattct gatgttccca tttggtatgg atatgccggg
      121 agcagccctt gcaacagctc tttcaccagt agtaggtatt ttaatctgtc agctgcattt
      181 tcgttcaaaa aagtgtaccg tatctttttt gccggtaata ccatcgttaa aaaggcttat
      241 tttttcctgt caggtgggag tttctgcttt tgtaggagag atatcttccg gtgtgatcac
      301 tacagctttt aatttgatca tattgcggtt ggctggaaat accggtgttg cagcctatgg
      361 tgtagtagcc aatatctctc tggtggcagt agctctgttt aacggcatat cccagggctc
      421 ccagcctctg cttagcagtt tctatggaca ggcagataaa aaggctttag agaaagtaat
      481 aggccttagt ttaaggacag cagcagttac ggctgtatgt atatatgcag tgatctattt
      541 gtggtcggaa ccaattgctg gtatttttaa ccaggaacat aacgaagaac tggcggctat
      601 ggctagattt ggtctgaagt tgtattttat tggtttcttt tttgccggta tcaatatagt
      661 aggaacaggc atattaagtg cggtagagtc agttaaatgg gcatttacag cttccatttc
      721 ccgtggtttt gttatgattc ttctttcggc ctttactatg tctgcagctt gggggatgac
      781 aggtatatgg ctggcttttc cggcagcaga atttattact atgggaatga ctttcgcagg
      841 tgtttgggga tttttttcta aagtaaatct tgttgggaaa tccctataag aaggctatag
      901 gaagtctttt tgagaaatcg ttaaaattga ctttatgtgc attataatct ataatatggg
      961 aggagaaagt caaaagaagt ggttaggggt agggaagtta caaaaattca agtgtaattc
     1021 acaagtccag aaaaatcttc tgggcctttt tttgcctttt agccagttct tatatcggta
     1081 ttataaaatg ttttcccgga gtctcttctc acaaaatcgg gcacaggaag attccggcag
     1141 aacataaaaa ggagaaggag gggcagtaaa atcataaccg gttattttga cttgctccta
     1201 agagaaaaag aggctgtcac aagatgacag cagaatggga gtttacaaat gattcatgac
     1261 ggttttatct atcttggcac tttgatgctg cttgcagcga ttttagtcaa tcttcctgta
     1321 tacctgaagg gaaaaggcgc acagaaattt tttaagtttg caccgcctat tgttttgctc
     1381 tacttaggta tgatgctttt atgtactatg aaaatgtgga acctggaaga tacagctgca
     1441 acttataagg cagtaaaaaa cccactgctg tatgcaatgt tgtttttaat gctgctgcgt
     1501 tgtgatctta agaagatcat caaattagga ccgaaaatgc ttattggttt ctttgcagcc
     1561 agcctttcta tctgtactgg ttttatagta agttatgcta ttttccataa aatgttaggt
     1621 ccggacagct ggaaagcgct gggggcattg tgtggaagct ggttaggcgg aagtggaaat
     1681 atgctggctg tacaggcggt tttagatgta agtgaggaaa gcatggcata ttcactggta
     1741 atcgactctg tttgtgcggt aatgtatgtt atgttccttc tgtgggtcat taacttttca
     1801 aaggaattta acgcatggac caaagcagat gtacgtctga ttaatgaagt tggcgcttcc
     1861 ctggaagagg aggcaaaagc gaacaccaaa cctcttacat ggaagagcat gcttctttta
     1921 gttggagtat ctttcttcat ttctgctatt tccaaggatg taggaagtat ggttgcaaat
     1981 gcactgcctg tttttgacag tgcaacctgg actgttcttc tggtaactgc tgtaggcctg
     2041 ctggtggcta tgactccatt tggaaaatta aaaggaacag aagaggtatc taatatcatc
     2101 ctttatgttg agattgccct gatcgcttcc agagcagata tttctgctat gggtaatgca
     2161 cctgtgtggc tggcagccgg cttcctgatc cttctgatcc atgtagctgt tatggtcatt
     2221 cttgcaaagg tgatcaaaat ggatatcttt acctgtgccg ttgcatcttt agcaaacgtt
     2281 ggtggaactg ctactgcacc tgtacttgca ggcgcataca gtagtgcttt agttccggta
     2341 ggtattctta tggcacttct gggtaatatt attggtacac cgggaggagc atttgtggga
     2401 catttaatga gtctgattgg atagtagtct ctgtcagata aggcagataa gtaaaaaggt
     2461 attccgggat catgaaaaat gatctgcgga ataccttttt taattcacgc cttgaagatt
     2521 tttctatttt tttgcttaaa aagtgtcata tggtaattag tttgatataa taggtgctgc
     2581 aaataaatgc caggaaatga aagaaataca gttattaaag aggaaatgat atgaagcatg
     2641 aggaattact ttcaaaatta acaccggagg aaaaggcagc tcttttaagc ggcaaaacag
     2701 tatggcagac ccgggacatt ccacgcctta agatcccgtc cattttcctt tctgacggtc
     2761 ctcacggcat ccgcaaacag gcaggagccg gggatcattt gggattaaat gcctctttaa
     2821 aggcaacctg ttttcctaca gcggcgacta tcgctaacag ctgggatccg gaccttgggg
     2881 aagagattgg aaaagcactg ggagaagagg cagcagccca ggatgtcaat atcgtactgg
     2941 gaccggggct taatatgaaa agaagtcctc tttgcggaag aaactttgaa tatttttccg
     3001 aagatcctta tcttgcagga aaaatggcag ctgcttatgt gcggggcatc cagagccagg
     3061 gagtgtatgc atgccccaaa cattttgcag taaacagcca ggaagagcgg cgcatggcta
     3121 tggatgcagt ggtagatgaa cggactctta gggagatcta cctgactggt tttgagattg
     3181 cagtaaaaga gggacatgcc aaagccatta tgaccagcta caatgaggta aatgaagaat
     3241 atgccaatga aaataaaaag cttctggtag acattttacg aaaggaatgg ggctttgacg
     3301 gactggtagt tactgactgg ggtggttcta atgaccatgt aaaaggggtc atgtgccagt
     3361 cagatctgga aatgccggca ccggggcttt cttccgccag ggagttagtt acagccgtaa
     3421 aagaaggctg tctttccatg gaagctttcg atctctgtgt agatcaggtc ttagatgcgg
     3481 tactaaatct tagcaaaaaa gaggaaacaa aaaagacaga ttttgatcaa aagtcccacc
     3541 atgcattagc aaaaagggca gcagcagaaa gtgcggtgct tttaaagaat gagggaaata
     3601 tccttccatt aaaagaaaaa acaaaggtgg ctctcattgg tgattttgcc tttgttccac
     3661 gttatcaggg cgccggctct tctatggtaa atccttttga agtagagtct atggaccagc
     3721 tgatccatga gtatgacctg caggtagtaa gtaaggttca tggatatacc cgtgatgggg
     3781 aaccggatgc agctttggaa aaagaagcct tggaagcagc tgccggggca gaggtggttc
     3841 tttactgttt tggacttgat gagatcagcg agtcagaggg gaaagaccgt gaacacatgc
     3901 gcctgccaca aaatcagatc cggctgttaa atgaattagt aaaggtaaat gaaaatattg
     3961 tgggtatttt aagtgccgga tccagtattg aaatgccttg gcagcactgt ttaaaggcga
     4021 ttcttcatgg ctatctgggc ggagaagcag gtgccggtgc gatcctggat atccttacag
     4081 gaaaaataaa tccttccggc cgcctggctg aagcctaccc tctgcggtat gaggatactc
     4141 ctgcattccg gtattatcct gcttcagaac gtacatcaga gtaccgggaa ggacctttta
     4201 taggataccg ctattatgat accagcctgg tccgcttcca gtatcccttt ggatatggac
     4261 tttcttatac agagtttgcc tattcagccc tgaaagcaga cgaaaatggg gtacagttta
     4321 ctgttacaaa tgttggagaa cgggacgggg cagaggttgc ccagttttat atcggacttc
     4381 cggactctgc cgtattccgc ccaggaaagg agttaaaagg ctttcagaag gtatttttaa
     4441 aggccggaga aagcaaaaca gtgcatattc catttgatga taaaactttc cgttactgga
     4501 atgtaaaaac aggacgctgg gaagtggaag agggcgaata tctgatcatg gtaggagcct
     4561 gcgtggcaga tatccgtctg tccggaaagc tatggatagc gggaaccaca agtaaaatgc
     4621 cttatgagaa agaaaaactc ccgtcctatt acagcggact gatcaacaat gtaggggcag
     4681 aggaatttga ggcacttctg gggcatcttg cgccttccgg taaatggcag ggcgaactgg
     4741 gacttaatga cgctatctgc cagatgtatt atgccaaaag ccgtctggcc cgtctggttt
     4801 accgtatact ggccgataaa aagaaaaaga gcgaggacaa aggcaaaccg gacttaaatg
     4861 tattatttat ctataacatg ccatttcgcg ggatcgcaaa aatgacaggc ggtgccgtaa
     4921 gcatggaaat ggtaaatggg atcgttgata tggtaaatgg taaatggggc tctggtttta
     4981 gaaaagtagt gaaaggctat tttcacaata agagagaaaa cagaaagtac gaaaaacttc
     5041 tggaaggaaa ggagaaacag gtatgatagc aggaaacagt tggatcagaa actggatcga
     5101 tgaacatcca gctgtctggg agtttatttt atttaatgta ttatccaatg tggcaactat
     5161 tgtgaatttt atagcaatgt ggatctgcac cggtttcctg tttgtatctt tagaaaacat
     5221 ccccttccag tttttcgttt tccattatac ccaggtagaa agtgatctgg gactgggcgg
     5281 atttttaagc ttccttgtgg caacagcagc agcccaggca gtgaatttct atgtgcagaa
     5341 aaactttgta ttccgctcca atgcggcgtt tgcaagagcc gtgccaagat atacctgcct
     5401 tgcagtagtc ttagtcattg tatctgcagc acttcccgct tacagccagg cattttttaa
     5461 aagccttgga cttccacagg cgctgatccc cacactggcc aatggagtca acattgtggt
     5521 acaggtagta ctcagctatc cggcaatgaa gttctggatc atgccgaaat aactttttaa
     5581 cattgctgtt gagtccataa ccaatacaga atataatttt attgaccctg ggagaaaatt
     5641 ccagggttat ttttatatta taagaaacca aaagttatac aaatcataac ttt
//
LOCUS       DBMDUA010000174         5656 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5649_length_5656_cov_2.455086, whole genome shotgun sequence.
ACCESSION   DBMDUA010000174 DBMDUA010000000
VERSION     DBMDUA010000174.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5656)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5649_length_5656_cov_2.455086"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            45..632
                     /locus_tag="ACZPCL_11125"
     CDS             45..632
                     /locus_tag="ACZPCL_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HIR1163051.1"
                     /translation="MENLVVYLTHRHDGPCVTQIKEGIKTVNKALYDMSLEREEFQGM
                     GCTLVAAVIEDGVLYAANVGDSRLYLIHDGSIRQITRDHSYVEEMVAMGMMQRGSADY
                     NRKKNIITRAAGIRPDIVPDFFEEELERGDYILLCSDGLSNMVDNDTLCSIILSEGSL
                     KEKAQRLIAEANERGGTDNIAVVLVKPEEGGMGSC"
     gene            626..2842
                     /gene="pknB"
                     /locus_tag="ACZPCL_11130"
     CDS             626..2842
                     /gene="pknB"
                     /locus_tag="ACZPCL_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HIR1163052.1"
                     /translation="MLNPGTYLQDRYEILGRIGSGGMSVVYKAKCHTLDRLVAIKVLK
                     EEFASDENFVSKFKMEAQAAARLSHPNIVNVYDVVDEGDLHYIVMELIEGITLKNYIE
                     KKGFLENKEAIGIAIQVAQGIAAAHEQHIIHRDIKPQNMIISKDGTVKVADFGIARAV
                     SSQTMNATAVGSVHYISPEQARGGYCDERSDIYSFGITLYEMVTGRVPFEGDNTVAVA
                     LAHLEEAVTPPSRLNPMVSRGLEEIILKCTQKKPDRRYPSIGDVITDLRHVMVDPDYD
                     IYGSGLETDEENDPSITRPISREELAQIQGRRRSRVEEAAVQEVEPEEDETEEPEDFS
                     DEDEYEEKSQPEQPTHKKRTVKKKEKDVSTQFERIIMVMGIVAAILVAVVVIFVFSRL
                     TGLFWSKTDQNKETEVVTSEEPTESINVSLADDQTYMPNVLGLPKDMAEAKLKEYNLV
                     MSVEAEDYSDNYKPDQVMRQDVAEGEVVEKWSTVGVTISKGSDKVDLTSLGLEQLSGE
                     DAQKLLIEKELKVTLKEESNDGVDTGKVIRYSPEQAKVGDTVELVVSTGPKTAQGQVP
                     RLLGKDEVTADALLKAAGLKTGTVTYDYDAGKPEGTVLTQSALPGTVLEPSSTVDYVI
                     NKMEEEGSTQAQAEENHYYIGSIDTTCSLSNYIGPASQTSSVKISIRLKQKDASGAEV
                     YTPLISPRLVVGSQDIPIVFPRIRGAYGVENGTVEVVDVEKDAVIASYPVSFFPVG"
     gene            3039..3917
                     /gene="rsgA"
                     /locus_tag="ACZPCL_11135"
     CDS             3039..3917
                     /gene="rsgA"
                     /locus_tag="ACZPCL_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR1163053.1"
                     /translation="MTGKIIKGIAGFYYVNNGENKVYQCKAKGVFRNRKIKPLVGDDV
                     EFSVLDEETMEGNIDEILPRKNELIRPAAANVDQALVLFALTHPAPNLNLLDRFLVMM
                     SMEDIPVKLCFNKQDLGDEVLMDQYRSIYEAAGYPVYFISAREETGIEQVRSLLRGKT
                     TVLAGPSGVGKSSLTNRIQPEADMETGDISRKIERGKHTTRHSQLFFVEKDTYMMDTP
                     GFSSMFTPDIEANELKDYFPEFAQYEDDCKFLGCVHIGEKVCGVKDAVAEGKISKSRY
                     DNYRLMYEELKQKRRY"
     gene            3920..4585
                     /gene="rpe"
                     /locus_tag="ACZPCL_11140"
     CDS             3920..4585
                     /gene="rpe"
                     /locus_tag="ACZPCL_11140"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR1163054.1"
                     /translation="MEYKVAPSLLAADFTRLGEQVHEIEEAGAPYLHLDVMDGAFVPS
                     ISFGMPVLKSLRKVTNMTFDVHMMVEEPGRYVEDIKKAGADIITVHAEACTHLDRVVN
                     QIKEAGCKAGVALNPATPVSVLECILGQVDMVLIMTVNPGFGGQKFIPYTLEKVKQVR
                     AMCDAKGLNTDIQVDGGVSAANVREVLEAGANVFVAGSAVFGSEPAARVKEFNEIFKE
                     YEK"
     gene            4582..5232
                     /locus_tag="ACZPCL_11145"
     CDS             4582..5232
                     /locus_tag="ACZPCL_11145"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIR1163055.1"
                     /translation="MKRCLILTGGKLSLAFAGSFLKEHKFEKVVAVDGGLEAAKELDI
                     IPDVIVGDFDTVHPEILAYYRKMEHIVWEVHQPEKDDTDTELAIKRALAMNCSHITLL
                     GATGGRLDHMVGNIHLLFPCLQKGTYAEIVDSQNRLYLIDGEHTFRKGETWGKYISFL
                     PLTEEVKGITLRGFKYPLTDRDISIGTSLCISNELVEEEGTITFSDGVLITVESHD"
     gene            5367..>5656
                     /locus_tag="ACZPCL_11150"
     CDS             5367..>5656
                     /locus_tag="ACZPCL_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1163056.1"
                     /translation="MNTAQAVQEKPEAKSNKVYKIALTGLFAALSYVVFTFLQFKIYL
                     PGGDATSIHLGNAVCVLGALLLGGFYGGLGGAIGMTIGDLFDPKYVFYAPKT"
BASE COUNT         1721 a         1088 c         1453 g         1394 t
ORIGIN      
        1 atgaatgatg tgtacccgca acaaacaggg atgaatagag tttggtggaa aacctggtgg
       61 tatacttaac ccaccgccat gacggtccct gtgttaccca gataaaagag gggataaaaa
      121 cagtcaataa agccctttac gatatgtctt tagagagaga agaatttcag ggaatgggat
      181 gcaccttagt ggctgctgtg atcgaagacg gtgttctgta tgcagccaat gtaggagaca
      241 gccgtttata tctgatccat gacggctcta tccgccagat cacccgggat cattcttatg
      301 tagaagaaat ggtggcaatg ggaatgatgc agagaggaag cgcggattac aaccggaaaa
      361 agaacattat tacccgggca gcaggcatcc gtcctgatat tgtgcctgat ttctttgagg
      421 aagaactaga acggggggat tatatccttc tgtgttccga tggactgagc aatatggtag
      481 acaacgatac cctgtgttcc attatccttt ctgaagggtc tttaaaagaa aaagcgcagc
      541 gtctcatcgc tgaggccaat gaaagagggg gaacagataa catagcagtt gtactggtaa
      601 aacctgagga aggagggatg ggatcatgtt aaatcctgga acctatcttc aggaccgtta
      661 tgagatattg ggacggatcg gttccggtgg tatgtctgtg gtttataagg caaaatgtca
      721 taccttagac cgcctggtgg ccattaaagt gttaaaagaa gagtttgcct ctgatgaaaa
      781 ttttgtcagt aaatttaaaa tggaagcaca ggcagcagcc aggctgtccc atcccaatat
      841 tgttaatgtt tatgatgtag tggacgaagg tgaccttcat tatatcgtca tggagctgat
      901 cgagggtatt accttaaaga actatatcga aaagaaaggt tttcttgaaa ataaagaggc
      961 cattggtatt gccatccagg tggcacaggg aattgccgct gcacatgagc agcatatcat
     1021 ccatcgtgat atcaagcctc agaatatgat catttcaaaa gatggaacgg tcaaggtagc
     1081 agactttggc attgcaaggg ctgtatccag ccagacgatg aatgcgacag cggtaggttc
     1141 tgtccattat atttcgccgg agcaggcaag aggcggatac tgtgatgagc gaagtgatat
     1201 ttattccttt ggcatcactc tttatgaaat ggtcaccggc agagtaccat ttgaaggaga
     1261 caatacagtt gctgttgctc ttgctcactt agaggaggca gttacacctc cttcccggtt
     1321 aaatccaatg gtaagccgcg gtttagagga gatcatttta aaatgtaccc agaaaaagcc
     1381 ggatagacgt tatccttcta ttggggatgt gatcacagat cttcgtcatg taatggtaga
     1441 tccggattat gatatttatg gatccggact ggagacagat gaggaaaatg atccgtccat
     1501 aacccggcct atttccaggg aagaactggc acagatccag ggacggcgca gaagccgcgt
     1561 ggaagaagca gcagtgcagg aagtggaacc ggaagaagat gaaacagaag aacctgagga
     1621 tttttcggat gaagatgaat atgaggaaaa atctcagcca gaacagccta cgcacaaaaa
     1681 gagaactgta aagaaaaagg aaaaagatgt aagtacccag tttgaacgga tcattatggt
     1741 catgggaatt gtggcggcta ttttagtggc agttgtggtc atttttgtat tttccagact
     1801 gaccgggctt ttctggtcca agacagatca gaacaaggaa acagaagtgg tcaccagcga
     1861 agaaccaaca gaaagtatca atgtttccct ggcagatgac cagacttata tgccaaatgt
     1921 cctgggactt ccaaaggata tggcagaagc aaaattaaaa gaatacaacc tggtgatgag
     1981 tgtggaagca gaagattatt ctgataatta caaaccggac caggtaatgc gccaggatgt
     2041 tgcagaaggc gaagtggttg agaagtggag caccgtaggc gtgaccatca gcaagggcag
     2101 cgacaaggta gatcttacat ctttgggatt ggaacagctt tccggtgaag atgcccagaa
     2161 gcttcttata gagaaagaat taaaagtaac cttaaaagaa gagtcaaatg atggcgttga
     2221 tacaggaaag gtcatccgct acagcccgga acaggccaaa gtgggtgata cagtagaact
     2281 ggtagtcagc acaggtccaa aaacagccca gggccaggtg ccaagattat taggcaaaga
     2341 tgaagtaact gcagatgccc ttttaaaggc agctggctta aaaacaggta ctgtgaccta
     2401 tgattatgat gcaggaaagc cggaaggtac agttctgacc cagtctgccc ttcctggaac
     2461 agttcttgag ccttcttcca cggttgatta tgtgatcaat aaaatggaag aagagggaag
     2521 cacccaggca caggcagaag agaaccatta ttacattggt tccattgata ccacctgctc
     2581 tttaagcaat tatatcggac ctgcttccca gacaagcagt gtaaaaattt ctatccggtt
     2641 aaagcagaag gacgcaagcg gggcagaagt ttatacccct ctgatcagtc cgagactggt
     2701 ggtaggttcc caggacattc ctattgtatt cccaaggatc cgtggggcat atggtgtgga
     2761 aaatggtact gtagaagtgg tagatgtaga gaaggatgca gtgatcgcct cttatccggt
     2821 ctctttcttc ccggtaggtt agggtaatgt cctctcagag tttttcatgc acctgctttt
     2881 gcggccgatt ttccgccagt cgcacccgaa tttcatcaac gtacgcaccg cgtacgcctc
     2941 agaaatttgg gcacaactga caaaaaatct tctcacaaaa tcagacacag aaagactccg
     3001 agagaacatt taaggtaaaa attgcgagga gaaagataat gacaggaaag ataatcaagg
     3061 gaattgcagg attttattac gtaaataacg gtgaaaataa agtataccag tgcaaggcaa
     3121 aaggtgtatt cagaaaccgt aagatcaaac ctctggtagg ggatgatgtg gagttttccg
     3181 tgctggatga agagacaatg gaaggaaata ttgatgagat ccttccaaga aagaatgagc
     3241 tgatccgtcc ggcagcagcc aatgtagatc aggcattagt cctgtttgcg ctgacccatc
     3301 cggcaccgaa cttaaattta ctggaccggt ttctggtcat gatgtctatg gaagatatcc
     3361 cggtaaaact gtgttttaat aagcaggatc tgggagatga ggtactgatg gaccagtacc
     3421 gcagtattta tgaggctgca ggttatcctg tatattttat cagtgccagg gaagaaaccg
     3481 gtattgagca ggtgagaagc cttcttcgcg gaaagaccac tgtattagca ggtccttcgg
     3541 gcgtgggaaa atcttctctt accaaccgga tccagccgga agcggatatg gaaacaggag
     3601 atatcagccg caagatcgag cgtggaaaac ataccacccg ccattcacag ctgttttttg
     3661 tggaaaaaga tacttatatg atggacacac cgggcttcag ctccatgttc acaccggata
     3721 tagaggcaaa tgagttaaag gattattttc cggaatttgc ccagtatgaa gatgactgca
     3781 agtttttagg ctgtgtccac ataggagaaa aagtatgcgg cgtaaaagac gcagtggcag
     3841 agggaaagat cagcaaaagc cgctatgaca attaccgcct gatgtatgaa gaactgaaac
     3901 agaaaaggag atattaacaa tggagtacaa agtagcaccg tcattactgg cggcagattt
     3961 taccagactg ggagaacagg tccatgaaat cgaagaagca ggagcacctt atctgcacct
     4021 ggatgtaatg gatggagcct ttgtcccaag tatttctttt ggaatgcctg tccttaaaag
     4081 cttaagaaaa gttaccaata tgacttttga tgttcatatg atggtggaag agccgggacg
     4141 ctatgtagaa gatattaaaa aagcaggcgc agacatcatt actgtacacg cagaagcatg
     4201 tacccatttg gaccgcgttg taaaccagat caaagaagca ggctgcaagg caggcgttgc
     4261 tttaaatcct gcaactcctg tatctgttct ggaatgtatc ttaggacagg tggatatggt
     4321 tttgatcatg actgtaaacc caggttttgg cggccagaaa tttatcccat atacattgga
     4381 aaaagtaaaa caggtacgtg ctatgtgtga tgcaaaaggc cttaataccg atatccaggt
     4441 agatggcggc gtcagcgcag ctaacgtaag agaggtactg gaagcaggtg ccaatgtatt
     4501 tgtagcaggt tctgctgtat ttggttctga accggcagca agagtaaaag aatttaatga
     4561 gatctttaag gaatacgaga aatgaaacgt tgtctgatct taaccggcgg gaagctttcc
     4621 cttgctttcg ccggttcttt tttaaaagag cataaatttg aaaaggtagt ggccgtagac
     4681 ggtggtctgg aggctgcaaa agagctggat atcatcccgg atgtgatcgt aggggatttt
     4741 gatacggtcc acccggagat actggcctat taccggaaaa tggaacacat cgtatgggaa
     4801 gtacatcagc cggaaaagga tgatacagac acagaacttg ccattaagcg ggctcttgcc
     4861 atgaactgca gccatattac gttattagga gctacaggag gaaggttaga tcatatggtc
     4921 ggaaatatcc atctgctgtt tccgtgcctg caaaaaggta cttatgcaga gatcgtagac
     4981 tcccagaacc gtctgtatct gatcgacggg gagcatacct tccgaaaggg agagacctgg
     5041 ggaaagtata tctctttcct gccccttaca gaagaagtaa aggggatcac gttaagaggc
     5101 tttaaatatc cattaacgga ccgggatatc tccataggca ccagcctctg catcagcaat
     5161 gaactggtgg aagaggaggg gacgatcact ttttcagatg gtgtattgat caccgtggag
     5221 tctcatgact gataagctgt tgtaaactgg actggtttaa aatatacaaa ctggatattt
     5281 aaccccatcc agcaaagtgc tagtatacct tctaatgaaa tgaccggata aatttttaac
     5341 agaaaaccgg tctgaggagg aaagttatga atacagcaca ggcagtacag gaaaaaccag
     5401 aggcaaagag caataaagtt tataagatcg ctcttaccgg actttttgcg gcattatctt
     5461 atgtcgtatt tacattttta cagtttaaga tctatcttcc aggcggagat gcaacttcca
     5521 tccatctggg aaatgcagtc tgcgttttag gcgcattgct tttaggcggt ttttacggtg
     5581 gtttaggcgg cgccattggt atgaccatcg gcgatctttt tgatcctaag tatgtgtttt
     5641 atgcaccaaa gacctt
//
LOCUS       DBMDUA010000175         5564 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5739_length_5564_cov_2.373421, whole genome shotgun sequence.
ACCESSION   DBMDUA010000175 DBMDUA010000000
VERSION     DBMDUA010000175.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5739_length_5564_cov_2.373421"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="ACZPCL_11155"
     CDS             complement(<1..647)
                     /locus_tag="ACZPCL_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HIR1163057.1"
                     /translation="MYIADLHIHSRYSMATSKFLTPEYLDLWARKKGIHILGTGDFTH
                     PLWRQELKEKLESAEPGLYKLKPELRLSEIPSGSFDPRFVVSGEISTIYKKNGRTRKV
                     HSLILLPGLEAADRLSGELEKIGNIHSDGRPILGLDCHDLLDMTLSVCPDAMYIPAHI
                     WTPHFSVFGAFSGFNSMEECFEELTPHIHAVETGLSSDPPMNWSVPSLSRYQLIS"
     gene            933..1280
                     /locus_tag="ACZPCL_11160"
     CDS             933..1280
                     /locus_tag="ACZPCL_11160"
                     /inference="COORDINATES: protein motif:HMM:NF045455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-fold beta flower protein"
                     /protein_id="HIR1163058.1"
                     /translation="MTLYNKKGKAAAYIENDVIWTFDGRAIAYLFQDAVYNFPGKQLG
                     WFEKGWLRDMGGYCVLFTPQAFAGPARPSMETTPAKYAKKALPSKGSREMRKAKMAYQ
                     AHWSEKEGIDTDV"
     misc_binding    1303..1553
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1620..4034
                     /gene="leuS"
                     /locus_tag="ACZPCL_11165"
     CDS             1620..4034
                     /gene="leuS"
                     /locus_tag="ACZPCL_11165"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR1163059.1"
                     /translation="MASQYNHSAIEKKWRENWEKNPINVNDGKKEKYYCLDMFPYPSG
                     NGLHVGHWRGYVISDVWSRYQLLKGKYVIHPMGWDAFGLPAENYAIKMGVHPAKSTAA
                     NIANIKRQIKQIAAIYDWDMEVNTTDPEFYKWTQWIFVQMFKKGLAYEKEFPINWCPS
                     CKTGLANEEVVNGCCERCGTPVTKKNLRQWMLKITAYAERLLNDLDKLDWPEKVKKMQ
                     TDWIGKSYGAEVEFPIEGRDEKITVYTTRPDTLYGATFMVLAPEHKLAKSLATDETRE
                     EVEKYIFDSSMRSNVDRMQAKEKTGVFTGSYAINPLNGAKTPIWLSDYVLADYGTGAI
                     MCVPAHDDRDFEFAKKFNLPIIQVIAKDGKEIENMTEAYTEASGTMINSGDWNGMESS
                     VLKKEAPHIIEEKGFGRKTVNYKLRDWVFSRQRYWGEPIPIIHCPKCGCVPVPEDQLP
                     LKLPEVESYQPTGTGESPLAAIDEWVNTTCPVCGAPSKRETNTMPQWAGSSWYFLRYV
                     DNHNSEALVSREKADKYLPVDMYIGGVEHAVLHLLYSRFYTKFLCDIGVIDFDEPFKK
                     LFNQGMITGKNGIKMSKSKGNVVSPDDLVRDYGCDSLRLYELFVGPPEQDAEWDDRGI
                     EGVSRFLNRFWNLVMDSKDKDVKETKEMVKLRHKLVYDIEQRFNQFSLNTVISGFMEY
                     NNKLMDLAKKEGGIDKETLKTFVILLAPFAPHIGEELWSQLGGTDSVFHSQWPECDTE
                     AMKDDEVEVAVQVNGKTRAVISVPADISKEDAIVQGKEAVKDKMAGNIVKEIYVPGKI
                     INIVCK"
     gene            4243..5031
                     /locus_tag="ACZPCL_11170"
     CDS             4243..5031
                     /locus_tag="ACZPCL_11170"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR1163060.1"
                     /translation="MKIQKKKWMTFAAAALIATGVLGMTSLAAGRADLNANTGGPGEK
                     ASVDVSTGEKPGDTQERQQDQQTAVITQILAVAGTGDCNADISYYKKENGNWELKWTE
                     KGYVGRNGITDNKKEGDGATPSGVYSFDLAFGLLDDPGSVLPYHKIAEGDFWVDDPAS
                     PHYNQLVNDKTTAKDWNSGEDLIKATPYYNYALNLDYNKERTPGEGSAIFLHCFKASG
                     YQGSSGCICLPESRMKELLGLVDTNTRIVIAKDAEHLNLSEYMK"
     gene            complement(5129..>5564)
                     /locus_tag="ACZPCL_11175"
     CDS             complement(5129..>5564)
                     /locus_tag="ACZPCL_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HIR1163061.1"
                     /translation="ISVFIDTFIVLNLTVFSILTTNSLSSGKDGIMLTQYAFSTVFGS
                     FGDIFIAVCLLFFAFSTILGWHFFGSINVKHLFGKKGMVPYSVLVAVFIVVGSMLKVD
                     LVWTLADFFNGLMVIPNALALLALSGVVASICKKYSSEKSDN"
BASE COUNT         1745 a         1115 c         1362 g         1342 t
ORIGIN      
        1 ttggagatca gctgatagcg gcttaaagat ggcacgctcc agttcattgg cggatcagag
       61 gaaagaccgg tttctacagc atggatatgg ggtgttaatt cctcgaaaca ttcttccata
      121 ctgttaaagc cggaaaatgc cccgaacaca gagaaatgag gggtccagat atgagcggga
      181 atgtacatgg catccgggca tacagaaagg gtcatatcta acaggtcatg gcagtccagt
      241 cctaaaatag ggcgtccgtc agaatgaata tttccaattt tttccagttc tcctgaaaga
      301 cggtctgcag cctcaagtcc tggaagcaga atcaggctgt gcactttccg tgtacggccg
      361 tttttcttat aaatggtgct gatctcaccg ctgaccacaa aacggggatc aaaggaaccg
      421 gaaggaatct cagaaaggcg caattctggt ttcagcttat aaagccctgg ctctgccgat
      481 tctaattttt cttttaactc ctgccgccac aaaggatggg taaaatcccc tgttccaagg
      541 atatggatgc ccttttttct ggcccacaga tccaggtatt ccggtgtgag gaacttgctg
      601 gtagccatag agtaacggga atgtatgtga agatctgcaa tgtacatgat aaaaatcctc
      661 ctgtttttct tgtgaaccca tacattatac cattgccttt caagagggga taggttaaaa
      721 taattttcga aagtattatg gggtgatgtc ctctcggagt ctttcatgca cctgcttttg
      781 tggccgattt tccgccagct gcacccgaat ttcatcaacg tacgcaccgc gtacgcctca
      841 gaaatttgag cacaactgac aaaaaatctt ctcacaaaat caggcacaaa aagattccga
      901 gagaacattg aattttacag gaggatgaga acatgacact ttataataaa aagggaaaag
      961 cagctgctta tatagaaaac gatgtgatct ggacctttga tggaagagcc atcgcctatc
     1021 tgttccagga tgcagtatac aatttcccgg gaaagcagtt aggctggttt gaaaaaggct
     1081 ggcttcgtga tatgggcgga tattgtgttt tatttacccc tcaggcattt gcaggacctg
     1141 caagaccatc catggagaca actcctgcaa agtatgcaaa aaaggccctg ccatctaagg
     1201 gttcaaggga aatgagaaag gcaaaaatgg cttaccaggc tcattggagc gaaaaagagg
     1261 gtattgacac tgatgtgtaa atatgctaaa ttatggttta gtgcaaatgc agtgatatgg
     1321 aagtagtaaa cactttttaa gccccacaga gagccgggaa aagatgagaa ccggtgactg
     1381 gaaagtgccg aactgatcca tgagcagctt ttcagaaaga gatgagatca ttacaggatc
     1441 ttcattttca gtaagaaaag acggaggcct taccgttata agaggaattg agtgcatgcg
     1501 acttcgcatg aatcagagtg gtaccgcgtg gaaatgatat ctgcgtctct tggaaacagg
     1561 agacgcagtt tttttatctc attacgtcac ccacggcaat atttacggag gaatgaatta
     1621 tggcatcaca gtataatcac tccgccattg agaaaaaatg gcgtgagaac tgggaaaaga
     1681 accccatcaa tgtaaatgat ggaaagaaag agaaatatta ctgcctggat atgttcccat
     1741 atccatcagg aaatggtctt catgtaggac attggagagg ctatgttatc tctgatgtat
     1801 ggagcagata tcagctgtta aagggcaaat atgtgatcca tcctatgggc tgggacgcat
     1861 ttggtctgcc tgcagaaaac tacgcgatta agatgggtgt acatcctgca aaatctacag
     1921 cagctaacat tgcaaatatc aaacgtcaga tcaagcagat cgctgcgatc tatgactggg
     1981 atatggaagt aaataccaca gatcctgaat tctacaagtg gacccagtgg atcttcgttc
     2041 agatgttcaa gaagggtctt gcatacgaga aggaattccc aatcaactgg tgcccatcct
     2101 gtaagacagg tctggctaac gaggaagttg taaatggctg ctgcgagcgc tgcggcactc
     2161 ctgttaccaa gaagaacctg cgtcagtgga tgttaaaaat cactgcctat gcagaaagac
     2221 tgttaaatga cctggataag cttgactggc ctgaaaaggt taagaagatg cagactgact
     2281 ggatcggaaa atcttatggt gcagaggttg agttcccaat cgaaggcaga gatgaaaaaa
     2341 tcactgttta caccacaaga cctgataccc tgtatggtgc aaccttcatg gtactggctc
     2401 cagagcacaa gctggctaag agccttgcta cagatgagac cagagaagaa gttgagaagt
     2461 acatcttcga ttcttccatg cgttctaacg tagaccgtat gcaggcaaag gaaaagaccg
     2521 gcgtatttac cggaagctat gctatcaacc cattaaatgg cgcaaagaca ccaatctggc
     2581 tgtctgacta tgtattagct gattacggta ccggcgctat catgtgcgta cctgctcatg
     2641 atgacagaga ctttgaattt gctaagaaat ttaatcttcc gatcatccag gttattgcca
     2701 aggatggcaa ggaaattgaa aatatgaccg aagcttatac agaagcaagt ggaaccatga
     2761 tcaactccgg cgactggaac ggcatggaat cttctgtatt aaagaaggaa gctccacaca
     2821 tcatcgagga gaagggcttc ggccgcaaga ctgtaaacta caagctgcgt gactgggtat
     2881 tctcccgtca gcgttactgg ggcgagccga tcccgatcat ccactgcccg aagtgcggct
     2941 gtgtaccggt accggaagat cagcttccat taaagctgcc tgaggtagaa agctatcagc
     3001 ctacaggaac cggtgagtca ccactggctg caattgatga gtgggtaaat accacctgcc
     3061 cagtctgcgg tgctccttct aagagagaga ccaataccat gcctcagtgg gctggttctt
     3121 cctggtactt cctgcgttat gtggacaacc acaacagcga agctctggtt tccagagaaa
     3181 aagcagacaa gtatctgcca gttgatatgt acatcggcgg cgtagagcat gcagttcttc
     3241 acttactgta ttcccgtttc tacaccaagt tcctgtgcga tatcggtgta attgactttg
     3301 acgagccatt taagaagctg ttcaaccagg gtatgatcac tggtaagaat ggtatcaaga
     3361 tgagtaagtc caagggcaac gtagtttccc cagatgatct ggtaagagat tacggctgcg
     3421 actccttaag actttacgaa ctgttcgtag gaccgccaga acaggatgca gagtgggatg
     3481 acagaggtat tgaaggtgta tcccgtttcc tgaaccgttt ctggaacctg gttatggaca
     3541 gcaaggacaa agatgtgaaa gaaaccaagg aaatggttaa gctgcgtcat aaactggttt
     3601 acgatatcga gcagagattt aaccagttca gcttaaatac tgttatttcc ggtttcatgg
     3661 aatacaacaa caagttaatg gatctggcta agaaggaagg cggaattgat aaagaaacct
     3721 taaagacctt cgttatcctg ttagctccat ttgcaccaca tatcggtgaa gaactgtgga
     3781 gccagttagg cggaaccgac agcgtattcc attcccagtg gccggagtgc gatactgagg
     3841 ctatgaagga tgatgaagta gaagtagctg ttcaggtaaa tggcaagacc cgtgcagtta
     3901 tcagcgtacc ggctgacatc tccaaggaag atgcgattgt tcagggcaag gaagcagtaa
     3961 aggataagat ggctggaaat atcgtaaaag agatctacgt tccaggaaag atcatcaaca
     4021 ttgtttgcaa gtaattagaa ttgttgaaat aattgaaata gttcaaattg cagatacaaa
     4081 gaggcatcgg tgtataaccg gtgccttttt gctgtccagg ctttcatttt tgcatcaggg
     4141 taagaaaaat gtaagagaaa cgaagctgta aatgtggtaa gataagggat agaaaaatga
     4201 aatggtaaga tgaaacagta aaaaggaagg atcgtacata aattgaagat acagaagaaa
     4261 aaatggatga cctttgcagc agcagctctt attgccacgg gagtattagg catgacttcc
     4321 ctggccgcag gaagagcaga cttaaatgca aataccgggg gaccaggaga gaaagcctct
     4381 gtggatgttt caaccggaga aaagccgggt gacactcagg aacgtcagca ggaccagcag
     4441 acagctgtga tcacccagat ccttgcagta gccggaaccg gtgactgcaa cgcagacatt
     4501 tcttattata agaaggaaaa tggaaactgg gaattaaagt ggacagaaaa aggctatgta
     4561 ggaagaaatg gcattacaga caataaaaaa gaaggagacg gagcaactcc ttccggcgtg
     4621 tactcctttg atctggcttt tggtctttta gatgatccgg gaagtgtact gccataccat
     4681 aagatcgcag aaggtgattt ctgggtagat gatccggcca gtcctcatta taaccagctg
     4741 gtaaatgata agactacagc aaaagactgg aattcagggg aagatctgat caaagcaact
     4801 ccttattata attatgcttt aaatctggat tataataagg aaagaacacc aggagaaggt
     4861 tctgctattt tccttcactg ttttaaagca tctggttatc agggctcctc agggtgcatc
     4921 tgcctgccgg aaagccggat gaaggaatta ttaggattgg tagatacgaa caccaggatc
     4981 gtgatcgcaa aggatgcaga gcatctgaat ttaagtgagt acatgaagta agaaatggat
     5041 caaaaagggg cgcccgcaga ggcgcccttt taatattata cgatattata cgatcataca
     5101 agaacacgca gatagcgcgt ttgctgatct agttatctga tttttcagaa ctgtatttct
     5161 tacagataga agcaacaaca ccgcttaaag ccaacagagc cagggcatta ggaatgacca
     5221 taagaccgtt aaagaagtcg gccagtgtcc aaaccagatc cacttttaac atagaaccaa
     5281 caacgataaa cacagctacc agaacagaat aaggaaccat tcctttcttg ccgaataagt
     5341 gttttacgtt gatagaacca aaaaagtgcc agccaaggat ggtggaaaag gcaaagaaca
     5401 gaaggcatac ggcgatgaaa atatcaccga aggagccgaa aacagtagaa aatgcatact
     5461 gggtcagcat aataccgtcc ttgccgctgc ttaaagagtt agtagtaagg atactgaata
     5521 cagttaaatt taatacgata aaggtatcaa taaatacgct gatc
//
LOCUS       DBMDUA010000176         5546 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5756_length_5546_cov_2.056749, whole genome shotgun sequence.
ACCESSION   DBMDUA010000176 DBMDUA010000000
VERSION     DBMDUA010000176.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5756_length_5546_cov_2.056749"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(144..284)
                     /locus_tag="ACZPCL_11180"
     CDS             complement(144..284)
                     /locus_tag="ACZPCL_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163062.1"
                     /translation="MKLRRVLGSFAWVALAAALILVNAAAANAQDKAVEDGQTVQTIT
                     VK"
     gene            472..735
                     /locus_tag="ACZPCL_11185"
     CDS             472..735
                     /locus_tag="ACZPCL_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1163063.1"
                     /translation="MINYQPFWETLKASGESTYSLIHDYSISSATIDRLRKNRPLSTS
                     TINDLCIILGCSPENILAFTPPSPETKENDIFLKHRSLPRTRE"
     gene            852..1724
                     /locus_tag="ACZPCL_11190"
     CDS             852..1724
                     /locus_tag="ACZPCL_11190"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HIR1163064.1"
                     /translation="MQKIGRNDPCWCGSGHKYKNCHMDFDVKLSEYRHKGSKVPSHAM
                     IKNPEQIAAIRESAKINVSVLDYVAEHICAGISTEQIDLWVYEQTTHRGGIPAPLNYE
                     GFPKSVCTSVNDQVCHGIPSADVILKDGDIINVDVSTIYKGYYSDSSRMFCIGEVSPE
                     KKRLVEITKQAIEEGLKQVRPWGHLGDMGQAINDYATANGYTVVREIGGHGVGLEFHE
                     DPWVSFVSKKGTEMLMVPGMIFTIEPMINMGKADIFVDAKNDWTIYTDDGMPSAQWEI
                     QVLITEDGYEILSW"
     gene            complement(1816..3216)
                     /locus_tag="ACZPCL_11195"
     CDS             complement(1816..3216)
                     /locus_tag="ACZPCL_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HIR1163065.1"
                     /translation="MKYSKGKVSDIQIAYIGGGSRGWAWTFMTDLALEDEISGTIRLY
                     DIDRKAAQTNEVIGNHLMNRPEAKGDWKFEVKDSLKEVLTGCDFAVISILPGTFDEMA
                     SDVHLPERLGIYQSVGDTAGPGGIIRGLRTIPMFVEIAEAIKKYAPDCWVINYTNPMS
                     LCVKTLYHVFPQIKAFGCCHEVFGTQKVLRGIYEEETGDKIADWHDIHVNVVGINHFT
                     WFTEASYKGVDLFPVYKNYIDHHFEEGYQLEGENWMNSSFKCANRVKMDLFCRYGLIA
                     AAGDRHLAEFMPGNEYLNDPETVASWKFGLTTVDWRKEDLKKRLERSHRLAAGEEEID
                     LTPTGEEGILLIKALCGLGRVVSNVNIPNTAGQIPNLPENALVETNALFDRDSIHPVA
                     AGKVPENVKALLMPHIENHERTLKAALTCDEELVVQAFLHDPVVKGKQPQEAQVRSLV
                     HDMIENTRKYLPDGWN"
     gene            3402..4358
                     /locus_tag="ACZPCL_11200"
     CDS             3402..4358
                     /locus_tag="ACZPCL_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1163066.1"
                     /translation="MEQDTLYLSFSEIPDASEILFKGEIPGIFVEEAIRSHSFSMVTR
                     HFHESLELYFLIEGERFYFVEQDTYHLQEHMAILVDQNQIHKTCPAGTGKKHHRFLMQ
                     LEGPVLDQLLRLCGSVSLEEFGNAYRGITRFSVDQWAQILSLLTQIKEQFTLISSRTT
                     PGNKQDTMTANGLIRLYTVQLLLLLIQIRRTQEEGGWKENLQNNLVHTGMYQQVHDIA
                     IYLQNHCSEKNSLDQIAAHFFISRSYLTRIFRKVTGFTITEYQNICRIKKAQLLLRET
                     ELSMTDIALEAGFINLPYFERVFRKTTSLTPLQYRRQMRPYS"
     gene            complement(4337..>5546)
                     /locus_tag="ACZPCL_11205"
     CDS             complement(4337..>5546)
                     /locus_tag="ACZPCL_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HIR1163067.1"
                     /translation="GTLWKQCQPVLCPDSDEDAHGVYSGSAFCENGKINFFYTGNVKL
                     FDRPDYDYINSGRVSNTMYVTSEDGMHFSPKKCLMTNSDYPADISAHVRDPKIIKRED
                     GYYMVLGARDKESKGLVLVYRSDDLTNWKYHGRITTENAFGYMWECPDLFELDGQLCL
                     ICCPQGVEKKGYDYWNVHQCVCIPLDYDFTPGTWVIKTTDNFQMVDRGFDFYAPQTFA
                     DQQGRRILIGWMGIPDADYTNPTVKKGWQHALTIPRQLHIRDGKLYQEPVKELEKLCR
                     NGRTFSKEEFQNLELPKCAKVQIDFKDCKNMEFSISDEVKLTYMDHVLTLDLGNCGSG
                     RTIRKVWIEELTDLTLFADTTSLELFINKGSQVFTTRVYGKQGKAGLKGQCDARIQVY
                     EMSGYEYGRI"
BASE COUNT         1486 a         1371 c         1077 g         1612 t
ORIGIN      
        1 cttttttaat ccctagatcg tctatttgtg ttgcccgcct accggcgggc atttttaatt
       61 caaaagagcg ccggcggcgg tctttcctat aaagtaaaaa aagagccgca cgctgcgact
      121 ctgtacccgg actcatgtcc ctcttatttt actgtgatcg tctgaacggt ctgaccgtcc
      181 tctacggctt tatcctgcgc attggctgcc gctgcattta caaggatcag cgcagctgcc
      241 agggccaccc aggcaaaact acctaaaact cttcttaatt tcatggtact cttcctccga
      301 accccattat acataagttt tacaaaaatg caagattttt tcattttttt acagaaggac
      361 ggggattttt tacattctct tacgtttatt atttataggt aaaaatacag caacaatgtc
      421 aaacatacaa aaataaaata aactactata acaataacag aaaggacctt tatgataaac
      481 tatcagccct tctgggaaac actgaaagcc agcggagaat ctacttattc gctgatccat
      541 gattattcca tctccagtgc taccattgac cgtttgagga aaaaccgacc attaagtacc
      601 agcaccatca atgatctttg tatcatttta ggctgcagcc cggaaaacat ccttgctttt
      661 acgcctcctt cccctgagac taaagaaaac gatatcttcc taaaacacag atctctgccc
      721 cgtacaaggg aataattctc aatcccccta tggcattgtg tcccctgctg tgctatactt
      781 gatacgcagt gctttcctgt tccagtctat agagatctaa atttgcagga agccaggtaa
      841 aggagaatat catgcagaaa ataggaagaa atgatccctg ctggtgcggc agcggacata
      901 aatataaaaa ttgccatatg gattttgacg taaaactgtc agaataccgt cacaaaggtt
      961 ccaaggtacc aagtcatgcc atgatcaaaa atccggagca gatcgcagca atccgcgaaa
     1021 gcgcaaagat caatgtgtct gttttggatt atgttgcaga acatatctgt gccggcatct
     1081 caaccgagca gatcgatctg tgggtttacg aacagactac tcacagaggc ggaattccgg
     1141 ctcccttaaa ctatgaagga tttccaaaga gcgtctgcac ctctgtaaac gatcaggtat
     1201 gccatggtat cccttctgct gatgtgatct taaaagatgg tgatatcatt aatgtagacg
     1261 tttctactat ttataaagga tactactccg actcttccag aatgttctgt atcggtgaag
     1321 tctcccctga aaagaagcgt ctggtagaga taaccaaaca ggcgatcgaa gaaggtttaa
     1381 aacaggtacg gccatgggga cacttaggtg atatgggtca ggccatcaac gactatgcta
     1441 ctgcaaacgg ttataccgtt gtccgggaga tcggcggtca cggcgtaggc ctggaattcc
     1501 acgaagatcc atgggtaagt tttgtttcta aaaaaggaac agagatgctt atggttccgg
     1561 gtatgatctt taccattgaa cctatgatca atatggggaa agcagatatc ttcgttgacg
     1621 cgaaaaatga ctggaccatc tatacagatg acggtatgcc ttccgcccag tgggagatcc
     1681 aggttctgat cacagaggat ggatacgaga tcctttcctg gtaacaggat ccggcagcca
     1741 gatctgtctc ttacaggtct ggctgccgtt ctgtaaacga tatatccgaa acattcaaaa
     1801 acttattttc tatatttaat tccagccgtc tggcagatat tttctggtgt tttcgatcat
     1861 atcatgtacc aggctgcgca cctgtgcctc ctgaggctgc tttcccttta caacagggtc
     1921 atgcaggaaa gcctgtacta ccagttcttc atcacaggta agtgctgcct ttaaggtcct
     1981 ctcatggttt tcaatgtgag gcatcagcag ggcttttaca ttttcaggta ctttccctgc
     2041 agccaccgga tggatactgt ctctgtcaaa cagggcgttt gtctctacca gggcattttc
     2101 cggaagattt gggatctgac cggctgtatt tgggatattt acattgctga ctacacgtcc
     2161 cagtccacac agtgccttga tcagaagaat tccctcttct cctgttggcg tcagatcgat
     2221 ctcctcttct cctgctgcca gtctgtgact tctctccaga cgtttcttca gatcttcttt
     2281 tctccagtct actgttgtca gaccgaactt ccaggaagca actgtttccg gatcattcag
     2341 atactcattt ccaggcataa attccgccag atgacggtct cctgctgctg cgatcagccc
     2401 atacctgcaa aaaagatcca tttttacacg attggcgcat ttaaaagaag aattcatcca
     2461 gttttcacct tccagctgat aaccttcttc aaaatgatga tctatataat tcttgtatac
     2521 agggaataag tcaactcctt tataacttgc ctccgtaaac caggtgaaat ggttgatccc
     2581 cactacattt acatgaatat catgccagtc tgcaatcttg tctcctgttt cttcttcgta
     2641 gatccccctt aaaaccttct gggtaccaaa tacctcatgg cagcagccaa atgccttgat
     2701 ctgcggaaat acatgataca gtgttttcac acacagggac ataggattgg tataattgat
     2761 cacccagcaa tcaggagcat attttttgat cgcctctgcg atctctacaa acatagggat
     2821 cgtgcgcagc cctcttatga tcccgcctgg gcctgctgta tcacctactg actgataaat
     2881 accaaggcgc tccggcagat gtacatcgga agccatctca tcaaaagttc ccggtaaaat
     2941 agagatcact gcaaaatcac agcctgtaag aacttctttc aaactgtcct ttacctcaaa
     3001 cttccagtct ccctttgcct ccggtcggtt catcaggtga ttgccgatca cttcattggt
     3061 ctgtgctgct ttcctgtcaa tgtcatataa acggatggtt ccgctgatct cgtcttccag
     3121 tgccagatcc gtcataaatg tccacgccca tcctctggaa cctcctccaa tgtatgcaat
     3181 ctgaatatca gaaacttttc ctttactgta cttcatatac gctgcctcct gttacaatat
     3241 gtgtccttta tgtaggtcct tttatgtgtc tcattttaga ataaaatatg taaaatcgct
     3301 aacaaaatca gatatatttt tgtagttttt atcttatcag ctacttttaa atgcaactct
     3361 atgctataat aggcgcagca ggaacaggag cacttatcat tatggaacag gacacacttt
     3421 atctttcttt cagtgaaata cctgacgcat ctgaaatttt atttaaaggg gaaattcccg
     3481 gcattttcgt tgaagaagcc atccgcagcc acagtttttc catggtcaca cgccattttc
     3541 atgaaagcct ggaattatat tttctcatag aaggggaacg tttttatttc gtagaacagg
     3601 atacctatca tttgcaggaa catatggcta tcctggtaga ccagaaccag atccataaaa
     3661 cctgccctgc aggtacagga aaaaagcacc accgttttct gatgcagtta gaaggacctg
     3721 tattagacca gcttctccgc ctgtgtggat ctgtaagtct ggaagaattt ggaaatgcct
     3781 atcgtggtat tacaagattt tctgtggatc aatgggctca gattctttcc cttctgacac
     3841 agatcaagga acaatttaca cttatctcat cccggacaac tcccggcaat aaacaggaca
     3901 caatgactgc caacggtttg atccgtctgt ataccgttca gcttctcctt ctcctgatcc
     3961 agatcagaag gacgcaggaa gagggcggct ggaaggaaaa ccttcaaaac aatctggtcc
     4021 acacaggtat gtatcagcag gtacatgata ttgctattta tctccaaaat cactgcagtg
     4081 agaaaaacag cctggatcag atcgctgccc attttttcat cagccgttcc tacttgaccc
     4141 ggatctttcg caaagttacc ggttttacca ttacagaata ccagaacatc tgccgcatca
     4201 aaaaagccca gcttctcctg cgagaaactg agctttccat gacggatatt gcccttgaag
     4261 cgggctttat aaatctcccc tattttgaac gagtatttcg caaaactact tctcttacac
     4321 cccttcaata ccgccgtcag atgcggccat attcataacc tgacatttca tatacctgga
     4381 ttcgggcatc acactgccct ttaagtcctg cttttccctg tttaccatac acacgggtag
     4441 taaatacctg gcttccctta ttaataaaca gttccaaaga agttgtgtcc gcaaacaaag
     4501 taaggtctgt cagttcttcg atccatacct tgcggatcgt cctgccgctt ccacagttac
     4561 ccaggtccag cgtcagaaca tgatccatat aggtcagttt tacttcatcg ctgatggaaa
     4621 attccatatt tttgcaatcc ttaaagtcga tctgcacttt ggcacatttg ggtagttcca
     4681 gattttggaa ttcttctttg gaaaatgttc tgccattccg gcaaagtttc tccaattcct
     4741 ttaccggctc ctgatacagc tttccatccc ttatatggag ctgtctcggg atcgttaagg
     4801 catgctgcca gccttttttt acagtaggat ttgtataatc tgcatccggt attcccatcc
     4861 atccgatcag gatccgtctt ccctgctgat ccgcaaaggt ctgcggagca taaaaatcaa
     4921 atccccggtc taccatctga aaattatctg tagtcttaat tacccaggtt cccggtgtaa
     4981 aatcataatc taacggaata catacacact gatggacatt ccagtaatca tatcccttct
     5041 tttctacacc ctgagggcaa cagatcagac acagctgtcc atccagttca aaaagatccg
     5101 ggcattccca catatagcca aaggcatttt ctgtggtgat ccgtccatga tatttccagt
     5161 ttgtcaggtc atcggaacgg tataccagta ccaggccctt gctctctttg tcccttgctc
     5221 ctaagaccat gtaatagcca tcttccctct taatgatctt tggatccctt acatgggcac
     5281 tgatatctgc cggataatca ctgttggtca tcaggcattt cttcggtgaa aaatgcattc
     5341 cgtcttcgct ggtgacatac atggtattgc tgactcttcc actgttgatg taatcataat
     5401 ccgggcggtc aaacaatttt acatttcctg tataaaagaa gttgatcttc ccattttcac
     5461 agaaagcaga gccggaatat acgccatgag catcctcatc gctgtccggg cataagaccg
     5521 gctggcactg tttccatagt gtacct
//
LOCUS       DBMDUA010000177         5536 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5763_length_5536_cov_2.080405, whole genome shotgun sequence.
ACCESSION   DBMDUA010000177 DBMDUA010000000
VERSION     DBMDUA010000177.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5763_length_5536_cov_2.080405"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /locus_tag="ACZPCL_11210"
     CDS             <1..1559
                     /locus_tag="ACZPCL_11210"
                     /inference="COORDINATES: protein motif:HMM:NF016497.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HIR1163068.1"
                     /translation="WRDPYVFYNEEDKCYWILISARRLDMPVTRRGCIVLYRSKDLVN
                     WEILSYALTDETKFDIKGMDTACGIWAPNLTYDNGTFYLIYTIVYTNRHRFKDTHNFM
                     VTATDPQGPWSAPIPLNKMGFDPSLFHDPDGTKWLVNMLFDYRLDHKRFGGVGVQQYD
                     PKTQKLVGPIYNVFKGSDKGTTEGPNIFYHNGWYYLVTAEGGTEFKHCVTMCRSRKLT
                     GPYEISPYAPVLTSFGKDVQLKRAGHGQIIQGKDGNWYMAHLCSRSLDDCSIVGRETA
                     IQNMTLTEDGWFKLSANDTASPEDFFEVPEEVEVKSAKSGFTDFGKVTEIPLEYMTLR
                     QSAKTCGITISDGKLHIQGGNSLASKYNQGLLVRRHQHHSYDFTAKMEFAPRDYCHMA
                     GLVCYYNYDNNYYLNMSVDDEGKPYVMVNVSVNKEISQSEKVYLGENKDPVYLKAEVR
                     ERNVTFFVSQDGTSYTQVGEVLDMRNLSDERVDGNGFAGAMVGVTCQDLHGDGIQADF
                     EWLDYSVCRE"
     gene            complement(1639..2769)
                     /locus_tag="ACZPCL_11215"
     CDS             complement(1639..2769)
                     /locus_tag="ACZPCL_11215"
                     /inference="COORDINATES: protein motif:HMM:NF023568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR1163069.1"
                     /translation="MKTVNGASLYAPAVYGPAGRILCTVYTAEGNGPHPVLIFTHGYP
                     GHEKNLDLAQSLRRTGVSSVIFFYRGCWGSEGDFSFEGSFEDTKAVLDFVLNDTQYGF
                     DKNHIFFIGHSLGCVNAARMIALCPQVKGGVFLAPCDLWRIYLLGLENEDYREHLNDL
                     LEEGIPYIHGTDEETLTNEIKGSAGRLSIGSCLQAISGKSILWISSPDDEVVSEAEET
                     YPYIKKIKHFSPSCFKWLRVSSDHYFSNMRSQLTEDIASFLLENTDHKKAHIDQASFE
                     VQLRKLICQQLAGITLPDVAAYFHISIPYASDLIRQITGSNFTALVLEERMRESEKLL
                     KNSNVPVSKVALLSGYQEPSYFMKVFKKYFGCTPSQYRDSQK"
     gene            complement(2932..3765)
                     /locus_tag="ACZPCL_11220"
     CDS             complement(2932..3765)
                     /locus_tag="ACZPCL_11220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR1163070.1"
                     /translation="MCCKNTNAKETHFGACCSRPDLYGIIIPGKHGKLLATLYTAGGE
                     GPHPTILLLHGIPGSEQNLDLAQAFRRAGFHVMTFHYSGSWGSSGNYSLQNDLDDAET
                     VLDFILNDTTYGFDKDKIYAAGHSLGGFVCGQIAARRPEIKGAVLLMPCNVGRIGKIA
                     QSDPENAKVLEDVLNDSVNWLTGLTKGCLYKEATEHEKEYALEYQAAALSKKPLLCIT
                     GSLDICTPKKDHLQPLLDGIDALGGGNIQVLEYPTDHFFSDYRLEVSDVVTEFFKDLA
                     K"
     gene            complement(3830..5101)
                     /locus_tag="ACZPCL_11225"
     CDS             complement(3830..5101)
                     /locus_tag="ACZPCL_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR1163071.1"
                     /translation="MGEKKKQASAYREPLILIAFVILGAIIGMVMGEKASFLYPIGQI
                     WLNLLFVLLVPLLFFSISSSIANISDTKSVGKLLGFTLVIFVVTAAIAGSFMFVVLGI
                     FGVDTSVQLGAGDATAQAAASSIGDQIVNTFTVGDFPDVISRGHILALIVFTVFFGVT
                     VSSLGEKGREIANWLNNMSLVFYKMVAILMKAAPLGLMAYFANLTGTYGSDLLKSYGR
                     GLLIYYPTVFVYFFVFLGLYAFIAAGPWGVKHYFKEILTPALTALGTRSSAAALPMQL
                     DACDKLGVPRVVSSVVVPVGATCHMDGACLATIYEIVLCCTLFGHPFRSIGDYAFALT
                     IAVCASVAVSSVPGGGAAMETMLISSFGFPVIALPVLMMMTQLFDAGCTLINSCGDTV
                     ASMLVSRFLYGKDWYKRDLNKELAEAEQNKE"
BASE COUNT         1694 a         1160 c         1230 g         1452 t
ORIGIN      
        1 actggcgtga tccgtatgta ttttataatg aggaagataa atgctactgg attctgatct
       61 cggcgagacg gcttgatatg ccggtgacaa gacgcggctg tattgtcctg taccgctcga
      121 aagatctggt aaactgggag atcctttcct atgctcttac agatgagact aaatttgaca
      181 taaagggaat ggacactgcc tgcggcatct gggcgccaaa ccttacttat gataatggta
      241 ctttttatct gatctatacc attgtatata ctaaccgcca tcgttttaaa gatacccaca
      301 attttatggt aactgccaca gatcctcagg gtccatggtc agctcccatc cctctcaata
      361 aaatgggctt tgatccctct ctctttcatg atcctgatgg taccaagtgg ctggtgaaca
      421 tgctttttga ttacagattg gatcacaaac gttttggcgg cgtaggtgta cagcagtacg
      481 atccaaagac ccagaagctg gtaggaccga tctataatgt atttaagggc agcgataagg
      541 gaaccacaga aggtccgaac attttctatc ataatggctg gtattatctg gtaacagcag
      601 aaggaggcac agaatttaaa cactgcgtga ccatgtgccg ttccaggaaa ctgactggtc
      661 cttatgagat cagcccttat gcgccggtcc ttacatcttt tggaaaagat gtacagttaa
      721 aacgtgccgg acatggccag attatccagg gaaaagatgg aaactggtat atggctcatc
      781 tctgctccag aagtctggat gactgcagta ttgttggccg tgaaacagcc atccagaaca
      841 tgactcttac agaagacggc tggtttaagc tgtctgcaaa tgataccgca tctccggaag
      901 atttctttga ggtgccggaa gaggtggaag taaaatctgc aaagtccgga tttacagatt
      961 ttggaaaagt tacagagatc ccgttggagt atatgacatt gcggcagagt gctaagacct
     1021 gcggcatcac catttctgac ggaaagctgc acattcaggg tggaaattcc ctggcttcca
     1081 aatataacca gggattatta gtccgcagac accagcatca ttcctatgat tttacagcga
     1141 aaatggaatt tgctccaaga gattactgtc atatggccgg acttgtatgc tattacaatt
     1201 atgacaataa ttactatctg aacatgtctg tagacgatga aggaaaacct tatgtaatgg
     1261 taaatgtttc tgtgaataaa gagatctccc agagtgaaaa agtatatctg ggagaaaata
     1321 aagatccggt atacttaaaa gcagaagtaa gagagcggaa tgtaactttt ttcgtttccc
     1381 aggacggtac ttcctacacc caggtaggag aggtacttga tatgagaaac ctttcggatg
     1441 aacgggtgga cggaaatgga tttgccggag ctatggtcgg tgtgacctgt caggatcttc
     1501 atggggatgg gatccaggca gactttgaat ggctggatta cagcgtgtgc agagagtaat
     1561 taagaacatg aaatgaaaat gtgagataaa aaacacatac cagcgggatg ggaaagtctc
     1621 tcaggtatgt gtttttgttt atttctggct gtcccggtac tggctgggag tacatccaaa
     1681 gtatttttta aacactttca taaaataaga aggttcctga tagccggaaa gcagcgctac
     1741 tttggaaacg ggaacattgc tgtttttcag cagtttttcc gattcacgca tccgttcttc
     1801 taaaaccagg gctgtaaaat tgcttcctgt gatctggcgg atcaggtcgc tggcataggg
     1861 aatgctgatg tgaaaataag cggcaacatc aggcagagtg attcctgcta actgctgaca
     1921 gatcagcttg cgcagctgga cttcaaaaga agcctggtca atatgtgctt ttttatgatc
     1981 cgtattttcc agtaaaaagc tggcaatgtc ttctgtaagc tgggaacgca tattagaaaa
     2041 ataatggtcc gaagagacac gcagccattt aaaacaggaa ggggaaaaat gttttatttt
     2101 ctttatatag ggataagttt cttctgcttc agaaactacc tcatcatccg gactgctgat
     2161 ccagagaatg gattttccag agatagcctg aagacaggag ccaatggaaa gccgtccggc
     2221 actgcctttt atttcgttgg tgagagtttc ttcatctgta ccgtggatat aaggaatccc
     2281 ttcttctaac agatcattta agtgttctct gtaatcctca ttttcaagtc caagaagata
     2341 gatacgccac agatcgcaag gggctaagaa tacaccgcct tttacctgag gacaaagggc
     2401 gatcatacgg gccgcattga cacagcctaa actgtgaccg ataaagaaaa tgtggttttt
     2461 gtcaaaacca tactgggtat catttaatac aaaatccagg acggcttttg tgtcttcaaa
     2521 acttccctca aaggaaaaat caccttcact tccccaacaa ccacgataga agaagataac
     2581 agaagagacg ccggtcctgc gtaaagactg tgccagatcc agatttttct catgtcccgg
     2641 atagccatgg gtgaagataa gaacaggatg cgggccgttt ccttccgccg tatacacggt
     2701 acacaaaata cgtcctgccg gtccatagac ggcaggtgca tataaagacg cgccatttac
     2761 tgttttcatc tgcatttccc ctttggcaaa gtatatgggt attttagcat gggagaggaa
     2821 aaatgtcaaa aagatgtcaa aaagggaaga gaagtacttc aggcgacagc aggagagcag
     2881 aaacagaaaa ttccccgctg cgtatcagcg gggaaccata aagaaatcat attattttgc
     2941 cagatcttta aaaaactctg ttacaacatc agaaacctcc aggcggtaat ctgagaagaa
     3001 gtgatccgta ggatattcaa gaacctggat atttccaccg cccagggcat cgatcccgtc
     3061 taataaaggc tgtaaatgat cttttttcgg tgtacagata tccagtgatc cggtgataca
     3121 taataacggc tttttggaaa gagcagcagc ctgatattct aaggcatatt ctttctcatg
     3181 ctcggtagcc tctttgtaaa ggcatccttt tgtaaggcct gtcagccagt ttacactgtc
     3241 atttaataca tcttccagga cttttgcgtt ttcagggtca ctttgggcga tctttccgat
     3301 ccgtcctaca ttacagggca ttaaaagaac agcacctttg atctccggtc ttcttgctgc
     3361 gatctgtccg cagacaaaac cgcccaggct gtggcctgct gcatagatct tatctttatc
     3421 aaagccataa gttgtgtcgt ttaaaataaa atcaagaact gtttcagcgt catccagatc
     3481 attttgcagg gaataatttc cgctgcttcc ccaactgccg ctgtagtgga atgtcattac
     3541 gtgaaagccg gcccgtctaa atgcctgtgc aagatccagg ttctgttcac ttccgggaat
     3601 accatggaga aggagtatag taggatgggg accttcaccc cctgccgtgt ataaggtagc
     3661 aaggagcttt ccatgttttc cgggaattat gataccataa agatcgggcc ggctacaaca
     3721 ggcgccgaaa tgagtttctt tcgcattcgt atttttacaa cacatataaa cggtcctttc
     3781 tgacgtaatt catgtcttta aaagaacaga cctaaaattt gttcttatat cattctttgt
     3841 tctgttctgc ttcagcaagt tctttgttta agtctctctt ataccagtct tttccgtaca
     3901 ggaaacggga aaccagcata gaagcaactg tatcgccgca ggagttgatc agagtacagc
     3961 ctgcatcaaa cagctgtgtc atcatcatca gtaccggaag ggcgataaca gggaaaccaa
     4021 aggaagagat cagcatggtc tccatagcag caccgccgcc gggaacagaa gatacagcaa
     4081 cagaagcaca tacagcaatg gtcagtgcaa aggcataatc accaatgctt ctgaatggat
     4141 gtccaaataa ggtgcagcaa agtacgatct cgtaaatagt agcaaggcag gcaccatcca
     4201 tatggcaggt agcaccaaca ggtacaacaa cggaacttac aactcttgga acacctaatt
     4261 tatcgcaggc atccagctgc atcggaaggg cagcagcaga agaacgggta ccaagtgcag
     4321 tcagtgcagg tgttaagatt tctttgaaat aatgttttac gccccatgga ccggcagcga
     4381 taaatgcata aagtccaaga aatacaaaga aataaacaaa aacagtagga taataaatta
     4441 aaagtccacg tccatagctt tttaacagat cggaaccata tgtaccggtc aggtttgcga
     4501 aataagccat cagacctaaa ggagcagctt tcattaagat tgctaccatt ttgtagaata
     4561 ccagggacat gttgtttaac cagtttgcaa tttcacggcc tttttcacca agggaagaaa
     4621 cggtgacacc aaagaataca gtaaatacga tcagtgccag gatatgtcct ctggaaatga
     4681 catccgggaa gtcaccaact gtaaaagtat taacgatctg gtcaccgata ctggaagcag
     4741 ctgcctgtgc agtagcatca ccagcgccta actgaacgga agtatcgaca ccaaagatgc
     4801 ccaatactac aaacataaaa cttccggcaa tggctgcagt cactacgaag atcaccagtg
     4861 taaatcccag cagctttcca acgctctttg tatcgctgat attagcgata gaactggaaa
     4921 ttgagaagaa aagcagcgga accagcagta caaacagcag gttcaaccag atctggccaa
     4981 ttggatacag gaatgacgct ttttctccca tcaccattcc gatgatggcg cctaaaataa
     5041 caaaggcaat aaggatcagt ggttcacggt aggcagaagc ctgtttcttt ttttcaccca
     5101 tagataattc cctccttaga atatttatgt tacacacact ataagagttt tcagacaact
     5161 tttcaatcaa caattatagc aattgaactg taatatttta agatataatg tgtccaaaag
     5221 aaaaataagc gacaccgaag gtggcattta tttttcttgg accattaacg aacaaatcgt
     5281 ctgtgtaagc agacagtaga gcgcaaagtg cggaatttgt gagtgcagtt tgaaatttta
     5341 atgataatat cagaattttc tgaatgtttg aaatcctatt gacttgaagt caacttcaag
     5401 ctgtatactt aaaccagata cagactttat gtataaaaaa tgtatagtaa aaaacgtata
     5461 tataatagga ggaaacactt catgagcaat gtaaaaccag tacctggaac aaatggaaac
     5521 cagtatatcc cttacg
//
LOCUS       DBMDUA010000178         5509 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5796_length_5509_cov_3.330067, whole genome shotgun sequence.
ACCESSION   DBMDUA010000178 DBMDUA010000000
VERSION     DBMDUA010000178.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5796_length_5509_cov_3.330067"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            262..1344
                     /locus_tag="ACZPCL_11230"
     CDS             262..1344
                     /locus_tag="ACZPCL_11230"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR1163072.1"
                     /translation="MKITNEKQQYYGLDLLKFIMAVLVAARHVIQIYYPAESKWRLVI
                     GCWLSNLAVPIFFLIAGFLLFQKIPAQLPNDTNTTDTDIKNSWPIARRYICRILKLYI
                     LWCMIYWPIDIYNWVQGNETIAQAVLFYLHSFFMSATITQLWYLPALAIASFIVWLFY
                     RTKAKTWMLLLFTGILFILGCICDNRVLLEHTPETLQAILQAFVRWYTPRFMTMRNGV
                     FYGSFYVALGAWSAKRERHLSPVPAALGSLFFLSVMYIEVSLCSNANMVLSAAPAVVC
                     LTELALNLTGTGSRFFLFLREQSEWIYFSHYYFIHLFTWTIHYNPLPMTKTNIMLSVL
                     VPMFLFTLLISFLSHRKHGKWLRALI"
     gene            complement(1463..4825)
                     /locus_tag="ACZPCL_11235"
     CDS             complement(1463..4825)
                     /locus_tag="ACZPCL_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR1163073.1"
                     /translation="MVSAVLFITFFVLLVLNVPISICLGLSSAAAILYSGTSLTIVAT
                     NIYSGISKFLLLAIPFFVLSGNIMAKAGISTRLIRFVDTCVGHRKGGIANVCIIVACF
                     FGAISGSGPATVAALGAVLIPAMVERGGFSAPFSTALMATASSIAIVIPPSIAFVVYA
                     SITGVSIGDMFMAGIVPGILMGLALIIVVMLEVRKNHVVPSNVQKASAKERLDAFKDA
                     FWGFLMPVIILGGIYGGIFTPTEAAAVSVVYGLFVGMVIYKEVKLKDLWDILIDSAKT
                     TGGIMLIVACASLFSFVCTRFGISAAASQLLAKVAGNQFTFLLICNVIFLIAGCFIDA
                     NSAMYIFIPIMLPVCKQLGYNVIAFGIMATVNLAIGQVTPPVGVNLFVAIGVKLKNGL
                     QVTLQQISRAVVPMVAACIVVLLMITYIPGISTFLPAALGSAAAVENGTTTGGDSGTT
                     ATDNDDFNTIEDYSDLDWPEMTWNFTCSTTETSTWAQAGRKFGELMEQATGGKIKVEV
                     YAADQLTGGNQSEGIQALMNGDPVQISMHSNLIYSAFDPRFNVVSLPYLFDSVEAADA
                     VLDGPAGEELVSILESYDLHCMGMAENGFRQLTNSVHPVHSVEDMKNLKLRVAGSNLL
                     MKCYELWGADATNMNWSETYTALQQKTVDGQENPLPAIDAASVQEVQKYVSLWNANYD
                     CLFFCINQGIYDSLTQEQQKVVDEAGRKAVAYEREINRAGDEEILDRWQSKNGVEVVK
                     YEDLDVESFKNAAEPVTEWFINELKNQGYEDAEDLVHIFTDNAAKAAGAESTGSTSAY
                     QVEDHSDLNWPEMTWNFTCSTTETSTWAQAGRKFGELMDQATGGKVKVEVYAADQLTG
                     GNQSEGIQALMNGDPVQISMHSNLIYSAFDPRFNVVSLPYLFDSVEDADAKLDGEAGQ
                     KMDEILSSYGLHCMGMAENGFRQLTNSVRPVHSVEDMKNLKLRVAGSNLLMKCYELWG
                     ADATNMNWSETYTALQQGTVDGQENPLPAIDAASVQEVQKYVSLWNANYDCLFFCINQ
                     DIYDNLTPEQQAVVDECGKLAVQYEREINRAGDDEILSRWQSKNGVEVVDNDELDIDS
                     FKAIVEPVTEWYIGELQRQGYDDAEELVAAFK"
     gene            complement(4826..5344)
                     /locus_tag="ACZPCL_11240"
     CDS             complement(4826..5344)
                     /locus_tag="ACZPCL_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR1163074.1"
                     /translation="MKGYKKLMDFVALVEKAVLAATMIMILVLTVGNVFSRKVIHQSW
                     SFTEELVVAIFVLITLLGAALSAREGGLVGLNLVTERLPKSCKKPVIILITLFSVAFM
                     GILFYYGMDKVLAQLANQKRTFVLNWPEWIFWSFVPIGSVCMVLHFIEYCVDECAKTN
                     AAEKNDMKKEDK"
BASE COUNT         1431 a         1328 c         1149 g         1601 t
ORIGIN      
        1 aacggttatt tattataacc ggttgttttt catttgtcaa caactttttt aatttatttt
       61 tttgtttctt tcgaaacatt tgctgctctc tcaagcgcga aaattataat agcaaatccc
      121 caaccgtttg tcaacaactt ttttcaggtt tttacaattt tattttgcag acatgatttg
      181 tgttttttgt ttccctacaa attatgttat aatcaaatac gaaactttgc gccttccttt
      241 aacggtagaa tggagatctc catgaagatt acaaatgaaa aacaacaata ctatggactg
      301 gatcttttaa agtttattat ggcagttctg gtagctgcca gacatgtgat ccagatctat
      361 tatcccgctg aaagcaaatg gcgtctggtg atcggatgct ggctgtccaa tctggcggtt
      421 cccattttct ttctgatcgc cggatttctt ctgtttcaga agatacccgc tcaactgcct
      481 aatgatacaa acacaactga taccgatata aaaaacagtt ggcccattgc caggcgttat
      541 atatgccgca tcctgaaact gtatatcctc tggtgtatga tctactggcc tattgatatt
      601 tacaactggg tccagggaaa tgagaccatt gcccaagcag tcctttttta tctgcactct
      661 ttctttatgt cagctaccat tacccagctg tggtacctgc cagctctggc catagccagt
      721 tttatcgtct ggctgtttta cagaacgaaa gccaaaacct ggatgctgct tctttttaca
      781 gggatccttt ttattttggg atgtatctgt gacaaccgtg ttcttttaga gcatacgcct
      841 gaaacacttc aggcaatact tcaagctttt gtccggtggt acacgccccg gttcatgact
      901 atgcgaaacg gcgtttttta tggaagcttt tatgtagctc ttggtgcctg gtctgcaaag
      961 agggaacggc atctgtcacc tgtaccggcc gctttgggaa gccttttctt tctaagtgta
     1021 atgtacatag aagtttccct ctgctccaat gccaatatgg tattatcagc agcacctgcc
     1081 gtcgtctgcc ttacagaact ggcattaaac cttaccggaa ctggttccag atttttcctt
     1141 tttttacggg aacaaagtga atggatctat ttctcccatt actatttcat ccatttgttt
     1201 acctggacca ttcattataa tccacttcct atgactaaaa caaacattat gctttccgtt
     1261 ctggtcccca tgttcctgtt tacattgctt atatcatttc tgtcccacag gaagcatggc
     1321 aaatggctga gggcactcat ttagatcagc ggatgcgcca ctggcgtctc ctcgatatgc
     1381 ctggcaacaa ttaaggctga tgcaaaaaag atcctatctc ttctgcatca gccttttcct
     1441 gactttattt acttaattct acttacttaa atgctgctac cagctcttct gcatcatcat
     1501 atccctgtct ctgcagttcg ccgatatacc attcggttac aggttctacg attgccttaa
     1561 aggagtcgat atccagttca tcattatcaa ccacttctac gccattcttg gactgccacc
     1621 tggataagat ctcgtcatct ccggcgcggt tgatctcacg ctcatactga acagctaatt
     1681 ttccgcactc atctactact gcctgctgct caggagttaa attgtcgtag atatcctgat
     1741 taatgcagaa gaacagacag tcatagtttg cattccagag agatacatat ttctgtactt
     1801 cctgtacact ggctgcatcg attgctggaa gcgggttttc ctggccatct acggttccct
     1861 gctgaagagc ggtgtatgtc tcggaccagt tcatattggt ggcatctgcg ccccaaagct
     1921 catagcactt catcagaagg ttagaacctg ctactcgcag cttcaaattc ttcatatctt
     1981 ctacagaatg taccggacgg acactgttgg tcagctggcg gaagccattt tctgccattc
     2041 ccatacaatg taagccatag gaagataaga tctcatccat tttctgtcct gcttccccgt
     2101 ccagttttgc gtctgcatct tctacagagt cgaaaagata tggcaatgaa actacgttaa
     2161 aacgtggatc aaaggcagaa tagatcaggt tgctgtgcat ggagatctgt accgggtcac
     2221 cgttcatcag ggcctggatg ccttcggact ggttaccgcc ggtcagctgg tctgctgcat
     2281 atacttctac ttttactttt ccgccggttg cctgatccat taactcgccg aatttacgtc
     2341 cggcctgggc ccaggtactg gtttctgtgg tagaacaggt gaaattccag gtcatttccg
     2401 gccagtttaa gtcactgtgg tcttctacct ggtatgcaga tgtacttccg gtgctttctg
     2461 cacctgctgc ttttgctgca ttatcggtga aaatgtgtac caggtcttct gcatcttcat
     2521 atccctggtt ctttaactca ttgatgaacc attcggttac aggctctgct gcattcttaa
     2581 aggactcaac atccagatcc tcgtatttta caacttctac gccattcttc gactgccagc
     2641 ggtctaagat ctcctcgtca ccggcgcggt tgatctcacg ctcataagca actgccttgc
     2701 ggcctgcttc gtcaactacc ttctgctgct cctgggtcag actgtcataa atgccctgat
     2761 tgatacagaa gaacaggcag tcataatttg cattccagag agatacatac ttctgtactt
     2821 cctgtacgct ggctgcgtcg atcgctggaa gtgggttttc ctgtccgtct actgtcttct
     2881 gctgaagagc agtgtaggtc tcagaccagt tcatattggt ggcatctgcg ccccaaagtt
     2941 cataacactt cattaaaagg ttggaacctg ctacacgaag cttcaggttc ttcatatctt
     3001 ctacagaatg taccgggtga acgctgttgg tcagctggcg gaaaccattt tctgccattc
     3061 ccatacaatg caggtcatag ctttccagga tacttaccag ttcttctcct gcaggtccgt
     3121 ctaaaacagc atccgctgct tctacagagt caaacagata tggcagtgaa actacgttaa
     3181 aacgtgggtc aaatgctgag tagatcaggt tgctgtgcat ggaaatctgt accgggtcgc
     3241 cgttcatcag tgcctggata ccttcggact ggttgccacc ggtcagctgg tccgctgcat
     3301 atacttctac tttgatcttg ccgccagttg cctgttccat taattcacca aacttacgcc
     3361 cggcctgggc ccaggtactg gtctctgtgg tagagcaggt aaagttccat gtcatttccg
     3421 gccaatccag atcactgtaa tcttcaatgg tattaaaatc gtcattatca gttgctgtgg
     3481 taccgctgtc accgcctgtg gtcgttccat tttcaaccgc tgccgcactt cctaatgctg
     3541 ctggaaggaa agtggaaatt cccgggatat aggtgatcat aagcagaacc acgatacatg
     3601 cagctaccat aggtacaact gcacgggaga tctgctgtaa agttacctgt aaaccattct
     3661 tcagctttac accgatggct acaaacaggt tgacaccaac tggcggtgtt acctgaccga
     3721 ttgccaggtt gacggttgcc ataataccaa aggcgatcac attgtagccc aactgcttgc
     3781 atacaggaag catgatcggg atgaaaatgt acatggctga gtttgcgtca ataaagcagc
     3841 ctgcgatcag gaagatcaca ttgcagatca gcaagaaagt gaactgattg cctgctactt
     3901 ttgccagcag ctgggaagct gcagcagaga taccaaaacg ggtacataca aaggagaaca
     3961 gggacgcaca tgctacaata agcatgatgc cgccggtagt ctttgcagag tcaataagga
     4021 tatcccaaag gtcttttaat tttacttctt tataaattac catacctaca aaaaggccat
     4081 atacaacaga aacggctgct gcctcagtag gtgtgaaaat accgccgtaa ataccgccaa
     4141 ggatgataac tggcattaaa aatccccaga atgcgtcttt aaatgcgtcc agacgttctt
     4201 ttgcagatgc cttctgtaca ttggacggaa cgacatggtt tttacgcact tctaacatta
     4261 ccactatgat cagtgcaagt cccatgagaa ttccaggaac aataccagcc atgaacatgt
     4321 caccaataga aacgccggta atggaagcat acacaacgaa ggcgatactt ggcgggatga
     4381 caatggcaat ggagctggca gttgccatta atgcagtaga gaatggagcg gaaaagccgc
     4441 ctctttctac catagccggg atcagaactg cgcctaatgc tgctaccgtt gcaggaccgg
     4501 aaccggagat cgcgccgaag aagcaggcaa cgatgataca tacatttgca atgccgcctt
     4561 tgcggtgtcc aacacaagtg tcaacgaaac ggatcagtct ggtggaaata ccggcctttg
     4621 ccataatgtt tccggataat acgaaaaacg ggatagcaag aagcaggaat ttgctgatac
     4681 cggagtaaat gttagttgct acaatggtta aagaagttcc tgaatacaga atcgctgctg
     4741 cggatgaaag tcccaggcag atagagatcg gaacatttaa aaccagtaaa acgaaaaagg
     4801 tgataaaaag aactgcactt accatttatt tatcctcctt tttcatatca ttcttttctg
     4861 ctgcgtttgt ttttgcacat tcatccacac aatactcaat aaagtgcagg accatgcaca
     4921 cggaaccaat ggggacgaag gaccagaaga tccattccgg ccagtttaat acgaaggttc
     4981 gtttctggtt ggcaagctgt gccaatacct tgtccatacc gtaatagaat aaaattccca
     5041 taaaagccac ggaaaaaagt gtgatgagaa tgatcactgg ctttttacat gactttggca
     5101 ggcgctctgt taccaagttt aaacctacca gacctccctc tcttgcagac aatgctgcgc
     5161 ctaaaagggt gatcagaaca aaaattgcaa ccaccagttc ctctgtaaaa gaccatgact
     5221 ggtggatcac tttacgtgaa aacacgtttc ctactgtcag tacaaggatc atgatcatgg
     5281 tagctgccag aactgctttt tcaaccagcg ctacaaagtc cataagcttt ttgtaccctt
     5341 tcatactgct ctcctcctaa atatgtaatg cttttacctg tgtaagatcc tgtgttattt
     5401 ccgctttttc tatatcattc ttttgcatga tgcatcagtg aatataaaaa atccgcccct
     5461 taagatctaa gggacggatg attaactgat cctgtcagaa gttttcact
//
LOCUS       DBMDUA010000179         5489 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5817_length_5489_cov_1.576281, whole genome shotgun sequence.
ACCESSION   DBMDUA010000179 DBMDUA010000000
VERSION     DBMDUA010000179.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5817_length_5489_cov_1.576281"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="ACZPCL_11245"
     CDS             complement(<1..413)
                     /locus_tag="ACZPCL_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HIR1163075.1"
                     /translation="MADVLSQSQIDALLKSMSADAPAQETKTESVKPQPPEEKNYIKY
                     DFFSPRKFTKEKMKILRSVFENYARILTSQINGIFRVMTDITVLDVQERRYYEYVSAL
                     HENDCMTMIDASIAGEEKMSVPLMLFVTPGMAITL"
     gene            complement(429..1367)
                     /locus_tag="ACZPCL_11250"
     CDS             complement(429..1367)
                     /locus_tag="ACZPCL_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA/MotB family protein"
                     /protein_id="HIR1163076.1"
                     /translation="MSKKNRPTDSGGSWMDTYGDMVTLLLTFFIMLYSMSNLDAEKWT
                     IFVKSIPTMQNQESEHITINSQMSDTPNADSIGQKISQESAQEDRQIEDINKLYLTIA
                     ESLNQAGVKDATVIRGDDYTYVSFANNVFFGANSSVLTREGQLVLHTFAKAIAPAAGG
                     IEQVNIMSHTAKVTDNSENNPQTVRKDRILSAMRSAEVCIYLQKQNVIKPEKLVDISY
                     GEYRPIADNSTEEGRIKNRRIEFLLLDNQAKERDLDEYYKDFKSGEYSNTTVVTVGQS
                     QGSSQDGETVPADISAADEEITSDTGNSAGTQNTSE"
     gene            complement(1364..2182)
                     /locus_tag="ACZPCL_11255"
     CDS             complement(1364..2182)
                     /locus_tag="ACZPCL_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009534812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HIR1163077.1"
                     /translation="MDPTTLLGIILGFALIVNGIGFDKIGNFINVPSMVIVIGGALSA
                     TLSSYPLGILKDIPKHFAVLVRGKRYDIPKLVEQLVDLGTIARQSGLLALEDKAAEIK
                     DPFFKQSVLMIVDANDPDKVRLVLERELDNMMVRHDQAAGLYEKAASYAPSFGMIGTL
                     IGLINMLKGMNMDAGGSSSIGSDMSVALITTFYGCILANVIFNPIAKKLRIRQDEEEL
                     YCSTIIEGIIAIQAGENPKYLREHLLASIKQSQQQKILEKAETGDSQGKEQEDK"
     gene            complement(2195..2398)
                     /locus_tag="ACZPCL_11260"
     CDS             complement(2195..2398)
                     /locus_tag="ACZPCL_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HIR1163078.1"
                     /translation="MICVERLNGEILYLNWLQVELIEQIPETKIRMMNGDYYLVKDSV
                     ESIKDQVTGFFQRCVSIKKEKQI"
     gene            complement(2404..3324)
                     /locus_tag="ACZPCL_11265"
     CDS             complement(2404..3324)
                     /locus_tag="ACZPCL_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HIR1163079.1"
                     /translation="MLRAMDSAVAGLRAHQNKLDVIGNNIANVNTFGFKSQSYSFKEA
                     MYQTSTTSTKGSENAGGVNAAQYGYGSMMGSISTDMSASTPTYVGGLNASVNGEGFFI
                     TFPKSTGNVAVADMKKTDFDYTRVGQFKIDGNGYLTDANGNFVYGFRPDAVEAPTKFD
                     GELVAIKVPSEVTGNNFGGDPLQLTNVKINSLGEISGTVKSTDKDKDGKTVSIGKVAI
                     ATFQNPEGLSKKGSFYYGAAESDNSGNASATIPGGSTSSLMTGYLEASNVDLAKEFSD
                     MITTQRGFQANSKIITVSDEILSDLINMKR"
     gene            complement(3341..3937)
                     /locus_tag="ACZPCL_11270"
     CDS             complement(3341..3937)
                     /locus_tag="ACZPCL_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HIR1163080.1"
                     /translation="MADEITVSSTANPYQTGVYSAGSNSKNTMDVNSFIKLLAAQLSN
                     QDMTSPMDNSEMMSQMTNMAMVQSLTAMTESLNTSAAVTTQTYAGSLIGQEVTVAVTD
                     PDTGTANGVKYGKVVSVDFTKGDPVVRLEGDDTGYKMSSVLGVGKLQDTQDKTDKSEA
                     AGEKDPDRGSEKTDKITGQLANADLKDTGSEDTETEEM"
     gene            complement(3956..5146)
                     /locus_tag="ACZPCL_11275"
     CDS             complement(3956..5146)
                     /locus_tag="ACZPCL_11275"
                     /inference="COORDINATES: protein motif:HMM:NF014207.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HIR1163081.1"
                     /translation="MNRIENTGQIKTGYSVKSGTNPEEKVENGSDFASMLTKYKKKAA
                     QDDETKRAETGETKNKETQPEETKSGENGKQVSGSQSGKNKKTAEIKAEIKVTDIQDG
                     KNSQDGEIEQDQELAAAYASETCYRSFVQITTENNKMPAVYNNEIQDLAVQITAEAGM
                     EDQSAAVSTIQKNEAPHTETVAHADDLKLQNRLAQTEAAGRTGEAGQSKQTAKMNREE
                     EIPDQKEHISGKEMTALASSSYTGTVESSAGTAAKPAAVDAGPADRLATAQDTLTEDL
                     GNYLDTKISEKKGKLELSLEPERLGKLTIRLEYEKGKTEVTIFSTSAKTLEILSKEAG
                     HLAQILEEKTGTPTVIYTPEQTENRQNMDQDTGHGRNGRQDQKEQRQKQDDSFAQQLR
                     LGLA"
     gene            complement(5148..>5489)
                     /locus_tag="ACZPCL_11280"
     CDS             complement(5148..>5489)
                     /locus_tag="ACZPCL_11280"
                     /inference="COORDINATES: protein motif:HMM:NF014144.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HIR1163082.1"
                     /translation="IQELENKLLSVQDKMDDLKKQDVQIKDICLYGMYISEMEDQIRK
                     KQEQLKLLQQQEEKKKEQVIAAKIDTSRYEKLKDRRQSEYEKAAKKAQELFIEEYASR
                     TARYSQNREVI"
BASE COUNT         1208 a         1366 c          984 g         1931 t
ORIGIN      
        1 atcagcgtga tcgccatacc tggagttaca aacagcataa gtggaacact cattttttcc
       61 tctcccgcta ttgatgcatc tatcattgtc atgcagtcat tttcatgaag agcacttaca
      121 tattcgtaat atcgtctttc ctgtacatcc agcaccgtta tatccgtcat cacacggaat
      181 ataccgttga tctgggaagt aagaatgcgc gcataatttt caaatacgct cctgaggatc
      241 ttcatttttt cttttgtaaa tttcctcggg ctgaaaaaat cgtattttat gtagtttttt
      301 tcttccggcg gctgaggctt tacggattct gtttttgttt cctgcgctgg tgcatctgca
      361 ctcatagatt ttaaaagtgc atctatctgg ctttgtgata aaacatctgc cattcttcct
      421 aacctctctt attcactggt attttgtgtt cccgccgaat ttcctgtatc tgacgttatt
      481 tcttcatctg ctgcactgat atctgccggt acagtttcac catcctggga agagccttgt
      541 gactgtccta ctgttaccac tgtagtgtta ctatattcac cgctcttaaa atctttataa
      601 tattcatcca gatctctttc ttttgcctga ttatctaaaa gcaaaaactc gatcctgcgg
      661 ttttttatcc ttccctcttc tgtggaatta tctgctatag ggcgatactc accatagctt
      721 atatccacca gcttttcagg ttttattaca ttctgcttct gaagataaat acatacttct
      781 gcagatctca tcgccgaaag gatacggtct tttcgtacag tctgtggatt gttctctgaa
      841 ttatctgtta ctttcgcagt gtgggacatg atattaacct gttcaatccc acctgctgca
      901 ggagctatgg cttttgcaaa tgtatggagg actaactgtc cttcccgtgt aagcacggag
      961 ctgtttgcac cgaaaaacac attatttgca aaagaaacat acgtataatc atctcccctg
     1021 atgaccgtag catcctttac acccgcctga ttcaggcttt cagcgatcgt aagatacagc
     1081 ttatttatat cttctatctg ccgatcttcc tgggcgctct cctgactgat cttctgacct
     1141 atgctatctg catttggtgt atctgacatc tgggaattga tagtaatatg ctctgattcc
     1201 tggttttgca ttgttggaat acttttcaca aaaatagtcc atttctctgc atccaggtta
     1261 gacatggaat acagcatgat aaaaaaggta agcagcaatg ttaccatgtc tccataagta
     1321 tccatccagc tgccaccact gtctgtggga cgatttttct tactcattta tcttcctgtt
     1381 ccttcccctg agaatctcca gtttctgctt tttcaaggat cttctgctgc tgtgactgtt
     1441 tgatcgatgc cagaagatgt tccctcaggt attttggatt ttctcctgcc tgtatggcaa
     1501 tgatcccttc aatgatagta gaacaataca gttcttcctc atcctgacgg atcctcaatt
     1561 tctttgcgat cggattaaaa ataacatttg caagaataca tccataaaag gttgtgatca
     1621 atgctactga catgtcgctt ccgatacttg aagaaccacc tgcatccata ttcattcctt
     1681 tcagcatgtt gatcagacca atcagggtac ctatcatacc aaaagacggt gcataagatg
     1741 ccgctttttc ataaagtcct gctgcctggt catggcggac catcatatta tccagttctc
     1801 tttccagaac cagccttacc ttgtccggat cgtttgcatc tacgatcatc aggacactct
     1861 gcttaaagaa aggatccttg atctccgccg ccttatcctc cagcgccaga agaccgctct
     1921 gccttgctat cgttcccaga tctacaagct gttctaccag cttcggaata tcatagcgtt
     1981 ttcctctcac aagtactgca aagtgctttg gaatatcttt taagatccct aatggataac
     2041 tggacaaagt tgctgacagc gcaccaccaa taacaatgac catactggga acattaataa
     2101 aattgccaat cttgtcaaaa ccgattccgt ttacaattaa agcaaaaccc aggattatac
     2161 ctagtaatgt tgtaggatcc acctgctttt cctcctaaat ctgcttctct ttttttatgc
     2221 tgacacacct ttgaaagaac ccggtcacct gatctttgat actttctaca ctgtctttta
     2281 ccagatagta atctccattc atcatacgga tctttgtttc cgggatctgt tcgatcaatt
     2341 caacctgaag ccagttcaga tacaaaattt ctccgttaag tcgttctaca cagatcatat
     2401 tacttaccgc ttcatgttga tcaggtcaga caggatctca tcactgactg tgatgatctt
     2461 tgaattggcc tgaaagcctc tctgtgttgt gatcatatca gaaaattcct ttgcaagatc
     2521 cacattggaa gcctccagat atccggtcat taaagaagag gtgcttcctc ctgggatcgt
     2581 agcggaagca ttaccactgt tatcgctttc tgctgcccca tagtaaaaag aacccttttt
     2641 gcttaagccc tccggattct ggaaggtggc aatagcaacc ttaccgatgc tgacggtttt
     2701 tccatcctta tccttgtccg ttgactttac tgtacctgaa atctctccca gactgttgat
     2761 ctttacattt gtcagctgca gcggatcacc accaaagtta ttgcctgtta cctcactagg
     2821 aaccttgata gctacaagtt ctccgtcaaa ttttgttgga gcttctaccg catccggacg
     2881 gaaaccataa acaaaatttc cgttggcatc tgtaagatat ccatttccgt caatcttgaa
     2941 ctgacccact ctggtataat caaaatccgt tttcttcata tccgctactg caacatttcc
     3001 tgtgctcttt ggaaatgtaa taaagaagcc ttctccgttt acgcttgcat taaggccacc
     3061 tacataagtt ggtgtagagg cactcatatc tgtactgatg gaacccatca tggaaccata
     3121 tccatactgg gctgcattta caccaccggc attctcgctt ccctttgtgg aagtggttga
     3181 agtctggtac attgcctctt taaagctgta actctgggac ttaaaaccaa atgtatttac
     3241 gtttgcaata ttgttgccga tgacatccag tttgttctga tgtgcccgaa ggcctgctac
     3301 tgctgaatcc attgctctta acatattctt ttgctccttt ttacatttct tctgtttctg
     3361 tgtcttcaga tcctgtgtct ttcagatctg catttgctaa ctgtcctgtg atcttatctg
     3421 ttttttcaga tcccctgtca ggatcttttt cacctgctgc ctctgactta tctgttttat
     3481 cctgtgtgtc ctgaagcttt cctactccca ggacactgga catcttataa ccagtatcat
     3541 caccttccag acgtactact ggatctccct tggtaaaatc aacagaaact acctttccgt
     3601 atttcacgcc atttgctgtc ccggtatcag gatctgtaac tgccacagtt acctcctgac
     3661 cgatcaggct gcctgcatac gtctgtgttg taacagcagc tgatgtattt aatgactctg
     3721 tcatggcagt aagtgactgg accattgcca tatttgtcat ctgggacatc atttcgctgt
     3781 tatccatagg gcttgtcata tcctgattgg aaagctgtgc tgctaacagc ttgataaagc
     3841 tgtttacatc catcgtgttc ttgctgttgg atccggcact gtaaacacct gtctgatacg
     3901 ggtttgcagt gctgctgact gttatctcat ctgccatatc actttaatcc tccttctagg
     3961 ccagtcccag ccttagctgt tgggcaaagc tgtcatcctg cttctggcgc tgctcttttt
     4021 gatcctgcct gccgtttctg ccatgaccgg tatcctgatc catgttttgc ctgttttcag
     4081 tctgttccgg tgtgtaaata acagtaggag ttcctgtttt ttcttccagg atctgcgcca
     4141 gatgccctgc ttctttgctt aatatctcca gtgtttttgc actggttgaa aaaatagtga
     4201 cttctgtttt tcctttttca tactccagcc ggatagtaag ctttccaagc ctttccggct
     4261 caagggaaag ctccagcttt cctttttttt cactgatctt tgtatccagg tagtttccca
     4321 gatcctctgt caatgtatcc tgggctgttg ccagccggtc tgccggtcct gcatcaacag
     4381 cggctggttt tgctgctgtt ccagctgaac tttcaacagt tcctgtataa ctggacgatg
     4441 ccagggctgt catttccttt ccggaaatat gttccttctg gtctggtatt tcttcttccc
     4501 ggttcatttt ggcagtttgc tttgactgcc cggcttctcc ggtccgtcct gcagcttctg
     4561 tctgtgccag tctgttctgg agctttaagt catctgcatg agcaactgtt tctgtgtggg
     4621 gggcttcgtt cttctgtatg gtactgacag ctgccgactg atcctccatt cctgcttccg
     4681 ctgttatctg tacagccaga tcttgtattt cattgttgta aacagcaggc attttattat
     4741 tttctgttgt tatctgtaca aagctcctgt agcaagtttc agaagcatag gcagctgcca
     4801 attcctgatc ctgctctatt tctccatcct ggctgttctt tccgtcctgt atatccgtta
     4861 cttttatttc tgcttttatt tcagctgttt ttttattctt tccggactga ctgccactaa
     4921 cctgttttcc attttcccca cttttggttt cttctggctg tgtttcttta tttttagttt
     4981 cccctgtttc tgctcttttt gtttcgtcat cctgggctgc ttttttcttg tattttgtca
     5041 gcatcgatgc aaaatctgac ccattctcca ctttttcttc tgggttcgta cctgacttta
     5101 cagaatatcc tgtttttatc tgaccagtat tttcaatcct gttcaaccta gattacctcc
     5161 ctgttttgag aatatctggc tgttcttgaa gcatattctt ctataaacag ctcctgtgcc
     5221 ttttttgctg ctttttcata ctcactctgc cgcctgtcct ttaacttttc ataacgggac
     5281 gtatctatct ttgcagcgat cacttgttct tttttctttt cttcctgctg ctgtaaaagc
     5341 tttaactgtt cctgtttttt ccggatctga tcttccattt cactgatata cataccataa
     5401 agacaaatat cttttatctg tacatcctgt tttttcagat catccatctt atcctggaca
     5461 gataacagct tgttctccag ttcctgtat
//
LOCUS       DBMDUA010000180         5477 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5833_length_5477_cov_2.498884, whole genome shotgun sequence.
ACCESSION   DBMDUA010000180 DBMDUA010000000
VERSION     DBMDUA010000180.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5833_length_5477_cov_2.498884"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="ACZPCL_11285"
     CDS             <1..536
                     /locus_tag="ACZPCL_11285"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR1163083.1"
                     /translation="NGKFLSLGILFVPALFFITLGTDNGVNFTDGLDGLCTSVTILVA
                     TFLTIVAIGENMGISPITGAVVGSLLGFLLFNVYPAKVFMGDTGSLALGGFVASAAYM
                     MRLPLFIPIIGLIYLVEVLSVIVQVTYFKKTGGKRIFKMAPIHHHFELCGWSETRVVA
                     VFSIVTALLCMIAYLGL"
     gene            599..2155
                     /locus_tag="ACZPCL_11290"
     CDS             599..2155
                     /locus_tag="ACZPCL_11290"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009533991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR1163084.1"
                     /translation="MNQSKVLVAGTGISGIAAAKLVLERGGEVVLYDGNTALNTDEIR
                     AKFGKDAKVGIVLGEIKRSDLLGVELCIISPGIPLNSGFVAVVDEAGIPVLGEIELAY
                     QCGLGKLAAITGTNGKTTTTALTGAILRSQYEETFVVGNIGEPYTSKVSEMTDQSVTV
                     AEVSSFQLETIMDFHPDVSAILNITPDHLDRHGSMENYIRIKECITMNQTAKDAVVLN
                     YDDPVLREFGESRIEEPEPAEDAADTETEAVAREDGLDGAEDLREPDETAGEEPEQSE
                     TEVTETVSESEAEETDPEEVLSGLKARVFWFSSRERLKEGFFLDGDNIVYADGTKEQI
                     LVNVKELQLLGRHNYENVMAAAYIGLLMGVPFEKIRETVRKFKPVEHRIEFVRERTGV
                     RYYNDSKGTNPDAAIQALRAMPGPVLLIAGGYDKNSTYDEWAKEFAGRVKYLVLIGKT
                     RDKIAACAKKYGFTEIMYAEDLKEAVQVCAVYADAGDYVLLSPACASWDMFKNYEERG
                     RVFKECVMAL"
     gene            2169..3464
                     /locus_tag="ACZPCL_11295"
     CDS             2169..3464
                     /locus_tag="ACZPCL_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1163085.1"
                     /translation="MAGKKRRKNRPAPAQQAVPVRRGNEQVRYVQPGRPASYPGAGQA
                     YEQPVAQEGKKRKPRHFYDYSLLFIIIFLTVFGLVMIYSASSYTAQLSSKYNGNSAYF
                     MQRQAMIAAIGFFIMLVISKLDYHLLAKVAVPSYALSYALMIAVSLVGRAVNGKRRWL
                     GVGPFSFQPTEFAKIALIIMLASYITVKGDKINQWKYMRNVTFMTLPIAGLVAANNLS
                     SGIILVGIGFVMLFVACKIKWPFYSLAALAMGILAGAGPIGKALMQIKLLQPYQYRRI
                     EAWLNPELDPEGKSFQVLQGLYAIGSGGLFGQGLGQSIQKLGFLPESQNDMIFAIICE
                     ELGLFGAISIILVFLFMIFRFMVIANNAPDLFGALLVVGVMGHIAIQVVLNIAVVTNT
                     IPNTGITLPFISYGGTSVLFIMMEMGIVLSVSNRIRLEK"
     gene            3551..4816
                     /gene="murA"
                     /locus_tag="ACZPCL_11300"
     CDS             3551..4816
                     /gene="murA"
                     /locus_tag="ACZPCL_11300"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR1163086.1"
                     /translation="MAAIQVYGMNPLEGEISVQGSKNGALPIMAGALLHKGTTVLYNV
                     PDIEDTRCMTDILREIGCRCVKTGSRLVIDAVFVDRTEVPGKYVKAMRSSIILLGALL
                     GRMKEGRCSFPGGCLIGARPVDLHLMVLEKLGAIIEEEDGQYKAEAFNGLKGCEIEFS
                     YPSVGATEQAVLAAVLADGVTVIHNAAKEPEISQLCHFLNGMGAVICGMETEHLMVQG
                     VKRLHDSVWKIEGDRIVAGTYSTAVMAAGGNISLKEVNTKQLKLPLELLRKAGANVRE
                     EEFSLHISMKDRPKALSVCTGPYPEFPTDLQSPFMAFLACAKGESRIREQVFEDRFGT
                     AAALRKMGADITCKGKDAWIRGVYPLKGARVKALDLRGGAALAVAALGAEGKTVIEDC
                     GHIVRGYEDICRDINALGGKISWMESDTG"
     gene            4813..>5477
                     /locus_tag="ACZPCL_11305"
     CDS             4813..>5477
                     /locus_tag="ACZPCL_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR1163087.1"
                     /translation="MIRRAERKSYKWVGIAAAVLLLAAVLVFGLRIKEVSVSGSNRYT
                     PEQIENILFSGRWGNNTILAFIRDKTQPHRQIPFVEKYKIVFHGPFKAEVIIYDKSIV
                     GYVSYMSSYMYFDRDGIIVESSPDCLPGVPWITGLEFGRIVLYQHLPVKDPKIFEEIL
                     NLTQQLSVYHIQVDRIRFGSSGQPSLTIGQMEVTLGDGGDVDGKLSLLNDILRDHPEL
                     KDK"
BASE COUNT         1499 a         1070 c         1499 g         1409 t
ORIGIN      
        1 aaaatggtaa attcttatcc cttggtattt tatttgtacc agctctgttt tttatcactc
       61 ttggtacaga taatggtgta aattttacag acggactgga tggcctctgt accagcgtta
      121 ccattctggt agctactttc ctgacgatcg tagccatcgg ggaaaatatg gggatcagtc
      181 ctattacagg agcagtggta ggaagcttac tgggctttct gctttttaat gtttatccgg
      241 caaaggtatt tatgggagat acaggttccc tggctcttgg cggttttgtg gcttccgcag
      301 cttatatgat gcgtctgcct ctgtttatcc cgatcattgg actgatctac ctggtagaag
      361 tgctttccgt tatcgtccag gttacttatt ttaagaaaac agggggaaag cgtatcttta
      421 aaatggctcc gatccatcat cattttgaac tgtgcggctg gtctgaaaca cgggtagtag
      481 ctgtattttc tatcgtaaca gcgcttctgt gcatgatcgc ttacctgggg ctgtaagata
      541 aaagaggcgt agcaaaaact ccgagagaac atcaaaagaa caggaggtta aaagcaacat
      601 gaaccagagc aaggtattgg ttgcaggaac cggcatcagc ggcattgcgg ctgcaaagct
      661 ggttcttgaa agaggcggcg aagtggtgct ttatgacggc aacacagcgt taaatacaga
      721 tgagatccgg gcaaagttcg gaaaagatgc gaaagtggga atcgtccttg gtgagatcaa
      781 gcgctcggat cttttagggg tggaactttg tatcatcagt ccgggaattc ctttaaacag
      841 tgggtttgtg gcagtggtag atgaagcagg gatccctgtt ttgggagaga tcgagttagc
      901 atatcagtgt ggtctgggaa aactggctgc tattacagga accaatggaa agaccactac
      961 aacagctctt acaggcgcga tcttaagatc ccagtatgag gaaacctttg tagtaggaaa
     1021 tatcggcgag ccttatacat ccaaagtttc agagatgaca gaccagtctg tgactgtagc
     1081 ggaagtgagc agcttccagt tggagactat tatggatttc cacccggatg taagcgcgat
     1141 cttaaatatc accccggatc atctggaccg tcacggatct atggaaaatt atatccgcat
     1201 caaagaatgt atcaccatga accagactgc aaaagacgca gtggtcttaa attatgatga
     1261 tcctgtttta agggaatttg gagaaagccg gatcgaagag ccggaacctg cagaggatgc
     1321 agcggataca gaaacagaag ctgtggcccg ggaagacgga ctagatggtg cagaagatct
     1381 tagggagcca gatgagactg caggtgaaga gccagagcag tcagagacag aagttacaga
     1441 aactgtttct gagtcagaag ctgaagaaac ggatccggag gaagttcttt ccggtcttaa
     1501 ggcccgggta ttctggtttt ccagccgtga gcgtctgaaa gagggatttt tcttagatgg
     1561 agacaatatt gtctatgcag acggaacaaa agagcagatc cttgtaaatg taaaggaact
     1621 gcagctttta ggacgccaca attatgaaaa tgtaatggca gctgcctaca tcggcctgtt
     1681 aatgggagta ccttttgaaa agatccggga aacagtccgg aaattcaaac cggttgagca
     1741 caggatcgaa tttgtaaggg agcgtacagg tgtgcgctat tacaatgact ccaagggaac
     1801 caatccggat gcggcgatcc aggcacttag agctatgccg gggcctgttc ttctgatcgc
     1861 aggtggctat gacaaaaaca gcacctatga tgagtgggca aaagagtttg ccggcagggt
     1921 gaagtattta gtcctgatcg gaaagaccag ggataagatc gcagcctgcg cgaaaaaata
     1981 tggtttcaca gagatcatgt atgcagagga cttaaaagaa gcggtgcagg tatgcgctgt
     2041 gtatgcagat gcaggagatt atgtgctgtt aagtccggcc tgcgcaagct gggatatgtt
     2101 taaaaattat gaagaacgcg ggcgcgtctt taaagaatgc gtcatggcgc tttaggaggt
     2161 aaaagacaat ggcgggaaag aaacggcgaa agaaccgccc cgcccctgcg cagcaggcag
     2221 ttcctgtaag gaggggcaat gagcaggtgc gttatgtaca gccgggacgg cctgcttctt
     2281 atccgggagc cgggcaggct tatgagcagc cggttgccca ggagggaaag aaaagaaaac
     2341 cccgtcattt ttatgattac agtcttcttt tcatcattat tttccttact gtatttgggc
     2401 tggtcatgat ctacagtgcc agttcttata ctgcgcagtt aagctccaaa tacaatggaa
     2461 acagtgccta ttttatgcag agacaggcga tgatcgcagc aatcggattt ttcattatgc
     2521 tggtgatctc aaaactggat tatcatttgc tggcaaaggt agcagttcct tcctatgcac
     2581 tgtcatatgc tttgatgata gcagtttcac tggtgggaag agctgtaaac ggaaagagaa
     2641 gatggctggg tgtgggacct ttcagtttcc agcctacaga gtttgcaaag atcgccctga
     2701 tcattatgct ggcgtcctat atcaccgtga aaggtgataa gatcaaccag tggaagtata
     2761 tgaggaacgt gacttttatg acgctcccga ttgccggact ggttgctgcc aacaacttga
     2821 gttccggtat catcctggta ggtattggct ttgtcatgct gtttgtggca tgtaagatca
     2881 agtggccgtt ttacagtctg gcagcattag caatgggcat tttagcaggt gccggaccca
     2941 tcggtaaagc gctgatgcag atcaaattgc tgcagcctta ccagtaccgc cgtattgagg
     3001 catggctaaa tccggagctt gacccggaag gaaaaagttt ccaggtgctt cagggattat
     3061 atgccattgg ttccggcggc cttttcggac agggcttagg acagagtatc cagaagttag
     3121 gtttccttcc tgagtcacag aatgatatga tctttgctat catctgtgag gaactggggc
     3181 tgtttggcgc catatccatt atcctggtgt tcttatttat gatcttccgt tttatggtca
     3241 ttgcaaataa tgcaccggat ctgtttggag ctcttttagt agtgggtgtt atggggcaca
     3301 ttgccattca ggtagtttta aatattgccg tagtaaccaa taccattccc aatacaggta
     3361 tcacccttcc ttttatcagt tatggaggta catcggtgtt gtttatcatg atggaaatgg
     3421 gaattgtttt aagcgtatcc aaccggatcc ggctggaaaa ataagggtca gaaatcagct
     3481 ggccatgcac ataagcagcc atcagggcat atgatcaaag tagatacaca ttttctgcgg
     3541 aggtgccttt ttggctgcaa ttcaggttta tgggatgaat ccactggaag gggagatctc
     3601 tgtacagggc tcaaaaaacg gcgcactccc cattatggcc ggggctctgc tccacaaagg
     3661 gactacagtg ctttataatg tgccggatat tgaagatacc cggtgtatga cagatatttt
     3721 aagggagata gggtgccgct gcgtgaaaac gggttcccgt ctggtgatcg atgctgtttt
     3781 tgtggaccgt acagaggttc cgggaaagta tgtaaaagca atgcgttctt ccattatcct
     3841 tttgggagct ctccttggaa ggatgaaaga gggaaggtgc agctttcccg gcggctgtct
     3901 gatcggtgca agaccggtag atctgcatct gatggtcctt gaaaaactgg gagctataat
     3961 agaagaagag gacggacagt ataaggcaga agcttttaac ggtcttaagg gctgtgaaat
     4021 agaattttcc tatcccagtg taggcgctac ggaacaggcc gtgttggcag ctgttctggc
     4081 agatggggtg acagtgatcc acaatgcggc aaaagaaccg gagatcagcc agttatgtca
     4141 tttcttaaat ggcatgggtg ctgtgatctg tgggatggaa accgagcatc tgatggtcca
     4201 gggagtaaaa agacttcacg acagtgtatg gaaaatagaa ggggaccgga tcgtagccgg
     4261 gacttacagt acggcagtta tggcagccgg gggaaatatt tctttaaaag aagtaaacac
     4321 aaaacagctg aaacttcctc ttgaactttt gagaaaagca ggagcaaatg tacgggaaga
     4381 agagttttct cttcatattt ccatgaaaga ccgtccaaaa gccctttctg tgtgcacagg
     4441 accttatccg gagttcccta cggatcttca atcccctttt atggcttttc ttgcatgtgc
     4501 caaaggagag agcaggatcc gggagcaggt gtttgaggac cgtttcggaa cagcggcagc
     4561 tttaagaaaa atgggagccg atatcacctg taaaggaaaa gatgcctgga tcaggggagt
     4621 atatccctta aaaggagcaa gagtaaaggc actggatcta agaggcgggg cagcgttagc
     4681 agtggctgcc ctgggggcag aaggaaaaac tgtgatagaa gattgcggcc atattgtcag
     4741 gggatacgaa gacatctgcc gggatataaa tgccctggga ggaaagataa gctggatgga
     4801 gagtgatacg ggatgataag aagagcagag agaaaaagct ataaatgggt agggatcgcc
     4861 gcagctgttc tgctgcttgc ggcggtcctg gtgtttggtc tgcgcataaa agaagtaagc
     4921 gtgtcaggaa gtaaccgcta tacgccggag cagattgaaa atatcctgtt ttccggacgg
     4981 tggggaaaca atacgatcct ggcttttatc agggataaaa cacagcccca cagacagatc
     5041 ccttttgtgg agaaatataa gatcgttttt catggtccct ttaaggcaga ggtgatcatt
     5101 tatgacaaaa gcattgtagg ctatgtgtct tatatgagca gttacatgta ttttgacagg
     5161 gatggtatta tagtagaaag ttcaccggac tgccttccgg gtgtgccctg gatcacaggc
     5221 ctggaatttg gccgtattgt tttgtatcag catcttcctg taaaagatcc caaaatattc
     5281 gaggagatct taaatctgac ccagcagctt tcagtctatc atatccaggt agaccggatc
     5341 cgctttggtt cttcaggaca accttccctt accattggcc agatggaggt aacactggga
     5401 gacggaggag atgttgacgg aaaactgtct cttttaaatg atattctaag agaccacccg
     5461 gaattaaagg ataaaaa
//
LOCUS       DBMDUA010000181         5455 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5849_length_5455_cov_2.079006, whole genome shotgun sequence.
ACCESSION   DBMDUA010000181 DBMDUA010000000
VERSION     DBMDUA010000181.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5849_length_5455_cov_2.079006"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /gene="mreD"
                     /locus_tag="ACZPCL_11310"
     CDS             complement(<1..361)
                     /gene="mreD"
                     /locus_tag="ACZPCL_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR1163088.1"
                     /translation="MNNGKIKQILLNILLILLAFTVQNCVFPLLPFLAATPNLLLILT
                     FSFGFIHGRNAGMFYGFLSGLLLDLFYSGPFGFYTLIYVYIGYFNGIFTKYYYEDYIT
                     LPLILSILNGLWYSLYIY"
     gene            complement(361..1203)
                     /gene="mreC"
                     /locus_tag="ACZPCL_11315"
     CDS             complement(361..1203)
                     /gene="mreC"
                     /locus_tag="ACZPCL_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR1163089.1"
                     /translation="MESNRSKYILAGLTAFCILLIGITSLKDGIMEPLRTGVGYFLIP
                     IQSGVNKVGTGLYNELTDYGKLKAALAENENLKTQVAQLTEDNNRLQAEQFELNRLRQ
                     LYKLDQEYMQYNKIGARVIARDSEKWFQVFRINKGSSDGVAVDMNVVADGGLVGIVTD
                     VGANYATVRSIIDDSSRVGAMSLDSSYNCIVAGDLTLYEQGRLKLTDFSRDAVLRNGD
                     QIITSNISTKYLPGILIGYAVDVSIDPDHLTQSGYLIPAADFDNLQEVLILTDLKNSD
                     EAVE"
     gene            complement(1232..1927)
                     /gene="radC"
                     /locus_tag="ACZPCL_11320"
     CDS             complement(1232..1927)
                     /gene="radC"
                     /locus_tag="ACZPCL_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR1163090.1"
                     /translation="MQKLTMKQLPKEDRPYEKCLESGATSLTDAELLAVIIRTGSREE
                     TSLELARQILSLEGQENRLLGLLHHSLEDYCRIKGVGKVKGAQLMCIGELSRRIWNCK
                     ARETPVSFTHPEQIADYYMEDMRHLEQEEIRVMFLNTKQNLIRDLLVSKGTVNASVVT
                     PREILIEGLRCLAVSMILVHNHPSGDPSPSESDILLTRRVQEAGNVIGISLLDHIIIG
                     DKRFLSFRQQQII"
     gene            complement(2110..2361)
                     /locus_tag="ACZPCL_11325"
     CDS             complement(2110..2361)
                     /locus_tag="ACZPCL_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HIR1163091.1"
                     /translation="MGRKNFWILLIMLLAGIVLGGFMGQLANGISWLSWLNFGQSFGL
                     DSPLVINFGILVITLGLTIKITMASIIGIAIALIIYRMI"
     gene            complement(2408..3703)
                     /locus_tag="ACZPCL_11330"
     CDS             complement(2408..3703)
                     /locus_tag="ACZPCL_11330"
                     /EC_number="4.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000096 - sulfur amino acid metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HIR1163092.1"
                     /translation="MEQNVLDGMYEQLGISKEVLDYAADIEKSLKERFERIDQTAEYN
                     QLKVLKGMQDNKVSDIHFAATTGYGYNDLGRDTLEDVYASVFHAESALVRPQLISGTH
                     ALHIALSGNLRPGDELLSPVGKPYDTLEEVIGIRDSVGSLKEYGVSYRQVDLLPDGTF
                     DYENIAKAINEKTKLVTIQRSKGYDPRPTFSVKQIGELIAFVKKIKPEVICMVDNCYG
                     EFVETIEPTDVGADMIVGSLIKNPGGGLAPIGGYIAGKKECVDRASYRLSAPGLGKEV
                     GASLGLNQQLYQGFFLSPTVVAGALKGAIFAANVYERLGYGVVPNGSESRHDIIQAIT
                     FGSAQGVISFCKGIQAAAPVDSFVTPEPWDMPGYDSQVIMAAGAFVQGSSIELSADGP
                     IKPPYAVYFQGGLTWYHAKLGILKSLQQLLDDGVLKNLR"
     gene            complement(3750..4718)
                     /gene="miaA"
                     /locus_tag="ACZPCL_11335"
     CDS             complement(3750..4718)
                     /gene="miaA"
                     /locus_tag="ACZPCL_11335"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR1163093.1"
                     /translation="MTSVKQPLIILTGPTAVGKTALSIELAKAVGGEIISADSMQVYR
                     HMDIGSAKVTKEEMEGIPHYLIDVLDPQDDFNVVTFQTMAKQAMNEIYSHNKVPIITG
                     GTGFYIQALLYDIDFKENDEKNPVREELEQLAEKLGENAPQVLHQKLQEVDPEAAAQI
                     HANNIKRVIRAIEYFKQTGEKISQHNEEMHQKDSPYNFLYYVLTRDRKVLYERIDRRV
                     DIMMENGLVDEVKKLKDMGCHRGQTSMQGLGYKEILDYLDGGCTLEEAVYILKRDTRH
                     FAKRQLTWFRREKDVRWLDLDQYQGNADLILADILKDCEQAEIVLK"
     gene            complement(4729..>5455)
                     /locus_tag="ACZPCL_11340"
     CDS             complement(4729..>5455)
                     /locus_tag="ACZPCL_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair MutL family protein"
                     /protein_id="HIR1163094.1"
                     /translation="REQSCSRNGETEKDSNTVLDSSKPVSDTRPVTAPKQLDFFGEKL
                     LDPASRLKHKLIGQLFDTYWLIEYNDHLYIIDQHAAHEKVLYEKTVKSLKDRDYSSQM
                     VEPPIILTLNPNEEFLLNKYMEYFTRIGFEIEPFGGREFAVRAVPANLFSVAKKELLL
                     EMIDGLSDEIAARNPDSIYEKIASMSCKAAVKGGNHLSAMEANELIDQLLKLDNPYAC
                     PHGRPTIISMSRYELEKKFKRIV"
BASE COUNT         1454 a         1271 c         1152 g         1578 t
ORIGIN      
        1 cataaatgta taagctgtac cataaaccat ttaaaatact taagatcaga ggaagggtga
       61 tataatcctc ataataatat ttggtaaaaa taccgttgaa ataaccaatg tatacgtaga
      121 tcagcgtata aaagccaaat ggcccgctgt agaaaagatc cagcagcaga ccagataaaa
      181 agccgtaaaa catgcctgcg ttccgtccgt ggataaaccc gaaggaaaag gtaaggatca
      241 gaagcaggtt gggtgttgcc gccagaaaag gcagcagggg gaatacgcag ttctgcacag
      301 taaaagccag gaggatcaaa agtatattta agaggatctg tttgattttt ccgttattca
      361 ctattctacc gcctcatcac tgtttttaag atctgttaag atcagaactt cctgaagatt
      421 gtcaaaatcc gcagccggga tcagataacc ggactgggtc agatgatccg gatcaatgga
      481 tacatccact gcatagccga tcagaatacc cggaagatat ttggtactga tattggaagt
      541 aatgatctgg tctccgtttc taagcactgc gtccctggaa aaatctgtaa gttttaatct
      601 tccctgttca taaagggtca ggtcaccagc aacaatacag ttataggaag agtccagtga
      661 catagcgcct acacggctgg aatcatcgat aatggaacgg accgtagcat aattggcacc
      721 tacgtctgtt acgataccta ccagaccacc gtctgctact acgttcatat ccacagcaac
      781 cccgtcagag gatcctttat tgatacggaa cacctggaac catttttcag agtctctggc
      841 gatcactctt gcgccgatct tgttgtactg catgtactcc tggtctaact tgtacagctg
      901 gcgcaggcgg tttaactcaa actgctctgc ctgaagcctg ttattgtctt ctgtaagctg
      961 ggcaacctgg gttttcaggt tttcattttc tgccagggca gcttttaatt ttccataatc
     1021 ggtaagttca ttgtaaagac cggtacctac tttattgaca ccggactgta tagggatcag
     1081 aaagtatccc acgccagtac ggagcggttc cataatacca tcttttaaag aagtgatacc
     1141 aataagcagg atacagaagg ctgtaagacc tgccagtata tatttgctgc gatttgattc
     1201 catggatatc tgttttcctt tacatgtata actaaatgat ctgctgctga cgaaagctta
     1261 aaaaacgctt atccccgata ataatatggt ccaaaagaga aatacctatt acattgcctg
     1321 cctcctgtac cctgcgggta agaaggatat ctgactcact gggagaagga tccccgctgg
     1381 gatgattatg gaccaggatc atgctcactg ccaggcagcg gagcccctcg atcaaaatct
     1441 ccctgggcgt aaccacagaa gcattgactg tgccttttga gaccagaagg tcccggatca
     1501 gattctgctt tgtatttaag aacatgaccc ggatctcctc ctgctctaaa tggcgcatat
     1561 cttccatata atagtctgca atctgctccg gatgagtaaa ggaaaccggt gtttccctgg
     1621 ctttgcagtt ccagatccgc ctggaaagtt caccgatgca cataagctgg gcacctttta
     1681 cctttcccac gcctttgatg cggcagtaat cttccaggga atgatgcaaa agccccagca
     1741 gccggttttc ctgcccttcc aaagacagga tctgtctggc aagctccagg gaagtttcct
     1801 ctctgcttcc ggtgcgtatg ataacagcta aaagttctgc gtctgtcagg gaagtggcac
     1861 ctgactcaag gcatttctca taaggcctgt cttcttttgg aagttgtttc attgttaatt
     1921 tctgcatatg ctctcctcct gtaaaaggca cctctccaag tacgcaaagc agaaggattt
     1981 cacttatcgt aaactcacga atcccgtact tacgtgctct attgcctgct tacgcaggtg
     2041 attcgttcgt taatggtttg cgaaaagcaa atgtctgctt acgcagccgc ttgtttttcg
     2101 ctgcacacat taaatcatac gataaatgat cagggcaatg gcaatgccga taatacttgc
     2161 catagtgatc ttgatggtaa gtcccagagt aatgaccagt atgccaaagt taatgactaa
     2221 aggtgaatca agaccaaatg actggccaaa attcaaccag ctcagccatg aaatgccgtt
     2281 ggcaagctgt cccataaagc ctcctaatac aatgcctgca agtaacatga tcagaaggat
     2341 ccagaaattc ttacgtccca taaacactct ccttaagtta tcagtttacc ataagttgtt
     2401 tttcatgtca tcttaagttt ttcaaaactc catcgtctaa aagctgctgt aaagacttaa
     2461 gaattcccag ttttgcatgg taccaggtaa gtccaccctg gaagtaaacg gcatatggag
     2521 gcttaatagg accatcagca cttaattcaa tggaagatcc ctggacaaat gcacctgcag
     2581 ccataatgac ttgactgtca taacctggca tatcccatgg ctctggtgtt acaaagctgt
     2641 ctacaggagc agctgcctga attcctttgc agaaagagat cacaccctgg gcagaaccga
     2701 aggtgatcgc ctggataata tcgtgacggg attcactgcc atttggaaca acgccgtatc
     2761 ccagtctttc gtatacatta gctgcaaaga tcgcaccttt taaggcacct gcaactacag
     2821 tcggggataa gaaaaatccc tgatacagct gctggtttaa accaaggctt gcgcctactt
     2881 cttttccaag gcctggagcg cttaaacggt aagatgcacg gtctacgcac tctttctttc
     2941 ctgcaatata accgccgata ggagcaaggc cgcctcccgg gtttttgatc agggaaccta
     3001 cgatcatatc agctccaaca tcggtaggtt caatagtctc tacaaattca ccatagcagt
     3061 tatctaccat acagataact tccggtttga tcttctttac aaaagcgatc agctcgccga
     3121 tctgttttac agaaaaagta ggacgaggat cataaccttt ggaacgctgg atggtcacta
     3181 attttgtctt ttcattaatg gcttttgcga tattttcata atcaaaggtg ccatccggca
     3241 gaagatctac ctggcggtag cttacgccgt attcttttaa agaacctacg gagtcgcgga
     3301 taccgatgac ttcttccaaa gtatcatatg gcttgccaac aggggaaagc agttcatctc
     3361 ccggacgtaa gtttccggat aatgcaatgt gaagggcatg ggttccggag atcagctgag
     3421 gacgtaccag ggcgctttca gcatggaaaa cactggcata aacatcctct aatgtatcac
     3481 ggccaaggtc attgtaacca taaccggtgg ttgcagcgaa atggatatcg ctgactttat
     3541 tatcctgcat tccttttaag acctttaact ggttgtattc tgcagtctgg tcgatccgct
     3601 caaaacgctc cttcagactt ttttctatat ctgcggcata atcaagaact tctttgctga
     3661 tacccagctg ttcatacata ccgtctaaaa cattctgttc cattatgtga taactcctat
     3721 ctttattaaa ttgctctttt ttcggtttgc tattttagca cgatttctgc ctgttcgcaa
     3781 tcctttaata tatctgcaag gataaggtct gcatttccct gatactggtc taagtccagc
     3841 cagcgcacat ccttttccct gcgaaaccat gtaagctgcc gcttggcaaa atgtctggta
     3901 tcccgtttca ggatatatac ggcttcttct aacgtgcagc caccatccag ataatcaagg
     3961 atctccttat atccaagccc ctgcatggaa gtctgtcccc tgtgacagcc catatctttt
     4021 aatttcttta cttcatctac cagaccgttt tccatcataa tatccacacg ccggtcaatg
     4081 cgctcgtata aaactttgcg gtccctggtg agaacatagt ataagaaatt atagggagag
     4141 tctttctggt gcatttcttc gttgtgctgt gagatttttt ctcctgtctg tttgaaatat
     4201 tcaattgcac ggatcacacg ctttatattg tttgcatgga tctgtgcagc agcttccgga
     4261 tccacttcct gcagtttttg atgaaggacc tgaggcgcat tctcacccag tttttctgcc
     4321 agctgttcta actcttcacg aacaggattt ttctcatcat tttctttaaa atcaatatca
     4381 taaagcagag cctggatata aaatcctgtg cctccggtaa tgatcgggac tttgttgtga
     4441 gaataaatct cattcattgc ctgtttggcc attgtctgga atgttactac attaaaatcg
     4501 tcctgaggat ctaaaacgtc gatgagataa tgaggaattc cctccatttc ttctttcgtt
     4561 acctttgcag aaccaatatc catatggcga tatacctgca tggagtctgc gctgatgatc
     4621 tctcctccta ctgcttttgc aagttcaatc gaaagagcgg tttttcctac agccgtaggt
     4681 cctgtaagga tgataagagg ctgttttact gatgtcatgg tctctccttt atacaatgcg
     4741 cttaaatttc ttttccagtt cgtaacggct catggagatg atggtggggc gtccatgagg
     4801 gcaggcatag ggattgtcca gctttaacag ctgatcgatc agttcatttg cttccatggc
     4861 ggataagtgg ttgccgcctt ttacagctgc tttgcaggac atggaagcaa tcttttcata
     4921 aatactgtcc gggttgcggg ctgcgatctc atcggaaaga ccatcgatca tttccagaag
     4981 aagttctttc tttgcaacgg aaaacaggtt ggcaggtact gcacggactg caaattcacg
     5041 accgccaaat ggctcgatct caaaaccgat ccttgtaaaa tattccatat acttatttaa
     5101 aagaaattcc tcatttggat ttaaagtcag gatgatcggc ggctccacca tctgggagct
     5161 gtagtccctg tcttttaagc tttttactgt cttttcatag agaacttttt catgggccgc
     5221 atgctggtcg atgatatata aatgatcgtt gtattcgatc agccagtaag tgtcaaataa
     5281 ttgaccgatc agcttgtgtt tcagacggga cgccggatcc aagagttttt ctccgaaaaa
     5341 gtccagctgc ttgggagctg taactggtct ggtatcagaa accggttttg aactgtcaag
     5401 gacagtattg ctatcttttt cggtttcccc gtttcgggag cagctttgtt ctctt
//
LOCUS       DBMDUA010000182         5363 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5940_length_5363_cov_2.625428, whole genome shotgun sequence.
ACCESSION   DBMDUA010000182 DBMDUA010000000
VERSION     DBMDUA010000182.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5940_length_5363_cov_2.625428"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="ACZPCL_11345"
     CDS             complement(<1..954)
                     /locus_tag="ACZPCL_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR1163095.1"
                     /translation="MKTFWNTYKSSILLLAAMVAGGVIGFFWGPGASVLQPIADTFLN
                     LLYCCVVPLIFCSLTAAIAKMQDLAKLRKILMIFLIGVIVSGVIACLFMVAPCLVIDP
                     AEGSTVSLTQDVADASGSMNVLGMFTVGDFPLLFSKSNLMALIVFTVIFSIAIIQTGE
                     KGKPIVEAMDRLTAAIVNVINIVMKLAPLGLGCYFAILIGSNGSDVIGPLSKAIVMYL
                     FVIVAYYVVSQTVFAYIGGGMEGVRIWWATCVPSTLTALGTCSSAATLPVNLQQAKKI
                     GIPDEIADLVIPLGANLHKDGACVIQIIKVAFLCSVFHMEYA"
     gene            complement(973..1842)
                     /locus_tag="ACZPCL_11350"
     CDS             complement(973..1842)
                     /locus_tag="ACZPCL_11350"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF012382.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR1163096.1"
                     /translation="MHKEGDALLIQQPVYYPFREVIEDNGRIFVNNQLHYEDGKYTID
                     FEDFEKKIVDNNVKVFILCNPHNPVGRVWTKEELEKMGDICLKHNVVIMDDEIHCDFT
                     YPGFKFTSFMTLDEKYHNSLVLYTSPSKTFNVAGLQPANIIIKNPELRAAYRKANAAA
                     GYSQGSIMGQVAVKSVYTKGDEWVNELVEYITGNMNYMRDFVKEYFPKAHFVDPEGTY
                     LTWVDFSGYGFTDEELEHIMVEEAHLWLDSGKVFGPATAQFERFNLACPRATVVKAME
                     QLKAALDNHTKWA"
     gene            1837..2289
                     /locus_tag="ACZPCL_11355"
     CDS             1837..2289
                     /locus_tag="ACZPCL_11355"
                     /inference="COORDINATES: protein motif:HMM:NF024854.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1163097.1"
                     /translation="MHYNDYIMSAHQYGDNYTLTMMEVHTLSYIEDHPGTSSGELIQY
                     WDKTKGTISQILTKLEKLELIEKRKKDGNAKNLYLYVTAEGSRVSKAHKMYDILDIAK
                     TMSQLQKQCSAEEIEAFYHVISVYNEVIKKDFEINKSPRKRTDVTPLP"
     gene            complement(2443..5019)
                     /locus_tag="ACZPCL_11360"
     CDS             complement(2443..5019)
                     /locus_tag="ACZPCL_11360"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1163098.1"
                     /translation="MKQYTVTGMSCAACSTRVEKAVSKVPGVTSCSVSLLTNSMGVEG
                     SATDQEIIKAVADAGYGASAKDAEGEKNGGANSAAASEEALADHETPILKKRLLYSVG
                     FLLVLMYFSMGHMMWNWPLPAFMDGNHVMMGLVQLYLTVIIMVINQKFFINGFKSLFH
                     GAPNMDTLVALGSFASFGYSSYALFAMTYAEHQGDAEAVMGYMHEFYFESAAMILTLI
                     TVGKMLEARSKGKTTDALKSLMKLSPKTAVVEKEGKETEVPVEQVRIGDVFVVRPGEN
                     IPVDGVVLEGNSAVNEAALTGESIPVDKQAGDRVSAATINQSGFIRCEATRVGEDTTL
                     SQIIRMVSDAAATKAPIAKVADKVSGIFVPAVISIAVLTVIVWLIAGESVGFALARGI
                     SVLVISCPCALGLATPVAIMVGNGMGAKNGILFKTAVSLEETGKVEIVALDKTGTITS
                     GEPKVTDVLAADGTSEEELLKLAYSLESRSEHPLAKAIVAYGAEKQAEQIPVSEFKAL
                     PGNGLEGKVGDADVKGGSLKFAQSQTEISEKIKSQAEKLAEEGKTPLMFLKNGILAGM
                     IAVADVIREDSPQAVKELQNMGIEVVMLTGDNERTAKAIGRQAGVDRVIAGVLPDGKE
                     EVIRRLKEQGKVAMVGDGINDAPALTRADMGIAIGAGADVAIDAADVVLVKSRLSDVP
                     AAIRLSRGTLRNIHENLFWAFFYNVIGIPLAAGIWIPIFGWKLNPMFGAAAMSLSSFC
                     VVTNALRLNLLNIKDSRKDKKIRRKAAAPAGAVVAAAEINTETKKENKTMTKTMKIEG
                     MMCGHCEAAVKKALEALDGVASAEVSHEKGTAVVTLDKDVDNAVLTKAVEDKDYKVVS
                     VQ"
     gene            complement(5127..>5363)
                     /locus_tag="ACZPCL_11365"
     CDS             complement(5127..>5363)
                     /locus_tag="ACZPCL_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HIR1163099.1"
                     /translation="ISCLNRIEGQIRGIKGMVEKDAYCPDILIQVAAANAALNSFNKV
                     LLANHIRTCVAEDIRAGKDEVIDELVTTLQKLMK"
BASE COUNT         1404 a         1349 c         1043 g         1567 t
ORIGIN      
        1 tgcatattcc atatggaata ctgagcataa aaacgctacc ttaatgatct ggatcacaca
       61 tgcgccatct ttgtgaagat ttgcacctaa aggaataaca agatctgcaa tctcatcggg
      121 aataccaatt ttctttgcct gctgtaaatt aactggcaga gttgctgctg aagagcaggt
      181 accaagagca gttaaggtag atggcacgca ggttgcccac catatgcgta caccttccat
      241 gccgccaccg atataagcaa atactgtctg tgaaacaaca taataggcaa cgatcacaaa
      301 caggtacatg acaattgctt ttgacaacgg tccgatcaca tcactgccgt tagaaccgat
      361 caggatcgca aaataacagc caagtcctaa aggggccagt ttcataacga tattgatcac
      421 gtttacgata gctgctgtaa ggcgatccat agcttctacg atcggcttgc ccttttcacc
      481 ggtctggatg atcgcaatag agaaaataac agtaaatacg atcagtgcca tcaggttaga
      541 tttagagaac agaagcggga aatcaccgac tgtaaacatt cccagtacat tcatgcttcc
      601 actggcatct gctacatcct gtgttaaaga aacagtagaa ccttccgccg gatcaatgac
      661 cagacaagga gctaccataa acaggcatgc aataacaccg gagacaataa caccgatcag
      721 gaagatcatt aagatctttc tgagttttgc aagatcctgc atttttgcga tcgccgctgt
      781 taaagaacag aagatcagcg gtacaacaca gcaatacagc agatttaaaa atgtatcggc
      841 aatgggctgg agtacacttg ctccaggtcc ccagaaaaat ccaatcacac cgcctgctac
      901 catagctgcc agcagtaaaa tagacgattt atatgtattc cagaaagttt tcatctctta
      961 tcttccccct gactatgccc actttgtgtg gttgtccagt gcagctttta actgctccat
     1021 tgcctttact acggtagctc tcgggcatgc aaggttaaat ctttcaaact gtgcagttgc
     1081 cggtccgaat accttaccgc tgtctaacca gagatgtgct tcttctacca taatgtgctc
     1141 taattcttca tctgtaaaac catatcctga aaaatcaacc caggtaagat aggttccttc
     1201 aggatctacg aaatgagcct tcggaaagta ttcctttacg aaatcacgca tgtaattcat
     1261 atttccggta atatactcta ccagttcatt tacccattca tcaccctttg tataaacaga
     1321 ttttactgca acctgtccca taatgcttcc ctggctgtaa cctgctgctg catttgcctt
     1381 acggtatgct gcgcggagtt ccggattttt aataataatg ttggctggct gaagtcctgc
     1441 tacgttaaaa gtcttacttg gagaagtata cagtaccaga ctgttatggt acttttcatc
     1501 cagagtcatg aagcttgtaa atttgaagcc aggatatgta aagtcacaat ggatctcatc
     1561 atccatgatc actacattgt gctttaagca gatatctccc attttctcca gttcttcttt
     1621 tgtccatact ctgccaacag ggttgtgagg attgcacagg ataaatactt ttacattgtt
     1681 atctacgatc ttcttttcaa aatcttcaaa atcaatggtg tattttccat cttcataatg
     1741 cagctggtta tttacaaaaa ttcttccgtt atcttcgatc acttcgcgga atggatagta
     1801 aaccggctgc tggatcagaa gcgcatcgcc ttctttatgc attataatga ctacatcatg
     1861 tccgcacacc agtatggtga taactatacg cttaccatga tggaagtcca cacactcagt
     1921 tatattgaag accatcccgg aacctcttcc ggtgaactga tccagtactg ggataaaaca
     1981 aagggaacta tttcccagat ccttactaaa ttagaaaaac tggaactgat cgaaaagcgc
     2041 aaaaaagacg gaaacgcgaa aaacctttat ctatatgtga cagcagaagg ttcccgtgtc
     2101 agtaaggcac ataaaatgta tgatatcctg gatatcgcca agactatgtc ccagcttcag
     2161 aaacagtgca gcgccgaaga gatcgaggca ttttaccatg tgatctctgt ttacaacgaa
     2221 gtcattaaaa aagattttga gatcaacaaa tccccacgca aacggacaga cgtaacacct
     2281 cttccctgat ttttttatat actaaattta taaaaagaat gtgccgtggc acaaaaaacg
     2341 cagaaaccca tcttttcgga ccggttcctg cgtttctttc attcatttta tactattttg
     2401 tattttatac tattttattc atttttgttc atctatgatc gcttactgaa cagaaactac
     2461 cttataatcc ttatcttcta ctgcctttgt aaggactgca ttatctacgt ccttatctaa
     2521 ggttacaaca gcagttccct tttcgtggct tacttctgcg ctcgctactc cgtctaatgc
     2581 ttccagagcc ttcttaacag ctgcctcgca gtgtccgcac atcattccct caattttcat
     2641 agtctttgtc attgttttgt tctccttttt tgtttctgta tttatttctg ccgcagcaac
     2701 tactgctcct gcaggagcgg ctgctttacg gcggatcttc ttatctttgc ggctgtcttt
     2761 gatatttaat aaattcagcc ttaatgcatt ggtcacaacg cagaagctgg ataagctcat
     2821 ggctgctgca ccaaacatcg ggtttaactt ccatccgaag atggggatcc agatacctgc
     2881 tgcaaggggg ataccgatca cattatagaa gaaggcccag aacaggtttt catggatgtt
     2941 tctaagggtt ccgcggctta aacggatggc tgcgggcaca tcgcttaagc ggctctttac
     3001 cagaacgaca tctgctgcgt caatggcaac gtctgcaccg gctccaatgg caatacccat
     3061 atccgctctt gtgagagcag gggcatcatt gataccgtca ccaaccatgg caacttttcc
     3121 ctgttctttt aacctgcgga tcacttcttc cttaccatcc ggaagaacgc ctgcgatgac
     3181 acggtctacg ccggcctggc ggccaatggc cttggctgtg cgttcattat caccggtcag
     3241 cataactact tcaataccca tattctgcag ctcttttact gcctgtgggc tgtcttctct
     3301 gatcacatcg gcaactgcga tcatacctgc aagtatacca ttttttaaga acataagagg
     3361 tgtttttccc tcttctgcca gtttttctgc ctggcttttt atcttttcgg atatctccgt
     3421 ctggctctgt gcaaatttca ggctgccgcc ttttacatcc gcatctccca cttttccttc
     3481 caggccattt cccggaagtg ccttaaattc actaaccggg atctgttctg cctgtttttc
     3541 tgccccatag gctacgatcg cctttgctaa cggatgctcg ctcctgcttt ccagtgaata
     3601 tgctaatttt aacagctctt cttctgaagt tccatctgcc gccagtacat ctgttacttt
     3661 tggctcacca cttgtaatgg ttcctgtttt atctaatgca acgatctcaa cttttcctgt
     3721 ttcttccagg gaaacggcag tcttaaagag gataccgttc tttgcaccca taccatttcc
     3781 taccatgatt gcaaccggtg ttgcaagtcc caaagcacaa gggcagctga taaccagaac
     3841 agaaatacct cttgcaaggg caaaaccaac gctttcacct gcgatcagcc atacaataac
     3901 cgtcagcact gcaatggaaa taacagcagg aacgaaaata ccggaaactt tatctgccac
     3961 ttttgcgatg ggtgccttgg tagctgccgc gtcactgacc atacggatga tctgggagag
     4021 tgtagtatct tctcctacgc gggtagcttc acagcggata aaaccggact ggttaatggt
     4081 ggctgcggaa acacggtctc ctgcctgctt gtctacagga atactttcac ctgtaagagc
     4141 agcttcgttt acagcgctgt ttccttctaa tacgacacca tctaccggta tattttcacc
     4201 agggcggaca acaaatacat caccgatgcg tacctgttca acaggtactt ctgtttcttt
     4261 tccctctttt tccactacgg cagtctttgg tgacagcttc atcaggctct ttaaggcatc
     4321 tgtagtcttt cctttggaac gggcctctaa catttttccg actgtgatca gtgtcaggat
     4381 catggcagcg gactcaaagt agaattcatg catgtagccc atcactgcct ctgcatctcc
     4441 ctgatgttca gcataagtca tggcaaacag tgcataggag ctgtagccga aggaggcaaa
     4501 ggaacctaag gctactaagg tgtccatgtt tggagcacca tggaacaggc tcttaaaacc
     4561 gttaataaag aacttctggt ttatgaccat aatgatcact gtcagataca gctgtaccag
     4621 acccatcatc acatggttgc cgtccataaa tgccggcagc ggccagttcc acatcatatg
     4681 tcccatggag aaatacataa ggacaagtaa aaaacctacg gaatataata aacgtttctt
     4741 taaaatagga gtttcatggt ctgcaagggc ctcttcagaa gctgcagcgc tgtttgctcc
     4801 accgtttttt tctccttcgg catcttttgc agacgcgcca tagcctgcat ctgctactgc
     4861 ttttatgatc tcctggtctg tggcacttcc ttccacgccc atggagttgg ttaaaaggct
     4921 gacagagcag gaagttactc ccggcacctt ggaaaccgcc ttttctacac gggtgctgca
     4981 ggctgcacag ctcattcccg ttactgtata ctgcttcatt gtattactgg ctcctttctt
     5041 atacctgttc atacctgttt aggttccaca cacggtcgtg ctccactgcc tgctgacgca
     5101 ggcggaacct tcattttcct ctccacttat ttcataagtt tctgcaaggt ggtcaccagt
     5161 tcatcgatca cctcatcttt tcccgctctt atatcttctg ctacacaggt cctgatatga
     5221 tttgcaagaa ggaccttatt aaaactgttt aacgctgcat tggctgcagc cacctgtatt
     5281 aatatgtccg gacaataagc gtccttttcc accatccctt taattccccg tatctggcct
     5341 tcaatgcggt ttaagcatga aat
//
LOCUS       DBMDUA010000183         5352 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5948_length_5352_cov_2.458960, whole genome shotgun sequence.
ACCESSION   DBMDUA010000183 DBMDUA010000000
VERSION     DBMDUA010000183.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5948_length_5352_cov_2.458960"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(222..1073)
                     /locus_tag="ACZPCL_11370"
     CDS             complement(222..1073)
                     /locus_tag="ACZPCL_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1163100.1"
                     /translation="MEKKNVIGNGTKTENGNENGSGIGMGTANDLEVSGIKFHYGKRE
                     ILKGTGFCARRGEVCLLLGANGAGKSTLLKCINGLLKASDGTVKWLGQDTKPLSLREK
                     AKIFGYVPQNTQAGLSLTVMETVISGRLPFSGGKTGKEDVEKSAQVLEQFGLSDYAFR
                     QMGQLSGGERQRILIARALAGDPQVLLLDEPTSSLDLRYQYEVMELLVKVSREKNLAV
                     AAVIHDLNLALDFADRVALLHDGHVLAEGATKEVITPERIEKAYGIRAAIHNLEDRWI
                     VLPEKSL"
     gene            complement(1073..2161)
                     /locus_tag="ACZPCL_11375"
     CDS             complement(1073..2161)
                     /locus_tag="ACZPCL_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR1163101.1"
                     /translation="MRADDNQEKRDLAGDYLRRARRKNILLAVLLLVLLVAAIWAACL
                     GVAQLTPDRMIVTWLPSFSGMFKGVAPLDAKEQNVLLMLRLPRIAAAVIAGAGLGIAG
                     SGMQAITGNPMASPFTTGLSSAAAFGAALMILFGGFPLWMQKGATVAAAFVMSVICAI
                     VVYTVASYKQLRPEALVLTGIALNYLFNALNSTMQFIANEQQLPAIVHWSFGSLTAVT
                     WSDIGIMLFFFAVSFPVFMSQAWAFNLLDDGGDETARALGINVKRTRLLCGGAVTLLT
                     ASVVSFTGIIGFVGLVAPHMARMLIGGDYRHLLPYSAVTGAFLVLAADTLGRNAFSPT
                     TVPVGIVVSYVGVPLFLYLILRERRKSL"
     gene            complement(2167..3318)
                     /locus_tag="ACZPCL_11380"
     CDS             complement(2167..3318)
                     /locus_tag="ACZPCL_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013648.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1163102.1"
                     /translation="MRNSKRWLAAILAGAMIAVTGCSGSASKQETTAAETTVAETTAE
                     ETTAAETTAEAKEGETTTFVDDLGREVELELPVTRACVANRYNNELIRAVGAGDAVVG
                     VDQYTSQDPVYWPQFGEDETFGKDEYDYEKIVALDPQVLVVPKNGKVEESVEKLESFG
                     IKVVVITGWDNSDVPKQIRLCGELFGKQEQAEELVDFYQKPVDYINEQLKQVTDRKKV
                     YWEYGDDYSTCIPGTSNDGWHNMILMAGGINIFGDASLAGKDIDPEQILTEDPDLIVK
                     IYAGKNVIGNSGVFTAPPQEEFAEKADEMLQRPGWKDLKAVKEGNFYLNTAFMSGGLG
                     KMIGAAYMAKWLYPDLMTDLDPDAIFTQWMKYQGFEPNAPHYYHVPAQG"
     regulatory      complement(3529..3710)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3802..5145)
                     /locus_tag="ACZPCL_11385"
     CDS             complement(3802..5145)
                     /locus_tag="ACZPCL_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1163103.1"
                     /translation="MERNLTEGSVFKNIIYFSLPYLLSYFLQTLYGMADLFIIGQFEG
                     VASTTAVSIGSQVMHMLTVMIVGLAMGATINIGQAIGARDKKRAATDTGNTATLFMVL
                     SVVLTVVLFLSVNAIVSIMSTPEEALAGTALYLKICFLGIPFITAYNIISSIFRGMGD
                     SKSPMYFIAIACVVNIALDYVFMGVLHLGPSGAALGTTLSQTVSVIVALTMFMKGKTG
                     ITVKKSDFKPRKETMGRLLKIGFPIAMQDGLIQVAFIIITIIANRRGLTDAAAVGIVE
                     KVISFLFLVPSSMLSTVSALGAQNVGAGKPERAAATLKYAICIAGGFGLIIAIIIQFT
                     AGNVVNLFTNDQAVVYAGAQYLKGYIWDCMFAGIHFSFSGYFCACGRSGYSFIHNITS
                     IALVRVPGVYLTSKIFPETLFPMGLATAGGSFMSVVFCTGLFLLLQRQKRQEKGI"
BASE COUNT         1422 a         1341 c         1044 g         1545 t
ORIGIN      
        1 gatgttatat ccacaagcgt tttatacatt cttctattca tcgccgggat acacataaaa
       61 tatgttccgg cgccttttaa gtttgtgaaa agtggtgccc ccagttcaca aagcagaaat
      121 ggtactgtgt ccatttccta ctttttaata ttacactaac ttagaatttt tttcaataag
      181 atttttctga atattacaaa atattgcaaa attctacact attacaacga tttttccggc
      241 aatacgatcc atctgtcttc cagattatgg atcgcagccc gtatcccata ggctttttcg
      301 atccgctctg gtgtgatcac ctctttggtg gctccttctg ccagcacatg accgtcatgg
      361 agcaatgcca cccggtctgc aaaatcaaga gccaggttta gatcatggat cacggctgct
      421 actgccagat tcttttcccg gcttactttt accagaagtt ccataacttc atactgatag
      481 cgaagatcca ggctgctggt aggttcgtct aaaagcagca cctgaggatc tcctgccagg
      541 gctctggcga tcaatattct ctggcgttct ccaccgctta actgtcccat ctggcggaaa
      601 gcatagtcag acagtccaaa ctgctctaat acctgggcac ttttttccac atcttctttt
      661 cctgtttttc cgccggaaaa tggaagccgc ccggaaataa ctgtttccat aacagtaagg
      721 cttaaaccgg cctgtgtatt ctgtggaaca tagccgaaga tctttgcctt ttccctgagg
      781 gaaagaggtt ttgtgtcctg tcccagccat tttacagttc catcagaggc ttttaaaaga
      841 ccgttgatac attttaataa agtagatttt cctgctccat tggcacctaa cagcaggcat
      901 acttcccctc gtctggcgca gaaaccggtt ccttttaaga tctcacgttt tccataatgg
      961 aacttaattc cggaaacttc caggtcattg gcagttccca ttccaattcc cgatccattc
     1021 tcatttccat tttcggtttt ggttccattt ccaatcacat ttttcttttc catcacaggc
     1081 tcttcctcct ttcacgaagg atgaggtaca ggaacaatgg aacacctaca taggaaacaa
     1141 caattcccac cggtactgta gtaggtgaaa atgcatttct tcccagtgta tcagctgcca
     1201 gcactaagaa tgctcctgtt acagcggaat agggaagcag atgacggtaa tcgccaccaa
     1261 tgagcatacg tgccatatga ggcgctacca ggccaacaaa accaatgata ccggtgaaac
     1321 tgactacaga ggctgtaagc agagttaccg caccgccgca aagcagtctg gtccgcttta
     1381 catttatacc taaggctctt gctgtctcat ctccaccatc atctaacagg ttaaatgccc
     1441 atgcctgact cataaataca ggaaaagata ctgcaaagaa aaacagcatg atcccgatat
     1501 ctgaccatgt tacggctgta aggcttccaa aagaccagtg tacaatggcc ggaagctgct
     1561 gctcattcgc tataaactgc atggtggagt ttaaagcatt aaacaggtag tttaaggcaa
     1621 tacctgtaag aaccaatgct tccgggcgaa gctgtttgta acttgccact gtataaacaa
     1681 caatggcgca gatcacagac atgacaaacg cggccgctac agtagctccc ttctgcatcc
     1741 ataaaggaaa gccgccaaac aagatcataa gagccgctcc aaaagctgct gcactggaaa
     1801 gaccggtagt aaaggggctg gccatcgggt ttcctgtaat agcctgcata ccggaacctg
     1861 cgatccccag tccggctccc gcgatcacag ctgctgcaat tcttgggagc cttaacataa
     1921 ggagcacgtt ctgctctttc gcatctaaag gcgctactcc tttaaacatt ccgctaaaag
     1981 aagggagcca tgttacgatc atacggtctg gtgtcagctg ggctaccccc aggcaggcgg
     2041 cccatatagc tgctaccagt aacactaata gaagaacagc caggagaatg ttctttctcc
     2101 tggctcttcg cagataatca cctgctaaat ctcttttttc ctggttatca tccgccctca
     2161 tggtcattaa ccctgtgcag gtacgtgata gtaatgaggt gcgtttggtt caaatccctg
     2221 atacttcatc cactgagtga agattgcatc tgggtctaag tcggtcatca gatccggata
     2281 cagccatttt gccatgtaag cagcaccgat catctttcca aggccgccgc tcataaatgc
     2341 tgtattcaga tagaagtttc cttccttaac agcctttaaa tccttccagc ctggacgctg
     2401 cagcatttca tctgctttct ctgcaaactc ttcctgtgga ggtgctgtaa atacgccgct
     2461 gtttccgatt acattcttac cagcgtagat ctttacgatc aggtctggat cttcggttaa
     2521 gatctgctct ggatcaatgt ccttacctgc taaagaagca tcaccgaaaa tgttgatacc
     2581 gccagccatc aggatcatgt tgtgccagcc gtcattggaa gttccaggga tacaggtgct
     2641 gtaatcatcg ccgtactccc agtaaacttt tttacggtct gttacctgct ttaactgctc
     2701 attgatataa tcaactggtt tctggtagaa atcaaccagt tcttctgcct gctcctgctt
     2761 tccaaacagc tcgccgcaaa gacggatctg cttcggaaca tcagagttat cccaacctgt
     2821 gataacaact accttgatac caaaggactc taatttttca acagactctt ctactttacc
     2881 gttctttgga actaccagta cctgaggatc taatgcaacg atcttctcat aatcgtactc
     2941 atcttttcca aaggtctcat cttcgccaaa ctgtggccag tatacagggt cctgggaggt
     3001 gtactgatct acaccaacaa ctgcatcacc agcgcctacg gcacggatca gctcattatt
     3061 ataacggttt gcaacacaag cgcgggtgac cggaagttcc agttctactt ctcttccaag
     3121 gtcatctacg aatgtagttg tttccccttc ttttgcttct gcagtagttt cagctgcggt
     3181 tgtttcctcc gctgtagtct ctgctactgt tgtttcagct gctgtggtct cctgcttgga
     3241 agcagaacca gagcaaccag ttactgcgat cattgcgcct gctaagatcg ctgccagcca
     3301 tctcttgctg tttctcattt ttatgtctcc tttcagtctg ctatcctgtg tgtttccctt
     3361 ttactgtttt tatctattga tatacagctt tttgcacaag gataaaatat gctctccatc
     3421 ataaattgca taacagagcc atatcttccg caaaattaca aaattgcaca tttatatagt
     3481 gcatatgtat ttgcagactg aaaggtaata gtccctgtcc cagactattc ctctgtcaga
     3541 catccggccg gtctcctggc ttatggttca tcctccggac gtcttcccgc tttcgcagtg
     3601 acttttttgt cctttgtccc cgtttttaca gtagaggcag ctgcggggga ttttcacccc
     3661 acttccctga cggtctttca tttttctgta ccagaccgcc ttttttccag atgctgcaag
     3721 ttttcagttg agcctttaaa agggtatcac tttttgtctg atttcgcaac acctttttat
     3781 tacactgcaa tgtttgtatt tctatatacc tttctcctgt cgtttctgtc tctgcaaaag
     3841 aaggaataac ccggtacaga acacaaccga cataaaagaa ccacccgctg ttgccagtcc
     3901 cattggaaat aatgtttccg ggaatatctt agaggtaaga tacactcctg ggacacgcac
     3961 cagggcaatg gaggtaatat tgtgaataaa ggaatacccg gaccgtccac aggcacaaaa
     4021 ataaccactg aaactgaaat ggatccctgc aaacatacag tcccagatat aaccttttaa
     4081 atactgggca ccggcataga ccactgcctg gtcattggta aacaggttca ctacatttcc
     4141 tgctgtaaac tggatgataa tggcaatgat cagaccaaat cccccggcaa tacagatggc
     4201 atattttaaa gtagctgccg cccgctccgg ttttcctgct cctacattct gggcacctaa
     4261 agctgatact gttgataaca tggaagacgg cactaaaaac agaaagctga tcactttttc
     4321 cacaatacct actgctgccg catctgtaag acctctcctg tttgcaataa tagtgataat
     4381 gataaatgcc acctggatca gaccgtcctg catagcaata ggaaaaccga tctttaacag
     4441 tctgcccatg gtctcttttc tgggcttaaa atcgcttttc tttactgtaa tccctgtttt
     4501 tcccttcata aacatggtca gggccacgat cacactgaca gtctgggata aggtagttcc
     4561 cagcgctgct ccggaaggtc ctaaatgcaa tacgcccata aaaacgtaat ccagtgcaat
     4621 atttacaaca caggcaatgg cgataaaata cataggactc ttagagtctc ccattcccct
     4681 gaaaatagag ctgatgatgt tataagctgt aataaaagga atgccaagga aacatatctt
     4741 taaataaaga gcggttcctg ctagtgcctc ttccggcgta gacataatgg atacgattgc
     4801 gtttacggaa aggaataaaa ccaccgtcag aacaaccgat agtaccataa ataaagtagc
     4861 tgtatttcct gtatccgttg ctgctctttt cttatctctt gcgccgatgg cctgtccgat
     4921 gtttatagtt gctcccattg ccaggcctac gatcataaca gtaagcatat gcattacctg
     4981 gcttccaatg gaaacagctg tggtacttgc cacgccttca aactgaccaa tgatgaacag
     5041 atccgccatt ccatacaggg tttgaaggaa ataggatagt aaatacggaa gggaaaaata
     5101 gatgatgttt ttaaatacgc ttccttcagt aaggtttctt tccatgatat gtgtctctcc
     5161 tgtgttgtct tattgcaaat tgatctattt gcttgtatca gtttatactc tacaacagtt
     5221 gtagggtcaa gtgtaatgat ttaagaaaaa caaaaaacct gagcatttct gcccaggcaa
     5281 aatattattt atattataca taccttcaaa accacatatt gaactgttga ttttccgaat
     5341 acttacaact tt
//
LOCUS       DBMDUA010000184         5319 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5991_length_5319_cov_2.435608, whole genome shotgun sequence.
ACCESSION   DBMDUA010000184 DBMDUA010000000
VERSION     DBMDUA010000184.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5991_length_5319_cov_2.435608"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="ACZPCL_11390"
     CDS             <1..324
                     /locus_tag="ACZPCL_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163104.1"
                     /translation="HNDIVSVSFSVKDTADTGNFEIAKIEAEVDVSDMGPGTHPGTVT
                     FELGAAYELVSQDDLQIIVHDREPGDTVPAPTQEETGSSTRETTAAESSSGASNHTTA
                     AETSH"
     gene            384..647
                     /locus_tag="ACZPCL_11395"
     CDS             384..647
                     /locus_tag="ACZPCL_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1163105.1"
                     /translation="MVSGTTVIKNPSGLHLRPAGILCKEALLYKSKISFKVRNVTANA
                     KSVLSVLGACVKCGEEIEFNCTGPDEEKALAAMIKIVEDGLGE"
     gene            676..2397
                     /gene="ptsP"
                     /locus_tag="ACZPCL_11400"
     CDS             676..2397
                     /gene="ptsP"
                     /locus_tag="ACZPCL_11400"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR1163106.1"
                     /translation="MFKGTSASAGIGIGKAVIVEEAELVITRNEVADAEAEIQRFKGA
                     LEKTIAATQKMADDLASRVGEKEAEIMQGHMMLLSDPMLTGEIENSVKNDKVNCEFAI
                     ENVCNMYADMFASMGDELMQQRATDMRDIKVRMQRTLMGLESVDVSLLPKGSILVAKD
                     LTPSMTAGINPENVSGIVTELGGKTSHSAILARALEIPAVVAVNGLMDAVKDGDSVVL
                     DGSTGEVFVNPEASVEKEYEEKRSQFLKDKKELEKYIGQPSVTKDGVQVEIVANIGKP
                     EDVDKVLQYDGEGVGLFRTEFLFMDRTAMPTEEEQFEAYKKVAIALNGKPVIIRTLDI
                     GGDKEIPYMGLEKDENPFLGYRAIRLCLDRKEDIYKPQLRALLRASAYGNIRIMIPLV
                     TCIDELREAKALIEEIKAELDEKNIAYKKDIQVGIMVETAAASLIADIFAKEADFFSI
                     GTNDLTQYTMSVDRGNKKVSYLYSTFNPAVLRSIRHIIACGREAGIMVGMCGEAASDP
                     MMIPLLLAFGLNEFSMSASAILRARKMVTEYSVEELQAVADKAMSFATTAEVEDYMRK
                     FVEKITG"
     gene            2397..3704
                     /locus_tag="ACZPCL_11405"
     CDS             2397..3704
                     /locus_tag="ACZPCL_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HIR1163107.1"
                     /translation="MAVYLICYGISFILAWQHLYVLSGLVLVGAAVWLYIRDYIRTGN
                     LIHLRGLFCLFFVGGQGISCFKLSYLQTDWLLITWISFFLAVMAFWWVFEVLTRLYDG
                     WSAADMGSVYKYYSSADSPLQANRILISMAVITLVSILAFAFEAWRLGFVPMFSYGVP
                     HAYSYFHIKGIHYFTVSCVLVPSLSVVYCLMMARRGRGLVRYKGFWLAVICDIVAVMI
                     PLLCVSRFQLILAVGMAVFTYISMTGSFSLVYVGVLFVALIPAYVALTVLRSHSVEYL
                     NGIFEMKNSHMPIFITQPYMYIANNYDNFNCMAAELKHFSFGLKGLFPLWALTGLKFI
                     FPSLADFPLFVTKEELTTVTLFYDAFYDFGVVGMVFFGGLLGGVCYLLGRFRRKLTCP
                     AGHVIYAQIAMYMMLSFFTTWFSNPTTWFYLIVSGIVYVYVNS"
     gene            3911..5188
                     /locus_tag="ACZPCL_11410"
     CDS             3911..5188
                     /locus_tag="ACZPCL_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR1163108.1"
                     /translation="MEKKQSLDTICIQGGWQPKNGEPRQLPIYQSTTFKYTTSEQMGR
                     LFDLEENGYFYTRLANPTNDAVAAKICALEGGVGAMLTSSGQAANFYAIMNLCQAGDH
                     VVSSAAIYGGTTNLFTVTIPKMGVECTLVSPDATEEELAAAFRPNTKALFAETIANPA
                     LTVLDIEKFAKVAHEHGVPLIVDNTFATPINCRPFEWGADIVTHSTTKYMDGHAMTVG
                     GCIVDSGNFDWEAHHDKFTGLTEPDESYHGIIYTQKFGKLAYITKATSQLMRDLGSIQ
                     SPQNAFLINVGLETLHLRMPKHCENALKVATYLKSREDVAWVNYPGLEGDKYYELAKK
                     YMPNGTCGVISFGLKSGRKGAADFMDKLKLCSIETHVADSRTAVLHPASHTHRQLTDE
                     QLVEAGVDPSMIRLSVGIEGADDIIEDIRQALE"
BASE COUNT         1549 a         1047 c         1337 g         1386 t
ORIGIN      
        1 cataatgaca ttgtttccgt ctctttcagt gtaaaagata ccgcagatac ggggaatttt
       61 gaaatcgcaa agatagaggc agaggtagat gtaagcgata tgggacctgg aacccatcct
      121 ggaactgtta catttgaact gggtgcagct tatgaactgg taagtcagga tgatcttcag
      181 atcattgtcc atgacaggga accgggagat actgtaccgg cacctactca ggaagagaca
      241 ggaagcagta caagggagac aacagcagct gaaagcagct ccggtgcatc caatcataca
      301 acagcggctg aaacaagtca ttaattaagg ggaaaataag aaaaaaggca gtttatatgc
      361 tgcgggaaat caggagaata aatatggtaa gtggaacaac agtgatcaag aacccatccg
      421 gtcttcattt aagacctgca ggcatccttt gcaaagaggc tctgctttat aaatcaaaaa
      481 tctcttttaa ggtacgcaat gtgacagcaa atgcaaaaag tgtgctcagt gtgcttggag
      541 catgtgtaaa atgcggagaa gagatcgaat ttaactgtac cggccctgat gaggaaaaag
      601 ctcttgcagc aatgatcaaa attgtggagg acggattagg ggagtaaacc ataaattaat
      661 aaggaggaca cacatatgtt taagggaaca agtgcttctg ccggtattgg cattggcaaa
      721 gctgtgatcg tagaagaagc agaattagtg atcacaagaa atgaggtagc agatgcagag
      781 gctgagatcc agcgctttaa gggggctctg gaaaagacca ttgcagctac acagaagatg
      841 gcagacgatc tggcttcccg cgtgggagag aaggaagcag agattatgca gggacacatg
      901 atgctgttat cagatcccat gcttacaggg gagattgaaa atagcgtgaa aaatgacaag
      961 gtaaactgtg agtttgctat tgaaaatgtg tgcaatatgt atgcagatat gtttgcgtct
     1021 atgggagacg aactgatgca gcagcgcgct acggatatga gggatatcaa agtgcgtatg
     1081 cagcgtacat taatgggtct ggagtctgta gatgtatctt tactgccaaa gggaagcata
     1141 ctggtagcaa aggatctgac cccgtccatg acagcaggca tcaatccgga aaatgtttcc
     1201 ggtatcgtaa cagagctggg aggaaaaacc tcacacagcg ctattttagc aagggcactg
     1261 gagatcccgg cagtagttgc agtaaatggc ctgatggatg cagtaaagga cggagacagt
     1321 gtagtccttg acggaagcac cggagaggta tttgtaaatc cggaggcttc tgttgaaaaa
     1381 gaatacgaag aaaagagaag ccagttctta aaagacaaaa aagaactgga aaaatacatt
     1441 ggacagcctt ctgttacaaa agacggcgta caggtagaga tcgttgccaa tatcggtaaa
     1501 ccggaagatg tggacaaggt gttacagtac gatggagaag gcgttggtct gttccgtacc
     1561 gagttcctgt ttatggaccg tactgccatg cctacagaag aagagcagtt tgaagcatac
     1621 aagaaagtag ctattgcatt aaacggcaag ccggtgatca tccgtacatt agatatcggc
     1681 ggtgacaaag agatcccgta tatgggactg gaaaaggatg agaacccatt cctgggctat
     1741 cgtgccatcc gtctgtgcct ggacagaaaa gaggatattt acaagccaca gctgcgtgcc
     1801 cttttaagag ccagcgcata tggcaatatc cgtattatga tccctcttgt aacctgcatt
     1861 gacgagttaa gagaggcaaa agcactgatt gaagagatca aggctgaatt agatgaaaag
     1921 aacattgcat acaagaaaga catccaggta ggtatcatgg tagagacagc agccgcttcc
     1981 ctgatcgcag atatctttgc aaaagaggca gatttcttca gtatcggtac caatgacctg
     2041 acccagtata ccatgtcagt agacagagga aacaagaagg tatcctatct gtactctacc
     2101 ttcaatcctg cagtactcag aagcatccgc cacattatcg catgcggacg tgaagcaggc
     2161 attatggtag gtatgtgtgg tgaagcagcc agtgatccga tgatgatccc gctgttactt
     2221 gcctttggtt taaatgaatt cagtatgagc gcatccgcca tcctgcgcgc aagaaagatg
     2281 gtaacagaat acagtgtaga agaacttcag gcagtagctg acaaggctat gagctttgca
     2341 acaacagcag aagtagaaga ctacatgaga aagtttgtgg agaagattac aggttaatgg
     2401 cagtttatct tatttgttat ggaatcagtt ttattctggc ctggcagcac ttatatgtgc
     2461 tgtccggcct tgttttagtt ggagcagcag tatggctgta tatcagagat tatatccgga
     2521 caggaaacct gatccacctg cgaggtctgt tctgtctgtt ttttgtagga gggcagggaa
     2581 tttcctgctt taagctttcc tatctgcaga cggactggct gcttattaca tggattagct
     2641 tctttctggc agttatggca ttctggtggg tatttgaggt gctgaccaga ttgtatgacg
     2701 gctggagtgc agcggatatg ggttctgtat acaaatatta ttcatctgct gactcccctt
     2761 tacaggcaaa ccgtattctg atctccatgg cagtgatcac tctggtttct atcctggcct
     2821 ttgcttttga agcatggaga ctgggctttg tgccgatgtt ttcctatggc gtaccacatg
     2881 catattctta ttttcatatt aaagggatcc attactttac tgtatcctgc gtactggtcc
     2941 caagtctttc cgttgtatac tgtctgatga tggcaagaag aggaagagga ctggtaagat
     3001 acaagggctt ctggctggca gtgatctgtg atattgtggc agtgatgatc ccgctgttat
     3061 gcgtttcccg tttccagctg atccttgcag tgggaatggc tgtatttacc tacatttcca
     3121 tgacaggaag cttcagcctg gtttatgtgg gagtgttgtt tgtggcactg atcccggctt
     3181 atgttgcctt gactgtgctt cgcagccata gcgtcgagta cttaaacggc atttttgaga
     3241 tgaagaatag ccatatgccg atttttatca ctcagccata tatgtatatc gctaataact
     3301 atgacaactt taattgtatg gcagcggagt taaaacattt ctcctttgga cttaagggac
     3361 tgttcccgtt atgggcgctg acaggactta aatttatctt cccgtcactg gcagattttc
     3421 cactgtttgt gacaaaagaa gaactgacta cagtaacact gttttatgat gcgttttatg
     3481 attttggtgt tgtgggaatg gtatttttcg gcggtctttt agggggtgtc tgctatctgt
     3541 tgggacgttt cagaagaaaa ctcacctgcc ccgccggtca tgtgatctac gcccagatcg
     3601 ccatgtacat gatgttgtct ttctttacaa catggttttc caatcctact acctggtttt
     3661 acctgatcgt gtccgggatc gtgtatgtgt atgtgaattc ttaataatgg ataaaattga
     3721 aaaataaact gtttaggctc tgcggctgca gggccttttt gctgctgtac cattgactaa
     3781 cagaccaaaa cggttggttg aagcacctgt atgagtgatt tataatggaa tgagtgcaga
     3841 gaaaaataag cggctgcgta gcagacattt gtttttcgca agccattaaa ctcatacaag
     3901 gagaaggaac atggaaaaga aacaatcatt agataccatt tgtatccagg gcggctggca
     3961 gccgaaaaac ggagagccca gacagcttcc aatctatcag agtactactt ttaaatatac
     4021 tacaagcgaa cagatgggac gtctgtttga cttagaggaa aatggctatt tttatacaag
     4081 actggcaaat ccaaccaatg acgcagtagc agcaaagatc tgcgctctgg aaggtggcgt
     4141 aggcgctatg cttacctctt ccggtcaggc agctaacttc tatgcgatca tgaacttatg
     4201 ccaggcaggt gaccatgtag tcagctcggc agcaatctac ggcggaacca ccaacctgtt
     4261 tacggttacg atcccgaaaa tgggcgtaga atgcactctg gtaagcccgg atgcaacaga
     4321 agaagagctg gcagcagctt tccgtccaaa tacaaaggca ttatttgctg agaccattgc
     4381 aaacccggct ttaacagttc tggatattga gaagtttgca aaggtagctc atgagcatgg
     4441 tgtacctctg atcgtggaca ataccttcgc aaccccgatc aactgccgtc cgtttgagtg
     4501 gggcgcagat attgttaccc acagtaccac caagtacatg gatggacatg ctatgacagt
     4561 aggcggctgt atcgtagaca gtggaaactt tgactgggaa gcccatcatg ataagtttac
     4621 cggtttaaca gagccggatg agtcttatca tggcattatc tatacacaga agtttggaaa
     4681 actggcttat atcacaaagg caacttccca gctgatgcgt gatctgggat ccatccagtc
     4741 cccgcagaat gctttcctga tcaatgtagg actggaaaca ctgcaccttc gtatgccaaa
     4801 gcactgtgaa aatgccttaa aggtagctac ttacttaaaa tccagggaag atgttgcctg
     4861 ggtcaactat ccgggactgg agggagataa gtactacgag ctggcaaaga aatacatgcc
     4921 aaacggtacc tgcggcgtta tctcttttgg cttaaagtcc ggaagaaagg gcgcagcaga
     4981 ctttatggat aagttaaagc tgtgctccat tgagactcat gtggctgact cccgtacagc
     5041 tgttcttcat cctgcaagcc atacccacag acagctgaca gatgagcagt tagtagaagc
     5101 aggcgtagac ccgtctatga tccgccttag tgtaggtatt gaaggtgcag acgatattat
     5161 tgaggatatc cggcaggctc tggagtagac aaaatgaaag aaattcgtta ttttttgttg
     5221 agaatagcga ttaatcatta ataaaaacta cataaaattg tgcaatatgc acaaatacaa
     5281 taaaatattt ttgagaaatt tcaatctaga ctaaaaaaa
//
LOCUS       DBMDUA010000185         5318 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_5994_length_5318_cov_2.557408, whole genome shotgun sequence.
ACCESSION   DBMDUA010000185 DBMDUA010000000
VERSION     DBMDUA010000185.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5994_length_5318_cov_2.557408"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="ACZPCL_11415"
     CDS             complement(<1..1406)
                     /locus_tag="ACZPCL_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HIR1163109.1"
                     /translation="MNKKLEGHPISKVDPGSIGEELELEPGDRVLTINGNPVEDIFDY
                     EYYVNSPSMTMVVRKANGEEWELDIENDYEDLGLTFENGLMSDYRSCSNKCIFCFIDQ
                     MPPGMRETLYFKDDDTRLSFLQGNYVTLTNLKDKDVERIIRFKLAPINISVQTTNPEL
                     RCMMLHNRFAGRALKIIDRLYEAETPMNGQIVLCKGVNDGAELERSISDLSKYAPVMQ
                     SVSVVPVGLSKYRDGLYPLEPFTKEDACATIDMIERWQKKIYAEHGIHFIHASDEFYT
                     LAERELPEKERYDGYIQLENGVGMLRLMATEVADALDKVEGNDEPGFVSLATGRLPYP
                     YMEKYAGWIRKKFPNRTINIYPIRNDFFGERITVSGLITAKDLIAQLKDKNLGEYLLL
                     PINMFRIGETVFLDDLTAEDVEKALQVKIRIVKSDGQDFVHAILDPSSEPEPEPKEEG
                     LPGEEYGYEGYELDEIWE"
     gene            complement(1716..2903)
                     /locus_tag="ACZPCL_11420"
     CDS             complement(1716..2903)
                     /locus_tag="ACZPCL_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005539394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR1163110.1"
                     /translation="MANNFVFNDSLPVAPLKLAALESCKDFASKVDSHIVQFRRNDME
                     ELKRRKADLHYRGYDVDSYLLDFECPRFGTGEAKAVINESVRGTDIFVMADVMNYSIP
                     YTVCGYTNHMSPDDHFQDMKRVIGSCVATAHRVNVVMPFLYESRQHKRSKRESLDCAM
                     ALEELIQMGVENIITFDAHDPRVQNAIPLYGFDNFMPTYQFVKTLFTHDKTTQIDKDH
                     LMIISPDEGAMNRAVYLANNLGVDMGMFYKRRDYSKVVNGRNPIVAHEFLGSSVEGKT
                     VLIIDDMISSGESMLDTCKELKERKAAKAIVCCTFGLFTNGLEKFDEYYEKGYLDYVI
                     TTNLNYRPKELFDRKWYLEADMSKYTAAIINSLNHDRSISASLSPTDKIQKLVQRRHN
                     GEI"
     gene            complement(3028..3879)
                     /gene="pgeF"
                     /locus_tag="ACZPCL_11425"
     CDS             complement(3028..3879)
                     /gene="pgeF"
                     /locus_tag="ACZPCL_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIR1163111.1"
                     /translation="MIPWNYKNEKNIFIQREENGVPYLSFAALEETGLVVNGFSTRLG
                     GASKGKYATMNFAWNKGDDPADVLENYTRMAKALGVDRDRMVASQQTHTTNVRLVTEE
                     DAGKGVVRERDYTDVDGLITNVPDLTLATFYADCVPLYFVDPKHKAIGLSHSGWRGTV
                     NRMGQCTIDAMKKAFGTSPKDLITCIGPSICQDCFEVGEEVAEAFMESFKPEWHNEII
                     APGKRPDKYQLDLWRANEIIFMEAGVKPENIHTTNICTMCNHEYLFSHRKVGNERGNM
                     GAFLCLR"
     gene            complement(3937..4278)
                     /locus_tag="ACZPCL_11430"
     CDS             complement(3937..4278)
                     /locus_tag="ACZPCL_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR1163112.1"
                     /translation="MNKRKIGSRYEEAAAAFLQKQGFRILGKNFRCRQGEIDLVCREG
                     KELVFTEVKYRSDASCGSPFEAVDFRKQEKIRRTALFYLCRYGYPENTPCRFDVVGIT
                     GSNIQLIRNAF"
     gene            complement(4299..4946)
                     /locus_tag="ACZPCL_11435"
     CDS             complement(4299..4946)
                     /locus_tag="ACZPCL_11435"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR1163113.1"
                     /translation="MARELKGEKLEKELLRLEEMKAYEKENAQYELICGIDEAGRGPL
                     AGPVAAGAVILPKDCQILYLNDSKKLSEKRREELFLEIKEKALAWSVGIATPQRIDEI
                     NILQATYEAMREAIKGLGVQPGLLLNDAVTIPGIEIPQIPIVKGDAKSVSIAAASILA
                     KVTRDHMMMEYDEIYPGYGFGKHKGYGTKAHIEALKTLGPCPIHRGSFIGHFVEV"
     gene            complement(4947..>5318)
                     /locus_tag="ACZPCL_11440"
     CDS             complement(4947..>5318)
                     /locus_tag="ACZPCL_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlqF/YawG family GTPase"
                     /protein_id="HIR1163114.1"
                     /translation="ELLDTPGILWPRFEDQQVGLHLALIGSINDQILNKDELACELIS
                     LLEKDYPQVVKDRFGIENVEEDRVKVLEEIARSRACLMKGGELDLARASALLLDDFRA
                     GKLGRISLEKAEDYKDRIKVK"
BASE COUNT         1323 a         1331 c         1053 g         1611 t
ORIGIN      
        1 tcttcccata tctcatctaa ctcatagcct tcataaccat attcttctcc aggaagccct
       61 tcttcctttg gttcaggctc cggctctgaa gatggatcaa ggatagcgtg gacaaaatct
      121 tgcccgtctg attttactat acgtatctta acttgtaaag ccttttctac atcttctgct
      181 gttaagtcat ctaagaatac agtttcaccg atacggaaca tattaatagg aagaaggaga
      241 tattctccca gatttttatc cttcagctgg gcgatcaggt cttttgcagt gatcagacct
      301 gaaaccgtaa tacgctcccc aaaaaaatca ttccggatgg gataaatgtt gatggtcctg
      361 tttgggaatt ttttccggat ccatccagca tatttttcca tataaggata aggaagacgg
      421 ccggttgcaa gggatacaaa gcccggctca tcatttcctt ctactttgtc cagcgcgtcc
      481 gctacttctg tagccataag acgcagcatt cccacgccat tttccagctg gatatagccg
      541 tcatagcgtt ctttctccgg cagttccctt tctgccagtg tataaaactc atcgctggca
      601 tggataaaat ggatcccgtg ttctgcgtag atcttcttct gccaccgttc gatcatatca
      661 atagtagcgc aggcgtcttc ttttgtgaaa ggctctaatg gatataagcc gtctctgtat
      721 ttggaaagtc ctacaggaac tacggaaacg ctctgcataa caggggcata tttggacagg
      781 tcactgatgg aacgttccag ctctgccccg tcatttactc ccttgcagag aacgatctgg
      841 ccgttcatag gagtttccgc ttcatataag cggtcaatga tctttaaggc ccgtcccgca
      901 aaccggttgt gcagcatcat acaccgcagt tccggattgg tcgtctgtac ggaaatgttg
      961 atgggcgcca gtttgaagcg gatgatacgc tctacatctt tatcttttaa gttggtcagt
     1021 gtaacataat ttccctgaag gaacgataaa cgggtatcat catctttgaa ataaagggtc
     1081 tctctcattc ccggcggcat ctggtcgata aagcagaaaa tacatttgtt gctgcaggaa
     1141 cggtaatcgc tcatcagacc attttcaaaa gtcagtccca gatcttcata atcattttct
     1201 atatccagtt cccattcttc cccgttggcc tttcgcacca ccatagtcat agacgggctg
     1261 tttacataat attcgtagtc aaaaatgtct tcaacgggat ttccgttgat ggtaaggacc
     1321 ctgtctcccg gctccagctc cagttcctct ccaatggaac ccggatctac ttttgagatc
     1381 ggatgtcctt ccagtttttt attcatattc atataacctc tgtgtatttc gttagttcac
     1441 ttaggttcac gcactgacgc gctccactgc ctgctgtcgc aggcagaacc ttcgttaatg
     1501 gctcgtgaaa aataaatgcc acctttcggc gtcgcttatt tttcccttcg ctcattacat
     1561 cataacaatt ttagaaagtt ttgtaaacca gatttacagc acgaaaatgc cgcaaagcag
     1621 atttaccctc tgttttgcgg catgatgatt gctagtatat acactcacga cccccgcgct
     1681 atcgcgctcc actgcctgct gtcgcagatg ggtcgttaga tctccccatt gtggcgtctc
     1741 tgtaccagct tctggatctt gtctgttgga gacagggatg cgctgatgga acggtcgtgg
     1801 tttaaggagt tgatgattgc tgcagtgtac ttgctcatat cagcttccag ataccacttg
     1861 cggtcgaaaa gttcttttgg acggtaattt aagttggtgg taattacata gtccagatat
     1921 cccttttcat aatattcgtc aaacttctca agaccattgg tgaacagacc aaaggtgcag
     1981 catacgatcg cctttgctgc ctttctctcc tttaattcct tacaggtgtc taacatgctc
     2041 tcaccggagg agatcatatc gtcgatgata agaacggtct ttccttctac agaagaacca
     2101 aggaattcat gtgcaacgat cgggttacgt ccgtttacta ccttggagta gtcacggcgt
     2161 ttatagaaca tacccatatc aactcccagg ttgtttgcca ggtatacagc acggttcata
     2221 gcgccttcat ccgggctgat gatcatcaga tgatctttgt cgatctgagt ggtcttatca
     2281 tgagtaaaca gtgtctttac aaactgataa gttggcatga agttgtcaaa tccgtacagc
     2341 gggatggcat tctgtactct tgggtcatga gcatcaaaag taataatgtt ttcaacgccc
     2401 atctggatca actcttctaa tgccatagca cagtctaagg attcacgttt gcttctctta
     2461 tgctgacggc tttcatacaa gaaaggcata actacattta cgcggtgcgc agttgccacg
     2521 caggaaccga tcacacgctt catatcctgg aaatggtcat ctggtgacat gtgattggtg
     2581 tagccgcaaa cagtgtaagg aatgctgtag ttcattacat ctgccattac aaaaatgtct
     2641 gtacctctga cagactcatt gatcactgcc tttgcctcac cggtaccaaa tctcgggcac
     2701 tcaaaatcca acagataaga gtccacatca taaccgcgat aatgcagatc tgccttgcga
     2761 cgctttaatt cctccatatc gttgcggcgg aactgaacga tgtgagaatc taccttagat
     2821 gcaaagtcct tgcagctttc cagtgcagct aactttaaag gtgctactgg aagagagtca
     2881 ttgaatacaa aattattagc catgaaagca tactcctttg tcctataatg gtcctaatag
     2941 tcctaataat agtcctttat cctattgttt tataccattt tccacgataa gggtcaagta
     3001 ttccaggaca tttccttaaa aaatacatca cctaaggcac agaaaagcac ccatattgcc
     3061 tctttcattt ccaactttac ggtgggaaaa gagatattca tggttgcaca tggtgcagat
     3121 attggtagtg tggatatttt ccggctttac cccggcttcc ataaaaatga tctcattggc
     3181 ccgccacaga tctaattggt atttgtccgg acgttttcca ggggcaatga tctcattgtg
     3241 ccactccggc ttaaaacttt ccataaatgc ctctgccact tcttctccta cttcaaaaca
     3301 gtcctggcag atgcttggcc cgatgcaggt gatcagatcc ttagggctgg ttccaaaggc
     3361 ttttttcatg gcatctatgg tacattgtcc cattctgttt acagtgcccc gccaaccgga
     3421 atgggaaagg ccgatggctt tatgtttcgg atccacaaaa tacaaaggca cacagtctgc
     3481 ataaaaggtt gccagtgtaa gatcaggcac attggtgatc agtccgtcca catctgtgta
     3541 atcccgttct ctcactacac cttttccagc gtcttcttct gtcacaaggc gcacattggt
     3601 ggtatgggtc tgctgggatg ccaccatacg gtccctgtca actcccaatg cctttgccat
     3661 acgggtgtag ttttccagca catcagccgg atcatctcct ttattccagg caaagttcat
     3721 agtagcatat tttcccttgc ttgcccctcc taaacgggtg gaaaatccat ttaccacaag
     3781 gcctgtctct tccaaggcag caaaagaaag atagggaacc ccattttctt ccctttgtat
     3841 aaatatattc ttctcatttt tatagttcca tgggatcatc tgtctgcctc ttttctatat
     3901 aaatcaatat aaagatcctt tacaaggata ctttgtttaa aaagcatttc gtatcagctg
     3961 tatattactt ccggttattc ccaccacgtc aaaacggcag ggcgtatttt ccggatagcc
     4021 gtagcggcac agataaaaca gggctgtgcg ccgtattttc tcctgcttgc gaaaatctac
     4081 tgcctcaaaa ggtgaaccgc aggatgcatc ggacctgtat tttacctctg taaataccag
     4141 ttcttttcct tccctgcaca ccagatcgat ctccccctgt ctgcagcgga agtttttccc
     4201 taaaatgcga aatccctgtt tttgcaaaaa agcagccgct gcttcttcgt aacggctgcc
     4261 aatttttctt ttattcatca aatcttttat attttcccct atacttcaac aaaatggccg
     4321 ataaagcttc ctctgtggat aggacatggg ccaagtgtct ttaatgcctc gatatgtgct
     4381 tttgtgccat atcctttgtg ttttccaaag ccatagcctg gatagatctc atcatattcc
     4441 atcatcatat ggtccctggt aactttggct aaaatgctgg ctgctgcaat agatacactt
     4501 tttgcgtcgc cttttacaat agggatctga ggaatttcaa tcccgggaat ggtcacagcg
     4561 tcatttaaca aaagtcccgg ctgtacgcca agtcctttga ttgcctcacg catagcctca
     4621 taggtagcct gcaggatatt gatttcatcg atgcgctgcg gtgtggcaat tcctacgctc
     4681 caggcaagag ccttttcctt gatctccaag aacaactctt ccctgcgctt ttcagaaagc
     4741 tttttagagt cattcagata taagatctga cagtcctttg gaaggataac agcccctgcc
     4801 gctaccggac ctgctaaagg tcctcttccg gcttcgtcaa taccacagat caactcatac
     4861 tgggcatttt ctttttcata ggctttcatt tcttccagcc ttaaaagctc tttttccagt
     4921 ttttcccctt ttaactctct ggccatttat ttcaccttga tcctgtcttt gtaatcctct
     4981 gccttttcca ggctgatacg acctaatttg cctgcgcgga aatcatctaa aagcaatgca
     5041 gaagctctcg caagatctaa ttccccgcct ttcatcaggc aggcacggct tcttgcgatc
     5101 tcttctaata ctttcacacg gtcctcttcc acattttcaa tgccaaagcg gtcttttacc
     5161 acctgtggat aatctttttc caaaaggctg atcagttcac aagccagttc gtccttattt
     5221 aagatctgat cattaataga accaatgaga gccaggtgca gacctacctg ctggtcctca
     5281 aaacgtggcc ataagatacc aggggtatct aacagctc
//
LOCUS       DBMDUA010000186         5284 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6031_length_5284_cov_2.473857, whole genome shotgun sequence.
ACCESSION   DBMDUA010000186 DBMDUA010000000
VERSION     DBMDUA010000186.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6031_length_5284_cov_2.473857"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1573
                     /locus_tag="ACZPCL_11445"
     CDS             <1..1573
                     /locus_tag="ACZPCL_11445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016289.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HIR1163115.1"
                     /translation="TETFPAYLSNPVKSVLTVDGKEIPSRPRSFSESTGKSLHVPLVY
                     DPGTREAVSMDRQKQFLENVSGKLVLGYGYDERYAKILEQYGAAGWIQIWTSGEDVIH
                     EDTVSPVWGTPDMDSSLFQLKMPVVAISGPTGEYLIQKLENARKNGQILYADLDSRVD
                     TGVRSVQLPIADIPGRKKDFVLLSCHYDTWYRGAFDNCTANALALELAKYFQDRKEQL
                     AYSLKIAWWPGHSNGRYMGSTWYCDHHWDELYENCIAHVNLDLLGSKGADHTLAIRTA
                     GLEGTKWLKEHVMEADPLAEIQIGRIGRGADQSFWGAEIPYHINPRYEARKERKQSDA
                     PGPGVYWWHTAEDTFDKIDFDGLMRDGAVVRSLLCGLLNEEMLPADFSEYFHTWNGYL
                     EPLKNSSKYGEEIEKIQKQLKTVIQLCVDLEKKWNTEEQEKGEPIHLEDHNRLCRITG
                     GVISRLMHSSGSAYEQDTSFAYGPLHLLGASAKGDFQKGPKDWELFYHTTFIRQRNRM
                     VTELRKLQKQIEQEF"
     gene            1587..2774
                     /locus_tag="ACZPCL_11450"
     CDS             1587..2774
                     /locus_tag="ACZPCL_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR1163116.1"
                     /translation="MKKEVIKQAVDQYLPELVKIADEIYDLAEPGMEEVRSAGLLTDY
                     LEKMGFQVEKGIAGLPTAFRAVYEQGQGGPSFGLLAEYDALKDIGHACGHHMQGPSVI
                     GAALALRDTCKDQPYKIVIYGTPAEETIGGKIIMQEKGCFKDIDIALMMHAAPSTCVD
                     LRCMALECFYVTFHGVESHAAMSPHKGKSAFDAALLSFQAIEFMREHVLEDSRMHYTI
                     LDAGGPSNIVPGRAKAEYTLRSYSTDYLENVIVPRFKDIIKGACLMTGTTCDLERSYP
                     FKAKIPCVTLNDLIMENAHFFQAPQITGPREKTGSTDFGNVMYEVPGCCIRTAFVPEG
                     TAAHSKEYLEAGKNQKAHEALRSGSEILAGTCMDILEHPEFLQKMKEEFEERKRKEQM
                     ETE"
     gene            2845..3048
                     /locus_tag="ACZPCL_11455"
     CDS             2845..3048
                     /locus_tag="ACZPCL_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1163117.1"
                     /translation="MVTVSGLEIDEHQQVLNRDFEPIPGLYATGNCSGRRFGVQYTTS
                     LPGQSIGIAQTLGRELGLYLTGV"
     gene            complement(3206..5134)
                     /locus_tag="ACZPCL_11460"
     CDS             complement(3206..5134)
                     /locus_tag="ACZPCL_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012927.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR1163118.1"
                     /translation="MENKYYPHLFQKGTVGGVVLKNRIVRNSMGTYLGNPDGTVTDRQ
                     IKAYAQAADGGAGLIFMDNAVPVPMVSCGLRADKDEFIAGLTLLAETVKEHGAVPGIQ
                     LAHPGRDAAFVGSADVIGASEITFEPWYEMGYKMPRALTIEEIHDLVERFGDAAVRCK
                     KAGFEIIEIHGAAGCIPTNFLSPHDNKRTDMYGGSLHNRMRLLLEIVRNIKKKCGPNF
                     PVGVKLSTEDWEPEGIRIEETIEVAKALEKEGVSHLNIMGGTHATASREFLLPNAFNA
                     KHTKMLKDAVNIPVFIGHNIFSPEDAEKMLAEGNADFVALGRSQLAEPAWAKKAKEGR
                     ASDIKPCINCLIGCIDRGMLSHTPIHCTVNPSLYRFECKPVEKAETRKQVAVVGAGPA
                     GCEAALTASKRGHQVTIYEKRCIGGAMIEASKPENKANIKRLIHYYEDHIMHDPNITL
                     VKEEVEYETLVHSGYDAVIIAVGGKTRILNVPGIEKNSVIYASDYLNGSTAVTGNTVV
                     VIGGGITGAETALELNAEGKKVTIVEMTDAFLANFSSACQAYLATINETDIKILTGKR
                     LTAVHENNVILTDRWGNETTVPADSVVIAAGFTRQNELSDKLEENTDMQVFNVGDSKR
                     VRQIYDAIHEGFIAARQI"
BASE COUNT         1490 a         1128 c         1238 g         1428 t
ORIGIN      
        1 tacagaaaca tttcctgctt atttaagtaa tccggtaaaa agtgttttga ccgtagatgg
       61 aaaagaaatt cccagtcgtc ccagatcttt ttctgaaagt acaggaaaat cccttcatgt
      121 tcccctggtg tatgatcctg gaaccagaga agcagtttct atggacagac agaagcagtt
      181 tttagaaaat gtttccggaa agctggtgtt gggatatggt tatgatgagc ggtatgcaaa
      241 aatactggaa cagtatggag ctgccggatg gatccagatc tggacttccg gagaagatgt
      301 gatccatgag gatacagtca gtcctgtctg gggaacaccg gatatggact cgtccctgtt
      361 tcagcttaag atgccggtgg tagcaatatc tggtccgaca ggagagtatc tgatccagaa
      421 actggaaaat gcccggaaaa atggacagat cctctatgcc gatctggaca gccgggtaga
      481 tacgggagta cgcagcgtgc agcttcccat agcagatatt ccaggcagaa aaaaggattt
      541 tgtcctgctt tcctgccatt atgatacctg gtacagaggt gcttttgaca actgtactgc
      601 caatgcactg gctctggaac ttgccaaata tttccaagat aggaaggaac agcttgccta
      661 ttctttaaaa attgcctggt ggccggggca ttccaatgga cggtatatgg gttcaacctg
      721 gtactgtgat caccactggg atgaattata tgaaaactgt attgcccatg taaatctgga
      781 tcttcttggt tcaaaaggcg cggaccatac ccttgccatc cggaccgctg gtttggaagg
      841 aacaaaatgg ctgaaagaac atgtaatgga agcagatcca ctggcagaga tccagatcgg
      901 acggattggc cgcggtgcgg atcagtcttt ctggggagca gagatccctt atcatatcaa
      961 tccaagatat gaagcaagaa aagaaagaaa acagtcagat gcaccgggac ctggtgtgta
     1021 ctggtggcat acagcggaag atacctttga caagatcgat tttgatggtt tgatgcggga
     1081 tggggcagtg gttcgttctc tcttatgcgg acttttaaat gaagaaatgc ttcctgcaga
     1141 tttttctgaa tactttcata catggaatgg gtatctggaa ccgttaaaaa attcttctaa
     1201 gtatggagaa gaaattgaga aaatacagaa gcagttaaaa acagtgatcc agttatgcgt
     1261 ggatctggag aaaaaatgga acacagaaga acaggagaaa ggtgaaccaa tacacttaga
     1321 agatcacaac cgcctctgca ggataacagg cggagtaatc agccgtctca tgcattcttc
     1381 cggatctgca tatgaacagg atacctcctt tgcatacggt ccgttacatt tacttggcgc
     1441 atcagcaaaa ggagactttc aaaaaggtcc gaaagactgg gaactgtttt accataccac
     1501 atttatacgc cagagaaatc gaatggtaac ggaactgaga aaattacaga aacaaataga
     1561 acaggaattt taacaggagt atatacatga aaaaagaagt cataaaacag gcggttgacc
     1621 agtatttgcc ggaactggtc aaaatagcag atgaaatata tgatcttgca gaaccgggaa
     1681 tggaagaggt aagatcagcc ggccttttga ctgattatct ggaaaaaatg ggatttcagg
     1741 tagaaaaagg gatcgcagga cttccaaccg catttcgggc tgtttatgag cagggacagg
     1801 gaggaccatc ttttggcctt ctggcagaat atgatgccct gaaagatatt ggacatgcat
     1861 gcggacacca tatgcagggg ccatctgtga tcggtgctgc cctggcacta cgcgatacct
     1921 gcaaagacca gccttataag atcgttatat atggtacacc tgcagaagag accattggcg
     1981 gcaagatcat tatgcaggaa aagggctgtt ttaaggatat agatattgca ctgatgatgc
     2041 atgcagcgcc ttcaacctgt gttgatctgc gatgcatggc actggagtgc ttctatgtga
     2101 cttttcacgg ggtagaaagc catgcagcta tgagcccaca taagggaaag agtgctttcg
     2161 acgcagctct tctctctttc caggccattg aatttatgcg tgagcatgta ttagaagact
     2221 cacggatgca ttatacaatt ctggatgcag gcggtccatc caatattgtt ccgggacggg
     2281 caaaagcaga gtatacactg cgttcttaca gcaccgatta tctggaaaat gtgatcgttc
     2341 cccggtttaa agatattatc aaaggtgcat gcctgatgac gggaaccacc tgcgatcttg
     2401 agcgcagcta tccgtttaag gcaaagatcc cctgtgtaac attaaatgac ctgatcatgg
     2461 aaaatgccca tttcttccag gccccccaga tcacaggccc aagagaaaaa acaggttcca
     2521 ccgattttgg aaatgtcatg tatgaagtac caggctgctg tatccgcact gcctttgtac
     2581 cagaaggaac tgcagcccat tccaaagaat atctggaagc cggaaaaaac caaaaagccc
     2641 acgaagccct gcgctccggc tctgagatct tagccggaac ctgtatggat attctggaac
     2701 acccggaatt tcttcagaaa atgaaagaag aatttgaaga aagaaagcgg aaagagcaga
     2761 tggagacaga gtaagatttc ctaaaagaag tgaagcggta taataaaaga cagccacaga
     2821 ccaggtggcc agtctttaaa aatactggta acagtcagcg gactggagat cgatgaacat
     2881 cagcaggtct taaaccgtga ttttgaacca attcccggtt tatatgctac aggtaactgc
     2941 agtggaaggc gatttggtgt acagtataca acatcactgc ctggacaaag cattggaatt
     3001 gcccagactc ttggaaggga gttaggactg tacctgacgg gggtatagct ggcagctttg
     3061 tctatacgga aaagaaagat aataaaccta aaaaagaaaa ggaaacctga ataaatcgtg
     3121 aaaaagatat cgaccgccgg aatattcatc cgacggtcgt ttatagttgg atttgatcaa
     3181 ggaagacaga gtgggtgcaa tggtgttaaa tctgtcttgc agcaataaat ccttcatgga
     3241 ttgcgtcata gatctgacga acgcgtttgc tgtctccaac attgaatacc tgcatatctg
     3301 tattttcttc cagtttatcg ctcagttcat tctggcgggt aaaaccggcg gcgatgacta
     3361 cactgtcagc cggaaccgtt gtttcattac cccatcgatc ggtcagaatg acattgtttt
     3421 catggacagc ggtcaggcgt ttgccagtca ggatctttat gtctgtttca ttgatcgttg
     3481 caagatatgc ctgacaagcg gaactgaaat tcgcaagaaa agcatccgtc atttctacga
     3541 tagtaacttt tttgccttcg gcatttaatt caagcgcggt ttccgcaccg gtgatgccgc
     3601 ctccgatcac aactacagta tttcctgtta ctgctgtgga gccatttaaa taatcactgg
     3661 cataaatgac ggagtttttc tcaattccag gtacatttaa tattctggtt tttccgccga
     3721 cagcaatgat aactgcatca taaccgctat gaacgagagt ctcatattca acttcttctt
     3781 ttaccagtgt aatgttcgga tcatgcataa tatgatcttc ataatagtgg atcaaccgtt
     3841 tgatattggc tttgttttcc ggtttgctgg cttcaatcat cgctccgccg atgcatcgct
     3901 tttcatagat tgtaacctga tgtccgcgtt ttgaagcagt aagagcggct tcacagcctg
     3961 caggaccggc tccgactact gcaacctgtt tcctggtttc tgctttttca acaggtttgc
     4021 attcaaaacg gtataaggat ggatttacgg tacaatggat cggtgtgtgg ctcaacattc
     4081 ccctgtcgat acatccgatc agacagttga tacaaggttt aatatcgctg gctctgcctt
     4141 cttttgcttt ttttgcccat gccggctctg ccagctgtga acgacctaaa gcgacaaaat
     4201 cggcatttcc ttcagcgagc atcttttcag catcttctgg agagaaaata ttatgaccga
     4261 tgaaaacagg aatgtttact gcatctttca gcatttttgt gtgtttggcg ttaaatgcat
     4321 ttggcagcag gaattctctg gaagctgttg catgggttcc acccatgata ttcagatgag
     4381 atactccttc tttttccaat gcctttgcta cctcaatggt ttcttcaata cgtattcctt
     4441 ctggctccca gtcttcggta cttaatttga cacctaccgg gaaatttgga ccgcatttct
     4501 ttttgatgtt tctgacgatt tccaggagca gacgcatgcg gttgtgcaga gagccaccat
     4561 acatatcagt acgtttatta tcgtggggag acaggaaatt ggtcggaata catccggcag
     4621 caccgtggat ttcgataatt tcaaaaccag cttttttaca tcgtactgcg gcgtcaccga
     4681 aacgttcaac cagatcatga atttcttcta ttgtcagggc tcttggcatc ttatagccca
     4741 tttcatacca tggctcaaaa gtgatttcag atgcaccaat gacatctgcg ctgccgacaa
     4801 aagcagcatc tcggcccgga tgagccagct gaatgccggg aacggcccca tgttctttta
     4861 ctgtttcagc aagaagggta agacctgcga tgaattcgtc cttgtctgcc cgtaagccgc
     4921 aggataccat tggaaccggg actgcgttat ccataaagat aagtccggca ccgccatctg
     4981 ccgcctgggc ataagctttg atctgacggt cggttacggt tccgtcagga tttccaagat
     5041 aagttcccat agagttgcga acaattcggt ttttcaggac aacaccgcca acagttcctt
     5101 tttgaaataa atgtggataa tacttgtttt ccatattatc ttttcctttc acgtaaattt
     5161 ttgatataat cccatctttg acggtcaaat ttatcaaaac accgctgaaa gccttattct
     5221 acctgggttt ttcagcggtg ccttttcgtt ttactatata gtcttttttc tgaatcagat
     5281 atgg
//
LOCUS       DBMDUA010000187         5212 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6108_length_5212_cov_2.618470, whole genome shotgun sequence.
ACCESSION   DBMDUA010000187 DBMDUA010000000
VERSION     DBMDUA010000187.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6108_length_5212_cov_2.618470"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(92..919)
                     /locus_tag="ACZPCL_11465"
     CDS             complement(92..919)
                     /locus_tag="ACZPCL_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1163119.1"
                     /translation="MYRVLLADDEQIERMALARRLMRRFGDILQISEATNGKEAVQLY
                     EKEHSQIIIMDISMPELNGVEAAEKIRSMDEDCIIVFLTAYDEFSYAKRAIVIRALDY
                     LLKPCDEEELTAVMEEAMRLTDKAVENKKNSSGSGEEKTEAEKEAEKRKEQWPWNPDA
                     EPPKDPETERVNQVAEKIRTYIRENYMNEISMQDAARQFNYSDAYFCKLFKQCFDQNF
                     TTYLTNFRINEAKRLLKDRNISVKDAGMKVGYYDSNYFAKVFKRVTGMIPSEYREAN"
     gene            complement(981..2411)
                     /locus_tag="ACZPCL_11470"
     CDS             complement(981..2411)
                     /locus_tag="ACZPCL_11470"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1163120.1"
                     /translation="MKKWFCLPLQEKLKYLTFSVGAVVFLSIAANMLVAFFGLGGFGN
                     ILKSNSRSLAFWTAMDEESAAFDRFIQEQTKETRQEWEKSAKHTELMLEELPYKYKEI
                     GKERYARTWTLHNLYENYDRFRRELMEKETIGEADMQEVYRLRRIQGYLAGQAGQLEQ
                     LTVRNGSSQYEKQRYMLVVIPVCSLICGMAAFWMVRQMNRTASRYIVKPIVELAADSV
                     RIGDNDFTGPDVVAEGEDEISRLVQEFCRMKASTRENIRNMEEKHAMEQKLNDMRLQM
                     LKSQINPHFLFNTLNMIASTAQIEDAVATEKMITALSRLFRYNLKSAGTVMPLERELK
                     IIQDYMYLQKMRFGQRVRYDTDCAPETLDVLVPSFVLQPLVENSIKHGLSKESKGGRI
                     FIRTWMRGERLWISVSDTGVGMEEERLQQIRAALNDGTENEIGIGVGNIYRRVHGLYE
                     DGEMFIYSKKGCGTAVQLAFTPKDIV"
     gene            complement(2545..2763)
                     /locus_tag="ACZPCL_11475"
     CDS             complement(2545..2763)
                     /locus_tag="ACZPCL_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1163121.1"
                     /translation="MIDYSPFWKTLEISEENWYTLTKKYHMSSSTLYRLKHNQDISMK
                     TVNDLCRILGCQIEDIATYVPSDKDQPL"
     gene            2893..3090
                     /locus_tag="ACZPCL_11480"
     CDS             2893..3090
                     /locus_tag="ACZPCL_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163122.1"
                     /translation="MSWYTEEEIEKLLEDEELRKRIARFVTMSGEEFFDEVYTHLSPE
                     ELEEYLEENPSERKYLKRYEP"
     gene            complement(3123..3830)
                     /locus_tag="ACZPCL_11485"
     CDS             complement(3123..3830)
                     /locus_tag="ACZPCL_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1163123.1"
                     /translation="MNEMNFKNRGEQVFETLKREILDLKLEPGRMISENEICERFGVS
                     RTPVRDALRLLQEQGFVETVPYRGTYVTLLSLDNIKQMIYMRVAVETMVLRDFLKVQN
                     PMTLEEIRHAIAMQKALIQTPGFEPEQFYHMDAELHSIWFGAVRRQKLWDMLQAQQLH
                     YTRFRMLDFTTETDFTRITGEHEEIFRLICEKDEKGLEKALKDHLYFSMKRMRHAIEV
                     DYKDYFEEEPEEGRFVI"
     gene            complement(3852..4658)
                     /locus_tag="ACZPCL_11490"
     CDS             complement(3852..4658)
                     /locus_tag="ACZPCL_11490"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HIR1163124.1"
                     /translation="MADYLNNFSLEGKVALITGASYGIGFAIAKAMAGAGATIVFNDI
                     KQELVDKGLAAYEAEGVKAHGYVCDVTNEEAVNALVAQIEKEVGVIDILVNNAGIIKR
                     IPMCEMSAAEFRQVIDVDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGEYNIQCNGIGPGYIATPQTAPLREIQPDGSRHPFDQFI
                     VSKTPAGRWGNAEDLGGPAVFLASDASNFVNGLVLYVDGGILAYIGKQPK"
     gene            complement(4684..>5212)
                     /gene="kduI"
                     /locus_tag="ACZPCL_11495"
     CDS             complement(4684..>5212)
                     /gene="kduI"
                     /locus_tag="ACZPCL_11495"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HIR1163125.1"
                     /translation="LYITKGTKEVYFESEDASKPAKFYMVSAPAHTSYTTTFIPIEKA
                     AKRPCGEAALSNKRVINQFIHPDVLKTCQLSMGMTVLEPGSVWNTMPAHTHERRMEVY
                     MYFEVPGDNVVFHMMGEPIETRHIVMKNEEAVISPSWSIHSGAGTSNYTFIWAMGGEN
                     MEFDDMDTMKPNEMM"
BASE COUNT         1317 a         1308 c          969 g         1618 t
ORIGIN      
        1 ttgaggtgga aaatcttgtc caaaaatacg tacaatgaca actttgtcct gttttgtatt
       61 agttttatat tggttttcta tttgccttgt atcaattagc ttctctgtat tcagacggga
      121 tcatgcctgt cacacgctta aagactttgg caaaatagtt ggagtcataa tatcccacct
      181 tcatgcctgc atctttcaca ctgatatttc tgtcttttaa cagtcgtttc gcctcattga
      241 tgcggaaatt cgtaagataa gtagtaaaat tttgatcaaa gcactgctta aacaacttac
      301 agaaataggc atctgagtaa ttaaactgtc tggccgcatc ctgcatggag atctcgttca
      361 tataattttc ccggatatag gtgcgtatct tctcagctac ctggttcacc cgttctgttt
      421 ccgggtcttt cggcggctct gcatccgggt tccagggcca ttgctctttc cttttttccg
      481 cttccttttc tgcctctgtc ttttcttccc cgctgccgga agaatttttc ttattttcaa
      541 ctgccttatc cgtcaaccgc atggcctctt ccataacagc tgtcagttct tcttcatcac
      601 agggctttaa aagatagtcc agggcccgta ttactatggc acgttttgca taagaaaatt
      661 catcataggc agttaaaaac acgatgatac agtcttcatc catgctgcgg atcttctctg
      721 ccgcttctac accatttaat tccggcatgg aaatatccat gataatgatc tggctgtgct
      781 ctttttcata cagctgcact gcctcttttc cgttggtagc ttcactgatc tgtaaaatat
      841 ctccaaagcg cctcatcaaa cgtctggcaa gagccatacg ctcgatctgt tcatcatctg
      901 ctaaaagcac acgatacata aactttttcc tcccgtcttt ctctctgatc cccaatacat
      961 gtgccctgca ctttcgcagt tcacacgata tcttttggtg taaaagccag ttgtacggct
     1021 gttccgcacc cttttttact gtaaataaac atttccccat cttcatacag accatggacc
     1081 cggcggtaaa tatttcccac accaatgccg atctcatttt ctgtaccgtc atttaacgca
     1141 gcacggatct gttgtaaacg ttcttcttcc ataccaacac ccgtatctga cacagagatc
     1201 cacaaacgtt ctcctctcat ccaggtcctt ataaagatcc tgcctccctt gctctccttg
     1261 gaaaggccgt gtttaatgga gttttccacc aaaggctgca aaacaaagga gggaactaac
     1321 acatctaacg tttccggtgc acagtccgta tcataccgta ctctctggcc aaagcgcatt
     1381 ttctgcagat acatatagtc ctggatgatc ttcagttccc gctctaaagg cattaccgta
     1441 cctgctgatt ttaagttata ccggaacaga cggcttaaag cagtgatcat cttttccgta
     1501 gccactgcat cttcgatctg tgccgtactg gcgatcatat ttaaggtatt aaataagaaa
     1561 tggggattga tctggctttt taacatctga agacgcatgt catttaactt ctgctccatg
     1621 gcatgttttt cttccatgtt tcggatattt tccctggtag acgctttcat acggcagaat
     1681 tcctgtacca gacggctgat ctcatcttct ccctctgcca ctacatccgg ccctgtaaaa
     1741 tcattatccc caatgcgcac tgaatcagct gccagttcca caatcggctt tacaatatag
     1801 cggctggccg tccggttcat ctgccgcacc atccagaaag ccgccatccc gcagatcaga
     1861 ctgcataccg gtattaccac cagcatatac cgctgttttt catactggct gcttccgttt
     1921 cttacagtca gctgttctaa ctgccccgcc tgtcctgcca gatatccctg gatccggcgc
     1981 agcctgtaca cctcctgcat atccgcttcc ccaatggttt ccttttccat cagttccctg
     2041 cgaaatctgt cataattttc ataaaggtta tgcagggtcc aagttcttgc ataccgttct
     2101 tttccgatct ctttatattt ataaggtaat tcttccagca tcagttctgt atgtttagca
     2161 cttttttccc attcctgtct ggtttctttt gtctgctcct ggataaaccg gtcaaacgct
     2221 gcgctttctt cgtccatagc tgtccagaag gcaagacttc tggaattgct ctttaaaata
     2281 tttccaaatc ctcccagtcc gaaaaaagcc accagcatat tggcggcaat ggaaaggaat
     2341 acgactgcac caactgaaaa ggtaagatat ttcagttttt cctgcagcgg cagacagaac
     2401 cattttttca ttccttttct ccttctttca cacattcctt ttcttatttt atattaattc
     2461 tctcaatttt taaagatagg attgtgtaaa aaaatacccc ggcatatgat cctctctgcc
     2521 ggagtaacaa taaatcatat ctgactatag tggctggtcc ttgtccgatg gtacataagt
     2581 agcaatgtct tcgatctgac atccaagtat cctgcataga tcattcacag ttttcataga
     2641 aatatcctga ttatgcttta atcgatacaa cgtactgctt gacatatgat acttctttgt
     2701 cagtgtatac cagttttctt ctgatatttc cagtgttttc caaaacggtg aatagtcgat
     2761 catcttatgc tttctcctgt aaccccttga taggtcaagc ataagttaga ttttacatct
     2821 tgaatatctt cgtaataacg aatatactat aggtattaca gtgcctaaaa agggcatatc
     2881 gggaggtgtt atatgagctg gtacacagag gaagaaattg aaaaattact ggaagacgaa
     2941 gaactgagga aacgcattgc acgctttgtt accatgagcg gggaagaatt tttcgatgaa
     3001 gtgtacaccc atctctcacc ggaagaactg gaagaatatc tggaggaaaa ccccagtgaa
     3061 cgaaaatatc taaaacgtta tgaaccataa aaaacgataa aagcccaaag aaggaaggtc
     3121 tgtcaaatga caaaccttcc ttcttccggt tcttcttcaa aataatcttt ataatccacc
     3181 tcaatggcat ggcgcatacg cttcatgctg aaataaaggt gatctttcag tgctttttcc
     3241 agaccttttt cgtccttttc gcagatcagg cggaaaattt cttcatgctc tcctgtgatc
     3301 cgggtaaaat cggtctctgt tgtaaaatcc aacatcctga aacgggtata atgaagctgc
     3361 tgtgcctgaa gcatatccca cagtttctgc ctgcggaccg ctccaaacca gatagagtga
     3421 agctctgcgt ccatatggta aaactgctcc ggttcaaatc ccggtgtctg gatcagggct
     3481 ttctgcatag caatggcgtg acggatttct tccagggtca taggattttg gactttcaga
     3541 aaatccctca gcaccatggt ctccactgcc acacgcatgt agatcatctg tttgatgttg
     3601 tccagactta acagagtcac ataagtacct ctgtagggaa ctgtctctac aaatccctgt
     3661 tcctggagca gccgcaaagc atcccgcacc ggggtcctgg agaccccgaa gcgctcacag
     3721 atctcatttt cactgatcat acgccccggc tccagtttca gatctaaaat ttctcttttt
     3781 aatgtctcaa atacctgctc tccgcggttt ttgaaattca tctcgttcat tcagccaata
     3841 tcccacctat cttatttagg ctgcttgcca atgtaagcca ggatgccacc gtctacatac
     3901 agtaccagac catttacaaa gttggaagcg tcagatgcca ggaatactgc tggaccgcct
     3961 aagtcttctg catttcccca acgtcctgca ggagtcttgc ttacgataaa ctggtcaaat
     4021 ggatgtctgg aaccgtctgg ctggatctca cgaagaggtg cagtctgagg agttgcaatg
     4081 tatccaggtc cgataccatt acactggatg ttatactcgc cgtactcaga tgcaatgttc
     4141 ttggtcagca tctttaagcc gccctttgca gctgcataag cagatacagt ctcacgtcct
     4201 aattcactca tcatagagca gatgttgatg atctttccgt ggcccttctt gatcatgctc
     4261 gggataacag ccttggatac gataaatgga gcatttaagt ctacatcgat aacctgtctg
     4321 aactcagctg cgctcatctc acacattggg atacgcttga tgatacctgc attgtttacc
     4381 aggatatcga tcacgcctac ttctttttcg atctgtgcaa ctaatgcatt tacagcttct
     4441 tcattggtaa catcgcatac atagccatga gccttgacgc cttctgcctc ataagcagcc
     4501 agtcccttat ctaccagttc ctgcttgata tcgttaaata cgatggtagc gccagctcct
     4561 gccattgctt ttgcgatggc aaagccaatt ccgtaagatg cacctgtgat caatgctact
     4621 ttgccttcca gtgaaaagtt gtttaagtaa tctgccattt tattttcctc ctgtttttta
     4681 taattacatc atttcatttg gcttcatggt gtccatatca tcaaattcca tgttttcgcc
     4741 gcccattgcc cagataaatg tataattgga agtgccagct ccgctgtgga tgctccagga
     4801 tggactgatc actgcctctt catttttcat aacaatgtgt ctggtctcta ttggctctcc
     4861 catcatatgg aatacgacat tgtcaccagg tacttcaaaa tacatataaa cttccatacg
     4921 gcgctcatgt gtatgtgccg gcatggtgtt ccatacactt cccggctcta aaacagtcat
     4981 acccatagaa agctgacagg tctttaatac atccggatgg ataaactgat tgatcacacg
     5041 tttattggaa agagctgctt caccacatgg tctctttgct gccttttcga tgggaataaa
     5101 agtagttgta taagaagtat gagccggagc acttaccata taaaattttg ccggcttgga
     5161 agcatcttca ctttcaaagt aaacttcttt tgttcccttg gtaatgtaca ga
//
LOCUS       DBMDUA010000188         5204 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6120_length_5204_cov_1.992357, whole genome shotgun sequence.
ACCESSION   DBMDUA010000188 DBMDUA010000000
VERSION     DBMDUA010000188.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6120_length_5204_cov_1.992357"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            46..222
                     /gene="rpsU"
                     /locus_tag="ACZPCL_11500"
     CDS             46..222
                     /gene="rpsU"
                     /locus_tag="ACZPCL_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR1163126.1"
                     /translation="MSNVIVKDNESLDSALRRFKRNCAKAGIQQEIRKREHYEKPSVR
                     RKKKSEAARKRKYN"
     gene            complement(352..2097)
                     /locus_tag="ACZPCL_11505"
     CDS             complement(352..2097)
                     /locus_tag="ACZPCL_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990112 - RQC complex [Evidence IEA];
                     GO_function: GO:0001968 - fibronectin binding [Evidence
                     IEA];
                     GO_process: GO:0072344 - rescue of stalled ribosome
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein"
                     /protein_id="HIR1163127.1"
                     /translation="MAFDGITIANLRWEFAHTIEGGKIAKIAQPEKDELLITIKNNRE
                     NYRLQISASASLPLIYLTANNKTSPLTAPNFCMLLRKHIGSARIVSVKQPGLERILEF
                     ELEHLDELGDLCKKRLIVEIMGKHSNIIFCKEDGTIIDSIKHVSASMSSVREVLPGRE
                     YFIPQTIAKENPLTVTETVFRSCILSSPTSVQKALYGHLTGISPIVAEELCHLASIDS
                     DRSAGELTDPELTHLYHTFQLMMEDVKEGRFSPAVVYDGDTPVEYASVPLTCYEGKGY
                     RRETFESISTLLENYYASRDTITRIRQKSSDLRRIVQTALERSCKKYDLQLKQLKDTE
                     KREKYRIYGELLNTYGYELKGGEKSFKCINYYDNEEITIPLDPQLTARENAQKHFDKY
                     NKLKRTYEALSQLTMETKAEVDHLESVSSALDIALEENDLVQIKEELMEFGYVKKRRA
                     NEKRPKITSKPFHYISSDGYDIFVGKNNYQNEELTFKVASGNDWWFHAKGIPGSHAIV
                     KSNGQADLPDRVFEEAGALAAYYSKGRENDKVEIDYIQKKNIKKVAGAAPGFVIYHTN
                     YSLVASPRCDLKEVK"
     gene            2295..3992
                     /locus_tag="ACZPCL_11510"
     CDS             2295..3992
                     /locus_tag="ACZPCL_11510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1163128.1"
                     /translation="MNNFIQHVTGQLVWLGEHRGLFLLLIVELFAGVCLLIGFFQLKI
                     KKQNEAAGVFKGGSGSFGVPGNGDPVMILRCKDLYPVYITESFEQQLDVTGDDIKTDI
                     GIFLDKVGSGKDWKLWKKYRSWDGKQPFTSDFYLERMKSWYRLEITRSKDGLYDTFQF
                     RDITEDKKELEAAKEQLKIAESVSQSKTEFLSSMSHEIRTPMNGIIGMLTLAHGQLRG
                     HSAENYIIKAEQLSKYLLSVINDILDMSRIEAGKIELESKPFELAALAEKLRNMFQKN
                     VEAKGVAFYVEMKDVDVKYIVGDELRISQILVNFLSNAQKFTEKGEIRVTFRQLQKEN
                     GKVSLMFRVHDTGKGMDAKFISRIFKPFEQESQDITKQYGGSGLGMSITDRLVHLMGG
                     EIVIDSMLGKGSDFSVYLTLPIAEVSEIETEQEDETGTDFTFNGCHILMAEDNEINAE
                     IAVSILENEGAKVDVAVNGKDAVEKYAASAPGTYNFILMDIQMPVMNGRDAAKQIRSM
                     DRKDAGEIPIFALSADAFVEDQRLSAMSGMNGHFTKPIDFEEMRVQIGKILKGRRKY"
     gene            4010..>5204
                     /locus_tag="ACZPCL_11515"
     CDS             4010..>5204
                     /locus_tag="ACZPCL_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163129.1"
                     /translation="MGSLIKKAGVKGMAAGAVSLVLVAGGFHVLWEDYQRGNLFKPEL
                     FVKNRELQGNQIMFPEKENIQQNGNDPGENDNKKLEHDPEAKDPYGQEKKNEAAYELA
                     NNQMEADPESARNIFSDPSFDYAGGTGGTNWAPEGQSVVLAPDAQEKVNLPAGHSGLT
                     TEDTGNKDTPASSNGSGSDSGDTTISGGGSTGGGGGSGSNGNQDNTITPGGNDVPAPT
                     PTPDPTPTPTPTPDQPDNGDDTPTVDPDYPDDSKQPTLPSDPSLPGGEEITLPDFPSE
                     GLPEGDEAENAVLSIISSSDSVGELYRGAVLTDWKLLCSVYAYVDTPTGTYRLREYND
                     NFKIGDHPKIAEGDFTVTFYFRPNANSPWTEIEHEFSVKYCKMVVMGPEDEAGNRRTL
                     DSVYLG"
BASE COUNT         1515 a         1050 c         1240 g         1399 t
ORIGIN      
        1 gtattatgca caattacaca actggaggga ggttaggtgt gagcgatgtc aaacgtaatt
       61 gttaaagata acgagagctt agacagtgct ttacgcagat ttaaaagaaa ctgcgcaaag
      121 gctggcattc agcaggaaat tcgtaaaaga gagcattacg agaagccaag cgtaagacgt
      181 aagaaaaagt ctgaagctgc tcgtaagcgt aagtacaact aattctcgca cggaattggt
      241 tcacttgaag taaaataaat cggccggtcg tcaggtttgc ctgatggacc ggctgtttta
      301 ttttgtataa agttttgatt tatgccccgc catctgacgg ggcatgctta gctattttac
      361 ttcttttaag tcacatctcg gtgaagctac cagggagtag ttagtatgat agattacaaa
      421 tcccggtgca gctcctgcca ctttcttaat gttcttcttc tggatgtagt cgatctcaac
      481 cttatcattt tcacggcctt tggaataata agctgctaaa gcaccagctt cttcaaagac
      541 ccggtctggc agatctgcct gcccgttgct ctttacgatc gcatgggagc ctggaatacc
      601 ttttgcgtgg aaccaccagt cgttgccgct tgccactttg aaagtaagct cttcgttctg
      661 atagttgttc tttcctacga aaatatcata tccgtcagaa gaaatataat ggaaaggctt
      721 gcttgtgatc tttggccgct tttcgttggc acggcgtttc ttcacataac caaattccat
      781 taattcttcc ttgatctgca ccagatcatt ttcctctaag gcaatatcaa gggcagaact
      841 tacagactcc agatggtcaa cttcggcttt ggtttccatg gtcagctggc ttaaggcttc
      901 ataagtacgt tttaatttat tatacttgtc aaaatgcttc tgggcattct cacgggctgt
      961 cagctgcggg tctaatggga tcgtgatctc ttcattatca taataattaa tacatttaaa
     1021 agatttttcc ccacccttta actcgtaacc ataggtgttt aacagttctc cgtagatcct
     1081 gtatttttca cgcttttcgg tatcttttaa ctgctttaac tgcaggtcgt attttttgca
     1141 gctgcgctct aaggcagtct gaacaatacg gcgcaggtca gaagatttct gacggatacg
     1201 ggtaatggta tctctggagg cgtaataatt ttccaggaga gtacttatag actcaaacgt
     1261 ttccctgcgg tatccctttc cttcatagca ggtcagggga acagaagcat attctacagg
     1321 tgtatccccg tcatatacaa cggcagggct gaaacgtcct tctttgacat cctccatcat
     1381 cagctggaat gtatgataca gatgagtcag ttccgggtct gtcagttcgc cggcgcttcg
     1441 gtcggagtcg atggaagcca gatggcaaag ttcctccgca acaatagggc tgatgccggt
     1501 aaggtgaccg tataatgctt tctgcacaga agtaggagag gataaaatac agctgcggaa
     1561 cacagtttct gtaacggtca gaggattttc ctttgcaatg gtctgcggaa taaaatattc
     1621 ccgtcccgga agcacttcac gcacagaact catgctggca gatacatgct tgatactgtc
     1681 aatgatggtt ccatcttcct tacagaaaat aatattactg tgttttccca tgatctctac
     1741 gatcaggcgc tttttgcaca gatcccccag ctcatctaaa tgttccagct caaactctaa
     1801 aatacgttcc agacccggct gctttacaga aacaatgcgg gcgctgccaa tatgctttct
     1861 taaaagcata cagaaattag gcgcagtgag agggctggtc ttattatttg cagtaagata
     1921 aataagagga agacttgcgc ttgcagagat ctgcagccgg tagttttctc tgttgttttt
     1981 tatagtaata agcaactcat ccttttccgg ctgggcgatc tttgcgatct tcccgccttc
     2041 tatggtatgg gcaaattccc atcttaagtt tgctatggta ataccgtcaa atgccatgta
     2101 tattcactcc tgtcgatcaa aaataggcaa tactgtatga aacagactgc tcttataagg
     2161 ataccacatt ttctttcatt agaaaagtat tacaacaata tggaaaaact gctgtacact
     2221 ggaaaaaaat aacctataat aaaatgtaaa agttctgtaa atgctttcat taatgcgtat
     2281 tggaggaatt tcctatgaat aattttatac agcatgtgac agggcagctg gtgtggttgg
     2341 gagaacacag aggcctgttt ttgctgttga tcgtagaatt gtttgccggt gtgtgtttac
     2401 tcatcggttt ttttcagtta aagatcaaaa aacagaatga agccgcaggg gtgtttaaag
     2461 gcggttctgg atcctttggt gtaccgggaa acggagatcc ggtaatgata cttcgctgta
     2521 aagatctgta tcctgtatac ataacagaaa gctttgaaca gcagcttgac gttaccggtg
     2581 atgacataaa aacagatata ggtatatttt tggataaagt aggttcggga aaagactgga
     2641 agctgtggaa aaagtatcgt tcctgggatg gaaaacagcc ctttacttcg gatttttatc
     2701 tggagagaat gaaaagctgg tacagactgg agattacccg cagtaaggac ggtttatatg
     2761 atacattcca gttccgtgat ataacagaag ataagaaaga actggaagct gcaaaagaac
     2821 agttaaagat cgcggaaagc gtaagccagt caaagacaga attcctttcc agtatgtccc
     2881 atgagatccg tacgcctatg aatggtatta ttggtatgct gacactggca catggtcagt
     2941 taagaggcca tagtgctgaa aattatatta taaaggcaga gcagctttcc aagtatctgc
     3001 tttctgttat caatgatatt ctggatatgt cccgtattga ggcaggaaag atcgagctgg
     3061 agagcaagcc atttgaactt gcggcgctgg cagaaaagct gcgtaacatg ttccagaaaa
     3121 acgtagaagc caaaggcgtg gcattctatg tggaaatgaa agatgtagat gtaaaataca
     3181 tagttggcga tgaattgcgt atttcccaga tcctggtaaa ctttttatcc aatgcccaga
     3241 agtttacaga aaaaggagag atccgtgtta cattccgcca gcttcagaaa gaaaatggca
     3301 aagtaagcct gatgttccgt gttcatgata caggaaaagg tatggatgct aaatttatca
     3361 gccgtatttt caagcctttt gaacaggaaa gccaggatat caccaaacag tacggtggat
     3421 ccggtcttgg tatgtctatt acagaccgtc tggtccatct gatgggcggt gagatcgtca
     3481 ttgacagtat gcttggcaaa ggctctgatt tcagcgtata tctgacactt ccgatcgcag
     3541 aagtttctga gatagaaaca gaacaggaag acgagacagg aacagacttc acctttaacg
     3601 gatgccacat ccttatggca gaagataatg agatcaacgc ggagatcgca gtcagcattc
     3661 tggaaaatga gggtgctaaa gtcgatgtag ccgtaaacgg taaagatgct gtggaaaaat
     3721 atgcagcttc tgcaccggga acttataact ttatccttat ggatatccag atgccggtga
     3781 tgaacggccg tgatgcggca aaacagatcc ggagtatgga tcgaaaagat gctggagaga
     3841 tacctatctt tgctctttct gcagatgcct ttgtagaaga tcagagactt tctgctatga
     3901 gcggtatgaa tggtcacttt acgaaaccga ttgattttga agagatgcgt gtgcagatcg
     3961 gcaagatatt aaaaggacgg agaaaatatt gaaaaagcaa aagaaaaata tgggcagtct
     4021 tataaaaaaa gcaggtgtaa aagggatggc agcaggtgct gtatccctgg tccttgttgc
     4081 aggcggcttt catgttttat gggaagatta tcagaggggt aacctgttta agccggaact
     4141 gtttgtaaaa aacagggaat tgcagggtaa ccagatcatg ttcccggaga aagaaaatat
     4201 acagcaaaat ggaaatgacc cgggagaaaa tgacaataaa aaactggagc atgacccgga
     4261 ggcaaaggat ccctatggtc aggaaaagaa aaacgaagct gcctatgaac tggccaataa
     4321 ccagatggaa gcagatccgg aaagtgccag aaacattttc tcagatcctt cctttgatta
     4381 tgcaggaggc acaggcggta caaactgggc tcccgaagga cagagtgtag tcctggcgcc
     4441 ggatgcccag gaaaaggtca accttccggc aggacattct ggtctgacta cagaagatac
     4501 aggaaataag gatacccctg cttcttccaa tggttcaggc agcgacagcg gtgatactac
     4561 gatcagcggc ggcggttcaa ctggcggcgg tggtggctcc ggttccaatg gaaatcagga
     4621 taataccata acacccggtg gaaatgacgt acctgcgcca actccaacgc cggatccgac
     4681 tcctacacca accccaacac cggatcagcc ggataatgga gatgatacgc cgactgtaga
     4741 cccggattac ccggatgact ccaagcagcc taccctgcca agcgaccctt ctttacctgg
     4801 tggagaagaa attacgcttc cggattttcc aagtgaagga ctgcctgaag gggatgaggc
     4861 tgaaaatgca gtactttcta ttataagttc ctcagattct gtaggtgaac tttaccgggg
     4921 cgctgtactt acagactgga aactgctgtg ttctgtttat gcatatgtag acacacctac
     4981 gggaacttat cgtctgagag agtataacga taattttaag attggagatc atcccaagat
     5041 agcagagggg gattttactg ttacctttta ttttagaccc aatgccaaca gcccgtggac
     5101 agagatcgaa catgaatttt ctgtaaaata ttgcaagatg gttgtaatgg gaccggaaga
     5161 tgaggctgga aacagacgta ccttagacag cgtatatctg ggag
//
LOCUS       DBMDUA010000189         5191 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6137_length_5191_cov_2.594892, whole genome shotgun sequence.
ACCESSION   DBMDUA010000189 DBMDUA010000000
VERSION     DBMDUA010000189.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6137_length_5191_cov_2.594892"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            3..605
                     /gene="plsY"
                     /locus_tag="ACZPCL_11520"
     CDS             3..605
                     /gene="plsY"
                     /locus_tag="ACZPCL_11520"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR1163130.1"
                     /translation="MFGIFQTGYIYGRVHGIDIRKYGSGNSGSTNALRVMGKKAGLTV
                     FLGDFLKTVIPCMAVRFVFKDQPDYAYVYMLYIGLGVILGHNYPFYLHFKGGKGIAAT
                     AGIIFSIDWRLTVLCLAVFVLIVAVTRYVSLGSLVVSVILLVWNIYMGRCGAYSLSAI
                     SSIEYSAVTAVIAAMAFWRHRANIVRLIHGNENKIGAKKS"
     gene            630..1643
                     /locus_tag="ACZPCL_11525"
     CDS             630..1643
                     /locus_tag="ACZPCL_11525"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR1163131.1"
                     /translation="MASVGVIGSGTWGTSLAILLNGNGHKVQLWSAVPAEVESLSVNR
                     KHPNLGDTPIPEAIEITGDLKAAMKDKDLLVLSVPSVYVRQTAAKMKPWLKPGQIVTN
                     VAKGIEEATLKTLSEVIEEELPEAEVTVLSGPSHAEEVSRGLPTTCVAGAHSKAAAEY
                     VQSLFMSPVFRVYTSPDMLGIELGGALKNVIALAAGTADGLGCGDNTKAALITRGIAE
                     ITRLGTVMGGKPETFSGLTGIGDLIVTCASMHSRNRRAGILIGKGYTMDEAMKEVKMV
                     VEGVYSAKAALKLAEKYHVTMPIVEQVNEVLFDGKSAKDALAELMLRDKRMENTCLVW
                     DKE"
     gene            1805..3034
                     /locus_tag="ACZPCL_11530"
     CDS             1805..3034
                     /locus_tag="ACZPCL_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1163132.1"
                     /translation="MKKRFLSAGLAVVMAASLTACGGSDSASTTAAAAGESKAEGESA
                     AAGESSAAADGAVFKIGGIGPVTGGAAVYGLAVQHGAEVAVKEINEAGGINGAQIEFQ
                     FQDDEHDAEKSVNAYNTLKDWGMQALVGTTTSAPCIAVADKTAADNMFQITPSGSAVE
                     CAANPNVFRVCYSDPAQGTASAKYIGEHKLASKVAVIYDSSDVYSSGIYEKFAQEAPN
                     QGLEVVDAEAFTADSNKDFSTQLQKAKESGAELVFLPIYYTEASLILQQANTMGYAPQ
                     FFGCDGMDGILQVENFDVKLAEGLMLLTPFAADATDDLTVNFVKSYEEAYGETPIQFA
                     ADAYDAVYIIKAAMEEANVTPDMSASDICDAMEGVMTQIKVDGLTGSNMSWTEDGEPE
                     KDPKAVQVVDGAYKAME"
     gene            3152..4033
                     /locus_tag="ACZPCL_11535"
     CDS             3152..4033
                     /locus_tag="ACZPCL_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1163133.1"
                     /translation="MSFINYLINGVSLGSVYAIIALGYTMVYGIAKMLNFAHGDIIMI
                     GSYVVFVTVSSMGLPPMLGVLLAVAACTLLGVVIERVAYKPLRSASPLAVLITAIGVS
                     YLLQNVALLIFGADTKSFTSVVKIPALKLADGQLNITGETIATILSCIVIMVCLMAFI
                     NRTRAGQAMLAVSEDKGAATLMGINVNGTIALTFAIGSALAAIAGVLLCSAYPSLTPY
                     TGSMPGIKAFVAAVFGGIGSIPGAFIGGVLLGVIEILSKAYISSQLSDAIVFSVLIIV
                     LLVKPTGILGKKISEKV"
     gene            4042..5112
                     /locus_tag="ACZPCL_11540"
     CDS             4042..5112
                     /locus_tag="ACZPCL_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1163134.1"
                     /translation="MKSKAKQKTFINNMITYAIVVVAYIVVQILMGTGHMSSLMKGLL
                     VPFCIYVIMAVSLNLTVGILGELSLGHAGFMCVGAFAGALFSKCMKGSMNPTVSLIIA
                     VIIGAVVAAVFGILIGIPVLRLKGDYLAIVTLAFGEIIKNIINAVYLGRDGSGFHISL
                     KDSMSLNMDADGEVLIKGAQGITGTPQAATFTIGVILVLITLFIVMNLVNSRTGRAIM
                     AIRDNRIAAESIGINITKYKLMAFSISAGLAGVAGVLYAHNLTTLTALPKNFGYNMSI
                     MILVYVVLGGIGSIRGSVIATVILYLLPEMLRGLSNYRMLMYAIVLILAMLFNSAPQF
                     VVIRERMFAALKGNKKPAKEAK"
BASE COUNT         1424 a         1050 c         1310 g         1407 t
ORIGIN      
        1 atctgtttgg tatcttccag actggttata tttatggaag ggtccacggg atcgacatcc
       61 gcaaatacgg aagcggaaat tccggcagta ctaatgcgct gcgtgtaatg ggaaagaaag
      121 caggccttac tgtatttttg ggagatttct taaaaacagt gatcccctgt atggcagtgc
      181 gctttgtttt taaagaccag ccggactatg catatgtgta tatgctttat attggtttag
      241 gagttatctt aggacataat tatccttttt atctgcattt taaaggcgga aaaggaattg
      301 cggcaacagc cggtatcatt ttctcaattg actggagact gactgtttta tgtctggcgg
      361 tatttgtact gatcgtggct gtaacacgtt atgtttcttt aggttccctg gtggtttctg
      421 tgatccttct ggtatggaat atctacatgg gaagatgcgg agcttacagt ttaagcgcta
      481 tttcttctat agaatatagc gcagtgacag cagtgatcgc tgctatggca ttctggcggc
      541 acagggcaaa tatcgtccgt ctgatccatg gaaatgaaaa taagatcggc gcaaaaaaat
      601 cataaaaaat ggcaaaggat ggtggggtta tggcatcagt tggagtaatc ggatcaggaa
      661 catggggaac atcactggcg atccttttaa acggaaatgg tcataaagtg cagctctggt
      721 cagcagttcc cgcagaagtg gaaagtttat cagtaaacag aaagcatccc aatttaggag
      781 atacgccaat accggaagca attgagatca ccggtgactt aaaggcggct atgaaggaca
      841 aagatctgtt ggtcctgtcc gtgccatctg tctacgtgag acagacagca gcaaaaatga
      901 aaccatggtt aaaaccaggt cagatcgtta caaatgtagc aaagggaatt gaagaggcaa
      961 ctttaaaaac tctttcagag gtcattgaag aagaacttcc agaagcagag gtaaccgtcc
     1021 tttccggacc gagccatgca gaagaagtga gcagaggtct tcctaccacc tgtgtagcag
     1081 gcgctcacag caaggcagca gcagaatatg tgcagagttt atttatgagt cctgtattcc
     1141 gcgtatacac gagtccggat atgttaggca ttgagctggg cggagcatta aagaatgtca
     1201 ttgccctggc ggcaggcacg gcagatggtt taggatgtgg ggataacaca aaggcagccc
     1261 tgatcaccag aggtattgca gagatcaccc gtttaggaac ggttatgggc ggaaagccgg
     1321 agaccttctc aggactgact ggtatcggcg atctgatcgt tacctgtgcc agcatgcaca
     1381 gccgtaatcg cagggcaggt atcctcatcg gcaaaggcta taccatggat gaggcaatga
     1441 aagaagtaaa gatggtagtg gaaggcgttt attctgcaaa ggcagcttta aagctggcag
     1501 aaaaatacca tgtgaccatg cccatcgtag aacaggtgaa tgaagtttta tttgatggca
     1561 agtctgcaaa agatgccctg gcagagctga tgctgcggga taagaggatg gaaaatacct
     1621 gtcttgtctg ggataaggaa taactcccat aactacaggt tatgaaacaa cctatccttc
     1681 tcatattttt ttataaaaag gtattgacaa ggcaatagtc agtttttata atgtaaaaag
     1741 tttaagaaca aatgtatctg tatgaaagac atatcagata cagtcatgtg aggaggaaac
     1801 tattatgaaa aaaagattct taagcgctgg tttagcagta gttatggcag catctttaac
     1861 cgcatgcggc ggctctgact ccgcatctac cacagcagca gctgctggtg aaagcaaggc
     1921 agagggagaa agcgcagcag caggcgaaag cagtgcagca gcagatggcg ctgtatttaa
     1981 aatcggtggc atcggccctg taaccggtgg tgcagcagtt tatggtctgg ctgtacagca
     2041 tggtgcagaa gttgctgtta aggagatcaa cgaggctggc ggtatcaatg gcgcacagat
     2101 cgaattccag ttccaggatg atgagcatga cgcagagaag tctgttaatg cttacaatac
     2161 tttaaaagac tggggcatgc aggctctggt aggaactact acctccgctc catgtatcgc
     2221 agttgctgat aagaccgcag ctgataatat gttccagatc actccatccg gttccgcagt
     2281 agagtgtgcc gcaaatccaa acgtattccg tgtatgctat tctgacccgg ctcagggtac
     2341 tgcatctgct aagtacattg gtgagcacaa actggcaagc aaggttgctg taatctatga
     2401 cagctctgat gtatactcta gcggtatcta tgagaagttt gctcaggaag ctccaaacca
     2461 gggccttgaa gttgttgacg cagaagcatt taccgcagac agcaacaagg atttctctac
     2521 tcagttacag aaagcaaaag agtccggcgc agagctggta ttcttaccaa tctactatac
     2581 agaggcttct ctgatcttac agcaggctaa taccatgggc tatgctccac agttcttcgg
     2641 atgcgatggt atggatggta tcttacaggt tgagaacttt gatgtaaagc tggcagaagg
     2701 actgatgctg ttaactccat ttgctgcaga tgctaccgat gatctgacag taaactttgt
     2761 aaagagctac gaagaagcat atggcgagac tccaatccag ttcgctgcag atgcttatga
     2821 tgcagtttac atcatcaagg ctgctatgga agaagcaaat gtaactcctg atatgtccgc
     2881 aagtgatatc tgcgacgcta tggaaggcgt tatgacccag atcaaggtag acggtctgac
     2941 cggttccaac atgagctgga ctgaagacgg cgagcctgag aaggatccta aggctgttca
     3001 ggttgtagac ggcgcttaca aggcaatgga ataatcaatt tcagaaggtt taaaatctga
     3061 tcaataaccc aaaggagggt tgtcgttaag acaaccctct tggttttata ggggaattta
     3121 aactttcaat atttaatgaa gaggtgtatg tatgagcttt atcaactatt tgatcaatgg
     3181 cgtcagcctg ggcagtgtat atgcgatcat cgcactggga tataccatgg tttatggtat
     3241 tgcgaagatg ctgaatttcg ctcacggtga tattattatg ataggaagct atgttgtgtt
     3301 cgtcacagtc agctccatgg ggcttccgcc tatgttaggc gtcctgctgg cagtagcagc
     3361 atgcacattg ttaggtgttg tcattgagcg ggtggcatat aagccattac gtagtgcgtc
     3421 acctcttgct gtactgatca cagccatcgg tgtcagctac ctgctccaga acgtagcact
     3481 gctgatcttt ggcgcggata caaaatcatt tacttctgtt gtaaagatcc cggcattaaa
     3541 gctggcagac ggccagttaa atattaccgg cgagaccatt gcaaccattc tttcctgtat
     3601 cgtgatcatg gtatgcctga tggcatttat taaccgtaca agagcaggac aggctatgtt
     3661 agctgtttca gaagataagg gtgcggcaac cttaatgggt atcaatgtaa acggaaccat
     3721 tgctttaact ttcgctatcg gttctgcact ggcagccatt gcaggtgtgc tgttatgctc
     3781 cgcttatccg tccttaaccc catatacagg ttccatgcca ggtatcaagg cattcgtggc
     3841 agcagtattt ggcggtatcg gatccatccc aggcgctttt atcggaggcg ttctcttagg
     3901 tgtgatcgag atcctttcca aggcgtatat ttcttcccag ctttcagatg ccatcgtgtt
     3961 ctcagtactg atcattgtac tgttagtaaa acccaccggt attcttggca agaagatcag
     4021 tgagaaagtg taggtgcggg tatgaaaagt aaagcaaagc agaagacatt tatcaacaat
     4081 atgatcacat atgccattgt ggttgtggca tatatcgttg ttcagatctt aatgggcacc
     4141 ggacatatgt ccagcctgat gaaaggactt ttagttcctt tctgtatcta tgttattatg
     4201 gcagtttcct taaacctgac agtaggtatc ctgggtgagt taagccttgg acacgcaggt
     4261 tttatgtgcg taggcgcttt cgccggagcg ttgttctcca agtgcatgaa gggctctatg
     4321 aatccgacgg tttctctgat cattgcagta atcatcggag cggtagtagc agctgttttc
     4381 ggtattctca tcggtatccc ggtactccga ttaaaaggtg actacctggc tatcgtaact
     4441 ctggcgtttg gtgagatcat taaaaatatc atcaatgctg tatatctggg aagagatggt
     4501 tccggcttcc atatttcttt aaaagactcc atgtccttaa atatggatgc agacggcgaa
     4561 gtgctgatca agggtgccca gggtattacc ggtacacctc aggcagcaac ttttaccatt
     4621 ggtgtgatcc tggtactgat cactttattt attgtaatga acctggtcaa ttcccgtaca
     4681 ggccgcgcta tcatggctat ccgcgataac cgtattgcag cagagtctat tggtatcaac
     4741 atcacaaaat ataaactgat ggcattcagt atttccgcag gtcttgctgg cgtggcaggt
     4801 gttctctatg cacacaacct gacaacactg accgctctgc caaagaactt tggttataac
     4861 atgtccatta tgatcctggt ttatgtagtt cttggcggta tcggaagcat ccgcggttct
     4921 gtgatcgcta ccgttatcct gtatctcctt ccagagatgc tccgaggact gagcaattac
     4981 cgtatgctga tgtacgccat cgtgctgatc ttagcgatgc tgtttaactc tgcacctcag
     5041 tttgtagtta ttcgtgagag aatgtttgca gcattaaagg gaaataagaa accggcaaag
     5101 gaggcaaaat aatatggcat tactggaagt aaagaattta aggaggcaag ggcaaaactt
     5161 gtcagcattg aagcagatgt gattaaaaat t
//
LOCUS       DBMDUA010000190         5169 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6154_length_5169_cov_2.179953, whole genome shotgun sequence.
ACCESSION   DBMDUA010000190 DBMDUA010000000
VERSION     DBMDUA010000190.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6154_length_5169_cov_2.179953"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="ACZPCL_11545"
     CDS             complement(<1..464)
                     /locus_tag="ACZPCL_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HIR1163135.1"
                     /translation="MSLEKLFHLKENHTDVKTEVLAGITTFMTMAYILAVNPGILGAT
                     GMDSGAVFTATAVSALFATLCMAFMANYPFVLAPGMGLNAYFAYTVVLQMGYSWEMAL
                     TAVFAEGIIFILLSLTNVREKIFNAIPMTLKHSVSAGIGLFIAFIGLQNAKIV"
     gene            complement(688..>1691)
                     /gene="pepT"
                     /locus_tag="ACZPCL_11550"
     CDS             complement(688..>1691)
                     /gene="pepT"
                     /locus_tag="ACZPCL_11550"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIR1163136.1"
                     /translation="DTSPALSGKDVKPQFVENYDGGDICLNKEKDIWLLTSDFPEMKN
                     YKGKTLITTDGTTLLGADDKAGVAEIMTMAAWFLSHPEEKHGRICIAFTPDEEVGRGA
                     DFFDVEGFGAEAAYTVDGGALGELEYENFNAASGHVTIQGKGIHPGSAKGKMKNALLI
                     AMEFQALLPAFENPMYTEGYEGFYHLDHMEGDVEGAQMAYIIRDHDREKFEQKKAFFE
                     KAAEFLNAKYGAGTVTAVVKDSYYNMKEKIEPHMELIHIAENAMRKNGVTPVISPVRG
                     GTDGARLSYMGLPCPNLCTGGHNYHGKYEYVCVESMEQTVNILTEIVKSFAVGKNVD"
     assembly_gap    1692..1701
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1702..1955)
                     /locus_tag="ACZPCL_11555"
     CDS             complement(<1702..1955)
                     /locus_tag="ACZPCL_11555"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574172.1"
                     /note="catalyzes the release of the N-terminal amino acid
                     from a tripeptide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIR1163137.1"
                     /translation="MKIIENSVTERFLRYISYDTQSKEEGEQVPSTTKQLELGKLLTT
                     ELKEMGVANVRMDEHGYIYGEIPANTEEKITSLGFIAHMDT"
     gene            complement(2091..3401)
                     /gene="hisIE"
                     /locus_tag="ACZPCL_11560"
     CDS             complement(2091..3401)
                     /gene="hisIE"
                     /locus_tag="ACZPCL_11560"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HIR1163138.1"
                     /translation="MDNKELIAGFGYQEGRISSWDGKRTYTQAAPQMAKHCCDNGADE
                     ILIYDWSVTDDDHEAAIAVIKEVCREADAPVIAGGRVKRMEDVKKYLYAGASAVFLDM
                     SKEENVDMIKEASDRFGKEKIYAYLPTDAYLDHVKDYKAAGASVMLLNTAGSVPSLLE
                     MASISDSEAPFLFFLQAKDDAKDTAESLKNAFGCGNICGAVLTFTEDAMDTSMTIKQS
                     LKAAGISVDTFESSVDWKDFKLNSDGLIPVIVQDYKTNEVLMLAYMNEEAFNNTLATG
                     RMTYFSRSRQAQWVKGETSGHFQYVKSLKIDCDNDTLLATVKQIGAACHTGNRSCFYT
                     TLAEKEYKETNPLKVFEDVFNVILDRKKHPKEGSYTNYLFDKGIDKILKKVGEEATEI
                     VIAAKNPDPEEVKYEISDFLYHMMVLMAEKGVTWEEITEELANR"
     gene            complement(3423..4142)
                     /gene="hisA"
                     /locus_tag="ACZPCL_11565"
     CDS             complement(3423..4142)
                     /gene="hisA"
                     /locus_tag="ACZPCL_11565"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR1163139.1"
                     /translation="MQLYPAIDMKNGKCVRLRQGAFKDITVYSEHPEEIAALWQEKGA
                     TFLHLVDLDGALAGRSVNEETIKKIVSSVNIPVEIGGGIRSEEAIKNMLALGVKRVII
                     GTKAVEEPEFLKDMVEKFGADAIAAGVDAKDGMVAIQGWEQVSTVTATDLCLKMKEYG
                     VKHIIYTDISRDGMLSGPNIPATKKLTEETGLDIIASGGVSCMEDLQALYDAGISGAI
                     MGKALYEKRVDLEEAVRRFEK"
     gene            complement(4191..4778)
                     /gene="hisB"
                     /locus_tag="ACZPCL_11570"
     CDS             complement(4191..4778)
                     /gene="hisB"
                     /locus_tag="ACZPCL_11570"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR1163140.1"
                     /translation="MERIAQITRNTNETKINMTLNLDGSGKANIHTGIGFFDHMLNSF
                     ARHGFFDLDLTVDGDLEVDTHHTIEDTGIVLGQAIRKAVGDKKGICRYGSQILPMDEA
                     LVICALDLCGRPYLVYDLNIDREKVGDLETEMVREFFYAVSYGGEMNLHLRQMSGSNN
                     HHIIEAAFKAFAKALDGATGYDPRLTDVLSTKGSL"
     gene            complement(4895..>5169)
                     /locus_tag="ACZPCL_11575"
     CDS             complement(4895..>5169)
                     /locus_tag="ACZPCL_11575"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR1163141.1"
                     /translation="MTKIRNAGAIFIGEYSSEPLGDYFAGPNHILPTNGTAKFFSPLG
                     VDDFIKKSSIIYYSREALEKAHKDIEDFAEAEHLTAHANSVRVRFE"
BASE COUNT         1277 a         1260 c          993 g         1629 t
ORIGIN      
        1 acgatctttg cgttctgtaa accgataaaa gcgatgaaaa gaccgatacc agcggataca
       61 gagtgcttta atgtcatagg aatggcatta aagatcttct cacgtacatt ggtcagtgac
      121 agaaggatga aaatgatacc ttctgcaaat acggctgtta aagccatttc ccatgagtat
      181 cccatctgta aaactacggt ataagcaaag taagcgttta agcccatacc tggtgcaagt
      241 acaaatgggt agtttgccat aaatgccata cataaagtag caaacaaagc tgacactgcg
      301 gtggctgtaa agactgcacc gctgtccata ccggtagcgc ctaagatacc cgggttaaca
      361 gccaggatat aggccatagt cataaaggtg gtgattcctg ctaacacctc tgttttgacg
      421 tctgtgtggt tctccttcag gtgaaacaat ttttccagac tcataaaaaa tcctccttct
      481 catgtattga tctctgataa ctaccagaga cctgttcttc ttcctggcat tttttagaag
      541 tcccctgcct cctttccccc gattgccaga aatccaaaca tttttataca tacattttag
      601 cactttttta atgttttgta tatacaaaat aaaattttaa attgccaaag agaaaatctt
      661 attgtataaa tctgattttt ttgtctatta atctacattt tttcctactg caaaactttt
      721 tacaatctct gtaagaatgt tgaccgtctg ttccatagac tccacacata catattcata
      781 ttttccgtga taattatgtc cacctgtgca aaggtttggg cacggaagtc ccatgtagga
      841 cagtctggcg ccgtctgttc caccgcgtac cggggaaata accggagtaa ctccattttt
      901 tctcatagca ttttctgcaa tgtggatcag ttccatatgt ggctctatct tctctttcat
      961 gttgtaatag gagtctttta caacagcagt tactgtaccg gccccgtatt ttgcatttaa
     1021 aaactctgct gctttttcaa aaaatgcttt cttctgttca aatttctccc tgtcatggtc
     1081 acggatgata tatgccatct gggcgccttc cacatcacct tccatatggt ctaagtgata
     1141 aaaaccttcg tagccttctg tatacatagg attttcaaat gctggaagca gtgcctggaa
     1201 ttccatagcg atcagcaggg catttttcat ctttcccttg gcgcttcccg gatggatgcc
     1261 ttttccctgg atggtcacat ggccggaagc tgcattaaag ttttcatatt ccagctcccc
     1321 taaagcacct ccatctactg tataagcggc ctctgcccca aagccttcta catcaaagaa
     1381 atctgctcct cttcccactt cctcatctgg tgtaaaggca atgcagatcc tgccatgttt
     1441 ctcttccgga tggcttaaaa accaggctgc catggtcatg atctctgcca cacctgcttt
     1501 atcatctgct cctaacaatg tagtgccgtc tgtagtgatc agtgtttttc ctttataatt
     1561 tttcatttcc gggaaatcgg aagtaagaag ccagatatct ttttctttgt ttaaacagat
     1621 atcaccgcca tcataatttt ccacaaactg aggctttacg tctttaccag aaagagccgg
     1681 tgatgtatcc annnnnnnnn ngtatccata tgggcaatga aacctaaaga agtaatcttt
     1741 tcctctgtat ttgccgggat ctcgccataa atatatccat gctcgtccat gcgcacattg
     1801 gcaacaccca tttcctttaa ctcagtagtc agaagttttc caagttctaa ctgctttgtt
     1861 gtacttggaa cctgttcgcc ctcttctttt gactgggtat cgtaggaaat atagcgtaaa
     1921 aaacgttctg taacagaatt ttctattatt ttcatttcat cgtcctccaa aatcatattt
     1981 attaagataa ttttaccacg gatccgctca ttatgcaaaa aggacagccg gtctttcgat
     2041 ccagctgtcc caaatgaaag caatctgctt tcgtacatct tttgtatatc ttatctgttt
     2101 gccagttcct ctgtgatctc ttcccaggta acaccttttt ctgccattaa taccatcata
     2161 tggtacagga aatcggagat ctcgtatttt acttcctccg gatccgggtt cttggctgca
     2221 ataacgatct cagtagcttc ttctcccact ttctttaaga tcttatcaat tcccttatca
     2281 aaaagatagt tggtataaga accttccttt ggatgttttt tgcggtctaa gatcacattg
     2341 aaaacgtcct caaatacttt taatggattg gtctctttgt attccttttc tgccagtgtg
     2401 gtgtagaagc aggaacggtt gcctgtgtgg caggctgcgc cgatctgctt tacagttgca
     2461 agaagtgtat cattgtcaca gtcgatcttt aaggatttta catactggaa atgtccgctg
     2521 gtttctccct ttacccactg cgcctgacgg ctgcggctga aataggtcat gcggccggta
     2581 gccagcgtat tgttaaatgc ctcttcattc atgtaagcta acattaatac ttcattggtc
     2641 ttataatcct gaacgatcac agggatcagc ccgtcgctgt ttaacttaaa gtccttccag
     2701 tctacggaag attcaaaggt atctacggaa attcccgctg cctttaaaga ctgtttaatg
     2761 gtcatggaag tatccatggc atcctctgta aaggtaagaa ccgcaccgca gatatttccg
     2821 cagccaaagg catttttcag gctttctgct gtgtccttgg catcatcctt tgcctgtaag
     2881 aagaataaaa atggtgcttc actgtcgctg atagaagcca tttccagtaa agagggaacg
     2941 ctgcctgctg tattaagaag catgacggat gcacctgctg ccttatagtc ctttacatga
     3001 tccagataag catctgttgg cagataagcg tagatcttct cttttccaaa gcggtcagat
     3061 gcctctttga tcatatccac gttttcttct ttgctcatgt ctaaaaatac agcgctggct
     3121 cctgcataaa gatatttctt tacatcttcc atgcgtttta ctctgccgcc tgcaatgacc
     3181 ggggcatccg cttccctgca gacctctttg atcactgcaa tggcagcctc atggtcatca
     3241 tctgttacag accagtcata gatcagaatt tcatccgctc cgttatcgca gcagtgtttt
     3301 gccatctgag gtgcagcctg ggtataggtt ctctttccgt cccagctgct gatacggcct
     3361 tcctggtagc caaaacctgc gatcagttct ttattatcca tgtccattta ctccttgtcc
     3421 gttcattttt caaaacggcg gactgcctct tccaagtcca cacgtttttc ataaagagct
     3481 tttcccatga tcgctcctga gatccctgca tcataaagag cctgcaggtc ttccatacag
     3541 gatacgccgc cggatgcgat aatgtcaagt cctgtttctt ccgtcagctt ctttgttgcc
     3601 gggatattgg ggcctgaaag catgccgtct ctggaaatat ctgtataaat aatgtgcttt
     3661 acgccatatt ctttcatttt caggcagaga tctgtggctg taacagtgct tacctgctcc
     3721 cagccctgga tagctaccat gccgtccttg gcgtcaacgc ctgctgcaat ggcatctgca
     3781 ccgaatttct ccaccatatc ttttaagaac tccggctctt ctactgcctt tgttcctatg
     3841 atcacacgct ttacgcctaa agcaagcata tttttaatgg cttcctcaga acggataccg
     3901 ccgccgatct ctacagggat attcacagaa gaaacgattt ttttaatggt ctcttcattt
     3961 acagaacgtc ctgccagtgc accgtctaag tctaccagat gtaaaaaagt ggctcctttt
     4021 tcctgccaga gagccgcgat ctcctctgga tgctccgaat ataccgtaat atctttaaat
     4081 gcaccctgac gcaggcggac acattttcca tttttcatat caattgccgg atacagctgc
     4141 atgttttgtc tccttttcta ttcctgaata aatgttttta caggcttttt ctacaggctt
     4201 cccttggtag ataatacatc tgtaagccgg ggatcataac cggttgctcc gtctaaagct
     4261 ttcgcaaatg ccttaaaagc cgcctcaatg atgtggtgat tgttgctgcc ggacatctgg
     4321 cgcagatgaa ggttcatttc cccgccgtaa gatactgcat agaaaaactc ccgcaccatt
     4381 tccgtctcca gatcacctac tttttcccgg tcaatgttta agtcatacac cagataaggt
     4441 cttccgcaaa gatccagggc gcagatgact aaggcctcat ccatgggaag gatctgggaa
     4501 ccatatcggc agatgccttt tttatcccct acggccttgc ggatggcctg tcccagcaca
     4561 atgcctgtat cctcaatggt atgatgggta tccacttcca gatccccgtc tactgtaaga
     4621 tccagatcaa aaaagccatg gcgggcaaag ctgtttaaca tatggtcaaa aaagccgatg
     4681 cctgtatgga tattggcctt tccgctgcca tccaggttta aagtcatgtt tattttcgtt
     4741 tcattggtat ttcgtgtgat ctgcgcaatg cgttccatat ttcctacact ctcctttatg
     4801 cggacctttt tactcatgtt tttggcgagt cccaaggtca aaaatcctcc tgcaagcaag
     4861 cttgcgtcgg atttccgacc ttttgaccct ggtatcattc aaatctgact cttacagaat
     4921 ttgcatgtgc agtcaggtgt tctgcttctg caaagtcctc aatgtcctta tgagcctttt
     4981 caagggcttc tctggaataa tagataatgc tggatttctt aataaaatca tctacaccta
     5041 aaggtgagaa gaattttgct gttccgttgg taggaaggat gtggtttggt cctgcaaaat
     5101 agtctcccaa aggttcagag ctgtattcac cgataaagat tgcgcctgca ttgcggattt
     5161 ttgtcattc
//
LOCUS       DBMDUA010000191         5130 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6219_length_5130_cov_1.861802, whole genome shotgun sequence.
ACCESSION   DBMDUA010000191 DBMDUA010000000
VERSION     DBMDUA010000191.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6219_length_5130_cov_1.861802"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="ACZPCL_11580"
     CDS             <1..355
                     /locus_tag="ACZPCL_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HIR1163142.1"
                     /translation="AVRCCRGWRGSLGALTIEKLEAMTCVCSVGLDMIAIPGDTPSTT
                     IAGIIADEAAIGMVNQKTTAVRLIPVIGKKVGDTAEFGGLLGYAPIIPVNTFSCEKFV
                     TRGGRIPAPIHSFKN"
     gene            368..1000
                     /locus_tag="ACZPCL_11585"
     CDS             368..1000
                     /locus_tag="ACZPCL_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR1163143.1"
                     /translation="MKLYLIRHGRQNSRLCNVNVDLCEEGYRQAALLGERLFPKKIQA
                     VYSSDLLRAVETAQAANLYWNVEHTICPELREIDFGEMEGLSDEEIAVKFADFKKEQA
                     KMETDLAYPGGECAADVVKRADPVLKGLVKTGYERIAVVTHGGLIRVMAAYYTGIPLS
                     RFRILGKDLENCSITELQWDEKNERFTLECFNDHAHLDKYPELLRSGWKG"
     gene            1020..1661
                     /locus_tag="ACZPCL_11590"
     CDS             1020..1661
                     /locus_tag="ACZPCL_11590"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1163144.1"
                     /translation="MKKGIIFDVDGTLWDSSVQVAQSWQVAAEKLLNGPRPITPDMIS
                     RNMGKNMKDFGDALFPDLPPAKRLEVMEACMEYENQYLEDHPGILYPQVAEVLQQLSW
                     KYKLFIVSNCQKGYIEVLMRSCNLEEYITDIECYGNTGLSKADNISMVIQRNHLDQSF
                     YVGDTAMDAEAAADAGIPFVHAAYGFGQVNGAEAVIREFGELLQLSKKLLGED"
     gene            1680..2867
                     /locus_tag="ACZPCL_11595"
     CDS             1680..2867
                     /locus_tag="ACZPCL_11595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1163145.1"
                     /translation="MVGSNEHAQLREALEFCLEKDLERILLSNPSDPDHFSKSKIRPL
                     LLKGNLIFQAEEQKGKQAFHKNLSRDEAVIYVENCLNGPFRQAEITCARGKATVLVSK
                     KGKMTVKFKKQARMINQAALEHNRKKQYLLPEGTAVPFLVDLGVMTKEGAIVHSRYDK
                     YRQINRFLEFIEDILPQLNKNKENVIIDFGCGKSYLTFAMYYYLKEVKGYPVRIIGLD
                     LKEDVIRHCSDLAVRYGYEKLSFTCGDIASYEGVDHVDMVVTLHACDLATDYALEKAV
                     GWGAKVILSVPCCQHELNRQIHNPVLQPVLEYGLLKERMAALYTDGIRAKVLEYRGYN
                     TQILEFIDMEHTPKNILLRAVLRKEGDAEGEKKKETGEQIRELIHFLNADPAIVRLLA
                     PEL"
     gene            complement(2926..4152)
                     /locus_tag="ACZPCL_11600"
     CDS             complement(2926..4152)
                     /locus_tag="ACZPCL_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HIR1163146.1"
                     /translation="MKITGIVAEYNPFHNGHAYQIKKAREVTGCDYCIAVISGDFVQR
                     GEVAVFSKYLRTKMALLCGADLVLEIPSIFAVSSAEDFAAGAVALLDQLGVVTHLCFG
                     SEMGESERFMETAAILADEPDSFSTKLKEGLSEGLSWPQARVRALEAEGISDLGNFLE
                     TPNNLLGVEYCKALIRQKSPIQPITILRKGNGYHSQDLTGELASASAIRREIFKEEPD
                     FSILLSQVPDEIRSLFKAEVPLQPEDLSDLLNYRLLTLKRDGIPFSVFADVSNDLSDR
                     LESKLYEQADWEERIRQLKSRQYTYTRISRCLLHILLGITDELVLAGKEAGFSPYARI
                     LGFRKSKAGVLSEIKKHTRIPLVAKTADASRMLSGTAKMLFEQDLYASHVRQSLLLGK
                     TGQPVRNEFTQPICII"
     gene            4398..>5130
                     /locus_tag="ACZPCL_11605"
     CDS             4398..>5130
                     /locus_tag="ACZPCL_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S1"
                     /protein_id="HIR1163147.1"
                     /translation="MYEEDRENREDRDQEIERTEIMDDVDEQETQTSSSRSQENRKSG
                     SSQNVNWTTPNRSSERTSSERKTQWQESRQQNNRPEHGYRNEYRETVQAPRKKNSWAR
                     KAAGITAAAVLFGTVAGGVMTGVNYVGARLTGLADITATAPAETEGTTTAQVPETSAA
                     SNNGSTTAVSTVTDVSSIAEKAMPSLVAINDTMTVEQNNFFGMPQTYQAQSSGSGIIV
                     GQNDTELLIATNNHVVSGATDMKVTF"
BASE COUNT         1501 a         1056 c         1251 g         1322 t
ORIGIN      
        1 tgccgtccgg tgctgtcggg gatggagagg gtcacttggc gctcttacca ttgaaaaact
       61 ggaagctatg acctgtgtat gctctgtagg tcttgatatg atcgctatcc cgggagatac
      121 accttctact accattgcag gtatcattgc tgatgaggca gccattggta tggtcaacca
      181 gaagactacc gcagtccgcc tgatcccagt gattggaaag aaagtaggag atacagcaga
      241 atttggcggc cttttaggtt atgcaccgat cattcctgtt aatacattct cctgtgagaa
      301 atttgttaca agaggaggac ggatcccggc tccgatccac agctttaaga actaggactc
      361 gatacgtatg aaattatatc tcatccgtca tgggcggcag aacagccgcc tgtgcaatgt
      421 taatgttgat ctttgtgaag agggctaccg tcaggcagcc cttttaggtg agcgtctttt
      481 tcctaaaaag atccaggcgg tttattccag tgacctgtta agagccgtag agacagccca
      541 ggcggctaat ttatactgga atgtggaaca caccatctgt ccggaacttc gtgaaattga
      601 tttcggggag atggaaggtc tttctgatga agagattgct gtaaagtttg cagattttaa
      661 gaaggaacag gcaaaaatgg aaacagatct tgcttatccg ggcggtgagt gtgctgccga
      721 tgtggtaaaa cgggcggatc ctgttttaaa aggccttgta aaaacaggat atgaacggat
      781 cgcagtggta acacacggcg ggctgatccg ggtcatggct gcttattata cgggaattcc
      841 gttaagccgt ttccgtattc ttggaaaaga tctggaaaac tgcagtatta cagaacttca
      901 atgggatgaa aagaatgaac gctttacact ggaatgtttt aatgaccatg cacatttaga
      961 caagtatccg gaacttttaa gaagcggctg gaaaggataa caaaagggga gaataaagga
     1021 tgaaaaaggg aattattttt gatgtagacg gaaccttatg ggattcttct gttcaggtgg
     1081 cccagtcctg gcaggtagcg gcagaaaagc tgttaaatgg accaagaccc atcactccgg
     1141 atatgatcag ccggaatatg ggcaagaaca tgaaagattt cggagacgca ttatttccgg
     1201 atctgcctcc tgcaaaacgc ttagaggtaa tggaagcctg tatggaatat gaaaaccagt
     1261 atctggaaga tcatccggga attctttacc ctcaggtagc agaagttctt cagcagttaa
     1321 gctggaaata taaactgttt attgtaagca actgccagaa gggctatatt gaggttttaa
     1381 tgcgttcctg taatctggaa gaatatatta cagatattga atgttatgga aataccggtc
     1441 tttccaaggc agataatatt tccatggtga tccagagaaa ccacttagat caaagttttt
     1501 atgtaggcga tactgccatg gacgcagagg cagctgcaga tgccggaatc ccctttgtcc
     1561 atgctgccta tggttttgga caggtaaacg gggcagaggc agtgatccgg gagtttgggg
     1621 aacttttaca gctttccaaa aagcttttag gtgaagactg aaaggagaaa aggacaacaa
     1681 tggtaggatc caatgaacat gcacagttaa gggaagcttt ggaattttgc cttgaaaagg
     1741 atttagagag gattctttta agcaatcctt cggacccgga tcatttttca aagtcaaaga
     1801 tccgtccttt gctcctgaag ggaaatctga tcttccaggc agaggagcag aaaggcaaac
     1861 aggcttttca taaaaattta agcagagacg aagctgttat ttatgtagaa aactgcttaa
     1921 atggtccttt ccgtcaggct gagatcacct gtgccagagg caaggcaaca gtacttgtaa
     1981 gtaaaaaagg aaaaatgact gtaaaattta agaagcaggc ccgtatgatc aaccaggctg
     2041 ctttggagca taaccgtaaa aagcagtatc ttttaccgga aggaacagcg gttccgtttc
     2101 tggtagatct tggggttatg acaaaagaag gtgccattgt ccacagccgc tatgataagt
     2161 accgtcagat caaccgtttc cttgaattta tagaagatat tcttcctcag ttaaataaaa
     2221 acaaagaaaa tgtgatcatt gattttggct gcggtaagtc ctatcttacc tttgccatgt
     2281 attattatct gaaagaggta aagggatatc cggtacggat catcggtctg gacctgaaag
     2341 aagatgtgat ccgccattgt tctgacctgg cagtacggta cggatatgag aagctgagct
     2401 ttacctgtgg ggatattgct tcttatgaag gggtggatca tgtagatatg gtagttaccc
     2461 ttcatgcctg tgacctggca acggattatg ccctggaaaa ggcagttggc tggggagcga
     2521 aggtgatcct ttctgttcca tgctgccagc atgaattaaa ccgtcagatc cacaatccgg
     2581 ttctgcagcc tgttttggaa tatggcctgc ttaaggaacg gatggcagct ttgtatacag
     2641 atggcatccg ggcaaaggtt ttagaataca gaggttacaa tacccagatt ttggaattta
     2701 ttgacatgga gcacacaccg aaaaatattc tgctgcgtgc tgtgttaaga aaagaagggg
     2761 atgcagaggg agagaagaaa aaagaaacag gagagcagat acgggaactg atccatttcc
     2821 tgaatgcaga tcccgccatt gtccgtctcc tggctccgga actctgatac tgaattgctg
     2881 tgtgcaagct tgcatcggat ttccgacctt ttgatcctgg tatcatcata taatacagat
     2941 gggctgtgta aactcattac gcaccggctg tcctgttttt cctaaaagca ggctctgacg
     3001 cacatgagat gcatacagat cctgttcaaa taacattttt gctgtaccag aaagcatcct
     3061 ggatgcatcc gcggtctttg cgaccagcgg gatccgggta tgctttttta tttcgcttaa
     3121 aacccctgct ttgcttttgc gaaaacctag gatccgggca tagggggaaa aaccggcctc
     3181 ttttcctgca aggaccagtt catctgtaat gcctaaaaga atatgcagaa gacaccggct
     3241 gatccgggta taggtatatt gcctggattt taactgccgg atccgttctt cccagtctgc
     3301 ctgctcatag agtttgcttt ccaagcggtc tgaaaggtcg tttgatacat ctgcgaaaac
     3361 agaaaaaggg atcccgtccc gctttaaagt caggagtctg taatttagca gatcagacag
     3421 atcttccggc tgcagaggga cttctgcctt aaagagagaa cgtatttcat caggcacctg
     3481 cgataaaaga atggaaaaat ccggttcttc tttaaagatt tctctgcgga tagcggaagc
     3541 ggaggcaagt tctcctgtaa gatcctggct gtggtatcca tttccctttc gtaaaatagt
     3601 aatcggctgg atgggacttt tctgccggat cagggctttg cagtattcta cacctagcag
     3661 attgttaggc gtttccagga agtttccaag atccgatatt ccttctgctt ctaaagcccg
     3721 gactcttgcc tgaggccagg aaagcccctc tgaaagtcct tcttttaatt ttgtggaaaa
     3781 tgagtccggc tcatctgcaa gaatagccgc tgtttccata aaacgttcac tttctcccat
     3841 ttcactgcca aaacaaagat gggtcaccac tcccagctga tcaagaaggg caactgcacc
     3901 ggctgcaaag tcctctgcac tgcttactgc aaagatggaa gggatctcaa ggaccaggtc
     3961 tgcgccgcac agaagtgcca tctttgtacg caggtattta ctgaaaacag ccacttctcc
     4021 ccgctgtaca aaatctccgc ttataacggc gatgcagtaa tcacagccgg ttacttcacg
     4081 ggcttttttg atctgatagg cgtgtccgtt atggaatgga ttatattcag ctacaatacc
     4141 ggttatcttc atcttatttt cgtctcctgt cctaagtctg tgtatacaga atggcgtatt
     4201 catggtacta ttatacccac ggacagaagg ttcgtccagc atgccttaag atcaggacac
     4261 aaaatataca caattgcatc acaattgcat caaaaacaga gggtatattg tagtcatcaa
     4321 aaggaaagag aaagaaatta aaaagaaaat aaaaatccat ttgaaatata caaaggcatc
     4381 tgaaaggagc gttttttatg tacgaagaag atagagaaaa cagagaagat agagatcagg
     4441 agatcgaaag aacagaaatt atggatgatg tagatgagca ggaaactcaa accagttcat
     4501 cccggtcaca ggaaaacaga aagtccggtt cttcacagaa tgtaaactgg actaccccaa
     4561 accgttcttc tgaaagaact tcctctgaga gaaaaactca gtggcaggag agcagacagc
     4621 agaataacag accggaacat ggttacagaa atgaataccg tgaaaccgtt caggcaccaa
     4681 gaaagaaaaa cagctgggca agaaaggcag caggtattac tgcagcagct gtgctgtttg
     4741 gcacagtagc aggcggtgtg atgacaggtg ttaattatgt aggcgcccgt cttacaggac
     4801 ttgctgatat aacagctact gcaccggcag aaacagaagg aaccaccact gcacaggtac
     4861 cggaaacaag cgctgcatcc aataacggaa gtacgaccgc tgtatctaca gtaaccgatg
     4921 tttcttccat cgctgaaaag gcaatgcctt ctctggtagc catcaacgat accatgactg
     4981 tggaacagaa caatttcttt ggtatgcctc agacttatca ggcacagagc agcggttctg
     5041 gtatcattgt tggacagaat gatacagaac ttctgatcgc taccaacaac cacgttgtat
     5101 ccggtgcgac agatatgaag gttaccttta
//
LOCUS       DBMDUA010000192         5049 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6324_length_5049_cov_2.191188, whole genome shotgun sequence.
ACCESSION   DBMDUA010000192 DBMDUA010000000
VERSION     DBMDUA010000192.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6324_length_5049_cov_2.191188"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(107..1396)
                     /locus_tag="ACZPCL_11610"
     CDS             complement(107..1396)
                     /locus_tag="ACZPCL_11610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1163148.1"
                     /translation="MKTLQMIHYFNFVIATVFVVFYFYQIGYLLLGLLMKHRKNKTGS
                     HQFHRYAAVISARNEANVIGGLIASLKAQNYPSDLLDIYVIADNCTDNTAQVAAQAGA
                     RVYRRYNHQQVGKGYALDYLFKCLSASGKDVYDGYFVFDADNYVDPDFVKEMNATFDS
                     GHFAALTSYRNSKNFGANWISAGYGLWFLREARFLNFPRMLLGTNCAISGTGFLVSGE
                     VIRENGGWPFHLLTEDIEFSVNCAIEGKVIGYCDKAVIYDEQPLKFGQSWTQRLRWSK
                     GFYQVDWHYSWRLIKGFFQGGRKSFSCYDIFMTVAPGMFFTLAAILINLGMAFSYMTE
                     PGYIARMIAAENISFLTRTIVMFYLSLLVYGAITMISEWKQVQLSALKKILYTFTFPI
                     FMFTYIPISAVALVKKVEWKPICHGNTSGELITSKRN"
     gene            1671..2093
                     /locus_tag="ACZPCL_11615"
     CDS             1671..2093
                     /locus_tag="ACZPCL_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163149.1"
                     /translation="MLNEEKIQAMTDLAVFEKHQGRKIFPVNQYFKSDYVGGQLFRSF
                     FGYTFSFALILLMWILYKLDVLMKGADIDQLIGWIRNWGLAYVTGLGGYLLITWKIYS
                     RRYDFASRSQTMYVARLKHLDRKFGKDRGKENRGGAAL"
     gene            2090..3073
                     /locus_tag="ACZPCL_11620"
     CDS             2090..3073
                     /locus_tag="ACZPCL_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1163150.1"
                     /translation="MTVLLLWKEHLKEIYNKYSYVIRFVVRLLLILCTILSMNANIGY
                     MAKLKNPVVVALLCFFGALLPDSGAVFLIGCVMVAHVYAVSMEMALITLMMILVVAVL
                     YYGFKPGDSWLMILTPLAFLFKVPYAVAFLVGLGGSLISVIPVSCGVFLYYLLMYIRQ
                     NAGVLTGEGSVDIVQRYSQIIRSVCFNQTMMVMIAACAVGIMVVYLIRRLSVDYAWII
                     AIVAGTVAQLLVIFVGDFVFGVSVSAGTLIFGLLVSEMLAMIYNFFFFSVDYTRTEYV
                     QFEDDDYYYYVKAIPKMAVSSTDVKVQRISGRKRNTREKNTARNTAERELR"
     gene            3152..4006
                     /gene="cdaA"
                     /locus_tag="ACZPCL_11625"
     CDS             3152..4006
                     /gene="cdaA"
                     /locus_tag="ACZPCL_11625"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR1163151.1"
                     /translation="MMKASSWLTLLPRISLRNLFEIAIISFLVYEILFWIKNTRAWTL
                     LKGLLTIMVFAIVAVLLRLDTIVWILKNITTIAVTAFLVVFQPELRKALEQLGSQNLL
                     SGILAQDDGKSSQEFNDRTVNELVRATFEMAKVKTGALMVIERGTSLKEIERTGIEIS
                     GLVTSQLLINIFEHNTPLHDGAVVIRGNRVAAATCYLPLSDNMSINKALGTRHRAAVG
                     VSETTDSLTIVVSEETGRVSVAEGGMLRSVPTAEALRNILSGLMKEEPVSSNRFKIWK
                     GRDQWEKS"
     gene            3991..>5049
                     /locus_tag="ACZPCL_11630"
     CDS             3991..>5049
                     /locus_tag="ACZPCL_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR1163152.1"
                     /translation="MGKKLTNNLKLKILSVFLAFFVWLAVTNISNPDVTATKEVPLEV
                     LNEDVLSTNGKTYELIDNRTTVTVAYKVRTLDAGSISASDFRAYIDLADMYEPTGAVP
                     VKVDVKNNKVDAVTAKPMVIRVTTEDVQQKKFDLTAYTEGKTESGYREGTVNIVPTSI
                     YVSGPESQVGRISTVGITIRLDGANSDLSGTAQIQCFDANRNKITLDDRVTLSRSEAD
                     YTLSILKVKTLGLNFETEGRVADGYRFTGIESNVNSVDVVGLKSDLAEINAINIPKSE
                     LNMDGASADKEVIIDLNKYLPENVELADSNSKIHVTLKVEPLETRTIELKTSKIRQVG
                     ASSRYSYQYDRDAIRLSIK"
BASE COUNT         1515 a          984 c         1188 g         1362 t
ORIGIN      
        1 ttcataagac tattatgaga tagattttag aaaaaagaaa cccctaaccc ctcaagattg
       61 aggggcaggg gagttgaata ggcgaagcct attttttata tttaatttaa ttcctcttgg
      121 aagtgatcaa ctctccggaa gtgtttccat gacagatcgg cttccattct actttcttaa
      181 ccagtgcaac tgcagagatc gggatgtaag tgaacatgaa gatcgggaat gtaaatgtat
      241 aaaggatctt ctttaatgca gacagctgca cctgtttcca ctcactgatc atggtgatgg
      301 caccataaac cagcagactt aagtagaaca taacaatggt ccttgtaagg aaggaaatat
      361 tctcagctgc gatcattctt gctatgtaac caggttctgt catataggaa aatgccatgc
      421 ccaggttaat aaggatagcg gcaagagtaa agaacatacc aggtgctacg gtcatgaaaa
      481 tatcatagca ggaaaatgat tttcttccgc cctggaaaaa tcccttgatt agtctccagc
      541 tgtaatgcca gtcaacctga taaaatcctt tggaccaacg cagtctctgg gtccaggact
      601 gaccaaattt taaaggctgc tcatcgtaga tcacagcctt atcacagtag ccgatgacct
      661 tgccttcaat ggcacagtta acggaaaatt caatatcctc tgtaagaaga tggaatggcc
      721 agccgccgtt ttcgcggatc acttcaccgg ataccagaaa gccggttccg gaaatagcac
      781 agttggttcc cagaagcatt cttgggaagt ttaagaaacg ggcctcccgc aggaaccata
      841 agccgtaacc agctgagatc cagttggcac cgaagttctt ggaattacgg tagcttgtaa
      901 gagcagcaaa atgtccgctg tcaaaggtgg cattcatttc tttgacaaaa tcaggatcta
      961 cataattatc tgcatcaaat acaaagtagc cgtcatatac atctttaccg gaggcagaaa
     1021 gacatttgaa cagatagtca agggcataac cttttcccac ctgctgatgg ttgtaacggc
     1081 ggtacaccct tgctcctgcc tgtgcagcaa cctgcgcggt attatctgtg cagttatctg
     1141 cgatcacata aatgtcaaga aggtcagatg gataattctg agcctttaaa ctggcaatca
     1201 gaccaccgat gacatttgct tcatttcttg cagaaataac agcagcgtaa cggtgaaact
     1261 gatgggaacc ggttttattt ttcctgtgtt tcataagaag ccccaggagc agataaccga
     1321 tctgataaaa ataaaagact acaaatacag ttgcgatcac aaaattaaaa taatggatca
     1381 tctgaagtgt tttcataata tacctctttg taatacttga aatatttgac aaattaataa
     1441 tatttctgat tttagcgtga tctttttcgc agaatacaaa atagcatatt ttaagaataa
     1501 tgcaagtctt gacaaagaaa ttcataaaaa attaataatt gcttcataca atattcaatt
     1561 atgaacggta ttaaaaagac ttcctaaaaa ttcatttaag gtgtcgcaaa acaaaaaaag
     1621 atgtggtata ctaaattgat agcattggga agaagaaaaa ggagtgcttt atgctgaatg
     1681 aagaaaaaat ccaggcaatg acagatctgg cagtgtttga aaagcatcag ggcagaaaaa
     1741 tcttccctgt aaatcaatac tttaaaagtg attatgtagg agggcagttg ttccgttctt
     1801 ttttcgggta cacctttagt tttgcgctga tccttcttat gtggatcctt tataaactgg
     1861 atgtgctcat gaagggagct gatattgatc agctgatcgg ctggatccgc aactggggac
     1921 tggcctatgt gaccggactg gggggatatc ttcttattac atggaagatc tattccagac
     1981 ggtatgattt tgcatccaga agccagacca tgtatgtggc caggcttaaa catctggacc
     2041 gtaaatttgg aaaggaccgg ggaaaagaga acagaggagg agccgcttta tgacagttct
     2101 tttgctgtgg aaagagcacc tgaaagaaat ttataataaa tacagctatg tgatccgctt
     2161 tgtggtccgt ctgctgttga tcctgtgtac gatcttaagt atgaatgcca atatcggcta
     2221 tatggcaaaa ttgaaaaatc cggtcgttgt agcgctgctg tgcttttttg gagccttgct
     2281 tccggacagc ggggccgtct tccttatagg atgtgtgatg gtagcacatg tgtatgcagt
     2341 atccatggaa atggcactga tcacactgat gatgatcctg gtagtggctg ttctttatta
     2401 tgggtttaag ccgggagaca gctggcttat gatactgaca ccccttgcct tcttatttaa
     2461 ggtgccttat gcggttgctt ttttagtagg gctgggaggc agtcttattt ctgtaatacc
     2521 tgtaagctgc ggagtctttt tatactatct tcttatgtat atacggcaga acgcaggcgt
     2581 ccttacagga gagggcagcg tagatatcgt acagcgttac agtcagatca tccggtccgt
     2641 atgctttaac cagacaatga tggtgatgat cgctgcctgt gcagtaggga tcatggtggt
     2701 atacctgatc cgcaggctgt ctgtagatta tgcctggatc atagcgatcg tagctggaac
     2761 agtagcacag cttttggtga tctttgtagg agattttgtt ttcggggtat ccgtatctgc
     2821 aggaacgctg atcttcgggc tgcttgtttc tgagatgctg gctatgatct acaatttctt
     2881 tttcttctct gtagattata cccgtacaga atatgtacag tttgaggatg acgattatta
     2941 ttattatgta aaagcgatcc ccaagatggc agtaagcagt accgatgtaa aagtacagcg
     3001 gatcagcggg agaaagcgga atacaagaga gaaaaataca gccaggaata cggctgaaag
     3061 agaattaaga taaatgatca agataaataa ttaagataac gataattaga ctaaaggagg
     3121 atgatataag gtgggaatct taacagaact tatgatgaaa gcctcatcct ggctgacgct
     3181 attgccaagg atctccctcc ggaatttgtt tgagatagcg attatctcat tcctggtata
     3241 tgagatcctt ttctggataa aaaatacaag ggcatggacc ttactgaagg gacttttaac
     3301 gatcatggta tttgccattg tggcggtcct tttacgtctg gataccattg tctggatcct
     3361 taaaaatatt acaacgatcg cagtaacggc atttctggtc gttttccagc cggaactgcg
     3421 aaaagcactg gaacagttag gcagccagaa cctgttatcc ggtattctgg ctcaggatga
     3481 cggaaagagc agtcaggaat ttaatgaccg cacagtcaat gaactggtac gtgctacttt
     3541 tgagatggca aaggtgaaaa caggagcctt aatggtcatt gaaagaggaa cttccttaaa
     3601 ggagatcgag aggaccggta ttgagatcag cggactggtc acaagccagc ttcttataaa
     3661 tatttttgaa cacaatacac ctcttcatga cggagcggtg gtgatccgcg gaaaccgggt
     3721 ggcagcggct acctgttacc tgcctctttc tgacaatatg tctattaata aggcattggg
     3781 aaccagacac cgtgcagcag taggtgtcag cgagacaaca gacagcctta ccattgttgt
     3841 ttctgaggaa acaggacggg tatctgttgc agaaggagga atgctccgca gtgttccaac
     3901 agcagaggca cttcggaata ttctttccgg actgatgaaa gaagagccgg tttcttccaa
     3961 ccggtttaag atatggaagg gaagggatca atgggaaaaa agctgacaaa taacttaaaa
     4021 ctaaagatac tttccgtttt ccttgccttt tttgtgtggc tggcagtgac caacatttcc
     4081 aacccggatg taactgctac caaggaagta cctttggagg tgctgaacga agacgtgctt
     4141 tccacaaacg gaaaaaccta tgaactgatc gacaaccgca cgacagtgac ggtagcatac
     4201 aaagtgcgta cactggatgc aggaagtatt tctgcttctg acttccgggc ctatatcgat
     4261 ctggcagata tgtacgagcc tacaggagca gttcctgtta aggtagatgt aaagaataat
     4321 aaagtagatg ctgtaacagc aaaacctatg gtgatccgtg tgactacgga agatgtacag
     4381 cagaaaaagt ttgacctgac tgcctataca gagggaaaga ccgagtccgg gtacagagag
     4441 ggaaccgtca atatcgtgcc caccagtatt tatgtaagcg gacctgaatc acaggtagga
     4501 cggatcagta ccgttggtat caccattcgt ttagacggtg caaactctga tctttccggt
     4561 acagcccaga tccagtgctt tgatgcgaac cgcaataaga tcactctgga tgatcgtgtg
     4621 actttaagcc ggtcagaggc agattacaca ctttctatct taaaagtaaa gactctgggg
     4681 ctgaattttg agacagaggg aagagttgca gacggatacc gttttacagg gatcgagagt
     4741 aatgtaaatt ccgtagatgt agtaggactt aagtctgatc tggcagagat caatgccatc
     4801 aacattccta agtcagagtt aaatatggac ggtgcttctg cagacaaaga agtgatcatt
     4861 gatttaaaca aatatcttcc ggaaaatgta gaactggcag actctaacag caagatccat
     4921 gtgactttaa aggtagaacc tttagagacc aggaccattg agttaaaaac aagcaagatc
     4981 cgccaggtag gcgcttccag ccgttacagt taccagtatg acagagatgc tatccgttta
     5041 agcatcaag
//
LOCUS       DBMDUA010000193         4993 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6401_length_4993_cov_2.305805, whole genome shotgun sequence.
ACCESSION   DBMDUA010000193 DBMDUA010000000
VERSION     DBMDUA010000193.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6401_length_4993_cov_2.305805"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..3484
                     /gene="nifJ"
                     /locus_tag="ACZPCL_11635"
     CDS             <1..3484
                     /gene="nifJ"
                     /locus_tag="ACZPCL_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR1163153.1"
                     /translation="YAFTDVAAIYPITPSSPMAEATDEWATDGRTNIFGHEVQITEMQ
                     SEAGAAGAVHGSLAAGALTTTYTASQGLLLMIPNLYKIAGEQLPGVFNVSARALASHA
                     LSIFGDHSDVYACRQTGCAMLCESSVQEVMDLTPVAHLAAIKGKVPFINFFDGFRTSH
                     EIQKIETWDYEDLKDMADMDAIAEFRKHALNPNHPCQRGSAQNPDIFFQAREACNPYY
                     DALPAIVQEYMDKVNAKLGTDYKLFNYYGAADAEHVIIAMGSVNDTIEETIDYLTKQG
                     KKVGVVKVRLYRPFCAQALIDAIPETVKTISVLDRTKEPGAIGEPLYLDVVAALKGSK
                     FDQVKILTGRYGLGSKDTTPAQIVAVYENEAKTPFTIGIVDDVTNLSLEIGAPLVTTP
                     EGTTNCKFWGLGADGTVGANKNSIKIIGDNTDMYAQAYFDYDSKKSGGVTMSHLRFGH
                     SPIKSTYLIHKANFVACHNPSYVRKYNMVQELVDGGTFLLNCAWDMEGLEKHLPGQVK
                     KFIADHKINFYTIDGVKIGIETGMGPTRINTILQSAFFELTGIIPAEKANELMKAAAK
                     ATYGRKGEDVVMKNWAAIDAGAKGVHKVEVPASWANCEDEGLDYKVVTEGRKEVVDFV
                     NNIQTKVSAQEGNTLPVSAFKDYVDGSTPSGSSAYEKRGIAVKVPVWNVDNCIQCNFC
                     SYVCPHAVIRPVAMTAEEAAKAPADMKMKDMTGMPGYKFSMAISALDCTGCGSCANVC
                     PGMKGNKALEMKPLEASLSEQANYDFAETLPVKEEVVAKFKENTVKGSQFKKPLLEFS
                     GACAGCGETPYAKLITQLFGDRMYIANATGCSSIWGNSSPSTPYTMNAKGQGPAWDNS
                     LFEDNAEFGYGMLLAQNAIRDGLKSKVESVMANENASEEVKAACKEWLDTYAVGALNG
                     TATDKLVAALDGIDCPTCKEIVANKDFLAKKSQWIFGGDGWAYDIGFGGVDHVLASGK
                     DINIMVYDTEVYSNTGGQASKSTKTGAVAQFAAGGKETKKKDLAGIAMSYGYVYVAQI
                     CMGGDMAQTVKALAEAEAYPGPSLIIAYAPCINHGIKKGMDKAMTEEKLALECGYWNN
                     FRYNPAAEKKFTLDSKAPKMEGYQDFLKGEVRYLSLTMKNPDRAATLFARNEKEAKER
                     YEHLSKLVALYGND"
     gene            3578..4579
                     /locus_tag="ACZPCL_11640"
     CDS             3578..4579
                     /locus_tag="ACZPCL_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1163154.1"
                     /translation="MMAEKLTMADIAELAGVTKSTVSRYFNGGSIKEETRKKIQNVVE
                     ENNYIPNSFARLKAKESNTVGVIVPTLNSKVTSRVVTSIGRYLRSQGYEALIKDSDHS
                     PKLELENINRLFTQKVDGILFSAISLTEEHKQLLMNSPVPVVVLAQNFEEGITVTMDD
                     YTAGKTMGSFMGSRVHGKIAYLGVDEEDEAVGIFRRQGVLEGIKESGSQVMTVETGDY
                     SYVSGQEMMEKVLEKGIPDGVICATDRLAFGAYRILQKHGILIPEQVSVAGFGGYDES
                     ELLSPQLTTLRFDSYGLGYLGAETLLKMIREEPVPKKQIVGFEMIPGKSVRNENTVK"
BASE COUNT         1480 a         1028 c         1244 g         1241 t
ORIGIN      
        1 atatgcattt actgatgtag cggctattta tccgattacc ccatcttcac ctatggctga
       61 agctacagat gagtgggcaa cagacggaag aaccaatatt tttggtcatg aagttcagat
      121 cacagagatg cagtctgagg ctggtgcagc aggtgctgta cacggttctc tggcagcagg
      181 tgctttaacc accacctaca ccgcttccca gggtctgtta ctgatgatcc ctaaccttta
      241 taagatcgct ggtgagcagc tgccaggcgt atttaacgta tctgcccgtg cacttgccag
      301 ccatgcctta tctattttcg gtgaccactc cgacgtttat gcctgtcgtc agactggatg
      361 cgcaatgctg tgcgagtcca gtgtacagga agttatggac ttaaccccag ttgctcatct
      421 ggcagctatc aagggcaaag ttcctttcat caacttcttc gacggtttcc gtacatctca
      481 tgagatccag aagatcgaga cctgggatta cgaagatctg aaggatatgg ctgatatgga
      541 tgcgattgct gaattccgta agcatgcatt aaatccaaat catccatgcc agagaggttc
      601 tgctcagaac ccagatatct tcttccaggc aagagaagct tgtaacccat actatgatgc
      661 tcttccagca atcgttcagg agtacatgga caaggttaac gctaagcttg gtacagatta
      721 taagctgttc aactactatg gtgcagcaga cgcagagcat gttatcatcg ctatgggttc
      781 tgtaaatgat accattgaag agaccatcga ctacttaaca aagcagggca agaaggtcgg
      841 cgttgttaag gttcgtctgt acagaccatt ctgtgcacag gctctgatcg atgctattcc
      901 agaaactgtt aagactatct ccgtattaga cagaacaaaa gagccaggcg ctattggcga
      961 gcctctgtac ctggatgttg ttgctgctct taagggaagc aagtttgatc aggttaagat
     1021 tttaaccgga cgttacggtt taggttccaa ggataccaca cctgctcaga tcgtagcagt
     1081 atatgagaat gaagctaaga ctccattcac catcggtatc gttgatgatg ttaccaatct
     1141 gtctcttgag atcggtgctc cattagtcac tactccagaa ggaactacta actgtaagtt
     1201 ctggggtctg ggtgctgacg gtaccgtagg tgctaacaag aactccatca agatcattgg
     1261 tgataataca gatatgtatg ctcaggcata ctttgattat gactctaaga agtccggcgg
     1321 tgttacaatg tctcacctgc gtttcggaca cagcccgatt aagtctacct acttaatcca
     1381 caaggctaat ttcgtagcat gtcataatcc atcctatgta cgtaagtaca acatggtaca
     1441 ggagctggtt gacggcggta ctttcctgtt aaactgcgca tgggatatgg aaggccttga
     1501 gaagcactta ccaggccagg ttaagaagtt cattgctgat cacaagatta acttctacac
     1561 catcgatggt gttaagatcg gtattgagac cggtatggga ccaacacgta tcaacacaat
     1621 cttacagtcc gcattcttcg agctgaccgg aatcatccca gctgaaaagg ctaacgagct
     1681 gatgaaggct gctgcaaagg caacttacgg acgtaagggt gaagacgttg ttatgaagaa
     1741 ctgggcagct atcgatgctg gtgccaaggg cgttcacaaa gtagaagttc cagcaagctg
     1801 ggctaactgc gaagatgaag gtcttgatta taaggtagtt accgagggaa gaaaggaagt
     1861 tgtagacttc gtaaacaaca tccagaccaa ggtaagcgct caggaaggta acacactgcc
     1921 tgtatctgca ttcaaggatt acgttgatgg ttctacacca tccggttctt ctgcatacga
     1981 gaagcgtggt atcgctgtta aggttcctgt atggaacgtt gataactgta tccagtgtaa
     2041 cttctgttct tatgtctgcc cacacgctgt tatccgtcca gtagctatga ctgctgagga
     2101 agctgctaag gctcctgcag atatgaagat gaaggatatg actggtatgc caggatacaa
     2161 gttctccatg gctatctctg ctctggattg taccggatgc ggttcctgtg ctaacgtttg
     2221 cccaggcatg aagggtaaca aggctctgga aatgaagcca ttagaggcta gtctgtctga
     2281 gcaggctaac tacgacttcg ctgagactct gccagttaag gaagaagttg ttgctaagtt
     2341 caaagagaac actgttaagg gaagccagtt caagaaacct ctccttgagt tctctggtgc
     2401 atgtgcaggt tgtggtgaaa ctccatacgc caagctgatc acccagctgt ttggtgacag
     2461 aatgtacatt gcaaacgcaa ctggatgctc ctctatctgg ggtaactctt caccatctac
     2521 accttacacc atgaacgcta agggtcaggg tcctgcatgg gataactccc tgttcgagga
     2581 caacgctgag tttggttacg gtatgttact ggctcagaac gctatcagag atggcttaaa
     2641 gtccaaggtt gagtctgtaa tggctaacga gaacgcttct gaagaagtta aggctgcttg
     2701 caaggaatgg ttagatacct acgctgtagg cgcattaaac ggaactgcaa ctgacaagtt
     2761 agttgctgca ttagacggca ttgactgccc aacctgcaaa gagatcgtag ctaacaagga
     2821 cttcttagct aagaaatccc agtggatctt cggtggtgac ggatgggctt acgatatcgg
     2881 tttcggcggt gtagaccatg ttctggcttc cggaaaagat atcaacatca tggtttacga
     2941 taccgaggtt tactccaata caggtggtca ggcttccaag tctactaaga ccggtgctgt
     3001 agctcagttt gctgcaggcg gtaaagagac caagaagaaa gaccttgctg gtattgcaat
     3061 gagctatggt tatgtatatg ttgctcagat ctgtatgggt ggcgatatgg ctcagactgt
     3121 taaggctctg gctgaagcag aagcatatcc aggaccatcc ctgatcatcg cttatgctcc
     3181 atgtatcaac catggtatca agaagggcat ggacaaggct atgaccgaag agaagctggc
     3241 tcttgagtgc ggatactgga acaacttccg ttacaaccca gctgctgaga agaagttcac
     3301 cttagattct aaggctccta agatggaagg atatcaggac ttcttaaagg gcgaggttcg
     3361 ttacctgtcc ttaaccatga agaacccaga cagagcagct actctgttcg cacgtaacga
     3421 gaaagaagct aaagagcgtt acgagcatct gtctaagctg gttgcacttt atggaaatga
     3481 ctaattcatc ctgcaaagtg agttagaata gattaggtta aatcaacccc ggagggcgta
     3541 aaaactctcc ggggttttta aaaagaaagg gaaatgcatc atggctgaaa agctgacaat
     3601 ggctgatatt gcagaactgg cgggagttac caaaagtacg gtatcccggt attttaacgg
     3661 gggaagcatc aaagaagaga cgagaaaaaa gatccagaat gtggtagaag agaataacta
     3721 tatcccaaac agcttcgcaa ggttaaaagc caaagaaagc aatacagttg gtgtgatcgt
     3781 ccctacttta aactcaaaag tgaccagccg cgtggtcaca agcattggcc gttatctgag
     3841 aagtcaggga tatgaggctc tgatcaagga ctcagatcac agcccaaagc tggaactgga
     3901 aaatatcaac cgccttttta cccaaaaagt agacggtatt ttattcagtg ccatttctct
     3961 taccgaggaa cataagcagc ttcttatgaa ttcgccggtg ccggtagtag ttcttgccca
     4021 gaattttgaa gagggcatta ctgtaactat ggacgattac acagcaggaa agaccatggg
     4081 aagttttatg ggaagcaggg tccatggaaa gatcgcttat ttaggtgtag atgaagaaga
     4141 cgaggcagtg ggaattttcc gccgccaggg agtcttagag gggatcaaag agagcggatc
     4201 ccaggtgatg accgtagaaa caggagatta tagctacgta agcggccagg aaatgatgga
     4261 aaaagtcctt gagaaaggaa ttcctgacgg tgtgatctgc gctacagacc gtctggcttt
     4321 tggagcatac cgtatattgc aaaagcatgg cattttaatt ccggagcagg tatcagtagc
     4381 cggttttggc ggctatgatg aaagcgaatt gttgtctcca cagcttacca ccctgcgttt
     4441 tgactcctat ggactgggct atctgggagc ggagactctt cttaagatga taagagagga
     4501 accggtacca aaaaaacaga tcgtagggtt tgaaatgata cctggaaaaa gtgtgagaaa
     4561 tgaaaataca gtaaaataaa cgggaaatag acagggcaga accgggccaa aaagggtcca
     4621 aaagcaaaga aaagtaataa aaaggtcgtg tttgtgcaag ttgcataaaa atgaccttta
     4681 tttttttaca ctcaacaatg gaaccggtac cataaataac attgacatta gacacaaaaa
     4741 ggtatatgat acaaatatgg aaccggtacc aatcacaaac aggggagata gttacaatag
     4801 agacagaaaa acaaagattg gtaccggata taccacaaag tgtggtcata tatcacaata
     4861 aataagagtt gtgtgaaatg tctccggaac aaaaaatccg agagaacatt aaaaagaaag
     4921 ggaaggtaaa aaatggatta cgcaaaaatt gcagaacagg taattcagta tgtgggcggt
     4981 aagaaaaaca tca
//
LOCUS       DBMDUA010000194         4914 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6491_length_4914_cov_2.160191, whole genome shotgun sequence.
ACCESSION   DBMDUA010000194 DBMDUA010000000
VERSION     DBMDUA010000194.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6491_length_4914_cov_2.160191"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(89..319)
                     /gene="acpP"
                     /locus_tag="ACZPCL_11645"
     CDS             complement(89..319)
                     /gene="acpP"
                     /locus_tag="ACZPCL_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1163155.1"
                     /translation="MEFEKLQGIIAEVLNIEPEDVTMAATFVDDLGADSLDIFQIIMG
                     IEEEFDIEIPNEAAEQIVSVGDAVEQIKNALN"
     gene            complement(345..1370)
                     /gene="plsX"
                     /locus_tag="ACZPCL_11650"
     CDS             complement(345..1370)
                     /gene="plsX"
                     /locus_tag="ACZPCL_11650"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR1163156.1"
                     /translation="MIKVAVDAMGGDLAPVEMVAGAVEAVNAKPGIQVLLVGQEAVVN
                     KELSKYTYNKEQIQVVNATEVIATEEPPVNAIRKKKDSSIVVGMNLVKNKEADAFVSA
                     GSSGAILVGGQVIVGRIKGVERPPLAPLIPTEKGVSLLIDCGANVDARPSHLVQFAQM
                     GSIYMEHVVGIKNPRVAIVNIGAEEEKGNALVKETFPLLKACPDINFIGSIEAREIPH
                     GGADVIVCEAFTGNVILKLYEGVGATLIHMVKQGMMATLRSKIGALLVKPALKTTLKA
                     FDASQYGGAPLLGLKGLVVKTHGSSKRTEVRNSIIQCVTFKEQEINEKIKESLTVGNG
                     KTEEMKE"
     gene            complement(1554..1736)
                     /gene="rpmF"
                     /locus_tag="ACZPCL_11655"
     CDS             complement(1554..1736)
                     /gene="rpmF"
                     /locus_tag="ACZPCL_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR1163157.1"
                     /translation="MSICPKNKSSKARRDSRRANWKMSAPNLVKCSKCGALMMPHRVC
                     KACGSYNKREIVNTAE"
     gene            complement(1740..2267)
                     /locus_tag="ACZPCL_11660"
     CDS             complement(1740..2267)
                     /locus_tag="ACZPCL_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR1163158.1"
                     /translation="MLINLSELFPVEGKSKTYTVPLEMTHFKGPDGSYKVVEKEPVTL
                     VITNQGERVLTVTGKAVLSLEMPCARCLEPVLVPFELEIDQELDMNQTKEDRIEALDE
                     QFYINGYNLDVDQLVGNELTLNLPMRVLCSDNCKGICNRCGTNLNRGTCDCDNRSLDP
                     RMSVIQDIFKQLKEV"
     gene            complement(2343..3539)
                     /locus_tag="ACZPCL_11665"
     CDS             complement(2343..3539)
                     /locus_tag="ACZPCL_11665"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009429378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIR1163159.1"
                     /translation="MKILVINCGSSSLKYQLIDMDNESVVAKGLCERIGIEGSKLTHQ
                     PAGKDKYVVESPMPNHTVAINMVMEALQDKDHGVIASTDEISAIGHRVLHAGVVYSES
                     VVVNEDVKRVIRECFDLGPLHNPANLMGIEACEQAMPGKPNVAVWDTGFGMSMPEKAY
                     MYAIPHEYYEKYSIRRYGFHGTSHMFVSGEAIKFGNLDPEKAKVIVCHLGNGASVSAS
                     IGGKCVDTSMGLTPLEGLIMGTRSGDVDPAVVQFICNKEGKDVNEVLNILNKKSGVLG
                     MSGGISSDFRDIEKAKEEGNHLAAVALDAFVYRVAKYVGAYTAAMNGVDAIAFTAGVG
                     ENDKVTRKNVCEYLGYLGVKIDDEANNVRGERRIISAPDSKVKVMLIPTNEELAIARE
                     TLRLVK"
     gene            complement(3642..4637)
                     /gene="pta"
                     /locus_tag="ACZPCL_11670"
     CDS             complement(3642..4637)
                     /gene="pta"
                     /locus_tag="ACZPCL_11670"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HIR1163160.1"
                     /translation="MAVIDIIKEKAKADKKTIVLPESMDRRTWEAAEKILKEGLANLI
                     IIGTPEDIGEHSKGLDVSGATIINPQTYEKTQEYIDLFVELRKAKGMTPEKAKETILG
                     DYAYYGCLMIKNGDADGMVSGACHSTANTLRPCLQVIKTKPGTKLVSAFFLMEVPDCD
                     LGDNGTFVFADCGLNQNPTPEELAAIAVSSAESFKMLVGHEPKVAMLSHSSKGSAKHA
                     DVDKVVEATKLAKEMAPELALDGELQLDAAIVPEIGASKAPDSKVAGHANVLVFPDLD
                     AGNIGYKLVQRLGKAAAYGPMCQGIAKPVNDLSRGCSADDIVGVVAITAVQCQNN"
     gene            complement(4796..>4914)
                     /locus_tag="ACZPCL_11675"
     CDS             complement(4796..>4914)
                     /locus_tag="ACZPCL_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HIR1163161.1"
                     /translation="NMPYISGTAMVNYATMPMTVEVGGATIFVINVDHYEKI"
BASE COUNT         1205 a         1234 c          962 g         1513 t
ORIGIN      
        1 aataaacaaa atgctggtat ttacgcaagt ccgtaccagc tgtaaaacgc acaaaaaggg
       61 ctgtgcccac acagcccctg tgccatcatt aatttaatgc gttcttaatc tgctctactg
      121 cgtcgccaac actcacaatc tgttctgcag cttcatttgg aatctcaatg tcgaactcct
      181 cttcaattcc catgatgatc tggaagatat ccagagagtc agcacccagg tcatctacaa
      241 atgtagctgc cattgtcaca tcctccggtt caatgttcaa cacttctgca ataattccct
      301 gcaatttttc aaattccatt tcttagctcc tctttcttag atctttattc tttcatttct
      361 tctgtttttc cgtttccaac cgttaaggac tctttgatct tttcattgat ttcctgttcc
      421 ttaaaagtaa cgcattggat gatggagttg cgtacctctg tacgctttga acttccgtgg
      481 gtcttgacta ccagaccttt taagcctaac aatggcgctc cgccgtactg gctggcatca
      541 aatgccttta atgtggtctt caaagctggt tttactaata gagcaccgat cttgcttctt
      601 aaggtggcca tcatgccctg cttcaccata tggatcagcg tagcacccac accctcgtaa
      661 agcttaagga tcacattgcc tgtaaacgct tcacagacga tgacatctgc tccgccatgc
      721 ggaatttccc ttgcttcaat actgccaata aagttaatat caggacaggc ttttaaaagc
      781 gggaatgttt cttttacaag ggcatttccc ttttcttctt ctgctccgat atttacaata
      841 gcgactcttg ggttcttaat tcccacaacg tgttccatat aaatggaacc catctgcgca
      901 aactgtacca gatgagatgg tctggcatct acgttggcgc cacagtcgat cagcagtgac
      961 acgccttttt cagtagggat aagcggtgcc agcggaggac gttctacgcc cttgatcctg
     1021 cctacgatca cctgtccgcc tactaaaata gctccggaac ttccagcgga tacaaaagca
     1081 tctgcttctt tattctttac aagattcatg cctaccacaa tagatgagtc cttcttctta
     1141 cggatcgcat tcaccggcgg ttcttctgtg gcaatgactt ctgtggcatt caccacctgg
     1201 atctgctctt tgttgtaagt atatttggaa agctctttat ttactactgc ttcctgacct
     1261 actaaaagca cctggatccc tggttttgcg tttaccgctt ctacggctcc cgcaaccatc
     1321 tcaactggtg ccaggtcacc gcccatagca tctacagcta ctttaatcat aaaagactct
     1381 cctttcggtt tcgcaataca gttattaata tactagatgt cgtatcataa atcaatacgt
     1441 ttttatgtga atttcgcccc gcctgataaa ggcagcgatc atgttttctt ttcaccgcaa
     1501 aagaggaccc tgagattact cccaggatcc tctgatcttc gtttttctac agattattca
     1561 gctgtattaa cgatttcgcg cttattgtag cttccgcaag ccttgcatac tctgtgaggc
     1621 atcataaggg ctccgcactt gctgcactta accaggtttg gtgcgctcat cttccagttt
     1681 gctctacggc tgtctcttct agctttagaa gatttattct ttggacaaat agacatggtt
     1741 tacacctcct tcaactgttt aaaaatatcc tggataactg acattcttgg gtccagtgac
     1801 ctgttgtcgc agtcacaagt ccctcggttg agatttgtgc cacatctatt gcaaatcccc
     1861 ttgcagttat cactgcagag aactctcata ggcaggttca gggttaactc attaccaacc
     1921 agctggtcga catccagatt ataaccatta atatagaact gctcatccaa agcttctatg
     1981 cggtcttcct tcgtctgatt catatccagc tcctggtcga tctccagttc aaaaggaact
     2041 aaaacaggct ctaagcatct ggcacatggc atttcaaggc ttaaaacagc ctttcctgtc
     2101 acagtcagta cccgttctcc ctggttagtg ataaccagag tcaccggttc tttttccaca
     2161 actttataag aaccatcagg tcctttaaaa tgtgtcattt ccagtggaac tgtataggtt
     2221 ttggatttcc cttcaacagg gaacagctct gataaattaa tcagcatatc gtactccttg
     2281 atatgcacct atgctattat acataggcgc accgggtttg tcaacaaaat tttccaaaaa
     2341 aattacttaa ccagtcttaa agtctctctt gcgattgcca gctcttcgtt ggttgggatc
     2401 agcataactt ttaccttgga atctggagca gaaataattc ttctctctcc tcttacattg
     2461 ttagcttcgt catcgatctt aacgcctaag tagcctaagt attcgcaaac gttctttctg
     2521 gtaaccttat cgttttcacc aacaccagca gtgaatgcga tagcatctac accgttcata
     2581 gctgctgtgt aagcacctac gtacttagca acacggtata cgaatgcatc cagagctact
     2641 gctgccagat ggtttccttc ttcctttgcc ttctcgatat cacggaagtc gctggagatg
     2701 ccgccgctca tgcctaaaac accggacttc ttatttaaga tgttcagaac ttcgtttacg
     2761 tcttttcctt ccttgttgca gatgaactga acaactgcag gatcaacgtc accacttctg
     2821 gtacccatga tcaggccctc aagaggtgta agtcccatac tggtatctac gcacttgccg
     2881 ccgatggaag cagatacgct ggcaccattt cctaagtggc aaacgataac ttttgccttt
     2941 tctggatcta agttgccgaa cttgatcgct tcgccggata cgaacatatg gctggttccg
     3001 tggaagccat aacgacggat gctgtatttc tcatagtact catgaggaat tgcgtacata
     3061 taagccttct ctggcataga cataccaaat ccggtatccc atactgctac gtttggctta
     3121 ccaggcatag cctgttcaca agcctcgata cccattaagt tagctgggtt gtgcagaggt
     3181 cctaagtcga agcattcacg gataactctc ttaacatcct cgtttactac tacagactcg
     3241 ctgtaaacaa cgcctgcgtg cagtacgcgg tgtccgatcg cggagatctc atcggtgctt
     3301 gcgatcacgc cgtgatcttt gtcctgtaaa gcttccataa ccatgttgat agctacggtg
     3361 tggtttggca ttgggctttc aacaacatat ttgtccttgc ctgctggctg atgggtcagc
     3421 ttggaacctt cgataccgat tctctcgcaa aggcccttag caacaacgct ttcgttgtcc
     3481 atatcgatca gctggtactt taaggaagag cttccgcagt tgataactaa aatcttcata
     3541 ataggtaatt ctcctgtata tttattttac tatgaaaagt tccgggcgtc atatacgcat
     3601 aagcgcaggc acccggtaac tttttattta tcgcttctaa attagttgtt ctggcactgt
     3661 acagctgtga tagctacaac acctacgata tcgtcagcag agcatcctct ggacagatcg
     3721 tttactggct tagcaatacc ctggcacatt gggccgtaag cagctgcctt gccaagtctc
     3781 tgaaccagct tgtaaccgat gttaccagca tccaggtctg ggaataccag tacgttagca
     3841 tgtccagcaa ccttggaatc tggagccttg gaagcaccga tctcaggaac gattgcagcg
     3901 tctaactgca gttcgccgtc taatgctaat tctggagcca tttccttagc taacttggta
     3961 gcttctacta ctttgtctac gtcagcatgc tttgcgcttc ccttggaaga atgggacagc
     4021 atagcaacct ttggctcatg tcctactaac atcttgaagc tctcagcgga agaaacagcg
     4081 attgctgcta attcttctgg agttgggttc tggttcagac cgcagtcagc gaatacgaat
     4141 gtaccgttgt cgccaagatc gcaatccgga acttccatta agaagaatgc ggaaactaac
     4201 ttggttcctg gtttggtctt aataacctgt aagcaaggtc ttaaggtgtt tgctgtagaa
     4261 tggcaagcgc cggataccat accgtcagca tcgccgttct tgatcattaa acagccatag
     4321 taagcgtagt cgcctaagat ggtctctttt gccttctcag gagtcatgcc ttttgctttt
     4381 cttaactcaa cgaagagatc gatatattcc tgggtcttct cgtaggtctg tggattgatg
     4441 atggttgcgc cggaaacatc taagcccttg ctgtgctcgc cgatatcctc tggtgtaccg
     4501 ataatgatca ggttagcaag tccttctttt aaaattttct cagcagcttc ccatgttctg
     4561 cggtccatgg actctggaag aacgatagtt tttttgtctg cttttgcttt ttctttgata
     4621 atgtcgatta ctgccataat cttttttacc cctttcctta tgttgaagca tataacatta
     4681 ttatatacgt agcccccagc ggaaacaagc tgaatttaac gaaattttgc attttttttc
     4741 atacttttcg ttattttgga taagatgcag acatgtttcc caaaaatccc cggggtcaga
     4801 tcttttcgta atgatctaca ttgatcacaa agatagtagc tccgcctacc tctacagtca
     4861 taggcatggt agcatagttt accatggcag taccggaaat atacggcatg ttca
//
LOCUS       DBMDUA010000195         4914 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6494_length_4914_cov_1.776647, whole genome shotgun sequence.
ACCESSION   DBMDUA010000195 DBMDUA010000000
VERSION     DBMDUA010000195.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6494_length_4914_cov_1.776647"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            356..1300
                     /locus_tag="ACZPCL_11680"
     CDS             356..1300
                     /locus_tag="ACZPCL_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163162.1"
                     /translation="MRDEKSGKESKQVNFRIDPDSAEAFRLFCTTHGMNQAQGFDHLI
                     QVLELNNAKAATPGRAVEIENFERLLKEILSAYIGSIELSSNAEARVLERFQSDLKRK
                     DKTIDELREKVETIAGEKNNQDAALTALKEEQVRTKEQAQHAISQLESAKKATADQEE
                     INRILQEQNQQLQEKASRYDSLKKSDDANKEENTKLQLRIRELEQMLSHETALRTDLE
                     NTSKQASGRIRELESQQIECRIQISDLQRQLADKDSDIENRLKQAKMQADLEQERAVI
                     AKERELQDELRKMDRDNVKLRTELEQTKAALEQEMKKK"
     gene            1318..2361
                     /locus_tag="ACZPCL_11685"
     CDS             1318..2361
                     /locus_tag="ACZPCL_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009662768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1163163.1"
                     /translation="MEELQYHEQRDLKNKKKLQELLAALPSFCADFFRGIEPATSSMT
                     RLSYAYDISTFFDFLRKLKPELEKLSVENISLEQLCAVTIQDLERYLEYLKYRPDDPE
                     HKNVNKEQGIKRKFFSLKSFFGYFYRTGQIKTNPTLSMQVPKIREKEIIRLNQDEIKT
                     LLKEAESGSGLSQKQKQFHEKTKVRDCAILALMLGTGIRVSECVGLNLDDLDLEENGI
                     HIHRKGGKESTVYFSDEVAQYLKRYFEERTAQDLDLQQEPALFLSLRKQRIAVRSVEY
                     LVKKYAAIATPKKKITPHKLRSSYGSALYAKTNDIYLVADILGHNDVNITRKFYAAIE
                     ENRRKSVKNIDIL"
     gene            complement(2416..3297)
                     /locus_tag="ACZPCL_11690"
     CDS             complement(2416..3297)
                     /locus_tag="ACZPCL_11690"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR1163164.1"
                     /translation="MVDLKEFEQKAADLRVLGMKISIGQDTVYEKYWDEDCRRNIYSA
                     SKSFTSCAVGFAVQEGLIDLDERLMDAFKEDIPNNPDENLKKATVKDLLTMTLGQEKG
                     FLMGKQRPRMEEQSWVKASLAIPFVYEPGTHFVYNNVGPYLAGILVQRRAGCDLLSYL
                     TPRLLKPLGIRKTIWEVDPEGNTFGAGGLFLTVSELHKLGMFYLQDGCWNGKKLLSKE
                     WIHESTRKQVENNKNSYGYGYLFWGGRYNSFLANGKYGQYTMIFRDKNTVISLMAESR
                     DTDHLEEIIANEVYTKL"
     gene            complement(3310..4887)
                     /locus_tag="ACZPCL_11695"
     CDS             complement(3310..4887)
                     /locus_tag="ACZPCL_11695"
                     /inference="COORDINATES: protein motif:HMM:NF019157.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HIR1163165.1"
                     /translation="MKEKISLLKRVSIVDDARSILRDMVIPPELLKENTNGGRITSVC
                     EKSKEGGTAYLEVMGVIEPLDPLAPCIEWKILLPTQWNLRSVQIGGGANNGMIPSLEG
                     AMLMSDYCPIEHGYVVFGDDSGHQSADPMSADFAANEEALQNYIRHHLIKMNGIMHVV
                     VKAAYGIDAEKVYFAGGSAGGREALECACSYGKDYDGIFCADPVSNFVLLRMWGALLS
                     KAIYDSYDEHTHPFSDGFIDEKTVEAIRNDAIEMYDELDGVKDGIVSNIYAARMNRDV
                     FLRKIQEKYHLTDAQIQTIQVYEDGFKLDYSMPNGEKRYHGYCALEGGIMDLGPDPVP
                     REPLDTRYNVHHGDRSDGVFKYFITKDKNWKLIDHDYYKPDEKLYHMLMEASSQYDVS
                     MDFDEFVSHGGKLILFTSWNDMSISPWQIINQYQKLVKKYGQKKADSFMKFYCMPSAT
                     HCSGIRMDYLEWLDTWCSEEKYPEETLYGVIEKTGGEMPMATFPGWVKYKDGDPLKGT
                     SYEVSYEIPEGFFDNFA"
BASE COUNT         1499 a         1064 c          939 g         1412 t
ORIGIN      
        1 tacataaaat cttatttata caatcaaaaa tgccatataa tcagttaatt gtttaatata
       61 tcagtcttta taaacattgc gcaaaactaa ggttttacgc aatgttaaag ccgatttttc
      121 gaatatatgt tctgatttta acttattttt cttgaatttt cttaattgtg tataatgtac
      181 acctaattat ctgacattta tcctgtgata attacgctat aatcccctgt ttttatctat
      241 tatatcacgt tatacaaaga tatgtataac atatcactgt ataacaatgt tatactatca
      301 atttgacgaa atcacttctc ctgtgatata ctatttttta ggaggatttt aaaatatgag
      361 agacgaaaaa agcggaaaag aaagcaaaca ggtcaatttc cggatcgatc cagactcagc
      421 ggaagcattt cgcttgtttt gtaccaccca tgggatgaat caggcccagg gatttgacca
      481 tcttatacag gttttggaat taaataatgc taaggcggca acaccggggc gtgctgtgga
      541 gattgaaaat tttgagcggc tgttaaagga aatcctgtcg gcttatattg gcagtattga
      601 attaagcagc aatgcagagg cacgggtgtt ggagcgtttt cagtcggact taaaacgtaa
      661 agataaaacc attgatgagt tgagagaaaa agtcgagaca atagccggag aaaaaaataa
      721 ccaggatgct gctctcactg ccttaaaaga ggaacaggtc cggaccaaag aacaggccca
      781 gcatgccata agccagttag agtctgcaaa aaaagctact gcggatcagg aagagatcaa
      841 tcgtatcctt caggaacaaa atcagcagct tcaggagaag gcctccaggt atgactctct
      901 taaaaaatcc gatgatgcaa acaaagaaga aaatacaaag cttcagctgc ggatccggga
      961 attggaacaa atgctttccc acgaaactgc tctgcgaaca gatctggaaa atacttctaa
     1021 acaggcatct gggcggatcc gggagttaga aagccaacag attgaatgca ggatccagat
     1081 ttctgactta caacgacagc ttgcagataa agacagtgat atagaaaatc gattaaaaca
     1141 ggctaaaatg caggcggatt tggaacagga acgggcggtc attgcaaaag aacgggaatt
     1201 acaggatgaa cttcggaaaa tggatcgtga taatgtcaaa ttgcgcacgg aattagaaca
     1261 gacaaaggct gctttagaac aggaaatgaa aaagaaataa acaaggggtg atcggatatg
     1321 gaagaattac aataccatga acagcgtgat ctgaaaaata aaaagaaact gcaggaactg
     1381 cttgctgctc tcccatcatt ttgcgccgac ttttttcgag gaattgaacc tgccacatct
     1441 tccatgacaa gactctccta cgcctacgac atcagcacat tttttgattt tttaagaaaa
     1501 ttaaagccgg aacttgaaaa attgtctgta gaaaatattt ccctggaaca gctttgcgca
     1561 gttacgatcc aggatcttga gcgttatctt gaatatttaa aataccgtcc tgatgatcct
     1621 gaacataaga atgtaaacaa agaacagggg atcaagcgga aatttttctc tttaaagagc
     1681 tttttcggtt atttttatcg aaccggacag attaaaacca atcctaccct ttctatgcag
     1741 gttccgaaga tccgggaaaa ggagatcatc cgcttaaatc aggatgagat caagacactt
     1801 ttaaaagagg cagaaagcgg ttctgggctt tctcaaaagc agaagcagtt tcatgaaaaa
     1861 accaaagtac gggattgtgc tattctcgcc cttatgcttg gtacagggat ccgtgtatca
     1921 gaatgtgtcg ggttaaatct ggacgatctg gatctggaag aaaatggaat ccatatccac
     1981 agaaaaggcg gaaaagagtc aaccgtttac ttcagtgacg aggtggcgca atatttaaaa
     2041 cgctactttg aggagcgaac tgcacaagat ttggatctcc aacaggaacc tgcccttttt
     2101 ctgtctctga gaaaacagcg cattgctgta cgcagtgttg aatatcttgt aaaaaaatac
     2161 gccgctattg caacacctaa aaagaaaatc acaccccaca agttaagaag cagttatgga
     2221 tctgcacttt atgcaaaaac caatgatatt tatctggttg ctgatatttt aggacataac
     2281 gatgttaata ttacaagaaa attctatgca gccattgagg agaaccggag aaaaagcgtt
     2341 aaaaatattg atattctgta aaataacagg cggagaagat attgcatcct ttccgcccaa
     2401 aattatctaa gttctttata attttgtata tacttcattt gcaatgattt cttctaaatg
     2461 atctgtatcc ctgctttctg ccattaaact gatcactgta tttttatccc gaaagatcat
     2521 ggtgtattga ccatattttc cattggccag aaaactgtta taacggccgc cccagaataa
     2581 gtatccataa ccataagagt ttttattatt ttccacctgt tttctggtac tctcatggat
     2641 ccattcctta gaaagcagct tttttccatt ccaacagcca tcctgcaagt agaacattcc
     2701 cagtttgtga agctcggaaa cagtcagaaa cagtccgccc gcaccaaatg tatttccctc
     2761 agggtctacc tcccatatgg tttttcgtat tcccaaaggt tttaaaagcc gtggagtcag
     2821 atatgacaaa agatcgcatc ctgctcttcg ttgaaccagg atcccagcca gataagggcc
     2881 aacgttattg tatacaaaat gggttcccgg ctcatacaca aagggaatag caagggatgc
     2941 ttttacccag ctctgttctt ccattcttgg acgctgtttg cccataagaa agcctttttc
     3001 ctggcctaaa gtcatggtca gaaggtcttt cactgtggct ttctttaaat tttcatccgg
     3061 gttgtttgga atatcctcct taaaagcatc catcaaacgt tcatctaagt cgatcagacc
     3121 ttcctgaact gcaaatccca cagcacagga agtaaaactt ttactggcag aataaatatt
     3181 tctccggcag tcttcgtccc agtatttttc atataccgtg tcctgtccaa tagaaatttt
     3241 cattccaaga acccttaagt ctgctgcttt ctgttcaaat tctttcagat ctaccatata
     3301 ctttccttcc tatgcaaaat tatcgaaaaa cccttctggg atttcataag aaacctcata
     3361 gctggttccc tttaaaggat ctccgtcttt atatttcacc catcccggga atgttgccat
     3421 aggcatttca ccgccggtct tttcgattac tccgtagaga gtttcttctg gatatttttc
     3481 ttctgagcac caggtatcca gccattccag ataatccata cggatgccgc tgcaatgggt
     3541 ggcagaaggc atgcagtaaa atttcataaa actgtcggcc ttcttttgtc cgtatttctt
     3601 taccagtttc tggtactgat tgatgatctg ccaggggctg atagacatat cattccagct
     3661 ggtaaacagg atcagctttc cgccatgtga tacaaattca tcaaaatcca tacttacatc
     3721 atactggctg gaggcttcca tcagcatatg ataaagcttt tcatccggct tataataatc
     3781 gtgatcgatc aatttccagt ttttatcttt cgttataaaa tatttgaata cgccatctga
     3841 acggtctcca tggtgtacgt tatacctggt gtctaagggt tctcttggaa caggatcagg
     3901 accaagatcc atgatgccgc cctctaaagc acagtatcca tgataccttt tctcaccgtt
     3961 tggcatagaa taatccagtt taaaaccgtc ctcatacacc tggatcgtct ggatctgggc
     4021 gtctgtcaga tgatatttct cctggatttt cctgagaaat acgtcccggt tcatacgtgc
     4081 tgcatatata ttggacacga tcccatcttt tacaccatcc agttcatcat acatctcaat
     4141 agcatcatta cggattgctt ctactgtctt ttcatctata aagccatctg aaaacgggtg
     4201 tgtgtgttca tcgtaactat catatattgc cttggaaagc aaagcgcccc acatacgcag
     4261 aaggacaaaa ttggatacag gatctgcgca gaatatccca tcgtaatctt ttccatagga
     4321 gcaggcacac tccaaagcct ctcttccacc tgcagatcca ccggcaaaat aaactttctc
     4381 cgcgtcgatc ccataagctg cttttacaac cacatgcata atgccgttca ttttaatcag
     4441 atgatgccgg atataattct gaagagcctc ctcatttgct gcaaaatcag cagacatagg
     4501 atcagcagac tgatggccgg aatcatctcc aaatacgaca tatccatgtt cgatgggaca
     4561 ataatcagac atcagcatag caccttctaa agaaggaatc attccgttat tggctccgcc
     4621 gccgatctga acacttctta aattccactg ggtgggaagc aggatcttcc attcgataca
     4681 gggagccaaa ggatccagtg gttcgatcac ccccataact tccaaatatg cagttccacc
     4741 ctcttttgac ttttcacata cgctggttat ccttccacca tttgtattct cttttaacag
     4801 ttccggcggg atcaccatat cccttaaaat agaacgggca tcatcaacga tggatacccg
     4861 ttttaataag ctgatctttt ctttcatttt gtccactgcc ttcttcattg ttta
//
LOCUS       DBMDUA010000196         4888 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6524_length_4888_cov_2.273031, whole genome shotgun sequence.
ACCESSION   DBMDUA010000196 DBMDUA010000000
VERSION     DBMDUA010000196.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6524_length_4888_cov_2.273031"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            14..4612
                     /gene="essC"
                     /locus_tag="ACZPCL_11700"
     CDS             14..4612
                     /gene="essC"
                     /locus_tag="ACZPCL_11700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HIR1163166.1"
                     /translation="MALLMTVFFENGFSENYFPNVDNRNFPIAIRPYVSGLKEELVLN
                     AEVWDGQWKLRRSPQYSLILKGDPVESADLKDGTVIEGMVTKTDLYFSVKIDQVQREH
                     VNFEKYMLKEGSIFKLEIGSDDLCNIVYKNRFVTGSHAEITFESGAAYITDKNSVNGT
                     FLNGRLVREKTKLHYGDIIYVVGLKIVYLNNLLAINNPNNQCRIRELKAVEIPHFEDE
                     PSEETEVEDVYFLRTPRKLLNLDRETISIEKCPKKQQQKRQPLIFTIGPSFTMVIPIA
                     LAAAMTSDGFGAGSICMSLGAAGIGAIWAVVNSAYNKKEEKESEESRVSKYLEYMVRV
                     EEKLKNKTEYNRLVLAEQYPSSGELLEFTTSNTRRLWEKSSTHEDFLSLRLGAGDMPS
                     PNEIEDAKEDLGSEHDPLNDHIEELKEKFDILRQVSVAVSLYDHRLLGVVSGDEEKRD
                     QLMRILALQIAALHPYTDVRMCYVFPGRDLEKMEYTRWLPHTYTPDGKLRMIVCDSKA
                     MGDVMYYLSDVIRERLEAGENRKNKEEEEKVLPHYVVFISDISMIEGEPVSKYLLDPP
                     KNAGVSVIFSADAIDKLPSHCNTIVQWEKDYSGCYNTLSKFEEREGVAFDRVSLAEMD
                     VFSRQLSNFKVRENASNAAIPDMLTFLDMYKTSRVEDLDMYHKWLENRTYESMRSLIG
                     QKAGEQPVYLDIHEKYHGPHGLVAGTTGSGKSETLQTYILSLVLNYHPHEVAFILIDY
                     KGGGMAQSFIGLPHLAGVITNLGGNQTTRALLSINAEIKRRQRIFNEYKIKHIDAYIE
                     LYRNGEAEEPMPHLLIIADEFAELKKEQPEFVRALVSAARVGRSLGINLILATQKPSG
                     VVDDEIWSNTRFRICLRVADKQDSNEMLKRTDAAYITGTGRGFLQVGNDEIFDEFQSG
                     WSGAPYTPEIPFSDDSKAKAMIIGLTGKPEAVKKKKKKKGDNVKKFTQLDAMVQYAAK
                     LAEENHIKPLRQIWLPPLPKLLYLEDMKLTWDEKQMKLPIGLADDPQNQRQFPVYLDF
                     IRDGHLLICGSAGSGKTSLVQTILYGAALHYTAKQVNFYIADFSSRTMTAFAGLPHTG
                     CICMEGDDEKIQQMMGFAEEELDSRKKSFSQKGMGSYRDYRESYSDVPAIFLVIDNYP
                     AFSDSYEQYESTLIQLSREGASYGIYLILTCNNSGDIRSRILQNITKGIGLQLADRFE
                     YDSVIGMRSEILPDDRTTGRGLIKEGVPLEFQAALPVKASQGESMMLAIREKLKALTE
                     EGTQGARKLGVQLDEINADTFLEMIHLKGLAEENFVMGAEDGSLISTDLDKTLCFTVC
                     GAGKKGKTNFLKYTALLMKKKGAEVYVFDGCLRELEEFSRNNDLDGYMTDKEELYHFM
                     ENELLPQLGERNELVYEARQAKTSVKEALKNYKRMVLLINSASEFMEAVYSEDFDVSE
                     VLETVFEKGMNHRLHFFMALSPREYEELAGYRAIRQFGDWKQGIHLGGAFDEQGIFDY
                     EITPSERSRTYPAGAAFTGQEGRAVLFMTPFVKEGEHE"
     gene            4605..4874
                     /locus_tag="ACZPCL_11705"
     CDS             4605..4874
                     /locus_tag="ACZPCL_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163167.1"
                     /translation="MSKIHLEIEIPGNAKTYEFVLDDRMKIGTAKEQIKKQIAELEGH
                     DLFAGLDNIVFGSLELEGLIQDNETFREVGIKNGSRDTTDQFLFD"
BASE COUNT         1484 a          954 c         1221 g         1229 t
ORIGIN      
        1 aggagggaga tctatggcat tacttatgac cgttttcttt gaaaatggct tttcggaaaa
       61 ttatttcccc aatgtagata accgcaattt ccccattgcc atacgtccct atgtaagcgg
      121 tttaaaagaa gaactggtat taaatgcaga agtctgggac ggacaatgga aactgcgaag
      181 atccccacag tattccctga tcttaaaggg agatccggtg gaaagtgcgg atctaaaaga
      241 tggtaccgtg atcgagggaa tggtgaccaa aacagacctg tacttttctg ttaagatcga
      301 tcaggtccag agggaacatg taaattttga aaaatatatg ctgaaagagg gcagcatctt
      361 caaactggag atcggcagtg atgatctttg taatattgta tacaaaaacc gctttgtaac
      421 aggaagccat gcagagatca cttttgagtc aggagctgct tacataaccg ataaaaacag
      481 cgtaaatggc acattcttaa acggcaggct ggtaagggag aaaacaaagc tgcattacgg
      541 cgatattatt tacgtagtag gtcttaagat cgtatatctg aataatcttc tggccatcaa
      601 taatcccaat aaccagtgca ggatccggga gctgaaagca gtagagatcc cacattttga
      661 agatgaacca tcagaagaga cagaggtgga agatgtatat ttcctgcgca ctccacgaaa
      721 gcttttaaat ttggaccggg aaaccatttc cattgaaaaa tgccctaaaa aacagcagca
      781 gaagcgacag cctttgatct ttaccatagg accttctttt accatggtca tccccatcgc
      841 tttagcagca gccatgacca gcgatggctt tggtgcagga agtatctgca tgtccttagg
      901 tgcagccggg atcggggcta tatgggcggt agttaattct gcttacaaca aaaaagagga
      961 aaaagagtca gaagaaagcc gggtcagcaa atatttagag tacatggtcc gtgtagaaga
     1021 aaaattgaaa aataaaacgg agtataaccg tctcgtactg gcagagcaat acccttcttc
     1081 tggggaactt ctggaattta ccacttccaa tacccgcagg ctctgggaaa aaagcagtac
     1141 ccatgaagat tttctttctc tccgtttagg agcaggtgat atgccttctc ctaatgagat
     1201 tgaggatgca aaagaagatc tgggcagtga acatgatcct ttaaatgacc atatagaaga
     1261 actgaaggag aaatttgata tacttcgcca ggtttcggtg gcagtttccc tttatgatca
     1321 ccgcctctta ggtgtagtaa gcggggatga ggaaaaacgg gatcagctga tgcgcatttt
     1381 agccttgcag atcgctgccc tccacccata tacggatgta cgcatgtgct atgtatttcc
     1441 aggcagagac cttgaaaaaa tggaatatac ccgctggctt ccccacactt atacaccgga
     1501 cggaaagctt cgcatgattg tctgcgactc caaagccatg ggcgatgtga tgtattacct
     1561 gtctgatgtg atcagagaac ggttagaagc aggggaaaac aggaaaaaca aggaagaaga
     1621 ggaaaaagtc cttcctcatt atgtagtctt tatcagtgat atttccatga tcgaagggga
     1681 acctgtttcc aagtatcttt tagatccgcc gaagaatgca ggggttagtg tgatcttcag
     1741 tgcagatgcc attgataagc ttccgtccca ttgcaatacc attgtacagt gggaaaagga
     1801 ttacagtggt tgttacaata cgttatctaa atttgaagaa cgggaaggag tagcctttga
     1861 ccgtgtcagc ctggccgaaa tggatgtatt ttccaggcag ctttccaact ttaaagtacg
     1921 ggaaaatgca tccaatgcag cgatcccgga tatgcttacc ttcttagata tgtataagac
     1981 ttccagggtg gaagatctgg atatgtacca taagtggctg gaaaaccgca catatgaaag
     2041 tatgcgttcc ctcatcggtc agaaggcagg ggaacagccg gtttatctgg atatccatga
     2101 aaaataccat ggtccccacg gtctggtggc tggtactaca ggaagcggaa agtcagaaac
     2161 actgcagacc tatattttaa gccttgtatt aaattatcat cctcatgagg tggcatttat
     2221 cctcatcgat tacaaaggcg gtggcatggc ccaaagcttt atcggacttc ctcatctggc
     2281 aggtgtgatc accaacctgg gaggaaacca gactaccagg gcccttcttt ccattaacgc
     2341 ggagatcaag cgtagacagc gcattttcaa cgaatataag atcaagcaca ttgacgccta
     2401 tattgaactt tacagaaatg gggaagcaga agaaccaatg cctcatcttc tgatcattgc
     2461 cgatgagttt gctgagctta aaaaagaaca gccggaattc gtccgggcgc tggtttccgc
     2521 agcccgtgta ggtcgttctt taggtattaa tctgatcctt gcaacccaga agccaagcgg
     2581 cgtagtagat gatgagatat ggagcaatac aagatttcgt atctgtcttc gtgtggcaga
     2641 caaacaggac tccaatgaaa tgttaaagcg cacggatgca gcttacatca ccggaaccgg
     2701 acggggcttt ttacaggttg gaaatgatga gatctttgat gaatttcagt ccggctggtc
     2761 cggagcacct tacacgccgg aaattccatt tagtgatgac agcaaggcaa aagccatgat
     2821 catagggctg acaggaaagc cggaggcagt gaagaagaaa aagaagaaaa aaggggataa
     2881 tgtgaagaaa ttcacccagt tagacgctat ggtccagtat gcggcaaagc tggcggagga
     2941 aaatcatatt aaacctctca gacagatctg gctgccgccg cttccaaaac ttctttatct
     3001 ggaggatatg aagcttacct gggatgaaaa acagatgaaa cttcctattg gtctggcaga
     3061 tgatccgcag aaccagaggc agtttccggt ttatcttgat tttataagag atggtcattt
     3121 actgatctgc ggttcagctg gttcaggaaa gacatctctg gtacagacca ttctttacgg
     3181 tgcggccctt cattatacgg caaaacaggt gaacttttat atagcagatt tttccagccg
     3241 taccatgact gcctttgcag gacttcccca tacaggctgt atctgtatgg agggggatga
     3301 tgagaagatc cagcagatga tgggttttgc agaggaagaa ttagacagtc gaaagaagtc
     3361 attttcacaa aaaggaatgg gctcttacag agactatcgt gaaagttaca gcgatgtgcc
     3421 ggcgatcttt ttagtcattg acaattatcc cgccttttca gacagctatg agcagtatga
     3481 gagtactttg atccagcttt ccagagaagg tgccagctat ggtatttacc tgatccttac
     3541 ctgcaacaat tccggtgata tccgaagccg tattttacag aatatcacaa agggtattgg
     3601 acttcagtta gcagaccggt ttgagtatga cagtgtgatc gggatgcgct ccgagatcct
     3661 tccggatgac cggactacag gcagaggtct tataaaagaa ggcgttcctt tggaattcca
     3721 ggcagctctt cctgtaaaag cttcccaggg agaaagcatg atgctggcga tccgggaaaa
     3781 attaaaggct ttaacagaag aaggtaccca gggagcgaga aaactgggag tgcagttaga
     3841 tgagatcaat gcagatacat ttttggagat gatccatctt aaagggctgg cagaagagaa
     3901 ctttgtaatg ggagcagagg atggatccct tatttccaca gatttagata agaccctttg
     3961 ttttaccgtc tgtggtgcag gaaaaaaagg aaagaccaat tttctaaaat atacggccct
     4021 gcttatgaag aaaaagggcg cagaagttta tgtctttgac gggtgtttaa gagaattgga
     4081 agaattttcc agaaacaatg atctggatgg ctacatgaca gataaagaag aactttatca
     4141 ttttatggaa aatgaacttt tgccccagtt aggagagcgc aatgaacttg tgtatgaagc
     4201 gagacaggcg aaaacttcgg taaaagaggc tttaaagaac tataagcgca tggtccttct
     4261 gatcaacagc gcatcggaat ttatggaagc agtttacagt gaagactttg atgtaagtga
     4321 agtccttgaa actgtatttg aaaaaggaat gaatcacaga ctgcatttct ttatggcact
     4381 gtctccaaga gagtatgaag aactggccgg ttacagggcg attcgtcagt ttggagactg
     4441 gaagcaggga atccaccttg ggggagcttt tgatgagcag ggtatttttg attatgagat
     4501 cacaccttct gagaggagca ggacttatcc ggctggagcc gcatttacgg ggcaggaagg
     4561 aagagcagtc ctctttatga caccttttgt aaaggaggga gaacatgagt aagatccatt
     4621 tggagatcga gataccggga aatgccaaaa cctatgaatt tgtgttagac gaccggatga
     4681 aaataggaac tgccaaggag cagatcaaaa agcagatcgc agagttagag gggcacgacc
     4741 tgtttgcagg actggataat attgttttcg gatctttgga gttagaaggt ctgatccagg
     4801 ataacgaaac cttccgggaa gtagggatca aaaacggcag ccgggatact acggaccaat
     4861 ttttgttcga ttaattcagg aaacaatt
//
LOCUS       DBMDUA010000197         4837 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6597_length_4837_cov_2.677998, whole genome shotgun sequence.
ACCESSION   DBMDUA010000197 DBMDUA010000000
VERSION     DBMDUA010000197.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6597_length_4837_cov_2.677998"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            78..1007
                     /locus_tag="ACZPCL_11710"
     CDS             78..1007
                     /locus_tag="ACZPCL_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009533426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1163168.1"
                     /translation="MFQGMHYVYEVYKEMSFSKAARNLFISQPSLSAAVKKAEKQIGF
                     PIFDRSSNHIQLTELGKEYIRSIEIIMDVETGFQNYINDMKELKSGTIAIGGTNLFAS
                     YVLPPLLSRFTEQYPQVQVDLVEATTSQLTDRLFSGSLDLLIDNKNMDPAVYQKSFFC
                     EEHLLLAVPIRLAVNEKMKNYALTASDIRENRHLNSRIPPVPLENFQNESFLLLKSGN
                     DTRSRADRICHNAHVLPKIKLELDQQITAYNLSRYGMGISFCSDTLVRHVPDDEGLVY
                     YKLDHKDALREVNFYYKRGRYMPRVVSTFLEMI"
     gene            complement(1195..1971)
                     /gene="trpA"
                     /locus_tag="ACZPCL_11715"
     CDS             complement(1195..1971)
                     /gene="trpA"
                     /locus_tag="ACZPCL_11715"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR1163169.1"
                     /translation="MTKIRDAFKDGKAFIPFLTCGDPDLETTEKLVCAMAEAGADLIE
                     LGIPFSDPTAEGPVIQEANVRALSAGTTTDKIFDMVVRIRKTVKISLVFMTYANVVFS
                     YGSERFISKAAEIGINGLILPDVPYEEKEEFEPICKKYGLDLISMIAPTSDHRIAMVA
                     KEATGFIYVVSSLGVTGVRSEIKTDIGALVEQIRAVTDVPCAVGFGISEPEQAKKMAG
                     LSDGAIVGSAVVKRIAKYGKDCVPYVAEYVKEMKQAVREA"
     gene            complement(1964..3154)
                     /gene="trpB"
                     /locus_tag="ACZPCL_11720"
     CDS             complement(1964..3154)
                     /gene="trpB"
                     /locus_tag="ACZPCL_11720"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR1163170.1"
                     /translation="MSNAKGRFGIHGGQYIPETLMNAVIELEEAYNHYKDDPQFQAEL
                     TELLNEYAGRPSRLYYAEKMTKDLGGAKIYLKREDLNHTGAHKINNVLGQALLAKKMG
                     KTRLIAETGAGQHGVATATAAALMGMECVVYMGKEDTERQALNVYRMRLLGATVIPVT
                     TGTGTLKDACSACFREWTNRISDTHYCLGSVMGPHPFPTIVRDFQAVISKEIKEQLME
                     KEGKLPDAVLACVGGGSNAIGAFYNFINDPSVKLIGCEAAGRGADTPETAATIATGRL
                     GIFHGMKSYFCQDEYGQIAPVYSISAGLDYPGIGPEHAMLHDTGRATYVPVTDDEAVD
                     AFEYLSRTEGIIPAIESAHAVAYAKKLAPTMGKDQIIVINISGRGDKDCAAIARYRGE
                     NIYD"
     gene            complement(3634..>4837)
                     /locus_tag="ACZPCL_11725"
     CDS             complement(3634..>4837)
                     /locus_tag="ACZPCL_11725"
                     /inference="COORDINATES: protein motif:HMM:NF013696.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1163171.1"
                     /translation="EEIGIQVTKYSVKPAPVLVGRFGNDPEKKTVLIYAHYDVKPAGE
                     RKLWHSDPFVPTVRDGKVYARGSADNKSPLMAHLKALEYFIRRKMEVPVNVIFVFEGD
                     EECGSRGLEEFLAEHRTELAADLVFFSDGPKDPSGLPIIALGAKGDLSIHITVETMNR
                     NVHARYAPVLPSAAWQLVEILGKCRSKDKILIPGFEEGILPFTKKEIEIMDKLPKSED
                     SLNTIYEAKSSNYGKDFYPRLLGQPTFNICWIKTGANGVVPAKAEALIDCRLVPDQDP
                     EKVFQCIKRHVEMMGYDNVKIENDGYIPSSKTDVNTPYLPAVEEICRDIYGEYVIYPC
                     RPSSAPDYLWTNVLKLPAIQVRWSDADSDNHAPNEHLSIKEYMDGIALTVCALQAVSE
                     ISEINEKD"
BASE COUNT         1258 a         1211 c          936 g         1432 t
ORIGIN      
        1 ttataatttc atacttctta tagataaatg tctataatac attatctgta tgaataccaa
       61 ggacaaggag gactcccatg ttccagggaa tgcactatgt ttatgaagtt tacaaagaaa
      121 tgagtttttc caaagctgca cgaaatcttt ttatcagcca gccttcctta agtgctgcag
      181 ttaaaaaagc agaaaaacag atcggctttc ctatttttga ccgcagttcc aatcatatcc
      241 agctgacaga actaggaaaa gaatacatac gttccattga gatcatcatg gatgtggaaa
      301 ctggatttca gaattatatt aatgatatga aagaattaaa aagcggtacc attgccattg
      361 gcggcactaa tctgtttgcc tcttatgtgc tcccgcctct tttatcccgc tttacagaac
      421 aatatcccca ggtccaggtc gatctggtgg aagctaccac ttcccagctg acagaccgcc
      481 ttttctccgg ctccttagat ctgctcattg acaataaaaa catggatccg gctgtttacc
      541 agaaaagttt cttctgtgaa gaacacctgc tgttggctgt gcccatccgc ctggctgtaa
      601 atgaaaaaat gaaaaattat gcccttacag cttccgatat aagagaaaac cggcatctaa
      661 actcccggat cccgccggtt cccctggaaa attttcaaaa tgaatcgttt ttacttttaa
      721 aatccggaaa tgatacccgc tcccgggcag accgcatctg ccataatgcc catgttctcc
      781 ccaaaataaa actggagctg gaccagcaga tcactgccta caatctgtcc cggtatggaa
      841 tggggatctc tttctgttct gataccctgg tgcgccatgt tcctgacgat gaaggcctgg
      901 tatactacaa gctggatcat aaagatgctc tccgggaagt gaatttttat tataaacggg
      961 gccgctatat gccacgggta gtcagtacat ttctggaaat gatctaaaag atgattgtaa
     1021 taaataatct gtaaaacgat ctgcgagatg taccgatcaa tgattgtttc ttatctatca
     1081 tatctataac agttataacc atttgtctat agttcagtga cagatcacag tacttggcac
     1141 aaaaaaaggc tgctgcaaaa ggatttctcc ctctgcagca gcctttgggg cgccttatgc
     1201 ttcccttaca gcctgtttca tttcctttac atattccgct acataaggaa cgcagtcttt
     1261 tccatatttt gcgatccgtt ttacaacggc acttcctaca atggctccgt ctgacagccc
     1321 agccatcttc tttgcctgct ccggctcaga gataccaaaa cctactgcgc acggaacatc
     1381 ggtgaccgca cggatctgct ctactaaagc accgatatct gtcttgatct cgcttcttac
     1441 accggtaaca cctaaggaag atactacata gataaatcct gtcgcttctt ttgctaccat
     1501 tgcaattctg tggtcagaag ttggtgcgat catggagatc aggtccagac catatttctt
     1561 gcagatcggt tcaaattctt ctttttcctc atatggcaca tccggaagga tcagaccgtt
     1621 gatgccgatc tctgcagcct tggagataaa acgctcagag ccataggaaa atactacatt
     1681 tgcataggtc ataaacacaa gcgagatctt tactgtcttt ctgatacgga caaccatatc
     1741 aaagatctta tctgtggtag ttcctgcaga cagtgctctt acatttgctt cctggatcac
     1801 aggtccttct gctgtcggat cagaaaacgg gatccccagt tcgatcaggt ctgcacctgc
     1861 ctctgccata gcgcatacca gtttttcagt agtctccaga tccgggtctc cacaggttaa
     1921 aaacggaata aatgccttgc catctttaaa tgcatctctt atcttagtca taaatattct
     1981 cccctctgta gcgtgcgatc gctgcgcagt ctttgtctcc tcttccggaa atgttgataa
     2041 cgatgatctg gtcttttccc atagtcggcg ccagcttctt tgcataggca actgcatggg
     2101 cagactcaat ggcaggaatg atgccttcgg ttcttgacag gtattcaaaa gcatctactg
     2161 cttcatcatc ggttacaggt acataggttg cacggccggt atcatgtaac atagcatgtt
     2221 ccggtccgat gcccggataa tcaagtcctg cggaaataga atatacagga gcaatctgtc
     2281 cgtattcatc ctggcagaaa taagatttca tgccatggaa aattcccaga cggccggtag
     2341 caatggtcgc tgctgtttcc ggtgtatccg cgccccgtcc tgctgcctcg caaccgatca
     2401 gcttaacaga cggatcgttg ataaagttat aaaatgcgcc aatagcattg gaaccgccgc
     2461 ctacgcaggc aagcactgca tccggaagct ttccttcttt ttccatcagc tgttccttga
     2521 tctccttgga gataactgcc tggaagtctc ttacgatagt cgggaacgga tgtggtccca
     2581 taacagaacc aaggcagtaa tgggtatcgc tgatgcggtt ggtccactct ctgaagcatg
     2641 cagagcaggc atcctttaag gttccggtac ctgtagttac agggataacg gttgctccta
     2701 gcagtctcat acggtatacg tttaaagcct gacgctcggt gtcttcctta cccatataaa
     2761 ccacacattc cattcccata agagctgctg ctgtagcggt tgcaacaccg tgctggcctg
     2821 cgccggtctc agcgatgagg cgggtcttgc ccattttctt ggctaacagc gcctgtccta
     2881 aaacattgtt gatcttgtga gcgccagtgt ggtttaagtc ctctctcttt aagtagatct
     2941 tggctccgcc caggtctttg gtcatctttt ctgcataata cagacggctt ggacgacctg
     3001 catactcatt taacaattca gtcagctccg cctgaaactg aggatcatct ttgtaatgat
     3061 tgtaagcctc ttccagttcg atcactgcgt tcatcagtgt ttccggaata tactggccac
     3121 catggattcc gaaacgtcct tttgcatttg acatattttt ttcctcctct tgcctttcat
     3181 gtaccatttg tacacttatc tgtgtctgtt gtacatttac ctgtgttttt tccttctttt
     3241 ccttcatctg acgaggcagt tgtcaatgtt tcgtgcaaac acggactttg accggctgct
     3301 cacgcaaaaa agtccctgat gatacaaata tcatcaggga cggaataaac cgcggtgcca
     3361 cctctgtttc acataagctg tgcgctctgc gtagtacaat catactaccg gctgttaacg
     3421 tcagccccac gtcgcagaat actcgaagtc accttctttg actgcgccct caacggtcca
     3481 tttgacaatc tgggatctat cggacttcca ccaacgccga ctctctgtga ggccataact
     3541 gccgttatct ccgtttcaac ggttttctaa tgaagttgtt tggagtatag cactgtgacg
     3601 tggtgatgtc aagagttttt attcggattt tcttcagtcc ttttcgttaa tttctgaaat
     3661 ttcagaaact gcctgcaacg cacacactgt cagcgcaatt ccatccatat actctttaat
     3721 ggataaatgt tcatttggcg catgattatc tgaatcagca tcactccatc gcacctgaat
     3781 agccggaagc tttaaaacat tagtccacaa ataatcgggg gctgatgatg gtctgcatgg
     3841 gtagatcaca tattcaccat aaatatcacg acaaatttct tcaacagccg gcaaatacgg
     3901 cgtattcaca tctgtttttg aagaagggat ataaccatca ttttcaattt tcacattatc
     3961 ataccccatc atctcaacat gcctcttgat acattgaaat accttctcag gatcctgatc
     4021 tggtactaaa cggcaatcga tcaatgcttc cgcttttgca ggaactactc cattagctcc
     4081 tgttttaatc cagcaaatat taaatgtagg ctgtcctaaa agtctcggat aaaagtcctt
     4141 gccataatta gacgactttg cctcatagat tgtattcaaa ctgtcctctg acttcggcag
     4201 tttatccatg atctcaattt ctttttttgt aaaaggtaat attccttcct caaaaccagg
     4261 tatcagaatc ttatcttttg atctgcactt tcccaatatc tccaccaact gccacgcagc
     4321 tgatggcagt accggagcat aacgggcatg aacattccta ttcatcgttt caactgtaat
     4381 atgaatagac aaatctccct ttgcccccag agcaatgata ggaagtccac taggatcctt
     4441 tggaccatcg ctgaaaaaca ccagatctgc tgccaattcc gtcctgtgct ctgccagaaa
     4501 ttcctccaat cctctactgc cgcattcctc atccccttca aatacaaaaa taacatttac
     4561 gggaacttcc atttttcttc tgataaagta ttccaaagct tttaaatgag ccattaaagg
     4621 tgatttatta tcagcacttc ctcttgcata aacttttcca tcacgtactg taggaacaaa
     4681 cggatctgaa tgccacaatt ttcgttctcc agccggtttc acatcgtaat gggcatagat
     4741 cagaactgtc tttttttccg gatcattgcc aaaccgccct accaggactg gagcgggttt
     4801 tacagaatac tttgttactt gaattccaat ttcttcc
//
LOCUS       DBMDUA010000198         4783 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6685_length_4783_cov_4.756514, whole genome shotgun sequence.
ACCESSION   DBMDUA010000198 DBMDUA010000000
VERSION     DBMDUA010000198.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6685_length_4783_cov_4.756514"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(641..2515)
                     /locus_tag="ACZPCL_11730"
     CDS             complement(641..2515)
                     /locus_tag="ACZPCL_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1163172.1"
                     /translation="MTKKQKDMLKRILVTAVLYVALVATDHMKMVPAVFTGWKMLFLY
                     LIPYFVIGWDILYKAVRNIKNGQVFDENFLMAVATIGAFGVNEYSEAVAVMLFYQVGE
                     LFQSYAVNRSRQSITDLMDICPEYANIEEDGQLKQVDPDDVEVGDIIVIKPGEKIPLD
                     GKVVFGESMVDTSALTGESVPRRVKEGDDLVSGCVNGNGLLRAEVTKEFDDSTVAKIL
                     ELVENASSKKAHVENFITRFARYYTPAVVIGAVVLAVLPPLFLGQSWAEWVRRACTFL
                     VISCPCALVISVPMSFFSGIGAASKKGVLVKGSNYLETMAKLHTVVFDKTGTLTKGEF
                     KVAEIHSVDGDEKKQSMVLELAALAESYSDHPISRSIRDAWGKKLDQSRVSDAEEISG
                     HGVKVKIDGKTVLAGNGKLMDKEGISYTECASVGTVVYVEEEGKFLGSIVIADGIKDG
                     VKDAIRSLKRAGVSKTVMLTGDKRNVAEAVAKEIGLDEVHAELLPGDKVDRVEALLKA
                     LPEGRKLAFVGDGINDAPVLSRADVGIAMGSMGSDAAIEAADVVLMDDDPRKIADIVR
                     ISRKTMGIVMQNIVFALGVKFVVLAMGAFGVANMWEAVFADVGVSVIAILNAMRALRA
                     "
     gene            complement(2538..2756)
                     /locus_tag="ACZPCL_11735"
     CDS             complement(2538..2756)
                     /locus_tag="ACZPCL_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIR1163173.1"
                     /translation="MKKKFKLMDLDCANCAAKMEDAIKKIDGVNDATVSFMMQKLTLD
                     ADDDRFDEILKEAAEICKKVEPDCKIVM"
     gene            complement(2830..3189)
                     /locus_tag="ACZPCL_11740"
     CDS             complement(2830..3189)
                     /locus_tag="ACZPCL_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR1163174.1"
                     /translation="MALNDVEHCEACQIHEDVVKAVTDHMPDEDQLYDLAEVFKVFGD
                     STRIKILYVLFEAELCVCDIAQLLNMTQSAISHQLRILKQNRLVKNRRDGKSVFYSLA
                     DSHVKTIISQGMEHIQE"
     gene            complement(3397..4572)
                     /locus_tag="ACZPCL_11745"
     CDS             complement(3397..4572)
                     /locus_tag="ACZPCL_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR1163175.1"
                     /translation="MTTKNELFEKIEAQKEKLWEMADFIFDHPEYRGKEYQACDLLTK
                     YLTDNGFSVEKSVGGYETSFRAEWKHGEGGPVIGILCEYDALEGLGHGCGHHMQGPSC
                     LGAAVALKECAGEEPFTLVVYGTPAEETLGSKCDMIKNGCFHELDASFMMHGSPTTCT
                     DVKCLADQSFRVTFHGKRAHAALAPEQGRSAFDALLVAFNGIEFLREHVPDDVRMHYT
                     VAELPGPANVVPVKSVGKFSLRSFSKEELKGVVSRFKDIIKGAALIAGVDYELEQTSD
                     FYNKIPVLKLNELLMENAKLAGAPRLAPPREKTGSTDFGNVMYEIPGSCIRVAFVPEG
                     TSSHSQEFVDAGKTQAARDCILYGAKSIAGASMDLITKPELMDEVKAEFAENKKKFK"
     gene            complement(4591..>4783)
                     /locus_tag="ACZPCL_11750"
     CDS             complement(4591..>4783)
                     /locus_tag="ACZPCL_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HIR1163176.1"
                     /translation="AYNFGDGFCNYILPHSTALMGIISAVNIPYDRWMKFMWKLFLIW
                     AVTACIMTAIAQGIHLGPM"
BASE COUNT         1321 a         1328 c          820 g         1314 t
ORIGIN      
        1 tgcactctcg caattctaaa aacattcgta atccgctcat ttttcttcaa aaaatggctt
       61 cttactcatg tttttggcgg gtcccaagtt caaaaatcct catgcaagca agcttgcatc
      121 ggatttatga ccttttgacc ctggtatcaa aatattaggt tttattacct ttctactcta
      181 tatacagcca ggtcttttaa aatgttgcag aatttagaaa tttccctgct gtatgcctac
      241 cagtgcggaa aacggcgact ggaagccaaa gagaccagga tgacagaaca cctggccaaa
      301 ccattaaaac cagtgttttc accccgccat aagcggggtg gaagcacgac accggtcggc
      361 acactataaa agctcctgta aagaacacta actattccct acaggagctt tattacgcac
      421 ttcgcgcaat ttaaaatttt atcatggacc acacaggccg tcatttcact tatcaattgt
      481 ccacatcttg agtgcagctg tccgaatgca gcggacaaag tccgatgcag gcgaacaggt
      541 gcaaatcgaa agccagtgga caatgataag tgaaatgtaa ggccgtttct tgcacgattc
      601 acaacctcac agctgccagt ttccagcctg tcacgactct ttaggcccgc aacgccctca
      661 tagcattcag gatcgcaata accgaaactc caacatccgc aaaaaccgct tcccacatat
      721 tcgccacacc aaaagcgccc atagccaata ctacaaactt aacgcccagt gcaaacacaa
      781 tattctgcat aacgattccc atggtctttc tggagatcct tacaatatcc gcaatctttc
      841 tcggatcatc atccatgagc accacatccg cagcctcaat tgccgcatcc gatcccatac
      901 ttcccatagc aattcccaca tccgctctgg aaagcaccgg cgcatcatta ataccatcac
      961 caacaaaagc cagctttctg ccttccggaa gtgccttcaa cagcgcctcc acccgatcca
     1021 ccttgtcacc gggaagcagt tccgcatgaa cctcatccag tccaatctcc tttgccacag
     1081 cctcagccac attccgctta tcgccggtca gcatcacggt cttagaaaca ccggcacgct
     1141 tcaggcttcg gatcgcgtcc tttacgccat ccttaatacc atctgcaatt acaatagatc
     1201 caaggaactt tccttcctct tccacataca caaccgtacc aacagaagca cactctgtat
     1261 aagaaattcc ttccttatcc atcagctttc cattaccggc aagcactgtc ttcccgtcaa
     1321 tcttaacctt cacgccatga ccagaaattt cctcagcatc actcactctg ctctgatcca
     1381 gtttcttacc ccaggcatcc ctgatagacc gtgagattgg atgatccgaa taactttcag
     1441 ccagcgcagc cagttccaaa accatactct gctttttctc atctccgtca acactgtgga
     1501 tctcagctac cttaaactcg cccttagtca gcgtaccagt cttatcaaat accaccgtat
     1561 gcagctttgc catcgtctcc aggtagttac ttcccttcac cagaacgccc ttcttagaag
     1621 ccgcaccgat accagagaag aaactcatag gaaccgaaat aaccagcgca caagggcagg
     1681 agataaccag gaaagtacat gccctgcgta cccattcagc ccatgactgg cctaaaaaca
     1741 gaggcggaag tacagccaga acaactgcac cgatcacaac agccggagta taataccttg
     1801 caaaccgggt aataaaattc tccacatgag cctttttact gctggcattt tccaccagtt
     1861 ccaggatctt tgctaccgtg gagtcatcaa actctttggt aacttccgcc ctcaaaagcc
     1921 catttccatt gacacagccg cttaccagat cgtccccttc cttcactctt ctcggaacag
     1981 actcaccagt cagtgcagaa gtatccacca tagactcgcc aaaaacgacc ttgccgtcca
     2041 acgggatctt ctcacctggc ttgatcacga tgatatcgcc aacctccacg tcgtccggat
     2101 ccacctgctt taactgccca tcttcctcaa tattggcata ttccgggcag atatccatca
     2161 gatccgtaat agactggcgg gagcggttta ccgcataact ctggaacagt tcaccaacct
     2221 gataaaacag cataacagct actgcctcag aatactcatt cactccaaaa gctccaatag
     2281 tcgcaactgc catcaggaag ttctcatcaa aaacctgacc atttttaata ttccttactg
     2341 ccttataaag gatatcccag ccgatcacaa aataagggat gagatacaaa aatagcatct
     2401 tccagcctgt aaatactgcc gggaccattt tcatatgatc tgttgccacc agtgccacat
     2461 acagcactgc cgtcaccaga atacgcttca acatgtcttt ctgtttctta gtcatggcca
     2521 tctccttata tagcagatta cataacgatc ttgcagtctg gttctacttt cttacagatc
     2581 tctgctgctt ctttcaggat ctcatcaaaa cggtcatcat ctgcatccag agtcagtttc
     2641 tgcatcataa agctgactgt tgcatcattt acgccgtcaa tttttttaat agcgtcctcc
     2701 atctttgcag cacagttagc gcagtccaga tccatcagtt taaatttctt cttcatagtt
     2761 ttattcctcc tgatataaaa tattttttaa taaaacagct ctttcattta ctatatccat
     2821 atcccttcat tactcctgga tatgttccat tccctgactg atgatcgtct tcacatgact
     2881 atctgcaagg gaataaaaaa cggattttcc gtccctgcgg ttcttcacca ggcggttctg
     2941 ctttaagatt ctcagctgat gagagatcgc tgactgagtc atattaagca actgggcaat
     3001 atcacagaca cacagttctg cctcaaacag cacataaagg atctttatcc tggtagaatc
     3061 tccaaatacc ttaaaaacct ctgccagatc atacaactgg tcttcatccg gcatatggtc
     3121 tgtcactgct tttaccacgt cttcgtggat ctgacatgct tcgcagtgtt ccacatcgtt
     3181 taatgccata ggcggtgctc ctttcctttg tattcacata aatatttcgt atgaataatt
     3241 gttcatatgt ttatatttgc attataatac aacccatata aatgtcaata tgttttctga
     3301 aattttaacc ctggtatcat aaaatcccgg taagacattc tgcttttctt agcagccgtc
     3361 ttaccgggat caatatgatg ttcttactta ttctttttat ttgaactttt tcttgttttc
     3421 tgcaaactca gcctttactt catccatcag ctccggcttt gtgatcagat ccatagaagc
     3481 tccggcaatg gacttggcgc catagaggat gcagtctctt gcagcctgtg tctttcctgc
     3541 atctacaaat tcctgagagt gggaagatgt accttccggt acgaaagcta cacggataca
     3601 ggaacctgga atctcataca taacgtttcc aaaatcagtg gaaccagtct tttctcttgg
     3661 tggtgccagt cttggagcac ctgccagttt ggcattttcc ataagaagct catttaactt
     3721 aagaaccggg atcttattgt agaaatcgct ggtctgctct aattcataat caacacctgc
     3781 gatcagtgct gcacccttga taatgtcctt aaaacggctt acaacaccct ttaactcttc
     3841 cttggagaaa gaacgcaggg aaaacttacc tacggacttt acaggcacta cattagccgg
     3901 tcccggaagc tctgctacgg tataatgcat acgcacatca tccggcacat gctctcttaa
     3961 aaattcaata ccattaaatg ctactaaaag agcatcaaaa gcgctgcgtc cctgttccgg
     4021 agccagtgct gcatgggcac gttttccatg gaaagtaacc ctaaaggact ggtctgccag
     4081 gcactttaca tctgtacagg tagtcgggct tccatgcatc ataaacgatg catccagctc
     4141 gtggaaacag ccattcttga tcatatcaca cttagaacca agggtttcct ctgccggtgt
     4201 gccataaact accagtgtaa atggctcttc tcctgcacat tcctttaaag caactgctgc
     4261 gcctaaacag gacggtccct gcatatgatg gccgcatcca tgtcctaagc cctctaaagc
     4321 gtcatactca caaaggatac cgatcacagg accgccttcc ccatgtttcc actctgcacg
     4381 gaaggaagtc tcatatccac ctacgctttt ttctacagaa aacccattat ctgtaagata
     4441 ttttgttaaa agatcacatg cctgatattc tttgcctctg tattccggat ggtcaaagat
     4501 aaaatcagcc atttcccaca gtttttcttt ctgtgcttca attttttcaa ataattcatt
     4561 tttcgttgtc ataaaatttc cctccggaca ttacatcggt cctaaatgga taccctgggc
     4621 aattgctgtc ataatacatg ctgtaactgc ccagatcagg aacaatttcc acatgaactt
     4681 catccaacgg tcatatggga tgttaacagc actgatgata cccataagag cagtagaatg
     4741 aggcagaata tagttacaga aaccatcacc gaaattatat gcc
//
LOCUS       DBMDUA010000199         4761 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6722_length_4761_cov_1.903004, whole genome shotgun sequence.
ACCESSION   DBMDUA010000199 DBMDUA010000000
VERSION     DBMDUA010000199.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6722_length_4761_cov_1.903004"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="ACZPCL_11755"
     CDS             complement(<1..329)
                     /locus_tag="ACZPCL_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III N-terminal
                     domain-containing protein"
                     /protein_id="HIR1163177.1"
                     /translation="MAKGFLEVFPTLNIAEPLKELLAMAEVEKVTSARDRSSIRVYMK
                     STRLIHKQNIYDLERGIKDQLFPDKQITIKIQEKYRLSGQYTPEKLLKMYKDSLLLEL
                     KHYSIVE"
     gene            complement(335..1444)
                     /gene="ispG"
                     /locus_tag="ACZPCL_11760"
                     /gene_synonym="gcpE"
     CDS             complement(335..1444)
                     /gene="ispG"
                     /locus_tag="ACZPCL_11760"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR1163178.1"
                     /translation="MQSEIKNKEERNMSRKDTKVVHIGDRVIGGGNPILIQSMCNTKT
                     EDVKATVAQILSLEKAGCDIIRVAVPTMKAAEALKDIKKEIHIPLVADIHFDYRLAIA
                     AMENGADKIRINPGNIGSTDRVKAVVDRAKAGNIPIRVGVNSGSLEKDLVEKYGGVTA
                     EGIVESALDKVHLIEDMGYDNLVISIKSSDVLMCVKAHELITSRTDHPLHVGITEAGT
                     LFSGNIKSAVGLGIILYQGIGDTIRVSLTGDPIEEIKSARQILKTLGLRKGGVEVVSC
                     PTCGRTRIDLIHLANQVETMVSGMEGLDGVKVAVMGCVVNGPGEAREADIGIAGGVGE
                     GLIIKKGEILRKVPEEDLLDSLRSELEAMAEARKK"
     gene            complement(1465..2517)
                     /gene="rseP"
                     /locus_tag="ACZPCL_11765"
     CDS             complement(1465..2517)
                     /gene="rseP"
                     /locus_tag="ACZPCL_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009663542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR1163179.1"
                     /translation="MNIVLAVLILGGIILFHELGHFIFAKLSGIEVTEFSLGMGPRLL
                     SFVKGETRYSLKLLPFGGSCAMLGEDEDAEGDRAFNNKPVFNRIAVVFGGPLFNFILA
                     FLLSLVIVGCAGGFYEPVVMGVEEGYPAEQAGILTGDVIIKVDHRAVHSYRDLSPYLT
                     AHPHQDVTITWKHTDENGKTEKRSAKITPVYNDKTGQSMIGVRFEAKLQKITNPVQLL
                     VQSVYEVEHWIYYVFDSFHMIFAGRVSADDISGPVGIVTTIDQTVEQAAPAGNTVVAM
                     VLVNFAILLSANLGVMNLLPLPALDGGRLVFLIIEAIRRKPIDREKEGMVHAAGMFLL
                     LALMVFIMFNDVRKLL"
     gene            complement(2587..3738)
                     /locus_tag="ACZPCL_11770"
     CDS             complement(2587..3738)
                     /locus_tag="ACZPCL_11770"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR1163180.1"
                     /translation="MVKNISILGSTGSIGTQTLEVVRYNKDIKVTALAAGSNVDLIEK
                     QIREFKPEIACLWSEEKAKELKERVKDLDVKILSGMEGLIAVATEPKAEIVVTAVVGM
                     IGIRPTIAAMEAGKDIALANKETLVTAGHIIMPLAKKMGVKILPVDSEHSAIFQCLQG
                     AAGNKIHKILLTASGGPFRGWKREQLEKVQVEDALKHPNWSMGHKITIDSSTMVNKGL
                     EVMEAGWLFDVSLDQIQVVIQPASIIHSMIEYEDGAVIAQLGTPDMKLPIQYALYYPE
                     RRFLPGDRLDFTKLREITFDVPDPETFKGLKLAYQAGKTGGSMPTVFNAANEMAVSLF
                     LKKKIPYLTIPDMIQASMEHHKKVDAPDVDQILAAEAETYDFIHSKWNI"
     gene            complement(3809..4609)
                     /locus_tag="ACZPCL_11775"
     CDS             complement(3809..4609)
                     /locus_tag="ACZPCL_11775"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR1163181.1"
                     /translation="MFTTRLISGIVLVILAIFFVGKGGALLFAVTALISLVGLFELYR
                     VLGIEKRSVAAVGYLTAVCYYGILWFEGQKYLTLMIIASLMLLMALYVFTFPSFKTEE
                     ITGAFFGVCYVPVMLSFLYQTRSMHDGAYLVWLIFLSSWGCDTSAYCVGMLLGKHKLA
                     PVLSPKKSIEGAVGGVVGSALLGLAYGAYFGHNMEEVANPMVACAIACAIAAVISQIG
                     DLAASAIKRNHNIKDYGHLIPGHGGILDRFDSMIFTAPAIYFAVTFLR"
     gene            complement(4632..>4761)
                     /locus_tag="ACZPCL_11780"
     CDS             complement(4632..>4761)
                     /locus_tag="ACZPCL_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HIR1163182.1"
                     /translation="LWQLAYTELYVTDCLWPDFDEKELEKAIFQYNKRDRRFGGVK"
BASE COUNT         1254 a         1202 c          916 g         1389 t
ORIGIN      
        1 tattctacga tgctgtaatg ctttaattcc agcagcagac tgtccttata catttttaaa
       61 agcttttccg gtgtgtactg accagacagt ctgtattttt cctgtatctt aatggtgatc
      121 tgtttatccg ggaacagctg gtctttgatg ccccgctcca gatcatagat attttgttta
      181 tggatcagcc ttgtgctttt catataaaca cgaatagagc ttctatctct ggcagaagta
      241 actttttcta cttctgccat tgctaaaagc tcctttaaag gctctgcaat attaagggtt
      301 ggaaatactt ctaaaaaacc ttttgccatt cagattactt ctttcttgct tctgccattg
      361 cttccagttc actgcgaagg gagtctaaca gatcttcttc cggtactttt cgtaagatct
      421 cacctttttt gatgatcaga ccttcaccta cgccgcctgc aatgccaata tcagcttccc
      481 ttgcttcgcc aggtccattg actacgcagc ccataacagc tactttaaca ccgtctaagc
      541 cttccatgcc ggaaaccatg gtctctacct ggtttgcaag atggatcagg tcaatacggg
      601 tacggccgca ggttggacat gaaaccactt ctacgccgcc ttttctaagt cccagagttt
      661 taaggatctg acgggcagac ttgatctcct cgattggatc acctgtaagg gaaacgcgga
      721 tggtatctcc gatgccctga tataagatga tacccagccc cacagcagat ttgatatttc
      781 cggaaaataa agttccggct tctgtaatac ctacgtgaag aggatgatcg gttctggaag
      841 tgatcagttc atgtgctttc acacacatta atacatcgga agatttgata ctgatgacca
      901 gattgtcata gcccatatcc tcaataagat gtactttatc caaagcactt tctacgatac
      961 cttcagctgt tacaccaccg tatttttcca ccagatcttt ctcaagagat ccgctgttta
     1021 cacctacacg aatagggata tttcctgctt tcgcacggtc tactactgct tttacgcggt
     1081 ctgtacttcc aatattacct ggattgatac ggatcttgtc agctccgttt tccatagctg
     1141 caatcgccag acggtagtca aaatggatat ctgccactaa cgggatgtgg atctcttttt
     1201 taatatcctt aagggcttct gccgccttca tggtaggtac tgccacacgg ataatatcgc
     1261 agcctgcttt ttccaggctt aagatctgtg ctaccgttgc ctttacatct tctgtttttg
     1321 tattacacat agactggatg aggatgggat ttcccccgcc gatcacccgg tcaccaatat
     1381 gaacaacttt tgtatccttt ctgctcatgt ttctttcctc tttattctta atctccgatt
     1441 gcatgccgcc tggaggcagc acttttataa taattttctt acatcattaa acatgataaa
     1501 taccataagt gccagcagta aaaacatacc ggcagcatgg accattccct ctttttcacg
     1561 gtcaataggc tttctgcgta tagcctcgat gatcagaaat accagacgtc cgccatccag
     1621 tgcaggaagt ggaagcaggt tcatcacacc caggtttgcg cttaaaagaa tggcaaaatt
     1681 cacaagtacc atggcaacca ctgtatttcc tgcaggtgct gcctgctcca ccgtctggtc
     1741 aatggtagta acgatcccta cagggccact gatatcatct gcgcttactc tgccggcaaa
     1801 tatcatatga aaactgtcaa atacatagta gatccagtgt tccacctcat aaaccgactg
     1861 caccagcagc tgcaccggat ttgtgatctt ctgaagtttt gcctcaaacc gcacaccgat
     1921 catggactgc cctgtcttat cgttataaac cggagtgatc tttgccgatc tcttctctgt
     1981 tttcccattt tcatctgtat gtttccaggt aatggtcaca tcctgatgag gatgggctgt
     2041 aagataaggt gaaagatccc ggtaagaatg gaccgctctg tggtctactt ttatgatcac
     2101 atctcctgta aggatccccg cctgctcagc cgggtaccct tcttctacac ccatgaccac
     2161 cggctcataa aagccaccgg cacagcctac gataacaagg gataacagga aagccagaat
     2221 gaaattaaat aaagggcctc caaaaaccac ggcaatacgg ttaaatacag gtttgttatt
     2281 aaaagccctg tccccttctg catcctcatc ttctcctaac atggcgcagg aaccgccaaa
     2341 aggcagaagt tttaaagaat atctcgtttc gccttttaca aaacttaaaa gcctcggtcc
     2401 catccctaag gaaaattccg tcacttcaat cccgcttaat tttgcgaata taaaatgtcc
     2461 aagttcatgg aacaggatga ttcccccaag gatcagcact gccagtacaa tattcatagg
     2521 atttcattca tcctttcctg ttttcattta tggtaatatc accgccaggt gacatatccg
     2581 tttaacttaa atattccact ttgaatggat aaagtcataa gtttctgctt ccgctgccag
     2641 gatctgatct acatctggtg catcaacctt cttatgatgc tccatggatg cctggatcat
     2701 gtccggaatg gtcagatacg ggatcttttt ctttaaaaac agacttactg ccatctcatt
     2761 ggctgcatta aatacagttg gcatacttcc gcctgtcttt cctgcctggt atgccagttt
     2821 taatccctta aatgtttccg gatccggtac atcaaaggtg atctcacgaa gctttgtaaa
     2881 gtccagtctg tctcctggaa gaaaacgtct ttccggatag tataatgcat actggatcgg
     2941 cagtttcata tccggtgtac ctagctgggc gatcactgct ccatcttcat actcgatcat
     3001 ggaatggata atacttgcag gctggatcac tacctggatc tgatctaagg atacatcaaa
     3061 cagccagccg gcttccatta cttccagtcc cttattgacc atagtagagg agtcgatggt
     3121 gatcttgtgg cccatagacc agtttggatg ctttaaggca tcttccacct ggactttttc
     3181 cagctgttct cttttccatc ctctgaaagg acctccggaa gcagtcagaa ggatcttatg
     3241 gatcttattt ccggctgctc cctgaaggca ctggaagatt gcagaatgtt cggagtctac
     3301 cggaaggatc ttcacaccca ttttctttgc caacggcatg ataatgtgac cggctgtgac
     3361 cagtgtctcc ttatttgcca gagcaatgtc ttttcctgct tccatggcag cgatggtagg
     3421 ccggataccg atcattccca ctactgcggt aactacgatc tctgcctttg gctctgttgc
     3481 aacagcgatc aggccctcca taccggaaag gatcttcaca tccagatctt ttaccctttc
     3541 tttcagttct tttgcctttt cttcactcca caggcaggcg atctccggct taaattcgcg
     3601 gatctgtttt tcgatcaaat ccacattgga accggctgcc agagccgtta ccttaatatc
     3661 tttattgtat cttactacct ctaatgtctg ggttccgatg gaaccggttg aacccaggat
     3721 tgatatgttc tttaccataa ttattctccc tttgtaaagg aaatgcttaa aatcatgctg
     3781 ctccttttaa tttaaattgc ctatcttact atcttaaaaa agtaactgca aaataaatag
     3841 caggagctgt aaatatcata ctgtcaaaac gatccaggat acctccgtgt cccgggatca
     3901 gatggccata atctttaata ttgtggtttc tcttaatagc agacgctgcc agatctccaa
     3961 tctgtgagat aactgccgca atagcgcagg caatggcgca ggcaaccatg ggatttgcca
     4021 cttcttccat attatgacca aaataagcac cataagcaag gcctaacagt gcagaaccaa
     4081 ctacaccgcc tacagcccct tcaatggact ttttcggact taatacaggc gccagtttat
     4141 gttttccaag aagcattcct acgcagtagg cgctggtgtc gcagccccag gagcttaaaa
     4201 agatcagcca taccagataa gctccgtcat gcatgctgcg ggtctgatac agaaaagaaa
     4261 gcattaccgg aacatagcac acaccaaaaa aggcgccggt tatctcttct gttttaaagc
     4321 ttggaaaagt aaaaacatag agagccatta aaagcatcag ggatgcaatg atcattaatg
     4381 taagatactt ctgtccctca aaccataaga tcccataata gcagacagct gtcagatagc
     4441 caactgcagc tactgatctt ttttcgatcc cgagaacgcg gtacagttca aacaggccta
     4501 ccagtgaaat aagggctgtc actgcaaaaa ggagggcgcc tccttttcca acgaagaaaa
     4561 tagcaaggat gaccagtacg ataccactga tcaatcgtgt tgtaaacata aaaatctcct
     4621 ttttgaatac gttattttac cccgccgaac cgtcgatctc tcttattata ctgaaagatc
     4681 gccttttcca gttccttctc atcaaaatcc ggccacagac aatctgtaac atacagttct
     4741 gtataagcca actgccacag c
//
LOCUS       DBMDUA010000200         4756 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6730_length_4756_cov_2.847261, whole genome shotgun sequence.
ACCESSION   DBMDUA010000200 DBMDUA010000000
VERSION     DBMDUA010000200.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6730_length_4756_cov_2.847261"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            75..1235
                     /locus_tag="ACZPCL_11785"
     CDS             75..1235
                     /locus_tag="ACZPCL_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1163183.1"
                     /translation="MAITNGFEMVFNRRLIFGENKVTEIPGILNWYNKKKVLFVTFSA
                     EFDAFKKISSLLTDAGMAVVPYEVKTEPTLQIIDHGRDIYVAEGCDCTIALGGGSVVD
                     AAKVIGMLAVNGGDTEDYQMRGKAVTVEPPLFIAIPTTSGTGAEATKTSVVINNNNHL
                     KKSLYHNTMIADVVVLDPSLTLALPARITAATGMDALSHAIESYVSLNATPITEMYGL
                     KAIELVNKYLEEAYRNPDNLEARAGMMLASYFGGVAITAGIGIAHIMAQPLGGEYGIP
                     HGDACSIFLPASIILNQEYAEKKYVEISKAFGVYKEGASDKENVQALLDKITSLQKSI
                     DAPRCLKGYIKEEPSMDYLIDVIKRTTGHITCNPRPLNEELMKAAYELAMKD"
     gene            1381..2436
                     /locus_tag="ACZPCL_11790"
     CDS             1381..2436
                     /locus_tag="ACZPCL_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1163184.1"
                     /translation="MIYNDIKNDILNDVYKVGSLLPTEQVLSLKYDVNRSTLRKAMQM
                     LADEGLIEKCPGKGTIVLSSVPVAPESPKVITNKNIGFFLPKENIITEPFYSSLFNIL
                     ERDFQKNGCSLIYTTLDASDNIADKITALGLSGIVFVSNVVQKHIEYAVSQKIPCVLV
                     NSYSDKLPSVLSDNRKGAYLAGRYLIEHGHKTVALLAGVRSYLSNQERTAGFLQAFQE
                     EGLSIPPELILETGSWLYDAAEKVCLNFFNTYTGEMPTALFAFNDRLATGAINAICKT
                     GRTVPDDVSVIGYDNLGYYNIISPRITTVETHIEAISESTVMHMIWQLNNGSCLPVKV
                     LTPVEVISGETVRSLLC"
     gene            2569..4533
                     /locus_tag="ACZPCL_11795"
     CDS             2569..4533
                     /locus_tag="ACZPCL_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1163185.1"
                     /translation="MKLTEQTITHIIIGSGAAGFQAALRLYQNGERDLAIITENIKSG
                     TSRNTGSDKQTYYKLTLSGNDADSVRNMAEDLFAGQCVDGDQALCEAALSARCFFALT
                     ELGVPFPCTEHGEFMGYKTDHDRGRRATSAGPYTSKLMTEALERSVKEKQILILDQMQ
                     AIQILTYMNQVKGILCLDKNIHSEPAYKIIWCKNVILATGGPAGMYHDSVYPVSQTGS
                     TGMAFEAGASGKNLTEWQFGMASLNPRWNVSGTYMQVLPSFISTDQDGNDEKEFLLDY
                     FNELPDLLSMVFLKGYQWPFDVNKIFGGSSVIDLLVYQETVLKKRRVFLDYRVNPGNL
                     EKDRDLPYASMIPEAKEYLSQAGACFGTPIERLKHMNEPAILFYQDHHVDLFKERLEI
                     AVCAQHNNGGLSTNHLWETNLSGLYAIGEVCASHGVTRPGGTALNAGQVGAVRAAEGI
                     FLKKRTHMKEASPSTESGIKERLRIQALDRIRLSEHAEGNCPLSELWINASKRMSAAA
                     GMIRNQVQMETALKETEDDICQFMQKAKSPSVSQLSLFYKLYDMLLSQKMYLFAMLDY
                     AKAGGGSRGSALYTDSTGELPGFPGCQTFGELYRCRLDQKMHGQEVQEVTLKEGTPSA
                     SRRPVRPLPDVDYFFENQWRIYRERMGMMD"
     gene            complement(4598..>4756)
                     /locus_tag="ACZPCL_11800"
     CDS             complement(4598..>4756)
                     /locus_tag="ACZPCL_11800"
                     /inference="COORDINATES: protein motif:HMM:NF014326.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIR1163186.1"
                     /translation="VHTSLIQKEASGEKTEFSLILMHAEDQMASTEMAKVMAREFIEL
                     YREMKESR"
BASE COUNT         1452 a         1005 c         1008 g         1291 t
ORIGIN      
        1 tcaaatgtaa aaattcttct gacatctcaa caaatcaaca aacaaaacaa acacattttt
       61 aaggaggatg tatcatggca atcacaaatg gttttgaaat ggtttttaac agacgtctta
      121 ttttcggcga aaataaggta acagagatcc cggggatctt aaactggtac aacaagaaaa
      181 aagttctttt tgtaactttc agcgctgagt ttgatgcctt caagaagatc tcttctcttt
      241 taacagacgc cggtatggct gttgttcctt acgaagtaaa gactgagcca actcttcaga
      301 ttattgacca cggacgcgat atttacgttg cagaaggatg tgactgtacc attgctttag
      361 gcggcggaag tgttgttgat gcagcaaaag tgatcggtat gctggctgtt aacggcggcg
      421 acaccgaaga ctatcagatg agaggaaaag ctgttaccgt tgaaccacct ctgttcattg
      481 ctatcccaac tacctccgga accggtgctg aggctactaa gaccagcgtt gttatcaaca
      541 ataacaacca cttaaagaag agtctgtatc acaacaccat gattgctgat gttgttgttc
      601 tggatccaag cctgacttta gctcttcctg cacgtattac cgctgctaca ggaatggacg
      661 ctttaagtca tgctattgaa tcttacgttt ccttaaacgc aacacctatc accgaaatgt
      721 acggcttaaa ggcgattgaa ctggtaaaca aatatctgga agaagcttac cgcaatcctg
      781 ataatctgga agcaagagcc ggaatgatgc ttgcaagcta cttcggcggt gttgccatca
      841 ctgcaggtat tggtattgct cacatcatgg ctcagccttt aggaggcgaa tacggtattc
      901 ctcacggcga tgcatgctcc atcttcctgc cagcttccat tatcctgaat caggaatatg
      961 cagagaagaa atatgtagaa atctccaagg cattcggcgt atacaaagaa ggtgcttctg
     1021 acaaagaaaa tgtacaggct ctgttagaca aaattacttc tctgcagaaa tccatcgatg
     1081 ctcctcgctg cttaaaggga tatataaagg aagaaccaag catggattat ctgatcgatg
     1141 tgatcaagag aactaccgga catattacct gcaatccacg tcctctgaat gaggaactga
     1201 tgaaagcagc atatgagctg gctatgaaag actgatcggc aggtttttaa aacatgaatt
     1261 gcaggaaaga gctgaacatg ttaaaatgtt aaatgtatac tttatgtata aatattagtc
     1321 aacaaattaa caaacgattc agttaggtaa aatatgagca aagaaaaatt tcgttattcc
     1381 atgatttata atgacatcaa aaatgatatc ctgaatgatg tctacaaggt aggaagcctg
     1441 ctccccacag agcaggtcct ctccctgaaa tacgatgtca accgcagcac tcttcgaaaa
     1501 gcaatgcaga tgttagcaga cgaagggctg atcgaaaaat gccccggaaa aggcactatt
     1561 gttttaagca gtgtcccggt tgccccagag tccccaaaag ttatcaccaa caaaaacatc
     1621 ggttttttcc tcccaaaaga aaatattata accgaaccat tttactcttc cctgtttaat
     1681 atactggaac gtgattttca gaaaaacggc tgttccctga tctacactac actggatgct
     1741 tccgataata ttgcagataa gattacagca cttggtcttt ccggaatcgt ttttgtaagt
     1801 aatgttgttc aaaaacacat tgaatatgct gtcagtcaga aaataccctg tgttttagtc
     1861 aacagctact ccgacaagct tccttctgtt ttatcagata accgcaaagg agcttatctt
     1921 gcaggcagat atcttatcga acacggacac aaaactgtcg ctttactggc aggtgtgcga
     1981 agctatttaa gtaaccagga acgtaccgcc ggatttctcc aggctttcca ggaagaagga
     2041 ctctctattc ctcctgaatt gatcttagaa acaggctcct ggctttatga cgcagctgaa
     2101 aaggtgtgct tgaacttttt caacacttac acaggcgaaa tgcctactgc cctgtttgca
     2161 tttaatgacc gtttggcaac tggagccatc aatgccatct gcaaaaccgg tcgtactgta
     2221 cctgacgatg taagtgtgat cggctatgat aatctgggct actataatat tatcagccca
     2281 cggatcacta cagtggaaac acatatcgaa gctatctccg aatctacggt aatgcatatg
     2341 atatggcagc taaacaacgg cagttgtctg cctgtaaaag tccttacccc agttgaagtg
     2401 atctccggcg aaaccgtccg ttctttactt tgctaaattt tacatctgca attcattttt
     2461 atatcttttt gtcaaaaata aaaacgccca gaatagtttt cggatcttct ttctgattat
     2521 tctgggcgtt ttttactgtg atctcttatg atcagaaagg gagacaagat gaagctaact
     2581 gaacaaacga ttacacatat tatcatcgga agtggagcag caggatttca ggccgcatta
     2641 cgcttatatc aaaacggtga aagagatctg gcaatcataa cagaaaatat aaaatcaggt
     2701 acttccagaa ataccggttc agacaagcag acttattata agcttacatt gtcaggaaac
     2761 gatgctgaca gcgtaagaaa tatggcagaa gacctgtttg caggacaatg tgtagacgga
     2821 gaccaggcat tatgtgaagc ggctctttct gcccggtgtt tttttgccct tacagagctg
     2881 ggagttcctt ttccctgcac agagcacggt gaatttatgg gatataaaac agatcatgac
     2941 agaggaagaa gagccaccag tgccggtcct tatacttcta agcttatgac ggaagccctt
     3001 gaaagatctg taaaagagaa acagatccta attttggatc agatgcaggc tattcagatc
     3061 cttacataca tgaaccaagt aaagggaatc ctctgcctgg acaaaaacat tcatagcgaa
     3121 cctgcttata aaatcatctg gtgtaaaaat gtgatcctcg caacaggcgg accagctggc
     3181 atgtatcacg acagtgtgta tcccgtttcg caaaccggct ccacaggtat ggcatttgaa
     3241 gctggtgctt ctgggaaaaa cctgacagaa tggcaatttg gaatggcttc tttaaatcca
     3301 agatggaatg taagcggaac atatatgcag gtacttccaa gctttatttc tactgaccag
     3361 gacggaaatg atgaaaaaga atttcttctt gattatttta atgaactgcc agatctgctt
     3421 tctatggttt ttttgaaagg ttatcaatgg ccatttgatg taaataaaat ttttggcggc
     3481 tcttcggtga tcgacctatt ggtttaccag gagactgtat taaaaaaacg cagagtattt
     3541 ttggattaca gggtcaatcc aggaaacctt gaaaaagaca gggatcttcc ttatgcatct
     3601 atgatcccgg aagcaaaaga atatttaagc caggccggag cctgcttcgg aacacccatt
     3661 gaacgtttaa agcatatgaa cgagcctgct attctttttt atcaggatca ccatgtagat
     3721 ctgtttaaag aacgtttgga gattgccgta tgtgcccaac ataataacgg tggtctgtcc
     3781 accaaccatc tatgggaaac caatctctct ggtctttacg cgatcggaga agtgtgtgca
     3841 agtcatggag ttacaagacc tggcggaact gctttaaatg caggtcaggt aggtgctgtc
     3901 cgggcggctg aaggaatttt tcttaaaaaa agaacacaca tgaaagaagc gagcccttcc
     3961 accgagagtg gtataaaaga aaggctgcgc attcaggcgc tggatcggat cagactttca
     4021 gaacatgcag aaggaaactg cccattgtcc gaactgtgga taaatgcctc taaaagaatg
     4081 agtgccgctg caggtatgat ccgcaaccag gtgcaaatgg aaacagcttt aaaagaaaca
     4141 gaggatgaca tatgtcaatt tatgcaaaaa gcaaaatcgc cttctgtttc acaactttca
     4201 ttgttctaca agctgtacga tatgcttctt tctcaaaaaa tgtatctatt tgccatgtta
     4261 gactacgcaa aagcaggcgg aggaagccga ggttccgcat tatatacaga ttccacagga
     4321 gaactcccag gctttccagg ctgccaaacc tttggagaat tatatcgctg cagactggac
     4381 cagaaaatgc atggccagga agtccaggaa gtaactttaa aagagggaac gccgtctgcc
     4441 tcaagacgcc ctgtacgtcc attacctgat gttgactatt tctttgaaaa tcaatggaga
     4501 atatacagag aacggatggg tatgatggat taaaagtcaa aaatacccag gcgatcgttt
     4561 cccgaacccg cctgggtatt tcctattcgc agatacctta tctgctttcc ttcatctctc
     4621 tatataattc aataaactcc cttgccatta ccttggccat ttccgtactt gccatctgat
     4681 cttctgcatg catcaggatc agggaaaact ctgttttttc accggaagct tctttctgta
     4741 ttaaagaagt gtggac
//
LOCUS       DBMDUA010000201         4705 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6803_length_4705_cov_2.514553, whole genome shotgun sequence.
ACCESSION   DBMDUA010000201 DBMDUA010000000
VERSION     DBMDUA010000201.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6803_length_4705_cov_2.514553"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /gene="hisG"
                     /locus_tag="ACZPCL_11805"
     CDS             complement(<1..342)
                     /gene="hisG"
                     /locus_tag="ACZPCL_11805"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR1163187.1"
                     /translation="MRYLTIALAKGRLASKAMDMFEQIGISCEEMKDKNSRKLIFVNE
                     DLGIRFFLAKANDVPTYVEYGAADIGIVGRDTILEEGRRLYEVLDLAMGKCRMCVCGP
                     ESARERLKHHEL"
     gene            complement(394..1674)
                     /gene="hisZ"
                     /locus_tag="ACZPCL_11810"
     CDS             complement(394..1674)
                     /gene="hisZ"
                     /locus_tag="ACZPCL_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR1163188.1"
                     /translation="MTKKNKLIHTPEGVRDIYNGECRRKLAVQEKILHTLYLYGYEHI
                     QTPSFEYADIFNEDRGSVGAQEMFKFFDRDNNMLVLRPDMTPAVARCVAKYFMDDDMP
                     LRLCYLERTFKNNTSYQGYLKERAESGAELIGDDSPDADAEMIAMVIDCLKAAGLKEF
                     QVELGEVAFYRSLLQQSGLDEDAQAQLNDLIENKNRFGVEEFLKNQDMEESLKEAFLK
                     LPELFGGINQIKKAKALTDNPQALAAIERLERVHTLLEEYHMADYVSYDLGMLSRYQY
                     YTGIIFKAYTYGTGDYIVTGGRYNKLLVQFGKDTPAVGFAIVVDQLMAALSRQQIHIP
                     VQLVNTVILYEPSARQSAVRLSRFFRSEGTPVQSMVMEEGKSLEDYKGMAKKRGLRTI
                     LCLKDTGNTVTAFDMLTGNVDETPVSAYGVREIL"
     gene            complement(1840..2382)
                     /locus_tag="ACZPCL_11815"
     CDS             complement(1840..2382)
                     /locus_tag="ACZPCL_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009214715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HIR1163189.1"
                     /translation="MGTWKTRGLRGSTLEDLINHTNESYREKKLALIQKIPTPITPIE
                     IDKSSRHITLAYFDQKSTVDYIGAVQGIPVCFDAKECAVKTFPLQNIHPHQIKFMEEF
                     EQQGGISFIIVHYTALDEMYYVPFTDVKKFWDRMEAGGRKSFTYDEIDKNWKIRSHRD
                     MYVHYLEPLSIDLSQRENDQ"
     gene            2671..3801
                     /locus_tag="ACZPCL_11820"
     CDS             2671..3801
                     /locus_tag="ACZPCL_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005346593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR1163190.1"
                     /translation="MEPSKVYFTDFHTVAFGDGLPTKLKKLIKKAGIEKLDMDGKFVA
                     IKMHFGELGNISYLRPNYAKAVADVVKEFGGKPFLTDCNTMYPGSRKNALEHLECAWE
                     NGFTPLTVGCPILIGDGLKGTDDVDVPVAGGEYVKEAKIGRAIMDADVFISLTHFKGH
                     EMTGFGGTIKNIGMGCGSRAGKKDQHSSGKPHIEQDVCKGCRKCQKECANGGLIFDET
                     ARKMHVDQEHCVGCGRCLGACNFDAIAFDNDTANELLNCRMAEYTKAVVDGRPNFHIS
                     LVVDVSPNCDCHGENDVPILPNIGMFASFDPLALDQACVDACLKATPMPGSQLAKNMA
                     KKGFVDHHDHFCNSTPESEWRSCLEHAEKIGLGSRKYELIIM"
     gene            complement(3905..>4705)
                     /locus_tag="ACZPCL_11825"
     CDS             complement(3905..>4705)
                     /locus_tag="ACZPCL_11825"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583549.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR1163191.1"
                     /translation="DLVTVSRLQRNPTVKTEIQMRNFETSIPVGFFTYPISQAADITA
                     FKATTVPVGEDQAPMIEQTREIVHKFNTVYGGNLVEPEILLPDNKACLRLPGIDGKAK
                     MSKSLGNCIYLSDSEEEIKKKIMSMFTDPNHLRVQDPGQVEGNPVFIYLDAFCRDEHF
                     EKYLPDYKNLDELKAHYMRGGLGDVKVKKFLNNVIQDELRPIRERRKEIAKDIPAVYK
                     ILEEGSIKAEKKAAQTLAEMKRAMKINYFEDKELIAEQAARFSSEADA"
BASE COUNT         1179 a         1120 c          981 g         1425 t
ORIGIN      
        1 cagttcgtgg tgttttaaac gctctcttgc tgactccggt ccgcagacgc acatcctgca
       61 ttttcccatt gccaggtcta agacctcata cagtcttctg ccttcctcta aaatggtgtc
      121 acggcctaca ataccgatgt ctgccgcgcc atattctaca taagtaggaa cgtcatttgc
      181 cttggctaag aagaaacgga tgcccaggtc ttcatttaca aagatcagct ttctggagtt
      241 tttgtccttc atctcctcac aggaaatgcc gatctgttca aacatatcca ttgccttgga
      301 tgccagacgg cccttggcta atgctatggt cagatatctc atgctgcttt atctcctttt
      361 atatccaata tgcttttatg ctttctggat cttttataag atctccctga ctccgtatgc
      421 agatacaggg gtttcgtcta catttcctgt aagcatgtca aaggcagtta ctgtatttcc
      481 tgtatccttc aggcagagaa tggtgcgaag acctctcttc tttgccattc ccttataatc
      541 ttccaagctc tttccttctt ccatgaccat ggactgcacc ggagtaccct cggaacggaa
      601 gaaacggctt aaacgtaccg cactttgtct ggctgagggc tcatacagga tcaccgtatt
      661 tactaactgc accggaatgt ggatctgctg tctggataaa gctgccatca gctggtctac
      721 tacaatggcg aaaccaactg ccggagtatc ttttccaaac tggactaaca gtttattgta
      781 acggccgccg gtaacaatgt aatcgccggt tccataggta tatgccttga aaatgatacc
      841 ggtataatac tggtaacggc ttaacattcc caggtcgtaa gatacataat cagccatatg
      901 atattcttcc agcaaagtat gtacccgctc cagccgctca atggctgcta atgcctgtgg
      961 gttatctgtt aatgcttttg cctttttaat ctggttgata ccgccaaaca gctctggaag
     1021 ttttaaaaac gcttctttta agctttcttc catgtcctgg ttctttaaaa actcttctac
     1081 gccaaagcgg ttcttgtttt caatcagatc atttaactgg gcctgtgcgt cctcgtccag
     1141 tccggactgc tgtaacaggc ttctgtaaaa tgcaacctca cctaattcta cctggaactc
     1201 ttttaaacca gctgccttta aacagtcaat gaccatagcg atcatctcag catctgcatc
     1261 tggtgagtca tcgccgatca gctctgcacc tgactcagcc cgctctttta aataaccctg
     1321 atagctggta ttatttttaa aagtgcgctc caggtagcac agacgcagag gcatatcgtc
     1381 atccataaaa tattttgcca cacatcttgc cactgctggg gtcatatccg gccggagcac
     1441 cagcatgttg ttgtccctgt cgaagaactt gaacatttcc tgggcgccta cgcttccgcg
     1501 gtcttcatta aaaatatccg catattcaaa acttggggtc tggatatgct cataaccgta
     1561 aagatacaat gtatggagga ttttctcctg aactgccagc tttctgcggc attctccgtt
     1621 ataaatgtcc ctgactcctt ccggagtgtg gatcagtttg tttttctttg tcatggcaca
     1681 tctcccttat cttttgccga tataaatggc tttgcttttt gtcttgctat cactttacca
     1741 tggtagagtg atgatttgct aacgtgtttt cattataaag gcttcattct ttcttgtcaa
     1801 cggctttctg gatttttctt agccaggctc atggaacggc tattgatcgt tctccctctg
     1861 acttagatca atggataatg gttccaggta atggacatac atatccctat gagaacgtat
     1921 tttccagttt ttgtcaattt catcgtaggt aaaacttttt ctgccacctg cttccatgcg
     1981 gtcccagaat ttttttacat ctgtaaaagg cacataatac atttcatcta atgctgtata
     2041 atgaacaatg ataaatgata tccccccctg ttgttcaaat tcttccataa atttgatctg
     2101 atgtgggtga atgttctgaa gaggaaatgt ttttaccgca cactcttttg cgtcaaaaca
     2161 taccggtatt ccttgaactg ccccaatata gtctacggta ctcttctggt caaaataagc
     2221 cagtgtaata tgacggctgc tcttatctat ttcaatggga gtaatgggtg tagggatctt
     2281 ctggatcaga gccagttttt tctccctgta actttcattt gtatgattga tcaggtcttc
     2341 taaggtagaa ccccgaagcc cccttgtttt ccatgttccc attttatgct gtcctttccg
     2401 caaaacaaat gtctgctgcg cagccgcttg ttttatcttc gctcaaatac tcacgaatcc
     2461 cgcgctatcg cgctccactg tctgctttcg cagacgattc gttcgttaat ggctcgccaa
     2521 aaacaaatgt ctgctgcgca gccgcttgtt tttgtcttca ctcattacat tatatcaaat
     2581 acaattcata tttgccattc ttcatttttt gcactatact atagagaaaa aatatcactt
     2641 aaatataatc acacaaaagc gaggtattgt atggaaccat caaaagttta ttttactgat
     2701 tttcacaccg ttgcctttgg tgacggactt ccaaccaagt taaagaagct gatcaaaaaa
     2761 gccggtatcg aaaaactgga tatggacggc aaatttgttg ctattaagat gcacttcgga
     2821 gaactgggaa atatcagtta tcttcgtcct aactatgcaa aagcggttgc agacgtagta
     2881 aaggagtttg gcggaaagcc atttcttact gactgtaata ccatgtatcc aggaagccgc
     2941 aaaaacgctt tagagcacct ggaatgtgcc tgggaaaatg gatttacacc gctgactgta
     3001 ggatgtccta tccttattgg tgacggtctg aaaggtacag atgatgtaga tgtgccggta
     3061 gcaggcgggg aatatgtaaa agaagcaaag atcggccgtg cgatcatgga tgctgacgta
     3121 tttatcagcc tgactcattt taaaggccat gaaatgactg gttttggcgg aactatcaaa
     3181 aacatcggta tgggctgcgg ttccagagcc ggaaagaaag accagcacag cagcggcaaa
     3241 ccacacattg agcaggatgt ctgcaaaggc tgccgcaaat gccagaagga atgtgccaac
     3301 ggcggtctga tctttgatga aactgccaga aaaatgcatg tagatcagga acactgcgta
     3361 ggctgcggcc gctgcctggg tgcctgcaac ttcgatgcca ttgcatttga caacgatact
     3421 gcaaatgaac tgttaaactg ccgtatggct gagtatacca aagcggttgt agacggccgt
     3481 ccaaacttcc atatttccct ggtagtagat gtatccccta actgcgactg ccatggtgaa
     3541 aatgacgttc cgatccttcc aaatattgga atgttcgcct ccttcgaccc gctggcactg
     3601 gatcaggcct gcgtagacgc ctgcctcaaa gccaccccaa tgccaggaag ccagcttgca
     3661 aagaacatgg caaaaaaagg tttcgtagac caccacgatc acttctgcaa ctctactcca
     3721 gagtctgagt ggagaagctg cctggaacat gcagaaaaga ttgggcttgg ttcaagaaag
     3781 tatgaattga tcattatgta aatacagtta tggaaaaccg cccggacttg atcggtccgg
     3841 gcggtttttt catatgacct tatataaaaa cttatacgat cttttatcgc tttttcagat
     3901 atacttacgc atctgcctca ctggaaaatc tagctgcctg ctctgcgatc agctccttat
     3961 cttcaaagta gttgatcttc atagcgcgct tcatctctgc aagggtctgt gctgctttct
     4021 tttctgcctt gatgcttccc tcttccagga tcttgtatac agctggaatg tcttttgcga
     4081 tctcttttct gcgttctctg atcgggcgaa gttcatcctg gatcacgttg tttaagaact
     4141 tcttaacctt aacgtctcca aggccgcctc tcatataatg agcctttaat tcatccaggt
     4201 tcttgtaatc tggcagatat ttttcaaagt gctcatcacg gcagaatgca tccagataga
     4261 taaatactgg atttccttct acctgtcccg gatcctgaac gcgcaggtgg ttcgggtctg
     4321 tgaacatgga catgatcttc ttcttgattt cttcttcgga gtcggacaga tagatgcagt
     4381 ttcctaagga cttgctcatt tttgcctttc cgtcgatacc tggcagacgc agacatgctt
     4441 tattgtctgg aagcaggatc tcaggctcta ccagattgcc gccgtatacg gtattaaact
     4501 tgtgaacgat ctcacgagtc tgctcgatca ttggagcctg gtcttcacca accggaacgg
     4561 tggttgcctt aaatgcagta atatctgctg cctggctgat cgggtaggta aagaaaccaa
     4621 ctgggatgct ggtctcgaaa ttacgcatct ggatctcggt ttttacagta ggattacgct
     4681 gtaatctgga tacagttacc agatc
//
LOCUS       DBMDUA010000202         4633 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6916_length_4633_cov_3.153354, whole genome shotgun sequence.
ACCESSION   DBMDUA010000202 DBMDUA010000000
VERSION     DBMDUA010000202.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6916_length_4633_cov_3.153354"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(3..2912)
                     /gene="infB"
                     /locus_tag="ACZPCL_11830"
     CDS             complement(3..2912)
                     /gene="infB"
                     /locus_tag="ACZPCL_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR1163192.1"
                     /translation="MRVSELANELGKTSKEVLDIIKQKDSSAALFAASSVSKDQENMV
                     RNAVSQKKEGKTEPAKKKLTAVFRPQNAQQQIKRPVPKQQPKPQQAPAKAEETKPVQA
                     AEVKPQVQEASAPAAESRPAQAERGDFQGRSQNGYQGRDNRGERPQGGYQNRDGRNGS
                     YQGRDNNRGERPQGGYQNRDGRNGGYQGRDNNRGDRPQGGYQNRDGRNGGYQGRDNRG
                     ERPQGGYQNRDGRPQGGGYQNRGDRPQGGFQNRGDRPQGVGYQNRGDRPQGGYQNRDG
                     RPQGGFQNRDRNQGGFGSRFGEDKDKDNTSRNGSRDNRRSSGNRDNRSSAFDTPIQTK
                     PSSTRQANKNAHKNDRFDKRDREEDGKKKAPKTGKGAFIMPQPKKEEAKVEDVKTITL
                     PERLTIKELADKMKLQPSVIVKKLFLKGQMVTLNQEIDYDQAEEIAMEFDVLCEQEEK
                     VDVIAELLKEEDENEADMVSRPPVVCVMGHVDHGKTSLLDAIRQTNVTAREAGGITQH
                     IGASVIEINGRKITFLDTPGHEAFTAMRMRGAQSTDIAILVVAADDGVMPQTVEAINH
                     AKAAGVEIIVAINKIDKPSANIDKVKQELAEYELIPEDWGGSTIFVPVSAHTKEGITD
                     LLEMVLLTADVLELKANPNRKGRGLVIEAELDKGKGPVATVLVQKGTLRVGDTIAAGA
                     CYGKIRAMMDDKGRRVKEAGPSTPVEILGLNDVPQAGEVFVATENEKEARSFAETFIS
                     ESKNKLLEDTKAKLSLDDLFSQIKAGNVKELPLIIKADVQGSVEAVKQSLTKLSNEEV
                     VVKVIHGGVGAINESDVSLASASNAIIIGFNVRPDATAKSIAEREKVDVRLYKVIYQA
                     IEDVEAAMKGMLDPVFEEKVIGHAVIRQLFKASGVGTIAGSYVLDGKFQRGCSCRITR
                     EGEQIYDGPLASLKRFKDDVKEVAAGYECGLVFEKFNDLQEDDQIEAYMMVEVPR"
     gene            complement(2899..3207)
                     /locus_tag="ACZPCL_11835"
     CDS             complement(2899..3207)
                     /locus_tag="ACZPCL_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L7Ae/L30e/S12e/Gadd45 family ribosomal protein"
                     /protein_id="HIR1163193.1"
                     /translation="MIGLATRARKVVSGEFSTEKSVKSGRSHMVIVSEEASDNTKKMF
                     TNMCTHYKVPIYLFGTKEELGHAMGQEFRASLSVEDAGFAKTLASRMNVNGGSLNESK
                     "
     gene            complement(3218..3496)
                     /gene="rnpM"
                     /locus_tag="ACZPCL_11840"
                     /gene_synonym="ylxR"
     CDS             complement(3218..3496)
                     /gene="rnpM"
                     /locus_tag="ACZPCL_11840"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HIR1163194.1"
                     /translation="MSVKKIPLRQCIGCGEMKSKKEMIRVIKTAEGEILLDATGRKNG
                     RGAYLCPSMECFKKAVKGRGLERSFKMAIPREVYETLEKEMEEIDRQE"
     gene            complement(3530..>4633)
                     /gene="nusA"
                     /locus_tag="ACZPCL_11845"
     CDS             complement(3530..>4633)
                     /gene="nusA"
                     /locus_tag="ACZPCL_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR1163195.1"
                     /translation="AIENSLITACKNNFGKADNVKVVMNRTTGDFAVYAEKTVVEEVE
                     DPVMEISLADARMISAKYQLGDTVQVPLDSKKFGRIATQNAKNVILQKIREEERKALY
                     NDYYTMEKDVATGIVQRYLGKNISVNLGKLDAILNESEQVKGETFRPNERIKVYIVEV
                     KDTTKGPRISVSRTHPDLVKRLFEEEVSEVHDGTVEIKAIAREAGSRTKIAVKSNDPS
                     VDPVGACVGVNGARVNAIVNELRGEKIDIINWDENPAYLIENALSPAKVICVVADEEA
                     REAQVIVPDYQLSLAIGKEGQNARLAARLTGFKIDIKSETQAKEMGLFEQMGLQYGDA
                     PAPSYEEENAEDAEQEATEEPAQDDYQEENNEQ"
BASE COUNT          958 a         1264 c          934 g         1477 t
ORIGIN      
        1 gactatctag gaacctctac catcatataa gcttcgatct ggtcatcttc ctgaagatca
       61 ttgaacttct cgaatacaag accacactca tatcctgcag caacttcctt tacatcgtcc
      121 ttgaaacgct ttaaggaagc taaaggtcca tcgtagatct gctcgccttc tcttgtaata
      181 cggcagctgc agcctctctg gaacttgcca tccagtacat aggaacccgc gatggtgcca
      241 acaccggaag ccttgaacag ctgacggatc actgcatgac cgatcacctt ctcctcgaat
      301 acaggatcca gcataccctt catagcagct tctacatctt cgatggcctg gtagatgacc
      361 ttgtacagac gtacatctac tttctcacgc tctgcaatgg attttgcagt tgcatcagga
      421 cgtacattga aaccaatgat gatggcattg gaagcagaag ccagggaaac gtcagactca
      481 ttgatagcac caacgccgcc gtggattacc tttactacta cctcttcatt agacagcttg
      541 gtcaggctct gctttactgc ttctacagaa ccctgaacgt ctgccttgat gatcagagga
      601 agttccttta catttcctgc cttgatctgg ctgaacagat catccagaga cagttttgcc
      661 ttagtatctt ccagaagctt gttcttgctc tcagaaatga aggtttctgc aaagcttctt
      721 gcttctttct cgttctcagt tgctacgaat acctcaccag cctgtggaac atcatttaaa
      781 cctaagatct ctacaggagt ggatggacca gcctctttta ctctgcggcc cttatcatcc
      841 atcatagcac ggatcttacc atagcatgca cctgcagcaa tggtgtcacc tactcttaag
      901 gtacctttct gtaccagtac agttgcaaca ggtcctttgc ccttatccag ctcagcctcg
      961 atcacaagtc cacggccctt acggtttgga tttgccttta attcaagaac atctgctgtt
     1021 aaaagtacca tttccagaag gtctgtgata ccctcttttg tatgtgcgga tacaggaacg
     1081 aaaatagtgc tgccgcccca gtcttccggg atcagctcat attcagccaa ttcctgtttt
     1141 actttgtcaa tgtttgcact tggcttatcg atcttgttga tagcaacgat gatctctacg
     1201 ccagctgcct ttgcatggtt gatcgcctca acagtctgtg gcataacgcc gtcgtctgct
     1261 gctactacca ggatagcgat atcagtagac tgggcacctc tcatacgcat agctgtaaat
     1321 gcctcatgtc caggtgtatc aaggaaagtg atctttctgc cgttaatctc aataacagaa
     1381 gcaccgatgt gctgggtgat accgcctgcc tctcttgcag ttacgttagt ctgacggatg
     1441 gcatccagaa gggatgtttt accatggtca acgtgaccca ttacgcagac tacaggaggt
     1501 ctggaaacca tatctgcctc gttttcgtcc tcttctttca gaagttcagc aattacgtct
     1561 accttttctt cctgctcgca gagaacgtca aattccattg cgatctcttc tgcctgatcg
     1621 tagtcgatct cctggtttaa ggtaaccatc tgtcccttta agaacagctt cttaacgata
     1681 acggatggct gcagcttcat cttatcagcc agttccttaa tagttaagcg ctctggaagg
     1741 gtaatggtct taacatcctc aaccttagcc tcttctttct taggctgtgg catgatgaaa
     1801 gctccctttc ctgtctttgg tgctttcttc tttccgtctt cttctctgtc tctcttgtca
     1861 aaacggtcat tcttatgagc gttcttgttt gcctgacggg tgctgcttgg cttggtctgg
     1921 atcggggtat caaaagcaga tgaacggttg tctctgtttc cagatgaacg gcggttgtct
     1981 ctgctgccat ttctgcttgt attgtcctta tccttatctt ctccgaaacg ggagccaaag
     2041 ccgccctgat ttctgtcacg gttctggaaa ccgccctgtg gtctgccatc gcggttctga
     2101 tagccgccct gtggtctgtc tccacggttc tgatagccaa ccccctgtgg tctgtcccca
     2161 cggttctgga agccaccctg tggtctgtct ccacggttct gatagccgcc gccctgtggt
     2221 ctgccgtcac ggttctgata accgccctgt ggtctctcac cacggttatc tcttccctga
     2281 tagccgccgt ttcttccatc acgattctgg tagccgccct gtggtctgtc tccacggtta
     2341 ttatctcttc cctggtagcc gccgtttctt ccatcacggt tctgatagcc gccctgtggt
     2401 ctctcaccac ggttattgtc tcttccctgg tagctgccgt ttcttccatc acggttctga
     2461 taaccgccct gtggtctctc accgcggtta tctcttccct ggtagccgtt ctggcttctg
     2521 ccctggaaat ctcctctttc agcctgtgca ggtctgctct ctgctgccgg agcagatgct
     2581 tcctgtacct gtggctttac ttctgccgcc tgtaccggtt tggtctcttc tgccttagca
     2641 ggtgcctgct gtggctttgg ctgctgcttt ggtaccggac gcttgatctg ctgctgtgcg
     2701 ttctgcggac ggaatacagc tgtcagtttc ttctttgcag gctctgtctt tccctctttc
     2761 ttctggctaa cagcatttct taccatattt tcctggtcct tagatacgct ggaagctgca
     2821 aaaagagctg cagagctgtc tttctgttta ataatatcta atacttcttt actggtctta
     2881 cccagttcat ttgccagttc acttactctc attcaaacta cctccattta cattcatccg
     2941 tgatgccagg gtcttggcaa atcctgcgtc ctctactgaa agggaagcac gaaattcctg
     3001 tcccatagca tgcccaagtt cctcttttgt gccgaacaga tagatgggaa ctttatagtg
     3061 agtacacata ttggtaaaca ttttctttgt attatcagaa gcttcttctg atacgatcac
     3121 catatgggaa cgtccgcttt taacggattt ctctgtagaa aattctccgc tgaccacttt
     3181 tctcgctctg gtcgccagtc cgatcatact aaatacttta ttcttgtctg tcaatttctt
     3241 ccatctcctt ttccagagtc tcataaactt ctctggggat cgccatttta aaggatcgtt
     3301 ccaggcctct gcctttcaca gccttcttaa agcactccat agacgggcac agataagcac
     3361 cccggccgtt tttacgtccg gtagcatcta ataagatctc accttcggct gttttgatca
     3421 ctctaatcat ttctttttta cttttcatct caccgcagcc aatgcactgc ctcagcggga
     3481 tcttctttac actcaccttt gatcactcct catcttccgg aaaacctttt tactgttcgt
     3541 tgttttcttc ctggtagtca tcctgagctg gctcttctgt tgcttcctgc tctgcatctt
     3601 ctgcattctc ttcctcataa gaaggagccg gagcatcacc gtactgtaag cccatctgct
     3661 caaacaggcc catttccttt gcctgtgtct cgctcttgat atcgatctta aatccagtca
     3721 gtcttgctgc aagacgtgca ttctgtccct ctttaccgat tgccagtgat aactgataat
     3781 caggaacgat gacctgtgct tctcttgctt cttcgtctgc aacaacgcag ataacctttg
     3841 ccgggcttaa tgcgttttcg atcaggtatg caggattttc atcccagttg atgatatcga
     3901 tcttctcacc gcgcagttcg tttacgatgg catttactct ggcaccgttt acgcctacac
     3961 atgcgcctac tggatctaca cttggatcat tggacttaac tgcgatcttg gttctggaac
     4021 ctgcttctct tgcgatggcc ttgatctcta cagtaccatc atgtacttca gatacttcct
     4081 cttcaaacag acgctttaca aggtctggat gggttctgga tacggaaata cgaggtccct
     4141 ttgtagtatc ctttacttct acgatgtaaa ctttgatacg ctcatttgga cggaaagtct
     4201 caccctttac ctgctcactc tcatttaaga tcgcatctaa cttgccaagg ttaacactga
     4261 tattttttcc aaggtaacgc tgtacaatac cggtagcaac gtctttttcc atggtgtagt
     4321 aatcattgta taatgccttg cgctcttctt cgcggatctt ctgaaggata acgttctttg
     4381 cgttctgggt agcgatacgg ccgaatttct tggaatctaa aggaacctgt acggtatcac
     4441 caagctggta ttttgcgctg atcattcttg catctgccag gctgatctcc ataaccggat
     4501 cttctacctc ttctactacg gtcttctctg cgtatacagc aaagtcaccg gttgtacggt
     4561 tcataacaac ttttacattg tcagccttgc cgaaattgtt tttacaggca gtgataaggg
     4621 aattttcaat tgc
//
LOCUS       DBMDUA010000203         4622 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_6936_length_4622_cov_2.902455, whole genome shotgun sequence.
ACCESSION   DBMDUA010000203 DBMDUA010000000
VERSION     DBMDUA010000203.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6936_length_4622_cov_2.902455"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(51..1310)
                     /locus_tag="ACZPCL_11850"
                     /pseudo
     CDS             complement(51..1310)
                     /locus_tag="ACZPCL_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568284.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1525..2559)
                     /locus_tag="ACZPCL_11855"
     CDS             complement(1525..2559)
                     /locus_tag="ACZPCL_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIR1163196.1"
                     /translation="MIRLHNDYNRGACGKILEKLAQINGESYDGYGEDTWCEKAAKLI
                     REKCKAPEAAIHFLPGATQANFIVHAAALSPIQSVICPDSGHVYAHEAGSIENTGHKL
                     VPLPSTGGKISAEQIRKAAASYYNGGEPNYICEPKMVYISFPTEWGTIYSEQELKEIR
                     KVCDEYEMYLFVDGARLGYGLGSVKNDLTLEKLAELTDVFYIGGTKCGALFGEALVIT
                     NKKLARKFRTYMKQNGAVLAKGWLLGMQFCALLEDDTYEKMTAQADAYALEIKAAFKE
                     KGIREYVDSYTNQLFVILTEEQAEVLGKEYTFEHQQDLADGSKVVRFCTSWATTEAEK
                     DQLIRDIRAL"
     gene            complement(2600..4285)
                     /locus_tag="ACZPCL_11860"
     CDS             complement(2600..4285)
                     /locus_tag="ACZPCL_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1163197.1"
                     /translation="MEKLNIPVGVSDFAELRSNGYYYVDKSGLIREMLTSSAAKVTLI
                     TRPRRFGKTLGMSMLENFFNIRKNSEKLFENLEISGNRELCDQWMNQWPVIFFSLKDI
                     DGLDFQSAYDRLVVQLSNLCKMHVYLLQSEAIDPDDKKVFLELKAGTASEAQVGFSLN
                     TLMRMMESYYRKPVILLLDEYDVPLAKASSNGYYKEMLSVIKGMMSTALKDNPFLKFA
                     IITGCLKIAKESIFTGTNNLVSDTITDTRLNEYFGFIQKDVEQILRDAGSIDHLERVK
                     SWYDGYHFGRMDVYCPWDVMNYIRDIQRDSDTRPKSYWMNTSDNGIIRSFIDIAGSNI
                     TKKLETLLNGGYILQRIDENLTYDYLHSSEDNLWSILYLTGYLTSIRENELKEKLPDG
                     ISALMIPNAEIREIFETTVIQWFDDSAKAWDRKKMFSAVWKGDSRTVMEEMNRLLRKT
                     ISYHDYREDFYHAFLAGIFAGAGYVVESNKEHGEGRSDVVVYDPEEGRVAIFEAKYSK
                     TLETMDTDCQKALDQINEKMYAKEYEDDFDHILCYGVSFFKKRCCIKLKDAKV"
     gene            complement(4482..>4622)
                     /locus_tag="ACZPCL_11865"
     CDS             complement(4482..>4622)
                     /locus_tag="ACZPCL_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1163198.1"
                     /translation="TCHHCGSVKKNLKLSDRIYRCECGYVADRDFNAALNLKDAKTYK
                     IA"
BASE COUNT         1228 a         1138 c          774 g         1482 t
ORIGIN      
        1 aacaagcata ttcaggaaac cgaatgggat ttctatgcag gctacaccca acagctcatg
       61 cttaacaggt actcaccacc ctgaaggttc attttctggg taaattctac ggtatattca
      121 tctccgttct tcaccggctt gatatctgcc ccttcaaaca tggtattggt tccagtcagt
      181 tctgttccct ttttatcttt gatggtatag gtaaaaatag gagcctggat gtccgcatgg
      241 aaacggatgc actcttttat ggtgaacatt tcccccttta acagcaggtt ggtcacattt
      301 cctctggcat ctaaaagacc cagatcatag atctccgctc ttccatctcc atattcggtg
      361 cggtcagtgt taatagccag ctggtcacgc ataaggcctg tatgggataa attcattccg
      421 ccggaaaaat cggacatttc tgtaccgtcg gtatttctgt ctcccgtttc tgcactgctt
      481 tttccatctg ggagttccgc tgtttttccc tcttctgctg ccttttcggc cgccagttct
      541 gcacgcaggt catctaactt tccagccagt atcttcttat acatatctac cacttttcca
      601 gccggacctt ctgcaataaa ttcaccctta tgcagcagta caactttatc gcagtacttg
      661 atgatacttc ccatatcgtg ggtaaccatc aggatggtgg tgcccttttt gcggatctct
      721 tccattctcc ggtaacattt ggactggaag aatacgtcac ctacagaaag cgcctcatct
      781 acgatcagga tctccggatc tacgttgatg gccagcgcaa aagccagacg cacaaacata
      841 ccgctggaat aagtctttac aggctggtat acgaaatctc cgatctctgc aaaatccagg
      901 atatcctgca gctttgcatc catctctttt ttcgtatatc ccatcatggt accgttcatg
      961 tatacatttt caattcctgt ataatccatg ttgaaaccgg cacccagttc cagaagggct
     1021 gatatttttc cgtctacatc cacctgaccg gaagtaggtg taagcacgcc ggtaatgatc
     1081 tttaaaatgg tagacttgcc agaaccattc gtaccgataa tacctacggt ctctcctttt
     1141 ttcacctgga aagacagtcc tttcaaagca taaaaatcac ggtgataatt tttatgggaa
     1201 atacttacag attccttcag ccggtcgatg ggcttgtcat aaaggcggta aaccttagtc
     1261 acatccctga cactgatggc attttccttt ccctgttcat tcattgacat atgtttctcc
     1321 atctctatcg catatttttc cactttaggg tttagtatag cataaaatag ctttgtttca
     1381 tactatttct gagcagatag acacatttta catgagaaaa tacaataata acgtgaaaat
     1441 gggaaccctg taaaatcatc tttttacaga attcccatct tttatattac ttactttcta
     1501 ttgcttattt taggggtgta aatattataa tgcccggatg tcccggatca gctgatcttt
     1561 ctctgcttcg gttgtggccc agctggtaca gaaacggact accttacttc catcagccag
     1621 atcctgctgg tgttcaaagg tatattcttt tcccagtacc tctgcctgtt cttctgtcag
     1681 gatcacaaac aactggttgg tatagctgtc cacatattcc cggatccctt tttctttaaa
     1741 agcggctttg atctccaaag cataggcatc tgcctgggct gtcatttttt cgtaagtatc
     1801 atcctctaac agggcacaaa actgcatgcc aagaagccat ccttttgcca gaacggctcc
     1861 gttctgtttc atataagtac ggaacttccg cgccagcttc ttgtttgtga tcaccagtgc
     1921 ctcgccaaat aaagcaccgc acttggtacc gccaatgtaa aatacatccg tcagctctgc
     1981 cagcttttcc agtgtcagat cattttttac ggaaccaagt ccatatccca gtctggctcc
     2041 gtctacaaac aggtacattt catactcatc gcagactttg cggatctctt ttaattcctg
     2101 ctctgaataa atggttcccc actctgtagg gaatgaaata tacaccatct tgggttcgca
     2161 gatataattt ggttccccgc cattataata agaggctgct gctttacgga tctgctctgc
     2221 gctgatcttt ccgcctgtgg acggaagtgg caccagctta tggccggtat tttcaatgga
     2281 accggcttca tgtgcataca catggccgct gtccgggcag atcacgctct gaatcggact
     2341 taaagctgct gcatgaacaa taaagttagc ctgggtagcc cccggtaaaa agtgtattgc
     2401 agcttccggt gccttacatt tttcccggat cagcttagcc gccttctcac accatgtatc
     2461 ttccccatat ccatcatagc tttccccatt gatctgcgcc agtttctcca agatcttacc
     2521 gcatgcgccc cggttgtaat cgttgtgtaa acggatcata tttgattttt ctcctttgct
     2581 aatttaatgt actctttttt caaactttag catctttcag ttttatacag cagcgtttct
     2641 taaaaaagga aacaccatag cacaaaatgt gatcaaaatc atcttcatat tcttttgcat
     2701 acattttttc attgatctga tctaatgctt tttgacagtc tgtatccata gtttccagcg
     2761 tttttgaata tttagcctca aaaatagcca cacggccttc ctcaggatca taaaccacca
     2821 catcactgcg gccttctcca tgttctttat tggattctac cacataaccc gcccctgcaa
     2881 aaatacctgc aagaaaagca tgataaaagt cttccctgta atcatgatag ctgatggttt
     2941 ttcgcaaaag ccggttcatt tcttccatta ctgtcctgct gtcacccttc cacactgccg
     3001 aaaacatttt tttcctgtcc catgcttttg cgctgtcatc aaaccattgg atcactgttg
     3061 tttcaaatat ttcccgtatt tctgcatttg gtatcataag tgcagaaatc ccatctggaa
     3121 gtttctcttt caattcattt tcccgtatac tggtaagata accggtaaga taaaggatgc
     3181 tccacagatt gtcctctgat gaatgaagat aatcataagt cagattctca tctatacgct
     3241 gtaaaatata cccaccattc aataatgttt ccagtttttt tgtaatatta ctgcctgcaa
     3301 tatcgataaa ggaacgaata attccattat cacttgtgtt catccaataa ctttttggcc
     3361 tcgtatctga atctctctgt atatccctga tataattcat tacatcccat ggacaatata
     3421 catccatccg cccaaaatga taaccatcat accagctttt tacacgttcc agatgatcta
     3481 tgctcccggc atccctcagg atctgctcta catctttttg aataaatcca aaatattcat
     3541 ttaaccgggt atctgtgatc gtatcagaaa ccagattatt ggttccagta aaaatgcttt
     3601 ccttggcaat tttaagacag cccgtaataa tcgcaaattt taaaaatgga ttgtccttca
     3661 acgctgtact catcattcct ttgatcacag acagcatttc tttataatac ccgttactgc
     3721 ttgcttttgc cagtggaaca tcgtattcat ccagtaaaag aataactggc ttccgataat
     3781 aagactccat catacgcatt aatgtattta aagaaaaacc gacctgtgct tcactggccg
     3841 ttcctgcttt caattccaga aatactttct tatcatccgg atcgattgcc tcactctgta
     3901 aaagatatac atgcatctta caaagattag aaagctggac caccagacga tcatatgcgc
     3961 tttgaaaatc cagaccatct atatccttta aagaaaaaaa tataactggc cactgattca
     4021 tccattgatc acacaattct ctgtttcctg aaatttccag attctcaaat agtttttcac
     4081 tgtttttccg aatattaaaa aaattttcca gcatactcat accaagggtt tttccaaacc
     4141 gtcttggtcg cgtgataagc gtcactttag ctgccgatga agtcaacatt tcacggatca
     4201 gacccgattt atctacatag taatatccat tgctgcgaag ttctgcaaaa tccgaaactc
     4261 ctactggaat atttaacttt tccatagatc cagctgccac tcaaaatgtg tttaaatata
     4321 tccaaacaca tcgagaaatt gaagttctca cgaatacttc ttactgcttc attgtgtatg
     4381 ctttggcgat tacaagtaat acgctactca caggttcttg tacactccac agtcgttaat
     4441 tcccgaaata gccttcggta catacttaca tttgctttta attatgcaat cttgtaagtt
     4501 ttagcatctt tcaaatttag tgctgcattg aaatccctat ctgcaacata gccacattca
     4561 catcgataaa ttctatctga aagctttaaa ttttttttga cagaaccaca atgatggcaa
     4621 gt
//
LOCUS       DBMDUA010000204         4556 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7038_length_4556_cov_2.384287, whole genome shotgun sequence.
ACCESSION   DBMDUA010000204 DBMDUA010000000
VERSION     DBMDUA010000204.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7038_length_4556_cov_2.384287"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            324..1274
                     /locus_tag="ACZPCL_11870"
     CDS             324..1274
                     /locus_tag="ACZPCL_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1163199.1"
                     /translation="MNTQQQNSRLTSLPVVMFLAGICCFLWGSATPTIKTGYRLFQIQ
                     SSDAMSIILFAGMRFLLAGFLVILFHSIQGRRWIMPQKGSGRAILELCLNQTVLQYYF
                     FYTGLAHASGVHGAIITGGNVFISILVASLIFRYEKLTARKLVGCALGFVGIVVMNLS
                     GQEGNLFQVSLLGEGFVLFAQIFYATSGALLKKYGNQYDVVTLSGYQFMLGGLILILI
                     GLFGGGRVQVESGVSAYILLLYLAFISAVAYTLWGVLLKYNPVSRVTVFGFMNPVFGV
                     LLSALVLGETAQAFSVKSLVALVFVCLGIYVVNSVKTAER"
     gene            1414..2499
                     /locus_tag="ACZPCL_11875"
     CDS             1414..2499
                     /locus_tag="ACZPCL_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR1163200.1"
                     /translation="MSQQKRDYYEVLGIDKTADPATIKKAYRKLAKKYHPDSNVGNEA
                     AAEKFKEVNEAYDILSDPEKKKLYDQFGHAAFDGTGAAGAGSYGANGGFGSDGSFTGG
                     FGGFSGNGGFRGFHTSDGHTFHFEGGEGDDIFGDIFGNMFGGGKKSRFTGGFHQDGSG
                     FNGFHGYSGFGADPSSEKGSDAQAEINISFDDAAFGADRVITLSDANGKSQNLKVHIP
                     AGINEGQSIRLKGKGNPGIGGAEAGDLLLKVHVGTRPGYERKGMDVYTSTEVPFTTAV
                     LGGEIIVPTLYGNVSCKLREGTRCGLKIRLAGKGIVSMKDPKVKGDQYVTIQIAVPQN
                     LTAEAKEKLREFAAACEKGRRGGRTAA"
     gene            2538..>4556
                     /locus_tag="ACZPCL_11880"
     CDS             2538..>4556
                     /locus_tag="ACZPCL_11880"
                     /inference="COORDINATES: protein motif:HMM:NF014026.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR1163201.1"
                     /translation="MKKNRNPERTKTLKEKGKIPFRYRFFLGGASFFLALMILVTGVA
                     FAFRETIDLYLSGSRTNVSKEAKEEAAYNSRRLAAQIEAEGAVLLKNENNTLPLSEEI
                     TRVNVFGWASTQWLGSGSGSGRVEKVDLDLLGALKEAGISYNEELTRMYQDFQPKRQK
                     TSTLESWPEESCRLYEPDITDTEYYTEDLLKNAKEYSDTAIMVVGRLSGESNDCPQIQ
                     YKKVKKNGQLLRDKRRSYLDLSTEEEGLLRYLGENYKNVILLLNTGNVMTLGAIDLMP
                     GIGACVMAGMTGQYSAEALPDLLWGKITPSGRTADTWAYNFRTAASYANAGADGVGSY
                     ENGEGLYPFDGTKSGNVGESFKYDQVSYVDYAEGIYVGYKWYETADAQDYWDKYYNLH
                     GRGYQAVVQYPFGYGLSYTTFDWKVVNAPGKREKITADGSYKITVEVTNTGERAGKEV
                     VQLYYAPPYEAGGIEKSAVVLADYAKTGELQPGEKEEVTLSVKARDMASYDFNDSNQN
                     GFAGYELDPGTYVLSLRKNAHETADIPNAQISLKLAENIQYPTDEITGAVVSNKMTGK
                     DAVDGVGIDGSDSGQKISYMSRSDFDTTFPKEPVKTRKLSEKAAELNLYTQEKADEFG
                     QSFLEQSESQSKNDVAFGKKQGLYIEKNGTVTKLGYELGADYDDPKWDE"
BASE COUNT         1401 a          873 c         1125 g         1157 t
ORIGIN      
        1 cacgaataca agaggcaatt ccttaacgct tacagactga ttgaaacgac ataataaatt
       61 ctcctgttct ttcacacata ttttattttc cccaggccat gtgatctata aaatccccag
      121 taaaacatat aaatagacaa acatggcaaa acagtacaaa atatttttat acacctaaaa
      181 tattttgtgt atagtgtaaa acaaaattga gaaaatttca atacttattc acaacttatt
      241 gataaaaaat tgactttcaa tctgtttact ggtactctta taaggataaa cgagctacag
      301 acagtagcag aatggagaag aatatgaata cacagcaaca aaacagcaga ctgaccagtc
      361 tccccgtagt aatgtttttg gctggtatct gctgtttctt atggggaagc gcgacaccga
      421 cgatcaaaac aggttacaga ctgttccaga tccagtcatc tgacgcaatg tccattattt
      481 tatttgcagg aatgcgtttc ctattggcag gttttctggt aatccttttt cacagcatcc
      541 agggacgccg ctggatcatg cctcagaagg gatcaggaag agcgatcctg gaactttgct
      601 taaatcagac ggtccttcag tattatttct tttacacagg actggcacat gccagcgggg
      661 tacatggagc gatcatcaca ggaggaaatg tttttatttc tattttagtt gccagcctga
      721 tcttccggta tgagaaactg acagccagaa aactggtggg atgcgccctg ggttttgtgg
      781 gaattgtagt aatgaacctg tccggacagg aaggaaatct gttccaggta tctcttttag
      841 gagaaggatt tgtcctgttt gcccagatct tttatgctac atccggcgcc ttattaaaga
      901 aatatggcaa ccagtatgat gtagttacct taagcggtta tcagtttatg ttaggcggac
      961 tgatcctgat cctcattgga ttattcggcg gcggaagagt ccaggttgaa agcggagttt
     1021 ccgcgtatat cctgttatta tatctggcat ttatctcagc agttgcttat acactctggg
     1081 gtgtattgtt aaaatataac cctgtaagcc gcgtgaccgt atttggcttt atgaacccag
     1141 tgttcggtgt tcttttgtcc gccctggtat taggggagac agcccaggca ttttccgtaa
     1201 agagcctggt tgccctggta tttgtatgcc ttggaattta tgtggtcaac agtgtaaaaa
     1261 cagcagaacg ataaagaaaa tgtatgttcg ttccatcctt gacaatgaat gatataagtg
     1321 ttatacatat tatagaacaa acaaatataa cttgtattta taacttagag gattttcaca
     1381 ggtcctgtaa gaaatcagga aaggatgaga acgatgtcac aacagaaacg agattactac
     1441 gaagttcttg gaatagataa aactgcagac cctgccacta ttaagaaagc ctatcgtaaa
     1501 ctggctaaaa aataccatcc cgacagcaat gtgggaaatg aggcagctgc tgaaaaattt
     1561 aaggaagtta atgaagctta tgatatttta agcgatccgg aaaagaagaa actgtatgac
     1621 cagtttggcc acgcagcttt tgacggtacc ggagcagcag gagccggtag ttacggcgca
     1681 aatggcggct ttggttctga cggcagcttt acaggaggtt ttggcggctt ttccggaaat
     1741 ggcggcttca gaggtttcca tacttcagac ggacacacct tccattttga aggcggagaa
     1801 ggcgatgata tctttggtga tattttcgga aatatgttcg gcggcggtaa aaagagccgc
     1861 tttacaggcg gttttcacca ggacggcagt ggctttaacg gttttcatgg ctatagcggt
     1921 tttggcgcag atccatccag cgaaaaaggc agtgatgcac aggcggagat caatatcagt
     1981 tttgatgatg cagcttttgg cgcagaccgt gtgatcacac tttccgatgc aaatggaaag
     2041 agccaaaatc tgaaagtcca tatccctgca ggtatcaacg aaggccagag catccgcctg
     2101 aaaggaaaag gcaatcctgg cataggcggc gcagaagcag gagatctgtt attaaaggtc
     2161 catgtaggaa cacggcctgg ttatgaacgg aagggaatgg atgtttatac cagtacagaa
     2221 gtacccttta caacagctgt tttaggcggt gagatcattg tacctaccct gtatggtaat
     2281 gtttcctgca aactgcgtga gggaaccaga tgcggcttaa agatccgtct tgccggtaaa
     2341 ggtattgttt ccatgaaaga cccgaaggta aaaggcgacc agtatgtgac catccagatc
     2401 gctgttccgc agaacctgac agcagaggca aaagaaaaac tgagagaatt tgcagctgcc
     2461 tgtgaaaagg gcagaagagg cgggagaaca gccgcttaat gatctataat attaaaaata
     2521 ataatgtgag gtaccttatg aaaaagaacc gaaatccaga aagaacaaaa accttgaaag
     2581 aaaaaggtaa gatccctttc cggtaccgct tctttttggg aggtgcctct ttttttcttg
     2641 cccttatgat ccttgttaca ggtgtggctt ttgcgtttcg tgagaccatt gacctgtatc
     2701 tttccggcag ccgtaccaat gtatcaaaag aggcgaaaga agaagctgcc tataacagcc
     2761 gccgtctggc agcccagatc gaggcggaag gtgctgttct tttgaaaaat gaaaacaata
     2821 ccctgcctct ttcagaagag atcacccggg taaatgtatt tggctgggca tccacccagt
     2881 ggctgggaag cggttccggt tccggaagag tggaaaaagt agacctggat cttttaggag
     2941 cattaaaaga agcgggaatc tcctataatg aagaactgac ccggatgtat caggatttcc
     3001 agccaaagcg tcagaaaaca tctactcttg aaagctggcc ggaagaaagc tgccggcttt
     3061 atgaaccgga tattacagat acagaatatt acacagaaga tctgcttaaa aacgcaaaag
     3121 agtattcaga tacagcgatc atggtagtag gaaggctttc aggggaaagc aatgactgtc
     3181 ctcagatcca gtataaaaaa gtaaagaaga acggacagct gttaagagat aaaagaaggt
     3241 cctatctgga tctttccaca gaagaagagg ggcttttgcg ctatctgggt gaaaattata
     3301 aaaatgtgat cctgttatta aatacaggaa atgtaatgac cttgggagca attgacctga
     3361 tgcctggaat tggggcctgt gtgatggcag gaatgaccgg acagtattcg gcagaggcac
     3421 ttccagacct tctctgggga aaaataacac cttccggccg cacggctgat acctgggctt
     3481 acaatttccg cacagctgcc agctatgcca atgcaggagc tgacggagtg ggaagttatg
     3541 aaaacggaga aggtctttat ccttttgatg gtaccaaaag cggcaatgta ggagagtcct
     3601 ttaaatatga tcaggtatct tatgtagact acgcagaggg gatttatgta ggctataaat
     3661 ggtatgaaac agcagatgcg caggattact gggataagta ttataatctt catggaagag
     3721 gttaccaggc tgtagtacag tatccttttg gctatggctt gtcttatact acctttgact
     3781 ggaaggttgt aaatgctccg ggaaaaaggg aaaagatcac tgcggacggc agttacaaga
     3841 tcacagtgga agttaccaat acaggagaac gggctggcaa agaggtggtg cagctttatt
     3901 atgcccctcc atatgaagcg ggagggatcg aaaaatcggc ggtagtatta gctgattatg
     3961 cgaaaacagg agagctgcag ccgggggaaa aagaagaggt tacgttgtct gtaaaagcca
     4021 gagatatggc atcctatgat tttaatgaca gcaatcaaaa cggttttgca ggatatgagc
     4081 tggatccggg aacctatgta ctttctcttc ggaaaaacgc ccatgagacc gctgatatac
     4141 caaatgccca gatcagcctg aaactggcag aaaatatcca gtatcctaca gacgagatca
     4201 caggtgcagt cgtttccaat aaaatgacag gaaaagacgc agtagacgga gtggggatcg
     4261 atggttcaga cagcggtcag aagatctcct atatgagccg cagtgatttt gatacgacat
     4321 ttccaaaaga accggtaaag acccgaaaac tttctgaaaa ggcagcagaa ctgaaccttt
     4381 atacccagga aaaagcagat gagtttgggc agagcttttt agaacaaagt gagtctcaga
     4441 gtaaaaatga tgtggcattt ggaaaaaagc agggccttta tattgaaaaa aacggaactg
     4501 taacaaaatt gggttacgaa ctgggagcgg attatgacga tccaaagtgg gatgaa
//
LOCUS       DBMDUA010000205         4540 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7067_length_4540_cov_2.359315, whole genome shotgun sequence.
ACCESSION   DBMDUA010000205 DBMDUA010000000
VERSION     DBMDUA010000205.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7067_length_4540_cov_2.359315"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..4340
                     /locus_tag="ACZPCL_11885"
     CDS             <1..4340
                     /locus_tag="ACZPCL_11885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HIR1163202.1"
                     /translation="LQMKLSRTTKAQIVFEDEGKMVLTVEDTMVNRDKTNELKRVLEK
                     VFNERCGLPVEVAFQYIPPKENDRRKQIELKLEREAQAIYWQNHKDELENVSGGENIS
                     QTVAKSDNGEKKTHNSNAAIMDIPMPEAAPWDALMDQAASGNVPGNDETISPVSDGIK
                     PVDAGKTAAAKPQSGGKTEKPQLNKGRKGWGRDKDSGFGEDRKFSVKRSNNPDVLYGR
                     DFEDDFMEIEKIDGEIGEVTLRGQILTCESRELRSGKFILTFDVTDFTDTITAKMFIR
                     PEIFNEVKDMIKTGMFIKIKGVTTIDKFDGELTLGSIVGIKKADDFTTKRMDNSLEKR
                     VELHCHTKMSDMDGVSEVKSIIKRAKKWGMSSIAVTDHGCVQAFPDANHALDKGDTFK
                     ILYGVEGYLVDDTKQLVENSKGQTFADTYVVFDIETTGFSPVKNRIIEIGAVKVVDGV
                     ITDKFSTFVNPDVPIPFDIEKLTSINDAMVLPYPKIDVILPQFLEFVGDAVLVAHNAG
                     FDVGFIGHYAEALGLPFSPTVLDTVSLARLLLPNLNRFKLDTVAKALNISLENHHRAV
                     DDAGATAEIFAAFVKMLRDRDIEDLDHLNELSTMDAQTIMKLPTNHVIILAKNDLGRI
                     NLYRLVSWSHLDYYSRRPRIPKSVLDKYREGLLIGSACEAGELYQALLRGAPDAEIAR
                     LVNFYDYLEIQPLGNNAFMLRDEKSTVKTEEDLIEINKKIVDLGTQFGKLVCATCDVH
                     FLDPQDEVYRRIIMAGQGFKDSDDQAPLYLRTTEEMLEEFAYLGPDKAYEVVVTNTRK
                     ISDMCEKIAPVRPDKCPPVIPDSDKTLTKICYDRAHEMYGEDLPKIVVDRLERELHSI
                     ITNGFAVMYIIAQKLVWKSNEDGYLVGSRGSVGSSFVATMSGITEVNPLSPHYHCPKC
                     RYYDFDSEEVRKFSGMAGCDMPDKKCPVCGTPLEKDGFDIPFETFLGFKGDKEPDIDL
                     NFSGEYQSKAHDYTEVIFGKGQTFRAGTIGTLADKTAFGYVKKYYEERGTRKRNCEIN
                     RIVQGCVGVRRTTGQHPGGIIVLPLGEEIYSFTPVQHPANDMTTSTVTTHFDYHSIDH
                     NLLKLDILGHDDPTMIRMLQDLIGLDPVKDIPLDSPEVMSLFQNTSALGITPDDIGGC
                     PLGALGVPEFGTDFAMQMLLDTKPKYFSDLVRIAGLAHGTDVWLGNAQTLIQEGKATI
                     QTAICTRDDIMIYLIAQGMEQGLSFTIMESVRKGKGLKPEWEEDMKAHGVPDWYIWSC
                     KKIKYMFPKAHAAAYVMMAWRIAYCKIFYPLAYYAAFFSIRASAFSYEIMCQGRDKLE
                     YYLADYKKRSDTLSKKEQDTLRDMRIVQEMYARGFDFMPIDIYKAKARHFQIIDGKLM
                     PSLSSIDGLGEKAAEAVVDAVKDGPFLSVEDFSSRTKVNGTICSLMSDLGLLKDLPQS
                     NQLNLFELM"
BASE COUNT         1308 a          904 c         1142 g         1186 t
ORIGIN      
        1 atctccaaat gaaattatct cgaactacca aagcccagat cgtctttgaa gatgaaggaa
       61 aaatggtgct gactgtagaa gataccatgg tcaaccggga caagaccaat gagttaaagc
      121 gggttttgga aaaggtattt aatgaacggt gcggtctgcc ggtagaagta gcgtttcagt
      181 acattccgcc aaaggaaaat gaccgtagaa agcagatcga gctgaagtta gagagagaag
      241 cacaggctat ttactggcaa aatcataagg atgagttaga aaatgtaagt ggtggagaaa
      301 atatctcgca gacagttgca aagagcgata atggtgagaa aaaaactcat aatagtaatg
      361 ctgctattat ggatattcct atgccggagg cagctccatg ggacgctctt atggatcagg
      421 cagcttccgg aaatgttcct ggcaatgatg agactatttc tcctgtatca gatggcataa
      481 aaccagtaga tgcaggaaaa acagctgctg caaaaccaca gtcaggcgga aaaacggaaa
      541 aacctcagct taacaaaggc agaaaaggct ggggaagaga taaagactcc ggctttggag
      601 aagacaggaa gttttctgtt aaacgttcca ataatccgga tgtactttac ggccgtgatt
      661 ttgaagatga ctttatggag attgagaaga tcgacggaga gatcggagaa gttactttac
      721 gtggacagat cttaacctgt gaaagccgcg agctaagaag cggtaaattc attcttacct
      781 ttgatgtgac agactttaca gataccatta cagctaaaat gttcatccgt ccggaaatct
      841 tcaatgaagt aaaggacatg atcaaaacgg gcatgttcat taagatcaaa ggtgttacca
      901 ccattgataa gtttgacgga gaactgacat taggctctat tgtaggtatt aaaaaggccg
      961 atgactttac caccaagcgt atggacaaca gcctggaaaa acgtgtggaa ctgcactgcc
     1021 atacaaaaat gagcgatatg gatggtgttt ctgaggtaaa gagcatcatc aagcgggcga
     1081 aaaaatgggg tatgtcctcc attgctgtta cggaccacgg ctgcgttcag gccttccctg
     1141 atgcaaacca tgcattggat aaaggggata cctttaagat cttgtatggt gtggaaggct
     1201 atctggtaga tgataccaag cagctggtag aaaattccaa gggacagact tttgcagata
     1261 cctatgttgt tttcgatatt gagaccacag gtttcagccc tgtaaagaac cggatcattg
     1321 agatcggtgc tgtaaaagta gtagacggtg tgatcacgga caagttttct actttcgtaa
     1381 acccggatgt gccgattcca tttgatattg aaaagctgac cagcatcaat gacgctatgg
     1441 tactgccata tccgaagatc gatgtgatat tacctcagtt tctggaattt gtgggagacg
     1501 cagtactggt agcccacaat gcaggttttg atgttggttt tattggtcat tatgcagaag
     1561 cgctgggact gccattttca ccaacagtac tggatacggt atctctggcc cgccttcttc
     1621 tgccaaactt aaaccgtttt aagctggata cagtagcgaa agctttaaat atttccctgg
     1681 aaaatcatca ccgggcagta gatgatgcag gagctacagc ggagattttc gcagcttttg
     1741 taaagatgct gcgggatcgg gatattgaag atctggacca cttaaatgaa ttaagtacca
     1801 tggatgccca gaccattatg aaactgccta ccaaccatgt gatcatcctg gcaaaaaatg
     1861 acctgggacg tatcaacctg taccgtctgg tcagctggtc ccatttggat tattattcca
     1921 gacgtccccg tatcccaaag agcgttctgg acaagtacag agaaggtctg ctgatcggtt
     1981 ctgcctgcga ggcgggagaa ttatatcagg cccttcttcg cggggctccc gatgcggaga
     2041 ttgccagact ggtaaatttc tacgattatc tggagatcca gcctttggga aacaatgcgt
     2101 ttatgctccg ggatgaaaaa agtacagtta agacagaaga agacttgatc gagatcaata
     2161 aaaagatcgt agatctggga acccagtttg gtaagctggt gtgcgctacc tgtgacgttc
     2221 atttcctgga tccccaggat gaggtttacc ggcgtatcat catggctggt cagggattta
     2281 aagactccga tgaccaggcg ccgctatatc tgcgtacaac agaagagatg ttagaagagt
     2341 ttgcttatct gggaccggat aaggcatacg aggtagtcgt taccaatacc cgtaagattt
     2401 ctgatatgtg cgaaaaaatt gcgccggtgc gtccggacaa gtgcccgcct gttatcccag
     2461 actctgataa gaccttaaca aagatctgtt atgaccgcgc tcatgagatg tatggtgaag
     2521 atcttccaaa gatcgtagta gaccgtctgg agcgtgagct tcattccatt atcaccaatg
     2581 gatttgcggt tatgtatatc attgcccaga agctggtttg gaaatctaat gaagacggct
     2641 atctggtagg ttcccgaggt tctgtaggtt cctcctttgt ggcaaccatg tccggtatta
     2701 cagaggtaaa tccgctaagt cctcattatc attgtccaaa gtgccgttac tatgattttg
     2761 actctgaaga agtgcgtaag ttctccggta tggcgggctg cgatatgccg gataaaaaat
     2821 gcccggtatg cggaacacct ctggaaaaag acgggtttga tatcccattt gagactttcc
     2881 ttggatttaa gggagataag gagcctgata tcgacttaaa cttctccggt gagtaccaga
     2941 gtaaggccca tgactatacg gaggttattt tcggtaaggg ccagactttc cgtgcaggaa
     3001 ccattggtac actggcggat aaaacggctt tcggttatgt aaagaaatat tatgaggaaa
     3061 ggggaaccag gaagcgaaac tgcgagatca accgtattgt tcagggctgt gtaggcgtgc
     3121 gccgtaccac cggccagcat ccgggaggta tcattgtact gcctttaggt gaagagatct
     3181 attcctttac accggtacag cacccggcaa acgatatgac tacatccaca gttaccacac
     3241 actttgatta ccattccatt gaccacaacc tgttaaaact ggatattctt ggacacgatg
     3301 atcctaccat gatccgtatg ctgcaggacc tgatcggtct ggacccggtt aaggatattc
     3361 ctctggacag cccggaggtt atgagcctgt ttcagaatac ctctgcactg gggatcacgc
     3421 cggatgatat cggtggctgt cctttaggag ctcttggtgt gccggagttt ggtacggact
     3481 ttgctatgca gatgcttctg gataccaaac caaagtattt ctctgacctg gtgcgtattg
     3541 ccggtctggc acatggtacg gacgtttggc tgggaaatgc ccagacgttg atccaggaag
     3601 gaaaagctac tattcagaca gcgatctgta cccgagatga tattatgatc tatctgatcg
     3661 cccagggaat ggaacaggga ctttccttca ccatcatgga gagcgtgcga aaaggtaaag
     3721 gtttaaagcc ggagtgggaa gaagatatga aggcgcatgg tgtgcctgac tggtacatct
     3781 ggtcctgtaa aaagatcaag tacatgttcc caaaagcaca tgcggcagcc tatgttatga
     3841 tggcgtggcg tattgcctac tgtaagatct tttatccact ggcttactat gcggcctttt
     3901 tcagtatccg cgccagcgcc ttttcctatg agatcatgtg tcagggaaga gataaactgg
     3961 aatattatct ggcagattat aaaaaacgtt ccgatactct ttctaaaaag gagcaggata
     4021 ccctgcgtga catgcgtatt gtccaggaaa tgtacgcaag gggctttgat tttatgccaa
     4081 ttgatattta taaggcaaaa gcaagacatt tccagatcat tgacggtaag ctgatgcctt
     4141 ctttaagcag tattgacggt ttaggtgaaa aagcggcaga agcagtggta gatgcagtaa
     4201 aagacggtcc gttcctgtca gtagaagatt tctccagccg taccaaggta aatggaacta
     4261 tctgttccct gatgtcagac cttggactgc ttaaagacct tcctcagtct aatcagttaa
     4321 atttatttga gctgatgtag gagattatgt aagagattat gcatagtagt ctggtatcat
     4381 aaaattgact tataaaagaa tttatggtat atttttgaaa gagttttttc acatgtggag
     4441 tgtggaaaaa ctctttcttg cgctttaggg gcaaattgac gcaccaaagt gttaaagtgt
     4501 atgcgctgga aaaaaatgaa aacagcgtaa atcaaagtag
//
LOCUS       DBMDUA010000206         4500 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7137_length_4500_cov_2.095704, whole genome shotgun sequence.
ACCESSION   DBMDUA010000206 DBMDUA010000000
VERSION     DBMDUA010000206.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7137_length_4500_cov_2.095704"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="ACZPCL_11890"
     CDS             <1..831
                     /locus_tag="ACZPCL_11890"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HIR1163203.1"
                     /translation="QNTLGIHLFKRMNGRYTLTSAGETFCKYAEQIIELEKNMEQAMQ
                     TYKNTTILKIGTSTTKAISMVTHLIPIYRKEFPNIDIAMSEGNSFTIISKVLNNDLDL
                     VFGSISSLDLYKGQILPLKEEKIALAVPSRMSICRNSNYNYIQSLDLETFARELSGAP
                     FILQHPGSCIRYLADGLFRSAHMTPVVILNTSNASSIVKSVSSGIGAGFIPVSNMVLD
                     PNIVYFLFTPSLYRIHCMVYRKKIEKDTAFQRLSELSREYADRWTDMDPEIGDLVPYA
                     "
     gene            complement(940..3033)
                     /locus_tag="ACZPCL_11895"
     CDS             complement(940..3033)
                     /locus_tag="ACZPCL_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR1163204.1"
                     /translation="MNVYGTEQIRNVVLLGHGGAGKTTVAEAMALITGVTKRMGKVAD
                     GNTISDYDKEEIKRQFSISTTLIPLEYQGENGPVKVNLLDTPGFFDFVGEVEEAISVA
                     DAAIIVVNCKAGIEPGTERAWEMCEKYNLPRLIFVTNMDDDHASYRELVVKLEKKFGR
                     KIAPFQLPIRENEKFVGFVNVVKMAGRRFTNLSDYVECEIPDYVQKNLTIARDALIEA
                     VAETSEEYMERYFLGEEFTQEEISGALRAHVIEGDIVPVMMGSGINCQGFKVLLQVID
                     KYFPAPNHFECIGVDVSTGERFTAKYNDDVSLSARVFKTIVDPFIGKYSLMKICTGTL
                     KGDTVVYNVNKDAEEKIGKVYVMRGKEVIEVPELKAGDIGAVAKLTVTGTGDTMAVRT
                     APIVYHKPQISTPYTYMAYKAANKGEDDKVSTALARMMEEDLTLQVKNDPENRQSLIY
                     GIGDQQLDVVISKLQTRYKVDVVLSKPKFAFRETLRKKVKVQGKHKKQSGGHGQYGDV
                     IMEFEPSGDLETPYTFEEKVFGGSVPKNYFPAVEKGLQECVLKGPLAGYPVVGLKATL
                     LDGSYHPVDSSEMAFKMATILAFKQGFMDANPVLLEPIASLKVVAPDRFTGDIMGDLN
                     RRRGRVLGMESDHHGKQVIEADIPMSELFGYNTDLRSMTGGLGDYSYEFSRYEQAPGD
                     VQKREVEARAAAKEE"
     gene            complement(3228..>4500)
                     /locus_tag="ACZPCL_11900"
     CDS             complement(3228..>4500)
                     /locus_tag="ACZPCL_11900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1163205.1"
                     /translation="TELSQPLDTMMEILVGIAVLYMVLLITATVYILYNRFRTISQYR
                     EIAYLKEINQGMEMVAKKNDEIRHYQRIQSLGMMASHIAHEFNNYLTPVLIYSELLEN
                     DDTVSEENRQMIHEITDSVDKASDLSKKLLAFSRQDTGIRLERLDFAQEVRKAMSVVR
                     QLVPAAITLKTKIPDTPQYALVRQGMAEHILMNLCKNAFQAMEQSDKKELTVELAEMG
                     TDQLCLRISDTGCGIPEDVQKKIFEPFYTTKGSRQGTGLGLSVVRNIIASVGGKIEVE
                     SRQGEGTTFLLMIPKSSSEEEGNLRKRLKSVSRIAIISEDARMSAYKGKKFEKTGQIE
                     VYTHPAALLDRVQKTPSFYGLIIADEVLPMMNGIELCEMIRRMNPEIRLLLLSEQDGS
                     DVQWYLNNGMIDRFMLKQDFFSEFEETIKAK"
BASE COUNT         1157 a         1096 c          845 g         1402 t
ORIGIN      
        1 caaaataccc tgggaatcca cctgtttaaa cgaatgaacg gccgttatac ccttacttct
       61 gccggtgaga cattctgcaa atatgcagaa caaatcattg aactggaaaa aaacatggag
      121 caggccatgc aaacctataa aaatactact attttaaaaa taggtacatc taccacaaaa
      181 gctatctcca tggtgaccca tcttattccc atataccgca aagaattccc aaatattgac
      241 attgctatgt ccgaaggcaa ttcctttacc attatttcaa aagtgttaaa caatgatctg
      301 gaccttgtct ttggctccat ttcttccctg gatctttata aaggccagat cctgccactg
      361 aaagaagaaa agattgctct ggctgtccca agccggatgt ctatttgccg caactcaaac
      421 tataactata ttcagagtct ggatctggaa acttttgcaa gagagctttc cggtgctcct
      481 tttatccttc agcatccagg ttcctgcatc cgttatctgg ctgacggcct gttccgttct
      541 gcccatatga cccctgtagt tatcctgaat accagcaatg catcttctat tgtaaaatct
      601 gtttcctctg gtataggtgc aggatttatc cctgtaagca atatggtatt ggaccccaat
      661 atcgtatatt ttttgtttac accttctctt taccgcatcc attgtatggt ctatcgaaaa
      721 aagatagaaa aagatactgc atttcaacgt ctttctgaac tgtcccggga atacgcagat
      781 cgctggaccg atatggaccc ggaaatcgga gatctggttc cttatgcctg attttcttac
      841 ttaacaacac aaaaatagcc ctaaaatcaa taacagagcg ggacctttca aagatcccgc
      901 ccttattgtt tatagagctt tttatttctg ttttctcaat tattcttcct tcgcagcagc
      961 cctagcttct acttcgcgct tctgtacatc gccaggagcc tgctcgtagc ggctgaattc
     1021 ataggaatag tctcctaaac caccggtcat ggaacgaaga tcggtgttgt agccgaacaa
     1081 ttcagacatt ggaatgtctg cttcgatgac ctgtttgccg tggtggtcag attccatacc
     1141 taatacacgg cctcttctgc ggtttaagtc acccatgata tcgccggtga agcggtcagg
     1201 agcaacaacc tttaaggaag caatcggctc taaaagcact gggtttgcat ccataaagcc
     1261 ctgtttaaat gccagaatag tagccatctt aaatgccatt tctgaagagt ctaccggatg
     1321 ataagaacca tctaacagag tagcctttaa gccaacaacc ggatatccag ccaacggtcc
     1381 cttaagaacg cattcctgca gtcctttttc tactgccggg aagtagttct tcggtacgga
     1441 tccaccgaat accttctctt caaaggtgta tggtgtctct aaatcgccgg atggctcaaa
     1501 ttccatgata acatcaccgt actggccatg tccgccggac tgtttcttat gtttgccctg
     1561 tacttttact ttctttctta aggtctcacg gaaagcaaat ttcggtttgg acagaactac
     1621 gtctactttg tagcgggtct gcaacttgct gatcacaaca tctaactgct gatcgccgat
     1681 accatagatc agggactgac ggttttcagg atcattctta acctgtaagg tcaggtcttc
     1741 ttccatcata cgtgctaatg cggtagaaac cttatcatct tcgcccttat tagctgcctt
     1801 gtatgccata tatgtatatg gtgtggagat ctgtggtttg tggtaaacga tcggagctgt
     1861 acgaactgcc atggtatcgc cggttccggt tacagtcagt tttgcaactg cgccgatatc
     1921 gccggccttt aactcaggaa cttcgataac ttccttgccg cgcattacat aaactttacc
     1981 gatcttctct tctgcatctt tatttacatt gtatacaacc gtatcgccct ttaaggtacc
     2041 agtacagatc ttcatcaggg aatactttcc gataaatgga tctacgatgg tcttaaatac
     2101 tcttgcagaa agggatacgt catcattata tttagcggtg aaacgctcgc ctgtggaaac
     2161 gtctacaccg atacactcaa agtgatttgg tgccgggaag tatttgtcaa taacctgaag
     2221 gagaacctta aagccctggc agttgattcc ggagcccatc ataacaggta cgatgtcgcc
     2281 ttcgataaca tgtgctctta atgcacctga gatctcttcc tgtgtaaatt cttctcccag
     2341 gaaataacgt tccatatatt cttcactggt ttctgcaact gcctcgatga gggcgtctct
     2401 tgcgatggtt aagttcttct gaacataatc tggaatctcg cactcaacat aatcactgag
     2461 gtttgtaaag cggcggcctg ccatctttac tacgtttacg aaacctacga atttttcatt
     2521 ttcacggatt ggaagctgga aaggagcgat ctttcttccg aactttttct ccagctttac
     2581 aaccagctca cgatagctgg cgtgatcatc gtccatatta gtaacgaaga tcagtcttgg
     2641 cagattgtac ttttcgcaca tctcccatgc tctttctgta ccaggctcga tacctgcttt
     2701 acagtttaca acgataatgg ctgcatcagc aacgctgatg gcttcttcta cttcacctac
     2761 gaagtcaaaa aatccgggag tatccagtaa gttgacctta accggtccgt tctctccctg
     2821 gtattccaga ggaatcagtg tggtggaaat ggaaaactgt ctcttgatct cttctttgtc
     2881 ataatcacta atagtgtttc cgtcagcaac tttgcccata cgcttggtaa cacctgtgat
     2941 caaagccatt gcctccgcaa ctgttgtctt accagcgcct ccatgcccaa gtaatacaac
     3001 gttgcggatt tgttcagtac catatacgtt cataaacttc cctcctgtac actttatttg
     3061 tgcgaaacaa ctgtaatatg tgcttcgtct ttgccatgtg tacattctac tatatttttc
     3121 tgaaatattc aagttttttt gttaaattct acacaacatt tttccaaaac attccagata
     3181 ttttgattta agtcaccaca tgttcctgtt tttctcatct ttaaccgtca ttttgcctta
     3241 attgtctctt caaattcgct aaaaaagtcc tgtttcaaca taaaacggtc gatcatgcca
     3301 ttattcagat accactgaac atcacttccg tcctgttctg ataacaataa aagacggatc
     3361 tccggattca tccggcggat catttcacat aactcgattc cattcatcat gggaagaact
     3421 tcatctgcaa tgatcaggcc ataaaaagaa ggcgttttct gtacccggtc aaggagggct
     3481 gccggatggg tataaacttc gatctgccca gtcttttcaa attttttccc tttataagca
     3541 gacatcctgg catcttctga tataatagcg atcctggata cagattttaa acgttttcta
     3601 agattgcctt cctcttctga actacttttg gggatcataa gcaaaaaggt cgttccttct
     3661 ccctgacggc tttctacctc aatctttccc cctacagacg caatgatatt tcttaccacg
     3721 gaaagcccca gtcccgttcc ctgtctggaa ccttttgttg tataaaatgg ctcgaagatc
     3781 ttcttttgca catcttccgg gatcccgcat cctgtatcag aaatacgcag gcaaagctgg
     3841 tctgttccca tttcagctaa ttccactgtc agttcttttt tatcagactg ttccattgcc
     3901 tgaaatgcat ttttgcacag attcatcagg atatgttctg ccattccttg tcttacaaga
     3961 gcatactgag gtgtatcagg aatttttgtt tttaatgtaa tggctgccgg aaccaattgt
     4021 cgtactactg acatggcctt tcttacttcc tgtgcaaaat ccagccgttc taaccggatc
     4081 cctgtatcct gtcttgaaaa tgccagcagc ttttttgaca gatctgatgc cttatctacg
     4141 gaatctgtga tctcatggat catctgccgg ttttcttctg atacggtatc atcgttctcc
     4201 agaagttccg aatagatcag gaccggggtc agataattat taaattcatg tgctatatgg
     4261 cttgccatca tgccaagaga ctgtattctc tggtaatggc ggatctcatc atttttcttt
     4321 gccaccatct ccattccctg gtttatttcc ttaaggtacg ctatttccct gtactgtgat
     4381 atggtacgga accggttata aagaatgtat accgttgccg tgatcagcag caccatatat
     4441 aaaacagcga tccccaccag gatctccatc atagtatcta aaggttggga aagctctgtt
//
LOCUS       DBMDUA010000207         4496 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7151_length_4496_cov_2.602958, whole genome shotgun sequence.
ACCESSION   DBMDUA010000207 DBMDUA010000000
VERSION     DBMDUA010000207.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7151_length_4496_cov_2.602958"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(236..1933)
                     /locus_tag="ACZPCL_11905"
     CDS             complement(236..1933)
                     /locus_tag="ACZPCL_11905"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1163206.1"
                     /translation="MDVTKFEELQLDDRIIRAITEMGFEEASPIQAQAIPVVLEGRDM
                     IGQAQTGTGKTAAFGLPLLQKVDPKVKKLQAIVLLPTRELAIQVAEELRRFAKFMHGI
                     KVLPVYGGQDIVRQIRALKDGTQIVVGTPGRVMDHMRRKTVKMDHVHTVVLDEADEML
                     NMGFLEDMETILSQLPEERQTLMFSATMPQAIADIAKKFQKDPVTVRVIKKELTVPKV
                     TQYYYEVKPKNKVEVMCRLLDMYSPKLSIVFCNTKRQVDELVQALQGRGYFAEGLHGD
                     LKQVQRDRVMESFRNGRTDILIATDVAARGIDVGNVEAVFNYDIPQDDEYYVHRIGRT
                     GRAGREGKAFSLVVGKEVYKLRDIQRYCKTKIIPQAIPSLNDITEIKADKILDQVQDI
                     LNDADLTKIVNIIEKKLMEDDYTSLDLAAALLKMSMGDDNEDIIDNYMPARSLDDLDS
                     YGRNSRGRDRGRNNSRRKGATDRAAVDYVLNGGEERMARLFINIGKSQRVTPGDILGA
                     VAGESGIPGRLVGSIDMYDGYTFVDVPAEYAEDVLNAMSHARIKGKHIHVEKANSKR"
     gene            complement(2412..3371)
                     /locus_tag="ACZPCL_11910"
     CDS             complement(2412..3371)
                     /locus_tag="ACZPCL_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009536038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family putative permease subunit"
                     /protein_id="HIR1163207.1"
                     /translation="MGNNEIDEDVMPVFLINGFLEAGKTQFLEFTMDQEYFQTEGKTL
                     LIVCEEGDTEYDEKKLKKHQTAVVYVDELSKLTPAYLNELEVIYRPERVLLEWNGMWN
                     QDELKLPDDWNIYQQITIIDGSTFDLYIQNMKPLLGAMLRNSELVIVNRCDGIADEKL
                     TSYRRTIRAMSRESEIVLEDKNGEIEQATLEEDLPYDINADVIEIKPADYGIWYIDCM
                     DQPERYKGKTVEFTAMVLKSPKFPKGQFVPGRMAMTCCEADMTFLGFMCKWKDAEKYK
                     TKEWVKVRAKVGVEYQRDYHGEGPVLYAENVEKAAEIKDIVQF"
     gene            complement(3384..>4496)
                     /locus_tag="ACZPCL_11915"
     CDS             complement(3384..>4496)
                     /locus_tag="ACZPCL_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR1163208.1"
                     /translation="YVREEEEVVLIENEFGEIGIDGGFLKDSGIEIREMNSGCICCSL
                     VGDFGKSLSEVLTKYKPDRIIIEPSGVGKLSDVMKAVIDVSADMDVALNSAVTIVDAA
                     KCKMYMKNFGEFFNNQIENAGTVVLSRTDITDTAKIQKDVEMIREKNPKAAIVTTPLA
                     ELGGSQLLEIIEKRDTMLDDLLEEVRESHHHHDDDDDCCCGHDHDHEHHHHDHDEEEE
                     HEHHHHHDHDDDCCCGHDHEHHHDHDEEEEHEHHHHHDHDDDCCCGHDHDHEHHHHHH
                     ADEVFTSWGMETIVPVTKDQLEDILKRLAETKEFGDVLRAKGMLPTENPGEWLYFDLV
                     PEQYEIRDGKPDYTGKVCVIGASLKEEELNKVFGRG"
BASE COUNT         1037 a         1126 c          918 g         1415 t
ORIGIN      
        1 ctttcctgtt cagaatagag ataccaaaga tgctttcatt gctgccggaa aggtcatttt
       61 catctacttc atatgttcct gtttcataat ggatccctgc tgcgtctaac agcctcatta
      121 cgttggtcct tacttctgct tctgcttttt ttcctttcaa atttatcact ccctgcatat
      181 tgtaaagaca ccctggtagg tttcagagtg tcttttactt ttctgatttg aatttttatc
      241 gtttggaatt tgctttttct acgtggatat gtttgccttt gatgcgggca tggctcatgg
      301 catttaatac atcttctgca tattcagccg gtacatctac gaaagtatag ccgtcataca
      361 tatcaatact tcctaccaga cgtcctggaa taccggactc tcctgcaact gcgcccagga
      421 tatctcccgg tgttacacgc tgggacttac cgatattgat gaacagtctt gccatgcgct
      481 cttcaccgcc gtttaataca tagtcaacag ctgctcggtc ggtagcgcct ttgcggcggc
      541 tgttatttct gccacggtct ctgcctctgg aatttctgcc atagctgtcc agatcgtcta
      601 aggatcttgc aggcatatag ttgtcaatga tatcttcatt gtcatcgccc atgctcattt
      661 ttaagagggc tgcagccaga tctaaggaag tgtaatcatc ttccatcagc ttcttttcaa
      721 tgatatttac gatctttgtg aggtctgcat catttaaaat gtcctgaacc tggtctaaga
      781 tcttgtctgc cttgatctcg gtaatgtcat ttaaggacgg gattgcctgt gggatgatct
      841 tggtcttgca gtagcgctgg atgtcacgaa gcttgtaaac ttccttgcca actaccaggc
      901 tgaatgcctt tccttctctt cctgctcttc cggtacgacc gatacggtgt acataatatt
      961 catcatcctg tgggatatcg tagttaaata cagcttctac atttcctacg tcaatgcctc
     1021 ttgctgctac gtctgtagca atcaggatat cggtacgtcc gtttctaaag ctttccataa
     1081 cgcggtcacg ctgtacctgc tttaaatcac cgtgaaggcc ttctgcaaaa taacctcttc
     1141 cctgaagtgc ctgaaccagt tcatctacct ggcgcttggt gttgcagaat acgatagaca
     1201 gctttgggga gtacatgtct aacagacggc acataacctc tactttgttt tttggcttta
     1261 cttcataata atactgggtc actttcggta cagtcagttc ttttttgatc acgcggactg
     1321 ttaccggatc tttctggaac ttctttgcaa tgtcagcaat ggcctgaggc atggttgcgg
     1381 agaacatcag ggtctggcgc tcttccggca gctggcttaa aatggtctcc atatcctcta
     1441 aaaagcccat gttcaacatt tcgtctgctt cgtctaaaac tacggtatgt acatggtcca
     1501 tttttacggt cttacggcgc atatgatcca tcacacggcc cggggttcct actacgatct
     1561 gggtgccatc ttttaaggcg cggatctgac ggacaatgtc ctgaccgccg tatacaggca
     1621 gaaccttgat gccgtgcata aattttgcaa aacggcgcag ttcttcagct acctggattg
     1681 ccagctctct ggttggaagc aggacaatgg cctgaagctt ctttaccttc gggtctacct
     1741 tctgtaacag aggaagtccg aaagcagcgg tctttcctgt tcctgtctgt gcctgaccga
     1801 tcatgtctct gccttctaat acaacaggga tcgcctgagc ctggatcgga gaagcttctt
     1861 caaagcccat ctcggtaata gctcttatga tacggtcatc taactgtaat tcttcaaatt
     1921 ttgttacatc cattaattgt aaatatcctc tttctattct taatctaagt tccgcaggaa
     1981 tatgggcctt aatttttcaa catgtcattg tccactggcc ttcgatttgc acatgtccgc
     2041 ctgtgtcgga ttgcatccgc cacattcgta catctgcact caggatgtgg acaatcgaca
     2101 ggttgaaaac ggcccatatt cctgcattga gcacgtaagt gttcgtcttc cagaacgctt
     2161 acgtgcgaaa gcacttcgtt gcacttagtg aggtattcca cgaacttagt gcaattgaag
     2221 ttaccatata tttttaacat gcaatagcaa gaaaatgaga ggcgaaaacg gcacgcacat
     2281 gcaggccttt catcctctca ctttcgtaca atcaataact atatcagagt tttgttttca
     2341 tgtcaatgta agaacttaca caactgttct tccttacact tcctgaaaat agcattttca
     2401 caagaacatc actagaactg cacaatgtcc ttgatctcag cggctttttc cacattttcg
     2461 gcatagagga caggtccttc gccgtggtaa tctctctggt attctacgcc gactttagcg
     2521 cgtaccttta cccattcttt ggtcttgtac ttttctgcgt ctttccattt gcacatgaaa
     2581 cctaagaatg tcatatctgc ctcacagcag gtcatagcca tacgtcccgg aacgaactgt
     2641 ccctttggga atttcgggct ttttaatacc attgcggtaa attcaacggt ctttcctttg
     2701 tagcgttccg gctgatccat acagtcgatg taccagatac cgtagtctgc aggcttgatc
     2761 tcaattacat ctgcattgat atcatatgga agatcttctt ccagggttgc ctgctcgatc
     2821 tcgccgttct tatcttccag aacgatctcg ctttcacggc tcatagcgcg gatggtacgg
     2881 cgataggaag tcagcttctc atctgcaatg ccatcgcagc ggtttacaat gaccagttcg
     2941 gaattacgaa gcatagcgcc aaggagcggc ttcatattct ggatgtacag atcaaaggta
     3001 gaaccatcga taatggtgat ctgctggtaa atgttccagt catccggcag tttcagctcg
     3061 tcctggttcc acataccgtt ccactctaag agcacacgct ccgggcggta gatcacttcc
     3121 agctcattta agtaagccgg tgtcagtttg gaaagttcat ctacatatac aacagcagtc
     3181 tgatgcttct ttaacttctt ttcatcatat tctgtatccc cttcttcgca gacgatcaga
     3241 agtgtttttc cctctgtctg gaagtattcc tggtccatgg taaattccag aaactgggtt
     3301 tttccagctt ccagaaaacc gttgatcaga aatacgggca ttacatcttc atctatctcg
     3361 ttatttccca ttcttaaacc tcattagcct cttccgaata ctttgtttaa ttcttcttcc
     3421 ttcaggctgg cgccgatgac acaaacctta cctgtgtaat ccggctttcc gtcacggatc
     3481 tcatactgct ccggtaccag gtcaaagtac agccactcgc caggattttc tgtaggaagc
     3541 attccctttg ctcttaaaac atcgccaaat tccttggtct ctgccagacg ctttaagata
     3601 tcttccagct gatcctttgt tacaggaacg atggtctcca tgccccagct ggtgaatact
     3661 tcgtctgcat gatggtgatg atgatgttcg tgatcatggt catggccgca gcagcagtca
     3721 tcgtcgtggt catgatgatg gtgatgttca tgttcttctt cttcatcatg atcgtgatga
     3781 tgctcatgat catggccaca gcagcaatcg tcatcgtggt catgatgatg gtgatgctca
     3841 tgctcttctt cctcgtcatg gtcatggtga tgatgctcat gatcgtggtc atggccgcag
     3901 cagcaatcgt cgtcatcatc atggtgatga tggctttctc ttacttcctc taacaggtca
     3961 tctaacatgg tatctctctt ctcgatgatc tcaagaagct ggcttccgcc cagttctgct
     4021 aatggagtgg taacgatagc agcctttgga tttttctcac ggatcatctc aacatccttc
     4081 tggatctttg cagtatcagt aatatctgta cggctgagta caacagttcc tgcattctcg
     4141 atctggttgt tgaagaattc accgaagttc ttcatataca ttttgcactt tgcagcatct
     4201 acgatggtaa ctgcgctgtt taatgcaaca tccatatctg cagatacatc aataacagcc
     4261 ttcataacgt cggaaagctt gccaacgcct gatggttcga tgatgatacg gtctggtttg
     4321 tacttggtca gtacctcaga taaggacttt ccgaaatcac ctactaagga gcagcagata
     4381 cagccggagt tcatctcacg gatctcgata ccggagtctt ttaaaaatcc gccgtcaata
     4441 ccgatctcgc caaattcatt ttcaatgagg actacctcct cctcctcccg tacata
//
LOCUS       DBMDUA010000208         4387 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7320_length_4387_cov_1.778115, whole genome shotgun sequence.
ACCESSION   DBMDUA010000208 DBMDUA010000000
VERSION     DBMDUA010000208.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7320_length_4387_cov_1.778115"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="ACZPCL_11920"
     CDS             complement(<1..919)
                     /locus_tag="ACZPCL_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HIR1163209.1"
                     /translation="MVKNEKEGILLESGTNEIEVMKFTVMGEFYGINVAKVQEIIMSE
                     KVKPMPHANPAVEGIFKPRDELITVINLAKYLTGNDRENGPRDLFIVTNFNKMTVAFR
                     VDSIEGISRISWKDIQKPDKTIANGADSVATGIAQCGKELVIILDFEKIVAEIAPETT
                     IQVTDVDKMGDRPICNSPIVIAEDSILLSKMIDDSLERAGFTNIKNFSNGQEAWDYLS
                     QIHNDSDLYDKVNLVITDIEMPEMDGHRLTKLIKDDEHLKKIPVIIFSSLINDQMRQK
                     GKELGADEQLSKPEIGHRRTGSAQLLRQPY"
     gene            complement(991..1479)
                     /locus_tag="ACZPCL_11925"
     CDS             complement(991..1479)
                     /locus_tag="ACZPCL_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HIR1163210.1"
                     /translation="MIGGTELKVGIGDMKFSRGEGRIITYALGSCIGITFYDPVTRLG
                     ALLHIMLPAQFERRDSNPFKFADSGIRETLRKLKIFGMVKSRMVVKIAGGAKMFEISG
                     NSSFGNIGQRNAESVKKVMAQEGICISAEDTGGNYARTMLLDLATGDVIVRTVGRPER
                     HL"
     gene            complement(1479..2099)
                     /locus_tag="ACZPCL_11930"
     CDS             complement(1479..2099)
                     /locus_tag="ACZPCL_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HIR1163211.1"
                     /translation="MEIKRYEDMDLQALDVMREIGSIGTSHAATALSKLLQKEVRISI
                     PSVNILGYNDAMNRIGDAEELVAAALVKMSGDIEGLMLFLFDLDFANEVLEKFLGSRY
                     KSFLDMDEMARSALVEVGNIIICSYINAFSLLVNVDVELSVPSATINMLGGILMVPIA
                     EYGYETDKLMYCNADFVIGGKTLSDWLLMLPDIRSLNDILERLGVS"
     gene            complement(2108..2899)
                     /locus_tag="ACZPCL_11935"
     CDS             complement(2108..2899)
                     /locus_tag="ACZPCL_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HIR1163212.1"
                     /translation="MNMSFYTGAAGAGQFTKKLSVVSNNLANINNTGFKPKTAAFSEL
                     IHYNLNDSENAQTELQTGAGARVERTWTDFSTAGATQTGKEYDYAIMEPNAFFMIQDP
                     TSGEITYTRNGHFHRGEREDGFYLTTEDGKLVLDQNQQPLKLEVTDVEKLQEESESED
                     NDYDTDDTDDTENDTDLPKVSVYTFSNPSRLLSVGDNEYKTQDGMEASLKSDAALQKG
                     ALENSGTDMAKEMVHVIECQRAFSYAVKMITTSDDIEGTINSLRG"
     gene            complement(2913..3710)
                     /locus_tag="ACZPCL_11940"
     CDS             complement(2913..3710)
                     /locus_tag="ACZPCL_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HIR1163213.1"
                     /translation="MYQGFYNLASGMLTQSRNLNTISNNMVNIQTPGYKNETMVSSTF
                     KEEMLYRTGQYNKENPTQLASVSKIKTAKQVYTNYDQGGLEQTDGIYDFALNGNGFFC
                     IQTSQGIRYTRNGSFSVDKDGYLVLNQAGRVLNKEGQPVQIKNENFTVDSRGHITAAI
                     TNQSDGEESGSQEVTIEDYGTLKVVDFPDQRQLHAEDNGLFSTAQAETTPADTTVQWK
                     MLEKSNVDMVEQMTAMMSSQRALQSAAQSLKMYDQIMSKSVSDVGKL"
     gene            complement(3691..>4387)
                     /locus_tag="ACZPCL_11945"
     CDS             complement(3691..>4387)
                     /locus_tag="ACZPCL_11945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1163214.1"
                     /translation="TMATKLYDLYSTTMQMEDAVNEGVVAIIKGIDMYDSDKNIKFST
                     YITTRVRGAMLDYVRKQEWMPRGFYKQLGIIESACEDMKLELGRTPTVDELAKHLGIE
                     KKRCQRIISMKNRGNIQSLDLLLENHNTTKGVQIINNVNNETQTQPEDACLWEEDMRV
                     LAEGIAQLNEKEQKTLALHYMEGLKMEQVGRVIGVSQPRASQIHTIALRKLRQFIEKK
                     NGKGIKSDVSGIL"
BASE COUNT         1041 a         1096 c          793 g         1457 t
ORIGIN      
        1 cgtatggttg cctcaggagt tgagcgcttc ccgtcctacg atgcccgatc tccggctttg
       61 aaagctgctc atctgcccct aattcttttc ccttctgcct catctgatca ttgatcaggg
      121 aagagaagat gataaccggg attttcttta aatgttcgtc atctttgatc agctttgtta
      181 gcctgtgacc atccatttcc ggcatttcga tatctgtaat gaccagattg accttatcat
      241 acagatcaga atcattatgt atttgtgaaa gataatccca tgcctcctgg ccattggaaa
      301 aattctttat attggtaaat ccggcccttt ccagggaatc atcgatcatt ttacttaaaa
      361 gaatggaatc ttccgcgatc acaataggac tgttgcagat agggcggtct cccatcttat
      421 ctacatctgt cacttgtata gttgtttccg gtgcaatctc tgccacgatc ttttcaaaat
      481 ccaagatgat caccagttct tttccgcact gggcaatacc ggtagcaaca ctgtcagctc
      541 catttgcaat ggtcttatct ggcttctgta tatctttcca tgaaatcctg ctgatacctt
      601 cgatactgtc taccctgaaa gcgaccgtca ttttattaaa atttgttacg ataaacaaat
      661 ctcttggccc attttcccgg tcatttcctg taagatattt tgccaggttg atcactgtga
      721 tcagctcatc ccttggttta aagatgcctt ctactgccgg gtttgcatga ggcatcggtt
      781 ttaccttttc actcatgatg atctcctgga cttttgccac attaatgcca taaaattcac
      841 ccataactgt gaatttcatt acttcgatct cgtttgttcc tgattctaaa agaatccctt
      901 ctttttcatt tttgaccatg atcttctctc cttttttctg taaacaggcc tgtcacctga
      961 tggtgacacc accataaatg aaagccctgg ctatagatgg cgttccggtc gtcccactgt
     1021 acgcacgatc acatcacctg ttgcaagatc taaaagcatg gtacgggcat aatttcctcc
     1081 tgtatcctct gccgagatac agattccttc ctgggccatg acttttttca cactttccgc
     1141 atttctctgc ccaatgtttc caaagctgct gtttccgctg atctcaaaca tcttggctcc
     1201 ccctgctatc ttgactacca tccggctttt taccataccg aatattttca gctttctgag
     1261 agtttccctg atgccgctgt ctgcaaattt aaatggattg gaatcccttc tttcaaactg
     1321 ggccggaagc atgatgtgca gaagtgctcc caggcgggtg actggatcat aaaaggttat
     1381 cccgatgcag gagcccaaag cataggttat gatccttcct tcccctcttg aaaacttcat
     1441 atcaccgatt cccactttta attctgtgcc tccgatcatt atgaaactcc caacctctct
     1501 aaaatatcgt ttagagaacg aatatccggc agcatgagca gccagtcaga aagtgttttt
     1561 ccgccgatca caaaatctgc attgcaatac atcagtttat ctgtttcata tccatactca
     1621 gcaataggta ccattaagat cccacccagc atattgatgg tagcacttgg aacagacagt
     1681 tccacatcta catttaccag cagtgaaaaa gcattgatat aagaacagat gatgatgttt
     1741 cccacttcta ccagcgcgga tctggccatt tcgtccatat ccagaaagga cttataacgg
     1801 cttccaagga atttttccag cacttcgtta gcaaaatcca gatcaaaaag gaacagcatc
     1861 aacccttcaa tgtcccctga catttttaca agagctgcag caacgagctc ctccgcatca
     1921 ccgatccggt tcatggcatc attgtatccc aggatattca cagatgggat cgagatccgg
     1981 acttctttct gaaggagctt tgaaagagca gttgctgcat gactggtacc gatactgccg
     2041 atctcccgca ttacatccag tgcctgcagg tccatatctt catatcgctt tatttccatt
     2101 ttttacctta tcctctcagt gaattgatcg ttccttctat atcatctgat gtagtgatca
     2161 tttttacagc ataagaaaag gctctctgac attcgatcac atgaaccatt tcttttgcca
     2221 tatctgtgcc tgaattctcc agtgcacctt tttgcagggc tgcatctgat tttagggacg
     2281 cttccattcc atcctgtgtt ttgtattcat tatcccctac gcttaacaga cggcttggat
     2341 ttgaaaatgt gtatacactt accttaggaa ggtcagtatc attctctgtg tcatctgtat
     2401 catcagtatc gtagtcattg tcttctgact ctgattcctc ctgcagcttc tctacatctg
     2461 ttacctccag tttaagaggc tgctggttct ggtccagcac cagcttgcca tcttctgttg
     2521 taagataaaa tccatcttca cgttctcccc tgtgaaaatg accgtttctt gtataggtga
     2581 tttcacctga tgtaggatcc tgtatcataa aaaatgcatt aggctccata attgcataat
     2641 catattcttt tccggtctga gtagcaccgg ctgtactaaa atccgtccat gtgcgttcta
     2701 ccctggctcc tgcacctgtc tgcagctctg tctgggcatt ttcagaatca ttgagattat
     2761 agtggatcag ttcagaaaaa gccgcggttt taggtttaaa gcctgtattg ttgatattgg
     2821 ccagattatt ggatacaact gacaattttt ttgtaaactg tccggctcct gctgctcctg
     2881 tataaaatga catattcatt tccttatcct ccttacagct taccgacatc tgacacagac
     2941 ttactcatga tctgatcata cattttcagt gactgggctg cgctttgaag cgctctctgg
     3001 gatgacatca tagccgtcat ctgctccacc atgtctacat tggatttttc cagcattttc
     3061 cactgaacag ttgtatctgc aggtgttgtt tctgcctgcg cagtagagaa aagtccatta
     3121 tcctctgcat gaagctgcct ctgatctgga aaatccacta ctttcaaagt tccataatcc
     3181 tctatggtaa cttcctggct gccgctttct tcaccatcag actgatttgt aatagcagca
     3241 gtaatatgtc ctctggaatc tactgtaaag ttctcatttt tgatctggac aggctgtcct
     3301 tctttgttta agactctgcc tgcctggttt aaaaccagat atccatcttt atctactgaa
     3361 aatgaaccat ttcttgtata ccggatgccc tgtgatgtct ggatgcaaaa aaagccattt
     3421 ccatttaagg caaaatcata aattccatct gtctgttcca gaccgccctg gtcataattg
     3481 gtataaacct gctttgctgt ctttattttt gaaacagagg ctagctgggt tggattttct
     3541 ttgttatatt gtcctgtacg gtaaagcatt tcctctttaa aagtactgct taccattgtc
     3601 tcgttcttat atccaggagt ctggatattc accatattat tgctgatggt atttaagttc
     3661 ctgctctggg tcagcatgcc tgacgccaga ttataaaatc cctgatacat ctgactttat
     3721 cccctttccg tttttctttt ctataaactg gcgcagctta cgcaaggcga tcgtgtggat
     3781 ctgggatgcc ctgggctggc tgacacctat gacccggcct acctgttcca ttttcagtcc
     3841 ctccatataa tgtagtgcca atgttttctg ctctttctca tttaactggg ctatgccttc
     3901 tgcaagcacc cgcatatcct cttcccacaa gcatgcatcc tcaggctgtg tctgggtttc
     3961 attgtttaca ttgtttatga tctggactcc cttagttgta ttatggtttt ccagcagaag
     4021 atcaagagac tggatatttc ccctgttttt catagaaatg attctctggc agcgtttttt
     4081 ttctattcca agatgctttg ccagctcatc aactgttggt gttcttccta attccagctt
     4141 catgtcctcg catgcagatt ctataatacc caactgcttg taaaatcccc ggggcatcca
     4201 ctcctgtttc cttacataat ccagcattgc tcctcttacc cgcgttgtga tgtatgtgga
     4261 aaacttaata ttcttatccg aatcatacat atcgatccct tttatgattg ccaccacacc
     4321 ttcgttcact gcatcttcca tctgcatggt ggtagaataa agatcatata gtttcgttgc
     4381 catggtt
//
LOCUS       DBMDUA010000209         4373 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7351_length_4373_cov_2.404026, whole genome shotgun sequence.
ACCESSION   DBMDUA010000209 DBMDUA010000000
VERSION     DBMDUA010000209.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7351_length_4373_cov_2.404026"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            225..506
                     /locus_tag="ACZPCL_11950"
     CDS             225..506
                     /locus_tag="ACZPCL_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR1163215.1"
                     /translation="MLKRRRRLMSDQKAQIIGELQRKGKRITGQRKILLDVILEGKWS
                     SCKEIYYMASKKDPTIGLATVYRMVGVLEEMGFLSRGYRYSYPVEDKIS"
     gene            complement(681..1070)
                     /locus_tag="ACZPCL_11955"
     CDS             complement(681..1070)
                     /locus_tag="ACZPCL_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR1163216.1"
                     /translation="MTPGAYCDGIQHVGIPTDHLENTIHFYEDLGFQVILRACVPEKN
                     QHVAFLQLGNLVIETYEDPVALCDGAINHIALNCLDIEKAYEAALNQKHLVLSNGIEA
                     LDFWERGVYFFIIQGPNKERIEFCQKL"
     gene            complement(1109..1849)
                     /locus_tag="ACZPCL_11960"
     CDS             complement(1109..1849)
                     /locus_tag="ACZPCL_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012958.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HIR1163217.1"
                     /translation="MALILDHFYSAALTECNSLTAIVPVENNEKSSYPVLWLLPPAGC
                     DHTAWQRHTAVEDLADELGIIIVMPDLKLSYGLDMIHGFAYFQMLTKELPEMVADYFP
                     ADLGFQMIAGVKEGGYTALNAALSVPGQYHMAASYSCGSLTDETFDPELDKQLDNAFG
                     TTDLRTLNGTDKDLKMLVQTSKDHNMVLSLEYSEKDDYKASSALLADCILKSSFSNRS
                     VRVQKEALTWSKWAEALKTDVKNALKQL"
     gene            complement(1851..2669)
                     /locus_tag="ACZPCL_11965"
     CDS             complement(1851..2669)
                     /locus_tag="ACZPCL_11965"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1163218.1"
                     /translation="MALLHVNFHSKVLNMDTKIGVIMPQKMQGIGLAGSEQADSFPVL
                     WLLHGRSDDYTIWMRRTSIERYAAPLGLMVIMPEVSYSRYINMKHGPRYYDYISKDLP
                     EFCAKLFPKMSRKREDNYIAGLSMGGGGAMWVGLNNPDKYGRICMLSTGGVAPLEHLW
                     RNKSGSDYMFKKCNEDIYGTSDSDSLAGTEYDILKLIQDTAKEHTILPKIYHAMGTED
                     VRYPVAMEIKKTFENLPGNPYEYEYHEGPGVHEWVFWDEWIQHFLDTLALKGGN"
     gene            complement(2682..3515)
                     /locus_tag="ACZPCL_11970"
     CDS             complement(2682..3515)
                     /locus_tag="ACZPCL_11970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1163219.1"
                     /translation="MKKRKILHALIYAVLTLGALLVFFPFYLTVVSSLKDMSDIFSNF
                     FGLPRHIVFDNFAYVLQRPDYFIALKNSAIITGVAIVAMVFFLPMCAYAISRRRFTSR
                     FYSFLYFFMVAGIFIPFQVKMLPMVKLMSNIGLLNRTGAILFYIANSTCEGIFLLVGY
                     LASMPIDLEEAAYIDGATTNQVFYKIVRPIMKPIISTVVIKNSLWIWNDYFMPSLVLN
                     QSNLNRTLTLYQYSFRTENSTNFTIVFAVMLLSILPIFIVYCLFQKQIVSGMMEGAVK
                     G"
     gene            complement(3531..4175)
                     /locus_tag="ACZPCL_11975"
     CDS             complement(3531..4175)
                     /locus_tag="ACZPCL_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1163220.1"
                     /translation="MLIICVMLLSVVLALLMNTKIRGQNCFRAIYFFPACVSMLTIGL
                     IFNYIFFQGIPSLGTSLGIEALQKNILSGRSTAIYGILITNIWKSVAIPTVLVISALQ
                     TIPGEIIEAAIVDGANGRQRFYYITLRYILPILSIIFVLVLKEGLMIYDYIMAMTSGG
                     PAGATESITLSIYRIGFEDMKFGYAISQAMIVAVIIAVISITQIRFTDKKKIYD"
BASE COUNT         1365 a          891 c          949 g         1168 t
ORIGIN      
        1 ctcaagcaac aggtattgtc tggagaacct taacagatag tacttgttgc ttatttttta
       61 tcaaaaaaca gttgacaaga aagttagttg gtgctaatct tataaacggt tagataaatt
      121 agtctaaact aactataaca cagatccaaa atgtataaaa taaaaaagat gaaacagact
      181 atgtaggaaa tatggataaa aggcatcaaa acaggatatc cgtgatgctt aaaagaagga
      241 ggagacttat gtcagatcag aaagcgcaga tcataggaga acttcagaga aaaggaaaaa
      301 ggatcacagg acagagaaaa atccttctgg atgttatatt agaaggaaaa tggtccagct
      361 gtaaggaaat ctactacatg gcctccaaaa aggatccaac tatcggcttg gcgactgttt
      421 acagaatggt aggcgtatta gaggaaatgg gatttttaag cagaggatac agatattctt
      481 atccagttga agataagatc agctaaagcc gggatagttt acttacagaa actgcctcat
      541 ggcaggttct tctgtatgca tagcaaaaac aggctgcatg tcatataaaa atgttcagcc
      601 tgtttttgct ttatccacac agaaaacgcg ccagaaggcg cgttctgtga tctatacaaa
      661 tactccccta aaaatcaaat ttacagtttc tggcaaaact cgatccgctc tttgtttggt
      721 ccctggatga taaagaagta cacacctctt tcccagaaat caagagcttc aatgccattg
      781 ctcaacacca gatgcttctg gtttaaagca gcttcgtagg ctttttcaat atccaggcag
      841 tttaaggcaa tgtggttaat ggctccgtca cagagagcca caggatcctc ataagtttcg
      901 atcaccagat ttcctaactg caaaaaagct acatgctggt tcttttccgg gacacaagcc
      961 cttaagatga cctggaatcc cagatcctcg taaaaatgga tcgtattttc taaatgatct
     1021 gttgggattc caacatgctg gatcccgtca caataagcac caggagtcat atgatatcat
     1081 cctttcttgt gaagcttgat ctgcctgatc aaagctgttt caaagcattt ttaacatcgg
     1141 tttttaaagc ttcagcccat tttgaccagg taagagcttc tttttgtacc cgtacagacc
     1201 ggttgctaaa agaacttttc aggatgcagt cagctaaaag agcagaggaa gctttatagt
     1261 catctttctc agaatactct aaagaaagga ccatattgtg atctttggat gtttgaacca
     1321 gcattttcag atccttatcc gtgccattca atgtgcgaag atcagtggtt ccaaatgcat
     1381 tatccagctg cttatccagt tctgggtcaa aagtctcgtc tgtaagagaa ccgcagctgt
     1441 aagatgctgc catatggtac tgtccaggaa cagaaagagc tgcgtttaag gctgtatagc
     1501 ctccttcctt tacaccggcg atcatctgga agcccaggtc agccgggaaa tagtcagcaa
     1561 ccatttccgg aagctctttt gtaagcatct ggaaataagc aaatccatgg atcatatcaa
     1621 gcccgtagct taatttcaga tccggcatca caatgataat gcccagttca tctgccagat
     1681 cctctacagc tgtatggcgc tgccatgctg tgtggtcgca gcctgcaggg ggaagcagcc
     1741 ataaaacagg ataagagctt ttttcattgt tttccacagg aacaatagct gtcagggaat
     1801 tgcattccgt aagggcagct gaataaaagt ggtccagtat gagagccatg ttagtttcct
     1861 cctttcaacg caagagtgtc aaggaaatgt tggatccatt catcccagaa gacccactca
     1921 tggacaccgg gaccttcgtg gtattcatac tcatatggat taccgggaag gttttcaaag
     1981 gtttttttga tctccatggc aacaggatat cggacatctt ctgtacccat agcgtggtat
     2041 atttttggca gtatagtatg ctctttcgca gtatcctgga tcagctttaa aatgtcatat
     2101 tctgttcctg caagggaatc agaatcagag gtgccgtaaa tgtcctcatt gcatttttta
     2161 aacatatagt cactgccact tttatttctc caaaggtgct ctagcggagc aactccgcca
     2221 gtagaaagca tgcagatccg gccatatttg tccggattgt tcaggcctac ccacatagcg
     2281 ccgccgccgc ccatggacag tccggcaatg tagttatctt cccgtttccg gctcattttc
     2341 gggaataatt ttgcacaaaa ttccggcaaa tcctttgaaa tatagtcata atagcgggga
     2401 ccatgcttca tattgatata acggctgtag ctaacctccg gcataatgac catcagacct
     2461 aaaggagctg cgtagcgttc gatagaagta cggcgcatcc agatggtata atcatcactg
     2521 cgtccgtgca acagccacag aaccgggaag ctgtctgcct gctcgcttcc ggcaagaccg
     2581 atgccctgca ttttctgggg catgatcacg ccgatcttag tgtccatatt caggaccttt
     2641 gagtggaaat tcacatgtaa taaagccaat gtatatacct cctaaccttt gacagcacct
     2701 tccatcatgc cggagacgat ctgtttctgg aacaggcagt aaacgataaa gatcggcagg
     2761 atagaaagaa gcataacagc aaatacaata gtgaaattgg tagagttttc tgtacggaat
     2821 gaatactggt acagagtcag tgtacggttt aagttagact ggtttaatac aagggatggc
     2881 ataaaatagt cattccagat ccacagggag tttttgataa caaccgtgga gatgataggt
     2941 ttcataatag ggcgcacaat cttataaaac acctggtttg tagtagcacc gtcaatatat
     3001 gcagcttctt ccagatcaat aggcatggaa gccagatagc ctactaagag gaagatacct
     3061 tcgcaggtag agtttgcaat gtagaaaaga atggcaccag tcctgttaag aagaccgata
     3121 ttgctcatta actttaccat aggaagcatt tttacctgga aagggataaa aataccggct
     3181 accatgaaaa agtaaagaaa gctgtaaaaa cgggatgtaa atcttctgcg ggaaatggca
     3241 taagcacaca tgggaaggaa aaataccatg gcaacaatgg ctacaccagt gatgatcgca
     3301 gagtttttca gtgcgatgaa ataatccggt ctctggagta cataagcaaa attgtcaaaa
     3361 acaatatgcc ttggaagacc gaagaaattg ctgaagatgt cgctcatatc ctttaaagaa
     3421 gaaactacgg taagataaaa tgggaaaaat acaaggagag cgcccagagt cagcactgca
     3481 tagatcagag catgtaagat ttttcttttt ttcatgggat acctcctgtt ttaatcgtag
     3541 attttctttt tgtcagtaaa tcgaatctgg gtaatggaaa taactgcaat gataacggca
     3601 acgatcatag cctgggaaat ggcatatcca aacttcatgt cctcaaatcc gatacggtaa
     3661 atggaaagag tgatactttc tgttgcgcct gccggtccgc cggaggtcat tgccataatg
     3721 taatcataga tcataagtcc ttctttcagg acaaggacaa aaataatact taaaataggc
     3781 agaatgtagc gaagcgtgat gtagtaaaag cgctgacggc catttgcacc atctacaatg
     3841 gctgcttcga tgatctcacc tggaatggtc tgtaaagcag aaatgactaa aactgttgga
     3901 attgcaacac ttttccatat atttgtgatc aggatgccgt aaatagcagt gcttctgccg
     3961 gataaaatgt ttttctgtaa tgcttcaatc ccaagggaag ttccgagact ggggattccc
     4021 tgaaagaaaa tataattgaa gatcagtcca atggtcagca tactgacaca ggccgggaaa
     4081 aaataaatag ctctgaagca gttctggcca cggatctttg tgttcattaa cagcgcaagt
     4141 acaacagaca gcagcattac gcagatgatc agcattgcgg catatcgtat attaaatagt
     4201 acggctttcc ggaaacgatt gtcggataaa attttaatat agtttttcag gcctacaaat
     4261 ttatagttct taccgatgcc gttccagttg gtaaagctgt aatacagccc aaggacaatt
     4321 ggaacaatag agagaaaaga atgaagaaag tattatcatt agtagctttg gcc
//
LOCUS       DBMDUA010000210         4356 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7383_length_4356_cov_2.363807, whole genome shotgun sequence.
ACCESSION   DBMDUA010000210 DBMDUA010000000
VERSION     DBMDUA010000210.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7383_length_4356_cov_2.363807"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="ACZPCL_11980"
     CDS             complement(<1..493)
                     /locus_tag="ACZPCL_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1163221.1"
                     /translation="MKLKEQKIAQVVIGSGAAGFQSALRLYQNGEWDLTVVTENINAG
                     TSRNTGSDKQTYYKLTLSGKDPDSVENMAEDLFAGQCVDGDQALCEAALSARCFFALT
                     ELGVPFPCTEQGEFMGYKTDHDRGRRATSAGPYTSRLMTEALEKAVKEKEIRILDKMQ
                     VIKL"
     gene            complement(509..1276)
                     /locus_tag="ACZPCL_11985"
     CDS             complement(509..1276)
                     /locus_tag="ACZPCL_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HIR1163222.1"
                     /translation="MKKTAIITGASRGIGYAAAKKLGMDGCQVVLFATGEKERYKAAI
                     DGLTQAGITWHYVRGSLDCREDRERLIRETVEKFGRIDILVNNAGVAPKERKDLLEMS
                     EESFDRVLGINTKGTMFLTQLAAKQMIKQEQIFKSKGHIVNVGSCSAEVSSTSRGEYC
                     VSKAAVSMLTTLFADRLAPEGILVNEVRPGVIATDMTSVVTEKYDTMIKNGVFPIARW
                     GKAEDVAEMIAFFCSDSVLYTTGNYVDVDGGFHIKRL"
     gene            complement(1316..2671)
                     /locus_tag="ACZPCL_11990"
     CDS             complement(1316..2671)
                     /locus_tag="ACZPCL_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012927.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR1163223.1"
                     /translation="MSQERFHYKTLEEVKERAEELKVYLPFSSSTDILKTPLKVGNVT
                     FHNRMGIAPMEGADSLEDGSPSDYTIRRYVNEAIGGSALIWFEAISIVPEGRSSKTQL
                     LLTEENVESYKRMNEKIKEAGRKANGFEPYLIMQANHSGRYSNPDNRPAPMIAYRHPQ
                     LEQYRAADDSCIVTDDYLKSLEESFGKAALLAKKAGFDAVDIKSCHGYLLAELLSAYD
                     RPGQYGGNYENRTRLLKNGIKAAKVWEDENFQVTCRLGIYDGYEYPWGFGVSEGSGLK
                     PDLKEPIRLVKELYGNCGIQMMNLTMGNPYATTHVTRPFDMGKYEPEEHPFTGIGRMI
                     AGIGEVKKAVPEMVIFGSAPTYLRQFADLYTAGAVEEGFCDGMLFGRMAFADPDYANE
                     IIKNGRIDPKRVCMTCGKCGDLIRAHKPTGCVIRDPKTFMPFYKEFLEIKKKQPANFR
                     G"
     gene            complement(2677..3492)
                     /locus_tag="ACZPCL_11995"
     CDS             complement(2677..3492)
                     /locus_tag="ACZPCL_11995"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1163224.1"
                     /translation="MEDFWMEHRYSSCCYQLEPHYEHTYQLVFLLKGKILYQVGEKQY
                     EVSKGGMILLNSLEEHTLKVLEYPYERYIIQINPLFFQKEINTPEIISIFIKRPENFS
                     HLLALTDPIWNYVYDVIQELEKEYREKRLYWEMYIGADLRRMFITIFRECEELLASVR
                     VDASAAIAYKVLNYLDHHYPEDISVTKLAETFFLNKHYIAHVFKAETGYSPMDYVISL
                     RMNRAKALLAETARSISEIAVECGYTDFNHFSKLFKKYAGMSPSRFRKTSGKE"
     gene            complement(3559..>4356)
                     /locus_tag="ACZPCL_12000"
     CDS             complement(3559..>4356)
                     /locus_tag="ACZPCL_12000"
                     /inference="COORDINATES: protein motif:HMM:NF018530.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-alanine antiporter-like transporter"
                     /protein_id="HIR1163225.1"
                     /translation="IDPSGSLTVEDTPSLNEEQVAAYKEAAASSISLGYTVAFPIGVL
                     VIVVMETLLPKVFRIDMEKEREKYQKELEENMAGEKKMKPQPLNFMILSFVAIIGIAV
                     GSITIPLGGFGSFSLGAAGGVLLAALILSYVGQIGPVNFRMEDKSLGIMYQMGLTFFM
                     AVVGLRYGYDVVMSLMGSGLSLAIAAIFVEAAAVLVSFLIGHYIFKLNWIILSGAIAG
                     GCTSAPGLGAAISSTGSEEPTAGYGAAQPFAILANVLLATLFFHFFL"
BASE COUNT         1139 a         1109 c          800 g         1308 t
ORIGIN      
        1 gaagtttgat cacctgcatc ttgtctaaga tccggatctc tttttctttt actgcctttt
       61 ccagagcttc tgtcatcagc cttgaagtat aaggacctgc gctggtagct ctccttcccc
      121 tgtcatggtc tgtcttatat cccataaact cgccctgttc tgtacatgga aaaggaaccc
      181 ccagttctgt aagggcaaaa aaacaacgtg ctgaaagagc tgcctcacac agcgcctgat
      241 caccgtccac acactgtcct gcaaacagat cctctgccat attttccaca ctgtccgggt
      301 cttttcctga caacgtcagt ttgtaataag tctgtttatc agaaccggta tttctggatg
      361 tacctgcatt tatattttcc gttacaaccg tcaggtccca ctccccgttc tggtacagcc
      421 ttaaggcaga ctgaaaacct gccgccccgc ttccgatgac cacttgtgcg attttttgct
      481 ctttcagttt cattgtgtaa gccctccttt atagcctctt gatgtgaaaa cctccgtcta
      541 catccacgta atttcctgtt gtataaagta cactgtcgga acagaaaaaa gcgatcatct
      601 ctgccacatc ttctgctttt ccccaacgcg caataggaaa tacaccattt ttgatcattg
      661 tatcgtattt ttctgtaaca acactggtca tatctgtggc aatgactccc ggacgcacct
      721 cattcaccag gatcccttcc ggtgccagac ggtccgcaaa cagtgtggta agcatggaaa
      781 ccgctgcttt tgaaacacag tattcccctc tggatgtaga cgaaacttct gcggaacagg
      841 aacccacatt tacaatatgt cccttgcttt taaagatctg ctcctgtttt atcatctgtt
      901 ttgcggccag ctgggtaaga aacatggttc cctttgtatt gatccccaaa acacggtcaa
      961 aactttcctc agacatctct aacaggtctt ttctctcttt tggcgcaact cccgcattat
     1021 ttaccagaat atctattctt ccgaattttt ctactgtttc cctgatcaga cgctccctgt
     1081 cttctctaca gtccaatgat cctcttacat aatgccaggt gatccctgcc tgtgttaatc
     1141 cgtctattgc tgctttataa cgttcttttt ctcctgtcgc aaaaaggact acctgacatc
     1201 cgtccattcc cagcttcttt gccgccgcat atccgatccc acggctggct cctgttataa
     1261 tcgccgtttt cttcatcttt tacctcttat ttctgtttgt tttacaaatt tatctttatc
     1321 ctctgaaatt agcaggctgt ttctttttta tttccagaaa ttctttataa aaaggcataa
     1381 acgtctttgg atcccggatc acacagcctg taggcttatg ggcacggatc agatccccac
     1441 attttccgca tgtcatacat actcttttag gatcaatacg gccgttttta atgatctcat
     1501 tggcataatc agggtcagca aaggccattc ttccaaaaag catcccatca caaaatcctt
     1561 cttctactgc ccccgctgta tacagatctg caaactggcg cagataagta ggtgcagaac
     1621 caaaaatgac catttcagga actgctttct ttacttcccc tattcctgcg atcatacggc
     1681 caatgcctgt aaaaggatgc tcctccggct catattttcc catatcaaaa ggtcttgtta
     1741 catgagtggt ggcatatggg tttcccatag tcaggttcat catctgaata ccacagtttc
     1801 cgtaaagttc tttcaccaga cggatcggtt ctttcagatc cggctttaac ccgcttccct
     1861 ctgaaacgcc aaagccccat ggatattcat atccgtcata aatccccagg cggcaggtca
     1921 cctggaagtt ttcatcttcc catacttttg ctgctttaat gccatttttt aacagtcttg
     1981 tacggttctc atagtttccg ccatactgcc ccggacggtc ataggcagat aaaagttctg
     2041 ccagcagata accatgacat gacttgatat ctactgcatc aaaaccggct tttttcgcca
     2101 gaagtgccgc ttttccaaaa ctttcttcca ggctctttaa ataatcatct gtaacaatac
     2161 aggaatcatc tgctgcccgg tactgctcta actgcggatg acgataggcg atcataggcg
     2221 ccggacggtt atccggatta gaataacggc cgctgtggtt tgcctgcatg atcagataag
     2281 gttcaaaacc attggctttg cgtcctgcct ctttgatttt ttcattcatc cgtttataac
     2341 tttccacatt ttcttctgta agaagaagct gtgttttgga agaacggcct tccggcacaa
     2401 tagaaatggc ttcaaaccag atcagggcac tgccgccaat agcttcattt acataacggc
     2461 ggatggtata atcagacggt gatccatctt ccaaagagtc agctccctcc attggcgcga
     2521 tccccatacg gttatgaaat gtaacatttc ccacttttaa gggagttttt aaaatatctg
     2581 tactggaaga aaatggcaaa tataccttca gttcttctgc ccgttccttt acttcttcca
     2641 gtgttttata gtgaaatctt tcctgtgaca taaaatctat tcctttcctg atgttttcct
     2701 gaaccggctt ggtgacattc cagcatattt tttaaacaat ttggaaaaat ggttaaaatc
     2761 cgtatagccg cactccactg ctatttccga tatggaacgg gcggtttccg ccaaaagggc
     2821 cttggcgcgg ttcatacgaa gggagatcac atagtccata ggactgtaac cggtctctgc
     2881 cttaaataca tgagcgatat aatgtttatt taagaaaaat gtttcagcta attttgtaac
     2941 agagatatct tccggataat gatgatccag atagttcagc actttataag caatggctgc
     3001 gctggcatcc acccgcacag aagccagaag ttcttcacat tccctgaaaa tggtaataaa
     3061 catgcgcctt aaatctgcac caatatacat ttcccagtaa agccgttttt cacggtattc
     3121 tttttccagt tcctggatca catcatacac ataattccag atgggatctg taagggcaag
     3181 aagatgagag aaattctcag gtcttttaat aaaaatggaa ataatctccg gcgtattgat
     3241 ctctttctga aaaaacagag gattgatctg gatgatatac cgttcatagg gatattccag
     3301 cacctttaat gtatgctcct ccagactgtt taaaaggatc atcccgcctt ttgacacttc
     3361 atactgtttt tcacctacct gataaaggat ttttcctttc agaagaaaca caagctgata
     3421 tgtatgctca taatgaggtt ccagctgata gcagcaggaa ctgtatctgt gttccatcca
     3481 gaaatcttcc ataactctcc cacctctctt taccccgcaa ttttctaagg gtatccatgc
     3541 cccattgtac cagatatcct acaaaaagaa atggaaaaac aaagttgcaa gcaatacatt
     3601 tgcaaggatc gcaaatggct gtgccgcgcc atatccggct gttggttcct cagaaccggt
     3661 agaactgatg gctgctccca gtccgggcgc ggaagtacag ccgcctgcaa tggcaccgct
     3721 taagatgatc cagttcagtt tgaaaatata atgaccgatc aggaagctta ccagcactgc
     3781 ggctgcttct acgaaaatag cggcaattgc cagtgaaagg ccggatccca ttaaagacat
     3841 aactacatca tatccatagc ggagtcctac tactgccata aagaaagtca gtcccatctg
     3901 atacatgata ccaaggcttt tatcttccat acggaaattt acaggtccga tctgtcccac
     3961 atagcttaag atcagtgctg ctaacaacac accgcctgct gcgcctaaag agaagcttcc
     4021 aaatccgcct aatggaatag taatacttcc aactgcaata ccgatgatcg caacaaaaga
     4081 gaggatcatg aagtttaatg gctgaggttt catcttcttt tcacctgcca tattttcttc
     4141 cagttctttc tgatatttct cccgctcttt ctccatatca atacgaaata cctttggaag
     4201 cagtgtttcc attactacga tcacaagaac accgataggg aacgctacag tataaccaag
     4261 gcttatagaa gaagcggctg cttctttgta agctgctacc tgttcttcat ttaaagatgg
     4321 ggtatcttcc acagtcagac ttcctgacgg atcgat
//
LOCUS       DBMDUA010000211         4346 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7404_length_4346_cov_2.229918, whole genome shotgun sequence.
ACCESSION   DBMDUA010000211 DBMDUA010000000
VERSION     DBMDUA010000211.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7404_length_4346_cov_2.229918"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="ACZPCL_12005"
     CDS             complement(<1..1217)
                     /locus_tag="ACZPCL_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR1163226.1"
                     /translation="MTAELISVGTEILMGNILNSNARYLAEKCASLGLDMYYQVTVGD
                     NYDRMMAVVKTALDRSDIVIVTGGLGPTEDDLTKEVCAEAMGMELEEDPHTRACLEAY
                     FKNNIYREIPENNWKMVTVPHGSIVLDNPNGMAPGLIMEKNGKTAILLPGPPNELYPL
                     FEGQVFPYLEKRQNSQLVSHMVKICGYGESQVEDKILDLIDKQTNPTIATYAKTAEVQ
                     VRLTAKAVTKEEGEALIAPVMAELFNRFGDCIFTTEEDVTLEMAVVDLLQKKHLTMAT
                     AESCTGGMIAARLVNVSGVSDVFMQGMVTYSNEAKVRLLGVKEETLKAHGAVSRETAL
                     EMALGGAERAQTDVCVAVTGLAGPGGGSKEKPVGLVYMACAVKGKAEVLEYHFKGNRG
                     KIREQSMMKALDLV"
     gene            complement(1221..1763)
                     /gene="pgsA"
                     /locus_tag="ACZPCL_12010"
     CDS             complement(1221..1763)
                     /gene="pgsA"
                     /locus_tag="ACZPCL_12010"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR1163227.1"
                     /translation="MNLPNKLTVMRVILIPFFVAALLYDNGSSQTMRVVANVIFIVAS
                     LTDLFDGKIARKYNLVTNFGKFMDPLADKLLVCSALICLIQLGQLPAWVVIIIISREF
                     IISGFRLVAADNGIVIAASYWGKFKTTFQMIAVILMIFNIPALKTVTFIMLAAAVVLT
                     IVSLVDYVAKNVEVLTKGGM"
     gene            complement(1747..3111)
                     /gene="rimO"
                     /locus_tag="ACZPCL_12015"
     CDS             complement(1747..3111)
                     /gene="rimO"
                     /locus_tag="ACZPCL_12015"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR1163228.1"
                     /translation="MEKIKLFCVSLGCDKNLVDTEKMLGVAGGLGVSFTDDETEADAI
                     LINTCCFIGDAKEESVNTILEMARYKEEGKCRALIVAGCLAQRYKQEIIDEIPEVDAI
                     LGTTSYEEIGNVLTRLFGEEKEKKEEHISCFHDLKELPETAKKRVMTTGGHYAFLKIA
                     EGCDKRCSYCIIPYLRGPYRSVPMEQLLAEARELAENGVRELILVAQETTLYGKDLYG
                     EKTLPKLLHELAQIPGIYWIRIQYCYPEEITDELIQAIKTEEKVCHYLDIPIQHASDR
                     ILKRMGRRTHKAELKERIGALRREIPDIALRTTFICGFPGETEEDHEELMEFVDEMEF
                     ERVGVFTYSAEEDTPAYSYPDQIPEEVKEERRADVMELQQEIAFEHCENMVGKVLDVM
                     IEGKVADEPAYVGRTYMDAPNVDGLIFVNADEMLMSGDFVRVKVTGANEYDLIGEVYH
                     ESAE"
     gene            complement(3198..3476)
                     /gene="rpoZ"
                     /locus_tag="ACZPCL_12020"
     CDS             complement(3198..3476)
                     /gene="rpoZ"
                     /locus_tag="ACZPCL_12020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009532104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR1163229.1"
                     /translation="MLHPSYTDLINVVNKDIEPGEQPVVQSRYSIVIAASKRARQLIA
                     GDDAMVAGAAGKKPLSIAIDELYQQKVKILPEEEPSAEEELDTTEEQA"
     gene            complement(3536..4180)
                     /gene="gmk"
                     /locus_tag="ACZPCL_12025"
     CDS             complement(3536..4180)
                     /gene="gmk"
                     /locus_tag="ACZPCL_12025"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR1163230.1"
                     /translation="MNHQGILVVVSGFSGAGKGTLMKELLKRYDNYALSISATTRAPR
                     EGETDGKEYFFVTKEQFEKMRDERKLVEYAQYVNNYYGTPKEYVEQKMAEGKDVILEI
                     EIQGALKVKKRFPDALLLFVTPPSAEELRRRLVGRGTETLEVINARLARAAEEASGME
                     AYDYLLINDDLDRCVEEMHQLIQLQHRKTSYHLDFLSKMREDLYHLDDKGFAKK"
     gene            complement(4197..>4346)
                     /locus_tag="ACZPCL_12030"
     CDS             complement(4197..>4346)
                     /locus_tag="ACZPCL_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HIR1163231.1"
                     /translation="GRKLDFIAQEMNREANTILSKSSDLAISDKAIALKTEIEKVREQ
                     IQNIE"
BASE COUNT         1045 a         1119 c          842 g         1340 t
ORIGIN      
        1 accaggtcca aagccttcat catagactgt tcccggatct ttcctctgtt tcccttaaaa
       61 tgatactcca gcacttctgc ctttcccttt acagcgcagg ccatgtatac aagacctacc
      121 ggtttttcct tgctaccgcc tccaggacct gcaagaccgg taacagccac acaaacatcg
      181 gtctgggccc gctctgctcc gcccagtgcc atttccaggg cagtttcacg gcttacagcg
      241 ccatgtgcct ttaaagtttc ctcttttaca cctaaaagac gtactttcgc ttcgttagaa
      301 taagtaacca ttccctgcat aaatacatcc gaaacgccgg aaacatttac aagacgggct
      361 gcgatcatac cgccggtgca ggactcagcc gtagccatag tcagatgctt tttctgtaaa
      421 aggtccacta cagccatttc cagagtcaca tcttcctctg tggtaaagat gcagtcacca
      481 aaacggttaa acagttctgc cattacaggc gcgatcagcg cttccccttc ttctttggta
      541 actgccttgg cagtcagacg tacctgtacc tccgcagtct tggcataggt tgcaatggtc
      601 gggttggtct gtttgtcgat cagatccagg atcttatctt ctacctggct ttccccgtat
      661 ccgcagatct ttaccatatg ggaaacaagc tgggaattct gtcttttttc cagataaggg
      721 aacacctgtc cctcaaaaag aggatacagt tcatttggcg gtcctgggag cagaatggct
      781 gtttttccat ttttttccat gatcagtccc ggtgccatac cattggggtt atccagaaca
      841 atggatccgt gaggaactgt taccattttc cagttattct ccgggatttc cctgtagata
      901 ttattcttaa aatacgcttc caggcaggct ctggtatgag ggtcctcttc cagctccatt
      961 cccatagctt ctgcacatac ttcttttgtc agatcatctt ctgtaggccc aagtcctcct
     1021 gtgacgatca caatatctga acggtcaagg gcagttttta ccaccgccat cattcggtca
     1081 taattatcgc ccactgttac ctgataatac atatccagtc ccaatgaggc acatttttct
     1141 gccagatatc tggcattgct gtttaaaatg tttcccatta agatctcagt tcccacactg
     1201 atcagttctg ctgtcatgtt ttacatgcct ccctttgtaa gcacctctac gtttttcgcc
     1261 acataatcta ccagtgaaac aatggtcagt accacagctg ccgctaacat gataaaagta
     1321 acggtcttta aagccgggat attgaatatc ataaggatca ctgcgatcat ctggaatgta
     1381 gtcttaaact tgccccagta gctggctgcg atcacaatgc cattgtctgc tgctaccaga
     1441 cggaaaccgc tgataataaa ttcacggctg ataatgatga tgactaccca tgcaggcagc
     1501 tgtcctaact ggatcaggca gatcagtgca gagcatacta acagcttatc tgctaaagga
     1561 tccataaatt ttccaaaatt tgtaacaaga ttgtacttgc gggcgatctt tccgtcaaaa
     1621 agatctgtaa ggctggcaac aataaagatc acatttgcaa caacacgcat ggtctggctg
     1681 cttccgttat catataaaag agctgctacg aaaaacggga tcaggatcac acgcataaca
     1741 gtcagtttat tcggcagatt catgatatac ctccccgatc agatcatatt cattggcacc
     1801 ggttactttt actcttacaa agtcaccgga catcagcatt tcatctgcat ttacaaaaat
     1861 aagtccgtct acgtttggcg catccatgta agtacgtcct acataagcag gctcatctgc
     1921 cacttttcct tcgatcataa catccaggac ttttcctacc atattttcac agtgttcaaa
     1981 agcgatctcc tgctgcagtt ccattacatc tgcacggcgc tcttctttca cttcttccgg
     2041 gatctggtcc ggataggaat aagccggtgt atcctcttct gcggaatagg taaatacgcc
     2101 tacacgctca aattccatct catctacaaa ttccatcagt tcttcatggt cttcttcggt
     2161 ctcccccgga aaaccgcaga taaaggttgt acgaagggca atatccggga tctcacggcg
     2221 aagggcacca atgcgctctt ttaactctgc cttgtgggtt cttcttccca tacgctttaa
     2281 aatacggtcg ctggcatgct ggatcgggat atccagatag tgacatactt tttcttctgt
     2341 tttaatagcc tggatcagct catctgtgat ctcctccgga taacagtact ggatacggat
     2401 ccagtaaatt ccagggatct gtgccagttc atgaagaagc ttcggcagtg ttttctcacc
     2461 atacagatct tttccgtaaa gagtggtctc ctgggcaact aagataagct ctcttacccc
     2521 gttttcagcc agttctcttg cttctgcaag gagctgctcc ataggaacac tgcggtaagg
     2581 tcctcttaaa tatggaatga tacagtaact gcagcgtttg tcacagcctt ccgcgatctt
     2641 taagaatgca taatgtccgc cggtagtcat tacacgcttt ttcgctgttt ccggcagttc
     2701 ttttaagtca tggaagcagg aaatatgttc ttcttttttc tctttttctt ccccaaacag
     2761 acgggtcagc acatttccga tctcttcata agaagtagtt ccaaggatgg catccacctc
     2821 cggaatctca tcaatgatct cctgtttgta gcgctgtgca agacatcccg ctacgatcag
     2881 ggcacggcat tttccttctt ctttgtaacg tgccatctct aagatcgtat taacactttc
     2941 ctcttttgca tcaccaataa agcagcaggt gttgatcagg atggcatctg cctccgtctc
     3001 atcatcggta aaggacacgc ccagtcctcc tgctacgcct aacatcttct ctgtatctac
     3061 caggttttta tcgcagccta aagatacgca aaataacttg attttttcca tgtattgtat
     3121 ttactcctga tctgtataaa ttttaaaaaa gccatccttt gcacctttac ggtacagcag
     3181 acggctttta tgaagtctta tgcctgttct tcggttgtat ccagttcttc ctcagcggat
     3241 ggctcctctt ccggaaggat ctttaccttc tgctgataca gctcatcaat ggcaatagac
     3301 agaggcttct ttccagctgc tcctgctacc attgcgtcat cacctgcgat cagctgtctt
     3361 gcacgcttgg aagctgcaat gacgatggaa taacggctct gtactaccgg ctgctcgcct
     3421 ggttcaatat ctttgtttac aacgttgatc agatctgtgt atgatggatg taacatatta
     3481 cattcccctt tcaatgtatt tattttatag tgtgatccta ttttttattt cttgatcatt
     3541 tttttgcaaa acccttatca tctaagtgat acaggtcttc tctcatcttg cttaaaaagt
     3601 ccagatgata ggaagttttt ctgtgctgca gctggatcag ctgatgcatc tcctctacgc
     3661 agcggtccag atcatcgttg atcagcaggt aatcataggc ttccataccg gaggcctctt
     3721 ctgctgctct ggccagtctg gcattgatca cttccaaagt ttctgtacct cttcctacca
     3781 ggcgcctgcg aagttcttct gcacttggag gagttacaaa taacagcagc gcatccggga
     3841 aacgtttctt tacttttaaa gctccctgga tctcgatttc aaggatcaca tccttgcctt
     3901 ctgccatttt ctgttctaca tattcctttg gtgtgccgta gtagttattc acatactggg
     3961 catactcgac cagcttccgc tcatcacgca ttttttcaaa ctgctctttt gtaacgaaaa
     4021 agtattcttt tccgtctgtt tctccctctc ttggagctct ggtggttgca gatatggata
     4081 atgcgtaatt atcataccgc tttaacagtt ctttcatcaa agttcctttg cctgcaccgg
     4141 agaaaccgga tacaacgact aaaattccct gatggttcat aaaaggcctc ctggtattat
     4201 tcaatattct ggatctgttc tcttactttt tcaatttctg tctttaaggc aatggctttg
     4261 tctgaaattg caaggtcact ggacttggaa agaatggtat ttgcttcccg gttcatctcc
     4321 tgggcaatga aatccagttt gcgacc
//
LOCUS       DBMDUA010000212         4340 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7422_length_4340_cov_2.988677, whole genome shotgun sequence.
ACCESSION   DBMDUA010000212 DBMDUA010000000
VERSION     DBMDUA010000212.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7422_length_4340_cov_2.988677"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="ACZPCL_12035"
     CDS             <1..190
                     /locus_tag="ACZPCL_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163232.1"
                     /translation="DKKRFVKSFFNLFFLVFSYFVAVHQQLSYNIMAGSICQHFFYIF
                     SKTFSGSFSDPSKHSKMP"
     gene            complement(168..869)
                     /gene="ispD"
                     /locus_tag="ACZPCL_12040"
     CDS             complement(168..869)
                     /gene="ispD"
                     /locus_tag="ACZPCL_12040"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR1163233.1"
                     /translation="MKEHCTAVVLAAGRGTRMGTQTAKQYLELQGKPILVYALEVFEQ
                     SAVVDDILLMTDKDHVEYCKKEICEKYGIKKVSAVAPGGKERYESVWKALCILKERGQ
                     SGFVMIHDGARPFITDEILKRVYEQVQVQKACVVGMPVKDTIKLINKEKQIKESPDRS
                     LVWQAQTPQAFELSLIVEAFERQLKEDCSHITDDAMVVEKQMGVPVYMVEGSYNNIKI
                     TTPEDLVIARAFLNV"
     gene            complement(893..1927)
                     /gene="tsaD"
                     /locus_tag="ACZPCL_12045"
     CDS             complement(893..1927)
                     /gene="tsaD"
                     /locus_tag="ACZPCL_12045"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR1163234.1"
                     /translation="MNTTASEDVLILAIESSCDETAASVVKNGRTVLSNVISSQIATH
                     TVYGGVVPEIASREHIKAINYVIERALTEANVTLEDITAIGVTYGPGLVGALLVGVAE
                     AKAIAYAAHKPLIGVHHIEGHVSANFIENPDLEPPFVCLIVSGGHTHLVIVKDYGEFE
                     IIGRTRDDAAGEAFDKVARAVGLGYPGGPKVDKAAKEGNPHAMEFPRAKVADSPYDFS
                     FSGLKSAVLNYINHAKMTGEEIYVPDLVASFQNAVVDVLVSRAITAAKEYGYKKLAIA
                     GGVASNSALRAAMKEACDKNGITFYHPSPIFCTDNAAMIGAAAYYEYKKGNRSGWDLN
                     AVPNLKLGER"
     gene            complement(1983..2894)
                     /locus_tag="ACZPCL_12050"
     CDS             complement(1983..2894)
                     /locus_tag="ACZPCL_12050"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity
                     producing 5'-phosphomonoesters, hydrolytic mechanism
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HIR1163235.1"
                     /translation="MLDICLLGTGGMMPLPYRWLTSMMARCAGSNLLIDCGEGTQIAL
                     KEKGWSPKPIDVICFTHYHADHISGLPGLLLTMGNAERTEPLTLIGPKGLERVVNALC
                     MIAPELPFDLKFIELTEQEESLQIGPYMIDAYRVNHKVICYGYSIRIPRKGRFDVDRA
                     REQMIPQKFWSRLQKGEIIEEDGRTFTPDMVLGADRRGLKVTYCTDTRPVPVIAQYAK
                     DADLFICEGMYGEKEKAAKAREYKHMTFYEAANLAKEAQPAQMWLTHYSPSLTRPEEY
                     MEEVKQIFPRAKAARDGWTVELEFDEE"
     gene            complement(3036..3278)
                     /locus_tag="ACZPCL_12055"
     CDS             complement(3036..3278)
                     /locus_tag="ACZPCL_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163236.1"
                     /translation="MRKIGPDGQLERMICNACGKKLAVRQGIVLEGAISIDHTWDYFS
                     EKDGQVDHFDLCEECYDQLTATFKIPVETEEQTEYL"
     gene            complement(3421..3876)
                     /gene="rimI"
                     /locus_tag="ACZPCL_12060"
     CDS             complement(3421..3876)
                     /gene="rimI"
                     /locus_tag="ACZPCL_12060"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR1163237.1"
                     /translation="MCVNEAGSIRLMEEKDVEQVEELEKQCFSVPWSENILKDGLTGR
                     FDTYLVWEKDGIVAGYCALRVLADEGEIQRIAVLPSWRRHGIAKSLMEAMIQVSKEKG
                     AVQMSLEVRESNTGARKLYENYGFIQEAVRKNYYENPVENACIMWKHEL"
     gene            complement(3913..>4340)
                     /locus_tag="ACZPCL_12065"
     CDS             complement(3913..>4340)
                     /locus_tag="ACZPCL_12065"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine biosynthesis
                     protein TsaB"
                     /protein_id="HIR1163238.1"
                     /translation="GLICPIMDAKRCQVYTGLYHMENGIETVMEQCPMDMGELIEQLN
                     KQSERVIFLGDGVPVYKAMIEEKMTVPYVFAPVQMNRQRASCVAALGMKALTEGYTGA
                     KVQPADEFVPDYLRKSQAERQREAEASPDGQAGKLPGDY"
BASE COUNT         1022 a         1135 c          799 g         1384 t
ORIGIN      
        1 agataagaaa cgttttgtca agagtttttt taatttattt tttcttgttt tttcttactt
       61 tgttgctgtt catcagcagc tcagttataa tatcatggct ggaagtatat gtcaacactt
      121 tttttatatt ttttcaaaaa ctttttcagg ttctttttca gacccttcta aacattcaaa
      181 aatgccctag caatgaccag atcttccggt gtggttatct ttatattatt atagcttccc
      241 tctaccatat aaacaggaac ccccatctgt ttttcaacta ccatggcatc atctgtgata
      301 tggctgcaat cctccttcag ctgacgttca aaggcctcta caataagaga cagctcaaaa
      361 gcctggggag tctgggcctg ccatacaaga gagcggtccg ggctttcttt gatctgcttt
      421 tctttgttga taagctttat ggtatctttt accggcatac caacaacaca ggctttttgt
      481 acctgcacct gctcgtatac ccgtttcagg atctcatctg taataaaagg tcttgcaccg
      541 tcatggatca tcacaaagcc agactgtccc cgttctttta aaatgcacag cgccttccat
      601 acactctcat agcgttcttt tccgcctgga gctacagctg acactttctt tataccatat
      661 ttctcacaga tctctttttt acagtactct acgtggtctt tatctgtcat aagcaggata
      721 tcatccacta ccgctgactg ttcaaagact tccagtgcat atacaagaat aggcttaccc
      781 tgtaattcca gatactgttt tgctgtctgt gttcccatac gggtgcctct gcctgctgca
      841 aggacgaccg ctgtgcaatg ttctttcatt ccttcttacc cctgtttccc tgttatcttt
      901 cccctaattt taaatttgga actgcattta agtcccatcc ggagcgattt ccctttttat
      961 actcatagta agccgctgca ccgatcatgg ctgcattatc ggtacaaaaa ataggagatg
     1021 gatgataaaa tgtaataccg ttcttatcgc aggcttcttt catagctgca cgaagagcgc
     1081 tgttggaagc cactccgcct gcaatagcca gcttcttata gccatattct ttagctgcag
     1141 taatagccct gcttacaagg acatctacca ctgcattctg gaaagaggct accagatccg
     1201 gcacatagat ctcttcccct gtcatttttg catggttaat atagttcagc actgcagatt
     1261 ttaagccgct gaaactgaaa tcataaggag agtctgctac ttttgcccgc ggaaattcca
     1321 tagcatgtgg atttccctct tttgctgcct tatctacctt tggaccacct ggatagccta
     1381 agcctactgc tcttgctact ttatcaaaag cttcacctgc agcatcatcc ctggtccttc
     1441 caataatctc aaactctccg taatctttta cgatcacaag atgggtatgt ccaccagata
     1501 cgatcaggca cacaaaaggc ggctccagat ccggattttc aataaaattg gcagatacat
     1561 ggccctcaat atgatgaacg ccgatcaatg gcttatgggc agcataagca atggcctttg
     1621 cctctgccac gcctactaat aatgcgccta ccaatcccgg tccataggta acaccgatag
     1681 ctgtaatgtc ctcaagagtc acatttgcct ctgtcagtgc ccgctctatt acatagttga
     1741 tcgcctttat atgctctctg gaagcgatct ccggcactac gcctccatat accgtatggg
     1801 tcgcgatctg ggatgagatc acatttgaca gtactgtacg tccatttttc acaacggaag
     1861 ctgctgtttc atcacaggag ctttcaattg ccagtattaa aacatcttct gatgcagttg
     1921 tattcatatt tccaccttat cctttatatg tgctcttgga atatgtcctc tccgggcacc
     1981 tgttactcct cgtcaaactc cagttccaca gtccagccat ctctggcagc ctttgccctt
     2041 ggaaagatct gttttacttc ctccatatat tcttccggtc ttgtaagaga cgggctgtaa
     2101 tgagtcagcc acatctgtgc aggctgagcc tcttttgcca ggtttgcagc ctcataaaag
     2161 gtcatatgct tatattccct tgcttttgct gccttttcct tttcaccgta catgccttca
     2221 cagataaaaa gatctgcgtc ttttgcatac tgtgcgatca ccggaactgg gcgtgtatct
     2281 gtacagtaag taacttttaa gcctcttctg tctgctccca gtaccatatc cggagtaaat
     2341 gtccgtccat cttcttcgat gatctcccct ttctggagac ggctccagaa tttctgaggg
     2401 atcatctgct ctcttgcacg gtctacatca aaccggcctt ttcttggaat acggatcgaa
     2461 tatccatagc agatcacctt atggttcaca cggtacgcat cgatcatgta aggaccgatc
     2521 tgtaagcttt cttcctgttc tgtcagttca ataaatttaa ggtcaaatgg aagttccgga
     2581 gcgatcatgc ataaagcatt taccacacgc tctaatccct ttggtccaat cagtgtcagc
     2641 ggttctgtgc gctctgcatt tcccatagtc agaagaagcc ctggaaggcc gctgatatgg
     2701 tctgcgtggt aatgggtaaa gcagatcaca tcaatcggct tcgggctcca tcctttttct
     2761 tttaacgcga tctgggtgcc ttctccacag tcaatgagaa gattgctgcc tgcgcacctg
     2821 gccatcatgg atgtaagcca ccggtaagga agaggcatca taccgcctgt tccaagtaaa
     2881 catatatcta acatattttt tcctcatttt ctttcattat gttcccgttg catgttctat
     2941 gcaggaacat catacattta actttccaaa tcatatcaca tttgcagctt aataatcaag
     3001 tgctgaggaa aattgagcct gtccttgtag aatggctata aatactctgt ctgttcttct
     3061 gtttctaccg gtattttaaa agtggctgtc agctgatcat agcactcttc acacaggtca
     3121 aaatgatcca cctgcccgtc tttttccgag aaataatccc atgtgtggtc aatgcttata
     3181 gctccttcta acacgatccc ctgtctgacc gccagtttct taccgcaggc attgcatatc
     3241 atcctttcca actgcccgtc tggtcctatt tttctcatat ttgtatcctc gcctttctct
     3301 gcctcttttc gtaagcggac ctgtcaccga aacgtgactt taccataaac gaaagagagg
     3361 aacgtctccc gtccctctcc attatatacg gattttattc ctgtttttac tggtagtttc
     3421 ttacaactca tgtttccaca tgatgcaggc attttctacc gggttttcat agtaattttt
     3481 gcggactgct tcctggataa aaccataatt ttcatacagt tttctggcgc ctgtattgct
     3541 ttcccgcact tccagtgaca tctgaacagc ccctttttct ttagatacct gtatcatggc
     3601 ttccatcagg cttttggcta ttccatgacg gcgccaggaa ggaaggaccg caatacgctg
     3661 gatctctccc tcatctgcaa gaactcttaa agcacagtag cctgctacga tcccgtcttt
     3721 ttcccatacc aggtaggtat caaacctgcc ggtgagcccg tcttttaata tattttcaga
     3781 ccaaggaaca gaaaagcact gtttttccag ctcttccacc tgttctacat ctttttcttc
     3841 catcaggcgg atcgatccag cctcatttac acacatttat ctctttctcc caattcttgt
     3901 aataaacagt gatcaataat ctcccggaag ttttcctgcc tgtccgtctg gtgaagcctc
     3961 tgcctcccgc tgtctttctg cctgggattt acgcaggtaa tccggtacaa attcgtctgc
     4021 aggctggact tttgctcctg tatagccttc tgtcagagct ttcataccaa gagctgccac
     4081 gcaggatgca cgctgtctgt tcatctggac aggtgcaaat acataaggaa ctgtcatttt
     4141 ctcttcgatc atggccttgt ataccggtac gccgtctccc aagaagatca cacgctcaga
     4201 ctgtttattt aactgttcga tcagttctcc catatccata gggcactgct ccataaccgt
     4261 ctcaattcca ttttccatgt ggtacagtcc tgtatatacc tggcatcttt ttgcgtccat
     4321 aatagggcag ataagaccgg
//
LOCUS       DBMDUA010000213         4266 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7570_length_4266_cov_2.356062, whole genome shotgun sequence.
ACCESSION   DBMDUA010000213 DBMDUA010000000
VERSION     DBMDUA010000213.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7570_length_4266_cov_2.356062"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(207..1475)
                     /locus_tag="ACZPCL_12070"
     CDS             complement(207..1475)
                     /locus_tag="ACZPCL_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR1163239.1"
                     /translation="MLNLKRMVCMLLTAGMFTTALPVQALAASDYQYITNISLKVEVD
                     LDAGDEVNSGDSVGTSKDDSGTKVYTTSDKYSVESAEWTNNKDVSIGDTPKITVWLEP
                     DSSSSSKYEYRFRSSYGSSKVSISGGEFVSCSKSGSSLKVVLRVNGVKGTYEAPKEAE
                     WGSGKGKATWKAPDDKTGYYDVILYKGSTVMKKLEDYNGTSYNFYPYMTKEGDYTFKV
                     RTVPHTDEQKRYGKKSEWTESDSKYIDEDDVSDGSGQDNSSNGSNSGGITPGGGTTDA
                     GWKQDNNDWYFKYPDGNYVKNNWLQWNNKWYLFDNSGKMLKGWQQKDNHWYYFDTTNG
                     GMRTGWVKEGDKWYYLNPNQGGPEGAMISNCWLTISGKTYFINQSGVMMEGWYQISGK
                     WYYFYPGDGSKAVSTSIGGFQVDADGVWNH"
     gene            complement(1668..2204)
                     /locus_tag="ACZPCL_12075"
     CDS             complement(1668..2204)
                     /locus_tag="ACZPCL_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL17 family ribosomal protein"
                     /protein_id="HIR1163240.1"
                     /translation="MAGYRKLGRTSNQRKAMIRSQVTALLYHGHIKTTETRAKEIRKV
                     AEGLIALAVKEKDNFETVKVTAKVARKDANGKRVKEVVDGKKVTVYDEVEKEIKKDSA
                     SRLHARRQMLKVLYGVTEVPTQAAGKKNGTKSVDLVAKLFDEVAPKYVSRNGGYTRIV
                     KIGQRKGDAAMEVLLELV"
     gene            complement(2373..3335)
                     /locus_tag="ACZPCL_12080"
     CDS             complement(2373..3335)
                     /locus_tag="ACZPCL_12080"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR1163241.1"
                     /translation="MFDFEKPNIEIAEISEDKRYGKFVVEPLERGYGTTLGNSLRRIM
                     LSSLPGAAVSQVKIDGVLHEFSSIPGVKEDVTEIIMNIKSLAIKNNSDTDEPKVAYIE
                     FEGEGVITAADIQADADIEIMNPDQVIATLSGGADSKFYMELTITKGRGYVSADKNKN
                     ADLPIGVIAVDSIYTPVERVNMAVANTRVGQQTDYDKLTLEIYTNGTLAPDEAVSLAA
                     KVLSEHLNLFIDLSENAKTAEIMVEKEDNEKEKVLEMNIDELELSVRSYNCLKRAGIN
                     TVEELCNRTSEDMMKVRNLGRKSLEEVLAKLKELGLQLNPSDEA"
     gene            complement(3447..4040)
                     /gene="rpsD"
                     /locus_tag="ACZPCL_12085"
     CDS             complement(3447..4040)
                     /gene="rpsD"
                     /locus_tag="ACZPCL_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR1163242.1"
                     /translation="MAVDRVPVLKRCRSLGMDPIYLGIDKKSNRESKRANKKLSEYGM
                     QLREKQKAKFIYGVLEKPFRNYYAKASKMNGMVGTNLMILLECRLDNVLFRMGFGRTR
                     KETRQIVDHKHVLVNGKQVNIPSYQVKAGDVIEIKEKFKSAQRYKDILEVTAGRMVPA
                     WLESDQENLRGTVKELPTRDEIDVPVNEMLIVELYSK"
     gene            complement(4066..>4266)
                     /gene="rpsK"
                     /locus_tag="ACZPCL_12090"
     CDS             complement(4066..>4266)
                     /gene="rpsK"
                     /locus_tag="ACZPCL_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR1163243.1"
                     /translation="QMAAETATKAALVHGLKSVDVMVKGPGSGREAAIRALQACGLEV
                     TSIKDVTPVPHNGCRPPKRRRV"
BASE COUNT         1071 a         1049 c          767 g         1379 t
ORIGIN      
        1 gatatattcg tagatcagtc tggctgtttt tataattccc atgacgttcc tttcaaagtg
       61 atgttgttga taaaaaagtc agctgctacg tgcctttggc actcccacag ctgtcacaat
      121 tgtagatgat atcatagtat aagtcaagaa aaagccacct tttcaggcag ctttttcgta
      181 tatacgtatg atttattttt tatattttaa tggttccata caccatctgc atctacctga
      241 aagcctccaa tagaagtact tacagccttg gagccatctc ccggatagaa gtaataccat
      301 ttaccggaaa tctgatacca gccttccatc atcacaccgg actggttgat gaaataagtc
      361 tttccgctga tggtcagcca gcagttggag atcatggcac cttccggacc accctggttt
      421 ggatttaagt agtaccactt gtcaccttct tttacccagc cagttctcat accgccatta
      481 gtggtgtcaa aataatacca gtgattatct ttctgctgcc agcctttcag catctttccg
      541 ctgttatcaa agagatacca tttattattc cactgcagcc agttattctt tacataattg
      601 ccatccgggt atttaaaata ccagtcgttg ttatcctgtt tccagccagc atccgtagta
      661 ccgcctccag gggtgatacc accgctgtta cttccattgg aactgttgtc ctgtccagag
      721 ccatctgata catcatcttc atcaatatac ttgctgtctg actcggtcca ttcactcttc
      781 ttaccatagc gcttctgctc atctgtatga ggaacagtac gtactttaaa ggtataatct
      841 ccttcttttg tcatataagg atagaaattg taagaagtac cattgtaatc ttccagtttc
      901 ttcataactg tggaaccctt atataagatc acatcataat aaccggtctt atcatctggt
      961 gctttccagg ttgcttttcc ttttccgctt ccccattcag cttcctttgg tgcttcataa
     1021 gtgcccttta ccccattgac acgaagaacc acttttaaac tgctgccgct cttactgcag
     1081 gatacaaatt caccgccgct gatgcttacc ttgcttgagc catagctact gcgaaatctg
     1141 tattcatact ttgagctgga tgaagagtct ggttccagcc atacagtgat ctttggagta
     1201 tctccgatag acacatcttt attattggtc cattcagcag actctacact gtatttgtct
     1261 gatgtagtat aaacctttgt tccggaatca tctttggatg ttcctacact gtcaccgctg
     1321 ttgacctcat ctcctgcatc caggtcaact tctactttca gggaaatatt cgtaatatac
     1381 tgatagtcag atgctgccag tgcctgaaca ggaagtgctg tagtaaacat ccctgctgtc
     1441 agcagcatac ataccattct ttttaaattc aacataagcc ataccctcct ccattttgta
     1501 ctttcattat aaagcaggat atgggtttta tcaacttacg atattctttc aaacctctga
     1561 cggaatacag catcgctcac aaaatccgca aaaaaaagac tgcagatacc tttgacagta
     1621 cctgcagtcg cctgcataac tatattcaga tcaatataag ttaatgatta aactaactca
     1681 agcagaactt ccatagctgc atcgccctta cgctggccga tcttaacgat tctggtgtaa
     1741 ccaccattgc ggctaacgta cttaggagca acttcatcaa ataacttagc taccagatca
     1801 acagacttgg tgccattctt ctttccagct gcctgggtag gaacctcagt tacaccgtaa
     1861 agaaccttta acatctgtct tctagcgtga agtctggaag cgctgtcctt cttgatttcc
     1921 ttctcaactt catcgtaaac agttactttc ttgccgtcta ctacttcttt aacgcgcttg
     1981 ccgtttgcat ctttacgagc aaccttagca gtaaccttta cagtctcgaa gttatctttt
     2041 tccttaacag ccagtgcgat cagaccctca gcaactttgc ggatctcttt tgctctggtt
     2101 tcagttgttt tgatgtgacc atgatataac agtgcagtta cctggcttct gatcatagcc
     2161 tttctctgat tggatgttct tcctaatttt ctatatcctg ccattattct ttcctcctga
     2221 tcggtggaac acctttccat gcatcatcgg acttactggt agaggcgcat cttacccacc
     2281 tgtttatttt ttatagtggc aacccgcgcc atgcttcatt ggacttactg gctaaggtta
     2341 tgtggggact tcccacagct ttcaatcata acttatgctt cgtcactagg attcagctgt
     2401 aagcctaatt cttttaactt ggcaagtact tcctctaagg acttacggcc aaggttacga
     2461 accttcatca tatcctcaga agttctgttg cacagttctt ctaccgtatt gattccggct
     2521 ctcttcagac aattgtaaga acgaacagaa agttccagtt cgtcgatgtt catctcaagt
     2581 actttttctt tctcattgtc ttctttctct accatgatct cagcggtctt ggcattttca
     2641 gaaaggtcaa tgaaaagatt cagatgctcg cttaatacct ttgcagcaag gctgactgct
     2701 tcatcaggag ccagggtgcc gttagtataa atttccagag tcagcttgtc atagtctgtc
     2761 tgctgtccaa cacgggtgtt ggcaacagcc atattaacac gctcaactgg agtatagata
     2821 gaatcaactg cgatcacacc aattggaaga tctgcattct tgttcttgtc agcgcttaca
     2881 tatccacggc ccttggtgat agtcagttcc atatagaact tgctgtcagc gccgccgctt
     2941 aaagttgcga ttacctgatc cggattcatg atctcgatat cagcatctgc ctgaatatca
     3001 gcagctgtaa tcacgccttc gccttcaaac tcaatgtatg caaccttcgg ttcatcggta
     3061 tcgctgttgt tcttgatcgc taagcttttg atgttcataa tgatctcagt tacatcttcc
     3121 ttaacacctg gaatagaact gaactcatgc aaaacgccat cgatttttac ctgactcact
     3181 gcggctccgg gtaaagaaga aagcataatt cttctcaggg aattgcccag tgtagtaccg
     3241 taacctcttt caagtggttc aactacaaac ttgccgtatc tcttgtcttc tgaaatctct
     3301 gcgatttcaa tgtttggttt ttcaaaatcg aacactaata gcccctcctt tttgggtttc
     3361 tatcaaatat cgagggttaa atccagcaaa gtatacctgt ccaaaataag acaggcatac
     3421 ttttcaatct ttcatatatt gatggattat ttggagtaca actcgacgat aagcatctca
     3481 tttactggaa catcaatttc atctctggtt ggcagttcct taacggtacc gcgcagattt
     3541 tcctgatcag actccagcca tgcaggaacc atacgaccag cggttacttc cagaatgtct
     3601 ttgtatctct gagcagattt gaacttctct ttgatctcga ttacatcgcc agccttaacc
     3661 tggtaagatg ggatgtttac ctgcttgcca ttaaccagaa cgtgcttgtg atcaacgatc
     3721 tgtctggttt ctttacgtgt acgtccaaat cccatacgga acagaacgtt gtccagacgg
     3781 cactcaagaa ggatcatcag gttggtacca accataccgt tcatcttgga agcctttgcg
     3841 tagtagttac ggaaaggttt ctctaatacg ccgtaaatga atttagcttt ctgcttttct
     3901 cttaactgca tgccatattc acttaacttc ttgtttgctc ttttagattc tctgttagat
     3961 tttttatcaa ttcctaaata gattggatcc ataccgaggg atctgcatct tttaagaaca
     4021 ggaactctat ctactgccac tttaattacc tcctaattaa atcaatcaga ctcttctacg
     4081 ctttggtgga cggcatccgt tgtgtggaac tggggttaca tccttgatgc tggttacttc
     4141 cagaccacat gcctgaaggg cacggattgc tgcttcacga cctgaaccag gtcctttaac
     4201 cataacgtct actgacttta agccatgtac aagagctgcc tttgtagcag tttctgctgc
     4261 catctg
//
LOCUS       DBMDUA010000214         4263 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7576_length_4263_cov_1.867612, whole genome shotgun sequence.
ACCESSION   DBMDUA010000214 DBMDUA010000000
VERSION     DBMDUA010000214.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7576_length_4263_cov_1.867612"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="ACZPCL_12095"
     CDS             <1..626
                     /locus_tag="ACZPCL_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163244.1"
                     /translation="AFGCVASTIVALLIEIGNIKEQNDKATSVYDAVYMDLKFQISWY
                     VETWARLCSVAFKDEDYRQEKHTWIEWYEITKSKFAECDDNRQAELMQFFTEQLMDSI
                     EGIEKSLKQIDSQQYILNINGIYDEGLRKILGDYSFEFYAAKLTLGREYDKADFWKSF
                     DAIKQDLINYIYNWVDIRYYNYCRFKPYKFHDDKSETMRAMLESENK"
     gene            866..1342
                     /locus_tag="ACZPCL_12100"
     CDS             866..1342
                     /locus_tag="ACZPCL_12100"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIR1163245.1"
                     /translation="MIRVLFICHGNICRSPMAEYVMKDLVKKSNLSDQFHISSAATST
                     EEIGNPVHRGTREKLAQAGIACEGHHARQMTRADYDGYDYLIGMDEWNIRNIKRITGG
                     DPEGKIFKMMSFAGSSRDVADPWYTGDFEATWKDVTEGCQGLLQYILEENGKASKK"
     gene            complement(1580..2947)
                     /locus_tag="ACZPCL_12105"
     CDS             complement(1580..2947)
                     /locus_tag="ACZPCL_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1163246.1"
                     /translation="MNLQQKKDNSFLATEPLGKLLLKLSLPTVTAQIINMLYNIVDRI
                     YIGHIPENGALALTGVGVCMPLIMIVSAFAALVGNGGAPRASIFMGKGDKESAERTLG
                     NCFTTQIIVSLLLTIILFTGNRSFLMAFGASENTIPYAVAYMNIYSLGTIFVQLTLGM
                     NAFITAQGFARTGMLSVLVGAASNIILDPVFIFVFHMGVRGAALATIISQALSCAWVL
                     NFLTGKKSYLKIRRCNMKLQKNIILPSLALGLSTFIMQASESIISVCFNSSLLKYGGD
                     LAVGAMTILTSAMQFAMLPLQGLGQGAQPIISYNFGAGQTKRVKDSFKLLLKIDLSYS
                     TFMWLLVMLFPAGFASIFTSDQALLTFTAHALRIYMASILLFGIQMACQMTFTSIGNA
                     KASIIVAVTRKFILLLPLIYIMPHIFTADQTSAVYMAEPIADFLAICFTSILFRFEFK
                     KALAR"
     gene            complement(2922..3395)
                     /locus_tag="ACZPCL_12110"
     CDS             complement(2922..3395)
                     /locus_tag="ACZPCL_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009265998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1163247.1"
                     /translation="MICITDFLAHIRLTMKLQEQMLKNICETWQLTITEGKVISFLYN
                     NPGKDTAADITELRMLSKGNVSQAVESLIQKGLLKRTADQTDRRKIHLSLLPQTADIT
                     AAICSMQANLYSQIFCGLSEEDLKFYEQINQQIMENTKKAIKERTTENEFTAKER"
     gene            3631..>4263
                     /gene="holA"
                     /locus_tag="ACZPCL_12115"
     CDS             3631..>4263
                     /gene="holA"
                     /locus_tag="ACZPCL_12115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR1163248.1"
                     /translation="MQTLNQDIKNNDFKKVYLLFGEEAFLKKSYKNRLREAITQGDTM
                     NYNYYEGKGINVNEIISLSDTMPFFADRRLILMEDSGWFKGGPGADEMCAYIENIPDT
                     TCLLFVETEVDKRSRMYKAVKKYGYIAEMVRQDSAQLGRWAAGILAKNGKKITGRTME
                     YFLGKTGDDMENITSELDKVISYTLGRDIITEEDIDAVCITQVTNKIFDMI"
BASE COUNT         1424 a          763 c          968 g         1108 t
ORIGIN      
        1 tggcatttgg gtgcgtggca tctacaattg ttgcgctgct aattgaaatt ggaaatatta
       61 aagaacaaaa cgataaagct actagtgtat atgatgcagt gtatatggat ttaaaatttc
      121 aaataagctg gtatgttgaa acttgggcac gtttgtgtag tgttgctttt aaagacgaag
      181 attatcgtca ggaaaaacat acttggattg aatggtatga gataacaaaa agcaaatttg
      241 cagaatgtga tgataacaga caagcggaat taatgcagtt ctttactgaa caactgatgg
      301 atagcataga ggggattgaa aaatccctta aacaaataga tagtcagcaa tacattttaa
      361 atattaatgg gatatatgat gagggattac gaaaaattct tggggattat agctttgaat
      421 tttatgcggc aaaattaaca ttggggagag aatatgataa agctgatttt tggaaatcct
      481 ttgatgcaat aaaacaggat ttgataaact atatatataa ttgggtagat ataagatatt
      541 acaattattg taggtttaag ccatacaaat ttcatgatga taaatcagaa acaatgcgtg
      601 caatgttgga aagtgaaaat aaataatgca gtaaagtggg atcaaattgc tacacccttt
      661 tcgttaaata atgcacaccc ccatcagtag tctgaacccc cttaactgac tacgatttga
      721 ctactacaca aggcattgaa ttgctccgtt ttgcattatt ttgcaaaaag tgtagttata
      781 aacaagaaaa aagatgatgc ccggaaaagc cgataaacca acgcaaaact cggcattaca
      841 gggtatttca gaacaggaga attttatgat tagagtactt tttatctgtc acggcaacat
      901 ttgccgctcc cccatggcag aatacgtaat gaaagatctg gtaaaaaaat ccaatctctc
      961 agaccagttc cacatctcat ccgctgccac atccacggaa gagatcggta acccggttca
     1021 tcgtggaaca cgggagaagc ttgctcaggc ggggatagct tgtgaggggc atcatgcgag
     1081 acagatgact cgggcagatt atgatgggta tgattatctt attggtatgg atgaatggaa
     1141 tatccgcaat ataaagcgga tcactggcgg agatccggag ggaaaaatat ttaagatgat
     1201 gtcctttgcc ggcagtagcc gagatgtggc agatccgtgg tatacagggg attttgaggc
     1261 tacctggaaa gatgtgacag aaggttgcca gggattgctg caatatattt tggaagaaaa
     1321 tggaaaagct agtaaaaaat aaaatagcag catattgcac atatcaataa cagaacaaaa
     1381 tatagaacaa aatatagggc aaaacacaaa acaaaagata aaacaaaaaa taccacgaaa
     1441 gataacacaa aacacaaatt caaaaactgc tgccacccag gaactaaaac ccctgggata
     1501 tggcagcagt tttctatatt aaaagaaaat atctgatcaa ataaagaatt atcaattaag
     1561 ccctgttatt acctcaaccc tatctcgcca acgccttctt aaattcaaac ctgaacaaaa
     1621 tacttgtaaa gcagatggcc agaaaatcgg caatcggttc tgccatgtaa acagcggacg
     1681 tctgatctgc agtgaaaatg tgaggcatga tgtagatcaa cgggagcagc aggataaatt
     1741 ttctggtgac agcgactatg atagatgctt ttgcgtttcc aatggaagta aaggtcatct
     1801 ggcaggccat ctggatgccg aacagcagga tggaagccat gtagatccga agtgcatgag
     1861 cagtaaaggt taaaagcgcc tggtcggagg taaagatcga agcaaaacca gccgggaaca
     1921 gcatgactaa aagccacata aaagtagaat aggaaaggtc gatctttaac agcagtttaa
     1981 aggagtcttt tacacgtttt gtctgacctg cgccaaagtt gtagctgatg atcggctggg
     2041 cgccctgtcc aagcccctgc agaggcagca tagcgaactg catggcgctt gttaaaatgg
     2101 tcatagcacc aactgcaagg tctccaccgt atttcagcag tgaagagtta aaacatacgg
     2161 aaataatgct ttcactggcc tgcatgataa aagtggaaag tcccagtgca aggcttggaa
     2221 ggataatgtt tttttgcagt ttcatattgc atctgcggat tttcagatac gatttttttc
     2281 ctgtaaggaa gtttaacacc catgcgcagg aaagtgcctg ggaaatgatc gttgcaagag
     2341 cagcaccacg aacgcccata tgaaatacga aaataaaaac aggatccagt ataatgttgc
     2401 tggcagcgcc tactaaaaca gacaacatgc cggttctggc aaatccctgt gcagtaataa
     2461 aagcgttcat acccaaagtc agctgtacaa agatagttcc cagtgaataa atattcatat
     2521 aggcaacagc gtagggaatg gtattttcac tggcaccaaa agccataaga aagcttctgt
     2581 ttccggtaaa aaggatgatg gtcagcaaaa gggatacgat gatctgtgtg gtaaaacagt
     2641 tgccaagagt gcgttcagca gactctttat ctccttttcc cataaaaata gaggctctgg
     2701 gagcgccgcc gtttcctaca agggccgcga aagcggaaac aatcatgatg aggggcatgc
     2761 acacgcctac accagtcagt gccagtgctc cgttttccgg gatatgtcca atatagatac
     2821 ggtccacaat attgtacagc atgttgatga tttgggctgt aactgtgggc agggagagct
     2881 ttagcaaaag tttccccaaa ggttctgttg caagaaagct gttatctttc ttttgctgta
     2941 aattcatttt ctgtcgtcct ttctttgatc gcttttttcg tattttccat gatctgttgg
     3001 ttgatctgtt cataaaattt caagtcttcc tcagacagac cgcaaaagat ctggctgtag
     3061 agatttgcct gcatggaaca gatggcagct gtaatgtctg cagtctgggg aagcagggat
     3121 aaatggatct ttcgacggtc agtctggtca gcggtcctct ttagcagacc tttctggatc
     3181 agactttcta cagcctggga tacatttcct ttggagagca tccgaagttc tgtgatatca
     3241 gcagcagtat cttttcctgg attattataa agaaatgaaa taacctttcc ttctgttatg
     3301 gtcagctgcc atgtttcaca gatatttttt aacatctgtt cctgcaattt catggtcagc
     3361 cggatatgtg caaggaaatc agtaatacag atcataaagg agactccttt ctgaaaaata
     3421 aaaagtttta aacaaaacag ttctaaaaaa aactatttaa tagagtacgt cagatgagaa
     3481 caaaagtcaa gccttaacat tatgcggctg gtgtgttaca atgtagccac gcggagaaat
     3541 acatgcagct gccaaacggg cagtgaaaca catccgtgtg gaacctgagt ataaggtgtc
     3601 caactaatca acaggagcaa aaagaattac atgcagaccc tgaaccagga tataaaaaat
     3661 aatgatttta agaaagtata tcttctcttt ggggaagaag catttttaaa aaaaagctat
     3721 aaaaaccgcc ttcgggaagc cattacccag ggcgatacca tgaattataa ttattatgaa
     3781 ggaaagggaa taaatgtaaa tgagatcatc agcctttccg atacaatgcc tttttttgct
     3841 gacagacgtc tgatccttat ggaggacagc ggctggttta aaggcggacc aggggcagat
     3901 gaaatgtgcg cctatataga gaatattccg gatacgacct gtcttttgtt tgtggaaacg
     3961 gaagtagata agcgaagccg tatgtataag gctgtaaaaa agtatggcta tattgcagaa
     4021 atggtgcgtc aggacagtgc ccagcttgga agatgggcag caggaatact ggcaaaaaat
     4081 ggaaaaaaga taactggccg gactatggaa tatttcctgg gaaaaacagg agatgatatg
     4141 gaaaatatta cttctgaact ggataaggtg atcagttata cacttggacg ggatattata
     4201 acggaagaag atattgatgc agtgtgtatt acccaggtga cgaacaaaat atttgacatg
     4261 atc
//
LOCUS       DBMDUA010000215         4239 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7616_length_4239_cov_2.629773, whole genome shotgun sequence.
ACCESSION   DBMDUA010000215 DBMDUA010000000
VERSION     DBMDUA010000215.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7616_length_4239_cov_2.629773"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="ACZPCL_12120"
     CDS             complement(<1..698)
                     /locus_tag="ACZPCL_12120"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1163249.1"
                     /translation="MIKLIVTDVDGTLVEDGFTNVDPRLFDTILKLREKGIQFAVASG
                     RPWASVERTFDPVKKKVFYISNNGSYIGCYGRSLYVYAMDREIVKRLIHAVRKNPEFS
                     MVYAAEDGDYTESKDDALCNWLVESYKFNLHRVDDLLTVPVPCTKLSIYRAGGVEEAV
                     KPLVEEFKDILQISCAGDVWLDCMAKNVNKGHAVKVIQDSLGIKPEETMAFGDQLNDI
                     EMLQQAYYSFAVANA"
     gene            complement(691..1323)
                     /locus_tag="ACZPCL_12125"
     CDS             complement(691..1323)
                     /locus_tag="ACZPCL_12125"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR1163250.1"
                     /translation="MELKEDLKGWQVVLASASPRRKELLEQIGICPQICPSNAEEKKD
                     ETDPQKLVEKLSSVKAEDVAGCFPAGTLVIGADTVVSIVKDGESDPVILGKPGTVEKA
                     EEMISLIQGRTHQVYTGVTVILCLGADRFAETTFAEKTDVEVYEMAEDEIRAYAASGE
                     PLDKAGAYGIQGSFAAYIKGIKGDYSNVVGLPLGRLCHEIKRLLEEREND"
     gene            complement(1325..1891)
                     /locus_tag="ACZPCL_12130"
     CDS             complement(1325..1891)
                     /locus_tag="ACZPCL_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1163251.1"
                     /translation="MSICNYINWFFLYSFLGYLLECIVLTVEYKRPVVDRGFGHGPFC
                     VIYGFGAVGACLLLSPLSDHPIELYTASMTMATTMELITANVMIRLFGSFWWDYSHKP
                     FNYRGIICLESSLAWGLLGLFFFNYLDGWVRTAVGYIPGRIGKVLAVLLTLFYVFDFI
                     RCIRMRLGIPEEGEEGETEETIGRLKVY"
     gene            complement(1935..2375)
                     /locus_tag="ACZPCL_12135"
     CDS             complement(1935..2375)
                     /locus_tag="ACZPCL_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HIR1163252.1"
                     /translation="MLGNQVLVSAVVGWTVAQVLKTLIDFALNKSFNAERLVGSGGMP
                     SSHSATVCGLTTAAALKYGAGSFEFAISFILAMVVMYDAVGVRQETGKQARLLNSILL
                     ENPLKLSSEVLQQKLKEYVGHTPIQVVAGAILGIVLALVINNCY"
     gene            complement(2397..2927)
                     /locus_tag="ACZPCL_12140"
     CDS             complement(2397..2927)
                     /locus_tag="ACZPCL_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1163253.1"
                     /translation="MDMPEANEYLMDPGYMECVADILDHPVFQSMDQYIQHGTTTCKC
                     HCIQVSYLAYKLCKRFGGNWRSAARAGLLHDLFLYDWHTHARETGDHFHGFTHPRTAM
                     ENAKQYFELTDEEKDTILRHMWPLTPVPPSTRAGYAVTFADKMCCLEETKATVKRYAA
                     VPGHLLFAQTAERGKF"
     gene            complement(2959..>4239)
                     /locus_tag="ACZPCL_12145"
     CDS             complement(2959..>4239)
                     /locus_tag="ACZPCL_12145"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033840 - alpha-1,4-glucan
                     glucosyltransferase (NDP-glucose donor) activity [Evidence
                     IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIR1163254.1"
                     /translation="KMQYVDHFYMDYLGQSRYVGILQYVHEGVTFYFIDNESYFNGPK
                     PYGDWYWDLEKFCFFCRAALSALPVIGFQPDVVHCHDWQTGLIPVLLKDSFHGGDFYR
                     NMKSVITIHNLKFQGVWDVKTIQRFTELPDYYFTPDKLEAYKDGNLLKGGIVYADAIT
                     TVSNTYAEEIKMPFYGEGLDGLMRARANSLRGIVNGIDYDDFNPETDKYIVQQYTAKN
                     FRKEKVKNKKALQQQLGLPVDEKKFMVGIVSRLTDQKGLDLVQGVMDELCSDDMQLVV
                     LGTGDERYENMFRHYDWKYHDRVSAQIYYSEEMSHRIYAGCDAFLMPSLFEPCGLSQL
                     MALRYGTVPIVRETGGLRDTVQPYNEFENTGTGFSFANYNAHEMLGSVRYAKYIYYNK
                     RREWNKIIDRDMAADFSWGTSAAKYQELYDWLIG"
BASE COUNT         1094 a         1123 c          822 g         1200 t
ORIGIN      
        1 gcatttgcca ctgcgaaact gtaataagcc tgctgcagca tttctatatc atttaactga
       61 tccccaaacg ccatggtctc ttccggcttt atccccagac tgtcctggat cacttttacc
      121 gcatgaccct tatttacatt tttagccata cagtcaagcc atacgtctcc tgcacaggag
      181 atctgaagga tgtccttaaa ttcttctacc agaggtttta ccgcttcttc cacgccgccg
      241 gctctgtata tagagagttt ggtacatgga accggcacag tcagcagatc atccacacgg
      301 tgaagattaa acttatagct ttccaccagc cagttgcaga gagcatcgtc cttggactcc
      361 gtataatctc cgtcttccgc tgcatatacc atggaaaatt ccggattttt ccgcactgca
      421 tggatcagcc gctttacgat ctcccggtcc atggcatata catacaggct tctgccatag
      481 cagccaatat aagaaccatt atttgaaata tagaacacct ttttctttac aggatcaaag
      541 gtacgttcca cactggccca gggtcttccg ctggcaactg caaactggat tcctttttcc
      601 ctcagtttca ggatcgtatc aaaaagccgt ggatctacat tggtaaaacc atcttccacc
      661 aaagttccgt ctacatctgt gacgatcagc ttaatcattt tcccgttcct ccaaaagcct
      721 ttttatctca tggcagagac gtcctaaagg aaggcctact acattgctgt aatctccttt
      781 gatccccttg atataagctg caaaggatcc ctggatccca taggcacctg ccttgtcaag
      841 aggttccccg cttgcggcat atgcccggat ctcatcttct gccatctcat agacttccac
      901 atccgtcttt tctgcaaaag tagtttctgc aaaccggtcc gctcccagac ataagatcac
      961 cgtcacaccg gtatatacct ggtgggtgcg tccctggatc agcgagatca tctcttctgc
     1021 tttttctacg gttccaggct ttcctaagat caccggatca ctttccccgt cttttacaat
     1081 agaaactacg gtatccgctc cgatcaccag ggttcccgcc ggaaaacaac cggctacatc
     1141 ttctgctttt acactggaca gtttttccac cagtttctgt gggtctgtct cgtctttctt
     1201 ttcttctgca ttgcttggac agatctgtgg gcagataccg atctgttcca acagttcttt
     1261 ccgtcttggg gaggcagatg ctaacactac ctgccaaccc tttaaatctt cttttaattc
     1321 cattttaata taccttcaat cttcctatgg tctcttctgt ttctccctct tccccttctt
     1381 ccggaattcc cagacgcatg cggatacatc tgataaagtc aaaaacataa aatagggtaa
     1441 gaaggactgc caggaccttt cctatccttc ccgggatata gcctactgct gtgcgcaccc
     1501 agccgtctaa ataattgaag aagaacaggc ctaaaagtcc ccacgccaga ctgctctcca
     1561 ggcagatgat ccctctgtag ttaaatggtt tatggctgta atcccaccag aaacttccaa
     1621 acagccggat catcacattg gctgttatca gttccatggt cgtagccata gtcatggatg
     1681 ctgtatacaa ttctatggga tgatctgaca aagggcttaa aagcaggcag gcgcccactg
     1741 ccccgaaacc gtatatgacg caaaaggggc catggccaaa gccacggtcc actaccggcc
     1801 tcttatattc aacggtgagc acaatacatt ccagaagata tcccagaaag ctgtagagaa
     1861 aaaaccagtt tatataatta caaatactca tcaattcctc cataaggaaa gtacattaca
     1921 aataaatagt tacattaata acagttattg atgaccagtg ccagaacgat acctaaaata
     1981 gcacctgcca ctacctggat cggggtatgt cccacatatt cctttaactt ctgctgaagt
     2041 acctcactgc ttaatttcag cggattttca agtaaaatgg aatttaacag tctggcctgt
     2101 ttgccagtct cctgccttac gcctaccgca tcatacataa ctaccatagc cagtataaag
     2161 gatatggcaa attcaaagga tccggcacca tattttaaag ccgccgctgt cgtcagtccg
     2221 cacacagtag ctgaatggga acttggcata cctccggaac ctaccagacg ctctgcgtta
     2281 aagcttttat tcaaagcaaa gtcgatcagg gtctttaata cctgggctac tgtccagccc
     2341 actacagcac tgacaagaac ctgatttccc agcattgctt tccatgtatc catattttaa
     2401 aattttcccc tttctgccgt ctgcgcaaac agaagatgtc ccggtacagc cgcgtaccgt
     2461 ttcacggtag cttttgtttc ttccaggcaa cacatcttat cggcaaaagt gaccgcatag
     2521 cccgctcttg ttgacggcgg gaccggtgtc agcggccaca tgtgacgcag gatcgtgtct
     2581 ttttcctcat ctgtcagttc aaaatactgt tttgcatttt ccatggctgt cctcgggtgg
     2641 gtaaagccat ggaaatgatc tcctgtttcc ctggcgtgcg tgtgccagtc gtataaaaaa
     2701 agatcatgga gaagccctgc tctcgcagcg ctcctccagt ttcccccaaa tcttttacac
     2761 aatttataag ccagataaga tacctgtata caatgacatt tacaggtcgt tgttccatgc
     2821 tgtatatact ggtccatgga ctggaataca ggatggtcta atatatctgc cacacactcc
     2881 atataccccg gatccattaa atattcattt gcttccggca tatccatgag tgtcacacct
     2941 ctttctaaac ttgccatttt agccaatgag ccagtcataa agttcctgat acttggctgc
     3001 agatgttccc caggagaaat cagctgccat atcacggtct atgatcttat tccactcacg
     3061 gcgtttgtta taataaatgt attttgcgta gcggacgctt ccaagcatct catgtgcatt
     3121 gtagtttgca aaggagaagc cggttccggt attttcaaat tcattgtaag gctgtacggt
     3181 atctcttaaa ccgccggtct ctctgacgat cggcacagta ccatagcgca gggccatcag
     3241 ctggcttaag ccacatggct caaataagga cggcatcagg aatgcgtcac atcctgcgta
     3301 gatgcggtgg gacatttcct cagagtaata gatctgtgca gatacgcggt catgatactt
     3361 ccagtcataa tgacggaaca tgttttcata ccgctcatca ccggtaccta atacaacaag
     3421 ctgcatatcg tcgctgcaaa gctcatccat cacaccctgt accaggtcaa gtcccttctg
     3481 gtctgtcaga cgggaaacga tacctaccat aaatttcttt tcatctacag gaagtcctaa
     3541 ctgctgctga agggctttct tgtttttcac tttttccttg cggaaattct tagctgtata
     3601 ctgctgtacg atatatttgt ctgtttccgg gttaaaatca tcataatcaa taccgtttac
     3661 aataccacgc aggctgtttg cccttgcacg catcaggcca tctaagcctt cgccgtaaaa
     3721 tggcatcttg atctcttctg cataggtatt acttacagta gtgatcgcgt ctgcataaac
     3781 gataccgccc tttaacaggt tgccgtcttt gtatgcttct aacttatccg gtgtgaagta
     3841 ataatccggc agttctgtaa atctctgaat cgtctttaca tcccatacgc cctggaattt
     3901 caggttgtgg atggtgatca cagacttcat attgcggtag aaatctccgc catggaaact
     3961 gtcttttaac agaaccggga tcagacctgt ctgccagtca tgacagtgta ctacatccgg
     4021 ctggaagccg atcacaggta atgcggaaag agctgcacgg cagaagaaac agaatttctc
     4081 cagatcccaa taccagtccc catatggctt tggtccgtta aaatagcttt cattgtcaat
     4141 aaagtaaaat gtaactccct catgtacata ctgaaggatg cccacataac ggctctgtcc
     4201 cagatagtcc atataaaagt ggtcaacata ctgcatctt
//
LOCUS       DBMDUA010000216         4217 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7661_length_4217_cov_3.922498, whole genome shotgun sequence.
ACCESSION   DBMDUA010000216 DBMDUA010000000
VERSION     DBMDUA010000216.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7661_length_4217_cov_3.922498"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            83..505
                     /locus_tag="ACZPCL_12150"
     CDS             83..505
                     /locus_tag="ACZPCL_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HIR1163255.1"
                     /translation="MESYTDMKYSDCGCSGRCRKVSGNRNQSVRSCLDPAMQAGSGVR
                     YPQMGNQMNDGMPQMIPETGNNWENNGNFGWNRMPGLEGNAFCPGSMPGMLEKDYPLA
                     MAYVPWQQWQTPYGPERGLAQGTIFSELDLAFDKGRCS"
     gene            506..811
                     /locus_tag="ACZPCL_12155"
     CDS             506..811
                     /locus_tag="ACZPCL_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HIR1163256.1"
                     /translation="MMGNMNNSMNGSMNRMGGTSKDALLMAVNQAGFAVTEANLYLDT
                     HPCDSQAIAYLKQMSQAYADARNAYEAQYGPLRAENARDTVYWSWTDDPWPWEGGGY"
     gene            811..1434
                     /locus_tag="ACZPCL_12160"
     CDS             811..1434
                     /locus_tag="ACZPCL_12160"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HIR1163257.1"
                     /translation="MWIYEKRLEFPVNIKEPNAKMAMFIMSQYGGPDGEMGASMRYLS
                     QRYSMPYKECKAVLTDIGTEELAHLEIVAAIVHQLTRNLTAEQLVEQGFGPYYIDHTT
                     GIWPQAAGGVPFNACEFQSKGDIITDLHEDMAAEQKARSTYDNILRVVTDYDVCEPIK
                     YLREREIVHYQRFGEALRITQEKLDSKNFYAFNPSFDNGKSVIQPRN"
     gene            complement(1514..2149)
                     /locus_tag="ACZPCL_12165"
     CDS             complement(1514..2149)
                     /locus_tag="ACZPCL_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1163258.1"
                     /translation="MEQIIIKNLSKSYGTLTVLKNVDLTLDRYKTYCLMSPSGTGKTT
                     LFRILMGLEKADEGDIIWNVGSGAGTETDTDASRSLRISAVFQEDRLCPSFSPLENVM
                     MVQKKHASEISGISRDEILTAMCRLLPEECLNRPVSTLSGGMKRRTAILRALLTRSDL
                     LLMDEPFTGLDEGTKDEVIKFLKEYRQDRFLLISTHQKEDVEKLGGILLTL"
     gene            complement(2151..2882)
                     /locus_tag="ACZPCL_12170"
     CDS             complement(2151..2882)
                     /locus_tag="ACZPCL_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1163259.1"
                     /translation="MKKYGKNWVCWLIWIGIWQLADLFIHNNIIFAGPFDMALTLAGL
                     LKDGDFWLSLFHSSLRICLGFLSAFCLGILLGSLGWAFPLVKEFLKPPMLMIRSVPVA
                     SFVILALIWIGSENLSVFISFLVVVPMIYGATLAGLENMDQKLLEMSQVFSMPFLKKV
                     RYLFIPAVHPYLVSSCRTALGMSWKSGVAAEVIGIPKASIGEQLYYTKLYLDTSGLFA
                     WTFSIILISAVFEFVFLTLLKKVRH"
     gene            complement(2927..4030)
                     /locus_tag="ACZPCL_12175"
     CDS             complement(2927..4030)
                     /locus_tag="ACZPCL_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1163260.1"
                     /translation="MKKTMKLLAAALAGTLVLGGCAGNKETTAASAESETVKETEAQS
                     TEAESSQAEAETEKEETETSDAAGSGTAIRVGSLKGPTSMGLAELMDRAEKGETENDY
                     TFTMAGKADELVGSIASGDLNIVLVPANVASILYTKTQGNVNVIDINTLGVLYVVASD
                     DSISSMEDLKGKTVYMTGKGTTPEYVMNYLLSENGLTDGDVTLEFKSEATEVASVLKE
                     DSSAVGVLPQPFATAACIQNEALKPVLDLTEQWNLLNKENGSQLVTGVTIVRSDFLKE
                     NEEAVKLFIEDHKASAAYTSEHLDEAAEMVAALGIVEKAPIAKKAMPACNIVCITGEE
                     MKSALSGYLSVLEAADAKSVGGQLPADDFYYLP"
     gene            4156..>4217
                     /locus_tag="ACZPCL_12180"
     CDS             4156..>4217
                     /locus_tag="ACZPCL_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR1163261.1"
                     /translation="MGKLYGIGVGPGDPELLTLKA"
BASE COUNT         1224 a          944 c          980 g         1069 t
ORIGIN      
        1 aagaaaggac agcagccgtt gtaacagcta ttaaccgggc tacataaatt aatagggaaa
       61 agaaaaggag ctattgattt atatggagtc atatacagat atgaaatata gtgactgcgg
      121 ctgcagtggc cggtgccgca aggtatccgg taaccggaac cagtctgtac gaagctgtct
      181 ggatccggcg atgcaggcag gaagcggagt caggtatcca cagatgggga accagatgaa
      241 tgacgggatg ccccagatga taccggaaac tggaaataat tgggaaaata acggaaattt
      301 cggctggaac cgcatgccgg ggctggaggg caatgccttc tgtccgggaa gtatgccggg
      361 gatgttagaa aaagattatc ctttggcaat ggcctatgta ccatggcagc agtggcagac
      421 gccttatgga ccagagcgtg gcttagccca aggaaccatc ttttcagagc tggacctggc
      481 ctttgataaa gggaggtgca gctagatgat gggaaacatg aataacagta tgaacggcag
      541 catgaacaga atgggtggta cttcaaaaga tgccctgctc atggctgtca atcaggcagg
      601 ttttgcggta acagaggcaa atctttatct ggatacacat ccctgcgact ctcaggcgat
      661 tgcttatctg aaacagatgt cccaggcata tgcagatgca agaaatgctt acgaagccca
      721 gtatggtccc cttcgtgctg aaaatgccag agatacagtc tactggtcct ggacagacga
      781 cccgtggcca tgggaaggag gcggttatta atgtggatct atgaaaagag actggaattt
      841 cctgtaaata ttaaagaacc aaatgcgaag atggccatgt ttatcatgag tcagtatgga
      901 ggtcccgacg gggaaatggg tgcttccatg cgttatcttt cccagcgcta ttccatgcca
      961 tacaaggagt gcaaggcagt ccttacagac attggcacgg aagagttggc ccacttggag
     1021 atcgtggcgg ccatcgtcca ccagcttacc cggaatttaa cagccgaaca gctggtagaa
     1081 cagggctttg gtccttatta tattgaccat actaccggga tctggcctca ggctgcaggc
     1141 ggtgtaccgt ttaatgcctg tgaattccag agcaagggag atattatcac cgatcttcat
     1201 gaagatatgg ctgcggaaca gaaagcccgc tccacatacg acaatatact ccgtgtagtc
     1261 acagattatg acgtatgtga gcctatcaaa tatttaagag agcgggagat cgttcattat
     1321 cagagattcg gtgaagccct gcgcatcacc caggagaagt tagacagcaa gaatttctat
     1381 gcgtttaatc cgtcttttga caatggaaaa agtgtgatcc agccgagaaa ttaaggggat
     1441 cacaaaaatc ccgcagtaaa atccagcggt atataaaaag cccggcaaaa ccgggctttt
     1501 aaacagtaaa atattacaga gtaagcaata ttccaccaag tttctccaca tcttccttct
     1561 gatgggtgga gatcagaagg aaacgatcct gtctgtattc ctttaaaaat tttatgacct
     1621 catcttttgt tccctcatcc agacctgtaa agggttcatc cattaaaagc agatccgacc
     1681 tggtaagaag agcccgcagg atggcagtcc gccgtttcat accgccgctt aaggtagata
     1741 ccggccggtt taaacattct tccggcagaa gacggcacat agctgtgagg atctcatccc
     1801 tggaaatccc ggaaatttca gaagcatgtt ttttctgcac catcatcaca ttttccaaag
     1861 gggaaaaaga aggacagagc cggtcttcct ggaaaacagc ggaaatgcgc agagaacggg
     1921 aagcatctgt atcagtttca gtcccggctc cggaccccac attccagata atatcccctt
     1981 catctgcctt ttccagtccc ataagaatac ggaataaagt ggtttttcct gttccggaag
     2041 gactcatcag gcagtaggtc ttataccggt ctaaagtcag gtccacattt tttaagacgg
     2101 tcagtgtacc ataagatttt gacaggtttt taattatgat ctgttccata tcagtgacgt
     2161 accttcttta ataatgtaag aaaaacaaac tcaaaaactg cgcttataag gataatggaa
     2221 aaggtccagg caaaaagccc ggaagtatcc agatacagtt ttgtgtaata tagttgctcg
     2281 ccaatagaag ctttcgggat accgatcact tctgcagcaa ctcctgattt ccagctcatt
     2341 cccagggcag tcctgcagct gcttaccaga taagggtgga cagccgggat gaagaggtag
     2401 cgtacttttt ttagaaatgg catggaaaag acctgggaca tttccaaaag cttttgatcc
     2461 atattttcaa gtcctgccag cgtagcgcca tagatcatgg gaactaccac cagaaaagag
     2521 ataaaaacag acagattttc tgacccgatc cagatcagtg ccagaatgac aaaggaagcc
     2581 accggtacag atcggatcat cagcataggc ggttttaaaa attctttcac caggggaaag
     2641 gcccaaccaa ggcttccaag aaggatccca aggcaaaaag cacttaaaaa tcccaggcag
     2701 atgcgaaggg aagagtgaaa caggctcagc cagaaatccc cgtcttttaa cagtcctgcc
     2761 agcgtcagtg ccatgtcaaa aggcccggca aaaataatat tgttgtggat gaaaaggtcc
     2821 gccagctgcc agatcccgat ccagatcagc cagcagaccc agttttttcc atattttttc
     2881 atcagatttt tcattgaagc aataagaaaa ggatcctgta agtgatttat ggaagatagt
     2941 agaagtcatc agccggtaac tgtccgccta cagattttgc atcagcagct tccagaacag
     3001 ataaatagcc tgagagagca gacttcattt cctcacctgt gatgcataca atgttgcagg
     3061 caggcatagc ctttttcgca atgggtgcct tttcaacgat gcccagtgca gctaccattt
     3121 cagcagcttc atccagatgt tcgcttgtat aggcagcaga tgccttgtga tcttcgatga
     3181 acagcttcac agcttcttca ttttccttta agaagtcgct gcgcacaatg gtaacacctg
     3241 taactaactg gcttccattt tccttattta aaaggttcca ctgttcagtc aggtcaagaa
     3301 ccggctttaa agcttcgttc tggatgcagg cagcagttgc aaaaggctga ggaagaacgc
     3361 ctactgcaga agaatcttct tttaatacag aagcaacctc tgtggcctca gacttaaatt
     3421 ccagagtaac atccccgtca gtaaggccat tttcagaaag gagataattc attacatatt
     3481 ccggggtagt tccttttccg gtcatgtaaa cggtctttcc tttcaggtct tccatagaag
     3541 aaatggaatc atcagaggca actacataga gaacgcccaa ggtattgatg tcgatcacat
     3601 ttacattacc ctgggtcttt gtatacagga tacttgcaac attggcaggg actaaaacaa
     3661 tattcagatc tccacttgca atggaaccta ccagttcatc tgcttttcct gccatggtaa
     3721 aggtatagtc attttctgtc tcgccttttt cagcgcgatc catcagttca gccagtccca
     3781 tagaggtagg tccttttaaa gagccaacgc ggatcgcagt acctgaaccg gctgcgtcag
     3841 aagtctcagt ttcttccttt tcagtttcag cttctgcctg tgagctttca gcctctgtgc
     3901 tctgtgcttc tgtttctttt acagtctcgc tttcagcaga agcagcagtg gtttccttat
     3961 ttcctgcaca gccgcctaac actaaagtgc ctgcaagagc agccgccagc agtttcattg
     4021 tttttttcat aataaattat tcctccctaa ataatatagt aatagaccta tcttaccata
     4081 aaaattcctt gctgtggtgg ttaagtgctg ttaaaatgga tttgttgaga aaaatagata
     4141 gaagaaaggt aaattatggg aaaattatac ggaattggag taggaccggg agatccggaa
     4201 ctgcttacat taaaagc
//
LOCUS       DBMDUA010000217         4185 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7732_length_4185_cov_1.904750, whole genome shotgun sequence.
ACCESSION   DBMDUA010000217 DBMDUA010000000
VERSION     DBMDUA010000217.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7732_length_4185_cov_1.904750"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="ACZPCL_12185"
     CDS             complement(<1..209)
                     /locus_tag="ACZPCL_12185"
                     /inference="COORDINATES: protein motif:HMM:NF014055.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HIR1163262.1"
                     /translation="MLKIESIQNGIVIDHIPAGKGMDIYKLLELDSQKQSVALLQSVR
                     SEKYGCKDLIKIEGLTEPERLEVLS"
     gene            complement(209..>1126)
                     /gene="pyrB"
                     /locus_tag="ACZPCL_12190"
     CDS             complement(209..>1126)
                     /gene="pyrB"
                     /locus_tag="ACZPCL_12190"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR1163263.1"
                     /translation="DFTLEETTKVLDLADRIAENPEVYSHVADGKKIATLFYEPSTRT
                     RLSFESAMLSLGGKALGFAGAEQSSATKGETVADTARVVSCYADIIAMRHPKEGAPLR
                     ASMYATVPVINAGDGGHNHPTQTLIDLMTIRQRKGHLDHLTVGFCGDLKFGRTVHSLV
                     NSLVRHPGNEFYFISPEELKIPDYVMEETLKPNHSFYKEVTSLEETLPKLDVLYMTRV
                     QKERFFNEEDYVRLKDTYILNREKLNLAQPDMPVLHPLPRVDEIATDVDQDPRAAYFD
                     QVQNGKYIRMALIMALLEIPDPLTGRTVL"
     assembly_gap    1127..1136
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1137..2395)
                     /locus_tag="ACZPCL_12195"
     CDS             complement(<1137..2395)
                     /locus_tag="ACZPCL_12195"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR1163264.1"
                     /translation="MLTAVTGINWGDEGKGRVIDLLAEQADVVVRYQGGNNAGHTVVT
                     DQGKFVLNLLPSGILHPGVVCVLGDGMVVDLEHLSREIHEIEKKGIRVTPDHLKLSKR
                     ATISMPWHKVQDELEENRLAKTGSAFGSTRRGIAYAYSDKYRKKTLRLGNLLHLKEDS
                     VKARLKTMLEAKNLELAGCYHQEPMSYPALLTWCEEQAELFGSYIADTGEFLEQALSE
                     GKKVVLEAQLGAMRDIDYGIFPYTSSSSTISAYGPIGAGIPGKSLDHVVGVLKAYSTC
                     VGAGPFVAEKAMSDAWEEELRKAGGEYGAATGRPRRVGPFDAVASRYGLKCQNADQIA
                     LTKMDVLSSMKEIPVITGYKRDGVVTMDFDPMDELDSFVPVVEMLPGWECDISGCRTY
                     EELPENAKAYIRILEQLLNHEIQFISVG"
     gene            2535..3428
                     /locus_tag="ACZPCL_12200"
     CDS             2535..3428
                     /locus_tag="ACZPCL_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1163265.1"
                     /translation="MDVNFEYYKIFYYVAKYHNFTKAAQALNNSQPNITRAMNCLEQQ
                     LNTTLFIRTNRGVQLTPEGERLYTHVLGAMEQFQAAEEELADSSGLSHGSIAIGASET
                     ALNIFLLDKLKNFHMTYPGIRLKLYNHSTPEAIESVKSGKIDFAVVSTPAEVDSPLKQ
                     IMLMPFQEILVGGTTFTALGSQELSLREVKNYPLISLGRETMTYKFYNSVFLSHGLDL
                     SPDTEAATADQILPLVKCELGLAFLPQPMAAPSLLKKEIVQIPLKDEIPERQICLVYD
                     SQHPMGAAARELKNTIVAGRV"
     gene            3485..3958
                     /locus_tag="ACZPCL_12205"
     CDS             3485..3958
                     /locus_tag="ACZPCL_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HIR1163266.1"
                     /translation="MDYIKEAKKLGFSNAAIMDTQKLVFKPEYRKFCEENQCGCYNVN
                     PACPPECGTVESMKQRVFAYEKALILQTIQNKDMDYKMAKLAHNKLTEQLASKIMESG
                     NTDLLIMSAGPYRHHSCMSAYCVDAQEMADAVGMICWGTDDKIRYFSQILFHGNL"
BASE COUNT         1130 a         1002 c          942 g         1101 t
ORIGIN      
        1 taactaagga cttccagacg ctcaggctct gtaagcccct ctattttaat caggtcttta
       61 cagccgtatt tttcacttct tacagactgc aaaagggcaa cggactgttt ctgggagtcc
      121 agttccagaa gcttgtaaat atccattcct tttccagccg ggatatgatc gatcacaatt
      181 ccgttttgga tagattcaat ctttaacatt acagcaccgt ccttcctgtc agcggatccg
      241 ggatttcaag cagagccatg atcaatgcca tacggatata ttttccgttc tgcacctggt
      301 caaaataagc ggctcttgga tcctggtcca cgtctgtggc aatttcgtcc acccttggga
      361 gaggatggag aaccggcata tccggctgtg ccagatttaa tttttcccgg tttaaaatat
      421 aggtatcctt taaccggaca taatcttcct cgttaaagaa acgttctttc tgaactcttg
      481 tcatatagag cacgtccagc ttgggaaggg tttcctccag gctggtgact tctttgtaaa
      541 aagaatggtt tggctttaag gtctcttcca tcacgtaatc cgggatctta agttcctccg
      601 gggagatgaa gtaaaactcg tttccaggat gacgtaccag gctgtttacc agggaatgta
      661 cggtgcgtcc aaatttcaga tccccgcaaa agccaactgt cagatgatcc agatgacctt
      721 tcctctgacg gatagtcata aggtcaatca gtgtctgggt tggatggtta tgaccgccat
      781 ccccggcatt aattactggc actgtggcat acatggaggc acgaagggga gcgccttcct
      841 tgggatgccg cattgcaatg atatctgcat agcagcttac cacccgggcg gtatcagcta
      901 ccgtttctcc ttttgtggca ctggactgct cagcaccagc aaagccaagg gcctttcctc
      961 caaggcttag catggcagat tcaaaggaaa gacgtgttct ggtacttggc tcgtaaaaaa
     1021 gagtagcaat ctttttccca tccgccacat ggctgtacac ttccggattt tctgcaatgc
     1081 ggtctgccag atccagaact tttgtagttt cttcaagggt aaaatcnnnn nnnnnnccga
     1141 cggaaataaa ctggatctca tggttcaaaa gctgctccag gatacggatg taggctttgg
     1201 cattctccgg aagctcttca tacgtccggc agccggaaat atcgcattcc cagcccggga
     1261 gcatttctac tacagggaca aagctgtcca gttcatccat agggtcaaaa tccatggtca
     1321 caacaccgtc acgtttgtag cctgtgatca cgggaatctc tttcatggaa cttaagacat
     1381 ccattttggt aagtgcgatc tgatctgcat tctggcattt taatccataa cggctggcta
     1441 cggcatcaaa cggtcccacc ctgcggggac ggcctgtggc agcaccatat tcaccgcccg
     1501 cttttcgaag ctcttcttcc caggcatcgg acattgcctt ttctgctaca aaaggaccgg
     1561 caccaacaca ggtagaatat gcttttaaaa cgcccaccac atgatccagg gattttcccg
     1621 gaattccggc accgatcggg ccataggcag aaatggtaga ggaacttgag gtatagggga
     1681 aaataccata atcaatatcc ctcatggcgc caagctgggc ttccagcact acctttttgc
     1741 cctctgacag tgcctgttcc agaaactcac ccgtgtctgc aatataggag ccaaagagtt
     1801 ctgcctgttc ctcacaccag gtgagaagag cagggtaaga cattggctcc tgatgatagc
     1861 agcctgcaag ctccaggttt tttgcctcca gcatggtttt taatctggct tttacggaat
     1921 cctcttttaa atgaagcaga ttcccaagac gaagggtttt cttacggtac ttgtcactgt
     1981 aggcgtaggc gattccacgg cgggtagagc caaaggcgct gccggttttt gccagccggt
     2041 tttcttctaa ctcatcctgt accttgtgcc atggcatgga gatcgtggca cgctttgaaa
     2101 gcttcagatg atccggtgta acccggattc cttttttctc tatttcatgg atttcccggg
     2161 aaagatgttc cagatcgact accattccgt ccccgaggac acataccaca cctggatgta
     2221 agataccaga tgggagaaga tttaagacaa atttaccctg gtcggtgacc actgtgtgac
     2281 ctgcgttgtt gccgccctgg tagcggacta ccacatctgc ctgctccgct aaaagatcga
     2341 tcacacggcc ttttccttca tcgccccagt ttataccggt tactgctgta agcatttgtt
     2401 cctcctttga aaacacgttc ctgtttccct tttttattcg ggtacataat acgcttgacg
     2461 taggaataag taaaaactat aattgatata attggaataa atatatgata tgccaaaaga
     2521 aaaggaaaaa tattatggac gtaaatttcg agtattacaa gatcttttat tatgtggcaa
     2581 aataccacaa ctttaccaag gccgcccagg cgttaaacaa cagccagcct aacatcaccc
     2641 gggccatgaa ctgtctggaa cagcagctga ataccacttt atttatccgt actaaccgcg
     2701 gtgtacagct taccccggaa ggagagcggc tttataccca tgtgcttggt gcaatggagc
     2761 agtttcaggc tgcagaagag gagctggcgg acagctccgg gctttcccac ggaagtatag
     2821 caattggcgc cagcgaaact gccttgaata ttttcctgct tgataaatta aaaaatttcc
     2881 atatgactta tccgggaatc cggctgaagc tttataacca ttccaccccg gaagccattg
     2941 aatccgtaaa aagcggaaag atcgattttg cagtagtttc cactccggca gaggtggatt
     3001 ccccattgaa acagatcatg ctcatgccct tccaggagat tctggttggt ggtaccactt
     3061 ttacagcgct tggaagccag gagctttccc ttcgggaggt aaagaactat ccgctgattt
     3121 ctcttggacg ggaaactatg acctataaat tttacaacag tgtattttta tcccatggcc
     3181 tggatctttc cccggatacg gaagctgcca ctgcggacca gatcctgccg cttgtaaaat
     3241 gcgagcttgg ccttgccttt ttgccccagc caatggcagc gccctccctt ctgaaaaaag
     3301 aaatcgtaca gatcccacta aaagacgaaa tcccggagcg tcagatctgt ctggtgtatg
     3361 acagccagca tcctatgggc gctgcggcaa gggaattaaa aaacacaatt gtagctgggc
     3421 gggtttaaaa ccagcgtatg gagaaaattt tagtgagttg taaaaaactt ggaggaggta
     3481 ttctatggat tacatcaaag aagcaaaaaa acttgggttt tcaaatgcgg caatcatgga
     3541 tacacaaaaa cttgtgttca aaccggaata ccgaaagttt tgtgaagaaa atcagtgtgg
     3601 atgttacaac gtgaatcccg cctgtccacc tgaatgcgga acagtagaat ctatgaaaca
     3661 aagggtattc gcctatgaaa aagcgcttat tctccagacg atacaaaaca aggatatgga
     3721 ctacaaaatg gcgaagcttg cgcacaataa actcaccgaa cagctggcct ctaaaataat
     3781 ggaatctgga aataccgacc tgctgatcat gtccgctggt ccttacaggc accattcctg
     3841 catgtccgcc tactgtgttg atgcccagga aatggcagat gcggtgggaa tgatctgctg
     3901 ggggaccgat gataagatac ggtatttttc acaaatttta tttcatggaa acttgtaatg
     3961 gaaaacacat acaacggtca atgatatggc agacataaaa atatgatatg aaagagaaac
     4021 ctttcatcct attgcggtat gactataaca atgctataat ccctcccgaa aagagacaag
     4081 tacagctctc atcatgatcc aaacagaaaa agaggcgttt tagtatgaaa acagttttac
     4141 caagagaaca ggctgtagaa gatatttttt ccaaaatcct ggctt
//
LOCUS       DBMDUA010000218         4151 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7788_length_4151_cov_2.248889, whole genome shotgun sequence.
ACCESSION   DBMDUA010000218 DBMDUA010000000
VERSION     DBMDUA010000218.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7788_length_4151_cov_2.248889"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(47..358)
                     /locus_tag="ACZPCL_12210"
     CDS             complement(47..358)
                     /locus_tag="ACZPCL_12210"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR1163267.1"
                     /translation="MGVLLVVMFIVFVAIGVPISFALGVVSFTGIASLGQIPNLVVFQ
                     KMFNGLNSFTLLAVPLFILAANLMNEGEITEKLIDCCNSLVGHLCGGLAYSNVLVSMI
                     F"
     gene            complement(358..843)
                     /locus_tag="ACZPCL_12215"
     CDS             complement(358..843)
                     /locus_tag="ACZPCL_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR1163268.1"
                     /translation="MKKAIEWMQKIQIAVGGFFLCIFLLTVVFQMLSRYIGIAATWTE
                     DVSMYSFIWAVFMGAGAMVYEKRHFAFTSVSDMLKNEKIKSVLAIIISLVMLVFAVLM
                     AYYGYKLTQKFWNYTWTNIPSFKRGPTWTCLPICGVTSTIYLIAQIIEEIGALMKGGN
                     K"
     gene            complement(1024..2133)
                     /locus_tag="ACZPCL_12220"
     CDS             complement(1024..2133)
                     /locus_tag="ACZPCL_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR1163269.1"
                     /translation="MKKRLALVLAGVMMVSSLAGCGSSKPAETTAAAAGETTAAAADA
                     KADTTEAAKSDAAPELELIIASNQTSLDNPYSYGMDKFKEVVEDVSGGKIAVTVHKGT
                     LGENENELIEKLEMGAASMVVASPGFMTAIGVPEVDIFSLEYLFDSFDHWEKCLDGEF
                     GDKMKEVVKEKTGNNFRIMAYWSSSVRDYYGKKPVTKPEDLKGMTIRTQSSQVQQDFW
                     KACGAIPTSVAWGELYQALQQGVVDSAENDYTSFMLKEHHKTPNGHYISETHHDYTTR
                     LLLMNGDFYDSLTDEQKGWIDQAVEACTEEERQVVYRMFKESKEKVIADGAEVTNFED
                     VDIDAFKALALPIQDKFAADNNMTAELEMIRAAAE"
     gene            complement(2544..>4151)
                     /locus_tag="ACZPCL_12225"
     CDS             complement(2544..>4151)
                     /locus_tag="ACZPCL_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1163270.1"
                     /translation="FVTPMVFHAYEAGPYTSRLMTEALEKAVKEKEIRILDKMQVIKL
                     LTFEGRIKGVLCLDKKEKEEPSYVLIWCENVILATGGPAGMYHDSVYPVSQTGSTGMA
                     FEAGAVGKNLTEWQFGMASLNPRWNVSGTYMQVLPAFISTDQEGKDEREFLLDYFKEL
                     PDLLSMVFLKGYQWPFDVNKIFGGSSVIDLLVYQETVVKGRRVFLDYRINPGKLGEKE
                     ELPYGALIPEARDYLKQAGACFGTPIERLKHMNEPAIHFYQDHHVDLYKERLEIAVCA
                     QHNNGGLSADSWWETGISGLYAVGEVCASHGVTRPGGTALNAGQVGAVRAAEGIRLKK
                     VAQTENTENDFRDADVKETLRKEAFKRIRLSENAKGDIALSALWLKASKRMSAVAGMI
                     RSRAQMEKALEETSEDVKNFEKKVCTPSVSQLPMFYKLYDMLLSQKVYLFAMLDYAKA
                     GGASRGSALYTDPEGELPGFEGCEPFGELYRCKLDRKDHGKEVQEVVLKDGEVISSRR
                     PVRPIPDVDYFFENQWRAYRKRTGTEH"
BASE COUNT         1129 a         1018 c          768 g         1236 t
ORIGIN      
        1 aaccggagaa caagggaaac acgatagagg ccattgtgtc gggcgatcag aagatcatgg
       61 atacaagtac gtttgaataa gcaagaccac cgcaaaggtg tccaactaaa gagttacagc
      121 agtcgatcag cttttcagtg atctcacctt cgttcattag gtttgctgca aggataaaca
      181 gcggaacagc taacagtgta aagctgttca gaccattaaa cattttctgg aatacaacta
      241 aattcggaat ctgtcccaga gatgcgatac cggtaaaaga tacaacgcca agtgcgaatg
      301 agatcggcac accaatggca acaaaaacga taaacattac tactaataaa acacccatta
      361 cttgttgcct cctttcataa gagcaccgat ttcttcaatg atctgcgcga tcagataaat
      421 agtagaagtt acaccacaga ttggaagaca ggtccatgta ggtccacgct taaaggacgg
      481 aatattggtc catgtatagt tccagaactt ctgtgttaat ttataaccat aataagccat
      541 taatacagca aacaccagca taacaagaga gatgatgatt gctaatacgc tcttaatttt
      601 ctcgtttttc agcatatcgc tgacggaagt aaacgcaaag tgacgttttt cataaaccat
      661 agcgcctgcg cccataaata cagcccagat aaaggaatac atagatacgt cctcagtcca
      721 ggttgctgcg atgccaatgt aacgagagag catctggaat acaactgtaa gaaggaagat
      781 gcaaaggaaa aatcctccta cagcaatctg gatcttctgc atccattcaa ttgctttttt
      841 cataatgtgt tctcctttca ggaaatctta acaccatatt aacactcttt taaacctaac
      901 atcagccatc cagtcgccca gatggctgat atcctccgga tcaggaagac ctgacccatt
      961 tcatttttct ttcgtaaatg ttcagaactt tataagtccc ttatgttccg ttctgcttac
     1021 agattactct gctgctgcac ggatcatctc taactcagca gtcatgttgt tatcagctgc
     1081 gaacttatcc tggattggaa gagccagagc cttgaatgca tcgatatcaa catcttcaaa
     1141 gttggtaact tctgcgccat cagcgataac tttctctttg gactctttga acatacggta
     1201 aactacctga cgctcttctt ctgtacaagc ctcaacagcc tgatcgatcc agcccttctg
     1261 ctcatcagtc agactatcat agaaatcgcc gttcatcagt aacagacggg ttgtgtagtc
     1321 atgatgtgtt tcagagatgt agtggccgtt tggagtcttg tgatgctctt ttaacataaa
     1381 gctggtgtag tcgttctcag cggagtcaac aacgccctgc tgtaatgcct ggtataactc
     1441 gccccatgct acagatgttg ggatagcgcc gcaagctttc cagaagtcct gctgtacctg
     1501 agaagactga gtacggattg tcataccctt taaatcttct ggcttggtaa ctggcttttt
     1561 gccgtagtaa tcacgaacag atgagctcca gtaagccata atacggaaat tgtttccggt
     1621 cttttcctta acaacttctt tcatcttgtc accgaattcg ccatctaagc acttctccca
     1681 gtgatcaaag ctgtcaaaca gatattccag tgagaagata tctacttccg gaacaccgat
     1741 agcggtcatg aaacctgggg aagcaactac catagatgca gcgcccattt ccagcttctc
     1801 gatcagctcg ttctcgttct cgcctaaggt tcccttatga acagttacag cgatctttcc
     1861 gccggaaaca tcctcaacta cctctttgaa cttgtccata ccataggaat atgggttgtc
     1921 taaagaagtc tggttggaag cgatgatcag ctctaactct ggagctgcat cagattttgc
     1981 tgcttctgtt gtgtctgctt ttgcatctgc tgctgcagcg gttgtctcgc cagcagctgc
     2041 tgctgttgtt tcagcaggtt tggatgatcc gcatccagct aatgatgata ccatcataac
     2101 acctgccagt actaatgcta accttttttt catacttcta cttccttctc tcttttattt
     2161 tcacggacac tttcatgtcc gtaaatctcg tacattcaaa gaggttttta tcccccctgc
     2221 tgactgattt tctcaataaa ctccatttga gatccgcagc acttctatgc acaattttaa
     2281 caaaaaaata acttgatttc aagttttttg tgcgattgtt ttctttgcaa tcggttgtgt
     2341 taagaagcta taaagatatt aacatatttt tttctaatat caactggttt tacacaataa
     2401 tttcgcacaa aatacacttt cgatatttgt acataatttc caaaagttgt atacaaacta
     2461 aaagctggca ggaaatatac ttacgatttt tcctgtcagc ctttttattt ttacatttta
     2521 aagtttttat agttttttta taattaatgt tcggtccctg tgcgttttct ataggcccgc
     2581 cactgatttt caaagaagta atccacatcc ggaatagggc gcaccggccg tctggaagaa
     2641 ataacctctc catcttttaa gaccacttcc tgtacttctt tcccatgatc cttccggtct
     2701 aacttacaac ggtaaagttc tccaaaaggt tcacagcctt caaatcccgg aagttcacct
     2761 tccggatctg tatataatgc agaacccctg cttgctcctc ctgcttttgc gtaatccagc
     2821 atggcaaaca gataaacttt ctgggaaagg agcatatcat acagcttgta aaacattgga
     2881 agctgtgaaa cagaaggagt acataccttc ttttcaaaat tctttacatc ttctgaagtt
     2941 tcttccaggg ctttttccat ctgtgcccgg ctgcggatca tacctgcaac tgcgctcatt
     3001 ctcttagacg cctttaacca gagagctgat aatgctatat ctccttttgc attttcagaa
     3061 agacggattc tcttaaatgc ttcttttctc agcgtttctt ttacatccgc atcccggaaa
     3121 tcgttttctg tattttctgt ctgcgccact ttcttaagcc ggatcccttc tgcagcccga
     3181 actgcaccta cctgtcctgc atttaaggcg gttccaccgg gacgggtcac gccatggctc
     3241 gcacatactt ctcctactgc atacagaccg gatattcctg tttcccacca gctgtcagct
     3301 gagagaccgc cgttattatg ctgggcgcac acggcaattt ccaaacgctc tttataaaga
     3361 tccacatgat gatcctgata gaaatggatt gcaggctcat tcatatgctt caatcgctca
     3421 atgggtgttc caaaacaggc gcctgcctgt ttcaaataat ccctggcttc cgggatcaga
     3481 gccccataag gaagctcttc cttttctccc agttttcccg gattgatcct gtaatccaaa
     3541 aagactctgc gtccttttac aacggtttcc tggtaaacta aaagatcgat cacagaagaa
     3601 cccccaaaga tcttatttac atcaaaaggc cactgatatc ctttcagaaa gaccatggaa
     3661 agcaggtccg gcagttcctt aaaataatct aaaaggaact ctctttcatc cttaccttcc
     3721 tgatctgtgg aaataaaagc cgggagtacc tgcatataag ttccgcttac attccacctt
     3781 ggatttaaag atgccattcc aaactgccat tctgtgagat tttttcccac agcgccagct
     3841 tcaaaagcca tgcctgtaga accggtctgg gaaacaggat atacactgtc atggtacatt
     3901 cccgcaggtc cccctgttgc aaggatcaca ttttcacacc agatcagcac ataagaaggt
     3961 tcttccttct cctttttatc caggcagagc actcctttta tccttccctc aaaggtaaga
     4021 agtttgatca cctgcatctt gtctaagatc cggatctctt tttcttttac tgccttttcc
     4081 agagcttctg tcatcagcct tgaagtataa ggacctgctt cgtaagcgtg gaataccatc
     4141 ggagttacaa a
//
LOCUS       DBMDUA010000219         4133 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7818_length_4133_cov_2.137897, whole genome shotgun sequence.
ACCESSION   DBMDUA010000219 DBMDUA010000000
VERSION     DBMDUA010000219.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7818_length_4133_cov_2.137897"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1965
                     /locus_tag="ACZPCL_12230"
     CDS             <1..1965
                     /locus_tag="ACZPCL_12230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR1163271.1"
                     /translation="VIDAKTQDGKEFNNQLLEECTDQLWLTGAMVLDANGNIVGEYSE
                     SGSVDRSRFAKIQEHVNKETILDIADHEEKVYSQQISCDDGGHIDVAACARRDEPGIV
                     ITYYYTSAEFLQNYKLTIQSLLNGYQTFSDGTIMIVDNGVVIASNDTSLIGQDAKKQE
                     VIQALKKNADSRHLMHLNVGDDFYYGLMLKQRDYYIYAYIPERTVFHTLPQNVIMVMF
                     IYMVVVTLVWLLLKKSRANSIKIEMEREQEYQRKLLEEAKKAEAANRAKTEFLQRMSH
                     DIRTPINGICGMVDVGDHYSNDLQKQADCRKKIREASNILLELINEVLDMSKLESGEI
                     LLEEKPFDVYKTINDVMDLVSRQADERGIKVEQTIEIEHRKLLGSSLYLKRMLMNILS
                     NAVKYNKDYGNIDLSCKEIPSHSTESTMLEFTCRDTGIGMSSGFKEHVFESFAREQKG
                     GASKFGGTGLGMTITKNLAEKMGGTITFESERDKGTTFVLRVPFKIDLDADKHKEQKN
                     VSERSIKDLNILLVEDNELNMEIAEFLLQNEGAQVTKAWNGQEAVEIFEKSRSGEFDV
                     ILMDIMMPVMNGYEAAKMIRSLDRKDAKVVPIIAMTANAFTEDRLKAKEAGMDEHIAK
                     PVDVKLLVKVISRLVENSFWGIRNEKEKME"
     gene            1943..3793
                     /locus_tag="ACZPCL_12235"
     CDS             1943..3793
                     /locus_tag="ACZPCL_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1163272.1"
                     /translation="MKKKKWNRLFSVLFVMVTVISLLSGCGRKSAEKEDAETITVYLW
                     STQLYEKYAPYIQKQLPDINIEFVVGNNDLDFYRFLKENGGLPDIITCCRFSLHDASP
                     LKDSLMDLSTTNEAGAVYNTYINSFTNQDGSVNWLPVCADAHGFVVNRDLFEKYDIPL
                     PTDYESFVSACREFKKAGIRGFAADYYYDYTCMETLQGLSASELSSAAGRKWRTIYSN
                     PENTKREGLDSTVWPEAFERMERFIKDTGLSRDDLDMNYDDVIELYKNGKVAMYFGSS
                     SGVKIFQDQGINTTFLPFFQQNGEKWLMTTPYFQVALNRDLTQDETRRQKAMKVLNTM
                     LSEDAQNQIVYEGQDILSYSQDVDIHLTEYLKDVKPVIEENHMYIRIASNDFFAISKD
                     VVSKMISGEYNAVQAYQSFNTQLLEESPISENIVLEPQKTYSNIFHTNGGNEAYSVMA
                     NTLRGIYGTDVLIASGNSFTGNVLKAKYSEKMAGRMIMPNGLWAYKCTMSGSELKETV
                     RNFVEGYEGGLIPFNRGSLPVVSGISVKVRENDDGYTLDKVTKNGKAVGDNDRFTVTC
                     LAIPRHMEADPAGKNIVFDSEDISVKDTWTGHISEGNVILAEPEDYITLR"
     gene            3841..>4133
                     /gene="mraW"
                     /locus_tag="ACZPCL_12240"
     CDS             3841..>4133
                     /gene="mraW"
                     /locus_tag="ACZPCL_12240"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HIR1163273.1"
                     /translation="MGKRLRIAVFIVFFIGIVLTAFRYFKFVSKTVYEESASHLTEVF
                     HQSNNVLNELANKNLTYLHMWGEYLKNWMRYYGVEQLDGLLADLGVSSHHFDDA"
BASE COUNT         1326 a          673 c         1045 g         1089 t
ORIGIN      
        1 gtcatagatg caaaaacaca ggacggaaaa gaatttaaca atcagctctt agaagaatgt
       61 acagaccaat tatggctgac aggtgctatg gtactggatg caaatggaaa tattgtaggt
      121 gaatatagtg agagcgggag tgtggataga agccgttttg caaaaataca ggaacatgtt
      181 aataaagaaa caatactgga tattgcagat catgaggaaa aggtatattc ccagcagatc
      241 agctgtgatg atggagggca tatagatgta gctgcatgtg ccaggagaga tgaacctggt
      301 attgtaatta cttattatta tacatctgct gagtttttgc aaaattataa gcttaccata
      361 cagagcctgt taaatggata tcagaccttt agtgacggaa cgatcatgat cgtggataat
      421 ggtgtggtca ttgcttccaa tgataccagc cttattggtc aggatgcgaa aaaacaggag
      481 gtgatccagg ctttaaagaa aaatgcagac agcagacatt taatgcattt gaatgtgggt
      541 gacgatttct attatggact gatgctgaaa caaagggatt attatattta tgcatatatt
      601 ccggagcgta ctgtgtttca tacgttgccg caaaatgtga taatggtcat gtttatttat
      661 atggtggtgg tgacactggt atggctgctt cttaaaaaat ccagggcaaa cagcattaaa
      721 atagaaatgg aaagagaaca ggagtaccag agaaaacttc tggaagaagc taaaaaggca
      781 gaggcggcca atcgggcgaa gacagaattt ctccagagga tgagtcatga tatccgtaca
      841 ccgatcaatg ggatttgcgg aatggtcgat gtaggggatc attacagcaa tgatctgcag
      901 aaacaggcag attgcaggaa aaagatccgt gaagcttcaa atattctttt agagctgatc
      961 aatgaagttc ttgatatgag caagctggaa tcaggagaaa ttttgctgga agaaaagcct
     1021 tttgatgttt ataagacaat aaatgatgtg atggacctgg ttagcagaca ggcagatgaa
     1081 aggggaataa aggttgaaca gacaatagag atcgagcacc ggaaattact gggaagctcc
     1141 ctttatctga agcgcatgct gatgaatatc ctgagtaatg ctgtgaaata caataaggat
     1201 tatggaaaca tagatctaag ctgtaaagaa atcccgtcac acagtacaga aagtacaatg
     1261 ctggaattta catgccgtga tacaggtatt ggcatgagca gcggatttaa agagcatgtt
     1321 tttgaatctt ttgccaggga gcaaaagggc ggagcttcta aatttggggg aacaggttta
     1381 gggatgacca tcaccaagaa cctggcagaa aaaatgggtg gaacgatcac ctttgaaagt
     1441 gagagggaca aaggtacgac ctttgtgctc cgggtcccat ttaaaataga cctggatgcg
     1501 gataaacata aagaacagaa gaatgtatcg gaaagatcca taaaggatct gaatatcctt
     1561 ctggtagaag ataatgagct gaatatggaa attgctgaat ttttgctcca gaatgagggc
     1621 gcccaggtga cgaaagcatg gaatggacag gaagcagttg agatattcga aaaaagcaga
     1681 tccggtgaat ttgatgtcat tcttatggat attatgatgc cggtcatgaa tggctatgaa
     1741 gcagcaaaga tgatccggtc tctggacagg aaagatgcaa aggtggtccc catcatcgca
     1801 atgacagcaa atgcctttac cgaggatagg ctgaaagcaa aagaagcagg gatggatgaa
     1861 catattgcaa aacccgttga tgtaaaatta ctggtaaaag tgatcagcag attggtggaa
     1921 aatagttttt gggggatccg taatgaaaaa gaaaaaatgg aataggttgt tttctgtact
     1981 ttttgtgatg gtgacagtta tatcacttct gtcaggctgt ggcagaaaga gtgcagaaaa
     2041 agaagatgca gaaacgatca cagtatattt gtggtccacg cagctgtatg aaaaatatgc
     2101 accatacatc cagaaacagc ttccggatat taatattgaa tttgtggtag gtaataatga
     2161 cctggatttc taccggtttc tgaaggaaaa cggtggattg ccggatatta taacctgctg
     2221 ccggttttca ctccatgatg caagcccact taaagacagc ctgatggatc tttccacaac
     2281 caatgaggca ggtgctgtct ataatacata cattaacagt tttacgaacc aggatggcag
     2341 cgtaaactgg cttcctgtgt gtgcagatgc ccatggcttt gtagtgaaca gggatctttt
     2401 tgaaaagtat gatattcctt tgccaacgga ttacgaaagc tttgtttctg cttgcaggga
     2461 atttaaaaaa gcaggtatcc gtggttttgc ggcagactat tattatgatt atacatgtat
     2521 ggaaacgctg cagggactgt ctgcatcaga gctttcttct gcagccggac gcaaatggcg
     2581 taccatctac agtaatccgg aaaatacaaa gagagaaggc ctggacagta cagtctggcc
     2641 ggaggctttt gaacgtatgg agcgattcat aaaggataca ggattgagcc gggatgatct
     2701 ggatatgaat tatgatgatg tgattgaact gtacaaaaac ggtaaggttg ccatgtattt
     2761 tggcagctct tccggtgtaa agatatttca ggatcagggc atcaacacaa catttctgcc
     2821 attttttcaa cagaacggcg aaaagtggct gatgactaca ccctatttcc aggtggcatt
     2881 aaaccgtgat ctgacacagg atgaaacacg ccggcagaag gcaatgaagg tgttgaatac
     2941 aatgctttca gaggatgctc agaaccagat cgtttatgag ggccaggata tattaagcta
     3001 cagtcaggat gtggatatcc atcttacaga atatctgaag gatgtaaagc ctgtgatcga
     3061 agaaaaccat atgtatatcc gtattgcgtc aaatgacttt tttgccattt ctaaggacgt
     3121 ggtttccaaa atgatatcag gagaatacaa tgcagtgcag gcttatcagt catttaatac
     3181 ccagcttctt gaggaaagcc ctatttctga aaatatagtg ctggagccac agaaaacata
     3241 ttccaatatc tttcatacca acggggggaa tgaagcgtat tctgttatgg caaacacttt
     3301 gcgaggtatt tatggcacag atgtgctgat cgcatcagga aacagcttta caggaaatgt
     3361 actgaaggca aaatacagtg aaaaaatggc aggcagaatg atcatgccaa atggtctttg
     3421 ggcttacaaa tgtacaatga gcgggtctga actgaaagaa acagtcagaa actttgtaga
     3481 aggctatgag ggaggtctca ttccctttaa caggggctct ctgcctgtgg tcagcggcat
     3541 ttctgtgaag gttagggaaa atgacgatgg ctatacgctg gataaagtta caaagaatgg
     3601 aaaagcagtt ggggacaatg acaggtttac tgtaacctgt ctggcaatac cgcgacacat
     3661 ggaagctgat cctgcaggta aaaatattgt atttgacagt gaagatattt ctgtgaaaga
     3721 tacctggaca ggacatattt cggagggtaa tgttattctt gcagagcctg aagactacat
     3781 tactctgagg tgagaagaat gaatattgga aatcgtaata caggcagaga gaactttttc
     3841 atgggaaaac gattaaggat agctgtcttt attgttttct tcattgggat cgttttgact
     3901 gctttccggt attttaagtt tgtgtcgaaa acggtttatg aggaaagtgc ttcccatttg
     3961 acggaggttt ttcatcagtc aaataatgtg ttaaatgaac tggcaaataa aaatctgacc
     4021 tatcttcata tgtgggggga gtatctgaag aactggatgc gttattacgg cgtagaacag
     4081 ttggacggtt tgctggctga tttgggcgtg tcgagccacc atttcgatga tgc
//
LOCUS       DBMDUA010000220         4118 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7856_length_4118_cov_3.015932, whole genome shotgun sequence.
ACCESSION   DBMDUA010000220 DBMDUA010000000
VERSION     DBMDUA010000220.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7856_length_4118_cov_3.015932"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            29..172
                     /locus_tag="ACZPCL_12245"
     CDS             29..172
                     /locus_tag="ACZPCL_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163274.1"
                     /translation="MAINKLEKIGKASEAPKAPTEKECPYCKSMIPIKAVRCPHCTSE
                     LEK"
     gene            176..667
                     /gene="nrdG"
                     /locus_tag="ACZPCL_12250"
     CDS             176..667
                     /gene="nrdG"
                     /locus_tag="ACZPCL_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIR1163275.1"
                     /translation="MRYHNITKDDMLNGEGLRTVLWVAGCSHHCPQCHNPVTWDINGG
                     LVFDEQAKEELFEELKKDYVSGVTFSGGDPFHLENREEIGALAKEIREKFPDKTIWLY
                     TGYEWEEICHLSFLSNIDVIVDGKFKIEEKDNNLHWKGSANQRVIDVKASLRAGEVRL
                     YQE"
     gene            713..1234
                     /locus_tag="ACZPCL_12255"
     CDS             713..1234
                     /locus_tag="ACZPCL_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase/dUTPase family protein"
                     /protein_id="HIR1163276.1"
                     /translation="MERIAKFHKVSYEQFEGDWKDTFGPIETEEIKRIYEGIRLPARA
                     TAGSAGYDFYTPVAVTIRPGEIVKIPTGIRAEMNEGWVLAVFPRSSLGFKYRLQLNNT
                     VGIIDSDYFFSDNEGHIFIKLTNDTKEDKTVELSEGAGFAQGIFFPFGITEDDDCSEQ
                     RNGGFGSTDSKRG"
     gene            1239..2240
                     /locus_tag="ACZPCL_12260"
     CDS             1239..2240
                     /locus_tag="ACZPCL_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024824.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1163277.1"
                     /translation="MGNRPVNRSRRSAGRGRYSSGRSRSSTLRRRRRNRRLKNVLIGL
                     CCILLVVLLVFGVGKLVERFAGPGKTQLRKEGIEKLNSGDLEGAVADFDQALEKAGNK
                     SNKASAFNADVLWYRAEAEMLLADYEAASHTYDLVAEQGGDKISSLYMKAVCAGKLED
                     KDQAVSYYREALGMEKEGVRSPGYEEALIAAGSACVKAQDSETAKSLYEEALNGGKTG
                     EKLESRIYNQLGLCQMAEEDYETAADTFDKGYNALITGYKAGTGAELDQAAAAIPKED
                     TQGLTLLKELAYNKAVCLEYRQQYRQAQAEFETYIKVFGDDEDARHEIDFLKTRQEE"
     gene            2248..3516
                     /gene="hflX"
                     /locus_tag="ACZPCL_12265"
     CDS             2248..3516
                     /gene="hflX"
                     /locus_tag="ACZPCL_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR1163278.1"
                     /translation="MIDLKELEERVILAAVSTSETDMAERSLDELKELADTAGAKTVG
                     RVIQNRESVHPGTYLGKGKLQELREMLYETGATGVICDDELSPAQLKNLEDILDTKVM
                     DRTMVILDIFAARANTREGKIQVELAQLKYRAIRLVGMRSSLSRLGGGIGTRGPGEKK
                     LETDRRLIHQRIGQLKKELEDVKRHREVTRQQRGKDHALTAAIVGYTNAGKSTLLNRL
                     TGAGILAEDKLFATLDPTTRSFTLGDGQQILLTDTVGFINKLPHHLVEAFKSTLEEAR
                     YSDIVLHVVDCSNPQMDMHMHVVKETLRELGITDKTIVTVFNKTDRLEESGLPIPRDF
                     SADYQVRISAKNGDGLKELEEILGNIIRSRRVYLEKTYSYSQAGKLQTIRKYGQLLSE
                     EYTEEGIKVTAYVPAELFAGLYGDGSEEKE"
     gene            complement(3599..3991)
                     /locus_tag="ACZPCL_12270"
     CDS             complement(3599..3991)
                     /locus_tag="ACZPCL_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR1163279.1"
                     /translation="MKEVIYTAIFVGLAILLLILCFIMFRPEKKAVPSASTYIPGKYS
                     ASLTLGNEHVNVEVTVDADQILDVSLVHLNEAVSTMYPLMQPSMNELAPQILNTQSTK
                     DLTFPESSRYTCMTLLKAVDNALEKARE"
BASE COUNT         1287 a          785 c         1102 g          944 t
ORIGIN      
        1 aaagcatagt gaaaggacgt gattccacat ggcgatcaat aaactggaaa aaattggaaa
       61 ggcttcggaa gcaccaaaag cgcctacaga gaaagaatgc ccatattgca agagcatgat
      121 tcctattaag gcagttcggt gtccacattg tacctcagaa ctggagaagt aagatatgcg
      181 ttaccataac ataacgaaag acgatatgct caatggagaa gggctgcgga ctgtgctttg
      241 ggtggcaggc tgcagccatc attgtcctca gtgccataat cctgttacat gggatattaa
      301 tggcggactg gtttttgatg agcaggcaaa agaagaatta tttgaagaac tgaaaaaaga
      361 ctatgtttcc ggtgtgactt tcagcggcgg cgatcccttt catctggaaa acagggaaga
      421 gatcggggca cttgcaaaag agatccggga aaaatttcca gataagacca tctggctgta
      481 taccggttat gaatgggaag agatctgcca tctgtcattt ctttccaata tagatgtgat
      541 cgtagacggg aaatttaaga tcgaagaaaa agacaataat cttcactgga aaggcagtgc
      601 caaccagcgt gtgatcgatg taaaagcatc ccttcgcgca ggagaggtcc gtctttacca
      661 ggagtaatga aaagaagaaa agcagcatag aaagacagga gtaaacacac acatggaaag
      721 aattgcaaaa tttcataaag taagttatga acagtttgaa ggtgactgga aagatacctt
      781 cggtcccatt gaaacagaag agatcaaaag aatttatgaa gggatccgtc tgcctgcaag
      841 ggcaactgca ggatccgcag gttatgattt ttatacaccg gtagctgtga ccatccgtcc
      901 gggtgagatc gttaagatcc ctacaggcat ccgtgcagag atgaatgagg gctgggtact
      961 tgctgtattc ccaagaagca gccttggatt taagtaccgt ctgcagttaa ataatacagt
     1021 tggcatcatt gacagtgact atttcttctc tgataatgag ggtcatattt ttatcaaact
     1081 gaccaatgat acaaaggaag acaagaccgt agaactgtca gaaggtgcag gatttgccca
     1141 gggaattttc ttcccatttg gaattacaga agatgatgac tgcagcgaac agagaaacgg
     1201 cggttttggc agtacagaca gtaaaagagg ttagtaatat gggaaacaga cctgtaaaca
     1261 gaagcaggag aagcgcaggg agaggaagat attccagtgg aaggagcaga tccagtaccc
     1321 ttagaaggcg cagaaggaac aggcgcctga aaaatgttct tattggactt tgctgtatcc
     1381 ttttagtggt ccttctggta tttggcgttg gaaaactggt ggaaagattt gccggtcccg
     1441 gaaagacaca gctgagaaaa gagggaattg aaaaattaaa ttccggtgac ttagaaggcg
     1501 ctgtggctga ttttgaccag gctcttgaaa aggcaggaaa caagtcaaat aaagcctctg
     1561 catttaatgc agatgtgttg tggtaccggg cagaagcgga aatgcttctg gcagactatg
     1621 aggcagcttc ccacacctat gacctggtag cagagcaggg cggagataag atctccagcc
     1681 tgtatatgaa ggcggtctgc gcaggaaagt tagaggacaa ggatcaggca gtttcttatt
     1741 acagagaagc actgggaatg gaaaaagaag gcgtccgttc accgggatat gaagaggcac
     1801 tgatcgctgc aggttctgcc tgcgtaaagg cacaggactc tgaaacagca aaaagtcttt
     1861 atgaagaggc attaaacggt ggaaaaacgg gagaaaaact ggaaagcagg atttataatc
     1921 agctgggtct atgccagatg gctgaagagg attatgaaac agctgctgat acgtttgaca
     1981 agggatataa tgccctgatc accggatata aagcaggaac cggggcagaa ctggatcagg
     2041 cagctgctgc gatcccgaaa gaagataccc agggacttac acttttaaaa gaactggctt
     2101 ataacaaggc agtctgcctg gaatacagac agcagtaccg tcaggcacag gcagaatttg
     2161 agacttatat caaggtattt ggggatgatg aagacgcccg gcatgagatc gactttttaa
     2221 agaccagaca ggaggaataa tacaccggtg attgatttaa aggaactgga agaacgggta
     2281 atactggcag cagtaagtac cagtgaaaca gatatggcag aacgctcttt agatgagtta
     2341 aaagaactgg cagatacagc cggggcaaag actgtaggcc gtgtgatcca gaaccgggaa
     2401 agcgttcatc cgggaaccta tctgggaaaa ggaaaactgc aggaactgcg ggagatgctt
     2461 tacgaaacag gggctacagg cgtgatctgt gacgatgaac tttcccctgc acagctcaaa
     2521 aatctggaag atatactgga tacaaaggtg atggaccgta ccatggtcat cctggatatc
     2581 tttgcagcca gagccaatac aagggaaggc aagatccagg tagaactggc ccagttaaaa
     2641 taccgtgcca tccgtctggt gggaatgcgt tcctctcttt ccagactggg aggcggtatc
     2701 ggtacaagag gacctggtga gaaaaaactg gaaacagacc gccgtcttat tcaccagcgc
     2761 attggtcagt taaagaaaga gctggaagat gtaaaacgcc accgggaagt gaccagacag
     2821 caacgtggaa aagaccatgc gttgacagct gcgatcgtag gctataccaa tgcaggaaag
     2881 tccacgttgt taaatcgtct tactggagcg gggatcctgg cagaggataa actttttgct
     2941 acgctggacc caacgacccg cagttttaca ctgggagacg gacagcagat ccttcttaca
     3001 gataccgttg gttttatcaa caaactgccc caccatctgg tagaagcatt taaaagtacc
     3061 ctggaagagg cacgctacag tgatatcgtc cttcatgtag tggactgttc caatccccag
     3121 atggatatgc atatgcatgt tgtaaaagaa actttgcggg aattggggat cacagataag
     3181 accatcgtta ctgtgtttaa taaaacagac cgtttagaag aaagcggcct gccgattcca
     3241 agggatttct ccgcagatta ccaggtgcgt atctctgcta aaaacgggga tggtttaaaa
     3301 gaattagagg agatactggg taatatcatc cgcagcagga gagtttatct ggaaaagacc
     3361 tattcttatt cccaggcagg aaagctgcag accatccgca aatatggaca gctgctttca
     3421 gaagaatata cagaagaggg gatcaaagta acggcctatg tgccggcaga actgtttgca
     3481 ggactgtatg gggatggatc ggaagaaaag gaatgatgag aaaaaggctg ccgcaagagg
     3541 aagaagatct ttgcggcggc ctttttgtag attttggata ggtttgtgcc ggtttttctt
     3601 attccctggc tttttcaagg gcattgtcta ctgcttttaa aagtgtcata caggtataac
     3661 gggaagactc agggaacgta aggtctttgg tagactgggt gtttaagatc tggggcgcaa
     3721 gttcattcat agaaggctgc attaaagggt acatggttga aactgcttcg tttaaatgga
     3781 ccaggcttac atccaggatc tggtcagcgt ctacagtgac ttccacattt acatgttcat
     3841 ttccaagagt gaggctggca ctgtatttgc cgggtatgta cgtgctggca gaaggaacgg
     3901 cctttttttc aggacggaac ataataaagc acaggatgag aagaaggatg gccagaccta
     3961 caaaaatggc agtatagatc acttctttca tccgcagcac tacgattttt gtcttggagc
     4021 ttttgaagga gactacgacc tttgggagca taaactgaaa gaaaatacga ctaaaatatt
     4081 attgaaagga gttggcggcg gctcactcat accggata
//
LOCUS       DBMDUA010000221         4111 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7869_length_4111_cov_2.993766, whole genome shotgun sequence.
ACCESSION   DBMDUA010000221 DBMDUA010000000
VERSION     DBMDUA010000221.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7869_length_4111_cov_2.993766"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            141..1361
                     /locus_tag="ACZPCL_12275"
     CDS             141..1361
                     /locus_tag="ACZPCL_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1163280.1"
                     /translation="MKKNILVGQSGGPTAVINASLYGIIKEAAAHSEQIGSVYGMING
                     IEGFLSGHIMDLSKELSDEDLELLRLTPAAYLGSCRYKLPENLDASVYTLLFEKFEQL
                     NIGAVFYIGGNDSMDTADKLSRWAAHNNKEISFIGVPKTIDNDLPVTDHTPGYGSAAR
                     YVASTVREIVLDASVYQQPAVTIVELMGRHAGWVTAASSLARKFPGDNPLLIYMPEAP
                     FEMEEFFSDLENALKQSTAVVVCVSEGIRDASGKFICEYSNEAQLDTFGHKMLTGCGK
                     LLESYVRNRFGVKVRSVELNVNQRCSSLLSSLTDINEADLAGRTAVKCALEGVTGKMI
                     TFKRADGEEYSLEYGLVDVCNVCNKEKPFPTEWITGNNNDISPEFANYALPLIKGVPE
                     HVFEDGVPKYLYRK"
     gene            complement(1614..2663)
                     /gene="uxuA"
                     /locus_tag="ACZPCL_12280"
     CDS             complement(1614..2663)
                     /gene="uxuA"
                     /locus_tag="ACZPCL_12280"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HIR1163281.1"
                     /translation="MKLSFRWYGEDDKVTLENIRQIPGMQSIVTAVYDVPVGEVWSRE
                     SIAKLKKQVEDAGLGFDVIESIPVHEDIKLGKPTRDKYIENYCENIRRVAEAGIKCIC
                     YNFMPVFDWTRTQLDHVLADGSTSLVYYQEQVDAVNPLNSDSDLTLPGWDSSYTREGL
                     KAVVAEYNNLSEEDLWNNLQYFLERVIPVAAECDVNMAIHEDDPCWSIFGLPRIITCE
                     ENLDRFLKLVDDKHNGITLCTGSLGCSGKNDVVKMAAKYAAMGRIHFAHLRNVAVLDN
                     GFEERAHLSSCGSLDMFGIVKALVDNGFDGYVRPDHGRMIWGETGRAGYGLYDRALGA
                     TYLNGLFEAVEKMSK"
     gene            complement(2924..>4111)
                     /locus_tag="ACZPCL_12285"
     CDS             complement(2924..>4111)
                     /locus_tag="ACZPCL_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163282.1"
                     /translation="SSRLGYEMCEKMVSNLTKAWVPSVFVSAVVMLLGYWTVFPHDLV
                     TNAGLIPFGSTICCYLQVAHMGTIISIKQLAEQWKVIVICMFGLVGMTVLALFLCPML
                     MDKSFVIAGLPPLTGGVVAATIMQQAAEAKGLKDAAVFAIAMYCIQGFAGYPLTAVCL
                     QLGGRKMLKEFRANKGAAYQNTGVQLDEVNGTLKAAAHKKLLPPLPAKYNSNVMIFAK
                     LALTGWIATMLAKIPVPFVGSISGLVWVLILSVILTHIGFLDEDSMNKCNSYGIVMFA
                     IMMFIYDGLKDCTMEMLGNIVVPMILLIVIGVAGMAALVFVISKVLHTGFGLSFATAL
                     TALYGFPMNAIITESTCNALAETEEEKKYLMGQMFAPMIVGGFVTVTITSVMIAGVFA
                     NML"
BASE COUNT         1158 a          996 c          807 g         1150 t
ORIGIN      
        1 ccggaaagca atgggaataa tttacaaaat aagcatctat tttctatgca ttatcacaaa
       61 tcgcattgca attagacatt attttcgcta tactgagaga agttgttgta ctacccactt
      121 acatgagagg aggatttttt ttgaaaaaaa atattcttgt aggtcagtct ggtggcccta
      181 ctgctgtaat caacgccagt ctttatggta tcattaaaga agctgccgca cattctgaac
      241 agatcggatc tgtttatggt atgatcaatg gcatcgaggg atttctctcc ggacatatta
      301 tggatctttc taaagagctt tcagatgaag acctggaact tttacgcctt acaccggcag
      361 cctaccttgg ttcctgccgc tataagctgc ctgaaaatct ggatgcttcc gtttacaccc
      421 ttctttttga aaaatttgaa cagttaaata ttggcgctgt tttctatatt ggcggaaatg
      481 actccatgga cactgctgat aagctttccc gctgggcagc tcataataat aaggaaatct
      541 cttttatcgg tgttccgaaa accatcgata atgatcttcc tgttacagac catactcccg
      601 gatacggcag cgctgcccgc tatgttgcct ctacagtccg tgagatcgta ctggacgcct
      661 ccgtttacca gcagccagct gtaaccattg tggaacttat gggacgtcat gcaggctggg
      721 tcaccgctgc cagcagtctt gcccgcaaat tcccgggaga caatccatta ctgatctata
      781 tgccggaagc tccttttgaa atggaagaat ttttctctga cctggaaaat gctttaaagc
      841 agagtaccgc tgttgttgta tgtgtctccg aaggtatccg tgacgcgtcc ggaaaattca
      901 tctgcgaata ttccaacgaa gcacagttag acacctttgg ccacaaaatg ctgaccggct
      961 gcggaaagct tcttgaaagc tatgtgcgca accgttttgg tgtaaaagtc cgctctgtag
     1021 agttaaatgt aaatcagcgc tgcagcagcc ttctttcttc ccttacagac attaacgaag
     1081 cagatctggc aggacgcact gctgttaaat gcgctttaga gggtgttaca ggtaaaatga
     1141 taacctttaa gcgtgcagat ggagaggaat acagcctgga atacggtctg gtagatgtat
     1201 gtaatgtgtg caataaagaa aaaccattcc caactgaatg gatcaccgga aacaacaatg
     1261 atatttcccc tgaatttgca aactatgcac tgccactgat caaaggtgta ccggaacatg
     1321 tatttgagga cggtgtaccg aaatatctgt acagaaagta atagaattgc gaacatttcc
     1381 ccgcttaccc agcggggatt tttgatgtaa ataagaatgt gccttggcac attctcgcgc
     1441 cgagcgcggt tgtttgcgac aaacttcctc atcgcgcgag tgcgcatcgt tggcaggtcg
     1501 tgcctttttg tgtactaggg agttccaacg gaatttccta gtacataaaa aacgcaacct
     1561 cagacagatt tttccgcctg aaactgcgtc tttaatgggt ttacgcccgt taattatttg
     1621 ctcatcttct caacagcctc aaataaacca ttcagatagg tagcgcccag tgctctgtca
     1681 tacagaccat atcctgcgcg tcctgtttca ccccagatca tacgtccgtg gtcaggtctt
     1741 acatagccgt caaagccatt gtctaccagt gccttaacga ttccgaacat atcaagggaa
     1801 ccacagctgg aaaggtgtgc tctctcttcg aaaccattat ccagaacagc tacgtttctt
     1861 aagtgtgcaa aatggatacg tcccattgct gcatacttag ctgccatctt tactacatcg
     1921 ttctttccgg agcatcctaa agaaccggtg cacagggtaa taccattgtg cttgtcatca
     1981 accagcttta agaaacgatc caggttctct tcgcaggtaa tgattcttgg aagtccgaaa
     2041 atgctccagc atggatcatc ctcatggatc gccatgttta cgtcacactc agcagctacc
     2101 gggatcactc tctccaggaa gtactgtaag ttgttccata aatcttcttc gcttaagttg
     2161 ttgtactcag caacaactgc ctttaagcct tctcttgtgt agctggaatc ccaacctgga
     2221 agagttaagt cactgtcact gtttaaagga tttacagcgt ctacctgctc ctgataataa
     2281 accagggaag tagatccgtc agccagtaca tggtctaact gggttcttgt ccagtcaaat
     2341 acaggcatga agttgtagca gatacattta ataccagctt cagcaacgcg gcggatattt
     2401 tcgcaataat tttcaatgta tttatctctg gttggcttgc ccagcttgat atcctcatga
     2461 acagggatac tctcgatcac atcaaagcca agtcctgcgt cctcaacctg cttttttaac
     2521 tttgcaatgc tctcgcggct ccatacttca cctacaggaa catcgtaaac tgcagttacg
     2581 atagactgca tacccgggat ctggcggata ttctccagag tcaccttgtc atcttctccg
     2641 taccatctga atgataattt cattgaaact tcctccttta aacattatat atttaagttt
     2701 ttatctaaat cgattgcaaa gataataccc tgatatctca cacatgtacc atctatatta
     2761 tgaattatac cagaacttcc gttttcgtcc atagtcataa ttgttgtgca tattgcaaaa
     2821 gttgttcaaa aaatcatgtg acacatgaac cgcaacataa aaaaacaggg gatagcaaac
     2881 actatcccct gaaaaaaatc ccatcaaaat cacttcattt ttattaaagc atattggcaa
     2941 atacacctgc tatcataaca gatgtgatgg taactgtaac gaacccgcct acgatcattg
     3001 gcgcaaacat ctgccccatc agatacttct tctcttcttc tgtttctgcc agagcattac
     3061 aggttgactc tgtgatgatc gcgttcatag gaaatccata aagtgctgta agtgcggttg
     3121 caaagcttaa accaaatcct gtatgtaaga cctttgaaat aacaaataca agagctgcca
     3181 ttcctgcaac accgatcacg atgagcagta tcattggtac tacgatattt cctaacattt
     3241 ccattgtaca gtctttcagt ccgtcataaa taaacatcat gatcgcaaac atcacaatac
     3301 cataggagtt gcacttattc atggaatcct cgtctaaaaa tcctatatga gtaaggataa
     3361 cggataagat cagcacccat accagtccac tgatactgcc tacaaatgga acaggtatct
     3421 tggcaagcat tgtagcgatc catccagtaa gtgccagctt tgcaaatatc attacattgc
     3481 tgttatattt cgcaggaagc ggaggaagca gctttttatg tgccgctgcc tttaatgtgc
     3541 catttacttc atccagctgc acacctgtat tctgatatgc agccccctta tttgccctga
     3601 actcctttaa catctttcgt ccaccaagct gcaggcagac cgctgtaaga ggataacctg
     3661 caaatccctg gatacagtac atggcaatag caaatacagc tgcatccttt agcccctttg
     3721 cctcagctgc ctgctgcatg atcgttgctg ccactacacc accggtcaga ggcggaagac
     3781 ctgcaataac aaaactctta tccatcagca tcgggcaaag gaataatgca agtaccgtca
     3841 ttcccaccag tccgaacata cagataacga tcactttcca ctgttcagcc agctgtttta
     3901 tactgatgat cgttcccata tgggctacct gcaggtagca gcagatagta cttccaaaag
     3961 ggatcaatcc tgcattggta acaagatcgt gtggaaatac cgtccagtat cccaaaagca
     4021 tgaccacagc gcttacaaaa acagacggaa cccaggcttt ggttaaatta gaaaccattt
     4081 tctcacacat ctcatacccc agcctggagc t
//
LOCUS       DBMDUA010000222         4095 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7905_length_4095_cov_2.143966, whole genome shotgun sequence.
ACCESSION   DBMDUA010000222 DBMDUA010000000
VERSION     DBMDUA010000222.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7905_length_4095_cov_2.143966"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(36..884)
                     /locus_tag="ACZPCL_12290"
     CDS             complement(36..884)
                     /locus_tag="ACZPCL_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1163283.1"
                     /translation="MKQSKHRTAKLVRDYLIIFVIGAVLLYPIIWMFFASFKSNDEIF
                     GSIRLLPQNFSFKYFIDGWKGSGKATYTNFFINTFLLVIPTTIFTVMSSALVAYGFAR
                     FQFPGKKILFVLLIAMLMLPNSVVIIPRYTLYNKFNWINTYMPFYAPALLCCNAFFPY
                     MLIQFLRGLPRDLDESAYIDGCGTFKTFTHILLPLMKPSLFSAGLFQFLWTYNDYFNS
                     LVFINSVKKFPISLALRMSLDAESVVQWGKVMAMAFVAVVPVILLFFAAQRYFVEGIA
                     TSGLKG"
     gene            complement(901..1788)
                     /locus_tag="ACZPCL_12295"
     CDS             complement(901..1788)
                     /locus_tag="ACZPCL_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1163284.1"
                     /translation="MNRKKTMKREYQAYLYILPWILGFAILQLYPFVSSFIYSFTDYT
                     VGAKATFQGLANYKKLFTQDKEFWNSLKVTILFALYTVPGKLIMALAVAMFLNRDLKG
                     INLIRTLYYIPSLFGGSVAVALLWRLMFLDNGVINAILSALHLPVIQWLGDTRYALRT
                     ICMLEIWQFGSSMVMFLAALKQVPRSLYEAAEIDGAGKVTRFFHITLPQISPIIFFNL
                     INQTIQALQNFTSAQVITEGGPLKSTYVLGLKLYKEGFSYFKMGYASAISWVVFAAIM
                     IFTLAIFASSKLWVHYADE"
     gene            complement(1875..3218)
                     /locus_tag="ACZPCL_12300"
     CDS             complement(1875..3218)
                     /locus_tag="ACZPCL_12300"
                     /inference="COORDINATES: protein motif:HMM:NF024808.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1163285.1"
                     /translation="MKKRLVSVALVAALAAGTLAGCGSSSSGDNTQAAAGGETAGKGG
                     SSDENITLRFAWWGGDERNEATLKVIEQFEAAHPNITIEAEYGGSDGYHDKLATQLAS
                     GTAADIVQVDPEVFPTYVSTGDYFIDYKDYDMDLSNFDENYISLEINGRYDGKQLGLP
                     TGISGSGMLVNKDLADAIGIDFSQPYTWSDLIDMGKKVREYDDSMYLLCANKEYLVNM
                     VVFNYGKQLLGKTFFDADTKTLNLTEDDLKTVYEYVKQLYDEEVVAPASYQASYTGDN
                     LQSDTNWIAGKYVAAPTYISTIDVMVAANPEANYMMGQLPVLDGATEKGWASNTPQVI
                     AITKTCEHPEAAVEFMNYFYNDDTALETLGATRSVPPTEKARKICSDKGILSEITMEG
                     ADIAAAMGGTPNDKISSSQESKTILFDSVETIGYGASTPEEAAADTINLLSGLEQ"
     gene            complement(3371..4039)
                     /locus_tag="ACZPCL_12305"
     CDS             complement(3371..4039)
                     /locus_tag="ACZPCL_12305"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1163286.1"
                     /translation="MTYNQEVAELTALQLQINPHFLFNTLQTLQFQVQSGASSEESVT
                     ILEYLSDILKYALADATETISLREELLYLKKYVAIQKFRFGEKFIIYYEVDEELMDLR
                     VFRLMLQPLVENSILHGVRYKEDRGYIKVQIFCRGGKVYFRVVDNGVGMDKAETKRLR
                     DSMKEINVHHIGLANVNSRLKLHFGDESGIRIRSKKGMGCVMEFCLPESAVRPEQTDL
                     KVEI"
     gene            4033..>4095
                     /locus_tag="ACZPCL_12310"
     CDS             4033..>4095
                     /locus_tag="ACZPCL_12310"
                     /inference="COORDINATES: protein motif:HMM:NF023074.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163287.1"
                     /translation="MSKETAEKLKPFLRRPIVYQW"
BASE COUNT         1153 a          926 c          839 g         1177 t
ORIGIN      
        1 tcatgctcat tttcttaaac tttttaacta atacatcaac cctttaaacc gctggttgca
       61 ataccttcta cgaaatatct ctgtgctgca aagaacagca ggataacagg aacaactgct
      121 acgaaagcca ttgccattac cttgccccac tgtacaacag actccgcatc cagtgacata
      181 cgaagtgcca gagagatcgg gaacttctta acagagttga taaataccag tgaattaaag
      241 taatcattgt aggtccataa gaactggaac agacctgcag agaataatga cggcttcatc
      301 agaggcagta aaatatgggt aaaggtcttg aaggttccgc agccgtcaat gtatgcggac
      361 tcatccagat ctcttggcag tcctctcaag aactggatca acatataagg gaagaaagca
      421 ttacagcata acagcgcagg tgcgtaaaat ggcatgtacg tattgatcca gttaaacttg
      481 ttgtataaag tataacgagg aatgatgact acggagtttg gcagcatcaa cattgcaata
      541 agcagtacaa acaggatctt ctttccagga aactgaaatc tggcaaatcc atatgcaacc
      601 agagcactgg acataacagt aaagatggta gtcgggatca ccagtaaaaa ggtgttgata
      661 aagaaattgg tgtaagttgc cttaccactg cccttccagc catcaataaa atatttaaag
      721 ctgaaattct gaggcagtaa acggatggaa ccaaagatct cgtcattgga cttaaaggat
      781 gcaaagaaca tccagatgat cggatacagt aaaacggcac cgatcacaaa tatgatcaga
      841 tagtcacgaa ccagtttagc ggttctgtgt tttgactgtt tcataataat ttacctcctt
      901 ttattcatcg gcataatgta cccacagctt ggaagatgcg aagatcgcca gggtaaagat
      961 catgattgct gcaaatacga cccatgaaat agcacttgcg tatcccattt taaaatagga
     1021 gaaaccttct ttgtacagtt tcagtcccag tacataagtg gactttaacg gtccgccttc
     1081 tgtaatgacc tgtgcggaag taaagttctg cagtgcctgg atggtctggt tgatcaggtt
     1141 gaagaaaatg atcggagaga tctgaggcag tgtaatatgg aagaaacgtg ttacttttcc
     1201 ggcgccgtcg atctctgctg cttcgtaaag ggagcgtgga acctgcttta atgctgccaa
     1261 gaacatgacc atggaagaac caaactgcca tatctccagc atacatatag tccgcagtgc
     1321 atagcgtgta tcacctaacc actggatcac cggcagatga agggcgctta aaatagcgtt
     1381 gatcacacca ttgtccagga acatcagtct ccataacagt gctactgcta cacttccgcc
     1441 aaaaagagac ggaatatagt ataaagtacg gatcaggttg atacctttta aatctctgtt
     1501 taagaacatg gcaactgcta aggccatgat cagttttcca ggaactgtat acagcgcaaa
     1561 aaggatcgtt acttttaaag aattccagaa ttccttatcc tgggtaaata actttttgta
     1621 gtttgcaagg ccctggaagg ttgcctttgc accaacagta taatctgtaa aagaataaat
     1681 aaaagatgaa acaaaaggat aaagctgaag aatggcaaag cctaagatcc agggaaggat
     1741 atacaggtat gcctgatatt cccttttcat ggtttttttc ctgttcataa tgacctccta
     1801 taggcagaaa aagagcgccg caaaatactt tcgtgtcctt tttgcggcac tctctgttta
     1861 tgatcaaatg cttattactg ctccagacca gacagcaggt tgatggtatc agctgcagct
     1921 tcctctgggg tgcttgcgcc gtaaccaatg gtctctactg agtcaaaaag aatggtcttg
     1981 gactcctggg aagaagaaat cttatcattt ggagttccgc ccatggctgc tgcaatatca
     2041 gcaccttcca tggtgatctc acttaaaatt cccttgtcag agcagatctt tcttgctttc
     2101 tctgttggag gaacgcttct tgttgcccct aaggtctcta atgcagtatc atcattgtag
     2161 aagtaattca taaactctac tgctgcttcc ggatgctcac aggtctttgt aatagcaatt
     2221 acctgaggag tattggatgc ccagcccttt tcagtagcgc cgtctaatac tggaagctgt
     2281 cccatcatgt agtttgcttc cggatttgct gcaaccataa catcaatagt agagatgtaa
     2341 gttggagctg ctacatactt gccggcgatc cagttggtat cagactgcag gttgtcacct
     2401 gtgtaagatg cctgatagga agcaggtgca actacttctt catcatacag ctgctttaca
     2461 tattcatata ccgtcttaag atcatcttct gttaagttca gtgtctttgt gtctgcgtca
     2521 aagaaggtct ttccaagaag ctgctttcca tagttgaata caaccatgtt tacaagatat
     2581 tctttgtttg cgcacagcag atacatgctg tcgtcatatt cgcggacttt cttacccatg
     2641 tcgatcagat cgctccaggt atatggctgg gagaaatcaa taccaatggc atctgccagg
     2701 tctttgttta ccagcatacc ggaaccggaa ataccagttg gaagtcctaa ctgctttcca
     2761 tcatagcgtc cgttgatctc cagggaaatg taattctcat cgaagtttga aagatccata
     2821 tcgtaatctt tataatcgat gaagtagtca cctgtagata cataagttgg gaacacttcc
     2881 gggtctacct gaacaatgtc tgctgctgtt ccggatgcca gctgggttgc cagtttatca
     2941 tggtaaccat cgctgccgcc gtactcagct tcaatggtga tgtttggatg agctgcctca
     3001 aactgctcga tcacctttaa ggtagcctca tttctttcat caccgcccca ccatgcaaaa
     3061 cgaagggtga tgttttcatc agagctgccg cctttgcctg ctgtctctcc accagcagca
     3121 gcctgtgtgt tgtctcctga ggatgaactt ccacatcctg ccagagtacc tgctgctaat
     3181 gcagctacca atgctacgct cacaagtctt ttcttcataa ccgaatccct ctcttttctt
     3241 tcatttgatg atcttattgt agtaaatttt tcctaaaacc gaaaggaacc ggctttttca
     3301 tttttattca ttttttaccg tttttttgca aaaggtatca tttcttttga tttttattca
     3361 ttctttgttt ttaaatttct actttcagat ctgtctgttc cggtcggaca gcactttccg
     3421 gcaggcaaaa ttccattaca cagcccattc cctttttgct gcggatccgg atcccggact
     3481 catccccaaa atgaagcttt aaacggctgt ttacatttgc aaggccgatg tgatggacat
     3541 tgatctcctt catggagtct ctgagacgtt tggtttctgc cttgtccatt cccaccccat
     3601 tgtcaaccac acggaaatag accttgccgc cgcggcagaa gatctggact ttaatataac
     3661 ccctgtcttc tttataacgc acaccatgta aaatactgtt ttctactaat ggctggagca
     3721 tcagacggaa cacccgcaga tccatcagtt cctcatctac ctcataatag ataataaatt
     3781 tctccccaaa acggaatttc tggatcgcca catatttctt taaatacaac aattcttccc
     3841 tgaggctgat cgtttctgtg gcatctgcca gtgcgtattt taaaatatcc gataaatatt
     3901 ccaggatcgt cactgactct tccgaagaag caccggactg tacctgaaat tgaagggtct
     3961 gtaaggtatt aaagagaaaa tgcgggttga tctgtaactg aagggctgtc agttctgcca
     4021 cttcctgatt atatgtcaaa agaaacggca gaaaaactta aaccgtttct ccgccgtcct
     4081 attgtttatc agtgg
//
LOCUS       DBMDUA010000223         4090 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_7917_length_4090_cov_2.353973, whole genome shotgun sequence.
ACCESSION   DBMDUA010000223 DBMDUA010000000
VERSION     DBMDUA010000223.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7917_length_4090_cov_2.353973"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="ACZPCL_12315"
     CDS             complement(<1..287)
                     /locus_tag="ACZPCL_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163288.1"
                     /translation="MDQQKITEEELNRELKGNKTTAAVCRLIGKIVAVLAFICVVTGQ
                     ILLAILLIILACVLGSVKDKKDIVLKKQIGENLVKEALQEVLEDVIYEPFG"
     gene            complement(318..1412)
                     /locus_tag="ACZPCL_12320"
     CDS             complement(318..1412)
                     /locus_tag="ACZPCL_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163289.1"
                     /translation="MRKRMSRMKKEMAVAVAAAMCISLVGCGGGSSKKVVADKKEAVE
                     TTVAETTETETTAEKLTGVNDSEMADILAGTTWAGISSTQEIMVAAFDEKDAYLAILD
                     TDGEVTDLDGYWKADTDTFYLYLNEDYSDDPTTFAFDWYNTENGEYIQLDDIVLSSEG
                     DGSNLETTLDQMMTTASVIEYVAQGTYWIGSGDETAMIFYFEDDQAYFDLLYNDNGQI
                     QTQSISGIWSLDYDHLNLIDDETGVSYELGWDLSEEGDSYCFELTEDDTTYYLYESAA
                     EDVDSTLDILTSYLTAEDSVDVESDDIDLSDFLEGYEGHSVIDAFMLSGISPDFETRK
                     YCAELLGFVNYSGTSDENLALIQLMGGTVQ"
     gene            complement(1409..2263)
                     /locus_tag="ACZPCL_12325"
     CDS             complement(1409..2263)
                     /locus_tag="ACZPCL_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163290.1"
                     /translation="MAFVKVKVDALEQQKKEYEKAIKSLDGALEQIKKVNASLGTDAM
                     LEGVRTSLTKLAVSLETRQGTLLVMIKALEQSGTKYRTAQKKAVTRSNQFRAHHRDFY
                     GNPVVVTVVAGGAGAMAGAAADAGVRPAVYTERGTSTTVSQVSERARNTAAGVPSSNR
                     SETTAGPPVTASTESIGSSDQPVSTEPVPDALGTENGSDPDFGSYIGTAGAAAAGAVI
                     GAAAGSVLGTLAGKHPQISGKAAEHKTMKPAAKEKKQEPSTDIEAQLEAARERLRRFN
                     EMEMKEKK"
     gene            complement(2273..2587)
                     /locus_tag="ACZPCL_12330"
     CDS             complement(2273..2587)
                     /locus_tag="ACZPCL_12330"
                     /inference="COORDINATES: protein motif:HMM:NF017793.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HIR1163291.1"
                     /translation="MADANIKVDPKQLKNYSNNIKTGYKNMYSYLSESKTAVKNLRST
                     WTGSGSAEFYSRFDAIIGKCEEVLNVVNTYALTLSEAADVYSANEKKISSSAGKLKIN
                     LK"
     gene            complement(2602..2904)
                     /locus_tag="ACZPCL_12335"
     CDS             complement(2602..2904)
                     /locus_tag="ACZPCL_12335"
                     /inference="COORDINATES: protein motif:HMM:NF017793.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HIR1163292.1"
                     /translation="MATQQVNAAKLKSTGVNLTNLAAKMKSEMNKLDENIQKVSSVWS
                     GEASASYLKRYQADKANLAQLTQILQQMGAALGEFSNSYTQADNKAMDLVHKYLGK"
     gene            complement(2918..3214)
                     /locus_tag="ACZPCL_12340"
     CDS             complement(2918..3214)
                     /locus_tag="ACZPCL_12340"
                     /inference="COORDINATES: protein motif:HMM:NF017793.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HIR1163293.1"
                     /translation="MAAIHFDSQSVKKQAKLLEEAADQIQNQTVKVITAANEAVAASW
                     SGKAAEIFVKFMQEQNTDLASGAASLREIAAVLRDACSSMEKAEAQAKAVVSRR"
     gene            complement(3215..3535)
                     /locus_tag="ACZPCL_12345"
     CDS             complement(3215..3535)
                     /locus_tag="ACZPCL_12345"
                     /inference="COORDINATES: protein motif:HMM:NF017793.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HIR1163294.1"
                     /translation="MAGDGTSRINTEELLRAVQEITSIKKSIAANTDATYAIFRKLQD
                     SYAGESADDIYAVAGQLRKSSGAIITMLGNYERVLKELAGVYEDTEKTVSRNAGRLKF
                     GGMR"
     gene            complement(3567..3875)
                     /locus_tag="ACZPCL_12350"
     CDS             complement(3567..3875)
                     /locus_tag="ACZPCL_12350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HIR1163295.1"
                     /translation="MAENKMEMDPEVLRTTARKVETSAGDVRKELKRFTSVIEGLSST
                     WSSEVKDRFLQNYQKDRAALLEMAEQYTEVAEGLLGIADELEQTEEEISSQIRAAAKS
                     "
BASE COUNT          894 a         1104 c          759 g         1333 t
ORIGIN      
        1 tttccgaaag gctcatagat cacatcttcc aatacctcct gaagggcttc ttttaccaga
       61 ttttcaccta tctgcttttt taggactata tcctttttat cttttacaga accaagtaca
      121 caggcaagga tgatcaacag aatggccaaa aggatctggc ctgttaccac gcatataaat
      181 gccaatactg ccacgatttt tcctatgagc ctgcatacag cagccgttgt cttatttcct
      241 ttcagttccc ggttaagctc ttcctctgtt attttctgct gatccatttt gcacctccct
      301 gctgatcctt ttttctttta ctgtaccgtg ccgcccatta actggatcag tgcaaggttt
      361 tcgtcactgg taccgctgta gtttacaaat cccaacagtt ctgcacagta ttttctagtt
      421 tcaaaatcag gagaaatacc gctgagcata aatgcgtcaa taacagaatg gccttcatat
      481 ccctctaaga aatcactgag atcaatgtca tccgactcca catctacgct gtcttctgct
      541 gtaagatagc ttgtaaggat atctaatgtg ctgtctacat cttctgctgc cgactcataa
      601 agataatagg tggtatcatc ttccgtcagc tcaaagcagt agctgtcacc ttcttctgac
      661 agatcccagc ctaattcata ggatacgcca gtttcatcat cgatcaggtt caaatggtca
      721 taatcaagac tccatatgcc ggaaatagac tgtgtctgga tctggccgtt atcattgtag
      781 agaaggtcaa aatatgcctg atcatcttca aaatagaaga tcatagctgt ttcatcaccg
      841 gaaccgatcc agtaagttcc ctgagctaca tattcgatca cgctggctgt cgtcatcatc
      901 tggtccaacg tagtttccag gttgcttccg tctccttcgc tggataagac aatatcatct
      961 aactggatat attctccgtt ttctgtgttg taccagtcaa aagcaaatgt agttggatca
     1021 tcggaatagt cttcattgag atacagataa aaggtatcgg tatctgcttt ccagtaacca
     1081 tccagatctg tcacctctcc atctgtatct aaaattgcca gataggcatc tttctcatca
     1141 aaggccgcca ccatgatctc ctgggtgctg gaaattcccg cccaggtagt tcctgccagg
     1201 atatcggcca tttctgagtc attaacacca gtaagtttct ctgctgttgt ttccgtttcc
     1261 gttgtttctg ccactgttgt ttctactgct tcttttttat cagctactac ttttttggaa
     1321 gaaccacctc cgcagccaac aagagaaata cacattgctg ctgcaactgc tactgccatt
     1381 tcttttttca tacgactcat cctctttctc attttttctc cttcatttcc atttcattaa
     1441 accgccttaa acgttctctg gctgcttcta actgcgcctc tatatctgtg gacggctcct
     1501 gttttttctc ttttgctgcc ggtttcatgg tcttatgctc cgctgctttt cctgatattt
     1561 ggggatgttt tcctgcaagc gttcccagca cacttcctgc agctgcgccg atcacagctc
     1621 ccgcagctgc ggctccagct gttcctatat atgagccaaa atctggatcc gaaccatttt
     1681 ctgttcccaa tgcatctgga actggctccg tggaaacagg ctggtctgaa gacccaatac
     1741 tttctgtgga agctgtaaca ggcggtccgg ctgttgtctc tgacctattg gaagaaggca
     1801 cacctgcggc tgtatttctt gcacgttcac ttacctgaga gactgttgta ctggtgcctc
     1861 tttctgtgta tacagcggga cggacccctg cgtctgccgc cgcacctgcc atcgccccgg
     1921 ctcctcccgc aactaccgtt acaaccaccg gatttccata aaaatccctg tggtgggcgc
     1981 ggaactgatt gcttcttgtc actgcttttt tctgagctgt cctgtatttc gtcccggact
     2041 gctctaaagc ttttatcatt acaagaagag ttccctgcct tgtttccaga gaaacagcta
     2101 actttgtaag agaagtgcgc acgccctcaa gcatggcatc tgtccccagg gaagcattta
     2161 cttttttgat ctgctccaaa gcaccatcca ggctttttat tgctttttca tactcctttt
     2221 tctgctgctc cagggcatcc accttaactt ttacgaaagc catgggcgcc tcctatttca
     2281 ggttgatctt cagtttacct gcagaactgg agatcttctt ctcattggca ctgtatacat
     2341 ctgcagcttc cgacagggtt aatgcataag tatttaccac attcaatacc tcttcacatt
     2401 tcccgataat ggcatcaaaa cggctgtaaa actccgcaga accgctgccg gtccaggtgg
     2461 atcttaaatt ttttactgca gtcttacttt ctgaaagata gctgtacatg tttttgtagc
     2521 ctgttttaat gttgttggaa tagtttttta actgtttggg atccacttta atatttgcgt
     2581 ctgccattta cgctcacctc cctatttacc cagatattta tgtaccagat ccatggcctt
     2641 gttatctgcc tgggtgtaac tgttggaaaa ttctccgaga gctgctccca tctgctgtaa
     2701 gatctgggtc agctgggcca gatttgcttt gtctgcctga tatcgtttta aatacgaagc
     2761 acttgcttcc ccgctccata cagaagatac tttttggatg ttttcatcta acttattcat
     2821 ctcacttttc atttttgctg ccagattggt gagattaact cctgtggatt taagttttgc
     2881 cgcatttacc tgctgtgttg ccataataca ctccctccta tctcctgctt accactgcct
     2941 ttgcctgcgc ttctgctttt tccatggaag aacatgcgtc ccgcaggact gccgcgatct
     3001 cccttaaaga agctgcacca gacgccaggt ctgtattctg ttcctgcata aatttcacaa
     3061 agatctctgc tgcttttcct gaccaggatg cagctactgc ttcattggcg gctgtgatca
     3121 ctttcacagt ctggttctgg atctgatcgg ccgcctcttc caacaacttt gcctgttttt
     3181 tcacactctg agagtcaaaa tgaatggctg ccatttatct catccctcca aacttaagcc
     3241 tgcctgcatt tctgcttaca gttttttctg tatcctcgta tacaccagcc agttccttaa
     3301 ggactctttc ataatttccc agcattgtga tgatcgcacc ggaacttttc cttaactgac
     3361 cggccactgc gtaaatatca tctgcgctct cccctgcata agagtcctgc agtttgcgaa
     3421 agatcgcata ggtcgcatct gtattcgctg caatggattt tttgatagaa gtgatttcct
     3481 gcactgcccg caaaagttcc tctgtattga tccgactggt tccatctcct gccatatgcc
     3541 acctccctgt aaagtctggt acagcatcag ctctttgccg ctgcacggat ctggctgctg
     3601 atctcctctt ctgtctgctc taattcatcg gcgatcccca ggagtccttc tgccacttct
     3661 gtgtactgct ctgccatttc caaaagagcc gcccggtcct tctggtaatt ttgcaaaaaa
     3721 cggtctttta cttcactgct ccaggtgctg cttaaacctt cgatcacaga tgtaaaacgc
     3781 tttaactctt ttcttacatc ccctgcagaa gtttctactt ttctggcagt ggtccgcagc
     3841 acttccggat ccatttccat tttattttct gccatgctct cctcctgttc ctgttgatgt
     3901 actcccgaac tctttaccgc atctgttttt gcagttgagc ttccatgaat acattctttg
     3961 caaccactga acgtttttat accatgacca cccggctgcc gtttttgatc cctacttccc
     4021 ggaaggtttc gttatcctgg atcagacctt ctaactccaa agatccgaaa acaatattat
     4081 ccagtcctgc
//
LOCUS       DBMDUA010000224         4038 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8023_length_4038_cov_1.676403, whole genome shotgun sequence.
ACCESSION   DBMDUA010000224 DBMDUA010000000
VERSION     DBMDUA010000224.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8023_length_4038_cov_1.676403"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="ACZPCL_12355"
     CDS             complement(<1..848)
                     /locus_tag="ACZPCL_12355"
                     /inference="COORDINATES: protein motif:HMM:NF025115.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdaC/SigV domain-containing protein"
                     /protein_id="HIR1163296.1"
                     /translation="MAAGLAVIMAFTGTGCQKILKGEPAETTSKESQEADAATKESVN
                     ESESGAEESESGPETEENAESKKTNDGMPQMLHPYIHTDSANGYADSADGSVQVSYSL
                     KTGGLVLSDEEAAAYPKLNQALALEYDTLKKSAEEDLENLKTSAEEMVEYVQEDSNMQ
                     LSAVYTPDILRADENVVSYELFYYDYYGGAHGYNSYTGLTFDTKTGKKLGFYDVITDE
                     DKVKSGIVHELKDKYASEEGLVENSTPEEDADVFFECINTPQDNGAVAWSLGTDRLNI
                     YYNPYT"
     assembly_gap    903..912
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            982..1053
                     /locus_tag="ACZPCL_12360"
     tRNA            982..1053
                     /locus_tag="ACZPCL_12360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1015..1017,aa:Arg,seq:ccg)
     gene            1314..2228
                     /locus_tag="ACZPCL_12365"
     CDS             1314..2228
                     /locus_tag="ACZPCL_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5688 family protein"
                     /protein_id="HIR1163297.1"
                     /translation="MVYDTFLNTVKEQMEQALGDGYSLTLRKVQKNNGLILDGLCIAK
                     GNGTIAPSIYLNSCYEQYLNGTSMEEIIQKLLTAYYESSWPPILNKLVLSDYDSLRSH
                     IAFRLINASSNQSLLKTMPHIPWMDLAIVFYLYIQENENGIMTAAISDHHMKTWGVTV
                     HDLYHDSLLNTPRLFPPVISSMACILESLDPKHLQHQPAFPETPFYVLTNQNGINGAA
                     CILYKDVIKNFAEGMDRDILILPSSIHEVLLLPDDDPLSYDELGHLVTHINQTEVVAE
                     DRLSNQVYLYSRKEDRITTVSFGPSAVC"
     gene            complement(2232..3413)
                     /locus_tag="ACZPCL_12370"
     CDS             complement(2232..3413)
                     /locus_tag="ACZPCL_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR1163298.1"
                     /translation="MKMKKAWCIALAAAMTFMSVMPAFAADTKIDTVKLTFSYDTAPA
                     SGDSIGSIHVSGDTSSYYVESAEYTNADDQDTWTVGDIPEVKVELYAKDGYRFSYTSK
                     SHFKLSGCNAEFKKAKIYDSGDYIEVTAQLKRIGGKLEGTSNLEWNDGIAEWDSIDGA
                     KSYDVKLLRDEKTVTTVNTTGTTFNFNGYFNKEGDYTFRVRAIASYNDKAGEWSDDSS
                     SYYVDEDEAGVYNGIGRWVQDNVGWWYSYSSGGYPSNGWKQINGAWYYFNTSGYRQTG
                     WVRVDRQWYYLNGDGVMTTGWQLVGGQWYYMNGSGEMQTGWQAVGGKWYYLDPTGAMK
                     TGWQQLGGQWYYLNGSGEMTTGWQAVGGRWYYMGSNGVMYANTWTPDGRYVDGSGAWV
                     Q"
     gene            complement(3573..>4038)
                     /locus_tag="ACZPCL_12375"
     CDS             complement(3573..>4038)
                     /locus_tag="ACZPCL_12375"
                     /inference="COORDINATES: protein motif:HMM:NF022779.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HIR1163299.1"
                     /translation="IYNEEMQVWENKKREVFKGQWLICDLGRELSAQVSLFPRTGFQR
                     LIRTDTIKTGNEVFDKHFTVRSDDGEKVFHILTPHMIEYILSAAEISGGKLYMSFLKE
                     GKLHIAVESDCDFFEAGKGSIDADRLREKFTTQIRWFTDMIDELRLTDTLYR"
BASE COUNT         1002 a         1019 c          686 g         1321 t
ORIGIN      
        1 atcgtataag gattataata aatattcaga cggtctgtcc ccagactcca ggcaacagca
       61 ccattgtcct gtggtgtatt gatacattca aaaaatacat ctgcatcttc ctcaggagta
      121 ctgttctcta ccagaccttc ttcgctggca tacttgtctt ttagttcgtg tacgatcccg
      181 gatttcacct tatcttcatc tgtgatcaca tcataaaaac ccagcttttt cccggttttt
      241 gtatcaaaag taagacctgt ataggaattg tatccatggg ctccgccgta atagtcataa
      301 taaaacagct cgtagcttac cacattttca tctgcgcgaa ggatatcggg tgtatacaca
      361 gctgacagct gcatattact atcctcctgt acatattcaa ccatctcctc tgccgatgtt
      421 ttcaggtttt ccagatcttc ttctgcactc ttttttaatg tgtcatactc caaagccagc
      481 gcctgattca gcttcggata agcagccgct tcctcatcgc ttaacacaag tccccctgtt
      541 ttcagactgt aacttacctg aacgctgcca tctgcactgt ctgcatatcc atttgcactg
      601 tctgtatgga tatagggatg taacatctgt ggcataccgt cattcgtttt ttttgactct
      661 gcattttctt ccgtctctgg tccgctttct gactcttctg caccactttc actttcattt
      721 acactttctt ttgttgctgc atccgcttcc tggctttcct ttgacgttgt ctctgctggt
      781 tctcccttta aaattttctg acatccagtt cctgtaaacg ccatgataac agcaagtcct
      841 gctgccataa ttcttttctg atttttggtg ttgcatagct ttattattta cttggtgtat
      901 tannnnnnnn nncatatctt ttctcccatt ttcaaattga ctcttgaagt tttggtaaaa
      961 atatagtatg atactagaga tgcgtttgta gctcagtgga tagagcagtg gcctccggag
     1021 ccgcgtgcgg aggttcgatt cctctcagac gcattttttt attacccttt ttcggtcttt
     1081 cccttgacaa atcctccccc atcccttatt ctgattacat atatttcaat atatcttcta
     1141 ttatctgtca atctgtaatc tcatcataat ttcattcatt ttatttttta taattcttat
     1201 ttctattatt ttactcctta ttttatatcc cggctacttt atttttacaa atgattttgc
     1261 tatggatctg cctgggcagt tttcataaat attattttta ggaggtatat cttttggtat
     1321 acgacacatt tctcaacaca gtaaaagaac agatggaaca ggctttagga gacggatatt
     1381 cccttacctt gcggaaagtc cagaaaaata atggtcttat cctggacggt ctgtgcattg
     1441 caaaaggtaa cggcaccatt gctccttcca tttatctgaa ctcctgttat gaacagtatt
     1501 taaacggaac ttcaatggag gagatcatcc agaagctgct cacagcttac tacgaaagca
     1561 gctggcctcc cattcttaac aagctggtcc tgtctgacta tgactctctc cgttcccaca
     1621 ttgctttccg tctgatcaac gcttcttcca atcaatccct tttaaaaaca atgcctcata
     1681 ttccctggat ggatcttgcc attgtctttt atctgtacat acaggaaaat gaaaacggca
     1741 tcatgaccgc agctatttct gatcaccata tgaaaacctg gggtgttaca gtccatgatc
     1801 tctaccacga ctctctttta aacactccca ggctttttcc tcctgttata tccagcatgg
     1861 cctgtatcct ggagtccctt gatcccaaac acctgcaaca tcagccagct ttcccggaaa
     1921 ctccttttta tgttctcacc aaccagaatg ggatcaatgg cgctgcctgc atcctttaca
     1981 aagatgttat aaaaaatttt gctgaaggga tggaccggga tatcctgatc cttccctcca
     2041 gcattcatga agttcttctg cttcctgatg atgacccgct atcttatgat gagctggggc
     2101 atctggtgac tcatatcaac cagacagaag tagttgccga agaccgtctt tccaatcagg
     2161 tctatctcta ttcccgtaaa gaagacagga tcaccactgt ttcttttggt ccttctgctg
     2221 tctgctgatt cttactgtac ccaggcaccg gagccatcta catatcgtcc gtcaggtgtc
     2281 caggtatttg catacataac accattggaa cccatatagt accatctgcc gcctactgcc
     2341 tgccagccgg tagtcatctc accactgcca ttcagataat accactggcc gcctaactgc
     2401 tgccagccag tcttcattgc acccgttgga tccagatagt accatttacc gcctactgcc
     2461 tgccagccgg tctgcatttc accgcttccg ttcatataat accactggcc tcctacaagc
     2521 tgccagccgg tagtcattac accatcacca tttaagtaat accactgtct gtctactctt
     2581 acccagccag tctgacgata accggatgta ttaaagtagt accatgctcc attgatctgc
     2641 ttccagccat tggatggata accgccgctt gaataggaat accaccagcc aacattatcc
     2701 tggacccatc tgccgattcc attgtaaacg cctgcctcat cttcatctac ataataggaa
     2761 gaagagtcat cagaccactc gcctgcctta tcattataac ttgcaatggc tcttacacgg
     2821 aatgtataat ctccttcctt attaaaatag ccattgaaat tgaaggtcgt tcctgtggtg
     2881 tttacagtgg tcactgtctt ctcatctctt aaaagtttta catcatagct ttttgcaccg
     2941 tcaatggagt cccattctgc aataccgtca ttccattcca ggttggaagt tccttccagt
     3001 tttccaccta tgcgttttaa ctgggctgtt acttcaatgt agtcgccgct gtcgtagatc
     3061 tttgctttct taaattctgc attacagccg gacagtttaa aatggctttt actggtatac
     3121 gagaagcgat aaccgtcctt tgcgtaaagt tccactttta cttccggtat gtcacctact
     3181 gtccaggtat cctggtcatc tgcattggta tactcagcgg actctacata atagctggat
     3241 gtatcaccgc ttacatggat cgaaccaata gagtctccgc tggcaggtgc cgtgtcatag
     3301 gaaaaagtca gtttcacagt gtcaatttta gtatctgctg caaatgcagg catcacggac
     3361 ataaaggtca ttgctgcagc taacgcgata caccaggctt ttttcatctt catattccat
     3421 cctccttctt atctattcat tagataaatt ataacgtttt taacttactt ttattataat
     3481 aaagagcatg gatctatgca atttcagaaa ccttaaaaag aacttacgat ttttgcaaaa
     3541 attttacatg gttacaaaat tatacactca ttttatctat aaagtgtatc tgtaagacgc
     3601 agttcatcga tcatgtctgt aaaccagcgg atctgtgtcg taaatttttc tctcagccgg
     3661 tctgcatcta tactgccttt gcctgcttca aaaaagtcgc agtcactttc caccgcaata
     3721 tgaagttttc cttcttttaa aaagctcatg tacagttttc cgccgcttat ctccgctgct
     3781 gaaaggatat attctatcat atggggtgtt aaaatgtgaa aaactttctc cccatcatca
     3841 gaacggaccg taaaatgttt atcaaaaacc tcattgcccg ttttaatggt atctgtacgg
     3901 ataagacgct ggaaaccagt ccttggaaac agactgacct gtgctgacag ctccctgcca
     3961 aggtcacaga tcagccactg ccccttaaaa acctcccgtt ttttattttc ccagacctgc
     4021 atttcttcat tatatata
//
LOCUS       DBMDUA010000225         4033 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8041_length_4033_cov_2.683113, whole genome shotgun sequence.
ACCESSION   DBMDUA010000225 DBMDUA010000000
VERSION     DBMDUA010000225.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8041_length_4033_cov_2.683113"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="ACZPCL_12380"
     CDS             <1..318
                     /locus_tag="ACZPCL_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR1163300.1"
                     /translation="ELDTILKSDPSLCYDYEYDRIPHFYTPFYVYQYATGFSAAIAIS
                     RKIFNGELGILEGYKKFLSGGCSDTPIELLKLAGVDMSHTGPVEEALKFFEELLNEFE
                     ELA"
     gene            499..1416
                     /gene="pyrF"
                     /locus_tag="ACZPCL_12385"
     CDS             499..1416
                     /gene="pyrF"
                     /locus_tag="ACZPCL_12385"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR1163301.1"
                     /translation="MISQLIEKIQKTKAPICVGLDPMLSYVPEHIVKKAFDAYGETLE
                     GAAEAIWQYNKEIIDNTYDLIPAVKPQIAMYEQFGIEGLMVYKKTVDYCQEKGLVVIG
                     DAKRGDIGSTSAAYATGHLGKVQVGSKSYSGFATDMLTVNPYLGTDGVKPFVDACNAN
                     DKGIFVLVKTSNPSSGEFQDKLIDGRPLYELVAEKVVEWGNMSMDGTYSNVGAVVGAT
                     YPEMSKILRKLMPHTYFLVPGYGAQGGTAADLKHCFNEDGLGAIVNSSRGIIAAYRQE
                     KYKQFGPEHFAEASRQAVIDMVADINSVL"
     gene            1449..2234
                     /locus_tag="ACZPCL_12390"
     CDS             1449..2234
                     /locus_tag="ACZPCL_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HIR1163302.1"
                     /translation="MAKLKMTAAVTSQVQLAEGIYDLRLAAGEIAAQAVPGQFVSLYS
                     KDGSRLLPRPISLCGIDKEKGELRLVYRVAGAGTKEFSALTAGDTIDVLGPLGNGFPL
                     LEGKKAFLIGGGIGIPPMLELAKALNKVNGGEMVQSVIGYRDSHMFLKEEFEAYGSVT
                     VATEDGSVGTKGNVLDAIRENDLKADVIYACGPTPMLRALKAYSQEKGILCYLSLEEK
                     MACGIGACLACTCQTKDVDEHSQVHNKRICKDGPVFLAEDIEL"
     gene            2302..3204
                     /locus_tag="ACZPCL_12395"
     CDS             2302..3204
                     /locus_tag="ACZPCL_12395"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR1163303.1"
                     /translation="MNMSVKIAGVEFKNPVMEASGTFGSGMEYSEFVDLSRLGAVVTK
                     GVANVPWPGNPTPRIAETYGGMINAIGLQNPGIDVFAKRDIPFLKKYDTKIVVNVCGK
                     TTEDYIDVVERLGDEPVDMLEINISCPNVKEGGIAFGQDPKAVEAITKAVKAHAKQPV
                     VMKLSPNVTDITVMAKAAEAGGADAISLINTLTGMKIDINRRTFAVANKTGGLSGPAI
                     RPVAVRMVYQAAQAVKVPIIGMGGICNADDALEFILAGATAVAIGTANFHDPYATVKT
                     VDGIKAYMEKYGIEDINELIGAVK"
     gene            3261..3941
                     /gene="pyrE"
                     /locus_tag="ACZPCL_12400"
     CDS             3261..3941
                     /gene="pyrE"
                     /locus_tag="ACZPCL_12400"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR1163304.1"
                     /translation="MESYKQEFIEFMVESNVLKFGDFTLKSGRKSPFFMNAGAYVTGT
                     QLKKLGEYYAKAIHDNFGLDFDVLFGPAYKGIPLSVATTMAISDLYGKDIRYCSNRKE
                     VKDHGDTGILLGSKIKDGDRVMIIEDVTTSGKSIEETFPIIKAQGDVEIKGLIVSLNR
                     MERGKGTKSALDEIKDLYGFPTAAIVTMADVTECLYNKECNGQVVIDDKIKAALDAYY
                     EQYGAKNE"
BASE COUNT         1262 a          805 c         1006 g          960 t
ORIGIN      
        1 gaattggaca ctatcctaaa aagtgatcct tccctctgtt acgattatga atacgaccgg
       61 atcccacatt tttatacccc attttatgta tatcagtatg ctaccggttt ttctgcagcc
      121 atcgctatca gcaggaagat ctttaacggg gaacttggca ttttagaagg atataagaag
      181 tttttaagcg gcggctgctc cgatacaccc atcgaactgt taaaactggc aggtgtggac
      241 atgtcccata caggcccggt agaagaggca cttaagttct ttgaggagct gttaaatgag
      301 tttgaggaac tggcataaaa ccagtttaga tccgctgcaa acatgacaat attgaaaaaa
      361 agtgtataat cccaagtttt cttgaagaaa taggttatct atgttaaaat taggataatc
      421 attcagaacg accagttttg gatcatggaa atacctgtaa tgaatataca aatgtagtgt
      481 aaaccaagga ggaacattat gatcagtcag ttaatcgaaa aaatccagaa gacaaaggct
      541 ccaatctgcg taggcttaga cccaatgctt tcctatgtgc cagagcacat tgtaaaaaaa
      601 gcatttgacg cttacggaga gaccttagag ggagctgcag aagcaatctg gcagtataat
      661 aaagaaatca ttgacaatac ttatgacctg atcccggcag taaagccaca gatcgctatg
      721 tacgagcagt ttggcattga gggattaatg gtttataaga aaaccgtaga ttactgccag
      781 gaaaagggtc tggtagtcat cggtgatgca aagagaggtg atatcggctc tacttccgct
      841 gcatatgcaa ccggacatct gggcaaagta caggtaggct ccaagtccta cagcggtttt
      901 gctactgata tgctgactgt aaatccatac cttggaacag atggcgtaaa gcctttcgta
      961 gatgcatgca atgcaaacga taagggaatt ttcgttctgg taaagacctc aaatccatcc
     1021 agcggcgagt tccaggacaa actgatcgac ggccgtcctc tttacgaatt agtagctgaa
     1081 aaagtagtag agtggggcaa tatgtccatg gacggaacct acagcaacgt aggtgcagta
     1141 gtaggcgcta cctatccgga aatgagcaag atcttaagaa agctgatgcc acatacctac
     1201 ttcctggtac caggatacgg cgcacagggc ggtactgcag cagacttaaa gcactgcttc
     1261 aatgaagatg gtttaggtgc tattgtaaac tcctccagag gtatcattgc agcatatcgt
     1321 caggaaaaat acaaacagtt tggtccggag cactttgcag aggcttccag acaggcagtt
     1381 attgacatgg ttgctgatat caacagtgta ctgtaattag caagaaataa aaaggttgga
     1441 aaaacaacat ggcaaaatta aaaatgacag ctgctgttac ctcacaggta cagctggcag
     1501 aaggaattta tgacttaaga ctggctgcgg gtgagatcgc agcccaggct gtgcctggac
     1561 agttcgtatc cctttattcc aaagacggat ccagactgct tccaagaccc atcagccttt
     1621 gtggtattga taaagaaaag ggagaactgc gtctggtata ccgtgtggca ggagccggaa
     1681 caaaggaatt ttccgcactg acagcaggag atactattga cgtattagga cctcttggaa
     1741 atggtttccc attattagaa ggaaagaaag cattcctcat cggcggcggt atcggtatcc
     1801 cgccaatgct tgaacttgca aaagcattaa acaaagtaaa cggtggtgaa atggtacagt
     1861 ccgttatcgg ctaccgtgac agccacatgt tcttaaaaga agaatttgaa gcatacggct
     1921 ctgtaactgt tgccacagaa gacggcagtg taggaacaaa gggaaatgta ttagatgcca
     1981 tccgtgaaaa tgatctgaag gcagatgtga tctatgcctg cggaccgact cctatgcttc
     2041 gtgccttaaa ggcatacagc caggaaaaag gcatcctctg ctatttatcc ctggaagaga
     2101 aaatggcctg cggcattggt gcctgtctgg catgcacctg ccagacaaag gatgtggacg
     2161 aacattccca ggtacataac aagcgtatct gtaaggatgg ccccgtattc ctggcagaag
     2221 acattgaact gtaaagtaca gtaaaaattg cccggatcag aacgggcgga catgtgagaa
     2281 agcaggaggc aggaccgaat tatgaatatg agtgtaaaga tcgccggagt ggaatttaaa
     2341 aatccggtta tggaagcgtc cggcaccttt ggttccggta tggaatacag cgagtttgta
     2401 gatttaagcc gtcttggcgc tgttgtcaca aaaggtgtgg caaatgtgcc gtggccggga
     2461 aacccgacac caaggatcgc agagacctac ggcggcatga tcaatgccat tggtctgcag
     2521 aacccgggta tcgatgtatt tgcaaagaga gatatcccat ttttaaagaa atatgataca
     2581 aagatcgtag ttaatgtgtg cggaaagacc acagaagatt acattgatgt agtagagcgc
     2641 ctgggagatg agccggtaga tatgctggag atcaacattt cctgtccaaa tgtaaaagag
     2701 ggcggtatcg ccttcggtca ggatcctaag gcagtagaag ctattacaaa ggcagtaaaa
     2761 gcccatgcaa aacagccggt tgttatgaaa ctaagcccga atgtaacaga tattacagtt
     2821 atggcaaagg cagcagaggc aggcggcgca gatgccattt ccctgatcaa tactttaacc
     2881 ggtatgaaga tcgacatcaa ccgcagaacc ttcgcagttg ccaataaaac aggcggactt
     2941 tccggtccag ctatccgtcc ggtagctgta cgcatggtct atcaggcagc ccaggcagta
     3001 aaagtgccga tcattggtat gggcggtatc tgcaatgcag atgacgcgct ggaatttatc
     3061 cttgcaggcg caactgcagt agctatcggt actgcaaact tccacgaccc atatgcaact
     3121 gttaaaacag tcgatggcat taaggcatat atggaaaaat atggaattga agatattaac
     3181 gaactgatcg gtgcagtgaa gtaataaaac agtcacataa gaaatttgga aaaaattgga
     3241 aaaataagga gggcattacc atggaaagct ataagcagga atttattgaa tttatggtag
     3301 aaagcaatgt attaaaattt ggagatttca ccttaaagag cggaagaaag tccccattct
     3361 ttatgaacgc aggcgcctat gtaacaggta cccagttaaa gaaattaggt gagtactacg
     3421 caaaggctat tcacgacaac tttggtctgg actttgacgt actctttgga ccggcttaca
     3481 agggtattcc gttaagcgtt gcaaccacca tggcgatcag cgatttatac ggaaaagaca
     3541 tccgttactg ctccaaccgt aaggaagtaa aggaccacgg tgataccggt atcctgttag
     3601 gaagcaagat caaagacggc gaccgtgtta tgatcattga ggacgttacc acttccggta
     3661 agtccattga agagacattc ccaatcatta aggctcaggg cgatgtagag attaagggtc
     3721 tcatcgtttc cttaaaccgt atggagcgtg gaaaaggaac caagagtgct ttagacgaga
     3781 ttaaagatct gtacggcttc ccaactgcag ccatcgttac catggcagat gtaacagagt
     3841 gcttatacaa caaggaatgc aacggtcagg tagtgatcga tgataagatc aaggcagctc
     3901 ttgatgcata ttatgaacag tacggcgcaa agaacgaata agagccggag gtagttgcaa
     3961 tatggagaga gtatataaaa caatgaaggt tacaggagcc ggctgtattg cagcaggtgt
     4021 tgtgatcctg gtg
//
LOCUS       DBMDUA010000226         4003 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8106_length_4003_cov_3.077652, whole genome shotgun sequence.
ACCESSION   DBMDUA010000226 DBMDUA010000000
VERSION     DBMDUA010000226.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8106_length_4003_cov_3.077652"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            135..1124
                     /locus_tag="ACZPCL_12405"
     CDS             135..1124
                     /locus_tag="ACZPCL_12405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR1163305.1"
                     /translation="MCVYNDTGKNTTSASETLLNKRIAIVGIGGVGGYLGAMLAHAYP
                     HVTLAARGKRLEHIKEHGLTLHSVYSGEITAFPEHVTTADAIGPQDYIFVCVKNYSLE
                     DVCRQITPFVDDHTVLIPVMNGVDTGERMRNLLKKGTVIDSLIYIVAYASPDFSITQI
                     GNLASLRIGIKNASKEQSENVLEASALLSGAHIHHKIADNIIAAIWRKYILNCAYNIT
                     TARYNRTIGQLREDPETAAQYETLVRESWLVSEAAGAGVPETSINEIIDQFYHKLDPA
                     STSSLQRDIWAGRPSELETFSGYLVRKAKELSVPVLLSETMYSDLQKIVSKQS"
     gene            complement(1157..1735)
                     /locus_tag="ACZPCL_12410"
     CDS             complement(1157..1735)
                     /locus_tag="ACZPCL_12410"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1163306.1"
                     /translation="MGKVENEGMPVVRLDRQLKYQGNILKIYEDTVLANGHEAHWDYI
                     HHDGAAAVLPVTSEGKILMVRQYRNALNRYTLEIPAGKLDAPDEPKIECAYRELEEET
                     GFRTEKLEYLMSINTTVAFCDEAIDIFVAHNLIPSHQHLDEDEVIEVEAWELKDLEEM
                     IYTGKITDGKTIAALMAYGRKYRSVDKKDELA"
     gene            complement(1859..2653)
                     /gene="proC"
                     /locus_tag="ACZPCL_12415"
     CDS             complement(1859..2653)
                     /gene="proC"
                     /locus_tag="ACZPCL_12415"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR1163307.1"
                     /translation="MAKIGFIGMGNMGSAILNGLLRVYKPEDLLFTAAHKEKMEAVTA
                     KTGVVHAASNAECAKQSEYVILAVKPQYFETVFEEIRPVLKEDQIVISLAPGQTIASL
                     TERLGGKVRVVRTMPNTPALLGEGMTGMAYEESNYSEEEKAVLRGIFEACGRLEVVDE
                     RMMDAVGCVSGCSPAYVYMFIEALADGAVKYGLPRAKAYQMAAQTVLGSAKMVLETGK
                     HPGQLKDEVCSPGGTTIAGVSALEEWGFRNAVIKAEDASYEKGRKM"
     gene            complement(2765..3994)
                     /locus_tag="ACZPCL_12420"
     CDS             complement(2765..3994)
                     /locus_tag="ACZPCL_12420"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR1163308.1"
                     /translation="MAKTYSTPFYLYDEKGIRENVKHLKQAFSWNKGYKEYFAVKATP
                     NPFILKILQEYGCGTDCSSLTELMMSKACGFSGSNVMFSSNDTPPEEFAYANEMGAII
                     NLDDITHIQCLEDTLGFIPETISCRFNPGGVFKISNDIMDNPGDAKYGMTTEQIFEAF
                     KILKSKGAKNFGIHAFLASNTVTNEYYPMLAKILFELAVKLKEETGAHISFINLSGGI
                     GIPYRPDQEPNDIMAIGEGVRKVYEEVLVPAGMDDVALCTELGRFMLGPYGCLVTKAI
                     HEKHTHKEYIGVDACAVNLMRPAMYGAYHHITVLGKEDAPCDHKYDITGSLCENNDKF
                     AIDRMLPEIKMGDFLVIHDAGAHGFAMGYNYNGKLKSAELLLKEDGSVQMIRRAETPK
                     DYFATFDFCGIMDGYDK"
BASE COUNT         1052 a         1005 c          803 g         1143 t
ORIGIN      
        1 aaaaatgagc gaattacgaa tgtttttaga attgcgagag tgcaaagcac gtagcaattc
       61 ggtatcagag ggggcaaaat accttttgac cccaacatca tgatatatat ttaataacag
      121 gaggttttct gcctatgtgt gtatacaatg ataccggtaa aaatacaacc tctgcctctg
      181 aaactctttt gaataaaagg atcgctatag taggcatcgg cggcgtaggc ggctatttag
      241 gtgccatgct ggctcatgcc tatccccatg ttacactggc agccagagga aaacggctgg
      301 aacatataaa agaacatgga cttactcttc acagtgttta cagcggagag atcacagcct
      361 ttcctgaaca tgtaacaaca gccgatgcca ttggtcccca ggattatatt tttgtatgcg
      421 taaaaaacta ttccttagag gatgtctgcc gccagatcac gccttttgtg gatgatcaca
      481 ccgttctcat accggtaatg aatggcgtag atacaggaga gcggatgaga aatcttctga
      541 aaaaaggcac tgttatcgac tcccttattt atatcgttgc ctacgcatcc ccggactttt
      601 ccattaccca gatcggcaac ctggcttccc tgcggatcgg cataaaaaat gcctcaaagg
      661 agcagtcaga gaacgtcctt gaggcatccg cccttttaag cggggcacat atccatcata
      721 aaattgccga taacattatc gcagccatct ggcgcaaata cattttaaac tgcgcctata
      781 atatcactac cgcccggtat aaccggacca ttggacagct tcgggaagat cctgagacag
      841 ccgcccagta tgagacctta gtccgtgagt cctggcttgt atctgaggca gccggtgcag
      901 gcgtgccgga aacttccatt aacgagatca tcgatcagtt ctaccataaa ctggatccgg
      961 catccaccag ttcccttcaa agagatatct gggccggacg ccccagtgag ctggagacct
     1021 tcagcggcta tctggtacga aaagcaaagg aactttccgt accggtgcta ttgtcagaaa
     1081 ctatgtattc tgacctgcag aaaattgttt caaaacaaag ctgacatacg cctgacaacg
     1141 gcattatcac agatatttaa gccagttcat ctttcttgtc tacagagcgg tacttgcggc
     1201 cataagccat cagtgccgct attgttttgc cgtcagtgat ctttcctgtg tagatcatct
     1261 cttccagatc ttttaattcc catgcttcca cttcgatcac ttcatcttca tccagatgct
     1321 gatgggacgg gatcaggttg tgggctacga aaatatcaat ggcttcatca caaaatgcaa
     1381 ctgtagtatt tatactcatg agatattcca gtttttcggt gcggaaacca gtttcctcct
     1441 ccagttctct gtaagcacac tcgatctttg gctcatccgg tgcgtccagc tttcctgccg
     1501 ggatctccag tgtatagcgg tttaaagcat tgcggtactg gcgcaccatt aagatctttc
     1561 cttctgaagt tacaggcaga acagccgctg caccatcgtg gtggatgtaa tcccagtgtg
     1621 cctcatggcc atttgcaaga actgtatcct catatatttt caggatattt ccctgatatt
     1681 ttaactgacg gtctaaacgt actaccggca ttccttcatt ttctactttt cccatgtttc
     1741 ttctcctttt atatgttatg tactcccgaa ctcttcttgc aaaatcagac ataaaaaatt
     1801 ccaggagcat tgatctttca cacctccgcc tgcagtgcag gcgatgtgcc acatacattt
     1861 acattttcct gcccttttca tagctggcat cttctgcctt gatcacagca tttctgaagc
     1921 cccactcttc cagtgcagat acaccggcaa tggtggttcc gccaggtgag caaacctcat
     1981 cctttaactg tcccggatgc tttcctgttt ccagtaccat tttagcactt ccaagaacgg
     2041 tctgggctgc catctggtat gccttggccc ttggaaggcc gtattttaca gctccgtctg
     2101 ccagcgcttc aataaacatg tatacataag ccggtgaaca gccgctgaca cagccaactg
     2161 catccatcat acgttcatct actacttcca gacgtccaca ggcctcaaaa ataccacgaa
     2221 gcactgcctt ttcctcttca ctgtagttgg actcctcata tgccataccg gtcatgcctt
     2281 cacccagtaa cgccggtgta tttggcatgg tacggactac gcgcaccttt ccgcccagac
     2341 gttctgtaag gcttgcaatg gtctggccag gtgccagtga gatcacgatc tgatcttcct
     2401 ttaataccgg acggatctcc tcaaacactg tctcaaaata ctgtggcttt actgccagga
     2461 tcacatactc agactgcttt gcacactctg cattggatgc tgcatgaacc actcctgtct
     2521 ttgctgttac agcttccatc ttttctttgt gggctgctgt aaataataag tcctccggct
     2581 tatagacccg taaaagtccg tttaaaatag cggaacccat atttcccatt ccaataaaac
     2641 cgatctttgc catatttttc gcctcctgtt ataattttta aaacattcac gaatattcgt
     2701 ccattttaac gcacacaccc gcccgcatac gcggacgggc aaacgttctt tctatatgtg
     2761 atttttattt atcataccca tccatgatac cacaaaaatc aaaggttgca aagtaatcct
     2821 ttggagtttc tgcgcggcgg atcatctgga cagagccgtc ttcctttaat aaaagttctg
     2881 cggattttaa cttaccgttg tagttataac ccattgcaaa gccatgggca cctgcatcat
     2941 ggatcactaa aaagtcaccc attttgatct caggaagcat acggtcaatc gcaaatttat
     3001 cattgttctc gcaaagggaa ccggtaatat cgtacttgtg gtcacaaggc gcatcttctt
     3061 ttccaagaac ggtaatgtga tgataagcac catacatagc cggacgcatc aggtttactg
     3121 cgcaggcatc tacaccgatg tactctttat gagtatgttt ttcatggatc gctttggtaa
     3181 ctaggcatcc ataaggccct aacataaaac ggccaagctc tgtgcaaaga gccacgtcat
     3241 ccattcctgc aggaaccaga acttcctcat aaactttgcg cacgccttcg ccaatagcca
     3301 taatgtcatt tggttcctga tctggacgat aagggatacc aataccaccg gacaggttga
     3361 tgaaactgat atgtgcacct gtctcttctt ttaacttaac agccagctca aagaggatct
     3421 ttgcaagcat cggataatat tcattggtaa ctgtattgct tgccaggaaa gcatggatac
     3481 cgaaattctt tgccccctta gactttaaaa tcttaaatgc ctcaaagatc tgctcagtag
     3541 tcataccata ctttgcatct cccgggttgt ccataatgtc attgctgatc ttaaacacac
     3601 cgcctggatt gaaacggcag ctgatggtct ccggaatgaa gcctaatgta tcttccaggc
     3661 actggatatg ggtaatatca tccaggttga tgatggcacc catttcattt gcgtaagcaa
     3721 attcctcagg cggagtatca ttggaagaaa acattacatt ggaaccggaa aaaccgcagg
     3781 cctttgacat cattaactct gttaaagaag agcagtctgt accacagcca tattcctgta
     3841 agatctttaa gataaatgga tttggggttg cttttactgc aaaatattct ttatatcctt
     3901 tattccagga aaatgcctgt ttcagatgtt ttacattttc gcggatacct ttttcatcat
     3961 acagataaaa tggggttgaa taggtcttgg cgatctcttc tgc
//
LOCUS       DBMDUA010000227         3963 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8199_length_3963_cov_2.724236, whole genome shotgun sequence.
ACCESSION   DBMDUA010000227 DBMDUA010000000
VERSION     DBMDUA010000227.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8199_length_3963_cov_2.724236"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="ACZPCL_12425"
     CDS             <1..534
                     /locus_tag="ACZPCL_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT protein"
                     /protein_id="HIR1163309.1"
                     /translation="LPEDIKLRMGNIELLFRFLSSLKILRAKDIGPIVKSVKFEKDKR
                     TISQQIIDQIISGNEKRYQAYQKLRSDDSNLTVEHQEAINYFFGQWVPLENLLNRVTK
                     RSSRSVSVFNINALKRLGVLDKECINQIAFLRKMRNSLIHDIEVPEASYIREQGEEAK
                     VLLEKLTKLLETKTAEG"
     gene            complement(634..1635)
                     /locus_tag="ACZPCL_12430"
     CDS             complement(634..1635)
                     /locus_tag="ACZPCL_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3878 family protein"
                     /protein_id="HIR1163310.1"
                     /translation="MRLDCSGMEFEKLADLLEQGQFELLNLPVGDERKKPSDIRLVYM
                     MNDTAESFLVFHEAVLTGNYLPDYEGELSASLDKEETDNTDAEGEEKGRYILAVHQGD
                     TVCTLFFKNISLECELYDYGGIGHFWVKGYEYLRQLEYKIAILGDKWEYLGEDCCSEE
                     EKKLAVLREFPPLSYLFYPAASLAYVREKEEPWKCSPLAAKLVEEAAKQEKDTWFAKM
                     VSLYKRYPVRVLARYIAWMFHREAHKRVTDHLIRQMDQAAAIYPRRYFGEENEKKHKI
                     LFEKANKRKRDLEKQGIQAEIFREEPFAKGDDGPFKVYVMQFTVKKGNHISAVEEIR"
     gene            complement(1632..2657)
                     /locus_tag="ACZPCL_12435"
     CDS             complement(1632..2657)
                     /locus_tag="ACZPCL_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR1163311.1"
                     /translation="MEPYYYTKMSKPQQAVYYAIYQGLMALSDSFQVPKLEGRELSDV
                     FFQLRLDHPEIFWAEGFHYRYYQDSANITFLPEYIFEKGKIKEHQKALKARVEKLVRP
                     AMKLSEWEKEKYVHDFICENVHYDKLKKSYSHEIIGPLGQGVGVCEGIAKSVKVLCDA
                     LGIWCMIAICGNNPEKGIKYRHTWNIVKIGGTYYHLDATFDNTLGKHSFGAKEIRYDY
                     FNLDDKNIFRDHEPLIAPAPACTDGDHFYYKEKKMSFTKTEDVYKRSLQAAKKGKMLT
                     FHWRGGYLTRAVLDELLDLIKKAGAEKGKVARIALNWPQAVLRLEYVEDGQENVTMEE
                     ANEGEQE"
     gene            complement(2907..3857)
                     /locus_tag="ACZPCL_12440"
     CDS             complement(2907..3857)
                     /locus_tag="ACZPCL_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008373299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1163312.1"
                     /translation="MIKIDLITGFLGAGKTTFIKKYASYLMKQGKKIGILENDFGAIN
                     VDMLLLQDLEGDNCTLEMIAGGCDADCHRRRFKTKLIAMAMEGYDRVIVEPSGIYDVD
                     EFFDTLYEEPLDRFYEPGNVIALVDAGLEEDLSKEAKYLLASEVADAGCVLFSKCDEV
                     SQEQLKATLSYLNQAMEQVHCKRRFTLDQILCKNWEDLEEADYKRLLNCEYRMEDYEK
                     MHLEQGKVFDSLYYLNFSMDPDQLKTAVENMFRDPSCGKIFRIKGYIKKGENGWLELN
                     ATSREFSLKPAPKGQEVLIVIGENLSKEAAALYLGRKPDV"
BASE COUNT         1043 a          975 c          708 g         1237 t
ORIGIN      
        1 cttccggaag atatcaagct tcgtatggga aatattgagc tgctgttccg ctttttaagc
       61 agcttaaaga tattacgggc aaaagatatc ggacctatcg taaagtctgt aaaatttgaa
      121 aaagacaaac gtaccatttc ccagcagatc attgaccaga tcatcagcgg aaatgaaaag
      181 agataccagg cataccagaa gctgcgctcc gatgactcca atttaacggt ggaacatcag
      241 gaagccatca actacttctt tggccaatgg gtgcctcttg aaaatctctt aaaccgtgtg
      301 accaagcgca gttcccgttc cgtaagcgta tttaatatca acgctttaaa acgactggga
      361 gtgcttgata aagaatgtat taaccagatc gcctttttac gaaaaatgcg aaattccctg
      421 atccatgata ttgaagtacc ggaagcttct tatatccggg aacagggcga ggaagcgaaa
      481 gttttactgg aaaaattaac gaaattatta gaaacgaaaa cagcagaggg gtaaattcct
      541 ctgctgtttt tatataatag atattgtctg caaaacctgc caacagccgc acggggctga
      601 tgcatagcag tatgtggtta tgatcatttg caattatcgg atttcttcaa ctgcagatat
      661 gtgatttccc ttcttaactg taaactgcat tacatacact ttaaagggtc catcgtcgcc
      721 ttttgcaaaa ggttcttctc taaagatttc cgcctgtatc ccctgttttt caagatctct
      781 tttacgtttg ttggcttttt caaacagaat tttatgcttt ttttcgttct cttctccaaa
      841 ataacgtctt ggataaatgg ccgctgcctg atccatctgc cggatcagat gatcggttac
      901 tctcttatgg gcttctctgt gaaacatcca ggcaatgtat ctggcaagta ccctgacggg
      961 gtatctttta tacagggata ccatttttgc aaaccaggta tctttctcct gttttgcagc
     1021 ctcttccacc agttttgctg ccagcggcga acatttccac ggttcttctt tttctcttac
     1081 ataggccagt gaagctgccg ggtaaaacag atagcttaac ggcggaaatt ctcttaaaac
     1141 tgccagcttt ttctcttctt cactgcagca atcttctccc agatattccc atttatcccc
     1201 taaaatagcg atcttatatt ctaactggcg cagatactca tatcccttca cccagaaatg
     1261 accgatcccg ccgtaatcat acagctcaca ttccaggctg atatttttaa aaaacagggt
     1321 gcagactgta tctccctgat ggaccgccag gatataccgc cccttttctt caccttccgc
     1381 gtccgtgtta tctgtttctt ctttatccag ggaagcgctt agttctccct cgtaatccgg
     1441 caggtaattt cctgtaagca ctgcctcatg gaataccaga aagctttccg ctgtgtcatt
     1501 catcatatac accagacgga tatccgatgg ctttttcctc tcatcaccaa ccggcagatt
     1561 caaaagttca aactgcccct gttccaacag atctgccagc ttttcaaatt ccattcccga
     1621 acagtctaat ctcactcctg ctctccttca tttgcctctt ccatagtaac attttcctgg
     1681 ccatcttcca catactccag acgcagcact gcctgaggcc agtttaaagc aatacgggct
     1741 acttttccct tttctgctcc tgcttttttg atcagatcca gcaattcgtc caggactgcc
     1801 cttgtaagat aaccgcctct ccagtgaaag gtaagcattt ttcccttttt cgccgcctga
     1861 agagaacgtt tatacacatc ttctgtcttt gtaaaggaca ttttcttctc tttgtaataa
     1921 aaatgatccc cgtctgtaca ggccggcgcc ggcgcgatca gcggctcatg gtcccggaaa
     1981 atatttttat catccagatt aaagtaatcg tagcggatct cctttgcacc aaagctgtgc
     2041 ttacctaacg tattgtcaaa ggtagcatcc aggtgataat acgtgccgcc aatttttaca
     2101 atattccagg tatgacggta tttgatccct ttttcaggat tatttccgca gatagcgatc
     2161 atacaccaga tacctaaggc gtcacaaagc acttttacag atttggcgat cccttcacat
     2221 acccctacac cctgtcctaa aggaccgatg atctcatgag aataagattt ctttaactta
     2281 tcataatgta cattttcaca gataaaatca tgtacatact tttctttttc ccattccgaa
     2341 agcttcatag cagggcggac cagcttttcc actctggctt ttaatgcttt ctggtgttct
     2401 ttgatctttc ctttttcaaa aatatactcc ggcaaaaaag tgatattggc cgagtcctga
     2461 taataccggt aatgaaatcc ctctgcccag aagatctccg gatggtccaa acgcagctgg
     2521 aaaaatacgt cgcttagttc ccgtccttct aatttcggca cctggaagct gtcggaaagt
     2581 gccatgagcc cctggtaaat ggcataatag acagcctgtt gtggcttgct catctttgta
     2641 tagtaatatg gttccataat aatttccttt catgatcaga tgaaagtcac cgtaaaaaag
     2701 ctgatgtcat ctgcaatcca tctgtctcac agaagccatc agctttttct taatgtcctc
     2761 tcaaaatctt tctgtgcctg attttgtgag aacattttat ttatgcacag agcatctgca
     2821 actctgccat atctgtcctt gtccaacggg aaatattata acctgtccct tatcttttgt
     2881 aaactgtttt ttgtttaaaa cctggcttaa acatccggtt tacggcctaa ataaagagct
     2941 gctgcctctt ttgaaaggtt ttcgccaata acgatcagca cttcctggcc ttttggcgcc
     3001 ggtttcagag aaaattccct gctggtggca tttaactcca accagccgtt ttctcccttt
     3061 tttatatagc ctttgatgcg aaagatcttt ccgcaggaag gatccctaaa catattttct
     3121 actgctgttt ttagctgatc cggatccatg gaaaaattca gatagtacag agaatcaaac
     3181 actttcccct gttctaaatg cattttttca taatcttcca tgcgatattc acagttgagc
     3241 agacgcttat aatcagcctc ttccagatct tcccagtttt tgcacaggat ctgatccagg
     3301 gtaaaccttc gtttacaatg tacctgttcc attgcctggt tcagataaga aagggtggct
     3361 ttcaactgtt cttgagacac ttcatcgcat ttactgaaaa gcacgcagcc tgcatctgct
     3421 acttctgatg ccagcagata ttttgcctct tttgaaagat cctcttccag gcctgcatcc
     3481 accagtgcga tcacatttcc cggctcataa aagcggtcta acggctcttc ataaagggtg
     3541 tcaaaaaatt catccacatc ataaatgcca gacggctcta cgatcacccg gtcatagcct
     3601 tccatagcca ttgcgatcag cttggtttta aatctgcgtc tgtggcagtc tgcgtcacag
     3661 cctccggcga tcatttccaa tgtgcagtta tccccttcta aatcctgtaa aaggagcata
     3721 tccacattga tggcgccaaa atcattttcc agtattccga tcttctttcc ctgtttcatc
     3781 agataagaag catacttttt tataaaagtt gtcttcccgg cacctaaaaa tccggtgatc
     3841 agatcgatct ttatcatatc tttcactcct cgtataatat cttttagata cataaataca
     3901 tatattttgg ttaataagtt actaaataaa tatttgttca ataggcttag taaagcccat
     3961 gat
//
LOCUS       DBMDUA010000228         3957 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8215_length_3957_cov_2.146266, whole genome shotgun sequence.
ACCESSION   DBMDUA010000228 DBMDUA010000000
VERSION     DBMDUA010000228.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8215_length_3957_cov_2.146266"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(261..1547)
                     /gene="tig"
                     /locus_tag="ACZPCL_12445"
     CDS             complement(261..1547)
                     /gene="tig"
                     /locus_tag="ACZPCL_12445"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR1163313.1"
                     /translation="MSVQVEKLEKNMAKLTVEVSAEDFKAAIKKAFNKNKNRFAIPGF
                     RKGKAPQAMIEKMYGEGVFYEDAADEAINASYAEAMKESGLDIVSRPEVTIEKIGKDE
                     PFVYSALVAVKPEVTLGQYKGVEVEKADASVSAEDVEAELKKVQEQNARLLTVEDRGV
                     EDGDQTVIDFEGFVDGKGFEGGKAEDYPLTIGSHSFIDTFEEQLIGKKIGEECEVNVT
                     FPTEYHAADLAGKPATFKVTVKEIKVKELPELNDEFASEVSEFDTLDEYKKDVEKKLA
                     EKKEIEANSKNEDAVVAKVVENATMEIPDKMIDAQAENMVQDMARRMQSQGLSLDMYL
                     KYTGMTVEQMKEQARPDAEKRIRTRLVLEAVAKAENIQISDEKVDEEVAKMAEAYKME
                     VEKLKSYMSESDVKQMKEDLAVQQAVDLLVAEAKLA"
     gene            1808..2875
                     /locus_tag="ACZPCL_12450"
     CDS             1808..2875
                     /locus_tag="ACZPCL_12450"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1163314.1"
                     /translation="MSVTLKDVAEKLGVTITTVHKALNGKEGVSEKRRAEIMKVAAQM
                     GYKVNYMASSLSKKKFHIAVVLPNTDGDNRFYYGALWDGIRNYLDTVSEFPITVSEYA
                     YPLTRDGNGLVLEKIFNQDSDDLQGLITIAMDHDRSSYFLEKFAKKQIPVVLIGADLH
                     TDFRLCCIKANDTAVGYLAAELLTAFLPSGYPAKILMAGDYGCLGMEDQRLNMQAFSA
                     YLHTYAPSIETVPFQGSDPLAVSKEIRQYLTVHPDTYAIYTCSARFTYHISQCIRQLG
                     IQDRIQVIGNDLFTESRQALSDGILRGVIDKKISKQSALAVKTLFDYLLKKDYPRSSC
                     LVMEPEIVLRSNFQSHSDLQK"
     gene            complement(2970..3605)
                     /locus_tag="ACZPCL_12455"
     CDS             complement(2970..3605)
                     /locus_tag="ACZPCL_12455"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HIR1163315.1"
                     /translation="MRIALINENSQAGKNEIIYRELKGVVEPMGHVVDNYGMYSADDE
                     AQLTYVQCGILASLLLSSGAADFVVTGCGTGQGAMIACNSFPNVVCGHVSNPSDAFLF
                     AQVNDGNCVAMPYAQNYGWGGELNLRYSFEKLFGFGSGNGYPPERVVPEQRNKKILDQ
                     VRANNFKDVITCCKGLDQELVKGAVAGPKFKELFFANCKDEAIKAYVETLL"
     gene            complement(3694..>3957)
                     /locus_tag="ACZPCL_12460"
     CDS             complement(3694..>3957)
                     /locus_tag="ACZPCL_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR1163316.1"
                     /translation="RVLAYCDNVMCVENTFKKGSIGAMHNHPHTQITYVASGKFEFTI
                     DGEVHTVSKGDTLLKQDGVMHGCTCLEDGILIDIFTPMREDFL"
BASE COUNT         1010 a         1027 c          741 g         1179 t
ORIGIN      
        1 tctgcgcttc aatggttacg atttccatac gtttctcttt tttatccggt acgaaaatag
       61 ggatggaatc cctttcttcc aaaaaggtgt ctgctcaatg acataaggta ctaaactcat
      121 ataaccatcc tccgttatga tctttatatc tgttttccac cccatagcgg gaatttttcc
      181 cagatatacg tttaatatac aaaaccaagg agactctcag cagagaatcc ccccggtctc
      241 ttgtgtacaa cttttaataa ttaagccagc ttagcttctg ctactaacag atcaactgcc
      301 tgctgtacag ctaagtcttc cttcatctgc ttaacatcgc tttcgctcat gtagctcttt
      361 aacttctcaa cttccatctt gtaagcttct gccatcttag ctacttcttc gtcaaccttc
      421 tcgtcagaga tctggatgtt ctcagcctta gcaactgctt ccagaaccag tctggtgcga
      481 attctcttct cagcatccgg acgagcctgt tccttcatct gctctacagt catgccggtg
      541 tactttaagt acatatccag agacagtccc tggctctgca tacggcgagc catatcctga
      601 accatgttct cagcctgagc gtcgatcatc ttgtccggga tctccatggt tgcgttctca
      661 actaccttag caactacagc gtcttcgttc ttggagtttg cttcgatctc tttcttctca
      721 gctaacttct tctcaacgtc cttcttgtac tcgtctaaag tatcgaattc agaaacttca
      781 gatgcgaact catcatttaa ctctggaagt tcttttacct tgatttcctt aacagttacc
      841 ttgaaggtag ctggctttcc agccagatct gctgcatggt actcggttgg gaaggttacg
      901 tttacttcgc actcttcacc gatcttcttg ccgatcagct gctcctcgaa ggtatcgata
      961 aaggaatggg aaccgatggt taatggatag tcctcagcct ttccgccttc aaagcccttg
     1021 ccgtctacaa atccctcgaa gtcgatcacg gtctggtcac cgtcttctac tccacggtct
     1081 tcaacagtaa gcagtcttgc gttctgctcc tgaaccttct ttaattcagc ctcaacgtcc
     1141 tctgcactta cagaagcgtc agccttctct acttctacgc ccttgtactg gcctaaagta
     1201 acttctggct taactgctac taatgcgctg tatacgaatg gctcatcttt gccgatcttc
     1261 tcgatggtaa cttctggtct ggaaacgata tccagtccgc tttccttcat agcctcagcg
     1321 taggaagcgt tgattgcttc atcagctgca tcctcataga atacgccttc gccatacatc
     1381 ttttcgatca tagcctgagg agcttttcct ttacggaaac ctgggatcgc aaagcggttc
     1441 ttgttcttgt tgaacgcttt cttgatcgca gccttaaaat cctcagcgct gacttctaca
     1501 gtcagttttg ccatgttttt ctctaatttt tctacctgta cactcatttc tacgggttcc
     1561 tccttgaaaa tatatgtgat tcgcccatat ccatgagggc gctacacttc aagttagcat
     1621 tataacatgg cagtcagaaa aacgctagta ttttttgata actttcataa aaaagcacac
     1681 aatctgctgc aatttccggc agaacagtct caccaaataa agttctatcc ccagaaaact
     1741 attgtttaat cttcttaaag tcgatatact tatttcagaa aaactcacag agaggcggat
     1801 atagactatg tcagtgacat taaaagatgt tgcagaaaaa ttgggagtaa caatcaccac
     1861 agttcacaaa gcgcttaacg gtaaagaagg cgtcagcgaa aaacgacgcg cagaaatcat
     1921 gaaagttgct gcgcagatgg gatacaaagt taactatatg gcatcctctc tctcaaagaa
     1981 aaaatttcac attgcagttg ttctgccaaa tacagatggg gataaccgtt tttactatgg
     2041 tgctctctgg gacggcatcc gaaattatct ggataccgta agtgaatttc ctatcacagt
     2101 ttcagaatat gcctatccgc ttacccgtga cggcaacgga ctggtcctgg aaaaaatatt
     2161 caaccaggac tctgatgatc tccagggatt gatcaccatt gccatggacc atgaccgctc
     2221 ttcttatttc ctggaaaaat ttgccaaaaa gcagatcccg gtggtgctta ttggcgctga
     2281 tcttcataca gattttcgtc tttgctgcat caaagcaaat gatacagctg tgggctatct
     2341 tgctgcagag cttctcactg cttttcttcc atccggctat ccggcaaaga tccttatggc
     2401 cggagactat ggctgccttg gcatggagga tcagcgcctt aatatgcagg cattttcagc
     2461 ttacctgcat acctatgcgc catctattga aacagtcccg ttccagggtt ctgatcccct
     2521 tgctgtcagc aaagagatcc ggcagtatct gacggtccat ccggatacct atgccattta
     2581 tacctgcagt gcccgtttta cctaccatat ttcccaatgc atccgacagt taggaatcca
     2641 ggaccgtatc caggtcatag gcaacgatct tttcacagaa agccgtcagg cgctgtccga
     2701 tggcatcctg cgtggagtga tcgacaaaaa aatctccaag caaagcgctc ttgccgtaaa
     2761 gaccctgttt gattatctgc taaaaaagga ttatccccgc agcagctgtc tggtcatgga
     2821 accggaaata gtgttaagga gcaatttcca aagccattca gacttgcaaa aataaacgta
     2881 ccgactataa aaaaacggtt atttccacgg cagatgactg cacaggaaat aaccgttttc
     2941 tcatatttta tttttctcag tttttatact tacagaagtg tttctacgta agccttgatc
     3001 gcctcatcct tgcaatttgc aaagaacagt tccttaaact ttggtccggc tacagcgccc
     3061 tttaccaatt cctgatccag tcccttgcag caggtgatca catctttaaa gttgttggct
     3121 ctcacctgat ccaggatctt cttgtttctc tgctccggta ctacacgctc cggcggatag
     3181 ccgttaccgc tgccaaaacc aaataatttc tcaaaactgt aacgaaggtt cagctcaccg
     3241 ccccatccgt aattctgtgc atatggcata gctacgcagt ttccgtcatt cacctgtgca
     3301 aacagaaatg catctgatgg attggatacg tgtccacaca ctacatttgg aaaactgttg
     3361 caggcgatca tagctccctg accagtacca cagcctgtca ctacaaaatc tgcagctcct
     3421 gaagaaagaa gcaaggaagc cagaataccg cactgcacat aggtaagctg tgcctcgtca
     3481 tctgcggaat acatgccgta gttgtctact acatgtccca ttggttccac tactccctta
     3541 agttcacggt aaatgatctc gtttttgcca gcctggctgt tttcgttgat caacgcaatt
     3601 ctcattattt tatcctcctg atccctaaaa tacattttca cctataactg cgtcctctga
     3661 cgcagccata tttttctcat cttgatctta atcttacaga aaatcttctc tcataggagt
     3721 aaagatatcg atcaaaatac catcttccag acaggtacag ccatgcatca ctccgtcctg
     3781 cttcagcagc gtatcccctt tactgactgt atgtacttct ccgtcaatgg taaattcaaa
     3841 cttaccggat gccacatagg tgatctgggt atgtggatgg ttgtgcattg ctccgatgga
     3901 acctttctta aaggtatttt ccacgcacat aacattatca caataagcta agacccg
//
LOCUS       DBMDUA010000229         3955 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8218_length_3955_cov_3.444473, whole genome shotgun sequence.
ACCESSION   DBMDUA010000229 DBMDUA010000000
VERSION     DBMDUA010000229.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8218_length_3955_cov_3.444473"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            217..1452
                     /locus_tag="ACZPCL_12465"
     CDS             217..1452
                     /locus_tag="ACZPCL_12465"
                     /inference="COORDINATES: protein motif:HMM:NF014564.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR1163317.1"
                     /translation="MEQYKPLKGVKVVELSLMVASASCGRMMADWGADVIKLENTKGG
                     DNFRKWPLSIGAPADDDFDPIFDNLNANKRAVSIDTRTKEGQEIVYRLLEDADVFLTN
                     LRTEALRKQGLDYDTLKEKFPKLVMCQLDGYGPKGEEAGRPGYDNTAFWARGGFMYSQ
                     AVYGDSNDTYPVYMPMGFGDTTCALGMMAATVSAVLSARQTGKGDRVSLSLYGTAAWL
                     ANILVSGSQYGFKMPKRRENSSPFGAPFKCSDGRWFMPQVVVFNRDTPIFYHLLGADD
                     MIGDPRYATRAQFNDVNICRPVIERFEKIFATKTAKEWYDMFSAEGLCCEILARYEDV
                     LTDPQAIANDFVYKMKYDNGQEATLVRTCLRSERMGTPEFNRGPMLGEHTVSILKEAG
                     YSDEEIEKYLSEGIIRQHD"
     gene            complement(1532..2392)
                     /locus_tag="ACZPCL_12470"
     CDS             complement(1532..2392)
                     /locus_tag="ACZPCL_12470"
                     /inference="COORDINATES: protein motif:HMM:NF046782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR1163318.1"
                     /translation="MLDESAVGLSVEQDFSYTWRDLILYNLSVGAKQEELEYVYEKGL
                     KAVPTFGVIPCTATFGTEPYSEQPLMPTKKIEGLRSDGTLHMDHKLVIHKPISKEGKL
                     HLEKVISAVYDRGEGKGAKINVDIIAKDEAGDPVFTNTMGYLNRWAGGFGGPKVPHST
                     VVIPEREADYEIKDAYPENAPLLYRLTGDTYPLHADPAFAKKAGFDRPIVHGLCSLGY
                     ACRMMIDALFPGEPERMVSIENQFRAVALPGDHFTLHVWKTGENEALFRMVKDSDGKA
                     ILDYGRMTWK"
     gene            complement(2490..3716)
                     /locus_tag="ACZPCL_12475"
     CDS             complement(2490..3716)
                     /locus_tag="ACZPCL_12475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR1163319.1"
                     /translation="MLDTAAAGLVLTGEDKEIVDMVHDFVLKEVRPKAAEHDISGELD
                     WDAYNKAFEMGLVCADLPAEYGGQGLSNVSAAWIREELMYGDAGFGLTCGTNNLGIKP
                     VLIAGTEEQKKWCVEILTSDKPGRDPRWPKKRSGFAAFALTEPEAGSDAGACKTTADK
                     ILDEDGNVKEYVLNGRKCFITNACYADFMCVVASIDRSLGYKGLTMFLVDAHLPGISI
                     GKHEDKMGIRQSATCDVLFEDVHIPASALIGKEGEGFKIAMKTLEQGRAGVGSGCVGI
                     MKAAMEVCVKYAQERTTMGKPIYKNQAISSKIADMEIAIETSRAIGMKVAALLDAGDP
                     LAATLGPIAKCYCSDALNRVTTEAVQILGGYGYMRDYPVEKLMRDAKIFQIFEGTNEI
                     QRVVISGNVIRKNKIK"
BASE COUNT         1036 a          988 c          790 g         1141 t
ORIGIN      
        1 atactcaggt taagtatcct tgcaagttcg gatattgaaa cagacttcac ctcattcagt
       61 gtataccata ttttttcagc attgattttt acagatttct cacccatgct ctctttactt
      121 tcgtaatcca ttccatagga tttctctttt tggattgata ttatatagac aaagaaacaa
      181 actactaaat aaaattaatg taacggagga atgcatatgg aacaatataa accattaaaa
      241 ggcgtcaaag tggtggaatt atctcttatg gtagcttctg ccagctgcgg acgtatgatg
      301 gctgactggg gtgcagacgt tattaaactg gaaaacacaa aaggcggtga caatttccgt
      361 aaatggccgc tgtccatcgg tgctcctgca gatgatgatt tcgacccgat ctttgacaac
      421 ttaaacgcca acaagcgtgc tgtcagcatt gacaccagaa ccaaagaggg ccaggagatc
      481 gtataccgcc tgttagaaga tgcagatgta ttcttaacca acttaagaac agaagcactg
      541 cgcaaacagg gccttgatta tgatacttta aaagaaaaat tcccaaaact ggttatgtgt
      601 cagttagacg gctatggtcc aaagggtgaa gaagccggaa gacctggtta tgacaacact
      661 gcattctggg caagaggcgg ctttatgtac agccaggctg tatatggaga cagcaatgat
      721 acctatcctg tttacatgcc tatgggcttt ggtgatacca cctgtgccct tggtatgatg
      781 gctgctactg tttccgctgt tttaagcgca agacagaccg gaaaaggtga ccgcgtttcc
      841 ttaagccttt acggaactgc agcatggctg gcaaacatcc ttgtttccgg ttcccagtat
      901 ggttttaaaa tgccaaaacg cagagaaaat tccagtccct ttggtgctcc tttcaaatgc
      961 agtgacggcc gctggttcat gcctcaggtt gttgtattta acagggatac tcctattttc
     1021 tatcaccttc ttggtgcaga cgatatgatc ggtgatcccc gttatgctac cagggcacag
     1081 tttaatgatg taaatatctg ccgtcctgtt atcgaacgtt ttgagaagat attcgctaca
     1141 aaaactgcca aagaatggta tgacatgttc agcgcagaag gcctctgctg cgagatctta
     1201 gcccgttacg aagatgtcct tacggatcca caggcaattg ccaatgattt cgtctacaag
     1261 atgaaatacg acaatggcca ggaagcaacc ctggtacgca cctgcctgcg cagcgaacgc
     1321 atgggaaccc cggaatttaa ccgcggaccg atgcttggtg aacacactgt atctatcctg
     1381 aaagaagccg gatattctga tgaagaaatt gagaaatatc tgtctgaagg aatcatccgc
     1441 cagcatgact aatctgttta tccatagaaa ataactttaa agggagcgcc ggttcaaccg
     1501 gcagccccct tttttagttt gccatgttct tttatttcca ggtcatccgt ccataatcca
     1561 gaatcgcttt tccatcgctg tcctttacca tacggaataa tgcttcattt tcccctgttt
     1621 tccatacatg aagggtaaaa tgatctcccg gcagtgctac tgcgcggaac tggttctcaa
     1681 tggataccat gcgctccggc tctcctggga ataatgcatc gatcatcatg cggcaggcat
     1741 agcctaagct gcaaagtcca tgaacaatag gacggtcaaa accggctttt tttgcaaatg
     1801 caggatctgc atggagcggg tatgtatcac ctgtcagacg atacagtaac ggcgcattct
     1861 ccggatatgc atcctttatc tcatagtcag cttctctctc cgggatcact acggtgctgt
     1921 gaggtacctt cggcccgcca aaaccaccgg cccagcggtt taaataaccc attgtattgg
     1981 taaatacagg atctcccgcc tcatcttttg caataatatc tacgttgatc ttggctcctt
     2041 ttccttctcc ccggtcatat acggcgctga tcaccttttc cagatgaagt tttccttctt
     2101 ttgagatagg cttgtggatc accagcttat gatccatatg taaggtgccg tcagaacgaa
     2161 gtccttcgat cttctttgta ggcattaacg gctgctctga atatggttct gttccaaagg
     2221 tagctgtaca tgggatcaca ccaaaggtag gtactgcctt caagcccttt tcatacacat
     2281 attccagctc ttcctgcttt gccccaacac ttaaattata aaggatcaga tctctccagg
     2341 tataggaaaa atcctgctct acggataaac caactgcaga ctcatctaac ataaaagtaa
     2401 cctcctcatc ttcctctgaa cataaaggct gctgcaaaac atttttccta ttttgcggca
     2461 gcctcacttt catctatgta tcctgagcat tatttaattt tgttcttacg gatcacattg
     2521 ccgctgataa ctactctctg gatctcattg gttccttcaa agatctggaa gatcttcgca
     2581 tcacgcatca gcttctcaac cgggtaatca cgcatatatc cgtatccgcc taagatctgg
     2641 actgcttctg tagtaacgcg gtttaatgca tctgagcagt agcacttagc gatcgggcct
     2701 aaagttgctg ctaaaggatc acctgcatct aacagtgctg ctaccttcat accgatagca
     2761 cggctggtct caatggcgat ctccatatct gcgatcttgg aggaaatagc ctggttcttg
     2821 taaattggct tgcccatggt agtacgctcc tgggcatatt ttacgcatac ttccatagct
     2881 gccttcataa tgcctacaca gccgcttcct acgcctgcac ggccctgctc cagagtcttc
     2941 atggcaattt taaagccttc gccctctttg ccaatcagtg cagatgcagg gatatgtaca
     3001 tcttcaaata atacatcaca ggtagcagac tgacggatac ccatcttatc ttcatgcttg
     3061 ccaatgctga tgcctggcag atgtgcatct actaagaaca tggtcagtcc cttataccca
     3121 agacttctgt caatagaagc aactacacac ataaagtctg catagcatgc gttggtgata
     3181 aagcacttac gtccgtttaa aacatactct tttacatttc catcttcatc taaaatctta
     3241 tcagctgtag tcttgcatgc acctgcatcg gatcctgctt ccggctcagt cagagcaaaa
     3301 gctgcaaatc cgctgcgttt ctttggccag cgtggatctc ttcccggctt atcgcttgtt
     3361 aaaatttcta cacaccattt tttctgctct tccgttcccg cgatcagaac cggcttgata
     3421 cccagattgt tggttccaca tgttaatcca aaacctgcat caccatacat cagctcctca
     3481 cggatccatg ctgcagatac attgctcagt ccctggccgc cgtactctgc cggcagatct
     3541 gcacatacaa gacccatttc aaatgccttg ttgtaagcat cccagtccag ttcaccggaa
     3601 atatcgtgct ctgctgcctt tggacgaacc tcctttaaaa cgaagtcatg gaccatatcc
     3661 acgatttcct tatcctcacc tgttaatacc aatcctgctg ccgctgtatc cagcatacga
     3721 ttaccacctt tctccttgaa tattttatgg actccaaaaa cccggttata aactgcgttc
     3781 caggagcatg tttgtatttc atatctctat gtgaatataa taacagattc ccttcattcc
     3841 tcacacgttt acacctacaa aaaagggggg tttcaaaata tgctgttgtg gtcaaaataa
     3901 ccatgaaatt ttgcacagtt tctgctatac tgtatttgtt gtattctgtc ctcat
//
LOCUS       DBMDUA010000230         3915 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8297_length_3915_cov_2.294966, whole genome shotgun sequence.
ACCESSION   DBMDUA010000230 DBMDUA010000000
VERSION     DBMDUA010000230.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8297_length_3915_cov_2.294966"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            274..2496
                     /locus_tag="ACZPCL_12480"
     CDS             274..2496
                     /locus_tag="ACZPCL_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1163320.1"
                     /translation="MEKNENCKENRFLSMDSLKKNSRFYLVAAFLAAAILALLCVGNG
                     IWKEYRNSAVRNQKDQMLLAVESLSGRLEETISEYASDIRSMWSCSHTLNEQERETLW
                     DTYVKEHGPVVQDVIIKKKGETILTSGTDNEPEEILSESKIDDQMCFRLVKIGNESHY
                     LMLHYETDTGETISMILNGMAYYNKTIRPLNVGTNGYFILKDHNGIVLMHPQLEQWGI
                     DVINGREKMFPGKNLTSLSNMIDRQKQGLTAVDEYYSYWWTKPGAPGVEKVSAYTPAY
                     IGDDFIIVSAVMDQSDMYVPLEKGALKLLFVFTGVLFTILAAASYIFHLMLKSRKDRQ
                     QISYLTELNGILERMHQSEETIAHQQRLQIMGTMTGGIAHEFNNLLTPIMGYAEFLMM
                     DLPEGSDNYDSAKEIYDASVKAKEIIQQISSLSRKNMETAFKTLDADRVFKRALKMVR
                     SVCPDNIELKENINLPGVTIVGNETQLNQVILNIGVNAIHAIGHETGEIEVSAILLDK
                     KDLDPDLPIGNENAWDQYVRIDIRDNGCGMSPDVLKQIFDPFFTTKKGGKGTGLGLAL
                     TEQIVTSHRGVVNAESAPGVGSVFHLYFPASVPDGNSSFAPSVQDTLDSVRKNLSDQL
                     GEKISLLIVDDNPKVLRLLERNFSKRDIKVTCVMNFKEATEKLKTDRFDALAVEQFID
                     GNSAVDFCMSVKGRYNGMLRLIMANQVDRELAEAKEKNIIDNYMIKPVSDLDILNEMR
                     "
     gene            complement(2725..>3915)
                     /gene="citF"
                     /locus_tag="ACZPCL_12485"
     CDS             complement(2725..>3915)
                     /gene="citF"
                     /locus_tag="ACZPCL_12485"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HIR1163321.1"
                     /translation="IQSSGVRGKIGEAISAGKLKGLAIMRSHGGRVRAIESGETRIDI
                     AFIGTPTCDDYGNCRGIGGKSDCGVLSYAMVDGDYADKVVAITDCLVPFPNFPAHISM
                     TKVDYVVVVDAIGDPKKIATGAAKPTTDMRKLMMADYCTQFVVNSPYFKDGFSYQTGV
                     GGASIASTISLAKIMKERNIRMRFGVGGLTKPMCDLLINNQVDCLLDTQDFDLAAVES
                     VKNLKHFRISAGEYADPFNKGAVVNKLDFVILAALEVDVNFNCNVVVGSDGVITGAQG
                     GHPDTAAGAKCTIVIAPLLQGRIPAICSEVTTVTTPGESIDVVITDYGIAINPRRQDL
                     IEAMKGVDLPFKTIEELRDIAYSIVGEPEKVQFGDRVVGIIEARDGTIMDVVRQIKPY
                     EFAD"
BASE COUNT         1114 a          877 c          865 g         1059 t
ORIGIN      
        1 cactgtattg ttttttgagg atcagaaggt tgtgaaaggg cttcttttcc gcttcctgcc
       61 acttctgtat caagttcatt gctgtgcatt actttttcaa gtaatttaca aatagaaatg
      121 tcgtcatcaa cgatcagtac tttatctgcc atgtttcttt tcctattctt gtgtgatttc
      181 gtggaattat cataaaactt ttgcatatat gcattaaatt cattataata ttaccaaaga
      241 agcaagtcaa cctgaaagga catgatcgct tatatggaaa agaatgaaaa ctgcaaagaa
      301 aatcgtttcc tgtctatgga ctcattaaag aaaaacagcc gcttctatct ggttgcggca
      361 tttttagctg ctgctatcct ggcactttta tgcgtcggca acgggatctg gaaagaatac
      421 cgcaactccg ctgtgcgcaa tcagaaagat cagatgcttt tagcagtgga aagcctttcc
      481 ggacgtctgg aagaaaccat cagcgaatat gcatctgaca taagaagcat gtggagctgc
      541 agccatactt taaacgaaca ggaacgagaa acgctctggg atacttatgt aaaagagcat
      601 ggtcctgtag tccaggatgt tatcatcaag aaaaaaggag aaaccatcct gacatccggt
      661 acagacaatg agccggaaga gatactttct gagtcaaaaa tcgatgatca gatgtgtttc
      721 agactggtaa agattggaaa tgaaagtcac tatcttatgc tccactacga aaccgacacc
      781 ggagaaacca tttccatgat cttaaatggc atggcctatt acaataagac catacgcccc
      841 ttaaatgtag gaaccaacgg ttatttcata ttaaaagacc acaatggcat cgtactgatg
      901 catccacagc tggagcaatg gggaattgat gtaataaacg gacgtgaaaa aatgtttccc
      961 ggaaaaaatc tcaccagtct cagcaatatg atcgaccggc aaaaacaggg actgacggct
     1021 gtggatgaat attattccta ctggtggacc aaaccaggtg ccccgggagt ggagaaagtc
     1081 agcgcctata ccccggccta tattggggat gattttatca ttgtcagcgc tgttatggac
     1141 cagagtgata tgtatgtacc tttggaaaaa ggcgccttaa aactgctttt tgtatttaca
     1201 ggtgttcttt ttaccattct tgctgctgct tcctatattt tccatctgat gttaaagagc
     1261 agaaaggacc gtcagcagat ctcttattta acagaattaa atggaatctt agagcgaatg
     1321 caccaaagtg aggaaaccat tgcccaccag cagcgtttac agatcatggg aaccatgacc
     1381 ggcggtattg cccatgaatt taataacctg ctgacaccca tcatgggcta tgcggaattt
     1441 ctcatgatgg atcttccgga aggctccgac aactatgaca gtgccaaaga aatttatgat
     1501 gcttctgtaa aagccaagga gatcatacag cagatctctt ctctcagccg caaaaatatg
     1561 gaaactgcct ttaagacttt agacgctgac cgggtattta aacgtgcttt gaaaatggtc
     1621 cgctccgtat gtccggataa tattgaactg aaagaaaata ttaaccttcc aggtgttacc
     1681 atcgtaggaa acgagaccca gttaaatcag gtgatcttaa atattggcgt caatgccatc
     1741 catgccattg gacatgaaac cggtgagatt gaggtatctg ccattctgtt agataaaaag
     1801 gatctggatc cggatcttcc tattggaaat gaaaatgcct gggatcagta tgtgcgcatt
     1861 gatatacggg acaatggctg cggtatgagt ccggatgtat taaagcagat ctttgatcct
     1921 ttctttacta ctaaaaaagg tggaaaagga acaggacttg ggctggccct tacagaacag
     1981 atcgtcactt ctcacagagg cgtggtaaat gcggaaagcg cacctggcgt cggttctgta
     2041 ttccatctgt actttcctgc atctgtacca gatggaaaca gcagctttgc cccgtctgtc
     2101 caggatacgc tggatagtgt gagaaaaaat ctctcagatc aattaggaga aaaaatctca
     2161 ttgctgatcg tggatgataa tcctaaggta ctccgtctct tagaacgcaa cttctcaaaa
     2221 cgggatataa aagtcacctg cgtgatgaac tttaaagaag cgacagaaaa attaaaaaca
     2281 gaccgctttg atgctcttgc tgtagaacag ttcattgatg ggaattccgc tgtagatttc
     2341 tgtatgtctg ttaagggaag atataatgga atgctccgcc tgatcatggc caaccaggta
     2401 gaccgggaac tggcagaggc gaaagagaag aatatcattg ataactatat gatcaaacct
     2461 gtgtcggatc tggatatttt gaatgagatg agataaattc tcatggtttg aaattttcaa
     2521 atggaaaagg cccatgccgc taccgtaccg gcagcctgca tgggccttgt tttgtagatt
     2581 tataaatttt gcagactcac aaacttctca gactcacaaa cccgcgctta cgcgctccac
     2641 tgcctgctta cgcaggcggt ttgttcgttg ctgacacgca ccaaaaacaa atgtctgctg
     2701 cgcagccgct tgtttttggt ttgcttagtc agcaaactca tatggcttga tctggcgtac
     2761 aacgtccatg atcgtgccat cgcgggcttc gatgatacct acaactctgt ctccaaactg
     2821 aaccttctcc ggctcgccta cgatagaata tgcaatgtca cgcagttctt caatggtctt
     2881 aaatggcagg tctacgccct tcattgcctc gatcagatcc tgtcttcttg ggttgatagc
     2941 aataccgtaa tccgtgataa ctacgtcaat gctctctcca ggagtggtta ctgtggttac
     3001 ctcgctgcag atcgcaggaa ttcttccctg aagcagtgga gcgataacga tagtacactt
     3061 agctcctgct gctgtatcag gatgtccgcc ctgtgcaccg gtaatgacac cgtcagaacc
     3121 tacaactacg ttacagttaa agtttacgtc tacttccagt gcagccaaga ttacgaaatc
     3181 cagcttattt acaacagcac ccttgttaaa tgggtctgcg tactcaccag cagagatacg
     3241 gaaatgcttt aagttcttta cagactctac tgctgccagg tcgaagtcct gagtatcaag
     3301 caggcagtct acctggttgt tgatcagcag gtcacacatt ggcttggtca gaccacctac
     3361 gccaaaacgc atgcggatat ttctttcctt catgatctta gccagagaga tagtagatgc
     3421 gatagaagca ccgcctacac ctgtctggta ggagaagccg tccttgaaat atgggctgtt
     3481 tactacgaac tgggtacagt aatcagccat catcagcttt ctcatatcgg tagttggctt
     3541 tgctgcacct gttgcgatct tctttggatc accgatagca tctacaacta ctacatagtc
     3601 aaccttggtc atggagatat gggccgggaa gttcgggaat ggtaccaggc agtcggtaat
     3661 agctactacc ttatctgcat agtcaccatc caccatagcg taggaaagca cgccacagtc
     3721 actcttacca ccgataccac ggcagttgcc atagtcatca caggttggtg ttccgataaa
     3781 tgcgatatca attcttgtct caccgctctc gattgctctt acacggccac cgtgggaacg
     3841 catgattgca agtcccttta atttacctgc agagattgct tcaccgatct taccgcggac
     3901 accggaggac tggat
//
LOCUS       DBMDUA010000231         3887 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8374_length_3887_cov_2.947966, whole genome shotgun sequence.
ACCESSION   DBMDUA010000231 DBMDUA010000000
VERSION     DBMDUA010000231.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8374_length_3887_cov_2.947966"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="ACZPCL_12490"
     CDS             <1..244
                     /locus_tag="ACZPCL_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1163322.1"
                     /translation="HVRLSPTYVCTLFKNETGQTLNQYLTDYRIKMSKQFLSDPLYKI
                     TDISAKVGYSDGNYYSKAFRKIVGLSPSEYREKMLR"
     gene            241..2079
                     /locus_tag="ACZPCL_12495"
     CDS             241..2079
                     /locus_tag="ACZPCL_12495"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1163323.1"
                     /translation="MNFFNRILHYYIYDMRLKKKLVVSHTILFLIPTAVLICFLFLNI
                     FRIVMDDTIRSEQALSSQSVLSVENLVSHVTHASDTLTSSYVIRDMFDISNAQAQGLI
                     PSRSKISNLMRLTDTLTDHSLIESVRFYYDDHTYHRLEQFNTNAQRLFVPLSEVSSPW
                     LEYFNAEGWSKFFFPKSALSEKEADENGPLAYIKPIFYRSNEEDVPKIAAYAAIYFSE
                     DAFEEILKKDSSLKGEFSFLINDDLDLVAASDISLFSGNFAITAFESDPDASGQFQLV
                     NYEDFSAYTACFSLKNTDWYMVSLIPRDQIAGIGHSVVLNFAFTYGMIALFALFIAYK
                     LSESIADRIIGVALQMETIRKGRPEPLELTAAGNDEIGVLSGTYNYMTAEINQLMDYE
                     EKAATELRIAEFRALQAQINPHFLYNTLDMINWLSQSGQSEKVTEAVQALTRFYRLTL
                     GSKDLISTVQDEVTHVSLYMQLQNMRYNNCAEFIADVPPELDHFSIPRLTFQPIVENA
                     LLHGIMMKEEKKGTILLTGWREENDIVFVISDDGAGIPPEKLDSLLDERTQAAVGSSS
                     RHVGVSNTNLRLKSLYGSGYGLSFTSVPGQGTEVTVRIPAQEEEEF"
     gene            complement(2084..2437)
                     /locus_tag="ACZPCL_12500"
     CDS             complement(2084..2437)
                     /locus_tag="ACZPCL_12500"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HIR1163324.1"
                     /translation="MDFYKRLGIVCRAVPEGKVITYGQAALLCGYPKNARQVGYGLKK
                     ELAGDVPAYKVVNSQGVLSGAASFEHPGLQRILLEEEGIEVSMDNKVDLKKYGWKNSL
                     EDAEFFRETFRRKGI"
     gene            complement(2545..2628)
                     /locus_tag="ACZPCL_12505"
     tRNA            complement(2545..2628)
                     /locus_tag="ACZPCL_12505"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2592..2594),aa:Leu,seq:cag)
     gene            complement(2669..2752)
                     /locus_tag="ACZPCL_12510"
     tRNA            complement(2669..2752)
                     /locus_tag="ACZPCL_12510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2716..2718),aa:Leu,seq:aag)
     gene            2927..>3887
                     /locus_tag="ACZPCL_12515"
     CDS             2927..>3887
                     /locus_tag="ACZPCL_12515"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HIR1163325.1"
                     /translation="MDQKILWAVNKMPKTDDKNLPIMGLEEIKKARTFHESFPQYSET
                     PLTKLPHMASYLGVKEVYVKDESYRFGLNAFKVLGGSFAMARYIAKETGKDVSELPYS
                     VLTSEQLRKEFGQATFFTATDGNHGRGVAWAANRLGQKAVVHMPKGTTQPRLQNIAKE
                     GAQVDIQELNYDDCVRLAAKEADETPRGVIVQDTAWDGYEEIPAWIMQGYGTMAMEAG
                     EQLKAQGCERPTHIFVQAGVGSLAGAVVGYFSNLYADNLPTFVVVEAEAAACLYKGAA
                     AGDGDIRIVDGDMQTIMAGLACGEPNTISWDILKNHVKVFIAAP"
BASE COUNT         1028 a          951 c          817 g         1091 t
ORIGIN      
        1 acatgtacgc ctgtctccta cctatgtgtg taccctgttt aaaaatgaaa ccggtcagac
       61 cttaaaccag tatcttacag attaccgcat aaaaatgtcc aaacagtttt taagtgatcc
      121 cctttataag atcacagaca tttccgcaaa agtaggctac agcgacggaa attattacag
      181 caaggccttc cgcaagatcg tagggctctc accatctgaa tacagggaga aaatgcttcg
      241 atgaattttt ttaaccgtat ccttcattat tatatctatg acatgcgttt aaagaaaaag
      301 ctggtggtat cccataccat cctttttctt attcctacgg ctgttctgat ctgttttctt
      361 ttcttaaata tcttccggat cgttatggat gataccatcc gctccgaaca ggccctcagt
      421 tcccagtccg tactttctgt tgaaaacctt gtttcccatg tgacccatgc ttctgatacc
      481 ctgacttctt cctatgtgat ccgggatatg tttgatattt ccaatgccca ggcccagggg
      541 ctgatcccat ccagaagcaa gatcagcaac ctgatgcgtt taacagatac acttaccgat
      601 cattctctca ttgagtctgt ccggttttat tatgatgacc atacctatca ccgcctggag
      661 cagtttaata ccaatgccca aagactgttt gtccctcttt ccgaagtttc cagtccctgg
      721 ctagaatact ttaatgcaga aggctggtca aagttctttt ttccaaaaag tgccctgtct
      781 gaaaaagagg cagatgaaaa tggtccttta gcctatatca agcctatttt ttaccgttcc
      841 aacgaagaag atgtcccaaa gatcgctgcc tatgcagcca tctatttttc cgaagatgcc
      901 tttgaagaga tcctgaaaaa agactcttcc cttaaagggg aattttcttt tctgatcaat
      961 gatgatctgg acctggtagc cgcttccgac atttctcttt tttcaggtaa ctttgcaatt
     1021 accgcttttg agtcagaccc ggatgccagc ggacagtttc agttagtgaa ctacgaggat
     1081 ttttctgcct atacagcctg tttctcttta aaaaatacag actggtacat ggtttccctg
     1141 atccccaggg accagattgc agggatcggt cactccgttg tccttaactt tgcctttacc
     1201 tatggtatga tcgccctgtt tgcccttttt atcgcctata aactgtctga gtctattgca
     1261 gaccgtatta ttggcgtagc cctgcagatg gagaccatac gaaaaggacg gccggagcct
     1321 ttagaactga cagctgcggg aaatgacgag atcggtgttc tttcaggaac ctataattat
     1381 atgactgcgg agatcaatca attgatggat tatgaagaaa aagcagccac cgaactgcga
     1441 atcgcggaat ttcgcgccct ccaggcacag atcaatcccc attttctcta taacaccctg
     1501 gatatgatca actggctgtc ccagtccggg caaagcgaaa aagtaacgga agccgtccag
     1561 gctcttaccc gtttctaccg cctgacttta ggaagcaaag acctgatcag caccgtccag
     1621 gacgaagtga cccatgtttc cctttatatg cagcttcaga acatgcgcta caacaactgt
     1681 gcggaattta ttgcggacgt tccgccggag cttgaccatt tttccatccc gcggcttacc
     1741 tttcagccga tcgtggaaaa cgccctcctt catggcatta tgatgaaaga agaaaaaaaa
     1801 ggcaccatcc ttcttacagg ctggcgcgaa gaaaatgata ttgtttttgt aatttcggat
     1861 gatggggccg gtattcctcc ggaaaagctg gactctcttt tagatgaacg cacccaggca
     1921 gccgtaggct cctcttcccg ccatgtaggc gtatccaata ccaacctgcg tcttaaaagc
     1981 ctgtacggca gcggatacgg cctgtctttc accagtgttc ccggacaggg cacagaggtg
     2041 acggtacgta taccggccca ggaggaagaa gaattttaaa taactagatc cccttcctgc
     2101 gaaatgtttc ccggaaaaat tctgcatctt ccagggaatt tttccatcca tatttcttta
     2161 gatctacttt gttgtccata gagacttcta tcccttcttc ttccaggagt atccgctgaa
     2221 gacccggatg ctcaaaagaa gcggcaccgg aaagaactcc ctggctgttt actaccttat
     2281 aagctggcac atctcctgcc agctcttttt ttaacccata acccacctgt ctggcattct
     2341 ttggataacc gcacaaaaga gctgcctgac cataggtaat gacttttccc tccggcactg
     2401 cccggcatac gatccccaga cgtttgtaaa aatccatttt tcattcctct ttccaatccc
     2461 cgtgccgaaa gccaacacaa gattgcactc atcatttttg caaaagaaaa acaggaacct
     2521 gtttccaagt tcctgtttaa atcctgcaga agaagggagt cgaaccctca aggtattact
     2581 accacacgga cctgaaccgt gcgcgtctgc caattccgcc acttctgcat ttgcttattt
     2641 agttaagcac ggtacttttt agtaccagtg cgggagatgg gacttgaacc cacacgatca
     2701 tgcaaccaca agatccttag tcttgcctgt ctgccaattc cagcactccc gcagcacgtg
     2761 ttataatata ccataggttt ttcagtttgg caatagtttt tttgaaattt ttttaatttt
     2821 tttcaaattt atttttcaga tgttttttgg agacattttc actcttcttt tgcaatcaat
     2881 cctattttgg actagccgga aagaaattaa attgatacaa tataaaatgg atcagaagat
     2941 tctgtgggca gtaaacaaga tgcctaaaac agatgacaaa aaccttccaa ttatgggact
     3001 tgaggagatc aaaaaggcgc gtactttcca cgagagcttt cctcagtaca gcgaaacacc
     3061 attaaccaag ctgcctcaca tggcttccta tttaggagtt aaggaagtat atgtaaagga
     3121 tgagtcttac cgttttggtc tgaacgcatt taaggtactg ggcggatctt ttgcaatggc
     3181 tcgttacatc gcaaaagaaa ccggaaagga tgtttctgaa cttccttatt ccgtacttac
     3241 ttccgaacag ttaagaaaag aattcggaca ggctactttc tttacagcaa cagacggcaa
     3301 ccacggccgc ggtgttgcat gggctgctaa ccgtcttgga cagaaggcag ttgttcatat
     3361 gcctaaggga actactcagc cccgtcttca gaatatcgca aaagaaggcg ctcaggtaga
     3421 catccaggaa ttaaactatg atgactgtgt tcgtctggct gctaaggaag cggatgaaac
     3481 tccaagaggc gttatcgtac aggatactgc ctgggacggc tacgaagaga tcccggcatg
     3541 gatcatgcag ggatacggca ccatggcaat ggaagctggt gaacagttaa aagctcaggg
     3601 atgtgagcgt cctacccaca tcttcgtaca ggccggtgtt ggttccctag ccggtgcagt
     3661 tgttggttac ttctccaatc tgtatgctga caacttacct acattcgtag ttgttgaggc
     3721 tgaagctgct gcatgtcttt acaagggtgc tgctgccggt gatggagaca tccgcattgt
     3781 agacggcgat atgcagacca tcatggcagg tcttgcatgt ggtgaaccga acaccatttc
     3841 ctgggacatc ttaaagaacc atgtaaaagt attcattgct gctcctg
//
LOCUS       DBMDUA010000232         3881 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8388_length_3881_cov_1.992593, whole genome shotgun sequence.
ACCESSION   DBMDUA010000232 DBMDUA010000000
VERSION     DBMDUA010000232.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8388_length_3881_cov_1.992593"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="ACZPCL_12520"
     CDS             complement(<1..115)
                     /locus_tag="ACZPCL_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013633.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HIR1163326.1"
                     /translation="MAEKKKKASNSSMRLDKFLVEMGKGSRSQIKEMAKKGR"
     gene            complement(133..1536)
                     /locus_tag="ACZPCL_12525"
     CDS             complement(133..1536)
                     /locus_tag="ACZPCL_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HIR1163327.1"
                     /translation="MTDVKQLPEAFLKEMEQLLGDEYEAYIKSYEEAWKPGLRVNTLK
                     IMPEEFKNLSHLELTPVDWTEKGFYYEDTERPARHPFYYAGLYYLQEPSAMAPAAVLP
                     VEPGDKVLDVCAAPGGKSTELAAKLQGKGMLVSNDISYSRARALLKNLELAGAENISV
                     LSEEPGKLAAVWPEFFDKILVDAPCSGEGMFRREEDMVKDWVSRGPSYYSPIQREILE
                     QAVAMLKPGGMMLYSTCTFSKKEDEENVAYILEKYPSMELVPIDKEQFKGLSDGFGYP
                     QTARLFPHRIQGEGHFLALFHKKEDENGSGSGAAVLRLSGDEKEASKRAKEAGKQEDL
                     MKFLKNCRKEWDLGRIYIRDEQVYYLPEGLKTGLPLRFLRTGLHLGELKKGRFEPSQA
                     FAMALKKDEYQVRLDLKTEDERVIRYLKGETISLVNEEEPVKGWCLVTVEGFPLGWAK
                     GNGMTLKNKYYAGWRWQ"
     gene            1630..2604
                     /locus_tag="ACZPCL_12530"
     CDS             1630..2604
                     /locus_tag="ACZPCL_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HIR1163328.1"
                     /translation="MKRILLSLLALFFLILLLLSPGTALEGARKGLLLWAYTVLPTLL
                     PFMIGTGVIAAMGAVGLVVKPFSPLLSGVFHFSQPAGFVFLTGLLCGYPMGAKMDRDL
                     LERGQISWEEACYLLSICNHPSPMFLTGYTVMEGQKLLPPSIKFPLFPFLAALYLPVF
                     PISYLARHYYDKKSKKNRKIRPSVWSGVSHTAPISSFSLDEHLMSCLETMEKIGLYIM
                     LFSILSLYLFKLPLPGPVILKPALMGLLEITTGIQTICEHTSGLTGLLLVTASVAFGG
                     ISGIFQTRSVLTMHSGRKNAGLSIRHYIFWKALHSLLSCVFFWILLKI"
     gene            complement(2619..3314)
                     /locus_tag="ACZPCL_12535"
     CDS             complement(2619..3314)
                     /locus_tag="ACZPCL_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HIR1163329.1"
                     /translation="MSRIEQLISEIEEYIDSCKFQALSNSKIIVNKEEMEELLVELRM
                     RIPDEIKKYQKIISQQETILSDAQAQSESMLADAKKQSDSMIAQATEQANTMVEQATE
                     QANSMVAQADAQTNEMVNDHEIMQRAYAHANEVVEQANVQAQAIVDAAVNDANNIRQG
                     AIQYTDDMLRSLQNIMDHTMETAKGRFDSFMSSMQSSYDIVTNNRKELSGGLVQQEPE
                     AQAAPQEEKKEEA"
     gene            complement(3359..3850)
                     /gene="coaD"
                     /locus_tag="ACZPCL_12540"
     CDS             complement(3359..3850)
                     /gene="coaD"
                     /locus_tag="ACZPCL_12540"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR1163330.1"
                     /translation="MRVAVYPGSFDPVTLGHIDIIQRTSKMFDKVIIGVLKNRSKSPL
                     FTAEERVELLKEVTADIPNVEVQSFQGLLIDFVRQNQANVIVRGLRAITDFEYELQMA
                     QTNRVIAPEIDTIFLTTNLKYSYLSSSIVKEIAEFNGDIKEFLHPIVVERVKEKLEEM
                     KNV"
BASE COUNT          891 a         1020 c          701 g         1269 t
ORIGIN      
        1 tccgtccttt tttcgccatt tccttgatct ggctgcggct tccttttccc atttccacca
       61 gaaatttgtc caggcgcata ctggaattag aagctttttt cttcttttct gccatatttc
      121 atttatcctc tcttactgcc agcgccagcc tgcatagtat ttatttttaa gggtcattcc
      181 attgcctttg gcccagccta aaggaaagcc ttctactgta accagacacc agccctttac
      241 cggttcttcc tcatttacaa gactgatcgt ttctcctttt aaatagcgga tcacacgttc
      301 atcctctgtt ttcagatcaa ggcgtacctg atattcgtct ttttttaatg ccattgcaaa
      361 cgcctgggat ggttcaaaac gacctttctt taactcaccc agatgcagcc ctgttcgcaa
      421 aaaccgcagc ggaagtcctg tttttaagcc ctccggcaga taatatacct gctcatcccg
      481 gatatagata cggcctaaat cccattcctt cctgcagttt ttcagaaatt tcatcagatc
      541 ttcctgtttg cctgcttcct tggcccgctt tgaagcctct ttttcatctc ctgatagcct
      601 caaaacagct gcaccactgc cagaaccatt ttcatcctct ttcttgtgga ataatgccag
      661 gaaatgtcct tccccctgga tccggtgtgg gaacaggcgg gcagtctgtg gatatccaaa
      721 accatccgaa agtcccttaa actgctcttt atcaatagga accagttcca tggatggata
      781 tttttctaaa atatacgcca cattttcctc atcttctttt tttgagaagg tacaggtaga
      841 ataaagcatc attcctcctg gttttaacat ggccacagcc tgctctaaga tctccctctg
      901 gattgggctg taataagagg ggcctctgct tacccagtcc tttaccatat cctcttccct
      961 gcgaaacatt ccttccccgg aacatggtgc atctactaaa atcttatcaa aaaactccgg
     1021 ccatacagct gccagttttc ccggttcttc acttaaaaca gaaatatttt ctgctcctgc
     1081 cagttccagg tttttaagta atgctcttgc cctggaataa ctgatgtcat tggatacaag
     1141 cattcctttt ccctgaagtt ttgctgccag ttccgtactt tttcctcccg gtgccgcaca
     1201 tacgtctaag accttatctc ccggttctac cggaagcaca gccgctggtg ccatggcact
     1261 gggctcctga agataataaa gaccggcata ataaaaagga tgtctggcag gacgttctgt
     1321 gtcttcataa taaaatccct tttccgtcca gtctacaggt gtcagttcca ggtgggaaag
     1381 atttttaaat tcctccggca taattttcaa tgtatttaca cgcagacctg gtttccacgc
     1441 ttcctcatag ctttttatat atgcctcata ttcatctcct aaaagctgtt ccatttcctt
     1501 taagaacgct tccggcagct gtttcacatc tgtcatatcc tgctccttat acatgctact
     1561 acttaaacat catttttcaa aagatccggc catatattaa aacaaaccct gcatttaacc
     1621 ggaggtccta tgaaacggat cctcttaagc cttctggccc tgttctttct tatcctgctt
     1681 ttgctctctc cgggtaccgc tttggaaggt gccagaaagg gactccttct ctgggcttac
     1741 acagttttgc ctactttgct gccttttatg atcggaaccg gcgtcattgc tgccatgggc
     1801 gctgtaggtc ttgttgtaaa gcccttttct cctttattat ccggagtatt ccacttttct
     1861 cagcctgcag gcttcgtttt tctcaccgga ctgttatgcg gctatcccat gggggctaaa
     1921 atggaccggg acttattaga aaggggacag atttcctggg aagaagcctg ctatcttctc
     1981 tccatctgca atcatccaag ccctatgttc ctcaccggtt atacagtgat ggaaggacag
     2041 aagctccttc caccttccat aaaatttcct ctttttccct tccttgcagc cctttatctt
     2101 ccagtttttc ccatttctta tctggcaagg cattattatg ataaaaaatc caaaaagaac
     2161 aggaagatac gtccttctgt ctggtccggt gtctctcaca cagcacccat ttcttccttc
     2221 tccctggatg agcacctgat gagctgcctt gaaactatgg aaaaaatcgg gctttatatc
     2281 atgctgtttt ccatcttatc cctgtatctt tttaagctgc cccttcccgg ccctgttatt
     2341 ttaaaacctg ctcttatggg gcttttagag atcactaccg ggatccaaac catctgcgaa
     2401 catacatccg gtctcactgg tctgctctta gtcactgcaa gtgttgcttt tggcggtatc
     2461 agcggtattt tccagacccg cagtgttctt acaatgcaca gcggcagaaa aaatgccgga
     2521 ctctccatcc ggcattatat cttctggaaa gcccttcaca gtttactttc ctgcgtattc
     2581 ttttggattc tcttaaaaat ctgatccctt tcagaaattt aagcttcttc cttcttttct
     2641 tcctgaggtg ctgcctgtgc ttccggttcc tgctgtacaa gaccaccaga cagttcctta
     2701 cggttgttgg ttacaatgtc atagctggac tgcatggaac tcataaagga gtcaaaacgt
     2761 ccctttgcag tttccatggt atgatccata atattctgaa ggctgcgcag catatcgtct
     2821 gtatactgga tcgcaccctg gcggatattg tttgcatcat taacagctgc atctacgatt
     2881 gcctgagcct ggacatttgc ctgctcaact acttcgtttg catgtgcata agcacgctgc
     2941 atgatctcat ggtcattgac catctcgttg gtctgtgcat ccgcctgtgc taccatagaa
     3001 ttagcctgtt cggttgcctg ctctaccatg gtgtttgcct gctctgtagc ctgtgcgatc
     3061 atggagtcag actgtttctt tgcgtctgcc agcatagact cagactgagc ctgggcatca
     3121 cttaaaatgg tctcctgctg gctgatgatc ttctggtatt tcttgatctc atccgggata
     3181 cgcatccgaa gctctactaa cagctcttcc atttcttcct tattaacaat gatcttgctg
     3241 ttggacagag cctggaattt acaactgtct atgtattctt caatttcact gatcaactgt
     3301 tcaattctgc tcattttatc gttctccttt ggtttattac gtgtatctat tacatactct
     3361 atacgttttt catctcttcc agcttttctt tcacccgttc tacaacaata ggatgtaaaa
     3421 attctttaat atcgccgtta aattctgcga tttcttttac aatactggaa ctgagatagg
     3481 aatatttcaa attcgtggtt aaaaatatag tatcgatctc cggcgcgatc actcgattgg
     3541 tctgcgccat ctgaagttca tactcaaaat ctgtgatcgc tcttaatcct cttacgatca
     3601 catttgcctg attttgacgg acaaaatcga tcagaaggcc ctgaaaagac tgcacctcta
     3661 catttggaat atccgcagtc acttccttta acagctccac tctttcctct gcagtaaaca
     3721 atggtgattt ggaacggttt ttcaaaacac cgatgatcac tttgtcaaac atcttagaag
     3781 tacgctgtat aatatcaata tgtcctaatg taaccggatc aaaacttccc ggatatactg
     3841 cgactctcat ttatgccccc ttgccagcaa atatatgctt g
//
LOCUS       DBMDUA010000233         3835 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8503_length_3835_cov_2.906802, whole genome shotgun sequence.
ACCESSION   DBMDUA010000233 DBMDUA010000000
VERSION     DBMDUA010000233.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8503_length_3835_cov_2.906802"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="ACZPCL_12545"
     CDS             <1..868
                     /locus_tag="ACZPCL_12545"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1163331.1"
                     /translation="IDSISVILSEDTDDYYQNFRKGMEKAATDLRSDVRLITLYDRKD
                     QDQQVELTTREIRDGAGAVVIEPAEEKAAVMALESLNPGCPVIFVSASSPSDFVTDSI
                     LIDYFEAGRILGEKAAEKNLPGEKVCLFAQGMAYNGVQGAYEGVCSVLNDQGISYELY
                     VDKEGKAFRKVIENTVYPGTGQVEVVALDVQSLDRTASILNESSVYRDHVSALYGIGS
                     TTGILNLLDQGIIDGLVTYDQFSQGYLSIKKAVEAIHGSRQKQETLLDPVYLDQQELA
                     SGAYEKMLYPME"
     gene            882..1940
                     /locus_tag="ACZPCL_12550"
     CDS             882..1940
                     /locus_tag="ACZPCL_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1163332.1"
                     /translation="MKRTKGTGIMLFGVLAIVVIIGVFILAGNGAKKEAASKEIRIGL
                     LLYRGDDTFISTLRSYIEEKAKEYERTEGIKVTLDILDAKSNQNTQNNQVDRLLELGC
                     NALCVNIVDRFAASVVIDKAMAADVPVIFFNREPVEEDMNRWEKLYYVGADAKESAVL
                     EGKILVDAYRKDPASLDRNGDGTVSYVLLEGESNHQDSLIRTEWSIQTLKDGGVPLEK
                     ITGGIASWERSQASAMMEQWLTDYPDQIELVICNNDDMALGAIDAIERAGILPGTIKL
                     AGIDATPPGVEALKAGKLFGTVEADKEGYANAIFGIAASLSRGLPIDESIKLENGKYF
                     WCSQKGLTQADIAGTDNK"
     gene            2116..3246
                     /locus_tag="ACZPCL_12555"
     CDS             2116..3246
                     /locus_tag="ACZPCL_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009663458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1163333.1"
                     /translation="MRLVKKVTAAAMASAMALSLAACGGGNTATTAAPTEAAKETAAA
                     SEAATEAAKEAAETAANAAGTADVADKKVGISIYKFDDNFMTLYRQELQRYLTEDLGF
                     KPENVVIQDGKGDQAEQTNQINNFITQKYDVLILNLVQASSAPEITDMCKEAGIPVVY
                     INREPDEAEEQRWVDEKINATYVGCDARQSGTYQGEEILETETKGDINGDGKVSYIMI
                     QGDPENVDAQYRTEFSVKALTDAGVEVEELLKQRGDWDQAKAQQIAQDALNQYGDKIE
                     VIFCNNDAMALGALQAIEAAGRKVNEDIYLVGVDALTEAVQNVIDGKQTGTVFNDHFS
                     QAKAAADAAVQFISGEEVDAKIPVDYIKVTNANAQEILDKLK"
     gene            3367..>3835
                     /locus_tag="ACZPCL_12560"
     CDS             3367..>3835
                     /locus_tag="ACZPCL_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1163334.1"
                     /translation="MAEQYRLEMIGVSKSFPGVKALDGINLKVRPGTVHALMGENGAG
                     KSTLMKCLFGIYKMDEGQIIIDGEKTEITNPDDALRKGLAMVHQELQPIPDRSIAENM
                     YLGRYPMKSVGPLKMVDHKTMNSEAEKWLKDVKMNFDPKAKLGTLSIGQMQSVE"
BASE COUNT         1202 a          735 c         1029 g          869 t
ORIGIN      
        1 gatcgactcc atttctgtga tcttaagcga agacacggac gattattatc agaatttcag
       61 aaaaggaatg gaaaaggcag ctacggatct gcgaagtgat gtaaggctga tcactcttta
      121 tgaccgaaaa gatcaggatc agcaggtgga actgaccacc agggagatcc gggacggggc
      181 aggtgctgtg gtgatcgaac cggcagagga aaaggcggcg gttatggcgt tagaaagttt
      241 aaatccagga tgcccggtga tttttgtaag cgcgtcttcc cccagtgatt ttgtgacaga
      301 ctctattctc attgattatt ttgaagccgg aaggatattg ggagaaaaag cagcagaaaa
      361 aaaccttcca ggggaaaaag tgtgtttgtt tgcccaggga atggcttata acggcgtaca
      421 gggcgcctac gaaggggtct gctctgtttt aaatgaccag gggatctctt atgagttata
      481 tgtagataaa gaaggaaagg cttttcgcaa ggtgatcgaa aatacagtct atccggggac
      541 cggtcaggta gaagtggtgg ctctggatgt gcagtctttg gaccggacag cttctatctt
      601 aaatgaaagc tctgtttaca gggatcatgt atctgccctt tatggcattg gttctactac
      661 ggggatctta aatctcctgg atcagggtat tattgacgga cttgtgacct atgaccagtt
      721 cagccaggga tatttaagca taaaaaaagc agtggaagcc attcacggaa gcaggcagaa
      781 acaggaaact ttgttggatc cggtctatct ggatcagcag gaactggctt ccggcgcata
      841 tgagaaaatg ctctatccta tggagtaggg gggaaaagac gatgaagaga acaaaaggaa
      901 ccgggataat gctttttggt gtgcttgcga tcgtggtgat catcggagtg tttatcctgg
      961 caggaaacgg agctaaaaaa gaagctgcat ccaaggaaat acggataggg cttcttcttt
     1021 acagggggga tgataccttt atcagcaccc tgcgttctta tatcgaagaa aaggcgaaag
     1081 aatatgaacg gacagagggg atcaaggtga cactggatat tttggatgca aaaagcaacc
     1141 agaataccca gaacaaccag gtggaccggc tgctggagtt aggatgcaat gccttgtgtg
     1201 tgaatatcgt agatcgcttt gcagcttctg ttgtcattga taaagccatg gcagcagatg
     1261 tgcctgtgat cttttttaac agggaaccag tggaagagga tatgaaccgg tgggagaagc
     1321 tttattatgt aggggcagac gcaaaagagt ctgctgttct ggaaggaaag atcctggtag
     1381 atgcctacag gaaggatcct gcaagcctgg accggaacgg ggatggaacc gtcagctatg
     1441 tacttttaga aggggaaagc aatcaccagg actccctgat ccgtacagaa tggtctatcc
     1501 agacattaaa agacggggga gttcctctgg aaaaaatcac aggcggcatt gccagctggg
     1561 aacgcagtca ggcatctgcc atgatggaac agtggcttac cgactatccg gaccagatcg
     1621 aactggtcat ctgcaataat gatgatatgg ccttaggcgc gatcgatgcc attgagcggg
     1681 cggggatcct tcccggaacg atcaaattag caggtatcga tgcaacgcct cccggtgtgg
     1741 aagcgctgaa ggcaggaaag ctttttggaa ctgtagaagc ggataaagaa ggctatgcaa
     1801 acgctatttt cgggatcgca gcctctttaa gccggggact tcccattgat gagagcataa
     1861 aactggaaaa cggaaaatat ttctggtgca gccagaaagg actgacccag gctgatattg
     1921 caggaactga caataaatga cataaaaata ataatttttt tcagcaataa tgtaaaatca
     1981 aagaaatttc ataatactcc ttaaaaaatc ttatagaaaa atgcacaacg cggatttata
     2041 atgtgcttgt cgttaaggaa aaaacaccaa aagcgataaa aaaacaaaat catatgtata
     2101 gggaggatta caattatgag attagttaag aaagttacag cagcagctat ggcatccgca
     2161 atggcacttt cactggcagc ctgcggcggc ggaaacaccg ctacaacagc agctcctact
     2221 gaagcagcta aggaaactgc agcagcaagc gaagcagcta cagaggcagc taaggaagca
     2281 gcagagactg cagcaaatgc agctggaaca gcagatgttg ctgataagaa agtgggcatc
     2341 agcatttata aattcgatga taactttatg actctgtaca gacaggaact gcagcgttat
     2401 ctgaccgaag atttaggatt taagccagag aacgtagtta tccaggatgg taagggcgat
     2461 caggcagagc agaccaacca gatcaacaac ttcattaccc agaagtatga tgttctgatc
     2521 ctgaacctgg tacaggcttc ttccgcaccg gagatcacag atatgtgtaa agaagcagga
     2581 attcctgttg tttacatcaa ccgtgagcca gatgaggcag aagagcagag atgggttgac
     2641 gaaaagatca acgctactta tgtaggctgt gatgcaagac agtccggtac ctatcagggc
     2701 gaagagatct tagagacaga gaccaagggc gatatcaatg gtgatggaaa agtttcctac
     2761 atcatgatcc agggcgaccc tgagaacgta gatgcccagt acagaactga attctccgtt
     2821 aaggcattaa cagacgctgg cgtagaagta gaagaactgt taaagcagcg tggtgactgg
     2881 gatcaggcta aggctcagca gatcgcacag gatgctttaa accagtacgg cgacaagatc
     2941 gaagttatct tctgcaacaa cgacgcaatg gcactgggcg ctttacaggc aatcgaagca
     3001 gctggacgta aggtaaatga agatatttac ttagtaggtg tagatgctct gaccgaggca
     3061 gttcagaacg ttatcgatgg caagcagacc ggtactgtat ttaacgatca cttctcacag
     3121 gctaaggcag cagcagacgc agctgttcag ttcatcagcg gcgaagaagt tgatgcaaag
     3181 atccctgtag actacatcaa ggttactaac gcaaacgcac aggaaatcct tgacaagtta
     3241 aaataagtta cagttaactg actatttatc gggtgtgcgg gtcattccgt cacacccgat
     3301 ttttaaggaa aatatgctcc cggagggcat agaaaaagcg tcacaggaat tgaaaggagt
     3361 aacgggatgg cagagcagta cagactggaa atgataggag tcagtaaaag cttccctggc
     3421 gtaaaggcac tggatgggat aaacttgaaa gtacgtcccg gaactgtcca tgcgctgatg
     3481 ggggaaaacg gcgccggaaa atccaccctt atgaaatgtc ttttcggcat ttataagatg
     3541 gatgaaggac agatcattat tgatggtgaa aaaacggaga taacaaatcc ggatgatgcc
     3601 cttcgcaagg gcctggcaat ggttcatcag gaacttcagc caataccgga tcgttctatt
     3661 gcagaaaata tgtatctggg aagatatccc atgaagagtg ttggtcctct taaaatggtg
     3721 gatcacaaga ccatgaacag tgaagctgag aaatggttaa aagatgtaaa aatgaatttt
     3781 gatcctaagg caaaactggg aacattgtca attggacaga tgcagtcagt agaga
//
LOCUS       DBMDUA010000234         3817 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8553_length_3817_cov_1.842034, whole genome shotgun sequence.
ACCESSION   DBMDUA010000234 DBMDUA010000000
VERSION     DBMDUA010000234.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8553_length_3817_cov_1.842034"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(143..808)
                     /locus_tag="ACZPCL_12565"
     CDS             complement(143..808)
                     /locus_tag="ACZPCL_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HIR1163335.1"
                     /translation="MQDGIVIKSSKAGMTVILNPDLPFPDLLASIAKKFGDSARFWGN
                     AQMTLTLEGRPLSAEEEFQIVNTITENSQLQILCLLDTDAERIERCEKALNEKLMELS
                     AQTGQFYRGTLKRGDTLESEASIVIIGDVEHGARVTAKGNVIILGELKGTVTAGAAGN
                     DQAVILAFSMSPLQLRIGNHTSRFNEKNHKLGKGTMIASVENGELKVLPLKKPFFSSL
                     LDL"
     gene            complement(827..3760)
                     /locus_tag="ACZPCL_12570"
     CDS             complement(827..3760)
                     /locus_tag="ACZPCL_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1163336.1"
                     /translation="MFNELLEVTREFLKKVITSRLFALAVIFTLMFIGLICKLFRMQI
                     LDGSSYQESYMQRTEKLVTTPGTRGNIYDRNGNLLAYNQLAYAVAIQDLGDYPKAADR
                     NAMIYRLVTILERRGEKIDGKLEIALDENGQPYFTSTSNAAKKRFLLNFYGISSQQLD
                     DDTGKYPSNITAREAFELKKGSSRQGYKLADMKDADGNPMELSDQTALDMINIIYTME
                     LTRFQKYESTTVATNISQETMTEINENAADLKGVSIEPSSIRVYNDSLYFAPIIGYTG
                     KVQEDQIDSLNEEWNSSKEGQKSDAVNGVDKYDLNDIVGRIGIEKSMELDLQGEKGYT
                     RMYVDNMGRPREIIEQQDAQAGNDVYLTIDRDLQIATYNLIEQQLAGIITKFLVNEDI
                     DPATVRDGSKKPIPVKNAYYQLINNNVLSLDAMAGENASDIEKQIYRTYTASRDQILT
                     AIRGELLSDHAAAMNDLPKDMASYMNYIYSFLSSDNSGIVQRDKIDQNSQEYQAWKAG
                     TISLRDYIYSGIAGNWVDTTRLAASSKYSNADDIYSQLVDYIIAQLQDDKNFTKRMFR
                     YLVNDNVITGSQLCLALFAQGVLRDDPQARAGLATGDSAYTYNFIKNKIADLELTPAQ
                     LALDPCTAGCVVTDVKTGEIRALVSYPSYDNNRMSGSVDAAYFAKLQSDMSNPLYNNA
                     TQAIKAPGSTFKPITAIAALEEGVISLSDTIDCTGVYDQANPPINCWIYPGRHGTEDI
                     IAGIQNSCNVVFAELGHRLSMTADGTYSPEKGLSTIRKYASMFGLDHTSGVELPETEP
                     HLTTEDPERSAMGQGTNSYNNVQLSRYIAAVANRGTVFELSLLDKLTDSDGNLITDYT
                     PSVSSHVDAADSTWDAVQAGIRRVVTDGSAKSVFAGSPVEVAGKTGTAQEDRSRANHA
                     FFVSFAPYSNPEIAVTVNITYGYAGTNAATLGKKVYEYYYGYTSLEQVLGSGASSVSN
                     VTVGD"
BASE COUNT          959 a          898 c          816 g         1144 t
ORIGIN      
        1 ccccgccttt tccagaagta atcacaatga cttcactcat aggaacctcc tgaaaaattg
       61 agtttatatt actttaccaa tttattgtac attaaattgc gcaaatttac cagtattttt
      121 ttgagttcaa ccaaaaattc acctataaat ccagtaggct gctgaaaaat ggtttcttta
      181 aaggaagaac ttttaattct ccattctcta cagaagcgat catggttcct tttcccagtt
      241 tgtggttctt ttcattaaac cggctggtgt gatttccgat gcgcagctgc aggggagaca
      301 tggaaaaggc aaggatcact gcctggtcat ttcccgctgc accggctgta acggttccct
      361 ttagttctcc taagatgatc acatttccct tggctgttac cctggcgcca tgttccacat
      421 caccgatgat cacaatgctg gcttctgact ctaaggtatc tcctcttttt aaggttcccc
      481 ggtaaaactg gccggtctgt gcacttaatt ccattaattt ttcatttagt gctttttcac
      541 agcgttcgat ccgctctgca tctgtatcta acaggcagag gatctgaagc tgtgagttct
      601 ctgtaattgt atttacgatc tgaaattctt cttcggcgga cagaggcctt ccttccagag
      661 tcagtgtcat ctgggcattt ccccagaaac gggcactgtc tccaaacttt tttgcaatac
      721 tggcaagaag gtccggaaac ggcaggtcgg gatttaggat caccgtcata cccgccttgc
      781 tgcttttaat aacgattcca tcctgcatag aaacattcac ctctttttaa tctccaacag
      841 ttacatttga tacactggaa gcaccggagc ctaaaacctg ctccagactt gtatatccgt
      901 agtaatattc atagactttt tttccaaggg tagctgcgtt ggtacctgcg tatccataag
      961 taatatttac agtcacagcg atctccggat tgctgtaagg tgcaaaggat acgaagaatg
     1021 catggtttgc gcggctgcgg tcctcctgtg cagtaccggt ctttccggct acttctacag
     1081 gacttcctgc aaatacagat tttgcggaac cgtcggtaac aacccgccgg atacctgcct
     1141 gtactgcatc ccaggtactg tctgccgcat ccacatggga gctgacagaa ggagtataat
     1201 ctgtgatcag atttccatct gaatcagtca gtttatctaa aagacttaac tcaaatacag
     1261 ttcccctgtt tgcaacagca gcgatataac gggaaagctg tacattgtta taggagttgg
     1321 taccctgtcc cattgcagaa cgttccgggt cctctgtagt aaggtgtggt tctgtttccg
     1381 gcagttctac accggaggta tggtccaagc caaacatgct ggcatattta cggatcgtgg
     1441 aaagtccctt ttccggtgaa taggttccgt cagctgtcat acttaagcgg tgtccaagtt
     1501 ctgcaaatac tacgttacag gagttctgga tacctgcgat gatatcttcg gtgccatgtc
     1561 tgcctggata gatccagcag ttgatcggcg gatttgcctg gtcatataca ccggtacagt
     1621 caatagtatc agaaagggag atcactcctt cttccagtgc agcaatagct gtgatcggtt
     1681 tgaaagtaga tcccggagcc ttgatagcct gggtagcgtt gttgtaaagc ggattggaca
     1741 tatcgctttg cagttttgca aagtaagctg catctacgct tccggacata cggttattat
     1801 cataacttgg ataagaaacc agggcacgga tttctcctgt cttaacatct gttacaacac
     1861 agcctgctgt acagggatca agcgccagct gggccggtgt cagctccaaa tccgcgatct
     1921 tatttttgat aaaattataa gtgtaagcac tgtctcctgt tgcaagtccg gcacgggcct
     1981 gtggatcatc ccgtaaaacg ccctgtgcaa ataatgcaag gcacagctgg cttcctgtga
     2041 tcacattatc attgaccagg tagcggaaca tacgcttggt aaagttttta tcatcctgaa
     2101 gctgggcaat gatataatct actaactggc tgtaaatatc atctgcattg gaatatttac
     2161 tggaagctgc cagcctggtg gtatctaccc agtttcctgc aataccgctg taaatatagt
     2221 ctctcaggct gatggtgcca gctttccagg cctgatactc ctgggaattc tggtcgatct
     2281 tatctctctg gacaatgccg gagttatctg aggaaaggaa actgtaaata tagttcatat
     2341 agcttgccat atcctttgga agatcattca tggcagccgc atggtcactt aaaagttctc
     2401 cccttatagc agttaagatc tgatccctgg aagctgtgta ggtgcggtag atctgctttt
     2461 caatatcgga agcattctct cctgccatgg catccaaaga aagcacgttg ttattgatca
     2521 gctgataata agcattcttt acaggaattg gctttttgga accatctctt acagtggccg
     2581 gatcaatatc ttcgtttaca aggaatttgg taatgatacc ggctaactgc tgctcgatca
     2641 gattataggt agcgatctgc agatccctgt caatggtcag gtaaacgtca tttcctgcct
     2701 gtgcatcctg ctgctcaatg atctctctcg ggcgtcccat gttatctaca tacatacggg
     2761 tatagccttt ttcaccctga agatctaatt ccatggattt ttcaatgcca atacgtccta
     2821 caatatcatt aaggtcatac ttgtcgaccc cattgactgc atcgctcttc tgtccttcct
     2881 tggaactgtt ccactcttcg tttaagctgt cgatctggtc ttcctggact tttcctgtat
     2941 aaccgatgat cggagcaaaa tacagactgt cattgtatac acggatagat gacggttcaa
     3001 tggaaacacc ctttaaatca gccgcatttt cattgatctc cgtcatagtc tcctggctga
     3061 tattggtggc aacggtggta ctctcgtatt tctggaaccg ggtcagctcc atggtataga
     3121 tgatattgat catatcaagg gccgtctggt cagaaagttc cattggattt ccgtccgcat
     3181 ctttcatatc tgccagttta tagccctgac gggaggaacc cttctttaat tcaaaggctt
     3241 cccttgcagt aatgttggaa gggtattttc ctgtatcatc atctaactgc tgagaagaaa
     3301 taccgtaaaa attcagaaga aaacgttttt tagcagcatt ggaagtactg gtaaaatagg
     3361 gctgtccatt ttcatctaag gcgatctcca gctttccgtc gatcttttcc ccacggcgtt
     3421 ccaggatcgt cacaagacgg tagatcatgg cattgcggtc agctgccttt ggataatctc
     3481 ccaggtcctg aatggcaact gcataagcca gctgattata agccagcaga tttccattac
     3541 ggtcataaat attacctctg gtaccgggag tggtcaccag tttttctgtt cgctgcatat
     3601 aactttcctg ataggaactg ccgtctaaga tctgcatccg gaacagttta cagatcagac
     3661 ctataaacat aagagtaaat ataacagcca gggcaaagag gcgtgaagta atcacttttt
     3721 taagaaattc cctggtcact tccagcaatt cattaaacaa tggtcgtatc tctccttttt
     3781 ccaatatctt ccagtcttct gctggctgaa aggaaca
//
LOCUS       DBMDUA010000235         3809 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8573_length_3809_cov_2.541262, whole genome shotgun sequence.
ACCESSION   DBMDUA010000235 DBMDUA010000000
VERSION     DBMDUA010000235.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8573_length_3809_cov_2.541262"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="ACZPCL_12575"
     CDS             <1..634
                     /locus_tag="ACZPCL_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1163337.1"
                     /translation="GQNTFNSVFTTIMGTVTSLFFCALAGFAFAKYKFPGKTVLFYFV
                     IATMLVPPEVGSVPLFIIMKKVGLINSLWSLVIPRIATAVGIFYMNQYITDVPDELVE
                     AARIDGCTDFGIFMKIILPVIKPAMASWGAITLIARWNDFFWPLLYLRKQAKYTLMVT
                     ISLLPVSEGLSTPWPVILAGTTLVIIPIIILYLILERFQKAGMMEGAVKG"
     gene            826..3159
                     /gene="pcrA"
                     /locus_tag="ACZPCL_12580"
     CDS             826..3159
                     /gene="pcrA"
                     /locus_tag="ACZPCL_12580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HIR1163338.1"
                     /translation="MSIYDTLNPKQKEAVLHTDGPLLILAGAGSGKTRVLTHRIAYLI
                     DECGVNPWNIMAITFTNKAAGEMRERVDDLVGFGAENIWVSTFHSSCVRILRRHIESL
                     GYTTSFSIYDSDDQKTLMRQVFKAMNVDTKQFKERSVLAAISSAKDKLITPEEFLLNA
                     GADFREKKTGEIYKEYQKQLRKNNALDFDDLIVKTVELFQNNPQVLDYYQERFKYIMV
                     DEYQDTNMAQFKLVSLLASKYRNLCVVGDDDQSIYRFRGADIQNILSFEHTFPGTKVI
                     KLEQNYRSTQNILDAANGVIRHNFGRKDKTLWTANGEGDKILFKQFDTARDEADFVAR
                     QIRDSSYSYQDQAVLYRTNAQSRLIEERCIFYNIPYRLVGGVNFYQRKEIKDVLAYLK
                     TIANGVDDLSVIRIINVPKRGIGATTIGRVTAFASEHGMSFYDTLKEAKQIPGIGKAA
                     EKISRFIAQMEAFRAMAHSEEYSIKDLIDHILEDTGYEEELQEEGEIEAQTRLENIEE
                     LINKAAAYEEDSEHPTLDEFLEQVALVADIDNVDDSEDRVTLMTLHSAKGLEFPKVYL
                     VGMEDGLFPGMMSIMSGDKTEMEEERRLCYVGITRAKKELVLTAARQRMVNGETRWSK
                     PSRFINEIPPELLDTDKLQPVLGKGKQDDFDESGLPWNQGTKSGVSRFGMGYNAYASK
                     TASPTGSSKSSGMGGFGSSMQSLEPKKKPGFGKTFTVQKAASLDYKEGDRVKHAKFGE
                     GTVKEIIDGARDYEITVEFDKGGQKKMLAGFAKLERV"
     gene            3235..3576
                     /locus_tag="ACZPCL_12585"
     CDS             3235..3576
                     /locus_tag="ACZPCL_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HIR1163339.1"
                     /translation="MNRFDRERWDTMGKEALSRVEELMNTSRINELIHKREEDEKKKN
                     CILWVLAIIGAVAAVAGIAYAVYRFFTPDYLEDFEDDFDDDFDDDFFEDEKAEEKKSE
                     KKEERAEEKAE"
BASE COUNT         1206 a          701 c          961 g          941 t
ORIGIN      
        1 aggccagaat acatttaaca gtgtatttac caccattatg ggtactgtaa cttccctgtt
       61 tttctgtgca ctggcaggtt ttgcttttgc aaagtataaa ttcccgggaa agacagtttt
      121 attctatttt gtaattgcta ccatgctggt accgccggaa gttggttctg taccactgtt
      181 catcatcatg aaaaaagtgg gactgatcaa cagcctctgg tctttagtta tcccaagaat
      241 tgcaacagca gttggtatct tctacatgaa ccagtatatc accgatgttc cggatgaact
      301 ggtagaagca gccagaattg acggctgtac ggattttggt atctttatga agatcatcct
      361 tccggttatc aagccggcta tggcttcctg gggtgcgatc acactgatcg caagatggaa
      421 tgacttcttc tggccattac tttacttaag aaaacaggca aaatacacat tgatggtaac
      481 catttcctta ctgccagtca gtgaaggttt gtctacacca tggcctgtta tccttgcagg
      541 taccacatta gttatcattc ctatcattat actgtatctg atcctggaac gtttccagaa
      601 agcaggtatg atggaaggtg ctgtaaaggg ctaaataatg aaaatgaccg atctttatta
      661 aagggttcgg agaaaataag aacagaatgc cgtgtttcag gctgtatgga gaacgcctgg
      721 aacatggcat ttttgtgttt agggagaaca tttgtttttg tgcctgcttg caggataggt
      781 acagggatgt tataatgact ccagagaaaa aaaggcggta ataatatgag catttatgat
      841 acattaaatc caaaacaaaa agaggcagtc ttacatacag acggccctct tcttatactg
      901 gcaggcgccg gttccggaaa gacaagggtc ctgacccaca ggatcgctta tctgatcgat
      961 gaatgtgggg taaatccatg gaacatcatg gcgatcactt ttaccaataa agcagccggg
     1021 gaaatgcggg aacgtgtgga tgacctggtt ggctttgggg cagagaatat atgggtcagc
     1081 accttccatt cttcctgtgt gcgcatcctg cgccgtcata ttgaaagtct gggctatacc
     1141 accagttttt ccatttatga cagtgatgac cagaagaccc tgatgcgtca ggtgtttaag
     1201 gcaatgaatg ttgataccaa acagtttaaa gaacggtctg tcctggcagc tatttcttct
     1261 gcaaaggaca agctgatcac tccggaagag tttctgttaa atgcaggagc ggatttcagg
     1321 gaaaagaaga caggggagat ctataaagaa taccagaagc agctgcgcaa aaataatgcc
     1381 ctggactttg atgacctgat cgttaagacc gtagaactgt tccagaataa cccgcaggta
     1441 ctggactatt accaggaacg ttttaaatat atcatggtag acgagtacca ggataccaat
     1501 atggcccagt ttaagctggt aagcctcctg gcttccaagt accgcaatct ctgcgtagtg
     1561 ggtgatgatg accagtctat ttaccggttc agaggtgcgg atatccagaa tatcttaagt
     1621 tttgaacata cattcccagg cacaaaggtg atcaaactgg agcagaatta ccgttctacc
     1681 cagaatatcc tggatgcggc aaatggagtg atccgtcata acttcgggcg aaaggataag
     1741 actctgtgga cagccaacgg tgaaggagac aagatcttat ttaagcagtt tgatacagca
     1801 agggatgagg cggattttgt agcccgccag atccgtgaca gttcatattc ctatcaggat
     1861 caggcagtgc tttacaggac caatgcacag tcacgactga tagaagaacg gtgtattttc
     1921 tataatatcc cttacagact ggtgggagga gtcaacttct atcagagaaa agaaatcaag
     1981 gatgtactgg cgtatttaaa gactattgca aatggagtgg atgacctttc cgtgatccgt
     2041 atcatcaatg tgccgaaacg ggggatcgga gctaccacca ttggaagagt aactgctttt
     2101 gcgtcagaac acggaatgag cttttatgat actttaaaag aggcaaaaca gatcccgggt
     2161 atcggtaagg cggcagaaaa gatcagccgt tttattgccc agatggaagc cttccgggcg
     2221 atggcacatt cagaagagta ctctattaaa gatctgatcg atcatattct ggaagatacg
     2281 ggttatgaag aggaacttca ggaagagggc gagatcgagg cccagacccg tctggaaaat
     2341 attgaggaat tgatcaacaa agcagctgcc tacgaagaag acagtgaaca tcctacactg
     2401 gatgaatttt tggagcaggt ggcgctggtt gcggacattg acaatgtaga tgactcggaa
     2461 gacagggtaa ccttaatgac ccttcacagt gccaagggcc tggaatttcc aaaggtctat
     2521 ttagtaggta tggaggacgg gcttttcccg ggaatgatgt ccattatgtc tggtgataag
     2581 acagaaatgg aagaagagcg caggctttgt tatgtaggta tcacccgtgc aaagaaagaa
     2641 ctggtattaa ctgcagccag acagcgcatg gtaaacggag agaccagatg gtccaagcca
     2701 agccgcttta ttaatgagat cccgccggaa cttttagata cggataaatt gcagccggtc
     2761 cttggaaaag gaaagcagga tgactttgac gagtcaggac ttccatggaa ccagggcact
     2821 aaaagcggtg tcagccggtt tggaatggga tataatgcct atgcttctaa aacagcatca
     2881 ccaacgggaa gcagtaaaag ttctggaatg ggcggttttg gcagcagcat gcaaagctta
     2941 gagccaaaga aaaagccggg ctttggaaag acatttacag tccagaaagc agcatccctt
     3001 gactacaaag aaggggaccg tgtaaaacat gcaaaatttg gcgaaggaac tgtaaaagag
     3061 atcatagacg gggcaaggga ttatgaaatc acggtagagt ttgacaaagg tggccagaag
     3121 aagatgttag cgggatttgc aaagctggaa cgtgtctgaa aacagtagta aaaagtaaga
     3181 gtttatggta tactaaataa caaggaaaat aagtgcagaa aggatggacc tgctatgaac
     3241 agatttgaca gagaaagatg ggataccatg ggaaaagagg ctttaagccg tgtagaagaa
     3301 ctgatgaata ccagccgtat caacgaactg atccacaaga gagaagaaga tgaaaagaaa
     3361 aagaactgta ttctctgggt actggctatt atcggtgcag tagcagcagt agcaggtatt
     3421 gcatacgcag tttaccgttt ctttacccct gattatctgg aagattttga agacgatttc
     3481 gatgatgact tcgacgatga cttcttcgag gatgaaaagg cagaagagaa gaaaagcgaa
     3541 aagaaagaag agagggctga ggaaaaagct gaataaaata agaaagaagc cataacaggt
     3601 gtaaaatctt ataatttaaa aagcgtgtct catataaaag ggacacgctt ttcttaggta
     3661 taggttgcta ttcgggcata tttgtgataa ataagtgata gactgaaata tttaaagtga
     3721 cagaatacaa aataaggagt agaaaagtga aaaaaggcga gatttacaaa gcgattgtag
     3781 aaaaggtaga attcccaaac aaaggtatt
//
LOCUS       DBMDUA010000236         3761 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8687_length_3761_cov_2.674590, whole genome shotgun sequence.
ACCESSION   DBMDUA010000236 DBMDUA010000000
VERSION     DBMDUA010000236.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8687_length_3761_cov_2.674590"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="ACZPCL_12590"
     CDS             <1..301
                     /locus_tag="ACZPCL_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:dicarboxylate symporter family
                     transporter"
                     /protein_id="HIR1163340.1"
                     /translation="FHMEYATPRNILMSILVAVIASVVMGGIPAGGYVAEIFIISAFG
                     FPTVAIPIMVLIGTITDAPATVVNVTGDTGLAMVITRFVEGKDWIHRKKLAQKAA"
     gene            449..1765
                     /locus_tag="ACZPCL_12595"
     CDS             449..1765
                     /locus_tag="ACZPCL_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HIR1163341.1"
                     /translation="MENKKLGALAFLPLIVFLGTYIGCGIVFSVMGAESPFGYFPRHV
                     ALLVGIASALILAPGVKISERVDVLTENMGRSGVMMIVLIYLMASGFQGAAAAIGGKT
                     SVINLCLHYIPVKLLVPGVFMMCCFISTAIGTSMGTMAAMAPVALGVAEGAGLNAAIV
                     CAAVIGGSYFGDNLSMISDTTIAAAEGCGSEMRDKFRMNFFIAFPAAVLSLIFYGFIG
                     GAGSGSIEVGAFDLFQIAPYIVVLVTALMGINVAVVLFIGILMTFIVGLLQGNVGFFD
                     IAMAVGDGMADMFSISIVAILVSGVIGLVRYYGGIEWMIDTITKKIKNRKQAEYGIGI
                     LSGILSGAMVNNTIAIIVTCPIAKEIGSKYKIAPKRLASLVDIFACAFLAVMPHDGGM
                     LMTSEMANISPFAILPYSVYPLFLIIATCITIQAGLLRTAEEKQKK"
     gene            1984..2472
                     /locus_tag="ACZPCL_12600"
     CDS             1984..2472
                     /locus_tag="ACZPCL_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1163342.1"
                     /translation="MFQGIKKLFGGKKEEGQILAPVAGQAVPLSEVPDPAFAQEILGK
                     GVAILPSENEFKAPASGEVTVLFETGHAVSIRTESGADVIVHIGLDTVNLKGQFFITH
                     VKQGDTVKAGDLLITADIEQIKAAGYEVITPVIVCNVSDFPDMVCHTGKAVKAMDEII
                     TL"
     gene            2575..>3761
                     /locus_tag="ACZPCL_12605"
     CDS             2575..>3761
                     /locus_tag="ACZPCL_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR1163343.1"
                     /translation="MMKFFQKLGKSLMLPVACLPVCGILMGIGYILAPAAMAGEVQGF
                     TAGGLSYAIGLFLIKAGGALIDNMSWLFAVGVGVGMADDSDGTAGLAGLVSMYMITTL
                     LSPGAVAGITGHEAAPAFGKIINQFVGILSGLIGSACYNKYKTVKLPDALAFFSGKRA
                     VAIFTAIYSIVAALVLFVVWPLVYGGLVALGEAFIGMGAVGAGIYAFFNRLLIPFGLH
                     HALNSVFWFDVAGISDLSKFWGNTGVYGQTGMYMTGFFPFMMFGLPAACIAMYQTAKP
                     AKKKMVYGLLASAAFCSFFTGVTEPIEFSFMFLAPGLYVVHALLAGITAGITVALPIR
                     AGFSFSGGAVDMVLSSFTPLAENPWLLIPVGIAVGVLYYIVFRFAIAKFNLKTPGRED
                     DDA"
BASE COUNT          966 a          740 c          949 g         1106 t
ORIGIN      
        1 attccatatg gaatatgcaa ccccacgcaa cattttaatg tctatcctgg tagctgttat
       61 cgcatctgtt gttatgggag gaatccctgc aggcggttat gtagctgaga tctttatcat
      121 atctgcattc ggcttcccta cagtagctat cccgatcatg gttctcatcg gaaccattac
      181 cgatgcacct gcaactgtag tcaatgtaac aggtgatact ggacttgcca tggtcattac
      241 aagatttgta gaaggaaaag actggatcca cagaaaaaaa ctggcacaaa aagcagcata
      301 aaataataat ataactgcgc atccctcatg gtcggaccgg atctgacata tgggggtatt
      361 gtgcgtttct ttacaagcgt ttataatggt ttaatgggaa tacaaaaatg ctttgcagaa
      421 gatgcaggca cgaaaaggag aatttcttat ggaaaacaag aaactggggg cgttggcctt
      481 tctgccgctg atcgtatttt taggaactta cattggttgt ggtattgtgt tttcggttat
      541 gggagcggaa agtccttttg gctatttccc acgccatgta gcgttgcttg tgggaattgc
      601 gtctgccctt attctggcac cgggggtgaa gatttcagag agggtagatg tccttacaga
      661 aaatatgggc cgcagcggtg ttatgatgat cgttctgatc tatcttatgg ccagcggatt
      721 tcagggcgca gcagcagcta ttggcggcaa gacttctgta attaacctgt gtttacacta
      781 tattccggtc aagcttctgg ttcctggcgt atttatgatg tgctgcttta tctctacagc
      841 catcggaaca tccatgggta ccatggcagc tatggctccg gttgctttgg gcgtagcgga
      901 aggcgcaggc ttaaatgcgg ctatcgtctg tgcggcagtg atcggcggtt cttattttgg
      961 tgataacctg tccatgatct ctgataccac cattgcagca gcagaaggct gcggatctga
     1021 gatgcgtgat aagttccgca tgaacttctt cattgccttt ccggcagctg ttctttctct
     1081 gatcttttat ggatttatag ggggagcagg cagcggaagc attgaagtag gtgcttttga
     1141 cctgttccag atcgctcctt atattgtcgt tttagtaact gcgttaatgg gtatcaatgt
     1201 agctgtggta ctgttcattg gtattttaat gacctttatt gtaggactct tacagggaaa
     1261 tgtaggcttc tttgatatcg ccatggcagt aggtgatgga atggctgata tgttcagcat
     1321 cagtattgtt gctatcctgg tatccggtgt gatcggtctg gtacggtatt acggcggtat
     1381 tgaatggatg atcgatacga tcaccaaaaa gatcaaaaac agaaaacagg cagaatatgg
     1441 tatcggtatc ctttccggta ttttatccgg tgctatggtc aataatacga ttgctatcat
     1501 cgttacctgt cctattgcaa aagagatcgg cagcaagtac aagatcgcgc caaagcgtct
     1561 ggcaagcctg gtagatattt ttgcctgtgc attcttagct gttatgcctc atgacggcgg
     1621 tatgctgatg acatctgaaa tggcgaacat atccccgttt gctatcctgc catactctgt
     1681 ttacccatta ttcctgatta ttgcaacctg catcacgatc caggcaggac ttttaaggac
     1741 agcggaagaa aaacagaaaa aataggcaga aaaagcaata ataaaatagg atttttcggt
     1801 aaaatagaca aagaccgaaa gaaagtgcat tgacttatag gggtaatgat gatattatag
     1861 ccctatcgtt attttcttga taatattggt tgctctaagg caacacgcaa aaccaatttt
     1921 gaacgatatt atgtgaaaca gatatttgtt caaaattggt ttttgtttta agggggaatt
     1981 attatgtttc agggaattaa aaaattattc ggtggaaaaa aagaggaagg ccaaatcctt
     2041 gcacctgtag ccggtcaggc agtgccttta agcgaggtgc cggatcctgc atttgcccag
     2101 gagatactgg gaaaaggcgt agccatcctg ccttcggaaa atgaatttaa agcgcctgca
     2161 tccggagagg taactgtatt atttgaaacc ggacatgcgg tcagtatccg tacagagagc
     2221 ggtgcagatg tgatcgttca cattggctta gacactgtga atttaaaggg gcagttcttt
     2281 atcacccatg taaaacaggg tgacacagta aaggcagggg atctgctgat cacagcagat
     2341 atagagcaga tcaaagcagc ggggtatgag gtaataacac cggtgatcgt ctgcaatgtt
     2401 tctgatttcc cggatatggt atgccatacg ggaaaagccg taaaagccat ggacgagatc
     2461 attactctgt aatgaacatc aacatggata taaaggggat ttgaaaccag gagaagggtt
     2521 caaataaata agccgggata aaaaaacggc aacaaaagaa aggggttttt agttatgatg
     2581 aagttctttc agaaacttgg taagtcactg atgcttccag tggcgtgtct gccagtatgt
     2641 ggtatcctta tgggtatcgg atacatcctg gcaccggcag ctatggcagg tgaggttcag
     2701 ggatttaccg caggaggctt atcgtacgct atcggactgt ttctgatcaa agcgggggga
     2761 gcattgatcg ataatatgtc ctggctgttc gcggtaggtg taggcgtagg tatggcagat
     2821 gattcagatg gaacagcagg tctggcaggt cttgtatcta tgtatatgat caccacactg
     2881 ttaagtcccg gtgctgttgc cggtattacc ggacatgagg cagcgcctgc atttggtaag
     2941 atcatcaacc agttcgttgg tattctttcc ggtcttatcg gttctgcatg ctacaacaaa
     3001 tataagactg ttaaactgcc agatgcactg gcattcttca gcggcaagag agcagttgcg
     3061 atctttacag ctatttattc catagttgca gctttagtcc tgtttgtagt atggcctctg
     3121 gtttacggtg gtctggtagc attaggcgaa gcatttatcg gaatgggtgc tgttggagca
     3181 ggtatctatg cattctttaa ccggcttctg atcccatttg gcctgcacca tgcgttaaac
     3241 tctgtattct ggtttgatgt tgcaggaatc agtgacctga gcaaattctg gggcaatacc
     3301 ggtgtttacg gacagactgg tatgtatatg accggattct tcccatttat gatgtttggt
     3361 cttccagcag cctgcatcgc tatgtatcag acggcaaaac ctgctaagaa gaaaatggtt
     3421 tacggacttt tagcttctgc agctttctgt tctttcttta caggtgttac agaaccaatt
     3481 gagttttcct ttatgttcct ggctccaggg ctgtatgtag tgcatgccct gttagcaggt
     3541 attacagcag gcatcaccgt tgccctgccg atccgtgcag gcttctcctt ctcaggcggt
     3601 gctgttgata tggtactcag ttcctttacg ccacttgcag aaaatccatg gctgctgatc
     3661 ccggtaggta tcgcagttgg cgttctttat tatatcgtat tccgtttcgc tattgccaag
     3721 tttaacttaa agactccggg acgtgaagat gatgatgcag a
//
LOCUS       DBMDUA010000237         3756 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8699_length_3756_cov_2.466211, whole genome shotgun sequence.
ACCESSION   DBMDUA010000237 DBMDUA010000000
VERSION     DBMDUA010000237.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8699_length_3756_cov_2.466211"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(64..1611)
                     /locus_tag="ACZPCL_12610"
     CDS             complement(64..1611)
                     /locus_tag="ACZPCL_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR1163344.1"
                     /translation="MFTQISSAGIRGMEGFLVSVEADVSNGLPGFSISGQLALEVREA
                     QERVRTALKNSDFQLPAKKITVNLSPAGRRKEGTVFDLPIAAAVLGAFELLSAEKLKD
                     ALLIGELGLDGSVKPVRGVLVLVSAAKKMGFRRCLLPVANVSEGTLVEGIQIIGIQSL
                     RHLQEVLSQDNWEDMDLEISGNINRRTFKPEYLFDFKEICGQSVLRRAAEVAAAGRHG
                     LLMCGSAGTGKSMVAKRIPTILPALSWEENIEISKIYSLCGLLPKGQPLLSQRPFRCP
                     HHTISPQGLTGGGKVPKPGELSLASGGVLFLDELPHFSKGAIEALREPLEDHRITVSR
                     VAGSYEFPADFLILGAMNPCPCGFFPDRSRCSCTPMQIQNYLNRLSRPILERFDICVE
                     AAPVSFLELEDQTMANEDSATIRSRVEKAVKIQNYRFQGTDIHSNSRMGQKEIQRFCQ
                     LKEAERRFARKVYEARGISARGYHRVLKTARTIADLTGEEWIQKEHLSEAFGYRALEE
                     RIWGQKH"
     gene            complement(1845..2600)
                     /locus_tag="ACZPCL_12615"
     CDS             complement(1845..2600)
                     /locus_tag="ACZPCL_12615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HIR1163345.1"
                     /translation="MTIFLCEPGYTGILSGIYDAGISKIPQDELRLEIKNSGQNVELF
                     AVYRECSPSEEKGKKVLETVRQRLNSHIQEQLYVASLSEDVLRADKMYRYLKVLFLRG
                     ASAADQLQIPAVHEIFKLCRSVYNEKHQFIEFLRFSQMEGGFLAGKIQPKNDVLELIA
                     PYFADRLGEENWLICDIGRKKAVIHERGRKWLLAELSSDEMERLQREGTEENWVKLWK
                     TFFTAIAIEERKNPNCQRNHLPLRFRDLMTEFQ"
     gene            complement(2622..>3756)
                     /locus_tag="ACZPCL_12620"
     CDS             complement(2622..>3756)
                     /locus_tag="ACZPCL_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HIR1163346.1"
                     /translation="IYETLYKLRNIYCFNGYIHVKSIPGASRELVEKTGFLADRMSIN
                     LELPTAAGLRELAPGKTREKILAPMRQVQQGIALSGRLLGYDRGYRKMFPSDRAGFAG
                     GASLIQPELTGKDLLMPFGKADSPALALKERHYGTKAFVPAGQSTQMIVGATPENDFQ
                     MLSVTQALYHNFGLKRVFYSAYVPVNEDSHLPSLPGGPPLLREHRLYQADWLLRFYGF
                     KAEELLSEKKPNFNLLLDPKCDWALQNLKEFPVEVNKASYEQLLRVPGVGVKSARRII
                     AARKQGKLDFDGLKRIGVVLKRARYFITCSGKMEVSCSLDEDHITSALIGDERRKIWD
                     IENRNSYRQLSLFDDMHLEEEKNSLLKDQLVKEAAGSAAFGQL"
BASE COUNT          943 a          998 c          710 g         1105 t
ORIGIN      
        1 tccgacgtat ctgcaaagcc cctgcttccg gcagatgaca aagccagtac aaagatactt
       61 tttctaatgc ttctgccccc atatccgttc ctccaatgca cgatagccaa aggcttctga
      121 taaatgttct ttctgtatcc attcttctcc tgtcagatct gcaatggtcc tggctgtttt
      181 taataccctg tggtatcctc tggcacttat cccccttgcc tcatatactt ttcttgcaaa
      241 ccggcgctct gcttctttta actgacaaaa acgctggatc tccttctggc ccatgcggct
      301 gttgctgtgg atatccgttc cctggaagcg ataattttgt atcttcactg ccttttccac
      361 ccggctgcgg atcgtagcgg agtcttcatt tgccatggtc tggtcttcca attccaaaaa
      421 tgaaaccggc gctgcttcca cacagatatc aaagcgttct aagatcggtc ttgaaagccg
      481 gtttaaataa ttttgtatct gcatcggtgt acaactgcac ctgcttctgt ctggaaagaa
      541 cccgcaggga cagggattca tggctccaag gatcagaaag tctgccggga attcgtagct
      601 tcctgctaca cggcttactg taatacggtg atcttccaaa ggttctctta aagcctctat
      661 ggcccctttg ctgaaatgag gcagttcatc taaaaacagc acacctccgg aagcaaggga
      721 taattcccct ggtttaggca cttttccgcc gcctgtaagg ccctggggtg aaatcgtatg
      781 atgtggacag cggaaaggtc tttgggaaag caatggctgt cccttgggaa gtaatccgca
      841 aagactgtaa atctttgata tttctatatt ttcttcccag gaaagagctg gcaggatcgt
      901 aggaatacgc tttgccacca tggattttcc tgttcctgcg cttccgcaca tcaaaagccc
      961 atgtcttccg gctgctgcta cttctgcagc ccggcgcaaa acactctgac cacatatttc
     1021 tttaaaatca aatagatact ctggtttaaa agtccgtctg tttatattcc cagatatttc
     1081 cagatccata tcttcccagt tatcctgtga taaaacttcc tgtaaatgac gaagggactg
     1141 tatccctatg atctgtatcc cttctaccag agttccctct gatacattcg ccaccggtaa
     1201 aaggcaccgc ctaaatccca tcttttttgc agccgatacc aggaccagca caccccttac
     1261 aggtttgaca ctgccgtcta atcctagctc cccgatcagc agggcatctt ttagtttttc
     1321 tgctgataac agttcaaacg cccctaaaac agcggctgca ataggaagat caaagactgt
     1381 tccctccttt cgtcttccgg ccggtgacag atttaccgtt attttctttg ccggaagctg
     1441 aaaatctgaa tttttcagcg ccgtcctcac ccgttcctga gcctctctta cttccagtgc
     1501 cagctgcccg gaaatagaaa acccaggcag tccattggat acatctgctt ctacagatac
     1561 cagaaatcct tccattcccc ggatcccggc actgcttatc tgtgtaaaca tgcgcaacgc
     1621 tcccttcttt tgttttttcg acaaatggat acactttccc ctgcccttcc ctgtttattt
     1681 tgggattaaa atagaattag taaaagtaaa aataatagat actttagaat aattaaagat
     1741 accagacgta tatatcacct gagggtgaca tcaccagtgt atccaacaaa agaagaaatt
     1801 gcaagcctat ttttacgaac tgcaacaaat ctgcaacaaa tttatcactg aaactccgtc
     1861 ataagatccc tgaaccgtaa aggcaggtga tttctctggc agtttggatt ttttctctct
     1921 tcaatagcga tcgctgtaaa aaaagtcttc caaagtttga cccagttttc ctctgttcct
     1981 tccctttgca ggcgttccat ctcatcagat gaaagttcag ccaaaagcca ttttctccct
     2041 ctttcatgta ttactgcctt ttttcttcct atatcacaga tcagccagtt ttcttctccc
     2101 agccggtctg caaaataagg tgcgatcagt tctaacacat catttttcgg ctgtatcttt
     2161 ccggcaagaa agcctccctc catctgcgaa aaccgcaaaa attcaataaa ctgatgtttt
     2221 tcattataca cactgcgaca cagcttaaat atctcatgga ctgcagggat ctgcagctgg
     2281 tctgcggccg atgccccccg caaaaacagc actttcagat accggtacat tttatccgcc
     2341 cgcaaaacat cctcgctgag actggccaca tataactgct cctgaatatg ggaattcaat
     2401 cgctgacgta ctgtctccaa tacctttttc cctttttctt ctgatggact gcattccctg
     2461 tatacagcaa aaagctctac attttgccca ctgtttttta tctccagccg cagttcatcc
     2521 tgtggtatct tactgattcc cgcatcatag ataccactta agatccctgt ataaccaggt
     2581 tcgcacaaaa agatcgtcat gttcgtttct cctgtatttt ctcataactg tccaaaggca
     2641 gcacttcccg ctgcttcctt taccagctga tcctttaaaa gagagttttt ctcttcttcc
     2701 agatgcatat catcaaacag ggaaagctgc ctgtagctgt tcctgttttc aatgtcccat
     2761 atcttccgtc tttcatctcc tataagggca ctggttatat gatcttcatc caggctgcag
     2821 gagacttcca ttttcccgga acaggtgata aaataacggg ctctttttaa aaccacaccg
     2881 atccgtttta agccatcaaa atccagtttt ccctgttttc tggcagcaat gatccgtctg
     2941 gctgatttta cgcccactcc cggcacgcga agaagctgtt cataagaagc tttgtttact
     3001 tctaccggga actctttcag attttgcagc gcccagtcgc attttggatc cagtaacagg
     3061 ttaaaattcg gtttcttctc ggaaagaagt tcctctgcct taaatccgta aaaacgtaaa
     3121 agccagtcag cctgataaag cctgtgctcc ctcaaaaggg gcggtccccc cggaagagac
     3181 ggcagatggc tgtcttcatt aaccggaaca taggcagaat aaaaaacacg ctttagtcca
     3241 aaattgtgat aaagggcctg tgtcacggaa agcatctgaa aatcattttc cggtgtagcg
     3301 cctacgatca tctgtgtact ctgtcctgca ggcacaaagg cttttgtccc ataatggcgc
     3361 tcttttaatg ccagtgccgg actgtctgct ttgccaaaag gcataagaag atctttccct
     3421 gttaattcag gctgtatcag agatgctcct cctgcaaaac cagccctgtc agaagggaac
     3481 attttcctgt atcccctgtc ataccctaac agacgtccgg acaaagcaat cccctgctgt
     3541 acctgacgca taggtgcaag tatcttttcc cttgtttttc caggtgccag ttctcttaat
     3601 cctgcagctg taggcagttc cagattgata ctcatacggt ctgccagaaa tccggttttc
     3661 tctaccagtt ccctggaagc tccgggaatg gattttacgt ggatatatcc attaaaacag
     3721 taaatattgc gcagcttata aagggtctca tagatc
//
LOCUS       DBMDUA010000238         3743 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8734_length_3743_cov_1.966227, whole genome shotgun sequence.
ACCESSION   DBMDUA010000238 DBMDUA010000000
VERSION     DBMDUA010000238.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8734_length_3743_cov_1.966227"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="ACZPCL_12625"
     CDS             <1..661
                     /locus_tag="ACZPCL_12625"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1163347.1"
                     /translation="FDLDGTLVNSIYALKRSVDLTLGHFGLGPISIEDTKQFVGDGYK
                     KLVERSLMAYGDKELTHYKEALDVYNEVFKDCCLYKVEAYEGIPQLLDFLKEHGILAT
                     VLSNKPHQRALENVAHVFGDHTFDKVYGEREALGIRKKPAPDGVVALIEELGLEKEDC
                     LYLGDTNTDMETGQAAQVDTVGVTWGFRPREELEAFKPKLVAESPFQVIDFIKAVNHI
                     E"
     gene            744..1814
                     /locus_tag="ACZPCL_12630"
     CDS             744..1814
                     /locus_tag="ACZPCL_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR1163348.1"
                     /translation="MCCILLSGCGISGTVGQNSSKVSDEKDTRPLVVTTIFPYYDFVR
                     QIAGDRVRLKMVVPAGMDSHSFEPTPADMITMQQADLMVCNGGEMEQWVEKVVASLDT
                     ENMKVLTMMDYVDVLEEEIVEGMEDNEHSHDDHGHAHENEEEDHDHSEMDHSRPDDEI
                     KTHENLSQITFDDHDGHEMDIEYDEHIWTSPANAMKLVEVITETLKEVSPENASYFEE
                     NKNSYETELKSLDDEFKDIVANGRHRMIVVADKFPFRYFAQEYGLSYRAAFSGCSTDT
                     EPSAKTIAYLIDRIKENQIQAVFYLELSSHRTADIISEETGAKPLLLHSCHNVTRRQF
                     DEGVTYLQLMKQNAKNLQEALL"
     gene            1830..2681
                     /locus_tag="ACZPCL_12635"
     CDS             1830..2681
                     /locus_tag="ACZPCL_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIR1163349.1"
                     /translation="MEPLIKCEHVDLGYENQDAVINVNMEVCPGDYLCIVGENGSGKS
                     TLIKGLLGLLKPSGGKLTVAEELKTTGIGYLPQQTAAQKDFPASVREVVLSGCLSRRG
                     RRPFYSKTEKDIASANMEKLGITQLANQCYRELSGGQQQRVLIARALCATRELLILDE
                     PITGLDPMAIQDFYSMIRKLNREDKVAIIMVSHDLRNAVEEANKILHLQKQVLFYGPA
                     HDYMNSKAAGHFFHEKEQGECLPTAACHMAKNVDGLENNEQKCSCGHTHAHSPQKIEG
                     GNTHERA"
     gene            2668..3486
                     /locus_tag="ACZPCL_12640"
     CDS             2668..3486
                     /locus_tag="ACZPCL_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR1163350.1"
                     /translation="MNVLSEMLSYPFMVRALFGGMLVSLCASLLGVSLVLKRYSMIGD
                     GLSHVSFGALSIALAMGWSPLKVSIPVVVLAAFFLLRITENSRIKSDAAIAMISASSL
                     AIGIIVTSLTTGMTTDVSSYMFGSILAMTKEDVVLSAVLCIIVLGLFVLCYNQVFAVT
                     FDENFAKATGVNVGAYNMLISVLTAVTIVLGMRMMGAMLISSLVIFPCLTSMRVFKSF
                     TSVVISSGILSLICFLLGMMASYQFSTPAGASVVVVNLIAFGLFSMWQVLGRKR"
BASE COUNT         1130 a          741 c          895 g          977 t
ORIGIN      
        1 atttgatctg gacggaacat tggtgaactc tatttatgct ttgaaacgtt ctgtagacct
       61 gacactgggc cattttggac tggggccgat ctctattgaa gataccaagc agtttgtagg
      121 agatggctat aaaaagctgg tagaacgttc cctgatggca tatggggata aagaactgac
      181 tcattataag gaagcgttgg atgtatataa tgaagttttt aaggactgtt gtctttacaa
      241 agtagaagca tatgagggaa ttccacagct tctggatttc ttaaaagaac atggcatact
      301 ggcaaccgta ctgtccaata aacctcatca gagagccctt gagaatgtag cccatgtatt
      361 tggagatcat acatttgaca aggtatacgg agagagagaa gcattaggca tccgcaagaa
      421 accggctccc gacggtgtag tggctctgat cgaagaactg gggcttgaaa aagaagactg
      481 tttgtatctg ggagatacta atacagatat ggaaacagga caggcagccc aggtagatac
      541 agtaggtgtg acctggggct ttcgtccgag agaagagctg gaggcattta agccaaagct
      601 tgtagcagag tctcctttcc aggtgataga ttttataaag gcagtgaacc acattgaata
      661 aatttgaagt gaataattta aaacgtaatt taaataagaa gaaacgcagc ttaaaaacat
      721 atatcgtgag catttttctc acgatgtgtt gcatccttct ttccggctgc ggtatatctg
      781 gtacagtggg acagaattcg tcaaaggtat ccgatgaaaa agatacccgc ccattggtag
      841 tgactactat ttttccatac tatgattttg tccgccagat agcaggggac agagtaagac
      901 tgaaaatggt agttcctgca ggaatggaca gtcattcatt tgagcccaca cctgcagata
      961 tgatcaccat gcagcaggca gatctgatgg tttgcaacgg tggagagatg gaacagtggg
     1021 tggaaaaagt agtagcttcc ctggatacgg agaacatgaa agtccttacg atgatggact
     1081 atgtagatgt actggaagag gagatcgtgg aaggaatgga agataatgaa cattcccatg
     1141 atgaccatgg tcacgctcac gaaaatgagg aagaagatca tgatcacagt gaaatggatc
     1201 atagtcgtcc tgacgatgag ataaaaactc atgagaattt atctcaaatt acttttgacg
     1261 atcatgatgg tcatgaaatg gacattgaat atgacgaaca catctggacc tcaccagcca
     1321 atgccatgaa gttagtggaa gtgatcacag aaactttaaa agaagtatct ccggagaatg
     1381 cttcttattt tgaagaaaac aaaaacagct atgaaacaga actaaaaagt ctggatgatg
     1441 agtttaagga tattgtggca aatggccgtc accgtatgat cgtggtagcg gacaagtttc
     1501 cgttccggta ctttgcccag gaatatggtc tgtcttacag agcagcattt tccggctgca
     1561 gtacagatac agagccaagt gcaaaaacca ttgcttatct cattgaccgt ataaaggaaa
     1621 atcagatcca ggcagtattt tatctggaac tttccagtca ccggacggca gatatcatca
     1681 gtgaagaaac aggagcaaaa ccccttctcc ttcattcctg ccataatgta acaagaaggc
     1741 agtttgatga aggcgttacc tatctgcagc tgatgaagca gaatgcaaaa aatctgcagg
     1801 aagcactttt ataggaggaa tgatcaaaaa tggaaccatt gatcaaatgt gagcatgttg
     1861 atcttggata cgaaaatcag gatgcggtta taaatgtaaa tatggaagtc tgccctgggg
     1921 attatctgtg tatcgtaggt gaaaacgggt ctggcaaaag taccctgatc aagggacttt
     1981 tggggctgtt aaagccatcc ggcggcaaac tgaccgtagc agaagaatta aaaacaacag
     2041 gaattggcta tctgccccag cagacagcag ctcagaaaga tttccctgct tctgtaagag
     2101 aagtcgtttt aagcggctgc ttaagcagaa gaggcagacg ccctttctat tctaaaacag
     2161 aaaaggacat tgcatctgcc aatatggaaa aactgggtat tacccagttg gcgaaccagt
     2221 gctacaggga gttatccggc ggacagcagc agagagtgct gatcgcaaga gctctctgcg
     2281 ccaccaggga acttcttatt ttagatgagc cgatcaccgg tctggacccg atggcaatcc
     2341 aggatttcta ttccatgatc cgcaaattaa acagggaaga taaagtagct attatcatgg
     2401 tctcccatga cttaagaaat gcagtagagg aagccaacaa gatcctccac cttcagaaac
     2461 aggtgctttt ctacggcccg gcccatgatt atatgaacag taaggcagca ggccatttct
     2521 tccatgaaaa agagcagggc gagtgtctgc caacggcagc ctgccatatg gcaaaaaatg
     2581 tggatggtct ggaaaacaat gaacagaagt gttcctgcgg acatacccac gcacacagtc
     2641 cccagaagat agaaggagga aatacccatg aacgtgctta gtgaaatgtt atcttatcca
     2701 tttatggtgc gggcattatt cggcggtatg cttgtatctt tgtgcgcctc ccttttggga
     2761 gtgagcctgg tgttaaaacg ctattccatg atcggtgacg gactttccca tgtatccttt
     2821 ggagcccttt ccattgccct ggctatgggc tggtcccctt taaaggtatc gatcccggta
     2881 gttgtactgg cagcgttttt cctgctccgt attacggaaa acagccggat caaaagtgat
     2941 gcagccattg ccatgatctc tgccagctct ctggctatcg gtattatcgt tacatcctta
     3001 acaacaggaa tgaccacaga tgtaagcagc tatatgtttg gaagcatttt agccatgacc
     3061 aaagaagacg tggtcttatc agcagtattg tgcatcattg tactgggcct gtttgtactt
     3121 tgctacaacc aggtatttgc agttaccttt gatgagaact ttgcaaaagc aaccggtgta
     3181 aatgtaggtg cctacaatat gcttatttcc gtgcttacag ccgttaccat tgtccttggc
     3241 atgcgtatga tgggagccat gctgatctca agccttgtga tcttcccatg ccttacctct
     3301 atgcgcgtgt ttaaaagctt taccagcgta gtgatctctt ccggaattct ttccctgata
     3361 tgctttttgc tgggaatgat ggcttcctat cagttttcca cacctgcagg agccagcgtg
     3421 gtagtggtaa atctcattgc cttcggtttg ttttccatgt ggcaggtatt aggcaggaaa
     3481 cggtaaaaag cataaaatca gcataaaacc aacatgcaga ccagtgttaa tgagaaagag
     3541 gggcccatta cttgacatta aggtcaggtt gtgccaaaat atctaattga cagattgaac
     3601 aaacgggatc ataagatccc attgcaaaat gcttttctaa ttctaattta aagaaggcat
     3661 taataaagaa agaaggaaca tacgatgaca aagatcgata ttatttccgg tttccttgga
     3721 gctggaaaaa ctacttttat taa
//
LOCUS       DBMDUA010000239         3712 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8820_length_3712_cov_1.959845, whole genome shotgun sequence.
ACCESSION   DBMDUA010000239 DBMDUA010000000
VERSION     DBMDUA010000239.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8820_length_3712_cov_1.959845"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="ACZPCL_12645"
     CDS             <1..1404
                     /locus_tag="ACZPCL_12645"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1163351.1"
                     /translation="ITIRPAASFKDNNTLFRVPARLDPDTEDLFYPASDLTDISSITW
                     RSDTGQQIIQMIRPIESTRNFHHMGTLYISLYSSYIDNLVKNIHFDEKGFALILDSKN
                     QPINIKEVDPSFLTDLDPFLTGTNGCFNRKIGSVDYEYFYTTSPKTGWKSLGIISVSD
                     LHAQVRKLALSVITGVLLVSAVAIFISYRLSRSFAEKIHTVTAAMKKASEGDFSVNLA
                     EGISKNEFNDLNTGFNHMIQKINSLIQTVFKAEPLRKEAEYASLQAQTNPHFLYNTLD
                     TICWQAKLAGNDAIFDTTYSLASLLRASMGNTDPYITVSQELSYIRDYIQIQKARFRD
                     KIQANIAVEPALMPVMIPKLILQPIVENAFIHGLEEKNGNGTIKISGILNDEEETVLF
                     MIHDNGVGMTRAQIEKALDPPPGTKGSFGLSSVHKRLQLLYGTDFGLKILSDPGKGTT
                     IIIRMPLHVEKAEKEKQ"
     gene            1428..2990
                     /locus_tag="ACZPCL_12650"
     CDS             1428..2990
                     /locus_tag="ACZPCL_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1163352.1"
                     /translation="MYKIMIADDEPIVLDSLKTFPWSDHQCCVTCFAENGLNALHTLE
                     SEKVDILISDIRMPGMNGLELSKAVKERSPETEIILLTGYAEFEYAKQALSLGIRQYL
                     LKPFRFEDIESALLSCIHSLDNLESRRRQQTYIQEKLQLISPLLTEQIYQDLLEGRIG
                     DYSEKIAACNIKDALYVVISTQSDFPGSSLDIALYAQIKELLNGMEPEVYLAQGIDII
                     SCILCFNAKHSPEFCEVASLHLCEMLQKTTLQELGFHISFGISLPSSDIFMLHQLKKQ
                     SVQALNCRSALGGNSLIMYSEIQKKQDHDIFNLTMYEKKIQKCIVQNQKQELQQTFRQ
                     FFDELLQSAHGDFSYIKKTLINLVVLTYRFAESYINSSENEYDAIEHLFRCESMETLS
                     KESLSLLESLIQTGSSSFGDGIAERITDYLEQNLEQNVSLDMLAQSMNYSAAYLSRLI
                     KKNTGQSFSELLLDLRLKKSMELLRLTDMRINDIAAKAGYNDVSYFISIFKKCTGVTP
                     KEWRSLAKLGEL"
     gene            complement(3051..>3712)
                     /locus_tag="ACZPCL_12655"
     CDS             complement(3051..>3712)
                     /locus_tag="ACZPCL_12655"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HIR1163353.1"
                     /translation="LHNAIKEGKKILLEGQLGSLKDPDHGIYPMVTSSSTLAAYGAIG
                     AGIPPYEIKDITTVVKAYSSAVGAGAFVSEIFGDEAEELRNRGGDGGEYGATTGRPRR
                     VGWFDAVATRYGCRIQGATEVAFTVLDVLGYLDEIPVCIGYEIDGEVTRDFPTTAKLE
                     KAKPVFTKLPGWKTDIRGIKNYADLPENCRKYIEFVEKEIEVPITMVSNGPGRDDIIL
                     K"
BASE COUNT         1051 a          841 c          752 g         1068 t
ORIGIN      
        1 attaccatcc gccccgctgc ttcatttaaa gacaataata ccctttttcg tgtacctgcc
       61 aggctggatc cggatacaga ggatctattt tacccagctt ctgacctgac tgatatatcc
      121 tcaattacct ggcgcagtga taccgggcaa cagatcatcc agatgatacg gcccattgaa
      181 agtaccagga attttcatca tatgggcacc ttatatattt ctctctacag cagctatatt
      241 gacaatctgg taaagaatat ccattttgat gagaaaggat ttgcccttat cttagattcc
      301 aaaaaccagc ctattaatat caaagaagtg gatccttctt tccttacaga tcttgatcct
      361 tttcttaccg gcacaaatgg atgttttaac cgtaagatcg gctctgtgga ttatgagtat
      421 ttttatacta cttctcctaa gaccggctgg aaatccctgg ggatcatttc agtttctgac
      481 ctgcatgccc aggtacgaaa actggctctt tctgttatta caggcgtact tttagtatct
      541 gctgtggcaa tatttatttc ctacaggctt tcacgctctt ttgctgaaaa aatccataca
      601 gtaacagctg ccatgaaaaa agcctcagaa ggcgatttct ccgttaatct ggcagaaggc
      661 atttcaaaaa atgaatttaa cgacttaaat accggtttta atcacatgat ccagaagatc
      721 aattccctga tccagactgt attcaaggca gagcccttgc gcaaggaagc agaatatgct
      781 tccctgcagg cacagacgaa tccacatttt ctttataata ccttagatac catctgctgg
      841 caggcaaaac ttgcaggcaa cgatgctatt tttgatacta cctattctct ggcctctctc
      901 ctgcgtgcat ctatgggaaa cactgatcct tatataactg tttcccagga actgtcttat
      961 atccgtgatt atatacagat acagaaagcc cgcttcaggg ataaaataca ggcaaatatt
     1021 gctgtagaac cggcacttat gcctgttatg atccccaaac ttattcttca gcctattgtg
     1081 gaaaatgcct ttatccatgg tctggaagaa aaaaatggaa acggtactat aaaaataagc
     1141 gggatcttaa atgatgagga agaaaccgtc ctctttatga tccatgataa tggtgtggga
     1201 atgacacggg cccagataga aaaggcctta gatccgcctc caggcacgaa aggcagcttt
     1261 ggactttcca gtgtccacaa acggctccag cttctgtatg gaacggattt tggcttaaag
     1321 atcctgtctg atccaggaaa aggaaccact attatcatcc gcatgcctct tcatgttgag
     1381 aaggcagaaa aagaaaagca gtaaagaatt tgaggaaata tgtatgtatg tataagataa
     1441 tgatcgcaga cgatgaacct atcgtacttg acagtttaaa aacatttcca tggtctgatc
     1501 accagtgttg tgtgacctgc tttgcagaaa acggcttaaa tgcactgcat actctggaat
     1561 cagaaaaagt ggatattttg atcagcgata tacgtatgcc ggggatgaac ggcctggagc
     1621 tttccaaagc tgtaaaagaa cgatccccgg aaacagagat catactttta accggctatg
     1681 cagaatttga atacgccaaa caggctttat cacttggaat ccgccagtac cttttaaagc
     1741 cattccgatt tgaagatatt gaatccgccc tcctttcctg tatccattcc ttagataacc
     1801 tggaaagcag gcgcagacag cagacataca tccaggaaaa gcttcagctc atctctcctc
     1861 ttctcacaga gcagatctac caggatctat tagagggacg tattggggat tattcagaaa
     1921 aaatagctgc ctgcaacatt aaggatgctc tttatgtggt catttccacc caaagtgact
     1981 ttcccggaag ttccttagat attgccctct atgcccagat caaagagctg ttaaatggaa
     2041 tggaaccgga agtttatctt gcccagggca ttgatattat cagctgtatc ctttgtttta
     2101 atgcaaagca ttcccctgaa ttttgtgagg ttgcctcttt acacctgtgc gaaatgctcc
     2161 agaagacaac gctgcaggaa ctggggttcc atatttcttt tggcatcagc cttccaagtt
     2221 ctgatatttt tatgcttcat cagttaaaaa agcaatctgt ccaggccctt aactgccgga
     2281 gcgctttagg tggaaattcc ctgatcatgt attcggaaat acagaaaaaa caggatcatg
     2341 atattttcaa tctgaccatg tatgaaaaga aaatacagaa atgtattgtt cagaatcaga
     2401 aacaagaatt acaacagacc ttccggcagt tttttgatga actgctccag tctgcccacg
     2461 gtgacttttc ttatattaaa aaaacgctga tcaatcttgt tgtgcttaca taccgttttg
     2521 ccgaaagtta tattaacagt tctgaaaatg aatatgatgc tatcgaacat ttattccgct
     2581 gtgaaagtat ggaaacattg tccaaagaat ctctttccct gttagagtct ctgatccaga
     2641 caggatccag ttcttttggc gatgggattg cagaacgcat cactgattat ctggaacaga
     2701 atctggagca gaatgtttct cttgatatgc tggcccagtc tatgaattac agcgccgctt
     2761 atctgtcccg cctcatcaaa aagaatacag gacagtcctt cagcgaactg cttttagacc
     2821 tgcgtttaaa aaaatccatg gagcttttac ggcttacgga tatgcggatc aatgatattg
     2881 ctgccaaagc aggctacaac gatgtaagct atttcatctc tatttttaaa aagtgtaccg
     2941 gcgtcacacc taaggagtgg cgcagcctgg caaagctggg ggagttatag aatgttatta
     3001 aaaaacaggc gcatcctgat ctttcggata cgcctgtttt ttccgttcat ttatttaagg
     3061 ataatatcat cacggcctgg gccattagat accattgtaa tcggtacttc aatctctttt
     3121 tctacaaact caatatactt acggcagttc tctggaagat ctgcgtagtt cttgattcca
     3181 cggatatcag tcttccatcc tggcagcttg gtaaatactg gtttagcctt ttcaagcttt
     3241 gcagtagttg ggaagtctct ggttacttcg ccgtcgatct cgtagccgat gcaaaccggg
     3301 atctcatcta agtagccaag tacatccagt acagtgaaag ctacttctgt agcgccctgg
     3361 atacggcagc cgtatctggt agcaactgca tcgaaccagc ctactcttct tggacgtccg
     3421 gtagtagcgc cgtactcgcc gccgtcaccg ccacggtttc ttaactcttc tgcttcatca
     3481 ccgaaaatct cgctgacaaa tgcgcctgca ccaactgcgc tggagtatgc cttaacaaca
     3541 gtggtaatgt ccttgatctc atatggtggg atacctgcgc cgatagcacc gtaagcagcc
     3601 agtgtagagg aagaagttac cattgggtaa attccgtgat ccggatcctt taaggaacct
     3661 aactgtccct ctaacaggat cttcttgcct tctttaatgg cattgtgcag at
//
LOCUS       DBMDUA010000240         3709 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8826_length_3709_cov_1.930155, whole genome shotgun sequence.
ACCESSION   DBMDUA010000240 DBMDUA010000000
VERSION     DBMDUA010000240.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8826_length_3709_cov_1.930155"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1644)
                     /locus_tag="ACZPCL_12660"
     CDS             complement(<1..1644)
                     /locus_tag="ACZPCL_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HIR1163354.1"
                     /translation="MKRLLSYSVVMFVITIILLLIIPLPAGLVDVAIILNMSLSMMIL
                     VITMTIREPLEFSIFPSLLLITTLFRLGINVSTTRNILSKGGSSGRVIAAFGDFVLRG
                     NVVVGLIIFLIIVLMQFIVITKGAERVAEVAARFNLDAMPGKQMAIDADLSSGLINET
                     QAKERRAKVQREADFYGAMDGATKIVKGDAVMSLITTAINLIGGSIIGIVQSGSSISA
                     VLSTYSIATVGDGLVSQIPALLISTATGMIVTRAVSEGSLNEDVSKQFLAQPHAMIVS
                     GAAVAVMAVIPGMPAVRTLVVAACLMGSGFYLSGKIKKEPQALSQMAMDPSTQLQDSP
                     QAQAAAAKEEVSEEEYFKDVNNVYSLLTLEPVEIEFGYSLIPLADESVGGRLISRIVI
                     FRRQYAQDMGFVIPSIRLRDSSSLGPSQYSIRIKGEEIAGGEILIDYFLALEPEEVEE
                     EIEGIEAIEPAYGIPSRWIKPENRDRAELYGYTVIDPLSVLLMHLSEVIRQHSYELLT
                     RQEVVRLTENLKKTAPELVEEAIPRILINSSSSELHIKCV"
     gene            complement(1666..2739)
                     /gene="flhB"
                     /locus_tag="ACZPCL_12665"
     CDS             complement(1666..2739)
                     /gene="flhB"
                     /locus_tag="ACZPCL_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HIR1163355.1"
                     /translation="MGQSGQEKTEQPTAKKRRDARKEGNIFQSRDVSTVVILAGIFFG
                     IRIWLPFIYGQMKNYMTWVIDGAARSSEDMLSASLVFVTLSVFLKCALPLLVLALLLG
                     IVAHGVQTRFNVSFKSIRPKWNRLNPLSGIKRLFSMKNIVELVKNSIKITLLLVLLYN
                     ILKQDLQPMASMIDMNPAASAAYMMKMLFDLLVRVCIAFTVIAFGDYLYQRWEYEKEL
                     RMTKQEVKDEYKQTEGNPEIKNRIKSLQRQMASSRMMQKVPQADVIIRNPTHVAIALK
                     YDPDHDNAPVVLAKGLDELALRIVKVGEENNVYTIENKPLARAMYNSCELDRPIPSEF
                     YTAVAEILVYLYKQDNGKNKDRK"
     gene            complement(2757..3515)
                     /locus_tag="ACZPCL_12670"
     CDS             complement(2757..3515)
                     /locus_tag="ACZPCL_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HIR1163356.1"
                     /translation="MFITFSMAVMRMSGAIGFNPLFGKSNFPVKIRAYFVFALALLLY
                     LCADQTKIAYPESMAEYILMLAGELFLGAVLAFAMELAFLTIRFASSIMDYSMGLSMA
                     QVYDPQYGTQSTVSSGIYYGFMVLLFFASNSHLRLLALFYQSVQLIPFGKVTFNPMLV
                     YVITDGFCQGILTGMQFAFPMIAIELVTELAMGILMRIIPQINVFTVNFQVKIIMGLL
                     MMLLLFSPMADYIDTIFTYMYQMMWKLLSNMKPV"
     gene            complement(3525..>3709)
                     /locus_tag="ACZPCL_12675"
     CDS             complement(3525..>3709)
                     /locus_tag="ACZPCL_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HIR1163357.1"
                     /translation="SMIVGVVIAVFQAATQINEQTMTFVPKLLAIMVLLGFLGSSMLV
                     MLQEFFQYIVELIAKG"
BASE COUNT         1058 a          895 c          716 g         1040 t
ORIGIN      
        1 gacgcatttt atgtgcaact ccgaagaaga actgttgatg agtatccgtg gtattgcctc
       61 ttccaccagc tcaggagctg tttttttcag attttccgta agccttacca cttcttgtct
      121 tgtaagcagt tcgtatgaat gctgccggat cacttctgaa agatgcatga gcaatacaga
      181 cagaggatca atgaccgtat aaccatataa ttcagcccga tctctgtttt caggcttgat
      241 ccacctgctg ggtataccgt atgccggttc aatggcttca ataccttcaa tttcttcttc
      301 cacctcctct ggttccagag caaggaaata gtcaatcagg atctctcctc ctgcaatttc
      361 ttctcctttt atgcggatac tatactgact tggtcccagg gaagaactgt cccggagccg
      421 gatggatggg atcacaaatc ccatatcctg ggcatattgg cggcggaata taacgatcct
      481 gctgatcagc cttccgccta ctgactcatc tgccagaggg atcagactat aaccaaactc
      541 gatctccact ggctccagtg taagaaggct gtatacatta tttacatctt taaaatattc
      601 ttcctcactt acctcctctt ttgcagcagc tgcctgggcc tgtggggaat cctgaagctg
      661 ggtagaagga tccattgcca tttgtgacaa tgcctgtggc tcttttttta tctttccgga
      721 aagataaaat ccgcttccca taagacatgc agccacaacc agagtcctta ctgccggcat
      781 tccaggtatc acagccataa ccgccactgc tgcaccacta acgatcatag catgaggctg
      841 ggccagaaac tgctttgata catcttcatt taagctgcct tctgaaacag ctcttgtaac
      901 gatcattcct gttgcagtag aaataagaag agccggtatc tgggatacca gaccatcacc
      961 tactgtcgca atggaatagg tactcagcac agctgagatg ctgctgccgg actgtacaat
     1021 accgatgata ctgccgccga tcagattaat ggcagttgtg ataagtgaca taactgcatc
     1081 gccctttacg atcttagtcg caccatccat tgcaccataa aaatctgctt ctctctgaac
     1141 cttggcacgc cgttcctttg cctgtgtttc attgatcagg ccagaactaa ggtctgcatc
     1201 aatagccatc tgttttcccg gcatggcatc cagattaaat ctggctgcta cctcggcaac
     1261 tcgttctgca cctttggtga taacgataaa ctgcatcagc acgatgatca ggaagatgat
     1321 cagacctact actacattcc ctcttaaaac gaaatcacca aaggctgcaa tgacacggcc
     1381 tgatgaacct cccttagaaa ggatatttct cgtggttgaa acattgatgc ccagacggaa
     1441 cagcgtggta ataagtaaaa gagaggggaa tatggagaat tccagtggct cccgaatggt
     1501 catggttatt accaggatca tcatggagag tgacatattc aatatgatcg ccacatccac
     1561 cagtcccgcc ggaagaggaa tgatcaaaag caatatgatc gtgatcacaa acataaccac
     1621 tgagtaactc aataaccgtt tcattcgtcc attaatctcc aactattatt ttctatcttt
     1681 atttttcccg ttatcctgtt tatacagata tacaaggatt tctgctactg ctgtataaaa
     1741 ttctgatggg atcggcctgt ccagttcaca ggaattgtac atagcccgtg caagtggttt
     1801 attttcaatg gtataaacat tgttttcctc ccccaccttt acgattctta acgccagttc
     1861 atctaatccc ttggcaagta ctaccggagc attgtcatga tccggatcat attttaaagc
     1921 aatggccaca tgggtaggat ttcgtatgat cacatctgcc tggggaacct tctgcatcat
     1981 cctgctggat gccatctgac gctgcaggct tttgatcctg tttttaatct caggattacc
     2041 ttctgtctgt ttatactcat cttttacttc ctgctttgtc atacgaagct ctttttcata
     2101 ttcccagcgc tgatacagat aatcgccaaa agcaatgacc gtaaaggcaa tacatacacg
     2161 taccagcaga tcaaatagca ttttcatcat ataggctgca gatgctgcag gattcatatc
     2221 gatcattgat gccatgggct gcagatcctg cttcagtatg ttgtaaagca ataccagaag
     2281 cagtgttatc ttgatggaat tttttaccag ttccactatg tttttcatgg aaaacagccg
     2341 ttttatacct gaaagaggat taagccggtt ccatttcggc cggatggatt taaaggatac
     2401 attaaaacgg gtctgtactc catgcgccac aattccaagt aaaagggcta aaaccaacag
     2461 aggaagcgca cattttaaaa acacagacag tgtaacaaaa acaaggctgg ctgaaagcat
     2521 atcttctgag gatcgtgctg ccccatctat gacccatgtc atataatttt tcatctgtcc
     2581 atatataaaa ggaagccata tgcggatccc gaaaaatatg ccagccagga ttactacggt
     2641 agatacatcc ctgctttgaa agatatttcc ttcttttctg gcatcccgcc gttttttggc
     2701 ggtaggctgt tctgttttct cctgaccact ctgtcccatc ttctgttaca ctcccttcag
     2761 actggtttca tattgcttaa aagcttccac atcatctgat acatatatgt aaagatggta
     2821 tctatataat ctgccattgg cgaaaacaac agaagcatca tcaaaagccc cattatgatc
     2881 tttacctgga aatttactgt aaaaacattg atctggggaa tgatacgcat caggatcccc
     2941 attgccagtt ctgtcacaag ctcaattgct atcattggaa atgcaaactg cataccagta
     3001 aggatcccct gacaaaatcc atctgtgatc acatagacca gcatgggatt aaatgtcact
     3061 tttccaaaag ggatcaactg tactgactgg taaaacaatg ctaacagccg taaatgactg
     3121 ttgctggcaa aaaagagcag taccataaat ccataataaa taccggaact gaccgttgac
     3181 tgggttccat actggggatc ataaacctgg gccatgctaa gtcccattga ataatccata
     3241 atggaagaag caaaccggat cgtaaggaac gccagttcca ttgcaaaggc taacactgcc
     3301 ccaagaaaaa gctctcctgc aagcatcagg atgtattctg ccattgactc cggatatgct
     3361 atcttagtct ggtctgcaca cagatacagg agcagagcca gggcaaaaac aaaatatgcc
     3421 cttattttta caggaaaatt actttttcca aaaagtggat tgaatccgat ggctccgctc
     3481 atacgcatca ctgccattga aaatgtgata aacatagatc ttttttaccc ctttgcgatc
     3541 aattccacta tgtattgaaa aaattcctga agcataacca gcatagaact tcctaaaaaa
     3601 cctaaaagca ccataatagc cagcagtttg ggtacaaatg tcatcgtctg ttcattaatc
     3661 tgtgtagctg cctgaaatac agcaataacc actcctacga tcatactga
//
LOCUS       DBMDUA010000241         3659 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8969_length_3659_cov_2.482293, whole genome shotgun sequence.
ACCESSION   DBMDUA010000241 DBMDUA010000000
VERSION     DBMDUA010000241.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8969_length_3659_cov_2.482293"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /gene="cmk"
                     /locus_tag="ACZPCL_12680"
     CDS             complement(<1..655)
                     /gene="cmk"
                     /locus_tag="ACZPCL_12680"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR1163358.1"
                     /translation="MNHFNIAVDGPAGAGKSTIAKLVAKELGFVYVDTGAMYRAMALY
                     FLRKGVDPKAAEEIERSCPEVDVTIRYQDGVQQVLLNGEDVSGLIRTEEVGNMASAVS
                     GYMPVREKLVELQKQLAAKENVIMDGRDIGTCVLPDAPAKIYLTASVEVRAARRYKEL
                     LEKGMKADLKEIEKDIEDRDYRDMHREHSPLKQAEDAVLLDTSSMTLAQVVEEILKIV
                     "
     gene            complement(729..2009)
                     /locus_tag="ACZPCL_12685"
     CDS             complement(729..2009)
                     /locus_tag="ACZPCL_12685"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BaiN/RdsA family NAD(P)/FAD-dependent
                     oxidoreductase"
                     /protein_id="HIR1163359.1"
                     /translation="MSNQRNIIVIGGGAAGMIAAIAAAKEGCAVSLYEKNEKLGKKIF
                     ITGKGRCNVTNAGDMDELFAAVITNKKFMFSSFYGFTNEDMMQFLEDAGLRLKTERGK
                     RVFPLSDHSSDVIAALDRTLKKENVKVYLRKEVKGLNFITEEDKTICKGIFLEENGKK
                     TAVDADCVIVATGGMSYPSTGSTGDGYQWAQAAGLKVTALSPALVPFETAELEIVKSL
                     QGLSLKNVEATVSNGKKELYRDFGEMLFTHFGVSGPLMLSASSFCAKAIAKNLLKLSI
                     DLKPALTEEQLDERILRDFAEAKNKQFKNSLNHLYPAKLVPVIIERSGIDPDKQVNEI
                     TKEERRRLVETTKALTFTLTGLRPFKEAIITQGGVDVKGINPSTMEAKKAENLYFAGE
                     VLDVDAVTGGFNLQIAWSTGWAAGKAAAEKIQES"
     gene            complement(2134..3492)
                     /locus_tag="ACZPCL_12690"
     CDS             complement(2134..3492)
                     /locus_tag="ACZPCL_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1163360.1"
                     /translation="MNTASKTRMTEGSISQKIIFFAIPLLLGNLFQQLYNTADSLIVG
                     NFLGSNALAAVSSSSNLIFLMVGFINGIAMGAGVVIARYYGAKNSDNLHKAIHTTVAF
                     GFVAGSVLTVVGMYFAPKILVLMGTPAEVLPESVAYFRTYFAGSLGVVMYNIFVGILQ
                     SVGDSRHPLIYLICSSCINVVLDIFFIAGLGKGVGSAALATAISQFTSAILCMIHLIR
                     TKEEYQLQVGKIRFDGPSLSQIIQNGVPSGFQNSVIALANVFVQSNINAFGKMAMAGC
                     GSYSKIEGFVFLPVTCFNMALTTFVSQNLGAKQYDRAKKGAWFGILCSMSIAELIGFA
                     IYAVSPVLIAAFNSDPEVVHYGVMQAKTISLFYCVLAFSHCIAAVLRGSGNASVPMVV
                     MLCVWCLFRVSYITVAVHFIPDIRMVFWAYPLTWSISSVIFLYLFLRGKWIYGFEKNA
                     KT"
BASE COUNT          981 a          915 c          684 g         1079 t
ORIGIN      
        1 ggacgatctt taaaatctct tctactacct gggccagagt catagaagaa gtatctaaca
       61 acactgcatc ctctgcctgc tttaacggag aatgttctct gtgcatatcc ctgtaatctc
      121 tgtcttcaat gtccttttcg atctctttta aatctgcttt cattcccttt tctaacaatt
      181 ccttataacg gcgagctgct cttacctcta cagaagctgt aagatagatc tttgcaggtg
      241 catctggaag aacacaggta ccaatatccc ttccgtccat gatcacattt tcctttgctg
      301 ccagctgttt ctgaagctct accagttttt ctcttacagg catatagccg gatactgcag
      361 atgccatgtt tcctacttct tctgtacgga tcaagccgga aacatcttca ccatttaaaa
      421 gtacctgctg cacaccgtcc tggtaacgga tagtcacatc tacttccggg catgagcgct
      481 cgatctcttc tgctgccttt ggatctacgc cctttcttaa gaaataaagg gccatagcgc
      541 gatacattgc ccctgtatct acgtatacaa agccaagttc ctttgccacc agctttgcaa
      601 tggtgctttt tcctgctcct gcaggtccgt ctactgcaat attaaaatgg ttcatctggg
      661 tgttctcctt taactttcat atagcaatac cgcctgtctg gcgatctgtc tgttttattt
      721 aattttgttt agctttcctg tatcttctcc gctgccgcct ttcccgctgc ccagccggta
      781 gaccaggcga tctgcagatt aaatccgccg gtcactgcat ccacatccag gacttctcct
      841 gcaaagtaca gattttctgc ctttttcgct tccatagtag acggattgat cccctttaca
      901 tctacgccgc cctgtgtaat aatagcctct ttaaacggcc gaagccctgt cagtgtgaac
      961 gtcagcgctt tggtagtttc taccaggcgg cggcgttctt ctttggtgat ctcatttacc
     1021 tgcttatccg ggtcaatgcc actgcgctct atgatcacag ggaccaattt ggcaggatat
     1081 aaatggttta agctgttttt aaactgttta ttctttgcct ctgcaaaatc ccttaaaatg
     1141 cgctcatcca gttgttcctc tgtaagagcc ggttttaagt cgatagacag ctttaacagg
     1201 ttcttggcaa tggccttggc acagaaagaa ctggcgctta acataagagg gccgctgact
     1261 ccaaaatgtg taaacagcat ttccccaaag tcacggtaca gttccttttt gccgttagat
     1321 acagtagctt ccacattttt aagagaaagg ccctgaaggg attttacaat ctcaagctct
     1381 gctgtctcaa atggcactaa ggctggtgaa agggctgtta ccttaagtcc tgcagcctgg
     1441 gcccactgat aaccatctcc tgtggatcct gtagatggat aagacattcc gccggttgct
     1501 acgatgacgc aatccgcatc cacagctgtc ttttttccat tttcttccag gaaaatgcct
     1561 ttgcagatgg tcttatcttc ttctgttata aagtttaaac cttttacttc cttgcgaaga
     1621 tatactttta cattttcttt ctttaatgtg cgatctaagg ctgcgatcac atcggaagaa
     1681 tgatccgata aagggaatac cctctttcct ctctctgttt tcaggcgcag tcctgcatct
     1741 tccagaaact gcatcatatc ttcattggta aagccgtaaa agctgctgaa cataaatttc
     1801 ttattggtga tcacagctgc aaacagctcg tccatatcac cggcatttgt cacattgcag
     1861 cggccttttc ctgtaataaa gatctttttg cccagctttt catttttttc atagaggctt
     1921 accgcacagc cttcctttgc tgctgcaata gccgcgatca tacctgcggc accgcctccg
     1981 atgacgatta tatttctttg gttgctcatg tattgttgtt tcctttatta tatatattcg
     2041 cgtttcatgc atcctgtttc atagaatatt gaacctgtct gttagtataa catacttatt
     2101 tataaatttc attcatatct tatatccaga tattcaggtc tttgcatttt tttcaaaacc
     2161 atagatccat tttccacgga gaaacaaata caggaaaata acagaactga tgctccaggt
     2221 cagaggatac gcccagaata ccatacggat atccgggata aaatggactg ccactgtaat
     2281 ataacttaca cggaataaac accatacgca aagcataact accataggaa cagaagcatt
     2341 tcctgaaccg cggagcacag ctgcaataca atgtgaaaaa gccagcacac aataaaacag
     2401 cgaaatggtt tttgcctgca taacgccata atggactacc tccgggtcgc tgttaaaagc
     2461 tgcgatcaga accggagata ctgcgtaaat tgcaaagccg atcagttccg caatggacat
     2521 agaacaaagg ataccaaacc atgccccttt ttttgcacgg tcatactgct tcgcacctaa
     2581 attctgactg acaaaagtgg tcagtgccat attaaaacag gttacaggca gaaatacaaa
     2641 tccctcgatt ttagaatatg agccacagcc tgccattgcc attttcccaa atgcgttgat
     2701 atttgactgt acaaacacgt ttgcaagagc gatcacagag ttctggaagc cggaaggtac
     2761 accattctga atgatctggc tcaaactcgg tccatcaaaa cggatcttac caacctgcag
     2821 ctggtactct tcttttgtcc gtatcaaatg gatcatacaa aggatcgcac ttgtaaactg
     2881 ggaaatagct gtcgcaaggg ccgctgaccc aacacctttg ccaagacctg caataaaaaa
     2941 tatatccagc acaacgttga tgcaggaaga acagatcaga tagatcagcg gatgacggct
     3001 gtcacctaca gactgcaaaa ttcctacaaa aatattgtac ataacaactc caagggagcc
     3061 tgcaaaatac gtgcgaaaat atgccactga ctcaggaagc acctctgccg gcgttcccat
     3121 caaaaccagg atcttcggcg caaaatacat acccactaca gtcagcacac ttcctgccac
     3181 aaaaccaaaa gccactgtgg tatggatcgc cttgtgcagg ttgtcactgt ttttcgcccc
     3241 ataatacctt gcaatgacaa caccggctcc cattgcaata ccattaataa atccaaccat
     3301 taaaaagatc agattgctgg atgagctgac cgccgccagt gcattacttc caagaaaatt
     3361 ccccacgatc agtgaatccg ccgtattata cagctgctga aacaggtttc ctaaaagcag
     3421 tggaatggca aaaaagatga tcttttggga gatgcttcct tctgtcattc ttgttttgct
     3481 tgctgtgttc attgtgtgtg ccccatcctt ttgtatttta attgatttaa tttgcagatc
     3541 atcctgccga ctgcaatatc cctttcaata tccgcaaatt ataaacattt taccgcaata
     3601 tatcaatttt gtttggatat attgcggcaa aatattaagt ccccaggata tcttgcgaa
//
LOCUS       DBMDUA010000242         3658 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_8970_length_3658_cov_3.806016, whole genome shotgun sequence.
ACCESSION   DBMDUA010000242 DBMDUA010000000
VERSION     DBMDUA010000242.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8970_length_3658_cov_3.806016"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(127..870)
                     /gene="sufC"
                     /locus_tag="ACZPCL_12695"
     CDS             complement(127..870)
                     /gene="sufC"
                     /locus_tag="ACZPCL_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR1163361.1"
                     /translation="MAKELLDVKQLFVNVEDKEILHGVDLKIGKGETHVLMGPNGAGK
                     STLGYALMGNPRYQLTNGEIWFQGKKINEEPVDKRAKAGMFLSFQSPLEVPGLSLSSF
                     IRSALEQRRGERIRLWDFRKELAKAMELLQMDPSYADRDLNVGFSGGEKKKAEILQML
                     MLKPNLAILDETDSGLDVDAVRTVSKGVEEYQKDRDGSLLIITHSTRILEALHVDYTH
                     VMVDGKIVKTGDGSLVDQINETGFEEFLK"
     gene            1135..1548
                     /locus_tag="ACZPCL_12700"
     CDS             1135..1548
                     /locus_tag="ACZPCL_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1163362.1"
                     /translation="MIVSTKGRYALRVMVELAQHQGDGYVPLNVIAERQDISEKYLES
                     ILKVLVQDKLLTGLRGRGGGYRLTKAPEDYTLGEILRLTEGSLAPVACTDDGEHDCPR
                     KGICPSHPVWQKLDNMINEFFDGITLADLIQTAPQ"
     gene            complement(1542..2141)
                     /locus_tag="ACZPCL_12705"
     CDS             complement(1542..2141)
                     /locus_tag="ACZPCL_12705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR1163363.1"
                     /translation="MVSRIEVGWRQGEKEGTIPVDVVYSKRRTIGLEVQADGKVLIRA
                     PKGITKKVLMDFVNDRQEWIVEKWFLIEKHRRKKAEAPKPDYEGNPEVEAFYRRAARK
                     KIIERTAWYAKIMGVTYGRISIRAAKTRWGSCSGEGNLNFHWKLILMPPEILDYVVVH
                     ELAHRKQMNHSPLFWAEVEKVLPDYKARRAWLKENGRDY"
     gene            complement(2315..3127)
                     /locus_tag="ACZPCL_12710"
     CDS             complement(2315..3127)
                     /locus_tag="ACZPCL_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1163364.1"
                     /translation="MNYALSLLKEIIKKRKLVWDLGKADFRKRFVGSYFGIVWMFIQP
                     VVTIVIYAVIFGIGFKSPPPVPGVSYVIWLISGIVPWFFFGEALNGITGCLQEYSYLV
                     KKVVFKVEILPMIKLISCLLVHGFFVLIMMIMYLVAGRMPMLSWIQVIYYSFAASMLA
                     LGLGYFTSSVNVFFKDMSQIVGICLQFGIWMAPIMYHESMFTNRLPWIEPLFKINPFY
                     YIVTGYKDSMITGNWFWERPKLTIYYWVFTLLVFGVGLKMFKKLRPHFSDVL"
     gene            complement(3167..>3658)
                     /gene="rfbC"
                     /locus_tag="ACZPCL_12715"
     CDS             complement(3167..>3658)
                     /gene="rfbC"
                     /locus_tag="ACZPCL_12715"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR1163365.1"
                     /translation="FKDERGYFMETYNQNDFKEAGLNMTFVQDNQSMSVKGVLRGLHF
                     QKQYPQGKLVRAVRGTVFDVAVDLRSDSKTYGKWFGVTLSAENKKQFYIPEGFAHGFL
                     VLSDEAEFAYKCTDFYHPGDEGGLLWSDPEIGVDWPIEPGMELIISDKDKKWSGLKDT
                     FKF"
BASE COUNT          982 a          921 c          684 g         1071 t
ORIGIN      
        1 ttctgtctat atcattgatc tgtgttttta cttccatatg ctttcgattt cctttccgga
       61 aactgtttat aatctgactt ataatctact ttgtaaactg cctaagggca tatttacatt
      121 ttccttttac ttaagaaatt cctcaaatcc ggtctcattg atctggtcta ccagagagcc
      181 gtctcctgtt ttaacgatct ttccatctac cattacatga gtgtaatcta catggagcgc
      241 ttccaggata cgggtgctgt gggtgatgat cagtaaagag ccatctctgt ctttctgata
      301 ctcttccacg ccctttgaaa cagtgcggac tgcatctacg tccagacctg agtctgtctc
      361 gtctaagatt gccagatttg gctttaacat aagcatctgc aggatctcag cctttttctt
      421 ctcaccgccg gaaaaaccca cattcagatc tcggtctgca taagacggat ccatctgcaa
      481 aagttccatt gcctttgcca gctcttttct gaaatcccag agacggatgc gctctcctct
      541 tctctgctct aaagcactgc ggataaagga acttaatgac agtcccggta cttccagtgg
      601 gctctggaag gataagaaca tgccagcttt agctctttta tctaccggct cctcgttgat
      661 cttctttccc tgaaaccaga tctcaccatt tgtcagctga tatcttggat ttcccattaa
      721 tgcatagcct aaagtggact tacctgcacc atttggtccc attaatacat gggtctctcc
      781 ttttccaatc tttaagtcta ctccatgaag gatctcttta tcttctacat ttacaaataa
      841 ctgttttaca tccagtaatt cctttgccat gaccgtactt cctttctcat catattctcc
      901 catgatcagg aggacatttt tataaaccta ctcatgtaat tggtttattt agcacattta
      961 taatagtatt ataaacctag tttgtcaata ggtattaatt ttcatatcag cttgctaagt
     1021 atatgctttc tggtgtatac tatatggtaa tcttcaaaga gatagaaaga tatgtgttta
     1081 aagaattact ataaataact ataataacta ttttgaataa aggagaattc gataatgatc
     1141 gtttctacaa aaggccgtta tgccctgcgg gtcatggtag aactggccca gcatcaggga
     1201 gacggctatg ttcctctgaa cgtgatcgcc gaacggcagg acatttctga aaaatatctg
     1261 gaaagtatat taaaggtact ggtacaggat aaacttctca ccggactccg gggccgtggc
     1321 ggcggctacc gcctgacaaa agcaccggaa gattatacgc tgggtgaaat actccggctc
     1381 accgaaggtt ccctggctcc cgtagcctgc accgatgatg gcgaacacga ctgccccaga
     1441 aaagggatct gcccctccca tccggtatgg cagaaactgg acaatatgat caatgaattt
     1501 tttgacggga tcacattggc ggatctgata cagactgcgc ctcaatagtc tctcccgttt
     1561 tccttcaacc atgcccgtct tgccttataa tctggcagca ctttttctac ttctgcccag
     1621 aaaaggggcg aatggttcat ctgttttctg tgggccagtt cgtggactac tacatagtct
     1681 aagatctccg gcggcatgag gatcagtttc cagtgaaagt ttaagtttcc ttccccgctg
     1741 cagctgcccc agcgtgtttt tgcagcgcgg atgctgatgc ggccgtaggt gacacccatg
     1801 atctttgcat accaggcagt tcgctctatg atcttctttc tggcagcacg gcggtaaaag
     1861 gcctctactt ccggatttcc ctcataatct ggctttggag cctctgcctt tttgcggcgg
     1921 tgcttctcga tcaggaacca tttttccacg atccattcct gacggtcatt tacaaaatcc
     1981 atcagcactt ttttagtgat cccttttggt gcgcggatca gcacttttcc atctgcctgc
     2041 acttccagcc caatggtccg ccgtttggaa tataccacat ctaccgggat cgttccctct
     2101 ttttctccct ggcgccagcc aacttcgatc ctgcttacca ccttatcacc tcattcctgt
     2161 cctgcaaaca taacatatca caaaaacgca tatccctctg caaaatttga tttttaaaaa
     2221 tacccccggc acaaaatgaa tataatttct ttttgtactg agggtataat cttccttttt
     2281 ctgcctgtca ccaggcacat tttccgctta cagcctacaa cacatctgaa aaatgcggcc
     2341 gcagcttctt aaacatctta agtcccactc caaacaccaa aagagtgaag acccagtaat
     2401 aaatggtcag tttcggtctt tcccagaacc agttgcctgt gatcatactg tccttataac
     2461 cggttacaat gtaataaaat ggattgatct taaataatgg ttcgatccat ggtaagcggt
     2521 tggtaaacat ggactcatga tacatgatcg gagccatcca gataccgaac tgaaggcaga
     2581 tacctacgat ctgggacata tccttgaaaa atacatttac agaggatgta aaatatccaa
     2641 gtcccagtgc cagcatggac gccgcaaagc tgtagtagat cacctggatc caggacagca
     2701 taggcattct tcctgccacc agatacatga tcatcataat gaggacgaaa aatccatgta
     2761 ccagcagaca ggagatcagc ttgatcatag gcaatatctc tactttaaag actacctttt
     2821 tcaccagata actgtattcc tgaagacacc ccgtgatccc gtttaaggct tctccgaaga
     2881 aaaaccatgg aacaataccc gatatcagcc agatcacata gctgactccc ggaacaggcg
     2941 gcggtgattt aaagccgata ccaaagataa ctgcatagat cacaatggta actaccggct
     3001 ggatgaacat ccacacaata ccgaaatagg agcctacaaa acgcttgcga aaatctgctt
     3061 ttcccagatc ccataccagt tttctttttt taatgatctc ttttaaaaga gacaatgcat
     3121 aattcataaa ttcgtttcct tatacttttt tcccggcaga actgtcttag aacttaaaag
     3181 tatcctttaa accgctccac tttttgtcct tatcggagat gatcagctcc atacctggtt
     3241 cgatcggcca gtcaacaccg atctcaggat cgctccataa cagaccgccc tcatcgcctg
     3301 gatgatagaa atcagtacac ttataagcaa actcagcctc atcagataat actaagaagc
     3361 catgtgcaaa accttccgga atataaaact gctttttatt ctcagcagat aaggttacac
     3421 caaaccactt tccataggtc ttggaatcgg aacgcaggtc tactgctaca tcaaatacag
     3481 ttccgcggac agcacgtacc agcttgccct gtggatactg cttctggaaa tgaagtcctc
     3541 ttaaaacgcc ctttacagac atagactggt tgtcctgaac aaaagtcatg ttcagaccag
     3601 cttccttaaa gtcattctgg ttgtaagttt ccatgaagta gcctctttcg tctttgaa
//
LOCUS       DBMDUA010000243         3641 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9018_length_3641_cov_2.130508, whole genome shotgun sequence.
ACCESSION   DBMDUA010000243 DBMDUA010000000
VERSION     DBMDUA010000243.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9018_length_3641_cov_2.130508"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="ACZPCL_12720"
     CDS             complement(<1..686)
                     /locus_tag="ACZPCL_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HIR1163366.1"
                     /translation="MYMKNLEQELKENAYPGRGIVIGESRDGKTAVTAYFIMGRSSNS
                     RNRVFVEDGNGIRTQAFDPAKLEDPSLIIYAPVRVLGNKTIVTNGDQTDTIYEGMDHQ
                     LTFEQSLRSREFEPDAPNYTPRISGVLHVENQKYSYAMSILKSNQGNPDSCLRFTFAY
                     ENPVPGMGHFIHTYMHDGNPLPSFEGEPKPVEIEGNIDEFTDKVWNSLNEDNKVSLFV
                     RFIDIETGKY"
     regulatory      complement(798..878)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(962..2350)
                     /locus_tag="ACZPCL_12725"
     CDS             complement(962..2350)
                     /locus_tag="ACZPCL_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1163367.1"
                     /translation="MRFPDLLMMSVNNLRRRKLRTFLTVLGVIIGTASIVVMVSLGIG
                     LNEMTMEQIASWGSLTTVEVYANDNGNSVRILGGSGSSSSSKSSEPSYITDDVIDEFS
                     RIPYVTGVSPVLEMNVLMRQGAYEAQYISLTGVSQSYLKQLNLGEGRIPAPGEMGMVF
                     GNGVLQRFTNAKTGKGYWDTGELPDVDLMGKPIFVVFDMDAYYQSQGGGTGDDGNSVK
                     PPKKYMIPVTGLIAGGIDDWNNYSWSVYTDIDGLKEQLKKAFKKGTVIPGQPTNKKGK
                     PLNYIVYNSAEVFVDDMEHVAQVQKILADKGYQVNSQMDWLESSRQQSNMVQAVLGGI
                     GAVSLFVAAIGIANTMMMSIYERTKEIGVMKVLGCDMGNIRSMFLIESGFIGFMGGVL
                     GALLSYGVSIIVNRFVSMSDMMGMSGNLSRIPLWLAFAAIGFAIFVGMAAGFMPAMRA
                     MKLSPLAAIRNE"
     gene            complement(2337..>3641)
                     /locus_tag="ACZPCL_12730"
     CDS             complement(2337..>3641)
                     /locus_tag="ACZPCL_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COG1361 S-layer family protein"
                     /protein_id="HIR1163368.1"
                     /translation="KEGNYFVVGENQSTPRGVYPNVLDFDVNFRNKRETTAQDITISM
                     GLSEDNTKFPFEINDGNYDRTYDRIQPGESVSAHYSMAIRKDSYTGYYPIKFTITFRL
                     SSEGDLHTEENTFFVHVVSKDKEDDLRDFDANDRTKARVIVDSYRTEPADVYAGEEFD
                     LILSMKNASSAVPASNILFNLESEKVSDSAVFTTESGTASKVVNSLAPGESTEVRARF
                     TAKAGVDQRSYGITVKEKYDSPEFKNAEESIIVDIPVKQYARLSTSNMDVMPDSMTVG
                     SESNVMFGINNTGKVVLYNVTVNFEADSIKPTDYYVGNIKPGETGNVDTMLSGIAATA
                     DDGTVHVIINYEDENGQPAEPVEKELTLLVEEEVQEDWNMDVPEDMDVSGQPASGANN
                     KLLLAGGGVAFVAVIAAIVFAVKFIKKRKEAKQQKDDENEIS"
BASE COUNT          933 a          908 c          726 g         1074 t
ORIGIN      
        1 tcatattttc cggtttcaat atcaataaaa cggacaaaca gagatacctt attgtcctcg
       61 tttaaactgt tccatacctt atctgtaaat tcatcaatat tgccctcgat ctctacaggc
      121 tttggctcac cttcaaaact tggaagggga ttgccgtcgt gcatataagt atggatgaaa
      181 tggcccattc caggaaccgg attctcatat gcgaaggtaa aacgcaggca ggagtcagga
      241 tttccctggt tgctctttaa aatggacatg gcatagctgt atttctgatt ttccacatgg
      301 agaacgccgg aaatacgtgg tgtgtagttt ggtgcatccg gttcaaattc acggctgcgc
      361 agggactgct caaaggtaag ctgatgatcc attccctcat aaatggtatc cgtctggtca
      421 ccgtttgtaa caatggtctt atttcccagt acacgcaccg gagcgtagat gatcaggctt
      481 ggatcctcta actttgcagg atcaaatgcc tgggtgcgga tcccgtttcc atcttctaca
      541 aatacacggt tgcggctgtt ggagctgcgt cccatgataa aataagcggt aactgctgtc
      601 ttgccgtccc tgctttcgcc aatgacaatt ccacggcccg ggtatgcatt ctcttttaat
      661 tcctgttcca gatttttcat atacatggta tgatcctcct gatttctata aggtgtaagg
      721 tgtatgtatt cccgccaaag caggaaacag atcagctata gcattcaaaa aaccgcccac
      781 ctgcacacga ataacctatt tcgtgcccag gcggacggct ctcacttcct gcgtgctccc
      841 tgtggttgat ccactttccg ccagtcacac gctgtgcttt ctaactgttt ttaatatata
      901 tggtttttac taataatgca agtgcttttt agaagtttta cttattagtt tttcttataa
      961 tttattcatt gcggatcgca gctaaggggc ttaatttcat agcacgcata gcaggcatga
     1021 aaccggctgc cattcccaca aaaatagcaa agcctatagc tgcaaatgcc agccataaag
     1081 ggatcctgga taaatttccg ctcatcccca tcatatcaga catgcttaca aaacggttga
     1141 cgataatgga cacaccatag cttaacagtg ctcccagcac gccgcccata aaaccgatga
     1201 aaccggactc gatcaaaaac atactgcgga tatttcccat atcacagcct aaaaccttca
     1261 taacaccgat ctctttggtc ctctcataaa tggacatcat catggtgttt gcaataccga
     1321 tagctgctac aaacagagac acagctccaa tgcccccaag aactgcctgt accatattgg
     1381 actgctgtct ggagctttcc agccagtcca tctggctgtt gacctgatag cccttatctg
     1441 ccaggatctt ctgcacctgg gccacatgtt ccatatcatc tacaaagact tctgcactgt
     1501 tgtatacaat ataatttaat ggctttcctt ttttattcgt aggctgtccc gggatcacgg
     1561 ttcccttttt aaaagccttt ttcagctgtt cttttaagcc gtcaatatct gtatatacag
     1621 accaggaata attattccag tcatcgatgc cgcctgcgat cagtcctgtt acagggatca
     1681 tatatttctt cggcggtttc acactgtttc catcatctcc cgtgccgcct ccctggcttt
     1741 gataataagc atccatatca aaaaccacaa agatcggctt tcccataaga tccacatccg
     1801 gcagttctcc cgtatcccag tagccttttc ctgttttcgc gttggtaaaa cgctggagaa
     1861 caccatttcc aaagaccatt cccatttcac cgggtgccgg gatacggcct tctcctaaat
     1921 tcagctgctt taaataggac tggctgacac cggtaaggct gatatactga gcctcataag
     1981 ccccctggcg catcagcaca ttcatttcca ggaccggaga tacacctgtt acatagggga
     2041 tcctggaaaa ctcatcaatc acatcatccg taatataaga tggctcactg cttttagatg
     2101 aggatgaaga gccggaacct cccaggatcc ttacagagtt tccattatca tttgcataca
     2161 cctctacggt agtcagactg ccccaggaag cgatctgttc catggtcatc tcatttaaac
     2221 caattcccag ggaaaccatg actacgatcg aagccgttcc tataataaca cctaaaactg
     2281 taagaaatgt ccttaatttt cgtctgcgca ggttattgac gctcatcatc agaagatcag
     2341 gaaatctcat tttcatcatc cttttgctgt tttgcttctt tgcgcttttt gatgaacttc
     2401 actgcaaata cgattgctgc aatgacagcc acaaaagcca caccgcctcc cgctaaaaga
     2461 agcttattgt ttgccccgga agcaggctgt ccggaaacat ccatatcttc cggcacatcc
     2521 atattccagt cttcctggac ctcttcctct accagaagag tcagctcttt ttccaccggc
     2581 tctgcaggct gtccattttc atcttcatag ttgatgatca catggactgt accatcatct
     2641 gccgttgcgg cgataccgga aagcatggta tctacatttc ctgtttcacc tggtttgata
     2701 tttcctacat aataatcggt cggtttaatg gagtcagctt caaaattgac agtcacatta
     2761 taaaggacta cttttcctgt attgttaatg ccgaacataa cattggactc agaacctacg
     2821 gtcatggagt caggcattac atccatattg ctggtgctca gtcttgcata ctgctttacc
     2881 gggatatcca cgataatgct ctcttctgcg ttcttaaatt ccgggctgtc atatttttct
     2941 tttactgtaa tgccatagga acgctggtca acacctgctt tggctgtaaa acgggcccgg
     3001 acctctgtac tttctcctgg tgccagggaa ttgactactt tggaagcagt tccgctttct
     3061 gtggtaaata cagcgctgtc agaaaccttt tctgactcca gattaaataa aatattgctg
     3121 gcaggtactg cagaggaggc atttttcatg cttaaaataa ggtcgaattc ttctcctgca
     3181 tacacatccg ccggttctgt gcggtagctg tctacgatca cacgggcttt ggtacggtca
     3241 ttggcatcaa agtcacggag atcatcttct ttgtccttgg ataccacatg gacgaaaaat
     3301 gtattttctt ctgtgtgaag atcaccttca gaacttaagc ggaaagtaat ggtaaattta
     3361 ataggataat acccggtata agagtctttt cggatagcca tgctgtagtg ggcagaaacg
     3421 ctttctcccg gctggatccg gtcgtaggtg cggtcatagt tgccatcgtt gatttcaaag
     3481 gggaacttgg tattatcttc cgataagccc atggatatag tgatatcctg ggcggtagtt
     3541 tcacgcttgt tgcggaaatt tacatcaaaa tcaaggacat tgggataaac acctcttgga
     3601 gtagactggt tttcgcctac tacaaaataa tttccctctt t
//
LOCUS       DBMDUA010000244         3637 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9032_length_3637_cov_1.582296, whole genome shotgun sequence.
ACCESSION   DBMDUA010000244 DBMDUA010000000
VERSION     DBMDUA010000244.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9032_length_3637_cov_1.582296"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(181..1575)
                     /locus_tag="ACZPCL_12735"
     CDS             complement(181..1575)
                     /locus_tag="ACZPCL_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HIR1163369.1"
                     /translation="MEKTKEKRSFQAPHTFVILVVLILIAVAATYVVPAGEYTRYADE
                     ATGRTLVEAGSFHYVESAPVSFFSIPSLIYRAIVKAASTVTFILIIGGSFEIITSTGV
                     LTVLCKKMSKAFKGREFFVIPAFLVLFSIFGATMGMSTEVMIFVPIGIALATSLGLDK
                     VTGTAMIAMGAASGFTAGILNPFNVGVAQDIAEIPMFSGMGYRLFILVVLLVINTVYI
                     TWYAKRVKADPAKSIIYGEPDDNEFVFDDKDEAMTLTHGLTLGVIVVGFAALIYGLSK
                     LDWYFEEMSAIFITMGVVCGIINRYSPNKIASTFGAGAKGIVVGALIVGIARGVEMAL
                     SDALILDTIVHAIASLVNTMPQSLKAVGMFLAQSLVNCVITSGTGQAAVTMPLMVPVA
                     DLIGITRQTAVLAFQLGDGFSNSVLPTSSALMGYLIVSKIPYAKWLKFMMPLFLIWTF
                     AGCLFMLGAIGIGY"
     gene            1723..2634
                     /locus_tag="ACZPCL_12740"
     CDS             1723..2634
                     /locus_tag="ACZPCL_12740"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1163370.1"
                     /translation="MDMKEQVYMLAIEKYKGIKDAAQSLHISPPALSIFLSNLEERSG
                     VHFFDRIGKQFIPTQAGKIYLEHARKMINIRDDYEDELKKYIHGSSGTIRFGIHPRRT
                     LYLLSQVLPRFSARYPDVELIPYEASSERMVELLLNGELDFAIINLTSDHPLLEYHLL
                     YEDYMVAVVAADHPVCKKMGPASTDDDPVLIDLKKFNGERFILQKSEQSSRHYTDLAI
                     AHDHAEPGSIFLLENLESSAQLAAEGYGISFNFYQYMRRFHYPKPVRCFRIGGSAEKT
                     IYSIVTLKGKHTIAPMQELIELIKQFA"
     gene            complement(2754..>3637)
                     /locus_tag="ACZPCL_12745"
     CDS             complement(2754..>3637)
                     /locus_tag="ACZPCL_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIR1163371.1"
                     /translation="YSFKASVQNGTFSTEGITVLLALIGILITAVLLVKQVKGGILWG
                     ILATWILGIICQLAGIYQVNPEAGAYSLLPDFSNGIAIPSMAPTLMKMDFSSMFSLDF
                     FAVVFAFLFVDLFDTLGTLIGVASKADMLDKDGKLPRIRGALLSDAVGTTVGAMFGTS
                     TVTTFVESASGVAEGGRTGLTAAVAAILFGLSLFLSPIFLAIPSFATAPALIVVGFLM
                     ISSILKIDFNDYTESIPAYIGIIAMPFMYSISEGISMGIISYVVINLVCGKAKEKKIS
                     PLLYILAVLFILKYIML"
BASE COUNT         1108 a          803 c          728 g          998 t
ORIGIN      
        1 gactccgtat ttttttaata cgtctgaaat ataggcaact gcctgaagtg cttccggttc
       61 tcctgtcagt ttatcaaagg aacacaaata ttccagatgt ttttttaacc gttctttctc
      121 ttccattttt attttccctc ctgttaccgt tatctggttc cgttatttcc caactgttta
      181 ttaataacca attccaatgg cgcccagcat aaacaggcag cctgcaaatg tccagatcaa
      241 aaacaaaggc atcataaatt tcagccactt tgcataaggg atcttggaaa cgatcagata
      301 tcccattaat gcagaggaag ttggcagaac agagttggaa aatccatcac ctaactggaa
      361 tgcaagaaca gctgtctgtc tggtaattcc gatcagatct gctacaggta ccattaacgg
      421 catggtaaca gctgcctgtc cggtaccgga tgtgatgacg cagtttacca gtgactgtgc
      481 taaaaacatt cccacagcct ttaaggactg aggcattgta tttacaagac ttgcaatggc
      541 atgaacgatg gtatctaaga tcagggcatc tgaaagtgcc atttccacgc cgcgggcaat
      601 accgacgatc agggcgccta ctacaattcc cttcgctccg gccccaaagg tagaagcgat
      661 cttattcgga ctgtaacggt taataatgcc gcagacaact cccatggtaa taaagatcgc
      721 tgacatttct tcaaaatacc agtccagttt acttaaacca tagatcaggg cggcaaaacc
      781 taccacgatc actcccagtg taagaccgtg tgtcagagtc attgcctcat ctttatcatc
      841 aaaaacaaat tcattgtcat ctggttcccc atagataatg ctcttggcag ggtctgcttt
      901 tacacgtttt gcataccagg taatataaac cgtattaatg accagcaata ctacaaggat
      961 aaataatctg tatcccatac cggaaaacat ggggatttcc gcaatatcct gggcaacacc
     1021 aacattaaat ggatttaaga taccagcagt aaaaccggaa gccgcaccca tagcgatcat
     1081 agctgtacct gttactttat ccagtcccag tgaagtggcc agagcaattc cgatcggtac
     1141 aaagatcata acttccgttg acattcccat ggttgcgcca aaaatggaaa acagtaccag
     1201 aaaagccggg ataacaaaaa attctcttcc cttaaatgct tttgacattt ttttgcataa
     1261 aacagtcagc acccctgtgg aggtaatgat ctcaaaagaa cctccgatga tcaggataaa
     1321 ggttactgta cttgctgctt taacgatcgc ccggtagatc aatgaaggga ttgaaaaaaa
     1381 gcttaccggg gctgactcta catagtggaa actgcctgct tcaacaaggg ttcttcctgt
     1441 tgcctcatct gcataccggg tatactctcc tgccggtaca acataggttg ctgctactgc
     1501 gatcaggatc agcactacaa ggataacaaa tgtatgcgga gcctgaaacg accttttttc
     1561 ttttgtcttt tccataattc atgccctttc ttgataattt ttatttctta aactataatg
     1621 aataaataaa attaaatcaa gtttaatatt tttaatattt catttaatat tatttatttt
     1681 tatgcatagt atacaaatat ttatacattg gagggaaaaa ctatggatat gaaggaacag
     1741 gtttacatgc ttgctattga aaaatataaa ggaataaaag atgcagccca gtctctgcat
     1801 atttccccgc ctgcactgag tattttttta agtaatctgg aagaacgcag cggtgtgcat
     1861 ttttttgacc ggatcgggaa acagtttata cctacccagg cgggaaaaat ttatctggaa
     1921 catgcaagaa aaatgataaa catacgagat gattatgaag acgaattaaa gaaatatata
     1981 cacggttcct ctggaacgat ccgctttggg atccacccaa gacggactct ttatcttcta
     2041 tcccaggttc tgccacgttt ctctgccagg tatccggatg tggagctgat cccttatgaa
     2101 gcttcttcgg agcgtatggt agaacttctc ttaaatggag aactggactt tgccattatc
     2161 aatctcacct cggatcatcc gcttttagaa taccacttat tatatgagga ctatatggtt
     2221 gcggttgtag ctgccgatca cccggtctgt aaaaaaatgg gacccgcttc cactgatgat
     2281 gatcctgtat tgatcgatct gaaaaaattt aacggtgaac gttttattct ccagaaatcg
     2341 gagcagtctt ccagacatta tacagatctg gcaattgccc atgatcacgc agaacccgga
     2401 agcatttttc ttttggaaaa tctggagtct tctgcccagt tagcagcaga gggttatgga
     2461 atttccttta atttttacca gtatatgaga cgtttccatt atccaaaacc agtacgctgt
     2521 tttcggatcg gaggttccgc tgaaaaaacc atatattcta ttgttacatt gaaaggaaaa
     2581 cataccattg ccccgatgca ggaactgatc gaactcatca agcagtttgc ttaaaatcgt
     2641 taagcagatg gatcaggtat aaactaccaa aaaggagccg ccagggggcg actccttttg
     2701 atatgcatgt gcagcaacca ttcggttgtc aataatccgc aaaaacggat caactacagc
     2761 ataatatact tcaatataaa taatactgcc agaatataaa gcagtgggct gatcttcttc
     2821 tcttttgcct taccgcatac caggttgatc actacatagg agataattcc catagagata
     2881 ccttcggaaa tgctgtacat gaaaggcatt gcgataatac cgatatatgc aggaatggac
     2941 tcggtatagt cattaaagtc aatcttcagg atagaggaga tcattaaaaa tcctactacg
     3001 atcagcgccg gtgcagttgc aaaggacggg atcgcaagga agattggtga taagaacaga
     3061 gacagtccaa acaggattgc tgctactgca gcggtcagtc cggttcttcc gccttctgct
     3121 acaccagaag cactttctac gaaagtagtt actgtagaag taccaaacat agcacctaca
     3181 gtagtaccaa ctgcatcaga taaaagggca ccgcggatcc ttggaagctt tccgtctttg
     3241 tccaacatgt ctgctttgga agcaacaccg atcagtgttc ccagtgtatc aaacaggtct
     3301 acaaacagga atgcaaatac aactgcaaag aaatccagtg agaacatgct gctgaaatcc
     3361 atcttcatca gggttggtgc catacttggg atagcaatac cattggaaaa atctgggagc
     3421 agactatagg ctcctgcttc tggatttacc tgatagatac ctgctaactg gcagatgata
     3481 cctaagatcc aggttgcaag gataccccag aggataccgc cttttacctg ttttaccagt
     3541 aaaacagctg tgatcaggat accgatcagt gctaacagaa cagtgatgcc ctctgtggag
     3601 aatgtaccat tctgaacaga tgccttaaag gaataaa
//
LOCUS       DBMDUA010000245         3615 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9097_length_3615_cov_2.326978, whole genome shotgun sequence.
ACCESSION   DBMDUA010000245 DBMDUA010000000
VERSION     DBMDUA010000245.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9097_length_3615_cov_2.326978"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            291..2300
                     /gene="htpG"
                     /locus_tag="ACZPCL_12750"
     CDS             291..2300
                     /gene="htpG"
                     /locus_tag="ACZPCL_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIR1163372.1"
                     /translation="MAKKGSLSITSENIFPVIKKWLYSDHDIFYRELISNGCDAITKL
                     KKLELMGEYEKPEEVEYKIQVSVNPTDKTITIEDNGLGMTEEEIDKYINQIAFSGVQD
                     FMEKYKDKANEDQIIGHFGLGFYSAFMVADKVEINSLSYQKDAKPVHWESEGGINFEM
                     AEGDKKEVGTSIKLYLNEESTEFANEYRAREVIEKYCAFMPVNIFLKNETAEPEYETI
                     EKDELTHKDTVVETVIEPAKTEEKEKEDGTKETVEVEPSREKYKILKRPVALNDTNPL
                     WNKHPNECTDEQYKDFYRKVFRDYKEPLFWIHLNMDYPFNLKGILYFPKINMEYDSLE
                     GTIKLYNNQVFIADNIKEVIPEFLMLLKGVIDCPDLPLNVSRSALQNDGFVKKISDYI
                     TKKVADKLSGMCKTDRENYEKYWDDIAPFIKYGYIKDEKFAEKMGDYILYKNLEGKYL
                     TLNDCLEENKEKHENTIFYVTNEKEQSQYINMFKEEGIDAVIMPAAIDNPFISHVEQK
                     HEGLKFLRIDTDLNASFKEEVKEDDEAFKKTSEELTEVFKKALNNDKLDIKVEKMKNA
                     SVASMITVSEETRRMQDMMKMYSMGGMDMSAFGATGETLVLNANHPLVKYVLEHKDGE
                     NTAKICEQLYDLASLAHGQLSPERMTGFVNRSNDIMMIMAGQKAE"
     gene            2464..3591
                     /locus_tag="ACZPCL_12755"
     CDS             2464..3591
                     /locus_tag="ACZPCL_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163373.1"
                     /translation="MKKRTLSRKVLTLALGMSLAAAGAFAAFADPLGLSYINGQTVTS
                     RMGGFEFSAPAEYKVDRSGASSALSGEIVAGLVEDSGKIDFAASMVDETNKSYIINTA
                     MVLPADDGSGQPFSVETLKAELAESGLDPADTESGTVKLGAADYEYLKVNYGKMMADS
                     MRGQLESGQLSEKQKEQAKALLDRMEKLLVNDFYIRNTGDKIYVLCQTYSVDQAERAA
                     AFLGLLKPYTGVEGWYQNGENWQYKKADGQLALNCWEQDENGLTYHLDGNGNIEYNAW
                     VQENGGWKYADESGHMVTSVTKTINGVQYTFDDKGNMIAGSEKAAPDYSLGKLEGNTY
                     TNYWADMTLSFPEEATVMIGNGSAQTYACFIGSIACKATFN"
BASE COUNT         1168 a          657 c          911 g          879 t
ORIGIN      
        1 taaaagacca cctctaaatg ggatattgta ggttcacaca ccaccggcca tcaggctttt
       61 ttcctgtttt tgcaggtgat ctgttcgcta gtacatcgtt tcgtaaataa ctgtactaat
      121 cacgtaggat gcggatttga gtgtgcaggt ctaaaaacgc aggcgtagcg ggctacgctg
      181 aggcttttag gcctgtgcaa tcagattcgc agacaagtga gtggtatagt tattttgaaa
      241 acgatgtact agaaaagatt aagaagattt ataagaaaga ggcgtatatt atggcaaaga
      301 aaggcagttt atctataaca agtgaaaata ttttccctgt aatcaaaaag tggctgtatt
      361 ccgaccatga cattttttac agagagctga tcagcaatgg ctgcgatgct attactaaat
      421 taaagaaatt agagctgatg ggagagtacg aaaagccaga agaggttgag tacaagatcc
      481 aggtcagcgt aaatcctaca gataagacca tcaccattga agataatggt cttggtatga
      541 ctgaggaaga gatcgacaag tatattaacc agatcgcatt ctccggtgtt caggatttta
      601 tggaaaagta caaagataag gcaaatgaag accagatcat cggccacttt ggtcttggtt
      661 tctactctgc atttatggta gctgacaagg ttgagatcaa ttccctgtct taccagaagg
      721 atgcaaagcc ggtacactgg gagtctgaag gcggcatcaa ctttgagatg gccgaaggcg
      781 acaaaaagga ggttggtact tccattaagc tgtatctgaa cgaagaaagc actgaatttg
      841 caaatgaata cagagcaaga gaagtgatcg aaaaatactg tgcatttatg ccggtaaaca
      901 ttttcttaaa gaatgagact gcagagccgg aatatgagac catcgaaaaa gatgagctta
      961 ctcataagga tacagttgtt gagacagtta ttgagcctgc aaagacagaa gagaaggaaa
     1021 aagaggatgg aaccaaggaa acagtagaag ttgagccatc cagagagaaa tataagatct
     1081 taaagcgccc ggttgcatta aacgatacca acccgctgtg gaacaagcat ccaaatgagt
     1141 gcaccgacga gcagtacaag gacttctacc gcaaggtatt cagggattac aaggaaccgc
     1201 tgttctggat ccatctgaac atggattatc catttaactt aaagggtatc ttatacttcc
     1261 cgaagatcaa catggaatac gacagcttag agggaactat caagctgtac aacaaccagg
     1321 tatttatcgc tgataacatt aaggaagtca tccctgagtt cctgatgttg ttaaagggcg
     1381 ttattgattg tcctgatctg cctcttaacg tgtcacgtag cgcgctgcag aatgacggtt
     1441 ttgtaaagaa gatctctgat tatattacca agaaggtagc agacaagctg tccggtatgt
     1501 gcaagactga ccgtgagaac tatgagaaat actgggacga tattgctcca tttatcaaat
     1561 atggttatat caaggacgag aagtttgctg agaagatggg cgactatatc ctgtataaga
     1621 acctggaagg caagtatctt accttaaatg actgcctgga agagaacaag gaaaaacatg
     1681 aaaataccat tttctatgta accaacgaaa aagagcagag ccagtatatc aacatgttta
     1741 aggaagaagg cattgacgca gtgatcatgc ctgccgctat tgataatcca tttatcagcc
     1801 atgtagagca gaagcatgaa ggacttaagt tcctgcgtat tgatacagat ttaaatgctt
     1861 ctttcaagga agaagtaaag gaagatgacg aagcctttaa gaagaccagc gaagagctga
     1921 cagaggtatt taagaaggct ttaaataacg ataagctgga tatcaaggtt gagaagatga
     1981 agaacgcatc tgttgcatct atgatcactg tatctgagga aacaagacgt atgcaggata
     2041 tgatgaagat gtacagcatg ggcggtatgg atatgagtgc tttcggcgca acaggcgaga
     2101 ctcttgtatt aaatgctaat catccactgg taaaatatgt tctggagcac aaagacgggg
     2161 aaaatacagc caagatctgc gaacagctgt atgatctggc aagcctggca catggccagc
     2221 tgtcaccgga gcgtatgacc gggtttgtaa accgcagcaa tgatattatg atgatcatgg
     2281 ctggacagaa agcagaataa atagtacaat aagaagcacg acacagagtt gcgggggctg
     2341 tttcacaggt attttgcctg ggtcggcccc tgcttttttg ttagatgtac tcgcaaaatc
     2401 ggatacaaaa agatcctgag agtacatggg ttgaatatat ttgaaagtac agggagaata
     2461 tatatgaaaa aacgtacctt atcaagaaaa gtgctgacac tggctcttgg aatgtcactg
     2521 gcggcagcag gtgcttttgc agcttttgca gatccgctgg gcttaagcta tattaatggt
     2581 cagactgtaa caagccgtat gggcggcttt gaatttagcg caccggcaga gtataaagta
     2641 gacagaagtg gcgcttcttc ggctctttcc ggggagatcg tagcgggtct tgtggaagat
     2701 agcggaaaaa tcgattttgc ggcgtccatg gtagatgaaa ccaataaaag ttatatcatc
     2761 aatacagcca tggtcctgcc tgcagatgat ggttccggac agccttttag cgtagaaacc
     2821 ttaaaagcgg agctggcaga gtccggtctg gatccggcag atacagaaag cggtacagta
     2881 aagctgggag ctgcagatta tgagtatctg aaggtaaatt acggaaaaat gatggcagac
     2941 tccatgaggg gacaactgga gtcaggacag ctttctgaaa aacagaaaga gcaggcaaag
     3001 gcattattag accggatgga aaaactgcta gtcaatgatt tttatatccg gaatacagga
     3061 gacaagatct atgtgctctg ccagacctat tctgttgacc aggcagagcg tgcagcagcc
     3121 tttttaggtc ttttaaagcc ttatacagga gtagaaggct ggtaccagaa cggggaaaac
     3181 tggcagtata aaaaggcaga cggacagttg gcgttaaact gctgggaaca ggatgaaaat
     3241 ggccttacct atcatctgga cggaaacgga aatattgagt acaatgcctg ggtacaggaa
     3301 aatggcggct ggaagtatgc agatgaaagt ggtcatatgg ttacttctgt aacaaagacc
     3361 attaacggag tccagtatac ctttgatgat aagggaaata tgatcgccgg aagtgagaaa
     3421 gcggccccgg attattccct tggaaaactg gaaggaaata cctatacgaa ttactgggca
     3481 gatatgacac tttccttccc ggaggaagct actgttatga tcggaaatgg cagtgcccag
     3541 acctatgcct gttttatcgg ctcaatagct tgtaaagcta ccttcaactg aacgacttca
     3601 cggggcgaca cacgt
//
LOCUS       DBMDUA010000246         3570 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9217_length_3570_cov_3.113001, whole genome shotgun sequence.
ACCESSION   DBMDUA010000246 DBMDUA010000000
VERSION     DBMDUA010000246.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9217_length_3570_cov_3.113001"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="ACZPCL_12760"
     CDS             <1..1087
                     /locus_tag="ACZPCL_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR1163374.1"
                     /translation="CVLNWLSESILGISSELRQMALIIILLKAGLSLNLADLKKVGRP
                     ALMMSCVPASFEILAFVIFAPAILHISRVEAAVMGAVLGAVSPAVVIPRMVQLMETKY
                     GTDQRIPQMIMAGASCDDIFVIVLFSTFTNMAQGGSAHVADFINIPVSILLGIALGAV
                     AGYGLSLFFETAYAKEHYVRNSMKVILILGMSFFLMSVETWLKGKVSVSGLLAVVSMA
                     AVIRIKCIPKVSKRLSEKFGKLWIAAEVILFVLVGAAVDIRYTMQAGIAAVLMIFVGL
                     MFRAVGVSLCMLGTKLNKKERLFCVIAYLPKATVQAAIGSVPLAMGLPCGQIVLSVAV
                     LAILITAPLGAAGMDLTYDKLLVRSED"
     gene            complement(1054..1974)
                     /locus_tag="ACZPCL_12765"
     CDS             complement(1054..1974)
                     /locus_tag="ACZPCL_12765"
                     /inference="COORDINATES: protein motif:HMM:NF014376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1163375.1"
                     /translation="MNVSFPKCNQAGTEFVISTTDDLQEKVNYLSEDFPVAIFTQTYH
                     HAASDSTPFHWHNELQLNWVSEGELEYCINGDSLRLSGDKILLVKDHQLHSSRTTEKD
                     TCSLCINFSPDIFHPMILKHYIRPFLENENFTSSIQLLNPGQINILESLLDQTSEPLG
                     YFSIVNFLSQVFEELIRSFDEKPDQNDKEEKELFQSMLSFVHSHYQDPLTVRQIAAYG
                     IINKNRCTDLFRKYTRLSPIKYLNEYRLYMAKNLILNTKKPISEISADVGYNQISHFI
                     EQFRGAYGLSPLKYRNQFAGQSSDRTSSLS"
     gene            2214..3461
                     /locus_tag="ACZPCL_12770"
     CDS             2214..3461
                     /locus_tag="ACZPCL_12770"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HIR1163376.1"
                     /translation="MISVNGERLLARINELGAIGKDAEGRRTRLAASDTEKQGRDLVS
                     KWMKEAGLEVVVDRIGNIFGIWETPENKDQAPLMIGSHIDTVINAGQYDGCLGVISSI
                     EVIKTLKDAGMVSARPIVAAAFTNEEGVRYSPDMMGSLVYAGGMDVEDALDAVGTDGT
                     VLRDELKRIGYEGTVEPGFIKPYAFVELHIEQGPILDHEGIRIGAVENLQGIHWQRVT
                     IEGAANHAGTTPTALRHDAGLAAAKVNVFLRQLVEKSGGVATVGCIEFKPNAINVIPS
                     KAVFTVDLRNPDKEKLDNDEKILAAYLKDLAKEDDVKISTEHLTEFDPVPFDEGIVEK
                     VEKAAKAHGLSVRRITFGAGQDAQMIARICPTAMIFVPSVKGISHNPEEYTKDEDVLA
                     GAGVFLDVVMDMAVVGVPAARNN"
BASE COUNT         1004 a          669 c          925 g          972 t
ORIGIN      
        1 ctgtgtatta aactggcttt cagagtcgat cctggggatc tcttcggaat tgcggcagat
       61 ggcattgatc atcatactgt taaaagcggg attgtccctg aatttggcag atctaaagaa
      121 agtaggcaga ccggctctta tgatgtcctg tgttcctgcc agttttgaaa ttctggcctt
      181 tgttattttt gccccggcta tcctgcatat ttcaagagtg gaagcggctg taatgggagc
      241 tgttttaggc gcagtgtcac cggcagttgt gatcccgcga atggttcagc tgatggagac
      301 aaaatatgga acagaccaga gaattccgca gatgatcatg gccggtgcat cctgtgatga
      361 tatttttgtg atcgtcctgt tttccacatt caccaatatg gctcagggcg gcagtgcaca
      421 tgttgcagat tttatcaata ttccggtatc cattctttta ggcatagcac tgggagcagt
      481 ggcaggatac gggctcagct tgttctttga aacagcttat gcaaaagaac attatgtgcg
      541 caacagtatg aaagtaattc tgatcctggg aatgtcattt ttcttaatgt ctgtggaaac
      601 atggttaaag ggaaaggtat ctgtttccgg tctgctggca gttgtaagta tggcagcagt
      661 gatccggatc aaatgtattc ccaaggtatc aaaacgtctt tcagagaaat ttggaaaact
      721 gtggattgca gcagaggtga tcctttttgt actggtaggt gcagctgtgg atatccgtta
      781 taccatgcag gcagggatcg cagcagtgct gatgatcttt gtgggcttaa tgttccgggc
      841 tgtgggagta tccctttgta tgctgggcac gaaattaaac aaaaaagagc ggctgttttg
      901 tgtgatcgcc tatcttccaa aagcaacggt acaggcagcg atcggatccg tacctcttgc
      961 aatgggattg ccctgtggcc agatcgttct gtcagtagcg gttttagcaa tcctgatcac
     1021 agcgccttta ggagcagcag gaatggatct gacttatgat aaactgctgg tacgatcaga
     1081 agactgacca gcaaactggt tcctgtattt taagggagaa aggccatagg caccccggaa
     1141 ctgttcaata aaatggctga tctgattgta tcctacgtca gcactgatct cagatatagg
     1201 ttttttcgtg tttaggatca gattttttgc catataaagc cggtattcgt tcaggtattt
     1261 aataggggac agccgggtat atttacggaa caggtcggta cagcgattct tattgatgat
     1321 cccgtaggca gcgatctggc ggactgtcaa aggatcctga taatggctgt ggacgaaact
     1381 taacatggac tggaacaatt ctttttcttc tttatcgttc tggtccggtt tttcatcaaa
     1441 ggagcggatc agttcctcaa atacctggga tagaaaattt acaatggaaa aatatcccaa
     1501 aggttcgctg gtctgatcta aaagactttc taaaatgttg atctggcccg gatttaaaag
     1561 ctggatggaa ctggtgaaat tttcgttttc caggaaaggc cggatatagt gttttaagat
     1621 catgggatgg aaaatgtcag gagagaagtt gatacacagg gaacaggtat ctttttcggt
     1681 ggtgcgggaa ctgtgaagct gatggtcctt taccagaagg atcttatctc cggacaggcg
     1741 gagagagtca ccattgatgc agtattccag ctctccctct gaaacccagt ttaattgcag
     1801 ctcattgtgc cagtggaaag gtgtggagtc actggcagca tggtgatagg tctgtgtgaa
     1861 aatggcaaca ggaaagtctt ctgacaggta attgactttt tcctgcaggt catcggtagt
     1921 ggatatgaca aattcagttc cggcctgatt gcattttgga aaggaaacat tcataaagta
     1981 tcattctcct tttgtatatg tatgtttgta tatttagtat gttcagagtc ttttctgata
     2041 tgaattgtcc aaagtatatc acttttccct gtttttcgga atggggacag tgataatctg
     2101 gtacaaatgt gatataatcc gttctattag tgatagaaac ataaggtcca tgtgactata
     2161 ctgagggtac agaccggaca gaacgctgcc ggataaaatg aggaggtcat attatgatat
     2221 ctgtaaacgg agaaagatta ctggcaagga tcaacgaact gggagcaatt ggaaaggacg
     2281 cagagggaag aagaacccga ctggctgctt ctgatacaga aaaacagggc agggacctgg
     2341 tttccaaatg gatgaaggaa gctggcttag aggtagtagt agaccggatc ggaaatattt
     2401 tcggtatctg ggagactcct gaaaataaag atcaggcgcc ccttatgatc ggttcccata
     2461 ttgatacggt catcaatgct ggacagtatg acggatgtct gggtgtgatc tccagtattg
     2521 aagtgatcaa aactttaaag gatgcaggaa tggtttctgc aaggcctatt gttgcagctg
     2581 cttttaccaa tgaggaaggt gtccgctatt caccagatat gatgggatct ttagtttatg
     2641 ctggcggtat ggatgtagaa gacgctctgg atgcagtagg aacagatgga actgttttaa
     2701 gagatgaatt aaagcgcatc ggatatgaag gaaccgtaga gcctggattt attaaaccat
     2761 atgcatttgt ggaactgcac attgaacagg gaccgattct tgaccatgag ggcattcgca
     2821 tcggcgcagt agaaaacctt cagggcatcc actggcagag agttacgatc gaaggtgctg
     2881 caaaccatgc aggaaccaca ccaactgcat taagacatga cgcaggactg gcagcagcaa
     2941 aggtaaatgt attcttaaga cagttagtgg agaagtcagg gggcgttgct accgtaggct
     3001 gtattgaatt taagccaaat gccatcaacg ttatcccttc aaaggctgta tttactgtgg
     3061 atttaagaaa cccagacaaa gaaaagctgg ataatgacga gaagatcctg gctgcgtatt
     3121 taaaggatct ggcaaaagaa gatgatgtta agatctccac cgagcatctg acagaatttg
     3181 atccggttcc atttgacgaa ggcattgtag aaaaagtgga aaaggcagca aaagcccatg
     3241 gtttaagtgt aagacgcatc actttcggcg cagggcagga tgcccagatg atcgcaagga
     3301 tctgcccgac agctatgatc tttgtaccaa gcgttaaggg catcagccac aacccggaag
     3361 aatataccaa agacgaagac gtactggcag gcgcaggcgt attcttagat gttgtaatgg
     3421 acatggcagt tgtgggagtg ccagcggcac gtaacaactg actttcatta agtccagcgg
     3481 tgtgatgtca agaaaataaa tgatttaaaa atcatacatt tttgatatat accgcaaaga
     3541 caaaaaacac ccacctaagc cctgtcgacc
//
LOCUS       DBMDUA010000247         3563 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9249_length_3563_cov_2.487291, whole genome shotgun sequence.
ACCESSION   DBMDUA010000247 DBMDUA010000000
VERSION     DBMDUA010000247.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9249_length_3563_cov_2.487291"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="ACZPCL_12775"
     CDS             <1..788
                     /locus_tag="ACZPCL_12775"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HIR1163377.1"
                     /translation="LIKREVSDGVIAPGYEPEALEILKSKKNGNYNVIQIDPDYVPEE
                     LERKQVFGVVFEQGHNNLKIDKEMLKNIVTENKDLTEEAAMDLVISLITLKYTQSNSV
                     CFVKGGQAIGIGAGQQSRVHCTRLAGSKADNWYLRQNPKVLNLPFIDGIRRADRDNAI
                     DLYIGEDYMDVLADGRWEKIFKEKPEVFTREEKRAWLDGLTDVALGSDAFFPFGDNID
                     RAHKSGVKYVAQPGGSVRDDQVIDTCNQYGMVMAFTGIRLFHH"
     gene            868..2205
                     /locus_tag="ACZPCL_12780"
     CDS             868..2205
                     /locus_tag="ACZPCL_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1163378.1"
                     /translation="MMKDLTREHPDKILWRFLLPMLVSVMFQQIYNIADSCIAGRFAG
                     EDALAAVGASYPITVIFMAFAVGTNLGASVVVSRLFGAKDMARMKSAVSTAFVACAFM
                     SFFLTLIGYFFCGSMMRAVHTPENIFADGQLYLKIYVYGITFLLLYNVCTGIFTALGD
                     SKTPLYFLIGSSVGNIILDYYFVACVGLGVAGVAWATFIAQGISAILALTFLAMRLKK
                     INCEGTFQWLDLVLLAQIAAIAIPSIMQQSVLSIGNMFVQEIVNRYGSAVIAGYSGAI
                     KLNTFAINSFMALGSCLSSYTAQNLGAGKKERLPLGFRTGIRLSVIASLPFIVLYFFA
                     SRQMMGLFLNDSSIDAINAGQKFLHIVSPMYFMISIKLMTDGIIRGAGAMHYFVMATV
                     PDLILRILVALLLTQYFGSTGIWMAWPFGWIAATVLTIIFYRRIITGKYVIRL"
     gene            2479..>3563
                     /locus_tag="ACZPCL_12785"
     CDS             2479..>3563
                     /locus_tag="ACZPCL_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR1163379.1"
                     /translation="MIKGTGYLCLFLVPCLSYYLFEYVTGNLENISIFMAALNICWAY
                     VLYLVLTGITGTTRISVPVVSIALLILSFAETFVVDFRDRPIMIWDVLAVRTAMTVSG
                     NYEFDVTKQMIQAAKAVIGANMILWFFPVHVKGLKKRLVFGGTCIGTAAAFVFGFFHS
                     VVPAHQMGINMWAVNDTYDSCGYILSTAMSLQYVVKKPPVEYSHGKLEAIYKELTEKE
                     EQEKEAKAEGTQKTGAGEETVQPVNLICIMNESLSDLRVVGDFSTNQEYFPFINSLTE
                     NTVKGSLCMPVFGSMTSNSEFEFLTGDSVAMLPSNSIAYQFNVKPDAWTMVSTVKDQG
                     YRTVAMHPYPGENWNRSDKFLTKKEVC"
BASE COUNT         1001 a          701 c          856 g         1005 t
ORIGIN      
        1 agctgatcaa gcgtgaggtt tccgatggcg tgatcgctcc cggatatgaa ccggaagcac
       61 tggagatctt aaaatccaag aagaatggca actacaatgt gatccagatc gatcctgact
      121 atgtaccgga agaattagag cgtaagcagg tatttggtgt tgtatttgag cagggccata
      181 ataatttaaa gatcgacaaa gaaatgctta aaaatattgt tacagaaaat aaggatctga
      241 ccgaagaagc agctatggat ctggtaattt ccctgatcac tttaaaatac acccagtcca
      301 actccgtatg ctttgtaaag ggtggacagg ctattggtat cggtgcagga cagcagtcca
      361 gagtacattg taccagactg gcaggcagca aggcagataa ctggtattta cgtcagaacc
      421 caaaggtatt aaacctgcca tttattgacg gcatccgcag agcagaccgt gacaatgcca
      481 ttgacttata cattggtgaa gattatatgg atgttctggc tgacggaaga tgggagaaga
      541 tctttaagga aaaaccggaa gtatttacaa gagaagaaaa gagagcatgg ttagacggtc
      601 ttacagacgt agctttaggt tctgatgcct tcttcccatt tggagataac attgacagag
      661 cacataaaag cggtgtaaaa tatgtggcac agccaggcgg ctctgtaaga gatgatcagg
      721 ttattgatac ctgcaaccag tacggcatgg taatggcatt taccggtatc cgtctgttcc
      781 atcactagaa actgctttga tcaggatgat cagaaactga ccacaggcag aaatacccct
      841 ggctgagatc aagggggacg aatacttatg atgaaagatc ttaccagaga gcatccggat
      901 aaaattttat ggcgctttct gctgcctatg ttagtcagcg taatgtttca gcagatctat
      961 aatattgcgg acagctgtat tgccggccgc tttgcaggag aggacgccct ggctgcagtc
     1021 ggggcgtctt atcccattac agttatattt atggcgtttg ccgtagggac caacctggga
     1081 gcctctgttg tggtttccag gctttttggc gctaaggata tggcaaggat gaaatccgcg
     1141 gtaagtacgg cttttgttgc ctgcgctttt atgagctttt tcctcacact catcggctat
     1201 ttcttctgcg gcagtatgat gagggctgtc cacacaccgg aaaatatctt tgcagacgga
     1261 cagttatact taaagattta tgtttacggc atcacatttt tacttctgta taatgtatgt
     1321 accggtattt tcacagcatt gggagactca aaaacacccc tgtatttcct gatcggttca
     1381 tctgtgggaa atatcatcct ggattattat tttgtagcct gtgtaggact gggggtagca
     1441 ggtgttgcct gggctacttt tattgcccag ggtatttctg ctattttagc acttacattt
     1501 ctggcaatgc gcctgaaaaa gataaactgt gagggaactt ttcagtggct ggaccttgta
     1561 ctgttagccc agatcgctgc cattgccatt ccaagcatta tgcagcaaag cgttttatcc
     1621 attggaaata tgtttgtcca ggagatcgta aaccgctatg gttccgcggt gatcgcaggt
     1681 tattccggtg ctatcaagtt aaatactttt gctatcaatt catttatggc tcttggaagc
     1741 tgtctttcca gctatacggc ccagaacctg ggagcaggaa agaaagaacg gctgcctttg
     1801 ggatttcgta ccggtatccg tttatcagta attgcttccc ttccttttat agtcctttat
     1861 ttctttgcca gcagacagat gatggggctg tttttaaatg actccagtat cgatgccatc
     1921 aatgcaggac agaaattttt acatattgta agtcctatgt attttatgat atctattaag
     1981 ttaatgacag acggcattat ccgcggggca ggggccatgc attattttgt aatggctaca
     2041 gtaccggatc tgatcctgcg tatcctggta gcattgcttt tgacacagta ttttggaagt
     2101 acaggcatct ggatggcatg gccttttgga tggatcgcag caacggtcct gacgatcatt
     2161 ttctacagac gcattatcac aggcaagtat gtgatacgcc tgtagcagct ttgccatata
     2221 gtacccggtt gtaacaggat tggacagatc cgttcagatt gattcttgaa agtggttaca
     2281 aactgtgtta aaatttttaa tagtatgcaa aaaagtatgc gagaaatgat atcccagata
     2341 agtggcgata caggaagaga aggatagacg ccggtggcag aagagacaag aaattcagag
     2401 aatacaactg cagagagtac aaccacaaaa gaagaaaccg taaaaaaaga aaatatcatc
     2461 tggaaactat gtaaatatat gataaaaggc acaggctatc tgtgcctttt tcttgttccc
     2521 tgtttatctt attatctatt tgagtatgta acgggaaatc tggaaaatat ttccattttt
     2581 atggcggctt tgaatatatg ctgggcttat gtattgtacc ttgtacttac cggtattaca
     2641 ggaaccacaa ggatatctgt gccggtcgtg tccattgccc tgctgatcct ttcttttgcg
     2701 gaaacctttg tagtagattt tcgtgaccgg ccgatcatga tctgggatgt gctggcagtt
     2761 cgcactgcca tgactgtgtc gggaaattat gaatttgatg ttacaaagca gatgatacag
     2821 gcagccaaag ctgtgatcgg agccaatatg atcctctggt tcttcccggt ccacgtaaaa
     2881 ggcctcaaaa aacggctggt ctttggcggc acctgtatag gaacagcagc agcttttgta
     2941 tttggcttct ttcacagtgt agttcctgcc catcagatgg ggatcaatat gtgggcggtc
     3001 aacgacacct atgactcctg cggttatatt ttatctacgg ctatgtccct tcagtatgtg
     3061 gtgaaaaaac ctcctgtaga atacagccat ggaaaactgg aagccattta taaagaatta
     3121 acggagaaag aagagcagga aaaggaagca aaggccgaag gcacacagaa aacgggagcc
     3181 ggggaagaga cggtgcagcc tgtaaatctg atctgtatta tgaatgaaag cctttctgac
     3241 ctgcgggtgg ttggagattt ttccacaaac caggaatatt ttccttttat caacagcctg
     3301 acagaaaata cagtaaaggg cagtctttgt atgcctgtgt ttggttctat gaccagcaat
     3361 tctgaattcg agtttttaac gggggattcc gttgccatgc ttccgtctaa ctccattgcc
     3421 taccagttta atgtaaaacc agatgcatgg actatggtaa gtacagtgaa ggatcaggga
     3481 taccgtacag tagccatgca tccatatccg ggagaaaact ggaacaggtc agacaaattt
     3541 ttaacaaaaa aagaagtctg caa
//
LOCUS       DBMDUA010000248         3554 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9272_length_3554_cov_2.003475, whole genome shotgun sequence.
ACCESSION   DBMDUA010000248 DBMDUA010000000
VERSION     DBMDUA010000248.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9272_length_3554_cov_2.003475"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(62..787)
                     /gene="cobK"
                     /locus_tag="ACZPCL_12790"
     CDS             complement(62..787)
                     /gene="cobK"
                     /locus_tag="ACZPCL_12790"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR00715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HIR1163380.1"
                     /translation="MAKKHVILFAGTTEGRILANWLDEQSGVTGIVCVATEYGAELLE
                     EEMSLKHLTVHCGRLDEGAMEAFLKKESPSLVIDATHPYAQVVTANIRKACEKYPDIC
                     LLRCLRKESEDEVKDSKGDKNGIIHVKNTAEAVRYLSEKEGNIFLTTGSKELVLWQEL
                     PGHLERIFARVLPVEASVHICRELGYSGRHIIAMQGLFSAEMNYIQLKEFQCSYMVTK
                     DGGDTGGFKEKMQAAKKAGATHP"
     gene            complement(789..2330)
                     /gene="cobM"
                     /locus_tag="ACZPCL_12795"
     CDS             complement(789..2330)
                     /gene="cobM"
                     /locus_tag="ACZPCL_12795"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HIR1163381.1"
                     /translation="MVHIVGAGPGAEDLITVRGLRLLKQADIVIYAGSLVNPGLLKET
                     KQGAVIYDSAKMTLEQVMEVIEQAWKEQKEVVRLHTGDPCLYGAIREQMDAMDKLGIT
                     YDICPGVSSFCGTAAALQMEYTLPGISQSVIITRMAGRTPVPERESIRQFASHQATMV
                     IFLSTGMLKELSKELMAGGYSPDTPAAIVYKATWPEEKTVRTTVAELAEAAEREHITK
                     TALIVVGNTVAQNGYDRSKLYDPGFTTEFRMAESSHSGKIVSAVPEIAASGKLYVVGM
                     GPGSLDGMTKEAFKAIGDCQVIAGYTVYADLVKPYFPDKEYLTTPMTKEEARCRMAFE
                     CCMEGKDTAMICSGDSGVYGMAGLILELSAQYPGVEIKMIPGVTAACAGAAGLGAPLT
                     HDFAVVSLSDRLTPIEMIWERIEKAAQADFVICLYNPRSKGRPDYLKQACERIMKYRS
                     PETVCGVASCIGRDGEEMKVISLKELADYPADMFTTVFIGKTSTVKIGKWMVTPRGYL
                     QKKEK"
     gene            complement(2368..3552)
                     /gene="cbiD"
                     /locus_tag="ACZPCL_12800"
     CDS             complement(2368..3552)
                     /gene="cbiD"
                     /locus_tag="ACZPCL_12800"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HIR1163382.1"
                     /translation="MYVYKNQKKLRWGYTTGTCGTAASMAAALMLLGQKRVGQVKVST
                     PKGLDLDLEIEEIRFTKEQAVCAVRKDSGDDPDVTNGIAIYSRVSFRDDKPETEGYIW
                     ENEVIRLHLTAGEGVGIVTKPGLACEVGKPAINPVPREMIFTHVERACRQHGFQGSLH
                     IEIFIPQGIEISKKTFNPRMGIKGGISLLGTSGMVEPMSEKALTDTIQLELHQKHVAG
                     LKSMILTPGNYGESFLRDELNVNLDYAVKCSNFIGDSLDMAVQENIEEVLLVGHGGKL
                     IKLAAGVMNTHSSVADGRMETLAAWGGACGAGPELIREILESVTVDQGLKLLETVPGL
                     REKVMEQVVRRAWEHLTLRAGESMKVECILFTNERGILGMSPGAMDMLKRILAQDMTK
                     LD"
BASE COUNT          789 a          996 c          686 g         1083 t
ORIGIN      
        1 ctggttttgg cgtgacagtt cttcggtctg ttcttccagt agttcttttt gcctctctag
       61 ttcatgggtg cgttgctccc gctttttttg cagcctgcat cttttcttta aagccgcctg
      121 tatctccccc gtcctttgtt accatataag aacattgaaa ttccttcaat tgtatataat
      181 tcatttccgc tgaaaacagt ccctgcatgg caatgatatg tcttccgctg tagcccagtt
      241 ccctgcagat atgaacagaa gcttccacag gaaggactct tgcaaaaatc cgttctaaat
      301 gtcccggaag ttcctgccac aaaaccagtt ccttgcttcc tgtagtgagg aagatatttc
      361 cttctttttc agaaagatac cggactgcct ctgctgtatt tttcacatgg ataatgccat
      421 ttttatcgcc tttgctatct tttacctcat cttcactttc ttttcgcagg cagcgcagaa
      481 ggcagatatc cggatatttt tcacaggctt tccggatatt ggcagtcacc acctgggcat
      541 agggatgggt ggcatcgatc actagagacg ggctctcttt tttcaaaaaa gcttccatcg
      601 caccctcgtc caggcgaccg cagtgtaccg ttaaatgctt taaagacatt tcttcttcca
      661 gaagctctgc accgtattcc gttgccacgc agacgattcc ggtaacacct gactgttcgt
      721 ccagccagtt agccagtatc cgcccttctg ttgttcccgc aaagaggatc acatgctttt
      781 ttgccatgtt atttctcctt cttttgcaga tagcctcttg gtgtcaccat ccatttgccg
      841 atctttacag tactggtttt tccaatgaat actgtcgtaa acatatccgc cggataatca
      901 gccagttctt ttaaagatat taccttcatc tcttccccat ctcttccaat acaggaagct
      961 acaccgcaga ctgtttccgg tgaacggtat ttcatgatcc gctcacaggc ctgctttaaa
     1021 tagtccggtc tgcctttgct gcggggatta tataagcaga taacaaaatc tgcctgggct
     1081 gccttttcta tgcgctccca gatcatttct atgggtgtta aacggtcact taagctgacc
     1141 actgcaaaat catgagtcaa cggtgctcca aggcctgcag caccggcaca ggcagctgta
     1201 accccgggta tcatcttgat ctctactccc ggatactgtg ctgacagctc caggatcaga
     1261 cctgccatac cgtaaacacc gctgtcccca ctgcagatca tggctgtatc tttgccttcc
     1321 atacagcatt caaaggccat acggcaccgt gcctcctctt ttgtcatagg cgtggtaaga
     1381 tattctttgt ccgggaaata gggctttaca aggtccgcat ataccgtata tccagcgatc
     1441 acctggcagt ctccaatggc tttaaacgct tcttttgtca ttccatctaa ggaacccggt
     1501 cccataccta ctacgtagag ttttcctgat gctgctattt ccggcacagc cgaaactatt
     1561 ttcccgctgt gggagctttc tgccatacgg aattctgttg taaatcccgg atcatacagt
     1621 tttgaccggt catagccatt ttgtgccact gtatttccaa ctacgatcaa ggctgttttt
     1681 gtaatatgtt cacgctccgc tgcctctgcc agctctgcca cagtggtacg cacggttttc
     1741 tcttccggcc aggttgcctt gtatacaata gcagctggtg tatccgggct gtacccccct
     1801 gccattaatt cctttgacag ttcttttaac attcccgtac ttaaaaagat caccatggtt
     1861 gcctgatgag aagcaaactg gcggatgctc tctctctccg gcaccggtgt cctgcctgcc
     1921 attctggtaa taattacgct ttgggatatt cccggaagag tatactccat ctgtaaagcg
     1981 gctgctgtgc cgcagaaaga actgactccc ggacagatat cataagtgat ccccagcttg
     2041 tccattgcat ccatctgctc tctgatggca ccgtagagac agggatctcc tgtatgcagg
     2101 cggactacct ctttttgctc tttccatgcc tgttctataa cttccataac ctgttccagg
     2161 gtcatttttg cgctgtcgta gatcacagcc ccctgttttg tctcttttaa taaacctgga
     2221 ttgaccaggg aacctgcata aataacgata tctgcctgtt ttaaaagtct cagtcctctt
     2281 actgtgatca ggtcctctgc tcccggacca gctcctacaa tgtgtaccat atttctcttc
     2341 ctttccggaa tttattttcg tttattttca gtccagtttc gtcatatcct gggccagtat
     2401 cctttttaac atgtccatgg ctccgggaga cattcccagt atccctcttt cattggtaaa
     2461 caggatacat tctaccttca tggactctcc tgcccgcaat gtcagatgct cccaggcccg
     2521 tcttaccacc tgttccataa ctttttcacg aagccccggc actgtctcta aaagttttaa
     2581 tccctgatct acagtaacgg actctaaaat ctcccggatc agctccggtc ctgcaccgca
     2641 ggcaccaccc caggctgcca gagtttccat tcttccatct gctacagagg aatgggtgtt
     2701 catcacacca gctgccagct tgatcagctt tcctccatgt ccgactaaca gtacctcctc
     2761 aatattttct tgcactgcca tgtccagaga gtcaccaata aaattactgc actttacagc
     2821 gtaatccaga tttacattca actcatcccg taaaaagctt tccccataat ttcccggtgt
     2881 cagaatcatg ctttttagcc cggccacatg tttctgatgc aattctaact gtatggtatc
     2941 ggtaagtgct ttctcactca taggctctac catgccactg gtccccaaaa gggagatacc
     3001 gcctttgata cccatacggg gattaaaggt ctttttgctg atttctattc cctgaggaat
     3061 aaatatttca atatgcagac tgccctgaaa tccatgttgt ctgcaggctc tttccacatg
     3121 ggtaaagatc atttcccttg gtaccggatt aatagcaggt tttcctactt cacaggccag
     3181 tcccggcttt gtaacgatac ccacgccctc tcctgctgta agatgaagcc ggataacctc
     3241 attttcccag atatagcctt ctgtctccgg tttatcatcc ctaaatgata ctctggaata
     3301 gatagcgatc ccattggtca catccggatc atctccggag tcctttcgca cagcgcagac
     3361 tgcctgctcc tttgtaaaac ggatctcctc gatctctaga tccaggtcta gccccttagg
     3421 tgtagagact ttcacctgac ccacccgttt ctgtccaagg agcataagag ccgctgccat
     3481 agaagccgcc gtcccgcagg taccggtggt atagccccat cttaactttt tctgattttt
     3541 atatacatac attt
//
LOCUS       DBMDUA010000249         3552 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9279_length_3552_cov_1.787598, whole genome shotgun sequence.
ACCESSION   DBMDUA010000249 DBMDUA010000000
VERSION     DBMDUA010000249.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9279_length_3552_cov_1.787598"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(329..1510)
                     /locus_tag="ACZPCL_12805"
     CDS             complement(329..1510)
                     /locus_tag="ACZPCL_12805"
                     /inference="COORDINATES: protein motif:HMM:NF040203.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR1163383.1"
                     /translation="MKLNKKTFMIAGAAAMLTIGAAIPSFAATSGWATEGDSWCYLDA
                     QGNKVTNVWKKSGNDWYYLDSDGLMATDTWVDDTSYVDIYGVRVTNRWIYTEAGTDNA
                     PNSEGGWYYLDAAGKAVSDGWKTINNKKYYFDSDGTMQYGWYTDGSDTYYLGDENNGA
                     AATGWLCLDFDKDNVPSDGDVSEQETIGSDTAKWFYFLSNGKAVKADNDTYTSKTIGG
                     KKYYFDSNGVMLTGWVSMANEGDSSDVDPTGISSFKYFGDDNDGQMSKGWKYLSDYPE
                     DSDDSSNIVEATASNAQRYGSSDGNGSWYYFDSNGKPKYLKADATSMSKATVRINGQS
                     YFFDEYGKMQYGLIGIDFGDGTEHSAYFGNSESDGKMRTDKQTSVTEDSGDRTTFFFK
                     K"
     gene            complement(1849..2748)
                     /locus_tag="ACZPCL_12810"
     CDS             complement(1849..2748)
                     /locus_tag="ACZPCL_12810"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR1163384.1"
                     /translation="MNTDFMKGVIVPILTPIDENEWIDEIKMREQVDFVINGGVSGIL
                     AFGSNGEFYVIEEDEMERGLKIIVDQAAGRVPVYFGIGAISTKKCVRLAKMAVACGAA
                     GVSVLQPMFLKPTETELFCHFKTIAEAIPDTPMLLYNNPGRVGYTMSGNLVERLAHEV
                     KNIVGMKDTSGDITQTSEFIRRTRDVDFKVFGGKDTLLYASLCHGAVGGVCTAANFMP
                     ELITDVYNKYIAGDLKGSLEAQFKLNPVRLSMDGASFPVAAKDMANLRGREIGDPYKP
                     NLPTPEGPVLDKIRKEMKTAGLI"
     gene            complement(2764..>3552)
                     /gene="garR"
                     /locus_tag="ACZPCL_12815"
     CDS             complement(2764..>3552)
                     /gene="garR"
                     /locus_tag="ACZPCL_12815"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HIR1163385.1"
                     /translation="VAELVACGATSAKSSKEVAAQVEVVITMVPNSPHVRAAVLGKDG
                     VAEGAKPGLVLIDMSSIDPTESKKIGEECMKYGIEMLDAPVSGGEPKAIDGTLSVMVG
                     GKKDLFDKYYDMLMVMAGSVVYVGDLGSGNVAKLANQMIVAINIAAVSEALIFAKKAG
                     TDPELVYQAIRGGLAGSTVMDAKAPMMLAGNYKPGFRIELHIKDLNNALNAAHAISSP
                     APLTAQLMEVMQFLRSEGCDKEDHSSIIKYYEKIAGASVVKDKE"
BASE COUNT          927 a          876 c          624 g         1125 t
ORIGIN      
        1 acctcctgat ttccgctcac aaaggggttt tgtcgttcat gttaaaatgt acttacctta
       61 cttcgcggct ttgttcgtcg ttgcttcata ctataaacca attattttca tttgtccagt
      121 ctatatattg taattgctac agctttttag ttgtggttca gaatgtaaga aggggatttt
      181 tgctgttaga tggtatcgct ttaacgtgtg atgaattagt ctcggctaat atacgggtcg
      241 tatattgaca gcaaaaaacc tgctgcaacc atcttaagaa ggttatgcag caggcctatt
      301 ttctgcctga aacaggagtg tctgtctttt attttttaaa gaagaaggta gtacggtcac
      361 cactgtcctc tgttacactg gtctgcttgt ctgtacgcat ctttccgtca gactcactgt
      421 ttccaaagta agcgctgtgc tctgttccat cgccaaagtc aataccgatc agaccatact
      481 gcatttttcc atattcatca aagaaataac tctgtccgtt gatccttacc gtagcctttg
      541 acatggacgt tgcatctgcc tttaagtatt ttggctttcc gttggagtca aagtaatacc
      601 agctgccgtt tccgtcactg ctgccataac gctgggcatt gcttgcagtc gcttctacaa
      661 tattagaaga atcatcggag tcttccggat aatcggaaag gtacttccag cccttgctca
      721 tctgaccgtc attatcatca ccaaaatatt taaaagaaga aataccggtc ggatccacat
      781 cggaactgtc accttcattt gccatagata cccatcctgt aagcatcacg ccgttggagt
      841 caaaataata tttctttccg ccgatggtct tactggtgta agtatcatta tccgccttta
      901 ctgccttgcc atttgacagg aagtaaaacc atttcgctgt atcgctgcct atggtctcct
      961 gctccgatac atctccgtca cttggcacat tgtccttgtc aaaatcaagg cagagccagc
     1021 cggtagctgc cgcaccatta ttttcatctc ccagataata agtatcgctt ccgtctgtgt
     1081 accagccata ttgcatggtg ccatcagagt cgaaataata ttttttattg ttaatggtct
     1141 tccagccatc gcttactgcc ttaccggctg catccaggta ataccagcct ccttcagaat
     1201 ttggggcgtt atcggttcct gcttctgtgt agatccagcg gtttgtgact ctcacaccgt
     1261 aaatatctac ataggaagta tcatctaccc aggtgtccgt tgccatcaga ccatctgagt
     1321 ccagataata ccagtcattt cctgattttt tccacacatt ggtcaccttg tttccctgag
     1381 catccaagta acaccagctg tccccttccg ttgcccagcc gctggttgct gcaaaagaag
     1441 gaatggctgc tcctatggta agcatggctg ctgctcctgc gatcataaat gtttttttat
     1501 tcagtttcat attgaaagct cctttctttc cctttcttag agggctatcc ttaagacacc
     1561 ctaataataa aaaaaagaat aaaagatttt tatgaaaaga aataaacaaa tcttaaactc
     1621 tgatcatccg gagttttcaa cgcttccttc gtattttttc atacaacaca aaaagactgc
     1681 tgccagacag atccaaagat gggcttcttt gtatctgttt cgcaacagtc tttttattga
     1741 aggtgagagt acatttcatt ttgttccatg caataaggat ttctttcgga acactttgta
     1801 tactactctc tggttttaac gtttttaggg ggataatata aacaaagttt aaattaatcc
     1861 cgcagtcttc atttctttcc tgatcttatc caggaccgga ccttcaggcg ttggaaggtt
     1921 tggtttatac ggatcaccga tctctcttcc ccgtaaattt gccatatctt ttgcagcaac
     1981 tgggaaactt gcgccatcca ttgacagacg tactggattc agcttaaact gtgcctctaa
     2041 agatcctttc aggtccccgg caatatattt attgtataca tctgtgatca actccggcat
     2101 aaaattagct gctgtacata cgccacctac tgctccatga cataaagacg catacaataa
     2161 tgtatcttta ccaccgaata ccttaaaatc cacatctctt gtacggcgga taaactcaga
     2221 agtctgggta atatcaccac ttgtatcttt cattcccaca atatttttaa cttcatgagc
     2281 cagacgttct accagattac cagacatggt atatcccaca cgtcccggat tattgtataa
     2341 aagcattggc gtatcaggga ttgcctctgc aattgtttta aagtggcaga acaattctgt
     2401 ttctgtcggt tttaagaaca ttggctgtag aacagaaaca cctgctgccc cgcatgcaac
     2461 tgccatttta gccagtcgca cacatttttt tgtactgatg gctccgattc cgaaatatac
     2521 aggaacacgg ccagctgcct gatctacaat aatcttcagt ccacgttcca tttcatcctc
     2581 ttcgatcaca taaaattcgc cgttgcttcc aaaggccagt attccactga caccgccatt
     2641 gatgacaaaa tccacctgct cacgcatttt gatttcatca atccattcat tttcatcaat
     2701 aggtgttaaa atagggacga ttacaccttt cataaaatct gtattcatca tcattctccc
     2761 agattattct ttgtctttta caacacttgc accagcaatc ttttcataat atttgataat
     2821 gctggaatga tcttccttgt cacagccctc tgatcttaag aactgcatta cttccatcag
     2881 ctgggctgtt aaaggagccg gtgaactgat agcatgagct gcatttaatg cattatttaa
     2941 gtcctttata tgcagttcga tacgaaatcc tggtttatag tttcctgcta acatcatagg
     3001 agcctttgca tccataactg tagaaccggc taatccaccg cggatcgcct gatataccag
     3061 ctctggatct gtacctgcct tctttgcaaa gatcagtgcc tctgatactg ctgcaatatt
     3121 aatagcaacg atcatctgat ttgccagttt agccacattt cctgaaccca gatctcccac
     3181 atatacaaca gagcctgcca taaccattaa catatcatag tatttgtcaa aaagatcttt
     3241 tttgccgccc accataacag ataatgtgcc gtcaatggct tttggttcgc cgcctgatac
     3301 aggtgcatct aacatctcga ttccatattt catgcactct tcaccgatct ttttgctttc
     3361 agtcggatca atagaactca tatcaatcag aaccagacct ggtttcgcac cttccgcaac
     3421 accatctttt cctaaaacgg cagctcttac atgaggagaa ttaggaacca tagtaatgac
     3481 tacttcaacc tgtgctgcta cttccttact gctcttagct gaagttgccc cacatgctac
     3541 cagttctgcc ac
//
LOCUS       DBMDUA010000250         3544 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9307_length_3544_cov_3.223352, whole genome shotgun sequence.
ACCESSION   DBMDUA010000250 DBMDUA010000000
VERSION     DBMDUA010000250.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9307_length_3544_cov_3.223352"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="ACZPCL_12820"
     CDS             <1..765
                     /locus_tag="ACZPCL_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIR1163386.1"
                     /translation="LDIEKGSFVAVLGHNGSGKSTFAKHINGLLLPTEGTVWVGEMDT
                     KDEEHIWDVRKTAGMVFQNPDNQIIGNIVEEDVGFGPENIGVETKEIWKRVDESLKAV
                     GMTAYRKQSPNKLSGGQKQRVAIAGVMAMRPQCIILDEPTAMLDPNGRKDVIRTVHEL
                     NKKEGITVLLITHYMEEAIDADRVIVMDDGKVIMDGIPKEIFSRVKELKEHGLDVPQA
                     TELAFELKEAGMPLTEGILSRQELVEQLTALLSDEK"
     gene            775..1641
                     /locus_tag="ACZPCL_12825"
     CDS             775..1641
                     /locus_tag="ACZPCL_12825"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIR1163387.1"
                     /translation="MSIKAVDLNYVYGNGTAFEQHALFDVNFEIQDGEFVGIIGHTGS
                     GKSTLIQHLNGLIKASSGALYYNGENIYEDKYDMKKLRSKVGLVFQYPEHQLFEIDVL
                     TDVCFGPKNQGFSEEEAKEKAKKALSLVGLGESYYSQSPFELSGGQKRRVAIAGVLAM
                     EPEVLILDEPTAGLDPRGRDEILDQISKLHTERKMTIILVSHSMEDVARYAGRLIVMN
                     HGQKVFDGTPREVFRHYKELEAIGLAAPQVTYLVHDLKEKGLDIDSDITTVAEAKEAI
                     LALWDKKRKRQV"
     gene            1644..2444
                     /locus_tag="ACZPCL_12830"
     CDS             1644..2444
                     /locus_tag="ACZPCL_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR1163388.1"
                     /translation="MLRDITLGQYYPVDSLIHRLDPRTKLFGTMVFILSLFVANNIWG
                     YLLGTIALAAVIVLTKVPVRFILKGLKAIVILLLISVSFNLFLTPGRELVRFWIFKIT
                     WEGIRLAGFMAVRLIYLVVGSSLMTLTTTPNQLTDGLEKGLGFLKRFHVPVHEISMMM
                     SIALRFIPILVEETDKIMKAQMARGADFESGNLVQKAKAMIPLLVPLFISAFRRATDL
                     AMAMEARCYRGGEGRTKMKPLQYSQKDHEAYMVFALYFVLIIASRVYL"
     gene            2530..3276
                     /gene="truA"
                     /locus_tag="ACZPCL_12835"
     CDS             2530..3276
                     /gene="truA"
                     /locus_tag="ACZPCL_12835"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR1163389.1"
                     /translation="MKKRVLLRVAYDGTNYHGWQVQPGAVTIEQVLNEKLTELLGEPI
                     EVIGASRTDSGVHAMGNVAVFDTEHRMPADKICFALNQRLPEDIRIQSSMEVEPDWHP
                     RKHHCTKTYEYKILNRKMEVPSLRLYTHFCHFDLDLDQMRQAAKLLTGEHDYKSFCNV
                     RTQVLDTVRTVYSIDIEKDSNDVITIRVRGNGFLYNMVRILAGTLMAVGMGQRKPEEM
                     PDILAACDRAAAGPTAPAKGLMLVGMEYQD"
BASE COUNT         1116 a          656 c          900 g          872 t
ORIGIN      
        1 ctggatatag aaaagggaag ttttgtggca gttttaggac ataatggttc cggaaaatct
       61 acttttgcaa agcacatcaa tggtcttctc cttccaacag aaggtactgt atgggtgggt
      121 gagatggata ccaaagatga agaacacatc tgggatgtgc gcaaaacagc aggaatggtg
      181 ttccagaacc cggataacca gatcattgga aatattgtag aagaagatgt aggtttcgga
      241 ccggaaaata ttggtgtgga aacaaaagag atctggaagc gggtagatga aagcctgaaa
      301 gcagtaggga tgacggcata cagaaaacag tctcccaaca agctgtccgg cggtcagaaa
      361 cagagagttg ccattgccgg tgttatggcc atgcgtcctc agtgtattat tttagatgaa
      421 cctactgcca tgttggaccc aaatggacga aaagatgtaa tacgcacggt tcatgaactg
      481 aataaaaaag aaggaattac agtccttctt attactcatt atatggaaga agccattgat
      541 gcagaccgtg tgatcgttat ggacgacgga aaagtgatca tggatggaat accaaaagaa
      601 atcttttccc gtgtgaagga attaaaggaa catggactgg atgtacctca ggcaacagaa
      661 ctggcatttg agttaaaaga agcaggaatg cctttgacag aggggatctt aagcagacag
      721 gagctggtag aacagcttac agcgttgctt tcagatgaaa aataggagaa taacatgtcg
      781 ataaaagcag ttgatctgaa ttatgtatac ggaaatggta cggcctttga gcagcatgcc
      841 ctttttgatg tgaatttcga gatccaggac ggggagtttg taggcattat cggacatacc
      901 ggttccggaa aatccacatt gatccagcat ttaaacggac tgatcaaggc cagctccggc
      961 gctttgtatt ataatggaga aaatatttac gaagataaat atgatatgaa aaagcttcgt
     1021 tccaaggtag gactggtatt tcagtacccg gaacaccagc tttttgagat cgatgtactg
     1081 acagatgtat gtttcggacc gaaaaaccag ggattttctg aagaagaagc aaaggaaaag
     1141 gcgaaaaaag ctttaagtct ggtaggcctg ggtgagtctt attacagcca gtcaccattt
     1201 gaactgtccg gtggtcagaa gagaagagtt gccatagcag gtgtgcttgc aatggagccg
     1261 gaagtcctga tcttagatga accaacagca ggtcttgacc cgcgaggaag agatgagatc
     1321 ttagaccaga tctcaaagct gcatacagag cggaagatga ctattatcct ggtatcccac
     1381 agtatggaag atgttgccag atatgcaggc agactgatcg ttatgaacca tggacagaaa
     1441 gtgtttgacg gaactccaag agaagtattc cgccattata aagaactgga agccattggt
     1501 ctggcagcgc ctcaggtaac ttatctggtc catgatctga aagaaaaagg actggatatt
     1561 gacagtgata ttactacagt ggcagaagca aaagaggcga tcctggcatt gtgggataaa
     1621 aagagaaagc ggcaggtata aaaatgctga gagatataac attaggacaa tattatccgg
     1681 tggattccct gatccatcgt ctggatccaa gaaccaaact ttttgggaca atggtgttta
     1741 tcctgtccct ttttgtggca aataatatat ggggatattt attagggacc atagcactgg
     1801 ctgctgtgat cgtactgaca aaagttccgg tgcgttttat cttaaaggga ttaaaagcca
     1861 ttgtgatctt attactgatc agtgtcagtt ttaatctatt ccttacaccg ggacgcgaac
     1921 tggtacgttt ctggatcttt aagatcacct gggaaggaat ccgccttgca ggttttatgg
     1981 cagtgcgcct gatctacctg gtagtaggtt cttcccttat gacattgact accactccga
     2041 accagctgac agatggtctg gaaaagggac tgggattttt aaagcggttc catgtaccgg
     2101 tacatgagat ctctatgatg atgtctattg cactgcgttt tattcctatt ttagtagagg
     2161 aaacagacaa gatcatgaag gcccagatgg caaggggcgc tgattttgag tctggaaatc
     2221 tggtacagaa agcaaaagcc atgatcccgc tgttagtacc gttatttatc tctgctttcc
     2281 gcagagcaac agacctggca atggcaatgg aggcaagatg ttaccgcggc ggcgaaggaa
     2341 ggaccaagat gaaaccgttg cagtacagcc agaaagacca tgaggcatat atggtctttg
     2401 cactgtattt tgtgctgatc attgcttcca gggtttatct gtaaatgtat cctgtataca
     2461 ataaaagata agaaacagta taagcagtaa aatatatggg acgcaggaaa gtataaggag
     2521 aaaagaaaca tgaaaaaaag ggtccttctg agagtggcct atgacgggac caactatcac
     2581 ggctggcagg tacagccggg agcagtgacc attgaacagg tgttaaatga aaaactgaca
     2641 gagcttttag gggaacctat tgaagtgatc ggagccagcc gcacagactc cggcgttcat
     2701 gcgatgggaa atgtggcagt atttgacaca gaacatcgta tgcctgcgga caagatctgt
     2761 ttcgctttaa accagcggct cccggaagac atccgcattc agtcatctat ggaagtggag
     2821 ccggactggc atccgagaaa acatcactgc accaaaacgt atgaatataa aatattaaac
     2881 cgcaagatgg aagtaccgtc cctgcgtctg tacacccatt tctgccattt tgacttagac
     2941 ctggatcaga tgcgccaggc tgccaaactg ctgacaggag aacatgatta taaaagtttc
     3001 tgcaatgttc gtacccaggt attagatact gtgcgtactg tatacagtat tgatatagaa
     3061 aaagacagta atgatgtgat caccatacgg gtacggggaa acggcttcct ttataatatg
     3121 gtccgcatcc ttgcaggaac ccttatggca gtgggaatgg gccagagaaa accggaggaa
     3181 atgccggata tccttgcagc ctgtgaccgg gcggcggcag gtccaacagc gcctgcaaaa
     3241 ggtctgatgt tagtgggaat ggaatatcag gattaaaaat ggaaaaatcc ttgaaaatat
     3301 aaggatttag ccaagaaaat ggcttgacat ggcaaaacag atattatata ataagaaact
     3361 gtgacgttga gagtaaatgc tcatccaaag ccccggagcg gagcatttca ttgataaata
     3421 cttcccaata tttatcacat atagcgatca tctgaaaaga tgtaatttag gttgaaatta
     3481 acaggaggtt aaccaatgaa gagttttatg gctagtccag cgacaattga gagaaaatgg
     3541 tacg
//
LOCUS       DBMDUA010000251         3510 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9398_length_3510_cov_3.604869, whole genome shotgun sequence.
ACCESSION   DBMDUA010000251 DBMDUA010000000
VERSION     DBMDUA010000251.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9398_length_3510_cov_3.604869"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(334..1242)
                     /locus_tag="ACZPCL_12840"
     CDS             complement(334..1242)
                     /locus_tag="ACZPCL_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR1163390.1"
                     /translation="MEAILMRAGSFMAVIILGYVLKKVGFFKEEDFYVMSKIMVKITL
                     PASIIVNFSQTKMEPSMFLLCLLGFGGGILYMALGWIMGGKDPDEKGFQILNLSGYSI
                     GSFTMPFTSSFFGPAGVVVTSLFDTGNAVIALGGSYSIGAMVKNREKKFSFIPLCKTL
                     LCSVPFDAYLFMYILYWLHLSLPAPVLNIAQLVANANAFLAMLMLGVGFKLSGDRTQM
                     SKIVKMLGVRYGVAVLTAAAFYFLLPFSLEYRKALAVLVFSPIAAAAPAFTADLKGDI
                     NLASAVNSLSIIISMFCITCVLIAVL"
     gene            complement(1706..3403)
                     /locus_tag="ACZPCL_12845"
     CDS             complement(1706..3403)
                     /locus_tag="ACZPCL_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1163391.1"
                     /translation="MNKRILIIGGVAGGASVAARARRLDAGAEIIMFERGQHVSFSNC
                     ALPYYLSGVVGNSDALVMMSPEKFKKSYDIDARVNSEVMAINRDKKTIEIKNTLTGDI
                     HEEAYDVLVMAPGASPVKPRSIGGIDGKNVYTVRNVADIVHLKDGLEAAGAENTAVIG
                     GGFIGIEVAENLAKAGKKVTVVEAMSQILAPFDYDMVQMLHKELLDNGVSLIVDDGVK
                     TIEEKQITLASGKTVPADAVILAIGVAPETGLAKAAGLEIGETGGILVDHNYRTSDKN
                     IYAVGDAIEVFDRMTGKPSRLALAGPALRQARAAADAMYGIPNDNRGVIGSCALRVFG
                     LNAAATGLNERNARKAGIPCDSVYVIPGDKVGIMPGSAPMHFKLVFEVPTGRILGAQA
                     IGRGNVDKRVDVIAAMVSMNGTIRDLKNLELCYSPVFGTARDVVNQAALVAENILYGR
                     FRQVPVTQVRSLVEEQAFIVDVREKGEYEAGHLKGSVNIPLSELRERTDEIPKDRPVY
                     LHCRSSQRSYNALMALQGRGYENVVNISGSFLGICLYEYYNDVAMGREKIVTEYNFA"
BASE COUNT          977 a          911 c          696 g          926 t
ORIGIN      
        1 cccaagatat cctctgatga gtgagatcaa ggaaatgtat ttaagagctt actacggaaa
       61 ataatcattt atataaatcg aacccattgt gggtaaaaac ctttatcctt tgtgtttgga
      121 gcagaccgtc ggcctttcgg ggcaggcggt ctgttcgtat gtatgaggat caggtacggg
      181 ttattgtata tggtaggcac ttttggaacc gaaagtgccg tacgataacg tagtggtagc
      241 gggaatttgc cgtatttaaa tgcgaagcca cggcaaattc cgttgctggt catgcaacgc
      301 atgaacagta actacaacat atatctatca tttctataga actgcgatca gcacacaggt
      361 aatgcagaac atactgataa tgatggaaag agaatttacc gcgcttgcca ggttaatatc
      421 ccctttcagg tctgccgtaa atgcaggcgc tgctgcggca atagggctga acacaagcac
      481 tgccagagcc ttacggtatt ccagggaaaa aggaagcaga aagtaaaatg ctgctgctgt
      541 taaaacagcc actccatagc ggacacctaa cattttcacg atcttgctca tctgtgtccg
      601 gtctcctgac agcttaaatc caacacctaa cataagcatg gcgagaaaag catttgcatt
      661 tgccaccagc tgtgcaatgt ttaatacagg tgccggcagt gacagatgaa gccagtataa
      721 aatatacata aaaagataag catcaaaagg aacagaacag agaagtgttt tgcacagtgg
      781 gataaatgaa aatttctttt ccctgttttt aaccatagca ccaatactgt aagaaccgcc
      841 taaagcgatc acggcatttc ccgtatcaaa cagactggta acaactacac cggcaggtcc
      901 aaaaaagctg ctggtaaatg gcatggtaaa gcttccgata ctatagccgg aaagattcag
      961 gatctgaaat cccttttcat ctggatcttt tccacccatg atccatccaa gagccatata
     1021 taaaataccc cctccaaaac ctaacaggca tagtaaaaac atggatggct ccatttttgt
     1081 ctgtgagaaa ttgacaatga tggatgcagg aagtgtgatc ttcaccatga tctttgacat
     1141 aacataaaaa tcttcttcct taaaaaatcc tacttttttc agaacatacc ccaagatgat
     1201 cacagccata aaagaaccag cccgcatcag tattgcttcc ataagtttct tcccccaaaa
     1261 cccctgatgt tctctcggaa tctttctgtg cctgattttg tgagaagatt ttttgtcagt
     1321 tgtgcccaaa tttctgaggt gtacgcggtg cgtacattga tgaaattcgg gtgcgactgg
     1381 cggaaaatca gccacaaaag caggtgcagg aaagactccg agagaacatc ctgatatttt
     1441 ccaataactg gaacttaaca ctttctttat agtaacacat ctcatcgttc aatagcaact
     1501 gccccttaaa caaaaatacc gccccttttc aatagcctgt tcatgagaac tacaagctga
     1561 tctgccgatt cgcaggttct gtcgccggca gcttctttcg gatgcatgac aacagcgaaa
     1621 aacgcagaat gtgcgttttc cataaaaact tactccaaca cacattctgc gtcaatctaa
     1681 aagttgtata aaaatatcag aatatttatg cgaaattata ttccgtcacg attttttccc
     1741 gccccatggc cacatcattg tagtactcat acaggcagat tccaaggaat gatccggaaa
     1801 tattaaccac attctcatat ccccgtccct gaagcgccat cagcgcattg tagcttctct
     1861 ggctggaacg gcaatgcagg tataccggac ggtctttcgg aatttcatcc gttctctcgc
     1921 gaagctcact taaaggaatg ttcacagaac ccttcaaatg tccggcctca tactctcctt
     1981 tttcccgtac atccacaatg aacgcctgtt cctccacaag gcttcttacc tgtgtaaccg
     2041 gaacctggcg gaaacgtccg tacagaatat tttcagccac cagagccgcc tgatttacca
     2101 catctctggc tgtgccaaat actggtgaat aacacagctc caggttcttt aaatcccgga
     2161 tggtgccatt catggaaacc atggctgcaa tcacatccac ccgtttgtcc acatttcctc
     2221 ttccgatggc ctgagcaccc aggatccttc cggttgggac ctcaaacacc agtttaaagt
     2281 gcataggtgc acttcccggc ataattccca ccttgtcacc tggaatcaca taaacagaat
     2341 cgcagggaat tccggctttg cgggcattcc gctcattaag accggttgct gctgcattta
     2401 agccaaatac acgcagtgca caggaaccga tgacacctct gttgtcattg ggaatcccat
     2461 acatcgcatc cgccgccgcc cgcgcctgtc tcaaggccgg accggcaaga gccagacgtg
     2521 aaggtttccc ggtcatacgg tcaaagacct caatggcatc accaaccgca taaatatttt
     2581 tatcacttgt gcggtagttg tgatccacca aaatgccgcc ggtctctccg atctccagtc
     2641 cggctgcttt cgcaagcccc gtctccggcg ctacaccaat agccagaata accgcatccg
     2701 ctggcaccgt cttgccggaa gcaagggtaa tctgcttctc ttcaatggtt tttactccat
     2761 catccacaat taaagacacg ccgttgtcca gcagttcctt gtgaagcatc tgtaccatat
     2821 cgtaatcaaa gggcgccaga atctggctca tggcctccac cacagtaacc ttcttccccg
     2881 cctttgcaag gttttcagcc acctcaattc cgataaagcc accgccgata accgctgtgt
     2941 tctccgctcc tgcggcctcc agtccatcct ttaagtgaac aatatctgcc acattacgga
     3001 ctgtatacac attttttccg tcaattccgc cgatgcttct tggctttaca ggtgacgcgc
     3061 cgggagccat aaccaaaaca tcgtaggcct cctcatgtat gtccccggtc agcgtgtttt
     3121 taatctcaat ggtctttttg tcacggttaa tagccatcac ttcgctgtta acccgtgcgt
     3181 ctatatcgta agactttttg aatttttccg gggacatcat taccagtgca tcactgtttc
     3241 ccactactcc tgacagataa tagggaagag cacagttgga aaaggacaca tgctggccac
     3301 gctcaaacat aatgatttct gctcccgcgt cgagacgtct tgctctggct gctaccgaag
     3361 cgccgcctgc cacgcctcct atgatcagaa tccttttatt catggaaaat cactcctcgt
     3421 ataatatctt ttagatacat aaatacatat attttggtta ataagttact aaataaatat
     3481 ttgttcaata ggcttagtaa agcccatgat
//
LOCUS       DBMDUA010000252         3497 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9429_length_3497_cov_2.240872, whole genome shotgun sequence.
ACCESSION   DBMDUA010000252 DBMDUA010000000
VERSION     DBMDUA010000252.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9429_length_3497_cov_2.240872"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="ACZPCL_12850"
     CDS             complement(<1..257)
                     /locus_tag="ACZPCL_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HIR1163392.1"
                     /translation="MLEGRPLKGYDLERLKEFLKSMDLDYDMGIEYSICLEDEDYRII
                     AAGSVEENVLKCIAISPDHQGEGLSGTIISHLTQYEFEKGR"
     gene            complement(308..1648)
                     /locus_tag="ACZPCL_12855"
     CDS             complement(308..1648)
                     /locus_tag="ACZPCL_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1163393.1"
                     /translation="MTKDLTTGKIMPILVKFTIPLVLGNLFQLTYNAVDSIIVGHFVG
                     KEALAAVGICNPITTLMILFLNGLCMGAAILMGMQYGAKDYDTLHRQISTTMLSGVVF
                     SVTLTVLCVIFAVPMLKLIQVDPSIMDLTAEYLRIIFWGLMFTFLYNFFSSTLRALGD
                     SNSPLYFLIISAVLNVFGDLFFVIVLKDGSRGCAISTVLSEALCSLFCVIYIQKKVPL
                     LRLGKKWLVFDSRLLKKTIAFGWASAMQQATVQLGKIGIQAIVNTMGVSVSAAFAVVN
                     RIDDFAYTPEQNIAHAMTALMAQNKGAGKIDRMKEGFRCGIILETIYGILIFIICFAL
                     ARPLMMMFVKDEEVIGHGVTYLHLISVMYLLPALTNGIQGFFRGIGDLKITLISSFIN
                     MALRVLAAAPLVFIWKMGIEALPYSYLAGWIGMLVAETPLLLKTYKTWGKKAGQ"
     gene            complement(1891..3249)
                     /locus_tag="ACZPCL_12860"
     CDS             complement(1891..3249)
                     /locus_tag="ACZPCL_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0046942 - carboxylic acid transport
                     [Evidence IEA];
                     GO_process: GO:1905039 - carboxylic acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HIR1163394.1"
                     /translation="MSEVVVGLLGFACIVAIVVTLFKSKTLPSIAFIIFPMILGAILV
                     FGGYYSWENIGKLIKSGFSSTGPTAALFVFSVLYFGIMTDAGMFDVIIGKLMLLVKDN
                     VIGVCVMTCIIALIGHLDGGGASTFCIVVPAMLPVYKKMHMRPATLLRIAVISMGVLN
                     LMPWAGPTMRAATVLGIEAGSLWQTLLPIQACGIVLALVVAVLNGIIEQKRGAGLNGK
                     LAKEAAHLSVEEVAAADAENKDLARPKLFVFNVILTIAVIALLIKDIFPSYVPFMLGV
                     AIAILVNYPGAKMQKKIINLHAGPALMMCSTLMGAAVLMGILVKDIEGVNSVITCMSN
                     LISSILPAALGQHLPLVIGILSVPLALAFDTDSYFYGMLPVMIGIGEGFGVGAMPIAV
                     AMVVCRNCATFISPMVPATLLGVGLADVDIKDHIKFSFLWVWGFSIICMLIGAIVGII
                     PL"
     gene            complement(3374..3493)
                     /locus_tag="ACZPCL_12865"
     CDS             complement(3374..3493)
                     /locus_tag="ACZPCL_12865"
                     /inference="COORDINATES: protein motif:HMM:NF012698.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1163395.1"
                     /translation="MDDNAIMVYINRLRSKIEKDRQKPEHIITVRGMGYRFLP"
BASE COUNT         1042 a          830 c          679 g          946 t
ORIGIN      
        1 cttctgccct tttcaaactc atactgggtc aggtgggaaa taatcgtgcc ggaaagtcct
       61 tctccctgat gatccgggga aatggcgata cattttaata cattttcctc tacagaacca
      121 gctgcaatga tgcggtaatc ctcatcttcc agacagatac tgtactcgat ccccatatca
      181 taatccaggt ccatggactt taaaaattct ttcaaacgct ccaggtcata tccctttaat
      241 ggtcttcctt ctaacacgaa ttttctctcc cttcatataa tctgcattcc tgcaggactg
      301 cccagctcta ctgcccagct ttctttcccc aggtcttata agtctttaaa agtaatggtg
      361 tttctgcaac cagcattccg atccagccag ccagatagga atatggcagt gcttcgattc
      421 ccattttcca tataaatact aaaggcgcag ctgccagaac acgcagcgcc atgttgataa
      481 agctgcttat taatgtgatc ttcagatctc cgatgccacg gaaaaatccc tggataccat
      541 tggtaagagc cggaagcagg tacattacag aaatcaggtg cagataagtt acaccatgtc
      601 caataacctc ttcatctttt acaaacatca tcataagagg tcttgcaagc gcaaaacaga
      661 tgataaagat caggataccg taaatggtct ccagtatgat gccgcagcgg aacccctctt
      721 tcatacggtc gatttttcct gcccccttat tctgggccat aagggcagtc atggcatgag
      781 caatgttctg ctccggtgta taagcaaaat cgtcaatacg gttgacaacc gcaaatgcgg
      841 ctgaaacaga tacacccatg gtatttacga tcgcctggat accgattttt cccaactgca
      901 cagttgcctg ctgcatggca gatgcccagc caaaggcaat ggtcttcttt aaaagacggc
      961 tgtcaaatac cagccatttt tttccaagac gcagaagagg cacttttttc tggatataga
     1021 tcacacaaaa caggctgcat aaagcttcac ttaataccgt ggaaatagca cagcccctgc
     1081 ttccgtcctt taaaacgata acgaaaaaca gatcgccaaa tacatttaac acggcactga
     1141 tgatcaggaa atacagcggg gaattactgt ctcccaatgc ccttaaggta ctggaaaaga
     1201 agttatataa aaatgtaaac atcagtcccc agaatatgat ccgcaggtat tctgctgtca
     1261 gatccataat agacggatct acctggatca gttttaacat aggcactgca aaaataacac
     1321 agagcacggt caatgtaaca gaaaagacca ctcctgaaag catggtggtg ctgatctggc
     1381 ggtgcagggt atcataatct tttgctccat actgcattcc cattaaaata gcagctccca
     1441 tgcacagacc atttaagaat aagatcataa gtgttgtgat cgggttgcag ataccaaccg
     1501 ctgccagtgc ctcttttcct acaaaatgtc ctacaataat gctgtctact gcattgtacg
     1561 tcagctggaa cagatttccc aaaaccagtg gaatggtaaa tttcaccaga ataggcatga
     1621 tctttccagt tgtcagatct tttgtcataa tcctttatct ccttgctatt tcacgatctc
     1681 ttcgtgaaat ctgacctggt tttaatatta ggcagatttg aacggaaagt caatacattt
     1741 aaaatatgca aacagaccac cagtgcgttc ctgatatgca ttataaacaa aggagctgcc
     1801 gcaacgatac gcctgcagca gctcctttat ggggaaggat ttgaatttta aatttatcta
     1861 tctgatttta tgtaatgatc tgttatagga ttataatgga ataattccta caattgctcc
     1921 aataagcata cagatgatac tgaagcccca tacccacagg aagctgaatt taatatggtc
     1981 tttgatatct acatctgcca gacctacacc taataaagtt gcaggtacca ttgggctgat
     2041 aaaggttgca cagttacggc atacaaccat ggcaactgcg atcggcattg ctccaacgcc
     2101 aaatccttcg ccaattccga tcataacagg aagcattccg tagaagtagc tgtctgtatc
     2161 gaaagccagt gctaacggta cacttaagat accaattact aatggcagat gctgtccaag
     2221 tgctgccggt aaaatgctgc tgatcaggtt tgacatacag gtgataacac tgtttacacc
     2281 ttcaatgtcc ttaaccagga tacccattaa tacagctgca cccattaagg tagagcacat
     2341 catcagagcc ggacctgcat gaaggttgat gatctttttc tgcatctttg cgcctgggta
     2401 gtttaccaga attgcaattg caacacccag catgaaagga acatagcttg gaaatatgtc
     2461 tttaattaac agtgcgataa ctgcaatggt taaaataacg ttgaatacaa acagttttgg
     2521 acgagccaga tctttatttt ccgcatcagc tgcagctact tcttctacgc tcagatgagc
     2581 tgcttctttc gccagcttac catttaaacc agcgcctctc ttctgctcaa tgataccgtt
     2641 taatactgca acaaccagtg ccagtacgat accacatgcc tggatcggta aaagagtctg
     2701 ccataaggaa cctgcctcaa taccaagaac ggttgctgct ctcatagtag gaccagccca
     2761 tggcatcagg tttaaaacac ccatggaaat aactgcaata cgaagtaatg ttgccggacg
     2821 catatgcatc ttcttgtaaa caggaagcat agccggaact acgatacaga aagtggaagc
     2881 gccgccgcca tccagatgcc cgatcagggc aatgatacag gtcatgacac agacgccaat
     2941 gacattgtcc ttaactaaca gcattaactt tccgataatt acatcaaaca ttcctgcatc
     3001 ggtcatgata ccgaagtaca gaacgctgaa tacgaacagt gccgctgttg gacctgtgct
     3061 ggaaaaaccg cttttgatca gcttaccaat gttttcccat gaatagtaac cgccaaatac
     3121 aagaattgct cccaggatca tcgggaagat gataaatgcg atgctgggaa gggttttgct
     3181 cttaaaaagt gttacaacga tagcaacgat acaggcaaaa ccaagaagac ctactacaac
     3241 ctctgacata ctctttctct cctttacatt tttatgtcat gtttctatct gtaaatgagt
     3301 atatcgaggc aaccttacag gtataagaga tttatcttac aatttgttaa ggtttgtatt
     3361 aatcttcgta catttacggg aggaaccgat atcccatccc gcgtaccgta ataatatgct
     3421 ctggtttctg cctgtctttt tcgatcttgc tgcgcagacg gttgatatag accataatgg
     3481 cattgtcatc aatggcg
//
LOCUS       DBMDUA010000253         3483 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9476_length_3483_cov_2.212005, whole genome shotgun sequence.
ACCESSION   DBMDUA010000253 DBMDUA010000000
VERSION     DBMDUA010000253.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9476_length_3483_cov_2.212005"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            150..1310
                     /locus_tag="ACZPCL_12870"
     CDS             150..1310
                     /locus_tag="ACZPCL_12870"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1163396.1"
                     /translation="MKTTSSFLDLPQSGIRRMYDLAKNKKDTVSFVLGEPDFVTPKHI
                     IEAAKKKLDEGCTHYTDNAGILPLRQEISRALKQYDKVDYDPEGEIVVTVGGMMGMYM
                     AILALVNPGDEILIADPSYTNYVGEIVMNRAVAVPVPVYEKDNFNFTYENLKSRVTDK
                     TKAIILNSPCNPTGGVATRETMETVAKVALEYDLYVIYDAVYKHLIYNDTDYINIAVL
                     DGMRERTIYVDSFSKTYAMTGWRLGYMAGPRNILSRLPKLQENMPSCLPEFVQYAGIE
                     ALKNGDEDIAMMNRQYAERRKLVLERINGIKGLSCTPPNGAFYAFVNIKETGMTSVEF
                     CEKLLEKEGVVTAPGSAFGEQGEGYVRLSYATSMEQINRGMDRIQRFMESIM"
     gene            1767..3134
                     /locus_tag="ACZPCL_12875"
     CDS             1767..3134
                     /locus_tag="ACZPCL_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1163397.1"
                     /translation="MAESNKMKDMPVNKLMIQMGIPMILSMALQAVYNIVDSAFVGNM
                     RVGSEAALNALTLVFPVQMLMVAVGIGTGVGTNALLARTLGQGNSKKAAKVAGNSLFL
                     GVIIYVVCLLFGIFGVRAYISSQTVDAEVLEMGVSYLRICCVISFGIIFFSLFEKLLQ
                     ATGRSLYSTIGQVVGAVVNIILDPVMIYGIGPCPEMGVEGAAYATVIGQVASAVLLLI
                     FHIRLNKEFGHGVKYMKPDAGVIKEIYAIGLPAIIAQALMSIMVYVMNLILKFNPSAQ
                     TAYGLFYKVQQFVLFLAFGLRDAITPIIAFAYGMRNKKRIQDGIKYGLIYTIALMILG
                     IGITEIFPGAFATLFNAGQSREYFIGAMRVISVSFLFAGINVAYQGIYQALDGGLESL
                     VISLLRQLIIILPLAGIFSLFVRNGQMGISLIWWAFPVTEVIACLAGYVFLKKIRKNR
                     VNTLI"
     gene            3148..>3483
                     /locus_tag="ACZPCL_12880"
     CDS             3148..>3483
                     /locus_tag="ACZPCL_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005347938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HIR1163398.1"
                     /translation="MAKKIITISREFGSGGRFIGEEVAKKLGIAYYDKNIINHIAEES
                     GLSPDYIQEKAELSPKKGLFAYALVGRDITGKSVEDMVYERNEGVSAARNKGIELSRG
                     GYLFFIDSDD"
BASE COUNT         1074 a          545 c          848 g         1016 t
ORIGIN      
        1 aaataagaat ggtgaatttt gttcaactta atatgaatat atagcaattc cattcacaac
       61 aagactatgt tacagttaag aagtcgatag aaaaacgaaa aggtatactt gctcgagaag
      121 gtatttctaa caaacggagg ggagacaaaa tgaagacaac aagcagtttt ttagatctgc
      181 cgcagtcagg aatcagaaga atgtatgacc tggcaaaaaa taaaaaagat actgtaagtt
      241 ttgttcttgg tgagccagat tttgtgacac caaagcacat tattgaagct gcaaagaaaa
      301 aactggatga agggtgtacc cattatacgg ataatgcagg cattttgccg ctgcgccagg
      361 aaatttccag agctttaaaa cagtatgaca aggtagatta cgatccagaa ggtgagatcg
      421 tagtgacggt aggcggaatg atgggaatgt atatggcgat tcttgccctt gttaatcctg
      481 gggatgagat cctgatcgca gatccgtctt ataccaatta tgtaggtgag attgtgatga
      541 accgtgcagt tgcagtacct gtacctgtgt atgaaaagga taattttaac tttacttatg
      601 aaaatcttaa aagtagagtg acagataaaa ccaaagccat tatccttaat tctccctgca
      661 atcctacggg gggcgttgca acaagggaaa ccatggagac cgttgcaaaa gtagctttag
      721 aatatgatct ttatgtgatc tatgatgctg tatataaaca tcttatctat aatgatacag
      781 attatattaa tattgcagtt ttagatggaa tgagagaacg cactatttat gtagattctt
      841 tttctaaaac atatgccatg acaggctggc gtcttggata tatggcggga ccaagaaata
      901 tcctttccag actgccaaag ttgcaggaaa atatgccttc ctgtcttcct gagtttgtac
      961 agtatgcagg aattgaagcg ttaaaaaatg gcgatgaaga tattgcaatg atgaacagac
     1021 agtatgcaga aagaagaaaa cttgttctgg agcggatcaa tggcatcaaa ggtttatcct
     1081 gtacaccgcc aaacggagcc ttttatgcat ttgtaaatat taaagaaaca gggatgactt
     1141 ctgtggaatt ttgcgagaaa cttctggaga aagaaggcgt tgtaacagct cccggatcag
     1201 catttggaga acagggcgaa ggatatgtgc gtctttccta tgcaacatcc atggagcaga
     1261 ttaaccgggg gatggaccgg atccagcggt ttatggaaag tattatgtaa atatacaggt
     1321 aaatgcctga atttcaccag accgcctgat cgttatgggg gagtttgccg gtagtgctta
     1381 tccttatagg cggtggcatg cggtatttct gcttataata tgtttgaaaa gactggtatg
     1441 tggatcgtgc caaattttat tatataaatc cgatgggatg acttacagtg atatcgtttt
     1501 ccttgtggta tattaggaca ttatcataaa tggttcataa aagagaaaat gccagaaaaa
     1561 ttttgtttga ctttttgaaa aacaggaaat ataattacaa cgaacgagta gcaggtgagc
     1621 gtaaatccag tttacatgct gctcgttttt attttattaa aatagaatat ttgccatgaa
     1681 aaagtgtgta gcttgtcagc gtaagtccag cttatatgac aaggtgcgca ctttttttgt
     1741 gccaaaaaga gaggagttaa aacaaaatgg cagaaagtaa caagatgaaa gatatgccgg
     1801 tgaataagct gatgatccag atggggatcc ctatgatctt atccatggca ttgcaggcgg
     1861 tctataacat tgtagacagt gcatttgtag gaaatatgag agtgggcagt gaagcagcat
     1921 taaatgcact gacattggta tttccggtgc agatgcttat ggtggcagtg gggatcggaa
     1981 caggtgtagg aacaaatgcg cttcttgcaa ggacactggg acaggggaac agtaaaaagg
     2041 ctgcaaaggt agcaggaaac agcctgtttc ttggtgtgat catttatgtg gtatgtttat
     2101 tgtttggcat ctttggtgtg agagcatata tttcatcaca gacagttgat gcagaagtat
     2161 tggaaatggg agtcagttat ctgaggatat gctgcgtgat ctcttttggt attattttct
     2221 tttctttatt tgaaaagtta ttacaggcaa cgggccgttc cctttattca acaattggcc
     2281 aggtagttgg agctgttgta aatattatcc ttgatccggt catgatctat ggtattggac
     2341 cttgtcccga aatgggagtg gaaggtgcgg cctacgcaac tgtaattggc caggtagcat
     2401 cagcagtatt gctgcttata tttcatataa ggctgaataa ggaattcggg catggagtga
     2461 aatacatgaa gccggatgcc ggtgttataa aagaaatcta tgcaatcgga cttcctgcaa
     2521 ttattgcgca ggcattgatg tccattatgg tttatgtgat gaatctgatc ctgaaattca
     2581 atccgtcagc acagacagct tatggattgt tctataaggt tcagcagttc gttctcttcc
     2641 ttgcctttgg cctgagggat gcgatcactc ccatcattgc atttgcatat ggaatgagaa
     2701 ataaaaagag aattcaggat gggatcaaat atggcctgat ctatactatt gcattaatga
     2761 tcctgggaat tgggatcaca gagattttcc ctggggcatt tgcaactttg tttaatgcag
     2821 gacagtcaag agaatatttt atcggtgcaa tgagagtgat ctctgtcagc tttctgtttg
     2881 ccggaattaa cgttgcttat caggggattt atcaggcatt agatggcggc ttggaatcac
     2941 tggttatttc acttttgaga cagcttatta tcatattgcc attggcggga atcttttcat
     3001 tatttgtcag aaatggccag atgggaattt cactgatctg gtgggcattt ccggtcactg
     3061 aagtgatcgc atgtctggct ggatatgtat ttttaaagaa aatcagaaaa aacagagtta
     3121 atactttaat ttaaggagga actggaaatg gcaaaaaaaa tcattacgat cagcagggaa
     3181 tttggaagcg gtggacgttt tatcggtgaa gaagtagcaa agaaactggg aatagcctat
     3241 tatgataaaa acatcattaa tcatattgca gaagagtcag gtctgtctcc ggattatatc
     3301 caggaaaaag cagagctgtc tccgaagaaa ggattatttg catatgcact tgtaggacgt
     3361 gatattacag gaaaatcagt ggaagatatg gtgtatgaga ggaatgaagg agtctctgcg
     3421 gcaaggaaca agggaataga actatcccgg ggtggttatc ttttttttat agattcggat
     3481 gat
//
LOCUS       DBMDUA010000254         3442 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9574_length_3442_cov_1.991619, whole genome shotgun sequence.
ACCESSION   DBMDUA010000254 DBMDUA010000000
VERSION     DBMDUA010000254.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9574_length_3442_cov_1.991619"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="ACZPCL_12885"
     CDS             complement(<1..557)
                     /locus_tag="ACZPCL_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1163399.1"
                     /translation="MERGPVRVAMMADTHGVLRPEVEKILETCDVIVHAGDFDTQMLY
                     MKLSGKQPLFAVRGNNDRGWSGGLPGIKRFEIGGVKMVMAHQRTDIPVALGDAQVVIF
                     GHSHMYQQQEIAGRLWLNPGSCGYKRNTLPLSMAVMTIEDGTYQVETIWLEKGYGTPG
                     AAIAQREKTKVSKYEKKQKRYQQKQ"
     gene            793..2256
                     /locus_tag="ACZPCL_12890"
     CDS             793..2256
                     /locus_tag="ACZPCL_12890"
                     /inference="COORDINATES: protein motif:HMM:NF039231.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR1163400.1"
                     /translation="MIKNYKKLFLSFGAAAVLSSAAAFSSLAASGGWVSENGVWKYQE
                     ASGNYAYNTWKTSGDNSYYLDNNGVIAVNQWIDDTYYVDDDGVMAKNSWIQLSEDTSQ
                     KKAGWYYVDAKGKLIKDKWSTIGDKKYAFDSDGKMRTGWFFDNDDIYYLGDNGYAKTG
                     WLCLDYDEDNKPDDGTISEIRTSASDTAKWFYFQTNGKAKRSKNDSYSAETIGDNKYY
                     FNEDGVMMTGWVAVKSSAEAGDPTGISKFVYLGGDDEGIMAKNKWLELYNHPGDSDDK
                     DEITTDNSDEAPDEGESNWYYFESDGTPAYLNSKATNMSKATTKVDGNSYFFNSYGVR
                     QTGLIHVVTSSGKEFTGYFGENDSDGKMLTGKKSNLSVDGETAAYYFSESGSDKGCGY
                     NGSKDDYLYYQGRLVKADSGSDYQVFCVDNKMYLVNESGKIQTSSKNYKVNGSWTFKV
                     SGGSIYFIDDDKKSTGKVTSSDAESLPEVIYDKEYTL"
     gene            complement(2330..>3442)
                     /locus_tag="ACZPCL_12895"
     CDS             complement(2330..>3442)
                     /locus_tag="ACZPCL_12895"
                     /inference="COORDINATES: protein motif:HMM:NF013126.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR1163401.1"
                     /translation="VPENRAWHEANRTYTFSYGLGADYDDPKWDEVLNQLTEKETEEL
                     FLHGYVKTEELENIGKPLAREADGPAQIGSFNQNPTGTGFPNAGVMAQSWNTELSRDM
                     GRAVAKEAGQFGYSGWYAPAVNIHRSPFDGRNYEYYSEDSFLSGKMCGAAVSGSMDMG
                     VYCYVKHLICNDQESGIYRDGVYTWMTEQALRETYLKPFQMIVQDNGATGLMSSYNRI
                     GAVWAGGSKGLLTSILRDEWGFKGAVLTDYSDHHKYMNGDQALAAGGTLWMDGFMNDG
                     SFAFKNEENKAAYDQALRQSAKDVIYMYLNARVRNQAYAEEMDDWSLLKPEIKAPFPL
                     WAAGLAAVDLLAIGNFVFAVNIWKKRKRELLKDHEN"
BASE COUNT          928 a          811 c          706 g          997 t
ORIGIN      
        1 aactgttttt gctggtaacg cttctgcttt ttctcatatt tacttacctt tgtcttttca
       61 cgctgggcaa tggcagctcc cggagtaccg tatcccttct ctaaccatat ggtttctacc
      121 tgatacgttc cgtcttcaat ggtcataaca gccatggaaa gaggcagtgt attccgctta
      181 taaccacaac ttccgggatt tagccacaac cttcccgcga tctcctgctg ctggtacata
      241 tgggaatggc caaagatcac tacctgagca tctcctaatg ccacagggat gtccgttctc
      301 tgatgggcca tgaccatttt cacaccgccg atttcaaaac gtttgatccc cggcagtccc
      361 ccggaccagc ctctgtcatt atttcccctt actgcaaaaa gcggctgctt tccgcttaat
      421 ttcatataca gcatctgcgt atcaaaatca cctgcatgta cgatcacgtc gcaggtttcc
      481 agtatcttct ccacctccgg gcgcagcact ccatgggtat ctgccatcat tgccaccctt
      541 acaggtcccc gttccatttt tcttcctcct tggtttcaca tatacacgct ctgaaaatac
      601 cagattatta tatacgatta ttttctgact gacaatcatt tcagccataa catcttcttg
      661 tattatttat tttttctgac gattttgtga acattattgt ggaaaaaata gttagatgtt
      721 atacttgcag cagaccattg tacattttga tataaatata cggatctgta catacatgaa
      781 aggggaaact gtatgataaa aaattataaa aaactattcc tgtcctttgg agctgcagct
      841 gttttaagtt ccgcagctgc ttttagcagc ctggctgcaa gtggcggctg ggtcagtgaa
      901 aacggagtat ggaaatatca ggaagcatct ggaaattatg cctacaatac ctggaaaact
      961 tccggtgata actcttacta tctggacaat aacggcgtca ttgctgtaaa ccagtggatc
     1021 gatgatacct actacgttga cgatgacggc gtaatggcaa aaaattcctg gatccagtta
     1081 tcagaagata cttcccagaa gaaagcaggc tggtattacg tggacgccaa aggaaagctg
     1141 atcaaggata aatggagtac catcggggat aaaaaatacg cttttgacag cgatggaaaa
     1201 atgcgtacag gctggttttt tgataatgat gatatttatt atctgggaga caatggttat
     1261 gccaagaccg gctggctgtg cctggactat gacgaagaca acaagccgga tgatggaacg
     1321 atctctgaaa tccgtacttc tgcttccgat actgccaaat ggttttattt ccagacaaac
     1381 ggaaaggcaa aacgttctaa gaatgactcc tattccgctg aaaccatcgg ggataataaa
     1441 tattatttta atgaagacgg tgtgatgatg actggctggg tagctgtaaa aagcagcgct
     1501 gaagcaggag atcccacagg tatcagcaaa ttcgtctatt taggcggaga tgatgaaggc
     1561 atcatggcaa aaaacaaatg gctggaactt tacaatcatc caggtgactc tgatgacaag
     1621 gacgagatca ccacagacaa cagcgatgaa gccccagacg aaggtgagtc aaactggtac
     1681 tactttgaaa gtgatggtac ccctgcttac ttaaacagca aagctaccaa tatgagcaag
     1741 gcaactacca aggtagatgg aaattcctat ttttttaact cctatggtgt ccgccagacc
     1801 ggccttatcc atgtggtaac ttcttccggt aaagaattta caggctactt tggtgaaaat
     1861 gactctgacg gtaaaatgct caccggtaaa aaatccaatt taagcgttga cggtgaaaca
     1921 gctgcttact atttcagtga gtccggctct gataagggct gcggttataa tggttccaaa
     1981 gatgattatc tgtattatca gggacgcctt gtaaaggcag acagcggttc tgactatcag
     2041 gtattctgtg ttgacaataa aatgtacctg gttaatgagt ccggcaagat ccagacttcc
     2101 agcaagaatt ataaagtaaa cggatcctgg acattcaagg tttccggcgg ttctatttat
     2161 ttcattgatg atgataagaa atccacagga aaggttactt cttctgatgc ggagtccctt
     2221 ccggaagtga tctatgataa agagtatact ttataacgct ttataatact acaggcttcg
     2281 gcattggttt aatcctttgc cgaagccttt ttgtatgaaa acatattttt taattttcat
     2341 ggtcttttaa aagttctctt tttcgtttct tccagatatt tacagcaaac acaaaatttc
     2401 caatagccag aaggtctact gccgccagtc ccgctgccca caatgggaac ggtgccttga
     2461 tctccggttt taacaagctc cagtcatcca tttcttctgc atacgcctgg tttctcactc
     2521 tcgcgtttaa atacatgtag atcacatctt ttgctgactg ccgcagtgcc tgatcatagg
     2581 ctgccttatt ttcttcattt ttaaaggcaa aactgccatc attcataaaa ccgtccatcc
     2641 aaagggttcc ccctgctgca agagcctgat ccccgttcat atatttatga tggtcagaat
     2701 aatctgtaag cacggctcct ttaaagcccc attcatccct taaaatgctg gtaagaagcc
     2761 ccttactgcc tcctgcccac acagctccta tacggttata ggaggacata aggccggtgg
     2821 caccattatc ctgtacgatc atctggaacg gctttaaata agtttcccgc agggcctgtt
     2881 ccgtcatcca ggtatagacg ccatccctgt aaataccaga ctcctggtca ttgcagatca
     2941 gatgttttac atagcagtat acacccatat ccatacttcc acttaccgct gccccgcaca
     3001 ttttcccgga aagaaagctg tcttctgaat aatattcata gtttcggcca tcaaaaggac
     3061 tgcggtggat attgaccgcc ggtgcatacc agccgctata gccaaactga cctgcttcct
     3121 ttgcgactgc ccgtcccata tcacgggata attctgtatt ccatgactgg gccataacac
     3181 cggcattggg aaatccggtt cctgttggat tttggttgaa actaccgatc tgtgccggtc
     3241 cgtcagcttc tctggctaat ggttttccaa tattttccag ttcttctgtc tttacatacc
     3301 cgtgaaggaa cagctcctct gtctcttttt ctgtcagctg gtttaaaact tcatcccact
     3361 ttggatcgtc ataatccgct cccagtccgt aagagaaagt ataagtgcgg tttgcttcgt
     3421 gccatgcacg attttccggg ac
//
LOCUS       DBMDUA010000255         3417 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9660_length_3417_cov_3.014475, whole genome shotgun sequence.
ACCESSION   DBMDUA010000255 DBMDUA010000000
VERSION     DBMDUA010000255.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9660_length_3417_cov_3.014475"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(114..>770)
                     /locus_tag="ACZPCL_12900"
     CDS             complement(114..>770)
                     /locus_tag="ACZPCL_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163402.1"
                     /translation="LVAACCAGVATFGMFVASKFNLTTSRNQVPLTSVKIEGEKVLIP
                     ENGKTTREAGWTLRNSRGQYILTLDELEAGSLDTAEPVIEVEGDLILQLKKDTISHLE
                     SQGPVIKKGTGTLTIRGEGSLELESADAEAISSDWNGEELDADHPSAVRLEAGNLTFT
                     GAGSGIVDETVELAGAQGTITATSTSGAAVKTAHVKREGSNSDKLELIGATAVAVPES
                     "
     assembly_gap    771..780
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(827..1498)
                     /locus_tag="ACZPCL_12905"
     CDS             complement(827..1498)
                     /locus_tag="ACZPCL_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIR1163403.1"
                     /translation="MEREIDLKEISDGRLYSSSDMVKADCGDCKGCSACCQGMGSSIL
                     LDPYDIYRLSTGTGLTFEDLLKGRIELNMSDGLILPNLKMAGEKEACSFLNGEGRCSV
                     HPFRPGFCRLFPLGRLYENGSFQYFLQTHECKKENRTKVKVRKWIDIPDFGKYEKFVS
                     DWHYFAKEMGQKIKSLGSEGQDKSKALCLYVLKKFYMEPFDASVDFYEQFNKRLEEAK
                     HAAAA"
     gene            complement(1578..2342)
                     /locus_tag="ACZPCL_12910"
     CDS             complement(1578..2342)
                     /locus_tag="ACZPCL_12910"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR1163404.1"
                     /translation="MKYRRLLLGGFLAMGLAVTTASGALAEQIVAVNSPVESVGGQAT
                     KVTDAGQNAADTSQATVNSSVNTDTTQSAAGNTQTDTAQNTEASAAKPDPASITPTAT
                     GISIYISKRQSKLTLMQNKQAIGVWDAKLGRQSATGDKVQEGDEITPSGSFYVCTRND
                     KSKYYLALGLSYPNIEDAERGLSTGLITQEQYQAIVDANKAGVTPPWDTPLGGAIEIH
                     GNQGDRGTAGCIAMTNDVMDILWSYCAVGVPVTIGP"
     gene            complement(2497..>3417)
                     /locus_tag="ACZPCL_12915"
     CDS             complement(2497..>3417)
                     /locus_tag="ACZPCL_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HIR1163405.1"
                     /translation="EEENKIYGSVTAEEIEDRLKGKMQNKLKLEAALAQAVETLNVLN
                     AQSESLRSAIETLKSQIGSMQETSEGEIQEKITVLSEEKKEWNEKYSRQYADLENNRC
                     IYNNVQKNQGKMAAAEQEYVWMRSLSDTAGGTLSGKRKIELETYVQMAYFDRILRRAN
                     LRLMTMSSGQYELKRQEKGESKKEKAGLDLNVIDHYNGTERSVKTLSGGESFQASLSL
                     ALGLSDEIQAGAGGIRLDAMFVDEGFGSLDEDSLNQAIRSLNDLAQGQRLVGIISHVG
                     ELKDRIERKIIVTKKRSSTGVGSQVQVVGE"
BASE COUNT          785 a          915 c          646 g         1061 t
ORIGIN      
        1 ttgtgggctg tcttttcttc ttgccataaa aataacctcc aaactgagta attttatctt
       61 acatcagttt ggaggtttac acaaacttta ggatactccc gatattcctg actttatgac
      121 tctggcactg caactgctgt agctccgatc aactccagct tatcactgtt gctgccttct
      181 ctttttacat gagccgtttt aacagcagca ccggatgtag atgtagccgt aatcgtcccc
      241 tgggcgcctg ccagttctac tgtctcatcc acaataccag atcctgcccc tgtaaaagta
      301 agatttccgg cttccagacg gacagcggag gggtgatctg catccagctc ttccccattc
      361 caatcactgc tgattgcttc cgcatctgct gactccagtt ccagacttcc ttctccccgg
      421 atggtcagtg tacctgtgcc ctttttgatc accggtccct ggctttccag gtggcttatg
      481 gtatcttttt taagctgaag gatcagatct ccttctactt cgatcaccgg ttctgctgta
      541 tccagacttc cggcttccag ttcgtccaag gtcaaaatat actggcctct gctgttgcgc
      601 agcgtccagc ctgcttccct ggtggtcttt ccattttcag ggatcagcac cttttcgcct
      661 tcgatcttaa cagaagtaag aggcacctga tttctggagg tagtcagatt aaatttggat
      721 gccacaaaca ttccaaatgt agctactccc gcgcaacagg ctgccaccag nnnnnnnnnn
      781 cagaccgata aacagaacag tacggaccat tcttccaaca aattctttag gcagctgctg
      841 catgttttgc ttcctccagt ctcttgttaa actgttcata aaaatccacg gaagcatcaa
      901 atggctccat gtagaacttt ttcagaacat acaggcacaa agcctttgac ttatcctggc
      961 cttcacttcc aaggcttttt atcttctgtc ccatttcttt tgcaaaataa tgccagtcag
     1021 aaacgaattt ttcatatttg ccaaaatccg ggatatcgat ccatttacgc acctttacct
     1081 tggtcctgtt ttccttctta cattcatggg tctgtaaaaa atactggaaa gaaccatttt
     1141 cataaagtct ccctaaaggg aacagacggc aaaatcccgg tctgaatgga tgaacggagc
     1201 atctgccttc tccatttaag aaagaacagg cttctttttc tcctgccatt ttcaggtttg
     1261 gcaggatcag accatctgac atattcagtt cgatccgacc ctttaacaga tcttcaaaag
     1321 tcagccctgt tcctgtactt aaacggtaaa tgtcataggg atccagtaaa atagaggatc
     1381 ccattccctg acaacaggca gagcagcctt tacaatctcc gcagtctgct tttaccatat
     1441 ccgaggaact gtacaatctt ccatcggata tttcttttaa atctatttcg cgttccatta
     1501 tttcttttgt ttcctttcac acgccgaaat ctttgacgat accggcaaga tttccggcta
     1561 ctgtaagtgt tacgggctta cggaccgatg gttaccggca cacccactgc acaataactc
     1621 cacagaatat ccattacatc attggtcatg gcaatacagc ctgccgttcc acgatctccc
     1681 tgatttccat ggatctcaat agctccgcct aaaggagtat cccatggtgg ggtcacgcct
     1741 gctttgtttg catctacaat cgcctggtac tgttcctggg taataagacc ggtggacaga
     1801 cctctttctg catcctcaat atttggatag gacagtccaa gtgccagata atacttgctc
     1861 ttgtcatttc tggtacatac atagaaactg ccggaagggg tgatctcatc cccctcctgc
     1921 actttatcac cggtagctga ctggcgtccc agttttgcat cccagacgcc aatggcctgt
     1981 ttattctgca tcagggtcag cttgctctga cgtttgctga tataaatgct gataccggta
     2041 gctgtcgggg tgatggatgc cggatctggt tttgcagcag aagcctctgt attctgggca
     2101 gtatctgtct gggtgttgcc tgctgcactc tgggtcgtat ctgtattgac tgaagagttg
     2161 actgttgcct gagaggtatc tgctgcattc tgacctgcat ctgtcacctt ggttgcctga
     2221 ccgcctacag actcaaccgg gctgtttaca gcaacgatct gctctgcaag cgcaccggaa
     2281 gcggttgtca cagccagtcc cattgccaga aaacctccaa gcagtaatct tctatatttc
     2341 atctattaca tctcctttgg gaaaacatat atatttgcat taaattttct atctgctccc
     2401 cattttacca gaacacagct tttggagcaa ccgcactttt tttaagattt tctgtcattt
     2461 ttccaatata tgtcatctat ttctaaccat attcccttac tctcctacca cctgcacctg
     2521 gcttccaacg ccagtactgc tgcgcttttt ggtaacaatg atcttacgtt cgatcctgtc
     2581 tttcagctct cctacatggg agataatacc taccagtctc tgtccctgcg ccaaatcatt
     2641 taaggaacgg atcgcctggt ttaaagagtc ctcatctaag gaaccaaagc cctcatctac
     2701 aaacatggca tccagacgga tcccccctgc tcccgcctgg atctcatcag aaagccctaa
     2761 tgcaagggac agggatgcct ggaaagactc accgccggaa agtgttttta cacttcgctc
     2821 tgttccgttg taatgatcga tgacatttaa gtccagacct gctttttctt tcttactctc
     2881 ccctttttcc tggcgtttta actcatactg accgctgctc atagtcataa gccgcagatt
     2941 agccctgcgc aggatccggt caaaataagc catctgtaca taggtttcca gctcgatctt
     3001 gcgctttccg ctgagagttc cccctgctgt atcagaaagt gaccgcatcc acacatattc
     3061 ctgctctgct gctgccattt ttccctgatt tttctgaaca ttgttgtaaa tacaacgatt
     3121 attttccaga tctgcatatt gtctggaata tttctcattc cattccttct tttcttctga
     3181 aagaacagtt atcttttcct ggatctcccc ttcggaagtt tcctgcatgg acccgatctg
     3241 ggactttaaa gtttctatgg cagatctcag actttcagac tgggcattta aaacatttaa
     3301 tgtttccact gcctgagcca gtgctgcttc cagtttcagt ttattctgca tttttccttt
     3361 cagccggtct tctatttctt ctgctgtaac agaaccatag atcttatttt cttcttc
//
LOCUS       DBMDUA010000256         3376 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9807_length_3376_cov_2.637252, whole genome shotgun sequence.
ACCESSION   DBMDUA010000256 DBMDUA010000000
VERSION     DBMDUA010000256.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9807_length_3376_cov_2.637252"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="ACZPCL_12920"
     CDS             <1..668
                     /locus_tag="ACZPCL_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1163406.1"
                     /translation="YEDGKPKRSDYRKFKIRTVQGPNDYASMEEVLTRRFSHGIQESE
                     NLREKGKDISYGSFTRFPDLIMMDGGRGQVNIALAVLDRLGLSIPVCGMVKDDNHRTR
                     GLYYNNVEIPIDRHSEGFRLITRIQDEAHRFAIEYHRSLRGKAQVRSVLDDIEGIGPT
                     RRKALMRHFKDIDTIRSATAEELEQVPQMNRKAAESVYLFFHEPEKIQEDNAQAGAGN
                     ENT"
     gene            771..1706
                     /gene="hprK"
                     /locus_tag="ACZPCL_12925"
     CDS             771..1706
                     /gene="hprK"
                     /locus_tag="ACZPCL_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR1163407.1"
                     /translation="MHGVTITELIEKMNLRNSTPQIDTDKIVLTHPDVNRPALQLTGF
                     FDHFDRERVQIIGYVEQAYIKTMERDVKRQMFDKLTSSQIPCLVFSRSQEPDDDLLEY
                     CNYYGVPCLVSDKTTSSLMAEVIRWLNVKLAPCISIHGVLLDVFGEGVLIMGESGIGK
                     SEAALELIKRGHRLVTDDVVEIRKVSDDTLIGSAPDITRHFIELRGIGIIDVKTLYGV
                     ESVKDTQSIDMVIKLEDWNKDKEYDRLGLEDQYTEFLGNKVVCHTIPIRPGRNLAIIV
                     ESAAVNYRQKKMGYNAAQELYNRVQANLKGMSPSK"
     gene            1749..2699
                     /gene="murB"
                     /locus_tag="ACZPCL_12930"
     CDS             1749..2699
                     /gene="murB"
                     /locus_tag="ACZPCL_12930"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR1163408.1"
                     /translation="MSEFCEKLEEKLKEVMTSERGNFRFMEPMKRHTTFRIGGPAAVF
                     ISPGSEEELREVLNLLKEENIPWTILGNGSNLLVSDEGFKGAVISMSEGWAYSGVLRE
                     DEKAGKTLIRAGAGELLSRTARLAMECSLTGMEFASGIPGTIGGALVMNAGAYGSEIC
                     NVLSRAKVMTTEGEILELPAEELELGYRTSCIPKKNYIVLEAVFELNKGSKEAISSQM
                     RELAGKRREKQPLEYPSAGSTFKRPEGYFAAKLIEDAGLKGFSVGGAMVSEKHSGFVI
                     NYNDATASDVMELCRQVREKVKALSGVELEMEVKRLGEFK"
     gene            2696..>3376
                     /gene="rapZ"
                     /locus_tag="ACZPCL_12935"
     CDS             2696..>3376
                     /gene="rapZ"
                     /locus_tag="ACZPCL_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR1163409.1"
                     /translation="MKLVIVTGMSGAGKTIALKMLEDIGFYCVDNLPISLVDKFVQLV
                     SEGTSIEKAALGLDIRSGEELGNLDEILENWRSSNVDVQVLFLDANDAVLIKRYKETR
                     RTHPLAGAGRLENGIEKEREKLAFLKREADYIIDTSMLLTRELRKELEKIFLQDARYK
                     NMYVTVLSFGFKYGIPEDADLVFDVRFLPNPYYDEHLRPLTGQVQAVRDYVMQGGTAP
                     WVLYFCGAV"
BASE COUNT         1021 a          664 c          896 g          795 t
ORIGIN      
        1 tctatgaaga cggaaagcca aagcgcagcg attaccgcaa gtttaagata cgtactgtcc
       61 agggccccaa tgattatgcg tccatggaag aggtgcttac acgccgtttc tcccatggaa
      121 tacaggaaag tgaaaactta cgtgaaaagg gaaaggatat ttcctatgga agttttaccc
      181 gctttccgga tctgatcatg atggatggcg gacggggaca ggtaaatatt gccctggcag
      241 tccttgatcg tctggggctt tcgatccctg tgtgcggtat ggtaaaggat gataaccaca
      301 ggaccagagg actttattat aataacgtag agatccccat tgaccgtcac agcgaaggct
      361 tccggctgat cacacggatc caggatgagg cccaccgctt tgccattgaa tatcacagaa
      421 gcctgcgggg aaaagcccag gtaaggtctg ttttagatga tatagagggc atcgggccaa
      481 ccaggagaaa agcccttatg cgccatttta aggatataga caccatacgc agtgccactg
      541 cagaagaatt agagcaggtg cctcagatga accggaaagc agcggagtct gtgtatcttt
      601 tctttcatga gccggaaaag atacaggagg acaatgccca ggctggtgcc ggaaacgaaa
      661 atacatgaca ataaccttaa aatgtgttag tataggaata aataattggt gaatattgga
      721 aaaatcaaaa gtacaataaa gtagaataat cacataaggg ggaaaccatt atgcatggag
      781 ttactattac agagctgatc gagaaaatga acctgcgaaa ttccacaccg cagatcgata
      841 cagacaagat cgtgctgacc cacccggatg taaaccgtcc ggcactgcag ctgaccggat
      901 ttttcgatca ttttgacaga gaacgtgtac agatcattgg atatgtagag caggcatata
      961 ttaagactat ggagcgtgat gtaaagcgtc agatgtttga taagcttact tccagccaga
     1021 ttccatgtct ggtattcagc agaagccagg agccggatga cgatctcctt gagtactgca
     1081 attactacgg cgttccatgt cttgtctctg ataagactac ttcttccctg atggcagagg
     1141 tgatccgctg gttaaatgta aagctggcac catgtatcag tatccacggt gtactccttg
     1201 acgtatttgg tgaaggtgtt ctgatcatgg gcgagagcgg tattggtaaa agcgaggcag
     1261 ctctggagct gatcaagcgc ggacaccgtc ttgttaccga tgacgtagta gagatccgca
     1321 aggtcagcga tgatacactg atcggaagcg caccggatat caccagacat tttattgaac
     1381 ttcgcggtat tggtattatc gacgtaaaga cactgtatgg tgttgaaagt gttaaggata
     1441 ctcagtctat tgatatggtc atcaaattag aagactggaa taaggataaa gaatatgacc
     1501 ggctgggact ggaagaccag tatacagaat ttttaggcaa caaggtagtc tgccatacca
     1561 tcccgatccg tccgggacgt aaccttgcca ttatcgtaga gtctgcagca gttaactacc
     1621 gtcagaagaa gatgggctac aatgcggcac aggaactgta caaccgtgta caggctaatt
     1681 taaagggaat gtctccgtca aaataggcag caaagaagag taaacgagta agagaggaat
     1741 aagaccccat gtcagagttt tgtgaaaagc tggaagaaaa gctgaaggaa gttatgactt
     1801 cagagagagg aaacttccgc tttatggagc ctatgaaacg gcacactact ttccgcattg
     1861 gaggaccggc agctgtattt atcagtcccg gttccgaaga agagctgaga gaggttctga
     1921 acctgttaaa agaagaaaat atcccctgga ccatcctggg aaatggaagc aacctgttag
     1981 tcagtgatga aggctttaag ggagctgtta tttccatgtc tgaaggctgg gcctattccg
     2041 gcgtattaag agaggatgag aaagcgggaa agacccttat aagagcagga gcaggagagc
     2101 ttttaagccg taccgcaaga ctggctatgg agtgttctct tacaggaatg gagtttgctt
     2161 ccggtattcc cggtaccatt ggcggtgccc tggtgatgaa tgcaggtgcc tatggctcag
     2221 agatctgtaa tgtcttatcc agagcaaagg ttatgacaac agagggtgag atcctggaac
     2281 ttcctgcaga ggaactggaa ctgggatacc gcaccagctg tatcccgaag aaaaattata
     2341 ttgtattaga agctgtgttt gaactgaaca agggaagcaa agaagccatc agcagccaga
     2401 tgcgggaact ggcaggaaaa cgcagggaaa aacagccctt agaataccca agtgcaggca
     2461 gtacctttaa gagaccagag ggatattttg ccgcaaagct gatcgaggat gccggtttaa
     2521 agggcttctc agtaggcggt gccatggttt ccgaaaagca cagcggtttt gtgatcaatt
     2581 acaatgatgc cacagcaagt gatgtaatgg aactttgcag acaggtaaga gaaaaagtca
     2641 aagccttaag cggggtcgag ctggaaatgg aagtaaaaag gctgggagaa ttcaaatgaa
     2701 acttgtgatc gtaaccggaa tgagcggggc aggaaagacc attgccttaa aaatgttgga
     2761 ggatatcggt ttttactgtg tggacaacct gccaatctct ctggtggata aatttgtaca
     2821 gcttgtatca gaaggtacca gtattgaaaa agcagcttta ggtctggata tacgaagcgg
     2881 ggaagagttg ggaaatctgg atgagatcct ggaaaactgg cgcagcagca atgtggatgt
     2941 acaggtactt ttcctggatg ccaatgacgc agtccttatt aaacgttata aagagactcg
     3001 ccgtacccat cctcttgcag gtgcgggaag actggaaaac ggcattgaaa aagaacggga
     3061 aaaactggca tttttaaaga gagaagcaga ttatatcatt gataccagca tgctccttac
     3121 cagggaactg cgaaaagaac tggaaaagat cttcttacag gatgccagat ataaaaacat
     3181 gtatgttacg gttctttctt tcggctttaa atacggaata ccggaagatg cagacctggt
     3241 atttgatgtg cgtttccttc caaatccata ttatgatgaa cacttacggc ctcttacagg
     3301 tcaggtgcag gcagtacggg actatgtgat gcagggcgga acagctccct gggtactcta
     3361 tttctgtggc gcagtc
//
LOCUS       DBMDUA010000257         3345 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9922_length_3345_cov_2.567201, whole genome shotgun sequence.
ACCESSION   DBMDUA010000257 DBMDUA010000000
VERSION     DBMDUA010000257.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9922_length_3345_cov_2.567201"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="ACZPCL_12940"
     CDS             <1..1185
                     /locus_tag="ACZPCL_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR1163410.1"
                     /translation="YFKGVVDKIEVEACVQFVDTFEENILGFCNNIFTQEGGTHLAGF
                     KTKFTALINSYARELGILKDKDSNFTGADTRNGMTAVIAVKHPDPIFEGQTKTKLASA
                     DATKAVFTVTGDQLQLYFDRNVEVLKGVIGCAEKSAKIRRAEEKAKTNMLTKSKFSFD
                     SNGKLANCESRDASKCEIFIVEGDSAGGSAKTARNRQYQAILPIRGKILNVEKASMDK
                     VLANAEIKTMINSFGCGFSEGYGNDFDITKLRYDKIILMTDADVDGSHIDTLLLTFLY
                     RFMPELIYDGHVYIAMPPLFKVIPKKGEEQYLYDEKDLERYRRNHTGDFTLQRYKGLG
                     EMDAEQLWETTLDPERRVLKRVEIEDARMASEVTEMLMGSEVGPRRQFIYEHADEAEI
                     DA"
     gene            1204..>3345
                     /locus_tag="ACZPCL_12945"
     CDS             1204..>3345
                     /locus_tag="ACZPCL_12945"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HIR1163411.1"
                     /translation="MAEKILRTEYSEEMQKSYMNYSMSVITARAIPDARDGLKPVQRR
                     VLYDMSQLHLDHDKPHRKSARIVGDTMGKYHPHGDSSIYDTLVVMSQEFKKGMALVDG
                     HGNFGSIEGDGAAAMRYTEARLEKFAQEVYLKDLDKTVNFVPNYDETEKEPEVLPVRV
                     PNLLVNGAEGIAVGMSTSIPTHNLGEVVDVVQAYIDDPAAPTEELMEYLPGPDFPTGG
                     IIANKSELPQIYETGVGKIKLRGRFEVELGKRKVDKDKLIITEIPYTMIGAGINKFLV
                     DVADLVESKKLTDVVDISNQSNKDGIRIVLELRKDADIDRIKNILYKKTKLEDTFGVN
                     MLAIADGRPETLNLKGILRNFMEFQYQNTERKYNVLLEKELDKKEIQEGLIAACDCID
                     LIIAILRGSKNLKDAKACLVNGDISNIHFKVAGFEEDAKKLHFTGRQASAILEMRLYK
                     LIGLEILALEKEHRETLKKIAEYKKILGSRAVMNQVIKDDLAAIKAEFAIPRRTRIED
                     GAEAVYVENEISVQEVVFVMDRFGYCKLLDKSTYERNQETVDTEQVHVLRCLNTDKIC
                     LFTSAGVLHQIKALDIPSGKLRDKGVPIENLSKYDGRNETICLLTTARELKGRILLFA
                     TRLAMVKQVPGEEFETNNRMVAATKLQEEDSVVSVTMINGETDVVLQTTNGTFLRFPL
                     EEISVLKKASRGVRGIRLAKNEELETVYLIGE"
BASE COUNT         1037 a          703 c          819 g          786 t
ORIGIN      
        1 tattttaagg gtgtagtaga caagatcgag gtagaagcct gcgttcagtt cgtagatacc
       61 tttgaggaaa atatccttgg cttctgcaac aacattttca cccaggaggg cggcacccat
      121 ttagctggat ttaagaccaa atttacagct ctgatcaact cttatgccag agagcttggc
      181 attttaaagg acaaagactc caactttaca ggtgccgata ccagaaacgg tatgacagcc
      241 gtgatcgctg taaagcaccc ggatcctatt tttgaaggac agaccaagac aaagcttgcc
      301 agtgcagatg ctaccaaggc tgtatttact gtaaccggtg accagctgca gctttacttt
      361 gaccgaaatg tagaagtgtt aaaaggcgtg attggctgtg cagaaaaatc tgcaaagatc
      421 cgcagggccg aagaaaaagc aaagaccaac atgctcacca agtccaagtt ttcctttgac
      481 agcaacggaa aactggcaaa ctgcgaaagc cgtgacgctt ctaagtgtga gatcttcatc
      541 gtagagggag actccgcggg cggttccgca aaaacagcca gaaaccgtca gtaccaggcc
      601 atccttccca tccgtggaaa gatcttaaac gtagaaaaag catctatgga taaggttctg
      661 gccaatgcag agatcaagac catgatcaat tctttcggtt gtggcttttc cgaaggctac
      721 ggaaacgatt ttgacatcac aaaactgcgc tatgacaaga tcatccttat gaccgatgcg
      781 gacgtagacg gaagccatat tgatacctta ttattgactt tcctttaccg tttcatgccg
      841 gagctgatct acgacggaca tgtatacatt gccatgccgc cgttatttaa agtgatcccg
      901 aaaaagggag aagaacagta tctctatgat gaaaaagatc tggaacgcta ccgcagaaac
      961 cacaccggtg attttacctt acagcgctac aaaggtcttg gtgaaatgga tgcagagcag
     1021 ctttgggaga ctacactgga cccggagcgg cgtgtattaa aacgggtaga gatcgaagat
     1081 gctagaatgg cgtcagaagt aacagaaatg cttatgggca gtgaggtagg accccgccgc
     1141 cagtttattt atgaacatgc agacgaagca gagatcgacg cataacagag aggacagaca
     1201 gatatggcgg agaagatatt aagaaccgaa tacagcgagg aaatgcagaa aagctacatg
     1261 aattattcca tgagcgttat cactgcaaga gcgatcccgg atgcaagaga cgggttaaag
     1321 ccagttcagc gccgtgttct ctatgacatg agccagcttc atctggacca tgataaacca
     1381 cacagaaaat cagcccgtat cgtaggcgat accatgggta aataccatcc acacggcgac
     1441 agctccattt atgatacctt agttgtcatg agccaggaat tcaaaaaagg aatggcattg
     1501 gtagacggcc acggaaactt cggctccatt gaaggggacg gggctgcggc tatgcgatat
     1561 acagaggcaa gactggagaa atttgcccag gaagtttatt taaaagacct ggacaagacc
     1621 gttaatttcg tgccaaacta cgatgaaaca gaaaaagagc cggaagtcct tcctgtacgt
     1681 gtgccaaacc ttttagtaaa cggcgcagag ggaattgccg ttggtatgag taccagcatt
     1741 cctacccaca atttagggga agtagtggat gtagtccagg catatattga tgatccggca
     1801 gctcctacag aagaactgat ggaatatctg ccgggacctg acttcccaac cggaggcatt
     1861 attgcaaata aaagcgagct gccacagatc tatgaaacag gtgttggcaa gatcaaatta
     1921 agaggccgtt ttgaggtaga actgggcaag cggaaagttg ataaggacaa actgatcatc
     1981 acagagattc cttataccat gatcggtgca ggtattaata aattcctggt agatgtggca
     2041 gatctggtag agtccaagaa gctgaccgat gtagtggaca tttccaacca gtccaataag
     2101 gacggtatcc gcattgtgtt agaacttcgc aaggacgcag atattgaccg tattaaaaat
     2161 attctttata agaagaccaa gttagaagat acttttggtg tcaacatgct tgctattgca
     2221 gacggcaggc cggaaacatt aaacttaaaa ggcatcctgc gtaatttcat ggaattccag
     2281 tatcagaata ccgagcgcaa atacaatgtg cttctggaaa aagaactgga taagaaagaa
     2341 atccaggaag gtctgatcgc agcctgtgac tgtattgacc tgatcattgc cattttacgt
     2401 ggttctaaga atttaaaaga tgccaaggca tgtctggtaa acggcgacat ttccaatatt
     2461 cattttaaag tagccggttt tgaagaagat gcaaagaagc ttcattttac agggcgtcag
     2521 gcatctgcga tcctggaaat gcgtctgtat aagctgatcg gattggaaat attagccctt
     2581 gaaaaagaac acagagagac tttaaagaag atcgccgaat acaagaagat ccttggcagc
     2641 agagcggtta tgaaccaggt gatcaaagat gatctggctg ccatcaaagc agaatttgct
     2701 attccaagac ggacccgtat tgaagacggg gcagaggctg tttatgtgga aaatgaaatt
     2761 tccgttcagg aagttgtttt cgttatggac cgcttcggct attgcaagct tcttgataaa
     2821 tctacctacg aacgcaacca ggagaccgta gatacagaac aggttcatgt gctccgctgc
     2881 ttaaatacag ataagatctg tctgttcacc tctgccggtg tccttcacca gatcaaggct
     2941 ttggatattc catccggcaa gctgcgtgac aaaggcgttc ctattgagaa cttaagtaaa
     3001 tacgatggca gaaatgagac catctgcctt ctcactacag cccgtgaact gaaaggccgc
     3061 atccttttat ttgcaacccg tcttgccatg gtaaaacagg tgcctggaga agaatttgag
     3121 accaacaacc gtatggtagc agctaccaag cttcaggaag aggacagtgt ggtttctgta
     3181 acaatgataa acggggaaac agatgtagtg cttcagacta caaatggaac gttcctgcgg
     3241 ttcccgttag aggagatctc tgttcttaag aaagcctcca gaggtgtccg tggtatccgc
     3301 ttagcaaaga atgaagaact ggaaacagta tatctcatcg gtgaa
//
LOCUS       DBMDUA010000258         3343 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9930_length_3343_cov_2.081431, whole genome shotgun sequence.
ACCESSION   DBMDUA010000258 DBMDUA010000000
VERSION     DBMDUA010000258.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9930_length_3343_cov_2.081431"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            27..1166
                     /locus_tag="ACZPCL_12950"
     CDS             27..1166
                     /locus_tag="ACZPCL_12950"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR1163412.1"
                     /translation="MENVWILGGLRSFIGVKDGIYRHIPAEVLGAQVLKEIKNRFLLK
                     DEEIDYIIGGNAVAGGGNITRLAALTAGLDEMIPAVTLDLQCGSALESITAAAAKIES
                     GLADLVIAGGFESSSTQPVRMWNSNHPDYQEGKSYTVAKFIPDIQGEQVMLEGAEKTA
                     LTENVTKAEMDSWVLLSHKRAAQAAKEGILSDIQLSIADSKKDEGIRSSMSQRLLDRL
                     PCLLPGGSIITAANACLMHDGAAFVVLCSEAYRKKHGLTPQAAFRFGTAIGSDPFMSP
                     KTAVLAIRKLLKQTALSIDEIHSLEVNEAFAVIDVLLEREFPGIRERLNPLGGALAYG
                     HPYGASGAIILLHLLKDLELNQGRFGICGVAAAGGIGTTLLVEKV"
     gene            1195..2493
                     /locus_tag="ACZPCL_12955"
     CDS             1195..2493
                     /locus_tag="ACZPCL_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012711.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR1163413.1"
                     /translation="MEKITNYLELIQQIQDITPEKEAFCTTGRSLTYSQLYALAKEKQ
                     GMLKQEKKEFGEQNAKKQLRIIQTTCILDQLVEFLACQGTDWIPVILPADATVPVDEW
                     TQKTWPENACMAVMTSGTSGKNKLLFRTFGSWYDYFPVQNEIFHINSERRLFMQGSLA
                     FTGNLNLYMGQLAAGATILAQERFDPRLWIRNIKQWQANGIYLIPAKLRALYQALCHE
                     KQAPLPEIRTILSGSQSLGKEEVAQLKQFFTEAEITLYYGASELSYVTYIRDTQMNDD
                     KTRIGRPFPGIDVQLEDGRLMVNTKWGVIGTGKEASAGDYAHLDEEGYFCFDGRKDDI
                     CSINGQKISCIRVEQAILSLGIGAEAAVKPVERQGRQYLAAWIVPKEKERGKDLEKKT
                     GEIRSQLKTILSQGEIPKFFFFEDTLPVNESGKVLKRLLE"
     gene            2662..>3343
                     /locus_tag="ACZPCL_12960"
     CDS             2662..>3343
                     /locus_tag="ACZPCL_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1163414.1"
                     /translation="MYQLVIVDDEDKIVEGIANLFPWEQLGFQVTWFSRPLKALEYVK
                     THQVHVLMSDIEMPEMNGLELCKQLQDSDIKIVFISSHQNYEYFRYAIQYRVEDYLLK
                     PIKSGDILNCFGKIRQQLDEKYAVTQETPVTYYDQVISKVKEYIKENYKEASLEDAAV
                     QVSLSPSYLSRIFKEKCGMGFSDYLTKTRMEKACELLGDIQYKSYDIAYYIGYDNPKN
                     FSRAFKAYF"
BASE COUNT         1035 a          611 c          858 g          839 t
ORIGIN      
        1 attagataga taagggagag cagacaatgg aaaatgtatg gatccttgga ggacttagaa
       61 gttttattgg tgtaaaagac gggatatacc ggcatattcc ggcggaagtt ctgggtgccc
      121 aggtcttaaa agagataaaa aacagatttt tgttaaaaga tgaagagatc gattatatca
      181 ttggcggaaa cgcagtagca ggcgggggaa atatcacccg ccttgctgcg cttacagccg
      241 gtttggatga aatgataccg gcagttactt tagatcttca gtgcggttcc gccctggaaa
      301 gtattactgc tgcagcagcg aagattgaaa gtggtctggc agatctggtg atcgcaggag
      361 gttttgaaag ttcctccacc cagccggtcc gcatgtggaa ttccaatcat ccggactatc
      421 aggaaggaaa aagctataca gtggcaaaat ttattcctga cattcaggga gagcaggtca
      481 tgctagaggg agcagaaaaa acagctctta cagaaaatgt caccaaagca gaaatggatt
      541 cctgggtatt gttaagccat aaaagagctg cccaggcggc aaaagaaggg attttatctg
      601 atatccagct ttccattgca gacagcaaaa aagatgaagg gatcagatct tccatgagcc
      661 agagactctt agacaggctt ccctgtttac tgccaggcgg ttcgattata acagctgcca
      721 atgcatgtct gatgcatgac ggggcagctt ttgtggtcct ctgttctgaa gcatacagaa
      781 aaaaacatgg tctgacgcct caggcagcgt tccgtttcgg cacagctatt ggcagtgatc
      841 cttttatgag tcctaaaaca gccgttctgg cgatccgcaa gctgttaaaa caaaccgctc
      901 tttctataga tgagatccac agtctggaag tgaatgaagc ctttgcagtg attgatgtac
      961 ttttagagcg ggagttccct ggaataagag agcggttaaa tccattggga ggagcccttg
     1021 cttacggtca tccttacggc gcttccggag ccattatcct gttacatctg ttaaaggatc
     1081 tggaactgaa ccagggaaga tttggcatct gcggagtagc ggcagcaggc gggatcggaa
     1141 cgaccctcct ggtggaaaaa gtataaagaa acgtatattg cggagataaa atccatggaa
     1201 aagatcacca actatctgga actgatacag cagatccagg atataacccc ggaaaaagag
     1261 gctttttgta caacaggcag aagtcttaca tacagccagc tttatgcttt ggcaaaagaa
     1321 aagcagggaa tgctaaaaca ggagaaaaag gaatttgggg aacagaacgc caaaaagcag
     1381 cttcggatca tccagacaac ctgtatttta gaccagctgg tggaatttct ggcctgtcag
     1441 ggaacagact ggatcccggt gatccttcct gcagatgcca cagtgccggt ggatgaatgg
     1501 acccaaaaaa catggcctga aaatgcctgt atggcagtta tgacttcagg gacatcagga
     1561 aaaaataagc tgcttttcag aacctttgga agctggtatg attattttcc tgtgcagaat
     1621 gagatttttc atataaattc agaaagacgc ctgtttatgc agggaagtct tgcatttaca
     1681 ggaaatttaa acctgtatat ggggcaatta gcggcaggag caactatttt ggcccaggag
     1741 cgttttgatc caaggctgtg gatccgaaat ataaaacagt ggcaggcaaa tggtatctac
     1801 ctgatcccgg caaaactgcg ggcattatat caggcattat gccatgaaaa acaggcacct
     1861 ctgccagaga tccggaccat cttaagcggt tctcagagcc ttggaaaaga agaggtagca
     1921 cagttaaaac agttttttac agaggcagag atcacgcttt attacggtgc cagtgagtta
     1981 agctatgtta cctatatccg tgatacacag atgaatgacg ataagaccag gatcggaagg
     2041 ccgtttccgg gaattgatgt gcagctggaa gacggaaggc ttatggtaaa tacgaaatgg
     2101 ggtgtgatcg gaacaggaaa agaagcaagt gcaggagatt atgcccattt ggatgaggaa
     2161 ggttattttt gttttgatgg cagaaaggat gatatttgca gcatcaatgg ccagaagatc
     2221 tcctgtatcc gtgtagaaca ggcgatcctt tcactgggga tcggggcaga ggcagcagta
     2281 aaaccggtag agcgacaggg acggcagtat ctggctgcct ggatcgtgcc aaaagagaaa
     2341 gaaaggggaa aagatctgga aaaaaagacg ggagagatcc gcagtcagtt aaaaacaatc
     2401 ctttcccagg gtgagatccc aaagtttttc ttttttgagg atacacttcc tgtaaatgaa
     2461 agtggaaaag tgttaaaacg gttattggag taatcattgt attttcagtt tttgtatggc
     2521 ataattaaat atcaggaaag ttttacaccg gtccctggtt gtcaggaccg gttccttttt
     2581 gctgtaaatg gatttacaag caagatgtat tcttaaaata aagcaatttg ggagtacagc
     2641 agggataaag ggaggaaaaa tatgtatcag ctggtgatcg tagatgatga ggataagatc
     2701 gtagaaggaa tagcaaatct gtttccatgg gaacagttag gatttcaggt aacgtggttc
     2761 agcaggcctt taaaggcatt ggaatacgta aagacccatc aggtccatgt gcttatgagt
     2821 gatattgaaa tgccggagat gaacgggtta gagctttgta aacagcttca ggacagtgac
     2881 atcaagatcg tatttatcag cagtcatcag aattatgagt atttccggta tgccatccag
     2941 taccgggtag aagattatct gttaaagcct atcaaaagcg gtgatatttt aaattgcttt
     3001 ggaaaaatac ggcagcagtt agatgagaaa tacgcagtta cccaggaaac accggtaact
     3061 tattacgacc aggtgatctc caaagtaaaa gagtacataa aagaaaatta taaagaggct
     3121 tctctggaag atgcggcagt ccaggtgtct ttaagcccca gttatctgtc ccgtattttc
     3181 aaggaaaaat gcggtatggg attttctgat tatcttacta agacaagaat ggaaaaagcc
     3241 tgtgagctgt taggggacat tcaatataaa agttatgata ttgcttatta tatcggctat
     3301 gataatccca agaacttttc cagggctttt aaggcctatt ttg
//
LOCUS       DBMDUA010000259         3334 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9955_length_3334_cov_2.076709, whole genome shotgun sequence.
ACCESSION   DBMDUA010000259 DBMDUA010000000
VERSION     DBMDUA010000259.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9955_length_3334_cov_2.076709"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="ACZPCL_12965"
     CDS             complement(<1..609)
                     /locus_tag="ACZPCL_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvfB family protein"
                     /protein_id="HIR1163415.1"
                     /translation="MIELGKTQELEIVRTKEFGVYLSEKAGDEAAVLLPKKQVPAGAK
                     IGDKVTVFIYKDSSDRLIATTGTPKLELGQCAVLEVKDVAKIGAFLDMGLEKDLLLPF
                     KEQTHPVRKGEKCLVCLYADKSHRLAATMKVYSHMSNQSPYKAEDWVEGTIYEINENI
                     GAFVAVDNKYYGLIPKKEMYGKYREGDTVKARVMRVREDGKLD"
     gene            complement(625..1341)
                     /locus_tag="ACZPCL_12970"
     CDS             complement(625..1341)
                     /locus_tag="ACZPCL_12970"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016297 - fatty acyl-[ACP] hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HIR1163416.1"
                     /translation="MYTFDSRIRYSETDETGALSLLGVINYMQDCSTFQSEDIGLGVE
                     YLEKKKRAWLLSSWRIVIDRYPVLGERIKIGTWATSSKGIYGYRDFVLMDQEGNYLVK
                     AESVWFFCDTEKMIPVRVMPEDVEAYGEGKALDLGKAPRKIAVPENYEEETPVTIAVH
                     HLDTNHHVNNAQYVDIAREAVTCTKKVKEIRVDYKKAAVLGEVLIPRVTKTGEDERTI
                     VLAGEDKEIRAVVWLKYEEA"
     gene            complement(1494..1566)
                     /locus_tag="ACZPCL_12975"
     tRNA            complement(1494..1566)
                     /locus_tag="ACZPCL_12975"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1532..1534),aa:Gln,seq:ctg)
     gene            complement(1605..1677)
                     /locus_tag="ACZPCL_12980"
     tRNA            complement(1605..1677)
                     /locus_tag="ACZPCL_12980"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1643..1645),aa:Gln,seq:ctg)
     gene            complement(1715..1787)
                     /locus_tag="ACZPCL_12985"
     tRNA            complement(1715..1787)
                     /locus_tag="ACZPCL_12985"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1753..1755),aa:Gln,seq:ctg)
     gene            complement(1916..>2120)
                     /gene="aspA"
                     /locus_tag="ACZPCL_12990"
     CDS             complement(1916..>2120)
                     /gene="aspA"
                     /locus_tag="ACZPCL_12990"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585256.1"
                     /note="catalyzes the formation of fumarate from aspartate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HIR1163417.1"
                     /translation="VITAICPYVGYETAANIAKEALRTGRQVRDLILEKGLMDEKQLN
                     TVLNPYTMTEPGILGKDPMDQLR"
     assembly_gap    2121..2130
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2131..>3334)
                     /locus_tag="ACZPCL_12995"
     CDS             complement(<2131..>3334)
                     /locus_tag="ACZPCL_12995"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006531 - aspartate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HIR1163418.1"
                     /translation="TEEKKYRIEHDSIGEKEVPIDAYYGVQTLRAHENFYITGLKMHP
                     ELIKSVAQIKKAAAITNFEVGELDKKRASAITQACDEIIAGKLHDQFIVDPIQGGAGT
                     SLNMNANEVIANRAIEILGGKKGDYSLVNPNDHVNFGQSTNDVFPSCGKMAALKLISN
                     AQEQLKRLEKALMDKSKEFDAVIKMGRTQLQDAVPIRLGQEFHAYASAIRRDIKRFDN
                     AKEEIRCLNLGGTAIGTGLNADVQYFHRVVKNMSTLTGEELVQSYDMIDATQNLDSYA
                     SVSGIVKNCAVNLSKMCNDLRLMSSGPRTGFGEINLPARQNGSSIMPGKINPVIPEVV
                     NQVAFNIIGNDMTITMAAEAGQLELNAFEPIIFYNLFESLETLASAVNTLVDNCIVGI
                     TANEEHCRK"
BASE COUNT          801 a          829 c          633 g         1061 t
ORIGIN      
        1 gtctaacttt ccgtcttcgc ggactctcat gactcttgcc ttaacagtat ctccttcgcg
       61 gtactttcca tacatctctt ttttagggat caaaccataa tatttattat caactgcaac
      121 gaaagcacca atattttcat taatctcata aatagtacct tctacccagt cttctgcctt
      181 gtacggagac tgattactca tatgagagta gaccttcatg gttgcagcca gtctgtggct
      241 cttgtctgca tagagacata ccagacattt ttctcccttg cgtaccggat gggtctgctc
      301 tttaaatgga aggagcaggt ctttttccag acccatgtca aggaatgcac cgatctttgc
      361 cacatccttt acttccagga ctgcacactg gcctaattcc agctttggag tacctgtggt
      421 tgcgatcaga cggtcggaag agtccttgta aataaataca gtcaccttat ctccgatctt
      481 tgctcctgca ggaacctgct ttttaggaag aaggactgct gcttcatctc ctgctttctc
      541 acttaagtac acgccaaatt ctttggtgcg cacgatctct aattcctgtg tctttcctaa
      601 ttctatcata tatttatact ccttttatgc ttcttcgtac ttaagccaga ccaccgcacg
      661 gatctcttta tcttctccgg ccaacacaat ggtcctctca tcttcccctg ttttcgtcac
      721 tcttggtata aggacttccc ctaaaacagc tgccttttta taatccaccc ggatctcttt
      781 tacctttttc gtacaggtta ctgcttccct tgcaatatct acgtactggg cattatttac
      841 atggtggttg gtatctaaat gatggactgc aatagtaacc ggtgtttcct cttcataatt
      901 ttccggaaca gcgatctttc ttggtgcctt tcccagatcc agcgctttcc cttctccgta
      961 ggcttctaca tcttccggca ttacacgcac cgggatcatc ttttccgtgt cacagaaaaa
     1021 ccagacggac tctgccttta ccaggtaatt tccttcctgg tccataagta caaaatcacg
     1081 gtaaccgtaa attcctttag aagaagtagc ccaggttcct atttttatcc tttctcccag
     1141 gaccggatat ctgtcaataa cgatcctcca ggaagacaac agccacgccc tcttcttttt
     1201 ttccagatat tctactccca ggccaatatc ctctgactga aacgtgctgc agtcctgcat
     1261 ataattgata actcccagca aacttaacgc accggtctca tctgtctcac tgtaacggat
     1321 cctgctgtca aatgtataca tgcatttcct ctgtttcttt ttagtgtttc ttcttattat
     1381 aaatgtgtta tcatagataa ccgcttttct ccccaagtat aacacggggc ggttacaggt
     1441 gcaacaaaaa agtctttcat ttccaaagct cagaaatcaa agactctctc aaactgggct
     1501 agaaggattc gaaccttcac aatgacggag tcagagtccg ttgccttacc atttggcgat
     1561 agcccattat aatatgaagt ttttaaaagc gctttgcgct ttagctgggc tagaaggatt
     1621 cgaaccttca caatgacgga gtcagagtcc gttgccttac catttggcga tagcccatta
     1681 taatatgaaa ttttaaaagc gttttgcgct ttagctgggc tagaaggatt cgaaccttca
     1741 caatgacgga gtcagagtcc gttgccttac catttggcga tagcccatta ttttgtcttc
     1801 gcagtctcaa tcgcgactgc gaagattatt atacatataa taccagttct agtcaagcac
     1861 ttttttaatt tttttgtatt tttttgtatt tttgtcccaa ttttattctg atcatctaac
     1921 gaagctggtc catagggtcc tttccaagga tacccggctc tgtcatggtg tatggattta
     1981 atactgtatt taactgtttt tcatccatca gtcccttttc caggatcaga tcacggacct
     2041 gtctgccggt gcgcagcgct tcttttgcaa tattggcagc tgtctcatac cctacataag
     2101 gacagatcgc agtaataaca nnnnnnnnnn gcttacggca gtgttcttca tttgcggtaa
     2161 ttcctacaat acagttatct accagtgtat taacggctga tgccagtgtt tcaagagact
     2221 caaacaggtt atagaaaatg atcggctcaa aggcatttaa ttccagctgt cctgcttccg
     2281 ctgccatggt aatagtcata tcatttccta taatattaaa tgcaacctgg tttacaacct
     2341 ccgggatgac cgggttgatc tttcctggca taatggaaga gccgttctgt cttgcaggca
     2401 aattgatctc tccaaagccg gtccttgggc cggatgacat aagacgcagg tcattacaca
     2461 tcttggaaag gttcactgca cagtttttca cgataccgga tacagaagca tagctgtcca
     2521 ggttctgggt tgcatcaatc atgtcatagg actgcaccag ttcttctcct gtcaatgtgg
     2581 acatgttttt aactactctg tggaaatact gtacatctgc gttcaagccg gttccaatag
     2641 cagttccgcc taagttcaag cagcggatct cttcttttgc attgtcaaaa cgcttgatat
     2701 cgcggcggat cgcagatgca taggcatgaa attcctgtcc taaacggatg ggcactgcat
     2761 cctgaagctg ggtccttccc attttaataa cagcgtcaaa ttcctttgac ttatccatta
     2821 atgccttttc cagacgtttt aactgctcct gggcattaga gatcagcttt aaggctgcca
     2881 tcttgccaca ggacgggaat acatcgttgg tagactggcc gaagtttaca tggtcatttg
     2941 gatttaccag gctgtaatcg ccctttttgc cccctaagat ctcaatggca cggtttgcga
     3001 tcacctcatt ggcgttcata ttcagggaag ttcctgcacc gccctggatc gggtcaacaa
     3061 taaactggtc gtgaagcttc cctgctatga tctcatcgca ggcctgtgta atagcagaag
     3121 cacgcttttt atccagctct cctacctcaa aattggtaat agctgctgcc tttttgatct
     3181 gggctacact tttgatcagc tccggatgca tttttaagcc tgtaatatag aaattttcat
     3241 gggcacgcag agtctgtacc ccataataag catcaatagg tacttccttt tctccaatag
     3301 agtcgtgctc tatgcggtat ttcttctctt ctgt
//
LOCUS       DBMDUA010000260         3323 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_9991_length_3323_cov_2.842024, whole genome shotgun sequence.
ACCESSION   DBMDUA010000260 DBMDUA010000000
VERSION     DBMDUA010000260.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9991_length_3323_cov_2.842024"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="ACZPCL_13000"
     CDS             complement(<1..507)
                     /locus_tag="ACZPCL_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HIR1163419.1"
                     /translation="MFQGLFNYDNPVWRFIGKLGDLVLLNLLWVICCIPVFTAGAATT
                     AVYYVTLKLVRDDDGSTIKSFFHSFTQNFKQATAIWLMILVAGGLLGFDLWFLMSGVV
                     TVSTVVKTVMSAVFGAMTLILLFVTTYIFPLQARFYNTVKRTLFNAFFMSVRHLLQTI
                     SMLIIDGLV"
     gene            806..1489
                     /locus_tag="ACZPCL_13005"
     CDS             806..1489
                     /locus_tag="ACZPCL_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163420.1"
                     /translation="MELKKDIKSELEKIKQMSGKDRIWYIYEYYKFHIVAVILAFTLL
                     WVVGSSLYRQTFTTRLTFAIVNDRSGGESSMDEFENSLKEALGFGKKDLIEINSGLFI
                     NADGSQSSEYSYASMAKIAALAAGGDLDIIIADAKTLEHYESQGAFLDLKEFLPQDLL
                     ERAEKEDLFFYTDNEKGQSTAAAISLEPTAFSDMTGVSIQPPYLAVIASSQHTEDTLR
                     AIQWLFDQK"
     gene            complement(1422..2192)
                     /locus_tag="ACZPCL_13010"
     CDS             complement(1422..2192)
                     /locus_tag="ACZPCL_13010"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1163421.1"
                     /translation="MKQERTLSIEGKEYTLLISDEPQALLEAKASGRAVLGCLDTGNG
                     EGASRDFQGIPYVVPSMEYVTEELLELILRRYLGLPWLIDETERLYIREFVKEDAENI
                     PEEEYGKEEEIFRDPDKLEVYIKNQYGFYEYGTWAVLKKSPSLLIGMAGVSNPNLPET
                     ALQALPTLPDDLCWLELGYHIFKPYRKNGYATEAAKAILSYSHEVLSARLCAWIEAKN
                     KASCALAESLGMEVVKVISDQTATGWPGAYLLYAEKMQ"
     gene            complement(2265..2783)
                     /locus_tag="ACZPCL_13015"
     CDS             complement(2265..2783)
                     /locus_tag="ACZPCL_13015"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1163422.1"
                     /translation="MANPVVTIIMENGDVMKAELYPEVAPNTVNNFISLVKKGYYDGL
                     IFHRVIRGFMIQGGCPQGTGMGGPGYSIKGEFSQNGFKNDLKHTEGVLSMARSMMPNS
                     AGSQFFIMHKDAPHLDGAYAAFGKVTEGMDVVNKIAEERTDYSDRPLKTQKIKSMTVD
                     TFGVEYPEPEKY"
     gene            complement(2897..3211)
                     /locus_tag="ACZPCL_13020"
     CDS             complement(2897..3211)
                     /locus_tag="ACZPCL_13020"
                     /inference="COORDINATES: protein motif:HMM:NF014177.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HIR1163423.1"
                     /translation="MMMKSCKLKILKNSCFKNICFLDPPYDTDFSTYAQNEFGHEEQK
                     RLCECLKRTPAKILLIIKNTDFIYELYKDNFNITSFDKKYMVSFMNRNDRKAEHLIIT
                     NY"
BASE COUNT          935 a          747 c          633 g         1008 t
ORIGIN      
        1 tacaagacca tcaatgatca gcatggaaat ggtctggagc agatgacgca cagacatgaa
       61 aaatgcatta aataatgtac gtttcactgt gttataaaat cttgcctgta atggaaaaat
      121 ataagtagta acaaacagca agatcaatgt catagcacca aatacagctg acatcactgt
      181 ttttacaacc gttgatacag ttaccactcc tgacataaga aaccacagat caaatcccag
      241 caatccgcca gctacaagga tcatcagcca gatcgctgtt gcctgcttaa agttctgggt
      301 aaaggaatgg aaaaatgact tgatggtaga accatcatcg tcacgtacca gttttaaggt
      361 aacataatat accgctgtag tagctgctcc tgctgtaaat acagggatgc agcagatcac
      421 ccacaaaaga tttaaaagta ccagatctcc cagttttcct ataaaacgcc acaccgggtt
      481 atcataatta aaaagaccct gaaacatatt ttatcctctt ctttcctgct tacgcagccg
      541 cttattttcc actgcgctca tttctatata ctcaggtcca cgcactgacg tgctctactg
      601 cctgcttcgc aggcggaacc ttcgttaatg gcttgtgtaa aataaatgtc tgcttacgca
      661 gccgcttatt ttccactgcg ctcattacat tgtatcatat taagtgtaaa aatggaacga
      721 gtagttgcgg gtatctgcat tttcagctat actgtagtgt aatgagcgca gaaaaatcca
      781 gacgtcaacc tgaaaggaat acatcatgga actgaaaaaa gacataaaaa gtgaacttga
      841 gaaaataaaa caaatgtccg ggaaagaccg gatctggtat atttatgaat attataaatt
      901 tcatattgta gctgtcattc tggcatttac ccttttatgg gtagtaggaa gcagcctgta
      961 tcgccagacc tttaccacac ggcttacttt cgccattgtc aatgaccgtt ccggcggtga
     1021 gtcatctatg gatgaatttg aaaacagctt aaaagaagct cttggttttg gtaagaagga
     1081 tctgatcgag atcaattccg gactgtttat caatgctgat ggctcccagt cttccgaata
     1141 ttcctatgca agtatggcaa agattgctgc tttagcagcc ggaggagacc tggatattat
     1201 catcgctgat gcaaagacac tggaacatta tgagtcccag ggcgcatttt tagatctgaa
     1261 ggaattcctg ccccaggatc tgttagaacg tgccgaaaaa gaagaccttt tcttctatac
     1321 agacaatgaa aaagggcagt caactgctgc cgctatttca ctggaaccta ctgcattttc
     1381 cgatatgacc ggtgtctcga tccagccgcc ctatctggca gttattgcat cttctcagca
     1441 tacagaagat acgctccggg ccatccagtg gctgtttgat cagaaataac ttttactact
     1501 tccattccaa ggctttctgc cagtgcgcag gaggccttgt tttttgcttc aatccaggcg
     1561 cagagtctgg cagaaaggac ttcgtgggaa taagaaagaa tggctttagc tgcttctgtt
     1621 gcatagccat ttttcctgta aggtttaaaa atatggtatc ccagttccag ccagcaaagg
     1681 tcgtctggaa gagtgggtag cgcctgcaaa gcagtttccg gaagattggg gttactgacg
     1741 ccggccatgc cgatgagaag cgacggactt ttctttaaca cagcccaggt cccatattca
     1801 taaaaaccat actggttctt tatatatact tctaatttgt ccggatcacg gaatatctct
     1861 tcttccttac catattcttc ttctggtatg ttctctgcat cctcttttac aaattcacgg
     1921 atgtacagtc gctctgtctc gtcaataagc cacggaagtc ccagataccg gcgcaggatc
     1981 agttctaaaa gttcttctgt cacatattcc attgagggaa ctacataagg gatcccctga
     2041 aaatctctgc tcgctccctc cccatttccc gtatccagac aacctaaaac agcccttccg
     2101 gaagcttttg cctccaaaag ggcctgtggt tcgtcagata tgagaagcgt atattctttt
     2161 ccttctattg aaagagtccg ctcctgcttc atattgttct gtctcctgta agaaccgttt
     2221 tttactattt actgctgttt tgccgttata tcaccggcaa tctatcagta tttttctggc
     2281 tctgggtatt ctacaccaaa agtatctact gtcatagatt tgatcttctg tgtctttaat
     2341 ggacggtcag aataatcggt gcgctcttct gcgatcttat ttacaacatc cattccctct
     2401 gtaaccttgc caaatgctgc ataagcgccg tccagatgag gagcatcttt gtgcatgata
     2461 aagaactggg agcctgcaga gttaggcatc atggatcttg ccatggataa aactccctcg
     2521 gtgtgcttta aatcattctt aaaaccattc tggctgaact cacccttaat actgtaacct
     2581 gggccaccca taccagttcc ctgagggcaa ccgccctgga tcataaatcc acggatgact
     2641 ctgtggaaga tcaggccatc ataataacct ttctttacca ggctgataaa gttatttact
     2701 gtgtttggtg ctacttccgg atataattct gctttcatca catcaccatt ttccatgatg
     2761 atagtaacaa ctggatttgc cattgttttt cttctccttt atgcttcaga attggcctgt
     2821 ggggtgcagg ctctttttgt ttaatatagc agattgtagg cgggttgtcc agtgaagtgg
     2881 aaaccactta tacgctctaa taattcgtta taattaaatg ttctgcctta cgatcatttc
     2941 tattcataaa actaaccata tattttttat caaaacttgt tatattgaaa ttatctttat
     3001 ataattcata gataaaatcc gtatttttaa taatcagtaa tattttggcg ggcgtccgct
     3061 ttaaacattc acacagacgt ttttgctcct catgtccaaa ctcattctgt gcataagtag
     3121 aaaagtctgt atcataaggc ggatccaaga aacaaatatt tttaaagcag ctatttttga
     3181 gtatctttag tttacacgac ttcatcatca tcgtcaatca tttcactttc gtcacttaga
     3241 atcaatttga ctaaattaaa ttgttattca aaagccaaaa aggatgtcac tccttgcgaa
     3301 gcaacatcca gtcccattta ttt
//
LOCUS       DBMDUA010000261         3311 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_10033_length_3311_cov_2.487539, whole genome shotgun sequence.
ACCESSION   DBMDUA010000261 DBMDUA010000000
VERSION     DBMDUA010000261.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10033_length_3311_cov_2.487539"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="ACZPCL_13025"
     CDS             complement(<1..473)
                     /locus_tag="ACZPCL_13025"
                     /inference="COORDINATES: protein motif:HMM:NF014518.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HIR1163424.1"
                     /translation="MSSVSSTSSSLGNTALRGYGGLASGIDRDALIEQMTARTTSKIT
                     AKKKAMTKLEWKRDAYRSVSNKIIDLQDNYLSYSATKSLKNSDFFAKNQVSVQGDPDY
                     TKYISATGNADTASRVSVLGVNKLATSATLISGEKKTDSAITLGGISESDFSNKE"
     gene            complement(592..1284)
                     /locus_tag="ACZPCL_13030"
     CDS             complement(592..1284)
                     /locus_tag="ACZPCL_13030"
                     /inference="COORDINATES: protein motif:HMM:NF022179.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HIR1163425.1"
                     /translation="MAKKTNKTSHVLSLITGSQPPVAPSGETQQEGSAAEEPVRVPSA
                     NVTVVDPREEGKLAEEIQKNLIRELEKEQTEEQAEQPREQEEQSEEQTERSEEQTEQP
                     NEQAEQTQEQTEQPQTNAGIPVQKEPEQQEEQKQEDRPSYRVVNIMEEILTPELILDA
                     LKNNDTCTCSRCQADVKALMLTRLPPKYIVADNTAVPMLLTYYHNKFRVAVLSQSIRA
                     CMDVKERPRHIL"
     gene            complement(1286..2056)
                     /locus_tag="ACZPCL_13035"
     CDS             complement(1286..2056)
                     /locus_tag="ACZPCL_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR1163426.1"
                     /translation="MVITVSNQKGGVGKTTTSAALAAGFSMAGKKVLCIDLDPQGNLG
                     FCLGLDTEGGSTILDVLKRDVSIQKAISRTDGIDILPSDITLSSSGLEYKQGEEKESI
                     LKDVLKPVIDFYDYIVIDTPPALNLLTVNAYAVSDYLIIPMASDILSLVGLSQLKETV
                     ESVRIQINPGLKIMGILLNRFNPRTCLCRDVLEMAGQLAGQLETRVFDTKIRSGVAIA
                     EAPAHGESIFEYSPRSSAVKDYVKFLDEVEKIIGFREK"
     gene            complement(2117..3256)
                     /locus_tag="ACZPCL_13040"
     CDS             complement(2117..3256)
                     /locus_tag="ACZPCL_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HIR1163427.1"
                     /translation="MEKYVLTQDLLTGNELIDSEHRKIFDEVNTLLDACSKGKGREKI
                     SSLGEFLVEYVTKHFSDEEDLQKQSKYPEYTEHHKFHEWYKQKLGDAIIKLEQEGPTI
                     NSLGEINYMVSVLVKHIRETDRKLAQWIQNGAKNKETASKAATGSAVSGKTSYIDIVS
                     KDESTENLDIRQILDIKELQRIQDLFLAATGMTAAVVDMKGKYITRGSSFTSFYSRYT
                     NGKHNELREFTQDLTVGGFRAGTVIGGIVLGDYPDDSAPGQEAVSAGGQLLAEMLNLW
                     ANALYYRKSNADSVEAFAKEEKKVRDAVGQIKYKAKGLEQTATMEKMLSLNAAIEAGR
                     AGKAGVGFGVVADEIGRMANESAAVYQEIQELVKQVEESMERLGK"
BASE COUNT          734 a          819 c          630 g         1128 t
ORIGIN      
        1 atttctttat tgctaaaatc agattcactg atgccgccta aggtaatagc tgaatctgtt
       61 ttcttttcgc cggagatcaa tgtggcagat gttgctaatt tgtttactcc cagaactgat
      121 actctggatg cagtgtctgc atttcctgtc gctgatatat attttgtata atcaggatcc
      181 ccctgtacac tgacctggtt ttttgcaaag aaatcactgt tttttaaact ctttgttgct
      241 gaatagctaa ggtaattgtc ctgaagatct atgatcttat tgctgacaga acgatatgca
      301 tcccttttcc attccagttt ggtcatagcc ttcttcttgg ctgtgatctt ggatgttgtt
      361 cttgctgtca tctgttcgat cagtgcatcc ctgtcaatac cggaagctaa accaccatat
      421 cctcttaaag ctgtatttcc aagactgctg cttgtacttg atacacttga catatctgcc
      481 gctcctttca gtcatgtgat gtcgcggtgt acataaaatg cgccgcttct atatatttta
      541 tccctgtgat cctatagatt ctatatacat atttcggcgc agcatgaaaa tttataagat
      601 atggcgtgga cgttctttta catccataca ggctcttatg gactgactta aaacagccac
      661 acggaattta ttgtggtaat aggttaacag catcgggact gccgtattat ctgctacaat
      721 atatttaggt ggcagcctgg taagcatcag tgccttcaca tctgcctgac agcggctaca
      781 ggtacaggta tcattatttt tcagtgcgtc aaggatcagt tccggagtaa ggatctcctc
      841 catgatattt acgacccggt aacttggacg atcttcctgc ttctgctctt cctgttgttc
      901 tggttctttc tgtacaggta tccctgcgtt tgtttggggc tgctctgtct gctcttgtgt
      961 ctgttctgcc tgctcatttg gctgctctgt ctgttcttct gatcgttctg tctgttcttc
     1021 tgactgttct tcctgctccc tgggctgttc tgcctgctct tctgtctgtt ctttttccag
     1081 ctcccggatc agattcttct ggatctcttc tgccagcttt ccttcttctc ttggatcaac
     1141 tacagttaca ttagcggaag gtactcttac cggttcttca gcagctgagc cttcctgctg
     1201 tgtttcgcca gaaggcgcaa ctggcggctg tgaacctgtg atcaggctca gaacatgtga
     1261 ggttttattt gttttttttg ccatattatt tctccctgaa acctatgatc ttttctacct
     1321 catctaaaaa ctttacataa tcctttacag ctgatgatct tggactgtac tcaaaaatac
     1381 tttctccatg ggccggtgct tctgcaatag ccactccgga ccttattttt gtgtcaaata
     1441 cccgggtttc cagctgcccg gccagctgcc cggccatttc caatacatcc ctgcaaagac
     1501 atgttcttgg attaaagcgg tttagcagaa tacccatgat cttaaggccg ggattgatct
     1561 gtatgcggac agattctacg gtttccttta actgtgacag accaaccaga ctgaggatat
     1621 ccgatgccat tggtataatg agataatcag aaactgcata tgcattaact gtaagaagat
     1681 ttaatgcagg cggagtatcg atcacaatat aatcataaaa atcaattacc ggctttaaaa
     1741 catctttcaa aatagattct ttttcttctc cctgcttata ttccaggccg ctgctgctta
     1801 aagtaatatc ggaaggaagg atatcgatcc cgtctgtgcg gctgatagcc ttttgtatgg
     1861 atacatctct ttttaataca tccaatatag tagatcctcc ttcggtatcc agaccaaggc
     1921 agaatccaag atttccctgt ggatccaggt ctatgcacag aactttttta cctgccatcg
     1981 aaaatcctgc cgccagcgct gctgatgttg tggtctttcc cacaccgcct ttttgatttg
     2041 aaaccgtaat gaccatttat gtatctcctt ttatcctctc ttctgcatac aatattttct
     2101 gaaagatctc cctgtattat ttaccaaggc gctccataga ttcctctacc tgttttacca
     2161 gttcctggat ctcctgatac acagcggctg actcatttgc catacgtccg atctcatctg
     2221 ctacaacacc aaagcctaca cctgcttttc cggcacggcc tgcttcaatg gctgcattca
     2281 gggaaagcat tttttccatc gtagccgtct gctccaatcc ctttgcctta tacttgatct
     2341 gtcctactgc atcccgtact ttcttttcct cttttgcaaa tgcttctacg ctgtctgcat
     2401 tgctcttccg gtaataaagg gcattggccc acagattcag catctcagct aaaagctgtc
     2461 caccggcgct taccgcttcc tggcccgggg ctgaatcatc cgggtaatct ccaaggacga
     2521 taccgccgat cactgtacct gcacggaatc ctcctactgt aagatcctgg gtaaattcac
     2581 gcagttcatt gtgttttcca ttggtataac ggctgtaaaa gctggtaaag gagcttcccc
     2641 ttgtaatgta ttttcctttc atgtccacta ctgctgctgt catgcctgta gctgctaaaa
     2701 acagatcctg gatccgctga agttctttta tatccaggat ctggcggata tccagatttt
     2761 ctgtactttc atcttttgaa acaatatcta tataagaagt tttccctgat actgctgatc
     2821 ctgttgctgc tttagaagct gtctctttat ttttggcccc attctggatc cactgggcca
     2881 gcttgcggtc tgtttcccgg atatgcttaa ccagcacgct taccatatag ttgatctcgc
     2941 ccagagaatt gatcgtcggg ccttcctgct ccagctttat aatggcatca cccagtttct
     3001 gtttatacca ttcatgaaat ttatgatgtt cggtatattc cggatatttt gactgcttct
     3061 ggagatcctc ctcatctgaa aagtgtttcg ttacatattc aacaagaaat tctcccagac
     3121 ttgagatctt ttctcttccc tttccttttg agcatgcatc cagaagggta ttgacttcgt
     3181 caaaaatctt cctgtgctca ctgtcgatca gttcatttcc tgttaaaaga tcctgtgtta
     3241 atacatattt ttccatcctt agagtcctcc ttactgctga ctgtgctgtt ctttgaaacg
     3301 ggcaagcagt t
//
LOCUS       DBMDUA010000262         3307 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_10044_length_3307_cov_3.269183, whole genome shotgun sequence.
ACCESSION   DBMDUA010000262 DBMDUA010000000
VERSION     DBMDUA010000262.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10044_length_3307_cov_3.269183"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            99..242
                     /locus_tag="ACZPCL_13045"
     CDS             99..242
                     /locus_tag="ACZPCL_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163428.1"
                     /translation="MYSRLVIDGNAVYEIDEECVQKKKNRGYKAGKRRGEERKEEKGV
                     FPK"
     gene            complement(292..525)
                     /locus_tag="ACZPCL_13050"
     CDS             complement(292..525)
                     /locus_tag="ACZPCL_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004462337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163429.1"
                     /translation="MSDVAATNCGCDCGCGCQSGGNGCNIIWLILILCCCGGGNGCGG
                     CFGNNGCGNGCGCDIIWILLLLCCCGGGNGGFC"
     gene            729..1907
                     /locus_tag="ACZPCL_13055"
     CDS             729..1907
                     /locus_tag="ACZPCL_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR1163430.1"
                     /translation="MDQNRLYYATPYVKTFMCTVISCTKNKKGTWDTILNQTAFYPEG
                     GGQPSDTGALNGVKVLHVSEKGEEIIHELEAPLEEGVLAEGVIDWQKRYDNMQQHTGE
                     HIFSGLVHKHFGYDNVGFHMGTDEVTVDFNGVLTQEQLDELEDEANQLIYDNVPVKVF
                     YPSKEELEELDYRSKKELTGLVRIVEIPGGDICACCGTHVETTGEVGLIKLRTMINYK
                     GGVRISMLCGRRALMDYRERLKDEIRISNLLSAKLALVPDAVEKMKNESQEKDLALGR
                     LWQQLLEKKAESYPESTEVLAVFEEGLSPVQLRQLATMLYEKGKGKIVGVFSGNEDEQ
                     VYQYALGSSQADMRKLSKAMNGALNGRGGGSNLMAQGTFKAAESAIRETLMQEAGNLE
                     "
     gene            complement(1996..2232)
                     /locus_tag="ACZPCL_13060"
     CDS             complement(1996..2232)
                     /locus_tag="ACZPCL_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polya polymerase"
                     /protein_id="HIR1163431.1"
                     /translation="MKIQNITDVEKFFKVIDQCKGTVELVSPEGDRINLKSKLAQYLS
                     MATIFSNGYIKELDLVAHDKEDIERLIKYMYQGE"
     gene            complement(2355..3212)
                     /locus_tag="ACZPCL_13065"
     CDS             complement(2355..3212)
                     /locus_tag="ACZPCL_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR1163432.1"
                     /translation="MGFVDLHVHSNASDGTFSPSQVVELAKNAGLDAFALTDHDTTAG
                     VPEALEKGRDLNIEVIPGIEVSSSFDGTEIHILGLFVNSDDPVLAAMLEKMRISRDRR
                     NEKMLENLAADGISFTKEEICGDNPDTIITRAHIAHALVAKGICSGMDQAFKKYLQYG
                     GRYCPQKEHLSPEEVVKTLISNGAFVALAHPFQYKFGDKKTEELIAHMADLGMKGLEV
                     YHSSNNKLESMKLQEMAVRHHLLPTGGSDFHGGNKPDISIGTGRGGLRVSSLLLEDIK
                     RERAKQALS"
BASE COUNT          971 a          645 c          797 g          894 t
ORIGIN      
        1 taaacttatg cggcaacctt ttatttcatg catatattgt agtatgagga tgtactctta
       61 aaaggtttgg gagtccattt tctgaaagga ggttcattat gtatagcaga ctggtgatcg
      121 acgggaatgc tgtatatgaa atagatgagg aatgtgtaca aaaaaagaaa aacagaggtt
      181 ataaggcagg aaagagaaga ggagaagagc gaaaagaaga aaaaggagtt tttccaaaat
      241 gaaaacaggg gcgcataaac gccccgttgt tttctgactt tctgaggaag atcagcagaa
      301 gccgccattg ccgccgccac agcagcataa aagcagcagg atccagataa tgtcgcatcc
      361 acatccgttt ccgcagccgt tatttccaaa acagccgcca catccatttc caccgccaca
      421 acagcataag atcaggatga gccagataat attgcatcca tttcctccgc tctggcatcc
      481 gcacccgcag tcacagccgc agttagttgc tgctacatca ctcattttag ttcctccgaa
      541 atatgtttga ttacaatgct atcatatgtc aggcagcttg cttatgtttc ttgtttttcg
      601 ggaaaaatta aatttaaaga gaattttaaa taaagataag ggataaaggt ggtataatga
      661 tagggcgaaa aagaaagaca agagccgcag acgcggcgtt ttataatcaa aggatggagg
      721 aatgacctat ggatcagaac cgtttatatt atgcaacccc ttatgtaaaa acatttatgt
      781 gtaccgtaat ttcatgtaca aaaaataaaa aaggaacctg ggatacgatc ttaaatcaga
      841 ctgcttttta tccggaaggc ggcggacagc caagtgatac aggtgcttta aatggtgtaa
      901 aagttttaca tgtcagcgaa aagggagagg agatcatcca cgaactggag gctcctttgg
      961 aagaaggggt tctggcagag ggagtcattg actggcagaa acgttatgac aatatgcagc
     1021 agcacacagg agaacatatt ttttccggtc tggtacataa acattttggc tatgacaacg
     1081 taggtttcca tatgggaaca gatgaagtga cggtggattt taacggtgtt ctcacccagg
     1141 agcagttaga tgagctggaa gatgaagcga accagctgat ctatgataat gtgcctgtaa
     1201 aggtgtttta tccttcgaaa gaagaactgg aagagctgga ttaccggagc aaaaaagaac
     1261 tgaccggtct ggtacgcatt gtagagatac caggcggaga tatctgcgcc tgctgtggca
     1321 cccatgtgga gacaaccggg gaagtaggtc tgatcaaact gcgtactatg atcaactata
     1381 aaggaggcgt gcgtatttcc atgctctgtg gacgccgggc actgatggat tacagggaac
     1441 gtttaaagga tgagatccgt atttccaatc tgctttctgc aaaactggct ctggtaccgg
     1501 atgcagtgga aaagatgaag aatgaaagtc aggaaaaaga tctggcttta ggacgcctgt
     1561 ggcagcagct tttggaaaaa aaggcggaaa gctatccgga aagcacggag gtacttgctg
     1621 tatttgaaga gggattgtca cctgtacagc ttcgccagct ggctaccatg ctttatgaaa
     1681 agggaaaagg aaagatcgta ggagttttct ccggaaatga ggatgaacag gtatatcagt
     1741 atgctttagg cagcagtcag gcagatatgc gcaaattaag caaggcaatg aacggtgctc
     1801 ttaatggaag aggcggcgga agcaacctga tggcgcaggg aacttttaaa gcggcagaaa
     1861 gtgcgatcag agaaacactg atgcaggaag ctggaaatct ggaataatag taggaataat
     1921 aataaaacag ctgcgggaat ccagatagat gggagcctgc agctgttttt gtgccaaaac
     1981 acatttgttt atgatttatt caccctgata catatatttg atcagacgtt caatgtcttc
     2041 tttatcatgt gctaccaggt ccagttcctt gatatagcca ttggaaaaaa tagtagccat
     2101 ggacaggtac tgagccagtt tagacttcag gttaatgcgg tcgccttctg gagatactaa
     2161 ttctacggtt cccttgcact ggtcgatcac tttgaaaaat ttctctacat cagtaatatt
     2221 ctggatcttc atggtaattc ctccttctga attacatcta ttatcatttg tttagttaat
     2281 cttttaacga ttgcattata acaaagatga gactctgata caagggtaaa attttgtgat
     2341 ttttagcgaa atttttatga aagcgcctgt tttgctcttt cacgtttgat atcttccagt
     2401 aaaagggaag aaactctgag tccgccccgt cctgtaccaa tcgaaatatc cggtttgttc
     2461 ccgccgtgga agtcggaacc gccggttggg agcaggtgat gtctgacagc catttcctga
     2521 agtttcatgc tttctaactt gttgttggag gagtggtaaa cttccagtcc cttcataccg
     2581 aggtcggcca tgtgggcgat cagttcttcc gttttcttat ctccgaattt gtactggaaa
     2641 ggatgggcta aggccacgaa agcaccattg gagatcagcg tttttacaac ttcttccgga
     2701 gagagatgct ctttctgcgg acaatagcgg ccgccgtact gaagatattt tttaaatgcc
     2761 tggtccattc cggaacagat gccttttgcc actaaagcgt gggcaatatg tgcccgtgtg
     2821 ataatggtat caggattatc tccgcaaatt tcttcctttg taaaggagat cccgtcagcg
     2881 gccagatttt ccagcatctt ttcattgcgg cggtcacggc tgatacgcat tttttccagc
     2941 atggcggcaa ggaccggatc gtcagaattt acgaaaagtc ctaaaatgtg gatttctgtt
     3001 ccatcgaaac tactggacac ctcaattccg gggatcactt ctatattaag atcacgtcct
     3061 ttttctaatg cctcaggtac accggcagtg gtgtcgtggt ctgtaagggc aaaggcatcc
     3121 aatccggcat tttttgccag ttccactacc tgagagggag aaaaggtacc gtcggaagca
     3181 ttggaatgaa cgtgcaggtc aacaaaaccc atggtaaaac ctcctttgtt tttaaactgt
     3241 tttttctatt ctttctataa taataacacg attgaaatac tttggtaaca cttttaacag
     3301 gaatgtt
//
LOCUS       DBMDUA010000263         3302 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_10050_length_3302_cov_2.750703, whole genome shotgun sequence.
ACCESSION   DBMDUA010000263 DBMDUA010000000
VERSION     DBMDUA010000263.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10050_length_3302_cov_2.750703"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            90..1841
                     /gene="ade"
                     /locus_tag="ACZPCL_13070"
     CDS             90..1841
                     /gene="ade"
                     /locus_tag="ACZPCL_13070"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HIR1163433.1"
                     /translation="MKTCKLLREQVAAATGSKKAELVLKNAQIVNVFTQSVETGDIAI
                     EGGYIVGIGNYEGITEKDLGGAYVCPGFLDGHIHIESSMTSPGEFERAVVPHGTIAVI
                     TDPHEIANVAGTAGIRFMMQSAQKLDLDVYFMLPSCVPATDLDESGAELLARDLEPFY
                     ADEKVLGLAEMMNAFGVTHGDKGCFEKLVQARSLKKAIDGHAPALSGKELNAYVTAGI
                     RSDHECSDFEEAKEKFARGQWIMIRQGTAAKNLKALMGMFEDPYYQRCLLVTDDKHPG
                     DLIRIGHIDAIIREAVSMGADPIRAIRMGTLNAAAYFGLHDMGAVAPGYKADLAVFDD
                     LRTLNVKQVYKGGKLVAENGKMLHQKEKNTDWSTEIKERVFHSFHRVPITVEELQLKE
                     TTGTHQRVIDMVAHELITKERIEEWKELPGVAPGVDISRDIVKLAAIERHKNTGHVGL
                     GFLGKYGLKKGAVATSIGHDSHNLVIAGVTDEDMVLAGNRVIENGGGLAIALEGKVLA
                     DLPLPIGGLMADEPVEVVDEKLEHMKKLSVELGISEDIDAFMTLAFISLPVIPKLRLN
                     TYGVVDVEKHQVVEARF"
     gene            complement(1838..3193)
                     /locus_tag="ACZPCL_13075"
     CDS             complement(1838..3193)
                     /locus_tag="ACZPCL_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1163434.1"
                     /translation="MSSTPSPKSQKMTEGNITKLLITFALPLFVGNLFQQFYNTVDSI
                     VVGNFVSKTALAAVGSTDCIINTIIGFFTGLSTGAGVVIAHSFGSGNDKALHRAVHTT
                     IALTLVLSVVFTIAGLLLSPVFLQLMDTPEDVLPEASQYLRIYFAGISGLMLYNMGSG
                     ILRAVGDSRRPLYILMVCALGNIFFDLLFVIVFHAGVEGVAYATILSQWISAILVLVI
                     LTREKSSYRLIWKDLHIHKGTAMSIFRIGFPAGLQIAVTSFSNVFVQSYINHFGSSCM
                     AGWTTYGKLDKFCLLPIQSIGLAVTTFVGQNLGADNMKRARKGTTVALFISIATALIL
                     MAPVIIFAPFLASLFTNEPEVLTYGIHFIRLMMPFYLAISFNQIFANALRGAGNSVTP
                     MVIMMGSFIVFRQIYLYFISKYFNNITATALGYPLGWVLCSILLIIYYWKVGLNVKKK
                     I"
BASE COUNT          984 a          671 c          863 g          784 t
ORIGIN      
        1 aatgtactcc caggtgcagt attactctta gaaactttac attttagggg aaaagaattc
       61 cgggggtata tacgagagga gggggataca tgaaaacatg taagttgctc agagagcagg
      121 tggcagctgc cacaggaagc aaaaaagcag aactggtgct gaaaaatgca cagatcgtca
      181 atgtatttac ccagtcagtg gaaacagggg acattgccat tgagggcgga tacatagtag
      241 gaattggaaa ttatgaggga ataacagaaa aagatctggg cggagcttat gtgtgtcccg
      301 gctttttaga cggacatatc cacattgaaa gctccatgac ctccccaggg gagtttgaac
      361 gggctgttgt tccacatggc accattgccg tgatcacaga tccccatgag atcgccaatg
      421 tggcaggaac ggcagggatc cggtttatga tgcagtcagc gcagaagctg gatctggatg
      481 tgtattttat gctgccttcc tgtgtgcctg ctaccgattt agacgagtca ggggcagaac
      541 ttctggcaag agatctggag ccattttacg cagatgaaaa ggttctgggc cttgcagaaa
      601 tgatgaatgc ctttggcgtg acccacgggg ataaggggtg ctttgaaaag cttgtacagg
      661 cacgttccct gaaaaaagcc attgatggcc atgcaccggc tctttccgga aaagagctaa
      721 atgcctatgt tactgcagga atccgctctg accatgagtg ttcggatttt gaagaggcaa
      781 aggaaaaatt tgccagaggc cagtggatca tgatccgtca gggaacagcg gcaaagaact
      841 taaaagctct tatgggaatg tttgaggacc cctattatca gcggtgtctg ctggtaacag
      901 atgataagca tccgggagac ttgatccgta ttggtcatat tgatgccatt atcagagaag
      961 cagtttccat gggtgctgat cccatccgtg cgatccgcat gggtacttta aatgcagcag
     1021 cttattttgg actgcatgat atgggcgctg tggcacctgg ttataaggca gatctggcag
     1081 tttttgatga tctgagaact cttaatgtaa aacaggtata caaaggtgga aaactggtgg
     1141 cagaaaatgg aaaaatgctc catcagaaag aaaaaaatac agactggtcc acagagatca
     1201 aagaacgtgt attccattcc ttccacagag ttcctattac agtggaagag ctgcagttaa
     1261 aagaaaccac aggaacccat cagcgggtca ttgacatggt agcccatgaa ctgatcacaa
     1321 aagagcggat cgaagagtgg aaagaactgc caggcgttgc tcccggtgta gatataagcc
     1381 gcgacatcgt aaaactggct gccattgagc gccacaaaaa tacaggccat gtaggactgg
     1441 gatttttagg aaaatacggc ttaaagaaag gtgccgtagc caccagcatc ggacacgatt
     1501 cccacaatct tgtcatcgcc ggagttacag acgaagacat ggtccttgca ggcaatcgtg
     1561 tgatcgaaaa tggaggtggc cttgccattg cccttgaggg caaagtattg gcagatctgc
     1621 cgctgcccat cggcggtctc atggcagacg aaccggtaga agtggtagat gaaaagctgg
     1681 agcatatgaa gaagctttct gtagagctgg gcatttcaga agatattgat gctttcatga
     1741 cactggcatt tatcagcctt ccggttattc caaagctgag actgaataca tatggagtgg
     1801 tagatgtgga gaagcatcag gtggtggaag ccaggtttta gatcttcttt ttcacattta
     1861 atcctacttt ccagtagtaa atgatcagca atatactgca taacacccat cctaaaggat
     1921 atccaagggc ggtagcggta atattattga agtattttga aataaagtaa agatagatct
     1981 ggcggaagac gataaagctt cccatcataa tgaccatagg ggtgacggag tttccggcac
     2041 cccttaatgc gtttgcgaag atctggttga aactgatggc cagatagaat ggcatcatta
     2101 aacggataaa atggataccg taggtcagca cttccggttc attggtgaac agagaagcaa
     2161 ggaatggggc aaaaatgatc acaggtgcca tcaggatcag ggctgttgca atggagatga
     2221 ataatgcaac cgtggttccc tttctggcac gtttcatatt gtcagcgcca aggttctgtc
     2281 ctacaaaggt ggtaactgcc agaccgatac tctggatcgg aagcagacag aacttatcca
     2341 gttttccata ggttgtccag ccagccatgc aggaggagcc gaaatggttg atataggact
     2401 gaacaaatac attggaaaaa gaagtaacag cgatctgaag tcctgcagga aagccaatac
     2461 ggaagataga catggcagtt cctttgtgga tatgcaggtc tttccaaatt aatctgtatg
     2521 agcttttttc ccgcgtaagg atcacaagga ccagtatcgc tgagatccat tgggaaagaa
     2581 tggtagcgta ggcaacacct tcaacacctg cgtgaaaaac gatcacaaaa agcaggtcaa
     2641 aaaagatatt tccaagagcg cagaccataa gaatatataa aggacgtctg gagtcaccca
     2701 cagccctcag gataccggaa cccatattgt aaagcatcag gccggaaata cctgcaaaat
     2761 agatgcgcag atactgggat gcctccggaa gaacatcttc cggtgtatcc attaactgga
     2821 gaaatacagg ggataaaagc agtccggcaa tggtaaagac tacagataat acaagggtca
     2881 gtgcaatggt agtatgtaca gcgcgatgaa gagccttgtc attcccgctg ccaaaactgt
     2941 gggcgatcac tacacctgcg ccggtagaaa gaccggtaaa gaacccgatg atcgtattga
     3001 tgatacagtc tgtggaacca accgctgcca gagctgtttt actgacaaaa tttcccacaa
     3061 caatactgtc aaccgtgtta taaaattgct ggaacaggtt gcctacaaag aggggaaggg
     3121 cgaacgtgat caggagtttg gtgatattgc cttcggtcat tttctggctt ttcggtgatg
     3181 gggtagaaga catggtggtt tgttcctttc tgtagtatgt aggtgatcga cttgatgaca
     3241 gacaaagtac ttttcgaaca ggctttgcca cccgatatcg tacctgtttt atacaaccgc
     3301 tc
//
LOCUS       DBMDUA010000264         3300 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_10057_length_3300_cov_3.886840, whole genome shotgun sequence.
ACCESSION   DBMDUA010000264 DBMDUA010000000
VERSION     DBMDUA010000264.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10057_length_3300_cov_3.886840"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            49..3087
                     /locus_tag="ACZPCL_13080"
     CDS             49..3087
                     /locus_tag="ACZPCL_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR1163435.1"
                     /translation="MTTLPEKGEEIFGYKVMDQTTVSMLGARITELSHACSGARVLYI
                     ENDDRELGFNLIYRTPQLDQSDSNHILEHLMLCSCSRYPSRDIFFDMDSKSYSTFMNG
                     LTDNTYTCYPVCSQSEDQLLKIMDVFLCCMEEPDALKEDYFFRREALRYELESEDEEL
                     SLEGTVFNEDWGHLTDIQENADSFMAETLYEGQTASHLLGRAHFHYKDISFGRIRERF
                     EKFYRYSNCLIVLYGKMDIARVLTFLDKEHLSRFPVAAGIVDHDLLSYFHEPVKQGFF
                     KRQAESPAYEGNPGNNNGIIDYGIDLSGCSEQDLIFWDMVTDILDQDTSAIHRAAREK
                     GLNNVIEVYLDTAPALPSLKFRLHNAKPGQDEEFLAAVKTGLKEVSENGVSSDLFHAV
                     LKENRLSDCLTREAPHLGFHISEEIGKYWSTTDKTGYFTLYENCFDTFFQDEKQDILR
                     RLAGDALTPSLSAVVTTVPKPGLAEAMEEEKEQYLKEKKASLSKKEILKLMEDTKDFQ
                     IWNQNDQCNLDFLIQPEDLPGPEAEPVISETVLHDIHCLSSAVSLKGIGCYQLFFDIS
                     GLKPSDWNYLTLYQMLLTELDTSHFTVEQQKNKEQELLYDCTFDELYPEREAGKNSHP
                     MMSVFWYGLTEDFEEGLELLLDLMGGCDYEDCETILRVIDKYLPDYDMSRSDNGPSLA
                     YSLTERYIRRDSCFRYLLNQPGMYDFLKEIRDVLERAQEVEKSVNSTDYTDMQLSSSH
                     TPGTTETATLDVKALEAAKQISKNLRRVADCILNKHRLVFLACADENSLASILEHSTK
                     RLSALHEVTEDAPLPDSIFTCPRRSAAAIDSPLEEVRMTGDFGNVSEFMGRHLPFLLA
                     AADKYIKPAIRYQGCAYDSGVDFLLPNRYFTLWSTSDSRIRSTLETFASAGEQLENLA
                     ISEEDLRGYILSAYAQALPPSGMLNGRMRFLRRRLFGISTDHINKMIMDIRNSSLKDQ
                     RTAAKLIHKILQNSPAAVVGNEKLIEENKDLFDEVMKLHS"
     gene            complement(3176..>3300)
                     /locus_tag="ACZPCL_13085"
     CDS             complement(3176..>3300)
                     /locus_tag="ACZPCL_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HIR1163436.1"
                     /translation="TDKFSELVQKNVVDWLFGHIKTFDRSVAEFIFMRENPDRC"
BASE COUNT          912 a          711 c          777 g          900 t
ORIGIN      
        1 gaatacgaaa aaagtaattt tgaaactgaa acatcaggag gatcttacat gacaactctg
       61 ccggaaaaag gcgaagagat ttttggatat aaagtgatgg accagaccac tgtttccatg
      121 ttgggagccc gtataacgga actctcccat gcctgcagcg gtgccagagt cctgtatata
      181 gaaaatgatg accgggaact gggttttaac ctgatctacc gcacgcccca gttagaccag
      241 tcggacagca accacatttt agagcatctg atgctgtgct cctgctcccg ctatcccagc
      301 cgggatattt tctttgatat ggacagcaaa agctattcta cctttatgaa cggccttacg
      361 gataatacct acacctgcta tccggtgtgt tcccagagtg aggaccagtt attaaagata
      421 atggatgtat ttttatgctg tatggaggaa ccggatgcct taaaagaaga ttatttcttc
      481 cgtcgggaag cactgcgcta tgagctggaa agtgaagatg aggaactgtc tttagaagga
      541 accgtcttta atgaagactg ggggcattta acggacattc aggaaaatgc agacagcttt
      601 atggcggaaa ctctttatga aggccagact gcttcccatt tacttggccg tgcacatttt
      661 cattacaagg atatttcttt tgggcggatc agggagcggt ttgaaaaatt ttaccgttat
      721 tccaactgcc tgatcgtttt atacggaaaa atggatattg cccgggttct gacttttctg
      781 gacaaagaac atttatcccg ttttccagtt gcagcgggca tagtggatca tgatcttctt
      841 tcttatttcc atgaaccggt aaaacaggga tttttcaaac gtcaggcgga aagtccggct
      901 tatgagggaa atccgggaaa taataacggg atcattgatt atggcatcga cctttccggc
      961 tgcagtgagc aggacctgat tttctgggac atggtaacag atattttgga tcaggacacc
     1021 tctgccattc atcgtgccgc ccgtgaaaag ggtcttaaca atgtgatcga agtttatctg
     1081 gatacagcac cggctctccc ttctttaaaa ttccgcctgc acaatgcaaa accgggacag
     1141 gatgaggagt ttttagctgc tgtaaagaca ggtcttaaag aagtttccga aaatggcgtt
     1201 tcttcagatc tgtttcatgc agttttaaaa gaaaaccgtc tttctgactg tcttaccaga
     1261 gaggcgcctc atcttgggtt ccatatttct gaggagatcg gtaaatactg gagcacaacg
     1321 gataagaccg gatattttac tctttatgaa aactgttttg ataccttctt tcaggatgag
     1381 aaacaggata ttttgcgcag acttgctgga gatgctttaa ctccttcttt aagtgccgtt
     1441 gtcaccactg ttcccaaacc gggtctggcg gaagccatgg aagaagaaaa ggaacagtat
     1501 ttgaaagaaa aaaaggcttc tttaagcaaa aaagagattt taaaattaat ggaggacaca
     1561 aaggatttcc agatctggaa ccagaatgac cagtgcaatc tggactttct catacagccg
     1621 gaagatctgc ccggcccgga agcagagccg gtgatctctg aaaccgtttt acatgatata
     1681 cactgccttt ccagtgcggt ttctttaaag gggatcggct gctatcagct tttctttgat
     1741 atcagcggtt tgaagccttc tgactggaat tacctgaccc tctatcagat gcttttaaca
     1801 gaactggata ccagtcattt tacagtagaa cagcagaaaa acaaggaaca ggaactgctt
     1861 tatgactgta cttttgatga actgtatccg gaaagggaag ctggcaagaa cagtcatcct
     1921 atgatgagcg tgttctggta tggacttaca gaggattttg aagaaggtct ggaactgctt
     1981 ttggatctta tgggcggctg cgattatgag gactgtgaga ccattttacg ggtcattgac
     2041 aagtatctgc cggattatga catgtccagg tctgacaatg gtccatctct ggcttacagc
     2101 cttacagagc ggtatatcag gcgggacagt tgcttccggt atctgctgaa ccagccaggt
     2161 atgtatgact ttttgaagga gatacgggat gtgctggagc gggcgcagga ggttgaaaag
     2221 tcagtcaatt ctactgatta tactgatatg caactcagca gctcacatac cccaggcacc
     2281 actgagactg cgactctgga cgtcaaagcc ctggaagccg caaaacagat ttctaaaaac
     2341 ctgcgccggg tggctgactg cattttaaat aaacacaggc ttgtattcct tgcctgtgca
     2401 gatgaaaaca gcctggcatc tattctggaa catagtacaa aacggctttc agctcttcat
     2461 gaggtcacag aagatgcacc tcttccggac agcattttca cctgcccacg cagaagtgcc
     2521 gctgctattg acagtccttt ggaagaagtg cgtatgaccg gtgattttgg caatgtttct
     2581 gaatttatgg gccgtcatct tccatttttg ctggctgcgg ctgacaaata tattaaacct
     2641 gctatccgct atcagggctg tgcctatgac agcggtgtgg attttctcct gccaaaccgg
     2701 tattttacct tgtggagcac ttccgactca cggatccgct ctactcttga aacattcgcc
     2761 tctgcaggag aacagcttga aaacctggcg atcagtgagg aagacctgcg gggctatatt
     2821 ttaagcgcct atgcccaggc actgccaccg tccggcatgt taaacggccg gatgcgtttc
     2881 ctgcgccgca gattatttgg tatttcaacg gatcatatca ataaaatgat aatggatatc
     2941 aggaacagtt ctttgaaaga ccaaagaacg gctgccaagc tgatccacaa gatcttgcag
     3001 aacagtccgg ctgccgttgt aggaaatgaa aagttgatcg aggaaaataa agatttattt
     3061 gatgaagtga tgaagttgca ttcataaaaa tttatagaag ctgccactgg cagcctttgc
     3121 cagatccata gcaacataac aggggacgca ttactcacgc gtcccctgat ctaatttaac
     3181 atctgtctgg attttctctc ataaagataa attctgctac agaacggtca aatgtcttaa
     3241 tatgtccaaa cagccagtca actacgttct tctgtaccag ttcactgaac ttgtctgtag
//
LOCUS       DBMDUA010000265         3268 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_10185_length_3268_cov_2.874329, whole genome shotgun sequence.
ACCESSION   DBMDUA010000265 DBMDUA010000000
VERSION     DBMDUA010000265.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10185_length_3268_cov_2.874329"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            67..1656
                     /locus_tag="ACZPCL_13090"
     CDS             67..1656
                     /locus_tag="ACZPCL_13090"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1163437.1"
                     /translation="MKELKKSTRREPVSRKKNTAEKKEQTAKKSTKKNTGKEIKKENK
                     KDTEKGTWKSVTKERVYDPNGKVLVITYACVVLFLALAVYMGYFLQMKSEDVINNPYN
                     ARLDSFSDRIVRGSILASDGTVLAETTTDDAGNETRVYNYGGVFDHAVGYSSKGKTGI
                     EAMANFYLLSSHVNLVEQAGNELAGAKNLGDSVVTTLDMELQQAAYAALGDRRGAVIA
                     MEPDTGKILAMVSKPGYDPNTLLQDWASLTDSSNNQGQLVNRATAGLYPPGSTFKIVT
                     ALEYMREHPDDYKDFHFDCNGVYHNGDYSIKCFHSEAHGSQDFMKAFANSCNGAFSSL
                     GLTMDLDRFHATAEELLFNNPLPLSGYSYKESSFNMKGGAGTWEVLQTSIGQGTTLIT
                     PLHNALITAAVANGGTLMKPYLLDRVVSAGGEEVKKFLPTSGGTLMTATEAANLTELM
                     RDVVVEGTGSSVRTDAYTVAAKTGTAEFETGKETHAWFTGFAPAESPKIVVTVLVEES
                     GSGGKIAGPIARQLFDIYMGR"
     gene            1808..2602
                     /locus_tag="ACZPCL_13095"
     CDS             1808..2602
                     /locus_tag="ACZPCL_13095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR1163438.1"
                     /translation="MFRADDYVKVSDLAEAYELCQKRSSLVVGGMVWMKMTHITKRTI
                     VDLSGLGLDAIEEKEGEFSIGCMCSLRQLETHEGLNRYFDGIFRECTRNIVGVQMRNC
                     ATVGGSIFARFGFSDILTCLLALDTYVELYHEGTIPLSEFAARPVRRDQKDILVRIII
                     KKDGRKAAYTSQRNSRTDFPVIACCVSNLGNKWFVSVGARPAKAKAVIITEDDFDTLK
                     EMAKEAAEAFTYGDNTRASGAYRKTLATVYTRRLMEQIKGRSVGIV"
     gene            2599..3066
                     /locus_tag="ACZPCL_13100"
     CDS             2599..3066
                     /locus_tag="ACZPCL_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR1163439.1"
                     /translation="MRLSFRLNGKQVETDVRPDMLLLDLVRDLGCYSVKRGCETANCG
                     LCTVWLDGKPVLSCSTLAIRADGRHVTTLEGLQKEAEEFGMFLAREGGEQCGFCNPGF
                     IMNVLAMEKELKDPDEEDIKEYLSGNLCRCSGYMSQLRAVKKYLDMKQSGKEA"
     gene            3071..>3268
                     /locus_tag="ACZPCL_13105"
     CDS             3071..>3268
                     /locus_tag="ACZPCL_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163440.1"
                     /translation="MAYTQINPATGKKFRLIGAPVVKKDAKALVTGKPVYTDDLALKD
                     CLIVKVLRSPHAHALIEEIDCK"
BASE COUNT          989 a          607 c          876 g          796 t
ORIGIN      
        1 ccaggggctt tatatcatca agcggaatga tgaagaagag atggaagaaa tggaggcgca
       61 ggaagaatga aagaactgaa aaaaagtacg cgaagagaac ctgtctccag aaagaaaaat
      121 acggctgaaa agaaggaaca gacagcaaag aagagtacaa aaaaaaatac aggtaaagag
      181 ataaagaaag aaaacaaaaa agatacagaa aaaggtacct ggaagtcagt gacaaaagag
      241 cgggtatatg atcccaatgg gaaagtgctg gtgatcactt atgcatgtgt ggtcctgttt
      301 ttagcactgg ctgtatacat gggatatttt ctgcagatga aaagcgaaga tgtgatcaac
      361 aatccttaca atgccagact ggacagcttt tcagaccgca ttgtaagagg cagtatcctg
      421 gcatcagatg gaacggtgct ggcagagact accactgatg atgcgggaaa tgaaacaagg
      481 gtttacaatt atggaggtgt gtttgaccat gcggtaggct attcctctaa aggaaaaact
      541 ggcattgaag ccatggcaaa cttctatctt ttaagttctc atgtcaatct ggtagagcag
      601 gcgggaaatg agctggcagg tgcgaaaaat ctgggagaca gcgtggttac cactctggat
      661 atggaactgc agcaggcagc gtatgcagct ttaggagaca ggagaggagc ggtgatcgcc
      721 atggaaccgg atacaggaaa gatcctggca atggtttcca agccgggtta cgatccaaat
      781 acccttcttc aggactgggc gtctcttact gacagttcca ataaccaggg gcagctggta
      841 aaccgtgcaa ctgccggact atatcctcct ggatcgacct ttaagatcgt gacggctctt
      901 gagtatatga gggagcatcc tgacgattac aaggattttc attttgactg taatggcgtt
      961 tatcataatg gagactactc cattaaatgt ttccacagtg aagcccatgg aagccaggat
     1021 tttatgaagg cttttgccaa ttcctgtaac ggggcatttt ccagcctggg tctgaccatg
     1081 gatctggacc ggttccatgc tacggcggag gaattgctat ttaataatcc gttgccatta
     1141 tccggctact cctataagga aagttctttt aatatgaaag gaggcgccgg aacctgggaa
     1201 gttttacaga cttccattgg ccagggaact acactgatca cgcctcttca caatgcgctg
     1261 atcactgcgg ctgttgcaaa tggtggaacc ctgatgaaac catatttgct ggaccgagta
     1321 gtcagtgccg gcggggaaga agtgaaaaaa ttccttccga cttcaggggg aactcttatg
     1381 acagccacag aggcagcaaa tctgacagag ctgatgcgtg atgtagtggt ggaaggaacg
     1441 ggttcttctg tgcgcaccga tgcctataca gtagctgcaa aaacgggaac tgcggagttt
     1501 gaaacaggaa aagagacaca tgcatggttc accggttttg caccggctga gtcaccaaag
     1561 atcgttgtta cggtcctggt agaagagtcc ggaagcggtg gaaaaatcgc aggacctatt
     1621 gcaagacagt tgtttgatat atatatgggg agataatctt taaaaagaat aacgttttct
     1681 atggaaaaca ggcagatttc gtgttataat ctgcctgttt tgttttgaat tctactaaaa
     1741 gacgaactat ttattagaaa aacttatcaa tatttattag cacaaacata cgaaggggga
     1801 aaaacaggtg ttccgagcag atgattatgt taaggtaagc gatctggcag aggcatatga
     1861 gctttgtcag aagcgaagca gtctggtagt aggcggaatg gtatggatga aaatgactca
     1921 tattaccaag cgtactattg tagatttgtc cgggcttgga ctggatgcaa tcgaagaaaa
     1981 agaaggagag ttttccattg gctgtatgtg cagtttacgc cagttagaga cccatgaggg
     2041 gctgaaccgg tattttgatg gtattttcag ggaatgtacc agaaatatcg ttggcgtgca
     2101 gatgagaaac tgcgctacgg taggaggcag tatttttgcc cgtttcggat tttccgatat
     2161 actgacctgc cttctggcac tggatactta tgtggagctg tatcatgagg gaaccattcc
     2221 tctttctgaa tttgcagcca gacctgtaag aagggatcag aaggatatcc tggttcgtat
     2281 tattataaag aaagacggaa gaaaggctgc ttataccagt cagcgcaatt cccgtaccga
     2341 ttttccggtg atcgcctgtt gtgtttccaa ccttggaaac aaatggtttg tgtccgttgg
     2401 ggcaagacct gcaaaagcaa aggcagtgat cattacagag gatgattttg ataccttaaa
     2461 agagatggca aaggaagcgg cagaagcatt tacttatggc gataatacac gcgccagcgg
     2521 tgcttacaga aagactctgg caactgttta cacccgccgt cttatggagc agatcaaagg
     2581 aaggagcgtg ggcatagtat gagactttca tttcgcttaa atggaaaaca ggtagagaca
     2641 gatgtaagac cggatatgct gttattggat ctggtacgtg atctgggttg ctacagcgta
     2701 aaaagaggct gtgagacagc aaactgcggc ctttgtactg tatggctgga cggaaagccg
     2761 gttctgtcat gttctacact tgcgatccgg gcagatggcc gccatgtgac tacactggaa
     2821 ggtctgcaga aagaggcaga agaatttggt atgtttttag caagagaagg cggagagcag
     2881 tgcggtttct gcaatccagg atttattatg aacgttcttg ctatggaaaa agagttaaaa
     2941 gatccagatg aagaggatat taaggaatat ctttccggaa atttatgccg ctgcagcgga
     3001 tatatgtccc agctgcgtgc tgtaaaaaaa tatctggata tgaagcagag cggaaaggag
     3061 gcataaggaa atggcatata cacagattaa tcctgctaca ggaaagaaat tccgtctgat
     3121 cggcgctcct gttgtaaaga aagacgcaaa ggcactggta acaggtaagc cggtgtatac
     3181 tgatgacctg gcattaaagg actgcctgat cgtaaaagtg cttagaagcc cacatgccca
     3241 tgcactgatc gaagagatcg actgcaag
//
LOCUS       DBMDUA010000266         3263 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_10197_length_3263_cov_2.100253, whole genome shotgun sequence.
ACCESSION   DBMDUA010000266 DBMDUA010000000
VERSION     DBMDUA010000266.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10197_length_3263_cov_2.100253"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(219..1262)
                     /locus_tag="ACZPCL_13110"
     CDS             complement(219..1262)
                     /locus_tag="ACZPCL_13110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR1163441.1"
                     /translation="MREKWFREHIWWMSFILSLMVVWVHSENVDLFLGEIGRESLVYR
                     LEFFFAQTLGQIAVPGFFMISAYLFYRNFQFSKTVSKWKSRCKSLLLPYVLWNILYYL
                     GYVVVTRLSFVKKIIGKEPVAFGLKELFQAVAYYKYNPVFWYLFQLLLLVVLAPFLYW
                     IICRKWSGLLWLLLLITALWKNVSLPILNLDALFYYSAAGYAVLFSEKVEGENTSSGK
                     KIWFAILILCWLTLWFLGRPGATLHFTPVHTVLTRLLGVLIFYSILKEIPFRPATSTV
                     KNSFFLYAIHFPWARFFNKAGAVLFKGNQGAAAVFFLTMPFLILGISKVLGELLKHTV
                     PGIYEILSGGRGQ"
     gene            complement(1577..2779)
                     /locus_tag="ACZPCL_13115"
     CDS             complement(1577..2779)
                     /locus_tag="ACZPCL_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009220153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR1163442.1"
                     /translation="MLYEEKIIETIPVGPLGLIPLKSCTGLGKKVDDYLVEWRRERES
                     EHKSTIAFSGYQRDSYILEANTPRFGSGEGKGTIEESVRGDDLYIMVDVCNYSLTYSL
                     FGMTNHMSPDDHFQDLKRIIAAAAGKARRINVIMPFLYESRQHKRSGRESLDCAMALQ
                     ELVNMGVENIITFDAHDPRVQNSIPLKGFETVQPIYQFLKHLLKNEPDLQIDSDHMMV
                     ISPDEGGTSRAIYFANMLGLDMGMFYKRRDYTKIVNGRNPIVAHEFLGSSVEGKDVLI
                     IDDMISSGESMLDVAKELKRRKARKVFICATFGLFTGGLAKFDQYYQDGLIDRILTTN
                     LVYQTPDLLSRPYYIDVDMSKYIALIIDNLNHDASLSDLLTPTKRINKLLERYKEQQA
                     AKNSESNA"
     gene            complement(2887..>3263)
                     /locus_tag="ACZPCL_13120"
     CDS             complement(2887..>3263)
                     /locus_tag="ACZPCL_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1163443.1"
                     /translation="CIARKLMDTYHSVIYFSASDLFEVFSRNKFDYDQAEEMKDTYRY
                     ILDCDLLIIDDLGTELNNSFTSSQLFYCINERMNMNRSTIISTNLSLTQLRDSYTDRV
                     TSRIMRYRIIPLYGRDIRLLKR"
BASE COUNT          962 a          710 c          630 g          961 t
ORIGIN      
        1 tctctaatct gtgcttttac tgaatccaca agctgctgat acaatggagt agaatattct
       61 ttattaatag ccatatcatt ccctcatttc tgtccttgta acaagaacat agttgttata
      121 atacatctta tactgatttt aacatattca taaaaaaagt acaacctttc atctttctta
      181 tataaaaaat ttatcatcaa tgcacaatat cagacatttt actgtccgcg gccgccggaa
      241 agaatttcat aaattccggg aactgtatgt tttaacagct ctcccaggac cttgcttata
      301 ccaaggatca gaaaaggcat agtcaggaaa aatactgctg cagccccctg atttccttta
      361 aaaagtacag ctccggcttt gttaaagaaa cgggcccatg gaaaatggat ggcataaagg
      421 aaaaagctgt tttttactgt agaagtggct ggtctgaagg gtatttcttt taaaatactg
      481 tagaaaataa gcactcccag taaacgggtc agaacagtat ggaccggtgt aaaatgtaaa
      541 gttgctccag gtcttccaag aaaccatagg gtaagccagc aaagaataag aatggcaaac
      601 cagatttttt tcccggaaga tgtattttct ccttccacct tttcagaaaa aagcacagca
      661 tacccggctg cactgtagta gaacagagca tccagattta agatgggtaa acttacattt
      721 ttccacagag cagttataag aaggagcaac cataacagac cgctccattt ccggcagatg
      781 atccagtaca gaaaaggcgc cagaaccact aaaagcagca gctggaacag ataccagaac
      841 acgggattat atttataata tgctaccgcc tgaaacagtt cttttaaacc aaaagcaacc
      901 ggctcttttc caatgatctt tttcacaaaa gaaagccttg tgactacaac gtaacccaga
      961 taataaagaa tattccacaa aacataagga agaagaaggc ttttgcaccg gctcttccat
     1021 ttggaaactg tttttgaaaa ctggaaattt ctgtaaaaaa gataggcaga gatcatgaaa
     1081 aagccgggaa ctgcgatctg tcctaaggtc tgagcgaaaa agaactccag cctgtaaacc
     1141 aggctttccc ttcctatttc acctaaaaac agatcaacat tttctgaatg gacccataca
     1201 accatcaggc tgaggataaa actcatccac cagatatgtt cccgaaacca tttttccctc
     1261 atatatcttt tcccaacttt cccacatatt ttgatatgct cttgcgcatt acatcttgat
     1321 gtaccctcaa aatctttttg tgcttgatct tgcgagaaaa ttttttgtca gttgtgccca
     1381 aatttctgag gcgtacgcgg cgcgtacgtt gatgaaattc gggtacaact ggcggaaaat
     1441 tggccgcaaa agcaggtgca ggaaaggttt tgagggtaca tcctaataaa aagggctgcg
     1501 caaaacagct tttattgtct gctattttgc cacagcccct gtaatgtttt attctttact
     1561 ggatcagcgc cccaaatcaa gcgttacttt cgctgttttt agctgcctgc tgctctttat
     1621 atctttcaag aagcttgttg atcctcttgg taggtgtcag cagatcgctt aaggatgcgt
     1681 catggttcag gttatcaatg ataagggcaa tgtacttgct catatctaca tcaatatagt
     1741 atggtctgct taacagatct ggtgtctggt ataccaggtt ggtagtaagg atacggtcaa
     1801 tgagaccatc ctgataatac tggtcaaact ttgcaagtcc gccggtgaac agaccgaaag
     1861 tagcacagat aaatactttt cttgcttttc tgcgctttaa ttcctttgca acatctaaca
     1921 tgctctcgcc ggaagagatc atatcatcaa tgatcagtac gtcttttcct tctacgctgg
     1981 aacctaagaa ctcatgagca acgatcgggt ttctgccgtt tacgatcttg gtgtaatcac
     2041 ggcgcttata gaacataccc atatccagtc ccagcatatt tgcgaagtag atggcacggc
     2101 tggtaccgcc ttcatccggg ctgatgacca tcatgtggtc gctgtcgatc tgcagatccg
     2161 gctcattctt taacagatgc tttaagaact gatagatcgg ctgtacagtc tcaaatccct
     2221 ttaacggaat tgaattctgt actcttgggt catgtgcatc gaaagtaatg atgttttcaa
     2281 cacccatatt tacaagttcc tgaagagcca ttgcacagtc taaggactct cttccggaac
     2341 gcttatgctg acggctttca tacaggaatg gcatgataac gttaattctg cgtgccttgc
     2401 ctgctgctgc agcgataatt ctcttcagat cctggaaatg atcatctgga gacatatgat
     2461 ttgtcattcc aaacagtgaa taggtcagac tgtaattaca tacatctacc ataatataga
     2521 ggtcatcacc tctgacagat tcttcaattg ttccttttcc ttcaccggaa ccaaaccttg
     2581 gtgtgtttgc ttccagaata taggaatctc tctgatatcc ggaaaatgcg atagttgact
     2641 tatgttcgct ttctctctct cttctccact ctaccagata atcgtccacc ttttttccaa
     2701 gacctgtgca gctctttaaa ggaataaggc ctaaaggacc taccggaatt gtttcaatga
     2761 ttttttcctc atataacatg atatttatcc tcctgtttta accggcattg ccttgttatc
     2821 tcatacgaat gccagcatat atctttatac catttttcgt ttgtatccgt caagatatct
     2881 taagggttat ctttttaaca gacggatatc cctgccgtaa agagggataa tacggtaacg
     2941 catgatacgg gaagtcacac ggtccgtata ggagtcacgc agctgggtca gggaaagatt
     3001 ggtggaaatg atagtggaac ggttcatatt cattctctca ttgatgcagt aaaataactg
     3061 ggaggaagta aaactgttgt ttaattcggt tcccagatca tcaatgatca gaaggtcaca
     3121 atccaggata taccggtaag tgtccttcat ctcttctgcc tgatcataat caaatttgtt
     3181 tcttgaaaac acttcaaaga gatcactggc agaaaaatag atcacggaat gataggtatc
     3241 catcagcttt ctggcaatgc agt
//
LOCUS       DBMDUA010000267         3245 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_10263_length_3245_cov_2.268766, whole genome shotgun sequence.
ACCESSION   DBMDUA010000267 DBMDUA010000000
VERSION     DBMDUA010000267.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10263_length_3245_cov_2.268766"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     regulatory      complement(2..77)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1092..2090)
                     /locus_tag="ACZPCL_13125"
     CDS             complement(1092..2090)
                     /locus_tag="ACZPCL_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HIR1163444.1"
                     /translation="MTAKKFNLKDFLINNGIIVVLVLLAIYTGIMQPTFVSGSNLKNI
                     ALNVAPRFIIACGVSGCLITKGTDLSAGRMVGLSACLAGTLLQKADYSGKFFPNMPFY
                     GVWWVAAVLLICVAVCAIFGAINGIVVSYLQVPAFIGTLGMQLIVYGVCLVYTNATPI
                     GGYRAAYTEVAKGQLFGFIPYLFLIALAVGIVMWFVYNKMPHGKYMYAIGGNEQAAEV
                     SGVNTKKTKIIIYITAAACYALGGFLVGAKSGGSSVNMGYGWELEAIAACTIGGVSVN
                     GGIGRISGVLIGVLVFEILKTCLQYLGVDTNYQYIAQGIVIVVAIALDIRKYIAKK"
     gene            complement(2103..>2389)
                     /locus_tag="ACZPCL_13130"
     CDS             complement(2103..>2389)
                     /locus_tag="ACZPCL_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1163445.1"
                     /translation="NNPDILIMDEPTRGIDVGAKYEIYQIMIDLVKQGKSIIMISSEM
                     PELIGMSNRIIVMCNGHITGEVEGEEATQERIMSFATQFDLQGKSTTESK"
     assembly_gap    2390..2399
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2400..>3245)
                     /locus_tag="ACZPCL_13135"
     CDS             complement(<2400..>3245)
                     /locus_tag="ACZPCL_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009429878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1163446.1"
                     /translation="VEIAKAVSQHAKIVILDEPTSSLTDNEVEALFRIIRDLKSRGVS
                     MIYISHKMAEIRQIADDITIMRDGTYVGSWEVKDISDDEIVKQMVGRELTNVYPPKED
                     YRTDETILKVEHLCSIHERSFQDCSFELKRGEILGFGGLVGAQRTEMMEAIFGMRHIA
                     SGTVEVLGKQVNIKKPEDAIDNSIGMITEDRRGTGILGCLSINDNTAIASYKNYTNNG
                     VIDQKKVDAVVKDSIQKLNIKTPSGKTLIQSLSGGNQQKVIIARWLANNPDIFFIEKG
                     IARAY"
BASE COUNT          874 a          797 c          612 g          952 t
ORIGIN      
        1 acgtggcaac tggctaccat tttacaggcc ctatagcttt gcgtcccaga ctttcatctg
       61 gtttgccgat ttccttatta taacttttaa acgtatgcaa aaaactgatt ttattatact
      121 atagcactct actgtacaca agaaaaaaca ttaccgcttt aacgctaaaa ataaaaaaag
      181 aacagccgat atatacattc gaactattct cctacttccg aagtccatgt agcattgaca
      241 ttactgtttc ctgctcattt gtactcaaat cgttccatat ccttaaaact tctttctaat
      301 cttctgtcag gttctctgaa ttaccttcct gaaaaaagtt tgtcatataa aaatttttaa
      361 cgtcaaacaa aaaattggaa gccacagtgt tatccatgac ctccgactca ttctgcttat
      421 tctacactgt ccttacaccc aaatcagccc ctctctccat cttatcttgt ccctgctttc
      481 cctggataat tttcttattg atttccaagc gttcatggat tgacgttttc ttctcttttc
      541 ctgtcattgc tctcttttgt gctactggct ttctttcagc tttccctgcg tccttaactg
      601 ctgtatttgc ttcaacgccg gctcttgaca cacgcagacc cgggcacggg ctgcatggag
      661 tttgaaattc gcaaggggcg gttctccttt tggccgaccg ccccatacag cgcagaacgc
      721 cgctgggcgg caagcgaagc agcgaaaaaa cgaataaaag ggggttttta tattgccatt
      781 cgtaaaattt gttgtatatg acttatgatt atcgtgtttg ctcttaaatt tcggataacc
      841 tgcatgttcc ttgttcccat gccacaagta tataacaatt ttccactttt ggctacctta
      901 acccaccgtc taaagccagt ggaattgcgg tagccttact ttcaaaaaat gcaagtgcca
      961 gctcacagaa cctgtcaccg gcagcatttt tccatacatg acagaaaaaa tatgggcacc
     1021 agatccggtc aatcacagat ctggcgccca gccaaaaata ttttatgctt tagaatatta
     1081 tgagtcagta attacttctt agcaatgtac tttctgatat ccagtgcgat cgcaactacg
     1141 ataacgatac cctgtgcaat gtactggtag tttgtatcaa cacccagata ctgtaagcag
     1201 gtctttaaga tctcaaatac cagaacgccg ataagaacac cggagatacg accaatacca
     1261 ccgtttacag atacaccgcc gatggtgcag gctgcgattg cttccagctc ccagccgtaa
     1321 cccatattta cagaggaacc accggattta gcacctacca ggaaaccgcc cagtgcgtag
     1381 catgctgctg ctgtgatata gatgatgatc ttggttttct ttgtatttac gccggaaact
     1441 tctgctgcct gctcatttcc accgatagcg tacatatatt tgccgtgagg catcttgtta
     1501 tatacaaacc acattacaat accaactgct aaagcgatca ggaacagata agggataaaa
     1561 ccaaataact gtccttttgc aacctctgtg taagcagcac gataaccacc aataggagtt
     1621 gcgttggtat aaaccaggca gacaccgtat acgattagct gcatacctaa ggttccgatg
     1681 aatgcaggaa cctgcagata agaaactacg ataccgttga tagcaccaaa gatagcacat
     1741 acagctacac agataagcag tacagctgct acccaccata caccataaaa tggcatgtta
     1801 gggaagaact tgccgctgta atcagccttc tgaagtaaag tacctgccag gcaggcagat
     1861 aaacctacca tacgtcctgc agaaaggtca gtacctttgg tgatcagaca cccggatacg
     1921 ccgcaggcga tgatgaaacg tggtgctacg ttaagtgcaa tattttttaa gttggagccg
     1981 gatacgaatg tcggctgcat gataccggtg taaatagcta ataacaccag aacgactata
     2041 attccgttat taataaggaa atcctttaaa ttaaattttt tcgctgtcat ggcttaatcc
     2101 tcctacttgg actctgtggt tgattttccc tgaagatcaa actgtgtcgc aaaggacata
     2161 attctctcct gggttgcttc ttccccttct acttcgccgg tgatatgtcc gttacacatt
     2221 acaatgatac ggttggacat accgatcagc tccggcattt cagaagagat catgataatg
     2281 gacttgccct gttttaccag gtcgatcatg atctgataga tctcatactt ggcacctacg
     2341 tcaatacctc ttgtaggctc atccatgatc aggatatccg ggttgtttgn nnnnnnnnna
     2401 tgtaagctct ggcaatccct ttctcgatga aaaagatatc cgggttgttt gccagccagc
     2461 gggcgatgat aaccttctgc tgattaccac cggaaagaga ctggatcagt gtctttccac
     2521 taggagtctt gatgttcagc ttctggatgc tgtcttttac aactgcatct actttcttct
     2581 gatcgatcac accgttgttt gtatagttct tataagaagc gatcgcagtg ttatcgttga
     2641 tgcttaagca gcctaagata ccagttccac gacgatcctc tgtgatcata ccaatggaat
     2701 tatcaatagc atcttccggt ttcttgatat taacctgctt gccaaggact tctaccgtac
     2761 cgctggcaat atgtctcata ccgaagattg cttccatcat ctctgttctc tgtgcgccta
     2821 ccagaccgcc gaatccaagg atctcgcctc tctttaactc aaaggaacaa tcctggaagg
     2881 aacgttcatg aatgctgcac aggtgctcta ccttcagaat cgtctcatct gttctgtaat
     2941 cttcctttgg aggatataca ttggtcagct cacgtccaac catctgtttt acgatctcat
     3001 catcggaaat atcctttact tcccaggatc ctacatatgt accatctcgc ataatagtaa
     3061 tatcgtctgc gatctgacgg atctcagcca tcttatggct gatatatatc atagaaacgc
     3121 ctctggactt taagtccctg ataatgcgga acagcgcttc tacttcatta tcagtcagag
     3181 aggatgtagg ctcatccagg atcacgatct ttgcgtgctg actgactgcc tttgcgatct
     3241 ctact
//
LOCUS       DBMDUA010000268         3240 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_10289_length_3240_cov_2.186684, whole genome shotgun sequence.
ACCESSION   DBMDUA010000268 DBMDUA010000000
VERSION     DBMDUA010000268.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10289_length_3240_cov_2.186684"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(100..318)
                     /locus_tag="ACZPCL_13140"
                     /pseudo
     CDS             complement(100..318)
                     /locus_tag="ACZPCL_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
     gene            complement(320..1105)
                     /locus_tag="ACZPCL_13145"
     CDS             complement(320..1105)
                     /locus_tag="ACZPCL_13145"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1163447.1"
                     /translation="MKIAVITGASSGMGWECAIRLWEQFPGLDEIWAVGRRKERLVKL
                     EEKVNIPVRKFVLDLSQEKEREILKAELERLKPQVKFLVNAAGFGKIGNVCDLPEKDQ
                     SGMVQVNCTALTAVTTMVLPFMPQNSRILQFASSAAFLPQPGFAVYAASKAYVLSYSR
                     ALNRELKGRKIFVTAICPGPVRTEFFDIAQTTGEIPLYKKLVMAAPGKVVAKAIRDSI
                     AGREISVYGLFMNAFWILAKVFPHSLILDMMEKIEYLPGKDRW"
     gene            complement(1108..3159)
                     /locus_tag="ACZPCL_13150"
     CDS             complement(1108..3159)
                     /locus_tag="ACZPCL_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HIR1163448.1"
                     /translation="MVHDYLPINREDMKKRGWEQCDFVYVSGDAYVDHPSFGTAIISR
                     LLEAHGYKVGIIAQPDWKDNKSIQILGRPRLGFLVSAGNMDSMVNHYSVSKKRRKEDS
                     YTPGGVMGKRPDYAVVVYCNLIRSAYKDVPIIAGGIEASLRRLAHYDYWSNKMKRSIL
                     LDAQADIISYGMGEHSIVELADALDSGLDIKDITFIDGTVYKTKSLESVYDYKLLPDY
                     TELLEDKKRYAESFFVQYSNTDPFSGKRLVEPYEGKVYVVQNPPAKPLTQDEMDDVYA
                     LPYMRSYHPSYEAAGGVPAIREIKFSLISNRGCFGACSFCALTFHQGRIIQARSHESL
                     VDEAKLLTEEPDFKGYIHDVGGPTANFRFPACEKQLTKGACPNKQCLFPEPCKNLRAD
                     HSDYISLLRKLRAIPKVKKVFIRSGIRFDYLLKDPGKKFLRELCEYHVSGQLKVAPEH
                     VADKVLMRMGKPKNSVYREFVKEYKQMNEKLGKDQYLVPYLMSSHPGSSMKEAVELAE
                     YLRDLGYMPEQVQDFYPTPSTISTCMYYTGYDCRTMEKVYVPVNPHEKAMQRALIQYR
                     NPKNYDLVAEALKIAGRTDLIGFDKKCLIKPRNFDKSAHDEKEFRGHGGVSGGKNAVG
                     RNENGRNGNNRNTAGKNTGGRSAGASGRNMEKLSGSKVNAAKTAGKKKGIRNVHKKKG
                     N"
BASE COUNT          778 a          867 c          613 g          982 t
ORIGIN      
        1 tcaatcctga tttttctgcc tgctcccttt gtagtttttc ccggctttac ccgaatttct
       61 gtctttttct tttcctaatg ctgccagttc tggaagaggc gccgaaaact tttcaatggt
      121 ctcatctgcc agaaacagac tgatctcgtc cccttcttcc acccgttcag aaccatcaca
      181 cttctttcca tttaaggtaa tgtttttctt tcgcatcatc ttataaagaa agccttttcc
      241 tgcctggctt aaatatttgg acagcagctt atccagcctc tgtcctgcct cattggaggc
      301 cactttcagt ttctgcattt taccaacgat cctttcccgg taagtactct atcttttcca
      361 tcatatctaa gatcaggctg tgtggaaaca cttttgcaag gatccagaat gcattcataa
      421 aaagcccata gacagaaatt tctcttcctg caatgctgtc ccggatcgct tttgccacaa
      481 cctttccagg tgctgccata accagttttt tgtataatgg aatttcacct gttgtctgtg
      541 caatatcaaa aaattctgtg cgcaccggtc ccgggcagat ggcagttaca aatatctttc
      601 tgccctttaa ttccctgttt aatgcccggc tgtagcttaa cacataggcc tttgaagctg
      661 catatactgc aaagcccggc tggggcagaa aggctgcact ggaagcaaac tgcaggatgc
      721 ggctgttctg aggcataaaa ggaaggacca tggtggtaac agctgtcaga gcagtacagt
      781 ttacctgcac cattcctgac tggtcttttt caggcagatc acatacattt ccgatctttc
      841 caaaaccagc tgcatttacc agaaatttca cctggggttt caagcgttct aactcagctt
      901 ttaagatctc ccgttctttt tcctgactca gatccagaac aaatttgcgc accggaatat
      961 ttactttttc ttccagtttt accaggcgct cttttcgtct tcccactgcc cagatctcat
     1021 ccagtcctgg aaactgctcc cataagcgga tggcacattc ccagcccatt ccagaagatg
     1081 ctcctgttat aacagcaatt ttcattgtca gtttcctttc tttttatgaa cgtttcgtat
     1141 gccttttttc ttgcctgcag ttttcgctgc attcaccttg cttcctgaca gcttttccat
     1201 atttctgccg cttgcacctg cacttcttcc gccagtattt tttcctgctg tattcctgtt
     1261 atttccattc ctgccgtttt catttctgcc aaccgcattc ttgccgccgg aaactccccc
     1321 atgtccacga aactctttct catcatgagc gctcttatca aaattcctcg gcttgatcag
     1381 acatttctta tcaaagccga tcagatccgt tcttccggca atcttaagag cctctgccac
     1441 caggtcataa tttttcgggt tccggtactg gatcagcgct ctctgcatag ccttctcatg
     1501 aggattaaca ggcacataca ccttttccat ggtacggcag tcatatcctg tataatacat
     1561 gcaggtggaa atggttgacg gtgtcggata aaagtcctgt acctgctccg gcatgtagcc
     1621 cagatctctt aaatactccg ccagctccac cgcctctttc atagaagaac ctggatggga
     1681 agacatcaga taaggcacca gatactggtc ctttcccagc ttctcattca tctgcttgta
     1741 ttcttttaca aactctctgt atacactgtt tttcggcttg cccatgcgca tgagcacctt
     1801 atctgccaca tgctccggcg caactttcag ctggccgctt acatggtatt cgcaaagttc
     1861 ccgtaaaaac ttctttcccg gatcctttaa cagatagtca aaacggatac cggaacggat
     1921 gaatactttc tttacctttg ggatcgcacg cagctttctt aagagtgaaa tataatcact
     1981 gtggtccgca cgcaggttct tacatggttc agggaacaga cactgcttat tagggcaggc
     2041 acccttagtc agctgttttt cgcaggcagg gaaacggaaa ttggcagtag gtccgccaac
     2101 atcatggata tatcccttaa aatcaggctc ttctgtaaga agttttgcct catccaccaa
     2161 tgactcatgg cttcttgcct ggatgatcct tccctgatgg aaagtcagtg cgcagaagct
     2221 gcatgcacca aaacagcctc tgttgctgat taggctgaat ttgatctcac ggatagccgg
     2281 gacgccaccg gctgcctcat aagaaggatg atagcttctc atatatggca gagcatacac
     2341 atcatccatc tcatcctgtg tcagtggctt tgcaggcggg ttctgtacca cataaacttt
     2401 gccctcataa ggttccacca gacgttttcc ggaaaacgga tctgtattgc tgtactgtac
     2461 aaaaaagctt tctgcgtacc gtttcttatc ttctaacagc tctgtatagt ccggaagcag
     2521 cttataatca taaacagact ccaggctctt tgtcttataa accgtaccgt ctataaaagt
     2581 aatatcctta atatccagtc cgctgtccaa tgcatctgcc agttccacaa tggaatgttc
     2641 tcccattcca taggaaataa tatctgcctg ggcatccagc aaaatggacc gcttcatctt
     2701 attggaccag taatcataat gagccagacg gcgaaggctt gcctcaatac ctccggcaat
     2761 gataggcaca tctttataag cagaacggat caggttgcag tataccacca ctgcatagtc
     2821 cggtcttttt cccattaccc caccaggagt ataggagtcc tcttttctgc gctttttgga
     2881 tacggaataa tggtttacca tggaatccat atttcccgca gacaccaaaa atcccagtct
     2941 tggccgtcct aagatctgga tgctcttatt gtccttccag tccggctggg caatgatacc
     3001 taccttataa ccatgtgctt ccaacagcct tgaaataatg gccgttccaa aagaaggatg
     3061 gtctacatag gcatcaccgg aaacatagac gaaatcgcac tgttcccagc cccttttttt
     3121 catatcttct ctgttgatcg gtaaataatc atgtaccatt tataaaacct cgtaaaaatc
     3181 atggatcagc cggtcagcca gctgtttttc tacttcacga gcagctttcg tacgggcttt
//
LOCUS       DBMDUA010000269         3214 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_10403_length_3214_cov_2.142306, whole genome shotgun sequence.
ACCESSION   DBMDUA010000269 DBMDUA010000000
VERSION     DBMDUA010000269.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10403_length_3214_cov_2.142306"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            106..1341
                     /locus_tag="ACZPCL_13155"
     CDS             106..1341
                     /locus_tag="ACZPCL_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR1163449.1"
                     /translation="MDKKKIWESYRFPLILLGGIILGAILGAILGEKATVLAPIGNVF
                     INLLFMVVVPMVFVSIVNAVGSMLNMNRLGKILGSLIGVFIVTGLIASVLVLVVVNVF
                     PPAANTTIEMTSGEMGEMASVGDMIVSTLTVDDFSGLMSRKNMLPIIVAAIFTGLAVA
                     KCGGQESPFGKVMCNLNDIIMKIVDMIMKFAPLGLGAYFANLVGEFGPQLIGDYGRCM
                     LIYYPMCIVYGVVFFPLYAYFAGGKKGVKAMAKNIFNPAITAFATQSSCATIPVNQEA
                     CDKMGVPQDISNIVLPMGATMHMDGSVLSAITKIAFLYGVFNMQFTGVGTYAMAIAVA
                     VLSAFVLSGAPGGGLVGEMLIVSLFGFPGEAFPLIATIGFLVDPMATCLNASGDAVAS
                     MMITRLVEGKDWINKKING"
     gene            1446..1697
                     /locus_tag="ACZPCL_13160"
     CDS             1446..1697
                     /locus_tag="ACZPCL_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HIR1163450.1"
                     /translation="MKVTVIGSHLCPDTLYALQQLAAAGAEIDFKDILSCHADLKAYL
                     NLRDNSDLYAQIRGTERLGIPCFVKEDGTTTLNLAEVLK"
     gene            1870..3147
                     /locus_tag="ACZPCL_13165"
     CDS             1870..3147
                     /locus_tag="ACZPCL_13165"
                     /EC_number="4.4.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0080146 - L-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cysteine desulfidase family protein"
                     /protein_id="HIR1163451.1"
                     /translation="MHALTELIKSDMKPALGVTEPGAIAFAVSTAKAHIKGTVKRVGL
                     RLNSGMYKNAFTCGIPGTSEVGNYYAAALGAVAADPKKGLECLEGIGKAEEEAAGEMI
                     KAGMISVSMSSITSRIFIEATVTGETSEAVVTIQDAHTNITKITENGTVVFEKEAKAA
                     QESKEATPLIHQYTLAQMVEYVNTVSAQELDFIKEAYKVNYELLQEGLKSPKTTYGPY
                     LLKKNGGVLVSEDERKTASLFCNGAIEARVIGLSKPAMSITGSGAHGIIATMPLYAVY
                     KINNYTDEQLLRATALSYLVCMYIKEYSGKLSAFCGCAIAAGTGMACALAWLKGAGAE
                     TMEKVINNMASSITGMICDGGNQGCTMKGIVAVDAAFDSVELAMDDVYIYNAHGINGA
                     TPEETMHNMGLIASPGMTGTEKTIVDILDGKNK"
BASE COUNT          973 a          588 c          778 g          875 t
ORIGIN      
        1 gctgtagatg cactggataa ataaaataca gtacaattta aaacagtgca atttaaaaca
       61 atataattta caacaataca aaacgagata attttggggg aaattatgga caagaagaaa
      121 atctgggaaa gctaccgctt cccgctgatc cttttaggag gaattatttt aggagcgatc
      181 cttggagcga ttttaggaga gaaagcaacg gtacttgcac caattggcaa tgtattcatc
      241 aacctgctgt ttatggtcgt agtacctatg gtttttgttt ctattgttaa tgcagtagga
      301 agtatgttaa atatgaaccg tcttggaaag atccttggct ccctgatcgg tgtatttatt
      361 gtaaccggcc tgatcgcatc tgttctggta ctggtagttg taaatgtatt cccaccagct
      421 gcaaacacca ctattgagat gaccagcggt gaaatgggag aaatggcaag tgttggtgat
      481 atgatcgtta gtacactgac tgtagatgat ttctccggtc tgatgagcag aaagaatatg
      541 cttccgatca ttgttgcagc tatctttacc ggtcttgcag tagcaaagtg cggcggacag
      601 gaaagcccat ttggtaaagt aatgtgcaac ttaaatgaca tcatcatgaa gatcgttgat
      661 atgatcatga aatttgctcc tcttggtctt ggtgcatatt ttgcaaacct ggtaggtgaa
      721 tttggtcctc agctgatcgg tgattatggc cgctgcatgc tgatctacta tccaatgtgt
      781 attgtttacg gcgttgtatt cttcccgtta tatgcttact ttgcaggcgg aaagaaaggc
      841 gtaaaagcca tggcaaagaa catcttcaac ccggctatta ctgcatttgc aacacagagt
      901 tcatgtgcaa ccatccctgt aaaccaggaa gcatgtgata agatgggtgt acctcaggat
      961 atcagtaata tcgttcttcc tatgggtgct accatgcata tggatggttc tgtattatcc
     1021 gctattacaa agatcgcttt cctgtacggt gtattcaaca tgcagtttac aggtgttgga
     1081 acctatgcaa tggcaatcgc agtagcagtt ttaagtgcat ttgtattatc cggagctcca
     1141 ggcggcggcc ttgtaggtga aatgcttatt gtcagcctgt tcggtttccc aggagaagca
     1201 ttcccactga tcgcaaccat cggtttctta gtagacccaa tggctacctg cttaaatgca
     1261 tctggtgatg ctgttgcttc tatgatgatc acacgtcttg tagaaggcaa ggactggatc
     1321 aacaaaaaaa tcaatgggtg atccatttaa tggataagct gcctgccagt gcaggcagtg
     1381 gagcgcgttg gcacggattt atgcatacaa tacaaaaata cataaaagac gaaggagaaa
     1441 tcactatgaa agttacagta atcggcagtc atctttgccc agacactctt tatgctttac
     1501 agcagcttgc tgcagcaggg gctgaaattg attttaaaga cattttatcc tgccatgcag
     1561 atcttaaagc atacttaaat ctgcgtgata actctgatct gtatgcacag atccgcggta
     1621 cagaacgtct tggaattcca tgcttcgtaa aagaagacgg aactactact ttaaatctgg
     1681 cagaggtttt aaagtaatta aagagatctt tatttaggat cgttcattag gatacgatat
     1741 aaaaaaatgt aaatgatcgc gtaagcgatg tcagagtgag taaaggggga tgctgtgaaa
     1801 tgataggaac attttgcagc atcctttttg caaagtcttt gggatcatag aattgaggag
     1861 aaaaataaga tgcatgcatt aacagaactg ataaaaagtg atatgaaacc agctcttggt
     1921 gttacagaac cgggagccat tgcttttgct gtatccacag caaaagccca tataaaaggc
     1981 actgtaaaaa gggtgggttt aagattaaac agcggtatgt ataaaaatgc ctttacctgc
     2041 ggtattccgg gaacaagtga ggtgggaaat tattacgctg ctgctttagg cgctgttgct
     2101 gcagatccaa agaagggtct ggaatgtctg gaaggtattg gaaaggcaga ggaagaagct
     2161 gcaggcgaaa tgatcaaagc gggaatgata tcagtttcca tgagcagtat taccagccgc
     2221 attttcattg aagcaactgt aacaggtgag acttcagagg cagttgttac tattcaggat
     2281 gcccacacta atattacaaa gatcacagaa aatggaactg tagtatttga aaaagaagca
     2341 aaagcagcac aggagagtaa agaggctaca ccgctgatcc atcagtatac cctggcgcag
     2401 atggtagagt atgtaaatac tgtttctgca caggaactgg attttatcaa agaagcatat
     2461 aaagtaaact acgagctgct tcaggaaggc ttaaagagtc caaagactac ctacggacca
     2521 tatctgttaa agaagaatgg tggggttctg gtatcagaag atgaaagaaa gactgcttca
     2581 ttattctgca acggagccat tgaggcgaga gtcatcggtc tttccaaacc ggctatgagc
     2641 atcaccggtt ccggtgccca tggtattatt gcaactatgc cgttatatgc tgtatacaag
     2701 atcaataact atacagatga gcagcttctt cgtgctactg cattaagcta tctggtatgt
     2761 atgtacatta aggaatactc cggcaagctg tctgccttct gcggctgtgc tattgcagca
     2821 ggaacgggaa tggcctgtgc actggcatgg ttaaaaggcg ccggcgcaga aacgatggaa
     2881 aaagtcatca acaacatggc aagcagtatt acaggtatga tctgtgatgg cggcaaccag
     2941 ggatgtacca tgaaaggtat tgtggcagta gacgcagctt ttgactctgt agaactggct
     3001 atggacgatg tatatattta caacgcccat ggtatcaacg gtgcaactcc ggaagagacc
     3061 atgcacaata tgggactgat tgcgtcacct ggtatgacag gaacagagaa gaccattgtg
     3121 gatattcttg acggaaagaa taagtaatat tgtgcaaaac ctttggaagg aggaattcta
     3181 tggagttttg gaaaatgaat ggtgctggaa atga
//
LOCUS       DBMDUA010000270         3150 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_10665_length_3150_cov_2.699246, whole genome shotgun sequence.
ACCESSION   DBMDUA010000270 DBMDUA010000000
VERSION     DBMDUA010000270.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10665_length_3150_cov_2.699246"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="ACZPCL_13170"
     CDS             <1..597
                     /locus_tag="ACZPCL_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter permease"
                     /protein_id="HIR1163452.1"
                     /translation="YYIPEIASQFFVMGLVAGVIGLIFRLNDMHLNDIPAAFNQGAAD
                     LLGAAMCVGMAQGIIIILGGTDASSGTVLNTILHSLSGAMQNFPPVVSAWLMYVFQSV
                     FNFFVVSGSGQAALTMPIMAPLADLVGVERQVAVLAFQLGDAFTNFIVPTSGCLLGAL
                     AAAKLEWGSWAKFQIKFQAVLVVFASLAVVLGVVIGLS"
     gene            724..1044
                     /locus_tag="ACZPCL_13175"
     CDS             724..1044
                     /locus_tag="ACZPCL_13175"
                     /inference="COORDINATES: protein motif:HMM:NF017920.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HIR1163453.1"
                     /translation="MELMIIIVQEQDYPKLSSTFIHNRVMATKFSTEGLYLNKKNITL
                     MVCIEKEREEEILDYIRQSCTERMEERQVCEYNGMTMVDVTKGVKVGGAIVFFLDVDR
                     MMKY"
     gene            1195..1863
                     /locus_tag="ACZPCL_13180"
     CDS             1195..1863
                     /locus_tag="ACZPCL_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1163454.1"
                     /translation="MEEALSCIGKMPIFSGIREEELYTLIKCCDGQMAVFEKDQWIFQ
                     AGEELGYIMVVLKGRLAIIQEDFWHQQKETIHIMPGEIFGENYASAGSKILPVGIKAA
                     ENSEVLFLDYERSLTFCTMACPFHSMMVHNLIRIMSANKGRAEEKLEHISQKTTRDKL
                     RSYLSRKALQEGSYSFDIPYNRQELADYLGVDRSAMSSELGKMKAEGILEFKKNHFIL
                     NKLK"
     gene            complement(1981..>3150)
                     /locus_tag="ACZPCL_13185"
     CDS             complement(1981..>3150)
                     /locus_tag="ACZPCL_13185"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR1163455.1"
                     /translation="NGCCGFRGFECCCIDCGQGKELEGLEERAKLSGASKLYIEDLVD
                     DFCDNYIMPCVKANAVYENEYLLGTSMARPAIAKRLVDIARKEGAVAICHGATGKGND
                     QIRFELSIKALAPDIKIIAPWRMTDVWTMQSREDEIEYCKQHGIDLPFDHSHSYSRDS
                     NIWHVSHEGLELEDPSLEPNFSNMLVMGVTPEQAPDEDEYVTMTFEKGVPTSVNGVKM
                     KVSDIIRKLNELGGKHGIGIVDIVENRVVGMKSRGVYETPGGTILMAAHRQLEELVLD
                     RATMEVKKDMGNKLAQIVYEGKWFTPLCDAVKAFVESTQEYVTGEVKFKLYKGNIIKA
                     GTTSPYSLYSESLASFTTGDLYDHHDADGFITLFGLPLKVRAMKLAELKKENNQK"
BASE COUNT          874 a          670 c          730 g          876 t
ORIGIN      
        1 tactatattc ctgagattgc ttcccagttc ttcgtaatgg gtctggttgc cggtgtgatc
       61 ggactgatct tccgtctgaa tgatatgcac ttaaatgata tcccggcagc attcaatcag
      121 ggtgcagctg atctgttagg tgcagcaatg tgcgtaggta tggcacaggg tatcatcatt
      181 atcttaggtg gaaccgatgc ttcctccgga actgtattaa acaccattct ccacagcctt
      241 agcggtgcaa tgcagaactt cccgccagta gtttccgcat ggcttatgta cgtattccag
      301 tccgtattca acttcttcgt tgtatccggt tccggacagg cagcacttac catgccgatc
      361 atggcacctt tggcggatct ggtaggcgta gagcgtcagg tagctgttct tgcattccag
      421 cttggtgatg catttacaaa ctttatcgta cctacttccg gctgtctgtt aggcgcattg
      481 gctgcagcaa aactggagtg ggggagttgg gcaaagttcc agatcaagtt ccaggcagtc
      541 ctggttgtat ttgcttccct ggcagtggtc cttggcgttg tcatcggact gtcataatgt
      601 tacagttcaa ctgtaacgtc ataattgcca taacaaaagc ttaacttgct tatccatttt
      661 actgagatta taatattcct taaacgtccg caaaaggcgg aaacgtgaaa ggagtgcagc
      721 aaaatggagt tgatgatcat tattgttcag gaacaggatt atccaaagct ttcaagtact
      781 tttatccaca atcgtgtgat ggcaacgaag tttagtacag aaggactgta tctgaataag
      841 aaaaatatca cattgatggt atgcattgaa aaagagcggg aagaggagat cctggattat
      901 atccgccaga gctgtacaga acgaatggaa gaacgtcagg tttgcgaata taatggaatg
      961 actatggtag atgttaccaa aggcgtaaag gtaggcggcg ccattgtatt tttcctggat
     1021 gttgaccgga tgatgaagta ttaaattata aatagtgaaa gagagatcca ccttgcgagg
     1081 ctgttgcagg gtggattttt ttggcaccgc ttgtttttct cctgcactca ttatataatg
     1141 ttaccaaaag gttgtaaaaa caacgaaaag cgaaagactg cgaggaaaag acacatggaa
     1201 gaagcattaa gttgtattgg gaagatgcct atatttagtg ggatcaggga agaagagctg
     1261 tatacactga tcaaatgctg tgacggacag atggcagtat ttgaaaaaga ccagtggatc
     1321 tttcaggcag gagaagaact gggatatatc atggttgttt taaagggacg cctggcgatc
     1381 attcaggaag atttctggca tcagcaaaaa gagacgatcc atattatgcc gggagagata
     1441 ttcggtgaaa attacgccag cgcaggcagt aaaatattgc ctgtaggtat taaagcagca
     1501 gaaaacagcg aagtcctgtt tttagattat gaacgttccc ttactttctg cacgatggca
     1561 tgcccgtttc acagcatgat ggtccataat ctgatcagga tcatgagtgc aaacaaaggc
     1621 cgtgcagaag aaaaattgga acatatatcc cagaagacta ccagggataa actgcgttcc
     1681 tatttatcac gcaaagcttt acaggaaggc agctacagct ttgatatacc ctataaccgt
     1741 caggaactgg ctgactattt aggcgtagac cgcagcgcca tgtccagtga actgggaaaa
     1801 atgaaagcag aagggatcct ggaatttaag aaaaatcatt ttatattaaa taaattaaaa
     1861 taagatcgat gcataaaaat accccggagc gattggatca ttcagatctc gctccggggt
     1921 attgattttc atattcagat caattcagac caactgtatg gcatttgcct gacagtaaaa
     1981 ttacttctga ttattctctt tcttcagctc agctaacttc atagcgcgaa cctttaatgg
     2041 aagtccgaac agagtgatga agccgtctgc atcgtggtgg tcgtaaaggt caccggttgt
     2101 gaaggatgct aaggactcgc tgtataagga gtatggagaa gtggttccgg ctttgatgat
     2161 gttgcccttg taaagtttga acttaacttc accggttaca tattcctggg tggactctac
     2221 gaatgccttt acagcgtcgc aaagaggagt aaaccatttt ccttcgtaaa cgatctgagc
     2281 cagcttattg cccatatcct tcttaacttc catagtagca cggtcaagaa ccagctcttc
     2341 cagctgtctg tgagctgcca ttaagatagt tccgcctgga gtctcataaa cgccacggga
     2401 tttcatacct acaacacggt tctcaacgat atctacaata ccgataccat gctttccgcc
     2461 cagctcattt aacttgcgga taatatcaga aactttcatc ttaacgccgt ttacgctggt
     2521 aggaacacct ttttcaaagg tcatagtaac atattcatcc tcatctggtg cctgttccgg
     2581 ggtgacaccc ataaccagca tgttggagaa gtttggctct aaggaaggat cttccagttc
     2641 cagaccttcg tggcttacat gccagatgtt gctgtcacgg ctgtagctgt ggctgtggtc
     2701 aaatggaagg tcaatgccat gctgcttgca gtattcgatc tcatcctcac gggactgcat
     2761 ggtccatacg tcagtcatac gccatggagc gatgatcttg atgtccggag ccagtgcctt
     2821 gatagacagc tcaaaacgga tctggtcatt tcccttaccg gtagcaccgt ggcagatggc
     2881 aacagcgcct tctttacgtg cgatatcaac cagtctcttt gcgattgcag gacgagccat
     2941 ggaagtacct aataagtatt cattttcgta aactgcattt gcctttacac atggcataat
     3001 gtagttgtcg cagaaatcat ctactaaatc ttcaatatat aatttagaag cgccggacag
     3061 ctttgctctt tcttccagtc cctctaattc ttttccctgt ccacagtcga tacagcagca
     3121 ttcaaatcca cggaatccgc agcatccatt
//
LOCUS       DBMDUA010000271         3121 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_10797_length_3121_cov_2.077483, whole genome shotgun sequence.
ACCESSION   DBMDUA010000271 DBMDUA010000000
VERSION     DBMDUA010000271.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10797_length_3121_cov_2.077483"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(823..954)
                     /locus_tag="ACZPCL_13190"
                     /pseudo
     CDS             complement(823..954)
                     /locus_tag="ACZPCL_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008750663.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(951..>3121)
                     /locus_tag="ACZPCL_13195"
     CDS             complement(951..>3121)
                     /locus_tag="ACZPCL_13195"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1163456.1"
                     /translation="IMIQEEVDLEEYKMEPLKAVFRFDADEKGTLYMEPLLSYGEYTF
                     HPIEDENLPSAICRDVPGEFKISQVIRKYFKCRDPKDGRLVLKEDEKALYHLLDQGME
                     EFRGLGDVYLSESMKNWKIVETPSVSAGVSAYSGWLELTVDMGEFPKEELGRILTAYS
                     QKKKYYRLKSGQFLMLDQGGMFTLTKLAGELGISKKDLQSGTIRLPAYRALYLDHILK
                     EGPGITYYRDQLFKSMVRAVKTVEDSDEPVPARFEAILREYQKTGFRWMKTLDKCGFG
                     GILADDMGLGKTIQVIALFEDTYSSGEKAPSLVVCPASLVYNWEQEIQKFAPDLKVRS
                     VVGTGPQRLELLEKIKEDPSGCQILITSYDLLKRDIAHYEGIDFRFQIIDEAQYIKNA
                     TTQSARSVKAVQVRSRFALTGTPIENRLGELWSIFDFLMPGFLFGNQYFRKEYEVPIT
                     KDRDEQVLKRLKGLIGPFILRRVKKDVLKELPDKLEKVVYSTLEEEQKKLYTANALLL
                     KEKLEADDFKGAEKLQILSDLMQLRQICCDPRLCYGNYKAGSAKLKTCMDLIRTGVEG
                     EHKILLFSQFTSMLDLIEEELKKEGIECYKLTGATPKEERIQMVSRFHQNEVPVFLIS
                     LKAGGTGLNLTAADIVIHYDPWWNVAAQNQATDRTHRIGQEKQVMVYKLITSDTIEEN
                     ILNLQEAKRNLADQIVTGGGISFGELTKADILKMIEESRP"
BASE COUNT          820 a          760 c          611 g          930 t
ORIGIN      
        1 gaagcgctcc tgaaaacaaa cagttctatt ttttatattt cgatacaatt tgttggcatg
       61 caatacgaca taacctacaa aaatccgtgc caccacctgc aatcggcagt tcatacgtta
      121 aactcaggtt tatatttgac acaattgacg gaatgttcta tactgaatgc acatcaaagt
      181 cgcagacata tgcggttgtg attgtgaagc tttttcatag ttgcaaacat tcttatttcg
      241 tttctgtctg cagattccaa accacgtttt gtggtgtaga atataatctc agatttcaca
      301 gccgtttcgg cacgagattc cattttgatg aaaaattcaa tgcatatgaa ttgtaacatg
      361 gattattaca gcatcctgtc ctgtattttc gataggaaaa atagcttgta cgcattgaaa
      421 aataccacac attctattat cataaagggg gaacgtgatt gaacacaaac gatgagaaaa
      481 tccaatggca tcctgcattt gacgcggcac tgcagattga gtttggagaa gaggcagaat
      541 atctggaatt cgattccgaa catctgcttt ccaaaaagcc aatgcagatt gatgtgttga
      601 tcaaaaatga aaaacatgta aagatccaga agaatattgg caggattttc cggcagtaca
      661 atatcataga atacaaatca ccggaagaca atcttgacat tgatgacttt tataaggtgt
      721 acgcatatgc ctgtatttac aaggctgata cggagaaaat tgatcttatc ccggcagcag
      781 agctgacaat cacttttgta tgctatcatt atccgagagc catgccaggg aaactgctac
      841 attggcttct gcgcctccgt aacttgcctc aaatgcatct gtctgcaaaa tcctcatatg
      901 ctccggagtt tttaatcgga gcatgatctc accaaaagtt actgttcttt tcatggtctg
      961 ctctcctcta tcatctttaa aatatctgct tttgtaagtt cgccaaagga aatgcctcct
     1021 ccggttacga tctggtctgc cagatttcgc tttgcttcct ggagatttaa aatattttct
     1081 tcaatggtat cactggtgat cagcttatat accataacct gcttttcctg accgatcctg
     1141 tgggtacggt ctgtcgcctg gttctgggct gccacattcc accatggatc atagtgaatc
     1201 acaatatctg ccgctgtcag gttcagtccc gttccccccg cctttaaact gatcagaaat
     1261 acgggaacct cattctggtg gaagcgggat accatctgga tccgttcttc ctttggcgtt
     1321 gcccctgtca gcttataaca ttcgatcccc tcttttttca gttcttcctc gatcagatcc
     1381 aacatagagg taaactggga gaataataag atcttgtgtt ctccttctac acctgtacgg
     1441 atcagatcca tacaggtctt aagcttggca gagcctgcct tgtaatttcc atagcagagc
     1501 ctcggatcac agcagatctg gcgcagctgc ataagatctg ataagatctg cagcttttct
     1561 gctcccttaa agtcatctgc ttccagcttt tcttttaata aaagggcatt tgccgtataa
     1621 agcttcttct gttcttcctc taaagtagaa taaactactt tttccagctt atccggaagt
     1681 tcttttaaca catctttctt tactcttcgc aggataaacg gtccgatcag tccctttaag
     1741 cgttttaaca cctgctcatc ccggtctttc gtaataggta cctcatattc cttgcggaaa
     1801 tactgatttc caaacaaaaa tccgggcata aggaaatcaa aaatactcca taactctccc
     1861 agcctgtttt caataggcgt accggtcagc gcaaaacggg aacgcacctg gacagccttt
     1921 acagagcggg cactctgggt agtggcattt tttatgtact gggcttcatc aatgatctgg
     1981 aatcggaaat caatcccctc atagtgggca atatccctct ttaacaggtc ataggaagtg
     2041 atcaggatct ggcagccgga aggatcttct tttatttttt ccagcagttc caatctctgg
     2101 ggtcctgttc ccactacaga ccgaaccttc agatccgggg caaatttctg gatttcctgc
     2161 tcccagttat aaaccaggga tgctgggcag actacaaggc ttggggcctt ctccccgctg
     2221 ctgtaggtat cttcaaaaag agcgatcacc tgaatggtct ttccaagacc catatcatcc
     2281 gctaaaatgc cgccgaagcc gcatttatcc agggttttca tccaccggaa gccagttttc
     2341 tggtattccc ttaaaatagc ttcaaaccgg gcaggaaccg gctcatcgct gtcctcaacc
     2401 gtctttacag cacggaccat tgacttaaac agctggtccc tgtaataggt aatgcccgga
     2461 ccttctttta aaatatggtc cagatacagt gctctataag caggcaggcg gatggtgccg
     2521 ctttgtaagt cctttttgct gattcccagc tctcccgcca gttttgtaag ggtaaacata
     2581 ccgccctggt ccagcattaa aaactgtccg ctctttaagc ggtaatattt ctttttctgg
     2641 ctgtaggcag tcaggatacg tcccagttct tcttttggaa attctcccat atccacggtc
     2701 agttccagcc agccggaata agcgctgaca cctgcagata cagaaggagt ttccacaatt
     2761 ttccagtttt tcatggactc agacaggtat acatctccca gtccccggaa ttcttccatt
     2821 ccctgatcta acaagtggta caaggctttt tcatcctctt ttaataccag cctgccgtct
     2881 tttggatccc tgcacttaaa atacttgcgg atcacctggc tgatcttaaa ctctcccggc
     2941 acatcacggc agatcgcaga cggaagattt tcatcttcta tgggatggaa ggtatattcc
     3001 ccataagata aaagaggttc catatataaa gtgccttttt cgtctgcgtc aaaacggaac
     3061 accgctttca aaggctccat tttatattct tccagatcta cttcttcctg gatcatgatg
     3121 c
//
LOCUS       DBMDUA010000272         3050 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_11098_length_3050_cov_2.891489, whole genome shotgun sequence.
ACCESSION   DBMDUA010000272 DBMDUA010000000
VERSION     DBMDUA010000272.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11098_length_3050_cov_2.891489"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="ACZPCL_13200"
     CDS             complement(<1..901)
                     /locus_tag="ACZPCL_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HIR1163457.1"
                     /translation="MSKWYEKTLEEKSNIIYSRIRLARNWDEYVFPSRLDEEGSRELV
                     DGLKDGLKEIPYPYYALENMQQAEKKALRERRILNRCAVEKTYAQGLYLSEDESESIL
                     LGADDHIRMQFLAPGLCLEKLWQKADAADDRINEHFPYAYDEKYGYLTTFPTNMGTGL
                     KACVVIHLPMLSQLKKFQGIVADMGRLGTSIRGLFGEGSENAGNMYELSNQRTLGLSE
                     REIIELVNKAAVQLNSQEQKVRKAALNARKLEREDETYKSYGVLKYARKMAEKDARVF
                     IGQLISGETDGLISFEKEESLYSL"
     gene            complement(894..1502)
                     /locus_tag="ACZPCL_13205"
     CDS             complement(894..1502)
                     /locus_tag="ACZPCL_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HIR1163458.1"
                     /translation="MLCEKCKVREANIKYTEIINGVKTEHNLCSHCAKEMDFGQYTAL
                     LDGEFPLGKLLSGLLGLEEDEEETDERSRVVCPTCGTSFDDFVQNSRFGCPDCYGVFD
                     LFINDKIKQLQGSESHRGKQPKYQRAQETISENNEEMVPADVLQKAGENAAKEQPEGM
                     KEITQLERRLKEALEKEDYETAAACRDQIRALKAGKGGEVHE"
     gene            complement(1504..1893)
                     /locus_tag="ACZPCL_13210"
     CDS             complement(1504..1893)
                     /locus_tag="ACZPCL_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1163459.1"
                     /translation="MILKIDFNSDEAIYIQLRNQIIMGIATDMIREGDTLPSVRQMAD
                     HIGINMHTVNKAYSVLRQEGFVKLDRRRGAVVSLDVDKIQAIDEMRQNLDVVLACGIC
                     RDVSREEAHQLVDEIYDRYTKREARED"
     gene            complement(2012..2791)
                     /gene="udp"
                     /locus_tag="ACZPCL_13215"
     CDS             complement(2012..2791)
                     /gene="udp"
                     /locus_tag="ACZPCL_13215"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HIR1163460.1"
                     /translation="MPVAYSVGNEEYHLKIRPGDVGRYVILPGDPGRCEKIARYLEDP
                     RFVASNREYTIYTGTLEGEKVSVCSTGIGGPSAAIAMEELIHCGAECFIRVGTCGGIE
                     ENVLGGDLVIATGAIRMEGTGLQYAPVEFPAVADFQVVNALAEAAKADGQRFHTGVVH
                     CKDSFYGQLDPDSMPVRGELKSKWQAWLDCGALASEMESAALFVVAAARRVRVGTVLL
                     VLGNQTRRAKGLEDIQCHDTERAVRVAADAMRRLIKRDKTS"
     gene            complement(2857..>3050)
                     /locus_tag="ACZPCL_13220"
     CDS             complement(2857..>3050)
                     /locus_tag="ACZPCL_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163461.1"
                     /translation="SLRRRVLSRAASVVTCEGFRPDTWSGFDAESMSDCPVYDFHEID
                     AIMEIVLKEAEKFWDNRRI"
BASE COUNT          693 a          877 c          557 g          923 t
ORIGIN      
        1 aaagactgta cagactttct tctttctcaa aagagatcag tccgtctgtc tctccggaga
       61 tcagctgtcc aataaatacc ctggcatctt tttctgccat tttccgcgca tactttaaaa
      121 caccatagga tttatacgtc tcgtcttccc gctccagctt tctggcattt aaagcggctt
      181 ttctcacttt ctgttcctga ctgtttaact ggacagctgc tttgtttacc agctcaatga
      241 tctcccgttc tgacagtcct aaggttctct ggtttgacag ttcgtacata ttgcctgcat
      301 tttcactgcc ttccccgaaa agtccccgta tggacgttcc cagtcttccc atatctgcca
      361 cgatcccctg aaatttcttc agctgggaca gcataggcag gtggatcacc acgcaggcct
      421 taagtcctgt tcccatattg gtagggaacg tggtcagata accatatttt tcatcatagg
      481 cataagggaa atgttcattg atcctgtcgt ctgcagcatc tgctttctgc cacagctttt
      541 caaggcacag accaggtgcc aaaaactgca tccttatatg gtcatctgct cccagtaaaa
      601 tactttctga ctcgtcttct gacaggtaca gtccctgtgc gtatgttttt tccactgcac
      661 agcggtttaa gatccgccgt tcccttaagg ctttcttttc tgcctgctgc atattttcca
      721 gagcatagta gggataaggg atctctttta aaccgtcttt taagccgtct accagctccc
      781 tgcttccttc ttcgtccagt ctggaaggaa atacatattc atcccagttt cttgcaaggc
      841 ggatcctgct gtatataata ttggactttt cttccaatgt tttctcatac catttactca
      901 tgcacttcgc cgccctttcc tgcctttaat gcccggatct gatcccggca tgcagctgct
      961 gtttcataat cttctttttc cagggcttct tttaatcttc gctccagctg cgttatctct
     1021 ttcatgcctt ccggctgctc ttttgcggca ttctctcctg ctttctgcaa aacatcagcc
     1081 ggtaccatct cttcattgtt ttcagaaatg gtttcctgtg ccctctgata ttttggctgt
     1141 tttcccctgt ggctttcact gccctggagc tgcttgatct tatcattgat aaaaagatca
     1201 aaaacgccat agcagtccgg acagccgaag cggctgtttt gaacaaaatc atcaaaactg
     1261 gtaccgcagg taggacacac caccctggaa cgttcatcgg tttcttcttc atcttcctcc
     1321 aaccctaaaa gtccggaaag cagttttccc agcgggaatt ctccgtctaa caaagccgta
     1381 tactgtccaa aatccatttc cttcgcacag tgggagcaga gattatgctc cgtttttact
     1441 ccgttaatga tctctgtata tttaatattc gcttcccgta ctttgcattt ttcacataac
     1501 atattaatcc tcccgggcct cacgcttcgt atatctgtca tagatctcat ctaccagctg
     1561 atgcgcttcc tccctggaaa catccctgca gatcccgcag gcaagcacta catccagatt
     1621 ttgacgcatc tcatcgatgg cctggatctt atccacatcc agggaaacta cagcccctct
     1681 gcggcggtcc agtttcacaa acccttcctg gcgcagaaca gaatacgctt tatttactgt
     1741 atgcatatta atgccgatat gatctgccat ctgtcgcaca gaaggtaagg tatccccttc
     1801 ccggatcata tctgtagcaa tccccatgat gatctggttg cgcagctgga tatagattgc
     1861 ttcatcgctg ttaaaatcaa ttttcagtat cataaaccca ccaactttca ggggctgccg
     1921 ctacaaaatc tttgtctgcg gcagccccac tgtttttatc tgctaagtta tatctgttct
     1981 aataataata taacaacttt ttttgcatcc gtcaagaagt tttatctctt ttgatcaggc
     2041 gcctcatggc atctgctgct acacgcactg cccgctccgt atcatggcat tggatatcct
     2101 caagtccttt agcccgcctt gtctggtttc ccagtactaa aaggaccgtt cccacgcgga
     2161 ctctccttgc tgctgccaca acaaacagag cagctgactc catctcactg gcaagagcgc
     2221 cacagtcaag ccacgcctgc cattttgact ttaattcccc tcttacgggc atactgtccg
     2281 gatctaactg gccataaaaa gagtctttgc agtggacaac tcctgtatgg aacctctggc
     2341 catctgcttt tgctgcctct gccagggcat ttacaacctg aaaatccgcc actgccggaa
     2401 attcaacagg cgcgtactga agcccggtgc cttccatacg gatcgcaccg gtagcgatca
     2461 ccagatcacc gcccagtaca ttttcctcta taccaccgca ggttcccaca cggataaagc
     2521 actcagcgcc gcagtggatc agttcttcca ttgcgatggc agcagacggg cccccgatgc
     2581 ctgtactgca cacacttact ttctctcctt ccaatgtacc tgtataaatg gtatattccc
     2641 tgtttgaagc aacaaatctg ggatcttcta aatatctggc aatcttctca catctgcccg
     2701 gatcacctgg caggatcaca taccgtccta catctcccgg acgtattttt aaatgatatt
     2761 cctcgtttcc tacactgtat gcaactggca taataatccc tcctgttgta tccataaatt
     2821 ttctgttttt attgtaccac acccctgtat cgtctgtcaa attctccggt tgtcccagaa
     2881 cttttctgct tcttttaaaa cgatttccat aatagcgtcg atctcatgaa aatcatacac
     2941 cggacagtct gacatagact ctgcatcaaa tccggaccat gtatccggcc tgaaaccttc
     3001 acaagtgacc acactggctg ccctcgacag cacccgcctg cgcagtgaac
//
LOCUS       DBMDUA010000273         3049 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_11106_length_3049_cov_2.933853, whole genome shotgun sequence.
ACCESSION   DBMDUA010000273 DBMDUA010000000
VERSION     DBMDUA010000273.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11106_length_3049_cov_2.933853"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(181..2127)
                     /locus_tag="ACZPCL_13225"
     CDS             complement(181..2127)
                     /locus_tag="ACZPCL_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR1163462.1"
                     /translation="MTHKFPHLCSPITIAGRTFSNRMFSAPMGGTDITNDGCIGPKST
                     AFYELRAKGGAAAVTVSECMVHPETDGSHAYHLDTTILNSLAAATYTADAISRHGAAA
                     SLELSHSGMYAGTYMTDKSRQHEMCQWGASDTVRADGVKVKSLSKEQIKDIVASYGKV
                     AGLAKRAGFEMILIHGGHGWLINQFLSPYFNHRTDEYGGSLENRCRLASEILDAVREA
                     VGPAFPIEFRLSGSELFEGGYDLEEGVRIAMQLESKIDLLHVSAGTYQRGFGDTHPSM
                     FKEHGCNVYLAAEIKKHVSIPVATLGGLNDPAQMEEIIASGKADVVYMARALLADPFL
                     PRKVMENRDEDIVRCLRCFTCMAERAATSTRRCTVNPLIGREMEGDEVQPAPVKKKVL
                     VAGGGPGGLYAAWTAARRSHQVILCEKEEELGGILKSEIAIPFKKEMYQLTETYARFA
                     RQSGVEIRLGCEVTPEYVEKEAPDALIIAVGSRPLVPPIPGIKGENVIIVNELYKNQD
                     RVADKVAVMGGGLAGCECAIHLGMEGKEVHLIEMRDELAVDANVRHRPLLLKEIEKYV
                     HVHTGCRVEEVTADGVRCRDKDGNELMVGAQTVICALGQRGRTDVVEALRNGAPFVRV
                     IGDARKVSTITNAVYQGYHAALDI"
     gene            complement(2265..2966)
                     /locus_tag="ACZPCL_13230"
     CDS             complement(2265..2966)
                     /locus_tag="ACZPCL_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczE/YyaS/YitT family protein"
                     /protein_id="HIR1163463.1"
                     /translation="MKRTKAEQVKRYLIFLVGLFVNSLGVSLITKGNLGTSPISSIPY
                     VLSLNFPFSLGNFTIVFSLLLIFLQFLLLGKNFKAEHLLQIPVSMAFGYFIDMSMILL
                     GFVHPVSYISKVLGLLIGCLVLGVGVYMEVLADVVMLPGESFVRAVVFRIHSEFGFTK
                     VCFDVSMAVIAAVLSFLLSGHLEGVREGTIVAAVLVGFIARSIGKRLSFLPVLIYGDD
                     KKEEEKIEEELNDIV"
BASE COUNT          810 a          773 c          617 g          849 t
ORIGIN      
        1 actttttggg caaaatgtca atatcacagt taccatttcc ctatgcgctg gtttgaaccg
       61 taacaatcaa aaaatactcc cagccacagg tatgttctcc tgtaatgact gagagtattt
      121 ttaccaaaac tataaaactt tatgcaatat gatcatagga tcgaacttta taaattgatt
      181 ttaaatatcc agtgctgcat gatacccctg atatacagca tttgtgatag tagaaacttt
      241 tctcgcatct ccgatcacac ggacaaatgg cgcaccgttt ctgagagctt ctacaacatc
      301 cgttctgcct ctctgtccaa gggcacagat cacagtctgg gctcccacca tcagctcatt
      361 gccatcttta tccctgcagc ggacaccatc tgcagttact tcttctacgc gacatcctgt
      421 atggacatga acatatttct caatttcttt taacagcagc ggacggtgac gcacattggc
      481 atctacagcc agttcatccc gcatttcgat caggtgtact tcttttcctt ccatacctaa
      541 atggatggca cattcacagc cggccaggcc gccgcccata acagccactt tatcagctac
      601 tctgtcctgg tttttataca gctcattgac gatgatcaca ttttcgccct taattccagg
      661 gatcggcgga accagtggtc tggagccaac agcgatgatc agggcatccg gtgcctcttt
      721 ttccacatat tcaggtgtca cttcgcatcc taaacggatc tctacaccgc tctgtcttgc
      781 aaaacgtgca taggtttctg tcagctggta catttctttc ttaaatggaa tggcgatctc
      841 actttttaag ataccgccca attcctcttc tttttcacat aagataacct gatggcttct
      901 tctggctgct gtccatgctg catacagacc gcccgggccg cctcctgcta ctaaaacttt
      961 cttctttaca ggtgccggct gtacttcgtc cccttccatt tctcttccga tcaatgggtt
     1021 tacggtacat cttctggtgg aagtagctgc acgttctgcc atacaggtaa aacagcgcag
     1081 gcagcggaca atgtcctcat ccctgttttc catgactttt cttggaagga atggatctgc
     1141 caaaagggcg cgggccatat aaactacatc tgccttgccg gaagcaatga tctcttccat
     1201 ctgtgccggg tcatttaagc cgcccagtgt tgcaaccgga atagaaacat gttttttgat
     1261 ctctgctgcc agatatacat tacatccgtg ttccttaaac atagacggat gggtatctcc
     1321 aaaacctctc tggtaagtgc ctgcagatac atggagcagg tcgatcttgg actccagctg
     1381 catagcgatg cgcacgcctt cttccaggtc atagccgcct tcaaacagct cagagccgct
     1441 taaacggaac tcaataggga atgcaggtcc tactgcttca cggactgcgt ctaagatctc
     1501 acttgccaga cggcagcggt tttccaggga gccgccatat tcatcggttc tgtggttaaa
     1561 atacggggat aaaaactggt tgatcagcca gccatgtccg ccgtggatca ggatcatctc
     1621 aaaacctgca cgttttgcaa gacctgctac ttttccatag gaagctacaa tgtccttgat
     1681 ctgctctttg cttaaagact taacctttac gccatctgca cgtaccgtat cagaagcgcc
     1741 ccactggcac atttcatgct gtctggactt atccgtcata taggttcctg catacatacc
     1801 ggaatgggac agttccaggc tggctgctgc tccgtgacgg gaaatagcat ctgctgtata
     1861 ggtagctgct gccagggaat ttaaaatagt ggtatccaga tgatatgcat gggaaccgtc
     1921 tgtctccgga tggaccatac attcgctgac cgttacagct gctgcgccgc ccttggcacg
     1981 aagctcataa aaggcggtag attttggtcc gatacaaccg tcattggtaa tatctgttcc
     2041 gcccattggt gcggaaaaca tacggttgga aaaggtgcgt cctgcaatgg tgatgggact
     2101 gcaaagatgt ggaaatttgt gtgtcataat attttctctc cttttttatc aattcacgaa
     2161 ctcgcgctat cacgctctac tgcctgctat cgcaggcggt tcgttcatga atgacccaag
     2221 aaaaacaaat gccacctgcg gtgtcgcttg tttttctttt gggttcatac aatgtcattc
     2281 aattcttctt cgatcttctc ttcttccttt ttatcatctc cgtaaataag cacaggaagg
     2341 aaagaaagac gttttccaat acttcttgca ataaagccaa ctaaaacggc tgccacaata
     2401 gtaccttcac gtacaccctc taaatgaccg gaaaggagga atgaaagaac tgctgcgatc
     2461 actgccatgg aaacatcaaa acagaccttt gtaaaaccaa attcagaatg gatgcggaat
     2521 accacggctc ttacaaaaga ctctcccgga agcataacta catctgccag cacttccata
     2581 tatacgccaa cgccaaggac cagacagccg atcagaagtc ccaggacttt tgaaatatag
     2641 gataccggat gaacaaatcc cagaaggatc atagacatat caataaagta gccaaatgcc
     2701 atagataccg ggatctgcaa taaatgttct gccttaaaat tctttcccaa aagtaaaaac
     2761 tgaagaaaga tcagaagcaa actgaataca atggtgaagt ttcccagaga aaatgggaaa
     2821 ttcaggctga gtacataagg aatagaagaa ataggggaag ttcccagatt tcccttggtg
     2881 atcaggctga cgcccaacga gtttacaaac aggcccacta aaaagataag atagcgtttt
     2941 acctgctctg cttttgtacg tttcataatt cttgttccct ttcctgctta tttttcttta
     3001 tctgctcctt ttggaacatg tctttttcta tggtaacatc atcatataa
//
LOCUS       DBMDUA010000274         3037 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_11161_length_3037_cov_2.044619, whole genome shotgun sequence.
ACCESSION   DBMDUA010000274 DBMDUA010000000
VERSION     DBMDUA010000274.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11161_length_3037_cov_2.044619"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="ACZPCL_13235"
     CDS             <1..282
                     /locus_tag="ACZPCL_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR1163464.1"
                     /translation="KKMKTNAETSLKVLKDVLPILEAQEDFSNDALFAALSAYVSETG
                     VKTGFVMWPVRTAVSGKQMTPAGATEIMEVLGKEESLKRIRKGIEMLEA"
     gene            286..2160
                     /locus_tag="ACZPCL_13240"
     CDS             286..2160
                     /locus_tag="ACZPCL_13240"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR1163465.1"
                     /translation="MAFNEAQKRAIRHTDGPALVLAGPGSGKTTVITNRVRYLTEKVG
                     VEPGNILVITFTRAAAREMKERYERITGTGCSRVSFGTFHSVFFMILKLAYRYQASNI
                     VKEEQRIQFIREQLDQTDLEIEDEGEFISSVLSEISLIKGELMSLDNYYAKSCSQTVF
                     KELYDGYEHRLRKQGLLDFDDMLAMCYELFKERKDILQAWQRKYKYILIDEFQDINQI
                     QYEIVKMLALPENNLFIVGDDDQSIYRFRGARPELMLGFEKDYPDAERILLDVNYRCV
                     DPVIESAGKLIANNKTRFQKEIHGNRGKGCPVFVKEWPDPLAETKAITEELRDYHQMG
                     IAYEDMAVLYRTNQGPRLLIERMMEYNIPFHMRDTVPNLYEHWISRNVFCYIYAALGD
                     LSRSNILQIINRPARYISRDALDTKVIRWEQLRSFYQDKNWMLDRIDQLVYDLEMLRE
                     MAPAGAVNYIRKAIGYDDYLREYANERRLKPEDLFEVLDALQESAVPFKTYEAWFNHM
                     DEYKEQLKEQSALREAEKEGVSLMTMHSCKGLEFKVVYILDTNEGITPHHKAVLEPDL
                     EEERRMFYVAMTRAKDRLHIFYVKERYHKRQTVSRFVVETGLLGKKGDLEKNGKQGRK
                     "
     gene            2138..2707
                     /locus_tag="ACZPCL_13245"
     CDS             2138..2707
                     /locus_tag="ACZPCL_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR1163466.1"
                     /translation="MENREENKERESGKRTDGLVHIYCGDGKGKTTASVGAAVRAAGS
                     GKKVVIKRFLKNDHSGEVEVLKQIPGITVLPCTRQFGFSWKMSSQEKEEAKEYYGEEL
                     EKAWKTAWGQDADMLILDEAVGACSLGFIKEERLLELIGVKPEKLEVILTGRNPSKEL
                     LAAADYVTEMVMRAHPYTRGIPARKGIEY"
     gene            2797..>3037
                     /locus_tag="ACZPCL_13250"
     CDS             2797..>3037
                     /locus_tag="ACZPCL_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIR1163467.1"
                     /translation="MADEKNLNEEEEMTVTLTLDDDSEIECVVLNIFSAGDREYIALL
                     PMEGAESEEGEVYLYRYSETEDGTPVLDNIMDDDEY"
BASE COUNT          952 a          578 c          796 g          711 t
ORIGIN      
        1 aagaagatga agacaaacgc agagacttcc cttaaggtat taaaagatgt acttcctatt
       61 ctggaagcac aggaagattt cagcaatgac gctctgtttg cagcactttc cgcttatgta
      121 agtgaaactg gcgtaaagac aggctttgtt atgtggcctg tacgtaccgc tgtttccggc
      181 aagcagatga ctcctgcagg cgcaactgag atcatggaag ttcttggaaa agaagaatca
      241 ttaaaacgta tccgcaaagg cattgaaatg ctggaggctt aatacatggc ttttaatgaa
      301 gcacagaaaa gggcgatccg tcacacagac ggacctgccc ttgtacttgc aggacccggc
      361 tcaggtaaga ctacggtcat taccaaccgg gtccgttatt taacggaaaa agtgggcgtg
      421 gagcctggaa atatcctggt gatcaccttt accagggcgg cggcccgtga aatgaaagaa
      481 cgatatgaac ggataaccgg gacaggatgc agcagagtct cttttgggac cttccattct
      541 gtctttttta tgatattaaa actggcatac cggtatcagg cctccaatat cgtaaaagaa
      601 gagcagcgca tccagtttat cagggagcag ttagaccaga cagatctgga gatcgaggac
      661 gaaggagagt ttatttcttc tgtattgtca gagatcagcc ttattaaagg ggaattaatg
      721 tccttagata actattatgc aaaaagctgc tcccagaccg tctttaaaga actgtatgat
      781 ggctatgagc atcgtcttcg caaacaggga cttctggatt ttgatgacat gcttgccatg
      841 tgctatgagc tttttaaaga acgtaaggat attttacagg catggcagag aaaatataag
      901 tacatactta tcgatgaatt tcaggatatc aaccagatcc agtatgagat cgtaaaaatg
      961 ctggctctgc cggagaacaa tctgttcatc gtaggagatg atgatcagtc catttaccgt
     1021 ttccggggcg caaggccgga gctaatgctt ggatttgaaa aagattatcc agatgcagag
     1081 agaatcctgc tggatgtaaa ttaccgctgc gtggatccgg tgatcgagag cgcaggaaaa
     1141 ctgatcgcta ataataaaac acgtttccag aaagagatcc atggaaacag gggaaaaggc
     1201 tgtcctgtat ttgtaaaaga gtggccggac cctttagcag agacaaaggc tattacagaa
     1261 gaactgcggg attaccacca gatggggatc gcttacgaag atatggcggt tttataccgc
     1321 accaatcagg gaccaagact tctgatcgag cggatgatgg aatacaatat cccctttcat
     1381 atgcgggata cagtgccaaa tctttatgaa cactggatct ccagaaatgt gttctgctac
     1441 atttacgcgg ccttagggga tctgagccgt tccaatatac tccagatcat caaccggcct
     1501 gcaaggtata ttagccggga tgccttagat accaaggtga tccgatggga acagctgaga
     1561 tctttctatc aggacaaaaa ctggatgctg gaccgtattg accagctggt atacgacctg
     1621 gaaatgctgc gggaaatggc tccggcaggt gcagtaaact atatccggaa agctattgga
     1681 tatgacgatt acttaagaga gtatgccaat gagcgaagac taaagccgga ggacctgttt
     1741 gaggtcttag atgcccttca ggagagtgct gtgcccttta agacctatga ggcatggttc
     1801 aaccatatgg atgagtacaa agagcagcta aaggagcagt ctgccttaag agaggcagaa
     1861 aaagaagggg tcagtctgat gaccatgcac agctgtaaag gtctggaatt taaagtagta
     1921 tatatcctgg atacaaatga aggcataaca cctcatcata aggcggtttt agagccggat
     1981 ctggaagaag aacggcgcat gttctatgtg gcaatgacca gagcaaagga ccggctgcat
     2041 attttttatg taaaagagcg gtatcacaag cggcagaccg tatcccggtt tgtagtggag
     2101 acaggacttc ttggtaaaaa aggggattta gaaaaaaatg gaaaacaggg aagaaaataa
     2161 ggagagggaa tctggtaaaa gaacagatgg cctggtccat atctactgtg gggatggaaa
     2221 aggaaagacc actgcttctg taggtgcagc cgtccgggca gcaggaagcg gaaaaaaagt
     2281 ggtgataaaa cgtttcctga aaaatgatca ttccggggaa gtggaagttt taaaacagat
     2341 cccggggata acagtcctgc catgtaccag gcagtttgga ttttcctgga aaatgagttc
     2401 ccaggagaaa gaagaggcaa aggaatatta cggggaagaa ctggaaaaag cctggaaaac
     2461 agcatggggt caggatgcag atatgctgat cttagatgag gcggtaggcg cctgcagcct
     2521 tggctttata aaggaagaac gcctgctgga actgatcggt gtcaagcctg aaaaattaga
     2581 agtgatcctg acaggcagaa atccttctaa agaactgctt gcagctgcag attatgtcac
     2641 ggaaatggtc atgcgtgccc acccttatac caggggaata ccagccagaa aaggaattga
     2701 atattagctt gcaattttac aggcagtctg ataaagtgta aatgaacagc gaaaagctgt
     2761 aacatttaac cggattggat atgaaaggaa taaaatatgg cagacgagaa gaatttaaat
     2821 gaagaagagg aaatgaccgt taccctgaca ttagatgatg attctgaaat cgagtgcgtg
     2881 gtccttaaca tcttctctgc aggagaccgt gaatatatcg cccttcttcc tatggaagga
     2941 gcagagtccg aagaaggaga agtttatctg taccgttaca gcgagacaga agacggaact
     3001 cctgttctgg ataacatcat ggatgatgat gagtacg
//
LOCUS       DBMDUA010000275         2953 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_11598_length_2953_cov_2.040673, whole genome shotgun sequence.
ACCESSION   DBMDUA010000275 DBMDUA010000000
VERSION     DBMDUA010000275.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11598_length_2953_cov_2.040673"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="ACZPCL_13255"
     CDS             complement(<1..250)
                     /locus_tag="ACZPCL_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR1163468.1"
                     /translation="MLFDLEEELKKLPASPGVYLMHNNRDEIIYVGKAISLKNRVRQY
                     FQSSRNKTAKIQQMVSHIAWFEYILTDSELEALVLECNL"
     gene            complement(272..817)
                     /locus_tag="ACZPCL_13260"
     CDS             complement(272..817)
                     /locus_tag="ACZPCL_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HIR1163469.1"
                     /translation="MNRIPQPGTFYRHFKNKLYQVIAVATHSETGEKMVVYQALYGDY
                     QVYVRPLEMFISPVDREKYPDVEQEYRFEQVFPGQMNELGKNGNKPATGNLAGYDQTQ
                     QPEEPMSHEWLERFLDADRPEDQLAVLKQMEGHVTQKELDCIFLSLDLQPETGKTKTE
                     QVIDLRRHIQLLRRYDGSRLR"
     gene            complement(1001..2620)
                     /locus_tag="ACZPCL_13265"
     CDS             complement(1001..2620)
                     /locus_tag="ACZPCL_13265"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HIR1163470.1"
                     /translation="MHLLDSELKNKEQWEKAEISLPQFDRQAMKEETKKNPQWVHFGA
                     GNIFRAFPAALQQKLLNEGIEKTGIIVAEGYDYEIIKKAYRPQDDLSLLVTLKANGTI
                     EKTVIGSLAESLTVDRHDAADWNRLKEIFASNTLQMVSFTITEKGYSITSPDGAFRAD
                     VKADFEAGPENADSYIGKLAALCYWRYTQGAAPLALVSMDNCSHNGDKLFAAVDAYAK
                     AWTENGKAEKGFLEYIENPAKVSFPWSMIDKITPRPDASVKKMLEDAGFTDTESIVTS
                     KNTYVAPFVNAEEAQYLVIENAFPNGRPALENAGVMFTSRETVEKVERMKVCTCLNPL
                     HTALAVYGCLLGYTKISDEMKDADLVKLVEIIGYKEGLPVVTDPGILSPKKFIDEVLQ
                     IRVPNPFMPDTPQRIACDTSQKLSIRFGETIKEYMASSELDVKSLKFIPMVQAGWCRY
                     LLGVDDELNAFTVSPDPIYEELAGKLAGVKVGESCDVHAVLSPILSDAKIFGVNLYEA
                     GLGEYVESIFAELIAGKGAVRETLKRHLAEA"
BASE COUNT          706 a          726 c          583 g          938 t
ORIGIN      
        1 ttaaattgca ctctaagacc agcgcctcca actcagagtc tgtcaggata tattcaaacc
       61 atgcaatatg ggataccatc tgctggatct tggctgtttt attgcggctg ctctgaaaat
      121 actggcgtac acgatttttc aaactgatgg ccttgcctac ataaatgatc tcatcccggt
      181 tattgtgcat cagatataca ccggggcttg caggcagctt tttcagttct tcttccagat
      241 caaatagcat tcgttcacct cattttaacc atcagcgcag tctgctgccg tcataccggc
      301 gcagcagctg tatatggcgg cgcagatcta ttacctgctc tgtttttgtc tttcctgttt
      361 ccggctgcag gtccaaagac agaaagatac agtccagttc tttctgtgtt acatggcctt
      421 ccatctgttt taatacagcc agctgatctt ccgggcggtc agcgtccaga aaacgttcca
      481 gccattcgtg gctcatcggt tcttctggtt gctgggtctg atcatatcct gccagattgc
      541 cagtggccgg cttatttcca ttttttccca gttcattcat ctgtcctgga aatacctgct
      601 caaaacggta ttcctgctcc acatccggat atttttcccg gtctaccggg cttataaaca
      661 tctccaacgg tcttacatac acctggtaat ccccatagag agcctgatag accaccattt
      721 tctctcctgt ttctgaatga gtggccactg caatgacctg atataattta tttttaaaat
      781 gcctgtaaaa agttccaggc tgtgggatcc tgttcatggt tatactcctt ctgtccgtac
      841 atatgtttcc ctgctagtat aacacatgtc ttgggaatat aaaaggcgct tctttcagat
      901 gcatggcagc aaaaaaggcc gcaacatttc tgccacggcc agttttaacc tatttataac
      961 acaaactttt atttacctgt tgatgcaggt aagccttatc ttatgcctca gccagatgtc
     1021 tctttaaagt ctctcttaca gcgccctttc cagcgatcag ttctgcgaag atgctttcta
     1081 catattcgcc aagacctgcc tcatataagt ttacaccgaa gatctttgca tcacttaaga
     1141 ttggggaaag aacagcgtgt acgtcgcagg actctcctac ctttacgcct gccagcttgc
     1201 cagccagttc ctcataaatt ggatccgggc ttactgtaaa tgcgttcagt tcatcatcta
     1261 cacctaacag ataacggcac catccagcct gtaccattgg gatgaacttt aaggatttta
     1321 catccagttc agagcttgcc atgtattcct taatggtctc accaaagcgg atggacagct
     1381 tctgggaagt atcgcaggca attctctgtg gagtatcagg cataaatggg tttggtacac
     1441 gaatctgaag tacttcatca ataaacttct ttggagaaag aattccagga tctgtaacaa
     1501 ccggaagtcc ctctttgtat ccaatgatct caaccagttt aaccaggtca gcatccttca
     1561 tctcatcgct gatcttggtg taacctaaca ggcagccata tacagccagt gcagtgtgca
     1621 gaggatttaa gcaggtgcat actttcatgc gctctacttt ttctactgtc tctctggagg
     1681 tgaacataac acctgcattc tctaatgccg ggcggccgtt tggaaatgca ttttcaatta
     1741 caaggtactg agcctcttct gcgtttacga aaggtgctac gtaggtattc ttgcttgtta
     1801 caatgctttc tgtatcagta aagccagcat cctctaacat cttctttacg cttgcgtcag
     1861 gacgtggggt gatcttatcg atcatgctcc aagggaagga aacctttgca gggttctcaa
     1921 tgtactctaa gaagcccttc tctgcttttc cgttctcagt ccatgctttt gcataagcat
     1981 ctactgctgc gaacagctta tctccgttgt gggagcagtt gtccatactt accagtgcca
     2041 gtggagctgc gccctgggta tatctccagt agcataatgc tgccagctta ccgatgtagc
     2101 tgtctgcatt ttcaggacct gcctcaaaat cagcctttac atctgcgcgg aatgcaccgt
     2161 caggagaagt gatactgtag cccttttcag taatggtaaa gcttaccatc tgaagggtat
     2221 tgcttgcgaa gatctctttt aaacggttcc agtctgctgc gtcatgacgg tctacagtca
     2281 gtgactctgc aaggcttccg atcacggtct tttcaatggt tccgtttgcc tttaaggtaa
     2341 ctaaaaggct taaatcatcc tgtggacggt atgctttctt gatgatctca taatcataac
     2401 cctctgctac gatgatacct gttttttcaa ttccttcatt taacagtttc tgctgtaatg
     2461 cagctgggaa tgcgcggaaa atattacctg cgccaaagtg tacccactgt gggttcttct
     2521 ttgtctcttc cttcatagcc tgacggtcaa actgtggaag actgatctca gccttctccc
     2581 actgttcttt gtttttcagt tcgctgtcca gtaaatgcat aatgataccc tcctgttttg
     2641 gttttatatt actgttatac taatatatta cttcttaact ctgtctctat tctaccacat
     2701 atttttttat tttccatatg aatttttgtc aatatcattg caaaagttgc gttttctatg
     2761 taaactgtga aaaatcccga tctcagcctg ctgcaacaaa cttcctcatg gcggaaatgc
     2821 ccattgttgg cgcaaaaaag aatgtggctt ggcataaaaa ataggcttcg cctattcaac
     2881 tcccctgccc ctcaatcttg aggggttagg ggtttctttt ttctaaaatc tatctcataa
     2941 tagtcttatg aat
//
LOCUS       DBMDUA010000276         2950 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_11612_length_2950_cov_2.743419, whole genome shotgun sequence.
ACCESSION   DBMDUA010000276 DBMDUA010000000
VERSION     DBMDUA010000276.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11612_length_2950_cov_2.743419"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            280..954
                     /locus_tag="ACZPCL_13270"
     CDS             280..954
                     /locus_tag="ACZPCL_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012885.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163471.1"
                     /translation="MLLRGQNLIGYKQYPDDVVEKFVELAAKAGIDKFLVFDGLNDVR
                     NTETAIKAVVKNGKVAEANICYTISPVHTLEKYVQMAKDYAALGVKAIHIEDMAGMIN
                     PKQVSDVISAVRKEVGLPVHFHAHCIGGMADICYWEAIKAGACTVDCDVSSLSLSPSH
                     PPLESIVTCLKGTGFDPHLDMDLLAQINDYFKMIREKYKEYASNRGTGSIQYEHSRQR
                     NKYIHL"
     gene            1085..1558
                     /locus_tag="ACZPCL_13275"
     CDS             1085..1558
                     /locus_tag="ACZPCL_13275"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR1163472.1"
                     /translation="MAPAEEEKTETRSLTFEGRCWKFGDDIDTDIIIPTQHLNYETMD
                     EIKTHVFEPLRPDLAAKLREGDLIVAGSNFGCGSSREQAAEVLVANGILMLECQDLAD
                     HVKEGDIIQVDLDNQKITADGKTYAIPAIPENLYHIIMDGGLVKSIMKKVEKGLL"
     gene            1575..2906
                     /locus_tag="ACZPCL_13280"
     CDS             1575..2906
                     /locus_tag="ACZPCL_13280"
                     /inference="COORDINATES: protein motif:HMM:NF015355.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HIR1163473.1"
                     /translation="MGKKVEFKVCGLTAKYFVPLFLVVIICTYGGFMPTVKIGETTGV
                     GMVGTLAFLMSVGGLFFWLGNSIPIVNSYLGGSVLFCMFGPALLNYFGLLPETTVKGV
                     SILMKYGFQDVYIAALLCGSVLYMDRKILLGAISRYLPAILGSQVFALGFCFLGGLIT
                     GYGPLNAIFDIGAPTMSGGSGGALVTIPILYTDLSGTDWMSMSGMFLCYVSLSNVVAV
                     LMCALSKPILRKLGMVSPDENPAILRKAGTMQTESTEQLPALSEDPAKVCGGIFICLG
                     IYLLGTILGKLPGTKVIAGLAWAIIIAVVVKCTGIMDEKITNYTVNGMNFMLKGLLPA
                     IIAGIGINSISIETMISYFTPAALVIIVLAVLGAYIGAIIFGQLFGLWGYEAGITAGL
                     CCCNIGGSGDLAVLTAGDRMSLLAFSSISTRIGGALMVVWIGFLYPLLMLK"
BASE COUNT          821 a          580 c          710 g          839 t
ORIGIN      
        1 cttcatccag aggttgcaat agcataaagc actattttgt tagctgaaat attgaatttt
       61 caaggtgcga agccctgttt aggtgtggga agttttagtt aataatttaa ccaataatac
      121 caagatataa gtttgcatat taacgttaca gttaacaaaa gtaacaattg ataactaagg
      181 aaacgacttg gttgatgata tgcaaacgga tatagggggg atgatccctg ggatcgtgta
      241 agagagttta agaagctttg taagaagact ccgctaagaa tgcttcttcg tggacagaat
      301 ctgatcggct ataagcagta tccagatgat gttgtggaga agtttgtaga actggctgca
      361 aaagcaggaa ttgacaagtt cctggtattt gacggcttaa atgatgtgag aaatacagag
      421 acagctatta aggcagttgt taaaaacggc aaggttgctg aggcaaatat ctgctatacc
      481 atcagtccgg tacatacatt ggaaaaatat gtgcagatgg caaaggacta cgcagcattg
      541 ggcgtaaaag ctattcatat tgaagatatg gcgggaatga taaatccaaa gcaggtttct
      601 gatgtgatca gcgcagtgcg caaagaagta ggacttcctg ttcattttca tgcacactgc
      661 atcggtggca tggctgatat ctgttactgg gaggccatta aggctggtgc ctgcacagta
      721 gactgcgatg tttcctcctt atctttaagc ccatctcatc cgccgcttga aagtattgtt
      781 acctgcttaa agggaaccgg atttgatcct catctggata tggatcttct ggcccagatc
      841 aatgattatt ttaaaatgat ccgtgagaaa tataaagaat atgcaagtaa caggggaacc
      901 ggcagcattc aatacgaaca cagcagacag cgaaacaagt acatacatct ttgatgcagt
      961 taaaacccgg gaagtaacag cacttccagg tggctgcaca gagatcaaac ctctctctga
     1021 actgtcagca gtaaagatca aaagtgtatt catcggcgga accactggcg gcaccttaaa
     1081 agaactggct ccggcagagg aagagaagac agaaacacgt tcccttacct ttgagggccg
     1141 ctgctggaag tttggtgatg atattgacac cgatatcatt atcccaacac agcacttaaa
     1201 ttatgagaca atggacgaga tcaaaaccca tgtatttgag cctttaagac cggatctggc
     1261 agcgaagctt cgggaaggtg acctgatcgt agcaggaagc aacttcggct gcggttcctc
     1321 cagagagcag gcggcagagg ttctggtggc aaatggtatc ctgatgcttg aatgccagga
     1381 tctggcggat cacgtaaagg aaggtgatat cattcaggta gatctggaca accagaagat
     1441 cactgctgac ggcaaaacat atgcgattcc ggctatcccg gaaaacctgt accacatcat
     1501 tatggatggt ggtctggtaa aaagcattat gaaaaaagtt gaaaaaggtc tgttatagaa
     1561 aggaggaaat caaaatgggt aaaaaagtgg aatttaaagt ctgcggactt acagcaaagt
     1621 acttcgtacc actgttttta gtagtgatca tctgtactta tggcggcttt atgccaactg
     1681 taaagatcgg tgagactacc ggtgttggaa tggtgggaac actggccttc ttaatgagtg
     1741 taggcggcct gttcttctgg ttgggcaaca gtatccccat tgtaaacagc tacttaggcg
     1801 gctctgttct tttctgtatg ttcggaccgg cattgttaaa ttatttcgga ctgcttcctg
     1861 aaacaactgt aaagggcgta tctatcctta tgaaatacgg ctttcaggac gtatacattg
     1921 cagcccttct ttgtggatct gtcctgtata tggaccgtaa gatcttatta ggtgctattt
     1981 cacgttatct gcctgcaatt ttaggctctc aggtatttgc actgggattc tgctttttag
     2041 gtggtcttat cacaggatat ggcccattaa atgctatctt tgatattggt gcgcctacta
     2101 tgtccggcgg ctccggcggt gctcttgtaa ctattcctat tctgtatacg gatctttccg
     2161 gtacagactg gatgagcatg tccggtatgt ttttatgcta cgtttcactt tctaacgtag
     2221 tagctgtttt aatgtgtgct ctttccaaac caattttaag gaagctgggg atggtatctc
     2281 cagatgaaaa tccggctatc cttcgcaaag caggaactat gcagacggaa agcactgagc
     2341 agcttccagc cctcagtgaa gatcctgcca aagtctgcgg cggtatcttt atctgccttg
     2401 gcatctattt attaggaact attttaggca agcttcccgg aaccaaagtg atcgctggtc
     2461 ttgcatgggc gatcatcatt gctgttgtgg tcaaatgcac cggtattatg gatgaaaaga
     2521 tcaccaacta cacagtaaat ggcatgaact ttatgttaaa gggactgctg ccagcgatca
     2581 tcgcaggtat cggcattaac tctattagta ttgaaactat gatcagctat tttacaccag
     2641 cagctttagt tatcattgtt ttagcagttc ttggtgcata cattggagcc atcatctttg
     2701 gacaactttt tggtctgtgg ggctatgaag ccggtattac agcaggtctt tgctgctgca
     2761 acatcggtgg ttccggtgat cttgcagtgc tgacagcagg tgacagaatg agcctgttag
     2821 cattctcctc tatttccacc cgtatcggcg gtgcgctgat ggtagtatgg atcggtttct
     2881 tatatcccct tttaatgtta aaataatcga atagtcattt tcatgtaaac tcaagcaaca
     2941 ggtattgtct
//
LOCUS       DBMDUA010000277         2893 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_11867_length_2893_cov_1.927650, whole genome shotgun sequence.
ACCESSION   DBMDUA010000277 DBMDUA010000000
VERSION     DBMDUA010000277.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11867_length_2893_cov_1.927650"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            80..334
                     /locus_tag="ACZPCL_13285"
                     /pseudo
     CDS             80..334
                     /locus_tag="ACZPCL_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            355..804
                     /gene="flgC"
                     /locus_tag="ACZPCL_13290"
     CDS             355..804
                     /gene="flgC"
                     /locus_tag="ACZPCL_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HIR1163474.1"
                     /translation="MAFLGSFDISASGMSAQRVRMDIAAENIANMDTTRTESGGPYRR
                     KNVLLESYSDTSFAQAMENAARGKGITSRHAGVRVSGIVEDDREAKKVYNPDHPDADA
                     DGYVTMPNVDVLKETVDSMSATRSYEANVTALNAIKLMAQKALEIGK"
     gene            825..2108
                     /gene="fliY"
                     /locus_tag="ACZPCL_13295"
     CDS             825..2108
                     /gene="fliY"
                     /locus_tag="ACZPCL_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HIR1163475.1"
                     /translation="MGALSFNEMEIDALGEMMNISLGASATAMSTLLGSTVHITTPKV
                     KILTRDQFEFKKLEPAVGVEIAYVEGLEGSNIMMFSRNDVRIIVGTLLGEEIPDDKFE
                     LDEINRSAICEVMNQMMGASATALSEFLKFTVNISTPVSFEITDAESFKNKYFPEATP
                     MVVISFILEIEGKLKSEFLNIMPEKLAKRLLEPFEISLEEAAEIPAENKESGSKAAEE
                     AAEKVIEEAAEKEKPQPEPEPAPTPQPAPTPQPAPTPQPAPAPQPAPTSQPVYSQIPA
                     GISADMNPAILQLLNQMQQSQVQMMQLIQDMEKREQDEKKESREPALIRPANAPGLES
                     SGSENGEENANQEMLMKVPLEISVEIGRTKKLVKDILEFTQGSLVVLDKMAGEQADLY
                     VNGQCVARGDIVVVEDNFGIRITDIVTRDINPESL"
     gene            2110..2376
                     /gene="fliO"
                     /locus_tag="ACZPCL_13300"
     CDS             2110..2376
                     /gene="fliO"
                     /locus_tag="ACZPCL_13300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HIR1163476.1"
                     /translation="MITDIVSTTGMLLAFVVILFLAWQASRYLGKNASYGKKGGRMQI
                     LEQLKVGNDQYVILLKLDETQYLLGTSQTGIQVLERLKEENDHE"
     gene            2369..>2893
                     /locus_tag="ACZPCL_13305"
     CDS             2369..>2893
                     /locus_tag="ACZPCL_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HIR1163477.1"
                     /translation="MNELITGLNGGNVPALDIIMLLTFAALLPSIVVMMSSFMRIIII
                     LSFTRNAMGVQQTPPNIVLTGMALFLTLYIMSPVIGQINTVAYEPYKNGEITQQEAVS
                     RMADPLKQFMLRNTEKDTLDHFVQMSKQEVKKDVKEYPLTVVVPAFMTSELKKGFLAG
                     FLIYLPFLLIDIVVS"
BASE COUNT          930 a          547 c          727 g          689 t
ORIGIN      
        1 caccgtatgt atagaccacg gtctacaacc taaatccgga agctatgcat atatagtact
       61 acctgataaa aagactcccg aagcagtaaa aaaatacagt gaggaaaaac cggacatggt
      121 gatcatggat atcaccatgc caaatatgga cgggcttcag gctttgaaaa aaattcgtga
      181 agatgatcca aatgccaaag ttgttatgtg tacagcaatg ggacaggaaa gcatggttgt
      241 agatgcgatc aaatcaggtg cccgtgattt tgtggtgaag ccttttgatg cacagagaat
      301 tgtaaaaact gtaaatgaca ttcttggaaa ttgatccata aggagggaac tgttatggca
      361 tttttaggtt catttgatat aagtgcttct ggaatgagcg cgcagcgtgt caggatggat
      421 attgcggcag aaaacatagc aaatatggac acaactcgga cagagtcagg tggtccatac
      481 cgaaggaaaa atgtacttct tgaaagctat tcagatacgt cttttgccca ggcaatggaa
      541 aatgcagcac ggggaaaagg aataaccagc cgtcatgcag gagtaagagt ttctggcatt
      601 gtggaagatg acagagaagc taagaaagtt tacaatccgg atcatccgga tgcggatgcg
      661 gacggctatg tgactatgcc aaatgtggat gttctgaagg aaacggtaga cagtatgtca
      721 gctacccggt cttatgaagc aaatgtaaca gcattaaacg caattaagct gatggctcag
      781 aaagcactgg aaattggtaa gtagaaaaca ggaggaaaat gagaatgggt gcactcagct
      841 ttaatgagat ggaaatagat gctcttgggg agatgatgaa tatcagcctt ggagcatcag
      901 cgacagcaat gtctacactt ttaggatcta ccgttcacat tacgactcca aaggtaaaga
      961 tccttacaag ggatcagttt gaatttaaaa agctggaacc ggcagtagga gtagagatcg
     1021 cttatgtaga aggactggaa ggaagcaata ttatgatgtt cagcagaaat gacgtgcgca
     1081 tcatagtagg aacccttctg ggagaagaga taccagatga taaatttgaa ctggatgaga
     1141 tcaacagaag tgctatttgt gaggtaatga accagatgat gggtgcatca gcgacggcac
     1201 tttcagagtt tctgaaattt acagttaata tatccacacc ggtttctttt gagatcacag
     1261 acgcagaaag ctttaaaaac aagtatttcc cggaagcaac tcctatggta gtcatttcct
     1321 ttatccttga gatcgaagga aaactgaaaa gtgagttttt aaatatcatg ccggaaaagc
     1381 ttgcaaaaag gttactggaa ccatttgaga tatccctgga agaagccgca gagatcccgg
     1441 cagaaaataa ggagtcagga tcaaaggcag cagaagaagc tgcagagaag gtgatagaag
     1501 aggcagcaga gaaagaaaaa ccgcagcctg agccagaacc agcaccgacc ccgcagccag
     1561 caccgacccc gcagccagca ccgaccccgc agccagcacc ggcaccgcag ccagcaccta
     1621 catcgcagcc tgtatattcc cagattccag ctggtatttc cgcagatatg aacccggcta
     1681 ttttacagct tttgaatcag atgcagcagt cacaggtgca gatgatgcag ctgatacagg
     1741 atatggaaaa acgggaacag gatgagaaaa aggaatccag ggaacctgcc ctgatccggc
     1801 cggccaatgc acctggtctt gaatcttctg gaagtgaaaa tggagaagaa aatgccaacc
     1861 aggaaatgct catgaaggtg ccgttggaga tatcagtgga aataggacgc actaagaaat
     1921 tggtaaagga tattctggaa tttacccagg gatctcttgt agtcctggat aaaatggcag
     1981 gagaacaggc agacctgtat gtaaatggac agtgtgttgc caggggagat attgtagtag
     2041 tagaggataa ttttggtatc cgtattaccg atattgtaac cagggatatc aatccggaga
     2101 gcctttaata tgataacaga tattgtttct acaacaggta tgctgcttgc ttttgtggtc
     2161 attttgtttc tggcatggca ggcaagccgc tacctgggaa aaaatgcctc ctatggaaaa
     2221 aaagggggaa gaatgcagat actggaacag ctgaaagtag ggaacgatca gtacgttatc
     2281 cttcttaagc tggatgaaac ccaatatctg cttggaacca gtcagacagg gatacaggtt
     2341 ctggaacggc ttaaggagga gaatgatcat gaatgaactg attacaggct taaatggagg
     2401 aaatgttcca gcactggaca ttatcatgct gctcaccttt gcagcactgc ttccttccat
     2461 tgtggttatg atgagttcct ttatgcgtat cataatcata ctttccttta cgcgaaatgc
     2521 aatgggagtc caacagacgc cgccgaatat tgtcctgaca ggaatggcac tttttttgac
     2581 actgtatatt atgagtccgg ttataggcca gatcaatacg gttgcctacg agccctataa
     2641 aaatggggag atcacgcagc aagaggcagt aagccgtatg gcagatccac tgaagcaatt
     2701 catgcttcgc aatacagaga aggatacgct ggatcatttt gtacagatgt caaaacagga
     2761 agttaagaaa gatgtgaaag aatatccgct tacagttgtt gtaccagctt ttatgaccag
     2821 cgaattaaaa aaagggtttc tggcaggatt tctgatctat cttccattcc tgcttataga
     2881 tatagtggtg tct
//
LOCUS       DBMDUA010000278         2870 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_11968_length_2870_cov_1.738895, whole genome shotgun sequence.
ACCESSION   DBMDUA010000278 DBMDUA010000000
VERSION     DBMDUA010000278.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11968_length_2870_cov_1.738895"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            105..1166
                     /locus_tag="ACZPCL_13310"
     CDS             105..1166
                     /locus_tag="ACZPCL_13310"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HIR1163478.1"
                     /translation="MARLRMMRAPQKYVQGKDSLFKFHEEMKDLGNKFLFVCSRSGYK
                     MCHDKIEKSFEGTEDIRQYEIFGGVSSVGEIERMRKIVRENRLNVVVGVGGGSAIDTA
                     KATAHYEKLPVVIIPTVVATDAPCTGLSVIYNDDETFNSYLFYPKNPEAVIVDSDVIA
                     KSPVKFLVAGMGDALGTYFEARACQRTDAPSLEFGGITRSAMALCKLCYETLKEYGAA
                     AKHACENQVVTPALDAIIEANVYLSGVGADNGGLAVAHSFYNGLTALGGHSAPHGNCV
                     AYGTLVQLILEGAPKAEFKEVQDFCREVGLPVTLEEIGVTTKEQVKVIAEHACVEGES
                     IHNGSRCNCRSALRCNPCC"
     gene            complement(1206..2072)
                     /locus_tag="ACZPCL_13315"
     CDS             complement(1206..2072)
                     /locus_tag="ACZPCL_13315"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1163479.1"
                     /translation="MDEQDIELLKTLIDTQNITKTAKKLYTTQSSLTKRIQKMEQDLG
                     CQLFIRSRKGILPTPAAEGIFPEIEKISQSMEHIRAYALSLQGEICGSLKIGVSVNIA
                     RYKLPAVLKTFMKKYPKVDIYVTTMQSTQLYRALADNEISIALVRGNFNWQDGDVILS
                     REPVCLVRNQEMAELSLKDIPYIGRHTDSPFYGRIEQWKQENGCANCRTQLWIDDISS
                     CLEMVENGIGWSILPEICLKNYKGSITPLFFQDGSPFIRTSHILYKSHYFELPQVRAF
                     IQTVLAEEYFNA"
     gene            2238..>2870
                     /locus_tag="ACZPCL_13320"
     CDS             2238..>2870
                     /locus_tag="ACZPCL_13320"
                     /inference="COORDINATES: protein motif:HMM:NF016210.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HIR1163480.1"
                     /translation="MYMKKYPCVYMRGGTSKAVFFHEKDLPEDQSLWDDIFMKVMGTP
                     DVKQIDGMGGTVSSTSKVAIISKSDRPDVDVNYTFRQVDIIIPRVDGSANCGNISSAV
                     GPFAIDEGLVPAVEPITVVRILNTNTNKIIEEHVRVKDGHADVYGDEVIKGVPGTGSR
                     IDMYFEDPAGSKTGKLFPTGQKKEIFDVPGYGPAEVTVLDCSNPMVFIKAS"
BASE COUNT          866 a          527 c          681 g          796 t
ORIGIN      
        1 tatttcttgg cattttaggg tgactggtag tataatatat gcatgtgata ataaattaac
       61 aaactaatga tctcaaaata atgtgaaaac aggaggtaat tattatggca agactgagaa
      121 tgatgcgtgc gccgcagaaa tatgtacagg gaaaggattc tctgtttaaa ttccatgagg
      181 aaatgaaaga tcttggaaac aaattcttgt ttgtctgttc aagaagtgga tacaaaatgt
      241 gccatgataa gattgagaag agttttgaag gaacagaaga tatccgccag tatgagattt
      301 ttggtggtgt atccagtgtt ggtgagattg aacggatgag aaaaattgtc cgcgaaaata
      361 gactgaatgt agtagtaggt gttggcggtg gttcagccat tgatacagca aaagctactg
      421 ctcattatga aaaacttccg gtggtaatta tcccaacagt cgttgcgaca gatgctccat
      481 gtacaggact ttctgttatt tacaatgatg atgagacatt taacagctat ttattttatc
      541 caaagaaccc ggaagctgtt attgtggaca gtgatgtgat cgcaaaatcg cctgttaaat
      601 ttttggtagc aggtatgggt gatgctcttg gaacttattt tgaagcaaga gcatgtcagc
      661 gtaccgatgc gccaagcctg gaatttggtg gtattacaag atctgcaatg gcactgtgca
      721 aactttgcta tgagacttta aaagagtatg gcgcagctgc aaaacatgcc tgtgaaaatc
      781 aggtggttac tccagcgttg gatgctatca tcgaagccaa tgtatacctg tctggtgtag
      841 gcgctgataa tggcggtctg gctgttgccc attcattcta taatggtctg acagctttag
      901 gcggacacag tgcaccacat ggaaactgcg ttgcttatgg tactctggta cagctgatat
      961 tagagggcgc tccaaaggca gagtttaaag aagttcagga tttctgccgg gaagtaggtc
     1021 ttcctgttac tctggaagaa attggtgtaa caaccaagga acaggtaaag gtcattgcag
     1081 agcatgcctg tgtagaaggc gaatccatac ataatggtag cagatgtaac tgcagatcag
     1141 ctttacgatg caatccttgc tgctgattct ctggggaaag aggtactggg aaaatagtgc
     1201 tgtatttaag cattaaagta ttcctcagca agcacagtct gtataaaggc ccgcacctga
     1261 gggagttcaa aataatggct tttatataaa atatgagaag tacgtataaa gggagatccg
     1321 tcctgaaaga aaaggggcgt gatgctccct ttataatttt ttaaacagat ctccggcaaa
     1381 atagaccagc caataccatt ttccaccatt tccaggcagc tggaaatatc atcgatccaa
     1441 agctgggtgc ggcagttggc gcacccattt tcctgtttcc actgttcaat gcggccgtaa
     1501 aaaggagagt ccgtatgacg tccgatataa gggatatcct ttaaggagag ttcagccatt
     1561 tcctgatttc gtaccagaca taccggttct ctagaaagga taacatcccc atcctgccag
     1621 ttaaaattgc cacggaccaa tgcaatagag atctcattat cagccagtgc acggtataac
     1681 tgggtacttt gcatggtggt tacgtaaata tctacttttg gatatttttt cataaatgtt
     1741 ttcagtacag ctggaagttt ataccgggca atattaacag aaacaccgat ctttaaagaa
     1801 ccgcagatct ccccctggag cgaaagggca taggcccgta tgtgttccat ggactgggag
     1861 atcttttcaa tttccgggaa gatcccctca gcagcgggtg tagggagaat gcctttgcgg
     1921 gaacggataa aaagctggca tcccaggtcc tgctccattt tctggattcg tttggtgaga
     1981 gaagactggg tggtataaag cttctttgct gtctttgtaa tattctgtgt atcgatcaat
     2041 gtttttaaaa gttcaatatc ctgttcatcc ataaataaac caacctcctg tcaggcaaat
     2101 ttccataaac ataaaccatt ccataatgga atgcaatata atgaaattat acaattttac
     2161 agaatggata gcaaggccta taatacagtt aaacaaagaa aaacgtatag aaatcaatat
     2221 aaaggaggac ttgttttatg tatatgaaga aatatccctg tgtttacatg agaggcggta
     2281 ccagcaaagc cgtatttttc catgagaagg atctgccgga agatcagagt ctctgggatg
     2341 acatttttat gaaagtcatg ggaaccccgg atgtaaaaca gatcgatgga atgggcggaa
     2401 ccgtatcatc tacaagtaag gttgccatta tttccaaatc cgaccgcccg gatgtagatg
     2461 taaattatac attccgccag gtagacatta tcattccaag ggtagatgga agcgccaact
     2521 gcggaaatat ctcatctgct gtaggtccat ttgccattga tgagggcctg gttccggctg
     2581 tagagccaat cacagtagtt cgtattttaa ataccaatac caacaagatc attgaagaac
     2641 atgtacgtgt aaaagatggc catgcagatg tgtatgggga tgaagtgatc aagggtgttc
     2701 ctggaacagg ttcccgtatt gatatgtatt ttgaagatcc ggcaggttct aaaacaggaa
     2761 agctcttccc cacaggacag aaaaaagaga tttttgatgt accgggatat ggtccggcag
     2821 aggtaacggt tttagactgc tccaatccta tggtatttat caaggcatcg
//
LOCUS       DBMDUA010000279         2844 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_12106_length_2844_cov_1.902661, whole genome shotgun sequence.
ACCESSION   DBMDUA010000279 DBMDUA010000000
VERSION     DBMDUA010000279.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12106_length_2844_cov_1.902661"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(108..1106)
                     /locus_tag="ACZPCL_13325"
     CDS             complement(108..1106)
                     /locus_tag="ACZPCL_13325"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012407.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR1163481.1"
                     /translation="MADTITVFKGDGIGPEITDSVLRILDAAGADLNYEVFNVGAAEY
                     EAHGELIPKEGYASMEKTHILLKSPITTPLGKGFRSLNVTLRKKYDLYANIRPAKTNS
                     AVKTAFENVDIVIFRENTEDLYAGVEEMIDKDTVHSIKIITRRCCERIIRAAFDYAVS
                     HNRKKVTCVHKANIMKMADGMFRDIFYDISKEYPDIEADDKIVDNVAMQLVMHPEKFD
                     IIVTENLYGDVLSDLVSGLIGGLGLLPSSNLGKDFAMFEAVHGSAPDIAGQGIANPTA
                     FLWSACMLLEHIGKNDVAAKIRKAVDLVLEDGSVLTPDIGGTATTIEYEKAIIAHL"
     gene            complement(1109..2449)
                     /locus_tag="ACZPCL_13330"
     CDS             complement(1109..2449)
                     /locus_tag="ACZPCL_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR1163482.1"
                     /translation="MKDSYLAQFYGKSQNYTDIPNKWYKEYNVKKGLRNEDGTGVRVG
                     LTRVADVVGYDETPEGGIQAIPGKLYYRGYDVEDLVKGKGDSHFGYEEVCFLLLFGYL
                     PKKAELEHFRNILTDCYEIPDEFVEMNLLRMPGRNLMNKLQDAVLTLYNYDDDPDNTD
                     VYETLVKGINLMAKLPSIACYAYQSKVHYYNRDSLFIHYANPKYSIAENFLSLLRKDQ
                     KFSDKEARLLDTILMLHADHGGGNNSTFTNVVISSTGTDIYSTVCGSIGSMKGPRHGG
                     ANIKVAEMMDAVLEETGGYTADDKKIKTAVNKLMDGELYDRTGLVYGFGHAVYTISDP
                     RAEMLRARCLEVAKEKKMMTEFDFRQRFERIALETMKERKGVALPTNVDYYSGFAYEM
                     LGIPRDLYTPLFVCSRMVGWVAHNIENKLYDGRIMRPATKYVGDNKEYTAMEER"
     gene            complement(2504..>2844)
                     /locus_tag="ACZPCL_13335"
     CDS             complement(2504..>2844)
                     /locus_tag="ACZPCL_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HIR1163483.1"
                     /translation="YAASCQRVIGGLANICKEYATKRYRSNVMNWGMLPFQMEADPDF
                     EVGDYIYVPGVRKALDGDLSSIKAYVLGNGKDAKAEIKELNLYIADMTPEERAIVKAG
                     CLINYNRNRH"
BASE COUNT          741 a          679 c          525 g          899 t
ORIGIN      
        1 cttcatccag aggttgcaat agcataaagc actattttgt tagctgaaat attgaatttt
       61 caaggtgcga agccctgttt aggtgtggga agttttagta attaatttta cagatgcgca
      121 ataattgcct tctcatattc aatggttgta gcagttccgc cgatatctgg tgtaagaaca
      181 cttccgtcct ccagtaccag gtctactgcc ttgcggatct tggcagcaac atcattcttt
      241 ccgatatgct ctaacagcat acatgcggac cataagaatg cagttgggtt tgcaattccc
      301 tgtcctgcaa tatcaggggc actgccatga actgcctcga acatggcgaa atctttaccc
      361 aggttgctgg atggaagaag tcccagacct ccaatgaggc cgctgaccag atcagataaa
      421 acatcaccat acaggttttc tgttacgata atatcaaatt tttccggatg cataaccagc
      481 tgcatagcca cattgtctac aatcttatca tctgcttcaa tatccggata ttccttgctg
      541 atatcataga agatatcgcg gaacattcca tctgccattt tcatgatatt tgccttatga
      601 acgcaggtca ctttcttacg gttatggctt actgcatagt cgaaagcagc gcggatgata
      661 cgctcacagc atcttctggt aatgatctta atggagtgaa ccgtatcctt atcaatcatc
      721 tcttccacac ctgcatacag atcctctgta ttttcacgga agattacgat atctacattt
      781 tcaaatgcag tctttacagc agagtttgtc tttgcaggac ggatatttgc gtaaagatca
      841 tatttctttc ttaaagtaac atttaaagaa cggaagccct ttcctaaagg agttgtaata
      901 ggggacttta ataaaatgtg ggtcttttcc atagaagcat agccctcttt tgggatcagc
      961 tcgccatgtg cttcatattc tgcagcgcct acgttaaata cttcgtagtt taaatctgct
     1021 cctgcagcgt ctaaaattct taaaacgctg tctgtgatct ctggtccgat tccatcgcct
     1081 ttaaatactg taattgtgtc tgccatctct atctctcctc cattgctgtg tattctttat
     1141 tatcacctac atacttggtt gcaggacgca tgatccttcc atcataaagc ttattttcaa
     1201 tgttatgagc aacccagccg accatacggc tgcacacaaa taatggtgtg taaagatctc
     1261 tcgggatacc taacatttca taagcaaaac cgctgtaata atctacatta gtaggaagtg
     1321 caactccctt gcgctctttc atagtctcta aggcaatacg ttcaaacctc tgacggaaat
     1381 caaattccgt catcatcttc ttttcctttg ctacttccaa gcagcgtgcc cttaacattt
     1441 ctgcacgagg atcggaaatg gtataaactg catggccaaa tccatataca agaccggtac
     1501 ggtcatataa ctcaccatcc atcagcttat taacagctgt tttgatcttc ttatcatctg
     1561 cggtatatcc gccggtttct tccagaaccg catccatcat ttccgcaacc ttgatatttg
     1621 caccgccgtg tctcggtccc ttcatggaac caatacttcc gcagacagtg gaatagatat
     1681 cggtgcctgt agaagagatc actacattgg taaatgtgga gttgttacca ccgccgtgat
     1741 ctgcatgaag catgagaatg gtatctaaca gtcttgcctc tttatcagag aacttctggt
     1801 cttttctcaa taaacttaag aagttttcag caatagagta tttcgggttt gcatagtgga
     1861 taaataagct gtctctgttg taataatgaa ctttgctctg atatgcatag caggcgatcg
     1921 acggaagctt tgccatcagg ttaatacctt ttaccagggt ttcatataca tccgtgttat
     1981 ccgggtcatc atcgtaattg tataaagtaa gaactgcatc ctgaagcttg ttcatcaggt
     2041 ttctgccagg catacgcaac aggttcattt ccacaaattc atccgggatc tcatagcagt
     2101 cagttaagat atttcggaaa tgctccagtt ccgctttctt tggcagatat ccaaagagaa
     2161 gaaggaagca gacttcctca taaccaaaat gactgtcccc tttacctttt accaggtctt
     2221 ctacatcata gcctctgtag taaagctttc ctggaatagc ctggatgcct ccctctggag
     2281 tttcgtcata gcccacaaca tctgccacac gggtaagtcc tactcttaca ccggtgccgt
     2341 cttcatttcg aagacctttc tttacattgt attctttata ccatttattt ggaatatccg
     2401 tataattctg ggattttcca taaaactgtg caagataact atctttcata acctgttctc
     2461 ctctcccggc atgttctcct actgctgtcg cttttgcgta tgatcaatgt ctgttccggt
     2521 tataattaat aaggcatccg gccttaacaa tggctctttc ttccggtgtc atatctgcaa
     2581 tataaagatt taactctttg atttctgcct ttgcatcttt tccgtttcca aggacatatg
     2641 ccttaatgga acttaaatcc ccatccaacg cttttctcac tcccggaacg tagatataat
     2701 cacctacttc aaagtcagga tctgcttcca tctggaatgg cagcatgccc cagttcataa
     2761 cattggagcg gtatctcttg gttgcatatt ccttacagat atttgcaagt ccgccgatga
     2821 ccctctggca gctggctgca tatt
//
LOCUS       DBMDUA010000280         2843 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_12112_length_2843_cov_2.364333, whole genome shotgun sequence.
ACCESSION   DBMDUA010000280 DBMDUA010000000
VERSION     DBMDUA010000280.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12112_length_2843_cov_2.364333"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="ACZPCL_13340"
     CDS             <1..599
                     /locus_tag="ACZPCL_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIR1163484.1"
                     /translation="FGVAIGMYMTDGLYYVSRVPGWYYILFIAEIGIVVYLSARINQM
                     TVGMARTMFFLYAAVNGIVFSVYFLVFDVVSLWMAFAITAAFFGVMALIGYFGNINFS
                     ALRPFMMAGLIFLAGFWLLSMFINLSAYETVVCAIGIFIFLIVTAYDAKKIQAFYSYY
                     GNVPEMATKSSIFAALQLYLDFINLFVYLLRFVGRRRR"
     gene            726..1607
                     /locus_tag="ACZPCL_13345"
     CDS             726..1607
                     /locus_tag="ACZPCL_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1163485.1"
                     /translation="MLIGALEAGGTKMVCALGREDGTIIEQVSIPTTTPEETIPKITA
                     YFKDKGIEALGIGAFGPVGVDPKADNYGYILETPKLAWRNKDLLGGLKKELNVPMGID
                     TDVNGSCLGEVTYGCAKGLDSVVYITIGTGVGVGVWVNGGLLHGMLHPEGGHILLNRH
                     PEDEKGGICPNHKNCLEGFASGPSIEARWGKKAIELKDDPKVWEIESYYIAQAITDYI
                     MILSPQKIILGGGVMHQEQLFTLIREKVKTMVAGYINTKELQDIDHYIVPASLHDDQG
                     IMGAIKLGLNALEQAKK"
     gene            1604..>2843
                     /locus_tag="ACZPCL_13350"
     CDS             1604..>2843
                     /locus_tag="ACZPCL_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1163486.1"
                     /translation="MSNSEPVSSLKGIGEKTGKLFEKLGVITIDDLLSYYPRAYDTYE
                     PPVPVGQLKEQMVMSVAAALPKPPDLLRTGKIQMVSAYVRDITGALQLAWYNMPYMRA
                     NIKSGQMYVFRGKVVKKRGRLMMEQPEVFTEEAYETVVNSMQPVYGQTRGLSNKTIVK
                     AEKQALENRQMKREYMPAFLRRKYELAEINYALEHIHFPRDQKELLFARKRLVFDEFF
                     MFLVGVRRLKESREGKKTEFVIRPGEETGRLKESLPYALTSAQERTLQEVFSDLQKGC
                     VMNRLVQGDVGSGKTVIAILTLLAAAENGYQGALMVPTEVLARQHFESITKMFETSGI
                     DKKVVLITGSMTAKEKRIAYEKTASHEADIIIGTHALIQEKVIYDKLALVITDEQHRF
                     GVGQRELLSEKGHTPHVLVMS"
BASE COUNT          825 a          532 c          725 g          761 t
ORIGIN      
        1 catttggtgt agcaattgga atgtatatga cagatggtct gtattatgta agccgggtac
       61 caggctggta ctacatcttg tttatcgcag agattggcat cgttgtatac ctgtcagcac
      121 ggatcaacca gatgacagta ggtatggcaa gaaccatgtt tttcctgtat gcagctgtaa
      181 acggtattgt attcagcgtt tatttccttg tatttgatgt agtatcttta tggatggcat
      241 ttgctattac tgctgcattt ttcggcgtta tggcgctgat cggatatttt ggaaatatca
      301 atttctctgc gctgcgtcca tttatgatgg caggactgat ctttttagca ggtttctggc
      361 tgttgtccat gtttatcaat ctgtctgctt atgagacagt tgtatgcgct atcggtattt
      421 tcattttcct gattgttact gcttatgatg caaagaagat ccaggcattt tacagctact
      481 atggaaatgt tccggaaatg gctacaaaat cttctatttt tgcagcatta cagctgtatc
      541 ttgattttat caacctgttt gtttatctac ttcgttttgt aggaagaaga cgtagatgat
      601 agtcattggc gaatgaaccg tctgcgttag cagacagtaa accacatttg tgggatattt
      661 gaatgcaaga gcccgtccgg taaggatggg ctgttgtaaa tatatgagat caaggggaga
      721 aaaatatgtt aataggagca ttagaagcag gcggcactaa aatggtctgc gcactgggaa
      781 gagaagacgg aacgatcatt gaacaggttt ccattccgac tactacgccg gaggagacta
      841 tcccaaagat cacagcatac ttcaaagata agggaattga agcgctggga attggagctt
      901 tcggacctgt gggagtagat ccgaaagcag ataattacgg ttatatcctt gaaacaccaa
      961 agctggcatg gagaaataag gatctccttg gcggtctgaa aaaagaactg aatgttccta
     1021 tggggattga tacagatgta aatggttcct gcctgggcga agtaacatat ggctgcgcta
     1081 agggactgga cagtgttgtt tatatcacca ttggaaccgg cgttggtgtt ggtgtatggg
     1141 taaacggagg acttcttcac ggtatgctcc atccggaagg tggacatatc ctgttaaacc
     1201 gtcatccgga agatgaaaaa ggcggcatct gcccgaacca taaaaactgc cttgagggct
     1261 ttgcaagcgg cccatcaatt gaagcacgat ggggcaagaa agccattgaa ttaaaagatg
     1321 atccaaaggt gtgggagatc gaaagctact atattgcaca ggcaattaca gattatatta
     1381 tgatcctttc accacagaag atcattttag gcggcggcgt tatgcatcag gaacagttat
     1441 ttacactgat ccgtgagaaa gtaaagacaa tggtagcagg atatatcaat acgaaagaat
     1501 tacaggatat agatcattat atcgttcctg caagtcttca tgatgatcag ggtatcatgg
     1561 gtgctatcaa attaggttta aatgcactgg agcaggcaaa gaaatgagta acagtgagcc
     1621 tgtaagttcc cttaaaggga ttggagagaa aaccggaaaa ttatttgaaa agctgggggt
     1681 catcactatt gatgaccttc tttcctatta tccaagagct tatgatacct atgagccgcc
     1741 tgtgcctgta ggccagttaa aagagcagat ggtcatgagc gtggcagccg cccttccaaa
     1801 gccgccggac cttctgcgta caggaaagat ccagatggtc tctgcctatg ttcgtgatat
     1861 aacaggtgcg ctgcagcttg cgtggtataa tatgccttat atgcgcgcca atatcaaaag
     1921 cgggcagatg tatgtattcc ggggaaaagt agtaaaaaaa cggggccgtc tgatgatgga
     1981 gcagccggaa gtctttacgg aagaagccta tgaaacagta gtaaattcca tgcagccggt
     2041 gtatggacag acaagaggct taagcaataa aaccattgta aaagcagaaa aacaggctct
     2101 ggaaaacaga cagatgaagc gggaatatat gcctgctttt ttacgcagaa aatatgagtt
     2161 ggcagagatt aattatgcct tagagcatat ccattttcca agagaccaga aggaactttt
     2221 atttgccaga aaacgtcttg tatttgacga gtttttcatg tttttagtag gagtgcgccg
     2281 gttaaaagag agcagggaag gaaagaaaac agagtttgtg atccggcccg gtgaagagac
     2341 aggacgctta aaggagtcgc tgccctatgc actgacctct gcccaggaac gtaccttgca
     2401 ggaagtattt tcagacctgc aaaaaggctg tgtgatgaac cgcttagtcc agggtgatgt
     2461 aggttccggt aagacggtta ttgccatcct gacacttctt gcagcagcgg aaaatggcta
     2521 ccagggcgct ttgatggtgc ctacggaggt gctggcaaga cagcatttcg agtccatcac
     2581 gaaaatgttt gaaaccagcg gtattgataa aaaagttgtc cttatcaccg gttccatgac
     2641 agccaaagaa aaacggatcg cctatgaaaa aactgcctcc catgaggcag atattattat
     2701 cggcacccat gcgctgatcc aggaaaaggt gatctatgat aaactggctc tggtgatcac
     2761 cgatgagcag caccggtttg gcgttggtca gagggaactt ttaagtgaaa agggacatac
     2821 acctcatgtt ctggtcatga gtg
//
LOCUS       DBMDUA010000281         2811 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_12282_length_2811_cov_1.860886, whole genome shotgun sequence.
ACCESSION   DBMDUA010000281 DBMDUA010000000
VERSION     DBMDUA010000281.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12282_length_2811_cov_1.860886"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="ACZPCL_13355"
     CDS             <1..511
                     /locus_tag="ACZPCL_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR1163487.1"
                     /translation="VLESLELFNKNARLFEAVISTLDVNKELLEKRAYESFSVVTDIA
                     DQMYRSYKIPFRRAHSFVSFLVKKAGKMEYNLRNISEEFFASSYEEFFDEPFKHDFTP
                     ISRCIDPWHFVRSREVRGGTGEKAMESMIERAKKEIEADQLWIHEHREQLHEADEKRK
                     QVIRNKLGS"
     gene            651..1817
                     /locus_tag="ACZPCL_13360"
     CDS             651..1817
                     /locus_tag="ACZPCL_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1163488.1"
                     /translation="MKKNNLSDVKKANRQLVYECIWSHKSVTMTDLAYVTHLSRPTIT
                     SLVQEMTADNLVIKSGYGTSNGGRNPVLYHANVNAAYAMGIDLEFPKVRMAISNLECE
                     NICFSVRIYPKDADKDQVLQLMKQQIDDLLLESGIDKNRLLGVGIGVPGVIDYKNNYS
                     VFFERINGWENVLIGDIIQEYIGKPVYICNDVNLISWAERKVAHLEDIKNMLYIIIRS
                     GIGMAIWNDGSLMQGEMGNSGRIGHMTVDKNGLQCKCGNRGCLGLYTSEKSMIRMYGE
                     LTGKELKWAGELFPLAEAGDAAALQVFETCGRYLGTGIVNVANLFDISEVVLSTSFDS
                     SYILKSAQYALDARKMNTIRREVKVRKGLLQESSFGLGGCLLVLNKEHMSLLEG"
     gene            1960..2541
                     /locus_tag="ACZPCL_13365"
     CDS             1960..2541
                     /locus_tag="ACZPCL_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIR1163489.1"
                     /translation="MTTKLTREQGLELLKKYNKEPFHILHGLTVEGTMRWFANELGYG
                     EDADFWAMAGLLHDIDFEMYPEQHCIKAPELLREAGAEEELIHAICSHGYGIACDVKP
                     EHEMEKVLFAVDELTGLVGAAARMRPSRSVMDMEVSSLKKKFKDKKFAAGCSRDVIRE
                     GAENLGWTLEELMEKTILAMRSCEESVNKEVEN"
BASE COUNT          916 a          431 c          703 g          761 t
ORIGIN      
        1 agtattagaa agtctggaac tgtttaataa aaatgcgcgg ttgtttgaag cagtgatcag
       61 tacattggat gttaataaag aactgctgga aaaacgggcc tatgaaagtt tttctgtagt
      121 aacagatatt gcagatcaga tgtatcgttc ttataagata ccatttagga gagcccattc
      181 atttgtatct ttccttgtta aaaaagcagg aaagatggag tataatttaa gaaatatttc
      241 ggaagagttc tttgcttctt cttatgaaga gttttttgat gaaccattta aacatgattt
      301 tacacctatt tccagatgta tcgatccatg gcattttgta agaagcaggg aagttcgtgg
      361 tggaactggc gaaaaagcaa tggagtctat gatcgagaga gctaagaagg aaatcgaagc
      421 tgatcagtta tggatccatg aacatagaga acagttacat gaagcggatg aaaagaggaa
      481 acaggtgatc cgtaataaac ttggtagttg atttttaaga gaatgagtta gggcagctgt
      541 tttatctttg gaaaagcagc tgccttttat tcattgacac tggtagttgg aatgagatat
      601 aatgaaaatg ggaaagcgta taaaaatgcg tgatctgtac aggaggggac atgaagaaaa
      661 ataatctgag cgatgtaaaa aaagccaaca ggcagttggt atatgaatgc atctggtccc
      721 ataaatcagt tacaatgact gacctggcat atgtgacaca cctgagccgt ccaacaataa
      781 ccagtctggt ccaggaaatg acagcggata acctggtgat caaaagtggc tatggcacgt
      841 ccaacggggg aagaaatccc gttctttatc atgcgaatgt aaatgctgca tatgctatgg
      901 gaattgacct tgaatttcca aaggtgcgga tggctatcag taatctggaa tgtgaaaata
      961 tctgtttttc tgtgaggata tatccaaaag atgcggataa ggaccaggta ttgcagctta
     1021 tgaagcagca gatagatgat ctgctccttg agtcgggaat tgataaaaat cgtctgctgg
     1081 gagtcgggat cggtgtccca ggagtgattg attataaaaa taattattct gtattttttg
     1141 aacggatcaa tggctgggag aatgttctga tcggggatat catacaggag tatataggca
     1201 agcctgtata tatatgcaat gacgtaaatc ttatttcctg ggcagaaaga aaagtggctc
     1261 atttggaaga tataaaaaat atgctttata tcattatccg ttcaggaatt ggtatggcga
     1321 tctggaacga tggaagcctt atgcaaggag aaatgggaaa ctcaggtcgt attggtcata
     1381 tgacggttga taaaaatggt ctgcagtgca aatgcggtaa cagaggctgt ctgggccttt
     1441 atacaagcga aaagtccatg atccggatgt atggggaact tactggaaaa gaacttaaat
     1501 gggcgggaga attgtttcct ctggcagaag caggagatgc agcggccctg caggtatttg
     1561 agacctgtgg aaggtatctg ggaacaggaa ttgtaaatgt agccaatctc tttgatatat
     1621 cagaggtagt actttcaact tcatttgatt cttcttatat ccttaaaagt gcccagtatg
     1681 cactggatgc aagaaagatg aatacgatcc gtcgggaagt aaaggtgaga aaaggacttt
     1741 tgcaagaaag cagttttggt ctgggcggat gcctgttggt tttaaataaa gaacatatga
     1801 gtcttctgga agggtgatct cttccaggag attttctttt tgaacagatt tttctgccct
     1861 tgccttttgt attttacagt actataataa tgagaaataa taaaaagata tattcctaca
     1921 ggatatatct gaatatccgg tagaaaggaa gcagaagaga tgactacgaa acttacaaga
     1981 gaacagggac tggaattact gaaaaagtat aataaggagc catttcatat tttacatggt
     2041 cttacagtag agggaaccat gagatggttt gcaaatgaac tgggatacgg tgaagatgcg
     2101 gatttctggg ctatggcagg acttcttcac gatattgatt ttgaaatgta tccggaacag
     2161 cactgtatca aggcgccaga gctgttaaga gaagcaggag cagaagaaga gctgatccac
     2221 gccatctgca gccatggtta cggcattgcc tgtgatgtaa agccggaaca tgagatggaa
     2281 aaggtgcttt ttgcagtaga tgagcttaca ggtcttgtag gagcagcagc cagaatgcgt
     2341 ccttcccgca gcgtaatgga tatggaagtt tccagcctta aaaagaaatt caaagataaa
     2401 aagtttgctg ccggatgttc cagggatgtg atccgtgaag gtgcggaaaa cctgggctgg
     2461 acactggaag aactgatgga aaagaccatt cttgccatgc gttcctgtga agagtctgta
     2521 aataaggaag tggaaaatta aaatgctgcg ttaacaggct aagagcagaa gcttaaataa
     2581 aacgttaaat aaaagacaga accattttaa agtatgccgt gcaaagacat ttgtccttgc
     2641 acggcaaatt ttttagtcca aaactattga catgatggga aaagtacggt atcatttgca
     2701 cttgtaacgc catacatata aagtggcatt tattttatga aatacgctcc atatccggcg
     2761 catagaaaga aacaagggag acagacaaga tgaaaaaagt ggtgaagttc g
//
LOCUS       DBMDUA010000282         2734 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_12741_length_2734_cov_1.944550, whole genome shotgun sequence.
ACCESSION   DBMDUA010000282 DBMDUA010000000
VERSION     DBMDUA010000282.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12741_length_2734_cov_1.944550"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /gene="yihA"
                     /locus_tag="ACZPCL_13370"
     CDS             complement(<1..447)
                     /gene="yihA"
                     /locus_tag="ACZPCL_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR1163490.1"
                     /translation="MIIRDVNLETVCGVTSKLPDNTLPEFAFAGKSNVGKSSLINALM
                     NRKAYARTSSQPGKTQTINFYNINKELYYVDLPGYGYAKVSLEAKEKWGKMIERYLRN
                     SAMLKLVFLLIDIRHEPSANDKMMYDWIIYNGDHEPDQNKRGDHPAS"
     gene            complement(444..>2734)
                     /gene="lon"
                     /locus_tag="ACZPCL_13375"
     CDS             complement(444..>2734)
                     /gene="lon"
                     /locus_tag="ACZPCL_13375"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR1163491.1"
                     /translation="PTVALRGMTILPGMVQHFDVSRPKSIAAVEKAMLGDQKLFLVSQ
                     KRPEIPDPELSDLYQIGTVSVVKQLVKLPSKNVRVLVEGLEKAELLCFDTEEPYLMGE
                     ISLMGEEEEDTEPLAGEAMLRILKDKLEEYGTVNQRVAKETLPALLLIGDLKEMLKQI
                     PIQMPWDYTVCQAILECSSISAKYEAVLQTLMTEMEVYRIKKEFQEKVKADIDQNQKE
                     YILREQMKIIRQELGEDNPLSDADEYQKKTESLKADKEVKEKLLKEIGRFRTMPAGSQ
                     EANVLRTYIETLLELPWKKTSKDNEDIRHAEKILNEDHYGLEKVKERILEYLAVRSLT
                     KKGTSPIICLVGPPGTGKTSIARSVAKALNKKYVRISLGGVRDEAEIRGHRKTYVGAM
                     PGRLVEGLRQAGVANPLMLLDEIDKVSSDYKGDTSSALLEVLDGEQNVKFRDHYVEVP
                     VDLSQVLFIATANTTQTIPGPLLDRMELIEVNSYTENEKFHIAKDYLVGKQIERNGLT
                     PQQIVFSDKCLEKIIHNYTREAGVRNLERRIGDVCRKAARKYLEKKQGPIRVNEGNLE
                     KYLGKERVSFEDANEEDEVGIVRGLAWTSVGGDTLQIEVNVMPGKGNLLMTGQMGDVM
                     KESAQIALTYVRSICPDYQVADDYFEKHDLHLHIPEGAVPKDGPSAGITMATAMLSAV
                     IGRKVLAKVAMTGEITLRGRVLPIGGLKEKILAAKMAHIKKVLVPDKNRPDMAELSKE
                     ITRGLEIVYVKNMDEVIKEAFV"
BASE COUNT          627 a          708 c          543 g          856 t
ORIGIN      
        1 ggaggccgga tgatcgccgc gtttattctg gtctggctcg tgatcgccgt tatagatgat
       61 ccagtcatac atcatcttgt cgttggcaga tggttcatga cggatatcga tcagaagaaa
      121 aaccagtttt aacatggcgg agtttctaag ataacgctcg atcatcttgc cccatttttc
      181 cttggcttcc agtgaaacct tggcataacc atatccagga agatccacgt agtacaactc
      241 tttattgata ttgtaaaagt taatggtctg ggtctttcca ggctgggaag aagttcttgc
      301 gtaggctttc cggttcatca gtgcattgat caatgaggat tttcctacat ttgactttcc
      361 tgcaaaagcg aactccggca gtgtgttgtc cggaagtttg ctggtaacac cgcagactgt
      421 ttccagattt acatctctga tgatcataca aatgcctcct ttattacttc atccatattt
      481 ttaacataaa cgatctccaa acctctggtg atctcttttg ataattctgc catatccggc
      541 cggttcttat ccggaaccag gactttttta atgtgggcca tcttggctgc caggatcttc
      601 tccttcaggc cgccgatcgg aagcactctt cctcttaagg taatctcacc ggtcatagcc
      661 acttttgcca gaactttccg tcctattaca gcagaaagca tagctgttgc catggtaata
      721 cctgcagacg ggccatcctt tggaactgca ccctccggaa tatgcagatg cagatcatgt
      781 ttttcaaagt aatcatctgc cacctggtaa tccggacaga tcgagcgcac ataagtcaga
      841 gcaatctgtg ctgactcctt cattacatca cccatctggc cagtcataag gagatttcct
      901 tttccaggca tcacatttac ttcgatctgt aaggtatcgc cgcctacgct ggtccatgcc
      961 agacctctta caatacctac ttcatcttct tcattggcat cttcaaaaga gactctctct
     1021 tttccaagat atttttccag atttccttca tttacacgga tcggtccctg cttcttttcc
     1081 agatattttc tggctgcctt gcggcacaca tccccgatcc ggcgctctaa gtttcgcaca
     1141 ccggcttctc tggtgtaatt gtggatgatc ttttccagac acttatcgga aaatacgatc
     1201 tgctgtggtg taaggccatt tcgctcgatc tgttttccta ccagatagtc ctttgcaatg
     1261 tggaacttct cattttctgt atagctgttt acctcgatca gttccatacg gtcaagtaat
     1321 ggtcccggta tggtctgggt ggtattggca gtggcaataa acagtacctg ggaaagatcc
     1381 acaggaactt ctacatagtg atcgcggaat tttacattct gctccccgtc taatacttcc
     1441 agcaaagcag aggaagtatc tcccttgtag tcactgctta ccttatcgat ctcatctaaa
     1501 agcatcagcg gattggcaac tcctgcctgg cgcagtcctt ctaccagacg tcccggcatt
     1561 gcccctacat aagtctttct gtgtccacgg atctctgcct catctcttac accgccaagg
     1621 ctgatgcgca catatttttt atttaatgct tttgcaacag atcttgcaat ggaggtctta
     1681 ccggttcctg gcggacctac taaacagatg atcgggcttg tacccttctt ggtaagagaa
     1741 cgcacagcca gatactctaa aatacgctct tttacctttt ccagaccata atgatcctca
     1801 tttaagatct tttctgcatg gcggatatct tcgttatctt tggaagtttt cttccacggc
     1861 agttctaaaa gagtctctat gtaggtccgc agcacattgg cttcttggct tccggcaggc
     1921 atggtacgga aacggccgat ctcttttaac agcttttcct ttacttcttt gtctgctttt
     1981 aagctttctg ttttcttctg atactcatct gcatcagata aaggattatc ctcccctaac
     2041 tcctgacgga tgatcttcat ctgttctctg aggatatact ctttctggtt ctggtcaata
     2101 tctgctttta ctttctcctg gaattccttt tttatacggt aaacttccat ttctgtcata
     2161 agggtctgaa ggacagcctc atattttgca gatatgctgg aacactctaa gatcgcctgg
     2221 catacggtat aatcccatgg catctggata ggaatctgct ttaacatctc cttcagatca
     2281 ccgatcagca gcagcgccgg aagtgtttcc tttgccactc tctgattgac agttccatat
     2341 tcttccagtt tatcctttaa aatacggagc attgcctctc cggcaagagg ttctgtatct
     2401 tcttcctctt cccccatgag gctgatctca cccataagat aaggttcttc cgtatcaaag
     2461 cagagaagtt ccgctttttc cagaccttcc actagaacgc ggacgttttt agagggaagc
     2521 tttaccagct gttttactac agaaacggta ccgatctgat acagatccga aagttccgga
     2581 tccggtatct ccggacgctt ctggctgact aaaaacagct tctgatcccc taacattgcc
     2641 ttttcaaccg ctgctatgga tttcggtctg cttacatcaa aatgctgcac catacccggc
     2701 aggatggtca ttccccgcag ggccacagtg ggca
//
LOCUS       DBMDUA010000283         2691 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_12976_length_2691_cov_1.654440, whole genome shotgun sequence.
ACCESSION   DBMDUA010000283 DBMDUA010000000
VERSION     DBMDUA010000283.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12976_length_2691_cov_1.654440"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="ACZPCL_13380"
     CDS             <1..167
                     /locus_tag="ACZPCL_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HIR1163492.1"
                     /translation="VIDEFDGVLPFNDKARPETILREFHMSKNAFKRAVGHLLKEGKI
                     RITEKTIERV"
     gene            281..1129
                     /locus_tag="ACZPCL_13385"
     CDS             281..1129
                     /locus_tag="ACZPCL_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1163493.1"
                     /translation="MQKIWKDYGAITIGTILIGLATKNIFDPASMVTGGVSGIAIIGK
                     ELWNLPLWVTNTVLNIPLFLAGFKIMGWKFIKRTLYATVLLSVAFYILPEGMYIEDDL
                     LLSALFGGIITGVGTGLVLAGGCTTGGTDMLAALIRAKFPHYSVAQIMQVLDGLVVVA
                     GATVFGIRAALYALIAIFCMGKVADGLIEGMKFSKQVYIISDKYKEISDAIMTRMGRG
                     VTGLAAKGMYSDETKNMLFCVVSRKEIVEIKEIVSEYDPKAFFIVSDAREVFGEGFIE
                     DKNQLT"
     gene            1357..2469
                     /locus_tag="ACZPCL_13390"
     CDS             1357..2469
                     /locus_tag="ACZPCL_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1163494.1"
                     /translation="MASLSLKNVTKKYPNGFVAVKDFNLEIADKEFIIFVGPSGCGKS
                     TTLRMIAGLEDISSGELYIDGKLVNDVEPKDRDIAMVFQNYALYPHMSVYDNMAFGLK
                     LRKTPKDEIDKKVHDAAKILDLEHLLDRKPKALSGGQRQRVAMGRAIVRSPKVFLMDE
                     PLSNLDAKLRGQMRVEISKLHQRLETTIIYVTHDQTEAMTLGTRIVVMKDGVVQQVDS
                     PQNLYDNPCNKFVAGFIGAPQMNMIDAQVAKNGSDVTLAFGGHTLTLPEAKGKALEAA
                     GYVGKVVTLGIRPEDLHDEEAFLAANPKSTFQVEIRVYEMLGSEVLLYFDIDEANFTA
                     KVNPRTTARPGDTVKLAMDLDKVHIFDKETECVIIN"
BASE COUNT          857 a          486 c          622 g          726 t
ORIGIN      
        1 aggtgatcga tgaatttgac ggagttcttc cttttaatga taaggcaaga ccggagacca
       61 ttttaagaga attccatatg agcaagaacg catttaagag agccgttgga catctcttaa
      121 aagaaggcaa gatacggatt acggaaaaga ccatagaaag ggtataagaa gtactcttaa
      181 aatattgcag gagaatatcc gcagatataa atatgggtag cagaaagaca gttgtgttta
      241 gcagctgtct tttgttttta gataaggagg aaacataaaa atgcagaaaa tatggaaaga
      301 ttacggtgcc atcaccatag ggaccatcct cattggtctg gccaccaaaa atatttttga
      361 tcccgccagc atggtaacag gaggcgtttc cggtattgcc attattggta aggaactttg
      421 gaacctgcct ttatgggtca ccaatacggt tttaaatatc ccgctgtttt tggcaggttt
      481 taagatcatg ggatggaaat ttattaaacg tacattgtat gcaacagtgt tactgtccgt
      541 tgccttttat atcctgccgg aaggtatgta tatagaagat gatctgctgc tgtcggctct
      601 ttttggcggt atcatcacag gcgttggtac aggtcttgta ctggccggag gctgcactac
      661 agggggaaca gatatgctgg ccgctctgat ccgggcaaaa ttcccccatt attccgttgc
      721 gcagatcatg caggtattag acgggctggt agtagtagcc ggtgcaaccg tatttgggat
      781 ccgcgctgcc ctttatgcac tgatcgctat tttctgtatg ggaaaagttg cagatggtct
      841 tattgaagga atgaaatttt ccaagcaggt ttatattatt tcagataaat ataaagaaat
      901 ttcagatgcc atcatgaccc gtatgggaag aggcgttaca ggtcttgcgg caaaaggaat
      961 gtattcagat gagacgaaaa atatgctttt ttgtgtagtt tccagaaaag agatcgtgga
     1021 gatcaaagaa attgtatcag aatacgatcc gaaagcattt tttatcgtca gtgacgcccg
     1081 tgaagttttt ggtgagggat ttattgaaga taaaaaccaa ttaacataat aaccaaagtt
     1141 tttgaggcaa acgaaggaaa actttgacag atacagcact tttttaattg aatatacatt
     1201 ttagctaaat tccaaattta gattttgtgg atttacagta tggaataaaa atacgctttc
     1261 ctgtatgata gagacagttg caaaagacct atatgaactt cctaaaaagc ctgtaaaagg
     1321 gcaggaagta catctataaa tcaggaggaa aatagaatgg caagtttatc actgaagaat
     1381 gtaaccaaga aatatcctaa cggatttgtg gctgttaagg acttcaatct ggagatcgct
     1441 gacaaggaat tcatcatctt cgtaggccct tcaggatgcg gaaagtcaac cactcttcgt
     1501 atgatcgcag gtctggaaga tatttcatcc ggtgaactgt atatcgatgg aaaactggta
     1561 aatgacgtag agccaaaaga cagagatatt gcaatggtat tccagaacta cgctctgtat
     1621 ccacatatgt ccgtatatga caacatggca ttcggcctga agttaagaaa gacaccaaag
     1681 gatgagatcg ataagaaagt acatgacgca gctaagatcc tggatctgga gcatctgtta
     1741 gaccgtaagc caaaggcttt atccggtggt cagagacagc gagttgctat gggacgtgcc
     1801 attgtccgca gccctaaagt attcctgatg gatgagcctc tgtcaaacct ggatgcaaag
     1861 ttaagaggcc agatgcgtgt tgagatctcc aaattacatc agagactgga aacaaccatc
     1921 atctacgtta cacatgacca gaccgaggct atgacactgg gtaccaggat cgttgttatg
     1981 aaggatggtg ttgtacagca ggtagacagc ccacagaact tatatgacaa tccatgcaac
     2041 aagttcgttg caggatttat cggcgcacct cagatgaaca tgatcgatgc ccaggtagct
     2101 aagaacggtt ctgatgtaac tttagcattc ggcggacata cattaactct gccagaagca
     2161 aagggcaaag cactggaagc agctggttat gtaggcaagg tagttaccct tggtatccgt
     2221 ccagaagatc tgcatgacga agaagcattc ctggcagcta atccaaagag taccttccag
     2281 gtagagatcc gtgtatacga aatgttaggt tctgaagttc ttctgtactt tgatattgac
     2341 gaagctaact tcactgcaaa ggttaaccca cgtaccactg caagacctgg cgataccgta
     2401 aaactggcta tggatctgga taaggttcac atctttgata aagaaacaga atgcgttatc
     2461 attaactaat tattagctaa tattcagagg aaatagtgaa aatcctctgt agaaaacgac
     2521 agtaaaaaaa ggcaggagag tttacaactc tcttgctttt tttgttattt tgcattaaaa
     2581 tatgaaaata gggaaaacag taactccacc atgatttatc atggcaatgt tcataaattt
     2641 tcaataaggc gaaaaggaga gagttgaatg atctcaaatc agatacttca g
//
LOCUS       DBMDUA010000284         2687 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_12991_length_2687_cov_2.594741, whole genome shotgun sequence.
ACCESSION   DBMDUA010000284 DBMDUA010000000
VERSION     DBMDUA010000284.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12991_length_2687_cov_2.594741"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="ACZPCL_13395"
     CDS             complement(<1..508)
                     /locus_tag="ACZPCL_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wadjet anti-phage system protein JetA family
                     protein"
                     /protein_id="HIR1163495.1"
                     /translation="MHEIPERFWSLFRSVNRATYIEALLKINEEYEYSNYFLSREMCI
                     QLLSSYFAQKRYVIWQDELEEEEDQLEPPATRVLNWLLKTGWLRKVDDYSTMTVNIVI
                     PDYAAVMIEAFHRLSNEQEDETQIYIQNVYAILFSLKNDSRAGIGLLDTAIINTRKLN
                     KSLQDLLHN"
     gene            complement(530..1237)
                     /locus_tag="ACZPCL_13400"
     CDS             complement(530..1237)
                     /locus_tag="ACZPCL_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004069843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR1163496.1"
                     /translation="MAFLSDLRRFYGTGEKNPAGQSLEEFLEHYNPAKYESLCNTADI
                     VVVRCEEKLTHWGQPLKVLMVKRSNHPSIGFWATPGGFVELKEDISEGAARELEEETC
                     VKGLPLRQMRTWGEWQRDPRWRIITTSYLALVEGDLKVQAADDAKDAQWLDVDLKRLS
                     TEEKEDQSSVEVWELSLTNKEQGIDVSAKVEITYSGHPLIKEETYHLLESKGIAVDHG
                     CIVTNALLYLKNQLEGK"
     gene            complement(1270..>2687)
                     /gene="cobA"
                     /locus_tag="ACZPCL_13405"
     CDS             complement(1270..>2687)
                     /gene="cobA"
                     /locus_tag="ACZPCL_13405"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF014641.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR1163497.1"
                     /translation="KEFLSWVPSRCKTFYVGKQAGCHSMKQDQINELLVQLALEGNKV
                     VRLKGGDSFVFGRGSEEILALKEKEIPWELVPGITSCVSVPELAGIPVTHRNVSRSFH
                     VITGHTLKGAQSEAELALYGACEGTLVFLMGLSNLEHIAKGLIKGGKSPDTPAAVIED
                     GSLASQRTVRGTLETIYEKSLKAGLKTPAIIVVGETAAFDLRSEEMTGDKIDDKPLSG
                     LTVGITGTSSFTARLETALKSQGAKTLPVMKMEVVPEAFSEEFSEDPDSSNPNSSLTA
                     ILKQNFTWLVFTSVNGVEIFFDRFLNQENGDLRLLCSLHFAVIGEATRQALKAHGFQA
                     DLMPKEYCSQSLAEALINVLTPSDHVLLARSKDGSVNLSKELEKQDIFYKDLALYHIK
                     GKPLADGTLLSQCSHLVFGSASGAKAFLKHFQIPAGVKVIAIGPVTGKALREAGVIPY
                     MTGNSYDIPGILKGLTEEKTR"
BASE COUNT          667 a          674 c          568 g          778 t
ORIGIN      
        1 tattgtggag caggtcctga agggatttgt ttaatttccg cgtattgatg atcgctgtat
       61 ccaacaggcc gatcccggca cggctgtcat ttttcaggga aaacaggatg gcatatacgt
      121 tctggatata gatctgggtc tcatcttcct gttcattgga aagacggtga aaggcctcga
      181 tcatcacagc cgcgtagtcc ggaatgacaa tgttcacggt catggtggaa taatcatcta
      241 ctttccgcag ccaccctgtt tttaacagcc agtttaacac acgggttgcc ggaggttcta
      301 actggtcctc ctcttcctcc agttcatcct gccagatcac ataccgcttc tgggcaaagt
      361 aactgcttaa aagctggatg cacatttctc ggctgagaaa gtaattgctg tattcatatt
      421 cctcattgat ctttaaaagt gcttctatgt aggtggcgcg gttgacggaa cggaacaggc
      481 tccagaaacg ttccggtatc tcatgcatta aatttgtttt caatctgtat tacttccctt
      541 ccagctggtt tttcagataa agaagcgcat tggtaacgat gcagccatgg tcaacggcaa
      601 tgccctttga ctctaataag tgataggtct cttccttgat cagaggatga ccggaataag
      661 tgatctccac ctttgcagaa acatcaatgc cctgctcctt attggttaaa gacagctccc
      721 acacctctac agatgactgg tcttcctttt cctctgtgct taaacgtttc agatccacat
      781 ccagccactg ggcatctttt gcgtcatctg cagcctgtac tttcagatct ccttctacca
      841 aagccagata ggaggtagta atgatacgcc atcttggatc gcgctgccat tctccccagg
      901 tacgcatctg gcgcagtggc agtcccttta cacaggtctc ttcttccagt tctctggcag
      961 caccttcact gatgtcctct ttcaactcta caaagcctcc tggagtcgcc cagaaaccga
     1021 tacttggatg attgctgcgc tttaccataa gaacctttaa tggctgtccc cagtgggtca
     1081 gtttttcttc acatctgact actacaatat cagctgtgtt gcacaggctt tcatacttgg
     1141 ccggattata atgctctaaa aactcctcta aagactgtcc agccgggttt ttctcacctg
     1201 taccataaaa tctccttaaa tcacttaaaa atgccataaa aatgccctcc ttcttgtact
     1261 gctgtacatt tatcgcgttt tttcttcggt cagccccttt aatatgccag ggatatcata
     1321 agaatttccg gtcatatacg ggatcactcc ggcttcacgt aaggctttgc cggttaccgg
     1381 gccaatggcg atcactttga cacctgcagg tatctggaaa tgctttaaaa atgctttcgc
     1441 gcctgaggca ctgccaaaga ccaggtggct gcactgtgaa aggagtgttc cgtcagcaag
     1501 aggcttccct tttatatgat acaacgccag gtctttataa aaaatatctt gtttttccaa
     1561 ttctttgctc aaatttacac tgccgtcttt ggaacgtgcc aaaagcacat gatctgaagg
     1621 agtcagaaca ttaataagag cttctgccag gctttggctg cagtactctt ttggcatcag
     1681 atccgcctga aagccatggg cttttaaagc ctgtctggtg gcttctccga tcacggcaaa
     1741 atgaagactg cataaaagcc gcagatcccc gttttcctgg tttaaaaacc ggtcaaagaa
     1801 aatttccact ccgtttacac tggtaaagac cagccaggtg aagttttgtt ttaaaatagc
     1861 tgtaagtgaa gagttcggat ttgaagagtc tggatcttct gaaaactctt ctgaaaatgc
     1921 ctccggcacc acttccattt tcattacagg aagtgttttg gcgccctggg attttaaagc
     1981 agtttctaaa cgggctgtaa aagaagaggt accggtgatc ccgactgtaa gcccggataa
     2041 tggcttgtca tcaatcttat cacctgtcat ttcttctgat cgcaggtcaa aagctgcggt
     2101 ctcccctaca acaatgatcg caggtgtttt aagacctgcc ttaaggcttt tctcataaat
     2161 ggtttccagt gtacctctga ctgtgcgctg actggcaaga gagccatctt cgatcacagc
     2221 tgccggtgta tccgggcttt ttcccccttt tataagacct tttgcaatat gctccagatt
     2281 ggaaagtccc ataagaaaga ccaaagttcc ctcacaggca ccatataaag ccagctctgc
     2341 ctctgactgg gcccccttta aggtgtgtcc ggtgatcaca tgaaagctgc ggcttacatt
     2401 tctgtgtgtg accggtatac cagccagttc cggaacagat acgcaggaag taatacccgg
     2461 caccagctcc caggggatct ctttttcttt taaagccagg atctcttctg aaccacggcc
     2521 gaaaacaaag gagtccccgc cttttaagcg aactacttta tttccttcta aagccagctg
     2581 taccagaagt tcattgatct gatcctgttt catagagtgg cagcctgcct gttttcctac
     2641 ataaaaagtt ttacaacggg aaggaaccca ggagagaaat tcttttg
//
LOCUS       DBMDUA010000285         2687 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_12992_length_2687_cov_2.264501, whole genome shotgun sequence.
ACCESSION   DBMDUA010000285 DBMDUA010000000
VERSION     DBMDUA010000285.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12992_length_2687_cov_2.264501"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="ACZPCL_13410"
     CDS             <1..474
                     /locus_tag="ACZPCL_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1163498.1"
                     /translation="LIIIPIYLMMSKMGLINTYRGLVLCYIAFELPICIWTMQSYFET
                     IPSDLIEAAEIDGLAKVQTLWHIFLPVALPGLAASGIMTFIGIWNNFLLANTLLIDEA
                     KKTLPVVIADFSSRDSMLQGDVLAASMIVCIPSFLFALFAQKYLVGGLTSGSVKG"
     gene            487..1629
                     /gene="dgoD"
                     /locus_tag="ACZPCL_13415"
     CDS             487..1629
                     /gene="dgoD"
                     /locus_tag="ACZPCL_13415"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HIR1163499.1"
                     /translation="MKITDVRTHLIDQYMFVQVDTDEGISGVGESGAWAFLEASKEAV
                     ETFKRYLIGQDPLLIEHHWQYMYRCYHFRGAAIMGALSAIDIALWDIKGRYYGVPCYE
                     LLGGKVRHKARVYYHVFGSSGEELIQGCKEAKARGFKAVGHLTPFVDSDRREVMKMES
                     FATKISNAAERVYQYRETIGNEVDLCIEIHRQLNVPEAIALGREIEKARPMFYEDPVR
                     PDNFDDMQRVASEIHIPIATGERLHTLEEFGMLLARNACQYIRPDVCLCGGITQAKKI
                     AAAAEARGVLVVPHNPLSPVSTAACIQLAASIPNFAIQEYPLGEDCAPKNEIVKSTLT
                     LDNGYLTVPDSPGIGIELNEQAMHQYPYKPRLYVTKLREDGSVVDQ"
     gene            1675..>2687
                     /locus_tag="ACZPCL_13420"
     CDS             1675..>2687
                     /locus_tag="ACZPCL_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR1163500.1"
                     /translation="MKLPMTYEELRKQMLDEEGAKYPGKALVNMELEPGYNRAKNRML
                     PYIQMINEAHLIMLIEQGIVTEEDGKIILKALEEVDQEFYKNTVYDGKYEDLYFFMES
                     RLIEATDGVAGNLHLARSRNDMCVAWSHLAVRDGLLKAMEQLLKLKNAILLFAEEHKN
                     TLYVIHTHTQHAQPGLLGHYFLGAADMLERDFKRLCMAYEAVNLSPMGAAAVTTTGFP
                     ISRERVAELSGFSGIIENAYDAIGNSDYLTQTASALGLCALDLGRVVTDMVLWATQEM
                     NMIHVADGYISISSIMPQKRNPIALEHLRSSLSVLKGMTEMVLTGFLKSPYGDISDYE
                     DIE"
BASE COUNT          814 a          482 c          687 g          704 t
ORIGIN      
        1 ctaatcatca tacctattta tcttatgatg tcaaaaatgg gactgatcaa tacatacaga
       61 ggactggttt tgtgttacat agcctttgaa cttcccattt gtatatggac catgcagtct
      121 tattttgaaa caattccatc tgatctgatc gaggcagcag agatcgatgg cctggcgaaa
      181 gtacagactc tgtggcacat tttccttcct gtagcgttac cgggacttgc agcaagcggc
      241 attatgacat ttatcggtat ctggaataac tttctgttag ccaatacctt actgattgat
      301 gaagccaaga aaacattacc tgtagtaatt gcggatttca gttccagaga cagtatgcta
      361 cagggggatg tgctggcagc ctctatgatc gtgtgtatcc cgtcatttct gtttgcgtta
      421 tttgcgcaga aatacctggt gggaggactt accagcggtt ctgtaaaggg ctagaaagga
      481 gtaaggatga agataacaga tgtaaggacc catttgatcg atcagtatat gtttgtgcag
      541 gtggatacag atgaaggaat atcaggcgta ggagagtccg gtgcctgggc ttttttagag
      601 gcatcaaaag aggcagtgga aacttttaaa aggtatctga tcggccagga tcctcttttg
      661 atagagcatc actggcagta tatgtaccgg tgttatcatt tccggggagc tgctattatg
      721 ggtgcactgt ctgccattga tatagcgtta tgggatatca agggaagata ttatggagtg
      781 ccctgttatg aactattagg aggaaaagta cgccataaag cacgggttta ttatcatgtg
      841 tttggttctt ccggagaaga attgatccag gggtgcaaag aagcgaaagc cagagggttt
      901 aaggcagttg gtcatctgac tccttttgta gactctgacc gaagagaagt tatgaagatg
      961 gaaagttttg ccacaaaaat aagcaatgcg gcagagcgtg tttatcagta cagagaaacg
     1021 attggaaatg aagtggatct ttgtattgaa atacacaggc aattaaatgt accggaagcg
     1081 atcgcactgg gaagagaaat tgaaaaagcc agaccgatgt tttatgaaga tcctgtaaga
     1141 ccggataatt ttgatgacat gcaaagagtg gcaagtgaga tacatattcc aattgctaca
     1201 ggagaaaggc ttcatacact ggaagaattt ggaatgcttc ttgcacgcaa tgcatgccag
     1261 tatatccgtc cggatgtgtg cctgtgcgga ggaattacgc aggcgaaaaa gatagcagct
     1321 gctgcggaag caagaggtgt actggtcgtg cctcacaatc cattaagtcc ggtgagcacg
     1381 gcagcctgca tccagctggc ggcttccata ccgaattttg ccatccagga atatccatta
     1441 ggagaggact gtgcgcctaa gaatgagatc gtaaaatcca cacttacatt ggataatggt
     1501 tatctgactg tgccggatag cccgggaata gggatcgaac tgaatgaaca ggcaatgcat
     1561 caatatcctt acaaaccacg tttatatgtg accaagttaa gagaagacgg gtcagttgta
     1621 gaccagtagt ccagactgtg atttgaaatg acaaataaag gagggaactg tgatatgaaa
     1681 cttccaatga cctatgaaga attaagaaaa cagatgttag atgaagaagg agcgaaatat
     1741 cctggaaaag cactggtgaa catggaactg gagccaggat ataacagggc aaaaaacaga
     1801 atgcttccct atatccagat gatcaatgaa gcccatctga tcatgttgat cgaacagggg
     1861 atcgtaacag aagaagatgg aaagatcatt ctgaaagcat tagaagaagt agatcaggaa
     1921 ttttacaaaa atacggtgta tgacggcaag tatgaagacc tgtatttctt tatggagagc
     1981 aggctgatcg aggcaacaga tggtgttgcg ggaaatctgc atctggcaag aagccgtaat
     2041 gatatgtgtg ttgcctggtc ccatctggca gtaagagatg gacttttaaa ggcaatggag
     2101 cagttgctga agttgaaaaa tgcgatatta ctgtttgcgg aggagcataa aaatactctg
     2161 tatgtgatcc atacccatac ccagcatgcc cagccgggat tattaggaca ttacttcctt
     2221 ggtgctgcag atatgctgga acgtgatttt aaacggcttt gtatggcata tgaagcagta
     2281 aatctgtcac cgatgggagc agctgccgtg acaactaccg gttttccaat ttccagggaa
     2341 agagtggcag agttatccgg attttccggt attatcgaaa atgcttatga cgccattggc
     2401 aacagcgact acctgacaca gacagcaagc gcactgggac tttgcgcctt ggatttggga
     2461 agagttgtca cagatatggt attgtgggcg acacaggaaa tgaatatgat ccatgtggca
     2521 gacggatata tttccatcag ttctattatg ccccaaaaaa gaaatccaat cgcattagaa
     2581 catttaagat cctctctttc tgttttaaag ggaatgacag aaatggtact gaccggcttt
     2641 ttgaagagtc cttatggtga tatttctgac tatgaagata ttgagga
//
LOCUS       DBMDUA010000286         2665 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_13112_length_2665_cov_1.693838, whole genome shotgun sequence.
ACCESSION   DBMDUA010000286 DBMDUA010000000
VERSION     DBMDUA010000286.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13112_length_2665_cov_1.693838"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /locus_tag="ACZPCL_13425"
     CDS             <1..99
                     /locus_tag="ACZPCL_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large ribosomal subunit protein bL28"
                     /protein_id="HIR1163501.1"
                     /translation="ANVKSVRVKVNGGTQRMYVCTSCLRSGLVERA"
     gene            complement(208..345)
                     /locus_tag="ACZPCL_13430"
     CDS             complement(208..345)
                     /locus_tag="ACZPCL_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163502.1"
                     /translation="MKRVWGLMLFCFGLGMAVLFFVPETIFTFFFVIGCLILGYNLFC
                     C"
     gene            complement(408..818)
                     /locus_tag="ACZPCL_13435"
     CDS             complement(408..818)
                     /locus_tag="ACZPCL_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HIR1163503.1"
                     /translation="MAENHFTSTIESLFKGMDQFVTTKTVVGEPVQVGETIIIPLIDV
                     TCGMAAGAFAEGSKQKDKGCGGMSAKMSPSAVLVIQNGVTKLVNVKNQDAVTKVIDMA
                     PDLINRFLGGKPAVSEETVGIAKETAASYEGKKA"
     gene            complement(871..1929)
                     /locus_tag="ACZPCL_13440"
     CDS             complement(871..1929)
                     /locus_tag="ACZPCL_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HIR1163504.1"
                     /translation="MHIVLLILKILGIILLVLFGLLLLVLCTVLFVPVRYQISGSWKE
                     EKWGRVRVSWLLSAICVNGSYEKEKGLQASLRVLWLKLWSMGEEEQTEPPKESKTEAA
                     AKSRDKAETAKETKAEKSKTEQQKTEQPKAESKVQTETEHTAEPKTQTQSKAGPEKTA
                     EKKSSTKPEAKPETKPVQKQEKAIKVAEADQEEDKIEPAEKKSHQWIRKIKAFLEKLK
                     FSFKHFCDKLKDIKTFVREKREWLEDEKNQESLKLLFTQAKKLLAHVWPGWGKGRIRF
                     GLDDPYYTGQILEGASLVYPFIYKSLDLEPVFDEKCLEAEGNFKGRVRVSYLLWLFWG
                     VYRHKHTWKMIRGFLDRE"
     gene            complement(1942..2226)
                     /locus_tag="ACZPCL_13445"
     CDS             complement(1942..2226)
                     /locus_tag="ACZPCL_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163505.1"
                     /translation="MRKGKMLVDTYVITLPESGNHILDILPVSLLTRQERDGKDFLVL
                     GIARGYEEAAQVVRSMVDDMYQKTGAFNWKAYMESLPEKSGAEYGDWQEQ"
     gene            complement(2337..>2665)
                     /locus_tag="ACZPCL_13450"
     CDS             complement(2337..>2665)
                     /locus_tag="ACZPCL_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163506.1"
                     /translation="PECKVLWKTELLLSGSAFASLEYKASEAMADESMISEKLDELFK
                     EIRQSWKELPKVLTRAAMAKCLSRLPITFKTSDEAQAYIRNSLECCTDEIEKEACVRM
                     LRSIME"
BASE COUNT          648 a          689 c          472 g          856 t
ORIGIN      
        1 gcgaacgtta aatctgtcag agtcaaggtt aatggtggaa cccagagaat gtacgtatgt
       61 acttcctgct tacgttcagg ccttgtagaa agagcttaat ttttgaacag agcgtgaaaa
      121 atcccggtag cttcggttac cgggattttt ttgcgttata catttgatta catctgctcc
      181 tgccacaggc aggaatgaat gacaggtcta acagcaaaat aaattatagc ctaaaatcag
      241 gcatccgatc acaaagaaaa aggtaaatat ggtttccggg acaaagaaaa gcactgccat
      301 tccaagcccg aagcaaaaaa gcatcagtcc ccagactctt ttcatagaaa aacagctccc
      361 ttccatttta ttttatgcgg taagggagct gtttgatgct ttttatatca ggctttttta
      421 ccttcgtaag aagctgctgt ttcttttgca atgcctactg tttcttctga tacagccggt
      481 tttcctccta aaaagcggtt gatcagatcc ggagccatat caatcacctt ggtcactgca
      541 tcctggttct ttacattgac cagcttggtc acaccattct ggatcaccag gacagcactt
      601 ggactcatct tggcgctcat tccgccacag cctttgtctt tctgctttga accttctgca
      661 aaagcgcctg cagccatacc acaggtcaca tcgatcagcg gtataataat ggtctctcct
      721 acctggaccg gttctcctac gactgttttc gtagttacaa actgatccat tcctttaaat
      781 aatgattcaa ttgtagatgt aaaatgattt tctgccataa tatacctcta ttctcctgaa
      841 atattctccc gtagatctta aatccccaac ttactcacga tctaaaaaac cccgtatcat
      901 cttccaggta tgtttgtgac gatagactcc ccaaaacagc cataaaagat aggaaacccg
      961 gacacgtcct ttaaaatttc cttctgcttc caggcacttt tcatcaaata ccggctctaa
     1021 gtccaggctt ttatagataa atggatacac cagactggct ccctccagga tctgccctgt
     1081 ataataagga tcatccagtc caaaacggat ccttcctttt ccccagcccg gccatacatg
     1141 tgccagaagc ttttttgcct gggtaaaaag cagttttaag gactcctgat tcttctcatc
     1201 ctccagccat tctctctttt cccggacaaa agttttaata tcttttagct tatcacaaaa
     1261 atgcttaaaa gaaaacttaa gtttttctaa gaaggctttg atttttcgga tccactggtg
     1321 tgattttttc tcagcaggtt ctatcttgtc ttcttcttgg tctgcttctg ctacttttat
     1381 agccttttcc tgcttttgca caggttttgt ttctggtttt gcttctggtt ttgtactgga
     1441 ctttttctca gcggttttct ctggtcctgc ttttgactgt gtctgtgttt ttggctctgc
     1501 cgtatgttca gtctctgttt gcacctttga ctccgccttt ggctgttctg tcttttgctg
     1561 ttctgtcttt gacttttctg cctttgtttc cttcgctgtt tccgctttat ccctgctttt
     1621 ggctgccgct tctgtcttac tttcttttgg aggctctgtc tgttcttctt cccccatgct
     1681 ccaaagtttc agccacagca cccgcagcga tgcctgaagc cctttctctt tttcataaga
     1741 accgtttacg cagatcgccg aaagcagcca ggatactctc acccggcccc atttttcttc
     1801 tttccacgat ccgctgatct gataccttac aggaacaaaa aggacggtac acaataccag
     1861 aagcaggagc ccaaaaagaa ccagcagtat gatccccaga atttttaata taagcaatac
     1921 aatatgcaat cgcctgcccc cttactgctc ctgccagtct ccatattcag cacctgactt
     1981 ttccggaaga ctttccatat aagccttcca gttaaaagca cctgttttct gatacatgtc
     2041 atccaccata gaacggacta cctgcgctgc ctcctcatag ccccttgcga tccccaaaac
     2101 cagaaagtct tttccatctc tctcctgtct ggtaagaaga cttacaggaa gtatatcaag
     2161 tatatgattg ccactttccg gcaaagtaat cacataggta tccacaagca tttttccttt
     2221 tctcattccc tccaggatac tcaccctgtt ccgggaagca ttttcgccca tgtataaatg
     2281 agtccaccag cgcataaaat cccctcttag atcttgttcc attttactta ttttatttat
     2341 tccatgattg agcgcagcat acgcacacag gcctcttttt caatctcatc tgtgcagcat
     2401 tccagactgt tccggatata agcctgggct tcatctgagg ttttaaaggt gataggaagc
     2461 cgtgacaggc attttgccat agccgctctt gtaagaacct ttggaagctc tttccatgac
     2521 tgcctgatct ctttaaacag ctcatctaat ttttcactga tcatgctttc atctgccatc
     2581 gcttcagaag ctttatattc cagtgaagca aaggcactgc ctgataagag aagttctgtt
     2641 ttccagagga ccttacattc cggaa
//
LOCUS       DBMDUA010000287         2634 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_13305_length_2634_cov_2.864193, whole genome shotgun sequence.
ACCESSION   DBMDUA010000287 DBMDUA010000000
VERSION     DBMDUA010000287.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13305_length_2634_cov_2.864193"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            109..810
                     /locus_tag="ACZPCL_13455"
     CDS             109..810
                     /locus_tag="ACZPCL_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIR1163507.1"
                     /translation="MSTLREILGNSQYWGLTLSIAAYLFACWLKQKTKLAFLNPILVS
                     VIVIIAFLLGVGIDYDTYNKGASYLSWLLTPATVCLAIPLYKQLHLLKKHGAAVAVSI
                     TSGVATSAVSIFLMCRVLGLSHTHYVTLLPKSITTAIGMGVSEEAGGIVTLTVISIII
                     TGVLGNMVGETVLKLAKIDHPVAKGLAFGTSAHAVGTAKALELGEVEGAMSSLSIAVA
                     GLLTVIAVPIASKFI"
     gene            975..2573
                     /gene="pepF"
                     /locus_tag="ACZPCL_13460"
     CDS             975..2573
                     /gene="pepF"
                     /locus_tag="ACZPCL_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HIR1163508.1"
                     /translation="MKKRSEAEISTTWKLEDMVPDNETWEKQLKEASGELEKYGEFKG
                     KLAESAENLYQCLKFDDEFSLKAERLYVYARMRSDEDTANDLYQDMFSRAQLLNIRAS
                     ENSAYMVPEILSIPEEILKEYRNSHPGLKHFDRLLDQLLERKSHTLSKEMEQLLAQSY
                     EATQGGSQVFTMFNNADARFPAVHDAEGKEIPLTHGNYIALLENQNRDIRKEAFENLY
                     SVYKQFSNTLSAAFGANVKQAVFYAKARNYTSSRQASLSGNEVPESVYDNLVASVRKS
                     LPLLHRYASLRKKLLGVEELHMYDLYVPMVAEADRHYTFEEAKDIVKTGLAPMGEEYL
                     GLLQEGFDNRWIDVYENEGKRSGAYSWGVYGCHPYVLLNFHGTLNDVFTLAHEMGHSI
                     HTWYSNKNQSYTYSGYKIFVAEVASTCNEALLIRHLLKNSKDKQEKAYLLNYFLESFR
                     GTLFRQTMFAEFEQKAHEMAAAGESMTSQSLCNIYKKLNEDYFGPDTVIDPEIQYEWE
                     RIPHFYTPFYVYQYATGFSAPICS"
BASE COUNT          772 a          547 c          602 g          713 t
ORIGIN      
        1 catttctgat cattatcgca gtttctactg ttgtagtaat gatcgttacc ggaaaagtgg
       61 cacagtttat gatggaatca gcagagaaga aaaaggagaa atgatcaaat gagtacatta
      121 agagagatcc tgggaaattc ccagtactgg ggacttacct taagtatagc agcttatctg
      181 tttgcctgct ggctgaaaca gaagacaaag cttgcctttt taaatccgat cctggtatct
      241 gttatcgtga tcattgcatt tttactggga gtaggaattg attatgatac atataataaa
      301 ggagcatctt atttaagctg gctccttaca ccggcaactg tatgtctggc gatcccgctg
      361 tataagcagc ttcacctgtt aaagaagcat ggtgcagccg ttgcagtaag cattacttcc
      421 ggcgttgcca ccagtgcagt gagtattttc ctcatgtgcc gtgttctggg actgtcccac
      481 acccattatg taacactgct tccaaagtcc attacaactg ctattggaat gggtgtcagt
      541 gaagaagcag gcggtattgt aacactgact gttatcagta ttatcatcac cggtgtttta
      601 ggaaatatgg ttggcgagac tgtacttaaa cttgcaaaga tcgaccaccc ggtagcaaag
      661 ggactggcat ttggtaccag cgcccatgca gtaggtacag caaaagcact ggaattagga
      721 gaagtagaag gtgccatgag cagcctttct attgcagtgg caggtctgct cactgtgatc
      781 gcagtgccga tcgcgtctaa gtttatataa gcgcagagaa atcttcaaat gcgtcactgg
      841 cgcattcttg atatgcatgg caaaacaagc ggctgcgtta gcgcggaaat ctaagtctat
      901 ataaagatat ttcacctgcc ccggcattgc cggggcacat ctgtatttgc aaataaaaga
      961 aaggggttac gtttatgaag aaaagaagtg aagcagaaat ttctaccacc tggaagttag
     1021 aggacatggt cccggataat gagacctggg aaaaacagtt aaaagaagcg tctggtgagc
     1081 ttgaaaaata cggggaattt aagggcaagc tggcagagtc agcagaaaac ttataccagt
     1141 gcctgaaatt tgatgatgag ttcagcttaa aagcagagcg cctgtacgta tatgccagaa
     1201 tgcgttccga cgaagataca gcaaatgacc tgtatcagga catgttttct agggcacagc
     1261 ttttaaatat ccgggcttct gaaaattcag cttatatggt tccggagatc ttatccattc
     1321 cggaagaaat cttaaaagaa tacagaaaca gccatccggg cctgaaacat tttgaccgcc
     1381 tgttagatca gctgttagag cgcaagagcc acacactttc taaagaaatg gaacagttac
     1441 tggctcagtc ctacgaggca acccagggcg gaagccaggt atttaccatg ttcaataatg
     1501 cagatgcccg cttcccggct gttcatgacg cagagggaaa ggaaattcct cttacccatg
     1561 gaaattatat tgctctcctg gaaaatcaga acagagacat ccgaaaagag gcttttgaaa
     1621 acctgtatag tgtatacaag cagttttcca ataccttatc tgcagctttt ggtgccaatg
     1681 taaaacaggc tgtattctat gcaaaggcca gaaattatac ttccagccgt caggcaagcc
     1741 tttccgggaa tgaagttcct gagtctgtat atgacaatct ggtggcatct gtgagaaaaa
     1801 gtctcccact gctccacaga tatgcttctc tgaggaaaaa actcctgggc gtagaagaac
     1861 ttcacatgta tgacttgtat gtgcctatgg tggcagaagc agaccgtcat tatacctttg
     1921 aagaagccaa agatattgta aaaacaggtc ttgcacctat gggcgaagag tatctgggcc
     1981 ttcttcagga aggttttgac aaccgctgga tcgatgtata tgaaaatgaa ggaaaacgaa
     2041 gcggcgctta ttcctggggc gtttatggct gccatcctta tgtattatta aatttccatg
     2101 gaactttaaa tgatgtattt acactggctc acgaaatggg ccattctatc cacacctggt
     2161 actctaacaa gaaccagtct tatacttaca gcggttataa gatctttgtg gcagaggtag
     2221 cttccacctg taacgaggct ctgctgatcc gtcatttatt aaagaacagc aaagataaac
     2281 aggaaaaagc ctatttatta aattatttcc tggaaagctt caggggaacc ttattccgcc
     2341 agaccatgtt tgcggaattt gagcagaaag cccatgagat ggcagcagca ggcgagagca
     2401 tgacttccca gagtctttgc aatatttata agaagttaaa tgaagactat tttggtccgg
     2461 atacagtgat cgaccctgag atccagtatg aatgggagcg gatcccacat ttttataccc
     2521 cattttatgt atatcagtat gctaccggtt tttctgcacc catctgttca taacattctt
     2581 ttactgtcat atttttctcc tcttcatccc catctgcact gttaaacctg cgtt
//
LOCUS       DBMDUA010000288         2634 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_13307_length_2634_cov_1.651402, whole genome shotgun sequence.
ACCESSION   DBMDUA010000288 DBMDUA010000000
VERSION     DBMDUA010000288.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13307_length_2634_cov_1.651402"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="ACZPCL_13465"
     CDS             complement(<1..1626)
                     /locus_tag="ACZPCL_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HIR1163509.1"
                     /translation="MKKKKRTLMHILLLPLLLVVLFQGILPFSMLLSSGVKSIMESNA
                     VDLDSYLVENSKVILENAMIEQWSAVRKESNYLNSVLSDLLKEQRADIEKFTKDKELQ
                     KEYTQRVFPELLESLRRDTTCGVFLVMANDQPIGAPGDFTGFFLRDSDPVTKADTNSD
                     LLLERGDKTLARQWGITLDNSWSPAFRFQGKGIRESDDFFYEPYTAGMAHTGVDMASL
                     GYWSMPFILEDYEMDNHKMITYSVPLVNDGVIYGVVGTEVSVSYLCNTYFRVQDLNRQ
                     QTAGYAIAVDNGDGTYENIAGKGNLYDAVQRENGIFKLESTKHEDLFKVKDSKIGSQE
                     IYALFSKMNLYANNVPYKNTNWVLCGFVAEDSIFSLGDKLYSDILTTIVACAVLGLVI
                     MSVVIRCVTRPVYRLMDSIRSGIAGLKEFAPSGIQEVDELHQVVENLTESEISTEHQL
                     SEEKERYRIAVESSNDLFFTYREKGNTLEIVNSREMDGLWDIKNAKKKILDRYFSRED
                     QKKLLSMLQSHENTIYGQFCCINGNHSQGRWIEI"
     gene            complement(1703..2563)
                     /locus_tag="ACZPCL_13470"
     CDS             complement(1703..2563)
                     /locus_tag="ACZPCL_13470"
                     /inference="COORDINATES: protein motif:HMM:NF024808.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR1163510.1"
                     /translation="MASSPDIPDDGKAFYGRDSMSNYFIIGMKQLGADIFDVKDGQVT
                     IQADKEKVRRLWDNYYVPYVNGYFASMGKFRSDDVKTGDILAYTGSSVSSMYFPDQVV
                     TDEGSHAIDYVVMQPPVLEGGEQINVQQGAGMAVTKSDAQHEYASCEFLKWFTRKENN
                     LRFVCESAYLPVRKDANSMKALDEVIKEKDLKVNAKAYQCLSSILNAYESIHFYTPNC
                     FENGYATRNILDYDLSDKAAQDKEKVDEMVVAGMSRAEAAAEFTGDAAFEEWYDSWYQ
                     KLNDTAHSNQ"
BASE COUNT          726 a          648 c          472 g          788 t
ORIGIN      
        1 gatctcgatc cagcgtccct gtgagtggtt gccattgata cagcaaaact gaccataaat
       61 tgtattctca tgactttgca gcatagaaag gagctttttc tggtcttccc ttgaaaaata
      121 acggtccagg atcttttttt tggcattttt tatatcccac aaaccatcca tttccctgct
      181 gtttacgatc tccagtgtat ttcctttttc acggtatgta aaaaacaggt cattggaact
      241 ttctactgcg atccggtaac gctctttttc ttcactgagc tgatgttctg tgctgatctc
      301 actttctgtc agattttcaa ccacctgatg cagctcatct acttcctgga taccggaagg
      361 cgcaaactct tttaagcctg caattccgct tcggatactg tccataagcc tgtataccgg
      421 tcgtgttacg cagcggatca ccacactcat gatcaccagt cccaaaactg cacaggccac
      481 aatagtcgtc aaaatatcgc tgtacagctt atctcccaga ctgaaaatag agtcctctgc
      541 cacaaaacca cataaaaccc agtttgtatt cttataaggg acattattgg catacaaatt
      601 catttttgaa aacagggcat agatctcctg ggaaccgatc ttagagtcct ttaccttgaa
      661 aagatcctca tgctttgtac tttccagctt aaaaatacca ttttcacgct gtaccgcatc
      721 ataaagatta ccttttcctg caatattttc atacgtacca tctccgttat caacggcaat
      781 ggcgtaaccg gctgtctgtt gcctgttcag atcctgtaca cggaaatagg tattgcacag
      841 ataactgaca gaaacttccg ttcccacaac accatagatc acaccatcat tgaccaaagg
      901 gacagaatat gtgatcattt tatggttatc catttcataa tcttccagta taaaaggcat
      961 ggaccagtaa cctaaactgg ccatatctac ccctgtatga gccatacctg ctgtgtaagg
     1021 ttcatagaaa aaatcatcac tttccctgat accttttccc tgaaaacgaa atgcagggga
     1081 ccaggaatta tccagggtaa ttccccactg acgggccaga gttttatccc cgcgctccaa
     1141 aagaaggtca gagtttgtat ctgccttggt cacaggatca gagtccctga ggaaaaatcc
     1201 cgtaaaatct cccggtgccc ctattggctg atcatttgcc ataacaagaa acacaccgca
     1261 ggtggtatca cggcgcaaag actctaaaag ctccggaaat acacgctggg tatattcctt
     1321 ctgaagttcc ttatcctttg taaacttttc tatatctgcc cgttgttctt ttaacagatc
     1381 agaaagaaca ctattcagat agttgctctc tttgcgcaca gcactccact gctcgatcat
     1441 ggcattttcc agtattacct tgctgttttc caccagataa ctgtcaagat ctactgcatt
     1501 actctccata atggatttaa caccgctgga aaggagcatg gaaaaaggta agatgccctg
     1561 aaataatacc accagtaaaa gaggcagaag taaaatatgc atcaatgttc ttttcttttt
     1621 tttcatgttt tacttccaaa cctcctgcct gtggcatttc tcatcttatt tctgatcctg
     1681 tttccggcct taaatacagg atctactgat tggaatgtgc cgtgtcattt aacttctgat
     1741 accagctgtc ataccattct tcaaacgcag catcgccggt aaattctgca gctgcttctg
     1801 ctcttgacat tccggctact accatttcat ctactttttc tttatcctgg gcagctttgt
     1861 cggaaagatc ataatccagg atatttctgg tggcatatcc gttttcaaag cagttcgggg
     1921 tataaaaatg aatggactca taggcattta aaatactgct taaacactga tacgccttag
     1981 catttacttt caggtctttt tctttgatca cttcatctaa cgctttcatg gaattggcat
     2041 ccttgcgcac cggcagatag gcagactcac atacaaaacg gagattattt tcttttctgg
     2101 taaaccattt taaaaactca caggaggcat attcatgctg ggcatctgac tttgtcactg
     2161 ccattcctgc gccctgctgt acattgatct gttcgcctcc ctctaataca ggaggctgca
     2221 ttaccacata atcaatggca tggctgccct catcggttac tacctgatcc gggaagtaca
     2281 tagaagatac ggaagaaccg gtgtaagcca ggatatcccc tgttttcaca tcatctgaac
     2341 ggaattttcc catacttgca aaatagccat ttacataagg tacataataa ttgtcccaca
     2401 ggcggcggac tttttctttg tctgcctgga tcgtaacctg accgtccttt acgtcaaaga
     2461 tatctgctcc taactgtttc atgccaataa taaagtaatt ggacatggag tctcttccgt
     2521 aaaatgcttt tccatcatca gggatatccg gggaactggc cactcaaaac aaaacgcttt
     2581 ccattataga aaagacactg gaacgacaac aagattactt aatggggaat aaat
//
LOCUS       DBMDUA010000289         2607 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_13477_length_2607_cov_2.598563, whole genome shotgun sequence.
ACCESSION   DBMDUA010000289 DBMDUA010000000
VERSION     DBMDUA010000289.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13477_length_2607_cov_2.598563"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="ACZPCL_13475"
     CDS             <1..320
                     /locus_tag="ACZPCL_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RapZ C-terminal domain-containing protein"
                     /protein_id="HIR1163511.1"
                     /translation="VRFLPNPYYDEHLRPLTGQVQAVRDYVMQGGTADAFLKKLYDMI
                     DFLLPNYINEGKNQLVIAVGCTGGKHRSVTIARALYEHLEAVGEYGIRIDHRDIDKDN
                     KKA"
     gene            353..1303
                     /gene="whiA"
                     /locus_tag="ACZPCL_13480"
     CDS             353..1303
                     /gene="whiA"
                     /locus_tag="ACZPCL_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIR1163512.1"
                     /translation="MSFSSTVKEELSRQLTTARHCQIAEMAAILSLCGRVKISASDHF
                     AIAIHTENLAVARKYFTLLKKTFNINTDVLIRQGLLPARSRTYVVAVREHEDALRVLQ
                     AAKLLDANGEIAEDLSLVHNVIVQNTCCRRAFIRGAFLAAGSISDPEKFYHFEITCAS
                     MGKAKQLQGLMASFGIDARIVLRKRYFVVYVKEGSQIVDLLNIMEAPVALMELENIRI
                     VKEMRNTVNRKVNCETANINKTVSAAVKQMEDIRYICDTVGLESLPDNLKEMAKMRLE
                     RPEATLKELGEALEPPVGKSGVNHRLRKLSLIAQDIRERS"
     gene            1355..1609
                     /locus_tag="ACZPCL_13485"
     CDS             1355..1609
                     /locus_tag="ACZPCL_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1163513.1"
                     /translation="MVKKSIKIELSSGLEARPAAMLVQVASQYDSKIYLEADSKRVNA
                     KSIMGMMTLGLVTGESITVEADGTDEEQAVAEIEKYLQNQ"
     gene            1721..>2607
                     /locus_tag="ACZPCL_13490"
     CDS             1721..>2607
                     /locus_tag="ACZPCL_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR1163514.1"
                     /translation="MAFTHLHVHTEYSLLDGSSKIRELTARAKELGMDSMAITDHGVM
                     YGVIDFYRAAREVGIKPILGCEVYVAPGSRFDRENGAGEDRYYHLILLAENNTGYKNL
                     MKIVSKGFVDGFYYKPRVDLDLLETYHEGIISLSACLAGEVQKYLARGMYEEAKRSAL
                     RYSEIFGKDHFYLELQDHGIPEQKMVNQGLLRLSQETGLELVATNDVHYTFAEDAKAH
                     DILLCIQTGKKVTDEDRMRYEGGQYYCKSEEEMRKLFPYAQEAIDNTHKIAERCNVEI
                     EFGVTKLPKYEVPEGFDSWT"
BASE COUNT          767 a          502 c          690 g          648 t
ORIGIN      
        1 atgtgcgttt ccttccaaat ccatattatg atgaacactt acggcctctt acaggtcagg
       61 tgcaggcagt acgggactat gtgatgcagg gcggaacagc ggatgctttt ttaaagaaac
      121 tgtatgacat gatcgatttc cttctgccaa actacataaa tgaaggaaag aaccagttag
      181 tgattgctgt gggctgtaca gggggaaaac accgttccgt taccattgca agagctttgt
      241 atgagcatct ggaggcagtc ggggaatacg ggatccgtat cgatcacaga gatattgata
      301 aagacaacaa gaaggcatag agcatttgat ttgatgcagc agtgaggtaa aaatgtcatt
      361 ttcttccaca gtaaaagagg aactgtccag gcagcttacc actgccagac attgccagat
      421 cgcggaaatg gcggctatct taagcctttg cggacgggtg aagatatcgg catccgacca
      481 ctttgccatt gccatccata cagaaaatct ggcagttgca agaaaatact ttacattatt
      541 gaaaaaaaca tttaatataa atactgatgt tttgatccgt cagggcctgt taccggcccg
      601 gagcaggact tacgttgtag cagtgagaga acatgaagat gctcttcgtg ttttacaggc
      661 agcgaaatta ctggatgcaa atggtgagat cgcagaagac ctgtctttag tccacaacgt
      721 gatcgtgcag aacacctgtt gcagacgggc gttcatccgg ggagcttttc ttgcagcagg
      781 ttctatcagt gatccggaga agttttatca ctttgagatc acctgtgcgt ctatggggaa
      841 ggcaaagcag cttcagggac ttatggcgtc atttggtatt gatgccagga tcgtattacg
      901 caagcgttat tttgtggtat atgtaaaaga aggcagccag atcgttgacc tcttaaatat
      961 catggaagca ccggtcgctt taatggaact ggagaatata cggatcgtaa aagaaatgcg
     1021 caacacagta aaccgtaaag taaactgtga gaccgcaaac atcaacaaaa cagtttctgc
     1081 ggcggtgaag cagatggaag atatccgcta tatatgtgac acagtggggc ttgaatctct
     1141 tccggataat ttaaaggaaa tggctaaaat gcgcctggaa cggccggaag ccactttaaa
     1201 agaactggga gaagctctgg aaccgcctgt aggaaaatcc ggtgttaatc acagactgag
     1261 aaaattaagt ctcatagcac aggatatccg cgaaaggtct taaacaaggt acctggatgg
     1321 gaagatccca ttggcaggaa tgaggaggat aattatggtc aagaaatcta ttaaaattga
     1381 gttgtcttca ggtctggaag cacgtccggc agctatgctg gtacaggttg caagtcagta
     1441 tgacagcaag atctacctgg aagctgattc taagcgtgta aatgcaaaga gtattatggg
     1501 tatgatgact cttggtctgg taaccggtga gtccattacc gtagaggcag atggtactga
     1561 cgaggagcag gctgttgctg agatcgagaa gtacctgcaa aatcagtaaa gagtgtggga
     1621 gagacaacac ttttatagta cgatataaac aggcaaaaga ggctgctgtg gacgggcggt
     1681 ctctttttaa attttagaag tatttcacaa aggagggttt atggcattta cgcatctgca
     1741 tgtccataca gaatacagtc ttttggacgg ctcttccaag atcagagagc tgactgccag
     1801 ggcaaaagag ctgggaatgg acagcatggc aatcactgat cacggcgtta tgtacggcgt
     1861 catcgacttt taccgggctg ccagggaagt ggggatcaaa cccatccttg gctgtgaggt
     1921 ctatgtggct cccggttccc gttttgacag ggaaaatggt gccggcgagg accggtatta
     1981 ccacctgatc cttttggcgg aaaataatac aggctataaa aacctgatga agatcgtttc
     2041 caagggcttt gtagacggtt tttattataa gccaagagtg gacttagacc ttttagaaac
     2101 ttaccatgaa ggcattatct cattaagcgc ctgccttgca ggtgaggtac agaaatacct
     2161 ggcaagaggc atgtatgaag aggcgaagcg gtctgcttta agatattctg agatctttgg
     2221 gaaagaccat ttttatctgg aacttcagga tcatggtata ccggagcaga aaatggtaaa
     2281 tcagggactg ctgcgccttt ctcaggagac ggggctggaa ctggtggcta ccaacgacgt
     2341 tcattatacc tttgcggaag atgccaaggc ccatgacatt ttattgtgta tccagacagg
     2401 caaaaaggta acagatgagg atcgcatgcg ctatgaagga ggacagtatt actgcaaatc
     2461 cgaagaagag atgcgaaagc tgttccctta tgcacaggaa gccattgaca atactcataa
     2521 gattgcagag cggtgtaatg tggagatcga gtttggtgtt accaagctgc caaaatatga
     2581 ggtaccggaa ggttttgact cctggac
//
LOCUS       DBMDUA010000290         2606 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_13485_length_2606_cov_1.746108, whole genome shotgun sequence.
ACCESSION   DBMDUA010000290 DBMDUA010000000
VERSION     DBMDUA010000290.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13485_length_2606_cov_1.746108"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="ACZPCL_13495"
     CDS             <1..606
                     /locus_tag="ACZPCL_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR1163515.1"
                     /translation="MEGIALLSEKIAETYYDEIPKDQRHGKVQVVASSSFFVPKPFTP
                     FQWARMCTKEEFLERANIVRGKFREMKNFKSLKYNWHEAELTVLEGVLARGDRRVGAV
                     IEEAYRKGAIYDSWSEFFKNEVWMEAFKTCGVDIDFYTIRERSLNELFPWDFIDAGVS
                     KNFLKREWQNALDAKVTPNCRMKCSGCGAAKYGGGVCFEKR"
     gene            666..1409
                     /locus_tag="ACZPCL_13500"
     CDS             666..1409
                     /locus_tag="ACZPCL_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HIR1163516.1"
                     /translation="MKIRIKFRKYGTMKFIGHLDVMRYFQKAIRRSEVNIRYSEGFSP
                     HQIMSFAAPLGVGITSKGEYVDIEVLDTENSKKMIDRLNAVMSEGFEILEYKLLPDDA
                     ANAMSIVAAADYSLVFRPGYEPEKESPEEWFEKLVRFFDADHVMIMKKTKKGEREMDL
                     KPFVYELNVRKNGEVPALFMKVSAGSAANIKPELVLDAYYASLGEERPQFAFMTEREE
                     VYADTATAEEAEAGTHKFVTLGDLGQDIE"
     gene            1409..2599
                     /locus_tag="ACZPCL_13505"
     CDS             1409..2599
                     /locus_tag="ACZPCL_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HIR1163517.1"
                     /translation="MDKLVITRLQGKICTASVSDRKVTGITLEEEDKASILNNIYIGK
                     VQKVVTGINAAFVDLGVTIGYYSLDENKEHLFTQQGRNGKLRPGDEILVQVSRDAVKT
                     KAPVLTANLSLPGRFCVLTAGKTGIGFSAKLTDTTWKNQIRKMLPEKEFEDFGIIIRT
                     NARSGDPEQILEECETLKIRLKQLLKESSSRTCYSCLYKNLPAYITGIRDSYAGKLEE
                     IITDCPDLYDQLKEYLENSQKEDAEKLKLYTDDLLSLGKLYSLENALSKALDKRVWLK
                     SGGYLVIEPTEAMVVIDVNTGKYEGKKKLEETVLKINLEAAAEIARQLRLRNLSGIIM
                     VDFIDMNQEANKEALMDALRAAVAKDPVKTVVVEMTRLNLVEITRKKLRRPLYEQVAN
                     STRE"
BASE COUNT          823 a          491 c          643 g          649 t
ORIGIN      
        1 atggaaggga tcgcccttct ttctgagaag atcgcagaga cttactatga tgagattccg
       61 aaagaccaga gacatggtaa agtccaggta gttgccagct cctctttctt tgttccaaag
      121 ccatttaccc cattccagtg ggcaagaatg tgtaccaagg aagagttctt agagcgtgcc
      181 aatatcgtcc gcggcaagtt ccgtgaaatg aagaacttta agagtttaaa atataactgg
      241 catgaagcag aactgactgt tttagagggt gttcttgcaa gaggcgaccg ccgtgtaggc
      301 gctgtgatcg aagaagcata ccgcaaaggc gctatctatg attcctggtc cgagttcttt
      361 aaaaatgaag tctggatgga agcatttaag acctgcggcg tagacatcga tttttacacc
      421 atcagagagc gcagcttaaa cgagctgttc ccatgggatt tcattgatgc cggtgtttcc
      481 aagaatttct taaagagaga atggcagaat gcactggatg caaaggtgac tccaaactgc
      541 cgtatgaaat gttctggctg cggtgctgcc aaatacggcg gcggtgtctg ctttgaaaaa
      601 cgataagacc gcaaatagtg caaattaaca gattaatgta aataaaagga acaaaacaaa
      661 caatgatgaa aattcgtata aaatttcgta agtacggcac catgaaattc atcggccatc
      721 tggacgttat gcgttacttc cagaaggcga tccgtcgatc agaggttaat atccgttaca
      781 gcgaaggctt cagtcctcac cagatcatgt cctttgctgc acctttaggt gtaggtatca
      841 ccagcaaagg cgaatatgtg gatattgagg tgttagatac tgaaaattca aaaaagatga
      901 tcgaccgttt aaatgcagtg atgtcagaag gctttgagat cctggagtac aagcttcttc
      961 ctgacgatgc cgccaatgcc atgtctatcg ttgcagctgc cgactacagt cttgttttcc
     1021 gccctggtta tgaaccggaa aaggaaagcc cggaagaatg gtttgagaaa ctggtccgtt
     1081 tttttgacgc tgatcatgtg atgatcatga aaaagacaaa aaaaggcgag agagaaatgg
     1141 atttaaaacc ttttgtctat gaactgaacg tgcgtaaaaa tggggaggtt cctgcgctct
     1201 ttatgaaggt gtctgcaggc agtgctgcaa acattaagcc ggagcttgtt ttagatgcat
     1261 actatgcttc tttaggagaa gaacgtcctc agtttgcttt tatgacagag agagaagaag
     1321 tttatgcaga tacagccaca gcagaagaag ctgaggctgg cacacataaa tttgtgacac
     1381 ttggagatct gggacaggat atagaataat ggataaatta gtcattaccc gtttgcaggg
     1441 aaaaatatgt acagcttctg tttctgacag aaaggtgaca ggtatcaccc tggaagagga
     1501 agataaggca tctatcctta ataatattta cattggaaag gtccagaagg tagttacagg
     1561 cattaacgca gcctttgttg atctgggcgt gaccattggc tattatagtc tggatgaaaa
     1621 taaagaacat ttatttaccc agcagggaag aaacggtaaa ttaaggcccg gagatgagat
     1681 tttagtccag gtaagccggg atgcggtaaa aacaaaagca ccggtgctta ctgctaatct
     1741 gtccctgcct gggcgctttt gcgttcttac agctggaaaa acaggcattg gtttttctgc
     1801 aaaactcacc gatacaacct ggaaaaacca gatccggaaa atgctgccgg aaaaagagtt
     1861 tgaagatttt ggcattatta taaggaccaa tgccagatcc ggtgatccgg aacagatctt
     1921 agaagagtgc gaaacattaa aaatcagact gaagcagcta ttgaaggaaa gttccagccg
     1981 tacctgctac agctgtctgt acaaaaatct tcctgcctat attacaggca tccgcgactc
     2041 ttatgcaggc aaactggagg agatcatcac agattgtcct gatctctatg accagctaaa
     2101 agagtatctg gaaaacagcc agaaggaaga tgcggaaaaa ttaaaactgt atacagatga
     2161 tctgctttct cttggaaagc tttacagtct ggaaaatgct ctttcaaagg cacttgataa
     2221 acgggtatgg ctgaagtccg gtggctatct ggtgatcgag ccaacagaag ccatggtagt
     2281 gatcgatgta aatacgggca aatacgaagg caaaaagaag ctggaagaaa ccgtattaaa
     2341 gattaattta gaggcagcag cagaaattgc aagacagttg cgtctgcgca acctttctgg
     2401 tattattatg gtggatttta ttgatatgaa ccaggaagca aacaaagaag cgttaatgga
     2461 cgctttacgg gcagctgtcg caaaagaccc ggtaaaaaca gttgtagtag aaatgacccg
     2521 tctgaattta gtggagatca cccgtaaaaa gttgagaaga ccactgtatg aacaggtggc
     2581 taacagtaca agggaatagg ggaaaa
//
LOCUS       DBMDUA010000291         2598 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_13539_length_2598_cov_2.804165, whole genome shotgun sequence.
ACCESSION   DBMDUA010000291 DBMDUA010000000
VERSION     DBMDUA010000291.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13539_length_2598_cov_2.804165"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="ACZPCL_13510"
     CDS             complement(<1..1184)
                     /locus_tag="ACZPCL_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HIR1163518.1"
                     /translation="MEITRDIIYAGVNDHSVDLFEGQYKVPNGMCYNSYVICDEKCAV
                     MDTVDARFTHEWLDNLSGILKGKAPDYLIIQHMEPDHSANIYNFMKNYPNTTIVSNAK
                     AFAMMGQFFPDMNLEGHTLEVKNGDTLTLGSHVLTFVFAPMVHWPEVMVTYDSTDKVL
                     FSADGFGKFGALDAEEDWDDEARRYYIGIVGKYGAQVQKLLKAAAALDIQTICPLHGP
                     VLKENLGHYLEKYNIWSSYEAESEGVVIAYTSVYGNTKKAVELLASRLREKNCPEVVV
                     CDLARMDLSKAVENAFRYGKLVLATTTYNAEIFPFMRTFIDHLTERGYCKRTIGLIEN
                     GSWAPLAAKTMKSMFEKSKDITWLKDKITIQSSLSSENREQIDRMAAELCEEYIARSQ
                     VTA"
     gene            complement(1275..1751)
                     /locus_tag="ACZPCL_13515"
     CDS             complement(1275..1751)
                     /locus_tag="ACZPCL_13515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1163519.1"
                     /translation="MRVLFAVRKFKIGEYSMIRSMKQSDLDPVALIWLDTNIKAHDFI
                     PASYWRDHFEAVRGMLAEAEIYVWEEEGSGKVQGFVGLDGEYIAGIFVQSQSWSQGIG
                     GRLLDYIKKQHSRLTLRVYAKNTRAVRFYQRESFRIQMEETDEMTGQKEYVMLWED"
     gene            complement(1902..2331)
                     /locus_tag="ACZPCL_13520"
                     /pseudo
     CDS             complement(1902..2331)
                     /locus_tag="ACZPCL_13520"
                     /inference="COORDINATES: protein motif:HMM:NF045169.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          695 a          654 c          436 g          813 t
ORIGIN      
        1 gccgtgacct gagaacgggc aatatactcc tcacagagtt cagcagccat tctgtcaatc
       61 tgctctctgt tctcactgct cagtgaggac tgaatggtaa ttttatcctt taaccaggta
      121 atatcctttg acttttcaaa catagacttc atggtctttg ccgccaaagg tgcccaggaa
      181 ccattttcaa tcaggccgat ggtgcgcttg cagtatcccc gctctgtcag atggtcaata
      241 aaggttctca taaacgggaa aatctccgca ttgtaagtag tagttgccag taccagcttt
      301 ccgtagcgga aggcattttc cactgccttt gacagatcca tacgggccaa gtcgcagact
      361 actacctccg gacaattttt ttcacgcagt ctggaggcca aaagctcaac tgccttcttt
      421 gtatttccat agacagaggt atacgcaata accacaccct ctgactctgc ctcataggag
      481 gaccagatat tatatttttc aagataatgc cccagatttt ccttgagaac cggtccgtga
      541 agagggcaga tcgtctggat atcaagggct gcagctgcct ttaaaagctt ctgcacctgt
      601 gcaccgtatt ttcccacaat accgatataa tagcggcggg cttcatcgtc ccagtcctcc
      661 tctgcatcca gcgcaccgaa ttttccaaaa ccatccgcag aaaacagcac cttatctgta
      721 ctgtcatatg tcaccataac ctccggccag tgtaccatag gcgcaaatac aaaggtcagt
      781 acatgagaac ccagagtaag cgtatctccg tttttcacct caagagtatg tccctccaaa
      841 ttcatatccg gaaaaaactg tcccatcatg gcaaacgcct tggcgttgga tacaatggtt
      901 gtattgggat aatttttcat gaaattataa atatttgcgg aatgatccgg ctccatatgc
      961 tggataatca gataatccgg cgcctttccc tttaaaatac cggacagatt gtccagccat
     1021 tcgtgagtga atctggcatc aaccgtatcc ataacagcac acttctcatc gcaaatcaca
     1081 taggagttat agcacatccc gttgggaacc ttatactgtc cttcaaaaag atctacgctg
     1141 tgatcattaa cgcctgcata aataatatct ctggtaattt ccattattat gcccctttct
     1201 tcgtgatagc tttattttac cacgaatatg acgatttttc tattaaaatc aggtctcata
     1261 ttgttaattt tttattaatc ctcccagagc ataacatatt ccttctgccc tgtcatctca
     1321 tcggtttctt ccatctgaat ccggaagctc tccctctgat aaaaacgtac agctctggta
     1381 ttttttgcat ataccctcag agtcagtctg gaatgctgct tttttatata gtcaaggaga
     1441 cggcccccta tcccctgtga ccagctctgg ctttgcacaa aaatccccgc aatatactct
     1501 ccgtccagtc ccacaaagcc ctgaactttt cctgaaccct cttcttccca tacataaatt
     1561 tcagcctctg caagcattcc cctcaccgct tcaaaatgat ccctccagta tgaggcagga
     1621 ataaaatcat gtgccttaat atttgtatcc agccaaatca acgcaaccgg atcaagatct
     1681 gattgtttca tactacgtat catactatac tctccaattt tgaattttct aacagcaaaa
     1741 agcacccgca tcgtttaaac catgcaggcg cttttaattt acaacttttt attggttggt
     1801 gcaagttttt tatttctttt tcaagattta ttatactgct tatgcttata aatatatcag
     1861 aacatttttc caagtgtaca gatatggcta aaaattacat cttaaatata acggcaaata
     1921 tgatccactg ctatatctga aaacccatag gcttccgcct ttgtcatttt tccattgcaa
     1981 acctctatga cttcctttaa caatcgttct cccgcttctt caatcgtaca ggttccttcc
     2041 attatctcac atacacctat atcaatgtta tcatgcatac gttcaaacgt atctcgattt
     2101 ccggttactt taagaactgg tgccagcgca tgtcccgttg gtgttcccct gcctgtggta
     2161 aacacaataa gactgccccc ccgctaccat tgaggtaaca gatgcaatat catagccagg
     2221 agtatccata ataattgcgc cttgttttgt aggtcttgct gtctcaggca gaacttccac
     2281 aatgggcctt gtacctcctt tacggataca tcccaaactt ttttcttcca atgtagataa
     2341 tccccctgtt ttatttcccg gtgtcggctg gcctgtccgg cagtcctatc cggctaaagc
     2401 cagatgcttc tcataatctt tacaaatctg gataatctga ttatgaatct caggagttgc
     2461 accacgtttt gctcacttag acaaatttta ttatctattt acaatagtta tatgatttag
     2521 aagcagaagt caacattttt cattattttc tttataaagt tgtatgtttt atagctattt
     2581 ttacattagt tgtaattt
//
LOCUS       DBMDUA010000292         2589 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_13602_length_2589_cov_2.077572, whole genome shotgun sequence.
ACCESSION   DBMDUA010000292 DBMDUA010000000
VERSION     DBMDUA010000292.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13602_length_2589_cov_2.077572"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="ACZPCL_13525"
     CDS             <1..929
                     /locus_tag="ACZPCL_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR1163520.1"
                     /translation="LLIPTGLHHLVYTPFLYTKLGGTEQIAGQVYEGCRNIYYAEMAD
                     PSIPILSSSVVWDARGLSKMFGLVGACLAMYHTARPENRQKIKAILIPAAFTSFIAGV
                     TEPIEFSFLFVAPILFVAHAVLSGLGMVVFNILNCRAIGPNGFIDFLLYNIPLGIGKT
                     HWPIYLLIGVAEFVIYYFLFRFLITKLNLKTLGREDNGMEMKLHTKAEYKEKTAKAST
                     EKTAASEKGEINAKLIVEALGGPDNIEKVDNCFTRLRLIVKDSSIVKDEVLKQKTGAA
                     GVFKKNETVQVVYGLQINQVRKAVDKELGLDQ"
     gene            949..2283
                     /locus_tag="ACZPCL_13530"
     CDS             949..2283
                     /locus_tag="ACZPCL_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HIR1163521.1"
                     /translation="MDGKKFNVVIAGGGSTYTPGIVKMMLEHQEDFPLNSVTLYDNDA
                     ERQAVIGEALEILMKEEAPQIHFAYTTDPETAFTGKDFCMAHIRVGKYAMRRLDERIP
                     MKYGVVGQETCGPGGIAYGMRSITGMLEIIDYMEKYSPDCWMLNYSNPASIVAEACRV
                     LRPKAKVINICDMPVGTRRRMAYIVGKEYDDLDVRYFGLNHFGWWTSVKDKTTGEDYM
                     PKLIKYVAENGYLTQKAIETQHMDASWQATHRKAKDLLALDPQFLPNTYLKYYMYPDY
                     VVEHTDPNHTRADEVIEGREKEVFGAAREIIRKGTATGGEFTVDNHAAFIVSLARALA
                     YNTHEIMLCIVENKGAISNFDDDAMVEVPCVVGFNGPEPMCQGEIPTFERGMMQEQLA
                     VEKLVVQAYVEHSYLKLWQALTLSKTVPSASVAKQILDDLIEANKDYWPELK"
     gene            2297..>2589
                     /locus_tag="ACZPCL_13535"
     CDS             2297..>2589
                     /locus_tag="ACZPCL_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1163522.1"
                     /translation="MGLFGWLKKAPEIKKAEFVSPATGKIIPVDQVEDETFASKILGD
                     GFAVELTDGEVIAPFDGEVTAAFPTGHAYGLKREDGLECLIREQELSTEKNWYS"
BASE COUNT          818 a          454 c          632 g          685 t
ORIGIN      
        1 ggcttctgat ccctacagga cttcatcatc tggtttacac cccgtttctt tacacaaaac
       61 tgggtggtac agagcagatt gcaggtcagg tatacgaagg ctgccgaaat atttactatg
      121 cggaaatggc agatccgtct atcccgatct tatcctcttc tgtagtatgg gatgcaagag
      181 gactttcaaa aatgtttggt ctggttggcg catgccttgc aatgtatcac acagcaagac
      241 ccgaaaaccg tcagaagatc aaagcaatcc tgatcccggc tgcatttaca tcctttattg
      301 ccggtgtaac agaaccaatt gaattctctt tcctgtttgt agcgccgatc ttatttgtag
      361 cccatgcagt attaagcgga cttggaatgg tagtatttaa tatattaaac tgccgtgcca
      421 tcggaccaaa tggatttatt gatttcctcc tgtataatat cccgttagga attggaaaga
      481 cacattggcc aatttatctt ttaataggag ttgcggaatt tgttatttat tatttcttat
      541 tccgtttcct gatcaccaag ctgaacttaa aaacacttgg gcgtgaagat aatggaatgg
      601 aaatgaaact tcatacaaag gctgagtata aagaaaagac tgcaaaagca tctacagaaa
      661 aaacggcagc atctgaaaaa ggggagatca atgcaaagct gatcgtagag gcattaggtg
      721 gtccggataa cattgaaaaa gtagataact gttttacacg tttacgtctg atcgtaaaag
      781 acagttcaat tgttaaggat gaggttttaa agcagaaaac aggagcagca ggcgtattta
      841 agaaaaatga gacagttcag gtagtttacg gtcttcagat caatcaggta agaaaggcag
      901 tagacaagga actgggcttg gatcagtaat agaagggaga tcataaaaat ggacggaaaa
      961 aagtttaatg tagtgattgc cggaggcggc agtacttaca caccaggaat tgtaaaaatg
     1021 atgctggaac atcaggaaga ctttccgctg aattcagtaa cactttatga taatgatgca
     1081 gagcgtcagg ctgttatagg tgaagcactt gaaatattaa tgaaagaaga ggctccacag
     1141 attcatttcg catatacgac cgacccagaa acagcgttta caggaaagga tttctgtatg
     1201 gcacatatcc gtgtaggaaa atatgcaatg cgcaggttgg atgagcggat cccaatgaaa
     1261 tatggcgttg ttggacagga aacctgtggc ccgggcggta ttgcttatgg aatgagaagc
     1321 attaccggta tgctggagat tattgattat atggaaaaat attctccgga ttgctggatg
     1381 ctcaattact ctaatcctgc atccatcgtt gcggaggcct gccgtgtatt aagaccaaag
     1441 gcaaaggtga tcaatatctg tgatatgccg gtgggtacca gaagaagaat ggcatacatt
     1501 gtaggaaaag agtacgatga tctggatgtt cgctattttg gtttaaatca ctttggctgg
     1561 tggaccagtg taaaggataa aacaacgggc gaagattata tgcctaaatt aataaaatat
     1621 gttgctgaaa acggttatct tacccagaaa gcaatcgaaa cacagcatat ggatgcaagc
     1681 tggcaggcga cacacagaaa agcgaaagat cttctggccc tggatccaca gtttttacca
     1741 aatacatact taaagtacta tatgtatccg gattatgtag tagaacatac agatccaaac
     1801 cacacccgtg cagatgaggt tattgaaggc agggagaaag aagtatttgg cgcagcaaga
     1861 gagatcatcc gcaaaggaac tgctacaggc ggtgagttta ctgttgataa tcatgcggct
     1921 tttatcgtat cactggcaag agcgctggct tataatacac atgagatcat gctgtgtatt
     1981 gtggaaaata agggtgccat cagcaatttt gatgatgacg ctatggtaga ggttccttgt
     2041 gttgtaggtt ttaatggtcc ggagccaatg tgccagggag aaatcccaac ctttgaaaga
     2101 ggtatgatgc aggaacagct tgcagttgaa aaactggttg tacaggcata tgtagaacac
     2161 agctatttaa aactgtggca ggcgcttact ttatcaaaga cagttccaag tgcttctgta
     2221 gcaaaacaga tcctggatga tctgattgaa gcaaacaagg attactggcc tgaattaaag
     2281 tgatcaggag gtgcgtatgg gattatttgg ctggctgaaa aaagcaccag agattaaaaa
     2341 agcagaattt gtatctccgg ctacaggaaa aattattccg gtcgatcagg tagaagatga
     2401 gacatttgct tctaaaatcc taggagatgg ttttgctgtg gaacttacag atggtgaagt
     2461 aattgctcca tttgacggcg aagtgacggc tgcatttcct acaggacatg cctatggttt
     2521 aaaacgtgaa gacggtttag aatgtttgat ccgggaacag gaactttcga cggagaaaaa
     2581 ctggtattc
//
LOCUS       DBMDUA010000293         2570 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_13720_length_2570_cov_2.507898, whole genome shotgun sequence.
ACCESSION   DBMDUA010000293 DBMDUA010000000
VERSION     DBMDUA010000293.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13720_length_2570_cov_2.507898"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            143..2005
                     /locus_tag="ACZPCL_13540"
     CDS             143..2005
                     /locus_tag="ACZPCL_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR1163523.1"
                     /translation="MNELENNRSRNSSTGRSRARGTNRSGASGRSASSSRSSSSRSSY
                     GTSSGRRSSSSGYSSRSGGSHSTRRSSGYHRKKGPNYKIIAIAGVVLIVFITGISLAL
                     KGKGSNTDTAGTETVIESEAETEMQKEVQVDGISITGMSKSEAKAAILKEFPWSMTVE
                     YESDQYQVTDLMAEKVEALLDEIYKDGSDPQESYTLDTSGLDEQVKAEAAACAAKWDK
                     KAKNGSIDKFDTASGKFVFAGEENGFAIDQDKLASGISQALNDKKFDATITATGSTVA
                     PEISASSAKEKYKTISTFTTTTTANQNRNTNVRLAAEAINGTVIKPGQEFSFNGTVGQ
                     RTEAKGYKGAAAYNNGEVVQEIGGGVCQVSTTLYNAVFKAGLKISSRRSHTFEPSYVT
                     PGRDATVSWDQPDFKFINNSSTAIGLRASYADQKVTVAVYGIPILEDGVTWDLESKKV
                     EDLGTPNPTYEEDQTLQPGAEVTKSKGSAGSRWETYKIVYKNGKEVSRELDHKTTYKG
                     HTPVVRRNSSGVVLNPSETTTQATTAPSTVDGMPDGYVPSETTAASTTVAPNATTAAN
                     HTTAAPSHSAEATTAATTAASPAPVQTTAAATTTAAENGGPGDSGDVVIPVKPD"
BASE COUNT          881 a          504 c          609 g          576 t
ORIGIN      
        1 ttaacaggaa tgttacaaac agaaaaaaag ggtaagaaat cacctgaaaa aaagtgtaaa
       61 aatcggttcc attttaaata aagtatgcta tactacaagc tgtgattaaa aataagctaa
      121 aaaatgtaac aggtgaaaag aaatgaatga gttagaaaac aacagaagtc gaaattcctc
      181 tacaggcaga agcagggcca gaggtacaaa tagaagcggt gcatcaggaa gaagcgcttc
      241 atcttcaagg agcagttctt ccagaagcag ttatggaaca tcttccggcc gcagaagcag
      301 ttcttctggt tattcttccc gttcgggagg aagccattcc accagacgga gcagtggata
      361 tcacagaaag aaaggcccga attacaagat cattgccatt gcaggtgtag tcctgatcgt
      421 atttatcaca ggaatttccc ttgctcttaa gggaaaaggt tccaatacag atactgcagg
      481 aacagaaaca gtgatcgaaa gcgaagctga gactgaaatg caaaaagagg tccaggtgga
      541 tggtatctct attacaggaa tgtccaagag cgaagcaaaa gcagctattt taaaggaatt
      601 cccgtggagt atgacagtag aatatgagtc tgaccagtat caggtaacgg atctgatggc
      661 agaaaaggtg gaagcactgt tagatgagat ctacaaagat ggcagtgatc ctcaggaaag
      721 ctatacactg gacacatctg gtctggatga gcaggtcaag gcagaggcag cagcctgcgc
      781 agcaaaatgg gacaagaagg caaagaacgg ttccattgac aagtttgata cagcaagcgg
      841 taaattcgta tttgcaggtg aggaaaatgg ttttgccatt gaccaggaca agctggcatc
      901 aggtatttct caggctttaa atgataaaaa gtttgatgca acgatcactg caaccggaag
      961 cactgtagca ccggagatct ctgcatccag cgcaaaagag aaatataaga ccatcagcac
     1021 ctttacaacg accacaactg cgaaccagaa ccgtaatacc aacgtgcgtc tggcagcaga
     1081 ggctattaat ggtacagtta tcaagccggg acaggaattt tcttttaatg gtactgtagg
     1141 acagcgtacc gaagctaagg gatacaaagg cgcagctgct tataataacg gagaagtagt
     1201 acaggagatc ggtggtggtg tatgccaggt ttctactact ttatataatg cagtatttaa
     1261 ggctggttta aagatcagct cccgccgttc ccacacattt gagccaagct atgtaacacc
     1321 gggccgtgac gcaactgtaa gctgggatca gccggatttt aagtttatta ataattcaag
     1381 tacagccatt ggtttaagag caagttatgc agaccagaaa gtaacggtag cagtttatgg
     1441 tatcccgatc ctggaagatg gagttacctg ggatttagag tctaaaaagg tagaagatct
     1501 gggtacacca aatccaacct atgaggaaga ccagactctt cagccaggcg cagaagtgac
     1561 caagtccaag ggaagtgccg gaagcagatg ggaaacctat aagatcgttt ataagaatgg
     1621 aaaagaagtg tccagagaac tggatcacaa gaccacttat aagggacata cccctgtagt
     1681 ccgccgtaat tccagtggtg tagtattaaa tccatctgag acaactacac aggcaaccac
     1741 cgctccatct accgtagatg gaatgccaga tggctatgtt ccgtctgaga caaccgcagc
     1801 cagcacaacg gtagcaccaa atgcgacaac tgcagcaaat catacaactg cagcgccttc
     1861 tcattccgca gaagcaacca cagctgcaac cacagccgct tccccggcac cggtacagac
     1921 aactgcagca gctaccacca cagcagcaga aaatggcggc cctggagata gcggagatgt
     1981 ggtaatacct gtaaaaccag actagaagcc agatcggaaa atagaataaa acagatcttg
     2041 tggatcacaa aatcccctgt caccggcaga tgatgctggg tggcagggga tttttgcgga
     2101 tatgggatgg aaatgttttc tgcttttttg cgacaaaaga attaaaatat tttttcatga
     2161 aatttgaaat aatacaaaaa agttgttgac aaacggtttg ggatttgcta ttataatctt
     2221 cgcgcttgag agagcagcaa tcgtttcaaa tgaaacgaaa aaataaatta aaaaagttgt
     2281 tgacaaatga aaaacaaccg gttataataa atgaccgttg cagcaaacga gtctgacaac
     2341 gtaatgagat ccttgataat tgaagattaa acagtatgta aaaccctgaa aattcagaaa
     2401 ataaaaggtc tggtttagac ttttggattt ttgagaaaat tcagaacaaa aaccaagtaa
     2461 aacgggttag ataaattagc caagctaagt aatccggact tcgatcaact ttttaacatg
     2521 agagtttgat cctggctcag gatgaacgct ggcggcgtgc ttaacacatg
//
LOCUS       DBMDUA010000294         2566 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_13746_length_2566_cov_2.125761, whole genome shotgun sequence.
ACCESSION   DBMDUA010000294 DBMDUA010000000
VERSION     DBMDUA010000294.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13746_length_2566_cov_2.125761"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="ACZPCL_13545"
     CDS             complement(<1..183)
                     /locus_tag="ACZPCL_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large ribosomal subunit protein bL28"
                     /protein_id="HIR1163524.1"
                     /translation="MAKCAICEKAAHFGNKVSHSHRRSNKMWKANVKSVKVKVNGGTQ
                     RMYSLINRANIAVSEIN"
     gene            350..706
                     /locus_tag="ACZPCL_13550"
     CDS             350..706
                     /locus_tag="ACZPCL_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR1163525.1"
                     /translation="MKGRISNKMGEIQINPDVIALYAGTIAVECFGIVGMAAVSMKDG
                     LVKLLKRESLTHGINVDIKENKISIDFHVIVSYGVSISAVSDNLIESVKYRVEEFTGM
                     EVEKINIFVEGVRVID"
     gene            720..2447
                     /locus_tag="ACZPCL_13555"
     CDS             720..2447
                     /locus_tag="ACZPCL_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR1163526.1"
                     /translation="MGISTINAGMVKNAFLAGAKGLAAKKEWINELNVFPVPDGDTGT
                     NMTLTIMAAAKEVAGLENPSMDQLAKAISSGSLRGARGNSGVILSQLLRGFTKEIKTV
                     DEIDVTTLANAMMRGTETAYKAVMKPKEGTILTVAKGMADKALEMAVETDDIEEFAKA
                     VIEEGDRVLNLTPEMLPVLKQAGVVDSGGQGLMQVIKGAFDGLTGKVTDFTLEEGTAS
                     AHASEAKPAVQTGNGASRTDIDTADIKFGYCTEFIIKLEKEYTDEDEAELKKYLGSIG
                     DSLVVVSDDEIVKIHVHTNHPGLAFEKGLTYGSLSRMKVDNMREEHEERVIQDSERLA
                     KEQAQADAAKTEETQPEEQTERKEYGFIAVSCGDGLSEIFKGIGTDYLIEGGQTMNPS
                     TEDMLNAIAHVNADHIFILPNNKNIIMAANQARDLTEDKEIIVIPSKTVPQGITALVN
                     FMPDLTSQENLENMTAEMERVKTAQITYAVRTTNIDGMDIEKGDIMAIGDKGMLAVEH
                     SPEEAAKAALKAMLDDESELVTIYYGCDVKEEDAEKLKEEAESLFPDKELELQYGGQP
                     IYYYMISAE"
BASE COUNT          835 a          461 c          624 g          646 t
ORIGIN      
        1 gttgatttca gaaaccgcta tatttgctcg gttaatcaaa gagtacattc tctgggttcc
       61 gccatttact ttaaccttaa cagattttac gtttgctttc cacatcttgt tggaccgtct
      121 atgggaatgg ctcactttgt ttccgaaatg agctgccttt tcacagattg cacacttagc
      181 catactattg cacctcctta aacgttactt ggattttata gtagaaaaat ccacaacaat
      241 ggtataatac cagaaactat caaaatttgc aagcgaaaaa ataaaaattt atttcttttt
      301 ttagtgaaat agtgtatact gtcactagaa aaagatagga ggctcgtata tgaagggaag
      361 aatcagcaat aaaatgggcg agatccagat caacccggat gtgatcgctt tatatgcagg
      421 aaccatcgct gtggagtgtt tcggtattgt cggaatggca gctgtaagca tgaaagacgg
      481 acttgtaaaa cttttaaaaa gggaaagtct gacccatggt attaatgtgg acattaagga
      541 aaataaaata tcaattgatt ttcatgtgat cgtatcctat ggggtcagca tatcagcagt
      601 atctgacaac ttaattgaaa gcgtgaaata cagagtagaa gaattcaccg gcatggaggt
      661 agaaaagatc aatatcttcg tggaaggtgt cagagtgatt gactaggagg aagtaacctg
      721 tgggtataag tacaattaat gccggaatgg taaaaaatgc gtttctggca ggagcaaaag
      781 gccttgcggc caagaaagaa tggattaatg agctgaatgt atttccagtt ccggatggag
      841 atacaggaac gaacatgacc cttacgatca tggctgcagc aaaagaagta gcaggacttg
      901 aaaatccttc tatggatcag ctggcaaagg ccatctcttc cggttcctta agaggagcaa
      961 gaggaaattc cggcgttatc ctttcccagc ttttaagagg atttacaaaa gaaatcaaaa
     1021 ccgttgatga gatcgacgtt accacactgg caaatgccat gatgcgcgga accgagactg
     1081 cttataaggc agttatgaag ccaaaagaag gaaccatctt aaccgttgcc aagggaatgg
     1141 cagacaaagc tctggaaatg gcagtagaaa cagatgacat tgaagagttt gccaaggcag
     1201 tgatcgagga aggtgacaga gttttaaatc tgactccgga aatgcttcct gtattaaaac
     1261 aggcaggtgt agtagactcc ggcggtcagg gactgatgca ggtgatcaag ggtgcttttg
     1321 atggtctgac cggcaaagtt accgatttta cactggaaga aggaacagct tctgctcatg
     1381 catcagaggc aaagccggct gtacagacag gaaacggcgc ttccagaacc gatattgata
     1441 cagctgatat taaatttggt tactgcaccg agtttattat taaattagaa aaagaataca
     1501 ccgatgagga cgaagcggaa ctgaagaaat acttaggttc tattggtgac tctttagtag
     1561 tcgtttctga tgatgagatc gtaaagatcc atgtacacac caatcatccg ggacttgctt
     1621 ttgagaaagg tcttacctac ggttctttat cccgtatgaa agttgacaat atgcgtgaag
     1681 agcatgaaga acgtgtgatc caggactctg aacgtcttgc aaaagagcag gcacaggctg
     1741 atgcggcaaa aacagaagag acacagccgg aagagcagac agagcgcaaa gaatacggtt
     1801 ttatcgcagt atcctgcggt gatggattaa gtgagatctt taagggaatt ggcactgatt
     1861 atctgatcga aggcggccag accatgaatc caagtacaga ggatatgtta aatgccattg
     1921 ctcatgtaaa tgcagatcac atttttatcc ttcctaataa taagaacatt atcatggctg
     1981 caaaccaggc aagagatctg actgaggata aggagatcat cgttatcccg tcaaagactg
     2041 ttcctcaggg aattacagca ttagtaaact ttatgcctga tcttacttct caggaaaacc
     2101 tggaaaatat gacagctgag atggaacgtg taaagactgc ccagatcact tatgcagtgc
     2161 gtactaccaa tatcgacggc atggatattg aaaagggcga tattatggcg atcggtgaca
     2221 agggtatgct ggcagtagaa cattctccgg aagaagcggc taaggcagct ttaaaggcaa
     2281 tgttagatga tgaaagcgaa ctggtaacga tttactatgg ctgcgatgtt aaagaagaag
     2341 atgccgagaa actgaaggaa gaggcagaaa gtttattccc ggataaggaa ctggaacttc
     2401 agtatggcgg acagcctatc tactattata tgatttcagc agaataaact gccggttcat
     2461 atgactataa ggaggtatcg tttatgaacg aagatttcag aatggaacag ggctacaagg
     2521 aagcggctta tggagacagc cttggcagat atacagcaaa gacatt
//
LOCUS       DBMDUA010000295         2562 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_13772_length_2562_cov_2.397806, whole genome shotgun sequence.
ACCESSION   DBMDUA010000295 DBMDUA010000000
VERSION     DBMDUA010000295.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13772_length_2562_cov_2.397806"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="ACZPCL_13560"
     CDS             complement(<1..562)
                     /locus_tag="ACZPCL_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG1361 S-layer family protein"
                     /protein_id="HIR1163527.1"
                     /translation="MRNSVWKKMALLAFIWVFAAASCLSAYAGDFKLGYNTNASSNDY
                     IFTTKAPKGNIGKSMSIPFRIRATDEDMNNLRVSLLETSDFQQIESSGENDYTIDYYP
                     FEIMETTFTAKHVGNIKAGNVKSVSLSARVRRDAGQGYYSIPIQLEWDGGSDVDYINI
                     WISTSASSSADDEKKKKEGNYFVVGEN"
     gene            complement(565..1287)
                     /locus_tag="ACZPCL_13565"
     CDS             complement(565..1287)
                     /locus_tag="ACZPCL_13565"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1163528.1"
                     /translation="MNTEPPKEPSAVIRVKDLYKIYRVGETKVRALNGVSFEIPKGEF
                     VAIVGTSGSGKSTLLNMLAGLEKPTKGEILIGNVHIEKLTEKQLVAFRREKVGFIFQS
                     YNLLNTMNAIENVALPLAFRGVPKKERLKKAREYMELVGVDKQEKHMPNQMSGGQQQR
                     VGIARALVVKPQIIFADEPTGNLDSKTTMEVLQLMQKIVREQNQTLVMVTHDNNLASY
                     ADRRIRIVDGKIVNIEVGQRRE"
     gene            1645..>2562
                     /gene="pyrB"
                     /locus_tag="ACZPCL_13570"
     CDS             1645..>2562
                     /gene="pyrB"
                     /locus_tag="ACZPCL_13570"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR1163529.1"
                     /translation="MRHLLNPLDFSVEEIDDLLALARDIESNLPKYAHVCDGKKLATL
                     FYEPSTRTRLSFEAAMLNLGGNVLGFSSANSSSASKGESVADTIRMISCYADICAMRH
                     PKEGAAYVAAQKARIPVINAGDGGHQHPTQTLTDLMTIRSLKGRLDNLTIGLCGDLKF
                     GRTVHSLINAMVRYPGVKFVLISPPELRIPDSIREDVLIANNIPFEEIGSLDDSIGTL
                     DILYMTRVQKERFFNEEDYIRLKDCYILDKKRMAMAKDDMFVLHPLPRVNEISVEVDD
                     DPRAAYFRQAQYGVYVRMALIMTLLEVEKP"
BASE COUNT          639 a          605 c          535 g          783 t
ORIGIN      
        1 ggttttcgcc tactacaaaa taatttccct cttttttttt tttttcatca tctgctgagg
       61 aagaagcact ggtggagatc cagatattaa tgtaatctac atcggaaccg ccatcccact
      121 ccaactggat gggaatgctg taatagccct gtcctgcgtc acggcgtact ctggcagaaa
      181 gggaaacact ttttacattt cctgccttaa tatttcccac atgtttggca gtaaatgtag
      241 tctccatgat ctcaaacgga taatagtcaa tggtataatc attttctccg ctgctttcga
      301 tctgctggaa atcactggtc tcaagaagac tgacacgcag gttgttcata tcctcatctg
      361 tagcacggat gcggaaaggg atgctcatgg atttaccaat atttcccttt ggtgcctttg
      421 tggtgaatat gtaatcattg ctgctggcat tggtgttata ccccagctta aaatcaccgg
      481 cataggcact caggcagctg gcggcagcaa atacccatat aaaggctaaa agcgccattt
      541 ttttccatac actgtttctc attctcactc tctcctctga cccacttcta tatttacgat
      601 cttgccgtct acgatccgta tccggcggtc cgcataggaa gccaggttat tgtcatgtgt
      661 gaccataaca agggtctgat tttgctccct tacaattttt tgcatcaact gcaatacttc
      721 catggttgtt tttgagtcca ggtttcctgt aggctcgtcc gcaaagatga tctgaggctt
      781 taccactaag gctctggcaa tccccactct ctgctgctgt ccaccggaca tctggtttgg
      841 catatgcttt tcctgtttgt ctactcctac taattccatg tactctcttg cttttttcag
      901 ccgttctttc tttgggacac ctcgaaatgc caggggaagt gccacatttt ctatggcatt
      961 catggtattt aaaaggttgt aggactgaaa gataaaacct accttctccc gcctgaaagc
     1021 taccagctgt ttctctgtca atttttctat atgtacattt ccaataagga tctcaccttt
     1081 ggtgggcttt tccagtcctg caagcatatt taaaagggta gatttaccgg aacctgaggt
     1141 tcctacgata gctacaaatt caccttttgg gatctcaaaa ctgactccgt ttaatgcccg
     1201 gactttggtt tcgcctacgc ggtatatctt atagaggtct tttacgcgga tgaccgcaga
     1261 cggctctttt gggggttctg tgttcacgaa agaaaagact cctttctgcg gcagcaggcg
     1321 gaaccttcgc agccgcttgt ttttctcctt tgctcatcac attataccat agtatccccg
     1381 ttcaggctct ctgtttttcc ttgaaagttc cttaaatatt tcttactctg taattgcccg
     1441 ggtactgcac catatgcgca gggacaattt cataaaattt tacatttttc cataattgtg
     1501 cttgacttcc taggagtttt gtgtaagaat atgttgaggc acaaaatcag tgccattctg
     1561 gctaagatca gctgtcgcac tggttcagtg ctgaagtatt tcccaaaatc atgtaattga
     1621 atataaaagg caaaggagaa tcacatgaga cacttattga atcccttaga cttctctgtg
     1681 gaggagattg atgatcttct ggcgctggca agggacattg aaagcaacct tccaaaatat
     1741 gcccatgtct gcgacggcaa gaaactggct acattatttt atgagccaag tacccgtaca
     1801 cgtttaagct ttgaagctgc tatgttaaac cttggcggta atgttttagg tttttcatct
     1861 gctaattcca gttctgcatc aaagggcgaa agcgttgctg ataccattcg catgatttcc
     1921 tgctatgcag atatctgcgc tatgcgtcat ccaaaagaag gtgctgctta tgtagctgca
     1981 cagaaggcac gtattcctgt tatcaacgca ggtgacggcg gacatcagca tccaacacag
     2041 actctgaccg accttatgac catccgttcc ttaaagggtc gtctggacaa cttaaccatt
     2101 ggcctgtgcg gcgacttaaa gtttggccgt actgttcact ccctgatcaa cgctatggta
     2161 cgttatccgg gtgtaaaatt tgttctcatc tccccgcctg aacttcgtat ccctgacagc
     2221 atccgtgagg acgttctgat cgctaacaac attccatttg aagagatcgg aagcctggat
     2281 gattccatcg gtactctgga tatcctgtac atgacccgtg tacagaaaga gcgtttcttc
     2341 aacgaagaag attacatccg cttaaaagac tgctatatct tagataagaa gagaatggca
     2401 atggcaaagg atgatatgtt tgttctccat ccacttccaa gagtaaacga gatctctgta
     2461 gaagtagatg acgacccgag agctgcttac ttcagacagg cacagtacgg cgtatacgtt
     2521 cgtatggctc tgatcatgac attactggag gtagaaaagc ca
//
LOCUS       DBMDUA010000296         2551 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_13852_length_2551_cov_2.191429, whole genome shotgun sequence.
ACCESSION   DBMDUA010000296 DBMDUA010000000
VERSION     DBMDUA010000296.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13852_length_2551_cov_2.191429"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1997)
                     /gene="smc"
                     /locus_tag="ACZPCL_13575"
     CDS             complement(<1..1997)
                     /gene="smc"
                     /locus_tag="ACZPCL_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR1163530.1"
                     /translation="MYLKSIEIQGFKSFANKLLFEFHNGITGIVGPNGSGKSNVADAV
                     RWVLGEQRIKQLRGASMQDVIFSGTELRKPQGFAYVAITLDNSDHQLAIDYDQVTVSR
                     RLYRSGESEYMINGSTCRLKDINELFYDTGIGKEGYSIIGQGQIDKILSGKPEDRREL
                     FDEAAGIVKYKRRKSIAQKKLEDEQANLVRVSDILSELEKQVGPLERQSKTAREYLQL
                     KEELKVCDANQFLLDTENTHSQLKEVENRRRLLAGDVEETQKKADDLRKEYDTLEEKL
                     TALDEVLTKNRAALSEAAMEKSSREGQINVLREQIHTEEANEEHYKSRKNVISQDLEN
                     YGKQLASYVKEREDAQIQVKDAGERLEAAKTQLQEKDDQIYQLENEIEQAKSEIIQAL
                     NDKADLTARQQHYETMLEQVNLRRSEVSQKLLRFKSDESVQDEKILQEKAALDQVQEQ
                     LEKAQFSAEEAEEGMNAALKEVQRLNKNLNNTQQEYHMAHTKLESLRNLAERYDGYGN
                     SIRRVMEVRDRIHGIRGVVADIISTEKKYETAIETALGGSIQNIVTDSEETAKRLIEH
                     LKKNKYGRATFLPLTSVGKNQSPFPKPEALKEPGVLGLASSLVQADKEYEGLIRYLLG
                     RVVVADTIDHAIAIARKYHYSLRIVTLEGELCSCRTGFVCP"
     gene            complement(1988..>2551)
                     /gene="rnc"
                     /locus_tag="ACZPCL_13580"
     CDS             complement(1988..>2551)
                     /gene="rnc"
                     /locus_tag="ACZPCL_13580"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR1163531.1"
                     /translation="LEFLGDAVLEIVTSDFLYHKYTEKPEGDLTKIRASIVCEPTLAY
                     CAEAINLGSYLFLGKGEDATGGRNRNSVVSDAMEAVIGAIYLDGGFASAKEYIHRFIL
                     NDIEHKQLFYDSKTILQEIIQSRQDGELSYEILKEEGPDHNKSFEVRALVGDQEIGRG
                     KGRTKKAAEQLAAYNGILKLKAGETCI"
BASE COUNT          583 a          653 c          498 g          817 t
ORIGIN      
        1 tcaggacaga caaacccagt acgacaggaa caaagttctc cctctaaggt aacaatacgc
       61 aggctgtagt gatactttct tgcaatggca atggcatgat caatggtatc cgccactacc
      121 acccggccta aaagataccg gatcagaccc tcatactctt tgtctgcctg gactaaactg
      181 cttgcaagcc ccagtacccc cggttccttt aaagcctccg gctttgggaa cggagactgg
      241 ttctttccca cactggtaag aggcagaaaa gtagcacggc cgtatttatt cttttttaaa
      301 tgttcaatca gccgtttggc agtttcttca gagtcagtaa caatattctg gatactgccg
      361 cctaaggctg tttcaatagc tgtttcgtac tttttctctg tgctgataat atcagccaca
      421 acaccgcgga tcccatggat ccggtctctt acttccatta cccgccggat actgtttcca
      481 tagccatcat agcgctctgc caggtttcgc aaagactcta attttgtatg ggccatgtgg
      541 tattcctgct gggtgttatt taagttttta tttaaacgct gaacctcttt taaggcggcg
      601 ttcattccct cttctgcttc ttctgctgaa aactgggcct tttctaactg ttcctgtacc
      661 tggtctaaag cagccttttc ctgaaggatc ttttcatcct gtacagattc atcactttta
      721 aagcggagaa gtttctggga gacttcgctg cggcgcagat ttacctgctc taacatagtc
      781 tcataatgct gctgtcttgc agtcagatcc gccttgtcat tcaacgcctg gatgatctca
      841 cttttcgcct gttcgatctc attttccaac tgatagatct ggtcatcttt ttcctgaagc
      901 tgtgttttgg cagcttccaa ccgttctcct gcgtctttta cctgtatctg ggcatcttca
      961 cgttctttta cataggaagc cagctgtttt ccataatttt ccagatcctg ggagatcaca
     1021 tttttacggc ttttataatg ttcttcattg gcctcttctg tatggatctg ttcccttaag
     1081 acattgatct ggccttctct ggaacttttt tccatagctg cttcgcttaa agccgcccgg
     1141 ttctttgtaa ggacttcatc taaagctgtc agtttttctt ccagggtatc atattctttt
     1201 ctcagatcat ctgccttttt ctgggtctct tccacatccc cggcaagaag acgtctgcgg
     1261 ttttctacct cttttaactg ggaatgggta ttttctgtat ccagaagaaa ctgattggcg
     1321 tcacatacct ttaattcttc ttttaactga agatattccc tggcagtttt tgactgacgt
     1381 tccaaaggtc ctacctgttt ttccagttct gataaaatat cgctgacacg gaccagattt
     1441 gcctgttcat cctctaattt cttctgggca atggatttac gccgtttgta tttaacaata
     1501 ccggccgctt cgtcaaacag ctctcgtctg tcctccggct ttccgcttaa gatcttgtcg
     1561 atctggccct gtccgataat ggaataacct tccttaccga tacctgtatc ataaaaaagc
     1621 tcattaatat cctttaaacg gcaggtgctg ccattgatca tatattcact ttcaccagag
     1681 cggtacagac gtctggaaac tgtaacctga tcataatcaa tagccagctg gtgatcggaa
     1741 ttgtccagtg taatggcaac ataggcaaag ccctgaggct ttctaagctc tgtaccggaa
     1801 aagataacgt cctgcatact ggcgcctcgc agctgcttaa tacgctgttc accaagcacc
     1861 cagcggactg catcggcaac attgctcttt ccacttccgt ttgggcctac gataccggta
     1921 ataccattat gaaattcaaa aagcagtttg ttggcaaagg acttaaatcc ctggatctca
     1981 atgcttttta gatacatgtt tcacccgcct tcagttttaa gatgccgttg taggcagcta
     2041 actgttcagc agccttcttg gtacggccct ttccacggcc gatctcctga tcacctacca
     2101 gagcgcggac ttcaaaggat ttgttgtgat ccggtccttc ttctttcagg atctcgtagc
     2161 ttaattcgcc gtcctggcgg ctctgtatga tctcctgtaa gatagtcttg ctatcataaa
     2221 agagctgctt gtgttcaata tcatttaaaa tgaacctgtg aatatactct tttgcactag
     2281 caaaaccacc gtccagataa atagctccga tcactgcttc catagcatct gatacaacag
     2341 aatttctgtt tcttccgccg gttgcgtctt cgcctttgcc taaaaacaga tagcttccaa
     2401 ggttgatagc ttctgcacag tacgccaggg tcggctcgca gacaatgctg gctctgatct
     2461 ttgtcagatc accttccggc ttttctgtat atttgtgata gagaaaatca ctggtaacga
     2521 tctcaaggac tgcatctcct aaaaattcca g
//
LOCUS       DBMDUA010000297         2525 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_14050_length_2525_cov_3.356848, whole genome shotgun sequence.
ACCESSION   DBMDUA010000297 DBMDUA010000000
VERSION     DBMDUA010000297.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14050_length_2525_cov_3.356848"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            73..756
                     /locus_tag="ACZPCL_13585"
     CDS             73..756
                     /locus_tag="ACZPCL_13585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1163532.1"
                     /translation="MYLGRNGSGKTTFYNVLTGISPATRGQIIFKGKSIEKMQPEAIT
                     RLGMARTFQNIRLFGNMTVAENIIIARHCRRKTGIWDSLIGSPRLKKEEAENQEYIKK
                     CLEYVGIYDKKDWLAKNLPYGMQRRLEIARVLATEPSLILLDEPAAGMNPHEKDELVE
                     IINRLKAEKYSIILIEHAMRMVMNIADRVVVFDHGQKIAEGLPQDVKNDPVVIESYLG
                     KGASANGDA"
     gene            743..1450
                     /locus_tag="ACZPCL_13590"
     CDS             743..1450
                     /locus_tag="ACZPCL_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1163533.1"
                     /translation="MAMLDVKDLNVYYGKIHAIKNISLTVEEGELVSLIGANGAGKTS
                     LLTAIMGQIPSTGQISFEGQAVHGNGTMEIVRNGMTMVPEGRRVFPRTSVMDNLLLGA
                     YSRKDKENIKGDLNKIFEMFPRLLERKNQLAGTLSGGEQQMLAMGRALMSRPRLLLLD
                     EPSMGLSPRLVDQVFDTIVSINKSGTTIMLVEQNANQALRVAKRGYVIETGEIVLSDD
                     ADKLLSNPQVKEAYLGG"
     gene            1536..2519
                     /locus_tag="ACZPCL_13595"
     CDS             1536..2519
                     /locus_tag="ACZPCL_13595"
                     /inference="COORDINATES: protein motif:HMM:NF012607.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1163534.1"
                     /translation="MKVLVWCATAEDCRVFEQYNKGRIEFEYTPDTLTEENVSSCKGY
                     EGIVIQTRCMITEKVAALLAEAGVIFIACRSAGFDHVNLAAIKKYGIKACNVPLYSPN
                     AIAEYAVMASMMALRKVKKQLHMTEKGDYTLKGLRGQELRDQTIGIIGTGRIGMESLK
                     IFSSFTKDILLYDPYPREDAKAMGIYVSLDEIYEKSTLLVFHCPFTKENFHMVNGDSI
                     RKMRTGVVLVNPARGGLFDHQAVLEGLKEGKIGALAMDVYEDEKNYLRKDLKGESTGD
                     AVFDQLISREDVIYTAHTAFYTDRAIFNMIETTVENLWEYGQTGSCAHELT"
BASE COUNT          797 a          485 c          642 g          601 t
ORIGIN      
        1 agatattccc agataaggtt atagttatag taccgatatt gagcgagggg atagtagtag
       61 agaaaactca gtatgtatct agggagaaat ggttcaggaa agactacatt ttataatgta
      121 cttacaggaa tttcccctgc aaccagaggc cagatcattt tcaagggcaa aagcatagaa
      181 aaaatgcagc cggaagctat tacccgcctt ggcatggccc gtactttcca gaatatccgt
      241 ctttttggaa atatgacagt agcggaaaat atcattattg cccgtcactg cagaaggaag
      301 accggtatct gggactcttt aataggaagt ccaagactta aaaaagaaga ggcggaaaat
      361 caggaatata ttaagaaatg cctggaatac gtaggcattt atgataaaaa agactggctg
      421 gctaaaaacc ttccatacgg catgcagcgc cgactggaga tcgccagagt tctggcaaca
      481 gagccaagcc tgatcctact tgatgagccg gcagcaggca tgaacccaca tgaaaaggat
      541 gagctggtgg agatcatcaa tcgcctgaaa gcagagaaat attccattat cctcattgaa
      601 catgccatgc gtatggtcat gaatattgca gaccgggtgg tagtatttga ccacggacag
      661 aagatcgcag aaggactgcc acaggatgta aaaaatgatc ctgttgtgat tgagtcttat
      721 ctgggaaagg gggcatctgc aaatggcgat gcttgatgta aaagacctga atgtatatta
      781 tggcaagatc catgctatta aaaatataag tcttacagta gaagaaggag agctggtctc
      841 cctgatcggg gcaaacggcg caggaaagac ctctcttttg acggctatta tgggccagat
      901 cccgtctaca ggccagatca gctttgaagg ccaggcagtc cacggaaacg gaaccatgga
      961 gatagtaaga aacggtatga ccatggtacc ggaaggcaga cgtgtatttc caagaacttc
     1021 tgtaatggac aatctgctgt taggcgctta cagccgtaag gataaggaaa atataaaagg
     1081 cgacttaaac aagatctttg aaatgttccc aagactgtta gaacgtaaaa accagctggc
     1141 aggcacctta tccggcggcg aacagcagat gctggctatg gggcgcgcac tgatgtcaag
     1201 accaagactt ctgctccttg atgagccgtc tatgggactt tcaccaagac tggttgacca
     1261 ggtatttgat accattgttt ctattaataa gtcaggaacc accattatgc tggtagagca
     1321 gaacgcaaac caggctcttc gtgtggcaaa aagaggctat gtaatagaga cgggtgagat
     1381 cgttctttcg gatgatgcag ataagttatt gtccaacccg caggtgaagg aagcatatct
     1441 gggtggttaa aacacgggag taattggaaa aatctggaaa ataccacttg aaaattgcaa
     1501 tctgggaaat aggatctgga aaggcgggag caaaggtgaa agtactggta tggtgtgcta
     1561 ctgcagagga ctgcagggta tttgaacagt ataataaggg aagaattgag tttgaatata
     1621 ctccggatac attaacagag gaaaatgttt cctcctgtaa aggctatgaa gggatcgtga
     1681 tccagaccag gtgcatgatc acggaaaagg tagcagccct tttggcagag gcaggagtaa
     1741 tatttatcgc ctgccgttcc gccggttttg accatgtaaa cttggcagcc ataaagaaat
     1801 atggcatcaa ggcctgcaat gttcctttgt attctccaaa tgccattgcg gaatacgccg
     1861 tcatggcatc tatgatggcg ctgcggaagg taaaaaagca gctgcacatg acagaaaaag
     1921 gagattatac cttaaaaggt ctgcgcggcc aggaactgcg tgaccagacc attggtatta
     1981 tcggaaccgg ccggatcggc atggaaagtc tgaaaatatt ttcctctttc acaaaggata
     2041 ttttactcta cgacccttat ccaagagaag acgcgaaagc aatgggaatc tatgtttctc
     2101 tggatgagat ctacgaaaag tcaacactct tagtatttca ctgtcctttt acaaaagaaa
     2161 atttccatat ggtaaatggt gacagcatcc gtaaaatgcg cacaggtgtg gtccttgtaa
     2221 acccggcaag agggggactt tttgatcatc aggctgtatt agagggctta aaagaaggaa
     2281 agatcggtgc ccttgcaatg gacgtttacg aggatgaaaa aaattatctg cgtaaggacc
     2341 tgaaagggga aagtacaggt gatgctgtat ttgaccagct tatttcaaga gaagatgtga
     2401 tctatactgc ccatactgct ttttatacag accgtgcgat ctttaatatg atcgagacca
     2461 cagtggaaaa tctctgggaa tacggacaga caggaagctg tgctcatgaa ctgacctaag
     2521 agagc
//
LOCUS       DBMDUA010000298         2507 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_14196_length_2507_cov_3.232336, whole genome shotgun sequence.
ACCESSION   DBMDUA010000298 DBMDUA010000000
VERSION     DBMDUA010000298.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14196_length_2507_cov_3.232336"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            85..585
                     /locus_tag="ACZPCL_13600"
     CDS             85..585
                     /locus_tag="ACZPCL_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009303 - rRNA transcription [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR1163535.1"
                     /translation="MYEKGLYIVYGIRGVCQVADIITIDHPVGPKGRLYYELHPYNQK
                     DGKIVTPVDSEKTITRLLLTRPEAEALIQEIPQIKEMTVANDKQREERYKEALKTCDC
                     RVWVSMIKALYLRKQDRIEHKKKVTDLDERYFRMAEDYLNSELSLALDISKEKINEYI
                     KERIVV"
     gene            complement(660..1589)
                     /gene="arcC"
                     /locus_tag="ACZPCL_13605"
     CDS             complement(660..1589)
                     /gene="arcC"
                     /locus_tag="ACZPCL_13605"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - L-arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIR1163536.1"
                     /translation="MAKKRIVMAIGHKDMGTNLPEQKEAVKRTAKVIADFIQDGWQVA
                     IVHSNAPQVGMIHTAMNEFGKQHDGYSSAPMSVCSAMSQGYIGYDLQNGIRAELIKRG
                     IYKPVSTVLTQVTVDPYDEAFYTPVKVVGRVMSEEEAKEEEAKGNHVKAVEGGFRRIV
                     ASPHPVAIVEIDAIKALMDADQIVIACGGGGIPVMEQGYNLRGASAIIEKDLATGLLA
                     KEIDADVMMILTSVDNVTLNFGTDKEEPIHHMTIDEAKAYMDQGQFEFASMLPKVSAS
                     VNFLEAGKGRKAIITSLDKAEESLTGEAGTTIE"
     gene            1748..2209
                     /locus_tag="ACZPCL_13610"
     CDS             1748..2209
                     /locus_tag="ACZPCL_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HIR1163537.1"
                     /translation="MRILPEGFGWKVLAAMAAAVAGNILVQYVIPWPDFMKASYGAAA
                     EILGDYPVGVYLLLTLLIAPLVEEGIFRRGIYAVFRRWLGVTPAAFLSALAFGLYHGN
                     WIQGIYGFVFGLLLAWGYESSSFGKYRMVVLMHGAANGAAVLCSMVFSGLF"
     gene            2267..>2507
                     /locus_tag="ACZPCL_13615"
     CDS             2267..>2507
                     /locus_tag="ACZPCL_13615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1163538.1"
                     /translation="MKLLSIAIPCYNSEAYMEHCINTLLTGGDEVEIIIVDDGSQKDR
                     TPEIADEYERRYPGICRAIHQENGGHGQAVNTGLKN"
BASE COUNT          657 a          490 c          659 g          701 t
ORIGIN      
        1 ctaaactgaa acaggaaact gtgaatgtga agcatgagaa aatctgggaa agtctcagat
       61 acaaaagaaa gcaggcaaaa ttttatgtac gagaaaggac tatatattgt atacggcatc
      121 cgtggagtct gccaggtagc agacattatc accattgatc atcccgtcgg gcccaaaggg
      181 cgtctttatt atgaacttca tccttataat cagaaggatg gaaagatcgt cacaccggta
      241 gactcggaga agaccattac aaggctgttg cttaccagac cggaggcaga ggcgctgatc
      301 caagagatcc ctcagatcaa agaaatgacc gtggcaaatg acaaacagag ggaagaacgg
      361 tataaagagg ctttaaaaac ctgcgactgc cgtgtatggg tgagtatgat caaggcgttg
      421 tatctgcgaa aacaggaccg gatcgagcac aagaaaaagg tgacggatct ggatgagcgg
      481 tattttcgca tggcagaaga ttatctgaat tcagagcttt cccttgcact tgatatatct
      541 aaggaaaaga taaatgaata tattaaagaa cggattgtgg tgtgaaagcc acggtccttt
      601 tttactttat aggaaagacc gcctgcgtta gcaggcggca atttttatat tcggatgatt
      661 tattcgatgg tggttccggc ttcgccagtc agactttctt ctgccttgtc cagagaagtg
      721 atgatcgcct tacggccttt tcctgcctct aagaagttta cagaagcgga aacctttggc
      781 agcatggaag caaattcaaa ctgtccctgg tccatatatg cctttgcttc gtcaatggtc
      841 atatgatgga tcggctcttc tttatcagtt ccgaagttta aggtaacatt gtcaacgctt
      901 gtaaggatca tcataacgtc agcgtcgatc tcttttgcta aaaggccggt tgccaggtct
      961 ttttcaataa tggcactggc gcctcttaag ttatatccct gttccataac cgggatgccg
     1021 ccgcctccgc aggcgatgac gatctggtct gcgtccatta aagccttgat ggcgtcgatc
     1081 tcaacaatgg caactggatg aggagaagct acgatacgtc tgaagccgcc ttctacggct
     1141 tttacatggt tgccctttgc ctcttcttct tttgcttctt cttcgctcat tacacggcca
     1201 actactttta caggggtgta aaatgcctca tcgtaaggat ctacggttac ctgggtaaga
     1261 acggtgctta ccggcttgta gatgccacgt ttgatcagtt cagcgcggat gccgttctgt
     1321 aagtcatagc cgatatagcc ctggctcata gcggagcata cagacatagg agcggagctg
     1381 tatccgtcgt gctgcttgcc gaattcattc attgctgtgt ggatcattcc tacctgtggt
     1441 gcgttgctgt gaacgatggc aacctgccag ccgtcctgga tgaagtctgc gatcactttt
     1501 gcagtgcgct ttacggcttc cttctgttcc ggaagattgg ttcccatatc tttatggccg
     1561 atggccatga cgatcctctt ttttgccata atgcttacct cacttttcag gaaatatttt
     1621 taacattatg atacattatt atgatgataa ttgcaatgaa aagcgaaaaa agatgatagc
     1681 aaaaggttga tggctgtgct atactgactg catgtgtgta aggcggaaaa aagagaatgt
     1741 gaatagaatg aggatcttgc cggaaggctt cggatggaag gttctggcag ccatggcagc
     1801 agctgtggct gggaatatcc tggtgcagta cgtgatcccg tggccggatt ttatgaaggc
     1861 gtcttatggg gctgcagcag agatactggg ggattatcct gtgggagttt atctgctttt
     1921 gacgcttctt atagcgcctc tggtggaaga agggatattt cgcagaggga tttatgcagt
     1981 ctttagaaga tggctgggag tgactccggc tgcgttttta tcagcacttg catttggtct
     2041 gtatcatggg aactggatcc agggaattta tggttttgtt tttgggctgc ttcttgcctg
     2101 gggatatgag agcagttctt ttggaaagta caggatggtt gtgctgatgc atggggctgc
     2161 caatggggcg gcggtgcttt gcagtatggt ttttagtggc ttattttaag taatgatgaa
     2221 gtgtagataa cagcaaaata agaaagagaa gcgaaggagg aagaacgtga agttattatc
     2281 cattgcgatt ccatgttata attcagaagc ttatatggaa cattgtatca atacactgct
     2341 tacaggtggt gatgaggtgg agatcattat tgtagatgat ggttcacaga aggatcgtac
     2401 accggaaatt gccgatgaat atgagcggag atacccgggg atctgtcgtg caatccatca
     2461 ggaaaatggt ggacatggcc aggcggtaaa tacaggtctt aaaaatg
//
LOCUS       DBMDUA010000299         2477 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_14423_length_2477_cov_3.145623, whole genome shotgun sequence.
ACCESSION   DBMDUA010000299 DBMDUA010000000
VERSION     DBMDUA010000299.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14423_length_2477_cov_3.145623"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="ACZPCL_13620"
     CDS             <1..271
                     /locus_tag="ACZPCL_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1163539.1"
                     /translation="MPLDKALVEAGAIRMRPILMTTGTTVISMLPNAVAYGKSGAMMQ
                     GLALVDVGGLIAATILTLILLPTYYRLVHNMGRKAVGEDVPMVSD"
     gene            451..957
                     /locus_tag="ACZPCL_13625"
     CDS             451..957
                     /locus_tag="ACZPCL_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5721 family protein"
                     /protein_id="HIR1163540.1"
                     /translation="MIALKAEDVKSFTTKLFVREDFDAFLVKEVNITTYNSFSIDGHV
                     KQGYYTEEEREENNIEEFSTWKTLRPFCFSLIKGKKLPGSFRIVLQLPKAGTDKFAAR
                     TGAGIDGNQIVGLYLNIRYDNGEMYCITGTSLNFFTMDKTLDLEWDKAVKQFLQSHEI
                     PCTEATEG"
     gene            975..2189
                     /locus_tag="ACZPCL_13630"
     CDS             975..2189
                     /locus_tag="ACZPCL_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1163541.1"
                     /translation="MDKNNFSQKLAELTALAAVSENKLQMTQVLDAFAKEQVTPEEME
                     SVYAKLEKKGIQILTEGEEGDLADESLLLDDGDDSDDSVSSIIGDKYRWSHNDSLDDG
                     DNDEFTSSSADDEEGKNATQEQLLEGVASTDPIREYLKEIGSIPLLTQEQEQDLAKRK
                     SEGDAEAGKKLVEANLRLVVSIAKRYTGRGMSFLDLVQEGNIGLMKAVEKFDYTKGYR
                     LSTYATWWVKQSVTRALADQSRTIRLPVHMVEAVNRIRKAQRALAVKLGREPSNEEIG
                     KEVGMSEKRVTELMQSSGDTVSLETPVGDEDGSNLGDFVADDSNASTEEKAESVFLRE
                     EIEQMLQGLNPREREVIILRFGLESGHPLTLEEVGKRFKVTRERIRQIETAALRKLRN
                     PSRSKKIRDFLP"
BASE COUNT          773 a          444 c          623 g          637 t
ORIGIN      
        1 aatgcctctg gataaggcgc tggtagaagc tggtgctatc cgtatgagac ctattttgat
       61 gacaacagga acaaccgtga tctccatgct tcctaacgca gttgcttacg gtaaatccgg
      121 tgccatgatg cagggccttg cactggtaga tgtaggtggt ctgatcgcag caacgatcct
      181 gacactgatc cttcttccaa cttattacag actggtccac aatatgggac gtaaggcagt
      241 tggggaagat gtgcctatgg tttctgatta aaaattaaga taagtaagat aataagagag
      301 caggactttt gtggttttgc tctcttttgg cgttgttaca gttcagccat gacatggcgt
      361 tatgcttttt gtataaacct gattgacgtt ttgcctgaaa aattgtataa ttgataaaga
      421 atttggaaat tttcagacgg gagaatacaa atgatcgcat taaaggccga agatgtaaag
      481 tcctttacta ccaaactgtt tgtacgggag gattttgatg catttctggt aaaagaagtg
      541 aacattacca cctacaacag tttttccatt gacggacatg taaaacaggg ctattacaca
      601 gaggaagaac gggaagaaaa caacatagaa gagttttcaa cctggaaaac cttaaggcct
      661 ttttgttttt cactgatcaa aggaaaaaag cttcctggaa gttttcgcat cgtgctgcag
      721 ctgccaaagg cggggacaga taaatttgcc gcaagaacag gcgccgggat cgatggaaac
      781 cagatcgtag ggttatatct gaatatacgt tacgataatg gggaaatgta ttgtattacc
      841 ggaacatcat taaatttttt caccatggac aaaacgctgg atcttgaatg ggacaaggcg
      901 gtcaaacagt ttttacagtc ccatgagatt ccatgtacag aggcaactga aggataaaag
      961 caggaggaaa tggcatggat aaaaacaatt tttcacagaa attggcggaa cttacagctc
     1021 tggcagcggt cagtgagaac aaactccaga tgacacaggt tctggacgct tttgctaaag
     1081 aacaggtgac accggaagaa atggaatctg tttatgccaa gctggaaaaa aaggggatcc
     1141 agatactgac agagggggaa gaaggggatt tagcagatga aagtcttctt ttagatgacg
     1201 gggatgattc ggatgattct gtttccagca tcattggaga taaatacagg tggagtcaca
     1261 acgattcttt agatgacggg gacaatgatg agtttacgtc ttcttctgcc gatgatgaag
     1321 aagggaaaaa tgctacccag gagcagttat tagagggcgt tgcaagtaca gatcccattc
     1381 gtgaatattt aaaagagatc ggttctatcc ccctgttgac ccaggaacag gaacaggatc
     1441 tggcaaagcg taaatccgag ggagatgcag aagcaggaaa gaagttagta gaagcaaacc
     1501 ttcgtctggt agtcagcatt gcaaagcgct ataccggtcg tggtatgagt tttctggatc
     1561 tggtccagga gggaaatatc ggtcttatga aggctgtaga gaagtttgat tatacaaagg
     1621 gataccgtct cagtacctat gccacatggt gggtaaaaca gtctgttaca agagctttgg
     1681 cagaccagtc ccgtaccatc cgcctgccgg tccatatggt agaagcggta aaccgcatca
     1741 gaaaagccca gagagcttta gctgtaaagc ttggaagaga gccttccaat gaagaaatcg
     1801 gcaaagaagt gggaatgtct gaaaagcggg ttacagagct gatgcagtct tccggtgata
     1861 cggtatcctt agagacaccg gtaggagatg aagacggctc aaatttaggc gattttgtgg
     1921 cagatgattc caatgcttcc acagaggaaa aagcagaaag cgtcttttta agagaagaga
     1981 tcgaacagat gctccagggg ttgaatccaa gggaaaggga agtgatcatc ttgagatttg
     2041 gtcttgaaag cggccatccc ctgacactgg aagaagtagg aaagcgcttc aaagttacca
     2101 gagagcgtat caggcagatt gagacagcag cgcttagaaa gcttcgcaat ccgtccagaa
     2161 gtaagaaaat cagggatttt ctgccgtgaa aaatttacag aaaggtcata tcaggtgact
     2221 cacggatcca cagttaatac ttttttgctt gatccactgt acaataaggt acggtggatc
     2281 tttttatgta aaaatggtaa tgtgaacagc aggtgatgta cttttgctag cactcattta
     2341 aaatgagtgc taatttttgt tgacttttag atataacggt actacaatat atcttgcgga
     2401 caaaatccgt ttttaagatt ttcaaagcac atattactag tacatcgttt cgtaaataac
     2461 tgtactaatc acgtagg
//
LOCUS       DBMDUA010000300         2471 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_14464_length_2471_cov_2.291983, whole genome shotgun sequence.
ACCESSION   DBMDUA010000300 DBMDUA010000000
VERSION     DBMDUA010000300.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14464_length_2471_cov_2.291983"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(190..1122)
                     /locus_tag="ACZPCL_13635"
     CDS             complement(190..1122)
                     /locus_tag="ACZPCL_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HIR1163542.1"
                     /translation="MSAEVETMFYTRVAPWHGLGTCVENAPGSQEALKVAGLDWKVVQ
                     KPIFTEEGQLVGGFKANIRDRDNQILGVVTDRYKVVQNEEAFAFTDQLLGEGITYETA
                     GSLQGGRRTWILAKLPQRYIISGDEITPYLVFMNSHDGTGAIKAAMTPIRVVCQNTLN
                     LALSNAKRSWSANHVGNINGKLDDARYTLLYADKYMAELGRSIDRLNQIRLSDQKVYE
                     YINALFPLADNATEQQKKNLLRMKEDVKARYFEAPDLKHIGKNGYRFVNAVSDFATHS
                     KPLRERSTYRENLFSRTIEGNPMIDRAYSLLQTA"
     gene            1362..2231
                     /locus_tag="ACZPCL_13640"
     CDS             1362..2231
                     /locus_tag="ACZPCL_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HIR1163543.1"
                     /translation="MEPLKTNWESIGLSNDFLFGKIMRKPALCKRMLEIILGIKIDRI
                     EYLESQKSIDEEWDARSIRLDIYVQDNKDIAYNIEIQTTNTGDLPKRSRYYQSVLDMQ
                     QLNKGERYRNLKRTYIIFICTFDLFKLGRHVYTFENQCCEERSLQLGDEAYKLFLNTE
                     GIMDDVSTDLKAFLDYMGGRPSNHEFIKELSHEVALAKQNKEWRREYMTLLMRDQENI
                     EKGREEGLKEGLKKGLKEGLKEGLREGQQKGESLFAELTRQLLKDKRYNDLEKGTIDT
                     EYRKKLYEEYHIL"
BASE COUNT          757 a          525 c          515 g          674 t
ORIGIN      
        1 gcagaccgtt ccagctcaat ttgcataacc tcacctcttt ttcaaacact gtttccatta
       61 taacagactc ttatacaaat tcctatggaa atcttcctgt atcatgcctc ctctgcctta
      121 tcttaaaaag atctcaataa taatcttaat cagacgcatt ttctcacctc ctctccctgt
      181 gcagctgatc tatgccgtct ggagaagaga gtatgcccgg tcgatcatag gattcccctc
      241 aatggtacgg gaaaacaggt tttcgcgata agtgctccgc tccctcaatg gtttggaatg
      301 cgtagcaaaa tcagatactg catttacaaa acgataacca ttctttccaa tatgctttaa
      361 atcaggtgcc tcaaaatacc gggcttttac gtcctccttc atgcggagca ggttcttttt
      421 ctgctgctct gtggcgttgt ctgccagagg gaacagggca ttgatgtact catagacctt
      481 ttgatcggaa agccggatct gatttaatcg gtctatgctg cgcccaagct ctgccatata
      541 cttatctgca tacagcagcg tataacgggc gtcatccaac ttcccgttaa tattccctac
      601 atgattggca gaccagctcc gttttgcatt tgaaagagcc agattcagcg tgttttggca
      661 tactacacgg attggtgtca tggctgcctt aattgctccc gttccatcat gtgagttcat
      721 aaatacaaga taaggcgtga tctcgtcccc gctgataata taacgctgtg gcagctttgc
      781 cagtatccat gttcttctgc ctccctgcag agatcctgca gtttcatagg tgatgccctc
      841 acccaacagc tggtctgtaa atgcaaaggc ttcctcattc tgtacaacct tatagcggtc
      901 tgttactact cccagaatct ggttatccct gtcccggata ttggctttaa agccaccaac
      961 cagttgtcct tcctctgtaa aaatcggctt ctgtactact ttccagtcaa gcccagctac
     1021 cttcaaagcc tcctgcgatc ccggtgcatt ctccacacag gttcccaaac cgtgccatgg
     1081 ggctaccctc gtgtaaaaca tagtttcaac ttctgctgac ataaattata cctccttaaa
     1141 tgtggtctgt actcattaaa agtacattgt ttgattactt gattccggag tataatatat
     1201 atgtaaaata agcagtcaga cggattttag cggttttggc tgggagccgc cttgttcctg
     1261 attttaaaat atgatatgat aaaagtgaat caaataattg ctgtcacaca gtaaaaccgt
     1321 caacaaatag gacgtataat cactgtaaaa atgaggtatg tatggaaccg cttaaaacaa
     1381 actgggaaag tatcggttta tccaatgact ttctttttgg aaaaattatg agaaagccag
     1441 cgttatgcaa acggatgctg gaaattatat tgggaataaa aattgaccgg attgaatatc
     1501 tggaatccca gaaaagcata gatgaagaat gggatgccag aagcatccgt cttgacattt
     1561 atgtgcagga taataaggat attgcttata atattgagat acagacaaca aataccgggg
     1621 atctgcctaa gcggagccgg tactatcaat ccgtattaga tatgcagcag ctgaataagg
     1681 gagagcgcta ccggaatctg aaacgaacct atatcatctt tatctgcaca tttgatctct
     1741 ttaaacttgg acggcatgta tacacctttg aaaatcagtg ctgcgaagag cgttctctgc
     1801 agttaggtga tgaggcctat aagctgttcc tgaatacaga aggaatcatg gatgatgtaa
     1861 gcactgactt aaaagccttt ctggactata tgggaggccg tccatccaac catgaattta
     1921 taaaagaact aagccatgaa gtagctttag cgaagcagaa caaagagtgg aggcgagaat
     1981 atatgacatt gttaatgcgc gatcaggaaa atatcgaaaa aggacgggaa gaaggcttaa
     2041 aagaaggctt aaagaaaggc ttaaaagaag gtttaaagga aggcttaagg gaaggacaac
     2101 agaaaggtga atccctcttt gccgaactca cccgacagct tctcaaagat aagcggtata
     2161 atgacttgga aaaaggaacc attgacacag aatatcggaa aaagctgtat gaggagtacc
     2221 atattttata acctttttcc ttaaaaaaca gctatacgat tgaaaatctt tgaaatgtgt
     2281 acgcactgac ttaaaagcct ttctggacta tatgggaggt cgtccgccaa accatgaatt
     2341 tataaaagaa ctaagccatg aagtagcttt agcgaagcag cataaagagt agaggcgaga
     2401 atatatgaca ttgttaatgc gcgatcagga aaatatcaga aaagatacca agagggcata
     2461 agagaaacag a
//
LOCUS       DBMDUA010000301         2374 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_15280_length_2374_cov_1.758469, whole genome shotgun sequence.
ACCESSION   DBMDUA010000301 DBMDUA010000000
VERSION     DBMDUA010000301.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15280_length_2374_cov_1.758469"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(83..1384)
                     /locus_tag="ACZPCL_13645"
     CDS             complement(83..1384)
                     /locus_tag="ACZPCL_13645"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HIR1163544.1"
                     /translation="MYENLLKLIQRAETADRIGKIENVVGMSMEASGGRSGVGDIVMI
                     YNEAENCQVPAEVVGFKNGKIQLMTYENISGITAGSFVRNTGSRLQIPVGDFLKGRII
                     NALGHPIDGKGEFPPGKLYTVDSAYINPLNRPPIREHLEFGIKAIDGLNTIGKGQRMG
                     IFAGSGVGKSTLMGMIARNVKTDINVIALVGERGREVLEFVKKDLGEEGMKRSVLIVA
                     TSDQPAMLRMKCPLVATTIAEYFRDQGADVLLMMDSLTRYAMAQREIGLAIGEPPIAR
                     GYTPSIYAEFPRLLERSGNFAKGSITGIYTVLVEGDDTNEPVADTVRGILDGHIVLSR
                     QLANANHFPAIDISASISRLMNDIAGEEHKKAASRFRNMLSLYQKNADLISIGAYKKG
                     ANSALDEAVDRIGDMNKFLEQETNENIPYEESIRRMKKIVG"
     gene            complement(1424..2251)
                     /locus_tag="ACZPCL_13650"
     CDS             complement(1424..2251)
                     /locus_tag="ACZPCL_13650"
                     /inference="COORDINATES: protein motif:HMM:NF014196.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HIR1163545.1"
                     /translation="MTITLLSDQDTGFENKARVRELPLQVFTAPDAQELETERQNKRE
                     TEASLNAGNDTYNGQECSQEQIELVLREKEKILEEARKEAAAIEQEANEKAEQIFKEA
                     QMNGFEEGRQKGYEEGLIRAEEENEAKHHENQRFFKESLEKALTEISKEKEKCLREYL
                     EELKDIAVAVAEKVVHISLRSSGSVISRMIEAETEKLQKTDWVKIYMEKEDYETMVQA
                     DGHLAEKLSRLSDNVKFIVMEDGKCGSCIIEMPDGIIDMSVDTQLENIHRLVENTNT"
     gene            complement(2229..>2374)
                     /locus_tag="ACZPCL_13655"
     CDS             complement(2229..>2374)
                     /locus_tag="ACZPCL_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HIR1163546.1"
                     /translation="GDLEITTNVRLKEVEEAQQRIVNLIRKLEDAGEVIINKGGDDDN
                     IVV"
BASE COUNT          532 a          625 c          394 g          823 t
ORIGIN      
        1 ccagaatctg ttccttataa tgaagtacat tttcaagact gtatttaaat ctcttcatct
       61 taagtatcat ctcctataac tgctaaccca cgattttttt cattctcctt atgctttcct
      121 cataagggat attttcattt gtttcctgtt ccagaaattt attcatatcc cctatacggt
      181 caactgcttc atccagagca ctgtttgcac cttttttgta agcaccaatg gagatcaggt
      241 ctgcattttt ctgataaaga cttaacatgt tcctgaacct tgaagctgct ttcttgtgtt
      301 cttccccggc tatatcattc ataagacgtg aaatacttgc actgatatca atagccggaa
      361 aatggtttgc attagccagc tgcctgctta aaacaatatg accatccaaa atacctctga
      421 ctgtatcagc caccggctca ttggtatcgt ctccctccac cagtaccgta taaatcccgg
      481 taatggaacc ttttgcaaaa ttcccgcttc tttccagaag ccttggaaat tctgcataaa
      541 tagaaggtgt ataacctctg gctataggcg gttctccaat agcaagaccg atctccctct
      601 gtgccatggc atatctggta agagaatcca tcatcagcag cacatctgct ccctgatccc
      661 tgaaatattc agcgatcgtt gttgcaacca agggacattt catacgcagc attgccggct
      721 ggtctgatgt ggctacaata aggacagaac gtttcatgcc ctcttctccc aggtcctttt
      781 taacaaattc cagaacctcc ctgcctcgct ctcctaccag agcgatcaca ttgatatctg
      841 tttttacatt ccgggcgatc attcccatca atgtactttt tccaacaccg gagcctgcaa
      901 aaatccccat tctctgacct tttccaatgg tatttaaacc atcaatggcc ttaatcccaa
      961 attccagatg ttccctgata ggaggacggt taagagggtt gatataggca ctgtctactg
     1021 tataaagctt ccccggcgga aactctcctt ttccgtcaat aggatgtccc agcgcgttga
     1081 tgatgcgccc tttcaaaaaa tctcctactg gtatctgaag acggcttcct gtattgcgga
     1141 caaagcttcc cgctgtgatc ccgctgatat tttcataggt catcagctgt attttgccat
     1201 ttttgaatcc taccacttcc gcaggaacct gacagttctc agcttcattg tagatcataa
     1261 caatatcacc aacaccagat ctgcctccgg aagcttccat tgacattccc actacatttt
     1321 caatttttcc aatgcggtct gctgtttctg cccgctgtat taacttcagg agattttcat
     1381 acatggtttt cacctctgtg tactttatgt atccataaaa tctttatgta ttggtgtttt
     1441 ctacaagtct gtgtatattt tcaagctggg tatctacact catatctatg atcccgtccg
     1501 gcatttcgat gatacagctt ccgcattttc cgtcctccat tactataaat ttgacgttgt
     1561 cagagagccg tgacagtttc tctgccagat gtccatctgc ctgaaccatg gtctcatagt
     1621 cttctttttc catatagatc ttgacccagt ccgttttctg cagtttttct gtttcagctt
     1681 ctatcatcct gcttataaca gaaccgctgg atctcaggct gatatggacc actttttcag
     1741 ccactgccac agcaatatct tttaattctt ccagatattc cctgaggcat ttttcttttt
     1801 ctttgctgat ctctgttaag gctttttcca agctttcttt gaaaaaccgc tgattttcat
     1861 gatgtttcgc ctcattttcc tcttctgccc ggatcagtcc ctcttcatag cctttttgtc
     1921 tgccttcttc aaagccgttc atctgggctt ccttaaagat ctgttctgcc ttttcgtttg
     1981 cttcctgttc aatggctgcc gcttcttttc gggcttcttc taatattttt tctttttccc
     2041 tgagcaccag ctctatttgt tcctgactgc attcctgacc attgtatgta tcattgcctg
     2101 cgtttaaaga agcttccgtt tctcttttat tctgcctttc tgtttctagt tcctgtgcat
     2161 ccggtgctgt aaatacctgt aagggaagct cccggactct ggctttattt tcaaagcctg
     2221 tatcctgatc agacaacaat gttatcgtca tctcctccct tattgattat tacttctcct
     2281 gcatcttcca gtttacggat cagattaaca atacgctgct gtgcctcttc cacttcctta
     2341 agccttacat tggtagtgat ctccaggtct ccct
//
LOCUS       DBMDUA010000302         2361 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_15391_length_2361_cov_3.320796, whole genome shotgun sequence.
ACCESSION   DBMDUA010000302 DBMDUA010000000
VERSION     DBMDUA010000302.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15391_length_2361_cov_3.320796"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2246)
                     /gene="priA"
                     /locus_tag="ACZPCL_13660"
     CDS             complement(<1..2246)
                     /gene="priA"
                     /locus_tag="ACZPCL_13660"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR1163547.1"
                     /translation="MNYNTDNKTDTIYQPTIYQYANIIIDISHESVDKTFQYRIPEHL
                     LGQITAGQQVYIPFGAGSHKRKGYVVELTNQAEYDISKIKKIDSLVEGSITAQSQLIH
                     LAWWMKERYGSTMNQALKTVLPVKQKVREAPKRKIHLLVDALTLEAAIQTAERKKYKA
                     RLRLLYALKENPDLPYENTLHKLNLTAAAIRPLEQEGLLAVQVVNQYRNPLEEMRRLL
                     TDASKAQWKPPVLNKDQQRIAANICEKYDNGDRKPCLIHGITGSGKTEVYMELISHVI
                     RAGKQVILLIPEISLTWQTVMRFYLRFGDRVSVLNSRMSAGERFDQYERARTGDIDII
                     IGPRSALFVPFERLGMLIIDEEHENSYKSELSPRYDAREVARKRGEMEGAMLVLGSAT
                     PSLETYTRAVRGEYDLYKLTRRAKADAVLPTVEIADLREELKAGNRSIFSARLKELLE
                     DRLEKGEQAMLFINRRGYATFVSCRSCGEAVKCPHCDVTLTYHKDGRMMCHYCGYTIR
                     QPKTCPSCGSPYIAPFGTGTQKIEEMATKMFPKARILRMDLDTTSKKGGHQQILSAFA
                     RGEADILIGTQMIVKGHDFPKVTLVGALAADLSLYASDYRCSEETFDLLTQAAGRAGR
                     GNAAGNVVIQTYQPDHYSIQTAAAQDYEAFYTQEMNFRKLLGYPPAVGLLTIQIADKN
                     EGRLEQAAALLEECLKQWIGQNEDLSKDGTIQFIGPVNAPVFKVNDIYRKILYIKQEN
                     YDILIHIR"
BASE COUNT          543 a          586 c          522 g          710 t
ORIGIN      
        1 ttcctgatgt gtatcagtat atcatagttt tcctgcttta tatataaaat ttttctataa
       61 atatcattaa ctttgaacac cggagcattt acagggccta taaactggat cgttccatcc
      121 tttgacagat cctcattttg cccgatccac tgcttcagac actcttccaa taacgctgcc
      181 gcctgttcta atcgtccctc atttttatct gcgatctgga tggttaaaag ccctactgct
      241 ggcggatatc caagaagttt cctgaaattc atctcctggg tataaaaggc ttcatagtcc
      301 tgggccgcag ctgtctggat gctgtaatga tccggctggt aggtctggat caccacattt
      361 ccagctgcat ttccacgtcc tgctcttcct gctgcctgag tcagaagatc aaaagtctcc
      421 tcactgcagc ggtaatcact ggcatagagg gaaagatccg ctgccagagc acctaccaaa
      481 gtgacttttg gaaaatcgtg gcctttgacg atcatctggg tgccaatgag gatatctgct
      541 tctcctcttg caaaagcaga taagatctgc tgatgaccgc cctttttaga agtagtatcc
      601 aggtccatgc gcaggatccg ggcttttgga aacatcttcg tggccatctc ttctattttc
      661 tgggttcccg taccaaaagg ggcaatatat ggggagccac aggaagggca tgttttcggc
      721 tggcggatag tataaccgca gtaatggcac atcatccggc cgtctttgtg ataggtcagc
      781 gtcacatcac agtgaggaca ttttaccgct tccccacagg atcggcagga cacaaaagtt
      841 gcatagcccc gtctgttgat aaataacatg gcctgctccc ctttttccag acggtcttct
      901 aacagttctt ttaatcttgc gctgaaaata gaccggttgc ccgcttttaa ttcttcccgc
      961 aggtcggcga tctccaccgt aggcagaaca gcatctgctt tggcacgtct ggtcagctta
     1021 taaaggtcat attcccctct tactgctctg gtataggttt ccaaagaagg cgttgcagag
     1081 ccaaggacca gcatggctcc ttccatttct ccccgttttc ttgccacttc cctggcgtca
     1141 tatctgggag aaagctcact tttataggaa ttttcatgtt cctcatcaat gatcaacatt
     1201 cccaatcgtt caaagggcac aaaaagagcg gatcttggac ctatgataat atcaatatcc
     1261 ccggttctgg cccgttcata ctggtcaaag cgctctcctg cggacatccg ggaatttagt
     1321 acagataccc ggtccccgaa acgaagataa aaacgcatga ctgtctgcca ggtaagggag
     1381 atctccggga tcagaaggat gacctgcttt ccagcccgga tcacatgact gatcagttcc
     1441 atgtacactt ctgtttttcc gctgcctgtg ataccgtgga taagacatgg ttttcggtcc
     1501 ccattgtcat atttttcaca gatattagct gcgatccttt gctgatcctt atttagaaca
     1561 ggcggtttcc actgggcttt agaagcgtct gttaaaaggc ggcgcatttc ctctaaggga
     1621 ttgcggtact ggttcaccac ctggactgca agaagtccct cctgttccag tggccggatg
     1681 gctgccgccg tcaggttcag cttatgaagg gtattttcat aaggaaggtc cggattttct
     1741 tttaaagcat ataatagtcg cagacgggcc ttgtattttt tccgctctgc tgtctggatc
     1801 gcagcttcca gagtcagcgc atccaccaga agatggatct tacgcttagg cgcttcccgc
     1861 accttttgct tcactggaag cactgttttt aatgcctggt tcatagtaga accataccgt
     1921 tccttcatcc accatgccag gtggatcagc tgggactggg cggtaatgct tccttctacc
     1981 agagagtcga ttttttttat tttcgatatg tcgtattccg cctggttggt caactccacc
     2041 acatagcctt tgcgtttatg gctgcctgcg ccaaaaggaa tatacacctg ctgtcccgca
     2101 gtgatctgcc ctaaaaggtg ctccgggatg cggtactgga aagtcttatc tacgctttcg
     2161 tgggatatgt cgatgatgat atttgcatat tgatagatgg ttggctgata tattgtatct
     2221 gttttattat ctgtattata attcaaattc tgtcacctga ttttaggctt tctgcatttt
     2281 ctggttgttt ccgtaccttt tattgtaaca gaaaatgaag ttcatgacaa cgcaaacagc
     2341 ctgaagttca tcgtttttca c
//
LOCUS       DBMDUA010000303         2359 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_15404_length_2359_cov_1.807352, whole genome shotgun sequence.
ACCESSION   DBMDUA010000303 DBMDUA010000000
VERSION     DBMDUA010000303.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15404_length_2359_cov_1.807352"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="ACZPCL_13665"
     CDS             complement(<1..257)
                     /locus_tag="ACZPCL_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HIR1163548.1"
                     /translation="MEAYALTDTGRVRSMNQDYIYASPEKVGSLPDLFLVADGMGGHK
                     AGDYASRFMVENLVVYLTHRHDGPCVTQIKEGIKTVNKALY"
     gene            complement(259..1323)
                     /gene="rlmN"
                     /locus_tag="ACZPCL_13670"
     CDS             complement(259..1323)
                     /gene="rlmN"
                     /locus_tag="ACZPCL_13670"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR1163549.1"
                     /translation="MLDLIDTKGKKDIKSMTLEEVTAEMTALGEKSFRAKQLYDWLHV
                     KLAESFDDMSTLSKDLRQKLKENYSLTALQVAGERISAIDGTRKYLFRLEDGNVIESV
                     WMQYHHGNSVCISSQVGCRMGCRFCASTLDGMERNLRPSEMLDQIYRIQTITGERVSN
                     IVVMGSGEPMDNYDNVVRFIRLISNEKGLNISQRNLTISTCGIVPGIKKFAEEGLQVT
                     LALSLHAPNDEVRKTLMPIANSFKLKDVLEACHYYFEKTGRRLTFEYSLVKGVNDNLE
                     EAKALTELIKDQHGHVNLIPVNPIKERDYVQSDRKAIEQFKNYLEKHGINATVRREMG
                     RDIGGACGQLRKSYLEEGEI"
     gene            complement(1339..2322)
                     /locus_tag="ACZPCL_13675"
     CDS             complement(1339..2322)
                     /locus_tag="ACZPCL_13675"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR1163550.1"
                     /translation="MSHSPHLNTSPYLRYSLPQWMYSMWEKDWGKENAEKIAESFLKE
                     RPVWVRCNFNLDSKEEILASLKAQQATAKPVEGFEAMLALSDYDYLESLEAFQKGWIQ
                     VQDISSALVGELAAPKKDSYVMDVCAAPGGKSLHIADKLQGTGCVEARDLTYQKVALI
                     EENASRMGAVNVKAVQWDATEYDPASENKADLVIADLPCSGLGIIGRKPDIKYHTTEE
                     NLKELAKLQREILSVVWKYVKPGGRLIYSTCTINKEENAENARWITENLPFKPEDLTE
                     AFPLKGEAFAGLKENTYPGADLKNGQIQILPGISPFEDFDGFFISVFKRTL"
BASE COUNT          576 a          589 c          472 g          722 t
ORIGIN      
        1 tcgtaaaggg ctttattgac tgtttttatc ccctctttta tctgggtaac acagggaccg
       61 tcatggcggt gggttaagta taccaccagg ttttccacca tgaaacggga agcataatcc
      121 cccgccttat gaccgcccat tccatctgct accaggaaaa gatccggcag ggagcctact
      181 ttttcagggg aagcgtagat ataatcctga ttcatggaac ggactcttcc tgtgtctgtc
      241 agtgcatatg cttccatatc agatctctcc ttcctccaga tagctctttc ttaactggcc
      301 gcaggcaccg ccgatatcac ggcccatttc cctgcgcacc gttgcgttaa tgccatgttt
      361 ttccagataa tttttaaact gctcaatagc cttccggtca gactgaacat aatcacgttc
      421 cttgatcgga ttgaccggga tcagatttac atgaccatgc tggtctttga tcagctctgt
      481 aagagccttg gcctcttcca ggttgtcatt tacccctttt acaagactgt attcaaaggt
      541 aagtcttctt cctgtttttt caaaataata atggcaggct tccagtacat cctttaattt
      601 aaaactgttt gcgatgggca ttaatgtctt tcgcacttcg tcatttgggg catgtaagga
      661 cagtgccagt gtgacctgaa ggccttcctc tgcaaatttt ttaattccag gaacaatgcc
      721 acaggttgag atcgttaagt ttctctggct gatatttaag cccttctcat ttgagatcag
      781 gcggataaag cggactacat tatcataatt atccatcggt tctccggaac ccatgactac
      841 gatattggac acccgctctc ctgtaatggt ctggatacgg tagatctggt ccagcatctc
      901 agaaggacgt aaatttcgct ccatgccatc cagagtagat gcacagaagc ggcagcccat
      961 acgacagccc acctgggaag aaatacatac agaattcccg tggtgatact gcatccacac
     1021 gctttcgatc acattgccat cttctaatcg gaacaggtat tttctggtac cgtcaatagc
     1081 tgaaatgcgc tctcctgcta cctgtaaagc ggtaagactg taattttcct tcagtttctg
     1141 acgcaggtcc ttggacagtg tgctcatatc atcaaagctt tctgccagct ttacatgaag
     1201 ccagtcataa agctgctttg ccctgaaaga tttctctccc agggctgtca tctctgctgt
     1261 cacttcttcc agtgtcatgg actttatatc ttttttacct tttgtatcga tcaggtctaa
     1321 catatatttc aatactcctt ataatgtacg cttgaaaaca ctgatgaaaa agccgtcaaa
     1381 gtcttcaaaa ggagaaatgc ctggaaggat ctggatctga ccatttttaa ggtctgctcc
     1441 cggatatgtg ttctctttca agcccgcaaa tgcttctcct tttaatggaa aggcttctgt
     1501 cagatcttcc ggcttaaaag gaagattttc tgtgatccaa cgggcatttt ctgcattttc
     1561 ctctttattg atggtacagg tgctgtagat cagacggccg cctggtttta cgtatttcca
     1621 tacaacagaa aggatctctc tttgcagctt tgccagttct tttaagtttt cttctgtggt
     1681 gtgatattta atatccggct ttcttccaat aattcccaga ccggagcagg gcagatccgc
     1741 aatcacaagg tccgctttat tttcacttgc aggatcatat tctgttgcat cccactgaac
     1801 agcctttaca ttgactgccc ccatacggct ggcattttct tcaataagag ccaccttctg
     1861 gtatgtaaga tcccttgctt ccacacagcc ggtcccctgt aatttatctg ctatatgaag
     1921 gctttttcca ccaggagctg cacatacatc cataacatag ctgtcttttt tcggcgcagc
     1981 cagttctcct actaaagcag agctgatatc ctgcacctgg atccagcctt tctgaaaagc
     2041 ttccaggctt tctaaatagt cataatcaga taatgcaagc atcgcctcaa aaccttctac
     2101 cggttttgct gtagcctgct gtgccttcag ggaagccagg atctcttctt tggaatccag
     2161 attaaaatta cagcgcaccc atacagggcg ttcctttaag aaagattctg caatcttttc
     2221 tgcattttcc tttccccagt ctttttccca catggaatac atccactgcg gaaggctgta
     2281 acgtaaatac ggactggtgt taaggtgcgg actgtgcgac actgatttta cggaacaata
     2341 ggaaacagtg caaaggtca
//
LOCUS       DBMDUA010000304         2331 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_15639_length_2331_cov_2.889686, whole genome shotgun sequence.
ACCESSION   DBMDUA010000304 DBMDUA010000000
VERSION     DBMDUA010000304.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15639_length_2331_cov_2.889686"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="ACZPCL_13680"
     CDS             <1..800
                     /locus_tag="ACZPCL_13680"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HIR1163551.1"
                     /translation="MKEYTITPINTGMVNSNKANYLYHNSTHKYYDVEGNIQLPVTVF
                     LVQSEDKKILIDTGMSSTEIANKYHHPGSVQPEGYAIYEQLEKMGISCDEITDIIYTH
                     LHWDHVYYTPMFKNAKLYAQRKEYEFALNPIPLYYKSYEEPHLGIHPQWEGREFVLLD
                     GECEVLDGISVYPTPGHSVGHQTVVVNTKEGQFHCCGDLIFTYDNLKPCPDIFYDITP
                     PSRFVNIVEEWHSIEELKKRAKSQEFILPTHAPEMIDLINSGRIIGK"
     gene            842..2068
                     /locus_tag="ACZPCL_13685"
     CDS             842..2068
                     /locus_tag="ACZPCL_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HIR1163552.1"
                     /translation="MAKTIALIASLDTKKKETLYAASVIQKQGFSTYIIDISTKNLVD
                     GADITPVEILKRYGIEWEDFDPLGKAASIDVMSKAAAFVLPQLYAEGKFDVVISIGGG
                     QNAKMAAMAMKTLPFAVPKIVASSLACGSRTMEQYVGDKDIFVMHTVADISGLNSITK
                     TVIQSVCSAAMGLAEYAKTFPSEEGKKVLGATELGVTSKGTENALHMIEESGLFENVV
                     FHANGVGGRCMENLMREGRIDVALDMTLHEITCETIGGYCVGANDRLMTAVEHKIPMV
                     VVPGALDMVDYFINEQGEGLPDDIDQRKKVYHNSSICHCKIYKEEAEKIVDVVCSRLN
                     QAVSPVTLVIPAKGCCEAAAPGGPMYDPEVDEVMVDAFKKKIGNNVKLVIVDANINEK
                     AFSETVAHEVLLLAHE"
     gene            2083..2259
                     /locus_tag="ACZPCL_13690"
     CDS             2083..2259
                     /locus_tag="ACZPCL_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163553.1"
                     /translation="MGTTIGIIGLFVAIALLVVLAFKGHSVIIAAPIAALIAVLFSYG
                     LKGHFRAYETFSVK"
BASE COUNT          741 a          435 c          549 g          606 t
ORIGIN      
        1 atatgaaaga atacacaatc acacctatta acactggaat ggtaaactct aacaaggcaa
       61 attatctgta tcacaacagc acccataagt attatgatgt agaaggaaat attcagcttc
      121 ctgtaactgt atttttagta cagagcgaag acaagaagat cctgatcgac accggaatgt
      181 ccagtactga gatcgccaac aaatatcatc atccaggttc cgttcagccg gaaggttatg
      241 cgatctatga gcagttagag aaaatgggca tcagctgcga tgagatcact gacattatct
      301 acacccattt acactgggat catgtttact atactccaat gtttaagaat gcaaaattat
      361 acgcacagag aaaggaatac gaatttgcat taaatccaat tcctctgtac tataagtcct
      421 acgaagagcc acatcttggc atccatccac agtgggaagg cagagaattt gttttattag
      481 acggagaatg tgaagttctt gacggaatta gtgtatatcc tacaccaggc cactctgttg
      541 gacaccagac cgtagttgta aatacaaaag aaggccagtt ccactgctgc ggcgatctga
      601 tctttacata tgataactta aaaccatgcc cggatatttt ctatgatatt acaccacctt
      661 ccagatttgt aaatatcgtt gaagaatggc acagcatcga agaattaaag aaacgtgcaa
      721 agagccagga attcatcctt cctactcatg cacctgagat gatcgatctt attaattctg
      781 gaaggatcat tggaaaataa cagtacattc cggcaaaagc cggaagaagg atggtagggg
      841 tatggcaaaa acaatcgcgt tgatcgcatc tttggataca aagaaaaaag aaaccctgta
      901 tgcagcatct gtgatccaaa aacagggatt ttccacctat atcattgata tcagtaccaa
      961 aaatctggta gacggggcag atatcacacc ggtggaaata ttaaaacgct acgggatcga
     1021 gtgggaagat tttgatccat taggaaaagc cgcaagtatt gatgttatga gcaaggctgc
     1081 agcctttgta cttccgcagt tgtatgcaga aggaaagttt gatgttgtca tttctattgg
     1141 aggcggacag aacgcaaaaa tggcggcaat ggcaatgaag acactgcctt ttgctgtgcc
     1201 gaagatcgta gcatcttctc tggcatgtgg aagcaggacc atggagcagt atgtaggaga
     1261 caaagatatt ttcgttatgc atacagtagc tgatatttcc ggtctgaaca gtattactaa
     1321 gactgtgatc cagagcgtat gcagcgcagc tatgggactg gctgagtacg ctaaaacctt
     1381 cccgagcgaa gagggcaaaa aagtccttgg agcgacagaa ttaggcgtaa cttctaaagg
     1441 aacagaaaat gctcttcata tgatcgaaga aagtggtctg tttgaaaatg tagtcttcca
     1501 cgcaaatggc gttggcggaa gatgtatgga aaatctgatg cgggaaggcc gtattgatgt
     1561 ggcgctggat atgacccttc atgagattac ctgtgagacc attggtggat attgcgtagg
     1621 tgcaaatgat cgtctgatga cagctgtaga gcacaaaata cctatggtag tggtgcctgg
     1681 tgctttggac atggtagatt attttatcaa tgaacaggga gaggggcttc cggatgatat
     1741 tgaccagagg aaaaaggtat atcataattc cagtatctgc cactgtaaga tctacaaaga
     1801 agaggcagag aagatcgttg atgttgtctg cagcaggctg aaccaggcgg tcagtccggt
     1861 aacactggta ataccggcaa aaggctgctg tgaagctgcc gctccaggcg gaccaatgta
     1921 tgatccagaa gtagatgagg tcatggttga cgcatttaag aaaaagatcg gaaataatgt
     1981 aaaactggtc attgtagatg caaacatcaa tgagaaggca ttttctgaaa ctgtagcaca
     2041 tgaggtactt ttacttgcac atgaataaag ggggatttat ttatgggaac taccattggg
     2101 atcataggat tatttgttgc catcgcactt ttagttgtac ttgcatttaa aggccacagc
     2161 gttatcattg cggcgcctat tgctgcactg atcgcggtac tgttttccta tggactgaag
     2221 ggacatttta gagcctatga aactttttct gtaaaataac ctaagtaagg tccttctatg
     2281 atacaataaa tatcattagg agggcctttt actatggcaa aacaaaagaa a
//
LOCUS       DBMDUA010000305         2321 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_15709_length_2321_cov_2.093694, whole genome shotgun sequence.
ACCESSION   DBMDUA010000305 DBMDUA010000000
VERSION     DBMDUA010000305.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15709_length_2321_cov_2.093694"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="ACZPCL_13695"
     CDS             complement(<1..332)
                     /locus_tag="ACZPCL_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1163554.1"
                     /translation="MADKKQKILIVDDSEMNRDILAVMLGSDYEILEAENGLEAIEIL
                     QQGMTDIDLILLDIMMPEMDGFDVLGFMKRSHMTEVIPVIMISSESSSSYIEKAYEMG
                     AADYISRP"
     gene            complement(501..>2008)
                     /locus_tag="ACZPCL_13700"
     CDS             complement(501..>2008)
                     /locus_tag="ACZPCL_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1163555.1"
                     /translation="RIQEIRHRIFREVARMAYHTEWPVDKRIEELPYKIIPGEVGNFR
                     NDVFLERAIVGERLRLAMGLPCRNASEHAPVSDNIEAADRAETYYTPPLINVIKFACN
                     ACKEKRVHVTDGCQGCLAHPCVEVCPKGAVTLDRTTGRSHIDEDKCIKCGKCATVCSY
                     NAIIVQQRPCAAACGMDAISSDENGKADIDYDKCVSCGQCLVNCPFGAIADKSQIFQT
                     IRAIQSGERVYAAVAPAFVGQFGPKVTPGKLRAAMKKLGFADVFEVAIGADLCAAQEA
                     QDFLDEVPEKIPFMATSCCPAWSVMAKKVFPKYANCISMALTPMTLTARLIKKQHESA
                     KVVFIGPCAAKKLEAMRTSIRSDVDFVLTFEEMAGIFDAKHVDIENIEEDPNGVNDAS
                     TDGRNFAVAGGVATSVKDVLKELKPDMEVKIANAEGLKECRQLLKLATLGKYDGYLLE
                     GMACPGGCVAGAGTMQPVKKSQAAVNIYASKANHKNCHETEYVKELDKLVD"
     assembly_gap    2009..2018
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          593 a          564 c          448 g          706 t
ORIGIN      
        1 aaaggacggc tgatatagtc agctgctccc atctcatatg ccttctctat ataggaagaa
       61 gaactttcag atgagatcat gatcaccggg atcacttctg tcatatggct gcgcttcata
      121 aagccaagca cgtcaaaccc atccatctcc ggcatcataa tatccaacag gatcagatca
      181 atgtctgtca ttccctgttg aaggatctct atagcttcaa gaccattttc agcttccagg
      241 atctcataat cactgcctaa cattacagcc agtatgtccc tgttcatctc tgaatcgtct
      301 acgatcagta ttttttgttt tttatctgcc atgtattttc aattacctct aaaacggaag
      361 ttcctggtat ataaaaacac tgctcccctt ctggtacacg aagcctgaaa catagacttt
      421 ctttcaccat caggaaagca gtgttttgag ttaccaaaat cacatattat tataaaggaa
      481 aatgtaattt aaaacaacca ctaatcaacc agtttatcca gctcttttac atattctgtc
      541 tcatgacagt ttttgtggtt tgctttggaa gcataaatat taacggctgc ctgggatttc
      601 ttaacaggct gcatggttcc agcgccggct acacaaccgc ctggacatgc cattccctct
      661 aacagataac cgtcatactt gcctaaggtc gcaagcttta acagctgacg gcactctttt
      721 aagccctctg catttgcgat tttaacttcc atatccggct tcagttcttt taatacatcc
      781 ttaacagaag ttgccacacc gcctgcaaca gcgaagtttc ttccgtctgt ggaagcatcg
      841 ttgacaccgt ttggatcttc ttcaatattt tcaatatcta catgctttgc atcgaaaatt
      901 ccggccattt cctcaaaggt cagtacgaaa tccacatcgc ttcggatgct ggtacgcatt
      961 gcttccagct tcttagctgc acacggtccg ataaatacaa cctttgcaga ctcatgctgc
     1021 ttcttgatca gtcttgcagt cagggtcata ggggtcagtg ccatggaaat gcagtttgcg
     1081 tatttcggga atactttctt tgccattaca gaccatgcag gacagcagga agttgccata
     1141 aatgggatct tctccggtac ttcatctaag aagtcctgtg cttcctgggc tgcacaaagg
     1201 tcagcaccga ttgctacctc aaatacatcg gcaaagccta atttcttcat agctgctctc
     1261 agttttccag gagttacctt tggtccgaac tggcctacga atgcaggagc tactgctgcg
     1321 tatacacgtt caccggactg gatggcacgg atggtctgga agatctggga cttatctgcg
     1381 atagcgccga atggacagtt tacaaggcac tgtccgcagg atacgcactt atcatagtca
     1441 atgtctgcct taccgttttc atcagaagag atagcatcca taccgcaggc tgctgcacat
     1501 ggtctctgct gaacgatgat agcattgtag gaacatacag ttgcgcactt tccacatttg
     1561 atacatttat cttcgtcaat atgtgagcgt cctgtagtac gatccagggt aacagctccc
     1621 tttggacaga cttctacgca aggatgagct aaacatccct gacatccgtc tgttacatgg
     1681 actctcttct ctttgcaggc gttgcaggca aatttgataa cattgatcag aggcggtgtg
     1741 tagtaggtct ctgctctgtc tgctgcttcg atattgtcag aaactggtgc atgctcagat
     1801 gcattacggc atggaagtcc cattgccaga cgcagtctct ctcctacgat cgctctctct
     1861 aagaatacgt cattacggaa gtttccaact tctcccggaa tgatcttata tggaagctct
     1921 tcaatacgct tgtctaccgg ccattcggta tgataagcca tacgggctac ttctctgaaa
     1981 attctgtggc ggatctcctg aatacgggnn nnnnnnnntc gctttctcct ctttgttgtg
     2041 atttttttaa cagttgcata tagatcaaaa aagttttatt aacataacag aaacagctca
     2101 ttcgggctgt ttctgttttt attatacgca tatacgaaat acattgcaag tgattttctt
     2161 tctcaatagc ttatgtttct gttaaaaaac aaacgaatta caggttattg accatttaaa
     2221 cgtccatatc tgcgggtcat accacggatc tcttctttta acaagtcagt cagctctcct
     2281 tttttcaggc gccatttcca gttttctcct acagtagaag g
//
LOCUS       DBMDUA010000306         2304 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_15867_length_2304_cov_1.976396, whole genome shotgun sequence.
ACCESSION   DBMDUA010000306 DBMDUA010000000
VERSION     DBMDUA010000306.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15867_length_2304_cov_1.976396"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="ACZPCL_13705"
     CDS             complement(<1..381)
                     /locus_tag="ACZPCL_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HIR1163556.1"
                     /translation="MKKYEKVFQKDYETWYGENEAYRKQVAEDPDRLAFHLMPETGWM
                     NDPNGLCQFKGEYHIYYQYTPFEPTGELKLWGHYKTKDLVTYEQCQPVLFPDSDEDAH
                     GVYSGSAFCENGKINFFYTGNVKLF"
     gene            complement(422..2302)
                     /locus_tag="ACZPCL_13710"
     CDS             complement(422..2302)
                     /locus_tag="ACZPCL_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HIR1163557.1"
                     /translation="MRNEEALSDLEGVKGVFLTQSQFQIIFGSGTVNLVCAEVQKQLG
                     TLEEQASDEKEEKSGNAIQRFIKMLSDIFVPIIPAIVAGGLLMGLNNVLTSPLINGSS
                     VIELYPQWAGLATAVNTFASAPFTFLPVLIGFSATKKFGGNAFLGAAMGMIMVHPDLL
                     NAYQIGIADPPVWNILGLNVAAIGYQGTVLPVLAVSWIIANIEKRLRKVTPSWLDNLT
                     TPLLSILITSFLTFIFVGPVLREAGNLLAAGITWLYNSLGFVGGAIFGLTYAPITMTG
                     MHHSFIAIETQLLADSVHTGGSFIFSTASMNNVAQGAAVLAVLLLTKNEKLKSICSAS
                     GVSALLGITEPAMFGVTLKLKYPFYAAIIGSGVGSAYLAWTKTLAQALGAAGLPGFIS
                     MKPEDYGHFAIGLILSMAVSFGLTVVFWKKMGIEKETAEVEAKTTDADKEAEVNSDVK
                     ATTDEIKPEAVVTEPEETAETAQENQELTFTSPMKGKVLPVTESADEMFASKMLGDGI
                     AVEAADGTVYAPCDGTVSLLFPTKHAVGIKSADGVELLIHVGINTVQLDGEGFEAFVT
                     QGDTVKKGDKLLKADLDFIREKGLNPQTMMIFPEAMNLDITPLSSENADENTAAVKVT
                     RK"
BASE COUNT          617 a          543 c          467 g          677 t
ORIGIN      
        1 aaacaatttt acatttcctg tataaaagaa gttgatcttc ccattttcac agaaagcaga
       61 gccggaatat acgccatgag catcctcatc gctgtccggg aataagaccg gctggcactg
      121 ttcataggta acaagatcct ttgttttata atgaccccac agttttaatt ctccggttgg
      181 ctcaaagggg gtatactgat aataaatgtg gtattctcct ttaaactgac agagaccatt
      241 gggatcattc atccagccgg tttccggcat caggtgaaat gcaagccggt ccgggtcttc
      301 tgccacctgt tttctgtatg cctcattttc cccataccat gtttcataat ctttctgaaa
      361 tactttctca tattttttca agggatgcct cctgctattg ccagctgtat tatttgtagt
      421 cttactttct ggttactttt actgccgcgg tattctcgtc tgcattttca ctggaaagtg
      481 gggtaatatc caggttcatt gcttccggga agatcatcat agtctgtgga tttaaacctt
      541 tttcccggat aaagtccagg tctgctttta aaagcttatc gcccttcttt actgtatcgc
      601 cctgggtaac aaacgcttca aacccttcgc cgtctaactg taccgtgttg atacctacgt
      661 gaatgagcag ttccacgcca tcggcagatt tgataccaac tgcatgtttg gtagggaata
      721 ataaagatac agtaccatca catggagcat aaacggttcc gtctgctgct tctactgcaa
      781 taccatcacc taacattttg gatgcgaaca tttcatctgc agactctgtt acaggaagga
      841 cttttccttt catgggggat gtaaaggtaa gttcctggtt ttcctgtgct gtttctgcag
      901 tctcttctgg ttctgttaca actgcttccg gtttgatctc atctgttgtt gctttcacat
      961 cagaattcac ttctgcttct ttatctgcat ctgtagtttt agcttctact tctgctgttt
     1021 ctttctcaat tcccattttt ttccagaata caacagtcag tccaaaggat actgccatag
     1081 aaaggatcag accgatggca aagtgaccat aatcttccgg cttcatggag ataaatccag
     1141 ggagtccggc tgcgcctaaa gcctgtgcca gtgttttagt ccatgcaaga taagcacttc
     1201 ctacaccgga accaataatt gctgcataaa atggatattt cagttttaag gtcacaccaa
     1261 acatcgccgg ctctgtgata cctaaaagag cggatacacc agatgcggaa caaatagatt
     1321 ttaatttttc gtttttggta agcagaagca ctgcaagaac agctgcgccc tgtgccacgt
     1381 tattcatact agctgtagag aagataaagc ttccgcctgt atgaacgctg tctgctaaaa
     1441 gctgagtctc aatggcaata aagctatgat gcataccagt catagtgatc ggtgcataag
     1501 taagaccgaa gatcgcgccg cctacaaaac caagagaatt ataaagccat gtaatacctg
     1561 ctgccagaag atttcctgcc tctcttaata caggtcctac aaaaatgaag gtaaggaagg
     1621 aagtaatcag aatagacagc agcggagttg tcaggttatc cagccaggaa ggtgttacct
     1681 tacgcaggcg tttttcaata tttgcaatga tccatgaaac agccagtacc ggaagaacgg
     1741 ttccctgata accaatagct gccacattca gccccaggat attccataca ggcggatctg
     1801 caataccgat ctggtatgca tttagaagat ccggatgtac catgatcata cccatagctg
     1861 cgcctaagaa ggcatttccg ccaaatttct tcgtagcact aaatccgatg agcaccggca
     1921 aaaatgtaaa tggcgcgctt gcaaaagtat ttactgctgt tgccaggcct gcccactgtg
     1981 gataaagctc aatgacactg cttccattta ttaaagggga tgtcagcacg ttgttaaggc
     2041 ccattaaaag accgcctgca acgattgccg ggataatagg aacgaaaatg tcactgagca
     2101 ttttgataaa gcgctggatg gcgttgccgc tcttctcttc tttttcatca gatgcctgct
     2161 cttctaatgt gcctaactgc ttctgcactt ctgcacacac cagatttaca gtgcctgaac
     2221 caaaaatgat ctgaaactgg gactgtgtaa gaaatactcc ctttacaccc tctaaatcac
     2281 ttaaagcttc ttcgtttctc agtg
//
LOCUS       DBMDUA010000307         2280 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_16098_length_2280_cov_2.846260, whole genome shotgun sequence.
ACCESSION   DBMDUA010000307 DBMDUA010000000
VERSION     DBMDUA010000307.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16098_length_2280_cov_2.846260"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="ACZPCL_13715"
     CDS             complement(<1..987)
                     /locus_tag="ACZPCL_13715"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR1163558.1"
                     /translation="MEKSEMRKLAEFIEKLSWDDLPEEVKTTVSFRVLDLISAALGAV
                     DDPLVKKVKASYLERNNGTGGKIWGSEGETDISTAAYINAMLAHTLELDDVHPASKTH
                     GSASLIPAAWSCARYVHASGKEFLTAVVCGYETVSRIGMALGVTSHRKKGWHATATCG
                     GFGCAAACGKLLGLNADELVSALGMAGTQSFGRWAFLGDGSTCKVLHPARAVVNGLDA
                     AFLAKAGMTGPEHILEAEDGGLLAAMSDTGDIAKVSKGLGTDWEILHMDMKPYPCCRS
                     AHCAIDCSLKLRDSILEKIGKEYDHKTLEEERKRLTEAIREIEIKTYEVGYKQ"
     gene            complement(1053..>2280)
                     /locus_tag="ACZPCL_13720"
     CDS             complement(1053..>2280)
                     /locus_tag="ACZPCL_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012550.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR1163559.1"
                     /translation="GKIKVDQGAIAGCAAGSFENICAVDTIAKKLNKGLGTFPLNIYP
                     ASQPIMYELNKAGVLNDLMTYGVRVKTAFCGPCFGASDAPGNNDFCIRHSTRNFPNRE
                     GSNPATGQIASVALMDSKSIAATAFNGGYLTSAEDVPVEYYTPEYHFNSDIYKNIVYN
                     GYKNPKPETEIVYGPSIKDWPKMVALTDNLLLQVASVIRDEVTTTDELIPSGETASYR
                     SNPYKISEFTLIRKDPHYVGNAKAIQKYETARRNGDAKKTAEVYEVLKAAGVTADTEE
                     LMKTTGIGSVLYAKRPGDGSAREYAASCQKVLGGLANICIEYATKRYRSNCVNWGILP
                     FTNPDKEIVLEPGEYVYLPGIRKAVEEGATKVTATVIGTDGSTRPMELELKNVTEAEK
                     KVLLAGSLINFYKEEK"
BASE COUNT          533 a          585 c          451 g          711 t
ORIGIN      
        1 ttgcttatat cccacttcat aggtcttgat ctcaatctcc ctgatagctt ctgtcagcct
       61 ttttctctct tcttctaagg tcttatgatc gtactctttt ccaatttttt ccaaaatact
      121 gtctcttaat ttcagactgc aatctatagc acaatgggca ctgcggcagc agggatatgg
      181 cttcatatcc atatgaagga tctcccaatc tgtaccaaga cctttactga cttttgctat
      241 atcaccagta tcagacatag ccgccagtaa gccgccatcc tctgcctcca aaatatgctc
      301 cggtccggtc attccggctt ttgctaaaaa tgctgcatcc agaccattta caaccgctct
      361 ggccgggtgg agcactttgc aggtgcttcc atcccctaaa aaggcccatc tgccaaaact
      421 ctgggttcct gccatcccaa gagccgatac cagctcatct gcattcagcc ccagaagctt
      481 tccgcaggca gctgcgcagc caaaacctcc gcaggtagca gtggcatgcc agcccttttt
      541 tctgtgactt gttactccca acgccatacc gatccggctg acggtttcat agccacatac
      601 cactgctgtg agaaattctt ttccagaagc atggacatac cgggcacagc tccatgcagc
      661 cgggatcagg ctggcgcttc catgtgtttt tgacgccgga tggacatcat ccagttccag
      721 agtatgggcc agcatggcat ttatataagc agctgtagaa atatccgttt ctccttcgct
      781 tccccagatc ttaccgcctg ttccgttatt tctctctaaa taagaagctt ttactttttt
      841 aaccagcgga tcatccacag ctcccaaagc agcgctgatc agatccagga cccggaaaga
      901 tactgtggtt tttacttctt ccggcaggtc atcccagcta agtttttcta taaactctgc
      961 cagtttacgc atctcacttt tctccataaa tgctccttct attcccttac tctttggatc
     1021 tttttgggat atcatcttgc ttttacttct tattattttt cttccttata aaagttgatc
     1081 agagaacctg caagaagtac cttcttttct gcttctgtta catttttaag ttccagttcc
     1141 attggtctgg tgcttccatc tgttccgatc accgtagctg ttacctttgt ggctccttct
     1201 tctactgctt tgcggatgcc tggaagatat acatattctc ctggttccag aacaatttcc
     1261 ttatccggat tggtaaatgg cagaatgccc cagtttacac agttgctgcg gtaacgcttg
     1321 gttgcatact caatacagat attggcaagt cctccaagaa ccttctggca gctggctgca
     1381 tattctcttg cagaaccgtc acctgggcgt tttgcataaa ggacagagcc aattcctgta
     1441 gtcttcatca gctcctctgt atctgctgtt actcctgctg cttttaatac ttcataaact
     1501 tctgctgtct tttttgcatc cccgtttctt ctggctgttt catatttctg aatggctttt
     1561 gcatttccca cataatgagg atccttgcgg atcagtgtaa attcagagat cttatatgga
     1621 ttagagcgat aggatgccgt ttcaccagaa gggatcagtt catcagtagt agttacttca
     1681 tcacggatca cactggctac ctgaagcaat aagttgtctg taagggcaac catctttggc
     1741 caatctttaa tggaaggacc ataaacgatc tctgtctcgg gctttggatt tttatagccg
     1801 ttgtatacaa tattcttata aatatcactg ttaaaatgat actctggagt gtaatattct
     1861 accggcacat cttctgcgga agtcagataa ccgccgttaa atgcggttgc tgcaatggac
     1921 ttggagtcca taagcgcaac ggaagcgatc tggccagttg ccggattgga gccttcacgg
     1981 ttggggaagt ttctggtact gtggcggatg cagaaatcat tatttcccgg agcatctgaa
     2041 gcgccaaagc aagggccgca gaaagcagtt ttcactctta caccgtaagt catcaggtca
     2101 tttaatacac ctgctttatt taactcatac atgatcggct gggaagccgg ataaatatta
     2161 agtgggaatg ttcccaagcc tttgtttaat ttctttgcaa tggtatctac tgcacagata
     2221 ttttcaaagg atcctgctgc acagccggca atagcgccct ggtcaacctt gatctttcca
//
LOCUS       DBMDUA010000308         2254 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_16353_length_2254_cov_3.619601, whole genome shotgun sequence.
ACCESSION   DBMDUA010000308 DBMDUA010000000
VERSION     DBMDUA010000308.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16353_length_2254_cov_3.619601"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(63..530)
                     /locus_tag="ACZPCL_13725"
     CDS             complement(63..530)
                     /locus_tag="ACZPCL_13725"
                     /inference="COORDINATES: protein motif:HMM:NF014052.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HIR1163560.1"
                     /translation="MFRFRFKFLADSYLLLFSFFLGMIGAALFFWWRYPVFPSYLFYS
                     YGITGNKNFTEAFLIVIKQRFFQLGAGWLCGLTVYSVWLFGILTCFWSFSLGMGVIYF
                     TLEKGLWGLPLFLRSLLPQGIFYLMVWYVLSRWSFSTRKRLRLLYRIKIVRRC"
     gene            729..1556
                     /locus_tag="ACZPCL_13730"
     CDS             729..1556
                     /locus_tag="ACZPCL_13730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1163561.1"
                     /translation="MSQYQLIAFDMDGTLLNSNKQISPETLNAIKRATDAGKTVILCT
                     GRNLAELNAFTEIIPGLRYLDCVSGACVYDLKEKKTLYFQALDPGIVKKLMEFGMEEG
                     AMIHVLSERSIVQKDQQSHMADFHMGIYQTMFDQITDKLENIYNSFCEEPFPVLKLNL
                     YHRDEASRARTVERIKAAGLETALALAESTSLECSAKGIDKGVGLKKLCEHLGLPLEQ
                     TIAVGDADNDTMALKTAGLAVAMGNATEPIKALADVIVADCDHNGCAEAIDKYLLAD"
     gene            1629..2114
                     /locus_tag="ACZPCL_13735"
     CDS             1629..2114
                     /locus_tag="ACZPCL_13735"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1163562.1"
                     /translation="MKIRTATLNDLDALTAVEAECFPAAEAATKKDFENRLAYYADHF
                     WLMFDENDKLIAFVDGMVTDEADLTDEMYEKGEMHNENGAWQMIFGVNTIPSCRKKGY
                     AGELIKAAIEDARKQGRKGLVLTCKDKLIHYYAKFGFVNEGVSPSVHGNVVWYQMRLT
                     F"
BASE COUNT          707 a          497 c          490 g          560 t
ORIGIN      
        1 caaaaggggc cacatcatct ccacttcgat gatagtaatc atataatgct ttcagtatca
       61 tctcaacacc tcctcactat ctttatccgg tacaacaatc gcagcctttt tcttgtacta
      121 aaagaccatc tggaaagaac gtaccagacc atgagataaa atatcccctg aggaagaagg
      181 gaacgcaaaa acagaggcag tccccagagt cccttttcca atgtaaaata aataactccc
      241 attcccaggc tgaaagacca gaaacaggtc agtatcccaa acagccatac actgtaaacc
      301 gtaagcccgc agagccaccc tgcacccagc tggaaaaaac gctgttttat gacgatcaga
      361 aacgcttctg taaagttttt gtttcctgtt attccataac tgtaaaataa ataagaagga
      421 aatacaggat aacgccacca gaaaaataac gctgccccta tcattcccag aaaaaaacta
      481 aacaacagca gataactgtc tgccaagaat ttgaagcgaa atcggaacat ggactgcctt
      541 tcctgctttt tcaagcgcct ttcctgtgct cttataccag ttgtatgccc tttgcagcct
      601 gtgcatgatt atttttgtac acggttaggg aaatatgata taatcattaa cgaacgaacc
      661 gcctgcaata gcaggcagat aagcacgcca gtgcggggtt cgtgagctta cccttggagg
      721 attttaccat gagtcaatat cagttgatcg cctttgatat ggatggaact cttttaaatt
      781 caaataaaca gatctcccct gaaactttaa acgccattaa acgggcaacc gatgcaggaa
      841 aaactgttat cctgtgtacc ggccgcaacc tggctgaact gaatgcgttt acagagatca
      901 taccgggact gcgctattta gactgtgtca gcggtgcctg tgtttatgac ctaaaagaaa
      961 agaaaacact gtatttccag gctcttgacc ctgggatcgt aaaaaagctg atggaatttg
     1021 gcatggaaga aggagccatg atccatgtac tttcagagcg ttccattgtc cagaaggatc
     1081 agcagtccca tatggctgat tttcatatgg gcatttacca gaccatgttt gatcagatca
     1141 ctgacaaact ggaaaatatt tacaattctt tctgtgaaga gccatttcct gtgctgaagc
     1201 tgaaccttta ccacagagac gaagcctcac gggcgcgcac cgtggaacgc attaaagcag
     1261 ccggtcttga aactgcactg gctctggcag agtctacctc tttagagtgt tctgccaaag
     1321 ggatcgacaa aggtgtagga cttaaaaagc tgtgtgaaca tctgggactg ccattagaac
     1381 agactattgc tgtaggtgat gcagataacg ataccatggc attaaaaact gccggtctgg
     1441 ccgttgccat gggaaatgca accgaaccca tcaaagccct ggctgatgtg atcgtagctg
     1501 actgcgacca taacggatgc gcagaagcca ttgataagta tctgctggca gattaaacat
     1561 aacagcctgc gcctctggcg cattgtttgc atgaacattt atacaatatg gaggatttaa
     1621 atactattat gaaaatcaga actgcaacat taaatgactt agacgcatta actgctgtag
     1681 aagcagagtg tttcccggct gctgaagcag ccacaaaaaa ggactttgaa aaccgcctgg
     1741 cttattatgc agaccatttc tggctgatgt ttgatgaaaa tgacaaactg atcgcctttg
     1801 tagacggtat ggtcacagat gaagccgacc tgacagatga aatgtatgaa aaaggagaaa
     1861 tgcataatga aaatggtgca tggcagatga tcttcggggt caataccatt ccttcctgcc
     1921 gcaaaaaagg ctatgcaggt gaactgatca aagctgctat tgaagacgcc agaaaacagg
     1981 ggcgcaaagg cctggtactt acctgcaagg acaaactgat ccattactat gcaaaattcg
     2041 gtttcgtaaa cgaaggcgtt tccccgtctg tccacggaaa tgtggtatgg tatcagatga
     2101 gactgacttt ttaaataaat gatatgatac cagggtcaaa aggtctaaaa tccgacacaa
     2161 gcttgcttgc aggaggattt ttgaacttgg gacccgcaaa aaacatgagt aagtagccat
     2221 ttttgcaaaa aatgagcgga ttactaatgg tttt
//
LOCUS       DBMDUA010000309         2227 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_16621_length_2227_cov_3.170273, whole genome shotgun sequence.
ACCESSION   DBMDUA010000309 DBMDUA010000000
VERSION     DBMDUA010000309.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16621_length_2227_cov_3.170273"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(60..986)
                     /locus_tag="ACZPCL_13740"
     CDS             complement(60..986)
                     /locus_tag="ACZPCL_13740"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1163563.1"
                     /translation="MFTPADTISIPTSRLKENIPDIAAITGNTLWGFSLLLSKTALNY
                     APTSILLSMRFIIAFLLMNLILLFKIERISLKGKNILPVLLLALNDVAYFYCESYGIA
                     LTNSMFTGVVLSLIPILAMGLSALFLKEFPTLRQILFSFLPVAGVVIIILAGSSSGAI
                     QPVGILFVLSACFLSATQRVINRKAAVNFSAFERTYFTICSSCLFFTTNAVHQLGGNA
                     MKLLHYATIPGFIIPVLILSIFCSVTCNILVNYAAGKMSVGRLAAFSNLQTMWSMFAG
                     VLFLREPFGLSSFIGSVLIIYGIWHVTKRNIP"
     gene            1262..2173
                     /locus_tag="ACZPCL_13745"
     CDS             1262..2173
                     /locus_tag="ACZPCL_13745"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1163564.1"
                     /translation="MDQEILAFLEICRCKSISKAAEKLFISQSSLSTKIRILERKLGY
                     SLFIRGKGSRSVMLTDKGEKFLGLAVKYQEILEKMLALGRDGGSEKLRISSMNSLGTH
                     LLTPVYERFMAENPDIILEIKDADQAHLGVEEGITDLAFTVVKVESATVQVRPVFAEP
                     MVFLCAEDSRYMDSWNERNAEKVEKDQLDLNHEVYVDWFYEFVHWHSQLFQGRQPKLA
                     LSIMNQLQFFLRKRDSWAFVPASVAVGLLAEGGIKKLPVDFKVPPRVVNYMCMEKAAG
                     SRKIQLFLQCLRQVLLEMEQVEFEIYI"
BASE COUNT          689 a          350 c          605 g          583 t
ORIGIN      
        1 tgtaaatttt ttaaattgag tataacacag gaaagccggg gcgtacagat aggaaaaaat
       61 taaggtatgt ttctttttgt aacatgccat atgccgtata tgatcagaac cgaaccaata
      121 aaggaggaca gaccaaaggg ctcccgaaga aataatactc ctgcaaacat ggaccacatg
      181 gtctgcaggt tggagaaagc agcaagcctg ccgacggaca tttttccagc tgcataattt
      241 accaggatat tgcaggtaac agagcagaag atactaagga tcagtaccgg gatgataaag
      301 ccggggatgg tggcgtagtg aagcagtttc atggcattac cgcccagttg gtggactgca
      361 ttggtggtga aaaagagaca ggagctgcaa atagtgaaat aagtgcgttc aaatgcagaa
      421 aaatttacag ctgctttcct gttgatcaca cgctgggttg cagataaaaa gcaggcagaa
      481 agaacaaaaa ggatgcccac cggctggatt gcgcctgagg agctgccggc caatatgatg
      541 atgactacac cggctaccgg gagaaagctg aaaagaatct ggcgcagagt gggaaattct
      601 tttaagaaga gagcagaaag tcccattgcc aggatcggga tcagggataa tactacacct
      661 gtgaacatgc tgttggtaag ggcgatgcca tagctttcgc agtaaaaata ggctacatca
      721 ttcaatgcca gaaggagtac aggaaggatg tttttgcctt ttagcgagat gcgttctatt
      781 ttaaaaagaa ggattaaatt cattagtaaa aatgcgatga taaagcgcat agacagcaga
      841 atactggtgg gggcatagtt taaagctgtc ttggacagaa gaagactgaa gccccagaga
      901 gtgttgccgg taatggcagc aatatcaggg atgttttctt ttagacggga tgttgggatg
      961 gaaatagtat cagcgggtgt aaacatggaa acctccaaaa gtttttgatg tcctatcagg
     1021 atcttacatt tggttaacgt gtttttaata caagctgagt gtatcattgc aggtatatct
     1081 tgtaaaatac agaaaaatca tatattatat tgaatggatt gaatatattt gactggtaca
     1141 gttattttga aaacgatgta ctagtacacc gtttcgaaaa tgatgtactg gtattggatg
     1201 aaacatcagg tctgttttat tgatagaaaa tcgttaaaag agaaatgggg aaagaaagga
     1261 tatggatcag gagatactgg catttttaga gatctgccgg tgtaagagca tttcaaaagc
     1321 agcggaaaaa ttattcatca gtcagtcatc cttaagcacc aagatccgta ttttggagcg
     1381 gaaattaggt tattctcttt ttataagggg aaagggaagc cgcagtgtaa tgctcacaga
     1441 taagggagaa aaatttctgg gactggcagt gaaatatcag gagatactgg aaaaaatgtt
     1501 ggctttaggc cgggatggag gaagcgagaa gcttcggata tcttcgatga acagtttagg
     1561 aactcatctg ctgacacctg tgtatgagcg gtttatggcg gaaaatccgg atattatact
     1621 ggagataaag gatgcggatc aggctcatct gggggtggaa gaagggataa cagaccttgc
     1681 ttttacagtg gtaaaagttg agtctgctac tgtgcaggtg cgtcctgttt ttgcggagcc
     1741 tatggtgttt ttgtgtgcag aggacagccg gtatatggac agctggaatg agagaaacgc
     1801 agagaaagtg gaaaaggatc agttggatct gaaccatgaa gtatatgtgg actggtttta
     1861 tgaatttgtc cactggcaca gtcagctgtt tcaggggcgg cagccgaagc tggcattatc
     1921 tattatgaac cagcttcagt tttttctgcg taaaagagat agctgggctt ttgttccggc
     1981 cagtgtggct gtggggcttc tggcagaagg cgggataaag aagttgccgg tggattttaa
     2041 agtgccgcca agggtggtca attatatgtg catggaaaag gcagcgggaa gcagaaaaat
     2101 ccagcttttt ttacagtgcc tgcgccaggt gcttttagaa atggaacagg tggagtttga
     2161 gatttacata tagaagaata tattaaagat attcgtgatc cgcttatttt tctttgaaaa
     2221 ttggtgt
//
LOCUS       DBMDUA010000310         2223 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_16666_length_2223_cov_2.621112, whole genome shotgun sequence.
ACCESSION   DBMDUA010000310 DBMDUA010000000
VERSION     DBMDUA010000310.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16666_length_2223_cov_2.621112"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            33..1241
                     /locus_tag="ACZPCL_13750"
     CDS             33..1241
                     /locus_tag="ACZPCL_13750"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1163565.1"
                     /translation="MEKEFDILTGAKRLDEVRFSKIRTVMDMVADLRAKGEKVLALSA
                     GEPDFDTPAPIKEAVIKALEENYTHYGSNRGYPALRKLLSEKLEKETGVSYDPETEII
                     FTTGGAEALNNAFMAVVDPGDEVIVFSPAFITYENLVHMCGAKLVKVPLKKENNYQID
                     MDDLKAAVTDKTKLLVMNNPNNPTGAVYSKECLEQVCELAKEKNFLILSDEMYSRLVY
                     GDGKFYSIASFPDMKERTIIINGFSKTYAMTGWRVGYVAAPAKLLPSILKVHQYCSTC
                     CPTFIHVGLANALDLPETEEAVCKMVDAFAKRREVLIEGLDQIPGITYVKPEGAFYAM
                     VDVSSFGVDGDTFAAKLLKEKHVAVIPGSGLGENCKDAVRISFAASEDTIRKATARMK
                     EFIQEKAYES"
     gene            1231..2139
                     /locus_tag="ACZPCL_13755"
     CDS             1231..2139
                     /locus_tag="ACZPCL_13755"
                     /inference="COORDINATES: protein motif:HMM:NF015509.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR1163566.1"
                     /translation="MNLSAVINLQLVMFALFICGFILTRKGVINSERRKGMSDLLIDL
                     FLPANIICAFLTGISRDVFAEAFQVLVIALGIQIISYLLGLVLYRKEVNGRRMVLRYA
                     TICSNAGFMGNPVVEGIYGSQGLLYASVYLIPLRIFMWSAGLSCFTVASPKDIVKKLV
                     HHPCIIAVVIGMALMPFPDVIPSFLLQTMQSCSKCVLPVSMIVIGSILSEADIRKMLK
                     GSTLFYCVVRLLGIPAVTMVVCRLLGMGALVTGVCTILAGMPAGSTTAILAEKYDGDA
                     KYASECVFLSTVLSLFTMPLLYMILA"
BASE COUNT          611 a          446 c          562 g          604 t
ORIGIN      
        1 ggtacaaaat cagggaaaag agggacctag atatggaaaa agagtttgat atattaacag
       61 gtgcaaaacg actggatgaa gtacggtttt ccaagattcg tacagtaatg gatatggtag
      121 ctgaccttcg tgccaaaggg gagaaagtgc tggcattaag cgccggagaa ccggattttg
      181 acacaccggc ccctattaaa gaggcagtga tcaaagcctt ggaagaaaat tatacccatt
      241 atggctccaa cagaggctat ccggctctta gaaaacttct gtcagagaaa ctggaaaaag
      301 agacaggggt ttcctatgat ccggagacag agatcatttt caccaccggc ggtgcagaag
      361 ccttaaacaa tgcctttatg gcagtggtag atccgggaga tgaggtgatc gtattttcac
      421 cggcttttat cacctatgaa aatctggtcc atatgtgcgg tgcaaaactg gtaaaagtgc
      481 ctttaaagaa ggaaaataac tatcagatcg atatggatga tctgaaggct gctgttacag
      541 acaagacaaa gcttctggtg atgaacaatc caaacaaccc aacgggcgct gtttacagca
      601 aagagtgctt agagcaggta tgtgagctgg caaaggagaa gaacttcctg atcctttctg
      661 atgaaatgta ttccagactg gtgtatggag acggaaaatt ctattctatt gcctctttcc
      721 cggatatgaa ggagaggacc attatcatca atggtttttc caagacctat gcaatgaccg
      781 gctggagagt aggctacgtg gcagctcctg caaagcttct gccaagtatc ttaaaggtac
      841 atcagtactg cagcacctgt tgtcctacct ttatccatgt gggtcttgca aatgcactgg
      901 acctgccgga aacagaagag gcagtgtgca aaatggtaga tgcgtttgca aagagacggg
      961 aagtgctgat tgaaggactg gatcagatcc cgggaattac ctatgtgaag ccggaaggcg
     1021 ctttttatgc catggtggat gtatcctctt ttggggtaga cggagatacc tttgcagcaa
     1081 agcttttaaa ggaaaaacat gtggcagtga tcccgggctc cggtcttggg gagaactgca
     1141 aggacgcagt gcgtatttcc tttgcagcat cagaagatac cataagaaag gcaacagcgc
     1201 ggatgaaaga gtttatccag gagaaagctt atgaatctta gtgcagtgat caaccttcag
     1261 ctggtgatgt ttgccctgtt tatctgtggc tttatcctta caagaaaagg agtcatcaac
     1321 agtgagcggc gaaaaggcat gtctgacctg ctcatcgacc tgttccttcc ggcaaatatt
     1381 atctgcgcct ttctgacggg catcagcagg gacgtatttg cagaggcctt tcaggtgctt
     1441 gtgatcgctc ttgggatcca gatcatttcc tatctgttgg gactggtatt atataggaag
     1501 gaagtaaacg ggcggagaat ggtcctgcgc tatgcaacta tctgttccaa tgccggattt
     1561 atgggaaatc cggtagtgga agggatctat ggttcccagg gcttactgta tgcttctgtt
     1621 tatctgatcc ccttaagaat ctttatgtgg tctgccggtc tttcctgctt tacagtggca
     1681 agtccaaagg atattgtaaa aaaactggtc catcatccct gtatcattgc agtagttatc
     1741 ggtatggccc tgatgccatt tcctgatgtt attccaagtt tcctgcttca gaccatgcag
     1801 tcctgcagca aatgtgttct tcctgtttcc atgatcgtca ttggaagtat tttgtcggaa
     1861 gcagatatcc gtaaaatgtt aaagggaagc accttatttt actgtgtcgt gcgccttctg
     1921 ggaattccgg ctgttaccat ggtcgtatgc cgtcttcttg gaatgggggc gctggtaaca
     1981 ggtgtatgta ccatcttagc aggaatgcct gcaggaagta ccacagcgat ccttgcggaa
     2041 aaatatgacg gggatgcaaa atatgcttct gaatgtgtct ttctgtctac ggtactttcc
     2101 ctttttacca tgcctctttt gtatatgata ctggcctgaa tatttttgct gaactgtaac
     2161 gaaatagtgc acaatagcag ccggattgta attagttaaa actaacaaaa ataaagaaaa
     2221 ctg
//
LOCUS       DBMDUA010000311         2215 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_16741_length_2215_cov_2.305109, whole genome shotgun sequence.
ACCESSION   DBMDUA010000311 DBMDUA010000000
VERSION     DBMDUA010000311.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16741_length_2215_cov_2.305109"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /gene="mobB"
                     /locus_tag="ACZPCL_13760"
     CDS             complement(<1..1068)
                     /gene="mobB"
                     /locus_tag="ACZPCL_13760"
                     /inference="COORDINATES: protein motif:HMM:NF024214.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HIR1163567.1"
                     /translation="MKMEQYGCVLLKGGKSNRMGGSDKAKLLYRGKSFEERIKGELEQ
                     LGLPVFVSEAKKSPDPRAIPDLIPEAGPLGGIYSCLKQTGLDGLFFVSCDMPLFASYL
                     GKRTLMEAGDKWDAVIWKTRDGRIHPVCGWYSARCLPVLKKQLLEKNGKVRDLLGKLQ
                     VKVLETENEHVPDRWFLNVNTPQALGELTCLKAPVFAVSGKKNTGKTTLTETLVREFS
                     VRGLKVAVIKHDGHDFVPDVPGTDSYRHKAAGAVGTIVYSENRFCLVKEEKGHEAEDF
                     LPFFQDMDLILVEGLKDSHYAKIEVMRRDVSKKAVCRKETVKAYVTDFRPDTWSGFDA
                     ESMSDCPVYDFHEIDAIMEIVL"
     gene            complement(1200..>2215)
                     /locus_tag="ACZPCL_13765"
     CDS             complement(1200..>2215)
                     /locus_tag="ACZPCL_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HIR1163568.1"
                     /translation="LAKVLDGRPLDYIVVDHMEPDHAATLGQIAEKHPEAKIVCNAKT
                     VGIIKQFFDLDIDSRSVVVKEGDTLCTGKHNLTFVMAPMVHWPEVMVTYDTTDKTLFS
                     ADAFGTFGAMNGNLFADEVNFERDWLDDARRYYTNIVGKYGMQVQTLLKKAAGLEIAT
                     ICPLHGPVWRENISWYVDKYLTWSSYAPEEKSVMIAYGSIYGNTENAANILACKLADK
                     GVRNIAVYDVSATHPSQIVSEAFRCSHLVFAAATYNGGIFTNMEHVLNDLKAHGLQNR
                     TVAVIENGTWGVVAGKQMKEILSGMKNMNILEQTVTVKSALKDEQDAQLEALADAIVA
                     SLQ"
BASE COUNT          566 a          570 c          410 g          669 t
ORIGIN      
        1 taaaacgatt tccataatag cgtcgatctc atgaaaatca tacaccggac agtctgacat
       61 agactctgca tcaaatccgg accatgtatc cggcctgaaa tctgtaacat acgcctttac
      121 agtctctttt ctgcacacag ccttttttga aacatcacgg cgcatcactt ctattttggc
      181 ataatgggag tctttcagtc cttccaccag gatcagatcc atatcctgaa aaaaaggcaa
      241 aaaatcttct gcctcatggc ccttttcctc ttttaccaga cagaaacgat tttcagaata
      301 gactatcgtt cccactgccc ccgccgcttt gtgacggtaa ctgtctgttc ccggtacatc
      361 cggcacaaaa tcatgaccat cgtgtttgat cacagccacc ttaagcccgc gcacggaaaa
      421 ttcccggact aaagtttccg taagagtcgt cttgcccgta tttttctttc cgctgactgc
      481 aaataccggt gctttcaagc atgtaagttc tcctaatgcc tggggagtat tcacatttaa
      541 aaaccaccgg tcaggtacat gttcgttttc tgtttctaaa acctttacct gcagttttcc
      601 taacagatca cgtactttcc catttttttc caaaagttgt tttttaagaa ccggcagaca
      661 tctggctgaa taccagccgc acaccggatg gatccgcccg tctcttgttt tccagatcac
      721 agcatcccat ttatctcctg cttccataag agtgcgtttc cccagataag atgcaaataa
      781 gggcatatcg caggaaacaa aaaacagtcc atcaagaccg gtctgtttta aacaggaata
      841 gatccctcct aaaggtccgg cctctgggat cagatcaggg attgccctgg gatccgggct
      901 tttttttgcc tctgatacaa aaaccggcaa tcccagctgc tccagttctc ctttgatccg
      961 ttcttcaaaa ctttttcccc tgtaaagaag cttggcttta tcacttccgc ccatgcggtt
     1021 ggactttccg cctttaagca ggacacatcc atactgttcc attttcattt tccttctctt
     1081 tatcatgaaa tacgtacacc tggaaatctt tcgttatttt ccaatatcca ggtgtaaaaa
     1141 agcaggaagc catatatccc gcttcctgcc tttttatttt ctaatgtact tacaagatct
     1201 tactgtaaag atgcaacaat agcatctgcc agtgcctcta actgtgcatc ctgctcatcc
     1261 tttaaagcag atttaacagt gacagtctgc tctaaaatgt tcatgttctt catgccggaa
     1321 aggatctctt tcatctgctt tcctgcaaca acaccccagg taccattttc gatcacagct
     1381 acagtacggt tctgcagtcc atgtgccttt aagtcattta aaacatgttc catattggta
     1441 aagatgccgc cattataggt agctgctgca aataccagat ggctgcagcg gaatgcttcg
     1501 cttacgatct gggatggatg ggttgcagat acatcataaa ctgcaatatt tctcacaccc
     1561 ttgtcagcca gcttgcatgc caggatattg gctgcgtttt ccgtatttcc gtaaatagaa
     1621 ccataggcga tcataacact cttttcttcc ggtgcatagc tgctccaggt taagtattta
     1681 tctacatacc agctgatatt ttcacgccat acaggtccat gtagcgggca gatggtagcg
     1741 atctccagtc ctgctgcttt cttaagcagt gtctgtacct gcatgccata ctttccaacg
     1801 atattggtat aatatcttct tgcatcatcc aaccagtcac gctcaaagtt tacttcatct
     1861 gcaaacaggt tgccattcat ggcgccgaaa gtaccaaaag catctgcgga gaataaagtc
     1921 ttgtctgtgg tatcgtaagt aaccataact tccggccagt gtaccattgg tgccattaca
     1981 aatgtaaggt tatgcttacc ggtgcagaga gtatctcctt cttttactac tacggatctg
     2041 gagtcaatat caagatcaaa gaactgcttg ataataccaa ctgtctttgc attgcatacg
     2101 atctttgctt ccggatgctt ttctgcgatc tgtcctaagg ttgcagcatg gtctggttcc
     2161 atgtggtcta ctacgatata atctaatgga cggccgtcta aaacctttgc caggt
//
LOCUS       DBMDUA010000312         2211 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_16785_length_2211_cov_2.031754, whole genome shotgun sequence.
ACCESSION   DBMDUA010000312 DBMDUA010000000
VERSION     DBMDUA010000312.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16785_length_2211_cov_2.031754"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="ACZPCL_13770"
     CDS             complement(<1..1256)
                     /locus_tag="ACZPCL_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR1163569.1"
                     /translation="MDYRTILQEENDAVRERLDLSMERVAAMGEDDIRQPYGNYFRCV
                     SRFILMIGELVKERQEGRKLFLTQLQERNHRLYEDILPEHYEESFANPDYAVKELGEG
                     YGQILSALYTEIRGDIVYAFEMRLENIAILNEVFLEVYNLFVQAWEEGADCPKEQQIK
                     DAFYWYVSDYSDLMLPWRIREGLDPKLSFAKDIIMESDLNDLSYLYEFGEYISQSELK
                     LAAFMNTLPQETVDKMADTYTEGYRKGFSVMGRDITQKKTVVVEYQLGFERMIREAVK
                     NFRKMGLEPIFYRAAVESLNRRPNGKRGYFGASANKQYDYDHRYDSALYMGNAFKERK
                     LSILKAAYEQYSELASWCAGPAVVETFGEEGFTPVNKKTALALNDHQQELTLAYANES
                     RQVINQYMPGDETSFTIIAFPKPEIGP"
     gene            complement(1334..1549)
                     /locus_tag="ACZPCL_13775"
     CDS             complement(1334..1549)
                     /locus_tag="ACZPCL_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163570.1"
                     /translation="MYFSVKNAGQGGLNTGAYGFSSMAAAIMGLWYGILSFTEDDCNY
                     ILARIGTALEGIILLVWVIIIFTGIFR"
     gene            complement(1533..1655)
                     /locus_tag="ACZPCL_13780"
     CDS             complement(1533..1655)
                     /locus_tag="ACZPCL_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163571.1"
                     /translation="MRQIGNKVSYVKNPFAKNSFYCLGLGIASLLRCLKHVFFC"
     gene            complement(1652..2119)
                     /locus_tag="ACZPCL_13785"
     CDS             complement(1652..2119)
                     /locus_tag="ACZPCL_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR1163572.1"
                     /translation="MIRNTTKRQKMGWVLFILYLCLLAYFMFFSESFGRTDTDRDYAY
                     NLILFKEISRYFKYYNVLGFPLFMINIVGNIVAFAPFGFFLPVISRRSKKWYNTVTFG
                     FTWSLILETLQLISKVGSFDVDDLFLNTLGAAFGFGCYCLVQHMRLRYRRKIH"
BASE COUNT          628 a          540 c          406 g          637 t
ORIGIN      
        1 ggtccgatct ccggcttcgg gaaagcaata atagtaaagc tggtttcatc tcccggcata
       61 tactggttga tgacctggcg tgactcattt gcataagcta aagtcagctc ctgctgatga
      121 tcatttaaag caagggctgt tttcttattt accggtgtaa agccttcttc cccgaaggtc
      181 tctactacag cagggccggc acaccaggaa gccagttcac tgtactgctc ataagcagcc
      241 tttaaaatag aaagttttct ctctttaaag gcatttccca tataaagggc gctgtcataa
      301 cggtgatcat aatcatactg cttgttggca gaagcgccaa aatagcctcg ttttccgttt
      361 ggtctgcggt ttaaggactc taccgcagcc cgatagaaaa tcggttccag tcccattttc
      421 cggaaattct taacagcttc ccggatcatc cgttcaaagc ccagctggta ttctacaacc
      481 acagtcttct tttgggtaat atcccgtccc ataacagaaa aacctttacg ataaccttct
      541 gtataggtat ctgccatttt atccacagtt tcctggggaa gtgtgttcat aaaagccgcc
      601 agctttaact cagactggga aatatactca ccaaactcat ataaatagga aagatcattt
      661 aaatcacttt ccatgataat atcttttgca aaagaaagct tcggatccag gccttctctg
      721 atccgccacg gaagcatcag atctgaataa tcactgacat accagtaaaa agcatctttg
      781 atctgctgtt ctttagggca gtctgctcct tcttcccatg cctgtacaaa caggttatat
      841 acctccagaa agacttcgtt taagatagcg atattttcca gacgcatttc aaaagcataa
      901 acaatgtctc ccctgatctc tgtataaaga gcagacaata tctgtccata gccttctccc
      961 agctctttta ccgcatagtc cggatttgca aagctttctt cataatgttc cggcaaaata
     1021 tcttcgtaaa gcctgtgatt tcgctcctgt aattgggtga gaaaaagctt ccttccttcc
     1081 tgtctttctt ttaccagttc cccgatcatt aatataaagc gggaaacgca tctaaaataa
     1141 ttgccgtaag gctgccggat atcatcttct cccatggctg ccacgcgctc catggaaagg
     1201 tccagccgtt ctcttacggc atcgttttct tcctgtaaaa ttgttctgta atccataaaa
     1261 tgtattccct ccttgtacat ccgcatctgc ctgctttgat acttatattt tgcccttttg
     1321 atcctggtat cacttatctg aagataccgg taaatataat gatgacccag acaagaagga
     1381 tgatcccctc taaagccgtc ccgatcctgg ctaaaatata attacagtca tcttctgtaa
     1441 aggaaaggat gccgtaccag agccccataa tggctgctgc catactggaa aatccgtagg
     1501 ctcctgtatt taagcctccc tgtcctgcgt ttttaacaga aaaatacatg cttaaggcac
     1561 ctaagaaggg aggcgattcc cagacccaga cagtaaaaac tgttctttgc aaagggattt
     1621 ttcacatagg aaaccttgtt tccgatctgt ctcaatgtat tttcctcctg taacgtaatc
     1681 gcatatgctg taccagacag taacagccaa aaccgaaagc tgctcccagt gtattaagga
     1741 acaggtcatc tacatcaaaa ctgcccactt tggatatcag ctgcaatgtt tctaagatca
     1801 ggctccaggt aaaaccaaaa gtcactgtat tataccattt cttgcttctt ctgctgatga
     1861 ccggcagaaa aaatccaaag ggtgcaaaag caacgatatt tcccacaatg ttgatcataa
     1921 acaatggaaa tcccagtaca ttataatatt tgaaataacg gctgatctct ttaaaaagga
     1981 tcaggttata ggcgtagtcc ctgtctgtat ccgttcgtcc gaatgactct gaaaaaaaca
     2041 tgaaatacgc cagaaggcac aggtacagta taaacagtac ccatcccatc ttctggcgtt
     2101 tagttgtgtt tctgatcatc tgtgaccccg catcctaaaa ttcaatttcg gatttgcgtt
     2161 ttaaaagcag cgctccattg acaatggaaa taatgcctac cacaagacct g
//
LOCUS       DBMDUA010000313         2174 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_17134_length_2174_cov_2.212735, whole genome shotgun sequence.
ACCESSION   DBMDUA010000313 DBMDUA010000000
VERSION     DBMDUA010000313.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17134_length_2174_cov_2.212735"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="ACZPCL_13790"
     CDS             complement(<1..736)
                     /locus_tag="ACZPCL_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1163573.1"
                     /translation="MEQKNNAPFLAFTRICLNNWHYISRKILSLNKEINFFTGHSGSG
                     KSTVIDALQLVLYANTDGRGFFNKAAADDSDRSLIEYLRGMVNIGENNEFSYLRNQNF
                     SSTIVLEMQQTCTEEYQCIGVVFDVNTASNEVSRRFFWHKGPLWENEYRTPSRPMSIS
                     EVEQYLREHYSKEDYFSTSHNERFRRILYDSYLGGLDSEKFPLLFKRAIPFRMNIRLE
                     DFVKEYICMEQDIHIDDMQESVMQYGR"
     gene            complement(720..1418)
                     /locus_tag="ACZPCL_13795"
     CDS             complement(720..1418)
                     /locus_tag="ACZPCL_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HIR1163574.1"
                     /translation="MNDTSDLIPENETNWQIPYFDNLTEEQQKEITDSIRLLLRQTFV
                     LERKYDKKTERLQYTAAYRTISKHFPFIRHYLAVAGIELTENSHLGIIYVQGEDLLGE
                     KLPKLATLYLLALKLIYDEQMENVSTSVNVYTTLGEIQEKLGGYRLFKRQPAPTDIRR
                     TIALLRKYQIIEPLELLDDLNSSSPFMIYPCINVVLLGDDVRALLEDFDNGDTERIEP
                     DLAEEGGNEDGTEE"
     gene            complement(1411..>2174)
                     /locus_tag="ACZPCL_13800"
     CDS             complement(1411..>2174)
                     /locus_tag="ACZPCL_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Wadjet anti-phage system protein JetA family
                     protein"
                     /protein_id="HIR1163575.1"
                     /translation="VQEVVNKKYHILKTSDNFYLYKTDIKTWIRSMREDEQWQKRMAE
                     GMAPSMILQKLDQLERGFDDIEHRITNIDREHSRYVKATVTRLGYLLNQEDDMKGLVI
                     QLLNRLSANNGDEDMIQAVGSRMNLSQTGLLSEEALYKQRRPRTDFAKQLPDEKELPE
                     LSEEEILNYNKVKNRYSRREIEDFIQDHMENGTMKVDQNTVSSDEDFEKLILAYDYST
                     RTKSRYKAVESDAKPIKNGPYTYPKFQFVRRNTRE"
BASE COUNT          557 a          500 c          446 g          671 t
ORIGIN      
        1 tgcgcccgta ctgcatgacg ctctcctgca tgtcatcgat atggatatcc tgctccatgc
       61 agatgtattc cttcacgaaa tcttccaggc ggatgttcat acggaaagga atggcccgct
      121 taaaaagcaa tgggaacttt tcgctgtcaa gacctcctaa atagctgtca taaaggatcc
      181 tgcgaaatct ctcattatgg gaagtggaaa aataatcttc cttgctgtaa tgttccctta
      241 aatactgttc tacttcgctg atggacatag gccttgaagg tgtcctgtat tcattttccc
      301 ataaaggacc tttgtgccag aagaaacgtc tggaaacttc attggaagct gtgtttacat
      361 caaagacaac accgatacac tgatattctt ctgtgcaggt ctgctgcatt tccagtacaa
      421 tggtagaact gaaattctgg ttgcgcagat aggaaaattc attattttcc ccgatattta
      481 ccataccgcg cagatattcg atcaggctac ggtccgagtc atcagcagca gccttgttaa
      541 agaagccgcg gccgtctgta ttggcataaa ggaccagctg caatgcgtca ataaccgtag
      601 actttccgct tcctgagtga ccggtaaaga aattgatctc cttattcagg gaaaggatct
      661 ttctggaaat ataatgccag ttgtttaaac agatcctggt aaatgccaga aaaggagcat
      721 tattcttctg ttccatcttc gtttcctcct tcctctgcca gatcaggttc tatacgctct
      781 gtatccccat tatcaaaatc ctctaaaagg gcccgtacat catcccctaa aaggactaca
      841 ttaatacagg gataaatcat aaaaggactg gagctgttca gatcatctaa cagttccaaa
      901 ggctctatga tctgatattt tcttaacaat gctatggtgc gccggatatc cgtaggtgca
      961 ggctgacgtt taaacagacg gtaacctcct aacttctcct ggatttctcc cagagtcgta
     1021 taaacattta cactggtgga aacattttcc atctgttcgt catagatcag ctttaatgcc
     1081 agaagataaa gagtggccaa ttttggaagt ttttctccaa gcagatcctc tccctgtaca
     1141 taaatgatcc ctaaatggct gttttctgtc agctcgatcc cggcaacagc caggtaatgg
     1201 cggataaaag ggaaatgttt actgatggtg cggtaagctg ccgtatactg aagccgctct
     1261 gtctttttat cgtatttgcg ctccaataca aaagtctggc gcaaaagcag acggatggag
     1321 tcggtgatct ccttttgctg ttcttctgtc agattatcaa aataggggat ctgccagtta
     1381 gtttcattct ccgggatcag atcagatgta tcattcacgg gtattcctcc ttacaaattg
     1441 gaatttagga taagtataag gaccattctt tatgggcttt gcatcacttt ccactgcttt
     1501 ataacggctt ttggtccgtg tggaatagtc ataggcaagg atcagttttt caaaatcttc
     1561 gtccgaagat acggtattct gatctacttt catagtgcca ttttccatat gatcctggat
     1621 gaaatcttca atctcccgcc tgctgtaacg gttctttact ttgttgtaat ttaagatctc
     1681 ttcttccgaa agttccggca gttccttttc atccggcagc tgttttgcaa agtccgttct
     1741 tgggcggcgc tgcttataaa gagcttcttc tgacaacagg cctgtctggg aaaggttcat
     1801 acggctgcct accgcctgga tcatatcctc atctccgtta ttggcggaca gacggtttaa
     1861 gagctggatc accagccctt tcatatcgtc ttcctgattt aataaatagc ccagtcttgt
     1921 taccgtagct tttacataac gggaatgttc cctgtcaatg ttggtgatcc tgtgctcaat
     1981 gtcatcaaag ccccgttcta actgatccag tttttgaagg atcatagaag gggccattcc
     2041 ctctgccata cgtttctgcc actgttcatc ttcccgcatg gagcggatcc aggtcttaat
     2101 atccgtctta taaagataaa aattgtcaga agtctttaag atgtggtact ttttatttac
     2161 tacttcctgt acat
//
LOCUS       DBMDUA010000314         2150 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_17388_length_2150_cov_2.395803, whole genome shotgun sequence.
ACCESSION   DBMDUA010000314 DBMDUA010000000
VERSION     DBMDUA010000314.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17388_length_2150_cov_2.395803"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="ACZPCL_13805"
     CDS             <1..1038
                     /locus_tag="ACZPCL_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HIR1163576.1"
                     /translation="RSIGAEFPSGVNIVRTPEVPPAIRLFPSRQVAIIGNNGTGKTTL
                     LKIINGIIPADSGQIRLGSKVHIGYYDQEHQVLHMDKTLFQEIQDTYPNMNNTQIRNT
                     LAAFLFTGDDVFKLIRDLSGGERGRVSLAKLMLSDANFLLLDEPTNHLDITSKEILES
                     ALNRYTGTVLYVSHDRYFINRTATRILDLTDGSFINYIGNYDYYLEKKEDVEAAFAAR
                     QMAEAGNNRTAGANGTQAQSVSAVSGVSSASSQAPMTASAAGSLADGKMDWKAQKEEQ
                     ARLRKRQNELKKVEDKIHELETRDGEIDELLSQEDVYTDVSRLMELNKEKESIQKELE
                     VLYEKWEELAE"
     gene            1058..1285
                     /locus_tag="ACZPCL_13810"
     CDS             1058..1285
                     /locus_tag="ACZPCL_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163577.1"
                     /translation="MKKLKRISAALMVILLAGLFIAAIVLAITGAPANQLMAVIFSIV
                     FISVLFYAMGLMTRVLKKDDSKDTSDTSLKK"
     gene            complement(1450..>2150)
                     /locus_tag="ACZPCL_13815"
     CDS             complement(1450..>2150)
                     /locus_tag="ACZPCL_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1163578.1"
                     /translation="GLAAFWPAGATLDNFKEVFVRSNMPRYILNSVTYVTILVFLSMA
                     VNSAFGYALAKFEFKGKNVILTVVLSLLVLPLESILLSLFFIVNKLGWVDSYLALIIP
                     FIVKCFDVYLFRQFFLDVPDDLIEAAEIDGAGPFKTFLQVIIPVSKPVFATVFILDYV
                     AYWSDFMWPLLVITDAGKRTVQLGIQTFFTEPPVYYGPIMAALTIASVPMIIMFLFLQ
                     KYYVQGISTSGIKG"
BASE COUNT          676 a          471 c          453 g          550 t
ORIGIN      
        1 cgaagcatcg gtgcggagtt tccttcggga gtgaatatag taagaacacc ggaagtgccg
       61 ccggctatcc ggctatttcc ttcccggcaa gtagcaatca ttggaaataa tggtaccgga
      121 aagaccactc ttttaaagat catcaatggc attatccctg ctgattccgg tcagatccgt
      181 ttaggttcca aggtgcatat cggctactat gatcaggagc atcaggtact gcatatggat
      241 aaaaccctgt tccaggagat ccaggatacc tatccaaaca tgaacaacac ccagatccgc
      301 aatacactgg ctgctttcct gtttacagga gatgacgtat ttaagctgat ccgtgactta
      361 agcggcggtg aaagaggccg tgtttccctt gcgaaattaa tgctctctga tgccaatttc
      421 ctgcttcttg acgagcctac caaccatctg gatattactt ccaaggagat cctggaaagc
      481 gcattaaacc gctatacagg aacggtattg tacgtatccc atgaccgtta tttcatcaac
      541 cgtactgcta cccgtatcct ggatctgacg gatggttcat ttatcaacta tatcggcaat
      601 tatgactact atctggaaaa gaaagaggat gtagaagctg catttgccgc taggcagatg
      661 gctgaagctg gaaataaccg tacagccggt gcaaatggca cacaggcaca gagtgtcagc
      721 gctgtttcag gcgtatcttc cgcttcttcc caggcaccta tgaccgcctc cgctgcaggc
      781 tctttagctg acggaaaaat ggactggaaa gcccagaaag aggagcaggc ccgtcttcgc
      841 aaacgccaga atgaactgaa aaaggtggag gacaagatcc acgaactgga aacaagagac
      901 ggggagatcg atgagctcct ctcccaggaa gatgtatata ctgatgtttc ccgccttatg
      961 gaactgaaca aagagaaaga gtccatccag aaagaactgg aagttctcta tgagaaatgg
     1021 gaagaactgg cagaataaaa aggagttata aaaatccatg aagaaattaa aacggatatc
     1081 agctgccctt atggtaatat tattagcagg cctttttatt gcagccattg tacttgcaat
     1141 aaccggcgcc ccggccaatc agctgatggc agttattttc tctatcgtgt ttatttccgt
     1201 attgttttat gctatgggac tgatgacacg ggtgttaaaa aaagatgaca gtaaggatac
     1261 atctgataca tcattaaaaa aatagccagc ctctcgtctg caatgccggg ccagttattt
     1321 atgaaaaagt gtaccagatc ctgaatttat ttataatttt taatgcaaaa ggtctgcttt
     1381 tgataaaata tcatcagcag accttttttt atattttttg actttttaat attatcaacc
     1441 ttgatattgt caacctttaa taccactggt agaaattccc tgcacataat atttctgtaa
     1501 aaacaggaac ataatgatca taggcacaga tgcaatagtc agcgcagcca tgataggacc
     1561 ataataaacc ggaggttctg taaaaaatgt ctgaatacca agctgtaccg tcctttttcc
     1621 tgcatcagtg ataacaagca gaggccacat aaagtcggac cagtaagcca cataatccag
     1681 aataaagaca gtagcaaaaa caggcttgga taccggtatg atgacctgta aaaaggtttt
     1741 aaatggtccg gctccgtcaa tctctgctgc ttcgatcaga tcatccggca catccaggaa
     1801 aaactgcctg aacaggtata cgtcaaaaca ttttacaata aaaggaatga tcagtgccag
     1861 ataggaatcc acccatccca gcttatttac aatgaaaaac aaagatagca aaatactttc
     1921 caacggcagt accagcaaag ataataccac tgtaaggatc acatttttac ccttaaattc
     1981 aaattttgca agtgcataac caaaggcaga gtttactgcc atgcttaaaa acacaaggat
     2041 cgtaacataa gtaacactgt tcaggatata gcggggcatg ttgcttcgta caaagacttc
     2101 tttaaaatta tctaaggtcg ctcctgcagg ccagaaggct gccagtcctt
//
LOCUS       DBMDUA010000315         2142 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_17462_length_2142_cov_2.572268, whole genome shotgun sequence.
ACCESSION   DBMDUA010000315 DBMDUA010000000
VERSION     DBMDUA010000315.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17462_length_2142_cov_2.572268"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="ACZPCL_13820"
     CDS             <1..629
                     /locus_tag="ACZPCL_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR1163579.1"
                     /translation="DGFTHYEQSVRVVEKLEKNGKGLNLTWEVRDGIRNHRTAGHPST
                     LEGGVVRLSDKIAYINHDIDDAIRAKMFEEKDLPVCYTDVLGHSVRERLNIMIHDIIL
                     NSLDKPGIFMSEGMEEAMMGLRQWMFDHVYKNEIPKAEEGRAQQMLSQLYFYYMDHVD
                     ELPQEYLNFLDAGEKVSRVVCDYIAGMSDIYAIDQFEKLFVPKRWNVY"
     gene            684..>2142
                     /gene="dnaG"
                     /locus_tag="ACZPCL_13825"
     CDS             684..>2142
                     /gene="dnaG"
                     /locus_tag="ACZPCL_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR1163580.1"
                     /translation="MYYPDEVIEEVRMKNDIVDVISGYVKLQKKGANYFGLCPFHNEK
                     SPSFSVSPGKQMYYCFGCGAGGNVLTFVMEYENYTFQEALQSLADRAGVTLPKMEYSK
                     EAREQAEFRARLLEVNKLAANYFYYQMKQPQGKIAYEYFHDKRKLTDETMLRFGLGYS
                     NKTSDDLYRFLKEKGYDDAFLSQTGLVTIEERGGRDKFWNRVMFPIMDVNNRVIGFGG
                     RVMGDGEPKYLNSPETKLFDKSRNLYGLNYARTTREKYMLVCEGYLDVISMHQAGFTN
                     AVASLGTAFTSQHAGVLKRYTDQVILTYDSDGAGIKAALRAIPILRDAGISARVLNMK
                     PYKDPDEFIKNMGADAFKERIAQAKNSFLFEIDVLKRNYQLEDPEQKTKFYQETAKKL
                     LQFGEPLERDNYIQAVSREQMIKEEELRQLVNRLGMQMGLKAGDSYREDASGRNVISR
                     ENGSGPGNDMGRPEYGGNPYEGQAALPGKGHWGSVP"
BASE COUNT          652 a          407 c          564 g          519 t
ORIGIN      
        1 aagatggctt tacccattat gagcaaagtg tacgggtggt ggagaaactg gaaaaaaatg
       61 gaaaaggatt aaaccttacc tgggaagtgc gggatgggat acgaaatcac cgtacagccg
      121 gtcatccttc taccttagaa ggcggcgtgg tccgtctctc tgataagatc gcctacatca
      181 accacgatat agatgatgcc atccgggcga agatgtttga ggagaaagac cttcctgtct
      241 gctatacaga tgtgttaggc cacagcgtcc gggagcgttt aaatatcatg atccacgata
      301 ttatcttaaa cagtctggat aagccgggga tctttatgtc agaaggcatg gaagaggcca
      361 tgatgggtct tcgtcagtgg atgtttgacc atgtatataa aaatgagatc ccaaaagcag
      421 aagagggaag agcccagcag atgctttccc agctttattt ctactatatg gatcatgtag
      481 atgagctgcc acaggagtat ttaaacttct tagatgcggg agaaaaagtc tcgcgggtgg
      541 tttgcgacta catagcaggc atgagtgata tctatgccat tgaccagttt gaaaaattat
      601 ttgttccaaa acgctggaac gtatattaaa acaggggcaa tgtttttgtg cccgatatta
      661 ctttaacaga agggagaaaa agcatgtact atccggatga agtcatagag gaagtaagaa
      721 tgaagaatga catcgtagat gtgatttcag gatatgtaaa gctgcagaaa aaaggagcca
      781 attattttgg cctttgtcct ttccataatg aaaaatcacc ttctttttcc gtttctccgg
      841 gaaaacagat gtattactgc tttggctgcg gagctggagg aaatgttctt acctttgtaa
      901 tggaatatga aaactatacc tttcaggaag cccttcagtc cctagcagac cgggcgggag
      961 ttactcttcc aaagatggaa tacagcaaag aagccaggga gcaggcggaa tttcgtgcca
     1021 gactgctgga ggtaaataag ctggcagcca attatttcta ctaccagatg aagcagccac
     1081 agggaaaaat cgcatatgaa tatttccatg acaagcgaaa gcttacagat gagaccatgc
     1141 tccggtttgg ccttggctat tctaataaga ccagtgatga tctgtaccgt ttcctgaaag
     1201 aaaaaggcta tgatgatgca tttttaagcc agacaggcct tgtgaccatt gaagagagag
     1261 gcggaagaga taaattctgg aaccgtgtta tgttcccaat catggatgtg aacaaccgtg
     1321 tcatcggttt tggcggccgt gtcatgggag atggggagcc gaaatattta aactcaccgg
     1381 agacaaaatt atttgataaa agccggaatc tgtatggttt aaactatgca aggaccacca
     1441 gggaaaaata catgctggta tgtgaaggct acctggatgt gatctccatg catcaggcgg
     1501 gctttaccaa cgcagtggca tctttgggaa cagcctttac cagccagcat gcaggtgttt
     1561 taaagcggta tacggaccag gtgatcttaa cctacgacag tgacggtgcc ggtatcaagg
     1621 ctgcgctgcg tgccattcct atattaaggg atgcaggtat ttctgccaga gttttaaata
     1681 tgaagccata caaggacccg gatgaattta ttaaaaatat gggggctgat gcctttaagg
     1741 agcggatcgc ccaggcaaag aacagcttcc tatttgaaat agatgtatta aaacggaact
     1801 atcaattaga agatccggag cagaagacga aattttatca ggaaacagca aagaagcttt
     1861 tacagtttgg agaaccgctg gagcgggata attacatcca ggcagtatcc agggagcaga
     1921 tgatcaaaga agaggagctt cgccagctgg taaaccgtct gggaatgcag atgggcttaa
     1981 aagccggtga cagctacagg gaagatgctt ccggaagaaa tgttatttcc agggaaaatg
     2041 gaagcggtcc aggtaatgat atgggccggc cggaatatgg cggcaatcct tatgagggac
     2101 aggcagcgct gccagggaaa ggacactggg gatcagtgcc ag
//
LOCUS       DBMDUA010000316         2080 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_18122_length_2080_cov_3.277413, whole genome shotgun sequence.
ACCESSION   DBMDUA010000316 DBMDUA010000000
VERSION     DBMDUA010000316.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18122_length_2080_cov_3.277413"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="ACZPCL_13830"
     CDS             complement(<1..1107)
                     /locus_tag="ACZPCL_13830"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1163581.1"
                     /translation="MKRKTWIVSALMYALVFLVCFGVLHVFKKITTAGDSTEYTSKAA
                     AETNIVIDGSADDTDRENGKMDGAEGSDLSEDTDELWNETEKNTHIRRETGYKGWSKT
                     LPQVWPEVPEEEHYTPPRIIQATDVHYQSADAEDDGAAFQLFVERCDGKVVKYLPQLL
                     DAFMDQVIAEKPTALVLSGDITMNGEKINHKELAEKLHKVQDAGIPVLIIPGNHDINN
                     PNAAVYFGDEKTSTDSVTPEEFYDIYHMYGYDQAISRDSASLSYVYQLDERNRLLMLD
                     SCQYEPKNLVEGRIKAETLVWMDEQLKKAQEDGMQILPIAHHNLLAQSRMYTTQCAMD
                     NNGEVIDLLQKYKIPLFLSGHLHVQRIRKHKAEPG"
     gene            complement(1111..1923)
                     /locus_tag="ACZPCL_13835"
     CDS             complement(1111..1923)
                     /locus_tag="ACZPCL_13835"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1163582.1"
                     /translation="MFSEMMNNNRTKALFFDIDGTLLSEKTRRVPDSAKEALKQAREK
                     GHLVLINTGRVYPHLHEIRSQVDADGFLCGCGTYVRVKDEVLYHHVIPHERGLQIKKD
                     IDDCGLDGVLEGLGGCYMHEAPSCLPQVERVKQSVKLSGCPMPCYWEDDSFDFDKFYI
                     GSDEKSRCKELFGRMPDIEVIDREKGFYECIPRGHSKATAIDLVLKHYGISKEDAYVF
                     GDSSNDLSMFQYAVNCIAMGDHSPVLEPYISFVTKTVEDDGIAYAMKELGII"
     gene            complement(1901..>2080)
                     /locus_tag="ACZPCL_13840"
     CDS             complement(1901..>2080)
                     /locus_tag="ACZPCL_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163583.1"
                     /translation="VTNKFWDNWFISIGAGIVIEKGFQVGGDLIRSEGIRLESLAIVD
                     SMDEETGTIVFRDDE"
BASE COUNT          521 a          542 c          399 g          618 t
ORIGIN      
        1 tcccggttct gccttgtgct tgcggatcct ctgaacatga agatgaccgc ttaaaaacag
       61 ggggatctta tatttctgaa gaagatcaat gacctcccca ttattgtcca tggcacactg
      121 ggttgtatac atacggctct gggccagcag gttgtgatgg gcaataggca atatctgcat
      181 cccgtcttcc tgggcctttt ttaactgctc atccatccac accaaggtct ctgccttgat
      241 ccggccttct accaggttct taggctcata ctggcaggag tctaacatta aaagacggtt
      301 tctttcatct agctggtaaa catagcttaa agaagcactg tcccggctga tggcctgatc
      361 gtaaccgtac atatggtaaa tatcataaaa ttcttccggg gtaacgctgt ctgtggatgt
      421 tttctcatct ccaaaataaa cagccgcatt gggattattg atatcatggt ttccagggat
      481 gatcagtacc ggaatacctg catcctgaac tttgtgaagc ttctcagcca gttccttatg
      541 attgatcttc tccccgttca tggtaatatc accactgagt accagtgctg ttggtttttc
      601 agcgatcacc tgatccataa aagcatctaa cagctgtgga agatatttta ccacttttcc
      661 gtcgcagcgc tctacaaaaa gctgaaaagc tgctccgtca tcctctgcat ctgcgctctg
      721 ataatgaaca tccgttgcct ggatgatcct tggaggcgta taatgctctt cttccgggac
      781 ctccggccaa acctgaggca atgtcttact ccagccttta tagcctgttt cccgtcggat
      841 atgggtattt ttctccgtct cattccataa ttcatctgta tcttctgaca gatctgatcc
      901 ttcagccccg tccatttttc cattttcacg gtctgtatca tctgcagacc cgtcaattac
      961 aatattggtc tctgccgcag ccttagaggt gtactccgta ctgtctccgg cagttgttat
     1021 tttcttaaaa acgtgcaata ccccgaagca taccagaaat acaagggcat acatcagggc
     1081 actgacgatc caggtttttc gtttcataat ttatatgatc cccagctctt tcatagcata
     1141 ggcgatcccg tcatcctcta ctgtttttgt tacaaaagat atatatggtt ccaacaccgg
     1201 actgtgatct cccatggcga tacagttgac tgcatactgg aacatggaca gatcattgct
     1261 gctgtcacca aatacataag catcctcttt tgaaatgcca tagtgtttta acacaagatc
     1321 aatggcagtt gccttggaat gacctcttgg gatacactca taaaagccct tttccctgtc
     1381 aatgacctca atatccggca ttcttccaaa cagctctttg cagcggctct tttcatcaga
     1441 accaatgtaa aacttgtcaa aatcaaaact gtcatcttcc cagtagcatg gcattggaca
     1501 gccggagagc ttaacagact gttttacacg ctctacctgc ggcagacagg atggtgcttc
     1561 atgcatatag cagccgccca gaccttccag tacaccgtcc agaccgcagt catcaatgtc
     1621 ttttttgatc tgcagtcctc tttcatgtgg gatcacatgg tgatataaga cctcatcttt
     1681 tactctcaca taagtaccgc agccgcagag aaatccatct gcatctacct gggaacggat
     1741 ctcatgtaaa tgcggataca cacggcctgt attgatcagg accagatgtc ctttttctct
     1801 tgcctgtttc agtgcctctt tcgcactgtc aggaaccctc cgcgtctttt cagacaggag
     1861 ggttccgtca atatcaaaaa ataaagcttt tgttctgtta ttattcatca tctctgaaaa
     1921 caatggttcc tgtctcctca tccatactgt caacaatagc cagtgactcc agacggattc
     1981 cttcggaacg gatcaaatct ccgcctacct ggaagccttt ttcaataacg ataccagcac
     2041 ctattgaaat aaaccagtta tcccaaaatt tattggtaac
//
LOCUS       DBMDUA010000317         2071 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_18240_length_2071_cov_2.355330, whole genome shotgun sequence.
ACCESSION   DBMDUA010000317 DBMDUA010000000
VERSION     DBMDUA010000317.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18240_length_2071_cov_2.355330"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /gene="minD"
                     /locus_tag="ACZPCL_13845"
     CDS             <1..706
                     /gene="minD"
                     /locus_tag="ACZPCL_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HIR1163584.1"
                     /translation="LGKRVVLIDTDIGLRNLDVVMGLENRIVYNLVDVAEGNCRMKQA
                     LIRDKRYPNLYLLPAAQTRDKSAVTPEQMIKLTDDLKDDFDYILLDCPAGIEQGFYNA
                     IAGADRALVVTTPEVSAIRDADRIIGLLEHAQIGAIDLIVNRIRADMVRRGDMMSLTD
                     VQDILAVNMIGAIPDDENIVVSTNQGEPLVGMGSAAGQAYLNVCRRLLGQNVPMESPG
                     ETAGILAKLSRILRRA"
     gene            720..983
                     /locus_tag="ACZPCL_13850"
     CDS             720..983
                     /locus_tag="ACZPCL_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HIR1163585.1"
                     /translation="MRLRCSKDTAKLRLTFLLLSDKAGCSPEMIAAIKNDMIRVISKY
                     MKIEKDAVRLTMDAGSFSDGQIYHLPVLRADIPIRSMSSKGLY"
     gene            985..>2071
                     /locus_tag="ACZPCL_13855"
     CDS             985..>2071
                     /locus_tag="ACZPCL_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1163586.1"
                     /translation="MFFDYDFRNYNFRLMAYMIALNVIGVLVVRSATNKDPDFVTKQL
                     MGVMVGLVIAICLSLIDYHKILNFSTIIYLLCISSLIAVLIWGKSVNNAKRWIEVPVL
                     GQLQPSEFVKIGLIITFSWYFMKYQEKINNVSTVAIAAALFAVPAMLIFEQPNLSTCL
                     VTLVIVLGIVFSAGISYRWIVGVLAVTIPMVGTCVYLLLHGLIPFIKEYQAGRILAWF
                     YPDRYGEARYQQNNSIIAIGSGQLKGKGLFNTTIASVKNGNFLSEEQTDFIFAVIGEE
                     LGFIGCVIVIILFLLIVYECLMMAARAKDLAGRLICTGMATLIAFQGFANIAVATGIF
                     PNTGLPLPFISYGSSSLISIFIGMGLVL"
BASE COUNT          545 a          449 c          498 g          579 t
ORIGIN      
        1 tctgggaaaa agggtcgttc tgatcgatac agatatcggc cttagaaacc tggacgtagt
       61 tatgggactg gaaaaccgca ttgtctacaa tctggtagac gttgcagaag gaaactgccg
      121 tatgaagcag gccctgataa gagacaaaag atatccaaac ctttaccttc tgccagctgc
      181 acagacaaga gataaatccg ctgtgacacc tgagcagatg atcaaactca ccgatgacct
      241 gaaagacgat ttcgactata tcctgttgga ttgtcccgca ggcattgagc agggttttta
      301 caatgccatt gccggtgcag accgggcact ggtagtcacc acacctgaag tttcggcgat
      361 ccgggatgcg gaccgtatca ttggtctgtt agaacacgcc cagatcggtg ccattgacct
      421 gatcgtaaac aggatccgtg cagatatggt acgccgggga gatatgatgt cattaaccga
      481 tgtacaggat atcctggcgg tgaatatgat cggagccatt cccgatgacg aaaacatcgt
      541 ggtctccaca aaccaggggg aacctttagt gggtatggga tctgccgcag gacaggctta
      601 tttaaatgta tgcagacgcc tgcttggaca gaatgttccc atggagtctc cgggagaaac
      661 agccggtatc ctggcaaaac tttccaggat cttaagaagg gcataagggg tgatggcagt
      721 tgagattacg gtgttccaaa gataccgcaa agctccggct tacctttctt cttttatctg
      781 ataaagcagg atgttcaccg gagatgatag cagcgataaa aaatgacatg atccgtgtca
      841 tttccaaata catgaagata gaaaaagacg cagtgcgtct taccatggat gcaggcagtt
      901 tttcagatgg tcagatctat cacctgcctg tactccgtgc ggatataccg atacggtcca
      961 tgtccagcaa ggggctatac taatatgttt tttgattacg actttagaaa ttataacttt
     1021 cgtcttatgg cctatatgat cgccctgaat gtgatcggcg tcctggtagt acgcagtgct
     1081 accaataagg atcctgattt tgttaccaaa cagctgatgg gtgttatggt aggtcttgtg
     1141 atcgccattt gtctttctct gatcgattac cataagatat taaacttcag tacgatcatt
     1201 tatctgctgt gtatctccag tctgatcgca gtactgatct ggggcaaatc tgtcaacaac
     1261 gcaaaacgct ggatcgaagt tccggtactt ggacagcttc agccttccga atttgtaaaa
     1321 attggtctga ttattacttt ttcctggtat tttatgaaat atcaggaaaa aataaataat
     1381 gtaagcacag ttgccattgc agcagcgctt tttgcagtac cggctatgct catttttgag
     1441 cagccaaacc tttccacctg cctagttaca ctggtgatcg tgctgggaat cgtcttttca
     1501 gcaggcatca gctaccggtg gatcgtggga gttttagctg taaccatccc tatggtggga
     1561 acctgtgtct atctgctttt acacggtctg atccctttta tcaaagagta tcaggcaggc
     1621 agaattttag catggttcta tcctgacaga tacggggaag cacgttacca gcagaataac
     1681 tccatcatcg ccattggctc cggacagtta aaaggtaagg gactgtttaa tacaacgatc
     1741 gcctctgtta aaaacggaaa tttcctttca gaagagcaga cagactttat ctttgccgtg
     1801 atcggtgaag aacttggttt tattggctgt gtgatcgtga tcattctgtt tttactgatc
     1861 gtttatgagt gccttatgat ggcagccaga gcaaaagacc tggcaggaag actgatctgt
     1921 acaggtatgg ccaccctgat cgcttttcag ggctttgcaa atattgcagt tgcaaccggc
     1981 attttcccta ataccggact tccccttccg tttatcagtt acggaagcag ttcgcttatc
     2041 agtattttta ttggtatggg actggtgcta a
//
LOCUS       DBMDUA010000318         2067 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_18290_length_2067_cov_2.606307, whole genome shotgun sequence.
ACCESSION   DBMDUA010000318 DBMDUA010000000
VERSION     DBMDUA010000318.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18290_length_2067_cov_2.606307"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="ACZPCL_13860"
     CDS             complement(<1..1228)
                     /locus_tag="ACZPCL_13860"
                     /inference="COORDINATES: protein motif:HMM:NF021445.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HIR1163587.1"
                     /translation="MSRQKKIQLENGTILFLGAAAFILLAASLFPSSAMSQKVRSYLE
                     MNRMVERAFAVMLLVLSTQLMKRKRTAWEMTMAVLTLSLLRGLEEIIHTGLEARSLFL
                     LVQAAVFFILLWSRKDFCCPASKKSVEQAVGFILLSLLGVAVNAAITRHYINLALGKG
                     TVSFVESLAAGVGMLFGIGGEFPGGEFAEHLEQLMFLFSWGCILAGAVHIFRPWMEYP
                     GKTASDLQHARTLLNLYSQNPCSYLTLEDDKILYFGKQVDGVIPYGIVGDTVVVNGDP
                     VCKDEDFPKLLDEFKEFCLKSAHKLFILSVTDHYLDEYKKQGFGTVKCGEEARFKLAD
                     YEISGKKGAKMRMNINHAKKAGIDVYEYKILEKRNAALEVEFNRITDEWLEGKKSSML
                     KFTMGTLGLDDPMDRRY"
     gene            complement(1246..>2067)
                     /locus_tag="ACZPCL_13865"
     CDS             complement(1246..>2067)
                     /locus_tag="ACZPCL_13865"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HIR1163588.1"
                     /translation="EVRRYGADTSKVVIGGSRLGIYFFEKGTDIRPTNVVYDREGSAL
                     ALAKPEEFHWDEILKDTDIFYFSGVTPAVSENIAKAVLEALKYCKAHGIETVCDLNYR
                     SKMWDTKKAQSVMSELMNYVTLCIANDEDFESSLGIHAYDGDISKGIDQIESYKAGMK
                     EIQNRYPNCKAVASVLRNMYTAEDGDWMAIYLKDGVFYESPVHKVHSFEAVGAGDAFA
                     GALLHSLVRGFEPQKLIDFSISASVLKLMIQRDFNLVTENEIMRVMESKAANLQR"
BASE COUNT          530 a          549 c          390 g          598 t
ORIGIN      
        1 aataccgccg gtccatagga tcatccagtc ccagagtacc catggtaaat tttaacatgg
       61 aacttttctt accttccagc cactcatcgg taatgcggtt aaattctacc tctaaagctg
      121 cattcctctt ctccagtatc ttatactcgt aaacatcgat ccccgctttt ttcgcatgat
      181 tgatgttcat acgcatcttg gcgccttttt taccgctgat ctcatagtca gccagcttaa
      241 atctcgcctc ttccccgcat tttacagttc caaagccctg ttttttatac tcgtccagat
      301 aatggtctgt cacacttaag ataaacagct tgtgggcgct ttttaagcag aattccttaa
      361 attcatccag taacttggga aagtcctcat ctttacaaac cgggtcaccg ttgactacca
      421 cagtatcgcc tacgatccca taggggataa cgccgtccac ctgctttcca aaatagagga
      481 tcttatcatc ttctaaggtc agataggagc agggattttg gctgtaaagg tttaataagg
      541 tcctggcatg ctgcaggtca gatgctgttt tcccgggata ttccatccac ggacggaata
      601 tatggactgc cccggccagg atacagcccc agctgaacag aaacatcagc tgctctaaat
      661 gctccgcaaa ctctcctcct ggaaattcac cgcctatgcc aaaaagcatt cccacaccag
      721 ctgcaaggct ttcaacaaaa gagaccgttc cttttcccag agccagattt atataatggc
      781 gggtgatggc tgcgttgaca gctactccca acagggataa gaggataaag ccaacagcct
      841 gttccaccga ctttttagag gccggacagc aaaaatcttt tctgctccac aaaaggatga
      901 aaaagacagc cgcctggacc agcaggaata aacttcgggc ctccagtcct gtatgtatta
      961 tctcttccag tccccgcaga agacttaaag tcagtacagc catggtcatt tcccaggccg
     1021 tcctctttcg cttcatcagc tgggtggaaa ggaccagcag catcactgca aaagctctct
     1081 ccaccatccg gttcatttcc agataagagc gcaccttttg tgacatggca gaagaaggaa
     1141 acagactggc tgccagtaag ataaaggctg ctgctcctaa aaataatatg gttccatttt
     1201 ctagttgtat ttttttctgt ctgctcatag aagccaccct caaaatcacc tttgcaggtt
     1261 tgccgcttta gactccataa ctctcatgat ttcattttcc gttaccaggt taaagtctct
     1321 ctggatcatc agttttaata cactggctga aatagagaaa tcaatcagtt tctgaggttc
     1381 aaatcctctt accagactgt gtaacagcgc tcctgcaaaa gcatctcctg ctcctactgc
     1441 ctcaaagctg tgtactttat gtaccgggct ttcatagaac acaccatctt ttaaatagat
     1501 cgccatccag tcaccgtctt ctgctgtata catgttgcga aggacgctgg caactgcctt
     1561 gcagtttgga taacggttct ggatctcttt catgcctgcc ttgtagcttt caatctgatc
     1621 aattcccttt gaaatatccc cgtcatatgc gtggatccct aaagatgact caaaatcctc
     1681 atcattggca atgcataatg ttacataatt catcagctcg ctcataactg actgtgcctt
     1741 ttttgtatcc cacattttag agcggtaatt taagtcgcag actgtctcga tcccgtgggc
     1801 tttgcagtat tttaaggctt ctaatactgc ctttgctata ttctcgctga ctgccggtgt
     1861 cacaccggaa aaatagaaaa tatctgtatc ctttaagatc tcatcccagt ggaattcttc
     1921 cggctttgcc aacgccagag cgcttccttc cctgtcatag actacattgg taggccgaat
     1981 atctgttcct ttttcaaaga aataaattcc cagtctggaa cctccgatga ctaccttgga
     2041 agtgtctgcc ccgtatcttc tcacttc
//
LOCUS       DBMDUA010000319         2055 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_18431_length_2055_cov_1.686796, whole genome shotgun sequence.
ACCESSION   DBMDUA010000319 DBMDUA010000000
VERSION     DBMDUA010000319.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18431_length_2055_cov_1.686796"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(68..535)
                     /locus_tag="ACZPCL_13870"
                     /pseudo
     CDS             complement(68..535)
                     /locus_tag="ACZPCL_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595576.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(617..1132)
                     /locus_tag="ACZPCL_13875"
     CDS             complement(617..1132)
                     /locus_tag="ACZPCL_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HIR1163589.1"
                     /translation="MSLLGKLMDTMRLSSEDDEDDYYLDDDFEDEAPKKGLFSKRNNT
                     EPEDEPEGQEKPKFLGRSNPKVVPMRRSMEVTMIKPTSMDDSRDICDYLLSGKAVVLN
                     MEGIHMETAQRIIDFTSGATYSMNGNLQKISNYIFIATPDSVELSGDFQDILSAGVSS
                     GVDMSSLNIHL"
     gene            complement(1164..1862)
                     /locus_tag="ACZPCL_13880"
     CDS             complement(1164..1862)
                     /locus_tag="ACZPCL_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR1163590.1"
                     /translation="MIKEQLEEVRKNIEEACKRAGRDPKEVTLIAVSKTKPVPMLEEA
                     YEAGARDFGENKVQEIVQKKPELPEDIRWHMIGHLQRNKVHQVMGKAVLIHSVDSLRL
                     AEQIETEAAKKDMVADILLEVNVAKEESKYGFFLEDTEAAIREIAKFPHVRIKGLMTI
                     APFVENPEENREVFKKLYEFAVDIGKKNIDNVTMDVLSMGMTGDYQVAIEEGATMVRV
                     GTGIFGARLYGAAQ"
     gene            complement(1893..>2055)
                     /locus_tag="ACZPCL_13885"
     CDS             complement(1893..>2055)
                     /locus_tag="ACZPCL_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HIR1163591.1"
                     /translation="NINKGYTVFTQVEILDSNSEYYTIKNNVTYGLSVYDHIVLDASS
                     VGEGQLLYQ"
BASE COUNT          525 a          480 c          373 g          677 t
ORIGIN      
        1 tgagccggaa aatgctttcg ttgccgactt tatcggtgag agcaacattg tacctggcct
       61 tatgatccat gtttttgtag gcatcaaaat caacgcctgt ttttccaccg atactggaat
      121 ctacctggga aagcagggtc gtagggatct ggataaaatc aatgcccctt aactaggtag
      181 cagccgtaaa gccacacata tcgccgatca caccgccgcc taaagcagct aacatatcct
      241 tacggtcaaa atgctctaaa atgagatgtt catagatctt ttttactgta tccagatttt
      301 tattctcttc tccagccggg ataacaaaat aagagatctt cttacagcat ttgcttaaac
      361 gttcttttac ttcttccaga tataaaggag ccacattggt atctgtaaca atacagatct
      421 tgcgctcaga cagatggaat ttatttacct cttctgtcag cttttcaaaa gatggttcca
      481 ttacaatatc gtagatcggt ttctggtctt tatatacatt caggcgtttt gacatatcat
      541 tctccttagt cctgtttttc gtatttttac atagaaaatc ggcagactca tcaggggtct
      601 gccgcatgct ttggtattat aaatggatgt tcaggctgga catgtccact cctgagctga
      661 cacctgctga caggatgtcc tgaaaatcac cggacagttc cacggaatca ggtgttgcaa
      721 tgaagatgta attggaaatc ttctggagat ttccgttcat ggaataagtt gctccggaag
      781 tgaaatcaat gatccgctgc gcggtttcca tatgaattcc ttccatatta agtacaactg
      841 cttttccaga gagaagataa tcacagatat ctctggagtc gtccatagaa gtaggtttga
      901 tcattgtcac ttccatactt cttctcatag gaactacttt tggatttgaa cggcctaaaa
      961 attttggctt ttcctgtccc tctggctcat cttccggttc tgtattattt ctctttgaga
     1021 aaagtccttt cttaggagct tcatcttcaa agtcatcatc caggtagtaa tcgtcctcgt
     1081 catcttcaga acttaaccgc atggtatcca ttaacttacc taacagactc attttactct
     1141 ctccaatcct cttttcatgc tgcttactgg gcagcaccat atagccgggc accgaaaatg
     1201 ccggttccga ctcttaccat ggtagcgcct tcttcaatag caacctgata atctccggtc
     1261 atacccattg aaagcacatc catagtaaca ttatcgatgt ttttcttacc tatgtcaaca
     1321 gcaaattcat ataatttttt aaaaacttct cgattttcct ctggattctc tacaaaaggc
     1381 gcaattgtca taagcccctt aatacgtaca tgggggaatt ttgcgatctc ccggatcgct
     1441 gcttctgtat cttccaggaa aaagccgtat ttgctctctt cttttgccac attgacttcc
     1501 agtaaaatgt cagcaaccat atcttttttt gctgcttctg tttcgatctg ttcagccagg
     1561 cgtaaagagt ctacagaatg gatcaggact gcttttccca taacctgatg tactttgttg
     1621 cgctgtaagt gaccgatcat atgccagcgg atatcttccg gaagttccgg ctttttctga
     1681 acgatctcct gtactttatt ttcgccaaaa tccctggcac ctgcctcata tgcttcttcc
     1741 agcataggta ccggtttggt cttgctcact gcgatcagag tgacctcttt tggatcacgt
     1801 ccagcccgtt tgcaggcttc ttctatattc tttcttactt cttctaactg ttctttaatc
     1861 atggtctgtt cctccttttt ataatagaaa aattactgat aaagaagctg tccctctcct
     1921 acagaggacg cgtctaatac gatatgatca taaacagaaa gcccataagt tacattgttt
     1981 ttaatggtat aatactcact gttggagtcc aatatctcta cctgggtgaa gacggtatat
     2041 cctttattaa tattg
//
LOCUS       DBMDUA010000320         2039 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_18613_length_2039_cov_2.825593, whole genome shotgun sequence.
ACCESSION   DBMDUA010000320 DBMDUA010000000
VERSION     DBMDUA010000320.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18613_length_2039_cov_2.825593"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2039)
                     /locus_tag="ACZPCL_13890"
     CDS             complement(<1..>2039)
                     /locus_tag="ACZPCL_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009445460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HIR1163592.1"
                     /translation="EQESVKVEVLTGKKADKEAAKKGTIAYSILSAHNTADNMEHLKI
                     RFDSMASHDITFVGIVQTAKASGMEKFPIPYVLTNCHNTLCAVGGTINEDDHMFGLSA
                     AKKYGGIYVPPHIAVIHQYMREMMAGCGKMILGSDSHTRYGALGTMAIGEGGGELVKQ
                     LLRDTYDVAYPGVIAVYLDGCPKPGTGPQDIALAIIRAVFKNGYVKNKVMEFVGPGVA
                     SMTTDYRNGVDVMTTETTCLTSIWRTDEDTRNYLAKHHREADYKKLDPADVAYYDGCV
                     YVDLSTVKPMIALPFHPSNAFEIDELKANMKDILHMVEMDAEKVSGGKAHFTLLDKVV
                     GNDLMVQQGIIAGCAGGNYTNVIEAAHALRGKNCGCDEFSLAVYPSSQPVFSDLVKKG
                     AISDLMDAGAIIRTAFCGPCFGAGDTPCNNGLSIRHTTRNFPNREGSKPGNGQMAAVA
                     LMDARSIAATAANGGKLTSAWELDCWNDIPEYDFDDKSYKNRVYQGFGKAEEEKSLVY
                     GPNIKDWPEMSPLTDNILLKVCSKIMDPVTTTDELIPSGETSSYRSNPLGLAEFTLSR
                     RDPEYVGRTKEVDKLEKARVAEGEEKELALEPDLANVFAAIRTISGNENVSVKDTEVG
                     SMVYAVKPGDGSAREHAASCQSVIGGLANICKEYATKRYRSNVMNWGMLPFQMEA"
BASE COUNT          474 a          500 c          438 g          627 t
ORIGIN      
        1 ctgcttccat ctggaatggc agcatgcccc agttcataac attggagcgg tatctcttgg
       61 ttgcatattc cttacagata tttgcaagtc cgccgatgac actctggcag ctggctgcgt
      121 gctctcttgc agaaccgtca ccaggcttta ctgcatatac catacttcct acttctgtat
      181 cttttacgga aacattttca tttccagaaa tagtcctgat ggctgcaaat acatttgcaa
      241 gatctggttc taatgccagt tctttttctt cgccctcagc tactcttgct ttttctaatt
      301 tatctacttc tttggtccgt cctacatact ccggatctct tctggataag gtgaactctg
      361 ccagccccag cggattggaa cgataggaag aagtttcccc ggatgggatc agttcgtcgg
      421 tagttgttac cgggtccatg atcttggagc aaacctttaa aagaatgttg tcggttaatg
      481 ggctcatctc cggccagtct ttgatgttag gaccatatac aagggatttc tcctcttctg
      541 ccttaccaaa gccctgatat acacggttct tgtaagactt atcatcaaag tcatattccg
      601 gaatatcatt ccagcagtct aactcccatg cagaggtaag ttttcctccg tttgctgctg
      661 ttgctgcaat agaacgggca tccatcagtg ctaccgctgc catctgtccg tttcctggct
      721 tgcttccttc acggttcggg aagtttctgg tggtgtggcg gatacttaaa ccattattgc
      781 aaggtgtatc gcctgcgcca aagcaaggtc cgcagaaagc ggtacggatg atagcgcctg
      841 catccatcaa gtctgaaata gcgcctttct ttaccaggtc tgagaatacc ggctgggaag
      901 aaggataaac agccagtgaa aattcatcac agccacagtt ctttccacga agggcatgtg
      961 ctgcttcgat cacattggtg taatttccgc ctgcacagcc tgcgataatt ccctgctgta
     1021 ccatcaggtc atttccaact actttatcaa gaagtgtgaa atgcgctttt cctccggata
     1081 ccttttcagc atccatttct accatatgaa ggatatcctt catatttgcc tttaattcgt
     1141 cgatctcaaa tgcattgctt ggatggaagg gaagggcgat cattggcttt acggtactta
     1201 agtctacata aacacagcca tcgtagtaag ccacatctgc aggatccagt ttcttgtaat
     1261 ctgcctctct gtgatgcttt gccaggtaat ttctggtatc ttcatccgtt ctccagatgg
     1321 aggttaagca ggtggtctct gtagtcatta cgtctacgcc atttctgtaa tcggtagtca
     1381 tagaagcaac accaggtcca acaaattcca ttactttatt ctttacatat ccatttttaa
     1441 ataccgcacg gatgatggcc agcgcaatat cctgtgggcc ggtaccaggt tttggacatc
     1501 cgtctaagta aactgcgatg actcccggat aagctacatc atatgtatcg cgaagcagct
     1561 gttttaccag ttcgccgccg ccttcgccga ttgccatggt tcctaaagcg ccgtaacggg
     1621 tatggctgtc agatcctaag atcatctttc cgcagcctgc catcatttct ctcatgtact
     1681 gatgaatgac tgcgatatga ggtggaacgt agatgccgcc gtatttcttt gcagcggaaa
     1741 ggccaaacat gtggtcatct tcgttaatgg ttccgcctac tgcacaaagt gtattatggc
     1801 aattagtcag gacgtagggg atcgggaact tttccatacc ggacgctttg gctgtctgga
     1861 caattcctac gaatgtaata tcatgggatg ccatgctgtc aaaacggatc tttaaatgtt
     1921 ccatgttatc agctgtattg tgggcgctta aaatagaata agcaatggtt cccttttttg
     1981 cagcttcttt atctgctttc tttcctgtaa ggacttctac tttaacgctt tcctgttcc
//
LOCUS       DBMDUA010000321         1956 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_19682_length_1956_cov_2.359569, whole genome shotgun sequence.
ACCESSION   DBMDUA010000321 DBMDUA010000000
VERSION     DBMDUA010000321.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19682_length_1956_cov_2.359569"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="ACZPCL_13895"
     CDS             complement(<1..986)
                     /locus_tag="ACZPCL_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1163593.1"
                     /translation="MDMFVKLVNSANTFLWNVVLILLLCGTGIYYTFRLKFVQIREFK
                     QGLKQVFGGIGKKHEKKEGEMTSFQSVATAIAAQVGTGNLTGAATALIGGGPGAIFWM
                     WMSAFFGMATIYAEATLAQNYKTVKHGEVTGGPVYYIHAAFKGLFGKILGVIFAIFLI
                     LALGFMGNMVQSNSISTAFSQVFTSRNISIPPFTVGLLLAVVSLFVFIGGTNRLAAVV
                     EKLVPFMALVYIAGSLIVILCNITRLPSAVAQIFLGAFNPSAVLGGAAGVTVKEAIRY
                     GVARGLFSNEAGMGSTPHAHARAVVKNPHCQGLAAMISVFIDTFVILNLTVFS"
     regulatory      complement(1031..1146)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1249..1536)
                     /locus_tag="ACZPCL_13900"
     CDS             complement(1249..1536)
                     /locus_tag="ACZPCL_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163594.1"
                     /translation="MDNNWKQDPRLMAMDPEKVSLLMEFAEKTAHTDQSQLMPHFLHI
                     CQEANAKGLQFTNSETALLVDILSCHMPEKDRKKLPFLKLAAGKIAGGIHK"
     gene            complement(1550..>1956)
                     /locus_tag="ACZPCL_13905"
     CDS             complement(1550..>1956)
                     /locus_tag="ACZPCL_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163595.1"
                     /translation="TDYKLTDLDYLTGDHHLQMMKAALPFLNVPQQRTLSMFVKIQEL
                     KNTFQLFEDGQTAAMGICSLDQPEKPGSVKELLKTLKPYGNPGEQNLIDAANALLDGQ
                     TPLEQLRHFLTPAQQTRFETLQMVIAALQAMN"
BASE COUNT          571 a          412 c          439 g          534 t
ORIGIN      
        1 gaaaatacag tcagattaag gattacaaac gtatcaataa atacgctgat catagcagca
       61 agtccctggc agtgaggatt tttcacaacg gctcttgcat gtgcatgagg tgtggaaccc
      121 ataccggctt cattggagaa aagacctctt gcaacgccat agcggatggc ttcttttaca
      181 gtaacgcctg ccgcgccgcc taaaacagcg gatggattaa aagcacctaa aaagatctgg
      241 gcaacagcag aaggcaagcg ggtaatattg caaaggataa cgatcaggct tcctgcaata
      301 tatacaagtg ccataaatgg aaccagcttt tccacaactg cagccagacg gttggtgccg
      361 ccgataaata cgaacaggga aacaacggcc agaagaaggc caactgtaaa aggcggaata
      421 gagatattcc gtgaggtaaa tacctggctg aaagcagtgc tgatagagtt agactgtacc
      481 atattgccca taaatccaag agccaggatc aaaaagatgg cgaaaataac accaaggatc
      541 ttgccaaaga gtcctttaaa tgcagcatgg atgtagtaaa caggaccgcc tgttacttcg
      601 ccgtgtttta ctgttttata attctgtgca agagttgctt cggcataaat ggtagccatg
      661 ccaaagaacg cagacatcca catccagaag atagcgccag gaccgccgcc gatcagggca
      721 gtagcagcac ccgtcaggtt tccggtacct acctgggctg cgatggcagt agcaacagac
      781 tggaaggaag tcatttctcc ttcttttttc tcatgcttct ttccgatacc gccgaatacc
      841 tgttttaagc cctgcttaaa ttcgcggatc tgaacaaatt tcagacggaa tgtgtaataa
      901 ataccggttc cgcaaagcag aaggatcagc actacattcc ataaaaaggt gttagcagaa
      961 ttgaccaatt tcacaaacat gtccataatt agatagtctc cctttgtatg ttctacgtct
     1021 gtggatcgtc tgattccgcc ctgtcctttt gcctgagaga ttggcaggaa ttttctgcat
     1081 ttattcctgc tgtacacctt cggcgctcgt ttcagatatt gaaagagact ctccagagta
     1141 gtatatcgtc acggcatttg ccgcatgggg agactatagc atatataaaa taaaaatgca
     1201 ataattaaaa aagtataaaa aaacatacaa cggaaagaag ctaataatct atttatggat
     1261 cccgcctgcg atcttccctg cagctagctt aagaaacgga agttttttac ggtctttttc
     1321 cggcatatgg cagcttaaga tatctacaag aagggcggtt tcagaatttg taaattgcag
     1381 accttttgca tttgcttcct ggcatatatg cagaaaatga ggcatgagct gggactggtc
     1441 ggtgtgagcc gttttttctg caaattccat taataagctt actttttcag gatccattgc
     1501 cataagccgc gggtcctgtt tccagttatt gtccataaaa actccttttt taattcatag
     1561 cctgaagggc agcaatgacc atttgcaagg tttcaaaacg ggtctgttgt gcgggagtta
     1621 aaaaatgtct gagttgttct aaaggtgtct gtccgtctaa taaagcattt gctgcatcaa
     1681 tgagattttg ttctcccggg tttccataag gttttaaagt ttttaataat tcttttacag
     1741 aaccgggttt ttcaggctgg tctaaggagc agatccccat agctgcagtc tgaccgtcct
     1801 caaaaagctg aaatgtattt ttaagttcct gtattttcac aaacatggaa agggtacgct
     1861 gttggggaac atttaagaag gggagtgcag ctttcatcat ctgcagatgg tgatctcctg
     1921 taagatagtc caggtctgtc agtttatagt ctgtat
//
LOCUS       DBMDUA010000322         1886 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_20653_length_1886_cov_2.802801, whole genome shotgun sequence.
ACCESSION   DBMDUA010000322 DBMDUA010000000
VERSION     DBMDUA010000322.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20653_length_1886_cov_2.802801"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            143..796
                     /locus_tag="ACZPCL_13910"
     CDS             143..796
                     /locus_tag="ACZPCL_13910"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR1163596.1"
                     /translation="MLFVNPGESLAASKGAEVQTRSSYMVTIAAPSVEVHLSANEHSR
                     SVGQVKRGQTYEVLSNQGNGWVKISTGSGEGYIKTSGKASLVEKNRETVDLSARQRRQ
                     TVEFALQFVGGTYVFGGVDPNNGVDCSGFTRYILQNTASISLPHSSKGQSNYGTEVSE
                     EEMQPGDLIFYGDDAGINHVAMYIGNGQIVHASTEATGIKTSPYNYRKPVKIVSVLS"
     gene            1024..1746
                     /locus_tag="ACZPCL_13915"
     CDS             1024..1746
                     /locus_tag="ACZPCL_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HIR1163597.1"
                     /translation="MHNFFGKLVRTGLVCGILMTAVPMTSMAAIGPGFKTGTYIATIT
                     AESVNINKTKDGEDVLTTAKAGSVFEVLEDLGNGWMKIRVNDTEGYLPVSGNAEVEEA
                     EAGEMEQVQKEAIESSNSYKRQQLVSYALQFVGGPYRYGGSDPHTGTDCSGFTRYVYQ
                     HGLGISLNRSSGSQASQGTAISASKMQPGDLLFYGSGKSINHVAMYIGDGKIVHASTE
                     ATGIKVSNWNYKNPVKIVSVLG"
BASE COUNT          580 a          350 c          450 g          506 t
ORIGIN      
        1 aaagaaaaag acctgcggca gcctttaccg catgtctccg taacaattct atttcgacag
       61 ttaggagact ttcagcgtga attttgcttt atttcggaga catgcggtaa aggctgccgc
      121 aggtcttttt cttttatcag gtattctttt tgtaaatcca ggagaaagtc tggctgcttc
      181 taaaggggca gaagtacaga cacgctcttc ttatatggtg acgatcgcag cgccttctgt
      241 agaagtacat ctttccgcaa atgaacacag ccgttctgta ggacaggtaa aaaggggaca
      301 gacctatgag gtcctctcca atcagggaaa tggctgggtg aagatcagca caggcagcgg
      361 agaaggttat attaagactt caggaaaagc ttcccttgta gaaaaaaacc gtgaaacagt
      421 ggatctttct gccagacagc gccgtcagac tgtggaattt gctcttcagt ttgtaggcgg
      481 aacctatgta tttggcggtg ttgaccccaa taatggagta gactgctccg gttttaccag
      541 atatattttg cagaatactg cttccatcag tcttcctcat tcttccaaag ggcagtctaa
      601 ctatggcact gaggtttcag aagaggaaat gcagccggga gatctgatct tctacggtga
      661 tgatgcaggt atcaatcacg tagcaatgta cattggaaat ggacagatcg ttcatgcttc
      721 aacagaagct accggtatta agacttctcc ttataactac cgtaagccgg tgaagattgt
      781 cagtgtttta agctaggtca ttcaatcaga tcattacaaa tccctggtgc cagcaaggta
      841 ccggggattt ttttgtgaag aaattgtgaa atctgtcaaa aatattaata aatattaaaa
      901 aaaaataaaa atattgacga agctctcatc tgtgatataa tacaaatgta attaacgtaa
      961 tatttatgta acatttaaaa gcaactattt aacataaatc gtaacaaata ggatgggatg
     1021 agaatgcata atttttttgg taaactggtt agaacaggac ttgtatgtgg aatattgatg
     1081 acagcagtgc cgatgacttc catggcagcc atcgggcctg gatttaaaac aggaacatat
     1141 attgcgacta ttacagcaga aagcgtaaac atcaacaaga caaaagatgg agaagatgtt
     1201 ttgaccacag ccaaagcagg cagtgttttt gaagtattag aagaccttgg aaatggctgg
     1261 atgaagatcc gcgtaaatga tacagaaggt tatcttcctg tttccggaaa tgcagaggta
     1321 gaggaagcag aagcaggcga gatggaacag gtccagaaag aagccattga gtcctccaac
     1381 agctacaaaa gacagcagct tgtaagttat gcccttcagt ttgtaggtgg tccgtaccgt
     1441 tacggcggca gtgacccaca tacaggaaca gactgttccg gatttacccg ctatgtgtat
     1501 cagcatggtt taggtatttc tttaaaccgt tcttcaggca gccaggcttc ccagggaact
     1561 gcaatcagcg cgtccaagat gcagccggga gatctgcttt tttacggaag cggaaagagt
     1621 attaatcatg tagcaatgta cataggagat ggaaagatcg tacatgcttc taccgaagca
     1681 acaggtatta aggtttctaa ctggaattac aaaaatccgg tgaagatcgt aagtgtgctg
     1741 ggataatgaa ttaaaatgat attgaaaaat agacaggcct ttctgccggc ggcagaaggg
     1801 cctttttcgc aggttgggaa atttgtctca taaacggaag tggaacttat atttactcat
     1861 atcagggagg tgcttttgtg cagatt
//
LOCUS       DBMDUA010000323         1881 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_20725_length_1881_cov_1.481461, whole genome shotgun sequence.
ACCESSION   DBMDUA010000323 DBMDUA010000000
VERSION     DBMDUA010000323.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20725_length_1881_cov_1.481461"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="ACZPCL_13920"
     CDS             <1..163
                     /locus_tag="ACZPCL_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR1163598.1"
                     /translation="NEKQVISMRYGLFKGKEHTQREVAGVLGISRSYVSRIEKKAIGK
                     LREEFEKIQ"
     gene            284..1300
                     /gene="galE"
                     /locus_tag="ACZPCL_13925"
     CDS             284..1300
                     /gene="galE"
                     /locus_tag="ACZPCL_13925"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR1163599.1"
                     /translation="MAILVTGGAGYIGSHTCVELLNAGYDVVVADNLYNSSKKAVDRV
                     EQITGKKLKFYEVDLLDQPKVKEIFDKEEIEAVIHFAGLKAVGESVHKPLEYYNNNIT
                     GTLILCDEMRKHGVKNIVFSSSATVYGDPAQIPITEKCPKGEITNPYGRTKGMLEQIL
                     TDLHTADPEWNVMLLRYFNPIGAHESGLIGEDPKGIPNNLVPYIAQVAIGKLDHLNVF
                     GNDYDTPDGTGVRDYIHVVDLAKGHVKAMKKLEAKEGVSIYNLGTGVGYSVLDVLHAY
                     EKACGKTLKYEIQPRRDGDIATCYSDCTKAKEELGWVAEKGIEEMCADSWRWQSMNPN
                     GYRD"
     gene            1471..1848
                     /locus_tag="ACZPCL_13930"
                     /pseudo
     CDS             1471..1848
                     /locus_tag="ACZPCL_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564496.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
BASE COUNT          635 a          325 c          445 g          476 t
ORIGIN      
        1 aaatgaaaaa caggtcatct ccatgcgcta tggactgttt aagggaaaag agcacaccca
       61 gcgggaagta gcaggagttt taggcatttc caggtcctat gtaagccgga tcgaaaaaaa
      121 ggcaattggg aaattgaggg aagaatttga aaaaatccaa taaaaacaca taaaaaagag
      181 aaaaaatatg cattgttatt attgaggttt caggctatac tattacaaaa ggacaaaatt
      241 tgttcctggg aagaaagaga taagaaacga ggaggataat acgatggcta ttttagtaac
      301 cggaggtgcc ggatatatag gcagtcatac ctgtgtggag ttgttaaatg caggatatga
      361 tgtagtagta gcagataatc tttataactc cagcaaaaag gcagtggaca gagtagaaca
      421 gatcacagga aagaaactga aattctatga agttgatctt ttagaccagc caaaagtaaa
      481 agaaattttt gataaagaag agatcgaggc agtgatccat tttgcaggct taaaggcagt
      541 tggggaatct gtacataaac ctcttgaata ttataacaac aatattaccg gaactttgat
      601 cctctgtgat gaaatgagaa aacacggagt taaaaatatt gtattcagtt cttctgcaac
      661 tgtttacggt gatccggcac agatcccgat cacagaaaag tgcccgaagg gagagatcac
      721 caatccttac ggacgcacca agggaatgtt agagcagatc cttactgacc tgcatacagc
      781 agatcctgaa tggaatgtaa tgctgctgcg ttactttaac ccaatcggcg ctcatgaaag
      841 cggactgatc ggtgaagatc ctaagggtat tccaaacaac ctggtacctt atatcgcaca
      901 ggttgccatt ggaaaactgg atcatttgaa tgtattcgga aatgattatg atactccgga
      961 cggaaccggt gtaagagatt atatccatgt agtagacctg gcaaagggtc atgtaaaagc
     1021 catgaagaag ctggaagcaa aagaaggtgt cagcatctac aacctgggaa ccggtgttgg
     1081 atacagcgtt ttagatgtac tccatgccta tgaaaaggct tgcggaaaga ccttaaaata
     1141 tgagatccag cctcgcagag acggagatat cgctacctgc tattctgact gtacaaaggc
     1201 aaaggaagaa cttggctggg tagcagaaaa gggaattgaa gaaatgtgtg cagattcctg
     1261 gagatggcag agtatgaatc caaacggcta cagagattag ttttaagcat tcatagatcg
     1321 tagcacttta tatggcagta cagagaaact tttctgtgct gctatatttt tacctaaaag
     1381 caggaacaaa tgttcggttt ttccattgca tttctctgac ccatgtgata aaataagaaa
     1441 acaaacaaat gttcgggcgg tgatgagaag ttgatcatac aggaaaatct tacaatacag
     1501 gaaaaactga aaatactgac agatgcggca aaatatgacg tggcatgtac atccagtggt
     1561 gtacagcgcc gtggaaagaa aggtcatctg ggaaattctt ctgaggcagg tatctgtcac
     1621 agtttcgcag cagacggacg ctgtatttcc ctgctgaaga tcctttttac caatcagtgt
     1681 atttatgact gtaaatactg tgtaaaccgc tgtaccaatg atacagtgcg tactgcattt
     1741 actccagatg aagtctgcca gctgacgatg gaattttaca gaagaaatta tattgaagga
     1801 ctgtttttaa gttccggtat cctttctact gcagatcata ccatggagtt ttagctgctg
     1861 aaagtctggt ggaaagagta a
//
LOCUS       DBMDUA010000324         1860 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_21018_length_1860_cov_2.413303, whole genome shotgun sequence.
ACCESSION   DBMDUA010000324 DBMDUA010000000
VERSION     DBMDUA010000324.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21018_length_1860_cov_2.413303"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            136..1668
                     /locus_tag="ACZPCL_13935"
     CDS             136..1668
                     /locus_tag="ACZPCL_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HIR1163600.1"
                     /translation="MKPKQKSFLQNLDWVTTILPFGCITLLCCLFMIFPEGSANILAS
                     IRFFLGDQLGSYYLLMGLGAVICSLYMAFSRFGSIKLGDVEKPQYTAFQWGSMMFTAG
                     LAADILFYSLCEWMLYADESHISEMGSIQDWTSVYPLFHWGPIPWSFYIILAVAFGFM
                     IHVRRRNKQKYSEACRPLLGKKVDGVVGKLIDLTAVFALLAGTATTFSLATPLLSMAL
                     SKVLGVAETPLLTIAILVIICIVYTLAVYFGMQGVTRLAASCIYLFFVLLAYFLFLGG
                     QTRYIIETGITAIGLLTQNFISLATWTDALRTSSFPQNWTIFYWAYWMVWCVATPFFI
                     GTISKGRTIRQTVLGGYFFGLAGTFTSFIILGNYGLGLQTHGILDLMGLYRETGDLYQ
                     AIIAVFETMPAARFGLILLAVTMIAFYATTFDALTMVASSYSYRSLDADEEPDKKVKL
                     FWAITLMLFPIALIFSENSMSSLQSVSIVAAFPIGFIILMIVWSFFKDADAYLHDTGS
                     KG"
BASE COUNT          416 a          412 c          388 g          644 t
ORIGIN      
        1 cttacaaaat tgttacctat atgaaacttc tgtgaaatgt agcttgctat ttgatcttat
       61 tggtgctatc atgttcaata tgatttaatt ttttattttt ttgaacatat tattttttaa
      121 caggaggtca ttttcatgaa accgaaacag aaatcatttt tgcagaacct tgactgggtc
      181 accactatcc tgcctttcgg atgcatcaca ttgttatgct gtctgtttat gatatttccg
      241 gaaggctctg ccaatatcct tgcttccatc cgtttcttcc ttggcgacca gcttggcagc
      301 tattatctgt taatgggact gggagctgtg atctgttccc tgtatatggc attttcccgc
      361 tttggttcta ttaaactggg ggatgtagaa aaaccacagt atactgcttt ccagtggggt
      421 tccatgatgt ttacagccgg actggctgca gatatccttt tttattctct ctgtgagtgg
      481 atgctttatg cagatgagtc tcatatttct gagatgggtt ccattcagga ctggacttct
      541 gtttacccat tgttccactg gggtcctatt ccatggagct tttacatcat cctggctgtt
      601 gcatttggct ttatgatcca tgtgcgccgc aggaataaac agaaatactc agaagcctgc
      661 cgtcctcttc ttggaaagaa agtagacgga gtggttggaa aactgatcga ccttaccgct
      721 gtttttgctc tcttagcagg aactgccact accttttcac tggctacccc tcttttatcc
      781 atggctttaa gcaaggtgtt aggagttgca gagacgcccc ttcttaccat tgctatttta
      841 gtgatcatct gtattgtata cacactggct gtatattttg gcatgcaggg agtcacacgg
      901 ctggctgctt cctgtatcta tctgtttttt gtgctgttag cttacttcct gtttttaggc
      961 ggtcagacac gctacatcat tgagaccggg atcactgcca tcggactttt aacccagaat
     1021 tttatttctc tggctacctg gactgatgcc cttcgcacct cttccttccc acagaactgg
     1081 acgattttct actgggctta ctggatggta tggtgtgtgg caaccccgtt ctttattgga
     1141 accatcagca agggaaggac catccgccag actgttttag gcggctattt ctttggtctt
     1201 gctggcacct ttacttcttt tatcatattg ggaaattatg gtctgggact tcagactcat
     1261 gggatcttag atttaatggg actgtaccgg gagaccgggg atctgtatca ggcgatcatc
     1321 gctgtttttg agacaatgcc tgcagccagg tttggactga tcttactggc tgttaccatg
     1381 atcgcttttt atgcaactac gttcgatgcc cttaccatgg tggcaagttc ctattcctac
     1441 cgctccctgg atgcagatga agagccggat aagaaggtaa aactgttctg ggccattact
     1501 ttaatgcttt tccctattgc actgatcttt tcagaaaatt ccatgagcag tcttcagtct
     1561 gtatccattg tcgctgcttt tcctattgga tttattatcc tgatgatcgt gtggagcttt
     1621 tttaaggatg cggatgcata tttgcatgat acagggtcaa aaggttaaaa atctggtatc
     1681 ataacaaaaa caggagacgt ccccttccag gtctcctgtt tatacgttac tgtgcatttt
     1741 tacacttcct tgtctgttac atgtaatact catcctgcca taggatcttt cttatacaat
     1801 tatctgcctc tgtttcatct ctccccttaa aacatatggt cagaaaacca tataaactta
//
LOCUS       DBMDUA010000325         1795 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_21998_length_1795_cov_2.450413, whole genome shotgun sequence.
ACCESSION   DBMDUA010000325 DBMDUA010000000
VERSION     DBMDUA010000325.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21998_length_1795_cov_2.450413"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            95..409
                     /locus_tag="ACZPCL_13940"
                     /pseudo
     CDS             95..409
                     /locus_tag="ACZPCL_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NanQ anomerase/TabA/YiaL family protein"
     gene            728..1102
                     /locus_tag="ACZPCL_13945"
     CDS             728..1102
                     /locus_tag="ACZPCL_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1163601.1"
                     /translation="MIVLDYRDRRPLYEQVTEKLEELMFSGVLPPDSRLPSVRSMATE
                     LSINPNTIQRAYAELERQGYIYSIQGKGSFVSDGRYLAEGIRKDWEKQFDAAIEEGLR
                     IGVSYQDMIKILEEREGGKADA"
     gene            1095..>1795
                     /locus_tag="ACZPCL_13950"
     CDS             1095..>1795
                     /locus_tag="ACZPCL_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1163602.1"
                     /translation="MLKAVNLTKKFDDLVAVDHINAEIKSGSVFGLIGTNGAGKSTFL
                     RLAAGILKADEGHVTIDGEEVFENIKAKKKFFYIPDDAHFFSNASPLDMMEYYSVVYE
                     RFDKARFHKLMGNFGLDEKRKIHTFSKGMKKQVSVILGICSNTDYLFCDETFDGLDPV
                     MRQAVKSIFAAEIEDRNMTPVIASHNLRELEDICDHVGLLHKGGILLSKDLDDMKLNI
                     HKIQCVLKEGMTADD"
BASE COUNT          582 a          317 c          445 g          451 t
ORIGIN      
        1 ttggagtgct gaagggactg gatatataca cccgtcatgg tgaagcggta gtagtgccca
       61 acgtgaaagg tatgggagtg gcagaagctg agaaaatgtt accgcttaca ccactattcc
      121 gtgggaagag gctaagtatg aggcacatga gcattatacc gatatccagt acgtgatcga
      181 gggaagtgag gttatgacct acgctcctgc acatgaaatg actgtaaaga ctccttacaa
      241 tccagataag gacgtagtat tcttcgagaa taccacagac ggtctgcagg tagttgtaaa
      301 agctggtcag tatctcatct tcaatccatg ggatgcacat aagccgaagt cagctaacgg
      361 cgctccggca cctatcaaga aagttatcgt taagattaaa gaagattaat ggatcagtaa
      421 gttgatcgga tgatcaacag ataactctgg gaaatgtaaa tgaaataaaa taagtttacg
      481 aaaaatatcg tctgaacagg accatgaaaa atggactgta caggcgatat tttttattat
      541 ggctgaaagt gaagaaggct ttgtaatata ttcttaagaa aaaataccct tgtataaatc
      601 ataagataat gctatcttaa gtgtatcaca actgtatcaa tatgattagt acagttaata
      661 caattcatac agtcaaaaca gttcatatat atgttacagg tgaacagaca gaaaggagag
      721 aggctaaatg atcgtactgg attacaggga ccggcgtccg ctgtatgaac aggtgacgga
      781 aaaactggaa gagttgatgt tttcaggcgt attgccgccg gacagccgtc tgccgtcggt
      841 gcgcagtatg gcaacggagc tttccattaa tcccaatact atacagagag cctacgcaga
      901 attagaacga cagggatata tttattccat tcagggaaaa ggaagttttg tttcagatgg
      961 aagatatctt gcagagggga tacgcaagga ctgggaaaag cagtttgacg cagccataga
     1021 agagggactt cgcattggtg taagttacca ggatatgata aagatcttag aagagaggga
     1081 aggaggtaag gcagatgctt aaggcagtga acctgacaaa aaaatttgac gatctggtag
     1141 cagtggatca cattaacgca gagatcaaaa gcggttctgt atttggtctt atcggaacca
     1201 acggagcagg aaaatctacg tttctgcgtc tggcagccgg gatccttaaa gcagatgagg
     1261 gccatgtgac catagacgga gaagaggtat ttgaaaacat aaaagcaaag aaaaagtttt
     1321 tctatatacc ggacgatgct cattttttca gcaatgcttc tcctcttgat atgatggaat
     1381 attacagcgt tgtgtatgaa cgttttgaca aggcccgttt ccataaatta atgggtaatt
     1441 tcggcttaga cgaaaagaga aagatccaca ctttttccaa gggcatgaaa aagcaggtat
     1501 ctgtgatcct gggcatctgt tccaatacag attatctttt ctgtgatgag acctttgacg
     1561 gcctggatcc ggtaatgcgc caggcagtaa agagcatttt cgcagcagag atcgaagacc
     1621 ggaacatgac tcctgtgatc gcatcccaca acctgcggga actggaagat atctgtgatc
     1681 atgtagggct gctgcacaaa ggcggcatcc ttctttctaa ggatttagat gatatgaagt
     1741 taaatatcca taagatccag tgtgtattaa aagaaggaat gacggcagat gactt
//
LOCUS       DBMDUA010000326         1731 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_23085_length_1731_cov_2.542331, whole genome shotgun sequence.
ACCESSION   DBMDUA010000326 DBMDUA010000000
VERSION     DBMDUA010000326.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23085_length_1731_cov_2.542331"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="ACZPCL_13955"
     CDS             complement(<1..1398)
                     /locus_tag="ACZPCL_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR1163603.1"
                     /translation="MKVVGQGLNRVDAYGKVTGEAKYSADLEPRDILHGKVIHSTIAN
                     GLVKSFDLTEALKVPGVVKILTCFDAPDCQFPTAGHPWSVEAKHQDICDRRVLNQRVR
                     LYGDDIAAVVAENEVAAAQAARLVKVEYEEYEPIVTVKAAMAPGATPLHQDIRKDNVI
                     VHSHMTMGPKDFTFEDGLKQAKEKYKEEDLVEINEVYDTPRISHCHIELPVSWAYVDV
                     NGKVTVVSSTQIPHIVRRCTAQALGIPIGKVRIIKPYIGGGFGNKQDVLYEPLNAFLT
                     MSVGGRPVRLEISREETISGTRTRHAIEGVCRGLVTKDGTVLARQLDAYANNGGYASH
                     GHAICANCGNVFKDLYRDRLGAEIDCWTVYTSSPTAGAMRGYGIPQAAWFAECLTDDM
                     ATAIGMDPYEFRMKNCMEDGFVDPANGITFHTYGLKKCMEAGKKYIHWDEKRKEYANQ
                     TGPVRRGVGMSIFCYK"
     regulatory      complement(1607..1702)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT          441 a          439 c          330 g          521 t
ORIGIN      
        1 cttgtaacag aaaatagaca ttcccacacc acgtcttaca ggaccggtct ggtttgcata
       61 ctcttttctc ttttcatccc agtggatgta tttctttcct gcttccatac atttctttaa
      121 gccatatgta tggaatgtaa taccatttgc aggatctacg aaaccgtctt ccatacagtt
      181 cttcatacgg aattcatagg gatccatgcc aatagctgtt gccatatcat ctgtaagaca
      241 ttctgcaaac catgctgcct gtgggatacc atatccacgc atagcaccgg cagtcggtga
      301 agaagtataa acagtccagc agtcaatctc agcacctaaa cggtctctgt acagatcctt
      361 aaatacgttt ccgcagttag cgcagattgc atggccatgg gaagcataac ctccgttgtt
      421 tgcataagcg tctaactgtc ttgcaagaac cgtaccgtcc tttgttacca gaccacggca
      481 tacaccttcg atggcatgac gggttctggt tccggaaatg gtctcctcac ggctgatctc
      541 cagtcttaca ggtcttccgc ctacgctcat tgtcaggaac gcatttaacg gttcatacag
      601 cacatcctgc ttatttccaa atccgccgcc gatatatggc ttgataatac ggactttacc
      661 gatcggaata cccaacgcct gcgctgtaca gcggcgtaca atatgcggga tctgggtaga
      721 agaaacaacg gttacttttc cgtttacatc tacataagcc caggaaacag gaagttcgat
      781 atggcagtgg gaaatacgtg gtgtatcgta tacttcattg atctccacca gatcttcctc
      841 tttatatttt tcttttgcct gttttaagcc gtcttcaaag gtaaaatcct ttggtcccat
      901 agtcatatgg gagtgaacga tcacattatc tttacggata tcctgatgca gtggtgttgc
      961 ccccggtgcc atagctgcct ttacggtaac gatcggctca tattcctcat attctacctt
     1021 taccagacgg gctgcctgtg cagctgctac ctcattttca gctacaactg ctgcaatatc
     1081 atctccatac agccttactc tctggtttaa tactcttctg tcgcagatat cctgatgctt
     1141 tgcctctacg ctccatggat gacctgcagt tggaaactgg cagtccggtg cgtcaaagca
     1201 ggtaaggatc tttacaacac caggtacctt taatgcttct gtaagatcaa agctttttac
     1261 aagaccattt gcaatagtag aatggatcac ctttccatga aggatatccc ttggctctaa
     1321 atctgcgctg tatttcgctt cgccagttac ttttccatat gcatcaacac ggttcaatcc
     1381 ctgacctaca actttcataa acgcttcctc ctttactaac cttatgttct cccggaatct
     1441 ttctgtactt aatcatgtga gaagtttttt gtctgctgta cctgagagga cagcccctat
     1501 ctgatgaaaa acagcccttt ccacttcccc attttgacct gccaaacccc atcgcagatg
     1561 cttacaggat aactacggaa aatgtaccct gagggtagaa aaaaagagct gactacataa
     1621 actatgtagt caactcttaa ggtagttggt agagactctg ggccgatccc caggatatac
     1681 atatcagaca attggtttat ttgattgtct ttattatata tctaactatt t
//
LOCUS       DBMDUA010000327         1703 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_23559_length_1703_cov_2.074282, whole genome shotgun sequence.
ACCESSION   DBMDUA010000327 DBMDUA010000000
VERSION     DBMDUA010000327.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23559_length_1703_cov_2.074282"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            14..919
                     /locus_tag="ACZPCL_13960"
     CDS             14..919
                     /locus_tag="ACZPCL_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1163604.1"
                     /translation="MERRKLTGKDKVFNVFNYGVFGLLTLICAYPFYYLIINTLSAND
                     LSAAGEILFFPKGFHLDNYKQVLKLNGIFNAAVISVARTVLGTALSVLASAFMGFMFT
                     QERMWGRKFWYRFVVITMYFNAGMIPMFITMKNLHLTNTFWVYVIPTIVQPFNIILVK
                     TYVESLPKSLQEAAELDGAGVLMIFWKVILPVCTPILATIAIFTAVGQWNSFQDTLIY
                     ITDQKLYSLQYLLYQYINQASSLAMTVKNSGGGAMNMASLATQQTSTSIRMTVSVVVV
                     LPILFIYPMFQKHFVKGIMMGAVKG"
     gene            980..1117
                     /locus_tag="ACZPCL_13965"
     CDS             980..1117
                     /locus_tag="ACZPCL_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163605.1"
                     /translation="MKKTGKKWMALLMAFSMAALTACGSSEKTARSVLPRDAFLREST
                     I"
     gene            1201..1494
                     /locus_tag="ACZPCL_13970"
     CDS             1201..1494
                     /locus_tag="ACZPCL_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163606.1"
                     /translation="MDKDGRAYTNTLVDGKVYGPDGTRIQSDNGWDIVTVEEDVYKDS
                     DYSGADLKENAKPVIMAGSSVIVNNSGKLKKNGKVKLDGVTYQVKNYAVQVTE"
BASE COUNT          520 a          315 c          403 g          465 t
ORIGIN      
        1 aaggagaaag ggaatggaaa gaagaaaact gacaggaaag gataaggtgt ttaatgtttt
       61 taattacggg gtatttggtc tgctgacact gatctgtgcc tatccgttct attatctgat
      121 catcaacact ttaagcgcca atgacttaag cgctgcggga gagatcctgt ttttcccaaa
      181 gggctttcat ttggataatt ataagcaggt attaaaatta aacggtattt ttaacgcggc
      241 tgtgatctcg gttgccagaa ctgtccttgg aacagccctt tctgtgcttg catccgcgtt
      301 tatgggattt atgtttaccc aggagcggat gtggggaaga aagttctggt atcgctttgt
      361 agtgatcacc atgtatttta atgcaggtat gatccccatg tttatcacca tgaaaaacct
      421 gcatctgacc aatacattct gggtctatgt gatccctacc attgtacagc catttaatat
      481 tatcctggta aagacctatg tggagtcatt gccaaaatct ttacaggagg cagcagagct
      541 ggacggggcc ggtgtcctta tgatattctg gaaagtgatc cttcctgtat gtacaccgat
      601 cctggcaacc attgctattt tcacggcagt gggacagtgg aattctttcc aggatacatt
      661 gatctacata acagaccaga aattatattc ccttcagtac cttctttacc agtatatcaa
      721 ccaggccagt tctctggcga tgacagtaaa gaacagtggc ggcggtgcca tgaacatggc
      781 gtccctggca acacagcaga cctctacttc cattcgtatg acagtttctg ttgtggtagt
      841 tctgcctatc ttgtttattt atcctatgtt ccagaaacat tttgtaaagg gaattatgat
      901 gggagctgta aaaggatgat gtttttgatc ccggaaagtt ttacaagggg acatcattaa
      961 ttaaaggagg aacaaaaact tgaaaaagac aggaaaaaaa tggatggctc tgctgatggc
     1021 attttccatg gcagctctta cagcctgcgg aagcagtgaa aagacagcga gatcggttct
     1081 tccacgagat gccttcctgc gggaatctac tatttaagcc agaatgaaaa ttctttagga
     1141 cagcttcaga cagacgggga attaaagctg acaggcggaa ctgaccggct ggcctattac
     1201 atggataaag acggacgggc ttatacaaat acactggtcg atgggaaagt atatggcccg
     1261 gacggaaccc ggattcagtc agataatggc tgggatattg taaccgtaga agaggatgtt
     1321 tacaaagaca gtgactacag cggtgccgat cttaaggaaa atgcaaaacc tgttataatg
     1381 gcaggaagca gcgtgatcgt aaataacagc ggaaagctga aaaaaaatgg aaaagtaaag
     1441 ctggacggtg ttacatacca ggtaaaaaat tatgcagtcc aggtaacaga ataaacagag
     1501 gtttaagaag gatcatctca gagtaccaca ggtaccggaa gagatggtcc tttttgtgta
     1561 catttatgga taattattgg aatttattga aaaacattca tcagtaagct tcctgtataa
     1621 ttcaaagtgt agagagtaaa tgattttaca aaaatagata cctaagttaa actgtcattg
     1681 ctgaccggcc agttagcaaa tga
//
LOCUS       DBMDUA010000328         1445 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_29497_length_1445_cov_2.236607, whole genome shotgun sequence.
ACCESSION   DBMDUA010000328 DBMDUA010000000
VERSION     DBMDUA010000328.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29497_length_1445_cov_2.236607"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="ACZPCL_13975"
     CDS             complement(<1..311)
                     /locus_tag="ACZPCL_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HIR1163607.1"
                     /translation="MVTLHSGKQIIIDNKRIASKEEAAAVSQEEAFKNTLTYQVLKKH
                     NHSDNMEHLQIQFDSMGVYDNTYVGILQTAYASGLKEFPMPCVFTNCHNCLCAVGGTI
                     NT"
     gene            complement(384..1376)
                     /locus_tag="ACZPCL_13980"
                     /pseudo
     CDS             complement(384..1376)
                     /locus_tag="ACZPCL_13980"
                     /inference="COORDINATES: protein motif:HMM:NF021778.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
BASE COUNT          355 a          335 c          283 g          472 t
ORIGIN      
        1 gtattaatag tgccgcctac tgcacaaagg cagttatggc agttggtaaa tacacagggc
       61 attggaaatt cctttaaacc gctggcataa gcagtctgaa ggatacctac ataagtatta
      121 tcatatactc ccatggagtc aaactggatc tgtaaatgtt ccatattatc agaatggtta
      181 tgcttcttta acacctggta agtcagtgta tttttaaatg cctcttcctg tgagacagct
      241 gctgcttctt cttttgatgc aatcctctta ttatcaataa tgatctgttt tccgctgtgt
      301 aaggtgacca tactcattct ccttttcttc tataatttat atgtattaac atgtcctgat
      361 ctgaaatttt cccacatctt gtactaacag gttttccatg caatgcacac cggactttct
      421 gtagcgatct tccttcctgt atctttcaac gttccatctt ttaggatctc aaaaacagta
      481 atggtatcag aaagctcatt tgctgccaaa agtctggtgc cttccgggtt taatgtaata
      541 aatcttggct gctctccgcc tgttttgata ttctggatat aagtcatatt tcctgttgct
      601 tcatccagat gataaaccgt aatgctgtca tgttttctgt tggaaacata cagtgtatga
      661 ccttccggat ggatatcaat ggcacttgcc cagccatctc ctgcataatc ttccggcaga
      721 ctggtaagaa gctgttttaa tgccagctta cctgtctttt catcaaaatc ataagctgct
      781 acggtactgt ctttttcatt tacacagtaa cagtatttat ttcccggatg gaacacgcag
      841 tgtcttggtt ccgcaccggt acggcctttc tggataaatg tttctttcag acttccctgt
      901 tcttcgtcaa tggcaaacac ggtcagtttc ccgactccct gcagtcgccc ctgggaaggg
      961 accaccagcc agcgtccgct tctgtctttc atggtctggt gaggatggga aatatagccg
     1021 ggaccgccat ttcctgaaat aaattccagg tcatgaagtt ttcccaaaga tccatcttca
     1081 agaactggga tcacagatac gcttcccgtc tggagattgg ctacataaag ccatctttct
     1141 tctgggtcta atgtaagatg caccggattg gtcccatatg tttttactgt attaaggtac
     1201 tctaaggtac cattttcccg gatcttaaat gcgcttacct ggcaaaaatc tccatgtatg
     1261 ctgtacagac ggtcctgtgc tttattcaga cagagataag aaggatttac ctgaccttcc
     1321 aggatctgta ccagctccca gttatctcca tcaaccttgt atacactgat cccttttcct
     1381 ctggcttttc gctcttttgt ggtacggcag aggaaaaggg atcagtgtat acaaggttga
     1441 tggag
//
LOCUS       DBMDUA010000329         1368 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_31884_length_1368_cov_2.007893, whole genome shotgun sequence.
ACCESSION   DBMDUA010000329 DBMDUA010000000
VERSION     DBMDUA010000329.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31884_length_1368_cov_2.007893"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="ACZPCL_13985"
     CDS             <1..713
                     /locus_tag="ACZPCL_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR1163608.1"
                     /translation="FATISAYGANAAMCHYSATKESDTVLEDHGFYLVDSGGQYYEGT
                     TDITRTIALGELTEEEKEHFTLVLISMLRLKEVKFLYGCRGLSLDYVAREPFWSRGLN
                     FDHGTGHGVGYLLNVHERPNGIRFRMVPERHDNGVLEAGMITSDEPGLYIEGSHGIRT
                     ENLILCVKDEKNQYGQFMKFEFLTFVPVDKDAVVKELMTERDIALLNAYHKEVFEKIS
                     PYMDDQEKEWLRKATAPI"
     gene            complement(801..1295)
                     /locus_tag="ACZPCL_13990"
     CDS             complement(801..1295)
                     /locus_tag="ACZPCL_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HIR1163609.1"
                     /translation="MRTYDLLDSLGITYTRADHDTLPTMEACQEVGKLLGIEICKNLF
                     LRNTQKTAFYLLILPGSKKFKTAALSKQIGSARLSFAEPEFMEQYLNITPGSVSVLGL
                     MNDPENKVRLLIDKDILENHPYFGCHPCINTSSLKFRTEDLLNKILPAIHHNYTIVDL
                     PYAE"
BASE COUNT          366 a          233 c          386 g          383 t
ORIGIN      
        1 gctttgctac aatctctgct tatggggcga atgcggctat gtgtcactat tctgcaacaa
       61 aggaaagtga tacggtactg gaagaccatg gcttttatct ggtagactct ggtggccagt
      121 attatgaggg aactacggat attacccgta ccattgcgtt aggagaactg acggaggaag
      181 aaaaagagca ttttactctg gtccttataa gcatgctgcg attaaaagaa gtgaaatttt
      241 tatatgggtg cagaggctta agccttgatt atgtggcaag agagccattt tggagcaggg
      301 gactgaattt tgaccatgga accggtcatg gtgtgggcta tctgttaaat gttcacgaaa
      361 gaccaaatgg catccgcttc cgtatggtgc cggagcggca tgataatggt gttctggaag
      421 ctggaatgat cacttctgat gaaccgggac tttatattga aggaagccat ggtatccgta
      481 cagagaacct gatcctttgt gtaaaggatg agaagaacca gtatggccag tttatgaagt
      541 ttgagttcct tacctttgta ccagttgata aggatgcggt agtgaaagag ctgatgacag
      601 agagagatat tgctcttctt aacgcatatc acaaagaggt gtttgagaag atcagtccgt
      661 atatggatga ccaggagaaa gagtggttaa gaaaggcaac ggccccgatc taagggatcg
      721 tttaagggac cgttgcgtct gcttcaggta tttgtacaga ttgggaatgc gttaaagatg
      781 tatctgctaa gtttgatgga ctattctgcg tatggaagat ctacaatggt atagttatgg
      841 tggatagcag ggaggatctt gtttaacagg tcttctgtgc ggaatttcag gctggaggtg
      901 ttgatacatg gatgacatcc gaagtaagga tggttctcca ggatatcttt gtctatgagc
      961 agccggactt tgttttctgg gtcgttcatc agaccgagaa cacttacaga accgggagtg
     1021 atgttaaggt actgttccat aaattccggt tcggcaaagg aaagacgggc gctgccgatc
     1081 tgcttggaga gagcggctgt tttgaacttt ttgctgcctg gaaggatcag cagatagaag
     1141 gcggttttct gggtattgcg aagaaatagg tttttgcaga tctcaatgcc taaaagctta
     1201 cctacttcct ggcatgcttc catagtgggt agtgtgtcat ggtctgcacg ggtataggtg
     1261 atcccaaggg agtctaacag gtcataggtg cgtatctcct ttggaagacg gtcggtagta
     1321 tctggaaaaa gggtttaggt acttccatac ctataataat aagacagc
//
LOCUS       DBMDUA010000330         1353 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_32343_length_1353_cov_3.544728, whole genome shotgun sequence.
ACCESSION   DBMDUA010000330 DBMDUA010000000
VERSION     DBMDUA010000330.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32343_length_1353_cov_3.544728"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="ACZPCL_13995"
     CDS             <1..563
                     /locus_tag="ACZPCL_13995"
                     /inference="COORDINATES: protein motif:HMM:NF040019.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YczE/YyaS/YitT family protein"
                     /protein_id="HIR1163610.1"
                     /translation="TRAGLGTSPVSGLPFVLSLVTPVSMGFFTFLFNMIFLICEALLR
                     KRFSVEQALQIPITFFFSFCIDKAMAIIPTRFGGPWGDSFVYLVIGCIIMSLGISFEV
                     IADVIMLPAEAFVRALAKKTGFIFGNVKVGFDSILTILAMILALICFHKLNGVREGTL
                     INALVVGQLVKQWRRCIEKPLSNAIA"
     gene            complement(711..>1353)
                     /locus_tag="ACZPCL_14000"
     CDS             complement(711..>1353)
                     /locus_tag="ACZPCL_14000"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HIR1163611.1"
                     /translation="KDSSWLLSWTINRQGQFKAQKKDEICVWVYSLFTDVPGDYIKKP
                     MKECNGKEITAEWLYHLGIPTEEIDDLAANHANCTPTMMPYITAFFMPRSAGDRPDVI
                     PDGCVNAAFLGQFAETPRDTIFTTEYSVRTAMEAVYGLCGVDRGVPEVWGSVYDVRDL
                     LDSTVKLMDGKSPLEMKLPLPLEVLKKPLLRKIDKTVVGKLLRDHNVIKDWML"
BASE COUNT          322 a          339 c          289 g          403 t
ORIGIN      
        1 tcacccgtgc gggtctgggg acttcaccgg tttcagggct tcctttcgtg ctcagtctgg
       61 taacacctgt atcaatggga ttttttacct ttctgtttaa tatgatcttc ctgatctgtg
      121 aagcactctt aagaaagcgt ttcagcgtag aacaggcttt gcagatcccg attactttct
      181 ttttcagttt ctgcattgac aaggccatgg ccatcattcc tacccgtttc ggcggtccct
      241 ggggtgactc ttttgtctac ctggtcatcg gatgtatcat catgtccctt ggcatcagct
      301 ttgaggttat tgcagatgtg atcatgcttc cggcagaggc atttgttcgc gcccttgcta
      361 aaaagaccgg ctttatattc ggaaatgtaa aagtaggttt tgacagcatc cttaccattc
      421 tggcaatgat cctggcactg atctgtttcc ataagttaaa cggagtcaga gaaggaacac
      481 tgatcaatgc cctggtagtt ggccagttag taaaacagtg gagacggtgc attgaaaaac
      541 cgctgtcaaa tgcaattgca taaaatatca aaaatcccgg tccctgtaaa tggggatatg
      601 tcatgaaaca aacggaaccc gccctgccag gcgggttttt gcatattggc cacctttttg
      661 caacagaaaa acgcgcccgc aggcgcgtct ttcccgatta cttttcttct ttataacatc
      721 cagtctttga tcacattgtg gtctcttaac agttttccaa cgactgtctt atcgatcttt
      781 ctaagcagag gcttcttaag cacttccagt ggcagaggca gcttcatttc cagcggagat
      841 tttccgtcca tcagctttac agtactgtct aacagatctc tcacatcgta tacgcttccc
      901 cagacctcag gaacaccacg gtctacaccg caaagtccgt atacagcttc cattgcagta
      961 cgtacagaat actccgtagt gaaaatcgta tctcttggtg tttcagcaaa ctggcctaag
     1021 aatgcagcgt ttacgcagcc atccgggata acgtccggtc tgtcccctgc gcttcttggc
     1081 ataaagaaag ctgtgatata tggcatcatg gtcggagtac agtttgcatg gtttgctgcc
     1141 agatcatcga tctcttctgt aggaataccc agatgataca gccactctgc tgtgatctcc
     1201 ttaccattac actctttcat tggcttcttg atataatcgc ctggaacatc ggtaaacagg
     1261 ctgtataccc atacacagat ctcatctttc ttctgtgctt taaactgacc ctggcggttg
     1321 atagtccagc ttaacagcca gctagagtcc ttg
//
LOCUS       DBMDUA010000331         1306 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_33974_length_1306_cov_2.174274, whole genome shotgun sequence.
ACCESSION   DBMDUA010000331 DBMDUA010000000
VERSION     DBMDUA010000331.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33974_length_1306_cov_2.174274"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="ACZPCL_14005"
     CDS             <1..1149
                     /locus_tag="ACZPCL_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HIR1163612.1"
                     /translation="TFYGTMGGQQGDKGTIHSANGEFKVEETIHLQGGKIGHVGVMTK
                     GMLSIGETVTLSVCPKNRALTSKNHSATHLLQKALRTVLGSHVEQAGSYVDAGRLRFD
                     FTHFSAMTPEEIKKVEDIVNEEIQAALPVVTEVMTLDEAKKTGAMALFGEKYGEKVRV
                     VKMGDFSTELCGGTHVANTSTIGYFKIFSEAGIAAGVRRIEALTSDGLMKHYAEVEAE
                     LHEAAKAAKTTPAALTAKIQSLLDEIKALNSENEKLKSKMANDSLGDVLSQVKEVNGV
                     KVLASKVADVDMNGLRNLGDQLKDKIGEGVVVIASVMDGKVSLMATVTDEAQKKGAHA
                     GNLIKAIAGLVGGGGGGRPNMAQAGGKNPAGIEEALKKAVEVVEEQTK"
BASE COUNT          411 a          242 c          348 g          305 t
ORIGIN      
        1 acattctacg gaaccatggg cggccagcag ggcgataagg gtaccatcca cagtgcaaac
       61 ggtgaattca aggtagaaga gaccattcat ttacagggcg gaaagatcgg ccatgtaggt
      121 gttatgacta agggaatgtt aagcattggc gagacagtta ccttatctgt atgcccgaag
      181 aaccgtgcat taacaagcaa gaaccacagt gctacccact tactgcagaa ggcactgcgt
      241 actgtattag gaagccatgt agaacaggct ggttcctatg tagatgcagg acgtctccgt
      301 tttgatttta cccatttctc tgctatgact ccagaggaga tcaaaaaggt agaggatatt
      361 gtaaatgaag agatccaggc tgcgcttcca gttgtaacag aagttatgac tttagatgaa
      421 gcaaagaaga ccggtgctat ggcactgttt ggtgagaagt acggcgaaaa ggtacgcgtt
      481 gtaaagatgg gagatttctc cacagagctt tgcggtggta cccatgtagc aaatacaagc
      541 accatcggat acttcaagat cttttctgag gcaggtatcg cagccggcgt aagacgtatt
      601 gaggctctta cctctgacgg tctgatgaag cattatgcag aagttgaagc agagcttcac
      661 gaagcagcta aggcagctaa gacaactcct gcagctctta cagctaagat ccagtccctg
      721 ttagacgaga tcaaggcatt aaactctgaa aatgagaagt taaagagcaa aatggcaaat
      781 gactccttag gcgatgtatt aagccaggta aaagaagtaa atggcgttaa ggtacttgct
      841 tccaaagtag ctgatgtaga tatgaacggt cttagaaatc tgggtgatca gttaaaggat
      901 aagatcggtg aaggtgtggt tgtgatcgct tccgttatgg acggaaaagt aagtctgatg
      961 gcaactgtaa cagatgaggc tcagaagaag ggcgctcatg caggcaacct gatcaaggct
     1021 attgctggtc ttgtaggtgg tggcggcggc ggccgtccaa acatggctca ggcaggcgga
     1081 aagaacccgg ctggtattga agaagcctta aagaaagccg tagaagtagt agaagaacag
     1141 accaaataag cctgtttgaa taaaaagtgc aaaaaacgca gaaaaatata aaatttttct
     1201 tgacgtatgg gaattttatg ctataatcat agcagtgcta aaaataccca aacagttgta
     1261 ttatgcacaa ttacacaact ggagggaggt taggtgtgag cgatgt
//
LOCUS       DBMDUA010000332         1159 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_39932_length_1159_cov_2.789225, whole genome shotgun sequence.
ACCESSION   DBMDUA010000332 DBMDUA010000000
VERSION     DBMDUA010000332.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39932_length_1159_cov_2.789225"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="ACZPCL_14010"
     CDS             <1..852
                     /locus_tag="ACZPCL_14010"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1163613.1"
                     /translation="ARVAEKLYISPPALSKSLKRVEEELGEVLFVRAPSGLSLTFAGE
                     CFLKYAYQIQKMYEEMSMEFCELNQMKKGVLRIGVTERMGSLLLPQVLERFRTKYPNI
                     QLDIVEKGGVDLEEMLAKGGLDMALVSLPLKYPSLPSHVFYEDPFLIAVPKDHPMNEV
                     WKEKEALPVTVLKGQELLLTRRFKKTRLIAEQILEPLKGDYRIITESYSIETIIRLAA
                     ENMGISLVPEIYAKAYDCGDRLQYYRIENCPVTWKWAVVFSSDPQNLTRPSKELYKIL
                     SLIHFPV"
     gene            complement(826..>1159)
                     /locus_tag="ACZPCL_14015"
     CDS             complement(826..>1159)
                     /locus_tag="ACZPCL_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR1163614.1"
                     /translation="DHAAVRTAHYEEAQKFFEDVFDMHMWREIGEKPGRKCWFKEGIQ
                     LCETSEIAVSSENGYDHISLAVDSVPEIMEKIKAYPVTPINDHWFSLPNGTKIELKLL
                     SDWKVNEG"
BASE COUNT          332 a          230 c          271 g          326 t
ORIGIN      
        1 gcaagagtgg cggagaaatt gtatatttct ccgcctgctt tgagtaaaag tttaaagagg
       61 gtagaagagg aactggggga agtcctgttt gtaagggcac cttccggtct ttctttgact
      121 tttgcaggag aatgtttttt gaaatatgcg tatcagatcc aaaaaatgta tgaagaaatg
      181 tccatggaat tctgtgaact aaaccagatg aaaaagggcg ttttgcgcat tggggtaaca
      241 gaacggatgg ggtccctgct tttgcctcag gttcttgagc gttttcggac aaaatatccc
      301 aatatccagt tagatatcgt ggaaaaaggg ggagtggact tagaagagat gcttgcaaaa
      361 ggtggtctgg atatggcact ggtatcgctg cctttaaaat atccaagcct tccaagccat
      421 gtcttttatg aagatccttt tttgattgcg gtacctaaag atcatcctat gaatgaagtg
      481 tggaaagaaa aagaagcact tcctgttact gttttaaaag gtcaggaact gcttcttacg
      541 cgcagattta aaaagacccg tctgatagca gagcagatct tagagccttt aaagggggat
      601 tacaggatta tcacagagtc ctacagtatt gagaccatca tccgtctggc agcagaaaat
      661 atggggatat ccctggtgcc ggagatctat gcaaaagcct atgactgcgg agaccgcctg
      721 cagtattacc gcattgaaaa ctgtccggtc acatggaaat gggccgttgt attcagttca
      781 gacccgcaaa atctgacccg gccgtcaaag gaactttata aaatattatc cctcattcac
      841 tttccagtct gacaaaagct ttaattcgat ctttgttcca tttggaaggg agaaccagtg
      901 gtcgttaata ggggtaacag gatatgcttt gatcttttcc atgatctctg gaacagaatc
      961 tacagcaaga gagatatggt cgtagccgtt ttcagaagaa acggctattt ctgatgtttc
     1021 gcataactgg atcccttctt tgaaccagca tttgcgtccc ggtttttcac cgatctcccg
     1081 ccacatatgc atatcaaaaa catcctcaaa aaacttttgt gcttcttcat aatgagctgt
     1141 acgtacagca gcatgatcg
//
LOCUS       DBMDUA010000333         1010 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1268
            NODE_48147_length_1010_cov_1.579758, whole genome shotgun sequence.
ACCESSION   DBMDUA010000333 DBMDUA010000000
VERSION     DBMDUA010000333.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508367
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.7292x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:55:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,805
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,705
            CDSs (with protein)               :: 2,705
            Genes (RNA)                       :: 55
            rRNAs                             :: 3 (23S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 38 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48147_length_1010_cov_1.579758"
                     /isolate="MAG1268"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="ACZPCL_14020"
     CDS             <1..434
                     /locus_tag="ACZPCL_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1163615.1"
                     /translation="HLKDLLDVTLINPGQRMTLGSRENMETEKEDYPFKVTREETDRY
                     LREVKDQNPIHQGEGAIVPGFLMANKILKQLSPQIPFQAEFRFLTPLQIERSGWLEFL
                     SSDEENDPKIEAEKALKKVQKIHLRTPQNIILKAEITEYKE"
     gene            451..>1010
                     /locus_tag="ACZPCL_14025"
     CDS             451..>1010
                     /locus_tag="ACZPCL_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR1163616.1"
                     /translation="MSQTASKTVHTTKSYSIWNIRSLTIMALMTALLIVSTYIKIPLP
                     FSTASITAQTLMVNLIGLLLSPLQTLAVMVTWILLSVVGIGGSLGKLLGPAGGYRYGN
                     LVAAVLISMFCCKVKKVRWQTAFIIIVGIPVIYLFGAVQMKYVTNQPWGAIMIQAVLP
                     FIPLDIVKCFAAAGIAKPLRRLFPTD"
BASE COUNT          306 a          195 c          228 g          281 t
ORIGIN      
        1 cacatctaaa agatctttta gatgtgactt tgatcaaccc gggtcagagg atgactttgg
       61 ggagcaggga aaatatggaa acagaaaaag aagattatcc ctttaaggtc accagggagg
      121 agaccgatcg ctatttaagg gaagtaaagg accagaatcc cattcatcag ggggaaggtg
      181 ctattgttcc gggttttctg atggcgaata agatcttaaa acagttatca ccccaaatcc
      241 cttttcaggc agagttccgt tttcttaccc ctctgcagat tgaaagatcc gggtggcttg
      301 aatttttatc ttcagatgaa gaaaatgatc cgaagataga agcggaaaag gcattaaaaa
      361 aggtacagaa aatccatctg aggacgcctc aaaatattat tttaaaagct gaaattacag
      421 agtacaaaga gtagaaagga gcggacagct atgagtcaga ccgcatcaaa aacagttcac
      481 acaacgaaat cttacagtat ttggaatata cgaagcctta cgattatggc tttaatgaca
      541 gcacttctta ttgtttcaac gtatattaaa ataccgcttc ccttttcaac agccagtatt
      601 acagcccaga ctttaatggt aaacctgatc ggtctgctcc tttctccgtt acagactctg
      661 gcagttatgg ttacatggat cctgttaagt gttgtgggaa ttggcggatc tttgggaaaa
      721 ctgttaggac cggcaggcgg ataccgttat ggcaacctgg tggcagctgt tctgatctcc
      781 atgttctgct gtaaagtaaa gaaagtaaga tggcagactg catttatcat tatcgtagga
      841 attccggtaa tctacttatt tggtgcagtt cagatgaagt atgtgaccaa tcagccatgg
      901 ggcgctatta tgatccaggc agtgcttccg tttattcctc ttgatattgt taaatgtttt
      961 gcagcagcag gaattgcaaa acctcttcgc agactgtttc caacagacta
//
